LOCUS       DAOSDR010000001        25205 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrimonas sp. isolate AMR_MDS_1826
            NODE_887_length_25205_cov_4.513519, whole genome shotgun sequence.
ACCESSION   DAOSDR010000001 DAOSDR010000000
VERSION     DAOSDR010000001.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863355
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrimonas sp. (wastewater metagenome)
  ORGANISM  Petrimonas sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Dysgonomonadaceae; Petrimonas.
REFERENCE   1  (bases 1 to 25205)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 25205)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:10:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,720
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 35
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25205
                     /organism="Petrimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_887_length_25205_cov_4.513519"
                     /isolate="AMR_MDS_1826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2023866"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1414
                     /locus_tag="PLI30_00005"
     CDS             <1..1414
                     /locus_tag="PLI30_00005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019538157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI77967.1"
                     /translation="SLQGFSDEQIDYFYHYLETENPDWLRGVVSGPGSPSLSDTRYRL
                     PGKYMHRHYPDITHTVRCLYPTLRWDQAFALTLGREPVNPQPFYYAELHDRYAPFTDG
                     FLTYSDGAHDDLNKFVWSRKGWSMEEDAAGITEQYVRFFFGAKPDDRVTDAILGLERN
                     WDGPIEVNGGIEMTFLRWRQLEEAYPRLQSDWRWQMLLLRAYYDTYTKRRKLFEQRLE
                     REAGKLLGKVSEMGAAGAMKQALEKVNEADRVNIAPSLRKKIDDYCDALYKSVGLQTS
                     VEKYQASGAERGCVLDFADYPLNNRWWLSDRFEKIGQMETEAEKLAALEVIARWENPG
                     KGSYYDNISDISRSPRVKTTVYDAADVAWWDGGMSRKRLSTQLFQNFPKLLYEDLDPK
                     ARYLIRTAGYGDALLRVDGERISPTLYNKELEEFKEFPIDRRFFQDGKLEVSFDEPEE
                     SHLNWRQYSKICDIWLIKIL"
     gene            1460..2557
                     /locus_tag="PLI30_00010"
     CDS             1460..2557
                     /locus_tag="PLI30_00010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019538154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase"
                     /protein_id="HOI77968.1"
                     /translation="MKKIQLLLLIVLSVFFAQCASTRNKIEEKAAPRVKNVIVMIGDG
                     MGVSQVYAGLTANKGTLFLEKSQFIGFSKTYSASNYVTDSAAGGTAIACGTKTKNGAI
                     GVDPDGSPVKSMLTYAAENGLSTGIVVSCAVTHATPASFVAHQPRRDMEEEIAVDFVN
                     SDLSVFIGGGKKYFDSRSDKENLLDKLSVKGFQIAFNLDDIKKVGSGKLAGLVADEHP
                     APYPERGEFLPESVQTAINLLKNNEKGFCLMVEGSQIDWACHTNDKEATINEMLDFDR
                     AIKVAFDYADQDPNTLVVITADHETGGLSLTGGDLSTGEVEANYGTKRHTAVMVPVFA
                     YGAGAAEFAGIYENTDIFTKVLKLYRMRSPR"
     gene            2669..3838
                     /locus_tag="PLI30_00015"
     CDS             2669..3838
                     /locus_tag="PLI30_00015"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="HOI77969.1"
                     /translation="MKSFSIVTLLLVTALSCSSYPSEKHNTEPTSGIKHGIVYYQENK
                     YGGWPANNGIWIWDNEILVGFVEADYKPADGLHTYNQSSAHHKYARSTDGGETWTIED
                     AYEQGQTAWGNDHSIAVDKAETPVALKTPVEDFTDPGFIITFVRHNNNNGPTHFYYSM
                     NKGGVWSGPYSFPDLGTAGIANRTDYIVESDQELSVFLTTAKTNKKEGRVAFAKTTDG
                     GINWEFVSWITDEQGGFDIMPSSLRLSDTELITIIRTRTAERQDLLTAYRSSDNGKSW
                     ARLKNPVNDTGNGGSPPALVKLQDGRLALAYIYRSVYGSRVNVRFSSDNGESWSDEVV
                     LRSGDGANRDAGYPRMVQRPDGKLVLIYYWNNANDEDSTPYRYIASTIFDPGKWK"
     gene            3886..5337
                     /locus_tag="PLI30_00020"
     CDS             3886..5337
                     /locus_tag="PLI30_00020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010535638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter"
                     /protein_id="HOI77970.1"
                     /translation="MQLTVIDIVIFLVFTLGTVLFGSSFLRKNKTAGDFMTAGKGMSG
                     FVVGMSIFATYVSSISFLALPGNAYTGNWNSFVFSLSIPVAIFFAARYFVPFYRSINS
                     PSAYSFLEERFGKWARLYASSCYLLTQIARIGSVLFLLALPLNAMLGWSIPLIIILTG
                     VCVVLYAALGGIKAIVYTDAIQGLILIAGALACLVILMVSMPGGGDELLRIGMEYDKF
                     SLGSLGLTLNEPTFWVTLIYGLFINLQNYGIDQNYVQRYKTARNEKSARFSALFGGLL
                     YVPVSLLFFFIGTALFVYYKTQPGLLPETVTGDQVFPYFIVNGLPAGFTGLLIAAIFA
                     AGMSTVSTSINSSATVVLTDFFNVTSHSEADEKRKMNILHATSVILGALGVGVGLAMM
                     SVKSALDAWWSMAAIFSGGMLGLFFLGYIARGIKGSYAFVGVICGVLLIAWMSLSKQT
                     VFHNYLTIVLGTLVIFTVGMLLAIAGSRLKKGN"
     gene            5486..7006
                     /locus_tag="PLI30_00025"
     CDS             5486..7006
                     /locus_tag="PLI30_00025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009011167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylsulfatase"
                     /protein_id="HOI77971.1"
                     /translation="MDKKIRILAFGATAFSALYAQQKPNIVLIYADDIGYGDLSCYGA
                     TRVQTPYVDALANNGVRFRNAHSAAATSTPSRYGLFTGEYPWRRKGTGIAAGDAALII
                     KPDRYTLPKMMKEAGYATGAVGKWHLGMGAETGKQNWNERVSPGPAEIGFDYSYIMAA
                     TGDRVPCVYMENQRAVGLDPKDPIEVSYTKNFPGEPTGKDNPELLTKLKPSHGHDMAV
                     VNGISRIGFMKGGKSALWEDENIADSITVHAIRFIERNKDNPFFLYFGTNDIHVPRYP
                     HGRFRGKTDMGYRGDAILQFDWSVGEVVRALKEAGVYDNTLIIITSDNGPVVDDGYQD
                     EAVEKLKNHKPWGPFRGGKYSTFEAGTRVPFIVHWTGNVCPGVSNALVSQIDMLGTLA
                     ELAGIDLAEAGQFDTQKHLPAWLGNDPKGRDYIIGSAGSLTLLTRDWKYIEPNKGNAY
                     SAHTNTELGNNPEDQLYNITIDRGEYDNVAVENPLMVKFMKQILEEEKAKGTGLEL"
     gene            complement(7477..8703)
                     /gene="nhaA"
                     /locus_tag="PLI30_00030"
     CDS             complement(7477..8703)
                     /gene="nhaA"
                     /locus_tag="PLI30_00030"
                     /inference="COORDINATES: protein motif:HMM:NF018648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaA"
                     /protein_id="HOI77972.1"
                     /translation="MTQHLHPTGTKSRKFTITDFLHDSRAVGVLLLLCTTASLILTNL
                     PGIGESYNHFWETEIPFLHSIHLPHSILHIINDALMALFFFQVGMEIKRETVIGELST
                     PSRMMLPMVAALFGVIFPAIIFLTATKGTIYQSGWAIPAATDIAFSLGILSLLGKSVP
                     HSMKIFLTALAIIDDLCAILIIAFFYGSQPEFMWLLGVAACIILIVLVIKYLRNPLSR
                     ILYLSLGVVMWYCMYQSGIHATFAGVILSALLPVKKIPVYEKVLLFPVNFLIIPIFAL
                     ANTSIFINGSAIANLLGELSIGIILGLLIGKPAGISLAVYLMTKTRIIKLKTKPNWML
                     YIGVGILAGIGFTMSIFVSMLAFDDKSLQDTAKLAVLVASTLSMIIGYTWLRIALKKK
                     KEEKLENLSPEEMIAG"
     gene            complement(8715..9422)
                     /gene="pepE"
                     /locus_tag="PLI30_00035"
     CDS             complement(8715..9422)
                     /gene="pepE"
                     /locus_tag="PLI30_00035"
                     /EC_number="3.4.13.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010265897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepE"
                     /protein_id="HOI77973.1"
                     /translation="MRLLLISNSTNPGEAYLDYPKEHIKNFLGDRAKNAIFIPYAAVT
                     FSYDEYEEKVNNRFAEIGHHVTGIHRFINPVEAIENADAIVVGGGNTWQLVKILQEKG
                     LMKVIRKRVKKGTPYIGWSAGSNIACPTLKTTNDMPIVEPDKFKTLKLVPFQINPHYL
                     DDHPANHGGETREVRINEFIEVNRDLFVVGLREGTMLLLEDKELVLVGERNARIFKFG
                     QEPRELSHEDDFNFLLS"
     gene            9529..10197
                     /locus_tag="PLI30_00040"
     CDS             9529..10197
                     /locus_tag="PLI30_00040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006800112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HOI77974.1"
                     /translation="MNEILQQIRTDLRRSMNGIASKSMREKGLHYKLNFGVDVPRLRE
                     LSKRYPIDAQLAELLWRQETRELKILATMLYPVHEFDMDKADEWVKEIPNHEIREQVS
                     MNLFQKLDFADKLVQKWTDSKDEEVRTSGYWLFARLLIVKSGLVNKTNQNEIMEKMIS
                     DLSAGSYFLRLSAQRALTFLGRTTESFSKKILDGIQDLQTSGDAAKKEIFDSLSFEFT
                     DFPD"
     gene            complement(10178..10816)
                     /locus_tag="PLI30_00045"
     CDS             complement(10178..10816)
                     /locus_tag="PLI30_00045"
                     /inference="COORDINATES: protein motif:HMM:NF024854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HOI77975.1"
                     /translation="MKSKKLLLEIIELLDQFEKTTQSTEVELSLNDFILFLQQRRTIK
                     ENRNDTVRIAQNISFLHRYSKFYIKKALKGSLLQTVDEYTYLVALFNEESLSKTEINN
                     RNVIEKTSGNEVMRRLLKAKLIGERRDEEDKRRMRVFITDKGRAELTKVFPGLWKSAT
                     MLSDVLAPPEKELFLQASDKLCDFHKNIFIHCKEEEIDSLISKLPLVNRENP"
     gene            11027..11725
                     /locus_tag="PLI30_00050"
     CDS             11027..11725
                     /locus_tag="PLI30_00050"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOI77976.1"
                     /translation="MEVAARIEEAGKEYKTGDTTIVALAPSTTVFNKGEVTLIVGPSG
                     SGKTTLLSLLGCVIYPTTGNVWLGDTCVNHLSEAELAKIRLNEIGFVFQGFNLLAPLN
                     ALENVMQPLLLKGESRKVAKQKSLSIIRKFDMQSRVKNLPRNLSGGQQQRIAVARALV
                     SDPKLILCDEPTASLDHKSAVLVMDMLKALSREDRAVIIVTHDTRLKKYADRTIYVSE
                     GKISDTPFEEEFDH"
     gene            11743..12666
                     /locus_tag="PLI30_00055"
     CDS             11743..12666
                     /locus_tag="PLI30_00055"
                     /inference="COORDINATES: protein motif:HMM:NF027890.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HOI77977.1"
                     /translation="MKKLFIGCFLILLLVGCGNKTENRVSVEVSPVTVAVGIGKIVPQ
                     GGVSKLASPVAGIVVEIAVATGDKVKSGDLLLTLDNTDASLALSEINSRLVTQQKSIQ
                     SAELMKEQGLTRLREKERKLNDARELFDAGAVTGENVRNLQNDYDLEKQGQEKLQNDI
                     LLQESQLREIVSQKNMRSEELSRTSLRAPMDGIVLDVLPKKGEAVNRYETYMMLAPDA
                     PLIVQAEIDEMFSNRLALGQSCEIRVAGNPQPVAQGKILSISPDLKKKSLFSDSGQDL
                     QDRRVREIEISIDKDTNLLIDTKVECMIHLN"
     gene            12673..13809
                     /locus_tag="PLI30_00060"
     CDS             12673..13809
                     /locus_tag="PLI30_00060"
                     /inference="COORDINATES: protein motif:HMM:NF014717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HOI77978.1"
                     /translation="MFATAYKFIRFEKSKSTGILLGIIISIYLIGLELGIFFYLTTLI
                     GGVINNAKPEYSQVFVVNRLTNNANILSPFDKRWVNQLRSIEGIDDAHGMVMANVSVR
                     FDNGESSPAIIIGSEYPELAAGPSESLLNSGSTNNLFLHGTVSTDFYDNKTFGYDVKQ
                     GTSFEISGKNVTVGATTKNAKGFSSPMLYTTDATARYYSGFSEDAVNAVIVTVKDESK
                     IDEVIERINTVAPDLRAWRSEELKNATVINVMTANNMGMSFGTLVIFAIVSGFFIIGL
                     TMYSATYDRIKDYGTLKAIGATGRHITLLVITQSFIYAVVGFAVSLLLLVGTKFGMAK
                     AGLIINLSPQFLAFLFFVTLIIAVASSMFSIQKLKKVEPSSVFR"
     gene            13837..15186
                     /locus_tag="PLI30_00065"
     CDS             13837..15186
                     /locus_tag="PLI30_00065"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HOI77979.1"
                     /translation="MKKTYILSLAWIVSVLSVLGQEKKINLNALTLEEAVKYSLDHSP
                     TFIAQQLKEQQAGYKLSQVKLDYLPDIYVTSDLRRNIIIPSTPIPASMINPSAPEDEL
                     MYMRFNTPWSSGAGLNVTFDIFNPETAGLKSEQEKQLKISQLDSRIAENELRATVSQA
                     YIDCAIAQTQLDAITADTVYYAALSREIEDLYRREKVSLVDKNNAEMNYNASLARFHQ
                     AKNIQHNSRINLLMYLGVEPDEDKLDQLRLSDDIQSLYSKMMSGKVLSGENPLSQRRQ
                     AELVSLSEIKTKNALWKYAPSLSLSGYYGANYFGKELKLGNTNRWFGNSFVALSLQIP
                     ISRSLSTAKEVSRLRIQEQLERENLRDVKNNRLGELSRELARLETCKNTYRLKQSNMK
                     LITQNIVAKQAQLQKGYTLESEFLSEKLREQNALQEYLQAAYDLLSAYINLEKLTKN"
     gene            complement(15193..15384)
                     /locus_tag="PLI30_00070"
     CDS             complement(15193..15384)
                     /locus_tag="PLI30_00070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI77980.1"
                     /translation="MATKKQIFTAMWAIIVVIAIASIVCLIVLPKWKGIFLASGGGFL
                     IVNIFISMFFIQNNYRDKK"
     gene            complement(15389..16780)
                     /locus_tag="PLI30_00075"
     CDS             complement(15389..16780)
                     /locus_tag="PLI30_00075"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HOI77981.1"
                     /translation="MKKKAIILFLFLVSVSVSAQEITGSWSGTLNVMGNKLPVVFHVS
                     KTDSSYSTTMDSPAQNALGLPTSKTSFVENKLEIVATGLGIFYQGTLEGDSILGTFNQ
                     GGIPFPLVLKLTDKPVLNRPQEPQPPFPYKTEEVKFIHKKDSIELAGTLSVPDSAGTF
                     PAVILIAGSGPHDRDETIFGHKPFLVIADHLTRKGFVVLRYDKRGTGRSTGYYPIATT
                     QDFATDVAAAVEYLKAREEVDKSAIGLIGHSEGGVIAPMVASKNRDIKFIVLMAGMGE
                     RGIETIMEQNRMALELLNIEPENSDQSLKVIRQMLESLSEWKGTEADRVALRDQLSQL
                     WEQYPILVKMKLKKDAFIRDQFNTIATPWYRQFLALDPAEYLKKVKCPVLAINGEKDT
                     QVLAEKNLGAIKSALDKGRNYRYETKTYPGLNHLFQECETGRADEYGKIEQTLSLDVL
                     SDITVWIRKQLNN"
     gene            complement(16794..17267)
                     /gene="rlmH"
                     /locus_tag="PLI30_00080"
     CDS             complement(16794..17267)
                     /gene="rlmH"
                     /locus_tag="PLI30_00080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005854430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HOI77982.1"
                     /translation="MKVVLLSLGKTDEDFYVQAMDIFRKRLSHYLPFDLEFVPDVKNT
                     KNLSEKEQKNLEGEAMLSRIQQGDYVVLLDDKGKQYSSVEFSRYIEKKSHSVPKRLVF
                     VVGGPYGFPDEVYRRANEKLSLSRMTFTHQMVRLVFVEQLYRAMTILNGEPYHHE"
     gene            17420..18268
                     /gene="nadC"
                     /locus_tag="PLI30_00085"
     CDS             17420..18268
                     /gene="nadC"
                     /locus_tag="PLI30_00085"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013619253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0019363 - pyridine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HOI77983.1"
                     /translation="MELKKLTDKLVDLAFAEDIGDGDHTTLCSIPETAFGRARLLIKE
                     EGILAGVEVARTIFHRFDEGLVVEVLINDGTYVKPGDVAFIVSGKVQSLLQTERLVLN
                     IMQRMSGIATTTGKYVKLLEGTKTKVLDTRKTTPGMRMLEKQAVKIGGGENHRIGLFD
                     MILLKDNHVDFAGGIENAIRGAQKYLKEKNKQLNIEIEVRNFDELNQALAVGGVDRIM
                     LDNFTPEQTCEAVKIVDGRVELESSGGITFETIRQYAECGVDYISVGALTHSVKSLDM
                     SLKAIE"
     gene            18282..18707
                     /locus_tag="PLI30_00090"
     CDS             18282..18707
                     /locus_tag="PLI30_00090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004301773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dCMP deaminase family protein"
                     /protein_id="HOI77984.1"
                     /translation="MMTDTKQLELDKRYMRMAFIWSENSYCKRRKVGAILVKDKMIIS
                     DGYNGTPSGFENVCEDEDNVTKPYVLHAEANAITKVARSNNSSEGATLYVTSSPCIEC
                     AKLIIQAGIRKVVYADSYRLSDGVELLRRADIELISVDL"
     gene            18733..20427
                     /locus_tag="PLI30_00095"
     CDS             18733..20427
                     /locus_tag="PLI30_00095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019541256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HOI77985.1"
                     /translation="MSPEKKIKTWLPLWVGLGIALGILIGSIYSQFGNTGKVDGTGKI
                     DAIFNYINKSYVDTVNIRQLVEEALPKIVQELDPHSAYISASEMKRLNEDLEGHFSGI
                     GVSFYVLSDTIVVTSIVPGGPSEAAGIQQWDRIVNVNDTLIAGRKITNADVLQKLRGE
                     KGSEVKLGIKRNDDTKLIDITVTRGDIPMNSVQAAYMQTNTIGYIKVGTFGFNTFNEF
                     ISALSKLKSQGAHSFVIDLRGNSGGSLEIVVAMVNEFLHKGDLIVYADGRNFPRQDSY
                     ANGSGTSKEDDVVILIDELSASASEIFAGAIQDNDRGLVMGRRSFGKGLVQSQRNFPD
                     GSAVRLTVARYYTPSGRSIQRKYEKGKYDEYELEALNRYMQGDYVNSDTASTLIPFKT
                     EGGRTVFGGDGIMPDVFIARDTIGMNSYFNTIANKDFIQEYAVTYSDMHKHKLSEFQS
                     AEDLARHLYKQPLLMNLVSYADNKGVRPRPYYIEESRKLFERHLVAYIVRNFFGEESY
                     FSVYMQDDVLMKNAVDFIERGLAKPESVVQEKYKSISLGLRVSFPVPKEFGSLFYV"
     gene            20433..20996
                     /locus_tag="PLI30_00100"
     CDS             20433..20996
                     /locus_tag="PLI30_00100"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006842863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030272 - 5-formyltetrahydrofolate
                     cyclo-ligase activity [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HOI77986.1"
                     /translation="MNITEQKKRLRERIAQKKQSYSQRELLNFSEEVISTLELTEVFQ
                     NAKVVLAYYSMSDEVNTHELIKKYCLQKHFLLPVVNNGELILKKYVSEESMSTSAYGI
                     KEPVGDTFPESEYGKIDLVIVPGVAFDRTLNRLGRGKGFYDRLLPKIKAPKTAICFDF
                     QVFDNIPVSQGDIKMNMIVCQNEIIVE"
     gene            21111..23066
                     /locus_tag="PLI30_00105"
     CDS             21111..23066
                     /locus_tag="PLI30_00105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009132219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate sugar
                     epimerase/dehydratase"
                     /protein_id="HOI77987.1"
                     /translation="MNKFLENYLSNRVLSRMSILIIDILIILFSTLTMYFLRFGFEGL
                     TTQVKADGITTTLVLVFFNVISFLIFKTFSGVLRFSAFSDLVRIIYALTLGYFISFIC
                     LMVIKKVDPTFTVSNTIHFATYVLNVLLMIFSRIVVKEVYESITGKPQKATNIFIYGT
                     KQAGISLAKAIRGNREFKYKVLGFITDEDNMVGKNLLGLTIYANNENLFRVLEAKDVK
                     TVIVSPRKMDQIRNSPLLENFVDHNISLLTTSPVNEWNGTLTGKEQLKDIQIEDLLPR
                     NPININLMEIAAHIEGKRVMVTGAAGSIGSEIVRQVASFNPFNIILIDQAETPLHDMR
                     LELQKKWIDINTNIIVADVGNATRMERIFAKTRPQYIFHAAAYKHVPMMEDNVSESIQ
                     TNVLGTKILADLAVKYGAQKFVMISTDKAVNPSNVMGCSKRISEIYVQSLSRHLEKNG
                     QKTTQFITTRFGNVLGSNGSVIPLFREQIKNGGPLTVTHPEIIRYFMTIPEACQLVLE
                     AGSMGKGGEIYLFDMGKPVKILDLAKRMIRLSGSQNIKIEFTGLRHGEKLYEELLNAS
                     ENTIKTHHEKIMIARVREYEYEKVKDQIEGLIEISYQYDDMRTVKKMKEIVPEFQSIN
                     SPYEAVDRLLEKLEDKESVKIQDAFSI"
     gene            complement(23058..24893)
                     /locus_tag="PLI30_00110"
     CDS             complement(23058..24893)
                     /locus_tag="PLI30_00110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019541258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOI77988.1"
                     /translation="MNGIITILKRFLPPYKKYVVLSFLFNLLTALLNVFSLATIIPIL
                     QVLFKVNDKVFEFIPWETKGVSLIDIVLNNGNWYMARLIETHGGSTTLLFLAIALIVM
                     TLFKTGTAYFGSYFTIPIRTGVVKDIRNKINDKILVLPIGFFSEERKGDILARISGDV
                     NEVENSVMSSLDMLFKNPILILIYLITMIVLSWQLTLFVFVVLPIMGYVMGTVGKSLK
                     RSSFEAQNKWGELMSQIEETLSGLRIIKAFNAESKISKRFYTGSDTFRKMSNRIARRQ
                     YLAHPMSEFLGTLTIAIVLWFGGSLILSGNGIIDAATFIYYLTIFYSIINPIKEFSRS
                     AYAVQRGLASMERIDKILEAQNDITEIQHPQKLSFKNNIHYRNVWFKYNKEWVLKGID
                     LKIEKGKTVALVGQSGSGKSTMTDLLSRFYDIQEGGVFIDNINIKDVTLHDLRSKIGY
                     VTQEAILFNDTFFNNIAFGLKEVTSEEVIEAAKVANAHEFIMATQHGYQTNIGDRGGK
                     LSGGQRQRISIARAVLKNPEILILDEATSALDTDSERLVQDALEKLMRNRTTIVIAHR
                     LSTIKNADEIYVLKEGQIIESGGHNSLYELNGYYTKLCNMQGDLN"
BASE COUNT         7151 a         5209 c         5917 g         6928 t
ORIGIN      
        1 ttcattgcag ggtttcagcg atgagcagat cgattatttt taccattatc tggagacaga
       61 aaatccggat tggttgcggg gagtggtaag cggtccgggt agcccttccc tgtctgacac
      121 gcgctaccgg ttacccggga agtatatgca ccgccattac cccgatatta cccatactgt
      181 ccgctgcctc taccccaccc tccgctggga tcaggcgttc gcccttacct tgggccggga
      241 gccggtcaac ccgcaaccgt tttattacgc ggagttacac gaccggtatg cccctttcac
      301 cgatggtttc ctcacctact ccgacggagc gcatgacgat ctgaacaagt tcgtatggag
      361 ccgcaaagga tggagtatgg aggaagatgc cgccggtatc acggagcaat atgtacggtt
      421 cttctttggc gccaaacccg atgatcgggt aacggacgcc atactgggat tggaacgcaa
      481 ctgggacggc cccatagagg taaacggggg gatagagatg actttcctgc gctggcggca
      541 gcttgaagag gcctatcccc gcctacaaag tgactggcga tggcagatgc tgttgctccg
      601 cgcttattat gacacttata ccaaaaggcg gaagttattc gaacaaaggt tggagcggga
      661 agccggtaaa ttgctcggga aggtctccga aatgggtgct gccggggcga tgaaacaggc
      721 tttggagaaa gtgaacgagg ccgaccgggt aaatattgcc ccttcgttga gaaagaagat
      781 cgatgattat tgcgacgcgt tgtataaatc ggtaggtttg cagacaagcg tggagaaata
      841 tcaggccagc ggcgctgaac gaggctgtgt gctcgatttc gcggactatc cgctgaacaa
      901 ccgctggtgg ttgtccgacc ggtttgaaaa gatcggacaa atggaaaccg aagccgaaaa
      961 gctggccgct ttagaggtga tcgcccgatg ggaaaatccc ggaaagggaa gttattacga
     1021 taacatatcg gatatcagcc ggagtccgcg ggtaaaaaca acagtctatg atgctgccga
     1081 tgtggcctgg tgggacggcg gaatgagtcg taagaggctg tctacccagt tattccagaa
     1141 cttcccgaaa ctcctgtacg aggatcttga tcccaaagcg cgttacctga tccgtaccgc
     1201 cggatacggc gatgcgttgc tcagggtgga tggagagcgt atatccccta ccctctataa
     1261 taaggagctg gaggaattca aagagttccc catagataga agattctttc aggacggtaa
     1321 gctggaggtt tcattcgatg aaccggaaga gtctcacctg aactggcgtc aatattccaa
     1381 gatttgtgat atctggctta taaaaattct ttgacaacac gctactggaa tcattcgtta
     1441 agttttaaat atttgtaata tgaaaaaaat tcaattattg cttcttatcg ttttatcggt
     1501 attctttgca caatgtgcat ctacacgtaa taagatcgaa gaaaaggcag ccccgcgcgt
     1561 aaagaacgtt attgtgatga tcggagatgg catgggtgtc agtcaggtgt atgcgggatt
     1621 gacggcaaat aagggaacac tcttcctcga aaagagtcaa tttataggct tctcgaagac
     1681 ctactccgca agcaattatg tgacagattc ggctgcggga ggtactgcca tcgcatgtgg
     1741 aaccaaaacg aagaatggcg ccattggtgt cgaccccgat gggagtccgg ttaaatcaat
     1801 gctgacgtat gcggcggaaa acgggttgtc cactgggatt gtggtgagtt gtgctgtcac
     1861 ccatgctacg cccgcttcat ttgtcgcaca tcagcctagg cgtgatatgg aagaggagat
     1921 tgcggtcgat tttgtgaatt ctgatctttc ggtttttatt ggaggaggaa agaaatactt
     1981 cgatagtcgc tctgacaagg agaatctgct cgataaactc tccgtcaaag gttttcagat
     2041 cgctttcaac ttggacgata tcaagaaagt cggctcagga aaactggcgg gtttggtagc
     2101 ggacgaacat ccggctccct atccggaaag gggggaattc cttcccgaaa gtgtacagac
     2161 ggctatcaat cttctgaaaa acaatgaaaa aggtttttgt ctgatggtgg aaggttccca
     2221 aatcgattgg gcttgccata cgaatgataa ggaggctaca atcaatgaaa tgttggattt
     2281 cgatcgggct atcaaagtgg ctttcgatta tgccgaccag gaccccaata cgcttgtcgt
     2341 tattaccgcg gaccatgaga caggaggcct gtcgcttaca ggaggtgacc tgtcgaccgg
     2401 agaagtggaa gccaattatg gtacaaaaag gcatactgca gtgatggttc ccgtctttgc
     2461 ctacggtgcg ggcgctgccg aatttgccgg catatacgag aataccgata tttttaccaa
     2521 agttttaaag ctctatcgaa tgcgttcacc ccggtagtct cacttttttg gactcatcgg
     2581 cggcaacatt ctaaaatctg tgatatctgg ctgttaaaac ggaattaaaa aagaatatac
     2641 atacacgaac attaaacgat aaaaaggtat gaaatcattt tccattgtaa ctttattatt
     2701 agtaacggcg ctatcctgta gcagctatcc aagtgagaaa cacaatacgg agccaacgtc
     2761 aggcatcaaa cacggcattg tctactacca ggaaaacaag tatggcggtt ggcctgcgaa
     2821 caacggtata tggatctggg acaatgagat attagtggga tttgtcgagg cagactataa
     2881 accggccgac ggcttacata cgtataacca gtcatcagca caccataaat acgcccgcag
     2941 taccgacgga ggagaaacct ggacgataga ggacgcttac gaacaggggc aaacggcttg
     3001 ggggaatgat cacagcatcg ctgtagacaa ggcggaaacg ccggtagctt taaaaacacc
     3061 ggtggaagac tttaccgatc ccggctttat cattacattt gtccgccata acaataacaa
     3121 cgggcccacc catttttatt attccatgaa caaagggggt gtgtggtcgg gcccctattc
     3181 ctttccggat ttaggtacgg caggaatagc aaaccgtacc gattacatag tggaaagcga
     3241 tcaggaactc agcgtgtttc ttactacggc aaaaacaaac aaaaaagaag ggcgtgttgc
     3301 ttttgcgaaa acaaccgatg ggggtataaa ctgggagttt gtctcgtgga ttaccgatga
     3361 gcagggagga ttcgatatta tgccttcatc gctgcggtta tcggatacgg aattgataac
     3421 tatcattcgt acgagaacag ccgaaaggca ggatttactg accgcttacc gttcttccga
     3481 taacggtaag tcgtgggcca gattaaagaa tcctgtaaac gatacgggga acggaggctc
     3541 tccgccggca ctggtcaaac tgcaagacgg acggttggcc ttggcctata tttatcggag
     3601 cgtatacggt tcccgtgtaa atgtgcgttt cagctcggat aacggagaaa gctggagcga
     3661 tgaggttgtg ctcagaagcg gcgacggcgc caaccgcgat gccggttatc cgcgcatggt
     3721 acaaagacca gacgggaaat tggtattgat ctactactgg aacaatgcga acgatgaaga
     3781 ttcaaccccg taccggtaca tcgcatccac cattttcgat cctggaaaat ggaagtaaag
     3841 gcaacaggtt ttgcaataga aaacacttta aattaattat actttatgca gttaaccgtt
     3901 attgatatcg ttatttttct tgtttttacc ctaggaactg tactgttcgg gagttcattt
     3961 ttgcggaaaa ataaaaccgc tggtgatttt atgaccgcag gaaaggggat gtctggattt
     4021 gtggttggga tgtctatctt tgccacgtac gtaagtagca tcagttttct ggcactgccc
     4081 ggcaatgcat atacaggaaa ctggaattca tttgtattca gtctgtccat tccggttgct
     4141 atcttttttg ctgcaaggta ttttgtccct ttctaccgga gtatcaacag cccgtcagct
     4201 tacagttttt tagaagagcg ttttggaaaa tgggcccgat tgtatgcttc gagttgttac
     4261 ttgttaacac aaattgcccg tatcggctct gtcctgtttc tactggcgct tccgctcaat
     4321 gccatgctgg gctggagcat cccgttgatt attatcctga caggagtctg cgtagtgtta
     4381 tatgcggctt taggaggaat caaggcgatt gtttataccg atgccataca aggattgatt
     4441 ctgattgccg gggcattggc ctgtctggta atccttatgg tatccatgcc tggaggaggt
     4501 gacgaactgt tgcgtatcgg aatggagtac gataaattct ccctggggag tttgggattg
     4561 actttgaatg agcccacgtt ttgggtaaca ctcatatacg ggctgttcat taatcttcag
     4621 aactacggca ttgatcagaa ttatgtgcaa cggtataaga cggccagaaa tgagaagagc
     4681 gcacgctttt cggccctttt tggcggcctt ttgtatgtac ccgtcagttt attgttcttt
     4741 tttattggca cggcgttgtt cgtttattat aaaacccagc ccggtttgct tccggaaacg
     4801 gttaccggag accaggtatt tccctatttt atcgtgaatg gcttgccggc aggatttacc
     4861 ggcctgttga tcgctgccat atttgctgca ggaatgagta cggtctctac cagcataaat
     4921 agctctgcaa cggttgtatt aaccgatttt tttaatgtga cttctcacag tgaagccgac
     4981 gaaaagagga aaatgaatat cctgcacgcc acatcagtca tcctgggagc attgggagta
     5041 ggtgtgggat tagccatgat gtctgtgaag agcgcgttgg atgcttggtg gagcatggcg
     5101 gcaattttca gcggaggcat gctggggtta ttcttcctgg gatatatagc tcgtgggata
     5161 aaagggtcat atgcttttgt aggagtgatt tgcggcgtat tgctcattgc ttggatgtcg
     5221 ttaagcaaac aaaccgtttt tcacaattat ttaaccattg ttctgggaac actcgttatt
     5281 tttactgtcg gcatgttgct ggcaattgca ggtagcaggc tgaagaaggg taattaggaa
     5341 aagctatcgc agactagatc cgttttaacc gatttgttta gtgaaaacag gaaaaacatg
     5401 agtatcttta ctgaggatta tctgaaaaac aagatgtcaa gttcattcgt ttttagtctt
     5461 gaccaataat aaatttaatt ccggtatgga taaaaaaatt agaatattgg cttttggagc
     5521 cacggccttt tctgctttat atgctcagca gaaacccaat attgtcctca tttacgccga
     5581 cgatatcggt tacggcgatc tttcgtgcta cggggccaca cgcgtgcaaa ccccttatgt
     5641 ggacgcgctc gccaacaacg gcgtgcggtt cagaaacgcc cattcggccg cagccaccag
     5701 cacgccgtcc cggtacggcc tgtttacggg agaatacccg tggcgccgga aaggaacggg
     5761 tattgcagcc ggagatgccg cacttatcat taaaccagac agatacacgt tacccaagat
     5821 gatgaaggaa gccggatacg ccaccggtgc cgtgggcaaa tggcatttgg gaatgggcgc
     5881 cgaaaccggg aaacaaaact ggaacgaaag ggtatctccc gggccggcag aaatcggatt
     5941 tgactattcc tacatcatgg cggcaacggg cgaccgcgta ccgtgcgtgt acatggaaaa
     6001 ccagcgagcc gtggggttag accccaaaga cccgatagaa gtgagttaca ccaaaaactt
     6061 ccccggcgaa cccaccggaa aagacaatcc cgaattgctg acgaaactga aaccgagcca
     6121 cggacacgac atggcagtag taaacggaat ttctcgtatc gggtttatga aaggcgggaa
     6181 gtcggcactt tgggaggatg aaaacatagc cgacagcatc acggtacatg ccattcgatt
     6241 tattgagcgt aataaggata accctttctt tctttatttt gggactaacg atattcacgt
     6301 gcctcgttat ccgcacgggc gttttcgcgg gaagaccgat atgggatatc ggggcgatgc
     6361 tattttacaa ttcgactggt cggtgggtga ggtggttcgt gctctcaaag aagccggagt
     6421 ttacgataat acgcttatta tcatcaccag cgataatggt cctgtggtgg atgacggata
     6481 tcaggatgaa gctgtcgaaa aacttaaaaa tcataaaccg tggggtcctt tccggggtgg
     6541 aaaatacagc acatttgaag cggggacacg cgtgccgttt attgttcact ggacaggaaa
     6601 cgtctgtccg ggagtttcca atgcgttggt atcccagata gatatgctgg gcacccttgc
     6661 cgaacttgcg ggaatagatc ttgccgaagc cgggcagttc gatacacaga aacaccttcc
     6721 cgcctggttg ggcaatgacc ctaaaggaag ggattatatt attgggtctg ccggttcttt
     6781 gacattactg acccgtgatt ggaaatatat agagccgaac aagggaaatg catattctgc
     6841 acataccaat accgagttgg gaaataatcc tgaagaccaa ttgtacaata tcaccattga
     6901 cagaggagaa tacgataatg tggccgtaga aaatccgttg atggtgaaat ttatgaagca
     6961 gattttggaa gaggaaaagg ccaaaggtac aggtctggaa ctttaaactc ttacgtatct
     7021 atagtcttta tctatgatgg gcggtgaaaa atctatcaag aaaatatgtt ttataacata
     7081 tttttctcta tctttgtact gattttaatg gcaagcgagt tattagaaat caattttagg
     7141 ttatcaacat tattctgaaa aaagtttctt gtaaacggtt gtcgataaat cataactttt
     7201 aaagaaagga taaaattatg aacatcaaaa accttacaaa agaagacatt ttgtctcaaa
     7261 tcaagtattt ggaacaaaat atcaacaaag gatctgctgt atatcagact aatcgaatca
     7321 gcaggatcag aacattgaaa tcaagcttgc gtaaccgcag gccagtatct ctgtgatagc
     7381 atcgcgatcg gtaactgatc cgaatgcttt acttcaacta caatttacac cagatattta
     7441 acaaaaagct gctcatccga acagcttttt ttcgttttat ccggcgatca tctcttccgg
     7501 tgaaagattc tccagctttt cctccttttt tttctttagg gctattctca gccaggtata
     7561 cccaattatc atcgataaag tagatgctac cagtactgcc agtttagcgg tgtcttgcag
     7621 ggatttgtcg tcgaatgcca gcatcgatac aaaaattgac atcgtaaaac ctattccggc
     7681 gaggataccc acacctatgt ataacatcca gtttggtttg gttttcagct tgatgattcg
     7741 cgtttttgtc attaaataca cagccaatga aatacctgcc ggctttccga ttaataaacc
     7801 aagaataatc ccgatggata attcgcccaa caaattagct atagccgagc cgttgataaa
     7861 tatgctggta ttggccaacg cgaaaatggg aatgataagg aagttgacag gaaacaaaag
     7921 tactttttca tagaccggaa tcttttttac aggaagcaat gcggatagaa tcacccctgc
     7981 aaaagtagcg tgaatacccg attgatacat acaataccac atcactactc cgagtgaaag
     8041 atataagatt ctggataggg gatttcgtaa atattttatt accagtacga tcagtataat
     8101 acacgctgca actcccaata gccacataaa ctcaggttga cttccgtaaa agaaagcaat
     8161 gatcaggata gcacagaggt catcaatgat ggcgagtgcg gtaagaaaga ttttcatcga
     8221 gtgcgggaca gattttccca ataaacttaa aattcccagg gaaaaagcaa tgtctgttgc
     8281 tgcgggaatg gcccaccccg actgataaat cgttcctttg gtggctgtta agaaaatgat
     8341 tgccgggaaa ataacgccaa acaatgcggc aaccatcggt agcatcattc tacttggtgt
     8401 agagagctcg cctataactg tttcacgctt gatttccatg cccacctgaa agaaaaataa
     8461 cgccattaag gcatcgttta ttatgtgcag gatgctgtgt ggtaaatgta tggagtgtaa
     8521 gaatggaatt tccgtttccc aaaaatggtt gtagctttcg ccgatgccgg gtaaattggt
     8581 taaaatgagt gatgctgttg tgcaaagcag gagcagtacg ccaactgcac gactgtcgtg
     8641 taggaaatct gttatggtaa atttacggct ttttgttcct gtagggtgta gatgctgagt
     8701 cataaatgga ctgtttatga aagtaagaaa ttgaaatcat cttcgtggga cagctccctg
     8761 ggctcttgcc cgaatttaaa aatacgtgcg ttacgttctc caacaagaac cagttccttg
     8821 tcttccagca ataacattgt tccttcgcgc agtccaacga caaaaagatc tctattgact
     8881 tcgataaatt cgttgatgcg cacctcgcgg gtttctccac cgtggttggc cggatgatca
     8941 tccaggtaat gcgggtttat ctgaaacgga accagcttga gcgttttaaa tttatccggc
     9001 tccacaatag gcatgtcatt agtggttttc agcgtgggac aggcaatgtt tgagccagca
     9061 ctccacccga tatacggtgt tcctttcttt acgcgcttcc gaatgacctt catcaatcct
     9121 ttttcctgaa gaattttcac caattgccag gtgttcccac ccccgaccac tattgcgtca
     9181 gcattttcaa tagcctctac gggattgata aaccggtgaa taccggttac gtgatgtccg
     9241 atttcggcaa acctgttgtt caccttttct tcgtattcat cgtaagagaa cgtgacggcc
     9301 gcataaggta taaaaatagc gtttttggct ctgtcgccca ggaaattctt aatgtgctct
     9361 ttgggataat ccaaataagc ttctcctgga ttcgtggagt tgctgattaa caataatctc
     9421 atctttttgt ggttttgaag ttttgtggat gcaaagataa caaaattgtt gaaaatatcc
     9481 ggttttaagg ctatatttgc actcaaaatt cttatcagac aagcagatat gaatgagata
     9541 ttgcaacaga tcagaacgga tttacgccgt tccatgaatg gtatcgcttc taagagtatg
     9601 cgggagaagg gactgcatta caagctgaat tttggagtgg atgttccccg gctgcgtgaa
     9661 ctttccaaaa gatatcccat tgatgctcaa ttggctgaac tcttgtggag acaggaaacc
     9721 cgtgagttaa agatattggc aacgatgttg tatcctgttc atgaattcga tatggataaa
     9781 gccgatgaat gggtgaaaga aattcccaat cacgaaattc gggaacaggt atctatgaat
     9841 ttattccaga aactagactt tgcggacaag ctggtgcaaa aatggacaga ttcgaaagat
     9901 gaagaagtcc ggaccagcgg ttactggttg tttgcccggt tgttgattgt gaagtcgggt
     9961 ttagtcaata agacaaacca aaacgagatt atggaaaaaa tgatttcaga cttgagtgcc
    10021 gggtcctact tccttcgtct ctccgcacaa cgtgcattga cctttctagg caggactacg
    10081 gagtcgtttt caaaaaagat tttggacggg attcaggatc ttcaaacttc gggtgatgct
    10141 gccaaaaaag aaatctttga cagcctgagc ttcgaattta cggattttcc cgattgacta
    10201 aaggcagttt ggatattaaa ctatcaatct cctcctcctt gcaatggata aaaatgtttt
    10261 tgtgaaaatc acaaagttta tcggatgcct gcaaaaacaa ctctttttcc ggtggcgcaa
    10321 gtacgtcgga taacatcgtg gcagacttcc acaacccggg aaaaaccttc gtcagttccg
    10381 ccctcccttt atcggttata aaaacacgca tcctgcgctt gtcttcctcg tcgcggcgct
    10441 caccgataag cttcgctttc agaagtcgcc gcataacttc attgcctgaa gttttttcaa
    10501 tcacattcct gttgttgatc tctgtcttgg acaaactctc ttcattaaac aatgctacca
    10561 ggtaggtgta ttcatcgact gtttggagca gtgatccctt cagggctttc ttgatgtaga
    10621 acttggaata ccggtgcagg aaacttatgt tttgcgcaat acgtaccgtg tcgtttctgt
    10681 tctctttgat tgtccgtctc tgttgcagaa aaagaataaa gtcattcagc gaaagttcta
    10741 cttcggttga ttgagttgtt ttctcaaact gatccagtaa ttcaattatc tcgagcaaca
    10801 atttttttga tttcatacga taatatttat tttttttaca ttaaggtcgt atggaactcg
    10861 cgtattgtca tgttcttggg tgtaactgat ttacatactc atttcataaa cgactaaatt
    10921 tgtacgtaaa agtactaaat cttgtgttaa tatacaaaat atcctgattt aaaatagcat
    10981 atagtacgaa aatgtattat atttgtgctc taataattca gtagagatgg aagttgcagc
    11041 aagaattgaa gaggcgggta aggaatacaa aacgggagac accacgatag tggctttggc
    11101 tccttccact actgtattca ataaaggcga agtaaccctg attgttggtc cttcgggttc
    11161 tggaaaaaca accttgctct cgttgctcgg atgcgtcatt tatcccacaa ccggtaacgt
    11221 ctggctggga gatacgtgtg tgaatcattt atcagaggca gaactggcga aaatccggct
    11281 aaacgaaatc gggttcgtat ttcaaggctt taatcttctg gctccgctca atgctcttga
    11341 gaatgtgatg caaccgcttc tcctcaaagg cgaatcacga aaggtagcca agcaaaaatc
    11401 gctgtcaatc atccggaaat ttgatatgca gtcgcgggta aagaacctgc cccgaaacct
    11461 cagcggtgga caacaacagc gaattgccgt tgcgcgcgcc ttggtttccg atccgaaact
    11521 tattctttgt gacgagccca cggcctcact cgatcacaaa agtgccgtct tggtgatgga
    11581 tatgttaaaa gcgctttcac gcgaagaccg tgccgtgatt attgttaccc acgatacacg
    11641 cttaaagaaa tatgcagacc ggaccattta cgtttcggaa gggaaaataa gcgatacgcc
    11701 gtttgaagaa gaatttgatc attaattaaa aacaagatac aaatgaaaaa actattcatt
    11761 ggctgttttc tcattttgtt gctcgtgggc tgcggtaaca aaaccgaaaa cagggtttcc
    11821 gtagaagtga gtcccgtgac agtggccgtt ggtattggga aaatagtacc acaaggcggc
    11881 gtcagtaaac tggcttcgcc tgttgcaggt attgttgttg aaatagctgt ggcgacaggt
    11941 gacaaggtga aatccggcga tttactgctt acccttgaca ataccgatgc atcgctggcg
    12001 ttgagcgaaa taaacagccg gttagtcacg cagcaaaaat ccatacaatc ggcagagctg
    12061 atgaaagagc aaggcctgac ccggttacgg gaaaaagagc gaaaactgaa cgatgcccgt
    12121 gaactgtttg atgcgggggc ggtaaccggc gaaaatgtgc gtaaccttca aaacgattac
    12181 gatttggaaa aacaaggtca ggagaaactt caaaacgata ttttgttgca ggagtctcag
    12241 cttcgcgaaa ttgtttcgca gaaaaatatg aggtcagaag agttaagccg gacatccttg
    12301 cgtgcaccca tggacggaat tgtactagac gttcttccga aaaaaggcga agccgtgaac
    12361 aggtatgaaa cgtatatgat gctggctcct gacgccccgc ttatcgtaca ggctgagata
    12421 gacgagatgt tctcaaaccg cctagctttg gggcaatcgt gtgagataag ggttgcggga
    12481 aatcctcaac cggtggcaca agggaaaatc ctcagtatct cgcccgattt aaagaagaaa
    12541 tcgctctttt ccgacagcgg acaagatttg caggaccgcc gtgtacgcga gattgaaatt
    12601 tcgatcgaca aagacaccaa tttgcttatc gatactaagg ttgaatgtat gattcattta
    12661 aattgagttg acgtgtttgc aacagcctat aaattcattc gattcgagaa atccaaaagt
    12721 acggggattc tgctggggat tattatcagt atctacctga ttgggctgga gctgggcatt
    12781 ttcttttacc tcacaacact tatcggtggt gtgataaaca acgccaaacc cgaatattct
    12841 caggtctttg tcgtgaacag acttaccaac aatgctaata ttctttcgcc gtttgacaag
    12901 cgttgggtga accagctgag gagtatagaa gggattgacg atgcgcacgg gatggtgatg
    12961 gctaatgtca gtgtccgatt cgataacgga gaaagttcgc cggcaataat tattggaagc
    13021 gagtatcccg aactggctgc aggcccgtcg gaatcactgt tgaactccgg ttccacgaac
    13081 aatctgttcc ttcatggaac ggtttccacc gatttttacg acaacaagac attcggttat
    13141 gacgtaaaac agggcacaag tttcgagatc agcggaaaaa atgttactgt aggggctacg
    13201 acaaaaaatg ccaaaggctt ttcgagtccg atgctttata caaccgatgc cacggcacgt
    13261 tactattccg gcttttcgga agatgcagtc aacgcagtta ttgtgacggt aaaggatgag
    13321 tcgaaaatcg atgaggtcat cgaacggata aataccgttg ctccagacct gcgtgcatgg
    13381 cggtcggaag aactcaaaaa tgctaccgta ataaatgtaa tgacggcgaa caatatgggt
    13441 atgagtttcg gaacgttggt catttttgct atcgtgagtg gattttttat catcgggctg
    13501 acgatgtatt ccgccactta cgaccggatc aaggattacg gaacgttgaa agccattggt
    13561 gctaccggta gacacattac tcttttggtt ataacccagt cgtttattta tgcagttgtg
    13621 gggtttgcgg tttcgttgct tcttttggtc gggacaaaat ttggaatggc taaagccgga
    13681 ctcatcatca atctctcccc tcagttttta gcatttttat ttttcgttac cctgatcatt
    13741 gcggtagcca gctccatgtt ttccatccaa aaactaaaaa aagtagagcc gtcatcggta
    13801 ttcagataaa gatttcaagt tcacaaaaac attacaatga aaaaaacata tatactttct
    13861 cttgcatgga ttgtatccgt tttatccgtt ttgggccagg agaaaaagat aaatctaaat
    13921 gcactgacgc tggaagaggc cgtaaagtac tcgctcgatc attcgccgac gttcatcgcg
    13981 caacagttga aagaacaaca ggccggatat aagctttcgc aagtaaaact ggattacctg
    14041 cctgacattt atgttacatc cgatctgcgg cggaacatta ttattccgtc aacacccatc
    14101 cctgcatcaa tgatcaatcc ttcggcaccg gaagacgagt tgatgtatat gcgtttcaac
    14161 acgccgtgga gttcgggagc ggggctaaat gttacgttcg atatttttaa tcccgaaaca
    14221 gcgggattaa aatcagagca ggagaagcag ttgaaaatca gtcagcttga ttccagaatt
    14281 gctgaaaacg agttgcgagc cactgtttca caggcctata tcgactgtgc gattgcgcag
    14341 acccagctgg acgcaataac tgccgatacg gtttattacg ccgcgttatc acgagagata
    14401 gaagatcttt accggcgtga aaaggtttcg ctggtggaca agaacaatgc ggaaatgaat
    14461 tataacgctt cgctcgcccg cttccatcag gccaaaaata tccagcacaa ctccagaata
    14521 aacctgttga tgtatttggg agtagaaccg gacgaagaca agttggatca gctacggttg
    14581 tcggatgata ttcagtcgct ttattccaaa atgatgtcag gcaaagtgtt aagcggcgaa
    14641 aaccctcttt cacaacgccg ccaggctgag ttggtttcgc tgtctgagat caagacaaaa
    14701 aatgcactat ggaaatatgc gccgtcgttg tcgttatcgg gatattatgg agccaattat
    14761 ttcgggaaag agttgaagtt gggaaacacc aacaggtggt tcgggaacag ttttgtggct
    14821 ctttcgcttc agattcccat ttcgcgatcg ctgagcacgg caaaagaggt ttcccggtta
    14881 cgcatacagg agcagcttga gcgtgaaaat ttgcgggatg taaagaataa ccggttggga
    14941 gaattatcga gagagctggc ccggttggaa acctgtaaaa atacttaccg gttgaaacaa
    15001 tcgaacatga aattgataac ccaaaatata gtggcaaaac aggcacagtt gcaaaaaggc
    15061 tacacgctcg aaagtgaatt tttatcggaa aaattgcgtg aacaaaatgc cttgcaggaa
    15121 tacttgcaag ccgcctacga tctgttgtcg gcttatataa acctggaaaa actgacgaaa
    15181 aactgatccc cgctattttt tatcacgata attgttctgg ataaaaaaca tggatataaa
    15241 aatattcacg atcaggaagc cgcccccgct tgccaggaaa atgcctttcc atttcgggag
    15301 cacaatcagg cagacaatgg aggcaatcgc tatgacaacg ataatcgccc acattgccgt
    15361 gaaaatttgt ttttttgtag ccattcggtt agttatttag ttgttttctt atccaaaccg
    15421 tgatatccga caaaacatcc agcgaaagag tctgttcgat ttttccatac tcatcggcac
    15481 gaccggtttc gcattcttga aaaagatgat tcagcccggg atacgttttt gtttcgtatc
    15541 tgtaatttcg ccccttgtcc aaagccgatt ttatagctcc taaatttttt tcagccagca
    15601 cctgtgtatc tttttcgcca ttaattgcca gcaccggaca ttttaccttt ttcaggtatt
    15661 ccgccggatc caatgcaagg aactggcggt accagggggt ggcgatcgtg ttgaactggt
    15721 cgcggataaa agcatccttt ttgagtttca ttttaacgag aatagggtac tgttcccaaa
    15781 gttgcgaaag ctggtcacgc aatgctaccc ggtctgcttc tgtccctttc cattccgaaa
    15841 ggctttcaag catctggcgt atcaccttta atgactgatc gctgttttcg ggttctatat
    15901 tgagcagttc cagtgccata cggttttgct ccattatggt ttcaatccct cgttctccca
    15961 ttcctgccat aaggacgata aatttgatgt cccggttttt cgatgccacc atgggcgcga
    16021 tcacaccgcc ttcgctgtgt ccaataaggc caatggccga tttatctacc tcttctcgtg
    16081 ccttcaaata ttccacggca gcagcaacat ccgtcgcaaa atcttgcgta gtggctattg
    16141 ggtagtaacc tgttgaccgt cctgtgccac gtttgtcgta cctgagtacg acgaatcctt
    16201 ttcgcgtcag atgatcggca atcaccagga acggtttgtg cccgaaaatg gtttcgtccc
    16261 tgtcgtgtgg tccgctgcct gcaatcagta tcactgccgg gaatgttcct gcagaatcgg
    16321 ggacagagag tgtcccggca agttcaatgc tgtctttttt atggatgaat ttcacctctt
    16381 ccgttttgta cggaaatggg ggttggggtt cctgtgggcg gtttaggacc ggtttgtcgg
    16441 tcagcttgag aaccaaaggg aagggtatcc cgccctgatt gaacgttccc aatatggaat
    16501 ctccctctaa tgttccctga taaaaaatgc ccaatcctgt tgctacaatc tcgagtttat
    16561 tttcaacaaa agaagtcttt gaagtgggca accctaatgc gttctgtgcg gggctatcca
    16621 tggttgtgga gtaagaactg tcggttttag agacgtggaa aacgacgggc agcttgtttc
    16681 ccataacgtt tagcgttccg ctccagcttc cggtaatttc ctgcgcggaa acggaaacag
    16741 acactaaaaa caagaaaagg attattgcct tctttttcat gaaacattct ccattattcg
    16801 tgatgatacg gttcaccatt caaaatagtc attgccctgt agagttgttc cacaaacacc
    16861 agtcgaacca tttggtgcgt aaaagtcatt ctcgataacg acaacttttc gttggcacgc
    16921 ctgtacactt cgtccggaaa tccataaggc cccccaacaa caaaaacaag ccgtttaggg
    16981 acggaatgcg acttcttctc aatatagcgg gaaaattcca cagaagagta ttgtttccct
    17041 ttgtcatcca gcagaacgac ataatcgccc tgttgaatcc ggctcagcat ggcttcccct
    17101 tcgaggttct tttgctcttt ttccgataag ttttttgtgt ttttcacatc cgggacaaat
    17161 tccagatcga aaggaaggta atgacttaat cttttcctga atatatccat cgcctgaacg
    17221 taaaaatcct catcggtttt tccaagggac agcaaaacaa ccttcatctt ttattaatac
    17281 atttttttta cgtaaaatta gcaaccagag tgcaaatata accttttatt tttatatttt
    17341 tgcattcaca atttacaagt tttcttaaca atacgggatg aattttgtta ttgttagtga
    17401 gcaacccaat ttagcttgaa tggaattaaa aaaattgacg gacaaactcg ttgatctggc
    17461 ttttgccgag gatattggcg acggcgatca taccacactc tgcagcattc ccgaaacagc
    17521 tttcgggaga gcccggcttt tgattaagga agaaggtatc ctggcaggag tggaagttgc
    17581 caggacgatt tttcatcgtt tcgacgaagg attagtcgtg gaggttctta ttaatgacgg
    17641 aacatacgtt aagcccggag atgttgcatt cattgtatcg ggaaaagtcc aatcgttgct
    17701 gcaaaccgaa cggttggtac tcaacattat gcagcgtatg agcgggatag ccactactac
    17761 agggaaatat gtgaaattac ttgaaggtac caaaaccaaa gtgcttgaca cgcgcaagac
    17821 tacacccggg atgcgaatgc tggaaaagca ggccgtaaaa attggcggag gggagaatca
    17881 ccgtatcggt ctgttcgata tgattttgct gaaggacaat catgtggatt ttgcaggcgg
    17941 tatagaaaac gccattcgtg gagcacaaaa atatctgaag gaaaaaaaca aacaactcaa
    18001 catagaaatt gaagtacgta acttcgatga gcttaaccaa gcccttgcgg tgggaggggt
    18061 agataggatc atgttggata atttcacgcc cgaacaaacg tgtgaagcgg tgaagattgt
    18121 ggacgggcgc gttgaactgg aatcgtcggg cggaattacc tttgaaacta tccgtcaata
    18181 tgcggagtgt ggtgtggatt atatctctgt aggcgcgctt acccattcgg taaaaagctt
    18241 ggatatgagt ttaaaagcga tagaatagca gttagcaaag tataatgaca gatacaaaac
    18301 agctcgaact ggacaagcga tacatgcgga tggcatttat ctggtcagaa aattcatact
    18361 gtaaacgccg taaggtaggt gccattttgg taaaagacaa gatgataata tccgatggat
    18421 acaacggaac accatcggga tttgaaaatg tttgtgaaga tgaggataac gtaacaaagc
    18481 cttatgtgct tcatgccgag gcaaatgcca taaccaaagt ggcccggtcc aacaacagta
    18541 gtgaaggagc aacgttgtac gtgacgtctt ctccctgtat tgaatgtgca aagctgatta
    18601 ttcaggcagg tatccggaaa gtcgtttatg ccgattctta cagactgagc gatggtgtag
    18661 aattacttag aagggctgat atagagttga tttcggttga tttgtgaaaa attaagttgc
    18721 aagaataaga taatgagtcc tgaaaaaaaa ataaaaacgt ggcttccttt atgggttgga
    18781 ttgggtattg ctttgggaat tcttatagga agcatctatt cgcaatttgg gaatacggga
    18841 aaagtggatg gaaccggtaa gattgatgcc atatttaact atatcaacaa atcgtatgta
    18901 gacaccgtga atattcgtca gcttgtggag gaggcgctac ccaaaattgt tcaggaactc
    18961 gatccacatt cggcttatat ctctgccagc gaaatgaagc gtttaaacga agatctggag
    19021 ggccattttt cggggattgg tgttagtttt tatgtattaa gcgatacgat cgtagtgaca
    19081 agtattgtgc ctggcggtcc atcggaggcg gcaggtatac agcaatggga caggatagtc
    19141 aatgtcaatg acacgctgat tgcagggcgg aaaattacaa acgccgatgt actgcaaaag
    19201 cttcgtggtg aaaaaggatc cgaagtaaaa ctgggaatta aaagaaacga tgatacaaaa
    19261 ctgattgaca taaccgtaac tcgtggagac attcccatga acagtgttca ggctgcttat
    19321 atgcaaacca acaccatcgg atatatcaag gtgggcacat tcggattcaa cactttcaat
    19381 gagtttatat cggcactgtc taagctcaaa agccaggggg cacattcttt cgtgatcgat
    19441 ctgagaggta acagcggtgg ttcgttggaa attgttgttg ccatggtgaa tgaatttctt
    19501 cataaagggg atttgattgt ttatgctgac ggacggaact ttcccaggca ggacagttat
    19561 gccaatggtt cgggaacgag taaagaagac gatgtcgtca ttttgattga tgaattaagt
    19621 gcatccgcaa gtgaaatttt tgccggtgct attcaggata atgatcgagg attggtgatg
    19681 gggcggaggt cttttggaaa aggattggta caaagccagc gaaattttcc cgatggatcc
    19741 gctgtgcgtc ttaccgttgc ccgttattat acaccgtctg gacgttctat tcaacgcaaa
    19801 tatgaaaaag gaaagtacga tgaatacgag ctggaagcat tgaacagata tatgcaaggc
    19861 gattacgtaa attccgatac agcgtctacc ctcattccct ttaaaactga aggagggcga
    19921 acagttttcg gaggtgacgg tataatgccg gatgtcttta ttgcccgtga cacaatcggc
    19981 atgaattctt attttaatac gatcgcaaac aaggatttca ttcaagagta tgctgtgacc
    20041 tattcagata tgcataagca taaattgagt gaatttcaat cagcagaaga ccttgctcgt
    20101 cacctgtaca agcagcctct gctgatgaat ctagtaagtt acgctgacaa caaaggagtc
    20161 cggccaagac cctattatat agaagaatcc aggaagctgt tcgagcgaca tttagttgcc
    20221 tatattgtta ggaacttctt cggtgaggaa agctatttct ccgtatacat gcaggacgat
    20281 gtgttgatga aaaatgcagt ggattttatt gagagagggt tggcgaagcc cgaatctgtg
    20341 gtacaagaaa agtataagtc catttcgctg ggcctgaggg tcagctttcc tgttcccaag
    20401 gaatttggct cgttatttta tgtatagaag aaatgaatat aacagagcag aaaaaaagac
    20461 tccgtgaaag gattgcccag aaaaagcaat cgtattctca aagagaactg ttaaacttct
    20521 cggaagaggt tatatcaaca cttgaactca ctgaggtatt tcagaacgct aaagttgtac
    20581 tagcctatta cagcatgtcg gatgaagtta atacgcatga attgataaaa aaatactgtt
    20641 tacaaaaaca ttttcttctg ccggtggtta ataatggtga attaattttg aaaaaatatg
    20701 tctccgaaga aagtatgtcg acttccgctt atggaattaa agaaccggtg ggcgatactt
    20761 tcccggaatc tgaatacggt aaaatagatt tggtaattgt cccgggtgtg gcttttgatc
    20821 gcacactaaa ccggttgggt aggggaaaag gcttttacga cagattattg ccaaagataa
    20881 aggctcctaa gacagctata tgctttgatt tccaggtttt tgataatatt cctgtaagtc
    20941 agggagatat taaaatgaat atgattgttt gtcagaatga gattattgtg gaataactac
    21001 tcagcaattt gaaattcaga taaacatttt ctttaacatc acaattcttc ataaacttgc
    21061 agtatattta atgattttaa atgtgctgtc gttgatcttt gagtgcagtt atgaataaat
    21121 tcttagagaa ttacttatcc aacagggtct tgtcccgcat gtctatcctg ataatagaca
    21181 ttcttattat actgttttca accctgacca tgtatttttt gcgtttcggt tttgaaggac
    21241 ttacgacgca ggtcaaggca gatggtataa ctacaacact cgttctggta tttttcaatg
    21301 tcatttcgtt cctaatcttc aaaactttta gcggggtttt acgcttttcg gctttttccg
    21361 atcttgttcg gatcatatat gcattgactc tgggctattt tatatcgttt atctgcctga
    21421 tggtgataaa aaaagtcgat cccactttca ctgtttccaa taccattcat tttgccactt
    21481 atgtgttgaa tgttttgctg atgatttttt cgcgtattgt tgtcaaggag gtgtatgagt
    21541 ccataactgg aaagccgcag aaagctacca atatttttat ttacggcacc aaacaggccg
    21601 gtattagtct cgcaaaagcg atcaggggaa accgggaatt caagtacaaa gtattgggat
    21661 ttattaccga tgaagacaat atggtcggta aaaatctatt ggggcttact atttatgcca
    21721 acaacgaaaa cttattcagg gtactggaag caaaagacgt aaaaacagtg attgtctccc
    21781 ctcgtaaaat ggatcagata agaaattcgc ctttactgga aaatttcgtg gaccataata
    21841 tttcgttgct gactacttct cccgttaacg aatggaacgg gacattgaca ggaaaagagc
    21901 agctaaaaga tattcagatt gaggatctgc tcccccgcaa tcccattaac atcaacttaa
    21961 tggaaattgc tgctcacatc gaaggaaagc gggtgatggt aacaggggca gcgggttcca
    22021 tcgggagcga gattgtccgt caggtagcct cattcaatcc tttcaacatc attttgatcg
    22081 atcaggcaga aacccctcta catgacatga ggcttgaact gcaaaaaaaa tggatcgata
    22141 taaataccaa cataattgtt gctgatgtag gcaatgctac aagaatggag cgcatttttg
    22201 caaagacgcg cccgcagtac attttccatg cggcagctta caaacatgtc cctatgatgg
    22261 aggacaatgt ttctgaatcc atccagacaa acgtcctggg gacaaaaatt ctggccgacc
    22321 tggctgtaaa atacggggct cagaaatttg tcatgatctc tactgataaa gcggtgaacc
    22381 cttcaaatgt aatgggttgc tcaaaaagaa ttagtgagat ctatgttcaa tccttatcga
    22441 ggcatttgga gaagaacggc cagaagacca cgcaattcat cactacccga ttcggtaatg
    22501 tattggggtc aaacggttcg gtaatcccgt tgttcagaga acaaattaaa aatggaggcc
    22561 ctttaacggt tacacatccc gaaattattc gttatttcat gaccatcccc gaagcttgcc
    22621 agttggttct tgaagctggt tccatgggta aaggaggtga gatttacctt ttcgatatgg
    22681 gaaaaccggt taagattctg gatctggcaa aacggatgat ccggttgtcg ggctctcaaa
    22741 acataaagat agaatttaca ggccttcgtc acggtgaaaa gttatacgag gaattgctca
    22801 atgcctctga aaacaccatt aaaactcatc acgaaaagat tatgattgct cgggtgcgtg
    22861 agtatgagta cgaaaaagta aaggatcaga tcgagggatt aatcgagatt tcgtatcaat
    22921 acgacgatat gcgtactgtg aaaaaaatga aagaaattgt cccggagttt caaagcataa
    22981 attcccctta cgaggctgtt gaccgcttac tggagaaact ggaagacaag gaaagcgtta
    23041 agatacagga tgctttttca atttagatct ccctgcatat tgcaaagttt tgtataatag
    23101 ccgtttaact catacaacga attgtgtccg cctgattcga tgatttgtcc ctcctttaga
    23161 acgtaaattt cgtctgcatt ttttatggtt gacaaccggt gcgcaataac aattgttgtc
    23221 cggtttctca tcagtttctc cagggcatcc tgtaccaatc gctccgaatc ggtatccagt
    23281 gccgaggtag cctcatccag tatcaggatt tccgggttct tcaacaccgc tcgcgcaatg
    23341 cttattcgtt ggcgttgtcc tcccgatagc tttcctcccc gatcaccgat gtttgtctga
    23401 tatccgtgtt gagtggccat gatgaactcg tgcgcattgg ccacttttgc cgcttcaata
    23461 acctcttccg aagttacttc ttttaagcca aaggcaatgt tgttgaagaa cgtatcgtta
    23521 aaaagaatgg cttcctgggt cacatagcct atttttgagc gcaggtcgtg taaagtaaca
    23581 tcctttatgt tgatattatc aataaaaacc cccccctcct gaatatcgta gaaccgggat
    23641 aaaagatcgg tcatggtaga cttcccggaa cccgattgac ccacaagcgc aaccgttttc
    23701 cctttttcaa tctttaaatc gatccctttc aatacccact ctttgttgta tttgaaccat
    23761 acattgcggt aatgtatgtt atttttaaaa ctcagctttt gcgggtgctg tatttctgtt
    23821 atgtcgttct gggcctccag tatcttgtcg atacgctcca tggaagcaag gcctctttgt
    23881 acggcatatg ccgatctgga aaactctttg atgggattga taatactgta gaaaatggtg
    23941 aggtaataaa taaaagtggc cgcatcaata atcccgttcc cgcttaaaat aagtgagccg
    24001 ccaaaccaaa ggacaatggc gatggtaagg gttcccagga attcactcat cggatgtgca
    24061 agatactgcc gtcgcgcaat cctattggac atctttctaa acgtatcact ccccgtatag
    24121 aatcgtttgg agattttgct ttccgcatta aacgctttta ttattcttag accgctcaag
    24181 gtttcttcaa tttgcgacat aagttctccc catttgtttt gtgcctcgaa cgaacttctt
    24241 ttcagtgatt tgccaacggt tcccatcaca tatcccatta tcggcaatac cacaaaaaca
    24301 aaaagggtaa gctgccagct gagaacgatc atggtaatca aataaatcag aataagaatg
    24361 gggttcttaa agagcatatc aagcgagctc atcaccgagt tttccacttc gttaacgtcc
    24421 cctgaaatgc gggccaatat atcgcctttg cgttcttctg aaaaaaaacc gatgggaaga
    24481 accagaatct tatcgttaat tttgttccgg atgtctttaa ctactcctgt ccggatggga
    24541 atggtaaaat aagatccgaa ataggctgta ccggttttaa aaagagtcat aacgataagc
    24601 gcaatcgcca aaaaaaggag cgttgtgctg cctccatggg tttcaatgag ccgggccatg
    24661 taccaattcc cgttattcag gacaatatcg atcaatgaaa caccttttgt ttcccaggga
    24721 ataaattcaa aaaccttatc gttgacttta aataaaacct gaagtatagg aataatcgtt
    24781 gccagtgaga aaacattcaa taatgcggtg agtagattaa aaagaaagga gagcaccaca
    24841 tatttcttgt agggaggcaa gaagcgcttc aatatagtta taataccgtt cattaaaaaa
    24901 tatccttatt gcagtagttt tagtgtaaca cgaaaaacgg tgcaagatac acataatttg
    24961 tgattcccgg tgtcagattt cgcgtttttg caaaacggtc tgccatgaaa acagaaaagt
    25021 ggaaaaaaat tcaaaaaaaa atgaattaaa taattcttta attgtcaggc cattgttttg
    25081 ggttttgtaa aatagttgat ttttttatca aatttgttgt gaaaatgttt tgatggaatg
    25141 aaatctttcc ctacctttgc atccgctttc cgaaaacaag tagatgtaga aagcaattga
    25201 tcgtt
//
LOCUS       DAOSDR010000002        22814 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrimonas sp. isolate AMR_MDS_1826
            NODE_998_length_22814_cov_4.591414, whole genome shotgun sequence.
ACCESSION   DAOSDR010000002 DAOSDR010000000
VERSION     DAOSDR010000002.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863355
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrimonas sp. (wastewater metagenome)
  ORGANISM  Petrimonas sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Dysgonomonadaceae; Petrimonas.
REFERENCE   1  (bases 1 to 22814)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 22814)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:10:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,720
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 35
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22814
                     /organism="Petrimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_998_length_22814_cov_4.591414"
                     /isolate="AMR_MDS_1826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2023866"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2104)
                     /gene="ileS"
                     /locus_tag="PLI30_00115"
     CDS             complement(<1..2104)
                     /gene="ileS"
                     /locus_tag="PLI30_00115"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006842934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HOI77989.1"
                     /translation="MSKKFTEYSQLNLSEINKEMLRQWDEQYVFRKSLEIREGHPEFV
                     FYEGPPSANGMPGIHHVIARSIKDIFCRYKTMKGFLVNRKAGWDTHGLPVELGVEKTL
                     GITKEDIGKTISVEEYNNACRTEVMKYTKEWEDLTRKMGYWVDMNDPYITYDNRYIET
                     LWYLLKEIYKKGYLYKGYTIQPYSPAAGTGLSTHELNQPGCYRDVKDTTCTAQFKVIH
                     PLPEWKQFGEVFFLAWTTTPWTLPSNMLLAVGPSIEYVAVQTFNQYTGSPVTVVLAKN
                     LLDSYFPSKNAQLKLEDYQEGDKNIPFRVLEKTWKGTELAGLEYEQLFPWIKVTEKAF
                     KVVCGDFVTTEDGTGIVHIAPTFGADDAKVGKENDVPGLTVVDKDGNTRPMVDLTGKF
                     FRLEDLDGGFVQNNVNVDLYKEFAGRYVKNEYDQALSADEVTLDIDLSVSLKLRNRAF
                     RIEKFVHSYPHCWRTDKPVLYYPLDSWFIRSTACREKMMELNDTINWKPQSTGTGRFG
                     KWLENLQDWNLSRSRYWGTPLPIWRTEDGREEKCIGSVKELCNEMQKALDAGVMSELP
                     WKDFDLKEYRDLEYGKIDLHRPYVDNIVLVSETGKPMHRELDLIDVWFDSGAMPFAQF
                     FYPHITEEEFAKVYPADFIAEGVDQTRGWFFTLHAISTMVKGSVAFKNVISNGLVLDK
                     NGNKMSKRLGNAVDPFEAI"
     gene            complement(2263..3741)
                     /gene="guaB"
                     /locus_tag="PLI30_00120"
     CDS             complement(2263..3741)
                     /gene="guaB"
                     /locus_tag="PLI30_00120"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019539335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HOI77990.1"
                     /translation="MSFIADKITMEGLTFDDLLLVPGYSEVLPRNVDLSTHFSRNIPL
                     NIPMVSAAMDTVTETTMAIAIAREGGMGVIHKNMTVDDQAKQVRAVKRAENGMILNPI
                     SIHPEKTVADALEMMAEYKIGGIPVVIEDNTLVGIVTNRDLRFERNMDKLIREVMTRE
                     NLITTRQTTDLEAAAEILQQHKIEKLPVVDSNYRLVGLITYKDITKAKDKPYASKDEQ
                     GRLRVAAGIGVTFDSVERAAALIDAGVDAIVIDTAHGHSKGVGDMLKLIKKTFPEIDV
                     VVGNVATADAAKFLVKAGADGVKVGIGPGSICTTRVIAGVGVPQLSAIYDVAQALKGS
                     GVPLIADGGLRYSGDIVKALAAGGSSVMMGSLLAGTEESPGETIIFNGRKFKTYRGMG
                     SLSAMQKGSKDRYFQDVEDDIKKLVPEGIEARVPFKGTLQEVIYQMVGGLRAGMGYCG
                     ASTIEKLHEAKFTRITSAGYAESHPHGVMITREAPNYSRDKE"
     gene            3966..4598
                     /locus_tag="PLI30_00125"
     CDS             3966..4598
                     /locus_tag="PLI30_00125"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HOI77991.1"
                     /translation="MSVLVGKRAPVFWAPAVLDGNIIEDNFSLDNYLGKKYVVFFFYP
                     ADFTFVCPTELHAFQEKIAAFENRDTVVVACSTDSAVSHWKWLQTPKSDGGIQGVTYP
                     IVADHALTISMAYDVLAGEYDTDSSDNPIFVGAPEAYRGLFLIDKAGIVRHQVVNDMP
                     LGRSVDEVLRVIDALQFTEEHGEVCPADWHKGDKGLTATQDGIASYLSEK"
     gene            4870..6063
                     /locus_tag="PLI30_00130"
     CDS             4870..6063
                     /locus_tag="PLI30_00130"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011108289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HOI77992.1"
                     /translation="MNPLSARLQSLSPSETFAMAQKSNELKAQGIDVINMSVGEPDFS
                     TPDHIKEAAKKAIDNNFSFYSPVPGFPELRKAICAKLKNENNLDVDPTQIVVSSGAKQ
                     SLCNVILSIVDKGEEVIIPAPYWVSYPEMVKIAEGKPVFVYAGIEQDFKITAEQLEKS
                     ITPKTRALILCSPSNPTGSVYTRDELKSLAAVLAKHPGIYVISDEIYEHINYVGRHES
                     IAQFEEVRERVIVVNGVSKGYAMTGWRIGWIAAPKWIASACGLLQGQYTSGASSISQK
                     AAEAAYTGNQDCVEEMRVAFERRKNLITKLLKEIPALKVNDPKGAFYVFVECSSYLGK
                     QFDEKRIDTATDLAMYLLEQGHVACVGGDAFGAPGHIRFSYATSDENIEKAVSRVKGA
                     LEKLR"
     gene            complement(6145..6996)
                     /locus_tag="PLI30_00135"
     CDS             complement(6145..6996)
                     /locus_tag="PLI30_00135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008773101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HOI77993.1"
                     /translation="MKRLPFFFLLFFSIILIGCSTTKKSLTMNIATFNVLMDTKKDHP
                     NAWKYRKEMVKGLVRFHDFDIVGTQEAFKHQLTDVLELGNYAYTGSGRDDGKDAGEHS
                     AIVYKKERFDLLENGDFWFAENPDVPGKGWDATCCNRICSWAKFKDKETGKVFFVFNS
                     HYDHEGREARRNSSLLLLKKIEEIAGDNTVFCTGDFNATPDDEPIQILRNDGRLKDSY
                     LISVQPPYGTEGTFNALNLNSPMKGRIDYIWVTEGVKVNKYGVLNELPYARFPSDHFP
                     VMINVTF"
     gene            7424..8110
                     /locus_tag="PLI30_00140"
     CDS             7424..8110
                     /locus_tag="PLI30_00140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005859976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase cytochrome b subunit"
                     /protein_id="HOI77994.1"
                     /translation="MSWLIKSSIGRKLIMSISGLFLVLFLMFHSLMNFVVILSADAYN
                     TIASLLGANWYALIATGILALGFIIHIIYASILTLQNQKARGSNKYAVSQPQKNVSWA
                     SKNMFVLGTIILGFLVLHLIHFWSKMQLVELMHGHNYAAAGYHDPTDGAYFIRELFTQ
                     PLYSIVYIVWLVALWYHLTHGFWSAMQTLGWNNQIWLPRLKKISYVVATVICLLFISV
                     PVYYLLGFGA"
     gene            8138..10141
                     /locus_tag="PLI30_00145"
     CDS             8138..10141
                     /locus_tag="PLI30_00145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018028096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase/succinate dehydrogenase
                     flavoprotein subunit"
                     /protein_id="HOI77995.1"
                     /translation="MINKEDETAKVENKTTLTDNKIKLDSKIPKGPLAEKWSSYKANQ
                     KLVNPANKRRLDIIVVGTGLAGASAAASLGELGFNVLNFCIQDSPRRAHSIAAQGGIN
                     AAKNYQNDGDSVYRLFYDTIKGGDYRAREANVYRLAEVSNNIIDQCVAQGVPFAREYG
                     GLLDNRSFGGAQVSRTFYARGQTGQQLLLGAYSALSRAVNKKQVKLYTRYEMVDLVII
                     DGRARGIIARNLVTGKLERFAAHAVVIATGGYGNTFFLSTNAMTSNGSAAWQCYKKGA
                     YFANPCMAQIHPTCIPVHGEFQSKLTLMSESLRNDGRIWVPRKLEDAQAIREGKLRPT
                     EIKEEDRDYYLERRYPAFGNLVPRDVASRAAKERCDAGYGVGTTGLAVYLDFADAIKR
                     LGKDVVEARYGNLFQMYEKIVDDNPYETPMMIYPAIHYTMGGIWVDYELMTSIPGLFA
                     IGEANFSDHGANRLGASALMQGLADGYFVLPYTIQSYLSDQIRVPRFSTDSPEFEKAE
                     KDIDARMERLMSIKGNHSVDHFHKKLGHIMWDLVGMGREADGLKKAIEELKVLKKEFW
                     ADVRIPGEKSSLNIELEKALRLADFIEIGELMAHDALDREESCGGHFRLEHQTEEGEA
                     LRHDDRFSYVSCWEYQGEEKAPVMYKEPLDYEFIVRQQRNYKS"
     gene            10162..10917
                     /locus_tag="PLI30_00150"
     CDS             10162..10917
                     /locus_tag="PLI30_00150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004584033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase/fumarate reductase
                     iron-sulfur subunit"
                     /protein_id="HOI77996.1"
                     /translation="MDKNININVKVWRQKGPKAKGNFETYALKEISQGSSFLEMMDIL
                     NEQLINEGKDPVVFDHDCREGICGMCSLYINGHPHGPDEDITTCQLHMRKFNDGDTIT
                     VEPWRSAGFPVIRDLMVDRTAFDKIIQAGGYVSVNTGGVPDGNAIPIPKHAADEAMDA
                     AACIGCGACVATCKNGSAMLFVSAKVSQLALLPQGQVERVKRAKAMVAKMDELGFGNC
                     TNTGACEQECPKNISISHIARLNREFLKAKIKD"
     gene            11101..11724
                     /locus_tag="PLI30_00155"
     CDS             11101..11724
                     /locus_tag="PLI30_00155"
                     /inference="COORDINATES: protein motif:HMM:NF024895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin family protein"
                     /protein_id="HOI77997.1"
                     /translation="MKTKKLWMILLAFLLMGGTVSAQLRFGIRGEVGINKPSFTKDVY
                     SVENMNDFKVGPTLEFMLPVVDFGIEGSVLYSNEKMNIKTVNDQGIKTVAEDISNHSI
                     DVPVNLKYKIGLISPVKIFLAAGPYANFWLAGDDFKSEVFMDKVEAKKFQAGVNLGAG
                     LEVINHVAVGFNYRMKLTDDYSVYEPAFEDIFNQKDGFWSITATLFF"
     gene            11732..13969
                     /gene="priA"
                     /locus_tag="PLI30_00160"
     CDS             11732..13969
                     /gene="priA"
                     /locus_tag="PLI30_00160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004350851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005658 - alpha DNA polymerase:primase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HOI77998.1"
                     /translation="MLFADVILPLPLAESFTYVVPAEMQEKIGTGFRVVVPFGKRKYY
                     TAVVLRLHHTPPKGVEIKEIHSLTDSHPVVNEHQIRLWEWISFYYLSPLGDVYKAALP
                     SIMKPQDLEERYRSRTETYIRLNTGIHDIDRLTGRSKKQAELYQALRQLFLEEGRESI
                     PKKEITAKTGYSNTVLKGLTEKNIVIQYPEEVSRIDTHFVSSRKAFNLNDHQQKALEE
                     INRSFEEKNVCLLHGVTSSGKTEIYIHLIEEQLNQGKQTLYLVPEIALTTQLTSRLQA
                     VFGDKLGIYHSKINDNERTEIWQKMLSDSPYEIVIGVRSSLFVPFRQLGLVIVDEEHE
                     TSYKQQEPAPRYHARDTAIVLAQTFGAKTLLGSATPSIETYYNAKTGKYGLVTLEKRF
                     ENIELPRITLENTRELRRTKKMKTLLAPGLIEKINTALGSGEQVILFRNRRGFAPMLE
                     CKLCGWIPKCNHCDVSLTYHKNRGELKCHYCNKTYKPVGECPVCHEKSIEPVGMGTEK
                     LEEEVIQLFPSATVARMDTDTTQGKYAYEKIISDFQKNKVQILVGTQMLSKGLDFENV
                     SLVGIVSADGLLNRPDFRSHERGFQLMLQAAGRAGRKNRQGEVVIQTADPGQPIYRYL
                     ETNDYEGFYHSQLEERKLFNYPPYKRLISIVFKHKDEHKVESGSAFFARLLQQSLGEM
                     VLGPNKPFISRIQQYHLRQILLKLDNHLSPAKTRQFIKSVESRFREKQDFKYLVLYFD
                     VDVMG"
     gene            complement(14032..15600)
                     /locus_tag="PLI30_00165"
     CDS             complement(14032..15600)
                     /locus_tag="PLI30_00165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005681476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rne/Rng family ribonuclease"
                     /protein_id="HOI77999.1"
                     /translation="MASELVVDVQPKEITLAVLDNKKLVELQQESQSESYAVGNIYLG
                     RVKKLMPALNAAFVDVGHKKDAFLHYLDLGVEFNSVSSFLKTIEDKKKIPLLQKMKLQ
                     PEIEKDGSISDKLKVGQEVLVQVAKEPISTKGPRLTAEISFAGRFMVLIPFIDRVSVS
                     QKIKSREERARLRQLVQSIKPKNFGVIIRTNAEEKRVAELDAELKVLVKRWEDTAEKL
                     PKAKAPSLVFEETGRTVGLLRDIFSPSFEQIHINDQDVAKQIAEYVGLIAPDRKNVVK
                     HYNGAAPILDNFGITKQIKSLFGKTVTFKNGAYLIIEHTEALHVIDVNSGNRNKQSLG
                     QEDSAFEVNVAAAEEIARQLRLRDMGGIIVVDFIDMSSGDHRNKLFNKMNEFLASDRA
                     KHKILPLSKFGLMQITRQRVRPEMELATSEVCPTCFGKGKIKSSILFTDTLEGKIDYL
                     VNKLKVKKFSLHIHPYVAAYIQQGLFSLKYKWKKKYNVALKVIPNQSLGFLQYVFYDK
                     ENNEIDLKEEIEMK"
     gene            complement(15898..16185)
                     /locus_tag="PLI30_00170"
     CDS             complement(15898..16185)
                     /locus_tag="PLI30_00170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006744256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HOI78000.1"
                     /translation="MTKADIVNEIAKNTGVDKASVLATVESFMEVVKDSLANKENVYL
                     RGFGSFIVKKRAEKTARNISKNTTIIIPAHNIPAFKPAKTFVAQVKDNTNK"
     gene            16360..17436
                     /gene="mutY"
                     /locus_tag="PLI30_00175"
     CDS             16360..17436
                     /gene="mutY"
                     /locus_tag="PLI30_00175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019538448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HOI78001.1"
                     /translation="MRKKESDKLISNILQEWYECHRRILPWRDSKDPYVVWVSEVILQ
                     QTRVSQGYDYFLRFMEKFPDIKSLARAQEDDVLKAWQGLGYYSRARNLHAAARQVMEK
                     FDGVFPSSHNDILLLKGVGEYTAAAVASIVFGLPYPVVDGNVYRVLSRLFAIEEPIDS
                     TSGKKLFTETATSILDQQDPGNHNQAIMELGALICTPRLPKCQDCPLQSVCLAFERKN
                     MSDFPVKKGKTIPKIRYFNYFHIEQNGYTYIQKREHSDIWKNLYEFPLIETEKQSDLF
                     MLQDHPGLSTLFQAADEIFLQHKLRLRHVLSHQIILADFYRVEFSKQEMSGLEKKFIK
                     IPATSISEYPVSRLIHKYLETIYR"
     gene            17509..17949
                     /gene="ssb"
                     /locus_tag="PLI30_00180"
     CDS             17509..17949
                     /gene="ssb"
                     /locus_tag="PLI30_00180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019538447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HOI78002.1"
                     /translation="MSVNKVILVGNVGKDPEIKYFDNDNVKANFPLATSERGYTAANG
                     TQVPERTEWHNIVCWRGLAQIVEKFVKKGTQLYIEGKIRSRSYDDQNGVKRYITEIYA
                     DNVEMLNRRAGAGEGAGFQNDKHDTSYNDPVDLNDNSEADDLPF"
     gene            18035..19363
                     /gene="gldE"
                     /locus_tag="PLI30_00185"
     CDS             18035..19363
                     /gene="gldE"
                     /locus_tag="PLI30_00185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010800327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated protein GldE"
                     /protein_id="HOI78003.1"
                     /translation="MDPDPLSLIYPEVQAAALSAADYLLIVLTLTLTFINSIISGAEI
                     AFFSLNQEETEGINTDADPASVRVSHLLQNPERLLSAIVISYNALNITIVTLLFYLLN
                     LIPYFSGSNNKIILEFLFIIAFILLFVEIIPKTYASQRSLAFARSHSRFIQILNKLMN
                     PFSSFLVRTSHIFSKSVIRKRYEISMDDLSKALEITSGEITQEQEKDMLEGIIRFKDK
                     TVEDILVSRSDMVAIDINTSFRDVIHFIVEAGFSRIPVFEENPDNIRGILYVKDLLPH
                     LIKTENFRWQSLIRPAYFVPGTKRIDDLLEEFRANKNHMAIVVDEYGGTTGIVTMEDI
                     LEEIVGDISDEYDEEAKPFYTIAQDGSYIFEGKTSLEDFIEVTGVPEKDFEKLLEDAD
                     TIAGLMLELKGDFPKRKELVEYKKYGFQAEEMSKRRIMKVRFIPPKGDSD"
     gene            19368..20000
                     /locus_tag="PLI30_00190"
     CDS             19368..20000
                     /locus_tag="PLI30_00190"
                     /inference="COORDINATES: protein motif:HMM:NF013786.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase superfamily
                     protein"
                     /protein_id="HOI78004.1"
                     /translation="MLLRKEHIDGDGLIGIGKITDNHQALLLSLPQSQQRAAEEYVSK
                     MRSERRVIEWLTTRVLLFELLGEEKMIDNRPDGRPFLIDGSYKISISHTKDYAAVLLH
                     KQHSVGIDVETISGRVSKLADKFISENEYIDHSQQVIHQLLHWSAKETMFKMMEESEI
                     DFKEHLHIHPFNPERKGVFQANESKTPQQKTFQIHYEVLPDAVLTWAVDR"
     gene            complement(19977..21899)
                     /locus_tag="PLI30_00195"
     CDS             complement(19977..21899)
                     /locus_tag="PLI30_00195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019538438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HOI78005.1"
                     /translation="MESALFHGILKEYWGYDSFRPLQEEIIQSVWERKDTLGLMPTGG
                     GKSLTFQVPALAMDGLCLVVTPLIALMKDQVDNLREKGIKATAVYSGMSRDEIITALE
                     NCIFGDYKFLYVSPERLSSDIFLTKLRAMNICLLVVDESHCISQWGYDFRPSYLNIAD
                     VRDRLVNVPVLALTATATCEVADDIQEKLHFGKKNVFRTGFERDNLSYVVRTAENKMA
                     ELIHILKSVKGSGIVYVRSRRETKEIAVALQKTKISADFFHAGLSHEEKVYKQNAWKT
                     GECRVIVSTNAFGMGIDKPDVRLVIHMDLPNSLEEYYQEAGRAGRDGERSYAIVLYTK
                     ADSVKLKKRVSDSFPRKEFIIRVYEALGNYFQVAVGSGGSNVYDFNLHEFCHVFKFSH
                     LQTHHALKILELAGYIEYTEEVDSRSRLRFLAFRDELYSLNLSKDNDELVHTILRNYT
                     GVFSDDVYIDEAMLAIRLGRTREEVYQALIHLARLRYIYYVPHKKTPFIVYTSSREDT
                     QFVAIPKSVYEERKKRFEKRIASMADYAENERICRSRMLLIYFDEKNPKDCGSCDVCL
                     RKTETGLTNYEFNKIETLLAESLEATSPQRLDNLLQSIPGFPAEKVIKVIRFLVDRGR
                     LSLNDDEIALSVHRPG"
     gene            complement(21927..>22814)
                     /locus_tag="PLI30_00200"
     CDS             complement(21927..>22814)
                     /locus_tag="PLI30_00200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007657149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHHA1 domain-containing protein"
                     /protein_id="HOI78006.1"
                     /translation="TVNDIAFKIGPRINASGRMMNGKEAVDLMLAGDMSQAREKAVNI
                     DKYNEDRRELDKRITDEAVDFVDNRFNIAEHKSIVLYNETWHKGIIGIVASRLTEKYY
                     RPAIVLTKSGGMISGSARSVNNFDVYKAIEACKDILENFGGHTYAAGLTLKEENLSEF
                     KRRFDEISFEEIESKMMQPQITVDAEISLNAITPRFVQELALFNPFGPENENPVFVTR
                     GVLDAGGSKLVGRGFHHIKLELVDRTVSEPVQAIAFSSDEHFKKIKEKQPVDVCYTIE
                     ENRHGGSTYTQLLVRDIKG"
BASE COUNT         6350 a         5299 c         4873 g         6292 t
ORIGIN      
        1 ctatagcttc aaacgggtca accgcatttc ccaatcgttt cgacatctta ttgccatttt
       61 tatcgaggac caacccgttt gatatcacat ttttaaaagc cacactgcct tttaccatcg
      121 tactgatggc atgcaacgta aaaaaccatc cccgggtctg atctactccc tccgcgataa
      181 aatcggcagg ataaactttt gcaaattcct cttcagttat atgaggatag aagaactggg
      241 caaagggcat ggcaccgcta tcgaaccaga catcaatcag atcgagttca cggtgcatcg
      301 gctttccggt ttcggagacc agcacaatat tgtccacata gggacggtga aggtcgattt
      361 tcccgtattc cagatccctg tactccttca ggtcaaaatc cttccagggc agttcgctca
      421 tcacaccggc atcgagtgct ttttgcattt cgttgcacag ctcttttacc gaaccgatac
      481 atttctcctc ccggccgtct tctgtacgcc agatcggaag tggtgttccc cagtaacggc
      541 tgcggctcag gttccagtcc tgcaggtttt cgagccactt tccaaaacgg cccgttccgg
      601 tggactgggg cttccagttg atggtgtcgt tgagttccat catcttttca cgacaagctg
      661 tcgagcggat aaaccagctg tcgagcggat aatacaacac gggcttatct gttctccagc
      721 agtgtgggta gctgtgaaca aatttttcga tgcggaaagc acggttccga agtttgagag
      781 aaacggacag gtcgatatcc agtgttactt catcagcaga caacgcttga tcgtattcat
      841 tcttcacgta tcttccggca aactccttgt acaaatcaac attcacgttg ttttgtacaa
      901 aaccaccgtc caggtcttcc aggcggaaga atttcccggt caggtcaacc attggccggg
      961 tgtttccgtc tttgtcaaca acggttagtc cggggacatc gttttcttta cccaccttcg
     1021 cgtcatcggc tccaaaagta ggcgcaatgt gtacaatccc cgtaccgtct tcagttgtta
     1081 caaaatcgcc acaaacgact ttaaatgctt tttccgtcac ctttatccag gggaaaagtt
     1141 gttcatattc caggccggcc aattccgtgc ctttccatgt tttttccaac acccggaacg
     1201 gtatattttt atccccttcc tgataatcct ccaattttag ttgagcgttc ttcgacggga
     1261 aataagaatc gagaaggttt ttcgccaaca ccacagtcac cgggctaccc gtatattgat
     1321 tgaacgtctg aacagcaaca tattcgatgc tcggacccac cgccagcaac atattcgacg
     1381 gcagtgtcca gggtgtggtt gtccaggcca gaaaaaaaac ttcgccaaac tgtttccact
     1441 cgggtagcgg atggattact ttaaactgtg ctgtacaggt ggtatcttta acgtcgcggt
     1501 aacaaccggg ctgattcagc tcgtgcgtac tcagcccggt tcccgctgcc ggcgaatacg
     1561 gttgaatggt atagcctttg tagagatatc cttttttgta aatttcttta agcagatacc
     1621 ataacgtctc tatgtaacga ttatcatagg taatgtacgg atcgttcata tccacccaat
     1681 aacccatttt ccgcgtgagg tcttcccatt ctttggtata tttcatcacc tctgtacggc
     1741 aagcattgtt gtactcttct accgaaatgg ttttaccgat atcctccttg gtaataccaa
     1801 gcgttttttc cacacccagt tccacgggaa gtccgtgagt atcccagccg gccttccggt
     1861 ttaccaaaaa acctttcatg gttttgtaac ggcagaaaat atctttaatg gaacgtgcaa
     1921 ttacgtggtg aatacccggc atcccgtttg ctgaaggcgg gccttcataa aacacaaact
     1981 ccggatggcc ttcacgtatc tccagacttt tgcgaaatac gtactgctca tcccattgcc
     2041 gcaacatttc cttattgatc tccgataaat ttaactgact gtattcggtg aatttcttgc
     2101 tcattgtcaa cgtttttata ccacttaaaa aaattgtctg caaaggtagt gattttttac
     2161 agaaattaga aagctgaaaa ttagaaaata gcaaacaacc ccacacagct tgtcgtgcag
     2221 ggttgagagt cgtaaacctg gaaattaaat tcctaaatcg tattattcct tatcccggct
     2281 gtaattaggt gcttcacgcg tgatcatcac accgtgcgga tggctttcag catatccggc
     2341 agaagtgatg cgggtgaatt tggcttcgtg cagtttctcg atagttgaag ctccgcagta
     2401 acccattccg gcacgaagtc cgccgaccat ctgataaata acctcttgca gcgttccctt
     2461 gaaaggcacc cgggcttcaa tcccctcggg aaccaatttt tttatgtcgt cttccacgtc
     2521 ctggaagtag cggtccttcg aacctttctg cattgcagaa agcgagccca tcccccggta
     2581 ggttttgaat ttccttccgt tgaagatgat tgtttctccg ggtgactcct ctgtccccgc
     2641 caacaacgaa cccatcatta cggatgaacc gccggctgcc agcgctttca cgatatcccc
     2701 cgaataacgc aatccgccat cagcgatgag cggcacccct gatcccttca acgcctgtgc
     2761 cacatcgtaa atagccgaaa gttgtggaac gcccacacca gctattaccc gcgtagtaca
     2821 gatcgacccg gggccgatac ctaccttcac tccgtctgcc cccgccttta ccagaaattt
     2881 tgctgcatcg gccgtggcaa catttcctac cacaacgtct atttcaggaa aagttttttt
     2941 gataagtttt agcatatcac caacaccttt cgaatgtccg tgagcggtgt ctataacaat
     3001 ggcatcaaca ccggcatcga taagagcggc agccctttcc acagagtcga aagtcactcc
     3061 gatccccgca gcaacccgca acctaccttg ctcatccttc gatgcataag gcttgtcttt
     3121 ggctttggta atatctttgt aagtgataag ccccaccaac cggtaattgg agtcgaccac
     3181 aggaagtttt tcaattttgt gctgctgaag aatttcggcg gcagcttcca ggtcggttgt
     3241 ctgacgggtc gtaatcaagt tttcccgggt catcacttca cgaataagct tatccatgtt
     3301 gcgttcaaaa cgcaagtcac ggttggtcac tatcccaacc agcgtattgt cctcaataac
     3361 gaccgggatt ccaccgatct tatattcagc catcatttct agtgcatcgg caactgtttt
     3421 ctcaggatgg atactgatgg gattcagaat catgccgttt tccgcacgtt ttaccgcacg
     3481 cacctgtttt gcctgatcgt caacggtcat gtttttatgt atgaccccca tacccccttc
     3541 acgggcaata gctatggcca tagtcgtttc cgtaacggta tccatagctg ccgacaccat
     3601 cgggatgtta agcgggatat ttctggaaaa atgggtggag agatctacgt ttcggggaag
     3661 aacttcggaa taaccgggga ccaacaacaa atcgtcgaaa gtcaaccctt ccattgtaat
     3721 tttatctgca ataaatgaca taatctgtat gtttaaattt tattgcatgc aaaagtaact
     3781 ctttttattg aatgcaacga ttttaaaacg ttaattttat ttcataccgt tgttttttta
     3841 aaacggctaa accactgtta tagaaatatt caatagctga taagcgaaca aaaaaacagg
     3901 gttgacggtt aatttttata tctttggccg gtcattcata attaacaaaa atataattta
     3961 caaatatgtc agtattagta ggaaaaagag cgcctgtttt ttgggcaccc gcagttcttg
     4021 acgggaacat cattgaggat aatttctcat tggacaatta tttaggaaaa aaatatgtgg
     4081 tttttttctt ttatcccgca gattttacat ttgtctgccc aactgaattg cacgcctttc
     4141 aagaaaaaat agcagcgttt gaaaatcgcg acacagtagt tgtggcttgt tctaccgact
     4201 ctgcagtttc acactggaaa tggctgcaaa cgccaaaatc agatgggggg attcagggtg
     4261 tcacctatcc cattgtagct gatcatgcgt tgacaatctc catggcttac gatgtacttg
     4321 caggagaata cgataccgat tcatcggata atccgatttt cgtaggtgcc cctgaagcat
     4381 accggggatt gttcctgatc gacaaagccg gcatagtccg tcatcaggtt gtgaacgata
     4441 tgcctttagg aagaagcgtt gacgaggtac taagggtgat cgacgcactc cagtttaccg
     4501 aggagcacgg tgaagtgtgc cccgccgatt ggcacaaggg ggacaaaggc cttaccgcaa
     4561 ctcaggacgg gattgcttcg tatctttccg aaaagtaact ccacacggag tccggaactt
     4621 tttgaacaag acattgcctt gtccgatcgt tttaaaacag cattgttccc gtttttaagg
     4681 gctctgctgt tttctgcatt tggaaaatat gctgcaatag gctaaaaaaa tgtgtgtttt
     4741 tgagacttta ttgtgttgtt tttgagaaaa tagataataa tatccggttt tttaattact
     4801 tttgcaatcg atttacctca gacgtccggt agcggacttc tggcttcaac cctcaaactt
     4861 ctcacatata tgaatcctct atcagcaaga ctgcaatcac tctctccatc cgaaacattc
     4921 gcgatggcac aaaaaagcaa cgagctaaaa gctcagggaa ttgacgtaat taatatgagt
     4981 gtcggcgaac ccgatttttc cacacccgac cacataaaag aagccgccaa gaaggcgata
     5041 gacaataatt tttccttcta ttcacctgtt ccaggatttc ccgaactaag gaaagccatt
     5101 tgcgcgaaac tgaaaaacga gaacaacctg gatgttgatc cgacacaaat agtggtttcg
     5161 agtggagcca aacaatcgct ctgcaatgtc atcttatcca ttgtagataa aggggaagag
     5221 gtaatcattc ccgcccccta ctgggtgagc tatccggaga tggtaaaaat tgccgaaggg
     5281 aagccggtct tcgtgtatgc cggtatcgaa caggatttca aaatcaccgc agaacaactg
     5341 gagaagtcca ttacccccaa aacaagggca ttgattttat gctctccgtc taacccgaca
     5401 ggaagtgtct acacgcgcga tgaattgaaa tcactggccg ctgttttggc aaaacacccc
     5461 ggtatctatg ttatttccga cgagatctac gaacacatca actacgtagg aagacacgag
     5521 agcatcgctc agttcgaaga agtccgtgag cgggtaatcg tagtcaacgg agtttccaaa
     5581 ggatacgcca tgacaggctg gcgcatcgga tggattgctg cgccaaaatg gattgcttcc
     5641 gcctgtgggt tactgcaggg acaatacaca tcgggagcat cgtcgatctc acaaaaagca
     5701 gccgaggctg cctacaccgg gaatcaggat tgtgttgaag aaatgcgtgt tgcatttgaa
     5761 aggagaaaaa acctgatcac aaagctcctg aaagaaattc cggcattgaa agtcaatgac
     5821 cccaagggcg cgttctacgt ttttgttgaa tgcagcagtt acctggggaa acaatttgac
     5881 gaaaagagaa ttgacacagc aactgatttg gccatgtatc ttctggaaca gggacacgtt
     5941 gcctgcgtgg gcggcgatgc tttcggcgca ccgggccaca tccgcttctc ttatgccaca
     6001 tcggatgaga atatagaaaa agccgtttcc agggtaaagg gtgcactgga aaaactgaga
     6061 taaagcgttt cgcagtttct acacgagagt gcgttatcgc aatttattag ctaaaaacag
     6121 gcggcagcaa tgccgcctgt ttctttagaa cgtgacgttg atcatcaccg gaaaatgatc
     6181 ggacggaaaa cgggcgtatg gcaattcatt cagtacgccg tatttattca ctttcacccc
     6241 ttccgtaacc catatataat caattcgccc cttcatggga gaattcaagt tcaatgcatt
     6301 aaacgttcct tccgtcccgt aaggcggctg caccgaaatc aagtaggaat ctttcagcct
     6361 tccgtcattc ctcaggattt gtatgggttc atcatcgggc gtagcgttaa aatccccggt
     6421 gcagaaaacg gtattatctc cggcaatctc ttctattttc tttaagagca acaacgacga
     6481 atttcttcta gcttcacgcc cctcatgatc gtaatgtgag ttgaaaacaa aaaacacttt
     6541 ccctgtttct ttgtctttaa actttgccca tgaacagatc ctgttgcaac aggtagcatc
     6601 ccagccttta cccggcacgt cagggttttc agcaaaccag aaatctccgt tctccaacag
     6661 atcgaacctc tcttttttat aaacaatggc tgagtgttca ccagcatcct ttccatcgtc
     6721 gcgtcctgaa ccggtgtaag cataatttcc aagctcaaga acatccgtca gctgatgctt
     6781 aaacgcctcc tgtgtaccca caatatcaaa atcgtgaaaa cgaaccagcc ccttcaccat
     6841 ctccttgcga tacttccaag cattcggatg atcttttttg gtgtccatca ggacgttaaa
     6901 cgtagcaata ttcatcgtga ggcttttttt ggttgttgaa catcctatta aaattatgct
     6961 gaaaaacagc agaaaaaaga acggtaatcg tttcatctga atatgtttta attattatga
     7021 tcgaatttta gaaaccgttt tgaatttttt catgtcctgt tttatgccta ctttgggatg
     7081 gatttggatt ttcagttcgc acataatagc gggctatttt gaaaaaatga aaattcaaaa
     7141 cagtttttta atccctcaaa attacattta attactatat ttacgaaaag tatcgtaaaa
     7201 taaataccga atattcaagg cccgaagacc gctaaaaaga atgcttctaa ataagagagc
     7261 cgggaaaatt attccttaat ttattctttt ttgatgttat aaaatgctat ttttgtgctc
     7321 tcaattgaat ttccacattc ctattaaagc aaatttttca tttcttgatc acggtaactg
     7381 aaaatcaata taattactaa ataaaatttt aaacaaaaac tttatgagct ggctaattaa
     7441 atcttctatt ggacggaagc tgattatgag tatttccggt ttgtttctcg tcctgttttt
     7501 gatgtttcac agtttgatga atttcgtagt tatcctttcg gccgatgcat acaacaccat
     7561 tgcatcgttg ctgggagcta actggtacgc acttattgca accggaattc tggcgttggg
     7621 ttttattatc cacattatct atgcgtccat cttaacgctt cagaaccaga aagcgagagg
     7681 atcaaacaaa tacgctgttt cacaaccgca aaaaaatgtc tcgtgggcat cgaaaaacat
     7741 gttcgttctg ggtacaatca tcctgggatt tttggtgctt cacttaatcc atttctggtc
     7801 taaaatgcaa ttggtagagt tgatgcacgg gcacaactat gctgctgcag gctaccacga
     7861 tccgacggat ggtgcttatt ttatccgtga actgttcact caacctcttt acagtattgt
     7921 atatatcgta tggcttgttg cgctatggta ccacctcact cacggcttct ggagtgccat
     7981 gcaaaccctg ggatggaaca accagatctg gctgccccgt ctgaagaaaa tatcctatgt
     8041 ggttgcaacc gttatttgcc tgttgttcat ttcagttccg gtttattatc tgctcggctt
     8101 tggcgcctaa ttttacgtat aaaaatttaa aaacattatg ataaacaaag aagacgaaac
     8161 tgcaaaagta gaaaacaaaa ctactctgac agacaacaaa ataaagttgg attctaaaat
     8221 tccaaaaggt cctttggccg aaaaatggtc atcttacaag gcgaaccaaa aactggtaaa
     8281 ccctgccaac aagcgccgac tcgatataat tgtcgtaggg acgggacttg ccggagcatc
     8341 ggcagcagca tcactgggcg agttgggatt caatgtgttg aatttctgca tccaggattc
     8401 acctcgccgc gcccactcca ttgctgcaca gggagggatc aatgctgcaa aaaactatca
     8461 aaacgatggt gactcagtat accgcttgtt ctacgacacg attaaaggag gtgactaccg
     8521 cgcacgcgaa gccaatgttt accgcctagc cgaagtttcc aacaacatca tcgaccagtg
     8581 cgtggcccag ggtgttcctt ttgcccgcga atacgggggt ttgctcgaca accgctcctt
     8641 cgggggtgcg caggtatccc ggacttttta tgcccgcgga caaaccggcc agcaattgct
     8701 cctgggtgct tacagtgcgt tgagccgtgc cgtaaataag aaacaagtaa aactatatac
     8761 gcgttacgag atggttgacc ttgtgatcat cgacgggcgt gcccgcggta tcatagcccg
     8821 taatctcgtg accggaaagt tggaaagatt tgctgctcac gcggttgtga tcgctaccgg
     8881 cggatacgga aataccttct tcctctccac caatgccatg acctccaacg gatcagctgc
     8941 ctggcagtgc tacaaaaaag gggcttattt tgccaatccc tgcatggcgc agattcaccc
     9001 gacgtgtatt cctgttcacg gtgaattcca atcaaaactg acgcttatgt cggaatcgtt
     9061 gcgcaatgac ggcaggatat gggttcccag aaagcttgag gatgctcagg ctatccgtga
     9121 aggaaaacta aggccgacag aaataaagga agaagatcgc gattactacc tggaacgccg
     9181 ctaccccgct ttcgggaacc ttgtcccccg cgacgtggca tcacgcgcag ccaaggaacg
     9241 ctgcgacgca ggctacggag tcggaacaac cgggttggcc gtttatcttg atttcgccga
     9301 tgccatcaaa cgtctgggaa aagatgtggt ggaagcccgt tacgggaacc ttttccagat
     9361 gtatgaaaag atcgtcgacg acaacccata tgaaaccccg atgatgatct atcccgccat
     9421 ccactatacc atgggtggaa tttgggttga ttacgaactg atgacctcca tacccggact
     9481 ctttgccatt ggcgaagcca acttctccga tcacggcgcg aaccgcctgg gcgcctccgc
     9541 attgatgcag ggattagccg acggatattt tgttctgccc tacaccatac aaagttatct
     9601 gtccgaccag atcagggtac ccaggttcag caccgacagc cccgagtttg aaaaagcaga
     9661 aaaagatatt gatgcacgta tggaaaggct catgagcata aaaggcaacc attccgtgga
     9721 ccacttccat aagaaactcg gacacatcat gtgggattta gtcggaatgg gacgtgaagc
     9781 agatggctta aagaaagcca tcgaagaact gaaggttttg aaaaaggagt tctgggcaga
     9841 tgtacggata ccgggcgaaa agagctcgtt gaacattgag ttggaaaaag cacttcgtct
     9901 tgccgatttt atcgaaatag gcgaacttat ggctcacgat gcgttagaca gagaagaatc
     9961 ctgcggagga catttccgtt tggaacatca gacagaagaa ggggaagcct tacgtcacga
    10021 cgaccggttc tcctatgttt cctgttggga atatcagggc gaagaaaaag ctcccgttat
    10081 gtacaaagaa cctctcgact atgaatttat cgtgagacaa caacgcaatt ataagtcgta
    10141 atttaaaaat cgtatcatcc tatggacaaa aatataaata tcaacgtaaa agtctggcgt
    10201 caaaaaggcc cgaaagccaa aggaaatttt gagacctacg cattgaaaga gatctcgcag
    10261 ggcagttcat tcctcgaaat gatggacatt ctgaacgaac aactcatcaa cgaagggaag
    10321 gatccggttg ttttcgatca cgattgccgc gaagggattt gcggaatgtg cagtttgtat
    10381 atcaacggcc atccgcacgg acccgatgaa gacattacca cctgccagct ccacatgcgc
    10441 aaattcaacg atggagacac cattacggtt gagccttggc gttcagcggg atttcccgta
    10501 atccgggacc tgatggtcga cagaacagct ttcgataaaa ttatccaggc tggtggctat
    10561 gtttcggtaa acaccggtgg tgttcccgac ggaaatgcaa ttccaattcc caaacatgcc
    10621 gcagatgaag ccatggatgc tgccgcctgt atcggatgtg gagcctgtgt ggcaacctgc
    10681 aaaaacggat cagccatgct tttcgtttcg gcaaaagtga gccagttggc tttgctcccg
    10741 caaggacaag tagagcgtgt taaacgcgcc aaggcgatgg tagcaaaaat ggacgaactc
    10801 ggcttcggta actgtaccaa tacgggtgct tgtgaacagg aatgtcccaa gaatatatcc
    10861 atcagccata tcgcccgctt gaaccgcgag tttctgaaag caaaaataaa ggactaatct
    10921 gctctaaatc gatttacaaa gcgttttgtt cttgtgcgga acagaacgct tttcttttgt
    10981 gagctaatcc gtaaatagga ttaattaaca ttctggattt aacttttttc cgttttaact
    11041 gtcttactat atagatttgc atttgcaaac ggtttgatta tttactaaaa cacattagtt
    11101 atgaaaacaa agaaattatg gatgatcctc ctggcctttc tgttgatggg aggtacggta
    11161 agtgctcaat tacggtttgg aataagagga gaagtaggta tcaacaaacc ttcctttacc
    11221 aaggacgtat attcagtaga aaacatgaat gacttcaagg tgggccctac attggaattt
    11281 atgctccctg ttgttgattt tggaattgaa ggatcggttt tatacagcaa cgagaaaatg
    11341 aatataaaaa ccgtaaatga tcagggaatc aaaactgtag ctgaagatat ctccaatcat
    11401 tccatcgacg ttcctgtaaa cttaaaatac aaaatcgggc ttatctcccc ggtaaaaatt
    11461 tttctggctg ccggtcctta cgctaatttc tggctagcgg gggatgattt taaatctgaa
    11521 gtctttatgg acaaggtaga ggcaaaaaaa tttcaagcag gcgttaatct gggagccggc
    11581 ctggaagtga tcaaccacgt agctgtaggc tttaattaca ggatgaaatt aaccgatgac
    11641 tactctgtat atgaacccgc ctttgaagat atttttaatc agaaggatgg attttggtcc
    11701 attaccgcta ccctgttttt ctaaatgaaa catgttgttt gctgatgtaa tcctgccact
    11761 tcccttagcc gaatctttta cctacgttgt tcctgcagag atgcaggaaa agataggtac
    11821 cggattcagg gtggttgttc ctttcgggaa acggaagtat tacacagcgg tggtcctaag
    11881 gctacaccat acgccgccaa aaggagttga aataaaagaa atccattccc tcaccgattc
    11941 acatccggtt gtaaacgaac atcagattcg tttgtgggaa tggatctctt tttattattt
    12001 gtcaccgctg ggggatgttt ataaggcagc cttgccatcg ataatgaagc cacaggatct
    12061 ggaggaaagg tatagatcca gaacggaaac ctatatccgg ctcaacacag gcatccatga
    12121 tattgatcgg cttaccgggc ggtctaaaaa acaagctgaa ctttatcaag cacttaggca
    12181 attatttttg gaagaaggca gggagagcat tccgaaaaag gaaattactg caaaaaccgg
    12241 ctacagcaat actgttttaa aggggttgac ggagaaaaac atagtgatcc agtacccgga
    12301 agaggtgagc cggatcgata cgcattttgt atcatcgcgt aaggcgttta acttaaatga
    12361 ccatcagcaa aaggcactgg aagagataaa ccgttcattt gaagaaaaaa atgtttgtct
    12421 tttacacggg gttacatcga gcgggaaaac cgaaatttac atccacctca tcgaagagca
    12481 gctgaatcaa gggaaacaaa cgctttatct ggttcccgaa atcgccctca ccacacaact
    12541 gacttcacgg cttcaggcgg tttttggaga taaactcgga atctatcact ccaaaataaa
    12601 cgataatgaa cgtactgaga tctggcaaaa aatgctatcg gattcgcctt atgaaatcgt
    12661 tatcggcgta aggtcgtcgc tattcgtgcc tttccgccaa ctggggctgg taatcgtgga
    12721 tgaggagcac gaaaccagtt acaagcaaca ggaaccagcg ccccggtatc acgcgcgtga
    12781 tacagccata gtactggctc aaacttttgg tgcaaagaca ttgctaggat cggctacacc
    12841 gtccatcgaa acgtattaca acgctaaaac cgggaaatac ggacttgtaa ccctcgaaaa
    12901 acgatttgaa aatatcgaac ttccccgcat cacactggag aacacccggg agctccgccg
    12961 aacaaagaaa atgaaaacgc tacttgcccc tggactgatc gaaaagataa acacggccct
    13021 gggatcgggc gagcaggtga ttctgtttcg caaccggcgc gggtttgctc ctatgctgga
    13081 atgcaagctt tgcggctgga tcccaaaatg caaccactgt gacgtatcac ttacctacca
    13141 caagaatcgc ggggagctga aatgtcatta ctgcaacaag acgtataaac cggtcggtga
    13201 atgtcctgtt tgtcacgaaa aaagcataga gcctgtcggc atgggtaccg agaagctgga
    13261 agaagaagtg atacaacttt tccccagtgc cactgtcgca cggatggaca ccgataccac
    13321 acaagggaaa tacgcttacg agaagataat ctccgatttt cagaaaaata aagtgcaaat
    13381 tttagtaggt actcaaatgc tctcgaaagg gttggatttc gagaatgtga gtcttgtagg
    13441 gattgtctca gcagacggat tgctcaatcg acccgatttc cgttcgcacg aacgcggctt
    13501 tcagctgatg ttacaggccg ccggacgtgc cggcagaaaa aaccggcaag gtgaagttgt
    13561 cattcagacc gccgatccgg ggcaaccgat ttaccgatac ctggagacaa acgattacga
    13621 aggtttctac cactcccagc tagaggaacg aaagttattc aactatcccc cttacaagcg
    13681 gttaatttcc attgtattta aacacaaaga cgaacacaaa gtggaatcgg ggtctgcatt
    13741 tttcgcccgg ttattacaac aatcgcttgg tgaaatggtg ctcggtccaa acaaaccgtt
    13801 tatcagccgt attcaacaat atcatctccg ccagattctg ctcaagctcg acaaccattt
    13861 gtcccctgcc aaaacccgac aattcattaa atccgttgaa tcccgctttc gcgaaaaaca
    13921 ggattttaaa tatttggtgt tgtattttga tgtggatgtg atgggttaac gcaaaaaacc
    13981 gaactgcctc cgcaattcgg tttttacttg acgtttcgct gacgcgtaac tttatttcat
    14041 ttcaatttct tctttcaggt ctatttcgtt attttctttg tcgtaaaaca catattgcag
    14101 gaatcccaaa ctctgattgg gtattacttt caaagcaaca ttgtatttct ttttccattt
    14161 gtatttcaac gagaataaac cttgttgaat ataagctgca acataaggat gtatatgcag
    14221 actgaatttc ttcactttta gcttgtttac taaataatcg attttaccct ccaacgtgtc
    14281 ggtaaataaa atcgacgatt taatcttccc ttttccaaag caagtggggc acacctcgct
    14341 tgtggctaat tccatttcgg ggcgcacgcg ctgacgcgtg atttgcatca acccgaattt
    14401 gctcagcggg agaattttgt gtttcgctct gtccgatgcc agaaactcgt tcatcttatt
    14461 gaacagcttg ttgcgatgat cgcccgaact catgtcgata aaatcgacta caatgatgcc
    14521 acccatatca cgcagacgca gctggcgggc aatttcctca gcggcagcaa cattcacctc
    14581 aaaagcactg tcttcctgac cgagcgattg tttattgcgg tttccgctgt tcacgtcaat
    14641 aacgtgtaat gcctcggtgt gctctataat gagataagcg ccgttcttaa acgtaacggt
    14701 tttgccaaag agcgatttaa tctgtttggt gatgccaaag ttgtccaata ttggtgctgc
    14761 tccgttgtaa tgtttgacta catttttccg gtcgggagca attaaaccga catactcggc
    14821 aatctgtttt gccacatcct gatcattgat gtggatctgt tcaaacgatg ggctgaaaat
    14881 atcgcgaagc aatccgaccg tccgtcccgt ttcttcaaaa accagcgacg gtgcttttgc
    14941 ttttggcaat ttctctgcag tatcttccca acgtttaacc aatactttta actctgcatc
    15001 aagttctgcg actcttttct cttcggcgtt agtacgaatg attacgccaa agttcttggg
    15061 cttaatgctt tgcacgagtt gtcgaaggcg tgcgcgttcc tcacgcgatt taattttttg
    15121 cgaaaccgaa acgcgatcga taaacggtat cagcaccatg aatcttccgg caaaagaaat
    15181 ttcggctgtc aacctcgggc ctttggtcga aataggttct ttggcgacct gcaccaggac
    15241 ctcctgtccc acctttaatt tgtccgatat ggaaccgtct ttctcgattt cgggctgcaa
    15301 tttcattttc tgcaacaacg gaatcttttt cttgtcttca atcgttttca gaaaactcga
    15361 aacagagtta aattctaccc ctaaatccaa atagtgaaga aaagcgtcct ttttgtgtcc
    15421 tacatctaca aatgcagcat ttagcgcagg catcaatttt ttgaccctgc ctaaatagat
    15481 gtttcccacc gcgtaagatt cactctggct ctcttgttgt aactctacga gttttttgtt
    15541 atcgagaacc gccagcgtaa tctccttagg ctgaacatcc actacaagtt cgcttgccac
    15601 aatttttttt gtttttaaat gtttgattaa tcaggctttc tgctcctctg agcagaaagc
    15661 agtgttctca cgaacacttt tgaaatgttt atacacaaag aacaaactct tttgatataa
    15721 tcttttaagt ttgttcccgt atataaagtg tgtcaaaaaa ctttcaaaaa gactatttct
    15781 tctttttatg cctgtttttt ctcagtcttt ttttccgttt atgaacagcc attttatgcc
    15841 tttttctttt ttttccgctt ggcatcttct ttagttttta aaatttacgt aatcctgtta
    15901 tttatttgtg ttatccttta cttgagcaac aaacgttttt gccggtttaa aagcaggtat
    15961 attgtgtgca gggataatga tggtcgtatt tttagaaata ttgcgagcag ttttttcggc
    16021 tctttttttc acgataaagc taccgaaacc tctcagataa acgttctctt tatttgctaa
    16081 cgaatccttt acaacctcca taaaagattc tactgttgcc aaaacagatg ccttatccac
    16141 acccgtattc ttcgcaattt cgtttacaat gtctgcctta gtcattttat ttttttttaa
    16201 tgttagtgat aatatagtta attcttaaaa ttttggactg caaatatata gctttttttt
    16261 catcccaaaa aataaatacg atttaatttt atttttttca cgcattttct tgaatgcacg
    16321 tgtttttaac gtagttttgc accatattct ataagcacaa tgcgtaaaaa agagagcgac
    16381 aagctgatca gcaatatttt acaagagtgg tacgaatgtc atcgacgaat tctgccctgg
    16441 agagattcaa aagacccgta cgtcgtgtgg gtttcagaag ttattttgca acaaacccga
    16501 gtaagtcagg gatatgacta cttcctgcgg tttatggaaa aattcccgga cataaaatcg
    16561 ctagcccggg cgcaggaaga tgacgtattg aaagcgtggc agggattggg gtactactca
    16621 cgggcaagaa acctgcacgc agcagcccgg caggttatgg aaaagtttga cggagttttt
    16681 ccctcatcac acaacgacat ccttttgctc aagggtgttg gagaatatac ggccgcagct
    16741 gttgcatcca tcgtttttgg gctaccctac cccgttgttg acggaaatgt ttaccgggtg
    16801 ttatcgcggc tgtttgccat tgaagaacct atagattcca caagcgggaa gaaactcttt
    16861 accgaaacgg ctacatccat tctagaccaa caagatccgg gtaatcacaa tcaggccatt
    16921 atggaactgg gcgcactgat ttgcaccccc aggcttccca aatgtcagga ctgtccttta
    16981 cagagtgttt gcctggcttt cgaaagaaaa aacatgtccg attttcccgt caaaaaagga
    17041 aaaacaatcc ccaaaatacg ctattttaat tattttcata tcgaacaaaa cggatatacg
    17101 tatattcaga aaagggagca ttcagatatc tggaaaaatc tatacgagtt cccgcttatc
    17161 gagacagaga aacaaagcga tttgtttatg ctacaggacc accctggatt atctactctt
    17221 ttccaagctg ctgacgaaat ttttcttcaa cacaaactcc ggctcaggca cgtgttgtcg
    17281 catcagatta tccttgctga tttttatcgg gttgaattca gcaaacaaga aatgagcggt
    17341 ttggagaaga aatttatcaa aataccagct acatccatat ccgaatatcc tgtatcgcgg
    17401 cttatccata agtatctcga aacaatttac cggtaaaact tgctgaatcc aaaataatca
    17461 ctaattttgt tttgaattga tttacttaaa ccaaattttt tttaagctat gtcggtaaat
    17521 aaagttattt tagtaggaaa tgtagggaaa gacccggaga tcaagtattt tgacaatgac
    17581 aacgttaaag cgaatttccc tttggcaacc agcgagagag gatacacggc agcaaacggg
    17641 acgcaggttc ccgaacgtac ggaatggcac aatattgttt gttggagagg attggcacag
    17701 attgtagaaa aatttgtgaa aaaaggcaca caattgtata tcgagggaaa gatccgttca
    17761 cgttcgtatg acgatcaaaa cggtgtaaaa agatatatta ccgaaatcta tgccgataac
    17821 gtagaaatgc ttaaccgcag agccggagcg ggagaaggtg caggttttca gaacgataaa
    17881 cacgacactt cttacaacga tccggtagat ttgaacgata attctgaagc agatgatttg
    17941 ccgttttaaa aaaacatcgt aattttgcag agaatgtttt aatccctttt tccggattgg
    18001 aacattctct tttgtttcac taaattttcc cccattggat cctgatcctc tttcgcttat
    18061 atatcctgaa gtacaagcag ccgcgctgtc ggcggctgat tatttgttga ttgtacttac
    18121 cctgacacta acattcatca attcgattat ttccggtgct gaaattgcat tcttctccct
    18181 caatcaggaa gagaccgaag gaattaatac cgatgcagat ccggcatccg tacgtgtttc
    18241 tcacttgctc cagaacccgg aaagactgct gagcgctatt gttatttcat acaatgccct
    18301 aaacataacg atcgtcacgt tgcttttcta cctgctgaac cttatcccct acttttcggg
    18361 cagcaacaat aaaattattc tcgagttcct gtttatcatc gctttcatac tcctttttgt
    18421 ggaaattatt ccaaaaacat atgcttctca acgatcgctg gctttcgccc gtagccattc
    18481 ccggtttatt caaatcctga ataaattgat gaatcctttt tcttcgttcc tggtaagaac
    18541 aagccatatc tttagcaaaa gcgttattcg gaaaagatac gaaatatcaa tggacgatct
    18601 atccaaggca cttgaaatta catcgggtga aatcacgcag gagcaggaaa aagatatgct
    18661 cgagggcatt atccgtttca aggacaaaac ggtggaggat atcctggtat cacgaagcga
    18721 tatggtggct atcgatatca acacctcatt tagggatgtc atccatttta ttgtagaagc
    18781 cgggttttcg cgaattcccg ttttcgaaga gaaccctgat aatatcaggg gcattttata
    18841 tgttaaggat ctgttgccac atcttattaa gacagaaaat ttccgctggc aatccctaat
    18901 ccgtccagcc tattttgtgc ccggcacaaa acggattgac gatctgctgg aagagtttcg
    18961 tgccaacaaa aaccacatgg cgattgtggt ggacgagtat ggaggcacca ccgggattgt
    19021 caccatggaa gacattctgg aagagatcgt tggagacatc agtgatgagt acgacgaaga
    19081 agcaaaacct ttctatacca ttgcccagga cggttcttat atttttgaag ggaagacctc
    19141 ccttgaagat ttcatcgaag ttaccggtgt tccggaaaaa gatttcgaaa aactgctgga
    19201 agatgccgac accattgcag gattgatgtt ggagctaaaa ggggatttcc ctaaacgtaa
    19261 ggagctggtg gaatacaaga aatacggatt tcaggctgaa gagatgagta aacgccggat
    19321 aatgaaagtc cggtttattc ctccgaaagg agacagcgac taaaacaatg ttgcttcgca
    19381 aggaacatat cgacggggac ggtcttatcg gcataggaaa aataaccgac aatcaccagg
    19441 ctttacttct ttcgttgcct caatcgcagc agagagctgc ggaggagtat gtttcaaaaa
    19501 tgcgttcgga aaggagagtt attgaatggc ttaccacccg ggttttattg tttgagcttt
    19561 tgggtgaaga aaaaatgatc gacaaccgtc ctgacgggag gccattctta atcgatggat
    19621 cctacaagat aagcatttcc cacacgaaag actatgcggc tgttttgctg cataagcaac
    19681 attccgttgg gattgatgtg gaaaccattt cgggacgggt gagcaaactg gcggataaat
    19741 ttatttcaga aaacgagtat atcgatcact cgcaacaggt aatccatcaa ctcctgcact
    19801 ggtcggcgaa agaaaccatg tttaagatga tggaggagag tgaaatagac ttcaaggaac
    19861 accttcatat tcaccctttt aatcccgaga gaaagggcgt ttttcaagcc aacgaatcca
    19921 aaacacccca gcaaaaaacg tttcaaattc actacgaggt actgccggat gcggtattaa
    19981 cctgggcggt ggacagataa agcgatttca tcgtcattca aagacaatct acccctatcc
    20041 actaaaaaac ggattacctt tatcactttc tcggcaggaa aacccggaat tgactgcagc
    20101 aagttgtcta accgctgcgg agaagttgct tctaaagact cggcaagcag tgtttcaatc
    20161 ttgttgaact cataattggt caatccggtt tccgtttttc gcaaacagac atcgcagctg
    20221 ccacaatctt tcggattttt ttcgtcaaaa taaatcagca acatcctgct gcggcaaatc
    20281 cgttcgtttt cagcataatc agccattgaa gcaatccttt tttcgaaccg cttcttacgt
    20341 tcttcgtaaa ctgatttggg aatggcaaca aactgcgtat cctcacgcga agaggtataa
    20401 acaataaaag gtgttttctt gtgcggcacg taataaatgt agcggagtct ggccaggtga
    20461 ataagtgcct gataaacttc ttcacgcgtt cttcccaacc ggatggccag catagcttcg
    20521 tcgatatata catcatccga aaaaacaccg gtatagtttc tgagaatggt atgaaccagt
    20581 tcatcattgt ctttcgataa gttcaacgaa taaagctcat cccggaaagc caaaaacctc
    20641 aagcgggaac gtgaatccac ctcttcggta tactcaatgt acccggccag ctcaagaatt
    20701 tttaacgcgt gatgcgtctg gaggtgggag aatttaaaaa cgtggcaaaa ttcatgtaag
    20761 ttaaaatcat aaacgttact gccccccgaa ccgacggcaa cctggaagta attgcccagc
    20821 gcttcgtaaa cccttatgat gaattccttc cgggggaagg agtcggaaac ccgtttctta
    20881 agcttgacac tatccgcttt cgtatataaa acaatggcgt atgaacgttc accgtcgcgt
    20941 ccggctcgtc cggcctcctg ataatactct tccagtgaat tgggcaagtc catgtggata
    21001 accaacctca catcgggctt atcgataccc attccaaagg cattggttga aacaataacg
    21061 cggcattcac ccgttttcca ggcattttgc ttatacactt tttcttcatg cgataagccc
    21121 gcatgaaaaa aatcggccga aatttttgtc ttttgtaaag ccaccgcaat ctcttttgtt
    21181 tcccgccggc tgcgcacata aacaatgcca gaacccttta ccgattttaa gatatgtata
    21241 agttcggcca ttttgttttc ggccgtgcga accacatacg aaaggttatc ccgctcaaag
    21301 cctgtccgga atacattctt tttcccgaaa tgcaactttt cctggatatc gtcggctact
    21361 tcgcatgtag ccgttgccgt aagcgccagt acgggcacat tcaccaggcg atcgcggaca
    21421 tctgcaatat tcaggtacga agggcgaaaa tcataccccc attgcgagat acaatgggac
    21481 tcatccacca caagcaggca aatgttcatg gcacggagtt ttgtcagaaa aatatcggaa
    21541 gagagccttt cgggggagac atacaagaat ttataatcgc cgaagatgca attctcaaga
    21601 gcggtaataa tctcatcgcg agacattccc gaataaacgg ccgtggcttt aatgcctttc
    21661 tcacgtaggt tgtctacctg atccttcatt aacgcaatta gcggcgttac aaccaaacac
    21721 aatccgtcca tggccagcgc gggaacctgg aaggtgagtg actttccacc tcctgtaggc
    21781 atcagcccga gcgtatcttt tcgttcccaa accgactgaa taatctcttc ctgaagcggg
    21841 cgaaacgaat cgtaacccca gtattctttc aggataccgt gaaacaatgc cgactccatt
    21901 attcaattgt tattcaaact ccaaacctat cccttaatat cccttaccaa taactgagta
    21961 tacgtacttc caccgtgacg gttctcttcg atggtataac aaacatccac gggctgcttt
    22021 tccttgattt tcttgaaatg ttcatcggaa ctgaatgcga tggcttgtac gggttcggaa
    22081 acggttctat ccacaagctc aagcttgata tgatgaaatc cccgacccac cagcttgctg
    22141 cctccggcat ccaaaactcc ccgggtaaca aagacagggt tctcgttttc cggcccaaaa
    22201 gggttgaaca acgctaattc ctgaacaaat ctgggcgtaa tggcattcag ggagatttcc
    22261 gcatccaccg ttatttgagg ttgcatcatt ttactttcga tttcttcaaa ggaaatctcg
    22321 tcaaacctgc gcttgaactc cgataggttc tcttccttga gggtcagacc ggcggcgtat
    22381 gtgtgccctc cgaaattttc caggatatct ttacaggcct cgatggcttt gtaaacgtca
    22441 aaattgttga cagaccgcgc cgatccggat atcattccac ccgatttggt aagaacgatt
    22501 gccggacgat aatatttctc cgtcaacctc gaagcaacaa tgccaatgat gcctttgtgc
    22561 catgtttcgt tgtacaaaac gatgctctta tgttcagcaa tattaaaacg gttatccaca
    22621 aaatcaacgg cctcgtcggt aatccgtttg tccaactcgc ggcgatcttc gttgtattta
    22681 tcgatgttga ctgctttctc tctggcctgc gacatatcac cggccagcat cagatcaaca
    22741 gcctccttgc cgttcatcat cctccccgaa gcattgatcc tcggaccgat tttaaaggca
    22801 atgtcattaa ctgt
//
LOCUS       DAOSDR010000003        22508 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrimonas sp. isolate AMR_MDS_1826
            NODE_1009_length_22508_cov_4.344854, whole genome shotgun sequence.
ACCESSION   DAOSDR010000003 DAOSDR010000000
VERSION     DAOSDR010000003.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863355
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrimonas sp. (wastewater metagenome)
  ORGANISM  Petrimonas sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Dysgonomonadaceae; Petrimonas.
REFERENCE   1  (bases 1 to 22508)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 22508)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:10:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,720
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 35
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22508
                     /organism="Petrimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1009_length_22508_cov_4.344854"
                     /isolate="AMR_MDS_1826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2023866"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1146)
                     /locus_tag="PLI30_00205"
     CDS             complement(<1..1146)
                     /locus_tag="PLI30_00205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019539456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOI78007.1"
                     /translation="MKKTIFVLFFALATHLAMAQRTVYHHQPGKLFNQGKEMFLEGNY
                     TGAQDLLSQFITESTDRLLIEEAKYMMAVSSFYKGVKNSGERMKEYLDEYPESIYHHQ
                     VKFLIGSYHFDEKDWKLARFWFDQADLDYLKPSEQEDFSFRSAYANLQLDNKDEAYRL
                     FGLLSQNSKKYRNAGNFYAGYIDYTNGNYDAALRRFEGLRNHPEFGEQVAFYSAQSTF
                     FNNKLDEAIRLADSFLKAYPRSEHNTEMYRVLGNSHYRQGRATTAIPYYENYFGSTAR
                     PLRGDAYFMGLSYMEARRFNEAVQMFQKAVGERDALTQNAQLQIGQAQLKLGQKQQAQ
                     MAFEAASRDNFDPQVRETAMFNYALLAHETNFSVFSESIALFENFLKE"
     gene            complement(1184..1717)
                     /locus_tag="PLI30_00210"
     CDS             complement(1184..1717)
                     /locus_tag="PLI30_00210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019539457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HOI78008.1"
                     /translation="MKHPLHQFHFCPECGSGNFSENNLKSKKCTDCGFTYYFNSSAAV
                     VAIIENEKGEVLIARRAKDPAKGTYDLPGGFVDMYETAEEAIAREVREETGLLITSSK
                     YLFSIPNIYVYSGFEVHTMDLFFQCKSLDDSVMTPMDDVSDLLFIDKRELRPADFGLI
                     SIRKGIEKILEDVRIRK"
     gene            complement(1714..2823)
                     /locus_tag="PLI30_00215"
     CDS             complement(1714..2823)
                     /locus_tag="PLI30_00215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008772695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mrp/NBP35 family ATP-binding protein"
                     /protein_id="HOI78009.1"
                     /translation="MAIYPQLIIDALKNVRYPGTGQDIVSAGMVEDDIRIDGMKVSFS
                     LITERQNDPFVKSLVKMAEQAILTYADPKIQIKGNISVKSKQAPRPALPDLLPDVKNI
                     VAVASGKGGVGKSTVCTNLAIALAQKGYKVGLLDADIFGPSVPRMLGVEDVPVYTEKM
                     EGRDLIVPVENYGVKMLSIGFFVKKEDAVVWRGAMASNALKQLITDADWGELDYFLID
                     FPPGTSDIHLTLVQTVPITGAVIVSTPQEVALADARKGISMFTGEKVNVPILGLVENM
                     AWFTPAELPENKYYIFGKDGCKQLAEQNGYALLGQIPIVQSIREGGDDGQPVALQSDS
                     ITGMAFARLADNVVEAVEKRNRELPKTGIVEISRK"
     gene            complement(2842..3582)
                     /gene="trmB"
                     /locus_tag="PLI30_00220"
     CDS             complement(2842..3582)
                     /gene="trmB"
                     /locus_tag="PLI30_00220"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019539459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine-N7-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HOI78010.1"
                     /translation="MGKNKLSKFADMATYPNVFQYTFSTLQNGGFPLKGKWKTDYFRN
                     DNPVVLELGCGKGEYTVGLAQFYPEKNFIGIDIKGARMWSGARQALSENLTNVAFLRT
                     HIELIRHFFAENEVSEIWITFPDPQMSKINKRLTSTRFMNTYSQILADKGVIHLKTDS
                     NFLYIYTKAMIEENGLAALADTDDLYRSGTNDKVLNIQTFYEQQWRSRGLSIKYIRFL
                     CPRKDNWTEPDIEIEKDEYRSFGRDAQL"
     gene            3914..5251
                     /gene="gdhA"
                     /locus_tag="PLI30_00225"
     CDS             3914..5251
                     /gene="gdhA"
                     /locus_tag="PLI30_00225"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009017035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016639 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HOI78011.1"
                     /translation="MKVNDIMTQLEAKHPGEAEYLQAVKEVLTSIEDVYNQHPEFEKA
                     GIVERIVEPDRIFTFRVPWVDDNGKVHVNIGYRVQFNGAIGPYKGGIRFHPSVTLSTL
                     KFLGFEQTFKNALTTLPMGGGKGGSDFSPKGRSEAEIMRFCQAFVTELWRDLGPDTDV
                     PAGDIGVGGREVGYMYGMYKKLAREHTGTFTGKGRSFGGSRLRPEATGFGALYFVNQM
                     CETHKIDLKGKTVAVSGFGNVAWGATMKATELGAKVVAISGPDGYIYDEDGINTPEKF
                     NYMLELRNSGNDVVAPYAEEYPNARFFPGKKPWECKVDIALPCAIQNELNREDAKRLK
                     QNGVTLVAEVSNMGCTAEAVDFFIENKILFGPGKAVNAGGVACSGLEMTQNAMHISWT
                     NEEVDKWLHQIMSEIHDQCVAHGKEDNYINYVKGANIAGFMKVADAMMAQGVL"
     gene            5386..6633
                     /locus_tag="PLI30_00230"
     CDS             5386..6633
                     /locus_tag="PLI30_00230"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OFA family MFS transporter"
                     /protein_id="HOI78012.1"
                     /translation="MSKKWLIAVTGTFTHLLLGTVYAWSYFQNPISESAGWSNAQTAW
                     AFSLSIFMLGVTSAWAGNKMSVYGPRRLSMIGGALYAFGYLVSGFALAHRYLFLLYFG
                     FGIMGGIGLGLAYVAPVATVSAWFTHKQGLATGMVVMGFGFGALVMSKFLAPLLLQLS
                     DGNLSTTFYYVGAVMIVLIPVLASFLQLPKPDKTHEKKPERISIARHIQSKPFIVVWL
                     IFAINIVAGMIFISFQSPLLQDILKMRMSDGTNFSDGETMASLAAAGATLIAVSSVFN
                     GMGRFAWGSISDKIGRMYTFRILLALQAVIFGLLILVHHPVVFSVLVCIVLLCYGGGF
                     GVLPSLTKDMYGSKLMPSLYGAFLTAWSMGGIIGPQIVAFMKDNYAEKAGLFAFVVGG
                     GLLIVGFFTSFLYRRKDVINLEN"
     gene            6639..9506
                     /locus_tag="PLI30_00235"
     CDS             6639..9506
                     /locus_tag="PLI30_00235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019539482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HOI78013.1"
                     /translation="MTPFLLRIAQAFYSKYENEIYKLSFVFPNKRAGVFFQKYLAEIA
                     GKPVFSPRIITIQELFESLSAYRTADKTEMLVMLYEQYIRIGRSEELFDDFLYWGEIL
                     LNDFNDVDKHLADARQLFRNIHNLKSMDDDLSYLTPEQINAIRRFWTAFMPYEGNETK
                     QEFMETWQILFELYTAFRETLHKKGYAYEGMLFREVAERAKEKEDIELPFSELVFVGL
                     NALTPTEVELMKYLRNRGVADFYWDYESPLVRDKKNRSSRWIQENLSRFPSKLEFGSG
                     IPNAEKPDINLIGIPSGVGQAKTVNRLLSQLLNNKSITGSNAGLNTAIVLPDENLLLP
                     VLYSIPQEIEKINVTMGYSLQHSSVASLTESIARLHHNTRTMDGETAFYFRFVLSVLN
                     HPLVTRIAPSETEELKAYIQQHNRVILTVSELGTHPFLKLIFSPIMDWTKIGEYLKSI
                     LSEIYRHLTSEKHPDEGEFTGDTRSIDLEREFIVQYYKSITRLQDTLSSVQNMSIDTY
                     FRLFKRLTENLSVSFSGEPLSGLQVMGVLETRVIDFENLIILSMNEGVFPLKNATNSF
                     VPYTLRKAFDLPTYEHQDSTYAYHFYRMISRAKRIFLLYDTRTEEMQTGEVSRYFYQL
                     KYLYSQHFHLKESVVSYNVSAPHVSPVSVEKTPEVMRKLQKFRAGGDASLSASLINNY
                     INCPLQFYFSAVERLSEEKEVQESVESDVFGTMYHAIMQHLYNRYRGKSVLPDTLNAI
                     IQNDDYLTDLIERSFAEHYFKQKDKPRALTGHHFLIGEILRDYVKQTLRFDTSFTPFE
                     YVDSEFVFRSIHQVSGDLSVNIKGSIDRIDKVAGRLRIIDYKSGKGELNFRNIEQLFD
                     NTKVNRPYQILQVFIYALFYNSPLPVSPAVYYLRNIFREHNPAITFAGEPITDISVYL
                     SAFTEKLNVFLEEIFDKDIPFVQTQNEKNCEWCAFKDICRR"
     gene            9641..10057
                     /locus_tag="PLI30_00240"
     CDS             9641..10057
                     /locus_tag="PLI30_00240"
                     /inference="COORDINATES: protein motif:HMM:NF019911.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEGA domain-containing protein"
                     /protein_id="HOI78014.1"
                     /translation="MLAGAILFASCSSTTLIQSSPSGAKVYLNGEYAGVTPYTHTDTK
                     IVGSSTQVRLEKEGYETLNTAFSRNEDADVGAIIGGIFFLFPFLWTMKYKPVRTYELV
                     PSGNASLTGSDNITTAQKGLVKDANGNEYISGVLKD"
     gene            complement(10092..12215)
                     /gene="ovoA"
                     /locus_tag="PLI30_00245"
     CDS             complement(10092..12215)
                     /gene="ovoA"
                     /locus_tag="PLI30_00245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019540394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-histidylcysteine sulfoxide synthase"
                     /protein_id="HOI78015.1"
                     /translation="MKELLTKTIDLRSGMPEEKRREIREYFLKTWAVDELLYTQLKSD
                     EVFYHRGDPLRHIILFYLGHTAVFFINKLFLGKIIDRRINPKFESIFAIGVDEMSWDD
                     LDNNHYDWPPVSEVRHYRDKAKEAILRIIDTAPLLLPIDWESPFWIIMMGIEHERIHL
                     ETSSVLIRQLPLDKVVSDLFGKRCPETGSAPQNELVDVEGATMKLGKPKDHPFYGWDN
                     EYGDYTEEVAGFKAARYLTSNREFLEFMEDGGYEAEKYWTEEGWSWRNFKQAEMPLFW
                     RKDENGYRLRLVAEEVLMPWNWPVEVNYLEAKAFCNWKSEKTGQAYRLPTEAEWYRLH
                     QVSGLKDVMGWETTPGNINLEYGTSPCPVDKFQQGDFYDVLGNVWQWTETPITGFPGF
                     KVHPLYDDFSTPTFDGKHNLIKGGSWISTGNEATLHSRYAFRRHFYQHAGFRYVQSDA
                     PLNIQSADYETDEEVTLSCENNWGDAYSQETNFSKRLAEIVVDVAKGKTESRVLDLNA
                     DTGRLAFELAQYFNDVTALDFTARMIRIPIQLQEQGYMRYTMKDEGELVHFRDVVLSD
                     FGLEKGKGSLLFMQADAMNLKPNYTGYDIIVMPNLLEELSDPGLFLSHIHERVNDGGY
                     LVIVSSYNWDLLRTQREKWLGGFKKDGEPVTTLDGLKAVLGNHFGMEREPFDMELTVR
                     KTSRITENRVLETTVWKRRKRKDNR"
     gene            complement(12287..12856)
                     /locus_tag="PLI30_00250"
     CDS             complement(12287..12856)
                     /locus_tag="PLI30_00250"
                     /inference="COORDINATES: protein motif:HMM:NF024917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOI78016.1"
                     /translation="MKEEALQIRVFGLDLFNYPKFREKILGLYLNAFTTGEHAQYIPH
                     ESAESTLDEMLRNGWGNMAFAEGKLVGVLIAFPLSFDREFPRDRCPQVPVETSVYITE
                     VMTHSDFRGKGVASELFESFLQRVKDNYSDVVIRVWEENKPALSLYEKLGFQPVVDII
                     QTKYRSQNEKFEMRKIYMTKEIRRQELRS"
     gene            complement(12908..14854)
                     /locus_tag="PLI30_00255"
     CDS             complement(12908..14854)
                     /locus_tag="PLI30_00255"
                     /inference="COORDINATES: protein motif:HMM:NF021489.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2207 domain-containing protein"
                     /protein_id="HOI78017.1"
                     /translation="MQRVLCFLFTALFFLSSFAQEEKVYTFRSEVRVDTSGYISVRED
                     VRVYVKGVIFKRGITRALPLIRSDKDGDKVPVDYDIESVKRDGEPESFFTEREGSDRV
                     IYVGNKNVLLDNGWHVYTLDYKTAGQIGFFEGYDELTWNVNALSDYPLDTVSAVIRLP
                     EGAKVLSYRCYTGRYGSSESNCRADTLPDGLLYLEAVSLEPQEMLTVSVGFTKGIVRQ
                     PERRKSGYSKPVPDTFFDKNGLPIVSALIFLLLFAYYFFTWRKYGIDPPKPVVIPQFS
                     PPEGLSPASVGMLYKEKYWDDLITPSIVNLAVKGFLRIKETERSSFLRRKDRVYTLIR
                     TKKDDNMLPREESTVLQNLFKDEDEVVLDGEYDSGIANLMNSFRDDLNKQYKPVLKEG
                     ANRKFMVLPWIMLILYFSSLLYFVRFEPADQIGTFIAVALVSFPVVTLLSLILRRILK
                     KSRIRWFGLVLGAALVVGSLSLLLFFSGNSLSVNAVGFIIGLPLIITSFIAYGFLIKR
                     PGERKLYLQSQVEGLKMYMDTAEEKQLQFFNPPTVTPEIFEQLLPYAIALDMEDVWGE
                     KFESTILSSMQQPQPYQPPWYTGTVLQPARFGHMLNSTLSNTVNHAATPPQTSGSGGG
                     NWSSGSFGGGFSGMGGGGGRVGGW"
     gene            complement(14855..15421)
                     /locus_tag="PLI30_00260"
     CDS             complement(14855..15421)
                     /locus_tag="PLI30_00260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007134224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HOI78018.1"
                     /translation="MGTGIVVLIVLLALAVIFLFYGISIYNRLVKLRTLVEEAWSGIN
                     VQLKKRYDLIPNLVETVKGYATHESETFNKVTQARAHAMSAKDLKSQETAENDLNRAL
                     MNLLAVAEQYPDLKANQNFMQLQHQLSLIESDIEKSRRFYNGSVRDKNIVIDTFPSNI
                     IAGMFNFTKSLFFELENEQEKTAPQVKF"
     gene            15822..16964
                     /locus_tag="PLI30_00265"
     CDS             15822..16964
                     /locus_tag="PLI30_00265"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015547901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018455 - alcohol dehydrogenase [NAD(P)+]
                     activity [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HOI78019.1"
                     /translation="MKNFIFRNPTKLVFGKGQIARLNELIPAEVNLMITYGGGSVTRN
                     GIYDQVKAALKGRKFIEFWGIEANPHVETLRKAIEVGKSENINYLLAVGGGSVLDGTK
                     LIAAGIASDEDAWDIVLKGVAEKQIPFASVLTVPATGSEMNGGAVITRAETREKFAFG
                     GDYPQFSILDPEVTYSLSKHQVACGLADAYTHVLEQYLTTPGQSRLMDRWAEGVLLSI
                     MEIAPKIKENPCDYDLMADYMLAATLALNDFIRMGISQDWATHQIGHELTALHGTTHG
                     HSLAIVMPGTLRVLKEQKHEKLLQYGERVFGITGGSEPERVDKAIEKTEEFYRSLGLT
                     TRLSEENIGNETIETIAKRFNDRGVAFGENQNVTGDVAREILLACS"
     gene            17160..20405
                     /locus_tag="PLI30_00270"
     CDS             17160..20405
                     /locus_tag="PLI30_00270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019540389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HOI78020.1"
                     /translation="MKKLLLFLSVSLLIFRASAVDDARMMRYPDINGNLVAFVYAGDI
                     WTVQATGGEARRLTSHAGTELFPKISPDGKWIAFSAEYSGNRQIWVMPAGGGTARQLT
                     FYNSEGVMPPRGGFDNVVLDWTPDSKRVLFRANRTSFGERNGKYFTISINGGLEEPLP
                     IVNGGFATLSPDGLQLCFTPVDREFRTWKRYKGGRATELWTYDLTNHTSQQITHWAGS
                     DQWPTWHGEYIFYASDQDTRLNIWRYNTQNKENVQITHHKDFDVMWPSGRNGKLVYEN
                     GGYLYVLNLASGTSDKISVSINYDNPNLLPYFKNVKDFVGSFSLSPSGKRALFDARGD
                     IFSVPVENGEIENLTRTQGIREIFPVWSPDGNNIVYYSDATGEYEMYLLENKKGAQPR
                     QLTKGSKAWKYTAEWSPNNKQLVYSDRTLKLWLVDAVSGKQTVIDEASAEEIRDYSFS
                     PDGDWVAYSKSSPNYQSALWLYQISTSKKHQITDASFSDGQPTFSRDGKFLFFTSNRD
                     FNLAFSSFEFDYLYNNAGRIYAIPLRNDGTTLIQYKNDTEPVGGEKTDSVQSGNKNKP
                     PLNVQIDLENIRNRIVALPVQPGNYRIIGAVEEGLLYTSGNKIMRYRINEEKTEEILD
                     GAGNGTLSANGKSFIYRSGSDYGVAKNQAGQKAGANKIDLTNLTMKIEPRKEWDQIYT
                     DAFRIFRDYFYVSNLHGVDWERIQKEYGALLPHVPSRFDLDYILNEIVSETNTGHAYV
                     DWGDIAKVDRINGGLLGAELEADLSAKRYVIKKIYAGENWNESRRSPLTENGIDVKAG
                     DYLISINGKNLTTDDNPYELLENWGNRHVELTVNSSPSASGAKTHTVKTITSEHELRY
                     LDWVNERRALVNKLSGGKIGYIHVPNTAVEGNRELFRGMYSYNDKDALIIDDRYNGGG
                     FIPDRMIDLLNRRTLVYWHRNGLPQPMKTPGIAHDGPKVMLINGYSSSGGDAFPYFFK
                     KTGEGKLIGTRTWGGLVGISGNAGLVDGGYISVPIFGIYDNSGEWIIEGIGVYPDIEV
                     VDRPEALAKGNDPGIEKAVEVLLNELKENPRKPVSVPAPPDRSKWIETEIK"
     gene            20458..20916
                     /locus_tag="PLI30_00275"
     CDS             20458..20916
                     /locus_tag="PLI30_00275"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase"
                     /protein_id="HOI78021.1"
                     /translation="MAKVSLTCMCMIQDLKTNKVLVQDRVLDWKGISFPAGGVEEGES
                     LVEAAIREMKEETGLTVSNLRPCGIVHWYNDKTGDRHLVFNYKTSDFFGELLPETEEG
                     KVFWVTPEELPVLNLSDGFIDRLPMFFEEKNTERFQVWNESGYNPDIKWF"
     gene            21021..22010
                     /locus_tag="PLI30_00280"
     CDS             21021..22010
                     /locus_tag="PLI30_00280"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HOI78022.1"
                     /translation="MDKREFIRKSIAGAVAIGTSGVVSTTFADTTAKPKKTGTIKRKL
                     GKTGFEVYPVVYGGIVSMQDGQKASDNYVAWALDRGINYFDVAPSYGDAEEKLGNSLK
                     PYRKDNFLACKTTQRLREGAEKEFEKSLQLLQTDYFDVYQLHSISTPKDTEQIFGPDG
                     IMEMVEKEKQRGRIRKVGFSAHSQEQAIRAMSMYDFDTIMFPTNWQMIKLDGWGAGAV
                     NEAKRRGMGVLAIKGLIHRRWMEDEKQDSRYQKSWCKPIDVENREFGIAALKFTFQAG
                     ADVIIPPGDFRNFSFCVDNIEEILDSPLTKKEKTLLDNEFLAVKDYPFFDPRT"
     gene            22260..>22508
                     /locus_tag="PLI30_00285"
     CDS             22260..>22508
                     /locus_tag="PLI30_00285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019540388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI78023.1"
                     /translation="MKHFKFAAIISLAWFFASASCVEQKDSDGGDIDQTPTEQEPQAG
                     TLPPVETGKANTNYKPAFEGQTRIAGVKTTTPYEATVIA"
BASE COUNT         6290 a         5320 c         4993 g         5905 t
ORIGIN      
        1 ctctttgaga aagttttcga aaagcgcaat ggattcacta aaaacagaga agttggtttc
       61 gtgcgccagc aacgcatagt taaacatggc ggtttcgcgt acctgcgggt cgaaattatc
      121 gcgcgaagct gcttcaaatg ccatttgtgc ctgctgtttt tgtcctaatt tcaactgggc
      181 ctgcccaatt tgcagctgag cgttttgcgt aagcgcatcc cgttctccaa ctgctttttg
      241 aaacatttgc acagcttcgt tgaatcttcg tgcttccatg taagaaagtc ccatgaaata
      301 ggcatctccc cgaagtggac gggcagtgct gccgaaataa ttttcgtaat agggaatggc
      361 agtagttgct cttccctggc ggtaatggct gttacccaga acccgataca tttcagtatt
      421 gtgctcactg cgcggataag ctttcaggaa tgaatccgcg aggcggatag cttcatccag
      481 tttgttgttg aagaaggtgg attgtgcgga ataaaaagca acctgctctc cgaattcggg
      541 atggttccgc aacccttcaa aacgacgtaa agcggcatcg tagttaccgt tcgtgtaatc
      601 gatatatccg gcgtagaaat ttccggcatt gcggtatttc ttactgttct gcgaaagcaa
      661 tccaaaaagc cggtaggctt catctttatt gtcaagctgt aagtttgcat aagcgctcct
      721 aaagctaaaa tcttcctgct ccgacggttt caggtaatcc aggtcagcct ggtcaaacca
      781 aaaccgggca agcttccagt ctttttcgtc gaaatggtat gaaccgatca gaaatttcac
      841 ctgatgatgg tagatgcttt cggggtattc atccaggtac tccttcattc gctcaccact
      901 gttcttcacc cctttataaa aagacgaaac agccatcatg tattttgctt cttcgatgag
      961 caaacggtcg gtgctttccg tgataaattg cgacaacaaa tcctgggcac ctgtatagtt
     1021 tccttctaga aacatctctt taccctggtt gaaaagcttc ccgggctgat ggtggtagac
     1081 tgtccgctgc gccattgcca ggtgtgttgc aagggcaaaa aatagcacga agattgtttt
     1141 tttcatatat tgctttgtta cactgagatt tctctctgat tttctacttt ctaattctaa
     1201 catcttctaa tattttttca attcctttac ggattgaaat cagtccgaag tcagccggac
     1261 gaagctccct tttatcgata aaaagtagat ccgacacgtc atccatagga gtcatcacac
     1321 tatcatcaag cgacttacac tggaaaaaca gatccatcgt atgcacttca aaacccgaat
     1381 atacgtaaat attcggaatt gaaaaaaggt acttagacga agttatcaac aaccctgttt
     1441 cttcccgaac ttcccgggca atagcctctt cagccgtttc atacatgtct acgaatccgc
     1501 cgggcaggtc gtaagtgccc tttgccggat cttttgctct ccgggcaatt agcacttcac
     1561 ctttctcatt ttcaattatt gccacaacag cagccgatga attaaaataa taggtaaatc
     1621 cgcagtccgt acactttttc gattttaagt tgttttcgga aaagtttcct gaaccgcatt
     1681 cgggacagaa gtgaaattga tgcagggggt gtttcatttc ctcgatatct ccacaatccc
     1741 tgttttcggt aattcacggt tccgtttttc caccgcttcc accacattgt cggcaagccg
     1801 ggcaaaagcc atgccggtga tactgtcgga ctgaagagcc acgggctgtc catcgtcgcc
     1861 accctcacga atggactgta cgatagggat ttgccccaac aatgcatacc cattttgttc
     1921 cgccagttgc ttacaaccgt cttttccgaa gatgtagtat ttattttcag gtagttcagc
     1981 aggggtgaac cacgccatgt tctcgaccaa acccaggatg gggacattca ccttctcacc
     2041 cgtgaacata ctgattccct tacgagcatc ggccagcgcc acttcctgcg gggtacttac
     2101 gataacggct ccggtaatgg gtacggtttg taccagtgtt aaatgaatat cgcttgtccc
     2161 aggcggaaaa tcgatcagga aataatccag ttcgccccag tcagcatcag tgatgagttg
     2221 tttaagtgcg ttgctggcca ttgctccgcg ccaaaccacg gcatcttcct tcttgacaaa
     2281 gaaaccgatg gaaagcattt taaccccata gttctccacg ggaacgatca agtcgcgccc
     2341 ttccattttt tcagtgtaaa cgggcacatc ttcaacacca agcatccggg gaaccgatgg
     2401 cccgaaaata tcggcatcga gcaaccctac tttgtatcct ttttgagcca gtgcaatagc
     2461 taagttagta catacggtac ttttaccgac accgcctttt ccagatgcca cggcaacaat
     2521 gttttttaca tcgggcaaca gatcaggcaa tgcaggccgc ggtgcctgtt tcgactttac
     2581 tgaaatattc cctttgattt ggatcttcgg gtcggcatat gttaaaattg cctgttccgc
     2641 catctttacc agcgatttaa caaacggatc gttctgtctt tcagtgatga gtgagaaact
     2701 cactttcatt ccgtcaatac ggatatcgtc ttccaccatt ccagcagaaa caatatcttg
     2761 ccccgttccc gggtaacgca catttttcag tgcgtcgata attaattgag gatatatagc
     2821 catactattt tgtttaaaat tttatagttg agcatcacgg ccgaaactgc ggtattcgtc
     2881 tttttcaatt tcgatatccg gttccgtcca gttatctttt cttgggcaca ggaaacggat
     2941 gtatttgatg cttaaaccgc gcgaacgcca ttgttgttca taaaaagtct gaatatttaa
     3001 tactttatcg ttagtcccgg atcggtataa atcatcggta tccgctaaag ccgccaggcc
     3061 gttttcttcg atcatggctt tggtatagat gtagaggaaa ttactgtcag ttttgagatg
     3121 aatcacgcct ttatccgcaa gaatttgact gtaggtgttc ataaagcgag tggaagtaag
     3181 ccgtttattg atcttggaca tctgcggatc agggaaagtg atccagattt ccgatacttc
     3241 attttcggca aaaaaatggc gaatcagttc gatatgagtc cgcaagaaag cgacattcgt
     3301 caggttttcg ctcaaagcct gtcttgctcc ggaccacatc cgggcacctt tgatgtcaat
     3361 tcctataaaa tttttctcag ggtagaactg tgccagtcct accgtatatt ctccctttcc
     3421 gcaaccgagc tcgagtacta cagggttatc gtttcgaaaa taatccgttt tccatttccc
     3481 cttaagtggg aaaccgccat tttgtaatgt agagaaagta tattgaaaaa cgttcggata
     3541 ggtcgccata tccgcaaact tcgataattt attttttccc attttcggtt ttctccatat
     3601 ttctatatac aaaaatacaa atttaacttt aaatacgaca atacatcccc gtaaacaacc
     3661 atccatttcc agtatgaaat tgtaagcgat gcaaacaatc tattggcctt atttcaataa
     3721 acttcatcaa tccaaatctt ttttcctgtt atttattatt ttactataca gttgattatt
     3781 aacaaagtaa aacagcctat tttgaaagaa tactttttat cttatctcaa tatttttttt
     3841 tgaaaaaagt gataaagttt caatttatta cttacctttg tcaagcggat tactataaaa
     3901 tactctttat attatgaaag taaatgacat aatgacacaa cttgaggcta agcatcccgg
     3961 cgaggccgaa tatcttcagg cagtaaaaga agttttaact tctatagagg acgtttacaa
     4021 tcaacatcct gaatttgaga aggcaggaat tgtagaaaga attgtggagc ctgaccggat
     4081 tttcactttc agagtgcctt gggtggacga caacggaaaa gttcatgtaa acattggtta
     4141 tcgcgttcag tttaacggag ccataggccc ttataaagga ggtattcgct tccatccttc
     4201 cgtcacgtta tctacactga aattcttagg attcgaacaa acatttaaaa acgcactaac
     4261 tacattacct atgggtggtg gaaaaggcgg atcggacttt tctcctaaag ggcgttctga
     4321 agcggagatc atgcgtttct gtcaggcttt tgtaaccgaa ctctggcgcg atttaggccc
     4381 cgacaccgac gttcctgccg gcgatattgg tgttggtgga cgcgaagtgg gatacatgta
     4441 cggaatgtac aaaaaattag cccgggaaca tacgggaaca ttcactggaa aagggcgttc
     4501 gtttggtgga tcgcgtcttc gtcccgaagc taccgggttt ggggccttgt atttcgtgaa
     4561 tcagatgtgt gagacccaca aaatcgattt gaagggcaaa acggttgctg tttcaggttt
     4621 tggcaacgtt gcctggggag ctacgatgaa ggccaccgaa ctaggtgcca aggtggttgc
     4681 catctcaggc cccgacggct atatctacga tgaagacggt atcaacacac ccgaaaaatt
     4741 caattatatg ctggaacttc gcaattcggg aaacgatgtg gtggcacctt atgccgaaga
     4801 gtacccaaat gccaggttct tcccgggcaa gaaaccttgg gaatgcaaag tggatatagc
     4861 attgccttgt gccattcaga acgaactgaa ccgggaagat gcaaaaagac taaaacaaaa
     4921 tggtgttaca ctcgttgctg aagtttccaa catgggctgt acggccgaag cggttgactt
     4981 ctttatcgaa aacaaaattt tgttcggccc cggaaaagca gtaaacgcag gaggagtagc
     5041 ctgttccgga ttggaaatga cgcagaacgc catgcacatc tcctggacaa acgaagaagt
     5101 ggacaagtgg ttgcatcaga tcatgagcga gatccatgat cagtgtgttg cacacggaaa
     5161 agaggataat tacatcaatt acgtaaaagg tgcaaacatt gccggcttta tgaaggtagc
     5221 cgatgccatg atggctcagg gcgtattata aagatacatc attaatacta tttgaaaacc
     5281 gcagaagagc cgtaaggatt tagccttgcg gcttttttgc gtaaaccggc taaccttttt
     5341 ctattatttc ttatctttgt cttttatcag tgaacggaca ttatcatgag caaaaaatgg
     5401 ctgattgcag tcaccggaac tttcacgcac ttgttgttgg gaactgttta tgcctggagt
     5461 tattttcaaa atcccataag tgaatctgca ggctggagca acgctcagac tgcctgggca
     5521 tttagtttat ccatttttat gctgggagtt acctccgcct gggctggaaa caaaatgtcg
     5581 gtctatggtc cacgcagatt gtcaatgatc ggcggagcgc tttacgcctt tggatacctt
     5641 gtttcgggtt tcgcacttgc ccaccggtac ctgtttttac tctatttcgg gtttggaatc
     5701 atgggaggaa taggattggg attagcttat gttgcccctg tggcaactgt ctctgcgtgg
     5761 tttactcaca aacaggggtt ggcaaccggg atggtagtaa tggggtttgg cttcggagca
     5821 ttggtaatgt ccaagttttt agcccccctt ttacttcaac tatcggatgg gaatttgagt
     5881 actacatttt attatgttgg agcggtaatg atcgtgttga ttcctgttct ggcatcgttt
     5941 ctgcaactac ccaaaccgga taaaacccat gaaaagaagc cggagagaat ctctattgcc
     6001 cggcatatcc aaagcaaacc gtttatcgtt gtctggttaa tttttgcgat aaatattgta
     6061 gcgggaatga tctttatatc ttttcaatca cctctgttac aagatatact aaaaatgcgg
     6121 atgtccgacg gtacaaactt ttctgacggt gaaacgatgg cttctctggc cgctgcgggc
     6181 gctacgctta ttgctgtcag ttctgttttc aacggcatgg gccgatttgc ctggggcagt
     6241 atctccgaca aaataggcag gatgtatact ttccggattc tactggcatt acaggcggtg
     6301 attttcggat tgcttattct cgttcatcat ccggttgttt tctctgttct ggtttgcatt
     6361 gtgttgttat gttacggtgg cgggtttggc gtcttgccat cgttgacaaa agatatgtac
     6421 ggaagtaaat tgatgccttc gctgtatggc gcattcctta ccgcgtggag tatgggcgga
     6481 attattggcc cgcaaatcgt ggctttcatg aaagacaact atgcagagaa ggccggcttg
     6541 tttgcctttg ttgtgggcgg agggctgttg attgtcggtt ttttcaccag ttttttgtat
     6601 cgaagaaaag acgtcataaa tcttgaaaat taaaaaacat gacccccttc ctcctccgca
     6661 tagcacaagc attctattcc aaatacgaaa atgagatata taaactgtcg ttcgttttcc
     6721 cgaacaaacg tgcgggggtt ttctttcaga aatacctggc tgaaattgca gggaaaccgg
     6781 ttttttctcc ccggataatt accatccagg aactgtttga atcgctttcc gcctaccgga
     6841 ccgccgacaa gacagaaatg ctggtaatgc tttatgaaca atacattcgt atcggaaggt
     6901 ctgaagagct ttttgatgat ttcctctatt ggggagagat attgcttaac gatttcaacg
     6961 atgtggacaa acacctggca gatgcccggc aattgttcag aaacatccac aacctgaaat
     7021 cgatggatga cgacctgtcc taccttaccc ctgaacagat taacgctata cgccggtttt
     7081 ggactgcttt tatgccctac gaaggcaatg agaccaagca ggaatttatg gagacttggc
     7141 agattctgtt tgagttgtac acggcctttc gcgaaaccct acacaagaaa ggatacgctt
     7201 acgagggaat gttgttcaga gaggtggcag aaagggcaaa agaaaaagaa gacattgaac
     7261 tgcctttttc tgaactggtt tttgtcggat tgaacgcact cacacctacc gaagtagaat
     7321 tgatgaagta cctaaggaac aggggtgttg ccgattttta ctgggattac gaatctccgt
     7381 tggttcgcga taaaaaaaac cgttcatcgc ggtggattca ggaaaacctg tcgcgcttcc
     7441 cttcaaaact ggaattcggc tcaggcattc cgaatgcgga aaagcccgac ataaacctta
     7501 tcggcattcc ttccggcgtg gggcaggcaa aaactgtgaa ccgtctgctt tcacaactgc
     7561 tcaataacaa gagcattacc gggtccaatg ccggattaaa cacggcgata gtccttcccg
     7621 acgaaaacct gttactgccc gtcctctatt ccatccctca ggaaatcgaa aagataaatg
     7681 tcacgatggg gtattcgttg caacattcat ccgtagcaag cctgacggaa agcattgccc
     7741 ggctgcacca caacacaagg acaatggatg gcgaaacggc gttttatttc cggttcgtac
     7801 tgtcggtgct caatcatccc ctggtgaccc ggatagcgcc atccgaaacc gaggagttga
     7861 aagcctatat ccaacaacac aaccgtgtta tccttacggt ttccgagctt ggcacgcatc
     7921 ccttcctgaa gcttatcttc tcacccataa tggattggac aaaaatcgga gaatacctga
     7981 aatcgatatt atcggaaatt taccgacacc tgacttccga gaagcacccg gatgaagggg
     8041 aatttacggg cgacacacgt tccattgacc ttgaacggga atttattgtc cagtattaca
     8101 aatcgataac ccgcctgcag gatacgttat catctgtgca aaacatgtcg attgacacct
     8161 atttccgttt atttaagcga ttaactgaaa acctcagcgt atcattttcc ggcgagccgt
     8221 tatcgggttt gcaagtgatg ggagtactgg aaacgcgcgt gatagatttc gaaaacctga
     8281 tcattctatc catgaacgaa ggtgtttttc cacttaaaaa cgccaccaac tcttttgttc
     8341 cttacaccct ccgtaaagct tttgacttac caacttacga gcaccaggac agcacatatg
     8401 cttaccattt ctaccggatg ataagccgcg ccaaacgcat tttcctcctc tacgacacac
     8461 gcacagaaga gatgcagacg ggtgaggtta gccgctattt ttatcagttg aaatacttat
     8521 acagccagca ctttcatctg aaagaaagcg tagtttccta caacgtttcg gcccctcatg
     8581 tttctccggt ctcggttgag aaaacacccg aagtgatgcg taagctacag aaatttcgtg
     8641 ccggcggcga cgccagctta tctgcttcac tcatcaacaa ttatatcaat tgccccttgc
     8701 aattttattt ttctgccgtt gaaagactat ccgaagaaaa ggaagtgcag gagtcggtag
     8761 agtccgatgt attcggcacc atgtatcatg ccattatgca gcacctttac aaccgttaca
     8821 ggggcaagtc ggtcctgccg gatacattga acgccataat ccaaaacgat gattacctga
     8881 ccgacctgat tgagcgatct tttgccgaac attatttcaa gcaaaaagat aaaccacgcg
     8941 cactcacggg gcatcatttc ctgatcggag aaattctgcg cgattatgta aagcagactc
     9001 ttcggttcga cacatcgttc acaccgtttg agtatgtgga ttcggaattc gttttcaggt
     9061 caatccacca ggtttcaggt gatttatcgg taaatataaa aggaagtatc gaccggatag
     9121 ataaggtagc cgggagatta cgcattattg attataaatc ggggaaaggc gaattaaatt
     9181 tcaggaatat tgaacagctt ttcgacaaca ccaaagttaa tcgcccctac cagatattgc
     9241 aggtttttat ttatgcactt ttttataaca gtccattacc ggtttctccc gccgtttatt
     9301 acctgaggaa tattttcagg gaacacaacc cggccattac ctttgcaggg gagcccatca
     9361 ccgatatttc ggtttattta tccgcgttta ctgaaaaatt aaatgttttt ctagaagaaa
     9421 tcttcgataa agacattcct ttcgtccaaa cacaaaacga aaaaaactgc gaatggtgcg
     9481 catttaagga tatttgcaga agataacgga aattattctg ttttcctgtc cttttttgga
     9541 ttaaaataac tacttttgcc ctataacact ttctggtttt aaattttaaa ttttaacaaa
     9601 aataaaaaaa acgaaactat tatcgaaagc tatttcgttg atgttggctg gagccatttt
     9661 atttgcaagt tgtagcagca ccacccttat tcaatccagt ccaagcggtg caaaggtgta
     9721 cctcaacggc gagtatgccg gggttacccc atacacacat accgacacca agattgtggg
     9781 aagtagcacg caggtgagat tagaaaaaga aggttatgaa acactgaata ccgctttttc
     9841 cagaaatgaa gatgccgatg tgggagctat cattggtggg attttcttcc tgttcccgtt
     9901 tttgtggacc atgaagtata aacctgtacg cacttacgag ttggttccgt caggaaatgc
     9961 ttcactaaca ggaagcgaca atattacaac tgcacaaaag ggattggtaa aagatgcaaa
    10021 tggaaatgaa tacatttccg gtgtgctgaa agattaatcc tcagtatata cttacaaaag
    10081 aaaagacaat ctcaccgatt gtcttttctt tttctcctct tccacaccgt tgtttccaat
    10141 accctgtttt cggtaatgcg ggaagttttt ctcacggtga gttccatgtc aaacggctca
    10201 cgttccatcc caaaatgatt tccgagtacc gctttcaatc catccagtgt agttacaggc
    10261 tctccgtcct tcttaaatcc acccagccac ttctcgcgct gcgtcctcaa cagatcccag
    10321 ttataagaag atacaataac gagataacct ccatcgttca cccgttcgtg aatgtgcgac
    10381 aaaaacaagc ccggatcact cagttcctcc aacaggttgg gcataacgat aatgtcgtat
    10441 cccgtataat tgggtttcag attcatggca tcggcctgca taaaaagaag gctccctttt
    10501 cctttttcga gtccgaaatc cgataagacc acatcgcgga aatggaccag ttccccttcg
    10561 tctttcatcg tataacgcat gtatccctgt tcctgcaact gaatgggaat acgaatcatg
    10621 cgggcagtga aatccagcgc ggtaacatca ttgaaatact gtgccagttc aaaagccaat
    10681 cgtccggtat cagcgttcaa gtcgagaacc cggctttcgg tttttccttt cgccacatcc
    10741 acaactattt ctgcaagacg tttcgagaaa ttcgtttcct gtgaatacgc atcaccccaa
    10801 ttgttctcgc acgataacgt aacttcttcg tcggtctcgt aatcagcgct ttgaatattc
    10861 agcggagcat ccgattgcac gtaacggaat ccggcatgct gataaaaatg acggcgaaaa
    10921 gcataacgtg aatgcaacgt agcttcattc ccggtagaaa tccatgatcc cccttttatc
    10981 aggttgtgtt taccatcgaa cgtgggggtg gaaaaatcat cgtacaaggg atgtaccttg
    11041 aaaccgggga aaccggtgat tggtgtttcc gtccattgcc acacatttcc cagcacgtca
    11101 taaaaatcgc cctgttggaa tttatccacc gggcagggcg acgtgccata ttcaagatta
    11161 atgtttcccg gcgtagtctc ccaccccatt acgtctttta atcccgaaac ctgatgcaac
    11221 ctgtaccatt cagcttccgt tggcaaccgg taggcctgtc cggttttttc acttttccag
    11281 ttacagaaag cttttgcttc caggtagttt acttcaacgg gccagttcca cggcatcagc
    11341 acctcttcgg caaccaaccg taaccggtat ccgttctcgt cttttcgcca gaaaagcggc
    11401 atctctgctt gtttaaagtt tcgccagctc catccttctt ccgtccagta tttttcagct
    11461 tcataccctc cgtcttccat gaattccaaa aactcccggt tcgaagtcag gtatctggcc
    11521 gctttaaaac cggcaacctc ttcggtataa tctccgtact cgttgtccca cccgtaaaaa
    11581 ggatgatcct tcggtttccc cagtttcatc gtcgcccctt ccacatcgac aagttcattt
    11641 tgcggagcgc ttcccgtctc cgggcaacgt ttgccaaaca aatccgacac caccttatcc
    11701 aacggcaatt gccggatgag taccgacgat gtttccaggt gaatcctttc gtgttcaatt
    11761 cccatcataa ttatccagaa aggactctcc caatcaatag ggagcaacaa gggagccgta
    11821 tcgataatac gaagtatggc ttctttggct ttgtcgcggt aatgacgtac ttcggaaaca
    11881 ggcggccaat cgtaatggtt gttgtcgagg tcgtcccaac tcatctcatc cacgccaatg
    11941 gcaaaaatgg actcaaattt cgggttaatg cgcctatcga taatcttccc caaaaaaagt
    12001 ttattaataa agaacacagc cgtgtgcccc agataaaaca gaataatgtg gcgtaaggga
    12061 tcgccccggt gataaaatac ttcatccgac tttaattgcg tataaagcag ttcgtccaca
    12121 gcccacgttt tcaggaaata ttcccgaatc tcccttctct tttcttccgg cataccggaa
    12181 cgcaggtcaa ttgtttttgt aagtaattct ttcatttttt taaacagatt aatagaacca
    12241 ggaaccgaga ccgcttcgct tttagacttc ttgacttctc ctcttctcaa cttcttaatt
    12301 cctgtcttct tatttctttc gtcatataga tctttctcat ttcaaatttc tcattttgag
    12361 aacgatattt cgtctgtatt atgtccacaa cgggctggaa acccaacttt tcgtacaacg
    12421 acaaggcagg cttattttcc tcccagaccc gtattaccac atccgaatag ttatctttca
    12481 cccgctgtaa aaaactttca aacagttcag aggcaactcc ttttccccga aagtcggaat
    12541 gcgtcattac ctcagtaata taaacggaag tttcaacggg aacctgcgga catctatcac
    12601 ggggaaattc cctgtcaaac gacaacggaa acgcgatcag cacacctacc agtttacctt
    12661 ccgcaaaagc catattcccc caaccgttcc ggagcatctc atccagcgtg ctttcggctg
    12721 attcatgcgg tatatactga gcatgttcgc ctgtcgtaaa agcgtttaaa tacaaaccaa
    12781 ggatcttttc cctgaacttg ggataattga acaaatccaa cccaaaaaca cgaatttgca
    12841 aggcttcttc tttcataaat caacgattga ctgttgaaca aacaaaccag ctaccttgtt
    12901 cgatggatca ccagccgccg actctgccac cgccgccgcc cattccagaa aaaccgccgc
    12961 caaaagaacc gctgctccag tttccaccac ctgagccgga tgtctgcgga ggagttgctg
    13021 cgtgattaac ggtattcgac aaggtgctgt tgagcatatg tccaaaacgg gcaggctgca
    13081 gaaccgttcc cgtataccag ggcggctggt agggttgcgg ctgctgcata gagcttaata
    13141 tcgtgctttc aaacttctcg ccccaaacat cttccatgtc caatgcgatg gcataaggca
    13201 ataactgttc gaaaatttca ggggtgaccg ttggcggatt aaaaaactgc aattgctttt
    13261 cttctgccgt atccatatac attttcaatc cttcaacctg cgattggagg tagagtttcc
    13321 gttcacccgg acgcttaatc agaaagccat atgctataaa gctagtgatt ataagcggga
    13381 ggccaataat aaatccaaca gcgtttaccg acagggaatt tccagaaaag aaaagcaaca
    13441 ggctcaacga cccgacaacc aatgcggctc ccaggacaag accgaaccac cggattctgg
    13501 atttcttcag tattctccgc aaaatcagcg atagaagtgt cacaacggga aaggacacca
    13561 aggcaacggc tataaacgta ccgatctgat ctgcgggctc aaacctcaca aagtacagca
    13621 gagaactgaa atacaggatc agcataatcc agggcagcac cataaattta cggttggcac
    13681 cttctttcaa taccggcttg tattgtttgt tgagatcgtc cctgaacgaa ttcatcaggt
    13741 tcgctatgcc ggaatcatat tccccgtcaa gcaccacttc atcttcatcc ttgaaaaggt
    13801 tctgcagaac cgtgctttct tcccggggaa gcatattatc gtctttcttc gtcctgatga
    13861 gcgtatagac ccggtctttc cgcctcaaga aagatgacct ttcggtttct ttaatgcgca
    13921 aaaatccctt tacggccagg ttcacaatgg agggtgttat caggtcatcc caatattttt
    13981 ctttgtaaag catacctacg cttgcgggag ataatccttc gggcggtgag aattgtggaa
    14041 ttaccacagg cttcggcgga tcaattccgt atttccgcca cgtgaagaaa taataagcaa
    14101 acagcaataa aaaaataagg gcactaacaa tgggcaaccc atttttatcg aagaaagtgt
    14161 ccgggacagg tttggagtat ccgcttttcc gcctctccgg ctgacggaca ataccctttg
    14221 taaatccaac ggagacggtc agcatttcct gcggttccag actgacagct tccaggtaca
    14281 acaacccatc gggcaacgtg tcggccctgc agttactctc cgacgacccg tatctacccg
    14341 tgtaacaacg gtaactcaac actttagcac cttcaggaag tcgaataacg gcgcttacgg
    14401 tatcgagtgg ataatccgac agcgcattta cattccaggt taactcgtcg taaccttcaa
    14461 aaaaaccgat ttgcccggcg gtcttgtagt caagagtata tacatgccaa ccgttatcca
    14521 gaagcacatt cttgttgcca acataaatca ccctgtcact cccttcccgt tcggtaaaaa
    14581 aactttccgg ctccccatcc cttttcacgc tttcgatatc gtaatccaca ggcaccttgt
    14641 ccccgtcctt gtcggatcgg atcaacggca aggcacgggt gatcccacgc ttaaaaatta
    14701 cccctttaac atatacacgc acatcttccc ggacagaaat gtatcctgaa gtatcaaccc
    14761 tcacttcaga acggaacgta tatacctttt cttcctgagc aaaactcgac aaaaagaaaa
    14821 gcgcggtaaa aagaaaacac aaaacgcgtt gcattcagaa tttcacttgt ggagcggttt
    14881 tctcctgttc gttttccaat tcaaagaaaa gagatttggt aaagttgaac atcccggcaa
    14941 ttatgttgct cggaaatgta tcgataacaa tgttcttatc acggacagaa ccgttataga
    15001 accggcgaga cttttcgata tccgattcaa taaggctaag ctggtgctgc aattgcataa
    15061 agttttggtt ggctttcaaa tcggggtatt gttcagcaac agccagcaga ttcatcaatg
    15121 cccggttcag gtcgttctct gccgtttcct gagatttcag gtcttttgcg ctcattgcat
    15181 gggcacgcgc ctgcgtcacc ttattaaagg tctcactctc gtgcgtggca tatcctttca
    15241 ccgtttccac caagtttgga atgagatcgt accgtttctt caactgtaca tttattccgc
    15301 tccacgcctc ttcaacaagt gttctcagct taaccagcct gttgtagatt gaaatgccat
    15361 aaaacagaaa gattaccgcc aaggcaagga ggactattaa aacaacaatt cctgttccca
    15421 taatcgatat aatttaattg gttttaagtt gatgcgataa aggtatataa atttttgtaa
    15481 tctctatcgt taccccaaac gcaagccgga aggaataaat gaacattctc gttaagttaa
    15541 atgggtaaag ccaagcttgc ttgagctgtg gccatagcga gaaaatgtct ccaaatccat
    15601 cccaaaatag gcataaaaca ggacataaaa aaaatcaaaa cagtttctga atgttttctt
    15661 aaaaacaaaa attaattaca cttgttcaac gagtaacagt taaatatttt ttcttaattt
    15721 tgacatctgc agaattgttc accataccac tgagtaaatt cagtagaata gtaaacaaca
    15781 cccaaataaa tttacaaccg aataaaaaac tgaataaccg tatgaaaaat tttatctttc
    15841 gaaaccctac caaactagtt ttcgggaaag gacaaattgc cagattaaat gaactgatcc
    15901 cagctgaggt caacctgatg atcacttacg gaggcggaag cgtcacccga aacggtatat
    15961 atgaccaagt gaaagccgcg ttaaaaggcc gaaaattcat agaattttgg ggaatcgaag
    16021 ccaacccgca cgtggaaacg ttgcgaaaag ccatcgaagt aggaaaaagc gagaacatca
    16081 attacctgct ggccgtaggc ggtggatccg tgctggacgg gacaaaattg attgccgcag
    16141 gtattgcatc ggacgaagac gcatgggaca tcgtgctgaa aggtgttgct gaaaaacaaa
    16201 ttccttttgc atccgtcctt actgtaccgg caacaggatc ggaaatgaac ggtggcgccg
    16261 ttatcacccg cgccgaaacc cgggagaaat ttgctttcgg aggtgattat ccgcagtttt
    16321 ccatcctcga tccggaagta acctactccc tttccaagca ccaggtggct tgcggacttg
    16381 ccgatgccta tacgcacgtg ctggagcaat accttaccac gccgggacaa tcacggctga
    16441 tggacaggtg ggctgaaggg gtattgttgt cgatcatgga gattgctccg aaaataaagg
    16501 aaaacccatg tgattacgac ctgatggccg actatatgct ggcagcgacc ctggctctca
    16561 acgattttat ccggatgggt atttcgcagg attgggcaac gcatcagatc ggacacgagc
    16621 ttactgcgct gcatggcact acgcacggac attcgctcgc gattgtgatg cccggcacgc
    16681 ttcgtgtact gaaagaacaa aaacatgaaa aactcctgca gtacggcgag cgtgttttcg
    16741 gaataacggg gggtagtgaa ccggaacgtg tggacaaagc catcgaaaaa acagaagagt
    16801 tttaccgttc gctgggcctg actacccggt tatcggaaga aaacatcggt aacgaaacca
    16861 tcgaaaccat cgccaaacga tttaacgacc gtggcgtagc gttcggtgaa aaccaaaacg
    16921 tgacgggcga cgtggcacgg gaaattttac tggcgtgctc ataatattat acttaagctt
    16981 cgcaagcttt tatagttgga actaacgctc gattttaacg caagatcacg cggaatgggt
    17041 tcccgcaaaa aaaagctgaa tatctagctg ctagtgtaca agactaagaa actgatgctt
    17101 gcgaaaattg agtaatataa attctgggaa tgattataaa aaattgctgt aattaaaaaa
    17161 tgaaaaaact actcctcttt ttatccgtca gtcttttaat cttcagggct tccgcagtag
    17221 atgatgcacg catgatgcgg tatcccgaca ttaacgggaa cctggttgca tttgtgtacg
    17281 ccggcgatat ctggacggtc caagctacgg gcggcgaagc cagacgactc acctcacatg
    17341 cgggaaccga actttttccg aaaatttctc ccgacgggaa atggatcgcc ttctcggcag
    17401 aatattccgg aaaccgccaa atatgggtga tgcccgctgg aggtggaaca gcacgccagc
    17461 tcacatttta caattccgaa ggcgtgatgc caccccgggg tggattcgac aatgtggtgc
    17521 tcgactggac acccgacagc aaacgtgttc tcttccgtgc caaccgcacc tccttcgggg
    17581 aacgcaacgg caaatatttc acgataagta tcaacggcgg tttggaagag ccgctaccca
    17641 ttgtgaacgg cggttttgcc acattatcgc ccgacggttt gcagctctgc ttcactccgg
    17701 tcgaccggga attccgcacc tggaaacgtt acaaaggggg ccgtgctact gagctgtgga
    17761 catacgacct gacgaaccat acctcacaac agataacgca ttgggctggc agcgaccagt
    17821 ggccgacgtg gcacggagag tatatttttt acgcatccga tcaggacaca cgcctgaata
    17881 tctggcgata caatacgcaa aacaaggaaa acgtgcaaat aacccaccac aaggatttcg
    17941 atgtgatgtg gccatcgggc cgtaacggaa agctggtgta cgagaacggc ggttatctct
    18001 atgtactcaa cctcgcctcg ggaacttccg ataaaatatc cgtttccatc aattacgaca
    18061 accccaacct attgccttat tttaagaacg tgaaagattt cgtgggcagt ttttcgcttt
    18121 caccatcggg gaagcgtgcg ctgttcgatg cccggggtga catcttctcc gttccggtag
    18181 aaaacggtga aatcgaaaac ctcacccgca cacagggcat acgggaaatt ttcccggtat
    18241 ggtcacccga cgggaacaac atcgtttact attccgatgc cactggcgaa tacgaaatgt
    18301 acctgctcga aaacaagaaa ggcgctcaac ccaggcaatt gacaaaaggt tcgaaagcct
    18361 ggaagtatac ggcagaatgg tccccaaaca acaagcaact ggtgtacagc gaccgcacac
    18421 tcaaactttg gctggtggat gccgtttccg gtaaacagac cgtcattgac gaagcatcgg
    18481 cggaagagat ccgtgattat tccttctcgc ccgacggcga ttgggtagcc tacagcaaat
    18541 catcaccaaa ctatcagtcg gcactgtggc tgtaccagat ttctactagc aagaagcatc
    18601 aaatcaccga cgcttcgttc tccgacggac aaccgacatt cagccgcgac ggaaagtttc
    18661 tcttcttcac ctccaacagg gacttcaacc tggctttcag tagtttcgaa ttcgactacc
    18721 tatacaacaa cgcaggacgc atttacgcta taccgttgcg aaacgacggt acaacgctaa
    18781 tccagtacaa aaacgatacg gaacctgttg gcggtgaaaa aaccgattcc gtccaatccg
    18841 ggaacaagaa caaaccgccg ttgaacgttc aaattgacct tgagaacatc cggaaccgta
    18901 tcgtggcgtt acccgttcaa ccggggaatt accgcatcat aggcgcagtg gaggaaggat
    18961 tgctctacac ttccggcaat aagatcatgc gctaccgtat caacgaggaa aaaacggaag
    19021 agatcctcga cggtgcaggc aacggaaccc tgagcgctaa tggaaaatcg ttcatttacc
    19081 ggtccggcag tgattatggc gtagcaaaaa accaggccgg acaaaaagcc ggggctaaca
    19141 aaatcgactt gactaacctg acgatgaaaa ttgagccgcg taaagaatgg gaccagattt
    19201 acaccgatgc tttccgcatt tttcgtgact atttctacgt gagtaacctg cacggagtgg
    19261 attgggaaag gatacaaaag gagtacggag ccctgttgcc acacgttccc agccgctttg
    19321 acctggatta tatcctgaac gaaatcgtga gtgaaaccaa taccggacat gcatacgtgg
    19381 actggggcga cattgccaag gtcgaccgga taaacggtgg attgcttggg gctgaactag
    19441 aagctgacct atccgctaaa cgatatgtca tcaagaagat atacgcaggc gaaaactgga
    19501 atgaaagccg ccgttcgcca cttacagaaa atgggataga cgtaaaagca ggtgactacc
    19561 tgattagtat aaacggtaaa aatctcacta ccgatgacaa tccgtatgaa ttgcttgaaa
    19621 attggggcaa caggcatgtg gagttaacgg taaacagctc tccttcggct tcgggagcaa
    19681 aaacccatac cgtgaaaact attacttccg agcacgaact gcgctatctg gactgggtaa
    19741 atgaacgccg cgccctggtg aataaacttt cgggagggaa aatcggttat atccacgtgc
    19801 ccaacactgc tgtcgagggt aaccgcgagc tcttccgggg gatgtattcc tacaacgata
    19861 aggatgccct cattattgat gaccgctaca acggaggagg gtttatcccc gaccggatga
    19921 tcgacctgct caaccgccgc acactggttt actggcatcg taacgggctt ccccaaccga
    19981 tgaaaacacc cggaatcgct cacgacgggc caaaggttat gctcatcaac ggatactcat
    20041 cgtcaggcgg agatgctttt ccctacttct ttaaaaaaac aggagagggg aaactcatcg
    20101 gcacgcgtac ctggggagga ctggtgggca tttcgggtaa cgcaggcctg gttgacggcg
    20161 gttatatctc cgtacctatt ttcggtattt atgacaatag tggcgagtgg attatcgaag
    20221 gaatcggggt ttatcccgat atcgaggtgg ttgaccgtcc cgaagcgttg gctaaaggta
    20281 acgatcccgg catcgaaaaa gccgtggagg tactgctcaa tgaactgaaa gagaatccac
    20341 gcaaaccagt ttccgtacct gctcctcccg acaggtcgaa atggattgaa acggaaatta
    20401 aatgacaggc tgttccattg aagataagaa agtaaaactt gagtcaaggg gtacaaaatg
    20461 gcaaaagtaa gccttacatg catgtgcatg atccaggatt tgaaaactaa taaagtcctg
    20521 gtacaggaca gggttttaga ctggaaaggg atcagtttcc cggctggcgg cgttgaagaa
    20581 ggtgaaagcc tggtggaagc cgccattcgg gagatgaagg aagagacagg gctaacggta
    20641 tccaacttaa gaccgtgcgg gatcgttcat tggtacaacg ataaaacagg cgatcgccac
    20701 ctggttttca attataaaac aagtgacttc ttcggagagt tgctccctga aacagaggaa
    20761 ggaaaggtct tctgggtaac ccccgaagag ctacccgttt taaatttgtc cgacggattt
    20821 atcgaccggc tgcctatgtt ttttgaggag aaaaacacgg aaaggttcca ggtatggaat
    20881 gaaagcggat acaacccaga catcaaatgg ttttagacga agctatccaa atcaacccct
    20941 gccaacaggt agttaaggtt ttggtgccca tgtcttaaac tactttgtat atttatttgt
    21001 tataacatta aacaaaaact atggacaagc gagaatttat caggaaaagt attgccggag
    21061 cagtagccat cggcacttca ggagtggtct ccacaacgtt tgccgacacc acggccaaac
    21121 cgaagaaaac cggcacaata aaaagaaagc tgggaaaaac cggattcgaa gtttatccgg
    21181 tagtttatgg tggaatcgta tccatgcagg acggacaaaa ggcttcagac aactatgtgg
    21241 cctgggcatt ggacaggggg atcaactact ttgacgtggc tcccagttac ggagatgcgg
    21301 aagaaaagct gggtaattcg ttaaagccct accggaaaga caattttctg gcatgcaaaa
    21361 ccactcaacg attacgtgaa ggtgcggaaa aggagtttga gaaatcactg caattattgc
    21421 agaccgatta ttttgatgta tatcaactac atagcatcag cacgccgaaa gatacggaac
    21481 aaattttcgg accagacggt attatggaga tggtagaaaa agaaaaacaa cgcggacgga
    21541 tccgcaaagt aggtttttcg gcacactcgc aggagcaggc tatccgggcc atgtcgatgt
    21601 acgacttcga cacgatcatg ttccccacca actggcaaat gattaaatta gacggatggg
    21661 gagccggagc tgtaaacgag gcaaaaaggc gaggaatggg cgttttggct attaaaggat
    21721 tgatccaccg ccgttggatg gaagacgaga aacaggactc cagataccag aaatcgtggt
    21781 gtaaacccat cgatgtggaa aaccgggaat ttggtattgc agccctgaaa ttcaccttcc
    21841 aggcaggggc agacgtaatt attccaccgg gcgattttcg taatttctcg ttctgtgtag
    21901 acaatatcga agaaatcctg gacagtcctt taacgaaaaa agaaaaaaca ttgctagaca
    21961 atgaatttct ggccgtaaag gattatccgt tcttcgaccc gaggacataa acaaggaacc
    22021 gttacgaaaa acggctccaa caaaagaatt cgatttcatc agactatttg agtcgccttc
    22081 gctcctcgat tttcaatttt gcgctcggca aatcaaataa atttgctctg ctctcgctta
    22141 acgaaatagt tcatattcat taggcatttt ttcgctatgg caaagcccaa acgagtttgg
    22201 ctttactcat ttagcttaac aaaaatgcgg gtataattta acattaaaga tcacaggtaa
    22261 tgaaacattt taaatttgca gcgataattt cgctggcatg gttttttgcg tcagcctcct
    22321 gcgttgaaca aaaggatagc gatggagggg atatcgacca gacaccgacg gagcaagaac
    22381 ctcaggcggg gacattgcct ccggtagaga cagggaaagc caatacaaat tacaagcccg
    22441 cgtttgaagg gcaaacccgg attgcagggg tgaaaacgac aaccccctac gaagcgacag
    22501 ttatagca
//
LOCUS       DAOSDR010000004        19353 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrimonas sp. isolate AMR_MDS_1826
            NODE_1171_length_19353_cov_4.071614, whole genome shotgun sequence.
ACCESSION   DAOSDR010000004 DAOSDR010000000
VERSION     DAOSDR010000004.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863355
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrimonas sp. (wastewater metagenome)
  ORGANISM  Petrimonas sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Dysgonomonadaceae; Petrimonas.
REFERENCE   1  (bases 1 to 19353)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19353)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:10:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,720
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 35
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19353
                     /organism="Petrimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1171_length_19353_cov_4.071614"
                     /isolate="AMR_MDS_1826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2023866"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..327)
                     /gene="rpsD"
                     /locus_tag="PLI30_00290"
     CDS             complement(<1..327)
                     /gene="rpsD"
                     /locus_tag="PLI30_00290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018667875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HOI78024.1"
                     /translation="MARYTGPKTKIARKFGEPIFGPDKVLSKKNYPPGQHGNTRRRKT
                     SEYGIQLREKQRAKYTYGVLEKQFRLTFEKAARSRGITGEVLLQLLESRLDNVVYRLG
                     IAPTRAA"
     gene            complement(480..869)
                     /gene="rpsK"
                     /locus_tag="PLI30_00295"
     CDS             complement(480..869)
                     /gene="rpsK"
                     /locus_tag="PLI30_00295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005634781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HOI78025.1"
                     /translation="MAKRTVAAKKRNVKVSAEGQAHISSSFNNIIVSLTNDNGEVISW
                     SSAGKMGFRSSKKNTPYAAQMVASDCAKVAYDLGLRKVKAYVKGPGNGRESAIRTIHG
                     AGIEVTEIVDVTPIPHNGCRPPKARRV"
     gene            complement(882..1262)
                     /gene="rpsM"
                     /locus_tag="PLI30_00300"
     CDS             complement(882..1262)
                     /gene="rpsM"
                     /locus_tag="PLI30_00300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009125149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HOI78026.1"
                     /translation="MAIRIVGVDLPQNKRGEVALTYIYGIGRSSANSILAKAEVDRDI
                     KVKDWTDDQAARIREIISTEYKVEGDLRSEVQLNIKRLMDIGCYRGIRHRIGLPVRGQ
                     STKNNARTRKGRKKTVANKKKATK"
     gene            complement(1293..1409)
                     /gene="ykgO"
                     /locus_tag="PLI30_00305"
     CDS             complement(1293..1409)
                     /gene="ykgO"
                     /locus_tag="PLI30_00305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014790280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L36"
                     /protein_id="HOI78027.1"
                     /translation="MKVRASLKKRTADCKIVRRKGRLYIINKKNPKFKQRQG"
     gene            complement(1499..1717)
                     /gene="infA"
                     /locus_tag="PLI30_00310"
     CDS             complement(1499..1717)
                     /gene="infA"
                     /locus_tag="PLI30_00310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017185218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HOI78028.1"
                     /translation="MAKQTAIEQDGTIVEALSNAMFRVELENGHEITAHISGKMRMHY
                     IRILPGDRVRVEMSPYDLSKGRISFRYK"
     gene            complement(1729..2514)
                     /gene="map"
                     /locus_tag="PLI30_00315"
     CDS             complement(1729..2514)
                     /gene="map"
                     /locus_tag="PLI30_00315"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019541319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HOI78029.1"
                     /translation="MNSRTIILKTDEEIELMRQSNRLVGMTLGELSKHIKPGVTTLQL
                     DKIAEEFIRDHGAIPTFLGYGGFPNSICASVNEKVVHGIPNNHPLQEGDIISIDCGTN
                     KQGYCGDSAYTFCVGEVREEIKQLLNVTKEALYKGIEQACHDKRVGDIGFTIQTYCES
                     HGYSVVRELVGHGIGRDMHEAPEVPNYGRRGTGPLLKNGMCIAIEPMINMGSKNVVFE
                     NDGWTVRTKDRKPSAHFEHTVAIRNGKADILSTFEFIQETSNN"
     gene            complement(2536..3858)
                     /gene="secY"
                     /locus_tag="PLI30_00320"
     CDS             complement(2536..3858)
                     /gene="secY"
                     /locus_tag="PLI30_00320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005854004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HOI78030.1"
                     /translation="MGFVQTVKNIWKIEDLRMRLLITVGFIAIYRFGSFVVLPGVNPL
                     QLEALQANASSGLLGLLDMFSGGAFANASIFALGIMPYISASIVMQLLGIAVPAFQKM
                     QREGESGRTKMNQYTRYLTVAILLFQGPAYIYGALGSAIPGGLFDWVIPSTIILAGGS
                     MFVLWLGERITDKGIGNGISFIILIGIIARLPHALVAEFDRLLNAPGGLVLFLLEIIF
                     LMVVIAGAIMLVQGTRKIPVQYAKRIVGNKQYGGVRQYIPLKVNAANVMPIIFAQAIM
                     FIPLTIAQFSVGEGGGFWASLMNYTSFSYNFIFALLIIAFTYFYTAITVNPIQMAEDL
                     KRNNGFIPGVKPGKRTAEYIDLVMSRITFPGSIFLALVAIMPAFAGITGINSQFAQFF
                     GGTSLLILVGVVLDTLQQIESHLLMRHYDGFLKSGAKIKGRSGSIAAY"
     gene            complement(3863..4309)
                     /gene="rplO"
                     /locus_tag="PLI30_00325"
     CDS             complement(3863..4309)
                     /gene="rplO"
                     /locus_tag="PLI30_00325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018667878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HOI78031.1"
                     /translation="MNLSNLKPAEGATKTRKRIGRGSGSGRGGTSTRGHKGQKSRSGY
                     SRKTGFEGGQMPLQRRVPKFGFNPLSRVEYKAINIETLQVLADSQKIEKITPEVLINA
                     GLISRKHLVKILGRGTLSAKLEVEAHAFSKAAEQAISSAGGTAVKL"
     gene            complement(4341..4517)
                     /gene="rpmD"
                     /locus_tag="PLI30_00330"
     CDS             complement(4341..4517)
                     /gene="rpmD"
                     /locus_tag="PLI30_00330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009859531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HOI78032.1"
                     /translation="MATIKVKQTTSRINCPKIQKKTLDALGLTKMNKVVEHEDNPTIR
                     GLIRKVHHLVTIVE"
     gene            complement(4536..5054)
                     /gene="rpsE"
                     /locus_tag="PLI30_00335"
     CDS             complement(4536..5054)
                     /gene="rpsE"
                     /locus_tag="PLI30_00335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018667879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HOI78033.1"
                     /translation="MTGTDRRVKSTNDLELKDRLVAINRTTKVTKGGRTFTFAAMVVV
                     GNEDGIIGWGLGKAGEVTTAIAKGVEAAKKNLMKVPVYKGTIPHEQSARFGGAEVFLK
                     PAFTGTGVKAGGAMRAVLESAGITDILAKSKGSSNPHNLVKATIEALCELRDPITVSQ
                     NRGVSLEKVFNG"
     gene            complement(5061..5405)
                     /gene="rplR"
                     /locus_tag="PLI30_00340"
     CDS             complement(5061..5405)
                     /gene="rplR"
                     /locus_tag="PLI30_00340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005656518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HOI78034.1"
                     /translation="MLTKTERRLKIKTRVRGKISGTKECPRLSVFRSNKQIYAQVIDD
                     INGVTLASASSLKVEEKLPKKEIAAKVGEMIASNAKEAGVEKVAFDRNGYLYHGRIKE
                     LADAARKGGLKF"
     gene            complement(5427..5981)
                     /gene="rplF"
                     /locus_tag="PLI30_00345"
     CDS             complement(5427..5981)
                     /gene="rplF"
                     /locus_tag="PLI30_00345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009859534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HOI78035.1"
                     /translation="MSRIGKLPISLPSGVAVTVGEDNVITLKGPKGELKQHVNPDMKV
                     EVENGVLTVIRPTDDREHRAMHGLYRSLLHNMVIGVSEGFRKELELVGVGYRVTNNGQ
                     ILELSLGYTHNIFLELPQEIKVETKMERNKNPLIILESSDKQLLGQVCAKIRSFRKPE
                     PYKGKGIKFVGEQIRRKSGKTAGK"
     gene            complement(6000..6395)
                     /gene="rpsH"
                     /locus_tag="PLI30_00350"
     CDS             complement(6000..6395)
                     /gene="rpsH"
                     /locus_tag="PLI30_00350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005782213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HOI78036.1"
                     /translation="MTDPIADYLTRLRNAIQAKHRVVEIPASNLKRDITKILFDKGYI
                     LNYKFVEDGPQGSIMIALKYDPVNKVNAIKKLIRISSPGLRRYTGYKNMPRVLNGLGI
                     AILSTSKGVMTDKEAAVQKIGGEVLCYVY"
     gene            complement(6466..6735)
                     /gene="rpsN"
                     /locus_tag="PLI30_00355"
     CDS             complement(6466..6735)
                     /gene="rpsN"
                     /locus_tag="PLI30_00355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004296337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HOI78037.1"
                     /translation="MAKESMKAREVKRAKMVARYAEKRAKYLAEGDYEALQTIPKNAS
                     PVRLHNRCNLTGRPKGYMRQFGLSRIQFREMASKGLIPGVKKASW"
     gene            complement(6739..7389)
                     /gene="rplE"
                     /locus_tag="PLI30_00360"
     CDS             complement(6739..7389)
                     /gene="rplE"
                     /locus_tag="PLI30_00360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HOI78038.1"
                     /translation="MKKEKAAKAAEPKAEVQKEAQAAAPQTEDQGNRSTVTLKKEYGE
                     RIVPALMKEFGYKSVMQAPKLEKIVINQGLGIGVADKKIIETAINELTAITGQKAVAT
                     YSRKDISNFKLRKKMAIGARVTLRHDKMYEFLERLIRVALPRIRDFRGIEGKLDGRGN
                     YTLGIQEQIIFPEINIDSISRLLGMNITFVTSAATDEEGYALLREFGLPFRTEKNK"
     gene            complement(7401..7721)
                     /gene="rplX"
                     /locus_tag="PLI30_00365"
     CDS             complement(7401..7721)
                     /gene="rplX"
                     /locus_tag="PLI30_00365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009132598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HOI78039.1"
                     /translation="MSKLHIKKGDTVYVNSGEDKGKTGRVLEVLVAKNRAIVEGVNIV
                     SKHAKPNAQNPNGGIEKKEASIHLSKLNLVDPKTGKPTRIGRKEDSKGKLVRYAKKSG
                     EVIK"
     gene            complement(7743..8108)
                     /gene="rplN"
                     /locus_tag="PLI30_00370"
     CDS             complement(7743..8108)
                     /gene="rplN"
                     /locus_tag="PLI30_00370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007073026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HOI78040.1"
                     /translation="MIQQESRLNVTDNSGAKEALCIRVLGGTRRRYASVGDVIVVAIK
                     SVIPSSDIKKGAVTKAIIVRTKKEIRRADGSYIRFDDNACVLLNNAGELRASRIFGPV
                     ARELRATNMKIVSLAPEVL"
     gene            complement(8110..8367)
                     /gene="rpsQ"
                     /locus_tag="PLI30_00375"
     CDS             complement(8110..8367)
                     /gene="rpsQ"
                     /locus_tag="PLI30_00375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005853980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HOI78041.1"
                     /translation="METRNLRKERIGVVFSNKMEKTITVTVKWKEKHPIYGKFVSKTK
                     KFHAHDEKNDCNIGDTVRIMETRPLSKTKRWRVVEVIERAK"
     gene            complement(8376..8579)
                     /gene="rpmC"
                     /locus_tag="PLI30_00380"
     CDS             complement(8376..8579)
                     /gene="rpmC"
                     /locus_tag="PLI30_00380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005844133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HOI78042.1"
                     /translation="MKSKKQEYKELSDKDLKERLDAEQADLIQTKIYHTTTPLDNSGS
                     IREKRRNIARIQTELRARELKKA"
     gene            complement(8590..9018)
                     /gene="rplP"
                     /locus_tag="PLI30_00385"
     CDS             complement(8590..9018)
                     /gene="rplP"
                     /locus_tag="PLI30_00385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018358868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HOI78043.1"
                     /translation="MLQPKKSKFRRQQKGRMKGFAGRGNQLAFGSFGIKSLQSKWITG
                     NQIEAARVAVTRYMQRQGQVWVRIFPDKPITKKPAEVRMGKGKGNPEGYVAPVTPGRI
                     IFEIEGVPYDVAKEALRLAAQKLPVTTKFVVRRDYEQPQN"
     gene            complement(9025..9789)
                     /gene="rpsC"
                     /locus_tag="PLI30_00390"
     CDS             complement(9025..9789)
                     /gene="rpsC"
                     /locus_tag="PLI30_00390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019541334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HOI78044.1"
                     /translation="MGQKVNPIANRLGIIKGWDSNWYGGDNYGEMMLEDSKIRTYLNA
                     RLAKASVSRIVIERTLKLVTITICTARPGIIIGKGGQEVDKLKEELKKITDKDVQINI
                     FEIKKPELDAVIVANNVARQVEGKIAYRRAVKMAIASTMRMGAEGIKIQISGRLNGAE
                     MARSEMYKEGRTPLHTFRADIDYAHAEALTKVGLIGIKVWICRGEIYGKKDLAPSFAS
                     SKENRSQGGGGRSDRGNRPEGGRGARRNRRNNNKNA"
     gene            complement(9799..10209)
                     /gene="rplV"
                     /locus_tag="PLI30_00395"
     CDS             complement(9799..10209)
                     /gene="rplV"
                     /locus_tag="PLI30_00395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007662145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HOI78045.1"
                     /translation="MSARKRISAEQRKENRKEVALAVLKNAPSSPRKMRYVADMVRGM
                     EVFKALGVLKFSSKEASRRVEKLLLSAISNWEQKNERKAETGELYIKIISVDEGATLK
                     RLRPAPQGRGYRIRKRSNHVTIVVDTYKKEQEQN"
     gene            complement(10245..10514)
                     /gene="rpsS"
                     /locus_tag="PLI30_00400"
     CDS             complement(10245..10514)
                     /gene="rpsS"
                     /locus_tag="PLI30_00400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007212071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HOI78046.1"
                     /translation="MSRSLKKGPYINIKLEKKVNDMNESGKKAVVKTWARASMISPDF
                     VGHTIAVHNGNKFIPVYITENMVGHKLGEFAVTRTFRGHSGNRKK"
     gene            complement(10536..11363)
                     /gene="rplB"
                     /locus_tag="PLI30_00405"
     CDS             complement(10536..11363)
                     /gene="rplB"
                     /locus_tag="PLI30_00405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008448275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HOI78047.1"
                     /translation="MAIRKLKPTTPGQRHKIIGSFENITASEPEKSLVVGKTSTGGRN
                     HRGKMTIGYIGGGHKKRFRFIDFKRTKDGVPAVVKSIEYDPNRSARIALLYYADGEKT
                     YILAPNGLEVGTTVMSGPEAAPEVGNTLPLANIPLGTVIHNVELRPGQGAKMVRSAGT
                     FAQLVSREDKYAIIKMPSGEVRKILATCKATIGSVGNSDHALQQSGKAGRSRWLGRRP
                     HTRGVVKNPVDHPMGGGEGRASGGHPRSPKGIYSKGLKTRAPKKHSSKYIVERRKKK"
     gene            complement(11371..11664)
                     /gene="rplW"
                     /locus_tag="PLI30_00410"
     CDS             complement(11371..11664)
                     /gene="rplW"
                     /locus_tag="PLI30_00410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005844851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HOI78048.1"
                     /translation="MGVIIKPIFTEKQTAVTEKFENRYGFIVVPGANKVEIKNAIESL
                     YNVHVEQVNTMKYDGKLRSRYTKSGVVTGSTPSYKKAIVTLKKGETIDLFSNI"
     gene            complement(11677..12306)
                     /gene="rplD"
                     /locus_tag="PLI30_00415"
     CDS             complement(11677..12306)
                     /gene="rplD"
                     /locus_tag="PLI30_00415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005853966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HOI78049.1"
                     /translation="MELSIYNINGEVTDKKVTLNDSIFGIEPNDHVIWLDVKHYLANQ
                     RQGTHKSKERNEIAGSTRKLIRQKGSGGARRGDIKSPVMVGGGRVFGPKPRDYGFKLN
                     KKEKALARRSALSLKARNEAIWVVEDFNFDAPRTKNFAELTKKLQLADKKLLFVLPSQ
                     NKNVYLSARNLPGVEIITASDINTYKIMNCGNLVLTESSVAVIDDLLKA"
     gene            complement(12308..12925)
                     /gene="rplC"
                     /locus_tag="PLI30_00420"
     CDS             complement(12308..12925)
                     /gene="rplC"
                     /locus_tag="PLI30_00420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016275270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HOI78050.1"
                     /translation="MPGLIGKKIGMTSVFSAEGKNVPCTVIEVGPCVVTQVKTVENDG
                     YDAIQLGFEDKKDKHTPNGEKGHFKKAGVTPKRHLAEFKGFGNDYKLGSEITVDLFND
                     AVYVDVIGTSKGKGFQGVVKRHGFGGVGQSTHGQHNRLRAPGSIGACSYPAKVFKGTR
                     MGGQMGNERVTVQNLQVIKVIPEHNLLLLKGSIPGCKGSIVAIEK"
     gene            complement(13030..13335)
                     /gene="rpsJ"
                     /locus_tag="PLI30_00425"
     CDS             complement(13030..13335)
                     /gene="rpsJ"
                     /locus_tag="PLI30_00425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005782187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HOI78051.1"
                     /translation="MSQKIRIKLKSYDYSLVDKSAEKIVKTVKATGAVVSGPIPLPTH
                     KRIFTVNRSTFVNKKSREQFELSSFKRLIDIYSSTAKTVDALMKLELPSGVEVEIKV"
     gene            complement(13351..15479)
                     /gene="fusA"
                     /locus_tag="PLI30_00430"
                     /pseudo
     CDS             complement(13351..15479)
                     /gene="fusA"
                     /locus_tag="PLI30_00430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005853959.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
     gene            complement(15505..15981)
                     /gene="rpsG"
                     /locus_tag="PLI30_00435"
     CDS             complement(15505..15981)
                     /gene="rpsG"
                     /locus_tag="PLI30_00435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019541343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HOI78052.1"
                     /translation="MRKTKPKKRQVLPDPVYGDVRVTKFVNHLMYDGKKSISYDIFYS
                     ALDTVKAKLPNEEKPALEIWKAALDNITPQVEVKSRRVGGATFQVPTEIRPERKESIS
                     MKNLILYARKRGGKTMADKLAAEIVDAYNNQGGSYKRKEDMHRMAEANRAFAHFRF"
     gene            complement(16147..16554)
                     /gene="rpsL"
                     /locus_tag="PLI30_00440"
     CDS             complement(16147..16554)
                     /gene="rpsL"
                     /locus_tag="PLI30_00440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007212064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HOI78053.1"
                     /translation="MPTIQQLVRKGRTVIEEKSKSRALDSCPQRRGVCVRVYTTTPKK
                     PNSAMRKVARVRLTNTKEVNAYIPGEGHNLQEHSIVLVRGGRVKDLPGVRYHIVRGTL
                     DTAGVNGRTQRRSKYGAKRPKPGAVAKPGAKKK"
     gene            16875..17384
                     /locus_tag="PLI30_00445"
     CDS             16875..17384
                     /locus_tag="PLI30_00445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005656029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HOI78054.1"
                     /translation="MSHDKFHRIILPLKDKLFRLAWSIVRDSAEAEDIVQDVFLKLWS
                     KNNEWENIENLEAYCFRATKNLALDRVESLSIRRTENIAPELENGIFTDHVTPFFKLV
                     ETERNTLIYRCINQLSESQKAVFQLREIEGMSYKEIAQVLDISEDLVKVNLFRARNKL
                     KELLSGKQE"
     gene            17381..17944
                     /locus_tag="PLI30_00450"
     CDS             17381..17944
                     /locus_tag="PLI30_00450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI78055.1"
                     /translation="MNNERIHTLLENYWNCETSVSEERELQEFFNFGDVPDDLLPYTP
                     LFAYKQNHQAMKLSCDFEKKLDTLIRRTDRQKQYITIKIFEPFLRIAASVILIAGIGV
                     SVYFVARQNNRVFAETYNDPRAAIQQATSALEKLSKALRATEEASLKSLEQLDDLDLD
                     WSEIDSLSTRIPLYDPDLSKNIKPENL"
     gene            17941..18468
                     /locus_tag="PLI30_00455"
     CDS             17941..18468
                     /locus_tag="PLI30_00455"
                     /inference="COORDINATES: protein motif:HMM:NF025426.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4252 domain-containing protein"
                     /protein_id="HOI78056.1"
                     /translation="MRKIFLVLFTLFLATSTFANDFITRFLKECVETERPVSNVNIGK
                     AMLNKMASNTSDEELKTTFRELNSIRIITTENKADSKFYFEKAKEMASSEFTDYKEVV
                     SVNERRSKINILMKKTDEKTQDLILMALDDDSKLTIITVSGNIDFNSISKISDSLRKG
                     EAEEPVLEKGNEKNN"
     gene            18684..>19353
                     /locus_tag="PLI30_00460"
     CDS             18684..>19353
                     /locus_tag="PLI30_00460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006844050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase domain-containing
                     protein"
                     /protein_id="HOI78057.1"
                     /translation="MYNKSLHLVLEDGTVFQGKSFGYEAPVAGEVVFSTGMVGYTESL
                     SDPSYLGQILTLTYPLIGNYGVPKDESHQGISTFYESERIQASGLIVSDFSFEYSHWN
                     AAKSLSDWLKENKVPAVYGIDTRELTKLVREKGTMLGKLVFPGEPDIPFVNPDDKNQV
                     AKASCKEVIVYGSGKHKVILVDCGVKNNIIRCLLKRDTTIVRVPWDYDFNEMEFDGLF
                     ISNGP"
BASE COUNT         4896 a         4545 c         3755 g         6157 t
ORIGIN      
        1 agctgctctt gtaggtgcta tacctaatcg ataaacgacg ttgtctaaac gcgattccaa
       61 taactgaagc agtacttcac ctgtaatacc gcggctacgg gcagcttttt cgaaagttaa
      121 ccggaattgt ttttccaata ctccgtaagt atattttgca cgttgttttt cacgcaactg
      181 aattccgtat tccgacgttt tacgtctacg tgtatttccg tgctgtcccg gggggtagtt
      241 ctttttagaa agaaccttat cgggaccgaa aataggttca ccgaattttc gggcgatttt
      301 tgtttttgga ccagtatatc ttgccatttt tacaaattaa actttttcta ctgaagccta
      361 aactgtgcag ccgcgattac agagaagtat attgtaatca aatcacagct caagtttctg
      421 tatgattgaa acttatgatt atttactttt ttttgatgtt cagacgaata actaccggat
      481 cagactcttc tagctttggg tggacgacag ccattgtgcg gaatgggtgt aacgtctaca
      541 atctcggtca cctcaattcc tgcaccgtga atggttctga tagctgactc gcggccgttt
      601 cccggacctt tcacataagc tttcacctta cggagcccca ggtcataagc cactttagcg
      661 cagtctgaag ccaccatttg agccgcgtaa ggggtgttct ttttagaact tctgaagccc
      721 atttttccgg ctgatgacca gcttatcact tctccgttat cgttggttaa tgaaacaatg
      781 atgttattga acgatgaaga gatgtgagcc tgtccttcgg cacttacttt aacgtttctc
      841 ttttttgctg caactgttct ttttgccata ttccttatct gttatttagt agcttttttc
      901 ttattagcaa cggttttctt tcttcctttt cgcgtacgtg cattgttctt ggtgctttgt
      961 ccgcggacag gtaacccgat acgatggcgg ataccacggt agcagccgat atccatcagt
     1021 cgtttaatgt tcaactgtac ttcagagcga agatctcctt ccactttgta ttcggttgaa
     1081 ataatctcac gaatacgtgc tgcctgatcg tctgtccaat cttttacttt gatatctcta
     1141 tccacttcgg ctttagctaa aatactgttt gccgagctgc gtccgatgcc atagatataa
     1201 gttaacgcta cctcgcctct cttattttgc ggcaaatcga cccctacaat tcttatagcc
     1261 atataatttt tttaaattgt tattttttaa tcttatccct gacgttgttt gaacttggga
     1321 ttctttttgt tgataatgta taaacggcct tttctgcgaa caattttaca gtcagccgta
     1381 cgttttttta aagatgctct tactttcata tttgtatttt ttagatgtca gacatcagat
     1441 tttagatttt agacaattat ggtccgacgt ctattatctg atatctaatt ttcttgtttt
     1501 atttatatct gaatgaaatc cttcctttcg aaagatcgta aggcgacatt tctactcgca
     1561 ctctgtctcc gggtaaaatc cggatgtaat gcatacgcat tttccccgag atgtgtgcgg
     1621 taatctcatg tccgttttct aattctacac ggaacatagc attagacaat gcttctacta
     1681 ttgttccgtc ttgttcaatt gctgtttgtt tagccatttc cgctcttttt aattgttgct
     1741 tgtttcctga ataaactcaa atgtcgacaa aatatcggct ttcccgtttc ggatagctac
     1801 agtgtgttca aaatgtgccg atggtttgcg gtccttagtt cgaaccgtcc atccgtcgtt
     1861 ttcaaacacc acatttttac ttcccatatt gatcatcggt tcaatggcaa tacacattcc
     1921 gtttttcaat aacggacctg tccctcttct accataattc ggcacttcgg gcgcttcgtg
     1981 catgtctctg cctattccgt gacctaccaa ttcccttaca accgaataac cgtgtgactc
     2041 acaataagtt tgtatagtga aaccgatgtc tcccactctc ttatcgtgac aggcctgttc
     2101 gatgcctttg taaagcgctt ctttggtaac gttaagtaat tgtttaatct cttctcttac
     2161 ctctcccacg cagaacgtgt atgccgaatc gccacaatat ccctgtttgt ttgtaccgca
     2221 atcaatggat atgatgtcgc cctcttgcaa agggtgattg ttgggaattc cgtgcaccac
     2281 tttttcattt accgaagcac aaatggaatt aggaaaaccg ccgtatccta aaaaggtggg
     2341 gatagctccg tgatccctga taaactcctc cgcaattttg tcgagttgaa gtgtcgtcac
     2401 cccgggtttg atgtgtttgg aaagctctcc cagagtcatt cctaccaaac ggttgctttg
     2461 acgcatcaat tcgatttctt cgtcggtttt cagtatgatc gttctactat tcatccccat
     2521 ttattcgcca gaaacttaat aagctgcaat tgagccggac cggcctttaa ttttagctcc
     2581 cgatttaagg aaaccatcgt aatgacgcat cagcaagtga ctttctatct gttgcaaggt
     2641 gtcgagcact actccaacaa gaatcagcaa cgaggttcct ccgaaaaatt gtgcaaactg
     2701 cgaatttatc ccggtaattc cagcaaaagc aggcataata gctactaatg ccaagaaaat
     2761 tgacccagga aacgtgatcc ttgacatgac aagatcgatg tactccgccg tccttttacc
     2821 gggttttaca ccgggaataa agccgttgtt tctcttcagg tcttctgcca tttgaatagg
     2881 attaacggta attgccgtat agaaataggt gaaagcaata atcagcaacg caaagatgaa
     2941 attatacgaa aaactggtgt aattcatcag cgaagcccaa aaaccgccgc cctctcctac
     3001 tgagaactga gcaatagtaa gcggtataaa cataatcgcc tgtgcaaaga tgataggcat
     3061 tacgttagct gcattcactt ttagagggat atactggcgc acaccaccgt attgcttgtt
     3121 acccacaata cgtttggcat actgtacagg tatctttctc gtgccttgta ccaacataat
     3181 cgctccggct ataaccacca tcaggaaaat gatttcgagc aggaagagca ccaatccccc
     3241 gggagcattg agcaaccggt cgaactcagc gaccaaagca tgcggcagac gggcaataat
     3301 accaatcagg atgataaagg aaataccgtt accaatacct ttgtcggtaa ttctttctcc
     3361 cagccacagc acaaacatac tgccaccggc gagaataatg gttgaaggaa ttacccagtc
     3421 aaacaatcct cccggaattg ccgatcccaa agctccgtag atataagcgg gcccttgaaa
     3481 aagcaaaatg gcaaccgtca gataacgggt atactggttc atcttcgtac gtccactttc
     3541 tccttcgcgt tgcattttct ggaaagccgg aacggcaata cccaacaact gcatcacgat
     3601 ggaggcagag atgtagggca taattcccaa cgcaaaaatt gaagcgttcg caaacgcacc
     3661 tcccgagaac atatcgagca atcccagcag acctgagctt gcattggcct gtaaggcctc
     3721 gagttgcagc gggttaacac ccggaagtac gacaaatgaa ccgaaccggt atatggctat
     3781 aaaacccacc gtgatgagta acctcatccg caggtcttca attttccaaa tattttttac
     3841 tgtctgtaca aatcccattt tttcagagtt ttacggctgt acctcctgca gaagaaatag
     3901 cttgttccgc agccttggag aaagcgtgag cttcaacttc caattttgcc gaaagtgttc
     3961 ctcttccaag aattttaacc aagtgttttc gggaaataag gccggcattg atcaatacct
     4021 ccggagttat cttttctatt ttctgagaat cggccaatac ctgaagtgtt tcaatgttga
     4081 tggctttgta ttcaactctg ctcagtggat taaatccgaa tttaggtacg cgacgctgta
     4141 aaggcatctg tcctccctca aatccggttt ttctggaata acccgatctt gatttttgtc
     4201 ctttgtgtcc tcttgtcgaa gtacctcctc tgcccgatcc cgatccgcgt ccgattcttt
     4261 tgcgggtttt ggttgcgcct tcagcgggtt ttaagttcga taaattcatt attatctata
     4321 ttttatcgtt ataaactaat ttattctaca atcgttacta aatggtgaac tttacggata
     4381 aggcctctaa tagtagggtt atcttcatgt tcaaccactt tgttcatttt cgtcaatcct
     4441 aacgcatcta acgttttttt ctgaatttta gggcagttta ttctgctggt agtctgtttt
     4501 acttttatag tagccatttc aaaatccttc cgtttttatc cgttaaatac tttttctaaa
     4561 ctaactccgc gattttgaga aacagtaatg ggatctctca gttcgcacaa cgcttcaatg
     4621 gtggctttta ccaggttgtg aggatttgac gatcctttcg atttcgccag aatatcggta
     4681 attcctgcac tttccaacac ggcacgcatg gccccccctg cttttactcc tgttccggta
     4741 aaggctggtt tcagaaaaac ttcggctccg ccgaaacggg cagattgttc gtgagggata
     4801 gtccctttgt aaacgggaac tttcatcaag ttctttttgg ctgcttcaac acctttagca
     4861 atagcagtag tcacctcacc ggctttaccc aatccccagc caataatacc gtcttcgttt
     4921 ccaactacaa ccatagcagc aaatgtgaat gtacgacctc cttttgttac cttggtagta
     4981 cgattgatag ccactagcct gtccttcaac tctaaatcgt ttgtcgattt tactcttcta
     5041 tctgttcctg tcatagttgt ttaaaattta agtcctcctt tacgagcggc atcagccaat
     5101 tcttttatac gtccatgata taagtaaccg tttctgtcga atgctacctt ctcaaccccg
     5161 gcttctttag cgttgctggc aatcatctcg ccaacttttg cagctatttc ttttttggga
     5221 agtttttctt ccactttcaa agatgaagcc gatgccagcg taactccatt aatatcatcg
     5281 ataacctgcg cgtatatttg tttattactc ctgaacacgg acaaacgcgg acactccttg
     5341 gtcccgctga tcttaccgcg aacacgagtc ttaatcttta gtcttctttc tgtttttgtt
     5401 aacatagcga tttctgttta cgtaaattat ttacctgctg tcttacccga tttgcgacgg
     5461 atttgttcac caacaaattt aataccttta cctttgtaag gttcgggttt gcggaatgaa
     5521 cgaatttttg cacaaacctg tccgagcaat tgcttatcgg aagactccaa aataataagc
     5581 ggattcttgt tcctttccat cttggtttcc actttaatct cctggggtaa ttccaggaat
     5641 atgttgtggg tataaccaag tgataattcc agaatttgtc cgttattggt tacacggtat
     5701 ccaacaccaa caagctccag ttccttgcga aatccttccg aaacgccaat caccatattg
     5761 tggagcaaag aacggtataa cccgtgcatg gctctgtgtt ctctatcgtc ggtaggcctg
     5821 ataacggtta agacaccgtt ttcaacctct actttcatat cagggttcac gtgttgtttt
     5881 agttctcctt tagggccttt caacgtaatc acgttatctt ctcccaccgt tactgcaacg
     5941 ccggaaggca atgaaatggg caattttcct attcttgaca tactattctc ctcctgaaat
     6001 taataaacgt aacacaatac ttcaccgccg attttctgaa cggcagcttc tttatccgtc
     6061 attactcctt ttgaagtaga taatatggca atacccaaac cgttcagtac ccggggcata
     6121 ttcttataac cggtataccg acgtaacccg ggtgaagata tgcgaatcag ttttttgatc
     6181 gcattcactt tatttaccgg atcgtatttc aaggcaatca taatagagcc ttgcggtccg
     6241 tcctctacaa acttataatt aaggatgtag cctttgtcaa agagaatctt tgtgatgtct
     6301 cttttcaagt ttgatgcggg aatttctacc acacgatgct tcgcctggat agcatttctc
     6361 aaccgcgtca aataatctgc tattggatct gtcattataa aaaaatttaa attgatcccg
     6421 actgtcggga caatatttaa taaaacttcg ttattgcact gtattttacc agcttgcttt
     6481 tttcactccg ggtataagtc ctttcgatgc catttccctg aactgaatgc gtgataatcc
     6541 aaactggcgc atatagcctt tgggacgtcc ggttaaattg caacggttgt gcaaacggac
     6601 gggagacgca tttttaggga tggtttgaag tgcttcgtaa tcaccttcag ccaaatattt
     6661 tgctcttttt tcggcataac gggcaaccat tttagctctt ttcacctcgc gggctttcat
     6721 tgattctttt gccatttctt acttgttttt ttctgttcta aacggtaatc caaattctct
     6781 gaggagagca taaccttctt cgtcggttgc tgcagaggta acgaacgtaa tgttcattcc
     6841 cagcaaacgg gagatgctgt cgatattgat ttcaggaaag ataatctgtt cctggatccc
     6901 cagggtgtag tttcctcttc catcaagttt cccttcaata cccctgaagt cacgaatacg
     6961 tggcaacgca acgcggataa gtctttcgag gaattcatac attttatcgt gacgcaacgt
     7021 tacacgagct ccgatagcca ttttcttacg taacttgaaa ttggagatat cttttctcga
     7081 ataggttgcc actgctttct gaccggtgat tgccgttagt tcgttgattg ccgtttcgat
     7141 aatttttttg tcagccactc ctatacccaa tccctggttg ataacaattt tttcgagctt
     7201 gggagcctgc ataacggatt tgtatccaaa ttccttcatt agggcaggca caatacgttc
     7261 cccgtattct tttttgagag taaccgtact tctgtttccc tgatcttctg tttggggagc
     7321 agcagcctga gcttctttct gaacttcagc tttgggttca gcggcttttg ccgctttttc
     7381 ttttttcact tctttagcca ttacttaatt acctcccctg atttttttgc ataacgtact
     7441 aacttacctt tactgtcttc ttttcttcca atgcgcgttg gtttaccggt tttcggatca
     7501 accaaattta gtttcgagag atgaatggaa gcttctttct tttcgatacc tccattggga
     7561 ttctgtgcat tgggttttgc atgcttcgac acgatattta ctccttctac aatggcgcgg
     7621 tttttagcca ccagtacctc gagcacacgt cctgttttgc ctttgtcttc accggagttc
     7681 acgtaaaccg tatctccttt tttaatatgt aatttactca ttgtctgtga ttgtttaata
     7741 gattaaagta cttcgggcgc taatgaaaca atcttcatgt ttgtagcacg taattcacgt
     7801 gcaaccggcc caaagatacg gctggctctc aactcaccgg cgttgttcag aaggacgcag
     7861 gcattgtcgt cgaaacgaat ataagaacca tcagcacgac gaatttcttt tttcgtacgt
     7921 acaatgattg cttttgttac agcgcctttt ttaatgtcac tcgaagggat tacgctcttg
     7981 atagccacaa caattacatc cccaacagac gcgtagcgtc ttcttgttcc acctaataca
     8041 cggatgcaaa gggcttcctt ggctccgcta ttgtcggtca cgtttagtct tgattcttgc
     8101 tgtatcatct tacttagccc tttctattac ttcaaccact ctccatctct ttgttttgct
     8161 caaaggacgt gtttccatta tacgtactgt atcccctatg ttacagtcat tcttttcatc
     8221 atgggcgtga aattttttcg ttttactaac gaacttccca tatatagggt gtttttcttt
     8281 ccattttaca gtaacggtga tggttttttc catcttgttg ctgaaaacga ccccgattct
     8341 ttcttttctt aaatttctcg tttccattat aattattaag cttttttcaa ttcacgagcg
     8401 cgcaattcag tctgaatacg tgcaatgttt ctgcgttttt ctcttatcga acctgaattg
     8461 tcgagcggag tagttgtatg ataaatcttg gtctgaatca ggtccgcctg ttcagcatcc
     8521 aacctttcct tcaaatcctt atccgataac tccttgtatt cttgtttttt tgatttcatg
     8581 ccttacgaat taattttggg gttgttcata atctcttctg acaacgaatt ttgtcgtaac
     8641 aggcaatttc tgtgctgcca ggcggagagc ttcttttgcg acatcgtacg gtacaccttc
     8701 gatttcgaaa ataattcttc cgggagtaac aggagccacg tatccttcgg gattaccttt
     8761 tcctttaccc atacgtactt cagcaggttt tttggtaatt ggtttatcgg ggaagatgcg
     8821 tacccaaact tgtccttggc gttgcatata gcgtgttacg gcaacacggg cagcctcaat
     8881 ctgattgcca gtgatccatt ttgattgtaa cgattttatc ccgaacgaac cgaatgccaa
     8941 ctgattaccg cgaccggcga agcctttcat gcggcctttc tgctgtcttc taaattttga
     9001 ttttttcggt tgtaacatat tggtttatgc gtttttgtta ttatttcttc tgtttcttct
     9061 ggcacctctg cctccttcgg ggcgattacc tctgtcgctg cgtccgccac cgccttgtga
     9121 acgattttcc ttggaagatg cgaatgaagg tgccaggtct tttttaccgt agatctctcc
     9181 tctgcagatc caaaccttga taccgatcaa tccaactttg gtcaacgctt ctgcatgagc
     9241 ataatcaatg tcggcacgga aagtatgcaa cggagtcctt ccctctttat acatttccga
     9301 ccgggccatt tcggcaccgt tcaagcgacc tgagatttga atctttatgc cttctgcgcc
     9361 catcctcatg gtagatgcga tggccatttt aaccgctctg cggtaagcaa ttttaccttc
     9421 cacctggcga gcaacattat tggccacgat aaccgcatcc agctcgggtt tctttatctc
     9481 gaagatattg atttgaacat ccttatcggt tattttcttt aactcttctt ttagcttgtc
     9541 cacttcctgg ccacctttac cgataatgat tcccggacgg gcagtacaga tagtgatggt
     9601 tactaatttc agcgttcttt caatgacaat gcgagaaaca cttgctttgg ccagacgagc
     9661 attcaaataa gtgcgaattt tgctgtcttc cagcatcatt tcgccataat tatctccacc
     9721 gtaccaatta gagtcccatc ctttgatgat tcccaaacga tttgctatcg gatttacttt
     9781 ttgtcccatc tgcctgtatt aattttgttc ttgttctttt ttataagtat ctaccacaat
     9841 tgtcacgtga tttgaacgtt tgcggattct gtagccgcgg ccttgcgggg caggacgaag
     9901 tctcttcagc gttgcacctt cgtctacaga aattattttg atataaagtt cgcctgtttc
     9961 ggctttacgt tcgtttttct gttcccagtt agagatggcc gagagtaata atttttcaac
    10021 tcttctcgat gcctccttgc tggaaaactt caaaacgccc aatgctttaa aaacttccat
    10081 tccgcgcacc atatctgcaa cgtatcgcat tttccggggc gaagaaggag catttttcaa
    10141 cacggctaaa gcaacctctt tgcggttttc tttgcgttgt tctgctgata ttctttttct
    10201 agcactcatg tcttttcttt tttttattca gttaagcccg ccgattattt ctttctgttg
    10261 ccggaatgtc cacggaatgt gcgcgttacc gcaaattcac ctaacttatg tcctaccatg
    10321 ttttcggtaa tatacaccgg aataaattta tttccgttgt gtacagcaat agtgtggccc
    10381 acgaaatcgg gggaaatcat tgaagcacgt gcccatgttt ttacgacagc ttttttgccg
    10441 ctctcgttca tgtcattcac tttcttctct aattttatgt tgatatacgg accttttttt
    10501 aatgatctgc tcataattct tacttgttaa tcaggttatt ttttctttct tctttctacg
    10561 atgtatttag aagaatgctt tttcggagct cttgttttca agccctttga ataaatacct
    10621 ttgggcgaac gtggatgacc tcccgacgcg cggccctcac caccacccat ggggtggtca
    10681 accgggtttt taacaacccc gcgtgtgtgt ggacgacgtc caagccatct tgaacgaccg
    10741 gctttacctg attgttgcag agcatggtct gaattaccaa cacttcctat agtagcttta
    10801 catgtagcaa gaattttacg aacttcacct gaaggcattt tgataatcgc gtatttatct
    10861 tcacgcgaaa ccaactgagc gaatgttccg gccgaacgca ccatttttgc accttgtccc
    10921 gggcgcaact caacgttatg aatcaccgta ccaaggggta tgtttgccaa aggcagtgta
    10981 ttgccaactt cgggagcagc ttccggtccc gacatcactg tggttccaac ttctaatccg
    11041 ttgggagcaa ggatatacgt tttttctcca tcggcataat aaagcaacgc gatgcgtgca
    11101 gaacgattgg gatcgtattc aatgcttttt acaacggcag gaactccgtc ttttgttctc
    11161 ttgaaatcaa taaaacgaaa acgttttttg tgtccgccac caatgtaacc tatcgtcatt
    11221 ttcccgcgat ggttgcgtcc acctgtactt gttttaccaa ctacaagaga tttttccggt
    11281 tctgatgcag tgatattttc aaaagaaccg ataatcttgt gtctttgccc cggtgttgtg
    11341 ggctttagtt tacgtattgc cattttttaa ttagatattg ctgaataaat cgattgtttc
    11401 accttttttc aaggttacaa ttgctttttt ataagatggc gtgctaccgg taacaacacc
    11461 ggattttgtg tagcggcttc tcagcttgcc atcgtatttc attgtattca cctgttctac
    11521 gtgaacattg tagagcgatt caattgcatt tttgatttct actttgttag ctccgggaac
    11581 tacaatgaaa ccgtaacggt tttcgaactt ctcggtaacc gcggtttgtt tttctgtaaa
    11641 aattggtttt ataattactc ccattgtttt gtctccttat gctttaagta aatcatcaat
    11701 taccgcaaca gacgattccg tgagcactaa attcccacag ttcataattt tgtatgtatt
    11761 tatgtctgaa gcagttataa tctcaactcc cggtaaattt cgagccgaca aatatacgtt
    11821 tttattttga cttggcaaaa cgaaaagtag ctttttatca gccagttgca gtttttttgt
    11881 tagctcagca aagttcttcg ttctgggtgc gtcgaagttg aaatcttcca ctacccaaat
    11941 agcttcgttt ctggctttta atgaaagtgc tgaacgacgt gccaatgctt tttccttttt
    12001 gttgagctta aagccgtaat cacgtggttt aggaccaaag acccggcctc cgcctaccat
    12061 aaccggcgat ttgatgtcgc cacggcgtgc accaccactc cctttctggc gaatgagctt
    12121 gcgggtgctt ccagctattt catttctctc tttcgatttg tgcgtaccct gacgctggtt
    12181 agccaaataa tgttttacgt ctaaccagat aacatgatcg ttaggttcaa ttccgaaaat
    12241 ggaatcattg agtgtcacct ttttatctgt tacctctccg ttgatattgt atatgcttaa
    12301 ttccatttta tttttctatt gctacaattg aacctttaca tcccggaatc gaacctttca
    12361 acaataaaag attgtgttcg ggaattactt taatcacctg aagattttga acggttaccc
    12421 gctcattacc catctggccg cccatacgag tacctttaaa tactttcgcg ggatacgaac
    12481 aggctccgat agaacccggg gcacgcaaac ggttatgctg cccgtgtgtg gactgtccta
    12541 caccaccgaa accgtggcgt tttactacac cttgaaaccc ttttcctttc gatgtcccga
    12601 tcacgtccac gtaaacggca tcgttaaaaa gatcaacagt gatttcggac cccaacttgt
    12661 agtcgtttcc aaatcctttg aactcggcca agtgtctctt cggggtaact ccggcttttt
    12721 taaaatgacc tttttcgccg ttgggtgtgt gtttgtcttt tttatcttca aacccaagtt
    12781 gaatagcgtc gtaaccgtca ttttcaacgg tctttacctg agtaaccacg caaggaccta
    12841 cttcgataac agtgcatgga acatttttcc cctcggcact gaaaacggat gtcattccga
    12901 ttttttttcc aattaatcct ggcatttctc tgttgtttaa ttaattaatt atttagattc
    12961 aagaaccaag agccaaagat ttttatcttg gttcttctgt cctggctcct ggctcttttg
    13021 tcttatttat tacactttga tttctacttc taccccactc ggcaattcaa gcttcatcag
    13081 cgcgtcaacg gtttttgccg ttgagctgta gatatcgatc agccgcttga aagacgagag
    13141 ttcgaattgc tcgcgcgatt ttttgtttac gaatgtagag cggttcacag taaatatgcg
    13201 tttgtgtgtg ggcaacggaa tgggtccgct tactactgca cccgtagctt ttacggtttt
    13261 tacgattttt tctgcagatt tatccaccag gctgtaatcg taggacttca gtttaattct
    13321 gattttttgg ctcatattat ttttataatg ttatttaatc aaatcaacac gaccttttac
    13381 ttccgtcagc acttgtcttg caatcgaggg agaaacttcc tcaaaatggt cgaaagacat
    13441 tgtggaggtt gctcttcccg aagtatggtc cgcaatgaag ttacatatcc gaacatttcc
    13501 gaaagcggag tttttgcctt tacaatgcgt gcacccgacc ggttcgattc catgccttcg
    13561 atttgtccgc gccgcttgtt cagatcggaa atcacatccc ccatactttc ctctggagtt
    13621 actacttcca ccttcatgat cggctctttt aaaacaggac cggctttttc gctggcactt
    13681 ctgaatgcct gaatagcaca tatttcgaat gacaactgat cggagtctac cggatggtaa
    13741 gaaccatcaa ttaccgtaac tttcagcttg tccaacgcat aaccggcgag aacaccgttc
    13801 ttcatggcgc gtgcaaaacc cttctgaatg gacggaatat attccttggg gatatttcca
    13861 ccttttacct catcgataaa ctgcaattcc ccttcgaaat cggcatcggc cggttctacc
    13921 cgtacgataa tgtcggcaaa tttaccgcga cctccagtct gcttcttgta aacctcacgc
    13981 agctcgacag gtttggtaat agcttcttta taagatacct gaggacgtcc ctgattagct
    14041 tcgaccttaa actcacgttt caaccggtcg ataataatct ccaggtgaag ttctcccatt
    14101 ccggagataa ccgtctggcc ggtttcttca tccgtcttaa ccgtaaatgt cgggtcttct
    14161 tccgccagtt tagccaaacc aacggagagt ttatccaaat ctttctgggt tttaggctca
    14221 acagcaatac cgataaccgg atcggggaaa tccatcgatt ccaggacaat gggtttgctt
    14281 tcatcacaaa gcgtgtcgcc cgtgcgaata tccttaaatc caaccccggc tcctatgtca
    14341 ccgcatccga taaattcctt cggattctgt ttgttggaat gcatctggaa cagacgtgag
    14401 atacgttctt tttttcccga acggggattg taaacgtaag aaccggcttc cagttctccc
    14461 gaataaacac ggaagaagca gagacgtccc acatatgggt cagtcgcgat tttgaacgcc
    14521 agggcgcaca tcggctcgtt aggatcagga cgtctgacga ctattttgtc tgtatctctc
    14581 ggatcaacgc cctcaatagc atcggtgtcg accgggctgg gcaagtaaga acaaacagca
    14641 tccagcaacg tttgtactcc tttgtttttg aatgaagagc cacagatcat cggatttatc
    14701 tgcatggaaa gtgttccctt ccttacagct gctctcacct catcctccgt gatggttgaa
    14761 ggatcgtcga aaaacttctc catcaacaca tcatcgaact cagcaaccgt ttcgagcatc
    14821 ttttctctcc actcgttggc ttcatccaat aaatcaacag gtatctcctc tatgtgatac
    14881 tcagcaccca tggtctcatc gtgccaatac aacgctttca ttgtaatcaa atccaccacg
    14941 cccttgaagt tctcttctgc gcctatgggg atttggatgg gacaaggagt tgcaccaagt
    15001 acttctttta cctgacgaac tacctcaaaa aagttagccc cagaacggtc catcttattc
    15061 acgtaaccaa ttcgcggcac tttgtattta tctgcctgac gccacacagt ttccgattgc
    15121 ggctcaaccc cacctacggc acaaaatgcc gccacggctc cgtcgagaat acgcaatgaa
    15181 cgctcaactt ctacggtgaa gtcaacgtgt cccggagtat caatcagatt aattttgtat
    15241 gttttgtcat tatatttcca gttcgcggtt gtggccgcag aagaaatggt tataccccgc
    15301 tcctgctcct gctccatcca gtccatcgtg gccgctccat cgtgtacctc tccaatttta
    15361 tgggttaatc ccgtgtaaaa caagatacgt tccgacgtag tcgtcttgcc ggcatcaatg
    15421 tgcgccatga tacctatatt gcgagtgaaa attaatgctt cttttgaacc ttttgccatc
    15481 ttttttacct aacagtctgt ttatttaaaa tctgaaatgt gcaaaagccc tgttcgcttc
    15541 tgccattcta tgcatatctt ctttacgttt atatgatcca ccctgattgt tatacgcatc
    15601 aacgatctca gcagccagtt tgtctgccat cgtcttacct cctcttttgc gagcataaag
    15661 aatgaggttt ttcattgaaa tggattcttt tctttcggga cgaatttctg taggcacctg
    15721 aaatgttgct ccgccaacac ggcgtgattt cacttcaacc tgcggtgtta tattgtctaa
    15781 tgcggctttc caaatctcga gagccggttt ttcttcgttg ggcaatttag ccttcactgt
    15841 gtccaatgca gaatagaaaa tatcgtaaga tatactcttc tttccgtcat acataaggtg
    15901 gttaacaaac tttgtcaccc ttacatcacc atatacagga tcgggtagaa cctgtctttt
    15961 tttaggtttt gtttttctca ttgttttaat tgatttgtgt tgtgtttggt tgccgttttt
    16021 tatctgtctt caacaattcc tccgaaccgt ttactcaacc tatagtagct tttcccaaaa
    16081 attccaacag cgattttaca tgaattttaa cttataaaaa ataaagcgta cttttattgt
    16141 gcgggattat ttcttctttg cacccggctt ggcaactgct cccggtttag gacgcttagc
    16201 tccatattta gaacgacgtt gagtacggcc atttaccccg gcagtatcca atgttccgcg
    16261 aacaatgtga taacgtacac ccggaaggtc tttaacacga ccgccacgca ccaatacaat
    16321 agagtgctcc tgcaagttgt gcccttcgcc cggaatataa gcgttcacct ccttagtgtt
    16381 agttaaacgt acacgagcca ctttcctcat tgctgagttt ggttttttgg gagtagtagt
    16441 ataaaccctc acgcagacac cgcgtctttg cggacacgaa tcaagcgcac gggatttact
    16501 tttctcttct ataaccgtac ggccttttct tactaattgt tgaattgtag gcatttttct
    16561 actttctttt tgtcttttat tttactgata aatttaattt ctttacacaa cctcacccaa
    16621 ttataactta aatataatca aatgattaac gtcaaaaacc accgtctctg gggttaaaaa
    16681 cggcacaaaa ttacaaataa tcaacatgat atgcaaatta ttgacggtgt tttttcaact
    16741 attttacaaa atccagacaa ctgatatttt gaaaaaacaa caactatttc ttacgtcgcc
    16801 accgattttt ataaaaaaac aactattttc gcaattcaat gtaacttttt caacatttat
    16861 ccgtcatcca actaatgagt cacgataaat tccatcggat cattttaccg ctaaaggata
    16921 aactcttccg gcttgcgtgg agcatcgtga gagattccgc cgaagccgaa gacattgtac
    16981 aagacgtgtt tttgaagctt tggtcgaaaa ataacgagtg ggaaaacatc gagaatctgg
    17041 aagcctattg tttccgcgct acaaaaaacc tggcgctcga cagagtggag tctctatcga
    17101 tacgaagaac agaaaatatt gctcctgaat tggaaaacgg aatttttacc gatcacgtaa
    17161 cgccgttttt caaactggtg gaaaccgaaa ggaacacatt aatatatagg tgcattaatc
    17221 aactgtcgga aagccagaaa gcggtatttc aactgcgtga gattgaagga atgagttaca
    17281 aggaaatagc gcaagtattg gatattagcg aagatttggt aaaagtgaac ctctttaggg
    17341 ctagaaataa attgaaagaa ttattatcgg gtaaacaaga atgaacaacg aaagaataca
    17401 tacattgctg gagaattact ggaactgcga aacatcggtt tcggaggaaa gggaactaca
    17461 ggaatttttc aacttcggtg acgtccccga tgacttgctg ccgtatacac ctttgtttgc
    17521 ctataaacaa aaccatcagg caatgaaatt gagctgtgat tttgagaaga aacttgatac
    17581 actgatccgc agaaccgacc ggcaaaaaca atacatcacc ataaaaatat tcgagccttt
    17641 cctgcgtatc gccgcatcgg ttattctcat tgccgggatt ggggtatccg tttactttgt
    17701 tgcccgccaa aacaaccggg tctttgccga aacatacaac gatcctcgtg cagccataca
    17761 acaggccacc tcggccttag agaagctatc caaagcatta cgggccaccg aagaagcatc
    17821 tttgaagtcg ctggagcaat tggatgattt agacctggat tggtcggaaa tcgattcgtt
    17881 gagcaccagg attccacttt atgaccccga tttaagtaag aatatcaaac ccgaaaatct
    17941 atgagaaaaa tttttctggt cttgtttacg ttgtttttgg caacttcaac ctttgcaaac
    18001 gatttcatta cgagattcct gaaggagtgc gtcgaaacgg aacggccggt aagtaacgta
    18061 aacataggga aagcgatgct taacaaaatg gcgtcgaata ccagcgatga agaactaaag
    18121 acaacttttc gcgagttaaa cagcattcgc atcattacaa cagaaaacaa agcagactca
    18181 aaattctatt ttgaaaaagc aaaagagatg gcatccagcg aattcacaga ctacaaggag
    18241 gtagtatcgg tgaatgaaag gaggtcgaaa ataaatatcc tgatgaaaaa aaccgacgag
    18301 aaaacgcagg atcttatcct tatggcattg gacgacgaca gtaaattaac gatcataacc
    18361 gtttcgggaa atatagactt caactcaatc tcgaaaattt ccgactcatt gcggaaagga
    18421 gaagcagaag aacctgtttt agagaaagga aacgaaaaaa acaactgacc cggtcagtca
    18481 aaccgaacga gcacaaataa cttttttctt ttttatattt aaacgatgag caggcggcag
    18541 aacgtgagtt tagccgcctg ctgcttttat ggcacaaaaa tacgtttttt ttgaattaaa
    18601 atgcaagaga atttgtaaat ttgcgaaact tttgatacag taggttatta ttactgctca
    18661 catcaaatcc acacctaaac aatatgtata acaaatcact ccatcttgtt ctcgaagacg
    18721 gaacagtatt tcagggaaaa tcatttggtt acgaagcacc ggttgccggt gaagttgttt
    18781 tcagcactgg aatggtcggc tacaccgaaa gcctttcaga tccctcctac ctcgggcaaa
    18841 tcctgacgct gacctacccg cttatcggaa actacggcgt ccccaaagac gaatcgcacc
    18901 aaggcatttc taccttttac gaatcggaaa gaatacaggc aagcggcctt attgtttccg
    18961 atttttcttt tgaatacagc cactggaacg ctgcaaaaag tttaagcgac tggctgaagg
    19021 aaaacaaagt acctgccgtc tacggcatcg acacgcgcga actgaccaag ctggtccggg
    19081 aaaaagggac aatgcttggt aaactggtct ttccgggtga acccgatata ccgtttgtga
    19141 atcccgatga caaaaatcag gttgcaaaag caagctgcaa ggaggtgatt gtctacggca
    19201 gcggaaagca caaagtgata ctggtcgatt gcggtgtgaa aaacaacatc atccgctgcc
    19261 tgctgaaacg ggatacgaca atcgtgcgcg taccctggga ttacgatttt aacgagatgg
    19321 agttcgacgg actgtttatt tccaacggtc cgg
//
LOCUS       DAOSDR010000005        18857 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrimonas sp. isolate AMR_MDS_1826
            NODE_1209_length_18857_cov_4.370705, whole genome shotgun sequence.
ACCESSION   DAOSDR010000005 DAOSDR010000000
VERSION     DAOSDR010000005.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863355
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrimonas sp. (wastewater metagenome)
  ORGANISM  Petrimonas sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Dysgonomonadaceae; Petrimonas.
REFERENCE   1  (bases 1 to 18857)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18857)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:10:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,720
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 35
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18857
                     /organism="Petrimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1209_length_18857_cov_4.370705"
                     /isolate="AMR_MDS_1826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2023866"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2822)
                     /gene="nifJ"
                     /locus_tag="PLI30_00465"
     CDS             complement(<1..2822)
                     /gene="nifJ"
                     /locus_tag="PLI30_00465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006842436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HOI78058.1"
                     /translation="MTKKKNYLTCDGNQAAAHIAYMFSEVAAIYPITPSSTMAEYIDE
                     WAAAGRKNIFGQPVLVQEMQSEAGAAGAVHGSLQAGALTSTFTASQGLLLMLPNMYKI
                     AGELLPGVFHVSARAIAAQALSIFGDHQDVMAARPTGFASFATGSVQEVMDLAAVAHL
                     AAIESRVPFLHFFDGFRTSHEIQKIEALTNDDLAPLINQEALAGFRQRALSPDAPVTR
                     GTAQNDDIYFQSREAANPFYNAVPGIVEKYLEKLAAVTGRKYGLFDYYGDPEAERVII
                     AMGSVTEAIREVVDHKNANGEKVGMVAVHLYRPFKAEAFLSVIPKTARRIAVLDRTKE
                     PGATGQPLYLDVKDSFYGKENAPVIVGGIYGLSSKDTTPGQIISVYDNLAMNMPKNDF
                     TIGIVDDVTFKSLPKVEEVSMDETTYEAKFYGLGSDGTVGANKNSIKIIGDNTDKYVQ
                     AYFAYDSKKSGGFTCSHLRFGDNRIRSTYLVNTPNFVACHVPAYLHLYDVTAGLKKGG
                     TFLLNSLWSKEEVGNHLPDNVKKYLAKNNIKLYIINATNIADEIGLGNRTNTILQSAF
                     FKISNVIPYELAVEQMKKFIVKSYGRKGEEIIKMNYAAVDRGGEVEEVEVLREWADLN
                     VDMVQKDDAPEFIQKVVRPVNAQRGYDLPVSVFVGREDGTWEHGTATYEKRGVAASVP
                     VWNPDNCIQCNQCAYVCPHATIRPFVLDEKEQKGLGEEVALLKTQGKQFEGTAFRIQV
                     DVLDCLGCGNCVDVCPGKKGQSALEMVPITTQYDNQKNWDYMVQHVSSKAHLVDTKLN
                     VKNSQFAKPLFEFSGACSGCGETPYIKLITQLYGDRMMIANATGCSSIYGASAPSTPY
                     TKNEEGKGPSWANSLFEDNAEFGFGFVIAQASMRNRVGDLMQKARKSADFSDSQKELF
                     VQWIENKDNGEAVKEISAQIVGVLTG"
     gene            3161..5329
                     /locus_tag="PLI30_00470"
     CDS             3161..5329
                     /locus_tag="PLI30_00470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019538538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional alpha,alpha-trehalose-phosphate
                     synthase (UDP-forming)/trehalose-phosphatase"
                     /protein_id="HOI78059.1"
                     /translation="MKILIIANRLPVKIERTEDSFVVKRSEGGLATGLGSLETDAEMH
                     WVGWPGVHVDDENEKQEITEKLNELNYHPVFLSGEQIENYYEGYSNSTIWPLCHYFFA
                     YIQYKAEYWEAYRQVNTLFCEEALPFIEDDDIVWIQDYQLMLLPKKIRDKKPKVNIGY
                     FHHIPFPSYELFRVLPEREQVLEGLLGADLIGFHTHDYMRHFISAIYRVLDLNCNLDE
                     ISLKDRIVHVDAFPMGINYEQYHRAPSLPEVKKISKRLIEELGNQTIVLSVDRLDYSK
                     GILHRLNGFANFLENHPEYHKKVSLAMIVVPSRDAVDRYADLKTRIDQYIGKINGMYS
                     TLGWTPVYYFYQSFPFNELVALYDIADIAMVTPLRDGMNLVAKEYLATKRGKPGVLIL
                     SEMAGAAIELTDAIIINPNDTQEIEAAILQALTMPKKEQRIRLNNMQKRISTQTVKKW
                     ANDFVKELLYISKQNNEIFQKIVGKRQLSQIKKEYDQAYTRLILLDYDGTLSPFVKNP
                     EDAVPSKELLNLLKKMTADKKNKVVINSGRNRQVLDKWFKGIDLDFAAEHGAFFKENH
                     KWHKNVQEKITWDDEILRIIEHTIDKTPRSRMEIKDSSLVWHYRNVDVWLAELRQKQL
                     INALMGPASRLNLQIVPGNKIVEIKSPDFNKGSEVKRLLGKNNYDFILAIGDDTTDED
                     MFHALSPGGVSIKVGHFSPAAKYRIPVQSSVIPFFENLIK"
     gene            5354..6442
                     /locus_tag="PLI30_00475"
     CDS             5354..6442
                     /locus_tag="PLI30_00475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019538539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel"
                     /protein_id="HOI78060.1"
                     /translation="MNDITDLITENKWRHIFVSLIFIILFILLTLASNFIFKKLRNRA
                     LRSQSLIDDFIVRLFKVPSAWIIFSILLNIFSSLLDKNTRVYDIIQKTGQVLLILSIG
                     WLIVQAVRAVFRYFQNKLDVNQSDNLTARRRLTQLNMIEKVLIVTVVIVFTGIALMTF
                     ETVKGLGMSLLASAGVLGIVVGLAAQRSVGQILSGVQIAITQPIRLDDVVVIQGEWGR
                     IEEINITYAVVKIWDERRLIVPIDYFLNNPVQNWTRTSSNILGTVFLYVSYGLPVAPL
                     RKKLAGLVKNDPNWDGRVQNIQVTDSKQWYKEIRVLVSSEDSSKNWDLRVSVREKLID
                     FINENYPGSFARISTTGEEKQRHTATDG"
     gene            complement(6429..8222)
                     /locus_tag="PLI30_00480"
     CDS             complement(6429..8222)
                     /locus_tag="PLI30_00480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004299007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 15 protein"
                     /protein_id="HOI78061.1"
                     /translation="MKNLDYGVIGNCRTAALVSKEGSIDWLCLPDFDSPSVFTRLLDE
                     EKGGSFAFLVSGEYVVTQTYLGNTNILSTRFEAPEGAFTVLDYMPRYRTSEKSHYLPP
                     EVHRYLRVLHGKPKMRILYDPKMNYAGEEAVHTVFPNYIRTSSAVDAEDKIYLYSSVN
                     FDVILSGEEFTLTDHQFFLLSYNQKLVAIDENRVLLDYERTKVYWLNWINRSREYRQY
                     GDMVNRSMLVLKLMSYQHSGAMLAAVTTSLPESIGETRNWDYRFCWLRDASMSIDTLL
                     HMKHQGTAERFIGFIKRILKSREDTFQIMYGIRGERELTEEILSHLAGYENSYPVRIG
                     NAAYNQLQNDSLGYLMDVIYKYYLYFPGTLDEVEEIWEVVRNMVRMVLKSWRSPDQSI
                     WEFRTRELNFVFSKVMSWVALDRASLIAELLHKDNYAELWRKEANGIKEEIHEKGWNE
                     ELQCFTQAYENTDHDSSLLLMQFYDFIDARDERYVKTVKAIKENLFHNGLMYRYKAED
                     DFGKPTSSFTICTFWLIEALFVIGETDQAREIFESMITYSNHVGLYSEDLDFETKRQL
                     GNFPQAYSHLAFINTATLLSEEKKLSRFIRP"
     gene            complement(8261..9301)
                     /locus_tag="PLI30_00485"
     CDS             complement(8261..9301)
                     /locus_tag="PLI30_00485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013547620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HOI78062.1"
                     /translation="MIDKNARVLLIYTGGTIGMVENLETGALEAFNFSHLKSNVPEMK
                     RLNFNVDTYLFDPPIDSSEVSPQNWQDMATVVEDNYNDYDGFVILHGTDTMSYTASVL
                     SLMFENLTKPVIFTGSQLPIGKLRTDGKENLITALEIAADKDKSGRPVVPELCIYMQN
                     LLMRGNRTTKVNSENFSAFNSPNYPYLAEVGVDIRYHDKFILQPDYDKPVIFNFRLDH
                     NVAILKLFPGIQESVVRSILSVPALRGVVLETYGSGNAPTDQWFIDALQEAIDQGIVI
                     VNVTQCLYGNVEMHRYETGQHLEKLGVVSGHDITTEAALAKLMILQGNGKTPDQVRRL
                     MSVSLRGELTER"
     gene            9462..9881
                     /locus_tag="PLI30_00490"
     CDS             9462..9881
                     /locus_tag="PLI30_00490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HOI78063.1"
                     /translation="MTHSIKTIWKDNNIFETDVDGHNVVIDLAEDAGGNDAGPRPKKL
                     LLVAAAGCTGLDVVEILKKMRIDIKGFNVQIESGMTDEYPKQYTDLKVVYEFEGKDLP
                     KDKLERACQLSFDKYCGVLAMYKKAVPVSYEVKITDV"
     gene            9992..11251
                     /locus_tag="PLI30_00495"
     CDS             9992..11251
                     /locus_tag="PLI30_00495"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HOI78064.1"
                     /translation="MQTIDTFNFAGKKAFIRVDFNVPLDENFKITDDTRIRAAIPTLK
                     KILKDGGSVIIGSHLGRPKGPTEKYSLKHILPRVSELLGVDVHFANDCIGEETEAKAA
                     ALRPGEALLLENLRFYAEEEGKPRLPEGATEEETAAAKKAVKESQKEFTKNLASYADV
                     YVNDAFGTAHRAHASTALIADYFDAGNKMFGYLMQNEVNAVEKVMSDTERPFTAIMGG
                     SKVSTKIELIKNLLDKVDNLILAGGMTYTFAKAGGGKIGDSIVENDKLDLANEIVDLA
                     KEKGVNLVLATDAKLADSFSNDAKTDFGSVKEIPDGWEGLDIGPETEKVFAEVIKNSK
                     TILWNGPVGVFEFDNFDKGSRAVADAIVEATQKGAFSLIGGGDSVACINKFGLADDVS
                     YVSTGGGALLEMIEGKVLPGIKAIRGY"
     gene            11457..12626
                     /locus_tag="PLI30_00500"
     CDS             11457..12626
                     /locus_tag="PLI30_00500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010538790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF418 domain-containing protein"
                     /protein_id="HOI78065.1"
                     /translation="MEISTGKTSRIEVVDSLRGFAIMAILLVHNVDHFFFPEFPTHSP
                     HWLTILDQLSVKIIFTLFSGKTYAIFTMLFGFTFYIQHNNQKKKGKDFGYRFLWRMAL
                     LAVFAVLNAAFFPAGDVLMLFVYVSLVLFICRNWPDRAVLVFIIVLLLQPVEWIHFIL
                     SRFNPAYQLPDFGAVSLHQQVVENTKNGSFLDFIRVNVTLGQKVSLMWALGSGRFLQA
                     AGLFLLGFYIGRKQFFINTPDNLRFWKRVLIISSVSFILFNIIKKLLLQSDPMAQQTV
                     GLIFDIWYKLALTFVLIASFVLLYEKRFFRKRLSGLRFYGKMSLTNYVSQSIFGALIY
                     FPIGLYLAPYCGRTFSLVIGIAVFSLQMMFSRFWLGKHKQGPLEGIWHKWTWINS"
     gene            12845..15307
                     /gene="pheT"
                     /locus_tag="PLI30_00505"
     CDS             12845..15307
                     /gene="pheT"
                     /locus_tag="PLI30_00505"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005937423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HOI78066.1"
                     /translation="MNISYNWLKDYLQFDLSPQETAAALTSIGLETGSVEEIQTVKGG
                     LEGLVIGEVLTCTHHPNSDHLHLTTVNIGNGEEPLKIVCGAPNVAAGQKVVVATVGTK
                     IYFGDEPVTIKRSKIRGEESFGMICAEDEIGIGTSHEGIIVLPDDAEVGMLAKDYYNV
                     KSDFVLEVDITPNRIDAASHYGVARDLAAYLKQSGKPFSLTKPSVDGFKTDKSEGGID
                     VVVENSAACPRYSGLTVRGVTVKESPDWLKNKLLVIGLRPINNIVDITNFILHETGHP
                     MHAFDVNYIEGNTVVVRTLPRKSKFLTLDEQERELNENDLMICNTKEGMCIAGVFGGL
                     KSGVTESTTDVFLESAYFNPTWVRKTARRHGLNTDSSFRFERGADPGNTVYVLKRAAM
                     LIKEIAGGEIEGEIRDIYPVKINNAQVVLPFRKVNSLIGKEIPKETIKNILQSLDIET
                     ENESEEALTLSIPTYRVDVTRDVDVIEEILRIYGYNNVDFSDSLRANLSYQTPTDRKH
                     KLQTLISEQLTASGFSEIMNNSLTRKSYYENNTTFPGENGVKLLNPLSNDLGVMRQTL
                     LYGGLESIAYNRNRKHYDLHFYEFGNCYFFDTDRKQEGNILSGYSEETHLGLWICGDS
                     NPKNWAAPQTETSVFQLKAHIENILNRLGFTQNQVTFEPTQSGIYASGMNIRTFNRQI
                     GSFGIISGNILKKFDIDTDVFFAEMSWDALLKETSASKVHFSEISRFPSVSRDLALLV
                     DKSVTFAAIEQTAKKAERKLLQELSLFDVYEGKNLPEGKKSYAVNFVLQDKEKTLNDK
                     QIDAVMQKIQQNLEKELGALLR"
     gene            15338..16048
                     /locus_tag="PLI30_00510"
     CDS             15338..16048
                     /locus_tag="PLI30_00510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005856672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HOI78067.1"
                     /translation="MGRAFEYRKATKLKRWGNMARVFTKLGKQITIATRDGGPDPENN
                     PRLRVLIQQAKKENMPKENVERAIKKATDKDVSDYKEMVYEGYGPYGVAIVVETATDN
                     PTRTVANVRSYFNKHGGSLGTSGSLEFLFDHKCVFKISPKEGVSLEDLELELIDYGVD
                     EVEEDEGDIILYGEFKSYSDIQSYLEENGFEIHSAEFERIPHDTKSLSDEQRTQIEKL
                     LEKFEEDDDVQNVFHNME"
     gene            complement(16104..17204)
                     /gene="meaB"
                     /locus_tag="PLI30_00515"
     CDS             complement(16104..17204)
                     /gene="meaB"
                     /locus_tag="PLI30_00515"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005824008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HOI78068.1"
                     /translation="MHHPENDPKYLGLNVNKGVVQPPSINPYLHLRKKQQRKEYSVKE
                     FAEGILAGNITVLSQAVTLVESSKPEHQAMAQAIIEKCLPYSGNAIRVGITGVPGAGK
                     STSIDAFGMYLVEKGHKLAVLAIDPSSERTKGSILGDKTRMERLSVQENAFIRPSPSA
                     GSLGGVARKTRETIVLCEAAGFDYIFVETVGVGQSETAVHSMVDFFLLIQLAGTGDEL
                     QGIKRGIMEMADGIVINKADGDNVEKAKMAQSQLRNALHLFPLPESEWSPEVLTYSGY
                     YALGIEEVWDMIHRYIEFVKDSGYFDVRRNKQSKFWMYETINERLRNDFYQNAEIEQL
                     MPLLESEVLSARKSSFVAAKEALDRYYSESKE"
     gene            complement(17217..17606)
                     /locus_tag="PLI30_00520"
     CDS             complement(17217..17606)
                     /locus_tag="PLI30_00520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006842670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1573 domain-containing protein"
                     /protein_id="HOI78069.1"
                     /translation="MKKISVILFFSIVLSGFAFSQSGPKMQFKKSVHDFGTIKEEIGA
                     VSTQFEFTNMGKSPLIIQRVSASCGCTTPSYTKEPILPGKSGKIDAKYSTTARPGTFN
                     KTITVYTNVPDTVYVLSIKGNVTPRKR"
     gene            complement(17703..>18857)
                     /locus_tag="PLI30_00525"
     CDS             complement(17703..>18857)
                     /locus_tag="PLI30_00525"
                     /inference="COORDINATES: protein motif:HMM:NF036531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI78070.1"
                     /translation="RFEQLKKQNKDWQKLKTIPVSDLEYLYVRSAYNQYPLDSKTKEM
                     ADFYTSIVEKNWTQFGLYERSLIVVLMQKDGKQNVVRDMLRSYREHAVLSEEMGMFWA
                     NNRAHVFMSQSAVLVHTFIMDAFRVGGAKADELDHMKRWLLKQKQTQMWESTHATIDA
                     VYALLSAGTDWFSADNNTTVTVGGKVIAPQQQAQGTGYFKETWHKSEITPQMGKVKIE
                     NSGNAPAWGALYWQYYEDLDKITKTGGSLNVEKMLFVEETDVSGRKLVPITENRMLRV
                     GDKVIVRLTLRTDRDLEFVHLKDMRAASFEPLEQLSGMKWQEGVIYFQASKDASTNFY
                     FDVLPRGTYVLEYGAFVTRTGDYSNGITTVQCMYAPEFSSHTAGMKVNVR"
BASE COUNT         5316 a         4452 c         4117 g         4972 t
ORIGIN      
        1 ataccggtaa gcacgcccac aatctgagcc gaaatctctt ttacggcttc tccgttgtcc
       61 ttgttttcga tccactggac aaacagctcc ttttgagaat cagaaaagtc agccgattta
      121 cgggctttct gcatcaaatc ccctacgcgg ttacgcatgc ttgcctgagc aattacaaat
      181 ccaaaaccaa actcggcatt atcctcgaac aacgagttag cccacgacgg ccctttgcct
      241 tcttcgtttt tggtatatgg ggttgacgga gccgacgcac cgtaaatgga agaacatccg
      301 gtagcattgg caatcatcat acggtcgcca tacagttgcg taataagctt gatgtacgga
      361 gtctcaccgc aacccgagca ggcaccggag aactcaaaca acggttttgc gaactgggag
      421 ttttttacgt tcagcttggt atccaccaga tgagctttac tgctgacgtg ttgaaccata
      481 taatcccagt tcttctggtt atcgtattgt gtggtaatgg gaaccatttc gagagcgctc
      541 tgccctttct tgcccggaca aacatccacg caatttccac aacccagaca atccagcaca
      601 tccacttgaa tccggaaagc ggttccctcg aactgttttc cctgtgtttt caatagtgcc
      661 acctcttctc ccagcccttt ctgttctttc tcatcgagca cgaacggacg gatagtagcg
      721 tgcggacaaa cgtaggcaca ctggttacac tggatgcagt tgtcgggatt ccaaacagga
      781 accgaagcag ccacaccgcg tttttcgtaa gtagccgtac cgtgttccca tgttccgtct
      841 tcacggccca caaaaaccga aaccggcaaa tcatatccgc gctgggcatt tacgggacga
      901 accacttttt gaataaattc cggagcatcg tctttttgta ccatatccac attcaggtcc
      961 gcccattccc gaagaacttc aacctcttca acttctcctc cgcggtcaac agccgcatag
     1021 ttcattttta taatctcttc cccttttctg ccgtacgatt tcacaatgaa tttcttcatc
     1081 tgttccaccg ccagctcata agggattacg ttggatattt taaaaaatgc cgactggaga
     1141 atggtattgg tcctgttgcc caatcctatc tcatccgcta tgtttgtagc atttatgatg
     1201 tacaacttaa tgttgttttt ggccaagtat ttcttcacgt tgtccggcaa atggttaccg
     1261 acttcttctt tcgaccacaa tgaattgagc aggaaggttc ctcccttttt cagtccagcg
     1321 gtaacatcgt aaagatgcag gtatgctgga acgtgacagg ctacgaagtt gggtgtattc
     1381 accagataag tggagcggat acggttatct ccaaaacgga gatgggaaca ggtaaatcct
     1441 cccgatttct tggagtcgta agcaaaatag gcctgcacat acttatcggt attatccccg
     1501 ataatcttga tggagttttt gttggctcca accgttccgt cggatcccag gccgtaaaac
     1561 ttggcttcgt aggttgtttc atccatcgag acctcctcaa ctttcggcaa cgatttaaat
     1621 gtcacatcat ccacaattcc gatggtgaaa tcgtttttcg gcatgttcat cgccaggttg
     1681 tcgtaaaccg agataatctg tcccggggta gtatctttag aggataaacc gtaaatacct
     1741 ccgacaatca ccggggcatt ctcttttccg tagaaactgt ctttcacgtc cagataaaga
     1801 ggctgtccgg tagcaccggg ttcttttgtc ctatcgagaa cagcaatcct acgggctgtt
     1861 ttcgggatga ccgacagaaa tgcttcggct ttaaaaggac ggtacaggtg aacggctacc
     1921 atacccactt tttcgccgtt ggcgtttttg tggtcaacca cttcacgaat agcttcagta
     1981 acggaaccca tagcaataat cactctttcc gcttcaggat caccgtaata atcaaacaat
     2041 ccgtacttac ggccggttac agctgccagt ttttccaggt atttctcaac aatacccgga
     2101 acagcgttgt aaaaaggatt agccgcttca cgggactgaa aataaatatc atcgttttga
     2161 gccgttcctc gtgtaacagg tgcatcagga ctcaacgctc tctgccggaa accggctaat
     2221 gcctcctgat tgatcaacgg agccagatca tcgtttgtca acgcttcaat cttctgaatt
     2281 tcgtgtgaag tgcggaatcc atcaaaaaaa tgcaaaaacg gtaccctgct ctctattgct
     2341 gccaaatggg caacagctgc caaatccatc acttcctgta cagatcccgt agcgaacgat
     2401 gcaaaaccgg ttgggcgtgc tgccataacg tcctggtggt caccaaaaat agacaatgcc
     2461 tgtgcagcaa tggcccgtgc cgatacatgg aatactccgg ggagcaactc tcctgcaatt
     2521 ttatacatgt tgggcaacat caacaacaaa ccctgtgatg cggtaaatgt ggatgttaat
     2581 gcccccgcct gcaacgaacc gtgaacagcg cctgcggctc cagcttcgga ttgcatctcc
     2641 tgtaccagta caggctgccc gaaaatgttt tttcttcccg cagcggccca ttcatcgatg
     2701 tattctgcca tcgtcgacga aggtgtaatc gggtaaatag cagctacctc actaaacata
     2761 taggcgatat gtgctgcagc ctgattaccg tcacaggtta aatagttctt cttttttgtc
     2821 atgacaatat gattatttta ttgatttgat tttacgaaag tcttgaagaa tataagcatt
     2881 gtgaaatctg agtacgaaat tactataaaa atatggaaca ggcaaaagat tgcatttttt
     2941 ttagattcct gacagaagtt caacaccaat tcctccctta tattataaaa atatgttaaa
     3001 aattgatctt tcctggaacg ggactgttat atttttaaac cgattggaaa aaaattgatt
     3061 gaactgctcg ccaattatcc aggttcaatg ctgtatatca acgcaggaca ccttatatcc
     3121 tgcgggaacc aataacaaaa gtgaataaaa atagaatcgt atgaaaatcc ttattatagc
     3181 caacaggctt cctgtcaaaa ttgaaagaac cgaagactcc tttgtcgtaa aacgcagcga
     3241 aggagggctg gccacaggat tggggtcttt ggaaaccgat gcggaaatgc attgggtggg
     3301 gtggcctgga gtccatgtgg atgatgaaaa tgaaaagcag gagattaccg aaaagctgaa
     3361 tgagttgaat tatcatccgg tttttctgtc cggagaacag attgaaaatt attacgaagg
     3421 ttacagcaac agcaccatat ggccgctatg tcattacttt ttcgcataca tacagtacaa
     3481 agccgagtac tgggaagcgt accggcaggt gaatacactg ttctgtgaag aagcgctgcc
     3541 ctttatagaa gacgatgata ttgtctggat acaagattat caactgatgt tgctgccgaa
     3601 aaaaatccgc gacaaaaagc ccaaagtaaa catcgggtat ttccaccaca taccctttcc
     3661 gtcttacgaa ctattccgtg tgctcccgga aagggaacaa gtactggaag ggttgcttgg
     3721 agccgattta atcggatttc acacgcacga ctacatgcgt cattttatca gtgccatcta
     3781 ccgggttctc gacctgaact gcaacctcga cgagatcagc ctgaaagacc gtatcgtaca
     3841 tgtggatgca ttccctatgg gtataaatta tgaacagtat catcgggcgc cctcactccc
     3901 ggaggtaaag aaaatatcaa aaaggctgat agaagaactg ggtaatcaaa ccattgtttt
     3961 gtcggtcgac cggctcgact acagcaaggg aattctgcac cggttgaacg ggttcgccaa
     4021 ttttcttgaa aatcatcccg aataccacaa aaaggtatcg ctggcaatga tagtcgtacc
     4081 ttcccgcgat gcagtggaca ggtatgccga cctgaaaacc cggatagacc aatatattgg
     4141 taaaataaac ggcatgtatt ctaccctcgg ctggacccct gtgtactatt tttaccagag
     4201 tttccccttc aatgaactgg tagcacttta cgacattgca gacatcgcta tggttacccc
     4261 gctacgcgac ggaatgaacc tggtagccaa agaatatctt gccaccaaac ggggaaagcc
     4321 cggcgtattg atactcagcg agatggcggg ggcagccatt gaactgaccg atgccatcat
     4381 tatcaatccg aacgatacac aggaaattga agcggctatt ctccaggcat tgaccatgcc
     4441 gaaaaaggaa caacgtatcc ggctcaacaa catgcaaaaa cggatatcca cccaaaccgt
     4501 aaaaaaatgg gcgaatgatt tcgtgaagga acttctctac ataagtaagc aaaacaacga
     4561 aatttttcag aaaatcgtag gtaaacgaca gctctcacaa ataaaaaagg agtacgacca
     4621 ggcttacaca agattaatct tgctcgatta cgacgggacc ttgtcgccct tcgtgaaaaa
     4681 ccccgaagat gcagtccctt ccaaagaatt gttgaactta ttaaaaaaga tgaccgccga
     4741 caaaaagaac aaagtagtca tcaacagcgg aaggaatcgt caggtgctgg acaagtggtt
     4801 caaggggatc gatttggact ttgccgctga gcacggcgca tttttcaagg aaaaccacaa
     4861 atggcataaa aatgttcagg aaaaaataac gtgggacgat gagatccttc gaatcataga
     4921 gcacaccatc gacaaaactc cccgctcacg tatggaaata aaggattcct cgctggtctg
     4981 gcattacagg aacgtggatg tatggctagc cgaactacgc cagaagcagc ttataaatgc
     5041 actgatgggg ccggcttcac gcctgaactt gcagatcgtt cccggaaata aaatcgtgga
     5101 aatcaaatct ccggacttta acaaaggcag cgaagtgaaa cgactgttgg ggaaaaataa
     5161 ttacgacttt atccttgcca tcggcgacga caccaccgac gaagatatgt tccacgcact
     5221 ttcgcccggc ggggtgagca taaaagtagg gcatttttcg cctgctgcta aataccggat
     5281 tccggttcaa tcctcggtaa ttcctttttt tgaaaacctg attaaataag agactttttt
     5341 gactttttat cttatgaacg atataactga cctgataacg gaaaataagt ggcgccacat
     5401 ttttgtttct cttatcttca tcatcctgtt tattttgctc actttggctt ccaacttcat
     5461 ttttaaaaaa ttgcgcaatc gtgcactgag aagccaaagc ctgatagatg attttatcgt
     5521 gcggcttttt aaagtcccgt ctgcgtggat cattttctcc attctactga atattttcag
     5581 ttcattactc gataagaata cccgggttta cgatatcata cagaaaacag gacaggtatt
     5641 gctgattctt tccatcgggt ggctgatagt ccaggcagtc cgtgccgtat tccggtactt
     5701 ccagaacaaa cttgatgtta atcagtccga taacctgacc gcacgcaggc ggctcaccca
     5761 gctgaatatg attgagaagg tactcatcgt aaccgtagtg atcgttttca ccggaattgc
     5821 attaatgact ttcgaaaccg taaagggatt gggcatgagc ttactggcct cagccggtgt
     5881 actgggaatt gtggtcggac tggcagcaca acggagtgtc ggacaaattt tgtcgggtgt
     5941 acaaatagct ataacgcaac ccatccgcct ggacgatgtg gtagtcattc aaggtgaatg
     6001 ggggcgcatc gaggagataa acatcaccta tgcggttgta aaaatctggg acgagagacg
     6061 gctcattgta cccatcgatt atttcctgaa taaccccgta caaaactgga caagaaccag
     6121 ctccaatatt ctggggacag tattcctgta cgtttcttac ggcctgccag tagcaccgct
     6181 tcgtaaaaag cttgccggtt tagtgaagaa cgaccccaac tgggacgggc gcgtacaaaa
     6241 cattcaagtt actgacagca aacagtggta taaggaaatc cgtgtactgg taagcagcga
     6301 agattcctct aaaaactggg atttacgcgt gtcagtccgc gaaaagctta tcgattttat
     6361 caacgagaat tatccggggt cgttcgcaag gatttccaca acgggtgaag aaaaacaacg
     6421 acacactgct acggacggat aaacctcgac agcttcttct cttcagaaag tagcgtggcc
     6481 gtattgatga acgccaggtg agagtatgcc tgcgggaaat tcccgagctg gcgcttggtc
     6541 tcaaaatcca ggtcttcgct gtacaacccc acgtgattgg aataagtgat catcgattcg
     6601 aaaatttcac gagcctgatc cgtctctccg attacaaaaa gcgcttctat cagccaaaac
     6661 gtgcagatgg tgaacgatga ggtgggtttt ccaaaatcgt cttcagcttt atacctgtac
     6721 atcaacccgt tgtgaaacaa gttctctttg attgccttca cggttttcac gtaacgctca
     6781 tcgcgggcgt caataaagtc ataaaactgc atcagcagaa gcgaagagtc gtgatcggta
     6841 ttctcataag cctgcgtaaa gcattgcaat tcttcgttcc agcccttttc gtgtatctcc
     6901 tccttaatac cgtttgcttc ttttcgccac aattccgcgt aattgtcttt gtgcagcagc
     6961 tctgcaatca gagacgccct gtcaagtgcc acccagctca tcactttgga aaacacaaaa
     7021 ttcaattcac gtgtgcggaa ctcccaaatg ctctgatcgg gagagcgcca cgatttcagc
     7081 accatccgca ccatgtttct cacgacttcc cagatctcct ccacttcgtc gagcgtcccc
     7141 ggaaagtaca ggtagtattt gtatattaca tccatcaggt atcccagtga atcgttctgc
     7201 aactggttat aagcagcgtt tccgatacgg acgggataag aattctcata tccggccagg
     7261 tgggataaaa tttcttcggt caattccctc tcaccccgga tcccgtacat tatctgaaaa
     7321 gtatcctcac gcgacttcag aatacgtttt atgaacccta taaatcgttc ggccgtgccc
     7381 tgatgtttca tgtgcagcaa ggtatcgata gacatggaag catcacgaag ccagcagaag
     7441 cgataatccc agttacgggt ttccccgatg ctttcgggca gactggttgt aacggctgcc
     7501 aacatggcac cggaatgctg gtacgacatt agtttcaaca ccagcatact gcggttaacc
     7561 atatctccgt attgacggta ctcgcgtgaa cggttaatcc agttaagcca gtacactttc
     7621 gtacgctcgt aatccagcaa gacccggttt tcgtctatcg ctacaagctt ttggttgtaa
     7681 gaaagcagaa aaaactgatg atccgtaagc gtgaactctt caccgctcag gataacgtcg
     7741 aaattcacgc tcgagtagag gtaaatcttg tcctcggcat ctaccgctga cgatgttctt
     7801 atgtagttgg ggaaaaccgt atgcacagcc tcttctccgg cataattcat tttcgggtca
     7861 tacaaaatgc gcatttttgg ctttccgtgc aaaacacgca ggtacctgtg gacttcgggc
     7921 ggcaagtagt ggcttttttc gcttgtcctg taccggggca tatagtccag cacggtaaat
     7981 gctccctcag gtgcttcaaa ccgtgtggaa aggatattgg tgttacccag atatgtttgc
     8041 gttacaacat actcacccga cactagaaaa gcgaagctcc ctcctttttc ctcatccaac
     8101 agacgggtaa aaaccgaagg agaatcgaaa tcgggcaaac aaagccaatc gatactccct
     8161 tctttggaca ctaatgccgc cgttcggcaa tttcctatca ctccgtaatc taaattcttc
     8221 atttgttttc ttatgtatgt tgaaaacaaa gataagaatt ttacctttcc gtcagctctc
     8281 ctcgtaacga aaccgacatc aacctcctta cctgatcggg ggttttgccg ttgccctgca
     8341 aaatcattaa cttggccaaa gcagcctccg tggtgatgtc gtgccccgaa acaaccccta
     8401 atttttccaa atgctgaccc gtttcatacc ggtgcatttc cacgtttccg taaaggcatt
     8461 gcgtcacgtt aacaataaca attccttggt ctatcgcctc ctgcaacgca tcaataaacc
     8521 actgatcggt aggcgcatta cccgaaccgt aagtttccag cacaacacct ctcagtgcag
     8581 gaaccgaaag gattgacctc accacacttt cctgaatacc ggggaataat ttcaggatag
     8641 caacgttgtg atcgagcctg aaattaaata tgacaggctt atcataatcc ggctgtaaaa
     8701 taaacttatc gtggtaacga atatctaccc ccacctcagc caggtacgga taattaggac
     8761 tgttaaaagc actgaagttc tccgagttta ctttcgttgt tcgatttcca cgcatcagca
     8821 ggttttgcat ataaatacac aattcaggca cgactggcct tcccgattta tctttatcgg
     8881 cagcaatctc aagagcagta atcaggtttt ccttcccatc tgtgcggagt ttaccgatag
     8941 gcaactgtga tccggtgaat ataacgggtt ttgtcaggtt ctcgaacatc aaactcagta
     9001 ccgatgccgt ataagacatc gtatcggtgc cgtgcaaaat cacaaaaccg tcgtaatcgt
     9061 tgtagttatc ttccactacc gtggccatat cctgccagtt ttgaggactg acttccgatg
     9121 aatcaatcgg tggatcaaac aagtaggtgt ctacattgaa atttaaacgc ttcatctcag
     9181 gcacgtttga cttcaaatga ctgaaattaa aggcttccag ggctccggtt tccaggttct
     9241 caaccatccc gatggttcca cccgtgtaaa tgagcaaaac tcgtgcgttt ttgtcaatca
     9301 taatagccta aattttcaca aaaatacaaa aacaattgac actttgatac gaaatactga
     9361 cttttttcaa agaacaaaag tatctttttt ttgtttaatc aaaaagcgga aactgaacgg
     9421 atgacaggca tcggaaaaat caaccatcaa acattacatt tatgacacat tcaattaaaa
     9481 ccatttggaa agataacaac atttttgaaa ccgacgttga cggacacaac gtggtaattg
     9541 acctggcgga agatgccgga ggcaatgatg cagggccacg gcctaaaaaa ctgcttctgg
     9601 tagccgcagc aggttgtaca gggcttgatg tggtagaaat cctgaaaaaa atgcgcatcg
     9661 acattaaagg atttaacgtt caaatagaat ccggcatgac agacgaatat cccaaacaat
     9721 ataccgacct gaaggtagta tacgaattcg agggtaaaga tttaccaaaa gataaattgg
     9781 agcgtgcatg ccaattatcg ttcgataaat attgcggcgt gttggcaatg tataaaaaag
     9841 cggttccggt ttcgtacgaa gtgaaaatca cggatgtgta actttttttg cataattaaa
     9901 gaaaaaattc tgtattttcc ttatctttgt gttccaattg caaagattgt ttatacactc
     9961 caatgaatat attctcataa aataaaaaat tatgcaaaca attgacactt tcaattttgc
    10021 cggtaaaaag gcattcattc gtgtggattt caacgtgccg ctcgatgaaa acttcaagat
    10081 tactgacgac acccgcattc gtgcagctat ccccacgttg aaaaaaatcc tcaaggacgg
    10141 ggggagcgtg atcattggct cacacttggg ccggccgaaa ggtcctaccg aaaaatattc
    10201 gttgaaacat atccttccgc gggtttccga attactgggt gttgatgttc attttgccaa
    10261 cgattgcatt ggtgaagaaa ccgaagcaaa agcagccgct ttacgtccgg gagaagcctt
    10321 attgctcgaa aaccttcgtt tctacgctga ggaagaagga aaaccccggc taccggaagg
    10381 ggctacggaa gaagaaacgg cagcagcaaa aaaagcagta aaagaatcgc aaaaagaatt
    10441 taccaagaat ttggcttcct atgccgatgt ttatgtaaac gatgcctttg gtaccgcaca
    10501 ccgggcacac gcctcaaccg cacttattgc tgattatttc gatgcaggaa acaagatgtt
    10561 cggctatttg atgcagaatg aggttaatgc cgtagaaaaa gtgatgtccg acacggagag
    10621 gccattcaca gccattatgg gtggatcaaa ggtttccacg aaaattgaac ttattaaaaa
    10681 cctgctcgac aaagtagaca acctgatcct tgccggcgga atgacctata catttgcaaa
    10741 agccggcggc ggaaaaatcg gggactctat cgttgaaaat gacaagcttg acctggctaa
    10801 cgaaatcgtt gatctggcta aggagaaagg cgtaaacctg gtactggcaa ccgacgcaaa
    10861 acttgccgac agtttcagca atgatgccaa gacggatttt gggtctgtaa aagaaattcc
    10921 tgacggctgg gaagggctgg acattggtcc cgaaacggaa aaagtattcg ctgaagtgat
    10981 caagaactcc aagaccattt tatggaatgg ccccgtgggc gtatttgaat ttgacaattt
    11041 cgacaaaggc tcacgcgcag tggctgacgc catcgtggaa gctactcaaa aaggagcttt
    11101 ctcccttata ggaggaggcg attcggtagc ctgtatcaat aaattcggac ttgctgacga
    11161 tgtttcgtat gtctccaccg gcggcggagc gctgcttgaa atgatcgaag gcaaggtact
    11221 tccgggaatc aaagcgataa gggggtattg ataaaaatgc aggtgtacac ttcgtgacac
    11281 cttaagatat taaccgtacg gacagctatt gttcgtgcgg ttttttatct tcacctgatt
    11341 accttgcgat aatacgtgag tcggacaagc ggctaaagat ttttttaatc cggatgattt
    11401 ttttttaaag aaatgcttat gtttgcaaat tcattttaaa aatcacaagg ttaattatgg
    11461 aaatctcaac aggcaagaca agtcgtatag aagtagtgga ttccttacgc ggatttgcaa
    11521 taatggcaat cctcctggta cacaatgtcg accatttctt tttcccggaa tttcctaccc
    11581 attcgcctca ctggcttact attctggacc agctctccgt gaaaattatt ttcactcttt
    11641 ttagcggaaa aacgtatgcc atcttcacca tgcttttcgg ctttacgttt tatatccagc
    11701 acaacaatca aaagaaaaaa gggaaagatt tcgggtaccg ttttttgtgg cgaatggccc
    11761 ttcttgccgt ctttgccgtc cttaatgcgg ccttctttcc tgcgggagat gtgctcatgc
    11821 tgttcgtcta cgtttcactc gtcctgttta tttgccgcaa ctggccggac agagccgttc
    11881 tggtattcat cattgtcttg ctgcttcaac cggtagaatg gatacatttt attttatccc
    11941 ggtttaaccc ggcgtaccaa ttgcccgatt ttggcgcggt ttcgctgcac cagcaagttg
    12001 tcgagaacac gaaaaacggc agttttcttg attttatccg tgtcaacgta acgcttggcc
    12061 agaaagtaag ccttatgtgg gccctcggtt cgggccgttt tctgcaagct gccggattgt
    12121 tcctgttggg gttttatatc ggaagaaaac agttcttcat caacactccc gataatttac
    12181 gtttctggaa aagggttttg ataatttcat ccgtttcctt tatcctgttt aacataatca
    12241 aaaaactgct gttgcagtcc gaccccatgg cccaacaaac cgtgggcctt atattcgata
    12301 tctggtataa attagcgctt acttttgttc tgatagcatc gtttgtgttg ctttacgaaa
    12361 agcgtttttt tagaaaaaga ttgtccggct tacgttttta tgggaaaatg agccttacca
    12421 attatgtctc ccagtccatc ttcggggcac tgatctattt ccccatcgga ctgtacctgg
    12481 ctccgtattg cgggcgtaca ttcagcctgg tgatagggat agctgttttt tccctgcaga
    12541 tgatgttttc caggttctgg ctggggaaac acaagcaagg ccctctggaa gggatttggc
    12601 acaaatggac atggataaat agttaaccac ttcccatccg cattaaaagg gcatgtatgc
    12661 cccgtaaatc aggatttata cttgaggatt attcttcagg atctacaaag tttcagaact
    12721 ttgacggagg ctatcgtatt tttttgtaat tttgaacact ttttcttgaa gcaaaaccca
    12781 tattagctat tctgagtaaa aacgaagaat ctgagcctgg gttgtcaaaa taaacgactt
    12841 tagaatgaac atctcttaca attggctgaa agattacctg caattcgatc tttcaccaca
    12901 agaaacagca gcagcactca cctccattgg attggaaaca ggtagtgtgg aggaaattca
    12961 aaccgtcaag ggtggtcttg aaggattagt tatcggcgaa gtgctgacct gcacccatca
    13021 tcccaactcc gaccacctgc acctgacaac tgtaaacatc ggaaatggag aagaaccgtt
    13081 aaaaatagtc tgcggagccc ctaacgtggc tgccggacaa aaagtggtgg tagctacagt
    13141 cggcactaaa atctattttg gtgatgaacc agtaaccatt aaacgctcca aaatccgggg
    13201 agaagaatct tttggaatga tctgtgcgga agatgaaata ggcattggaa catcacacga
    13261 gggaattatc gttcttcccg acgatgctga ggttggcatg cttgccaagg attattacaa
    13321 cgtaaaaagc gatttcgtcc tcgaagtcga catcaccccc aaccgcatag atgccgcatc
    13381 gcattacgga gtggcgcgcg atctggccgc ctatttaaaa caaagtggaa agccgttttc
    13441 cctgacaaaa ccctctgttg acggtttcaa gacagataaa tcggaaggtg ggatagacgt
    13501 ggtggttgaa aatagtgccg cctgtccaag atattccggg ttgaccgttc gtggtgttac
    13561 cgtaaaggaa agtcccgatt ggttaaaaaa caaactgttg gttatcgggc tgcggccgat
    13621 caacaacatt gtggatatca caaacttcat ccttcatgaa accggccatc cgatgcacgc
    13681 atttgatgtg aattatatcg aaggcaacac ggttgttgtt cgcacattgc ccagaaaaag
    13741 caagttcctt acactcgacg aacaggaacg cgaattgaac gaaaacgacc tgatgatctg
    13801 caacaccaag gaagggatgt gtattgcggg agttttcggc ggactgaagt ccggggtaac
    13861 cgagagcaca acagatgtgt ttctggaatc ggcatatttt aatcctacct gggtgcgcaa
    13921 aaccgctcgt cgtcacggat tgaacaccga ttcatcgttc cgcttcgaac ggggtgccga
    13981 ccccggaaac acggtgtacg tactgaaacg tgccgccatg cttatcaagg agattgccgg
    14041 aggtgaaatt gaaggcgaaa tacgcgatat ctatccggtg aaaataaaca atgcacaagt
    14101 agttttgccg ttccggaagg tgaactcact tatcggaaaa gagattccga aggaaacgat
    14161 taagaacatt ctccaaagcc tggacattga aacggaaaac gagtcggaag aggcactgac
    14221 acttagcatc cccacttaca gagtagatgt gacccgcgat gtggatgtga tcgaagagat
    14281 ccttcgcatc tacggataca acaacgtgga tttcagcgat tcgttaaggg ccaacctgtc
    14341 ttatcaaacc cctacagacc gcaaacacaa actacagaca cttatttcgg aacaacttac
    14401 tgcaagtggc ttcagcgaaa taatgaataa ctcgctcacc cggaaatcct attacgaaaa
    14461 caacacaacc ttcccgggag aaaacggggt aaagctgctc aaccccctga gtaacgacct
    14521 gggagtaatg cgccagacgc ttctttacgg aggcctggag agcatcgctt ataaccggaa
    14581 caggaagcat tacgacctgc atttttacga atttgggaac tgctactttt ttgatactga
    14641 caggaagcag gaaggcaata tattatcggg gtattccgaa gaaacccatc tcggcctttg
    14701 gatttgcggc gacagcaacc ccaaaaactg ggcagcccct caaactgaaa cttctgtttt
    14761 tcaactaaaa gcgcatatcg aaaatatatt gaaccggttg ggctttactc agaatcaggt
    14821 gacgtttgag ccgactcaat ccggaatcta cgcttcagga atgaatatac gtactttcaa
    14881 ccggcagatc ggaagttttg gaattatctc aggcaacatc ttgaagaagt tcgatatcga
    14941 caccgacgtc ttttttgccg aaatgagctg ggatgctctg ttgaaggaaa catcagcaag
    15001 caaagtacat ttttctgaaa tctccaggtt cccctcagta agccgcgacc tggcactact
    15061 tgtcgataaa agcgtcacct ttgcagccat tgagcaaaca gcaaaaaaag cggagaggaa
    15121 actgcttcaa gaactgtctc tgttcgatgt ttacgaagga aagaacctac ctgaagggaa
    15181 aaagtcgtac gcggtaaatt tcgtattgca ggacaaagaa aaaacactca atgataaaca
    15241 gattgacgcc gtcatgcaaa aaattcagca gaatttggag aaagaactgg gagcattgtt
    15301 aagataataa tttttaaaga aagccaattg caaaaatatg ggaagagcat ttgaatacag
    15361 aaaagccaca aagctaaaac ggtggggcaa tatggcccgc gtatttacga aattaggaaa
    15421 acagataacc atcgccacgc gcgacggcgg tcccgatccg gaaaacaatc cacgattgcg
    15481 tgtactgata cagcaggcaa aaaaagaaaa tatgccaaag gaaaacgttg aacgcgctat
    15541 caaaaaagcg acggacaagg acgtttcgga ttacaaggaa atggtttacg aaggttacgg
    15601 cccatacggc gttgccatcg tagtggaaac cgcaaccgac aatcctaccc gaacggtggc
    15661 gaacgtccgg agttacttca acaagcacgg tggatcactg gggacctcgg gaagtctgga
    15721 gtttcttttc gaccacaaat gcgtctttaa aatctctcca aaagaagggg tttcattgga
    15781 agacctggaa ctggaactta tcgattacgg tgttgatgaa gtagaggaag acgaaggaga
    15841 cattatcctt tacggtgaat ttaaatcgta ctcagatatc cagtcgtacc tcgaagaaaa
    15901 cggtttcgaa attcattcgg cagagtttga acgtattccg cacgacacta aatcgcttag
    15961 cgacgaacaa cgcacgcaga ttgaaaaact gcttgagaag tttgaagagg acgacgatgt
    16021 gcaaaacgtt tttcacaata tggaataaac ggaaaagagc aaggaactaa gtttaatcgc
    16081 cctatatcgt ccgtctttgt tctttactcc ttgctctcag aataatacct gtccagggcc
    16141 tccttagccg ccacgaatga gctcttacgc gccgaaagga cttccgactc aagaaggggc
    16201 atcagttgct ctatttcggc attctggtaa aagtcgttcc gcaaacgctc gttgatggtc
    16261 tcatacatcc agaatttcga ctgcttgttg cgacggacat cgaaataacc gctgtccttc
    16321 acaaactcaa tgtaccggtg aatcatatcc catacttctt caattcccag cgcatagtat
    16381 cccgagtagg tcaacacttc tggcgaccat tccgattccg gaagcggaaa cagatgcaag
    16441 gcattgcgaa gctgggactg tgccattttg gccttttcga cgttgtcgcc atcggctttg
    16501 ttgatcacaa taccgtccgc catttccata atcccccgct ttataccctg caactcatca
    16561 cccgtgccgg caagttgaat caacaggaaa aaatcgacca tcgagtggac agctgtttcc
    16621 gattgcccca cccctacggt ttccacaaaa atataatcga accctgcagc ttcacacaga
    16681 acgatggtct cgcgtgtttt acgggccaca ccacccaacg agccggctga aggcgaaggc
    16741 cggataaagg cattctcctg taccgacaat ctttccatcc gagttttatc gccgagaata
    16801 cttcctttgg tgcgttcact cgacgggtct atagccagta cggccagctt atggcctttt
    16861 tctaccagat acatgccgaa cgcatctatg gaagtgcttt tcccagcacc ggggaccccc
    16921 gtaattccta cacggatagc gtttccggaa tacggcaggc acttttcgat aatggcctga
    16981 gccattgcct gatgttccgg tttggaactt tccaccaacg ttactgcctg gctcaacacc
    17041 gtgatgttac ccgccaaaat cccttctgca aactccttca ccgaatattc tttccgttgt
    17101 tgttttttgc gaagatgcaa atagggattt atgcttggtg gctgaactac acccttgttg
    17161 acgtttaaac ccaagtattt agggtcattt tcgggatgat gcataactta aaatactcac
    17221 ctttttctgg gcgtaacatt tccttttatc gacaacacat atacggtgtc aggtacattc
    17281 gtatacacgg ttatggtttt gttaaacgta cccgggcgcg cggtggttga atacttggca
    17341 tcaatcttac cacttttccc aggcaatata ggctcttttg tatagctggg ggtagtgcac
    17401 ccacacgatg cagaaacacg ctgtataatt aacgggctct ttcccatatt ggtaaactcg
    17461 aactgtgtgg aaacagcccc tatttcctct ttaatggttc caaaatcatg aacacttttc
    17521 ttgaattgca ttttcgggcc ggattgtgaa aaagcaaatc cactcaacac tatactgaaa
    17581 aaaaggataa cgcttatttt tttcattttt atctttgttt aaattcgatc atcaattctt
    17641 ttacgacaca aaaataacca tttcgtttaa aaacgctctg tgtttaacgt tatttattgc
    17701 acctatcgga catttacctt cattcccgca gtgtgcgaac tgaactccgg cgcatacatg
    17761 cactgaacgg tggtaattcc attggaataa tcacccgtcc gggtaacgaa agcaccgtat
    17821 tccaaaacgt atgttccccg tggcaagaca tcgaaataga aattagtgga tgcatctttc
    17881 gaagcctgga aatagatgac cccttcctgc catttcatgc ccgaaagttg ttccagaggc
    17941 tcgaaagaag cggcgcgcat atctttcaga tgtacaaatt ccaaatcgcg gtcagtacgg
    18001 agggtcaatc gaacaatcac tttatcgccc acgcgtagca tccggttttc tgtaatcgga
    18061 acgagttttc tacctgaaac gtcggtttcc tccacaaaca acattttttc cacattgagc
    18121 gaacctccgg ttttcgttat tttatccaga tcttcatagt attgccagta gagcgcaccc
    18181 caggccggag cgttacccga attttcaatt ttaacttttc ccatttgagg tgtaatctcc
    18241 gacttatgcc acgtttcttt aaaatatccc gtaccctgtg cctgttgttg gggagcaatt
    18301 accttaccgc ccaccgtcac cgtcgtattg ttgtctgcag aaaaccagtc tgttcccgca
    18361 cttaacaagg catagacagc atctattgtg gcatgcgtgg attcccacat ttgcgtttgt
    18421 ttctgtttca acaaccaacg tttcatatga tccagttcat cggcttttgc accgccaaca
    18481 cggaaagcgt ccataatgaa cgtatgtacc aacacggccg attgcgacat aaaaacatgt
    18541 gcgcggttat ttgcccagaa catgcccatc tcttcggaaa ggacagcgtg ttcgcggtac
    18601 gatctcagca tatcccgtac aacattttgt tttccgtctt tctgcatcaa gacaacgata
    18661 agcgaacgtt cgtacaaccc gaattgtgtc cagtttttct ctactattga tgtataaaaa
    18721 tcggccattt cttttgtttt ggaatccaac ggatattggt tgtaagccga tcggacatac
    18781 aggtattcca agtcggaaac gggaatggtt tttaattttt gccaatcttt gttctgtttt
    18841 ttcagttgct caaaacg
//
LOCUS       DAOSDR010000006        18658 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrimonas sp. isolate AMR_MDS_1826
            NODE_1220_length_18658_cov_4.190238, whole genome shotgun sequence.
ACCESSION   DAOSDR010000006 DAOSDR010000000
VERSION     DAOSDR010000006.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863355
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrimonas sp. (wastewater metagenome)
  ORGANISM  Petrimonas sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Dysgonomonadaceae; Petrimonas.
REFERENCE   1  (bases 1 to 18658)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18658)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:10:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,720
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 35
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18658
                     /organism="Petrimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1220_length_18658_cov_4.190238"
                     /isolate="AMR_MDS_1826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2023866"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1602)
                     /locus_tag="PLI30_00530"
     CDS             complement(<1..1602)
                     /locus_tag="PLI30_00530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019539186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HOI78071.1"
                     /translation="MTTLKRILLLFFILPALGVSAQHYTISGYITDKETGEQLISATV
                     FDELSAKGNATNNFGFFSLTLPKGKVSLAFSYIGYQPEKQSFVLLRDTVVNIQLTLQN
                     VLQEVTVVGHRSETGVRGSQMSAVEVPIAQIKNIPSLLGENDIIKALQLLPGVQSGTE
                     GSAGLYVRGGGPDENLLLLDGIPLYNVNHMMGFFSVFNSDALKNVTLYKGSFPARFGG
                     RLSSVVDVRMKDGDDKKISGTASIGTLSAKLQVEGPIVKEKTTFNVSLRRTYFDILAQ
                     PLIKYAARQEEDDTNYGGGYYFYDINAKFTHKFSDRDRLYLSIYTGDDAMHTKMRTKS
                     ANSSTYESKEWQKMNWGWGNLVTSLRWSRVVGSKLFMNTTAAFTRYRSDLKMGYEDQY
                     VDKSVTPHKTSKSEIALKYDSGIHDWTAKVDFDYTPGPSHDIKFGTNYTYHTFSPDVS
                     SIKVNDTDPNTQKIDTIVGSPNVYAHETMSYIEDNIALGELFKVNLGVHFSSFHVQKE
                     NYFSVQPRAGMRVMLGENFSAKAGYA"
     gene            complement(1899..2840)
                     /locus_tag="PLI30_00535"
     CDS             complement(1899..2840)
                     /locus_tag="PLI30_00535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006799466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogen peroxide-inducible genes activator"
                     /protein_id="HOI78072.1"
                     /translation="MNIQQLEYIIAVDNYRHFSKAAEASFVTQPTLSMMIQKLEDELG
                     VKIFDRTQLPVQPTEIGRKIIDQARVSVAQINQIKEIIQEEKGIIKGTFRLGIIPTVS
                     PYLLPKLMDEHTQGNSDISIVVRELTTDNILRGLANDSLDGGILATPLNDDSVTERPL
                     YYERFFAYVSPKERSLYVKNELDESDLNNGKLWMLDEVHCFRTQILHLCSMRKRRRSN
                     SIFAFEAGSIDTLIKIVDDNDGLTVIPEMATYNLSEQQKKNVRPFKNVTPVREISLIT
                     RKEFIRERLIHIIVDEVKSAVPNSLKDPALKKYVIPL"
     gene            complement(2837..3814)
                     /locus_tag="PLI30_00540"
     CDS             complement(2837..3814)
                     /locus_tag="PLI30_00540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019539313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase-like protein"
                     /protein_id="HOI78073.1"
                     /translation="MVNLQTTFAGLTLKNPFIAASSGLTNSLSKIKELDKAGIGAVVL
                     KSLFEEQIENHTEKLTQISDYPEAADYINAYIQMNHVEKYLDLIRSVKAECTIPVVAS
                     INCYKLTRWTDFAKSIEAAGADAIELNVFLLNAGEYGDTYLEDSYISIVKQLKKTVKI
                     PVVVKMAKNMGNLPGLVGKLKALGTDGIVLFNRFYQLDIDINKMEITAGTVFSNPADF
                     HETLRWTAIVSGRVKEMDILCSTGVHSWEDTIKGILAGASGVQLCSVLYEQGVEVIGN
                     MITCVEEWMEQNNYERISDFKGKLNYANIASPSLYERVQFMKYFSNYQQ"
     gene            complement(3889..4551)
                     /locus_tag="PLI30_00545"
     CDS             complement(3889..4551)
                     /locus_tag="PLI30_00545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019539314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HOI78074.1"
                     /translation="MNIDSNIEKLRVSVPAGVKIVAVSKFHPNEEILEAYNGSQRSFG
                     ESRVQELVEKQPGLPRDIEWHFIGNLQRNKVKFIAPFIHLIHSLDSERLMLEIEKQAA
                     ANHRTIPCLLQLHVADEETKSGFSPDECRRFLARGKWRDCTHVQLAGVMGMATYTDDQ
                     MQVRKEFRLIRSLFGEFKSDYFPDDDHFKEISMGMSGDYPIAIEEGATIIRVGTLIFG
                     ER"
     gene            complement(4548..5000)
                     /locus_tag="PLI30_00550"
     CDS             complement(4548..5000)
                     /locus_tag="PLI30_00550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005855722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4494 domain-containing protein"
                     /protein_id="HOI78075.1"
                     /translation="MYNWFECKVRYDKTLDTGMIKTVTEPYLVDAMSFTEAEARIIEE
                     MKPYISGDFTVSDIKRARFSDTFFNETGDRYYKAKLYFITLDEKSGSEKKTAVNMLVQ
                     ASVLKEAVEIVETEMKKTMVDYTFASVNETAIMDVFKYSAGDNSKAEE"
     gene            5170..5847
                     /locus_tag="PLI30_00555"
     CDS             5170..5847
                     /locus_tag="PLI30_00555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019539316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HOI78076.1"
                     /translation="MIGTLVNTAAVLGGGVIGLLLKKRMPERVTTIYFQAIGLFTLAI
                     GVDMAVEMEKILIVVSSLAIGSLLGEWWNLERGAEQISDNLKKRFRIGSEKFSEGLVT
                     AFLLFCVGSMTILGTIQEGTGGSPDLLFTKSLMDFFSAILLASAFGLGVVFSALPLFI
                     FQAALTLLAGYAGSLFTDEIILGLTSVGGILLIGLGINILGIKKLRIMNMLPSLVVVV
                     LLIWIFG"
     gene            5849..7270
                     /locus_tag="PLI30_00560"
     CDS             5849..7270
                     /locus_tag="PLI30_00560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019539317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HOI78077.1"
                     /translation="MQQKEEKLGLWLLVFVALGSMIGSGIFNSPKDLIRVANPQGTLV
                     AWVTGGLGALMLALVFVYLATRKPGLKSGIYAYARDGFGDYMGFNSAWGYWSVGWLGN
                     VSYLALFFKTLNDLLGERALSPFTAFLIGSALLWFFYAILMAGIREGAILNFIVTTAK
                     LIPILLIIFLGISLVKNDLFLTTDWQSRLASTGEPTSPLLQVKEAMAVILWCFVGVEA
                     ASVLSARAKSQQVVRLSIILSLLIVLAVYMLITFIAMSSVPARELADSQTPLALILER
                     TVIGVAGGTIIKLGIMVSVLGASLSWILLSVETLYAAAKDGVLPQTFRKINRKGTPVN
                     ALLLTQCFTQLFLLSILSPQLNETYLAAITIATTLVLIPYLLSSLYAVKVTLSRWRKE
                     SHHHLVIALLGTLYSLYVIYTVGLKYLFLSVLFYGIGSLLFIAAKREQKQQPKHWEWA
                     VIILLLAGSTGIIAGILTDRIQL"
     gene            complement(7271..9646)
                     /locus_tag="PLI30_00565"
     CDS             complement(7271..9646)
                     /locus_tag="PLI30_00565"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOI78078.1"
                     /translation="MKRLAMIVLAVSQIFISYSRNIEPEKDDNPQDTLKVYYLNEVVV
                     TSSVKETNELKNMPTAVSVVSPKQLRDTQIESLPGLSSFIPNFFIPSYGSKVSTPIYI
                     RGIGARLGAQTVSMYVDNVPSFNPSAFDFEFQDIQRIEVLRGAQGTLYGRNAIGGIVN
                     LYTLSPLTFQGTNLTIGGGSYGQFSAKASNYSKLTDNFGISVGAYYKRDDGYFMNSHT
                     GKKVDASENAGGRVKLDWEITPSFKALLFGNYDYVSGGAFPYMHKDSTAANFNEPSSY
                     NRHLFTNGLSLDYTGNGYSIHSTTGFQYLKDDMKMDQDYSPKSVFSIRQMQEQHSLSQ
                     EITVKSGTQRKYRWVVGAFGFYDHRVIDTPVAIKEEGMAAMQGHLDAAMQRTGAPLRI
                     VYANDRIDLPGIYTKPSRGAALFHQSTLTELFGLEGLSATAGLRLDYEHTGIDFSTES
                     RGGDITVRVPGRDMVIPIDGDTLIEGSFSKSFWKILPKFALKYEFSPTSIIYLSASKG
                     YKTGGYNEQVFSEVLQNALEESLKSSMPPGMGGPPTVPGEEIVKPSLEEQLSYDPETS
                     WTYELGGRYEMFDRKFSLTYALFYSKVNDIQIIELRKQGTTGRTIKNAGKSKSSGFEL
                     SVKYTPVNNFSLYGDYGFADARFVKYDASEDVDYSRNYVPFAPQHTLSLGASYVYDFA
                     NSPLIDRLVGNIQYAGVGKIYWTESNKTTPQNGGSGEELYQPFYGLVNGSISAEKGAF
                     SLELWTKNLFNTDYHSFLFEASDMTTGKVNQFVQRGYPTRFGATLRYTINR"
     gene            9882..10346
                     /locus_tag="PLI30_00570"
     CDS             9882..10346
                     /locus_tag="PLI30_00570"
                     /inference="COORDINATES: protein motif:HMM:TIGR04134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM-associated putative lipoprotein"
                     /protein_id="HOI78079.1"
                     /translation="MKKVVLKTYAKVLTAFFTLLGTITGCDYFEPRCEYGTPSADFVF
                     KGKVVDKSSQKPITDIRIIHKTGYAPANDTVKTNANGEFELKFNDFPGGDHWIYAEDL
                     DGTKNGGLYDSDSLTVNSSQMKRIKKGDGSWYRGVFEKTDANFSLQKSDKKE"
     gene            10343..11467
                     /locus_tag="PLI30_00575"
     CDS             10343..11467
                     /locus_tag="PLI30_00575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008141785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04133 family radical SAM/SPASM protein"
                     /protein_id="HOI78080.1"
                     /translation="MRLRKNMILSLYHEHKKAQAKLHNLTYLFWECTLRCNFNCVHCG
                     SDCAKENIVPDMPKEDFLKILSDIRPHINPHKTMVVITGGEPLVRKDLEETGSEMYNM
                     GYPWGFVTNGWGLSQERLDALLQAGLRSVTVSLDGYTEESHEWFRGKKGSWLRALNAI
                     SRVAGTPGLKYDVVTCVNKKNINDLHKLKSMLVSLEVKHWRLFTVFPKGRAKDNPLLK
                     LSTEEFVQLMEFIRDTRKEGKIIASYGCEGYLADYEMEVRDAPFFCRAGIHIASVLAN
                     GDISACPSLRGDYVQGNIYKDNFWTVWNTRYQVMRDRRWTKTGKCATCKSYKFCQGNG
                     LHLRDEKTGELLHCHLEMMGKLQPAIKQRSIFSKAVSFFT"
     gene            11575..12123
                     /locus_tag="PLI30_00580"
     CDS             11575..12123
                     /locus_tag="PLI30_00580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019539322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family protein"
                     /protein_id="HOI78081.1"
                     /translation="MKKALLIVDVQNDFCPGGALGVKEGDKVVSVINSIIDKFDFVIS
                     SQDWHPEESIHFEKWPPHCIANTYGADFHPDLKTEKIGLKLQKGTANKDDGYSAFEAT
                     NVSFSDFLRKNNITNLYVCGLATDYCVKASALDAVNHGLHTFVITDAIKPVNIHPGDD
                     KKALDEMYRNGCVLVESNALEA"
     gene            12120..12902
                     /locus_tag="PLI30_00585"
     CDS             12120..12902
                     /locus_tag="PLI30_00585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019539323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I and II"
                     /protein_id="HOI78082.1"
                     /translation="MKKDLSLRTVTVMRYILPLREGGSLPALAEADDDFKYVLKFRGA
                     GHGTKMLISELLGGQIAVKLGLNVPELVFARLDVDFGRTEGDEEIQDLLKGSEGLNLG
                     LHYLSGAIAYDPSVKIDPLLASKIVWLDAFITNIDRTFNNTNLLLWHRELWIIDNGAS
                     FYFHHSWKDFEKHALNPFPHIKNHVLLPQATKLDETDRWAKEHLSDHFFLELTRLIPD
                     EWLQWRDTNETPEEIRHRYYLFLKTRLTHSHHFLNEARHARG"
     gene            12889..13272
                     /locus_tag="PLI30_00590"
     CDS             12889..13272
                     /locus_tag="PLI30_00590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019539324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3037 domain-containing protein"
                     /protein_id="HOI78083.1"
                     /translation="MQEVKLYEYAVIRLVPRVEREEFFNIGLILFSKKERYIRLEYYL
                     CTKKFGLMHSGLDYEEVIHHLDAFKQIAAGNKDCGPIALLDIAERFRWLTSVRSSVLQ
                     TSRPHPGKTVDLDKTFVRLFEELVK"
     gene            complement(13288..13653)
                     /locus_tag="PLI30_00595"
     CDS             complement(13288..13653)
                     /locus_tag="PLI30_00595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005854365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family protein"
                     /protein_id="HOI78084.1"
                     /translation="MNYLLTIGLLVCSNIFMIFAWYGHLRLAENSWLSKLPLFGVIVF
                     SWLIAFFEYCFQVPANRIGFEGNGGAFSLVQLKVIQEVITLVVFVVFSSVAFQTPLKM
                     NHLIGFIFLVLAVYFIFKG"
     gene            complement(13711..14754)
                     /locus_tag="PLI30_00600"
                     /pseudo
     CDS             complement(13711..14754)
                     /locus_tag="PLI30_00600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009293255.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide epimerase"
     gene            complement(14813..15784)
                     /locus_tag="PLI30_00605"
     CDS             complement(14813..15784)
                     /locus_tag="PLI30_00605"
                     /inference="COORDINATES: protein motif:HMM:NF013073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HOI78085.1"
                     /translation="MAQQSGMQHIIEQVREKHVPDDAVGEKCWGVIYNSVEKMHSGSS
                     HGSETVSEVLLGMPVRLLDKKGGWRRVQAPDGYVGWVSDAVRTMTESELHEYNKKEKV
                     VVIALYAFSYEKADVKSQTVSDLVIGDMLVLKGEKGRFYRVGYPDGREAYIRQSDAKE
                     LKKWLQDMELTPESIVRVARQFMGIPYVWGGTSFKGLDCSGLTKLVYFLHGVILQRDA
                     SQQVLTGRPVDDNGNFSNLEPGDLLFFGTKATAENPKERVVHVAIYIGNRRFIHASDY
                     VQIGSFDPADPLYDEYNAGRYLCARRIIGEVNTDGIEGVPDNAFYKP"
     gene            16082..16726
                     /locus_tag="PLI30_00610"
     CDS             16082..16726
                     /locus_tag="PLI30_00610"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006744832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate(D-aspartate)
                     O-methyltransferase"
                     /protein_id="HOI78086.1"
                     /translation="MDTSQHPNNEARKLAEGLKRKGISDKRVLAAISNVPRHLFIDER
                     LAEYAYLDRPLPIEKGQTISQPYTVAFQTELLQLKPGEKVLEIGTGSGYQAAVLCEMG
                     ADVYSIERYQELHLQAKETLNKLGYYPRLFFGDGFEGLPQHAPFDKILVTAAPEKTPE
                     KLLNQLKIGGWMVAPIGGRQGQRMTVIKRISNDKFRESEHGDFIFVPMQKGTDE"
     gene            16768..>18658
                     /locus_tag="PLI30_00615"
     CDS             16768..>18658
                     /locus_tag="PLI30_00615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019538642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOI78087.1"
                     /translation="MNRQKQFSDRKTFRFKRFARRSYSVFNSIHKSVTIGVLSGCALL
                     SAHAASVNPTEKTGIQAKPDTIPVKELDEVVVTASKAPLPLNMAAKLVTVITSKEIER
                     APVRSTEDLLNYVAGVDILQRGPHGVQADISLRGGSFDQAAILLNGVNLTNPQTGHYS
                     FDIPINLSDIERIEIVQGPSSLIFGAGAFSGGVNIITKKDTHSNAFFKTEGGMHGLFG
                     AEARGAYKTANSVHRLSAGYKHSGGYIKNSDYDIVNLLWQSRYNFDNSTIDVQAGVND
                     KKYGANTFYTAAYPQQYDDTRGVFASVKGETGGKLKFIPQLYWSRHYDVYHLFRPGTT
                     DIPSWYTTPNYHRSDVYGLNLNMQYASDLGITSFGGEFRNEGILSSNLGRLMRQKSGN
                     YDKWDNRSNISFFVEHNFVFDKLTLSVGGLYNYNTALADTLKANGFYPEINVSYRTND
                     AVSLYASWSKSTRMPTFTDLYYKAGKHRGFAGLTPEYSQSVEIGWRYKNSVLTANLNV
                     FYTKGDNLIDWILNPLDGFYHAQNLASLGTYGAGWDATVNMHQWLGEQQPLQSIQIGY
                     QYMSQKSDEARQNNPVSAYVFNYLRHKFTVGVQHRIVKNLSLSWNLRWQDRAGNYLKY
                     ITEIKE"
BASE COUNT         5038 a         4513 c         4114 g         4993 t
ORIGIN      
        1 ggcgtaaccg gcttttgctg aaaagttttc gcccaacatt acccgcattc ccgcacgcgg
       61 ttgcaccgag aagtaatttt ccttttgcac gtggaacgac gaaaaatgca cacccaggtt
      121 caccttaaac aattccccca acgcaatatt gtcttcgata tagctcatgg tctcgtgcgc
      181 atacacgtta ggacttccca caatcgtatc gattttttgc gtattggggt cggtatcgtt
      241 tacctttatc gacgacacat cgggtgaaaa cgtgtgatac gtgtagtttg tgccgaactt
      301 gatatcgtgc gacgggccgg gcgtataatc aaagtcaacc ttagccgtcc aatcgtggat
      361 acccgaatcg tatttcagag caatttccga tttactcgtt ttatggggcg tcacggattt
      421 atccacgtat tgatcttcgt agcccatttt caggtcagac ctgtatcgcg tgaatgccgc
      481 agtagtattc ataaacagtt tgctgcccac cacacggctc caccgtaatg acgtaaccag
      541 gttgccccaa ccccagttca ttttttgcca ttctttgctt tcatacgttg aactattggc
      601 agattttgta cgcattttgg tatgcatggc atcatctccc gtgtaaatac tgagatagag
      661 tcggtcacgg tcggaaaact tgtgtgtaaa tttggcgtta atgtcataaa aataatatcc
      721 gccaccgtaa ttcgtatcgt cctcctcctg cctggcggcg tacttgataa gcggctgggc
      781 caaaatatcg aagtaagtac gacgcaacga aacattaaat gtggtttttt ctttcacgat
      841 gggcccttcc acctggagtt tcgccgaaag tgtcccgatg ctggctgttc cgctgatctt
      901 tttatcgtcg ccgtccttca tgcgcacatc caccacggag gagagccgtc ctccgaaacg
      961 tgccgggaag ctacctttat aaagcgttac gtttttaagc gcatcactgt tgaaaacgga
     1021 aaaaaagccc atcatgtgat tcacgttgta gagcgggata ccgtccagga gtaacaaatt
     1081 ttcatcgggc ccaccgcccc tcacatacaa accggccgat ccttctgtgc ccgactgcac
     1141 gccgggcaac agctgcaagg ctttaatgat atcgttttca cccaataacg atggaatatt
     1201 tttgatttgt gcgatgggca cttccacggc gctcatttgc gaaccgcgta ctcccgtttc
     1261 ggagcggtgc cctaccacgg tcacttcttg caacacgttt tgcaaggtga gttgaatatt
     1321 gacaaccgtg tcgcgtagca acacaaacga ttgcttttcg ggctggtaac cgatatagga
     1381 aaaggccaaa gaaaccttgc ctttaggcaa ggtgagggaa aaaaagccaa aattgttcgt
     1441 ggcgtttcct ttagccgata attcgtcaaa gacggtagcg gaaatcagtt gctcgccggt
     1501 ttccttgtcc gtaatatatc cgctaatggt gtaatgctgt gccgacacac ccagcgccgg
     1561 caaaataaaa aacaaaagta agatcctttt taatgtcgtc atacccttta gttatggtgt
     1621 tggaattttg ataagcttga aattgttttg ccgaaaggta tatgtgtctc ggtacagcaa
     1681 tgatgcaaag ctgcacaaaa tcacggcacg atacttagtt aaaaaaataa aataaaaggt
     1741 gtaatttcgt tctaatggta cttatctctt cttctcatcc acccaaactt tcaaggtttt
     1801 aggagaatcc gtcacaaaac caccatccgc tataaaaaaa gaaagcaata tttcgctatt
     1861 tatacctgac tgtgttaaag aagcgtcgcg gcctgtctct acaacggaat cacatatttt
     1921 ttcagtgcag gatctttgag tgaatttgga acggcgcttt ttacttcatc cacaatgatg
     1981 tgaattaacc gctcccgtat aaattctttc cgggtaataa ggctgatttc gcgtacggga
     2041 gtaacgtttt taaacggccg cacatttttc ttttgttgtt cactgagatt atatgtcgcc
     2101 atttcgggaa taacggtcaa cccgtcgttg tcatccacta tttttataag cgtatcgatg
     2161 ctacctgctt caaaggcaaa aatggaattc gacctgcgtc tttttctcat gctgcagagg
     2221 tgtaaaatct gtgtccggaa acaatgaact tcatcaagca tccaaagctt cccgttgttt
     2281 aaatcactct cgtccagctc atttttaacg taaagcgaac gttctttagg ggaaacataa
     2341 gcaaaaaacc tttcgtaata gaggggcctc tcggtaacgg agtcatcgtt gagcggagtg
     2401 gccaggattc caccgtccag cgaatcgttt gccaggccgc gaaggatatt atcggttgtc
     2461 agctccctga cgactatgct tatatcggaa tttccctgcg tatgttcgtc catcaatttc
     2521 ggcagcaggt acggcgaaac agtcggaata attcccagcc tgaatgttcc ttttataatg
     2581 cccttttctt cctggataat ctctttaatc tggtttatct gagcgaccga aacacgtgcc
     2641 tgatcgatta tttttcttcc tatctcggta ggctgaaccg gaagctgggt acggtcaaaa
     2701 attttcactc ccaactcatc ttccagcttt tgaatcatca tactgagggt gggttgcgta
     2761 acaaatgagg cttcggccgc tttggaaaaa tggcgataat tgtcaactgc aattatatac
     2821 tcgagttgct ggatgttcat tgctgataat ttgaaaagta cttcataaac tgaacgcgtt
     2881 cgtacagcga cgggctcgca atattggcat aattaagttt tcctttaaaa tcggatatac
     2941 gctcgtaatt attctgctcc atccattctt ccacacacgt tatcatgtta ccaatcacct
     3001 ccaccccctg ctcgtaaagc acgctgcaaa gctgtactcc cgatgcgcca gccaaaattc
     3061 ctttaatggt atcttcccag gagtggactc cggttgaaca aagtatgtcc atctctttaa
     3121 cgcgtcccga gacgatggca gtccagcgca gtgtctcgtg aaaatcggcc gggttgctga
     3181 aaacggttcc ggctgtaatc tccattttgt tgatatcaat atcaagctga taaaaccggt
     3241 taaaaagcac aattccatct gtacccaatg ctttcagttt accgaccagg ccgggtaaat
     3301 tacccatatt cttggccatt tttaccacaa cagggatttt caccgttttt ttcaattgct
     3361 tcacgatgct gatgtaggaa tcctccaaat aggtatcccc atattctccg gcgttgagca
     3421 aaaacacatt caactcaata gcgtccgccc cggcagcctc aatacttttg gcgaaatccg
     3481 tccatcgggt cagtttataa cagttgatgc ttgccacaac ggggatagtg cattctgctt
     3541 taaccgaacg gatcaggtcg aggtatttct ccacgtggtt catctggata tacgcgttga
     3601 tatagtccgc tgcctcggga taatcagaga tttgtgtcag tttttcggtg tgattttcta
     3661 tttgttcttc gaacagggat ttaagcacca ccgctccaat tccggcttta tcgagctctt
     3721 ttatttttga caaggagttt gttaaaccgc tgctggcagc aataaacggg ttcttcagcg
     3781 tcaatccggc gaatgttgtt tgtaagttta ccatgttgag tgaatgtttt ttcaattatc
     3841 aagcgggaca aaaataacta ttttaattga taaaagtgat tgtttatatt acctttcccc
     3901 aaaaatcaac gttcccaccc ggatgatcgt agcaccttct tctatagcaa tggggtagtc
     3961 gccagacatt cccatggaaa tctctttaaa atgatcgtcg tcggggaaat aatccgattt
     4021 gaactcaccg aacagcgatc gaatcaaccg aaactctttt ctcacctgca tctggtcgtc
     4081 tgtataggtg gccataccca ttacaccggc aagttgcaca tgtgtgcagt cgcgccactt
     4141 gccccgggca agaaaacggc ggcattcatc gggtgagaag cccgattttg tctcttcatc
     4201 ggctacatgt agctgcaaca aacaaggtat tgtccgatga ttagctgcgg cctgcttctc
     4261 aatttcaagc atcaaccgtt cactatccaa actgtgtata aggtgaataa atggagcgat
     4321 aaacttcacc ttgttacgct gcagattgcc tataaaatgc cattcgatat ctctggggag
     4381 acccggttgt ttttcaacaa gctcctgcac acggctttca ccgaaactcc gctggctacc
     4441 gttgtaggct tccaaaatct cttcattggg atggaacttg gaaacggcta caattttaac
     4501 acccgccgga accgataccc gcagtttttc gatattggaa tctatgttca ttcctccgct
     4561 tttgagttat cccctgcact atatttaaac acatccatta tggctgtctc gtttaccgac
     4621 gcaaaagtgt aatccaccat cgtctttttc atttcggttt ccacgatctc caccgcttct
     4681 ttaagcactg aggcctgcac gagcatattg actgctgttt tcttttccga accgcttttc
     4741 tcgtcaagcg tgatgaagta aagctttgct ttgtagtaac ggtctcccgt ttcgttaaaa
     4801 aaagtatcgg agaacctcgc ccttttaata tcggaaacgg taaaatcgcc cgatatatag
     4861 ggcttcatct cttcaataat cctcgcttcc gcttcggtga acgacatcgc gtccaccaga
     4921 taaggttcag ttaccgtttt tatcataccc gtatccaggg ttttatcgta ccgcactttg
     4981 cattcgaacc agttgtacat attgtttttt aattttatat aggatcacaa aggtacaatt
     5041 ttttttacag ttttgctttg aaacgatcgt tgtggaagtt tacgtacctc gattttttag
     5101 tcgataaaaa gtaactatcg ggcctttgtc gtgaaaattt gatatttttg caatttattg
     5161 gctaacgaga tgattgggac ccttgtaaat acagctgccg tactgggtgg cggagtgatc
     5221 gggctgctgt tgaaaaaacg gatgccggag cgtgtgacca ccatttattt tcaggcgata
     5281 ggcctcttta cgctggctat cggcgtggat atggctgttg agatggagaa gattctcatt
     5341 gtggtgagca gccttgccat cggctctctt ttgggagagt ggtggaacct ggaaaggggt
     5401 gctgaacaga tcagtgacaa cctgaagaaa cgctttcgta ttggaagcga aaaattttcg
     5461 gaggggctag tcaccgcctt tttactgttt tgcgtgggct ccatgaccat cctcggtact
     5521 atacaggaag gcactggcgg ttcacccgac ctgctcttta cgaaatcgtt gatggatttt
     5581 ttctcggcta ttctgctggc ttcggccttt ggcctgggag tagtattttc ggctttgccc
     5641 ctgtttatct ttcaggctgc cctcacccta cttgccggat acgccgggag cctcttcacc
     5701 gacgaaatca tcctcggact gaccagcgtg gggggtatac tgttgatcgg actgggtatc
     5761 aacattcttg ggataaaaaa actccgcatc atgaatatgc tgccctcgct ggttgtcgtc
     5821 gtgctgctga tctggatttt tggataacat gcaacaaaaa gaggagaaac tgggattatg
     5881 gctactggtt tttgtggcgc tgggttccat gataggctcc ggtatcttta attctccgaa
     5941 agatcttatc cgggtggcca atccgcaggg caccttggtt gcctgggtga ccggagggct
     6001 tggcgcactt atgcttgcac tggtgttcgt atatcttgcc acgcgtaagc ccggattaaa
     6061 aagcggcatc tacgcctatg cacgggatgg attcggtgat tacatgggct tcaactcagc
     6121 ctggggctat tggtctgtag gctggctggg gaatgtgagt tatctggcac tctttttcaa
     6181 gacgctgaac gacctgttgg gagaacgtgc actttcaccc ttcaccgctt ttctaatagg
     6241 gtcggcactg ctgtggttct tctatgccat tctgatggcc ggcatccgcg agggagccat
     6301 ccttaatttt attgtgacaa ccgctaaact gatccctatt cttctgatta tattcctcgg
     6361 tatatccctg gtaaagaatg atctttttct aaccacagac tggcaaagca ggctggcatc
     6421 cacaggcgaa cccacctctc cccttctaca ggtgaaggag gccatggcgg ttatcctctg
     6481 gtgttttgtc ggtgtggaag cagcttccgt cctgtcagcc agggcaaaaa gccagcaggt
     6541 cgtacggctg agcatcattc tctccctgtt gattgtcctt gctgtgtata tgctgattac
     6601 ctttattgcc atgtcatcgg tcccggcccg ggagctggcc gactcacaga caccgctggc
     6661 cctgatactg gagcgaacag ttatcggggt tgcaggcggc acgatcatta aattggggat
     6721 catggtctcc gtgctgggag ccagcctctc ctggatactg ttatccgttg agacattata
     6781 cgccgcggct aaggacggcg tgttgcccca aaccttccgg aagataaacc ggaaaggaac
     6841 ccccgtcaat gctctcctgc tcacacaatg cttcactcag ctttttctcc tttccatcct
     6901 gtcgccacaa ctcaacgaaa cctaccttgc cgccattacc attgccacca ccctggtgct
     6961 gattccctac ctgctctcat cactttatgc cgtgaaagtt accctaagcc gatggaggaa
     7021 agagtctcac caccacctgg tcatagcact actcggcacc ctctattctc tctatgtgat
     7081 ctatactgtg ggcctaaaat acctgttcct ttccgttctc ttttacggta tcggctcact
     7141 gctgtttatt gctgcaaagc gagaacaaaa acaacagccc aaacactggg aatgggctgt
     7201 cattattctt ctcctggcag gatcaacagg gataatcgca gggatcctga ccgacaggat
     7261 ccaactttga ctatcggttg atggtgtacc gcaaggtagc tccgaaacgg gtaggatatc
     7321 cccgttgcac aaactggttc acctttccgg tggtcatgtc cgacgcttcg aaaagaaacg
     7381 aatggtaatc ggtattgaac aggttctttg tccagagttc aagcgagaac gcacccttct
     7441 ccgcagaaat gctgccgtta accaatccgt aaaaaggttg ataaagctct tctccactac
     7501 cgccgttttg aggggtggtt ttattcgact ctgtccagta aattttcccg actccagcat
     7561 actgaatgtt tcctaccagg cgatcaataa gcggactgtt ggcaaaatca tacacataac
     7621 tggctcccag gcttagtgtg tgttgtggag caaagggaac ataatttctg gaataatcca
     7681 catcctccga agcatcatat ttcacgaatc gtgcatcagc aaatccataa tcaccgtata
     7741 acgagaaatt gttcactggg gtatatttca cactcagttc gaaaccgctg cttttcgatt
     7801 tcccggcatt ttttattgtt cgtccagtgg ttccctgttt ccttaattcg attatttgta
     7861 tatcgtttac cttcgaatag aagagggcat acgttaaaga aaacttccta tcaaacattt
     7921 catatcgacc tccaagttca tacgtccaac tggtttccgg atcataggac aattgctcct
     7981 ctaaagaggg ttttactatc tcctcaccgg gtacggtagg tgggccaccc attcctggag
     8041 gcatactgct ctttaaagac tcctcaagag cattttgcag aacttcagaa aagacttgct
     8101 cattgtaacc tccggttttg tatccttttg aagcagaaag atagattata gaggtgggtg
     8161 aaaattcata cttcaatgca aatttgggaa gtattttcca aaaactcttg ctaaaagatc
     8221 cttcaatcag ggtgtctcca tctatgggaa taaccatatc cctgcccggt actctaacgg
     8281 ttatatctcc accccggctt tccgtggaaa aatcgatgcc ggtgtgctca taatccagcc
     8341 ttaaccctgc agtggcagac aacccttcta atccgaaaag ctccgtaagc gtggactggt
     8401 ggaagagcgc agcaccccgg gatggttttg tataaatacc gggaagatca atcctgtcgt
     8461 tggcgtatac aattctcaac ggagcacccg tccgctgcat agccgcatcc aggtgtccct
     8521 gcatggcagc cattccctcc tctttgatgg cgacgggcgt atcgatcacc cggtggtcat
     8581 agaacccgaa ggcacccact acccagcggt atttccgttg tgtgccggac ttcacggtaa
     8641 tctcctggct cagggagtgt tgttcctgca tctgacgaat ggagaatacc gatttgggag
     8701 agtaatcctg atccatcttc atatcgtctt tgaggtattg aaatcctgta gtagagtgta
     8761 tactgtagcc gttcccggta taatccaacg acaaaccgtt tgtaaacaaa tgacgattgt
     8821 aggaggaggg ctcgttaaag tttgctgcgg tggaatcttt atgcatatac gggaaagcac
     8881 ctccggaaac atagtcgtaa tttccgaaga gcaacgcttt aaatgatgga gtaatctccc
     8941 aatccagctt cacccttcct ccggcatttt cagaagcatc tactttttta ccggtatggc
     9001 tattcatgaa atacccatca tcacgcttgt aataggcacc cacggagatt ccgaagttat
     9061 ccgtcagctt gctgtagtta gatgcttttg ccgaaaactg gccgtaactt ccaccgccta
     9121 tcgtcaggtt agtaccctga aaagtgagcg gcgacagcgt gtagagattc actattcccc
     9181 caatggcatt tcgtccgtag agtgttccct gggcacctcg gagaacctct atgcgttgaa
     9241 tatcttgaaa ctcaaaatcg aatgccgaag gattaaagct gggcacatta tccacgtaca
     9301 tgcttaccgt ctgcgctccc aatcttgctc cgataccacg gatgtagatg ggagtagaga
     9361 ctttggagcc gtaagaaggg ataaagaagt tggggataaa tgagcttaag cccggcaacg
     9421 attcaatctg agtatcgcgc aactgtttcg gagaaaccac agatacggca gtgggcatat
     9481 tcttcaactc gttggtttcc tttacagatg aggtaacaac cacttcgttc aggtaataca
     9541 ctttaagcgt atcctgtggg ttatcgtctt tttcaggttc aatattcctg gagtatgaaa
     9601 taaaaatctg gctgactgcc aatacaatca ttgctaatct tttcatcgga caaaaaatat
     9661 actgttaaat tatcatgcaa agtaacacga taatcgaaaa cgaaacaatc accacaaata
     9721 gtgatttttt tcatcaatat ggagaaaatc agccaaaatg tctgtttttt tgaaaatttt
     9781 tcgatttttg atgcaacgtt ttggcgtttt ttccatctat taggaaacag ttttgatttt
     9841 ttatcgttta aatcaagtac acccaagtaa atgatgattc tatgaaaaaa gttgtattga
     9901 aaacctatgc aaaagtattg actgcatttt tcactttact gggaacaata accggatgcg
     9961 attattttga accccgatgc gaatacggaa caccttccgc agacttcgtg tttaaaggaa
    10021 aagtggtcga caaatcatct caaaaaccga taacagatat ccggataatc cataagacag
    10081 gctatgcacc ggcaaacgac acggtaaaaa ccaacgccaa tggcgaattt gagttgaaat
    10141 tcaatgattt tccgggagga gaccactgga tttatgccga agatttagac ggaacgaaaa
    10201 atggcggatt gtatgactcg gactccctga cggttaactc atcgcaaatg aaaagaatca
    10261 agaaagggga tggaagctgg tatcgaggtg tttttgaaaa gaccgatgca aacttctctt
    10321 tacaaaaatc ggacaagaaa gaatgagact ccggaagaac atgatcctct cgctctatca
    10381 cgagcataaa aaggcacaag ccaaactgca caacctcact tatctgtttt gggaatgcac
    10441 gttgcgttgc aatttcaatt gcgtgcattg cggaagcgac tgtgccaaag agaacatcgt
    10501 tcccgacatg cccaaggaag atttcctgaa gatactgtcg gatatccgcc cacacataaa
    10561 tccgcacaaa acaatggtcg tcattaccgg tggggaaccg ctagtacgaa aggatctcga
    10621 ggaaacaggc agtgagatgt acaacatggg atatccgtgg gggttcgtca ccaacggctg
    10681 gggactgtcg caagagcggc tcgatgcgct tctccaagcg ggactacgtt ccgtaaccgt
    10741 aagcctggac ggatacaccg aagagtcgca cgagtggttt cgcggcaaaa aaggatcgtg
    10801 gctcagggca ctgaacgcca tttcacgggt agccggaacg ccgggattga aatacgacgt
    10861 ggtgacgtgc gtaaataaaa agaacatcaa cgatctccac aagttaaagt caatgcttgt
    10921 ttcgttggaa gtcaaacact ggcggctgtt taccgttttt ccgaaaggaa gagccaaaga
    10981 taacccgtta ttgaaattat ccaccgaaga atttgtgcaa ttgatggagt tcatccgcga
    11041 cacccgaaaa gaagggaaaa taatcgccag ctacggatgc gagggatacc tggctgatta
    11101 cgagatggag gtgcgtgacg cacccttctt ttgtcgggca ggtattcaca ttgcttcggt
    11161 gttagcaaac ggggacatca gtgcgtgtcc cagcctccgg ggcgattatg tccagggcaa
    11221 catctacaaa gacaatttct ggacggtttg gaacacccgc taccaggtga tgcgcgaccg
    11281 ccgatggacc aaaaccggaa aatgcgccac ctgtaaatcc tataaattct gtcaggggaa
    11341 cggattacac ctccgggatg aaaaaacggg agagttacta cactgccatc tggagatgat
    11401 ggggaaatta caacccgcca taaaacaaag gagcatcttc tccaaagccg tgagtttctt
    11461 tacgtgatcg ggggtgaacc caaatgcttt ttctttgtta tagaggttgg aatcacgaaa
    11521 gtattagcga tttcggctct tgattctttt atctaaaaag tctataaaaa aacaatgaaa
    11581 aaagcattat tgatcgtgga tgtccagaac gatttttgtc ccggcggcgc actgggcgta
    11641 aaagaggggg acaaagtggt ttccgtgata aacagcatta tcgataaatt cgattttgta
    11701 atttcatcac aagactggca ccccgaggaa tccattcatt ttgagaagtg gcctccgcac
    11761 tgcatcgcca atacgtacgg tgctgatttc catcccgacc tgaaaacaga aaagatcggt
    11821 cttaaactac aaaaaggcac ggccaacaaa gacgacggct actccgcttt cgaagcgacc
    11881 aacgtttcct tttccgattt tctccgcaaa aacaacatca ccaacctgta tgtgtgtggg
    11941 ttagccaccg attattgtgt aaaggcatcg gctctagatg ctgtaaacca cggcttacac
    12001 acatttgtga taaccgatgc tataaaaccg gtgaatatac atccgggcga cgataaaaag
    12061 gcattggatg aaatgtaccg gaacggatgc gtactggtag agtctaacgc gttggaagca
    12121 tgaaaaagga tctttcactc cgcaccgtaa ccgtaatgcg ttatatcctg ccgttgaggg
    12181 agggtggttc gttgccggcg ttggcggaag ctgatgacga tttcaagtat gtgttgaaat
    12241 ttcgcggtgc ggggcacggc accaaaatgt tgatttcgga gttgctgggc ggacaaattg
    12301 ccgtcaaact cggattaaat gttcccgaac tggtgtttgc ccgtctcgat gtggatttcg
    12361 gacgaaccga aggagatgaa gaaattcagg atttgttaaa aggaagcgaa ggacttaatc
    12421 tcggcctgca ttatctttca ggagcgattg cttacgaccc atctgtaaaa atagatccgt
    12481 tgttggcgtc gaaaattgtg tggctcgatg catttattac caatatcgac cgtaccttta
    12541 ataacaccaa cctgttgttg tggcatcgtg aattatggat catcgataac ggcgcgtcgt
    12601 tctattttca ccattcctgg aaagatttcg agaaacacgc gctcaacccc tttccgcaca
    12661 tcaaaaatca cgtcttgttg ccgcaggcta caaaactgga tgaaacggac aggtgggcaa
    12721 aggaacattt atcggatcat tttttcctcg aactcacccg gcttatccct gacgaatggt
    12781 tgcagtggcg tgatacgaat gagactcccg aagaaatccg ccacagatac tacctttttc
    12841 tgaaaacacg attgacgcat tctcatcact ttttaaacga agcccgacat gcaagaggtt
    12901 aagttgtacg aatatgccgt catccgcctg gtgccaagag tggagaggga agagtttttc
    12961 aatattgggt tgattctttt ctcgaagaag gaaaggtata tccggctgga gtattacctc
    13021 tgtacgaaga aatttggatt gatgcactcg ggtctggatt acgaggaggt tatccaccac
    13081 ctcgatgcat ttaagcaaat cgccgcaggc aacaaagatt gtggccccat tgcgcttttg
    13141 gatatcgccg agcgattccg ctggctgacc tccgttcgca gttccgtatt acagacatcg
    13201 cgcccgcatc ccggaaaaac cgttgacctc gataaaactt ttgtgcggct ctttgaagag
    13261 ttggttaaat aagtgccaaa gtcctcttta ccctttaaaa ataaaataga ctgccagtac
    13321 cagaaatata aaccctatca ggtgattcat cttcaatggg gtttgaaaag caaccgaaga
    13381 gaagacaaca aagaccacca atgtgataac ctcctgaata accttcagct ggacgagcga
    13441 aaacgctccg ccgtttcctt caaacccgat ccggtttgcc ggaacctgaa agcagtattc
    13501 aaaaaaagcg attagccagc tgaaaacaat tacaccaaaa agcgggagtt tgcttaacca
    13561 cgaattttca gcaagtctga ggtgcccgta ccacgcaaaa atcataaaga tattggagca
    13621 gaccagaagc ccgatagtga gaagataatt cataaccgtt acttaaaaac cgggcgcaaa
    13681 agtaatgaaa atccaggtta aaacagcctc ttaacgggta aaatttattt tctgattggg
    13741 cactacacca attcccgaca gatcgttcgc gataattttg ccgttctgca aacgggctcc
    13801 gtcaaaacaa tcgttagcaa tcagcagggc tccgtccaaa tcggcaaaat ccattcctga
    13861 acaaagttga gatgcagcag aaatagcaca tgacgtttca gtcatacatc ccatcatcac
    13921 tttcattccc aatgactcgg caactctacg catcttccag gcctcgcgca tgccggtaca
    13981 tttcatcaac ttgatgttaa tgcccgagaa cacccctttc aaccgctcga catcggttaa
    14041 ccgctggagt gactcgtcgg caaaaacagg cagagggctc tcttccgtca atcgggaaat
    14101 attgtccaga tccgacttcg gcattggctg ctctatcatt acaacccctt ggtccttcat
    14161 ccagtgaatc atgtcgagtg ccgcttgacg gtctttccaa ccctggttag cgtctaccgc
    14221 caacggcaga ccggtaacgg aacggatggc ttcaatcatg cgcttgtcgt ccgggccgcc
    14281 cacttttacc ttcaagatgt taaagcgtcc cattgcttcg cgtgtttttt cacgcaccac
    14341 atcatcggta tcaataccga tggtaaaagt agtatcggga acatccattc tgtccagtcc
    14401 atacctctta taccacgtta cgccggcaat tttcccggct aaatcatgca acgctatgtc
    14461 cacggaagct ttggccgccg tattgttaac cgaaatacca tccacatatt ctagaatctc
    14521 atccagacat aagggatcgg ggaaggcaga cagatcgact ttcttcagga attcaataac
    14581 cgaagcctgc gtctctccca agtacggagg tagcgacgcc tcaccgtaac ccgtaagccc
    14641 gtcgtattca agtttagtga gtacaaccgg cgttgtttta cgggagaaac ccgatatggt
    14701 aaacgtgtgg cgcagttcaa ggtcgtaagg agcccagaat aaattcattt tggaagaact
    14761 gtttttttcg ttgtcattgt cgctgtctgg ataaaattct gcctgcctag attcatggtt
    14821 tgtaaaaagc attgtccgga actccttcga taccgtccgt gtttacttca ccgataattc
    14881 ttcgggcaca cagataacga ccggcattat attcatcgta aagcggatcg gcaggatcaa
    14941 aactgcctat ctgaacataa tccgacgcat gtataaaccg tcggttcccg atataaatgg
    15001 ctacgtggac aaccctttct ttcggatttt ccgccgtcgc cttcgtcccg aaaaagagca
    15061 aatcacccgg ttccaggttt gaaaaattgc cgttatcatc caccggtctt ccagtcaaca
    15121 cctgctgcga agcatcccgc tgaagaataa ccccgtgtag gaaatagacc agtttggtca
    15181 acccactgca atccaaaccc ttgaatgaag tgccgcccca aacgtaagga attcccataa
    15241 attgcctagc gaccctcact atgctttccg gggtaagctc catatcctgt aaccacttct
    15301 tcaactcctt ggcatcggac tgccgtatat aggcttcacg cccatcggga taccctaccc
    15361 gataaaaacg gcctttctct cctttcagta caagcatatc cccgatcact aaatcggata
    15421 ccgtttgcga tttaacatcc gctttttcgt aggaaaatgc atacaacgca attactacca
    15481 ccttttcttt tttgttgtat tcatgtagct ctgactcagt cattgttctt acagcatcag
    15541 aaacccatcc gacgtatccg tccggtgcct gcacgcgtcg ccaaccaccc tttttatcca
    15601 ggagcctgac tggcatcccc agcaaaactt ccgatacagt ctcactgcca tgggaactgc
    15661 cggagtgcat tttttctacc gaattgtaga tcactcccca acatttttca ccaacggcat
    15721 catcgggcac atgtttttcc ctgacctgtt ctataatgtg ttgcataccg ctttgctgag
    15781 ccataaaaca taatgttgaa atatctacaa aaggaacaaa gaaattactt ttccatcctt
    15841 tgccggataa attaacagag cgaatttaca atttttattt caattgaagc attataccgg
    15901 caaaatttag tataaaatac agaaaatcac tatctttgat ctcagaagaa aaggaggaaa
    15961 atacatgaaa acaaggataa aaaaagacct gtcctttatt ttaattggct tttgctgtgc
    16021 aggatcagtc gcttgtgggc aacctcaaaa aaaacaaagt gaaaaaaccg aaaatcgtgt
    16081 aatggacaca tctcaacacc ccaacaatga agcaagaaaa cttgccgaag gacttaaaag
    16141 aaaaggcatt tccgataagc gggttttagc tgccatcagt aatgttccca gacacctttt
    16201 cattgatgaa cgtttagcgg aatatgccta tctcgaccgt ccactgccta tcgaaaaagg
    16261 acagaccatt tcccaacctt acacggtagc ctttcagacg gaactgctac agctgaaacc
    16321 aggcgaaaag gtactggaga ttggtacagg aagcggatat caggcagccg tactgtgtga
    16381 aatgggtgct gatgtttaca gcatagagcg ttatcaggag ttacaccttc aagcgaaaga
    16441 aacactcaat aagctgggtt attatccccg tttatttttc ggtgatggtt tcgaggggct
    16501 acctcaacat gcaccttttg acaaaatatt ggttacggca gctcctgaaa aaacccctga
    16561 aaaactgttg aatcagctca aaataggtgg ctggatggtg gcacccatag gcggccggca
    16621 agggcaaaga atgacggtta taaaacgcat aagcaacgat aaattcaggg aatccgaaca
    16681 cggcgatttc atttttgtcc ctatgcaaaa aggcacggac gaataaaatg cactatcttt
    16741 gtcgcttcat ttctaaaatt ttataaaatg aacagacaaa aacaattttc agaccggaaa
    16801 acgtttcgtt ttaaacgatt tgcccgaaga tcgtacagcg ttttcaacag tattcacaaa
    16861 tccgtcacca tcggtgtact ttcgggttgt gcattattaa gtgcccacgc cgcatcggta
    16921 aatcctacgg aaaagacggg tattcaggca aaaccggaca ccattcccgt aaaagaactg
    16981 gatgaagtgg tggttaccgc ttcaaaagcc ccactgccac tgaatatggc agccaaactg
    17041 gtaaccgtaa tcacgagcaa ggagattgag cgggcgcccg tcagaagcac cgaagacctt
    17101 ctcaattacg tagcaggcgt agatatcctg caacgcggac cgcacggcgt acaggcggac
    17161 atatccctcc ggggtggctc attcgaccag gccgccattt tacttaacgg tgttaacctt
    17221 accaatcccc agaccggaca ttacagtttc gacattccca tcaacctttc cgatattgag
    17281 cgtatagaaa ttgttcaagg cccatcatcg ttgattttcg gcgccggcgc attttcaggt
    17341 ggagtaaaca tcattacaaa gaaagacacc cactcaaacg ctttcttcaa aacggaaggt
    17401 ggaatgcacg ggcttttcgg cgcggaagca cggggggcat acaagacggc caattccgta
    17461 caccgtttgt cggcaggcta caaacactcc ggcggatata tcaaaaacag cgactacgac
    17521 atcgtcaacc tactgtggca aagccggtac aacttcgaca actccacaat cgacgtccag
    17581 gcgggagtga acgataaaaa atacggtgcc aacacgtttt acacggccgc ataccctcaa
    17641 caatacgacg acacacgtgg agtattcgca tcggtaaaag gcgaaacggg tggaaaactt
    17701 aaatttattc cgcagttgta ttggagcaga cattacgatg tgtatcattt gttcaggccc
    17761 gggacaaccg acataccatc gtggtacaca acacccaact accaccgcag cgatgtttat
    17821 ggccttaact tgaacatgca atatgcttcg gatctgggta taacaagttt cggaggcgaa
    17881 ttccgcaacg aaggcatctt gagcagcaac ctgggtcgtt tgatgcgtca aaaatccggt
    17941 aattacgaca aatgggataa ccgctcgaac atcagctttt ttgtcgaaca caactttgtt
    18001 ttcgataaac tcacactctc cgtaggaggg ctttataact acaacaccgc ccttgccgac
    18061 acacttaaag ccaatggttt ttatcccgaa ataaacgtat cttatcgtac aaacgatgcc
    18121 gtgagccttt acgcgtcttg gagcaaatcc acacgcatgc ctacctttac tgacttatac
    18181 tacaaggcag gaaaacaccg tggttttgct ggattaacac ccgaatactc acaatccgta
    18241 gaaattggat ggcgatacaa aaacagtgtt ttaaccgcta acctgaatgt tttttacacc
    18301 aaaggggata atcttatcga ctggatatta aaccccctgg atggatttta ccatgctcaa
    18361 aaccttgcct ccctcggcac ttacggagca ggttgggatg ccactgtaaa tatgcatcaa
    18421 tggctgggcg aacaacaacc tctgcagtcc atacaaatag gctatcaata tatgtcgcaa
    18481 aaaagcgacg aggcccgcca gaacaacccc gtttcggcgt atgtgtttaa ctatttgcgg
    18541 cacaaattta ccgttggcgt gcaacaccgg attgttaaaa atctctcgct atcttggaac
    18601 ctgcgttggc aggaccgcgc cggaaattat ttaaagtaca tcaccgagat aaaagaag
//
LOCUS       DAOSDR010000007        17638 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrimonas sp. isolate AMR_MDS_1826
            NODE_1303_length_17638_cov_4.701530, whole genome shotgun sequence.
ACCESSION   DAOSDR010000007 DAOSDR010000000
VERSION     DAOSDR010000007.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863355
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrimonas sp. (wastewater metagenome)
  ORGANISM  Petrimonas sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Dysgonomonadaceae; Petrimonas.
REFERENCE   1  (bases 1 to 17638)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17638)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:10:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,720
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 35
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17638
                     /organism="Petrimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1303_length_17638_cov_4.701530"
                     /isolate="AMR_MDS_1826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2023866"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..969)
                     /locus_tag="PLI30_00620"
     CDS             complement(<1..969)
                     /locus_tag="PLI30_00620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019538312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HOI78088.1"
                     /translation="MRQSITDTELTLETKKISVLVWEYSIPAIIGTLVNSLYNIVDRI
                     FIGQGVGAYAISGLAITFPVTILASSLGMLVGVGAASRISISLGERKKHTAEQILGNS
                     LILILLFNAVIMTLFYVYLDPILLAFGATANTLPYAREYLQIVLIGNVFISLCYSFNN
                     MMRASGYPKKAMITMLIGALLNIILDPVFIFVFDLGIAGVAWATVISMFVGMLFVMHH
                     FIQDSSLIRLRKENIRLNKNIVLAIVSIGLSPFFMQVAASGVAVLLNTSLLKHGGDLA
                     VGAYGILNSMLLIIIMTVVGLNQGTQPIIGYNYGAGNFLRVKDTFFY"
     gene            complement(1072..2355)
                     /locus_tag="PLI30_00625"
     CDS             complement(1072..2355)
                     /locus_tag="PLI30_00625"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007659941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HOI78089.1"
                     /translation="MLNPQERKKIKQLMQREIIPAIGCTEPVAVSLCVAQATETLGRK
                     PKQIEVILSSNVLKNAMGVGIPGTGMIGLPIAVALGALIGKPEYGLEVLRDLQPADVE
                     RAKQYIQDKRIKIDVEKEIEDKLYIEVRCYAGNESSVAVIRGSHTQFSYVEKNGTVLL
                     KNKTETAEEKEEDVQLTFRKVYEYAAESPIDELDFILEAARMNREASLQSQKQSYGHN
                     VAKSLTGDRGKALFGENPHSRMISATAGACDARMDGALIPVMSNSGSGNQGVAATLPV
                     LTYAEDAGCDEESLIRALILSNLSVIYIKQYLGRLSALCGCVVASAGSSCGITYLMGG
                     NYRQVTFAIKNMIANITGMICDGAKPSCALKIASGTSTAMLSALMAIENRVVTSQEGI
                     VNEDVDKTIQNLAIIGNQGMAETDRIVLQVMTHKG"
     gene            2510..3784
                     /locus_tag="PLI30_00630"
     CDS             2510..3784
                     /locus_tag="PLI30_00630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019538315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2851 family protein"
                     /protein_id="HOI78090.1"
                     /translation="MTNNEYLLHYVWKYRLFDRADLQTMDGQKVDIIDPGTHNTDAGA
                     DFFNAKIKIGDKMWAGDVEIHPSSGDWTKHGHHTDESYNSVILHVAERVKGVVRNQKG
                     VQVPQMQLIVPEDVRKNAAYLLNSKSSIPCKNHLPLVERGVICAWMVEMGLQRLERKI
                     NDIFTHMARFNNSWDHSFYVLLVRNFGFGLNSDGFERLALSLPLNCIQRHSDSLFQIE
                     ALLYGQAGLLYDDIVSDDYYLQLRKEYGFLKHKYQLKPIDGFLFKKLRVRPRAFPQVR
                     IAQLATLLKQSDRLFSSILEREDYRQLWSYFQVDTSDYWHTHYSFGKESERSRKYLGE
                     SSFHVILINTVVPMLFAYGRKTGQEKFCDRALQILETVEPERNAIVAEFAVSGISPVS
                     AFDSQALIQLKKEYCDKRKCLYCKIGNQILAR"
     gene            3978..5870
                     /locus_tag="PLI30_00635"
     CDS             3978..5870
                     /locus_tag="PLI30_00635"
                     /inference="COORDINATES: protein motif:HMM:NF024603.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing domain"
                     /protein_id="HOI78091.1"
                     /translation="MAAPTGGRYDEKPPRLIRSNPQNSALNVTRNIVEIEFDENIKVE
                     KPTEKVIITPPQINMPVIKSVGKKAVVEFNDELLPNTTYTIDFTDAIVDNNEGNPLEN
                     FSLSFSTGDQLDTLAVSGKVLSAENLEPAQGIYVGMHTNLDDTAFVRTPFERISRTDS
                     RGRFTVRGLAAGKYRIFALDDKNRDYKYDNPQEAIAFLDSIVVPSSMPAIRQDTVFKD
                     SVTIDTVKTINYTRFLPDNILLRSFSSGFQRKYFQKHERTQREKLVVFFAAPTQEPAF
                     ELLKPVSGNENWYVMEKSKDNDTLSLWISDSLVLRQDTVILKMDYLKTDSLNRDLLQS
                     DTLSFNFREPRQTRREKENEGKEEEVRLLAVNHNIQSSHEIYLPVQLEFEQPVIDFDS
                     SKVHLTYEVDSVFSHVPFTMLGDTLNPRKYTLRHKWEPGGKYKLTIDSATVHSIYGLW
                     NNKVEQTFAVKALDQYGNLMFQLSGLPVGKPAYVELLDKSDKPFRKVRVKNSEALIFD
                     INPGTLYARLFIDDNEDGEWTTGDYEKGRQPERVYYYPGAYEIRAYTDHEESWNVTEL
                     PLDKQKPLDVTKNKPEEKKRRNLNEERERQQQQQNRRGGASFPGGAGSMGGPATPMQR
                     PVAPAR"
     gene            5896..6696
                     /locus_tag="PLI30_00640"
     CDS             5896..6696
                     /locus_tag="PLI30_00640"
                     /inference="COORDINATES: protein motif:HMM:NF022750.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3108 domain-containing protein"
                     /protein_id="HOI78092.1"
                     /translation="MNKTIQLFLLFILVVSLPQGAIAQCKIINNSFKDGENISYDLYF
                     NYGIVNAKAGTGSLKTNLVNYKGNSAFNVRMLLNTSGLAGSVYTVNDTLVSYIDMNLR
                     PLLFTKNAFEGKDYSREVQSFSYVEDGIKVRTNRVFNGKKKFEETITTHECTYDYLSV
                     LALIRNLDYTDMEPGDQKQIQFISGREIVNMAVNYNGLSKIKANDGQTYNTVQISMTI
                     YDKAFKDKKEAISASLTDDANRIPIIINTHLKIGAIRAVLKNVSGLRN"
     gene            6816..7226
                     /locus_tag="PLI30_00645"
     CDS             6816..7226
                     /locus_tag="PLI30_00645"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative quinol monooxygenase"
                     /protein_id="HOI78093.1"
                     /translation="MQKQFFLFCVLFLTLLSAYPRHSQAQNAGEPYVILVEFTLDKSR
                     VDEAIELLSDIQLQTLENEEGCLVYDVLLSEEDPARVFIYESYESEAAYKVHSNASYF
                     KEIVLKKLKPLIKSQKITKVFPLNFEGVLGDAEI"
     gene            complement(7233..7820)
                     /locus_tag="PLI30_00650"
     CDS             complement(7233..7820)
                     /locus_tag="PLI30_00650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018335961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase family protein"
                     /protein_id="HOI78094.1"
                     /translation="MMLPIETHPLQPFLPGKTRLLMLGSFPPPKAKWKMDFYYPNFQN
                     DMWRILGLAFFNEKDYFLTENKSSFDKEKIMEFLSLKGIAVCDTAHEVHRLKGNASDN
                     FLEIVTPLNLENILSKIPDCKAFATTGEKATETLRSLFPATTPIPKIGNSVTVDFLNK
                     KYKFYRMPSSSRAYPRPLSEKAKIYRQLFTEMEIL"
     gene            complement(7821..9386)
                     /locus_tag="PLI30_00655"
     CDS             complement(7821..9386)
                     /locus_tag="PLI30_00655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009040554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HOI78095.1"
                     /translation="MELKQNNLLFTIAGTALLASCTGTEKTVNTKPLNIVYIMTDDHT
                     RQMMSCYDKRHIETPNLDRIANNGVRFTNAFVANSISAPSRACLLTGKHSHKNGKIDN
                     ITSFDGSQQTVQQLLQQAGYQTAMIGKWHLDSQPTHFDYWTILPGQGHYYQPDFITPE
                     GRKNHQGYATNLITDFGLDWLENGRDKKKPFCLFLHHKAAHRNWMSDTTYLKAYEDKT
                     FELPTNFYDDYSGRKAAKMQEMSIASDRDMDIVNDLKMLRPGVSTRLSEQYIQGEYAR
                     LDSAQKTKWDAHYQPIIDAFYASGLQGNELAEWKYQRYMRDYAKTIKSLDENVGRILD
                     YLEKNGMLENTVVIYTSDQGFYMGEHGWFDKRFMYEESFSTPLVMWLPKSYKKRGDIP
                     QLVQNIDFAPTMLELAGAPIPSDIQGVSLVPLLKNEKAPANWRKSLYYHYYEYPGEHA
                     VRRHYGVRTERYKLIRFYGDIDEWELFDLQADPMEMNNLYGKTGYEKITGELCAELKR
                     LQEQFDDPIRKDN"
     gene            9513..10160
                     /locus_tag="PLI30_00660"
     CDS             9513..10160
                     /locus_tag="PLI30_00660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005791743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HOI78096.1"
                     /translation="MSMNHDNYHSRIPEPALRRLPWYLSYVKLLKKQGYSKVSSTQIA
                     KKINVSATQIAKDLSYVNVSGKTRVGYDIDKLIGVLEDFLGFKKMHTAVLFGVGSLGA
                     ALLSDSGLEQYGLKIRAGFDVNPSIVGQKINNIPVFHSDDFAAKNKKIDAQIGILTVP
                     PDIAQEVADFMIAGGIQAIWNFTPFRIRVPEHIVLQNTSLYAHLAVMFNRLVKEG"
     gene            10166..10780
                     /locus_tag="PLI30_00665"
     CDS             10166..10780
                     /locus_tag="PLI30_00665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010536512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HOI78097.1"
                     /translation="MKIFAVGLNYDSHNREMKRVFEASEPVIFMKPDTALLRNGNPFF
                     LPDFSSEIHYETELVLKINRLGKNIAEKFAHRYYDEITVGIDFTARDLQRKQKELGLP
                     WEIAKGFDNSAPVGEFISIKKIPDINNINFRLDINEKTVQQGNSCDLIYSFDKIIAYI
                     SRFFTLKIGDLVFTGTPAGIGGVSINDRLEGYIENKKLLDFKIK"
     gene            10840..12009
                     /gene="porV"
                     /locus_tag="PLI30_00670"
     CDS             10840..12009
                     /gene="porV"
                     /locus_tag="PLI30_00670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008862485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IX secretion system outer membrane channel
                     protein PorV"
                     /protein_id="HOI78098.1"
                     /translation="MQSKSSFFTTVCFLSLFILFSDKAFAQQYNPIPVAMPSLQIAPD
                     ARGGGMGDIGAASMPDVYSQYWNAAKYPFISGSTGFSISYTPWLSKLVNDIDLVYLTG
                     FWKFGNENLNAISTSLRYFSLGDVDVFDQNTDYVMAVSPHEFAFDVAYSRKLSETFSG
                     AVTLRYIRADYSTGSDDVTPGNAFSADISGYNETYLNLGQSEALLGFGFNISNIGTKI
                     SYDGGNTSMFLPANLRVGTSLGLPVDDKNSFSFSVDVNKLLVPTPQLPAEGESEEERT
                     KRVQKYADTSPVAGIFNSFGDAPGGFKEEMQEVIWSLGVEYSYDNRFSLRTGYYHESE
                     YKGNRRYMAFGAGFKMNAFQIDAAYLVSTAQSNPLDQTLRLSLGFDIDGIKQLLR"
     gene            12025..12504
                     /gene="ispF"
                     /locus_tag="PLI30_00675"
     CDS             12025..12504
                     /gene="ispF"
                     /locus_tag="PLI30_00675"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009231428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HOI78099.1"
                     /translation="MKIRVGFGYDMHRLAEGRDLWMGGIKIDFEKGLQGHSDADVLIH
                     SICDALLGAANLRDIGFHFPDTADEFLGVDSKILLKKSVALLRENGFEVGNIDATICA
                     EAPKMNPHVPAMQKTLSEIMETDVNTISIKATTSEKMGFVGRQEGITAYAVALIYTL"
     gene            12517..13143
                     /locus_tag="PLI30_00680"
     CDS             12517..13143
                     /locus_tag="PLI30_00680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI78100.1"
                     /translation="MCFKWFKRRKQARGFGIHSPFAYNFVTHIINEEYGYTAFLDIDQ
                     LLRAHNIETSDKKLHHLSYKLVRYFKPGRVLELDTGKGINTLYLSAASKDAVCHCFDP
                     EGRNVLLARNLHTTYNRHVEFIDEIDLSELYDGIFVYLNSFPVDMESLLSMSSDDTFW
                     VIAGIKSGNGKRFWKAVVADERARITFDMKDIGVVVLKKSYHKASYLI"
     gene            13227..13775
                     /locus_tag="PLI30_00685"
     CDS             13227..13775
                     /locus_tag="PLI30_00685"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008772935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HOI78101.1"
                     /translation="MKKSTVYTKSGDKGKTSLVGGTRVKKTHVRLGAYGTIDELNSFI
                     GWLNCGVDDEETGLFLSFLQHKLFTVGSYLATETEQIPPKAASIISPEDIEKVEKEID
                     RIDHTLPRLNRFILPGGNEAAARAHICRTVSRRAERNVYRVAEEFPVADFVLIFLNRL
                     SDYFFVLARYESNKTGKEIYWE"
     gene            13845..14066
                     /locus_tag="PLI30_00690"
     CDS             13845..14066
                     /locus_tag="PLI30_00690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004362633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2795 domain-containing protein"
                     /protein_id="HOI78102.1"
                     /translation="MYWTLELASKLEDAPWPATKDELIDFAQRSGAPLEVIENLQEIE
                     DDTEVFETIEDIWLDYPSKEDFFFNEDEY"
     gene            complement(14133..14945)
                     /gene="pgeF"
                     /locus_tag="PLI30_00695"
     CDS             complement(14133..14945)
                     /gene="pgeF"
                     /locus_tag="PLI30_00695"
                     /inference="COORDINATES: protein motif:HMM:NF014619.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan editing factor PgeF"
                     /protein_id="HOI78103.1"
                     /translation="MFRHPNYSNLLFFTIFSNEQRLLHFSTTRAGGVSRGEFRSLNLG
                     NYSDDNPLNIFENRSIVARKFYKEANDLITPHQTHGNRVLLIDAEFLNLSNAEKIERL
                     YGYDASVTQEKGYFLCVTTADCVPLLLFDRKNEAIGAIHAGWKGTSGRIVEKTLLEMK
                     KNFGTETRDILAAIGPAIGIDNYEVGREVEEEFQKSGFNLNKSISYRHNLSRKLHIDL
                     KEINRQELIRMGVPKHQIEKTRYCTYGNSRLFFSARRQSLHSGRMLSGIMLR"
     gene            complement(14949..16091)
                     /gene="obgE"
                     /locus_tag="PLI30_00700"
     CDS             complement(14949..16091)
                     /gene="obgE"
                     /locus_tag="PLI30_00700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019541208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HOI78104.1"
                     /translation="MAETNFVDYVKIFCRSGKGGRGSTHFRREKYIPKGGPDGGNGGD
                     GGHIILRGNRNYWTLLHLKFQRHIFAGHGESGSRRNSSGKRGDSKIIEVPCGTVAYDA
                     HTGEYLCDITDDGQEIVLLKGGRGGLGNTNFKSATNQAPRYSQPGEPYEEKWIILELK
                     LLADVGLVGFPNAGKSTLLSVVSAAKPKIGNYPFTTLEPNLGIVSYREGKSFVMADIP
                     GIIEGASEGKGLGLRFLRHIERNSLLLFVVPADAENIHEEYNILLNELTKYNPELLDK
                     HRVLAISKADMLDDELIHEISYDLPKIPYVFISSITGYGIPALKDILWRELNSEETIH
                     VSTSGESLVHRRLDVQSLEFEPEEEIEEDLDDEYGEEEENYYEEDF"
     gene            complement(16093..16671)
                     /locus_tag="PLI30_00705"
     CDS             complement(16093..16671)
                     /locus_tag="PLI30_00705"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005854805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019205 - nucleobase-containing compound
                     kinase activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HOI78105.1"
                     /translation="MLNIVIFGAPGSGKGTQSDLLIKKYGLKHVSTGDILRFEIKAGS
                     ALGKIADQYISKGHLVPDEIMIEMLEDLIRKNLDKKGFIFDGFPRTLPQGEALDNKMK
                     KNGLDITSVLSLEVKDEELIDRLLKRGQLSGRSDDNGHTIKSRLNVYHQQTEPLKEYY
                     AGQNKLVCIPGEGAIEEIFNSIVEVVDNLTGR"
     gene            16939..17295
                     /locus_tag="PLI30_00710"
     CDS             16939..17295
                     /locus_tag="PLI30_00710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI78106.1"
                     /translation="MNKQEFKQKTQEILDQLDAKIDEMKQGISNITEDAKEEYAEQIE
                     KLKSLKDELSEKLGKFDDIAENKWDVVRESASNFFSKVGEAWKEDFERVKQAFKKETD
                     TASGMAQDTETGAKED"
     gene            complement(17350..>17638)
                     /locus_tag="PLI30_00715"
     CDS             complement(17350..>17638)
                     /locus_tag="PLI30_00715"
                     /inference="COORDINATES: protein motif:HMM:NF015871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HOI78107.1"
                     /translation="APTSSETVKADQQKQLDQINERYQMFLQNSQREMEELRQKLLAP
                     VQQKIATAIKAVGDEKGYTYIFDLAAGNPVYFNATNAEDATPLVKTKLGIK"
BASE COUNT         4865 a         3733 c         3978 g         5062 t
ORIGIN      
        1 atagaaaaaa gtgtctttga cccgcaaaaa gttccctgca ccgtagttat acccgataat
       61 cggctgcgta ccctggttta accccactac agtcataatt atgatcaaca acatggagtt
      121 aagtattcca taggcaccta cggctaaatc gcctccgtgt ttcaacagtg aggtgtttaa
      181 caaaacggct actcctgatg ctgctacctg cataaaaaag ggagaaagac cgatagatac
      241 gatagccagg acgatatttt tatttagccg gatattctct ttccgcaagc gtataagact
      301 gctgtcctgt atgaaatgat gcatcacaaa taacattcct acaaacatgg aaataactgt
      361 ggcccaagcc actccggcaa taccgagatc aaaaacgaaa ataaacaccg ggtcgaggat
      421 gatgttcaac agagctccga ttaacatcgt tatcatcgct tttttgggat atcccgaggc
      481 ccgcatcatg ttattaaagc tgtaacagag actgataaag acatttccga taagaacgat
      541 ttgtaaatat tcccgtgcgt agggcagtgt gtttgcggta gctccgaaag cgagaaggat
      601 aggatcgaga taaacgtaga aaagcgtcat gatcactgcg ttgaacaaaa gtataagtat
      661 cagggagttg ccgaggattt gttctgccgt atgtttcttc ctttcgccca aactgataga
      721 aatccggctt gcggcaccta caccaaccaa catcccgagc gaagaggcaa gtatggtaac
      781 cgggaaagtg atggccagtc ccgaaatagc atacgctcca accccttggc ctataaaaat
      841 tcggtcaacg atgttgtaga gcgaatttac aagggtgccg atgatggcgg gaatagaata
      901 ttcccatacc agaacgctta ttttttttgt ttcgagagta agttccgtat ctgtgatcga
      961 ttgtcgcaca gtgaaattgc gctttaaaat tttaattttc cggtaactac cggcacagca
     1021 agatctgtcc gtcttataac gaaatttcca gggtttatgt tcgaacgatt tttatccttt
     1081 gtgcgtcatt acttgcaata caatcctgtc ggtttctgcc atgccctggt taccaatgat
     1141 ggctaggttt tgaatggtct tgtccacgtc ttcattcacg ataccttcct gtgacgttac
     1201 cacccggttt tctatggcca tcagggcaga gagcattgcc gtggaggtgc cgctggctat
     1261 tttaagtgca caactgggct ttgccccgtc gcaaatcata ccggttatgt tggcaatcat
     1321 gttttttatg gcgaacgtaa cctgtctata attgcctccc ataaggtacg ttatgccgca
     1381 gctggaaccc gctgacgcca ctacgcaacc gcataatgcc gataaccttc ccaggtattg
     1441 tttgatgtaa ataaccgata ggttgctgag aatcagtgct ctgataagac tttcttcgtc
     1501 acagccagcg tcctctgcat aggttaacac gggaagtgtc gcggctacac cctggtttcc
     1561 gcttccggag ttgctcatca ccggaattag ggctccgtcc atccttgcat cgcaggcacc
     1621 agccgttgca gagatcatgc ggctgtgtgg attttctccg aaaagggcct ttcccctatc
     1681 gcctgtcagg cttttggcca cgttgtgtcc gtaactttgt ttttgggatt gtagtgaagc
     1741 ttcccgattc attcttgccg cttcaaggat gaaatccaat tcatctatag gcgattcagc
     1801 ggcatattcg tatactttcc ggaacgtgag ctgtacatcc tcttcctttt cttctgctgt
     1861 ttccgtttta tttttcagca gcacagttcc gtttttttcg acgtaggaga actgggtatg
     1921 gctgccgcga ataacagcta cggacgattc atttccggcg tagcaccgga cttctatata
     1981 cagtttatct tcaatttcct tttcaacatc gatttttatt cgtttgtctt ggatgtattg
     2041 tttggcgcgt tccacgtccg caggttggag atcccggaga acttccaggc cgtactccgg
     2101 tttcccgatc agtgcgccca aggcaacggc tataggcaac ccaatcattc ctgttccggg
     2161 aattcctaca cccatagcgt ttttaaggac gttggaactc agaattacct cgatctgttt
     2221 gggttttcgt cccagtgttt ccgttgcctg tgcgacacaa agcgatacgg cgacaggctc
     2281 tgtgcaaccg atagccggaa ttatttcacg ttgcatcagt tgcttgattt tttttctctc
     2341 ttgcgggttt aacatgaaaa tttgtggttc aatgatttta agtatacggc aaagttacat
     2401 aaaaatcgac agaaagccgg ttgaaaggag aaataatgaa aaacagacgt accgttgaca
     2461 ggattaaaat ataattgtat atttgaactc caaacagcct taaagagata tgacaaacaa
     2521 cgaatatttg cttcattatg tttggaaata ccggcttttc gaccgggctg acctccaaac
     2581 gatggatgga caaaaagtag atatcatcga tccgggaaca cacaacactg atgccggagc
     2641 tgatttcttc aatgccaaaa tcaaaatagg ggataagatg tgggcagggg atgtggagat
     2701 tcatccatca tcaggtgatt ggaccaaaca cggacaccat accgatgaaa gctacaactc
     2761 ggtgattttg cacgtggcgg aaagagtgaa aggtgtggtg aggaaccaaa aaggtgtgca
     2821 ggttccgcaa atgcaattga ttgtacctga agatgtaaga aaaaatgcag cgtatttgct
     2881 caacagcaag tcgagtatcc cttgcaagaa tcatctcccg ctggtggaaa ggggagtaat
     2941 atgtgcctgg atggttgaga tggggcttca acggttggag cggaagatca acgatatttt
     3001 tacacatatg gcccgattca acaattcatg ggatcattcc ttttatgtgt tgcttgtccg
     3061 gaactttgga ttcggattaa attccgacgg atttgaacga ctggcgttga gccttcctct
     3121 caactgtatt cagagacaca gcgatagcct ttttcagata gaagcgttgc tgtatgggca
     3181 agccggattg ttgtatgacg acattgtttc cgatgattac tacctgcaac tcagaaaaga
     3241 atacgggttc ttaaaacaca aataccaact taagcctatc gatggatttt tattcaagaa
     3301 gttgcgtgtc cgtccccgcg catttccaca agtacgtatc gcacagctgg caactctgct
     3361 gaagcagtcg gaccgtctct tttcttccat actcgagaga gaagactata gacagttatg
     3421 gagttatttt caggttgata cctccgatta ctggcatacc cattattctt tcggaaaaga
     3481 gtcggaaaga tcccgaaagt acctggggga atcttctttc catgttatcc ttatcaacac
     3541 ggttgttcct atgctctttg cttacggaag aaaaaccggc caggagaaat tttgtgacag
     3601 agcgttgcag atactggaga cggtagaacc agaacgaaat gctatcgttg ctgaatttgc
     3661 agtttccggt atatctcctg tcagtgcatt cgattcgcaa gcgctcattc aattgaaaaa
     3721 agagtactgc gataagcgga agtgcctgta ctgcaaaatc ggaaatcaaa tcttagcccg
     3781 atagcgctgc aaaacatctt gtatttactg aatttccgtt tttttaaaca tcaaaacgct
     3841 aaaaactact taattcgttg tatttttgcg ccaattatgc aaatgacagg aaggtaataa
     3901 tgataaggtt gttgaaatat atactcatac tcatttttcc gggaggaata ctgttcgtat
     3961 tgaattcatg cgccaatatg gcagcgccca cgggtgggcg gtacgacgaa aagccacccc
     4021 ggctgatccg ttccaatcct caaaacagcg cattaaacgt aacccgcaac atcgttgaaa
     4081 ttgaatttga cgagaacata aaagttgaaa aaccgacaga gaaagtgatc attacgcctc
     4141 cgcagataaa tatgcctgtg ataaaatcgg tgggaaagaa agccgttgtg gaattcaacg
     4201 atgaactatt gccaaacact acttatacga tcgattttac tgatgctatt gtcgataata
     4261 acgaagggaa tccgctcgaa aacttctccc tctctttctc tacaggagat caactcgata
     4321 cgctcgccgt ttccggcaaa gtgttatccg cagaaaacct ggaacctgct caaggtattt
     4381 atgtgggcat gcacaccaac ctggatgaca ccgcttttgt aaggactcct tttgaaagga
     4441 tttcaaggac tgactcacgc ggaaggttta cagtaagagg attagccgca ggtaaataca
     4501 ggatttttgc tctcgacgat aaaaacaggg attataaata tgacaacccg caggaggcca
     4561 tagcctttct tgattccatt gtcgtgccgt catcgatgcc tgctatacga caggatacgg
     4621 ttttcaaaga cagtgtgacc attgataccg taaaaacgat aaattacacc cgttttctgc
     4681 ctgataatat tttacttcgc tcattttcat ccggatttca acggaaatat tttcaaaaac
     4741 acgaacgaac ccagcgtgaa aaacttgttg tcttttttgc ggctccaacc caggagcctg
     4801 ctttcgaact gttaaaaccg gtatcaggca atgaaaactg gtacgtgatg gagaaaagca
     4861 aagataacga taccctttcc ctgtggatat ccgactcatt ggtcttaagg caggataccg
     4921 ttatactgaa gatggattac ctgaaaacag attcgttgaa ccgggacctt ttgcagtcgg
     4981 atacattgag ttttaacttc cgcgaaccgc gacaaacccg tagagaaaaa gaaaacgaag
     5041 ggaaggaaga agaagtgcgt ttgcttgccg ttaatcacaa tatccaatct tcacacgaaa
     5101 tttacttgcc ggttcagttg gagtttgaac aaccggtaat cgattttgat tcatcgaaag
     5161 ttcacctcac atacgaagtg gactctgttt tctcgcatgt ccctttcact atgcttggcg
     5221 acacgctcaa cccccgaaag tatacgcttc ggcacaaatg ggaacccgga gggaagtata
     5281 aattaaccat cgattcagcg actgttcaca gtatttacgg attgtggaac aacaaggtag
     5341 agcagacgtt tgccgtaaaa gcactggatc agtacggaaa cttaatgttt cagctgtcgg
     5401 gactgccggt agggaaaccg gcttatgtag agttgctgga caaatcggat aaaccttttc
     5461 gaaaagtacg tgtcaaaaac agcgaagccc tgattttcga tattaatccg ggaacgctgt
     5521 atgcccgact tttcatcgat gacaacgaag acggtgaatg gacgacaggt gactatgaga
     5581 agggtagaca gcccgaaaga gtttactatt atccgggtgc gtatgagatt agggcataca
     5641 ctgaccatga agagtcgtgg aatgtgacgg agttgccgtt agacaagcaa aaaccgcttg
     5701 atgtcactaa aaataaaccc gaagaaaaga aacgccgtaa cctcaacgaa gaacgcgaac
     5761 gccagcaaca gcagcaaaac cgacgcggag gagcttcttt tcccggcggg gccggaagta
     5821 tgggcggacc ggccactcca atgcagagac cggttgctcc tgcccgataa ccgcacttaa
     5881 tccaaacgaa cgaaaatgaa caaaacgatt caattgtttc tcttatttat cttggttgtc
     5941 tccctgcctc agggagcaat tgcccagtgt aagatcatca ataactcttt taaagacgga
     6001 gagaacatct cttacgattt gtatttcaat tacggcatag tgaatgccaa agccggcaca
     6061 ggatcattaa agaccaatct ggtaaactac aagggcaaca gcgctttcaa tgttcgcatg
     6121 ttgcttaata cgagtggatt ggcaggaagt gtttatacgg ttaatgatac gcttgtgtcg
     6181 tatatcgaca tgaatttgcg accgctgctc ttcactaaaa atgcatttga aggaaaggat
     6241 tattcccggg aagtgcagtc tttcagttat gtagaggatg gaataaaagt aaggacaaac
     6301 cgtgttttta acggaaaaaa gaaatttgag gaaaccatta ctacccatga atgtacatac
     6361 gattacctgt cggtattggc gttgatccgg aacctggact acacggatat ggaacccggt
     6421 gaccaaaagc aaatacagtt tatttccgga agagagatcg tgaacatggc tgtaaactac
     6481 aacggtttgt ccaagattaa agccaatgac gggcaaacct acaacacggt tcaaatctcc
     6541 atgactatct acgataaggc gtttaaagac aagaaagagg ctattagtgc ttcattgacc
     6601 gatgatgcga acagaattcc cattatcatc aacacgcatt taaagattgg agctatacgc
     6661 gctgtgctga aaaatgtttc cggtctgcga aattaagaaa ctgttttgaa gtttcctttt
     6721 gttccatacg ccgtatgatg cgaaaatcca ggttcataaa aaatgacaca aacagtgcat
     6781 aaataatcaa aacagttttt aaattttctt aacctatgca aaagcaattt tttcttttct
     6841 gtgtgctttt tctgacgctt ctttctgctt atcctcgcca ttcacaggca caaaatgcag
     6901 gtgaacccta tgtgattttg gttgaattta cgttggataa gagccgggta gatgaggcca
     6961 tagagttgtt atcagacatt caattgcaaa cccttgaaaa cgaagagggt tgcctggttt
     7021 acgatgtttt gttgagtgaa gaggatcctg ctcgtgtatt tatctacgag agctacgaga
     7081 gtgaagctgc atataaagtg cattccaatg cttcttattt taaagagatt gttctgaaaa
     7141 aactgaagcc actcatcaaa agccaaaaga taaccaaggt atttccattg aatttcgaag
     7201 gagtgttagg tgacgctgag atttaacgct ctctaaagaa tttccatctc tgtaaaaagt
     7261 tgcctgtata ttttggcctt ttcggataat ggcctggggt atgctcttga tgaggaaggc
     7321 atccggtaaa atttgtattt cttattgagg aaatcaacgg tcacgctgtt cccaatctta
     7381 ggaatgggag ttgtggcggg aaaaagcgac cgaagagtct cagtcgcttt ttcaccggtt
     7441 gtagcgaacg ctttgcagtc agggattttc gataaaatgt tttcaaggtt gagcggagta
     7501 actatttcca gaaaattatc cgacgcattt cctttcagcc ggtgcacttc gtgtgccgta
     7561 tcacatacgg caataccttt caaggatagg aattccataa ttttttcttt atcgaaagag
     7621 gacttgtttt ccgtcagaaa ataatccttt tcattgaaaa acgccagccc caaaatccgc
     7681 cacatatcgt tttggaaatt gggataatag aaatccatct tccacttcgc cttcggtgga
     7741 ggaaagctgc ccagcatcag taacctggtt ttgccgggta aaaagggttg caaagggtga
     7801 gtctcgattg gaagcatcat tcagttgtcc ttcctgatcg ggtcatcgaa ttgctcctgg
     7861 aggcgtttaa gttcggcgca aagctcgccg gtaatttttt cgtatccggt ttttccgtac
     7921 aggttgttca tttccatcgg atcggcttgc aggtcgaaca gttcccactc atctatgtca
     7981 ccgtagaaac gaatgagttt gtaccgttcc gttctcactc cataatgacg ccttacggca
     8041 tgttcaccgg gatattcgta gtaatgataa tacagggatt ttctccaatt ggcgggagct
     8101 ttttcgtttt tgagtaaagg taccagggag acgccttgta tatctgaggg gatgggagca
     8161 cccgcaagct caagcattgt cggtgcaaaa tcgatgtttt gaaccagttg tggtatgtcg
     8221 ccccgtttct tgtatgattt gggaagccac ataaccaggg gtgtgctgaa cgattcttcg
     8281 tacatgaatc gtttgtcgaa ccaaccgtgt tcacccatgt aaaaaccctg atcggacgtg
     8341 taaatgacaa cggtgttctc cagcattccg tttttttcca ggtagtcgag aatacgcccc
     8401 acattttcat ccagcgactt tatggttttg gcgtaatcac gcatatagcg ttgatatttc
     8461 cactctgcca attcgttgcc ttgtaagcca gacgcgtaaa atgcatcaat gattggctgg
     8521 taatgagcat cccattttgt tttctgtgca gaatccaacc gggcatattc cccctggata
     8581 tattgttccg ataaccgggt gctgacaccc ggacgcagca tcttcaggtc attcacaatg
     8641 tccatatcgc ggtcgctagc gatgctcatc tcctgcattt ttgctgcttt tctccctgaa
     8701 taatcgtcgt aaaaattggt agggagctca aatgttttgt cttcgtaagc ttttaggtaa
     8761 gtcgtatcgg acatccagtt acggtgtgcc gctttgtgat gcaggaacag gcagaaaggc
     8821 tttttcttgt cgcgtccgtt ctcgagccaa tccagtccga agtcagtaat gaggtttgtg
     8881 gcgtacccct ggtggttttt tcttccttca ggggtgataa aatcgggttg ataataatgg
     8941 ccttgaccgg gaagaattgt ccagtagtcg aaatgagtgg gttgactgtc caggtgccat
     9001 tttccaatca ttgctgtttg gtatcccgcc tgttgcagca gttgctggac ggtttgctga
     9061 ctgccgtcga acgaagtgat gttgtcgatt tttccgtttt tgtggctgtg ctttccggtc
     9121 agcagacatg cacggctggg agcagagatg gagtttgcca cgaaagcatt ggtaaatctc
     9181 actccgttat tagctattct gtcgaggtta ggtgtttcta tgtggcgctt gtcataacaa
     9241 ctcatcattt gccgggtatg gtcgtccgtc atgatataaa cgatgttcaa cggtttcgta
     9301 tttaccgttt tttctgttcc tgtgcacgaa gccagcaagg ctgttccggc gatggtgaat
     9361 aatagattgt tctgtttgag ttccattggc tcttgggatt ccgttttcac aaaaatagtt
     9421 ttttttgtcc aattacggac ttttttcttt aattatctgc cgggataaaa atttttagca
     9481 aaaaacgagt atctttgcaa attaaaacag atctgtcaat gaatcacgac aactatcatt
     9541 cgagaatccc tgaaccggct ttgcgaaggc tgccgtggta tttgtcttac gttaagttgt
     9601 taaaaaaaca aggatacagc aaagtgtctt ctacacaaat cgccaaaaag atcaatgtgt
     9661 cagccactca aattgcgaaa gatctttcct atgtaaatgt cagcggtaaa acccgggtag
     9721 gctatgatat tgataagctg ataggtgtgc tggaagattt tttgggtttc aaaaagatgc
     9781 ataccgccgt gcttttcggg gtaggaagtc tgggggctgc cttgctttcc gattcgggtt
     9841 tagagcaata cggcctaaag atacgtgctg gatttgacgt gaatccatcc atcgtcggac
     9901 agaaaattaa caacatcccc gtgtttcact ccgatgattt cgcggcaaaa aacaaaaaaa
     9961 tagatgccca aattggtatt ctcaccgttc cccccgatat tgctcaggaa gttgccgact
    10021 ttatgattgc gggagggatt caggcaatat ggaattttac tcctttccgg attcgcgttc
    10081 ccgagcacat tgtgttgcag aatacatcgc tttacgcgca cctggcggtc atgtttaacc
    10141 ggcttgtgaa ggaggggtag caccaatgaa aatttttgct gtcggcttaa attacgattc
    10201 gcataaccgg gagatgaagc gggtctttga ggccagtgaa ccggttatct ttatgaaacc
    10261 cgatacggct ttattgcgta acggaaaccc ttttttcctg cctgattttt ccagtgaaat
    10321 tcattacgaa acagaattgg tgctaaaaat taaccggttg ggaaagaaca ttgccgaaaa
    10381 atttgctcac cgttattacg atgaaattac cgtaggtata gattttaccg cccgtgattt
    10441 gcaacggaaa caaaaggaat tgggcctgcc ttgggagata gccaaaggct ttgataattc
    10501 tgcgcccgta ggagaattta tttccataaa gaaaataccg gacattaaca acataaactt
    10561 ccgtctcgat atcaatgaaa aaactgtaca gcaagggaat agttgtgatt tgatttactc
    10621 tttcgataaa atcatcgctt acatcagtcg cttttttacg cttaagatag gggatcttgt
    10681 ctttaccgga actccggcag gaattggagg tgtatccatc aacgatcgtc ttgagggcta
    10741 tattgaaaat aagaagctgc tcgattttaa aattaaatag caccaataca ataaaaagag
    10801 attatatttg tttagaaatt aaacaacata tacagatcta tgcaatcgaa atcaagtttc
    10861 ttcactaccg tttgtttctt gagcctattt atactgtttt ccgacaaggc ttttgcccaa
    10921 caatataatc cgattcccgt ggcaatgcct tctcttcaga tcgcacccga tgcccggggc
    10981 ggcggtatgg gcgatatagg tgctgcctcc atgcccgatg tctattcaca gtattggaat
    11041 gctgccaagt atccgtttat atccggcagt accggatttt cgatctcgta tactccctgg
    11101 ttaagtaagc tggtaaacga cattgatctg gtgtatctga ccggtttttg gaaattcggg
    11161 aacgaaaacc tgaacgctat cagtacttca ctccgttatt tttctctggg agacgttgat
    11221 gtatttgatc agaacaccga ttacgtgatg gccgtatccc cccatgaatt tgctttcgat
    11281 gtggcgtatt caagaaaact ttccgaaacc ttttcaggag cggtcacctt gcggtatata
    11341 cgtgccgatt attccacggg aagcgatgac gtgacacccg gcaacgcttt ctcagccgat
    11401 atttccggtt acaatgagac ctatctcaac ctgggacagt cggaagcatt gttggggttt
    11461 gggtttaata tttcaaatat aggaacaaag atatcttatg acggaggcaa cacatccatg
    11521 tttttgcctg cgaacttgag ggtgggtaca tcactggggc tgccggttga tgataaaaac
    11581 agtttctctt tcagtgttga tgtcaacaag ttgcttgttc cgacgccaca actgcctgct
    11641 gaaggggaga gcgaagagga gaggacgaaa agggtacaga aatatgccga tacttctccc
    11701 gttgccggta ttttcaactc ttttggtgat gctcccgggg gatttaagga agagatgcag
    11761 gaagtgatct ggtcgctggg agtagaatat tcttacgata atcggttttc gctccgtacg
    11821 gggtattatc acgagagcga atacaaggga aatcgccgtt atatggcttt tggagccgga
    11881 tttaaaatga acgcctttca aattgatgcg gcttatttag tctcaacggc ccagtccaat
    11941 ccgttggatc agaccttgcg cttgtcgctc ggttttgata tcgatggaat aaaacagttg
    12001 ttgcggtaat cgagtaattt ttctatgaag ataagggtag gttttggata cgacatgcat
    12061 cgcctggcag aaggcagaga cctctggatg ggaggaataa aaatagactt tgaaaaaggc
    12121 ctgcagggtc attccgatgc cgatgtattg atccacagca tttgtgatgc attgcttggt
    12181 gcggccaacc tgcgggatat cggttttcat tttcctgaca ccgcagacga gtttctgggt
    12241 gtcgacagta aaatactgtt gaagaaaagc gttgccttgt tgcgagaaaa tgggtttgag
    12301 gtaggtaaca ttgacgcaac catctgtgcc gaagctccca aaatgaaccc gcatgttccg
    12361 gctatgcaaa agacgttaag cgaaataatg gagacggatg tcaacactat ttccatcaag
    12421 gccaccacct ctgaaaagat ggggtttgtg ggccggcagg aaggcataac agcctatgca
    12481 gtagctttaa tttatacatt ataattgttc ccggttttgt gtttcaagtg gtttaaacga
    12541 agaaagcagg caagagggtt cggtattcat tctccttttg cttataattt tgtcacccat
    12601 attattaacg aagaatatgg gtacacggca tttctggata ttgaccagct gttacgcgca
    12661 cacaacatag agacgtccga taaaaagctt catcatttgt catacaagct tgtcaggtat
    12721 tttaaacccg ggagggttct cgaactggat acgggaaagg ggattaatac gctctatcta
    12781 agtgcagctt ctaaggatgc cgtttgtcac tgttttgatc cggaagggag aaatgttttg
    12841 ctggcaagaa atttgcatac aacgtataac aggcatgttg agtttatcga tgaaatagac
    12901 ctttccgagt tgtacgacgg aatatttgtc tatttaaact catttcctgt tgatatggag
    12961 agcctgctat cgatgagttc agacgatacg ttctgggtta tcgcgggaat aaaatcgggt
    13021 aatggcaaac gtttctggaa ggctgtcgtc gcagatgaaa gggcaagaat tactttcgac
    13081 atgaaagata ttggtgttgt cgttttaaag aaatcgtatc acaaagcgag ttatttgatt
    13141 taaaaaggca attagacatt aaataagagg taaaattcct gacaataaaa caacctgagt
    13201 gcctgaggcc taaaatggat tttgaaatga aaaaaagtac tgtatataca aaatcgggag
    13261 ataagggtaa aacatcgttg gttggcggca cccgggtaaa aaaaacgcat gtccggctag
    13321 gggcttacgg aacaatcgac gaattgaaca gttttatcgg ttggttgaac tgcggtgttg
    13381 atgatgagga aaccggtctc ttcctttctt ttctacaaca caagcttttt acggtaggct
    13441 cttacctggc aacagaaaca gagcagattc cccctaaagc tgccagtatc atctccccgg
    13501 aagacattga aaaagttgaa aaggaaatcg atcggataga ccatacttta ccccggttga
    13561 accgttttat tcttcccgga ggaaatgaag ctgctgcacg tgcacatatc tgtcgcaccg
    13621 tttcccgaag agctgaacga aacgtttatc gggtggctga agaatttcca gtagccgatt
    13681 ttgttctaat tttcttaaat cgtttgtcag actacttttt cgtgctcgca cgatacgaaa
    13741 gtaacaagac cggaaaagaa atatactggg aataggataa tatctgattt ttttgtatat
    13801 ttttgcgcta aatttatgaa acaacaaacc ctaaggagat aattatgtat tggacactcg
    13861 aattagcttc taaattggaa gacgctccct ggccggcaac aaaagacgag ctcattgatt
    13921 ttgctcaacg ttccggtgcc ccgcttgaag tgattgagaa tttacaggaa attgaggacg
    13981 acacggaagt ttttgaaacg atagaagata tatggctcga ttatcccagt aaagaagact
    14041 tcttttttaa cgaagatgag tattaatgtt ttccaaaaaa acaaaatctt acagccgtcc
    14101 gactttcctg gcggctgttt tgttggtagc cgttaacgaa gcattatacc ggaaagcatt
    14161 cttccagagt gcaacgattg ccgccgggcc gagaaaaaca ggcggctatt accgtaggta
    14221 cagtaccgcg ttttctcaat ctggtgcttg ggaacgccca tcctaatgag ttcctgtcgg
    14281 ttgatttcct tgagatcaat atgcaacttc cgggataagt tatggcgata cgaaatggat
    14341 ttattgagat tgaagccgct tttttgaaac tcttcctcaa cttccctccc tacttcgtaa
    14401 ttatcgatgc cgattgccgg accaattgct gcgagaatgt cccgggtttc agttccaaag
    14461 tttttcttca tttcaagaag tgtcttttca acgattctcc ctgaggttcc tttccatccg
    14521 gcgtgaatag caccgatagc ttcgtttttc cggtcgaaca acaacagcgg aacacagtcg
    14581 gccgtggtga cacacaagaa gtaacctttc tcctgtgtaa cggatgcgtc gtatccgtag
    14641 agcctttcaa ttttttctgc attggagaga ttcagaaact cagcgtcaat aagcagtacc
    14701 ctattaccat gggtttgatg cggagttatc agatcattgg cctctttgta gaactttcgg
    14761 gccacaatgg atctgttctc aaaaatattc agcggattat catcggagta atttcccagg
    14821 tttaacgacc ggaattcacc gcggctcacg ccgcctgccc gtgtagtaga gaaatgaagc
    14881 aaccgctgtt cattggagaa aatagtgaag aaaagcaggt ttgaataatt ggggtggcga
    14941 aacataattt agaaatcctc ttcgtaataa ttttcttctt cctccccgta ttcatcgtcc
    15001 aaatcctctt caatttcttc ttcgggttca aactccaggg attgaacgtc caggcgtctg
    15061 tgaacaagcg actcccccga tgtggaaacg tggatggtct cttctgaatt taattccctc
    15121 cagagaatat cctttaacgc agggattccg tatccggtga tggacgatat gaaaacatag
    15181 ggaattttcg gtaagtcata ggaaatctcg tgtattaatt cgtcgtccag catatctgct
    15241 ttagagattg cgagaacacg atgtttgtct agcaactcgg gattatattt ggtgagttcg
    15301 ttcaacagaa tgttgtattc ttcgtgaata ttttcggcat cggcaggtac cacaaacaaa
    15361 agtagcgagt tcctctcaat atggcgtaaa aatcgcagac cgagtccttt cccttcgctg
    15421 gctccttcta tgatgcctgg aatatccgcc atcacgaacg attttccttc gcgataactt
    15481 actatcccca aattgggttc caatgtggtg aatggataat taccaatctt tggcttcgct
    15541 gcagacacca cggacaggag tgttgatttt ccggcgttag gaaagccaac caatcccacg
    15601 tcggcgagca atttcaattc aagaataatc catttctctt cgtaaggctc tcccggttgg
    15661 gaataacgcg gtgcctggtt ggttgccgat ttgaagttcg tgtttcccaa tcctccacgt
    15721 ccacccttca gcaaaactat ttcttgtcca tcgtcggtaa tgtcgcacag gtattcaccc
    15781 gtatgagcgt cataggccac agttccgcac ggtacttcaa tgattttact gtcgcctctc
    15841 ttgccagagc tgttgcgtct tgagccgctt tcaccatgtc cggcaaagat atgccgttgg
    15901 aatttcaaat ggagcagtgt ccagtagttt ctgttgccgc gtaagattat atgtcctcca
    15961 tcgccaccgt ttccaccgtc aggcccgccc tttgggatat atttttctct tctgaaatgg
    16021 gttgaccctc gtcctccctt tcccgatctg caaaagattt ttacataatc aacaaaattt
    16081 gtttcagcca ttttatctgc cagtaagatt atcaaccact tctactatcg aattaaagat
    16141 ctcctcgata gctccctcac cgggtatgca tactaatttg ttctgtccgg cataatactc
    16201 tttcaacggt tctgtttgct ggtggtatac gttgagcctc gatttgatgg tatgcccgtt
    16261 gtcatcggag cgacccgata gttgcccgcg tttcagcagc ctgtcaatca gttcttcgtc
    16321 cttaacctcc aggcttagta cagaggtgat gtccaaaccg tttttcttca ttttgttgtc
    16381 caaggcttct ccttgcggaa gtgtacgggg aaatccatcg aaaataaatc cttttttatc
    16441 caggtttttc cggatcagat cttccagcat ttcgatcata atctcgtcgg gaaccaaatg
    16501 tcctttcgaa atatattgat ctgctatttt gcctaacgct gagccagcct ttatttcaaa
    16561 tctcaatata tctcccgttg atacgtgctt cagtccgtat tttttaatca ataaatcact
    16621 ttgtgttcct tttccagaac cgggagcgcc aaaaatcacg atgtttaaca taaaaattat
    16681 ctatgagata agtttgtgta ataatccata taaagatata actaacagat atccaacgat
    16741 gatttgtttc gtattattta attgatattt gttgtagcgt tgaaaatttc atcgttacaa
    16801 tcaaattaca attgaatatc tattgaatta ctttaatgat tacaaagata aggttttttt
    16861 tgcttttaat ttatcgtttt gaacttaatt agggcgatat ctgttttaat gtcagaaaac
    16921 tttttaaaaa aatatcgtat gaacaaacaa gagtttaagc aaaagacgca ggagatattg
    16981 gatcagctcg atgcaaaaat tgatgaaatg aagcaaggca tcagcaacat taccgaagac
    17041 gccaaagaag aatatgccga gcaaatagaa aaattgaaaa gcttgaaaga tgaactgtcg
    17101 gaaaagttgg gaaagtttga tgatattgct gaaaataaat gggatgtagt tagagaaagt
    17161 gcctccaact tcttttctaa agtaggggaa gcgtggaaag aagattttga gcgggttaaa
    17221 caagctttca aaaaagagac tgacacagct tcaggtatgg cacaggatac ggaaacgggt
    17281 gctaaagagg actaggcata aaaaaactcc cgaatttcgg gagttttttt atgcctctgc
    17341 gagagataat tatttgatac ccagtttggt tttaaccaaa ggagtggcat cctctgcgtt
    17401 tgtagcattg aagtaaaccg ggtttccagc tgccaggtca aaaatatagg tgtatccttt
    17461 ttcatcaccc actgctttaa tggcggtagc aatcttttgt tgaaccggag ccagcagctt
    17521 ctggcgtaac tcttccattt cccgttggct gttttgcaaa aacatttgat acctttcatt
    17581 aatctgatcc aactgtttct gttgatcggc tttaacggtc tcggatgaag tgggtgcc
//
LOCUS       DAOSDR010000008        17244 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrimonas sp. isolate AMR_MDS_1826
            NODE_1348_length_17244_cov_3.515039, whole genome shotgun sequence.
ACCESSION   DAOSDR010000008 DAOSDR010000000
VERSION     DAOSDR010000008.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863355
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrimonas sp. (wastewater metagenome)
  ORGANISM  Petrimonas sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Dysgonomonadaceae; Petrimonas.
REFERENCE   1  (bases 1 to 17244)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17244)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:10:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,720
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 35
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17244
                     /organism="Petrimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1348_length_17244_cov_3.515039"
                     /isolate="AMR_MDS_1826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2023866"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..961)
                     /locus_tag="PLI30_00720"
     CDS             complement(<1..961)
                     /locus_tag="PLI30_00720"
                     /inference="COORDINATES: protein motif:HMM:NF028442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HOI78108.1"
                     /translation="MKINNKAAITNPSKQMLFDSKFIIILITFFLINGCKTERDIYKT
                     TFKTSYSELLDDFKSPPNDAKIRAYWWWLNSNVTKDCITKDLEAMKNNGYGGAMIFDA
                     GSSAYDVAHKTEAGPAFLSKEWLELFGHAVSEADRLELELSINVQSGWNPGGPSVTPE
                     LALKKITWSEMTINGPEAVNRELPLPLGENILEDVKIIAVKQSGDTVPQSDRIKNIKL
                     KTLEQRLGSAGIYPLHLLREDFEGKGNPINKSDIIDITEYFDGENLKWNAPDGTWSVL
                     RFVMINTGKGVSTASDGWEGLAMDHLNKEALLKYNQDVISKLIQ"
     gene            complement(1390..1998)
                     /locus_tag="PLI30_00725"
     CDS             complement(1390..1998)
                     /locus_tag="PLI30_00725"
                     /inference="COORDINATES: protein motif:HMM:NF036640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NigD-like C-terminal domain-containing protein"
                     /protein_id="HOI78109.1"
                     /translation="MKRNYVVYLTVFFLLASACEKEPERIDNFFVEFATVIKPVERIT
                     FQLDNFKTLIPIELKDYSGKNGQRVILNYTPLRGDTVKINSVNDIFTGVVQESNAVTN
                     LIKDPVKVQSVWVDGNYLNLILEIEYFDKTHVIGLFRDTQSSTIDLHFSHSKENDPPG
                     YTQKLYASFSLSAIKSPNGSPTPFRFHIQTHTGVRVFELKVK"
     gene            complement(2027..3172)
                     /locus_tag="PLI30_00730"
     CDS             complement(2027..3172)
                     /locus_tag="PLI30_00730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019539047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HOI78110.1"
                     /translation="MQNFDDIRPLYDGEVPSVIRSLINDPYFRRAAEPLIKPITWEIF
                     SGAMLACKSINDFQRTIIYPFMKQLIAKTTSELTGIGFEEFKDGSSHIYISNHRDIVL
                     DAAFLNILFFDKGIDTSEIAIGDNLLIYNWITDLVRLNKSFIVKRRVSVREMLDTSRH
                     LSEYIFDTITRRRQSAWIAQREGRAKDSDDKTQASLLKMLTLYNSSKPAEALRSLDII
                     PLSITYEFDPCDYLKAKEYQLKRDNPGYKKSQADDIENMRTGILGYKGKVFFKFGNRI
                     NNTLSRIDEKTSRAQVLETATQAIDREIYKNYVFFPMNYIAYDLMENSNLFAARYTDE
                     DKAAFDNYIDGQIAKIDIPGKDYRFLREKLIGMYGNTVKNFVSAGKI"
     gene            complement(3176..4159)
                     /locus_tag="PLI30_00735"
     CDS             complement(3176..4159)
                     /locus_tag="PLI30_00735"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019539048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HOI78111.1"
                     /translation="MKKNIAIIWGGYSSEKEVSERSARGIYSFIDKSRYNLYKVKIDK
                     EVWEAEYGNETFPIDKNDFSFTANGTRTAFDFAYITIHGTPGEDGVLQGYLDMLNIPY
                     SNCGVLASALTFSKFTCNHFLKSFGFNVAESVILRSRESYDTGSIVHQLGLPLFVKPN
                     IGGSSFATTKVKELSLLQQAIEEAFNEASEVMVESFIAGTEVTCGCYRAGNGFQHLPL
                     TEVVTQNEFFDYNAKYKGEVEEITPARIPDDMAAQVQEQTERIYRLIGAKGIIRADYI
                     ISGGIPVLLEVNTTPGMTETSFIPQQVRAAGLNISDVMTDIIEYEYNRLKK"
     gene            complement(4199..5266)
                     /locus_tag="PLI30_00740"
     CDS             complement(4199..5266)
                     /locus_tag="PLI30_00740"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006842226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HOI78112.1"
                     /translation="MQDFPEDEDELTGISAQEEKFEHFRFVADKGQSLLRIDKFLSDR
                     IEGISRNRIQQAADALCILVNGITVKSSYRVKPLDVVTVVMDRPRRELEILPEDIPLN
                     IVYEDNDLMVINKPPGMVVHPGHGNYTGTLVNAIAFHLKYEHGMDMTDPRLGLVHRID
                     KDTSGLLLIAKTPDAKTYLSAQFFNKTTKRRYTALVWGNVTSDEGTITGNIGRDPKDR
                     MQMRVFTDGEQGKPAVTHYKVLERFGYVTLVQCELETGRTHQIRVHMKHIGHTLFNDE
                     RYGGNEVLRGTHFAKYKQFVYNCFTVCPRQCLHAQTLGFIHPRTRKEMFFESELPQDM
                     QEVIGRWRTYTAARETGEHQI"
     gene            complement(5331..6005)
                     /locus_tag="PLI30_00745"
     CDS             complement(5331..6005)
                     /locus_tag="PLI30_00745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019539050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PASTA domain-containing protein"
                     /protein_id="HOI78113.1"
                     /translation="MSTKPTSKKFPGNNILLNVVAIGVVAILLFVLTLLFLNVYTRHG
                     QNVVVPQLQGFQEEEAEAILKSKGLDIQVVDSIYKKDAVPGSIIDQTPKPNNKVKEGR
                     DIYVTIYARNPQQVSVPGLVDFSARQAIALLNSIGFTQIAIEEIPAQYSGLVISVEYR
                     GKKLIPDEQIPAGSPLKLVVGSGMSEDSLSINREIVVSPGVTGTIPDSSRNVPVKPSN
                     MDESFF"
     gene            complement(6166..6306)
                     /gene="rpmH"
                     /locus_tag="PLI30_00750"
     CDS             complement(6166..6306)
                     /gene="rpmH"
                     /locus_tag="PLI30_00750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005840676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HOI78114.1"
                     /translation="MKRTFQPSNRKRRNKHGFLSRMATKNGRKVLASRRAKGRVRLTV
                     SD"
     gene            6550..6621
                     /locus_tag="PLI30_00755"
     tRNA            6550..6621
                     /locus_tag="PLI30_00755"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6583..6585,aa:Glu,seq:ttc)
     gene            6654..6908
                     /gene="rpsT"
                     /locus_tag="PLI30_00760"
     CDS             6654..6908
                     /gene="rpsT"
                     /locus_tag="PLI30_00760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019539053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HOI78115.1"
                     /translation="MANHKSAEKRIRQTKVRRLSNRYVSRTTRNSVRALRALTSKEEA
                     AAMYPGVCSMLDKLAKKKVIHQNKANNLKAKLAAHISKLN"
     gene            7268..7573
                     /locus_tag="PLI30_00765"
     CDS             7268..7573
                     /locus_tag="PLI30_00765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019539054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dabb family protein"
                     /protein_id="HOI78116.1"
                     /translation="MIKHIVLFKLAEEAEGNSKAANAVIIKQRLEALKDLIPEILKME
                     VSINHADAPAGNYDIMLDSEFRNLDDLHTYISHPEHQKVGAFIGKVRIGRAAIDCEF"
     gene            7720..9678
                     /gene="gyrB"
                     /locus_tag="PLI30_00770"
     CDS             7720..9678
                     /gene="gyrB"
                     /locus_tag="PLI30_00770"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018964978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009330 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) complex [Evidence
                     IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HOI78117.1"
                     /translation="MTEEEKKSASGNYSAQNIQVLEGLEAVRKRPAMYIGDVSEKGFH
                     HLVYEVVDNSIDEALAGYCNEINVTINEDNSITVTDNGRGIPVDYHEKEKKSALEVVL
                     TVLHAGGKFDKGTYKVSGGLHGVGVSCVNALSMHLKAEIHRDGKIHVQEYSRGKPYSD
                     VQVIGQTDKTGTIITFKPDDTIFIVSEYRYDILATRLRELAFLNAGVTLTLTDKRTPR
                     EDGTFKTERFYSENGLEEFVRYIDKAKEPLISEPIHIVTEKQGIPIEIAMTYNSTYNE
                     NIFSYVNNINTIEGGTHLTGFRRGLSRTLKKYAEDSKMLEKVKVEISGDDFREGLTAV
                     LSVKVAEPQFEGQTKTKLGNSEVIGAVDQATGEALNNYLEEHPKEAKIIVEKVILAAT
                     ARHAARKARELIQRKSPLSGGGLPGKLADCSDKDAEKCEIFIVEGDSAGGTAKQGRDR
                     MFQAILPLRGKILNVEKVMTHRVYENEEIRNIYTALGVTIGTEEDSKALNIEKLRYHK
                     VVIMTDADVDGSHIATLILTFFFRYMKPLIENGYVYIATPPLYLCKKGKIEEYCWDDR
                     QRQAFIDKYADGNENAVHSQRYKGLGEMNAEQLWETTMNPEKRTLRQVTIENAADADI
                     IFSMLMGEDVAPRREFIEENATYAHIDA"
     gene            9691..10599
                     /locus_tag="PLI30_00775"
     CDS             9691..10599
                     /locus_tag="PLI30_00775"
                     /inference="COORDINATES: protein motif:HMM:NF036772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HOI78118.1"
                     /translation="MLQPPILQTGDKAVILSPAGKIDAGLVAKAADLLCDWGLGVEIS
                     ENALCRTGRFSGFVEQRLADLQRAMDDPEVKLIFCSRGGYGTVHLLDKLDFTGIRQYP
                     KWVVGYSDITVLHATLQQHGIMSVHGPMAKHFAEEGPGDISVRFTKAILAGEPVFYDI
                     PVAKYAYLNRKGKASGRLFGGNLSVFCGILGTRFAYLPKNGILFIEDIGEEPYKIDRY
                     INQLKLAGIFDRISGLIVGQFSDYTEDNDMYSALYESIASVVTGYDFPVCFDFPAGHV
                     KHNFPLVMGKTAKLVVKEDQLIFKQS"
     gene            10609..12123
                     /locus_tag="PLI30_00780"
     CDS             10609..12123
                     /locus_tag="PLI30_00780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005863241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium/proton antiporter"
                     /protein_id="HOI78119.1"
                     /translation="MEVSTELILLIGSFLFFVSMLVGKAGHKFGVPVLLLFLLVGMIF
                     GGDGFGLNFENIQIAQAIGTVCLTIILFSGGLDTKFREIKPVIQPGVVLATLGVFITA
                     IITGIFIWWLSDQVYTGLGVGFLTAMLLASTVSSTDSASVFGILRSKGLMLKNNLRPL
                     LELESGSNDPMAYMLTVTFISLINSGNDPNYVIVAVNIVVQLVVGALLGYFLGRFSVV
                     IINRIRMDNTSLYPVLLLITGIFIFAVTYYLKGNGYLAVYIAGLVIGNSKFAHKRTSM
                     SFMDGFAWMSQILLFLTLGLLVNPSELVPVLIPGIIIALFMIFIARPITVYLCLIPFK
                     RISFRDKAYISWVGLRGAVPIIFAILPLAAGVPGARTVFNIVFVITIVSLLYQGTSLP
                     IVAKWLGLAEKPSKYKSFEDFDVEFSEEIKSAMTEISITEETLKHGKNLMEMPLPDNT
                     LAVMVKRGERFFVPTGQTALMDGDKLLVISDDEEALKETYKNIGISNFTYQKND"
     gene            12123..13148
                     /locus_tag="PLI30_00785"
     CDS             12123..13148
                     /locus_tag="PLI30_00785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019539058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HOI78120.1"
                     /translation="MEKLNSLFIVLLVGVGLVFSVSCNSCQSQKNAGKETTAIAYAKV
                     SPDFNSDSAYRFVEKQVSFGPRVPGTTAHKACGDYLSEKLAAFGADIFEQKAHVTHYN
                     GQNIQIRNIIGSFQPEKEKRILLFAHWDSRPFADEEPDPDTQNRPIPGADDGASGVGV
                     LLEIARQLQLQPPGIGVDIIFFDLEDWGQPAFDKNYLSGEWWCVGSRYWSENPHKNNY
                     KASYGILLDMVGAANATFLREGYSMQYASNVVSKIWSIASHMGYGKFFVQRNGSYITD
                     DHVPVIEKRGIPCANIINLKDTDNGFAPHWHTHNDDMRNISKATLQATGQTVLEVVYR
                     ELDENNI"
     gene            13145..13579
                     /locus_tag="PLI30_00790"
     CDS             13145..13579
                     /locus_tag="PLI30_00790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002558161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SufE family protein"
                     /protein_id="HOI78121.1"
                     /translation="MTINETEQEIIDEFSIYDDWMDKYAYIIEQGNQLAPLDEKYKVP
                     ENIIQGCQSRVWLQTDYRDGRLYLQAESDAVIVKGLLALVLRVFNHRTPDESLASDLR
                     FMKEIGLTEHLSPTRSNGLLSVIKQIRFYAMAYKAKSDSEKR"
     gene            13647..>14369
                     /locus_tag="PLI30_00795"
     CDS             13647..>14369
                     /locus_tag="PLI30_00795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019539602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M3"
                     /protein_id="HOI78122.1"
                     /translation="MTTASMADENPFFKPYDTPYGTPPFDKIKIEHYEPAFDEAIRQH
                     KVEIETIAANPFAPTFQNTIAAMEYSGEMLNRVSGVFFNLLSAESNDEMMMISQRLSP
                     KLSEHSNNINLNEKLFARVKTVYDNRLTSGLLPEQIRLVEKYYEQFENSGATLSAGDK
                     ETYRKLSMELSKTTLDFGQNNLKETNRFEMLLTDEADLAGLPVSILEAAVAKAKSKGK
                     EGWMFDLSAPSYIGFMKYSTRRD"
     assembly_gap    14370..14469
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <14470..15859
                     /locus_tag="PLI30_00800"
     CDS             <14470..15859
                     /locus_tag="PLI30_00800"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009018586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="M3 family metallopeptidase"
                     /protein_id="HOI78123.1"
                     /translation="DLSAPSYIGFMKYSTRRDLREKLYMAYNTKSVMGGEFDNKENIK
                     RIVNLRLQIANLLGYRNYAAYALKNRMAKNEEGVYSLLEELARAYGETARREVKEVEA
                     FAVKMEGKPIEIQPWDWSYYSDKLKDDRFDLNDEMTRPYFELENVKKGVFGLATDLYG
                     ITFVKNPSTPVYHPEVEAFDVMDANGDFLAVLFTDFHPREGKRSGAWMSSFKSQFVKN
                     GVDSRPHITIVMNFTRPTETKPALLTFDEVETFLHEFGHALHGMLAKSTYETLSGTSV
                     YRDFVELPSQIMENWLVEKEYLDKFAFHYQTGEKMPAQLVQKIIDAANYNTGYLTLRQ
                     LSFGYLDMAWHTLQQPFDGDIREFEQKAMRPVQLLPVVPETSMSTAFGHIFSGGYAAG
                     YYSYKWAEVLDADAFAAFKENGIFDKKTAQSFRVNILERGNTEEPMTLYKRFRGHEPT
                     VGALLERNGVKQ"
     gene            16100..16414
                     /locus_tag="PLI30_00805"
     CDS             16100..16414
                     /locus_tag="PLI30_00805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019539603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HOI78124.1"
                     /translation="MNIFVAGLSYQINDADLKELFEEYGEITSAKIITDRETRRSKGY
                     GFVEMADEDGQRAIEELNGAEYDGRTLSVSEARPRAEGDNQRGNRGGGNRGGYGNRER
                     RY"
     gene            16528..17214
                     /gene="tsaB"
                     /locus_tag="PLI30_00810"
     CDS             16528..17214
                     /gene="tsaB"
                     /locus_tag="PLI30_00810"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008862170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HOI78125.1"
                     /translation="MPTIIHIETATEVCSCALSSGGKVLLNRENLNGQSHATSLGVFV
                     EEMIQFVRDNGMKIDAVSVSSGPGSYTGLRIGVSEAKGLSYGLEVPLIAIPTHKIMAW
                     MMRTGIEPDHLLCPMIDARRMEVYATIFNANLEVIRATSADIVDKESYAELLKNQKIL
                     FFGNGAEKCKGIILHPNASFIPAVKPKAEAMTALAENAFAAADFVDSAYFEPFYLKEF
                     VATVPKNKII"
BASE COUNT         4669 a         3588 c         3967 g         4920 t
ORIGIN      
        1 tctgaatcag cttggatata acatcttgat tgtatttcaa caaagcctcc ttattcaggt
       61 ggtccattgc caatccttcc catccgtcac tggcagtaga tactcctttg ccggtattta
      121 tcattacaaa acgaagaaca gaccatgttc cgtcaggtgc attccattta aggttttctc
      181 catcaaagta ttccgtaata tctattatat cactcttatt tatcggattt ccttttccct
      241 caaagtcctc tctcagcaga tgtaggggat aaatgccggc agagccgagt ctctgttcca
      301 aagttttgag ttttatgttt ttgattctgt cggattgcgg aacggtatca cctgattgct
      361 ttacggcaat aattttcaca tcctcaagga tattttctcc gagagggaga ggcaattccc
      421 tgttaacagc ttccgggcca tttatcgtca tttcgctcca ggttattttt tttaaagcaa
      481 gttcaggtgt tacggaaggc ccgcccggat tccatccgct ttggacattt atcgatagtt
      541 ccaattcaag ccggtctgcc tcactgacgg cgtgcccaaa aagttcgagc cattctttgc
      601 ttagaaacgc cggccccgct tctgttttgt gtgccacatc ataagcagaa gacccggcat
      661 cgaagatcat tgctccgccg tagccattat tcttcatggc ttccaggtct ttggttatac
      721 aatctttcgt cacattgctg ttgagccacc accaataagc ccttattttt gcatcgttag
      781 gtggagactt aaaatcatcc aataactcag agtatgaagt tttaaacgtt gtcttgtaaa
      841 tatctctttc ggttttgcaa ccattgatca agaaaaaagt aatgaggata attatgaatt
      901 ttgaatcgaa cagcatctgt ttacttgggt ttgtgattgc tgctttgtta tttattttca
      961 tgttgcaaga gttttggatg agtaactttt cttttacagg atattagtat aataactatc
     1021 cgaatttcat aaatgccgga tttgaaagtc acaaaaataa aatatcctcc aactggaaaa
     1081 ctgactaaaa tgacacataa gctgtctttt ttgatatttt atctttcgaa cttttatgtt
     1141 aatgatgtag cgagatgcga tattgccctt ctttataacc cgaaaggcaa ttaaaaaaag
     1201 ttagaaatac aaaactacaa ttggatgttt tttgcaatag gataaatttg ccgggaaatc
     1261 tgtaggggga tacaataatt ccttattaaa aatatgaaaa ctgtgaaaaa tgatatagta
     1321 attgaatgtt tttgctggcc ggatccaagg tttattgctg taaatatgtg ttaaaaatag
     1381 aggggtggat cattttactt ttagttcgaa tacgcgtacg ccggtgtgcg tttggatatg
     1441 aaaccggaaa ggggtggggg aaccgttggg ggatttaatg gcggaaagtg agaaagaagc
     1501 ataaagtttc tgggtgtatc caggtggatc gttttctttt gaatggctga agtgcagatc
     1561 gatagtggaa gattgcgtat cgcgaaacaa tccgataacg tgagttttgt cgaagtattc
     1621 tatttcgagg atcagattca ggtagtttcc gtccacccaa acgctttgca cttttacagg
     1681 gtctttgatg aggttggtca cggcgttgct ttcctggact acccccgtga aaatatcgtt
     1741 aacgctgttg attttcaccg tgtcgccgcg taaaggcgtg tagttgagaa tgacacgttg
     1801 cccgtttttg cccgaataat ctttcaattc aatcgggata agggtcttga agttatccag
     1861 ttgaaacgtt atccgctcaa ctggttttat tactgtggca aactctacga agaagttatc
     1921 gatacgctcc ggttctttct cacatgcact tgcaaggaga aaaaaaaccg tcaggtatac
     1981 aacataattc ctcttcattg ttttagctta ctgccattat ttttacttat atttttcccg
     2041 ccgaaacaaa attcttcact gtattcccgt acatcccgat tagcttttca cgcagaaaac
     2101 ggtagtcttt gccggggata tcaatttttg caatctgccc gtcgatatag ttatcgaaag
     2161 ctgccttgtc ttcatcggta tatctggcgg caaacaagtt gctattttcc atcagatcgt
     2221 aggcaatgta gttcatcgga aaaaagacgt agtttttgta aatctccctg tcgatggcct
     2281 gggttgcagt ttccagtacc tgtgcccggc tggtcttttc atcgatacga gataatgtgt
     2341 tgttgatccg gtttccgaac ttgaaaaaaa ctttcccttt ataacccaaa atgcccgttc
     2401 gcatattttc gatatcgtcg gcttggcttt ttttatatcc ggggttgtct cttttaagct
     2461 ggtattcttt ggctttgagg taatcgcacg gatcaaattc gtaggtgatg gaaagaggta
     2521 ttatgtcaag cgacctgagt gcctcagccg gctttgagct gttgtacagt gtcagcatct
     2581 tcagcaggct tgcctgggtc ttatcgtctg aatcttttgc gcggccttcg cgttgggcaa
     2641 tccaagcaga ttgacggcga cgggtgatgg tgtcgaaaat atactccgac aggtgtctgg
     2701 aggtatccag catctcacgg actgaaacgc ggcgtttcac gataaaactt ttgttaagcc
     2761 ttaccaaatc agtaatccag ttatatatca gcaaattatc tccgatggca atttccgacg
     2821 tatctattcc tttatcgaaa aagagaatat tgaggaaagc tgcatccagt acaatatcac
     2881 ggtggttgga aatgtaaatg tggcttgaac cgtctttgaa ttcttcaaat cctataccgg
     2941 taagttctga tgtagttttg gcaataagct gtttcataaa aggataaatg atggttcgct
     3001 gaaaatcgtt aatcgatttg caagcgagca tggcaccact aaatatttcc cacgttattg
     3061 gcttaatcag gggttcggcc gctctgcgaa agtagggatc gttgataagg gaccgaataa
     3121 ctgaagggac ctcgccgtcg tacaaagggc ggatatcatc gaaattttgc atagtttatt
     3181 tttttaaccg gttatattcg tattcaataa tatcggtcat cacgtcagaa atgttgagcc
     3241 cggcggcgcg tacctgttga ggtataaaac tggtctcggt cattccgggt gtagtattta
     3301 cttcgagtag aacgggaata ccgccggaaa tgatgtaatc tgccctgatg ataccttttg
     3361 caccgataag tcgatagatc cgttcggtct gttcctgaac ttgtgctgcc atgtcgtctg
     3421 gaatccgcgc cggagtaatt tcttcaactt cccctttata tttggcgtta taatcgaaaa
     3481 actcattctg ggtaaccact tcagtaagcg gcagatgctg aaatccattt cctgcccgat
     3541 agcatccgca agtcacctct gtccctgcaa taaaactttc gaccatcact tcggaagctt
     3601 cattaaaagc ctcttctata gcttgttgca ggagcgatag ctcttttacc ttggtcgtag
     3661 caaagctgga tccgccgata ttgggcttta caaatagagg gaggcctaat tgatgaacga
     3721 tcgatccggt atcgtacgat tccctggaac gaagtatcac cgattcggcc acgttgaacc
     3781 cgaagctctt caggaagtgg ttgcatgtga atttactgaa agtcaatgca gatgctagca
     3841 ctccacaatt ggagtaggga atgttgagca tatcgagata gccctgtaaa acaccatctt
     3901 cccccggggt gccgtgaatg gtgatgtagg caaaatcaaa cgctgttctt gttccattcg
     3961 ccgtgaagct gaaatcgttc ttgtcgatgg gaaaagtttc gtttccgtat tccgcttccc
     4021 agacttcttt atcaattttt accttataaa ggttatacct gcttttatca ataaaagagt
     4081 aaatgccccg ggcgcttctc tctgaaactt ccttttcaga cgaatagcct ccccaaatga
     4141 tggcaatgtt ctttttcata tttttttgtt gtggtttgag gtgtgagtta ttatgcattt
     4201 atatctggtg ttctcccgtt tcccttgcag ccgtatatgt gcgccaacga ccaataacct
     4261 cctgcatatc ctgcggcagt tcgctctcaa aaaacatctc ctttcttgta cgaggatgta
     4321 tgaatccgag cgtttgtgcg tgcaggcatt gtcgcggaca aacggtgaaa cagttgtaca
     4381 cgaactgttt gtattttgca aaatgtgttc cgcgcagtac ttcgtttccc ccgtaacgtt
     4441 cgtcgttgaa cagcgtgtgt ccgatgtgtt tcatgtgtac acgtatctga tgtgtgcggc
     4501 ctgtttccag ttcgcactga acaagcgtta cgtaaccaaa tctttccagt accttgtagt
     4561 gagtaaccgc aggctttcct tgttcgccat cggtgaaaac gcgcatctgc atccggtctt
     4621 ttgggtcacg accgatattc ccggtaatgg tgccttcatc gctggtaacg tttccccaca
     4681 ccagtgccgt atatctcctt ttagtggttt tgttgaaaaa ttgtgcggaa aggtatgttt
     4741 tggcatcggg tgttttagca ataagtaaca agcccgatgt atccttgtcg atgcggtgta
     4801 ccagtcccag gcgcgggtcg gtcatatcca tgccgtgttc gtatttcaaa tgaaacgcta
     4861 tcgcgttaac caatgtacct gtatagttac cgtgcccagg gtgtactacc ataccaggag
     4921 gtttgtttat aaccataagg tcgttatctt cgtaaacgat gttgagcgga atatcttccg
     4981 gaagtatttc cagctccctt cgcgggcgat ccataacaac cgtgactaca tccagcggtt
     5041 taaccctgta gcttgatttt acggtaatcc cattcaccaa aatacataga gcatcggcag
     5101 cctgctgaat ccggtttcgt gaaattcctt cgatacggtc cgataaaaat ttgtcgattc
     5161 gcagtaaact ttgtccttta tcggctacaa atcgaaaatg ttcaaacttt tcttcctgcg
     5221 cggaaatgcc ggtcagctca tcttcatctt ccggaaaatc ctgaatatcg tatgggtcct
     5281 cttctttcac gtgtacatgt tttagctctt cgcaacttgt cgcgcctctt ttaaaagaac
     5341 gattcatcca tgttggacgg ttttacgggt acatttcgtg aagaatcggg aattgttccg
     5401 gtaactccgg gagaaaccac aatttccctg tttatgctta gcgaatcttc tgacatcccg
     5461 cttcctacga ccaatttcaa aggtgatccg gcaggaattt gctcgtcggg tatcagtttt
     5521 ttcccccgat attcaaccga aataaccaat ccgctatact gggcggggat ttcttctata
     5581 gcaatttgcg tgaatccgat ggagttgagc agtgcaatag cctgtcgcgc ggaaaaatca
     5641 accagccccg gaacggaaac ctgttgcggg ttgcgggcgt aaatggtgac atatatatcc
     5701 cggccttcct ttactttgtt gttcggcttg ggtgtttgat caataatcga accgggaaca
     5761 gcgtcctttt tgtaaatcga gtcaacgacc tgaatgtcca accctttgga tttcaaaata
     5821 gcttcggctt cctcttcctg aaatccttgt agctgaggaa ccaccacgtt ttgtccgtga
     5881 cgggtgtata cattcagaaa aagtagggtg aggacgaaca acaaaatagc tacaacgcca
     5941 atggctacta cgttgaggag aatgttgttg cccggaaatt ttttactagt cggttttgtg
     6001 ctcatatact tgaatatgat gttgaaattt cattgcaaaa gtacaaaaaa aaatggttgc
     6061 ctttgtcttt tccggacaaa gagtttggag tatatgcgga acacaacaaa aaaagcagga
     6121 atatgcatca catattcctg cttcccataa aaggcctttt tcggattaat cggatacggt
     6181 caatcttact cttcctttag cacggcggga ggccaatact tttcttccgt tcttggtcgc
     6241 cattctcgaa aggaacccgt gtttgtttct tctctttctg tttgagggtt gaaatgttct
     6301 tttcatttta tctgatgttt tatttgttgc ttaaatattg gggtgcaaaa ttagaaatta
     6361 tttttcaaat atccaattta ttgggcttaa aattctgtat agctttgttt ttttataatc
     6421 aaggtataaa tttgttgctt tttttgagaa aaaaatcggg caaaattttg tcggtctcaa
     6481 aaaaggttct atctttgcac tcgcaatttg aggaaaggtt aatttttctt tcgggttcgc
     6541 aactcaaatg gcccattcgt ctatcggtta ggacgcaaga ttttcattct tgaaagaggg
     6601 gttcgattcc cctatgggct acaataaaaa agatttaaac aagtcaaaca ataatggcaa
     6661 atcacaaatc agcagaaaaa aggatcagac aaacaaaagt tcgtcgttta tcaaatcgtt
     6721 acgtttcacg cacaaccaga aattcggtac gcgcgttgcg cgctttgacc tcaaaggaag
     6781 aggctgcagc catgtatccg ggggtttgct caatgttgga taaactggca aaaaagaaag
     6841 ttattcatca aaacaaagcc aataatttaa aggctaaatt agctgctcac atcagcaagt
     6901 tgaactgatt tttttatctg ttaagaataa acggagccgg tcacaagtgt gtccggtttt
     6961 tttttagcct atacctgata atggcagttg cttttcattg cgtttacttt ctgcctttgt
     7021 attttcgcct tcttttctta ctggcattgt agtactgata aaacgccaat gcgatcactg
     7081 cgatgcctac gatggtgata attgttccta tattcatagc tgtttaattg atttgtgatt
     7141 gaagcaaagt tacgaaaaaa cgagaaactt cttcgatttt ctcaggcaat atttttttag
     7201 taattttgag ttttgtctaa gctccagatt ttgctaggat gacaggataa attaaagcac
     7261 ccaatttatg attaagcaca ttgtactctt taagttggct gaagaagccg aaggtaactc
     7321 aaaagccgca aatgcggtaa taatcaagca gcgcctcgag gcactgaaag atttaattcc
     7381 cgaaatttta aaaatggagg tatcgataaa tcatgccgat gctcctgcgg gtaactacga
     7441 tattatgctc gacagcgagt tcaggaacct ggatgattta catacctata tttcgcatcc
     7501 ggaacaccag aaagtagggg cgtttattgg taaggtgcgg attggccgtg cagctatcga
     7561 ttgcgaattt taaacggctt ttcaatagtt ttccctctgc taaaaaaacg aaaaacggtt
     7621 ggcaggacgg cgaatttttc gtatatttgt tcggtttttt aaccaaaaaa cggataggga
     7681 taaattataa attctcagag agatatttaa aggataaaaa tgacagaaga agaaaaaaaa
     7741 tcggcaagcg gtaattattc cgcgcaaaac attcaggtac ttgaagggtt ggaagcggtt
     7801 cgtaaacgcc cggctatgta tatcggagac gtaagcgaaa aaggatttca tcaccttgtt
     7861 tacgaggtgg ttgataattc cattgacgaa gcgcttgccg gatattgcaa tgaaataaat
     7921 gtaaccatca atgaggataa ttccattact gttaccgata acgggcgcgg aattccggtg
     7981 gattaccacg aaaaggaaaa aaaatcggcg ttggaagtcg tgcttaccgt gcttcacgcc
     8041 ggggggaagt ttgacaaggg aacgtacaag gtttctggag gcttgcacgg tgtgggcgtg
     8101 tcgtgtgtga atgccctttc gatgcatctc aaagccgaaa ttcatcggga cggaaaaatt
     8161 catgtgcagg aatattcgcg cggaaagcct tattccgatg tgcaagtgat cgggcagacc
     8221 gataaaaccg ggaccataat cacctttaaa cccgatgata ctatctttat cgtttcagaa
     8281 taccgctatg acattttggc gacacgactt cgtgaactgg cttttttgaa tgccggggta
     8341 acgcttaccc ttaccgacaa gagaacgccc cgcgaagacg gtactttcaa aacagaacgt
     8401 ttttattctg aaaatgggtt ggaagagttt gttcgttata tcgacaaggc caaggagccg
     8461 ctcatttcgg aacccattca cattgtaacc gaaaaacagg gcattcccat cgaaatagcg
     8521 atgacgtaca attccacgta caacgaaaac atattctctt acgtaaataa catcaacacc
     8581 atcgaaggag gtacacacct taccggcttc agaagaggac tttcgcgcac actgaagaaa
     8641 tatgccgagg attccaagat gctggaaaag gtaaaggtgg aaatcagcgg tgatgacttt
     8701 cgtgaagggc ttacagccgt gttgtcggta aaagtagccg aaccgcaatt cgaggggcaa
     8761 acaaaaacca aactgggaaa tagtgaggtg atcggcgctg tagatcaggc tactggagaa
     8821 gcattgaaca attacctgga ggagcaccca aaagaagcaa aaattattgt ggaaaaggtt
     8881 attcttgctg ctacggcacg ccacgctgca cgaaaggcac gcgagctgat tcagcggaaa
     8941 tcaccgttgt cgggtggagg acttcccgga aagttggccg actgttccga caaggatgct
     9001 gagaaatgcg aaattttcat cgtcgaagga gattctgcag ggggaacagc taaacagggc
     9061 cgagatcgta tgtttcaggc catccttccg ttacgtggaa aaatcctgaa cgtggagaag
     9121 gtgatgaccc accgggttta tgaaaatgaa gagatccgga acatatatac tgccttgggg
     9181 gtaactatcg gaacggaaga agattccaaa gcgttgaata tcgaaaaact ccggtaccat
     9241 aaggtggtaa tcatgaccga tgccgacgtg gacggaagcc acattgccac acttatcctg
     9301 actttctttt tccggtatat gaaaccgctt atcgagaacg gttacgtcta tattgccact
     9361 ccgccacttt atctgtgcaa gaaaggtaaa atcgaagagt attgctggga tgaccgtcaa
     9421 cgccaggcat ttatcgacaa atatgccgat ggaaatgaga acgcagttca ttctcaacga
     9481 tacaaagggt tgggagagat gaatgccgag cagctttggg aaacaacaat gaatcccgaa
     9541 aaacgtacgc tccgtcaggt aaccatcgaa aatgcagcgg atgccgatat aatcttctcc
     9601 atgctgatgg gtgaagatgt tgctccacgc cgggagttta tcgaagagaa tgctacttac
     9661 gcgcacattg acgcataaaa ctgggattgt atgctccaac ccccaatatt acaaaccggt
     9721 gataaggcag ttatcctttc acctgccggg aagatagatg ccgggctggt ggcaaaggca
     9781 gcagacttac tttgtgattg gggattgggg gtggagattt ccgaaaatgc attgtgcagg
     9841 accggacgtt tcagcggctt cgtggagcaa cggctagcag acctgcagcg agccatggac
     9901 gaccctgagg tgaagttgat tttctgttcg cgcggaggat acggaactgt acatctgttg
     9961 gataaactcg actttacagg tataagacag tacccgaaat gggttgtcgg ttatagcgat
    10021 attaccgttt tgcatgcaac gttgcagcaa catgggatta tgtctgtcca cgggcctatg
    10081 gccaagcatt ttgccgagga aggcccgggt gacatttctg ttcgtttcac caaagccata
    10141 cttgccggag aacccgtttt ctacgatatt cccgtcgcaa agtatgctta cctcaaccgg
    10201 aaaggcaaag cctccggaag gttatttggc ggaaaccttt cggttttttg cggtattcta
    10261 ggtacacgat ttgcctattt acccaaaaac ggcattttgt ttatcgagga tattggcgaa
    10321 gagccttaca agatcgatcg gtacatcaat caacttaaac ttgcaggtat cttcgaccgg
    10381 atatcgggat tgatcgtagg tcagttcagc gattataccg aagacaacga tatgtattca
    10441 gcgctttacg aatccatcgc atccgttgtg acgggatatg attttccggt ttgtttcgac
    10501 ttcccggcag gacacgtgaa gcacaacttc ccgcttgtga tgggaaagac ggcaaagctt
    10561 gttgtcaaag aggatcaact tatattcaaa caatcttaaa gcaaaaagat ggaggtttca
    10621 actgaactta tattgcttat tggttctttt ctttttttcg taagtatgct ggtgggaaaa
    10681 gccgggcata aattcggtgt tccggttttg ctactcttcc tgttggtagg aatgattttc
    10741 ggaggggatg gattcggact taatttcgag aacattcaaa tagcacaggc catcggaacc
    10801 gtttgtttga caattatcct tttttccgga ggcttggaca caaaattcag agaaataaaa
    10861 ccggttattc agcctggggt tgttttggct acgctgggcg tatttattac ggccattatt
    10921 acgggtattt ttatctggtg gctgtctgat caggtttaca caggattggg agtgggtttt
    10981 ttaacagcta tgttgctggc ttccacggtt tcttctaccg attctgcgtc cgttttcggc
    11041 attctccgtt ctaaaggact tatgctgaag aataatcttc gtccgttact tgagttggaa
    11101 agtgggagta acgaccctat ggcttatatg ctgacggtta cctttatcag tcttatcaat
    11161 tcgggcaacg accccaatta tgtgatagta gccgtcaata tcgttgttca gcttgtggtt
    11221 ggagcgttgc tgggatactt ccttgggaga ttttcggtag tcatcatcaa ccgtatccga
    11281 atggacaata cttcgctcta tccggtcttg ctccttatta ccggaatatt tatttttgca
    11341 gtgacctact acctgaaagg caacggatac ctggcggttt atatcgcggg gttggtcatc
    11401 gggaactcaa agtttgcaca taaacgcaca tcgatgagtt ttatggatgg atttgcctgg
    11461 atgagtcaga tcctcctgtt cctgacgctg ggattgttgg ttaatcccag tgaattggtg
    11521 ccggtgttga ttccgggcat tatcattgcc ctttttatga tctttattgc gcgtcccatc
    11581 acggtttacc tctgccttat tcctttcaaa agaatttcat ttcgcgacaa agcctacatt
    11641 tcatgggtgg gattacgcgg agccgtacct attatttttg ccatattgcc gttggcagca
    11701 ggagtgcccg gggcaagaac agtgtttaat atcgtctttg tgattaccat cgtttccttg
    11761 ttgtatcagg gtacttcact tcccatagtg gccaaatggc ttgggctggc ggaaaagccg
    11821 agcaagtaca aaagttttga ggatttcgat gttgagttct ccgaagaaat aaaatcggcc
    11881 atgactgaga tcagcattac tgaagagacg ctaaaacacg gcaagaacct tatggagatg
    11941 ccgttgcccg acaatacgct ggcggtgatg gtgaagagag gtgaacggtt ttttgtccct
    12001 accggacaaa cggctctcat ggatggagat aagctgttgg ttatttcgga cgatgaggag
    12061 gcgctgaaag aaacgtacaa gaacatcgga atttccaact tcacctatca gaaaaacgac
    12121 tgatggaaaa attaaactca ctgttcatcg tgttgctagt gggagttggc cttgtttttt
    12181 ctgtatcgtg caactcttgc cagtcacaga agaatgcagg taaagaaacg acagcaatcg
    12241 catatgcaaa agtatccccc gattttaatt ccgacagcgc ataccgtttt gttgaaaagc
    12301 aagtcagctt cggaccacgg gttccaggaa cgaccgcaca taaagcttgc ggtgactacc
    12361 tatcggaaaa actggctgcc ttcggtgcgg atatttttga acaaaaagcc cacgtaacgc
    12421 attataacgg acagaacatt cagatccgta acattatcgg gagttttcag cccgaaaaag
    12481 agaaacgtat tttattgttt gcccattggg acagtcgccc gtttgccgac gaggaaccgg
    12541 atcccgatac acaaaataga cctattcccg gtgcagacga cggagcaagt ggagtagggg
    12601 tgcttttgga gattgcccgg caactgcagc tgcagcctcc cgggatagga gttgacatca
    12661 tcttttttga tttggaagac tgggggcagc ccgcattcga taaaaattat ctttcgggtg
    12721 aatggtggtg tgtgggatcc aggtattggt cggaaaatcc acacaaaaac aattacaagg
    12781 cttcgtacgg aattttactc gatatggtgg gtgcagcaaa cgctacattc ttgcgcgaag
    12841 gctattcaat gcagtatgcg tcgaatgtcg tgtcaaagat atggagcatc gcttcccaca
    12901 tgggatacgg gaaatttttc gtacaacgga acggcagtta tatcaccgac gatcacgtgc
    12961 cggtaattga gaaacggggt attccgtgtg caaacatcat taatttaaag gatactgaca
    13021 atggctttgc gccacactgg cacacgcaca acgacgatat gcgcaacatc agtaaggcaa
    13081 cgctccaggc taccggacaa acggtgctgg aagtggttta ccgggaatta gacgaaaaca
    13141 acatatgaca atcaacgaaa cagaacaaga gattatcgat gaattcagca tctacgatga
    13201 ctggatggat aaatacgctt atatcattga gcagggaaat caactggcac cacttgacga
    13261 gaagtacaag gtgcccgaaa atattattca aggctgtcag agtcgtgttt ggctgcagac
    13321 tgactaccgg gacgggagat tgtacctcca ggcagaaagc gacgctgtaa ttgtgaaagg
    13381 cttgttagcg ttggtattgc gtgtgtttaa tcaccgtact cccgacgaaa gccttgcttc
    13441 cgacctccgc tttatgaagg aaatcggact tactgaacac ctttctccta cccggtcgaa
    13501 tgggttgctt tccgtgatca aacaaatccg gttttatgct atggcatata aagcaaagag
    13561 cgattcggaa aaaagatgac tcaaccctca aaacccaata tatgaaacaa ctcttattct
    13621 tattattatt attatttatg acgactatga ccaccgcttc aatggcggat gaaaatcctt
    13681 tttttaaacc gtacgatact ccatacggca cgccaccgtt cgataaaatc aagattgagc
    13741 attatgagcc ggctttcgat gaagctatcc ggcagcacaa ggttgaaatt gaaaccattg
    13801 ctgctaatcc gtttgcgccg acttttcaga ataccattgc tgcaatggaa tattcaggcg
    13861 aaatgttgaa ccgtgtgagc ggtgttttct tcaatttact gagtgctgaa agtaacgatg
    13921 agatgatgat gatttcgcag cggttgagtc cgaaattatc ggagcattcc aacaacatca
    13981 acttgaatga aaaattattt gcccgggtaa aaacagttta cgataaccgc ctgacttcgg
    14041 gtttactccc tgaacagatc cgtttggtgg aaaaatacta cgaacagttc gagaatagcg
    14101 gcgcaacact ttctgctgga gacaaggaaa cctaccggaa gctgtcgatg gagctgagca
    14161 aaacaaccct cgattttggg caaaacaacc tgaaagaaac taacaggttt gaaatgctgc
    14221 ttaccgatga agccgatctg gccggattgc ccgtgagcat tctggaggca gctgttgcca
    14281 aggcaaaatc gaaaggaaaa gaaggatgga tgttcgattt gtcggctccc agctacatcg
    14341 gttttatgaa atattctacc cgtcgtgacn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    14401 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    14461 nnnnnnnnnc gatttgtcgg ctcccagcta catcggtttt atgaaatatt ctacccgtcg
    14521 tgacttgcgc gagaagcttt acatggctta caacaccaaa agtgttatgg gtggagagtt
    14581 tgacaataaa gaaaacatta aaagaatcgt aaacttacgc ctgcagattg ccaatttgct
    14641 gggttaccgg aactacgctg cctacgccct gaaaaatagg atggcaaaga acgaggaagg
    14701 ggtttacagc ctgctcgaag agctggcccg ggcttacgga gaaacagctc gccgggaggt
    14761 gaaagaggtg gaggcctttg ctgtcaaaat ggaaggtaaa ccgattgaaa ttcagccgtg
    14821 ggactggagt tactattccg acaaactgaa agacgatcgt ttcgacctga atgatgagat
    14881 gacgcgtccc tactttgaac tcgaaaacgt gaagaaaggc gttttcggac tggcaaccga
    14941 cctttatgga atcactttcg tgaaaaatcc tagcacaccg gtctatcatc ccgaagtcga
    15001 ggcgttcgac gtgatggatg ccaacggcga ttttcttgct gtcctgttta ccgattttca
    15061 tccgagagag ggaaaacgtt cgggtgcctg gatgtcgtcc tttaagtcgc agttcgtgaa
    15121 gaacggagtg gatagccgtc cgcacatcac catcgtgatg aacttcaccc gccccaccga
    15181 aacaaagcca gcactgctca ctttcgacga agtggaaaca tttctgcacg agttcggaca
    15241 tgccttacac ggcatgttgg caaaatcgac gtatgaaacc ttgtcgggaa caagtgttta
    15301 tcgtgatttt gtggaacttc cgtcacaaat tatggaaaat tggttagtag aaaaagaata
    15361 cctcgataaa tttgcattcc attatcaaac gggtgaaaaa atgcctgcgc agttagtgca
    15421 gaaaatcatc gatgccgcca attacaatac cggctacttg acacttcgcc agctttcgtt
    15481 cggatacctg gatatggcgt ggcacacatt acagcaaccg tttgacggag atatccgtga
    15541 atttgaacaa aaagcgatgc gtccggtaca attgcttccg gttgtgcccg aaacaagcat
    15601 gtcgacagcg tttgggcata tattctccgg tggttatgct gctgggtatt atagttataa
    15661 atgggcggaa gtgcttgatg cagatgcttt cgcggccttt aaggaaaacg gaatctttga
    15721 taagaaaacg gctcagtcgt tccgggtaaa tatcctggaa agaggaaaca ccgaagagcc
    15781 aatgacactg tacaaacgct ttcgagggca cgaacctacc gttggtgccc tattagaaag
    15841 aaacggagtg aaacagtaaa atttaacctt cggcgaaaga gtaaatcgcc gaaggttaaa
    15901 ttttttttag tttttttgct aatttgtttt gtcacaacaa atttattcct acctttgttg
    15961 ctgcaaaagt tttttatagc agaaacgggt atttggttat tttcttcctt ctcaggggca
    16021 aatcaattct ttaaccttat cttttataca atatcttctg caccaattaa ttttttattt
    16081 attttatttt tatttttaca tgaacatttt tgttgcaggc ttgagttatc aaattaacga
    16141 cgccgattta aaagaactgt ttgaggaata cggagagatt acctcagcta aaattattac
    16201 tgacagagaa acacgcagat cgaaaggtta cggttttgtt gaaatggctg acgaagatgg
    16261 tcagcgcgct attgaagaat tgaacggagc tgaatacgac ggtcgtacac tgtctgtctc
    16321 agaggctcgt ccccgtgccg aaggagataa tcaacgcggc aatagaggcg gtggtaacag
    16381 aggcggttac ggaaatcgtg aaagaagata ttgatatttc tcaataggtc aaagaaaagg
    16441 agactgcgcg tttatgtgca gtcttttttt atgaaccatt gctatcatta agcgttatac
    16501 caggcagttt ataaactcac aacccctatg cctactatca tacatataga aacagctaca
    16561 gaagtttgtt catgtgcttt atcgtccgga ggaaaagttt tgctgaacag ggaaaacctg
    16621 aacggacaat cgcacgctac gtccttgggc gtttttgtgg aagaaatgat acagtttgtc
    16681 cgcgataacg gtatgaagat cgatgctgtg tcggtaagta gcggtcccgg ttcttatacc
    16741 ggattacgca tcggagtatc ggaagccaaa ggattgagct atggattgga agtccccctt
    16801 attgctattc ccacccacaa gattatggct tggatgatga gaacgggaat tgaacctgac
    16861 cacttgctct gccctatgat tgatgcccgg cgaatggagg tctatgctac catcttcaac
    16921 gcgaacttgg aggttattcg tgccacctcg gcagatatcg tggataaaga gagttatgcc
    16981 gagttactga aaaatcaaaa aatcctgttt tttggcaacg gtgccgaaaa atgtaaaggt
    17041 attatcctgc atccgaacgc ttcgtttatt ccggctgtaa aaccaaaggc agaagccatg
    17101 actgccttgg ctgaaaatgc gtttgccgcc gctgattttg ttgactctgc ctattttgag
    17161 cctttttacc tgaaagagtt tgttgctact gttcctaaga acaaaataat ctaaataccc
    17221 gtctgttttc ccttcctttc tttc
//
LOCUS       DAOSDR010000009        16991 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrimonas sp. isolate AMR_MDS_1826
            NODE_1371_length_16991_cov_4.245276, whole genome shotgun sequence.
ACCESSION   DAOSDR010000009 DAOSDR010000000
VERSION     DAOSDR010000009.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863355
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrimonas sp. (wastewater metagenome)
  ORGANISM  Petrimonas sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Dysgonomonadaceae; Petrimonas.
REFERENCE   1  (bases 1 to 16991)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16991)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:10:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,720
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 35
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16991
                     /organism="Petrimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1371_length_16991_cov_4.245276"
                     /isolate="AMR_MDS_1826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2023866"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..504)
                     /locus_tag="PLI30_00815"
     CDS             complement(<1..504)
                     /locus_tag="PLI30_00815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019541035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA degradosome polyphosphate kinase"
                     /protein_id="HOI78126.1"
                     /translation="MNNNSLYKYFKRDISWLSFNHRVLLEAKDESLPVYERIKFLSIY
                     SSNLEEFYKIRVSGYHSSILKNISRDESEEEALQTLMEINNEVTEQEKEYYRIFNEMI
                     LPELEKNNIILYQTDEVEPFHRAYVEKYFNEEVFPYLQPMIILKDDIHSFIQDGRIYQ
                     VVSLVKKK"
     gene            complement(541..903)
                     /locus_tag="PLI30_00820"
     CDS             complement(541..903)
                     /locus_tag="PLI30_00820"
                     /inference="COORDINATES: protein motif:HMM:TIGR02436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HOI78127.1"
                     /translation="MSNQEWINYKIEIRDRLRAFSLEILSLSEMLPEKTRGKVLNYQL
                     TKSGTSMYANYRAALRSRSKAEFFSKLTIAVEEADETEMWLDLIIASGILTTDYVKSL
                     HAESEELLKILAAMRKKL"
     gene            1050..1547
                     /locus_tag="PLI30_00825"
     CDS             1050..1547
                     /locus_tag="PLI30_00825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005935675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HOI78128.1"
                     /translation="MKKIGLLSDTHNVWDEKYENYFANCDEIWHAGDIGSLELAGKFE
                     SLKPFRAVYGNIDDFSTRRIYPETLRFTLEGVDVLMTHIGGYPGKYDPKIRSQLFSKP
                     PSLFICGHSHILKVIYDKELGCLHMNPGAAGKYGFHRVRTLLRFMLDNGDIRDLEVIE
                     IGKHG"
     gene            complement(1544..2179)
                     /locus_tag="PLI30_00830"
     CDS             complement(1544..2179)
                     /locus_tag="PLI30_00830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005826316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HOI78129.1"
                     /translation="MFETIVKGFIIGLLVSAPMGPVNMLCVQRTLNKGRWSGFITGLG
                     ALLSDLIYALITLLGMSLVEDFLKKNELLIQLTGSVVLIFFGYVVFRTNPLKGWTPQV
                     KIETKHHFRDFISSFFITLSNVFIIFVFITLYARFTFTPVTEGKGYLMLALVAISAGA
                     LIWWFFLSLFISRLRKHFNRKGLSILNKTIGLILIIISVVGIFMSIFGVAL"
     gene            2315..5998
                     /gene="purL"
                     /locus_tag="PLI30_00835"
                     /gene_synonym="purI"
     CDS             2315..5998
                     /gene="purL"
                     /locus_tag="PLI30_00835"
                     /gene_synonym="purI"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005644489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HOI78130.1"
                     /translation="MITFFRTPSQSIIAVDAIHPIQNELQERLIWLFGKAEPIPFFEI
                     NEKFTGPRREMITPWSTCAVEITQNMGITGITRIEEYFPAGEVPEYDPMLQRIYKKLD
                     QDIFTTDKQPESIVLIDDIEAYNQQEGLALNEDEIDYLNNVSRKMGRKLTDSEVFGFS
                     QVNSEHCRHKIFNGKFVIDGEEKESSLFQLIKKTSGVNPNALISAYKDNVAFVQGPTI
                     EQFAPASGDKPDFFETREIESVLSLKAETHNFPTTVEPFNGASTGTGGEIRDRLAGGK
                     ASLPSAGTAVYMTSYPRMEAGREWEKILPERRWLYQTPEQILIKASNGASDFGNKYGQ
                     PLICGSLLTFEHAENYKKFGYDKVIMLAGGVGFANKLNALKGNPQPGEKVIVMGGDNY
                     RIGMGGGAVSSVNTGEYSSGIELNAVQRANPEMQKRVANVIRTLAESEDNPIVSIHDH
                     GAGGHLNCLSELVEKTGGKIEMEKLPVGDPTLSAKEIIGNESQERMGLLVPEKDIDRI
                     RQIAERERAPFYVVGETTGDMRLTFQQADGKMPIDMEMEDFFGKSPKTVMSDATVEET
                     YTSPDYDEKDLKTYIENVLRLEAVACKDWLTNKVDRSVTGKVARQQCQGEIQLPLSDC
                     GAVALDYRGYAGVATSIGHAPQVALIDPAAGSVVAIAEALTNIVLAPLKDGLQSVSLS
                     ANWMWPCRNEGEDARLYKAVEACSDFACDLGINIPTGKDSLSMTQKYGDDKVFSPGTV
                     IISAAAEVKNIRKIVSPALAYIKGTYVYYVDFSFDALRLGGSAFLQTLGKVGDEAPTV
                     SDSEYFKNAFAAVQELVNRGLIIAGHDISAGGMVTAMLEMCFSNPQGGLDARLDKIRH
                     SDLIKILFAENPGVLIQVKHHHLVEKILNDYGIGFAIVARPIEERRLIIEKGEFKQEF
                     DIDVLRDVWYRTSYLFDKKQSGEECALDRFKNYKKQPLQFNFLPSFTGKMQDLDLNPD
                     RKERSGLTAAIIRDKGTNGEREMAYALFLAGFDVKDVHMTDLTSGRETLEDVQFAVFC
                     GGFSNSDVFGSAKGWAGGILYNGKARKTIENFYARPDTLSLGICNGCQLLMELGLIYP
                     EAGKAHPKMQHNRSHKFESAFLSVEIPQNSSVMLKSLAGTKLGIWVAHGEGRFELPGQ
                     ESAYNIAAKYVYDEYPGNPNGSDYKAAAVCSADGRHLAMMPHLERTIFPWQNAWYPYE
                     FRKHDVTPWMEAFVNAREWLKKK"
     gene            6095..8479
                     /locus_tag="PLI30_00840"
     CDS             6095..8479
                     /locus_tag="PLI30_00840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009433755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOI78131.1"
                     /translation="MKRLVIVFSFFFIGSVLFAQKGIIKGRILNAKTNEPIEFANIQI
                     QGTSIGSTSDLDGNFIFTGIDPGFVRLAASFIGFETTVSSEIQVQGNQTTFIDIELPE
                     TAISLEEVVVRPNFSLKRIESPVSILTIGVQEIEKSAGVNRDVSKAMQTLPGVGATDP
                     NRNDLIVRGGGPSENVFYLDGIEIPIINHFSTQGSSGGVVGVVNPDFVREISFYTGAF
                     PANRTNALSSVMEIRQKEGSRDRVHTKLSVGASDAAITVDGPAGTNSTFIASARQSYL
                     QLLFKAIGLPFLPTYNDFQMRYKYNINAKNELTFIGIGAIDNMTLNTGLQESGTESQR
                     YLLGYLPVYNQWNYTVGTVFKHFGSRFFDTWALSRNMLRNKNFKYLDNDESKPKTLDY
                     LSDEAENKLRFERVYTGLPVKLMLGGGIEYARYTNSTLAKVFLNGTVNDINYHTQLGL
                     FGYQAFAQVSDEYLKNRLKLSLGISTVGNNFNDNMKDPLNQLSPRFSASYVLTDKWDL
                     NANFGRYAMQPAYTSLGFKNAAGEYANRNEGVKYIFSNQAILGVEFRPKNNVRMTLEG
                     FYKQYENYPLSVSDGSSLASKGTEYGQVGDEEVLSAGKGRAYGVEFFSKILEWNNLNV
                     TTTYTFFRSEFTDKEGVYRPSSWDTKHLLNLIGSYKFPKNWNVSARWRFVGGAPYTPV
                     DMDLSTNKAAWNITNRAYIDYSNFNSLRLPNAHQLDMRIDKEIYYKKWTLNLYADVQN
                     VYNFKSRNAPIYTNKDVDGAVMDDPADPENRQRIRIIDAYSGTVLPTVGIIIKM"
     gene            8500..9246
                     /locus_tag="PLI30_00845"
     CDS             8500..9246
                     /locus_tag="PLI30_00845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI78132.1"
                     /translation="MNPTRNILLLLVAFFAITLSSCVSVKKEPGQVQEFTYGKRNGGS
                     LITLKFEKGKEHNHPLFAIWLADSEGNFLQTLYVSESIGTGVFKHVSREAGRWMAGEI
                     QRPAALPYWAYQRNIRNEAGTLLPTPQQPVPDAYTGATPSSSFVFYLNTDKPLNGVYT
                     LFLELNQSWDWNEHWTNNKFPGDNEYKTSSQPALVYVTKLDTNSREEMELKPVGHSHY
                     SGKDGKLYDNLNTLSTALKIANKITAVVKP"
     gene            9348..9749
                     /locus_tag="PLI30_00850"
     CDS             9348..9749
                     /locus_tag="PLI30_00850"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019541031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HOI78133.1"
                     /translation="MDPIFTYTMKVRDYECDTQGHVNNANYQHYFEVTRHEFLEKVGL
                     NFYQLHKQGIDAVVSRVEIRYKVPLIGMDRFICTVSRLERIGLKYIFHQEIVRQSDNV
                     LCARAKIEVVNLVNGKLSQPEVFDEAFKEYI"
     gene            9803..10909
                     /gene="dprA"
                     /locus_tag="PLI30_00855"
     CDS             9803..10909
                     /gene="dprA"
                     /locus_tag="PLI30_00855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005945382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HOI78134.1"
                     /translation="MTDKTLYQIALTCIQGVGVVHARNLMRAVGDEKAIFSEKTGSLE
                     KLPGLSRRLIHEIRNPEVLRKAEKELAFVEKNNIQTLFYTQHHYPSRLTNCVDAPVLL
                     YAKGNTGFNAPKVVSIIGTRNATRYGLEFCQKFVRELSGLFPDILVVSGLAYGIDICA
                     HRASLQNNVSTVAVLAHGLDRVYPSVHRKTAIEMLERGAWITEFPSGTEPDRFNFVKR
                     NRIVAGMADAVVVVESGDRGGSLITADIANSYYREVFAIPGRITDARSSGCNNLIASN
                     KAVLMQNTVAFIEQMGWDTAEKPEQPVQKELSPDLNEVEEKVFRALNGVESMHVNKLG
                     IELNLPVSELFFTLLELEMKNVVKALPGGMYKLV"
     gene            11000..11458
                     /gene="bcp"
                     /locus_tag="PLI30_00860"
     CDS             11000..11458
                     /gene="bcp"
                     /locus_tag="PLI30_00860"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008158590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="HOI78135.1"
                     /translation="MALQIGDKIPEILGTGQDGKTVKSTDFTGKKLALYFYPKDNTPG
                     CTAQACSLRDGYQDLKKAGYAVVGVSVDSEDSHRKFIEKFDLPFPLIADVDKKLVQEF
                     GVWQEKKNYGKTYMGTVRTTFLIDEAGIIRHIIEGRGVDTKNHAEQILNL"
     gene            11524..12537
                     /gene="recA"
                     /locus_tag="PLI30_00865"
     CDS             11524..12537
                     /gene="recA"
                     /locus_tag="PLI30_00865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019541028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HOI78136.1"
                     /translation="MAEKETPQNSEKLKALRAAMDKIEKTYGKGSIMKMGEEKVEEVS
                     VIPTGSIGLNAALGVGGYPRGRVIEIYGPESSGKTTLAIHAIAEAQKAGGVAAFIDAE
                     HAFDRFYAEKLGVDTDNLLISQPSSGEEALEIAEQLIRSSAVDIVVIDSVAALTPKKE
                     IEGDMGDSNVGLQARLMSQALRKLTSTIGKTNTTCVFINQLREKIGVMFGNPETTTGG
                     NALKFYASVRLDIRRIGQLKDGDEVHGSQTRVKVVKNKVAPPFRKAEFDIMYGEGISR
                     EGEIVDLGAELNVIKKSGSWYSYNDSKLAQGRDATKAVIKDNPELADELEKLIFEALK
                     EKK"
     gene            12618..12803
                     /locus_tag="PLI30_00870"
     CDS             12618..12803
                     /locus_tag="PLI30_00870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019539593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI78137.1"
                     /translation="MGRKTQGSGSAGDAVYGLGVIGAAVWYISTAATFWMGVLGFLKA
                     IVWPAFLVYEAFKALLG"
     gene            12889..14877
                     /locus_tag="PLI30_00875"
     CDS             12889..14877
                     /locus_tag="PLI30_00875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019541027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4954 family protein"
                     /protein_id="HOI78138.1"
                     /translation="MQELRSLTPQEIERLQSQNCECADWTRVKVSAGFNPHFIKNTRF
                     SGKIKLGVLENEFELAGGLKKHSCINNAVIHNCEIGNNVVIENIQNYIANYLIGDNCF
                     IQNVDVILVDAKTTFGNGTEVNVLNESGGREVHIHDKLSAHFAYVYALYRHRPNLIKR
                     MKAMVDYYAGKHASDMGSIGNDAMIVNVGYIKNVRIGSFCKITGAMKLKNGSINSNKQ
                     APVYVGRNVIAEDFIISSGSRIDGGCIISRCFIGQACHLDHGYSASDSLFFSNCQGEN
                     GEACALFAGPYTVTHHKSTLLIAGMFSFMNAGSGSNQSNHMYKLGPIHQGIVERGAKT
                     TSNSYVLWPAKVGPFSLILGRHYHHSDTSNLPFSYLVENNNATHLAPAINLKSVGTIR
                     DAKKWPERDKRKDPDKLDFINFNLLSPYTIQKVFAGVSILKNLQATAGETSEIYTYQS
                     CIIKNAALKRGLELYEIVIHKFLGNSIIKRLENTRFTSNEEVRDRLKPDTTVGSGEWI
                     DISGLIAPKSEIDHLLCQIESGEVSKLKEINKVFKQLHEQYYTLEWTWAWEKIQEFYS
                     VTPETITAEDVIAIVEKWKTSVVKLDEMIYTDAKKEFSLSFKTGFGSDGNIQDQAMDF
                     EYVRGAFDKNLFVMATLKHIEDKKALGDELIERIKNVK"
     gene            15153..15974
                     /locus_tag="PLI30_00880"
     CDS             15153..15974
                     /locus_tag="PLI30_00880"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009001 - serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HOI78139.1"
                     /translation="MIKKTFELINKFQPLPVQIPIDKCKLEEVVKTLFSQMFPVCERI
                     DMGDIHEKLKDCARELSVNIARLKDQQFADEAVYKFFSRFPEIQDLLYEDAKCYLKND
                     PAAKSLEEVIITYPGFFALSVYRMAHELHVLGVPLIPRLFGEYAHSKVGIDIHPAAKI
                     GKNLFLDHGTGIVIGETAEIGDNVKIYQGVTLGALYVEKKLSDVKRHPTVEDNVVIYA
                     NATLLGGETVIGHDSTIGGGAWLTQSVIPYSLVYNSVDVKIRTVKNFVQPLDFII"
     gene            16019..16927
                     /gene="cysK"
                     /locus_tag="PLI30_00885"
     CDS             16019..16927
                     /gene="cysK"
                     /locus_tag="PLI30_00885"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HOI78140.1"
                     /translation="MRFNNILESIGNTPHVRLSKLFPHHEVWIKLERQNPVGSIKDRI
                     ALAMIEDAEKKGILKPGGTIIEPTSGNTGVGLSFVGAYKGYKVIIVMPESMSVERRRL
                     IGLFGSELVLTPREKGMKGAIEKALELQSGIPGSWIPQQFQNQANVTVHAEKTAKEVL
                     DDFPEGFDYLITGVGTGGHITGVGRELKKKFPSIQVIAVEPAASPVISGDSPGPHTIQ
                     GIGAGFIPDTLDLNVLDSVIKVSNEDAFIYARRLAREESILGGISTGAAIAAVAQKVS
                     QLTEKKRILTFNYDTGERYLSVDGLY"
BASE COUNT         4898 a         3563 c         4123 g         4407 t
ORIGIN      
        1 ttttttcttc accaggctta ccacctgata gatacgccca tcctggatga aagagtggat
       61 atcgtctttc aggataatca tcggttgcag gtaaggaaac acctcttcgt taaagtactt
      121 ctccacgtag gcacggtgga agggctccac ctcatcggtc tgatataaaa tgatattgtt
      181 cttttccagt tcgggaagaa tcatctcgtt gaatatgcgg taatattctt tttcctgctc
      241 cgtgacttcg ttattgatct ccataagtgt ttgcagtgct tcttcttccg attcgtcacg
      301 gctgatgttt ttcaatattg aactgtggta tccggatacg cggattttgt aaaactcttc
      361 caggttggaa gagtagatcg atagaaattt aatccgctcg taaaccggaa gcgattcatc
      421 ctttgcttcc agcaagacac ggtggttaaa cgacagccag cttatgtcgc gcttaaagta
      481 tttgtagagt gaattgttgt tcattttttt agattgaaaa tagattgaat gagattgaat
      541 ttataatttt tttctcattg cagctaaaat tttcagcaat tcttcgctct ccgcatgtaa
      601 cgattttaca taatcggttg tgagtattcc cgatgcgatt atcaagtcga gccacatttc
      661 cgtttcatca gcttcttcaa ctgctatggt taatttactg aaaaattctg ctttagatct
      721 gcttcgcaat gctgccctat agtttgcgta cattgacgtt cccgattttg tgagttgata
      781 attgagcact ttgccgcgtg ttttctcagg caacatttcg gacaaagata aaatctcaag
      841 ggaaaaagcc ctcaatctgt ctcttatttc tattttgtaa tttatccact cttgattact
      901 catagtcgga ttttcaagtt ctgatataca agaagcaaag ccaagatata actgtatttg
      961 gcgcttttca atctttttaa ttaaacaata aaattagtaa ctttgttgca ataaaaaaag
     1021 aataaatggg ctacatcaat cttaactgaa tgaaaaaaat aggcttactt tccgacacac
     1081 ataatgtctg ggacgaaaaa tacgaaaatt attttgccaa ttgcgatgaa atctggcatg
     1141 cgggagatat cggttccttg gagttagccg gaaaatttga gagtctcaaa ccgttcaggg
     1201 cggtttacgg aaatatcgac gatttttcta cacggagaat atatcctgaa acgttacggt
     1261 ttacgttgga aggtgtagat gtccttatga cacatatcgg aggatatccc ggaaaatatg
     1321 acccgaaaat ccggtcacaa cttttttcaa aaccccctag cctttttatt tgtggacatt
     1381 ctcatatact aaaagtcatt tacgataagg agttaggttg tttacacatg aatccgggtg
     1441 cagcgggaaa atatggtttt catcgggtga gaacgctgtt gcggtttatg ttagacaatg
     1501 gagatatcag ggatctggaa gttatcgaga taggaaaaca cggctagagt gcaacgccga
     1561 atatcgacat gaaaattccg acaacactga taattatcaa aattaaccca atcgttttgt
     1621 tcagaatgga taatcccttt cggttaaaat gcttacgcag ccgtgaaata aacaaactaa
     1681 gaaagaacca ccatatcaat gctcccgcgg aaattgccac aagtgccagc atcaggtagc
     1741 ctttcccttc ggttacggga gtaaacgtga accgggcgta cagggtgatg aaaacgaaaa
     1801 taataaacac gttggacagg gtgatgaaga aggaggagat aaaatcgcga aaatggtgtt
     1861 tcgtttcgat cttgacctgg ggtgtccatc ctttcagggg attcgtacgg aaaaccacat
     1921 agccgaagaa aataagtacc acactgccgg tcaattgaat cagcaactcg ttttttttca
     1981 agaaatcttc cacaagactc attccaagca acgtgataag agcatagatc aagtcggaaa
     2041 gcagagcgcc caaaccggtg atgaatccgg accacctgcc cttgtttagc gttcgctgta
     2101 cgcacagcat attcactggt cccatcggcg ctgagaccaa caggccgata ataaatcctt
     2161 ttacaatagt ttcaaacatt tgactgcacc gaagaaatta tggtttttac agtcgcaaag
     2221 atacctccat tcttaaaacc tacaaaaaaa gattttgtct ccacttttac gcaggttttg
     2281 attaactttg caactaaata gaagataaac tcatatgatc acttttttca gaactccgtc
     2341 gcaaagcatc attgccgttg atgcaataca tcccattcag aatgaacttc aagaacgatt
     2401 gatttggctg ttcggtaagg cggaaccgat tccctttttc gagataaacg aaaaatttac
     2461 cggtccgcga cgcgagatga tcacaccctg gagtacatgt gctgtggaga ttactcaaaa
     2521 tatgggaatt accggcatta cccggataga agaatatttt ccggccggtg aagttccgga
     2581 atacgatcca atgcttcaac ggatttataa aaagcttgat caggatattt ttactaccga
     2641 taaacaaccg gaatccattg tcctgatcga tgatattgag gcttataatc agcaagaggg
     2701 gcttgcattg aacgaagacg aaattgatta cctcaacaat gtgagccgga aaatgggcag
     2761 gaaacttacc gatagtgaag ttttcggttt ttcgcaggta aactcggagc attgccgcca
     2821 caaaatattt aacggaaaat ttgtgattga tggagaagaa aaagaaagtt ctctttttca
     2881 attgataaaa aaaacatcgg gcgtaaatcc caacgcgttg atatcggcat ataaagataa
     2941 cgtggctttc gttcaggggc caaccatcga acagtttgcg ccggcaagcg gtgataaacc
     3001 tgacttcttc gaaacccgtg aaatagaaag tgtactttct ttgaaagcgg agacacacaa
     3061 ctttcccaca accgtagagc ctttcaacgg ggcttctacc ggaacaggag gggaaattcg
     3121 tgatcgcctt gcggggggta aggcttcgct tccttctgca ggaacagcgg tctacatgac
     3181 ctcctatccc cgtatggaag ccgggcgtga atgggaaaag atattgccgg agcgcaggtg
     3241 gctttatcaa acgccggagc agatcctgat caaagcctcg aacggggctt ccgatttcgg
     3301 gaataaatac ggccagccgc ttatttgcgg ttcattactc acgtttgaac atgccgagaa
     3361 ttacaagaaa ttcggttacg ataaagtaat catgctggcc ggaggtgttg gctttgccaa
     3421 taagctcaac gcgttaaaag gcaatccaca gcccggggag aaagtgattg tgatgggagg
     3481 ggataattac cggatcggaa tgggcggtgg agccgtttcg tcggtaaaca ccggtgaata
     3541 ttcttcggga attgaactga atgccgtaca gcgtgcgaac ccagaaatgc agaaacgtgt
     3601 ggccaatgtc attcgtacgt tggctgaatc ggaagataat cccatcgttt ccatccacga
     3661 ccacggtgca ggcgggcatt taaactgctt gtcggaactg gtggaaaaaa ccggtggaaa
     3721 aattgaaatg gagaagttac cagtaggtga tccaactctt tccgcaaaag agattatcgg
     3781 taacgaaagc caggagagaa tgggtttgtt ggtccctgaa aaagacatcg accgcatcag
     3841 gcagattgct gaacgggaac gggcaccttt ctacgtggtg ggtgaaacta caggagatat
     3901 gaggctgaca ttccagcaag ccgatgggaa aatgcccatt gatatggaga tggaagattt
     3961 cttcggaaaa tcaccaaaaa cggtaatgag cgacgctact gtggaagaga cctatacttc
     4021 ccccgactac gatgagaaag acctgaagac atatatagag aatgtgctca ggctggaagc
     4081 tgttgcctgc aaagactggc ttaccaataa agtggaccgg tcggtgaccg ggaaagtggc
     4141 gcgtcagcaa tgtcagggag agattcagct tccgctgagc gattgcggtg cagttgcgct
     4201 tgattacaga ggatatgccg gagtagcgac ttccatcgga catgctccgc aggtagcctt
     4261 gattgacccg gctgccgggt cggtagtggc aatagccgag gccctgacta acatcgtgct
     4321 cgcaccacta aaagatggat tacaaagcgt ttcgttaagc gctaactgga tgtggccgtg
     4381 ccgtaacgaa ggagaggatg cccggttgta taaggccgtt gaagcttgtt ccgattttgc
     4441 ctgcgacttg ggaataaaca ttccaacggg aaaagattcg ctctccatga cgcagaaata
     4501 cggtgacgac aaggtgttct caccgggaac agtaattatt tccgcggctg cagaagtgaa
     4561 aaacatccgt aagatcgttt ctcctgctct ggcgtatata aaaggcacgt acgtttatta
     4621 tgttgatttt tcgttcgatg cgttacgctt gggcggttca gcttttttgc agacactggg
     4681 taaagttggc gatgaagctc ctaccgtgag tgatagcgaa tatttcaaaa atgcttttgc
     4741 tgctgtgcag gagctggtta atcgcggatt gattatcgcg ggacatgaca tctctgctgg
     4801 ggggatggtt accgccatgc tcgagatgtg tttttccaac ccgcagggag ggttggatgc
     4861 gcgattggac aagatccggc attccgacct gattaagatt ctctttgccg aaaatccggg
     4921 tgtccttatt caggtgaaac accatcacct ggtagagaaa atcctgaatg attacggcat
     4981 tggttttgct atcgttgccc gccctatcga agagcgcagg ctgatcatcg aaaaaggaga
     5041 attcaaacag gagtttgata tcgatgtcct tcgggacgta tggtatcgca cctcctacct
     5101 gttcgacaaa aaacagagcg gcgaggaatg cgccctggac cggttcaaaa attacaaaaa
     5161 acagccgctg caatttaatt tcctgccttc atttaccggg aaaatgcaag acctggattt
     5221 gaatcccgac aggaaagagc gttcgggact gaccgcagcc atcatccgcg acaagggaac
     5281 caacggagaa cgtgaaatgg catacgcact ctttctcgcc ggatttgatg tgaaagacgt
     5341 gcacatgacc gatttgacat cgggacgcga aacactggaa gacgtgcaat ttgccgtttt
     5401 ctgcggcggg ttttccaatt cagacgtttt tggctcggca aaaggttggg cgggtggtat
     5461 cctctataac gggaaagcca gaaaaaccat tgaaaatttc tatgcgcgcc ccgatacgtt
     5521 aagcctgggc atttgcaacg gctgccagtt attgatggag ttgggtttga tttatcccga
     5581 agcggggaaa gcccatccga aaatgcagca caacaggtcg cacaaatttg aatcggcatt
     5641 ccttagcgtg gaaattcccc aaaactcctc ggtgatgctg aaatcactcg ccggaaccaa
     5701 acttggtatc tgggtggcac acggcgaagg acgtttcgag ttgcctggac aggagtctgc
     5761 ctacaacatc gcggcgaaat acgtctacga cgagtatccc ggaaatccca acggttccga
     5821 ttataaagcc gctgctgttt gttcggctga tggccgccac ttggccatga tgccgcactt
     5881 ggagcgcacc atttttccgt ggcagaatgc ctggtatcct tatgaattcc gtaagcacga
     5941 cgttacaccc tggatggagg cgtttgtcaa tgcacgggag tggctgaaaa aaaaatagag
     6001 cttgggggag gcctggagaa aacaaaaatc ttgttttttt gttaatagtg gcatatttat
     6061 tgccatgtat ttataattag tataaataaa atttatgaaa agacttgtta ttgtgttttc
     6121 tttttttttt atcggatcgg ttctctttgc ccagaaaggt atcatcaaag ggcgtatttt
     6181 aaatgcgaaa acaaacgagc cgattgaatt tgccaacatt cagatacaag ggaccagtat
     6241 cgggtctact tcggatttag atggaaactt tattttcacg ggtatcgacc cgggttttgt
     6301 ccgtttagcg gcaagtttta tcggttttga aacgaccgta tcttcagaaa ttcaagtcca
     6361 gggaaatcaa accacattta ttgatattga gttgcccgaa acggctattt cactcgaaga
     6421 agttgtggtt cgccccaact ttagcctcaa acggatagag agtcccgttt caatattgac
     6481 catcggagtg caggagattg aaaaaagtgc cggcgtgaac cgtgatgtgt caaaagcgat
     6541 gcaaacactt cccggtgtgg gggctacgga ccctaaccgg aacgatttga tcgtgagagg
     6601 tggtggccct tcagaaaatg ttttttacct cgatgggatc gagatcccaa taatcaacca
     6661 cttttccacg caaggctcgt ccggtggagt tgtcggcgtg gttaaccccg attttgtccg
     6721 ggaaatcagt ttttataccg gtgcctttcc tgctaaccgc acgaatgcgc tcagctccgt
     6781 catggaaatc aggcaaaaag aaggcagccg ggaccgtgtg catacgaagc tctcggtcgg
     6841 cgcatctgat gcggctatta ctgttgacgg ccctgccggg acaaattcaa cgtttatcgc
     6901 atcagcccgg caatcttacc ttcagttgtt gttcaaagct atcggactgc cgttcctgcc
     6961 tacctacaat gactttcaga tgagatacaa gtacaacatt aacgcgaaga acgagttgac
     7021 cttcatcggt atcggtgcca tagacaacat gacgttgaat accggattgc aggaatccgg
     7081 aacggaatcc cagcgctacc tgttgggtta ccttcctgtc tacaaccaat ggaattatac
     7141 ggttggaaca gtattcaagc atttcggaag ccgctttttc gatacgtggg cattgagccg
     7201 gaatatgctt cggaacaaaa actttaaata cctcgataac gatgaaagca aacccaaaac
     7261 actcgattat ctctccgacg aagccgaaaa taaactgcgt tttgaacggg tttatacggg
     7321 tttacccgtg aaacttatgc tggggggagg tatcgagtac gctcgttata ccaattcaac
     7381 tttagctaaa gtctttctga acggcacggt taatgatata aattaccaca ctcaactcgg
     7441 cttattcgga tatcaggcat ttgcacaagt ttcggacgaa taccttaaga accgcctgaa
     7501 attgtcgctc gggataagca cggtaggcaa caacttcaac gacaacatga aggatcccct
     7561 gaaccagttg tcgcctcgat tttcggcttc gtacgtgctt acggacaagt gggatttgaa
     7621 tgccaacttc ggacgttacg ctatgcagcc ggcttatacg tcgctcgggt ttaaaaatgc
     7681 agcaggagaa tacgccaatc gcaacgaagg ggtgaaatac atcttttcca accaggcgat
     7741 tttaggcgtg gagttccgtc cgaagaacaa cgtaagaatg acgctcgaag ggttttacaa
     7801 acagtacgaa aattatccgt tgtcggtttc cgatgggagt agcctggcaa gcaaaggaac
     7861 ggaatacggc caggtgggcg acgaagaagt cttgtctgcc ggtaagggac gtgcctacgg
     7921 cgttgagttt ttcagcaaga tattggaatg gaacaacctg aacgtaacta caacatacac
     7981 ttttttccgc agtgagttta ctgataagga aggggtttac aggccttcgt cgtgggatac
     8041 gaagcacttg cttaacctca ttggcagtta taaatttccg aaaaactgga acgtgtctgc
     8101 ccgttggcgt tttgtgggcg gtgcacccta tacccccgtg gatatggatt tatccacaaa
     8161 taaagcggcc tggaacatta ccaaccgggc atatatcgat tacagtaact tcaattcgtt
     8221 gcgccttccc aacgcgcatc agctggacat gcgtatagat aaggagatct attacaagaa
     8281 atggacgctg aacctctatg cggacgtgca gaacgtctat aactttaaat cgagaaatgc
     8341 gccgatttat accaacaagg atgttgacgg tgccgttatg gatgatccgg ccgatcctga
     8401 aaaccgtcag cgaatccgta taattgacgc ctatagtggc accgtattgc ctacggtagg
     8461 gataatcatt aaaatgtaaa ctctaaacac ggtaaaacga tgaatccaac aagaaatatt
     8521 cttctgttgc ttgtggcatt tttcgccata actctttctt cctgcgtttc ggtaaaaaaa
     8581 gaacccggac aagttcagga attcacatac gggaaaagaa acgggggaag ccttatcacg
     8641 ttaaaatttg agaaaggaaa agaacataat cacccgcttt ttgctatctg gttagccgat
     8701 tccgaaggta attttttgca aactctttat gtgtccgaaa gcatcgggac tggggtattc
     8761 aaacatgtga gccgcgaagc cggacgatgg atggcgggcg agattcagcg accggcagcc
     8821 cttccttatt gggcttatca acgaaacata agaaatgaag ccggaacgtt gttgccgact
     8881 cctcaacagc ctgtccctga tgcttataca ggagctacgc cgtcgtcttc tttcgttttt
     8941 tatctcaaca ccgacaaacc gttgaacggg gtttatacgt tgtttctgga attgaaccaa
     9001 tcgtgggact ggaacgaaca ctggacaaac aacaaatttc ccggcgacaa tgaatacaag
     9061 acctcgagcc agccggcatt ggtttatgtg actaaattag acacaaatag ccgggaagag
     9121 atggagttaa agccggttgg acacagccat tactcgggca aggacggaaa attatacgat
     9181 aacctgaata cattgtccac cgctctaaaa attgcaaaca aaattactgc tgtcgttaaa
     9241 ccttgatgag gatttgcttt taaaggattt ttgcccgata ttgccttgat gcatatactg
     9301 gaatttcgta cttttgtaat tattacttaa cccgaagtgc gttttctatg gatcctattt
     9361 ttacatatac aatgaaggtg cgtgattatg agtgtgatac gcagggccac gtcaataatg
     9421 ccaactatca gcattatttt gaagtaacac gccatgagtt tctggagaag gtgggattga
     9481 atttttacca acttcataaa caaggaattg atgctgttgt gtctcgtgtg gaaatccgat
     9541 acaaagttcc gttaatcgga atggacaggt ttatttgtac ggtttcccgg ctggagagga
     9601 tagggttgaa atatattttt catcaggaga ttgtccgtca atccgataat gtgctttgtg
     9661 cccgtgcaaa gatcgaagtg gtcaacctgg tgaacggaaa actttcacaa cccgaggtct
     9721 ttgacgaagc gtttaaggag tatatttaga gtaaggaaca agggacaaag agatattcta
     9781 aactttaaat ctcaatctca tgataaccga caaaacccta tatcagattg cgttaacctg
     9841 tattcagggc gtcggagtcg tacatgcacg taacctgatg agggctgttg gggatgagaa
     9901 agccatattt agtgagaaaa cagggagttt ggagaaactg ccgggtttat cgcgacgttt
     9961 gatccatgag atccgtaatc cggaagtatt gagaaaagct gaaaaagaac tggcatttgt
    10021 tgagaaaaac aatattcaga cactttttta tacgcaacac cattatccct cccggttaac
    10081 taactgtgtt gatgcgcctg ttctgctgta tgcaaaaggc aacaccggct ttaatgcgcc
    10141 aaaagtggtg agcataatcg ggacgcgtaa tgcaaccaga tacggattgg agttttgtca
    10201 aaaatttgtc cgggaattgt cagggctttt ccccgatata cttgttgtga gtggcctggc
    10261 atacggaata gacatctgtg cgcatcgcgc ttcgttgcaa aataatgttt caacggttgc
    10321 ggtactggca cacggattgg atagagttta tccgtctgta caccggaaaa cggccataga
    10381 gatgttggaa aggggagcct ggattaccga atttccgagc ggcaccgaac ccgatagatt
    10441 caatttcgta aaacggaacc gcatcgttgc cggaatggca gatgctgtgg ttgttgtaga
    10501 atcgggagat agaggaggtt cactcattac tgcagatatc gccaattcct attatcgcga
    10561 agtttttgcc atacccggaa gaataaccga tgcccgatca tcgggatgca ataatttaat
    10621 cgcttcaaac aaagccgtat tgatgcaaaa tacagtcgcc ttcatcgaac agatggggtg
    10681 ggacacagcc gaaaaaccgg aacaacccgt gcaaaaagag ttgtccccgg acctgaacga
    10741 agtggaagaa aaagtttttc gtgcgctcaa cggggttgaa tccatgcacg tgaacaagct
    10801 gggaatcgaa ctcaacttac cggtttcgga gttgttcttt acattgcttg agctcgaaat
    10861 gaaaaatgtt gtaaaagcct tgccgggagg aatgtataaa ttggtgtgaa tttttcccgt
    10921 atcctattgc ggaagggaat tctttcttat ttaaaaaaac actatctttg atctctctac
    10981 cacaaaaaaa agaaaaatta tggcactaca gattggagac aaaattcccg aaatattggg
    11041 taccggtcag gacgggaaaa cagtgaaatc aactgatttt acaggaaaga aactggcact
    11101 ttatttctat ccgaaagata acacgcctgg atgtacggct caggcctgca gcctgcggga
    11161 cggatatcag gacctgaaaa aagccggtta tgcagttgta ggtgtgagtg tagacagtga
    11221 ggattcgcat cggaaattca tcgaaaagtt tgacttgccc tttccgttga ttgccgatgt
    11281 ggataaaaag ctggttcagg aatttggtgt ttggcaggaa aagaaaaatt acggcaaaac
    11341 ctatatgggt acagtccgca ccacattttt aattgacgaa gccggtatta ttcgccatat
    11401 cattgagggt cgaggtgtgg atacaaaaaa ccatgcagag cagatattga atctgtgaga
    11461 ggaaaatctt atgcttcgct ttgagcggct gacaagaaac aaataataaa aaacgaaaat
    11521 attatggcag aaaaagaaac cccacaaaac agtgagaaac tcaaagcgct tcgtgcggcg
    11581 atggataaga ttgagaaaac ctacggaaag ggttcgatca tgaagatggg cgaagaaaaa
    11641 gtagaggaag tatcggttat tccaacaggc tcgataggat tgaatgcagc cctcggcgtt
    11701 ggcggatatc cgcgtggtcg ggttatagag atatacggtc ccgaatcatc gggaaaaacc
    11761 acgttggcca ttcacgccat tgccgaagca caaaaggcag gcggtgtggc tgctttcatc
    11821 gatgcagaac atgctttcga ccggttttat gccgaaaaac tgggagtaga taccgataac
    11881 ttgcttatct ctcaacccag cagcggcgaa gaggcgttgg agattgccga gcagttgatt
    11941 cgttcatcgg cagtggatat tgttgtgatt gactctgtgg cggcgcttac ccccaagaaa
    12001 gagattgaag gcgatatggg cgattcgaac gtaggattgc aggcccggct gatgtcgcaa
    12061 gcgttgcgta aactcacttc aacgataggg aagacaaata caacctgtgt ttttatcaat
    12121 cagctaaggg agaaaatcgg agtgatgttc ggcaatcccg aaactaccac aggaggtaat
    12181 gccttgaaat tctatgcttc ggttcgtttg gatatccgcc gtattggcca gttaaaagat
    12241 ggcgatgaag tacacggaag ccagacccgc gtaaaagtgg tgaagaataa agtggcacct
    12301 cctttccgta aggccgaatt cgatataatg tacggtgagg gaatctcacg cgaaggagag
    12361 attgtggact tgggtgctga actgaacgtg attaaaaaga gcggatcgtg gtacagctac
    12421 aacgactcga agctggctca ggggcgcgat gctacaaaag cggttatcaa agataacccc
    12481 gagttggcgg acgaactcga aaagctgatc tttgaagcgt tgaaagagaa aaaataggtc
    12541 ctattaagtc tctcttcttt ctgtgttgga caaactccga cccgtacgga taattttcat
    12601 atttaaaatt aacgattatg ggtagaaaaa cgcaagggag cggctccgca ggggatgccg
    12661 tgtatggact cggagtgatt ggcgcggccg tttggtacat ctcaactgct gccactttct
    12721 ggatgggagt actgggtttt ctgaaagcaa ttgtatggcc tgctttcctc gtctacgaag
    12781 cttttaaggc tctgttgggc tgatcgctta aaactaactt ttttacccgt atgccgtcgt
    12841 gagcgtcata cgggtttttt ctatattttt gcaacaaaaa ttatagtcat gcaggaacta
    12901 aggagtttaa caccgcaaga aattgaacgg cttcaaagcc agaattgcga atgtgccgac
    12961 tggacacgtg taaaggtgag tgccgggttc aaccctcact ttattaaaaa tacccggttt
    13021 tcgggaaaga taaagttagg cgtgttggag aatgagtttg agctggcggg aggattaaaa
    13081 aaacactcct gtatcaataa cgctgttatc cacaattgtg agatcggtaa caatgtggta
    13141 atagagaaca ttcaaaatta catagccaat tacctcatcg gtgataattg cttcattcaa
    13201 aacgtagatg ttattctggt tgacgctaag acaacttttg gaaacgggac ggaagtaaat
    13261 gtgctgaacg agtcgggagg acgcgaggtt catatccacg ataaattatc cgctcatttt
    13321 gcatacgtat atgcgcttta caggcaccgc cccaacttga tcaagcgcat gaaggcgatg
    13381 gttgattatt acgccggaaa acatgcgtcg gatatgggaa gcataggaaa cgatgccatg
    13441 attgtgaacg tgggatacat caaaaacgtg aggatcggca gtttttgcaa gatcaccggt
    13501 gccatgaagc tgaaaaacgg tagcatcaac agtaacaaac aggcacctgt ctacgtcggc
    13561 aggaatgtaa ttgctgagga ttttattatt tcttcgggtt cccgaatcga tggcggatgc
    13621 attatttccc gctgctttat cggccaggca tgccacctcg atcacggata ttcagcctcc
    13681 gactcgctct ttttcagcaa ttgccaggga gagaacggcg aagcctgcgc cctttttgcc
    13741 gggccttata cggtaaccca ccataagtcc acattgctca tagccggtat gttctcgttt
    13801 atgaacgccg gatcgggatc caatcaaagc aaccacatgt acaagctggg gcccattcat
    13861 cagggaatcg ttgagcgcgg cgctaaaaca acgagtaatt cctatgtgct ctggccggca
    13921 aaggtaggcc ctttctcctt gatcttaggc cggcactacc accactcgga tacttcgaac
    13981 ttgccgttct cttatttggt ggaaaacaac aatgcgacac acctggctcc ggccataaac
    14041 ctgaaaagtg tgggcaccat tcgcgatgcc aaaaaatggc ccgaacggga caagaggaaa
    14101 gatccagaca agctcgattt tatcaatttc aacttgttga gcccctatac catccagaaa
    14161 gtatttgccg gagtaagtat tttgaagaac ttgcaggcaa cggcaggcga aacctccgaa
    14221 atctacacct atcagagttg tatcatcaaa aatgctgcct taaaaagagg actggagctg
    14281 tatgaaattg tcattcataa atttttggga aactccatca taaaaaggct cgaaaatacc
    14341 cgttttactt caaacgaaga ggtccgggat aggttgaaac cggacacaac agtcgggtcg
    14401 ggcgaatgga tagacatttc cggactgatt gccccaaaat cggaaatcga ccacttgctt
    14461 tgtcagattg aaagcggtga ggtttcgaag ttgaaggaga tcaacaaggt gtttaaacaa
    14521 cttcacgaac aatactacac cttggagtgg acttgggcat gggagaaaat tcaggaattc
    14581 tatagcgtta cccccgagac aattactgct gaagatgtta tcgctattgt ggaaaaatgg
    14641 aaaacttcgg tggtaaagct tgatgaaatg atttacaccg atgcaaagaa agagttttcg
    14701 ttgtctttca aaaccggatt cggttccgat gggaacatcc aggaccaggc catggacttt
    14761 gaatatgttc gcggagcttt tgataaaaat ctgttcgtaa tggctaccct taagcacatt
    14821 gaagataaaa aagcgttggg tgacgaactt atcgaaagga ttaaaaatgt aaaataaaac
    14881 agcctgctgt agttcatatt tgttgttgaa gaacagtttt tttattaaat gttatttttt
    14941 tagggacaaa aatgaacaaa acaacaaatg tgggtgtttt catttcaaat aagttatatc
    15001 tttgctactt cctaacgtaa aaaatgaata agaagcgaat gcatatttgt tgttgctgtt
    15061 gttgatcacc acctcccaat cgattaacaa gggctggccc tctgttcctt tcagcaaaca
    15121 aataaaaatt attcattaaa aaaactcctc ttatgataaa aaagactttt gaacttatca
    15181 ataagtttca accgttgccc gtacaaatcc ccattgacaa gtgtaagctg gaagaagtgg
    15241 ttaaaacgct cttttcgcaa atgttccctg tctgcgaacg gattgatatg ggtgacattc
    15301 atgaaaagct aaaggattgc gcccgggaat tgagtgtaaa cattgcccgc ttgaaggatc
    15361 agcagtttgc agacgaagct gtgtataaat tcttcagccg gtttcctgaa attcaggatc
    15421 ttttgtacga agatgcaaaa tgctacctca aaaatgatcc ggcagccaag tcactcgaag
    15481 aagtaatcat cacttatccc ggtttctttg cactgagtgt ctaccggatg gcgcacgaac
    15541 tgcatgtgtt gggtgtacct cttatcccgc gcctttttgg tgaatatgct cactcaaaag
    15601 tcggaatcga tatccatccc gctgctaaaa tcggcaagaa tttatttttg gatcatggca
    15661 cggggattgt gattggtgag actgccgaaa taggcgacaa cgtgaaaatc tatcagggcg
    15721 taacgctggg agcgctctac gtggaaaaaa aactgtcgga tgtaaaacgg catcccacag
    15781 tggaagacaa tgtggttatt tatgccaatg ctaccctttt gggaggagag acggttatcg
    15841 ggcacgactc aaccattggt ggcggtgcgt ggttgacaca aagcgttatc ccctattcgc
    15901 tggtttacaa ctcggtggat gttaagatca gaacggtaaa aaactttgtt cagcctcttg
    15961 actttatcat ttaaacaaaa acatataagg tattcataac attttaaaat taacaacaat
    16021 gagatttaac aacattctgg aatcaatcgg gaatacacct cacgtacgtt tgagcaagtt
    16081 gttcccccat cacgaagtat ggattaaact ggaaagacaa aatccagtag ggagtatcaa
    16141 agatcgtatt gctctggcaa tgattgaaga tgcggagaaa aaagggatct tgaagcccgg
    16201 aggaaccatt atagaaccca catcgggaaa taccggtgta gggctttctt ttgtcggtgc
    16261 ctacaaaggg tataaggtca ttatcgtgat gcccgaatcc atgtcggttg aacgacgtcg
    16321 cctgatcggg ctttttggct cggaactggt gttgacaccc cgggagaaag ggatgaaagg
    16381 tgccattgag aaagccctgg agcttcaatc cggaattccg ggatcctgga tcccacaaca
    16441 gtttcaaaac caagccaatg taacggttca tgcagagaag acggcaaaag aggtactcga
    16501 tgattttccc gaagggtttg attacctgat tacgggggta ggtaccggtg gccacattac
    16561 aggagtgggg agagagctaa aaaagaagtt tccttccatt caggtcattg ccgttgagcc
    16621 ggcggcttct cccgttattt ccggcgatag tcccggcccg cataccattc aaggcatcgg
    16681 agctggcttt atccccgaca cgttggacct gaacgtgctc gacagtgtca tcaaagtatc
    16741 caacgaagac gcctttatat acgcccgtag gctagcacga gaggagagta ttctcggagg
    16801 gatctccacc ggtgcggcta ttgctgccgt ggcgcagaaa gtgtcgcaac tgaccgagaa
    16861 aaagcggata ttgacgttca attacgatac gggagaacgg tacttgtcag tggatggatt
    16921 gtattagcgc aatcgctgcc tcaacggttt ttacattccg gatgattacc tagcgttttt
    16981 tgccggcttc c
//
LOCUS       DAOSDR010000010        16619 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrimonas sp. isolate AMR_MDS_1826
            NODE_1411_length_16619_cov_3.984484, whole genome shotgun sequence.
ACCESSION   DAOSDR010000010 DAOSDR010000000
VERSION     DAOSDR010000010.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863355
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrimonas sp. (wastewater metagenome)
  ORGANISM  Petrimonas sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Dysgonomonadaceae; Petrimonas.
REFERENCE   1  (bases 1 to 16619)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16619)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:10:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,720
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 35
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16619
                     /organism="Petrimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1411_length_16619_cov_3.984484"
                     /isolate="AMR_MDS_1826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2023866"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(24..794)
                     /locus_tag="PLI30_00890"
     CDS             complement(24..794)
                     /locus_tag="PLI30_00890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005780002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HOI78141.1"
                     /translation="MKLNLKNPLVFFDLETTGINITKDRIVEISLLKVHPNGKEEIKS
                     RLINPEMPIPAQSTAIHGITDDDVKDSPTFKQVAKSLADMLEGCDLAGFNSSRFDVPM
                     LSEEFLRAGVDFDMSKRKFVDVQIIFHKKEQRTLEAAYKFYCDKELQNAHSAEADTIA
                     TYEVLKSQLDRYPDLENDVAFLSKEYSSFNNNVDFAGRIIFDDKGVEVFNFGKHKGKP
                     VVQVLRNEPSYYSWMMDGDFPLNTKQVLTKIRLRVMIG"
     gene            complement(798..1922)
                     /gene="dnaN"
                     /locus_tag="PLI30_00895"
     CDS             complement(798..1922)
                     /gene="dnaN"
                     /locus_tag="PLI30_00895"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009000039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HOI78142.1"
                     /translation="MKFVVSSATLLSHLQAISRVINSKNSLPILDCFLLELDGNVLTI
                     TAADNETRLETKVEVNSSEGTGSLAINSKNLLDPLRELPDQPLTFDVNDETLEIYIYY
                     HNGKYNFVGLKGDEYPRPKELKESAIGLNIPAETLFNGINRTVFATADDELRPVMNGI
                     YFDITTDDLTFVASDGHKLVRVIHKDVKGDGRSSFILPKKPANLLRSLLPKENGTVDV
                     KFDENNAYITMSNFVMICRFVEGRYPNYNSVIPQNNPNNVTLDRLALLNALKRVSVFS
                     NPASSLVKLQLSEDKIVVSAQDIDFLTSAEEMIPCQYNGNVMNIGFKAVFLIDILNNI
                     PSTDVKLELSDPSRAGIILPAENEENEDMLTLLMPMMLND"
     gene            complement(2043..2939)
                     /gene="xerD"
                     /locus_tag="PLI30_00900"
     CDS             complement(2043..2939)
                     /gene="xerD"
                     /locus_tag="PLI30_00900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006798969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HOI78143.1"
                     /translation="MQRNSPVEKYKRYLLLERGLSSNTIDAYMTDLQKLIDFAETRNL
                     SFTEMKLQHLEEFLAELHDNGIKARSVARIISGIKSFFHYLVLDGFIEENPGLLLEAP
                     KIGLKLPVVLSVEEIDCLLDRIDVSTAEGARNYAIIETLYSCGLRVSELTNLRFSDLF
                     FDEGFIRVQGKGSKQRLVPISETAIQKIKNYLLHRNQQRVKKGSEDVVFLSSRGTAIS
                     RVTVFYYIRQYAEAAGISKEISPHVFRHSFATHLLERGANIRVIQEMLGHEKITTTEI
                     YTHMDRSFLRQEIIEHHPRNKK"
     gene            complement(2943..5444)
                     /locus_tag="PLI30_00905"
     CDS             complement(2943..5444)
                     /locus_tag="PLI30_00905"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOI78144.1"
                     /translation="MESSGSRSSIKYEVIIGYVLVVAIMIVGLLAVYQNLVRFSETRV
                     RNEDMSELIIVGNVINQLYEIESTQNLYTPESAADYFARYHSLRPEINAKINSLKQLS
                     KDTLRVIKLDSIEVLLDEKEENLRAIAVLLDSINKTPGIIRETVSTFVPRNLNDQIKQ
                     YIERKNVVRKSVEESDTTVVKSEQKGLFKRIGDAIRGKQDSTLIIQNRPSAADISRDN
                     IRLAIDTVVNMVRYSERLNLDSQRKFQAALLLRQSSMTNTNHLLTARIDDLLKSIEQE
                     EINKSIRLIEEKEATLNRSSKTVLGVTSLAILIALVFGLLFLMDINRSQKYRRRLEES
                     NVRINQLLRSREKLMLSISHDIKAPMSSILGYVELMESGADENTRKTYLKNMKKSGNH
                     VLQLVTNLLDYQKIESGTWLRKEMNFNLHELVENTVNSFKPLAEKKQLEYNVVNKVPG
                     DLLVFGDPFMIREIYSNLISNAIKYTLQGTVEVFVEPVNDIGKTILKFSVRDTGLGIS
                     EEYQEYIFQEFTQIKPDKIGQQEEGSGLGLAITKGLVDELGGKIHLRSERGKGSEFFV
                     DLPLETSRLTETWSSGEGCPDFDCENISVLAVDDDPIQLTMLAEMLKLKKINVVTEVD
                     PDNVLNILKNKIFDLIFLDIQMPRTNGFTLVKSMLDSDIFQSKSTPVIALSAKSDLSS
                     ADFKQLGFTDFLNKPFTSAQLFTMINKYVRITLAEEMGAVENSKGVSALIDVVKDDRE
                     SSLEILRAFVLDTGKNNNDLKTCFEKNDMENAAQVAHKILPLFKMMGDEELSDVLLKL
                     DHEKNVREDEKSKIIEAICRYIDEAKNRISEMDKR"
     gene            complement(5462..6748)
                     /locus_tag="PLI30_00910"
     CDS             complement(5462..6748)
                     /locus_tag="PLI30_00910"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOI78145.1"
                     /translation="MKRILILDDNLTICLMLKSWLVKQHYQADTATSVQEAQQKVKNE
                     AYDLILSDIRMPEADGFSFLSWIKKFDSDILVIMMTGYADIETAVESMKLGAVDYIAK
                     PIEAEVLYKKIDDALKYQENQKITEQFRDPLIRPGGEIYTRVFDKLSEVVHDDSHLLV
                     IGDKGTGKTSVAHYIYSRSRIDSGPFVTFDLDSQVLNRNGERRDYEELFLQALDKAKG
                     GLLLVKNLQKTGINFQTLLLKALSSQKKDDNFVQIIITTGEKKEQLTSVLLPKLVDSL
                     LRSYIELPTLRGNEEAILFYAEHFLGVANRQLDRKVKNIAPEVLDVFFKHSWEENIKE
                     LKNLIFKACLLTEGNTVPETILPVLFKNLAGEGTESVQPQKQVIEGLKKENYEKEKII
                     EALGIAKGNKTVAASILNIDRKTLYNKIKLYNVELN"
     gene            complement(6764..7117)
                     /locus_tag="PLI30_00915"
     CDS             complement(6764..7117)
                     /locus_tag="PLI30_00915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019540459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI78146.1"
                     /translation="MHKSKETIYQISGIVILISAVLYLFAPLVAPWIMAVSVLIFSGV
                     TALSPYPGKSVRGKRLFNFQVIACVLMIVGTYLMFRKNNLWALSMMIGAVFLLYSGVM
                     ISRELHKERSMGGNE"
     gene            complement(7258..7539)
                     /locus_tag="PLI30_00920"
     CDS             complement(7258..7539)
                     /locus_tag="PLI30_00920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006798970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HOI78147.1"
                     /translation="MKKRVLSLSVYQLIILVALIAMLFFFSDSSLLKRIKYENEIRDL
                     KTQIEYYRKQTETDKAKLNELQSSLENLEKYARENYLMKKENEEIFVIE"
     gene            7734..9386
                     /locus_tag="PLI30_00925"
     CDS             7734..9386
                     /locus_tag="PLI30_00925"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009291916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016805 - dipeptidase activity [Evidence
                     IEA];
                     GO_function: GO:0070004 - cysteine-type exopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C69 family dipeptidase"
                     /protein_id="HOI78148.1"
                     /translation="MKRFFSITVVFFFAVYATYPCTNLLVGKKASVDGSTLISYSADS
                     YNLYGELYHWEAAQYNPGTMLKVYEWDTGKYLGEIPQALKTYNVIGNMNEHQVAIGET
                     TFGGRPELSDSTGIMDYGSLIYITLQRAKTAREAIKVMTSLVAEHGYYSSGESFSIAD
                     PNEVWVMEMISKGVGNKGAVWVAVRIPDDCVSAHANQARIQQFPLNDPENCLYSPDVI
                     SFARQQGYFSGKDADFSFAQAYAPLDFGALRFCEARVWSFFNRVNKDMGKYVTYAQGK
                     TIDPMPLYIKPDKKLSVHDIQGMMRDHYEGTELDWRFDVGAGPFNSPYRWSPLTFQVD
                     SVEYCNERPIATQQTGFSFVAQMRSWLPETIGGILWFGVDDAAQTVYYPVYCGHTHVP
                     EEMAVGNGDLLTYSETSAFWTHNWVSNMVYTRYSDMNIDMQKVQQKLENNFRETQPEI
                     EKKALNLYRKSPPEAVRFLTNHTNSLIRDGLQEWKKLGQYLMVKYVDGVVKKEENGKF
                     KRNPHGQPASPERPGYSNEFYKKVIDQTGEKYKVQKVEPTVD"
     gene            9406..11157
                     /locus_tag="PLI30_00930"
     CDS             9406..11157
                     /locus_tag="PLI30_00930"
                     /EC_number="5.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008780043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016868 - intramolecular transferase
                     activity, phosphotransferases [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HOI78149.1"
                     /translation="MDNETLLAQVTDKAQLWLSGNYDEETKKEVRQMLQNEDKRQLID
                     AFYRDLEFGTGGLRGIMGAGSNRMNIYTVGAATQGLSNYLLQEFAELEQIKVVIGHDC
                     RNNSRKFAEISADIFTANGIKVFLFEDLRPTPEVSYAIRKLGCQSGIMLTASHNPKEY
                     NGYKAYWEDGAQVLGPHDKNIIAEVNRISSIEEIKFNGNKELIEIIGKDMDKAFIDKV
                     KSFVLSPQAIENQKELKIVYTPIHGTGIKLIPDALKAIGFTNVINVPEQDVISGDFPT
                     VISPNPEEPAALDLAIKRGIETEADVVLATDPDADRIGAAIRDDEGNFRLVNGNQTML
                     ILIYYLMTRRKEYGLLKGKEFIVKTIVTSELVKKIADKMGIEMFDCYTGFKWIADVIR
                     KNEGVKKYIGGGEESYGFLADDFVRDKDSVSACTLFAEVAAWAKDNGKTLYQLLQDIY
                     VEYGYSKEAGISLVRKGKEGADEIEAMMKNFRANPMKELAGSPVILSKDFVKLESVDY
                     VKEEVTTLEMPTTSNVLQYFTEDGTKVSIRPSGTEPKIKFYIEVQSSPISSREEILQA
                     EAQADEKINAVKEFFGI"
     gene            11177..11623
                     /locus_tag="PLI30_00935"
     CDS             11177..11623
                     /locus_tag="PLI30_00935"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007654467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008929 - methylglyoxal synthase activity
                     [Evidence IEA];
                     GO_process: GO:0019242 - methylglyoxal biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HOI78150.1"
                     /translation="MRKKLTIALVAHDHRKADMVDWVIFNSDFLSHHHLVCTGTTGGL
                     VRDALNEEGVFPEITCMNSGPMGGDAEIAAMVVRKEIDLAVFLIDDLNAQPHEADIMM
                     LLRQCRIHNVPIACNRYSADLMITSSLWDDLSYVPMPPRYEKFERN"
     gene            complement(11783..13909)
                     /locus_tag="PLI30_00940"
     CDS             complement(11783..13909)
                     /locus_tag="PLI30_00940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006842828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S46 family peptidase"
                     /protein_id="HOI78151.1"
                     /translation="MRKILLITAILLVALATKADEGMWLLKELNRESVERMKELGFTF
                     PVNKLYDEKNASLKDAVVIFGGGCSGVAVSDKGLIFTNHHCGYGAIQKLSSVEADYLK
                     NGFKADNLQQELYADGLTISFLRSMEEVTQQILPKVPSVLSEVQRELAIDSISDELLK
                     QYDDDPFTSVRIIPFYSGNKYYKVVYDVFRDIRLVVTPPSSIGKYGGDTDNWMWPRHT
                     GDFSVFRVYADRNNKPAKHNADNVPYKPKYVVPVSLGGVRDGDYAMTIGYPGSTQRYL
                     SSFGIAQQMESENKPRVEVRGAKQDIWWDAMTKNDTIRLKYANKYAGSSNYWKNSMGM
                     NEALVKLDVLAEKRLLESRLTSWINNSERNKAKYGHLLKTLEEAYAGSTDMARYTTYF
                     LETFNNGIELIRFANTILQFDMDGTEEDKAEFINDRIIESYKNYEPTLDRKVLPVLMR
                     LYEQRVPEKYLPDIYKKIKTEFDGDYDKYADWLFANSQFTNLTDLMNLLKDANTEMLT
                     KDPAMELALSTKDMGYELSGQMMSAYENMMRGERELMAALMEMDSRKNFYPDATFTQR
                     MSYGSVKGYKPRDGVWYDYYTTEKGVLEKYKENDPEFHLQPYIVDALQKRDFGRYAAK
                     DGTMRVNFLSDNDITGGNSGSPVFNGKAELIGLAFDGNWEALSGDIVFEPEMQRTISV
                     DVRYVLYTIDKIMNAPHIVSELKIVK"
     gene            complement(13929..15425)
                     /locus_tag="PLI30_00945"
     CDS             complement(13929..15425)
                     /locus_tag="PLI30_00945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009860557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HOI78152.1"
                     /translation="MAGGMKTLAKETAIYGVSSILGRFLNWLLTPFWTYVLMNTAQMG
                     VVGNLYSWTALTVVILTYGMETGLFRFANKSDEDPLKVYTTTLISIAFTTFLFILLAI
                     LFLNPITTIFGSEAIKPHYVLLLIIILGLDTLSAIPFAFLRYQKRPIRFATIQVLNVL
                     ITIAFNLFFFLACPWLAVKFPGAFFWFDIGKGVDYILISNLMASFIRLLLLSPQLRVK
                     YTFDGVLLKKMLVYSLPLLVLGVAGIMNQNLDKMLYPHLSTEVDKMGQLGVYTASFKV
                     AVIMVMFTQAFRYAFEPFIFAKNKESGDNKLAYSQATKYFIIFGLFIFLAVMGYLDIV
                     KYLLEVNYQEGLAIVPIVMAAELFFGIYFNLSLWYKLTDQTRWGAYLSLIGLVVTVLG
                     NILFVPEYGYMACAWTAFFANLVMMVVSYFLGQKNYPIQYDLKSAGFYVVRASFLFAG
                     IMFTHGNIGHAGLRLLVNTVLIGIYLLIVLKKDLPLQQIPIINKYFRK"
     gene            15578..>16619
                     /locus_tag="PLI30_00950"
     CDS             15578..>16619
                     /locus_tag="PLI30_00950"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007479067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase"
                     /protein_id="HOI78153.1"
                     /translation="MAQEDVFKKIVSHCKEYGFVFPSSEIYDGLGAVYDYGQMGVELK
                     NNIKKYWWDSMTLLHENVVGIDSAIFMHPTIWKASGHVDAFNDPLIDNKDSKKRYRAD
                     VLIEEYLAKIDEKIEKEVEKAAKRFGDSFDEKMYRETNPRVLENQQKRDEVHARFAKA
                     LNDSNLDDLRQLIVDCEIACPVSGSRNWTEVRQFNLMFATEMGSTAEGAMKVYLRPET
                     AQGIFVNFLNVQKTGRMKVPFGIAQIGKAFRNEIVARQFIFRMREFEQMEMQFFVAPG
                     TELDWFEYWKKERLKWHQALGFGNENYQFHDHDKLAHYANAATDIEFRMPFGFKEVEG
                     IHSRTDFDLSQHE"
BASE COUNT         4751 a         3715 c         3370 g         4783 t
ORIGIN      
        1 ggaaataaag catagcagcc ggtttaaccg atcattaccc gaagccttat tttcgtaagc
       61 acctgcttgg tgtttaatgg aaagtcgcca tccatcatcc acgagtaata actgggctcg
      121 ttgcgtaaga cctgtaccac cggcttaccc ttgtgtttac cgaaattaaa tacctcaact
      181 cctttatcat cgaaaataat ccgtccggca aaatccacgt tattgttaaa gctggaatac
      241 tctttggata agaatgcaac atcgttttcc aagtcgggat aacggtcgag ctgtgatttc
      301 aatacctcgt aagtagcaat ggtgtctgcc tctgcggagt gtgcgttttg caactctttg
      361 tcgcaataaa atttataagc tgcctccagc gttcgttgtt cctttttgtg gaagataatc
      421 tgaacatcca caaacttccg tttgctcatg tcgaaatcca ctcctgcccg aagaaattct
      481 tcagaaagca tcgggacgtc gaagcggctg gaattgaagc ccgctaaatc gcaaccttcc
      541 aacatatcgg ccaacgattt tgccacttgt ttaaaggtcg gactatcttt cacatcatca
      601 tcggtgatac cgtgaatggc tgtcgactgg gcaggaatag gcatctcggg gttgataagc
      661 cgggatttta tttcttcttt cccgttcgga tggactttca gtaacgatat ttctacaatc
      721 ctgtcttttg taatatttat ccccgtggtt tccaagtcaa agaaaaccag cgggtttttg
      781 aggtttagtt tcatttatca atcatttaac atcatcggca tcagcaatgt gagcatgtct
      841 tcgttttctt cgttttcggc aggaagaata attccggcac gcgaaggatc agaaagttcg
      901 agcttcacat cggtggatgg aatattgttc agtatatcaa tcaggaaaac agctttaaaa
      961 ccaatattca ttacgtttcc gttatactgg caaggaatca tttcttctgc tgaagtcaga
     1021 aaatcaatat cctgcgccga aacaacaatt ttgtcttccg acagttgcag ctttaccaga
     1081 ctgcttgccg gatttgaaaa tacgctcaca cgttttaaag cattcagcaa cgcaagcctg
     1141 tcgagcgtaa cgttgttggg attattttgt ggaattaccg aattgtaatt gggatagcgt
     1201 ccttccacga aacgacagat catcacaaag ttcgacatcg ttatgtacgc attgttttcg
     1261 tcaaatttca catccaccgt tccgttttcc ttcggaagga gcgacctcag caagttagcc
     1321 ggttttttcg gcagaataaa cgacgatctt ccatcccctt tcacgtcttt atgaattaca
     1381 cgcacaagct tgtgtccgtc tgaagcaacg aaagtcaggt catctgtagt gatatcgaaa
     1441 tagataccgt tcatcaccgg gcgcaattca tcgtcagccg tagcgaaaac agttctgttg
     1501 atgccgttga aaagtgtttc ggcaggaata ttgagtccta ttgccgattc tttcaactct
     1561 ttgggtcttg gatattcatc gccttttaaa cctacaaaat tgtacttccc gttgtggtag
     1621 tatatgtaaa tttcaagcgt ttcgtcgttt acatcgaaag tgagcggctg gtcgggaagc
     1681 tctctcagcg gatccaacag gttttttgaa ttaatggcca aactgcctgt tccttccgag
     1741 ctgttcactt cgactttggt ttccaatcgg gtttcgttgt cggcggctgt aatggtcaac
     1801 acatttccgt ccagttccaa gagaaaacaa tccaaaatag gtaacgaatt ttttgaattg
     1861 ataactctgc tgatcgcttg cagatggctc aacaatgtgg cgcttgatac tacaaatttc
     1921 atagggagtg aatttattta atgtttgatg ttttgatata atttagcaaa tatacaaaat
     1981 tatccgtttt gaaaagattt gtctgtccaa tttttccaag atttgtcgat aagagcattt
     2041 ggtcatttct tattccgggg atgatgttca ataatctctt gcctcagaaa acttcggtcc
     2101 atgtgagtat atatttcggt agttgtgatt ttttcatgcc ccagcatttc ctgaattaca
     2161 cggatgttcg ctcctctttc caacaggtgt gtagcaaatg aatgacgaaa aacatgtgga
     2221 ctgatctcct tgctgattcc agctgcctca gcgtattgcc tgatataata aaaaaccgtg
     2281 acacgcgaga tagctgttcc tcttgaactt aaaaaaacaa catcttcact tccttttttc
     2341 accctttgtt gattgcggtg caagagataa ttttttattt tttggatagc tgtttccgat
     2401 atgggaacca gccgttgctt gctacccttc ccctgtacgc gtataaaccc ttcatcgaag
     2461 aacaaatcgg agaacctgag gttagtcagt tcagacacac gcagtccaca actgtatagt
     2521 gtttcgataa tcgcatagtt tcgggcacct tcggcagtgg agacatctat tctgtctaac
     2581 aagcaatcga tttcctcaac agacagcact accggcagct tcaacccaat tttgggagct
     2641 tccaacaaca ggccggggtt ttcctcgata aatccgtcaa ggacaagata atgaaaaaaa
     2701 gacttaatcc ccgatattat ccgtgccacc gaacgagctt ttataccgtt gtcgtgcaat
     2761 tcagcaagaa actcctccaa gtgttgaagc ttcatttcag tgaaggaaag gttccgggtc
     2821 tctgcaaaat caatcagttt ttgcagatcg gtcatataag cgtcaatggt gtttgacgat
     2881 agccctcttt ccaagagcag gtagcgtttg tatttttcga caggtgagtt tcgttgcatc
     2941 ggttagcgtt tgtccatttc cgatatccga ttttttgctt cgtcaatata ccggcatatt
     3001 gcttcgatta tcttactctt ctcatcttcc cgaacgtttt tttcatggtc taatttcagc
     3061 aaaacatccg ataattcctc gtctcccatc atcttgaaaa gcggcaatat tttgtgcgca
     3121 acctgtgcgg cgttttccat gtcgtttttc tcaaaacagg tcttgaggtc attgttgttc
     3181 ttcccggtat cgagcacgaa cgccctcagt atttccaacg aactttcccg gtcgtcttta
     3241 acaacgtcaa tcaatgcaga aactccttta ctgttttcaa ccgctcccat ctcctcagcc
     3301 aatgtgatcc tcacgtattt attaatcatc gtgaaaagtt gtgcggaagt gaaaggcttg
     3361 tttagaaaat cagtaaaacc caactgtttg aaatcagcag aagacaaatc cgatttcgcc
     3421 gacaaggcaa taaccggcgt tgatttcgat tgaaagatgt cagagtctaa catgcttttt
     3481 acaagtgtga aaccatttgt ccttggcatt tgaatatcca gaaatattaa atcgaaaatc
     3541 ttgtttttta aaatattcaa cacattatca ggatcgactt cggtaacaac atttatcttt
     3601 ttcaatttga gcatttcagc cagcattgtc agctgaatcg gatcatcatc tacagctagc
     3661 accgaaatat tttcacaatc aaaatcggga caaccttcac ctgaactcca tgtttctgtc
     3721 aaccgcgatg tttcaagcgg aagatcaaca aaaaactcgg aacctttgcc tctctcggat
     3781 cgcaggtgaa ttttcccacc caactcatct accaaccctt ttgttatagc cagtccgaga
     3841 ccgcttcctt cttcctgctg ccctattttg tcgggtttta tctgggtaaa ctcctgaaaa
     3901 atatactcct gatattcttc actgattcct aatccggtgt ctcttaccga gaatttcaaa
     3961 atcgttttgc ctatatcatt taccggctca acaaaaacct caaccgtccc ttgtaacgtg
     4021 tactttatcg cgtttgaaat caagttggaa taaatctccc ggatcataaa cggatcccca
     4081 aaaacaagta aatctccggg gactttattg accacattgt attccagttg ttttttttca
     4141 gcaaggggtt taaagctgtt cactgtattt tcgaccagtt catgcaaatt aaaattcatc
     4201 tccttgcgca accacgtacc tgattctatt ttctgataat cgagaaggtt tgtgaccagt
     4261 tgcaacacat gatttcccga ttttttcatg tttttcaagt atgttttccg ggtgttttca
     4321 tcggctcccg actccatcaa ttccacatat cccagaattg aactcatcgg cgcctttata
     4381 tcgtgtgaaa tggataacat cagcttttcg cgtgaccgca gcaattggtt gatgcggacg
     4441 ttggattctt ccaatcttct tcgatacttc tggcttcggt tgatatccat caaaaacaac
     4501 aatccaaaaa caagcgctat caatatggcc agagaagtga cacccaacac cgtttttgaa
     4561 gaccggttga gtgtggcctc tttttcttca ataagccgga ttgacttgtt gatttcctcc
     4621 tgttcgatag attttaataa atcatcgatt cgtgcggtca gcaaatgatt agtgttagtc
     4681 attgaggatt gtcttagaag taacgcggcc tgaaattttc gctgactatc cagattcaac
     4741 ctttcggaat acctcaccat attgaccacc gtgtcgattg ccagcctgat gttatcgcgg
     4801 gaaatatcag cagcagaagg acggttttga atgatcaggg ttgagtcctg cttacccctt
     4861 atggcatccc cgattctttt aaaaagtcct ttttgctcac ttttcacaac cgtggtatcg
     4921 ctctcttcga ccgatttgcg gacaacgttt ttccgctcaa tgtattgctt aatttgatca
     4981 ttaaggtttc tgggaacaaa tgtactgact gtctcacgga ttatccccgg agttttgttg
     5041 atggagtcca acaagacagc tatggcacga aggttttctt ctttttcatc cagcaacact
     5101 tcaatactgt cgagtttaat tacccgcaac gtatcttttg aaagttgttt caacgagtta
     5161 atcttagcat taatttctgg ccttagtgaa tggtatcggg caaagtaatc tgccgcactt
     5221 tcgggagtgt acaggttttg tgtgctttca atttcgtata attgattgat aacattacct
     5281 acaataatca gttcagacat atcttcattc cggacccttg tctctgaaaa tctgacaaga
     5341 ttctggtaaa cggccaacaa ccccacaatc atgattgcta cgaccaggac atacccgata
     5401 attacctcat attttatgga agaacgtgat ccggatgatt ccattcaatt gattcaggtg
     5461 attaatttaa ttctacgttg taaagtttta tcttgttgta caaggttttt ctgtcaatat
     5521 taagtattga tgccgccacg gttttatttc ctttggcaat tccaagtgct tcgattattt
     5581 tttccttttc gtaattttct ttcttcaatc cttcaatgac ttgcttctgc ggctgaacag
     5641 actccgttcc ttcccctgca aggtttttaa aaagaacagg cagaatcgtt tcgggaactg
     5701 tatttccttc tgtcaacagg caagctttaa aaattaagtt tttcagctct ttaatgttct
     5761 cctcccaaga gtgcttaaaa aacacgtcaa gaacttcggg ggcaatattt ttcaccttcc
     5821 tgtccaactg tctgtttgcc acacccaaaa aatgttcagc gtaaaacaaa atggcctcct
     5881 cgtttccccg caaagtgggg agttcaatgt atgacctcag gagtgagtcg actagtttcg
     5941 ggagcaatac cgaagttagc tgttcctttt tttctcctgt cgttatgata atttggacaa
     6001 aattatcatc tttcttctga gaggagaggg ctttcaacaa caatgtctga aaattgatac
     6061 cggtcttctg aagattttta acaagcagta agcccccttt tgctttatca agcgcctgca
     6121 aaaacaactc ttcataatct ctccgctcac cgttcctgtt cagtacttgc gaatccagat
     6181 cgaaagttac aaacggaccc gaatctattc tgctcctgct gtaaatatag tgcgcaacgg
     6241 aagtttttcc ggttccctta tccccgataa ccaacaggtg ggaatcgtcg tgtacaactt
     6301 cactcagctt atcgaaaacc ctcgtgtaaa tctcccctcc cggccgaatc aatggatccc
     6361 gaaactgttc cgtaattttt tgattctcct ggtattttag ggcatcgtca atctttttgt
     6421 acaagacttc cgcttcaatg ggctttgcga tataatccac cgctcccagt ttcatagact
     6481 ctactgccgt ttcaatatca gcatagccgg tcatcataat taccaaaata tctgagtcga
     6541 actttttaat ccatgacagg aaagaaaagc catcagcttc gggcatcctg atatctgaaa
     6601 ggatcaaatc gtaagcttca ttttttactt tttgctgggc ctcctgaaca cttgtggccg
     6661 tatctgcctg gtagtgctgc ttcaccaacc aggatttcag catcaggcaa atagtcaaat
     6721 tatcgtctag gattaggatt cttttcatgt tataagctga aggttattca tttcctccca
     6781 ttgatctctc tttatgaagt tccctggaaa tcatgactcc cgaataaagc aagaaaacgg
     6841 ccccgatcat catcgacaac gcccacaaat tgttcttcct aaacatcagg taggtgccaa
     6901 caatcatcaa cacacacgca atcacctgaa aattgaataa tcgctttccg cgaacactct
     6961 tacccggata aggtgaaaga gccgtaaccc cactgaaaat cagcactgaa actgccataa
     7021 tccacggggc aaccaatggg gcaaaaagat acaatacagc agatattaag atgacaatgc
     7081 cggaaatctg ataaatggtt tcttttgatt tatgcattaa ttttcagttt aaacttcaga
     7141 acaatgattt ctaatgtatt taacataaaa tgtccaattc aacggtacca caaatttaat
     7201 aaaataaact tatcaggtac ttccttttca gaaaactttc gccacctcca gggtttacta
     7261 ttcaatcacg aagatctctt cgttttcttt cttcatcaaa tagttttccc gggcatattt
     7321 ttcgagattc tccagacttg actgaagttc atttaacttg gccttatctg tttctgtttg
     7381 tttgcggtaa tattcgattt gtgttttcag gtcacggatt tcgttttcgt atttgattcg
     7441 cttcagcagg ctactatcac tgaaaaagaa caacatagct ataagagcta ccaaaattat
     7501 caattgatag accgataagc ttaataccct tttcttcata atttttctga aatccataaa
     7561 gttcagctaa tcttgagaaa ctgtttgaat tttatactac aaaattaaac gaaatttcat
     7621 actaattcaa acgatttcgt ggaattaagt gaaatataac agagacagcg cgggtttttc
     7681 ccggtttttg aagtatcttt acgctttgta aactttaaaa ttcactataa aatatgaaaa
     7741 gattcttctc catcaccgtt gtttttttct ttgcagtata tgcaacttat ccctgcacaa
     7801 atctactcgt aggcaaaaaa gcatcggttg acggttcaac ccttatttcc tattctgccg
     7861 attcttacaa tctttacggt gaactttatc attgggaagc tgcacaatac aatcccggta
     7921 cgatgctgaa agtatatgaa tgggatacgg gaaaatacct gggtgaaata ccgcaggcac
     7981 tcaagaccta caacgttatc ggcaacatga acgaacatca ggtagccatc ggggaaacca
     8041 cctttggcgg acgtcccgag ttaagcgact ccaccggaat catggattac ggaagcttga
     8101 tttatattac cctgcaacgg gcaaaaactg cacgtgaagc cataaaagtc atgaccagcc
     8161 tggtagctga acacgggtat tacagttccg gcgaatcttt ctccattgct gatccaaacg
     8221 aagtttgggt aatggaaatg attagcaaag gcgttggcaa caaaggggct gtatgggtgg
     8281 ctgtccgcat tcccgatgat tgtgtatcgg cacatgccaa ccaggcacgt atccagcagt
     8341 ttcctttaaa cgatcccgaa aactgcctct actctcccga tgttatttct tttgccagac
     8401 aacaaggata tttctcgggc aaagatgccg attttagttt tgctcaggct tatgcacccc
     8461 tcgattttgg agcgctacgt ttttgtgagg cgcgtgtatg gtcgtttttt aatcgcgtaa
     8521 ataaagatat gggaaaatat gtaacgtatg cacagggtaa aactatcgat ccgatgccgt
     8581 tatatatcaa gccggataaa aaactcagtg ttcacgatat ccagggtatg atgcgcgacc
     8641 attatgaagg aaccgaactc gactggcgtt ttgatgtcgg tgccggcccg ttcaactctc
     8701 cctatcgctg gtctccactc accttccaag tagattcagt tgagtactgc aacgagcgtc
     8761 caattgctac tcaacagacc ggattctctt ttgtcgctca aatgcgttcc tggctgcctg
     8821 aaacgattgg aggtattctg tggttcggtg ttgacgacgc tgctcaaaca gtttattatc
     8881 cggtttactg cggacatacc catgtaccgg aagagatggc ggttggcaac ggcgatttac
     8941 tcacctactc cgaaacatcg gctttctgga cgcacaattg ggtgtcgaac atggtttaca
     9001 cacgatacag cgatatgaac atagacatgc aaaaagtgca gcagaaactg gaaaacaatt
     9061 tcagggaaac acagcccgaa attgaaaaga aagcattaaa tttgtacagg aaatccccgc
     9121 cagaagctgt tcgttttctc accaaccaca ccaattcttt gattagagat ggtctacagg
     9181 agtggaagaa gctcggacaa tacttaatgg tgaaatatgt ggatggtgtg gtaaagaaag
     9241 aagaaaacgg gaaattcaaa agaaacccgc acggacaacc cgcgtcaccg gaacgtcctg
     9301 gttattcaaa tgaattttat aaaaaggtta tcgaccaaac cggagaaaaa tataaggttc
     9361 aaaaagtaga accaacggtg gattaattat atattttgtt attctatgga caacgaaaca
     9421 ttattagctc aagtaaccga taaggctcaa ttatggctta gcggcaacta cgacgaagaa
     9481 acaaaaaaag aagtaaggca gatgctgcaa aacgaagaca agcgtcaact gattgatgcg
     9541 ttctacagag atctagagtt tggcacagga ggtcttcgtg gcattatggg ggcaggcagc
     9601 aaccgaatga atatttacac cgttggtgcg gctacgcaag gattatccaa ctacttgtta
     9661 caggagtttg ccgagttgga acagatcaaa gtagtcatcg ggcacgattg ccggaacaac
     9721 agtcgtaaat ttgccgagat atctgccgat attttcaccg caaacggaat taaagtgttt
     9781 cttttcgaag acttacgtcc gacaccggaa gtttcatacg ccattagaaa actcggttgt
     9841 cagagtggaa ttatgcttac agcatcacac aatcccaagg aatacaacgg atataaggcc
     9901 tattgggagg atggagcaca agtacttggg ccgcacgaca aaaacattat tgctgaagta
     9961 aacagaatct catcgataga agaaattaag ttcaacggca acaaggaatt gatcgagatt
    10021 atcgggaaag acatggacaa ggcctttatc gacaaggtta aaagttttgt tctttctccc
    10081 caagctattg aaaatcaaaa agaattaaag attgtttata cacccatcca tggaaccggc
    10141 atcaagctta tccccgatgc gctgaaagcc attggattca cgaatgttat caacgtaccc
    10201 gagcaggacg taataagtgg agattttccg acagtcatct ctcccaatcc cgaggagccg
    10261 gcagcattgg acttagctat aaaaagaggt atcgaaacag aagctgatgt tgttttagcc
    10321 actgatcccg atgccgacag aatcggagca gccatccgtg acgacgaagg caattttagg
    10381 ctggtcaatg gtaaccagac catgcttatc cttatttatt acctgatgac acgccgcaaa
    10441 gagtatgggc tgttaaaggg taaggaattt atcgtaaaaa cgattgtcac ctccgagttg
    10501 gtgaaaaaaa tcgcagacaa gatgggcatc gaaatgttcg actgttacac cggattcaaa
    10561 tggattgccg acgttatccg caagaacgaa ggggtaaaaa aatacatcgg tggaggagaa
    10621 gaaagctacg gattcctcgc cgacgatttt gtgcgcgaca aagattcggt atcagcctgc
    10681 acgctgtttg cagaagttgc agcctgggca aaagataatg gaaagacgct gtaccaactg
    10741 cttcaggata tctatgtaga atatggatat tccaaagagg caggtatttc attggtgcgc
    10801 aaaggaaaag agggtgccga cgaaattgaa gccatgatga aaaacttcag ggcgaacccc
    10861 atgaaagaat tagcaggctc acccgttatt ttatcgaagg atttcgtgaa actggaaagt
    10921 gtggattatg ttaaagaaga ggtgaccaca cttgagatgc ccacaacatc taacgtgtta
    10981 caatacttta cagaagatgg cacaaaagta tctattcgcc cttccggaac tgaacccaag
    11041 ataaaattct atatcgaggt gcaaagctct cccatatctt cgcgcgaaga aattctacaa
    11101 gcggaggctc aggcagatga aaaaattaat gctgtcaaag agttcttcgg aatttaacta
    11161 tcgaataact attaaaatga gaaaaaaact gaccattgcc ttggtggccc acgatcatcg
    11221 aaaagccgac atggtcgact gggttatctt taattccgat ttcctatccc accatcatct
    11281 ggtctgcacg ggcacaaccg gcggactggt tcgtgatgca ctgaacgaag aaggagtatt
    11341 tcccgaaatt acctgcatga attccggccc tatgggtgga gatgctgaga tcgcagccat
    11401 ggtggtacgc aaagaaatcg atctcgccgt gttcctgatc gacgacctga acgcacagcc
    11461 gcacgaggca gacatcatga tgctgctgcg ccagtgccgt atacataacg tacctatagc
    11521 ctgtaaccgt tacagcgccg acctgatgat taccagcagt ctgtgggacg acctgtctta
    11581 tgtccccatg ccgccgcgat acgaaaaatt tgaacgaaat taatgagacg ggaaacaaga
    11641 gactgtctca aaataaaaaa caaccctact gatacaatga atattcggat ttgttcccgg
    11701 acaggtgaat aaaaagaaga aagagggtga aatcacacat tgagattcac cctctttctt
    11761 gtatgttttt taataacaaa gattacttta caatctttag ctcactgacg atatggggtg
    11821 cgttcatgat tttatcgatt gtgtaaagca cgtaacggac atccacactg atcgtacgtt
    11881 gcatctcggg ttcaaagaca atgtctccgc tcaacgcctc ccagtttccg tcgaacgcca
    11941 atccgataag ttccgctttc ccgttaaaaa cagggcttcc cgaattacct ccggtaatgt
    12001 cgttatcgga aaggaaattt acccgcatag tcccatcctt agctgcatat cttccaaaat
    12061 ctcttttttg gagagcatca acgatgtaag gttgtaaatg aaattccgga tcgttttcct
    12121 tatatttttc caacactcct ttttcggtcg tataataatc ataccagacc ccgtcgcgcg
    12181 gcttatatcc tttcaccgat ccataactca ttctttgggt aaacgttgca tcgggataaa
    12241 aattctttcg cgagtccatc tccatcaatg cagccatcag ttctcgctca cccctcatca
    12301 tgttttcata tgccgacatc atttgccctg agagttcgta tcccatatct ttagtcgata
    12361 atgccagctc catagccggg tctttggtca gcatttccgt attcgcatct ttcaacaaat
    12421 tcatcaaatc agtcaggttg gtaaattgag aattggcaaa caaccaatcg gcatacttgt
    12481 cgtaatcccc atcaaattcg gttttgattt ttttgtaaat atcgggaagg tacttttccg
    12541 gaactcgctg ttcgtatagc cgcattaata caggaaggac tttccggtca agtgttggtt
    12601 cataattttt atacgactca atgatcctgt cgttgataaa ttcagccttg tcttcttccg
    12661 tcccatccat atcaaactga aggatggtat tggcaaaacg gatcaattcg attccgttgt
    12721 taaacgtttc gagaaaatag gtcgtgtaac gagccatatc agtagaaccg gcatacgctt
    12781 cttccagtgt ttttagcaga tgaccgtatt ttgccttgtt tctttcgcta ttgttgatcc
    12841 agcttgtcaa tcggctttcc agcaggcgct tttccgccaa tacgtcaagc ttaacaaggg
    12901 cttcgttcat acccatggag tttttccagt agtttgagct tccggcatac ttgttggcat
    12961 atttcaaccg gatagtatcg tttttagtca tggcatccca ccagatatcc tgtttggctc
    13021 cacgcacctc aacacgcggt ttgttctccg attccatttg ctgtgcaatt ccaaacgacg
    13081 aaagatagcg ttgtgtgctt cccggataac cgatggtcat ggcatagtcg ccgtctctca
    13141 ctcctcccaa ggaaaccgga accacatatt taggcttgta cggcacatta tcggcgttgt
    13201 gtttagccgg tttgttgttc ctgtccgcat acacgcggaa aaccgaaaaa tcacccgtgt
    13261 ggcggggcca catccagtta tccgtatcgc ctccgtattt tcctatcgac gatggtgggg
    13321 ttaccaccaa acggatatca cgaaaaacat cataaactac tttgtagtat ttgttccctg
    13381 aataaaaagg aataatccgt accgaggtaa agggatcgtc gtcgtattgc ttgagcagtt
    13441 catccgagat agagtcgatg gccaattcgc gttgtacttc gcttaacacg gaaggcacct
    13501 taggcaaaat ctgctgggta acttcctcca tactgcggag aaaagaaata gtcaacccat
    13561 cagcatataa ttcctgttgc aaattatcgg ctttgaagcc atttttcagg taatccgctt
    13621 caaccgaact tagtttttga atagcaccgt acccgcaatg gtggttggta aaaatcaacc
    13681 ctttgtcgga tacggcaaca ccggaacaac cgccgccgaa aatgaccact gcatctttca
    13741 gcgaagcgtt tttctcatcg tatagtttgt ttactggaaa agtgaatccc agttctttca
    13801 ttcgctccac actctcccga ttaagttctt tcaaaagcca cattccttcg tcggctttgg
    13861 ttgcaagtgc cacaagcaat atggcagtaa tcaatagtat ctttctcatt tatttcaaat
    13921 taagtatatt atttcctgaa atatttattt ataatgggta tctgctgcaa aggtaagtcc
    13981 ttttttaaaa ctataagcag gtaaattcct attaaaacag tgtttacaag taaccttaat
    14041 ccggcatggc caatatttcc gtgagtaaac attattcccg caaacaggaa cgaggctctg
    14101 acaacataaa atcctgccga ttttaaatcg tattgaatgg gatagttttt ctgaccgaga
    14161 aaataagaaa ccaccatcat gacaaggtta gcaaaaaatg ctgtccaggc acaagccatg
    14221 taaccgtatt ccgggacaaa taagatattt cccaacaccg ttacaacgag tccgattaag
    14281 gataaataag cgccccaccg ggtctgatcg gtcagtttat accacaacga cagattgaag
    14341 tagataccaa aaaacaactc tgctgccatc acgattggaa ctatagcaag cccctcctga
    14401 tagttaacct ccagcaaata ttttacgata tcgagatacc ccatgacagc caggaaaata
    14461 aacagcccaa aaataatgaa atacttggtt gcctgggaat aggccaactt attgtcacct
    14521 gattctttat ttttagcaaa gataaacggt tcgaaagcgt atcggaaagc ttgagtgaac
    14581 atgaccataa tcaccgcaac cttaaacgaa gccgtgtaaa cgcccaactg ccccatctta
    14641 tctacttcgg tagaaaggtg cggataaagc attttatcca ggttctgatt cattattccg
    14701 gcaacaccca gcacaagcaa cggcagggaa tagaccaaca ttttcttcag caaaactccg
    14761 tcgaaagtat atttcaccct caattgcggt gaaagcagca gtaaccggat aaacgacgcc
    14821 atcaggttgg atatcaaaat atagtcgact cccttgccta tatcgaacca gaaaaaagct
    14881 ccggggaatt ttacggcaag ccaaggacag gccaggaaga aaaaaagatt gaacgcgatg
    14941 gttatcaaaa cattcaggac ctgtatggtt gcaaaccgga tgggacgttt ttgatacctc
    15001 aaaaaagcga agggaattgc actgagcgta tctaatccga gtatgataat cagcagcaga
    15061 acatagtgtg gttttatagc ctcacttcca aaaatggttg ttataggatt taaaaataag
    15121 attgctagga gaatgaacaa aaacgtcgta aaagcaatac tgataagcgt tgttgtataa
    15181 accttgagtg gatcttcatc gcttttattc gcaaaccgga acaatccggt ttccatccca
    15241 tacgtaagaa ttacaacggt gagtgcagtc cagctgtaca gattgcccac cactcccatt
    15301 tgggcagtat tcatcaaaac ataagtccaa aagggggtaa gcaaccagtt tagaaatctg
    15361 cccaatatac tgcttactcc gtaaattgct gtttcctttg caagggtttt cattcctcct
    15421 gccatacatc caaatctttg aaattaacgg caaaagtaca taaaataggc ttttgtttgc
    15481 caatttgagg cataattata catcttttaa atagcgttag gatggaactt tcgaaaaaaa
    15541 ccgtttactt tgtagcttta ttgaggaatc gtgatttatg gcacaagaag acgtttttaa
    15601 aaagattgta tcgcactgta aagagtacgg atttgttttt ccatcgagcg agatttacga
    15661 tgggttaggc gccgtttacg actacgggca aatgggtgtt gaactgaaaa acaacatcaa
    15721 aaaatattgg tgggacagca tgaccttact gcacgagaac gtggtgggga ttgattctgc
    15781 catttttatg catccaacta tatggaaagc ttcggggcac gtggatgctt ttaacgatcc
    15841 gctgatagac aataaagaca gcaaaaaacg ttatcgggcc gatgtattga ttgaagaata
    15901 cctggcaaag attgacgaga aaatagagaa ggaagttgaa aaggcagcca aacggttcgg
    15961 ggacagcttt gatgaaaaga tgtatcgcga aaccaatccg cgcgtattgg agaatcaaca
    16021 aaaacgcgac gaggtccatg cccggtttgc aaaagcattg aatgacagta atcttgatga
    16081 cctgcgtcaa ctcattgttg attgtgaaat agcctgtccg gtaagcggat ccaggaactg
    16141 gacagaagta cgccagttca acctgatgtt tgccacggaa atgggctcta ctgctgaggg
    16201 tgccatgaag gtatacctcc ggccagaaac ggcgcagggt attttcgtca acttccttaa
    16261 tgtgcaaaaa accggtcgga tgaaagttcc tttcggcatc gcacagattg gcaaggcttt
    16321 tcgaaacgaa atcgttgccc gtcagtttat tttccgcatg cgcgagttcg aacagatgga
    16381 gatgcagttt tttgtagcgc cgggcacaga actcgactgg tttgaatatt ggaaaaagga
    16441 gcgattaaaa tggcatcagg ctctcggttt cggcaatgaa aattatcaat ttcacgacca
    16501 cgacaagttg gcccattacg ccaatgccgc tacagacatt gagtttcgca tgcctttcgg
    16561 ctttaaggaa gtggaaggaa ttcactcccg caccgatttt gatttgtccc aacacgaaa
//
LOCUS       DAOSDR010000011        16171 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrimonas sp. isolate AMR_MDS_1826
            NODE_1460_length_16171_cov_4.200174, whole genome shotgun sequence.
ACCESSION   DAOSDR010000011 DAOSDR010000000
VERSION     DAOSDR010000011.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863355
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrimonas sp. (wastewater metagenome)
  ORGANISM  Petrimonas sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Dysgonomonadaceae; Petrimonas.
REFERENCE   1  (bases 1 to 16171)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16171)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:10:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,720
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 35
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16171
                     /organism="Petrimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1460_length_16171_cov_4.200174"
                     /isolate="AMR_MDS_1826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2023866"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..362
                     /locus_tag="PLI30_00955"
     CDS             <1..362
                     /locus_tag="PLI30_00955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI78154.1"
                     /translation="AGALNDNWTISTNLQSRDGSFESARMGLDLSTRLLDDRLRISTN
                     LSYGDKATTASDQAFMGEFELEYDINNWLMIRVYNRANQRFSKRAPTTQGTGIVVTRD
                     ARTFRDLFRFSFRKKED"
     gene            392..2680
                     /locus_tag="PLI30_00960"
     CDS             392..2680
                     /locus_tag="PLI30_00960"
                     /inference="COORDINATES: protein motif:HMM:NF013283.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HOI78155.1"
                     /translation="MKYSFCIFLVLLLISCDATKKVPSGSYLLNKVEIDTDTKGASES
                     DLKPYLRQKPNSSMFIFGKIKLHAYNIPENDSTWLNRQFLKFGEPPVLYSDRLTGLSM
                     EQIRLQLGNKGYLNAEVDTVVDKKDRKASVTYKVTGNDPYLVRTFRDSIYSVDSTIYK
                     ILDEKKLLNVIKEGDAFDLEVLENGRIKITNELRNNGYYNFSKENFYFLADTTVGTHQ
                     VDLTLGLNNPTDSTLHSSYRFGEVIVNNGVSSTILQDSSKHYLLDTVQYRDVKVVQER
                     DKFMRPAAIFYNTFIRPTRVYSDQLVERTYSSLNRLGSVSQTAINLTPVVVNDSNYIN
                     ANISIYPGNMHFMQFGIDGTHAAGDLGIATDATYEHKNILKGGETFRIKLNGAYEFIT
                     ASDSASLIDKSYYEYGAEAFLSIPQLLLPWVMKQLRDQPSASTEFSVGVNFQNRSEYL
                     RQFFNLSSRLQWARRNWQVTNVVEPLDITYVRMPRMSEKFKNAYMNDSINPILKASYE
                     EQMIVRTAYNVSYTNVSSGQLPPRLPFRVRAGIEVAGYLPRIVAAMGGTRKNTSGREM
                     FLGIPYAEYVKTDFDFAPAYRWNDKNTLAGHIALGMAYPYGNSIVLPFEKRYYGGGAN
                     GVRGWSTRTLGPGTYNNDSLGYDFANKTGDIKLDMSLEFRRKLTKLFELAGFVDVGNV
                     WTIKDYNSQPGGYFSWNSFYKELAAAYGMGIRLDLNFLLLRVDLGMKAHNPALPEGQR
                     WTIFSPDFHRDFAFHFAIGYPF"
     gene            complement(2661..4883)
                     /locus_tag="PLI30_00965"
                     /pseudo
     CDS             complement(2661..4883)
                     /locus_tag="PLI30_00965"
                     /inference="COORDINATES: protein motif:HMM:NF019583.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GH92 family glycosyl hydrolase"
     gene            complement(4896..6086)
                     /locus_tag="PLI30_00970"
     CDS             complement(4896..6086)
                     /locus_tag="PLI30_00970"
                     /inference="COORDINATES: protein motif:HMM:NF019736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI78156.1"
                     /translation="MEKKSILIFGAGKIGRSFIGQLFGLSGYEVVFSDIDEELVSLLN
                     HEKSYAVVIKGDKEERITISNVRAINGTNQEAVTEEVKNTSIIAVSVGKNALPKIIPT
                     LAKGLIKRFQGSENGPVDIIIAENMRSANEVIYKELKDNLPDDFPLHDYVGLVETSIG
                     KMVPLMTAGEIKKDPLSVFAEPYNQLILDKKGFRNEVPDVMGLAPKENIRAWVDRKAF
                     IHNLGHATAAYYGFFKYPGSTYLYEVLEDTHVIEFTREAMLQSAAVLLKIYPDDFTPR
                     DLSVHIEDLLFRFRNRSLKDTLFRVGQDIPRKLGPDDRFMGIIRLAIPLRMPYDKILE
                     AMSYAFFFKATDENGHRSEQDIVFEKLLSQGIDYTLQKVCGIDPVFDQELAEKVKSSI
                     NVKR"
     gene            complement(6097..7287)
                     /locus_tag="PLI30_00975"
     CDS             complement(6097..7287)
                     /locus_tag="PLI30_00975"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HOI78157.1"
                     /translation="MLKKKITVSGTGCCLVDRLYNNVSFYSNAFLSYSSKKRGDGGLS
                     PGHLVLKEEFEEYTKKNFQVILNELTAGRAPDKINVGGPCIVALIHAAQITFGLNSEI
                     KFYGCHGPDENGSFLLSSLQKLPVNISHFKQMGNLTPSTDVLSDPSYDNGHGERVFVN
                     SIGSAWDYSPENLDNDFFKSDIVVFGGSALVPQIHDNLVELLQQSKENGCITLVNTVF
                     DFVNEKANPHKRWPLGKNDESYPNIDLLITDRDEALRLSGEKSIEKAMQFFIEKQTGA
                     VIITNGAKNVLLYAGNELFGRVEMRELPVSDAISKRIREKVYNGDTTGCGDNFVGGVI
                     GSLMFQLNEGKMKLDLEAATTLGIVSGGYACLYIGGTYFEKYPGEKYELMTPVLNDYK
                     KQIK"
     gene            complement(7300..7977)
                     /locus_tag="PLI30_00980"
     CDS             complement(7300..7977)
                     /locus_tag="PLI30_00980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019540936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lyxose/D-mannose family sugar isomerase"
                     /protein_id="HOI78158.1"
                     /translation="MKRSEINQILREAKEFLHEKNFLLPPWAYWTIDEWRENKKNAEE
                     IFNNMLGWDITDFGSGDFHSRGLFLFTIRNGKFNVDKKPYAEKIMIVEENQETPMHYH
                     WSKMEDIINRGGGNLVIELYNSTLDNQLDTTDVHFKKDGIKGWVEAGGKVVLTPGESI
                     SLEQGMYHRFYGEPGKGKVLVGEVSMVNDDSADNCFHEIIGRFPTIVEDEKPLYLLVN
                     DYNTFLS"
     gene            complement(8013..10403)
                     /locus_tag="PLI30_00985"
     CDS             complement(8013..10403)
                     /locus_tag="PLI30_00985"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH92 family glycosyl hydrolase"
                     /protein_id="HOI78159.1"
                     /translation="MTKSQHAIKINMNRLKILLILSVMAVPHLRSVSQERPSDLANPL
                     IDTHKSRYDYFISAALPFGMVSLSPDMKHGEMWNSGYLYDEKYILNFSHVHNGQTAGI
                     PVMPVVGECKGYLGLEASKSEFSHDKEVVKVGYHKVFLEDSQITAELTATCRVGMHRY
                     TFPAADTAHFIFDLSAALGATKTSYAYARKISSNEIEGYSLLSPTFRRKKPYLIFFVA
                     QFDKPFDSFNGWEKNKEGIRKIIRPEENLISGDTIGAYVTYRGLKNNEQICIKVGISF
                     VSTENARMNLSSELPHWNFDKVVKDAAEAWDNYLSRVLVEGGTREQRIKLYTDLMHTA
                     SKRISDDVDGSYMDWTGMYPVARKLPLGSDNKPTRHFIEGDGLWGGQWNLNVFWTLLY
                     PEYGNWMVETFLDYYRNAGTMARCSWGGNYSYVMVGDHTTPLIAALLSSKRATFDPVS
                     AYTGARKNAFPGGIRDRGGYEAGPHPSGGGMDWYVEKGYVPIEIRERGEGFHREGAAM
                     TLEYAYQDWCLAQMAGILKKSEDRKLFIQRSENWRNVFDPAIGWSRPRHVSGEWLKDF
                     TPVVEKGKFNSPGFIEGSSATYTFYVPQNMEGLIQEMGGNKKFIEKLESNFLKAKDFR
                     FIAPHGEHGSAWVDYENQPSLAMAHLFSHAGAPWKTQYWVREVKEKAFGGVDPYSGYN
                     GDEDQGMLGALGVLMAIGLFDLHGCVGEFPELEITSPLFDKIVLRFPSLADPLQNTLF
                     RISVKKKNPADIYIQHAILNGIKWSRFQFPVTDFLNGGELELELGPYPNKKWGKPF"
     gene            complement(10400..11416)
                     /locus_tag="PLI30_00990"
     CDS             complement(10400..11416)
                     /locus_tag="PLI30_00990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI78160.1"
                     /translation="MVILENNNVLLKINLDGGSYSDFHLKDQPLNPINWVIKDETQPP
                     FKGHFLCFDRWGPPSEAEKSNGFTHHGEVNSQRWELINHAATSTSNECVMCCTLPMGG
                     LEITRQVSLSGEEPLFFVSEKIRNLNKYGRMFNLVQHVTVAPPFLDRKTLFDNNTEKG
                     FEDKEDGSLHQEEPVLCWPEAVHKDGKVDLRHFQDPWPRVSSFIYNRRETYGWVTASN
                     PTLGVMLGYLWKVEDYPWINFWRSMENGNPVAFGMEFGTTGLHEPFTVVAKKGKIFDR
                     NLYEFIDAQETIEKTFLAFLARIPEDFNGVDNIRLEDSNLVIRERGRTDRNIRYKFKR
                     HYLG"
     gene            complement(11426..12802)
                     /locus_tag="PLI30_00995"
     CDS             complement(11426..12802)
                     /locus_tag="PLI30_00995"
                     /inference="COORDINATES: protein motif:HMM:TIGR00879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0051119 - sugar transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar porter family MFS transporter"
                     /protein_id="HOI78161.1"
                     /translation="MQNLNTVVSEKRINLTGISLIAALAGFIFGFDTVVISGANLPIK
                     ELWNTSPLFHGFFIMSMALWGTVIGAIFGGFPTEKFGRKKVLLSVGILFSLSTIGTAL
                     ATNPYSFSFFRFIGGLGIGISSVVAPIYISEISTPANRGKLTGMYQFNIVFGLLIAYI
                     SNYFLKGFDGANDWRWMLGVMIIPSLVYALLVIFVPESPRWLVMKNREGEAEEIITRL
                     GINHPKEEVQTIANHIREQMKRKKSSLFIPRYKRILWLAFFIAFFNQWSGINFILYYA
                     PEILERSGLAARDSLMNSILLGGINIIFTFLGLYLIDKLGRKKLLIIGSFGYIISLVM
                     VAWSFYTHAGSMVLLVFLLLFCASHAIGQGTVIWVFISEIFPTGIRAKGQSFGASIHW
                     VFAALITLITPVFLDSERGVFRENPWPIFAFFAFMMVLQLIWVLQKVPETRGISLEAL
                     SKKLIPDK"
     gene            complement(12827..15490)
                     /locus_tag="PLI30_01000"
     CDS             complement(12827..15490)
                     /locus_tag="PLI30_01000"
                     /inference="COORDINATES: protein motif:HMM:NF013256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004559 - alpha-mannosidase activity
                     [Evidence IEA];
                     GO_process: GO:0006013 - mannose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 38 C-terminal
                     domain-containing protein"
                     /protein_id="HOI78162.1"
                     /translation="MKAILITLCVSWVSLSSFAMFNEPLVPDVSKKDTIHVVGHAHMD
                     MNWLWTYSETMKMCNDNLRQAVAFMKEFPDYTMIQSQASVYNFVEKVDPPLFEEVKNY
                     VKEGRFEPVGGMWTEGDMNLSSGEAITRSFLLGQRYFQKHFGKMATIGWLPDNFGHVS
                     QMPQILKLAGCNYFYFHRCKPYIGTFWWEGTDLSKVLCYANDTYNGDINLKLKDELTK
                     FDPKGHRILQITGVGDHGGGPTRANIEMVHQLDKMADYPAVKFTTAKDFFKKSAVEAK
                     DKPTHHGEMQFIFEGCYTTVSEIKSGNRNNENMLYSNEFFNTLRWLKGDNYPAEEIRD
                     LWITQTFNQFHDILPGSAINEANKEAVARYMENLRKSTELRDNAFRKTADEIKFQTNM
                     GQPVVAFNLQPVSRKTVVEANVYSHTQPASVKSTSWGDYYGSKNITPIDKGQGNYPTV
                     LVRDASGKTYPAQIVWSKVTPPGFTSKIQFIVDEMPAGGYKTFYVDMTQPGTFNEPIP
                     FENNTFETDFFEVKVNMKTGSISSLYDKRNGKEYVQEGKELNRLKMYLEDKKGGMKSW
                     TLNKIVKEEEVDDVRSVKVVENGPVRACIETVKYWGKSSFTERTYIYRSYPRIEYEME
                     VHWLETGTDSTDSPMLRAHFPLAFDAPRFYCQVPFDVVERPANGKFQGGAVPRHLTSQ
                     SNVYGVDPERGDGQEVPAQKWVDVTDGQAGIALLNKTKYGHSYHDGELRLTLMRAAGS
                     PDIYPNLGKFVISYALYPHAGDWKNGVWVEGDDYNVPVLAAEPPSLALIKDHATQPEE
                     KSFFSVAQPNIAITGIKQSEEGNELIIRMVETEGKATTATINLPVAATSARRLNLIEM
                     PLQGVSSPALQGTTLSVQLKPHEIATIGIKP"
     gene            complement(15527..>16171)
                     /locus_tag="PLI30_01005"
     CDS             complement(15527..>16171)
                     /locus_tag="PLI30_01005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 38"
                     /protein_id="HOI78163.1"
                     /translation="GGILTQGQQLPGSSSDWNVAQNFVSVRSSKGQVVVVSNEVPLWQ
                     FSDFNMGKFERYPKQGLPWLYSWVMNNYWFTNFRAYQEGTFSWSYQLTSTNDTTNAFA
                     TKFAWGERNPFATRTLPAGENKLCNASLRTLKIDGPENVLVINIRPSFHKKSIILHLR
                     ELEGLPADIKLSPADQTLTIKKVTEINSLGERTGQPISAIRLKPYEVKFIEVEI"
BASE COUNT         4200 a         3705 c         3482 g         4784 t
ORIGIN      
        1 ttgccggagc cctgaacgat aactggacca taagcaccaa cctccaaagc cgggatggaa
       61 gttttgaaag tgctcgaatg gggttggatc tttccacacg tttacttgat gaccggcttc
      121 gaatttcaac aaatctcagt tatggagaca aagcaaccac cgcgagcgat caggcattta
      181 tgggagagtt tgagttggag tacgatatta ataactggct gatgatacgt gtctacaaca
      241 gggcgaacca acgattctcc aaacgggcgc ccactacaca aggaactggg attgtggtga
      301 cacgggatgc ccgaaccttc agggatttgt tccggtttag tttcagaaaa aaagaggatt
      361 agaacaaaga gcaaagaaca aaaataaaca gatgaaatat tctttctgca tattccttgt
      421 tttactgttg atttcgtgcg atgccacaaa aaaagtgccg agcggaagct acttgctgaa
      481 caaggtcgaa atagatacag ataccaaagg agccagtgag tcggacctga agccttacct
      541 tcggcaaaag cctaactcgt cgatgtttat tttcgggaaa ataaaattgc atgcctataa
      601 tattcctgag aatgactcca catggctcaa tcgccagttc cttaagtttg gcgaaccgcc
      661 tgtgttgtac agcgaccgcc tcaccggact ttcgatggaa cagatcaggc tgcagctggg
      721 taataaagga tatttaaatg cagaggtaga tacggtagta gataagaaag atcggaaagc
      781 cagtgtgact tataaagtta caggtaatga cccttacctg gtcagaacct tcagggactc
      841 tatctattct gtcgatagca caatatacaa aatactggat gaaaagaagc tcttgaatgt
      901 gataaaagaa ggagatgctt ttgatctgga ggtactggaa aacggacgaa taaagataac
      961 caacgaactg agaaacaacg gatattataa tttctccaag gaaaacttct atttcctggc
     1021 cgataccact gtcggcaccc atcaggtgga tttgacactg ggattgaata atcctacgga
     1081 cagtactttg cacagcagct acaggtttgg agaagtgatt gtaaataacg gagtcagttc
     1141 taccattcta caggacagtt ccaagcatta tctgttggat accgttcaat acagggatgt
     1201 gaaagtcgta caggaacggg ataagtttat gcgtccagca gctatttttt acaatacctt
     1261 tattcgtccc actagggttt attccgatca attggttgag agaacctatt cctcgctgaa
     1321 ccgtttgggc tcggtaagtc aaacggcaat caatttaaca ccggttgtag taaacgattc
     1381 taattatatt aacgctaata tttccattta tccgggaaat atgcatttta tgcagtttgg
     1441 tattgatgga acacatgctg ctggagactt gggtatagcg acagatgcaa cctacgaaca
     1501 taaaaatata ttgaagggtg gggaaacttt tcgcatcaag ctgaacggag cctacgagtt
     1561 catcacggca tccgatagcg ccagccttat tgataaaagt tattacgaat acggtgcgga
     1621 agcattttta tcgatacctc agctcctgtt gccttgggtg atgaagcaat tgagagatca
     1681 accttcggca tctacagaat tctctgtcgg agttaatttt caaaacagga gtgaatacct
     1741 gcgccagttt ttcaaccttt cgtcccgcct tcagtgggct cgtcgtaact ggcaggtaac
     1801 caatgtggta gagcctctgg atattacgta tgtgagaatg ccccggatga gcgaaaagtt
     1861 caaaaatgcg tatatgaacg atagcatcaa tcccatttta aaagccagtt atgaagaaca
     1921 gatgattgtc cgtacagcat acaacgtcag ctatacaaac gtgtcttccg gacagttacc
     1981 accccggtta ccctttcgtg tacgtgccgg aatagaagtc gcgggttatc ttccccgtat
     2041 agtggcagcg atgggtggaa cacgaaagaa cacatcgggc agggaaatgt ttttgggaat
     2101 accttatgcc gagtacgtga aaaccgattt cgattttgct cctgcttacc ggtggaacga
     2161 caaaaataca ttggccggac atattgcgct tggtatggct tatccttacg gaaattccat
     2221 tgtcctgcct tttgaaaaac gatattacgg cggcggggcg aatggtgtga gaggatggag
     2281 tacgcgaaca cttggtcccg gaacttataa caacgatagt ttaggttacg atttcgccaa
     2341 caagaccggt gatattaaat tggacatgag tctggagttt cgaagaaaat tgacgaagct
     2401 ttttgaactg gccggttttg ttgatgttgg aaacgtatgg acgataaaag attacaactc
     2461 acaacccgga ggttatttta gttggaacag tttctataaa gaacttgcag ctgcatacgg
     2521 aatgggtatc cggctcgatt tgaatttcct cttactgcgc gtcgatctgg gaatgaaagc
     2581 ccataaccca gccttgcctg aagggcaacg gtggactatc ttcagtcccg attttcaccg
     2641 tgactttgcc ttccattttg ctatcggata tccgttctga tacctggtgc atagtttaac
     2701 gatgaaggag ggctgtctgc ggagctgttt ccccacaact tgtttggcgt tttcctcatt
     2761 tccagttcca atatgctccc gttaatgata tcgtcgtgag taaaccaagg tttgtttaag
     2821 gtttttccgt ttataaacgc ctgttggata tagatgttct caccggtatt gcctttcgca
     2881 attatcctga acacttttcc gttcggcaga tttattttta tctcgttaaa cacggggctt
     2941 cctatattat aaaccgggat gccgggtgtg accggaaaaa atcccatcat cgacaaaact
     3001 acaaaagccg acattcctcc accgtcttca tctccgggta tgccgaagat attgtccaga
     3061 aagaatgtgt ccagtaacat ccttattctt ttctgggtct tccagggaac tcctaaataa
     3121 ttgtagatat agggaatgtg aaaactgggc tcgttgccca taacaaattg tcctacaagc
     3181 cccgatgcgt cgggttgagt gagccaaaac ctgaacttcg gcaatcctaa atcttcccgg
     3241 aaaagctgat ctagttttaa ttctgccgcc ttacgcccgc ccatcagttc aaataagcct
     3301 tcaagatcgt gtttcacatc ccaattgtaa gtataggcat tgttttcggt aaaatactct
     3361 cttcctgcaa aacggggatc gtaaggttca atccagtttc cccgtttgtc tttcggccac
     3421 ataaaccccc tttctgcacg aaacacattt ttatagttct ccgcccgttt taaaaaaagg
     3481 tctctttctt gcatcagctt taattccgag gccagttgcg cgatacacca gtctgaatag
     3541 ctgttggcag ttgtgacgga aacgctttgt cgtttttccc aaacggtgtc tactgtcgaa
     3601 acggtctctc tttcccccgg aggtagtccg ggcatataac cgttttcgtt gtaaaaatcg
     3661 tccaacactg tttttggccc gtttctccac ggtatcagtg tggcgttcag ggaatttttc
     3721 ctaagccttt cgtaagctgt tttcaggtca aagtttctca accctttgtg ccatgcatcc
     3781 gctatccagg aagcggcaaa attcccggtc attgccggcc agtcccccgt cgccaaggca
     3841 aatgagggta tatatcagcc ctgcttgtac atttggatat aggagtttat ctggttgatc
     3901 tctctatctg gattcagtat catgtgcaga ggttccagcg ctatgtaatt atcccaaatc
     3961 caattgtcta cgtaaaaagg ggtgtcggat gaatgaatct tattgtcgta agcactgtaa
     4021 taacttccgt actcattgat atcaaccatt ctttcgtaag agcggtataa agccgtatag
     4081 aacactcttt tttgtgcgtc agatccaccc ttgacctgta tttgactcaa tgtctggtcc
     4141 caaaccgcat aggcattctt ttttactttc tcgaagtccc attccggaat ttctttcaac
     4201 aggttttttt tcgcttgttc aatgctgata aaggagattc catatttgaa ggatactttt
     4261 ttgttttgtt tcccgacccc gaccagcaac cacattttat ccgatgaatt cctgtattct
     4321 ttggaaacaa tatccgtatc agtttctccg taaaagaaga tttttattcc ttcaaactct
     4381 tcgaatcccg aaacatttct ggagttgcta acccatattt ctcctttgtc gttgaaaatg
     4441 ccgaacctta aataatgatc catgttattt ttgaaatgaa ccttgaaata accgctccgg
     4501 ctttgaggtg agaattcaag caaatcgcct gattcctcga aggaggtggt gtagtagtag
     4561 gggctgagct tttcctgaaa ataagttaac cgggtgttcc atgtttccgg attcttttca
     4621 ccgcttaacg gcaggaaggc aaaagccaag tgccttcgat gagaagtaag ggttaaagga
     4681 aaattaccta tttgatcatc gagatggtcc cttcgttggg ggaaaaacct gagcatgctg
     4741 ttgggcaggt gcactgtcgg cctcgtaggc tctaaaataa ttccgacacc gccaatcgac
     4801 gggtctacgt agggcaggtt ggattgggaa aagcctttca aaaaaaggaa aaatagagaa
     4861 cttaaaaaca gatacctgaa catacttgta attatttaac gtttgacgtt tatagaactt
     4921 ttcacctttt cagccaattc ctgatcaaat accggatcta ttccgcacac tttttgcaat
     4981 gtataatcga ttccctgaga gaggagtttt tcaaaaacga tgtcctgttc cgacctgtgg
     5041 ccgttttcat ccgttgcttt aaagaaaaaa gcgtacgaca tggcttccag gattttgtca
     5101 taaggcattc tcaacgggat ggccagacgg attattccca taaacctgtc atcggggcct
     5161 aattttctgg gaatatcctg tccaacccgg aatagggtgt ctttcaacga cctgttccga
     5221 aacctgaata gtaaatcctc aatgtgaacg gatagatctc ttggtgtaaa atcgtctgga
     5281 tatattttca aaagaacggc agcagactga agcatagcct cccttgtaaa ctctattacg
     5341 tgtgtgtctt ccaaaacttc atagaggtag gtagaaccgg gatatttaaa aaaaccataa
     5401 taagcagcag tggcatgtcc caaattatga ataaatgctt ttctatcgac ccatgccctg
     5461 atgttttcct taggagccaa ccccatgacg tccgggactt cgttcctgaa tcctttttta
     5521 tccaggatca actggttgta aggctccgca aagaccgata gaggatcttt ctttatctct
     5581 ccggcagtca taaggggaac catttttcca atacttgttt caaccagtcc aacataatcg
     5641 tgtagaggga aatcgtccgg gagattgtct ttaagttctt tatagatcac ttcatttgca
     5701 gaacgcatgt tctcagcaat gatgatatca accggaccat tttcagaacc ttgaaaacgc
     5761 tttattaatc ctttggcaag tgtgggaata attttcggca acgcattctt gccaaccgat
     5821 accgctataa tggaagtgtt ttttacttcc tcggtcacag cttcctgatt ggtaccgtta
     5881 attgccctta cgttggatat ggttatcctt tcctctttgt ctcctttaat cacgacggca
     5941 tatgacttct cgtggtttaa taatgaaacc agttcctcat cgatatcgga aaagacaact
     6001 tcgtatcccg acaaaccaaa cagttgcccg ataaaagagc ggcctatctt gccggcgcca
     6061 aaaattagga tggatttttt ttccataagt cgttcgttat ttaatctgct ttttgtagtc
     6121 gttaagaacc ggcgtcataa gttcgtattt ttcacccgga tatttttcaa aataggtgcc
     6181 gccaatatat aagcatgcgt aacctcctga tactatgccg agtgtggtgg ctgcctccag
     6241 gtcaagtttc atctttccct cgttgagttg aaacatcaac gagccaatca cgccgcctac
     6301 gaaattatct ccgcagcctg ttgtgtctcc gttataaacc ttttctctta ttctcttcga
     6361 tatagcgtcg gaaacgggta attcccgcat ttcaaccctc ccgaacaatt catttcctgc
     6421 atacagcaaa acattctttg ccccgttggt aatgataact gcacctgttt gcttctcaat
     6481 aaaaaattgc atggcttttt caatactttt ttccccgctc aagcgcaatg cctcatctct
     6541 atcggtaatc aaaagatcga tgtttgggta gctttcatcg ttttttccca atggccacct
     6601 tttatgtgga tttgcctttt cattcacgaa gtcgaaaacc gtgttaacca gtgtaatgca
     6661 tccgttctcc ttcgattgtt gaagcaactc aaccagattg tcatgtattt gcggcaccag
     6721 agcgctccct ccgaatacaa caatgtccga tttgaaaaaa tcgttatcca ggttttcggg
     6781 agaatagtcc caggccgagc ctatagagtt tacaaatacc ctttctccgt gcccgttatc
     6841 gtaactgggg tcggaaagga catcggttga tggagtcagg ttccccattt gcttgaaatg
     6901 actgatgttt accggaagtt tttgcaagga ggataacagg aaactcccgt tttcgtcagg
     6961 cccgtgacac ccgtagaact tgatttcact attcaatcca aatgtgatct gggcagcatg
     7021 gataagggct acgatgcagg gtcctccaac attgattttg tccggcgctc tccccgctgt
     7081 cagttcgttg aggataactt gaaaattctt tttcgtgtac tcttcaaatt cctctttcag
     7141 taccaggtgt ccgggactaa ggcctccgtc tcctcttttt ttcgaggaat aagaaagaaa
     7201 agcattggaa taaaaagata cgttgttgta cagcctgtcc accaggcagc atcctgtacc
     7261 ggatactgtt attttctttt taagcattcc tgttttacgt taagagagaa aagtgttgta
     7321 atcgttgacc aataaataga gtggcttttc atcttcgaca atagtcggaa atcttccgat
     7381 tatttcgtgg aaacagttat cagccgaatc atcgtttacc atgcttacct ccccgacaag
     7441 taccttccct ttccccggct cgccatagaa gcgatgatac ataccctgct ccagagaaat
     7501 actttctccc ggtgttaaga ctacttttcc tcctgcttcc acccaccctt ttataccgtc
     7561 tttcttaaag tgtacgtccg tggtatcaag ttggttgtca agggtagaat tgtaaagctc
     7621 gataaccagg tttcctccgc cccggttaat gatgtcttcc atttttgacc aatgatagtg
     7681 catcggggtt tcctgatttt cctccacgat cattattttt tcggcgtacg gctttttgtc
     7741 tacattgaat tttccgtttc tgatggtaaa cagaaacaat ccccggctgt gaaaatctcc
     7801 tgaaccgaag tcggttatgt cccatcccaa catgttgttg aaaatttctt ccgcattttt
     7861 tttgttttcc ctccactcat ctatcgtcca ataagcccag ggaggtaaca aaaaattttt
     7921 ttcgtgtaaa aactctttcg cttctcttaa gatctgattg atttcacttc gtttcataaa
     7981 tataaatttg atgttttagt agattgtatc tttcaaaagg gttttcccca ttttttattg
     8041 ggataaggac ccagttccaa ctcaagttct cctccgttta aaaaatcagt aacaggaaat
     8101 tgaaaacggg accattttat accgttcaat atggcgtgtt gaatgtatat gtctgcagga
     8161 tttttctttt ttacagaaat cctgaacagg gtgttttgta ggggatctgc caaagaggga
     8221 aacctcaaca ctattttatc gaacaggggg cttgtgatct ccaactcggg aaattcaccc
     8281 acacatccgt gcaaatcgaa taacccgatg gccattaaca ccccaagcgc acccagcatt
     8341 ccctgatctt cgtcaccgtt gtacccgctg tacggatcca ctcctccaaa agctttctcc
     8401 ttgacctcgc gcacccagta ctgtgttttc cagggagctc cggcgtgact gaacaaatga
     8461 gccattgcca gtgaaggttg gttctcataa tcaacccagg cagagccatg ttctccgtgg
     8521 ggagcaataa accgaaaatc ctttgctttt aaaaaatttg actccagctt ctcgatgaat
     8581 tttttgtttc ctcccatttc ctggattaaa ccttccatgt tttgaggaac ataaaaagta
     8641 tatgtagccg aacttccttc aataaatccg ggggaattga atttcccctt ttccacaacg
     8701 ggcgtgaaat ccttcagcca ttcccctgag acatgtcgcg gacgtgacca cccgattgcc
     8761 ggatcgaaaa catttctcca attctcggaa cgttgtatga aaagtttcct gtcttcgctc
     8821 tttttcagta tcccggccat ctgggcaaga caccagtcct gataagcata ttcaagcgtc
     8881 attgccgctc cctcgcggtg aaagccctca cctcgctccc tgatctcgat gggtacatat
     8941 cccttttcga cgtaccaatc catcccgcct cccgaaggat gcgggcctgc ttcgtaaccg
     9001 cccctgtccc ttatccctcc agggaaagca ttttttcggg ctccggtata ggcactaacg
     9061 ggatcgaatg tggcccgttt tgaagacaac agggcggcta tcaatggggt tgtgtgatct
     9121 cccaccatga cataggaata atttcccccc caggaacagc gggccattgt ccccgcattc
     9181 cggtagtagt caagaaatgt ttctaccatc cagttgccgt attcgggata aagcagtgtc
     9241 caaaagacgt tcaggttcca ttgtcctccc caaagcccgt ctccttcgat gaagtgccgg
     9301 gtgggcttgt tatcgcttcc caacgggagt tttcttgcca ccggatacat gcctgtccaa
     9361 tccatatatg aaccatctac atcgtcggat attctttttg aagcggtatg catcaaatcg
     9421 gtatatagct ttatgcgctg ttcacgggtc cctccctcca ccagtacccg gcttaaatag
     9481 ttgtcccacg cttctgcggc atcttttacc actttgtcaa aattccaatg cggtaattct
     9541 gagctcaagt tcatcctcgc attttcagtg ctgacaaaag agatgcctac cttgatgcat
     9601 atctgttcgt tgtttttcaa tcctctgtag gttacgtatg cgccgatggt atcgccggat
     9661 atgagatttt cttcagggcg gattatcttt cggatacctt ccttgttttt ctcccaaccg
     9721 ttaaaactgt caaatggctt gtcgaattga gcaacaaaga aaatcaggta aggttttttt
     9781 ctcctgaacg tgggggagag gagagaatat ccttcaattt cgtttgaact gatttttctt
     9841 gcgtaggcat atgatgtttt tgttgccccc agagcagcgc ttaaatcaaa gatgaagtgg
     9901 gcggtatctg ctgccgggaa agtgtaccga tgcattccta ccctacaggt agcggtcagc
     9961 tccgccgtaa tttgagaatc ttcgagaaaa actttgtgat aacccacctt gaccacctct
    10021 ttgtcgtgac tgaattccga cttgcttgct tcaagcccga ggtacccttt acattctccc
    10081 acaaccggca ttaccggtat accggcagtt tgtccgttgt gcacatgtga gaaattcagg
    10141 atatattttt cgtcgtataa ataccctgaa ttccacatct cgccgtgttt catatcggga
    10201 gagagggaga ccatcccaaa gggaagtgcc gcagatataa aataatcgta cctggatttg
    10261 tgcgtgtcaa tcaaagggtt tgccaaatct gaaggccttt cctgactcac ggaacggagg
    10321 tgaggaacgg ccataaccga tagaataagt aatattttta acctgttcat gttgatcttt
    10381 attgcgtgct gactttttgt catcccagat aatgtctctt gaacttatac cggatgtttc
    10441 tgtctgttct gcctctttcc ctgataacca ggtttgagtc ctccaacctg atgttatcta
    10501 ctccgttgaa atcttcaggg atcctggcca gaaaggccag aaaagtcttt tcaatcgttt
    10561 cttgggcatc gataaactca tacaggttcc ggtcaaagat cttccctttt tttgccacta
    10621 ccgtgaaagg ttcgtgtaac ccggtagtgc caaattccat accgaaagct acaggattgc
    10681 cgttttccat ggatcgccaa aaattaatcc agggataatc ctcaactttc cacagatagc
    10741 ccaacataac ccccagggtg ggattacttg cggtaaccca gccgtaagtt tcacgtctgt
    10801 tgtaaatgaa agaggatacc ctgggccagg gatcctgaaa atgccgcagg tctactttcc
    10861 catccttatg aacggcttca ggccaacaca gaacaggttc ttcctgatgt aaacttccgt
    10921 cttccttgtc ttcaaatcct ttttcggtat tgttatcgaa taaggttttc ctgtcgagaa
    10981 aaggcggcgc aacggtgacg tgttgaacta ggttgaacat gcggccatac ttgttgaggt
    11041 tcctgatttt ttccgaaacg aaaaatagcg gctcttctcc cgacagactg acctgccggg
    11101 ttatttccag tccccccatc ggtaaagtgc aacacataac gcattcgtta gaagtgctgg
    11161 tagcagcatg gtttattagt tcccatcgtt gactgttcac ttctccgtga tgcgtaaacc
    11221 catttgattt ttcggcttcg gaaggaggcc cccaacggtc gaaacaaagg aaatggcctt
    11281 taaaaggagg ttgtgtctcg tctttaatca cccagttaat gggattcaaa ggctgatctt
    11341 tcaggtgaaa atcggaataa gaaccgccat ctaaatttat cttcagcaag acgttgttgt
    11401 tttctaaaat gaccatgctt atagattatt tgtccggtat caacttttta ctcagcgctt
    11461 ccagggagat cccccgggtt tcaggtactt tctgtaatac ccatatcagt tgcagcacca
    11521 tcatgaaggc aaagaaggca aatatgggcc aggggttttc ccggaagaca cctctttcgg
    11581 aatccaaaaa aacaggagtg atcaacgtaa tgagggcagc aaagacccaa tgtatactcg
    11641 caccgaaaga ctggcctttc gcccggatac cggttgggaa tatttccgaa ataaagaccc
    11701 agataaccgt gccttgtccg atagcgtgcg atgcgcagaa caacaacaaa aagaccagca
    11761 ggaccatgga tccggcgtga gtatagaaac tccacgcaac cattacaagg cttataatgt
    11821 atccgaatga gccgataata agtaattttt tccggcccag cttatcgata aggtataatc
    11881 ctaaaaaagt aaaaatgatg tttattccgc caagcaggat ggagttcatt aaggagtctc
    11941 tagctgccaa tccggatcgt tccagaatct cgggagcata gtagagaata aaattaatac
    12001 ccgaccactg attgaaaaaa gcgatgaaga aagccaacca caggatacgt ttgtaccggg
    12061 ggataaaaag ggaagatttt ttacgcttca tttgttccct gatatggttt gcaatggttt
    12121 gaacctcttc tttcggatga ttaatcccta gccgggtaat tatttcttca gcttctccct
    12181 ccctgttttt catcaccagc catcttggac tttcgggaac aaagattacc agcagtgcgt
    12241 aaacaagaga aggtataatc ataacaccca gcatccaacg ccaatcgttt gctccgtcaa
    12301 atccctttaa aaaataattt gaaatatatg ctatcagcaa accaaataca atgttgaact
    12361 gatacattcc ggtaagcttt cccctgtttg ccggagtaga aatttccgat atatagatgg
    12421 gggccacaac agaagatatt ccgatcccca atcccccgat aaatcgaaag aaagaaaaag
    12481 aataggggtt tgtagccaat gccgttccta ttgttgataa gctgaacaag atcccgacgc
    12541 tgagcaatac tttttttctt ccgaattttt ccgtgggaaa cccaccgaaa attgctccga
    12601 tcacggtgcc ccataaagcc atggacatga taaaaaatcc atgaaatagt ggggaggtgt
    12661 tccacagttc cttaatgggt aaattggctc cggaaatgac aaccgtgtca aacccaaaaa
    12721 tgaaaccggc caaggcagct ataagggaaa taccggttaa attaattctc ttttcagaaa
    12781 ctactgtatt taggttttgc atacatcact aatttaagtt tgtccgttaa ggtttgatac
    12841 cgattgttgc aatttcatgg ggttttaact gtacagatag ggttgtacct tgtaaggcag
    12901 gtgaagatac accttggagg ggcatttcta tcaggttcag ccttcgtgcc gatgttgccg
    12961 caacgggtag attaatagtg gcggttgttg cttttccttc agtttccacc attctgatta
    13021 taagttcatt gccctcttcg gattgtttga tgcctgttat tgctatgttg ggctgggcta
    13081 cggagaagaa cgatttttcc tcgggttgtg tggcgtggtc ttttatcaga gccagcgagg
    13141 gaggctctgc tgccagcacg ggaacattgt aatcatcgcc ttctacccat acgccattct
    13201 tccaatctcc cgcgtgagga taaagtgcat aactgatgac aaatttgccg agattgggat
    13261 agatgtccgg actgcctgcg gctctcatca gcgtaagccg tagttccccg tcgtgatagg
    13321 aatgcccgta tttggtttta tttagaaggg ctataccggc ttgcccgtcg gttacgtcca
    13381 cccatttttg tgcgggcact tcctgtccgt cacctctttc gggatcaaca ccgtaaacat
    13441 tcgattggga ggtgaggtgt ctgggcacgg ctcctccctg aaacttaccg tttgccggcc
    13501 tttcaaccac atcaaaaggc acctggcaat aaaatcttgg agcatcaaat gccagtggaa
    13561 agtgagccct tagcatgggt gaatcggttg agtctgttcc ggtttcaagc caatgcactt
    13621 ccatttcgta ttcgattctc ggataggaac ggtagatgta ggttctttcg gtgaaactcg
    13681 attttcccca gtatttaacc gtttcaatac atgcccttac cggcccgttt tctactacct
    13741 tcacgcttct tacatcatct acctcttctt cttttacaat cttattcaag gtccatgatt
    13801 tcattccccc tttcttgtct tctagataca ttttgaggcg attcagttct ttcccttcct
    13861 gaacatattc tttcccgtta cgtttgtcgt acagacttga gattgatccg gttttcatgt
    13921 ttacttttac ctcaaagaaa tctgtttcga atgtgttgtt ttcgaaaggg atcggttcat
    13981 tgaacgtgcc cggctgggtc atatccacgt agaacgtttt gtatcctcct gccggcattt
    14041 cgtcaacaat gaattgtatt tttgaggtga accccggagg agttaccttg gaccatacaa
    14101 tttgcgcggg atacgttttt ccggaagcat cacgaaccaa aacagtagga taatttccct
    14161 ggcctttatc gatgggagta atgttttttg acccgtaata atcgccccag cttgtcgatt
    14221 ttacggaagc aggttgcgtg tgagaataga cgtttgcttc cacaaccgtt ttacgcgaga
    14281 ctggctgcag gttaaatgct accacaggtt gtcccatgtt ggtctgaaat tttatttcgt
    14341 cggctgtttt ccggaaggcg ttgtccctca gttccgtcga cttccggaga ttttccatat
    14401 accgggcaac ggcttctttg ttggcttcgt tgatagccga tccgggaaga atatcgtgaa
    14461 actggttgaa tgtttgtgta atccagaggt cgcgtatttc ttcagcaggg tagttgtctc
    14521 ctttcaacca gcgtagcgta ttaaaaaact cgttgctgta cagcatattc tcgttgttac
    14581 ggttcccgga ttttatttcc gatacggtgg tgtaacaacc ttcgaatata aactgcattt
    14641 caccgtgatg cgttggtttg tctttggctt caacggcgct tttcttaaag aaatctttcg
    14701 ccgtcgtaaa ttttacggca ggataatcag ccatcttatc cagttgatgg accatctcta
    14761 tgtttgcccg ggtgggacct cctccgtgat ctcctacgcc ggtaatttgc aggatccggt
    14821 gccctttggg gtcaaatttg gtgagttcat ctttcagttt caagttaatg tcgccgttat
    14881 aggtgtcgtt tgcgtagcaa aggactttag ataaatcagt cccttcccac cagaatgtac
    14941 cgatataagg tttgcaccgg tgaaaataga aataattaca tccggccaat ttcaatattt
    15001 gaggcatttg cgacacatgg ccgaagttat cgggtagcca gccgatggtt gccatttttc
    15061 cgaaatgttt ctggaagtag cgttgtccaa gtaaaaagga acgggttatg gcctcaccgg
    15121 aagaaagatt catatcgcct tcggtccaca tacccccaac gggttcaaag cgcccctctt
    15181 ttacgtagtt tttaacttct tcaaacaacg gaggatctac tttttccacg aagttgtaaa
    15241 cagaggcctg gctctgaatc atggtataat cgggaaattc cttcataaaa gcgacggctt
    15301 gcctgagatt gtcgttgcac attttcatgg tttcggagta agtccatagc cagttcatat
    15361 ccatgtgggc atgtcctacg acatggatgg tatctttctt tgatacgtcg ggaacaagag
    15421 gttcgttgaa cattgcaaaa gaggataacg atacccacga aacacaaagg gtgataagaa
    15481 ttgctttcat cgtattcatt ttttgatgat ttagtttaga aaaacattaa atttccactt
    15541 caataaattt cacttcataa ggttttaacc ggatggctga tatcggttgt ccggttctct
    15601 ctcccaggga attaatttcg gttacttttt ttatggttag ggtttgatca gcgggtgaca
    15661 gcttaatatc ggccggcaat ccttcgagtt cacgtaaatg cagtataatc gattttttgt
    15721 gaaaggacgg gcggatattt attaccaaga cgttttcggg gccatcgatt tttaatgttc
    15781 tgagcgatgc attgcataat ttgttctctc ctgcgggaag ggttctggtg gcaaaaggat
    15841 ttcgttctcc ccatgcgaat tttgtagcaa aagcgttggt ggtatcgttt gtagaggtaa
    15901 gttgataact ccagctaaaa gttccttcct ggtaagccct gaaattcgta aaccagtaat
    15961 tgttcattac ccaggaatag agccagggaa gtccttgttt gggatagcgt tcgaatttac
    16021 ccatgttgaa atcgctgaat tgccacaggg gaacttcatt actgacaact accacctggc
    16081 ctttgctgct ccgaacggaa acgaagtttt gggcgacatt ccagtcggaa gaagagcccg
    16141 gaagctgctg gccctgggtt aatatgcctc c
//
LOCUS       DAOSDR010000012        16066 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrimonas sp. isolate AMR_MDS_1826
            NODE_1468_length_16066_cov_4.374305, whole genome shotgun sequence.
ACCESSION   DAOSDR010000012 DAOSDR010000000
VERSION     DAOSDR010000012.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863355
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrimonas sp. (wastewater metagenome)
  ORGANISM  Petrimonas sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Dysgonomonadaceae; Petrimonas.
REFERENCE   1  (bases 1 to 16066)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16066)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:10:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,720
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 35
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16066
                     /organism="Petrimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1468_length_16066_cov_4.374305"
                     /isolate="AMR_MDS_1826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2023866"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..807
                     /locus_tag="PLI30_01010"
     CDS             <1..807
                     /locus_tag="PLI30_01010"
                     /inference="COORDINATES: protein motif:HMM:NF016648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR domain-containing protein"
                     /protein_id="HOI78164.1"
                     /translation="IFGKIRLETTREKSYLSVNWRKAMQWAAIFLLPVLSAFTVYYFT
                     QRPQRNLQPTVITAQKGEKAEVVLPDGSNVWINSGSTLTYDRHFNGKERSVYLQGEAY
                     FEVTENKKRPFIVHTKHITVQALGTSFNVRSYETDSLASAVLLEGKVKVSASGHETIL
                     SVNERATFDKRKQTLLADRVEALNFIEWKNGNIYFSNQTYDEIARALSRIYNVEIQFA
                     SEQLRPMRFSGTLGSSGIKNALDILSMTSPMYYEMKDTTIILHHKAEMVK"
     gene            936..4346
                     /locus_tag="PLI30_01015"
     CDS             936..4346
                     /locus_tag="PLI30_01015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006843616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0071702 - organic substance transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SusC/RagA family TonB-linked outer membrane
                     protein"
                     /protein_id="HOI78165.1"
                     /translation="MNQNLLRRKGKTLCSAKFLVAFIFMLFFLPSFSQITVNVQNKTL
                     RQALKEIEKVSNYRFFYNESLAILDKNSSIQLTNSSIDKAMQTLLAGTDVAYEKNNHI
                     VVLVDKGTVALQPGETQQDKVTVRGRVLDEAGVPIIGANVIEEGIAGNGTVTDYNGNF
                     ILTVSSKAKIKITYLGYGDVVLNTAGGTSFNVVMQEDTRTLNEVVVTALGIKREEKAL
                     GYSVQAVEGDGLQTVKGVDMGTSLTGKIAGLLVRNSTEFSDAPSIQIRGEDPLLVIDG
                     VPYANMTLRDVPSDDIESISVLKGATASALYGYRGGSGAIMVTTKKGLANKGLSVSVN
                     SGTMFSAGYLAIPELQSDFGRVVRKNADGALEYVRSGDGSWGVPLDGREIIQWDPISK
                     MMKPMPYLPVGKDNFQNFLEQGYILNNNVNLAQQGEFGSFRTSLTWVNNKGQYPNSTF
                     DKLTFSLGGDMKIDKFLLTASMAYNKQASPNKGFSGYTGYDPMYNLLIWSAPDYDIRD
                     YKDYWLIKNEVQNSSYTSTNNNPYFDRYERTHSINRDIFNGSVSLDYDFTSWLKGTVR
                     SGFDIYSDRQDITVSKGSFQGAGSATVLAGGTQVWGESQRGSYNIGLGRGYSLNNDLL
                     LSADKSFGDVRMESFIGGTIYYTQNDGMEARTQGGLTIPAFYSLKASVNPAAVSSVMY
                     KQQVNSLYGRLALSWKSLLYGEATLRNDWSSTLSETTRSYLYPSLSGSFIVSELLPKY
                     NWLSLWKLRGSWTASKTPAGVYSINSVYNITSNAWGNLSSAAYPSAIRGTDVLPESAS
                     TFEIGTAVNVLKNRVSIDVAFYQKRMYDFLRWAPISPASGFTSNYINIDEEITRKGVE
                     VTLNATPVVTRDWQWDVSLNWSKYARYYTQLDSVYSTDRPWVKVGERADHYVLNDYQK
                     DPQGNIIHNNGLPLYSSYQSLFGYYDPDWLWGISSSLKYKDFTFGISLDGRVGGIAQT
                     TTEMYMWRAGSHPQSVVAERFLDANQPGTKNYVGQGVKVVSGSATYDTYGNITSDNRT
                     YAPNDVAVTYQTYINNLHRGTAWGGSPSTLDAYSTTFFKIREVSLSYTLPKSVVEKFR
                     ANNATLSAVGQNVFLWAKQFKYSDPDGGSDNFSDPSIRYLGFNLRVVF"
     gene            4474..6006
                     /locus_tag="PLI30_01020"
     CDS             4474..6006
                     /locus_tag="PLI30_01020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006843617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SusD/RagB family nutrient-binding outer membrane
                     lipoprotein"
                     /protein_id="HOI78166.1"
                     /translation="MKKNYSNIRKFTVFVLSAFTLLSCNNFEEINTNPDDTTSVSASL
                     LATNVILRVTKFAGRDGKSLIADNALPKYVGYANEGQMDQQYNKIGSSDFGGMTILPN
                     IEKMIEYAEGSVMEDSYRGLGAYAKAYTFFRLTMEMGDIPYSEAGKGAQGVYKVKYDT
                     QESVLKGILDELQAADQYFAKGRTFEGDPTPYKGDPVKWRKAVNSFALKVLMTIGKKE
                     SVGGINVKSRFAEIVSAGNILDGSTGFLGLEYSSQNKHPLSGTNDLFTSRTVISSLLI
                     DNLKKLNDRRIYYFAEPAGAQIAAGKTKSDLEAYVGVDVEIEYAVMNSGHSNNLYSLL
                     NKRYLQEEATEPLRIVSHAEQQLILAEAVLKGWISGDAKSYYESGVKSALADVMKVNK
                     NYAQDMPIDQAYIDGYFTGEAAFKTTLQEQLQQIWMQRYILQFMQDAETSFFEYRRNG
                     YPVFPINAATSLNENNKNGLPMRWLYPGSETTYNRENLIEALNRQYDGYDEINKIMWI
                     LK"
     gene            6018..6866
                     /locus_tag="PLI30_01025"
     CDS             6018..6866
                     /locus_tag="PLI30_01025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 16 protein"
                     /protein_id="HOI78167.1"
                     /translation="MTNKTILLIFLSGIFFFACQSSKQEKQPEQFETISTPTGNWQLI
                     WNDEFDYNGLPDSAKWSYDTDGNAWGWGNNEAQHYTANDTANAHVKDGLLTITAKIDS
                     AGGKRYTSARLITKGKGDWLYGRFEIKAKLPTGLGTWPAIWMLPTDWEYGGWPDSGEI
                     DIMENVGYDPDTIVGSAHTKSYNHVLGTHKNGKIYVPDSYTGFHVYTLEWEEKEYRVY
                     VDDQHYFTFVNEGTSFAEWPFDKRFHLLLNLAVGGNWGGQKGIDDSLFPHHFYIDYVR
                     VYQKAE"
     gene            7097..9355
                     /locus_tag="PLI30_01030"
     CDS             7097..9355
                     /locus_tag="PLI30_01030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019538192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HOI78168.1"
                     /translation="MDPIYLIIVIILLGLAILDLSVGVANDAVNFLNSSIGSKVAPLW
                     VILTVASVGVLLGTLFSSGMMEIARSGVFHPEMFSFPNIMVLFLAVMLTDVILLDVFN
                     SLGLPTSTTVSLVFELLGSAVAVAIFSIWQANAGDLIEYINTGKALAIISGIFVSVAV
                     AFIVGSSVMYITRIIFSFKYKRTFKFFGALWGGFALTAMTYFIVFKGLKNSPIVATDF
                     LNLVESNMLKSLLVTFAGWSVFMALLQFLFRVNILKIIVLTGTMALALAFASNDLVNF
                     IGVPLAGLDAFKNASATLASGSSIDTLYMDALNGPVKADIRMLLAAGIVMVLALWFSK
                     KAKTVTETEVNLARQGSGLERFSSTPLSRSIVRWGVDTNRFLNKLTPSSFREKLNNRF
                     VIPEQIEEQGVSFDLIRASVNLTIAALLISLGTSLKLPLSTTYVTFMVAMGTSLADRA
                     WGRESAVYRITGVLTVIGGWFITAIVAFTVAMVVAMFLAWGGKIAIFIALALVGFILF
                     KSSKFHQKRKLAEKANATLIEDESGIVKTSTTEVREVIGKMLDIYHQNVKGLTKESRS
                     LLREIASKADELYLNYKNKRTYEVVPTIQSITLKELDIEQEYVQIVDYTYEITKALRV
                     ITSDSSLYIENNHKGFNDEQEEDLEDLSKKVTNMYKTFIDMMERNDYSGFATIINIRD
                     EIIEQCAKLTKKQIRRVKDKESGTRNSILFMNILNETKTIVLQSVNLMKSQRNMILTV
                     KKISEEVTEKKN"
     gene            complement(9352..10143)
                     /locus_tag="PLI30_01035"
     CDS             complement(9352..10143)
                     /locus_tag="PLI30_01035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007574090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HOI78169.1"
                     /translation="MLITSLQNPSVKNIVKLAKSRERKEQQLFVIEGARELSLALQSD
                     YIVESVYVCWEMFEKTKYPGVLDSMEEKNVLDISPEVFGKIAYRENSDGVVAIAKPKL
                     HTLEYIKLSGNPFVIVLEAVEKPGNLGAILRTADAAAVDAVVVCDPQTDLYNPNVVRS
                     SVGGIFTVQTAVCSSADAAAWLKANNISSYAAELQAAEFYQDIDFRMPSAIVMGTEAE
                     GLTGFWLKNATKRIKIPMRGKIDSLNVSVSTAVLTFEAMRQRGEG"
     gene            complement(10153..10620)
                     /gene="trxA"
                     /locus_tag="PLI30_01040"
     CDS             complement(10153..10620)
                     /gene="trxA"
                     /locus_tag="PLI30_01040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002561278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HOI78170.1"
                     /translation="MQSIKMRKLTVIVAVFALVLSAWAAEPQVKKTVKPIALNTTSFT
                     EKVFDYNNEKEWKYKGDKPAIIDFWAAWCGPCRQIAPVLEELAAEYGEEIYVYKVNVD
                     EETELARAFGIQSIPTILYVPMTGTPQGVLGAAPKSQLKKAVDTLLLNKEEGK"
     gene            complement(10705..11208)
                     /locus_tag="PLI30_01045"
     CDS             complement(10705..11208)
                     /locus_tag="PLI30_01045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006843280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HOI78171.1"
                     /translation="MKTFSIEERERIETVIRSCKVCFVGMADEEGRPYVLPMNFGYED
                     GVIYLHSAQEGRSIRTMEKNPRICVTFCSDPELIRQHPDVACSYRMKAGSVICEGEVE
                     FEDDFAEKEKALHIIMRQYVDKKFTYSVPAVNNVKIWKLRMDSVGAKEFGIMRPGSIS
                     YKDRMEF"
     gene            complement(11215..12348)
                     /locus_tag="PLI30_01050"
     CDS             complement(11215..12348)
                     /locus_tag="PLI30_01050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005834653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HOI78172.1"
                     /translation="MHKQFQVKTILQNLLTVILFLLIFPGCIAPGNKSSGPVRYTNPL
                     PVALGDPFILSASDGKYYMYGTGGVEKGFGAYSSNNLADWQYEGQVYSGDTPESWTLK
                     NYWAPEVYEIDGKYYMFFSADWKVNPTNESENFRIGVAVADKPIGPFKELSNKPLFDP
                     GYPIIDANILKHKGKTYLYYSRCCYKNPVESEVAEWARSQNLFDEIEESWVYGVEISN
                     DFTSVIGEPVLLLQPPSKMSDLQTEWESRSVTNGEVNRRWTEGSFAFEHGGKIYMMYS
                     ANFFGGQNYAVGYAVSDNPLGPFVKAGNNPILEKNRDGGGNVTGTGHNMMFRLKGSEK
                     MYTVYHGRTRKTGEERVVFIDELKMDEKGVLSVDGPSTSEKEL"
     gene            complement(12341..13219)
                     /locus_tag="PLI30_01055"
     CDS             complement(12341..13219)
                     /locus_tag="PLI30_01055"
                     /inference="COORDINATES: protein motif:HMM:NF016296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family transcriptional regulator
                     DNA-binding domain-containing protein"
                     /protein_id="HOI78173.1"
                     /translation="MSRILVVFCRPHPGYANFKVYIATCTVIAAGVFLVLSFLQPFNL
                     GDRNILDNPFLTAALYAGGSYVTMLINFVWLKFFPRFFSNERWIVGKELFMCVYQMMT
                     IASTIWLINYIRGVFPPNIHGYLGMLWVVVSVGFFPCLTAQLIRHIYFMKKRLRKATA
                     MNISLMLDKKETPAEADPQFINLQRFIKPIDINTFISAESKGDYLVVNVSNNGSVEEL
                     TINATLEEFEAENTHFEQLFRCRDEFIINKNKITWVESNAAGYKLQLHPKLPSVFVSG
                     EKAVELKKRMDVEKYA"
     gene            complement(13409..13753)
                     /gene="rplT"
                     /locus_tag="PLI30_01060"
     CDS             complement(13409..13753)
                     /gene="rplT"
                     /locus_tag="PLI30_01060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005469617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HOI78174.1"
                     /translation="MPRSVNHVASRNRRKKVLKLTRGYIGARKNVWTVAKNTWEKGLT
                     YAYRDRKNKKRNFRALWIQRINAAARQYGMSYSRLMGALHKNEIDINRKVLADLAMNH
                     PEAFKAIVDKVK"
     gene            complement(13849..14046)
                     /gene="rpmI"
                     /locus_tag="PLI30_01065"
     CDS             complement(13849..14046)
                     /gene="rpmI"
                     /locus_tag="PLI30_01065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004584226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HOI78175.1"
                     /translation="MPKMKTNSGAKKRFALTGTGKIKRKHAYKSHILTKKTKKQKRNL
                     TQTGLVHKVDENSVKHLLGLK"
     gene            complement(14233..14793)
                     /gene="infC"
                     /locus_tag="PLI30_01070"
     CDS             complement(14233..14793)
                     /gene="infC"
                     /locus_tag="PLI30_01070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006798841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HOI78176.1"
                     /translation="MRNNFRDTKDQHRINERIRVPEVRLVGDNVEQGIYPTREALRIA
                     EEKELDLVEISPTAVPPVCRITDYQKFLYQQKKKQKEQKAKAVKIVVKEIRFGPQTDD
                     HDYNFKLKHAKSFLEEGAKVKAYVFFKGRSILFKEQGEVLLLRFANDLEDYARVEQLP
                     VLEGKRMIIMLTPKKQGSAKKEQPSE"
     gene            complement(14854..>16066)
                     /gene="thrS"
                     /locus_tag="PLI30_01075"
     CDS             complement(14854..>16066)
                     /gene="thrS"
                     /locus_tag="PLI30_01075"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019539980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HOI78177.1"
                     /translation="GKELELFTFSQVVGAGLPLWLPKGTQLRLKLEEFLKKIQQQFEY
                     EQVITPHIGGKQLYVTSGHYAKYGKDSFQPIRTPEEDEEFLLKPMNCPHHCEIYKAFP
                     RSYKDLPLRLAEFGTVYRYEQSGELHGLTRVRGFTQDDAHIFCMPEQVKDEFLKVMDI
                     IFIIFKALDFKDFEAQISLRDPENKEKYIGTDENWDKAERAILEACEEKGMKARVELG
                     EAAFYGPKLDFMVKDALGRRWQLGTIQVDYNLPDRFELEYTGADNQKHRPVMIHRAPF
                     GSMERFVAVLIEHTAGKFPLWLTPTQVVILPVSEKFNDYAYSVARTLKMQDIRAEVDD
                     RNEKVGRKIRDNELRRTPYLLIVGEKEAENEEVSVRKQGGIDQGSEKLTTFAHRIKQE
                     VDEMMNPQVNI"
BASE COUNT         4531 a         3665 c         3454 g         4416 t
ORIGIN      
        1 attttcggaa agatccgcct agaaacgacg cgagaaaaaa gctatttgtc ggttaactgg
       61 cgaaaagcca tgcaatgggc agctattttt ttactaccgg ttctttcagc cttcaccgtc
      121 tactacttta ctcaacgtcc gcaaaggaat ctccaaccca ctgtgatcac agcacaaaaa
      181 ggggaaaaag ctgaagttgt tttacccgat ggaagcaacg tctggatcaa ttcaggctcc
      241 accctcacct acgacaggca tttcaacgga aaagaacgct ccgtatactt gcagggagag
      301 gcttactttg aagtcacgga aaacaaaaaa cgccccttta tcgtccatac aaaacacatt
      361 accgtacaag cactgggaac ttctttcaat gtacgttctt acgaaaccga ctccctggct
      421 tcagctgtct tgctggaagg aaaagtaaag gtctccgcat cggggcatga gacaatttta
      481 tccgttaatg aacgagccac attcgacaag agaaaacaga ccctgttggc agaccgggtg
      541 gaagccctca acttcattga gtggaaaaac ggaaacattt atttcagcaa ccaaacgtat
      601 gatgagattg ccagggcttt atcgcgcatc tataacgtgg aaattcagtt tgcttctgaa
      661 cagcttcgcc ctatgcgatt ttcaggaaca cttggtagca gcggcattaa gaatgcactg
      721 gatatcttgt ccatgacctc tcccatgtac tacgaaatga aagacaccac aatcattctg
      781 catcacaaag ccgaaatggt taaataacat taaaatatca acctgcgtta gagtaaacaa
      841 ccgactcaac tgtattctgt ttataggagt tccctttatg tggaacttat tatttattgc
      901 gtagaatttt ttaatattaa ctcaattatt tgtctatgaa tcagaatcta ttacgaagga
      961 aaggcaaaac tttatgttca gcaaagtttc ttgtcgcatt tattttcatg ttgtttttct
     1021 taccttcttt ctcgcagatt actgtaaacg tacagaacaa aaccctgcgt caggccttga
     1081 aagaaattga gaaagtaagc aattaccggt tcttctacaa cgaatcactg gccattctgg
     1141 ataaaaacag ttctatccag ctaacgaact ccagtatcga taaagccatg caaaccttgc
     1201 tggccggtac agatgttgcg tatgaaaaaa acaaccacat tgttgttttg gttgataagg
     1261 ggactgtggc tctgcaacca ggagaaactc aacaggataa agtaacggta cggggccgtg
     1321 tacttgatga agccggggtt cccataatag gggcaaacgt gatagaagaa ggaatagccg
     1381 gaaacggtac ggtcaccgat tacaacggta atttcatatt gactgtttcg tcaaaagcaa
     1441 aaatcaagat cacctacctc ggatacggag acgttgtatt gaatacagct ggaggcacct
     1501 ccttcaatgt tgttatgcag gaagacacac ggactcttaa cgaggtggtt gtcaccgcac
     1561 tgggcataaa aagagaagaa aaagctttgg ggtactcggt acaggcagtt gaaggcgatg
     1621 ggctacaaac cgtaaaaggg gtggatatgg gtacctccct gactggaaaa attgcaggac
     1681 tgctggtgcg aaacagcacg gaattttcgg atgctccttc tatccaaatc cgcggagaag
     1741 acccgctgct ggtcattgat ggagttccat acgccaacat gaccttacgg gatgttcctt
     1801 ctgacgatat cgagagcatt agtgtattga aaggagctac agcctcggct ctttacggat
     1861 accggggagg gagtggagcc atcatggtaa ccacaaaaaa agggctcgcc aacaaagggc
     1921 tatccgtttc ggtaaactcg gggaccatgt tctctgccgg atacctggcc attcctgaac
     1981 tgcaatcaga ttttggacgt gtcgttagaa aaaatgccga cggagccctc gaatatgttc
     2041 gttccggaga cggatcctgg ggtgtaccac tggacggccg ggaaataatt cagtgggatc
     2101 caataagcaa aatgatgaag cccatgccct atttacctgt tggaaaagat aattttcaaa
     2161 atttcctgga acaaggctat attttaaaca ataacgttaa tctggcccag caaggagaat
     2221 tcggaagttt ccggacatcg ctcacatggg tgaacaacaa aggacaatac cccaactcta
     2281 cgttcgacaa attaaccttc agccttggag gagacatgaa aatcgataag ttcctcctta
     2341 cagcgtcgat ggcatacaat aaacaagcat cccccaacaa ggggttcagc ggttataccg
     2401 gttatgaccc gatgtacaac ctgttaatct ggtcagctcc cgattacgac atcagggact
     2461 ataaagatta ctggttaatc aaaaacgaag tccaaaatag cagttatacc tccaccaaca
     2521 acaatcccta cttcgacaga tacgaaagaa cgcacagcat caaccgcgat atctttaacg
     2581 gatcggtatc gttggattat gattttactt cgtggttgaa aggaactgtc agaagtggat
     2641 ttgacattta cagcgacagg caggatataa cggtttccaa aggttcgttc cagggcgcag
     2701 gcagtgctac cgttttagcc ggaggaacac aggtctgggg ggaatcgcaa cgaggttcct
     2761 acaacattgg ccttggcaga gggtacagcc tgaacaacga cttgctgtta tccgccgaca
     2821 aatcatttgg agatgtccgc atggaaagtt ttatcggagg aactatttat tacacacaaa
     2881 atgacgggat ggaagcacgc acacaaggcg gactcaccat accggctttc tattcgctaa
     2941 aggcatccgt gaatccggca gccgtaagct ctgtgatgta taaacaacag gtcaacagtt
     3001 tatacggacg gctcgcctta tcgtggaaaa gcctacttta cggagaagca acgctgcgta
     3061 acgactggag ctccacgctt tcggaaacga cccgctccta tctctatcct tcactctcgg
     3121 gaagctttat cgtttcggaa ttgctgccca agtacaattg gttatcgcta tggaagttga
     3181 gaggttcatg gacagcgtct aaaacccctg ccggtgttta cagcataaat tcggtgtata
     3241 acatcacctc caatgcttgg ggaaatttaa gttccgcagc ttatccttcg gcaatccggg
     3301 gtaccgacgt gctgcccgaa tctgcttcca cttttgaaat aggaacggca gtaaatgtcc
     3361 tgaaaaacag ggtatccatc gatgtggctt tctaccagaa acgcatgtac gactttttac
     3421 gatgggcacc catcagccct gcttcgggct ttacatccaa ctacatcaat atcgatgagg
     3481 agataacaag aaaaggggtt gaagttacct taaacgctac tcccgtagta acccgggact
     3541 ggcaatggga tgtatccttg aactggtcaa aatatgcccg ttactatacg cagctggatt
     3601 cggtttattc aacagacagg ccctgggtga aagttggcga aagagccgat cattatgttc
     3661 tcaacgatta ccagaaagac ccgcagggca acattatcca caataacgga ctgccacttt
     3721 attcgagtta ccaatcgctg tttggatatt atgatccgga ctggttgtgg ggtatctctt
     3781 cctccctgaa gtacaaagat ttcacgttcg gcatctcact ggacggacgg gtaggcggta
     3841 tcgcacagac tactacagaa atgtatatgt ggagagccgg atcccatccc caatcagtcg
     3901 tagcggaacg ctttctcgac gctaatcaac ccggaacaaa aaattacgtt ggacagggag
     3961 ttaaggttgt atcggggtcg gcaacatacg acacttacgg caacataaca agcgacaaca
     4021 ggacgtatgc tccaaacgat gtagccgtaa cctatcaaac ttacatcaac aacctgcaca
     4081 ggggaacagc gtggggaggg tcaccctcaa ccctggatgc atattcgaca acgttcttta
     4141 aaatcaggga ggtatcgctt tcctatacgc ttcccaagtc ggtggttgaa aaattcaggg
     4201 ctaacaatgc taccctttcg gctgtaggcc agaacgtttt cctgtgggca aaacaattca
     4261 agtattccga tcccgacgga ggttcggaca attttagcga tccctccatc cgttatttgg
     4321 gtttcaacct gagggttgtt ttctaagcag gtcatttata taaacaattc cttgtaaagg
     4381 aagtattgaa caagcatgta aatcaattac aactcaggaa atgatagagg caagttctgt
     4441 acaactttaa agaaaaaaat aaaatataat cgtatgaaaa aaaattattc caacataagg
     4501 aaattcacgg tattcgtctt atctgctttt acgttattga gttgcaataa cttcgaggag
     4561 attaacacaa atcccgacga caccaccagc gtaagcgcct cattgctggc taccaacgtt
     4621 atccttcgcg tgactaagtt tgccggaaga gacggaaaat cgctcatcgc agataatgca
     4681 ctgcccaaat acgtcgggta tgccaatgaa ggccagatgg accagcaata caacaaaata
     4741 ggaagcagcg atttcggtgg aatgaccatt ctcccgaata ttgaaaagat gattgaatac
     4801 gcggaaggca gcgtcatgga agactcatac aggggcttgg gagcgtatgc gaaggcgtac
     4861 acttttttcc ggcttacgat ggaaatgggc gacataccct acagtgaagc cgggaaaggg
     4921 gcgcagggag tgtataaagt gaaatacgat acgcaggaat cggtcttaaa aggtatcttg
     4981 gacgaactgc aagctgctga ccaatatttt gcgaagggca gaaccttcga aggagatcct
     5041 acaccctaca aaggtgaccc ggtgaaatgg agaaaagcgg taaattcttt cgcactaaaa
     5101 gtactgatga ccataggcaa gaaagaatcc gtaggaggta taaacgtaaa atcacgattt
     5161 gccgaaatag tatcggccgg aaacatactt gatggttcta ccggatttct cggactggaa
     5221 tactcatcgc aaaacaaaca tccgctttcc ggtacaaacg atttatttac cagcagaacg
     5281 gtcatcagct ccctgttgat cgataacctg aaaaagctga acgaccgccg catctattat
     5341 tttgcagagc cggcaggcgc acaaattgct gcgggaaaaa caaaatctga cctcgaagcg
     5401 tacgttggag tagatgtaga gattgaatac gcagttatga attcaggtca cagcaataac
     5461 ctgtactcat tgctgaacaa aaggtactta caggaagagg caacagagcc gttgcggatt
     5521 gtgagccacg cagaacagca gctcattctg gctgaagccg tcctgaaagg atggatttcc
     5581 ggtgatgcga aaagctatta tgaatcgggt gtaaaatctg ccttggcgga tgttatgaaa
     5641 gtgaacaaga actatgcaca ggacatgccg atagatcagg cttacattga cggttacttt
     5701 accggggaag ctgcgtttaa aaccaccctc caggagcaat tacagcaaat ctggatgcaa
     5761 cgttacatct tgcagtttat gcaggatgcc gaaacaagct tcttcgaata caggaggaat
     5821 ggataccctg tttttccgat aaatgccgcc accagcctaa acgagaacaa caagaacggt
     5881 cttccaatgc ggtggttgta ccccggatct gaaaccacct acaacaggga aaacctgatt
     5941 gaggcgttaa accgtcagta cgacggatac gatgaaatca ataagatcat gtggatttta
     6001 aaataacatt caatcagatg accaacaaaa cgattctact tatttttctt tccggaatat
     6061 ttttcttcgc ctgccaaagc agcaaacaag aaaagcagcc tgaacagttt gaaacaatta
     6121 gcactccaac cggaaattgg caacttatat ggaacgatga gttcgattat aacggattgc
     6181 ccgactccgc gaaatggagt tacgatactg acggcaatgc ctggggatgg ggcaacaacg
     6241 aagcacaaca ttacacggca aacgacactg ccaacgcaca cgtaaaggac gggctgctga
     6301 ccatcaccgc gaaaatcgat agtgcaggag gtaaaagata tacttctgcc aggctgataa
     6361 ccaagggcaa gggcgattgg ctttacggca ggttcgaaat aaaagcaaag cttccaacag
     6421 gattgggtac ctggccagcc atctggatgc tgcctaccga ctgggaatat ggcggatggc
     6481 cggatagtgg agagattgac attatggaaa acgtcggcta cgatccggat accatagtag
     6541 ggtcagctca cactaaaagt tacaaccatg tgcttggaac gcataaaaat ggcaagattt
     6601 atgtccctga ctcctataca ggattccatg tatataccct cgaatgggaa gaaaaagaat
     6661 accgtgtgta cgtggatgac cagcattatt ttacattcgt aaatgagggt accagttttg
     6721 ccgaatggcc tttcgacaaa aggtttcacc tcctgctgaa cctggctgtt ggtggaaact
     6781 ggggcggaca aaagggtatt gatgattcac tctttccaca tcacttttat attgattacg
     6841 taagggttta tcaaaaggca gagtaaaagc cactgaatta ttgcatttaa gaaaagagcg
     6901 ggagacttac aacgtttatc ccgctcttcc tttttcagta agatataaaa ttgttacatt
     6961 tttgttaagt ttttgatttc aagcataaat tgctcttgat ttttaaatca caaagaaaat
     7021 ctttcattaa ttttgtgcac aaaataaaag gaagaattga ggttttgctt caacaagtgg
     7081 ccaaaataaa atttttatgg atcccattta cctaattatc gtaatcatat tgctgggttt
     7141 agcaatactc gacttaagtg tcggcgtagc caatgatgcc gtgaattttc tcaattcaag
     7201 tatcggttca aaagttgccc ccctgtgggt aatccttaca gtagcatccg ttggcgtatt
     7261 gcttgggacc ttgttttcca gcggaatgat ggagattgcc cgaagtggag tcttccatcc
     7321 ggaaatgttt tcgtttccca acataatggt gctatttctc gccgttatgc ttacggatgt
     7381 tattcttctc gatgttttta actcgctggg cctgcccacc tctaccacgg tttcgttggt
     7441 attcgaactg ctgggttcag ccgtggcggt agcgatcttc agcatctggc aggcaaatgc
     7501 gggcgattta attgaataca tcaacaccgg caaggcgttg gccattatct ccggtatttt
     7561 tgtctctgta gccgttgctt tcatcgtggg tagctcggtg atgtatatta cccggattat
     7621 tttctctttt aaatacaaaa gaacatttaa atttttcgga gcgctttggg gtggatttgc
     7681 cttaactgcg atgacctact tcatcgtatt caagggacta aagaacagtc cgattgtggc
     7741 caccgatttc cttaatttag tcgaatccaa catgctgaag tccctgttgg ttacttttgc
     7801 tggatggtcg gtatttatgg cattgttgca gttcttattc agggttaaca tacttaaaat
     7861 aatcgtacta acaggaacca tggcgttagc gcttgctttc gccagcaacg acctggtaaa
     7921 cttcatcggt gtacctcttg ccggattgga tgcattcaaa aatgcaagtg ccacgttagc
     7981 ttcaggtagt tcgatagata cgttatacat ggatgctctg aacggtcctg tgaaagcaga
     8041 cattagaatg cttctcgcag cgggcatagt gatggttctg gccttgtggt tctccaaaaa
     8101 agctaaaaca gtaacggaga ccgaagtgaa tctggcacgt cagggtagcg gactggaaag
     8161 gttttcgtct actcctttat caagatcgat tgtccgttgg ggcgtggaca ctaatcgctt
     8221 tctcaataaa ctgacgcctt cttctttcag ggaaaaactc aacaatcgct ttgttattcc
     8281 cgaacaaata gaagaacagg gtgtctcttt cgacctgatc cgcgcttctg taaatttaac
     8341 cattgcagcc ctgctgatct ctctcggcac ctccctgaag ttgcccctgt ctaccactta
     8401 cgtgactttt atggtagcta tgggtacttc gctcgcagac agggcttggg gacgtgaaag
     8461 tgcggtgtac aggattacag gtgtactaac cgtgattggc ggatggttta tcacagccat
     8521 cgtagcattt accgttgcta tggtggtggc catgttccta gcctggggcg ggaaaattgc
     8581 catctttatc gcattggccc tggttggttt tatattgttc aaatcttcca agtttcacca
     8641 aaaaaggaaa ctggctgaaa aagcaaacgc tacattgatt gaggatgaga gcggcatcgt
     8701 gaaaacttca acgaccgaag taagggaggt catcgggaaa atgctggaca tttatcacca
     8761 gaacgtgaag ggactaacca aggaaagccg ctccttgttg agggagattg cttccaaggc
     8821 ggatgagtta tacctgaact acaaaaataa aaggacatac gaggttgtac caactatcca
     8881 gtccattaca ttgaaagagt tggacataga acaggaatat gttcagattg tggactacac
     8941 atacgaaata accaaggcat taagagtaat tacttccgac agttcactgt acatcgagaa
     9001 caaccacaaa ggatttaacg atgagcagga agaggatctg gaagacttga gtaaaaaagt
     9061 gaccaacatg tacaaaacgt ttattgatat gatggagaga aacgattact ccggttttgc
     9121 tacgataatc aatatcaggg acgagataat agaacaatgc gccaagctta ccaaaaaaca
     9181 gatacgaagg gtaaaagaca aagaatcagg tacaagaaac agtattttgt ttatgaacat
     9241 cctcaatgag accaagacca tcgtccttca atcggttaac ctgatgaaat ctcaacgaaa
     9301 catgatactg accgtaaaaa aaatatctga agaggtaacc gaaaagaaga actagccctc
     9361 cccgcgttgc cgcatggctt caaacgtgag cacggcagtg gagaccgaga cattcagcga
     9421 atcaattttt ccccgcatcg ggattttgat tcgctttgtt gcatttttga gccagaagcc
     9481 ggtcaggcct tcagcttccg tgcccatcac aatcgcagac ggcatacgga aatcaatgtc
     9541 ctggtagaac tcggcagcct gcaattcggc ggcataggat gagatgttgt tggctttcag
     9601 ccacgctgcc gcatcggctg atgagcaaac ggccgtttgc accgtaaaaa ttccgccaac
     9661 actggaacga accacattgg gattgtataa gtcggtctgc gggtcacaca caaccacagc
     9721 atccacggct gcagcatcgg cagtacgcag aatcgccccc agatttccag gtttctccac
     9781 tgcctccaaa acaattacaa acggatttcc agagagtttt atgtattcaa gtgtatgtag
     9841 cttgggtttt gcaatcgcca caacgccgtc agagttttcc cggtaggcaa tcttaccaaa
     9901 aacctccggt gaaatatcca aaacattttt ctcctccatg ctgtctaaaa caccgggata
     9961 cttggttttt tcaaacattt cccaacaaac gtataccgat tcaacgatat aatcgctttg
    10021 taaagccagt gacaactccc gggcaccctc aattacaaaa agctgctgct ctttgcgttc
    10081 cctgcttttt gcaagtttta caatgttttt taccgacgga ttttgaaggc ttgtaataag
    10141 catataactt tattattttc cctcttcctt gttcagaaga agagtatcga cagctttttt
    10201 aagctggctc ttgggtgctg cacccaatac cccttgcggt gttccggtca taggaacata
    10261 aagtattgtg gggatactct gaataccaaa tgcgcgggca agttcagttt cttcatctac
    10321 attcacctta taaacataga tctcctctcc atattcggcg gcaagttctt ctaaaacagg
    10381 agcaatttgc cggcaagggc cgcaccacgc ggcccagaaa tcgataatgg caggcttgtc
    10441 tcctttatac ttccattcct tttcgttgtt gtagtcaaaa accttttccg taaaactcgt
    10501 cgtgttcaac gcgatgggtt tcactgtttt tttaacctgt ggttctgctg cccatgcgga
    10561 aagaaccaat gcaaaaaccg ccactattac cgttaatttt ctcattttta ttgattgtat
    10621 atacctgtga tttataaaat gtttcctata aacgccttca tacggacaaa agtttatcga
    10681 aaacgtttaa tctgtccctg aagtttaaaa ctccattcga tctttatacg atatgcttcc
    10741 cgggcgcatg atcccgaatt ctttcgcgcc cacactatcc atccttaatt tccagatctt
    10801 cacgttattt accgccggga ctgaataggt aaacttttta tcgacatact gtcgcataat
    10861 gatatggagt gctttttctt tttcggcaaa atcatcctcg aattctacct caccttcgca
    10921 gataacactt cccgccttca tccggtaact acatgccaca tcgggatgct ggcggataag
    10981 ttcggggtcg gagcagaagg tgacacaaat ccgcggattt ttctccatag tacggatgga
    11041 acgaccctcc tgtgcagaat gcaggtagat aacgccatct tcataaccga aattcatcgg
    11101 caacacatac ggacgtcctt cttcgtctgc catacctaca aaacatacct tgcacgaacg
    11161 gataacggtt tcaatacgtt cccgctcttc tattgaaaat gttttcatgc tctttcacaa
    11221 ttctttctca gaagtagagg gcccgtctac actaaggaca cccttttcat ccatttttaa
    11281 ttcatcgata aaaacaacac gctcttctcc cgttttacgg gttcgtccat gataaacggt
    11341 atacatcttc tcactacctt tcaaacgaaa catcatgtta tgcccggttc cggttacatt
    11401 gccgccaccg tccctgtttt tttcaagtat cgggttattt ccggctttta caaacggacc
    11461 caggggattg tcggaaacgg catatcccac agcataattt tgcccaccaa aaaagttggc
    11521 agagtacatc atgtagattt ttccaccgtg ctcaaaagcg aacgagcctt ctgtccaacg
    11581 ccggtttacc tcaccattcg taacagaacg gctttcccat tccgtttgca ggtcactcat
    11641 tttagaaggt ggctgcaaaa gcaagaccgg ttcaccgatt acggatgtga agtcgttgga
    11701 tatctccacg ccatacaccc aactttcttc aatttcatca aacaggtttt gtgagcgtgc
    11761 ccattccgcc acttcacttt cgacggggtt tttgtaacag caacgtgaat agtaaaggta
    11821 tgtttttccc ttatgcttca agatattggc atcgataatg ggataaccgg gatcgaaaag
    11881 cggcttattg gacagctcct tgaacggccc tatcggttta tcggctactg ccaccccaat
    11941 acggaaattc tccgactcat ttgtgggatt taccttccag tcagcgctga aaaacatgta
    12001 gtatttgccg tctatttcat acacctcagg cgcccaataa tttttcaaag tccacgattc
    12061 tggagtgtct cccgaataaa cttgtccttc gtactgccaa tccgccaaat tgttagaact
    12121 gtacgcgcca aaaccttttt ccaccccacc cgttccgtac atataatatt ttccgtcgga
    12181 agccgaaaga ataaatggat cgccaagagc aaccggcaag ggattagtat acctgactgg
    12241 ccctgatgat ttattgcccg gtgcaataca tccgggaaaa ataagaagaa ataaaatgac
    12301 cgtcagcaaa ttttgaagaa tcgttttaac ctgaaattgt ttatgcatat ttttcgacat
    12361 ccattctttt ttttaattcg acagctttct ctcccgacac gaacaccgag ggtaatttag
    12421 gatgtagctg gagtttgtag ccggcggcgt tactctcaac ccaggtaatt ttatttttat
    12481 ttataataaa ctcatcacgg caccggaaaa gttgctcgaa atgcgtattt tctgcttcaa
    12541 actcctccaa cgtagcattt attgttagct cttccacgct tccgttattg gaaacattga
    12601 ccactagata atcccctttt gattctgccg aaataaacgt attgatatca ataggtttaa
    12661 taaatcgttg caggttgata aattgaggat ctgcctcagc aggcgtttct tttttatcca
    12721 gcattaaact gatgttcatt gcagtcgcct tccgcaagcg ttttttcata aaataaatat
    12781 gccggattaa ttgcgctgtc agacacggaa agaaacccac cgataccact acccacaaca
    12841 tccccagata accgtgtata tttggcggga aaacacctct gatataattg atcaaccaaa
    12901 ttgttgaggc aatggtcatc atctgataaa cacacatgaa tagttcctta cctactatcc
    12961 atctttcgtt tgaaaaaaaa cgcgggaaga atttgagcca gacaaaattg atcagcatag
    13021 tgacgtaaga acctcctgca tataacgctg ccgtgagaaa cggattatcc agaatgttcc
    13081 tgtctcctaa attaaaaggc tgtaaaaaac tcaatactaa aaaaacacct gcagcaatca
    13141 cagtacaggt agcgatgtaa actttaaaat tcgcatatcc cgggtgagga cggcaaaata
    13201 caactaagat ccgactcata gaaacagaaa aaatcaatat tcaagttgta aatataatga
    13261 aaaaaaacag ctctctccct tttcaccttg aaattttatt cctcactgaa gaaatctgcc
    13321 cgatcttctt aagacaggcg acaaataata ataaaagggg aaaaaccggc cggcctttcc
    13381 ccttttgtta tgttatctca gagaggtttt atttcacctt atccacgatg gccttgaacg
    13441 cttcgggatg attcatcgcc aaatcggcga gcactttacg gttgatatct atttcatttt
    13501 tgtgcaatgc tcccatcaga cgggaatagg acatgccata ctgacgtgcg gcggcattga
    13561 tacgttgaat ccaaagtgcg cggaagttac gctttttatt cttacggtca cgataggcat
    13621 aggtcaaccc cttttcccac gtgttcttgg ctacggtcca cacatttttg cgtgcgccaa
    13681 tataccctct tgttaatttc agaacttttt ttctgcggtt gcgtgatgca acatgattga
    13741 ctgatcttgg cataatgtta cttgtttttg aatgtcagcg ttcccccggg agggaatctt
    13801 tggtgcatac atccggttaa taaatcgtta agttggaaag cttcgggctt atttcaatcc
    13861 caataagtgt tttacgctgt tctcatctac tttgtgaaca agtcctgtct gcgtaagatt
    13921 tctcttttgc tttttggtct ttttcgttaa gatgtgactt ttgtacgcat gctttctctt
    13981 gatttttcct gttccggtaa gggcgaacct ctttttggca ccggaattag tcttcatttt
    14041 tggcattttg ctcttgtttt ttgtaaatta tttagtttag ataatcaacc cgatattgct
    14101 cttccccaag gggtctcgca atatcgccgg ttgatgttta tgttgaggct tcctcagcct
    14161 ttttcatcaa aaagtgtgca aagatacaac atgtttttga aaaatcgatg cgacttttct
    14221 ttttttatac ttttattccg aaggttgttc ttttttagca gatccctgct tcttgggtgt
    14281 cagcattatt atcatgcgtt ttccttcgag caccggtagc tgttctacac gggcatagtc
    14341 ctccagatcg ttagcaaaac gaagcagcaa cacttctccc tgctccttga acagaatgga
    14401 gcgtccttta aaaaatacgt aagccttcac tttagctcct tcttcgagga aacttttggc
    14461 gtgtttcagt ttaaagttgt aatcgtgatc atcggtctgt gggccgaaac gaatctcctt
    14521 gaccacgatt ttaaccgctt ttgctttttg ctctttttgt tttttctttt gctggtacag
    14581 aaatttctga taatcagtta tccggcaaac aggcggaacg gcagttggcg aaatttccac
    14641 aagatccaat tctttctctt cggcaatccg caaggcttct ctggtggggt atatcccctg
    14701 ttctacatta tcgcctacaa ggcggacttc gggcacacgg atacgttcgt tgatccgatg
    14761 ttgatctttt gtgtctctaa agttattcct cattcggata gtattattcc tttctttata
    14821 aatgttagta ctttttagat tgtcgaaaat gaattaaata tttacctgtg gattcatcat
    14881 ttcatccacc tcctgtttta ttctgtgtgc aaaagtagtt aatttttctg aaccttgatc
    14941 aattccgcct tgttttctta ccgaaacttc ttcattttct gcctctttct cacccacaat
    15001 caatagatat ggggtgcgtc taagctcgtt gtcccggatt ttcctgccca ctttttcatt
    15061 ccggtcatcc acttctgcac ggatatcctg catctttaat gttcgagcga cagaataggc
    15121 gtaatcgtta aatttctcgc tcacaggaag gattaccact tgagtcggcg tcaaccacag
    15181 tgggaacttt ccggcagtat gctcaataag tacagccaca aaacgctcca tggaaccaaa
    15241 cggcgcacgg tgaatcatta ccgggcgatg tttctggtta tccgctccgg tatattccag
    15301 ttcaaacctg tctggcaggt tataatccac ctgaatggtt cctaattgcc agcgccggcc
    15361 aagggcatct ttcaccataa aatcgagttt tggcccatag aaggcagcct ctccgagctc
    15421 cacccgcgct ttcattcctt tttcttcaca agcttccagg atagcgcgtt ccgctttatc
    15481 ccaattctcg tctgtgccaa tatatttttc tttgttttcg ggatcccgca aagagatctg
    15541 agcttcaaaa tccttgaaat ccaacgcctt gaagataatg aaaataatgt ccatcacttt
    15601 caggaattcg tcttttacct gttcgggcat acagaaaatg tgcgcgtcat cctgtgtaaa
    15661 gcctcgaacc cgtgtaagcc cgtgtaattc accgctttgc tcgtagcgat acaccgtgcc
    15721 gaattcagca aggcgaagcg gtaaatcttt gtaagaacgc gggaaagcct tgtatatctc
    15781 acagtgatgc ggacagttca tcggttttag cagaaactcc tcatcttctt cgggggtacg
    15841 aatgggttga aacgaatcct taccgtattt ggcatagtgc cccgatgtca cgtagagctg
    15901 ttttcccccg atatgaggcg tgataacctg ttcgtattcg aattgttgtt gaattttttt
    15961 tagaaattct tccagtttca accggagttg agttcctttg ggaagccata acggcagtcc
    16021 agctccgaca acttgagaga aggtgaaaag ttcaagttct tttccg
//
LOCUS       DAOSDR010000013        15942 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrimonas sp. isolate AMR_MDS_1826
            NODE_1486_length_15942_cov_3.926292, whole genome shotgun sequence.
ACCESSION   DAOSDR010000013 DAOSDR010000000
VERSION     DAOSDR010000013.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863355
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrimonas sp. (wastewater metagenome)
  ORGANISM  Petrimonas sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Dysgonomonadaceae; Petrimonas.
REFERENCE   1  (bases 1 to 15942)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15942)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:10:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,720
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 35
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15942
                     /organism="Petrimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1486_length_15942_cov_3.926292"
                     /isolate="AMR_MDS_1826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2023866"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(40..975)
                     /gene="queG"
                     /locus_tag="PLI30_01080"
     CDS             complement(40..975)
                     /gene="queG"
                     /locus_tag="PLI30_01080"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006800531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HOI78178.1"
                     /translation="MTFSEEIKQHALSLGFDACGICRAEESEQEAHYRSWLSEECHAG
                     MDYLERNIDKRMDPRLMVEGARSIISVALNYFPHRFQHEDAPQFAYYAYGEDYHDIVK
                     EKLSRLFDFIKLRFPGVSGRYFSDSAPVLERFWAARSGLGFVGKNTLLIIPGKGSYFF
                     LGELIVDLELDYDSPISQNCGKCRRCLDACPTGAIEKPHWVNARKCISYQTIENKGEI
                     SSEIVPRLSNNVYGCDICQVVCPWNRYARPHSTPEFNPSDEFLSLDYKRLQEMDEDTY
                     RRIFRRSAVKRAKFAGLKRNLDAWKSSQQTEGGIS"
     gene            1092..2564
                     /locus_tag="PLI30_01085"
     CDS             1092..2564
                     /locus_tag="PLI30_01085"
                     /inference="COORDINATES: protein motif:HMM:NF024832.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HOI78179.1"
                     /translation="MHKLNPDMADSALKKKTTVSLFWSFLDKFGQQMLNFASMLILMN
                     IVAAEDYGLVGSLAVFVAFSTILIDSGFGRALLNRKDLTQTDYTTVFYFNISLSFVLY
                     LLFFFTAPLLGKMFHAPAITSVVRILFLSLIFNAFGLIHQTILTKKANFKGLTQVNTL
                     SLLFSDVVAVVMAIAGYGVWALVAQTLLYAFFRSLFLWVYSPWRPIARFSRKSLGTFF
                     AFSNKLLSASVISTTVNNIYPSLIAAFYPMSQVAYFNQAKKYQDIPFLTLANTFRTVA
                     MLILSEINEQTERLKRVVSKIIKSIAFLSFPIGLTMIVIAEPTFHLLFKEKWLAAVPY
                     FQILTFAGMLSPFIFIFQELFIAKENSKFFLGIEVAKGVLLILLIVLLFPHGITALAV
                     SWIIYMVISLIISVMLTGKLIRYTLFHLIKDIGPYLLLAIISSAVSFLLTMKIGNNVV
                     FIILNLVITGTSYILLCKLFKLEMLKEIEYWFEKRKKEKK"
     gene            2561..3268
                     /locus_tag="PLI30_01090"
     CDS             2561..3268
                     /locus_tag="PLI30_01090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI78180.1"
                     /translation="MKEFLLYVRFAIDYALNNKTLSHIKQFSRWYKDKKSGDTTLNRR
                     SPWMTYDAVDFLSATCRPGMSVFEWGSGGSTLFFASRCKQVHTVEHDPDWKDFLNKKI
                     EELDLKNVSIQEIAGKPIADFNERDYRNPDDFVSKDKKSAGLSFEDYVKAIDTFPEEY
                     FDLVVVDGRVRNCCVKRAIPHVKKGGYLIVDNTDRSYYLAPFPELQDPSKWKKTEFQG
                     PVFFQHAFSKTSFFKKA"
     gene            complement(3270..5414)
                     /gene="rnr"
                     /locus_tag="PLI30_01095"
     CDS             complement(3270..5414)
                     /gene="rnr"
                     /locus_tag="PLI30_01095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019537971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008997 - ribonuclease R activity
                     [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HOI78181.1"
                     /translation="MSKRTSVAETVSKPKKKQLEQLVVDFLTENKDKQFNYKQIAAAL
                     NIRGEEGRRVLVKALDRLRDYDVLLETSRGRYRLNNRGLILEGRFERRSNGKNFFVPD
                     DDANIIYIPERNSKHAMNGDRVRVQLLAKRKRADTEGAVVEILERAQTRFVGVLEVQK
                     YFAFLVMDSKFLANDIFIPTDELNGAGNGDKVLVEIVEWPEKANNPVGKVIDVLGKPG
                     DNTTEMHAILAQYGLPYRYPGDVEKAAELIPEKIDEKEIALREDFRDVFTITIDPKDA
                     KDFDDALSLRKISPTLWEVGVHIADVTHYVRQGDVIDKEAENRATSIYLVDRTIPMLP
                     ERLSNGLCSLRPKEDKLCFSVIFELNEYAEIKKSRIVRTVINSDSRLTYEEAQTVIET
                     GKGDFSSEILVMNSLAQKLREKRFANGAINFERYEVKFNLDEKGKPLGVYFKESKEAN
                     HLIEEFMLLANRTVAEFIGKVPKGKHAKAFVYRIHDIPDSEKLDTLNAFILRFGHKIK
                     TEGNKVEVAKSINSLLDKVQGRPEENLVETIAIRTMSKAVYSTKNVGHYGLAFDYYTH
                     FTSPIRRYPDMMVHRLLERYLDGGRSVDQTELEMECKHSSDMEQVAANAERDSIKYKQ
                     VEFMADKIGKVYDGVVSGITEWGIYVEINENKCEGMIPIRELDDDFYELDEKNYRLVG
                     RRTKREYRLGQPVSIQVAKANLERKQLDFVLA"
     gene            5653..6666
                     /locus_tag="PLI30_01100"
     CDS             5653..6666
                     /locus_tag="PLI30_01100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010182595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HOI78182.1"
                     /translation="MEQKKILITGASGFIGSAAIDKALELGYETWAGIRASSNKVYLQ
                     DERIKFIDLNYGDKKKLKEQLQDFTDKNGRFDYIVHSAGITKALHKAEFDRVNYEQVR
                     NFVDALVETDAVPDLFVFMSTLGAVGAGDEIKYTPLSCDCTPNPNTAYGKSKLKAENY
                     LKKLPGFPYVILRPTGVYGPRDKDYLILMRAVKNGINVGAGFRKQILTFIYVGDLVRV
                     IFNCIEKNTHRKEYFVADGDVYTDSEFNTIVQQALQKKRVFRVNVPLWLLKPAAFISE
                     RISGLLGKATAFNTDKYKIMKQRNWSCDTTPLQHDLDFRPGYRLKEGVEATVKWYRER
                     GWL"
     gene            6781..7503
                     /gene="dapB"
                     /locus_tag="PLI30_01105"
     CDS             6781..7503
                     /gene="dapB"
                     /locus_tag="PLI30_01105"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014224698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HOI78183.1"
                     /translation="MKIALIGYGKMGHEIEKIALQRGHEIVCTIDLNETGKFDSPEFR
                     SAEAAIEFTSPESALQNYRRAFAANVPVVSGTTGWLEHLNEIKAACENEGQTFFYASN
                     FSLGVNIFYALSNYLAKVMSQFSDYDVRMEETHHIHKLDAPSGTAITLAEGILENLGR
                     KETWSLNKAGNENELEIKSFREGEVPGIHTVVYESEADSIRITHDAKSRKGFALGAVL
                     AAEFTKGKKGFLGMKDMLNLHP"
     gene            7663..9081
                     /locus_tag="PLI30_01110"
     CDS             7663..9081
                     /locus_tag="PLI30_01110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019537980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S26 family signal peptidase"
                     /protein_id="HOI78184.1"
                     /translation="MWFGVWILSTILFAVWAGSPWILLFILLFFDVYITKFVPWSWWK
                     KTENKVVRKTMEWVDAILFALIAVYFINTFFFQNYQIPTSSLEKSLLVGDFLAVSKVS
                     YGPRAPITPLSFPLAQHTMPVIGGKSYIDKPQWKYRRLKGLGEVKRNDIVVFNFPAGD
                     TVALNRQAEDFYLLSKYNANGSAGIRSDQRTYGEVVFRPVDRRENYVKRCIGLPGETI
                     ELRDDSVYIDGELIPSPKLSQLTYMIHTDGTVISEQIFQELGINKDDYMMPNSYGQVS
                     LKMQDLTGIDSLTMAHSGLKQQNGNNGKIYYNIPLTGAMAAKLKEKPFVWEVVKDVEQ
                     PGSSYYYPLGYTTNWTRADFGPLWIPEKGATLTFDTDVAFKAAAYERCIKNYEGNDFA
                     FRDGKVYINGEPADSYTFKMDYYFMMGDNRHNSADSRVWGFVPEDHIVGQPMLIWLSL
                     EKDNGWLNGKIRWNRLFRSAKK"
     gene            9048..9686
                     /locus_tag="PLI30_01115"
     CDS             9048..9686
                     /locus_tag="PLI30_01115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019537981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WbqC family protein"
                     /protein_id="HOI78185.1"
                     /translation="MEQVISKCKKITGRVLLSSFYLAPVEYYSVFFRASSTVIEVHEN
                     YQKQSYRNRCNIVGANGSMALSIPVEKPSAVKCRMKDVRIADHGNWRHLHWNAIVSAY
                     SSTPFFEYYADELQPFYEKRIPFLVDFNLQLHELICGWLRIEQPTNLSPEYVAEIPEG
                     IADHREAIHPKRPSGFMTRPYYQVFQDKLGFIQNASIIDLVFNMGNEARLWL"
     gene            9737..11269
                     /gene="gltX"
                     /locus_tag="PLI30_01120"
     CDS             9737..11269
                     /gene="gltX"
                     /locus_tag="PLI30_01120"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019537989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HOI78186.1"
                     /translation="MMSRKTRVRFAPSPTGPLHIGGVRTALYNYLFSKQQNGDFILRI
                     EDTDSTRFVPGAEAYIQEAFDWLGLPYDESPAKGGKFGPYRQSERKDIYKKYVHKLLD
                     EGKAYIAFDTPQELEAKRNEIPNFQYDATTRLQMRNSLTLPTEEVKKLIDNGTQYVVR
                     IKIEPRIDITVNDLIRGEVVINSSVLDDKVIYKSADELPTYHLANVVDDHLMEITHVI
                     RGEEWLPSAPLHVWLYRSFGWEDTMPEFAHLPLLLKPEGNGKLSKRDGDRLGFPVFPL
                     EWKDRQSGETSSGYRESGYLPQAVINFLALLGWNPGTEQEIFSLDELVTAFSFERCSK
                     SGAKFDFEKAKWFNHKYIQGTPNELLAEMIQPVLAEKNIPCDKNYAANVIGLVKERVN
                     FVKELWDQASFFFIAPASYDEKTVKKRWKKESSAQLSELINVLQSIDDFSSENQEIRV
                     KQWIEEKNYPIGNIMNAFRLAVVGESKGPHMFDITSLLGKQETIGRLTTAITNIPAPE
                     NT"
     gene            11306..13387
                     /locus_tag="PLI30_01125"
     CDS             11306..13387
                     /locus_tag="PLI30_01125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019538002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HOI78187.1"
                     /translation="MNLKSKRKFRLRTRFFVPAVFILAIALVTLLFPRQGDFKYSFSE
                     GRPWQYGLLTAPFDFPIYKPADQLKAERDSILRFYEPYYTIDESVEKNAMAEFDADVN
                     LNTKLSSLSPDYILYLRNSLQKIYRSGIMRSEDYDKVFSSETQSMRLRKGNLAESKSV
                     ETFSTIKSAYEQLLNNTPKSMDAELIRLADVNKYIRENIVYDASTSEKAREEFIQQVS
                     PSTGMVQTGQRIIDQGEIVSSQTYKVLNSLKRVTEERSGRTGKNGWMIFGQLLLVVLL
                     FGAFYAYLLFFRPHEYRNRKHVTFMVLLVTSFVALTAITSQLDLFNVYIIPYAIVTIL
                     IRTFIDSRTALFASLITIILSSLMVPFPFEFIVIQIAVAMVSVFMLKELSERYQLIRS
                     SFFILIAYSLMYIGLVMHQEGNINKIDPIIFIYFFINFIFILFSYSLVYLIEKSFGFI
                     SGVSLIELSNINKPLLKELSEKAPGTFQHSLQVSNLGMAAAVKIGANASLIRTGALYH
                     DIGKMVNPAFFTENQSPGMNPHAGLPFEESARIIINHVRDGVKIAQKNNLPKQITDFI
                     ETHHGTSMPKYFYISWKNANPGKEINEDLFRYPGPNPFSKETAIMMMADAVEAASRSL
                     PEYNEDSIRNLVESLIDSQVTEGYFKLAPITFRDIAAIKEVFLQKLQTIYHTRIAYPK
                     LDKPQTQPESV"
     gene            complement(13394..14914)
                     /gene="guaA"
                     /locus_tag="PLI30_01130"
     CDS             complement(13394..14914)
                     /gene="guaA"
                     /locus_tag="PLI30_01130"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015532441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HOI78188.1"
                     /translation="MHEKIIILDFGSQTTQLIGRRVRELNTYCEIVPFNKFPFGDRSV
                     KGVILSGSPFSVNDDNAFKTDLHEIRRNYPVLGICYGAQWIAQSSGGTVDKSDSREYG
                     RAHLSVKETGDLLLGNLPQQSQVWMSHGDTIVRIPDNFSVIASTEEVEIAAYKVKDEP
                     TWGVQFHPEVFHTEQGNKLLDNFLAICGCRKDWTPASFIEATVADLKETLGDDKVILA
                     LSGGVDSSVVAVLLNKAIGKNLTCIFVNHGLLRKNEFETVLENYEHLGLNVIGVDARE
                     RFYKELAGVTDPERKRKIIGKGFIDVFDEEAHKLTAIKWLAQGTIYPDIIESLSITGT
                     VIKSHHNVGGLPEKMNLKLCEPLKLLFKDEVRKIGLELGMKPHLIYRHPFPGPGLGIR
                     ILGDITPEKVRIAQDADDIFVSNLRAAGLYDKVWQAGAILLPVQSVGVMGDERTYENT
                     IALRAVTSTDAMTADWAHLPYEFLAKVSSEIINKVKGVNRVVYDISSKPPATIEWE"
     gene            15196..>15942
                     /locus_tag="PLI30_01135"
     CDS             15196..>15942
                     /locus_tag="PLI30_01135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOI78189.1"
                     /translation="MGQSVDDAKKWYLEGDFTQAKPVFEAEYRVNPNNAQLNLWLGVI
                     SLAESDYQKAQQYLEFASLKNIQESYLQLGQLYTKMYRFADAEKEFTRYEKANRRNKQ
                     ALALLNPYKEYADKLKRLINRTEDVQIIDSIVVPKSDFLSVYKLSASAGSLQPVSGFF
                     RDQPNNNQVLFLNERQDKIYYPQDDSISGSNLYTMEKLLDSFGNEKRLSESINQEGNQ
                     AYPCVMPDGLTIYFSSTGHVSLGGYEIGRAH"
BASE COUNT         4390 a         3819 c         3559 g         4174 t
ORIGIN      
        1 gcatagttga atgtgcaaaa gtagctaaaa atagtgattt taggaaattc caccctccgt
       61 ttgttgggaa cttttccagg cgtcgaggtt tctttttaat ccggcaaatt tcgctctttt
      121 taccgccgat ctccggaaaa tccgccggta ggtatcttca tccatctcct gcagcctttt
      181 gtaatcgagc gaaaggaatt catccgaggg attaaattcc ggcgtactgt gcggccgggc
      241 ataccggttc caaggacaga caacctgaca gatatcacaa ccgtatacat tattactcaa
      301 acgcggaaca atctcggacg atatttcacc cttattttca atggtttggt acgatataca
      361 cttccgggcg ttcacccagt gcggtttttc aatggctccg gtgggacagg cgtccaggca
      421 acgacggcac ttcccgcaat tttgtgatat gggtgaatcg taatccagtt caagatcaac
      481 aataagctct cccaaaaaaa aataagaccc ttttccggga atgatcagca aggtgttttt
      541 gcccacaaat cccaatccgg aacgtgccgc ccagaaacgt tccaatacag gagcagagtc
      601 tgagaaataa cgccccgaca ccccgggaaa acgaagcttt atgaaatcga aaagccggct
      661 gagcttctct ttgactatat cgtgataatc ctcaccgtaa gcgtaatacg caaactgggg
      721 agcatcttca tgctgaaaac ggtgggggaa gtaattcaac gcaaccgaga taattgatct
      781 ggctccttca accatcagcc ggggatccat tcgcttatca atattccgct caaggtaatc
      841 cattccggca tgacattctt ctgagagcca gctcctgtaa tgggcttcct gctcactctc
      901 ttcggcgcgg caaataccgc aggcatcaaa acccagcgag agggcatgct gttttatttc
      961 ttcggaaaaa gtcatttaaa gataagaacc atgagccagg aacagtttct aaaaatcaaa
     1021 gataagaaaa ctatagtatt aatgaataag cacatcaaca aaaagtcttt tgtctgaaaa
     1081 aaaccttact tttgcataaa cttaatcccg atatggcaga ttcagccctg aagaaaaaaa
     1141 ctaccgtctc tcttttttgg agctttctcg ataagtttgg gcagcagatg ctcaactttg
     1201 ccagtatgct gatactgatg aatatcgttg ccgctgaaga ttacggcttg gttggctcct
     1261 tggcagtctt tgtggcattc tctaccatcc tgatcgacag cggattcggg cgagcacttc
     1321 tgaaccggaa agacctgacg caaacggatt acaccaccgt tttttatttc aacatttcac
     1381 tgagctttgt cctttatttg ctcttcttct ttaccgcccc tctgctggga aagatgttcc
     1441 atgcccccgc gatcacaagc gttgtccgga tcctgttctt gtcgctcatt tttaacgcat
     1501 ttggcttaat ccaccaaacc attctcacga aaaaagctaa tttcaagggg ctcacccagg
     1561 taaatacact ctcactcctt ttttccgatg tagtggccgt tgtaatggcc attgccgggt
     1621 acggtgtatg ggcactggta gcccagacac ttctctatgc ttttttccgc tccctttttt
     1681 tgtgggtcta ctccccctgg cggcctatcg cccgtttcag cagaaagagc ttgggcacat
     1741 tttttgcttt cagcaacaaa ttgctgtccg catcggttat ttcaactacg gtaaacaaca
     1801 tttacccgag tttgatcgca gctttctacc cgatgagtca ggtggcttac ttcaatcagg
     1861 caaaaaagta ccaggacatt ccgtttctta cactggcaaa cacctttcgt accgtagcaa
     1921 tgcttatttt atcggagata aacgaacaaa cggaacggtt aaagcgagtg gtcagtaaga
     1981 tcataaaatc catagcattc ctatcattcc ccatcggact taccatgatt gtgattgcag
     2041 aaccaacgtt tcacctcctt ttcaaagaga aatggctggc agctgttcct tattttcaaa
     2101 ttcttacgtt cgcaggaatg ctttctcctt ttatttttat ctttcaagaa ttgtttatag
     2161 ctaaggagaa ctccaagttc tttctgggca ttgaagtggc aaaaggggtt ttgctgattt
     2221 tactgatcgt tcttcttttt ccgcatggaa tcaccgcttt agccgttagc tggataatct
     2281 acatggttat ttcactgata atttccgtaa tgttgacggg aaaacttatc cgatacaccc
     2341 tttttcacct gattaaagat atagggcctt atttactcct ggcaattatc agttctgccg
     2401 tgtccttcct tcttacaatg aaaataggga acaacgttgt ttttataatc ctcaacctgg
     2461 ttatcacggg cacgtcatac attcttctct gtaagctatt taaactggag atgctgaaag
     2521 agattgagta ttggttcgaa aaaagaaaaa aagaaaaaaa atgaaagaat ttttgttata
     2581 tgttcgcttc gcgattgatt atgcattaaa caacaagaca ttatcgcaca taaaacagtt
     2641 cagccgctgg tataaagata aaaaatcagg tgacactacc ttgaaccgcc ggtcaccctg
     2701 gatgacctac gatgccgttg attttctttc ggcgacatgc cggcccggca tgagcgtttt
     2761 tgaatggggg tcgggcgggt ccaccctgtt ttttgcttca cgctgcaaac aggtgcacac
     2821 cgttgagcac gatccggact ggaaagattt tttgaataag aaaatcgaag aactggattt
     2881 aaaaaacgta agtatccagg aaatcgcggg aaagcctatt gctgacttca acgaaaggga
     2941 ctaccggaac cccgacgatt ttgtatcgaa agataaaaaa tcagcaggac tctccttcga
     3001 agactacgta aaagcaattg atacttttcc ggaagaatat ttcgatttgg tcgttgtgga
     3061 cggaagggtt agaaactgtt gtgtgaaaag agcaattcct catgtgaaaa aaggaggtta
     3121 cctgattgtg gacaataccg acaggagcta ttaccttgcc ccttttcccg agcttcagga
     3181 tccttccaaa tggaaaaaga ccgaatttca aggtccggtc tttttccagc acgcattcag
     3241 caagacttcg ttcttcaaaa aggcctgaac taggccagta caaaatccag ttgtttcctt
     3301 tccaggttgg cttttgcaac ctgtatggaa accggttgtc cgaggcggta ttcacgttta
     3361 gtgcgacggc cgaccaggcg gtaattcttc tcgtccagtt cgtaaaaatc atcgtccagt
     3421 tcacggatag ggatcatacc ctcgcatttg ttttcgttaa tttccacgta aataccccat
     3481 tccgtaatcc cggaaacaac accgtcgtac actttcccga ttttgtcggc cataaattcc
     3541 acttgcttgt acttgatgga gtcgcgctcg gcattggctg ccacctgttc catatcggaa
     3601 gagtgcttgc attccatctc cagctctgtt tgatcaacgg atcggccacc gtccaggtaa
     3661 cgttcaagca accggtggac catcatgtcg ggataacgcc ggatgggaga tgtgaagtgt
     3721 gtgtaatagt caaacgcaag cccgtaatgc cccacgtttt tggtagaata aaccgctttg
     3781 gacatggtgc ggatggcaat ggtctccacc aggttttctt cgggacggcc ctggacctta
     3841 tccaacaatg agttgatgct ctttgccacc tctactttgt tcccttcggt ttttatttta
     3901 tggccgaaac gaaggataaa ggcattcagg gtgtccagtt tttcggaatc aggtatatcg
     3961 tgaatgcgat aaacaaatgc cttagcatgt ttacctttcg gaacctttcc gataaattcg
     4021 gccaccgtcc tgttggccag aagcatgaat tcttcgatga ggtggtttgc ttccttcgat
     4081 tctttaaaat aaactccaag cggcttgcct ttctcatcga ggttgaattt cacttcgtag
     4141 cgctcaaagt tgatggctcc gtttgcaaaa cgcttttcac gtaatttctg tgccaggctg
     4201 ttcatgacca ggatctcgga tgaaaaatca cctttcccgg tttctatcac ggtttgtgcc
     4261 tcctcatacg tgagccggct gtcggaattt atgacggtgc ggacaatgcg tgatttttta
     4321 atctccgcat actcattaag ttcaaaaata accgaaaaac aaagtttgtc ttccttcgga
     4381 cgaagggaac acaacccatt gctcaacctt tcgggcagca tagggatagt cctgtcgacc
     4441 aggtagatgg atgttgccct gttttccgct tctttatcga taacgtcgcc ctgtctcacg
     4501 taatgggtta catcggcaat gtgcacaccc acttcccaca gtgtgggaga aattttacgt
     4561 agcgacaaag cgtcatcgaa atctttggcg tctttcgggt cgatggttat ggtaaacaca
     4621 tcgcggaaat cttcccgcag ggcaatctcc ttctcgtcga ttttttcagg aatcagttcc
     4681 gccgcttttt ccacatcccc gggatacctg tacggtaatc cgtattgtgc caggatagcg
     4741 tgcatctcgg tagtattgtc tcccggcttg cccaatacat caatcacctt cccaaccgga
     4801 ttattggctt tttcgggcca ttccacaatt tccaccagta ctttatctcc gttcccggcc
     4861 ccgttaagct cgtccgtcgg aataaatata tcgttggcta gaaacttgct gtccattacc
     4921 aggaaagcaa aatatttctg cacttccagt actcctacaa accgggtttg tgcacgctcc
     4981 agaatctcaa cgaccgcccc ttcggtgtcg gcccgtttcc tttttgccag cagttggaca
     5041 cgtacccgat ctccgttcat cgcatgtttg ctgttccgtt cgggaatata gataatgttg
     5101 gcatcgtcat cgggcacaaa gaagttttta ccgttgcttc ttctttcaaa gcgtccctcg
     5161 aggatcaatc cgcggttatt taacctgtat ctcccacgag aagtctccag caacacatcg
     5221 taatcgcgaa gccggtcaag cgcttttacc aatacacggc ggccttcttc tccccggata
     5281 tttaatgcag cagctatctg cttataatta aactgcttgt ctttattttc ggtcagaaaa
     5341 tcgacaacga gctgttccag ctgtttcttt ttcggttttg atacagtctc tgcaactgat
     5401 gttcttttac tcatatgatc tgtttcgttt acaatattaa acaacgaaat cctgttttag
     5461 ttagaaattg tcttgaattt ttaggtttag tttcataaaa actcaaaact gcttcttagt
     5521 acaaagtaac aaaaaatatt cgtttttcag atatttaact tcaacggaca ggacttttat
     5581 cggacatctt tagggaatat tgtttttaaa atgcgtggtt atgattaaat ttgtgattga
     5641 attttggaag gaatggaaca aaaaaaaatc cttatcaccg gagctagcgg ttttattggc
     5701 agcgcagcca ttgacaaagc attggagttg ggttacgaaa cctgggcagg aatccgtgca
     5761 agcagtaaca aagtttactt gcaggatgaa cggataaagt ttattgattt aaactacggt
     5821 gataagaaaa aactaaaaga gcagcttcag gatttcaccg ataaaaacgg acggttcgat
     5881 tacatcgtac acagcgccgg aattaccaag gctcttcaca aagcggaatt cgacagggta
     5941 aattacgaac aagtacggaa ctttgtagac gcacttgtcg aaaccgatgc cgtccccgat
     6001 ttattcgttt ttatgagcac actgggagcc gtaggtgcgg gcgacgaaat aaaatacacc
     6061 cctttgtcgt gcgactgtac tcctaatcca aatacggctt acggcaaaag taagctgaaa
     6121 gccgaaaatt acctgaaaaa gctacccggt ttcccgtatg ttattcttcg ccctacggga
     6181 gtatatgggc cgcgcgacaa agattacctg atcttgatgc gtgccgtaaa aaacggaata
     6241 aatgttggtg ccggattccg gaaacagatc ctcacattta tctatgtcgg tgacctggta
     6301 agggtcattt tcaactgtat tgaaaagaac actcaccgga aggaatattt cgtggccgat
     6361 ggggatgttt ataccgattc ggagttcaat accattgtac aacaagcgct gcagaaaaag
     6421 cgcgttttca gggttaacgt tcctctgtgg ttgctgaagc cggcagcatt catcagcgaa
     6481 agaatttccg gcctgctggg taaagcgact gccttcaata ccgacaaata caagatcatg
     6541 aagcaacgga actggtcgtg cgacaccact ccgctacaac acgacctcga cttccgaccc
     6601 ggctaccggt taaaagaggg ggttgaggcc accgtaaagt ggtaccggga aagaggatgg
     6661 ttgtaagctc gcggcttggt aaccgaaacg acatcgtacg tacaatttaa gaacaaataa
     6721 cgcaaaacag attcacaatc agttctattt ttgtattttt gcattttaac aattaaacag
     6781 atgaaaatag cattaatcgg ttacggcaag atggggcacg aaattgaaaa aatcgccctg
     6841 caacgcgggc acgaaatcgt ttgcaccatc gacctcaacg aaaccggtaa atttgattct
     6901 cccgagttca gaagtgccga ggccgccatt gagttcacct ctcccgaaag tgctctgcaa
     6961 aattaccgca gggcttttgc agcaaacgtt cccgtggtat ccggaaccac cggatggctg
     7021 gaacacctga acgaaataaa agcggcttgt gaaaacgagg ggcaaacctt cttttatgct
     7081 tccaacttca gcttgggtgt aaacatcttt tatgcactca gcaattacct ggcaaaggta
     7141 atgagccaat tttccgatta cgacgtgcgg atggaagaaa cacaccacat ccataagctg
     7201 gatgcaccca gcggaacagc catcaccttg gccgaaggta ttttggaaaa cctgggacgg
     7261 aaagaaacat ggtcactcaa caaagccggc aatgaaaatg aactggaaat caagtcattt
     7321 cgagaagggg aagttcccgg cattcatact gtcgtatacg aatcggaggc cgacagcata
     7381 cgaatcaccc acgatgccaa gagccggaag ggttttgccc tcggtgcagt cttagctgcc
     7441 gaattcacga aagggaaaaa aggttttctg ggcatgaagg acatgctgaa cctacatccc
     7501 tgattgcttc aaaagaagca taaacagcat caaccgattg aaacaattgc aaaaaacaca
     7561 aaaaagatga ataaaaccgc cgacaacaac aatgcctctt cccacaagtc tccttcggga
     7621 aatttgaagg tgcgcatttc ctctgcccaa cagcatcaat ggatgtggtt tggcgtatgg
     7681 atactttcga ccatcctctt cgccgtttgg gccgggagcc cgtggatcct gctcttcatc
     7741 ctgctttttt tcgacgtata catcacgaag tttgtaccgt ggtcgtggtg gaagaaaacc
     7801 gaaaacaagg tcgtccgcaa aaccatggaa tgggtggatg ccatcttgtt tgcgttgata
     7861 gctgtatatt ttatcaacac ttttttcttc cagaactacc agatccccac ttcatcactg
     7921 gaaaaatctt tgctggtcgg cgattttctg gcggtcagca aggtcagcta cgggccccgc
     7981 gcacccatca ctcccctgtc gtttccgctg gcgcaacaca ccatgccggt gatcggcgga
     8041 aagtcataca tcgataagcc gcagtggaag taccgcaggc taaaggggct cggtgaagta
     8101 aagcgtaacg atattgtcgt ctttaacttt cctgccgggg atacggtggc gctgaaccga
     8161 caagctgagg atttctattt gctgtcaaaa tacaatgcca acggaagcgc cggaatacgt
     8221 tccgaccaaa ggacctacgg cgaagtggtt tttcgcccgg tagaccggcg cgaaaattac
     8281 gtaaagcgct gtatcggact tcccggggaa accatcgaat tgcgcgacga cagtgtttac
     8341 atcgatgggg aactcatccc gagccctaaa ttatcgcagt tgacctacat gatccatacg
     8401 gacggaacag ttatatcgga acaaattttc caggaactgg gtatcaacaa ggacgactac
     8461 atgatgccga acagctacgg gcaggtctcc ttgaagatgc aggatttaac gggtatagac
     8521 agcctgacca tggcccattc cggattgaaa caacaaaacg gaaacaacgg aaagatctat
     8581 tacaatatcc cgttaaccgg agcgatggcc gctaagctga aggaaaaacc tttcgtgtgg
     8641 gaggtggtaa aagacgtgga gcagcccggc tcgagctatt attatccctt aggctatacg
     8701 accaactgga cccgcgccga cttcgggccg ctctggattc cggagaaagg tgccaccctc
     8761 acgttcgaca ctgatgtggc ctttaaagcg gcagcctacg aacgttgtat aaagaattac
     8821 gaagggaacg attttgcctt tcgcgacgga aaagtataca taaacgggga gccggcagac
     8881 tcgtatacct ttaagatgga ttactacttc atgatggggg ataaccgcca caactcggcc
     8941 gactcacgcg tgtggggatt tgttcccgaa gaccatatcg tgggacaacc gatgctgata
     9001 tggctttccc tggaaaaaga caacggctgg cttaacggaa aaatccggtg gaacaggtta
     9061 tttcgaagtg caaaaaaata accgggaggg tgttgctttc ttccttctac ctggcgcccg
     9121 tcgaatacta ttcggtattt tttcgcgcct ccagcaccgt tatagaggtt cacgaaaact
     9181 accagaaaca atcttaccga aaccggtgta acattgttgg ggccaacgga tcgatggcat
     9241 tgagcattcc tgtcgaaaaa ccttctgcag taaaatgccg gatgaaggat gtccgcattg
     9301 cagaccacgg aaactggcgg cacctgcatt ggaacgccat tgtatcggca tacagctcca
     9361 cacctttttt tgagtattat gcagacgaac tacagccgtt ctacgaaaag aggatccctt
     9421 ttctcgtcga ttttaacctt cagcttcacg aactcatctg cggatggctg cggatagagc
     9481 agccaactaa tctatctccg gaatacgttg ctgaaatacc tgaagggata gcggaccata
     9541 gagaagcaat tcatcccaaa cgaccctccg gcttcatgac caggccatac taccaggttt
     9601 tccaggataa actggggttc atccagaatg caagtatcat cgatttggtg ttcaatatgg
     9661 gcaacgaagc gaggttgtgg ctgtgaatca tacccgggtc ttattttttt cttacctttg
     9721 tagcctcaac atcaaaatta tgagcagaaa aacaagagtc cgcttcgccc ccagtcctac
     9781 cgggccgctt catatcggcg gagttcgcac ggcattatac aactacctgt tttccaaaca
     9841 acaaaacgga gacttcatcc ttcggattga agacaccgac tccacccggt ttgttcccgg
     9901 agcagaagca tacattcagg aagctttcga ctggttggga ctgccctacg acgaaagtcc
     9961 cgcaaaagga gggaaattcg ggccctaccg acagtcggaa cgaaaagaca tttacaaaaa
    10021 atacgtccac aaactgttgg acgaaggcaa ggcgtacatc gcctttgata ccccccagga
    10081 gctggaagcc aaacgcaacg aaatcccgaa ctttcaatac gatgccacca cccggcttca
    10141 gatgcggaat tcactgacgt tacccaccga agaggtgaaa aagctgatcg acaacggtac
    10201 gcaatacgtg gtacgcataa agattgagcc ccgtatcgat atcaccgtaa acgatttaat
    10261 tcgtggagaa gtggtaatca actcctcagt gctcgatgac aaggtcattt acaaatcggc
    10321 cgacgaactg ccgacgtacc accttgccaa cgtggtggac gaccacctga tggagatcac
    10381 gcacgttatt cgcggtgaag aatggttgcc gtcggctccc cttcacgtgt ggttatacag
    10441 aagtttcgga tgggaagata ctatgcccga gttcgcccac ctgccgctcc tgctcaaacc
    10501 cgaaggcaac ggaaagctga gcaagcgcga cggggaccgc ctcggatttc ccgtctttcc
    10561 gttggaatgg aaagaccgcc aatcaggtga aacatcatcg ggataccgcg aaagcgggta
    10621 cttgcctcag gccgttatca actttttggc tttgctggga tggaatccgg gaacagagca
    10681 ggagattttc tctctcgatg agctggttac ggccttttcg ttcgaacgat gcagcaagag
    10741 cggtgcaaag tttgatttcg aaaaagccaa gtggttcaac cacaaatata ttcagggaac
    10801 acccaatgaa ctgttggcgg agatgatcca acctgttttg gcagaaaaaa acattccctg
    10861 cgacaagaac tatgccgcca acgttatcgg attggtgaaa gaacgggtga atttcgtgaa
    10921 agaattgtgg gatcaggcct cgtttttctt cattgcgccg gcttcatacg acgaaaaaac
    10981 ggtaaaaaaa cgctggaaaa aggaaagttc cgcacaactg tccgaactta ttaacgtgtt
    11041 gcaaagcatc gatgacttct cttccgaaaa tcaggagatc cgggttaagc agtggattga
    11101 agagaaaaac tatcccatcg gaaacattat gaatgctttc cgtctggctg tagtaggcga
    11161 gagcaagggc cctcacatgt tcgacattac ttcgcttctg ggcaagcagg aaaccatagg
    11221 ccggttaacg acagccataa caaacattcc tgctccggag aatacataaa cccaatattt
    11281 gtacaaccaa aaactattcg aagttatgaa cctgaagtca aaaagaaaat tcagactaag
    11341 aacccgtttt tttgttcctg ctgttttcat cctggcaatt gctctggtta cactgctgtt
    11401 tccccgccag ggagatttca agtactcctt cagcgaagga agaccctggc aatacgggct
    11461 tttgactgct ccgttcgatt tccccattta caaacctgcc gatcaactga aagcagaacg
    11521 agacagcatc ctgcgctttt acgaacccta ctataccatc gatgaaagtg ttgagaagaa
    11581 cgcaatggct gaattcgatg ccgatgttaa cctgaacacg aagctcagca gcctgtctcc
    11641 tgattatatc ctttacctga ggaacagttt acagaaaata taccggtcgg gaatcatgcg
    11701 ttcagaggat tacgacaagg ttttcagttc cgaaactcaa tccatgcggt tacgaaaagg
    11761 aaacctggct gaatcaaaaa gtgtggaaac atttagcacc attaaatcgg catacgaaca
    11821 actcctcaat aatacgccga aaagcatgga tgccgaatta atacgtttgg cagatgtaaa
    11881 taaatatatc cgcgaaaata tcgtgtacga tgcttctaca tccgaaaagg ccagggagga
    11941 atttatccag caggtttctc cctccacagg gatggtgcaa acgggacagc gtattattga
    12001 ccaaggggaa atagtcagct cccaaaccta caaggtattg aactcactaa aacgggtcac
    12061 cgaagagcga agcgggagaa ccggtaaaaa cggatggatg attttcggtc aactcttgct
    12121 ggtagttctt ctgtttgggg cattttatgc atacctgctt ttcttccggc cgcatgaata
    12181 ccgaaacagg aagcacgtca cgttcatggt gttgctggtg acctcctttg tagcactgac
    12241 agcaatcacc agtcagcttg accttttcaa cgtgtacatt atcccgtatg ccatagtcac
    12301 tattctgata cgaacattta ttgactcccg tacggcattg tttgccagtt tgattaccat
    12361 tatcctaagt tcgctgatgg tcccctttcc gtttgaattt atcgtcatac agattgccgt
    12421 tgcgatggtg tcggtattta tgctgaaaga gctctcggaa cgttaccagt taatacgcag
    12481 ctccttcttt atcctgatcg cctacagctt gatgtatata ggcctggtca tgcatcagga
    12541 agggaacatc aacaaaattg acccgatcat atttatctat tttttcatca atttcatttt
    12601 tatattgttc tcctattcgc tggtatacct catcgagaaa tccttcggat tcatatcggg
    12661 ggtaagcctg attgaattat cgaacatcaa caaaccgtta ttgaaggagt tgtctgaaaa
    12721 agcaccagga accttccagc attcattaca ggtctcaaac ctgggtatgg ccgctgcagt
    12781 aaaaatcggg gccaatgctt ctctaataag gacgggagca ttgtatcacg atatcggaaa
    12841 aatggtaaat cctgcttttt ttaccgaaaa tcaatcgccg gggatgaatc cgcacgcggg
    12901 actccctttc gaggaaagtg cgcgcatcat catcaatcac gtgagagacg gagtgaaaat
    12961 agcgcaaaaa aacaacttgc cgaagcagat aaccgatttt attgaaacgc accacggaac
    13021 cagtatgccg aaatacttct acatctcctg gaaaaacgct aaccccggga aagagataaa
    13081 cgaagacctg ttccgctatc ccgggcccaa tcctttctca aaagagacgg ccatcatgat
    13141 gatggccgat gcggtggaag ctgcttcaag aagcttgccg gaatacaacg aagacagcat
    13201 ccggaatctg gtggaatcgc tgatcgattc acaggtgaca gaagggtact tcaagctggc
    13261 ccccatcact ttccgtgaca ttgcggccat aaaagaagtt tttcttcaaa agctccagac
    13321 catctaccac acacggattg cctatccaaa gctggacaaa ccccagactc aaccagaatc
    13381 agtataaagg tttttattcc cattcaatgg ttgccggcgg tttcgatgag atatcgtata
    13441 ccacgcggtt tacccctttg accttgttga taatctcgct cgacactttt gccagaaact
    13501 cgtaaggcag atgtgcccaa tccgctgtca tggcgtcagt ggaagttaca gcacgcaaag
    13561 caatggtatt ttcgtaagtg cgttcgtcac ccattacgcc caccgattgt accggtaaaa
    13621 ggattgctcc tgcctgccaa accttgtcgt acaaccctgc tgcacggagg ttcgaaacaa
    13681 aaatgtcatc ggcgtcctgt gctatgcgta ctttttccgg cgtgatgtcg cccaggatcc
    13741 ggatacccag tcccggaccg gggaaaggat ggcgataaat aagatgcggt ttcattccaa
    13801 gttccagacc gattttccgc acttcgtcct taaaaagtaa tttcaacggc tcgcacagtt
    13861 tcaggttcat tttctcggga agcccaccta cgttgtgatg gcttttgata acggttcccg
    13921 taatggaaag cgactcgatg atatcgggat aaatggtccc ctgcgccaac cactttatgg
    13981 cggtaagttt atgcgcttct tcgtcgaaga catctataaa tcccttgccg atgattttac
    14041 gtttgcgctc ggggtcagtt actccggcaa gttccttgta aaagcgttct cgggcatcta
    14101 caccgatgac gttcagtccg agatgttcgt aattttcgag aacggtttca aactcatttt
    14161 tacgcagcaa tccatgattt acgaaaatac aggtcaggtt ctttccaatg gctttgttca
    14221 gtaaaacggc tacaacggaa gaatccacac ctccggaaag ggccaaaatt accttatcgt
    14281 ctcccagtgt ttctttcaga tcagctactg tcgcctcaat gaacgatgca ggcgtccagt
    14341 ctttccggca tccgcagatc gccagaaaat tatccagtag cttgttacct tgttccgtat
    14401 ggaagacttc cgggtgaaac tgcactcccc atgtaggctc atccttcact ttgtaggcag
    14461 cgatttccac ctcttcggtg gaagcaataa ccgagaaatt atcgggaatc cgcacgatcg
    14521 tatccccgtg agacatccat acttgcgatt gttgcgggag gttccccaaa agcaggtcac
    14581 ctgtttcttt cacgctcaga tgggccctcc catattcgcg cgaatcgctc ttgtcgaccg
    14641 taccgcccga tgattgtgct atccactggg caccgtaaca aattcccagt accggataat
    14701 tacgccgtat ttcatggaga tcggttttaa aagcattgtc gtcgttgacc gaaaacgggc
    14761 ttccggagag aattactcct tttaccgatc tatctccgaa agggaatttg ttgaagggta
    14821 ctatttcgca ataagtattc agctcgcgta cacggcggcc gatgagctga gttgtctgag
    14881 aaccgaaatc gagaatgatt attttttcgt gcatatattt ttgaagaagt tagaaattag
    14941 aagccagaaa ttagcatgaa cacacaagca acagcggcgc gttgtttgta ttttttgcag
    15001 tgcaaagata cgaaaagtat ggaaaaaaat agcatgacgt atggtataag catcggatag
    15061 ataaattaac aaaagaagat cattttacaa attattttcc tatctttgaa tctttgaaga
    15121 tcatatttca gactgataat gaaagtacac caccaattat ttgcattcat tcttttaatc
    15181 attcccgttt tatcgatggg ccaatcggtt gatgatgcga aaaaatggta cctggaaggg
    15241 gactttacgc aagcaaaacc tgtttttgaa gctgaatacc gggttaatcc caacaatgcc
    15301 cagcttaatc tatggcttgg tgtaatatcg cttgctgaaa gcgattatca aaaagcccag
    15361 caataccttg agtttgcttc gctaaaaaat attcaggagt cgtacctaca actggggcag
    15421 ctttacacta aaatgtaccg gtttgccgac gctgaaaagg aattcacccg atacgaaaaa
    15481 gccaaccgga gaaataagca agcgcttgca ttactcaacc cttacaaaga atatgccgat
    15541 aagctgaaac gactgataaa ccgcactgag gatgttcaga tcattgacag catagttgtc
    15601 cctaaatccg attttctctc ggtttataaa ctgagtgcat ctgccggatc gctgcagccg
    15661 gtaagtggtt ttttccggga tcaacccaac aacaatcagg tgcttttcct caacgaacgt
    15721 caggataaga tctactatcc gcaagacgat tcgatttcgg gaagtaacct ctataccatg
    15781 gaaaaactac tggacagctt cggcaatgag aaacggttat cggaatcgat caatcaagag
    15841 gggaatcagg cttacccgtg tgttatgccc gatggcctta ccatttattt ctcctccacc
    15901 gggcacgttt cattgggtgg gtatgagatc ggaagagcac ac
//
LOCUS       DAOSDR010000014        15837 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrimonas sp. isolate AMR_MDS_1826
            NODE_1493_length_15837_cov_3.852427, whole genome shotgun sequence.
ACCESSION   DAOSDR010000014 DAOSDR010000000
VERSION     DAOSDR010000014.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863355
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrimonas sp. (wastewater metagenome)
  ORGANISM  Petrimonas sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Dysgonomonadaceae; Petrimonas.
REFERENCE   1  (bases 1 to 15837)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15837)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:10:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,720
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 35
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15837
                     /organism="Petrimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1493_length_15837_cov_3.852427"
                     /isolate="AMR_MDS_1826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2023866"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1743)
                     /locus_tag="PLI30_01140"
     CDS             complement(<1..1743)
                     /locus_tag="PLI30_01140"
                     /inference="COORDINATES: protein motif:HMM:TIGR04056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0071702 - organic substance transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SusC/RagA family TonB-linked outer membrane
                     protein"
                     /protein_id="HOI78190.1"
                     /translation="MKISALLFFLCTFCVLGEDIYSQNNEVTLNLKNVTIKDAISEIE
                     KETGYVFIFNEDVEIRLKGRVTIDVGSKPINTVLSQLLRGKDLDYNIIAKQITLFVNN
                     KKGNIYVPTSQVALKIEPQQQGNTVKGKIVDERGEPLPGVTVVVKGTSIGTTSDVDGN
                     YVLHMVSKNSVLTFSFVGMRTQEVEVGNQTTINAIMAEETIGLEEIIAVGYGTIRKRD
                     VSTSIASVTSESLKDKPVSSYLQAMAGKLAGVRIFTSNNVPGGGSNVRIRGVNSINAS
                     NAPLTVIDGFPISDGYNQTENLFGSINTSDIESIEVLKDASSSAIYGARAANGVILIT
                     TKKGKIGKPTISVNISTGFENMINKLDVLNREDYLSFVEDSRSQAYIIEDPGYGSDNP
                     NVSNWSWSDNTALRLDNMMKYSSVAGPMQQPGNLYQRWYTIIPEVYAQPYDTDWQDVG
                     TQTGMVQDIQLSATGGTDNLKYMISGGYFNQEGIMEATGYERYSLRSNIELKINNWLK
                     TGLLLAPSMENTRLPNAPETYFQSLITYIPLLAPYDENGDPSWLGYFPGQPGPWNWME
                     WNMGAGVNPLVNSLI"
     gene            complement(1949..3088)
                     /locus_tag="PLI30_01145"
     CDS             complement(1949..3088)
                     /locus_tag="PLI30_01145"
                     /inference="COORDINATES: protein motif:HMM:NF016648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR domain-containing protein"
                     /protein_id="HOI78191.1"
                     /translation="MKENRFTYNPYFLENKKFIQWRLTRVPELEHYWNSFIRENPDHV
                     EEFQKAIEIFDKIAINKRTFKDTDILYGRVVESALRKKQREKRIIYSVSSVAAVALVL
                     FITTFYFVWRNDNSEPATTSTEIVGKTLPEQDVKLIAGGKELKLNQNVTLQLKNGQIS
                     YTDSTDTEKRVEVNNIQTNKLIVPDGKRSFLILADGSKVWINSGTELEFPTKFSGKTR
                     EIMVSGEIYIDVVQSAKQPFIVRTPDLDVQVFGTRFNVSAYASDKTSAVVLVNGKVEV
                     KTKDDRSIRMNPNEMVEFRNGNMNKENVDVSLYTSWVEGVFIFNRTPVSELFKKVGRY
                     YNVSFENVESDKRITGKLYLSENLHDVISSISLLTGKKYMIKAIS"
     gene            complement(3227..3844)
                     /locus_tag="PLI30_01150"
     CDS             complement(3227..3844)
                     /locus_tag="PLI30_01150"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOI78192.1"
                     /translation="MQEKNKHIAIDNCWKEFLANKDGYDAFSFIYDSYVHDLLSYGVN
                     LGFDEDTCRDAVHDVFCKILSDKNKFLNVNNLTSYLFRAVRNYLFNFQKKNHNLSDSS
                     FENIPFFTEVTTLESLIDREDTEKLKQTVENLLNQLTPRQREVVYLRYMQEMDYEEIA
                     VLMEINYNSARRLVHRSIEHLRSVNKDSEKPLFVILVALTYYHYL"
     gene            4092..5024
                     /locus_tag="PLI30_01155"
     CDS             4092..5024
                     /locus_tag="PLI30_01155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019539911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HOI78193.1"
                     /translation="MLNSLESNTYKNPVINYSLPDPTVIKANDGFFYLYATEDIRNTP
                     IYCSQDLVNWKFVGTAFTDATRPTFEPKGGLWAPDINYINGKYVLYYSMSVWGEEWTC
                     GIGVATSDKPEGPFTDHGKLFRSSEIDVQNSIDPFYIEENAKKYLFWGSFRGIYAIEL
                     SDDGLSVRPGAEKQRIAGTAYEGTYIHKRNGFYYLFASVGSCCEGLNSTYTTVVGRSE
                     NLFGPYVDKQGQSMMDNHHEVMIRKNQKFVGTGHNSEIVQDQEGQDWMLYHAVSVDNP
                     KGRVLMMDQVRWKDEWPYVEGNTSSLTSPKPIFN"
     gene            5040..7079
                     /locus_tag="PLI30_01160"
     CDS             5040..7079
                     /locus_tag="PLI30_01160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011108528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI78194.1"
                     /translation="MKIKDIARYALLVLFIVSTVQATARVAENNFSSSIALKIPGNNA
                     AHYPLQEIRKGDVVQLKANQNIPVVITRTIVSPNLSAVDHVVEVVITAKENIYFNFKQ
                     WVNTRYSHNDCQFYMPGFWYRRNLRSPKEAPSFHTSDSWTVREDRLSVPMTAIFNEKS
                     GTYLSVMRMDDFKHEALTTHKEGEVIISGKTSIGYTGFENKNGDARISFGFPYQETPK
                     TYIRKLTLAPPVEAFQFLEKGKSIRLTWLVKSGKASDFSDFVRQSWEYSYDAWLPGPV
                     NTPFSDAFVKKTLTGFFTRSFVSDYPLKFNSGEGLVVATCESLARAEIGFVGRVLLNA
                     FNALEYGQQNNRSELVANGYSVFDSYLEHGFTANGLLREHVYYDKNTEASNLSIRRQS
                     EGVYAMFQFLQYEKNNGRRHPEWETKLQTLLDRMLLLQNEDGSFPRKFTDNLVISDAS
                     GGSTPSVTLPLVMAYRYFNNPKYLTAAQKTADYLEREIISKADYFSSTLDANCEDKEA
                     SLYAATAMYYLALISTGEEQQRYADLCLKASYFALSWYYLWDVPFAQGQMIGDNGLQT
                     RGWGNVSVENNHIDVFIFEFGSVLNWLSKRYNEPRFHDFVQVISSSMRQLLPFENHMC
                     GVAKTGYYPEVVQHTNWDYGKNGKGFYNDIFAPGWTVASLWELLSPGRAENFLSK"
     gene            7195..8319
                     /locus_tag="PLI30_01165"
     CDS             7195..8319
                     /locus_tag="PLI30_01165"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011108863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase family protein"
                     /protein_id="HOI78195.1"
                     /translation="MAVAAIIACTSNPKEAPGEPSTLSGLKRSHFQSSVNGDSTDLFV
                     LKNANGMEVTVTNYGGRIVSVMVPDKTGTLQDVVLGFDSIQGYTSVSNNLGATIGRYG
                     NRIANGKIKVAGVEYQLPQNNYGHTLHGGPEGYDTKVFRAIQPDSQTVVLTYLSKDGE
                     AGFPGNLNVKVTMNLTNDNAVDIQYEADTDKETVVNLTNHSYFNLSGDPNNTVLDHLL
                     TIYADGYTPVDDTFMTTGKIDPVKDTPMDFTVATPIGQRIEDTTFVQLKNGKGYDHNW
                     VLNAKGDVTQVAATVVSPVTGIQLDVYTSEPGLQVYTGNFLDGTMTGKKGIVYNKRSA
                     ICLESQKYPDSPNKPDWPSSYLKPGEKYTSRCIYKFSVNL"
     gene            8350..9138
                     /locus_tag="PLI30_01170"
     CDS             8350..9138
                     /locus_tag="PLI30_01170"
                     /inference="COORDINATES: protein motif:HMM:NF019590.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NIPSNAP family protein"
                     /protein_id="HOI78196.1"
                     /translation="MKTKLLLPLFVILFIVPQFIRSQSKGQPREFYQLTVYHYNTIDQ
                     EKIIDNYLQGALIPALHEMKIKSVGVFKAIANDTSANKTLYLFVPLKSLNAVDEINTR
                     LMRNNKFNANNKEYADVLHNNPAYNRMETILLKAFPLAPAMKLPNLKSAKKQRVYELR
                     SYESASEKIFRNKVHMFNEGGEMEIFTRLNFNPVFYSEVVAGGKMPNLMYMTTFENKE
                     DRDAHWDAFRVDPAWKRMSSLPEYQNNVSHSDITFLRPADYSDF"
     gene            complement(9123..9788)
                     /gene="nth"
                     /locus_tag="PLI30_01175"
     CDS             complement(9123..9788)
                     /gene="nth"
                     /locus_tag="PLI30_01175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007652955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HOI78197.1"
                     /translation="MTKKQRFAYVMDWFLENAESSETELHYANNYQLLVAVILSAQCT
                     DKRVNLISPRVFEAFPTPEVMAASSPDEVFEYIKSCSYPNNKAKNLVGMARKLVSDFN
                     GEVPSTIDDLLTIPGVGRKTANVMLSVAFDLPAMAVDTHVFRVSNRIGLTDNSKSPLE
                     TERELVKYIPERLLTRAHHWLILHGRYICIARKPKCGICGLRDWCKYYQREVVVGLSQ
                     KSE"
     gene            complement(9794..11359)
                     /locus_tag="PLI30_01180"
     CDS             complement(9794..11359)
                     /locus_tag="PLI30_01180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019539906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M81 family metallopeptidase"
                     /protein_id="HOI78198.1"
                     /translation="MKKLFSTSLLLAILISAVGCQNHKKKLPRIAIAGLAIESSTFSP
                     AVSHEDAFRARYGDSVFSYYPFFAADSGIIDRAVWLPTLRGHAMPGGIVSREAYESLV
                     SKTLDSLKAKLPLDALFFDVHGAMSVQGLDDPEGDFLVRIRELVGTDVLISTSMDLHG
                     SVSPRLAQNTDLITCYRLAPHEDAIESKKRAVTNLLDRLENGKGKPAYKAWIPVPILL
                     PGEKTSTRIEPGKSLYAQIPEVIEKDKVIDASIWMSYPWADEPRNHGVVVAYGDDKEA
                     VGKAAEKLAAHFWNVRKEFDFVAPTVYLDEALEKALASDKKPFIISDMGDNPTAGGAG
                     DVTWTLQEILKRPELKSPRAKSLIYASIPGEEFVKKAKEIGVGGKIDATAGAAVDNRF
                     APPVRITGTITAIHEGHPNAKTEVVVRNGNTSVIVTERRMAYHYEKDFTKLGLNPRET
                     DILVVKIGYLVPELYDMRADWLMALTPGGVDQDLLRLDYKRINRPIYPLDPDMADPDL
                     NATFIPLSDEIKR"
     gene            complement(11410..12546)
                     /gene="chrA"
                     /locus_tag="PLI30_01185"
     CDS             complement(11410..12546)
                     /gene="chrA"
                     /locus_tag="PLI30_01185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002978301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate efflux transporter"
                     /protein_id="HOI78199.1"
                     /translation="MKETPKPILKELAALFLKLGIIGFGGPAAHIAMMQQEVVEKRKW
                     LTEQEFLDLLGATNLIPGPNSTEMAIHIGQEKGGWRGLIIAGACFIFPAVLIVGFLAW
                     LYKDYGQLPQVQPFIYGIKPAIIAVILGAVFPLAKKSIKSVELAILGILVLVLSLLNI
                     NEIYLMFGAGLLAMLVARIRNRQKIKFNGIVPLALLPPTVPVLLSVSNVNLFLIFLKI
                     GAILYGSGYVLFAFLDTELVTTGFMSRQQLIDAIAVGQFTPGPVFTSVTFIGYQINGF
                     WGAVVSTVGIFLPSFVFVALLNPVVKKMRKSTLFSLFLDAVNVASVAIIAAICLIMGK
                     ETITDMRTVVIAVLSMAVVFRYRKVNSAFIVIGGCLAGYLLSLI"
     gene            complement(12552..13259)
                     /locus_tag="PLI30_01190"
     CDS             complement(12552..13259)
                     /locus_tag="PLI30_01190"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006841547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HOI78200.1"
                     /translation="MADTVFYSPGIRHLQQLPEQESLHCVKVLRMKEGDKLTVTDGKG
                     FFYPCTLIQAHPKHCVVSMDAEVAQPKTWNFSLHIAFAPTKNIDRMEWFAEKATESGI
                     DRFTPLLCRFSERKELKTERLEKIVVSAMKQSQQAVMPVIDEMNPFDLFVENPFTGRK
                     FIAHCHPTPKTALAKTYQKGEDALILIGPEGDFSEEEIQKAVSLGFEPVSLGENRLRT
                     ETACLVACHTLHVLNLT"
     gene            complement(13252..13854)
                     /locus_tag="PLI30_01195"
     CDS             complement(13252..13854)
                     /locus_tag="PLI30_01195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019539902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nuclease family protein"
                     /protein_id="HOI78201.1"
                     /translation="MTNKVKLSILGISFSQVQAGAYALIFAEENGIRRLPIVIGTPEA
                     QSIALVMENMTPPRPLAHDLIYNIFESLSIELLEVFIYKFEDGAFFAELLLRQDNREF
                     RIDSRTSDAVAIALRTHSPIFTTETIMQNMAIVFDEKEVLSTEFQNKDMEVEEIVENM
                     EEVKIDALKKRLEEAIKDEDYELATKLRDEINRRQGAENG"
     gene            13998..14073
                     /locus_tag="PLI30_01200"
     tRNA            13998..14073
                     /locus_tag="PLI30_01200"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:14033..14035,aa:His,seq:gtg)
     gene            complement(14172..14759)
                     /gene="hisIE"
                     /locus_tag="PLI30_01205"
     CDS             complement(14172..14759)
                     /gene="hisIE"
                     /locus_tag="PLI30_01205"
                     /EC_number="3.5.4.19"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019539136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_function: GO:0004636 - phosphoribosyl-ATP diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoribosyl-AMP
                     cyclohydrolase/phosphoribosyl-ATP diphosphatase HisIE"
                     /protein_id="HOI78202.1"
                     /translation="MTINFKKSGGLVPVIIQNTHTLQVLMLGYMNEEALEKTKAEGRV
                     TFFSRSQNRLWTKGETSGNYLIVNEIREDCDNDTLLIKALPQGPTCHTGATTCFSEET
                     PKGFIYELEKVIEQRIEAKTEGSYTSRLFNSGVNKVAQKVGEEAVELVIEAKDNNIDL
                     FKNEAADLLYHFLILLKTKALKLEDIEGVLKGRHK"
     gene            complement(14774..15529)
                     /gene="hisF"
                     /locus_tag="PLI30_01210"
     CDS             complement(14774..15529)
                     /gene="hisF"
                     /locus_tag="PLI30_01210"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016277827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HOI78203.1"
                     /translation="MLKKRIIPCLDIKDGRTVKGINFVGLRDAGDAVELAKRYVQEGA
                     DELVFLDITATVEKRKNLTGLVEKIAREINIPFTVGGGINSVEDAQAIIKAGADKVSI
                     NTAAVDHPEIVSEIAAKFGSQCVVLAIDTKLDDGEWKVYIHGGRTPTPLNAVDWALEG
                     ERRGAGEILLTSMNNDGTKNGFALDITAAVSSAVNIPVVASGGAGTMQHFADVFQQTR
                     ASAALAASIFHFGEIPIPRLKQFLKQQNIEVRI"
     gene            complement(15562..>15837)
                     /locus_tag="PLI30_01215"
     CDS             complement(15562..>15837)
                     /locus_tag="PLI30_01215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019539138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HisA/HisF-related TIM barrel protein"
                     /protein_id="HOI78204.1"
                     /translation="VVDFIAGYERKGVTYVICTDISKDGMLQGTSNELYAEILRETDV
                     KLIASGGVSSVDDLTKVKELGCEGAIIGKALYEGRISLDELRVAGFL"
BASE COUNT         4293 a         3685 c         3395 g         4464 t
ORIGIN      
        1 aattaaactg tttaccagtg ggttaacccc agcacccata ttccattcca tccagttcca
       61 agggccaggt tggccgggga aataacctaa ccacgagggg tctccgtttt catcatacgg
      121 tgccaacaaa ggaatgtagg tgatcaagct ttgaaagtaa gtttccggag cattgggcaa
      181 tcgggtattt tccatactgg gagctaacaa tagtcctgtt ttcaaccagt tgttgatctt
      241 taattcaata tttgatctca aagaataacg ctcataaccg gtcgcttcca tgattccttc
      301 ttgattaaaa taccctccgg aaatcatata cttcaagtta tcggttccac ccgtcgccga
      361 caattgaata tcctgtacca ttccggtttg agtgccgaca tcctgccagt cagtgtcata
      421 aggttgggca tacacttcgg ggattatggt gtaccagcgc tgatacaagt tacccggctg
      481 ttgcataggc ccggcaacgg acgagtattt catcatattg tccaaccgga gcgcagtatt
      541 gtcgctccac gaccaattcg aaacattcgg attatccgaa ccatagcctg gatcttcaat
      601 aatataggct tgagaacgtg aatcttcgac aaaacttaag taatcttccc ggttgagaac
      661 atccagttta ttgatcatat tctcaaaacc ggtactgatg ttcacgctta tggtaggctt
      721 tccaatcttt ccctttttgg tagtaatcag aataaccccg tttgccgccc gggcaccata
      781 aatggcactt gaagaggcgt ctttcagcac ttcaatagat tcaatatcgc tcgtattaat
      841 ggaaccgaac aaattttccg tttgattgta accgtcggag atggggaatc cgtctataac
      901 cgtcaaagga gcattagacg cattgattga gttaacgccg cgtatgcgca catttgaccc
      961 gcctcccgga acattgttgg acgtaaaaat tctgactccg gccaattttc ctgccatagc
     1021 ttgtaaataa ctggagacag gcttgtcttt caaactttcg gaagtaacgg atgctatgga
     1081 tgtcgaaaca tctcgttttc taatggtgcc atacccgaca gctataattt cttccaaccc
     1141 aattgtttct tctgccataa tagcatttat ggttgtttga tttcctacct ccacttcttg
     1201 agtgcgcata cccacaaacg aaaaagtgag aactgaattt ttagaaacca tatgcaaaac
     1261 ataatttcca tcaacatcac tagtggtgcc tatcgatgtg ccttttacta ctacggttac
     1321 ccccgggaga ggctcaccgc gttcatctac aatttttcct ttaaccgtgt taccttgctg
     1381 ttggggctcg atcttcagag ctacctgact tgtaggtaca tatatatttc ccttcttgtt
     1441 gttgacaaaa agtgtgatct gtttcgctat tatattgtaa tctagatcct ttcctctcaa
     1501 taactgactt aaaacggtat ttatcggttt acttccgaca tctatagtta cacgcccttt
     1561 caacctgatc tcaacatctt cattgaatat aaaaacgtaa cctgtttctt tttctatttc
     1621 agaaattgca tctttgatcg taacattttt cagattcaaa gtaacttcat tgttttgcga
     1681 ataaatatcc tctcccaata cgcaaaacgt acacagaaaa aacaataacg cagatatttt
     1741 catgattttt agaactctat tatatctttt tggtataata atttcattgt taattttttg
     1801 tttcatacat ttgtcataga ttttagttta cgaaataatt tggattatct tgtgaattaa
     1861 tttttctggt cgagtggtgc ggcaacatct ctcggccttc ttttttttta tctatccata
     1921 agcaacattt tttatttgtt tataactttt atgatattgc cttaatcata tatttttttc
     1981 ctgtcaaaag ggaaattgaa gatatgacat catgcagatt ctccgataaa taaagttttc
     2041 ccgtgattct tttatcactt tctacatttt cgaaagagac gttgtaatag cggccgacct
     2101 ttttaaataa ctccgaaacc ggagtccggt taaatataaa tactccttct acccaacttg
     2161 tgtataaaga aacgtccaca ttttctttat tcatattccc attgcgaaat tcaaccattt
     2221 cgttaggatt catccggatt gatctatcgt ctttggtttt cacttctact tttccgttta
     2281 ccaggacaac cgctgacgtt ttatccgaag cataggccga gacgttaaag cgggttccga
     2341 aaacctgaac atccagatcg ggggtacgaa cgataaaggg ttgtttggct gattggacaa
     2401 catctatgta gatttccccg gacaccatta tttcacgtgt ttttcccgaa aactttgtag
     2461 gaaattccaa ctccgttcct gaatttatcc agaccttact tccatcggca agaataagaa
     2521 aagaacgctt cccatcgggg acaatgagtt tattcgtctg gatattatta acttcaactc
     2581 ttttttcggt atcggtacta tcggtatagg aaatctgccc gttcttcaac tggagtgtta
     2641 cattctggtt cagttttaac tcttttcctc ccgctatcaa cttcacgtct tgctcgggaa
     2701 gagttttacc aacaatttcc gtactcgttg ttgcgggttc cgagttatcg ttcctccaaa
     2761 caaaataaaa agttgtaata aaaagcacca aggcaacggc ggcaacactt gaaacagaat
     2821 aaataattct tttttctctt tgtttcttcc gcagggcact ttcaacaacc cgcccgtaca
     2881 atatatccgt atccttgaac gtccgtttat taattgctat tttatcgaat atctcaatag
     2941 ccttctgaaa ctcttctacg tgatcgggat tttcccgtat gaaagagttc caataatgtt
     3001 ccagctcggg tacacgcgtg agccgccatt ggatgaattt tttgttttcc agaaaataag
     3061 ggttgtatgt aaatctattc tccttcattt ttttagtttt actcattcac atgaattaac
     3121 ttaactaact tataaaacat atatatacat ataagagaca atcgaacttt tcttttgtag
     3181 tcaaaaacaa aagaaaaaat aaaaatttaa cattaaagaa gggagtttac agatagtggt
     3241 aataggtaag ggccaccaaa ataacaaata agggtttttc cgagtcttta tttacggatc
     3301 gtagatgctc aatactccgg tgaacaagcc ttcttgccga attataattt atttccataa
     3361 gtactgcaat ctcctcataa tccatctcct gcatgtacct caaataaaca acttctcttt
     3421 ggcgtggagt cagttggttc aatagatttt cgacggtctg ttttaatttt tctgtatctt
     3481 cccggtcaat gagagattcc aatgttgtga cttcagtaaa aaaaggaata ttctcgaagg
     3541 aggaatcgga taaattatga tttttctttt gaaaattgaa caaataattt cttactgccc
     3601 tgaataaata ggaagtcagg ttatttacgt taaggaactt atttttgtcg gaaagaatct
     3661 tgcaaaatac gtcgtgaacc gcatccctgc aggtatcttc atcgaaaccc aggtttaccc
     3721 cgtatgataa gagatcatga acataggagt cgtagatgaa agaaaaggcg tcgtatccgt
     3781 ctttattggc taaaaattct ttccaacaat tatcaatcgc aatatgctta tttttttctt
     3841 gcattcttat ctaaaatctt gtcattcatg tgatgagcaa gaaatgttaa gacaaaatta
     3901 aacaattaat tttatatatt cacttttttt atttattaag cttgttgatt aagttaaaca
     3961 attgaataag tgtaaaactt tcgtttatac aaatctcttt tatacttttg taaaaaaaca
     4021 gttttatgtt tcatcctatt gaactattcc tacttttgct tctgctttcg tgtaaagcta
     4081 atggagcagt aatgttaaat tcgctggaaa gcaacaccta taaaaacccg gtaattaact
     4141 acagtcttcc cgatccgaca gtgatcaaag ccaacgacgg attcttctac ctttatgcga
     4201 ctgaagatat ccggaatact cccatttatt gttcgcaaga tttagtgaac tggaaatttg
     4261 ttggcactgc atttaccgat gcaacccgtc cgacatttga accgaaaggc ggtttgtggg
     4321 cgcccgatat caattacatt aacggaaagt atgtgctgta ctattccatg tcggtatggg
     4381 gtgaggaatg gacctgcggg ataggcgtgg ctacctccga taaacccgaa ggcccgttta
     4441 ccgatcacgg caagctgttt cgcagcagcg agatcgatgt gcaaaattcc atcgatccct
     4501 tttatatcga agaaaacgcg aagaaatacc tgttctgggg cagctttcgg ggcatttacg
     4561 ccatcgaact ttcggacgac ggactcagcg taagaccggg agccgaaaag caacgaattg
     4621 ccggcacggc ttatgaaggg acatacatac ataaacgaaa cggtttttat tacctgtttg
     4681 cctccgtcgg atcctgctgc gaagggttaa acagcacgta taccactgtg gtagggcgtt
     4741 cagaaaatct ttttggccct tatgtggata agcaaggaca gtccatgatg gataatcatc
     4801 acgaagtaat gatccggaag aatcaaaaat tcgtgggtac gggacataac tcggagatcg
     4861 tgcaggacca ggaaggacag gattggatgc tttatcacgc cgtctcggtg gacaatccca
     4921 aagggcgtgt cctgatgatg gaccaggtac ggtggaagga tgagtggccc tacgtggaag
     4981 gaaatacctc ttcgttaaca tctccaaaac caatttttaa ttagaaaaag aaatattgca
     5041 tgaaaattaa ggatattgca cggtatgctc tgcttgtttt gtttatagtg agcacggtac
     5101 aggcaacagc ccgtgtggct gaaaataatt ttagttcctc catcgctttg aaaataccag
     5161 gaaacaatgc ggcccactac cctttacaag aaattcggaa aggggatgta gttcaattaa
     5221 aagccaatca aaacatcccg gtagtgataa cacgaaccat cgtgtcacct aacctttcgg
     5281 ctgtagatca tgtggtggaa gtggtgatta ctgccaaaga aaatatctat ttcaacttca
     5341 agcagtgggt aaatactcgt tattctcaca acgattgtca attctacatg ccggggttct
     5401 ggtaccgccg caacttgcgc tcgcccaagg aggccccttc ttttcacacc tccgatagct
     5461 ggacagtgcg tgaggataga ttgagcgtgc ctatgacggc catatttaat gaaaaatcgg
     5521 gaacgtacct gtcggtaatg cgaatggatg attttaagca tgaagcactt actacccata
     5581 aagaaggaga agtaattatc tccggaaaaa catctatcgg ctataccgga ttcgagaaca
     5641 agaatggcga tgcccgtata tccttcgggt ttccttacca ggaaacgcca aagacctata
     5701 ttcgtaaact tacgctggct cctccggtag aagcattcca gtttctcgaa aaagggaaat
     5761 ccatccggct cacgtggtta gtgaaatcgg gaaaagcctc ggatttctcg gatttcgtac
     5821 gtcaatcgtg ggaatacagt tatgatgcct ggcttccggg ccccgttaac acacccttta
     5881 gcgatgcgtt cgtgaagaaa acccttaccg gttttttcac ccggagcttc gtgagcgatt
     5941 atcccttgaa gttcaattcg ggcgaagggc tggtagtagc tacctgtgaa agccttgccc
     6001 gcgcggaaat aggttttgta ggtcgagttt tgttgaacgc cttcaatgcg ttggaatatg
     6061 gacaacagaa caaccgttcg gagttggtag ccaacggcta ttccgttttt gacagttacc
     6121 tggagcatgg gttcacagcc aatggacttc tccgcgaaca tgtgtactac gacaaaaaca
     6181 cggaggccag taacttaagt attcgccgac aatcggaagg tgtgtacgcg atgttccagt
     6241 tcctccaata cgaaaaaaac aacggaagac ggcatccgga atgggagaca aaattgcaaa
     6301 ccttgttgga ccggatgctt cttttacaga atgaagacgg tagttttcca cggaaattca
     6361 ctgacaacct ggtcatcagt gacgcaagcg gaggaagcac cccttcggtg accttacccc
     6421 tggtgatggc atacaggtat ttcaacaacc ctaaatacct caccgctgcc caaaaaactg
     6481 ccgactacct ggaacgtgaa atcatttcga aggcagatta tttctcgtct accctcgatg
     6541 ccaattgtga agacaaggaa gcatccctgt atgctgctac agccatgtac tacctggcat
     6601 tgatttccac aggcgaagaa caacaacgtt atgccgacct gtgtctgaag gcttcttact
     6661 tcgcgctctc gtggtactac ctgtgggatg tgccgttcgc acaaggccag atgattggcg
     6721 acaacggtct acagacaagg ggatggggca acgtttcggt agaaaacaac cacatagacg
     6781 tatttatttt cgagtttggt tcggtgctca actggctctc aaaaagatac aacgaacctc
     6841 gttttcacga ttttgtacaa gttatttcgt cgtcgatgcg ccagttgttg ccgtttgaaa
     6901 atcatatgtg tggagtggcc aagaccggct attatccaga ggtggtgcaa catactaact
     6961 gggattatgg caagaacgga aaaggattct ataacgatat ttttgctccg ggatggaccg
     7021 tagcctctct atgggaactg ctctctcccg gcagggccga gaatttttta tccaaataaa
     7081 acagtttgta ggtgaaaatg atgtaaattt gtgttacacg attgaaccgg atactcattt
     7141 ttatataaaa aagaaaacaa catgatgaaa ctaaagtatt taacagcatg gattatggca
     7201 gttgccgcca taattgcttg cacatccaat ccaaaagaag ccccggggga accttcaacg
     7261 ttatcggggt tgaaaagaag ccattttcag tcgtcagtaa acggcgattc aaccgatttg
     7321 tttgtgttga aaaatgccaa cgggatggaa gttaccgtga cgaattacgg agggcgtatt
     7381 gtatcggtaa tggtcccgga taagaccgga accctgcagg atgttgtcct cggattcgac
     7441 tctattcagg gctatacaag cgtttccaac aatttgggag caaccatcgg acgctacggg
     7501 aaccgcattg ctaacgggaa aataaaggtt gccggtgtag aataccagct tccgcaaaac
     7561 aattacggcc acacattgca cggtggtcct gaaggttacg acacaaaagt ttttagggca
     7621 atacaacccg acagccagac agttgtacta acttatcttt caaaagacgg tgaagccgga
     7681 tttcccggaa acctgaacgt aaaagtcacc atgaacctga caaacgataa tgctgtcgac
     7741 attcaatacg aggcggatac cgacaaggaa accgttgtga atttgacgaa ccattcctac
     7801 ttcaacttga gcggagaccc caacaacacg gtactggatc atcttctgac tatttatgcc
     7861 gatggatata ctccggttga cgatacattt atgaccaccg gaaaaattga tccggtgaaa
     7921 gacacgccca tggattttac ggttgccaca cccatcggac agcgtattga ggataccact
     7981 ttcgttcaat tgaaaaacgg caaaggctat gaccacaact gggtgctgaa cgcaaaaggt
     8041 gatgtcaccc aagttgcggc cacggtggta tctccggtta ccggaattca actggacgta
     8101 tatacatcag agcccggatt gcaggtttac acgggaaact ttctcgatgg cacaatgacc
     8161 ggaaagaaag gaatcgttta caacaaacgc agtgccatat gcttggaatc gcaaaaatat
     8221 cccgattcac ccaacaagcc cgattggcca tcctcctacc tgaaacccgg ggaaaaatac
     8281 accagccgct gtatctataa gttctcggtg aacctgtgac acaaacattc tataatccag
     8341 ccccatccaa tgaaaacaaa acttttgctt ccgctatttg tcattctttt tatcgttccc
     8401 caatttatcc ggtcacagtc gaaaggacaa cctcgtgaat tctatcagct gacggtttat
     8461 cactacaata cgatcgatca ggagaaaatt atcgataatt acctgcaggg agcattgata
     8521 cctgccttgc acgaaatgaa gatcaagtcc gtcggcgtat ttaaagccat tgcaaacgat
     8581 acctccgcaa acaaaacgct ctatcttttt gttccgttaa aatcgttgaa tgcggtggat
     8641 gaaattaaca cccgtttaat gaggaacaat aagtttaatg cgaacaataa agagtacgca
     8701 gatgttcttc acaataatcc ggcctacaac cggatggaaa ctatacttct aaaagctttt
     8761 cccctggcac ccgcaatgaa gcttccaaac ctgaaatcag caaaaaaaca acgggtatat
     8821 gagttacgca gttacgagag cgccagtgag aaaattttcc ggaataaagt gcacatgttt
     8881 aacgaaggcg gggaaatgga gatatttacc cggcttaatt tcaaccctgt attctacagc
     8941 gaggtggtcg cagggggaaa aatgcccaac ctgatgtata tgacaacgtt tgaaaataaa
     9001 gaagacaggg atgcacactg ggatgccttt cgtgtagacc ctgcctggaa acggatgtct
     9061 tccttgcctg aatatcaaaa caacgtttcc cacagcgata tcacctttct gcgtccggca
     9121 gattattccg atttttgaga caatcccaca acaacttcac gctgatagta tttacaccaa
     9181 tccctcagcc cgcaaatacc gcacttgggt tttcgtgcga tgcagatgta cctaccgtga
     9241 agaatcagcc agtgatgggc ccgggtcaac aatctctctg ggatatattt gaccagttcc
     9301 cgttcggtct cgagtggtga tttcgagttg tccgtcaacc cgatccggtt agaaacccgg
     9361 aaaacgtggg tatccactgc catggcagga agatcgaaag cgacggacaa catcacgtta
     9421 gctgtttttc tgcccacacc gggaatggtc agcaaatcgt caatggttga cgggacttcc
     9481 ccgttgaaat cggagacaag ttttcttgcc attcccacca gatttttggc cttgttgttg
     9541 gggtaagagc acgattttat gtattcgaaa acttcgtcgg gtgacgatgc tgccatgact
     9601 tcgggggttg gaaacgcctc aaaaacacgc ggggaaatca ggtttacccg tttatcggtg
     9661 cattgtgccg acaggatgac ggcaacaagc agttgatagt tgttggcgta atgaagctcg
     9721 gtttcggaag attcggcatt ttccaggaac cagtccatca cataagcgaa gcgttgtttc
     9781 tttgtcattc taatcatctt tttatctcat ccgataacgg gataaatgtt gcgttgagat
     9841 cgggatcggc catatcggga tcgagtggat aaatggggcg gttgatccgc ttgtaatcca
     9901 gtcggagaag gtcctgatca accccgccgg gtgttaatgc cattaaccaa tcggcacgca
     9961 tgtcgtacaa ctcaggcacg aggtagccga ttttaaccac caggatatct gtttcacgcg
    10021 gatttaaccc cagtttggtg aagtcctttt cgtaatggta ggccatgcgt ctttcagtga
    10081 ctatcacaga ggtgttgccg tttcttacca ccacttcggt tttggcattg ggatgccctt
    10141 cgtggatggc cgtaatggtt ccggtgatcc ttaccggcgg tgcgaaccgg ttatctacgg
    10201 ccgctccggc tgtcgcatca atttttccgc caacaccaat ctccttggct ttcttcacaa
    10261 actcttcacc gggaatggag gcgtaaatca acgactttgc ccgcggcgac ttcaattccg
    10321 gccttttcag tatctcctgc agtgtccagg taacatctcc tgctcctcct gccgtggggt
    10381 tatcacccat atcgctgatg ataaacggtt ttttatcgct tgccagcgct ttttccaaag
    10441 cctcatccaa ataaacggta ggagccacaa aatcgaactc ttttctcacg ttccagaaat
    10501 gggcggccag tttttcggct gctttgccaa cagcctcctt gtcatctccg taagcaacca
    10561 ccacaccgtg atttctaggt tcatcggccc aagggtagga catccagatg gatgcgtcaa
    10621 tcaccttgtc tttttcaata acctccggaa tctgtgcata caggcttttc cccggctcga
    10681 tgcgggtgct ggtcttttcc ccggggagca ggatgggaac gggaatccac gctttatacg
    10741 caggctttcc cttgccgttt tcgaggcgat cgagaaggtt ggtaaccgcc ctttttttcg
    10801 actcaatggc atcttcgtgt ggtgccaggc ggtagcaggt aattaagtcg gtattctgtg
    10861 ccaaacgcgg cgaaacgctt ccgtgcaggt ccatcgaggt ggaaatcaac acatccgtgc
    10921 ccaccagttc gcgtatgcgg accaggaaat cgccctcggg atcatccaac ccttgaacgc
    10981 tcatagcacc gtgaacatca aaaaagaggg catccagggg cagttttgct tttaagctgt
    11041 ccagtgtttt tgacaccagc gattcgtagg cttcacggct tacaatgcca cccggcatgg
    11101 catgtccgcg caaggtcggc agccaaacgg cccggtcgat aatgcccgaa tcagcagcaa
    11161 agaacgggta ataactgaaa acactgtcgc cataccttgc ccggaacgca tcttcgtggg
    11221 aaacggcggg tgaaaacgtg ctcgactcaa tggcaagccc ggcaatagct attctcggaa
    11281 gcttcttttt atggttttga catcccacgg ctgaaatcag tatggccaaa aggagggaag
    11341 tagaaaaaag ttttttcatc tgtttgtaat tttttcggtt gggtttaaag catcaaaatt
    11401 agtaaaaaat caaatcagcg acaacaggta tcctgccaaa cagcctccga tcacaataaa
    11461 tgcactattg acttttcggt aacgaaagac cacagccata cttaatactg cgataacaac
    11521 cgttcgcatg tctgttatgg tctctttccc cattattaaa caaatggccg cgataatggc
    11581 aaccgaagca acgttcacgg catctagaaa cagggagaaa agcgttgatt ttcgcatttt
    11641 ctttacaaca ggattcaaca acgcaacgaa cacaaaagag ggtaaaaaga tgccgacagt
    11701 agagactacg gctccccaaa aaccgttgat ctggtaaccg ataaaggtaa ccgacgtaaa
    11761 aacggggccg ggagtaaact ggcctacggc aatggcatcg ataagttgtt gacgcgacat
    11821 gaacccggtt gtcaccagtt ccgtatccaa aaaagcgaac agcacgtaac cgctaccgta
    11881 taaaatagct ccgattttca ggaaaatcaa aaacaggttg acattggaaa ccgacaacag
    11941 gactgggact gttggtggca acaatgccag gggaacgatt ccgttgaatt ttattttctg
    12001 ccggttccgg attcgggcaa ccagcatcgc tagcaatcct gcaccaaaca tcaggtatat
    12061 ttcattgata ttcagcagag ataaaactag tactaggatg cctagaatag ccagttcaac
    12121 cgattttatc gacttttttg ccaacgggaa tacggctcca aggataacgg cgatgatggc
    12181 gggtttgatt ccgtatataa atggctgtac ctgtggtaac tggccgtaat ccttatacag
    12241 ccatgccaga aagccgacaa tcaacaccgc gggaaaaatg aagcaggctc cggcaatgat
    12301 caaccctctc cagcctcctt tttcctgtcc gatatggatg gccatctcag tactgttggg
    12361 gccgggaatc aggttggtgg cacccagaag gtcgagaaac tcctgttccg tgagccactt
    12421 ccttttttcg accacttcct gctgcatcat ggcaatatgc gcagccggtc cgccaaaacc
    12481 gataatgccc agcttaagaa aaagtgccgc gagttctttc agtatcggtt tgggcgtttc
    12541 tttcatcgtt tttacgttaa attcaacacg tgaagggtat ggcaagccac tagacaggcg
    12601 gtttccgttc tcaagcggtt ttcgcccaga ctcaccggtt caaagccgag actgacggct
    12661 ttttgaattt cttcttcact gaaatctcct tcgggaccga taagaatcag ggcatcttct
    12721 cccttttggt aggttttggc caacgctgtt ttaggggtag ggtggcagtg cgcgataaac
    12781 tttctgcccg taaatgggtt ctcaacaaac aagtcaaagg gattcatctc gtcaataact
    12841 ggcattacgg cttgttgcga ctgcttcatt gccgaaacga ctattttttc caacctttcg
    12901 gttttcaact ctttcctttc cgaaaagcgg caaagcaacg gtgtaaaccg gtctattccc
    12961 gattcggtgg ctttctcggc gaaccattcc atcctgtcga tgtttttggt aggcgcaaaa
    13021 gctatatgaa ggctgaaatt ccacgttttg ggttgagcaa cttccgcgtc catgctaacc
    13081 acgcaatgct tgggatgtgc ctgaatcagc gtacacgggt aaaagaagcc tttaccgtcg
    13141 gtaacggtca atttatcccc ttccttcatt cgaagcactt ttacgcaatg tagcgattct
    13201 tgttcgggca actgctgcaa gtgccggata ccgggcgaat aaaaaaccgt atcagccatt
    13261 ttctgcaccc tgccggcggt ttatctcatc gcgaagctta gtcgccaact cgtaatcttc
    13321 gtcttttatg gcttcttcca gccgtttttt caacgcgtcg atttttacct cctccatatt
    13381 ctccacgatc tcctccactt ccatgtcctt gttctgaaac tctgtggaaa gcacctcttt
    13441 ttcatcaaac acaatggcca tattctgcat gatggtttcc gtggtgaaaa tgggtgaatg
    13501 tgtcctcaat gcgatggcaa cggcatccga tgtccgggaa tcgatacgaa actcccgatt
    13561 gtcttgacgc aaaagcagct cggcaaaaaa agcaccgtcc tcaaatttat aaataaaaac
    13621 ttccagcaac tcgatactta aggattcgaa aatgttgtat atcagatcat gtgccagggg
    13681 gcggggaggg gtcatgtttt ccattaccag ggcaatggat tgcgcttcag gcgtaccaat
    13741 cacaatcggc agccggcgta tgccgttctc ttcagcaaat ataagcgcat acgctccggc
    13801 ttgcacctgg ctgaacgata tacccaatat ggagagttta actttattcg tcacaattat
    13861 tgctgttaat cggttataac ttcaaaaata caatttttaa acaagaaaaa caggaataag
    13921 tgcatttttt tgattttttg gaaatttatt ggggattatg aagaaaatta tttatctttg
    13981 cacccacttt acgacacggt ggttatagct cagttggtta gagcgacgga ttgtggttcc
    14041 gtaggtcgcg ggttcgaatc ccgttttcca cccgaataca ctggtaatag gtgttttctc
    14101 tcttagattt gttttaggta atcacgtatt ttacatacgt gatttttttt gccactatcc
    14161 gaaaagctct cctacttgtg ccttcctttt aacacccctt cgatgtcttc cagtttcaac
    14221 gcttttgtct tcaacaggat caggaagtga tagagcaggt cagccgcttc gtttttaaac
    14281 aggtcgatgt tgttatcctt agcttcgata acgagctcaa cggcttcttc acctactttt
    14341 tgcgccactt tatttactcc gctgttgaaa agcctgctcg tgtaggatcc ttcggtttta
    14401 gcttctatcc gttgttcaat cactttttcc agttcataga taaaaccttt cggtgtttct
    14461 tcggaaaaac aggttgtggc tccggtatgg caggtgggtc cctgaggaag ggctttgatc
    14521 agcaacgtgt cgttatcaca atcttcccgg atttcgttca cgataagata atttccggaa
    14581 gtttctcctt ttgtccataa ccggttctga ctgcggctaa aaaaagtaac ccggccttcc
    14641 gctttggttt tttcgagggc ttcttcattc atgtagccaa gcatcagcac ttgtaacgta
    14701 tgggtgttct ggatgattac cggaaccaat cctccggatt ttttgaaatt gatggtcata
    14761 atttttttac tttttaaatc ctcacttcaa tgttttgttg ttttaaaaac tgtttcaacc
    14821 ggggtatcgg tatttcaccg aaatggaaaa tgcttgccgc caatgccgca cttgcccttg
    14881 tctgttggaa gacatcagca aaatgttgca ttgttcccgc gccaccagag gcgacaacag
    14941 gaatattgac ggctgaggaa accgcggcgg tgatatcgag tgcgaaaccg tttttggtac
    15001 cgtcgttgtt catggatgta agcagaattt cccctgcacc gcgccgttcg ccctccagtg
    15061 cccagtcaac ggcattgagt ggagtaggag tcctgcctcc gtgaatataa accttccatt
    15121 caccgtcatc aagcttggtg tcaatcgcca gtacaacaca ctggctgcca aattttgcag
    15181 caatttccga aacaatctcc ggatgatcta cagccgcagt gttaatgctc accttgtcgg
    15241 ctcccgcttt gattattgcc tgcgcatctt ccaccgaatt tatgccaccg cccacggtga
    15301 acgggatgtt gatttcacgg gcaatctttt cgaccagtcc ggtcaggttc ttccgtttct
    15361 caacggtggc ggtaatatcg agaaacacca gttcatccgc accttcctgg acgtaacgtt
    15421 tggccagttc cacagcgtcg ccggcatcgc gaagccccac aaagttaatg cctttcaccg
    15481 tccggccatc cttgatatcg agacagggaa taattctttt tttaagcata taagtttata
    15541 ttttttgttg ttttaaaccc gttacaggaa acccgcaacg cgtaactcgt ccaggctaat
    15601 tctcccttcg taaagagctt tgccgataat ggctccctca caacccaact cctttacctt
    15661 tgtcagatca tccaccgatg atacgccacc actggcaatc agcttaacat cggtctcccg
    15721 gaggatttca gcgtaaagct cattggaagt gccttgcagc atgccgtctt tcgagatgtc
    15781 ggtacagatc acgtatgtaa cgcctttgcg ttcataaccg gcaataaaat ccacaac
//
LOCUS       DAOSDR010000015        15285 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrimonas sp. isolate AMR_MDS_1826
            NODE_1555_length_15285_cov_3.842810, whole genome shotgun sequence.
ACCESSION   DAOSDR010000015 DAOSDR010000000
VERSION     DAOSDR010000015.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863355
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrimonas sp. (wastewater metagenome)
  ORGANISM  Petrimonas sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Dysgonomonadaceae; Petrimonas.
REFERENCE   1  (bases 1 to 15285)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15285)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:10:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,720
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 35
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15285
                     /organism="Petrimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1555_length_15285_cov_3.842810"
                     /isolate="AMR_MDS_1826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2023866"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..766
                     /locus_tag="PLI30_01220"
     CDS             <1..766
                     /locus_tag="PLI30_01220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019537931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOI78205.1"
                     /translation="MVQEGKIGKIEKVNAYVGAPPTPYDLPEESVPADLNWPLWLGPS
                     EFVHYNSQLNPPISLDPEQKEQFWGAWRWYKELGGGFTTDWGAHMFDIAQWGLGMDNS
                     GPVEIIPAGYQDTKFLTYKYADGAVMTEEPFDEKQTKGVKFWGDKGWIEVSRGHYLAS
                     DDSLLPPKVEETEGAYETKVPHLENFINAVKTKTDPVVPVEIGHRSCTVCTLGNIAYD
                     MKRPIKWDPATEKFVDDPEADKNRLFNKTYTEGYIL"
     gene            921..2246
                     /locus_tag="PLI30_01225"
     CDS             921..2246
                     /locus_tag="PLI30_01225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019537931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOI78206.1"
                     /translation="MSNQLTRRKFIKSAAVGVVGVTVLPQFLASCNSNKKSGNEKIDD
                     GITRLGFIGLGQQGIGLLGAFLTIPGVEVVAGSDVYGIKRERFLKRLNDHYGSQQKTV
                     SVDVYENYKDLLARDDIDAVVIATPDHWHAFIAIDACKAKKHIYLEKPLTFTIKEGQE
                     LVKAVRENNVILGVGSQQRSDPNFQHAVKMVQEGKIGKVEKVNAYVGPGPHPYDLPEE
                     PVPADLNWKMWLGPLEYFHYNSRLNPPISLDPEQNESYWAEWRYNKGLGGGYTTDWGA
                     HNFDIAQWGIGMDKSGPVEIIPAGYAGTEFLTFKYDNGVVVTNEPFDEEKTLGIKFWG
                     DKGWIEVSRQFYHASDESLMPPKAETQGGRYETRVPHNQNFIDAVKSNIDPIVPVEIG
                     HSTCTTCNLGNIAIELKRPVKWDPQTQTFVDDPEATKYLTKTYSEGFSI"
     gene            complement(2307..4061)
                     /gene="aspS"
                     /locus_tag="PLI30_01230"
     CDS             complement(2307..4061)
                     /gene="aspS"
                     /locus_tag="PLI30_01230"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009276077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HOI78207.1"
                     /translation="MYRNRTCGELRIADINKEVTLSGWVHKIRKMGGMTFIDLRDRYG
                     VTQLSFNQEIDRELCEQANKLAREYVIQVTGKVAERSSKNQNIPTGDIEIVATAVTVL
                     NAAQTPPFTIETETDGGDDLRMKYRYLDLRRSIVRKNLELRHKMAFEARKYLDERDFL
                     EVETPVLIGSTPEGARDFIVPSRMNQGEFYALPQSPQLFKQLLMVSGFDRYFQIVKCF
                     RDEDLRADRQPEFTQIDCEMSFVEQEDVLNMFEGLTKHLFKTQKGIELPDFPRMSYAD
                     AMRQYGSDKPDIRFDMKFVEIKDLTTGRGFGVFDSSEYVGAICAQGCAAYTRKQLDEL
                     TDFVKRPQIGAKGLIYVRYNEDATLKSSVDKFFSESDLKLWAERCNAKPGDLLLVLSG
                     ETEKTQKQLSELRLEMGARLGLRDKNNYKCLWVVDFPLLEKDEELNRFFAKHHPFTSP
                     KPEDIALLDSDPGAVRANAYDLVINGVEVGGGSIRIHDSALQKKMFSVLGFTEERAQE
                     QFGFLMNAFKYGAPPHGGIAFGLDRLVSLFAGLDSIRDCIAFPKNNSGRDVMIDAPSR
                     IEKEQLDELGLIIVEKEK"
     gene            4206..4280
                     /locus_tag="PLI30_01235"
     tRNA            4206..4280
                     /locus_tag="PLI30_01235"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4241..4243,aa:Pro,seq:tgg)
     gene            4809..6539
                     /locus_tag="PLI30_01240"
     CDS             4809..6539
                     /locus_tag="PLI30_01240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007174452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HOI78208.1"
                     /translation="MRKMACLLTLFISISLFASERVPIWPKDKMPHRQDHQIAAMVDE
                     AGKKDFNADKNRVAYLEWFDAPPKEVRNGGCMILISGGSYESCCDVELIKLWNKTFTQ
                     LGFQCINFVYRTPRPVGLPIYQTAWEDGQRAVRKVRSEAKKRGFDPEKIGVISMSAGS
                     HLALLLATSSQTLAYEKVDPIDELPCNINWAIINAPAYGTTDAETGIPASRQGYGTDV
                     NLSNVFKFDERTCPMSLHHGGTDIYASQTSTLVYRQLRRMNIPAELHLYADKGHGAFG
                     LERGVEFMRQMGYMGKLAGEEELMKRYASDDSRTDYFKMNIWPEGKTPDIQPNQCTPY
                     LEWHTPKNLKIKAIQIIYSGGSYEGNDPDGFEVAPARRYLNEMGMTVVTLKYRTPRPT
                     TGLSKHTTAWQDLQRAIKIVRSEASKRGLDPERIGIMGSSAGGHLTLMGVTSSLHQSY
                     LPIDEIDKLRCNVQWGIGIYPAYALTDGSEQPNIDKGNEDSAVLVPELSFDLKTAPML
                     FVHGDADGWAAMNSVKTWEKMQQMGIQSELHTLATRDHCFQLKASPGTGSYTYLERIW
                     EFLSSKGFNK"
     gene            complement(6825..8129)
                     /locus_tag="PLI30_01245"
     CDS             complement(6825..8129)
                     /locus_tag="PLI30_01245"
                     /inference="COORDINATES: protein motif:HMM:NF025042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HOI78209.1"
                     /translation="MKKIAELIFFAGVNGVLPEKLMQSQVKLSGDLLQVAEKRFPLSR
                     FRHIYVLAAGKAAASMSRGVEKVLGDKITNGHVVTKYGHGMNLNYLTLTEAGHPIPDA
                     EGVKGTQKIVNIARKATEDDLVICLISGGASALMADFPEGVTLDDLKRTNELLTKCGA
                     NITEINTVRKHLSKIKGGQLARILFPATTVCLILSDVVGDRLDVIASGPTVGDSSSFA
                     DALTIIDKYSLQNRLPSSVLHYLLEGADGSIADTPKPDNPVFQNVHNYIIGSNGIALE
                     SSAKKAVELGFETHTVTDSIQEDVTETANFILKTIDNQKSTGKKPVCLLFGGEPTVKV
                     SGKGLGGRNQHLALYLATKICCKKNITILCAGTDGTDGPTDAAGAVVDYETATKAVEK
                     GIDPNHYLSNCDSYRFFQQVGGHIITGNTGTNVMDIIVAIVQ"
     gene            complement(8139..9467)
                     /locus_tag="PLI30_01250"
     CDS             complement(8139..9467)
                     /locus_tag="PLI30_01250"
                     /inference="COORDINATES: protein motif:HMM:NF014500.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntP family permease"
                     /protein_id="HOI78210.1"
                     /translation="MSILTISVLVLLSIVLIVILTSVTKLNAFASLFMVSLLLAVIAL
                     PGKDIVEILKQGFGNTVSSIGFLIIFGAIIAVVLEKTGGAASIANYILSKTGHRNAAS
                     AMGITGFIVGLPIFCDSGYIIMSGLAKSFSSKAKVALPFIAFVLATSLYSVHCLTPTH
                     PGALAASGILGANLGMLIVLGALFAIPAALAAFLWIKWQTRNDIYTETENSHHEIRSN
                     EQLPPIHLSLLPIVTPLLLIATGSLLTVLDISNVNFTLKGLAFLGQPIVSLMIGVLLS
                     LFLLKNRAIKNINIVLESAIEKAGPILIVTGAGGMFGLVIKETGVGAYAGEFLLQTGL
                     GLAVPFLIASILKTAQGSSTVAIITAASFVVPMLPALGLDSEPGKLLAMISMGAGSMM
                     VSHANDSYFWVVSRFSGIGSNTALKVYSSATVVMGIVTFLCVWLTSLFML"
     gene            complement(9607..10497)
                     /locus_tag="PLI30_01255"
     CDS             complement(9607..10497)
                     /locus_tag="PLI30_01255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI78211.1"
                     /translation="MQNAITKLTTVLIALITVLCFTSCGTSYYGAAENNYYDNYYDYN
                     PAWAPDYYFGTRYYYFPDIETYYDLGTRNFVYLNNGRWLFVPTLPPIYAAFNLNNAFI
                     VIVNRSVYTPWMHHHYYNSHYPRYYYIDYYDFSNIPYVRGYNENGRRAVYWREAERNH
                     AREWGDRNIREGRKFKYSTDDRRVQQEVTRRVAQRTAVSPEELRQIANTEEGRMTTTR
                     PRTARTSARQTTPNTTQRTSTSRSAETNYYGGEIGQPVKVQPQMRGNTSGRSSSTRSE
                     GTSTRSREKTTSGRSSDSRR"
     gene            complement(10571..12022)
                     /gene="sufB"
                     /locus_tag="PLI30_01260"
     CDS             complement(10571..12022)
                     /gene="sufB"
                     /locus_tag="PLI30_01260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005859390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HOI78212.1"
                     /translation="MQELDQDQILTDLTTQDYKYGFVTDVETYTIEKGLNEDVVRLIS
                     SKKNEPEWLLEFRLKAYRHWLTMKLPDWAQLNIPEIDFQEIIYYADPTQKKTPKSLEE
                     VDPELLKTFERLGIPLNEQKQLTGVAVDAVMDSVSIKTTFKEVLAEKGIIFSSFSEAV
                     KNHPDLVKKYLATVVPYKDNFYAALNSAVFSDGSFVYVPKGVRCPMELSTYFRINAQN
                     TGQFERTLIVADDNSYVSYLEGCTAPMRDENQLHAAVVEIIALENAEVKYSTVQNWYP
                     GDKNGKGGVYNFVTKRGICKGNNSKISWTQVETGSAITWKYPSCIMAGDNSVGEFYSV
                     AVTNNFQQADTGTKMIHLGKNTRSRIVSKGISAGSSQNSYRGLVKVSKKAINARNHSQ
                     CDSLLLTDHCGAHTFPYTDIQNPSAILEHEATTSKISEDQIFYCNQRGLGEEEAIGLI
                     VNGYVKEVVNKLPMEFAVEAQKLLQISLEGSVG"
     gene            12202..12771
                     /locus_tag="PLI30_01265"
     CDS             12202..12771
                     /locus_tag="PLI30_01265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019540841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HOI78213.1"
                     /translation="MKKTGLTFLVLFFMFGLMAQTRYLVDKAHSSVNFKVKHNGISFV
                     NGSFNSFEGFIEGDLNSLENANIEFTVDAESIDTRIEPRDKHLRSADFFDVEKYPKMS
                     FKSTDIEKTGDKKYKLNGLLTVKDVTKPVTFEVKYGGKIVGRDGKDIIGFTAKNSINR
                     LDYNVAYDPDSKAIAKDVDLVLYMEFVAN"
     gene            complement(12795..13664)
                     /locus_tag="PLI30_01270"
     CDS             complement(12795..13664)
                     /locus_tag="PLI30_01270"
                     /inference="COORDINATES: protein motif:HMM:NF025115.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3298 domain-containing protein"
                     /protein_id="HOI78214.1"
                     /translation="MRKNIFLPVAILFFLLLCTLFSCKKTAQISTKIVFDSIVIKDQI
                     PLLETNDTTLPFADVKISFTYPVKFGNKEDLARLQQIFIGTFFNSLHLDTLSPQEAVK
                     AYVSGYKEEYKSLSNTYYSEKSRLPKGEIPMWYWYYMYNTNKVMFQNDSLLSYAIEYS
                     DYTGGAHGSYRITYTNIDLGELVTVSEEDIFVSNYKKPLAEIIINRLMDQYNVSVPDS
                     LISLGFFNLDEVFPNNNFWLDHEGIHYSFNQYEIAPYSMGVINVDIPYGDLSAILKPE
                     NVTQKFFLNSGHR"
     gene            complement(13810..14892)
                     /locus_tag="PLI30_01275"
     CDS             complement(13810..14892)
                     /locus_tag="PLI30_01275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019540839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4831 family protein"
                     /protein_id="HOI78215.1"
                     /translation="MIKIKRLVLSALIVLLPAVSMQAQQTVRMNAIKANDYGVIYSLP
                     KTSLVVTLKVKKTVYNRGEFYQFAQRYLSIDPITESRTEFTLEDVMVTNRGVADKDNS
                     FMVIFRPNSIAPYVHLTQDGLISTINTDPESEKTPSFDVPEPSPAPLNPRRFLSEETL
                     MAGSTAKQAELVSKQIFELRRSRNDILIGEADNMPPDGEAYKVVMEQINNQEKALTEM
                     FSGSTQTEYFTKEIVVIPTEKDIDKRIIGRFSEKLGPVDADNLAGAPIYLTLRSKTQK
                     VETILSDKDKERLAKKLSEGVVYNVPGKAQLTLEFRNKTLKNMETDIVQFGTKDVLAK
                     KMFDNMKQPIKVVFYPDLGAIKQIIQ"
     gene            complement(14977..>15285)
                     /locus_tag="PLI30_01280"
     CDS             complement(14977..>15285)
                     /locus_tag="PLI30_01280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005857329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HOI78216.1"
                     /translation="KIRLRPSYFPFTEPSAEMDISCNLCGGEGCPFCKYTGWVEILGC
                     GMVDPNVLDNCGIDSRIYTGYALGMGIERITNLKYQVKDLRMFSENDLSFLKQFQAAH
                     "
BASE COUNT         4235 a         3151 c         3383 g         4516 t
ORIGIN      
        1 aatggtacaa gaaggaaaaa ttggaaaaat agaaaaagta aatgcttacg taggcgctcc
       61 cccaacaccg tatgatcttc ccgaagagtc cgtcccggcc gacttgaact ggccattgtg
      121 gttgggcccc tctgaatttg tacactacaa ctcgcaactt aacccgccta tctcgcttga
      181 tcccgagcaa aaagaacagt tctggggagc atggcgctgg tacaaagagc tagggggtgg
      241 attcactacc gactggggtg cccatatgtt tgatattgca caatggggat taggcatgga
      301 caatagtggt ccggtggaaa ttattcccgc aggatatcaa gatacaaaat ttctcacgta
      361 caaatatgct gatggcgctg taatgactga agaacctttc gacgaaaaac aaacaaaagg
      421 tgtgaagttt tggggtgata aaggttggat tgaagtatcg agggggcact acttagcttc
      481 ggatgacagt ttgttacctc ctaaagttga agaaacagaa ggtgcatatg aaacgaaagt
      541 cccgcatttg gaaaacttca tcaatgctgt aaaaacgaaa accgatccgg ttgtgccggt
      601 tgaaatcgga catcgcagtt gtacagtatg tacgctcgga aacatcgctt acgacatgaa
      661 gcgcccgata aaatgggatc ctgccacaga aaaatttgta gatgatccgg aagcggataa
      721 aaacaggttg tttaataaaa cctatactga aggttatatc ctttaaaaga agcaatttat
      781 ggcgtgaggg tgttctttaa ggaataccct catcattttt cctgctgatc catgtttaca
      841 taccattttg aataaaatcc agacaagtcg aaagactggt gtcgggtgtt aaattaactt
      901 atatttattt atctaaagtc atgagtaatc aattaacaag gagaaaattt attaaatcag
      961 cagccgttgg agtggttggt gtaacggtgc ttccccagtt tttggcttcc tgcaactcaa
     1021 acaaaaaatc gggaaatgag aagattgacg atggtatcac tcgtttaggg ttcatcggtt
     1081 taggtcagca gggtataggc ctgttgggtg catttctgac tattccagga gtggaggttg
     1141 tagcaggaag tgatgtatac ggtattaagc gggaacgttt tctaaaacgg ttgaacgatc
     1201 attatggatc gcagcaaaaa actgtttcag tggatgttta tgaaaattac aaagatcttc
     1261 tggctcgcga tgatattgat gccgttgtga ttgccacacc cgatcattgg cacgctttta
     1321 ttgccattga tgcttgtaaa gcaaagaaac acatttatct ggaaaaaccg ttaacattta
     1381 cgataaaaga gggtcaggaa ctggtcaagg ccgtacgcga aaataatgta attcttggtg
     1441 taggcagcca gcaacgttcg gatccgaatt ttcaacatgc tgtgaaaatg gttcaggaag
     1501 ggaagatcgg gaaagttgag aaagtgaatg cttatgtagg ccctggacca catccgtatg
     1561 atctgccgga agaacctgta cctgccgatt tgaattggaa aatgtggcta ggcccgttgg
     1621 agtattttca ttacaattcc cggttgaatc cacccatctc gcttgatccg gagcagaatg
     1681 aaagttattg ggctgaatgg cgttacaaca agggattagg cggaggatac actaccgact
     1741 ggggagcgca caacttcgat atcgctcaat ggggtatcgg gatggataag agcggtccag
     1801 ttgaaatcat tcccgccggt tacgcgggca cggaatttct cacgttcaaa tacgataatg
     1861 gagtagttgt gaccaacgag ccgtttgacg aggaaaaaac attgggaatt aagttctggg
     1921 gagataaagg ctggattgaa gtttcgagac aattctatca tgcttccgat gaaagtctga
     1981 tgcctcctaa ggcagaaact cagggaggaa gatatgaaac gagagttcca cataatcaga
     2041 actttatcga tgccgtaaag tcaaatattg atcccattgt cccggtagag attgggcaca
     2101 gcacgtgtac tacgtgcaat ctaggcaata ttgccattga actgaaacgt ccggttaaat
     2161 gggatccgca aactcaaaca tttgtagatg accccgaagc gacaaaatac ctcacgaaaa
     2221 catacagtga agggttttcg atctgatatt aaaaaagctg ccaaaatttt tttggcagct
     2281 tttttaatat tattgttcgc caaaacttat ttttcttttt ctacgataat caagcccagt
     2341 tcatccagct gctctttttc aatgcgcgag ggggcatcga tcataacgtc gcgtccggaa
     2401 ttgtttttcg ggaaagcgat gcagtcgcga atactatcga gcccggcaaa gagtgaaacc
     2461 agcctgtcca ggccgaaggc tatccctccg tgtggcggcg ccccgtactt gaaggcgttc
     2521 atcagaaatc cgaattgttc ctgggcgcgt tcttcggtaa aaccaagaac cgaaaacatt
     2581 ttcttttgca gcgcgctgtc atggatacgg atagatccgc caccaacttc cacgccattg
     2641 attactaaat cgtaagcgtt ggcgcgaaca gctcccggat ctgaatcaag cagtgcgata
     2701 tcttccggtt tgggggaggt aaagggatga tgcttggcga aaaaccggtt caactcttcg
     2761 tctttttcca gcaaaggaaa atctactacc caaaggcatt tgtaattgtt tttgtctcgt
     2821 aatcctaggc gggcacccat ttcgagacga agttcagaga gctgtttctg tgttttctcg
     2881 gtttctccac ttaacacaag aagcaggtct ccgggtttgg cgttgcaacg ttccgcccac
     2941 aatttcaaat cgctttcgga gaagaattta tctaccgatg attttaaggt tgcatcttcg
     3001 ttgtaacgaa catagattaa ccctttggct ccgatctgcg gtcgcttcac aaaatccgtc
     3061 aactcgtcga gttgtttgcg ggtgtatgca gcgcatccct gagcacaaat ggcaccgacg
     3121 tattccgatg agtcgaagac accaaaaccc cggccggttg tcaaatcctt tatttccaca
     3181 aacttcatgt cgaaacggat atcgggcttg tccgatccgt attgccgcat ggcatcggcg
     3241 tagctcatcc gcggaaaatc gggaagctca attcctttct gcgttttaaa aagatgtttc
     3301 gtcagtccct caaacatatt cagcacgtct tcctgctcaa cgaacgacat ttcacaatcg
     3361 atttgtgtaa attccggttg gcggtcggcg cgcaggtcct catcccgaaa gcatttcacg
     3421 atctggaaat aacgatcgaa tcccgaaacc atcagtagct gcttaaagag ctgcggcgat
     3481 tgtggcagcg cgtaaaactc tccctggttc atacgcgatg gtacaatgaa atcgcgtgca
     3541 ccttccggag tagaaccgat aagcacggga gtttctactt ccagaaagtc tctttcatcg
     3601 agatacttcc gtgcctcgaa cgccattttg tgacggagtt caaggttttt tctaactatg
     3661 ctgcgtcgta aatcgaggta gcggtatttc atgcgtaaat catcaccccc atcagtttct
     3721 gtttcgatgg tgaaaggagg cgtttgtgct gcgtttaaaa cagtcacagc cgttgctact
     3781 atttcaatat ctccggtagg tatgttttga ttcttgcttg atctttctgc tacttttccc
     3841 gtcacctgaa ttacatattc acgtgccagt ttatttgcct gttcgcaaag ttcgcggtct
     3901 atctcctggt taaacgataa ttgagtgact ccgtatcggt cgcgtaagtc aataaaagtc
     3961 atacctccca ttttacgaat tttgtgcacc cagcccgaaa gggtgacctc cttatttatg
     4021 tcggcgattc gtaattcgcc acaagtcctg tttctataca tatattttgc tcgtttgcca
     4081 tgtttgaatt gcaaatttac acatttctaa cgttgaaacc gcttatcgat taaaaaaagc
     4141 tgaaaagttt tgatattatc gggataaaat cgtacttttg cacccacgtt ttgaaaagaa
     4201 cgactcgggg tgtagcacag tccggttagt gtacctgctt tgggagcagg gggtcgcgag
     4261 ttcgaatccc gccaccccga cttaaaaatg agggagttac agttaatatt gtaactccct
     4321 taatttttga ttgaaataca gttgaaacat ttagtttcca gttttcgcta tattaactac
     4381 tatgaaatac atactttaat ctttcctgat ttttttaatg gaggtgtgat gaagtgctat
     4441 acttcacatt agctgcaaaa ctattctact ttctgttatt tattattaaa tgggttagtg
     4501 aattaatatc cataagttta catgcatcac tgattttttg gttatgttca aatctgcact
     4561 acttggagtt aagttaccag aaggataatt atgtatcaaa actatcgatg acgatgctta
     4621 ttcaatgaga ctgccataag aaaaaataaa tctctgctag cataaaaccg tagtggtatg
     4681 acaaaagtaa aaagtttttt ttgaatttct ttattttagc ttactccttt ttgaaactta
     4741 aagccttgta gcttcaacga atttacttac ttttgtatac gttactttat cttaaataat
     4801 attcagctat gagaaaaatg gcatgcttat taactttgtt tattagtata agtttatttg
     4861 ctagtgaaag agttcctatt tggccaaagg ataaaatgcc acataggcaa gatcatcaga
     4921 ttgcagccat ggtagacgag gctgggaaaa aagattttaa tgcagacaag aatcgggtag
     4981 cttacctgga atggtttgat gcccccccta aggaagtacg taatggagga tgtatgattc
     5041 ttatttctgg cggttcttac gagtcctgtt gtgatgtaga actcattaaa ctatggaata
     5101 aaacatttac tcaattaggg tttcaatgta taaactttgt gtataggacg cctaggcctg
     5161 tggggcttcc tatttatcaa actgcatggg aagatggaca acgtgccgta cgtaaagtac
     5221 ggagtgaagc taaaaaacgt ggatttgatc cagaaaaaat aggagttata tccatgtcag
     5281 caggttcaca tttagcttta ttgctagcga ctagttcaca aacattggct tatgaaaaag
     5341 tggacccaat tgacgaacta ccttgcaaca ttaattgggc gattattaat gctccagcct
     5401 atggtactac cgatgcagaa acaggtatac ctgctagccg acaaggatat ggaacggatg
     5461 tgaatttgag taatgttttt aaatttgatg aacgtacctg tccgatgagt cttcatcacg
     5521 gaggaactga tatttatgct tcacagacct cgacattggt ttaccgccaa ctcagacgaa
     5581 tgaatattcc tgccgaatta catctatatg cggataaggg acatggcgct tttggactag
     5641 aaagaggagt tgaatttatg cgccaaatgg gatatatggg taagctagct ggggaagaag
     5701 aattgatgaa gcgatatgct tccgatgata gtcgtaccga ttattttaag atgaatattt
     5761 ggccagaggg aaaaacacca gatattcaac cgaatcaatg cacaccttat ttagaatggc
     5821 atacaccaaa aaacctaaag ataaaagcga tacaaattat ctactcgggc ggaagttatg
     5881 aaggcaacga cccagatggt tttgaagtgg ctcctgcccg tcgctatcta aatgaaatgg
     5941 gaatgacagt agtaacctta aagtatcgta cacctagacc tactacaggg ttatctaaac
     6001 ataccacagc ttggcaagac ttgcagcgtg ccatcaaaat cgtacgaagc gaagcgtcta
     6061 aacgcgggct tgatccagaa cgaataggta ttatgggtag ttctgcaggt gggcatttaa
     6121 ctttgatggg ggttacttcg tctttgcatc agtcttattt gcctattgac gaaattgata
     6181 aacttcgttg taatgtacaa tggggaattg gtatttatcc tgcatatgcg ctaaccgatg
     6241 gctcggagca acccaatata gataaaggca atgaagatag tgccgttctc gttccagaac
     6301 tctctttcga tttaaaaacg gctcctatgc tatttgtaca cggagatgct gacggatggg
     6361 ctgctatgaa ctcagtaaag acttgggaaa agatgcaaca gatgggaatt caaagcgaac
     6421 ttcatacctt agctactcgt gaccactgtt tccagcttaa agcatctcca ggaacaggga
     6481 gctatacata cctggaacgt atttgggaat ttctttcctc aaaaggtttt aataaataat
     6541 agtttagtgt atctcttcaa actaaagttt atcagtggaa ttactaactt agccgggaca
     6601 cagggttaag cctaaaagct tatttttgtg tagtctgaga agtcaggtaa cacattttgt
     6661 ccggagtatc caatgtgctt gtatatattt gaatatattg aatcccgggg taatcataaa
     6721 gggagattct catcattggg gaacttaacc attgatgaat tttggaatca gttgattccc
     6781 tgtcgattca attttatggg cttgtcgtac aaatttaaac aaaattattg aacaattgcc
     6841 acaattatat ccatcacgtt tgtacctgta tttccggtga tgatatgccc tccaacttgt
     6901 tgaaaaaaac gataggagtc gcaattggag aggtaatggt tcgggtcgat acccttctct
     6961 acagccttgg tcgcggtttc gtaatccaca actgcccctg ctgcgtctgt aggcccgtct
     7021 gtaccgtcgg tgcccgcaca aagaatagtg atgttttttt tacaacagat ttttgttgct
     7081 aaatacaagg ccaggtgttg gttacgccca cctagtccct tccccgaaac ttttactgtg
     7141 ggctctccgc cgaatagcag gcaaacgggt tttttaccgg tggacttctg attatcgatt
     7201 gttttcagaa taaaattggc ggtttcggtg acatcctcct gtatactgtc ggttactgta
     7261 tgcgtttcaa agcctagttc caccgctttt tttgcggagc tttcgagagc gataccatta
     7321 ctaccgataa tgtagttgtg cacattttga aaaaccggat tgtccggttt gggagtatct
     7381 gcaattgaac cgtctgcacc ttcgagtaaa taatgcaaca cagacgaggg aagccggttt
     7441 tgtagcgaat acttatcaat aatagtcagt gcatccgcaa aactacttga atcacccaca
     7501 gttggcccag acgcaatcac gtccagccta tcaccgacca catccgataa tatcaggcac
     7561 accgttgttg ccggaaagag tatccgggcg agttgacctc ctttaatttt tgataaatgc
     7621 ttacgtaccg tattaatttc ggtaatattc gcgccgcatt tggtcagcaa ttcattggtg
     7681 cgttttaaat catcgagagt tacaccttcc gggaaatcgg ccattaaagc cgatgctcct
     7741 ccggatatca ggcagataac caggtcatct tcggttgctt ttcgggcgat attaactatt
     7801 ttttgagtac cttttacccc ctccgcatcg ggtataggat gaccagcttc ggtaagtgtc
     7861 aggtagttca ggttcattcc gtgtccatac ttggtgacaa cgtgaccgtt tgttattttg
     7921 tcgcctaaaa ctttttccac ccccctagac attgaggcgg ctgctttacc ggcagctaaa
     7981 acataaatgt gcctgaaacg ggataacggg aatctctttt ctgcgacttg caatagatct
     8041 ccggagagtt tcacctgtga ttgcatcagc ttctcaggca agacaccgtt cactccggca
     8101 aaaaatatca gttcggcaat ttttttcatc ttttccggtt acaacatgaa gagcgaagtc
     8161 agccacacgc aaagaaacgt tacaattccc atcacaacgg tggctgatga gtataccttc
     8221 agcgctgtat ttgagccgat tcccgagaac cgggaaacca cccagaagta agaatcattg
     8281 gcatgtgata ccatcatcga acctgcaccc atcgagatca ttgccaaaag ttttcctggt
     8341 tcagagtcaa gtcccaaagc gggtagcatt ggtacaacga aagatgcagc cgtgataatt
     8401 gccacggtag aagaaccttg cgccgttttt aaaattgatg ctatgagaaa cggaacggca
     8461 agtcccaatc ccgtttgcag caaaaactcg cccgcataag cgcccacccc ggtttccttg
     8521 atcaccagcc cgaacattcc tcctgcaccg gtaacgatca gtatcggtcc agctttttca
     8581 atagcagatt ctaggactat attgatattt tttattgccc tgttttttag tagaaacaac
     8641 gatagcaata ccccaatcat cagtgaaacg ataggttgtc cgagaaaagc caatcctttt
     8701 agtgtaaagt tgacattgga aatatccagt acggttagca acgagccggt tgctataagc
     8761 aaaagcggtg taacgatagg taacaaggaa aggtgaatcg gaggaagttg ttcattgctc
     8821 cggatttcat gatgagaatt ttcggtttct gtgtaaatgt cgtttcgggt ttgccattta
     8881 atccacaaaa aggcggccaa agctgcagga atagcaaaca gtgctcccaa gacgatcagc
     8941 atacccaggt tggcacccag aatcccggag gcggccaaag caccgggatg ggtgggagtg
     9001 aggcaatgaa ccgaatagag cgaagtcgcc agtacaaatg caataaaagg cagcgcgact
     9061 ttggctttgg aactgaatga ctttgccagt ccgctcataa tgatatatcc agaatcacaa
     9121 aaaatgggca atcctacaat gaatcccgtg attcccattg ccgaggcagc gttccggtgt
     9181 cctgtcttcg ataaaatata attggcgatg ctggctgcac caccggtttt ttccaatacc
     9241 acagcaatga ttgctccaaa aatgataaga aatccgatgg atgatacggt gttgccgaag
     9301 ccctgtttaa gaatttccac gatgtcttta cccggtagtg cgataaccgc aagcaataaa
     9361 gacaccatga acaacgatgc aaatgcgttg agctttgtga cggatgtaag gattacaatc
     9421 agtacgatgc tcagcagtac aagaacggaa atggtaagga tgctcatgtg tatttttagg
     9481 ttttttactg gctttccggt aagaattttt cagatttttt tgtttaaaca gctaataaca
     9541 gaggtaaaat aaatatattt tatgtgttta ttttaacgat cttgttttat tgcgaccgtg
     9601 aaatagttat cggcgggaat cagaactcct tcctgaagtt gttttctctc tgcttcgtgt
     9661 tgacgttcct tcactccttg ttgaagaact acgcccactt gtgttccctc gcatttgtgg
     9721 ctgaactttc accggttgtc cgatttctcc gccataataa ttggtttctg cagaacggct
     9781 tgtgctagta cgctgggtag tattgggtgt ggtctgtcga gcagaagttc gcgcagtgcg
     9841 tggtcgggtt gtagtcattc tcccttcttc agtatttgca atctgcctga gctcttcagg
     9901 cgatacggca gtacgttgtg ctacccggcg ggtaacttct tgctgcacac gccggtcgtc
     9961 ggttgagtat ttgaatttac gtccttcgcg aatgttcctg tcaccccatt cgcgtgcgtg
    10021 gtttcgttct gcttctcgcc aatatactgc cctgcgtccg ttttcgttgt atccccttac
    10081 gtacgggata ttgctgaagt cgtaataatc aatgtaataa tagcgtggat agtgcgaatt
    10141 gtaatagtga tgatgcatcc agggggtgta cacactgcgg ttgacgatta caataaatgc
    10201 attattcagg ttgaatgcag cgtaaatggg cggaagcgtt ggtacgaaaa gccaacgtcc
    10261 gttattgaga tatacgaaat tgcgtgtgcc cagatcgtag tacgtttcaa tgtcgggaaa
    10321 atagtaatag cgcgttccaa agtaataatc gggcgcccat gccggattgt agtcgtaata
    10381 attgtcgtag taattgttct ccgctgcccc gtaataggaa gttccacagc ttgtgaaaca
    10441 tagaaccgtg ataagtgcta tgagaacggt tgttaatttt gtaatagcat tttgcatttc
    10501 agtgtcagct ttaaatagtt atacagctaa ttaacttgtc ttcaatccac aacccataca
    10561 tacccggaga ttaacctaca ctgccttcca aactgatctg cagcagcttt tgcgcttcca
    10621 cggcaaactc cataggaagt ttatttacta cttctttcac atatccgttc acgattaatc
    10681 ctatggcttc ttcttctcct aaaccgcgct gattgcagta aaaaatctga tcttcactga
    10741 tttttgaagt agtggcttcg tgttccaaaa tcgctgaggg gttttggatg tcggtataag
    10801 gaaaagtgtg ggctccgcaa tgatcggtaa gtaacaaact atcgcattgt gagtggtttc
    10861 ttgcgttgat cgctttttta gaaaccttca ccaatccccg gtagctgttt tgactgcttc
    10921 ccgctgaaat tcctttggag acgatgcggc tgcgggtatt tttgcccagg tggatcattt
    10981 ttgttcctgt gtcggcctgt tggaagttgt tggtaacggc aactgaataa aactccccta
    11041 cggagttgtc tcctgccatg atacacgagg gatatttcca ggtaattgct gaaccagttt
    11101 caacctgtgt ccaggaaatc tttgaattat tgcctttgca gataccgcgt ttcgtaacga
    11161 agttataaac tccgccctta ccgtttttat cacccggata ccagttttgt accgtcgaat
    11221 attttacttc ggcattctca agggcaatga tctccaccac tgccgcgtgc agctgatttt
    11281 cgtcacgcat gggggccgtg catccttcca gataacttac gtatgaattg tcgtcagcaa
    11341 caataagcgt acgttcaaac tggcctgtgt tttgcgcatt tatacggaaa taggtagata
    11401 attccatcgg gcaacgaact cctttcggga catacacgaa agagccgtcg ctgaaaacgg
    11461 cagagtttaa tgctgcatag aagttgtcct tgtaaggaac cacggtagcc aggtattttt
    11521 tcaccaggtc ggggtggttt ttcacggctt cactgaagga gctgaatata attccttttt
    11581 cagcaaggac ctccttgaaa gtcgttttta tggagacact atccataacg gcatctacgg
    11641 ccacgccggt cagctgtttt tgttcgttga gcggaatacc taatcgttca aatgttttca
    11701 acaactccgg atctacttcc tccaacgatt ttggagtctt tttttgagtc ggatctgcgt
    11761 agtaaataat ttcctgaaaa tctatttccg ggatatttaa ctgcgcccag tcgggcagtt
    11821 tcattgtcag ccaatgacga tatgctttta accggaattc cagtaaccat tccggctcat
    11881 tttttttcga agaaattaac cggacaacgt cttcattcag ccctttttca attgtatatg
    11941 tttcaacgtc cgtgacgaat ccgtatttgt aatcttgtgt tgttaagtcg gttaatatct
    12001 gatcttgatc taattcctgc atgataaata ttgattatcg atcgctgtga tcggttctta
    12061 ataaagaaga gagcccaatt ccccctccat ggataaacaa actttgtatg gatttgttta
    12121 cgcaaaatta catcaatttc tgaacaaaac ctgtggtagg tgggttaaaa ggtggaatta
    12181 aattaaaaaa ggaataaaac gatgaaaaaa acaggtttaa catttttagt attgtttttt
    12241 atgttcggct taatggccca aacgagatat ttggtggata aagcacattc ttccgtcaat
    12301 tttaaagtga aacataacgg aatatctttt gtcaacggca gttttaattc tttcgaagga
    12361 tttatagaag gggatttaaa cagcctggag aatgccaata ttgagtttac ggttgatgcg
    12421 gaaagtattg atacccggat tgagccgcgt gataagcatt tgcgtagtgc ggattttttc
    12481 gatgtggaaa agtatccaaa aatgagtttt aaaagtacag acatcgagaa aaccggcgac
    12541 aagaaataca aattaaacgg tttgcttacc gtcaaagatg taacaaaacc cgttacgttt
    12601 gaggttaaat acggtggaaa aattgtgggc agagacggta aggatattat cggctttacg
    12661 gctaaaaaca gtatcaaccg gttggattat aacgtagctt acgatcctga ttccaaggcg
    12721 attgcaaaag atgtagatct tgttctttat atggagtttg tcgccaatta actgaatctt
    12781 tcaaaaaact cgggtcagcg atgacctgag tttaaaaaga atttttgtgt aacgttttcc
    12841 ggtttgagga tagccgacaa atccccgtag ggaatgtcaa cattaattac gcccattgag
    12901 tagggtgcaa tctcgtattg attaaatgag tagtgaatcc cttcatggtc cagccaaaag
    12961 ttgttgttcg gaaaaacttc gtccagatta aaaaaaccta agctgattaa ggaatcagga
    13021 acgctgacat tgtattgatc catcaatctg ttaattatga tttctgcgag tggcttttta
    13081 taattggaca caaagatgtc ctcttccgaa acagtaacca attcacccag atcgatgttc
    13141 gtataagtaa ttctgtacga gccgtgtgca ccaccggtat aatcactata ttctatagcg
    13201 taactcagca gtgaatcgtt ttgaaacatc actttattgg tgttgtacat ataataccag
    13261 taccacatag gtatctcacc ttttggaagt cggctctttt cggaataata ggtgtttgac
    13321 agcgacttat attcctcttt gtaaccggaa acatatgcct taacagcttc ttgcggagaa
    13381 agggtgtcga ggtggaggct gttgaaaaat gtaccgataa agatttgttg caaacgggca
    13441 aggtcttcct tgttgccaaa ctttaccgga taagtaaagc ttattttaac atcggcaaaa
    13501 ggcaatgttg tgtcgttcgt ttcgagtagg ggtatctggt ctttaatcac gatactgtca
    13561 aaaactattt ttgtggaaat ttgtgcggtc tttttgcagg aaaacaatgt gcacaaaagt
    13621 aagaaaaaca aaatagcaac gggaagaaaa atattttttc tcatatctaa attatttgtt
    13681 gggactgcca ataaaaattc ttaccctgca gtctctggtt ttccaccttt atatccatgc
    13741 gtcaggtctg aacaaaatac ctttttccgg caatgtcctt gacgccggca ggatgaatag
    13801 ggtgtctgat tactgaataa tctgttttat ggcacccaga tcgggataga aaactacctt
    13861 aatgggttgt ttcatattgt cgaacatttt tttggcaagt acatcctttg tgccaaactg
    13921 aacgatatct gtttccatat ttttcaacgt tttgttcctg aactccagtg tgagttgcgc
    13981 ttttcctgga acattataca ctactccttc ggagagtttt tttgcgaggc gttctttatc
    14041 tttgtcggaa agaatggtct ctactttctg tgttttactt ctcagtgtca ggtaaatggg
    14101 agccccggca agattatcag catccacagg accaagtttt tcggaaaacc tgccaataat
    14161 ccttttatca atatcttttt ctgtggggat aaccacaatt tctttggtga aatattcagt
    14221 ttgtgtactt cccgaaaaca tttcagtcaa tgctttttcc tgattgttta tttgctccat
    14281 aactactttg taggcttcac cgtcgggcgg catattatcg gcttcgccga tcaggatatc
    14341 gtttcggctt cgccgtagct caaaaatctg tttcgagacc aattctgcct gcttggctgt
    14401 tgaacccgcc atcagcgttt cttccgacaa aaaccttctc gggtttagcg gagcgggaga
    14461 tggctcagga acgtcgaaag aaggtgtttt ttctgattca gggtctgtgt ttatggtaga
    14521 aatcagtccg tcttgtgtga gatgaacgta aggcgcgatt gagttaggcc tgaagattac
    14581 cataaacgaa ttatccttgt cggcgactcc tcggttcgtt accattacat cttcaagtgt
    14641 aaattcggtt ctgctttcgg tgataggatc aatactcaag tatcgttgtg cgaactgata
    14701 aaactcaccg cgattgtaaa ccgttttttt tactttcagc gttaccacca gagatgtttt
    14761 cggcaatgaa tagattaccc cgtagtcgtt ggctttgatg gcattcattc tcacggtttg
    14821 ctgagcctgc atgctaactg cagggagtaa aacaattaaa gcggacaata caagacgttt
    14881 tatttttatc atatcatttg ttgttttatg atattcaatt gtttagttga atagtttccc
    14941 gacactacga atagcggcca tattgaattt taaagtctaa tgtgctgcct gaaattgctt
    15001 aaggaaactc aaatcgtttt ccgaaaacat ccgcaggtct tttacctggt atttcaggtt
    15061 tgtaatacgc tcaataccca ttccaagagc gtatccggtg tagatcctac tatctattcc
    15121 gcaattatcc aatacattgg ggtcaaccat gccgcatccc aagatttcca cccaaccggt
    15181 atatttgcag aatgggcaac cttcacctcc acataggtta caggagatat ccatttctgc
    15241 actgggttct gtaaacggga aataagaagg acgaagccgg atttt
//
LOCUS       DAOSDR010000016        15230 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrimonas sp. isolate AMR_MDS_1826
            NODE_1560_length_15230_cov_4.059044, whole genome shotgun sequence.
ACCESSION   DAOSDR010000016 DAOSDR010000000
VERSION     DAOSDR010000016.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863355
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrimonas sp. (wastewater metagenome)
  ORGANISM  Petrimonas sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Dysgonomonadaceae; Petrimonas.
REFERENCE   1  (bases 1 to 15230)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15230)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:10:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,720
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 35
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15230
                     /organism="Petrimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1560_length_15230_cov_4.059044"
                     /isolate="AMR_MDS_1826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2023866"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..242
                     /locus_tag="PLI30_01285"
     CDS             <1..242
                     /locus_tag="PLI30_01285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI78217.1"
                     /translation="TQVSHHNGGDWLLVIRVNGSIQKEVTIGKDTVDENGWLDVEFDL
                     TPFAGNKNVKIDLENKANGWAWEAAYWSDIRIENL"
     gene            406..2145
                     /locus_tag="PLI30_01290"
     CDS             406..2145
                     /locus_tag="PLI30_01290"
                     /inference="COORDINATES: protein motif:HMM:NF019556.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 127 protein"
                     /protein_id="HOI78218.1"
                     /translation="MENDIQNSLVNWNKGVLPYATFVDLFRVGRTQFAVGEMWGKATR
                     SASLFYRYTQDPELKTILDETVKDLLSTERPNGSISCSPIEKQPESATGDIWERKYVM
                     LGLMDYYDWVNHDPAVLQSLIRQADCVIDQVGPAPKSPINQMGWSSEKIESSTILEPI
                     MRLYQMTGYQRYLDFATYIIEAGGAKNHDLFENAYNNVEPYKMAGTYPKAYEITSLFE
                     GLAKYYRVTGKPEVKRMLDNYYNNVRTKEMTIIGNGGADQPYFPKVNGEAWTNTALEQ
                     THPNIKRMMETCVGVTWMKYCSQLMRLTGESSPMDQIERYIYNGLLGAMKPTGDGFSY
                     ANLLNGKKVNASGWGRSFNGLLVTCCNLSGPVGLSYIPLIAVTNSERGPVINLYNAAD
                     VNSTTPKGQALGLKIETDYPHSGKVKIHVNPEKAERFAVQIRIPEWSKKNKVKVNGKS
                     LKVESGQYAIIDREWKNNDRIEIEFEMIARVIDGPQGSAPGSENFQAVTWGPIVLARD
                     ENIDPTFNQPTKIKENKRGVVKVTKTTPTRSDARMEFIVPTTKGNIRMVDYASVNCWE
                     DSHVCTWLPLIKE"
     gene            2234..3241
                     /locus_tag="PLI30_01295"
     CDS             2234..3241
                     /locus_tag="PLI30_01295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016272600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015144 - carbohydrate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034219 - carbohydrate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GRP family sugar transporter"
                     /protein_id="HOI78219.1"
                     /translation="MFIVGNYLLAVILCVVTMICWGSWGNTQKLAAKTWRYELFYWDY
                     VIGIVLLSIIFGFTLGSIGEQGRGFIDDVTQVNPKSFWSAFTGGIIFNASNILLSASI
                     SLAGMSVAFPVGVGLALVLGVFINYFGAPKGDPVILFLGVLLVVTAIIMNGIASSRMS
                     KGTEEQKTRKKGIIIAIIAGFLMSFFYRFVASAMDLTNLENPTEGMLTPYGAFFIFSI
                     GILASNFILNTLVMKKPFVGNPVNYSQYFKGNLKTHLVGVLGGVIWGVGTAFSYIAAG
                     KAGAAISYGLGQGATLVAALWGVFIWKEFKNSSKATNKLLIWMFILFILGIISIIYAG
                     Q"
     gene            3515..5392
                     /locus_tag="PLI30_01300"
     CDS             3515..5392
                     /locus_tag="PLI30_01300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009134424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 127 protein"
                     /protein_id="HOI78220.1"
                     /translation="MRLKKIFLATIAVIISVTCTGCRQNSHSSNKISTRKITPVDFSH
                     VKINDNFWSPRLKNHVLHTLPVCIDQIENKTGRIRNFENAARRSGEHSGIFYDDSDVY
                     KALEGMAYSLINNPNPELERKADEWIDKFAAAQEPDGYINTFYTLTGRDKRWTNMDKH
                     EMYCAGHMMEAAVAYYKATGKRKLLDVSIRMADHMMDRFGPGKRHWVPGHEEIELALV
                     KLYEVTNREKYLDFAYWLLEERGHGHGSKGTEGTWNPMYYQDEMPVRELSTINGHAVR
                     AMYLFCGMADVAALKNEAGYVDALNRLWDDVVLTKMYLTGGIGSSRHNEGFTEPYDLP
                     NHEAYCETCASVGMVYWNSRMHQLTGDSKYMDVLERSMYNGALAGVSLKGDLFFYVNP
                     LASYGDHHRKEWYGTACCPSQISRFLPSIGNYIYGTSERAVWVNLYIGNKADVNTGKE
                     TMTLVQETSYPWDGNVTLTVESLKKSVRKEFRLRIPGWCKNYTVAVNGESITDPLIEK
                     GYLVIDRKWKSGDKVTLALDMPAETVQADPRVKENSGKRAVQRGPIVYCAEETDNPSF
                     DELTLSPPARFKETFNPTLLNGVTTIDAVSGDDTIRFIPYYAWDNREAGQMKVWMNYN
                     E"
     gene            5403..7442
                     /locus_tag="PLI30_01305"
     CDS             5403..7442
                     /locus_tag="PLI30_01305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009860424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 127 protein"
                     /protein_id="HOI78221.1"
                     /translation="MRHLIAILISVFLIPGTHSQGSNSGQPPNYPGNRAPLTGKTYIE
                     LPVGAIRPQGWLKEQLNRMRTGMTGHLDSIYPQVAGQRNGWLGGDGDVWERGPYWIDG
                     LLPLAYIMNDAELKKKVQPWIEWTLASQKPNGYFGPDTDREPEEGLQRNNAHDWWPKM
                     VMLKVMQQYYSATGDKRIIPFMTNYFKYQLKELPNTPLGNWTFWGEQRGGDNLMMAYW
                     LYNITGDPFLLELGELIHEQTFNWTDVFLRQDHLSRQHSLHCVNLGQGFKEPAIYFQQ
                     NQNPELLEAVKKAVRDMRNTIGLPIGLWAGDELLRFGNPVQGSELCTAVEMMYSLESI
                     LQVTGDVQWADHLEKIAYNALPTQTTDDFDARQYYQQVNQVKVSRDMRNFVTPHHDTD
                     NLFGELTGYPCCTSNMHQGWPKFTQNLWYATNDNGIAALVYAPSEVSARVGNGQLITV
                     KESTQYPFEETIAFTFDFPDREEEEIEFPFHLRIPRWCKNPVITINGEGVTAETRQGE
                     IARIDRAWKSNDKVVLRLPMEVSISRWYDGGAAVERGPLLYALKMNENWQKKTTEPHE
                     TKRYGKWYYQVTSDSPWNYALPIRNLSKENVGKAFVVETKNRASDYPWTLQDAPVTIR
                     TTGLRLPGWKEYNGSAGPVPYFTQQGEDVGGEENIELIPYGCSTLRIAVFPVRNK"
     gene            7499..8530
                     /locus_tag="PLI30_01310"
     CDS             7499..8530
                     /locus_tag="PLI30_01310"
                     /inference="COORDINATES: protein motif:HMM:NF012335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HOI78222.1"
                     /translation="MKALVLDKYMELNYRDFPDPHMEADEVLIRVQACGICGSDIHGM
                     DGSTGRRIPPLVMGHEAAGIITEVGTAVTGWKAGDRVTFDSTIYPLDDWFTRQGHYNL
                     SNNRKVLGVSPGNYTKHGAFAELVSVPAHILYKIPDNVSFEQAAMVEPVAVALHAVNV
                     SGIQLGQNAVVIGTGTIGLFVVKLLQTAGVYPIIAIDMDEKKLELSKAFGATHTFLSS
                     DKTVSEKIQQLTKGRNADVAFEAVGVSETVNLCINSLRKGGKAILIGNLMPEVTVPLQ
                     KIVTTELSLLGSCAINGEYETVLELMASGKIDVGGMISAVAPLSEGAEWFNRLYRKEA
                     GINKVILIP"
     gene            8543..9673
                     /locus_tag="PLI30_01315"
     CDS             8543..9673
                     /locus_tag="PLI30_01315"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOI78223.1"
                     /translation="MRNSVYNIAILGCGKVAHLHASAIQNLPNARLAGVWSRTQQTAN
                     GFACQYKVPAYTDVAELIKKEKIDLAVVCTPHPFHLEPAQQAALQGANILVEKPLAST
                     LEDADKIISTCKKAGVKLGVVSQRRWYEPVLRVKKAIEAGKIGKPVLGTVNMLGWRDK
                     SYYDADKWRGNWKTEGGGVLVNQAPHQLDILLWFMGEVEEVYGIWRNLNHPYIEVEDT
                     ALAVIKFRNGGIGNVIVSNSQKPGIYGKVHVHGENGASAGVQTDGGAMFIAGMKGILE
                     PPVNDLWTIPGEEELAEKWKMEDVRHFSSIDPMTHYMERQIEDFLFALDENRDPLVTG
                     EDGRRTVELFTAIYRSTRDNLPIKLPLYPENKNDMDGRSGLL"
     gene            9808..10719
                     /locus_tag="PLI30_01320"
     CDS             9808..10719
                     /locus_tag="PLI30_01320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019538106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HOI78224.1"
                     /translation="MNRKDFIKSAAVIAGAAAVTPFASASVSQSKPKKKIALKKSLGL
                     GMIKEELSLTDKFKLVKELGFDGVELNSPVDFNMSEILDAKSKSGIELPTVVNKDHWS
                     SPLSDPDPAVRKKCIDSVIKSLQEVKEMGGDTVLVVPGVVNEKVSYEQAYITSQQSIR
                     ELIPYAEKTGMQIAVENVWNNFLISPVEAKRFIDEINHPLVGWYFDIGNVLRYGWPEH
                     WIKTLNSRIMKLHIKEFSRELMNSKGLWEGFNVDLLKGDNNWPVVMKAVREINHQGGW
                     LTAEVNGGDRNHLKQISAQMDEIISYL"
     gene            10774..12126
                     /locus_tag="PLI30_01325"
     CDS             10774..12126
                     /locus_tag="PLI30_01325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009133622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOI78225.1"
                     /translation="MRNKDSLSRRSFLKSSVVAGTVGMMGGIPMLSSCSTDKSKKGEL
                     TLPKLLEQAPDGKVIKAGLIGCGGRGTGAAMDFLNAGPNLQIVALGDVFQDKLDSCRE
                     ILKKERNVEIPDENCFLGFDSYEKVIDSGVDMVLLCTPPHFRPAHVEAAVNAQKHIFM
                     EKPIAVDPFGVRKVLAAVKRAKTLNLNIVSGTIRRSQKDYMETRRRVLNGEIGDIVGA
                     NIIRNGGALWFRTRKPEWTDMEYMLRNWVNFCWLSGDHITEQFIHEVDVMNWYLGKNP
                     VKATGWGGRQRRVTGDQYDFFSIEYLYDNGMRTHCAARQIDGCTNEKVEQINCTNGYA
                     DASGKLYDLNGNIIWEYPHPEETDTQSEWKVTNPFVQEHINLVTAIRSGNTINDGEDQ
                     AYSSLVTIMGRMAAYTGKDITWDEVLNADLYLGPKTYVMGPVENIPEIIPVAGVPGKE
                     "
     gene            12137..13489
                     /locus_tag="PLI30_01330"
     CDS             12137..13489
                     /locus_tag="PLI30_01330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009133794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOI78226.1"
                     /translation="MKHFNILFLALIFTVSGFAQPSGQTQNTEPPREKGQKSVLQLAV
                     DPIPTVRVGFIGLGNRGTGAVGRYTFIEGVEIKALCDLDADKVKRSQNTLAKRGLKPA
                     DEYTGENAWKELCRRKDIDLIYICTDWLHHTPMAVYAMQQGKHVAIEVPAATSIEECW
                     QLVDTAEKTRKHCMMLENCCYDFFELNTLNMAQQGLFGNIVHGEGAYIHDLRESNFRS
                     GAYWDMWRLKFNTAHTGNPYPTHGLGPICQVMNIHRGDKMNYLVSVSSNQFGMTEYAK
                     EKFGENSAQAKQEYKLGDMNTTVIKTEKGRTIMIQHDVTSPRPYSRLHTISGTKGFFQ
                     KYPTGLIALEPNAHSFLTETQIDSLMTQYEHPFIKEIGEFAKKVGGHGGMDFIMDYRL
                     IYCLRNGLPLDQDVYDAAEWSCLVELSEQSVLNGGMPVKIPDFTRGDWNKLSGLKFAH
                     "
     gene            13767..14480
                     /locus_tag="PLI30_01335"
     CDS             13767..14480
                     /locus_tag="PLI30_01335"
                     /inference="COORDINATES: protein motif:HMM:NF018178.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1080 domain-containing protein"
                     /protein_id="HOI78227.1"
                     /translation="MKKQVILISIILSVFLLKTSRTSSQELVATFGKSIHMKDVLSGK
                     LKNAKPVKWYQVNTEADSWRVSGETLKCTGLPIGVIRSEKEYENFIMHIEWSHRAPGG
                     NSGTFVWSKAQPGENRLPDGVEVQMLDLEWIRLNTRDGVEPPIAYVHGELFGVGGVEI
                     IPENPRGKRSKSIENRVKGTKEWNIYEVVCVDGNIKLSVNGKFVNGITNSSQKKGYIC
                     LEAEGSEIHFRNIQIIELD"
     gene            14599..>15230
                     /locus_tag="PLI30_01340"
     CDS             14599..>15230
                     /locus_tag="PLI30_01340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylxylan esterase"
                     /protein_id="HOI78228.1"
                     /translation="MKYKLTLFFFIYFLGYYGYSQDFSPSSFANNQGEGKWMVYKDNF
                     RALYRILYNEAEDLLGKRSEYVSTIQTKADWIDYQKELKQKYRASLMKFKKTPLNTRI
                     TGQLEREKFTVEKIIFESHPGFYVTGCLFIPKKRQHPAPAIIYCSGHSDNGFRSEGYQ
                     RSIINFVEKGFIVFAYDPIGQGERLQYLDETTGKSQIGGPTTEHSYAGIQT"
BASE COUNT         4592 a         3252 c         3744 g         3642 t
ORIGIN      
        1 agactcaggt gtctcaccat aacggaggcg attggctact ggttattcgg gtcaacggtt
       61 ctatacaaaa agaggtgacc atcgggaagg ataccgtaga tgaaaacgga tggttagatg
      121 ttgaatttga cctaactccc tttgcaggaa ataaaaatgt caaaatagac cttgagaata
      181 aagcaaacgg gtgggcatgg gaggccgcat actggagtga tatacgcata gagaatctat
      241 agtctaccat ccactctgtt tattataaat tcaatacaat ttaaaaaagt tatgctacga
      301 aacactatat ttattttact ttttagctta tcagcaacac tgttttcgca ggtaaaagac
      361 caacagtttc cattagcccc aaattccata aaactacatg ggaatttgga aaacgacata
      421 caaaattccc tggtcaattg gaataagggt gtactccctt acgccacttt cgttgatttg
      481 tttcgcgtgg gaaggaccca gttcgcagtg ggtgagatgt ggggtaaggc aacccggtcg
      541 gcaagcttgt tctatcgtta cacccaagat cccgaactga aaacaatatt ggatgaaacc
      601 gttaaggatt tgctctctac cgaacgcccg aatggaagta tcagttgttc gccaatagaa
      661 aagcaacctg aaagcgccac aggagatata tgggagcgga aatacgttat gcttggactg
      721 atggattatt acgattgggt aaaccacgat ccagctgttc ttcaaagcct tattcgtcag
      781 gcagactgtg ttattgacca agttggtccg gctccaaaat caccaattaa tcaaatggga
      841 tggagctccg agaaaattga gtcgagcacg atacttgagc cgattatgag actctaccaa
      901 atgacgggct accaaaggta tctcgatttt gcaacctata ttatcgaagc gggaggagcg
      961 aaaaatcatg acttgttcga aaatgcatac aacaacgttg agccctacaa gatggcagga
     1021 acctatccca aagcatacga gataacatct ctgtttgagg ggttggcaaa gtattaccgc
     1081 gtaacgggga aacctgaagt gaagcgcatg ttagacaact actacaataa tgtgcgtacg
     1141 aaagagatga ccattatcgg caatggagga gccgatcagc cttattttcc gaaagtaaat
     1201 ggagaggcat ggactaacac cgctcttgaa caaacccacc cgaacatcaa gcgaatgatg
     1261 gagacctgcg tgggtgttac atggatgaag tactgcagcc aattgatgcg gttaaccggg
     1321 gagtcttctc cgatggacca aatcgaaaga tatatatata acgggttgct gggtgccatg
     1381 aagcctaccg gggatggatt tagttatgct aacctgctca acgggaaaaa agtaaatgcc
     1441 agtggctggg gacgtagctt caatggtttg cttgttactt gctgtaacct gagtggtccg
     1501 gtaggtttgt cgtatatccc gcttatcgct gtgaccaact ccgagcgcgg accggttatc
     1561 aacctctaca atgcggcgga tgtaaattcc actacgccga aaggacaagc tttgggattg
     1621 aagattgaga ccgattatcc tcattcggga aaagtgaaaa tccatgtgaa ccccgaaaaa
     1681 gccgagaggt tcgcggtgca gataagaata cctgaatgga gtaagaaaaa taaggtgaaa
     1741 gtgaacggta aaagcctaaa ggtggaatcg ggacagtatg cgattattga tcgtgaatgg
     1801 aaaaataacg atcgaatcga aatagagttt gaaatgattg ccagagtgat agacggacct
     1861 caaggaagcg ctccgggaag cgaaaacttc caggcggtta cttggggacc cattgtattg
     1921 gcgcgcgatg aaaacatcga tcccacattc aatcagccaa caaaaattaa agagaacaag
     1981 cgtggcgtgg tgaaagtgac taaaacaaca cccacccgtt ccgatgcccg catggagttt
     2041 atcgtaccta ccacgaaagg caacatacgt atggtagatt atgcctctgt gaactgttgg
     2101 gaggattcgc acgtatgcac atggttgccc ctgataaaag aataacaatg aaagaaatta
     2161 atcaacatcc ctacaaaacg tggagaggtg tatttgactt gatgaacaaa taactaattt
     2221 aatttcaaag gatatgttta tagtaggtaa ttatttatta gccgtaattt tatgtgtcgt
     2281 aaccatgata tgctggggct cttggggaaa cacacaaaag ttagccgcta aaacttggcg
     2341 gtacgagttg ttttattggg actacgtaat cggaatcgtg ctcctttcaa tcatctttgg
     2401 ttttaccctc ggaagcatag gtgaacaagg acgagggttt attgatgatg ttacgcaagt
     2461 aaatccgaaa agtttctgga gtgcatttac aggaggaatt atcttcaatg cttccaacat
     2521 attactttcc gcttctatct ctttggccgg aatgtctgtt gctttcccgg tgggtgtagg
     2581 cctggcactt gttttgggcg tttttatcaa ctacttcggt gcaccaaaag gcgatcccgt
     2641 cattttattc ctaggtgttc tcctggttgt cactgcgatc ataatgaacg gcattgcctc
     2701 aagtagaatg agcaagggaa ccgaggaaca aaagacaagg aaaaaaggaa ttattatcgc
     2761 aatcattgct ggattcctga tgtccttttt ttatagattc gtggcttctg caatggattt
     2821 gacaaattta gaaaacccta ccgaaggcat gctgactcct tatggcgcgt tttttatatt
     2881 ctcgataggg atattagcaa gcaatttcat tctcaatacc ctggtaatga aaaaaccatt
     2941 tgtgggaaat cctgtcaatt actcccagta ttttaaaggc aatttaaaaa cgcatttggt
     3001 aggagtattg ggaggcgtta tttggggagt tggtacggct tttagttaca tcgcagcagg
     3061 aaaagccggt gcagccatat cctatggttt aggacaaggt gctactcttg tggcagcact
     3121 ttggggtgtg tttatctgga aagagtttaa aaactcctcg aaagcaacca acaagctgtt
     3181 aatatggatg tttatcctgt tcatcttagg cattatttcc atcatttatg ccggacaata
     3241 agtctgcaaa gggatactgt aagaaactgt tttgattttt tcatgtcctg ttttatgcct
     3301 attttgggat ggatttggat tttcagttcg cacttgatag cgggctattt tgaagaaata
     3361 taaaattcaa aacagtttct aaattttcat tctttcttaa aaagggtcgt taactgtatt
     3421 gttgacaacc ctttttgtcg ttttttcagt tttcaaacga aaaataatta tctttgatac
     3481 cggaaaatat cctcagccat aatatctatt aacaatgaga ctcaaaaaaa tatttctggc
     3541 aacaattgct gttataataa gcgtgacatg cacaggatgc cgacaaaatt cacattcaag
     3601 caataagata tcgacaagga aaataactcc ggtcgatttc tcacacgtaa agatcaacga
     3661 caatttctgg tcgccaagat tgaaaaatca cgttttacat acacttcccg tatgtatcga
     3721 tcaaatcgag aataaaaccg gacggatacg gaacttcgaa aatgccgcca ggcggtcagg
     3781 cgaacattcg ggcatttttt acgatgattc cgacgtttac aaggcacttg aggggatggc
     3841 ttacagcctt ataaacaacc ccaaccccga gctggaaagg aaagccgatg agtggatcga
     3901 caagtttgcg gccgcccagg agccggacgg atacatcaat acattttata cgctgactgg
     3961 aagggataaa cgctggacaa acatggacaa gcacgaaatg tattgcgccg ggcacatgat
     4021 ggaagcggcc gtggcctatt acaaagccac cggcaaacgc aaactgctcg acgtctccat
     4081 acgaatggcc gatcacatga tggaccggtt cgggcccggt aaacgccact gggtcccggg
     4141 gcacgaggag attgagctcg cgctggtaaa actatacgag gttaccaacc gggagaagta
     4201 cctcgatttt gcgtattggc tgcttgaaga acgcggacac gggcacggtt ccaaggggac
     4261 ggaagggaca tggaacccca tgtactatca ggatgagatg ccggtcaggg aattatcaac
     4321 cataaacggg catgccgtcc gggccatgta cctgttctgc ggcatggccg acgtggctgc
     4381 cttgaagaac gaagccggct atgtcgacgc gttgaaccgg ttgtgggacg atgtggtatt
     4441 gaccaaaatg tacctgaccg gagggatcgg gtcatcacgc cacaatgaag gattcacgga
     4501 gccttacgac ctgcccaacc atgaagcgta ttgcgagacc tgcgcttcgg tgggaatggt
     4561 ctactggaat tcgcgtatgc accagctaac aggcgactcg aaatacatgg acgtgctgga
     4621 aaggtccatg tacaacggcg cgctggcggg cgtgtcgctg aagggagacc tgttctttta
     4681 cgtaaatccg ctggcttctt acggggacca tcaccgcaag gaatggtacg gcacggcttg
     4741 ttgccccagc cagatctccc gttttcttcc ttccatcgga aactatattt acggaacatc
     4801 ggaacgggcc gtctgggtaa atttgtacat cggaaacaaa gcagacgtga acaccggaaa
     4861 agagacgatg accctggtcc aggagaccag ctatccgtgg gacggcaacg tgacccttac
     4921 cgttgagtcg ctcaaaaaat ccgtcaggaa agaattccgg ctgcgaatcc ccggatggtg
     4981 caaaaactac accgttgccg taaacggaga gtcaataacc gatccgttga ttgaaaaggg
     5041 atacctggtg atcgaccgga aatggaaatc gggagacaag gtaacgcttg ccctggacat
     5101 gccggcagaa acggtacagg ccgatccgcg ggtaaaagaa aactccggga aaagggctgt
     5161 tcaacgaggg ccgatcgtat attgcgcgga agaaacggac aacccttcgt tcgatgagtt
     5221 gacactttcc ccgcctgcca gattcaagga aacattcaac cccacgctgc tgaacggcgt
     5281 cactaccatc gacgccgttt cgggagatga caccatccgt tttatcccct attacgcgtg
     5341 ggacaaccgg gaggccggac aaatgaaagt gtggatgaat tataacgaat aagaataatc
     5401 gcatgagaca tttaattgcc atccttattt cggtcttcct gatcccgggc actcattcac
     5461 aaggaagtaa ctccggacaa ccacctaact accccggcaa ccgtgccccg ctgaccggta
     5521 aaacctatat cgaactgccc gtcggagcta tccgccctca agggtggctc aaagaacaat
     5581 tgaaccggat gcggacagga atgacaggac atctggactc catttacccg caggtcgccg
     5641 gacagagaaa cggatggctg ggcggtgacg gagacgtgtg ggaacgaggg ccgtactgga
     5701 ttgacggact cttgccgctc gcctatatca tgaacgatgc ggaattaaaa aagaaagtgc
     5761 agccctggat tgagtggacg ttggcatcgc aaaagccaaa cggatacttt ggcccggata
     5821 ccgaccggga acccgaagaa ggcctgcaac gaaacaacgc tcacgactgg tggcctaaaa
     5881 tggtgatgct gaaagtaatg cagcaatact attccgcaac cggggataaa cggataattc
     5941 cctttatgac aaattatttc aaatatcagt tgaaggagtt gccgaacacg ccgttgggta
     6001 actggacatt ctggggagaa cagcgcggag gagataacct gatgatggcc tattggttgt
     6061 acaacattac gggagatcct tttttgctcg agttgggtga gctgattcac gaacagacat
     6121 tcaactggac ggatgtattc ctccgtcagg atcatctatc gcgacagcac agcttacatt
     6181 gtgtaaatct cggacaggga tttaaagaac cggctatcta tttccagcag aatcaaaatc
     6241 ctgaactgct cgaagcggta aaaaaagctg tccgggatat gcgcaacacc atcggattgc
     6301 ccatcggatt atgggcgggc gacgaattgc tccgttttgg aaatcccgtt caaggttctg
     6361 agctttgcac cgcagtggag atgatgtatt cgctggagag tatcctacag gttaccgggg
     6421 atgtgcaatg ggcggaccac ctcgaaaaaa ttgcctataa tgccttaccc actcaaacca
     6481 ccgacgattt tgacgctcga cagtattacc agcaggtcaa ccaggttaaa gtcagccggg
     6541 acatgcggaa cttcgtgact ccccaccacg ataccgacaa ccttttcggt gaactcaccg
     6601 gatacccctg ctgtacctcc aacatgcatc aggggtggcc caagtttacg cagaatctct
     6661 ggtatgcaac caacgacaac ggcattgccg cactggttta cgccccatcg gaggtctcgg
     6721 caagggtggg aaatggacaa ctcatcaccg ttaaagagtc cacacagtat ccttttgagg
     6781 aaaccatcgc ctttaccttc gattttccgg acagagaaga agaagaaata gaatttccgt
     6841 tccacctgcg tattccccga tggtgtaaaa accccgtaat aacgataaac ggggaagggg
     6901 ttacggcaga aacaaggcaa ggtgaaatcg caaggatcga tcgtgcctgg aaaagcaatg
     6961 acaaggtcgt tctacgcctg cccatggaag tgtcgataag ccgatggtac gatggcggag
     7021 cggccgttga acgcggtcca cttctgtatg cgctgaagat gaatgaaaac tggcaaaaga
     7081 aaacaactga gccccacgaa accaaacgtt acggaaaatg gtattaccag gttaccagcg
     7141 actctccctg gaactatgcc ttacctatcc gtaacttaag caaggaaaat gttgggaaag
     7201 cttttgttgt tgaaacaaaa aacagggctt ctgattatcc ctggaccctg caagatgcac
     7261 ccgttacgat aaggacaact ggattacgac tgcccggatg gaaagaatac aacgggtcgg
     7321 ccggcccggt cccttatttc actcaacagg gagaggatgt tggcggggaa gaaaacattg
     7381 agcttatccc ctacggatgc agcactctac gaattgctgt tttccccgtg aggaacaaat
     7441 aagaaataat tattttgagt atctaacaga ctacttacaa tccctatcgt attattttat
     7501 gaaagcattg gttttagaca aatacatgga gttgaattat cgtgatttcc ctgatcctca
     7561 tatggaagcc gacgaagtat tgataagggt tcaagcctgc ggaatctgcg gaagcgacat
     7621 ccacggtatg gacgggagca ccggaagaag aatccccccg ttggtcatgg ggcacgaagc
     7681 cgcaggcata attacggaag ttggcactgc ggtcacagga tggaaagcgg gtgacagggt
     7741 tactttcgac tccacgatat atccgctcga tgactggttc acacgtcagg ggcattacaa
     7801 cctcagcaac aatagaaaag tcctgggtgt ttccccgggg aattatacaa aacacggtgc
     7861 ttttgccgaa ctcgtttctg ttccggcaca tatactttac aagattcccg acaacgtctc
     7921 ctttgagcag gccgcgatgg tagagcccgt tgctgtagcc ctgcatgcag taaatgtatc
     7981 cggtattcaa ctgggacaaa atgccgtggt tatcggcacg ggaaccattg gcctgtttgt
     8041 tgtaaaactg ctgcagacgg caggtgtata tcccatcatt gctatcgaca tggatgaaaa
     8101 aaaattagaa ctttccaagg ccttcggcgc gactcatact ttcctctctt ccgataaaac
     8161 cgtttcagag aaaatacaac aactaaccaa aggccggaac gcagatgtgg cttttgaagc
     8221 agtaggagtt tcggaaactg taaacctgtg tataaacagt ttacgcaaag gaggcaaagc
     8281 aattctgata gggaacctga tgcccgaggt gactgtcccc ctgcaaaaaa ttgtaaccac
     8341 agaactttca cttctgggaa gttgcgctat caatggcgaa tacgaaacgg tgttagaact
     8401 gatggcttcc ggcaaaatcg acgttggcgg catgatatcg gccgtggcac ctctttctga
     8461 gggtgctgaa tggtttaacc ggctttacag aaaggaagcc ggaataaaca aagtgatcct
     8521 gattccctaa ttcaatgtcg aaatgagaaa ttctgtttac aatatcgcaa ttctgggatg
     8581 tgggaaagtg gcccacctgc atgcttcggc aatacagaat ctgcctaatg cccgcctagc
     8641 cggggtctgg agccgcaccc aacaaacggc aaacggcttt gcctgtcaat acaaagttcc
     8701 cgcctacacc gatgtggcgg agctcattaa aaaagaaaaa attgacctgg ctgtcgtctg
     8761 tactcctcac ccgtttcatt tggagccggc acaacaagct gccctgcaag gagccaatat
     8821 cctggtggaa aaaccactgg catcgactct ggaagatgct gacaaaatta tttcaacctg
     8881 taaaaaggcg ggagtcaagc ttggagtagt cagtcaacgc aggtggtatg aacccgtatt
     8941 gagggtgaaa aaagccattg aggccggcaa gataggtaaa ccggtattgg gtaccgttaa
     9001 catgctgggc tggagagaca aaagctatta cgatgccgat aaatggcggg gaaactggaa
     9061 aacggaagga gggggagtcc tggtaaatca agcgccacac caattggata tcctgctttg
     9121 gtttatggga gaggtggaag aagtttacgg catctggaga aacctgaatc atccttatat
     9181 cgaggtggag gataccgcat tggctgtaat aaaattcagg aacggaggaa taggaaatgt
     9241 tatcgtgagc aactcgcaaa aacccggaat ttacggaaaa gtacatgtgc acggtgaaaa
     9301 cggcgcatcc gcgggtgtcc agaccgatgg tggcgccatg tttattgcag ggatgaaagg
     9361 gattctggaa cccccggtaa acgacctctg gactattccc ggagaagaag agctggcgga
     9421 gaaatggaaa atggaagatg tccggcattt tagcagcatc gatccgatga cccattacat
     9481 ggagcgtcaa atagaagatt ttttatttgc cctggacgaa aaccgggatc ccctggttac
     9541 cggagaggac ggacgcagga cagtggaact gttcacggct atttatcgct ccacccgcga
     9601 taatttaccc ataaagcttc ccctttatcc cgaaaacaaa aacgacatgg atggaagatc
     9661 agggttgtta taatcaaaaa tatgcaatta tttatttcga ttgtaactca tctgcaaaat
     9721 aatacttacc tttattgctc tttttagtaa ggcacccgtt tgtagtatcc attctacccg
     9781 gaataacttt ataaaacata aattctaatg aacaggaaag atttcattaa atccgctgca
     9841 gtcatagccg gagcagcagc cgtaacgcca tttgcatctg catccgtttc gcaaagtaag
     9901 ccgaagaaaa aaatcgcctt aaaaaagagt ttggggcttg gaatgatcaa agaagaacta
     9961 tcgttaaccg ataaattcaa actggtcaaa gagttgggct ttgacggtgt agaactaaac
    10021 agccctgtcg acttcaatat gtcagaaata cttgacgcaa aatcgaaatc agggattgaa
    10081 cttcccacgg ttgtaaacaa ggaccactgg agctcacctc tttctgatcc cgaccctgcg
    10141 gtgaggaaaa agtgtatcga ctctgttata aaatctttgc aggaggtgaa agagatgggt
    10201 ggagacacgg ttttagtcgt gccgggtgta gtaaacgaga aggtatctta cgagcaggca
    10261 tacattacct cccaacagtc aattcgggaa ttgatccctt acgcagaaaa aacagggatg
    10321 caaattgcag tggagaacgt ctggaacaat ttccttataa gccctgttga agcaaaaaga
    10381 tttatcgatg aaataaatca ccccctggtg ggttggtatt ttgatatcgg gaacgttttg
    10441 cggtacggct ggcccgaaca ttggatcaag acacttaact cccgcatcat gaaacttcac
    10501 atcaaagaat tcagtcggga acttatgaac tcaaaagggt tgtgggaagg tttcaatgtt
    10561 gatttactga aaggcgacaa caattggccg gtggtaatga aagccgtcag agagataaat
    10621 catcagggag gctggttaac agcagaggtt aacggaggag acaggaatca cctgaagcag
    10681 atctccgctc aaatggatga aattatatcg tacttgtaaa agtcaatata agcaaaagtt
    10741 tgaccaaact atctaacaaa taattgatat aatatgagaa acaaagattc attatcgaga
    10801 agaagttttt taaaaagttc ggttgtggcc ggaacagtag ggatgatggg aggaattcca
    10861 atgttgagtt cctgctcgac cgataaatcc aaaaaaggtg agctgacgct gcccaagcta
    10921 ctggagcagg ctcccgacgg aaaggtaatt aaagccggat taattggatg tggaggtcgc
    10981 gggaccggtg ccgccatgga cttcttgaac gcaggtccaa acctacagat tgtcgcgctg
    11041 ggggatgttt tccaggacaa gctggacagt tgcagggaga ttttgaaaaa agaacggaac
    11101 gttgaaatcc ctgatgagaa ctgttttctc gggtttgaca gttacgaaaa ggtaatcgat
    11161 tccggtgtgg atatggtttt gctttgtacc ccaccccatt ttcgtccggc ccatgttgag
    11221 gccgctgtga atgctcaaaa gcatattttc atggaaaaac cgatagccgt tgatccgttt
    11281 ggggtcagaa aggtattggc agccgtcaaa agagcaaaaa ccttgaacct aaacattgtc
    11341 agcggaacca tccgtcgttc tcaaaaggat tacatggaaa ccagaagaag ggttctcaat
    11401 ggcgagatcg gcgatattgt gggagcaaac atcatccgga acggaggggc attatggttc
    11461 cgcaccagaa agccggaatg gacagacatg gaatatatgc tgcgaaactg ggtaaacttc
    11521 tgctggcttt cgggcgacca cataaccgaa caatttatcc acgaagtgga cgttatgaac
    11581 tggtatttgg gtaaaaatcc cgttaaggca accggttggg gcgggcgcca aagaagggta
    11641 accggcgacc agtatgattt cttcagcatc gagtacttgt acgacaacgg tatgcgtacg
    11701 cattgtgctg ccagacagat tgatggctgt accaacgaaa aagtagaaca aatcaactgc
    11761 accaacggct acgcagatgc ttccgggaaa ttgtatgacc tgaacggaaa cattatctgg
    11821 gaatatccgc atcctgaaga aaccgacact caatcggagt ggaaagtgac gaatccgttc
    11881 gtccaggaac acatcaacct ggttacggcc attcgaagcg gaaatacaat taacgacggt
    11941 gaagatcagg cttattcatc cttggtcacc atcatgggtc gtatggcggc ttataccgga
    12001 aaagacatca cgtgggatga agtactgaac gccgatcttt acctggggcc caaaacctac
    12061 gtaatggggc ctgttgaaaa cattcctgaa attatcccgg tggcaggtgt gcccggaaaa
    12121 gaataaaaaa aaacgtatga aacatttcaa tatattgttt ttagccctga ttttcacggt
    12181 gagcggtttt gctcagccat ccgggcagac acagaacacg gaacctccgc gtgaaaaagg
    12241 acaaaagagt gtactgcagt tagctgtgga tcccattcct accgtcaggg tggggtttat
    12301 cggattaggt aatcggggta ccggtgcagt agggagatac acatttatcg aaggtgtgga
    12361 gataaaagcc ctgtgcgatt tagatgcaga taaggtgaaa agatcgcaaa atacgttggc
    12421 aaaaagagga cttaagcctg ctgacgaata tacaggggaa aacgcatgga aagaattatg
    12481 ccggcggaag gacatcgacc tgatctatat ttgtaccgac tggctccacc atacacccat
    12541 ggctgtatat gccatgcagc aaggaaaaca cgttgccatc gaggttcctg cagcaacttc
    12601 tatcgaagag tgctggcaat tagtcgatac agcagaaaaa acaagaaaac actgcatgat
    12661 gttagagaat tgctgttatg acttctttga attaaacacg ttgaatatgg ctcagcaagg
    12721 acttttcggg aatattgtcc acggagaagg cgcctatatt cacgacttaa gagagagcaa
    12781 cttccgttcc ggtgcttact gggacatgtg gagattgaag ttcaacacgg cacatacagg
    12841 caacccctac cccacacacg gactcggtcc gatatgccag gtgatgaaca tccacagggg
    12901 agacaagatg aattatttgg tttccgtctc ttccaatcag tttggaatga cggaatacgc
    12961 caaggagaag ttcggtgaaa attcggctca ggcaaagcag gagtataagc tcggtgatat
    13021 gaatacaacc gttataaaaa ccgaaaaagg gcggacaata atgatacagc acgacgttac
    13081 aagtccccgg ccctacagca ggctacacac cattagcggc accaaaggtt tttttcagaa
    13141 atacccaacc gggttgatcg cactggaacc gaacgctcat agctttctca cggaaacaca
    13201 aattgactcg ctaatgactc aatacgaaca cccgttcata aaagaaattg gtgaatttgc
    13261 caaaaaggtg ggcggccacg gcggcatgga cttcatcatg gattaccggc tgatctactg
    13321 cttaaggaac ggactgcctc tcgatcagga tgtttatgat gctgcagaat ggtcctgtct
    13381 ggttgagtta agtgaacaat ctgtattaaa cggaggaatg cctgtaaaaa tccctgattt
    13441 cacccgtggc gactggaata agttaagcgg attgaaattt gcccattgac caatcgttat
    13501 agaatattcg gaatagccac gttttacaat gatagatcgc aggatgaatt tttaataagt
    13561 tctcctagtg tgtttatgta aaccggcaat tccgaatatt cagtttaagt ttcgcgtgtg
    13621 ttaattacga acatatttca attcctgccg gataaaactt aggcaccttg gggctattca
    13681 gttggcttcc tgaaatgggc agataaaata gcctgaccag acaaattggt tgagtaaaat
    13741 tatcagcata atttaaattt tcaaacatga aaaaacaggt catattaatc agcattatcc
    13801 tctctgtatt cttattaaaa accagccgta cttcttcaca ggaattagtg gcaacatttg
    13861 gcaaatctat acacatgaag gatgtccttt caggaaagct taaaaacgcc aagccggtaa
    13921 aatggtatca ggttaatacc gaagcagatt cctggagggt tagtggggaa actctgaaat
    13981 gcaccggatt gcccattgga gtaatccgaa gcgaaaaaga gtacgaaaac tttattatgc
    14041 acattgaatg gagccatcgt gcgccgggtg gaaattcagg cacatttgtc tggagtaaag
    14101 ctcaaccggg tgagaaccga ctaccggacg gtgttgaagt acaaatgttg gatctggaat
    14161 ggatcagact caatacccgc gatggagtag agccgcctat cgcttatgta cacggtgaat
    14221 tattcggcgt tggaggagtc gaaatcatcc ctgaaaatcc acggggcaaa aggagtaagt
    14281 cgatagaaaa cagggtaaaa gggacaaagg aatggaatat ttacgaggta gtgtgtgttg
    14341 acgggaacat taagttgtct gttaatggaa agtttgtaaa cgggatcacc aattcatcac
    14401 aaaaaaaggg atacatatgc ctggaggcag aaggttccga aattcatttc aggaatatcc
    14461 aaatcataga attggactga accaaaaaaa tcaatttctg gacagggctc aactgtgact
    14521 aatcaaccag tatacaagaa aatggagatc atcattagtt aaagcaaacg aaaacttgga
    14581 aaaaaatatc aaaatattat gaaatacaaa ttaaccctgt tttttttcat ttactttctt
    14641 ggctattacg ggtactcaca agatttttcc ccatcttcat ttgctaataa tcaaggggaa
    14701 gggaagtgga tggtttataa ggacaacttc agggcgttgt accggatact gtataatgaa
    14761 gctgaagacc tgctcggcaa acgttcagaa tacgtttcaa ccattcaaac taaagcagat
    14821 tggattgact atcaaaaaga gctgaagcaa aagtacaggg cttcactgat gaaattcaaa
    14881 aaaacaccat tgaatacacg aataaccggt cagttggaaa gagaaaaatt cacggtagaa
    14941 aagattattt tcgagtcaca tcccggattt tatgtgactg gatgcctgtt tatcccgaag
    15001 aaaagacagc atcctgctcc cgccatcatc tattgttccg gacattcgga caatggattt
    15061 cgtagcgaag gttaccaacg ctcaataatt aatttcgtag aaaaaggatt tattgttttc
    15121 gcctatgatc ctatcggtca aggtgaaagg ttgcaatacc tggatgaaac tacgggaaaa
    15181 tcccaaattg gagggcctac gaccgagcat tcctacgccg gaatccaaac
//
LOCUS       DAOSDR010000017        14972 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrimonas sp. isolate AMR_MDS_1826
            NODE_1590_length_14972_cov_3.977341, whole genome shotgun sequence.
ACCESSION   DAOSDR010000017 DAOSDR010000000
VERSION     DAOSDR010000017.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863355
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrimonas sp. (wastewater metagenome)
  ORGANISM  Petrimonas sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Dysgonomonadaceae; Petrimonas.
REFERENCE   1  (bases 1 to 14972)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14972)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:10:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,720
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 35
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14972
                     /organism="Petrimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1590_length_14972_cov_3.977341"
                     /isolate="AMR_MDS_1826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2023866"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..456
                     /locus_tag="PLI30_01345"
     CDS             <1..456
                     /locus_tag="PLI30_01345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019539415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HOI78229.1"
                     /translation="EKGYKKLMESIKRISKIEPAAHSSVNIDELRASCYSAMNDDFNS
                     PVLIAQLFEAVRVINSAVDKTETLTTADIDKLNALMQTFVFDVLGLTDESEANAGGDV
                     INGLMDLILDIRKSARENKDWVTSDKIRDQLKAAGVEVKDTKDGVEWRI"
     gene            492..1253
                     /locus_tag="PLI30_01350"
     CDS             492..1253
                     /locus_tag="PLI30_01350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019539409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaaA family protein"
                     /protein_id="HOI78230.1"
                     /translation="MQIVMSPAKRMNFNAQEENIKTTPPVFSRKTGEVLEVCRKLSET
                     DIAEKMKVNREIAQQVYGYFQSFNSRTIPLRAAALAYDGIAYKGLNAHDFNKEEVLFA
                     QKHLNILSGLYGALKPFDRIKPYRLEFARKIQPKGYKDLYEFWQNEVNQYLSGKLAKD
                     EKVIINVASKEYSSVLSKKLLPEKTRIVEISFLQQEGNDLKQIVVHSKKARGLMARFI
                     IKNRLNVAEDVKAFGYEDYFYYPQLSSENKWVFVR"
     gene            1266..2600
                     /locus_tag="PLI30_01355"
     CDS             1266..2600
                     /locus_tag="PLI30_01355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019539416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-dependent transporter"
                     /protein_id="HOI78231.1"
                     /translation="MNEKRSTFGSKLGMVAAAAGSAVGLGNIWRFPSETADGGGAIFI
                     IVYIACILFFGIPLMVAEFLIGRSSRANAAGAFHKLAPDTPWKWVGRLGVLTGFVILG
                     FYMVVCGWTVDYFIQSVTGSLKEVSDFSTNFNTLLANRPKQIGLMAFFVLLTAYFIFS
                     GVQKGIERSAKIMMPVLFLLLIVLVVRALSLEGARGGIDFLFKPDFQEVKSTVFLDAM
                     GQAFFSLSLGMGCMMTYASYFKDNTNLTATAVQVSVLDTLVALLAGMVIFPSAFALTT
                     NPDTIVSELVAGGPGLLFITVPGLFNQMGGSMIWSAMFFLLLAIAALTSTISLMEVVT
                     IYIQEEHKVSRKKGLLFVVIGVLALGALSALSPQFFNILDMASAKIMLPVGGLFISLF
                     AGWYLDKKIVQVELTNDGKIKFSISFLKVYSFILKFLAPVAILLIFLYGLIG"
     gene            2823..3014
                     /locus_tag="PLI30_01360"
     CDS             2823..3014
                     /locus_tag="PLI30_01360"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="HOI78232.1"
                     /translation="MTVQISRDGGVSWQPNVLVYDGPSAYSDMTVFRNGDVGIVYENG
                     LENPYEKITFLRMKRKRFK"
     gene            3086..4309
                     /locus_tag="PLI30_01365"
     CDS             3086..4309
                     /locus_tag="PLI30_01365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005935483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HOI78233.1"
                     /translation="MQYKIYPPEKLKTTIELPASKSISNRVLILNALSLNTNPVENLS
                     DCEDTQVIIDAFNSDSNVFDVKGAGTAMRFLTAFLAGMDGEWIVQGSKRMHERPIYPL
                     VDTLKAIGADIEYLKNEGCPPLKIRGKRLQGGEVYVSGSVSSQFISALMMVAPTMENG
                     LIINIRDEIISKPYISLTAKLMEEYGIHLKWEGNRIKIKPQSYKPVSFKVESDWSAAS
                     YWYEMVALCPDAEITLLGLKENSYQGDANLISLFKDLGVSTEFVSNGVVIRKAGKPIK
                     KFFHNFIYEPDLAQTFAATCCFLHVPFIFSGVQSLRIKETDRIEALKTELKKLGFVLR
                     ETDSEMLEWDGERCFVEENAVMDTYDDHRMAMSLSSAAILFKSLTMNDPHVVSKSYPN
                     FWDDLRKAGFRIEEV"
     gene            4384..4830
                     /locus_tag="PLI30_01370"
     CDS             4384..4830
                     /locus_tag="PLI30_01370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005678687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase"
                     /protein_id="HOI78234.1"
                     /translation="MTSLYILLGIIVFFLMALYISNTIQRKKGNTQERKTPLPSIDVR
                     TKQEKESGCCGMHEVCEKDSLIAAFREEPEYFDDEELDRFHFRESGSYSDKEVEEFRE
                     VFYTIVDEEKPRWVRSLQLRGIAVPDQIKDEIVLVVSDLREAKMHA"
     gene            4839..5672
                     /locus_tag="PLI30_01375"
     CDS             4839..5672
                     /locus_tag="PLI30_01375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008143578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HOI78235.1"
                     /translation="MDIIELLDYAFFRNALLGSFFASIACGIIGTYVVTRRLVFISGG
                     ITHASFGGLGIGFYFGLNPIFSAMIFSVFSAFGIQWLSRKQGVREDSAIAVFWSLGMA
                     VGIMLTFLTPGYAPNLSEYLFGNILTITRTDIVSLAILTSVLFLFFAVNYHAIVSVSF
                     DTQFAQTRRVNTRFIEYAMMLFVAVTIVLSIRLVGIVLLMSLITVPQMTANLFTVNYS
                     RIIYLSVGVSFVACVVGLLLSYYLNVPSGAFIIFVLIVMFFAGKIVKIWNPGSSKQSS
                     A"
     gene            6164..8080
                     /locus_tag="PLI30_01380"
     CDS             6164..8080
                     /locus_tag="PLI30_01380"
                     /inference="COORDINATES: protein motif:HMM:NF012581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HOI78236.1"
                     /translation="MIIASVVAISVFNNGLIEFYYRGSWVTGEIPVRIDYLSAWFVLI
                     ISFTFATGAWYGIRYMMAYKSQSGNLASHAAAYLLAYTALVDICLVQNGLVFLILWEV
                     MALSSFVLVIFEHYKIRTLKAGINFLIQSHVSILFLTIAIMMVKVNTGTFDFAAISSS
                     TYTQPAAIAVGLFIFFFCGFAIKAGFVPFHTWLPLAHPAAPAHVSGVMSGVIIKIGIY
                     GILRMVLLIKCDMVFIGYFILFISVITGVYGVMLAIVQHNLKRLLAYHSIENIGIIGI
                     GIGLGCLGIGYNNPLLVASGFGGGLLHVLNHSLFKSLLFYVSGNVYQATHNLEIDTLG
                     GLIRKMPHSGYLFLIASLAICGLPPFNGFVSEFFIYNGLFNGLISNNFLFILFILFAI
                     LGLVLIGGLALICFTKAFGVVFLGNPRSSKPLAPVNESLIKAAPLYLIALFILIIGVF
                     PVLFGPMLIKTVYLFLPESGTQATLELNNTLQSLTAIGWYSLGFIGLTALLYFIRKLV
                     TAKRSQASDVTWGCGYTGSAEKTQYTASSFVRTYRKLAEPVLMIKRKKNEAAGLYPDR
                     ISQATHPYDKIEYWLIDKPLLFIRSFLKRFTFLQNGHIQAYILYGFVFVGLTILLPVI
                     VEKIIELVNFLNQL"
     gene            8082..8987
                     /locus_tag="PLI30_01385"
     CDS             8082..8987
                     /locus_tag="PLI30_01385"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF012373.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit H"
                     /protein_id="HOI78237.1"
                     /translation="MVSVILIIFASIFFVGIVTRTKSIASGRKGPGIMQNMKDIIRLL
                     KKGSVYSKTTSFIFQIAPTVYLASVLMAILMLPHGVHPGVISFSGDFVMFAYLLALGK
                     FLIIVSALDTGSSFEGMGASREALFAMLAEPAFFILMGSFALFTGHTSFSSIFNALDF
                     GSYISYMLGGLATFVLVMIAMIENSRMPFDDPKTHLELTMIHEVMVLDNSGFDLGVIT
                     FTTNLKFAMYGAIISNFFIGALPLILSIPLFMLIQFCFAVTVGVIESFIARYRMSHNP
                     EFIFILTSLSLLIFFGVLLILGKFM"
     gene            8999..9628
                     /locus_tag="PLI30_01390"
     CDS             8999..9628
                     /locus_tag="PLI30_01390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI78238.1"
                     /translation="MTDILLIALAMTLLYMSIADRLQTYLYILVLQGLILFGTTYLTL
                     TDVSTLNLLLVLLETIVFKAIAVPWFMNYLIKRNKITRVTEPYLPNFVSLIIVTLIIV
                     STIILSGSIEDKNLDKTFFVVALSTLFTGLYLVVSRKKIISHVVGYLVIENGVFVLSL
                     AIGNEMPMLLNLGIMLDIFASVLILGVFFNKIGDVIRDPDVNLLTNLKD"
     gene            9634..11058
                     /locus_tag="PLI30_01395"
     CDS             9634..11058
                     /locus_tag="PLI30_01395"
                     /inference="COORDINATES: protein motif:HMM:NF012581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HOI78239.1"
                     /translation="MTGIFLSGAVLVSIGLILNRNAVVNYLLVVGYLALLITTTVYEY
                     INQGITQLVYFTSDSLGLLMLIAMTIVSIPAFYYGYRFIATRNDAPHARGLFFAAMVM
                     LITSCCMAYLSNHIAVTWIFVEITTLSASVLIYHNRSMRALEGVWKYVFLCAISITFI
                     YIGILFLSLSLKSAGVGEMSFKVLYENHHNLNVFWLKLAFLFIFTGFTAKMGLVPMYT
                     AGIDAKDKAPAPAGALLASVLMNVGFVGIYRVYAIVAHTPLKGWANSVIIIVGLLSVF
                     VAAVYMTRIKNIKRLFAYSGIEHMGLVVLGLAAGGVGVYAAILHIILHTFIKPTLFFQ
                     FNQIYTIYQSKSVYDLGNYFRYYKTGAFVVLLGFLSATAMPPSGLFVSEFMIFQSLFQ
                     SHYVVLLVVVLLLLTLIIWGFGVNIFKLLFTPTGQINEDKLPYISPWESLPQFVLLAL
                     SVYLAYNPPAEFVVLIQESVKLVQ"
     gene            11132..12700
                     /locus_tag="PLI30_01400"
     CDS             11132..12700
                     /locus_tag="PLI30_01400"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009134415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HOI78240.1"
                     /translation="MKYISISNNQAVSLDSIPELPYEEFLESNIGMLTDSPERHCVNY
                     FGFWTGVYSSGGPVEDQYDENKNRKEYSENVKLICCIADDKTHQIYVSSATVAPEVVY
                     ESFTARNHNFEKYEREMHETFGIQYNNHPWLKPVRYSHDRADKAETIENYPFFGADSH
                     ELHEVGVGPIHAGIIEPGHFRFICNGEQIMHLEIQLGYQHRGIEQLFLQKKKLAELTT
                     LAENIAGDTTVGHTIAFVNLWESLCGYKPGRSLHFSRTLALELERIAIHTGDMSAVCA
                     DIGYQLGNSVYGRLRTPIINFLQEWGGNRLAKGLIRAGRTNFPFSRKLGARLASILDE
                     FELDFNEMTSKFFKLPSALSRLEKTGVVTYGQVLEIGTVGMAARMSGLNRDIRSSHPF
                     NLFGEGFTHEPVIKHHGDVYARVRLRRDEIHQSIGYIRKMIADIPEFTEVAGVKLNPQ
                     RNMFILSLVEGWRGEICHCAITDNEGSLGLYKIKDPSFHNWMALAQAVRNNGISDFPI
                     CNKSFNLSYCGHDL"
     gene            12717..13466
                     /locus_tag="PLI30_01405"
     CDS             12717..13466
                     /locus_tag="PLI30_01405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019129252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone oxidoreductase"
                     /protein_id="HOI78241.1"
                     /translation="MFENIKIWWHQGKQYIPDVTNAKLPGIFRGRPVITRSGVDEAAL
                     VELCPTGAITRNPVCIDLGKCTFCGECAFRFPEKISFTGDYKLSANVRERLVVKEGDE
                     EGISVEPAKVRKVVRSLFGRSLKLRQVSAGGDNSCELELNACNNVQFDMSRFGIDFVA
                     SPRHADGVVITGPITANMAGPLEICYNAVPDPKIVILVGTDAISGGIFADSPALDRDF
                     LTKYPVDLYVPGNPAHPLTFINGVLDMLKKQ"
     gene            13614..14465
                     /locus_tag="PLI30_01410"
     CDS             13614..14465
                     /locus_tag="PLI30_01410"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009596099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase"
                     /protein_id="HOI78242.1"
                     /translation="MINKPIIIGIGELLWDMLPTGKKVGGAPVNFAYHASRLGAAGYA
                     VSAVGKDALGDEIIQEIDKVNINPVIERVNYPTGTVGVELNEGIPAYTINEGVAWDYI
                     PLTEAMKQLAKRADAVCFGTLAQRSEVSRNTIQALLSCVPVDSYRILDINIRQHYYSE
                     KVITDSLRSSNVFKMNDEELILLKKLFKKQPFTDEEVCHWFLKEFNLKFLILTAGANY
                     SIIYTPEGLSYIKTPVVNVVDTVGAGDSFTGAFISTVLHGKSVEEAHQSAVDRAAYVC
                     SQAGAWV"
     gene            14521..>14972
                     /locus_tag="PLI30_01415"
     CDS             14521..>14972
                     /locus_tag="PLI30_01415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI78243.1"
                     /translation="MFIVLLCTGCSTKRNTPATRAWHEFTTRYNIFFNAEEAYNEALK
                     SITESHIDNYQTWLPMYPNSADPNDTVVKQPGGPFDRVVEKMTTAIQEHSITAKPRRD
                     PAQPNTQEYRDWLRQNEFNPFIDRAWLLMGKAHVQNKDYTEAISVLSYT"
BASE COUNT         3967 a         2831 c         3505 g         4669 t
ORIGIN      
        1 gaaaaaggct acaaaaaact gatggaaagc atcaaaagaa tatcaaagat tgagcctgca
       61 gctcactcat cggtgaatat agatgagctt cgcgcaagtt gttattccgc catgaacgac
      121 gacttcaaca gtcctgtact tattgcgcag ctgtttgagg ctgtcagggt catcaattcg
      181 gcagtagata aaacggaaac cctcacgaca gccgacatcg acaaattaaa cgctttgatg
      241 caaacctttg tctttgatgt tcttggattg acggacgaga gcgaagcgaa tgccgggggc
      301 gatgtaatca atggcttgat ggatctgata cttgatattc gcaaatcggc ccgtgaaaac
      361 aaggattggg ttacctccga taagattcgc gatcaattga aagccgcagg tgtggaagta
      421 aaagatacga aggatggcgt ggaatggcgg atttgaggct tggcttttga ctgatcgaaa
      481 atctaaagaa tatgcaaatc gttatgtctc ccgccaagcg gatgaatttt aatgcgcagg
      541 aagaaaatat aaaaacgacc cctccggtct tctcccgaaa aaccggagaa gtgttggaag
      601 tgtgcagaaa actttcggaa actgatatcg ctgagaaaat gaaggtgaac cgcgaaatcg
      661 ctcagcaagt gtatggatat tttcaatcgt tcaactccag gacaattccg cttcgagcag
      721 ctgcgctggc ttatgacggt attgcttaca aagggttgaa cgcacatgat ttcaataagg
      781 aagaggtcct gtttgctcag aaacacctga acatcctctc cggactttac ggcgctctta
      841 aacctttcga ccggataaag ccttaccggt tggaatttgc tcggaaaatc caacccaagg
      901 ggtataagga tttatacgaa ttttggcaaa acgaagtaaa tcaatacctt tccggaaaat
      961 tagcaaaaga cgagaaggta atcatcaatg tagcttccaa agaatattca agcgttttat
     1021 ctaaaaagct tttacctgaa aaaacgcgga tcgttgagat aagcttcctt cagcaggaag
     1081 gcaatgacct taagcaaata gtggttcaca gcaaaaaggc ccgtgggttg atggcccggt
     1141 ttatcatcaa gaaccggctg aatgttgctg aggatgtaaa agctttcggt tacgaggact
     1201 atttctacta tccgcaactt tcctctgaaa acaaatgggt gtttgtcaga taaaaaaata
     1261 actgtatgaa cgaaaaaagg tccacgttcg gatcaaaatt gggtatggtg gcagcggcag
     1321 cgggttccgc ggttgggttg ggaaatattt ggcgcttccc gagtgaaacg gccgatgggg
     1381 gcggtgccat ttttatcatc gtctacatag cgtgcatcct gtttttcgga atcccgctga
     1441 tggtggcaga gtttttaatc ggacgttcat caagagccaa tgctgcaggc gcttttcaca
     1501 aactggcgcc ggacaccccg tggaaatggg tggggcggct tggggtgctc accggatttg
     1561 tcattctggg gttctatatg gtggtctgtg gctggactgt ggattatttt attcaatccg
     1621 ttacaggaag tttgaaagag gtgagcgatt tttcaaccaa ctttaacacg ttgcttgcca
     1681 accgtcccaa acagatcggt ttgatggcct tttttgtcct tcttacggcg tattttattt
     1741 tttcaggggt acaaaaagga attgaacggt cggcaaaaat catgatgccg gttttgtttc
     1801 tgttgctaat cgtccttgtt gtcagggcac tttcgctcga aggggcaagg gggggcatcg
     1861 attttctctt taagccggat tttcaggagg taaaatccac tgtttttctc gatgccatgg
     1921 gacaggcatt tttctccctt tccctgggaa tgggatgtat gatgacctat gcttcctatt
     1981 ttaaggacaa tacgaattta acagctactg cagtccaagt ttcggttctc gatactctag
     2041 tggcacttct tgccggaatg gtcattttcc cgtcggcttt tgcgttgacc acaaatcccg
     2101 ataccatcgt ttcggaactg gtggcaggcg gccccggatt gctctttatt accgttcccg
     2161 ggcttttcaa tcaaatggga ggatcgatga tctggtcggc catgtttttc ctccttttgg
     2221 cgattgccgc gcttacctcc accatttcgc tgatggaggt cgttacgatt tatatccagg
     2281 aagagcacaa ggtaagccgg aaaaaaggct tgctttttgt ggtcatcgga gtgttggcgc
     2341 tgggagctct ttctgccctt tccccacagt tctttaatat attggatatg gcttcggcaa
     2401 aaataatgct tcccgtgggt ggattgttta tctcgctttt tgcaggatgg tacctcgaca
     2461 agaaaattgt tcaggtggaa ctgaccaacg acggaaaaat aaaattcagc atctcattcc
     2521 tgaaagtata cagcttcatt cttaaatttc tggcgccagt tgccatcttg ttgatatttt
     2581 tatacggact aatcggatag caatgaagcg agcagccagt ggttctcaca cagcgagaat
     2641 cgattttggt gtttgccgaa gcccggaaga acggttgttc cgacacaggc aatatagact
     2701 tggtgatacg gcgttctact gacggcggcg cgtttttgtg atccattccg atactgacgg
     2761 attgagctgg tctgctcccg gcccggcttt tttccaatcc acgttcgacc gtgagggaga
     2821 agatgaccgt acaaattagt cgtgatggtg gggtatcgtg gcaacccaac gtattggttt
     2881 atgatggacc ctccgcatac tcggacatga cggttttccg taacggagat gttggaatcg
     2941 tttacgaaaa cgggttggag aatccttacg aaaagattac ttttctgaga atgaaaagaa
     3001 aacggtttaa ataatcctaa agacaaccca ttgctttggg actccgtaat ttattattat
     3061 ttttgcatca aaatagcggc acagcatgca atacaaaata tatccgcccg aaaagttaaa
     3121 aacaaccatc gaacttccgg catccaagag catcagcaac cgggttctca tcctgaatgc
     3181 gcttagcttg aataccaacc cggtggagaa tctttcggat tgcgaggata cacaggttat
     3241 tatcgatgct ttcaactccg attcaaatgt gtttgacgtg aaaggtgcag gaacggccat
     3301 gcggtttctg acggctttcc tggccggaat ggacggagag tggattgttc agggctcaaa
     3361 acgaatgcac gaacgtccca tttatccgtt ggtggataca ttgaaggcaa taggagcaga
     3421 tattgaatac ctgaaaaatg aaggttgtcc tccgttgaaa attcggggaa aaaggttaca
     3481 gggtggagag gtttatgtct ccggtagcgt cagctcgcag tttatttctg cgctaatgat
     3541 ggtggctccc actatggaga atggattgat catcaatatc cgggacgaaa ttatatcgaa
     3601 gccttatatc agtctgaccg ctaaactgat ggaagaatat ggcattcacc ttaaatggga
     3661 ggggaacaga ataaagatca agccgcagtc ttataaaccc gttagtttta aggtagagtc
     3721 ggattggtcg gccgcctctt actggtacga aatggtagcg ctttgccctg atgccgagat
     3781 taccctgctc ggattgaaag aaaacagcta tcagggcgat gctaacctga taagcctttt
     3841 taaggatctg ggcgtttcaa cggagtttgt ttcaaacggt gtggttatcc gaaaagcagg
     3901 aaagcccatc aaaaagtttt tccataattt tatctatgaa ccggacttag cgcaaacttt
     3961 tgccgcaacc tgttgttttc tgcatgtgcc gtttattttt tcgggtgttc aaagtttgcg
     4021 tatcaaagaa acggaccgta ttgaagcgct gaaaacggaa ttgaaaaaac tgggatttgt
     4081 cttgcgtgaa accgacagcg agatgctgga atgggacggt gaacgttgct ttgttgaaga
     4141 gaacgcggtt atggacacgt acgacgatca ccggatggcg atgtcgttgt catcggcagc
     4201 tattctgttt aagtcattaa caatgaatga tccgcatgtt gtcagcaagt cgtacccgaa
     4261 cttctgggac gatctcagaa aagcagggtt ccgtatcgaa gaggtttgac ataccgattg
     4321 actgataact ctatactttt ttgtctgtct gttttggtta taaaattctt taatctttaa
     4381 aaaatgacaa gtttatatat attactcgga attatcgtgt tctttctcat ggcgttgtat
     4441 atttccaata caatacaacg caaaaagggg aacacacagg aacgtaaaac gcctctcccg
     4501 tcgatagacg taaggacaaa acaggaaaaa gaaagcggat gttgcggtat gcatgaggtt
     4561 tgcgagaaag acagccttat cgctgctttc cgggaggaac ccgaatattt tgatgatgaa
     4621 gagctcgacc gttttcattt tcgggaatcc ggtagttatt ccgataagga ggtggaggag
     4681 tttcgcgaag tattctacac tattgtggat gaagagaagc cgcgatgggt acgcagcctg
     4741 cagctacgcg gaatcgctgt ccccgatcag ataaaagacg aaattgtgct tgttgtcagc
     4801 gatttacgcg aagcaaaaat gcatgcttga ccccacccat ggatatcatt gaattactgg
     4861 attatgcttt ttttcggaat gcattgctgg gaagtttttt tgcaagcatt gcgtgtggta
     4921 ttataggcac ctatgtggtt acgcgccggt tggtttttat cagcggggga atcacgcatg
     4981 cttcgtttgg cggactgggt ataggttttt atttcggact gaatcccatc ttttcggcga
     5041 tgatattctc ggtgttttct gcattcggta ttcaatggtt atcgcgaaaa caaggtgttc
     5101 gggaagattc tgccatcgct gttttctggt cgttgggaat ggcggtagga attatgctta
     5161 catttctcac gccgggatat gctcccaact tatctgaata tctttttgga aatatcctca
     5221 ctattacccg aacagatatt gtttctctcg ccatacttac ttctgtcctt tttctgtttt
     5281 tcgcagtcaa ttaccatgcc attgtatcgg tatcattcga tacgcaattt gcccagaccc
     5341 ggagggttaa tacccggttt atagaatacg cgatgatgct ttttgttgcc gtcaccattg
     5401 ttctaagcat tcggttggtc ggcatcgtgc tgttgatgtc gctcattact gttccccaga
     5461 tgacagccaa tctttttact gtgaattatt cccggatcat ttacctttcc gtaggagtca
     5521 gttttgttgc ctgcgttgta ggattgttat tgtcatatta cctgaatgtg ccgtcgggag
     5581 cgtttatcat tttcgttttg atcgtgatgt tttttgcggg aaaaatagtg aaaatatgga
     5641 atcccggttc aagtaagcaa tcttcagctt aaaaacaagc aattttattt tttctggtat
     5701 ttcttttgtc taaaccattt attttttata atatgacttt aaaaaactta ttgctagctc
     5761 aactattctt gcatttattt tgtataatca tcgatgtgta attttttctg tgtgccaacc
     5821 gaataatgta catgttaatt ttactgtgaa attcgaacaa gcgtgtactt cgataataat
     5881 gattggcctt ctgtacacaa tcgcttctta tggatttgct tttaatcgaa aggggttgct
     5941 tttcaaaaaa tgacaatata tggggtgtcg tataatgatt ttatgcagta tccgcgtatt
     6001 taatagaata gatgtttagt tgttagggat acttttcaat ggataaatgg aaaaattctt
     6061 agtagagttg ttaaatctaa ttgcgatagg agcaatttta tgttttcctc tttttaaaac
     6121 aaaaggaaag ggtatacttg ccgttagtgt gataacaata caggtgatta ttgcatctgt
     6181 ggtagcaatt tctgtattta acaatggatt gattgaattt tattatcgcg gttcatgggt
     6241 tacgggagag ataccggtaa ggattgatta tttatcggct tggtttgtgc tgataatcag
     6301 ttttactttc gcaacaggag cctggtatgg tatcaggtac atgatggctt ataagagtca
     6361 gtcaggtaat ttagcctctc atgctgctgc ttacttgctg gcatatactg cattggttga
     6421 tatctgcctg gttcagaacg gccttgtgtt tcttattttg tgggaggtta tggcgctgag
     6481 ttctttcgtt ctggtaattt ttgaacatta taagatcagg accttgaagg ctggaattaa
     6541 tttcctgata caatcacatg tttctatttt gtttctaacc attgccatta tgatggtaaa
     6601 ggttaataca ggtacttttg atttcgctgc tatctcttct tcaacctata cccaaccggc
     6661 tgctattgct gttggattat ttattttttt cttttgtgga tttgctatca aagcggggtt
     6721 tgtgccgttt cacacctggt tgcccctagc acatccggct gctcctgcac atgtttcagg
     6781 tgtaatgtcg ggagtaatta tcaagattgg tatatacggg atactgagga tggtattgtt
     6841 aataaaatgt gacatggttt ttataggcta ttttattttg ttcatctcag tcataacagg
     6901 tgtatatgga gtgatgttag ctatagtgca gcataacttg aaacggttgc tggcctatca
     6961 cagtattgag aatatcggga ttattggtat aggtataggg ttgggttgcc tgggtattgg
     7021 atacaataat ccgctattag tagccagtgg gttcggaggt gggttattgc atgtactcaa
     7081 tcattcgtta tttaagtcct tgttgtttta cgtttcaggt aatgtatacc aggcgactca
     7141 caatctggaa atagatacct tgggtgggct tatccgaaag atgccgcact ctggatattt
     7201 atttttaatt gcttcattgg caatatgtgg attaccccct tttaatggat ttgtatccga
     7261 gttttttatt tataatggcc tgttcaatgg gttgatatcc aataattttt tgtttatact
     7321 ttttatactt tttgcgattt tggggttggt gttgatcggt ggcttggcat tgatttgctt
     7381 tacaaaagca tttggagtag tgttccttgg caatccgcgt tcatcaaagc ctttagctcc
     7441 ggttaatgaa agtctcatta aagccgcacc gctatatctt attgccttgt ttatacttat
     7501 tattggcgta tttcctgttt tgtttggacc catgcttatt aaaacagtgt acttattcct
     7561 cccggagtca ggaactcagg cgacacttga attaaacaat acgttacaga gcctgaccgc
     7621 gataggatgg tactcgttag gttttattgg gttaaccgcg ttgctttatt ttataaggaa
     7681 actggttacc gcaaaacgca gccaggcttc tgatgttacc tgggggtgtg gttataccgg
     7741 aagtgctgaa aaaacacagt acaccgcttc ttcatttgtc agaacctatc gtaaactcgc
     7801 agagcctgtt ttgatgatta aaagaaagaa gaacgaagct gccgggttgt atccggacag
     7861 aataagccag gcaacacacc catatgataa aatagaatat tggttaattg ataaaccgtt
     7921 gttgtttata aggagtttcc tgaaacgttt tacatttctg caaaacggcc atattcaggc
     7981 atatatttta tacggctttg tttttgtagg actcacaatc ttgttacctg tgatcgtaga
     8041 aaagatcatt gaactggtaa atttcttaaa tcaattatag gatggtaagt gtcattttaa
     8101 tcatattcgc cagtatcttt tttgttggga tagtaacccg aacaaagagc atagcttcag
     8161 gacgcaaggg cccgggtatt atgcagaata tgaaagatat aattagacta ttgaaaaagg
     8221 gaagtgtcta tagcaaaaca accagcttta tatttcagat cgctccaact gtttacttgg
     8281 cctctgtatt gatggctata ttaatgctgc cgcatggtgt tcatccgggt gttatttctt
     8341 ttagcggtga ctttgtgatg ttcgcttatc tgttggcgtt aggtaaattt ctgatcatag
     8401 tttcggctct tgatacggga agtagctttg aaggaatggg ggcaagccgt gaagcactgt
     8461 tcgccatgct tgcagagcct gcgttcttta tattaatggg ttcttttgct ttatttacag
     8521 gccatacatc attcagtagc atctttaatg cacttgattt tggttcatat atctcttata
     8581 tgttaggggg attggccaca tttgtgctgg taatgattgc tatgattgaa aatagcagaa
     8641 tgccttttga tgaccctaag actcacctgg agctgacgat gatacatgag gtgatggtgc
     8701 ttgataatag cggatttgac ctgggcgtaa ttacttttac tactaattta aaatttgcta
     8761 tgtatggtgc cattatctca aatttcttta ttggagcctt gccattgatt ctttctattc
     8821 cattatttat gctgattcag ttttgtttcg ccgtgacagt gggtgttatt gaatctttta
     8881 ttgcacgata taggatgagc cataatcctg aatttatttt tatactgacc tcattatcct
     8941 tattgatatt tttcggggta ttgctgatcc ttggtaaatt tatgtaaacc gattgattat
     9001 gacagatata ttattaattg cattggccat gacactgctc tacatgagta ttgctgatcg
     9061 gttacagacc tatctgtata tactggtttt gcagggtttg atcctttttg gaactactta
     9121 cctgactctt accgatgtaa gtaccttaaa ccttctgttg gttttgcttg aaaccattgt
     9181 ttttaaagcc attgccgtac cctggtttat gaattacctg attaaacgta ataaaattac
     9241 ccgcgtaaca gaaccctatc ttcctaattt tgtgtcatta attattgtta cgctgattat
     9301 tgtatcaaca attattctct cgggttcaat agaggataag aatcttgata aaacattctt
     9361 tgttgtggca ttatcaaccc tattcacggg tctgtattta gttgtctcca ggaaaaagat
     9421 tatctcacat gttgtaggat accttgttat tgaaaacggg gtttttgttc tttctctggc
     9481 tattggaaat gagatgccta tgttgctaaa tcttggaatt atgcttgata tttttgcaag
     9541 tgtacttata ctgggagttt tcttcaataa aattggagac gtaatccgtg atccggatgt
     9601 aaacctgttg actaacttaa aagattaaaa acaatgacag gtattttttt atccggagct
     9661 gtattggtaa gtatcggttt aatccttaac aggaatgcag tagtcaatta tttattggtt
     9721 gttggttatc tggcattact tataacgaca acagtctacg agtatattaa tcagggaata
     9781 acccaattgg tttattttac ttctgattcg ttgggtctat taatgctgat agctatgact
     9841 attgtcagca tccctgcttt ttattatgga tatcgtttta tagcaacccg taatgatgct
     9901 cctcatgcaa gagggctatt ctttgctgcc atggtgatgc tgattacatc atgttgcatg
     9961 gcatatctat caaaccacat tgctgttacc tggatatttg tggaaattac tacgctgagc
    10021 gcttcggtgc tgatttatca taaccggagt atgcgtgcgc ttgagggagt atggaaatat
    10081 gtattccttt gcgccatcag tatcacattt atctatattg gtattttgtt tttgagtttg
    10141 tccctcaaat cggcaggtgt tggggagatg tcatttaaag tactgtatga gaatcatcac
    10201 aacctgaatg ttttctggct taagcttgca ttcctcttta tcttcacggg atttacagca
    10261 aagatggggc ttgtgcctat gtacacggca ggcattgatg caaaagacaa agctccggcg
    10321 cctgcagggg ctttgttggc aagtgtattg atgaatgttg gctttgttgg catatatcgc
    10381 gtttatgcta tagtagccca taccccgttg aaaggatggg caaattctgt tatcattatt
    10441 gtaggcttgc tttcggtttt tgtggcagct gtttacatga cccgaataaa aaatataaag
    10501 cgtttatttg cttattccgg tattgaacac atgggattgg ttgtgctggg gctggcagca
    10561 ggaggggtag gcgtgtacgc tgcaatttta catattatat tgcatacttt tattaaacct
    10621 acactgtttt ttcagtttaa ccagatatac accatttacc aaagcaagag tgtttatgat
    10681 ttgggaaatt atttcaggta ttataaaacc ggagcatttg tagtgctttt aggattcctt
    10741 tccgctactg caatgcctcc gtcagggctg tttgttagcg aatttatgat ttttcagtca
    10801 ctgtttcaat cgcattatgt ggttttactg gttgttgtat tgctactcct tacattgata
    10861 atctggggtt ttggtgtaaa tatatttaaa cttttattta ctcctactgg acaaattaac
    10921 gaggacaagt tgccttacat cagcccatgg gagtctttac cgcagtttgt tttgttagcg
    10981 ctgtcggttt atctggccta taatcctccg gctgaatttg ttgttttaat tcaggaatcg
    11041 gtaaaactag ttcagtaatc atgattaaat ttatgaatga tttaagtttc gggtacatcc
    11101 actcaatgag cagataactg aatataaaat tatgaaatat atttccatat ccaacaatca
    11161 ggctgtcagc cttgattcta ttccggaact tccttatgag gagttcctgg aaagtaatat
    11221 cggtatgttg actgattctc ctgaaagaca ttgcgtaaat tattttggtt tctggacggg
    11281 agtttattca tctggtgggc cggttgaaga tcagtatgat gaaaataaaa atagaaaaga
    11341 atacagcgaa aatgttaagc tgatatgttg tatcgctgac gacaagacac accagattta
    11401 cgtatcatca gctacggttg cccctgaagt tgtttatgag tcattcacag ccagaaatca
    11461 taacttcgag aagtatgaga gggaaatgca tgaaaccttc gggattcaat acaacaatca
    11521 tccgtggctc aaaccggtca ggtattcaca cgatagagcc gataaggctg aaacaattga
    11581 gaactacccc ttctttggcg cagacagcca tgagcttcat gaggtagggg ttgggcccat
    11641 acatgcaggc atcattgaac cggggcattt ccgctttata tgtaatggtg aacagattat
    11701 gcatcttgaa atccaacttg gataccagca cagggggata gaacaattgt tcctgcaaaa
    11761 aaagaaattg gctgaactga ccaccctggc agagaatatt gccggtgata ccacagtagg
    11821 ccatactatt gcgtttgtga atttatggga gagtttgtgt ggttataaac ccggccgctc
    11881 gcttcatttc tcgagaacac tcgcattgga attggagcgg attgctattc ataccggtga
    11941 tatgtccgct gtttgtgctg atatcggtta tcaacttggt aactccgtat atggcagatt
    12001 acgcacacca attattaatt tcctgcagga gtggggaggc aatcgtttag caaaaggatt
    12061 gatacgcgct gggaggacaa attttccgtt ttcccggaaa ttgggtgcga gattagcttc
    12121 aatactggat gaatttgagc tggactttaa tgagatgacg agtaaattct ttaaactgcc
    12181 atcagccttg tcacgtctcg agaaaacagg agttgtaact tatggacagg ttcttgagat
    12241 cgggactgtg ggcatggctg cacgaatgag tggtttgaac agagatatcc gttcctcgca
    12301 cccttttaac ctgtttggag agggatttac ccacgaaccg gtaatcaaac accacggtga
    12361 tgtttatgcc cgtgtcaggc tcagaagaga cgagatacac cagtcaatcg gctatatcag
    12421 aaagatgata gctgatatcc ccgagttcac agaggtagcc ggtgttaagt tgaacccgca
    12481 aaggaatatg ttcatccttt cgctggttga ggggtggcgg ggcgaaattt gtcactgtgc
    12541 cattacggat aatgagggga gccttgggct gtataagatt aaggaccctt cttttcacaa
    12601 ctggatggca ttggcccagg ctgtcaggaa taatgggata tcagattttc cgatttgtaa
    12661 taagagcttc aatttgtcgt attgcggaca tgatctgtaa gttaaaccgg taaattatgt
    12721 ttgaaaatat aaaaatatgg tggcatcagg gaaaacagta tattcccgac gttacgaatg
    12781 ctaaattacc cgggatattc aggggaaggc cagttataac aagatctggg gttgatgagg
    12841 ctgccctggt agagttgtgt cctaccggag cgattaccag aaatccggtt tgtattgact
    12901 tgggtaaatg tactttttgc ggcgaatgtg ctttcaggtt tcccgaaaag atatctttca
    12961 ccggtgacta taagctgtct gcaaacgtta gagaacgcct ggttgttaaa gaaggggatg
    13021 aagagggtat ttcggttgaa cctgccaaag tgagaaaggt ggtgcgttct ttgtttggcc
    13081 gctcattaaa actgaggcag gtgtcggcgg gaggagacaa tagttgtgag ttggagctga
    13141 atgcgtgcaa taatgtgcag tttgatatga gccggtttgg tattgacttt gttgcatcac
    13201 cccgtcatgc cgacggagtg gtgattaccg ggccaattac tgcaaacatg gccggtccac
    13261 tggaaatctg ctacaacgct gttcctgatc cgaaaattgt tattcttgtg ggtactgatg
    13321 ccattagtgg agggattttt gctgacagcc cggctcttga cagggatttt ctgacaaaat
    13381 atcctgtcga tctttacgtg cccggcaatc cagcccatcc gctgacgttt attaacggtg
    13441 tcctggatat gttgaagaag caatagaatg tttgtttgtc aatgaagaag agagaccatt
    13501 gatgaaagat ggaatactaa acgctgcttc gatacttttt tgtatttttg gaaagtaaaa
    13561 attgcatcag tgagttgaat cttcgtttgg gaataacttg acaaacagaa aatatgatta
    13621 ataaacccat tattatcggt ataggagagc tcctgtggga catgcttccc acaggaaaga
    13681 aagtaggggg cgcacccgta aactttgcgt accacgcttc ccgcctgggt gcggcaggtt
    13741 atgcggtcag cgcggtggga aaggatgcgc tgggtgatga aatcatacag gaaatcgata
    13801 aggttaatat caaccctgtc attgaaaggg ttaactatcc gacaggaact gtaggggttg
    13861 aactcaacga gggtattcct gcctatacga tcaatgaagg tgtcgcatgg gattatattc
    13921 ccctcaccga agcgatgaaa caattggcta aacgtgccga cgccgtgtgt ttcgggacat
    13981 tggcccagcg gtcggaagta tctcgcaaca ccatacaagc attgctttcc tgcgtccctg
    14041 tagattctta ccgcattctt gatattaaca tccgccagca ttattattcg gagaaggtca
    14101 ttacggattc gttgagaagt tctaacgtgt ttaaaatgaa cgatgaagaa ctgattttat
    14161 tgaaaaagtt gtttaaaaaa cagccattta cagatgaaga ggtttgtcat tggtttttaa
    14221 aagagtttaa tcttaagttt ttaatcctta ccgccggagc caactacagc atcatttaca
    14281 cgccagaagg cctatcatac ataaagacgc cggtagtaaa cgtggtagat accgtagggg
    14341 ctggcgattc gtttaccggt gcgtttatat caaccgttct tcatgggaaa tcagttgaag
    14401 aagcccatca gtctgccgtt gacagagctg cctatgtgtg ttctcaggca ggggcatggg
    14461 tgtaaaatgt tattccctat tctcaatatg cacgcatatt taaaacatat tgcagcagga
    14521 atgtttatcg ttctcctgtg taccggatgt tccaccaaaa ggaatacgcc cgctacacgt
    14581 gcctggcatg aatttaccac acggtacaat atctttttta atgcggaaga agcatacaac
    14641 gaggccttaa aatcaatcac tgaaagtcac attgataatt atcagacatg gttacccatg
    14701 tatcccaata gtgctgatcc caatgatact gtggttaagc aaccgggggg ccctttcgac
    14761 agggtagtcg aaaaaatgac gacagctatc caggagcatt ccatcacggc aaaaccgcga
    14821 cgtgaccccg cccaacccaa cacgcaggaa taccgtgact ggcttcggca aaacgaattc
    14881 aacccgttca tcgaccgggc atggctcctg atgggaaagg cgcacgtgca gaacaaggat
    14941 tataccgaag caatttccgt actgtcttat ac
//
LOCUS       DAOSDR010000018        14770 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrimonas sp. isolate AMR_MDS_1826
            NODE_1619_length_14770_cov_4.079375, whole genome shotgun sequence.
ACCESSION   DAOSDR010000018 DAOSDR010000000
VERSION     DAOSDR010000018.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863355
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrimonas sp. (wastewater metagenome)
  ORGANISM  Petrimonas sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Dysgonomonadaceae; Petrimonas.
REFERENCE   1  (bases 1 to 14770)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14770)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:10:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,720
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 35
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14770
                     /organism="Petrimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1619_length_14770_cov_4.079375"
                     /isolate="AMR_MDS_1826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2023866"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            245..1891
                     /locus_tag="PLI30_01420"
     CDS             245..1891
                     /locus_tag="PLI30_01420"
                     /EC_number="2.7.1.90"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047334 - diphosphate-fructose-6-phosphate
                     1-phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphate--fructose-6-phosphate
                     1-phosphotransferase"
                     /protein_id="HOI78244.1"
                     /translation="MSKSALQIARASYVPKMPQVLNGNVQPREGAATASVSDQEEIKK
                     LFPNTYGMPIITFEESRGEMGLENPVNVGVILSGGQAPGGHNVIAGIFDGIKRLNPDS
                     RLYGFILGPAGLINHEYKELTADIIDEYRNTGGFDIIGSGRTKLEEKEQFDKGLEILK
                     KLNIRALVIIGGDDSNTNACVLAEYYKSINAGVQVIGCPKTIDGDLKNGQIETSFGFD
                     TACKVYSEVIGNIQRDCNSARKYWHFIKLMGRSASHIALECALQTQPNICIVSEEVEA
                     KQQSLDDVVNDIATVVLHRAEKGMNFGTVLIPEGLIEFIPAMKALIAELNDFLAHNQA
                     EFDLVRRSGKRDYIIGKLSPKNSEIYASLPETVARQLTLDRDPHGNVQVSLIETEKLL
                     AEMVKTRLDEWKKEGKYSGKFATITHFFGYEGRCAAPSNYDADYCYSLGYTASMLVAA
                     GKTGYMSSVRNTTAPASEWIAGGIPITMMMNMERRHGEMKPVIQKALVKLDGNPFRYF
                     ASQREKWAIETDYVYPGPIQYFGPTEVCDQPSRTLKLEQQ"
     gene            complement(2000..>5850)
                     /gene="rpoC"
                     /locus_tag="PLI30_01425"
     CDS             complement(2000..>5850)
                     /gene="rpoC"
                     /locus_tag="PLI30_01425"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008661873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HOI78245.1"
                     /translation="VLEDKVAEKDLLSEEEYLNLLDTLPKDNHLLEDTDPNKFIAKMG
                     AEAILDMLERINLDKQANQLRHRASTDTSQQRKNEALKQLQVVEAFRGSKSVNKPDWM
                     IMKVIPVIPPDLRPLVPLDGGRFATSDLNDLYRRVIIRNNRLKRLIDIKAPDVILRNE
                     KRMLQEAVDSLFDNSRKSSAIKTDSNRPLKSLSDSLKGKQGRFRQNLLGKRVDYSARS
                     VIVVGPELKMHECGIPKDMAAELYKPFIIRKLIERGIVKTVKSAKKIVDRKEPVVYDI
                     LEYVMKGHPVLLNRAPTLHRLGIQAFQPKLIEGKAIQLHPLSCTAFNADFDGDQMAVH
                     LPLGNEAILEAQILMLASHNILNPANGAPITVPSQDMVLGLYYITKIRPGAQGEGMTF
                     YGEEEAIIAYNEGKVDIHALVNVLVDDIDENGDPVRKMHQTTVGRVITNEYIPKEVGY
                     INELLSKKSLRDIIGKVIKTCGVARTAQYLDDIKDLGYKMAFKGGLSFNLEDVIIPKE
                     KEDLIQQGYNEVEQIMDNYNMGFITYNERYNQIIDTWTHINSKLSNILMKQLAEDDKG
                     FNSVYMMLDSGARGSREQIRQLSGMRGLMAKPQKSGAEGAQIIENPILSNFKEGLSVL
                     EYFISTHGARKGLADTALKTADAGYLTRRLVDVSQDVIINEVDCGTLRGLVATDIKNN
                     DEVIASLYERILGRVSVHDVPHPTTGEILVSAGEEITEEIAEKIDKSPIERVEIRSVL
                     TCESRHGVCAKCYGRNLATGQMVQKGEAVGVIAAQSIGEPGTQLTLRTFHVGGIASNI
                     AAENRIITKYDGILEFEELRFVETQDAEGKTYKVVVSRLVEMRIIDPNTRIVLLSHNI
                     PYGSKLYFNDNDKVKKGDLICEWDPFNAVIISEATGKIKFENFLEGITYKVESDEQTG
                     LKEKVIIESRDKTKAPSAHIVNQDDDVLRTYTLPLGAHIVKGEDDEIKVGDVLVKIPR
                     AIGKSGDITGGLPRVTELFEARNPSNPAVVSEIDGEVSFGKIKRGNQEISITSKTGEI
                     KKYLVPLSKQILVQENDFVRAGMPLSDGAITPADILAIMGPTAVQEYIVNEVQDVYRL
                     QGVKINDKHFEVIVRQMMRKVEVVEPGDTRFLEQQLVDKHEMMEENDRIWGKKVIIDA
                     GDSQIFEPGQIVTVRKLRDENSSLKRRDLKLVESRDAIPATANQVLQGITRAALQTTS
                     FMSAASFQETTKVLNDAAINGKVDTLEGLKENVICGHLIPAGTGLREYEKIIVGSKED
                     FDKMNANRRSPLYQEVEEEA"
     assembly_gap    5851..5950
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<5951..6441)
                     /locus_tag="PLI30_01430"
     CDS             complement(<5951..6441)
                     /locus_tag="PLI30_01430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002558080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HOI78246.1"
                     /translation="MAFRKENKIKSSFSKITISLASPEQILESSFGEVLKPETINYRT
                     YKPERDGLFCERIFGPVKDYECHCGKYKRIRYKGIVCDRCGVEVTEKKVRRERTGHIH
                     LVVPVAHIWYFRSLPNKIGYLLGIPTKKLDSIIYYERYVVIQPGVLEDKVAEKDLLSE
                     EEYL"
     gene            complement(6467..>7012)
                     /gene="rpoB"
                     /locus_tag="PLI30_01435"
     CDS             complement(6467..>7012)
                     /gene="rpoB"
                     /locus_tag="PLI30_01435"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007212060.1"
                     /note="DNA-dependent RNA polymerase catalyzes the
                     transcription of DNA into RNA using the four
                     ribonucleoside triphosphates as substrates; beta subunit
                     is part of the catalytic core which binds with a sigma
                     factor to produce the holoenzyme; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HOI78247.1"
                     /translation="RMNLGQIFETVLGWAGKELGVKFATPIFDGASLDDLNEWTDKAG
                     IPRYGKTYLYDGGTGERFDQPATVGIIYMLKLGHMVEDKMHARSIGPYSLITQQPLGG
                     KAQFGGQRFGEMEVWALEAFGASHILQEILTVKSDDVVGRSKAYESIVKGEAMPQAGI
                     PESLNVLLHELKGLGLSVELD"
     assembly_gap    7013..7112
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<7113..10114)
                     /gene="rpoB"
                     /locus_tag="PLI30_01440"
     CDS             complement(<7113..10114)
                     /gene="rpoB"
                     /locus_tag="PLI30_01440"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007212060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HOI78248.1"
                     /translation="MSSNNTNQRINFASTKNPLDFPDFLEIQLKSFQDFLQLDAPPES
                     RKNEGLYKVFTENFPIADTRNNFVLEFLDYFVDPPRYTIEECIDRGLTYSVPLKAKLY
                     LYCTDPDHEDFAPVIQDVYLGTIPYMTDKGTFIINGAERVIVSQLHRSPGVFFGQSVH
                     ANGTLLYSARIIPFKGSWIEFATDINNVMYAYIDRKKKLPVTTLLRSIGFESDKDILE
                     IFDLAEEVKVSKANLKKVIGRKLAARVLKSWMEDFVDEDTGEVTSIERNEVIIERETI
                     LDQGHIDDILEADISSILLHKETSNSSDYSIIYNTLQKDPSNTEIDAIRHIYRQLRSA
                     EPADDASAREVINNLFFSEKRYDLGDVGRYRMNKKLGLDIDESIRVLTKEDIIEIIKY
                     LIELVNSKAVVDDIDHLSNRRVRTVGEQLYSQFGVGLNRMARIIRERMNVRDNEVFTP
                     IDLINAKTISSVINTFFGTHALSQFMDQTNPLAEITNKRRLSALGPGGLSRERAGFEV
                     RDVHYTHYGRLCPIETPEGPNIGLISSLCVYAKINDLGFIETPYRKVEDGVVNTRNEE
                     VVYLSAEEEEEKVVAQGNAPLNESGEFILPRVKSRLEADYPLATPEEVDLMDVSPMQI
                     ASIAASLIPFLEHDDANRALMGSNMMRQAVPLIKNEAPIVATGIEGRLVKDSRTQLMA
                     EGNGEIIYVDATTIKIKYDRTEEEEFASFEDSIKTYDIPKFRKTNQNTTIDLRPICKL
                     GQKVSKGDILTEGFATENGELALGRNLKVAFMPWKGYNFEDAIVLNERLVSEDVFTSV
                     HVEEFSLEVRETKRGLEELTSDIPNVSEEATKDLDEHGIIRVGARVKPGDILIGKITP
                     KGESDPSPEEKLLRAIFGDKAGDVKDASLKASPSLKGVVINTNLFSKAAKKGKSKLSN
                     KALLPKLDEEYENKMEELRKLLIDKLLHITEGKTSAGVKDYTNMDIVPKGAKFTLKVL
                     SDIDYQSVQLSKWTTDAHKNQLIRTTVI"
     gene            complement(10212..10589)
                     /gene="rplL"
                     /locus_tag="PLI30_01445"
     CDS             complement(10212..10589)
                     /gene="rplL"
                     /locus_tag="PLI30_01445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002558082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HOI78249.1"
                     /translation="MADLKAFAEQLVNLTVKEVNELAEILKTEYGIEPAAAAVAVAAG
                     PAAEAAPAVEEKTSFDVVLKSAGQAKLAVVKAVKELTGLGLKESKDLVDGAPSEIKKG
                     VTKDEADALKKQLEEAGAEVELK"
     gene            complement(10637..11161)
                     /gene="rplJ"
                     /locus_tag="PLI30_01450"
     CDS             complement(10637..11161)
                     /gene="rplJ"
                     /locus_tag="PLI30_01450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004310893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HOI78250.1"
                     /translation="MKKEDKNNIIEKIAANLQEYENFYLADIATLNAAKTSILRRECS
                     KQDIKLLVVKNTLLRKALEKLEGNYEELYPILKGNTAVMFSNDANAPAKLIDKYNKNK
                     EKAPALKGAYVQESFFIGAITLKDLVSIKSKTELIGEVISILQSPAKNVISALQSGGT
                     ILHGVLKTLSEKQN"
     gene            complement(11183..11881)
                     /gene="rplA"
                     /locus_tag="PLI30_01455"
     CDS             complement(11183..11881)
                     /gene="rplA"
                     /locus_tag="PLI30_01455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004310894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HOI78251.1"
                     /translation="MGKLTKNQKLALSKIEAGKTYTLKEAAKLVKEITTTKFDASVDI
                     DVRLGVDPRKANQMVRGVVSLPHGTGKQVRVLVLCSPEVEAAAKEAGADHVGLDEYIE
                     KIKGGWTDVDVIITQPQIMGKIGALGRILGPRGLMPNPKSGTVTPDVAKAVQEVKQGK
                     IDFKVDKAGIIHTSIGKVSFEPEKIVENAKEFIQTLIKLKPTAAKGTYVKSIYLSSTM
                     SPGLKVDSKSVDEI"
     gene            complement(11902..12345)
                     /gene="rplK"
                     /locus_tag="PLI30_01460"
     CDS             complement(11902..12345)
                     /gene="rplK"
                     /locus_tag="PLI30_01460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007755660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HOI78252.1"
                     /translation="MAKEVAGQLKLQIKGGAANPSPPVGPALGSKGINIMEFCKQFNA
                     RTQDKAGKVLPVVITYYTDKSFDFIVKTPPVAIQLLEASKQKSGSAEPNRKKVAEITW
                     EQVKTIAEEKMTDLNCFTLESAMKMVAGTARSMGISVKGEFPANN"
     gene            complement(12419..12961)
                     /gene="nusG"
                     /locus_tag="PLI30_01465"
     CDS             complement(12419..12961)
                     /gene="nusG"
                     /locus_tag="PLI30_01465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005844824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HOI78253.1"
                     /translation="MAESEKKWYVLRSVSGKENKVKEYLESEIKKTDLGKYVSQVLIP
                     TEKTYTVRNGKKVMKERAYLPGYVLIEAELVGEVIHQLKNINYVAGFLPNTTNPQPLS
                     EIEVKRILGTMDELEEAGDELDVKYYVGENVKVTSGPFSSFSGVVEEVNDERKKLKVM
                     VKIFGRAQLLELGYMQVEKE"
     gene            complement(12980..13171)
                     /gene="secE"
                     /locus_tag="PLI30_01470"
     CDS             complement(12980..13171)
                     /gene="secE"
                     /locus_tag="PLI30_01470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019538911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HOI78254.1"
                     /translation="MKKIVAYIKDAYNELVHKVSWPSSKELAGSTIIVMIASVIIALI
                     VFGMDSAFEWIVKFLYGIL"
     gene            complement(13192..13264)
                     /locus_tag="PLI30_01475"
     tRNA            complement(13192..13264)
                     /locus_tag="PLI30_01475"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(13229..13231),aa:Trp,seq:cca)
     gene            complement(13323..14510)
                     /gene="tuf"
                     /locus_tag="PLI30_01480"
     CDS             complement(13323..14510)
                     /gene="tuf"
                     /locus_tag="PLI30_01480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008762026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HOI78255.1"
                     /translation="MAKEKFDRSKPHVNIGTIGHVDHGKTTLTAAITTVLAKKGLSEL
                     RSFDSIDNAPEEKERGITINTSHVEYQTANRHYAHVDCPGHADYVKNMVTGAAQMDGA
                     IIVVAATDGPMPQTREHILLARQVNVPRLVVFMNKVDLVDDPEMLELVEMEMRELLSF
                     YDFDGDNTPVIQGSALGGLNGDPKWEDKIMELMDAVDNWIQLPPRDIDKPFLMPVEDV
                     FSITGRGTVATGRIETGVINTSDEVQIIGLGAEGRKSVVTGVEMFRKILDRGEAGDNV
                     GLLLRGIDKDEIKRGMVIAKPGSVKPHDHFKATVYILKKEEGGRHTPFHNKYRPQFYI
                     RTLDVTGEIELPEGIEMVMPGDNLEIDVKLIYPVACSEGLRFAIREGGRTVGSGQITA
                     LLD"
     gene            complement(14623..14694)
                     /locus_tag="PLI30_01485"
     tRNA            complement(14623..14694)
                     /locus_tag="PLI30_01485"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(14660..14662),aa:Thr,seq:ggt)
BASE COUNT         3506 a         3427 c         3341 g         4296 t
ORIGIN      
        1 gcaggggagt ggttacttcc attgaacaat cgggaggaga taaacgggct tttgtggatt
       61 ttgactctgc cggacagaag caactgctgc tgaagtttgc caaatttaaa attgtacaat
      121 gaaaatgaag aggataaaaa tagcattacc aatcgtttga gcatctcaga aaaaattgct
      181 aaatttgaac tctttttcag gcaaaccatc aataagaaat aaatctatta aatattagaa
      241 caaaatgtca aaaagtgctt tacaaattgc aagggccagt tatgtcccca agatgccaca
      301 ggtcctgaac ggtaatgtgc agcccagaga gggtgcagct actgcatcgg tgagcgatca
      361 ggaggagatt aaaaaactgt ttcccaacac gtacggaatg cccatcatca cttttgaaga
      421 atcccgggga gaaatgggat tggagaatcc cgtaaatgtg ggtgttatcc tatccggagg
      481 acaagctccc ggcgggcaca acgtgattgc cggaattttc gacgggatca aacggctgaa
      541 ccctgatagc aggctttatg ggtttatcct gggtcctgca ggactgataa accacgaata
      601 taaagaactt actgcggata ttatcgatga atatcgaaac acgggtggat ttgatattat
      661 tggttcggga cgtacaaagc tcgaagagaa agaacagttt gacaagggat tggaaatcct
      721 gaagaaactc aatatccggg cgctggttat tattggtgga gacgattcta acaccaatgc
      781 atgtgtgctg gccgaatatt acaaatccat taatgccggt gtgcaggtaa ttggctgtcc
      841 gaaaaccatt gacggtgacc ttaaaaacgg acagatagaa acatcgtttg gctttgatac
      901 cgcttgtaag gtctactccg aagtaattgg aaacatccaa cgcgactgta attcggcacg
      961 caagtactgg cacttcatta aactgatggg acgttctgcc tcacacatcg ccctggaatg
     1021 tgcgttacag acacaaccca atatttgtat cgtgtctgag gaagtggaag cgaagcagca
     1081 atcgctcgac gatgttgtta acgatattgc caccgtagtg ttacatcgcg cagaaaaggg
     1141 aatgaatttt ggaacggtgt tgattccgga aggtctgatt gagtttattc cagccatgaa
     1201 ggcgctgata gctgagttga atgacttcct ggcacacaac caggctgagt tcgatttggt
     1261 acgccgttcg ggaaaacgag attacatcat aggcaaactt tcacctaaaa attccgaaat
     1321 ttatgccagt cttcctgaaa cggttgcgcg ccagttaacg ctagaccgtg acccgcacgg
     1381 aaacgtacag gtttctctga tagaaacgga aaaactgttg gcggaaatgg tgaaaacccg
     1441 gctcgacgag tggaaaaaag aaggaaaata ttcgggaaaa tttgctacca taacccactt
     1501 tttcggatac gaaggccgtt gtgccgcacc gtcgaattac gatgcggatt actgctactc
     1561 gttgggatat actgcttcga tgttagttgc agcaggtaaa acaggataca tgtcatcggt
     1621 acgcaacaca actgctcccg cgtctgaatg gattgccggc gggataccca ttacgatgat
     1681 gatgaacatg gagcgtcgtc acggggagat gaaaccggtt atacaaaagg cactggttaa
     1741 gctcgatggc aatccgttcc ggtactttgc cagccaacgg gaaaaatggg cgattgagac
     1801 cgattatgtt tatccggggc ccattcagta tttcgggccg accgaagtat gcgatcagcc
     1861 gagcagaaca ttgaagctgg aacagcaata actcccttaa ccctacacac ctggataggg
     1921 gtgtaataaa caaaacccgc aggtggttcg caccatcctg cgggtttatt tatttaacag
     1981 cgtcgggata cctgtcccgt tatgcctctt cttctacttc ctgatacaag ggcgaacgcc
     2041 tgttagcatt catcttatcg aaatcttctt ttgagcctac aattattttt tcgtactccc
     2101 tcaagccggt tccggcagga atcaagtgac cgcaaataac gttctctttc agcccttcca
     2161 atgtatctac ttttccgttg atggctgcat cgttcagcac cttggtggtt tcctggaacg
     2221 aagcggctga catgaagctg gtggtttgca atgcggcacg tgtgataccc tgcaacacct
     2281 gattggcggt tgccggaatt gcatcgcggc tttcaaccag tttcaggtcg cggcgtttca
     2341 acgaactgtt ctcatcgcgc agcttgcgaa cggtaacaat ttgtcccggt tcgaagattt
     2401 gggaatctcc cgcatcgatg atcacttttt taccccagat gcggtcgttc tcttccatca
     2461 tctcgtgctt gtctaccaat tgctgctcca ggaaacgggt atcacccggc tcaaccactt
     2521 ctaccttacg catcatctga cggacaataa cttcgaagtg cttgtcgttg atctttacac
     2581 cctgcaaacg gtatacatcc tgtacctcgt tcacgatata ctcctgaact gcagtaggac
     2641 ccatgatcgc caaaatgtct gccggagtga tagcaccgtc ggataacggc atgccggcac
     2701 gtacaaagtc gttctcctgt acaagtatct gttttgacag cggaacaagg tatttcttga
     2761 tttctcccgt tttagacgtg atggaaatct cctgattacc gcgtttgatc ttaccgaacg
     2821 atacttcacc gtcaatttcg gaaacgacag ctggattgga ggggttacgc gcttcgaaca
     2881 attcggtaac gcgaggaaga cctcccgtaa tgtcacccga ttttccaatt gcgcggggaa
     2941 tttttaccag tacgtcgccc actttgattt cgtcgtcttc acctttcacg atgtgagcgc
     3001 cgagcggcaa tgtgtacgta cgaagtacgt cgtcgtcctg attcacgata tgggccgatg
     3061 gagcttttgt cttgtcgcgc gattcgataa tgactttctc tttcaatccg gtttgttcgt
     3121 ccgattctac tttgtaggta ataccttcca ggaaattttc aaacttgatc ttaccggttg
     3181 cttcggagat aattactgcg ttgaaggggt cccattcgca tatcaggtcg cctttcttca
     3241 ctttgtcgtt atcgttgaag tacaacttgg agccgtaggg aatattgtga gacaacagga
     3301 cgatccgggt gttggggtca atgatccgca tttccaccaa acggctcact accaccttgt
     3361 aggttttgcc ttcagcgtcc tgggtttcca caaaacgaag ctcttcgaat tctaagatac
     3421 cgtcatactt ggtgattatc ctgttttcgg cggcaatgtt ggaagcgata cctcccacgt
     3481 ggaacgtacg cagcgtaagc tgtgtacccg gttccccgat ggattgcgca gcaataacac
     3541 ccacggcttc acctttttgc accatttgtc cggtagccag gtttcttccg tagcattttg
     3601 cacaaacacc gtggcgggat tcgcaggtaa gtaccgaacg gatttcaacg cgttcgatgg
     3661 gtgatttgtc tattttttca gcgatctctt ccgtaatttc ttctccggca ctcaccagta
     3721 tttctccggt ggtggggtga ggaacgtcgt gcacggaaac tcgtcctagg atacgttcgt
     3781 aaagcgaagc aattacctcg tcgttattct tgatgtccgt ggcaactaat ccgcgaaggg
     3841 tgccgcagtc cacttcgttg atgatgacgt cttgggaaac gtccaccaaa cggcgggtca
     3901 gataaccggc gtcggccgtc ttcagtgcgg tatcggcaag accctttcgt gcaccgtgag
     3961 tggagatgaa gtactccaat accgaaagtc cctccttgaa gtttgaaaga atgggatttt
     4021 cgatgatttg agccccttcg gcaccgcttt tttgcggttt agccatcaaa ccacgcatgc
     4081 cggaaagctg tcggatttgc tcgcgtgaac cacgagcgcc cgaatcgagc atcatgtaca
     4141 cggagttgaa tcctttgtcg tcttcggcaa gctgcttcat gaggatattc gataacttgg
     4201 agttgatgtg cgtccatgta tcgatgatct gattgtagcg ctcgttgtag gtgatgaacc
     4261 ccatgttgta gttgtccatg atctgttcta cctcgttgta accctgttgg ataaggtctt
     4321 ccttctcttt cgggataatc acatcttcaa gattgaaaga caatccgccc ttgaacgcca
     4381 ttttgtaccc caggtccttg atgtcgtcca ggtattgggc ggtacgcgcc actccgcaag
     4441 tcttgatgac ctttccgata atgtcgcgga gcgatttttt cgagagcaat tcattgatgt
     4501 atcccacttc ttttgggata tactcgttgg taatcacacg cccaacggtg gtttggtgca
     4561 ttttgcgtac ggggtccccg ttttcgtcga tatcatcaac caaaacgttt accagtgcgt
     4621 gaatatccac tttcccttcg ttatacgcga ttatggcttc ttcttcaccg tagaaagtca
     4681 tgccttcgcc ctgtgctcca ggacggattt tggtgatgta atacaatccc aacaccatgt
     4741 cctgcgacgg aacggtaatg ggtgcaccgt tagcgggatt aaggatgttg tgcgaagcca
     4801 gcatcaggat ctgtgcttcc aggatagctt cgttgccaag cggcaagtga acggccatct
     4861 ggtcaccgtc gaagtcggcg ttgaatgccg tacacgatag cgggtgaagc tgaatggctt
     4921 tcccttcgat gagtttaggc tggaaggcct gaatacccaa acggtgaagg gtgggggccc
     4981 ggttcagaag gacagggtgt cctttcatca cgtactccag gatatcgtaa accacaggtt
     5041 ctttcctgtc aacgattttc tttgctgatt ttaccgtttt tacaataccg cgctcaatga
     5101 gcttgcggat gataaatggc ttgtagagtt ctgcggccat gtctttgggt atcccgcact
     5161 cgtgcatctt aagctcgggt ccgacaacaa taaccgaacg ggcggaatag tccacacgtt
     5221 taccgagcag gttctgacgg aagcgtccct gtttcccttt cagactgtct gaaagcgatt
     5281 tcagcggacg gttcgagtcg gttttaatgg cgcttgattt gcgggagttg tcgaacaaag
     5341 agtcaacggc ttcctgcaac atgcgttttt cgttgcgcag gatcacgtcg ggcgctttaa
     5401 tatcgatcaa ccttttcaga cggttgttgc ggataattac ccggcggtag aggtcgttga
     5461 ggtcggaagt ggcaaaacgt ccaccgtcca gcgggaccaa cgggcgtaag tcgggcggaa
     5521 tcacaggaat cactttcatg atcatccagt ccggcttatt aacgcttttg gatcccctga
     5581 aggcttccac tacctgaagt tgtttcaacg cttcgttctt acgttgttga gaggtgtcgg
     5641 tgcttgcgcg gtggcgcagt tggttggcct gtttatccaa gttgattcgt tccaacatgt
     5701 ccaggatagc ttctgcaccc atcttggcaa tgaatttgtt gggatcggta tcttccagga
     5761 ggtggttatc cttggggagc gtatccaaca agttgaggta ttcttcctcg ctcagcaagt
     5821 ctttttcagc tactttgtct tcaagtacgc nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5881 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5941 nnnnnnnnnn aggtattctt cctcgctcag caagtctttt tcagctactt tgtcttcaag
     6001 tacgcccggc tggatcacca catacctttc gtaatagatg atagagtcca gttttttggt
     6061 aggaataccc agcagataac ctattttgtt gggtaatgac ctgaaatacc agatgtgtgc
     6121 caccggaact accaggtgga tgtgtccggt acgctcacgg cgtactttct tttcagtaac
     6181 ttcaactccg caacggtcgc agacaatacc tttgtaacgg attctcttgt atttgccgca
     6241 atggcattcg taatctttta cggggccgaa aatgcgttcg caaaataaac cgtcgcgctc
     6301 cggtttgtag gtgcggtagt tgatggtctc aggttttaac acttcgccga atgaactttc
     6361 cagtatttgt tcgggagatg ccaaactgat ggttattttt gaaaagctac tctttatttt
     6421 attttctttt ctaaatgcca tattgttgtt ttataaagag ttattattaa tcaagttcta
     6481 cgctcaatcc caagccttta agctcgtgaa gcagtacgtt caacgattcg gggatgccgg
     6541 cttgcggcat tgcttctccc ttcacgatgg attcgtaagc ttttgaacgt ccgacaacgt
     6601 catcggattt aacggtaagg atttcctgca ggatgtgcga cgcgccgaaa gcctcgagcg
     6661 cccaaacctc catttctcca aaacgctgtc ccccgaactg ggctttcccg ccgaggggtt
     6721 gctgcgtgat gagcgagtat gggccgatgg accgggcgtg catcttgtct tccaccatat
     6781 gtccgagttt caacatataa atgatgccca cggtagccgg ttggtcaaaa cgttcgcctg
     6841 ttcctccgtc gtacaggtag gtcttcccgt agcggggaat accggctttg tccgtccatt
     6901 cgttcaggtc atctaacgat gcgccgtcga aaatgggtgt tgcaaatttt acgccgagtt
     6961 cttttccagc ccatccgagc accgtctcga aaatctgccc caggttcata cgnnnnnnnn
     7021 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7081 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnttgatgac cgttgtgcgg atcaattggt
     7141 ttttgtgagc gtcggttgtc cacttgctca attgaaccga ctggtaatcg atatccgaaa
     7201 gaaccttcag ggtgaattta gctcctttgg gaacaatatc catattggtg taatccttca
     7261 cgccggcaga cgtcttgcct tcggtaatgt gcaggagttt atcgataagc aactttctca
     7321 gttcttccat cttgttctca tactcttcgt cgagtttggg aagaagcgct ttgttcgaca
     7381 acttgctttt gcctttctta gcggcttttg agaacaggtt cgtattgata accacaccct
     7441 taagtgatgg cgaagctttt agagaagcat ctttcacgtc gcctgccttg tcgccaaaga
     7501 tggcgcgaag cagtttttct tcgggtgatg ggtcggattc ccctttcgga gtaatttttc
     7561 caatcagaat atcgcccggc ttaacgcgtg cacccacacg gataatcccg tgttcatcga
     7621 gatccttggt ggcctcttca cttacgttgg gtatgtcgga agtaagttcc tccaacccac
     7681 gtttggtttc acgcacttcg agagagaact cttccacgtg aaccgaagta aatacatctt
     7741 cgctgaccag cctttcgttc aaaacaatag cgtcttcaaa gttgtatcct ttccagggca
     7801 tgaacgccac tttcaggttc cggcccaaag caagctctcc gttctccgta gcaaaacctt
     7861 cggtcaggat atcgcctttg gaaacttttt gtcccaactt gcaaatcgga cgcaggtcga
     7921 tggttgtatt ctggttggtt ttacggaatt taggaatgtc gtaagtcttg atagaatcct
     7981 caaaactggc aaactcttcc tcctccgtcc tgtcgtattt tatcttgatg gtggtggcat
     8041 ccacgtaaat gatctctccg tttccttcgg ccatcaattg tgtacgggaa tctttaacca
     8101 gccgaccttc tataccggtg gcgacaatag gagcttcatt tttaataagc ggaaccgcct
     8161 gacgcatcat gttcgacccc ataagtgcgc ggttggcatc gtcgtgctca aggaacggta
     8221 taagtgatgc ggcaatagaa gcgatctgca tgggcgaaac gtccatcagg tccacttcct
     8281 cgggtgtggc aagcggatag tcagcttcga gcctcgattt cacacgcggt aaaataaact
     8341 cgccgctctc gtttaacgga gcgtttcctt gtgcaaccac tttttcttct tcttcttccg
     8401 ctgaaagata aaccacttct tcgttccggg tattaaccac accgtcttca actttgcgat
     8461 agggcgtttc gataaagccc aggtcgttga ttttggcgta aacgcagagc gaagaaataa
     8521 ggccgatatt gggcccctcg ggtgtttcaa tcggacacag acgtccatag tgcgtataat
     8581 gcacgtcacg cacttcaaaa ccggcacgtt cacgtgagag gccgccgggg cctaacgcag
     8641 aaagacggcg tttgttcgtg atctctgcca acggattggt ctgatccatg aactgcgaca
     8701 gcgcatgcgt gccgaagaac gtattgataa cggaagagat ggtttttgca ttgatcaggt
     8761 caatgggggt gaacacttca ttgtcacgca cgttcatcct ttcacggata atgcgcgcca
     8821 tgcggttcaa gccaacaccg aactgagagt atagttgttc cccaacggta cggacacgcc
     8881 tgttgctcag gtgatctata tcgtccacca ccgctttgga gttcacgagc tcgatgaggt
     8941 atttaataat ctcaataatg tcttctttcg ttaatacacg aatgctctca tcgatatcga
     9001 gacccagttt tttgttcatt ctgtagcgcc ccacgtctcc caaatcgtac cgtttctcgg
     9061 agaagaaaag gttgttgatc acttcgcgtg cactggcatc gtctgccggt tctgcactgc
     9121 gaagctgacg gtaaatatgc cggatggcgt caatttccgt attgctgggg tctttctgca
     9181 gggtgttgta tatgatggag taatcggacg agttggaagt ctccttgtgc aacaggatgg
     9241 aggagatatc cgcttccagt atgtcatcga tatgcccctg gtcgagaata gtttcccgct
     9301 caatgattac ttcgtttctt tctatggaag ttacttcgcc ggtgtcttcg tccacgaagt
     9361 cttccatcca cgatttcagc acgcgggcgg ctagttttct accaataacc tttttcaggt
     9421 tggctttgct tactttcacc tcttcggcaa ggtcgaagat ttcaaggata tctttatcgc
     9481 tttcaaaacc tatggagcga agcaaagtgg ttacaggtaa tttctttttt cggtcgatgt
     9541 acgcatacat cacgttgttg atgtccgtag caaattcaat ccatgaacct ttgaacggta
     9601 taatccgggc agagtagagg agcgtgccgt tggcatgtac gctttgaccg aagaatacac
     9661 cgggcgaacg gtgtaattga gatacgataa cccgttctgc tccgttgatg ataaaggtgc
     9721 ctttatcggt catgtacgga atggttccca ggtaaacgtc ctgaataacg ggtgcaaaat
     9781 cttcgtgatc gggatcggta caatataaat aaagtttggc cttcagcgga acactgtagg
     9841 taagccctct gtctatgcac tcttcaatag tgtagcgtgg cggatcgaca aaataatcta
     9901 aaaattcaag gacaaagtta ttacgggtgt ccgcaatggg aaaattttcc gtgaaaacct
     9961 tatacaatcc ttcatttttt ctgctttcgg ggggagcgtc aagttgcaaa aaatcctgaa
    10021 acgacttgag ttgaatttcc agaaaatcag gaaaatcgag tgggtttttt gtagacgcaa
    10081 aattgattcg ttggttggtg ttatttgaag acatgagagt aagtttttga acctaatatg
    10141 tattgacaca aaaggttaag aatcttccgg tagaagattc ctaaccaaat taacctggtt
    10201 aaaggcaaat cttatttaag ttcaacttct gctcctgcct cttcaagttg ttttttcaaa
    10261 gcgtcagctt cgtcttttgt tacacctttt ttgatttcgc tgggagcgcc gtcaactaag
    10321 tccttagatt ctttaagtcc aagtccggta agttctttaa ctgctttaac aacagctagc
    10381 ttagcctgtc ctgcgctttt aagaacaaca tcgaaagatg ttttttcttc cactgcagga
    10441 gcagcttcgg cagcagggcc agcagcaaca gcaacagctg cagcagcggg ttcaataccg
    10501 tactctgttt ttaatatttc agcaagttcg ttaacttctt ttactgttaa gttaactaat
    10561 tgttcagcaa atgcttttaa gtctgccatt tttgtattta ttaaattgat tactaattct
    10621 gtttgttgta tgaaaattaa ttttgctttt ccgacaacgt tttcaacact ccgtggagaa
    10681 ttgtaccgcc ggattggaga gccgaaataa cattctttgc aggtgattgc agaatgctga
    10741 taacttctcc gataagttct gttttgctct tgatacttac aaggtctttc agcgtaattg
    10801 ccccgatgaa gaagctttcc tgaacataag caccttttag tgcaggagct ttttccttgt
    10861 ttttgttgta tttatcaatg agcttagcag gagcattggc gtcgttggaa aacatgacgg
    10921 ctgtgtttcc tttcagtatg ggatacagtt cttcgtagtt tccttcaagt ttttccaatg
    10981 ctttacgcaa caaggtattc tttaccacta gcagcttaat gtcctgtttg gaacactctc
    11041 ttctgaggat gcttgttttt gccgcgttga gcgtggcaat atcagcaagg tagaagtttt
    11101 cgtattcctg caggttagct gcaatcttct ctataatatt gtttttatct tcctttttca
    11161 tagattcttc ttctttatac cattagattt cgtctaccga tttactgtcc acttttaatc
    11221 ccggactcat cgtacttgaa agataaatac ttttaacata ggtacctttt gctgccgtcg
    11281 gtttcaattt gatcaacgtt tgaataaatt cttttgcgtt ttcaacaatt ttttcgggct
    11341 caaaagatac ttttccaata gaggtgtgaa taatacctgc tttatctact ttaaagtcga
    11401 tttttccctg ctttacttcc tgaacagcct tagctacatc gggagtaacg gttccgctct
    11461 tgggattagg catcagtccg cgtggcccta aaatacggcc aagggcaccg atcttaccca
    11521 tgatctgcgg ctgtgtgatg atcacatcca catcagtcca acctccttta attttctcga
    11581 tatattcatc aagtcccaca tggtcggctc ctgcttcttt agcagcagct tcgacttcgg
    11641 gagaacacaa tacaagaact cttacttgtt taccggttcc gtgtggcaga gacacaacgc
    11701 ccctgaccat ctggttagct tttcgcggat ctacgccaag gcgcacatcg atatctactg
    11761 aagcgtcgaa tttcgtggtg gtaatttctt tcaccagttt cgctgcttct ttcagcgtgt
    11821 aggttttccc cgcttcaatc ttgctcaaag ccaacttttg attttttgta agtttaccca
    11881 tgtcttaatt gaagtttata attaattatt ggcgggaaat tcccctttta ctgagatacc
    11941 catgctccga gccgttccgg caaccatctt cattgcagat tcgagtgtga agcagtttaa
    12001 gtcggtcatt ttttcttcgg caatggtctt tacctgttcc caggtaattt ccgcaacttt
    12061 tttacggtta ggttctgccg atccgctttt ttgtttactg gcttctaaca actgaatagc
    12121 aacgggtggt gttttaacaa taaaatcaaa agacttgtcg gtataataag tgattaccac
    12181 aggcaacact ttaccggctt tgtcttgagt tctggcattg aattgcttgc aaaactccat
    12241 aatgttaatc cctttggaac ccaaggcagg tcctacgggg ggagatgggt ttgcagctcc
    12301 tcctttgatt tgtaatttta gttgtccagc aacttcttta gccattttct tgattttaat
    12361 aattactgtt ttcgaaacac ttgaagaatt tacagaatct gcgtaaccaa atttctgatt
    12421 attctttctc tacttgcata tagcccaact cgagaagttg agcccgtccg aagattttaa
    12481 ccatcacttt gagtttcttt ctctcatcgt tcacttcctc taccacgcct gagaagctgc
    12541 tgaaaggacc gctggtcact ttcacgtttt cgcctacgta gtattttaca tccaactcat
    12601 cgcctgcttc ctccagttca tccattgtac ccaggatgcg tttcacttca atttcgctca
    12661 acggttgtgg gttggtggtg ttgggaagaa atccggcaac atagttgatg ttcttaagct
    12721 ggtgaatgac ttctcccacc agttcagcct caataagtac atatccggga agataagcgc
    12781 gttctttcat cactttcttt ccgttacgta cagtataagt cttctctgtt gggatgagaa
    12841 cttgtgaaac gtactttcct aaatcggttt ttttaatttc tgactcaaga tattctttga
    12901 ccttattctc ttttccacta acggaacgca aaacgtacca tttcttttcg ctttcagcca
    12961 tttcttctca attcagaatt tacaaaatac cgtaaagaaa tttaactatc cactcaaagg
    13021 cagagtccat gccgaatacg attaaagcga tgatgacgga agctatcatc acaattattg
    13081 tactcccggc taattctttg ctggatggcc aagatacttt atgaacgagt tcgttataag
    13141 catctttaat atatgcaact atttttttca ttgattattc ggatcgtttt gcacgggtcg
    13201 agagactcga actcccgaca cctggttttg gagaccagtg ctctaccaac tgagctagac
    13261 ccgtaaaata caacccctcc aagctcctct gatgaggagg cttttgggag ttgtaaagct
    13321 ttttagtcta ataaagcagt aatttgtcct gaacctaccg tacgtccacc ttcgcggata
    13381 gcaaagcgaa gaccttctga gcaagctacc ggataaatca atttcacatc aatttccaag
    13441 ttgtcgcccg gcataaccat ttctatgcct tcgggtaact caatctctcc agttacgtcc
    13501 agggtacgga tgtagaattg aggacggtat ttgttgtgga acggtgtgtg gcgaccgcct
    13561 tcttctttct tcaggatata aacagtagcc ttgaagtgat cgtgaggttt cactgacccc
    13621 ggtttagcaa taaccatacc gcgtttaatt tcatctttat cgatgccgcg gagcaacaac
    13681 cctacgttat caccggcttc acctctgtca aggatcttgc ggaacatttc aactccggta
    13741 accactgatt ttctgccttc tgcacccaac cctatgatct gaacttcgtc actggtattg
    13801 ataacaccgg tttcgatacg gcctgtagcc acagttccac gaccggtgat ggagaacacg
    13861 tcttccaccg gcataaggaa tggtttgtca atatcgcggg gaggcaattg aatccagtta
    13921 tctactgcat ccatcagctc catgatttta tcttcccatt tcggatcgcc gttcaaacca
    13981 ccaagagcag aaccttgaat aacgggtgta ttgtctccgt caaaatcata gaacgaaagt
    14041 aattcgcgca tttccatttc caccagttcc aacatttcag ggtcgtcaac caggtctact
    14101 ttgttcatga aaacaaccag tctgggaacg tttacctgac gcgccaacaa gatgtgctcg
    14161 cgagtttggg gcataggacc gtctgttgca gctacaacga tgatggctcc gtccatttga
    14221 gcagcgccgg taaccatgtt tttaacatag tcggcgtgac cgggacagtc aacgtgcgcg
    14281 tagtggcggt ttgctgtttg gtattcaacg tgcgatgtgt tgatggtgat acctctttcc
    14341 ttttcttcgg gtgcgttgtc gatagaatcg aacgagcgca attctgaaag gccttttttg
    14401 gctaaaacgg tagtaatagc ggctgtcaag gtggttttgc cgtggtcaac gtgaccgatg
    14461 gtcccgatgt taacatgcgg tttcgacctg tcaaattttt cttttgccat aaaatatact
    14521 attaattagt ttaataaaat gttgaatttt ctatcttgta cgttttaaag tagctaaaat
    14581 aagatgccag accctcagca ccttcatttt tttcgttcca tgagccgatg gcgggaattg
    14641 aacccgcgac ctcttcctta ccaaggaagt gctctacccc tgagctacat aggcggtgag
    14701 agcggaagac gggactcaaa cccgcgaccc tcagcttgga aggctaatgc tctatcgact
    14761 gagctacttc
//
LOCUS       DAOSDR010000019        14604 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrimonas sp. isolate AMR_MDS_1826
            NODE_1635_length_14604_cov_3.763695, whole genome shotgun sequence.
ACCESSION   DAOSDR010000019 DAOSDR010000000
VERSION     DAOSDR010000019.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863355
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrimonas sp. (wastewater metagenome)
  ORGANISM  Petrimonas sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Dysgonomonadaceae; Petrimonas.
REFERENCE   1  (bases 1 to 14604)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14604)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:10:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,720
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 35
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14604
                     /organism="Petrimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1635_length_14604_cov_3.763695"
                     /isolate="AMR_MDS_1826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2023866"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(130..1182)
                     /locus_tag="PLI30_01490"
     CDS             complement(130..1182)
                     /locus_tag="PLI30_01490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005642133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2027 domain-containing protein"
                     /protein_id="HOI78256.1"
                     /translation="MKKMQLGDKVRFLNTVGGGQVKGFLNKQLVIVEDENGFDVPVLV
                     SECVVVEAAGNEKLGQPETEVPVKEPKPSTRLDETGPVTETAEGEKITTCLVYLPADA
                     RNMTQTSFECYFVNDSNYYLFLNYMSRENNSWKSRFNGIVEPNTQIFIEEFDKSQLND
                     LEKVCVQFVSFKHNKTFRFKSPVSVELRIDTVKFYKLHSFTENDYFDENALVYYITRN
                     DIPEREMLVSAGEIQRAMQEKTVSEERPRIQRIEKKEHQPAIEVDLHINELIDNTKGM
                     NNAEILEYQLAKFNEVMQENLKRKGQKIVFIHGKGDGVLKNTLLKEIKNKYKSCYYQD
                     ASFREYGFGATMVTIR"
     gene            1292..2533
                     /locus_tag="PLI30_01495"
     CDS             1292..2533
                     /locus_tag="PLI30_01495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006841724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosylmethionine:tRNA
                     ribosyltransferase-isomerase"
                     /protein_id="HOI78257.1"
                     /translation="MKKCDAEKIRIADYSYTLPPEKIARYPLEKRDSSKLLLFRNNTI
                     STRPFSGIGAEIPEGSLLVFNDTRVIHARLIFEKSTGAVIEIFCLSPHAPRDYSVNFQ
                     QRTSCSWHCMIGNAKRWKDDVLQMKVSVNNENVLLSARKIESNADDVIVEFSWNNPGF
                     TFSELLEIAGKLPIPPYLNRASEARDDETYQTVYSAVEGSVAAPTAGLHFTSEVMRSF
                     RKKGIQSARVTLHVGAGTFKPVKSETIGDHDMHMEFISVSRETVQALYENENNLIVVG
                     TTSMRTVESLYYLGKKILLQPDIQPEELVVFQWEPYGEENPVSPKLALKSIIDYLIRN
                     NADQLLAFTQVMIVPGYTFHYPQALITNFHQPQSTLLLLIASGIGKYWRDVYDYALQN
                     DYRFLSYGDSSLLWLTANQAI"
     gene            2759..3028
                     /locus_tag="PLI30_01500"
     CDS             2759..3028
                     /locus_tag="PLI30_01500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="oxaloacetate decarboxylase"
                     /protein_id="HOI78258.1"
                     /translation="MENINEALSLLLVGLTTVLIILSLVVLIGNGVIRFTNRFIPAET
                     MAVKKEFATGKAKPTNPAKIAAVIAAVEAITGGKGKIEKIDKIEK"
     gene            3046..4962
                     /locus_tag="PLI30_01505"
     CDS             3046..4962
                     /locus_tag="PLI30_01505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008773958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxaloacetate decarboxylase"
                     /protein_id="HOI78259.1"
                     /translation="MAKEIKFSLLYRDMWQSSGKYVPTVEQLLEVAPAIVDMGCFARV
                     ETNGGGFEQINLLFGENPNVAVRKWTQPFNDAGIQTHMLERGLNGIRMSPVPADIRRL
                     MFQVKKKQGTDIARSFDGLNDPRNLESSIRFAKEAGMISQAALSLTVSEVHTVEYYTE
                     LADTLIGMGADEICVKDMAGIARPATVGKIIKNIKEKHPDIPVTYHGHAGPGFQMASI
                     LEAAYAGVDYVDTAMEPLAWGTGHADVLAIQAMLKDAGFKVPDINMKAYMKVRSLTQK
                     YIDDFLGYYINPKNRLMNSLLIGPGLPGGMMGSLMADLESNLESLNKWLSKNGKEEIT
                     LDDLLIKLFNEVAYVWPKIGNPPLVTPFSQYVKNLALMNVMQIERGKERWSVIADNIW
                     DMILGRSGKLPGTLAPEIVQLIKEQGREFYNGNPQDLYPNQLDEFRAEMKKNNWDFGQ
                     DDEELFELAMHPEQYRAYKSGAAKKAFEEDLAKRKAAAQVEKTQPSPVEAPANGATNN
                     FQPRQLVINVDNEEYRVYISYPENDGIPFHPVNKDVPPQPKVVDSPSAVLPGGKVKEV
                     ISPLEGKFYLTKDSSETPLKVGSEVKIGEVIGYIESMKTYNAVSAEESGKVIEICFSN
                     GDSVEEDDILVKLQ"
     gene            4967..6175
                     /locus_tag="PLI30_01510"
     CDS             4967..6175
                     /locus_tag="PLI30_01510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009276413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium ion-translocating decarboxylase subunit
                     beta"
                     /protein_id="HOI78260.1"
                     /translation="MNEIFSNLVDMTAFGSISWGTLLMWIIAFILLYLGIKKQYEPLL
                     LVPIGFGVLLANLPLAGLGIVDNSLVQSPDGSYMNLLDIASEYGIMNFLYYALIKTGL
                     LPPIIFMGVGALTDFGPMLRNLKLSLFGGAAQLGIFTVLIIAVLVGFNMKEAASLAII
                     GGADGPTAIYTTIKLAPHLLGPIAIAAYSYMALVPVIIPLVANLLMTKKEFRIHMKKM
                     DKQFPPKHEIKHLKTVKIVFPIVLGVVVAVFVPSAAPLVGMLLFGNLVKEIGANTSRL
                     ADAASGPIMNTATIFLGLTVGATMTSEVFLSPRTLGIIVGGFLAFAISVAGGILAVKL
                     FNLFSKKKINPLVGATGLSAVPMASRVANEMALKYDKSNHILQYCMASNVSGVIGSAV
                     AAGVLISFLG"
     gene            complement(6178..7704)
                     /locus_tag="PLI30_01515"
     CDS             complement(6178..7704)
                     /locus_tag="PLI30_01515"
                     /inference="COORDINATES: protein motif:HMM:NF010184.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1846 domain-containing protein"
                     /protein_id="HOI78261.1"
                     /translation="MENFSRVGFDNEKYLQEQTSAILERVSRFNDKLYLEFGGKILYD
                     YHAARVLPGFTPNVKMRLLQKLKDQVEVIMCVYAGDIERSKIRADFGITYDVDVLKSI
                     DDLKWYGINVAAVVITRYEKQPAAVIFKNKLELKGIKVYLHYPTRGYPKNIDLIVSDE
                     GYGSNEYIETTKPIVVVTGPGPGSGKLATALSNLYHDYKKGKNAGYAKFETFPIWDLP
                     IDHLVNIAYEAATADLGDVNMIDPYHLKAYGLEAVNYNRDIEAFNLLKEILTKITGNN
                     SVYLSPTDMGVNRISAGIIDDERCKEAAYQEIIRRHLRGFVDYAMGQIDTETLERNAE
                     IMERVGAKDEDRLVVEPARRAAKDAETNGKGYGGIYCGAAIELNDGTLVTGKNSSLLH
                     ASSSMILNAIKHLAGLPDNMILLPENIIDSVTHLKKDILNGKNASLDVEETLIALAIS
                     AISNPAAQMALQKLTELNNCEMHSTHIPTPGDEAGLRKLKINVTCDPLFSSKRLFVSE
                     "
     gene            complement(7835..8620)
                     /gene="ygiD"
                     /locus_tag="PLI30_01520"
     CDS             complement(7835..8620)
                     /gene="ygiD"
                     /locus_tag="PLI30_01520"
                     /EC_number="1.13.11.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006798435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4,5-DOPA dioxygenase extradiol"
                     /protein_id="HOI78262.1"
                     /translation="MSTKMPVLFVGHGSPMIALEENNFTRGFLQVTAEIPKPDVIICI
                     SAHWETEGTFVTGMETPRTIHDFGGFPRELYEVQYPAPGNPELAGEIIDTLRQYSVGP
                     DYQWGLDHGTWTVLKHMYPDADIPVVQLSLDRSKTPQEHYSLARCLSDFRNRGILIMG
                     SGNMVHNLHLLDWGRINDDDYGFGWAISAAEKMYGYITANNHAPLIDYFTQGEDFRLS
                     IPTPEHYLPMLYALALQTDNEKVEFFNRGFVGGSLVMTALKIG"
     gene            complement(8709..9134)
                     /locus_tag="PLI30_01525"
     CDS             complement(8709..9134)
                     /locus_tag="PLI30_01525"
                     /inference="COORDINATES: protein motif:HMM:NF024812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOI78263.1"
                     /translation="MHIEQLKNIDDTVLKAFKTLIPQLIDRNSYPSREELQEILQAGN
                     TFLFIARENDEILGTLSLVVYKIPTGKKAWIEDVVVDENARGKGVAASLVYHALKVAR
                     EKGIEKVDLTSTPARVAANRLYQKLGFRKRETNVYRFTF"
     gene            complement(9296..11542)
                     /locus_tag="PLI30_01530"
     CDS             complement(9296..11542)
                     /locus_tag="PLI30_01530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010802950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOI78264.1"
                     /translation="MQHRHQHGRSDANIVGHILHQSTKEHIPFVTVSLKETSHATATD
                     ETGHYYLKDLPKGNFILVASGVGYKSVESPVTLEGGKTIEVNFVMEDDEVLLETVVVS
                     ANRTETKRKEAPSVVSVITPLLMESTNSVTLAQGLNFQPGLRIESNCQNCGFQQVRIN
                     GLDGPYSQILIDSRPIFSALAGVYGIEQIPANMIERVEVMRGGGSALFGSNAIAGTIN
                     IITKEPKTNNLSVSNVTNIIGASSPDNNLSFHASLVDNSLKTGLLVFASNRQREALDY
                     DDDGFTEIGKLRGNTMGFRGFYKFNNYNKLTIEYHFINEFRRGGNMLDKPPHTTDITE
                     QTEHNIHSANTNYNFLSRNYKHKINAYLSGQFIYRQSYYGTQRDPNAYGTTADNSMMG
                     GVQYSYSSERFLFMPSVVTTGVELNYNTMSDEMLGYNRKLDQEVNTKSTFLQNEWKNA
                     AWSILTGVRLDKHNLIKKAILSPRINIRYAPTPLVVLRGGYSTGFRAPQAFDEDLHIT
                     AVGGEVSIIQLSPGLKPEYSQTVSISADLYKAFGPVETNLLIEGFYTHLKDVFLLEEI
                     GKDPQGNLLKERRNGSGAVVKGVNIEGKIAPGRKFQLQMGGTIQESMYNKPESWSNNP
                     NVDPQRKMFRTPDIYGYFTATCTPTEAFNISLSGVYTGPMLVQHFAGYIPEDTEFRTP
                     GFFDSTIKLAYNFFATDKTTWQLNGGVQNILNSFQKDFDRGPLRDAGYMYGPSLPRTF
                     FAGIKLTM"
     gene            complement(11718..12083)
                     /locus_tag="PLI30_01535"
     CDS             complement(11718..12083)
                     /locus_tag="PLI30_01535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004317848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI78265.1"
                     /translation="MRQRFSILSGYFLLVLFVSYYGNISLFIHTHEVNGERIVHSHFN
                     FLAQDGNKTDHQHGTSSLNLIQALSHFYFTGLFLCAAYAALSKQISGYIRPKQEHFAL
                     KRVLIRANGWRAPPIQLHT"
     gene            complement(12177..12995)
                     /locus_tag="PLI30_01540"
     CDS             complement(12177..12995)
                     /locus_tag="PLI30_01540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019539233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HOI78266.1"
                     /translation="MKKSLLISLLISTSLSGIAQETNYRIIRNIPYYDDAKMESDAYI
                     RERCILDIYYPTDKKDFATVVWFHGGGLTGGEKELPDGFKHKNTCIIGVNYRLSPKVK
                     APAYIEDAAAAIAWAFKNIEKYGGDASKIFVSGHSAGGYLTCMAGLDKKYLAAHNIDA
                     NRIAGLIPLSAQTITHFNIREQRGIKNTQPVVDEFAPLFHVRHDAPPLLLITGDRELE
                     MLGRYEENAYLARMMKVAGHKKTRLLELDGYDHGMVYPALPLVMKEIENIIREE"
     gene            complement(13115..13591)
                     /locus_tag="PLI30_01545"
     CDS             complement(13115..13591)
                     /locus_tag="PLI30_01545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI78267.1"
                     /translation="MKTTILKLIISGVMLISLSSCETLTFGGKTVVKMKNDVAYQTIR
                     WDVNSSEADVEREFKAFIREILNRYRYKNYVQLFQSNYLLDEGERTYVIKIFQNEEAK
                     NNYLDHANDMIRRTTALANNRFSERWNQLQPGMIVERFSTPTVQNCNFPIDGCLSI"
     gene            complement(13655..>14604)
                     /locus_tag="PLI30_01550"
     CDS             complement(13655..>14604)
                     /locus_tag="PLI30_01550"
                     /inference="COORDINATES: protein motif:HMM:NF015573.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015501 - glutamate:sodium symporter
                     activity [Evidence IEA];
                     GO_process: GO:0015813 - L-glutamate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sodium/glutamate symporter"
                     /protein_id="HOI78268.1"
                     /translation="LKKGGTVLLIFLGLATIFSLLQNLVAIGLAKPLNLSPQLAMMTG
                     STALMGGLGTSAAIAPEVEATGISGAHAVAIASATFGLLMGSLMGAPIGNNLIVRKKL
                     LSTRKADEEEQKIDAMQAHPVPMDGERITKASFLILVSMGIGVIFTDLLNNFMGMFIG
                     MVKFPIYIGPMIVAAIIRNIMDTREDFTLNTEIQLIGDICLNIFISIAIMTMRLWELS
                     SLALPLMVLLLAQVMLMYVFPRFVAFPAMGRNYDSAVSTSGLIGFAMGSTANAMANLD
                     SICDKFGYSRVAYFIVPVVGAFFIDFINVLMITGFLAFL"
BASE COUNT         3795 a         3153 c         3410 g         4246 t
ORIGIN      
        1 ttcacactgc gataactgaa tggctccgcc aatctgatta agcacgtcac agcttgataa
       61 cgagaaaatc agcagtataa taagcactgt ttttttcatc tctccagggt tattttgtta
      121 tttgtttaat taccggatag ttaccatggt tgctccgaat ccgtattcgc ggaacgaagc
      181 gtcttgatag taacaacttt tgtatttatt ttttatctcc ttgagtagcg tattttttaa
      241 tacgccgtcg cccttcccat gaataaagac tattttctgg ccttttcgtt taaggttttc
      301 ctgcatcacc tcgttaaatt tcgcgagttg atattcgagt atttcggcat tgttcatgcc
      361 ttttgtgttg tctataagtt cattgatgtg taaatccact tcaattgcgg gttggtgctc
      421 tttcttttct attctttgta tgcggggacg ttcttctgaa acagtttttt cttgcattgc
      481 acgctggatt tctcctgcag aaaccagcat ttcccgttct ggaatatcgt tccgggtaat
      541 gtaatagaca agtgcatttt cgtcaaaata atcgttctcc gtaaaactgt gcagcttgta
      601 aaactttacc gtatcgatac gtaattcaac cgagaccgga ctcttgaagc ggaacgtttt
      661 gttgtgctta aacgatacaa actgaacaca aactttttcc agatcgttca gttgggattt
      721 gtcgaactct tcgataaaaa tctgtgtgtt gggttctacg ataccgttga aacgactttt
      781 ccaagagttg ttctcccggc tcatgtaatt gaggaaaagg tagtaattgc tgtcgttcac
      841 gaaatagcat tcaaacgaag tctgagtcat gttccgggca tcggcaggca ggtaaaccag
      901 gcaggtggtg atcttctcgc cttcggcggt ttcggtgacc ggccccgttt catcgagacg
      961 ggtagaaggc ttcggttcct taaccggaac ttcggtttcg ggttgtccca atttttcgtt
     1021 tcctgcggct tccacaacca cacattccga aacaagaaca ggtacgtcga atccgttttc
     1081 gtcttctaca ataaccagtt gtttgtttaa aaaccctttt acctgtcctc cccccacggt
     1141 gttcagaaaa cggaccttgt cgccaagttg catttttttc atttgaacgt gcagtttctt
     1201 atatgcattt tttgatgcaa acacaaagtt gcctaatttt gtcggattat ttacagaaat
     1261 tgtaataaaa aaaagactaa ttttcttaaa aatgaaaaaa tgcgacgcgg aaaagatcag
     1321 aattgccgat tatagctata cgcttcctcc cgaaaaaata gccagatatc cgttggaaaa
     1381 aagagattca tcgaaactgt tgctcttcag aaacaatacg atatctaccc ggcctttctc
     1441 cgggattggt gcagaaatac ccgaaggcag cttactggtt ttcaacgaca cccgtgtgat
     1501 tcatgcccgg ttgattttcg aaaaaagtac gggtgccgtt attgaaattt tttgcctttc
     1561 gccgcatgct ccaagggact attcggtaaa ttttcaacaa cgaacatcct gttcctggca
     1621 ttgtatgatt ggcaatgcca aacgctggaa agatgatgtg ttgcagatga aagtttctgt
     1681 aaataatgaa aatgtgttgc tttctgcacg aaagatagag agtaatgcgg acgatgtgat
     1741 tgttgagttt tcgtggaata atccgggttt tacgttttcc gaattactgg aaattgccgg
     1801 aaaactgccc attcctcctt atttaaatcg tgcttcggaa gcaagggatg atgaaacgta
     1861 tcaaacggtt tattctgcgg tggaagggtc ggtagctgct ccaacggcag ggttacactt
     1921 cacctccgaa gtaatgaggt cttttcggaa aaaaggaatc caatctgccc gggttacctt
     1981 gcatgtggga gcgggcacct tcaagccggt gaaatccgaa accatcggtg accacgatat
     2041 gcatatggag tttatctccg tttcaagaga aacggttcag gccttgtacg aaaatgaaaa
     2101 taacctgatt gtcgtaggga ccacgtccat gcgtaccgtg gagagtctgt actacctggg
     2161 gaaaaaaata cttcttcagc cggatattca accggaggaa ttagtggttt ttcaatggga
     2221 gccttacggt gaagaaaatc cggtttctcc taaactagcc cttaaatcca tcatcgatta
     2281 tcttattaga aataatgcag atcaattgct tgcctttact caggttatga ttgttcccgg
     2341 ttacacgttc cattatcccc aggcgctcat caccaatttc caccagcctc agagtacatt
     2401 attgttgttg attgcttcgg gtatcggcaa gtattggcgg gatgtttacg attacgcgtt
     2461 acaaaacgat taccgcttct tgagttatgg cgatagttca ttgctttggc ttacagccaa
     2521 tcaagcgata tagctgctat agtcctatgc ttattgaacg ataaggggta gaggtggatt
     2581 attggatatg ataaaaagaa gatagtataa cgctttttgt tttgatttga aaggtttggt
     2641 gctattttaa taaaaatatt ttagtatatt tgtaccttgg atttatactt cggaagcata
     2701 aatcttcttt cctaatatat gtattaatag gttatcaatt tgaaaataag ataattttat
     2761 ggaaaatata aacgaagcat tgagcctttt actggtaggt ttaacaactg tattgattat
     2821 tttgtctctg gtagttttga tcggtaatgg ggttattcgt tttaccaacc ggtttattcc
     2881 ggcagaaacc atggccgtga aaaaggagtt tgccaccggg aaagccaaac ctaccaatcc
     2941 cgcgaagatt gctgccgtta ttgctgctgt tgaagccata accggaggca aagggaaaat
     3001 agagaagatc gacaaaatag aaaaataaaa ataatcatac aaaacatggc aaaagaaata
     3061 aaattcagtt tactgtacag agacatgtgg caatcgtcgg gcaagtatgt cccgacggtt
     3121 gaacaactgc tggaggtggc tccggcaatc gtggatatgg gttgctttgc aagagttgaa
     3181 accaatggag gaggatttga acagatcaat cttcttttcg gcgagaaccc caatgtggcc
     3241 gtccggaaat ggacacaacc gtttaacgac gcaggtattc agacgcatat gctggagcgc
     3301 gggctgaacg gtatccgcat gagtcctgtc ccggctgata ttcgccggct gatgtttcag
     3361 gtaaagaaaa agcaggggac cgatatcgcc cggtcgtttg acggccttaa cgatccgcgt
     3421 aacctggaga gttccatcag gtttgccaag gaggctggta tgatttcaca agctgcgctt
     3481 agccttacgg tatcggaagt acacactgta gaatactata ccgaactggc tgacacgctg
     3541 attggaatgg gtgccgatga aatttgtgtg aaggatatgg cgggaattgc ccgtccggca
     3601 accgttggga aaataattaa aaacataaaa gagaagcatc cggatattcc cgttacttat
     3661 catggacacg caggacccgg attccagatg gcttccatac tcgaagccgc ttacgcaggc
     3721 gtcgattacg tggatacggc catggaaccg ctggcgtggg gaaccggaca tgcggatgtg
     3781 ctggctatcc aggccatgct taaggatgca ggctttaaag tgccggacat caatatgaaa
     3841 gcgtacatga aagttcgctc cctgacacaa aaatatatag atgattttct aggatattac
     3901 atcaacccga agaacaggtt gatgaactcg ttgcttatcg gacccggact tccgggcggt
     3961 atgatgggta gtctgatggc tgatttggag agcaacctgg agtcattgaa caagtggctg
     4021 tcaaaaaatg gcaaagaaga aattactctg gatgatttac tgatcaagct ttttaacgaa
     4081 gtggcttatg tatggcctaa gatcggaaat ccacctttag tcactccgtt cagccagtat
     4141 gtaaagaacc tggcactgat gaatgttatg cagatagaaa gaggcaagga gcgctggtcg
     4201 gtcattgctg ataatatatg ggacatgatc ctgggccggt cgggaaaact gccgggaacg
     4261 ttggcacctg aaattgttca gttgataaaa gaacagggca gggagtttta caatggaaat
     4321 ccgcaagacc tttatcctaa tcagctggat gagttccgtg ctgagatgaa aaaaaacaac
     4381 tgggattttg ggcaggacga tgaagagttg tttgagcttg ccatgcatcc cgaacagtac
     4441 agagcctaca agtcgggcgc tgcaaaaaaa gcttttgaag aggatctggc caagcgaaag
     4501 gctgcggcac aagttgaaaa aactcagcct tcaccggttg aagctccggc aaacggtgca
     4561 acaaacaatt tccaaccccg gcagctagtg atcaacgtag ataacgaaga atacagggtc
     4621 tatatatcct atcccgagaa cgacggtatt ccgtttcacc ccgtaaacaa agatgttccg
     4681 cctcagccga aagttgttga cagtccttcc gctgttcttc ccggtggaaa agtaaaagag
     4741 gtaatctctc ccctcgaagg gaagttctac ttaaccaaag attcttctga aacaccattg
     4801 aaagtgggga gtgaagtgaa aataggagaa gttatcggct acattgaatc catgaaaacc
     4861 tacaatgccg tatctgctga ggaaagcggt aaagtgattg aaatctgttt ctcaaacggt
     4921 gattcggtgg aagaggacga tatccttgta aaattgcaat gaagctatga atgagatttt
     4981 ttcgaactta gtagatatga cggcttttgg aagcatttcc tggggtacct tacttatgtg
     5041 gatcatcgct ttcatcctgt tgtatctggg tataaaaaaa caatatgagc cgttgctcct
     5101 tgttcctata gggtttggtg tgctgctggc aaacctcccc ttggctggtt tgggcatagt
     5161 ggataactcg ctcgttcaat cgcctgacgg aagttatatg aacctgcttg atattgccag
     5221 tgagtacggg attatgaatt tcctttatta tgcgcttatc aagactggac tgctgccacc
     5281 cattattttt atgggagtag gtgcattgac ggatttcgga cccatgcttc gtaacctgaa
     5341 actttcgttg ttcggtggtg ctgcgcaatt gggcattttt accgtgctta ttatagccgt
     5401 acttgttggt ttcaatatga aggaagccgc atcgttagcc attatcggtg gtgcagacgg
     5461 gcctacggcc atttacacta ccatcaagtt agcaccccat ttattgggtc ccatagctat
     5521 tgccgcttat tcttatatgg cgttggttcc ggtgattatt ccgttggtag ccaatttgct
     5581 gatgaccaag aaggaatttc gtatccacat gaagaaaatg gataagcagt ttccccccaa
     5641 gcacgaaatc aaacacctga agacggtgaa aattgttttt ccaatagttt tgggtgttgt
     5701 ggtagctgtt tttgtacctt ctgcggctcc gttggtgggg atgttgctgt tcggaaacct
     5761 tgtgaaggaa atcggggcca acaccagccg gctcgcagat gcagcttccg ggcccattat
     5821 gaatacggca accattttcc tcggattaac agtcggggca accatgacct cagaagtgtt
     5881 cctgtcgcca cggacgctgg gaattatcgt gggtggtttt ctggcgtttg ctatctccgt
     5941 tgcgggaggc atactggccg tgaaactgtt taacctgttt tcgaagaaaa agatcaaccc
     6001 tctcgtgggt gctacggggc tgagtgctgt cccaatggcc tcacgcgtgg ccaatgagat
     6061 ggcgttaaaa tacgataaat ccaaccacat cctgcagtat tgcatggcca gcaacgtgtc
     6121 gggagttatt gggtcggctg ttgctgcggg tgtattgata tcattcttgg ggtaagatca
     6181 ctcgctcaca aacaaccgtt tactggagaa tagcgggtcg caggttacgt tgatttttaa
     6241 tttacgtaac ccggcttcat ctcccggagt aggtatatga gtggagtgca tttcgcaatt
     6301 gttcagttcg gtcagtttct gcaatgccat ttgtgctgca gggttggata tcgcactaat
     6361 cgccaatgct ataagggtct cttccacatc aaggctggca ttcttcccgt tcaagatatc
     6421 tttcttcagg tgggtaaccg agtcgataat attttcgggc aggagaatca tgttgtcggg
     6481 taaccctgcc aggtgtttga tagcattcag gatcatactg gaggaggcgt gcagcaacga
     6541 tgaattcttt ccggtaacaa gggtgccgtc gttcagttca atggcagcgc cgcaatagat
     6601 tcccccgtat ccctttccgt tcgtttcggc atccttggcc gctcttcggg caggttcaac
     6661 cacaagacga tcttcatctt tcgccccgac tctttccatg atctcggcgt tacgttcgag
     6721 tgtttccgtg tcgatctggc ccattgcgta atcaacaaat ccccgtaaat gccggcggat
     6781 aatttcctga taagccgcct ctttgcatcg ttcatcatca ataatccccg cactgatccg
     6841 gtttaccccc atgtcggtgg gagagagata gacggaatta ttgccagtga ttttcgtcag
     6901 aatctctttc agaagattga aagcctctat atcgcggttg taattcaccg cttccaatcc
     6961 atatgccttc aggtggtaag ggtctatcat attaacatcg cctaagtctg cggttgctgc
     7021 ttcgtaggca atgttgacca gatggtctat aggtaaatcc caaataggga atgtttcgaa
     7081 tttagcgtag ccggcattct ttcccttttt ataatcgtga taaaggttgc tcagggctgt
     7141 ggcaagcttc ccgctgccgg gtccgggtcc ggtaaccacc acgatgggtt tggtggtttc
     7201 gatgtattca ttggagccgt atccttcgtc gctcacaatc aaatcgatgt ttttcggata
     7261 acctcgcgta ggatagtgca ggtaaacttt aatgcctttc aactcgagtt tgttcttgaa
     7321 gattactgcg gcgggttgtt tttcgtaccg ggtaataaca acggcagcta cattgatgcc
     7381 ataccatttc aggtcgtcga tgctcttcaa tacgtccaca tcataggtga tgccgaaatc
     7441 ggccctgatt ttgcttcgtt cgatatctcc ggcatacacg cacataataa cctctacctg
     7501 gtctttcagt ttttgcaaaa gacgcatttt aacgttcgga gtgaagccgg gcagaacccg
     7561 tgctgcatga taatcataca aaatttttcc tccgaattcg aggtataatt tatcgttgaa
     7621 ccggcttacg cgttcgagaa tagcagaggt ttgttcttgc aggtattttt cattgtcaaa
     7681 accaacacga ctaaaatttt ccatctttta attttccgaa taataaataa tagatacaac
     7741 ttactttcgg gactcaaaat tactcaaaat ttaggagaaa tccgccttaa atcgaggaaa
     7801 agtaagggag aagaaggtaa aacggataag ccttctatcc aattttcagc gcggtcatta
     7861 ccaatgatcc gccgacaaat ccccggttga agaattcaac tttttcgtta tctgtctgta
     7921 gtgcgagcgc atagagcatg ggcaggtaat gttcgggagt aggaatggag agccggaagt
     7981 cttcaccttg ggtaaaataa tcgataagcg gagcgtgatt atttgcggta atatatccgt
     8041 acattttttc tgcagcggaa attgcccatc caaacccgta atcatcatcg ttgatccttc
     8101 cccaatccag caggtgcagg ttgtgaacca tatttccgct tcccatgatc aagatgccgc
     8161 gattacgaaa atccgacaag catctggcca gggagtaatg ctcctgcggt gttttagaac
     8221 ggtcgaggct tagctgaaca accggtatgt ccgcatcggg atacatgtgt ttcagtacgg
     8281 tccacgttcc gtgatccagc ccccattgat aatcggggcc aaccgaatac tggcggagcg
     8341 tatcgattat ctctcctgcc agttccggat ttcccggggc aggatactga acttcgtaca
     8401 attcccgggg aaaccctccg aaatcatgaa tggtgcgagg tgtttccatc cccgtgacga
     8461 atgttccctc agtttcccaa tgagctgaaa tgcaaataat gacatcgggt ttgggtattt
     8521 ccgctgttac ttgcaggaac ccccgggtga agttgttttc ttccaaggca atcatcggac
     8581 tgccgtgtcc gacgaaaaga acgggcattt tagttgacat aaggcgattt tgtatataat
     8641 tttattaacc gttgaaacaa aaggctctgc gcaaggataa ccggacaaca gtagtatgtg
     8701 tctgtccatt aaaaagtaaa ccggtatacg ttggtctctc ttttcctgaa tcctaacttt
     8761 tggtaaagcc ggttagccgc tacgcgggca ggagtggagg tcaggtctac tttttcgatg
     8821 cctttttcgc gtgcgacttt caacgcatga tataccaatg acgcagctac tccttttccc
     8881 cgggcgttct catccaccac tacgtcttcg atccatgctt ttttccccgt gggaatttta
     8941 taaacgacaa gcgatagtgt acctaaaatt tcatcgtttt cccgggctat gaaaagaaaa
     9001 gtatttccgg cttgcagaat ttcctgcaac tcttcccggg aaggataact gtttctatcg
     9061 ataagctggg gtatcaatgt tttgaacgct ttcagaacag tgtcgtcaat attttttaat
     9121 tgttctatgt gcatgtcctc gttttttgac tgtgtggaat tttacggata tgttttttta
     9181 agcctgaagg cataggttga atggaaacat aaaggtaatt ataaatatgg caacaaatca
     9241 taatttgcta caagaaaata atcggcgtaa atgcctgttt acgccgatta tgtatttaca
     9301 tagtgagttt tatgccagca aagaacgtgc gaggaagtga aggcccgtac atgtatccgg
     9361 catcacgaag cggtccccgg tcgaaatctt tttgaaaact gttgagtata ttttgaacac
     9421 ccccgttgag ttgccaggtt gttttgtccg ttgcaaaaaa gttataagcg agtttaatcg
     9481 tggaatcaaa aaaaccgggt gtgcgaaatt cggtatcttc cggtatgtaa ccggcaaaat
     9541 gttgtaccaa catgggacct gtgtatacac cggatagcga aatgttgaat gcttcagtcg
     9601 gtgtacaggt tgctgtgaaa tagccgtaga tatcgggagt gcgaaacatc ttgcgttgcg
     9661 ggtcgacgtt tggattgttg ctccacgatt ccggtttgtt atacatgctt tcctggattg
     9721 ttccgcccat ctgtaattga aactttcggc ccggagcaat ttttccttca atgtttacac
     9781 ctttcacgac ggctcccgac ccgttccgcc tttcctttaa caggttgccc tgcggatctt
     9841 ttccaatctc ttccaacaga aatacgtctt tcagatgtgt ataaaagcct tcgatcagta
     9901 aattcgtttc tacagggccg aacgctttgt acaaatcggc agaaatactg actgtttgtg
     9961 agtattcagg tttcagacca ggcgatagct gaatgatgct tacttctccg cccacagcgg
    10021 taatgtgaag gtcttcgtcg aaggcttgtg gggcgcgaaa cccggtggaa taaccgccac
    10081 ggaggacaac aaggggggta ggagcgtaac gtatgttaat tctcggactc agaatagctt
    10141 ttttgatcag gttgtgctta tccagtctca ctcctgttaa aatactccaa gccgcatttt
    10201 tccattcgtt ttgcaggaaa gtgctttttg tgttcacctc ttggtccaac tttcggttat
    10261 aacccagcat ttcatcactc atcgtattgt aattcagttc tactcctgtt gtaactacag
    10321 acggcataaa caaaaacctt tccgaggaat aactgtattg tactcctccc atcatactgt
    10381 tgtcagcggt tgttccgtag gcgttcggat ctctttgggt cccgtaatag ctttgacggt
    10441 agataaattg cccggacagg taagcattta ttttgtgctt gtaatttcgc gaaagaaagt
    10501 tgtaattggt gtttgccgaa tgaatgttgt gttccgtttg ttcggtaatg tcggtagtat
    10561 gaggaggttt gtcgagcatg ttccctccgc gccggaattc attgataaaa tgatactcta
    10621 tagttagttt gttgtaattg ttaaacttgt agaatccgcg gaaccccatg gtgttgcctc
    10681 gcagtttgcc tatctccgtg aagccgtcgt cgtcgtaatc caatgcttcg cgttggcgat
    10741 tggatgcaaa aacaagcagg ccggtcttta ggctgttgtc caccagcgaa gcgtggaacg
    10801 ataggttgtt gtcgggagaa gatgcaccga taatgtttgt tacgttactc acggaaaggt
    10861 tgtttgtttt gggttctttt gtgataatat tgatggtccc tgctatggca ttggacccga
    10921 aaagggcgga tcctccacct cgcatcacct ccacgcgctc tatcatgttg gccggtattt
    10981 gttcgatacc atatacgcca gccagagcgc tgaatatcgg acggctatcg atgagaattt
    11041 gggagtaggg gccgtcgagc ccattgatgc gaacttgttg aaatccgcag ttctggcagt
    11101 tgctttcaat ccgtagtccc ggctgaaaat tcagtccttg tgccagggtc accgaattgg
    11161 tgctctccat cagcaagggg gttatcacgc tgaccaccga aggagcttct ttcctttttg
    11221 tttctgtccg gtttgccgaa acaacgacgg tttcaagcaa tacctcatca tcttccatta
    11281 caaagttcac ttcgatggtt ttcccgccct ccagggttac ggggctctct acagatttat
    11341 atcctacccc agacgcaaca agtatgaagt tgcctttggg aagatccttc aggtagtaat
    11401 gtcctgtttc atcggtggcg gtggcatgcg aagtttcttt taaagaaacg gttacgaacg
    11461 ggatatgttc ttttgtagac tgatgtaata tgtggccgac tatgttagcg tcgcttcttc
    11521 catgctggtg cctgtgttgc atacgcacct gatttttttg ttggggaaaa accgcaagag
    11581 tatatacaat tgcgaacaaa acccatataa gtttcttcat ttcaattttt taattaatta
    11641 cacgtgtttg cctggacatg cacatgagtt gtgctgcccg tgttgtggcc aagaaaaaaa
    11701 atattcttac cgaaaatcta tgtgtgtaat tgaattggag gcgcacgcca cccatttgcc
    11761 cggatgagca cgcgttttag ggcgaaatgt tcttgcttgg gacggatata gccgcttatc
    11821 tgcttgctta gtgcagcata tgcagcacac aggaacaagc ccgtgaaata gaaatgcgag
    11881 agtgcctgga tgaggttaag tgaggaagta ccgtgctggt gatcggtttt gtttccgtct
    11941 tgagccaaaa aattaaagtg ggaatggaca atgcgttcgc cgttaacttc atgggtatga
    12001 ataaataaag aaatatttcc gtaataactt acgaaaagca ccagcagaaa atatcccgat
    12061 agtatgctga accgttgtct cattgctata acaaagatac ggctatccgg aattctgttt
    12121 aaacagtatt gataaaattg attattcggg aaaagctata atacagtcaa gtgaagttat
    12181 tcctccctta tgatattctc aatctccttc attaccagcg gtaatgctgg atataccatg
    12241 ccgtgatcgt agccgtcgag ttcgagaagg cgggttttct tgtgtcccgc cactttcatc
    12301 attcgcgcaa gatatgcgtt ttcttcgtac ctgccaagca tctccaattc tctgtctccg
    12361 gtaatgagca ggagtggcgg agcatcgtga cggacgtgaa aaagaggagc aaattcatcc
    12421 acaacgggtt gtgtgttttt tattccccgc tgttcgcgga tattaaagtg tgtgatggtt
    12481 tgtgcgctga ggggaataag ccctgcaatg cggttggcat caatattgtg cgctgccagg
    12541 tattttttgt ctaaacccgc catacaggta aggtaaccgc cggcagaatg tcccgatacg
    12601 aaaattttgg atgcgtcacc accgtatttt tcgatgtttt tgaatgccca ggctattgca
    12661 gcagctgcat cttctatata ggcgggtgcc ttaacttttg gtgagagccg gtaattaacg
    12721 ccgatgatgc aggtattttt atgtttgaac ccgtcgggaa gctctttctc tccgcctgtt
    12781 aatccgccac cgtgaaacca aacgacggtg gcaaaatctt ttttgtcggt tggatagtaa
    12841 atatcgagaa tacaacgttc cctgatgtat gcatctgact ccatcttcgc atcatcgtaa
    12901 taggggatat tcctgatgat tctgtagttc gtttcctgcg caataccact cagtgaagtg
    12961 ctgattaaca aagagataag aagactcttt ttcattacca attatacatt atgaattgtc
    13021 aattataaat attatcttga ttagaacacc cattcttcgt tttggggaga tgacgtttgg
    13081 gatttacctg ttccgaaact cagtagccgg ccttttaaat cgaaagacag ccatcgatcg
    13141 gaaagttgca gttctgaacg gtgggtgtag aaaacctctc aacaatcatt ccgggttgca
    13201 attgattcca tctttcactg aaacgattgt tcgccaaggc tgtagtcctc cgtatcatat
    13261 cgtttgcatg gtctaggtaa ttgtttttgg cttcttcgtt ttggaatatt tttatcacgt
    13321 atgtacgttc gccttcatcc agaagataat tggattgaaa cagctgcaca taatttttgt
    13381 accggtacct gttgagtatc tcacggataa aagctttgaa ttcccgttca acatccgctt
    13441 ccgaagaatt tacatcccac cggatggttt gataagccac gtcattcttc atttttacga
    13501 ccgtttttcc accgaaagtc aacgtctcgc aactgcttaa cgaaataagc atcacacctg
    13561 aaatgatgag tttaagaatc gttgttttca tctgcctgtt tctttagttt acgttaaaaa
    13621 gcaccttttt tagagtgtgc aagtgcgaga atggttataa aaaagctaaa aatccggtaa
    13681 tcatcagcac gttgatgaaa tcgataaaga acgctcccac tacgggaaca ataaaatagg
    13741 ccacacgaga atagccgaac ttatcgcaaa tggaatccag gttcgccatt gcgttggcgg
    13801 tggaacccat tgcaaaaccg atcagtcccg atgtggaaac cgccgaatcg taatttcgcc
    13861 ccatagccgg aaaggctacg aaccggggga aaacatacat caacattacc tgtgcgagca
    13921 acaaaaccat cagcggtaat gccagactgc tcaattccca gaggcgcatt gtcataatgg
    13981 ctatggatat gaaaatgttc aggcagatat cgccaatcag ttggatttcg gtgttgagcg
    14041 taaaatcttc cctagtatcc ataatgttac ggataatggc tgccacgatc attggtccga
    14101 tataaatagg aaattttacc ataccgataa acattcccat aaaattattc agtaaatcgg
    14161 taaaaattac gccgataccc atcgaaacaa ggataaggaa cgatgctttg gtaattcgtt
    14221 ctccgtccat tgggaccggg tgagcttgca tcgcatcgat tttctgttct tcttcatcgg
    14281 cttttcgagt gctcagcagc ttctttcgca caatcaggtt atttccaatg ggagcaccca
    14341 tcagtgaccc catcagcaaa ccgaaagtgg cggatgctat tgccacggcg tgagcacccg
    14401 aaatacctgt agcttccact tcgggggcaa tggccgccga agtgcctagc ccgcccatca
    14461 gtgcggtaga accggtcatc atcgccagtt gaggcgagag attgagcggt tttgccaacc
    14521 caatagcaac aaggttttgc aacaggctga aaatcgtggc caatccgaga aagataagca
    14581 aaaccgtacc gccttttttc agca
//
LOCUS       DAOSDR010000020        14524 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrimonas sp. isolate AMR_MDS_1826
            NODE_1649_length_14524_cov_3.700256, whole genome shotgun sequence.
ACCESSION   DAOSDR010000020 DAOSDR010000000
VERSION     DAOSDR010000020.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863355
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrimonas sp. (wastewater metagenome)
  ORGANISM  Petrimonas sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Dysgonomonadaceae; Petrimonas.
REFERENCE   1  (bases 1 to 14524)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14524)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:10:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,720
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 35
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14524
                     /organism="Petrimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1649_length_14524_cov_3.700256"
                     /isolate="AMR_MDS_1826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2023866"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            455..1540
                     /locus_tag="PLI30_01555"
     CDS             455..1540
                     /locus_tag="PLI30_01555"
                     /inference="COORDINATES: protein motif:HMM:TIGR03718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC/Alx family metal homeostasis membrane
                     protein"
                     /protein_id="HOI78269.1"
                     /translation="MNAIALYWIAFIAIFIIVYGIDLYVTSHRQRTISVKSSLTWTAV
                     WISVAIAFGASLYFLFPQNPESTVRTGPVMMTKFISGYLTEYSLSVDNLFVFILIFSM
                     MGIHPKNQPKLLKLGILISVVLRILFILVGMELVERFHWIIYIFGVVLIWTAYKMAFS
                     KEDENVNPKSNILYKGASRLFPIDPDIHSPHFFTQINGKIHLTNIFLALLVIGSTDIL
                     FAVDSIPAIIGVIKEGINNVLTLSEENFLAISSNVFAVMGLISLFFALKGIMGMFRYL
                     KTGVSFILLFIGMKMLLSSVHSVGEFFAHHSWVSLAVIIGTLIISILLSMLISERKES
                     DKLQEAVQKLKEEMEESKPKHVGKFMH"
     gene            1630..3051
                     /gene="hydG"
                     /locus_tag="PLI30_01560"
     CDS             1630..3051
                     /gene="hydG"
                     /locus_tag="PLI30_01560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019541400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[FeFe] hydrogenase H-cluster radical SAM
                     maturase HydG"
                     /protein_id="HOI78270.1"
                     /translation="MKFTPEKYAIPDAPMQPFIDTGEIWDFLNKTISTPVKVKEVVEK
                     SLAKSRLNLEEVAILINATDPGSVQLIKEGAKELKKKIYGNRIVLFAPLYVGNKCSNN
                     CAYCGFKASNRDAVRKTLSDKELVKEIEVLEDNGQKRLILVYGEHAQYSPEFIAQTVK
                     IAYSVKKGNGEIRRVNINAAPLDIEGFRTVKEAGIGTYQVFQETYHREAYKTYHLRGK
                     KADFDYRLTSLDRALEAGLDDVGIGALFGLYDWRFEVMGLVRHTNHLEACYNVGPHTI
                     SFPRIKDTSMLDLGDEYFVSDEDFTRLVAILRLAVPYTGMILTAREPAELRNQLIQFG
                     VSQIDGGTKIELGSYAEKEQNHDLKKGQFRINDDRSLNEIIDELLQQDMLPSFCTACY
                     RLGRTGEHFMEFSVPGFIKRFCTPNAILTLAEYLMDYAPEHTAEKGWDVIEKNIHELN
                     ENVQHQVRERIEKIKRGERDLYF"
     gene            3060..4241
                     /gene="hydF"
                     /locus_tag="PLI30_01565"
     CDS             3060..4241
                     /gene="hydF"
                     /locus_tag="PLI30_01565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018336279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[FeFe] hydrogenase H-cluster maturation GTPase
                     HydF"
                     /protein_id="HOI78271.1"
                     /translation="MTNSRPHIGIFGRRNTGKSSLINLLTGQETAIISDIPGTTTDPV
                     KKSVEIFEIGPVVLVDTAGIDDAGELGRKRINKSLDAIKKVDCAVLLIAGNQFGQYEL
                     ELIRHFGKFGIPYLILHNKSDLSKIAALTKTAIKHHSDASVLDFSAKDPGDSEKIIDA
                     LKKIIPGESQQHRTLIGDLVKPKDTVLFITPIDSEAPEGRLILPQNQAIRDALDNHCI
                     TVVVKETELDDFLKLGIPPALVVTDSQAFGYVATRIPENVPLTSFSILFARQKGDFND
                     YLQGTPHLSKLKDGDNILMLESCTHQTNCDDIGRVKIPQLLRNFTGKNLSFTVVGGLS
                     EIAPIDYSLVIQCGGCMVTRKQLINRIKPFTEKDIPVTNYGMVLAYVNGIFERATRMF
                     L"
     gene            4238..5356
                     /gene="hydE"
                     /locus_tag="PLI30_01570"
     CDS             4238..5356
                     /gene="hydE"
                     /locus_tag="PLI30_01570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018338515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[FeFe] hydrogenase H-cluster radical SAM
                     maturase HydE"
                     /protein_id="HOI78272.1"
                     /translation="MIPTLLHKQKLLQEDLVRLLCTEGEEKQLLFDYAAKIKQENVGN
                     TVYLRGLIEFSNICEKNCYYCGIRRDNTHTHRYCLSCDEALEAIRFAYENGLGSVAIQ
                     SGELTSDTFTRKIDSLLKEAMRITNREIGITLSCGEQPEEVYRRWFESGASRYLLRIE
                     SSSGELYRKIHPDDDTHRFDKRLNALESLKKIGYQTGTGVMIGLPFQTVEDLANDLLF
                     MKQFDIDMCGMGPYIEHAQTPLYKNKSELLSLRERFNLSLKMVALLRILMPDINIAAT
                     TALQSIEKEGREKAILIGANVLMPNITPKQYRDDYSLYQNKPVSQQSNEENLLFLEQQ
                     LKSIGHEIGYFRQGNSKHYKTHQISSNRAVKNLNCLGS"
     gene            complement(5305..6684)
                     /locus_tag="PLI30_01575"
     CDS             complement(5305..6684)
                     /locus_tag="PLI30_01575"
                     /EC_number="4.1.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019538663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050371 - tyrosine phenol-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006570 - tyrosine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine phenol-lyase"
                     /protein_id="HOI78273.1"
                     /translation="MNLNDYPAEPFRIKTVETVKMISREEREKVIQEAGYNTFLINSE
                     DVYIDLLTDSGTNAMSDRQWAGMMMGDEAYAGSKNFHHLEQVVQEIFGFKHLVPTHQG
                     RGAENLLSQIAIKPGQFVPGNMYFTTTRYHQERNGGIFVDIIRDEAHDAGLDIPFKGD
                     IDLNKLQKLIDEKGAENIAYVCLAVTVNLAGGQPVSMKNMREVRQLADKYKINVFYDA
                     TRCVENAYFIKEREEGFAGKSIQEIVREMFSYADGCTMSGKKDCLVNIGGFLCMNDED
                     LFAAAKELVVVYEGMPSYGGMAGRDMEAMAIGLQESTQVEYIEHRIKQVRYLGDKLKA
                     AGIPIIEPVGGHAVFLDARRFCPHLTQDQFPAQSLAANLYVESGVRSMERGIVSAGRD
                     KNTGKNHAPKLETVRLTIPRRVYTYRHMDVVADAVINLYKHKEKIKGLRFVYEPRQLR
                     FFTARFEEI"
     gene            complement(6875..7519)
                     /locus_tag="PLI30_01580"
     CDS             complement(6875..7519)
                     /locus_tag="PLI30_01580"
                     /inference="COORDINATES: protein motif:HMM:TIGR04085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOI78274.1"
                     /translation="MRNNPRSFDRAVAAIDLAVRSLRLNFDVVTCVNKKNISELSLIK
                     EFLKSHKVKAWRLFTIIPIGRAVQNEELSLSDEEFVYLMDFIRELRENEDDIDVKFSC
                     EGFVGKYEWKVRESFYFCRAGINIGSILIDGSISACPNIDRSFSQGNIYTDNFNDVWE
                     QRFNSFRDRNWMRVGQCKDCRRFKDCQGNGFHNWHGSKQNVLVCHRSKIENAHY"
     gene            complement(7532..7939)
                     /locus_tag="PLI30_01585"
     CDS             complement(7532..7939)
                     /locus_tag="PLI30_01585"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOI78275.1"
                     /translation="MIPSVKRALFRKFKAVETRRHELSYLFWECTTRCNLDCLHCGSD
                     CSKQSNYRDMPMEDFLKALDTIKNPAKNLMVVVTGGEPLLRKDLPACGREIRRRGMRW
                     SMVSNGYFYDEAVHNSLLNAGLGALTISLDGLP"
     gene            complement(7973..8296)
                     /locus_tag="PLI30_01590"
     CDS             complement(7973..8296)
                     /locus_tag="PLI30_01590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI78276.1"
                     /translation="MRQNWIRNIVGGLSFTTALFIFQACYGTPQDFGLDVFVEGKVVS
                     KTTGEPIKGIKVSAASLQHTVTDDKGMFSFYTVRSASNQFRRCGWSRQRNIYQERQPR
                     LYRER"
     gene            complement(8399..9076)
                     /gene="trmD"
                     /locus_tag="PLI30_01595"
     CDS             complement(8399..9076)
                     /gene="trmD"
                     /locus_tag="PLI30_01595"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015531117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N(1))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HOI78277.1"
                     /translation="MRIDIITVLPEMIEGALNCSILKRAQDKGLVQFGLHNLRDYTLD
                     KHRRVDDYPFGGEAGMVLQIEPIDRCISHLKSQRDYDEVIFTTPDGEQFSQKTANELS
                     LLENLIILCGHYKGVDYRVREHLVTREISIGDFVLTGGELAAAMIADAVVRVIPGAIG
                     DEQSALSDSFQDNLLAPPVYTRPAEYKGWRVPDVLLSGHERKIAEWRLEQAHERTKRL
                     RPDLLGR"
     gene            complement(9087..11552)
                     /locus_tag="PLI30_01600"
     CDS             complement(9087..11552)
                     /locus_tag="PLI30_01600"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOI78278.1"
                     /translation="MKQTLLSLILLLTSVVVYAEGTNVKGQLVSANDKQPLPYATVSV
                     ASKAVPQKSIKKFATDDSGNFTTTLQAGDYIFTFQFVGMNNFNRSVEVKNGELQVNLG
                     QIEMSESSTELSEISVTAQRPLVKVEIDKLTYSAKDDPESSTSNVLDLLRKVPLVTVD
                     GEDNIQLKGSSSFKIYLNGKPSNMISNNPSQVLKSMPANSIKDVEVITDPGARYDAEG
                     VGGIINIITDKRVDEGYSGSVGANGDTFGGYGGNAYLSLKYGKFGLTGNGSYFYHNRP
                     EAKTSLTREEMAPSPVNELTQDGTSKNYGGGLFYNTQLSYEPDTLNLFNVSVGQFGGK
                     FHSNSSQDVLSQGARDYSYLLNNNSINHFGSFTLTTDYQRTFKKKGELLTVSYRFERN
                     PNDSEYESEYSQVVNYFYPKGYRMKSINNAGGKEHTGQVDYVNPLTDKHSIEVGLKYI
                     FRDNSSRGDNTFLNPDDGGQWEEDKSRKNDLDHEQRIASGYAGYGLKAGKFGLKLGLR
                     GENTAQKIHFMSVQTDTVVHSDFFDMVPSAAFSYQLGMTKTLRWGYNMRISRPGIWYL
                     NPYINDSDPNNIRYGNPNLDAEQTHNLNINYGSFSQKVNFNATLSYSFTQNAITSRIF
                     IDEYQGREGVTHNTYDNIGRNQSIGLDGYLSWTPTPVIRMNVNGSLSYTDIQSTENAG
                     VRNSGFSGRGFGGITFTLPKDVRLSANGGIFTSNIQLQTTQSAFYFYSFSAMKSFFNK
                     KLDVSLNTTTPFHKYRDFKLTTTGEGFVQKMNFQNPMRTFRLSLTYRFGDLKSSVRKV
                     QRTITNEDVLQGGSSQEGTGTGS"
     gene            11807..12088
                     /locus_tag="PLI30_01605"
     CDS             11807..12088
                     /locus_tag="PLI30_01605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006800018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015661 - L-lysine efflux transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:1903401 - L-lysine transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysO family transporter"
                     /protein_id="HOI78279.1"
                     /translation="MLNIILIILSGIVVGYFARKLPQVKHVGSVISLIIMLLLFFLGV
                     SVGANEQVVTNFSTIGLDALIISFGATVGTVLCAWLVYSTFFRRKGKNS"
     gene            12085..12687
                     /locus_tag="PLI30_01610"
     CDS             12085..12687
                     /locus_tag="PLI30_01610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007655883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine exporter LysO family protein"
                     /protein_id="HOI78280.1"
                     /translation="MKQSLIYLLLFALGVILALWGHIPDVLLKDDISKWILYGLLFFV
                     GVQIGSGKNMFTAVKRFGFKIALVPVATTVGTFAGAALTSLLLPERSLIDCLSVGAGF
                     AYYSLSSILISEFRGAELGTVALLANIMREFIVLVFTPWLVKYFGKLSPICAGGATTM
                     DTTLPMITKYSGSDYVVVALFHGMVIDFSVPLWVSFFLTL"
     gene            12694..13806
                     /locus_tag="PLI30_01615"
     CDS             12694..13806
                     /locus_tag="PLI30_01615"
                     /inference="COORDINATES: protein motif:HMM:NF024258.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HOI78281.1"
                     /translation="MKSFFLTILFFYVTANVYAAIRIYQLIPANAWVRIVVMGIFLLG
                     FASLLIFFRFGEAMPVGTAGFFYRFGTSWMIAFLYVFMLILFVDLFRIINWVAPIADK
                     ETVRGIFHHNALTAVTGLGLTALILLYGNWQYHNKKRSHITIKTEKIDKPMRIVGISD
                     LHLGYTISKKELSRWVEMINAENPDMVIIGGDLVDNQLRPVWMHSLDRELLKIKAPLG
                     VYACTGNHEYISGIKNSADFYTRSGITLLRDSVLQTNGLTIIGREDHSRKNRKTLPEL
                     IKNSDNRTFSILLNHQPYDLDEAVREGIDFQFSGHTHRGQVFPASLITDKIFELSQGY
                     IQKKNTHFYVSSGLGIWGGKFRIGTRSEYLVLDLEN"
     gene            13822..>14524
                     /locus_tag="PLI30_01620"
     CDS             13822..>14524
                     /locus_tag="PLI30_01620"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HOI78282.1"
                     /translation="MRSLIVALIIHFTLNILVFLKGWDVFKTKKLLRTFWMVIFAFEL
                     LVYLTGFAFYRHLPPEIIHPIRMMGTSWMLFLLYLGGLVLIGDFLYLASRKKLTRPKE
                     LLNQPAKMKLTLFLSSFAVVILTLSYGNYKFNHPEVRQVDIQVGKSAGKMDSVRIAMV
                     GDLHLGYLINRDDAQRMVDLIMEQEPDLILFVGDILDSSIEPVQGQRMDEELRRLQAP
                     LGVYSCTGNHEYRYEA"
BASE COUNT         3861 a         3445 c         3226 g         3992 t
ORIGIN      
        1 aacgtccgca agacctcccg tggtaaggca attaactttt atcccatatc cccgccgcat
       61 ttacatccct gtgtccgtat agtctttgga ttttgacttg aaatgcagtc ttatcccaca
      121 ggttatgcct gtatacgatt cgtgttactc ggggcaggac tttgccgtgg gcttccttca
      181 gattccacct cacgatggac acccttgccg ttggctaatg gttggcgact ataaacctcc
      241 ataatggact ttcaccatcg agttaatcac catgcacggc gcacataaaa aagccagaag
      301 cgaagatgct cctggctttt aacttaattt aaaccggatt aagaataacg gatggaaatt
      361 cagtgtaatt ttagctgatt tttatccggc agaacatcta ccctttaaaa tcgtagagac
      421 cattaaaaaa caacggtaat tctcttcttt ttttatgaat gcaatagcac tttactggat
      481 tgcttttata gccatcttca tcattgtgta tggaatagac ctctacgtga cttcccaccg
      541 ccaaagaaca attagtgtga aatcatcctt aacctggaca gccgtatgga tttccgtagc
      601 aatagcattt ggagcttctc tttattttct cttccctcag aatccggaaa gcacggtgcg
      661 aaccggtcct gtaatgatga caaaatttat ctcggggtat ttgacggaat attcgttatc
      721 cgtggataat cttttcgtat ttatactgat cttttcaatg atgggcattc atccgaaaaa
      781 ccaaccaaag ttactgaaac tgggaatttt gatttcggtt gtactaagaa ttctttttat
      841 actggtggga atggaactgg tagaacgctt tcactggatt atctacatat ttggtgttgt
      901 tttgatttgg acggcataca agatggcttt ttcgaaagaa gatgaaaacg taaatcccaa
      961 atcgaacatt ctgtacaagg gagcttccag gttattcccc atcgatcccg acatacattc
     1021 gcctcatttt ttcactcaaa taaacggaaa aatccactta accaatattt ttcttgcctt
     1081 gcttgttatc ggctccaccg acatcttgtt tgctgttgac tctattccgg ctattatcgg
     1141 cgttataaaa gaaggtataa acaacgtcct tacactcagt gaagagaact ttcttgccat
     1201 ttcttccaat gttttcgcag tgatgggctt aatatcgctt tttttcgcgt tgaaaggaat
     1261 tatgggaatg ttccgttact tgaaaacagg tgtgagtttc attctcttgt ttataggcat
     1321 gaaaatgctg ctgagttccg tgcattcggt aggagaattt tttgcacacc attcatgggt
     1381 ttcgctcgcg gttatcatcg gcacgttgat tatttctatt cttttatcca tgctgatttc
     1441 cgaacgaaaa gaatccgata agcttcagga agctgtgcag aaattgaaag aagaaatgga
     1501 ggagtcgaaa cccaaacatg tgggaaaatt tatgcattag ttgattttcc agtacagaac
     1561 acgagaaagt tctgtgattg atggaataaa ttttgtaatt ttgcagttca ccttgtgaat
     1621 aaacatttta tgaaatttac cccggaaaaa tacgccattc ccgatgcacc gatgcaaccc
     1681 ttcatcgata caggcgagat ctgggatttc ctcaacaaaa caatctccac acccgtcaag
     1741 gtgaaagaag tggttgaaaa atcactcgca aaaagcaggc tgaatctcga agaagtggcc
     1801 atacttatca atgctaccga tccggggtcg gtacaactca ttaaggaggg agccaaggaa
     1861 cttaagaaaa aaatttacgg gaaccgaatc gtgctgttcg cacctcttta tgtgggcaat
     1921 aagtgctcca acaattgtgc ttactgcgga tttaaggctt ctaacaggga tgccgttcgt
     1981 aaaacgctct cagacaaaga actggtaaaa gagatagaag tactggaaga taatggacaa
     2041 aagcgattga ttctcgttta cggcgaacat gcgcaataca gccctgaatt cattgcccaa
     2101 actgtaaaaa tcgcatacag cgtgaagaaa ggcaacggcg aaatccgccg tgtaaacatt
     2161 aacgcggccc cactagacat agaagggttc cgtacggtga aagaagccgg tatcggcacc
     2221 tatcaggtgt ttcaggaaac ctatcaccgc gaagcataca agacctatca tttacgcggt
     2281 aaaaaggctg atttcgacta ccgcctgact tcactcgacc gggcgttgga agccggactg
     2341 gacgatgtgg ggataggtgc acttttcgga ttgtacgact ggaggttcga ggtgatgggg
     2401 ctggttcgcc acaccaatca tctggaggca tgctacaacg tagggccaca caccatttcg
     2461 ttcccccgga tcaaagacac atcaatgctt gatttgggcg atgaatattt cgtgtcggac
     2521 gaagatttta cccggttggt agctatactc cggttggctg ttccatacac gggaatgatt
     2581 cttacagccc gcgaacccgc ggagcttcgt aaccaactga ttcagttcgg ggtatcgcaa
     2641 atcgacggtg gaacaaaaat tgaattgggc agttacgctg aaaaggagca aaaccatgat
     2701 ctcaaaaaag ggcaattccg tataaacgac gaccgatcac tcaacgaaat catcgacgag
     2761 ttgcttcagc aggatatgct accgtccttc tgtacggcct gctaccgtct gggacgtacg
     2821 ggtgaacact ttatggagtt ctccgttcct ggcttcatca aacggttctg cactcccaac
     2881 gcaatactca cgctggccga atacctgatg gactatgcac cggagcacac cgcagaaaag
     2941 ggatgggacg tgattgaaaa aaacattcac gaactcaacg aaaatgtaca acatcaggta
     3001 cgtgaaagaa tagaaaaaat aaaacgggga gaacgggatt tgtattttta attggggaaa
     3061 tgaccaattc cagaccacat atcggcattt ttggccgccg gaacaccggt aaaagctcac
     3121 tgatcaacct gctcacgggg caggagaccg ctataatatc cgacataccc ggcacgacga
     3181 ccgacccggt aaagaaatcc gttgagattt ttgaaatcgg acctgtagta ttggtggata
     3241 ctgccggaat tgatgatgcg ggagagttgg gaagaaaacg catcaacaag tcactcgatg
     3301 cgataaagaa agtggactgc gccgtgttac tgattgcggg aaatcagttt ggacagtacg
     3361 aactggagct tattcggcac tttgggaaat tcggtatccc ctacctcatc cttcacaaca
     3421 aaagcgacct gtcaaaaatt gctgccctca caaaaaccgc aataaaacac cacagcgatg
     3481 cctcggttct cgatttcagt gcaaaagatc cgggagattc ggagaaaata attgatgcac
     3541 taaagaaaat aatacccggg gaaagccaac aacacagaac gcttataggc gatctggtaa
     3601 aaccgaagga cacagtattg ttcataactc ccattgacag cgaagctccc gaaggacgac
     3661 tgatcctgcc gcaaaaccag gccatacgcg atgctctgga taaccattgc attacagtag
     3721 tggtaaaaga gaccgagttg gacgattttc tgaagctagg tatacctcca gcgcttgttg
     3781 ttaccgacag tcaggccttt ggctatgtgg caacacgcat tcccgaaaac gttccgttaa
     3841 caagcttcag catcctgttt gcccgacaaa aaggcgattt caatgactac ctgcaaggca
     3901 ctcctcacct gtctaaacta aaagacgggg ataatatcct tatgttggaa agctgcactc
     3961 atcaaaccaa ctgcgacgat atcggacggg tgaagatacc gcaactcctc cggaacttca
     4021 ccggtaaaaa cctatcgttc accgttgtag gcggattgtc ggagattgca cccatcgatt
     4081 attctctcgt catccaatgt gggggatgca tggttacacg caaacaactg ataaaccgga
     4141 taaaaccgtt cacggaaaag gacattccag tcaccaatta cggaatggtc ctcgcgtatg
     4201 taaacggtat ttttgaacgg gcaacgagaa tgtttttatg attccaaccc tattgcataa
     4261 acagaaactt ctccaagaag atttagtccg cctactctgc accgagggtg aggaaaagca
     4321 acttttgttt gattatgccg ctaaaatcaa acaagagaac gtgggcaaca ccgtttatct
     4381 gcgcgggctg atcgaatttt ccaacatttg cgaaaagaat tgttattatt gcggtatccg
     4441 ccgggataac acgcacacac atcggtactg tttgtcctgc gatgaggcat tggaagctat
     4501 ccggtttgcc tacgaaaacg gattgggatc cgtcgccatc caaagcggag aactcacgtc
     4561 ggatacattt acccgaaaga tcgactcctt actgaaagaa gccatgcgta ttaccaaccg
     4621 ggaaatcggc attaccctct cttgcggcga acagcctgaa gaggtctatc gccggtggtt
     4681 tgagagcgga gccagccgct atttgctccg tatcgaatcc tcctccggag aactttaccg
     4741 taaaatacat cccgacgatg atacacaccg ttttgacaaa cgcctgaatg ccctggaatc
     4801 gctaaaaaaa ataggttacc aaacaggaac aggcgtcatg atcggcctac cgtttcagac
     4861 tgtcgaagac ctggcaaacg atttgttgtt tatgaagcaa ttcgacatcg atatgtgtgg
     4921 tatgggcccc tacatcgaac atgcacagac tccactctac aaaaacaaga gtgaactgct
     4981 ttcgctgcgg gaacgattca atttaagcct taaaatggta gccctcctgc gtatcctcat
     5041 gcccgatatc aacatcgccg cgaccaccgc cttgcaatcc attgaaaagg aaggccgcga
     5101 aaaagccatc ctcatcggcg ctaatgtact gatgcccaac atcactccga aacagtaccg
     5161 ggatgattat tccctttatc agaacaagcc cgtctcgcaa cagagtaacg aggagaacct
     5221 gctttttctc gaacaacagc ttaaaagcat aggccacgag attggctatt tcaggcaggg
     5281 aaattccaag cattacaaaa cgcatcagat ctcctcaaac cgtgccgtaa agaacctcaa
     5341 ctgcctgggt tcataaacaa aacgaagccc cttgattttt tccttgtgtt tatacaggtt
     5401 gataaccgca tcggctacca catccatgtg acgataggta tacacacggc gtggaatggt
     5461 taaccgaacc gtttccaact tgggcgcatg gtttttgccc gtgtttttat cgcgtccggc
     5521 agaaacgatg cctcgctcca tgcttcgaac gcccgattct acgtagaggt ttgccgccag
     5581 gctttgcgcc gggaattgat cttgcgtgag gtgcgggcag aaacggcgcg catccaggaa
     5641 aacggcgtgg cccccaacag gctctataat aggaattccg gccgctttga gtttatcacc
     5701 caaataacgg acctgcttga tgcggtgctc tatatattca acttgcgtag attcctgcaa
     5761 tccgatagcc atggcttcca tatcgcgtcc ggccattccg ccgtatgaag gcattccctc
     5821 gtaaaccacc accagttctt ttgcagcggc aaacaggtcc tcatcgttca tgcacagaaa
     5881 accgccgata ttcaccagac agtccttctt tccgctcatg gtgcaaccat cggcataact
     5941 aaacatttcg cggacgattt cctggatcga tttaccggca aagccctctt cccgttcttt
     6001 gatgaaatag gcattctcca cgcatcgggt ggcatcgtaa aacacgttaa tcttgtattt
     6061 atcggccagt tgcctcacct cccgcatgtt tttcatggaa acaggctgcc cgcccgccag
     6121 attcacggta acggcgaggc agacataggc aatgttctca gccccttttt cgtcgataag
     6181 tttttgcagt ttgttcaggt cgatgtctcc tttaaacgga atgtccaatc cggcgtcgtg
     6241 cgcctcatcg cggatgatat cgacaaaaat tccgccgttg cgctcctggt gataacgcgt
     6301 ggtggtgaaa tacatgtttc cgggcacgaa ttgcccgggt ttaatggcaa tttgcgaaag
     6361 caggttctca gccccgcgcc cctgatgcgt aggcaccagg tgtttgaatc cgaaaatttc
     6421 ctgcacgact tgttccagat gatgaaagtt cttgcttccg gcataggctt cgtcgcccat
     6481 catcataccg gcccactgcc tgtcgctcat ggcattggtt ccgctatcgg tcagtaaatc
     6541 aatatacaca tcttcggagt tgatcaggaa agtgttgtat ccggcttcct ggataacttt
     6601 ttcccgttct tcacgggaga tcattttaac ggtttccacc gtttttatgc ggaaaggctc
     6661 cgcaggatag tcgtttaaat tcataatagt ggtattaatg tgtattggca ttatacccca
     6721 aaattatgac aaaaagacga aatgacaaaa gaatttaagt gtatcttttg aaaataacta
     6781 acaaatagaa ataatcgagt ttctaaatga caaaagaaat gtaaaaatta aaactcctaa
     6841 taaccaacaa aatgacgact ttcagttcaa agaattaata atgcgcgttt tctattttcg
     6901 accgatggca aaccaataca ttttgtttac tgccgtgcca gttatgaaaa ccgtttccct
     6961 ggcaatcctt gaaacgcctg caatctttgc attgacccac gcgcatccag ttcctgtcgc
     7021 gaaaagagtt gaaacgttgt tcccatacgt cgttgaaatt atcggtgtaa atgtttccct
     7081 gtgaaaaaga gcgatcgata ttgggacaag ccgatatgga gccgtcaatg aggatagagc
     7141 cgatattgat acccgccctg cagaaataga aactctcccg gactttccat tcgtatttcc
     7201 ccacaaatcc ttcgcaactg aacttcacat cgatatcgtc ctcattctcc ctcaactccc
     7261 gaataaaatc catcaaataa acaaattcct catccgagag cgacaactcc tcgttttgga
     7321 ccgcccgtcc tatcggaata atggtaaata gccgccatgc cttcaccttg tggcttttaa
     7381 gaaactcctt gattagggaa agttccgaaa tatttttttt gttgacacac gtgaccacat
     7441 cgaagtttaa ccggagcgaa cggactgcca ggtcgattgc cgcgaccgcc ctgtcgaaac
     7501 ttcgcggatt gtttcgcaac cagttgtgtg actacggcaa cccgtccaag ctgatggtaa
     7561 gcgctcccaa accggcgttg agtaacgaat tatgtaccgc ttcatcgtaa aaatacccgt
     7621 tagaaaccat ggaccagcgc attccgcgcc ggcggatttc acgtccgcac gcaggcagat
     7681 cttttctcag taacggttca ccgccggtaa cgacaaccat caggtttttt gcagggtttt
     7741 taatggtgtc aagagctttc agaaaatctt ccatcggcat atcccgatag ttactttgct
     7801 ttgaacaatc gcttccgcaa tgcaaacaat ccaggttgca ccgggtcgtg cactcccaga
     7861 aaaggtagct aagctcgtgg cgccgcgttt caaccgcttt gaacttccgg aacaaggcgc
     7921 gtttaacaga cggaatcatt tcccgttttc cagtttaata tcgaggtaga ttttatcttt
     7981 cacgatagag acgaggctgt ctttcctgat aaatgttccg ttgtcgactc catccacatc
     8041 ttcgaaactg atttgaagct gatctgacgg tgtaaaaaga aaacatcccc ttgtcgtcgg
     8101 tcaccgtatg ttgcaatgag gccgccgaaa ctttgatgcc tttaatgggt tcgccggttg
     8161 ttttggaaac tactttgcct tctacaaaca catccaagcc aaaatcctgc ggcgtaccgt
     8221 aacaagcctg aaatataaaa agcgctgttg taaaactcaa tccgcccaca atgtttctta
     8281 tccaattttg cctcataatg ctatttttta ggttatcaaa tttgagactg acctctttta
     8341 ttcagatgca gttttaagaa aaacgctgcg ccagacaagg ttaaatttta ttaattgctc
     8401 aacgtccaag caaatcggga cgcaaccgtt ttgtacgttc atgcgcctgt tccagccgcc
     8461 attctgcaat tttacgttcg tgccccgaaa gaagcacatc gggtacacgc cagcccttgt
     8521 attccgcggg acgggtataa acggggggtg ccagcaaatt gtcctgaaac gaatcggaga
     8581 gtgccgactg ctcatcacca atagccccgg ggattacccg gacaacggca tcggcaatca
     8641 tggctgcggc gagttcccct ccggtaagca caaaatcgcc gatggatatt tcacgggtta
     8701 ccaggtgttc ccgtacccgg taatccacac ctttgtagtg cccgcaaagt atgataaggt
     8761 tctccagaag cgacaattcg ttggcggttt tttgcgaaaa ctgttcgcca tcgggcgtag
     8821 tgaaaatcac ctcatcgtaa tcgcgctgcg atttcaggtg actgatacag cggtctatcg
     8881 gttcgatttg caacaccatc cccgcttcgc ctccaaaagg gtaatcgtcc acacgacggt
     8941 gcttgtcgag cgtgtaatca cgcaggttgt gcaggccgaa ctgcaccagt cctttatctt
     9001 gcgcccgctt tagaatggaa caattaagcg ctccttctat catttccggc aaaacagtaa
     9061 ttatatcgat tcgcataggg gtatggttaa gagcctgtac cggttccttc ctgtgaacta
     9121 ccgccctgca aaacgtcctc gttggtaatg gtgcgctgaa ctttccttac cgaagatttc
     9181 aagtctccga aacggtacgt caggctcagg cggaaagtgc gcatcgggtt ttgaaaattc
     9241 attttctgaa cgaatccttc acccgttgtt gtcagcttga aatcgcggta cttgtgaaag
     9301 ggagtggttg tattaaggga aacatccagc tttttgttaa agaaactttt catggcgctg
     9361 aacgaataga aataaaaggc cgattgggta gtttgcagtt gtatgttgct cgtaaatatt
     9421 cctccgtttg cactcagcct cacgtctttg ggcaatgtga atgttatccc tccgaaaccg
     9481 cgacccgaaa atccgctgtt ccttaccccg gcattctccg tgctttgaat atcggtataa
     9541 ctgagggagc cgttcacgtt catccggata accggtgtgg gcgtccaact aagatatcca
     9601 tcaagcccta ttgattgatt tctcccgata ttatcgtagg tattgtgagt taccccttct
     9661 cggccttgat attcatctat aaatattctt gatgtgatgg cgttttgggt aaacgaatag
     9721 ctaagcgtgg cgttgaagtt cactttttgt gaaaacgaac cgtaattgat attaagatta
     9781 tgggtctgtt ccgcgtccag gttaggattt ccgtaacgga tgttgttggg atcagaatcg
     9841 tttatatagg gattcaggta ccagataccc gggcgtgata tccgcatatt gtaaccccac
     9901 cgcagggttt tcgtcattcc caactgatag gagaaagcgg cagaaggaac catatcgaag
     9961 aaatcggaat gcacaaccgt atcggtttgc acgctcataa aatgtatctt ctgggcagtg
    10021 ttttctcccc gaagccccag cttcaaaccg aattttccag cttttaatcc gtatccggca
    10081 tatcccgatg caatccgttg ttcgtggtcg aggtcattct tacggctttt atcctcttcc
    10141 cattgccctc cgtcatccgg attcaggaaa gtattgtcac cccggctgga gttatcgcga
    10201 aatatatact tcaatcccac ttctatgctg tgtttgtcgg taagaggatt cacgtaatcg
    10261 acttgtccag tgtgctcttt acctccggca ttgttgatgc tcttcatgcg atagccctta
    10321 gggtaaaaat aattgacaac ctgactgtat tcactttcgt actcactgtc gttggggttc
    10381 ctctcaaaac ggtaggaaac agtaagtaac tcgcctttct ttttaaaagt ccgctgataa
    10441 tcggttgtca gtgtaaatga cccgaaatgg tttatcgaat tattattcaa caggtacgaa
    10501 taatccctcg cgccttgaga aaggacatct tgtgatgaat tggaatgaaa tttcccccca
    10561 aattgtccga cagaaacgtt aaacagattc agtgtatcag gctcgtagct aagctgcgta
    10621 ttgtaaaaca gtccacctcc gtagtttttg gaagttccgt cctgtgtcaa ctcatttacc
    10681 ggacttggag ccatttcttc ccgggtaagt gatgtttttg cctccggcct gttgtggtaa
    10741 aaataacttc cgtttccggt aagtccgaac ttgccgtatt ttaacgacag ataggcattg
    10801 ccaccatatc ccccaaacgt atcgccgttg gctccaacag aacccgaata gccttcgtct
    10861 acccgtttat cggtaatgat gtttatgatt ccacccacgc cttcggcatc gtatcgtgct
    10921 ccgggatcgg taatcacttc cacgtccttt atactgtttg ccggcatgct tttcaacact
    10981 tgcgacgggt tgttcgaaat catgtttgac ggtttcccgt tcaggtatat cttaaagctt
    11041 gatgaacctt tcagctggat attatcttca ccgtcgaccg ttaccagggg taccttacga
    11101 agcaaatcca acacgttaga ggtagatgat tcgggatcgt ctttggcgct gtaggtaagt
    11161 ttatcgattt ccacctttac caacggacgc tgtgcggtta cactgatttc agacaactcg
    11221 gtagagcttt ccgacatttc aatttgcccg agattgacct gcaattctcc gtttttcacc
    11281 tccacgctgc ggttgaaatt attcatccct acaaactgaa acgtgaatat atagtctccg
    11341 gcctgcagcg tagtggtgaa atttccgctg tcgtcggtag caaatttttt gatggatttt
    11401 tgtggtaccg ctttactggc aaccgaaaca gtggcgtaag ggagtggttg tttatcattc
    11461 gcacttacca gttgtccttt cacattagtg ccctccgcat aaaccacaac ggaagtcagc
    11521 aggaggatca gtgataatag agtttgtttc ataaaggtac ttattaaaat tttagcaaaa
    11581 gtagttaata aattcaagag gcaacctata aaagcacccc cgttacgata atttagtaaa
    11641 ggcagtattc tttgatgctt ctttttgcat ttttaaacat tggacttgct cttaccctgt
    11701 ttagacgcaa tattgtccgg ttttcttaca ctaatagcgt taatcttgta caatgattac
    11761 ggggggaaaa aaaattctta cttttgcaat taaaaacaaa agtcgtatgc tcaacattat
    11821 cctcatcata ctatcgggca ttgtcgtggg atattttgcc agaaaactcc cgcaagtgaa
    11881 gcatgtggga tccgttataa gcctgatcat tatgttgctg ttgtttttcc tgggcgtttc
    11941 ggtaggtgca aatgaacaag tagtgacaaa tttcagtacc atcggccttg acgcattgat
    12001 cattagtttc ggggcgactg tgggaactgt tctctgtgcc tggctggtgt actcgacgtt
    12061 tttcagaaga aagggtaaaa attcatgaag caatccctta tctatcttct gctttttgcc
    12121 ctgggtgtca ttcttgctct ttggggacac atccccgacg tgttgctgaa agacgatatc
    12181 tcgaaatgga ttctttacgg actgttattt tttgttgggg tacagatagg ttccggcaaa
    12241 aacatgttta cggccgtgaa gcgattcggg ttcaagatcg ccctagtacc cgttgccacc
    12301 accgttggaa cgtttgcagg agctgcacta acgagccttt tgctcccgga aaggagcttg
    12361 atcgattgcc tgagtgtagg ggctggattt gcatactatt cgctatcgag catcctcatc
    12421 tccgagtttc gtggagctga actgggtacg gtagccttgt tggctaacat catgcgtgag
    12481 tttattgtcc tggtttttac gccgtggctg gtaaaatatt tcggaaaact atcccctatt
    12541 tgcgctggtg gcgcgaccac catggacaca accttaccca tgatcaccaa atattcggga
    12601 tccgactatg tggttgtggc acttttccac ggaatggtca tcgatttttc cgtacctttg
    12661 tgggtcagtt ttttccttac cttgtaaaag ataatgaaat cattttttct aaccatactc
    12721 tttttctatg taacggccaa cgtatatgca gctatcagga tataccagct tattccggca
    12781 aatgcgtggg tcagaattgt cgtaatgggt atttttctgc ttggatttgc cagcctgctt
    12841 atttttttcc ggtttggcga ggctatgcct gtcggaactg ccggcttctt ctatcggttc
    12901 gggacatcat ggatgatcgc cttcctgtat gttttcatgc tcattctctt tgtcgattta
    12961 ttcaggatca tcaactgggt tgcacctatt gccgacaaag aaactgttcg tggcattttt
    13021 catcacaacg cgctaactgc cgttacaggt ttggggctta cggcgttgat acttttgtac
    13081 ggtaactggc aatatcacaa caaaaaacgt tctcacataa ccatcaaaac cgaaaaaatc
    13141 gataaaccga tgagaattgt cggtatcagc gatttacacc tgggatacac catttcaaag
    13201 aaagagctga gcagatgggt ggagatgatc aatgctgaaa atccggacat ggttattatt
    13261 ggcggagact tggttgacaa ccagttgcga cccgtatgga tgcattcgct cgacagggaa
    13321 ttgctgaaaa ttaaagcacc cttgggagtc tatgcctgta ccggtaatca cgaatacatc
    13381 tcgggtatta aaaacagtgc agatttttac acccggtcgg gaatcacgct attgcgtgac
    13441 tccgtgcttc aaacgaatgg gttaacaatc atcggacgag aagatcattc ccggaagaac
    13501 cgaaagacat tgccggaact gataaaaaac agtgacaacc ggactttttc tatcctgctc
    13561 aatcatcagc cttacgatct ggatgaggca gtgcgggaag gaatcgattt tcagttctcc
    13621 ggacatacac atcggggaca ggtctttcca gcttcgttga taacagataa aattttcgaa
    13681 ctctctcaag ggtatattca aaagaagaat acccatttct acgtttcttc aggcttgggc
    13741 atttggggcg gaaagttccg cattggcacc cgctcggagt acctggtact ggatttggag
    13801 aattaaattg tttacttttt tatgcgttca cttatcgtcg cactcattat acatttcacg
    13861 ctcaacatcc ttgttttcct gaagggttgg gatgttttta aaacgaaaaa actcctccgg
    13921 actttttgga tggttatttt cgccttcgaa cttttggtgt atcttacggg atttgcattc
    13981 taccgccatc tccctcctga aattatccat cccatccgca tgatggggac cagctggatg
    14041 cttttcctgc tatacctggg gggcctggta ttgataggtg actttctgta cctggcgtcc
    14101 cggaagaaac tcacccgtcc caaagaatta ttgaaccagc cagcaaaaat gaaactcacc
    14161 ctctttctct cctcctttgc agtggtaata ctcacgttgt cttatggcaa ttacaaattt
    14221 aaccatccag aggtacgaca ggtggatata caggttggta aatcagctgg aaaaatggac
    14281 tccgtgagga ttgcgatggt tggcgactta catttgggat acctgatcaa ccgggatgac
    14341 gcacaaagaa tggtggattt aatcatggag caggaacccg atttgatcct ttttgtgggg
    14401 gatatcctcg actctagcat cgaacccgtt cagggacaaa ggatggacga ggaactccgg
    14461 cggctgcagg cgcctctggg cgtatattcc tgcacgggca accacgaata ccgttacgaa
    14521 gccg
//
LOCUS       DAOSDR010000021        14346 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrimonas sp. isolate AMR_MDS_1826
            NODE_1670_length_14346_cov_5.469596, whole genome shotgun sequence.
ACCESSION   DAOSDR010000021 DAOSDR010000000
VERSION     DAOSDR010000021.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863355
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrimonas sp. (wastewater metagenome)
  ORGANISM  Petrimonas sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Dysgonomonadaceae; Petrimonas.
REFERENCE   1  (bases 1 to 14346)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14346)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:10:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,720
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 35
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14346
                     /organism="Petrimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1670_length_14346_cov_5.469596"
                     /isolate="AMR_MDS_1826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2023866"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            225..656
                     /locus_tag="PLI30_01625"
     CDS             225..656
                     /locus_tag="PLI30_01625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI78283.1"
                     /translation="MMKQIFSILLSFILLASHMNLTMGTHFCGGEAVETKVLLGETHL
                     GCEMMDLVEPCDGSEHSNQNQVRFNNVPCCQNEFQTIQGTADFVKEAAQTVFNVDFAL
                     AYIYTTLILDLVPKSSHQVYTECISPPLEKDIQVLFQTFLI"
     gene            753..4577
                     /locus_tag="PLI30_01630"
     CDS             753..4577
                     /locus_tag="PLI30_01630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006844390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HOI78284.1"
                     /translation="MLNKIIKYFLENKLVTVLVLTGFVAWGIVTAPFGWQVGGLPSDP
                     VPVDAIPDIGENQQIVFTPWNGRSPQDIEDQISYPLTTYLLGIPGVKTIRSSSIFGFS
                     SIYIIFDEDIEFYWSRSRILEKLSSLPSGLLPDGVQPTLGPDATALGQVYWYTLEGRD
                     KDGNPTGGWDLHEIRTVQDFYVKYGLNATEGVSEVASIGGYVQEYQIDVNPDALKAYN
                     IPLHKVMQAVQKANRDVGAKTIEINQAEYLVRGLGYVKKTEDIEKAVVAVQENVPIRI
                     KDIGVVTLGPATRRGALDKDGAEVVGGVVVARYGANPLQVINNVKEKITEISPGLPKK
                     ILANGVESQLTIVPFYDRSELIYETLGTLEEALSLQILITILVIIVMIYNLRASFLIS
                     SLLPIAVLMVFIAMRYFGVDANIVALSGIAIAIGTMVDLGIILSENIVKHVDEAPPGQ
                     KLITTIYNGSAEVSSAILTAVSTTIVSFIPVFTMQAAEGKLFGPLAFTKTFALVAALI
                     VSLIILPTLAHWFFGFKIKNPRHNKWVNMLLIAVGFIGLFSNVAWAGILLILFGGIPL
                     IKPLIRDKKLFRKPLFGKTLDNIELILVVVGVVWLLAKYWLPLGAGKSVLLNFVFVAL
                     LVGFILGAFTLLQFSYRRILRWCLNNKVKFLMIPVFLMLLGVNIWMGFSNIFGFLANG
                     ADKMGWNIRTTAVWSGLNHSFPGMGKEFMPSLDEGSFLLMPTSMPHSGVEYNRKVVGQ
                     LDMMIANIPEVELSVGKLGRVESALDPAPVSMYENVINYKSEYELNDKGHRKRFKTDG
                     DGRFVLKNGEAYSNDELLTKNISPDELVPDDKGNYFRNWRKHIKSSDDIWKEIVAVTK
                     IPGVTSAPKLQPIETRLVMLQTGMRAPMGIKVYGSDLQTIEEFGLKLEGILKQVPSVK
                     AEAAFADRIVGKPYIHLNINRDEISRYGLNVEDVQQSIETAIGGMKVSSTVEGRERFP
                     IRVRYPRELRDDPESLGKILIPTPTGAQIPLSQLVDMEYVRGPQAIKSENTFLVGYVL
                     FDKKEGFAEVDVVNDAQEMIQQKIDAGELIVPNGVNYKFSGSYENQVRAEKRLSIIVP
                     LVFLIIFLILYFQFKSVTTSLMIFTGVAMAFSGGFIMLWLYGQGWFADFTVFGTNIRE
                     LFQMNTVNLSVAVWVGFIALFGLATDDGVLMGTYLDQSFKRNRTKTIEEIRAAVVEAG
                     ERRIRPAVMTTTTTIIALLPVLTSTGRGADIMVPMAIPAFGGMIVAAITYYIVPTFYC
                     MREEYKLKNSKS"
     gene            4574..5806
                     /locus_tag="PLI30_01635"
     CDS             4574..5806
                     /locus_tag="PLI30_01635"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HOI78285.1"
                     /translation="MIRYIAILIATLLFSSLSFAQTLDDYFKIAAENNPGLQAMHKEY
                     EAALQKVPQVSTLPDPTFSLGYFISPVETRVGPQQAKFSLTQMFPWFGTLKAQGNVAA
                     LMAEAKFQNFIDARNRLYFQVASAYYPLYELKDFVRIEQENISILESYKTIATQKFKN
                     GTGTMVDVLRVDIMLKDAQTNLSILRDKEKPLLTTLNKLLNRSENEPVQISDTLKSVI
                     LLDNYRKDSLIVANPKLKALDLKLQASQAAEIVAQKQGLPKLGIGLDYVLVGERTDIS
                     MPDNGKNVLMPMVSISIPIFRAKYNASVKESQLMQESYTLQKQEVTNTLFSEYEMVLF
                     QVQQQLQLISLYQQQIQTSQQSLNLLFTSYGNSGKEFEEALRMQQQLLKYQKMSATAL
                     VQYHIAVEKINYITSKSY"
     gene            5808..7616
                     /locus_tag="PLI30_01640"
     CDS             5808..7616
                     /locus_tag="PLI30_01640"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HOI78286.1"
                     /translation="MKAIDKKAIIIAAATLVVGLLAGWLLFGGNKSNSHDDHEHVKTE
                     INGETVWTCSMHPQIRQNEPGDCPICGMDLIPLEDDQNADIDPNAISMSPTAMQLANV
                     STAFVGKMNPVKQVRLNGKVQADERLVYSQSSHIPGRIEKLLVNFTGEFVNKGQVIAY
                     IYSPELVTAQEELLEAQKIAETQPQLFVAAKEKLKNWKLSEKQIEEILTSGMTKDEFP
                     VVADVSGYTISKKINLGDYVRKGETIYEIADLSKVWVLFEVYESDISWIKKGDKVEFT
                     IASMPGESFISIITFLDPVIDPKTRVAKARVEYNNSNGKLKPEMFASGMVQAKLANKS
                     NSIVVPKTAVMWTGKRSVVYVKTNSEQGVNFIMREITLGSALGDSYVVEEGLMEGEEI
                     AVNGTFSIDAAAQLAGKPSMMSPEGGQVMTGHNHGGTTTESNVSHESHEEQNKSTTSK
                     TLSIDKKAKEALQPIYNTYLNWKDALTNDDFKEAQKNAANFKTALKKVDMGLFKDEAH
                     MVWMDFQGRLAKNLEHIQHFSDIEQLRKAFQSVSVTMIEMTNSFAPLGKTIYVQHCPM
                     ADNNKGADWLSSEKEIKNPYFGSSMLKCGEVTKEIK"
     gene            7649..8023
                     /locus_tag="PLI30_01645"
     CDS             7649..8023
                     /locus_tag="PLI30_01645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HOI78287.1"
                     /translation="MKTKVLTLVCMFLLGTSMVFAQAKTEKFEVNGNCGMCKTRIEKA
                     ANSVDGVTKADWVQETKMLTVAFDSTKTTVHKVQMAIAKVGHDTPMHKASDEVYNALP
                     GCCKYDRTVAKKESTGHEGHKH"
     gene            8257..8862
                     /locus_tag="PLI30_01650"
     CDS             8257..8862
                     /locus_tag="PLI30_01650"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HOI78288.1"
                     /translation="MPNGWVWGRLSEIVVITGGGTPSMNNPDFWNGNIPWVTPKDMSS
                     NQINGSEMKITQLGLDNSSAKLIDDGSILIVGRSGILRRRLPVAINLIPCSVNQDMKV
                     LTPIFKIMNRFLQMMLYGLEQNILRNYVKFGMTVHSLRYDEFSLMPIPIPPLAEQHRI
                     VNKIDQLIQFCEEIETTIKQNQNYAQELLQVALKEALTPVK"
     gene            8964..9401
                     /locus_tag="PLI30_01655"
     CDS             8964..9401
                     /locus_tag="PLI30_01655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI78289.1"
                     /translation="MRVENVFKKCFIHIIDDLKAQGNNLTSISKSMGYTSTSQLHNAM
                     SEDGLPSTKAIIALIENYKVNPSYLFLNQGDIYSDESELERVKTELSKYTQMHNEAVK
                     TVNSLNEMVKTLEQRNADLIDITADALKFHKQSKGDIEPRNEK"
     gene            complement(9535..9605)
                     /locus_tag="PLI30_01660"
     tRNA            complement(9535..9605)
                     /locus_tag="PLI30_01660"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9571..9573),aa:Cys,seq:gca)
     gene            complement(9678..9890)
                     /locus_tag="PLI30_01665"
     CDS             complement(9678..9890)
                     /locus_tag="PLI30_01665"
                     /inference="COORDINATES: protein motif:HMM:NF025733.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich CWC family protein"
                     /protein_id="HOI78290.1"
                     /translation="MSDNKPTLKICPRCGRSFECLHAPGCWCFDYSISPENLKKLKLE
                     YDNCLCPVCLPDFGEKAEKGSVTPTD"
     gene            complement(9883..10248)
                     /gene="folB"
                     /locus_tag="PLI30_01670"
     CDS             complement(9883..10248)
                     /gene="folB"
                     /locus_tag="PLI30_01670"
                     /EC_number="4.1.2.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018339357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HOI78291.1"
                     /translation="MGMISIEGMEFFSYHGHFKEEQVIGTRFMVDLFIQVDTKQAERS
                     DSLHDTVNYLSVYETVKQEMEKKSHLLEHVAHRILKAVHTRFPDVTDAEVKISKLNPP
                     LGGKMDRVCVTLSSDDIYE"
     gene            complement(10313..11014)
                     /locus_tag="PLI30_01675"
     CDS             complement(10313..11014)
                     /locus_tag="PLI30_01675"
                     /inference="COORDINATES: protein motif:HMM:TIGR00697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:1990397 - queuosine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="queuosine precursor transporter"
                     /protein_id="HOI78292.1"
                     /translation="MTDAVQSGKVHFRYFDVIMALFVAILLISNLASTKITSLWLFTF
                     DGGTILFPLSYIFGDILTEVYGYQRSRKVIWTGFGAALLMSLVLWIVQELPPASDWPN
                     QQAYESLLGFVPRIVLASLVAYFAGAFSNAYLMSKLKIRTKGKYLWVRTIGSTLLGEG
                     IDTAIFCLIAFYGTMPDSLLISIIISNYIFKSGVEIVFTPLTYRIVGFLKKREQVDVF
                     DTGISYNPFRYFVKE"
     gene            complement(11123..12466)
                     /gene="mgtE"
                     /locus_tag="PLI30_01680"
     CDS             complement(11123..12466)
                     /gene="mgtE"
                     /locus_tag="PLI30_01680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019538641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HOI78293.1"
                     /translation="MGETEITFFEDLIRDKDWSTLRHELEDMDPVLIAEIIDELPEQD
                     DVILFRLLPREQAKNTFQYLEHDTQEQIIEGLAKNVNRVAGLLNDLDPDDRTAFFEEL
                     PGNITQRLIQLLSPEERIIATRLLGYPEHSIGRLMTPEFVAVRPEYTVEQALKHIRMY
                     GRDSETLNLIYVVDENWKLIDDIRIREIILADPGQHIRDLMDEHFVSLNAFDDQEVAI
                     RVFQDHDRIALPVLDSDGLLLGIVTVDDVMDVVEEETTEDFHKFGSFQAAIVNPLKAK
                     IFFLYQKRILWLIVLVFMNVFSGAALATFEGVIQSAVSLIFFLPLLIGSGGNAGAQSA
                     TLMIRSLAIGDVETKDWFRLLGREFLVSFLLGITMAAGVSLIASVRAPQYIAVVAITM
                     VLAVMTGSLIGIITPFIFTRLKLDPATASAPLITSIADISGVVIYFSIASWYMGL"
     gene            12790..13440
                     /gene="def"
                     /locus_tag="PLI30_01685"
     CDS             12790..13440
                     /gene="def"
                     /locus_tag="PLI30_01685"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: protein motif:HMM:NF013492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HOI78294.1"
                     /translation="MNRLNGILLVHAVLLSVCLQAQQHSDPERALVLSGDTTAMMRVL
                     TTSHAGDAAILNAECRDLDPFDPMVRLLSQRMFLSMRDSANPGVGIAAPQVGIKRNAF
                     WIQRFDKQGFPFEFMINPVILSYSALKRRGGEGCLSIPGVRGEVLRSYAIMVEYRDFS
                     GGWHTELLEDFTAVIFQHEYDHLKGILFTDRMDQQLSFPVYPLPEGTELFLGPPGM"
     gene            complement(13443..13781)
                     /locus_tag="PLI30_01690"
     CDS             complement(13443..13781)
                     /locus_tag="PLI30_01690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI78295.1"
                     /translation="MKPVKSLLPASKWLLRITLLTWLLLQHGQTILALNYQSQGFYLA
                     LAFVLFGVLLFAGGFTSKPSLTVVSGLLLSLLFAYTIYLGFVPRVTAVQVTHLLLLSV
                     CLYFIASGNK"
     gene            complement(13778..>14346)
                     /locus_tag="PLI30_01695"
     CDS             complement(13778..>14346)
                     /locus_tag="PLI30_01695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009317046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HOI78296.1"
                     /translation="KALSELRLEMGNLLGLRNPEVFAPLWVVDFPLLEWDDETQRYYA
                     MHHPFTSPKPEDIPLLDSDPGKVRANAYDLVINGVEIGGGSIRIHNKDLQKKMFTILG
                     FSDEDAQAQFGFLMNAFEYGAPPHGGIAFGFDRMCSLFGGSDSIRDFIAFPKNNAGRD
                     VMIDAPAPIKQEQLDELKLVINIQDQRE"
BASE COUNT         4323 a         2894 c         3220 g         3909 t
ORIGIN      
        1 ccacattgca cacattgcca aagccccaca agccaacgct gcaaccaaag ctttgccaaa
       61 aagtgcaatt ttgcctacgc accataacag taaattacga attaagcagt tactactgtt
      121 tgattttcaa ataaataatt ctcagaattc atgtcaggat taaaaaaatg tacgacaaat
      181 tgaaagtgaa tgaaaacaat tttagtaatt ttgcgtttaa ctaaatgatg aaacagattt
      241 tttccatatt attatctttt attctcttgg caagccatat gaacttgacc atgggtacac
      301 acttttgcgg tggcgaagct gtagagacta aggtattatt aggagaaaca catttgggct
      361 gtgaaatgat ggatttggta gaaccctgcg atggttctga acattctaat cagaatcagg
      421 ttcgttttaa taatgttcct tgctgccaaa atgaatttca aactattcaa ggtacagctg
      481 attttgtaaa agaagccgct caaactgttt ttaatgttga ttttgcttta gcctatattt
      541 ataccacctt gattttggat ttagttccaa aatcatcaca tcaggtatat actgaatgca
      601 tttcccctcc tcttgaaaaa gacattcaag tactttttca aactttcctg atttgatttt
      661 tgcgtgaact tatgattcca tattacaatt tgtagtgtgg ttgtcgtgta ttgtattaaa
      721 agctaatttt ttcatgtgaa aatcattgta tcatgctaaa taaaataatc aaatatttct
      781 tagagaacaa gttggtaaca gtacttgttc taaccggatt tgtcgcatgg ggcattgtta
      841 ccgctccctt tggctggcaa gtgggtggat tgccgtcaga ccctgttccg gtggatgcca
      901 tacccgatat tggcgaaaat caacagatag tttttactcc ctggaacgga cggtcgccac
      961 aagatattga agaccagata tcttatccgt taactactta tcttttagga attcccggag
     1021 ttaaaaccat tcgtagttct tcaatttttg gattttccag tatctatatc atttttgatg
     1081 aagatataga attttactgg tcacgctcac gaatattgga aaagttaagt tcactcccct
     1141 ccggactatt gcccgatggt gtgcaaccca cattgggtcc agatgcaact gctttggggc
     1201 aggtttactg gtacacgctc gaagggcgtg ataaggatgg aaacccgact gggggctggg
     1261 atttacacga aatacgcacc gttcaggatt tttatgtaaa atatggacta aatgcaaccg
     1321 aaggtgtatc ggaagtagca tccattggtg ggtatgtgca agaataccaa atagatgtaa
     1381 atcccgatgc attaaaagct tacaatattc ctttacacaa ggtgatgcag gcggtacaga
     1441 aagcgaatcg tgatgtgggg gctaaaacca tcgagataaa tcaggctgaa taccttgtcc
     1501 gtggtttagg ttatgtgaaa aagaccgaag atattgagaa agccgtagtg gctgttcagg
     1561 aaaacgttcc cattcgtatt aaagatattg gtgtagtaac acttggtccg gcaacacgaa
     1621 ggggagcact cgacaaggac ggtgcagaag tagtaggtgg tgtggtggta gcacggtatg
     1681 gagctaatcc attgcaggtg attaacaatg taaaagaaaa aattacggag atttcacccg
     1741 gtttgccgaa aaagattttg gctaatggag tcgaaagcca attgaccatt gttccatttt
     1801 acgaccgctc ggaattgatt tatgagacac ttggcacatt ggaagaagcc ctttcacttc
     1861 aaattctcat aacaatactg gtcatcatcg tgatgattta taacctgagg gcatcttttt
     1921 taatatcaag cttattgccc attgcagtac tcatggtttt tatcgccatg cggtattttg
     1981 gagttgatgc caatattgta gcactatcgg gaatcgccat tgccattggt accatggtcg
     2041 atttgggaat aattctctcg gagaatattg tcaaacatgt agatgaagcc ccacccggac
     2101 aaaagctgat aacaacaata tacaacggct cggcagaggt tagctctgct atccttactg
     2161 ccgtttctac cactatcgta agttttattc ctgtttttac catgcaggct gccgagggta
     2221 aattattcgg cccgttggca tttaccaaaa cctttgcttt ggtagcggca cttatcgttt
     2281 cgcttatcat tttaccgacg ctggctcact ggttttttgg ttttaaaatt aaaaatccac
     2341 gccataacaa gtgggtgaat atgctgctta ttgcagttgg ttttattggt ctattcagca
     2401 atgttgcatg ggctggtatt ttgttgattt tatttggagg tattcccctt attaagccat
     2461 taattagaga caaaaaactg tttagaaaac cacttttcgg aaaaacctta gataatattg
     2521 aattgatttt agtagtagtt ggtgttgtct ggcttttggc aaagtattgg ttgcctttgg
     2581 gagctgggaa aagtgtgctt cttaactttg tttttgttgc tttgctggtt ggctttattt
     2641 taggggcatt taccttattg cagttttcat acagaaggat tttgcgatgg tgtttgaata
     2701 ataaagttaa atttcttatg attcctgttt ttttgatgtt gctgggtgta aatatctgga
     2761 tgggtttctc aaacattttt ggatttctgg caaatggagc tgacaaaatg ggttggaaca
     2821 ttcgtactac tgctgtttgg tcaggactta accattcatt tcccgggatg ggtaaagaat
     2881 ttatgccctc acttgatgaa ggcagttttt tgcttatgcc tacctcaatg ccgcactcag
     2941 gagttgaata caacagaaaa gttgttgggc agttagatat gatgattgca aatattcccg
     3001 aagtggaact tagcgtaggt aaattaggcc gggtagaatc tgcacttgac ccagcacctg
     3061 tttcgatgta cgaaaatgtt atcaattata agtcggaata tgaactcaat gataaagggc
     3121 atcgtaaacg ctttaaaact gacggcgatg gtcgctttgt gttgaagaat ggggaagcat
     3181 acagcaatga tgaattgctg actaaaaaca tttcgccaga cgaacttgtg ccggatgata
     3241 agggaaacta tttcagaaac tggcgcaaac atataaaatc atccgacgat atttggaaag
     3301 aaattgttgc ggttactaaa ataccgggcg ttacttctgc cccgaaactg caacccatcg
     3361 aaacccgatt ggtcatgttg caaacaggaa tgagggctcc aatgggaatt aaagtttatg
     3421 gttccgacct tcaaaccatc gaggagtttg gtttgaaact ggaaggtatt ctaaaacaag
     3481 taccctcggt gaaagccgaa gcagcctttg ccgaccgtat tgtagggaaa ccttatattc
     3541 acctaaatat caaccgtgat gaaatttcac gttatggatt gaacgtggaa gatgtgcaac
     3601 aaagcattga aacagcaatc gggggcatga aagtatcgtc aacggttgaa ggacgtgagc
     3661 gttttcccat acgagtccgt tatccgagag agctaaggga cgacccggaa tcgcttggaa
     3721 aaattcttat tccaacaccc acgggggctc aaattccact tagtcagtta gttgatatgg
     3781 agtatgtgcg tgggccacaa gccattaaaa gtgagaatac ctttcttgta ggttatgtgc
     3841 tgtttgataa aaaggaaggt tttgctgaag tagatgttgt aaacgatgca caggaaatga
     3901 ttcagcaaaa aattgatgca ggagaattaa ttgttccaaa tggtgtgaat tataaattct
     3961 ccggaagtta tgaaaatcag gtgcgtgccg aaaaacgttt gagcattatt gttccgttgg
     4021 tctttttaat catcttcctt atactctatt ttcaattcaa atctgtaaca acttcactca
     4081 tgatttttac cggagtggct atggcattca gtggcggttt tattatgctc tggctttacg
     4141 ggcaaggctg gtttgccgat ttcaccgttt ttgggacaaa catccgtgag ctttttcaga
     4201 tgaataccgt gaacctaagc gttgccgttt gggtgggctt tattgccctg ttcggattgg
     4261 caaccgatga cggggtattg atgggaacct atctcgacca aagttttaaa cgtaaccgaa
     4321 ctaaaacaat agaagaaata cgggcagcgg ttgtggaagc aggtgagcga agaatcaggc
     4381 ctgctgtaat gaccaccaca actaccatca ttgctttact tccggtactt acttcaaccg
     4441 gtaggggcgc ggatataatg gtgccgatgg ccattccggc ttttggcggt atgattgtgg
     4501 ctgccatcac ctactacatc gttcccacgt tttactgtat gcgtgaagag tataaactta
     4561 aaaattcgaa atcatgataa gatatatagc aatacttata gcaacgcttt tgtttagtag
     4621 cttgagcttt gctcaaacac tggacgatta ctttaaaata gctgccgaaa acaatccggg
     4681 gttacaggct atgcataaag aatacgaagc tgctttacag aaagtgccgc aggtaagtac
     4741 cttgcccgac cctacttttt ctttagggta ttttatctct cctgttgaaa caagggttgg
     4801 tccgcaacag gcaaagttct cgctaacgca aatgttcccg tggtttggta ctttaaaagc
     4861 gcagggcaat gtggctgctt taatggcaga agcaaagttt caaaacttta ttgatgccag
     4921 aaatagactt tattttcagg tggcatccgc ttactatcct ttatatgaat taaaagattt
     4981 tgttcgtatt gaacaggaaa acattagcat attggaatct tataaaacca ttgctactca
     5041 gaaatttaaa aatggaacag gaacaatggt agatgtgctg cgtgtggata ttatgctgaa
     5101 agatgcacaa accaatctta gtattctgag agataaagag aaaccattgc tgactacgct
     5161 caacaagctt ttaaatcgtt ctgaaaatga accggtgcag attagcgata cgttgaaatc
     5221 ggtgattctc ttagataatt acagaaaaga ttcactgatt gtagccaatc caaaattaaa
     5281 agcgctcgat ttaaaattac aggcaagcca agccgcagag attgttgcac aaaagcaagg
     5341 attaccgaag ttgggtatag gcttggatta tgtattggtg ggcgaacgta ccgatatttc
     5401 tatgcctgat aatggaaaaa atgttctgat gccaatggta agcattagta ttccaatctt
     5461 tcgggcaaaa tacaacgcat cggtgaaaga atctcaactg atgcaggaaa gttacacact
     5521 tcaaaagcag gaagtaacaa atacgctctt ttctgaatat gaaatggttt tgtttcaggt
     5581 tcaacaacag ttgcagttaa tatcacttta ccaacaacag atacaaacct cgcaacaatc
     5641 attaaacctg ctatttactt cctatggaaa ttcagggaaa gagtttgaag aagcactacg
     5701 tatgcagcaa caattgctga aatatcagaa gatgagcgca accgctttgg ttcaatacca
     5761 tattgcagtt gagaaaatca attacataac atcaaaatcg tattaaaatg aaagcaatag
     5821 ataaaaaagc aataataatt gccgcagcaa cacttgttgt tggtttgctg gcaggatggt
     5881 tactgtttgg agggaataaa agtaattctc acgatgacca tgaacatgtg aaaacggaaa
     5941 taaacggaga aacagtttgg acttgctcca tgcacccgca aattcgtcaa aacgagccgg
     6001 gcgactgccc catttgcggc atggatttaa taccgttaga ggatgaccaa aatgccgata
     6061 ttgaccccaa tgcaataagc atgtcgccta ctgctatgca actcgccaat gtgagtacgg
     6121 catttgtggg aaaaatgaat ccggttaaac aagtccgcct taacgggaaa gtacaagccg
     6181 atgaacgctt ggtgtattca caatcatcgc atattccggg caggatagaa aaattgttgg
     6241 tgaattttac tggcgaattt gtgaataaag gacaagtcat tgcatacatc tattcacccg
     6301 agttggtaac agcacaagaa gagctgttgg aggcacagaa aatagccgaa acgcaacctc
     6361 aattgtttgt agctgcaaag gaaaagttga aaaactggaa actctcggag aaacaaattg
     6421 aagaaatcct tacatctgga atgacaaagg atgaatttcc tgtagtagcc gatgtttcag
     6481 gatacacaat ctcaaaaaaa ataaatttgg gtgactatgt tcgcaaaggg gaaacgatat
     6541 atgaaatcgc tgacctatcg aaagtttggg tgttatttga agtgtatgaa tcggatataa
     6601 gctggattaa aaaaggtgat aaagttgaat ttacaattgc ctccatgccc ggtgaatcat
     6661 ttataagcat tattacattt ctcgaccctg taattgaccc aaagacaaga gtggcaaaag
     6721 caagagtgga atataacaac tcaaacggaa agctaaaacc tgaaatgttt gcttcgggga
     6781 tggttcaggc taaattagct aacaaatcaa attctattgt ggtaccaaaa actgccgtaa
     6841 tgtggacagg caaacgctcg gtggtgtatg tgaaaacaaa ttccgagcaa ggagtaaact
     6901 tcattatgcg tgaaataacc ttgggttctg cattaggaga tagctatgtg gttgaagaag
     6961 gtttaatgga gggagaagaa atagcggtaa acggcacgtt cagtatagat gccgctgccc
     7021 agttggccgg aaaacccagc atgatgagcc ctgaaggtgg acaggtaatg acaggtcata
     7081 atcacggagg gacaactact gaaagtaatg tttcacacga aagtcatgag gaacaaaata
     7141 aatcaacaac ttcaaaaacc ttgtccatcg acaaaaaagc caaagaagct ttgcagccaa
     7201 tttataatac ctacttaaat tggaaagatg ccctgaccaa cgatgatttt aaagaagctc
     7261 aaaaaaacgc tgcaaacttt aaaacggcat tgaaaaaagt ggacatggga ctttttaaag
     7321 atgaggcgca catggtttgg atggattttc agggaagatt agccaaaaat ttggaacaca
     7381 tccagcactt ttccgacatc gaacaacttc gaaaagcctt tcaatcggtt tctgttacga
     7441 tgattgaaat gaccaattca ttcgcaccac ttggcaaaac aatctacgta caacattgcc
     7501 ccatggccga taacaacaaa ggggctgatt ggctgagctc tgagaaagaa attaaaaacc
     7561 cgtactttgg tagttcgatg ctaaaatgcg gagaagttac caaagaaatt aaataatcat
     7621 ttttattcac aatttaaatc acaataatat gaaaacaaaa gttttaactt tagtatgtat
     7681 gttcctttta ggaacaagta tggtatttgc acaggcaaaa accgaaaaat ttgaagtaaa
     7741 cggaaattgt ggcatgtgca agacccgcat tgagaaagcg gcaaattcag ttgacggtgt
     7801 taccaaagcc gactgggtgc aagaaacaaa aatgcttaca gtagcatttg attcaacaaa
     7861 aactactgtg cataaagttc aaatggctat tgctaaagtt ggtcatgata cacccatgca
     7921 taaagcctct gacgaagtgt ataacgcact tcccggatgt tgcaaatatg accgtaccgt
     7981 agcaaagaaa gaaagtacag gtcatgaagg acataagcac taaaagaatt ggggttaatg
     8041 gctgccttta attagtatct attaattgcc aacccacagt cgtaagcaca tttgcaagcc
     8101 cacacgagcc aacgcacagt ccaaagcttt gtcaaagagc ctgcaagctg acccacgaag
     8161 aaaggggaac tttaaaatgc cccaccgcac gagaaaggga aaatcaaaaa ggaaaaatca
     8221 ttaccagcaa ttaaacctat agagatacct tttaatattc ctaatggatg ggtatggggt
     8281 aggttgtcag aaattgttgt gattactggc ggtgggacac cttcaatgaa taaccctgat
     8341 ttttggaatg gtaatattcc ttgggtaact cccaaagaca tgagttcaaa tcaaattaac
     8401 ggttctgaga tgaagattac acaattaggg ttagataatt catctgcaaa gttaattgac
     8461 gatggttcaa tacttattgt tggaaggtca ggaattttga gaagaagatt gccagttgca
     8521 attaatttaa taccttgttc tgttaatcaa gatatgaagg tacttacccc tattttcaaa
     8581 ataatgaatc gttttttgca aatgatgcta tatggtttag aacaaaatat cttgaggaat
     8641 tatgtaaaat ttggaatgac cgttcatagt ttacgatatg atgagttttc attaatgcca
     8701 ataccaatac ctcctctggc tgaacaacac cgcatagtca ataaaataga ccaactgatt
     8761 caattctgtg aagaaataga aaccactatt aagcaaaacc agaattacgc tcaagaatta
     8821 ttgcaggttg cgttaaaaga agcactcaca ccagtaaagt aaatgttgac gaataacaca
     8881 ttaggttgat atttgctcat gttaggtaaa ttcataccca taagtttgtt tttctcttat
     8941 atttgttact taaattcaac catatgagag tagaaaatgt ttttaagaaa tgctttattc
     9001 atattattga tgaccttaaa gcacagggta ataacctaac gagtatttcg aagagtatgg
     9061 gatacacgag cacttctcaa cttcataatg caatgagcga agatgggtta ccctccacaa
     9121 aagcaataat tgcattgatt gaaaattata aagtgaatcc ttcttatcta tttcttaatc
     9181 agggtgatat ttactctgac gaatcggaac tagaaagagt aaaaacagaa ctttcaaagt
     9241 atacccaaat gcataatgag gcagtcaaga cggtcaattc tttaaatgaa atggtaaaaa
     9301 ctttagaaca aagaaatgct gatttaattg atatcactgc cgatgcctta aaattccata
     9361 aacagagcaa aggagatatt gaacccagaa acgaaaagta aaattttact ctttacttaa
     9421 ggttttatat cttttaagta aaatttgact tggttagaat ttgccccaat gccccagaaa
     9481 attgccccac aataaaaaaa acctcttgat tatcaagagg tttaagaagg gttgaggtct
     9541 cgagcggatt cgaaccgctg tagaaggttt tgcagacctc tgcctagcca ctcggccacg
     9601 agaccctttg gggatgcaaa tatacaaaaa ttccaaaaaa acgaatataa aagatttcct
     9661 gcaataatta cagtaaatca atcagttgga gtaactgacc ctttttctgc cttttcaccg
     9721 aaatcgggca ggcatacggg gcagaggcag ttatcgtatt caagctttaa ctttttaagg
     9781 ttttcagggc ttatactgta atcgaaacac cagcatcccg gggcatgcag gcactcaaaa
     9841 ctcctgccgc aacgcggaca gattttcagc gttggcttat tatcactcat agatgtcatc
     9901 gctgctcagg gtaacgcata ccctgtccat tttaccgccc agtggaggat tcagtttgga
     9961 aatttttacc tcggcatcgg tcacatccgg aaaacgggta tgaaccgctt tcaggatacg
    10021 gtgggctacg tgctccagca aatgggattt tttctccatt tcctgtttaa ccgtttcata
    10081 aacagaaagg taattcaccg tatcgtgaag gctgtcgctg cgctctgcct gttttgtatc
    10141 cacttgaata aacaaatcga ccataaagcg ggtaccaata acctgctctt ctttaaaatg
    10201 gccatggtag gagaaaaact ccataccctc tatagaaatc atgcccatga tgcgtgcgtt
    10261 tttaacaaag ataggcaaaa actgctcagg gtattttcag caggtaaact tgctattctt
    10321 tgacaaaata acggaacgga ttatagctga tgccggtatc gaatacatcc acctgctcgc
    10381 gttttttcag aaatccgacg atgcggtagg tgaggggggt aaacacgatt tcaactcccg
    10441 atttgaaaat gtaattggaa attattatcg agatcagcag actgtcgggc atggtaccat
    10501 aaaaagcgat caggcagaaa atggcggtgt cgatgccttc acccagcagg gtggatccta
    10561 tggtccgaac ccacaggtat ttcccttttg tcctgatttt cagtttcgac atcaggtaag
    10621 cgttagaaaa ggcgccggca aagtaagcaa caagactggc cagtacaatg cgcggaacaa
    10681 agcccagcag agattcatag gcctgctgat tgggccagtc ggaggccgga ggcagttcct
    10741 gcactatcca aagcaccagc gacatcagca aagcagctcc gaaaccggtc cagattactt
    10801 ttcttgagcg ctgataaccg taaacttccg taaggatatc cccgaaaata tagctgagcg
    10861 ggaagagaat ggttccgccg tcgaacgtaa acagccaaag ggaagtgatt tttgttgagg
    10921 caaggttgga gatcagcaga atggccacaa aaagagccat gattacatca aaatacctga
    10981 aatgaacctt gccggattgt actgcgtctg tcatttgata ttgtattggt caggatatcc
    11041 ggctgcaaaa gtacaacaat ccccctgaaa agagattcct tcccggaaag gcaatcaaac
    11101 ccggcagggg aggagcggga ctttacaaac ccatatacca ggaagcaatg gaaaaataga
    11161 tcacaactcc ggaaatatct gcaatggatg tgatcagcgg agcactggcc gtggcagggt
    11221 caagcttcag cctggtgaag ataaaggggg tgattatccc gataaggctg ccggtcatca
    11281 ccgccagcac catggtaatt gccaccaccg caatgtactg cggagcgcgc acactggcga
    11341 tcaggcttac accggccgcc atggtaatac ccagcaggaa ggagaccagg aattccctgc
    11401 ccagcagcct gaaccagtct tttgtttcca catcccctat cgccagggat cggatcataa
    11461 gcgtagctga ctgtgcccct gcatttcctc cgctcccaat caatagtggc aggaaaaaaa
    11521 tcagcgatac ggccgattga atcacgcctt cgaaagtagc cagagccgct cccgaaaata
    11581 cattcataaa aaccaggacg atcagccaaa gaattcgttt ctgataaaga aaaaagattt
    11641 tggctttcag cggattgaca atggccgcct ggaatgaacc gaatttgtga aagtcttcgg
    11701 tagtttcctc ttccacaacg tccatcacgt cgtcgaccgt aacaatgccc agcagcaatc
    11761 cgtccgaatc gagcaccggc aatgcgatgc ggtcgtggtc ctgaaatacg cgaatggcca
    11821 cttcctgatc gtcgaacgca ttaagcgata cgaaatgctc gtccataagg tcccggatgt
    11881 gctgcccggg atcggccaga atgatttccc ggatgcggat atcgtcgatc agtttccagt
    11941 tctcatccac aacgtaaatc agattgaggg tttccgaatc cctgccgtac atgcggatgt
    12001 gcttcagcgc ctgttcaacg gtatattcag gccttacagc cacgaattcc ggcgtcatga
    12061 gccttccgat gctgtgctct ggatatccca gcagacgggt ggcaatgatg cgctcttccg
    12121 gcgacaacag ctgtatcagc cgctgtgtaa tgttgcccgg aagctcctcg aaaaacgccg
    12181 tacgatcgtc gggatccagg tcgttgagca agcctgcaac ccggttaaca tttttggcaa
    12241 gcccttctat aatttgctcc tgcgtgtcgt gctccaggta ctggaaggtg tttttcgcct
    12301 gttcccttgg cagcagacgg aacaggatga cgtcgtcctg ctcgggcaac tcatctatta
    12361 tttcagcgat cagcaccgga tccatgtcct caagctcatg cctgagcgtg ctccagtctt
    12421 tatccctgat cagatcttcg aaaaaggtaa tttcggtttc acccattgat tgttctcctt
    12481 acgggtatta actgcaagga gatacggaac ccccatgcag gttagtgttt aatcaggcca
    12541 cacgaatagg ggtcgtcttt catggtcggg aattgtattg tggatgcaaa gatagatttt
    12601 ttcgcgcgaa acggcgtttc atccgggcgg ttttgttgta atttattacg catttttctg
    12661 ccccggttta cgattccact aatttttgaa atgcaatgag ctgtattacg ggcgattatt
    12721 tctttgtatt gcgacaagca acgaattata agggtcggat taaaccgtag ttttgcaaaa
    12781 aacaaaccta tgaacagatt aaacggtatt ttgctggtac atgctgtgct gctttcggtt
    12841 tgcctgcagg cccagcagca ttcggatccg gaacgtgcgc tggtgctcag cggcgataca
    12901 accgcaatga tgcgtgtttt aactacttct catgccgggg atgcagcaat cttaaatgca
    12961 gaatgccggg atctggatcc ttttgatcct atggtgcgtc tgttgtcgca acgtatgttt
    13021 ctgagtatgc gcgattcagc caaccccggg gtgggaatag ccgcccccca ggtgggcatt
    13081 aaacgaaatg ccttctggat tcaacgcttt gataaacagg gatttccttt tgaatttatg
    13141 attaatccgg tcatcctgag ttattctgcc cttaagcgca ggggtggaga aggttgcctt
    13201 tccattcccg gtgtcagggg agaagtgctg cgcagttacg ccattatggt tgaataccgg
    13261 gatttttcag gaggatggca taccgaattg ctcgaagact tcacggccgt aatctttcag
    13321 cacgaatacg accacctgaa agggatactt tttaccgacc gaatggatca acagctcagt
    13381 tttcccgttt accctcttcc tgagggaaca gaactttttc tcggacctcc gggaatgtaa
    13441 ttctatttat tgcccgatgc aataaagtaa aggcaaaccg acagaagcag caggtgagtg
    13501 acctgtactg ccgttacccg gggaacaaat ccgaggtaaa tggtgtatgc aaacagcagg
    13561 ctcagcagaa gtcccgacac tacggtaagt gagggtttgg acgtaaaccc tccggcaaac
    13621 agcaataccc cgaaaagtac gaaggccagg gcaaggtaaa agccttgcga ctggtagttc
    13681 agggcaagga ttgtttgacc gtgctgaagc aaaagccagg tgagcagtgt aatccgaagc
    13741 agccatttgg aggccggaag cagcgatttt accggtttca ttccctttgg tcctgaatgt
    13801 taattacaag tttcagttcg tcgagctgtt cctgtttgat gggtgccgga gcatcgatca
    13861 tcacatcgcg gccggcattg tttttcggga aggcgataaa atcgcgtatg ctgtccgacc
    13921 caccgaaaag agaacacatg cggtcgaagc caaaggcaat gccgccgtgg ggcggagcac
    13981 cgtattcaaa ggcgttcatc aggaaaccaa actgagcctg ggcatcctca tcgctgaaac
    14041 ccaggatggt aaacattttc ttctgcaaat ctttattatg aatacggatg gatcctcctc
    14101 cgatttccac cccgttgatg accaggtcgt aggcattggc gcgcaccttg ccgggatcgc
    14161 tgtcgagcag aggtatgtct tccggtttcg gagaggtaaa cggatggtgc atggcataat
    14221 aacgctgcgt ttcatcgtcc cactccagca gggggaagtc aaccacccag agaggagcaa
    14281 atacttccgg attgcgcagt ccaagcaggt tgcccatttc aaggcgcagt tccgataagg
    14341 ccttgc
//
LOCUS       DAOSDR010000022        13799 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrimonas sp. isolate AMR_MDS_1826
            NODE_1734_length_13799_cov_4.113650, whole genome shotgun sequence.
ACCESSION   DAOSDR010000022 DAOSDR010000000
VERSION     DAOSDR010000022.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863355
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrimonas sp. (wastewater metagenome)
  ORGANISM  Petrimonas sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Dysgonomonadaceae; Petrimonas.
REFERENCE   1  (bases 1 to 13799)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13799)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:10:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,720
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 35
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13799
                     /organism="Petrimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1734_length_13799_cov_4.113650"
                     /isolate="AMR_MDS_1826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2023866"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..367)
                     /locus_tag="PLI30_01700"
     CDS             complement(<1..367)
                     /locus_tag="PLI30_01700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006842663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HOI78297.1"
                     /translation="MEYAFCGTSGLQLPRISLGLWHNFGYTDDFEEARNMIVHAFDQG
                     VTHFDIANNYGPPPGSAESNFGKILKENLASHRDEMIISSKAGYLMWDGPYGNGSSRK
                     YLMASLNQSLKRTGLEYFDI"
     gene            complement(385..2196)
                     /gene="mutL"
                     /locus_tag="PLI30_01705"
     CDS             complement(385..2196)
                     /gene="mutL"
                     /locus_tag="PLI30_01705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005804242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HOI78298.1"
                     /translation="MTDIIHLLPDSIANQIAAGEVIQRPASVVKELMENSLDANAKHI
                     HIIIKDAGRTLVQVIDDGKGMSMTDARMAFERHATSKIKTSEDLFALYTRGFRGEALA
                     SVAAIAHVELKTRRAEDEMGTWLKIAGSKVESQETIFCAVGTTISVKNIFFNVPARRK
                     FLKSNETERRNIFTEIERIALVNPDIEFTVIENDVQTMHLPPANLRQRIVQMQGKGIN
                     QQLIEINEETTLAKIHGYVSTPQFARKGRANQFFFVNNRYIRHPFFHKAVMTAYEPLI
                     SATENPNYFIYFEVEPDTIDVNIHPTKTEVKFENERALWQILMVTVKESLGKFNAIPS
                     IDFDTADAPDIPIFDPSKSTSMPKVNINPDYNPFQPSYGESTRISKPRLDWEQMYRGF
                     ENEGKPMSGSPDADATLFQSNESLVSPKHYQYKEKYILTSVKSGLMIIDQHRSHVRIL
                     FEKYLSQITNRKGISQRVLFPERIELTSSEASSLSEIQEDMEALGFEMSDLGNNSFAI
                     QGIPSELQNVDAVALVRSMIDKNMETGSNIQEQMHEKLALSMANFTAIPAGKSLTEEE
                     MLRMVDQLFACQSPNHTPDGKSIITVISDDEMENKLK"
     gene            complement(2213..2551)
                     /locus_tag="PLI30_01710"
     CDS             complement(2213..2551)
                     /locus_tag="PLI30_01710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI78299.1"
                     /translation="MGMFFFYNNKKPRKFNYTPILYDPDKEERKEQMEKRIRRIREEV
                     AREEGKTVETPREAVNTDFGEAFLSQTKYLKRRKEKEKEGSKPFFTSNLTVVLLIIAL
                     LVIFYFVFLR"
     gene            complement(2611..4233)
                     /locus_tag="PLI30_01715"
     CDS             complement(2611..4233)
                     /locus_tag="PLI30_01715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019541232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OstA-like protein"
                     /protein_id="HOI78300.1"
                     /translation="MKKQTCPNITIKSILFAGVLFVLMLPARPQQPPPTNPNVKIVEL
                     KQADLLYKRESSEAQILKDNVVLYHEGAFMYCDSAYLFEKTNTFEAFSNVRMEQGDTI
                     FVYGDYLHYDGNTRLARLRDNIRMEDRQVTLFTDSLNYDREANLGYYFDGGMLVDEKN
                     ELTSFWGQYDPVSKDALFSDSVKLVNEDYTIFADTLKYNTESKVADILGPSTIVSDSG
                     FIKTSHGWYNTGTDDARLFDRSEIYSSDSTKMLVGDTVFYNRQTGLGEVFGKMYLEDF
                     KQKAILRGNYGTYNEKTEYGMATDSAYAIDYSQSDSLFLHGDTLKMFTDSIYKEIKAY
                     YNVRFYREDIQGVCDSMNYVQKDSALYLLGNPVIWNKSNQILGNRIDIYLNDSTIDKA
                     HVRDYALAIQDREMTGQYNQISGRDLKAFFAEGELRHVLVEGNAESLYYLVEEDSAHT
                     VIGLNKTESAYLSMDFLDDELQKLKLWSSTKAVTTPLSQLKQEESKLKGFMWLDYLRP
                     LHKMDIFRRNERQGSDSEVQAPRRFSRDDAVE"
     gene            complement(4294..5616)
                     /locus_tag="PLI30_01720"
     CDS             complement(4294..5616)
                     /locus_tag="PLI30_01720"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009859571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HOI78301.1"
                     /translation="MANSAASQNNIVDEIVWVVGDEAILKSEVEEYRKDIQMQNQRIE
                     GDPYCFIPEQMAINKLFLDQAKLDSIEVNEANVNRMLEYYMNEYISSTGSAEKLQEYF
                     GKSLRKIREDLRTQLREQQLIQGVQQKHFENVSLTPSEIRKFYNTIPQDSLPFIPTTM
                     EVQIITVEPEVPLKEIDAVKNRLREYTEQINSGKSSFSTLALLHSEDPGTALKGGEMD
                     FQTRAQLVPEFSNVAFALNDPTKVSNIVETEYGYHIIQLIERRGDMGKFRHILLKPKI
                     PQEQLDTALVRLDSIRNGIVNKKITFDEAATFFSADKDTRNNKGIMVNNRSNSQNTGT
                     PRFQLSELNQDIAKVVGEMKVGEMSKPFVMVTDKGKQVAAVVKVTNRNDGHKANINND
                     YQIIKQMAENDRRQELMDDWLQKKIETIYVRIDPNWKGCDFKYKGWLK"
     gene            complement(5849..6862)
                     /locus_tag="PLI30_01725"
     CDS             complement(5849..6862)
                     /locus_tag="PLI30_01725"
                     /inference="COORDINATES: protein motif:HMM:NF016777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HOI78302.1"
                     /translation="MEYYKSSDFKSVKKVDAHLHINTDDGRYLEFATLQNFSILSPNV
                     DAGTPVEKQLSIAAVHRQNYPGNFAFLGTFTVDSFGTSGFVRSVIGQIDRAIEQGASG
                     VKIWKNIGMSLKDSNGRYVMADDPIFDPIFQYLTDKKIPLMAHLGEPRNCWLPLEQMT
                     DSGDYRYFKNHPEYHMLLHPEAPSYEDQINARDNVLKKFPGLTFVASHIASLEWNLDE
                     VAERLDRYPSMSIDLSARMGHVQRQSVADYEKVRDFFIRYQDRILYGIDITISEGGDR
                     FDTVSSEMLRKWESDWAYLATDSIQVIENISGDIRGLHLPKTVIDKVYYENVNRYFSA
                     FEK"
     gene            7055..7393
                     /locus_tag="PLI30_01730"
     CDS             7055..7393
                     /locus_tag="PLI30_01730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019131287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMR family transporter"
                     /protein_id="HOI78303.1"
                     /translation="MNWIFLIVGGLFEAIFAFSLERMSASTGKTAVMWFLSFLISVSI
                     SMFLLFKAISGENAVAVGTGYAVWGGIGAVFTVLAGILFLGESTAFWRIFFLCTLVLS
                     VIGLNLVGKH"
     gene            complement(7447..10164)
                     /gene="ppdK"
                     /locus_tag="PLI30_01735"
     CDS             complement(7447..10164)
                     /gene="ppdK"
                     /locus_tag="PLI30_01735"
                     /EC_number="2.7.9.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009126652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050242 - pyruvate, phosphate dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, phosphate dikinase"
                     /protein_id="HOI78304.1"
                     /translation="MKKKRVYTFGNGQAEGRADMKNLLGGKGANLAEMNLIGVPVPPG
                     FTITTEVCTEYNQLGKDYVVDVLKPEVEKAIKNIEKLMGAKFGDRENPCLVSVRSGAR
                     VSMPGMMDTVLNLGLNDDAVEGIAKKSGNERFAWDSYRRFVQMYGDVVLGMKPQSKED
                     IDPFEEIMEEMKQAKGVELDNELTVDDLKELVKRFKSAVKKRTGNDFPESAWEQLWGA
                     VCAVFDSWMNERAILYRKMNNFPEEWGTAVNVQAMVYGNMGKTSGTGVAFTRDAATGE
                     DIFNGEYLIDAQGEDVVAGVRTPQEITLEGSRRWAKLQGVSEEERAAKYPSLEEALPE
                     CAKELIETQQKLEDYFKDMQDLEFTIQDGKLWLLQTRSGKRTGAAMIKIAIDMLHQGY
                     IDEKTALKRCDPEKLDELLHPVFDKKALAAAKPVTNGLPASPGAAAGQVVFHADEAEE
                     WAKEGKKVILCRIETSPEDLRGMATAQGILTARGGMTSHAAVVARGMGKCCVSAANGV
                     VINYKERKMTINGKVFNEGDWISLNGTTGNVYEGKIQTQDAELGPDFLELMKIADKYT
                     RMNVRTNADTPRDAKVARNFGATGIGLCRTEHMFFEEDKIIPMREMILAKNEEGRRKA
                     LEKLLPLQKKDFKGIFEAMDGYPVTVRLLDPPLHEFVPHDEKGQMEMARVMDISYSDI
                     HERVESLMESNPMLGLRGCRLGNLYPEITEMQTRAIIEAALELKREGIKAIPEIMVPL
                     TGIVYEFQAQKEIIEKTIQQVFSENSDSIEYKIGTMIEIPRAALTAHKIAKEADFFSF
                     GTNDLTQMTFGYSRDDVAKFLPLYIDKGILKQDPFAVLDQNGVGQLVSMAVQNGRAVK
                     PSLKCGICGEHGGEPSSVKFCHRVGLNYVSCSPFRVPIARLAAAQAAIE"
     gene            complement(10269..11108)
                     /locus_tag="PLI30_01740"
     CDS             complement(10269..11108)
                     /locus_tag="PLI30_01740"
                     /inference="COORDINATES: protein motif:HMM:NF025494.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4296 domain-containing protein"
                     /protein_id="HOI78305.1"
                     /translation="MNRKVAAYLLTSVILSILFSFCNRPKEVLNRKDMEKLMYDIYVA
                     EAMIENDYQGFDTPEKKEALIRDVFRKHQTTQAQWDTSLSWYSDRIDIYLKMNDSVKA
                     RLQRRQRASEQVMNRQLQQEQSLTARSYSPSYIPENYSFDETNPKNGFRFRMDTTDIA
                     QKINSSGFDFGFDVIGIPARSKPDLRAVLMLEYNDTVIYHSELIVENRSYLLHGQKYI
                     PSDTIRGITGFVRLQDTIGMFRNIRLNNIFLGHPNDSVQPAQRNVSAPQERILMDEET
                     GTQ"
     gene            complement(11098..11784)
                     /locus_tag="PLI30_01745"
     CDS             complement(11098..11784)
                     /locus_tag="PLI30_01745"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019541228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein signal peptidase"
                     /protein_id="HOI78306.1"
                     /translation="MNSSTKKGLIAVSVIFLVLLVDQVSKIWVKTHMALYDMIHITDW
                     FKIYFVENNGMAFGIEAIGKLFLSLFRIIAVGAIFVYLLRIVRENYKIGFIACIALVL
                     AGASGNIIDSVFYGVFFQASYPGHVASVVPFGEGYASLLHGKVVDMFYFPLIEGTYPD
                     WFPVMGGKDFLFFRFIFNVADSAISVGVVLLLVFYRKTLSYSLLSKKDKEKFDRERQE
                     QLEKKAVCES"
     gene            complement(11881..12261)
                     /locus_tag="PLI30_01750"
     CDS             complement(11881..12261)
                     /locus_tag="PLI30_01750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005641525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA C4-type zinc finger protein"
                     /protein_id="HOI78307.1"
                     /translation="MSEKTRYSDEELEEFRAIINEKLEVARQEYENYRAAVTNADGND
                     TVDTSPTYKVLEEGASTLSKEEAGRLAQRQMKFIQNLQAALIRIENKTYGVCRETGKL
                     IPKERLRAVPHATLSIEAKQGKRK"
     gene            complement(12435..>13799)
                     /locus_tag="PLI30_01755"
     CDS             complement(12435..>13799)
                     /locus_tag="PLI30_01755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011966017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5915 domain-containing protein"
                     /protein_id="HOI78308.1"
                     /translation="GNKMSKRLGNAVDPFEAIEKFGSDPLRWYMVTNAAPWDNLKFDI
                     DGVEEARRKFFGTLYNTYSFFALYANVDDFHNQYPPIALAKRPEIDRWILSLLNSLVK
                     EVDGDYASYEPTKAGRAINDFVNDNLSNWFVRLNRKRFWGGEMTEDKMSAYQTLYQCL
                     VTVAKLMAPIAPFYADRLYLDLVKVTGSERFESVHLADFPAVDESAVDVALEEQMYLA
                     QTASSIVLALRRKVNLKVRQPLTKIMIPVADEKQRRNIEAVQALILSEVNVKELEFVD
                     SANDMLVKRVKPDFKKLGPKYGKIMKQLAACIQEMQRDEINKLEKNGCFAFRIEGQEA
                     VIDLADVEVISEDIPGWLVANEGQLTVALDITVTDELRKEGIARELVNRIQNLRKSKD
                     FDITDRIAVRFSSNPLFDSAVREFSEYIKTQVLADSIEIETLVFEDEIEIDDEKLTID
                     VRKQ"
BASE COUNT         3410 a         3318 c         2976 g         4095 t
ORIGIN      
        1 agatatcaaa atattccaat cccgtacgtt ttaaactctg gttcaggctc gccatcaggt
       61 atttcctgga ggatccgtta ccgtaggggc cgtcccacat cagatatccg gctttggacg
      121 aaataatcat ttcatcgcgg tgcgaggcca ggttttcttt cagtatcttt ccaaaattgg
      181 attcggccga acccggaggt ggaccgtaat tgttggcaat atcaaaatgg gtcactccct
      241 gatcaaaggc gtggacaatc atgtttcgcg cttcttcaaa atcgtcggta taaccgaagt
      301 tgtgccacaa tccgagcgaa atccggggca actgcaagcc gctcgttcca caaaatgcgt
      361 attccataag tcaaagaatt taaattattt cagtttattt tccatctcgt catcagaaat
      421 gacggttatt attgattttc cgtcgggtgt gtgattgggt gactggcagg cgaacagctg
      481 atcgaccatc cgcagcattt cttcttccgt aagtgatttt cctgcgggaa tagccgtgaa
      541 atttgccatc gacaacgcca gtttttcatg catctgctcc tggatgttgc tgccggtctc
      601 catgttttta tcgatcatgg aacgcaccaa tgccacagca tcaacgtttt gtaattccga
      661 cggaatacct tgaatggcaa aagaattatt tcccaagtca ctcatttcaa agcccagtgc
      721 ctccatatct tcctgaattt ctgaaagaga agaagcttcg gaagaggtca gttcgattct
      781 ttcgggaaac agcacccgct gagaaatacc ctttctgttg gttatctggg aaaggtactt
      841 ttcaaaaaga atacgaacat gtgagcgatg ctggtcaata atcatcaacc ccgattttac
      901 cgacgtaagg atatatttct ccttgtactg gtaatgtttg ggtgaaacta acgattcgtt
      961 ggactgaaaa agggttgcgt ccgcatcggg tgaaccggac attggctttc cttcattttc
     1021 aaatccccgg tacatctgtt cccagtcgag cctgggtttc gaaatccggg tactctctcc
     1081 gtatgaaggt tgaaaaggat tgtaatcagg attgatattc acttttggca tcgaagtaga
     1141 cttcgacggg tcaaaaatag gaatgtcggg tgcatctgcc gtatcaaaat ctatgctggg
     1201 gatagcattg aattttccga gtgattcctt taccgttacc atcaggattt gccataaggc
     1261 acgctcgttc tcaaatttca cttcggtttt cgtgggatgg atattcacat ctatcgtatc
     1321 cggctccact tcgaaataaa tgaaataatt gggattctcg gtagccgaaa tcaacggctc
     1381 gtaagcggtc atgacggctt tatgaaaaaa aggatgacgg atataacggt tattgacgaa
     1441 gaaaaactga ttggctctcc cctttctggc gaactgcggc gttgaaacat atccgtgaat
     1501 tttagccagg gtggtttctt cgtttatttc tataagctgc tgattgatgc cctttccctg
     1561 catctgcacg atgcgttggc gcaggtttgc cggagggagg tgcatcgtct gcacatcgtt
     1621 ctcgataacc gtaaactcaa tgtccgggtt cacaagggca atacgctcta tttccgtgaa
     1681 gatattccgt cgttcggtct cgttcgattt caggaacttg cgacgggccg gcacattaaa
     1741 gaatatgttt tttacagaga ttgtagttcc cacagcgcaa aagatggttt cctggctttc
     1801 taccttcgat ccggcaatct tcaaccatgt ccccatttca tcctccgcac gccgggtttt
     1861 aagttctacg tgcgcgatag ccgctacgga tgcgagtgct tcaccacgaa aaccccgggt
     1921 gtacaaggca aagagatctt cagaggtttt aatctttgat gtggcgtgac gttcaaatgc
     1981 cattcgggca tcggtcatac tcataccttt gccatcatca atgacctgaa cgagtgttct
     2041 cccggcatct ttaataatga tgtggatatg tttggcgttg gcatcgagcg agttttccat
     2101 cagttctttc actacagatg caggccgttg aataacctca ccggctgcga tctgattggc
     2161 tatggagtcc ggtaacagat gaattatatc cgtcatgttt ttgttgtagc gtttagcgca
     2221 ggaacacaaa atagaagatt acaagcaacg caataatcag caagacgaca gtaaggttgc
     2281 tggtaaaaaa aggcttgctt ccctcttttt ctttttcttt tctgcgtttc aaatacttgg
     2341 tctgggagag gaacgcttca ccaaagtcag tgttgacagc ctcccgcggt gtttcgactg
     2401 ttttgccctc ttctcgggcg acctcttccc ggatcctccg gattcttttt tccatctgtt
     2461 cttttcgctc ctccttgtct ggatcgtaaa gtatgggtgt ataattaaat tttcgaggtt
     2521 ttttattgtt gtaaaaaaag aacattccca tagctgtcaa ttttatcgtt acacaaaatt
     2581 aaaaaaagaa tagtaataaa caaaagccat ttattcaaca gcatcgtccc gtgagaatct
     2641 tctcggagcc tgcacttcgc tatcggagcc ctgtcgttcg ttgcgacgga agatatccat
     2701 tttgtggagc ggtctcaagt aatcgagcca cataaaccct tttaatttcg attcctcctg
     2761 tttcagttgg ctcaacgggg tagttacagc cttagtggat gaccatagtt ttagtttctg
     2821 caactcatcg tccaggaagt ccatggacag gtaggcgctt tcggttttgt tcaatcctat
     2881 aacggtgtgc gcactatctt cttccaccag gtaataaagc gattcggcat tcccctccac
     2941 caaaacgtgc ctgagttccc cttctgcaaa aaaggctttc aaatcccttc cgcttatctg
     3001 gttatattgt cccgtcattt ctctgtcctg gatggcaagg gcataatctc tcacgtgtgc
     3061 tttatcaata gtactgtcgt tcagataaat atcgatacgg ttgccgagaa tctgattgct
     3121 tttattccaa atgaccggat tgccgaggag gtacaacgcg gaatcttttt gaacgtagtt
     3181 catggaatca caaaccccct gaatatcttc cctataaaac cggacgttgt aatacgcctt
     3241 gatctccttg taaatggagt cggtaaacat tttcagcgtg tctccatgaa gaaaaaggct
     3301 gtcgctctgt gaataatcaa tagcgtaagc cgagtcggtg gccattccgt actcagtttt
     3361 ctcgttatac gttccgtagt tcccgcgcag aatagctttc tgcttaaaat cctcaaggta
     3421 catcttgcca aaaacctccc ccagaccggt ttgccggtta tagaaaacgg tgtctcccac
     3481 aagcatcttt gtgctgtcac tgctgtaaat ttctgatcgg tcaaacaacc gtgcgtcgtc
     3541 agtcccggtg ttgtaccacc cgtgtgatgt tttgataaac ccgctgtctg agacaatggt
     3601 tgaaggaccc aggatatcag cgaccttact ctccgtgttg tatttcagtg tatcggcgaa
     3661 tatagtatag tcctcattga ccagttttac gctgtcgctg aacagggcgt ccttgcttac
     3721 cggatcatac tgtccccaaa aggaggtaag ctcattcttt tcgtccacca gcattccccc
     3781 gtcaaagtaa taccccaggt ttgcctcacg gtcgtagttc aggctatctg taaagagcgt
     3841 aacctgacgg tcttccatac ggatattgtc ccgaaggcgt gccaggcggg tattcccgtc
     3901 gtaatgcagg taatcaccgt acacgaaaat ggtatcgccc tgttccatcc ggacattgct
     3961 gaaagcttcg aacgtgttcg ttttttcgaa caggtaggcg ctgtcgcaat acataaaagc
     4021 accttcgtga tacaagacca cgttatcctt caagatttga gcttcggaac tttcccgttt
     4081 ataaagcaaa tcggcttgct tcagttcgac aatcttcacg tttgggttgg ttggtggcgg
     4141 ttgttgcgga cgggcgggta acataagcac aaaaagcaca cccgcgaaga gtatgctttt
     4201 gatggtaata ttggggcacg tttgcttttt catccgaaaa acgttgttcg attgttgcgt
     4261 tgaatatttc accgccacaa cgaattaagc ctattacttc agccagcctt tatacttaaa
     4321 atcacaccct ttccagttgg gatcaattct aacgtagatg gtttctattt tcttctgcaa
     4381 ccagtcgtcc atcagttcct ggcgacggtc attttcagcc atttgcttaa tgatttggta
     4441 gtcgttgttg atgttggctt tgtgcccgtc gttccggttg gtgactttca ccacggctgc
     4501 tacttgcttg cccttatcgg taaccataac aaagggttta gacatttccc ctactttcat
     4561 ttcccctacc accttggcaa tgtcctgatt aagctcactc aactggaaac ggggagttcc
     4621 cgtgttttgt gaattggacc ggttgttgac cataattccc ttgttgttcc gagtatcttt
     4681 atccgccgaa aagaaagtag cagcttcgtc aaacgtgatt tttttattga caattccgtt
     4741 tcggatggag tcgagccgga caagagcagt gtccaattgt tcctgtggaa ttttcggctt
     4801 cagcaaaatg tggcggaatt tacccatatc ccctcttcgt tcgataagtt gtatgatgtg
     4861 atatccatat tccgtttcaa ctatattcga aacttttgtc ggatcgttga gagcaaatgc
     4921 tacattcgaa aactcgggaa caagttgcgc ccttgtctgg aaatccattt cccctccctt
     4981 gagtgccgtc cccgggtctt cggagtgcaa caaggccagt gtggaaaaag agctttttcc
     5041 cgagttgatc tgctcggtat attcacgcag cctgttcttc actgcgtcta tctctttcaa
     5101 aggcacttcg ggttcaacgg tgattatctg tacttccatg gtggtaggga taaacggcaa
     5161 gctgtcctgc ggaatcgtgt tgtaaaactt ccggatttca gaaggggtca gcgatacatt
     5221 ctcaaagtgt ttttgctgca caccctgtat cagctgttgc tcgcgtaact gggtccgaag
     5281 gtcttcgcgg attttcctca ggctcttacc aaaatactct tggagttttt ctgccgatcc
     5341 cgttgatgaa atgtactcgt tcatgtaata ttccagcata cggttcacgt tggcctcatt
     5401 cacctcgata ctgtcgagct ttgcctggtc gagaaacaac ttgtttatag ccatctgctc
     5461 agggatgaaa caatatggat cgccctcgat acgctggttc tgcatctgaa tgtccttccg
     5521 gtattcctcc acctcggatt taagtatggc ttcatcgccc accacccaaa cgatctcatc
     5581 gacaatgtta ttttgagaag cagcactatt cgccatcaac gtaaaaacga caagcgataa
     5641 tagtattttt gcagaaaatt tcatttttta caggtttttt gaaaagttga atgtaaaagt
     5701 aattataata aaattatcgg gcaacaaatc aattattaaa agatacaaaa tcatctgtcg
     5761 acggctgtct caggaaatct gcaaggcaaa gtaacaccct ccgtgcggga ttacttactc
     5821 acgggccggc cgacagctgt tatccggctt atttctcaaa agcactgaaa taccggttca
     5881 cattttcata atatacctta tcgattacgg ttttgggcaa gtgaagccct cggatatcgc
     5941 cactaatgtt ctctataacc tgtatggaat ccgttgccag gtaagcccaa tctgattccc
     6001 atttccggag catttccgac gaaacagtat cgaaacggtc accgccttcg ctaatcgtta
     6061 tatcaatgcc gtataaaatc cgatcctggt accggataaa aaaatcgcgt actttttcat
     6121 agtcggcaac cgattgacgc tgaacgtgcc ccattcgtgc cgataaatcg atgctcatcg
     6181 acggatagcg atcaagcctt tcggcaactt catccaggtt ccactccaag ctggcgatat
     6241 gagacgccac aaaagtgagg cccggaaact tcttcagtac attatcgcgg gcgttgatct
     6301 gatcttcata tgagggagct tcggggtgta acaacatatg atactcaggg tgattcttga
     6361 aataacggta atcgccggaa tcggtcatct gttctagcgg gagccagcag ttgcgcggtt
     6421 cgccgaggtg agccatcaaa ggaatcttct tgtctgtcag gtattgaaat atggggtcaa
     6481 agatcggatc atccgccatg acatacctgc cgttgctgtc tttcaggctc atcccgatgt
     6541 tcttccatat tttaactccg gaagcgccct gctcaatggc acggtcaatc tgtccgatca
     6601 cgctccggac aaaaccggaa gtaccaaaac tatctacggt gaatgtcccc agaaaagcaa
     6661 agtttccagg ataattttgc cggtgtacgg ctgcaatact tagctgtttt tcgacaggtg
     6721 ttccggcatc aacgtttgga gaaagtatgc tgaagttttg cagtgtggca aattccaggt
     6781 accgtccatc gtccgtattg atgtggagat gtgcatctac ctttttcacg gatttaaaat
     6841 cactactttt gtaatactcc attttttttt gacaggaaac ggacaaagcc aaacaagaaa
     6901 aaacgaacaa caatactttc atttaaatgg ggagtgtttg tctaacgggt tgaatagttg
     6961 tccaaaatta gtatttattg ctaaatagag caaggcttta tccagaaaaa acataaaagg
     7021 tgtatatttg caccctttac attttacaac tatgatgaat tggatttttc ttattgttgg
     7081 cggattgttt gaggccattt ttgctttcag tcttgaaagg atgtcggcaa gtacaggcaa
     7141 aacggccgtt atgtggtttt tgtcctttct gattagcgtc tccatcagta tgtttctact
     7201 tttcaaagcc attagcggtg aaaatgccgt tgccgttgga acaggttacg ccgtgtgggg
     7261 tggaataggt gcagtgttta cggtattggc aggaattctt ttcctgggag aatccacggc
     7321 cttctggcgg attttttttc tttgcacact ggttttgtcg gttatcggac tgaatttggt
     7381 gggcaagcat taaaaagtaa agacgcgact tatcgcgtct ttacaaaatt tatcttaccg
     7441 gagattttac tcaatcgcgg cctgggctgc tgccaaacgt gcgataggca cccggaacgg
     7501 agaacaggag acatagttga gccctacacg gtgacagaac ttcaccgatg aaggttcacc
     7561 cccgtgctca ccacaaatac cgcatttaag tgagggtttc actgcacggc cattctgtac
     7621 ggccatactt accagttgtc ctaccccgtt ttgatcgaga acagcaaacg ggtcttgttt
     7681 caggatccct ttatcaatat ataacggcag gaatttagcc acatcatcac ggctatagcc
     7741 gaaagtcatc tgtgtcaggt cgttcgtacc aaacgagaag aagtcggctt ccttggcgat
     7801 cttgtgagcg gtgagtgcag cacgggggat ctcgatcatt gtcccaatct tgtattcgat
     7861 ggaatcactg ttttcgctga atacctgttg gatagttttt tcaataatct ctttctgtgc
     7921 ctggaactca tacacgatac ctgtcagcgg aaccatgatt tccggaatgg ccttgatgcc
     7981 ttcccttttc agttcaagcg cagcttcaat gatagcccgg gtttgcattt ccgtgatttc
     8041 gggatacaag tttccaaggc ggcaaccgcg cagcccaagc ataggattcg attccatcag
     8101 gctttccaca cgttcgtgta tatcgctgta agaaatatcc attaccctcg ccatttccat
     8161 ttgccctttt tcatcgtggg gaacaaattc gtgcaaagga ggatccaaca aacggacagt
     8221 cacgggatat ccgtccatag cttcaaaaat tcctttgaaa tcttttttct gcagggggag
     8281 cagtttttcc agcgctttac gacggccctc ttcatttttg gccagaatca tttcacgcat
     8341 gggaataatc ttgtcttctt cgaagaacat gtgttcggtg cggcacaaac cgattccggt
     8401 agccccgaaa ttacgtgcta ctttcgcatc ccgaggtgtg tcggcattgg tccttacgtt
     8461 catacgggta tacttgtctg caatcttcat cagttcgaga aaatcgggac ccaactcagc
     8521 atcctgcgtc tgtatttttc cttcgtaaac atttccagta gtaccgttta acgaaatcca
     8581 atccccttca ttaaacactt tcccgttaat ggtcattttc ctttccttgt agttgataac
     8641 cacaccgttt gctgccgaca cgcaacattt ccccataccg cgtgccacaa cggcagcgtg
     8701 agaagtcatt cctccgcggg cggttaaaat cccctgtgct gtggccatac cgcgcaagtc
     8761 ttcgggcgac gtttcgatgc ggcagagaat cacttttttt ccttctttag cccattcttc
     8821 agcttcgtcg gcatggaaga caacttgtcc tgcggcagcc cctggcgatg cggggagccc
     8881 gttggttacc ggtttggccg ctgccaatgc ttttttatcg aaaacagggt gtagcaactc
     8941 gtctaatttt tcgggatcgc agcgtttcag tgccgttttt tcatcgatat aaccctgatg
     9001 aagcatatcg atggctattt tgatcatggc agccccggta cgtttaccgc tgcgggtctg
     9061 caacaaccac agcttaccgt cttgtatggt aaactcgagg tcctgcatgt ctttgaagta
     9121 gtcttcaagt ttctgctgcg tttcaatcag ttctttggca cattcgggca atgcctcttc
     9181 gagtgaaggg tacttagcgg cacgttcttc ttccgatacc ccttgcagtt ttgcccaacg
     9241 gcgtgaacct tcgagtgtta tttcctgcgg tgtgcgcaca cctgccacca catcttctcc
     9301 ttgtgcgtct atcaggtact caccgttaaa aatatcttca ccggttgcag catcgcgggt
     9361 gaaagccact cctgttccgg atgtcttacc catatttccg taaaccatgg cctgcacatt
     9421 aacagcggtt ccccactctt ccgggaaatt attcattttc cgataaagga tggcgcgttc
     9481 gttcatccaa ctgtcaaaaa cggcgcagac ggctccccaa agctgctccc atgcactttc
     9541 gggaaaatca tttcctgtac gttttttcac ggcagatttg aaacgtttta ccaattcttt
     9601 caggtcatcg acagtcagtt cgttgtcgag ctccactcct ttggcttgtt tcatctcttc
     9661 cattatttct tcgaacggat cgatatcctc tttgctttgt ggcttcattc ccaacaccac
     9721 gtcgccatac atctgcacaa aacgacgata agaatcccac gcaaaacgtt cgttccccga
     9781 ttttttggct attccttcca cggcatcgtc atttaatcca agattcagga ccgtgtccat
     9841 catgccaggc attgaaaccc gggcgccgga gcgcacagaa accaaacaag gattttccct
     9901 gtcaccgaac ttagctccca tcagcttttc gatgttttta attgcttttt caacctcggg
     9961 cttcaacaca tctaccacat aatctttgcc aagctggttg tattcggtac acacttcggt
    10021 ggtaatggta aatcccgggg ggacgggcac accgatcagg ttcatttcgg caagattagc
    10081 gcctttccct ccaagcagat tcttcatatc tgcacgaccc tcggcctgtc cgttgccaaa
    10141 ggtgtaaact ctcttcttct tcatctttcg aaaaatttat ttgttaatca ttaaatgttg
    10201 gattctgtgc tgtaattttc tgcaaatgta gtaaaaaatt taatattaga tcgtgtagga
    10261 attcgggatt actgcgtccc cgtttcctca tccatgagta ttctctcctg aggtgccgac
    10321 acgttgcgtt gggcgggttg gacagaatcg tttggatgtc ccaaaaagat attattcagc
    10381 cgaatatttc taaacatacc gatggtgtct tgcaacctta caaacccagt tattccccta
    10441 attgtatcac tggggatata cttttgtccg tgcaacaagt agctcctgtt ctcaacaata
    10501 agctcggagt gataaataac cgtatcgttg tattcgagca tcaatactgc ccgcaaatct
    10561 ggtttcgaac gagcggggat accgataacg tcgaaaccga aatcgaaacc gctgctattt
    10621 atcttttgag caatgtcagt tgtgtccatc cggaaacgga aaccgttttt cggatttgtt
    10681 tcatcaaaag agtagttttc aggaatgtag gaaggcgaat aagaacgggc ggtaagtgat
    10741 tgctcttgtt ggagctgacg attcatgacc tgttcgctcg ctctctggcg gcgttgcagg
    10801 cgcgccttaa cggaatcatt cattttcagg tagatatcta ttctgtctga ataccaggaa
    10861 agggaggtat cccactgcgc ctgcgttgtt tggtgtttcc tgaaaacatc gcggataagc
    10921 gcctcctttt tttccggtgt gtcgaagccc tgataatcat tttcaatcat tgcttcggca
    10981 acgtaaatat cgtacatcag tttttccata tctttcctgt tcagcacttc tttcggcctg
    11041 ttgcaaaaag agaacaaaat gctcagaatt accgaagtca ggagatatgc agcaacttta
    11101 cgattcacaa acagcttttt tttccaattg ttcctgcctt tcacgatcaa acttctcttt
    11161 atcttttttc gagagaagtg aataggaaag tgtttttcga taaaaaacca acaacaatac
    11221 aacgcctacg gatatggctg agtcggcaac gttaaagata aagcgaaaaa acaagaagtc
    11281 tttgccgccc atcaccggaa accaatcggg atatgtcccc tcaataagtg ggaaataaaa
    11341 catatccacc actttcccgt ggagcaggga agcatatcct tcaccaaaag gcactacaga
    11401 ggcaacatgt cccgggtaac ttgcctggaa gaatactccg tagaaaacgc tgtcgataat
    11461 gtttcccgat gcgccggcca acaccagtgc gatgcaggca ataaagccaa tcttgtaatt
    11521 ttctcgaaca atccggagta aataaacaaa aattgctcct acggctatta ttctgaaaag
    11581 ggaaagaaac aatttgccta tggcttctat accgaatgcc atgccgttgt tttccacgaa
    11641 atatatttta aaccaatcgg ttatatgaat catatcgtag agagccatgt gggttttcac
    11701 ccagatttta ctaacttggt cgacgagtaa aaccaagaag atgactgaaa cggcaataag
    11761 tccttttttt gtggagctgt tcattcgggt agccatttgt acgtaattac accggggaca
    11821 gaaatacttc tttcctgtcc agtgatcatt cgtgttgtca ttgaaatcat tagctagcgg
    11881 ctattttctt tttccttgtt tagcttcgat gcttaatgtt gcgtggggca ctgcacgaag
    11941 acgttcttta gggattagtt taccggtctc acgacacaca ccgtaggttt tgttttctat
    12001 acgtattaat gctgcctgta agttttgaat gaacttcatc tgacgttgag ccaaccgtcc
    12061 ggcctcctct ttcgataacg ttgaagcgcc ctcttccaac accttgtaag taggtgacgt
    12121 atccaccgta tcgttaccat ccgcattggt gaccgctgcc cggtagtttt cgtactcctg
    12181 cctggctacc tcaagttttt cattaatgat ggcccggaat tcttccaatt cctcatcaga
    12241 gtatctcgtc ttttcactca taacagtaaa tattttataa tttatttcaa ttgtttttcc
    12301 taacgtttag tatcgatcaa ttaataatcg gcaacggtgt tgttttatgt caaaataatc
    12361 ttacctgtag ttgttttcgc aaagataaca aaaattagtt accgatatat caatcataaa
    12421 cgcaaatagt ccgactattg tttacgtaca tcaatagtta atttctcatc atcaatctct
    12481 atttcatctt caaaaaccag tgtttctatt tctatagagt cggcaagcac ctgagtcttg
    12541 atatattcgc tgaattccct gacggcactg tcgaacaacg gatttgacga aaaccggaca
    12601 gcgatgcggt cggtaatatc aaaatctttc gacttgcgta aattctgaat acggtttacc
    12661 aactcgcggg caatcccttc tttgcgcagt tcatcagtta cggtgatatc caacgcaacg
    12721 gtcaactgtc cctcgttggc caccagccag cctgggatat cttccgaaat aacttccacg
    12781 tcagccagat cgattacggc ttcttgtcct tctatccgaa atgcaaaaca accgtttttc
    12841 tccaacttat ttatctcatc ccgctgcatt tcctgaatgc atgcagcaag ttgcttcatt
    12901 attttcccgt atttaggccc aagtttcttg aaatcgggtt tcacccgctt cactagcata
    12961 tcgtttgccg aatctacgaa ctccagttct ttcacgttca cttcactaag aataagagcc
    13021 tgaaccgctt caatgtttct gcgctgcttt tcatctgcga cggggatcat gattttcgtc
    13081 aacggctggc gaacttttaa attcactttt cgccgcaacg ccaacacaat agatgatgct
    13141 gtttgcgcca ggtacatttg ctcttcgagg gcaacatcga cagccgattc atcgacggcg
    13201 gggaaatcag ccagatgcac cgactcaaaa cgttcgctac cggttacttt caccaaatcc
    13261 aggtaaagcc tgtcggcata gaacggagcg atgggagcca tcagcttcgc taccgtaacc
    13321 aagcattgat aaagggtttg ataggctgac attttatcct cggtcatctc gcctccccaa
    13381 aaacgtttgc ggtttaaacg gacaaaccaa ttgctgaggt tgtcgtttac aaagtcgttt
    13441 attgctcttc ctgctttggt aggctcgtag ctggcatagt ccccgtccac ctctttaacc
    13501 agggagttca ggagcgacag gatccaccgg tcaatctcgg ggcgtttcgc cagtgcaatc
    13561 gggggatatt gattgtgaaa atcgtccacg ttcgcataaa gggcaaagaa ggaataggta
    13621 ttgtacaatg ttccgaaaaa tttgcggcgg gcctcttcca cgccgtcaat atcgaatttc
    13681 aggttatccc agggtgcggc attggtaacc atgtaccagc ggagcgggtc ggaaccgaac
    13741 ttctctatag cttcaaacgg gtcaaccgca tttcccaatc gtttcgacat cttattgcc
//
LOCUS       DAOSDR010000023        13623 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrimonas sp. isolate AMR_MDS_1826
            NODE_1762_length_13623_cov_4.274985, whole genome shotgun sequence.
ACCESSION   DAOSDR010000023 DAOSDR010000000
VERSION     DAOSDR010000023.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863355
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrimonas sp. (wastewater metagenome)
  ORGANISM  Petrimonas sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Dysgonomonadaceae; Petrimonas.
REFERENCE   1  (bases 1 to 13623)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13623)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:10:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,720
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 35
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13623
                     /organism="Petrimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1762_length_13623_cov_4.274985"
                     /isolate="AMR_MDS_1826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2023866"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1205
                     /locus_tag="PLI30_01760"
     CDS             <1..1205
                     /locus_tag="PLI30_01760"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019541035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006799 - polyphosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="polyphosphate kinase"
                     /protein_id="HOI78309.1"
                     /translation="SGRYLNLADLMKLPNPVGDRLRQTLPQPLRVPELEASNSILRVL
                     RRQDVLLHFPYQSFNYLLRFLLQAAFDPKVLEIKVTQYRVAENSAVINSLISAAKNGK
                     KVTVFVELKARFDEENNYLSSELMQQAGVKIIYSLPGLKVHAKLAYVRKRSNDPDDPI
                     KGYAYLGTGNFNEKTARIYSDKGLLTSNKEIIRDIDEVFRVLEGKPYMHDFRHLLVTQ
                     FNMLSAIHQMIEQEITEVESGREGYIVLKMNSLEDKGMINALYRASEAGVKVDLIIRG
                     ICCLIPNQPYSKNIRVTRIVDAYLEHARVWYFLNGGKETIYLTSADWMQRNLHRRIEV
                     AFPVYSEPLKRQIIDILKIQLEDNQSAVWVNEHLDNVFKRDTASPDNTPIRAQQAIHD
                     YLKQSTGQ"
     gene            complement(1237..2559)
                     /locus_tag="PLI30_01765"
     CDS             complement(1237..2559)
                     /locus_tag="PLI30_01765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008776878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HOI78310.1"
                     /translation="MNEKNSKRRYTVPFIVIVIIAVSALIPMDKSTEFSSLAINNADT
                     AWMLIASALVFLMTPGLAFFYGGMVRYKNLVSTLIQSFITLGIISVIWVLIGFSLAFG
                     DSLGGFIGNPASFFMFNNVGLQPNPAFSATIPFALFAVFQLKFAIITPALITGGVAER
                     IRFSSLMVFIVFFSIFIYAPLAHWTWHPEGFLRKWGVLDFAGGTVVHISAGFAALSGA
                     IFLKKRKDGGTDNPTNIPYILLGTGLLWFGWFGFNAGSALAANELAVKAFLNTNLASA
                     TAMVTWMLTDGARGKKRSVVGAAVGAVVGLVAITPSAGFVTVGQSIFIGCTASLVSYF
                     ATRLKSRSSIDDTLDVFPCHGIGGITGMILTGVFAENVGLIYGDPTVFLYHLLALLIV
                     GAFTFGGSFLLYKLTDLILPMRVTEIQEERGLDITQHGELATDVPSSY"
     gene            complement(2814..4949)
                     /locus_tag="PLI30_01770"
     CDS             complement(2814..4949)
                     /locus_tag="PLI30_01770"
                     /inference="COORDINATES: protein motif:HMM:NF025010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SurA N-terminal domain-containing protein"
                     /protein_id="HOI78311.1"
                     /translation="MATLQKIRDKGVFLVAIIGIALLAFILGDLFTSGNTLFAKSRDK
                     AFVVNGQVISTQEFADRITEWESFQKMISGQTSLDENTSQQIREAVYDQMVRERMLDN
                     EAAKLGLSVSKTEINDLVHGETISPLLQQLPFFLDPQTGMFDREGLMQFLSTVNAPVE
                     SLQPEERAVVEQYKSMWLFIENMVKYQRLQEKYTTLLANAVMVNDVEAKTTFDLSQQN
                     ADMAYVMKSYFTIPDSTVSVTDQEVKSFYEKNKNAFRMNVPLAKITYFTKEIVPSDAD
                     FAEIEAQANEAYLKLVETANPASVVADYSDMPFRDVYLSANSLTEGQKSFVESASVSD
                     IKGPAREGDAFTIYKLLDKTVAPDSVHLRMMAIPDASMAGQDSIVTHFVDSIFNEIQS
                     GKSFAEVANSLNPQSNGGDVGWAREIDLAQAGIDVVKAAFSAPVGQAFKMTVPGQQII
                     LQVEEKTVPVQKYKIATINMPVVVSEKTSNNVDNELNQFVSDPNVSKNFNELATQKGY
                     FVMPDASVSANDFSLMQIPGSRQVVTWAVNEKKMGTVRKFDITNMRIIARVDQVYPAG
                     VAPLQEVATSIRSRLSNDKKAEKIISDLTAKNLTSLDAYATEMQSNVDTVKFVNFTTR
                     NITGLGFEPTLNAFSAYAPLNTLIPPAKGNMGVYVVNVLSRTQGTETYDAKAQKDLIQ
                     SNNAYMLQMQSLETLKKKLKVEDNRYVFF"
     gene            complement(5075..6337)
                     /locus_tag="PLI30_01775"
     CDS             complement(5075..6337)
                     /locus_tag="PLI30_01775"
                     /inference="COORDINATES: protein motif:HMM:NF013741.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HOI78312.1"
                     /translation="MSFTSLIIGWFILLFLSAFFSGMEVAFVSSDKLRFAMRQKDKGF
                     YNLMLNTIYSHPRQFLATLLLGNLMVQVVFMYLSLLVAFPYIQQHITQNTFVIFLLII
                     VGATLIILLSGEFLPRAVLVRKPNFWVRVLVVPAFFFYVLFFPVAKIFVSFSKAVLAV
                     FGVRSDRTTENALGRLELDSYVRKGFEEISDEKEVDSEVKIFRNALDFSSMRVRDCMV
                     PRADIVAINVDEDIEKLKDLFIESGISRILVYREDIDDIIGYIHVWEMFDVPQDWTKS
                     IATVSFVPESMQASKLMNDLMQQRKSIAVVVDEFGGTSGVVTMEDLVEEIFGDIEDEY
                     DNKARFVKQEGDKEFVLSGRVEIDTLNEDYGLDIPESESYSTVAGYLLHHTQRFPKTY
                     ETVIIGKYMFKILKVTARKIEVVRLTIN"
     gene            complement(6364..6990)
                     /gene="lptC"
                     /locus_tag="PLI30_01780"
     CDS             complement(6364..6990)
                     /gene="lptC"
                     /locus_tag="PLI30_01780"
                     /inference="COORDINATES: protein motif:HMM:TIGR04409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HOI78313.1"
                     /translation="MKERQQIHILKYITTAVFGVVMFLFIGSCGKKDEKLVHMKFDPE
                     TMPSMVTDSAVQLISDSGKTRYKIAADVWEVYDKAKEPFSYFPEGFYLERFDDRFNVE
                     ATVIADTAWHYNAKSLWRVKGNVEVKNMQGHEFKSDELFWDQKTAKIYSDKYIEIKRG
                     ALELKGYGFESNQQMTDYRIMRPHDGRLPFEEGPVATDSVQLQSDSIQ"
     gene            complement(7004..8365)
                     /locus_tag="PLI30_01785"
     CDS             complement(7004..8365)
                     /locus_tag="PLI30_01785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI78314.1"
                     /translation="MRKLLLSGFLAIATIAGLSAQKAGYDPVKAPFGHGEDSVKCRMN
                     LSLMTTSAKAENYKEALTPWNAVYENCPASSRNIYILGPRIFKSLYASETDAAKKKQY
                     LDKTMEIYDTRLKYYSDDKKGTVLAYKAYDYMELMGDKADSKIVYQWLGEALKEMKSE
                     MEPKDAYSYYMVASLTQFLTDPSKKNQYVSDYFTVAGYVDEAISKANAANDKANADYL
                     GLVKEGIVKAFVSSGAGDCKTLTDYYADKVEPNKADKAFLNEVINALGSVGCSESDLY
                     FTASEYLYKLEPSAGAAIGLANKSLRDKDYDTAIKYYHEGAQLETDKNKASDYMMQLA
                     GIFSNQRNFAKSRQAAYDALEFNPNNGEAYILIAQLYAASAQNIFPETEKRGLVFCAA
                     VDKLQKARSVDPSVSGKANGLINTYSGYFMDTETAFMMGIKAGESVFVPGWIGESTTV
                     RLK"
     gene            complement(8476..9819)
                     /locus_tag="PLI30_01790"
     CDS             complement(8476..9819)
                     /locus_tag="PLI30_01790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI78315.1"
                     /translation="MNITTKRHLSLLVFFTVTTAIFAQQVGSNSPYGRYGYGLLSNQS
                     LGASEAMGGISYGLRRSQQVNPGNPASYSKLDSLTFIFDLGVSGHYASYSDGLNKQNF
                     YNGNLDYIAIQFPVLSKVGASIGLLPYSKVGYNYGQTRSLSDIVYEEVFRGTGGLSQI
                     YAGIAYEPIPYFSVGANVSYLFGNFSYSSVSVPRTATGATIGEEKKKYSIRDLKYDFG
                     AQVTFPVDKTSSFTLGAVFSPKLASKSDVYATEMMFLSDPYTNPYQNPSEVLRDDTLS
                     SQQFHLPATYGLGVTYSNTNLLVGLDGTFQQWNGLDYPEVLDGLTPENRFNNAYRINA
                     GAEYVVDPYSRDFFSRVRLRAGISYGNSYANVSVYNPSTNQSVGVGGFKEYGINFGLG
                     LPFRDSMSGRLSLLNIGFGYTSQRPDLPYMIKQDMFKISLNMNINEFWFFKRQFN"
     gene            complement(9952..10497)
                     /locus_tag="PLI30_01795"
     CDS             complement(9952..10497)
                     /locus_tag="PLI30_01795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007766435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HOI78316.1"
                     /translation="MDFLEFVSTRQSDRAFDPDRPVEKEKIERILEAARLAPSACNAQ
                     PWHMVVVDDPELKNRVADATSARALGMNHFTKQAPVHILLVEERVNLTSGIGGWVKQK
                     DYAQMDLGIVAAHVVLAAHAEGLGSCIVGWFNEPKMRELLSIPDSKRVWLDIVVGYST
                     QAVRPKKRKTNGEIISYNKYK"
     gene            complement(10509..11114)
                     /locus_tag="PLI30_01800"
     CDS             complement(10509..11114)
                     /locus_tag="PLI30_01800"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019540981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HOI78317.1"
                     /translation="MFSGIVEEAATVVALEKDKGNLHITLRCSFTSELKIDQSIAHNG
                     VCLTVVSIEGDAYTVTAIQETLDRSNLGLLQVGSKVNLERSMKMDGRLDGHIVQGHVD
                     QTAVCTDVVEADGSWYYTLRYRFDKEMAKKGYLTVDKGSVTVNGVSLTVVTPTKDTFK
                     VAIIPFTYEMTNFHQIEKGTVVNLEFDIIGKYISRIVALQS"
     gene            complement(11167..12075)
                     /gene="dapA"
                     /locus_tag="PLI30_01805"
     CDS             complement(11167..12075)
                     /gene="dapA"
                     /locus_tag="PLI30_01805"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008148263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HOI78318.1"
                     /translation="MSKINLKGIGVALITPFKKDKSVDFDALGRLIDYQIDGGIDYLV
                     ALGTTAETPTLTRDERTEIVRFIVERNNNRLPLIVGVGGNNTAEIVHELKVSYFSNIA
                     GVLSVTPYYNKPSQEGLFQHYRALSEVSPRPIILYNVPGRTGVNMEAETTLRLAKSCP
                     NIVAIKEASGKMDQILQIIAGRPEGFSVISGDDGIAFDLIKSGGVGVISVLGNALPKE
                     FSEMIHQALAGNFKMAETENVRFTEAIRLIFKDGNPAGVKCMLSELGYIENELRLPLV
                     AVCDETKQQIIEELERLKVQVIDKKS"
     gene            12514..13374
                     /locus_tag="PLI30_01810"
     CDS             12514..13374
                     /locus_tag="PLI30_01810"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HOI78319.1"
                     /translation="MKHIKHCFYVALLPVFFSACNVVKSELKPTQIGICTNLANGEKM
                     QQYGYTYVEEGVSRFLAPTKPEEEFEIILNELANSPLPVKACNSFIPGNLKSVGNEAA
                     HHDILTFAETAFRRAQRAGVEIIVFGSGGSRSIPEGFPRKKGRAQFIELGKKMALIAQ
                     KFNIIIVLEPLNTTECNFINSVSEGGEIVKEINHRHFMLLADLYHMKMENESPDTLVK
                     YGKLIRHVHIAEKQDRAVPGTYDEDFRPYFNALKKMGYRGKISIEARWIDFDTQIPTA
                     METIKRQLNN"
     gene            13379..>13623
                     /locus_tag="PLI30_01815"
     CDS             13379..>13623
                     /locus_tag="PLI30_01815"
                     /inference="COORDINATES: protein motif:HMM:TIGR01409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocation signal
                     domain-containing protein"
                     /protein_id="HOI78320.1"
                     /translation="MESRRGFIKKSALGVAGIALGSTMDMSAKSYAGIIGANDRLNVA
                     IIGLGRRLSAFYEPIALKGANVQLLYLCDVMEKQRTRA"
BASE COUNT         3866 a         3180 c         2775 g         3802 t
ORIGIN      
        1 cctcaggacg ttacctgaac ctggccgacc tgatgaagtt gcccaacccg gtgggtgacc
       61 gcctccggca gacactgccg caaccgctgc gggtacccga gctggaagcc agcaattcga
      121 ttctacgggt actgcgccgc caggatgtgc tgctgcactt cccctaccag tcgttcaact
      181 acctgctgcg tttcctgctt caggccgcct tcgacccgaa ggtactggaa atcaaagtaa
      241 ctcaataccg tgtggcggaa aactcggctg tgatcaacag cctgatcagt gcggccaaga
      301 acggcaagaa ggtgaccgtg ttcgtggaac tgaaagcccg ttttgatgag gaaaacaact
      361 acctctcttc cgagttgatg caacaggcag gcgtaaagat aatctacagc ctgccggggc
      421 tgaaggtaca tgccaaactg gcttacgtgc ggaaaaggag taacgatccg gatgacccga
      481 tcaagggata tgcctacctg ggtaccggca acttcaacga gaaaacggcc cgcatctatt
      541 ccgacaaggg gttgctcacc tccaacaagg agattatccg tgatattgac gaagtgttcc
      601 gcgtgttgga gggaaagccc tacatgcatg atttcaggca tctgctggta acccagttca
      661 acatgctctc cgccatccat caaatgattg aacaggaaat cacagaagta gagagcgggc
      721 gcgaggggta tattgtccta aaaatgaaca gtctggaaga caaggggatg atcaatgccc
      781 tctatcgtgc cagcgaggca ggagtgaagg tcgacctgat tatccgcggc atctgctgcc
      841 tgatacccaa ccaaccctac agcaagaata tccgggtaac gcgtattgtg gatgcctacc
      901 tggaacatgc ccgtgtatgg tacttcctga acggtggtaa agaaacgatc tacctcacct
      961 ctgccgactg gatgcagcgc aaccttcacc gtcgtatcga agtggctttt ccggtttatt
     1021 ccgagccgtt gaagcggcag atcatcgata tcctgaagat ccaactggag gataaccaga
     1081 gcgctgtatg ggtgaacgag catctggata atgtctttaa aagggatacg gcatcaccgg
     1141 ataacacacc tatccgcgcg caacaggcaa tccacgacta cctgaaacag tctacaggac
     1201 aataagctaa gggtcgcgga aaatttccgt tttcagctaa tagctcgaag gaacatctgt
     1261 ggccagctcg ccatgttgtg taatatccag ccctctctct tcttgaattt cggttacccg
     1321 catcggaagg ataagatcag taagtttgta cagcaagaat gagcctccga aagtgaaagc
     1381 acctacgata agcagtgcca gcaaatgata caggaaaacg gtcggatcac cgtatataag
     1441 gccaacgttt tcggcaaata ctcccgtgag gatcatcccc gttatacctc cgataccgtg
     1501 gcatggaaaa acatccaacg tatcgtctat actgcttctc gatttcaacc gggtagcaaa
     1561 ataactcacc agcgacgctg tgcaaccgat aaaaatactc tgccccaccg ttacaaaacc
     1621 agcggaagga gtgatggcga ccaatccaac caccgcacct acagcagctc ccaccaccga
     1681 tctttttttt cctctggcgc catcagtaag catccaggta accatagccg ttgccgatgc
     1741 caaatttgtg ttcaaaaagg ccttgacggc aagttcattg gcagcaagcg ccgatcctgc
     1801 attaaaaccg aaccaaccga accaaagcaa cccggtaccc agtaagatat aaggaatatt
     1861 agtgggatta tcggttccac cgtcttttcg ttttttcagg aatatggcac cggaaagggc
     1921 tgcaaacccc gcagaaatgt gtacgactgt ccctccggca aagtcgagaa caccccactt
     1981 acgaagaaag ccttcgggat gccaggtcca atgagccaac ggcgcataaa taaaaatgga
     2041 gaaaaagact ataaagacca ttaacgacga aaaacgaatt ctttctgcaa cgccacccgt
     2101 gatgagtgcc ggggttataa tagcaaactt gagttgaaac accgcaaaca gggcaaatgg
     2161 aatggttgcc gaaaaagcag ggttgggctg caatcccaca ttgttgaaca tgaaaaaaga
     2221 tgcggggttt ccgataaagc ctcccaggct atcgccgaaa gcgaggctga agccaatcaa
     2281 aacccaaata acgctgatta ttcctaacgt gataaaactc tgaataagag tagacacaag
     2341 gttcttatac cgaaccattc ctccataaaa gaaagccagc ccgggtgtca ttaaaaatac
     2401 cagtgcggaa gctattagca tccaggcagt gtcagcattg tttatcgcca agcttgaaaa
     2461 ttctgtggat ttatccatcg gaattagcgc cgaaaccgct ataatgacaa taacaataaa
     2521 aggcaccgta taccttcttt tggagttttt ttcgttcata ctttgaaata tttctataac
     2581 ctaaattatc tatataatct taatatgcca caaatataat tattttgatt tattttacaa
     2641 tgctttaata gaataaatat attatttatc tatttattat atcatatagt catctcaaat
     2701 gtttcgaaaa aactcttaca agagacagct cttattgcct gggcacaaaa aaaagagagt
     2761 gcctcatatt ttttgagaca ccctctttct tgttttgtta tgaaacgaaa aacttagaag
     2821 aaaacgtaac ggttatcttc cactttcagt ttctttttta aggtctccaa agactgcatc
     2881 tgcaacatat atgcattgtt actttgaatc aggtctttct gagccttggc atcataggtt
     2941 tcagttccct gggttctgct caacacatta accacgtaga cgcccatatt ccctttggca
     3001 ggcggtatca gtgtattcaa cggtgcatag gcagagaaag cattcaaggt cggctcaaaa
     3061 cccaatcctg taatgttccg tgtagtgaag ttgacaaatt ttaccgtatc aacattcgac
     3121 tgcatttcag tagcataagc atccagactg gtcaggtttt tggcagtcag atcggaaatg
     3181 attttttcgg ctttcttatc gttgctcagt cgagaacgaa tgctcgttgc cacctcctgt
     3241 aagggggcca caccagccgg ataaacctga tctacacggg caattatacg catattggta
     3301 atgtcgaact tacgcacggt tcccattttt ttctcgttta ccgcccaggt aacgacctga
     3361 cgtgatcccg ggatctgcat caacgaaaaa tcgtttgctg aaacactcgc atccggcata
     3421 acaaaatatc ctttttgggt ggcaagttca ttgaagttct tgcttacgtt gggatcggaa
     3481 acaaactggt tgagttcgtt atctacgttg ttcgatgttt tttcactgac gacaacaggc
     3541 atattgatcg ttgcaatttt atatttctga acaggaacgg ttttttcttc cacctgaaga
     3601 ataatttgtt gcccgggaac tgtcatttta aaagcctgtc ccaccggtgc actgaatgcg
     3661 gctttcacca catcaattcc agcttgcgca agatcaatct cgcgagccca gcccacatca
     3721 ccaccattag actgtggatt caagctgttt gctacttctg caaatgattt tccgctttga
     3781 atctcgttaa aaatactgtc cacaaaatga gtaacgatac tgtcttgtcc cgccatagat
     3841 gcatcgggaa tagccatcat tctgagatga acggagtcgg gagctaccgt cttgtccaac
     3901 agtttataaa tagtaaatgc atcgccttcg cgagccgggc ctttgatgtc tgaaacagat
     3961 gcagactcca caaatgattt ctgaccttca gtcagtgaat tcgcagaaag atatacatct
     4021 ctgaacggca tgtcggaata atcggcaact acggaagccg gatttgcggt ttccaccaat
     4081 ttcaggtacg cttcattggc ttgcgcttcg atttcggcaa aatcagcatc actgggaacg
     4141 atctccttcg taaaataagt aattttggcc aatgggacat tcatccggaa agcgttttta
     4201 tttttttcgt aaaacgattt cacttcctga tcggtaaccg agaccgttga atcgggtatg
     4261 gtgaagtaac tcttcatcac ataggccata tcggcatttt gttgcgacaa gtcaaaagtg
     4321 gtttttgctt caacatcgtt caccatcacg gcattggcaa gcaacgtagt gtatttttcc
     4381 tgaagacgtt gatattttac catgttttcg atgaacaacc acatcgattt gtattgctca
     4441 actacagctc tctcttccgg ctgtagcgac tcaaccggag cgtttacagt ggataagaat
     4501 tgcatcaggc cttccctgtc gaacataccg gtttgcggat cgagaaagaa aggaagctgt
     4561 tgcagtaatg gcgatatcgt ttctccgtgt accagatcgt tgatttctgt ttttgaaact
     4621 gataagccaa gttttgccgc ttcgttgtcg agcatccgtt cacgaaccat ctgatcataa
     4681 accgcttccc ggatttgttg agatgtattt tcgtccaatg aagtctgtcc actgatcatt
     4741 ttttgaaaac tttcccattc ggtaatcctg tcagcgaatt cctgagtaga aatcacctgc
     4801 ccgtttacaa cgaaagcttt atcgcgcgat ttggcaaaaa gtgtgtttcc cgaggtgaat
     4861 aagtctccca aaataaatgc cagtaacgca atcccgataa tggcgaccaa aaacacgccc
     4921 ttatccctaa tcttctgtag tgtagccatt taactatcct gagtttttga taaacaatta
     4981 atattttaca ttcttttaaa caagagtgca aagttaataa aaattagaaa gataactcca
     5041 tatttttgaa aaaagtgatt cctgagtgaa agccttaatt aatagtcagc cgcaccactt
     5101 ctattttccg ggcggtaact ttcaatattt tgaacatata ctttccgatg atcactgttt
     5161 cgtaggtttt aggaaaccgc tgtgtgtgat gcaataaata cccggcaacg gtggaataac
     5221 tctccgattc gggaatatcg agcccataat cttcgtttaa ggtatcaatt tccaccctcc
     5281 ccgaaagcac aaactcttta tcaccttcct gtttcacaaa gcgggctttg ttgtcatatt
     5341 catcttctat atcaccgaaa atttcctcta ccagatcttc catagtcacc acacccgatg
     5401 ttcctccaaa ctcatccact acaacggcta tgctttttcg ctgctgcatg aggtcgttca
     5461 tcagtttgct ggcctgcata ctttcaggga cgaaagacac tgtggcaatg ctcttggtcc
     5521 agtcctgcgg gacatcgaac atttcccaaa cgtgaatgta tccgataatg tcgtcaatat
     5581 cttcccgata taccagaatt ctggaaatac cgctctcgat aaacaaatct ttcagctttt
     5641 caatatcttc gtccacattt atcgccacaa tatccgctct tggcaccatg caatcacgca
     5701 cgcgcataga agagaaatcg agggcgttcc tgaaaatttt cacttcggaa tccacttctt
     5761 tttcgtcaga aatctcttcg aacccttttc tcacatacga atccagttcc agccggccca
     5821 atgcattctc agttgtcctg tcggacctca cgccgaaaac agctaaaacg gctttggaaa
     5881 aagagacgaa aatttttgca acgggaaaga acagaacgta aaaaaagaaa gccggaacaa
     5941 ccaatacgcg tacccagaag ttgggtttac gtaccaaaac agcccgggga agaaattctc
     6001 cgctaagaag gataatgaga gtagctccaa cgattataag tagaaaaatc acaaaagtat
     6061 tttgtgtaat atgttgctga atgtaaggga aagctaccag caacgacagg tacataaaaa
     6121 ctacctgcac catgaggttt ccgagcagaa gcgtagccag gaactgacgc ggatgactat
     6181 atatggtatt caacatcagg ttgtaaaatc ctttgtcctt ttgtctcatc gcaaatctca
     6241 acttatccga tgacacaaac gccacttcca taccggagaa gaacgccgac aggaagagta
     6301 agataaacca tccgattatt agcgacgtga aagacattgt ttatggatta aggttgttga
     6361 atctcattgt atggaatcag attgtaactg caccgagtct gttgcgactg gtccttcttc
     6421 aaacggcaat ctgccgtcat gcggtctcat gatccggtaa tctgtcattt gctgattcga
     6481 ctcaaaaccg tatcccttca gttcaagcgc cccccgtttt atctcgatat atttatcgga
     6541 atagatctta gccgtttttt gatcccaaaa aagctcatca ctcttgaatt cgtgcccttg
     6601 catattcttt acctcaacat tccctttcac gcgccacagg ctttttgcgt tgtagtgcca
     6661 ggccgtgtca gcaataacgg tagcttcgac attaaaccga tcatcgaatc tttccaggta
     6721 aaagccttcg ggaaaataag agaacggttc tttggccttg tcatatactt cccacacatc
     6781 ggctgctatt ttataacggg ttttccccga gtcggagata agctgcacgg ccgaatccgt
     6841 aaccatggaa ggcatggttt ccggatcgaa tttcatatgg accagctttt catctttctt
     6901 tccacacgat ccgataaaaa gaaacataac aaccccaaaa actgcggttg ttatgtattt
     6961 taatatatgt atctgttgtc gttctttcaa caaatcgctg gttttatttc aaccttaccg
     7021 tggtagactc acctatccat ccggggacaa aaacggactc acctgccttg atacccatca
     7081 tgaaagccgt ttcggtatcc atgaaataac cggaataagt attgattaag ccatttgctt
     7141 ttccactaac gctaggatca acggatctgg ccttttgcaa cttgtcaact gccgcacaga
     7201 aaacgaggcc tctcttttcg gtttccggga aaatattttg agccgatgct gcatataact
     7261 gtgcgatcag gatatacgct tctccgttgt tgggattgaa ttccaatgca tcgtatgcgg
     7321 cctggcgcga tttggcgaag ttacgttgat tggaaaaaat acctgccaat tgcatcatgt
     7381 aatcggaagc tttatttttg tctgtttcca attgggcacc ttcgtggtag tacttgatgg
     7441 cagtatcgta atccttatct ctcaggctct tgttagccaa cccgatagct gcaccggcag
     7501 aaggttcgag tttgtaaagg tattccgacg cggtaaaata caagtcagat tcactgcaac
     7561 ctaccgatcc cagggcattg atcacttcgt tcagaaatgc tttgtctgcc ttgttgggtt
     7621 ccaccttatc ggcatagtaa tccgtgaggg tcttgcaatc gccggctcca ctgcttacaa
     7681 atgctttcac gataccttcc ttcaccaatc ccaggtaatc ggcatttgct ttatcgtttg
     7741 cggcatttgc tttgctgata gcttcgtcta cataaccggc aacggtaaaa tagtcgctta
     7801 catactgatt tttcttggac gggtcagtca gaaactgagt aagtgaggca accatataat
     7861 aggaataagc atctttaggt tccatctccg atttcatttc cttaagggct tcccctaacc
     7921 attgataaac aatcttcgaa tccgctttat cacccatcag ctccatatag tcataggcct
     7981 tgtatgccaa aacggtacct tttttgtcat ccgaataata tttcagtcgg gtatcgtata
     8041 tctccattgt tttatcgaga tattgtttct ttttggcagc atcggtctcc gaagcatata
     8101 aactcttgaa gatacgtggt cctaatatat atatatttct actggatgcg gggcagttct
     8161 cgtaaacagc attccagggc gttaatgctt ccttataatt ttcagcctta gcagaagtcg
     8221 tcataaggct caaattcata cggcatttta cgctgtcttc cccatgtccg aaaggagctt
     8281 ttaccggatc gtatcctgct ttctgggcgc tgagacctgc aatggttgct attgcaagaa
     8341 atcctgataa taataatttt ctcatattct tttttttaat ttttttttaa tcccgatttt
     8401 ggttgttaat aggacaatat gttaaataaa aagtttaatc tgttttgaat ttaaacgtca
     8461 caaagataca aaaaatcagt taaactgtct tttgaaaaac caaaattcat tgatattcat
     8521 gttcaaggaa attttaaaca tatcttgctt aatcatgtat ggcaagtcgg gacgctggga
     8581 tgtgtaacca aatccgatat taagcaatga caaccttccg gacatagaat ccctgaacgg
     8641 caaccccaat ccgaagttaa ttccatactc tttaaaacct ccaaccccta cactttgatt
     8701 ggtagatgga ttataaacgc tgacgttggc atatgaattt ccgtaggaaa taccggccct
     8761 taggcgtact ctactgaaaa aatcacggct gtacggatcg acaacgtatt ctgcgcctgc
     8821 attgatcctg taagcgttat tgaaccgatt ttcaggggtc aatccgtcta aaacctcggg
     8881 ataatccaat ccgttccatt gctgaaaagt tccgtctagt cctacaagca gatttgtatt
     8941 ggaataagtt actcccaatc cataggttgc cggcaaatga aactgctgac tggaaagagt
     9001 atcatcacgc agcacttcac tcggattttg atagggattg gtataagggt ctgacagaaa
     9061 catcatttcc gttgcataaa catcggattt tgaagctaat ttcggactga aaacagctcc
     9121 taacgtaaac gaacttgtct tgtcgacagg gaacgtgacc tgtgctccaa aatcatattt
     9181 taaatcccgg atggaatatt ttttcttttc ttcaccaata gtagcacccg tcgcagttct
     9241 cggcacagac acgctgctgt agctgaaatt cccaaaaaga taagaaacgt tagctccaac
     9301 ggagaaatac ggaatgggtt catacgcaat tccagcataa atctggctca accctccagt
     9361 tccacgaaat acttcctcgt aaacgatatc ggaaagcgaa cgtgtctgcc catagttata
     9421 tcctactttc gaatacggca aaagcccaat gctggcacca accttgctta ggacaggaaa
     9481 ctgtatggca atataatcga gattcccgtt gtaaaaattc tgcttgttca gtccgtcgct
     9541 gtaagaagca taatgccccg aaacaccaag gtcaaaaata aacgtaagtg aatcgagttt
     9601 ggagtatgat gccggatttc cgggattcac ctgctggctt cttctgagtc cgtagctgat
     9661 tccacccata gcttcggagg cacccaacga ttggttggac aaaaggccgt aaccgtacct
     9721 gccgtatggt gagttagacc ctacctgttg tgcaaaaata gcggtggtga cagtaaagaa
     9781 aacaagtaac gagagatgac gtttagttgt tatgttcatt ctttttcatt aaaatcaaca
     9841 agacgttggt cgattgtttc ataatttatt tttagggtac aaagatgatg tttttttgcg
     9901 atgcaaccaa caaaagaaca gaaagttcgt gttaaaaaga ttaaagagcg tttatttgta
     9961 tttattataa gaaatgattt cgccgtttgt tttgcgtttt ttgggacgaa cggcttgggt
    10021 actgtatccg acaacaatgt caagccatac tctcttagag tcggggattg acaacaattc
    10081 acgcatcttc ggctcattga accaaccgac aatgcaagac cccaaacctt cggcatgagc
    10141 tgccagcacg acatgagccg ctactattcc caggtccatt tgtgcatagt ccttttgttt
    10201 tacccagcct ccgatccccg atgtgaggtt cactctttcc tcaaccaaca aaatatggac
    10261 cggggcctgc ttggtaaaat ggttcatacc caatgcacgc gcagacgtgg catctgccac
    10321 tctgtttttc agttcaggat catccacgac aaccatgtgc cagggttgcg cattgcatgc
    10381 tgacggtgct aaccgagcag cttcgaggat acgttcaatt ttttcttttt cgaccggtct
    10441 atcgggatcg aatgctcggt cgctctgccg ggtggaaaca aattcaagaa aatccataca
    10501 ttagtcgttt aggactgcaa cgccacaatc ctgctgatat atttaccgat aatgtcgaat
    10561 tccaggttga cgactgtacc tttttctatc tgatgaaaat tggtcatttc gtatgtaaaa
    10621 ggaatgatcg ccactttaaa ggtatctttg gtgggtgtca ccacggttaa acttacacca
    10681 ttcaccgtaa ccgacccttt atctaccgtc aggtatccct tctttgccat ctccttatcg
    10741 aaacggtacc gtaaggtgta ataccaacta ccatctgctt caactacatc ggtacaaacg
    10801 gcagtttggt caacatgccc ctgaacgata tgcccgtcca acctgccgtc cattttcatg
    10861 ctacgttcaa gattcacctt gctacccacc tgcaacaatc caagattgga gcggtcgagc
    10921 gtctcctgaa tggcggttac ggtgtatgca tcaccctcta tggataccac tgtcagacaa
    10981 actccgttgt gcgcaatgct ctggtcgatt tttaattcgc tcgtgaacga gcaccttaac
    11041 gtgatgtgca ggttaccctt gtctttttcc aatgcaacca cggttgcagc ttcttcaaca
    11101 attccggaaa acataattat ttagttacga tttgtgattt acaaaatcag atgtagaagt
    11161 cagactttaa gactttttat ctattacctg aactttcaac ctctccaatt cttcaattat
    11221 ttgctgcttg gtttcatcac agaccgcaac caacggcagc ctgagttcat tttcgatgta
    11281 acccaattca ctgagcatgc attttactcc ggcaggattc ccatctttga aaatcaaccg
    11341 gatagcttct gtaaaacgca cattttccgt ttcagccatc ttgaaattac cggctaacgc
    11401 ctgatgtatc atttcagaaa actctttagg caaagcattt cccaaaacag atataactcc
    11461 tactccaccg gattttatca gatcaaatgc aattccatcg tcgccggaga taacggagaa
    11521 tccttcggga cggccagcaa taatttgcag gatctgatcc attttccccg atgcttcctt
    11581 gatggccaca atattcggac aagattttgc cagccgcaac gtagtttcgg cttccatatt
    11641 cacaccggtt cttccgggaa cattatacag aataatcggc cgtggtgaaa cctcactcaa
    11701 agcacgataa tgctgaaaaa gcccttcctg gctgggttta ttgtaatatg gagtaacaga
    11761 taatactcct gcaatgtttg agaaatatga taccttgagt tcatgaacga tttcagcggt
    11821 attgtttcct ccaactccta caataagggg gagacgattg ttgtttctct ccacgatgaa
    11881 tcgcacaatt tcggttcgtt catcgcgtgt cagtgttggg gtttcagcgg tagttcccag
    11941 tgcaacgagg taatcaatcc ccccatctat ctgataatct atcaaccggc ccaacgcatc
    12001 gaaatcgact gatttgtctt ttttaaacgg tgttatcagg gcaacaccta tcccttttaa
    12061 gttgattttt gacataaaaa caggtctttc atttttcctg agaaagcctc ccgacaacat
    12121 atggatactt cggatcgcaa agttacattt ttttcaacca tatccacata aattggacaa
    12181 ttacttttaa aaggaaatca ctttaatgga ttggtatttt tgttgaaatt cgaaatttaa
    12241 ctgtagctta acttgttttt tcattctgtt tacgtacaca ttagcatttt tattattttt
    12301 gtcggcgata tttccggaaa aacccttagc tcattaacat aatttaacgt cgatcaagtt
    12361 atttatttca tcctgtttac gtacacatta gtattttcat tatatttgtc cggatatttc
    12421 cgggaaaact cctgttcgtt ttctcaactt cgcaatacat tttggcaaac atatctttta
    12481 attctgcaga gacaaacacg taaaatgaaa cacatgaaac acataaaaca ctgtttttat
    12541 gttgcccttc ttccggtatt cttttctgca tgcaacgtag taaaaagtga gttgaagcct
    12601 actcagatag gaatctgtac aaatcttgcc aacggagaaa aaatgcagca atacggatac
    12661 acatatgtgg aagaaggtgt ctccagattt cttgcaccga caaagccgga ggaagagttc
    12721 gagataatat tgaatgaatt ggccaactcc cctttgcctg ttaaagcctg taacagcttt
    12781 atccccggaa acctgaaaag cgttggtaat gaagctgctc accacgatat tttaacgttt
    12841 gcagaaaccg ctttccgcag ggcacaacga gccggagtgg aaataattgt ctttggaagt
    12901 gggggttcac gttccatccc cgaaggattt ccacgcaaaa aaggccgggc acaattcatc
    12961 gaattaggca aaaaaatggc gttaattgct caaaaattca acataatcat cgtattggaa
    13021 ccccttaaca ctacagaatg caactttatc aattccgtat cggaaggagg agaaatcgtt
    13081 aaagagatca atcatcgtca cttcatgctg ttggcagatc tgtatcatat gaaaatggaa
    13141 aacgaaagtc cggataccct tgtaaagtat ggaaagctga tcaggcatgt tcatattgcg
    13201 gaaaaacagg acagggctgt accgggtaca tacgatgagg actttcgccc ttacttcaat
    13261 gcccttaaaa aaatgggtta tagaggaaaa atatccattg aagctcgctg gatagatttt
    13321 gacacccaga taccgacagc aatggaaacg ataaaaagac aactcaataa ttaaaaatat
    13381 ggaatcaaga agaggattta ttaaaaaatc ggcactcgga gtggcaggaa tagcactggg
    13441 aagcacaatg gacatgtcgg caaaaagtta tgccggcatt atcggagcga acgacaggtt
    13501 aaacgtagcc atcattgggc tgggacgcag gttaagtgcg ttttacgaac caatcgcctt
    13561 gaagggagct aatgtacagt tactttacct ctgtgatgtg atggagaagc aacgaacccg
    13621 ggc
//
LOCUS       DAOSDR010000024        13348 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrimonas sp. isolate AMR_MDS_1826
            NODE_1806_length_13348_cov_3.668698, whole genome shotgun sequence.
ACCESSION   DAOSDR010000024 DAOSDR010000000
VERSION     DAOSDR010000024.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863355
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrimonas sp. (wastewater metagenome)
  ORGANISM  Petrimonas sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Dysgonomonadaceae; Petrimonas.
REFERENCE   1  (bases 1 to 13348)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13348)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:10:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,720
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 35
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13348
                     /organism="Petrimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1806_length_13348_cov_3.668698"
                     /isolate="AMR_MDS_1826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2023866"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..679
                     /locus_tag="PLI30_01820"
     CDS             <1..679
                     /locus_tag="PLI30_01820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007655053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HOI78321.1"
                     /translation="PDPDKATGRAVIALPGGGYQGLAVFHEGFDWAPFFLERGIALVV
                     LKYRMPQGNPSVPFIDVKAAFDLVKQHASEWRINPEDIGIMGSSAGGHLASTYATHTS
                     GADRPAYQILLYPVITMDKTFTHAGSRKNLIGEDPSPELTSKYSNDKQVTGETPPAFV
                     IFAADDKVVPPQNGFRYVEALQRNNVPVTFLLYPAGGHGFGYRESYSYHSLFLSELSK
                     WLEGLRE"
     gene            745..3519
                     /gene="acnA"
                     /locus_tag="PLI30_01825"
     CDS             745..3519
                     /gene="acnA"
                     /locus_tag="PLI30_01825"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: protein motif:HMM:NF009520.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003994 - aconitate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase AcnA"
                     /protein_id="HOI78322.1"
                     /translation="MAYSTARAVETIEVNGHSYRYSSLKNLPGEAIEHLPFSIRILLE
                     NVLRNYDGFSITDDHIQTLTHWSPNPVDKDIPFKPARILMQDFTGVPAVVDMASLRAE
                     YVRHGKNGQKINPAIPVDLVIDHSVQVDYFGTNYSYSKNVELEYERNKERYELLKWAQ
                     KGLKNFTVVPPGMGICHQVNLEYLAQGITIRDGWLFPDTLVGTDSHTPMVNGIGVVGW
                     GVGGIEAEAAMLGQPIFFTCPEVIGLKLAGTIPAGCTATDMVLSITKVLREKGVVGKF
                     VEVFGDGLDNLTVTDRATIANMSPEFGCTVTYFPIDNRTLEYMHVTNRSPEQIKIVEE
                     YSKENLLWRTGNEKIAYSSVVEFDLSTLEPTVSGPKRPQDKIPVKELGYKFSELLEKE
                     HNRKYQSPLQRSESAWLADGGSGTEFTFGKTPVEGAAPHEIIAESLQSVRIKHKNKEL
                     ILSDGSIVIAAITSCTNTSNPAVMIGAGLLARNAVEKGLRTKSWVKTSLAPGSKVVTQ
                     YLKRAGLNEYFDALRFHTVGYGCTSCIGNSGPLPPQIAEAVEKGDLVVASVLSGNRNF
                     EARVHPQVKMNFLMSPMLVVAYALAGRVDINLMEEPLDYDPNGNPVYLADVWPGREEI
                     VETINKCVKQNDFQEVYDVIFEGSKDWQELKVNLDENFEWNRQSTYIKEAPFFKDLTV
                     DPDPMINIRNARVLLYLGDSVTTDHISPAGSFREESAAGQYLKAHGVGKADFNSYGAR
                     RGNHEVMMRGTFANVRIRNRIVDREGGFSRYFPTDEVKTVYETAMEYKRDKTPLIILA
                     GKEYGSGSSRDWAAKGTFLLGVKAVIAESFERIHRSNLVGMGVAPLVFLAGENAETLG
                     LDGTETYNITGLADNLIPHKLLEVEAVHPDGKVTVFEVEVRLDSAIEIEYYRNNGILQ
                     YVLRDYLKNS"
     gene            complement(3541..4287)
                     /locus_tag="PLI30_01830"
     CDS             complement(3541..4287)
                     /locus_tag="PLI30_01830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006842476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="creatininase family protein"
                     /protein_id="HOI78323.1"
                     /translation="MNSIDLRSANWKDVKEASYDAVVLPWGAFEPHNYHLPYLTDCYL
                     SHHIALESALLAYEKSGVLCAVLPPVYFGSQNPGQWDLPLCIHTNSETQKAILCDIVD
                     SLHGQGLKKLVIVNGHGGNTFKTYIRDLAKKYPDFTVIAVDWWSIVPTGAYFEEKIDE
                     HGGEQETSVLLHYRPDLVKMEQAGNGKTSPLPMESINQKVGWLPRPWQQVSEDTGIGN
                     PAKSTAEKGKRYAEAVVGKIAGLLVELKAW"
     gene            complement(4306..5469)
                     /locus_tag="PLI30_01835"
     CDS             complement(4306..5469)
                     /locus_tag="PLI30_01835"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOI78324.1"
                     /translation="MNNKTIAFIANFTGIFLFGISMVIIGSILPLLKARFGMSDIAAG
                     GLFSVLPVGMLAGSVLFGPVVDKFGYRWVLSVASLFLSLGFFGIVHVGSLHLLRAGIF
                     FFGLGGGVINGGTSALVSDLSEGKNKIINLNWLGLFYGIGAFSMPMILSRIDEIHYTL
                     VIDVVSGISLAVALIFLLINYPLTVQKEKISLKLIPVFVKNKLFMAICFYLFFQSAFE
                     AIINNWSVSFFIDKLAVPQYQALIALSVSVSGLVCMRILTGSVLKKWHHFRLIQLSLL
                     LFSLGLICLVLPASYFMHLLGMFLIGSGLAPGFPVMLGLVGEIFKEVSGTAFSFAMLI
                     ALTGNTIINYSIGILTEKFGMNVFPYVVLVEIVMMIFIFLLIGKADKKTAIRY"
     gene            complement(5487..6365)
                     /locus_tag="PLI30_01840"
     CDS             complement(5487..6365)
                     /locus_tag="PLI30_01840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006842794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HOI78325.1"
                     /translation="MADIKKNAVLSELLTEHSELIPVVNRFGIKLGVGEKTIEKICLE
                     NDLNIDFILAILNVYLDEEYNPEKDLDAFDVSLIVDYLKRTVENYMQASVPNLEKHFT
                     PFIAMSGGENEELKLLHKIFYQFKSELSEHLQQGLEQLSDYPNELLHDLKNIIIRHIS
                     GNFNQNLAYAVIFSITALEKELHIHNRLLEKVLRPKLKELDSGHIEHLNHTFSDEHKL
                     QEENKQLTNREIEVLKLIVQGRLNKEIADKLNISLNTVLSHRKNIIAKTGIKTVSGLT
                     FYSISNGYVSPGDHVV"
     gene            complement(6366..6704)
                     /locus_tag="PLI30_01845"
     CDS             complement(6366..6704)
                     /locus_tag="PLI30_01845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019539739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide-stimulated promoter family
                     protein"
                     /protein_id="HOI78326.1"
                     /translation="MNEGEKKVVNKMMAIYCRANHKHVTGLCEECTVLKNYAMKRLEN
                     CPFGEKKPTCGSCTVHCYKNDMRLKIKEVMRFSGPRMLFWHPIDAVRHFYKEYRRDRF
                     SSVAEKSFRK"
     gene            complement(6856..7545)
                     /gene="mtgA"
                     /locus_tag="PLI30_01850"
     CDS             complement(6856..7545)
                     /gene="mtgA"
                     /locus_tag="PLI30_01850"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007663454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monofunctional biosynthetic peptidoglycan
                     transglycosylase"
                     /protein_id="HOI78327.1"
                     /translation="MKKIPRIFGKIVLFFFLWSVVSVIGYRFVPVYFTPLMGIRMTEQ
                     IAEGRKPEVKHKWVSYTRISNNMKRAVVASEDQRFFNHNGFDKVEIKKALKENKTRKR
                     PRGASTISQQTAKNVFLWPRSSWLRKGLEAYFTVLIEFFWTKERILTVYLNCMEVGDG
                     IYGVEAVAHEHFNTTAEKLSASQSALVAATLPNPLKFSSKKPSSYIRQRQSHILRQMR
                     TVQLPPASEKK"
     gene            complement(7553..8008)
                     /locus_tag="PLI30_01855"
     CDS             complement(7553..8008)
                     /locus_tag="PLI30_01855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI78328.1"
                     /translation="MELELNGHNKQEYPPMHTTEHILNQTMVRMFGCARSKNTHIERK
                     KSKADYFLSQSPTPEQVQTIEAGVNEIIGRHLPVSEEFLPVSAAAEIVDLSKLPDNVS
                     ETLRIVRVGDYDVCACIGSHVGNTSEIGRFEIISHDFSDGRWRVRFKLW"
     gene            complement(8040..9344)
                     /locus_tag="PLI30_01860"
     CDS             complement(8040..9344)
                     /locus_tag="PLI30_01860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI78329.1"
                     /translation="MKGIVCAVLISFFIPVAGAQTPMINRDSIPHYFDQVKQATRENV
                     KIWDKDIYGPIMLIEEGTRVIYANEPDKDNVLVARRGYYTGVLPKEISFQNTSLDWNG
                     KTWATVVLPLSPNKYQRLDLITHELFHSAQLSLGFDIRGSDNYHLDEKDGRVFLRLEL
                     EALKKALRARRLSLAEEHLRNALIFRKYRHLVYSGSQLSENKLELLEGLATYTGQMMS
                     GRDKWQLREYLIARLEDYPNTPSFVRSFAYETVAVYGFFLYQKNNNWNKGISGETDLT
                     EFFEEAFELDMRIVLPSYVRQLSEDYRGKEIRDEETLRSEKHTLTLNELRDKFLEKPR
                     LEIKLEDMNMSFDPVNPIPLDVDEGTVYPTIRISDNWGILTVTGGGALLSPGLVWVVV
                     SEPVEIGEDEITGEGWRIELNKGYYLEKNNQGNYLMTKKKNE"
     gene            complement(9514..10242)
                     /locus_tag="PLI30_01865"
     CDS             complement(9514..10242)
                     /locus_tag="PLI30_01865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014225579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA threonylcarbamoyladenosine dehydratase"
                     /protein_id="HOI78330.1"
                     /translation="MGSRYLDLAARKRVIIFGVGGVGSWCAEMLIRSGITHLTLVDSD
                     RVAVTNINRQLMATVKTIGQVKVEVLKERLLEINPNAEVVSMQVVYSPETAGSFKLES
                     YDFIIDAIDSLSNKVHLIRLASQMPGVFFSSMGAALKIDPSYIRVDEFWKVKGCPLAA
                     ALRTRIKKLGGLEKKFLCVYSNEARENKGEKPFLEEQITPVSQWDTKKARINGTMSYL
                     PAIFGMTLASLVVKDIDIHSGGES"
     gene            complement(10338..11117)
                     /locus_tag="PLI30_01870"
     CDS             complement(10338..11117)
                     /locus_tag="PLI30_01870"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019539741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOI78331.1"
                     /translation="MNHTALITGASRGLGAEFARLFASKGTNLILVARSEKELNDLKD
                     ELENTFNISVRTITKDLTKPENVRFVYDEIKDAGIEVDYLVNNAGFGDFGYFADTEWV
                     RHRQMIDLNISALSNLCHLFVQDWIPRKSGKILNIASTAAFQPGPGMSVYFASKAYVA
                     SFSQAIAYEFQKYGITVTALCPGPVRTNFGIAARMQHPNGFLKDMKTPLPGEVVAFGY
                     HAMMKGKTLVIQGGLNKFVANLVRFIPRKWLITFSAKVISW"
     gene            complement(11293..12750)
                     /gene="rodA"
                     /locus_tag="PLI30_01875"
     CDS             complement(11293..12750)
                     /gene="rodA"
                     /locus_tag="PLI30_01875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019539742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein RodA"
                     /protein_id="HOI78332.1"
                     /translation="MYRGTREIQEKGRVDRFTALMYLLFIVLGWLNIYAASFDLENAV
                     SMFDLSGRAGMQLVWMGTSLLLAFALLKIDTSFYETYAFVAYIAGIALLVITLVVAPE
                     IKGSRSWLVIGPVRLQPAEFMKFIIALTLAKIFNVYNFRLMERKNLIIVFSLIFLPLV
                     LVILQSETGTALVYLSLIFVLYREGLPGGILFMGVAAILYFVLAIRFSDVQLGMLSVG
                     EFIAVILIIVFAAAMVWNYVKRRETAFIIIGLGLIVLAAGWIMHFFNLPVNPGLLALV
                     ALGLVAVYLAYLSLRFWKKVYLYILLFVVGSFAFVESSEYVFNDVLQPHQQMRIKVTL
                     GMEQDLRGSGYHVGQSKIAIGSGGMSGKGFLNGTQTKLKYVPEQDTDFIFCTIGEEWG
                     FIGSTIILLLFAVFILRLISLAERQTTIFGRVYGYGVVSIFLFHLLVNIGMVIGIMPV
                     IGIPLPFFSYGGSSLWGFTILLFIFLRIDKGRKRA"
     gene            complement(12734..>13348)
                     /locus_tag="PLI30_01880"
     CDS             complement(12734..>13348)
                     /locus_tag="PLI30_01880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019539743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HOI78333.1"
                     /translation="AFKNDRWVAHNIISIAIGQGEILATPLQLANYTALIANRGYYHK
                     PHVVKARKGAPLDTLYSKRRESNIRREHFELTVAGMADAVTHGTCRRANMEPFLTVCG
                     KTGTAENPHGEDHSLFIGFAPKDDPKVAIAVIVENGGFGATNAVPIGRLMMQKYLMGE
                     IMPQDQVLEKTIASRVILPFAYRRNASAQRIDTTATQVRNVQRN"
BASE COUNT         3744 a         3198 c         2870 g         3536 t
ORIGIN      
        1 gcccgatccc gataaagcta caggaagagc agttattgcc ttacccggcg gaggatatca
       61 ggggctggct gtttttcacg aagggttcga ttgggcacct tttttcctgg aaaggggcat
      121 tgctcttgtc gtactgaagt accggatgcc gcaaggcaat ccctccgttc cgtttattga
      181 tgttaaggca gctttcgatc tggtgaaaca gcatgcttcg gaatggcgca ttaatccgga
      241 ggatatcggt ataatgggct catcggcagg cgggcacctg gcttcaactt acgctacgca
      301 tacgagcggc gccgaccgtc ccgcctatca gatactgctt tacccggtaa taaccatgga
      361 taaaacattt acacacgctg gatcgagaaa aaacctgatc ggggaagatc cttcgccaga
      421 acttaccagc aaatactcta acgataaaca agtaaccgga gagacgcctc cggcgtttgt
      481 tatcttcgct gcagacgata aggttgtccc tccgcaaaac ggattccgat atgttgaagc
      541 attgcaacgg aacaacgtcc cggtaacatt tttgttgtat ccggccgggg gtcacggttt
      601 cggatacagg gaatcctatt cgtatcattc tctttttctt tccgaactat cgaagtggct
      661 ggaggggtta cgtgaataga ccttcaaaag acaggtaagc ggaaaatatg tttaactttt
      721 gtgttaattt taacaattaa aattatggct tattcaactg caagagctgt cgaaaccatt
      781 gaagttaatg gccacagcta tcgttacagt tcattgaaga atctgccggg ggaggccatt
      841 gagcacttac ccttcagtat ccggattctt ctggaaaacg tgttgagaaa ttacgacggc
      901 ttcagcatca ccgatgatca tattcagacg ttgacccatt ggtcgcccaa tcccgtggac
      961 aaagatatcc cgtttaaacc ggcacgtatc ttgatgcagg attttaccgg cgtgccggct
     1021 gtagtggata tggcttcact aagagctgaa tacgtccgtc acggaaaaaa cggacaaaaa
     1081 atcaatccgg ctattccggt cgatttggtt atcgatcatt cggttcaagt ggattatttc
     1141 gggaccaatt actcctacag caagaacgtg gagttggaat acgaaaggaa caaggagcgt
     1201 tacgaactgc tgaaatgggc acaaaaagga ttgaaaaact ttaccgttgt gccacctgga
     1261 atgggtatat gccatcaggt aaacctggaa tacctggctc agggaatcac aatacgcgac
     1321 ggatggttgt ttcccgacac cctggtggga accgactcac acacaccaat ggtaaacggg
     1381 atcggagttg tcggctgggg cgtaggtgga attgaagcgg aagctgccat gctgggacag
     1441 cctattttct tcacttgtcc cgaagttatc gggctaaaac ttgccggcac cattccggcg
     1501 ggctgtacgg ccacagatat ggtactgtcc attacaaaag tgctgcgtga aaaaggtgtg
     1561 gtgggaaaat tcgtggaagt ttttggtgac gggctggaca acctgacggt aaccgaccgg
     1621 gctaccattg ccaatatgtc acccgaattt ggatgtacgg taacctattt tcccattgac
     1681 aaccggacgt tggaatacat gcacgttacc aaccgttcac cggagcagat aaagattgtg
     1741 gaggaataca gcaaggaaaa cctgttgtgg agaacaggca acgagaagat cgcttattca
     1801 tcggtagtgg agttcgacct atccacgctg gagccgaccg tctccggccc aaaacggcct
     1861 caggacaaaa ttccggtaaa agagctggga tacaaatttt ccgagttgct tgaaaaagaa
     1921 cacaaccgga agtaccaatc ccctctgcaa cgttcggagt cagcatggtt ggccgacggt
     1981 ggttcgggaa cagagttcac cttcggtaaa acgcctgtcg aaggcgctgc tccacacgaa
     2041 atcattgcgg aatccctgca atccgtacgc attaaacaca agaacaaaga acttatcctc
     2101 agcgacggaa gcatcgttat tgcggccatc accagctgta ccaacacctc taatcctgcc
     2161 gtgatgattg gcgccgggtt attggcccgt aatgcggtag aaaaaggatt aaggaccaag
     2221 tcgtgggtaa aaacttcgct ggcccccggc tcaaaagtgg ttacgcagta cttgaaacgg
     2281 gcgggactaa atgaatattt tgatgcgttg cgtttccata ccgtaggtta tggatgcacc
     2341 tcctgtatcg gtaactccgg tcctttaccg ccacaaatag cggaagccgt tgagaaagga
     2401 gatctggtag tggcgtcagt cttatcgggg aaccgaaatt tcgaggcacg ggtccacccg
     2461 caggtcaaaa tgaatttcct gatgtcgccc atgctggtag tagcttatgc attggccgga
     2521 cgcgtcgata ttaaccttat ggaggaaccc ctggattatg atcctaacgg aaatcctgtc
     2581 tatctggcag acgtttggcc cggtcgggaa gagattgtgg aaaccatcaa caaatgtgtg
     2641 aaacaaaacg attttcaaga agtgtatgat gttattttcg aaggttcaaa agattggcag
     2701 gagctgaaag taaatctcga tgaaaatttc gaatggaaca ggcaatccac ctatataaaa
     2761 gaagctccgt tttttaaaga cctcactgta gaccccgacc cgatgatcaa tatccgaaac
     2821 gcccgcgtac ttctctacct gggcgactcg gtaaccaccg atcacatttc gccggcaggt
     2881 tcattccggg aagagagtgc cgcaggacag tacctgaaag cccacggagt ggggaaagcg
     2941 gattttaact cgtacggggc acggcgcgga aaccacgaag tgatgatgcg aggtactttt
     3001 gccaacgtgc gtatcagaaa cagaatcgtt gaccgggaag gtggtttcag ccggtatttt
     3061 ccgaccgatg aggtaaaaac cgtttacgaa acagcgatgg agtataaacg ggataaaacg
     3121 cccttaatca tcttagcggg taaagaatac ggttcaggtt cttcccgtga ctgggcagcc
     3181 aaaggaactt tcctgttggg tgtaaaagcc gtcatagccg aaagcttcga acgcattcac
     3241 cggagtaatc tcgtaggaat gggggtagca ccgcttgtat tcctcgcagg tgagaatgcg
     3301 gaaaccctcg gtctggacgg aaccgaaacc tacaacatca ccgggctggc cgacaacctg
     3361 atcccccata aattactgga ggttgaagcg gtacatcctg acggaaaagt gaccgtattc
     3421 gaagtggaag tacgtttgga ttccgctatc gaaattgaat actacagaaa caacggaatc
     3481 ttgcagtacg tgctgaggga ttacctgaag aacagttgat aaaaatgttg tggataagat
     3541 ttaccacgct ttcaactcaa ccagtaaacc ggcaattttc cccaccacag cttcggcata
     3601 tcttttgcct ttttcggcgg tggattttgc aggattacct atccctgtgt cttcggaaac
     3661 ctgttgccag ggtcgtggta accagccaac tttttgattg atggactcca tcggtaaggg
     3721 agatgttttg ccattaccgg cttgttccat cttcaccaaa tcgggacggt aatggagcag
     3781 caccgaggtt tcctgctccc caccgtgctc atcgatcttc tcttcaaagt aggcaccggt
     3841 aggcacaatg gaccaccagt ccacggcgat aacggtaaaa tcgggatatt tcttggctaa
     3901 atcgcgaatg taggttttga atgtattgcc gccgtgtccg tttacgataa ccagcttttt
     3961 taatccttgc ccgtgcaggg aatcgactat atcgcacaat atggcttttt gcgtctcgct
     4021 gttcgtgtga atgcacaacg gcaagtccca ttgtccgggg ttttgtgatc cgaaataaac
     4081 cggcggcaga acagcacaca acacgccgga tttctcataa gcgagtaaag cgctctccaa
     4141 ggctatatga tgcgataagt aacaatccgt aaggtacggt aaatgataat tgtgcggctc
     4201 aaaagcgccc cagggtaata ccacggcatc gtagctggct tcttttacgt ccttccagtt
     4261 ggcagacctt aaatcaattg agttcatatg ttgaaaaatt aaatgtcaat aacgaatagc
     4321 cgttttctta tctgcttttc caatcaacag gaaaataaaa atcatcatta caatttccac
     4381 aagaaccaca tacggaaaaa cattcattcc aaacttttcg gtcagtatgc cgattgaata
     4441 atttataatt gtatttccgg tgagcgcaat gagcatcgca aaactgaaag cagtcccgga
     4501 aacctccttg aagatttcac ctaccaaacc cagcatgacc ggaaaacccg gggccaggcc
     4561 gctaccgata agaaacattc ccagcaagtg cataaagtag gatgccggta gcaccagaca
     4621 gatcaacccg agcgaaaaca gcaataacga caattgaatc aaccgaaaat gatgccattt
     4681 tttcaatacg cttcccgtaa gaattctcat gcaaaccagt cctgagacag aaaccgataa
     4741 tgctatcaat gcctgatatt gaggcacggc aagtttatca ataaaaaacg aaaccgacca
     4801 attgttgatt atggcttcga aggccgactg aaaaaacagg taaaaacaaa tggccataaa
     4861 cagcttgttc tttacaaaaa caggaatgag ctttaacgat attttttcct tttgaacggt
     4921 caacggataa ttgatgagaa gaaaaattaa ggcaacagcc aggctgatgc cactcaccac
     4981 atcaataacc aaggtgtaat gtatctcatc tatccggctc aggatcattg gcatggaaaa
     5041 tgcaccaata ccgtaaaaca atcctagcca gttcaggttt atgattttat tttttccttc
     5101 gcttaagtcg gaaaccaatg cgctggtacc gccgttaatg actccaccgc ccaatccgaa
     5161 aaagaaaata ccggccctca acaagtgaag ggaacccacg tggactatcc cgaaaaaccc
     5221 gagcgacaga aacagcgaag caacggaaag cacccacctg taaccgaact tgtctacaac
     5281 aggcccaaac aaaaccgagc cggcaagcat accaacggga agaacggaga ataaacctcc
     5341 ggcagcaata tcagacattc cgaaacgggc cttcagcaac ggcaaaatcg aaccgataat
     5401 caccatggaa attccgaaaa gaaagatgcc cgtaaagttg gctataaatg caattgtttt
     5461 attattcata ttcctttttg cctttgctaa acaacatggt cgccgggaga aacatatccg
     5521 ttggaaatag agtaaaacgt aagcccggaa accgttttaa taccggtctt cgcaataata
     5581 tttttacggt gcgaaaggac tgtgttcagg cttatgttca gcttatcggc aatctctttg
     5641 tttaaacgtc cctgaactat aagcttcagt acctctattt cccggttggt caactgctta
     5701 ttctcctctt gcaatttatg ttcatcggaa aaggtatggt tcaggtgttc gatatgtccg
     5761 gaatccagtt ccttgagttt cggacgcaac actttttcca gcaaccggtt atgaatgtgc
     5821 agctcctttt cgagggcagt tattgaaaaa ataaccgcgt aagcaaggtt ttggttaaaa
     5881 tttcccgaaa tatgcctgat gataatgttt tttaaatcgt gcaacaattc atttgggtag
     5941 tcactgagct gctcaaggcc ttgctgcaga tgttcagaaa gctccgattt gaactgatag
     6001 aatattttat gcaacagttt tagttcttca ttttcgccac cactcatcgc gataaaaggg
     6061 gtgaagtgct tttccaggtt cggaacagac gcctgcatat aattttcgac ggtacgttta
     6121 agataatcca cgataagtga gacatcaaac gcatcgagat ccttttccgg attatattct
     6181 tcatccaggt aaacattcag gatggccaga atgaaatcaa tgttcaggtc attttccaag
     6241 cagatttttt cgatcgtttt ctctcccact cccagtttta tgccaaacct gttcacaaca
     6301 ggtataagtt cactgtgctc ggtaagcaat tcagataaga ccgcattttt tttaatgtct
     6361 gccatttatt tccggaaaga tttttcagca accgaagaga agcgatcccg cctgtattct
     6421 ttgtagaaat gccggacagc atctatggga tgccaaaaca gcattctagg gcctgaaaat
     6481 cgcattactt cttttatttt caatcgcata tcgtttttat agcaatgaac agtgcaggat
     6541 ccgcaggtag gttttttttc accgaaaggg caattttcaa gccgtttcat tgcataattt
     6601 ttcagtacgg tacattcttc acacagtccc gtcacatgtt tatgattcgc cctacaatag
     6661 atggccatca tcttgttcac cacttttttt tcaccttcgt tcatatatcg tttaaacagc
     6721 caggcgcgtt ctgtaatcag cgttattggt ataagcttga ttattgcctg agtccgtttc
     6781 tataaagaac cgtaaaagta acccttttat tttttttctc aaaatcggga caaaatgatt
     6841 tgatgaaaag aagagtcact tcttttccga agccggagga agttgtactg tacgcatttg
     6901 ccggaggata tgcgattgcc gttgcctgat atatgaagac ggtttttttg agctaaattt
     6961 cagcggatta ggcaatgttg cggccaccaa tgccgactgt gaagccgaaa gtttctccgc
     7021 cgtagtattg aaatgttcat gggcaaccgc ttccacgcca taaatcccgt cacccacctc
     7081 catacagttc aggtagaccg tcaaaatacg ttcctttgtc cagaaaaatt caattaaaac
     7141 tgtgaaatag gcttccaatc cttttctcag ccacgacgac cggggccaga gaaacacgtt
     7201 tttagccgtt tgctgactga tggtgcttgc tccccgggga cgtttacggg ttttattctc
     7261 tttgagggct tttttaattt ccaccttgtc gaagccgttg tgattaaaga aacgctggtc
     7321 ttccgaggcg acaacagcac gtttcatatt attcgaaata cgtgtgtacg aaacccattt
     7381 gtgcttaacc tcaggctttc tcccctctgc gatctgttcc gtcattctta ttcccatcag
     7441 tggagtaaaa tagacaggga caaacctgta ccctatcacg gatactacgc tccagagaaa
     7501 gaaaaaaagc actattttac cgaagatacg aggtattttt ttcatgactt ctctaccaca
     7561 atttgaacct tacccgccac cttccatcgc taaaatcgtg gctaataatt tcaaaacgcc
     7621 cgatttccga tgtgtttcca acatgtgaac cgatgcaggc gcaaacatca tagtcaccta
     7681 ccctgacaat acgcaatgtt tctgatacat tatcgggaag ttttgataaa tccactatct
     7741 ctgctgccgc tgagaccggt agaaattctt ctgaaacggg cagatgacgg ccgataatct
     7801 cgtttacgcc cgcctcaatg gtttgtactt gttcaggggt gggcgattga gacaaaaaat
     7861 aatcggcttt gcttttcttc cgttctatgt gtgtattttt cgagcgggca caaccgaaca
     7921 tccttaccat tgtctggtta agaatatgtt ccgtagtgtg catgggaggg tattcctgtt
     7981 tgttgtgtcc gttaagctca agttccataa tccgtttcaa agcacaaaga taagaaaaat
     8041 cactcgtttt ttttcttcgt catcaaataa ttcccctgat tgtttttttc aaggtaatat
     8101 cctttgttca gctcaatgcg ccacccttcg ccggtaattt catcttcgcc gatctccacc
     8161 ggttcagaaa caaccaccca tactagtccg ggcgacaaca gcgcacctcc gccggtcacc
     8221 gtcaagattc cccagttgtc gcttatgcgg atggtgggat aaaccgtccc ttcgtcaaca
     8281 tccaggggaa tcgggttaac cgggtcgaac gacatgttca tatcctccag tttaatttca
     8341 agtcgcggct tttccagaaa cttatcccgc aactcattca gtgttaatgt gtgtttttcg
     8401 ctacgtaaag tctcttcatc acgaatttcc ttgccgcgat aatcttccga taattgcctt
     8461 acgtatgaag gtaaaactat tcgcatatct aactcaaaag cctcttcgaa aaattctgta
     8521 agatcggttt ctccgctaat acccttgttc cagttgttgt ttttctgata aagaaaaaag
     8581 ccatacaccg ccacagtttc ataagcaaaa gacctgacaa acgaaggagt gttgggataa
     8641 tcttccagtc tcgctatgag gtattcgcgc aactgccatt tatcgcgtcc gctcatcatt
     8701 tgtccggtgt aggttgcaag gccttcaagc aattccagtt tgttctccga tagttgcgat
     8761 ccgctgtata ccagatgacg atactttctg aatatcaatg cattccgcaa atgttcttct
     8821 gccagcgaaa gcctcctggc cctcagtgct ttttttaagg cttcaagttc gagccgaaga
     8881 aataccctgc cgtctttctc atccaggtga taattatctg atccccgtat atcgaagcca
     8941 agcgaaagtt gtgcactgtg gaacagttcg tgcgtaataa gatcgagtcg ctggtattta
     9001 ttgggtgaaa gcggcaaaac gactgttgcc catgtttttc cgttccaatc gagcgaagtg
     9061 ttctgaaaac tgatttcttt gggcaggact cccgtgtaat aacccctcct tgccaccaat
     9121 acattgtctt tgtccggctc attggcatag attacacggg taccttcctc tatgagcata
     9181 atagggccgt agatgtcctt atcccatatt ttaacatttt cgcgtgttgc ctgttttacc
     9241 tgatcaaaat aatgcggaat actgtccctg tttatcatcg gggtttgtgc accggccaca
     9301 gggataaaaa aagaaatcaa tactgcgcaa acaatacctt tcattcctct ccaacatctt
     9361 gaggcgaaag atacaaaaaa catttccttt taaagaaaat tgctcaaaat cttttctttt
     9421 aatctgtaag tctacttcaa aaactccgaa gacttatttt atcgtagaag acttatccgt
     9481 taaaaaacac atggaggtta aaaaggttaa ctttcaggat tcacctcccg aatgaatgtc
     9541 tatatccttt accacaagtg aagccagtgt cattccgaaa attgccggca ggtaagacat
     9601 tgtaccgtta attcgtgctt tttttgtatc ccattgagaa acaggagtga tttgctcctc
     9661 tagaaacggt ttttctcctt tgttttcacg cgcttcattg ctgtagacac aaaggaattt
     9721 tttttccagt cctcccagtt tctttattct tgtccggagt gcggcagcca gaggacaacc
     9781 tttcactttc caaaactcat ctaccctgat gtaggaaggg tcaatcttca acgccgcacc
     9841 catcgatgaa aagaaaactc caggcatttg cgaggctaaa cgtataagat gaactttatt
     9901 ggaaaggctg tcgatagcat caataatgaa atcatacgat tcgagcttaa aggaaccggc
     9961 ggtctccgga ctatacacta cttgcataga aacaacttcg gcattgggat ttatttcgag
    10021 cagtcgttcc tttaacacct ccaccttcac ttgtccgata gttttcaccg tagccatcag
    10081 ctgccggttg atattggtta cggcaaccct gtctgaatcc accagcgtaa gatgggtaat
    10141 cccgcttctt ataagcattt ctgcgcacca gctcccgact cctcccacac caaatataat
    10201 aaccctcttt cgagctgcga gatccaaata acggcttccc ataaggagct cagttcgctg
    10261 aaatatctct ttttcgttta tcataaaaca ggacatgaaa aaattcaaaa cagtttctaa
    10321 caatcgagta cttttcttta ccaggaaatc acttttgcgg aaaaggttat cagccatttg
    10381 cgcggaataa acctgaccag gttggctacg aatttattca atcctccttg aataaccagt
    10441 gtttttcctt tcatcatggc atgataacca aacgctacca cttcccctgg caaaggcgtt
    10501 ttcatgtctt tcagaaatcc gtttgggtgc tgcatccggg cagctatccc aaaattggtt
    10561 ctgacgggtc cgggacaaag tgcagtgacg gtaataccgt atttttggaa ttcgtaagca
    10621 atggcttgtg agaagcttgc cacgtatgct ttggacgcaa aataaaccga catccccggg
    10681 cccggttgaa aggcagctgt ggatgcaata tttaagattt ttcctgattt acgcgggatc
    10741 caatcctgaa caaaaagatg acataaattc gataatgcac ttatattcaa atcgatcatc
    10801 tgtctatgcc tcacccattc cgtatcggca aaatatccaa aatcgccaaa cccggcattg
    10861 ttcaccaagt aatctacctc gattccggcg tcctttattt catcgtatac aaatcgtacg
    10921 ttttccggct ttgtcaggtc tttggtaatc gtccgtaccg atatgttaaa tgtattttcc
    10981 aattcatctt tcaaatcatt caattctttt tcgcttcggg caacgagaat caggtttgtg
    11041 ccctttgaag cgaacagccg ggcaaactca gcaccaagcc ccctcgacgc acccgtaatt
    11101 aatgcagtat ggttcattct tattgatttt ggaacaaaat taaacttttt ttccgtagcg
    11161 cggtcgaaga aaatgaacca attaacgaaa cgttaactgg ctacgcataa aaaatgccgc
    11221 caaactgaaa aaaaaattgg gttctaattg ataggatgca tgccggttgt gaaattagtg
    11281 tgcatccact ttttatgctc ttttccgtcc tttgtctatc cgcagaaaaa taaacaacaa
    11341 gatggtaaat ccccacaacg acgaaccgcc gtaactaaag aatggcaacg ggatcccaat
    11401 tacgggcata atgccaatca ccattccgat gtttaccagc aaatggaaca gaaatatact
    11461 tacaacacca tagccataca cgcggccaaa gattgtggtc tgtcgttccg ccaaagaaat
    11521 aagcctcagt atgaacacgg caaaaagcag gagaataatg gttgaaccga taaagcccca
    11581 ctcttcgcct atcgtgcaga aaataaaatc ggtgtcttgc tcggggacat atttcagttt
    11641 ggtttgtgta ccgtttaaga aaccctttcc agacatcccg cccgatccaa tggctatttt
    11701 cgattgcccc acgtgataac ccgatccacg caaatcctgc tccattccca acgttacctt
    11761 gatgcgcatt tgctggtgcg gttgcaggac atcgttaaaa acatactcac tcgattcaac
    11821 aaacgcgaac gaacccacaa caaacaacaa gatatacaaa taaacctttt tccaaaaccg
    11881 aagcgacaag taagccaggt aaaccgcgac caatcctaat gccacaagcg ccaataaacc
    11941 gggattgaca ggaagattaa aaaaatgcat aatccatccg gctgccagga caatcaatcc
    12001 tagtccgatg atgatgaaag ccgtttctcg gcgttttacg tagttccaga ccatggcggc
    12061 agcaaaaaca ataatcagta taactgcgat gaactcgccg acggacaaca tccccagttg
    12121 aacgtccgaa aaacggatgg ccagtacaaa atataaaatt gccgccacac ccatgaaaag
    12181 gattccacca ggtaatccct cacggtacaa cacaaaaata aggctcaggt aaaccagtgc
    12241 ggtacctgtt tcactttgca ggatcaccag caccaacgga agaaaaatta aggagaatac
    12301 gataatcagg tttttccttt ccatcaaacg gaaattgtac acgttgaaaa tctttgccag
    12361 cgtaagtgct atgataaact tcataaattc ggcaggctgc aaccgtaccg gacctatcac
    12421 caaccacgaa cgcgaacctt tgatttcggg cgcgacaaca agggtaatga ccaacaatgc
    12481 gattccggcg atgtaagcta caaaggcata cgtttcgtaa aatgaagtat cgattttgag
    12541 gagggcaaac gcaagcagca acgaggtacc catccaaaca agttgcatcc cggcccggcc
    12601 ggaaaggtcg aacatgctca ccgcattctc caggtcgaag ctggccgcat aaatgttcaa
    12661 ccaacccagc actataaaga gtaggtacat aagcgcagta aagcggtcta cccttccctt
    12721 ttcctgtatt tccctagttc ctctgtacat ttctaacttg agttgctgtg gtatcgatgc
    12781 gttgggcaga agcatttctt ctatacgcaa acggtaaaat cacccggctg gcaatagttt
    12841 tttccaacac ctggtcctgc ggcataattt ctcccatcaa gtacttctgc atcattaatc
    12901 ttccaatagg aacggcgttc gtggctccaa agccgccgtt ttccacaatc acggcaatgg
    12961 ccactttcgg gtcgtccttc ggagcaaacc cgatgaacag ggagtgatct tccccgtgcg
    13021 ggttttcggc tgttcccgtc ttgccgcaaa ccgtcaagaa cggctccata tttgccctcc
    13081 ggcaggttcc gtgtgttacg gcatcggcca ttcccgcaac cgtcagttca aaatgctcgc
    13141 ggcggatgtt cgactctctt cgtttgctgt agagcgtatc taacggagcg ccttttctgg
    13201 ctttcaccac atgcggttta tgataataac ccctgttggc gattagcgcc gtataattag
    13261 caagctgcag gggagtggcc aatatttctc cttgtccaat agcaatagaa atgatgttat
    13321 gagccaccca tctatcgttc ttaaatgc
//
LOCUS       DAOSDR010000025        13085 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrimonas sp. isolate AMR_MDS_1826
            NODE_1849_length_13085_cov_4.216731, whole genome shotgun sequence.
ACCESSION   DAOSDR010000025 DAOSDR010000000
VERSION     DAOSDR010000025.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863355
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrimonas sp. (wastewater metagenome)
  ORGANISM  Petrimonas sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Dysgonomonadaceae; Petrimonas.
REFERENCE   1  (bases 1 to 13085)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13085)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:10:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,720
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 35
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13085
                     /organism="Petrimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1849_length_13085_cov_4.216731"
                     /isolate="AMR_MDS_1826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2023866"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(73..1647)
                     /locus_tag="PLI30_01885"
     CDS             complement(73..1647)
                     /locus_tag="PLI30_01885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019540426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HOI78334.1"
                     /translation="MTLQQEIQRRRTFAIISHPDAGKTTLTEKLLLFGGAIHVAGAVK
                     SNKIKKTATSDWMEIERQRGISVATSVMGFEYGNYKINILDTPGHQDFAEDTYRTLTA
                     VDSVIVVVDIAKGVEAQTRKLMEVCRMRHTPVMIFVNKLDREGKDPFEILDELEEELQ
                     VAVRPLSWPIDMGERFKGVYNLYQNSLDLYQPSKQIVTESVHLNIQSPEVERHIGAKL
                     SEKLRSDVELISEVYPGFNREEYLAGKLAPVFFGSALNNFGVKELLDCFVEIAPSPRA
                     VQTEERVVDPYEESFSGFVFKIHANMDPNHRSCIAFVKVCSGRFERNVNYKHVRYSRL
                     MKFSSPTAFMAQKKEILDEAFAGDIVGLPDNGNFKIGDTLTAGEDLHFKGLPSFSPEM
                     FKYIENADPMKSKQLQKGVEQLMDEGVAQLFTNQFNGRKIIGTVGQLQFEVIQYRLLH
                     EYGAQCRWEPINLYKACWIESEDAAQLEDFKKRKYQYMAKDKEGRDVFLAESNYLLMM
                     AQQDFKNIAFHFNSEF"
     gene            complement(1644..2582)
                     /gene="rfbD"
                     /locus_tag="PLI30_01890"
     CDS             complement(1644..2582)
                     /gene="rfbD"
                     /locus_tag="PLI30_01890"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019540425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008831 - dTDP-4-dehydrorhamnose reductase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HOI78335.1"
                     /translation="MAIYTGSNKHLGGTETVTRKQTVFYGLVQKNVLVTGANGQLGQE
                     LKQLAEKTNSPFRFIYTDADVLDITDAGQVQHFVSEYSIEYVINCAAYTAVDKAEADE
                     EVAYRINCTGAENLARSGAKIIHISTDYVFDGTASTPYKEDASTNPLSVYGKSKLKGE
                     EAIWEFAEEWIIIRTSWLYSEFGNNFVKTMLRLMNERDKLNVVADQYGTPTYAADLAE
                     MILVILECEEWKSGVYHFSNLGETTWFKFAEKIKELAAVGRCRLDPVPACEYKTAAVR
                     PMYSVLDKSKIQSAFRVVIPQWENGLERCIEKLTNR"
     gene            2623..3432
                     /locus_tag="PLI30_01895"
     CDS             2623..3432
                     /locus_tag="PLI30_01895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010800647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOI78336.1"
                     /translation="MANFHIIETGYFWADGGAMFGPIPKKYWHKRYPSNHNNMCLMAM
                     RCLFIETDRHKILVDCGAGDKQLNKLKFYQPHNLKDLRAEIGKIGYSPDEVTDVVLTH
                     LHFDHCGGGTVFNGKQEVVPAFPRATYWLSRAQWDNYRKPMLYEAASFFQENIEPVFE
                     AGQLRLIESEQPLCENVTLKLYDGHTPGQIVLFFENEGEKIVFPADVVPTSAHLPLGW
                     LSAYDNNAALAMEEKKRFLDEAKAQNATLIFFHDAYSQMEKMMPSPHQNNR"
     gene            complement(3362..4225)
                     /locus_tag="PLI30_01900"
     CDS             complement(3362..4225)
                     /locus_tag="PLI30_01900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009136478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase family protein"
                     /protein_id="HOI78337.1"
                     /translation="MIKRLSFSSLFLIGLSAVLYASKVDTVNVFSPSMQKEIKTVVII
                     PDAYLQETEFPVLYLLHGYSDNYGGWVNKVPAVKELADWHNMIIVCPDGAYGSWYFDS
                     PVDPAFKFETFVSKELVDWVDKNYKTVRKREGRAITGLSMGGHGGLYLGFRHQDVYGA
                     CGSMSGGVDIRPFPNNWDLAKRLGTQRENPDNWEKNTVMNQLHLLTPNSSRIIIDCGS
                     GDFFYEVNVRLHQELLYRNIPHDFISRPGVHNWEYWANAIKYQALFFNDYFDAGKASS
                     SPSANTHRGRR"
     gene            4339..4677
                     /locus_tag="PLI30_01905"
     CDS             4339..4677
                     /locus_tag="PLI30_01905"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019540326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOI78338.1"
                     /translation="MDIQFKSNSNGTGSFFIEKEGRQIAELDFEVKNNLLNAYHTGVR
                     PELEGQGIAGKLFDELVKYARKNGYKVIPSCSYILAKFRRNPEGFKDIWHRTDDEPTG
                     DACGVKPKGF"
     gene            4735..5331
                     /locus_tag="PLI30_01910"
     CDS             4735..5331
                     /locus_tag="PLI30_01910"
                     /inference="COORDINATES: protein motif:HMM:NF024937.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4126 domain-containing protein"
                     /protein_id="HOI78339.1"
                     /translation="MELNVETISAVALGIGLSASTGFRVFIPLLVAGLASHFGILPLG
                     ESFSWMSSVPALLSFGVAAVVEVLAYYIPFVDNLLDSIATPLSVAAGTLLMTSVFPSD
                     SEWMKWIVGFVVGGGAAATIQSGSAVTRLLSTKFTAGAGNPVVSTGEGVAATGFSILS
                     LFTPILVAVILVIFVVVVLRLVYKNLLKKRKLTSHQGD"
     gene            5377..5937
                     /locus_tag="PLI30_01915"
     CDS             5377..5937
                     /locus_tag="PLI30_01915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI78340.1"
                     /translation="MKISFKLLIILLLPLFCAQANAQQTAYTRDGTKVFLYDDGTWEY
                     AHQTENPADIEINESGSGMSAEIIINDDLLFSIHNGMLDNFEVLRRNGVRLYDNMSGK
                     LRKIGPYDLEYEFSTDRLKQVGPHRIEYDFSSGRIIRIGNYRIEYDFRTNKISRVGDT
                     LFEYSFFNGKLTDIKGHTPGVRITIF"
     gene            complement(5954..7099)
                     /locus_tag="PLI30_01920"
     CDS             complement(5954..7099)
                     /locus_tag="PLI30_01920"
                     /inference="COORDINATES: protein motif:HMM:NF012470.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase"
                     /protein_id="HOI78341.1"
                     /translation="MKQRQVTIALAAGMMLLCSFSSFAQEKTKDVTLYQGGEAYAVKS
                     FPSSFVNKTPKNVILFIGDGMGVSQLFAGLTANRGSLFIENCKYIGFSKTSSADRYVT
                     DSAAGATAISTGTKTYNGAIGVGPDKNPIKTILEEASEQGKATGLVSTSSITHATPAS
                     FIAHQPSRSQEEDIATDFLKTDIDVFIGGGYDFFANRADGRNLLHDLVRKGYAVEREL
                     SGIENFKGKKLAGLTAPKGNPRVSERGNMLPVSTQTAIRVLQQNEKGFFLMVEGSFID
                     SGGHGNNTVQVVEEVLDFDQAVGKALEFAAGNGETLVIVTADHETGGMAILDGSVETG
                     MVKGGYATGGHTGVMVPIFAYGPGASEFIGIMENTDIHAKIRNFLLK"
     gene            complement(7111..7920)
                     /gene="nagB"
                     /locus_tag="PLI30_01925"
     CDS             complement(7111..7920)
                     /gene="nagB"
                     /locus_tag="PLI30_01925"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007751131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004342 - glucosamine-6-phosphate
                     deaminase activity [Evidence IEA];
                     GO_process: GO:0006046 - N-acetylglucosamine catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HOI78342.1"
                     /translation="MRLIIQSGANQMAQWAANYIAAKINKANPTSEKPFVLGLPTGSS
                     PLLTYKALIKLYESGVVSFENVITFNMDEYIGLPKDHPQSYYTFMWQNFFNHIDIKKE
                     NVHILNGMAEDPEAECRAYEDAMKDAGGVDLFLGGIGPDGHIAFNEPGSSLSSRTRIK
                     TLTTDTVIANSRFFDNDVNKVPKTALTVGVGTVLDAKEVLILVNGHHKARALYHAVEG
                     PINQMWTISALQLHQKGIIVCDYDACAELRVGTYKYFLDIEHDNLDPESLL"
     gene            complement(7965..9197)
                     /locus_tag="PLI30_01930"
     CDS             complement(7965..9197)
                     /locus_tag="PLI30_01930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019540321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FprA family A-type flavoprotein"
                     /protein_id="HOI78343.1"
                     /translation="MYKPFEIKENLFYVGVNDRTKHLFENLWPLPKGVSYNSYIINDE
                     KIALIDTVDICYADVFFNKIRSVLGDKQIDYLIVNHMEPDHSGSVELLKTRYPQIKIV
                     GNSRTAEMLKGYYGITDNVVVIEDKEELNLGKNNLQFYLTPMVHWPETMMTYVKESKT
                     LFSADAFGTFGTLDGGVTDIQLNPERYWDEMIRYYSNIVGKFGSPVQAALKKLSGMGI
                     ETICSTHGPVWTQPDTLGKVVSLYDRLSRYESENGLVIAYGSMYGNTEQLAEIIAAAA
                     AENGARNIIMHNVSKSHESEVLRDIFKYRGLIIGSPTYNNKLYPAVESLLSALQNRNV
                     KNKFFSFFSGHTWADGAKRELKAFAEGMEFETICESVEMKQSLNRNVMENAYALGKAM
                     AERLHSGDAVIPHKTTCH"
     gene            complement(9256..10155)
                     /gene="lgt"
                     /locus_tag="PLI30_01935"
     CDS             complement(9256..10155)
                     /gene="lgt"
                     /locus_tag="PLI30_01935"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019540320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HOI78344.1"
                     /translation="MNTLLTVTWNVDPTIFTVLSREIRWYGLLWVIGLIVAVYIVQRI
                     YKSEKLPEKWFDSLFVYMMLGIILGARLGHCLFYEPEYYLANPVEMLKIWEGGLASHG
                     GVIGIIIAVWLYSKRVTKKSMLWTFDRVMVPTGFTAAMIRFGNLMNHEIYGGPTDLPW
                     GFRFIDNVGLWMRGAEPVYTEPSHPTQIYEALIYLLVFGITMYMYWKTNAKNRQGLIL
                     GVGIALIFIARFFIEFVKNVQVDSEIAMRENTGLILGQWLSIPFIIWGLWLIWRSMKR
                     KAELPVQKEKINADYSQNVKPKK"
     gene            complement(10187..11083)
                     /locus_tag="PLI30_01940"
     CDS             complement(10187..11083)
                     /locus_tag="PLI30_01940"
                     /EC_number="1.4.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005859329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047850 - diaminopimelate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0009085 - lysine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate dehydrogenase"
                     /protein_id="HOI78345.1"
                     /translation="MKKIRAAVIGYGNIGKYVMESLIDAPDFEITGVVRRDATNVPAE
                     LKGFEVVNSISQLKNTDVALLCTPTRSVEKFAKECLALGINTVDSYDIHGGIVDLRHS
                     LNDVAKQHNAVSIVSAGWDPGSDSVVRTLMEAMAPKGITYTNFGPGMSMGHTVAVKAI
                     EGVKAALSMTIPTGTGVHRRMVYIELNDGYDFDQVAKAIQSDDYFAHDETHVFRVENV
                     EALKDMGHGVLMERKGVSGNTQNQLFRFDMRINNPALTAQVMVGCARAAVKQKPGAYT
                     LIEIPVVDLLPGDREKWIKKLV"
     gene            11338..12336
                     /locus_tag="PLI30_01945"
     CDS             11338..12336
                     /locus_tag="PLI30_01945"
                     /inference="COORDINATES: protein motif:HMM:NF013335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HOI78346.1"
                     /translation="MKALLVYLLIPLLVFFSCNSNPQEGAVASPEKIIFDTDMGPDYD
                     DVGAIAILHALADLGECEILATLASDGHPSIAPTIEMFNRYYGKGGIPVGIPDNGVAN
                     FTASNNWNDSLLVRFAPDLKSKTDYPKASEIYRKVLASQPDKSVTIVTVGFTTNLAEL
                     LKTGNDDYSPLSGVELVKKKVKKWVAMAGRFPEGREFNVFIDSVSSYVLERWPTPILF
                     SGFEIGERIFTGSKVAEKNDQNNPVSWAYKYNLATYENKPVKNRMSWDQTAVLCAIRD
                     PERYFYVCGPGKIIVNSSGYNNWDPDSTKDHYFLVHKYPYQDISDTLEGLMMYQPK"
     gene            complement(12344..13012)
                     /locus_tag="PLI30_01950"
     CDS             complement(12344..13012)
                     /locus_tag="PLI30_01950"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019540305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HOI78347.1"
                     /translation="MDNATIHTERWQKKRIRLATIPALVICVLVWLTFLIDYARIFRF
                     NFSLLGIYPRQLNGLAGIVFSPFIHGSLSHLVSNTFPMLVLFSLLFYFYNQIAFKSLT
                     LLWLLSGFFTWLIGRNSYHVGASGLIFALVFFLFFSGLFRKYIPLVAVSMVVAFVYGS
                     TVWSIFPITEYIDVNLSWEAHLSGAAAGLIVAFVFRSRGPQKPEVLWEEDGEDDNDET
                     VITG"
BASE COUNT         3534 a         3129 c         2793 g         3629 t
ORIGIN      
        1 catgaatgcc tcttttctga tgaaaagcat gggcattacg gaatagcgga tgaagaatcc
       61 tggaaatgtt cgtcagaact cggaattgaa atggaaggca atgtttttaa agtcttgctg
      121 cgccatcatt aacagatagt ttgattcggc cagaaacaca tccctaccct ctttatcttt
      181 agccatgtac tgatatttac gctttttgaa atcttccagt tgcgccgcat cttcactttc
      241 tatccagcac gctttataaa ggttaatggg ttcccaacgg cattgcgcac cgtattcgtg
      301 caaaaggcgg tattggatta cttcaaactg aagctgtccg acggttccga tgattttgcg
      361 gccgttaaac tggttggtga aaagctgtgc caccccttcg tccatcagtt gctccacccc
      421 tttctgcagt tgtttcgact tcatcgggtc ggcattttcg atgtacttga acatctccgg
      481 cgagaagctg ggtaatccct taaaatgaag gtcctctcca gcagtgaggg tgtcgccaat
      541 tttaaagttt ccgttgtcgg gcaagcccac gatatctcct gcaaaagctt cgtccagaat
      601 ctcttttttc tgagccatga aggccgtggg cgaagaaaac ttcattaacc ggctataccg
      661 cacatgcttg taattgacgt tgcgttcgaa cctgcccgaa cagaccttca caaaagctat
      721 gcacgaccgg tgattgggat ccatattggc atgtatctta aagacaaatc cggaaaagga
      781 ctcttcgtag gggtccacca cacgctcttc ggtctgtacg gcccgcggtg acggagcaat
      841 ctccacaaaa caatcgagca actcttttac cccgaaattg ttgagtgccg aaccaaagaa
      901 gacaggcgcc agtttcccgg caaggtactc ttcccggtta aatccgggat acacctccga
      961 tataagctcc acatcgcttc gcagcttttc cgaaagcttt gccccgatat gcctctctac
     1021 ttccggcgat tgaatattca agtgcacgga ttccgtaaca atctgtttac tcggttgata
     1081 caagtcgagg ctgttctgat agaggttata cactcctttg aaacgttcac ccatatcgat
     1141 cggccagctc agtggccgta ccgctacctg gagctcttct tccagttcat ccaggatctc
     1201 gaacggatct tttccctcac ggtccagctt gttcacaaag atcatcaccg gcgtatggcg
     1261 catacggcac acctccatca gcttgcgggt ttgtgcttcc acacccttgg caatatctac
     1321 caccacaatt acgctgtcca cagctgtcag tgtacgatag gtgtcttctg cgaaatcctg
     1381 gtgcccgggt gtatcgagaa tattgatttt ataattgccg tactcaaacc ccatcacgga
     1441 agtagctacg gaaatcccgc gttgcctttc gatctccatc cagtcggatg tggcggtttt
     1501 ttttatcttg tttgatttta cagctcccgc cacgtgtatg gccccgccaa aaagcaacag
     1561 tttctccgtt aatgtggttt ttcccgcatc gggatggctg atgatggcaa acgttcgccg
     1621 gcgttgaatc tcttgttgta atgtcatctg tttgtaagct tttcaatgca tctctccagc
     1681 ccgttctccc attgcggaat aacgacacgg aaagccgatt gaatttttga tttatccaat
     1741 acgctgtaca ttggccgtac agcggctgtt ttgtattcgc aggcaggaac gggatctaac
     1801 cggcaacggc ctacggcagc caactctttt attttttctg caaatttgaa ccaggtggtt
     1861 tcccccaggt tggaaaaatg ataaacgccc gatttccact cctcacattc aaggatgacc
     1921 agaatcatct ctgccaggtc ggcggcgtag gtaggtgttc cgtattggtc ggcaacgacg
     1981 tttagcttat cgcgctcatt catgaggcgg agcattgtct tcacgaaatt gtttccaaat
     2041 tcggaataga gccacgaagt acggataatt atccactctt cggcaaactc ccaaatcgcc
     2101 tcttcgccct tcagtttcga ttttccgtaa accgacaacg gattcgtgga ggcatcctct
     2161 ttatagggag tactggccgt gccgtcgaac acataatcag tagagatatg aattattttt
     2221 gcacccgacc tggccaggtt ttccgcaccg gtgcaattga tcctgtacgc cacttcctca
     2281 tcggcttcag ccttatccac agccgtatag gctgcacagt tgatgacgta ctcaatggag
     2341 tattccgata caaagtgctg tacttgcccc gcatcggtaa tatccagcac atctgcgtcg
     2401 gtatagataa accgaaaggg ggaatttgtt ttttctgcca gttgtttcag ttcttgtccc
     2461 agttgtccgt ttgcaccggt gaccaaaacg tttttttgaa ccagtccgta aaagacggtc
     2521 tgtttccggg tgacagtttc cgttccgcca agatgtttat ttgatcctgt gtagatagcc
     2581 attacacttt tctttttact tttgcacaaa agtactacga aaatggcaaa tttccacata
     2641 attgagacag gttatttctg ggcagatggc ggtgccatgt ttgggcccat acccaaaaaa
     2701 tactggcaca aacggtatcc aagcaaccat aacaacatgt gcctgatggc aatgcgatgc
     2761 ctcttcattg aaaccgaccg acataaaata ctggtagatt gtggggctgg cgacaagcaa
     2821 ttgaacaagc tgaagttcta ccagccgcac aatttgaaag acctgagggc agaaatcgga
     2881 aaaatcggct attcgcctga cgaagtaacc gatgttgtac ttacccacct ccattttgat
     2941 cactgcggcg gaggaacggt ttttaacggg aaacaggagg ttgtccctgc ttttccccgg
     3001 gccacttact ggctgagtcg ggcgcaatgg gataattaca gaaaaccgat gctttacgaa
     3061 gcggcttcgt ttttccagga aaacattgaa cctgtcttcg aggcaggaca actccggctg
     3121 atagaatcgg aacaaccgct ctgtgaaaac gtaaccctca agctctacga tggacataca
     3181 cccggacaga tagtcctctt tttcgaaaat gagggagaaa aaatcgtttt ccccgcagat
     3241 gttgtcccta cttcggcaca tctgccgctc ggctggttat cggcctacga caataacgcc
     3301 gcactggcca tggaagagaa aaaacgtttt ctggacgagg ctaaggccca aaatgcgacg
     3361 ctcatcttct tccacgatgc gtattcgcag atggagaaga tgatgccttc cccgcatcaa
     3421 aataatcgtt gaaaaacaac gcctggtatt taatggcgtt tgcccaatat tcccagttat
     3481 gtacaccggg gcgcgagata aaatcgtggg gtatattccg gtaaagaagt tcctgatgta
     3541 acctgacgtt tacttcgtaa aagaaatcac cggaaccgca atcgattatt attctggagg
     3601 agttgggcgt aagcaaatgc aactgattca ttacggtgtt cttctcccag ttatcggggt
     3661 tttcacgttg cgtgcccaac cgttttgcca gatcccagtt gttgggaaaa ggacgaatat
     3721 ctactcctcc actcatactt ccacaagcac cgtagacatc ctgatggcga aatcccaggt
     3781 acaatccgcc gtgaccaccc atacttaatc cggtaatggc gcgtccttcg cgttttcgga
     3841 cggtcttgta gttcttgtcc acccaatcca ccaattcttt cgatacaaaa gtttcaaact
     3901 taaatgcagg atccacggga ctgtcgaaat accagctccc gtaagctcca tcgggacaaa
     3961 cgataatcat attgtgccaa tcggccaact ctttcaccgc aggcactttg ttaacccacc
     4021 caccgtaatt gtcactgtaa ccgtgtaaca aataaagaac cggaaattcc gtttcttgca
     4081 aataagcatc gggaatgatt accaccgttt tgatttcttt ttgcatagag gggctgaaga
     4141 cattgaccgt atctactttt gaagcgtaaa gcactgctga taaccctatt aaaaacaggc
     4201 tgctgaatga aagacgtttt atcatgatcg tgtgtgaata aattttttcc aaagataacc
     4261 atttgaccgc aattgacatg aattttttga acccattgac gaagcaattg ttatacctga
     4321 aattaatgat ttaaagaaat ggatatacaa tttaaaagca acagcaacgg cacaggaagt
     4381 ttttttatcg agaaagaagg caggcagatt gccgaactcg attttgaagt gaaaaacaat
     4441 cttttaaacg cttatcacac cggcgtccgt cccgaactgg aagggcaggg aattgccggt
     4501 aaattgtttg atgagctggt aaagtatgcc cgcaaaaacg gatataaagt cattccgtcg
     4561 tgttcgtata tcctggcaaa attccggcga aaccccgaag ggttcaagga tatctggcac
     4621 cgcacagatg atgagcccac gggagacgca tgtggtgtta aaccaaaagg tttttagtaa
     4681 gtcaaaaaaa cgttttaagc atacggaaaa cttttaaaaa cacctccttt aactatggaa
     4741 ttaaacgtag aaaccatcag tgccgtggcg ctcggcatcg gattgagtgc cagtaccggg
     4801 ttccgggtgt tcataccgct tctggttgcc ggattggctt cgcattttgg gattttgccg
     4861 ttgggggaga gcttttcgtg gatgagttct gttccggcat tgttaagctt tggcgttgcg
     4921 gctgtcgttg aagtattggc gtattatatt ccttttgttg acaacttgct ggacagtata
     4981 gccacgcctt tatcagtggc agcaggcacg ctgttgatga catcggtttt cccttccgac
     5041 agtgagtgga tgaaatggat agtagggttt gtcgtgggcg ggggagcagc tgccaccatt
     5101 caaagcggaa gtgccgtaac ccgccttcta tcgactaaat tcactgccgg ggcaggaaat
     5161 cccgtcgttt caaccggaga aggcgtcgcc gccaccggtt tttcaatcct gtcgctgttc
     5221 acgcccatcc tggttgccgt tatcctggtc atttttgtgg tggttgtatt gcggctggta
     5281 tataaaaatt tgctcaaaaa aagaaaatta acatcacatc agggagactg agtttcttgt
     5341 gtcccggatg acatacaatt acaacattag aaccgcatga aaatatcatt taaactactg
     5401 attatactat tattgccgtt attctgcgct caagcaaatg cacaacagac agcatacacc
     5461 cgggacggca ctaaagtatt tctatacgac gacgggacat gggaatatgc ccaccaaact
     5521 gaaaatcccg cggatataga aattaatgaa tcaggaagcg gaatgtcggc tgaaataatc
     5581 atcaacgatg atttactgtt cagcatacat aacggcatgt tggataattt cgaggtcttg
     5641 agaagaaatg gagtccgact gtatgacaat atgagtggaa agttaagaaa gataggtcct
     5701 tatgacctgg aatacgagtt ttccacggat aggttgaaac aggtgggacc gcacaggatt
     5761 gaatatgatt tcagttcagg aagaattatc cggatcggga attaccgcat agaatatgac
     5821 ttccgtacaa acaaaatatc acgcgtagga gacaccttgt ttgaatacag tttctttaac
     5881 ggtaaactta ccgatataaa ggggcatacc cctggtgtaa ggataactat tttctgaaat
     5941 tatcccgaag cttttatttc aacaaaaaat tccggatttt ggcatgaata tcggtatttt
     6001 ccataattcc gataaactcc gatgctcccg ggccgtaagc aaagatggga accatgactc
     6061 ccgtatgtcc gcccgttgca tagccaccct ttaccattcc cgtttcaacg gaaccgtcaa
     6121 gaatagccat ccctccggtc tcgtggtcag cagtaacgat gactaacgtt tccccgttac
     6181 cggcagcaaa ttcaagcgct ttacctacgg cctggtcaaa gtccaacacc tcttcaacca
     6241 cttgaacggt gttatttccg tggccgccag aatcgataaa tgaaccttcc accatcagaa
     6301 aaaaaccttt ttcattctgc tgcaaaacac gaatagctgt ttgtgtggaa accgggagca
     6361 tattcccgcg ttccgatacg cgcggattcc cttttggcgc cgttaggccg gctaattttt
     6421 tccctttgaa attttcaatt ccgcttaact ccctttctac tgcatagcct ttacggacaa
     6481 ggtcatgcaa caggttacgc ccgtccgctc tgttagcgaa gaaatcatac cctccaccga
     6541 taaaaacatc aatatcggtt ttgagaaagt cggtagcaat atcttcctcc tggcttctgc
     6601 tcggctgatg tgctatgaag gatgccggtg ttgcgtgggt aatggaagag gtggatacca
     6661 gtccggtcgc ttttccctgt tccgacgctt cttcaagaat tgtttttatc gggttcttat
     6721 cgggccccac acctatagct ccattgtagg ttttggttcc cgtggaaatg gcagtggctc
     6781 ctgctgccga gtcggttacg taccggtcgg ccgacgaagt ttttgagaac ccgatatatt
     6841 tacaattttc gataaacaaa cttcccctat ttgcggtgag ccccgcaaaa agttgcgaaa
     6901 ctcccatccc atccccaata aaaagaataa cgttcttcgg tgttttgttt acgaatgacg
     6961 aggggaagga tttaacggca tacgcttccc caccctgata caacgtaacg tctttggttt
     7021 tttcctgtgc aaaagagctg aagctgcaaa gcaacatcat tcccgcagcc aatgcgattg
     7081 ttacctgtcg ttgtttcata atctactttg ttataacaat gattcgggat ctaaattgtc
     7141 gtgttcaata tccagaaaat acttataagt gcctactctc agttcggcac aggcatcgta
     7201 atcgcagacg ataatccctt tttgatgcaa ctgcagcgcg ctgatcgtcc acatctggtt
     7261 gatggggccc tcaacagcat gatacagtgc acgcgctttg tgatggccgt tcaccagaat
     7321 caacacctct ttagcatcga gaaccgtccc cactcccact gtgagagccg tcttaggcac
     7381 cttgttcaca tcgttatcga aaaagcggga attggcgatg acggtatcgg ttgtgagcgt
     7441 tttgattcgt gtacgtgagg aaagtgacga ccccggctca ttaaaggcga tgtgtccgtc
     7501 ggggcctatc ccaccgagga acaagtctac gccaccggca tctttcatgg catcttcgta
     7561 cgccctgcat tcggcttccg gatcctccgc cataccgttg aggatatgca cattttcctt
     7621 cttgatatcg atatggttga aaaagttttg ccacatgaac gtgtaatagc tctgcggatg
     7681 gtctttgggt aaacctatgt attcatccat attgaaagtt atcacgttct caaacgacac
     7741 cacgcctgac tcgtacagct taatcaacgc tttgtatgtc aaaagcggtg aagaaccggt
     7801 aggaagcccc aatacaaagg gtttttccga agttggattc gccttgttaa tttttgcagc
     7861 gatgtagttt gctgcccact gtgccatttg gttggcaccg gattgaataa ttagtcgcat
     7921 atatttttaa aaaataataa gttataattg caaatcgtta atggtcaatg acaggtagtc
     7981 ttatgcggta tgacggcatc gcccgaatga agcctctcag ccatagcctt acctaacgca
     8041 tacgcatttt ccatcacgtt tctgttcagt gactgcttca tctccaccga ctcacaaatc
     8101 gtttcgaact ccatgccttc agcaaatgct ttcaactctc ttttggctcc atctgcccag
     8161 gtatgtccac tgaaaaaact gaaaaattta tttttcacgt tccggttttg taaagccgac
     8221 aacaagcttt ccacagccgg gtaaagttta ttgttgtacg tgggtgagcc gattatgagc
     8281 cctcgatatt taaagatatc cctcaacact tccgactcgt ggctcttgga aacgttgtgc
     8341 ataatgatgt tcctggcgcc gttttcggct gccgctgcgg caatgatttc tgccaattgt
     8401 tccgtgtttc cgtacatgct tccgtatgca atgaccaatc cgttttcaga ctcgtaccgg
     8461 cttaaccggt cgtaaagcga caccactttc ccaagggtat cgggttgcgt ccatacgggg
     8521 ccgtgagtag aacaaattgt ctcgatcccc atcccgctca gttttttcag agcagcctgt
     8581 acaggagatc cgaatttccc tacgatgttg gaataatacc ggatcatttc atcccagtat
     8641 cgctccggat tgagttgtat atccgttacc cctccgtcca gagtgccaaa tgtaccgaaa
     8701 gcatctgcag agaacaatgt ttttgattct tttacgtaag tcatcattgt ctccggccaa
     8761 tggaccatgg gggtcaggta aaactgaagg ttgttttttc ccaggttcag ttcttcttta
     8821 tcttctatga caactacatt atcagtaata ccgtaatacc ctttcagcat ttccgctgta
     8881 cggctgtttc ccactatttt tatctgggga taacgggtct taagcagttc cacggaaccg
     8941 gaatgatcag gctccatatg attgacgata agataatcga tttgcttgtc ccctaaaacg
     9001 gaacggattt tattgaaaaa aacatctgca taacagatat ccacggtatc tatcaacgca
     9061 attttttcgt cgttaatgat gtacgaatta taagatactc cttttggtaa gggccaaaga
     9121 ttttcgaaaa gatgttttgt ccgatcgttt actccgacgt aaaacaggtt ctctttgatt
     9181 tcaaatggtt tgtacattac agtataaagt ttacagttta aaagtttaac gtcttcattt
     9241 ccttcgctct taaccctatt tcttcggttt aacgttttgg ctgtaatctg cattgatctt
     9301 ctctttctga accggaagtt cagctttcct tttcattgac cgccaaatca gccacaatcc
     9361 ccagataata aacggtatgc tgagccattg cccgaggatt aatcctgtat tttcacgcat
     9421 ggcaatttcg gaatccactt gtacattttt aacaaattca ataaaaaacc tggctataaa
     9481 aatcaatgca atgcctacac caagaatcaa tccctgtcga tttttggcgt tcgttttcca
     9541 gtacatatac atggtaattc cgaaaacgag aaggtatatc aaggcttcgt aaatttgcgt
     9601 tggatgagaa ggttctgtat agaccggttc tgcgccgcgc atccataatc ccacattatc
     9661 aataaaacgg aatccccaag gcagatcggt ggggccgccg taaatctcgt gattcatcag
     9721 gttcccaaaa cgaatcatcg cagcggtgaa ccctgtgggg accattaccc ggtcgaaagt
     9781 ccaaagcatg ctctttttgg tgaccctttt tgaatacagc caaacggcaa taataatgcc
     9841 gatgacccct ccgtgacttg ccaaccctcc ttcccagatt ttcaacatct ccacggggtt
     9901 tgccaggtaa tattccggtt cgtaaaacaa acagtgtccc aaccgggcac caagaatgat
     9961 cccaagcatc atgtagacaa aaagtgaatc gaaccacttt tcgggaagtt tttcactctt
    10021 gtagattctc tgtacaatgt acacggccac aattaacccg ataacccaaa gtaatccgta
    10081 ccaacggatt tcacggctta aaacagtaaa aattgtagga tctacattcc atgttacggt
    10141 taataatgta ttcatctgtg ttgaatttgg agcaaagata agaaaattaa accaatttct
    10201 taatccattt ttccctgtcg cctggaagaa ggtctacaac aggtatttct attaatgtat
    10261 aggcacccgg cttttgtttc acagcagccc tggcacaccc gaccattacc tgtgcggtca
    10321 acgcggggtt gtttatgcgc atatcgaacc tgaaaagctg gttctgtgta tttccggata
    10381 cccctttacg ttccatcaac acgccgtgtc ccatatcttt taatgcttcc acgttctcta
    10441 cccgaaaaac gtgtgtttca tcgtgtgcga agtaatcatc ggattgaatg gctttagcta
    10501 cctggtcaaa atcataacca tcattcagct cgatgtaaac catccggcga tgaactcccg
    10561 ttccggttgg gatagtcatt gaaagtgccg ctttaacacc ctcaatcgct tttacagcta
    10621 ccgtatgtcc catgctcatt cccgggccga aattggtata agttattccc tttggcgcca
    10681 tggcctccat caacgtgcgg acaacggaat cgcttccagg atcccatccg gcggaaacga
    10741 tagaaacggc attgtgttgt ttcgcaacgt cgtttaacga atggcgtaaa tctacaatgc
    10801 ctccgtgaat atcataactg tccaccgtgt taattcctaa cgccagacat tctttagcaa
    10861 atttctccac gcttcgcgta ggggtacaga gtaacgcaac atccgtattt ttcaattgcg
    10921 atatcgaatt tacgacttca aatcctttca gctcggcagg cacattcgtt gcatcacgac
    10981 gcacaactcc cgtaatttca aaatccgggg catcgatcaa cgattccatt acgtatttcc
    11041 caatattgcc gtaaccgata acggcagctc tgattttttt cattttctta taattttgat
    11101 gcacaaaaat agtaaaaaat gcggattctt gccggctcat aatcccggat atttctgctc
    11161 aatcacaaat cagtatgtaa aagtctacag tgttgcattt ttttctgtac ttttactttt
    11221 cgaaatacgg acaacctttg tgtcttctat tctcactaaa tataaagtgt tttatttccg
    11281 gggaggtttt tctcgaaagg aaagtggtat tgaatctaac ttttaaatga taataatatg
    11341 aaagcattac ttgtttatct actgataccc ttgttggttt tcttttcctg caattcaaat
    11401 ccccaagaag gagctgttgc ctcacctgaa aaaataattt tcgatacgga catggggccc
    11461 gattatgatg atgtgggagc aatagccatc ctgcatgcct tggcagattt gggggaatgc
    11521 gaaattttag ccacactggc aagtgacggt catcccagta tagcacctac catcgaaatg
    11581 tttaaccgtt actacggaaa agggggcatt cctgttggaa ttcccgataa cggtgtagca
    11641 aatttcacgg catccaacaa ttggaacgac tccctgctgg taagatttgc tcccgattta
    11701 aaatctaaaa cggattatcc gaaagcgagc gagatttata ggaaggtact ggcttcacag
    11761 ccggataaaa gcgtgaccat cgtaaccgtt ggatttacaa ccaacctggc agaattacta
    11821 aaaaccggga atgatgatta ttctccgtta tcgggtgtag aactggttaa aaagaaagta
    11881 aaaaaatggg ttgccatggc cggacggttt cccgagggcc gggaattcaa tgtatttata
    11941 gattcggtct cttcttatgt attggagcgc tggccaacac ccattctttt cagcggtttt
    12001 gaaataggag agcgtatttt taccggaagc aaggtggcag aaaaaaatga tcaaaacaat
    12061 ccggtttcct gggcgtacaa gtataattta gccacatacg aaaataaacc cgtaaaaaac
    12121 aggatgtcct gggaccagac agcagttctt tgcgccattc gcgaccccga aagatatttt
    12181 tatgtatgtg gccccggaaa aattatcgtc aattcctccg ggtataacaa ctgggatccg
    12241 gactcaacta aggatcacta ttttttagtc cacaaatatc cttatcagga catctccgat
    12301 acgttagagg ggttaatgat gtaccagcct aaatgatata aaatcaccct gttatcactg
    12361 tctcatcatt atcatcttca ccgtcttctt cccaaaggac ttccggtttt tgcgggcccc
    12421 tgcttctgaa aacaaaagca acgatgagcc ctgctgccgc ccccgacaaa tgggcttccc
    12481 acgaaaggtt tacatcgata tattcggtaa tcgggaaaat actccagacg gtacttccat
    12541 aaacaaaagc caccaccatg gatacggcaa caagcggaat atattttcgg aacaacccgc
    12601 tgaagaacaa aaagaaaacc agtgcaaaga ttaaaccgct tgcgcccacg tggtatgagt
    12661 tccgacctat cagccacgta aaaaagccgc tcaatagcca aagtaaagtg agtgacttaa
    12721 aggctatctg attgtaaaaa tagaataaca acgaaaacaa aaccaacatc ggaaatgtat
    12781 ttgagacaag gtgtgaaagc gatccatgta taaaaggcga gaaaactata ccggccaaac
    12841 cgttcagttg acgggggtaa attcccagaa gtgagaaatt aaaacgaaaa atgcgggcat
    12901 aatcaatcag aaaagtcaac cagaccagca cacatattac cagtgcggga atcgttgcca
    12961 gacgaatcct ttttttctgc cacctttctg tatgtattgt tgcattatcc atagatgcaa
    13021 aactaaataa aaagaaccgt ttttcgccgg aaattaccca aaaaacaaaa aagaagcagg
    13081 aagaa
//
LOCUS       DAOSDR010000026        13054 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrimonas sp. isolate AMR_MDS_1826
            NODE_1855_length_13054_cov_3.854835, whole genome shotgun sequence.
ACCESSION   DAOSDR010000026 DAOSDR010000000
VERSION     DAOSDR010000026.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863355
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrimonas sp. (wastewater metagenome)
  ORGANISM  Petrimonas sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Dysgonomonadaceae; Petrimonas.
REFERENCE   1  (bases 1 to 13054)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13054)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:10:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,720
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 35
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13054
                     /organism="Petrimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1855_length_13054_cov_3.854835"
                     /isolate="AMR_MDS_1826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2023866"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            58..801
                     /locus_tag="PLI30_01955"
     CDS             58..801
                     /locus_tag="PLI30_01955"
                     /inference="COORDINATES: protein motif:HMM:NF013782.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase"
                     /protein_id="HOI78348.1"
                     /translation="MDKKRRYQFQVSAQHIDFQKKVSLSSLFHLILKTAGKDADNNGF
                     GLLKLQSDDYTWVLSRFVMDMERFPLENENITIETWIQDVGAMFTTRNFRITNGEDRL
                     IGYVSSSWAVIDMKTRQGVLLDSLASLNAFVWPETIPIGVPTRIANVKGDIANRFEVK
                     YSHIDVNCHASSPFYIQWIADCFSLDFYRRHKLKRFEINFLKEITFGDTGVVYRESKA
                     KNDYLFQLVTSEKGISCRARIQFEDEGNC"
     gene            897..967
                     /locus_tag="PLI30_01960"
     tRNA            897..967
                     /locus_tag="PLI30_01960"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:929..931,aa:Gln,seq:ttg)
     gene            complement(1047..4097)
                     /locus_tag="PLI30_01965"
     CDS             complement(1047..4097)
                     /locus_tag="PLI30_01965"
                     /inference="COORDINATES: protein motif:HMM:NF028442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HOI78349.1"
                     /translation="MKQKTNYLKFFKYRLNAFLAGLILIALSGYCCITGEKETSEKYL
                     EANISENDFCNVPQVDKPWVYYWWLKGNVTKELIARDLREMQKIGIGGFLLLDSRGYY
                     DDYYGKTGHIPVPLDIKYEFMSPGWREMVKFTMQEANRLGLKMSINLANTGGSLRGPW
                     NMKEDGPKQLIWADTTILGPKKISIQLEKPFDKKYFKDVALMAVEIKSDVKSLGKSAG
                     LNLNWNTVSIPAENANIAGEIIDLSNKITNGELNWEIPEGNWRLLRFGHHVIGDEGSV
                     DIFNAEAVTKYFNLMGAEILKDAGPLAGKTLTYFYNVSWEGSQPDWTVNFKKEFKKNR
                     GYDISQFMPILAGMSKADRSINERFLRDYFRTISDCFKHNCYETIGELCHSNGMQWHS
                     ENGGPWPRNASMFKEADQLTFWGINDMPQGEFWCNSINDLQQRSNVKYAANAAHIYGR
                     PLVSVEAFTHMTKHWTMYPSYLKPFADINFIDGANFFIWHTFAASPLEVGKPGYEYFA
                     GTHINPNVTWFNKAGDFFKYLGRCQYMLRKGKYVADYCCYVSDKNYVSWGRGKKWTAD
                     SSLTPVKGTSYDLLNSEVLTDRLSVEDGKLVLPDGVGYNLLVIDLDEPVIPIRVLQKI
                     NELVKDGATLVLGKMVPTRATGLDNYQENDIEVKRLAEELWGNNSDQRQIRSLKKGKV
                     YTGYNLEEVLSDLNIHADFEGPFEYIHRKLDIKDVYFVSGEGKAECTFRVEGMKPEIW
                     NPVTGQIIDAISYYYTKDGRTTIPMDLPENGSVFVVFREKADKNHFVSIDGPEYPEFT
                     EKKEDSTEFIFWESGNYNLKTTNGNSKKLSIRVDPSLELTSAWNITFLPATGVQSFDA
                     SFDKLTLWNDNSDPQIKYFSGTAIYKNIFNLNEDQVKKPANLKLGTVHNISRVWINGK
                     DKGIVWTAPWSVDITGALKEGENELKIEVTNCWANRLIGDAGLPENKWTTQTNVHRVL
                     DRSKYKSGHQAFAATDMLMPSGLVGPVQIKFGEKRSVSLNKASL"
     gene            complement(4565..4900)
                     /locus_tag="PLI30_01970"
     CDS             complement(4565..4900)
                     /locus_tag="PLI30_01970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006843962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegP family protein"
                     /protein_id="HOI78350.1"
                     /translation="MGKFVITTRKSGEFQFNLKAGNGQVILSSEGYSTKTACLNGVES
                     VKKNSQEDARFEKNVAKNGKFYFNLKASNGQVIGNSEMYETEASRENGIKSVKTNAPD
                     AQVDDQTVS"
     gene            complement(5031..8414)
                     /locus_tag="PLI30_01975"
     CDS             complement(5031..8414)
                     /locus_tag="PLI30_01975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009036940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HOI78351.1"
                     /translation="MIKKMKFLTVVFFLLLATVVNAQMTTSSMSGRVTDNEGAVIGAT
                     VVATHTPSGTTYGTVTNVDGRFSLNGMRVGGPYKVEVSYVGMNTIELEGIQLQLGETY
                     PVNVEMSESSEVLNEILVTAQRTKFTTEKTGATTNINTDQITLMPTTSRNIQDIARLS
                     PYASSGMDFSGRDGRNSNFTVDGARLNNNFGLSDRLPGGGNPISLDAIEEMQVVIAPF
                     DVRQSNFIGGGVNAITKSGTNTYKGSAYTYFYNQNMRGNSIGDFTPEGDRPKEGKTIY
                     GATLGGPIIKDKLFFFANFETEKSPQQITKWRGSSNGVAKGDEFISHARQSDLDEFSR
                     LLKERYGYDTGSSTDFPGGVSNLKFLGRIDWNINSTHKLSLRYNYTKNDDWVPTNGNS
                     SDTGYRLNGTDRIGVNSMAFMNSTYSQGSIVKSFTTELNSRLSDAISNQLLLTYTDIN
                     DQRGSKSSPFPFFDIMDGDDVVAAGEDPTNNKNFKPYMSAGYELFTWNNGVKNNVFSI
                     TDNFTYYLTSHKLTAGLSYEHQSALNSYMRNATGYYRFKSFSDFKNNAAPEAFALSYG
                     YDGELNPAGKVTLGQLAAYIQDEWNALDNLKLTYGIRMDNTFFLNDMLRNNAIYSLDF
                     GGKKVDTGKWPDAKFQFSPRVGFTWDVLKDKALVVRGGTGLFTGRLPLVFFTNMPQNS
                     GMIQGASNQIYTTYDKDTGAATGRNPLLDQMYVDGKLMTDVNQMIEKLGLKKTITQEE
                     GTLQSNMAGVDNNFKMPQVWKSSLAVDYQIPVSFPLSVTVEGMFTKYINDIRLLNYNI
                     KDQTQANGYTQFEGPDKRFIYPSNYNYYRNHASYDADHKKNVGVLGVLTNTSEGYGYT
                     GNITINAEPVRNLNLMAAYTHTASYEVSGMPGSNATSAWQGLPTVNGPNFNGAQWSQY
                     VSPHRIIASLNYRINYLGGWTSSEFGVFYQAYSPYRYSYTYSNDMNGDAINQDLIYIP
                     ATKDEIQWKTAEDADRFWDFVEKDSYLSKNKGKYADAYGAYAPMVHRFDLHFAQNFIV
                     RTGKTTNTLQVSLDIMNFANLLNSTWGVNKQMIPAANRGQILKFEGVNKDGVAGNADD
                     KVPYFSFFNAEKVSEIYTRYNDVNSQTWRLQLGLRYIFN"
     gene            complement(8778..10412)
                     /gene="groL"
                     /locus_tag="PLI30_01980"
     CDS             complement(8778..10412)
                     /gene="groL"
                     /locus_tag="PLI30_01980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007654643.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HOI78352.1"
                     /translation="MAKEIKFEMEARDLLKKGVDSLADAVKVTLGPKGRNVILDKKYG
                     APQITKDGVTVAKEIELEDSFQNMGAQLVKEVASKTNDDAGDGTTTATVLAQSIIGVG
                     LKNVTAGANPMDLKRGIDKAVAKVVENLREQAVEIGDDLKKIENVAKISANGDEAIGK
                     LIAEAMQKVSKEGVITVEESKGTDTYVDVVEGMQFDRGYISPYFVTDTENMEVQFENP
                     LVLVHDKKISAIKDLLPILEKGIQTGKPMLIIAEDIDSEALTTLVVNRLRGSLKIAAV
                     KAPGFGDRRKEMLEDIAILTGGTVISDEKGLTLENATLDMLGSAEKITIDKDTTTIVN
                     GEGDKDAISNRIGQIRSQIEKTTSDYDREKLQERLAKLAGGVAVLYVGAPSEVEMKEK
                     KDRVNDALSATRAAVEEGTVPGGGVAYIRASEALEGFKGENEDETTGIEIVKRAIEEP
                     LRQIVANSGKEGAVIVQRVREGKADFGYNARTDKYENLYETGVIDPTKVARVALENAA
                     SIAGMFLTTECVITDKKEENPAPQMPMGGGGMGGMM"
     gene            complement(10492..10764)
                     /locus_tag="PLI30_01985"
     CDS             complement(10492..10764)
                     /locus_tag="PLI30_01985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013617859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HOI78353.1"
                     /translation="MSIKPLADRVLVKPAAAEEKTVGGIIIPDTAKEKPLKGEVISTG
                     NGTKDEEMVVKAGDNVLYGKYAGTEIELDGEKYLIMRQSDILAILS"
     gene            11059..11706
                     /locus_tag="PLI30_01990"
     CDS             11059..11706
                     /locus_tag="PLI30_01990"
                     /EC_number="6.3.1.14"
                     /inference="COORDINATES: protein motif:HMM:TIGR00290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diphthine--ammonia ligase"
                     /protein_id="HOI78354.1"
                     /translation="MKAFVSWSSGKDCMHALYHFLKNPENQAVCLLNMSGEGNGQPHS
                     HGISSAMIRKQAARLNIPLLQKATSRGNYEKNMKEAIARLKSEGVDAGVFGDIYLQEH
                     RTWIERVCGEAGVSAVFPLWDKSTSGLMEQFITERFKTIVVSVRKEKLPKSFLGRLID
                     NDFLTDIHTFPATDPCGENGEYHTFVFDGPLFAAPVLFEKGDITEDDNHWFLDIK"
     gene            11718..12533
                     /locus_tag="PLI30_01995"
     CDS             11718..12533
                     /locus_tag="PLI30_01995"
                     /inference="COORDINATES: protein motif:HMM:NF013648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helical backbone metal receptor"
                     /protein_id="HOI78355.1"
                     /translation="MRKRFFSGMLLLFYVVVSFAQTPYKRIVSLAPSLTQSLYYLDAQ
                     DNLVGCTSYCMAAKEDKKEVVASAVKANLEKVIALKADLVLVSGFTDLQDIETLRKFG
                     IKVEVFQSPKSFQEICGQFIRLGKLIGKEERARQIVLESEQKIKNISSGIKWGRKPRM
                     FFQIGANPLFSVLPNTFMDDYMNFLGAENIAKTLKRGSVSREFVIAQNPDYIFIVTMG
                     IVGKEEKDTWSRYSQLTASRKKQIHIIESDIACQPTPITFVQTMEVLEKIINK"
     gene            12545..>13054
                     /locus_tag="PLI30_02000"
     CDS             12545..>13054
                     /locus_tag="PLI30_02000"
                     /inference="COORDINATES: protein motif:HMM:NF014614.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HOI78356.1"
                     /translation="MNNLGLIHIYTGDGKGKTTASVGLAIRALGSGLKVVYCSFHKKP
                     EKYGYTEIDGLKKLGAQVFTFAKGHPHLDKSIDENAIKREVSEAIDTLNRMLKNDQTD
                     MLILDEILISVRDNYLEENTLIDFIKNKPPHTELILTGRGATPNVMDLADYVSFVRKI
                     KHPYDKGVFS"
BASE COUNT         3763 a         2786 c         2393 g         4112 t
ORIGIN      
        1 ggtaagaaaa actattacct gctgatagcc atataacaat cgcttttccc tgcctgcatg
       61 gataaaaaaa ggaggtatca attccaggtt tcggcccagc atatcgattt tcagaaaaaa
      121 gtatcgttgt cgtctttgtt tcatctgatt ttaaagacag ctggaaaaga tgctgacaac
      181 aacgggttcg gtttgctgaa actgcaatcg gatgactaca cgtgggtcct ttcacgcttc
      241 gtcatggata tggaaaggtt tccactggaa aacgaaaaca taaccattga aacgtggatt
      301 caagacgtgg gtgcaatgtt taccacacgt aattttcgaa tcaccaacgg tgaagaccgg
      361 cttatcggtt acgtgtcatc ttcgtgggcg gtaattgata tgaaaacccg acagggagtt
      421 ctgttggact cgttagcgtc actgaacgct tttgtatggc cggaaactat ccccataggt
      481 gtccccaccc gcattgccaa cgtcaaagga gacatcgcca accgttttga agtaaaatac
      541 agtcacatcg atgtaaattg tcatgcaagc agtccgtttt acattcaatg gatagctgac
      601 tgcttttcgc tggattttta caggaggcat aagttaaaaa gatttgagat aaattttctg
      661 aaagaaataa cgttcggtga caccggagtc gtttacaggg aatcgaaagc taaaaacgat
      721 tatctttttc aactggttac ttcggaaaaa ggaatttcct gccgtgcacg gatacagttt
      781 gaagatgagg gaaactgctg atttggatcc gggagtaaaa aacatctccc aaaaatttgg
      841 tcatttacaa atattgttat acttttgcac tgcttttcac gaagcacatt aaggattgtc
      901 ctatggtgta atggtagcac tcctggtttt ggttcagtca gtctaggttc gaatcctggt
      961 aggacaacaa taaacggcgc atgaagcata ttccagctac ttgcgccgtt taacttgtta
     1021 aaaaggatga attttgagat cttttttcat aaagaggctt tgtttaaact tacagatcgt
     1081 ttttctccaa atttaatttg caccggtccg actaaacccg atggcataag catatctgtt
     1141 gcagcaaatg cctggtgtcc tgatttatat tttgatctgt ctagaacacg atgaacattt
     1201 gtttgtgtag tccatttatt ttccggcagc ccggcgtcac caatcagtcg atttgcccaa
     1261 cagttagtaa cttctatttt cagttcattt tcaccttcct ttaatgcccc ggtaatatca
     1321 actgaccagg gggctgtcca gactattcct ttatctttac cattgatcca aacgcgcgaa
     1381 atattatgaa ctgtccctaa tttcaggttg gctggttttt ttacctggtc ttcattcaaa
     1441 ttgaaaatat ttttatagat agccgttccc gagaaatatt ttatctgcgg atctgaatta
     1501 tcattccaca atgttagttt atcaaaagaa gcgtcaaatg attgtactcc tgtcgccggt
     1561 aaaaatgtga tattccaagc acttgtcaac tctagcgaag gatctactct gatggacaat
     1621 ttttttgaat ttccattcgt tgtttttaaa ttataatttc cgctttccca gaatataaat
     1681 tcagtagaat cttctttttt ttctgtaaac tccggatatt ctggtccatc aatggaaaca
     1741 aaatgattct tgtctgcttt ttccctgaaa acaacaaata cagatccatt ctctggaaga
     1801 tccattggaa tagttgtccg tccgtcttta gtataataat agcttattgc atctataatt
     1861 tgaccagtaa caggattcca aatttcaggt ttcataccct caacgcgaaa tgtacattca
     1921 gcctttccct cccctgaaac aaaatataca tcctttatat ctaatttacg gtgaatatat
     1981 tcaaatggac cttcaaaatc tgcgtgaata tttaagtcgg acaaaacttc ctccaaattg
     2041 taccctgtat ataccttccc tttttttaat gaacgaattt gcctttgatc gctgttgttt
     2101 ccccaaagtt cttccgctaa acgtttcact tcaatgtcat tttcctgata attatcaaga
     2161 ccagttgccc gggtgggaac catttttccc aatacaagag tagcaccatc ttttaccagt
     2221 tcattaattt tttgtaatac acggatcggg ataactggtt catcaagatc gattaccagg
     2281 agattgtagc caacaccatc aggtaaaacc agttttccgt cctctaccga taaacggtct
     2341 gtcaatactt ccgaattaag aagatcgtaa gaagttcctt tcactggggt aaggcttgag
     2401 tcagcagtcc attttttccc tctgccccaa gatacatagt ttttatcgct tacataacaa
     2461 caataatctg ccacatactt tcctttacga agcatatatt gacagcgacc caggtattta
     2521 aaaaaatcac cagctttatt gaaccaggta acatttgggt ttatatgagt acctgcaaaa
     2581 tattcgtacc ctggttttcc tacttccaat ggagaagcag caaaagtatg ccagataaaa
     2641 aagttggctc cgtctataaa atttatatct gcaaaaggtt ttaaataact cgggtacatt
     2701 gtccaatgtt ttgtcatatg agtaaatgct tcaacagaaa caagcgggcg tccatatata
     2761 tgagcagcat tagcagcata tttaacattc gaccgttgtt gcaaatcatt tatgctattg
     2821 caccaaaatt ctccttgagg catatcatta ataccccaaa aggtcaattg atcagcctcc
     2881 ttaaacatag atgcattgcg gggccagggc cctccgtttt ctgaatgcca ttgcattccg
     2941 tttgaatgac atagctcacc aatcgtttcg taacaattat gtttgaaaca atcactgata
     3001 gttcgaaaat aatcacgtag gaaccgctca ttaatggaac gatcggcttt agacattccg
     3061 gcgagaatcg gcatgaattg actaatatcg taacctctgt tttttttaaa ttctttttta
     3121 aaatttactg tccaatcggg ttgtgaacct tcccaactga cattgtagaa gtaagtgagt
     3181 gtctttcctg ccagtggtcc cgcatctttt aagatctcag cccccattag attaaaatat
     3241 ttagttactg cctcagcatt gaaaatatca acgctgcctt catctccaat cacatggtgt
     3301 ccgaaacgca ataaccgcca attcccttcc ggaatctccc agttcaactc accattggtt
     3361 attttattag aaagatctat tatttcacct gctatattag cattttctgc gggtattgac
     3421 actgtattcc aatttaaatt taatccggct gatttcccca atgattttac atctgatttt
     3481 atctcaactg ccataagcgc aacatcttta aaatactttt tatcgaaagg cttttccagt
     3541 tgaatagaaa tttttttcgg ccctaaaatt gttgtatcag cccaaataag ttgttttggg
     3601 ccgtcctctt tcatattcca gggaccacgc agcgaacctc ccgtatttgc aaggttgata
     3661 ctcattttta aaccaagacg attcgcttcc tgcatagtga atttcaccat ttccctccaa
     3721 cctggactca tgaattcata ttttatgtcg agaggtacag gaatatggcc agttttccca
     3781 tagtaatcat cataataacc acgtgaatct aacaataaaa atcctcctat cccaattttt
     3841 tgcatttctc tcaaatcacg ggcaatcagt tccttggtaa catttccttt cagccaccag
     3901 taatagaccc atggtttgtc gacttgtggg acattacaga aatcattctc ggaaatattt
     3961 gcttcaaggt atttttcact tgtctctttt tctccagtta tacaacaata accagataac
     4021 gcaattaaaa ttaatccagc taaaaaagca tttaagcgat acttgaaaaa tttcaaataa
     4081 tttgtctttt gtttcatgat ttttcggttt taaatgattt tgatttatga gttgctgcac
     4141 tatcgctcat tgaacaattt taattctcgt ctaattcagg agtggaaatt atttcatgta
     4201 ttacgttatc gttattaata acacaaaagt aatgtgttta ttataatttt gaaaatgttt
     4261 ttatcgcctc tctaataggt gttccgcctt cgacttcttt tatgagtttt cttggaaatt
     4321 tttgtcggac ttggtaatgg tattaacgtc tactggcgat atcatccttt tcgcttcatt
     4381 ctgcaaaaaa tcaactatat ttgcgatgtt ggtgtgggtt acaaaaagaa aaaaccgctt
     4441 tgtgagcagg ttgaaaagaa ctgtatgcat ttttcatatt caagtagcac ataatacaat
     4501 aaaatactcc cctactctat tcagaagata gtttagggga gtattctatt tacgatgcaa
     4561 agtctcagga aacagtctgg tcgtcgacct gtgcatcggg agcgttggtt tttaccgatt
     4621 taatgccgtt ttccctcgaa gcttcggtct cgtacatttc actgttgcca ataacctgtc
     4681 cgttggaagc tttcaggtta aaataaaatt taccgttttt tgccacgttt ttttcaaaac
     4741 gggcatcttc ttgcgaattc ttctttacag attccacgcc gttcaagcac gcggttttgg
     4801 tagagtaacc ctcactcgat aagatcacct gtccgtttcc ggccttaaga ttgaactgaa
     4861 actcaccact ttttctggtg gtaataacaa attttcccat agtttttttg ttttagttag
     4921 atataaatac cttttctaaa cgatttgcga gtagctttgt tcaacgttat taacaaaaaa
     4981 atgcctcact ttcagtcagt gaggcatttt atacaaaagt ttttttattt ttaattaaaa
     5041 atatatctta atccaagttg tagtctccaa gtctgagagt ttacgtcgtt gtatcttgta
     5101 taaatttccg atactttctc agcattaaag aacgagaaat aaggcacttt gtcatcagca
     5161 ttaccggcta ctccatcttt gtttacacct tcaaatttca gaatttgtcc cctatttgct
     5221 gcaggtatca tttgcttatt aactccccaa gtgctgttca gcaagttggc aaagttcata
     5281 atgtctaaac ttacttgcaa cgtatttgtc gttttacctg ttctcacaat gaagttttga
     5341 gcgaaatgca gatcgaaacg atgaaccata ggagcataag cgccataagc atctgcatat
     5401 tttcctttat ttttgcttaa ataagaatct ttttctacaa aatcccaaaa acggtcagca
     5461 tcctcagctg ttttccattg aatttcatct tttgttgcag gaatataaat caaatcctga
     5521 ttgatagcat caccgttcat atcgttagag tatgtatagc tgtagcgata tggcgaatat
     5581 gcctgataga aaacaccaaa ttcagatgaa gtccatccac ccaggtaatt gatcctgtag
     5641 ttcaaagaag caataatacg atgaggagaa acgtattgtg accattgtgc accgttaaag
     5701 tttggcccgt ttacggtagg taaaccctgc catgcagaag tagcgtttga gcccggcata
     5761 ccacttactt cgtaagaagc tgtatgggta taagctgcca ttaaattcaa atttctgaca
     5821 ggttctgcat tgatagtaat gtttccagta tacccatacc cttcacttgt gttagtcagc
     5881 acacctagca cacccacatt ttttttgtgg tctgcatcgt agcttgcatg gtttctataa
     5941 tagttatagt ttgaagggta aataaaccgt ttgtccggac cttcaaattg agtataccca
     6001 ttagcttgag tttgatcttt gatgttataa ttaagtaagc ggatatcgtt gatgtatttg
     6061 gtaaacattc cttcaacagt taccgataac ggaaatgaaa ccggtatttg ataatctaca
     6121 gccaaagaac ttttccagac ctgaggcatt ttaaaattgt tatccactcc agccatgtta
     6181 ctttgcagtg tgccttcttc ttgggtaata gtctttttta aacccaattt ttcaatcatt
     6241 tggtttacgt ccgtcattaa cttaccgtcc acatacattt gatccagtaa aggattacgg
     6301 cccgttgcag caccggtgtc tttgtcataa gtcgtataaa tctgattgct tgcaccttga
     6361 atcataccgg aattttgggg catgttggtg aagaacacca gaggaagacg gcccgtaaaa
     6421 agcccggtac caccacgtac gacaagtgct ttgtctttaa ggacatccca ggtgaaacct
     6481 acacggggag agaattggaa cttagcatca ggccattttc cggtatccac tttcttacca
     6541 ccgaaatcaa gcgaataaat agcgttgtta cgcaacatat cgtttaggaa gaatgtattg
     6601 tccatacgga ttccgtaagt caacttcaaa ttatcaagtg cgttccactc atcttgaata
     6661 tacgcggcca gctgacctaa cgtcacttta ccggcagggt tcaattctcc gtcgtacccg
     6721 taggaaaggg caaatgcttc gggagcagca ttatttttga aatcggagaa acttttgaaa
     6781 cggtagtatc ccgttgcgtt acgcatataa gaatttaaag cggactgatg ttcgtagctt
     6841 aacccagcag tcaacttgtg cgatgtcagg taataggtaa agttgtctgt aatactaaat
     6901 acattattct ttacaccatt gttccaggta aacaactcgt aaccagcact catatatggt
     6961 ttaaagtttt tgttgtttgt tgggtcttca ccggcagcaa ccacatcatc accatccatt
     7021 atatcaaaga acgggaacgg agaagattta ctgcctcgct gatcattgat atcggtatac
     7081 gtcagtaaca attggttact tatagcgtct gacaaccttg aatttaactc agtagtaaac
     7141 gatttaacga tacttccctg tgaataggta gagttcataa aagccattga attaacacca
     7201 atcctgtcgg ttccatttaa acggtagccg gtatcgcttg agttaccgtt ggtcggaacc
     7261 cagtcgtcat ttttggtata attgtaacgc aagctcagtt tatgagtgga gttaatgttc
     7321 cagtcaatac gtcccagaaa tttaaggttg gaaacaccac ctggaaaatc ggttgatgat
     7381 cctgtatcgt acccataacg ttcttttaaa agacgagaaa attcatccag atcagactgg
     7441 cgtgcatggg agataaattc atcacctttg gcaactccat ttgaagaacc cctccatttt
     7501 gtgatttgtt gagggctttt ttctgtttcg aagttggcaa agaaaaacag tttatctttg
     7561 attatcggac cacctaatgt cgctccgtaa attgtttttc cttctttcgg tcgatcacct
     7621 tccggcgtaa aatcaccaat tgagtttcca cgcatgtttt gattgtaaaa ataggtatat
     7681 gcgctacctt tgtaagtatt tgttcctgat ttggtaatag cgtttacacc gccaccaata
     7741 aagttagatt gacgaacatc aaagggggca attacaacct gcatttcctc aatagcatcc
     7801 agtgaaattg ggtttccacc accaggtaat ctgtcagaca aaccgaagtt gttgttcaaa
     7861 cgtgcgccgt ctacggtaaa attactgtta cgtccatcac gacctgaaaa atccatccct
     7921 gaagatgcat acggagaaag tcgggcaatg tcttgaatgt ttctactggt agtaggcatc
     7981 agcgtaattt gatccgtatt gatattggtt gtggcacctg ttttctctgt tgtaaattta
     8041 gttctttgtg ctgtaacgag tatttcatta agaacttctg aactttcaga catttcaacg
     8101 ttgaccggat aagtttctcc aagctgcaat tgaattcctt ccaattctat tgtattcata
     8161 cccacataag aaacttccac tttatacggt ccacccactc gcataccgtt taaactaaaa
     8221 cgtccatcta cgttagtgac agtcccatac gtggttcccg aaggagtatg tgtggcaacc
     8281 actgtcgccc cgataacagc cccctcattg tcggtgacac ggccgctcat acttgaagtg
     8341 gtcatctggg cgttcactac agtggcgagc agcaagaaaa ataccactgt taaaaacttc
     8401 atcttcttaa tcataaatca atttttaatg tttttggatt atttattaaa aaatctgtac
     8461 aaaattacaa aaatttaatg tgaaaaacat tacgtttttg ttacattttt tagttataca
     8521 atattttaaa tttcaatcga ttgcatcggt aacttttcaa taaatatgta agtaagatag
     8581 tcataaaaca atcaaaaaga aaccacttga ggatttaaaa taacaaacaa atattatcga
     8641 acttaacaca tgttgcggct attttatgtt aaaaataaaa ttccgacaag caaccaacaa
     8701 aaacgcgcga ccatgtggcc gcgcgctttc ctttctttat ttttcaatgt gagagtaaat
     8761 catgcattaa aattaaatta catcattcct cccattcctc ctccacccat aggcatttgc
     8821 ggagcaggat tttcttcttt cttatcggtg atcacgcact cggttgtcag gaacataccc
     8881 gcaatggatg ctgcgttttc cagcgcaaca cgcgctacct tggtaggatc gataacacca
     8941 gtctcgtaca ggttttcata cttatcggta cgtgcgttgt agccgaagtc agctttacct
     9001 tcgcgaactc tctgtacgat aaccgcacct tcttttccag agttggcaac aatctgacga
     9061 aggggttctt ctatagcacg ttttacgatt tcgataccgg tggtttcgtc ttcgttttca
     9121 cctttaaagc cctccagcgc ttcgctggca cgaatataag cgacacctcc accaggaacg
     9181 gttccttctt caacagcagc gcgtgttgcc gaaagggcgt cgtttacacg gtctttcttt
     9241 tctttcattt ctacttccga aggagcaccc acataaagca cagcaacacc accggccagt
     9301 ttagccaaac gttcctgtaa tttttcgcgg tcataatcgg aagtcgtttt ttcgatttgt
     9361 gaacggattt gtcctattct gttggagatg gcatctttgt caccctcgcc gttaacaata
     9421 gtagttgtat ccttgtcgat agtgattttt tcggcacttc cgagcatatc gagtgtagcg
     9481 ttttcaaggg taagtccttt ttcgtctgag atcacggttc cacctgtcaa aatggcaata
     9541 tcttccaaca tttccttacg acggtctcca aagcccgggg cttttacagc agcaatcttc
     9601 aacgatccac gcagacggtt tacaaccaac gttgtcaatg cttccgaatc gatatcttcg
     9661 gcaatgatca acatcggttt gccggtttga attccttttt cgagaatggg aagaagatcc
     9721 ttgatggctg aaatcttttt atcgtgtacc aataccaacg gattttcgaa ctgaacttcc
     9781 atattttcag tatcagttac aaaataagga gatatatatc cgcgatcgaa ctgcatacct
     9841 tctactacat ctacgtacgt atccgtacct ttggattctt caaccgtgat cacaccttct
     9901 ttactcactt tttgcatagc ttccgcaata agcttaccga tagcctcatc accattggca
     9961 gaaattttag ctacgttttc gatcttctta aggtcatcac caatttcaac ggcctgttcc
    10021 ctcaggtttt caaccacctt ggcaacagcc ttatcgatac cgcgtttcag gtccatcgga
    10081 ttagcacctg cagtaacgtt cttcaagcca acaccaatga tggattgtgc caaaacagtg
    10141 gccgttgttg taccatcgcc cgcatcatca ttggtcttgg aagctacttc ctttactaac
    10201 tgagctccca tgttttggaa agagtcttcc aactctattt cttttgcgac cgtaacaccg
    10261 tctttggtaa tttgcggtgc accgtatttt ttgtcgagta tcacgttacg ccctttgggc
    10321 cctaatgtta cttttactgc atctgccaat gaatccacgc ctttcttaag caagtcgcgt
    10381 gcttccattt cgaatttaat ttcttttgcc atatttatta tagattttta gatgtttaga
    10441 tttttgactt cagaccgtta cagtcttgag tccattactc attactacct tttaagaaag
    10501 gattgccaga atgtccgatt gacgcataat caaatacttt tcaccatcaa gttcaatctc
    10561 cgtaccggcg tacttgccat acaagacgtt gtcaccggct ttcacgacca tctcttcgtc
    10621 ttttgttcca tttcctgttg aaataacttc acctttcaac ggtttttctt ttgctgtgtc
    10681 tggaatgatg attccaccta ctgttttttc ttctgcagct gccggtttta ccagcaccct
    10741 gtctgctaat ggtttaatac tcatattgat acaaaattta aataaattat atgatgttag
    10801 ttctaataat ttgtcgtcag acacaagact ctatgcgaat tctgtgccaa aaaaaatgca
    10861 tgtcaatttt ttgataaaat aacttttcaa actgacaaat tttcatattc gctgtctttt
    10921 tagaaaacat ttcatatcgt aaaattacat tcttttacga ttgtcatctc gggttaaaac
    10981 cacaaccaat tctacaacct tcaaggatac ctgcagaaaa taattgatat tttgtacctt
    11041 tgtattttaa aaaagaaaat gaaagcattt gtttcctgga gcagcggcaa agattgcatg
    11101 catgcgctgt atcatttttt gaaaaatccc gaaaaccagg cagtttgttt attaaatatg
    11161 tccggcgaag gaaacggtca accgcattct cacggaataa gcagcgctat gattcgcaaa
    11221 caggcagccc ggctcaacat tcctttacta caaaaagcca catcaagagg caattacgaa
    11281 aaaaacatga aagaagccat tgcccgttta aaatcagaag gtgtagatgc tggcgttttt
    11341 ggggatatct acctgcaaga gcatcgtaca tggattgagc gcgtttgtgg cgaagcaggc
    11401 gtctctgccg ttttcccgct ttgggacaaa agcacttccg gccttatgga acaatttatt
    11461 acggaaagat ttaaaaccat cgtcgtatcc gtaagaaaag aaaaattacc taaatcgttt
    11521 ctgggcagat tgatcgacaa cgatttctta accgacatac atacatttcc cgccaccgat
    11581 ccttgtgggg aaaacggaga atatcacaca tttgtctttg acggacctct ctttgcagca
    11641 ccggttcttt ttgagaaagg agatataacc gaagacgata accattggtt tttagacatc
    11701 aagtaaagat aacagatatg agaaaaagat ttttttctgg catgctcctc cttttttacg
    11761 ttgttgtttc ttttgcccaa accccgtata aaagaatagt atcgcttgct ccctctctca
    11821 cgcagagcct ttattacctg gatgcccaag acaatcttgt aggctgtacc agctactgca
    11881 tggcggccaa agaagataaa aaagaggttg tggcttctgc agtaaaggcc aatctcgaaa
    11941 aagtcattgc cttgaaggcc gatttagtat tggtttcggg atttactgat cttcaagaca
    12001 ttgaaacgtt gagaaaattt ggaataaaag tagaggtttt ccagtcacct aaatcgtttc
    12061 aagaaatctg cggccagttt attcgtctag gtaaacttat aggaaaagag gaaagagccc
    12121 gccagatagt gctcgaaagc gagcaaaaaa taaaaaacat ctcatccgga ataaaatggg
    12181 gaagaaagcc ccggatgttt tttcaaatag gcgctaatcc tctattctcg gtgttgccca
    12241 atacttttat ggatgattac atgaattttt taggagcgga aaatatagca aaaaccttga
    12301 aacggggttc ggtgagccgt gaatttgtaa tagcccaaaa tcccgactat atttttatag
    12361 tcaccatggg catcgtagga aaagaagaaa aagacacctg gagcaggtac tcccaactga
    12421 cagcatcaag aaaaaaacag atacacatta ttgagtcgga cattgcttgt caaccgactc
    12481 ccattacttt tgtacaaaca atggaagtct tggaaaaaat aataaacaaa taaaacttca
    12541 gactgtgaat aacttaggac ttatacatat ttataccggt gacggaaaag gaaaaacaac
    12601 ggcatcggta ggattggcca tacgtgcatt gggaagcgga ttaaaggtgg tttactgctc
    12661 ttttcacaaa aagcctgaaa aatacggata taccgaaatt gatggcctga aaaagcttgg
    12721 cgctcaggta ttcacctttg caaaaggaca tccgcacctg gataaaagta ttgacgaaaa
    12781 cgcaatcaag cgcgaggttt ccgaagcaat cgacaccctt aaccggatgc tgaaaaacga
    12841 tcagacagac atgcttattc tcgacgaaat acttatctcc gtcagggata attacctgga
    12901 agaaaatacc ttaattgatt ttataaaaaa caagcctcct catacggaac tcatcctaac
    12961 gggaagagga gccactccga atgttatgga tctggccgat tacgtgagtt tcgtccggaa
    13021 aataaaacat ccctacgata aaggcgtttt tagt
//
LOCUS       DAOSDR010000027        12816 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrimonas sp. isolate AMR_MDS_1826
            NODE_1892_length_12816_cov_4.658021, whole genome shotgun sequence.
ACCESSION   DAOSDR010000027 DAOSDR010000000
VERSION     DAOSDR010000027.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863355
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrimonas sp. (wastewater metagenome)
  ORGANISM  Petrimonas sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Dysgonomonadaceae; Petrimonas.
REFERENCE   1  (bases 1 to 12816)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12816)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:10:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,720
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 35
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12816
                     /organism="Petrimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1892_length_12816_cov_4.658021"
                     /isolate="AMR_MDS_1826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2023866"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            369..1694
                     /locus_tag="PLI30_02005"
     CDS             369..1694
                     /locus_tag="PLI30_02005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019538702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine 2,3-aminomutase"
                     /protein_id="HOI78357.1"
                     /translation="MNYKSYTRHNFTELPQISNLSSEQIEAIRVVGNVLPFKSNNYVV
                     EQLIDWNNIPDDPIFTLTFPRKEMLSKQHYDKVTNLLQSGADPRVVKNEINKIRLKLN
                     PNPAGQESNIPLFEGEKLHGIQHKYRETVLFFPSQGQTCHAYCTFCFRWPQFTGMSEL
                     KFAMKEAALLHKYLLKHPKVSDVLFTGGDPLTANARILSAYIEPLLSKEFSHIQNIRV
                     GSKSLAYWPYRFLTDKDADDLLFLFEKVNRAGKHLAFQAHFNHPDELMTDAVRRAIER
                     IRNTGTQIRTQSPLLRNINDNPETWSKMWKEQVRLGLVPYYMFVARDTGSKAFFEVPL
                     ARAWNIFRKAYTNVSGIARTVRGPSMSCSPGKVQVLGVAEVKGEKVFVLRFLQCRNPH
                     LVDVPFFAKYSASVTWFDDLKPAFGEKEFFFEEEKLPGKGDRDSTFLWE"
     gene            complement(1703..2620)
                     /locus_tag="PLI30_02010"
     CDS             complement(1703..2620)
                     /locus_tag="PLI30_02010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI78358.1"
                     /translation="MKISTYVASILWLICSLSTAQENKNQGNKNLPLEQLTGYSQTYY
                     FSPGCKERADKIAAFIENAVSYFRKEIAFTPKTTLFILAPQHWEQYARFPVYGMPHNL
                     DYYRLAVASEDNPFWKSFLPSLEQLPPALVERIQNAYRQTDGSYSMQPFFDLLALHEM
                     GHSYHAQAGLKMHRKWQSELFVNIMLHSFVAEKQPELLPALETFPEMVIGAGSSEYRY
                     TSLVDFENNYDDAGKGMTSKNYGWYQSNFHHAAKQIYNAGGSRVVKELWIALKEHQQE
                     MKDNELVDMLRKEVHPSVADVYLKWNQKQ"
     gene            complement(2702..3523)
                     /locus_tag="PLI30_02015"
     CDS             complement(2702..3523)
                     /locus_tag="PLI30_02015"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HOI78359.1"
                     /translation="MKTFIPHTALQEVVLNISTVEAHLPIGTRSAVSPYPPTPYQSLI
                     FYCRNPISMNKNREVEFTQQPLSVLLGPQVSRVNIKVHNRLKAIRVDFLPGAMFRILK
                     VPMYELLDEGFNAGDLFGSKMKILTEQLSEITDLEVGKRLVENFLLERLHSINKQQPF
                     DKALRALYINDGNLSITKTASLSCLSIKQFERKCKERIGMNPKSYARILKFSKAYRLH
                     EAFPEMDWISIAYKSGYYDQMHLIKDFKIFAGANPSEIEKQLRNTAIKMQKDLNF"
     gene            3912..4676
                     /locus_tag="PLI30_02020"
     CDS             3912..4676
                     /locus_tag="PLI30_02020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008124519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOI78360.1"
                     /translation="MGKIIALANQKGGVGKTTTTINLAASLAALEKNVLVVDADPQAN
                     ASSGLGIDIRTLKYTIYEGLIGKCKPADAVMKTPFENIDILPSHINLVGAELEMVQME
                     NRERRLRDLLNPLRATYDYILIDCSPSLGIITVNALTASDSVIIPVQCEYFALEGLSK
                     LLNTIKIIKSKLNTSLEIEGFVLTMYDSRLRLHNQIYEEVKSHFKELVFNTVIQRNIT
                     LSESQSHAKPALMYDAGSRGAINHMQLAQELIEKNV"
     gene            4802..5704
                     /locus_tag="PLI30_02025"
     CDS             4802..5704
                     /locus_tag="PLI30_02025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005784848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HOI78361.1"
                     /translation="MAKKNALGRGLDALITFSEVESQGSTSISEVELSKMFPNPDQPR
                     RVFDEESLEELATSIRKIGLIQPVTLRHEGDDSYQIIAGERRYRASLMAGLTSIPAYI
                     KAADDDELMEMALVENIQREDLNSIEIALAYQNLIDSLSLTQEQLSDRVGKKRATITN
                     YLRLLKLPAEVQMGVKDKKIDMGHARALVTMNDPVAQLSLYNLILEDGLSVRSVEKIV
                     REYAEGKPLNEKSGKKNGKNRENRSGSSFVDDFDALKQHLSNYFKTDVQFNCNKNGKG
                     KITIPFSSAKDLERIIGMFDTMKK"
     gene            5710..6351
                     /locus_tag="PLI30_02030"
     CDS             5710..6351
                     /locus_tag="PLI30_02030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006798793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5683 domain-containing protein"
                     /protein_id="HOI78362.1"
                     /translation="MLKYRNIVCLIFISAAFQLTAQQNTVPQHPDSIKVVSLPDTIIG
                     EKTFKPDPKKAVIYSAIFPGLGQIYNRKYWKLPILYGGFVGLSYAISWNNSHYQDYFD
                     AQRALLDDDPTNDHVWGNMLPYGMDPETADKNWFSGVLKDRKNYFRYYRDFSIILTVA
                     LYGLGIVDAYVDAQLFEFDVSPDLSMRVEPVIFKKADSNYLADSYGFRCSFSF"
     gene            6397..8055
                     /locus_tag="PLI30_02035"
     CDS             6397..8055
                     /locus_tag="PLI30_02035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019538698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HOI78363.1"
                     /translation="MKKFFLILFSCGIISTTIVFAQENLHPNKDNIQDNSIVAPESLN
                     EHLATLLHEWQIDFSKSDNMCRQGANVVFHDSVYTQCLYDMPTEMELSYNQVVRQYID
                     MYANRRRDMVGYMLTIGKYYFPMFEEALDKYGLPLELKYLPVIESALNPVAVSRVGAT
                     GLWQFMLRTGQGYGLQINSLVDERRDPHKSTEAAARYLKDLYAIYGDWNLVIAAYNCG
                     PGNVNKAIARSGGKRDYWQIYYNLPRETRGYVPAFIAANYIMNFYDKHNICPIESFQE
                     MIALDTVHVENQVHFNQIAEVLDIPLSDIRRWNPQFKKDRVPGNYQRYALVLPINKMY
                     AFLEKKDEIMNFRRDDYITHRENTDGYLASSGSSVSGNGENVYYRVRRGDNLSKIASR
                     NGVTVSQLRSWNNLRSTRISAGKNLIVRKKAVDPAVLETDAATMAQQPTNPETATKTV
                     NAYYRVRNGDNLGKIAQRNRVTVAQLKSWNGLRSDRLSVGKQLIVNKKTVTVEEPQPV
                     QQTAETTTSAKDGSGIISNYLKQQLEESTTDETEVVTGASMEAE"
     gene            8104..9060
                     /gene="tal"
                     /locus_tag="PLI30_02040"
     CDS             8104..9060
                     /gene="tal"
                     /locus_tag="PLI30_02040"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019540923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004801 - transaldolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HOI78364.1"
                     /translation="MNLLEQLKTYTQVVADTGDFSSVEIYKPVDATTNPSLIYTASQD
                     TRYRHLIDDAIDFARKSSSDRQVQLPKAMDKLAVNFGLEILKIVPGRASTEVDARLSF
                     DTDATVVKAREIIGLYESAGTSRERVLIKIASTWEGIKAAEIVEKEGIHCNLTLLFSK
                     VQAVACAEAGVRLISPFVGRILDWHKKNLGVADIPAVEDPGVISLTGIFNYYKKFGYP
                     TQVMGASFRNIDEICELAGCDLLTISPTLLRELEATEGILTKKLDADQAREMAIEKIT
                     VDEKTFRWMMNEDAMATEKLAEGIRNFTRDLIKLEKQMEKLL"
     gene            complement(9116..9937)
                     /gene="tsf"
                     /locus_tag="PLI30_02045"
     CDS             complement(9116..9937)
                     /gene="tsf"
                     /locus_tag="PLI30_02045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004584905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HOI78365.1"
                     /translation="MAVTMAEIQHLRKMTGAGMMDCKNALNEANGNFDKAVEIIRKKG
                     QAVAAKREDREASEGCVLAKAQGNYAAVVALQCETDFVAKNEEFIALTQLILDAAIDH
                     KPETLEDLLNIQLPNGTVSQLIMDKIGATGEKMELGYFEHISAPSVVSYIHMGNKLAT
                     LVGFNKEEVAEDIAKDVAMQVAAMNPISVTPDTIPAEVKEKELEIAREKAREAGKPEN
                     LLDRIAEGALQKFYKESTLLQQEYVKDNKLTIDQYLKQNNKDLTVTVFKRVSLNA"
     gene            complement(10085..10945)
                     /gene="rpsB"
                     /locus_tag="PLI30_02050"
     CDS             complement(10085..10945)
                     /gene="rpsB"
                     /locus_tag="PLI30_02050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007655339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HOI78366.1"
                     /translation="MAKLEFDQLLEAGAHFGHLKRKWNPAMSPYIFMERNGIHIIDLY
                     KTIAKTEEAAAALKQIAKAGKKILFVATKKQAKPVIEEKAQSINMPYVIERWPGGMLT
                     NFPTIRKAVKKMSNIDKMIKDGTFDTLSKREKLQITRQRAKLEKMLGSIQDLTRLPSA
                     LFVVDVMKEQIAVKEANKLGIPVFGIVDTNSDPNDIDFVIPANDDAAKAVDMILGYLC
                     EAIKEGLDERKIEKADASAAEDQEEEGAVPRRERKSKVAKRERVKKEDKDAINASVVS
                     KYAKDADVEE"
     gene            complement(11129..11515)
                     /gene="rpsI"
                     /locus_tag="PLI30_02055"
     CDS             complement(11129..11515)
                     /gene="rpsI"
                     /locus_tag="PLI30_02055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004584903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HOI78367.1"
                     /translation="MDVINSIGRRKAAVARVFMSEGSGKIVINKRELENYFPSSILQF
                     IVKQPLNKLNVTEKYDIKINLQGGGYKGQSEAARLAIARALVKLNPEDKPALRAEGFM
                     TRDPRVVERKKPGRPGARKRFQFSKR"
     gene            complement(11520..11975)
                     /gene="rplM"
                     /locus_tag="PLI30_02060"
     CDS             complement(11520..11975)
                     /gene="rplM"
                     /locus_tag="PLI30_02060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005859486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HOI78368.1"
                     /translation="MNTLSYKTISVNKETAQKEWVLVDASGQSLGRISSKVAKLLRGK
                     YKPSFTPHVDCGDNVIIVNADKVELTGNKWTDRVYYRHSGYPGGQKVSTPADLMKKSP
                     DRLFRKVVKGMLPKNKLGDAILNNLYVYAGNEHPHQAQKPKQIDINTLK"
     gene            complement(12198..12656)
                     /locus_tag="PLI30_02065"
     CDS             complement(12198..12656)
                     /locus_tag="PLI30_02065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI78369.1"
                     /translation="MKKVSVLTVLFLVAFIGAIFSQHNFALNLGYDHMQAHTGFLGVE
                     YRVDHNLQRHTSHGPFTVGIGTYLYGENGKFSPAPEIHLNKTWKHFIINEISVSTKNI
                     KPSVGITFFNLTRLQFGYSFPIQQSDLRGFSVGIHVLIGRSPFYDEIRVF"
BASE COUNT         3572 a         2605 c         2816 g         3823 t
ORIGIN      
        1 gatttacttg tacggttgca acgtaatagg gttgattttt acggctataa cctaaaatcc
       61 gtaaaaaccg gaagggttag tggcatcgtc ggactaaatc cataacgatg tatctgggaa
      121 cgatatgtgt tagtaatact ttcattggat gtgcaaaagt aacacttata tttaataaaa
      181 acgagaaaac gacaggaaat ttcatctaaa aaaataaaat agaaattctt gccaaattat
      241 taaaaaaaag cgtaaagagt gtttacaaat gatgggttgt tttttgattt ccctattttt
      301 gtaaaatgat gtgagtgatt tttatgtcat ttgcagtgag ttacacatcg gaaaaatcaa
      361 gaacaaaaat gaattacaag agttataccc gacacaattt tacagagtta ccacagatat
      421 ccaacctatc atccgaacaa attgaagcca ttcgggtggt aggaaatgtt cttcccttca
      481 agtcgaacaa ctatgtagta gagcaactga tcgattggaa caatataccc gacgatccca
      541 tttttacgct tactttcccg agaaaagaga tgttgtcgaa acaacattac gataaagtga
      601 caaatttatt gcaatcagga gcagatccgc gggttgttaa aaatgagata aacaaaatca
      661 gactgaagtt aaatccgaat ccggcaggac aggagagcaa cattcctctg tttgaagggg
      721 agaagctgca cggaatccag cataaatacc gggaaacagt gttgtttttc cctagccagg
      781 gacaaacttg tcacgcttat tgcacgtttt gttttcgctg gccgcaattt acaggaatga
      841 gcgaactaaa atttgcgatg aaggaagccg ctctgcttca caaatacctg cttaaacacc
      901 ctaaagtttc tgatgtgctg tttaccgggg gagatccttt aacggcaaat gcaagaattt
      961 tatcggctta catagaaccg cttttatcga aagagtttag ccatatacaa aacatacggg
     1021 ttggttctaa atcacttgct tactggccct accggttttt gacggataaa gatgccgacg
     1081 accttctttt tttattcgaa aaagtaaata gagcaggcaa gcacctggca tttcaggcac
     1141 acttcaatca tcccgatgaa ttaatgacag atgctgttag gcgggcgatt gagcggatac
     1201 ggaataccgg aacgcagatc cggactcaat caccgttgtt acgaaacatc aatgataatc
     1261 cggaaacctg gtcgaagatg tggaaagagc aggtccgttt ggggcttgtc ccgtattata
     1321 tgtttgttgc ccgtgatacc ggctcgaaag cgttttttga agttcctctg gcaagggcat
     1381 ggaatatctt caggaaggct tatacgaacg taagcggaat tgcccgaacg gttaggggcc
     1441 caagcatgag ttgcagtccc ggcaaggtcc aagtcctggg agtagctgaa gtgaaagggg
     1501 agaaagtatt tgttctccgg tttctacagt gccgcaatcc ccacttggtg gatgtccctt
     1561 tttttgcaaa atacagcgct tcagttacgt ggtttgatga tcttaaaccg gctttcggtg
     1621 agaaagaatt tttctttgag gaggaaaaat taccgggaaa aggagacaga gacagtacat
     1681 ttttgtggga atgattaggc tttcactgct tctggttcca cttcaaataa acatccgcca
     1741 ccgatggatg cacttctttc cttaacatgt cgaccaattc attatctttc atctcctgtt
     1801 gatgttcttt taacgctatc caaagttctt taactactct gctgcctccg gcattgtaaa
     1861 tttgcttcgc agcgtggtga aaattacttt gataccatcc gtagtttttg ctagtcatgc
     1921 ctttccccgc gtcatcgtaa ttgttttcaa agtcgaccag ggaggtgtag cggtattccg
     1981 atgaacccgc accgataacc atctccggaa aagtttccaa agcaggcaga agctcaggtt
     2041 gtttttccgc gacaaaagag tgtagcataa tattgacaaa caattcactc tgccattttc
     2101 tatgcatttt taacccggct tgggcgtgat aggaatggcc catctcgtgt aaggccaaga
     2161 ggtcgaaaaa aggttgcatg ctgtagctcc catcggtttg gcgataggca ttctggatgc
     2221 gctcaaccaa tgctggaggc aattgttcca atgatggcaa gaaacttttc cagaacggat
     2281 tatcttccga tgccacagcc aaccggtaat aatccaagtt gtgtggcatt ccataaacag
     2341 gaaatctggc gtattgttcc cagtgttgcg gggcgagtat gaaaagtgtt gtctttggcg
     2401 tgaaagcgat ctctttcctg aaataagaaa cagcgttttc tataaaggcg gctattttgt
     2461 ctgccctttc cttacagccg ggactgaaat aatatgtttg agaatagcct gttaattgct
     2521 ccaggggtaa gttcttgttt ccctggtttt tgttttcctg agcagttgat aaactgcaaa
     2581 ttaaccacag tatggatgcg acataagttg aaattttcat ttaattacag tttaaagggg
     2641 atgttaattt caacaaatat agatgctgca ccaaccgtgt gcatagtaaa aaatcgacac
     2701 tttaaaagtt caggtctttt tgcattttaa tagcggtatt ccggagttgt ttttcaattt
     2761 ctgacggatt tgccccggca aaaattttga aatcttttat caggtgcatc tgatcgtagt
     2821 agcccgattt gtacgcgata cttatccaat ccatttcggg aaatgcttcg tgtagcctgt
     2881 aagccttcga aaatttcagg atccgtgcat aactcttggg attcattccg attctttctt
     2941 tacactttct ttcgaattgt ttgatgctca gacaagatag cgacgctgtt tttgtgatgc
     3001 ttaaattacc gtcgtttatg tacagcgcac gtaaggcttt atcgaaaggt tgttgtttat
     3061 taatactgtg caaccgttcc aacagaaagt tttccacaag ccttttccca acctccaagt
     3121 cagtaatttc agatagttgt tcagttaaga ttttcatttt tgatccgaaa agatcccctg
     3181 cattaaaacc ttcgtcaagt agttcataca tcggaacttt tagtatccgg aacattgcac
     3241 cgggtagaaa atccacgcga atggctttta atcggttgtg cactttgata tttacacgtg
     3301 aaacttgtgg gcctaacaag acactcaaag gttgttgggt gaattcaacc tctctgtttt
     3361 tattcattga aatcggattt ctgcaataaa aaatcaatga ttggtaggga gtgggagggt
     3421 agggcgatac cgcactccgg gttccaatgg gcagatgagc ttctaccgtg gaaatattta
     3481 atacaacttc ttgtaaggcc gtatgcggta tgaatgtttt cattttcctt ttttattacc
     3541 gtcgttttgt cttaggtaaa tgatatttac tttccgtata gttatttaaa atgattctat
     3601 attatatctt tgcaaatata ctataagaat agccgaatga tacccaacga gaattttttt
     3661 ttccgaaaac agtgcttgtt gtttttttga tgatcaatca actttagcat gtattacgat
     3721 ggcaactatt tcccggggcc gggtaatttc ggaaagcatg cgtcgaccat aatcaggaag
     3781 atggatttct aacaaggaga attattcccg gagtactcat tttttcaaca aaaaacagaa
     3841 cgttttattt aattaacata gaattatggg aattattgct tattttgctt tttcaattgg
     3901 gagattatta aatgggcaaa attattgcat tggcaaatca gaaaggtggc gtgggaaaaa
     3961 ccactactac tattaatcta gcagcatcgt tggctgcttt ggaaaagaat gtgcttgttg
     4021 tggatgccga tcctcaggcg aatgcttcgt cgggattggg tattgatatt cggacgttga
     4081 aatacacgat ttacgagggg cttatcggca aatgtaagcc agctgacgca gtgatgaaaa
     4141 caccttttga gaatatcgac attcttccct cacacattaa cctggtagga gcagagttgg
     4201 agatggtcca gatggaaaat cgtgaaaggc gtttgcgcga tctgctgaat ccgctgcggg
     4261 ccacttacga ttatatcctt atcgattgct ccccgtcgct tggtattata actgtgaacg
     4321 ctttgacagc ttcggattcc gttattatcc ccgttcaatg tgaatatttt gccctggaag
     4381 ggttgagtaa gttgctgaac accatcaaga taatcaaatc gaaactgaat acaagcctcg
     4441 agattgaagg ttttgtgctt accatgtacg attcccgcct gcgtttacat aatcaaatat
     4501 acgaagaggt aaagagccac tttaaggaac ttgtctttaa tacggttatt cagcgaaaca
     4561 tcacgctcag tgagtcgcag agccatgcta aaccggcgct aatgtacgat gccggatcac
     4621 ggggagccat taaccatatg cagttggcac aggaattaat cgagaaaaat gtttaacttt
     4681 gtaataagaa gattgattaa agatcgaaaa tagattgatg cggattaact gctctcagct
     4741 tatctccgtt caatctcatt cagtctcgac gaagtttcaa tctccattca atctatagat
     4801 tatggcaaaa aaaaacgcac taggaagagg cttagacgca cttattactt ttagtgaagt
     4861 tgaatcgcaa ggctctacgt ccatcagtga ggtagaactt tcgaaaatgt ttcctaatcc
     4921 cgaccagccc cggcgtgttt tcgatgagga gtctcttgaa gagctggcta catccattcg
     4981 gaaaataggg ctcattcagc cggtaacgtt gcggcatgag ggtgatgact cttaccagat
     5041 cattgccggg gaacgcagat acagagcctc gttgatggcc ggcttgactt ctatccctgc
     5101 gtacataaaa gctgcggatg atgacgagtt gatggagatg gcactggttg aaaatattca
     5161 gcgcgaagac cttaactcca tcgaaatagc actggcttat caaaacctta ttgattcact
     5221 ttcgcttacc caggaacaat tgagcgatcg agtggggaaa aaacgggcaa ccattaccaa
     5281 ctacctgaga ttgctgaaac tccctgccga agtacaaatg ggagtgaagg acaaaaaaat
     5341 agatatgggc catgcacgag cattggttac tatgaacgat ccggtggcac agctctccct
     5401 atacaacctt atcctggaag atggattatc ggtcagaagc gtggagaaaa ttgtccgtga
     5461 gtatgctgaa ggaaaaccgt tgaatgaaaa atctggcaaa aagaatggta agaaccggga
     5521 gaatcgttcg ggaagttcct ttgtcgatga ttttgatgcg ttgaagcaac acctttccaa
     5581 ctattttaaa accgatgttc aattcaactg caacaaaaac ggcaagggga aaattacgat
     5641 ccccttttcc tctgctaaag atttggaacg gattatcggt atgtttgata caatgaagaa
     5701 gtaacgttta tgctgaaata ccggaacatt gtttgtttaa tattcatcag tgccgccttt
     5761 cagttaacag cgcagcaaaa cacggttcca cagcaccctg actccattaa agttgtttct
     5821 ctcccagaca ccatcatagg ggaaaaaacg ttcaaacccg atccaaagaa agccgtgata
     5881 tattcggcca tttttccggg attggggcag atatacaaca gaaagtactg gaaattgccc
     5941 atactttacg gtgggtttgt agggctttca tacgctatat cctggaataa cagccattat
     6001 caggattatt ttgatgcaca aagggccttg ctggacgatg atccgaccaa tgatcatgtt
     6061 tgggggaaca tgttgcccta cggaatggac ccggaaacag cagacaagaa ttggttttcc
     6121 ggagtgttga aagacagaaa aaactacttc aggtattacc gggatttcag tataatttta
     6181 accgtggcac tttatgggct tgggattgtg gatgcctacg tagatgcaca attatttgaa
     6241 tttgatgtga gccccgatct ttcgatgcgt gtggagccgg taattttcaa aaaggcagat
     6301 tcaaattacc tggcggattc ttacggattt agatgcagtt ttagttttta agattacaac
     6361 cggaagctct ttgcgggttt ccctgaaaaa gagagaatga aaaagttttt tttgatcctg
     6421 ttttcgtgtg gaataattag tacaaccatt gtttttgcac aagaaaattt gcacccaaac
     6481 aaagataata ttcaggataa ctccatcgtt gcacccgaaa gcctgaacga acacctggct
     6541 acgttactgc atgagtggca gatcgatttt tcaaaatcgg ataacatgtg caggcaaggc
     6601 gcaaatgtag tctttcatga tagtgtttat acccaatgtc tttatgatat gcctacggag
     6661 atggaattat cctataacca ggtggtcaga cagtacatcg atatgtacgc caaccgtcga
     6721 cgcgatatgg taggatatat gctaactatc ggtaaatact attttccaat gtttgaagaa
     6781 gctctggata aatacggctt acctctggaa ttaaaatatc tacccgtaat tgagtcggca
     6841 cttaatccgg tggctgtttc gagggtggga gcaacgggct tgtggcagtt tatgctgagg
     6901 acgggacaag gttacggact tcagataaac agccttgttg atgagcgccg tgatccgcat
     6961 aaatcgaccg aggcagccgc acgatacctt aaggatcttt atgcaatcta cggtgactgg
     7021 aacctggtta ttgctgctta caactgcgga ccgggcaatg taaataaagc catagcccga
     7081 agcggaggca agcgcgatta ctggcaaatc tattacaact tgccgcggga gacccgcggt
     7141 tatgtgcctg catttattgc agcgaactat atcatgaatt tttacgataa gcacaatatc
     7201 tgtccgatag agtcatttca ggagatgatc gccctcgata ctgtacacgt ggagaatcag
     7261 gtgcacttta accagatagc cgaggttctg gatattcccc taagtgatat ccggcggtgg
     7321 aatccccaat ttaaaaagga cagggtaccg ggtaactatc aacggtatgc acttgttttg
     7381 cctatcaata agatgtatgc gtttctcgaa aagaaagatg aaataatgaa ttttcggaga
     7441 gatgactata ttacgcatcg cgaaaatacc gatggatatc tggcttcgtc aggatcatcc
     7501 gtctcaggaa acggagaaaa tgtttattat agggtaagaa gaggggacaa cctttcgaag
     7561 atcgcatcta ggaacggagt tactgtctca caactccgct catggaataa cttaaggtct
     7621 acccggattt ctgcaggcaa aaatcttatc gtcaggaaga aagctgttga tcctgccgta
     7681 ctggagacgg atgcagccac gatggcacag caacccacaa atccagaaac tgcaacaaag
     7741 actgtgaatg catactatcg ggtacgcaac ggcgataacc tgggcaagat cgctcagaga
     7801 aatagagtga cggtggcaca gctgaaatcg tggaacggat tacggtccga ccggctttcg
     7861 gtggggaaac agctgattgt aaataagaaa accgttaccg ttgaagaacc acaacctgtt
     7921 caacagactg ctgaaacgac tacctcggct aaggatggtt ccggtattat ttccaattac
     7981 ctgaaacagc agctcgagga aagcacaacc gatgaaacgg aagttgtaac cggtgcttca
     8041 atggaagcgg aataagcgct attgcaccat actgtttatt gttaaattta atttctcagc
     8101 attatgaacc tgcttgagca actaaaaacc tatacccagg ttgttgcgga tacgggtgat
     8161 ttttcatcgg ttgaaattta taaacccgtt gacgctacca ctaacccttc gttaatctat
     8221 actgcttcac aggatacccg gtacagacac ctgattgacg atgctatcga ttttgcccgg
     8281 aaatcatcgt ccgacaggca ggtacaactt cctaaagcca tggataaatt agccgtgaac
     8341 tttgggttgg aaattttaaa gatagttccc gggagagctt caacggaagt cgatgcgcgt
     8401 ctatctttcg atacggatgc aacagtagtc aaggcccgtg aaattatcgg attgtacgaa
     8461 tctgcaggaa cttcccgtga acgggtactg ataaagattg cttctacgtg ggaaggaata
     8521 aaggcggcgg aaatcgttga aaaagaagga atacattgta acctcaccct cctcttttcc
     8581 aaggtacaag ctgttgcttg tgccgaagcc ggtgtacgat taatttcacc gttcgtggga
     8641 aggattctcg attggcataa aaaaaacctc ggtgtagcag acataccggc tgtggaggat
     8701 cccggagtga tttcgttgac tgggatattt aattattaca agaagtttgg ttatccaacc
     8761 caggtaatgg gtgcgagctt ccgtaatatt gacgagatct gcgagctggc tggatgtgat
     8821 ctgctcacta tttccccgac gttgctcagg gagctcgaag ccacagaggg tatactgacg
     8881 aaaaaactgg atgcagatca ggcccgtgaa atggctatcg aaaaaattac cgttgacgaa
     8941 aaaaccttcc ggtggatgat gaacgaagat gcaatggcta ccgaaaaatt ggccgaaggc
     9001 atccgcaact tcaccagaga tctgattaaa ctggagaagc agatggaaaa gctgttgtaa
     9061 caaaaaaagt aagggcggaa cttaaagtcc cgcccttagc ccttatctct cttcgttatg
     9121 cgttcaacga tacccgtttg aaaacggtta cggtaaggtc tttgttgttt tgtttgagat
     9181 actgatcaat ggtcagtttg ttatctttga cgtattcctg ttgcagtagt gttgattctt
     9241 tgtagaactt ctgcaaagcg ccttcagcaa ttctgtctaa caggttctcc ggtttcccgg
     9301 cttcgcgtgc tttttcgcgt gcaatttcca gttccttctc tttaacttct gccggaatag
     9361 tatcgggtgt taccgaaatt ggattcattg ccgctacctg cattgctacg tcttttgcta
     9421 tgtcttcagc cacttcttcc ttgttgaatc caacaagggt cgccaacttg tttcccatgt
     9481 gaatatagga aaccaccgat ggtgcggaaa tgtgttcaaa ataacccagt tccatttttt
     9541 cgccggtagc gccgatctta tccataatta actgggaaac ggttccgttg ggaagttgga
     9601 tattcagcaa atcctccaac gtctcgggtt tatgatcgat agctgcatcc agtatcagtt
     9661 gagtcaatgc gatgaattct tcatttttag caacaaagtc ggtttcgcat tgcaatgcta
     9721 ctactgcagc atagtttcct tgagcttttg caagcacaca cccttcactt gcttcgcgat
     9781 cttcgcgttt ggcggcaact gcctgccctt ttttgcggat aatctctact gctttgtcaa
     9841 agttgccgtt tgcttcattt aatgcatttt tgcaatccat cattcccgct cctgtcattt
     9901 tacgtaaatg ctggatttct gccattgtaa ctgccatatt tctatgtttt tttaagttgt
     9961 tttcttttta gatttcaaag aagagacctt agaccgcctc gctggtagat ttcagacgtt
    10021 ggaacattga agcatccaag tgtctgttat cttgacatct gatagctggc cgttgttgtc
    10081 cgtattattc ttcaacgtct gcgtctttag cgtatttgct tactacagat gcattgatag
    10141 catctttgtc ttctttctta acgcgctctc tcttagctac ttttgatttt ctttccctgc
    10201 gaggtactgc accttcctct tcttggtctt ctgctgcgct ggcatcggct ttttcaattt
    10261 tacgctcatc caggccttct tttattgctt cacaaagata tccgaggatc atgtctacgg
    10321 cttttgcagc atcatcgttt gcaggaatta cgaaatcaat gtcgttcgga tcagagttgg
    10381 tgtcaacaat accgaatacg ggaatgccaa gtttgtttgc ttctttcaca gcaatctgtt
    10441 ctttcattac gtcaacaacg aaaagagcgg atggtagacg ggtaagatcc tgaatcgagc
    10501 cgagcatttt ttcgagttta gcacgctgac gggtgatttg cagtttttcg cgtttcgaca
    10561 acgtgtcaaa tgttccgtcc ttaatcatct tatcaatgtt actcattttc ttcactgcct
    10621 tgcggatagt gggaaaattg gtaagcatac caccaggcca acgctctata acgtaaggca
    10681 tattgatgct ttgtgccttt tcttcaataa ccggttttgc ctgtttttta gtcgctacga
    10741 agagtatttt tttgccggct ttggcaattt gttttaaagc cgctgcggct tcttcggttt
    10801 ttgcaattgt tttgtaaagg tcgataatgt gaataccgtt gcgctccata aaaatatagg
    10861 gagacattgc aggattccat ttccttttca agtggccgaa gtgtgctcct gcctcaagta
    10921 gttggtcaaa ttctaatttt gccattgttt gtgttgtttt tttattcgtt tacattcttt
    10981 ttcttagtcg aaccgtaagg taaattaccg aagcgattat tcccggtaat gtccttacga
    11041 tttagatact aaacgtcact aagtggaacc ggtttgtgcg gttcttatat atctttccgg
    11101 caaaagaacc ggtgaatata tccaaaaatt aacgttttga gaattggaat ctcttccttg
    11161 ctccgggacg ccctggtttt ttacgttcaa caacgcgggg atcgcgggtc ataaagcctt
    11221 cggctcttaa cgcaggttta tcttcggggt ttaacttcac caatgcacgt gcaatagcta
    11281 gtcttgctgc ttccgattgt cctttgtatc caccgccctg caggttgatc ttgatatcgt
    11341 atttttcagt cacgttgagt ttattcaacg gttgtttcac gatgaactgc agaatggatg
    11401 aaggaaaata attttcaagt tcgcgtttgt tgataacgat cttgcctgaa ccttcactca
    11461 tgaatacacg agctacagcg gctttgcgtc ttccaattga atttattacg tccatcttat
    11521 tatttgagtg tattaatatc tatttgtttg ggtttttgag cttgatgcgg atgttcgttt
    11581 ccagcataca cgtaaagatt gttcagaata gcatcgccga gtttattctt cggcaacatc
    11641 ccttttacca cttttcggaa aagcctgtcg gggctttttt tcataagatc ggcaggagtg
    11701 gatacctttt gaccgccggg atagcccgag tgacggtagt aaaccctgtc tgtccatttg
    11761 ttgcccgtga gttcaacttt gtcggcattc acgataatca cgttatcacc acaatccacg
    11821 tgcggagtaa agcttggttt gtacttgcct ctcaacagtt ttgccacctt agaacttata
    11881 cgtcctaacg attggccgct cgcatcaacc aaaacccatt ctttctgagc cgtttctttg
    11941 tttacggaaa tggttttata acttaaagta ttcacttttt tttgttttta aatcgtttaa
    12001 taaaattaag ttctgatttt cgctccccgt tttgtctggc taagggacta aatggcaaat
    12061 aaaaagctat aaatcaaact tttaagtaat aaattttaat aatcggactg caaagttaca
    12121 ataattcagg taattaacaa agcgttttta gacgaaaata tgcataataa ctctaatttt
    12181 tttgagtctg cgcatgatta gaatacccgt atctcgtcgt aaaagggaga tctcccaata
    12241 agaacatgga ttccgacaga aaatcctctt aaatcagatt gttgtatggg aaagctataa
    12301 ccaaactgta accgggtcag gttaaagaat gtaatcccca cgctgggttt aatatttttg
    12361 gttgatactg atatttcgtt gattatgaaa tgtttccagg ttttattcag gtgtatttcc
    12421 ggcgcagggg agaattttcc gttttctccg tataagtagg tgcctatgcc gacagtaaaa
    12481 ggaccgtgcg aggtgtgtct ctgtaaattg tggtctaccc ggtattccac ccctaaaaat
    12541 ccggtgtgtg cctgcatgtg atcgtaaccc aggtttaaag caaaattatg ttgtgaaaag
    12601 attgcaccaa taaatgcaac taaaaataag acagttaata cggatacttt cttcatagaa
    12661 tcatttttcc tacaaatata aaacataaaa acatttgtgt agttttaaaa attgatttaa
    12721 ctttgcaggc gaaaattaca aaaacaacgg ccccgtagct taactgaata gagcatctga
    12781 ctacggatca gaaggttaca ggtttgaatc ctgtcg
//
LOCUS       DAOSDR010000028        12779 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrimonas sp. isolate AMR_MDS_1826
            NODE_1898_length_12779_cov_4.233810, whole genome shotgun sequence.
ACCESSION   DAOSDR010000028 DAOSDR010000000
VERSION     DAOSDR010000028.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863355
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrimonas sp. (wastewater metagenome)
  ORGANISM  Petrimonas sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Dysgonomonadaceae; Petrimonas.
REFERENCE   1  (bases 1 to 12779)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12779)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:10:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,720
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 35
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12779
                     /organism="Petrimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1898_length_12779_cov_4.233810"
                     /isolate="AMR_MDS_1826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2023866"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1345)
                     /locus_tag="PLI30_02070"
     CDS             complement(<1..1345)
                     /locus_tag="PLI30_02070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006841745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HOI78370.1"
                     /translation="MLVKVFGAAVQGINATLITIEVNCTKGIKFFLVGLPDASVKESH
                     KRIVSAIQVSGFKYPRQQIVINMSPADIRKEGSAYDLPLAIGILATSETIIADKLPDY
                     LIMGELSLDGNILPIKGALPIAIMAKENGFKGFILPTENAKEAAVVEGLEVYGVENIR
                     QVVGFANNELELEKTTADIGQEFLQSQSNFEFDFSDVKGQENVKRALEVAAAGSHNII
                     MVGPPGAGKSMMAKRMPSILPPFTLEEALETTKIHSVAGKLEKGISLMSRRPFRSPHH
                     TISNVAMVGGGADPQPGEISLAHNGVLFLDELPEFNRNVLEVMRQPLENRKITISRAK
                     YSVEYPASFMMVSAMNPCPCGYYNHPDKACVCPGGAVQKYLNRISGPLLDRIDIHIEI
                     VPVAFEKLSDSTPAERSAEISKRVVKARRIQAERFRKTPGIYCNAQMTSRMLLEYA"
     gene            complement(1351..2478)
                     /locus_tag="PLI30_02075"
     CDS             complement(1351..2478)
                     /locus_tag="PLI30_02075"
                     /inference="COORDINATES: protein motif:HMM:NF012787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-like domain-containing protein"
                     /protein_id="HOI78371.1"
                     /translation="MKFVKLSILIIICTLLFSCKNNENGVALKGDISSLENPQVLVSY
                     FAGDTLAIDTIMSDKRGRFTYKNTIDTLTVFSLYFNNQSSSVMVFAHPKDNISIKGDA
                     QLSDLIKVNGNEINNDLTLFKESNKELLQQRTLLLRNLKEEKESNSASNNRILANADE
                     IAKINSFNQELTLNAEEQVKKNPTKISSLILINEFFANSENPKGLSRVLEYMQGDILK
                     SKMGLNLKAYSEKINRSAEGSSMPYFRLVDKSGDTIQSYDYRGKYLLLSFISTTGIDS
                     RETIQSLKQTYRDVNKDSVEFISVYIDSDIYPISYIENDSIPWTVVPEKKSWASDIVE
                     AYNIEFIPNNILISPAGIISDRNVPAIAVENALKNSVKNNR"
     gene            2711..3700
                     /locus_tag="PLI30_02080"
     CDS             2711..3700
                     /locus_tag="PLI30_02080"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005810799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOI78372.1"
                     /translation="MNFSVVIPVYNRPEEVDELLDSLTRQSGKNFEVIIAEDGSSEKC
                     DGIVQKYVSQLPVVYFEKPNSGPGLTRNAGAKKAKYDYLIFFDSDCIIPEHYMEYVSF
                     YLERNFVDAYGGPDNALPSFTTVQKAINYSMTSFFTTGGIRGGKKSMDKFHPRSFNLG
                     VSKKAFEALGGYGKMRFGEDIDFSLRLLKKGFGTALIPEAYVYHKRRSTFRQFFKQVY
                     NSGIARINLHLAHPGSLRLVHFLPSFFVTVMVLSLLLSFFYPVVVLAPALYSLLVLID
                     STARNNSLRVGFYSIAAAWTQLFGYGAGFISAIWKRLILKKGEFNAFEKKFYE"
     gene            complement(3713..4372)
                     /gene="upp"
                     /locus_tag="PLI30_02085"
     CDS             complement(3713..4372)
                     /gene="upp"
                     /locus_tag="PLI30_02085"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019538302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004845 - uracil phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HOI78373.1"
                     /translation="MNVIDFSKNNSLLNSFVREIRDITIQNDRLRFRRNLERIGEIMA
                     YEISKDLSYKTISVTTPLDVAEVSVPDEEIVIGTILRAGLPYHHGVLNYFDCAGNAFV
                     SAYRKYKETGHQSFDIHIEYIASPSIDGKTLILTDPMLATGSSMELTYRALLTKGTPR
                     RIHIASIIASQQAVDYCTQHFPEDKTTIWVAAIDPELNESSYIVPGIGDAGDLAYGEK
                     I"
     gene            4688..6286
                     /gene="pckA"
                     /locus_tag="PLI30_02090"
     CDS             4688..6286
                     /gene="pckA"
                     /locus_tag="PLI30_02090"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014224360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004612 - phosphoenolpyruvate
                     carboxykinase (ATP) activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HOI78374.1"
                     /translation="MANLDLSKYGIVGNFEILHNPTYEELFQAEIDPANEGFEKGVLT
                     STGAVAVDTGKFTGRSPKDKFIVMDDTTRDTLWWDGTINRPTTPEVFDHCKDLVTTQL
                     STAKKLYVVDTFCGTNEDTRMKVRFVMEVAWQAHFVTNMFIRPSHYELANYGEPDFVC
                     LNGSKTSNPNWKEQGLNSEVFTLFDLTRKMQVIGGTWYGGEMKKGIFALMNYYLPLKG
                     IASMHCSANVGTEGDVAIFFGLSGTGKTTLSADPKRYLIGDDEHGWDDHGVFNYEGGC
                     YAKVIDLEKDKEPDIWRAIRRDALLENVTVLPDGTPDYSAAASKTENTRVSYPIYHIS
                     KIVSPSRAGHASKIIYLSADAFGVLPPVSILDEKSAQYHFLSGFTSKLAGTERGITEP
                     VPSFSPAFGEAFLTLHPTMYAKTLIGKAQEHNAKVYLVNTGWNGTGKRISLKNTRAII
                     DAIIDGSIEEAETIQIPILNLVAPAALNNVDTEILDPRNTYADVAEWEAKAKSLAAKY
                     IKNFEQYCDNDDALALVASGPQLD"
     gene            complement(6354..7151)
                     /locus_tag="PLI30_02095"
     CDS             complement(6354..7151)
                     /locus_tag="PLI30_02095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019538299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1080 domain-containing protein"
                     /protein_id="HOI78375.1"
                     /translation="MKQVWSLLGATTAAFILISCSQKKSSDASSGWTPLFNGTDLTGW
                     TVKIKGYDAGDNFGNTFRVEEGMIKVRYEQYDSLRDRFGHLFYNDEFSHYKLRVEYRI
                     VGEQCPGAPGWAYKNSGIMVHGQTPESMEKDQDFPTSIEVQLLASDSLVQRTNMNVCT
                     PGTNIVMDDQLILDHCINSSSENFYGEDWITAEVEVRGNNVISHIINGDTVLQYNRPQ
                     LDERDATYAKLIVMNGGDKMLSKGTISLQSEGHPIDFRKVEIMKLDD"
     gene            complement(7160..8347)
                     /locus_tag="PLI30_02100"
     CDS             complement(7160..8347)
                     /locus_tag="PLI30_02100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylxylan esterase"
                     /protein_id="HOI78376.1"
                     /translation="MKKEILYLLLFTLTTTLFAQNYDETKAGSYVLPELLKAQNGKEI
                     VSAEDWDRIRRPEILKLFEDNVYGQVPKDFDKIEFKVLEQNYKALNGKATYKQVAVDV
                     IRNKKVITVQVHLFIPNKIKKPVPVFLTINHRGLDTMQASPENEFWPAEEIVNSGYAI
                     AGFDVKDVAPDHKTDYVNQILTKLYPEQTLQPNGMRALGAWGWGVSRIIDYFETDKDI
                     DASKVIVVGHSRGGKAALWCGAQDRRVAIAVSNESGNSGAKISRRNFGETVEVITRNF
                     PHWFVPGYAAFANNEGNLPVDQHMLLALMAPRAVYVASAADDSWADPKGQYLALVAAQ
                     PVFSLFGLKTSLPANMPPNNEQVIQLPLGFHNRDGIHNMNLFDWKQFVKFADEYFKNN
                     NKK"
     gene            complement(8395..9372)
                     /locus_tag="PLI30_02105"
     CDS             complement(8395..9372)
                     /locus_tag="PLI30_02105"
                     /inference="COORDINATES: protein motif:HMM:NF028437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5106 domain-containing protein"
                     /protein_id="HOI78377.1"
                     /translation="MNQYSLKSFFVLTAFLMIYACSSSKKAQIDTIGEDSLKREQTIV
                     PDTFVLPYIPEEMTNSDARAVYLVMHYWDRFDFSDEKLTLRPEITEQAFVDYINILSY
                     VPFDRTKESLNYTLRKAEANNTMYTYFGTLFDKYFYGANSPFRNEEFYIPVLQELVNS
                     EILSETDKSRYRFQFEMVMKNRVGETANDFAYTLASGESQRMHSIKSEYLVLMFSNPG
                     CSTCASVTDVLTKSVTLNKAFSMNSPTRTMITVLTVYPDADIDEWRSYLPQMPANWIH
                     SYDKETTITKQKLYDIKAIPTLYLLDKDKKVILKDTSIEAIESFFAKPN"
     gene            9567..11285
                     /locus_tag="PLI30_02110"
     CDS             9567..11285
                     /locus_tag="PLI30_02110"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006841575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HOI78378.1"
                     /translation="MDNYVVSARKYRPVTFRSVVGQEALTTTLKNAITGNKLAHAYLF
                     CGPRGVGKTTCARIFAKTINCLNPTPEHEACNQCESCVAFDEQRSYNIHELDAASNNS
                     VDDIRSLIDQVRIPPQIGKYKVYIIDEVHMLSTSAFNSFLKTLEEPPAHAIFILATTE
                     KHKIIPTILSRCQVYDFNRISVADMVNHLQYVAQKENVSAEPEALNIIAQKADGGMRD
                     ALSIFDQTVSYTAGNVTYKSVIENLNVLDFEYYFKLTDAIVAGSVVDCLLVLNDILNR
                     GFEGQYVISGITSHFRDLLVCKDPVTATLFEVGASIREKYVATAKQCSNAFLYKAIEM
                     ANDCDLNYRMSKNKRLLLELTLIKLCQLSENSEDPKNLNPVTPSEKVHPKTKTQPVQN
                     SGTVSEPKPEIKPLPVIPGEKKSTVTTIAGLGVSLSSLTEENPEKPVEKSISLQAEPR
                     QSNRLFTEEELQKAWQEFAENLVEEKLLRNTMMLYKPKMLGNTVFEVEVNTEINKNYL
                     DDYGNVILAYLRESLQNDDITMTIKISEATVVKKPLTSREIFDELVQKNPSLQKLSDE
                     FDLELS"
     gene            11299..11982
                     /locus_tag="PLI30_02115"
     CDS             11299..11982
                     /locus_tag="PLI30_02115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019538307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HOI78379.1"
                     /translation="MKNQRNKIISLSFIGFAVLFCSIQSCKKKEAQQARKVSLYTQAD
                     AVEPRDTVIIDSRLTFLEATAGTKAPKSILDELFLMDVLYRSTDQRLHQGQILTNKKI
                     ENDIRNMFVFMLENDFVIEKAIPIVHYNWDDSLSMDDNNTYSFCYRNVSYSKHARGMA
                     IDINPRFNPLRWKNADYPNQPEGAVLDTTVNGTLYSGHRVVEEFQRLGFRWGHTFSKY
                     YDDHHFEKR"
     gene            complement(11986..>12779)
                     /locus_tag="PLI30_02120"
     CDS             complement(11986..>12779)
                     /locus_tag="PLI30_02120"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HOI78380.1"
                     /translation="KHFSYSNVKVAMKREKVTFKNSRGLILSGAIELPENNSPKAFAI
                     FSHCFTCNKNLINVKYISEALSEKRIAVLRFDFAGLGDSQGNFAETDFSSNIDDLLYA
                     AEFLTLNYEAPKLLVGHSLGGAASIVAAGQLASVKAVSLLGTPSTLDHIKRLFVNKME
                     EITHYGSAMIDVAGRDIEIGRGFVDDLANHNIKKSLNRLNKPLLILHSPEDEMVSIDH
                     ATEIFMAARHPKSFIALDGIDHLIKNIKDARYVGSLIGEWVMRYL"
BASE COUNT         3415 a         2778 c         2834 g         3752 t
ORIGIN      
        1 cagcatattc gagcagcatt ctcgatgtca tttgcgcatt gcaatatatg ccgggagttt
       61 ttctaaaccg ttccgcctga atccggcggg cttttaccac acgtttactg atttctgcac
      121 tgcgttcggc cggagtgctg tcggatagct tttcgaacgc gaccggtacg atttcgatat
      181 ggatatcgat cctgtccagt aaaggtcctg agattctgtt taaatatttc tgtacggctc
      241 ctcccggaca gacacacgct ttatcgggat ggttgtaata accacaaggg cagggattca
      301 tggccgaaac catcataaag ctcgcagggt actccacgga atatttggcg cgggaaattg
      361 ttatttttcg gttttccaat ggctgacgca tcacttcgag aacatttcga ttaaactccg
      421 gaagttcatc cagaaacagc actccgttgt gggccaaact gatttcaccc ggttgaggat
      481 cggctccgcc accgaccatg gctacattac tgatggtatg gtgtggggac cggaatggcc
      541 tgcgcgacat aagggagatg cctttttcca gtttgccggc aacgctgtgt atctttgtgg
      601 tttcgagtgc ctcttcaagg gtgaaagggg ggaggataga gggcattcgt tttgccatca
      661 tcgatttccc tgcgccggga ggccctacca taattatatt gtggctacct gcagccgcta
      721 cttccaacgc gcgtttcaca ttttcctgtc cttttacatc gctgaagtcg aactcgaaat
      781 tcgactggct ctgcaaaaac tcctgcccta tatctgcggt tgttttttca agctcaagtt
      841 cattgtttgc aaaaccgacg acctgacgga tattttctac cccgtaaacc tccaatcctt
      901 ccacaacagc tgcttcttta gcattttccg taggaaggat gaaccctttg aatccgtttt
      961 ccttagccat gatagctata ggtaaggcac ctttgatggg caggatattt ccgtcgagcg
     1021 ataactcacc catgataagg taatcgggca gtttatccgc tattatggtt tcggaggtgg
     1081 ccagtatacc tatcgccagg ggcaggtcgt aagctgatcc ctccttccgg atatccgccg
     1141 gagacatgtt gatcacaatt tgttgccggg gatacttaaa accgcttacc tgaatggcgg
     1201 atacaatcct tttgtggctt tctttcactg atgcatcggg caaacccacc agaaagaact
     1261 tgatcccctt tgtgcaattt acctcaatag ttataagtgt ggcatttata ccttgtactg
     1321 ccgcaccaaa aaccttgacc agcatagctt ttaacgattg ttttttacag aatttttaag
     1381 tgcattttcc actgcgatgg ccgggacgtt tctgtcgctg atgatgccgg caggggaaat
     1441 aaggatatta ttcggaatga actcaatatt gtatgcttcc acgatgtctg atgcccagct
     1501 ttttttctcc ggtacaacgg tccacgggat agaatcgttt tcaatataac tgatgggata
     1561 aatatcggaa tcgatatata cggaaatgaa ctccacgctg tctttgttaa catccctgta
     1621 ggtttgcttg agtgattgaa tcgtttcgcg ggaatcgatt cctgtagttg agatgaaaga
     1681 caacaagagg tattttcccc ggtaatcgta cgattgtatg gtgtcgcccg atttatcaac
     1741 cagccggaag taaggcatag atgagccttc tgcggagcgg ttgatttttt cggaataagc
     1801 cttcaggttc aaccccattt tcgatttcag tatatccccc tgcatatatt ccaatacacg
     1861 gctaagccct ttcggatttt ccgagttggc aaaaaactcg ttgataagaa tcaggctcga
     1921 tatttttgtg ggattttttt tgacctgctc ttcggcattt aatgtcaact cctgattgaa
     1981 agagttgatc ttggctattt cgtcagcatt ggctaagatc ctattattgg acgcggagtt
     2041 cgattctttt tcttctttca ggttacgaag taaaagcgta cgttgttgaa gcagctcttt
     2101 gtttgattct ttaaacaacg tgaggtcgtt gtttatttcg tttccgttta ctttgattaa
     2161 atcggacagt tgcgcatccc ctttgatgga tatgttatct ttcggatggg caaaaaccat
     2221 gactgaagaa ctttggttgt tgaaatacaa cgaaaataca gtaagggtat ctatggtatt
     2281 tttatacgta aatctgccgc gtttatcgct catgatggtg tcgatagcga gggtatcgcc
     2341 ggcgaaataa gaaaccagaa cctggggatt ttccaggctg gagatgtcac ctttaagcgc
     2401 aaccccattt tcgttgtttt tgcaggaaaa caacaatgtg cagataatga ttaatatgga
     2461 caactttaca aacttcactc gattgatttt tttgtttctt ctaactcagc tttacctttc
     2521 cctgattttc caactgttcc tcccgaaata gtaaaacgtt gaaagttgac aaagataaag
     2581 attaatttta agaattctat atattttcac atatttttaa tactttttat aaaatcacag
     2641 aaatggctgg gattacagat aaaatcattt aatttaatta gttttgcact gtaattccaa
     2701 tttcgtgaac atgaattttt ctgttgttat ccctgtttac aatcgtccgg aagaagttga
     2761 cgagctttta gacagtttaa cccgacaatc cggcaagaat tttgaagtaa tcattgccga
     2821 agatgggtcg tctgagaagt gcgatggtat agtccagaaa tatgtatcgc aactccctgt
     2881 tgtttatttt gagaaaccta attccggtcc tgggctgacc cgaaacgctg gtgccaaaaa
     2941 agcgaagtat gactacctca ttttttttga ttccgactgt attatccccg aacactacat
     3001 ggagtatgtc tctttttacc tggaaaggaa ttttgttgat gcctatggtg gccctgataa
     3061 tgcgttgccc tcttttacaa cggtacagaa agctataaat tattccatga cctctttttt
     3121 tactaccgga ggtattcgcg gcggaaaaaa atcgatggat aagtttcatc cccggagttt
     3181 caacctgggg gtctccaaaa aagcgttcga agcccttggc gggtacggta agatgcgttt
     3241 tggggaagac atcgatttca gcctccggct gcttaaaaaa ggatttggaa cggcacttat
     3301 tcccgaagca tatgtttatc ataaacgccg gtcgaccttc aggcagtttt tcaagcaagt
     3361 ttacaattca ggaatagccc ggatcaattt gcacctggcg cacccgggtt cattaagatt
     3421 agtacacttt ctgccatcgt tcttcgtaac agtgatggtt ctttccctgc ttttatcctt
     3481 tttttacccg gtcgttgtgc ttgctcccgc attatacagc ttgctggtac tgatcgactc
     3541 aaccgcaaga aacaactcgt tgagggtcgg cttttacagt attgcagctg cctggacaca
     3601 acttttcgga tacggtgcag gattcatctc tgcgatctgg aaacgattaa tcctgaaaaa
     3661 aggagaattc aacgcttttg agaaaaaatt ttatgagtag tagtctatca acctaaatct
     3721 tctccccgta ggccaaatcg cctgcatcgc caattcccgg tacgatataa gaggattcat
     3781 ttaattccgg atcaatagct gcgacccata tggtggtttt atcttcagga aaatgttggg
     3841 tgcaataatc cacagcctgc tggctggcaa taatggatgc aatatgaatc ctccgaggtg
     3901 ttcccttcgt tagtaaagct ctgtacgtca gctccatgct actgcctgta gccagcatcg
     3961 gatcggtaag gatcaatgtt tttccatcga tactgggcga tgcgatatat tcaatatgaa
     4021 tatcaaacga ttgatgccct gtttccttgt atttccggta agccgacaca aacgcatttc
     4081 cggcacaatc aaaatagttc agcacgccat gatgataagg taatcctgcc cgtaaaatgg
     4141 tgccgataac aatctcttca tcggggacag aaacttcggc cacgtcgaga ggggtggtta
     4201 cggatattgt tttgtagctt aaatccttac tgatttcata agccattatt tcaccgatcc
     4261 tttctaaatt tctacggaat ctgagcctgt cgttctggat ggttatgtct cttatttcac
     4321 ggacaaaaga attcaggagt gagttgtttt tcgaaaaatc aattacattc attgttataa
     4381 taacaaattg ttcatactat cttttttcga aaaacaaagg taatattttt ggtctcaacg
     4441 ataaacattt tcaccgtttt tttgttccat ttacttgagt gtttttttct caggaaagaa
     4501 atttgagtta aaacaggaat aattaaagaa atttttcttt aattacttta ttatctcaac
     4561 ttaattaatt atttttgtcg ccgttttagg atatcgttga tgttttagga tgttattcac
     4621 aagtaaaacg gtttgagcag atagagattt attcacacat ttatattcac aaacaattat
     4681 tattaacatg gcaaatttag atttaagcaa gtatggaatt gtaggcaact tcgagatcct
     4741 tcacaatcct acttacgaag agttatttca ggcggaaatt gatccggcca atgaaggttt
     4801 tgaaaaaggc gtgctgactt caaccggtgc agtagcagtg gataccggaa aatttacagg
     4861 ccgctcaccc aaagacaaat tcatcgtaat ggacgacact accagagaca cgctttggtg
     4921 ggacggtacc atcaaccgcc cgactactcc cgaggtgttc gaccactgta aagatctcgt
     4981 gacaactcag ctttcaacag ctaaaaaatt gtatgttgtt gatacgttct gcggcaccaa
     5041 cgaagatacc cgtatgaaag tgcgttttgt gatggaagta gcctggcagg cacactttgt
     5101 gaccaatatg tttattcgtc cttcacacta cgagctggca aactatggtg agcctgattt
     5161 tgtttgctta aacggttcca aaaccagcaa tccaaactgg aaagaacaag gtctgaattc
     5221 agaagtattt acgttgtttg atcttactcg taaaatgcag gtgattggcg gtacttggta
     5281 cggaggtgaa atgaaaaaag gtatctttgc tctgatgaac tattatttgc cattgaaagg
     5341 tattgcttct atgcactgct cggctaacgt aggtacagaa ggagacgtag ccattttctt
     5401 cgggctttcg ggaacgggaa aaacgactct ttcagcagac ccgaaacgtt atctgatcgg
     5461 tgacgacgag cacggctggg acgatcacgg tgtattcaac tacgaaggtg gttgctacgc
     5521 caaggttatt gatctggaga aagacaaaga acccgatatc tggagagcta ttcgtcgcga
     5581 tgcattgctg gaaaacgtaa ctgttcttcc tgacggaact cccgattact ccgctgccgc
     5641 ttcaaaaaca gagaacacac gtgtttctta ccccatctat cacatcagta aaattgtttc
     5701 tccatctcgc gcaggacatg cgagcaaaat catttacctg tcggctgacg cgtttggcgt
     5761 attacctccg gtatctattt tggatgagaa atcagctcaa tatcacttcc tctccggctt
     5821 tacttcaaaa ctagcaggca ctgaacgcgg tattaccgag ccggttcctt cgttctctcc
     5881 cgcatttggt gaagcattcc tgaccctgca cccgactatg tacgcaaaaa cattgattgg
     5941 aaaagcacaa gaacacaatg caaaagttta cctggtaaac acaggctgga acggaacagg
     6001 aaagcgcatc tctttaaaga atacacgtgc catcattgac gccatcatcg atggttcaat
     6061 tgaagaagct gaaaccattc agattcctat cttgaacctg gttgcgcctg ccgcattgaa
     6121 caatgtggac actgaaattc ttgacccgcg caatacttac gctgatgttg ccgagtggga
     6181 agcaaaagcc aaatcgcttg cggccaaata tatcaaaaac tttgagcaat attgcgacaa
     6241 cgacgatgcg ttagcgttag ttgcgtccgg accgcaattg gactaaacgt aaccgattaa
     6301 aaaaaatccc gcctcctttc gtgagagcgg gattttttat tcagtcaaga aagtcaatcg
     6361 tccagtttca ttatctccac tttcctaaaa tcaatggggt gcccttcgct ttgaagcgag
     6421 atggtccctt tgctcagcat tttgtcgcca ccgttcatca cgataagttt ggcgtaggtc
     6481 gcatcccttt catccaactg tgggcggttg tactgtaaaa ccgtatcgcc gttgataata
     6541 tgactaataa cattgtttcc cctgacctct acttcggccg ttatccaatc ttcaccgtag
     6601 aagttttccg aggaagagtt gatgcaatga tcaaggatca actgatcgtc catgacgata
     6661 tttgttccgg gtgtgcaaac attcatattg gtcctttgca ccagggagtc gctggccagc
     6721 aactgtacct caatggaagt gggaaaatcc tggtcttttt ccattgactc cggtgtctgc
     6781 ccgtgtacca tgattccgga gtttttatac gcccatccgg gtgctcccgg acattgctcg
     6841 ccaacgatac ggtattctac tcgcagttta taatgcgaaa actcatcgtt gtaaaacagg
     6901 tggccgaaac ggtcgcgtaa cgagtcatac tgttcgtaac gtacctttat catgccttct
     6961 tccacccgga atgtattgcc gaaattgtcg cccgcatcgt aacctttaat ttttaccgtc
     7021 caaccggtta aatcggttcc gttgaagaga ggagtccaac cgctggatgc atcgctgctt
     7081 tttttctggc tgcaggatat cagaatgaat gccgcggtcg tggcacccaa aagtgaccat
     7141 acttgtttca tgctatattc tattttttgt tgttgttctt aaaatattcg tcggcaaact
     7201 tgacgaactg tttccagtcg aaaagattca tgttgtggat gccgtctcgg ttatgaaagc
     7261 ccaacggcaa ctgaatgacc tgttcgttgt tcgggggcat gttggcgggt aaacttgttt
     7321 tgaggccaaa caggctgaaa accggttgtg cggcaaccag agccaggtat tgtcctttgg
     7381 ggtccgccca cgaatcatcg gctgcactgg cgacatagac tgcccgggga gccattaatg
     7441 ccagcagcat atgttggtca acaggcaggt ttccttcgtt gttggcgaaa gcagcatatc
     7501 ccggaacaaa ccaatgcggg aagttacggg taattacttc cactgtttct ccaaaattcc
     7561 tgcgggagat ttttgcgccc gagtttcccg actcgttcga tacggcaatg gctacccgtc
     7621 tatcttgtgc tccgcaccaa agtgcagctt tgcccccacg ggaatgtcca acaacaatta
     7681 ccttactggc atctatatcc ttatcggttt caaaataatc gattatccgg ctgacgcccc
     7741 agccccaggc gcctaatgca cgcattccgt ttggttgcag ggtttgctcg ggataaagtt
     7801 tggtgagaat ttgatttacg tagtctgttt tgtgatccgg tgcgacgtcc tttacatcaa
     7861 atccggcaat ggcatagcca gagttcacaa tctcttcggc aggccagaac tcattttcgg
     7921 gtgatgcttg catcgtatcc agtccgcggt ggttaatcgt caggaataca ggaaccggct
     7981 tttttatttt gttggggata aacaagtgga cctgaacagt aataactttt ttgttccgga
     8041 taacgtcaac agctacctgc ttataggtgg cctttccgtt caatgccttg tagttttgtt
     8101 ccaacacctt gaattcgatt ttatcgaaat ccttgggaac ctgcccgtac acgttatcct
     8161 caaaaagttt tagaatttct ggacggcgga ttctgtccca gtcttccgca gacacgattt
     8221 ctttgccgtt ttgagccttt agcagttccg gtaatacata gctgccggct ttggtttcat
     8281 cgtagttctg ggcaaacagc gttgttgtta acgtaaacaa cagcaggtaa agtatctctt
     8341 ttttcatttc catggagttt gaagtgtaaa attattggta aaaggagttc ctgactaatt
     8401 aggctttgca aaaaacgact caatggcttc aatggaggtg tctttaagga tcaccttctt
     8461 gtctttgtcc agcaaataca gcgttggaat ggctttaatg tcgtacagtt tctgtttggt
     8521 aatagttgtt tctttatcgt acgagtgtat ccagtttgca ggcatctgcg gtaaatatga
     8581 tctccattcg tcgatatctg catcgggata aacggttaat acggttatca ttgtgcgggt
     8641 aggcgagttc attgaaaaag ccttgttaag agtgactgac tttgttagca catcggtaac
     8701 agaggcacaa gtagaacatc cgggatttga aaacatcaaa acaaggtatt cacttttgat
     8761 ggagtgcatc ctttgtgatt ctcccgaagc cagtgtgtag gcgaagtcat tggccgtctc
     8821 tcccacgcgg tttttcatta ccatctcgaa ttggaaccgg tacctggatt tatccgtttc
     8881 ggatagaatt tcggaattta ccaactcctg caaaacggga atgtaaaact cttcgttgcg
     8941 gaatggggaa ttggcaccgt agaaatattt atcgaacagc gtgccaaaat aagtatacat
     9001 ggtgttgttc gcttcggctt tccttaacgt gtagttgagc gattctttgg ttctgtcgaa
     9061 cggaacataa gaaagaatat tgatgtaatc cacgaaagcc tgctctgtaa tttcaggacg
     9121 aagtgtgagc ttttcgtcgg aaaaatcgaa cctgtcccag tagtgcatca caagatatac
     9181 ggcacgggca tcggaattgg tcatctcttc agggatgtat ggcaaaacaa aggtgtcggg
     9241 aacgatggtt tgttcccgtt tcaaagaatc ttcaccgata gtgtcgatct gagctttctt
     9301 gctcgatgag caggcataga tcataagaaa agcggtaagt acgaaaaatg attttagaga
     9361 atattgattc ataattcatt cggtttcatc aggctatttg agtcgccttc gctcaagcaa
     9421 gtctggccct gtctcgctta gcgaaatagc tctttttgtt tggtgtaaaa gtacaaaaat
     9481 ttcattgaag ttctcaagct attcatcaaa tgaatagaga ttttgagttt ttttcgtaca
     9541 tttgtctttc ataatttcag gaaaaaatgg acaattatgt tgtttcggcg aggaaataca
     9601 ggccggtgac ttttcgctca gtggtggggc aggaggcatt gaccactacg cttaaaaatg
     9661 ccattacagg aaataaactc gctcatgctt atctgttttg cggcccgcgg ggtgtgggaa
     9721 agacaacgtg tgcccgtatt tttgccaaaa ccatcaactg cctgaatccg acacctgaac
     9781 atgaagcctg caatcaatgt gaatcgtgtg tggctttcga tgaacaacgt tcctacaaca
     9841 ttcacgaact ggatgccgcg tccaacaact ccgtggatga tatccgttcg cttattgatc
     9901 aggtacgcat tccgcctcag atagggaaat acaaagtgta tattatagat gaggttcata
     9961 tgctctccac atcggcattc aactcttttc tcaaaacact ggaggagcct cctgcacatg
    10021 ctattttcat tttggcaact acggagaagc ataaaattat tcctaccatt ctttcacgct
    10081 gccaggtgta tgatttcaac cgcatcagcg ttgccgatat ggtgaatcat ttacaatatg
    10141 ttgcgcagaa ggaaaatgtt tctgccgaac ccgaagcatt aaacatcatc gcgcagaaag
    10201 cggacggggg gatgcgtgat gcgctttcca tttttgatca aacagtaagt tatacagcgg
    10261 gaaatgttac gtataaatcg gtgattgaga acctcaacgt gctcgacttc gaatactatt
    10321 tcaagcttac cgatgccatc gttgcgggaa gtgtggtaga ttgcctgttg gttctgaacg
    10381 atattttgaa ccggggattc gaggggcagt atgtaattag cggaataacg tcgcattttc
    10441 gcgatttact ggtttgtaaa gatcccgtaa ctgcaacatt gttcgaagtg ggagcatcta
    10501 ttcgtgaaaa gtatgttgcc accgcaaaac agtgcagtaa cgcgttttta tataaagcca
    10561 ttgagatggc caacgattgc gacctgaact atcgcatgag taaaaacaaa cgcttgttgc
    10621 tcgagctgac ccttatcaag ctttgtcagt tatcggaaaa ttccgaagat cctaaaaacc
    10681 ttaaccccgt gactccatcg gaaaaagttc atccgaaaac aaaaacacaa cctgttcaaa
    10741 attccgggac tgtttccgaa ccaaaaccgg aaattaaacc cctgcccgta atcccaggag
    10801 agaagaagtc aaccgtaaca acgattgcgg gattgggtgt ttcgttgtca tcattgacgg
    10861 aagagaatcc ggaaaaaccg gttgagaaaa gtatttctct gcaggcggaa cccaggcaga
    10921 gcaaccggct ctttaccgaa gaagagttgc aaaaagcctg gcaagaattt gctgagaatt
    10981 tggtcgagga aaagctgctg agaaatacga tgatgcttta taagcccaag atgctgggaa
    11041 acactgtttt tgaggtggaa gtgaacacgg aaatcaacaa gaattatctg gatgattatg
    11101 gcaacgtaat cttagcctac ttgcgggaga gtttgcagaa cgatgatatt acgatgacca
    11161 ttaaaatttc agaagcaacc gtggttaaga aacccttgac ttcgagagag atttttgatg
    11221 aactggtaca gaaaaaccct tcgttgcaaa agttatccga cgaatttgat ttggagttga
    11281 gctaaaacgg tcgctgtttt gaaaaatcaa cgcaacaaaa taatttccct gtcttttatc
    11341 ggttttgcgg tgttgttctg ttccatccaa tcctgtaaaa aaaaagaggc acagcaggcc
    11401 cggaaagttt ccttgtatac ccaggccgat gccgttgaac ccagagatac cgttattatc
    11461 gactcccggt taaccttcct ggaagcaact gccgggacga aagcgccaaa atctatattg
    11521 gatgaattgt ttcttatgga tgttttgtat agatcaacag atcagaggtt acatcaagga
    11581 caaatactga caaacaaaaa gatcgaaaac gacatacgaa atatgttcgt gtttatgctc
    11641 gaaaatgatt tcgtcatcga aaaagccatt cctattgtgc attataactg ggacgacagc
    11701 ttgtcgatgg acgataacaa cacctacagt ttttgttacc gcaacgtttc ttactcaaaa
    11761 cacgcgcggg gaatggcgat tgatataaat ccgcgtttta atccgttacg gtggaaaaac
    11821 gcagattatc ccaatcagcc cgaaggggca gtgctggata caaccgttaa cgggactttg
    11881 tattctggac atagagtggt ggaggagttc caaagactcg ggttccggtg ggggcacact
    11941 ttttcaaaat attacgacga ccatcatttt gagaaaaggt gaaacttaca ggtacctcat
    12001 tacccattca cctattaaac ttccgacgta ccgggcatct tttatgtttt tgatgaggtg
    12061 atcgataccg tccagggcga tgaagctttt cggatgcctt gccgccataa atatctccgt
    12121 ggcgtgatct atacttacca tttcatcctc gggggagtgt aagataagta agggcttgtt
    12181 cagtctatta agcgacttct tgatgttgtg attcgccagg tcatccacaa agcctctgcc
    12241 tatctcaata tccctcccgg ccacgtcgat catggcactt ccatagtggg tgatctcctc
    12301 cattttattg acgaataatc gttttatatg atccaatgtc gacggagttc ccagcagaga
    12361 aacagcttta acagaggcta gttgtccggc agccacaata gatgcggcac ctcccaagga
    12421 gtgtccgacc aggagtttgg gcgcttcgta attgagcgtt aaaaactcgg cagcgtacaa
    12481 taaatcatcg atgttcgatg agaaatcagt ttccgcaaaa ttaccctggc tatctcccaa
    12541 tcctgcaaaa tcaaagcgaa gtacagcaat tcttttttca ctaagcgcct ccgaaatgta
    12601 tttgacattt atcaggttct tgttgcaggt aaaacagtgc gaaaaaatgg caaaggcctt
    12661 tggtgaattg ttttcaggca attctattgc gccggaaaga atcagccccc ttgaattttt
    12721 gaacgttact ttttctcttt tcatcgctac tttaacgtta gagtaagaga aatgtttct
//
LOCUS       DAOSDR010000029        12644 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrimonas sp. isolate AMR_MDS_1826
            NODE_1917_length_12644_cov_4.379697, whole genome shotgun sequence.
ACCESSION   DAOSDR010000029 DAOSDR010000000
VERSION     DAOSDR010000029.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863355
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrimonas sp. (wastewater metagenome)
  ORGANISM  Petrimonas sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Dysgonomonadaceae; Petrimonas.
REFERENCE   1  (bases 1 to 12644)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12644)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:10:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,720
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 35
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12644
                     /organism="Petrimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1917_length_12644_cov_4.379697"
                     /isolate="AMR_MDS_1826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2023866"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(37..816)
                     /locus_tag="PLI30_02125"
     CDS             complement(37..816)
                     /locus_tag="PLI30_02125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019538147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOI78381.1"
                     /translation="MSTILFHEIVYGPVHSRRMGTSLGVNLLPYDGKLCSFDCIYCEC
                     GFNKDFRTKTKLPDRENVYAAMEDKLQSLRGQNVKLDVITFAGNGEPTMHPQFSGIIE
                     DTVSLRDRYYPEAKISVLSNGINVGKVSVFDTLRKVDNPILKLDSAFDETAKLIDRPN
                     SPNYSVARQVELYKKFRGNFILQTMFLRGEFEGKRLDNSTEKEVSAWLELVRALSPRE
                     VMIYTIDRETPAKQLEKVSLEELRKIAGRVGELGIRTNVAG"
     gene            complement(848..1300)
                     /locus_tag="PLI30_02130"
     CDS             complement(848..1300)
                     /locus_tag="PLI30_02130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019538146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqaA family protein"
                     /protein_id="HOI78382.1"
                     /translation="MLDGLAEYGYWGLLLASFLAATVLPFSSEVVFAALIAAGMDVWT
                     SVFYATIGNAAGGATCYYVGKLGKTEWLERWFKIKPEKIDKTIRWLHGKGAIMGFFGF
                     LPAVGDAILVALGFMRANVPVVMVSMTIGKLLRYVLIGFGTDQIVSWL"
     gene            complement(1303..4095)
                     /gene="polA"
                     /locus_tag="PLI30_02135"
     CDS             complement(1303..4095)
                     /gene="polA"
                     /locus_tag="PLI30_02135"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019538145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HOI78383.1"
                     /translation="MSKQKLFLLDAYALIYRAYYAFIKNPRVNSKGQNTSAIFGFVNT
                     LEEVLKKENPSYIAVAFDPPGPTFRHTEFEAYKAQREATPEDIKASVPLIKKILEAYN
                     IPVLEKEGFEADDVIGTLAKKADKDKFDVYMMTPDKDYGQLTEQHVFIYKPKYGSNEF
                     EILDDKKVMEKYRVSHPSQVIDLLGLMGDTSDNIPGCPGVGPKTAEKLLEEFGSVENL
                     LENSDKLKGTLRTKIEENKEQILFSKFLATIRTDVPIETSIESLKRKELNETAIKEIF
                     EELEFRTLINRVLKIEPQKPIGKQPVQGDLFSGFSDVLEEVKTAPVELPGSFSDIHST
                     RHTYKLLETEQELTELNQQLCAQQSVCFDTETTGIDPLLSDLVGLSFAYTEGEAFYVP
                     ISENREEAQKQVDIFRPFFENDRIEKIGQNLKYDILSLRHYGISVKGKLFDTMIAHYL
                     LNPELRHGMDYMAETYLKYKTIHIEELIGPKGKNQKSMRDVDKQVVCDYAAEDADITL
                     KLKNMLEEEIRQNNFDYLFYEVESPLVYVLADMEWTGVRLDLDALAQLSEEFTAELQQ
                     VEAEIIAMAGEEFNVNSPKQIGEVLFEKMKIIEKPKKTRTGQYSTSEDELQKLRNKHP
                     VIEKILEQRGLKKLLGTYIDAFPLLVNPRTGKVHTSFNQTVAATGRLSSTNPNLQNIP
                     IRDERGKEMRRVFIPDEGCTFLSADYSQIELRIMAHLSEDKNMLEAFNQGLDIHTATA
                     AKINHIPVEEATSDMRRKAKTANFGIIYGISVFGLSERLNIPRGEAKALIDGYFATYP
                     GVKRYMDESIADAREKGYVETVLGRKRFLADINSKNAVVRGYAERNAINAPIQGSAAD
                     IIKVAMIRIFNRLEKEGLQSKMILQVHDELNFNVPANELERMKKIVVEEMENAYKLRV
                     PLKTDCGVGDNWLEAH"
     gene            4190..5164
                     /locus_tag="PLI30_02140"
     CDS             4190..5164
                     /locus_tag="PLI30_02140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019538144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HOI78384.1"
                     /translation="MDESQVIKESLRDELNQFDIYLWKSLENNNSRISEILRHAFKVD
                     GKRIRPMLVFLVAKCCGEITPATYHGAVTVELLHMATLMHDDVVDEASTRRGQPSSNA
                     VFDNKRSVLAGDYVLSSALRESVKTNNLEIIGIISELGQNLAEGELNQYSLVNEIIID
                     EEEYFKVIDKKTASLLYACAKIGAITAGADRVTIDEFGKAGRILGIAFQIRDDIFDYY
                     KADVGKPTGNDIREGKITLPLIYALNHAPQALSDEMMKIIRSCDFSLVNIELLLEFAK
                     SNGGIEYAQKTIDNLLEEAEQIIENVSIDNEFKITLNLLVMYLKNRMV"
     gene            complement(5161..6405)
                     /locus_tag="PLI30_02145"
     CDS             complement(5161..6405)
                     /locus_tag="PLI30_02145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI78385.1"
                     /translation="MDTYTEDYMANVPVYMSYADLRSSVKALPARELEKPGKIYFKDN
                     YLLIVEKFKGIHVIDVSNSANPKNTSFVEVPGCVDIAVKGHILYADSYVDLVAIDVSD
                     ISKIKEVNRLKEVYPYAVPPTDNQLRIGNVEKDKGVVIAWEQKKEHNKLEEHITYPIY
                     YGWDSYGKNSNLSMDAAFGGTVGGISSASFGKGGSMARFGLYDNFLYTVDQYQLFVFN
                     VSEASNPVKVVTQPIGWNVETMFLYDHHMFLGTTNGMLILELVNPSAPRQISSLWHVT
                     TCDPVVVQDGYAYITLRSGVTCNRNANLSVNVLDVVKLSDDYKMNNLIATYNMKAPYG
                     LGIDNNTLFLCDDGLKVFNVADKLNINNNLIAHFKEIETYDVIPLNKFLFTIGDDGFY
                     LYDYSNLQDIKLLGSIPVKEQR"
     gene            complement(6469..7284)
                     /locus_tag="PLI30_02150"
     CDS             complement(6469..7284)
                     /locus_tag="PLI30_02150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI78386.1"
                     /translation="MNKRITLFCLFICLLQYTGAQPVNEALYPRVERGYIELKDGRIL
                     KGKYIYSPDFGKIRIVTFHESVVLDASEVVRITKKKPGTGETNDSGEEFSQTKSYFIF
                     SELGILPGNPDNMNKMPFIFHTTVNYRLLEKLSAGLGAGVEFYKETYLPVTINGIYRF
                     NNNRAAPFAMLQAGYQVPIEGSRTKVVNVMPAHTDYIWPGPQVSYDMELKARGGILLN
                     PSLGIMWQTRSDVNFTFSVGYRFHRLAYRNKEKEYNLNVDYNRLSLKLGLIFN"
     gene            complement(7474..7902)
                     /locus_tag="PLI30_02155"
     CDS             complement(7474..7902)
                     /locus_tag="PLI30_02155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008763145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HOI78387.1"
                     /translation="MTIIRRTNTWLPSVFNDFFGNEWVTNNNKSVPAINIQQNENGFT
                     VEVAAPGMTKEDCNVSVDEDNNLVICFEKKNESEEKDKKGTYLRREFSYTQFTRKMIL
                     PDNVVNEKIAAKVENGVLTVDIPTIKEEEKVSKVKQIDIQ"
     gene            complement(8111..9682)
                     /locus_tag="PLI30_02160"
     CDS             complement(8111..9682)
                     /locus_tag="PLI30_02160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019538142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcineurin-like phosphoesterase C-terminal
                     domain-containing protein"
                     /protein_id="HOI78388.1"
                     /translation="MRKNILLLFALLASFTVYAQQMASGYVFEDNNKNGRKDRREAGI
                     PGVAVSNGIEVTVTNDKGHYSLPAGTDNTIFVIKPSGYGIPVDEHFIPRYYYHHKPEG
                     SPGSFRYKGVPPTGELPGSINFALYKYDEPNDFTTVVFGDPQPYSIQDLDYFSQKIVA
                     DVQKTGKTLFGISLGDIVGDNLDLQPVYKERMKRLQLPWYNVMGNHDMNYDATSDILS
                     DETFQQNFGLANLAFNYGDAHFVILDNILYPDPRGGKGYWAGYREDQLRFLENNLKRV
                     PKNKLIVLSQHIQMKDNTGRSYRLEDRQRIFDLLKDFENVLIMSAHTHLQDQIEYTEI
                     DGWQGKKPLHEYNVGTTSGDWYSGKLDERGLPDASMRDGTPQGYAFLHIKGNQYTVDY
                     KVAGRNPDYQMNIYAPKVVPHNGRTTSQVVVNFFMGSENDAVEYKIDDGQWRPMRYLE
                     AVDPNYTIKLIEWDFTEKLLPGRRPSNAVNSTHLWAGGIQLDLEPGEHTIYVRATDRF
                     GKAHYGQKTYKILAP"
     gene            complement(9918..>12644)
                     /locus_tag="PLI30_02165"
     CDS             complement(9918..>12644)
                     /locus_tag="PLI30_02165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009291646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HOI78389.1"
                     /translation="RWSTGSYLECQDFWRISGIERDVFLWSQPKTSLRDFRVKSTLDD
                     SYKNGIFELETTVSNYSAGVSYAEVSYELLDAAGKTAASGSQAVSVQGQGENAVKFEA
                     QLPNVATWTSEHPNLYKLLISVRKEGEKSGEVVPYSVGFRRFEIKAVKTGERTDRLFL
                     VNGQPIKLKGVNIHETNPKTGHYVPEELMRKDFELMKQNNINTVRLSHYPQARRFYEL
                     CSEFGLYVYDEANIESHGMYYGQESLAKHPEWEQAHMDRTVNMFERNKNHPSVAIWSL
                     GNEAGNGINFFHTYRYLKDQERNFMNRPVNYERGLWEYNTDMYVPQYPSAAWLEEVGK
                     KGSDRPVVPSEYSHAMGNSSGNLDLQWQAIYKYPNLQGAYIWDWVDQGMEAVDENGRV
                     YYKYGGDYGTDMPSDGNFLCNGIVNPDRTPHPAMGEVKYTHQNFAVEAVDLPKGIVNI
                     INRQYFSNTDNYTFKYNITENGKQISEGILPVTLAPQQAAHATVPVGQIKARPGMEYF
                     LNFEVVQKTATQLIPANHIVAVEQFKLPITVPKQAFTDKSQKPELKITSSGKSIVVKS
                     PTVNFVFDKKSGTVTSYKVTGQEYFDKGFGIQPNFWRAPNDNDFGNGNPHRLQVWKQS
                     SRHFNVTDVKGYAQGNNAVVETTYLLAAGNLYTIKYTVHPSGVVKVDVEFHSTDMQAA
                     QLEASEATLMATASPEATAARKASSELVVPRIGVRFRLPASMNAVEYFGRGPGENYID
                     RASGSKVGLYKTTADEMYFPYVRPQENGHRTDTRWVALKGAGNGLMVVADETIGFNSL
                     KNSVEDFDSEEATRRPYQWNNFSSEEIAGRDDADAKNKRPRHTHINDITPRNFVEVCV
                     DMKQQGVAGYNSWGARPLPEYSIPANQNYKWGFTLVPFSNAGDIQIKSVLKY"
BASE COUNT         3092 a         2880 c         2852 g         3820 t
ORIGIN      
        1 ttgacctcag cgatgctggc atctgtcggg tttgttttat cctgccacgt ttgttcggat
       61 tccaagctcc cctaccctgc cggctatttt ccgcaactct tccagggaga ctttttcgag
      121 ctgtttggcg ggtgtttcgc ggtcgatggt gtaaatcatc acctcgcggg gagaaagtgc
      181 tctgacgagc tctaaccagg cagaaacttc cttttcggtg gaattatcca gccgcttccc
      241 ttcaaactcg cctctgagaa acatcgtttg caagataaaa ttaccgcgaa acttcttgta
      301 aagttccact tggcgggcaa ccgaataatt gggagaattc ggacggtcaa tgagtttagc
      361 ggtttcgtca aaagcagagt cgagtttgag aatggggttg tccactttcc ggagagtatc
      421 gaaaacggat accttgccca cgttaattcc attcgacaga acgctgatct ttgcttcggg
      481 ataataccta tcgcgcagcg aaacggtatc ttcaatgatg cctgaaaatt gaggatgcat
      541 cgtcggctcg ccgtttccgg caaaagtgat gacgtcgagt tttacgtttt gcccgcgcag
      601 cgattgcaat ttatcttcca tagcggcgta aacgttttct ctatcgggaa gctttgtttt
      661 tgttctgaaa tccttgttga atccgcattc gcaataaata cagtcgaacg aacaaagctt
      721 gccgtcgtaa ggtaacaggt ttacgcccag tgaagtgccc atccggcggc tgtgaaccgg
      781 gccgtaaact atttcgtgaa aaaggatggt agacatgttg tttagctaaa aaaataatgt
      841 tgaaagatca tagccacgat acaatttgat ccgtcccaaa accgatcaac acgtaccgca
      901 acaattttcc gatagtcatc gaaaccatta ccacgggaac gtttgcccgc ataaacccta
      961 acgctaccag aatggcgtcg cctacggcgg gaagaaatcc gaaaaaaccc attattgcac
     1021 ccttgccgtg caaccatctg atcgttttat cgatcttttc cggttttatt ttgaaccacc
     1081 tctccagcca ttcggttttt cccagtttcc ccacataata acaagtggct cctccggcag
     1141 cattcccgat ggtggcataa aagacagaag tccaaacatc cattcctgcg gcgataagcg
     1201 ccgcaaaaac tacttcagag ctgaacggca gtaccgttgc tgccaggaac gatgcaagca
     1261 atagtcccca atacccgtat tctgctaatc cgtcaagcat aatcaatgcg cttccagcca
     1321 gttatccccc actccacaat ccgttttcag agggacacgg agtttatagg cgttttccat
     1381 ttcctcgaca acaatttttt tcatcctttc cagttcgttt gccggaacgt tgaagttaag
     1441 ttcgtcgtgt acctgaagaa tcattttcga ttgcagtcct tccttttcca gccggttaaa
     1501 gatgcggatc attgccactt ttataatgtc tgccgcactc ccctgaatgg gtgcgtttat
     1561 cgcattcctt tccgcatacc cacgaacgac ggcgtttttg gaattgatgt cggccaaaaa
     1621 acgtttgcgt cccaacacgg tttccacata acctttttcg cgtgcgtcgg caatgctttc
     1681 gtccatatag cgtttcacgc cgggataggt ggcgaaataa ccgtcgataa gtgctttggc
     1741 ttccccacga gggatattca gccgttcgga aagtccgaag accgaaatgc cgtaaataat
     1801 tccaaaattg gcggtttttg ccttgcgccg catgtcggaa gtggcttctt caacggggat
     1861 atgattgatt ttggcggctg tggcggtatg aatatccagt ccctggttaa aggcttccag
     1921 catattttta tcttcgctca ggtgcgccat gatgcggagt tcaatttgcg aatagtcggc
     1981 ggaaagaaac gtgcaccctt cgtccgggat gaaaacgcgt ctcatttctt taccgcgctc
     2041 atcgcgaatg ggtatgttct gcagattggg gttggtgctg ctcagtcgtc ccgttgcagc
     2101 gacggtttgg ttgaaggagg tgtgtacttt tcccgtccgg ggatttacca gtaagggaaa
     2161 agcatcgata taggttccca gcagtttctt caatccccgt tgctccagta ttttttcgat
     2221 aacgggatgt ttgttgcgca acttctgtaa ttcatcttcg ctggtactgt attgcccggt
     2281 ccgggttttt ttcggttttt cgataatttt catcttttcg aaaagcactt cccctatttg
     2341 tttgggtgaa tttacgttga actcctcccc ggccatagcg atgatttccg cttcaacttg
     2401 ttgcagctcg gcggtaaatt cttccgaaag ttgagccaac gcgtctagat cgagacgaac
     2461 acccgtccat tccatatcgg caagtacata aacgaggggc gactccactt cgtaaaagag
     2521 gtaatcgaag ttgttttgtc tgatctcctc ctccagcatg ttcttcaact tcagcgtgat
     2581 atcggcgtct tctgccgcgt aatcgcaaac gacttgttta tccacgtcac gcattgattt
     2641 ttgatttttt ccttttggac cgattagctc ttcaatatgg atggttttgt atttcaggta
     2701 ggtttctgcc atgtaatcca tcccgtgccg gagttcggga ttgaggagat aatgcgcaat
     2761 catcgtatcg aagagttttc cttttacgct aatgccgtaa tggcgaaggg agaggatatc
     2821 gtatttgagg ttttgtccaa ttttttcgat ccggtcattt tcgaaaaaag gcctgaaaat
     2881 atctacttgc ttttgcgctt cttccctgtt ttcggaaatg ggcacgtaaa acgcttcgcc
     2941 ctcagtgtat gcaaacgata ggcccaccag atcgctcaac agcgggtcga ttccggttgt
     3001 ctcggtatcg aagcaaaccg attgctgtgc gcaaagttgt tgattcaact ctgtaagttc
     3061 ctgttccgtt tccagtaatt tatacgtatg cctggtggag tggatgtcgg agaagctgcc
     3121 cgggagttca accggagccg tttttacttc ttccaaaaca tcgctgaagc cactgaacaa
     3181 gtcgccctgg acgggttgtt taccaatcgg tttttggggt tcaattttca gtaccctgtt
     3241 tatcaatgtc ctgaattcca gttcttcgaa tatctctttt atggctgttt cgttcagttc
     3301 ttttcttttc aagctttcga tactggtctc tataggaaca tctgtcctta tcgtggccaa
     3361 aaattttgaa aataaaatct gttccttgtt ctcttcaatt tttgtacgta gcgttccttt
     3421 cagtttatca ctgttttcaa gcagattttc aacggatccg aactcttcca acagcttttc
     3481 cgctgtttta ggtcccacac ccgggcagcc gggaatattg tcggacgtgt cgcccatcag
     3541 gcccagcaaa tcgattacct gtgatgggtg ggaaacgcgg tatttctcca tcactttctt
     3601 atcgtccagt atctcgaact cattactgcc gtatttgggt ttatagataa atacatgctg
     3661 ctccgtcaat tgtccgtaat ccttgtccgg agtcatcata taaacgtcaa acttatcttt
     3721 atccgccttt ttggccaggg ttccgattac atcgtcagct tcgaagcctt ccttttcaag
     3781 caccggtatg ttgtaggctt ccaggatttt tttgatgagg gggactgaag cttttatgtc
     3841 ttcgggagtg gcttcgcgct gtgctttgta ggcctcaaac tccgtatgac ggaatgtggg
     3901 tcccggggga tcgaaagcaa cggcaatgta cgacgggttc tcttttttca gcacttcttc
     3961 caacgtattc acaaacccga aaattgccga tgtattctgt cctttcgaat taacgcgagg
     4021 gtttttaata aatgcataat atgctctgta aatcaacgca taagcatcga gtagaaacag
     4081 tttctgtttg gacatataaa ctaaatattt cttttaaaga cgtaaagtta cgaatatttg
     4141 attgctttaa ggtgaattat ttttaaattt gcgttcaata aaaacaggaa tggacgaaag
     4201 tcaggttata aaggagtcgt tgcgtgacga gttgaatcaa ttcgacattt atctctggaa
     4261 atcgctggaa aacaataatt cccggatatc ggagattctc cggcatgctt ttaaggttga
     4321 cggtaaacgg ataaggccga tgttggtttt tctggtggca aaatgttgcg gggagataac
     4381 gcctgccacg taccacggtg ctgttacggt ggagttgctc catatggcga cgcttatgca
     4441 tgacgatgtg gtggatgagg catcgacacg tcgcggacaa ccatcgtcaa atgcagtttt
     4501 cgataataaa cgttccgttc tggcaggaga ttatgtgttg tcatcggccc tgcgtgagag
     4561 tgttaaaacc aataacctgg aaataatcgg gatcatttcc gaacttgggc aaaacctggc
     4621 ggaaggagag cttaaccaat actcgttggt caatgagata attattgatg aagaagagta
     4681 ttttaaagta attgacaaaa aaacagcttc cctactgtac gcttgtgcaa agatcggtgc
     4741 cattactgca ggtgcagacc gggtaacgat agatgaattc ggaaaagcgg gccgtatatt
     4801 gggtattgct ttccagatcc gtgacgatat tttcgattat tacaaagccg atgtaggtaa
     4861 gcctaccgga aacgatattc gggaaggcaa gatcacgctt ccattaatct atgcactcaa
     4921 ccatgcgccc caagcactat cggatgaaat gatgaagatt atccgttcct gtgatttttc
     4981 gctcgtaaac atcgaactct tgcttgaatt tgccaaaagt aacggaggta ttgaatatgc
     5041 ccagaaaacc atagacaatt tactggaaga ggctgaacag attatcgaaa atgtttcgat
     5101 cgacaacgag tttaaaataa ccttaaacct gttggtgatg tatttgaaga accggatggt
     5161 ttaacgttgt tccttcaccg ggatacttcc caatagtttg atatcttgta aattggagta
     5221 atcataaaga taaaatccgt cgtcgccgat cgtaaacagg aatttgttca gcggaattac
     5281 gtcgtacgtt tctatctctt taaagtgagc tatcaggttg ttgttgatat tcaacttatc
     5341 tgctacatta aataccttta atccatcatc gcacagaaaa agcgtattat tatcgatccc
     5401 caacccgtaa ggcgctttca tattatacgt agctatcagg ttattcattt tgtaatcgtc
     5461 ggatagtttt accacatcga gaacgtttac gcttaggttt gcgtttctgt tgcaggtaac
     5521 accggagcgg agggtaatgt atgcatatcc gtcctgcacc actacgggat cgcaggttgt
     5581 tacatgccag agcgagctta tttgccgtgg agccgatgga ttcaccagct ccagaataag
     5641 catcccgttg gttgttccca aaaacatgtg atggtcatac aggaacatag tttccacgtt
     5701 ccatccgata ggttgggtaa ctacttttac cggattggac gcttccgaaa cattgaagac
     5761 gaatagctgg tactgatcta ccgtataaag aaagttatcg tataagccga accgggccat
     5821 tgacccgcct ttgccaaacg atgcgcttga tattcctccg acagtgccac caaaggccgc
     5881 atccatgctt agatttgagt tttttccata gctatcccat ccgtaataaa tagggtatgt
     5941 tatgtgctct tccagtttat tgtgctcttt cttttgttcc caggcaataa caacgccttt
     6001 atccttttcc acattgccta tccgcagttg attgtcggtg ggcggaaccg cgtaaggata
     6061 aacttccttc aaccggttta cttccttgat tttgcttata tccgaaacat cgatggctac
     6121 cagatctaca tagctgtcgg catataaaat atggcctttt acggcaatgt ctacacagcc
     6181 gggaacctca acaaaagatg tattcttcgg atttgccgaa ttggaaacat cgatcacgtg
     6241 tattccttta aatttctcga caattaacaa gtaattgtct ttgaaataaa tttttccggg
     6301 tttttccagt tccctggcgg gcaaagcctt gacggagctt cttaaatcgg cataactcat
     6361 ataaaccggc acgttggcca tgtaatcttc ggtgtatgta tccatgcagg agctgagagc
     6421 caacaccgaa ataaaacaaa aaaatatttt tatatatttc ataacaagtt agttaaagat
     6481 aagaccaagt tttaaagaga gccggttgta atctacattt aagttatact ccttttcttt
     6541 gttgcggtaa gccagcctgt gaaaacgata accgacagaa aaagtgaaat ttacatccga
     6601 ccgggtttgc cacataattc cgagcgacgg attaagcaaa attccgcctc tggcctttaa
     6661 ttccatatcg taacttacct gcgggcccgg ccaaatgtag tctgtatgtg ccggcattac
     6721 gtttacaact tttgtccggg aaccttctat tggcacctga tatccggcct gaagcattgc
     6781 aaacggggca gcacggttgt tgttgaacct gtaaatcccg tttatcgtta ccggcaggta
     6841 tgtctctttg taaaactcca caccggctcc caatccggca gatagttttt caagcagcct
     6901 gtaatttact gtcgtatgga aaataaacgg cattttgttc atgttgtccg gatttccggg
     6961 taaaatccct aattcactaa aaataaagta gcttttagtt tgtgaaaatt cctccccgga
     7021 gtcattcgtt tcccctgttc ccggtttttt cttggtgatc ctcaccactt ccgatgcatc
     7081 caggacaaca gattcgtgaa aggtgacgat ccttatcttt ccaaaatcag gagaatagat
     7141 gtattttcct ttgagaatgc ggccatcttt caattcgatg tatcctctct caactctcgg
     7201 ataaagtgct tcattgacgg gttgtgctcc agtatattgc aacaggcaga taaacagaca
     7261 aaataaggta atgcgtttgt tcatactatt aatttataaa atccagataa tcgagccgct
     7321 tgtcgttatg tatagggtat agatgaaaaa aatggaaaaa cgttgcatca aaatgtaaaa
     7381 acaattcgat tttaacaatt aggccggata tcccaaaaaa aatatcaccc ctgcctggag
     7441 tgatatttct taacctactt aaggtaaatt gccttattga atatcaatct gttttacctt
     7501 tgataccttc tcttcttctt taattgtcgg tatatctacc gttaatacgc cgttttccac
     7561 tttagcagcg attttctcgt tcactacatt atcgggcaaa atcatttttc gggtgaattg
     7621 cgtgtaagaa aactcgcgcc tcaggtaagt cccttttttg tccttttctt ccgattcatt
     7681 tttcttttca aagcagataa ccaggttgtt atcttcatct acacttacgt tgcaatcttc
     7741 tttggtcatg ccgggagcag ccacctcaac ggtaaagccg ttttcatttt gctgaatatt
     7801 gattgccggc accgacttgt tgttgtttgt aacccattcg tttccaaaaa aatcgttgaa
     7861 aacactgggc aaccatgtgt ttgttcgtct aataattgtc ataattctat cctttctttt
     7921 tagttgttta ttcacttttt tatgtgcatc gccaacgata catcaaaaaa actccaaatt
     7981 atgtgccgat ggaatatttg cgtcttttta agatgattta cgacaaaatg gcttgaaaaa
     8041 agatgatatt tgctaaatgt cgaactatat gctgacaaaa tggcattgtg ttgccgaact
     8101 ttcacccata tcagggtgcc aatatcttgt acgttttttg cccataatgc gctttcccga
     8161 accggtcggt tgcacgtaca taaatggtat gttctccggg ttccaggtcc agttgaatcc
     8221 ccccggccca taaatgtgtg ctgtttaccg cattcgaggg acgccggccg gggaggagtt
     8281 tttccgtaaa atcccactca ataagcttta tcgtgtaatt cgggtcaaca gcttcgaggt
     8341 acctcattgg acgccactgg ccgtcatcga ttttatattc aaccgcgtcg ttttcgcttc
     8401 ccataaaaaa gtttactacc acctgggagg tcgttcttcc gttgtgggga actactttgg
     8461 gagcatatat attcatctga taatcaggat tccttccggc tactttgtaa tctacggtgt
     8521 attggttgcc ttttatatga aggaaagcgt atccctgtgg agtaccgtcg cgcatgctgg
     8581 cgtcgggcaa tcccctttcg tccagtttac ccgaatacca gtcgcccgaa gtggtgccca
     8641 cgttatattc atgcaacggt ttttttccct gccatccgtc gatttcggta tattcaatct
     8701 gatcctgaag atgtgtgtga gccgacataa tcagtacgtt ctcaaaatct ttaagcaggt
     8761 caaagatacg ttgcctgtcc tctaaccggt acgacctgcc cgtgttgtct ttcatttgaa
     8821 tgtgctgcga caacactatc agtttgttct tgggtacccg cttcaggtta ttttccaaaa
     8881 aacggagctg atcctcccgg tatccggccc agtacccttt tccgccgcgg ggatcgggat
     8941 aaaggatatt gtcaagaata acaaaatggg catcgccgta attgaaggca aggtttgcca
     9001 gtccgaaatt ttgctggaaa gtttcatccg acaggatatc ggacgtggcg tcgtaattca
     9061 tatcgtgatt gcccatcacg ttgtaccaag gcagctgaag ccgtttcatc ctttccttgt
     9121 aaaccggttg cagatcgagg ttgtcgccga cgatatcgcc cagacttatg ccaaacaacg
     9181 ttttcccggt cttttgcaca tcggccacga tcttctgaga gaaataatcc aggtcctgaa
     9241 tggagtaggg ctggggatcc ccaaaaacga cggttgtaaa atcgttgggt tcatcgtatt
     9301 tgtacagtgc aaaatttatt gagccgggaa gttctccagt tggcggaaca cctttgtacc
     9361 ggaatgaacc gggtgagcct tccggcttat ggtggtaata ataccggggt ataaaatgct
     9421 catccacagg aattccgtat ccgctaggct ttatcacgaa aatcgtattg tcggttcccg
     9481 ccggaaggga gtaatgccct ttgtcgtttg taacggttac ttcgattccg ttggagacgg
     9541 caacacccgg gattccagct tcgcgacggt ctttcctgcc gtttttattg ttgtcttcaa
     9601 acacatagcc tgaagccatt tgctgggcat aaacggtaaa acttgccagc aaggcaaaaa
     9661 gaagtaaaat gttctttctc attgttgata tttttttgca ttgaattttc atatgtgtga
     9721 ttatatcagc taaatgtcac gtgttaagct gtatacgtca atgataaatg catgttaatg
     9781 cagaattact tatacagatt caacaataaa cttgaggtaa tgtttcggaa ttaagtgaag
     9841 aaaatgtgac gttttcctcc aaaaaaaacg ggaactgttg ttattcaaca ttcccgtttt
     9901 cgaatatatc tgacggatca atacttcaat accgatttga tttgaatatc gcccgcgtta
     9961 ctgaacggca ccagcgtgaa tccccacttg tagttttgat tcgccggtat ggaatattcg
    10021 ggcagcgggc gtgcacccca gctgttgtat ccggctacgc cctgttgctt catatccacg
    10081 cagacttcca caaaattacg tggagtgatg tcattgatgt gggtgtgtct ggggcgctta
    10141 tttttagcat cggcatcgtc gcgtccggca atttcttcgc tgctgaaatt gttccattgg
    10201 taggggcgtc gagtagcttc ttccgaatcg aaatcttcca ctgaattttt cagtgagtta
    10261 aagccgatgg tttcgtcggc aacaaccatc agtccgtttc cagcaccctt aagggcaacc
    10321 cagcgtgtat cggtacgatg tccgttctcc tgaggacgga cgtatgggaa atacatttca
    10381 tcagcggtgg ttttatagag gcctactttt gagcctgagg cgcggtcgat gtaattttca
    10441 ccgggcccac ggccgaagta ttctaccgca ttcatggatg cgggcaggcg aaagcgtacg
    10501 ccaatacgtg gaacaaccag ttccgatgag gccttgcgcg ctgcagtggc ctcgggcgat
    10561 gcggttgcca taagtgttgc ttccgatgct tcaagttggg cggcctgcat gtcagtagaa
    10621 tggaactcta cgtctacttt cactactccc gatggatgaa cggtatattt aattgtgtaa
    10681 aggttaccgg cggccagcag ataggttgtt tcaaccacgg cattattgcc ctgagcgtat
    10741 cccttgacgt cggtgacgtt gaagtggcgg cttgattgtt tccatacctg gaggcggtgc
    10801 ggatttccat ttccaaagtc attgtcgttg ggtgcgcgcc agaagttggg ttgaataccg
    10861 aagcccttat cgaaatattc ctgaccggtt accttgtagg atgtcaccgt tccgcttttt
    10921 ttatcaaaaa caaagtttac tgtaggtgat ttgacaacaa tactttttcc cgacgaagta
    10981 atcttcaatt cgggtttttg ggacttgtcg gtaaacgctt gtttgggtac cgttatcggt
    11041 aatttaaatt gctctacagc cacgatgtga ttggctggaa taagttgggt tgcggttttc
    11101 tgaaccactt cgaaattcag gaaatattcc atccccggcc ttgcttttat ttggccaaca
    11161 ggaacggttg catgagcggc ttgttgcggt gccagtgtta ccgggaggat accttcggaa
    11221 atttgcttcc cgttttctgt aatgttgtat ttaaatgtgt aattgtctgt attggagaag
    11281 tattgccggt taatgatgtt gacaatccct ttcggtaggt caacggcttc aacagcaaag
    11341 ttttgatggg tatatttcac ttcgcccata gcggggtggg gggtacggtc aggatttaca
    11401 ataccgttgc agagaaaatt tccgtcactg ggcatatctg ttccgtaatc gccgccatat
    11461 ttatagtaaa cccggccatt ttcgtcaaca gcctccatac cctggtcaac ccaatcccag
    11521 atgtatgcgc cttggaggtt aggatactta tagatggctt gccactgcag atcgagattt
    11581 ccacttgaat ttcccattgc gtgcgagtat tctgaaggca caacagggcg atcgctccct
    11641 tttttcccga cttcttcgag ccacgctgcg cttggatatt gcggaacgta catgtcggta
    11701 ttgtattccc aaagtccacg ctcgtagttt acaggacggt tcataaaatt gcgttcctga
    11761 tctttcaggt atctataggt atggaagaag ttgattccgt taccggcttc atttccaagt
    11821 gaccatatgg ctacagaggg atgatttttg ttacgctcaa acatgttcac cgttctgtcc
    11881 atatgcgcct gttcccactc ggggtgtttg gccagcgatt cctggccgta atacatgccg
    11941 tgagactcga tgttggcttc gtcgtaaacg tataatccga attcactgca cagttcgtag
    12001 aagcggcgtg cttgtgggta gtgcgacaaa cggacggtgt tgatgttgtt ttgtttcatc
    12061 agttcgaaat ccttgcgcat cagctcttcg ggcacgtagt gccctgtttt tgggttggtt
    12121 tcgtggatgt tgaccccttt tagtttgatg ggttgcccgt tcaccaggaa taaccggtcg
    12181 gttctctcac cggtttttac ggctttgatt tcaaaacggc ggaatccgac ggagtagggg
    12241 actacttcac ccgatttctc gccttctttt cttacgctaa taagaagctt gtagaggtta
    12301 ggatgctccg atgtccaagt ggctacgttg ggtagctgag cttcaaattt tacggcgttc
    12361 tccccttgtc cttgcacact gaccgcttgt gatccggagg ctgccgtctt tccagctgca
    12421 tccagcaatt cgtaagagac ctcggcatac gacacgcctg cgctgtagtt gctaacggtg
    12481 gtttccagtt cgaagatacc gtttttgtaa ctgtcatcaa gcgtggattt cacgcggaaa
    12541 tcgcggagtg aagtcttggg ttgagaccac aaaaacacat cgcgttcaat accgctgatg
    12601 cgccaaaagt cctggcattc aaggtaagat cctgttgacc agcg
//
LOCUS       DAOSDR010000030        12542 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrimonas sp. isolate AMR_MDS_1826
            NODE_1939_length_12542_cov_4.314407, whole genome shotgun sequence.
ACCESSION   DAOSDR010000030 DAOSDR010000000
VERSION     DAOSDR010000030.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863355
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrimonas sp. (wastewater metagenome)
  ORGANISM  Petrimonas sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Dysgonomonadaceae; Petrimonas.
REFERENCE   1  (bases 1 to 12542)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12542)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:10:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,720
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 35
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12542
                     /organism="Petrimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1939_length_12542_cov_4.314407"
                     /isolate="AMR_MDS_1826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2023866"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..589
                     /locus_tag="PLI30_02170"
     CDS             <1..589
                     /locus_tag="PLI30_02170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013610510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="arginine deiminase family protein"
                     /protein_id="HOI78390.1"
                     /translation="ARTSPQAIDLLIQELCREKLSKRHVIVQQLPETPESFIHLDMAF
                     TFLDKNTAMVYKPLILNSNPYKTVHIQIENGKVVKIAPMNNIPAALKKLGMEVEPVIC
                     GGRADDWDQEREQWHSGANFLAIAPGKVISYARNINTLEELNKKGFEIVRADDVISGR
                     FDMETSVKCVVTLDGSELPRGGGGPRCMTMPLRRQ"
     gene            complement(591..1283)
                     /locus_tag="PLI30_02175"
     CDS             complement(591..1283)
                     /locus_tag="PLI30_02175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008773844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YjjG family noncanonical pyrimidine
                     nucleotidase"
                     /protein_id="HOI78391.1"
                     /translation="MKYKNLFIDLDDTLWDIHQNGKECLEEIYSDYGYNRFYPTFDDY
                     YNVYMPSNHHLWNLYRLGEIRKEELIVERFLVPVRKFGINDPGYAKSLSDDFLERTTR
                     KTKLIDGTLDLLDYLRPKYRMHILSNGFREVQFKKIENSGLQPYFDKIILSEDAGVNK
                     PHPGIFTYALKNTNSRRDETLMIGDSWDADIAGAQKSRIAQIWFNPANLSSDGFEPTY
                     TVETLEEIKELL"
     gene            complement(1379..2245)
                     /locus_tag="PLI30_02180"
     CDS             complement(1379..2245)
                     /locus_tag="PLI30_02180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005644425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI78392.1"
                     /translation="MKKVFILLLGAGVLFSCTNFGKKVTESEEYQKLQAERDSLQAVL
                     KTSDAETQEMMAVISEVEANFDKIREAEKYISTQSAQSGEMSQDTKKRVSDNFQMIQE
                     ILKRNKAQLAELNRKYASSNKQVASMQSTIDRLNKEMQESSARLVVLQGELAKRDATI
                     AQLSSDISELAQHAEEQSSTIKEQDKSLHTAYYVFGTANELKEQKILSGGFLKSTKVM
                     QDTFNKDYFLQIDVREVTEIPLYAPKAKIWSTHPEGTYEFVKGANDNLTFQITDTQRF
                     WSLTKYLIIEVN"
     gene            complement(2292..2966)
                     /gene="rsmI"
                     /locus_tag="PLI30_02185"
     CDS             complement(2292..2966)
                     /gene="rsmI"
                     /locus_tag="PLI30_02185"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HOI78393.1"
                     /translation="MGKLVVVPTPVGNLEDMTLRAINVLKSCDLILAEDTRTTGFLLK
                     HFGIETRMQSHHKFNEHQTSIYIAERIKGGETVALVSDAGTPAISDPGFLLVRACVEE
                     DVEVECLPGATAFVPALVMSGLPNDRFCFEGFLPQKKGRQTRLKELAEESRTMVFYES
                     PYRIVKMITQLAEFMGTDRKASVSREISKIFEETIRGTLSELIEHFTAHEPKGEFVIV
                     VEGKSK"
     gene            complement(3069..3731)
                     /locus_tag="PLI30_02190"
     CDS             complement(3069..3731)
                     /locus_tag="PLI30_02190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI78394.1"
                     /translation="MEKKNEYIDRLLQNLQSLEAKVSAIKQDESVSFSFFRDAFQKGQ
                     EVMRLLHELEMFQIEDMKHQMEKLMLFLSESKLRKPEEEVKSIDPPAAQEEKLAHIRK
                     NAYAEGLALPTYTNPRNTESKKEEASEEFSGVIRSVNDVIQAPPPKLDVKRGLSLNDR
                     FYFQRELFDNDREAMNAMMIRLNAFDNYGDTERYLREKTSWDFKDERVKSFLELLKKG
                     FE"
     gene            complement(3796..5220)
                     /locus_tag="PLI30_02195"
     CDS             complement(3796..5220)
                     /locus_tag="PLI30_02195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014225171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOI78395.1"
                     /translation="MINRYFIEKINENFAFDFTADQRSAVAKIVDFLYSRSNNQVFLL
                     KGYAGTGKSSLIGSLVKTMSQFNQKAVLLAPTGRAAKVFSTYAGQPAFTVHKKIYRMD
                     KFTEGPGTFSLAENKHKHTLFLVDEASMISNDNREFSIFGSGRLLDDLMEYVYSGEGN
                     RLLLIGDTAQLPPVQQDFSPALDEKTIESFFFEVVTCTLTEIVRQSEESGVLVNATML
                     RNALRKGNTVDFPKFNVDTFNDVKRITGTELIDEISSAYNRDGIEETVVISRSNKRVN
                     AYNMGIRNTVLYREEELSPGDLLMITKNNYYWASDMENVDFLANGEFVEVVRVRREET
                     MYGFRFCNVLLRHKDYGVEFEAKINLDSLHTEVPGLTREQNEQLFAAINGDYADISRK
                     SARYKKVKENPYFNALLVKYGYAVTCHKAQGGEWKNVFLDLGYVNESHMGDNFYRWLY
                     TSITRSSQMLYLVNLTNDFIEGNK"
     gene            5338..6039
                     /locus_tag="PLI30_02200"
     CDS             5338..6039
                     /locus_tag="PLI30_02200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI78396.1"
                     /translation="MKVVVRVLLVAAIILLAFLCWKSIQGPIDFNAEVKRRDNAVIQR
                     LVDIRTSQVAYRTQKGSYTANFNELADFIKTGKVASVARSGDLTEEQLEAGMTEAKAM
                     SIIRTGDEKAIRAAGLWDESKNAPQLMRDSIYAPAAEVLFPNRRINPDSLAFVPFGNG
                     AKFEMAVDSLITASGYPVQVFEAKTPYTVYLGDLDKKLLNQKIQEVLDRPGDRYPGMM
                     VGSLQVANNNAGNWE"
     gene            6067..6870
                     /locus_tag="PLI30_02205"
     CDS             6067..6870
                     /locus_tag="PLI30_02205"
                     /inference="COORDINATES: protein motif:HMM:NF024271.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3822 family protein"
                     /protein_id="HOI78397.1"
                     /translation="MFLPENIDLAQSEKYTLSIRLMPDGFSFCIFSPADKSVFHYQEK
                     TFSKNLSLIGNIEKTFFEVNFFSQPFKKTFVTIVSPRYTIVPDSYFEWRKAKELFEFN
                     IHGESGKVLNNYISESSCRILFDLDEEVYSFLCRNLWNPSFFSHKARLLPFFANYRVV
                     DRRKRCFVDFHDEMVSVTCFSGSTLLSANTYPDKDKYDALFNIVNVWEKQSLDQNSDL
                     LVLSGNLPDNKESIATLKKLIKNVEELNLLPPGNENYIPTDILLDMDAC"
     gene            6872..7402
                     /gene="rsmD"
                     /locus_tag="PLI30_02210"
     CDS             6872..7402
                     /gene="rsmD"
                     /locus_tag="PLI30_02210"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008824037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052913 - 16S rRNA
                     (guanine(966)-N(2))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HOI78398.1"
                     /translation="MRIISGKYKSRRIPVPANLKARPTTDFARESLFNVLNNRVDWEE
                     TTALDLFSGTGSVALEFVSRGCPFVVNVETNQNHYNFICKAQELLGAKELFPVKADVF
                     KYLQSVKQKFDLIFADPPYDLQSIDTIPDLIFKKELLKPEGVFILEHSRKQNFSAHPH
                     FVSERKYGNVHFSFFE"
     gene            7637..9004
                     /gene="mgtE"
                     /locus_tag="PLI30_02215"
     CDS             7637..9004
                     /gene="mgtE"
                     /locus_tag="PLI30_02215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019538641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HOI78399.1"
                     /translation="MTICNEKDLLKLIKRKEWTTLRNAIHEFDHLELAGIIDRSSEFE
                     SVILFRFLTREQAKNVFQELSHDKQEKIIERLAQHALKVADLLNDMEPDDRTAFLEEL
                     PGKVTQRLMQLLTPENREMATRLLGYPEDSIGRLMTPEYVAVKPHFTVEQTLAHIRQY
                     GQDSETLNVIYVVDNNWRLIDDIRIREIILASPEQMIGELIDYKFSALSAFDDQETGV
                     SMFKDHDRVALPVIDSAGTLLGIVTVDDIFDVAEEEQTEDFHKFGAVQNAIISPLQAT
                     VTFLYKKRVFWLTTLVFVNIFSGAVIAGFENVLASAISLVFFLPLLIDSGGNAGSQSA
                     TLMIRALAMGDVQTRDWIKLLGKEFIVSLLLGLTMAAGVSLVAAVRAPEIILILVITM
                     TLVVIAGSIIGMILPFLFTRLKIDPATASAPLITSIADILGVLIYFTVASKVLVGPTP
                     PFSTY"
     gene            complement(8979..11279)
                     /locus_tag="PLI30_02220"
     CDS             complement(8979..11279)
                     /locus_tag="PLI30_02220"
                     /EC_number="1.1.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009316933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004470 - malic enzyme activity [Evidence
                     IEA];
                     GO_function: GO:0004471 - malate dehydrogenase
                     (decarboxylating) (NAD+) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent malic enzyme"
                     /protein_id="HOI78400.1"
                     /translation="MSTKLREDALRYHAEGRPGKIEVIPTKPYSSQHDLSLAYSPGVA
                     EPCLEIEKDEQKAYEYTSKGNLVAVISNGTAVLGLGDIGAVSGKPVMEGKGLLFKIFA
                     GIDVFDIEIDEKDPEKFIEVVKAISPTFGGINLEDIKAPECFEIEERLKNELDIPIMH
                     DDQHGTAIISSAGLLNALELTGKKIEEVRIVVNGAGAAANSCTKLYMALGAKLENIVM
                     LDSRGVISKNRTDLNERKKPFATERNISTLAEAVAGADVFLGLSVADVLTVEMVQSMN
                     ENPIVFALANPNPEIAYELAIAARKDIVFATGRSDHPNQINNVLGFPYIFRGALDVRA
                     TCINEEMKVAAVRAIADLAKKAVPDVVNAAYNLKRLSFGRDYIIPKPLDNRLLTVVAP
                     AVAKAAIASGVARKPIADWEEYSEILRERMGLDNKMLRRFYDMAKQTPKRVVFSESNH
                     LNMLKAAETCVNEGICYPVLLGNEEKIASIAAENQISLEGVEIVNLRHDREESRRLHY
                     AKLLSEKRSREGYTFQEAAEQMFNRDSFGMMMVESGDADALITGVFGKYLDTINLAKD
                     IIGIREGLNHIGAMHIVNSKRGVLFLADTLFNRHPNSDVLIDIARLANSTVKFFAHDP
                     VIAMLSYSNFGDDKVGSPASVHKVVEALHRDYPDMVVDGEMQVNVALNKDFRDEKFPF
                     TKLKGKNVNTLIFPNLSSANTAYKLLLESGVGDIIGPIQMGLNKPVHILDVGMSVRDI
                     VNMTVIAVIDAIVEENISVNKLKRVE"
     gene            complement(11422..>12542)
                     /locus_tag="PLI30_02225"
     CDS             complement(11422..>12542)
                     /locus_tag="PLI30_02225"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HOI78401.1"
                     /translation="SHFLWHTSHTSFVNGYGNILVNDYPVSDIEARPYPVVPVLPENP
                     DTIAWPMGDLIVDTIPSGIDMQQLNLAVEQAFADTVPYKGTFAVMVVCQGQPVAEKYA
                     DEFSTETLFLSWSMAKSFINALAGILVKEGKLDIYASAGMDEWKNDERRNITIHHLMQ
                     MNSGLEWNEDYGNSSDVNNMLHKEGDMARFASSKPLEYSPGSVFEYSSGSTNIVSYLM
                     RKAIGDDAEYFAFPREQLFNKIGMRSAVFEVDASGTFVGSSYLYASLRDYARFGLLYL
                     NNGNWQGEQILPENWVAYSTTAANGSDRSYGAFFWLNQAGIDYPDVPRDMFSCRGHDG
                     QFIYIIPSKELVIVRTGFSKNGEFDHNGFVAGIVDAIK"
BASE COUNT         3447 a         2973 c         2595 g         3527 t
ORIGIN      
        1 agcacgcacc agtccccaag ccatcgatct attgattcag gagctgtgca gggaaaagct
       61 cagcaaaaga catgttattg ttcaacaatt accggaaaca cccgaatcgt ttatccattt
      121 ggacatggct ttcacctttc tagacaaaaa taccgccatg gtttataaac ctctgatatt
      181 aaacagcaat ccctacaaaa ccgtacacat ccagatcgaa aacgggaaag tagttaaaat
      241 cgcacccatg aacaatatcc cggcagcact aaagaaacta ggcatggagg tcgaaccggt
      301 catttgtggt ggaagggccg acgactggga ccaagagcgg gaacaatggc acagcggagc
      361 caattttctg gcaatcgcac ccggcaaagt aatttcgtat gcccgaaaca ttaacacgtt
      421 ggaagaatta aataaaaagg gatttgaaat tgtacgggcc gacgatgtga taagcggaag
      481 attcgatatg gaaacgtccg tgaaatgtgt ggttacactg gatggatccg aacttccgcg
      541 tggaggcgga gggccgagat gcatgaccat gccactaagg aggcagtaaa ctacaacagc
      601 tcttttatct cttccagcgt ttccacggtg taggttggtt caaacccgtc ggacgaaaga
      661 ttcgccggat taaaccagat ttgtgcgata cggctctttt gtgctcctgc aatatctgca
      721 tcccaactgt cgccaatcat cagggtttca tcccggcggg aattggtgtt tttaagtgcg
      781 taagtgaaaa taccgggatg tggtttattc acccccgcat cttccgaaag aataatttta
      841 tcgaaatagg gctgcagacc cgagttctca attttcttaa actgaacttc acgaaacccg
      901 ttgctgagta tgtgcatccg gtatttcggc ctcaggtaat ccagaagatc caaggtgccg
      961 tcgatcagct ttgtttttcg ggtggtacgc tccaaaaagt cgtcgctcaa cgatttggca
     1021 tatcccggat cattgattcc aaacttcctg accggaacca gaaaacgttc cacaatcagt
     1081 tcttcctttc ttatttcacc cagacggtat aagttccaca gatgatgatt ggatggcata
     1141 taaacgttgt agtaatcatc gaacgtagga taaaacctat tgtaaccgta atccgaataa
     1201 atctcttcca ggcattcctt gccgttttgg tgaatatccc acagggtatc gtccagatcg
     1261 ataaaaaggt ttttatattt catttcgagg accaaaaatc agtaatgctt tagacaacac
     1321 atcctgaacg caataaacgc ccaggatgct atttttttca taatttcccc cttcgctctt
     1381 aattcacctc aataatcaaa tacttcgtga ggctccaaaa ccgttgagta tcggtgatct
     1441 ggaaagtgag gttgtcgttg gctcccttta cgaactcgta agttccctcg ggatgcgtcg
     1501 accagatttt agccttggga gcatacaaag gaatttccgt cacttcacgg acatcgattt
     1561 gcaggaaata atccttgtta aaagtatcct gcatcacttt agtggatttc aggaaacctc
     1621 ccgaaagaat tttttgttct ttcaactcgt tggcagtacc gaaaacataa taagccgtat
     1681 gtaacgattt gtcctgttct ttgatggtcg aagactgttc ttccgcatgt tgagccagct
     1741 cgctgatgtc actcgacaat tgggcaatgg tcgcatcgcg tttagccaat tccccttgca
     1801 gtaccaccag ccttgcagaa ctttcctgca tttccttgtt caaccggtca atcgtacttt
     1861 gcatcgacgc cacctgcttg ttgctggaag catacttcct gttcagttcc gccagttgag
     1921 ctttgtttcg tttcaaaatc tcctgaatca tctggaagtt atcgcttact ctttttttag
     1981 tatcctgact catctccccg ctttgcgcgg attgagttga aatgtacttt tccgcttcac
     2041 ggatcttatc gaaattcgct tcaacttcac tgatcacagc catcatttcc tgggtttcgg
     2101 catccgacgt tttcaaaacc gcctgcaatg aatctcgttc tgcctgcaac ttttgatatt
     2161 cttcggattc ggtcactttc tttccaaaat tggtacaaga aaacaaaact cctgcgccga
     2221 gcaatagaat gaatactttt ttcataattc aatactttta ttgttattaa acgaattcag
     2281 ttcaaaaatg tttattttga ttttccctct acaacgatca caaattctcc tttgggttca
     2341 tgagccgtaa aatgctcgat aagttccgac aatgttccac gaatggtttc ttcgaatatt
     2401 ttggatattt cccgcgaaac ggatgctttt ctatctgtgc ccataaattc agccagttgc
     2461 gttatcattt ttactatccg gtaaggtgac tcgtagaaaa ccatcgttcg tgactcttca
     2521 gcgagctctt ttaaccgggt ttgcctacct tttttctgcg gtaaaaaccc ctcgaagcaa
     2581 aaccggtcgt tgggaagccc cgacattacc agagccggaa caaatgctgt tgctcccggc
     2641 aagcactcca cctcaacatc ttcctctaca caggcacgga caagcaggaa tcccggatcg
     2701 gaaatcgccg gagtaccggc atccgatacg agcgcaacgg tctctccccc tttgatccgc
     2761 tctgcaatat aaatggacgt ttgatgttca ttaaacttgt gatgcgattg catccgggtt
     2821 tcaatgccga aatgtttcaa taggaaaccg gttgtccgtg tatcttctgc aagaattaaa
     2881 tcacaacttt tcagcacgtt aatcgccctc aacgtcatat cttccagatt tccgacaggg
     2941 gtcggtacaa ctaccagctt tcccatttta attgttattc ggtttttatt tagctgtaaa
     3001 tcaatcgcta ttcgatcgtt tgggagaaaa catcaacaca gcgactcata acgcagagca
     3061 attacaattt actcaaaacc ctttttcagc agttccaaaa aacttttcac cctctcgtcc
     3121 ttaaaatccc acgaggtttt ttctcttaaa taccgttcgg tatctccata attatcgaat
     3181 gcattgaggc ggatcatcat cgcattcatc gcttcacgat cgttatcaaa taattctcgc
     3241 tgaaaataaa accggtcatt caggctcagc ccgcgtttga catccaactt gggaggaggt
     3301 gcctgaataa catcgtttac cgaacggatc acaccggaaa actcctcaga ggcctcttct
     3361 tttttcgatt ctgtattcct gggattggtg tatgtgggta aagcgagacc ttccgcataa
     3421 gcatttttcc ggatgtgggc taacttctcc tcctgcgcag caggcggatc aatacttttc
     3481 acctcctctt caggctttcg cagtttactt tccgacaaga acaacatcag tttctccatc
     3541 tggtgtttca tatcttctat ctgaaacatt tccagttcat gaagcaaacg catcacctcc
     3601 tgccccttct gaaacgcgtc gcgaaagaaa gagaaagaaa cagactcatc ctgtttgatg
     3661 gcggaaacct ttgcttcaag gctttgtaag ttctgaagca gcctatcgat atattcgttt
     3721 ttcttttcca tgattatccg gtttcagttg cttttttgtt aaaaaacggg ataaattcga
     3781 ccacaaaagt aatcattatt tgtttccttc aataaaatcg tttgtcaaat tcacaagata
     3841 taacatttgt gaacttcttg ttattgaagt gtaaagccag cggtagaaat tatcgcccat
     3901 atgcgattca ttcacgtacc ccaggtccag gaaaacgttt ttccattctc caccctgcgc
     3961 tttgtggcag gtaacggcgt atccatactt cacaagaagt gcattaaaat aggggttctc
     4021 ctttactttt ttgtaccggg cacttttccg ggaaatatca gcataatctc cgtttatggc
     4081 tgcgaagagc tgttcgtttt gttcacgggt taggccgggc acctcggtgt gcaagctatc
     4141 aagattaatt tttgcttcga actccactcc gtaatctttg tgccgaagca ggacattaca
     4201 aaaacggaat ccatacattg tttcctcccg gcgaacacgt acgacctcca caaactcgcc
     4261 attggccagg aagtcaacat tttccatatc acttgcccaa tagtaattgt tttttgtaat
     4321 catcagcaaa tcacccggcg aaagttcctc ttcccggtaa agtaccgtat tacggatacc
     4381 catattgtaa gcgtttaccc gtttattgga acgcgaaatc acaacagtct cctcaatacc
     4441 gtcacggttg taagccgacg agatctcatc aataagctcc gtgccggtta ttcttttcac
     4501 atcgttgaat gtatcgacat tgaatttggg aaagtcaaca gtattaccct tccgcaaggc
     4561 attacgcaac atcgttgcgt tgaccagaac tcccgattct tctgattgac gcactatttc
     4621 ggtgagtgtg caggtaacga cttcgaaaaa aaaagattca atcgtttttt cgtcaagagc
     4681 cgggctgaaa tcctgctgca caggtggtaa ttgagccgta tcaccaataa gtagtagccg
     4741 gtttccttcg ccggaataaa catactccat caggtcatcc agcaaccggc ccgatccaaa
     4801 aatggagaac tctctgttgt cgttgctgat catagatgct tcatccacga gaaacagtgt
     4861 atgtttgtgt ttattctcgg ctagtgaaaa agtaccgggt ccttcagtaa acttgtccat
     4921 ccggtaaatc ttcttatgaa cagtgaatgc cggctgaccg gcatatgtcg aaaaaacctt
     4981 cgccgcacgt cccgtcggag ccagcaaaac ggctttttga ttgaattgcg acatcgtttt
     5041 aaccaaacta ccaataaggg aagacttgcc tgtccccgca tatcctttca gcaaaaagac
     5101 ctgattattt gaccgcgagt aaagaaaatc aactatcttt gcaaccgcag aacgttgatc
     5161 ggcagtaaaa tcgaaagcga aattttcgtt gattttttcg ataaaatacc gatttatcat
     5221 ttttttgagt tatttttgtg gtaaatgtag cacatttatt attctttttc taaatttgta
     5281 ctctaaaatc aaaaataata atttaaaaac tcaataaaaa attaaaaaca aacatccatg
     5341 aaagttgtag taagagtgct tttagtagca gccatcatcc tattagcttt cctttgctgg
     5401 aaaagtattc aaggcccaat tgacttcaat gcagaggtaa aacgtcgcga caacgcagtc
     5461 atccagcgtc tggttgacat tcgcacatcg caggttgctt atcgtacgca aaaaggttca
     5521 tatacggcta atttcaatga attggcagat ttcatcaaaa ccgggaaggt agcatctgtt
     5581 gccagatcgg gagaccttac cgaagagcaa ttagaagccg gaatgactga agcgaaagcc
     5641 atgagcatta tccgcaccgg agacgaaaaa gcgatccgtg ctgcaggctt gtgggatgaa
     5701 agcaaaaatg ctcctcagct tatgcgtgac tctatctatg ctcctgctgc cgaagtactt
     5761 ttcccgaaca gaagaatcaa tcccgattca ttggccttcg ttcctttcgg aaacggtgca
     5821 aaatttgaaa tggcagtgga ttctctgatt acagcctctg gttatccggt acaggttttc
     5881 gaagcaaaaa ctccttacac ggtttatttg ggcgatctgg acaagaaact gttgaaccag
     5941 aaaatccaag aggtgttgga tcgtccagga gacaggtatc cgggtatgat ggttggatcc
     6001 cttcaagtgg ctaacaataa cgccggaaac tgggaataat ttcagcagta aacactgcac
     6061 tgggttatgt ttttaccgga aaatattgat ttagctcaat ctgaaaagta tactttatcc
     6121 attcggctta tgccggatgg attttctttt tgtatctttt caccagccga taagtcggtg
     6181 ttccattacc aggaaaaaac attcagtaaa aacttatctt taataggaaa catcgaaaaa
     6241 acattttttg aggtcaattt tttctcacag ccttttaaaa aaacctttgt aacgattgta
     6301 tcaccccgtt acacgatcgt tcctgactcc tattttgaat ggagaaaagc aaaagagctt
     6361 tttgaattta atattcacgg agagagcggg aaggttttaa ataattacat ttctgaaagt
     6421 agctgccgta tcctttttga tttggacgaa gaggtttatt cgtttctatg taggaacctg
     6481 tggaatcctt cctttttcag ccataaagcc cggcttcttc ccttttttgc caactatcgg
     6541 gtggtagaca ggcgaaaaag atgttttgtg gattttcacg atgaaatggt ttccgtaacc
     6601 tgcttttcag ggtcaacgtt actgtctgcc aacacctatc cggataagga caaatatgat
     6661 gcgctattca atattgtgaa tgtatgggaa aaacaatccc tggatcaaaa ttccgatctt
     6721 ttggtccttt cgggaaattt accggataac aaagaaagca ttgccacatt gaaaaaactc
     6781 attaaaaacg tggaagagct caatttgctc cctcctggaa acgaaaacta tattccaacc
     6841 gatattcttt tggatatgga cgcctgttaa tatgcgtatc atcagtggaa aatataagtc
     6901 gaggcggatt ccggttcctg caaaccttaa agcaaggccc acaactgatt ttgcgcgtga
     6961 gagcctgttt aatgtactca ataaccgggt ggactgggaa gaaactacag ccctggattt
     7021 gttttctgga acaggcagcg tagccctgga atttgtatcc cgcggatgtc cgtttgttgt
     7081 aaacgttgag acgaatcaaa accactacaa ctttatttgc aaagcacagg agttactggg
     7141 tgcgaaagag ttattcccgg taaaagccga tgtgtttaaa tacctccaat cggtaaaaca
     7201 gaagttcgac cttatctttg cggatccacc ttacgattta cagtccatag acactattcc
     7261 cgaccttatt ttcaaaaaag aactcctgaa acccgaggga gtttttattc tggagcattc
     7321 cagaaaacaa aacttttccg ctcatcctca ttttgtatcg gagagaaaat acgggaatgt
     7381 gcatttttca ttttttgagt aataatctta catcgctcca tattgtcgtt aacctaaatg
     7441 ccgaaaggaa tctaaaacat cactttggat ttcaaattga cacagtaaat cctgttgcac
     7501 aaccaaacat cctgaaacat cgcgtcattt tgatttaatt tgattacctt tgtcttttca
     7561 aacaaggcag tgattctcac cgccctttcc tgtaagagca ggacctccag acaggaaaga
     7621 cacaaagaaa actattatga ctatatgtaa cgaaaaagac ctgcttaaac taatcaaaag
     7681 gaaagaatgg acaacgttgc gaaacgccat acacgaattt gatcaccttg aacttgccgg
     7741 cattatcgac cgaagtagcg agtttgaatc agtgatattg ttccgttttc tgacgcgtga
     7801 gcaagcaaaa aatgtttttc aggagctttc gcacgacaag caggaaaaga ttatagagag
     7861 actggcgcaa catgctttaa aagtagccga tctgctcaac gatatggagc cagacgatcg
     7921 cacagctttt ctagaggaac ttcccggaaa ggtaacacaa cgtcttatgc agttgcttac
     7981 tcccgaaaac agggagatgg caaccagact tcttggatat cccgaagaca gcataggtag
     8041 gctgatgaca ccggaatacg tagccgttaa accccacttc acggttgaac aaacgctggc
     8101 gcatattcgc caatacggac aagactccga aacactgaac gtaatttacg tagtggacaa
     8161 caactggcgc cttatcgacg atattcgcat acgtgaaata atcctggcct cgcccgaaca
     8221 gatgatcgga gaacttatcg attataaatt ttcggcactg agcgcattcg acgaccagga
     8281 gaccggtgta agcatgttca aggaccacga cagggtagca cttcccgtga tcgatagtgc
     8341 cggaacgctt ctgggcattg taaccgttga tgatattttt gatgtagccg aagaagaaca
     8401 aaccgaggac tttcacaagt tcggcgccgt acaaaacgcc attatcagtc cgttacaggc
     8461 aaccgttact tttttgtaca agaaacgggt attctggctt acaacactgg ttttcgtaaa
     8521 tatcttttca ggagcagtca tagccggctt tgaaaacgtg ctggcttccg ccatatccct
     8581 tgtttttttt cttcctttgc ttatcgacag tggcgggaat gccggctctc aatccgcaac
     8641 acttatgatc cgtgcattgg ccatgggcga tgttcagaca agggattgga taaaactgtt
     8701 gggaaaagag tttattgttt cgttactctt aggcctgaca atggctgcag gagtaagttt
     8761 ggtagcagca gtccgtgctc ctgaaataat tttgattctc gttataacca tgacgctggt
     8821 ggttattgcg ggaagcatta tcgggatgat cctgcccttc ctattcacga ggctgaaaat
     8881 tgatccggca acggcaagcg ccccccttat aacatccatc gcagacatat taggtgtact
     8941 catctatttc accgtggcca gcaaagtgtt ggtcggccct actccaccct tttcaactta
     9001 ttgacagaga tattctcttc tacaatagca tcaataacag caataaccgt catgttcaca
     9061 atgtcgcgga ccgacatgcc cacgtctaaa atatgaaccg gcttgttcaa tcccatctgg
     9121 attggaccga taatgtcgcc gactccactt tccagaagta gcttatatgc tgtgtttgcc
     9181 gaactcaggt taggaaatat caacgtattc acattttttc ctttcagctt ggtgaaagga
     9241 aacttctcat ccctgaagtc tttattcaac gccacgttca cctgcatttc accatccacc
     9301 accatatccg gataatcccg gtgtaatgct tccaccactt tgtggacact ggcaggactt
     9361 cccactttgt cgtcaccaaa attggaatag gagagcatgg caattaccgg atcatgagcg
     9421 aaaaacttta ccgtagagtt tgccaaacgg gcaatatcga tcagaacatc ggaatttgga
     9481 tgacggttaa aaagcgtatc ggcaagaaag agcacgcctc tcttcgagtt gacaatgtgc
     9541 atggctccaa tgtgattaag gccctcacga atgccaataa tatctttggc gagattgatg
     9601 gtatcgaggt atttcccgaa aacaccggta ataagtgcat ccgcgtcgcc agactccacc
     9661 atcatcatcc cgaaagagtc gcggttaaac atctgctcgg cagcttcctg aaaagtatac
     9721 ccctcgcggc tccgcttttc cgaaagcaat ttcgcgtaat gcaaccgcct ggattcttcc
     9781 ctatcgtggc ggagattaac aatttctact ccctctagac tgatctgatt ttcggcagca
     9841 atgctggcta ttttttcttc attgcccagt aaaaccggat aacagatacc ctcgttaaca
     9901 caggtctcag cagctttgag catattcaag tggttggatt ccgagaaaac cacccgttta
     9961 ggggtttgtt ttgccatatc gtagaaacgg cgcaacattt tattgtccaa ccccatccgt
    10021 tcacgaagaa tttcgctgta ctcttcccag tcggcaatgg gtttccgggc aactccagac
    10081 gctatcgccg ctttggcaac tgctggtgca acaactgtca gcaaccggtt gtcgagtggt
    10141 tttggaatga tataatcgcg gccgaaactc aaccgtttca agttataagc ggcattcacc
    10201 acatcgggaa ctgccttttt tgccagatca gcaatagcac ggacagcggc aaccttcatc
    10261 tcttcgttaa tgcaggtagc acggacatcc aacgccccac ggaaaatata gggaaaaccc
    10321 agcacattgt ttatctggtt gggatgatcg gaacgtcccg tcgcaaaaac aatatctttc
    10381 cgggcggcga tagccaattc atacgctatt tccggattag ggttcgccaa tgcgaagaca
    10441 atcggatttt cattcatcga ttgaaccatt tcaacggtca gcacatcggc caccgaaagt
    10501 ccgagaaaga catccgcccc tgcaacagct tctgctaagg ttgagatatt ccgttcggta
    10561 gcaaacggtt ttttccgttc gttcagatcg gtcctatttt tcgatattac tccccgagaa
    10621 tcgagcatca cgatattttc caatttggct cccaacgcca tgtaaagttt tgtgcaggaa
    10681 ttggctgctg cacccgcgcc attcaccaca atcctcacct cttcgatctt ctttcctgtc
    10741 agttccagcg cattaagcaa tcctgcagat gaaataatgg ctgttccgtg ttgatcgtca
    10801 tgcatgatag ggatatccag ttcatttttt aaccgttctt caatttcaaa acattcgggt
    10861 gctttgatat cttccagatt aatccctcca aaagtgggag aaatagcttt gaccacttcg
    10921 atgaattttt ccggatcttt ctcgtcgatc tcgatgtcaa aaacatctat tccggcaaag
    10981 atcttaaaaa gcagcccttt cccttccatc accggttttc ccgaaacagc accgatatca
    11041 cccaaaccca gcactgctgt gccgttagag ataaccgcca ctaggttccc tttggaagta
    11101 tattcgtaag ctttttgctc atccttttct atttcgaggc acggttcggc cacaccgggc
    11161 gaatatgcca gtgataagtc gtgttgggaa ctgtagggtt tggtgggaat cacctctatt
    11221 tttccgggac gcccctcggc atgatacctg agagcatctt cgcgtaattt agttgacatt
    11281 gtgcaggatt tttaagtata acctaaataa ttacacaaat ttacgcaatg aaatttaata
    11341 tatgaaagat tttgaaagat ttttgtaaca aaacctcagc aaccgacctc tcaagtttgg
    11401 taacagtgag gtttgattgg gttatttaat agcatcaaca attcccgcta caaatccgtt
    11461 atggtcaaac tctccatttt ttgaaaaacc ggtccggacg atcaccagtt ctttcgatgg
    11521 aataatatag ataaactgcc cgtcatggcc gcggcagcta aacatatcgc ggggcacatc
    11581 gggatagtcg atgccagctt ggttcagcca gaaaaatgca ccgtaacttc tatcggaacc
    11641 gtttgccgca gtggtgctgt atgcaaccca gttttctggc aggatctgtt caccctgcca
    11701 gttgccgttg ttcaggtaaa gtaagccaaa gcgcgcatag tcacgtaagg aggcatagag
    11761 gtaggacgac cccacaaaag tacccgatgc atcgacctca aaaacagcac tgcgcatccc
    11821 aattttgttg aaaagttgtt cacgcggaaa agcgaaatac tctgcatcgt ctccaattgc
    11881 cttgcgcatc aggtaagaaa caatgttggt agatcccgat gagtattcaa ataccgaacc
    11941 gggggaatac tccagtggtt tcgaactggc aaaccgcgcc atatcaccct ccttgtgcaa
    12001 catgttgttc acgtcggagc tgtttccata atcttcgttc cattccaggc cgctgttcat
    12061 ctgcatcagg tggtggatgg tgatgttacg cctttcgtca ttcttccact catccatgcc
    12121 ggcagacgca tatatatcca gtttaccctc tttcaccaga ataccggcca gggcattgat
    12181 aaagcttttg gccatcgacc agctcagaaa aagggtttcg gtactaaatt catcagcata
    12241 tttttccgca accggttgtc cttgacaaac aaccatcacc gcaaatgtac ccttgtaggg
    12301 gacagtatcg gcaaatgcct gttccaccgc aaggttgagt tgttgcatgt cgataccgga
    12361 agggatggtg tcgacaatga ggtcccccat gggccatgca atcgtgtcgg ggttttccgg
    12421 taaaaccggc acaaccggat aaggacgtgc ctctatatca ctcacggggt agtcgtttac
    12481 caggatgttg ccatagccat tcacgaaaga agtatgcgaa gtatgccaga gaaagtggct
    12541 tg
//
LOCUS       DAOSDR010000031        12394 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrimonas sp. isolate AMR_MDS_1826
            NODE_1970_length_12394_cov_4.237053, whole genome shotgun sequence.
ACCESSION   DAOSDR010000031 DAOSDR010000000
VERSION     DAOSDR010000031.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863355
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrimonas sp. (wastewater metagenome)
  ORGANISM  Petrimonas sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Dysgonomonadaceae; Petrimonas.
REFERENCE   1  (bases 1 to 12394)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12394)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:10:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,720
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 35
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12394
                     /organism="Petrimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1970_length_12394_cov_4.237053"
                     /isolate="AMR_MDS_1826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2023866"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..453)
                     /locus_tag="PLI30_02230"
     CDS             complement(<1..453)
                     /locus_tag="PLI30_02230"
                     /inference="COORDINATES: protein motif:HMM:NF025679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4382 domain-containing protein"
                     /protein_id="HOI78402.1"
                     /translation="MKAILTLFSLFLFAISFTGCSKNDPSINATRLRIKLTDAPVLLI
                     SEFNVDIQKIEISTTDNTTSDEKWTTLDFQGGVYNVLPLSNGKSKQIIDQFFPAGVLR
                     KIKITFGNNSKIKTDTGEKDLILDSEIKDGIIVEVNENLYASYVTSIMI"
     gene            597..1196
                     /locus_tag="PLI30_02235"
     CDS             597..1196
                     /locus_tag="PLI30_02235"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HOI78403.1"
                     /translation="MEDLTTEQKIAAAARKIFTQKGYAATKTRDIAEEAGINLALVNY
                     YFGSKENLFKQVLKEKFEALFGLMMPVLSDRDISLDEKIRLLVKNYTKLLIENEELPI
                     FVLNELRNNENIFNPILQQARTISQPTIDEQLQHNGFAISTPDFIMNIVSLTIFPFIA
                     KPLFITSGMVKEEDFLQFIENREKLIPEWIFAILKQSQQ"
     gene            1193..2476
                     /locus_tag="PLI30_02240"
     CDS             1193..2476
                     /locus_tag="PLI30_02240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009317274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HOI78404.1"
                     /translation="MKYRRFTLFIPLFFIPVFTVNAQSLTIEDCQGMARENYPAIARF
                     NVIENIRSFNLENANRAYYPQLSLNAKATWQSDVTKIDLELPSNFPPLEIPIPDKDQY
                     QVYGQLNQLIWDGGKVSAQKEMIVANAEVEKQKLETEIYSLQERVNQVFFGILLLNEQ
                     LTQQGILEKELQRNLEKVQSYMLNGVANDADLSAVKVEQLKTNQQRIQMESALDSYIK
                     ILSVLTGHRIDPKTVFVKPPVAEVPDHVVIDRPELRLFTAQENLLNSQKTALTARNRP
                     VISAFAQGGYGKPALNMFENKFKPYVIGGVSFSWNLGSLYTYKQEKKSIEQQKLTLDS
                     ERETFLYNLNVQLPQQQNEIKKYRHTMQDDDEIIRLRKQIKDAAEAKVENGTLTVSDL
                     LQEINALEVARQAKSLHEIQYLMSVYKLKFTTNLN"
     gene            2500..3417
                     /locus_tag="PLI30_02245"
     CDS             2500..3417
                     /locus_tag="PLI30_02245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009040507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HOI78405.1"
                     /translation="MKPTKLFIFPVILFLLASCSGNDSDYDATGSFEATEIIVSAQGS
                     GEILSLDIAEGQTLQAGEVVGQIDTVQLFLQKMSLESNIQGVRVQRPNIGAQTAAIEK
                     QIQTLQREHTRTQNLIEANAANKKQLDDINAQIEVLQKQLSAQTSTLQKSSENIYAQS
                     SALQIQVAQLDDLLKKCTIKSPASGVVLNKYAERGELAGTGSPLFKIADIDNMYLRAY
                     ITSDQLSGIKLNDSVTVRVDAGEGDMKSYPGTVSWISGKSEFTPKTIQTKNERANLVY
                     AIKIAVKNDGFLKIGMYGEVKFRNLEGRP"
     gene            3414..4316
                     /locus_tag="PLI30_02250"
     CDS             3414..4316
                     /locus_tag="PLI30_02250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOI78406.1"
                     /translation="MITATNLHKTYGKVTALNDLSFSVNDGEIFGVIGPDGSGKTTLF
                     RILASLLLPDSGSAGINGWDLVKDFRKIRCIIGYMPGRFSLYPDLSVEENLKFFATVF
                     NTTLEENYPLIKDIYQQIEPFKKRRAGALSGGMKQKLALSCALIHKPGVLILDEPTTG
                     VDPVSRKEFWDMLERLKSEGITVLVSTAYMDEASLCDRIALMREGSFIATDTPQNIIN
                     RYTETLWTVQTENLSASLHDLRAHPKVKTCFAFGNEHHVTVQPDLPVAGLQYYLKEKG
                     YSKVQINVTTPTVEDCFMALTSET"
     gene            4320..5069
                     /locus_tag="PLI30_02255"
     CDS             4320..5069
                     /locus_tag="PLI30_02255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014225560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOI78407.1"
                     /translation="MESDIVINVSGLTKKFGNFTAVDRISFDVRKGEVFGFLGANGAG
                     KTTAMQMLCGISKPTSGKGTVAGYDILKENEKIKRSIGYMSQRFSLYEDLKVWENIRL
                     FGGIYGLTDKEIKEKTDELLHRLRFESERNTLVKSLPLGWKQKLAFSVAVFHTPQIVF
                     LDEPTGGVDPAMRRQFWEMIYEASDRGITVFVTTHYMDEAEYCHRVSIMVDGRIETLD
                     SPGNLKEIHQAESMDEVFRKLARKATRMDNG"
     gene            5082..6167
                     /locus_tag="PLI30_02260"
     CDS             5082..6167
                     /locus_tag="PLI30_02260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005788223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOI78408.1"
                     /translation="MKQFFSFVHKEFYHILRDGRTMLILLGMPVVQILLFGFAINMEV
                     QHIRTVVFDPAQDAATRDITERLMANPYFHMQGYVYSPDEINKLLQRGETDVAIVYEQ
                     NFNENLVHSKKAQLQIIADASNPNTGTIVANYVTAIVSEYRPHIPYRIEVENKLLYNP
                     EMKSAYTFVPGIMGLVLMIICAIMTSISIVREKERGTMEVLLASPLKPGTVLISKTVP
                     YLALSFINLITILLLSYFVLDVPIQGNLALLIAVSVLFIFLALSLGLLISTLVQTQVT
                     AVLISGIGLMMPTLILSGMIFPIDNMPEPLQWASSVVPARWYIAAVKKVMIEGLGFTH
                     VAKEVGILAGMVVFLIGASIAKIKNRL"
     gene            6182..7300
                     /locus_tag="PLI30_02265"
     CDS             6182..7300
                     /locus_tag="PLI30_02265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005793393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOI78409.1"
                     /translation="MSQLKYLIEKEFKQISRNAIIPKMIVLYPVLVLLIFPWAINFEV
                     KNIQIHVVDNARSVYSQRLINKIDASAYFILTGVSTRYNDAISDIEKEKADIVLEIPA
                     SFDTDMVKERRAKVMISANAVNSTQGLLGNNYLTQIINDYSQELRTELFPASALSDAS
                     HIDAVTDYRYNKRLDYKTFMLPAFIVLMMTLICGILPSLNIVIEKETGTIQQINATPV
                     SKFNFILAKLIPYWIIGLVILTLSFLVTWVIYGLLPSGSFATLYISAVVFLLGITGLG
                     IIISNYSDTLQQSMFLVMFFILIIILLSGMFTPVSAMPGWAQAVAYANPLTYFIEIMR
                     LVYLKGSALPDLFKPLMWLTGFAVFFNTWAVLSYKKRG"
     gene            7355..7948
                     /locus_tag="PLI30_02270"
     CDS             7355..7948
                     /locus_tag="PLI30_02270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI78410.1"
                     /translation="MKQNISTIVILAVVLLTGAACNRQAKEAAGGIQQPPKTTVEKTE
                     QLSKNLHDKLMSSFSPNWEMEEPAATDYPPYYGGSFIDNDGKFVICVVGNPEQYRPVL
                     ASILGTDDFLTEPCTYSYREMMQVMDRLDQFLSNSSVPADHPFLTRFAGAGADVFENR
                     VVVQLTEMNDDAIQSFKKDISNSPAVKFEKGEAPVLM"
     gene            7973..8638
                     /gene="queC"
                     /locus_tag="PLI30_02275"
     CDS             7973..8638
                     /gene="queC"
                     /locus_tag="PLI30_02275"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019540739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HOI78411.1"
                     /translation="MTDYKNEDALVLFSGGQDSTTCLFWAKQTFRNVEALCFSYGQRH
                     SLEVEVARNIAEIAGVSFQLLDASVISHLSPNSLTDASIVMDEEQPAGSYPNTFVPGR
                     NMLFITFAAAVAYAKNIKHLVTGVSEADYSGYPDCRDTFIHSLNATINLAMDKHFVIH
                     TPLMHRDKAKVWALADDLGVFDIVKNETLTCYNGIKAGGCGHCPACKLRNRGLQEYLA
                     DRK"
     gene            8798..9346
                     /locus_tag="PLI30_02280"
     CDS             8798..9346
                     /locus_tag="PLI30_02280"
                     /inference="COORDINATES: protein motif:HMM:NF018745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1345 domain-containing protein"
                     /protein_id="HOI78412.1"
                     /translation="MIAWIIFSCVFLILSWMVIFLRQVDQIKKKAVEDDGSTIFVFFM
                     VIISSFTNIFIVLLLMMSKDQTFMENPFFVPLNVAGILLSWAMMHTIYVFHYAHEYYN
                     GTGIKGERFTGGLDFPSEKNPDYMDFAYFSFVMGSTFQVSDVSIVSKRIRRIALVHGV
                     LSFLFNTFVVALTINIIAGLMK"
     gene            9482..9949
                     /gene="queF"
                     /locus_tag="PLI30_02285"
     CDS             9482..9949
                     /gene="queF"
                     /locus_tag="PLI30_02285"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018709562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033739 - preQ1 synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preQ(1) synthase"
                     /protein_id="HOI78413.1"
                     /translation="MSQTENLSLLGKKTAYKQDYAPEVLEAFENKHQSNDYWVTFDCP
                     EFTSLCPITGQPDFATIRIDYIPDVKMVESKSLKIYLFSFRNHGAFHEDCVNIIMKDL
                     IGLMDPKYIEVTGVFTPRGGISIYPYVNYGRSGTRYERLAEERLFNHSFPLNT"
     gene            9954..10775
                     /locus_tag="PLI30_02290"
     CDS             9954..10775
                     /locus_tag="PLI30_02290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019540021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ThuA domain-containing protein"
                     /protein_id="HOI78414.1"
                     /translation="MKKLSRRKFIQASVALGAGSVLMPENVLAQGMILNAERESTVLK
                     GKKVLYVYGGWDGHEPRQSVDIFVPWLRAEGAEVIVSDKLDTYADKELMESLDLIVQV
                     VTMAKITQEQEKGLLEAVKAGCGFAGWHGGIGDSFRENTEYQFMVGGQWVAHPGGIID
                     YRVKITDHKDEVTRGLNDFDMHSEQYFVHVDPNVKVLATTTFTGEHSSWIDGCVVPVA
                     WKKYYGTGRIFYSSLGHVMSDFNVPQALEIQKRGIRWAAESKYRPKEQWVSPVYK"
     gene            complement(10780..11559)
                     /locus_tag="PLI30_02295"
     CDS             complement(10780..11559)
                     /locus_tag="PLI30_02295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005851178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 16 protein"
                     /protein_id="HOI78415.1"
                     /translation="MPALLLNLSACSEQKKSDPKNDKWTLVWEDDFKGKQLDTTQWSK
                     IPRGKSDWNNYMSDYDGLYVMKDGNLVLRGIQNHVLPNDTAPFLTGGVYTKDKRTFGL
                     GRLEIKAKLNPAKGAWPAFWMLPKEGKWPDGGEIDIMERLSHDKLIYQTVHSRYTQTD
                     SLRVNPPASSIVGMNPDTYNVYALEKYPDSLVFYVNGTRTKNYPRITTSQEGQFPFAD
                     QEFYLLLDMQLGGSWVGAVNPMELPVEMYIDWVRYYEPKKN"
     gene            complement(11709..12101)
                     /locus_tag="PLI30_02300"
     CDS             complement(11709..12101)
                     /locus_tag="PLI30_02300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006844387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HOI78416.1"
                     /translation="MKTKILVTFALSGLLFISCNSGTSKENKTEREHIEMTPGTVNEE
                     HAMLGVKGSCEMCKDRIEKAAKSVENVTFASWDMEKQELHLNFDPDKTSIETISKAIA
                     AAGHDTDKDKAPDEVYNALPACCKYREP"
     gene            complement(12137..>12394)
                     /locus_tag="PLI30_02305"
     CDS             complement(12137..>12394)
                     /locus_tag="PLI30_02305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005790344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-associated domain-containing
                     protein"
                     /protein_id="HOI78417.1"
                     /translation="HAMLAVQGLCEMCKDRIEKAAKSVIGVNTASWNLNTKQLHLDFD
                     PAKTSVDAVSKAIAKAGHDTDKYKADKATYDALPSCCKYRD"
BASE COUNT         3487 a         2673 c         2836 g         3398 t
ORIGIN      
        1 aatcattatg ctggtgacgt aactggcata gaggttctca ttgacctcca caataatacc
       61 gtctttaatc tcggaatcca aaataaggtc tttttcccct gtgtctgttt taatctttga
      121 attgtttcca aacgttattt ttatttttcg caataccccg gccgggaaaa actgatcgat
      181 aatctgtttt gattttccgt tcgaaagcgg aagaacgttg taaacccctc cctggaaatc
      241 aagggtcgtc catttttcat cgctggtggt gttgtcggta gtagagattt caattttctg
      301 aatgtctacg ttaaattccg atatcagtaa tacgggagca tccgtaagtt ttattctcag
      361 ccgggtggcg tttatgctcg gatcgttctt ggagcatccc gtaaaagaga ttgcaaaaag
      421 aaaaagggaa aatagggtta gaatagcctt catttttagt caatttaccg caaagataac
      481 gaataacgga aaaaagatac gagaaaaaaa ccaaaatgtg ttttaaacaa atgtttaaaa
      541 cacttgttta ataaatgggg attgtatact tttgcatcaa aaaaataaac ctgaaaatgg
      601 aagacttgac tacggaacag aaaatagcag cagctgcgag aaaaatattc acccaaaaag
      661 ggtatgcggc gaccaagacg cgggacatcg cggaggaagc cggaataaac ctggcattgg
      721 tcaattacta cttcggcagc aaggaaaatc tgttcaaaca ggttttgaag gaaaaatttg
      781 aagctctttt cggcttgatg atgcccgttt tatcggatag agatatttct ctggatgaga
      841 aaatcaggct tcttgtcaag aattacacga aattgttaat agaaaatgaa gagctgccca
      901 ttttcgtgct caacgaacta agaaataatg aaaatatatt taaccccatt ttacagcagg
      961 cgcggacaat atcccagcca accatagacg agcagcttca acataacggt tttgccattt
     1021 ccacacctga ctttatcatg aatatcgtca gcctgaccat ctttcccttt atcgccaaac
     1081 ccctgtttat cacttcagga atggtgaaag aagaagattt tttacaattt atcgaaaaca
     1141 gggagaaatt gatccctgaa tggatttttg ccatcttaaa acaatctcag caatgaaata
     1201 cagaagattt acattattta taccgctgtt ttttataccc gtgttcacag ttaatgcaca
     1261 atcccttact atcgaagatt gtcaggggat ggctcgcgaa aattatccag ccattgcacg
     1321 gtttaatgtc attgaaaata tcaggagttt taatttggaa aacgctaaca gagcctatta
     1381 cccgcaactt tccctgaatg caaaagcaac atggcaaagc gatgttacaa agattgatct
     1441 ggagctgcct tctaatttcc ctcctcttga gattcctatt cccgataaag accagtatca
     1501 ggtctatggc caattgaatc agcttatctg ggatggtgga aaggtttcag cgcaaaaaga
     1561 gatgatcgtc gccaatgccg aagtggagaa acaaaaactt gaaaccgaaa tctattcgct
     1621 tcaggaacgg gtcaatcagg ttttttttgg tattttactg cttaacgaac aattgacgca
     1681 acagggtatc ctggaaaaag agttgcagcg aaacttagag aaagtgcaaa gctacatgtt
     1741 aaacggtgtg gccaacgatg ccgatttaag cgccgtaaaa gtggagcaac taaaaacaaa
     1801 ccagcaacgc attcaaatgg aatcggcgtt ggacagttat atcaagatcc tttctgtttt
     1861 aaccggacac cgtatagacc cgaaaaccgt ttttgtaaaa ccaccggtag ctgaagtacc
     1921 tgatcatgtg gttattgacc gtcccgaact gcggttattt acggctcagg aaaacctgct
     1981 gaattcacaa aaaacagccc tgaccgccag gaacaggcct gttatcagtg cgtttgccca
     2041 gggcggatac ggaaagccgg cattgaacat gtttgaaaat aaatttaaac cgtatgtgat
     2101 tggaggagtg agtttctcgt ggaatctcgg cagcctgtat acctacaaac aagagaaaaa
     2161 aagcattgaa cagcaaaaac tgacgctcga ttcagagcgt gagacctttc tctataacct
     2221 gaacgtgcag cttccgcaac agcaaaacga gataaaaaaa taccgccata ccatgcagga
     2281 tgatgacgag attatccggc tgagaaagca aataaaagac gctgcggaag caaaagtgga
     2341 gaacggaacg ctgacggttt ctgatttact gcaggagatc aacgctctgg aagttgccag
     2401 gcaggcaaaa tcgctgcacg aaattcaata cctgatgtcg gtttacaagt taaaattcac
     2461 aacgaattta aattgaaaat caatataaac agcaacaata tgaagccaac aaaacttttt
     2521 atttttccgg ttatactttt tcttcttgct tcctgttcag gcaatgacag cgactacgac
     2581 gccaccggat cgttcgaggc cacagaaatt attgtgtcgg cgcagggaag cggtgaaatt
     2641 ctgtcgctgg acattgcaga aggacaaaca ctacaagccg gagaagttgt ggggcagata
     2701 gacaccgtgc aactttttct acaaaaaatg agcttggagt caaacataca gggtgtccgg
     2761 gtgcaacgtc caaatatcgg tgcgcaaaca gctgccattg agaaacaaat tcaaacgttg
     2821 caacgggagc acacccgtac gcagaacctg attgaggcaa acgcagcaaa taaaaagcaa
     2881 ctggacgaca taaacgcgca aattgaagtt ttacaaaaac aattgtcggc ccaaacctcc
     2941 accttacaaa agagcagcga gaatatatac gcacaaagct ccgccttgca gattcaggta
     3001 gcgcaattgg atgatttgtt gaaaaaatgc accataaaaa gccctgcttc gggtgtagtg
     3061 ctcaacaaat acgccgaacg gggtgagctt gccggtacgg gatcaccgct tttcaaaatc
     3121 gccgacatcg acaatatgta cctacgtgcc tacatcacca gcgatcagct atctggcata
     3181 aaacttaacg attccgtgac cgtacgtgtg gatgccggag agggtgacat gaaatcatac
     3241 cccggtaccg tgtcgtggat atccggaaaa tcggaattca cgcccaaaac gattcaaacc
     3301 aaaaatgaac gggcaaacct ggtgtatgcg ataaaaattg ctgtaaagaa cgacggtttt
     3361 cttaaaattg gcatgtacgg agaagttaaa ttccggaacc tggagggaag gccatgatta
     3421 cggcaacaaa tttgcataaa acgtacggga aggtaaccgc gttaaacgac ctttcctttt
     3481 cggtaaacga cggagaaatc tttggtgtga tcgggcccga cggttcgggg aaaaccaccc
     3541 tttttcgcat tctagcctca ttgttgttgc ctgatagcgg atctgccgga ataaatggat
     3601 gggacttagt caaagatttc cggaaaattc gttgcataat cggatatatg ccggggaggt
     3661 tttcgcttta tccggatttg agcgtggaag aaaacctcaa gttttttgcc accgttttca
     3721 ataccaccct ggaggagaac tacccgctta tcaaggatat ttaccaacag attgaacctt
     3781 ttaagaaacg ccgggcggga gcattgtcgg gcgggatgaa acagaaactt gctctcagtt
     3841 gcgcgttgat acacaaaccc ggagtactga ttcttgacga acccacaacc ggtgtggacc
     3901 ctgtttcccg gaaagaattc tgggatatgc tggagcggtt gaaatccgaa gggataaccg
     3961 ttttggtttc cacggcatat atggacgagg caagcctatg tgaccgcatt gcgttaatgc
     4021 gcgaaggaag ctttatcgct accgatacgc cgcaaaacat cataaaccgt tatacagaaa
     4081 ccttgtggac ggtacaaact gaaaatttat cggcatcgtt gcacgattta cgggcgcatc
     4141 caaaggtgaa aacctgcttt gcgttcggaa atgaacacca tgttaccgtt cagccggatt
     4201 taccggtcgc cgggcttcaa tactatctaa aagaaaaagg gtattcaaaa gtgcagatca
     4261 atgttacgac gccgactgtg gaggattgtt tcatggcgtt gacaagtgaa acgtgaagaa
     4321 tggaaagtga tattgtaata aatgtctcgg gattgacgaa aaagtttggc aacttcaccg
     4381 ctgttgaccg tatctccttc gatgtccgca aaggggaagt atttggtttt ctgggtgcta
     4441 acggcgctgg gaagaccact gccatgcaga tgctgtgcgg gatcagcaag cctacatcag
     4501 gaaaagggac ggtggccgga tacgatatcc tgaaagaaaa cgagaagata aaacgcagca
     4561 tcggatatat gagtcagcgg ttttcgttgt acgaagacct gaaagtatgg gagaatatac
     4621 gcctttttgg tgggatttac gggttgacag acaaagagat aaaagaaaaa acggatgaat
     4681 tgcttcaccg gttgaggttc gaaagtgaac gaaacacttt ggttaaatcc ctgccgttgg
     4741 gttggaagca gaaactcgct ttttcggtag ctgtttttca cacgcctcaa attgtttttc
     4801 ttgatgaacc taccggcgga gtcgatccgg ccatgcggcg ccagttctgg gaaatgattt
     4861 acgaagcttc cgaccgtggg atcacggttt ttgtaaccac gcactacatg gatgaagccg
     4921 aatattgtca ccgagtatcg ataatggtcg acggaagaat tgaaacgttg gacagccccg
     4981 gcaacctcaa ggaaattcat caggcggaaa gcatggatga ggtattccgg aagttggcca
     5041 gaaaagcgac aagaatggat aatggataat agacaattga tatgaagcaa tttttctctt
     5101 tcgtacataa ggaattctac cacatactcc gtgacgggcg caccatgctg atccttctgg
     5161 gaatgccggt tgtgcagatt ttgttgttcg gattcgctat caatatggaa gtacaacata
     5221 tccgtaccgt ggtcttcgat ccggcacaag atgccgccac acgcgatatt accgaacgcc
     5281 ttatggcgaa tccctatttc catatgcagg gatatgtgta ctcgcccgat gagataaaca
     5341 agttgctgca acgaggggaa acggatgtgg ccatcgtgta cgaacaaaat ttcaacgaga
     5401 accttgtcca ttcaaagaaa gctcaactgc agatcattgc cgacgcatcc aatccaaata
     5461 cgggaaccat tgtcgcgaac tacgtgacgg caatcgttag cgaatatcgc ccccacattc
     5521 cttatcggat tgaagtggag aataaactgc tttacaatcc cgaaatgaaa tcggcataca
     5581 cttttgtgcc cggaatcatg ggattggtgc tgatgataat ctgtgccatc atgacctcga
     5641 tctccatcgt ccgggaaaag gagcgcggta cgatggaagt actcctggca tcgccgctaa
     5701 aaccaggaac ggtactgatt tctaaaacgg tcccttactt ggcactctcg ttcatcaacc
     5761 tgattaccat cctgttgttg tcttattttg tgttggatgt tcctatccag ggaaatttgg
     5821 ccctgctgat tgccgtatcg gttcttttca ttttcctggc gctctctctg ggtttgctta
     5881 tttctaccct cgtacaaaca caggtaaccg ctgtgcttat ctcgggaata ggactgatga
     5941 tgcctacttt gatcctgtca ggaatgattt tccccattga caatatgcct gaaccgttgc
     6001 aatgggcatc atcggttgta cctgcacgtt ggtacattgc tgcggtgaaa aaggtgatga
     6061 tcgaaggact gggatttacc catgtggcaa aagaagtagg tatccttgcc gggatggttg
     6121 tttttcttat cggggcaagc attgcgaaaa tcaaaaaccg actttaaaca attaaaacaa
     6181 catgagtcaa cttaaatacc tcattgagaa agagttcaag cagatcagca ggaatgcgat
     6241 tatacccaag atgatcgtgc tgtatccggt tttggtactg ttgatctttc cgtgggcgat
     6301 taattttgaa gtgaagaaca tacaaatcca cgtggtggac aatgccagaa gtgtctactc
     6361 ccaacggttg ataaacaaaa tagatgcatc agcttatttt attcttacgg gtgtatccac
     6421 ccgttacaac gatgccatta gcgatataga gaaagaaaag gcggatattg tcttggaaat
     6481 tcccgcttca ttcgacaccg atatggtgaa ggagcgacgt gctaaggtaa tgatttcagc
     6541 aaacgctgta aacagtacac aggggttgtt ggggaacaat tacctgacgc agattatcaa
     6601 tgattattcg caggaacttc gtactgaact ctttccggca tccgcactgt cggatgcatc
     6661 gcacattgat gccgtaaccg attaccgtta caacaaacgc ctcgattaca aaacctttat
     6721 gctgccggcg tttattgtgc tcatgatgac gctgatatgc ggtattttgc cttcactgaa
     6781 catcgtgatt gaaaaagaga ccggcaccat ccagcagatc aacgccacgc cggtgagtaa
     6841 atttaatttt attctggcaa agcttattcc gtactggatt atcgggcttg ttatcctgac
     6901 cctgtcgttc ttggtcacgt gggtgattta cggattgctt ccgtcgggaa gttttgccac
     6961 gctgtatatc tccgccgttg ttttcctgct ggggattacc ggattgggaa tcatcatttc
     7021 caactattcc gacacgttac agcagtcgat gtttctggtt atgtttttta ttctgatcat
     7081 tatcctgctc agcgggatgt tcacccctgt ttccgccatg cccgggtggg cacaggctgt
     7141 cgcttatgcc aatcccctca cctacttcat cgagatcatg cggctcgtat atctgaaggg
     7201 cagtgcattg cccgatcttt ttaagccgtt gatgtggcta accgggtttg cggtgttctt
     7261 caatacgtgg gcggtattga gttataaaaa acgtggctga ataattgaga ataggctatt
     7321 gaaacaaaag tgcaagaaaa gtgttattaa aaatatgaaa caaaacattt ccacaatcgt
     7381 tattcttgcc gtagtcctgt taacgggagc ggcctgtaac cgacaagcga aagaagctgc
     7441 cggcggtatt caacagcctc ctaagacaac tgttgaaaaa acggagcaac tttcgaaaaa
     7501 ccttcacgac aaattaatgt cgtccttcag ccccaactgg gaaatggagg aaccggcagc
     7561 aaccgactac cctccatact acggtggctc ttttatcgac aacgatggaa aatttgtgat
     7621 ctgtgtggtg gggaatccgg aacagtaccg tccggtatta gcctcgattc ttggaactga
     7681 tgattttctg actgaaccct gtacctattc ttaccgtgaa atgatgcagg tgatggatcg
     7741 tctcgaccag ttcctcagca attcgtccgt cccggctgac catccctttc taacccgttt
     7801 tgccggtgcc ggtgccgacg tttttgagaa cagggtggtt gttcagttga cggaaatgaa
     7861 tgatgacgcc atacaatcct tcaaaaaaga catttccaat tctccggccg ttaaatttga
     7921 aaagggagag gcgccggttt tgatgtaaga atgaaaagaa accctactaa aaatgacaga
     7981 ttacaaaaat gaagatgcac ttgtcctttt ttcaggagga caagattcta ccacctgcct
     8041 gttttgggca aaacaaactt ttagaaacgt tgaagcgttg tgcttttcct acggacaacg
     8101 gcattcgctg gaagtagagg ttgcccggaa cattgccgaa atagccggtg tgtcgtttca
     8161 gctgctcgat gcttccgtta tctcccacct atcgcccaat tcgttaacag acgcgtccat
     8221 tgttatggat gaagaacaac ctgccggttc atatcccaat acgtttgtgc cgggacgaaa
     8281 catgttattt atcacatttg cggcagctgt cgcttatgca aaaaacatca agcatctggt
     8341 aacaggcgtt tccgaggctg attacagcgg ataccccgat tgcagggaca cgtttatcca
     8401 ttcgttaaac gcaacgataa accttgctat ggataagcat tttgtcatcc acaccccact
     8461 gatgcaccgg gataaagcaa aagtatgggc acttgccgac gatctagggg tctttgatat
     8521 cgtaaaaaat gaaacactta cttgctacaa cggaattaaa gccggggggt gcggacactg
     8581 ccccgcatgt aagttacgca accggggatt acaggaatat cttgcggata gaaagtaaaa
     8641 cttaccgtgt acacaaaaat gcgccatgaa accagcctgt aaattcgtaa gttttttctt
     8701 ggaaattact catattactc ggtttttgat cagtttgttc attaccggtg gtgccttttt
     8761 tctgatagaa agagaggtcc ccgttctttt tcgcttaatg attgcctgga ttattttttc
     8821 gtgtgttttt ctaatcctca gttggatggt tatctttttg cgtcaggtag atcagattaa
     8881 gaaaaaagcg gtggaagatg acgggagtac aatatttgtg ttcttcatgg tgatcatctc
     8941 ttcttttacc aatattttta tcgttctgtt gttgatgatg tccaaggatc aaacattcat
     9001 ggaaaaccct tttttcgttc ctcttaatgt ggcaggtatc cttttgagtt gggcgatgat
     9061 gcatacgatt tatgtctttc attacgctca tgaatattac aatggaacgg gaattaaagg
     9121 cgaacgattt acgggaggtc tggactttcc ttcagagaaa aaccccgatt atatggactt
     9181 tgcgtatttc tcttttgtga tgggctctac ctttcaggtt tccgatgttt ctattgtttc
     9241 gaagagaata cgccgtattg cactggtgca tggagtcctg tcttttttgt tcaacacttt
     9301 tgttgtggct cttaccatta acattattgc gggactcatg aagtgaccgt attatcagaa
     9361 acgcttaaat aataggagaa cggcggatcg gattttctct tctacagaaa aacaggagaa
     9421 tattttattt gtattttttt tcgtattata tttgaagaat aatcgactta caattgaaat
     9481 tatgtctcaa accgaaaact taagccttct cggcaaaaaa acagcataca agcaagatta
     9541 cgctcccgag gtattggaag cttttgaaaa caagcaccaa agtaacgatt actgggttac
     9601 ctttgattgt cccgagttca cgagtttatg tcccataaca ggacaacccg acttcgccac
     9661 catacggatc gattatattc ccgacgtaaa aatggtagag agcaaatcgt taaagatcta
     9721 tctgttcagt tttcgcaatc acggtgcttt tcacgaggat tgtgtcaaca tcattatgaa
     9781 agacctcatc gggttgatgg atccgaagta tattgaagtt accggagttt ttactccccg
     9841 cggcggcatc agcatttatc cgtatgtcaa ctacggccgt tccggtaccc gatacgagag
     9901 attggctgag gaaagactgt ttaaccattc gtttccccta aatacctgaa aatatgaaaa
     9961 agttatcgcg tcgcaaattt atccaggcat ccgttgcgct gggagccgga tctgttttaa
    10021 tgcctgagaa tgtacttgca cagggaatga tcctcaatgc ggagagggag agcacggttt
    10081 taaaaggcaa gaaagttttg tatgtttatg ggggctggga tggccatgaa ccccggcagt
    10141 ccgtcgacat cttcgtgccg tggctccgag ctgaaggtgc ggaggtaatt gtttccgata
    10201 aactggatac gtatgccgac aaggaactga tggagtccct tgatcttatc gttcaggttg
    10261 tcacgatggc caaaataacg caggagcagg aaaaagggct gctggaagct gttaaggcag
    10321 gatgcggttt tgccggatgg cacggaggaa tcggggattc tttccgggaa aatacggaat
    10381 atcagtttat ggttggcgga caatgggtgg ctcatcccgg cggaattatc gattaccggg
    10441 tgaagattac cgatcataag gatgaagtta cccggggatt gaacgatttt gacatgcact
    10501 ccgaacagta tttcgtacac gtcgatccca acgtgaaagt gcttgccaca acgactttta
    10561 ctggagagca ttcctcatgg attgatggat gtgttgttcc tgttgcgtgg aaaaagtact
    10621 acgggaccgg acgaattttt tactcttccc tggggcacgt aatgagtgac tttaacgttc
    10681 cgcaggccct ggagattcaa aagaggggaa tccgatgggc ggccgaaagc aaataccggc
    10741 cgaaagaaca atgggtcagt cccgtgtata agtaacggct taatttttct taggctcgta
    10801 atagcgtacc cagtcgatat acatctctac gggtagctcc atcggattaa ctgctcctac
    10861 ccaactgccg cccagctgca tgtcgagcaa cagatagaac tcctggtcag caaatggaaa
    10921 ttgcccttcc tgagaagttg taatccgcgg ataatttttt gtccgggtgc cgttcacata
    10981 gaaaaccagg ctgtccgggt atttttcaag ggcgtatacg ttgtaggtat cgggattcat
    11041 acccacaatg gaactggctg gcggattaac ccgtaaactg tcggtttgtg tatacctgga
    11101 atgtacggtt tgataaataa gcttgtcgtg gcttaacctt tccataatgt ctatttcacc
    11161 tccatcgggc catttccctt ctttcgggag catccagaat gcaggccagg cacctttagc
    11221 aggatttaat tttgccttta tctccaaacg acccagtccg aatgtccgtt tgtctttggt
    11281 ataaaccccg cccgtcagaa aaggagccgt atcgttgggt aaaacgtggt tctggatacc
    11341 cctcagcacc aggttaccgt ctttcataac atacagtccg tcgtaatcgc tcatgtaatt
    11401 gttccaatcg ctttttcccc ggggaatttt agaccattgg gtagtatcca gttgtttccc
    11461 tttgaaatcg tcttcccaga ccagtgtcca tttatcattt ttcggatcgc tttttttttg
    11521 ttccgaacaa gcggaaagat tcaacaatag ggccggcatg agaaccagca aaaaagagtt
    11581 tttcattatt tcgtgatttt aattgtctac aaatgtaaga aaaataaaac agttaaccgg
    11641 aaaccaaaac acccgatttt gcagcgtttt gcaaaaccgg gtgtttttac aacgattaat
    11701 cccgtttctc acggttctct gtatttgcaa caagcgggca aagcgttata gacctcgtca
    11761 ggtgctttgt ccttgtcggt atcgtgtcca gctgcggcaa tagccttact gatagtctca
    11821 atactggttt tgtcgggatc aaaattcaga tggagttctt gcttttccat gtcccagctt
    11881 gcaaaagtga cgttttccac actctttgct gccttctcga tacgatcctt gcacatttca
    11941 caacttccct ttacgccaag catggcatgc tcttcgttga ccgttccggg tgtcatctct
    12001 atgtgctctc tctccgtttt attttctttg gaggttcctg aattgcagga aatgaacaat
    12061 aatccgctca gtgcaaatgt cactaaaatt tttgttttca tttttgtttt cactttttgg
    12121 tttgtaaatc tgattattaa tctctgtact tgcagcaaga aggtaacgca tcatatgtag
    12181 ccttgtctgc tttgtattta tcggtatcgt gcccggcttt ggcaatggct ttgctaacag
    12241 catcgaccga tgttttggcc ggatcaaaat caagatgtag ttgttttgta tttaaattcc
    12301 aactggcagt attaactcca ataactgatt tcgcagcttt ttctatacga tctttacaca
    12361 tttcacacaa tccctgcacc gccagcatcg catg
//
LOCUS       DAOSDR010000032        12197 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrimonas sp. isolate AMR_MDS_1826
            NODE_2015_length_12197_cov_4.037803, whole genome shotgun sequence.
ACCESSION   DAOSDR010000032 DAOSDR010000000
VERSION     DAOSDR010000032.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863355
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrimonas sp. (wastewater metagenome)
  ORGANISM  Petrimonas sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Dysgonomonadaceae; Petrimonas.
REFERENCE   1  (bases 1 to 12197)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12197)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:10:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,720
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 35
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12197
                     /organism="Petrimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2015_length_12197_cov_4.037803"
                     /isolate="AMR_MDS_1826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2023866"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..771)
                     /gene="tgt"
                     /locus_tag="PLI30_02310"
     CDS             complement(<1..771)
                     /gene="tgt"
                     /locus_tag="PLI30_02310"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006844006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - queuine tRNA-ribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HOI78418.1"
                     /translation="MKFELQHTDSSSQARAGLITTDHGQIKTPVFMPVGTVGSVKAVQ
                     ITELKDDIKAQIILGNTYHLYLRPGLDIMQLAGGLHKFNSWERPILTDSGGFQVFSLA
                     ENRKLTEEGAEFRSHIDGSKHFFSPEKVVDIQRIIGADIMMAFDECTPGDADYDYAKK
                     SLELTERWLKRCMDRFNETEGLYGYKQMLFPIVQGCVYPDLRRRAAENVASFEADGNA
                     IGGLAVGEPTEKMYEMVELVNEILPKDKPRYLMGVGTPA"
     gene            1248..3479
                     /locus_tag="PLI30_02315"
     CDS             1248..3479
                     /locus_tag="PLI30_02315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019538149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HOI78419.1"
                     /translation="MKIAYIGTYPPRQCGIGTFTENLCKSIALNVRQNTKTHASVIAI
                     NDSSSLQEYDYPPEVKFVVRQNYREDYINAAEQINLSNSRACILNHEFGIFGGESGLY
                     ILSLTDRLKIPLTVVFHTVLKEPSFLQKNIIRKINETAAKIVVMSNKAVELLIGIYDI
                     PPHKIEVIEHGVPDYQDVSHDAIKKEYNLSDRNVLLTFGFLGRNKGIETVIDALPAVV
                     QKHPSLLYIVLGATHPNILKYSGEEYRNYLNKLVRDRKLQDNVIFLKSFVTEDVLFEY
                     LRACDIYITPYNNKEQITSGTLAYAVGAGAAVLSTPYWHAEELLADGRGRLFDFKKPE
                     QLSDILNELLDHPEKMNALREKAYNYGIQLRWPTIGKRYLKLLNAVKDTPLVAKKPRK
                     SFIEVELLPEFTLDHVKRLTDDTGIVQHARYGIPNLKEGYCLDDNARALLMSLMAYGQ
                     NKDPFALDCMRIYLSYIQYMQTEQGTFRNFLSFRRDFLDKEGSEDSFGRTIWALGYLL
                     YASPNIAYREFGKELFDNSRKHFSRLEHLRGIADTVIGISYYLRSFPGDESLLQLMKE
                     LATKLADSYFENRDGQWQWFEPALTYDNAVLPLSLFHAAEISDDDVFKEIAVKTAAFL
                     EEATLSKGYLSPIGSYGWYTKGGERARFDQQAIDVMANVQLYFQAFNVTRKPDYIQKM
                     FQSFLWFLGENDLRVPLYDYETGGCCDGLKKDGVNRNQGAESTLAYLISHLTVLKAWE
                     CES"
     gene            3476..4504
                     /locus_tag="PLI30_02320"
     CDS             3476..4504
                     /locus_tag="PLI30_02320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019538150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HOI78420.1"
                     /translation="MKIAVLAPIAWRTPPRKYGPWELVASTITEELVRRGFDVTLFAT
                     GDSITGGKLSYACSQGYEEDKNVDPKVAECLHISSLMERAGEFDIIHNHFDFLPLTYS
                     RLIETPMITTVHGFSSPKIVPVYQRYNDINHYVSISYSNRNEHLSYIANVYNGINPRD
                     FTFCPAVGDYLLYFGRIHPDKGTREAIDIALKAKMKLIIAGYIQDNDYYKREVHPFVD
                     GRQIAYVGNADPVQRDKLLGQAYALLHPINFEEPFGLSVAESMFCGTPVIAFNKGSME
                     ELIEHGKSGFLVRNVDEALAVLPRIAELDRSDCRQHAQSRFSIERSVDDYVEVYKKVL
                     SLQRGCTS"
     gene            complement(4501..5967)
                     /locus_tag="PLI30_02325"
     CDS             complement(4501..5967)
                     /locus_tag="PLI30_02325"
                     /inference="COORDINATES: protein motif:HMM:NF015971.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 130 protein"
                     /protein_id="HOI78421.1"
                     /translation="MKIIINRLPNKFYSNYKQVIIRFLDLGEERTSALITKVLEMDES
                     DADKTLMYTLREFSKRHRNLTHKFIKHYSKAMEIVTNTLTEPEQISMVKKLLIGSYFS
                     MEYSISSAAFFNPSIVESPDQTGLSEGSKRIIVSFRATGEGHISSIVFQTGVINHRGE
                     LEFEKAGYHVDSAEVTKRHIYDKKSFHAKLHEMGVDELVIKPVMDSLTDKFIYGELLK
                     SVYEYLANNSNLEEEVQRSVKQIIWLADSHYTIHFSRDTHISERVIFPTSYTERKGIE
                     DARFVKFTDDDGETTYYATYSAYDGYAALQKLIVTKDFYTFEIKPLHGDGTQNKNLAL
                     FPRKIKGKYAMLSRIDGISSYIAFSDNINVWPAPVKIQEPKYSWEFIQIGNCGAPIET
                     SEGWLVITHGVGPMRQYSLGATLLDLDDPTKEIGRLRDPLLVPNDEEREGYVPNVVYS
                     CGSIIHNDELIVPYGLSDTGSGFMTVNVRKLLNKILNN"
     gene            6182..7054
                     /gene="lipA"
                     /locus_tag="PLI30_02330"
     CDS             6182..7054
                     /gene="lipA"
                     /locus_tag="PLI30_02330"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007571551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016992 - lipoate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HOI78422.1"
                     /translation="MNHSKEKCYIMMQRKPDWLKIELPQGKQYLDIKEVIARNKLHTI
                     CVSGKCPNLAECWGNGTATFMILGEICTRSCGFCATKTGKPLPVDWDELDRLADTISK
                     MNLKHCVITSVDRDDLRDYGAEFWAETIRYVKKKNPGITLETLIPDFLGKAELIHKVI
                     DAGPEIISHNMETVRRLTPLVRSRAKYDISLKTIETVAKSNKSKPKSGMMVGLGETSE
                     EVVQTMDDLRAVGCKVLTIGQYLQPTRKHLPVSEFISPDQFKEYKRIGLEKGFEFVES
                     GPLVRSSYRAERHV"
     gene            7074..7790
                     /gene="lipB"
                     /locus_tag="PLI30_02335"
     CDS             7074..7790
                     /gene="lipB"
                     /locus_tag="PLI30_02335"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013611155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033819 - lipoyl(octanoyl) transferase
                     activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HOI78423.1"
                     /translation="MDNKKVSFQNLGRIRYKKAWDLQEELFQSVVDAKVHHTLPKPQY
                     LLFCEHEPVYTLGKSGNRQNLLIAEQVCRSKNIDYYPIDRGGDITYHGPGQLVAYPII
                     DMEEFHVGVKAYVHKLEEVVIQTLRPYGIAAEKDEKAMGVWIDAQRPAKARKICAIGV
                     RTSRFVTMHGLALNVNTDLAYFNYINPCGFTDRGVTSMQKELGREVDFEEVTYRMKAA
                     FSDVFGMKVVEELCSPTPSF"
     gene            complement(7905..8207)
                     /locus_tag="PLI30_02340"
     CDS             complement(7905..8207)
                     /locus_tag="PLI30_02340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI78424.1"
                     /translation="MSILLVSLFFVACDKKDQPDPKLTLTPSTVEVKVTEDTTVKVSG
                     GTAPFTVVEADKTIATATVTNSDVKVTGVKEGQTTIAVTDKNKVKGTITVKVVKKS"
     gene            8715..9710
                     /locus_tag="PLI30_02345"
     CDS             8715..9710
                     /locus_tag="PLI30_02345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008761573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HOI78425.1"
                     /translation="MNQVVDIKELNERIERKSAFTQTLFTGMDKVIVGQKHLVESLLI
                     GLLSDGHILLEGVPGLAKTLAIKSLAQLIEAKYNRVQFTPDLLPADVVGTMIYSPRNE
                     EFQVKHGPVFANFVLADEINRAPAKVQSALLEAMQERQVTIGEKTYKLPNPFLVMATQ
                     NPIEQEGTYPLPEAQVDRFMLKVVIGYPTKEEEKRIIRQNIFEPVAIDKPVVSTEEIL
                     EARKVVRDVYIDEKIERYIIDIVFATRFPDQFGLKGMKEMIGFGASPRASINLALAAR
                     TFAFIKRRGYVIPEDIRAVCHDVLRHRIGLTYEAEANNLTSEEIVSEILNKVEVP"
     gene            9792..10661
                     /locus_tag="PLI30_02350"
     CDS             9792..10661
                     /locus_tag="PLI30_02350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019538360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HOI78426.1"
                     /translation="METSELLKKVRHIEIKARGLSRNIFAGQYHSAFKGRGMAFSEVR
                     EYQYGDDMRDIDWNVTARYNRPYVKIFEEERELTVMLLIDVSESLGFGSSEKIKRDTV
                     AEIAATLAFSAIQNNDKIGVIFFSDKVEKFIPPKKGRKHILFIIREILGFEPESAGTD
                     INLALRYMTNAIKKRCTTFLISDFIDTGDYKPALRIANRKHDIVAIQVYDKLSTRLPS
                     VGLMKVKDPESGGEMWIDTSSGNVRNHYAKWWLDRQAEMMLTFKQSSVDNVSVSTEDD
                     YAKALLLLFQRRR"
     gene            10695..11732
                     /locus_tag="PLI30_02355"
     CDS             10695..11732
                     /locus_tag="PLI30_02355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006799774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI78427.1"
                     /translation="MGKKVLHILLPLFLLISITHRSFAQRASVQASIQPAEIQIGEQA
                     LINLKVIAPKDKTIQFPVYQKEIIPGLEVLTMLPPDTVIENNVATMNFKYVVTSFDST
                     LYYVPHMPVFDGTDTIYSNSFGLKVTSPVLKDSTVAYLEKMNTGQTDSIDFHQLQLND
                     IKPVQKPAFVWTDYLWILWIVLGLILLLLLIGLIIVLVLRKKNKGYFFTPPQVLPPHV
                     RALQELDKLRAGKIWQQGREKEFYTKITDILRVYMFERYRINAMEMTSGEILVEIRKR
                     SEEDSIYNNLVQILSVADMVKFAKHKPYMDENDLSLMNAYFFVNQTREPDPLPDKKEQ
                     EKLKDEIDKEK"
     gene            11777..>12197
                     /locus_tag="PLI30_02360"
     CDS             11777..>12197
                     /locus_tag="PLI30_02360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007656551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HOI78428.1"
                     /translation="MQFANPTYLYLLLILAPLIAWYIVRMSKTQASFKLASTHAFSSK
                     NRGVKTYLRHLPFALRMMAVALIIVVVARPQAVDSWEESETQGIDIVLALDVSGSMLA
                     QDLQPNRLEAAKKVAAEFITDRKNDNIGLVIFAGESFT"
BASE COUNT         3399 a         2660 c         2907 g         3231 t
ORIGIN      
        1 ggccggagta ccaactccca tcagatagcg tggtttgtcc ttaggcagga tctcgttgac
       61 cagttccacc atttcgtaca ttttttccgt gggctcgccc acggccagtc cgccaatggc
      121 atttccatcc gcttcaaacg aagccacatt ttctgctgcg cggcgacgaa ggtcgggata
      181 cacacatcct tgcacaatgg gaaacagcat ttgcttgtat ccgtacaggc cttcggtttc
      241 gttgaacctg tccatgcatc ttttcagcca gcgctccgtg agctcgagcg actttttcgc
      301 atagtcgtaa tcggcatcgc cgggtgtgca ttcgtcaaaa gccatcatga tgtccgctcc
      361 gattatacgc tggatgtcta ccaccttctc cggagagaaa aagtgttttg aaccgtcgat
      421 atgcgagcga aattcagccc cctcttctgt caacttccgg ttttcggcaa gtgaaaatac
      481 ctgaaagcca ccgctatctg tcagaatggg cctctcccag ctgttgaact tatgcaatcc
      541 tccggcaagt tgcataatat ccaatcccgg ccgcaggtaa aggtggtacg tgtttcccag
      601 aataatttgc gctttgatgt cgtctttcaa ctcagttatt tgcacggcct tcacgcttcc
      661 cacggtacct acgggcataa atacgggtgt tttaatctgt ccgtggtcag ttgtaatcaa
      721 tcctgcccgc gcttgcgagg aggaatcggt atgttgcagt tcaaatttca aaacgataca
      781 attcttttgg tccaaccggg ttttgtgtga aaaccgtgct tactactgcc ggacgctgac
      841 agcgaataag tccttaatca cagaataccc ctggtctctt tttattactg gtctcttttt
      901 attaattgaa cgcaaagata agaattctgc tctcacaatc ttagcagttg tggattaaaa
      961 atagttagag agcattttgg atgagtcttc atccgatggc aagatctaaa tttttctgcc
     1021 ttattcttgc aaattcgggc aaaaatgtta tctttgtaac cacctggacc gaatctttct
     1081 tctgcagcgc gtttttattt acaacaacaa aacctgatgg aaatctattc tccatttaga
     1141 aattagtacc gggttgtcaa aagatagaca tcctggtaat tgcttcttcg attctccgta
     1201 aatgagcaaa ttcaatcaat cgtaacaatt aataaaacaa caaaaagatg aaaattgcat
     1261 acattggaac atacccgccc agacaatgcg gaataggtac attcacagag aacctgtgta
     1321 aatccatagc tttgaatgtg cggcagaata caaaaactca cgccagcgta atagctatta
     1381 acgattctag ctcgttgcag gaatatgatt atccgcctga agttaagttt gttgtccgtc
     1441 agaactaccg tgaagactat atcaatgcag cggaacagat caacttaagc aattcccggg
     1501 cttgtatcct taatcacgaa tttggaatct ttggagggga gtcggggttg tatatccttt
     1561 cccttactga ccggttgaaa attccactta ccgtagtttt tcatactgtc ctgaaagaac
     1621 cctcttttct gcaaaaaaac atcatccgga aaatcaatga aacagctgcc aaaatagtgg
     1681 tgatgagcaa taaggcggta gagttgctga tcggtattta cgatatacct ccacacaaaa
     1741 ttgaagttat tgaacatggt gtccctgact atcaggatgt ttcgcacgat gcgattaaaa
     1801 aagagtacaa tttgagcgac aggaacgtgt tgcttacgtt tggtttttta ggacggaaca
     1861 aagggataga gaccgtgatt gacgcgttgc ccgcagtagt gcaaaaacat ccgtcattgc
     1921 tttacattgt tctgggagcc acccatccca acatcttgaa gtattcgggc gaagagtacc
     1981 ggaattactt aaacaaattg gtgagggatc gtaaattgca ggataacgtg atttttctta
     2041 aatccttcgt tacggaagat gttctttttg aatacctgag ggcatgtgat atatacataa
     2101 ctccgtacaa caacaaagaa cagattacca gcgggacact ggcttacgcc gtaggagctg
     2161 gggctgctgt attgtctact ccatactggc atgccgagga attgttggcg gatgggcgcg
     2221 gacgcttgtt tgatttcaaa aagccggaac agttgtcgga tattttgaac gagttgcttg
     2281 atcatcccga aaagatgaac gcgttgaggg agaaagcgta taattacgga atacaattac
     2341 gctggccaac gatcgggaaa cgttatctca aactgcttaa cgcagtgaaa gatactccct
     2401 tggtggcaaa gaaaccccga aaatccttca tcgaagttga gcttttacca gaatttacct
     2461 tagaccacgt gaaaaggctg accgacgata caggcatcgt acaacatgcc cgttatggta
     2521 ttcctaatct aaaagagggc tattgcctgg acgacaacgc ccgtgcacta ttgatgtcgc
     2581 tgatggctta cggacaaaac aaagatccgt tcgcgttgga ttgtatgcgt atttatctca
     2641 gctatataca atacatgcag acagagcagg gtacattccg taacttcctc agttttaggc
     2701 gggattttct ggataaagag ggtagcgaag attctttcgg aagaacgatc tgggcgctcg
     2761 gatatcttct gtacgcttct ccaaacatcg cttacaggga gtttggcaaa gaattgtttg
     2821 acaattcgcg aaagcacttt tctagactcg aacatttgcg tggtattgca gataccgtca
     2881 taggaattag ctattatctg aggagctttc cgggcgatga atccctgctg caattgatga
     2941 aagaattggc caccaagctt gccgattctt atttcgagaa ccgggacggg caatggcaat
     3001 ggtttgagcc ggctcttacc tatgacaatg ccgtgttacc gttaagcctt ttccatgcgg
     3061 cagagatttc tgatgatgat gtttttaagg agattgccgt gaaaacagct gcgtttttag
     3121 aggaagccac gttgagtaaa ggatatcttt cgcccatagg gagttacggc tggtacacga
     3181 aaggtgggga acgggcgagg ttcgatcagc aggccatcga tgttatggca aatgtacagt
     3241 tgtattttca ggcatttaac gttaccagga aaccggatta tattcaaaaa atgtttcaat
     3301 ctttcctgtg gtttttgggt gaaaacgact tacgtgtgcc gctttatgat tacgaaacgg
     3361 gaggatgttg cgacgggctc aaaaaagatg gcgtgaatag gaatcaaggt gcagagagca
     3421 cattagccta tcttatttct cacctgaccg ttcttaaggc atgggagtgc gaatcatgaa
     3481 aatagccgtt cttgccccta ttgcctggag gacacctcct cgcaaatatg gaccctggga
     3541 gctggtcgct tcaaccatta ccgaagaact ggtgagaaga ggattcgatg tgacgctttt
     3601 cgcaaccggt gattccatta ccggagggaa attgtcgtat gcctgctctc aaggctacga
     3661 ggaagacaaa aatgtcgatc cgaaagtagc tgaatgcctg cacatcagct ccctgatgga
     3721 gcgggccggt gagtttgata tcattcacaa tcactttgat tttctccccc tgacctattc
     3781 aaggttgatt gaaactccaa tgataaccac cgtgcacggt ttttcatcac ccaagattgt
     3841 tcccgtatac caacggtaca acgacataaa tcactatgta tccatcagct actccaatag
     3901 aaatgaacac ctgagctata ttgccaatgt gtataatgga atcaatcccc gagattttac
     3961 gttctgtcct gctgtgggag attatctgct gtatttcggc cgcattcatc ccgacaaggg
     4021 tacgcgagaa gcgatagaca ttgctctaaa agcaaaaatg aaactgatca ttgcagggta
     4081 catccaggat aacgattact acaaaaggga ggtgcatcct tttgttgacg gccggcagat
     4141 agcctatgtg ggaaatgccg atcccgttca acgggataaa ttattgggcc aagcctatgc
     4201 gttgcttcat ccgattaact ttgaagaacc ttttgggtta agcgttgccg aaagtatgtt
     4261 ctgcggtaca ccggttatcg cttttaataa aggatcgatg gaggaactga tcgagcacgg
     4321 gaaaagtggt tttctggtcc gcaatgtgga tgaagccctg gcggtcttac cccggattgc
     4381 ggaattagac cgcagcgact gtcggcaaca tgcccagtca agattctcga tagaaagatc
     4441 cgtcgacgat tatgtggaag tgtacaagaa ggtgctatcg ttgcagcggg gttgcacctc
     4501 ttaattattc aaaatcttat ttaataattt acggacatta accgtcatga accccgagcc
     4561 ggtatctgac aatccgtagg gaacaataag ctcatcatta tggatgatgg acccgcagga
     4621 ataaactacg ttgggtacat atccctctct ttcttcgtcg ttagggacaa gaagggggtc
     4681 cctgagcctt ccgatttctt ttgtggggtc atccaaatcg agcagagtcg ctcctaggga
     4741 atactgacgc atcggcccta cgccatgcgt aattaccagc cagccttcgg aggtttcaat
     4801 cggggcgccg cagttcccga tctgaataaa ctcccaggag tatttcggtt cctgtatttt
     4861 gacaggtgcc ggccacacgt ttatgttgtc tgagaaggcg atatagctgc ttattccgtc
     4921 gattctggac aacatggcgt atttgccctt gatttttcgg gggaacagcg ccaggttttt
     4981 attctgtgtc ccgtcgccgt gaagtggttt tatttcaaac gtataaaaat ctttggtcac
     5041 gattagtttc tgtaaagccg catagccatc gtaggcactg tacgtggcat aataggttgt
     5101 ttcaccgtca tcatcggtaa acttcacaaa gcgggcatcc tcaatacctt tccgctcggt
     5161 ataggacgtg ggaaaaatta cgcgttccga tatgtgcgta tcgcgcgaga aatgtatggt
     5221 gtagtgcgaa tccgccaacc aaattatttg cttgacggaa cgctgtacct cctcttccag
     5281 gttgctgttg tttgccagat attcatatac cgatttcaat aactctccat agataaactt
     5341 gtctgtcaag gaatccatta ccggttttat gacgagttca tcaactccca tctcatgaag
     5401 cttagcgtga aagctttttt tatcgtagat gtgtcttttt gttacttcgg cactgtcaac
     5461 atggtatccc gctttttcaa actccagttc tccccggtga tttatcaccc ctgtttggaa
     5521 cacaatactt gatatgtgtc cttcgccggt tgcgcgaaaa ctgactataa ttcgttttga
     5581 tccttcactc aggccggttt gatctggaga ttcaacaatt gagggattga aaaaagccgc
     5641 agagctgata gaatattcca tggaaaaata ggagccgatc agcaatttct tcaccatgct
     5701 gatctgctcg ggttcagtga gtgtgttagt gacaatttcc atcgctttgc tatagtgctt
     5761 tatgaatttg tgtgtcaggt tacggtgccg ttttgagaac tctctcaacg tgtacatcaa
     5821 tgttttgtcc gcatcgcttt catccatttc caataccttt gttatcaaag cgcttgtccg
     5881 ctcctcaccc aaatccagaa aacggatgat tacttgtttg taattggaat aaaacttatt
     5941 gggtaagcgg ttgataataa ttttcatacg atgtagtatt taaaagaatt ggcaggggtt
     6001 attcttcgga ataggaccct gaaacaaact tacatcaata aaaagacata ttcaaatatc
     6061 aacgcataaa aattctccaa ccttgttaag aaaaagggag gataattgtc aaatttccgt
     6121 tttagatgaa acggtatggt gcatctgaaa ataatatgct atttttgtgg cacactttat
     6181 tattaatcac agcaaagaaa aatgttacat catgatgcaa agaaaacccg actggcttaa
     6241 aattgagctc cctcaaggga agcaatacct cgatataaag gaagttatcg caagaaataa
     6301 actccacacc atttgtgtga gcggcaagtg tcctaatctg gcggaatgtt ggggaaacgg
     6361 aacggcaaca tttatgattc tgggagaaat atgtacccgt tcctgtggct tctgtgctac
     6421 gaaaaccggc aaacccttac cggtagactg ggatgaactg gacaggctgg ccgacacaat
     6481 ttccaaaatg aacctgaaac attgtgtaat aaccagtgtt gacagagatg acctgaggga
     6541 ttatggcgcg gagttctggg cggaaaccat caggtacgta aagaaaaaga atccgggaat
     6601 taccctggaa acactgattc ctgattttct gggaaaagca gagcttattc ataaagtaat
     6661 cgatgccggc ccggagataa tctcgcacaa tatggaaact gtacgtaggc tcacaccgct
     6721 ggtgcgtagt cgcgctaaat atgacataag cctgaaaacc attgaaaccg tggcaaaaag
     6781 taacaaaagc aaaccaaaat cgggaatgat ggttggtttg ggagagacat ctgaggaggt
     6841 agtgcaaact atggatgacc tgagggctgt tggatgcaag gtgctgacga tcgggcaata
     6901 ccttcagcct acacgcaagc atcttccggt gagtgagttt atatcccccg accagttcaa
     6961 ggaatacaag aggataggac tggaaaaagg attcgagttt gtggaaagtg gcccgttggt
     7021 gcgttcttcc taccgggcag aaaggcacgt ataaacgttg gacttttcta cgtatggaca
     7081 acaaaaaagt aagctttcaa aatcttggcc gcattcgtta caaaaaggcc tgggatttgc
     7141 aggaagaact gtttcaatcg gtggtggatg caaaggttca tcacaccctg cccaaaccgc
     7201 aatacctgct tttttgcgaa cacgagccgg tgtataccct gggaaagagt ggcaacaggc
     7261 agaatcttct tattgccgaa caggtgtgta gaagcaaaaa tatcgattat taccccatcg
     7321 accggggtgg agacattact taccacggtc cgggacaact ggtagcgtac cccatcatag
     7381 atatggaaga gtttcatgtc ggggtaaagg catatgttca taaactggag gaggtggtca
     7441 ttcaaactct tcggccgtac ggaattgctg cggagaagga cgagaaagcc atgggggtct
     7501 ggatcgatgc gcaacgtcct gccaaagccc gtaagatctg tgccatcggt gtgcgtacca
     7561 gccggtttgt aaccatgcac ggcctggcgc tcaatgtaaa caccgatctg gcatacttca
     7621 actacatcaa tccctgcggg tttaccgaca gaggagtaac gtccatgcag aaggaactgg
     7681 gtagggaagt ggattttgag gaagttacgt acaggatgaa agcagcattc tcggatgtgt
     7741 ttggaatgaa ggtggttgag gaactatgta gtcctacgcc gtctttttga tggctagtca
     7801 ctctgcggga tatgcccgcc tggtggcgag tatcgggtaa aaaaacggca tgatgttcga
     7861 aaacatcatg ccgaaaacat atgtagatct gtttaattat accatcagct ctttttcact
     7921 acttttacgg taatggtacc ttttactttg ttcttgtccg taaccgcaat cgtggtttga
     7981 ccttccttca ctcccgtaac tttcacatca ctgttcgtta ccgtggcagt ggcgatggtt
     8041 ttgtcagctt ctacaactgt aaatggagcg gtgccgccgc ttaccttgac ggttgtatcc
     8101 tcagtaactt tcacttcaac cgttgaagga gttaatgtca gttttggatc gggctgatct
     8161 tttttgtcac aagccacaaa aaagagcgat acaagaagta tgctcattat agaacttagt
     8221 cttttcatac ttaaaacatt taaaatttga aattaattta atgattgttc aaaaacggcc
     8281 aaaattttag gctgccaaaa ttcgaattta tttcgatcgt tttaaacata gatgcggaaa
     8341 actgaaaaac gctgcatgga aatgctgtat ttaaccatga ttaacgttga aatgtgattg
     8401 tggaaaaggg atttgaaact ataaagccga gagtacgctc ctatttggca aacagataac
     8461 ccgtttgaaa aagataaaca agggacgaaa aagaaagaac cctcaatttc aaagcataac
     8521 cttaaacatt tttaactgcg aaatcaaccg taacgattta acatttccta tttttgtgtt
     8581 tcgaaagccg aaaggctcgt ttggggttaa cggatgttgt gaaaatagga cctgacttgt
     8641 tctgcttttg gctgacagct acacgatatt ttcagacatt tccttcagca ccgtaaattt
     8701 aaatagatta caatatgaat caagtggttg atatcaagga attaaacgag agaattgagc
     8761 ggaaaagcgc ctttacccaa accctgttta ccggaatgga taaagtgatt gtgggacaga
     8821 aacatttggt ggagtcgctt ttaatcggat tgttgtccga cggacatatc ctgcttgaag
     8881 gggtgcccgg gctggcaaag acattggcca taaaatccct ggcccaactg atcgaggcaa
     8941 agtacaaccg tgtccagttt acgcccgacc tgttgccggc cgacgtcgtt ggaaccatga
     9001 tatacagtcc ccgcaacgaa gagtttcagg tcaaacacgg tccggttttt gcgaattttg
     9061 tgttggcgga cgaaataaac cgtgcgcctg ccaaagtgca gagtgcgttg cttgaggcca
     9121 tgcaggaacg tcaggtcact atcggcgaaa aaacctataa gctgcccaat ccttttctgg
     9181 tgatggccac gcaaaacccg attgagcaag aagggaccta tccgttgccc gaagctcaag
     9241 tcgaccgttt catgctgaag gtggttatcg gttatccaac caaggaggaa gagaaacgca
     9301 tcattcgtca gaatattttt gagccggtag ccattgacaa gccggtagtc tctaccgaag
     9361 agattctgga agcacgtaaa gtagtgcgcg atgtttatat cgatgaaaag atcgaacgtt
     9421 acataatcga tattgtgttc gctacccgtt ttcccgacca gttcggctta aagggcatga
     9481 aagagatgat cggattcggt gcatcgccgc gtgcgtccat caacctggcg ctggctgccc
     9541 gcacatttgc ctttatcaag cgccgcggtt atgtgatccc cgaagatatc cgtgccgttt
     9601 gccacgatgt tctccgacac cgcatcggac tcacttacga ggcagaggct aacaacctga
     9661 catcggaaga aattgtcagc gaaatcttga acaaggtaga ggtaccctaa taataacggc
     9721 cgggaggcct gaagaatggg agagaggacg atgttgacag gtggcgcttg cgtctcaaaa
     9781 aaagggagta aatggaaacc agcgaattac taaagaaagt acggcatatt gaaataaaag
     9841 cacgggggtt gtcgcggaat attttcgcgg ggcaatacca ctcagccttt aaaggacgcg
     9901 gtatggcgtt ttccgaagtt cgcgagtacc agtacggcga cgatatgcgt gatattgact
     9961 ggaacgttac tgcccggtac aaccgtccgt acgtgaaaat tttcgaagaa gagcgtgagc
    10021 ttaccgtgat gctgcttatt gatgtgtcgg aaagcctggg ttttggatcg tcggagaaga
    10081 tcaagcgtga tacggtggcc gaaatcgcgg caaccttggc gttttcagcc atccaaaaca
    10141 acgataagat tggtgtcatc tttttttccg acaaggtgga aaaatttatc ccgcccaaga
    10201 agggaagaaa acacattctt tttattatcc gtgagattct gggctttgaa cccgaaagtg
    10261 ccggaaccga tatcaacctg gcgttgcgtt acatgaccaa tgccataaag aaacgttgta
    10321 ccacattttt aatctccgat tttattgaca cgggcgacta caagcctgcg ctgcgtattg
    10381 ccaaccgcaa acacgacata gtcgctatcc aggtatacga taaactcagc acacggttgc
    10441 cgtcagtggg gttaatgaag gtgaaagatc ccgaatccgg gggcgaaatg tggatagata
    10501 cctcatccgg aaacgtaagg aaccattatg cgaaatggtg gctggaccgt caggctgaga
    10561 tgatgctcac attcaagcag agcagcgtgg ataacgtctc cgtttcaacg gaagacgatt
    10621 atgcgaaagc gttattgctg ttgtttcaaa gaaggagata ggggaagaag ttggaattta
    10681 gaagaaatca agaagtggga aaaaaggttc tgcatatatt actgccgtta tttttgctga
    10741 taagcattac gcaccgttct tttgcgcaac gtgcttcggt gcaggcaagt attcagcccg
    10801 ccgaaattca gatcggtgag caggcgctta tcaacctgaa ggttatcgcc cccaaggaca
    10861 agaccatcca gtttcccgtt tatcagaaag aaatcatacc cggactggaa gtgttaacca
    10921 tgctcccgcc cgataccgtt attgagaaca acgtggccac catgaatttc aaatacgtgg
    10981 tcacgtcgtt cgactccacg ctgtattacg ttccccatat gcccgttttc gacgggaccg
    11041 acaccatcta ttccaactct ttcgggctga aggtgacatc gcctgtgctg aaggattcca
    11101 cagtagcata cctggaaaaa atgaatacgg gacaaaccga ttccattgat ttccaccaac
    11161 ttcagcttaa tgacataaag ccggttcaaa agccggcatt tgtatggacg gattatctct
    11221 ggatactgtg gatagtgctg ggtctcattt tgcttctgct gctgatcggc ctgattattg
    11281 tccttgtact ccggaaaaag aacaagggct atttcttcac cccgccgcaa gtgctgccgc
    11341 cgcacgtccg tgcccttcag gagctggata agctcagggc cgggaaaatc tggcaacagg
    11401 gccgtgaaaa agagttttac acgaagatta ccgatatcct gcgggtgtat atgtttgaac
    11461 gctacaggat aaatgccatg gagatgactt caggtgaaat tctcgtggaa ataagaaaaa
    11521 ggtcggaaga agattccatt tacaacaacc tggttcagat tctttcggta gcagatatgg
    11581 tgaagttcgc gaagcataaa ccctacatgg atgagaacga cttaagtttg atgaacgcct
    11641 atttctttgt caaccaaacg cgcgaacccg atccgcttcc cgataaaaaa gaacaggaaa
    11701 agctaaaaga cgagattgat aaagaaaaat agacaaaaaa gacgaaaagt ctgaaacata
    11761 aaggtcttac aagaatatgc aatttgccaa ccctacatat ctttatttgc tgctgatact
    11821 ggctccgctg atcgcgtggt acatcgtgcg catgtctaaa acacaggcgt ccttcaaact
    11881 ggcttccacc catgccttca gcagtaagaa caggggagtt aaaacgtacc tgcgccacct
    11941 gccgttcgcg ctaaggatga tggcagttgc gctgataatt gttgttgtgg cacgcccgca
    12001 ggcggtggat agctgggaag agtctgaaac gcagggaatt gatatagtgc tggctcttga
    12061 cgtgtcgggc tcgatgctgg cgcaggattt gcaacccaac cggctggaag ccgctaaaaa
    12121 agtggctgcc gaatttatca ccgaccggaa gaatgataac atcggattgg tgatttttgc
    12181 cggggaaagc tttacac
//
LOCUS       DAOSDR010000033        12123 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrimonas sp. isolate AMR_MDS_1826
            NODE_2035_length_12123_cov_3.996271, whole genome shotgun sequence.
ACCESSION   DAOSDR010000033 DAOSDR010000000
VERSION     DAOSDR010000033.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863355
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrimonas sp. (wastewater metagenome)
  ORGANISM  Petrimonas sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Dysgonomonadaceae; Petrimonas.
REFERENCE   1  (bases 1 to 12123)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12123)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:10:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,720
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 35
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12123
                     /organism="Petrimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2035_length_12123_cov_3.996271"
                     /isolate="AMR_MDS_1826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2023866"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..631
                     /locus_tag="PLI30_02365"
     CDS             <1..631
                     /locus_tag="PLI30_02365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019541268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HOI78429.1"
                     /translation="SITPEIIDSLKEFVGYDKIRIDSEGNVVKSDPRLQLNTSAISKG
                     YACDLVARLFDSYGIENYMIEIGGEIVTKGVNDKGVCWRIGVDKPIDDSTGMQHELQT
                     ILSLCDKSLATSGNYRNFYVKDGKKYAHTIDPQTGYPSEQDILGATVIAEDCMTADAY
                     ATAFMASGLEKSKEIARKIPGLHYYFIYVKPDSTLDIAYSEGFEQFFAN"
     gene            633..1181
                     /locus_tag="PLI30_02370"
     CDS             633..1181
                     /locus_tag="PLI30_02370"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: protein motif:HMM:TIGR00589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HOI78430.1"
                     /translation="MNNIVSIVPLNKKEVKDTVVSYFFYETDYGLAVIASTSKGICYV
                     GFGEKEPMLNDLKKRYSGTALQEQKAGLHKLALSFIKNERVSELPLHIAGTDFQLSVW
                     KALLQIPVGKLSSYKAIAKAVNNPKAVRAVGSAIGDNPVSYIIPCHRVIRSDGGLGGY
                     FWGLEIKKKMIEREAQTAEIEN"
     gene            1186..2214
                     /locus_tag="PLI30_02375"
     CDS             1186..2214
                     /locus_tag="PLI30_02375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI78431.1"
                     /translation="MLNVKQSMSLKKLFVAIFMMFSLLTPAQETDSVRYPTFKVDGTM
                     KNKFEYASETGSSRFSVRNSRMGVRGYLTPFFSYRGQIELSDNGNFKVLDLYGSFEPV
                     RGLSLSLGQTSIPIFNSYVVSPANLMFANRAFIGKYFISTRDIGFLADYNFKIDAVPA
                     SIEFGAYNGNTINDPVWREKLSYGGRLELGSMKGLRSTFKFYDYLNTPTVHYFFYGAD
                     LRYEGANWKVETEFMKRDNKEVDEDQMFSYYLQGAYAVPLKETYFFKNIVPAVRWDAI
                     DKHMNEKGFDVDRLTVGLGFGLTKKYFSSILRFDYEWYFINQELDILNLYEEMDSDKF
                     TVELLLTF"
     gene            2228..3613
                     /locus_tag="PLI30_02380"
     CDS             2228..3613
                     /locus_tag="PLI30_02380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006797897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1080 domain-containing protein"
                     /protein_id="HOI78432.1"
                     /translation="MAKKILSFLCVVSLILPGCTEQNKKKILFDGKDVTNWLTEGPVT
                     VSDSVVRMDGVGKMTLKNGMFTDFELLLTAKTVDNGKGEVRFHTDENGNGGYAVALNN
                     DLDHQQWWTKTGSLLSVRNLTKSIVKNNEWFNLRIRVEGKKIEVAVNDELLVDYIEPA
                     QPYRTPENRSQILSGGTFCLQSTEGIVEVKFIEVTPLKIEKTVIDSQLVQAIDESSDE
                     IIKLHQANFPVLDYHVHLKEDLTLELARSQSRKYGINYALAPNCGIGFPIQNDAQVLE
                     YFNGMKGQPFVQAMQGEGREWPATFSKEVRDLFDYVFTDAMTFTDRKGNRTRLWMPDE
                     VFIDDEQKYMDLIVENIVKVMDEPMDVYVNPNFLPDAMNDRYDLFWTDERQNKVIEAM
                     VRTHKVLEINHRYKIPNKSFIQKAKAAGLKFTFGTNNSNSDFGKMEYCIQMMKECGIT
                     AQDMYKPAMTE"
     gene            complement(3629..4852)
                     /locus_tag="PLI30_02385"
     CDS             complement(3629..4852)
                     /locus_tag="PLI30_02385"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005940163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003941 - L-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009063 - amino acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HOI78433.1"
                     /translation="MKSLKELYRIGNGPSSSHTMGPKKAAELFLERAPGAAKYVVTLY
                     GSLAATGKGHLTDTAILNVLEPHVPTTIEWLPRTFLPFHPNAMRYDALDATGNKLDSW
                     TVYSVGGGALSDGTTVVGLSEAAQKDIYPMTTIADIQAWCEQNGRTYWEYVEIHEDND
                     IWEHLRDVWQVMKAAVKSGLDNEGVLPGPLNLQRKAASYFIKAKGYKASLQSRGLVFA
                     YALAVSEENASGGKIVTAPTCGSCGVLPAILYYLEESRDFSETRILRALATAGLFGNV
                     VKQNASISGAEVGCQGEVGVACSMAAAAASQLFGGSPAQIEYAAEMGLEHHLGMTCDP
                     VCGLVQVPCIERNAYAAARALDANLYSSFTDGIHLVSFDRVVEVMKQTGHDLPSLYKE
                     TGEGGLARDYKRMKN"
     gene            complement(4872..7430)
                     /locus_tag="PLI30_02390"
     CDS             complement(4872..7430)
                     /locus_tag="PLI30_02390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015531291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 protein"
                     /protein_id="HOI78434.1"
                     /translation="MKKLSLWLLAFLPVFGYAQKNGETLWLNEGWTFSQTGKDTWYEA
                     QVPGSVQHDLIRHKVLPDPFYGTNERLVQWVEDENWDFKKTFTVSAEQLRHDDAVIFF
                     EGLDTQADVFLNGARILRSENMFVGYKISVKNILKEGENSLYIRFYSPVQSLMPARLT
                     AGYDYPAGNDHRDEKLSIYNRKAPYQFGWDWGIRIAQMGIWKPVSLVFYDKVRIDDFF
                     VKQTSVTAQSAKIENIVEVFSIAEKPVSATVTVEYAAAGEAKRSVSKQVTLHRGNNTV
                     FLELEIAYPKRWMPTGWGDQHLYDFTANVAAENRVITEKTEKIGLRSVRLVQEPDKYG
                     KSFYFEVNGIPLFAKGANYIPGEIFTTQQNEDYYTKLFDNIEAANMNFVRVWGGGIYE
                     NEEFYRQADKRGILVWQDFIFGCVPYPSDDRFLANVKDEVIYNLKRLRNRASLAFWCG
                     NNEVEEGLQHWGWDKQYPADIYNVWLEGYDKTFRELIPGLVNEFDGTRSYIHGSPYDS
                     NWGNPETFASSDVHDWGLWYGHLPFEGMAGRLPRFASEFGFQSFPEMKTIRSFSPENE
                     WSLESEVMKVHQKASTGNSLIKKYMDMYYHEPRNFEDFVYIGLVMQGNGMEESVEAMR
                     RGRPYCMGALYWQINDDWPVVSWSSIDYYGNWKAQHYRMRDVLAPLALGVEFKDNQLN
                     YYTLSDFLIDRNGLQLTVQVVDFNKGVVKQFKEKVNAKANSSNVVKTFNVEELLSEAD
                     KSNRMIRAWLTDSKGKKLSVKDHFFYWPNKLNLPQTTVQTSVKYADGKYTVTLTSKKL
                     AKDVFIEIPVMGAKFTDNFIDLLPGEKKVIEITSPELKASAKTPVTVRHIRETY"
     gene            complement(7449..8198)
                     /locus_tag="PLI30_02395"
     CDS             complement(7449..8198)
                     /locus_tag="PLI30_02395"
                     /inference="COORDINATES: protein motif:HMM:NF018178.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1080 domain-containing protein"
                     /protein_id="HOI78435.1"
                     /translation="MKNLFIPAVLLIFGIVAGCQNKKTDDGFVPIFDGKSLDGWKGDS
                     TYWRIENGILTGEITPETVLSRNSFIVWQDGAPENFELKLEYRISEDGNSGINYRSEE
                     IADLPHALRGYQFDIDGKNNYTGQNYEERRRTTLAYQGQTVVLNPLPDSLSSLPVSDL
                     VKNNAWTKAEIVELADVAELKSHIKNNDWNSCHLIVNGNKMKHILNGVLMSEVTDNDL
                     LNGKKQGLIGVQVHVGPPMKVEFRNIRLKTL"
     gene            8381..10585
                     /locus_tag="PLI30_02400"
     CDS             8381..10585
                     /locus_tag="PLI30_02400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019539161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HOI78436.1"
                     /translation="MKTIPTISFDDYVLLHRRLYGLFRQRWSLQKTGFLKRVWEDSLQ
                     RSVDEGSEDLLQGLLFDMKTAVILVDEIGLKYAVVCAVLMFRPLMKGYVTVDEIRKEF
                     GEEVAVIIRGLKKVNDLTEKKSRVESENYIKLLLSIAEDIRVVFILLAEQLQLMRDAK
                     KSTPENRLDLSVESTYLYAPLAHRLGLYTIKSELEDLSLKYTDRETYDFIARKLNESK
                     RSRDKYIEEFIHPLKERLDKTNLKYDIKGRTKSIHSINNKLKKQKIEFDNIYDLFAIR
                     IILDSPSEMEKAHCWQVYSIVTDMYQPNPKRLKDWLSIPKSNGYESLHITVMGPDSKW
                     VEVQIRTRRMDDIAERGLAAHWKYKGIKEESTLDDWLKSLRESLESRDVDIRDKLGDF
                     KLDLYDEEIFVFTPKGDLFKLPKGASVLDFAFAIHSKLGATCVSGKVNGKNVPIKHML
                     KSGDQVEINTSSHQSPKQDWLNYVVTSKAKTRIRQLLKEEAGKQVDIAKEMLSRRMKN
                     RKIDLDDPLLMQLIKKLKYKTVTDFYIDIAAEKLEVNWVIDRYLELGSKEFEHRDFLS
                     ATSADSFVLKPSSQEIKSSDELIIDQNLTGVDYRLAKCCNPIYGDEIFGFVSSQGIKI
                     HRTSCPNAHDLFSRFGYRILKARWSGKTSGTANYTSVLRIIGNDQLNIVANLMSIISK
                     EDGVQMRSISIDSNDGLFQGNIAVMLASTSMLEQLIKKLKAVKGVKSVSRLN"
     gene            complement(10643..11014)
                     /locus_tag="PLI30_02405"
     CDS             complement(10643..11014)
                     /locus_tag="PLI30_02405"
                     /inference="COORDINATES: protein motif:HMM:NF013456.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HOI78437.1"
                     /translation="MVKILVSLGSNIYSKQNIDRARRMLAHYFPDVVFTPSIITTATD
                     EKYSFPFRNVLAVFYSDLPSEEIVQKLKMIEFAMGRQPRDKDTGKVTIDIDLLQYGDE
                     ILRPDDYERAYVQALLTRLIS"
     gene            11195..>12123
                     /locus_tag="PLI30_02410"
     CDS             11195..>12123
                     /locus_tag="PLI30_02410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019539166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nodulation protein NfeD"
                     /protein_id="HOI78438.1"
                     /translation="MIYKINIKENIGSNTWVYLQNGMHQALNKNADCILLHMNTYGGS
                     VTEADSMRTAILNFQLPVYVFIDNNAASAGALISIACDSIFMRSAASIGAATVVSGQD
                     GSKAPDKYQSYMRGMMRATAESHGRDTVIQNRDTLIEWKRDPRVAEAMVDEKIVIPGF
                     ADSTQILTLTASQAVELRYCDGIAENINQIAVQYLGYRDYDLETYNPTLYDRIKGLLM
                     NGVLQAILIMLIIGGIYFELQTPGIGFPTAVAVTAALLYFTPLYLTGYAQNWEVLLFV
                     LGLILIVFEIFVIPGFGVAGISGIILVFTALILA"
BASE COUNT         3278 a         2598 c         2819 g         3428 t
ORIGIN      
        1 cagcatcacg cccgaaatta tcgacagcct gaaagaattt gtgggatacg ataagatccg
       61 gatcgacagt gaaggaaatg tcgtaaaatc cgatccgcgg ctacagttaa acacttccgc
      121 catatccaaa ggctatgcct gtgatttggt tgcccgtttg ttcgatagct atgggattga
      181 aaattacatg atagagattg gcggtgagat cgtgaccaaa ggcgtgaacg ataaaggtgt
      241 atgctggcgc atcggagtag ataagcctat cgatgacagt acgggtatgc agcatgaatt
      301 gcaaaccatc ttatcgctgt gcgataagtc gctggcgaca tcggggaatt accggaattt
      361 ctacgtgaaa gacggaaaaa agtatgccca taccatcgac ccgcaaaccg ggtatccttc
      421 ggaacaggat attttaggcg caaccgttat tgctgaagat tgtatgactg ccgatgccta
      481 tgccacggca tttatggctt cgggattgga aaagtcgaag gagattgccc ggaaaatacc
      541 gggactgcat tattatttta tttacgtgaa acccgacagt acgctggaca tagcttattc
      601 cgaagggttt gaacagtttt ttgccaactg atatgaacaa cattgtttcc attgttcctc
      661 tgaacaaaaa agaggtgaaa gatacggttg tcagctattt cttttatgaa accgactacg
      721 gactggcagt tattgcttcc acttcaaaag gtatttgtta tgtgggtttc ggagaaaagg
      781 aaccgatgct gaatgatttg aaaaagagat actccggcac ggctcttcag gaacaaaaag
      841 ccggtttaca taaactcgcg ttgagcttta ttaaaaacga aagagtttcg gagcttccat
      901 tacatattgc aggaaccgat tttcagttaa gtgtctggaa agcgttgctg caaatccccg
      961 ttggaaaatt atcttcctat aaagccatcg cgaaggctgt aaacaatccg aaagcggtac
     1021 gcgcagtcgg atccgctatc ggggataatc ccgtttccta cattattccc tgtcaccgtg
     1081 ttatccgctc cgacggcgga ttgggtggct acttctgggg actggagatc aaaaagaaaa
     1141 tgattgaaag ggaagcacaa acagcggaga tcgaaaatta agtaaatgtt aaacgtcaaa
     1201 caatccatgt cgttgaaaaa actattcgtt gctattttta tgatgttttc cctgctgaca
     1261 ccggcacagg aaacagattc cgttcgttat cccactttta aggttgatgg aacgatgaaa
     1321 aataaatttg agtatgcctc tgaaaccgga agttccaggt tctccgtcag aaattccagg
     1381 atgggcgtac gcggttacct gacccctttc ttttcctacc gtggacagat agagctcagc
     1441 gataacggta actttaaggt gctggatttg tacggttctt ttgaacccgt caggggactg
     1501 tctctttctc tggggcagac tagtattcca atttttaatt cttacgttgt ttcgccagcc
     1561 aacctgatgt ttgccaaccg tgcttttata ggaaaatatt ttataagcac acgcgatatc
     1621 ggttttctgg ccgattataa ttttaagatt gatgcggttc cggcttccat tgagtttggt
     1681 gcatataatg ggaacacgat caacgatccg gtctggcgtg aaaaattgtc gtacggcggg
     1741 cgtctcgaac tcggtagtat gaaagggctt cggtcgacgt tcaagtttta cgattacttg
     1801 aatacaccta cagtccatta ctttttttac ggagcagatt tgcgttatga gggcgctaac
     1861 tggaaagtag aaacggaatt tatgaaacgt gataacaaag aggtcgacga agatcagatg
     1921 ttttcctatt acctgcaggg cgcttatgca gttcctctta aggaaaccta ttttttcaag
     1981 aatattgtgc ctgccgttcg atgggatgcc attgacaagc atatgaacga aaagggtttt
     2041 gatgtggata ggttgacagt cgggcttggt tttgggctta ctaaaaaata tttttcatcg
     2101 atattgcggt tcgattacga atggtatttt ataaatcaag agcttgatat tttgaatctc
     2161 tacgaagaaa tggattcgga caagtttaca gttgaattat tattgacctt ctgaaaaatt
     2221 taaaactatg gcaaaaaaaa ttctatcgtt cttgtgtgtt gtttctctca tattgccggg
     2281 ctgtaccgag cagaacaaga aaaaaatcct ttttgacgga aaagatgtga cgaactggct
     2341 gactgaaggg ccggtgacgg tttcggattc cgtagtccga atggatggtg tcggtaaaat
     2401 gaccttgaaa aacggaatgt tcaccgattt tgaactgctt cttacggcaa aaacggtgga
     2461 taacgggaag ggagaggtcc gttttcatac cgatgaaaac ggtaacggcg gttacgctgt
     2521 tgcgctcaac aacgatctgg atcatcaaca atggtggaca aaaacgggaa gtttattgtc
     2581 agtcaggaac ctgacaaaat ctattgtgaa aaacaacgaa tggtttaacc ttcgtatccg
     2641 ggtagaaggt aaaaagatag aggtggctgt taacgatgaa ttactagtag actatattga
     2701 acctgcacaa ccgtaccgta caccggaaaa caggtcgcaa atcctttcag gaggaacttt
     2761 ttgcctgcaa agtactgaag gtatcgttga agtgaaattc attgaagtta cgccgttaaa
     2821 aatcgaaaaa acggtgattg acagccagct tgtccaagct atcgacgaat catcggacga
     2881 gatcataaaa ctgcatcagg cgaatttccc ggtactcgat tatcacgtgc acctgaaaga
     2941 agacctgacg ttggagctgg ctaggtccca atcacgcaag tatggcatca attatgcatt
     3001 ggcacccaac tgcggtatag gatttcccat tcaaaatgat gcgcaggtac tggagtattt
     3061 caatggaatg aaaggccagc cgtttgttca ggccatgcag ggagaagggc gtgaatggcc
     3121 ggccactttt tctaaagaag tgcgtgacct gttcgattat gtttttacgg atgccatgac
     3181 gtttacagat cgaaaaggca acagaacccg cttgtggatg ccggatgaag tattcataga
     3241 cgatgaacaa aaatacatgg atctgattgt tgagaacatt gtgaaggtta tggacgaacc
     3301 gatggatgtg tatgtaaacc ccaattttct tcccgatgcg atgaacgacc gttacgacct
     3361 tttttggacg gatgaacgtc aaaacaaagt gattgaggct atggtgagaa ctcataaagt
     3421 attggagatc aaccatcggt ataagatacc gaacaagtct tttattcaga aagccaaagc
     3481 tgccgggctg aaattcacat tcggtaccaa caactccaat tccgatttcg gtaaaatgga
     3541 atattgcata cagatgatga aggaatgtgg cattactgca caggatatgt ataaacccgc
     3601 tatgacagag taaaaaagct gtgggtaatt agtttttcat ccttttgtaa tcccttgcga
     3661 gcccgccctc tccggtttct ttgtagagcg agggtaaatc atggccggtt tgtttcatta
     3721 cttcaaccac tcggtcgaac gataccagat ggataccgtc ggtaaacgat gagtagaggt
     3781 tggcatccaa tgcgcgcgca gcggcataag catttcgctc gatgcaaggt acttgtacca
     3841 gtccgcaaac cggatcgcat gtcattccca ggtgatgctc caatcccatt tcggccgcat
     3901 attcaatctg agccggacta cccccgaaca actggctggc tgctgctgcc gccatggagc
     3961 aggctacacc cacttcgccc tgacaaccca cttcagcccc cgatatcgat gcgttttgct
     4021 tgaccacgtt gccgaacaaa ccggctgtag ccaacgcccg caagatgcgg gtttcgctga
     4081 aatcgcggct ttcttccagg tagtacagga tagctggaag tactccgcac gaaccacacg
     4141 taggcgcagt gacaatcttt cccccggagg cgttttcctc cgagaccgcc agcgcataag
     4201 caaacaccag cccgcgcgac tgaagcgatg ccttgtagcc tttggctttg atgaaatagg
     4261 atgcggcttt gcgttgcagg ttcaacggac ccggcaacac cccctcgtta tccaaaccac
     4321 tttttacggc tgctttcatc acctgccaca cgtcccgcaa atgctcccaa atatcattat
     4381 cctcatgtat ttcaacgtat tcccagtacg ttctgccgtt ttgctcacac catgcctgta
     4441 tatcggcaat ggttgtcatc ggatagatat ctttttgtgc cgcttccgat agccccacaa
     4501 cagtggtacc gtcgcttaat gctccgccac ctacgctgta gaccgtccaa gaatcgagtt
     4561 tattgcctgt tgcgtcgaga gcgtcgtacc tcatcgcatt gggatgaaac ggcaggaatg
     4621 tcctgggtag ccactcgatg gtggttggga catgcggttc caacacgttc aggatggcag
     4681 tgtcggttaa atgccctttt ccggtagctg ccaggcttcc gtacagcgtt accacgtatt
     4741 tcgcagctcc gggagccctt tccaggaata gctctgcagc ctttttaggt cccatggtgt
     4801 ggctgctgga cgggccgttc ccaattctat acagttcttt taatgatttc atgtttactt
     4861 ttaaactttc ttcagtacgt ctccctaata tgccttacgg taaccggtgt ttttgctgat
     4921 gctttcagtt cgggagaggt tatttctatt accttttttt ctcccggaag cagatcaatg
     4981 aagttatcgg taaacttcgc gcccattaca gggatctcga taaaaacgtc cttcgccaac
     5041 tttttgcttg tgagcgtaac ggtatatttg ccgtcggcat attttaccga agtttgtacg
     5101 gtggtttgcg gcaggtttag tttgttgggc cagtagaaga aatgatcttt gacggaaagt
     5161 ttttttccct tgctgtcggt taaccacgct cggatcatcc tgtttgactt atcggcttca
     5221 gatagtaatt cttctacgtt gaaagttttt accacgttgc tggagttggc ttttgcgttc
     5281 actttttcct tgaactgttt cactacgcct ttgttgaaat ccactacctg cacggttaac
     5341 tgcaagccat tcctatcgat gaggaagtcc gacagggtgt aataattcag ttgattatcc
     5401 ttgaattcta cacccaatgc caacggagcc agcacgtctc gcatgcggta gtgttgtgct
     5461 ttccagttgc cgtaataatc gatgctcgac caggaaacca ccggccagtc atcgtttatc
     5521 tgccaataga gtgcacccat gcaataggga cggccacggc gcatagcttc aacggattct
     5581 tccataccgt tgccttgcat taccagtccg atataaacga aatcttcaaa attccggggt
     5641 tcgtggtaat acatgtccat gtattttttg atgagtgagt ttccggtgga tgctttttga
     5701 tgaactttca ttacttcact ttccaggctc cattcatttt caggtgagaa agagcggatg
     5761 gttttcattt cggggaacga ctggaagccg aattcactgg caaagcgcgg aagtcggccg
     5821 gccattcctt cgaacggcag gtgtccgtac cagagccccc agtcgtgcac atcgctcgat
     5881 gcgaacgttt cgggatttcc ccagttggaa tcgtatggcg aaccgtggat atagctgcgt
     5941 gttccgtcga actcatttac caggccagga atgagttcac ggaacgtctt gtcgtaacct
     6001 tcgagccaga cgttgtaaat atccgccggg tattgtttgt cccaacccca gtgttgaagc
     6061 ccttcttcca cctcattgtt cccgcaccag aacgccaggg aagcacggtt tcgcaggcgt
     6121 ttgaggttat atataacttc gtctttcacg ttggccagaa aacgatcgtc ggacgggtag
     6181 ggcacacagc cgaaaatgaa atcctgccaa accaggatgc ctcttttatc ggcctggcga
     6241 taaaattctt cgttctcgta gattccgccg ccccacacac ggacaaagtt catatttgcc
     6301 gcttcaatgt tatcaaaaag tttggtgtaa taatcttcgt tttgttgagt agtgaaaatc
     6361 tcgcccggga tataattagc gcccttggca aagagaggaa tgccgtttac ttcgaaataa
     6421 aatgattttc cgtacttgtc cggctcctga accagtctga cagaacgtaa gcctattttt
     6481 tctgtttttt cagtaattac ccggttttcc gcagcaacgt ttgctgtgaa atcatacagg
     6541 tgttgatccc cccagccggt tggcatccat cttttgggat atgcaatttc caactccaag
     6601 aaaacggtat tgttgcctcg gtgtagcgtt acttgttttg ataccgatct tttggcttca
     6661 cccgctgcgg catattcaac cgtaacagtt gcagaaacag gtttttcggc gatggaaaac
     6721 acttcgacta tattttcgat tttagccgat tgtgctgtaa cagatgtctg ttttacgaaa
     6781 aaatcatcga tccgtacctt gtcataaaat accaaggata ccggtttcca gatccccatc
     6841 tgtgctatgc ggattcccca gtcccaacca aactggtaag gtgctttccg gttgtatatg
     6901 ctcagttttt catcgcggtg gtcgtttccg gccggataat cgtaccctgc ggtcagccgt
     6961 gcaggcatca gcgattgtac gggtgaataa aaccggatgt ataaactgtt ttcaccctct
     7021 ttaagtatgt tttttaccga tattttgtag ccaacaaaca tattttcgga acgtagaatc
     7081 cgagcgccgt tcaggaaaac atcggcttgc gtgtctaatc cctcgaagaa gataaccgca
     7141 tcgtcatgcc tgagttgttc ggcagaaacg gtgaacgttt ttttgaaatc ccagttttcg
     7201 tcttctaccc attgaaccag cctttcgttg gtcccgtaaa acggatcggg caacacttta
     7261 tgacggatga ggtcgtgctg taccgaaccc gggacctgag cttcgtacca ggtgtcctta
     7321 ccggtttgcg agaatgtcca cccttcgttc aaccataacg tttctccgtt tttctgtgcg
     7381 tacccaaaca ccggcaaaaa tgccaacaac caaagtgata gttttttcat ttcttttatc
     7441 aatccttttt acagggtttt taatcggata tttctgaact ccactttcat gggggggcct
     7501 acatgaacct gaacgcctat taatccttgt ttctttccgt taagcaaatc attatccgtt
     7561 acctcactca ttaaaacacc gttcagaatg tgcttcattt tattgccgtt tacaattagg
     7621 tgacaggagt tccaatcgtt atttttgatg tggcttttta attcggcaac atcggctaat
     7681 tcaactattt ctgcttttgt ccaggcattg ttcttgacca ggtccgaaac cggaaggctg
     7741 gataacgagt caggtaaagg atttaacacc accgtttgtc cttgataggc gagtgttgtt
     7801 ctccgtcgct cttcgtagtt ctgccctgta tagttgtttt tgccgtcgat atcaaactgg
     7861 tatcccctga gtgcatgggg aagatctgca atttcctcac tcctgtagtt gataccgctg
     7921 ttcccgtctt cgctaatgcg gtattccaat tttaattcaa agttttcggg agctccgtct
     7981 tgccagacaa tgaaagagtt tctcgataaa acggtttcag gagttatctc accggtaaga
     8041 ataccgttct ctatacgcca gtaagtggag tcgcctttcc atccgtccaa cgatttccca
     8101 tcgaaaatag gtacaaatcc atcgtcggtt tttttgttct gacatcctgc gacaattccg
     8161 aaaattaaca ggactgcggg aatgaataag tttttcattt gcttgttttt agaaatacaa
     8221 aagtaataaa taaaccgcta attggaaaag attgcagtat ggaaacctga taacaaacct
     8281 gattttttaa tctttcccgt gacaagttta tgggtaaaac agccaattta tttcaaaaaa
     8341 ttgtaatttt acgttacaaa ccggatatga gctgacaaaa atgaaaacaa tacccactat
     8401 atctttcgat gactatgttc ttttgcaccg gaggctgtat ggtttattcc ggcagagatg
     8461 gagcttgcag aaaaccggct ttttgaaaag agtctgggag gattctctgc agaggtccgt
     8521 agatgaaggc tcggaagatt tgttgcaagg ccttttgttt gatatgaaaa cagcggtgat
     8581 attagtcgac gagattggct taaagtatgc agtggtctgt gcggttttaa tgttcaggcc
     8641 gttgatgaaa ggctatgtta cggttgatga aatcaggaag gagtttggtg aagaggtagc
     8701 tgtcattatt cggggtttga agaaagtgaa cgacctgact gaaaaaaaat cgcgtgttga
     8761 gtcggagaat tatatcaagc tgctcctctc catagccgaa gatatccgtg tggttttcat
     8821 actgttggcc gaacaattgc agttgatgcg cgatgcgaaa aagtcgactc cggaaaatcg
     8881 attggatctt tccgttgaat ccacttacct gtatgcgccg ttggcacacc gattgggttt
     8941 atatactatt aagtcggaac tggaagactt gtcgctgaag tatacggata gggagactta
     9001 cgatttcatt gcccggaaac ttaatgaaag caaacgttct cgagataaat atatcgagga
     9061 gtttattcat ccgttaaagg aacgtctgga taaaacaaac ctgaaatacg atatcaaagg
     9121 ccgtacaaaa tccattcatt ccattaataa caagctgaaa aaacaaaaga ttgaattcga
     9181 taatatttac gatctctttg ctatacgcat catccttgat tctccttcgg aaatggaaaa
     9241 ggcacattgc tggcaagtat attccattgt gaccgatatg tatcagccaa atccgaagcg
     9301 gttgaaagac tggttgtcca tacccaaaag caacggatac gagtcattac atatcacggt
     9361 gatggggccg gactcaaaat gggtggaagt ccagatcagg acccggcgaa tggatgatat
     9421 tgccgagcgg ggtttggccg cccactggaa atataaggga ataaaagaag aatccacgct
     9481 ggacgattgg ttgaaatcgt tgcgggaatc cctggagagc agagatgtgg atatcaggga
     9541 taaactgggc gattttaaac tcgatttgta cgacgaggag atttttgttt tcaccccgaa
     9601 aggtgatctg ttcaaacttc ctaaaggtgc gtcggtcctc gattttgctt ttgccataca
     9661 ttctaaattg ggtgctactt gtgtgtctgg aaaggtgaac ggaaagaatg tgccgataaa
     9721 acacatgctg aaaagcggag accaggtcga gataaacacc tcgtcgcacc agtctcccaa
     9781 acaggactgg ttgaattatg tggtgacctc gaaggccaaa acccggattc ggcagttgct
     9841 caaggaagaa gcaggaaaac aggtggatat tgccaaggaa atgttgtcac gacggatgaa
     9901 aaaccgcaag atagacctgg acgatccgct gttgatgcaa ctgattaaaa aattaaaata
     9961 taaaacggta acagactttt acatcgatat cgccgctgag aaactggaag tgaactgggt
    10021 gatagaccgt tacttggaat tgggaagtaa agagtttgaa cacagggact tcctttcagc
    10081 tactagcgct gatagttttg tccttaaacc atcttcgcaa gaaataaaaa gctctgacga
    10141 gcttattatt gatcagaacc tgaccggggt ggactaccgg ttggcaaaat gctgtaatcc
    10201 catttacggt gatgagattt ttggatttgt gtcgtcgcaa ggaattaaaa ttcaccgtac
    10261 aagttgtccc aacgcacacg atctgttctc gcgttttggt tatcggattt taaaagcccg
    10321 gtggtcgggc aaaacctcgg ggacggcaaa ctatacatcc gtactgcgca tcattggaaa
    10381 tgatcagctc aatattgtag ctaacttgat gtcgatcatt tcaaaagaag acggagtgca
    10441 gatgcgctcc atctccatcg attcaaacga cggattattt cagggaaaca tcgctgtaat
    10501 gctggctagc acctctatgc tggaacaatt aataaaaaaa ctgaaagcgg tgaaaggggt
    10561 aaaatctgtt tcgagattga attgaactga gctgctagta acggggcatt ggaggagaag
    10621 ggtttatttg aaacatgctg aatcaactga ttaatcgggt caataatgct tgcacataag
    10681 ctctctcata gtcatccgga cgaagaattt cgtcaccata ctgaagcaaa tcgatatcta
    10741 tagtaacctt tcctgtatct ttatcgcgcg gttgccgtcc catagcaaac tctatcattt
    10801 ttaatttttg aacaatttct tccgatggta aatcgctgta aaaaactgcc aatacattcc
    10861 ggaacgggaa agaatatttc tcatcagtcg ccgtagttat tatcgatggg gtgaaaacaa
    10921 catcggggaa ataatgagcg agcatccttc ttgccctgtc aatattttgt tttgagtata
    10981 tattcgagcc taaactgact aagattttaa ccatagtaga tttaaatttt aaaaggcaaa
    11041 tataatcagt tttttttaaa aggggcaacc tagaaacgaa aaacaactat ctttgtagaa
    11101 ataaacctgt tgaatgatga aaaatatcag gctgattttc gttttgtttt tcattctgtt
    11161 tgtctatgtg acgtatggac agcattcaaa tccaatgatt tacaaaatta atataaaaga
    11221 gaacattgga agcaacacct gggtatattt gcagaacgga atgcatcagg ccctgaacaa
    11281 gaatgccgat tgtatacttc ttcacatgaa tacgtacggt ggctctgtta cagaagccga
    11341 ctccatgcgt accgccattt taaattttca gcttcccgtt tacgtattta tagataacaa
    11401 cgctgcttct gccggtgcac tgatttccat tgcgtgcgac agtattttta tgagatcggc
    11461 ggcctccatt ggagcggcaa ctgtagtgag cggccaggac ggttccaaag ctcccgacaa
    11521 ataccagtcg tacatgcgtg gaatgatgcg cgcaacagca gaaagtcacg gaagagacac
    11581 ggtcattcaa aacagggata cattgataga gtggaaacgc gatccgagag ttgccgaggc
    11641 aatggtggat gaaaaaattg tgatacccgg ctttgcggat tctacccaga ttctgacgtt
    11701 aacggcaagt caggccgttg agttgcgtta ctgtgacggc attgcggaaa atatcaacca
    11761 gattgccgta cagtacctgg gttatcgtga ttacgacctt gaaacgtata atccaacgct
    11821 ttacgacaga attaaaggct tattgatgaa cggagttttg caggccattt taatcatgtt
    11881 aatcattgga ggaatttatt tcgaattgca aacgccgggg atcggctttc ctaccgcggt
    11941 agctgtgacg gctgccttgt tgtattttac gccgctttac ctgaccggct atgcgcaaaa
    12001 ctgggaggtt ttgttgtttg tattgggttt aattttgatt gtctttgaga tttttgttat
    12061 tcccggattc ggagtggcag gtatctcggg gataatactt gtttttacgg ctttaatact
    12121 tgc
//
LOCUS       DAOSDR010000034        11778 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrimonas sp. isolate AMR_MDS_1826
            NODE_2102_length_11778_cov_4.382581, whole genome shotgun sequence.
ACCESSION   DAOSDR010000034 DAOSDR010000000
VERSION     DAOSDR010000034.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863355
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrimonas sp. (wastewater metagenome)
  ORGANISM  Petrimonas sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Dysgonomonadaceae; Petrimonas.
REFERENCE   1  (bases 1 to 11778)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11778)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:10:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,720
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 35
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11778
                     /organism="Petrimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2102_length_11778_cov_4.382581"
                     /isolate="AMR_MDS_1826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2023866"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..832)
                     /locus_tag="PLI30_02415"
     CDS             complement(<1..832)
                     /locus_tag="PLI30_02415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005866190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HOI78439.1"
                     /translation="MTYKWNYLTLTTDQKNKKNELTKEIQIDPVLTELLLKRGISSVE
                     EAQKFLYPSLSDLHDPFLLPDMEEAIRRIEQAIGNKERILIYGDYDVDGTTAVSLVYK
                     FLRKITNNIDYYIPDRYDEGYGISIQGIDYAVETDVKLIISLDCGIKAIKKVAYAKEH
                     GIDFIICDHHMPDEELPDAVAVVDAKRADSIYPYNELSGCGVGFKLIHAFSIRNGLAF
                     SDIEPLLDLVAISIAADIVPITGENRVMMHFGLKRLNANPSFGLRGIIEICGLSKKPI
                     T"
     gene            complement(1030..2568)
                     /locus_tag="PLI30_02420"
     CDS             complement(1030..2568)
                     /locus_tag="PLI30_02420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007666698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HOI78440.1"
                     /translation="MSAAHSSHPKGLYFIFITGMSERFSYYGMRAIFTLYLINALMLD
                     KEFASAIYGNYTGLVYLTPLIGGYVADRYLGMRRSIFWGALLMVMGQFLMFFSALHFE
                     NTELAKWLMYGGLGGLIFGNGFFKPNLSSIVGRLYEPGDKRLDSAYTIFYMGVNMGAF
                     MAPLLCGYLGDTGNPADFRWGFLAAAITMVLSLVFYVARNSRYLVGPNGEAIGIVPAQ
                     KAGRESAGELGVDLGKKLNVWQLSLWAVGGVLLFFLFLKGFDGDIIGAVIYSACIVVP
                     GFVISDPTLTKIERQRILVIYIIAFFVIFFWAAFEQAGISLTYFAEEQTNRHVFGWTM
                     PASWFQSFNAVFVVLLAPLFSALWIKLGQKNREPASPTKQAIGLFLLSLGYLLIAIGV
                     KNVAPDAKVSMLWLTGLYFIHTMGELTLSPIGLSMVNKLSPVRFASLLMGVWYLSMAT
                     ANKFAGTLSSLYPEVGKTKIFLGFEVTTLFDFFMIFVVMSVLASIVLFVLSKTLQKLM
                     HGIR"
     gene            2671..3735
                     /gene="mnmA"
                     /locus_tag="PLI30_02425"
     CDS             2671..3735
                     /gene="mnmA"
                     /locus_tag="PLI30_02425"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005650427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004808 - tRNA
                     (5-methylaminomethyl-2-thiouridylate)-methyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0002143 - tRNA wobble position uridine
                     thiolation [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HOI78441.1"
                     /translation="MEIAALVSGGVDSSVVVHQLKEMGYDPTIFYIKIGMEDEQGYID
                     CPSEEDIEIVTFIAKKYGCRFEIVSLHEEYWNSVVQYTIDSVKRGLTPNPDMMCNKLI
                     KFGTFEQKWGHHFDKIATGHYATTTESEGLTWLSTAKDHVKDQTYFLGQVSYVQVAKL
                     MFPIGNLLKSEVRKIAADNKLPSAQRKDSQGICFLGKVNYNHFIERYLGKKEGLIVEL
                     ETGNIIGRHQGFWFHTIGQRKGLGLSGGPWFVIKKDVNRNIVYVSKGYNTRHQYGNMV
                     NLQGFSFITKDIWGDFDGEKEVTFKIRHTPEFTRGRISKTGDLYSISSGLPIQGIAAG
                     QFGVVYDTESHLCLGSGMII"
     gene            3735..4370
                     /locus_tag="PLI30_02430"
     CDS             3735..4370
                     /locus_tag="PLI30_02430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008619139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimeric intracellular cation channel family
                     protein"
                     /protein_id="HOI78442.1"
                     /translation="MELIENIQFVNIIEFLGTFAFAISGVRMASTKNFDLFGAFTIGF
                     VTAIGGGTLRDLFIGVTPFWMLNPVYLWATLLALLFVLLFKNKVVRLQYTFFLFDSIG
                     LGLFVVVGAEKTLAMGFAPWVAVIMGTITGSFGGMLRDILITQIPLIFRKEIYALACV
                     AGAIFFTMGYALDLNIVVNEVATASIVILTRILAVKFQWHLPILKGEKNKL"
     gene            4371..4883
                     /locus_tag="PLI30_02435"
     CDS             4371..4883
                     /locus_tag="PLI30_02435"
                     /inference="COORDINATES: protein motif:HMM:NF024895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HOI78443.1"
                     /translation="MGYDMKRLIGLSILLLALFPSKSFAQYEGTVGLGVHAGYSNKAK
                     NVGAGIHLHYYHTNQLRFAPSFSCYLPSKGNSMWEADADAHYIVPLSWLFSFYPVAGL
                     NYSNRKFDASKTNDTGLQDWTKHRIGANLGLGIQYDFGYKTRISVEYKYRFIKDFSQS
                     SFTAGIGFWI"
     gene            4921..6153
                     /gene="hflX"
                     /locus_tag="PLI30_02440"
     CDS             4921..6153
                     /gene="hflX"
                     /locus_tag="PLI30_02440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009317979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HOI78444.1"
                     /translation="MKDFITSEIKNENAVLVGLITPEQSEEKVNEYLDELAFLAETAG
                     LVPGKRYVQRLDMPNAVTFVGTGKLQEISEYVLDEDNQVGVVIFDDELSAKQIRNIEK
                     ELKIRILDRTSLILDIFAMRAQTSHAKAQVELAQYQYLLPRLTRMWTHLERQRGGGGV
                     IMRGPGETQLETDRRIILDRIARLKNELKDIDKQKTVQRKNRGKLVRAALVGYTNVGK
                     STLMNLLSKSEVFAENKLFATLDTTVRKVTIENLPLLLTDTVGFIRKLPTNLIESFKS
                     TLDEVREADILVHVVDISHPAFEEQIDVVEKTLYDIDKTKKPTILVFNKIDAFSHVEK
                     EEDDLTPKTRQNTSLEELKQSWMSKLNENAIFISAKEKTNIEELKSLIYERAREIHIQ
                     RFPYNDFLYQTYDEETEI"
     gene            6229..6543
                     /locus_tag="PLI30_02445"
                     /pseudo
     CDS             6229..6543
                     /locus_tag="PLI30_02445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013612047.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="beta-eliminating lyase-related protein"
     gene            complement(6535..7917)
                     /gene="creD"
                     /locus_tag="PLI30_02450"
     CDS             complement(6535..7917)
                     /gene="creD"
                     /locus_tag="PLI30_02450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019538430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein CreD"
                     /protein_id="HOI78445.1"
                     /translation="METEKRNLIERYSTTIKAFSVAILTVMLLIPLAMIQSLIRERQN
                     TRQGAVQEITSKWGGEQTVTGPCLVVPYEEIRVEDKKEITVNRNLLVLPETLDVKIST
                     RVEKRKRGIYDASVYGSDIVLSGQFDGSVITRQNVQLSQVKWDDVRIIIGLSDLKGIK
                     DNVSLQSNGQTTTFDPGIPVENLETGRGEAGKNANIAYELFRVGLNAKMDSLNPDSAL
                     QETFPFTVSLKLNGSHGLYIVPTGKTTTAAIRSDWATPSFDGEFLPQTRDIDKAGFSA
                     EWRVLDLNRSFGQAIRSDDSNAINQMASSRFGVRFIQAVDQYQQNMRSVKYGILIVLL
                     TFVVVFFIELLQKKPVNPFQYILVGLALVLFYTLLLSMSELLGFNLAYLAAAVMTTLL
                     IMFHMGSILKSRKNGFLVGTLLAFLYLFIFILIQMESYALLVGSLGLFAILAVIMYYS
                     KKVTSSLFSF"
     gene            complement(7946..8248)
                     /locus_tag="PLI30_02455"
     CDS             complement(7946..8248)
                     /locus_tag="PLI30_02455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013612986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HOI78446.1"
                     /translation="MKEFLEDINKAFESKVRLGIMSMLMVNNEVDFNTFKEMLGLTDG
                     NLASHLKTLEGLGYIEVKKEFIERKPRTTYSRTCEGEQAFKEHLQALEAFIKQATD"
     gene            complement(8245..8877)
                     /locus_tag="PLI30_02460"
     CDS             complement(8245..8877)
                     /locus_tag="PLI30_02460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008765007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI78447.1"
                     /translation="MKNIEAIETVKEIRAMMEKSSRFISFSGTSAILIGLYALGGSWI
                     ARSVLTSVNSVPDGYSAPLWLAVAPQLIITALLVFVLALGTILFFSVRKAKKRQQSFF
                     NKPAYRTFFNFFLPLTTGGIFCLALLLNGYVGIVAPVMLLFYGVSLINAAKYTYGSIF
                     GLGCAEIVLGLLCAFFPGKGLLFWSAGFGVLHILYGIYFYVSIERKERLS"
     gene            complement(8955..>11778)
                     /locus_tag="PLI30_02465"
     CDS             complement(8955..>11778)
                     /locus_tag="PLI30_02465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019540510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HOI78448.1"
                     /translation="MRHLVYSRTDLAGGVASEITLAKPGNIEEEWRDYRDLFVIAFPT
                     PEGDTGARLIPSRITSNRFGLSWRDCLVDRKTLTIPPSVDEPTVLNITFPEKITLRTV
                     EFPPIDSYSHARVYAPGVQVAVYANLPGERRRVAFIDMPAANWQDDQPLSIACAETPS
                     DSYRIEISNLHEMTLPYIHFYSAARQQNWESEAAWTLRRIVREEYPQQDPRSWVDPST
                     IVDMSNHMDSSGKLNWTVPAGNWTVLRIGHVNTGQKNGPAPPEATGWEATKLHPSGIR
                     ANFDGYVGRLIKGDGALKDGLLQGVLLDSWECKTQTWTADLDKIFDNQWSYALRSRLP
                     ALFGYVVDNPENTARFLRDWRVTLNDLLVENFFGEIKKLADENGLTVSFETASGDVFP
                     GDILEYYKHADVPMCEFWQPRSDSFVGSIEFKPVRPAVSAARGYGKKRVAAEAFTSFN
                     LTWDEHPRFLKDIADDHFAKGVTHLVFHTYTHNPRTDFLPPGTSFGTKIGTPFLRLQT
                     WWQHMPLFTDYLARCNYMLETGNPVSDVLMYLGDEQNHKPPQLLPFPEGYSYDYCNPD
                     ILLNRLSVKNGKLVTPEGIQYRVLWLYDCRRMLPETLEKIASFVEAGVILAGDAPSGI
                     ATLSGGDETKLRFDKAVGKLWGDGSKNMLTLGKGKVYNTSDIATVLTAENIPPDILAH
                     SPDLRWLHRQTGESGIYFLSTSPEKGYRGLASFRIRGNAELWDPLSGEIVAVNSNVKD
                     NYTEVELDIPAGSSCFLVFKNRKSNKPLRQPQPTEKLDLSQASWQVYFPGGWGVENSP
                     VVTQKLIAWKDFPVSEEGKSFSGTATYETTFTLDRKSGTTAYLLNLGRVEVIARIRIN
                     GQEVGTCWTSPYVLDVTRYLQEGENNLQVEVTSTWFNRLVYDAGLPEESRRTWTIAGP
                     EAKAPLKEYGLMGPVSIELVSRYKNN"
BASE COUNT         3245 a         2912 c         2714 g         2907 t
ORIGIN      
        1 ctgtaatggg ctttttactt aatccgcaaa tctcaataat tccccgtaat ccaaaactgg
       61 gattggcatt caaccgtttc aacccaaaat gcatcatcac ccggttctct cccgtaatgg
      121 gcacgatatc ggcagcaata cttatcgcca ccaaatcgag caacggctcg atatctgaaa
      181 atgccaagcc gttacgtatg gaaaaagcgt gaatcagctt gaatccgact ccgcagccgg
      241 aaagctcgtt gtaaggatag atggagtctg cccgtttggc gtctacgact gccacagcat
      301 ctggcagttc ctcatcgggc atgtggtggt cacaaataat aaaatcgatt ccgtgctctt
      361 tagcgtaggc tactttttta atcgctttta tgccgcaatc gagggagata ataagtttaa
      421 cgtctgtctc aacggcataa tcgattccct gaatcgaaat gccataccct tcgtcgtacc
      481 tgtccggtat ataataatca atgttattgg ttatcttccg taaaaactta taaaccaacg
      541 aaacggcggt agtgccgtct acatcataat ctccgtatat aagaattcgc tccttgttcc
      601 cgattgcctg ttctatacgt cttattgcct cctccatatc gggcagcaag aatgggtcgt
      661 gcaaatcgct gaggctcgga tacaaaaatt tttgggcttc ttccacgctt gaaatgcctc
      721 gtttcagcaa caattctgtc agtacgggat caatttgtat ttctttcgtc agttcatttt
      781 ttttattttt ttggtcggtt gtcagggtaa gataattcca tttgtaggtc atttatatag
      841 tttttttctt tctctcggaa accggacatt gtccggtgaa aggcaaccat ccattcacac
      901 ttgaagcttt gttgccgtgc gaaattgtat ctatttatag ttatcgcaaa atattaattt
      961 gtaaagttag aaaaaaactt ttgaaatatc gatataattt gaccggaata aaaaaacagc
     1021 tttttctcct taccggattc cgtgcatcag tttttgcagt gttttggata agacaaacaa
     1081 cactatcgac gccaagaccg acataaccac aaaaatcatg aaaaaatcga acaacgtagt
     1141 aacctcaaaa ccaagaaata ttttagtctt tcctacttcg ggatagagag aacttaacgt
     1201 tccggcgaat ttgttggctg tagccatcga caaataccat acgcccatca gcaacgaggc
     1261 aaagcgtaca ggggacaatt tattaaccat cgacaacccg atgggagaga gggttagctc
     1321 gcccatggta tggataaaat acaaaccggt aagccagagc atgctcactt tcgcatccgg
     1381 tgccacattt ttcacaccga tagcaatgag caagtacccc agcgaaagta aaaacaaccc
     1441 gatagcctgc ttggtcgggg aagccggttc cctgtttttt tgacccaact taatccagag
     1501 cgcggaaaaa agcggagcca gcagcacgac aaataccgcg ttgaacgatt gaaaccagct
     1561 cgcaggcatt gtccatccga aaacatggcg gttggtttgt tcttcggcga agtaggttaa
     1621 agagattccc gcttgttcaa aagcggccca aaagaaaata acgaaaaatg caatgatgta
     1681 aattaccagg atccgttgcc gttcaatttt tgtcagtgtg ggatcagaaa tgacaaagcc
     1741 gggaacaacg atacaagccg aatagataac ggcaccgatg atgtctccgt cgaacccttt
     1801 caggaaaagg aaaaacaaaa ggacaccgcc aactgcccac agggataact gccaaacatt
     1861 cagtttttta cccagatcaa cacccaactc tcctgcactt tcacgcccgg ccttttgggc
     1921 aggaactatt ccgatggcct caccgttggg gcctaccagg taacggctgt ttctggcaac
     1981 atagaacacc aggcttaaca ccatagttat tgcagctgcg aggaaacccc acctgaagtc
     2041 ggccggattc ccggtgtcac ccaggtatcc gcacagcaga ggagccataa aagcacccat
     2101 attcactccc atatagaaaa tggtgtaggc tgaatcgagc cttttgtctc cgggttcata
     2161 gagcctgccg acaattgacg ataagttggg cttaaaaaaa ccattgccga aaataaggcc
     2221 gcccaatccg ccgtacatca gccatttggc cagttcggtg ttttcgaaat ggagagcact
     2281 gaaaaacatc aggaactgcc ccatcaccat cagaagcgcc ccccagaata tggagcggcg
     2341 catgcccaga tacctgtcag caacgtagcc tccaataagc ggggtcaggt aaaccagccc
     2401 ggtatagttt ccgtaaatgg cggacgcaaa ctctttatcg agcatcagcg cattgataag
     2461 gtacagggtg aaaatagcac gcattccgta gtaactgaag cgttccgaca ttccggtaat
     2521 aaaaataaag tacagccctt tggggtgaga ggagtgtgca gcagacataa cgttcttttc
     2581 tttgagacct caaaattata acttttagcc gaaacgtaac gcccaactac tatctatttc
     2641 gtaactttgc atccgaaaaa gaacaaaata atggaaattg cagcattggt gtcgggaggc
     2701 gtggatagtt ctgttgtggt tcatcagttg aaagagatgg gctacgatcc caccattttc
     2761 tatatcaaga tcggcatgga ggatgaacag ggctatatcg attgcccctc ggaagaggat
     2821 atcgagatcg tgacctttat cgccaaaaag tacggctgtc gcttcgaaat tgtttcgttg
     2881 cacgaagagt actggaacag cgtggttcaa tacaccatcg attcggtgaa gcggggattg
     2941 actcccaatc cggatatgat gtgcaacaaa ctgatcaagt tcggaacctt cgaacaaaaa
     3001 tggggacatc atttcgataa gattgccacg ggacattacg ctacaaccac cgaatcagaa
     3061 ggcctgacgt ggctttccac cgctaaagat cacgtaaaag accagaccta ttttttaggg
     3121 caggtgagtt acgtgcaagt tgcaaaactg atgtttccta tcgggaacct gttaaaatcg
     3181 gaagtcagga aaattgctgc ggacaataaa ctcccctcgg ctcaacgaaa agacagccag
     3241 gggatctgct tcctcggcaa agtcaattac aaccacttta tcgaacgcta cctcggcaaa
     3301 aaggagggcc tgatcgtaga gttggaaacg ggaaacatta tcggcaggca tcagggattc
     3361 tggtttcaca ccatcggcca gcgaaaaggc ctcggcttaa gcggcggacc ctggtttgta
     3421 ataaaaaaag acgtgaaccg caacatcgtt tacgtatcaa aaggatacaa caccaggcat
     3481 cagtatggca atatggttaa ccttcaggga ttttcgttta taaccaaaga tatctggggc
     3541 gacttcgacg gtgaaaaaga agttacgttt aagatccgtc atactccgga atttacccgg
     3601 gggcgcattt ctaaaaccgg cgatctgtac agcatttcct ccggcttgcc tatccagggt
     3661 atcgccgcag gccagttcgg agtggtgtat gacacggaaa gtcacttgtg cctgggaagc
     3721 gggatgataa tttaatggaa ttgatagaaa acatccagtt tgtaaacatt atcgaatttc
     3781 ttgggacctt cgcctttgcc atcagcgggg tgcggatggc atcgaccaag aatttcgatc
     3841 tgttcggcgc attcaccatc gggtttgtca cagctatcgg aggcggaacg ctccgcgatt
     3901 tattcattgg cgtaacccct ttctggatgc tgaacccggt ctatctttgg gcaacgctgc
     3961 tggcccttct gtttgtcctg ttgttcaaaa acaaggtggt caggcttcaa tacactttct
     4021 ttcttttcga cagtattggc ttggggctgt ttgtggtagt gggggccgag aaaacgctgg
     4081 caatgggttt tgctccgtgg gtggcagtta ttatgggcac gattaccggt tctttcggcg
     4141 ggatgcttcg cgacatcctg atcacccaga tcccgcttat cttcagaaaa gagatttatg
     4201 ccctggcatg tgttgcgggg gccattttct tcacaatggg atacgcactc gatctgaaca
     4261 tagtggtcaa cgaggtggca acggcatcca ttgtaatact cacccggata ttggctgtaa
     4321 aattccaatg gcatctgccg atcttaaaag gagaaaaaaa caaattataa atggggtacg
     4381 acatgaaaag actcatcggt ttatccatcc tgcttttggc cctgttcccg tcgaaatcat
     4441 tcgcacagta cgaagggact gtggggctgg gagtccacgc cggctatagc aacaaggcaa
     4501 aaaatgttgg agcaggcata cacctgcatt attatcacac caatcaactg cggtttgctc
     4561 cttctttcag ctgttattta ccgtcaaaag ggaacagtat gtgggaagcc gatgccgacg
     4621 cgcactacat agttcccttg tcgtggttgt tttcttttta tccggtggca ggattgaatt
     4681 attcaaaccg gaagttcgac gcatcaaaaa caaacgacac ggggttgcag gactggacga
     4741 aacaccgtat tggcgctaac ctgggactgg gcattcagta tgacttcgga tacaagacga
     4801 ggatcagtgt cgaatacaaa taccggttta tcaaagactt ttcgcaatcg tctttcacgg
     4861 ccggaatcgg gttttggata taactggcta gcctaatggc ctaaaaaatc tgcgagaaaa
     4921 atgaaagatt ttataacaag tgagataaaa aacgaaaacg ctgttctagt agggcttatc
     4981 acgcccgaac aaagcgagga gaaagtaaac gagtacctgg acgagctggc tttcctggcc
     5041 gaaacagccg ggcttgttcc cggcaaacgt tacgttcagc gattggatat gcctaacgcg
     5101 gtcactttcg ttggtacggg gaaactgcag gaaatatcgg agtatgtgtt ggatgaagac
     5161 aaccaagtgg gcgtggttat tttcgacgat gaactatcgg caaaacagat ccggaacatt
     5221 gaaaaagaac tgaaaatcag gatactcgac cggacaagcc ttatcctcga tatctttgcc
     5281 atgcgtgcac agacgtcgca tgcgaaagca caggtagaac tggcacaata ccaatacttg
     5341 cttccccgtt taacaagaat gtggacccat ctggagcgtc agcgaggagg aggaggtgtc
     5401 attatgcgcg gtcccggaga aactcagctg gaaactgacc ggcgaattat tctcgatcgc
     5461 attgcacgcc ttaaaaacga actcaaagac atagataaac agaagaccgt ccagcgaaaa
     5521 aacaggggaa aactcgtgcg cgcagctctt gttggctata ccaatgtagg caaatccacg
     5581 ctgatgaacc tgctgagcaa atcggaagta tttgccgaaa acaaactgtt tgccacgctc
     5641 gacactactg tacggaaagt caccatcgaa aaccttcctc tcctgttgac ggatacggta
     5701 ggatttatcc gtaaattgcc cacaaacctc attgagtcat ttaaatctac cctcgatgaa
     5761 gtgcgcgaag ccgatattct cgtccacgtc gtggatattt cacaccctgc gtttgaagaa
     5821 cagatcgacg tggtagaaaa aacactttac gatattgaca aaaccaagaa gccaacgatt
     5881 ttggttttca acaaaataga cgctttctcg cacgttgaaa aagaggaaga tgacctaacg
     5941 cccaaaaccc ggcaaaacac atcgctcgaa gaactgaaac agtcgtggat gagcaaactc
     6001 aacgagaacg ccatttttat ttcagcaaaa gaaaaaacca acatcgaaga gcttaagtca
     6061 ctcatctacg aaagagccag ggaaattcac atacaacgat tcccatacaa cgatttcttg
     6121 taccagactt atgatgaaga aacggagatt taaactgcaa tatatccata tcaaacgccg
     6181 ggacggctac ccttacttca atgcataatt gaaccttatc cctgctataa tgtaccgagg
     6241 gaagaattta tcgcccagac actgggtatc gagctgtata tggaagcggg aatccgaggc
     6301 gtggaagtag gagctttact agccgaccgc gatccgacaa cccgggaaaa ccgttacccg
     6361 gccctggaac ttctccggtt ggccataccc cgccggacgt ataccaacaa ccatatggat
     6421 gtagtagcag tagcactcaa aaacgtgtac gaccggcggg acaaaattac aaaaggttat
     6481 tccattacgt acgaagaacc catcatgcgc cattttacag ttgagctgga gcggtcagaa
     6541 tgaaaacagc gaagaggtta ctttcttcga ataatacatg atcacggcca ggatggcaaa
     6601 caatcccagg ctgccgacga gcagggcata gctctccatt tgtataagaa tgaatataaa
     6661 gaggtaaagg aaagcaagca atgtgcctac caaaaaaccg ttcttacggc ttttcaggat
     6721 gcttcccatg tggaacatga tcaaaagggt ggtcatgacc gccgccgcaa ggtacgcgag
     6781 attaaaccca agcaattcgg acatggagag caacagggta taaaacagca ctaaagccaa
     6841 cccgacaagt atatattgga acgggtttac cggcttcttt tgcaacagct ctataaagaa
     6901 aaccaccaca aaggtgagca agacaatgag gatgccgtat ttgacactgc gcatgttttg
     6961 ctggtattgg tcgaccgcct gaataaaccg caccccgaat cgtgacgatg ccatttgatt
     7021 aatggcattg ctgtcgtcgg aacgtatagc ctgtccgaaa ctacggttaa gatcaagcac
     7081 cctccattct gccgaaaagc ccgccttatc tatatcccga gtttggggaa ggaattcacc
     7141 gtcgaaactg ggtgttgccc aatccgaccg tatggcggca gtggtagttt ttcccgtggg
     7201 aacgatgtat agcccatgcg aaccgttcaa ttttaacgaa acggtaaaag ggaacgtttc
     7261 ctgtaaggcg gaatcgggat tgagggaatc cattttcgcg ttcaacccca cacggaacaa
     7321 ctcgtatgcg atgttggcat ttttacccgc ttctcccctg ccggtttcca ggttttcaac
     7381 aggaattccg gggtcgaagg ttgtggtctg cccattgctc tgtaacgaaa cattgtcttt
     7441 tatgcctttc aggtcggaaa gtccgataat aatccgcaca tcgtcccatt taacctgtga
     7501 aagttgtaca ttctgccggg tgattaccga cccatcaaac tgtcccgaca aaacaatatc
     7561 ggagccataa accgatgcgt cgtaaatgcc tcttttcctt ttttctaccc gtgtggagat
     7621 ttttacatcc aatgtttcgg gcaacaccag caagtttcta tttaccgtga tttccttttt
     7681 gtcctcaacc ctgatttcct catagggaac cacaaggcag ggcccggtaa cagtctgttc
     7741 ccctccccat ttcgatgtaa tctcctgcac tgctccctgc cgggtgttct gccgctcgcg
     7801 gatcagcgac tggatcatgg ccagcggaat aagcaacatc actgtaagta ttgccacgga
     7861 aaaggccttg atggttgtag aataccgctc gataaggttt cgtttttctg tttccatatt
     7921 tttttacttg ttagataaag cttttttaat cggttgcctg tttaataaat gcttccaatg
     7981 cttgcagatg ctccttgaag gcttgttcgc cttcacaggt cctggaatat gttgtccggg
     8041 gtttccgttc gataaattcc tttttcactt caatgtaccc caatccttca agcgttttga
     8101 ggtggcttgc cagattgcca tcggtgaggc ccagcatttc cttgaacgta ttgaaatcca
     8161 cctcattatt caccatcagc atcgacatta tgcccaggcg taccttgctt tcgaaagcct
     8221 tgttaatatc ttcgagaaat tccttcacga taacctttct tttctttcga tgcttacgta
     8281 gaaataaatt ccgtaaagga tgtgcaagac tccgaaaccc gcagaccaga acaacagccc
     8341 ttttccggga aagaatgcac acagcaggcc taaaactatc tctgcgcagc ccaacccgaa
     8401 aatacttccg taggtgtatt tggcggcatt gatcaacgaa acaccgtaga acagcaacat
     8461 gactggtgca acaatcccga cgtatccatt gagaagcagg gccaggcaaa aaatacctcc
     8521 ggtagtaagc ggcaggaaaa agttgaaaaa tgtccggtaa gccggtttgt tgaaaaaaga
     8581 ctgctgcctc ttttttgctt tccgtaccga gaaaaaaagg atcgtcccca atgccaggac
     8641 aaacacaagc agtgccgtaa taataagttg tggcgcaaca gcaagccaca gcggagcgga
     8701 ataaccgtcc ggcacggagt taaccgaagt cagtacactc cgggcaatcc atgacccccc
     8761 caacgcgtac aaaccgataa ggatggcgga agtgccgctg aaagagataa acctcgacga
     8821 cttctccatc atcgccctga tctcttttac agtttctatt gcttcaatat ttttcataat
     8881 aaaaagtact ttgaactaca aagtaaagga taaaaaatta aagccggcac aaaatacacc
     8941 gactttaact tagtttaatt gtttttgtag cggctgacca actcgattga aaccggtccc
     9001 ataagcccat actcctttag cggggctttg gcttcgggcc ccgcaatggt ccatgttctc
     9061 ctgctttctt caggcaaccc ggcatcgtac accaaccggt tgaaccaggt actggttacc
     9121 tccacctgca aattattctc tccttcctgc agataccggg taacatccag cacgtacggt
     9181 gaggtccagc aagtaccaac ttcctggccg tttatcctta ttctggcgat aacctccacc
     9241 cggcccaaat tcaagaggta cgccgttgtt ccagacttac ggtctaacgt aaaggtggtc
     9301 tcataggtag ccgtcccgga gaaagatttc ccttcttccg ataccgggaa atctttccag
     9361 gcaatcagtt tttgggtaac caccggtgag ttttccactc cccaccctcc gggaaaataa
     9421 acttgccagg atgcctggct taagtccaac ttttccgttg gttgcggctg tctaagcggc
     9481 ttattcgatt ttctattttt aaaaacgaga aaacaggagc ttccggccgg aatatccagt
     9541 tcaacctccg tgtaattatc ctttacgttg gaatttaccg caacgatttc cccggataac
     9601 ggatcccaaa gttccgcatt tccccggata cgaaaagaag ccaagccccg gtaccccttt
     9661 tcaggagagg ttgagaggaa atagatgccg gactcaccag tttggcggtg cagccagcgc
     9721 aggtcggggg aatgggcaag aatgtccggc gggatatttt ccgctgtcag aacggtagct
     9781 atatctgatg tattgtaaac tttccctttc cccagggtaa gcatgttttt cgagccgtct
     9841 ccccacagtt ttccgacggc tttgtcgaac ctcagttttg tttcatcccc accgcttaag
     9901 gtagctatac ccgacggggc atctccggca agaatcaccc ccgcttccac gaacgaagcg
     9961 atcttttcta acgtttccgg cagcatcctc ctgcaatcgt acaaccacaa cactcggtac
    10021 tggattcctt caggagtgac cagcttgcca ttcttaaccg acaagcggtt caacaaaata
    10081 tcgggattgc aataatcgta ggaatatcct tcggggaaag gaagtaactg gggcggctta
    10141 tgattttgtt cgtctcccag atacatcaat acgtccgata ccgggtttcc cgtttccagc
    10201 atgtaattac acctcgccag gtaatccgtg aacaggggca tgtgctgcca ccaggtctgt
    10261 aagcgcagga acggcgtacc gatctttgtt ccgaacgatg tgccgggagg caggaaatcc
    10321 gtacgcgggt tatgagtata agtatggaaa accagatggg tcaccccttt ggcaaaatgg
    10381 tcatcggcaa tgtctttcag gaaacgggga tgttcatccc aagtaaggtt gaaagaggtg
    10441 aacgcttctg ccgccaccct cttttttccg taaccccgcg cggcagagac ggccggtctg
    10501 accggcttga attcaatgct ccccacaaac gaatccgagc gtggctgcca gaattcacac
    10561 atgggaacat cggcatgttt gtagtattcc agaatatctc cgggaaaaac atcacccgat
    10621 gccgtctcaa aagagaccgt caatccgttt tcatcggcca acttttttat ctccccaaaa
    10681 aagttttcca ccagcagatc gttgagggtc acgcgccagt cacgaagaaa ccgcgctgta
    10741 ttctccggat tatccaccac atacccgaac aaggcaggca acctggagcg aagggcgtaa
    10801 ctccattgat tatcaaatat tttatcgaga tcggccgtcc aggtctgcgt cttgcattcc
    10861 caactatcca gcaggacgcc ttgcagcaat ccgtctttca gcgctccatc tcctttaatc
    10921 aaacgcccga cgtacccatc aaagttcgct cttataccgc tagggtgaag tttggttgcc
    10981 tcccagcccg ttgcttcggg aggggccggt ccgtttttct ggcccgtatt gacatgtcct
    11041 atacgaagaa cggtccagtt ccccgcagga acagtccagt taagttttcc tgaagaatcc
    11101 atatgattgg acatgtccac aatcgtggag ggatcaaccc aggagcgggg atcctgttgt
    11161 ggatattcct ccctgacaat ccttctcaac gtccacgcgg cttctgactc ccagttctgc
    11221 tgacgggcag cagaataaaa atggatgtac ggcaaagtca tttcatgcag gttggaaatc
    11281 tcaatccggt aagaatcgga aggtgtctct gcacaggcaa tcgatagggg ttggtcgtcc
    11341 tgccagtttg cagccggcat gtcaataaat gccacccggc gtctttcacc gggcaggttg
    11401 gcgtaaacgg ctacctgtac acccggtgca tacacccggg catgagaata ggagtctatg
    11461 ggcgggaatt caactgttcg caaggtaatt ttttccggaa acgtgatatt caggacagta
    11521 ggttcatcca ccgaaggagg gatcgtgagg gtcttccggt ctaccaggca atcgcgccaa
    11581 gacaagccga agcgattgct tgtgattctg gaagggatca accgtgcgcc agtatcacct
    11641 tcgggtgttg ggaacgcgat cacaaacaaa tcccggtaat cgcgccactc ttcctctatg
    11701 ttcccgggct ttgccagggt tatctccgac gcgacacctc cggccaggtc tgtccgcgaa
    11761 tataccaggt gccgcatg
//
LOCUS       DAOSDR010000035        11744 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrimonas sp. isolate AMR_MDS_1826
            NODE_2107_length_11744_cov_3.588160, whole genome shotgun sequence.
ACCESSION   DAOSDR010000035 DAOSDR010000000
VERSION     DAOSDR010000035.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863355
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrimonas sp. (wastewater metagenome)
  ORGANISM  Petrimonas sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Dysgonomonadaceae; Petrimonas.
REFERENCE   1  (bases 1 to 11744)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11744)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:10:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,720
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 35
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11744
                     /organism="Petrimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2107_length_11744_cov_3.588160"
                     /isolate="AMR_MDS_1826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2023866"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            13..669
                     /locus_tag="PLI30_02470"
     CDS             13..669
                     /locus_tag="PLI30_02470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008659460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HOI78449.1"
                     /translation="MKKILLLCFITCTTATTIAQQRNHSTFYHQRASLFEKLPVTEKD
                     ILFVGNSITNGGEWAELFNDPRIKNRGISGDICEGILDRLAPLLRGKPAKIFLLIGIN
                     DLARNTSPDTVVSMTADIVNRIRCESPGTAIYLQSILPVNDCYGMFEGHTRHGRAIAS
                     LNERLKEIATSQSIIFVDLYSHFIIPGSDKLDPRYSNDGLHLMGEGYLKWVEILKPYL
                     "
     gene            682..2760
                     /locus_tag="PLI30_02475"
     CDS             682..2760
                     /locus_tag="PLI30_02475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009860533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HOI78450.1"
                     /translation="MKKSILFVLFLIPTLLFAERTKVACVGNSVTYGYKIDDRAFTYP
                     AQLQQLLGNTYRVENFGHSGATLLQKGHNPYRKLKVFEQALAFEPDIVVIHLGLNDTD
                     PRNWPKFRDDFTRDYIDLIRSFQEKNPATKVWICRMTPIFSWHPRFKSSTRVWYEQVQ
                     QEIERVAAVTRVPLIDLNRPLYNRPDLFPDALHPTTEGAGIIARTVYGAITGDYGGLQ
                     MPEIFTDHMVMQRNRPVKFWGTADAATKVTVCFAGKTSTAQTGHNGKWTVEFPPMKAG
                     GPYNATIENQGRSIRLTDILVGEVWICSGQSNMAFRLQDASTAQKDIPRAANRQLRFF
                     DMKEIAPTVAIEWNDNVLAEVNRLGYYRPAAWALSDSLTARQFSAVAYYFGRMLQEEL
                     NVPIGLIQNAIGGSPTEAWIDRRTMERDPLMADFFTNWNSNDFIDGWVRERGMQNIAR
                     AKENPLQQHPYKPSYLFEAGMASISKYPVAGAIWYQGESNANNVELHGQLLPILVESW
                     RNAWEERFPFYYVQLSSMETGRESWGHFRDSQRRLMERIPRSGMVVSSDLGNRTDVHP
                     RQKKEVGERLARWALADSYGKNILKSGPLFREVFFEKEKAVVTFSETKLLRTSDNNAV
                     RNIELAGEDKVFYQAKAMIRANRLEVTCEKVSRPAFVRYGWDSYTEGNLVNEAGLPAS
                     TFSSEFQDKK"
     gene            2874..4727
                     /locus_tag="PLI30_02480"
     CDS             2874..4727
                     /locus_tag="PLI30_02480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002560862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 20 glycosylhydrolase"
                     /protein_id="HOI78451.1"
                     /translation="MLTIDLSGQHFLRLGVPDSSLVSEKLVNAIPEATINPDEAYRLR
                     ITPDSILIEATTEKGIYWAWQTLAQIVESSDGNSVPALEITDWPAFRIRGFMHDVGRS
                     YISVDEIKKHIRLLSKFKINVFHWHLTENQGWRLESNVFPQLNDPVHYERHHAQYYTV
                     AQAHEIAEYCRQHNMLLIPEIDMPGHSAAFVRAIGHDMQSPEGMKVLKRLMEEICTEV
                     FPDAPWIHIGTDEVQFTNPSFVPEMVAHVRGLGKKVISWNPGWAYRSGEIDATQLWSY
                     RGKAQPGIPAIDSRFHYINHFDAFGDIVALYNSRIADAEKGSDDIAGGIVAVWNDRLL
                     PNDEQIVLQNNFYPSMLAFAERTWCGGGSEYFDKNGTILPTDENDAVFNGFVDFEKRM
                     LWHKKHTFTDEPFAYVKQTNVKWRITDAFPNGGDLLKSFPPETGIDDTYEYNGIGYGS
                     HNATGAGIYLRHVWGKTVPAFYKNPQENHTAYAYTWVWSPKAQTVGLWTSTQDYSRSE
                     SDLPPPRGKWDYRESRIYVNNNEIMPPVWENTHTGRTNEITLKNENFQARPPIPVELN
                     KGWNSVLLKLPVGTFSSSGVRLQKWMFTFVFVTPDGKDAVEELVYSPDRKK"
     gene            4839..5948
                     /locus_tag="PLI30_02485"
     CDS             4839..5948
                     /locus_tag="PLI30_02485"
                     /inference="COORDINATES: protein motif:HMM:TIGR03548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclically-permuted mutarotase family protein"
                     /protein_id="HOI78452.1"
                     /translation="MKLKLTQLPVNGLYGDLAKGVSASYAALIDQKLIVAGGANFPGK
                     LGFEGGSKTFYNEIMLYDSTGNEWKLIGHLPDSSAYGVSVPISDGALWIGGNTTSQSL
                     KSVYRITMPETGAVELEPFPELPAAMDNFAGCTVGDTVFIGGGNENGKPANTFYCINT
                     KADSGWTALPDFPGLPRVQPVLAAVEQKNRVFVYLFGGFFGGDSENKPAMATDVLRYD
                     VAAEKWETAGCQVDEKTGEPFSLTGATAMPVDNRYILCFGGVNHAIFLDAVTTQYNIG
                     NDTTISAEEKSRLNLEFSKNYMTQPIEYYKFNPECRIFDTFTGEWETIEVTPYTARAG
                     ATLAFSGKTFYAVQGELKPGVRTPVTIKGEIKYEK"
     gene            5938..7224
                     /locus_tag="PLI30_02490"
     CDS             5938..7224
                     /locus_tag="PLI30_02490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019537699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOI78453.1"
                     /translation="MKNNKVYPWILVGLLWFVALLNYLDRQMLSTMQSSMQLDIKELA
                     IAENFGRVMGVFLLIYGLMSPVAGIIADRINRKWLIVGSLFVWSAVTYGLGYAQTFQQ
                     VYWLRALMGVSEALYLPTGLAMIADFHTSRTRSLAIGIHMSGLYTGQALGGFGATIAA
                     NYSWNTVFHWFGIVGIIYAVLLIFLLHDKEGHAGTTTAGLKADPQGTLIKKESVFRSF
                     GIILGTVSFWVMLFYFMAPSFPGWATKNWLPTLFSENLGIEMAKAGPMATISIAIASF
                     IGVLIGGPLSDRWVQKNIRGRIYTSTIGLALTIPSLVLLGFGHSYTGLIGAAMLFGIG
                     FGMFDTNNMPILCQIIPQKYRATAYGIMNMMGVFAGYAVTLMLGSSTDAGSLGTDFSK
                     LAIVVLAAVVLMLAFLKPRQDLTYPQEELKNKSEAI"
     gene            7266..8192
                     /locus_tag="PLI30_02495"
     CDS             7266..8192
                     /locus_tag="PLI30_02495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009039361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HOI78454.1"
                     /translation="MKKFEKIKGLIVAPFTPFDKKGEVDLGPIADYAAMLQKNGLIGV
                     FINGSSGEGYMLTVEERMKLTEKWIAVAPEGFKVIVHVGATCIKDSHRMALHAQESGA
                     FGIGAMASPFPKAGRVEELVKYCEEIACGAPELPFYFYHIPALNGVFLPMLPFLKAAD
                     GRIPNLAGIKYTYESLYEYNQCMLYKNGKFDMLHGQDETILSALVMGGAQGGISGTGS
                     YIGRSLVGVIDAYNNGKLYEAVAHQNFAQEVINVIARYRGNIVAGKRIMKLIGLDLGI
                     NRTPFQNIADEEEAVIRKELEAIGFFERCNRV"
     gene            8407..9018
                     /locus_tag="PLI30_02500"
     CDS             8407..9018
                     /locus_tag="PLI30_02500"
                     /inference="COORDINATES: protein motif:HMM:NF024895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin family protein"
                     /protein_id="HOI78455.1"
                     /translation="MKKTILACLSFLMAAGLLNVNAQTSVSYGVKGEMNMSNFLLKDL
                     DVQKSTMKVGPNLGGFMKVDLHPNFAIQPEVMFFYRNSKMETGPAEDTFKQWGMQIPV
                     YALGQTSTDNGRFYGGVGPFVSLGFDARYKDADVNLYDEVAGKTPMNRWDVGLGAILG
                     YELDNGIQLNAGYQFGFLNQLDALKDVAKMRTQAVCVGVGYRF"
     gene            9119..10243
                     /locus_tag="PLI30_02505"
     CDS             9119..10243
                     /locus_tag="PLI30_02505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004330860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HOI78456.1"
                     /translation="MKLRKRQPFFLNYNKKTALTAAIVISFILAFVMLSSSIYAISLD
                     KTMGIKAADLISVKSIATLLFNTLLLYFLFRLQFWAITHFPASRYKIWLILIGLLVII
                     AILSPFFSRMQWWWFREEVSPKAYSTIHYVKDLTVLIISFLFTALIYLINENQKNMTE
                     NQNLVIENLQNRYNALKNQTDPHFLFNSLNTLNGLIGYDDERAREYVEQLSAVFRYTM
                     QDRSVIKVSEELQFVESYIYLMKIRYNDGLNVHIQHAGLYQECYILPFGLQILIENAI
                     KHNVISRKDPLCILIEATENNTLRVENNRQPKQGEKNTTGLGLANLNERYRLMFGKEI
                     EIYSDDKVFAVEIPLIKNREIPDSVKQEIRTYDRTNSAVI"
     gene            10262..11029
                     /locus_tag="PLI30_02510"
     CDS             10262..11029
                     /locus_tag="PLI30_02510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019539113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HOI78457.1"
                     /translation="MKAVIVEDEFVAARSLERLIAASGRNIDIVAVLQSVEESVEWFS
                     LNTHPDVVFMDIHLADGDSFAIFEKTDVRCPVIFTTAYDEYALKAFEVNSIDYLLKPI
                     TRKSLEKALDKLAHFSPKSNNTDIVSQIVQSIREIKSPYKKNFLIPYRDKLIPLAAED
                     IAFVYSEFKMAKIVCFNRQSYSMDISLDEFLRQLNPSLFYRANRQYIVSHRAITDLSL
                     WFNGKLSVNLSVATPERIIVSRARSNEFKAWYMENAE"
     gene            11169..>11744
                     /locus_tag="PLI30_02515"
     CDS             11169..>11744
                     /locus_tag="PLI30_02515"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HOI78458.1"
                     /translation="MNSEKILLICFLTVFSLLAVPISAQQVLTLEECRKMALEQNKKI
                     RMAKEDAQMACLTKKAAATKYLPSISLNGGYLRTNKTLKPLGNDLFLPVVPFNAIDPQ
                     TGEFNPASLQDPATALNTLVINPQTGQPLLDASGNPVFKNYALLPSDKLVFGNKNSYY
                     TRLSVNQPLFTGFKITEANKIAQHAENIAKEN"
BASE COUNT         3238 a         2889 c         2828 g         2789 t
ORIGIN      
        1 gcatttaccc gaatgaaaaa aattctgtta ttgtgtttca ttacatgtac cacggcaaca
       61 accattgccc aacaacgaaa ccactccact ttctaccatc aacgtgcttc cctgtttgag
      121 aaactgccag taacagagaa agacatcctt tttgttggca acagcatcac caatggcggt
      181 gaatgggcag aattatttaa cgatccgcgc ataaaaaacc ggggaatcag cggagatatc
      241 tgcgaaggga tactggacag actggccccc ttgctgcgtg gcaaaccggc gaaaatattc
      301 ctattgattg gtatcaacga cctggcccgt aacacttccc ccgatacggt tgtgtcaatg
      361 actgccgaca ttgtaaacag gatcaggtgt gagtcacccg gaacagcaat ctacctgcaa
      421 agcatacttc ccgtgaacga ctgctacggg atgtttgaag gacataccag acacgggaga
      481 gccattgctt cgttaaatga gcgcttgaaa gagatagcga catcccaatc gatcatattt
      541 gtagaccttt acagccattt cataataccc gggagtgaca agctggatcc ccgatacagc
      601 aatgatggtc tccacctgat gggtgaaggt tacctgaagt gggtagagat tttgaaacct
      661 tatctgtaag tatatcccat gataaagaaa agtatactgt ttgttttgtt tcttatcccg
      721 acactgctct ttgcagaacg gacgaaagta gcctgcgtgg gaaactccgt cacctacggc
      781 tacaagatcg atgaccgggc attcacctac cctgcccaac tgcagcaact gctaggcaat
      841 acgtaccgcg tggagaattt cggacacagc ggcgccaccc tgttacaaaa aggccacaat
      901 ccctaccgga agctgaaggt atttgaacaa gcactcgctt tcgaacccga catcgtggta
      961 atccacctgg ggttgaacga caccgatccc cgcaactggc caaagtttcg ggacgacttt
     1021 acccgcgatt acatagacct gatccgttcg ttccaagaga aaaatccggc tactaaggtg
     1081 tggatctgtc gcatgacacc catctttagc tggcaccccc gcttcaagtc gagcacccgg
     1141 gtgtggtacg agcaagtaca acaggaaatt gaacgggtag cagcagtgac ccgtgtgccg
     1201 ctgatcgacc tgaacaggcc tttgtacaac cggcccgacc tctttcccga cgcactccac
     1261 cccacaaccg aaggagcagg catcattgcg cggacagtct acggagccat caccggtgat
     1321 tacggcggcc tgcagatgcc agagatattc actgaccaca tggtgatgca gcgcaacagg
     1381 ccggtaaagt tttggggaac cgccgatgca gccacgaaag taaccgtctg ctttgcggga
     1441 aaaacaagca ctgcacaaac cggccacaac ggaaaatgga cggttgaatt tcctccgatg
     1501 aaggcaggtg gcccttacaa cgctaccata gagaaccagg gaagaagtat ccgcctgacc
     1561 gatatcctgg tgggcgaggt gtggatctgc tccggacaat cgaacatggc ctttcggcta
     1621 caggatgcct ctacagcaca gaaagatatt cctcgtgccg ccaacaggca acttcgcttt
     1681 ttcgacatga aagagattgc acccacggtg gccatagagt ggaacgacaa cgtgctggca
     1741 gaggtgaacc ggctgggcta ttaccgtccc gcagcgtggg cgctgtccga cagcctcacg
     1801 gcaagacagt tctccgctgt tgcctactat tttggcagga tgttgcagga agagctaaat
     1861 gtaccgatag gattgataca gaacgctatc ggagggtctc ccaccgaagc gtggattgac
     1921 cgcagaacga tggagcggga tccgttgatg gccgatttct tcaccaactg gaacagtaat
     1981 gattttatcg acggatgggt acgtgagcgg gggatgcaaa acattgcccg tgcgaaagag
     2041 aatcccctcc agcaacatcc ctacaaaccc tcctacttgt ttgaagccgg aatggcatcc
     2101 atcagcaaat atcctgttgc cggagctatc tggtaccagg gtgagtctaa cgccaataat
     2161 gtggaactgc atggacaact actccctatc ttggtggaaa gttggcgcaa cgcctgggaa
     2221 gagcgcttcc ctttctacta cgtgcagctt tcgagcatgg agacggggcg tgagtcctgg
     2281 ggacatttcc gcgacagtca acgcaggctg atggaacgga ttcctcgctc cggaatggta
     2341 gtctcctctg atctgggtaa ccggaccgat gtccacccca gacagaaaaa ggaggtggga
     2401 gagcgtctgg cacgctgggc actggccgac agctacggaa aaaacattct gaaaagtggc
     2461 ccgctcttca gagaggtatt ctttgagaaa gaaaaagcgg ttgtcacttt tagtgaaaca
     2521 aagcttctcc gtacgtcgga taacaatgct gtaaggaata tcgaactggc aggagaagac
     2581 aaggtttttt atcaggcaaa agcaatgatc agggccaacc gtctggaggt gacctgcgag
     2641 aaagtgtccc gccccgcatt cgtacgctat ggctgggatt cctacacgga aggcaatctg
     2701 gtcaacgagg caggactacc cgcctccacc ttctcatcag aatttcagga caaaaaataa
     2761 ctatttcccc gtacatgaag atatttacat acatcgtgct gaccacccta ttgcttctct
     2821 cctgtagcac gacagagagg aaagaaacat ttcagctgtt gccggaaccg aagatgctca
     2881 ccattgacct ctccggacag cacttcctcc gtctgggagt acccgattcc tccctcgtct
     2941 ccgaaaaatt ggtgaatgct atccccgaag caactatcaa tcccgatgaa gcctatcgcc
     3001 tcagaattac gcccgacagc atcctgatcg aagccactac cgaaaaaggt atctattggg
     3061 cgtggcaaac gctggcgcaa atagtggaat catccgatgg gaactcggtt cctgctctcg
     3121 aaattaccga ctggcccgct ttccgtatcc ggggatttat gcacgatgtg gggcgcagct
     3181 acatatcggt tgatgaaatc aaaaaacata tccgtttgtt gtcaaagttc aagatcaacg
     3241 ttttccactg gcacctgacc gaaaaccagg gatggcggct ggagagcaac gtttttccgc
     3301 aacttaacga tcctgtccat tacgaacgcc atcacgcaca atattatacc gtagcccagg
     3361 cacacgaaat tgccgaatat tgtcgccaac acaacatgct gctgattccg gagatcgata
     3421 tgcccggtca cagcgctgcc tttgtgcggg ccatcggaca cgacatgcaa tcgcctgagg
     3481 gaatgaaagt actgaagcga ttgatggagg aaatctgtac cgaagtgttt cccgatgcac
     3541 cctggatcca tatcggaacc gatgaagtgc agttcaccaa cccctccttt gtcccggaaa
     3601 tggtggcaca cgtccggggg cttggcaaaa aggtgatctc ctggaacccc ggatgggcat
     3661 acaggtccgg ggagattgat gccacacagc tctggagtta ccgcggcaaa gcgcaaccgg
     3721 gtattcccgc gatcgactcc cggttccact acatcaacca cttcgatgcg ttcggcgaca
     3781 tcgtagcact atacaatagc cgcatcgccg atgcagaaaa gggatccgac gacatcgccg
     3841 gcgggattgt cgctgtctgg aacgaccgtc ttttacccaa cgacgaacaa atagtactgc
     3901 aaaacaactt ctacccctca atgcttgcct ttgccgagcg gacctggtgc ggtggcggga
     3961 gtgagtattt cgacaaaaac gggactatcc tgccaaccga cgagaacgac gcagttttca
     4021 acggttttgt cgatttcgag aagcggatgc tctggcacaa aaaacacaca ttcaccgatg
     4081 agccgtttgc ctacgtaaaa caaaccaacg taaagtggcg gattaccgac gctttcccga
     4141 acggtggcga tctgttgaaa tcgtttccgc ccgagaccgg aattgacgac acctatgaat
     4201 acaacggaat cggatacggt tcgcacaatg ccaccggggc gggaatttac ctgcgccacg
     4261 tgtggggaaa aaccgtacct gctttctata aaaacccgca agaaaaccac acggcatatg
     4321 cttatacgtg ggtttggtcg cccaaagcgc aaacggtcgg gctatggact tccactcagg
     4381 attacagccg ctccgagtcc gacctgccac cgccacgggg gaagtgggat taccgggaaa
     4441 gtcgtattta cgtgaataac aatgagatca tgccacccgt ttgggaaaat acccataccg
     4501 gcagaacaaa cgaaataacg ctgaaaaacg aaaatttcca ggcccgccca cccattccgg
     4561 tagaactgaa caagggatgg aacagcgttt tgctaaaact tcccgttgga acgttcagtt
     4621 cgtccggggt gcgtttacag aaatggatgt tcacgtttgt ctttgtgact ccggacggaa
     4681 aagatgcggt ggaggaactg gtatatagtc cggacaggaa aaaatagaat taacacatga
     4741 aagaaagcat tttttttaga acaaacagaa cacgaatact gtcggttttt cttgtcgcat
     4801 gcttaatttc gtgtggcgac aataataaca gcaaacaaat gaaacttaaa ctgacccaat
     4861 tacccgttaa cggattgtac ggagatctcg ccaaaggtgt ttcggcttcc tatgcggcac
     4921 ttatcgacca aaaactcatc gtggctggag gcgccaactt tcccggaaaa ctcggatttg
     4981 agggtggttc gaaaactttt tacaacgaga tcatgctgta cgactcaacg ggaaatgagt
     5041 ggaaacttat cggacacttg cccgattcat cggcttacgg tgtatctgtc cccatttcag
     5101 acggtgcgtt gtggataggc gggaacacca cttcccaatc gctgaagagc gtttatcgaa
     5161 tcaccatgcc tgaaaccggc gcagtagaac tggagccgtt tccggaactg cctgctgcca
     5221 tggataactt tgcagggtgc accgtcggag atacggtctt tattggagga gggaacgaga
     5281 acgggaagcc ggcaaacact ttttattgca tcaatacaaa agctgattcg ggctggacgg
     5341 ctctgcccga ttttcccgga ctgccccgag ttcagcctgt gctggcagcc gtagagcaga
     5401 aaaaccgggt attcgtgtat ttgttcggag gtttcttcgg aggagattcc gaaaacaaac
     5461 cggctatggc caccgatgtg cttcgttacg acgtcgccgc cgaaaaatgg gagacagccg
     5521 gttgccaagt cgatgaaaaa accggagagc ctttttcgtt gaccggtgca acggcaatgc
     5581 ctgtggacaa ccggtacatc ctttgttttg gcggtgtaaa ccacgctatt tttctcgatg
     5641 ctgttaccac gcaatacaac atcggaaacg acaccaccat ttcggcagaa gaaaaatccc
     5701 gtttgaatct ggagttttcg aaaaattaca tgacacaacc catcgaatat tacaaattta
     5761 atcccgaatg tcgtatattt gacacgttta ccggcgaatg ggaaaccatt gaggttacgc
     5821 cctacactgc ccgcgcgggt gcaacactcg ctttcagcgg aaaaacgttc tatgctgtac
     5881 agggtgaatt gaaacccggc gttcgtacac cggtaaccat aaaaggagag ataaagtatg
     5941 aaaaataaca aagtctatcc gtggatactc gtcggattgc tgtggtttgt agcgttactg
     6001 aactacctcg accgacagat gctctccacc atgcaatcgt ccatgcaatt ggacattaaa
     6061 gaactggcca tcgccgagaa tttcggacgg gtaatgggtg ttttcctgct catttacggt
     6121 ttaatgagtc ctgtagcggg aataatcgcc gaccgcatca accgcaagtg gctgattgta
     6181 ggaagcctgt ttgtgtggtc tgccgtaacg tacgggttag gatatgcgca aactttccag
     6241 caggtgtact ggctgcgtgc gctgatgggt gtcagcgaag cgctttacct gcccacggga
     6301 ttggctatga ttgccgattt tcacacatcg agaacccggt cgctagctat cggaatccac
     6361 atgtcgggac tttataccgg ccaggcgctg ggtgggtttg gagcgaccat tgctgccaat
     6421 tattcgtgga ataccgtttt tcactggttc ggaattgtcg gaattatata cgccgtgctg
     6481 cttattttcc tgctgcacga caaggagggc cacgcgggta ctacaacagc cggattgaaa
     6541 gccgacccgc aaggaacgct aatcaaaaag gaatctgttt tccggtcatt cggcattatt
     6601 ctgggtacag tttctttctg ggtcatgctt ttctacttta tggctccgag ttttcccggt
     6661 tgggcaacca aaaactggct gccgacactg ttttccgaga atctgggaat tgaaatggca
     6721 aaagccgggc ccatggccac catttccatc gctatagcct cgtttatcgg cgtgctcatc
     6781 ggagggccgc tctccgaccg atgggtgcag aaaaacatcc ggggacgtat ttacacgagc
     6841 actatcggtt tagccctgac tatcccttca ctcgtattgt tgggcttcgg acattcctac
     6901 acaggattga tcggcgcagc catgctcttc ggcatcggtt tcggaatgtt cgacacaaac
     6961 aacatgccga tactttgcca gattattccg caaaaatacc gcgccacagc gtacggtatc
     7021 atgaacatga tgggagtttt tgccggatac gctgtcacgt tgatgctcgg tagttccacc
     7081 gatgccggaa gcttgggaac cgatttcagc aagttggcca tcgtcgtact ggcagcggtc
     7141 gttctgatgc tggcgtttct gaaaccccgg caagacctca cctatccgca agaagaattg
     7201 aagaataaaa gcgaagcgat ttgacaactg ttatctaaca ataaaacatc ctaaaaatca
     7261 acaatatgaa aaaatttgaa aaaattaaag ggttaattgt ggcgcctttc acaccctttg
     7321 acaaaaaagg cgaagtagat ctcggcccca tagcagatta tgccgccatg ctgcaaaaaa
     7381 acggactgat cggcgtattt atcaacggtt cctcgggtga gggctatatg ctgacggtag
     7441 aggagcgtat gaagctgacg gagaagtgga tcgcagtcgc cccggaagga ttcaaagtaa
     7501 tcgtccatgt tggggccacc tgcataaagg acagccaccg catggcactg cacgcgcagg
     7561 aatccggcgc tttcggtatc ggagcgatgg cttctccttt tcccaaggcg ggacgggtgg
     7621 aagagctggt gaagtactgc gaagaaatcg cctgcggtgc accagagctg cccttctact
     7681 tttatcatat cccggcactg aacggcgtct tcctacccat gctgcctttt ctgaaagcag
     7741 ccgacggacg cattcccaac ctggcgggta tcaagtatac ctacgagagc ctgtacgagt
     7801 ataaccaatg catgttatac aaaaacggga agttcgatat gctgcacggc caggacgaaa
     7861 ccatcctctc ggcgttggta atgggcggtg cacaaggcgg tatcagcgga accggcagct
     7921 acatcggccg ttcgctggtg ggtgtgattg atgcctacaa caacggtaag ctgtatgaag
     7981 cggtcgccca ccaaaacttt gcacaggagg tgatcaacgt cattgcccgt taccggggaa
     8041 acatcgttgc cggaaagcgt atcatgaaac tgatcggact cgacctgggt atcaaccgca
     8101 ccccttttca aaacatagca gacgaggaag aagcagtcat ccggaaagaa ctggaagcga
     8161 tcggtttttt cgaacggtgc aacagagtgt aagtttaaac gttaaaaagc gcggctcatc
     8221 gatgtcgcgt ttttttattg acgtcataag ccggccatcc gccggtaaca aatattcttt
     8281 cgggttcttc cttctactgt tcagcaccta aaatagacgg ttgagtatct ttttcggggc
     8341 agtcatcttt gttgtacgct acctttgcaa gcgagatagt ttataacaat ttaaaacaac
     8401 agaaaaatga aaaaaacaat tttagcatgc ttgtcatttc taatggctgc aggtttattg
     8461 aatgtgaacg ctcaaacctc cgtttcttat ggggtaaaag gcgaaatgaa catgtctaat
     8521 tttcttttga aggatttaga cgttcaaaaa agcacaatga aagtggggcc taatttggga
     8581 ggctttatga aagtggactt gcatcctaac tttgccattc agccggaagt gatgtttttt
     8641 tacaggaatt cgaaaatgga gaccggtccc gctgaagaca ccttcaaaca gtggggaatg
     8701 caaatcccgg tatatgcttt gggacagact tccacggaca acggcaggtt ttacggaggt
     8761 gtagggccat tcgtaagcct cgggtttgat gcccgctaca aagatgctga tgtgaatctg
     8821 tacgacgagg tggccggtaa aactccaatg aaccgctggg atgttggcct gggagcaata
     8881 ttgggttatg aattggacaa tggaattcag ttgaacgccg ggtaccaatt cggatttttg
     8941 aaccaattgg atgcattgaa ggatgtagcc aaaatgcgta cacaggccgt atgtgtaggc
     9001 gtgggatatc gtttctaaac tccgcaagaa tccataccgg taaaaaatgg tgcgaaaatt
     9061 tttaaattgc atacgcacca ttttttactt acatttgaag ttacaaacaa gaagacgtat
     9121 gaaacttcgg aaaagacaac ctttcttcct caactacaac aagaaaacag cgttgacagc
     9181 tgcaatcgtt atcagtttta tcctggcatt cgtcatgctt tcttcgtcca tatatgcgat
     9241 atcgttagac aaaacaatgg ggattaaagc cgccgacttg atttctgtca agtcgatcgc
     9301 aacgctcctt ttcaacacac ttctgctcta tttccttttc cggcttcaat tttgggcgat
     9361 aacacatttt ccggccagcc ggtacaaaat ttggcttatt ctgataggat tgcttgtcat
     9421 aatcgctatt ttgagtcctt ttttttcccg gatgcagtgg tggtggtttc gggaggaggt
     9481 atcgcccaaa gcctattcca ccatccatta tgtaaaagat ttaacggtgc ttatcatatc
     9541 ttttttgttc acggctctga tttacctgat caatgaaaat cagaagaaca tgactgagaa
     9601 tcagaacctc gttattgaaa atctgcagaa ccggtataac gcccttaaaa accaaaccga
     9661 cccccatttc ctcttcaact cactgaacac gttgaacggg cttatcgggt atgacgatga
     9721 gcgtgcgcgc gaatatgtag aacaactgtc tgccgtgttc cgttatacca tgcaagaccg
     9781 ttccgtgata aaagtttcgg aagagctaca gtttgtggaa tcctatattt acttgatgaa
     9841 aatccgttac aacgacgggt tgaatgttca catacaacac gcaggacttt accaagagtg
     9901 ttatattctc cccttcgggt tacagatatt gatagaaaat gcaataaagc acaacgtgat
     9961 aagccgaaag gatccgctat gcattcttat tgaagcaacg gaaaacaata ctttacgtgt
    10021 tgagaataat cggcaaccca aacaaggcga aaaaaatact accggtctgg gattggctaa
    10081 cctcaacgaa cgttaccggt tgatgttcgg gaaggaaata gaaatatatt ccgacgataa
    10141 agttttcgcc gtggagattc ccttaattaa aaacagggag atccccgact cggtaaaaca
    10201 ggaaataagg acttatgatc gcaccaacag tgccgtgatc taactctgaa cttttaattc
    10261 aatgaaagct gtaattgtag aagacgaatt tgttgctgca cgtagcctcg agcggcttat
    10321 cgcagcatcg ggaagaaaca tcgatattgt tgccgtgctg caaagcgttg aagaaagtgt
    10381 agaatggttc tcgctgaata cacaccccga tgtcgtattt atggacattc atcttgccga
    10441 cggagactct tttgcaattt tcgaaaaaac cgacgtccgc tgtccggtta tcttcacaac
    10501 agcttacgat gaatatgccc tgaaagcatt cgaggtaaac agcatcgatt acctgctcaa
    10561 gcccatcacc cggaaaagcc ttgaaaaggc gttggacaag cttgcccact tttctcccaa
    10621 aagcaacaat accgacattg tttctcaaat cgtacaatcc attcgtgaaa taaaaagccc
    10681 ttacaaaaaa aacttcctta ttccttatag agacaaactt ataccgcttg cggcagaaga
    10741 tatcgcgttt gtgtattccg aattcaagat ggcgaaaatt gtatgtttta accgccagtc
    10801 ctattcaatg gatatttccc tcgatgaatt tctccgccag ttgaatccgt cacttttcta
    10861 tcgggccaac cgtcaataca ttgtctcgca ccgcgccatc accgacttat cgttgtggtt
    10921 taacggcaag ttatcggtaa acctttcggt agcaacaccc gaacggataa ttgtaagccg
    10981 cgcgcgaagc aatgaattta aagcctggta tatggagaat gcggaataaa aaagcttcgc
    11041 ttcggtatct tcttactacg tttcatcccc taaaaagtac acttgggtaa ttttttccca
    11101 aaaggccacg caacagagca atacctttgt agccggtttt tgaattaaat tacaaacaga
    11161 tgaaaaaaat gaattcagaa aaaatactgc ttatctgttt cctcaccgtt ttttcactcc
    11221 tggccgtgcc aatctccgca caacaagtgc tgacgctgga agaatgccgg aaaatggcac
    11281 tggaacagaa taagaaaatc cgtatggcaa aggaggacgc tcaaatggca tgcctgacaa
    11341 aaaaagccgc agccaccaaa tatcttccca gcatcagcct taacggagga tacttgcgaa
    11401 ccaacaaaac attaaaaccc ttagggaacg atctgttcct gcctgtagtg ccgttcaacg
    11461 ccatcgaccc gcaaacggga gaattcaatc cggcatcgtt gcaagatccg gcaaccgcat
    11521 taaatacact ggtcattaat ccacagacag gccagcccct gctcgatgct tcgggaaatc
    11581 cggtgtttaa aaattatgcc ttgctgcctt ccgacaaact agtgtttggc aataagaaca
    11641 gttattacac ccgactgagt gtgaaccagc cgctttttac aggatttaag attaccgaag
    11701 ccaacaaaat cgcccagcat gcggaaaaca tagcaaaaga aaat
//
LOCUS       DAOSDR010000036        11734 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrimonas sp. isolate AMR_MDS_1826
            NODE_2110_length_11734_cov_4.544396, whole genome shotgun sequence.
ACCESSION   DAOSDR010000036 DAOSDR010000000
VERSION     DAOSDR010000036.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863355
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrimonas sp. (wastewater metagenome)
  ORGANISM  Petrimonas sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Dysgonomonadaceae; Petrimonas.
REFERENCE   1  (bases 1 to 11734)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11734)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:10:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,720
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 35
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11734
                     /organism="Petrimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2110_length_11734_cov_4.544396"
                     /isolate="AMR_MDS_1826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2023866"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..594)
                     /locus_tag="PLI30_02520"
     CDS             complement(<1..594)
                     /locus_tag="PLI30_02520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019538234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOI78459.1"
                     /translation="MNTSKKSNVLAPIAAFFGFFIMGFVDIVGIATNYVKNDFNLSSG
                     IAGTLPMMVFLWFAIFSIPTGILMGKAGRKKTVLLALATTCVAMIIPFLFYTFYTVLI
                     AFILLGISNTILQVSLNPLVAAMFNKEKTASILTLGQFIKSVCSFFGPIVAGFTASRF
                     GDWKLTFAVFAAISVISVFLLAFSKVEELGFKSTPATF"
     gene            complement(599..2347)
                     /locus_tag="PLI30_02525"
     CDS             complement(599..2347)
                     /locus_tag="PLI30_02525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009038778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I mannose-6-phosphate isomerase"
                     /protein_id="HOI78460.1"
                     /translation="MNRKRKSNYDKRPAVTIGKGFILEGWKSISSFVSGKVEHAKLLT
                     VDCYTGVYYDLLKSKLQEINNARLIDTLEIFKSENEINALTEQFLTEDILFGFYSNLS
                     ISDYFDEEKLRFARESIEQASGTVIVFGYGAQYVTTGDINIYADMPRWEIQQRMRRKE
                     VNGLGVNNKEETFSLQYKRGLFNDWKVLDKHKKTIYDTVDFWLDTVKVDLPKLIDKET
                     FFKGMDKAASSPFRLVPFFDPAPWGGQWMKEVCDLDRSVSNFGWCFDCVPEENSLLLK
                     INDIEFEMPSVNLVYTRSRELLGDPVEARFGKEFPIRFDLLDTIGGGNLSLQVHPTAE
                     YARENFGLAYTQDESYYIMDTKEDTYVYLGLRNDINPDEMVADLNKAQKDPFFVFDVD
                     KYVNKVPVKKHDHFLIPGGTVHCSGKDTLVLEISSTPNLFTFKLWDWQRLGLDGKPRP
                     INVDRGKKVIVWGRDTGFTYRELVNKVKILNDTGTVREEKTGLHKNEFIETRRHIFTE
                     KVTHYTNDSVNVLNLVEGDSVIVESLNGDFEPFVVHYAETFVVPARIKTYTVRPYGNS
                     KGNECITIKASVRFNA"
     gene            complement(2474..3229)
                     /locus_tag="PLI30_02530"
     CDS             complement(2474..3229)
                     /locus_tag="PLI30_02530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006797843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HOI78461.1"
                     /translation="MKLKIDRNSPVPLHKQAEMLLRALITKEEYKEGKMLPNEVELSE
                     QLKISRNTLRQSINTLVTEGLLIRKKGVGTRVAEKNLYSEATNWLSFSQEMKVLGVKI
                     ENFELHISKQAPTENARQFFEITNQVKVLRLERLRGKENFPFVYFHSEFNPKIRMDGT
                     ENFNRPLYEILENDYNIIVKTSREEIRASGANPFIAGKLEIEVGDPILIRKRFVSDIN
                     DLPIEYNIGWYRADSFSYKIESVRESRSFSSNF"
     gene            complement(3317..4801)
                     /locus_tag="PLI30_02535"
     CDS             complement(3317..4801)
                     /locus_tag="PLI30_02535"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HOI78462.1"
                     /translation="MKKLLLAGSFIFIYCLLLQSQSAYTLTLEESIEIAKKKSYSMLR
                     LSEDLKIAEFNLKSATSRLKTHIDMTLGAPQYNETIESWKDTTGITYYYPVKELGYSG
                     VLTINQPLPTDGNIFINNMLSSSEDLKNNFRSSRLRSRIGFNQPLNSFYAYNSIKSTL
                     KRAELDYERSSKQFKREELNLIYQVSSSYYNLLSLQRSTEIASLDLERQMEAHEISKN
                     KFAAGLIREVDALQMEVDLAEAQNNHDIAVLNEVSARNSFKELLGLDLNDSIALNNQL
                     NYQIVLIDPAKAVELALKNRLEVREQEIQIELQKLNIKQQKANGLIKGSVDAYFERAG
                     IDHRANISFMQSIKNSYNNLVDRNSSYGIGLTVTIPILDWGENRALVRAAESRLKQYN
                     YRKEEVEREIETEVKNLIASINSNLKRLQVLEKNVLVAEKSFEITRQRFSDGDIDSQG
                     LALERNRLNNAYTSHLRAYINYQLSLADLMRKTFYDFRNQREVE"
     gene            complement(4798..5529)
                     /locus_tag="PLI30_02540"
     CDS             complement(4798..5529)
                     /locus_tag="PLI30_02540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008862288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOI78463.1"
                     /translation="MLIEIKDLKKNYLLGDAEIPVIHGINLNIEKNEYVAIMGPSGSG
                     KSTLMNILGCLDTASSGHYYFNDVDVSTLDDDALSIMRNKEIGFIFQNFNLLPRLNAI
                     QNVELPLIYAGTPSAERKERALRALDRVGLSDRVTHKPAELSGGQRQRVAIARALVTT
                     PGILLADEPTGALDSKTGIEIMRLFEELHAEGNTIILITHEQEIASYAHRNIYLKDGM
                     IHSDKPNQKRTSLPSLNTFQNDEII"
     gene            complement(5545..6783)
                     /locus_tag="PLI30_02545"
     CDS             complement(5545..6783)
                     /locus_tag="PLI30_02545"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOI78464.1"
                     /translation="MQYGENIRLALQGLVDHKFRSFLTMLGIIFGVASVITMLSIGEG
                     AKREAIAKYQDLGVNNIIIREKTLSDEELEEVRAKFSQGLSIQDAQVIQEIVPGVERI
                     ASQAEKSTDVRYNDKSVKSTIIGITPDFQAMMNYKVRNGYFINDKHYNERLKVCVLGA
                     GVAAGFFKGEDPIGKLVKIDDQWLEVIGVLESKSLFTETVGELAARDLNTDVFVPLSL
                     FLNRFTRENALSSEIQQITVQLKNSDKLVEASKIINEILRRHHFNNDDYSIVIPYELL
                     KQEEKERQIYNFLLGAIAAISLLVGGIGIMNIMLATVMERTREIGIRRSVGARKIDIM
                     SQFVTESVAISITGGIIGVLTGIVLSLIVSLFTDINTFIKPYSVFIAFSFSVIVGVSF
                     GYLPAKNAANLKPVDSIRYE"
     gene            complement(6792..7793)
                     /locus_tag="PLI30_02550"
     CDS             complement(6792..7793)
                     /locus_tag="PLI30_02550"
                     /inference="COORDINATES: protein motif:HMM:NF027890.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HOI78465.1"
                     /translation="MKRSQLYSLFSVVLVAALLFSCKRSSESESERRTSDGSVIVETG
                     ELAAVNSRSVVVSRYGRQWYQMKIVGLLNHGEIVKKGDSIAQLDPADINKYILESESN
                     LETQLAVLEKLYVEQENKKQESESKIRNESAAFDLKKIEWEASRFESERLRKIKQLEY
                     EQAVINLEKEKKIYELNKAINTSELKIQEIRVEQIKKEVENAKNLIPLLTLRAPVSGV
                     FQIATNWQNSNRNIYKIGDNIYAGNNLAIVPELEFMKVNTQVNETDFLKVALNQKVAV
                     RLDAMPKVVFEGEIVYIGKLCRLKDQKSRQKVFDVEVKIIKPDERLKPGMTVSCEFL"
     gene            complement(7793..9034)
                     /locus_tag="PLI30_02555"
     CDS             complement(7793..9034)
                     /locus_tag="PLI30_02555"
                     /inference="COORDINATES: protein motif:HMM:NF027890.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HOI78466.1"
                     /translation="MKNFSKTWILIVICSLLFVACKKKETQQIPVGKVTQGTLFLDLY
                     EEGEIEAIKSINIVAPMISWRYGNLKITELVKDGQEVKAGDTLIVFDPSEVLKGIVEA
                     ESSLEIARAEFDKMKAQQQSELEELKAAYEVTRISHEISKIRFESAGYESDIKKKEIQ
                     LNLDKAEIALERAKEQIENRIKIQKEEIKQKNLSIMQFQSRLDEAHETLQKLSVVTPS
                     SGIAILSKNWSSNNKFQIGDQCWSGFPIIQLPDLSSLKATVKINEVDVAKISKGLKVE
                     IKPDAFSDSVFSGTVNSIANLAVNKDESSKIKVFPVEILLNESDKNLLPGLTVSCRII
                     MDKVEDVLYIPLDALFTEGDNNFVYKKKGTGYEKAEIETGINNTDYIVVVNGLKEGEE
                     IALVNPFSKETEEKKTENAEI"
     gene            complement(9055..10140)
                     /locus_tag="PLI30_02560"
     CDS             complement(9055..10140)
                     /locus_tag="PLI30_02560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI78467.1"
                     /translation="MIDGVAEPVRYTTIACPSNVDAIITFLIDDGTYVNEGDVVCILE
                     DNDRKTLHDNLAMQLETAVAELSKVKADLQMQYALLEAQVKNNKADTEIAQLDSLQLH
                     FATPTQKRIKELELERALIEKNKFEKKLEALEIINQSEVRKLELNIQQLTNRLASAKT
                     ILDGLTIRSPHKGLAVVSNSRMTGEKLKVGDNVWNNMPLITLPEVSEMKVKMMAGEVD
                     FRQINENDSVSFSFDAMPNNVAYGKITKKLPVGQPYKRDSKVKFFEVEASVDSALQLP
                     EPGFTANCRVFIKQIRDTIVVPQIAIYEEDSLKVVYVKKKNKYEMRPITTGLSSSKEA
                     IVSDGLKPGEVIALIKPPPSMVRGKTK"
     gene            complement(10389..11027)
                     /gene="pdxH"
                     /locus_tag="PLI30_02565"
     CDS             complement(10389..11027)
                     /gene="pdxH"
                     /locus_tag="PLI30_02565"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006798518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine-phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase"
                     /protein_id="HOI78468.1"
                     /translation="MKKLFEFREEYTTGELNETEVKANPMEQFQQWLQAAIDSGVPEA
                     NAMTIATCTASGKPSARVVLLKEINKNGFVFFTNYLSRKGRELLENPFAALVFDWHSI
                     ERQIRVEGRVEKLSTLDSDVYFNERPREAQIGAWASPQSKILGSREELEQRQASFEKK
                     FNDLEIFRPSYWGGFILLPTTIEFWQGRPGRLHDRLVYYRTEEGWTLHRLAP"
     gene            11237..>11734
                     /locus_tag="PLI30_02570"
     CDS             11237..>11734
                     /locus_tag="PLI30_02570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004309659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOI78469.1"
                     /translation="MIKVEKIQKSFGDLQVLKGIDLEVGKGEIVSIVGPSGAGKTTLL
                     MIMGTLEKANSGKVMIDNNDLSKLSEKKLADFRNKNIGFVFQFHQLLPEFTALENVMI
                     PALIGNMKQGAAEKKAKKLLAMMGLSERMEHKPAELSGGEKQRVAVARALINDPVVVF
                     ADEPSG"
BASE COUNT         2893 a         2566 c         2466 g         3809 t
ORIGIN      
        1 aaatgtagca ggagtgcttt tgaacccaag ctcctctacc ttggaaaatg caagcaagaa
       61 tacggaaatc acggagattg cagcaaagac tgcaaacgtt agcttccaat ccccaaaccg
      121 gctggctgta aatcccgcaa caatagggcc gaaaaaggaa caaacagatt tgatgaactg
      181 tcccagtgta aggatactgg ctgtcttctc cttgttgaac atggctgcta caagcggatt
      241 aagtgatact tgcaaaatag tgttgctgat acccagcaga atgaaggcaa tcagtaccgt
      301 gtaaaacgtg taaaaaagaa aaggtatgat cattgccaca caggttgttg caagcgcaag
      361 cagcactgtt ttttttcttc ctgcttttcc cataagaatt ccggtgggaa tggagaagat
      421 ggcaaaccag aggaatacca tcattggcag tgtccctgcg atccctgacg aaagattgaa
      481 atcgtttttg acataattag tggcgatacc cactatgtct acaaacccca tgatgaaaaa
      541 tccgaaaaaa gcggcaatgg gtgcaaggac attagatttc tttgatgtgt tcattgtatt
      601 atgcattaaa tcttaccgat gctttgatgg taatacattc attccctttg gagttgccat
      661 aaggacggac agtatacgtt tttattcgag cggggacaac aaaggtctcg gcataatgga
      721 caacgaaagg ttcaaaatca ccgtttaagc tctccacaat caccgaatcg ccttctacca
      781 ggtttagtac gtttaccgaa tcgttggtgt agtgagttac tttttccgtg aatatatgcc
      841 tcctggtttc gataaactca tttttatgta agcctgtttt ttcttcgcgt accgtaccgg
      901 tatcgttcag gattttcact ttattcacca gttcacggta ggtaaatccg gtatctcttc
      961 cccacacgat aacctttttg ccccgatcta cgtttatcgg gcgtggtttt ccgtcgagtc
     1021 ccaaccgttg ccagtcccag agcttaaaag taaaaagatt gggtgttgag ctgatttcga
     1081 gtaccaatgt gtcttttccc gaacaatgta cggtcccgcc gggtataagg aaatgatcgt
     1141 gtttctttac gggaacttta ttcacgtatt tatccacatc aaacacaaag aaaggatctt
     1201 tctgagcttt atttagatcg gccaccattt catcgggatt tatgtcgttt ctaaggccca
     1261 gatacacgta agtatcctct ttggtatcca taatgtaata actttcatcc tgtgtatatg
     1321 ccaagccgaa gttttcccgg gcgtattcgg ccgttgggtg tacctgcaaa ctgaggttgc
     1381 cacctcctat ggtatccaaa aggtcgaacc ggataggaaa ttctttgcca aagcgggctt
     1441 cgacagggtc tccaagcaac tccctgcttc tcgtatacac caggttaacc gaaggcatct
     1501 cgaattcaat gtcgttaatt tttaacaata aactgttttc ttcgggtaca cagtcgaaac
     1561 accaaccaaa attactcacc gaacggtcca gatcacacac ttctttcatc cattgtccac
     1621 cccatggtgc cggatcgaaa aaaggtacta accggaaagg agaacttgcc gccttgtcca
     1681 ttcccttgaa gaaagtctct ttgtcaatta gtttgggcag atcaactttg actgtgtcga
     1741 gccagaaatc aacggtgtca tagattgttt ttttgtgctt gtccagtact ttccagtcgt
     1801 tgaaaaggcc gcgtttgtat tgaagagaaa aagtttcctc tttattgttc acacccaggc
     1861 cgttcacttc ctttcttcgc atccgttgct ggatctccca tcggggcatg tctgcgtaaa
     1921 tgttgatgtc gcctgttgtt acatattgtg ccccatatcc gaagacgatc acagtacccg
     1981 atgcctgctc aatactttcc ctggcaaaac ggagtttctc ttcatcgaaa taatcgctga
     2041 tggaaaggtt ggaatagaag ccaaaaagaa tgtcttcggt taaaaactgc tcggtcaatg
     2101 cgttaatttc attttcggat ttaaagatct ccaatgtgtc gataagccgt gcattattga
     2161 tttcttgcag cttagatttt aataagtcat agtaaacgcc tgtatagcag tcgaccgtca
     2221 gcagtttcgc atgttctacc ttcccgctca cgaaagagga aatgcttttc cacccttcaa
     2281 gtataaagcc tttcccgatt gtaacagcag gacgcttatc gtaattgctt tttcttttcc
     2341 ggttcatatt tatagtatat gttgggtttt tcatcgtatg tatgaacatg cttcgggtgc
     2401 aaaaataatc aatatctctg ctgttgaatc ttattttgtg ttttttatgt ttttttaatg
     2461 gatgaggtct ggcttaaaaa ttggaagaaa aagatctgga ctctctgacg ctttcaatct
     2521 tataactgaa tgaatcggct ctgtaccaac cgatattgta ttctattggc agatcgttta
     2581 tgtcgcttac aaatctttta cggataagga ttggatctcc gacttcaatt tctaattttc
     2641 cagcaatgaa tggattggca ccggatgccc ggatttcttc tcttgatgtt tttacgatga
     2701 tattataatc attttccaat atttcgtata atggccggtt aaagttctcc gtaccatcca
     2761 ttctgatttt aggattaaat tccgaatgaa aatagacaaa agggaaattt tctttgccgc
     2821 gcaatctttc taaacgcagg accttcacct ggttagtgat ttcaaaaaac tgcctggcat
     2881 tttcagtggg agcctgtttg ctgatgtgta gttcgaagtt ttcaattttc actcccaata
     2941 ctttcatttc ctgtgaaaaa ctcagccagt tggtagcttc gctgtataaa ttcttttcgg
     3001 caactcttgt ccctacgccc tttttgcgta tcaacaaccc ttccgtgacc aaggtgttga
     3061 tggattgtct taatgtgttt ctcgatattt ttaattgttc agacaattct acttcgttgg
     3121 gcagcatttt cccttctttg tactcttcct tcgttatcaa ggcgcgaaga agcatttctg
     3181 cttgtttgtg tagagggaca gggctgttgc ggtctatttt taatttcatt ttttttgtgc
     3241 aaagtaaaaa atttaagtct cgtcatagat tgatcactgt ctcgtatggc taaataagat
     3301 ctgtattgag gttcttttat tctacttccc gttgattcct gaagtcataa aatgtttttc
     3361 tcatcaggtc ggcgagcgat aattgataat tgatataggc cctcaggtgg gaagtatatg
     3421 cgttgttcaa cctgtttctc tctaaagcca agccttggct gtcgatatct ccgtcagaaa
     3481 aacgctgccg tgttatctcg aaactctttt cagcgactaa aacattcttt tccaggactt
     3541 gtagcctttt caagttgctg tttatgctgg cgatgaggtt tttgacttca gtttctattt
     3601 cccgttcaac ttcttctttg cggtaattgt actgttttag cctcgattcc gctgccctta
     3661 ccaaggcccg gttttccccc cagtccaaaa tgggtatggt gacggtcagt ccgattccgt
     3721 agcttgagtt tctgtcaaca agattgttgt acgagttttt gatcgattgc ataaaactga
     3781 tgtttgcccg gtgatcaata ccggcccttt cgaagtacgc atccacactt ccttttatca
     3841 gtccgttggc tttttgttgc ttaatgttaa gcttttgcaa ctcgatttga atttcctgtt
     3901 cccggacttc caaacggttt ttcagggcta actcaactgc ttttgccgga tcgataagga
     3961 cgatttgata gttcagttgg ttattcaagg ctatgctgtc attcaggtct aatcccaata
     4021 attccttgaa tgaatttctt gctgatactt catttaagac ggcaatatcg tgattattct
     4081 gagcttcggc caaatccacc tccatttgaa gtgcatctac ttcgcggatg agtccggcgg
     4141 caaatttatt ttttgatatt tcgtgagctt ccatctgtcg ctccaggtcg agagacgcaa
     4201 tttcggtgct ccgctgcaac gacagtaaat tgtaatatga actgcttacc tgataaataa
     4261 ggttcaattc ttctcttttg aactgtttgc tggagcgctc ataatccagt tcggcccttt
     4321 tcagggtaga tttaatgctg ttgtaggcat aaaacgaatt gagcggttgg ttaaatccaa
     4381 tccgggaacg caggcgcgat gaacggaaat tgtttttaag gtcttcgctg ctcgacagca
     4441 tattgttgat gaaaatattg ccatcggtag ggagtggttg attgatggtc agcactccgg
     4501 agtatcccag ttcttttacc ggatagtaat aagtaattcc cgtagtatct ttccaactct
     4561 cgatggtttc gttgtactgc ggcgcgccga gggtcatatc gatatgtgtt ttcaaccggc
     4621 ttgttgccga tttcaggttg aattcggcaa ttttgaggtc ttcgcttaac ctcaacatgc
     4681 tgtagctctt tttcttggct atttctatac tctcttccag ggtaagcgtg taagccgact
     4741 ggctttggag caataaacaa taaatgaata taaatgaacc tgctaaaaga agttttttca
     4801 tattatttcg tcattttgaa atgtattcaa agagggtagg gaagtccttt tttggttagg
     4861 cttgtcggaa tgaatcatgc cgtctttcag gtaaatgttc cgatgagcgt aactggcgat
     4921 ttcttgctcg tgagtgatta aaataattgt attcccttcg gcatgcagtt cctcaaataa
     4981 ccgcataatt tctattcctg tttttgaatc gagggcaccg gtcggctcat cggccagcaa
     5041 aatacccgga gtagtcacta acgccctggc aatggccaca cgttgtcttt ggcctccgct
     5101 tagctcagca ggtttatgag ttacccgatc cgacagcccg accctgtcca acgccctcaa
     5161 cgctctttct ttgcgctccg cactcggcgt tcctgcgtaa atcaggggga gttccacgtt
     5221 ttgaatggca tttagtcgtg gcaataggtt gaaattctga aagataaagc ctatttcctt
     5281 gtttcgcatg atcgataggg catcgtcgtc caatgtgctt acatcaacat cgttaaagta
     5341 ataatgaccg ctcgaggccg tatccaggca tcccaggata ttcattaacg ttgactttcc
     5401 cgaacccgac ggtcccataa tagcaacgta ttcgtttttc tcgatgttga ggtttatgcc
     5461 gtgaataacc ggtatttcgg catctcccaa caagtaattc ttttttaagt cttttatttc
     5521 gatcagcatt tttttgactt gtttttattc gtatcgaatg gaatctaccg gttttaaatt
     5581 ggcagcgttc tttgccggta aataaccgaa gctcactccc acgattaccg aaaaagagaa
     5641 ggctataaag acagagtagg gctttataaa agtgtttata tcggtgaaca gcgaaactat
     5701 gagcgacagg acaatgccgg tgaggacacc gatgatacca cctgtgatac ttattgccac
     5761 cgattcggtg acgaactggc tcattatatc tattttacgg gcaccgaccg agcggcggat
     5821 accaatttcc cgtgtccgtt ccatcacggt tgccaacatg atattcataa ttccaatacc
     5881 gcccaccaac agtgatatgg cagcaatagc gcctaacaga aaattataga tttggcgttc
     5941 tttttcctct tgttttagta attcgtaagg aattacaatg ctgtagtcgt cgttattgaa
     6001 atgatggcgg cgcagaattt cgttaataat tttggaggct tctaccagtt tatccgaatt
     6061 ttttagttga acggttatct gctgaatttc actggaaagt gcattctcgc gagtgaaccg
     6121 gttcagaaat aaggagaggg gcacaaacac atccgtattc aggtcacgtg cagccaattc
     6181 gccaacggtt tccgtaaaaa ggctcttcga ttccagtacg ccaataactt ccagccactg
     6241 atcgtcaatt tttaccagtt tacctatcgg atcctctcct ttaaaaaaac cggctgccac
     6301 gcctgcaccc agcacgcaga ctttcaggcg ttcgttataa tgtttgtcgt tgatgaaata
     6361 tccgttccgt accttgtaat tcatcatggc ctgaaagtcg ggggtgatgc cgataatggt
     6421 ggactttacc gatttatcgt tgtatcttac gtctgtgctt ttttcagcct gtgaggcaat
     6481 tctttctact ccgggtacga tttcttgaat cacttgagca tcctggatgg aaagcccttg
     6541 cgagaacttc gcccttactt cttccaactc ttcatcggac agtgtttttt cgcggatgat
     6601 gatgttgttt acacctagat cctggtattt ggcaatggct tcacgcttgg ccccttctcc
     6661 gattgataac attgtgataa cggatgccac accgaagatg attcccaaca tagtaaggaa
     6721 agaacggaac ttatggtcca ctagtccttg aagcgccaaa cgaatatttt caccgtactg
     6781 cataatttac tttataaaaa ttcacaactt actgtcatcc cgggttttaa tcgttcatcg
     6841 ggttttatta tctttacttc cacatcgaat actttctgcc gggatttttg gtctttcagc
     6901 ctgcaaagtt tgccgatata gacaatctcc ccttcaaaaa caactttagg catggcatcc
     6961 agcctcaccg caactttctg gttaagtgct actttcaaaa agtcggtttc gttcacctgc
     7021 gtattcactt tcataaattc cagttccgga actatggcga ggttattgcc ggcatatatg
     7081 ttgtctccga tcttgtaaat gttcctgttg gaattttgcc agtttgtcgc aatttgaaac
     7141 acccctgata cgggtgcgcg taaggtcagc aaaggtatga ggttctttgc attttcaacc
     7201 tcttttttta tttgttcaac cctgatttcc tgtattttaa gctcgctggt attgatagct
     7261 ttgttcagtt cgtagatttt tttctctttt tcgaggttga tgacagcttg ttcgtattcg
     7321 agctgtttta tttttcgcaa tcgctccgat tcgaaccggg aggcctccca ttcaattttt
     7381 ttcaaatcga atgctgcaga ctcgtttctt attttcgatt cgctttcctg ttttttgttt
     7441 tcttgttcta catagagttt ttccagtacc gccagttgtg tttcgaggtt gctctcgctt
     7501 tccaaaatgt acttgttgat atcagccggg tcgagttgcg ctatggaatc gccttttttt
     7561 acaatctctc cgtggttcaa caaccccaca attttcatct ggtaccattg cctcccatac
     7621 cggctgacaa cgaccgaacg ggaatttact gccgccagtt ccccggtttc cacaataaca
     7681 ctgccgtcgg aagttcttct ttcgctctcc gattctgaac ttcttttgca tgaaaaaaga
     7741 agtgccgcga cgaggacaac agaaaacagg ctatataatt gtgatctttt cattatatct
     7801 ccgcgttttc ggttttcttt tcttccgttt cttttgaaaa aggatttacg agtgctatct
     7861 cttcaccttc ttttaatcca ttgacaacca cgatgtagtc ggtgttgttt atcccggttt
     7921 ctatttctgc cttttcgtag ccagtacctt tctttttgta gacaaaattg ttgtctcctt
     7981 cagtgaacag ggcgtctagc ggaatataga gcacatcttc aactttgtcc attattatgc
     8041 ggcagctgac ggtgagcccg ggaagcaaat tcttatcgga ctcgttgagc aatatctcaa
     8101 cggggaacac ctttattttc gatgactcat ctttgttcac agctagattg gcaatcgaat
     8161 tgaccgttcc ggaaaaaaca ctgtcggaaa atgcatcggg tttaatttct actttaagcc
     8221 ctttgctgat tttggctacg tccacttcgt ttatctttac cgtagctttc agcgaagata
     8281 aatcgggcaa ttgaataatg ggaaacccac tccagcattg gtcgccaatc tgaaatttat
     8341 tgttgctact ccagtttttc gataaaatgg ctatacctga agagggggtt acaaccgata
     8401 gcttttgaag tgtttcgtgc gcttcgtcca gcctcgattg gaattgcatg atggagaggt
     8461 ttttttgctt gatctcttct ttctgaattt ttatacggtt ctcaatttgc tctttagctc
     8521 tttccagcgc aatttctgct ttatcgaggt ttagctggat ctcttttttc ttgatatccg
     8581 attcgtaccc ggcagactcg aagcggattt tcgatatttc gtgcgaaatg cgtgtaactt
     8641 cataagctgc cttcagctct tcgagctccg attgttgctg tgctttcatt ttatcgaact
     8701 cggcccgtgc aatctctaaa ctcgattctg cctccacaat accttttaga acttccgacg
     8761 ggtcaaacac aatcagcgtg tctcctgctt tcacttcctg cccgtccttg accaactcgg
     8821 tgattttaag gttcccgtat cgccacgaaa tcatcggtgc tacaatgttg atggacttaa
     8881 tggcctctat ctctccttct tcatacaagt cgagaaataa agtgccctga gttacttttc
     8941 caacaggtat ttgttgggtt tccttttttt tgcacgctac aaaaagtagc gaacaaataa
     9001 cgatgaggat ccatgttttt gaaaaattct tcatattgct gcaatggctt gaaattattt
     9061 tgtttttcct ctcaccatcg atggcggcgg ttttattaac gcaattactt ctcccggttt
     9121 taatccgtct gatacgatag cttctttgga agaagagagt ccggttgtta ttggacgcat
     9181 ctcgtatttg tttttcttct tcacatacac aactttaagg gagtcttctt cataaatggc
     9241 aatttgtgga accacgattg tgtcgcggat ttgttttatg aatacccggc aatttgctgt
     9301 gaaaccgggt tcagggagtt gcagtgcgct gtctacagat gcttctacct caaaaaactt
     9361 taccttcgaa tcccttttat agggttggcc tacgggcagt tttttggtga tttttccata
     9421 ggcgacgttg ttgggcatag catcgaaact gaagctaacg gaatcgttct cgtttatctg
     9481 gcgaaagtcc acttcccccg ccatcatttt taccttcatt tccgaaactt caggcaaggt
     9541 gatcaacggc atgttgttcc atacattgtc gcccaccttc agtttttctc ctgtcatccg
     9601 ggaattagag acaacggcta gtcctttatg cggggagcgt atggttaagc cgtcgagaat
     9661 ggtctttgcg gaggctaacc ggttggttaa ttgttgaata ttaagctcca attttcttac
     9721 ctctgattga ttgatgatct cgagagcttc gagcttcttc tcaaatttat ttttttcgat
     9781 tagcgcccgt tcaagctcta attccttgat tcgtttctgt gtaggagtgg cgaaatgtaa
     9841 ttggagggag tctaattgcg ctatttcggt atcggcttta ttgtttttta cttgtgcctc
     9901 aagcagggcg tactgcatct gtaaatcagc ttttaccttg cttaattcgg ctactgcagt
     9961 ttccaactgc atcgctaagt tatcgtgaag ggttttacgg tcattgtctt cgagaatgca
    10021 cacaacatct ccttcattga catacgttcc gtcatcaata aggaaagtaa taatggcatc
    10081 cacattgcta ggacaggcta ttgttgtgta tcggacaggt tcagcaacgc cgtcgatcat
    10141 cagggtgttt tcaaaattgg acgttagtac agggtatgtt gagatctttt ctttattgct
    10201 tttgttgcat gaacagatta acaataaaaa ggccaacatc cataaaatta catatctttc
    10261 cacgatagtg ttttgtagat catggtcgca aatgtacgat cttctttttt gtttacgaaa
    10321 attaagtaga ttcaattaga aaaatttatg atttgttctg aaaagtttaa aatcgtggaa
    10381 aaactgcgtt acggggccag cctgtgtaac gtccaaccct cttcagtccg gtagtataca
    10441 agcctgtcgt gcagacgacc aggacgtccc tgccagaatt cgatggttgt agggagaagg
    10501 ataaagccac cccaatatga cgggcggaaa atttccaggt cattgaattt tttctcgaaa
    10561 gaggcctgtc gttgttctaa ttcctcacgg ctccccaata ttttgctttg cggtgaggcc
    10621 catgctccga tttgtgcctc gcggggacgt tcattgaaat aaacatcgga gtcaagggtg
    10681 gagagttttt ctacccgtcc ttcaacgcgg atctgacgtt caatggaatg ccagtcgaaa
    10741 acaagtgcgg caaacggatt ttcgagtaac tccctgcctt tccggctaag gtaattggtg
    10801 aaaaacacaa atccgttttt atttatttct tttaacagca ctaccctggc ggagggcttt
    10861 ccacttgcgg tgcatgttgc aatagtcatg gcgtttgctt ccgggacccc tgagtcgata
    10921 gctgcctgta gccactgctg aaattgttcc attggattgg ctttcacttc agtctcattt
    10981 agttcccctg ttgtatattc ttccctgaat tcaaacagtt ttttcatttg tgatcgtttt
    11041 ttctgataaa gcaacccgat aacctttagc tgccggtcca catcgcgagt tgctatgcaa
    11101 atataagcca tgctgcagct atctgacagt gaggtttagt taataaatac taacgatttt
    11161 ttcttgtaat cctattaaat tcaataagac agagtaattt gctatcttcg cacaggattt
    11221 agaaataatg tttcgaatga taaaggttga aaagatacag aagagttttg gtgaccttca
    11281 ggtgttgaag ggaattgatt tggaagtggg taaaggggag atcgtatcga tcgtgggtcc
    11341 cagcggtgca ggaaaaacca cgctgttgat gattatgggc acacttgaaa aggcaaattc
    11401 cggaaaggta atgattgaca acaacgactt gagtaaactc agcgagaaaa aacttgcgga
    11461 ttttcgtaac aagaatatcg ggtttgtttt tcagttccat caacttctgc cggaatttac
    11521 ggctttggaa aacgttatga tacccgcact gatcggtaac atgaagcagg gtgcggcgga
    11581 gaaaaaagca aaaaaacttt tggctatgat ggggctgagt gaacgaatgg aacacaagcc
    11641 tgctgaacta tccggaggag aaaagcagcg tgtggcagtt gcccgcgcac tgataaacga
    11701 tcccgtagtc gtttttgccg atgagccttc ggga
//
LOCUS       DAOSDR010000037        11664 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrimonas sp. isolate AMR_MDS_1826
            NODE_2129_length_11664_cov_4.193126, whole genome shotgun sequence.
ACCESSION   DAOSDR010000037 DAOSDR010000000
VERSION     DAOSDR010000037.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863355
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrimonas sp. (wastewater metagenome)
  ORGANISM  Petrimonas sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Dysgonomonadaceae; Petrimonas.
REFERENCE   1  (bases 1 to 11664)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11664)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:10:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,720
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 35
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11664
                     /organism="Petrimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2129_length_11664_cov_4.193126"
                     /isolate="AMR_MDS_1826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2023866"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..3289
                     /locus_tag="PLI30_02575"
     CDS             <1..3289
                     /locus_tag="PLI30_02575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019538595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2723 domain-containing protein"
                     /protein_id="HOI78470.1"
                     /translation="MLLGNLFTQFTNDPGQVAKMVNSMSALLSAFTILFLFWTITHLT
                     RKLVMGEKNDAFSLGQTIAVIGSGLVGALVYTFSDTFWFSAVEGEVYAFSSMLTALVF
                     WLILKWEENAEKPDSDKWIVLIAYIMGLSIGVHLLNLLCIPAIVMVYYYKKTENPTWK
                     GGLFSLFLSFGLILILMYGIIPGFTKVGGWFELFFVNTLGMSYNTGVAVYLILLVASI
                     VWALFESISDRGDIKRARIAFLLSIGLSGILFIGGSIWLWLVLIATAIYFVFSKNKLN
                     IKFLNLSMSSLLVILIGFSAYAIIPIRSSANTPLDLNSPEDVFSLGSYLNREQYGQTP
                     IIYGTTYASQIVRDNQGRAEISKEKKSYSRVLQTAENQKDRYVESKIPTYKYTNTMLF
                     PRMHTHPSEPGYGNHIQGYEIWGGITDRSKKPTLFDNLKFLFNYQINFMYWRYFMWNF
                     SGRQNDIQGDGGITKGNWITGIKFIDGPILGLGPQDNIAPEVADSKGHNKYYLLPFLL
                     GVIGIIYQLNLKRKGKQSFSIVFLLFFMTGLAIVLYLNQTPYEPRERDYAYAGSFYAY
                     AIWVGIGVAGISRYLRNYIKNTTLSATLVSAACLLVPLQMAGQNWDDHDRSGRTLARD
                     TGMNYLSSVEPDAILFTNGDNDTYPLWYAQETEGFRTDVRVTNLSFLQTEWYVDQMLR
                     QAYESAPLPIKWDREKYWGDAASAAFVVTKNEIQNVLKQNNIPSISYGQYYDVNAYRD
                     SIPLKEIMENLRTGQYKPANPFSTGDTQIIPSNRLYLNVDSATTDWKAFNSRPADKMF
                     LNLGEKSALYRQEMMIMEMLTNINDDNWKRPIYYATTVDRNLYMNLQNSNFSLTGLAY
                     QIVPGIPQSGGVNTEKAYDNLMNKFRWGGLEENPDIYLDETGRRMISTFRLYFNQLIE
                     ALTEEGKNDKAIAALDKVTTVMPGKAVAYGNDGIMFARAYYRLGETEKAQRLMDEIEE
                     RLQKNLSWYDRLTPRQISNTMVDIYYNVNSLLLIASVYQEFDKQKYKTYTDDLLQRAQ
                     TYYMQGAGYVGDVILKDITDSSIRGYYRSENDTAQRASEEATMQQALKLMQQFSPRLL
                     EQYNKQQ"
     gene            3312..3932
                     /locus_tag="PLI30_02580"
     CDS             3312..3932
                     /locus_tag="PLI30_02580"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009318360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HOI78471.1"
                     /translation="MLIEQPPHLYRMLFPNAVWRIEDPVRKTVYLTFDDGPIPEVTPW
                     VLDLLDRYGVQATFFCVGDNVRKHPEVYRQVIEKGHRTGNHTFHHLQGWKHSLGKFLD
                     NAGQARELIDSSLFRPPHGHLGMNQCRKLQDAGYRIIMWDVVTRDYSRFMSPGQVLQN
                     VKRYTRNGSILVFHDSLKAEKNLRYALPRAIEWLLKEDYTFELVPL"
     gene            3988..5295
                     /locus_tag="PLI30_02585"
     CDS             3988..5295
                     /locus_tag="PLI30_02585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019539490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOI78472.1"
                     /translation="MERRKFIRNSASSVLAGLLAPSFLQSSSLFANRTGANDKINVGL
                     IGCRSMGWANLSDFLIHPEIDCIALCDVDQHVLSGRAADLVKLRNKKPELYGDYRKML
                     ERKEIDVIIIGTPDHWHCLQMTDACAAGKDVYVEEPIANSIAECDAMVAAAKRHNRVV
                     QVGQQQRSGNHWHEMKRYIDSGKLGKIAQVNVWANFNYAAILNPVPDSPVPAGVDFNF
                     WLGPAPVRTFNEKRFHGSWRMFWDYGGGLMTDWGVHLLDMALWGMNVTEMPNRVIGSG
                     GNFAYPGNYAETFDTLSVIYDFNDFTIQWNNIAGTETGPYGRNYGLAFKGTNGTLVIN
                     REGWEVIPEKNKAEPVKGEPDFQDHKKHVARFIECVKKRDFQTACTIENGSLCAKYAH
                     LGNISARTGATLMYDDNKKSFNNDKADRLIKPDYREPWKFPKA"
     gene            5340..6095
                     /locus_tag="PLI30_02590"
     CDS             5340..6095
                     /locus_tag="PLI30_02590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006797922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1080 domain-containing protein"
                     /protein_id="HOI78473.1"
                     /translation="MKRQNLIILTGLIVLLPLNAQEKKYPERAPMTPAMTEYWQPQPP
                     VVTPDPVPSDAIVLFDGKNLSQWENSKGAPAEWTVHNGVFTVKKGTGDIQTKQKFSDF
                     QLHLEWRIPEGIKGESQARGNSGLFLQGIYEIQILDNYRNETYVNGQTGSIYKQTPPL
                     VNAMRKPGEWNTYDIIYTAPTFKKDGTYRTPPTVTLLQNGILLQNNTIIRGTTPYVGF
                     PEVKEHGPGPIKLQDHGDPSQPISFRNIWIREL"
     gene            complement(6082..6285)
                     /locus_tag="PLI30_02595"
     CDS             complement(6082..6285)
                     /locus_tag="PLI30_02595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI78474.1"
                     /translation="MHLGDIEKIGGEIPLPSDKIAEIYRKTRKGLQAEINLPGDVNGS
                     FVWKGKSYALKGGSNKLDISTAP"
     gene            6587..7933
                     /locus_tag="PLI30_02600"
     CDS             6587..7933
                     /locus_tag="PLI30_02600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019541155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na(+)-translocating NADH-quinone reductase
                     subunit A"
                     /protein_id="HOI78475.1"
                     /translation="MANRIKIKKGLQIPLLGKPEETLLGSITSEYVQVCPEDFQGITP
                     KLKVKVGDTVKAGQALFFSKMHPDMMIASPVSGTVTAINRGEKRRILNVTVKADKENT
                     YVEYGKSEIGTLPPEAIKKRLLDAGIWFVIKQRPYDVVADPGKEPRDIFVTGFDTAPL
                     APSYDFILKGQEADLQTGLNALARLTKGKVFLSISPATKNEGLRKAANVTITEFEGPH
                     PAGNTGTHINYLAPVNRGEVVWTLNALDVLFIGRLFNKGVVDLTRTVALTGSEVKQTG
                     YYNMLVGTELGKLFADNVNGKGNLRYISGNVLTGTRIEAEGVLRATHSQLTVIPEGDD
                     VHEAFGWASLSPKRYSAGATYLKLKKAYRLDARLLGGPRAIIVSNEYDKVFPLDIFPE
                     QLIKSTLAFNIDKMEQLGIYEVAPEDFALCEFVDTSKLELQRIVRTGLDMLRKEME"
     gene            8002..9207
                     /locus_tag="PLI30_02605"
     CDS             8002..9207
                     /locus_tag="PLI30_02605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007656844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit B"
                     /protein_id="HOI78476.1"
                     /translation="MSLRKFLDKIKPNFEEGGKFHWLNSTYDAFETFLYVPNKTSKSG
                     VHIHDARDSKRTMVIVILALIPALLMGMYNVGYQHYLAINVQAGFVGTFLYGLLAMLP
                     QIVVSYVVGLGIEFAMAQMKKEEVAEGFLVSGLLIPMILPVDTPLWMIAVATAFSVIF
                     AKEVFGGTGYNIFNVALIARAFLFFAYPSKMSGDQVWVRTADTFGLGGGNVVDGFSGA
                     TPLGQLAVSDASRFGDFSFLGITGNPLSLWDMFIGLIPGSVGEVSTLAILLGAILLII
                     TGVGSWKTMTSVFLGGLFTVALLNLLAQNAAMEMPPVYHFLLGGFAFGAVFMATDPVT
                     SARTEKGKWIYGFLIGVVAILIRIFNPGYPEGMMLAILFMNAFAPLIDFYVVDANIKR
                     RLKRAAVKK"
     gene            9248..9943
                     /gene="nqrC"
                     /locus_tag="PLI30_02610"
     CDS             9248..9943
                     /gene="nqrC"
                     /locus_tag="PLI30_02610"
                     /EC_number="7.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019541157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit C"
                     /protein_id="HOI78477.1"
                     /translation="MNRENSTYTIIYASVMVIIVAIGLAFTHQVLSDRQTANVNIDKM
                     QQILRSLRVDASPAEADGIYRALVKSAYLITPEGVKIDGTEGIEPTDPAFSGEGEGLA
                     VYEAEVEGSKKYIIPMNGTGLWGPIWGYLSVEGNGSTVYGSEFGHAGETPGLGAEIVY
                     PAFRNQFNGKELIKNGEFKSIAVVKPGQTNSERDYVDGISGGTITSKGVDAMLLESVG
                     EYKNFLLQLNDGK"
     gene            9973..10605
                     /locus_tag="PLI30_02615"
     CDS             9973..10605
                     /locus_tag="PLI30_02615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019541158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit D"
                     /protein_id="HOI78478.1"
                     /translation="MALSAKAKQALLGPLNKNNPILVQVLGICSALAVTSKLEPSLVM
                     AIAVTIVTAFGNVIISLLRKTIPNRIRIIVQLVVVAALVTIVSEVLKAFAYDVNKQLS
                     VFVGLIVTNCILMGRLEAFALGNGPWPSFLDGIGNGLGYGWILVTVGFFRELLGSGTL
                     LGYKVIPQAAYSAGYENNGLMMLAPMALILVAVIIWVHRSKNKDLQEGTN"
     gene            10683..11297
                     /gene="nqrE"
                     /locus_tag="PLI30_02620"
     CDS             10683..11297
                     /gene="nqrE"
                     /locus_tag="PLI30_02620"
                     /EC_number="7.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018360187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit E"
                     /protein_id="HOI78479.1"
                     /translation="MDAISLIVRSIFVDNMIFAYFLGMCSYLAVSKNVKTAFGLGIAV
                     TFVLVLTLPINYMLENYVLKAGALSWLGPQLATVDLSVFSLLIFIAVIASFTQLVEMV
                     VEKFSPALYNSLGIFLPLIAVNCAILGGSLFMQQREFANVGMATAYGFGSGIGWMLAI
                     VGMAAIREKLEYSHVPKPLKGLGITFIITALMAIGFMSFSGINI"
     gene            11374..>11664
                     /locus_tag="PLI30_02625"
     CDS             11374..>11664
                     /locus_tag="PLI30_02625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012457259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HOI78480.1"
                     /translation="MSSGGITILTSVVVFLLIILILVIVILVAKAKLMPSGNVKITVN
                     KEKNLEVPMGSTLLNTLQSQNIFISSACGGGGTCGQCRCQVLNGGGEILPTET"
BASE COUNT         3215 a         2894 c         2835 g         2720 t
ORIGIN      
        1 tatgcttctc gggaacctgt tcactcaatt caccaacgat cccggacagg tggcaaaaat
       61 ggttaacagc atgtcggccc ttctgagcgc tttcaccatc cttttcctgt tttggaccat
      121 cacacacctg acccgcaaac tggtgatggg ggagaaaaac gatgcgttca gccttgggca
      181 aactatcgcc gtgatcggca gcggcctggt aggtgcgctg gtctacacat tttccgacac
      241 gttctggttc tcagctgtag agggtgaggt atatgccttt tcatcgatgc tcaccgcact
      301 ggttttctgg ctgatactga aatgggaaga aaatgcggaa aaacccgatt ccgacaaatg
      361 gatcgtactg attgcctaca tcatgggact ctctataggc gttcacctgc ttaacctgtt
      421 gtgtatcccc gctattgtga tggtttacta ctacaaaaag accgaaaacc ccacctggaa
      481 aggcggatta ttttcgttgt tcctttcttt cgggttgatc cttattttga tgtacggaat
      541 tattccggga tttaccaaag tgggtggatg gttcgaactt ttctttgtga acacactggg
      601 catgtcctac aacacgggcg ttgccgttta cctgatcctg ctggtggcaa gtatcgtctg
      661 ggccctgttc gagagcatct ccgacagggg cgatataaaa agggctagaa ttgccttttt
      721 gttgagcatc ggcctttccg gtattttatt tattggaggc agtatctggt tgtggctcgt
      781 tcttattgcc acagccatct atttcgtttt ctccaaaaac aagctaaaca tcaagtttct
      841 gaacctttcc atgtcgagcc tgctggttat ccttatcgga ttttcagcat atgccatcat
      901 tccgatccgg tcatcggcca acaccccgct cgacctgaac tcaccggagg atgtgttctc
      961 cctcggcagt tacctcaacc gagaacagta cggacaaacg cccatcattt acgggactac
     1021 ctacgcttcg caaattgtca gggacaatca ggggcgcgct gaaatttcaa aagagaaaaa
     1081 atcgtacagc agggtattgc aaacggccga aaaccagaaa gaccggtacg tagaatccaa
     1141 gatacccaca tacaagtaca ccaacaccat gctctttccg cgcatgcata cccacccgtc
     1201 ggaaccggga tacggcaacc acattcaagg gtatgagatt tggggcggca ttaccgacag
     1261 aagtaaaaaa cccacactgt tcgacaacct gaaattcctg tttaactacc agattaattt
     1321 tatgtactgg cgttatttca tgtggaattt ctccggcagg caaaacgata tccagggaga
     1381 tggcggaatt accaaaggga actggatcac cggaatcaaa tttatagacg ggcctatcct
     1441 tggcctgggg ccgcaggaca acattgcacc cgaggtcgcc gacagcaaag ggcacaataa
     1501 atactacctg ttgcctttcc tgttgggggt tatcggcata atttaccagc tgaacctgaa
     1561 acggaaaggg aagcaaagtt tctccatcgt ctttctgttg ttctttatga caggattagc
     1621 catcgtgctg tacctcaacc aaacgcctta cgaaccccgc gaacgggatt acgcttatgc
     1681 cggatcattc tatgcctacg ccatctgggt gggtataggc gttgccggaa tcagtcgata
     1741 tctccgcaac tacatcaaga ataccacgct gtcagcaacg ctggtcagcg cggcctgcct
     1801 gctcgtcccc cttcaaatgg cgggacagaa ctgggacgac cacgaccgtt ccgggcgaac
     1861 cctggcccgc gataccggca tgaactacct cagcagcgta gaaccggatg ccatcctctt
     1921 taccaatggc gacaacgaca cctacccgct ttggtacgct caggagacag aaggcttccg
     1981 caccgatgtc cgcgtcacca acttaagctt cctgcaaacc gaatggtacg tggatcaaat
     2041 gctccgtcaa gcatacgaat ccgcaccgct acccatcaaa tgggacaggg agaaatactg
     2101 gggcgatgcg gcaagtgcag ctttcgtagt tacgaaaaac gaaatccaga acgtacttaa
     2161 acagaacaac atacccagca tttcctacgg acagtattac gacgtaaacg cctaccggga
     2221 cagcattccc ctcaaggaaa taatggaaaa cctgcgtacg ggccaataca aaccggcaaa
     2281 tccgttcagc acgggagata ctcagattat cccttccaac aggctctact taaacgttga
     2341 ttcggcaaca acggattgga aggcattcaa ctcaaggccg gcagacaaaa tgttcctcaa
     2401 cctgggtgaa aaatcggctc tttaccgaca ggaaatgatg attatggaaa tgctgaccaa
     2461 tatcaatgac gataattgga aacgcccgat ctactatgcc accacggtcg accggaacct
     2521 gtatatgaac ctgcaaaaca gcaatttctc cctgacggga ttagcctatc agattgttcc
     2581 gggaatcccg caaagcggtg gcgtgaacac tgaaaaggct tacgacaacc tgatgaacaa
     2641 attccgctgg ggcggactgg aagaaaatcc ggacatctac ctcgacgaaa ccggccgacg
     2701 aatgatttcc actttcaggc tctacttcaa tcaactgatc gaagccctga ccgaagaagg
     2761 caaaaacgac aaagccattg ccgcgctgga taaagtaaca acggtcatgc cgggaaaagc
     2821 ggtagcctat ggcaacgacg gcattatgtt cgcccgggct tattaccggt taggcgaaac
     2881 ggaaaaagcg cagcggctga tggacgaaat cgaggaaagg ctacagaaga acctgagttg
     2941 gtatgaccgg ctgaccccaa gacaaataag caatacgatg gttgatatct actacaacgt
     3001 caactcgctg ctgctgatag catcggttta ccaggagttc gacaagcaga agtacaaaac
     3061 ctatacggac gatttactgc aacgggccca gacgtattac atgcaagggg cgggatatgt
     3121 aggcgatgtg attttaaaag acataacaga cagttccatt cgcggatatt accgttcgga
     3181 aaacgatact gctcaacggg cttcggaaga ggcaaccatg caacaggctt taaagctgat
     3241 gcagcagttc agcccccggc tactggagca atacaataaa caacagtaaa cagttatccg
     3301 aagcatcttc tatgttgatt gagcaacctc cccatttata caggatgctg tttccgaatg
     3361 cagtctggag aattgaagac cctgtacgga aaacagttta tctcacgttc gacgacgggc
     3421 ctattcccga agtgacgccc tgggtactcg acttgttgga cagatatggc gttcaggcca
     3481 cttttttctg cgtgggagac aatgtacgca aacaccccga ggtataccgg caagtaattg
     3541 aaaaaggaca tcgtacggga aatcacacct tccaccattt gcaagggtgg aagcacagct
     3601 taggaaaatt ccttgacaac gccggtcagg caagagaact gatcgactca tcgctgtttc
     3661 gtcctccgca cggccacctc gggatgaatc agtgccggaa actgcaggac gcgggctacc
     3721 ggatcattat gtgggacgtt gttacacgtg attacagcag gttcatgtcg cccggacagg
     3781 ttttgcaaaa cgtaaagcgt tatacccgga acggatcgat ccttgttttt cacgactcgc
     3841 taaaagctga aaaaaacctg cgatacgcgc tacccagggc tattgagtgg ttgttgaagg
     3901 aggactatac atttgaattg gtccctctgt agaaaggcaa aaaatttact gaaaacatac
     3961 ctgaaacctg cttaatcttt tatcacaatg gaaagaagaa aatttatacg taattctgcc
     4021 tcgtcagtcc tggccgggct gcttgccccg tcgtttttgc aaagcagcag cttgtttgct
     4081 aaccgtacag gggcaaacga caagatcaat gtcggactga tcggatgccg cagcatggga
     4141 tgggccaacc tgtccgactt cctgatccat cccgaaatag attgcatcgc cctgtgtgac
     4201 gtcgatcagc atgtactcag cggaagggct gcagatctcg ttaagctcag gaacaaaaag
     4261 cctgaattgt atggcgatta ccggaaaatg ctggagagaa aggagatcga tgtgatcatc
     4321 attggaacgc ccgaccactg gcactgcctg cagatgaccg acgcctgcgc agccgggaaa
     4381 gacgtttatg tggaagagcc catagccaat tccattgccg agtgcgatgc aatggttgcc
     4441 gcggcaaaga gacacaaccg ggtagtacaa gtgggacagc aacaacgaag cggcaatcac
     4501 tggcatgaaa tgaaacgcta tatcgacagc ggcaagctgg gcaagatcgc ccaggtaaat
     4561 gtatgggcta acttcaatta cgcggccatc ctcaacccgg tccccgacag ccctgtccct
     4621 gcgggcgtcg attttaattt ctggctgggc cctgcacccg tgaggacatt caatgagaaa
     4681 cgttttcacg gttcgtggcg catgttctgg gattacggcg gagggctaat gaccgactgg
     4741 ggagtacacc tgctcgacat ggctctttgg gggatgaacg taacggagat gcccaaccgg
     4801 gtcataggct cgggaggaaa tttcgcttat cccgggaact acgctgaaac gttcgatacc
     4861 ctttccgtaa tatacgactt caatgacttc accatccagt ggaacaacat tgcaggcacg
     4921 gagaccgggc cttacgggag aaactacggc cttgccttta aaggcacaaa cggaacattg
     4981 gtcatcaacc gcgaaggatg ggaagttatt ccggagaaga acaaagctga accggtaaaa
     5041 ggcgaacccg attttcagga ccacaaaaag cacgtggcaa ggtttatcga atgtgtgaaa
     5101 aaacgcgatt ttcagacagc ctgtaccatc gaaaacggta gcttgtgtgc taaatatgct
     5161 catctgggca atatttcggc acgtacagga gcgacgctta tgtatgatga caataaaaaa
     5221 tcattcaaca acgataaggc cgacaggtta atcaaacccg attaccgcga gccctggaaa
     5281 tttccaaaag cataacgcat aaacaaaagg tcaactcaca acattaaaga aaagacaaca
     5341 tgaaacggca aaatctgatc attttgacag gattaatcgt cctgcttccg ctaaacgcac
     5401 aggaaaaaaa atatcccgaa cgcgcaccca tgacaccggc catgaccgaa tactggcaac
     5461 cccaacctcc ggtggttacc cccgatcccg tcccatccga tgccattgtc ctgttcgacg
     5521 gcaaaaatct ttcccaatgg gagaattcaa aaggtgcccc ggcggaatgg acggtacaca
     5581 acggcgtttt caccgtaaaa aaaggtaccg gggatattca gacgaaacag aaattcagcg
     5641 actttcaact gcacctggag tggcgcatac ccgaaggcat taaaggtgaa agccaggcac
     5701 ggggcaacag cggtcttttt ctgcaaggta tctacgagat tcagatcctc gacaactacc
     5761 ggaatgaaac gtacgtgaac ggtcagaccg gaagtattta taaacaaaca ccgcccttgg
     5821 taaacgcgat gcgaaaaccc ggcgagtgga atacctacga catcatttac accgcaccca
     5881 cattcaaaaa agacgggact taccggaccc ctcctaccgt aaccctttta caaaacggta
     5941 tcctgctgca aaacaatacg atcatccggg gtaccacccc ttatgtagga tttccggaag
     6001 tgaaagaaca cggccccggg cccatcaagc tgcaggatca cggcgacccc agccagccca
     6061 ttagtttccg gaatatctgg atcagggagc tgtagagata tccagcttgt tggatcctcc
     6121 cttcaacgcg tagctcttcc ctttccagac aaaagatccg tttacgtccc caggcaaatt
     6181 gatttcagcc tgtaatcctt ttcgcgtttt ccggtaaatc tctgcaattt tatcggaagg
     6241 gaggggtatt tctccgccga tcttttcaat atctcccaga tgcatgttat cttaactttc
     6301 ttgaaataag gatcgtgctc tctcccccag gacaatgcgg aaaaacttaa cattgggatg
     6361 gagcccgggc attacgaccg gaaggctgtg attcagcaaa actgggaatt aacttatttt
     6421 cagaataaag atttttttca ctattcttcc ttttttgaat ataaaacagt tatctttgca
     6481 ccttaaacgg aaaatagcta tccctcctca cagcttattt taaatagaac tctgattgat
     6541 agttgtttat cctgagaaaa ccaacagtta tttttatcaa ttacatatgg ctaatcgaat
     6601 taaaatcaag aaaggcttgc agattccttt gttagggaaa ccagaagaaa cacttctcgg
     6661 cagtataaca agtgaatatg tacaggtgtg cccggaagat tttcagggaa tcacgcccaa
     6721 actaaaagtt aaggtgggtg ataccgtgaa agccggacaa gcgttatttt tctctaaaat
     6781 gcacccggac atgatgattg catctcccgt cagcggaaca gtcacagcca tcaaccgcgg
     6841 agaaaaaaga cgtatactga acgttactgt caaagccgac aaagaaaaca cttacgtaga
     6901 gtacggcaaa agtgagatcg gtacgttgcc tcccgaagca atcaaaaaga gactgctcga
     6961 cgccggcatc tggtttgtca tcaaacaacg cccttacgac gtcgttgccg atcccggaaa
     7021 agaaccgcgc gacattttcg ttaccggatt cgacacggct ccgctggccc cctcatacga
     7081 tttcatcctg aaaggccagg aggccgacct gcaaaccggg ttgaacgcat tagccagact
     7141 caccaaagga aaggtgttcc tctccataag ccccgctacg aaaaacgaag ggctgaggaa
     7201 agccgcaaac gtaacgataa ctgagtttga agggccacac cccgccggaa acaccggcac
     7261 tcacatcaac tacctggcac ccgtgaacag gggcgaagta gtctggacat taaatgccct
     7321 ggatgtgttg ttcatcggaa ggcttttcaa caaaggcgtg gtagacctta cccgcaccgt
     7381 ggcacttacc ggatcggaag tcaaacaaac cggctactac aacatgcttg tcggcacgga
     7441 gctgggcaag ctctttgctg acaatgtcaa cggaaaaggc aacctgaggt acatcagcgg
     7501 aaacgtgctc accggaaccc ggatagaggc agaaggcgtg cttcgcgcca cccattcgca
     7561 gctgaccgta ataccggaag gtgacgatgt tcacgaagct ttcggatggg catccctgtc
     7621 gcccaaacgc tacagcgccg gcgccaccta cctgaaactg aaaaaggcct atcgcctgga
     7681 tgcacgcctg ctgggcggcc ccagagccat catcgtctcc aacgaatacg acaaggtatt
     7741 ccccctggat attttccccg aacagctgat aaaatccacc ctggcattca acatcgataa
     7801 gatggaacag ctcgggatct acgaagtggc gcccgaggat tttgcacttt gcgagtttgt
     7861 ggacacctcc aagctggagt tgcagcgtat tgtacgcaca ggattggata tgctgagaaa
     7921 agagatggaa taaccggtac ggccctccgc tcaacgcatt gacaaatata gaataacaat
     7981 aaattaaaac agataaaata aatgtcgtta agaaaatttc tcgataaaat aaaaccgaat
     8041 ttcgaagaag gaggtaaatt tcattggttg aattctacat acgatgcctt tgaaaccttc
     8101 ctgtacgtac ccaacaagac atcgaaatcg ggagtacata tccacgatgc cagagactca
     8161 aaacgtacga tggttatcgt gatactcgca cttattccgg cattgctcat ggggatgtat
     8221 aatgtgggtt accagcatta tctggccata aacgttcagg caggctttgt gggaactttc
     8281 ctctacggac ttttagccat gcttccacag atcgttgttt cctacgtggt gggcctgggc
     8341 atcgaatttg ccatggctca gatgaagaag gaagaagtgg ccgaaggttt tttggtctcg
     8401 ggattgctta tcccgatgat tttacccgtg gacaccccgc tctggatgat tgccgtagcc
     8461 actgcgttct ccgtcatttt tgcgaaggaa gtttttggcg gaaccggata caatattttt
     8521 aacgtggcgt tgatcgcccg cgcattcttg ttctttgctt atccctctaa aatgtcgggc
     8581 gatcaggtat gggtacgcac tgccgacacg ttcggcctgg gtggaggaaa tgttgtagac
     8641 ggcttttccg gtgcaacacc gctcggacaa cttgccgtat cggacgcttc acgtttcgga
     8701 gacttctctt tcctgggaat cacaggtaat cctttgtcgt tgtgggatat gtttatcgga
     8761 ttaattcccg ggtcggtcgg tgaggtatcc accctggcta tactgctggg agccatcctg
     8821 ctgatcatca cgggcgtagg aagctggaaa acgatgactt ccgtcttcct gggagggctg
     8881 ttcaccgttg ccctgcttaa tttgctcgca caaaatgccg ctatggaaat gccgccggtg
     8941 tatcacttcc tgcttggcgg tttcgctttc ggggctgtat tcatggcaac cgatccggtg
     9001 acatcggcac gcacggaaaa ggggaaatgg atatatggtt ttctgattgg tgtggtggcc
     9061 atcctgatcc gtatcttcaa cccggggtat cccgaaggca tgatgcttgc catcctgttt
     9121 atgaacgctt tcgctccgct gatcgatttc tatgtggtgg atgccaacat caaacgccgc
     9181 ttaaaacgag ctgccgtgaa aaaataggga ttcgcgtttc tgaataacta ctgaatacaa
     9241 ttgaactatg aacagagaaa atagcacata tacaataata tacgcatcgg taatggtgat
     9301 tatcgttgcc ataggtctgg ccttcaccca ccaggtgttg agcgacagac aaactgcaaa
     9361 cgtgaatatc gacaagatgc agcagatcct ccggtcgctg cgcgttgatg cctccccggc
     9421 cgaagccgac ggcatctaca gagccctggt taaatcggcc tacctcatta ctcccgaagg
     9481 agtaaagatc gacggcacgg aaggaattga gcccaccgat cccgcttttt cgggcgaagg
     9541 ggaagggctt gctgtctatg aagcggaagt ggaaggttcc aaaaaataca ttatccccat
     9601 gaacggcacc ggcctgtggg ggcctatctg gggatacctc tccgtagaag gcaacggcag
     9661 taccgtttac ggttccgaat ttggacacgc gggtgaaact ccggggctgg gggcagaaat
     9721 agtatacccc gctttcagga atcaattcaa cgggaaagag ctgataaaaa acggcgaatt
     9781 taaatccata gccgtggtta aacccgggca aaccaactcc gaaagggact atgtcgacgg
     9841 aatatcgggt ggcaccatca ccagtaaagg agttgacgcg atgttgctgg aaagcgtggg
     9901 agaatacaag aattttttac tgcaattaaa tgacggaaaa tagatccgca ttcattataa
     9961 aacgataaga atatggcatt atcagcaaaa gcaaaacagg ctctgttagg gcctctcaat
    10021 aaaaacaacc ctatcctggt acaggtgctc ggcatctgtt cggcactggc tgtaacgtcc
    10081 aagctggaac cgtcattggt aatggccatc gccgtaacca ttgtcaccgc tttcggaaac
    10141 gttattatct cgctgttgag aaaaaccatc cccaaccgta tccgtatcat cgttcaactg
    10201 gtggtggtgg ccgcattggt gaccatcgta agcgaagtac tcaaagcatt tgcttacgac
    10261 gtgaacaagc agctgtcggt ttttgtggga ctgatcgtta cgaactgtat actgatgggg
    10321 cgcctggaag cgtttgccct gggtaacggg ccgtggcctt ccttcctcga cgggatcggt
    10381 aacggactgg gatacggatg gatactggta acggtgggct tcttccgcga actgctggga
    10441 tccggcacgc tactcggata caaagtcatc ccgcaggcag catacagtgc cggttacgaa
    10501 aacaacggtc ttatgatgct ggcccccatg gcgttgatcc ttgtcgcggt aatcatctgg
    10561 gtacaccggt cgaaaaacaa ggatttacag gaagggacca attagtgcgc tacgcgtgtt
    10621 aaccggataa ctatcgagta tttatcgaaa aattatcgta aaacaaaaat aaaaaaataa
    10681 atatggatgc tattagtcta attgtaagat cgatatttgt agacaatatg atattcgcat
    10741 acttcctggg tatgtgttcc tatcttgctg tatcaaaaaa cgttaagacg gctttcgggc
    10801 tgggcattgc ggtcactttc gtgctcgtgc tcaccctgcc catcaactat atgctcgaaa
    10861 actacgtact taaggcaggt gctttatcgt ggttgggccc gcaacttgca acggtcgacc
    10921 tgagtgtgtt cagcctgctc atttttatcg ccgttattgc atcgtttacg caattggttg
    10981 aaatggtggt tgagaaattc agccccgcac tgtacaactc gctgggtatt tttctaccgt
    11041 tgattgccgt aaactgtgcc atcctcggag ggtcactgtt catgcagcaa cgcgaatttg
    11101 ccaacgtggg catggccacc gcttacgggt tcggatccgg tatcggctgg atgctggcca
    11161 tcgttggtat ggcagccatc cgggagaaac tggaatattc ccacgttccc aaaccgctga
    11221 aaggattggg cataacattt atcatcacgg ccttgatggc cattggattc atgagtttct
    11281 cgggaatcaa tatttaacag taagctcaac gccaattaaa ataaacaaag ttttcaaaat
    11341 aaaaaaacag gatatacaat gaatgtgttg ttaatgagtt caggcggaat aaccatctta
    11401 accagtgtag tggttttctt gttgataatt ctgatcttgg taattgtcat tctggtggcc
    11461 aaagcaaagc tgatgccttc cggaaatgtg aagattaccg tcaataaaga gaaaaatcta
    11521 gaagttccca tggggtcaac cctgcttaat acgctgcaat cacagaacat attcatctcg
    11581 tccgcgtgtg gcggcggagg gacatgcgga caatgccgct gccaggtgct gaacggaggc
    11641 ggagaaatcc tcccgaccga aacc
//
LOCUS       DAOSDR010000038        11490 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrimonas sp. isolate AMR_MDS_1826
            NODE_2162_length_11490_cov_3.997989, whole genome shotgun sequence.
ACCESSION   DAOSDR010000038 DAOSDR010000000
VERSION     DAOSDR010000038.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863355
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrimonas sp. (wastewater metagenome)
  ORGANISM  Petrimonas sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Dysgonomonadaceae; Petrimonas.
REFERENCE   1  (bases 1 to 11490)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11490)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:10:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,720
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 35
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11490
                     /organism="Petrimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2162_length_11490_cov_3.997989"
                     /isolate="AMR_MDS_1826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2023866"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..759
                     /locus_tag="PLI30_02630"
     CDS             <1..759
                     /locus_tag="PLI30_02630"
                     /inference="COORDINATES: protein motif:HMM:NF025679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4382 domain-containing protein"
                     /protein_id="HOI78481.1"
                     /translation="GKAQIQFKLTDAPSLDYAKVVIDIQGVKVGVADEYYEDDNDPFY
                     DDTDELDEVEWVTLNISNPGLYNLLDYRNGEMIALAGGEIPAGKISQVRLLLGPDSYV
                     ETKDGTEYPLKTPSAQTSGLKFNLHETLLADMMYSFVIDFDAARSVVATGNNKYILKP
                     VIRTYAEAWGATLRGFVLPPEANAFVQITKATDTLISLPEEDGKFLFPGIDEGTWKVE
                     FIADTATEYTNFVMESVPVVKGEIKDVGTVTLVK"
     gene            870..2132
                     /locus_tag="PLI30_02635"
     CDS             870..2132
                     /locus_tag="PLI30_02635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005677586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3472 domain-containing protein"
                     /protein_id="HOI78482.1"
                     /translation="MNKICSTVLFCCSALFLFSQEVTYEIELNGNAYVTSFQQGASIT
                     RSGLEEWVNEKSVIKTFVYFHKTEKIVISIKGDSDKESVIEATFQGKRSKISVPAGRF
                     KIPLGSFQILNTGYQPIELRGISRSGKEFAQIESFVVQSEEQPTYVHDFSDYWGRRGP
                     SVHLRYTMPEDTVEWFYNEVTVPAGNDIPGSYYMANGFGEGYFGIQCNSETERRVLFS
                     VWSPFNTQDPKRIPDSLKIKLLRKGEGVYIGEFGNEGSGGQSFLRYNWKAGKAYKFLT
                     RIKPDGLGNTVYTSYFFASDENRWRLIASFLRPKTHVFYTRAHSFLENFIPGQGYLTR
                     KVLFGNQWFVTSNRKWIEAGESVFTYDETARKQVRLDYRGGYNKQQNLFYLQNGGFFN
                     KSTLYESRFTRKKENKIPKINFSELEKL"
     gene            2283..2795
                     /locus_tag="PLI30_02640"
     CDS             2283..2795
                     /locus_tag="PLI30_02640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019540860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HOI78483.1"
                     /translation="MKRSIYLMMALSAMLVLAISCKPKQSAYKQVYEAAKEREMQQTA
                     SQPTVVKDAGTLPPVEVSVRKEKVEPVLASDAANLKNFSVVIASLSVKLNAESLKTRM
                     QNEGYPVILAENEQGMYRVIVASYDDKASAAAKRDEIYQKYAALGNTDYLRRTYGVPF
                     NDLWILERQY"
     gene            2907..3665
                     /locus_tag="PLI30_02645"
     CDS             2907..3665
                     /locus_tag="PLI30_02645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004321259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOI78484.1"
                     /translation="MKVRFLGTGTSTGVPEIGCNCEVCTSVDAKDKRLRCSLQVETAD
                     SRLIIDCTPDFRQQVMDLPFRKIDGLLITHEHYDHVGGMDDLRPFCRFGPVDVFAETK
                     VKSALMQRIPYCFGEQRYTGVPDIRIRTTDGLHPFFINNTKVIPIRVMHFKLPILGFR
                     INNVAYLTDVKYLPDEELSKLAALDILIVSALRKEKHPSHQTLEEALSLAKKIGAEQT
                     YFTHMSHQIGLHKAISKDLPSSFTLAYDGLELSL"
     gene            3890..5209
                     /locus_tag="PLI30_02650"
     CDS             3890..5209
                     /locus_tag="PLI30_02650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI78485.1"
                     /translation="MKTKNLIISGLLVLAPVIGFAQEWDDIYADPAQNTTTLKVQKKQ
                     EPQKKKIVVVEGKASNMEVRANGRNIDEYNRREQKELAANDTVIVEDTAYQQYEYTDR
                     IIKYHDPESSIKITGADEVTVYVGDDIYADYYDNRGWGSNLYFGTGWGWNSYYPWYDP
                     WYDPWYYGYGWGYPYSNRWYSPWYYSRWHNPWYYGGWYSSWYSPWYYGGYYDPWYYGY
                     GGWYGGGYYSGFYDGYYSGLSNNRSGRSTGSYRRSSANIANRSSVASVGRSSSATSGR
                     SAIGSSTRTASGRSSSAYSRSSSASPRTRIIDNTGRVYDSRSGQIINRGSSSSRSSST
                     GTLGANSRIYNSRGSSSTSPARSGGNTYERSSSSRSNTYSTPSRSSSGSGSYSAPSRS
                     SSSSRSSSYDSSSRSSSFGSSSSGSSRSSSSGSYSSGSSGRSSGGRR"
     gene            5384..6958
                     /locus_tag="PLI30_02655"
     CDS             5384..6958
                     /locus_tag="PLI30_02655"
                     /inference="COORDINATES: protein motif:HMM:NF015316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein transport protein"
                     /protein_id="HOI78486.1"
                     /translation="MKKFAYFLFSLVFIANYSYSQGEVEALKYSRNELYGTARSMAMG
                     NAFGALGGDITGVSINPAGIAVYRSSEVVGTLGIQQNTAKVGDIDTKVSDFNLHNLGF
                     VGYFPLRNEVMPMINFGFSYNKQKTFNTDINAIGNAKSTMLDYMADRSSGVDATKLEM
                     RDNLPDPFIDQPWLSVLGFNSWLINDHMGTDGKYYYTPLDTKGEQAKQEIKSSERGYI
                     DNYDFTIGTTINNVVNVGLALNISDINHSLSTDFLEDFNSGGYTLNNEIITNGAGVGA
                     KLGVIYRPVNSFRIGLAYHTPTWYSMSEIYQARVDDDLGAYIDDPKYEKGWVNSAKFT
                     NHYDLKTPDKWVLSGAAVLGNSFILSADYEVMNYRNMKLSVPSGSYSSKDWYDIDNDY
                     IKKDFKVASTVRVGTEYRFTPQFSGRLGYAWMQNPYDADFSKAGDAAVSGSNTIYRME
                     GDTHYLTGGLGYRFNRNFYADLAVVYQTQEDQLYPFPNLFTYNGDTREELVIDASPFT
                     LKNRSVRGLLTLGYRF"
     gene            complement(7020..7802)
                     /locus_tag="PLI30_02660"
     CDS             complement(7020..7802)
                     /locus_tag="PLI30_02660"
                     /inference="COORDINATES: protein motif:HMM:NF025380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4230 domain-containing protein"
                     /protein_id="HOI78487.1"
                     /translation="MRKFKLVIGLTAMFLVLLLTGFIYATFFRPHYAVPENNTLGLLI
                     FGSIIGAVIAFLIFKLIYPSSSIGSSTNVTSHTVVESIRRVFKIVVAEGQLNEIYNYE
                     NTKKLLKFIPSTKKALVIVKAKVLIGYDIEKCKWEVDNENKIIKLLTFPAPEILSLDP
                     DFDYYYFEDDLFNFISRKDLQLIQGLAKEQVKQAALNSGLMKIASMQMKTLLTEMLNA
                     NKWQLEGVEKIEDYHVIPVAPQKSEISIGDNITLSEVDTLRK"
     gene            8042..8455
                     /locus_tag="PLI30_02665"
     CDS             8042..8455
                     /locus_tag="PLI30_02665"
                     /inference="COORDINATES: protein motif:HMM:NF019491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dabb family protein"
                     /protein_id="HOI78488.1"
                     /translation="MKHSDRRNFLKQTAALGAIAAAPVLTSCRSKGKGNDISGKFMHH
                     VFFWLKQPDDEKVKRDFREALIKLASVETVRFRHIGTPAATNREIIDTTYTFSLLVVF
                     DDENGHDVYQTHPIHDEFREKYANWWTRVLIYDTK"
     gene            8572..>11490
                     /gene="carB"
                     /locus_tag="PLI30_02670"
     CDS             8572..>11490
                     /gene="carB"
                     /locus_tag="PLI30_02670"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005840171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005951 - carbamoyl-phosphate synthase
                     complex [Evidence IEA];
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) large subunit"
                     /protein_id="HOI78489.1"
                     /translation="MDKSIKKIILLGSGALKIGQAGEFDYSGSQALKALREEGISTVL
                     INPNIATIQTSEGVADTVYFLPITPFFVEKVIQKEKPDGILLAFGGQTALNCGTELYQ
                     SGVLDKYDVKVLGTSVEAIMITEDRDLFVKKLNQIDAKTPVSQAVESMEDALKAAHTI
                     GFPVMVRSAYALGGLGSGICTNEEEFVTLCESALSFSKQILVEESLKGWKEIEFEVIR
                     DKNDHCFTVVPMENFDPLGIHTGESIVVAPIITLSREQIALLEDIARRVVRHIGIVGE
                     CNIQYAFNVETNDYRIIEINARLSRSSALASKASGYPLAFVATKLALGYSLDQIGEMG
                     TLNSAYVAPKVDYMIVKIPRWDLSKFAGVSREIGSSMKSVGEIMSIGQSFEEIIQKGL
                     RMIGQGMHGFVGNRNLTFDNVEEALSNPTDLRIFAIADAFEKGFTVDRIESLTKIDKW
                     FLEKLENIYNYTHVLTQYKKIEDLPKEVLAEAKRLGFSDFQIARYVENPQGTMEPELI
                     RVREWRKKQHVLPVVRRINTVASEDPEKTNYLYFTYGSDKAYQPVKSGKEVIIVLGSG
                     AYRIGSSVEFDWCSVNAVETARKLGYESVMINYNPETVSTDYDMCDRLYFDELTFERT
                     LDVIDIEVPKGVIVSMGGQIPNNLAMKLHRQNAPILGTSPESIDRAENRHKFSAMLDS
                     LGIDQPRWKELSSYEEVDKFVEEVGFPVLIRPSYVLSGAAMNVCYDKEQMHRFLEMAA
                     NVSKEYPVVVSVFMQNAKEVEMDAVAKEGEIVEYAISEHVEFAGVHSGDATLVFPAQK
                     IYFETMRRIKKISRQIAKELNISGPFNIQFLAKNNEIKVIECNLRASRSFPFVSKVIK
                     HNFIDTATRVMLGASYNKPDGTVFDLDHIGVKSSQFSYSRLQKFDPILGVDMASTGEV
                     GCIGDDFNEAILKSMLSVGYRIPRKGILISSGEVKSKVDLLEACKLLHAKGYDLYASH
                     G"
BASE COUNT         3442 a         2345 c         2637 g         3066 t
ORIGIN      
        1 ggaaaagctc aaattcagtt taaactgacc gatgccccat cattagatta cgccaaagtg
       61 gttattgaca tacagggcgt taaagttgga gtagcggatg aatattacga ggatgacaac
      121 gatccttttt atgatgacac cgatgaatta gacgaagttg aatgggttac cctgaacatt
      181 agcaaccccg gattgtataa tttgctggat tacagaaacg gagaaatgat cgccttggcc
      241 ggtggagaaa ttcccgctgg aaaaataagc caggtcaggt tactgctggg ccccgacagc
      301 tatgtggaaa ctaaggatgg aacagagtat ccccttaaga caccatctgc acaaacaagc
      361 gggttgaagt tcaatcttca cgaaacttta cttgccgaca tgatgtacag tttcgtcatt
      421 gattttgatg ctgcgcgttc ggtagtcgct acgggaaata acaagtacat tttaaaaccg
      481 gttattcgta cgtacgcaga agcttggggc gccacattga gaggttttgt cctcccgccg
      541 gaagccaacg cttttgttca aattaccaaa gcaacggaca cattgatttc gctgcccgaa
      601 gaggacggta aattcttgtt ccccggtatt gacgaaggta cgtggaaagt ggagtttatt
      661 gccgacacgg caacggaata tactaatttt gtaatggaga gtgttccggt tgttaaaggc
      721 gagataaaag atgtaggcac cgttacattg gtaaagtaat acaacaagta ttagcatact
      781 atcgagtaaa aaaaagcaag gccccttttc ccggggcttt gttgttttta ttggtcttta
      841 cacgtaactt tgcagataaa ttcaaacaga tgaataagat ttgctcaaca gttctctttt
      901 gctgttccgc cttatttttg ttttcgcaag aagtaacgta tgaaattgaa ttaaatggaa
      961 atgcttacgt gacttctttt caacagggtg catccatcac ccggtccgga cttgaagaat
     1021 gggtcaacga aaaatccgtc atcaagacat tcgtttattt ccacaaaacg gaaaaaatag
     1081 tgatttcaat aaaaggagat tcggataaag agtcagtgat tgaagcgact ttccaaggga
     1141 agaggtcaaa aatttctgtt cctgccggaa gatttaaaat tcctctaggg agctttcaaa
     1201 ttctcaacac gggttatcaa cccatagaat tacggggtat aagcaggtca gggaaagagt
     1261 tcgcccagat agaatcgttt gttgttcaat cggaggaaca accaacttat gtgcacgatt
     1321 tctccgacta ttggggtagg cgagggcctt ctgttcatct tcgttataca atgcccgaag
     1381 atacggttga atggttttat aacgaagtca ctgttccggc aggaaacgat atcccgggca
     1441 gttattacat ggcgaacggt tttggtgaag ggtattttgg aattcaatgc aattccgaaa
     1501 ccgaacgaag ggtgctgttc tccgtttgga gcccgttcaa cacacaggat ccaaaacgta
     1561 ttcccgacag tttaaagatc aaactcttac gaaaaggaga aggagtctat atcggtgaat
     1621 ttggaaacga aggttcgggc ggacaaagtt ttttgcggta caactggaaa gccggcaagg
     1681 cctataaatt cctgacccgg ataaagccag acggactggg gaacaccgtg tatacctctt
     1741 atttctttgc ttccgatgaa aatcgatgga ggctgatagc cagcttcctt aggcccaaaa
     1801 cccatgtttt ttatacccgt gcacattctt tcctcgaaaa ttttattcct ggacaaggat
     1861 acctgacccg aaaggtattg tttgggaatc aatggtttgt gaccagtaac cggaaatgga
     1921 tagaagccgg cgaatcggtt ttcacatacg atgaaactgc ccgaaaacaa gtccggttgg
     1981 attaccgggg cgggtacaac aagcaacaga acctgtttta tcttcaaaac ggaggattct
     2041 tcaacaaatc aactctttac gaatcaagat tcacaaggaa aaaggaaaac aaaattccga
     2101 aaataaactt ctccgaactc gaaaaactgt aactttagtc attggttctc ttgagtttct
     2161 gtctggagaa aagatgtttt taacaattta atttattttc acatctgtag gagttataat
     2221 catttactta tttctatctt tgcttttagt tttgatattc gaacaataaa acaatgacac
     2281 aaatgaaaag atcaatctat ttgatgatgg cgctttctgc catgttggtt ttagcaattt
     2341 cctgtaaacc aaaacaaagt gcatataaac aagtatatga agctgccaaa gaaagggaaa
     2401 tgcagcaaac ggcgtcacag ccaactgttg taaaagatgc cggcacactg cctccggttg
     2461 aagtgtcagt gagaaaagag aaggttgaac ccgttcttgc ttcagatgcc gccaatttaa
     2521 agaacttcag cgtagtaatc gcttctttaa gtgtaaagct taatgctgaa tcgttgaaga
     2581 cacgaatgca aaacgaaggg tatccggtga ttctggccga aaacgagcaa ggcatgtaca
     2641 gggttatcgt tgcaagctat gatgacaaag catcggctgc tgcaaaacgc gatgagattt
     2701 accaaaaata tgctgccctt ggcaataccg attacttgcg aagaacttac ggcgttcctt
     2761 tcaacgatct ttggatttta gaaagacagt attaaatgtt aaaacaccgg atccggatgt
     2821 ctggtgacca ataggaaatg aacgtataat aaagctgccc gctattgaaa cgggtagctt
     2881 ttattatttt acacggacaa taaaaaatga aagtacggtt tttaggtacg gggacatcta
     2941 caggagttcc cgaaataggt tgcaattgtg aagtatgtac atcagtcgat gctaaagaca
     3001 aacggttaag atgttctctt caagtggaga ctgctgattc acgccttatc atcgattgca
     3061 cacccgactt caggcaacag gtgatggatt tgcctttcag gaaaattgac gggttgctca
     3121 tcacccacga acattatgat cacgtgggcg ggatggacga cttaaggcct ttttgcaggt
     3181 ttggacccgt cgatgttttt gccgaaacta aagtgaaaag cgcgttgatg caacgaatac
     3241 cctattgttt tggagaacaa aggtacaccg gcgttccgga tatcaggata cgaacaaccg
     3301 acggccttca tcctttcttt ataaataaca caaaagttat tcctatccgg gtcatgcatt
     3361 ttaaactccc catcttaggt ttccggataa ataatgtcgc ttaccttacc gacgtaaaat
     3421 atttgcccga cgaagagcta agcaaactcg ccgccctaga tatactgata gtaagcgcat
     3481 tgagaaagga gaaacaccct tcacaccaaa cactggaaga agctctttcg ttggcaaaaa
     3541 aaattggagc ggaacaaacc tattttacac atatgagtca tcagatcggt ttgcataaag
     3601 cgataagcaa ggatttgccc tcatccttta cccttgctta tgacggatta gagttatcac
     3661 tgtaaaaaac ggggagcttt tgtatttttt taactacgta aagtgcagcg tttttataca
     3721 tttttcatct atataataaa caaatcgatg agttgacgtt taaaaatttg tatatttgtg
     3781 gatgataaat aacggaacag tgagttaaac caaatgaatg aattattgtc aaactctgcg
     3841 gaatgaatta tttaaaacaa ccaaaaacaa aaaataaagg gaaaaagcta tgaaaactaa
     3901 aaacttaatt atttcggggt tgctggtcct ggcgcccgtt ataggttttg cccaggagtg
     3961 ggatgatatt tatgccgatc ccgctcaaaa cactacaacc ctaaaggttc agaagaaaca
     4021 agaaccacaa aaaaagaaaa ttgttgttgt tgagggaaaa gcatccaata tggaagtccg
     4081 agcaaacggc cgcaatattg acgagtataa ccgtcgtgaa caaaaagaat tggctgccaa
     4141 tgacacagta attgttgaag atacagccta tcagcaatac gaatatacag acaggattat
     4201 caagtatcac gacccggaat cgagtatcaa gataaccggt gccgatgagg ttactgttta
     4261 tgtaggtgac gacatttacg ccgattatta cgataaccgt gggtggggct ctaacctcta
     4321 tttcggtaca gggtggggtt ggaacagcta ctatccgtgg tacgatccct ggtatgatcc
     4381 ctggtactac ggatacggat ggggttatcc ttattccaac cgttggtaca gcccgtggta
     4441 ttacagccgt tggcacaacc catggtatta cggtgggtgg tactcatcct ggtattctcc
     4501 ctggtactac ggtggttact atgatccttg gtattacggt tacggcggat ggtacggagg
     4561 tggttattat agcggtttct acgacggata ctattccgga ttaagcaaca atcgctccgg
     4621 cagaagtacg ggaagttaca gaagaagctc ggcaaacatt gctaacagat catctgtagc
     4681 aagtgtaggc agaagttcaa gcgcaacatc aggccgctcg gcgattggta gctccacaag
     4741 aactgcctca ggcagaagtt cctcggctta cagcagaagt tcatctgcat caccaagaac
     4801 aaggattatc gataacaccg gaagagtcta tgactcccgg tccggccaaa tcataaacag
     4861 aggctcatct tcttcccgct cctccagcac cggcacatta ggtgccaatt ccaggatata
     4921 taacagcagg ggtagttcca gcacctcgcc ggcgagaagc ggtggcaata cttacgaaag
     4981 aagctcgtcg agcagaagca atacctactc aacaccttcg agaagttcgt cgggcagcgg
     5041 ttcgtattcc gctccaagca gaagttcaag ctcaagtcgt tcgtcttcgt acgactcttc
     5101 aagccgcagc tcatcgtttg gttcctcctc atcgggttcc agtagaagtt cctcttccgg
     5161 ctcatatagc agcggaagca gcggtcgtag cagtggagga cgtcgttaaa ctataaacaa
     5221 taaacaatga ctgcttcaca aaaatctcag aaggggttta aatgaggcag tcatttttta
     5281 tccaacattt tgtaaaaccg tacgagcata tgcctggatt acttgtattt tgcctggcag
     5341 gaaagagcat aatttagtga aaacaaaaat aaaaactata aaaatgaaaa aatttgcata
     5401 ttttcttttc tctttagtgt ttattgccaa ctactcctac tcgcaaggag aagtggaagc
     5461 tttgaagtat tcccgaaatg agctttacgg aacagcacgt tctatggcaa tgggcaatgc
     5521 ttttggcgca ctgggaggag acatcacggg tgtttccatc aatcctgccg gaattgctgt
     5581 ttacaggagc tcagaagtag tgggtacgtt aggaatacaa caaaacacag ccaaggtggg
     5641 tgatatcgac acaaaagtga gtgatttcaa cctgcataac ctgggatttg tcgggtactt
     5701 tcccttgaga aacgaggtaa tgcccatgat taatttcggt ttttcgtaca acaaacaaaa
     5761 aacgttcaat accgatatca acgccatcgg aaacgccaaa agcaccatgt tggattacat
     5821 ggcagacaga agttctggag ttgacgctac caagctggaa atgcgagaca atttacctga
     5881 tccgttcatc gatcagccct ggctttccgt actggggttc aactcatggt taatcaatga
     5941 tcatatgggc acggatggaa aatattatta tactccttta gatacgaaag gagaacaagc
     6001 taaacaagaa ataaaatcat cggaaagggg atatatcgat aattatgatt ttaccatcgg
     6061 aaccactata aataatgtgg tgaatgttgg attagcactg aatatatctg acatcaacca
     6121 ctctctttct accgattttc tggaagactt taacagcgga ggttatacac tgaacaacga
     6181 gataataaca aacggtgccg gagttggtgc caagctgggg gtaatttacc gccccgtaaa
     6241 ttcattcagg attggcttgg cataccatac acccacatgg tattccatgt cggaaattta
     6301 tcaggcgagg gttgatgatg acctgggagc ttatatcgat gacccgaagt atgagaaagg
     6361 ctgggttaac tctgccaaat ttacaaatca ttatgatttg aaaactcccg ataaatgggt
     6421 attgagcggt gccgcagttt taggaaacag ttttatcttg agtgccgatt acgaagtgat
     6481 gaactatcgg aacatgaaac tgagcgtgcc ttccggtagt tattccagca aagattggta
     6541 cgatattgat aacgattaca tcaaaaaaga ttttaaagtt gcatctactg taagggtggg
     6601 tacagaatac cgttttactc cgcaattttc aggaagattg ggttacgcat ggatgcagaa
     6661 cccgtatgat gctgattttt ctaaagcagg agatgccgct gtatctggtt caaacaccat
     6721 ctacagaatg gaaggtgata cccattacct caccggagga ttgggttatc ggttcaacag
     6781 aaatttctat gccgatctgg cggtggttta tcaaacccag gaagaccagc tgtatccgtt
     6841 tccgaacctg tttacctata acggtgatac acgggaagaa ctagttattg atgcatctcc
     6901 ctttaccctg aaaaacagat ccgtaagagg attgctgaca ctcgggtaca ggttttagat
     6961 aattcatgtt aattttttag tgccgggctg tccttataga ttgcccggtt tttttgtggt
     7021 tatttgcgaa gggtgtccac ttccgacaag gtaatgttgt cacctatgga tatttctgat
     7081 ttttgtgggg caactgggat cacgtgataa tcctcaatct tctccacacc ttccagttgc
     7141 catttattcg cgtttagcat ttcggtcaac agggtcttca tctgcatcga tgcaattttc
     7201 atcaatccgc tgtttaatgc tgcctgtttt acctgttctt ttgccagccc ttgaatgagt
     7261 tgcaaatctt tccggctaat aaagttaaac aggtcatcct cgaaataata ataatcgaaa
     7321 tccgggtcaa gggaaagaat ctctggagcc gggaaggtga gtagttttat tattttattt
     7381 tcattatcca cttcccattt gcatttttca atatcataac ctatcagcac tttagctttt
     7441 acaatgacca gggctttttt ggtcgaagga ataaacttta gcagtttttt tgtgttctca
     7501 tagttataga tttcattcaa ctggccttca gctaccacaa ttttaaacac cctgcggata
     7561 ctttcaacaa cggtatggga agtaacgttc gttgaactgc caatactgct tgatgggtaa
     7621 atcaatttga aaatcaaaaa agcaataacc gcaccgatga tcgacccaaa aatcaataat
     7681 cccaacgtgt tgttttcggg caccgcataa tgcggtctga aaaaggtagc gtatataaat
     7741 ccggtgagaa gcagcacaag aaacatagct gtcaatccaa taacaagttt aaattttctc
     7801 attgaggctt tgtagattaa tgcagaggca aagttaagaa aatcttttcc ttggaaacaa
     7861 aagagttagc aaatattctt cttcagtcgg cgttttagtc aaaaaatgag atttaattcg
     7921 gttatttttg tgtaaatttg caaactaaaa cagtaaatgt acaaatactc cttcctttcc
     7981 ggttctacaa tccattcgtt gatttcctcc aagtcagcaa atcacttatt tttttatccg
     8041 tatgaagcat tctgacagaa gaaacttttt aaaacaaacg gcagctttgg gcgctattgc
     8101 tgcagcacca gtactgacaa gttgccgttc aaaaggaaaa ggaaacgata taagcggtaa
     8161 gtttatgcat cacgtttttt tctggcttaa gcagcctgac gatgaaaaag taaaacgtga
     8221 ctttcgcgaa gcgttaatta aactggcatc tgtggaaacc gttcggttca ggcatatagg
     8281 tactccggca gccacaaacc gtgaaattat tgataccaca tatacgtttt cactgcttgt
     8341 tgttttcgat gacgaaaatg gacacgatgt gtatcagaca catcccatcc acgacgaatt
     8401 ccgcgaaaaa tacgccaatt ggtggacacg tgtactgatt tatgatacaa agtagaaggt
     8461 gacaggcatc agccttttgg acgttcttcc gactgaaatc tgttagagaa gagagctgaa
     8521 gcctgaagtc agcaattaaa aacatacaga acgttttgaa caaaaataaa aatggacaaa
     8581 tcaatcaaaa aaattatctt attggggtca ggagcattaa agatcggaca ggcaggtgag
     8641 ttcgattatt caggatcaca agcattaaag gccttgaggg aggaaggaat aagcacggta
     8701 cttatcaatc ctaacattgc aaccatacaa acttccgagg gggtggcgga cacggtttat
     8761 ttccttccga tcaccccgtt ttttgtagag aaagtgattc agaaagaaaa acccgatggc
     8821 attttgctgg cttttggcgg acaaacggca ctaaattgcg ggacggaact gtatcaaagc
     8881 ggtgtactgg ataaatatga tgtaaaagtg ctgggcacct cagttgaagc gataatgatt
     8941 accgaagacc gggatttgtt tgtaaagaaa ctcaaccaga ttgatgccaa aacaccggtt
     9001 tcgcaagcgg tggaaagcat ggaagatgca ctgaaagcgg ctcatacaat tggttttccc
     9061 gtaatggtac gctcagctta cgcactgggt ggcctgggct cgggaatttg caccaacgaa
     9121 gaagagtttg ttacgctttg cgaaagcgcc ctttcgttct cgaaacagat tctggtggaa
     9181 gagagcctga aaggatggaa agaaattgag tttgaagtta tccgcgacaa aaatgaccac
     9241 tgttttacag tcgttcccat ggaaaatttt gacccgttgg gaatacatac gggagaaagt
     9301 attgttgtgg cacccatcat tactctttcc agggaacaga tagccttgtt ggaagacata
     9361 gcccgccgtg ttgtacgcca tatcgggata gtgggtgaat gcaacatcca atatgcgttt
     9421 aacgtggaaa ccaatgatta tcgcattatc gaaatcaatg cccgcctgag ccgctcatcg
     9481 gcactggcct ccaaggcttc gggttatccg ctggcgtttg tggcaaccaa gttggcattg
     9541 ggttattcac ttgatcagat cggcgaaatg ggtacgctca attcggctta tgtggcccca
     9601 aaagtggatt acatgattgt aaaaatcccc cgctgggact tgtctaaatt cgccggagtg
     9661 agtcgtgaga ttggctcatc catgaaatca gtgggtgaaa tcatgtctat cgggcaatcg
     9721 tttgaagaga tcatccagaa agggttgcgc atgatcgggc aaggtatgca cggctttgta
     9781 ggcaaccgca accttacatt cgataacgtg gaagaagcac tgtccaatcc tacggatttg
     9841 cgcatctttg ccattgccga tgcttttgaa aaaggattca cggtagaccg gattgaatcg
     9901 ctgacaaaga ttgacaaatg gtttctcgaa aagctggaga atatttacaa ctacacacat
     9961 gtgcttaccc aatacaagaa aatagaggac ctgccaaaag aggtccttgc ggaagcgaaa
    10021 agactgggat tctccgattt tcagatagcc cgttacgtgg aaaacccaca gggaacaatg
    10081 gaacccgaat tgatccgtgt gcgcgaatgg cggaaaaagc aacacgtctt gcctgttgtc
    10141 cggcgtatca acacagtggc atcggaagac ccggagaaaa ccaattatct ctatttcacg
    10201 tacggatcgg acaaagccta ccaacctgtg aaatcgggta aggaagtaat catcgtcctg
    10261 ggttcgggag cgtatcgcat cgggtcgtcg gttgagttcg actggtgttc ggtaaatgcc
    10321 gtagagactg cacgtaaatt gggttacgag tcagtaatga tcaattataa ccctgaaacg
    10381 gtatccaccg attacgatat gtgcgacagg ttatacttcg acgaacttac ttttgaacgt
    10441 acgctcgacg ttattgacat tgaggttccc aaaggagtaa tcgtatccat gggggggcag
    10501 attccaaata acctggccat gaagctgcat cgccaaaacg cgcccatttt agggacatct
    10561 cccgaatcga ttgaccgtgc ggaaaaccgg cacaagttct ctgccatgct cgatagcttg
    10621 ggtatcgacc agccgcgatg gaaagagctt tcgagctacg aagaagtgga taagtttgtt
    10681 gaagaagtag gattcccggt attgatccgt ccgtcgtatg tgctttcggg agcagccatg
    10741 aacgtttgtt atgacaaaga acagatgcac cgttttctcg aaatggccgc caacgtgtcc
    10801 aaagaatatc cggtagtagt ttctgttttc atgcaaaatg ccaaggaggt ggaaatggat
    10861 gctgtggcaa aagaaggcga aattgtggaa tatgccattt ctgagcacgt ggagtttgca
    10921 ggtgtgcatt caggtgacgc cacactggtt ttcccggcac aaaaaatata cttcgaaacc
    10981 atgcgccgca tcaagaagat atctcggcag attgccaaag agttgaatat aagtgggccg
    11041 tttaatattc agtttctagc taaaaacaat gaaataaagg tcatcgagtg taatttgcgc
    11101 gcctcgcgtt cattcccgtt tgtctccaaa gtcatcaagc ataactttat cgacacagca
    11161 acccgtgtaa tgctgggagc ctcttacaac aaaccggacg gcactgtttt cgatctcgac
    11221 cacatcggtg tgaaatcgtc acaattctcc tattcccgct tacagaagtt tgatccgatt
    11281 ttgggagtag acatggcctc gacgggagaa gtgggctgta taggcgacga ctttaatgag
    11341 gctattctta aatcgatgct ttcggtagga taccggattc ccagaaaagg gatcctgatt
    11401 tcgtcgggag aagtgaaatc gaaagtggat ttgttggaag cctgcaaact gctccatgcc
    11461 aaagggtacg acttgtatgc gagccacggg
//
LOCUS       DAOSDR010000039        11478 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrimonas sp. isolate AMR_MDS_1826
            NODE_2167_length_11478_cov_3.974350, whole genome shotgun sequence.
ACCESSION   DAOSDR010000039 DAOSDR010000000
VERSION     DAOSDR010000039.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863355
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrimonas sp. (wastewater metagenome)
  ORGANISM  Petrimonas sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Dysgonomonadaceae; Petrimonas.
REFERENCE   1  (bases 1 to 11478)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11478)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:10:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,720
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 35
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11478
                     /organism="Petrimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2167_length_11478_cov_3.974350"
                     /isolate="AMR_MDS_1826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2023866"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..284)
                     /locus_tag="PLI30_02675"
     CDS             complement(<1..284)
                     /locus_tag="PLI30_02675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005861367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HOI78490.1"
                     /translation="MLYYLFQYLDKLDFPGAGMFQFVTFRAALAALVSLLFSVIVGAR
                     IIRKLQKKQIGETIRNLDLEGQYSKRGTPTMGGVIIILSILVSILLMGKL"
     gene            complement(298..1758)
                     /locus_tag="PLI30_02680"
     CDS             complement(298..1758)
                     /locus_tag="PLI30_02680"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013063596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HOI78491.1"
                     /translation="MKLSQLTERCKIVAIHGEENVEVESITSDSRQVQKGSLFIAVEG
                     INTDGHDYIAKAIEQDVLVVVYDKPMFEEYFSRVTYVQVENSAVALAQIASVWYGNPS
                     EQLKLVGVTGTNGKTTVATLLYRMFRTLGYGAGLLSTVTNCVNDDSYPTTHTTLDPIT
                     LNSFLRKMVDAGCEYAFMEVSSHAIHQKRVYGLHFEGGIFTNLTQDHLDYHKNMLEYR
                     NVKKAFFDALPETAFALTNSDDKNGGVMLQNTKAKRYTYSVKGLADFKARIFEKHFDG
                     TEIEINGKQLIVQFVGVFNVYNLLAVYGVSILLGEEPEEVLRILSVLKPVAGRFQTLR
                     SPGNFTAIVDYAHTPDALTNVLNAIHEVLDQKGNIITVVGCGGNRDRTKRPLMAREAV
                     ELSNRVILTSDNPRFEEPQDIIDDMVAGLDDEQRRSALSIVDRREAIKTACALAKPGD
                     IILVAGKGHEDYQEIKGVKYHFDDKEELEKIFHTSP"
     gene            complement(1774..3972)
                     /locus_tag="PLI30_02685"
     CDS             complement(1774..3972)
                     /locus_tag="PLI30_02685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006841454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HOI78492.1"
                     /translation="MGKKTDENKKRILNRYGLIVFLLLGVGLMIIFSAGKIVFTAEGK
                     KWREVGERETIIKDRVILPKRGNIYTYDGKLLATSEPLYSVYMDFWAEGMRKDTLMKY
                     VGDLSVALAKKFPDRTASQYRNIILNGWKLREKEERQLQENATKGSEKKVALRSRYVR
                     ILRPDISYVDLKEIRTYPFLNQRSNRSGLIAEEKNARKRPFGNLANRTVGNVYKDLEK
                     GGASGLELKYDSLLRGIEGVKSRQKIQGRWTDIEEIAAKDGYDIVTTLDADIQDVTER
                     ALRAKLEETAAESGTAIIMETKTGEIKGIANLDRMSNGNYGEGNPNAFSYMNEPGSTF
                     KTVTVMVAIDDGLITPADSFHVGNGLYQYNGKWVRDHYWRQGRDRGYLTVKEGIEVSS
                     NVVMSKIVLKAYGDDPAKFVKGIDRIGLRKKLTWDVPLNGIEGTSSIRFPDDKVNYWS
                     KTTLPWMSFGYESKVPPIYMLMFYNGIANGGKMIKPFIAKTLLKDGKVVEEYKAEVVN
                     EKMCKDSTLIQIQDMLVGVIENGTAKVVKSDYFPIAGKTGTAQIASGGGYSDYYVSFC
                     GYFPADDPTYTCFVGIRKPRGVPSGGGQAGMVFKNIAEQTYVKRTQLAPEQARIDSTL
                     QKMPAIKSGSWGNSQAVLSMLNFKSSVSDSASDWVKVKYDSVAYHPQALAINKNSVPN
                     VLGMGAKDAVYLLEKSGLRVNLTGSGKVVSQSFTPESKLIRGTTISITLR"
     gene            complement(3974..4315)
                     /locus_tag="PLI30_02690"
     CDS             complement(3974..4315)
                     /locus_tag="PLI30_02690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019538612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsL-like putative cell division protein"
                     /protein_id="HOI78493.1"
                     /translation="MKVKLDNIRKSFVHVFGGNVLTENFFVRNLTFILVLVVIMILFI
                     SHRYTVLQRIAEMERLKVELKDAKYESLDISSDLTEASRQGQIEKRVEESGLELKINN
                     QPVYRIQKGKK"
     gene            complement(4343..5251)
                     /gene="rsmH"
                     /locus_tag="PLI30_02695"
     CDS             complement(4343..5251)
                     /gene="rsmH"
                     /locus_tag="PLI30_02695"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007216899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HOI78494.1"
                     /translation="MNLKIYHIPALLQESVGGLNIRPDGVYVDVTFGGGGHSKEILKR
                     LGKNGRLLAFDQDEDAYQNHIPDPRFTFVKSNFRFLKNFLRYHRIDEVDGILADLGVS
                     SHHFDDSDRGFSFRLPGDLDMRMNRLSRKTAADVLNEYSEEKLSDIFFFYGELKSARK
                     IATAVSAYRRSKKIGTVSDFLEIMRPFAFREKENKTLAQAFQALRIEVNDELEALEEM
                     LFQGLEMLKPGGRFSVISYHSLEDRLVKNFFKAGNFEGHIIKDFYGNRETPFELINRK
                     VIVPTEQEQVDNPRSRSAKLRIAEKI"
     gene            complement(5259..5702)
                     /locus_tag="PLI30_02700"
     CDS             complement(5259..5702)
                     /locus_tag="PLI30_02700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006841451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HOI78495.1"
                     /translation="MLQFLGNIEAKTDAKGRIFVPAVFRKRLQGAEEEFLVLRKDIFQ
                     DCLVLYPGTVWENEIETLRSRLNKWNKEQQQVFRQFVLDAERIEMDASGRILIPKRYM
                     VLVGIESEVRFLGVDNTIEIWAKEKLEKPLVDPDEFSERLQELMS"
     gene            5947..6783
                     /locus_tag="PLI30_02705"
     CDS             5947..6783
                     /locus_tag="PLI30_02705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005634271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HOI78496.1"
                     /translation="MSEKTDFKLDIDGVLQAKAKKHYKKIPKFVVNYLKRTIHQDELN
                     AIIERNRDKQGVDFMLALVDKEFKVNLKIHGEENIPAEGKFVFASNHPLGGLDGICLS
                     AYLGEKYKGKIKYLVNDVLLNIRNLESIFVPVNKYGSQAKLSAAAINEAYTSDNQIIT
                     FPAGLCSRKQNGRIKDLEWMKNFVVKAIEHKRDIIPVHFDAKNSDFFYNFANIRKSLG
                     LKFNIELIYLPGEMFKNKGQTFHITFGEPIPWQSLNKSKSPMQWAEEIKTVVYNLPQK
                     RD"
     gene            6790..7806
                     /locus_tag="PLI30_02710"
     CDS             6790..7806
                     /locus_tag="PLI30_02710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004380731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOI78497.1"
                     /translation="MEKIIDPIDKALIKNELTVGKRIRSTNKANNEIYIFTAHDSPNL
                     MREVGRLREIAFRHYGGGTGLEADIDKYDLMEHPYHQLIVWDPEKEEILGGYRFIMGK
                     KVDFDEKGKPMLATAHLLNFSDDFLQNYLPYTVELGRSFVSLEYQSTLHSRKGIFALD
                     NLWDGLGALTVIDPSMRYFFGKVTMYGTYNKEARNMILYFMHLHFPDPDKLVTPISQL
                     VTNTDTGKMKHLFKGKNFKEDYKVLNREVRSFGLNIPPLINAYMGLSPTMRFFGTSIN
                     DEFGDVEESGILIEINQILEEKKQRHIESYIKSNPSKRFLIGLRRAISGKSKNLIKKS
                     KNKK"
     gene            7913..8581
                     /locus_tag="PLI30_02715"
     CDS             7913..8581
                     /locus_tag="PLI30_02715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI78498.1"
                     /translation="MEAAMGWKEKADFCWFYYDTSSRPENSLASYMESHDEERAAFKQ
                     VAYAQTPLNSDLTARMNQLATNAAFFFPVPGPKMIWQFGELGYDVSIDENGRTGKKPI
                     KWDYFDVSQRKALHDTYVKLINLRKNHSELFISTATLDWKVSAGDWTGGRFITLSSFG
                     KNKQIVVVGNFTNAAMTASTTFPKTGTWYNYMNSPETLNVTSSTMNIAIPANSFRIYS
                     TFAP"
     gene            8735..9241
                     /locus_tag="PLI30_02720"
     CDS             8735..9241
                     /locus_tag="PLI30_02720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019539003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HOI78499.1"
                     /translation="MKSIAIIYGSSTENTKRAAEKIAERLSEYSPALIDIYDGDEEAF
                     HSNDVLILGISTWGVKDLQDDWSDFYHKLEKMDLTGKTVALFGLGDSFIYPDSFVDAM
                     GILYETVSEKGATTVGEVSPDGYQFDYSRALKNGVFVGLPLDEDNDADLTDERIEKWV
                     ESLKEHLS"
     gene            complement(9267..10607)
                     /locus_tag="PLI30_02725"
     CDS             complement(9267..10607)
                     /locus_tag="PLI30_02725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic 2,3-diphosphoglycerate synthase"
                     /protein_id="HOI78500.1"
                     /translation="MKRNAIIIGAAGRDFHNFNTFFRKNKDYRVVAFTAAQIPYIDGR
                     VYPAVLAGKELYPEGIPIVLESELPALIKEKNIHDCIFSYSDVPYPKLMHLSAVVNAA
                     GANFVLLGPNETMLTSTKPVIAVVATRTGCGKSQTSRAVVENLIKRGLKVIVVRHPMP
                     YGDLAAQAVQRFATVEDLKKHKCTVEEMEEYEPHVVRGNVIYAGVDYERILRRAEEDP
                     DGCDVILWDGGNNDFSFFKPDLTITVADPHRAGAEVNYYPGEVNLRLADAVVINKMDT
                     ASPEGVRTVRENIARVNPAAIVIDAASPVKADNPGLIRGKRCLIVEDGPTLTHGEMKI
                     GAGTVAAQKYGCAEEVDPRPFVVGELANTFAAYPHIGTILPAMGYSDRQLKDLETTIN
                     RADCDTVVIGTPIDLNRLIHIQKPVVRVHYDLQEIGTPTLNEVLDDFVKRCKLG"
     gene            10812..>11478
                     /locus_tag="PLI30_02730"
     CDS             10812..>11478
                     /locus_tag="PLI30_02730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019538632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase family protein"
                     /protein_id="HOI78501.1"
                     /translation="MSTSKLTLNVQSEIGKLEAVMLHYPGPEVENMTPKNAQRALYSD
                     ILNLSIAQKEYAQLKGVLEKTSLVYKVSDLLEKVLEIPGQRLSLLQKICVTEQVEDYF
                     DYLLELSSSQLTKILIEGLPMQVKTLTDFLKEEYYALQPLYNFYFTRDTSVTIGNTSL
                     VCKMANAVRMRESFIMDAIFNSGLFFNGKITNMYDASQNNPSVKIEGGDVLVAREDVL
                     LIGS"
BASE COUNT         3173 a         2851 c         2276 g         3178 t
ORIGIN      
        1 tctaatttac ccatcagcaa tatcgacacc aaaatagaga ggataatgat aacaccaccc
       61 atagttggcg ttccccgctt gctgtattgt ccttccaggt caaggttgcg aatggtttcc
      121 cctatctgct tcttttgtag tttacgaatg atcctggcac cgacaattac cgagaaaagc
      181 aacgaaacaa gggcggcgag tgctgcccgg aacgtaacaa actgaaacat tcccgctccg
      241 gggaagtcaa gtttgtccaa atattgaaat agatagtaca gcatatttag ttatgagtta
      301 tggtgacgtg tggaatattt tttccaactc ttctttatcg tcaaaatgat acttcacgcc
      361 tttaatttcc tggtagtctt cgtgtccttt tccggcaacg agaataatat cacctggctt
      421 ggccagtgca caagcggttt ttatcgcttc acgacgatcc acgatcgaaa gtgcactgcg
      481 gcgttgctca tcatccaaac cggctaccat gtcgtcaatg atatcctgcg gctcttcgaa
      541 ccggggatta tcggaggtca ggattacccg gttgctcaac tccaccgctt cacgcgccat
      601 taacggacgt ttggtcctgt cgcggttacc gccgcatcct actaccgtta ttatattgcc
      661 cttctgatcc agcacttcgt ggatagcatt gagaacatta gtcaaagcgt cgggtgtatg
      721 ggcatagtct acaatggcgg taaaatttcc gggcgatcgt aatgtctgga aacgtccggc
      781 cacaggtttc aaaacgctta atatcctgag tacctcctcc ggttcttctc ccagcaaaat
      841 agatacacca taaaccgcca gcaggttata cacattaaaa acacccacaa actgtacaat
      901 cagctgtttc ccgttaattt ctatttcggt cccatcaaaa tgtttctcga aaatccgtgc
      961 tttaaaatcc gccaatcctt ttacggaata agtatatcgt ttggcctttg tattttgaag
     1021 catcacccct ccgtttttat catccgagtt ggtaagggca aaagcggttt cgggcaaagc
     1081 gtcgaaaaaa gcttttttca cgttccgata ctccaacatg ttcttgtgat aatccaggtg
     1141 gtcctgcgtg aggttggtaa aaattccccc ttcgaaatgc agtccgtaga cccgtttttg
     1201 atgaatggca tgggaactca cttccataaa ggcatattcg caaccggcat caaccatctt
     1261 cctcaaaaaa gagtttaggg tgatggggtc aagggtggta tgcgtggtgg ggtaactgtc
     1321 atcgtttaca cagttggtta cggttgagag caagcctgcg ccatacccca atgtccgaaa
     1381 catcctgtac agtaatgtag cgacggttgt ctttccgttc gttccggtta caccaaccag
     1441 cttcaactgc tccgatgggt tcccgtacca gacagaagcg atttgcgcca aagcgacggc
     1501 cgaattttcg acctgaacgt acgtaactct cgaaaaatac tcttcaaaca tcggcttatc
     1561 atacaccacg accaacacat cctgttctat cgccttggca atataatcgt gtccatcggt
     1621 attgataccc tccactgcga taaaaagtga acctttttgc acctggcggg aatcggaagt
     1681 gatactttca acttccacat tctcttcccc gtgaattgcc acgattttac acctttctgt
     1741 aagttgactc agtttcatat taatatctgt tttttaccgc aacgttatgc taatggttgt
     1801 acctctaatc agtttgctct ccggagtgaa cgattgcgaa accactttcc ccgagccggt
     1861 cagattgacc cttaatcctg atttttcgag caggtaaacc gcatcttttg cacccatgcc
     1921 caacacattt ggcaccgaat tcttatttat cgccaatgcc tgtggatgat aagcaacgct
     1981 gtcgtatttt actttcaccc agtcggaagc ggaatccgaa acggaagatt tgaaattcag
     2041 catcgacagt accgcctgac tgtttcccca gcttccgctt ttaatagccg gcattttctg
     2101 cagggtagaa tctatccgtg cttgttcagg ggcaagctga gtccgcttaa catacgtctg
     2161 ctccgcgata tttttgaaaa ccatacctgc ttgtcctccg cctgacggaa ctcctcgagg
     2221 ttttctgata cctacgaaac acgtataggt tgggtcgtct gcggggaagt atccgcagaa
     2281 cgaaacgtaa taatcgctgt atccgccacc actggctatt tgtgctgttc ccgtttttcc
     2341 tgcaataggg aaatagtccg atttcacaac tttcgccgtc ccgttctcaa taacccctac
     2401 cagcatatcc tggatttgga tcagggtgct atctttacac atcttttcgt tcaccacttc
     2461 cgccttatac tcttccacca cttttccgtc tttcaacaat gttttagcaa taaacggctt
     2521 gatcattttt ccaccgttgg caataccgtt gtagaacatc agcatataga tgggtggaac
     2581 tttggattca taaccgaaag acatccacgg cagtgtggtt ttggaccagt aatttacctt
     2641 atcatcggga aaacgaatgg atgatgttcc ttcaattccg ttcaacggga catcccaggt
     2701 gagttttttt cgtaagccta tcctgtcaat gccttttaca aattttgccg gatcatcacc
     2761 gtatgcttta agtacaattt tactcatcac cacgtttgaa gagacttcta tcccttcctt
     2821 taccgtaagg tatccccggt cacggccctg tcgccaatag tggtcacgca cccactttcc
     2881 gttgtactga tacagtccgt tgcccacatg aaaggagtcg gcaggagtta tcaatccgtc
     2941 atcgattgcc accatgaccg ttacggtctt gaaggtagat cccggttcgt tcatgtagga
     3001 aaaagcattg ggattaccct caccatagtt cccgttactc atccggtcca agttggcaat
     3061 ccccttaatc tcacctgttt tggtttccat aataattgcc gtccccgatt cagccgccgt
     3121 ctcttcaagc tttgcccgga gcgcacgctc cgtcacatcc tgaatatcgg catcgagcgt
     3181 agttacgata tcgtatccgt ctttagcggc aatctcctct atatcggtcc agcggccctg
     3241 tattttttgt cggctcttca ccccttcaat ccccctcaac agggagtcgt attttaattc
     3301 cagtccgctt gctccccctt tctcaaggtc tttgtatacg tttccaaccg ttctgttggc
     3361 gaggttcccg aaggggcgtt tccgcgcatt tttttcctcg gcaattagtc cgctgcggtt
     3421 ggaacgctgg ttgaggaacg gataggtacg aatctctttc aagtcaacgt aggagatgtc
     3481 agggcgaagg atacgcacat agcgcgacct cagcgctacc ttcttctcac tgccttttgt
     3541 ggcattctcc tgcagttgcc gctcctcctt ttcgcgtagt ttccatccgt tcaggataat
     3601 gtttctgtat tgcgaggccg ttctgtcggg aaacttttta gcgagggcta ccgaaaggtc
     3661 accaacatac ttcatcaacg tatcttttct catcccctct gcccagaaat ccatgtacac
     3721 actgtacaaa ggctcactgg tagccagaag cttcccatca taagtataga tatttcctcg
     3781 cttaggaaga atgacgcggt ctttgataat ggtttctctt tcccctactt cccgccactt
     3841 ctttccttcc gccgtaaaaa cgatctttcc tgcagagaaa ataatcatta gcccaactcc
     3901 cagaaggagg aatacaatta agccgtaacg atttaaaatc ctctttttgt tttcgtcggt
     3961 tttctttccc atttcatttt ttccctttct gaatgcgata aaccggttgg ttatttattt
     4021 tcaactctaa acccgactct tccacccttt tttcgatttg tccctggcgg ctggcttcag
     4081 tcaagtcgga tgaaatgtct agcgactcat acttagcgtc cttcaactca actttcaggc
     4141 gctccatctc tgctatacgc tgcaaaaccg tgtaccggtg gctgatgaac aaaatcataa
     4201 tgacaaccaa aaccaaaata aatgtaaggt tcctgacgaa aaagttctcc gtcaacacat
     4261 ttccaccgaa aacgtgtacg aatgattttc ttatgttgtc tagcttcact ttcatcactt
     4321 tgtgaggtgc aaactccctc cattatattt tctctgcaat tcgcagtttg gcacttctcg
     4381 aacgcggatt gtccacctgc tcttgttccg ttggaacaat cactttcctg ttgatcaatt
     4441 caaacggggt ttccctgtta ccgtaaaaat ccttgatgat atgcccctcg aaatttccgg
     4501 ccttaaagaa attcttcact aaccggtcct ccagtgaatg ataggaaata acactgaacc
     4561 gcccaccagg tttcaacatt tccagtccct gaaacaacat ttcctccagc gcctccaact
     4621 catcattcac ctcaatacgc aacgcctgaa aagcctgagc cagggtttta ttttcttttt
     4681 ctcgaaaggc gaacggcctc ataatctcca ggaaatcaga aacagtaccg atctttttcg
     4741 accggcgata agcagaaacg gccgttgcaa tcttccgcgc acttttcaat tccccgtaaa
     4801 agaaaaaaat atccgacaac ttctcttccg aatattcatt caaaacatca gcggctgttt
     4861 ttctggacag ccggttcatc cgcatatcca gatctcccgg caaccgaaag gaaaatccac
     4921 gatccgaatc gtcaaaatga tgcgacgaca cccctaaatc ggcaagaatt ccatccacct
     4981 catctatccg gtgatagcgc agaaaattct tcaaaaatcg aaaattactt tttacgaatg
     5041 tgaaacgcgg gtccgggata tggttttggt atgcatcttc atcctggtca aaggcaagca
     5101 gtctgccatt ctttcccaac cgcttcaata tttctttcga gtgcccacct cctccaaatg
     5161 tcacgtcaac ataaacccca tcgggacgga tattcaaccc gcctacactt tcttgaagca
     5221 atgccggtat gtggtatatt tttaaattca tggacacatc aggacatcag ctcttgcaac
     5281 ctttctgaaa actcatcggg atcaaccaac ggcttttcga gtttttcttt cgcccatatc
     5341 tcaatagtat tatccacacc cagaaaacga acttcagatt cgatacccac cagcaccata
     5401 tagcgcttag ggattaaaat tcgcccgctt gcatccattt caatccgttc ggcgtcgagc
     5461 acaaactgac ggaaaacctg ctgttgctcc ttgttccact tgttgagccg gctcctgagt
     5521 gtttcgatct cgttttccca gactgtaccg ggataaagta ccaaacaatc ctgaaaaata
     5581 tccttgcgaa gcaccaaaaa ctcttcttct gcaccctgca gacgcttacg aaaaactgcc
     5641 ggcacaaata tccgcccctt cgcatcggtt tttgcttcaa tatttcccag gaattgcaac
     5701 actatattct taattctatg atttttgcgg taaaattaca aaataaatcc actttccccc
     5761 acttttccac acattatttt taataaagtt atcaacaagt tctttaaatg ctgattagat
     5821 gcaacttaca cactatttaa tcccataaaa tagtgtagaa tatttctacc gttttaaata
     5881 aatgagatct gcttgtcctt tattcttaat tattttgtat ttttgtaagt ccgtaggaaa
     5941 gtcattatgt cggaaaaaac tgatttcaaa ttagatatcg atggtgtttt gcaggctaaa
     6001 gcaaaaaaac actacaagaa aatccccaaa tttgtcgtta attatttaaa gcgcaccatt
     6061 catcaggatg aattaaatgc gatcatcgag cgtaaccggg ataagcaggg tgttgatttt
     6121 atgctcgccc tggtggataa agagtttaaa gtcaacctaa aaatccacgg ggaagaaaat
     6181 attcccgcgg agggaaaatt tgtttttgct tccaatcacc cgctgggtgg cctggacggg
     6241 atttgcctat cggcttattt aggggaaaaa tataagggga aaatcaaata cctggttaac
     6301 gatgttttgt taaacatcag gaacctcgaa tccatttttg ttccggtcaa caaatatggt
     6361 tcacaggcca aactatcggc cgctgccata aacgaagcct acacgtcaga caaccaaatc
     6421 attaccttcc ccgccggatt gtgttcccga aaacagaacg gacggataaa ggatctggaa
     6481 tggatgaaaa actttgtcgt aaaagctatt gaacacaaaa gggatattat ccccgtccat
     6541 ttcgacgcga aaaactcaga cttcttttat aattttgcca atatccgcaa atcattggga
     6601 ctaaaattca atatagaact tatatacctg ccaggcgaaa tgttcaagaa caaaggtcaa
     6661 acattccaca tcacttttgg agaacccata ccctggcaat cgttgaataa atcaaaaagt
     6721 cctatgcaat gggcggaaga gataaaaacc gttgtttaca atttacccca aaaaagagat
     6781 taattaagta tggaaaaaat catcgatccg attgataaag ccctcataaa aaacgaacta
     6841 accgtaggaa aaagaatacg atccaccaac aaggccaaca acgaaattta catcttcact
     6901 gcccacgatt cgcctaatct catgagggaa gtggggcggc tacgtgaaat tgctttccgt
     6961 cactatggag gaggaaccgg actagaagcc gatatcgaca aatacgacct tatggagcat
     7021 ccgtatcatc aacttattgt ctgggatccg gaaaaggaag agattctagg cggataccgc
     7081 ttcattatgg gtaaaaaagt agattttgat gagaagggta agccgatgct cgccaccgct
     7141 catttattaa acttctccga tgatttcctg caaaactact tgccgtacac cgtagaatta
     7201 ggccgatcgt tcgtctctct cgagtatcaa tctaccttac acagccgcaa aggcatcttt
     7261 gcacttgata atctttggga cggcctgggc gcactcacgg taattgatcc ttcaatgagg
     7321 tattttttcg gaaaagtaac catgtacggg acctataaca aagaagcccg caacatgatc
     7381 ttgtatttta tgcacctgca ttttcccgac ccggacaaac tagtcacccc aatcagccaa
     7441 cttgtcacca ataccgatac aggcaagatg aaacacctgt ttaaaggtaa aaacttcaag
     7501 gaagactaca aggttctgaa ccgggaagtc cgcagcttcg ggttaaacat tccaccgctc
     7561 atcaatgcct acatgggatt gtcacccacc atgcgtttct tcggaacttc cataaacgac
     7621 gaattcggag acgtggaaga atcaggtatt cttatcgaga tcaaccagat cctcgaagag
     7681 aaaaaacaac gtcatattga atcctatata aaatccaacc caagcaaacg attccttatc
     7741 ggattacgga gagccatttc cggaaaaagc aaaaacctga tcaaaaaatc caaaaacaaa
     7801 aagtgaaaac ctccgtcttt ttcctaccat aaggcactcg ttcaaaaacc gtaaattact
     7861 gatagtttat tttttacact taaaaaatgc aatcgtttgc actgaagctt atatggaagc
     7921 agctatggga tggaaggaga aagcggactt ctgctggttc tactacgaca cctcttcacg
     7981 cccggagaac agcctggcaa gctatatgga aagtcacgat gaagagcgtg ctgcttttaa
     8041 acaggtggct tatgcccaaa cgcctcttaa ttcggattta acagccagaa tgaatcaatt
     8101 ggctacaaac gcagctttct tcttcccggt tcccgggccc aagatgatat ggcaatttgg
     8161 tgaattagga tacgatgttt caattgatga aaacggacgc accggtaaaa aaccaatcaa
     8221 gtgggattat tttgatgttt cgcaacgaaa agctcttcat gacacatatg taaaactgat
     8281 caacttgaga aagaaccaca gtgaattgtt tatatctacc gctacattag actggaaagt
     8341 ttcagcaggc gactggaccg gaggtcgttt tatcaccctt tcctcattcg gaaaaaacaa
     8401 gcagatagtt gttgttggca atttcacaaa cgcagccatg accgcgtcaa ccactttccc
     8461 gaagacaggg acatggtaca attacatgaa cagtccggaa acccttaacg taacctcttc
     8521 aacgatgaac attgctattc ccgccaacag tttcaggatc tattccacgt ttgcaccgta
     8581 aacaaactat ttccggaaaa ggaaatctac aaaagcgtac tgcggctcaa aaccgcttgt
     8641 acgctttctt gttctcgggc ataaattcct ttctgtttgc gaatatgaaa ctaaatgtta
     8701 actttgcaaa atcatttttt aatcacgtaa gcttatgaag tcaattgcaa ttatatacgg
     8761 ctccagcacc gagaacacaa aaagagctgc ggagaaaatc gctgaaagac tatcggaata
     8821 ctctcccgcc ctgatcgaca tttacgacgg tgatgaagaa gcttttcatt ccaacgatgt
     8881 acttatcctc ggcatttcga cctggggagt aaaggatttg caggatgact ggtctgattt
     8941 ctaccacaag ctggaaaaaa tggacctgac ggggaaaaca gttgctcttt tcgggttggg
     9001 cgactcattc atttatcccg attctttcgt ggatgcgatg ggtattttgt acgaaacagt
     9061 ttcggaaaaa ggagcaacaa cagttggtga agtttctccc gacggctacc aattcgatta
     9121 ctccagggca ttaaaaaacg gcgtttttgt gggattaccg ctggatgaag acaacgacgc
     9181 cgacctcacc gacgaacgca tcgaaaagtg ggtggaatcg ttgaaggagc acctcagtta
     9241 aaccggcctg cccccggcaa acccttttat cccagcttac atcgcttcac aaaatcatcc
     9301 aatacctcat tcaacgtagg agtgcctatt tcctgcaaat cgtagtgaac ccgaacaacg
     9361 ggcttctgaa tgtggatcaa ccgattcaag tcgatgggcg tgccaatgac aaccgtatcg
     9421 caatctgcac ggttaatggt tgtttccagg tccttcagtt gccggtcgct ataacccatg
     9481 gccggcagaa tagtgccgat atggggatag gcagcaaaag tatttgctaa ttcgcccacg
     9541 acaaaaggac gcggatcaac ctcttccgca catccgtatt tctgtgccgc aacggtaccg
     9601 gcaccgatct tcatttcgcc gtgagtcaat gttggcccat cttcgacaat caggcaacgc
     9661 tttcctcgga ttaatccggg attatctgct ttaacaggac tggctgcatc gatcactatg
     9721 gctgcaggat ttaccctggc aatgttctca cgaacagtcc ggacaccttc cggagaagcc
     9781 gtatccattt tattgatgac tactgcgtcg gcaagcctta aattcacttc cccggggtaa
     9841 tagttcactt cggctccggc acgatgcgga tcggccacgg tgatggtaag atcgggttta
     9901 aaaaacgaga agtcgttgtt cccgccgtcc cacaaaatca cgtcgcaacc atcggggtct
     9961 tcctcagccc gccgcaaaat cctttcgtaa tcgacgcctg cgtaaataac gttacccctg
    10021 accacatggg gttcatattc ctccatttct tcgacggtgc atttgtgctt tttcaaatct
    10081 tccaccgtgg caaatcgttg cacagcctgg gccgccaagt caccgtacgg catcggatgg
    10141 cgtacgacga tcacttttaa cccacgcttt atcaggtttt ccactacagc tctagaagtc
    10201 tgagacttac cgcaccccgt gcgtgtagca acaacagcta tcacaggctt agtactcgta
    10261 agcatggttt cattcggccc cagcaaaaca aagttcgctc cagctgcgtt taccaccgca
    10321 ctcaagtgca tcaatttcgg ataaggcacg tcgctgtatg aaaaaatgca gtcgtgtata
    10381 tttttctctt tgatcaacgc cggcaattcc gattccagca caatggggat tccttccgga
    10441 taaagttctt ttcccgcgag aacggcagga taaacccttc cgtcgatata cggaatctgt
    10501 gctgctgtaa aagcaaccac acggtaatcc ttattcttcc tgaaaaatgt gttgaaatta
    10561 tggaagtccc ttccggcagc tccaatgata atggcatttc ttttcataac aatgaagatt
    10621 aaatgagtta attctctgaa acaaaggtat aataattttt atcaaaaaga taattattcg
    10681 gtttgttttt ttcaaaacga caactcatgt ctgacatact gaaaactttc aattataaaa
    10741 atacacgtgt ctaaaaaaca ggataactta aattttacta tttttgtgtt ttaaatcaaa
    10801 tcttacgtat tatgtctacg agcaaactca ccttaaacgt tcaatcggaa atcggaaagt
    10861 tggaagcggt aatgttgcac tatccgggtc ctgaagtgga aaacatgaca ccaaaaaatg
    10921 ctcagcgggc actctacagc gacatcctta acctctctat cgcacaaaaa gaatatgccc
    10981 agttaaaagg tgtactggaa aagacttctt tagtatataa agtttccgac ctgcttgaaa
    11041 aagtgctgga aattcccggg caacgattat cgttgcttca aaagatctgt gtgacggaac
    11101 aggtagaaga ttatttcgat tatctcctgg aattatcctc atcccagtta acaaaaatat
    11161 tgattgaagg acttccgatg caggtaaaga ccctgaccga ctttctgaaa gaagagtatt
    11221 atgcgttgca gcccttatac aatttttatt ttacgcgcga cacatcggta accattggca
    11281 acacctcgtt ggtttgtaag atggccaatg cggtgcgcat gagagaatcg tttatcatgg
    11341 atgcgatttt caatagcggg ttgtttttca acggcaagat cactaacatg tatgatgctt
    11401 cacaaaacaa cccctccgtg aaaatagagg ggggcgatgt attggtcgca cgtgaagatg
    11461 tgctgttaat cggaagcg
//
LOCUS       DAOSDR010000040        11433 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrimonas sp. isolate AMR_MDS_1826
            NODE_2176_length_11433_cov_4.513271, whole genome shotgun sequence.
ACCESSION   DAOSDR010000040 DAOSDR010000000
VERSION     DAOSDR010000040.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863355
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrimonas sp. (wastewater metagenome)
  ORGANISM  Petrimonas sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Dysgonomonadaceae; Petrimonas.
REFERENCE   1  (bases 1 to 11433)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11433)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:10:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,720
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 35
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11433
                     /organism="Petrimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2176_length_11433_cov_4.513271"
                     /isolate="AMR_MDS_1826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2023866"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..436
                     /locus_tag="PLI30_02735"
     CDS             <1..436
                     /locus_tag="PLI30_02735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006841506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycoside hydrolase family 16 protein"
                     /protein_id="HOI78502.1"
                     /translation="TWPAFWMLPEKEQLNWPLDGEIDIMEYVGYDPGWIHASVHTKAY
                     NHTIGTQKTARKEVKTAETAFHIYAVEWTADYIKGFVDGVEYFRFNNDGAGNKETWPF
                     NVPFYLKLNLAWGGNWGGAQGVDESKLPATYEIDYVRVYQKK"
     gene            501..2846
                     /locus_tag="PLI30_02740"
     CDS             501..2846
                     /locus_tag="PLI30_02740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019540032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HOI78503.1"
                     /translation="MNTNKIFFFSFFILLLTACNGGTGKTGMTLDTGVEKKIDSILSQ
                     MTLEEKVGQMAQFTIDLIGKGGNAYFSNEPFEVDPAMLDTVIGKYKVGSILNTSNNRA
                     RTTEVWENTIRTIQQRALQETGIPVIYGIDAIHGTTYTAGGVLFPQAIGMAATFNTGL
                     MKEGSRITAYETRASNIPWTFAPTMDLGRDARWSRQWESYGEDAYLNAQMAVASTLGF
                     QGEDRNNVGSQHVAACLKHYMGYGVPVSGKDRTPTVIPENDLREKYFEPFRASIEAGA
                     LSVMVNSGMINGVSTHVDYRLLTQWLKEDLDFDGVIVTDWADVQNLLSRDRVAADYKE
                     AVKKAINAGIDMVMEPYNLAFCPTLIQLVQEDQVPMKRIDDAVRRVLRLKYRLGLFDR
                     PYWPREEYPDFGNSGNEEAAKAAADESITLLKNDNNILPLPPAARILVAGPNAHSMRT
                     LNGGWSYSWQGEKVEEFAQDYNTIYEALQQKFGASNVRYEPGVTYKMDGQYFEENAPE
                     IAKAVTAAGGADYIVLCLGENSYCETPGNLDELTLSENQTELALALQKTGKPLILVLN
                     EGRPRLIRKIEPGAMAVVQTYLPGNFGGDALADILSGDVNPSGKLPYTYPKYEQGLIT
                     YDHKPSQNIDGKMEGAYDYGAQTSVQYPFGYGLSYTSFDYSNLRVSNTLFTAEDVFSV
                     SVDVTNSGAREGKEAVLLFSSDLVASLSPDVRRLRAFEKISLKPGETRTVTFEMKGNE
                     LAFVNEQGHWVLEEGEFMLQAGNQTAGIRCTATKIWETPNK"
     gene            complement(3053..5218)
                     /locus_tag="PLI30_02745"
     CDS             complement(3053..5218)
                     /locus_tag="PLI30_02745"
                     /inference="COORDINATES: protein motif:HMM:NF024991.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HRDC domain-containing protein"
                     /protein_id="HOI78504.1"
                     /translation="MEKMSYLSPYECAKEFVTYTGRNIFITGKAGTGKTTFLHRFRTE
                     TTKQVAVVAPTGVAAINAGGATIHSFFQLPFTPFTPTPAGCEALIARMKMNSFRRRVI
                     RELEVLVIDEVSMVRADVLDAIDTVLRSVRHRRSEPFGGVQVVFIGDVYQLSPVAKAD
                     EWRILSDYYRGIYFFHSRVIQEHPPVYVEFDKIFRQSDNLFIDVLNEVRNDALSPEGL
                     ELLQSRYDPHFNPTPEENYITLTTHNFSADAINSTELEKINTTAHSFHAVVKGEFPDN
                     AFPVDRVLILKEGAKVMFVKNDTEVPRRYFNGKIGTVTHILEEGVTVQCPDDTEEITV
                     SPVLWENIRYTTHSETNTVEEEIIGTYKQIPLRLAWAITIHKSQGLTFDKAIIDAGKA
                     FSPGQVYVALSRCRSLDSLVLKSPINRYSIGVDEQVVRFSSSKPEENQVAGELQLAKK
                     QFSINLLLQLYDFDPLLQAARSWYSNTQENESSFSEGTVSFVSEVCNQLTELEQVAGK
                     FRIQLQHITGQTPVNKVFFAERLRASSSYFTEKIETLLRTLQESTATTDSKANAQEYD
                     EDIVSLFVAAALKAHLISATSDNFCIESYYNARRQFRQPPFSLTSYSRDSTGIQLKSI
                     HPELLSELVQLRNRISKEENLPVYIVASVKTLVQMADYLPETEKELLRIHGFGKVKTE
                     RFGAKFLELIQNYIAAYGIESRMIQFKEDKKQRKRKNKG"
     gene            complement(5218..6459)
                     /locus_tag="PLI30_02750"
     CDS             complement(5218..6459)
                     /locus_tag="PLI30_02750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009126568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyloxyacyl hydrolase"
                     /protein_id="HOI78505.1"
                     /translation="MNKQITYKLLIFATVFLFFATKPSSAQTRSDSVLQPDRFRNRLE
                     FEARPGYIIPTHNFLKGENARSKPIRNSFSTHLKYSFQFKPGSPAGSIYGIPHQGIGV
                     AYFTFGEPQQLGAPLAVYLFQGARLAQMTSKLSLHYEWNFGLSGGWKPYHPKTNPDSR
                     VIGSKINAYMNTGFYLNQTLSQYFNLIGGISIAHFSNGNTQFPNAGLNTAGVKLGLMY
                     TLNPSIMSRQSPYRGAVADFPRHVSYDIVLFGSWRRKGVYLEGKGIASPHEYPVGGFN
                     INPMYNFGYKLRAGLSLDGIYDSSANVYTEDYIVEPGGKNPGYTFHNPPVNKQLALGI
                     SARAEYVMPYFTVGVGIGSNIVHGGGDLAGLYQMLALKVEVTRNSFIHIGYNLQNFQT
                     PNYLMLGIGYRFNNKYPCFYR"
     gene            complement(6497..8290)
                     /gene="argS"
                     /locus_tag="PLI30_02755"
     CDS             complement(6497..8290)
                     /gene="argS"
                     /locus_tag="PLI30_02755"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008779257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HOI78506.1"
                     /translation="MNIENILQTAIISAIKELYSVDVNASQITLQKTKKEFKGHFTLV
                     TFPLLKISKKNPEQTAQEIGMYLADKSPVVSEFNVIKGFLNLTIASKVWLDLLENIDK
                     NPEFGFTPVANDAPLFMVEYSSPNTNKPLHLGHIRNNLLGHSLCEILKANGKRVVKTN
                     IVNDRGIHICKSMLAWQKWGDGETPASSGKKGDHLIGKYYVLFDKKYKAELADLQAAG
                     LSREQAEAQSVLMEEAREMLRQWEANDAQTVNLWKMMNGWVYAGFDETYEKLGVSFDK
                     IYYESQTYLAGKDEVLRGLKEGVFYQREDGSVWADLTHEGLDEKILLRSDGTSVYMTQ
                     DIGTAKLRFDDYPIDTMIYVVGNEQNYHFQVLSILLDKLGFEFGKGLVHFSYGMVELP
                     EGKMKSREGTVVDADDLMAEMIQTARETSEELGKLDGVTPEEADRVVTMIGMGALKYF
                     ILKVDPKKNMTFNPKESIDFNGNTGPFIQYTHARIKSVLRKAEEQGIPVPESMQADIV
                     LSEKEEGLVQLLAEFPDIVKQAGDEYNVSLIGNYVYDLAKEFNQFYHDFPMLREKDEA
                     LRAFRLLLSKNVAITIKKGMVLFGIEVPERM"
     gene            complement(8332..11163)
                     /locus_tag="PLI30_02760"
     CDS             complement(8332..11163)
                     /locus_tag="PLI30_02760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019538416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding and (Fe-S)-binding domain-containing
                     protein"
                     /protein_id="HOI78507.1"
                     /translation="MENKQLHSRLTKLFPKEQVFTDELSRLVKGTDAGLYRLVPKAVV
                     KVNTEDEVIRLLQFCSKENVPVTFKAAGTSLSGQTVSDSILMEIGQGFEFSVITENGK
                     TATFGAGLTGTAANRMLQRYRRKLGPKPASINSAKIGGIIANNASGSSYGIKHNSYHT
                     VKSMRIIFADGSLLDTADTTSCQSFIASHPEFIAQIERLHNEASGNEGVKNRIQQKFQ
                     LKNTCGYGVNSLIDFSDPVDILQHLMIGSEGTLGFVSQATFETVHDAPLKATAMLYFH
                     NLRDVCETILPLRSCSVSAAELMDRNALRAVENQEGMPAELKSLPEGAAALLIDTSAD
                     DEETLLVQMRDIEAKLAHIQTLYPIRFTTDIHLYNLYWNVRNGLFTSAAASRPPHTAS
                     IIEDIAFSGEILGDALTDVRELLVRTGYADAVMWGHLLDGNVHFTVFPDINSPDGVDK
                     YARFMHELANLVAVKHNGSLKAEHGTGRNMAPFVEKEWGTEIYGLMKRIKSAFDPNHI
                     LNPGVIINDDKDVFIKNLKNIPDANPLIDKCIECGFCEVTCPSKELTFTPRQRIVAYR
                     RLTELADSRFDKKLRERWAGEMAYKFNETCATDGLCAIACPVNIDTGSLVKELRWKEN
                     GKFANRVASSMANNMASATSLIRGLLKIPHFIAKIIGYNPMEDITKGVYKMGDGLFPL
                     WTRYTPSGSRKIVRNIFPGHSADAPVVVYFPSCITRSMSGPSFGYAEKEDIPSKMLSL
                     LRKAGYTVIIPEGKDNLCCGMAFSSKGFRKQARQKESELNGALLKASRNGELPIVCDM
                     SPCLLHMRETLDSRLRLYDQVEFIHDFLLDKLTFEKQPVSIAIHTTCSSTKMHLEEKL
                     YTVAALCADKVIVSENVSCCGWAGDRGFFYPELNRSALASLKHDIGDATEGYSNSRTC
                     EIGLSINSGVTYKSLVYLVDRASERKFLS"
     gene            complement(11153..>11433)
                     /locus_tag="PLI30_02765"
     CDS             complement(11153..>11433)
                     /locus_tag="PLI30_02765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI78508.1"
                     /translation="SIKNSPLNYFASLWKKEAYPIGRSDGYHYLKVPVLVNSQNNGYI
                     QRIEQLEKYIFAWTDEKLTSWRSMLPQASELETFYEQLDETVSGFYGK"
BASE COUNT         2857 a         2728 c         2815 g         3033 t
ORIGIN      
        1 tacctggcct gccttctgga tgttgccgga gaaggagcag ctcaattggc ccctcgacgg
       61 agagattgat atcatggagt atgtgggtta tgatcccggg tggatacacg cttctgtgca
      121 tacaaaagcc tacaaccata caatcggaac ccaaaaaacc gccaggaaag aggttaaaac
      181 cgctgaaaca gcgttccata tttacgcagt ggaatggacc gcagactata tcaaaggatt
      241 cgtggatggt gtggagtatt tccggttcaa caacgatggt gccggaaaca aggagacctg
      301 gcctttcaat gtgcccttct acctgaagct gaacctggca tggggcggca attggggtgg
      361 cgcacagggt gtagatgagt cgaagctgcc tgctacctac gagatcgatt acgtacgggt
      421 gtatcaaaag aagtagaacc aagagacaaa acataaaaac aagagcttag cggattgctg
      481 aatgcttaaa aaaacataaa atgaacacga acaaaatttt ctttttttcc ttttttattc
      541 tgttactaac tgcctgtaac ggcggtaccg gaaaaaccgg gatgaccctg gataccggcg
      601 tggaaaaaaa gatcgacagt atcctgtcac aaatgacgct ggaagaaaaa gtggggcaga
      661 tggctcagtt caccatcgat ttgatcggaa aaggaggaaa tgcctatttc agtaacgaac
      721 cttttgaggt ggatccggcc atgctcgata ccgtgattgg gaaatacaag gtgggctcca
      781 tcctcaacac cagtaacaac cgggcgcgta ctaccgaagt atgggaaaat accatcagaa
      841 ccatacaaca aagggcattg caggagacgg gtattccggt aatctatggc atagatgcca
      901 tccacggtac tacatacacg gcaggaggag ttctcttccc gcaggccatc ggtatggcgg
      961 ctacatttaa tacaggactg atgaaagaag gatcgcgcat cactgcttat gaaacacggg
     1021 caagcaacat accctggacc ttcgcgccaa cgatggactt gggaagggat gcacgctggt
     1081 cccggcagtg ggagagctat ggcgaagatg cctacctcaa cgcacagatg gcggtggcct
     1141 ctacccttgg ctttcaggga gaggatcgca acaacgtagg tagccagcat gttgcggcct
     1201 gtttgaagca ctacatgggt tacggcgtac cggtgtctgg caaggaccgt actccgacgg
     1261 taatcccgga gaatgatttg cgggaaaagt atttcgagcc ttttcgtgcg tccattgagg
     1321 caggtgctct ttcggtgatg gtcaactcgg gaatgatcaa cggagtctct actcatgtcg
     1381 actaccggct gcttacgcaa tggctgaaag aggatctcga tttcgatgga gttattgtga
     1441 ctgactgggc ggacgtgcag aacctgctga gccgtgaccg tgtggccgct gattacaaag
     1501 aagccgtcaa aaaggcgatt aatgccggta tcgatatggt gatggaaccc tacaacctcg
     1561 ctttctgtcc caccctgata caactggttc aggaagatca ggttcccatg aaacggattg
     1621 atgacgcagt acgccgggtc ttgcgcttga aataccggct gggacttttt gaccgtccct
     1681 actggccgcg ggaagaatat cccgattttg ggaacagcgg gaacgaggag gcggcaaaag
     1741 cagcagcgga tgagtcgatt accttgctga aaaatgataa caatatccta cccttgcctc
     1801 ctgctgcacg aatcctagtt gcaggcccca atgctcactc catgcgtacg ctcaacggcg
     1861 gatggagcta ttcctggcaa ggagaaaaag tagaggagtt tgctcaggac tacaacacca
     1921 tctacgaagc gttgcagcag aaatttggag catccaacgt gagatatgaa cccggtgtta
     1981 cctataaaat ggacggacag tacttcgagg agaatgcacc cgaaatagca aaggctgtca
     2041 cagcagcggg aggggcggac tatatcgtgc tttgcctggg cgaaaactct tactgcgaaa
     2101 cgcccgggaa cctggatgag cttactctct ctgaaaatca aacagaattg gcattggcgt
     2161 tacaaaaaac aggtaagccc ctgattctgg tgctgaatga aggacgtcca cgactgatcc
     2221 gcaaaattga accgggtgct atggcggttg tgcaaaccta tctgcctgga aattttggag
     2281 gagatgcgct ggcggatatc ctgagcggcg atgtgaaccc gagtggaaaa ctgccttaca
     2341 cctatccgaa atacgaacag ggcctgatta cctacgacca taaacccagt cagaatattg
     2401 acggaaagat ggaaggtgcc tacgattacg gggcgcagac atcggtacag tatccgtttg
     2461 gctatggact gagctatact tctttcgatt atagcaatct ccgtgtgagt aacacacttt
     2521 ttacggcgga agatgtgttc tccgtatcag tagatgttac caacagcgga gcccgggaag
     2581 gaaaagaggc cgtgttgctc tttagcagtg atctggtagc atccctctct cccgatgtac
     2641 gccgcttgag agcgtttgag aaaatctcgc tgaagccggg agagacccgg acagtcactt
     2701 tcgaaatgaa aggcaatgag ctggcctttg tcaacgaaca ggggcattgg gtactggaag
     2761 aaggtgagtt tatgctgcag gctggaaacc agaccgccgg tattcgttgt acggcgacaa
     2821 aaatctggga aacgcccaac aaataagatg aactatttcg ttaagctaaa tgagaaaaac
     2881 taaatttgtt cggactttgc catagcgaga aaatgcctaa taaatagaaa tataaaattc
     2941 aaaacagttt cttggtgaat aacaggcgac agaattgatg taacgggagc acttttgata
     3001 gaggtgctcc cgtttacttg ttattgctcc cggataggtt ctacggcagg gcctatcctt
     3061 tattttttct tttcctttgc tttttatcct ccttaaactg aatcatgcgt gattctattc
     3121 cataagccgc gatatagttc tggattagct ccagaaactt cgcgccgaag cgttctgttt
     3181 taacttttcc gaaaccgtgg atcctcagta actccttttc cgtctccggt aggtaatccg
     3241 ccatttgtac caatgttttt actgaagcca caatatagac cggcagattt tcctctttgc
     3301 tgatgcggtt acggagctgt accagctcgc ttagtagttc ggggtggatt gatttcaact
     3361 gtatccccgt gctgtcacgg ctgtaggaag tgagggagaa aggaggttgc ctgaactggc
     3421 gtcgcgcatt gtaatacgac tctatgcaga agttatctga agtcgcagag atcaggtgcg
     3481 ccttcagggc tgccgctaca aatagggaaa cgatgtcctc gtcgtattcc tgcgcatttg
     3541 ccttgctatc cgtggttgcg gtcgattcct gcaatgtccg cagcaatgtt tctatttttt
     3601 cggtgaaata ggatgaggat gctcggaggc gttctgcaaa aaaaaccttg ttgacgggtg
     3661 tttgcccggt aatatgttgt aactgtatcc tgaatttgcc ggctacctgc tccaattccg
     3721 tcaactgatt acagacctca cttacgaaag aaacggttcc ctcgctgaaa gaagattcgt
     3781 tttcctgagt attggagtac caggaacgtg ctgcctggag aagcgggtcg aagtcataca
     3841 gttgcagtag caggttgatg ctgaactgct tctttgccaa ctgcaactct ccggcaacct
     3901 ggttttcttc tggtttagag gaggagaacc ggacaacctg ttcgtctacc cctatgctgt
     3961 aacggttaat cggacttttc aataccaggc tgtcgagcga ccggcaacga ctcagtgcca
     4021 catacacctg tcccggtgaa aaggctttcc ccgcgtcgat gatggcctta tcgaaagtaa
     4081 gtccctggct tttgtggatg gtgatggccc aggcgagccg caggggaatt tgtttgtatg
     4141 tgccgattat ttcctcttcc accgtattgg tctccgaatg ggtggtgtat cggatattct
     4201 cccagagtac aggagaaacg gtgatttctt ccgtgtcgtc cggacactgt acggtgacac
     4261 cctcttccag gatgtgtgtg accgtgccaa ttttaccgtt gaaataacgt cgcggtactt
     4321 ccgtatcgtt tttcacgaac attaccttgg cgccttcctt caggatgaga acccggtcca
     4381 cgggaaaagc attgtcgggg aattcccctt tcaccaccgc atggaacgaa tgagcggttg
     4441 tgttaatttt ttccagctcg gtactattga tcgcatcggc gctgaaatta tgcgtggtga
     4501 gcgtgatgta attttcttca ggcgtcgggt taaagtgcgg gtcgtaacgg ctttgcaaga
     4561 gctcgagccc ttccggtgaa agggcgtcgt tccgcacttc gttcaagaca tcgataaaaa
     4621 ggttgtcgga ctgccggaag atcttgtcga actccacata gaccggtggg tgttcctgga
     4681 tgacgcgact gtgaaaaaaa taaattcccc ggtagtagtc cgacaggatc cgccactcat
     4741 cggccttggc cacgggcgac agttggtata catccccgat aaaaactact tgcacaccgc
     4801 cgaagggttc agaacgcctg tggcgcacac tgcgaagcac cgtgtcgatg gcatccagca
     4861 catcagctcg caccatgctg acttcatcga tcaccagcac ttccagttca cgaatcacac
     4921 gacgccggaa gctgttcatt ttcatacggg caatcaacgc ttcacatccg gcaggtgttg
     4981 gcgtaaaagg ggtaaacggc aactgaaaaa aagaatggat ggttgcccca cccgcattaa
     5041 tggccgctac acccgttggt gctaccacgg ctacctgttt cgtggtttcc gtacgaaacc
     5101 ggtgcagaaa agtcgttttt cccgtgccgg cctttccggt gataaatata ttcctgccgg
     5161 tataggtgac aaattccttc gcacattcat atggagagag gtaggacatt ttttccatta
     5221 cctgtaaaaa cagggatatt tgttattgaa ccggtacccg atacccaaca tcaggtaatt
     5281 cggcgtttgg aagttctgaa gattatagcc gatatggata aacgaatttc tggtaacttc
     5341 cactttaagc gccaacatct ggtatagtcc ggccaggtcg ccacctccgt ggacgatatt
     5401 cgaaccgata cccacaccca ccgtaaagta aggcatcaca tactccgccc gtgcagaaat
     5461 acctaaagcc aattgtttgt taaccggtgg attatgaaac gtgtatccgg gatttttacc
     5521 accaggctca acaatatagt cttcggtata cacgtttgca ctgctgtcgt aaataccgtc
     5581 cagcgataaa ccggcacgca gcttgtaacc aaagttatac atggggttga tgttgaagcc
     5641 gcctaccgga tattcgtgcg gtgaggctat tcctttgcct tcaagataaa caccttttcg
     5701 ccgccacgaa ccgaacaaaa caatatcgta actcacatgg cggggaaaat ctgccactgc
     5761 tccccggtat ggggactgcc gggacattat actaggatta agcgtgtaca tcagccccag
     5821 cttgactcct gccgtattta atcctgcatt gggaaactgt gtgttcccgt tggaaaagtg
     5881 tgcgatgctg atacccccaa tcaggttgaa gtattgtgaa agtgtttggt tgagataaaa
     5941 tccggtgttc atatatgcgt ttattttcga gccgatgacg cggctatccg gatttgtttt
     6001 gggatggtaa ggtttccatc cgcccgataa cccgaagttc cactcgtaat gaagcgacaa
     6061 cttggaggtc atttgtgcta aacgagcacc ttgaaacaaa tacacggcaa ggggggctcc
     6121 caattgctga ggctcgccaa acgtaaaata tgcaactcca atcccttggt gcggtatacc
     6181 gtaaatgctc cctgcagggg agccgggctt aaattgaaag gagtacttca ggtgggtgga
     6241 aaacgaattc cgaatgggtt tggagcgggc attctcgcct ttcagaaaat tatgggtagg
     6301 aataatgtat cccggccggg cttcgaattc caatctgttc cggaacctat ccggttggag
     6361 cacagaatct gaacgtgttt gagcggacga gggtttcgtg gcgaaaaaca ggaaaacagt
     6421 agcaaaaatt aataatttgt atgtgatttg tttgttcata aaaatctaat ccccgattcc
     6481 caactccgca atccgtttac atcctttccg gcacttcaat cccaaacaat accattccct
     6541 ttttgatggt tatggctacg ttcttcgaaa gcagcaggcg gaacgcacgc agggcttcgt
     6601 ccttttcacg cagcatgggg aaatcgtgat agaactggtt gaattctttg gccagatcgt
     6661 acacgtaatt tccgattagc gagacgttgt attcgtctcc ggcctgtttg acgatatccg
     6721 gaaattcagc cagcaactgc accaaccctt cttctttttc agaaagaaca atgtctgcct
     6781 gcattgattc aggaacagga atcccttgtt cctcggcttt gcgcaaaact gatttgatgc
     6841 gggcgtgggt gtattggata aacggaccgg tattcccgtt aaaatcaatg gattctttcg
     6901 gattgaaggt catgtttttt ttcggatcta ctttcagaat gaaatatttc agggccccca
     6961 ttccaatcat ggttacaaca cgatcggctt cctctggtgt tacaccgtcc agctttccca
     7021 gttcttccga agtttcgcgg gcggtctgaa tcatctcggc catcaaatca tcggcatcca
     7081 ctacggtccc ttcacgcgat ttcatcttgc cttcgggcag ttccaccatc ccgtaagaga
     7141 aatggaccaa tccttttccg aattcaaaac ccagcttatc gagcagtatg gagagcacct
     7201 ggaaatggta gttttgttca tttccaacca cgtaaatcat ggtgtctatg ggatagtcat
     7261 cgaaccggag cttcgctgtg ccgatatctt gcgtcatgta cactgaagtt ccgtcggagc
     7321 gaagaaggat tttttcatca agcccttcat gggttaaatc ggcccatacc gacccgtctt
     7381 cacgctggta gaaaacaccc tctttcaatc ctcgcagcac ctcgtctttt ccagccaggt
     7441 aggtttgcga ctcataatag atcttgtcga acgagacccc cagcttttcg tacgtctcat
     7501 cgaaaccggc atacacccat ccgttcatca ttttccataa attcacagtt tgggcatcgt
     7561 ttgcttccca ttgacgaagc atctcgcgcg cttcctccat caaaaccgat tgggcttcgg
     7621 cttgctccct tgatagtccg gcagcctgca ggtcggctaa ttcggctttg tattttttat
     7681 cgaaaagcac ataatacttg ccgatgaggt ggtcgccttt cttgcccgat gatgcgggtg
     7741 tttccccatc tccccatttc tgccaggcca gcatcgattt gcagatatgg atacctcggt
     7801 cgttcacgat attggttttt accacccgtt taccgttggc tttgagtatt tcacagagcg
     7861 aatgtcccag gagattgtta cggatgtgtc ccagatgaag cggtttgttg gtgttgggcg
     7921 acgaatattc caccataaac aagggtgcgt cattagcaac aggtgtgaag ccgaattcgg
     7981 gatttttatc gatgttctcg agcagatcaa gccatacttt cgaggcaatc gtcaggttta
     8041 gaaaaccttt tatcacgtta aactccgaaa caacgggtga tttatcggcc aggtacatac
     8101 cgatttcctg tgccgtttgt tcgggattct tcttcgaaat ctttaacaaa ggaaaggtta
     8161 cgagggtgaa atgtccttta aattctttct ttgttttctg aagtgtgatt tgcgatgcat
     8221 ttacatctac gctgtacaat tcttttattg cgcttataat tgcagtctga agtatattct
     8281 cgatgttcat aatgtgaatt tgctgattga gtgtgcaaag atacaaacaa tctacgaaag
     8341 aaacttcctc tccgaagccc tatccaccag atataccagc gatttatacg ttacaccgct
     8401 gtttatcgac aagccgattt cgcaggtgcg actgttggaa tacccttcgg tagcatcgcc
     8461 gatatcgtgc ttaagggatg cgagcgccga acggttcaac tcgggataga aaaagccgcg
     8521 atcgcctgcc catccgcaac acgaaacgtt ttcggagacg atgactttgt cggcacaaag
     8581 agccgcgaca gtatatagtt tttcttcgag gtgcattttc gttgaactgc atgtggtatg
     8641 aatggcgatg gacacaggtt gcttttcgaa ggtcagtttg tctaacagaa aatcatgaat
     8701 aaactctacc tggtcgtaca gtctcaggcg ggagtcgagc gtttcacgca tgtgcagcag
     8761 gcagggactc atgtcgcaca cgataggcaa ttcgccgttc cggctcgctt tgagcaatgc
     8821 accgttcaac tccgactctt tctgccgcgc ttgcttccga aaccctttac tgctgaatgc
     8881 cattccgcag cacaggttgt cctttccttc agggattata acggtataac ctgcttttcg
     8941 caacagcgaa agcatttttg aagggatatc ctctttttct gcgtagccga aagaagggcc
     9001 gctcatggag cgcgtgatgc acgatgggaa ataaaccact acgggcgcat cggcactgtg
     9061 gcccggaaaa atgttacgaa caattttccg gctccccgac ggcgtatatc ttgtccataa
     9121 gggaaagagg ccgtcgccca ttttgtatac ccctttcgtt atatcttcca ttgggttgta
     9181 acctataatt tttgcgatga aatgggggat ttttaacaaa ccacgtatca gtgatgtggc
     9241 cgatgccata ttattagcca tactgcttgc aactctgttc gcgaattttc cgttttcttt
     9301 ccagcgaagt tctttcacca acgaccccgt atcgatgttt acagggcagg caatggcgca
     9361 aagtccgtcg gtagcgcaag tttcgttaaa cttatatgcc atttctcctg cccatctttc
     9421 gcgcaacttt ttatcgaaac gactatcggc taattccgtt aacctgcgat acgctacaat
     9481 acgctgacgt ggtgtaaagg ttaactcttt tgacggacaa gttacttcgc aaaacccgca
     9541 ttcgatgcat ttgtcaatca atggattggc atcggggatg ttttttaggt tcttgatgaa
     9601 aacgtcttta tcgtcgttga taattactcc cggattcaat atgtgatttg ggtcgaaagc
     9661 cgattttatg cgtttcatta acccataaat ctccgtgccc cattcctttt ccacgaaggg
     9721 agccatgttt cgccccgttc cgtgctcggc tttcaggctg ccgttatgtt tcacagccac
     9781 cagattggcc agttcatgca taaaacgggc gtacttgtca acaccgtccg gtgaatttat
     9841 gtctggaaaa actgtaaaat gaacgttccc atccagcaag tgtccccaca ttacggcatc
     9901 ggcataaccg gtccgcacca acagttcacg cacgtcggtc agcgcgtcgc ctaaaatttc
     9961 accgctaaag gcaatgtctt caataatgct tgccgtgtgt ggtgggcgtg atgccgctgc
    10021 cgaagtaaac aacccattac gtacgttcca gtaaaggtta tacagatgaa tgtcggttgt
    10081 aaaccgtatg ggataaagtg tttgaatgtg cgccagtttt gcttcaatgt cacgcatctg
    10141 gacaagcagc gtttcttcgt cgtcggcaga ggtgtctata agcaatgccg ctgccccttc
    10201 cggaagtgac ttcagttcgg caggcatccc ttcctgattt tccacagcac gtaaagcgtt
    10261 cctgtccatc aattcggcgg cgcttaccga acacgaacgc aacggtagaa tggtttcgca
    10321 cacatcgcgc aagttatgga aatagagcat ggctgtagct ttgagaggcg catcatgtac
    10381 ggtttcgaac gtggcttgcg atacaaaccc cagtgtacct tctgagccga tcatcaggtg
    10441 ttgcagaata tctacagggt cggagaaatc gattaacgaa tttacgccgt atccgcatgt
    10501 gtttttgagt tggaacttct gttgtatccg gtttttaacc ccttcgttcc cagaagcctc
    10561 gttgtgcaag cgttcaatct gagcaataaa ttcgggatga gatgcgataa acgactgaca
    10621 actggttgta tcggcggtat cgagcaatga gccgtcggca aaaataatcc gcatcgattt
    10681 tacggtatgg taactgttgt gcttaatgcc gtaactcgaa ccgctggcgt tgttggcaat
    10741 aatgcctcca attttcgccg agttgatgga tgctggtttg gggcctaatt tgcgacggta
    10801 gcgctgcaac atacggttgg ctgccgttcc ggtaagaccc gcgccgaaag tagccgtttt
    10861 cccgttttcc gttatgactg aaaactcaaa tccctgcccg atttccatca ggatagaatc
    10921 ggaaacggtt tgtccgctca aacttgttcc cgcggctttg aaagttacgg ggacgttttc
    10981 tttactgcag aactgcagta atcggataac ttcgtcttcc gtgttgactt ttaccactgc
    11041 cttgggaaca agccggtata atccggcatc ggtgcctttg acgaggcgcg agagttcgtc
    11101 ggtgaaaacc tgttctttgg gaaaaagttt tgttaagcga gaatgtagtt gtttattttc
    11161 cataaaatcc actcactgtt tcatccaatt gttcataaaa cgtttcaagt tcagatgctt
    11221 gcggcagcat gcttctccac gaggtcaatt tctcgtccgt ccatgcaaaa atatattttt
    11281 ccaattgttc gatacgttga atatagccgt tgttttggga gttgacgagc acgggcactt
    11341 ttaaatagtg atacccgtcg gaacgaccga tggggtaagc ctctttcttc cacagggagg
    11401 cgaagtaatt gagtgggctg tttttaatgg att
//
LOCUS       DAOSDR010000041        11387 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrimonas sp. isolate AMR_MDS_1826
            NODE_2185_length_11387_cov_4.521444, whole genome shotgun sequence.
ACCESSION   DAOSDR010000041 DAOSDR010000000
VERSION     DAOSDR010000041.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863355
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrimonas sp. (wastewater metagenome)
  ORGANISM  Petrimonas sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Dysgonomonadaceae; Petrimonas.
REFERENCE   1  (bases 1 to 11387)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11387)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:10:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,720
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 35
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11387
                     /organism="Petrimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2185_length_11387_cov_4.521444"
                     /isolate="AMR_MDS_1826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2023866"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            158..793
                     /locus_tag="PLI30_02770"
     CDS             158..793
                     /locus_tag="PLI30_02770"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009318275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HOI78509.1"
                     /translation="MDNRLEEYILSHIDPEPELLKEIDREVHVKLLHASMMSGHLQGR
                     LLKMLTRMIRPERVLEIGTFVGYSTLCFAEGLEENGEVHTIEIDDELEDFIRSNFAKS
                     ELANKIKLHIGDATQIIPEFEDESFGLAFIDADKELYWKYFEATLPKIRKGGFILVDN
                     TLWYGKVVEKVESSDRATQGILNFNEKLANDDRVEKVILPVRDGITVVRKK"
     gene            800..1132
                     /gene="rbfA"
                     /locus_tag="PLI30_02775"
     CDS             800..1132
                     /gene="rbfA"
                     /locus_tag="PLI30_02775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010535697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HOI78510.1"
                     /translation="METNRQQKVNRLIQKELGEIFLVETKKMPGVLVSVTHVRVTPDL
                     GIAHSYLSIFPSEKGSEMVRNINDNVKSIRYNLGKRVGKQLRVVPELVFYLDDSLDYI
                     ENIDKLLK"
     gene            1134..2366
                     /locus_tag="PLI30_02780"
     CDS             1134..2366
                     /locus_tag="PLI30_02780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008778805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOI78511.1"
                     /translation="MNLSLFIARRYLFSKKSHNAINVISLISVCGIAIATMAMVCVLS
                     VFNGFGGIVEGMFSAFDPDLKITAKAGKVFDYHTPDFDKALRIDGIQMISESLEENAL
                     YKFEDRQVPVLVKGVSEEFRLMTDMDKLMVDGSFRLTEDVVNYTTLGTGLAITLGARA
                     GFINPIEIYAPKRDVNVNLANPSAAFTREDIQIGGVFSLNQPDVDDQVAIIPIRLARE
                     LFRYDNEVSSLDIKLHPDASVKKVKSEIEKILGDDFLVENRFEQQIESFRMLQIEKWV
                     TFLILAFILLIAVFNVVGSLSMLIVEKTIDIKSLKNMGANNNLISRIFLYEGWLITFF
                     GIVSGIVAGLTLCLLQQHFGLLRLSNVPGAYVVDAYPVIVRFWDIVTVFVVVSIISLL
                     TVFYPINNLKKKLKFAEV"
     gene            2400..3716
                     /locus_tag="PLI30_02785"
     CDS             2400..3716
                     /locus_tag="PLI30_02785"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006798422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose/GDP-mannose dehydrogenase family
                     protein"
                     /protein_id="HOI78512.1"
                     /translation="MKIAVVGTGYVGLVSGACFSEMGVDVTCVDIDEAKIEGLKQGII
                     PIYEPGLKNIVVRNHDAGRLHFTTDLPSVLNDMDMVFIAVGTPSDEDGNAEMKYVWKV
                     TETIGDHIDKPLLIVTKSTVPVGTSHRIKERVQKRLDRRGLSNLEFDVASNPEFLKEG
                     AAVDDFMSPDRVVVGVESERAKELMTRLYRPFLLNNFRVIFMDILSSEMTKYASNAML
                     ATRISFMNDIANLCERVGADVNMVRRGIGSDTRIGKNFLYPGCGYGGSCFPKDVKAII
                     KVAENAGYEMSVLKAVEQVNENQKKILFQKFSEFFNGVVEGKTVAVWGLSFKPETDDV
                     RDAPALQLIDSLLESNVNVRAYDPAAMDEAKKRLNDKIYYAKDIYDAANNADALIVPT
                     EWKEFRLPNWEILKKIMRTHLVIDGRNIYNKEDLFSYGFGYRGIGL"
     gene            3740..4366
                     /locus_tag="PLI30_02790"
     CDS             3740..4366
                     /locus_tag="PLI30_02790"
                     /inference="COORDINATES: protein motif:HMM:NF013077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HOI78513.1"
                     /translation="MDLIEALNWRYATKRMTGEKIAESDLNIILEAIRLTPTAYGLQP
                     FKVVVIRNEDLIGEIYRKSCPQIVIRQCSHLLVFKARKKLDSEYLEGYLKEMKRQRNS
                     TDEYIDGYRSKILRVIDDPQINKFSWMIRQTYIALGYATVAAAASGIDATPIEGFDAQ
                     SLNEVLRLDTEKEEAVVMLALGYRDEKEDKLIYLPKIRKPMDLLVEKI"
     gene            complement(4377..5324)
                     /gene="gldN"
                     /locus_tag="PLI30_02795"
     CDS             complement(4377..5324)
                     /gene="gldN"
                     /locus_tag="PLI30_02795"
                     /inference="COORDINATES: protein motif:HMM:TIGR03523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility protein GldN"
                     /protein_id="HOI78514.1"
                     /translation="MKFKPIFISFFIFCCIGNCFSQETARERLQRRNQGQSNTNSTVE
                     LTSRATQMNRTQSTDINDAKWLREIYRYLDLSKEGNAPLYYPVLPEQGRMNLFTLIFN
                     LLSENKITAYEYLDGREVFTDEYKIDFKEFLDRFGIFHETQNGNIVVSEVDIPSHEVL
                     GYYLKEAYYFETGTSKYGVKTVALCPIIYRQADYESQTVRYPLFWIVYDELRPYTLRM
                     PIMTSSLNNTMTGTIDDFFRMKHYDGEIYKATNPRNLAISQYTSTPEEMKAEQERVEQ
                     QLRDFEERLWKGDTDTTRVLPYRQKQRAGGSTTMRNRRY"
     gene            complement(5372..6928)
                     /gene="gldM"
                     /locus_tag="PLI30_02800"
     CDS             complement(5372..6928)
                     /gene="gldM"
                     /locus_tag="PLI30_02800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007212033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility protein GldM"
                     /protein_id="HOI78515.1"
                     /translation="MIINNPNSPRQKMINLMYLVFIAMLALNVSAEVLTGFDIVGDSL
                     SNSSENMHTRNRLIMDELEGYNSQNREKAGEWYDKGVQVKQMSDSLVSYIEDLKIRIV
                     KESDGKKGDINNIRNKDNLDAASSVLLSPPARQGHRLRTAVDHFRQTVTGLIQDDNRR
                     KIIENNLSTTPSERNDSHKNWEESLFEKMPVSAAVAILSKIQNDVRLSEGEALGSLLN
                     SIDVSDFRVNELNAYVIPESKVIIQGGTYNARVILSAEDSTLSPDIIVNGQSLDPSAK
                     GFFSTASSAVGTFPVEGYIETRGSDGSTVRRSFSDNYTVIEPAATIAPTLMNVLYAGI
                     GNEISISVPGIAPQDVSGTMTNGSLVRKGNLWEAKPVAAGRDATVSISARTGSQVRQL
                     ASKNFRVRSLPDPSPYIEYADANGNPVVFKGGNLAKAVLVNTQGIKAAIDDGILNIPF
                     RVTGFRTLFFDSMGNAIPEVSEGSRFSERQKEQIRRLQRGKYFYISGVKAIGPDGLER
                     EIAVIEVRVN"
     gene            complement(6925..7533)
                     /gene="gldL"
                     /locus_tag="PLI30_02805"
     CDS             complement(6925..7533)
                     /gene="gldL"
                     /locus_tag="PLI30_02805"
                     /inference="COORDINATES: protein motif:HMM:TIGR03513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility protein GldL"
                     /protein_id="HOI78516.1"
                     /translation="MNKIIRKIENSLQTDNGQRFLQYAYSFGAAIVIFGAMVKVLHWW
                     GIWGNIIFGTGMAIECIVFILYGLDRPPKAVPAEKEAPAIQAAQDLPVTSRYPKEQPG
                     EVSENVMKFEQEYVQQMKALNRNISGLNTIYEIHLKGISGQLDTLDQINKGLIRIKEM
                     YEGSVPDSSIFSRETEKMTRQIQELNQIYGRLIHAMTNTQPK"
     gene            complement(7555..8967)
                     /locus_tag="PLI30_02810"
     CDS             complement(7555..8967)
                     /locus_tag="PLI30_02810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005633367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HOI78517.1"
                     /translation="MKKLIVPILILIVLASCGRRGIGISTGGELTGVPVGKVWNEPTP
                     YNMVLVTRGSYRMGPGEIDSLWGMTVPTRGVSVDNFWMDEAEITNSQYKQFVFWVRDS
                     IIRERLADPAYAGDDFFRITEDEYGEPVQPRLNWSIPIPWTRNTEEEEAAINSVYITH
                     PITKKKMLDARQMNFRYEWFDAAEAAKRQNRLDPQERVLNTDIAANPDEVIMISKDTA
                     YIDEEGRIVNETITRPLNSLYDFVHTRIVNIYPDTTCWVNDFSNANNEYYMRNYFSHP
                     GYAHHPVVGVSWEAATAFCEWRTLFLRRGLQRRDVKVEKYRLPTEAEWEMAARSGKTE
                     NRYPWGPTAAQAEEDCYQANFKPGEGAYAADRNLIPAKTKSYRPNSFGLYDMAGNVAE
                     WTSTAYTESGNQLMSDMNPEYRYNAAAEDPYPLKKKVVKGGSWKDISTFIRSDMRDSE
                     YQNHGRSYIGFRCVRTPIGN"
     gene            complement(9030..9986)
                     /locus_tag="PLI30_02815"
     CDS             complement(9030..9986)
                     /locus_tag="PLI30_02815"
                     /inference="COORDINATES: protein motif:HMM:TIGR03519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorP/SprF family type IX secretion system
                     membrane protein"
                     /protein_id="HOI78518.1"
                     /translation="MSAFFLFCIAQMQAQWDVPFNQFWTAKTYYNPSFAGETGKIQIS
                     GIYNYRWTNIKDAPKHLFLSMDMPVEFLGLQHGIGIQTLSNVTGNKQNTLFAAQYTFN
                     QKFGKGILRIGVQAGMLNLNFDAASLQLTTDSAKNNRKIIRAQPTDRKSLDLNAGISW
                     TTRNFHVGLAAMHLNQPSFYPLRSPGPPENAKADSIHSRIPLSYNFMAGCNINVFHPL
                     VEIQPMALLMTSLTDTRLHTAVRMVYGKKYSAGVSRNGKEGCSLFAGAIIKNIEMGYA
                     YSMYTSGIGKGSGGSHEVSFRYRFPIDLFRREPMPYKSIRLL"
     gene            complement(10081..11169)
                     /locus_tag="PLI30_02820"
     CDS             complement(10081..11169)
                     /locus_tag="PLI30_02820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019538525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HOI78519.1"
                     /translation="MENKYIISGIQQVGIGVENLHEAWKYYIDVFHMDIRILEDNKVA
                     ELMLPYTGGVPQRRHAVIAVNLQGGGGFEVWQYAGRKPKRLGFELNMGDLGVLACKIK
                     SRDVAKTFGEFSRDPKVNIVTKLSQDIDGKQTFYVKDPYGNLFQIVQDEYIFKNEGRS
                     TGGAVGALIGVTDIEKAMVVYKDILGYDRVIADETGVFADFEGLPSGSQRFHRRLLTH
                     SQPRKGSFSELFGPSYIELVQPLERSPRKLYEGRFWGDPGFIQICFDIRNMDALGKHC
                     AELGHPFTVDTTQKFKEGASFDMGDAAGQFAYIEDPDGTLIEFVETHKVPVMKKLGLS
                     INLKNRNPEKPLPKWMLKMLRFGRVKGD"
     gene            complement(11195..>11387)
                     /locus_tag="PLI30_02825"
     CDS             complement(11195..>11387)
                     /locus_tag="PLI30_02825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019538524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="short-chain dehydrogenase"
                     /protein_id="HOI78520.1"
                     /translation="RHAQMDAGQAEGIPAEKAARKIVKAIWKQKPEVLVGGKELLMVY
                     IKRFFPGLARKIVRKIRTT"
BASE COUNT         2837 a         2546 c         2814 g         3190 t
ORIGIN      
        1 gattgagaat aagatgatca agcctgagga tcgcgtggcg tacatgggcg gcagtttcgg
       61 tgaaaccggt ggaaccactt atcttgaaat agcagaagcg tggagaattt tggaagcgaa
      121 agacaaatat aatttgccgg attatacgca ataaaagatg gacaatagac tggaagagta
      181 tatattatcg catatcgatc cggaaccgga attactgaag gaaattgaca gggaggtcca
      241 cgttaagctg ctccatgcga gcatgatgtc tggccatttg caaggccggc ttctgaagat
      301 gctaacccgg atgatccgtc cggaacgggt gcttgaaatc gggacttttg tagggtattc
      361 caccttgtgt tttgctgaag ggctggaaga gaatggcgag gtacatacga tagaaatcga
      421 tgatgaactg gaagatttta ttcgttcgaa ttttgcgaag tctgaactag caaacaagat
      481 aaaactgcat atcggagatg ctacacaaat cattccggaa tttgaagacg aaagttttgg
      541 ccttgccttt atcgatgccg acaaggagtt gtattggaaa tatttcgagg ctacgcttcc
      601 gaaaatcaga aaaggcggtt tcattcttgt tgacaatacg ctctggtacg gaaaagttgt
      661 agaaaaggtg gagagcagtg accgggcgac ccagggtatt ttgaatttca acgaaaaact
      721 ggctaacgac gacagggtag aaaaggtaat cctccccgta cgtgacggga ttacggttgt
      781 tcgaaaaaaa tagagaagaa tggaaacaaa cagacaacaa aaagtgaatc ggctgattca
      841 gaaagagctc ggagagatct ttctggtgga aaccaaaaag atgccgggcg tactggtatc
      901 ggttacgcat gtacgggtaa ctcccgacct gggaattgcc cactcgtacc tgagcatatt
      961 cccttcggaa aaaggaagcg aaatggtgcg gaacataaac gataacgtga aatcgatccg
     1021 atacaatttg ggaaaacgag tcggaaaaca gttacgtgtt gtacctgaac tggtttttta
     1081 cctggacgac tcgctcgact acatcgaaaa tattgataaa ttgttgaaat aacgtgaatc
     1141 tttcactatt catcgctcgc cgatatcttt tctctaagaa atcacacaac gccattaacg
     1201 tcatttcgct tatttcggtt tgtggaattg ctattgcaac gatggcaatg gtttgtgttt
     1261 tgtcggtctt caacggattt ggcggaattg tggaagggat gttcagcgct ttcgacccgg
     1321 acctgaaaat tacggccaaa gccggaaagg tatttgatta tcacacgccc gactttgata
     1381 aagctttacg gattgatggc attcaaatga tatcggagtc gctggaagag aatgcgttgt
     1441 ataagtttga ggaccgccag gtgccggtcc tcgtgaaagg tgtatcggaa gagttccggc
     1501 tgatgaccga tatggataaa ctgatggtag acggcagctt ccgccttacc gaagacgtcg
     1561 tgaattacac taccctggga acaggcctgg ccatcacgct gggtgctcgg gccggattca
     1621 taaacccgat tgaaatttac gccccgaagc gcgacgtaaa cgttaacttg gcaaacccct
     1681 cggcagcatt cacgcgcgaa gacatacaga taggtggtgt tttcagcctc aatcaacccg
     1741 atgtggatga tcaggtggcc attattccca tccgtttggc gcgtgaactg ttcaggtatg
     1801 ataatgaagt ctcctccctc gatatcaaac ttcatcccga tgcgtcagtg aagaaggtaa
     1861 aatctgaaat tgaaaagata ttgggcgacg attttctggt ggaaaaccgg ttcgagcagc
     1921 agattgagtc tttcaggatg ttgcagattg aaaaatgggt gacgttcctt attctggctt
     1981 tcatcctttt aattgctgtt tttaatgtgg ttggatcact ttcgatgttg attgtggaaa
     2041 agacgataga tatcaaaagc ttaaaaaata tgggagctaa caacaacctt atttcccgca
     2101 ttttcctgta cgagggctgg ttaatcactt tttttggaat cgtttcagga attgttgccg
     2161 gattgacctt gtgtttgttg caacaacatt tcggactgct ccgcctgagt aatgttcccg
     2221 gagcttatgt ggtggatgct tatcctgtta ttgtgcggtt ctgggatatt gtaaccgtct
     2281 ttgtagtagt tagcatcatt tcgctgctta ctgtatttta tcccatcaat aacttgaaga
     2341 agaaattgaa atttgcggag gtataagaac tatacttttc gctcaaaact atattaatta
     2401 tgaagattgc agtagttgga acaggatatg taggattggt ttcgggcgca tgcttttccg
     2461 aaatgggagt ggatgtaact tgtgttgaca ttgacgaagc aaaaatcgaa ggattgaagc
     2521 aaggtattat cccgatttac gaacccgggt tgaaaaatat tgtggttcgc aatcacgatg
     2581 ccggtaggtt gcactttact accgatttac cgtcggtttt gaacgatatg gatatggttt
     2641 ttatcgctgt cggaaccccc tcggacgaag acggaaatgc tgagatgaag tacgtctgga
     2701 aggttacaga aaccattggc gaccacattg ataaaccgct gctgatcgtt accaaaagta
     2761 ctgtcccggt gggtacttca caccggatca aggaacgggt gcagaaacga ttggatagac
     2821 gcggcctcag caacctggaa tttgatgtag cctccaatcc ggaattcctc aaggaaggcg
     2881 ctgctgttga tgattttatg agtcccgacc gtgtagtggt gggtgtggag tcggaacgtg
     2941 ccaaggagtt gatgacccgg ctttaccgcc cgtttctgct taacaatttc cgtgtaattt
     3001 tcatggatat tctttcttcg gagatgacca aatatgcctc taacgctatg ctcgcaacac
     3061 ggatcagctt tatgaacgat attgccaacc tgtgcgaacg ggtaggtgca gatgtgaata
     3121 tggtgcggcg ggggatcggc agtgacacgc gtatcggaaa gaactttctt tacccgggtt
     3181 gcggttacgg cggaagttgt tttcccaaag atgtaaaagc gatcatcaag gtggccgaaa
     3241 atgcagggta tgagatgagt gtgcttaagg cagtagagca ggtaaatgaa aatcaaaaga
     3301 agattctttt ccaaaaattc agtgagtttt tcaacggtgt agtagagggc aaaaccgttg
     3361 ctgtctgggg gttgtctttt aaacccgaaa ctgacgatgt gcgggatgca cctgctctgc
     3421 aactgataga cagtttgctg gaatcaaatg taaatgttcg cgcatatgat ccggcagcta
     3481 tggatgaagc caaaaaacgg ttgaacgata aaatatacta cgctaaagat atttacgacg
     3541 ctgcgaacaa tgctgatgct ctcattgtcc ctacggaatg gaaagagttt cgtcttccca
     3601 actgggagat tctgaaaaaa attatgcgta cgcacctggt tatcgacggg cggaacattt
     3661 acaacaagga agacctcttt tcctacggat tcggatacag gggaataggt ttataacacg
     3721 aagttcaggt tatcaaaaaa tggatttaat agaagcgctt aactggcgtt atgcgacaaa
     3781 acggatgaca ggggaaaaga tcgcggaaag cgacctcaac atcatcttgg aagccattcg
     3841 tttaacgccc acagcatatg gtttacagcc ttttaaagtg gttgttatcc gaaatgaaga
     3901 ccttatcggt gagatttaca ggaagtcttg cccgcaaatt gtgatcaggc aatgctctca
     3961 cctgcttgtt tttaaagcaa ggaaaaagct cgactccgaa tacctggagg gttatttgaa
     4021 agagatgaag cgccagagaa actccacgga tgaatacatc gatggttatc gttccaagat
     4081 tttaagggtg attgacgatc cgcagatcaa caaattcagc tggatgatca ggcaaacgta
     4141 tatcgcgcta gggtacgcca ctgttgcagc ggcagcgtcg ggaatcgacg ctactccaat
     4201 tgaagggttt gatgcgcagt cgctcaatga ggtccttcgt ctcgatacgg agaaagagga
     4261 ggctgtagtg atgctggcgt tgggttaccg ggacgagaaa gaggataaac tgatttattt
     4321 gccgaaaatc agaaaaccga tggatctact ggtggagaag atttagctat aaaaggttaa
     4381 taccttctgt ttcgcatggt agttgaacct ccagccctct gtttctgacg atagggcaga
     4441 acccgtgttg tatcggtgtc ccctttccac aatctctctt caaaatccct gagctgttgc
     4501 tcaactcgtt cttgttccgc cttcatttcc tcgggagtgg aagtgtattg tgaaatggcc
     4561 aggttgcgtg ggttggtggc cttgtaaatt tcaccgtcgt agtgtttcat gcggaaaaaa
     4621 tcatcgatcg ttcccgtcat ggtgttgttg aggcttgaag tcatgatggg catacgcaga
     4681 gtataggggc ggagttcatc atacacgatc cagaagagcg gatagcggac ggtttgggat
     4741 tcgtagtcgg cctgacgata gataatggga caaagggcaa ctgttttaac cccgtatttc
     4801 gatgtcccgg tttcaaaata ataggcttct ttcaggtaat accccagtac ttcgtgactg
     4861 ggaatatcca cctcgctgac gacgatgttt ccgttttgag tctcgtggaa aattccgaac
     4921 ctgtcgagaa attccttgaa atcgatttta tattcatccg tgaagacctc cctgccgtcc
     4981 aggtactcgt aagccgtaat tttgttttcg gagagcaggt tgaaaatcag ggtgaacagg
     5041 ttcatccgac cttgttccgg caataccgga tagtagagcg gcgcgttgcc ttcctttgac
     5101 agatcgagat aacggtaaat ttcacgcaac catttggcat cgttgatatc cgtggattgt
     5161 gtacggttca tctgtgtggc gcggctggtt aattccacgg tgctgtttgt attggattgg
     5221 ccttgattcc ggcgttgcag cctttcccgc gcagtctcct gggagaaaca gtttccgata
     5281 caacagaaaa taaagaacga aataaatatt ggcttaaatt tcattgtcgt ataatttttt
     5341 aaatttccag tccgggactt caatgcgtaa atcagttcac cctcacctca atcactgcta
     5401 tttccctttc caacccatcg gggccgatgg ctttcacgcc cgaaatatag aagtatttgc
     5461 cgcgttgtaa ccggcggatt tgttcttttt gcctttccga aaaccgactt ccttcagaca
     5521 cttcgggaat agcatttccc atggagtcga aaaacagggt gcgaaagccg gttacccgga
     5581 agggaatatt taaaatgccg tcgtcaatgg cggcttttat cccctgagta tttaccagca
     5641 cggctttggc caggttgccg cctttaaaaa caaccgggtt cccgttggca tcagcatatt
     5701 cgatataggg gctggggtcg ggtagagagc ggacacgaaa attttttgac gccagttgcc
     5761 gtacctgact gcccgttctc gcggatatcg agacggtggc atcccggccg gcggcaacag
     5821 gtttggcttc ccataagttg ccttttcgga cgagtgaacc gttggtcatg gttcccgaga
     5881 cgtcttgcgg tgcaattccc ggtacggaga tactgatctc gttcccgatc cccgcataaa
     5941 ggacgttcat cagcgttggt gcaatggtgg ctgcgggttc tataacggta tagttgtccg
     6001 aaaagcttct gcgtaccgta ctgccatcgc ttcccctcgt ttcgatatag ccttccaccg
     6061 gaaaagttcc cacagcggag cttgctgtgg agaagaaccc ttttgccgag gggtcgagag
     6121 attggccgtt tacgattatg tcgggagata gcgtggagtc ttccgccgaa aggatgaccc
     6181 gggcattgta agtaccacct tgaatgataa cttttgattc ggggatgacg tatgcattga
     6241 gttcatttac cctgaaatcg cttacgtcga tgctgttgag caggctgccc aacgcttcgc
     6301 cttccgacag gcggacatcg ttctggattt tggaaaggat agcaacggct gcagacacgg
     6361 gcattttttc aaagagcgac tcctcccagt ttttatggga gtcgttcctc tcagaagggg
     6421 tcgtgctgag gttgttttca atgatttttc gtcggttgtc gtcctggata agaccggtta
     6481 cggtttgtcg gaagtggtca acagctgtgc gcaaccggtg tccttgtcgg gccggagggg
     6541 ataacaggac agaagaggct gcatccaggt tgtctttatt ccggatattg tttatgtctc
     6601 cttttttgcc gtccgactcc tttacaattc ttattttcag gtcttctata tagctcacta
     6661 gcgagtcgct catttgtttc acttgcaccc ctttgtcgta ccattcaccg gccttttccc
     6721 tgttttgaga gttgtacccc tcaagctcat ccataatcaa ccggttcctg gtgtgcatat
     6781 tttccgatga attgctcagg ctgtcaccaa caatatcgaa tccggtgagc acctctgccg
     6841 acacgttaag cgccagcatg gcgataaaca ccaggtacat caggtttatc attttctggc
     6901 ggggagagtt gggattattg ataatcattt gggctgcgtg tttgtcattg catgaatcaa
     6961 gcgtccgtaa atttggttta gttcctggat ttgccgggtc attttctcgg tttccctgct
     7021 gaaaatagaa ctgtcaggta cgctcccttc atacatctcc ttgattctga ttaagccttt
     7081 attgatctgg tcgagcgtat cgagttgccc gctgatgcct ttcagatgta tttcgtaaat
     7141 ggtgttcagt cccgatatat tccggttgag tgctttcatt tgctgaacat attcttgttc
     7201 aaacttcatc acgttttctg aaacctctcc gggttgctcc ttcggatacc gggaagtgac
     7261 ggggagatcc tgtgctgcct ggattgccgg agcttctttt tcagccggaa cagcctttgg
     7321 tggacggtct aacccgtaga gaataaaaac aatacactct atggccattc ctgttccgaa
     7381 aatgatattt ccccaaatac cccaccaatg caataccttg accatagccc caaaaataac
     7441 gatggctgcg ccaaagctat aggcgtattg cagaaaccgt tgtccgttat cggtttgaag
     7501 gctgttttct atttttctga ttattttgtt cattttctcc ctttttccgt attgttaatt
     7561 tcctatcgga gtccttacgc accgaaatcc aatgtaggac cgtccgtggt tctgatattc
     7621 cgaatcacgc atatccgagc ggataaaggt ggagatgtct ttccacgaac cgcctttcac
     7681 cacttttttc ttcaacggat aggggtcctc ggcagcggca ttataccggt attcggggtt
     7741 catgtcactc atgagttgat ttccggattc ggtgtaggcc gttgaggtcc attcggctac
     7801 attgcctgcc atatcgtaca aaccgaagct gttggggcgg tagcttttcg tcttggcggg
     7861 aatcaggttg cggtcggcag catatgcacc ttctcccggt ttgaagtttg cctggtaaca
     7921 atcttcctcg gcttgcgccg cggttggccc ccaagggtat ctgttttctg tttttccact
     7981 tcgggcggcc atttcccatt ctgcttcggt gggaaggcgg tatttttcaa cctttacatc
     8041 ccggcgctgc aatccccggc gaagaaacag ggtgcgccat tcgcagaacg cggttgcggc
     8101 ctcccacgaa acaccaacta caggatgatg tgcgtagccg gggtgagaaa aatagttgcg
     8161 catgtagtat tcattgttgg cgttgctaaa gtcgtttacc caacaggtgg tgtcgggata
     8221 gatgtttact atccgggtgt gtacaaaatc gtataaactg tttaacggac gcgtaatggt
     8281 ttcgttcacg atacgtcctt cttcgtcgat atatgctgta tcttttgata tcatgatcac
     8341 ctcatccgga tttgcggcga tatcggtatt taaaactctc tcctgcggat caagcctgtt
     8401 ttgtcgtttt gccgcttcgg cagcatcaaa ccattcgtag cggaaattca tctgacgggc
     8461 atccagcatt ttctttttgg taatcggatg tgtaatgtac acgctgttga tggccgcttc
     8521 ctcttcttcg gtatttcttg tccagggaat gggaatactc cagttgagac gaggttgtac
     8581 gggttcgccg tattcgtctt cggtaatcct gaaaaagtcg tctccggcat aagcagggtc
     8641 ggcaagacgt tcgcggatga tggagtcacg tacccagaag acaaactgtt tatactgcga
     8701 attggtaatc tcagcttcgt ccatccagaa gttatctacg gatacgccgc gggtaggaac
     8761 agtcatcccc cacaacgaat cgatctctcc gggacccatt cgataagagc cccgtgtgac
     8821 cagtaccata ttgtagggtg taggttcatt ccagactttt cctacgggaa ccccagtcag
     8881 ttcacctccc gtgcttattc caataccccg ccggccgcag gaggccagca caatgagaat
     8941 caggatcgga acaattaatt tttttattgt catacagttt aaaaagacat tagagccaag
     9001 agttaggata aaactttaaa cgttagatct taaagaagcc ggatactttt gtaaggcatc
     9061 ggttctctcc tgaacaaatc tatcgggaac cggtacctga agctcacttc gtgactacca
     9121 ccgcttcctt ttcctatgcc agatgtatac atgctgtaag cataacccat ttcaatattc
     9181 tttataatag ccccggcgaa taaggaacat ccttctttac cgtttcgaga cacacctgcc
     9241 gagtacttct tgccatatac catacgtacg gctgtatgca accgggtgtc ggttaagctc
     9301 gtcatcagaa gagccattgg ctgtatttca actaacggat gaaaaacgtt tatattgcat
     9361 ccggccatga aattatacga aaggggaatt cttgaatgga ttgaatcggc cttcgcgttt
     9421 tccggtgggc cgggacttcg cagtgggtaa aacgacggtt ggttaaggtg catagctgcc
     9481 agaccgacgt gaaaattccg ggttgtccac gaaattccgg cattcaggtc gagggatttt
     9541 ctatccgtag gctgggccct gatgattttc cggttgtttt tcgcagagtc ggtggtcagc
     9601 tgtagagaag ctgcatcgaa attgagattc agcattcccg cctgaacgcc gatacggagt
     9661 attccctttc cgaatttttg gttaaacgtg tattgggcgg caaagagggt gttttgttta
     9721 tttccggtga cgtttgaaag cgtttgaata ccgatgccgt gttgtaggcc caaaaactca
     9781 accggcatat ccatcgaaag aaaaagatgt ttgggcgcat ctttaatgtt tgtccaccgg
     9841 taattataga ttcccgaaat ctggatttta ccggtttcgc cggcaaaaga cgggttgtaa
     9901 taagtctttg ccgtccagaa ttgattgaac ggaacatccc attgcgcttg catttgagca
     9961 atgcagaaca ggaaaaatgc agacaaaaaa attaatcgcc ggttttccat tgaaagtata
    10021 acggaaaacc ggcgaaaaaa ttgtgcggtt catcacgggt ttatttcact ttcgtaggga
    10081 ttaatcacct tttacacgcc cgaaccgtag cattttcagc atccattttg gaagcggttt
    10141 ttcgggatta cggtttttca ggttgatgct caatcctagt ttcttcatca ccggaacttt
    10201 gtgtgtctcc acaaactcga tcagtgtccc gtccggatcc tcgatgtaag cgaattgccc
    10261 ggcggcatcg cccatatcaa acgaagcacc ttctttgaac ttttgggtgg tatctacggt
    10321 aaacggatgc cccagttcgg cacagtgttt ccccaatgca tccatgttcc ggatgtcgaa
    10381 gcagatctgg ataaatcccg gatcgcccca gaaacgtcct tcatagagtt tgcgcggcga
    10441 acgttccagg ggttgtacca gctcgatgta tgacggcccg aacagttcac tgaaactccc
    10501 ttttcgaggt tgggagtggg tgagcagccg gcggtggaac cgctgactgc ccgaaggcaa
    10561 tccttcaaaa tccgcgaaaa caccggtttc gtcggcgatg accctgtcgt agcccaggat
    10621 gtctttgtag acaaccattg ccttctcgat atcggttaca ccgattagag cacctactgc
    10681 acctccggta gaccggcctt cgtttttgaa aatatattcg tcttgaacaa tctggaacaa
    10741 attcccgtaa gggtccttca cgtagaaagt ttgtttaccg tctatgtcct gactcagttt
    10801 ggtgacgata ttgacttttg gatcccggga aaactccccg aaggttttcg cgacatccct
    10861 actttttatt ttgcacgcca gtacacccag atcgcccata ttcaattcaa aaccgagccg
    10921 ttttggtttt cttccggcat actgccatac ttcaaaaccg ccaccaccct gcagattcac
    10981 agcgattacc gcgtgacggc gctggggtac ccctccggta tatggaagca tcagttccgc
    11041 taccttgttg tcttccagga tgcggatatc catatgaaac acgtcgatgt aatatttcca
    11101 cgcttcgtgg aggttttcca caccgatacc gacttgctga atgcctgaaa taatatactt
    11161 attctccata gttgttttac aaaaagtgaa agggttacgt tgtcctgatt ttccgcacta
    11221 tttttcttgc caatcccgga aaaaaccgtt taatataaac catcagcaac tcttttccgc
    11281 cgaccagcac ttccggtttc tgcttccaga tggccttcac aatttttcgg gcggcttttt
    11341 ctgcaggtat accctccgct tgtccggcat ccatttgggc atgtctg
//
LOCUS       DAOSDR010000042        10953 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrimonas sp. isolate AMR_MDS_1826
            NODE_2299_length_10953_cov_4.003028, whole genome shotgun sequence.
ACCESSION   DAOSDR010000042 DAOSDR010000000
VERSION     DAOSDR010000042.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863355
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrimonas sp. (wastewater metagenome)
  ORGANISM  Petrimonas sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Dysgonomonadaceae; Petrimonas.
REFERENCE   1  (bases 1 to 10953)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10953)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:10:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,720
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 35
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10953
                     /organism="Petrimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2299_length_10953_cov_4.003028"
                     /isolate="AMR_MDS_1826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2023866"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..561)
                     /locus_tag="PLI30_02830"
     CDS             complement(<1..561)
                     /locus_tag="PLI30_02830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006841661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HOI78521.1"
                     /translation="MKLIHKAILINEGRSFTGSVLVEGDRISKVFENEVPEIILQQCG
                     EIIDARGLYLIPGVLDDQVHFRDPGLTHKGDIYSESRAAVAGGVTSFMEMPNTNPQTV
                     TIDALHEKFDLAAQKSIANYSFYLGATNDNIKELKKVDKKNVCGVKVFMGASTGNMLV
                     DNAKTLQRIFAEVDSLIATHCENEEII"
     gene            complement(558..1301)
                     /locus_tag="PLI30_02835"
     CDS             complement(558..1301)
                     /locus_tag="PLI30_02835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019537914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenol monophosphomannose synthase"
                     /protein_id="HOI78522.1"
                     /translation="MSDSLVIIPTYNEKENIENIIRTVFGLSKPFDILVIDDGSPDGT
                     ATIVRHLAETEFSSRLFMEEREGKLGLGTAYIHGFKWALARSYAYIFEMDADFSHNPD
                     DLPRLYSACHDEGFDMSVGSRYCSGVNVVNWPIGRVLMSYFASKYVQVITGVAIKDTT
                     AGFVCYKRKVLETINLDDIKFKGYAFQIEMKYTAYALGFKIKEVSVIFVNRQLGTSKM
                     NSSIFGEAFFGVMNLRWRKISGNIKPKQL"
     gene            1419..1491
                     /locus_tag="PLI30_02840"
     tRNA            1419..1491
                     /locus_tag="PLI30_02840"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1452..1454,aa:Met,seq:cat)
     gene            1709..2173
                     /locus_tag="PLI30_02845"
     CDS             1709..2173
                     /locus_tag="PLI30_02845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006798168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring lytic transglycosylase RlpA family
                     protein"
                     /protein_id="HOI78523.1"
                     /translation="MKKSFFFIIILFINTLNSPVFAQQKGGASYYADKFHGRKTSSGI
                     PYHRDSLTCAHKTYPFGTILEVINPKNGKKVLVEVTDRGPYSRNKIIDLSYAAAKQLD
                     IIHQGVTTVELSEWIWDLTRFKPFVFEFNNIFVKASHPAGKNLKIDKEKVLK"
     gene            complement(2192..4474)
                     /locus_tag="PLI30_02850"
     CDS             complement(2192..4474)
                     /locus_tag="PLI30_02850"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005796688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH92 family glycosyl hydrolase"
                     /protein_id="HOI78524.1"
                     /translation="MKSNSVFLLLLLSVYLVHAQDKLEPVDYVSILVGTQSKFELSNG
                     NTYPAIAMPWGMNFWTPQTGKMGDGWIYRYDADKIRGFKQTHQPSPWMNDYGQFSIMP
                     MTDKIEFNEEKRASWFSHKAEIAKPYYYSVYLADYDVTTEITPTERAAVFRFTFPETT
                     RSYVLVDAFDKGSAVEILAEKKAIIGYTTRNSRGVPENFKNYFVIVFDKPFEYKSVVK
                     SGEILQGIHKVEDNHAGAFIGFSTKKGEKVVAKVASSFISYEQAWQNLKEVESKSFEN
                     VKQEGRKSWNEVLGKVEIEDDNIDNKRTFYSTLYRSTLFPRKFYEIDAQGNAIHYSPY
                     NGQVLPGYMYTDTGFWDTFRSLFPLLNFMYPSVNKEIQEGLINTYKESGFLPEWASPG
                     HRGIMIGNNSASVVADAYLKGLRGYDIETLYEAMIHGTENVHPKVSSTGRLGHEYYNT
                     LGYVPYDVKIKENAARTLEYAYADWTIYKLAKALNRPQAEIDLYAKRCQNYRNLYSPE
                     YKLMRGKNKDGTFQSPFSPTKWGDAFTEGNSWHYTWSVFHDPQGLIDLMGGNKEFVNM
                     LDSVFKMPPVFDDSYYGFPIHEIREMQIMNMGQYAHGNQPIQHMVYLYNYAGEPWKTQ
                     YWAREVMNKLYTPAPDGYCGDEDNGQTSAWYVFSAMGFYPVCPGSDEYILGSPLFEKI
                     TIHLENGRKLLINSPGNSKSTRYISDFRLNGKTYTKNYVKHQDLMEGARIDVKMSDKP
                     NRTRGIRKSDFPYSFSNENK"
     gene            complement(4486..5931)
                     /locus_tag="PLI30_02855"
     CDS             complement(4486..5931)
                     /locus_tag="PLI30_02855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009136431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 125 protein"
                     /protein_id="HOI78525.1"
                     /translation="MTSRRNFIKKSALGLTALAVNPSWAKGKITTSGQAGKYVSRRPP
                     VSERKFVSRAVETTITRVKSKIKDEKLAWMFENCFPNTLDTTVNFSMRNGKPDTFVIT
                     GDIDAMWLRDSSAQVWPYLPLMNEDNQLKQMIAGVVNRQTQCILVDPYANAFYPNPNP
                     KGEWRTDNTDMKPEVHERKWEIDSLCYPIRLAYHYWKTTNDTSVFDADWRKAMALVLK
                     TFREQQRKENEGPYTFLRVTDRQLDTVNNKGLGNPVNPVGLIVSSFRPSDDATTFGFL
                     VPSNMFAVTSLRQLVEISESVTKDASFAKECKSLADEVDVAVKKYAVTTHPNHGQIYA
                     FEVDGFGNYYLMDDANVPSLLAIPYLGGECDDDAIYRNTRNFVLSKDNPYFFKGTAGE
                     GIGGPHIGYDMIWPMSIILRAITSQSDDEIRHCIQMLRDTDAGTGFMHESFHKNDPAN
                     FTRTWFAWVNTLFGELILKLEKENKMNLLNT"
     gene            complement(5938..7059)
                     /locus_tag="PLI30_02860"
     CDS             complement(5938..7059)
                     /locus_tag="PLI30_02860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008762719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 76 protein"
                     /protein_id="HOI78526.1"
                     /translation="MEGIGNEKQIADFILTNVIRLYDVPEHGLLSETYPINPDHKVNY
                     LAEGSTQKQKQEVSFLWPYSGVISGTVALYAFTKEKKYLDLMEKRLLPGLEKYFDTSR
                     HPGGYQSYPIFAGHSDRFYDDNVWLALDFCTFYANTQNKKYLDKALEIYDFVYSGWDG
                     NLGGGVYWCEQKKTSKNTCSNAPSAVLSARLYQLTGDEKFLSRAVETYLWTKNNLLDR
                     SDFVYWDNVALDGKIDKRKYTYNSGQMIEAGVLLYQITGDRTYLTDAQKTASGTYQYF
                     TTVESTAEGRTVFYPDTPWFNAILFRGLKALYIVDGNKKYIQSMLENAHFAWSNTADE
                     HNLLGKDWRKKSTQPYKWLLDNACMIELFAGLGDITKIK"
     gene            complement(7182..7646)
                     /locus_tag="PLI30_02865"
     CDS             complement(7182..7646)
                     /locus_tag="PLI30_02865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007766776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC ligand binding domain-containing
                     protein"
                     /protein_id="HOI78527.1"
                     /translation="MAYHDLDELDGKILKLIVNNARIPFLEVARECGVSGAAIHQRVQ
                     KLVNLGVIKGSEFILDAEKIGYETCAYVGLFLVSPSSFDSVVKELEKIPEVVECYYTT
                     GQYDLLIKVFAKNNKDLLRIIHSQLQPLGLARTETLMCFKDAFRKKLPIDFS"
     gene            complement(7736..8956)
                     /locus_tag="PLI30_02870"
     CDS             complement(7736..8956)
                     /locus_tag="PLI30_02870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009317985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HOI78528.1"
                     /translation="MPKVTDRMEMVNNAVMIRKESEKIFTEIVGHYRYLHQHPELSYK
                     EENTARYITEFLDIEKIPYRRNIGGYGILAWVVGEKEGGGKTAAFVADMDALPVHEKN
                     DVPYKSVTDGIMHACGHDSHTASLMGTAKIVNSLRSEFSGTVLFVFQPGEEQSPGGAA
                     LMLKDGVFRDFNPDFVIKQHAYVDLPAGMVAFQTGTVMASADEIHIKVKGEGGHGALP
                     HELNDTVLAASNILIALQQVVSRRRNPFQPMTLSFGKFIADGATNVIPSEVVLAGSLR
                     CMDESERQKMLGVIPQIIDSVSAAYGCSCEIVMPEGYPCTVSHAAVTEKVRSQAIDFL
                     GEAGVSEYPVRMTAEDFGFFSQQYPCCFYRFGVADRSGACGKLHSSNFLIDEKALLTA
                     SSLFAFIALDSLER"
     gene            complement(8970..10214)
                     /locus_tag="PLI30_02875"
     CDS             complement(8970..10214)
                     /locus_tag="PLI30_02875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016277481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1015 domain-containing protein"
                     /protein_id="HOI78529.1"
                     /translation="MAIIKPFKGVRPPKQFVKEVASRPYDVLNSDEARREAEGNEKSL
                     YRIIKPEIEFPVGKDEHDEDVYAKAAENFRLFQDKGWLVQDEKEMYYVYAQTMNGKTQ
                     YGLVVGAYVEDYINGKIKKHELTRRDKEEDRMKHVRVNDANIEPVFFAYHYNEEINAI
                     IARIIRQEAEYDFISVDGVGHHFWLIDNDTDIRRITEIFATFPAMYIADGHHRSAAAA
                     LVGAEKAKNNPYHRGDEEYNYFMAVCFPDNQLTIIDYNRVVKDLNGLSPDAFLKKLEK
                     NFLVEPTGSEIQKPGNLHNFSVYLDGRSFSVTAKPGTYDDNDPIGALDVTVTSLLILD
                     EILGIKDLRSDKRIDFVGGIRGLGELKKRVDTGEMALAIALYPVSMKQLMDIADSGNI
                     MPPKTTWFEPKLRSGLVIHKLS"
     gene            10308..10604
                     /locus_tag="PLI30_02880"
     CDS             10308..10604
                     /locus_tag="PLI30_02880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI78530.1"
                     /translation="MSNQTLSIMGGEGHMLDMIKKLELNRTQRKEVGGGRFRSHKRPR
                     ITEEYLRNIESVEIEKPEEISSQKKRQYQIIAIVSLIIVVAALLFFFIQLFSHR"
     gene            10667..>10953
                     /locus_tag="PLI30_02885"
     CDS             10667..>10953
                     /locus_tag="PLI30_02885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019537928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4375 domain-containing protein"
                     /protein_id="HOI78531.1"
                     /translation="MIQIPEQKIIKAAEKGMDEFLKVFTDAYLEVLDGGITAENMHKL
                     NGYQHTLLAFRFFTDEVREGGFVQLIQNGYGGYLFDNPAAKALKSMGAKGLS"
BASE COUNT         3046 a         2547 c         2174 g         3186 t
ORIGIN      
        1 tataatctct tcgttctcgc aatgtgtagc aatcaacgaa tctacctctg caaagatccg
       61 ctgcagcgtt tttgcgttgt ctaccagcat gtttccggta gaagcgccca taaatacttt
      121 caccccgcaa acgttctttt tgtcgacttt tttcagttcc ttgatgttgt cgttggtggc
      181 acccaggtaa aacgaataat tggcaatgga tttttgtgcg gccagatcaa acttttcgtg
      241 caaagcatct atcgtcacgg tttgcgggtt ggtattgggc atttccataa acgaggttac
      301 gcctccagcg acagcggccc tgctctccga ataaatatca cccttgtgcg taagtccggg
      361 atcacgaaaa tgcacctgat catccaatac cccgggaata aggtacaagc cccttgcatc
      421 gataatttca ccgcactgct gaaggataat ttcggggact tcgttctcaa atactttcga
      481 aatcctgtct ccttctacaa gtacagaacc cgtaaaggaa cggccttcgt taataaggat
      541 tgccttatgg ataagtttca taattgtttg ggtttaatgt tgcccgatat ttttctccag
      601 cgcaaattca taacaccaaa aaaagcttcc ccaaaaatgc tgctgttcat tttggaggtc
      661 cccaactgac gatttacaaa tatcaccgat acttctttta ttttgaatcc caatgcgtaa
      721 gccgtatact tcatctcaat ctgaaacgca tagcctttga attttatatc gtccaggttg
      781 atggtctcca atacctttcg tttgtagcaa acaaatccgg cagtagtatc cttaatagct
      841 acccctgtta tcacctgaac atatttggag gcgaaataag acatcaagac acgtcctata
      901 ggccagttga caacatttac accgctgcaa taccgggatc ccaccgacat atcaaaccct
      961 tcatcgtgac aggcagaata aagccttggc aagtcatcgg gattgtggga aaaatcggca
     1021 tccatctcaa aaatataagc gtaagaacgg gctagagccc atttaaaacc atgaatataa
     1081 gcggtcccca aacccaactt gccttcccgc tcttccataa aaagccggct tgaaaattcc
     1141 gtttctgcca aatggcgtac aatggttgcg gttccatcgg gcgacccatc gtctataacc
     1201 agtatgtcaa atggctttga caaaccaaaa acggtacgga taatgttctc gatgttttct
     1261 ttttcgttgt acgtgggtat aattacaaga ctgtcggaca tggttttgaa atttatacca
     1321 aaagtacata ttttgtcgca gaaaaaaaaa gaaatgcaaa aaacttgttt tataaataaa
     1381 aacattgtat ctttgcacct caattaatga aaacatatcg cggagtggag cagttggtag
     1441 ctcgttgggc tcataaccca aaggtcatag gttcaagtcc tatctccgca actactgaag
     1501 gaaagtttta atactttcct tttttctttt ctacgggtta acgcgtcaat cttcccaata
     1561 tctcctgcat ctccggatct ggtttgtgag cctgcagata accacgataa aaaatgatct
     1621 ggtatcttta tgggcaaaaa aaataaaaaa aatgggagac tctcattttt tttatacatt
     1681 tgcagtgtga aaatcaaaac aagagacaat gaaaaagtcg ttctttttta ttatcattct
     1741 gtttataaac acattaaata gccctgtttt tgcacagcaa aaaggaggag cgtcctatta
     1801 tgccgacaag tttcacggcc ggaaaacatc aagtggcata ccctaccata gagacagcct
     1861 cacttgcgct cacaaaacct atccgtttgg gaccatactg gaagtgataa atccgaaaaa
     1921 cggaaagaag gtactggtgg aagtaaccga tcgcggccca tactccagaa ataaaatcat
     1981 cgacttgtcg tatgccgctg ccaagcaact ggacattatc catcaaggtg tcactacggt
     2041 ggagctcagc gaatggattt gggacctaac aagattcaag ccgtttgttt tcgaattcaa
     2101 caatatcttt gtgaaagctt ctcacccggc agggaagaat ttgaaaatag acaaagaaaa
     2161 agtgctgaag taaatttcag cacttttttt gttatttatt ctcgttggaa aacgaatacg
     2221 gaaaatcgga ctttcggatt ccccttgtcc tgttcggttt atccgacatc ttcacatcaa
     2281 ttctggctcc ttccatcaaa tcctgatgct tcacgtagtt tttggtatac gtttttccgt
     2341 tcaatctaaa gtccgaaatg taacgagttg acttactgtt ccctggagaa tttatcagta
     2401 gttttctccc gttttccagg tgaatggtta ttttctcaaa caacggtgac cccaggatat
     2461 attcatcgct tcccggacaa accgggtaaa atcccatagc agaaaaaaca taccatgccg
     2521 acgtttgccc gttatcttca tcaccgcaat aaccgtcggg agcgggcgta taaagtttat
     2581 tcatcacttc acgcgcccag tattgggttt tccagggttc tcctgcataa ttgtagaggt
     2641 ataccatgtg ctgaatgggt tgattcccgt gagcatactg ccccatattc ataatctgca
     2701 tctcccggat ttcgtgaata ggaaatccgt aatagctatc gtcaaaaacc ggtggcatct
     2761 tgaaaacaga atccagcata ttcacaaatt ccttgtttcc acccatcaga tcgatcaatc
     2821 cttgcggatc gtgaaatacc gaccaggtgt agtgccaact gttgccttcg gtaaatgcat
     2881 ccccccattt cgtggggctg aaaggcgact gaaacgttcc gtccttgttc ttgcccctca
     2941 tcagtttgta ctcaggtgaa tacaaattac ggtaattctg acaacgtttc gcgtataggt
     3001 cgatctccgc ctggggacgg ttcaaggctt tggccaattt ataaattgtc cagtcggcat
     3061 acgcgtattc cagcgtgcgc gccgcatttt ctttgatctt cacatcataa ggaacgtagc
     3121 caagcgtatt gtaatattca tgccccaacc gcccggtaga cgagaccttc ggatgtacat
     3181 tctccgttcc gtgtatcatt gcctcataaa gcgtttcgat atcgtagccg cgcaaccctt
     3241 tcaggtaagc atcggccacc accgaagccg aattgttccc aatcatgatt ccacggtgtc
     3301 caggacttgc ccattcaggc agaaagccgc tctctttgta cgtattaatc aacccctcct
     3361 gaatctcctt gttgacagac gggtacataa aattcagcaa aggaaacagg gaacggaatg
     3421 tatcccaaaa tccggtatcg gtgtacatat atccggggag tacctgcccg ttgtaagggc
     3481 tataatgtat tgcatttccc tgggcatcaa tttcgtaaaa cttacgggga aagagcgttg
     3541 aacgataaag ggtggagtaa aaagtgcgtt tgttgtcaat attgtcatct tcaatctcta
     3601 ctttgcccaa tacttcattc cacgattttc tgccttcctg cttaacattt tcgaaactct
     3661 tgctttcaac ttcttttaaa ttctgccagg cttgttcgta gctgataaaa gaggatgcca
     3721 cttttgcaac gactttctcc ccttttttcg tggaaaatcc gataaaagca cctgcatgat
     3781 tgtcttccac cttatggatc ccttgcagta tctctccgct tttcactaca gatttatatt
     3841 caaagggttt atcaaaaaca atcacaaagt aatttttgaa gttttccggc actcccctgc
     3901 tgttacgtgt agtataaccg atgatggctt ttttttccgc aagaatctcc acggccgaac
     3961 ccttgtcgaa agcatccacc agcacatacg aacgggttgt ttccgggaaa gtaaagcgaa
     4021 agacagccgc gcgttccgtt ggcgtaattt cggttgtcac atcgtaatca gccaggtaaa
     4081 cactgtaata ataaggtttg gctatttcag ccttgtgtga aaaccaactg gctcgcttct
     4141 cctcattaaa ctcaatttta tccgtcatcg gcataatcga aaactggccg taatcattca
     4201 tccatgggct tggctgatgc gtttgcttga atccgcggat tttatccgca tcgtagcggt
     4261 aaatccatcc atcacccatc tttccggtct gcggagtcca gaaattcatt ccccacggca
     4321 tggcaatggc cggataagta tttccgttgg aaagttcaaa cttggattga gttcctacaa
     4381 gaatactcac ataatcaacc ggttctaatt tatcctgtgc gtgaacaaga taaacgctca
     4441 gcaacaaaag aagaaacact gaatttgatt tcattacgct tatttttaag tgtttaatag
     4501 gttcatttta ttttccttct ctaatttaag aatcaattct ccaaaaagag tgttaaccca
     4561 tgcgaaccat gttcgtgtaa aatttgcagg atcattttta tgaaacgatt catgcatgaa
     4621 tccggtaccg gcgtcagtat cacgaagcat ttgtatacaa tgtcgaatct catcgtcgct
     4681 ctggcttgta attgcacgca aaataatact catgggccaa atcatatcat aaccgatgtg
     4741 tggtccacca attccctccc ctgcagttcc tttaaagaaa tacggattat ctttgctcaa
     4801 cacgaaatta cgcgtatttc ggtagatagc gtcatcgtca cattcgccac ccaagtacgg
     4861 aattgccaat aaacttggga cattagcatc gtccatcagg taataatttc caaatccgtc
     4921 cacttcaaat gcgtagattt gaccgtggtt gggatgtgtg gtgaccgcat atttcttaac
     4981 agctacatca acctcgtcgg ccagggattt gcactctttc gcaaaagaag catccttggt
     5041 aaccgattcc gaaatttcga ccaattgcct cagcgaggtt acggcaaaca tattggaagg
     5101 aaccagaaat ccaaaagtgg tagcatcatc cgaaggacgg aacgaagaaa cgattaatcc
     5161 gacaggattc acaggattcc ccaatccctt attgtttacc gtatccaact ggcggtcggt
     5221 aacacgtagg aaagtatatg gcccttcatt ttcttttcgt tgttgttccc tgaaggtttt
     5281 caataccaat gccatcgctt ttcgccaatc ggcatcaaag accgaggtat cgttggtcgt
     5341 tttccaataa tggtaagcca gacgaatagg ataacaaagt gaatcaattt cccacttgcg
     5401 ctcgtgcacc tcgggtttca tatcggtatt gtccgttctc cattcccctt tgggattagg
     5461 attaggataa aatgcgttcg cataagggtc gacaagtata cactgcgttt gcctgtttac
     5521 aacgcctgca atcatctgtt tgagttgatt gtcttcgttc attaaaggaa gataaggcca
     5581 aacttgcgcc gaggagtcac gaagccacat ggcatcgatg tcacccgtaa ttacaaaagt
     5641 atcgggtttc ccgtttctca ttgaaaaatt cacggttgta tccaacgtgt tcggaaaaca
     5701 gttctcgaac atccaagcca atttttcatc tttaatcttg gacttaaccc ttgtgatggt
     5761 cgtttccaca gcccttgaaa caaattttct ttcagaaaca ggaggccgtc gtgacacata
     5821 tttacccgcc tggccggaag ttgtgatttt tcctttcgcc caggaaggat ttacagccaa
     5881 ggccgtaagc cccagggcac tttttttgat aaaatttctc cttgaagtca tttttattca
     5941 cttaatttta gtaatgtcgc cgagtccggc gaaaagttca atcatacaag cattatcgag
     6001 caaccattta tagggttgtg tcgacttttt cctccagtct tttccaagca ggttatgctc
     6061 atctgctgta ttgctccagg caaagtgagc gttttcaagc atggattgaa tatatttctt
     6121 gtttccgtcc acgatataaa gcgcttttaa tcccctgaaa agtattgcat taaaccacgg
     6181 agtatcggga tagaacactg ttcttccctc cgcagttgat tcaacagttg taaaatattg
     6241 ataagttccc gatgctgttt tttgagcatc agtcaggtat gttctgtcac cggtaatctg
     6301 gtaaagcaaa actcccgctt cgatcatttg cccgctgttg taagtatatt tccttttatc
     6361 aatttttcca tcaagcgcaa cattatccca ataaacaaaa tcagaccggt ccagtaaatt
     6421 gttttttgtc cagagataag tctctactgc acgtgaaagg aatttttcat cgccggttaa
     6481 ctgatacaac ctggcactga gtacggctga cggagcattg gagcaggtgt ttttactggt
     6541 ttttttttgc tcacaccaat aaactccccc acccaggtta ccgtcccatc cgctgtaaac
     6601 aaagtcatat atttcaagtg ctttatcgag gtatttcttg ttttgagtat ttgcgtagaa
     6661 cgtacaaaaa tcaagtgcca gccatacatt atcatcatag aacctatcac tgtgtcccgc
     6721 aaaaatagga taagactgat aacctccggg gtgacgcgat gtatcgaagt atttctccaa
     6781 ccccggcaac aagcgcttct ccatcagatc aaggtatttt ttttccttcg taaaagcata
     6841 cagggcaacc gttccagaaa tcacacccga gtaaggccat aaaaaagaca cttcctgttt
     6901 ttgtttttga gtgctcccct cagccaggta atttactttg tgatcgggat ttatcggata
     6961 ggtttccgac aacaatccgt gttcaggaac atcataaagt cttataacat tagtgagaat
     7021 aaagtcagca atctgctttt cattgcctat tccttccact tttttacctg gaactccggc
     7081 attaccagca cagaatagca aaccggtaaa aacaaaaata aatatcaata tttttttttt
     7141 gcccaccatc tgactttgaa ataaaacagg taataatttt tttacgaaaa atctatcggc
     7201 aatttcttgc gaaaagcatc tttgaagcac atcaacgttt cggttctggc cagaccaaga
     7261 ggttgaagct gactgtggat aatacgaagt agatctttgt tgtttttagc gaacacttta
     7321 attaacaggt catactgccc ggtagtgtaa taacactcca ccacttcggg gattttctcc
     7381 aattctttca ctacgctatc gaaagacgaa ggcgatacaa gaaacaaccc cacataagca
     7441 cacgtttcgt aacctatctt ttcagcatca aggataaatt cagatccctt gataacgcct
     7501 aaattgacta atttctgtac gcgttggtga atggctgcac ccgacacacc acattcgcgg
     7561 gctacttcaa gaaagggaat tcgtgcgttg tttacaataa gtttcaggat tttcccatcc
     7621 agttcgtcta aatcatgata agccatagtt atacttttaa tttgttagaa aaatgattat
     7681 ttttttacaa aaatataaaa aaacaggttc ctaacagcaa aaaacgaata aattattacc
     7741 tttcaagtga atcgagagca ataaaagcaa ataacgatga agcggttaac aatgcctttt
     7801 cgtcgatgag aaaattggat gagtgaagct tgccgcaagc cccgcttctg tcggccacac
     7861 caaaccgata aaaacagcac ggatattgct gggaaaaaaa gccaaaatcc tctgctgtca
     7921 tgcgtacggg gtactccgaa accccggctt cgcctaaaaa atcaatagcc tgcgaacgta
     7981 ctttttcagt tacagcagca tgactgacgg tgcacggata accttcaggc ataacaatct
     8041 cacagctaca cccataggca gcagataccg aatcaataat ttgcgggatg acaccaagca
     8101 tcttctgtcg ttcgctttcg tccatacacc tgagtgagcc ggcaagcact acttccgaag
     8161 gaataacgtt ggttgcaccg tcggcaatga atttcccgaa agaaagtgtc atgggttgaa
     8221 acggatttct gcgacggctt accacttgtt gaagagcaat aaggatgttc gaagcagcca
     8281 gcaccgtatc gttcagttca tgaggtaatg cgccgtggcc accctctcct tttaccttaa
     8341 tgtgaatttc atcggcagat gccattaccg ttccggtttg gaatgccacc attccggctg
     8401 gtaagtccac ataagcatgc tgttttatta cgaaatcggg gttaaaatcc cggaaaacgc
     8461 cgtcttttaa catcaaggcg gctccaccgg gcgattgttc ctctccaggt tgaaacacaa
     8521 acaataccgt gccggaaaat tcacttcgca gcgaattcac tattttggct gtccccatca
     8581 acgatgccgt gtgtgaatcg tggccgcagg catgcatgat accatcggtc accgatttat
     8641 acggcacatc atttttttcg tgtacaggca aagcatccat atcggcaaca aaagcggcgg
     8701 tttttccacc cccttctttt tcacctacga cccacgccag aattccatat cctccgatat
     8761 ttctgcggta cggaattttt tcgatatcca aaaactccgt aatgtacctt gccgtatttt
     8821 cttctttata ggaaagctcg ggatgttgat gtaaatagcg gtaatgtccg acaatttcgg
     8881 taaaaatttt ttccgattct tttcttatca ttaccgcatt atttaccatt tccatccggt
     8941 ctgttacttt gggcattatt ccctgtgatt tacgaaagtt tatggattac caggcctgaa
     9001 cgaagtttcg gttcaaacca cgttgttttg ggaggcatga tgtttccaga atcagcaata
     9061 tccatcaact gcttcatgga tacaggataa agtgctatcg ccaatgccat ttctccggtg
     9121 tccacccgtt ttttcaattc tcccaagcca cggattcccc ccacgaaatc aatacgctta
     9181 tcggaacgca ggtcttttat tcccaaaatc tcgtccagga taaggaggga tgtaaccgtt
     9241 acatccagtg cacctatagg gtcgttatcg tcataggttc cgggttttgc cgtcacactg
     9301 aatgaacgtc cgtcgagata taccgagaag ttgtgtaagt ttccgggttt ctgtatttcg
     9361 gaaccggtag gctcaaccag gaaattcttt tccagttttt tcaaaaatgc gtcaggggat
     9421 aatccgttca aatcctttac cacccggttg taatcgataa ttgtcaactg attgtccgga
     9481 aaacagaccg ccataaaata gttatactcc tcatcccccc tgtggtaagg attattcttt
     9541 gccttttcag cacctacaag ggcagccgct gcggagcggt ggtgtccatc agcaatatac
     9601 atggccggga aagtggcgaa aatttcggta atgcggcgga tatcggtatc attgtcaatc
     9661 agccagaaat ggtgtcctac accgtctacg gaaataaaat catattcagc ttcctgcctg
     9721 ataattcgtg ctattatggc attaatctct tcattgtaat ggtaagcaaa aaatacgggt
     9781 tcaatattgg catcgttcac acgcacatgc ttcatccggt cttcttcttt atcgcgccgg
     9841 gttaactcat gtttcttgat tttaccgttt atgtaatctt ccacgtaagc accgaccacc
     9901 agaccatatt gcgtcttgcc gttcattgtc tgtgcataca cgtaatacat ttctttttcg
     9961 tcctgcacca accagccttt gtcttgaaac aaccggaaat tctctgcagc cttcgcgtac
    10021 acatcctcgt cgtgttcatc ttttccaacc ggaaattcaa tttccggttt aatgattcga
    10081 tagagcgatt tttcatttcc ttcggcttcc cggcgggcct cgtcagaatt tagcacatca
    10141 tacggacgcg atgcaacctc ttttacgaat tgtttgggtg gacgaacacc cttgaatggt
    10201 ttaataattg ccattgttta agattaatat tatttgtgaa tactgctgca aaagtactgt
    10261 ttttttccca accttgtagt tattcagcga aattgtttaa ctttggtgtg agcaatcaaa
    10321 cacttagtat tatgggtggt gaaggacaca tgctggacat gataaagaag ctggagctaa
    10381 accgcacaca aagaaaggag gtcggaggcg gaagattcag gtcgcataag cggccgcgca
    10441 taacggaaga atacttacgg aacatagaaa gcgttgaaat agaaaaacct gaggaaattt
    10501 cttcacaaaa aaaacggcaa tatcaaatta ttgccattgt ctccctgatt attgttgtgg
    10561 cagccctact ttttttcttc atccagctat tttcacaccg atgagttttt tatacggcag
    10621 gtaacacctt caaaatttat taaaaacaga taaaacgtga agtaatatga ttcaaattcc
    10681 cgaacaaaag attatcaagg cagccgaaaa aggcatggat gaatttctga aggtgtttac
    10741 cgatgcttac ctggaagtgt tagacggggg cattacggca gagaacatgc ataagctaaa
    10801 tggttaccaa cacacgcttc tggctttccg gttttttacg gacgaagtgc gcgaaggggg
    10861 tttcgtgcag ttgatccaga acggatacgg cgggtacctt tttgacaacc ctgcggcgaa
    10921 ggccttgaag tcgatgggag ctaaagggtt atc
//
LOCUS       DAOSDR010000043        10951 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrimonas sp. isolate AMR_MDS_1826
            NODE_2301_length_10951_cov_4.463840, whole genome shotgun sequence.
ACCESSION   DAOSDR010000043 DAOSDR010000000
VERSION     DAOSDR010000043.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863355
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrimonas sp. (wastewater metagenome)
  ORGANISM  Petrimonas sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Dysgonomonadaceae; Petrimonas.
REFERENCE   1  (bases 1 to 10951)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10951)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:10:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,720
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 35
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10951
                     /organism="Petrimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2301_length_10951_cov_4.463840"
                     /isolate="AMR_MDS_1826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2023866"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1739
                     /gene="alaS"
                     /locus_tag="PLI30_02890"
     CDS             <1..1739
                     /gene="alaS"
                     /locus_tag="PLI30_02890"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018074873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HOI78532.1"
                     /translation="SITDGQLPSNAKAGYVIRRILRRAVRYGYTFLNMHEAFMYKLVP
                     TLIEVMGDAYPELETQKTLLGKVIKEEENAFLRTLETGIRLLDKEIAEHKRSGRNELE
                     GEIAFQLYDTFGFPLDLTELILREHDMSVDHKGFDAEMQKQKERARNAAAVETGDWIK
                     IRDGETEFVGYDETECETQVIRYRKVKQKNKEYYQIVLTKSPFYAEMGGQVGDSGWLI
                     DENGEGTDIFDTKRENNLPVHLANKLPHHIEGSFRAKINTARRTATECNHTGTHLMHE
                     ALREVLGTHVEQKGSYVSPEILRFDFSHFQKMTPEEIRAVEQNVTEKIRSNFTIEEHR
                     HVPIDEARAQGAMALFGEKYGDDVRTVRFGSSIELCGGTHISSTGRIGTFRIVSESAI
                     AAGVRRIEAVTAKGCEDYLYTMEDMLRNIKEALSGNAPEIMVGLRKLMSENEEMRQKI
                     EAFTKERTVQLKNLIIQKKEIVNGVTVFKLEGMVVTPEIVKDIAFQLRGEFPEYMAFM
                     AATGGNNKPSLTVMLSDDLVSGGLNAGTIVREAAKHIQGGGGGQPHFATAGGKNADGL
                     KMAMETMLQYLP"
     gene            1817..2833
                     /locus_tag="PLI30_02895"
     CDS             1817..2833
                     /locus_tag="PLI30_02895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006842443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HOI78533.1"
                     /translation="MDKRQITLKDIAKELKLSPSTVSRALRNHPAISKETKELVRDYA
                     VKHKYKPNTLALQLRTNRNNTIGVIVPEIVHYFFSTVIAGIQDEAEKENYNLIICQSN
                     ENYGREVKSVETLLDARVRGILASQSKSTEEFSHFQDIIDNGTHLVFFDRICTGINTD
                     KVVIDDYAGAYNAVDYLIGSGCTRIAFLGSNPHLPIANNRRMGYEDALRNHNIPVDKS
                     LMQVCDTIELARETVPVMLKITPVPDAFFCINDEVASYCLQICKSAGYKIPQDISVCG
                     FTNSYITEVTDPTLTSVDQHGFEMGKTAARLLINRIKGIEKKTGIVSKVIKTKLVVRN
                     STRN"
     gene            2977..4512
                     /locus_tag="PLI30_02900"
     CDS             2977..4512
                     /locus_tag="PLI30_02900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019538630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOI78534.1"
                     /translation="MKKRHLSFWEIWNMSFGFLGIQFGFALQNANVSRIFETLGARVE
                     DIPILWIAAPLTGLIVQPIIGHLSDKTWNRFGRRRPYFTIGAIFTTLALFVMPNSPAL
                     WIAAGMLWIMDASINISMEPFRAFVGDNLPSEQRTMGFAMQSFFIGLGAVVASALPYI
                     LTNWFGIPNTAGEGIIPQSVKLSFYIGGVVLLASVLYTVFTSKEYSPEELAAFEQEKE
                     KISGIKTEIKPPVTPVQFLKNGSVWTTAGIIATAIIVLLKLEAQLYIVSGLVLTFGII
                     LLVSGMLTKKGRTQNGMVGIMNDLLHMPRTMGQLAVVQFFSWLSFYAMWIYTTPAVTQ
                     HVFGTNDTASELYNQGADWVGVLFAVYNAVSALSAFLLPVLARQIGRKATHSIALALG
                     GISFISFFFIQNPQILLLPMIGIGFTWGSILSMPYAILTGSLPADKMGTYMGIFNFFI
                     VIPQILAASILGFVTKDIFGGQVIFTMVLAGCSLVLGALSVFFVQDRDDAYKFKKNAQ
                     EQK"
     gene            4564..5904
                     /locus_tag="PLI30_02905"
     CDS             4564..5904
                     /locus_tag="PLI30_02905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009137962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HOI78535.1"
                     /translation="MKKTIFLLWIVSLLFSCGNKKQEAATSVHPEWVYDATIYEVNTR
                     QFTPEGTFNTFAAHLPRLKELGVDLLWFMPVQPIGEKDRKGTLGSYYSIRNYTEVNPE
                     FGTMDDFITVVKEAHDMGMKVILDWVPNHTSRDHTWITEHSDWYKRDSLGSPIIMYDW
                     TDIAPLDYGKNEMRKAMIDAMAFWVREADIDGFRCDVAYEVPIDFWETAKDSLITLKP
                     DIFMLAEAEKPELNESIFDAYYAWDLHHKMNMVAQGKENVDSLRLSLKRMNERFSSHA
                     IPMYFTSNHDENSWNGTEFERMGEAAGTFAVLTYMLPGMPLIYSGQEAGLNRRLQFFE
                     KDSINWTDRGGFTGLYTSLNALKKTNKALLSPERGGKMTEIVNDSPASVFSFKRAHEG
                     NEIVCVFNLSKYSVKTVFNEEVPGAGFTAFPSAETVTPVKKMELAPWEYRIYVK"
     gene            complement(5917..6861)
                     /gene="corA"
                     /locus_tag="PLI30_02910"
     CDS             complement(5917..6861)
                     /gene="corA"
                     /locus_tag="PLI30_02910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006799731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015087 - cobalt ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006824 - cobalt ion transport [Evidence
                     IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HOI78536.1"
                     /translation="MAEIKLFYHKDGVVRLSRSLDFLKSNPIQSFLWIDLNDVDEEVE
                     NELEDFLKIYIQEEEEMIEIEMSSRYIETQDTLVVNSNFLLSNFESDPVSFILKNNIL
                     VTVRSEELISFHETVKKISANPKGYNTGADVLVALLETRVEFDADMIENMTHKITQLS
                     NSLSLQEADKELLGEIKNLQEKTMMLRENIIDKQRTVSSMLRSDFIPKELQPKLSMVI
                     RDINSLVEHIKFSFDRLDYLQDTFLGYVNIEQNKIIKIFTIVSVIFMPPTLIASIYGM
                     NFTAMPELNMKWGYPVSIGLMVLSSLAILLYFKKRKWL"
     gene            complement(6890..7696)
                     /gene="rsmA"
                     /locus_tag="PLI30_02915"
     CDS             complement(6890..7696)
                     /gene="rsmA"
                     /locus_tag="PLI30_02915"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019538463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HOI78537.1"
                     /translation="MISVKPKKNLGQHFLKDSAIASRIADTLDAFAPLPILEVGPGTG
                     MLTQFLLSKGRNLTVVEIDRESIAYLKEYFPSLNGNILQQNFLTLDVAEYYDGPFCVI
                     GNYPYNISTQIFFKVLENKDLIPCCAGMIQKEVAERMIASPGSKAYGILSVLLQAWYD
                     IEYLFTVSEQAFIPPPKVKSAVVRLTRNKRKQLNCNEKLFKSVVKTSFNQRRKMLRNS
                     IRSLLPEESPLPDDPMLTKRPEQLSIEQFEQLTNLLEPLAGKNFLEENKN"
     gene            7866..8888
                     /locus_tag="PLI30_02920"
     CDS             7866..8888
                     /locus_tag="PLI30_02920"
                     /inference="COORDINATES: protein motif:HMM:TIGR00374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HOI78538.1"
                     /translation="MNSKRIQDILKTVLSLALGVAIIWLLYRKTNLREVWEIASSAHF
                     GIIASSLVFGLLGNYFRALRWELFLNSLGYRPKRESIVFATFGNYAVNFLLPRAGDLW
                     RCGIVTKYDQIPFSKTFETFFVDKILDILAGVVLVIISLALYVDFFVSYFHQNPGFAE
                     NFTKLFTSVWLYLLLILISGGIFVMFFYFKENPLVKKVNKFLQVVQYDMVLIAKMKSK
                     GKIIIYTVLAWLSFYVYFYICFYAFDFTKNLGPIIGWIVYAMSNVGVAVPVQGGVGTW
                     HFMVISSLVIFGIGYEEASAFAGAIFTVQSVWIILLGVMGILALPYVKREKENLGLPV
                     IENQKQ"
     gene            8899..10362
                     /locus_tag="PLI30_02925"
     CDS             8899..10362
                     /locus_tag="PLI30_02925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019538465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-histidine dipeptidase"
                     /protein_id="HOI78539.1"
                     /translation="MSTSEILNLNPQGVWKYFHELNQIPRPTGQMEEVTKFVMNFGKS
                     LNLETRQDKAGNVLIKKPASKGYESGKTIIIQSHLDMVPQKNADVTHDFSRDPIQTYI
                     DGEWVKARSTTLGADNGIGCAMMMAVLEDNLLNHPPIEALFTVDEEVGMDGAFGLQKG
                     FLSGTVMLNLDTEEDGDLCVGCAGGTDVNVSFQFKPDEEIEKGDVAFKISLTGLKGGH
                     SGTQIHLGHANANKLMNRFLKDVVRNYEARLANIHGGSLRNAIPRESFAVVTIPEQLS
                     EDLVDLVNEYESLFREEFSGIESSISFKAEKTDVPESLLPIEVQDDLINAVEGCPNGV
                     ISMLAEFPGVVESSLNLALVQSTEGQIEVKLLVRSSSESRKEWVCSSVESVFMLAGAK
                     VEFDGAYPGWQPDANSELLTTMTKIYMEKYGQRPNVNVIHAGLECGIIQANAGHKLDI
                     VSFGPTITGAHSPDEAVHIAAVGKSYDYLLAILEQMK"
     gene            10404..10910
                     /locus_tag="PLI30_02930"
     CDS             10404..10910
                     /locus_tag="PLI30_02930"
                     /inference="COORDINATES: protein motif:HMM:NF024135.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin domain-containing protein"
                     /protein_id="HOI78540.1"
                     /translation="MAANNTLIVYASRRGDEEKCAREIFNLIDGKVDICDLSNRESFP
                     DASTYDTVIIGSSVYHGKVLELVSNFCRINLDILTRKRLGLFIICPDSGERAEKELQE
                     AYPPDLFNSAIARDYFGGEIDPYRLSILEKIITYRLSGPRKPSIPASREKIKKFAKKM
                     NPSHASQE"
BASE COUNT         3015 a         2501 c         2578 g         2857 t
ORIGIN      
        1 tctcgataac cgacggccaa ttgccatcga atgccaaagc cggatacgtt atccgccgta
       61 ttttgcgccg cgccgtccgt tacggataca cgtttctcaa catgcatgaa gcgtttatgt
      121 ataagcttgt gcctacgctc attgaagtta tgggagatgc ctatcccgaa ttggagacgc
      181 aaaagacact gctaggaaag gtgattaaag aagaagagaa tgcgttctta agaacgctcg
      241 aaaccggaat ccgtttgctt gacaaggaaa ttgctgagca caagagatcg ggcaggaatg
      301 aactggaggg agagatcgct tttcagctat acgacacttt tggcttcccg ctcgacctga
      361 ctgaacttat tcttcgtgaa cacgatatgt cggtcgacca caaaggcttt gatgccgaaa
      421 tgcaaaaaca gaaagaacgt gcacggaatg cagctgccgt ggaaacggga gattggatta
      481 aaatacggga cggcgagacc gaatttgtag gttacgacga gaccgaatgc gaaacgcagg
      541 tgatccgtta ccggaaagtg aaacagaaaa acaaggaata ttaccagatt gtgcttacca
      601 agtcgccgtt ttatgccgaa atggggggcc aggtaggcga cagcggctgg cttatcgacg
      661 aaaatggaga ggggaccgac atcttcgaca ccaagcgtga gaacaatctt cccgttcatt
      721 tggcgaacaa gctgccgcat catatcgaag gctcttttcg agcaaaaata aacaccgcca
      781 ggcgtacggc aaccgaatgc aatcacaccg ggacacacct gatgcacgag gcgctgcgtg
      841 aggtactggg aacgcatgta gagcaaaaag gatcgtatgt ctctcctgaa atcctgcggt
      901 tcgacttctc gcactttcag aaaatgaccc cggaagaaat tcgtgccgtg gaacagaacg
      961 tcaccgaaaa aatccgcagc aatttcacca ttgaggaaca ccgccacgta cccatcgatg
     1021 aggcgagagc acaaggtgcc atggcccttt tcggcgagaa atacggcgac gatgttcgca
     1081 cggtccgttt tgggtcgtcc attgaacttt gcggaggaac acatatctct tccaccgggc
     1141 gtatcggcac attccgtatc gtaagtgaaa gcgccatcgc tgccggtgtc cgccgcatag
     1201 aagccgttac tgccaaaggc tgcgaagatt atttgtacac gatggaagac atgctccgaa
     1261 acataaaaga agcattgtcg ggtaacgcgc ccgaaatcat ggtgggctta cgcaaactga
     1321 tgagcgaaaa cgaggagatg cggcaaaaaa ttgaagcgtt tacaaaagag cgcaccgtac
     1381 agttaaagaa cctcattatc cagaaaaaag agatcgttaa cggtgtcacg gtgtttaagc
     1441 ttgaaggtat ggtggttacc cccgaaattg ttaaggatat tgcctttcag ctgagaggag
     1501 agtttccgga atacatggcc tttatggcgg caaccggtgg caacaacaaa ccaagcctga
     1561 cagtaatgct gagcgacgac ttagtgtcag gagggcttaa tgcaggaacc attgtacgtg
     1621 aagcagccaa acatattcag ggaggaggag gaggccaacc acactttgcg actgccggag
     1681 gtaaaaatgc cgatgggtta aagatggcga tggaaacaat gttgcaatat ttaccataag
     1741 ccggaaaaaa ctcccgggtt ttgttgttta ggtccctttt tttcatatat ttgagacatt
     1801 taaaaacctc aacaatatgg acaagcgaca aattaccctg aaagacattg ccaaggagtt
     1861 aaaattatca ccctcaaccg tatcccgggc actaaggaat catccggcca tcagcaagga
     1921 aaccaaagag cttgtacggg actatgccgt gaaacacaaa tacaagccta acacgcttgc
     1981 gcttcaactt cgtacgaatc ggaacaatac cattggcgtt attgttcctg aaattgtgca
     2041 ctattttttt tctacggtaa tagccggtat tcaggatgaa gcggaaaaag agaattataa
     2101 cctgatcata tgccagtcca acgaaaatta cggccgggaa gtaaagagcg tggaaaccct
     2161 tcttgatgcg agggtacggg gcattctggc atctcaatcc aaatcgaccg aagagttttc
     2221 tcacttccag gacattatag acaacgggac acatctggta tttttcgacc gtatctgtac
     2281 cgggatcaat accgacaagg tggttataga cgactacgcc ggcgcttata atgccgttga
     2341 ttatctaatc ggaagcggat gcacacgcat tgcttttctt ggctccaacc cacacctgcc
     2401 cattgccaac aacagaagga tgggttacga agatgcgttg cggaatcata atatccccgt
     2461 ggataaatcc cttatgcagg tatgcgacac cattgagctt gcccgtgaaa cagtgcctgt
     2521 catgctgaag attactcctg ttcccgatgc ttttttctgt atcaacgacg aggtggcgtc
     2581 ttattgtctt cagatctgca agtcagcagg atacaagatt ccacaggaca tatcggtttg
     2641 tggatttacc aattcataca ttactgaagt gaccgatcct acacttacca gcgtagacca
     2701 gcacggtttc gagatgggaa aaactgcagc acgcctgctt ataaaccgca taaaaggtat
     2761 cgagaagaag acaggcatcg tgagcaaagt cattaaaact aagctggtgg tcaggaactc
     2821 cacaagaaat tgataccgaa tcgcccctca gtcattgcgt cactggctcc aatgcattcg
     2881 attgcatacg ttttttcact tttttacttt aaatacagct ttctgatgtc caaagcatta
     2941 taaatttgtt tcttaacaga agaaaaacat cacaacatga agaaaagaca cctctctttt
     3001 tgggaaatct ggaacatgag tttcggattt ctgggaattc aattcggttt tgcacttcag
     3061 aacgcaaatg tcagtcgcat tttcgagaca ctgggcgcac gggtggaaga tatccccata
     3121 ctctggattg ccgccccgct taccgggctc atcgttcagc ccatcatcgg gcacctgagc
     3181 gataaaacct ggaaccggtt cggccggcgc cgcccttatt ttaccatcgg tgccattttt
     3241 actacgctgg cgttgttcgt catgcctaat tctccggcac tgtggattgc agccggcatg
     3301 ttatggatta tggatgcctc catcaatatc tccatggagc cgttccgtgc ttttgtagga
     3361 gacaatcttc cttctgaaca gcggacaatg gggtttgcca tgcagagctt ttttatcggt
     3421 ttgggtgccg ttgttgcttc ggcattgcca tacatcttaa ccaactggtt tggtatcccc
     3481 aatactgccg gtgagggcat aataccccag tcggttaaac tctcgttcta tatcggaggg
     3541 gttgttctac tggcatcggt cctgtacacc gtttttactt ccaaagaata ttctcctgaa
     3601 gaattggccg cttttgaaca ggaaaaggaa aaaatatccg gcattaaaac cgagataaag
     3661 ccaccggtca ctccggttca atttcttaaa aacggttccg tctggacaac tgcaggaatc
     3721 attgcaactg caataattgt tttattgaag ctcgaagctc aactctatat tgtttcgggg
     3781 ttggtattga cctttggaat tattttactg gtatcgggca tgctgactaa aaagggaagg
     3841 acccaaaacg gcatggtggg catcatgaac gacttgcttc atatgccaag aaccatgggg
     3901 caactggccg tggtccagtt tttctcgtgg ttgtcgtttt acgccatgtg gatttatact
     3961 acgccggccg taacccaaca tgtctttggg acgaatgaca cagcttccga gctctacaat
     4021 cagggtgccg actgggtagg ggtccttttt gccgtttaca atgccgtgtc ggccctgtcg
     4081 gcgtttctgc ttccggttct tgcccgtcag atcggacgga aggcaaccca ttccattgca
     4141 ctggcattgg gcgggatctc atttatctcg ttcttcttta ttcagaatcc ccaaattctt
     4201 ctattgccta tgattggtat cggtttcacc tggggaagca tcctatccat gccgtatgcc
     4261 attctcacgg gctcccttcc ggcagataaa atgggaacat atatggggat tttcaatttt
     4321 tttatcgtta ttccgcaaat cctggctgcc agcatcctgg gattcgtcac gaaagatatt
     4381 tttggaggac aagtaatttt cacgatggtt cttgccggat gttcacttgt tctcggagct
     4441 ttatcggttt tcttcgttca agatcgggac gatgcgtata aatttaaaaa aaatgcacaa
     4501 gaacaaaaat aacaatttaa gtttcgcatg tgttgctaag gagcttgaat aaataacaca
     4561 gcaatgaaaa aaacaatttt ccttttatgg attgtgtcgc ttctgttttc gtgcggaaac
     4621 aaaaagcagg aagcggcaac aagtgtacac cccgaatggg tttacgatgc cactatctac
     4681 gaggtaaaca cgcgacaatt cacgccagag ggtacgttca atacctttgc cgctcattta
     4741 ccccggctga aagaactggg cgtggacctg ttgtggttca tgcctgttca acctatcgga
     4801 gaaaaggaca ggaaaggaac attggggagt tattactcca tcaggaacta tacggaggta
     4861 aaccctgaat tcggcaccat ggacgatttt ataaccgtgg taaaagaagc acacgacatg
     4921 ggtatgaaag taattctgga ctgggtaccc aaccatacct cacgcgatca cacgtggata
     4981 acggagcact ccgactggta caagagggac agtttgggaa gtccgattat catgtacgac
     5041 tggacagata tcgcgccact ggattacggc aagaacgaaa tgcgcaaggc gatgattgac
     5101 gccatggcgt tttgggtgcg tgaggccgat atcgacggtt tccgctgtga tgtagcctat
     5161 gaagtcccga ttgatttttg ggaaacggca aaggattcac tcataacgct gaaaccggat
     5221 attttcatgc ttgccgaagc tgaaaaaccg gaactcaacg aatcaatttt tgatgcttat
     5281 tatgcgtggg atctccacca caaaatgaat atggtggctc agggaaaaga aaacgtggat
     5341 tctctacggc tatcgctgaa gcggatgaac gaacgttttt cctcccatgc cataccgatg
     5401 tatttcacct cgaaccacga tgaaaactcg tggaacggaa cggagtttga acgaatgggc
     5461 gaggccgccg gaacctttgc cgtactcacc tatatgcttc ccggcatgcc tctgatttac
     5521 agcggacaag aggccggatt aaaccgcagg ctgcagtttt ttgaaaaaga cagtatcaat
     5581 tggaccgaca ggggcggatt taccggttta tacacatcgc ttaacgcatt aaaaaaaacc
     5641 aataaagcgt tgctttcacc ggagagaggg ggaaagatga ctgaaatcgt gaacgacagt
     5701 ccggcaagcg ttttctcttt caaacgcgcc catgaaggaa acgagatcgt ttgtgttttt
     5761 aatctcagca aatacagtgt gaaaactgtt ttcaacgaag aagtgccagg tgcaggcttt
     5821 accgctttcc cttctgccga aacagtaacg cctgtaaaaa aaatggaact ggccccctgg
     5881 gaataccgga tatatgtcaa atagccggta ataactttac aaccacttcc ttttcttgaa
     5941 atacaacagg atggccagcg atgaaagcac catcaaccct atcgatacgg gatatcccca
     6001 tttcatgttc agctctggca tggctgtaaa attcatcccg taaatactgg cgataagcgt
     6061 gggcggcata aaaatcaccg acacaatggt aaagatcttg atgattttgt tctgttcgat
     6121 attaacataa cctaaaaacg tgtcctgcaa gtagtccaac cggtcgaaac tgaacttgat
     6181 gtgctccacc agcgagttga tatcccggat caccatcgat agtttcggct gtagttcctt
     6241 gggaataaaa tcactgcgta acatgctcga aaccgtgcgt tgcttatcga taatgttctc
     6301 gcggagcatc atggtctttt cctgtaagtt cttgatctcg cccagcaact ccttgtcggc
     6361 ttcctgcaga ctcaagctgt tactgagctg ggtgatcttg tgtgtcatgt tttcaatcat
     6421 atcggcatca aactccaccc gggtttcgag caatgccacc agaacatctg caccggtatt
     6481 gtatcctttg gggtttgccg atattttctt caccgtttca tgaaacgaaa taagttcctc
     6541 gctgcgaacc gtaaccaata tattgttttt gagtataaac gacaccgggt cgctttcgaa
     6601 gttcgacaaa aggaagttcg agttaaccac cagtgtatcc tgcgtctcga tgtaacgcga
     6661 cgacatctcg atctcgatca tttcctcttc ttcctgaatg taaatcttca gaaagtcttc
     6721 gagttcgttt tccacttctt cgtcaacgtc attaaggtct atccatagaa agctctggat
     6781 agggtttgat ttgagaaaat caaggctgcg gctgagtcta accacgccgt ctttgtggta
     6841 aaagagtttg atttccgcca tgatgtttcg gtttttaatc gatgaataat cagttcttgt
     6901 tttcttccaa gaaatttttg ccggctaagg gttcgagcag gttcgtcagc tgttcgaatt
     6961 gttcgatcga cagctgctcg ggacgttttg tgagcatcgg gtcgtcgggc aaaggacttt
     7021 cttcgggcag cagcgatctg atggagtttc tcagcatttt ccttcgctgg ttgaaacttg
     7081 ttttcactac cgacttgaac agtttttcat tgcagttgag ctgctttcgt ttattacgtg
     7141 taagccgcac aactgccgat ttgaccttgg gcggaggaat aaacgcctgt tcgctcaccg
     7201 tgaagaggta ctcgatatcg taccaggcct gcagcaaaac actgagtatg ccgtaagctt
     7261 tactaccggg tgaagcaatc atgcgttcag ccacctcctt ctgtatcata cccgcacaac
     7321 agggaatcag gtccttgttt tcgagcactt taaagaaaat ttgcgtggat atattgtaag
     7381 gatagttacc aataacacag aatggtccgt cgtaatattc cgcaacatcc agggtcagga
     7441 aattttgctg caaaatattt ccgttcaacg aaggaaaata ctccttcagg taagcaatgg
     7501 actcccggtc gatttccaca accgtaaggt ttctgccttt tgacagcaga aattgcgtga
     7561 gcataccggt acccggccct acctcgagaa tgggaagcgg agcaaaagcg tcgagcgtgt
     7621 cggcaatccg actggcaatt gctgaatctt tcaggaaatg ttgcccgagg ttttttttag
     7681 gttttaccga tatcattgtc aattagcgat tattagctat ctttacggtc gcaaaggtaa
     7741 ggaataattt aaaaattttg gcaatcaacg gtcaaaaaag gatagaacaa taagttaaaa
     7801 tagtgctttt tagctttctt tttatgcctt tcgctcctac tctagaaaga ccctattcag
     7861 ttaagatgaa ctcaaagcgc atacaggata ttctaaaaac agtattatca cttgcacttg
     7921 gcgtagctat aatctggctg ctctatcgca aaaccaattt aagagaagtg tgggagattg
     7981 caagctccgc tcatttcggg attatcgctt catcgctcgt gttcgggctt ttgggcaact
     8041 attttcgcgc attacgctgg gaacttttcc tgaattccct gggataccga cccaagcggg
     8101 agagcattgt gttcgccacc tttggaaatt atgccgtcaa ttttcttttg ccccgcgctg
     8161 gagacctgtg gcgatgtggg atagtaacca aatacgatca gatccctttc tccaaaactt
     8221 tcgaaacttt ttttgtagat aaaatcctcg atatcttggc gggtgttgtt ctcgttatca
     8281 tcagcctggc gctttatgtc gacttctttg tatcttattt tcatcaaaac cccggctttg
     8341 cagaaaactt cacgaaatta tttacgtccg tttggctgta cctgcttctc atactgatta
     8401 gcggcggaat ctttgtcatg tttttctact ttaaagaaaa ccctctggta aaaaaagtca
     8461 acaagttcct acaggtcgtc cagtacgaca tggtactgat tgccaagatg aaatcgaaag
     8521 ggaaaataat tatttacacc gttctcgcct ggttgagttt ttatgtgtat ttttacattt
     8581 gcttttatgc ctttgatttc accaaaaacc tgggtccgat aatcgggtgg attgtatacg
     8641 ccatgagtaa cgtcggcgtg gcagtacccg ttcagggagg cgtcgggaca tggcatttca
     8701 tggtaatctc ctccctggtt atcttcggga taggttatga ggaggccagc gcatttgcag
     8761 gagcgatctt caccgtccaa tcggtttgga tcatcctttt gggagtgatg ggaattctag
     8821 ctcttccgta tgtaaaaaga gaaaaagaga atttaggatt gcccgttatc gaaaatcaga
     8881 aacaataaat atataattat gtcaacatca gaaattttaa acttaaatcc ccaaggggtc
     8941 tggaaatatt ttcacgaact caatcaaatt ccacgcccta ccggacagat ggaagaggtc
     9001 acgaaatttg taatgaattt tggcaaatcg ctcaacctgg aaaccaggca ggataaagca
     9061 gggaatgtgc tcataaaaaa gccggcatca aaagggtacg aaagcggaaa aaccattatc
     9121 attcaatcgc acctggatat ggttccccaa aaaaacgctg atgttaccca cgatttctcc
     9181 agagacccca ttcaaacgta tatcgacggt gagtgggtga aagcccgctc cacaacgttg
     9241 ggagcagaca acggtatcgg ctgcgccatg atgatggccg tactggaaga taacctgctg
     9301 aatcacccgc ctatagaagc tcttttcact gtggatgaag aagtgggaat ggacggtgcc
     9361 tttggtctgc agaaaggctt tctgagcggc acggtcatgt tgaatttaga taccgaagaa
     9421 gacggcgatc tctgtgttgg ttgtgccgga gggacagacg tgaatgtctc tttccagttt
     9481 aagcccgatg aagaaattga gaaaggcgac gtggctttta agataagcct taccgggctc
     9541 aaaggcggac actcgggaac acagatccat ctgggacatg cgaatgccaa caaactgatg
     9601 aaccggtttc tcaaagacgt tgtgagaaac tacgaagccc gtctcgcaaa cattcacgga
     9661 ggatcactga gaaacgccat tccacgcgaa tcatttgccg ttgtcaccat ccccgaacaa
     9721 ctttcggagg acctggttga tttggtgaat gaatacgaat cgcttttccg ggaagaattt
     9781 tcaggaattg aaagctccat ctcttttaaa gctgaaaaaa ccgacgtgcc ggaatcgttg
     9841 ttaccgattg aggttcagga tgatctgatc aatgccgtag aaggttgtcc aaacggagta
     9901 atcagtatgc ttgccgaatt tccgggtgta gtagaaagct cgctcaacct ggccctggtt
     9961 cagtcgacgg aaggacagat tgaggtgaaa ctgctggtcc gaagctcctc ggaaagccgg
    10021 aaagaatggg tttgttcatc ggtggaaagc gtgtttatgc ttgccggagc aaaagtggaa
    10081 ttcgacggtg cttatcccgg ctggcaaccc gatgccaact cagaactact gaccacgatg
    10141 acgaaaatat acatggagaa gtacggtcaa cgtcccaacg taaatgttat tcacgccgga
    10201 ctggaatgcg gaatcattca ggcaaatgcc ggacacaaac ttgatatcgt ttcattcggc
    10261 cccaccatca ccggtgcaca ttcgcccgat gaagccgttc atatcgcagc cgtcggaaaa
    10321 tcctacgact acctgttagc tattttggaa caaatgaaat aaatttctat tctgcacgca
    10381 cttcttccca aaacaaccat cagatggcag caaacaacac actcattgtt tatgcttccc
    10441 ggcgcggaga cgaggagaag tgcgcgcggg agattttcaa tttgatcgac ggtaaagtag
    10501 atatctgcga cctgagtaac agagaatctt ttcccgatgc gtcaacgtat gatactgtca
    10561 ttataggcag ttccgtttat cacggaaaag ttttggagtt ggtctctaat ttttgccgga
    10621 taaacctcga catacttacc cggaagcgtc tcgggctttt catcatatgt ccggattccg
    10681 gcgaaagggc agaaaaagaa ttgcaggaag cttatccacc cgatcttttt aacagtgcca
    10741 ttgccaggga ctacttcggt ggagagatcg atccttacag gctgtccatc ctggaaaaaa
    10801 taataacgta ccggctgtcc ggaccaagaa aaccgtcgat ccctgcctcc cgggaaaaaa
    10861 ttaaaaaatt tgccaaaaaa atgaacccgt cccatgcctc tcaagagtaa aaggtacata
    10921 tttctaccgc tggctttagc tgcttatgct a
//
LOCUS       DAOSDR010000044        10935 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrimonas sp. isolate AMR_MDS_1826
            NODE_2306_length_10935_cov_3.820404, whole genome shotgun sequence.
ACCESSION   DAOSDR010000044 DAOSDR010000000
VERSION     DAOSDR010000044.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863355
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrimonas sp. (wastewater metagenome)
  ORGANISM  Petrimonas sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Dysgonomonadaceae; Petrimonas.
REFERENCE   1  (bases 1 to 10935)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10935)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:10:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,720
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 35
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10935
                     /organism="Petrimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2306_length_10935_cov_3.820404"
                     /isolate="AMR_MDS_1826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2023866"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..650
                     /locus_tag="PLI30_02935"
     CDS             <1..650
                     /locus_tag="PLI30_02935"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOI78541.1"
                     /translation="KTEIELLGVEVLALSVNMASAEDNQRIIDETIRKFGRIDILVNN
                     SGGPKPGTFREVTSDDLDDAYRSVLKYNIRMIQGCLPFMERNGWGRIVNITSITVKEP
                     APNMVLSNIFRSSVVSFAKTISKELVSKGITINNVSPGYFKTDRVMQLMKVRSEAEGI
                     STNEYEQRAILDFPHKRYMDPEELGNLVCYLCSEQARSINGTTIQVDGGMLNGLL"
     gene            complement(679..1197)
                     /locus_tag="PLI30_02940"
     CDS             complement(679..1197)
                     /locus_tag="PLI30_02940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI78542.1"
                     /translation="MNNRDLHFPEEGFNRPLFLPNDKLEVVSFRQGVRKRKSRTASVI
                     AWIQLVGWLIYFAINLINHLRFNLSETPAKNSLLSQTLSAFVYEFSQITFIWVTISSI
                     LSIVGLHKMKSWGWTAAIAANSLWLSIFAFTFTQNPSERTVFEKTFFIVFALFAVVST
                     LCLWRKRFVFWR"
     gene            complement(1336..2451)
                     /locus_tag="PLI30_02945"
     CDS             complement(1336..2451)
                     /locus_tag="PLI30_02945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007660485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOI78543.1"
                     /translation="MIKSIAKLILALSISTLPVVTPAQQKPVRIGIAGLSHDHIHGLL
                     GRKEKGDIVIVGIAEPDSGLVERLAKRYGFDKKIVYSSIDEMLRSTKPEAVMAYNDIF
                     GHLEVVEKCAPRGIHVMVEKPLAVSVAHADKMAKLAKKNNIQILTNYETTWYGSNTEA
                     YKVVNEEEQIGEILKIEFHTGHQGPVEIGCSKEFLAWLTDPVLNGAGALNDFGCYGAN
                     LSTWLMKGETPVSVTAVTHTNKPQVYPNVDDEASIIVQYPYNQVIIQASWNWPYNRKE
                     MKIYGQSGYVFCRDTENMTVFKSKEKKATDKAAPALNEDRNDAFSYFARVVRGDINPQ
                     PYDLSALPNNEVVVKILEMAKKSAENGKTIVWKEYFK"
     gene            complement(2474..3364)
                     /locus_tag="PLI30_02950"
     CDS             complement(2474..3364)
                     /locus_tag="PLI30_02950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019540166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HOI78544.1"
                     /translation="MKYGKVEDPTGIDFRIPADDKATRGVFKNVPESGVEAYIGCAKW
                     NKTDLKGFYPKGTKDELAYYSRQFNSIEMNSIFYNMPKAEQVVKWKNKTPDGFKFFPK
                     VTQSISHMRRLDNVDELTAIYCDAISHFEEKLGMCFLQLHENFGIKELKKLKVFVENF
                     PKVIPLAVEVRGLEWFSDKVTWNGFCDFLEEHKTTNIIVDTAGRRDMLHMRLTTPTAF
                     IRFVGCNVDEIDRKRIDDWVDRIEKWRKEGLQHLYFFVHQNVEVSSPLFSAYLTEKLN
                     ERLNLHLHVPQMAGEQGKLF"
     gene            complement(3369..4289)
                     /locus_tag="PLI30_02955"
     CDS             complement(3369..4289)
                     /locus_tag="PLI30_02955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008773214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HOI78545.1"
                     /translation="MKYFYLLFLCTICSFSVHSQVLKNLESELVLLNVKTGKEKVILR
                     EKRHFEAPNWSRDGKFLIINSDGKLEKVSVKGEKLGIIDTGFADRCNNDHGLSYDGKW
                     LIISHNDSRFTSPGGSSRIFILPVSGGIPRLVTANYPSYWHGISPDNQWITYCAMRNG
                     QWDVYKTHVITDKEVRLTDTDGLDDGPEYSYDGRWIYFNSHRTGRMHIYRMCPDGSNQ
                     TQLTFDEYDNWFPHPGPDNKSIAYIAYIEDQKGGHPFGKNVKLRLLDTESKIIRDLTP
                     VFYGGQGTINVHSWSPDGSRIAFVRYTDND"
     gene            complement(4306..5271)
                     /gene="hemH"
                     /locus_tag="PLI30_02960"
     CDS             complement(4306..5271)
                     /gene="hemH"
                     /locus_tag="PLI30_02960"
                     /inference="COORDINATES: protein motif:HMM:NF012964.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004325 - ferrochelatase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HOI78546.1"
                     /translation="MRGILLINIGTPASCSKEAVRKFVGDMLSDPLVTGQPEWVSSFL
                     AKKIIAPVSSGKSFNKYSQIWRKEDPEISPMIYFMQKLAQSLEAKKNIPVEVAMRYGE
                     PEIEQALKDLEKKCPLLHEVVVFPLYPHYAQSTTQTTIDEIGRVFYKRPHSYRLQLVE
                     PYFDHPAFINALAKHAEPYLKDIDKLVFSYHSLPVDQVEAGWKKGREFDYVYQLKETN
                     RLFCEKLNIQLQYTLLLYASQRGNNWLKPFLDKDISDLPRLGWKKVAVIAPGFPVDNL
                     ETLYDIDIEARELFMKAGGEKFVFVPSLNNSDEWIEAIWKITVGV"
     gene            complement(5305..6240)
                     /locus_tag="PLI30_02965"
     CDS             complement(5305..6240)
                     /locus_tag="PLI30_02965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019541093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase"
                     /protein_id="HOI78547.1"
                     /translation="MIWGGSDICKFKNITPAEDGIGESWEISGVEGKISIVGNGELAG
                     KPIDEVLRTSKARLVGKKVYEKFGNTFPLLIKFIDARDDLSIQVHPDDKLAKERHNSF
                     GKTEMWYVIKASSDAFLYSGFSTQITSDEYIKTIENNSFTDKLQKYDVKTGDVFFLPA
                     GRVHAIGAGCFIAEIQQTSDLTYRIYDYNRKDAQGNLRELHTELAKDAIDYNVYGNYQ
                     TEYTNRLNQPVELVTCEYFSTNLLEMDIPLARHYNALDSFVIYICMQGHCTLKDNNGN
                     SVFIGQGETVLVPAETESVLIIPSETTKILETFIK"
     gene            complement(6457..8463)
                     /locus_tag="PLI30_02970"
     CDS             complement(6457..8463)
                     /locus_tag="PLI30_02970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008774197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha amylase C-terminal domain-containing
                     protein"
                     /protein_id="HOI78548.1"
                     /translation="MLDIVKNDPWLEPFSRSIEDRNNYFLKKEKELTQNGKIRLSDFA
                     TGYLYFGLHKTTDGWVFREWAPNATEIFLIGTFNNWQRMEPYRLRSIEKGVWEVKIPP
                     EHIRHQDLYKLLVSWEGGSGERIPAWCRRVVQDAQTHIFSAQVWEPATPYTFKITNFK
                     PDTSPLLIYECHVGMATAEEKVGTYNEFRENVLPRIKNSGYNAIQLMAIQEHPYYGSF
                     GYHVSNFFAPTSRFGTPDELRQLIDAAHEMGIAVIMDIVHSHVVKNELEGIARFDGSY
                     DLYFPSNPNRRNHPAWDSLCFDYGKNEVLHFLLSNCKYWMEEYRFDGLRFDGVTSMLY
                     YSHGLEESFTRYDDYYNNRQDLDAICYLTLANKLIHEVNPTAITIAEEVSGMPGLGLD
                     IKSGGYGFDYRMAMNIPDFWIKTIKERKDEEWHPSAIWWETTNRRADEKTISYVESHD
                     QALVGDKTIIFRLIDADMYWFMSKLKSSSFRVDRGMALHKMIRLVTATTINGGYLNFM
                     GNEFGHPEWIDFPREGNNWSHKYARRQWQLADDKNLKYHYLGDFDKAMINLIKSVPDF
                     QTTTITKIWDKDDDNVLAYMRERLLFVFNFHPEKSFNDYGMLVPEGEYSIVLNTDNFR
                     FGGFGLNNDTVHHFTHHDPLYAKDGKGWIKIYLPARSAFVLKKI"
     gene            complement(8488..8937)
                     /locus_tag="PLI30_02975"
     CDS             complement(8488..8937)
                     /locus_tag="PLI30_02975"
                     /inference="COORDINATES: protein motif:HMM:TIGR00022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HOI78549.1"
                     /translation="MILDNIENAPNYFGLNPLFEKAFEYVRGLDVDNLPEGTSEIEGV
                     YLKASAVKIALKSKTETKLETHRKFIDIQIPITREEIFGWKSLKNLKMPTQEYDSQND
                     FQLFEDEPSTYIRVLPGEFIIFSPEDAHAPLIGEGITRKIILKVAVL"
     gene            9078..10511
                     /locus_tag="PLI30_02980"
     CDS             9078..10511
                     /locus_tag="PLI30_02980"
                     /inference="COORDINATES: protein motif:HMM:NF024978.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HOI78550.1"
                     /translation="MKRILPAFIILLISVAVFSQNDQTTCNLGFSFKISNNSNWGNNE
                     PVVTEVVPGSPAEKAGLKANDIILEVNGNGTYLKPSHTIMSWFMEKPSEMSISIRNFE
                     ASFKPMHIAKDCRPRNGLSEAQLAPVFSFYSLEDIQDRKFIIPVKTTINPDADFFNYR
                     TYDFAPSDVSSREMDERINSIFVRVLSQLGLKRDSEDPDFIIQTFYSYQNNPMFKTES
                     PTRGTYSGTWRFDTRNNRMVKIPVFDPTQPVRIDDVMYDLEFGYRFYDRKFTEPGRSM
                     LVWESEVKEKLSDNYGLLDYLEMNLPLILSKFPNSGNLERATYHVKYLRYNYTGISYD
                     LNDLKTVVSVDAGSPAARAGIKPGDVVIKVQGHNFNHDAASLTSSYRRFIAETMKYRD
                     PATKYTDSNGFQNAMYWDIIHYNSISKEINDKKRYKAGFSYLFNFNQYIDWDTPDTLN
                     IDVERKGEKLSFEVKPIINRHSHVSVE"
BASE COUNT         2921 a         2352 c         2359 g         3303 t
ORIGIN      
        1 ccaaaactga aatagagttg ctgggagttg aggtgttggc tttgtcggtt aacatggctt
       61 ctgctgagga taatcagcgg attatcgatg aaaccattcg gaaatttggc agaatcgata
      121 ttttggtcaa caactccggt ggcccgaagc cgggaacctt tcgggaggtt acttcagatg
      181 atttggatga cgcataccgt tcggtgctga aatacaacat ccggatgatt cagggttgcc
      241 tccctttcat ggaaaggaac gggtggggca gaatcgtgaa catcacttcc atcactgtga
      301 aggagcccgc tcctaacatg gtgttgtcga atattttccg ctcatcggta gtgagttttg
      361 ccaaaacgat cagtaaagag cttgtctcaa agggtataac catcaataac gtttctcccg
      421 gttatttcaa gaccgacagg gttatgcaac tgatgaaagt ccgctccgaa gcggaaggga
      481 tttccacaaa tgagtatgaa cagagagcta tcctggactt tccacacaaa cgctatatgg
      541 atccggagga actgggcaat ttggtttgtt atctgtgttc ggaacaggcc aggtcgatca
      601 acggaacaac cattcaggta gacggtggta tgttgaatgg gttgttgtga ggtgaaacga
      661 gaaaaaagct taccgggatt atctccaaaa gacaaatcgt ttccgccaca ggcataatgt
      721 ggaaactacg gcaaacaggg cgaaaacgat gaagaacgtt ttttcaaaaa ctgttctttc
      781 agacgggttt tgagtaaatg taaacgcaaa tatggatagc cagagagagt ttgctgcaat
      841 cgcggcagtc caaccccagg atttcatttt atgtaatccc acaatactta aaatggagga
      901 tatggtcacc cagatgaaag tgatttgaga gaactcgtaa acgaatgcgg atagtgtttg
      961 agagagaagt gagttcttcg cgggagtttc ggacaggttg aagcgaaggt gatttataag
     1021 gttgatcgca aaatagatca accatcctac caattgaatc caggcgatta cggaggccgt
     1081 tctcgatttt ctcttccgca ccccttgccg aaaagacacg acttctaatt tatcgttagg
     1141 taaaaacaac gggcgattga aaccctcctc tggaaaatgt aaatcacgat tgttcattcg
     1201 agtgtctatt acttttcacc acaaaaataa agaataaccg taaatggaga taaaaaaacg
     1261 gttacgtttt tatgaagttt ttttcgccgg tatagtcact tcgtaaaata aatgaattgt
     1321 agcgaaatat aggatttatt tgaaatattc tttccacaca attgtttttc cgttttcagc
     1381 agattttttc gccatttcca gaattttaac cactacctca ttattgggca gggctgaaag
     1441 atcatacggt tgagggttaa tatctccgcg gacgacacgg gcaaaataag aaaaagcgtc
     1501 gtttctgtct tcgtttaatg ccggggcggc tttgtctgtg gccttttttt cttttgattt
     1561 aaatacagtc atattttcgg tatctctaca gaaaacatat cccgattgtc cgtaaatctt
     1621 catctctttc cggttgtaag gccagttcca tgatgcctgg ataatcactt gattatacgg
     1681 atactgtacg atgatgctgg cctcatcatc cacgtttgga taaacctgtg gtttattggt
     1741 gtgcgtcacg gccgtaacag aaacgggcgt ttcgcctttc ataagccaag tggaaaggtt
     1801 ggctccgtaa cagccaaaat cgttgagcgc tccggcacca tttagtacag gatcggtaag
     1861 ccaggcgagg aactctttgc tgcagccaat ctctactggt ccctgatgtc cggtatgaaa
     1921 ttcaattttg aggatttccc cgatttgttc ttcctcgttc acaactttat atgcttcggt
     1981 gttgcttccg taccaggttg tttcatagtt ggtaagtatt tgtatattat tttttttggc
     2041 gagtttggcc attttatcgg catgtgccac actcacggcg agtggttttt caaccataac
     2101 gtgaatacct ctcggggcgc atttctcgac tacttccagg tgcccgaaaa tatcgttgta
     2161 agccattact gcttcgggtt ttgtgctgcg gagcatttca tcgatactcg aatagactat
     2221 ttttttgtcg aaaccgtacc ttttcgctaa tctttccacc agtccgctgt ccggttccgc
     2281 gataccaacg ataacaatat cgcctttctc ttttctcccc aggagcccgt gaatgtgatc
     2341 gtgtgacaag cccgcgatac caattcttac tggtttttgc tgtgctggcg tgacaacagg
     2401 tagtgtgctt atacttaatg cgagtataag ctttgcaatg gatttaatca tgatcattga
     2461 atatctgttt tttttaaaat aatttcccct gttctcctgc catttgggga acatgtaagt
     2521 ggaggttaag cctttcgttc agtttttctg tcaggtacgc agaaaaaaga ggcgaagaca
     2581 cttctacatt ttgatggacg aagaagtaaa gatgttgtaa tccttccttg cgccactttt
     2641 caatacggtc aacccagtcg tcgatacgct tgcggtcaat ttcatcgaca ttgcaaccca
     2701 cgaaacggat aaaggccgtg ggagtagtca acctcatatg cagcatgtcg cgccggccgg
     2761 cagtatctac aatgatattg gtggtcttat gctcttccag aaaatcgcaa aaaccgttcc
     2821 aggttacctt atcggaaaac cattccagac cgcgcacttc caccgccagt gggatcactt
     2881 tcgggaaatt ttcaacgaaa accttcaatt ttttcaactc tttgatgccg aaattttcgt
     2941 gcagttgcag aaaacacatt ccgagctttt cttcaaagtg tgaaatggca tcgcaataga
     3001 tggccgttaa ttcgtctaca ttgtccaacc ggcgcatgtg gctgatggat tgcgtcactt
     3061 tcggaaaaaa cttgaatccg tccggagttt tgtttttcca tttcaccacc tgttctgctt
     3121 tcggcatatt ataaaatatg gagttcatct caatggagtt aaactgtctt gaatagtagg
     3181 ccagttcgtc ttttgtgcct tttgggtaaa aacctttcag gtcagttttg ttccatttcg
     3241 cacaaccaat atacgcttcg accccgcttt ccggtacgtt tttaaaaacg ccccgggttg
     3301 ctttgtcatc ggcgggaatt ctaaaatcta tgcccgtcgg gtcctctact tttccgtatt
     3361 tcatgttgtc aatcgttatc ggtatatctt acaaaagcaa ttctgctgcc atcgggtgac
     3421 cagctatgca cattgatcgt tccctgcccg ccgtaaaaaa cgggagtcag gtcgcggata
     3481 atttttgatt ctgtgtccag taaacgaagc tttacgttct tgccaaacgg atgcccccct
     3541 ttctggtctt cgatataggc gatataagcg atgcttttat tgtcgggtcc gggatgcggg
     3601 aaccagttgt cgtactcatc gaaagtgagt tgggtttggt tacttccgtc ggggcacatc
     3661 cggtagatat gcattcgtcc ggtgcggtgg ctgttgaaat agatccatcg gccgtcgtag
     3721 ctgtattccg ggccgtcgtc tagtccatcc gtgtctgtca acctgacttc tttatcggtg
     3781 ataacgtggg ttttgtaaac atcccattgg ccgtttcgca tggcacaata ggtaatccac
     3841 tgattgtcgg ggctgattcc gtgccagtaa ctcggataat tcgcggtgac taatcgggga
     3901 ataccgccgg atacgggaag gataaagata cgggaacttc ctcccggcga ggtgaatctt
     3961 gaatcgttat ggcttataat gagccatttc ccgtcgtaag aaagtccatg atcgttgtta
     4021 caccggtcgg caaatccggt atcgataatg ccaagctttt cacctttaac cgatactttt
     4081 tctaattttc catcggagtt gatgatcaga aatttcccat cacgcgacca gttgggtgct
     4141 tcaaaatggc gtttttcacg caggatgact ttctcctttc cggtttttac gttgagtaag
     4201 accaattcgc tctccaggtt ctttaaaacc tgtgaatgta ctgaaaatga gcagatcgtg
     4261 cacagaaaaa gcaaataaaa atatttcatt gtttttaatt tgtgcttaaa ctcctacggt
     4321 tattttccaa atcgcttcta tccactcatc ggagttattg agggagggaa caaagacaaa
     4381 tttttctcct ccggctttca taaacaattc gcgagcctca atgtcgatgt cgtacagtgt
     4441 ttccagatta tccaccggaa acccaggggc tattaccgct acttttttcc atcctagccg
     4501 gggtaaatcg gaaatatctt tatctaaaaa aggtttcagc caattgttgc cgcgttgtga
     4561 tgcataaaga agaagcgtat attgcagttg aatgttgagc ttttcgcaga ataaccgatt
     4621 ggtttctttc aattgataga cataatcaaa ctctctccct ttcttccagc ctgcctccac
     4681 ctggtcaacc ggcagactgt gatagctaaa caccaacttg tcgatatctt tcaggtaagg
     4741 ctccgcatgt ttggcaagtg catttataaa tgccggatgg tcgaaataag gctcaacaag
     4801 ctgcagccgg tacgagtgcg gacgtttgta aaaaacccgt ccgatttcat ctatcgtcgt
     4861 ttgcgtggta gattgggcat aatgcggata caagggaaat acaacaactt cgtgcaacag
     4921 tggacatttt ttctccaggt ctttcaatgc ttgttcaatc tccggttcac cgtatcgcat
     4981 ggcgacttcc accggaatat tttttttcgc ctccagtgac tgggctagtt tttgcatgaa
     5041 atagatcatg ggagagatct cgggatcttc ttttcgccag atttgactgt atttattaaa
     5101 cgacttaccg gatgaaaccg gagcgataat cttctttgcc aaaaagctgg aaacccattc
     5161 aggctggccg gtaacaagcg gatcgctaag catatcgccc acaaatttcc taacagcttc
     5221 tttactgcag ctagccggag ttccaatatt tatcaataaa ataccgcgca taaacgtgtt
     5281 acttgttaga ctttatacgt gaagctattt tataaatgtt tctaatatct ttgttgtctc
     5341 actggggata attaagaccg actcggtctc tgccggaacc aggacggtct ctccctgccc
     5401 gataaaaaca gaatttccat tgttgtcttt caacgtacag tgtccctgca tacaaatata
     5461 gattacaaaa gagtcgagag cgttatagtg ccgtgccaac gggatatcca tttccagcag
     5521 attcgttgag aagtactcac acgttaccag ttctaccggt tggttaagac gatttgtata
     5581 ctcggtctga taattaccat acacgttgta atcgatagca tcttttgcta attctgtatg
     5641 taattcgcgt aaatttccct gtgcatcctt tctgttgtaa tcgtagatac gataagtgag
     5701 gtcagatgtt tgctgaattt ctgcaatgaa gcatcctgca ccaatagcgt gaaccctgcc
     5761 cgcaggcagg aaaaatacat cgcctgtttt tacgtcgtat ttttgaagtt tatcggtaaa
     5821 gctgttgttt tctattgtct tgatatactc atcggaggtt atctgtgttg aaaaccctga
     5881 ataaagaaaa gcatcggagg aggcttttat tacgtaccac atttctgttt ttccgaagga
     5941 gttgtgtcgt tcttttgcta gcttatcgtc cgggtgaact tgaatggaca gatcatcacg
     6001 ggcatcgata aacttgataa gaagtgggaa ggtattgcca aacttctcat atactttttt
     6061 ccctactaat cgggctttgc tggtccgaag gacttcatcg atcggttttc ctgccaactc
     6121 tccattgcca acgatggata tcttgccttc cacccccgat atttcccaac tttctccgat
     6181 accgtcttct gcaggtgtaa tgtttttaaa tttacaaata tcggaaccac cccaaatcat
     6241 cggtctgagg atgggttcaa actttaaagg atataacgcg acccctgaat ccccttcaga
     6301 ggattgggcg gcatgctgct tattagcgta agtattcatt gttttttaat ttgttaaaac
     6361 aggtcttctt gtttctcccg gattttcggg attgtataca gaaaaaatga actgttcaag
     6421 taaaatttgt tttccggctc ccttaaaggg ccggagtcat atttttttta aaacaaaggc
     6481 agaacgggcc ggcaggtata ttttaatcca gccttttccg tcttttgcat acaacggatc
     6541 gtgatgcgtg aaatgatgaa cggtatcgtt gttcagaccg aaaccgccga acctgaagtt
     6601 gtcggtgttg agaacgatgc tgtactctcc ttcaggaacc aacatgccat aatcattaaa
     6661 cgatttttcc ggatggaagt taaagacaaa caacagcctt tcccgcatat aagccagcac
     6721 gttgtcgtcg tctttgtccc agattttcgt gatagttgtg gtctgaaagt cgggaacgga
     6781 ttttatgaga ttaatcattg ctttatcaaa gtcccccaag taatggtact tgaggttttt
     6841 gtcgtcagcc aactgccatt ggcgccgggc gtatttgtga gaccagttgt tcccttcccg
     6901 cggaaaatca atccattccg gatggccgaa ctcgttgccc ataaaattca ggtagcctcc
     6961 gtttatggtg gtggccgtaa ccagccggat catcttatgt aatgccattc ctctgtctac
     7021 ccggaacgaa ctgctcttca gtttcgacat gaaccaatac atgtcggcgt ctattagcct
     7081 gaaaataatg gtcttgtctc ccactaatgc ttggtcatgg ctttccacat aagagattgt
     7141 tttctcgtct gcacgacgat tggtagtttc ccaccaaata gcggaggggt gccactcttc
     7201 gtcttttcgc tccttaatgg tcttgatcca gaaatcgggg atattcatcg ccatccggta
     7261 atcaaaaccg tatcctcctg attttatgtc caaccccaag ccaggcatgc cgctcacttc
     7321 ctctgcaata gtgatagcag tgggatttac ttcgtgaatg agcttgttgg ccagtgtcag
     7381 ataacaaatg gcatccaagt cttgtctgtt gttgtaataa tcatcatacc gggtgaagct
     7441 ctcttccagg ccgtgactgt agtacagcat ggaggttaca ccatcgaaac ggagaccgtc
     7501 gaatctatat tcctccatcc agtatttgca gttcgaaagc agaaaatgga ggacttcatt
     7561 tttgccgtaa tcaaagcaaa gtgaatccca ggctggatgg tttctgcggt tgggattgct
     7621 tgggaaatac aggtcatacg agccatcaaa acgggcaatc ccttccaatt cgttctttac
     7681 tacatgtgaa tgcacaatgt ccataatcac cgcgattccc atttcatgag ctgcatctat
     7741 aagttggcga agttcatcgg gtgtgccgaa gcgcgacgtt ggcgcaaaaa aattggatac
     7801 atggtaacca aatgagccgt aatagggatg ttcctgaata gccattaatt gaatggcatt
     7861 gtatccgctg ttcttgatcc ggggcaacac gttttcccta aactcgttat aggttcccac
     7921 cttttcttct gcggtagcca ttccaacgtg acattcgtat ataaggagtg gcgacgtgtc
     7981 tggtttaaaa ttagttattt tgaaggtata tggagttgcc ggctcccata cctgtgcact
     8041 gaatatatgt gtctgggcgt cttgcacaac cctacggcac caggcgggaa tccgttcccc
     8101 agatccaccc tcccagctga ctaaaagttt gtataagtct tggtgacgaa tgtgttccgg
     8161 tggaattttt acttcccaaa cgcctttttc aatacttctc agccggtacg gttccattcg
     8221 ttgccagtta ttgaaggtgc cgataagaaa aatttcagta gcgtttggtg cccactcacg
     8281 aaaaacccat ccatctgttg tcttgtgtag tccaaagtat agatatcctg ttgcgaagtc
     8341 cgacaacctg atctttccgt tttgagtcaa ctccttttct tttttcaaga agtaattgtt
     8401 ccggtcttct atgcttcgac tgaaaggttc aagccaaggg tcatttttta cgatatccaa
     8461 catgaattac tcagtaatta tttatagtta taaaacagca actttcagga tgatcttccg
     8521 ggtaatgcct tcgccgataa gtggggcgtg tgcatcttcg ggcgagaata taataaattc
     8581 gcccggaagc acgcggatat aggtggacgg ttcgtcttca aacaactgga aatcattttg
     8641 gctgtcgtat tcctgcgtcg gcatctttag gtttttcaag gatttccatc cgaaaatttc
     8701 ttcccgtgtg ataggaatct gaatatcgat gaattttctg tgagtttcca gtttagtttc
     8761 tgttttgctt ttcaaggcga tttttactgc cgacgctttc aggtatacgc cttctatttc
     8821 ggaagttcct tctggcaaat tgtccacatc taatcctctg acatactcga aagccttttc
     8881 aaacaaagga ttcaatccaa aataattggg tgcgttttct atgttgtcca atatcataat
     8941 tgtccgtttt tttatcttat ttgcacaaag ataacggatt ttatgaacat ttagtttcat
     9001 ttattctcaa aataagcgac tcgcggattt caatcagaaa aaaaacgtat ctttgttgtt
     9061 tgataaacga tactgctatg aaacgaatat tacccgcatt cattatacta ctaatatccg
     9121 ttgccgtttt ttctcaaaat gatcagacca cctgcaacct gggattctcg tttaaaataa
     9181 gcaataattc caactggggt aataacgaac ctgttgttac ggaggttgtt cccggatctc
     9241 cagccgaaaa agcaggttta aaagccaacg atattattct tgaagtgaac ggaaacggga
     9301 cttacctgaa gccgtcacat accataatgt cgtggtttat ggagaaacct tcagaaatgt
     9361 cgatatccat acgcaacttc gaagcgtcgt tcaagccgat gcatattgct aaagattgca
     9421 ggccgagaaa cggattgagt gaagcacaac tggctcctgt gttttcgttt tatagccttg
     9481 aggatattca ggaccggaaa tttattattc cggtaaagac gaccataaac cccgatgctg
     9541 atttttttaa ttaccggacc tatgattttg ctccttctga tgtaagttca cgagaaatgg
     9601 acgaacgcat taattcaata tttgtgaggg tgctgtctca gctcggtctc aagcgcgaca
     9661 gtgaagaccc cgattttatc attcagacct tttacagtta ccagaacaat cccatgttca
     9721 aaacggaatc gcctacgcgt ggaacctatt ccggaacctg gcgattcgat acccgtaaca
     9781 accggatggt taagattcct gtatttgatc ctactcaacc tgttagaatt gatgatgtga
     9841 tgtatgacct ggaatttggc taccggtttt atgaccggaa gtttacggaa cccggcaggt
     9901 cgatgctggt ctgggagagc gaggttaaag aaaagctgag tgataattac gggttactgg
     9961 actacctgga aatgaatctc cctttgatat tgagtaaatt tcctaattca ggaaacctgg
    10021 aaagagccac ttaccacgtg aagtatttgc ggtataacta tacaggtatt agttacgatt
    10081 tgaatgattt aaaaacagtg gtttctgttg atgccgggtc tccagcagcc cgcgcaggga
    10141 ttaaaccggg ggatgttgtg ataaaagtgc aaggccacaa tttcaaccac gatgcagcat
    10201 cgcttacttc cagttaccgg cggtttattg ctgaaacaat gaaatatcgc gatccagcga
    10261 caaaatatac cgattcaaac gggtttcaaa atgccatgta ctgggatata atacattata
    10321 acagcatttc taaggagatt aatgacaaaa aaaggtataa ggccggattc tcatacctgt
    10381 tcaatttcaa tcaatatatt gactgggaca ctccagatac actgaatatt gacgttgaaa
    10441 ggaaaggaga gaaattgtcc tttgaggtga aacctatcat aaacagacac tcccatgttt
    10501 ccgtggaata aatttcctct ccggagacct gtttgattat agaaactttt ctgaattttt
    10561 attttttcaa catagcccgc tattatatgt gagctgaaaa gccaggtcca tcaaaaaaat
    10621 agacttaaaa taggacatga aaaaaatcaa aacggtttct tgataaaaaa aataaaaaaa
    10681 agcggtaaaa aatttgccga taaaattaaa tgctctatct ttgtatccct ttcgcaataa
    10741 tcgaaagggt ttgttattga gataacaaat aaagttgcaa aagttgaaaa attgaagttg
    10801 tagaatcgac tggaaatcaa gttgaaaaaa tttttttcaa aaaaaattca gattttgttt
    10861 tgtcaattca aaaatagttt ctacctttgc atccgctttc cgaaaacaag tagatgtaga
    10921 aagcaattga tcgtt
//
LOCUS       DAOSDR010000045        10823 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrimonas sp. isolate AMR_MDS_1826
            NODE_2332_length_10823_cov_4.061200, whole genome shotgun sequence.
ACCESSION   DAOSDR010000045 DAOSDR010000000
VERSION     DAOSDR010000045.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863355
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrimonas sp. (wastewater metagenome)
  ORGANISM  Petrimonas sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Dysgonomonadaceae; Petrimonas.
REFERENCE   1  (bases 1 to 10823)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10823)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:10:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,720
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 35
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10823
                     /organism="Petrimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2332_length_10823_cov_4.061200"
                     /isolate="AMR_MDS_1826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2023866"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..725)
                     /locus_tag="PLI30_02985"
     CDS             complement(<1..725)
                     /locus_tag="PLI30_02985"
                     /inference="COORDINATES: protein motif:HMM:NF021778.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-propeller fold lactonase family protein"
                     /protein_id="HOI78551.1"
                     /translation="MKRKIIFALIFAVLFAACTGRKTKKEDMDTMNKVVASATPDDMF
                     LLVGTYTSEGGSQGIYVYKFDTETGKSDSLSLAEAVNPSYLVVSPDEKFVYAVGENGD
                     GESTAVTFAFDKGRGVLQYLNSSNTESPGPCFIEIDKTGKSVLTANYGGGSISVFQVN
                     DDGTLSSADPVIGFEGSGPDTTRQESSHLHSVRFSPDGKFLFAADLGTDKIYRYNAIG
                     SVFEGQPVLSQASLKEFSTPAGTG"
     gene            986..1426
                     /locus_tag="PLI30_02990"
     CDS             986..1426
                     /locus_tag="PLI30_02990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005854873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5606 domain-containing protein"
                     /protein_id="HOI78552.1"
                     /translation="MLTKILSVSGKPGLYKLISTSKNLNIVESLLDGKRIPVYLTEKV
                     VALSDVSIYTTEEDMPLREVFQKIKEKENGEKTVLGSKSSNGEVFRYFEEILPNYDTE
                     KVYASDIKKILNWYNILINSGIDFDQEEPNTEEPAGTEPENNEK"
     gene            1433..2269
                     /locus_tag="PLI30_02995"
     CDS             1433..2269
                     /locus_tag="PLI30_02995"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: protein motif:HMM:NF013327.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HOI78553.1"
                     /translation="MNPKNLFTRIVAGIIYIAVILVGILGGKYSFIGVFGLFLIIGLY
                     EFYRMTEKNTSHAISKAFNIASGFLIFLSAYLYLENICKIALPLTSITYLLILFASAI
                     FINRKDILHAIIYSFFGQIYITLPLSILMLLSYQYQTLSNEYYYAFVLAIFVFIWVND
                     TAAFVVGSLLGKHKFIERISPRKTVEGFIGGIVFSVSAAVILSAFFTDYSLAFWIGFS
                     IITALFGTLGDLFESLIKRTYEVKDSGSLIPGHGGILDRIDSLLIAIPAVYIYLAVML
                     IS"
     gene            2366..2584
                     /locus_tag="PLI30_03000"
     CDS             2366..2584
                     /locus_tag="PLI30_03000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019539204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HOI78554.1"
                     /translation="MNKKAIGTNAGIIWNLLNSSEKWDVSQLMQKSGLTEIEVYTAIG
                     WLAREDKIEIEEKPNNKHYYYLIVDYYF"
     gene            2728..3498
                     /locus_tag="PLI30_03005"
     CDS             2728..3498
                     /locus_tag="PLI30_03005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI78555.1"
                     /translation="MKKLLSLFLLISLILISCEKIDSSLIGSWRLNETNYLITANQDF
                     TFTNVFGSPWKGTIKIDNTNLNPMLFNYYFENVWGQTLFASENVSFTFAGPNRLEIYY
                     ENALYLLEKEYIFNISTGLFKVDGTAKYQDRTLQIAIELTMPKVELKKGAQFLVKDGY
                     NFMPYRHLWLNNGGKLQTDYLTGDIMDRLSGKWNISDNKININLKNRASDIYQYRFNN
                     DKLLLSKEKNTKEQLPHHISPFADKISNTTYQALYTRE"
     gene            complement(3574..4608)
                     /gene="galE"
                     /locus_tag="PLI30_03010"
     CDS             complement(3574..4608)
                     /gene="galE"
                     /locus_tag="PLI30_03010"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006800683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HOI78556.1"
                     /translation="MSKKILVTGGTGYIGSHTVVELQQAGYEVIIIDNLSNSNAEVIE
                     GIFKITGVRPVFEKLDCIDFQALKSLFEKYQGIDGIIHFAASKAVGESVQKPLMYYRN
                     NLVSLINLLELMPQFHVKGIVFSSSCTVYGEPDNNPIDESAPIKPAESPYGNTKQINE
                     EIIRDFIYSGAPVKSIILRYFNPIGAHPSAEIGELPIGVPQNLVPYITQTGIGIREQL
                     SVFGNDYNTPDGSCIRDFINVVDLAKAHVTAVERMLSDKSDEKVEVFNLGTGKGVSVL
                     ELIDIFEKVSGKSLNYNIVGRREGDIEQIWANPDKANNVLGWTAKETIENTMASAWKW
                     QQRLRERGIM"
     gene            4821..5804
                     /locus_tag="PLI30_03015"
     CDS             4821..5804
                     /locus_tag="PLI30_03015"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOI78557.1"
                     /translation="MEYQVKIYGKKEDLPPLNDVKFFHYVSSFDWNQNISFYRPMMLV
                     VFCDEKPIASMFALIMRINRLMYGSVFKRCYISQQPSFYEENVNKIEIFNLLISSLVK
                     EVENKVFFIEYRNLNDAVFGYKGFRENGFYSAKWINVRNSLQRKRKIWDQLSSTRKNQ
                     VNKARRKGVVVEELTSHDKLREIYKLIDKSRNWKMSKKFPPYQYFENFFNCYVSLGKG
                     KILISRYNEKIIGGIILGFEQKTVYVLYFWGKEKRYKTYYPTVFTIFSAMEMAEKEGY
                     EFFDFMDSGYLNINAGKPRFLLQFGGKPKSTRRWYRFNWRLLNFFAKKIHD"
     gene            complement(6069..6812)
                     /locus_tag="PLI30_03020"
     CDS             complement(6069..6812)
                     /locus_tag="PLI30_03020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006800739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HOI78558.1"
                     /translation="MKLIKRIFIFLYQWLIFAPIFLLFTILTALTVMVFAPVFGSRYW
                     GYVPPRWWSRMTCWLALCRIKSSGHENLNSKQSYVFVANHQGAFDIFLIYGFLNQNIK
                     WVQKASLRNIPLVGFASEMAGHVFVDNSSPAARANTIREAKKKMTDDVSIMLFPEGAR
                     SRTGKLGHFKRGAYQIAYDLRLPIVPLTINGPFDVMKRGSLCLKPNKLELIIHKPICT
                     ENLNECDIPTLIDESRKIIHSALWEKFKD"
     gene            7002..7286
                     /locus_tag="PLI30_03025"
     CDS             7002..7286
                     /locus_tag="PLI30_03025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI78559.1"
                     /translation="MKELARYSGVIVMIIGVLILALPFFSGTTNNTNLLIGLLLVIEG
                     FLGHLYVNNMKKGTIVGNIVWAIILLVVPYFLFTAMKNLAYSKDEFALYN"
     gene            complement(7342..8424)
                     /locus_tag="PLI30_03030"
     CDS             complement(7342..8424)
                     /locus_tag="PLI30_03030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019539217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III"
                     /protein_id="HOI78560.1"
                     /translation="MKKIYSLITGTGSYVPSKVVKNDDFLNYDFLESTGEKIDKENRE
                     IVDKFEEITTISERRYAEDHQLTSDLALIAAEQAIESAGIDREELDYVILAHNFGETQ
                     PNDTRIDILPTLASRVKAKLGIRSPQAIAYDILYGCPGWVQALIQADYYIRSGDAKKI
                     LVIGADVLSRISDPHDRDSMIYADGAGAAIVEAVESETPVGIIGHNSRSDSMDYVNLL
                     HMGYSYDRELADKKDLYLKMNGRRLYQYALENVPKAIKSGLDKLKLHVNDVKKVLIHQ
                     ANGKMDDAILKRLFKLYNINDIPESVMPMTISWLGNSSVATVPTLLDMVMRGKMNGHS
                     ISSGDNLVLTSVGAGMNINSVIYRMP"
     gene            complement(8631..9644)
                     /locus_tag="PLI30_03035"
     CDS             complement(8631..9644)
                     /locus_tag="PLI30_03035"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HOI78561.1"
                     /translation="MNKLKLSILDLAKFTEETKTASEVLNQSTEIAKLADKLGYTRYW
                     FAEHHNTSMVMSMFPEIMVAHVASQTQRIRVGTGGVMLPNHSAFSVAERFAMLEALYP
                     GRIDLGIGRAPGTDGRTALALRRSWEAIKQDTFPEQLTELLGFLGHNLPDKHPFAHIN
                     ASPDPFLIPEIYMLGSSTGGVEFALTKGLPFVFAAQINADLAVPVLTMYRRQFKPSEY
                     LQEPKSILSIMVFTAETDEEAYYQAAPAILHWTMLTVGKRIVNPTFEEALSYRYSLQE
                     EAVKQSIMRKFVIGSPGKVSEMLWQWAKETLVDEIIIFDLYSQMKGRRQGYELLAKEL
                     GLS"
     gene            complement(9655..10368)
                     /locus_tag="PLI30_03040"
     CDS             complement(9655..10368)
                     /locus_tag="PLI30_03040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019540453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3307 domain-containing protein"
                     /protein_id="HOI78562.1"
                     /translation="MIVLVKLILMHLAGDFILQPKSWVEEKEKQGAKSLKLYLHGLIH
                     GALVWLVLWDLRHWAVALSITIVHVGIDMVKLFFQKKNNKTGWFLMDQLLHVLSIVVL
                     WYLFFNPDISFGALAENLQFWIYVTAILFLTVVCGIGIQVLLTNWTKDIYLDKEKSLP
                     NAGRYIGILERLLVFLFVVLGRWEAIGFLIAAKSVFRFGDLKDSRNRKLTEYILIGTL
                     LSFGIAIVTGILTLYISTV"
     gene            complement(10365..>10823)
                     /locus_tag="PLI30_03045"
     CDS             complement(10365..>10823)
                     /locus_tag="PLI30_03045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI78563.1"
                     /translation="ALRVKALIKKIVSDETNKSLIDVRMAIGIGGKSYSGDRITESNG
                     EAFINAGEKFEELEKEKFNLLVKSPWPDFDGEMNLFIRLASVFMDKWSVASAELVEVA
                     LEHPNYTQNEIGRRLGIKQNSVSGRWKRANIDEVLSMEQMYRRKLEMLLL"
BASE COUNT         3292 a         2290 c         1972 g         3269 t
ORIGIN      
        1 ccagtaccgg cgggagtaga gaattctttc agacttgcct gggaaagtac cggctggcct
       61 tcaaaaaccg agccaatagc gttatagcga tatattttat ctgtccccag atcagcagca
      121 aacaggaatt ttccgtcagg agaaaatcgg acgctgtgaa ggtgagaact ctcctgtcgg
      181 gtagtatcgg gtcctgatcc ctcaaaacca atcacggggt cggcggaaga taatgtgccg
      241 tcatcattta cctgaaatac agaaatgctt ccacctccat aatttgccgt gagaacactt
      301 ttgcctgttt tatcaatttc aataaagcac ggtcccggac tttcggtgtt gctcgaattc
      361 aaatactgaa gcactcctct gcctttatca aacgcaaatg taacggcagt actctctcca
      421 tcgccgtttt cacctacagc gtacacaaat ttctcgtcag gacttaccac cagatacgat
      481 ggattcacgg cttcggccaa gctcagagag tcggatttgc cggtttccgt atcaaattta
      541 tacacataaa taccctgact gcctccttca gaagtatagg ttcccacaag taaaaacata
      601 tcgtctggcg ttgccgatgc gacaacctta ttcattgtat ccatatcttc tttctttgtt
      661 tttctgccgg tgcacgctgc gaataaaaca gcgaagataa gtgcgaaaat aatttttctc
      721 ttcatttgta ttttatttgt ttgttagctt gaagtcaagt tattgttata atattcactg
      781 aatttgcaca cgtatggtga tccttgctga atctttcaac gaacgatact ttgtatctag
      841 tttaatacga attaatctga cacatgcgaa attacatctt tttacggata taaccatctc
      901 cgagaaaaac aattgccgtt ttatgttgtg aaattgccgg agactgtgta tttttgcaaa
      961 ctattttgaa ctaaaaaaaa caaatatgct aacgaaaata ttgtctgttt ccggaaagcc
     1021 gggtttatat aaactaatct ctaccagtaa gaaccttaat atcgtggaat ctcttctcga
     1081 tggcaaaaga attcccgttt atttgaccga aaaagtagtt gccctcagcg atgtatctat
     1141 ctacacaacc gaggaagata tgccgttgcg cgaagttttt cagaaaataa aagaaaaaga
     1201 aaatggagaa aaaacggttc ttgggtcaaa gtcatccaac ggagaagttt tccgctattt
     1261 cgaggaaata ctgcctaact acgataccga aaaagtatac gcttcggaca taaaaaagat
     1321 attgaactgg tataatattc ttatcaacag tgggatcgat tttgaccaag aggagcccaa
     1381 taccgaagaa cctgccggaa ccgaaccgga aaacaacgaa aaataaagta aattgaaccc
     1441 taaaaatttg ttcactcgga tagtagcggg aattatatac attgccgtta ttttggtggg
     1501 tattctggga ggtaaatact cttttatcgg agttttcggt ttatttttaa ttatcggact
     1561 ttacgaattt taccggatga ccgaaaaaaa cacctctcac gccatcagta aagccttcaa
     1621 tattgcttcc gggtttctta tttttctttc ggcatatctt tatttggaga atatttgcaa
     1681 aattgcattg cccttgacct ccataaccta cctgcttatt ctttttgctt cggcaatctt
     1741 cataaacagg aaagatattc ttcatgctat catctattcc ttttttggac aaatctacat
     1801 tacgctacca ctatccatac tgatgctttt gtcctatcag tatcagacac tttccaacga
     1861 gtattattat gcttttgttt tggctatttt tgtttttatc tgggtaaatg atacggcggc
     1921 ttttgtggta ggttctctcc tgggcaagca caagtttatc gaaaggattt ctcccaggaa
     1981 aaccgtcgaa ggtttcatcg ggggaattgt attttcggtc tcggccgcag ttattctttc
     2041 agccttcttt accgactatt cgttagcttt ctggatagga ttcagcatta ttacagcact
     2101 cttcggcact ctgggggacc tgtttgagtc actaattaaa agaacttacg aggtaaagga
     2161 ttctggtagc ctcatccccg gacacggagg tattctcgac cgaatagata gcctgcttat
     2221 tgccattccg gcagtctaca tttatctcgc tgttatgctt atttcctaac cttgtttttt
     2281 ctaaaaaaaa caggttgttt tcattgaact tttgtagcgt attgctgttt caattaaaat
     2341 tgtcatacac ttaacacgta ttaagatgaa caagaaagca attggaacaa acgccggcat
     2401 tatctggaat ttgttgaaca gcagtgagaa atgggacgtc tctcaattga tgcaaaaatc
     2461 aggcttaacg gaaatagaag tgtacacggc tatcggttgg ctagcccgcg aagataagat
     2521 agaaattgag gaaaaaccaa acaataaaca ctattactac ctcattgtag actattattt
     2581 ctgatcaaat ctgagaacga aaataaaaat ccgacatcag tgacgatgcc ggatttttat
     2641 tttttaacac ttatgaatgc aacgttttcg gccgtttctc catctatata ttgttaacaa
     2701 ctcaattagg tataaaatcg gcccaaaatg aaaaaattac tgtctttgtt tttacttatt
     2761 tcgctcatct taatttcgtg tgaaaagatt gattcatccc ttatcgggag ctggagatta
     2821 aacgaaacaa actatcttat aaccgccaat caggacttta cgtttacgaa tgttttcggt
     2881 tctccttgga aaggaacaat aaagatcgac aacacaaacc taaatccaat gttattcaac
     2941 tattatttcg aaaatgtctg gggacaaaca ttgtttgctt cagaaaatgt aagttttacc
     3001 tttgcaggtc ccaatcgttt agagatttat tatgaaaacg cactttattt acttgaaaaa
     3061 gagtatatat ttaatatttc aacaggatta ttcaaagtgg atggaacagc caaataccaa
     3121 gacagaaccc tacaaatagc cattgaatta actatgccga aagtagaact gaaaaaagga
     3181 gctcaatttt tagttaaaga cggatacaat ttcatgccat atagacacct ctggttaaac
     3241 aatggaggaa agttacaaac tgactattta acaggcgaca ttatggacag attatcagga
     3301 aaatggaata taagtgacaa taaaataaac attaatttga aaaatcgtgc aagcgatatt
     3361 tatcaatacc gatttaacaa tgataaattg ctgttgtcga aagaaaagaa tacaaaagag
     3421 cagcttccgc accacatatc gccgtttgcc gataaaataa gtaataccac gtatcaagca
     3481 ctttatacgc gggagtaaca aaaaatccga aacaggaata tctcggattt tatttaaatt
     3541 tgtaaatcta aaattgtaat tcgtaaatta aaatcacatt attccccttt cgcgtaaacg
     3601 ctgctgccac ttccaagcag aagccattgt attttcgata gtttcttttg ctgtccagcc
     3661 aagaacgttg ttcgctttat cgggattagc ccatatttgc tcaatatcgc cctctcttcg
     3721 ccctacaata ttataattca gcgacttgcc agaaactttc tcgaaaatat caatcagttc
     3781 caacaccgac accccctttc ccgtgccaag attaaaaact tccacctttt cgtccgattt
     3841 atccgagagc attcgctcaa ctgcggtcac gtgcgctttg gccaggtcca ccacattgat
     3901 gaaatcgcga atgcatgaac cgtcaggagt attgtaatca ttaccgaaaa cacttagttg
     3961 ttcacggatt ccaatgccgg tttgcgtaat atacggcaca agattttgcg gtaccccgat
     4021 aggcagttct ccgatttcgg cggatggatg tgctccaatt ggattaaaat aacgcaaaat
     4081 aatgctcttt acaggtgcac ctgaataaat aaaatcccga attatttctt cgtttatttg
     4141 tttagtattt ccgtaaggcg actcagcggg ctttatagga gcactttcat cgataggatt
     4201 gttatcgggt tcaccgtaaa ccgtgcatga ggatgaaaaa acaatccctt tcacgtgaaa
     4261 ctgaggcatc agttccaaca ggtttatcaa cgacaccagg ttattgcggt aatacatcag
     4321 cggtttttga accgattcac ccaccgcttt actggcagca aaatggataa taccatcaat
     4381 accctgatat ttctcaaaca aggatttcaa tgcctgaaaa tcaatacagt cgagcttttc
     4441 gaaaacagga cgtactcctg taattttgaa aatcccctca attacctctg catttgaatt
     4501 ggataaatta tctataatga tcacttcata accggcctgt tgcagttcaa ctaccgtgtg
     4561 cgaaccaata tatccggttc cacctgtaac aagaattttt tttgacataa agaataattt
     4621 cagcttaaag taagccacaa agttaataaa ataacaggtg atttatctgc ttttctgtta
     4681 tttcccgtta aaaactaagt ctacggcata cgattccctt atccggtaaa atgccgtaaa
     4741 ggccaattaa aaaaaccaat attgccgtta aaatatgttt taatcaaagt ttttttctat
     4801 ttttgtgtag attgcaaaca atggaatatc aggttaaaat atacggtaaa aaggaggatc
     4861 ttcctccctt gaacgatgtg aaattctttc actacgtatc ttcttttgac tggaaccaaa
     4921 acatttcatt ttacaggcct atgatgcttg ttgttttttg cgatgagaaa cctatagcat
     4981 ctatgtttgc actaattatg cgaatcaatc ggctgatgta cggctcggtg tttaaaagat
     5041 gttacatttc gcagcaaccc agcttttacg aggaaaacgt caacaaaata gagattttca
     5101 acctactcat ttcttccttg gtgaaagaag ttgaaaacaa ggttttcttt attgaatacc
     5161 gaaatttaaa cgacgcagtt tttgggtata agggatttcg tgaaaatggg ttttattccg
     5221 caaaatggat taacgtgaga aactcgttgc agcgaaaacg aaagatatgg gatcagctgt
     5281 cttccacccg gaaaaatcag gtcaacaaag cccggcggaa aggtgttgtt gttgaagaac
     5341 tcacctcaca cgataaatta cgggagattt acaaattaat cgacaaatcc agaaactgga
     5401 aaatgtctaa gaagtttccg ccttaccaat acttcgagaa ttttttcaac tgttacgttt
     5461 ctctgggaaa gggaaagata ctgatcagcc gttacaacga gaaaatcatt ggtggaatta
     5521 ttctcggatt tgagcaaaaa acggtttatg tactttattt ttgggggaaa gaaaaaaggt
     5581 acaaaacata ttatcctacc gtcttcacaa ttttttcagc catggaaatg gctgaaaaag
     5641 agggatatga gtttttcgac tttatggact caggctacct gaatattaat gcaggaaaac
     5701 ctcgtttcct gcttcaattc gggggaaaac ccaaatcgac aagacgctgg taccgcttca
     5761 attggcgctt actcaatttt tttgcaaaaa aaatacacga ttgacgggga taaaaaaact
     5821 ttccggaaac ctgtaatgca aaattgtgta aacaattttg tatgcactcg attgcataaa
     5881 gccttttaga ttaaataaag ttaagctagg ccattttttt attcattcat ttggctgtaa
     5941 ataaaaagca cgctatattt gtatcgtgtt tttcatggta ttagatttaa ggttaacaat
     6001 ggagattggt tgtcgtgaga caaccatttt ctttttatat tgatccacac tccggattcc
     6061 ttttagtttt aatctttaaa cttctcccac aaagcagaat gaatgatttt ccttgattca
     6121 tctatcaatg tagggatatc gcattcattc aaattttcag tacaaatagg cttgtgaata
     6181 atcaattcca gtttattggg ttttaaacaa agtgagccac gtttcatcac atcgaaaggg
     6241 ccgttaattg ttagcggaac gataggtaat ctgaggtcgt aagcaatctg ataagctccc
     6301 cttttaaaat gtcccagttt cccggtacgt gaccgggctc cttcaggaaa gagcataatg
     6361 gaaacatcat ccgtcatttt ctttttggct tcacgaatcg tattggcacg agctgcaggg
     6421 ctggaattat ccacaaaaac gtggccggcc atttccgatg caaagcctac caaaggtata
     6481 ttgcgtaagc tggctttttg tacccatttt atgttctggt tcaaaaaacc gtaaattaaa
     6541 aaaatgtcga aagctccttg atgattggct acaaaaacat aagactgttt agaattgaga
     6601 ttttcgtgtc cgctgctctt tatccggcat aaagccagcc agcaagtcat tcttgaccac
     6661 catctcgggg gcacatatcc ccaataccga cttccaaaaa cgggagcaaa gaccattacc
     6721 gtcaatgctg ttaaaatagt aaacagaagg aaaatgggcg caaaaataag ccactggtat
     6781 aaaaatatga aaatcctttt tatcaacttc ataacacaag aaaattcgca ctaacaaaaa
     6841 taatcatttt ttgctatccc ctgattataa aaaaggttgt tttcaacttc atgaaggttt
     6901 ttgatgtcat aattgaattt ttagcttaaa taattttatt atttgtggaa ttttgtgttt
     6961 atttgtgcta cgaaatcaaa aacataatat aataaacaca catgaaagaa ttagcaaggt
     7021 attcaggcgt aatagtaatg attattggcg tgctgatttt ggcgcttcct ttttttagcg
     7081 gaacgaccaa caacacaaac cttttaattg gtttactatt ggtaattgag ggttttttgg
     7141 ggcatctcta tgtgaataac atgaaaaaag gaactattgt gggtaacatt gtctgggcaa
     7201 ttattcttct ggttgttccc tattttttgt tcacagcaat gaaaaacctg gcatacagta
     7261 aagacgagtt tgcgctgtat aattaattac aaaaaatgca taacacaaaa aaagaggatt
     7321 aaaaaatcct ctttttttgt gttatggcat cctgtatatg acgctgttaa tattcattcc
     7381 tgcacctacc gatgtaagca ccaggttatc gccggaggag atgctgtgtc cgttcatttt
     7441 tcctctcatc accatatcga gcaaagtggg aactgtagca acagatgagt ttcccaacca
     7501 cgaaatcgtc ataggcatta ccgattctgg aatatcgttg atgttgtaca acttaaacag
     7561 tcttttgaga atggcatcat ccatttttcc gtttgcctga tggatcagta cttttttcac
     7621 atcgtttaca tgcaatttaa gcttgtccag acctgatttt atagctttgg gaacattttc
     7681 caatgcatac tgatataaac gacggccgtt cattttcagg tataaatcct ttttatcggc
     7741 caactctctg tcgtacgaat atcccatgtg aagcaagttt acataatcca tggaatcgct
     7801 tcgcgaatta tgcccgataa tacccaccgg cgtttcgctt tccactgctt ctacaatggc
     7861 agcaccagct ccatcagcat aaatcatact atcgcggtca tgcggatctg aaatcctcga
     7921 tagtacatcg gcaccgatga ccagtatctt ttttgcatca cccgaacgaa tataataatc
     7981 tgcctgaata agggcttgaa cccagcccgg acatccatac aaaatatcgt atgcaatagc
     8041 ctgtggactc cggataccaa gtttagcctt tacgcgcgaa gcaagcgtag gcaatatatc
     8101 aatacgggta tcattcggtt gggtttctcc aaaattatgt gccagaatca cataatcaag
     8161 ctcttccctg tcaatccctg cagattcgat ggcttgttct gctgcaatga gagccagatc
     8221 agaggttagt tgatgatctt cggcatacct tcgttcggaa atagttgtta tttcttcaaa
     8281 tttatccact atttcgcgat tttctttgtc gattttttca cccgtcgact ccaggaagtc
     8341 ataattcaaa aagtcatcat tcttaacaac tttggaagga acataacttc cggttccggt
     8401 aatcaggctg tatatttttt tcatataata aagtaataat atttattttc agatgaaggt
     8461 cgtataatcg tttcagcgca actcaaaccg ctggcttcac gctcgttcca tcatcaatct
     8521 ccacaaacaa taacctaatt tctcagcaaa ggtagcaatt tattatcaat acaattaaac
     8581 ttcaccggtt tttcagcagg gcatatccct tttttctaca atacctgaaa ttaacttaat
     8641 cccaattctt ttgccagcaa ttcgtaaccc tgtcggcgtc ctttcatctg cgaatacaaa
     8701 tcgaaaataa tgatctcgtc caccagggtt tctttggccc attgccaaag catttcggaa
     8761 actttgccgg gtgagccgat aacgaacttc cgcataatgc tttgcttcac cgcctcttcc
     8821 tgaagtgaat acctatatga aagcgcttct tcaaaagtag gattaacaat cctcttacca
     8881 acagtcaaca tcgtccagtg tagaatagcg ggcgctgcct gataataagc ttcttcatcg
     8941 gtttctgctg taaaaaccat aatcgaaaga atacttttag gttcctgaag atattccgaa
     9001 ggcttaaact gccggcgata catggtaagc acagggaccg ccaaatctgc gtttatctgt
     9061 gccgcaaata caaaaggcaa tccttttgtc aaggcaaact caacaccacc ggtactggaa
     9121 ccaagcatat agatttcggg gatgagaaat gggtctggcg atgcgttgat gtgggcaaaa
     9181 ggatgtttgt cgggcaggtt gtggcccaag aatcccagaa gctccgttaa ttgttcggga
     9241 aacgtatcct gcttaatggc ctcccacgac cttcgaagag ccagcgctgt ccgtccgtcg
     9301 gttcctggtg cacgtcctat acccagatct attctgccgg gatacaatgc ctcaagcata
     9361 gcaaaccgtt cggcaacact gaaagcactg tgattgggca acatcactcc tcccgttcct
     9421 acccggatcc tttgggtttg tgaagcaaca tgcgccacca taatttcggg gaacatgctc
     9481 atcaccatag atgtattgtg atgttcagcg aaccagtaac gggtgtatcc cagtttatcg
     9541 gcaagtttgg caatttccgt ggattgattc aatacttctg aagcagtttt ggtttcttcg
     9601 gtaaattttg ccaaatcaag tatggacaat ttcaatttgt tcattccggt tttgtcagac
     9661 agtagagata tataaagtga gaattcccgt aacaatagct attccaaaac tcaacaaggt
     9721 cccaataaga atatactctg tcagtttccg gtttcgcgaa tctttcaaat cgccaaaacg
     9781 aaatactgat ttagccgcga tcagaaaccc aatagcttcc caacgcccca aaacgacaaa
     9841 caggaaaacc agaagtcttt ccagaatacc gatataacgg ccggcattgg gtaacgactt
     9901 ttctttatcg aggtatatat cttttgtcca gtttgttaac agcacctgaa tccctatacc
     9961 gcacaccaca gttaagaaaa gaattgctgt cacgtaaatc caaaattgca gattttcagc
    10021 cagcgcgccg aaagatatat ccggattaaa aaacaggtac cacaaaacga caatgctcaa
    10081 cacatgcaac aactgatcca tcaaaaacca cccggtttta ttgttttttt tctgaaaaaa
    10141 caattttacc atgtctatcc ccacatgaac aatggtaatt gaaagcgcca cagcccaatg
    10201 ccgtaaatcc catagaacca gccataccaa cgcaccgtga atgaggccgt gcaaatataa
    10261 cttaagggat tttgcgcctt gtttttcttt ttcctcaacc cacgattttg gctgaagaat
    10321 gaaatcaccg gccagatgca ttaaaataag cttgacaagt acaatcataa aagcaacatt
    10381 tccaatttgc gccgatacat ctgctccatg gacaatactt catctatatt ggcccttttc
    10441 catcttccgc ttacggaatt ttgttttatc cccagccgcc ttccaatctc gttttgggta
    10501 taattgggat gttccaatgc cacctccacc aactcagcag aagcgaccga ccatttatcc
    10561 ataaaaactg aggccaacct gataaaaaga ttcatttccc cgtcaaaatc cggccagggg
    10621 cttttaacaa gcaaattgaa tttttctttt tccagttctt caaatttttc tccagcattt
    10681 ataaacgctt ctccgttact ttccgttatc cggtcgcccg aataactctt ccctcctatg
    10741 cctattgcca ttctcacatc aataaggctt ttatttgttt catcagaaac tatcttcttt
    10801 attaatgctt tcacccgtaa cgc
//
LOCUS       DAOSDR010000046        10451 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrimonas sp. isolate AMR_MDS_1826
            NODE_2422_length_10451_cov_4.598115, whole genome shotgun sequence.
ACCESSION   DAOSDR010000046 DAOSDR010000000
VERSION     DAOSDR010000046.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863355
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrimonas sp. (wastewater metagenome)
  ORGANISM  Petrimonas sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Dysgonomonadaceae; Petrimonas.
REFERENCE   1  (bases 1 to 10451)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10451)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:10:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,720
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 35
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10451
                     /organism="Petrimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2422_length_10451_cov_4.598115"
                     /isolate="AMR_MDS_1826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2023866"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1197)
                     /locus_tag="PLI30_03050"
     CDS             complement(<1..1197)
                     /locus_tag="PLI30_03050"
                     /inference="COORDINATES: protein motif:HMM:NF024581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HOI78564.1"
                     /translation="MRIALAIACFIVISLLFLDISGVLHKNFAFLAEFQFIPALLALN
                     VVVLVVLAVLTLVFGRIYCSVICPTGVYQDIVSYFARRIKSKKTRRYSFSAAKTVLRI
                     VVLAVFIVGVVAGIGIVSAILDPYGAYGRMVTELVSPVYGAVNNLFAKVLGVQHYTFA
                     PTEIWIKSIFSLVLASLTLLIIGFLAWRSGRTYCNTFCPVGTILGYLSKCSILKLRID
                     KTSCTMCGRCSRDCKASCIDFKNHTIDYTRCVTCFNCIESCKDGSMTYSIKKAAKKQP
                     LEIVGVSSENNSRRQFFITSLLMVASATGLKAQEKKRSMIKFPAVRKDRYKRSIPVSP
                     PGSLSHDNLNDKCTACLLCVNQCPDKIIRPSVNEYGWTAVMQPTLSFERGYCRTDCTV
                     CSDVCPT"
     gene            complement(1214..2599)
                     /locus_tag="PLI30_03055"
     CDS             complement(1214..2599)
                     /locus_tag="PLI30_03055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009017539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HOI78565.1"
                     /translation="MYAMKENDGKQKISRRTALKYMGLGAAVIPASLYLGGCTKKTDK
                     SQPVPDLTNIPTDKMTYTALSKTGDKISLLGFGTMRYPTVERETPEGIRKFIDEEKAG
                     QLVDYAIAHGINYFDTAWMYHQGESEIVTGKLLKKYPRNSFYLATKLPGSVKSREDAI
                     ATYHKQLEKLQVDYFDYYHLHSLSSDVMYERIYKEWGMLDFLLNEKKEGRIRNLGWSF
                     HGDQPLFDKVLAEPVDWDFVMIQMNYFDWKYGRVPAEYMYNRLVERNIPVMIMEPLLG
                     SRLAKVSRAVSEMMQEERSGDTPAQWAFRFVGSHPQVMVVLSGMTLMEHLQENIKTYS
                     PLVPVTDKQKDMLAKAAEIIRTYKIIPCTDCKYCVPCPYGVDIPGILLYYNKATWDSN
                     LPDLEGQRDAEFERASRAFLVDYNRTIPELEQANHCINCGECEPTCPQNIKIPTDLLK
                     IDNLVQQLKTT"
     gene            2890..3960
                     /locus_tag="PLI30_03060"
     CDS             2890..3960
                     /locus_tag="PLI30_03060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019537993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HOI78566.1"
                     /translation="MRKVLVDLSALNNIYSGFGQIALTYGKYFENNYKKETARYSLTL
                     LVPDDMIGKFGNEVNYLSSSKRLKRRFPFLFPKFDVWHSTHQLSKFRPFYSGTKQILT
                     FHDLNYLYERKGFSRYRKHRQIQGKIDRANQLVCISHFTKEEVERNLNLNGKKCSVIY
                     NYVEPFNESLASKPSIDIKTPFFFSIGALRKKKNFHVLLDLMKLYPEKHLYVAGTEAK
                     KKKKNAYALMMKERIKNEKITNVTLLGGILHKEKIWMYKNCEAFLFPSLFEGFGLPVI
                     EAMQFGKPVFSSAETSLKEIGGQFAYFWDHFDAYSMKSLIDSNLEEFYNDKEWMRKEK
                     QYAESFSTNNHFEEYEKLYESI"
     gene            3935..4891
                     /locus_tag="PLI30_03065"
     CDS             3935..4891
                     /locus_tag="PLI30_03065"
                     /inference="COORDINATES: protein motif:HMM:NF022822.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stealth CR1 domain-containing protein"
                     /protein_id="HOI78567.1"
                     /translation="MKSYMSPYEIDLVYLWVDGSDPEWLAKKREYLENKTGLNIEATS
                     KARIADNDELRYSLRSAEKYAPWIRKVFIVTDEQKPKWLNLKNEKVEIIDIRALIPPE
                     ALPCYNSVIIEHFLYKIPGLSEHFLYANDDMFFNAEIGPEFFFNSEGMPIVRLQRAFL
                     GKWMSRIRGLFNIPTNIYRKTIDRAAGLIKEKFGKYYSGTPHHNIDSYLKTDYSNVVE
                     KDFRNEILSTLINHLRSEHDIQRIVYLYYALVKKRGVLRYVSRKESCRIRLQKPDFMD
                     YIMSYNPVLFCLNDTHRATDKDRERVKPFLEALFPEKSGYEL"
     gene            complement(4905..5897)
                     /locus_tag="PLI30_03070"
     CDS             complement(4905..5897)
                     /locus_tag="PLI30_03070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019537990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stealth CR1 domain-containing protein"
                     /protein_id="HOI78568.1"
                     /translation="MEIDFVITWVDMNDPRWQKDFAIYSGKIDNTVNELSEARFRDYG
                     LLKYWFRGIEKFTPWVRKIHFVTCGQKPEWLNENHSKLHIVNHEDFIPEQYLPVFNSN
                     LIEIYLHKIPDLAEQFVYFNDDFFVTDHTPATRFFENGLPKDIATFRTNFGRSQFGKM
                     LKNNIRLINKFFDKKEVLKRDYDKWFHESYGKRRRLAYLLKPYNKFVTLRTPHNAQPF
                     LKSTFHEVWDKCGKELTEMSKNRFRSSSDLTPELFKTWQICTSKFLPYNTYQDTKMFP
                     LILKSKKAIRAVREQKYKLVCLNDNIHIRNYDKMLKELKASFESILPEKSSFEL"
     gene            complement(5984..6490)
                     /gene="purE"
                     /locus_tag="PLI30_03075"
     CDS             complement(5984..6490)
                     /gene="purE"
                     /locus_tag="PLI30_03075"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005781966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HOI78569.1"
                     /translation="MDPIISIIMGSTSDLPVMEKAACFFDEMEVPFEINALSAHRTPE
                     AVEQFARNAEKRGIKVIIAAAGMAAHLPGVIASMTTLPVIGVPIKASLDGMDALLAIV
                     QMPPGIPVATVGINGSLNAAILALQMIATGDESLRQKLATYKEDLKSKITQANEELAG
                     IKFKFKTN"
     gene            complement(6495..6875)
                     /gene="gcvH"
                     /locus_tag="PLI30_03080"
     CDS             complement(6495..6875)
                     /gene="gcvH"
                     /locus_tag="PLI30_03080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008146565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HOI78570.1"
                     /translation="MNFPENLKYSSDHEWVKVDGNEAYIGISDFAQSELGEIVFVDIT
                     TEGEKVDKGAVFGSIEAVKTVSDLLMPITGEVLEVNPKLDDQPELVNQDPYGEGWIVK
                     VTIEEEADLNELLSSDAYKALIGQ"
     gene            complement(6973..7452)
                     /locus_tag="PLI30_03085"
     CDS             complement(6973..7452)
                     /locus_tag="PLI30_03085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI78571.1"
                     /translation="MILFSFAVPGILIYAVYKLKLISDLSLKIRKERFIPYSIVLVSY
                     LVMIFYYYRIGMPGWFLMLVASSIAVILIAIFITIWWKISAHMFGIGGLLGGVMSVSY
                     FIEKSNPFYLFMALFAVAGLVGVSRLILRRHTFGQVVGGFFLGFIISFLFVWIGTHI"
     gene            complement(7617..9068)
                     /gene="rpoN"
                     /locus_tag="PLI30_03090"
     CDS             complement(7617..9068)
                     /gene="rpoN"
                     /locus_tag="PLI30_03090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006841635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HOI78572.1"
                     /translation="MALRQQQELKQTQKLSPLQMQVIKLVELNSVELEDKIKQELEDN
                     PALEEGNEELNPESSDEFPESEDNLSEEDIVLGDYFSEEDIPDYQLNKSHRKSEPDFI
                     EATYSDDKSLNEILLEQIGLRQLNARDQKIAEYIIGNLDENGYLQRSLQAISDDLLFQ
                     QNLDVPETNLQSILEIIQDLDPAGVGASDLQECLRLQLQRNEPTKGNRLALEIISNYF
                     EEFSRKHYEKIMRQLNISQDDLRDAIHEIVSLNPKPGSALGDSIEMAMSNITPDFIVE
                     SYNGEISMYLNNKNIPELKINRDFSEMLQGYAQNKESMSNDAKQAVLFMKQKVDSAKW
                     FIDAVRQRQNTLQRTMEAIVNFQYDFFVTEDETMLKPMILKDIAQKTGFDISTISRVS
                     NSKYVQTNNGVYPLKFFFSEAMQNEAGEDVSSREVKSLLKECIENENPSKPLTDEQLT
                     TLLNNKGYIIARRTVAKYREQLNIPVARLRKKI"
     gene            complement(9187..9504)
                     /locus_tag="PLI30_03095"
     CDS             complement(9187..9504)
                     /locus_tag="PLI30_03095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI78573.1"
                     /translation="MKLSAYTPIDPDFFDLFQQEIKKGSVKVFYFDADQYPHLKESNG
                     KVTAIVSKQESGQFMVFENGDEVRTDRIIVYNGKPGPAYDEYDAYALAPLTCKAGYDD
                     DDC"
     gene            9669..10064
                     /locus_tag="PLI30_03100"
     CDS             9669..10064
                     /locus_tag="PLI30_03100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI78574.1"
                     /translation="MRRLFIVLGLFLATGVFFIVNAQSNKIVLGEWKYSVPEAPFGYE
                     KGVIFLEEKDKSLSGNLVFDSGLKVKLSDVFLKSDTLKLGVYVESEYVTVVSKVKGNE
                     MEGKVDTSMGVMRLKAEKIVPQTKKKQGS"
     gene            complement(10061..>10451)
                     /locus_tag="PLI30_03105"
     CDS             complement(10061..>10451)
                     /locus_tag="PLI30_03105"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006798448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tRNA dimethylallyltransferase"
                     /protein_id="HOI78575.1"
                     /translation="KERNFNIVKIGFMRPREELYERINRRVDQMIEDGLVKEAWELYP
                     KRHLNSLNTVGYKELFDYFDGNCTLDFAIGKIKQHSRNYARKQMTWFKRDKEINWIDL
                     SNNKLDAVEKSIHLISQCNRMGKKTDG"
BASE COUNT         2972 a         2090 c         2172 g         3217 t
ORIGIN      
        1 ggtagggcaa acatctgaac aaacagtgca gtcggtacgg caataaccgc gttcaaaaga
       61 gagagtaggc tgcataaccg ccgtccatcc atattcatta accgatggac ggataatctt
      121 atcgggacat tgattgacgc acagcagaca agccgtacat ttatcgttga ggttgtcgtg
      181 acttaacgac ccgggaggag aaacaggtat ggagcgttta taccggtctt tacgaacggc
      241 aggaaattta atcatgcttc tctttttttc ttgagccttt agcccggttg cgctagcgac
      301 catcagaagt gaagttatga aaaactgtct gcgggaattg ttctccgaag agacaccgac
      361 aatctccaac ggctgttttt tagccgcctt ttttattgaa taggtcatgc tgccgtcttt
      421 gcacgattca atacagttga agcaagtaac acaacgcgtg taatcgatgg tgtgattttt
      481 aaagtcgatg cacgatgctt tgcaatctct ggagcacctg ccgcacatgg tgcaggaggt
      541 tttatcgatg cggagtttca ggatggagca cttactgagg taaccgagaa tagtacctac
      601 cggacagaag gtattgcagt aagttcgtcc gcttctccat gccagaaatc cgatgatcaa
      661 tagcgtgagt gaggccagca ccaacgagaa aatgctcttt atccagattt cggtaggggc
      721 aaatgtataa tgctgaactc ccaacacctt tgcaaaaaga ttgttaacag ctccgtaaac
      781 cggactaact aattcggtaa ccatccggcc gtatgctccg taaggatcca gaatagccga
      841 aacaataccg attccggcaa caacccctac aataaaaacg gcgagaacta ctattctcag
      901 caccgttttt gcagcggaaa aactgtagcg gcgtgttttt ttcgacttta ttcttctggc
      961 gaagtaagaa acaatatcct gatatactcc tgtaggacaa ataaccgagc agtaaatacg
     1021 tccgaaaacc agagtgagca cagcaagcac gaccagtaca actacattga gtgctaacag
     1081 ggccgggata aattgaaatt cggctaaaaa agcaaaattc ttgtgcaata cgcccgagat
     1141 atcgagaaac agcagagaaa taacaataaa acaggctatt gccaaagcaa ttctcatttt
     1201 ctttaacatg gtatcaggtg gtttttaatt gttgaacaag attatcgatt ttcaataaat
     1261 cggtggggat ttttatgttc tgtggacaag taggttcaca ttcaccgcaa ttgatgcaat
     1321 ggtttgcctg ttccagttcg ggaatagtgc ggttgtaatc taccaggaat gccctggaag
     1381 cacgttcgaa ttcagcatca cgttgccctt cgagatccgg aaggttactg tcccaggtcg
     1441 ccttgttgta ataaagcaag attccgggaa tatcaacccc atacggacaa ggaacgcaat
     1501 atttgcaatc cgtacacggt atgattttgt aggtgcggat aatttccgct gcttttgcca
     1561 acatgtcttt ctgcttatcg gtgaccggaa caaggggaga ataggttttt atgttttcct
     1621 gtaaatgttc cattaacgtc ataccgctta acactaccat tacctgtgga tgtgaaccca
     1681 caaaccggaa agcccactgc gcaggtgtgt cgccagaacg ttcttcctgc atcatttccg
     1741 atacggcacg gcttactttt gccaaacggc ttcccagtaa cggctccatg atcataacag
     1801 gaatatttcg ttccaccagc ctgttgtaca tgtattccgc aggaacacgg ccgtatttcc
     1861 agtcgaagta attcatctgg atcatcacga aatcccagtc cacaggctct gcaagcacct
     1921 tgtcaaaaag aggctgatca ccgtgaaaag accatcccag attccggatg cgtccttctt
     1981 tcttttcatt cagcagaaag tccagcatcc cccattcttt gtaaatacgt tcgtacatga
     2041 catcgctgct taagctgtgc aggtgatagt aatcaaaata atctacctga agtttctcca
     2101 attgtttgtg atacgtggca atggcatcct ctctcgattt cacactgccc ggcaattttg
     2161 ttgccaggta aaagctgtta cgggggtatt ttttcaggag tttaccggta acaatttccg
     2221 attctccctg atgatacatc catgcggtgt caaaatagtt tatgccatgt gctatggcgt
     2281 aatccaccaa ttgtcctgct ttttcttcat cgatgaattt tcttatccct tcgggtgttt
     2341 ctcgttccac ggtaggataa cgcatcgtac caaaaccgag aagcgaaatc ttgtcacccg
     2401 ttttagacaa ggctgtatat gtcattttgt cggtaggaat attggtcaaa tccggaacgg
     2461 gttggctctt gtcggttttt tttgtgcagc cgcccaaata taggcttgcc ggaataaccg
     2521 cagcacccaa tcccatgtat ttcaaagctg ttcttcgcga aattttttgt tttccgtcat
     2581 tttctttcat agcatacatg ttttgtagta tctgacaaaa atagtaaaat tcttgcaaga
     2641 ttaattgtgg acaatatcaa ttttttcttt ttttatttta ataaaaaaat agtgttgcac
     2701 attttatagt tatcggacaa tggccggtat tatcgctact ttttttatta tctttggatc
     2761 aatattttca agtggatggt attaaataga tgcatcttgc ctttctgcct gacaggatag
     2821 ggttgttgcg ttgcatatac atccttttaa tcggttgtgg ggggcggata taaattttaa
     2881 aaaaagctaa tgagaaaggt attggttgat ttatcagccc tgaataatat atacagtggg
     2941 tttggacaga tagcgttgac ctacggcaaa tattttgaga ataattataa aaaagaaact
     3001 gctcgttatt ctcttacatt gctggttcct gacgatatga taggcaagtt tggcaacgag
     3061 gtcaactacc tttcatcatc caaacgactc aaaagacggt ttccgttttt atttccaaag
     3121 tttgatgttt ggcactcgac acatcagtta agcaagttca ggcctttcta ttccggcaca
     3181 aaacagatac tgacattcca tgatctgaac tatctgtacg aaagaaaagg atttagccga
     3241 tacagaaaac accgtcaaat ccaggggaaa atagaccggg caaatcaact ggtttgtatt
     3301 tctcacttta ccaaagaaga agtagagaga aacctgaacc ttaacggaaa gaaatgcagc
     3361 gtgatttaca attacgtaga gccttttaat gaaagtctgg cttctaaacc cagtatcgat
     3421 atcaaaacgc cttttttctt ctctattggt gctttgcgga aaaagaaaaa cttccacgtc
     3481 ttgcttgatt taatgaagtt atatcctgaa aaacatcttt atgtcgcggg tactgaagcc
     3541 aaaaaaaaga aaaagaatgc gtatgccctg atgatgaaag aacgcatcaa aaatgagaaa
     3601 ataacgaacg ttaccttgct tggtggaatt ctgcataagg aaaaaatatg gatgtataag
     3661 aactgcgagg cttttctttt tccttcgttg tttgaaggct ttgggttgcc ggtaattgaa
     3721 gccatgcagt ttggaaaacc ggtgttctct tcagcggaaa caagcctgaa agaaattggt
     3781 ggacagtttg cttatttctg ggaccatttt gatgcatact ccatgaaatc tctaatagat
     3841 agtaacttgg aggaatttta taacgacaaa gaatggatgc ggaaagaaaa acaatatgcg
     3901 gaatcatttt cgactaacaa ccatttcgaa gagtatgaaa agctatatga gtccatatga
     3961 aatagatctg gtttatctgt gggtcgatgg tagtgacccg gaatggttgg ccaaaaaaag
     4021 agaatattta gagaataaaa ccggcttgaa tatcgaagcc acttcaaagg cgcgtatcgc
     4081 cgacaatgat gaattaagat attcactgcg ttctgcagag aaatatgctc cctggatccg
     4141 gaaagttttt atcgttaccg atgagcaaaa gcccaaatgg cttaatctga aaaacgaaaa
     4201 ggtggaaatc atagatatcc gtgcgcttat tccgcccgaa gccttgccat gttacaactc
     4261 ggttatcatt gaacattttt tatataaaat tcccggctta tcggagcatt ttctttacgc
     4321 taacgacgat atgtttttca atgctgaaat aggtcctgaa ttctttttta attccgaagg
     4381 aatgccgatt gtgcggttgc aacgtgcatt tttaggaaag tggatgagtc gaattagggg
     4441 gttattcaat attcctacca atatctatcg aaagaccatt gacagagcag ccggattaat
     4501 caaagagaaa tttgggaaat attattccgg aactccgcac cataatatag attcgtatct
     4561 gaaaacagat tacagcaacg tggtagaaaa agatttcagg aatgagattt tatcgaccct
     4621 gatcaatcac ctgagaagcg aacacgacat ccaacggatc gtttacttat actatgcctt
     4681 ggtaaaaaaa aggggagtgc tccgttacgt gagccgaaaa gaatcttgtc gtatccggct
     4741 gcaaaaacct gattttatgg attatatcat gtcgtataat ccagtgcttt tctgtctgaa
     4801 cgatactcat cgggcaacag ataaagatcg tgagagggtg aagccctttt tagaagctct
     4861 ctttcctgaa aaatcgggtt atgaactgta ggatagagac aatattacaa ttcaaagctc
     4921 gacttttctg ggagaatact ttcaaaagat gcttttaatt ctttgagcat tttatcgtag
     4981 ttccgaatat gtatgttgtc atttaagcag acaagtttgt atttttgttc gcggacagca
     5041 cggattgctt ttttagattt caaaataaga gggaacatct ttgtgtcttg ataggtgttg
     5101 tagggtagga attttgaagt gcaaatttgc caggtcttga ataattcagg tgtaaggtcg
     5161 gaagaacttc tgaacctgtt tttcgacatt tcggtcaatt cctttccaca tttatcccaa
     5221 acttcatgaa acgtagattt tagaaaaggc tgcgcattgt gcggggttcg cagtgtcaca
     5281 aacttattgt atggttttag cagatatgct aaccttctcc ttttgccgta cgattcatga
     5341 aaccatttat cgtaatccct ttttaaaacc tcttttttat caaaaaactt attgatcagt
     5401 ctgatgttat tcttcagcat cttaccaaac tgagaacgtc caaagttagt gcgaaaagtg
     5461 gcaatgtctt taggaagtcc attttcgaag aaacgagttg caggggtatg gtctgttaca
     5521 aaaaaatcat cgttaaaata gacaaattgt tccgctaaat cggggatttt atgcaaataa
     5581 atttctatca aatttgaatt gaatacaggt agatattgtt caggaataaa atcttcgtga
     5641 ttaactatat gcagctttga atgattctca tttagccatt ctggtttttg tccgcaggtg
     5701 acaaaatgaa tttttcgtac ccaaggggta aatttttcta ttcccctgaa ccagtatttt
     5761 agtagcccat agtctctgaa tctagcttct gacaactcat ttaccgtgtt gtctattttc
     5821 cccgaataga tggcaaaatc tttttgccat ctcgggtcat tcatatctac ccaggttatt
     5881 acaaaatcaa tttccacttc tttgttttaa gaattcaggc gaaagaataa aaaataccaa
     5941 caaatcgtga ggttgttgga atagttttct gctgacagtt tatttaattt gttttgaatt
     6001 taaatttgat gcctgccagt tcttcgttag cttgagtgat tttactcttc aggtcctctt
     6061 tgtaggttgc caatttttgc cggagtgatt catctccagt tgcgatcatc tgcaacgcaa
     6121 gaatagcggc gttaagtgat ccgttaatgc caacagtggc aaccgggatt cccggaggca
     6181 tttgcacgat ggcaagcagt gcgtccatac cgtccagtga agcttttata gggacgccga
     6241 taacgggaag tgtggtcatt gacgcaatta cacccggcaa atgggccgcc atcccagctg
     6301 ctgctattat aacctttatt ccccgttttt cggcattcct ggcaaattgc tcaacagctt
     6361 cgggtgtgcg atgtgcagaa agtgcgttta tttcgaacgg aacttccatt tcgtcaaaaa
     6421 aacaggcagc cttttccatg acgggtaagt cggaagtact gcccattatt atacttatta
     6481 ttggatccat ctgtttactg tcctatgagg gctttgtacg catcacttga gagaagctca
     6541 ttgagatcgg cttcctcttc gatagttact tttacgatcc atccttctcc gtagggatct
     6601 tggtttacca gttcaggttg gtcatccagc ttgggattga cttcaagtac ctcgccggtt
     6661 atgggcatca aaagatcaga aacggtcttt actgcctcaa tactgccaaa aacggctcct
     6721 ttgtcaacct tttctccttc tgttgtgata tcaacaaaaa caatttctcc cagttcgctt
     6781 tgtgcgaaat cggaaatgcc gatgtaagcc tcgtttccgt caactttcac ccattcatgg
     6841 tcgcttgaat actttaaatt ttcaggaaaa ttcatagaag ttattttttt aatgtgagtg
     6901 gttttaattg tagggtaaaa atactatata ttttctttac tgcaaaatct ttttaagtaa
     6961 agggatacag cactaaatat gcgttccgat ccatacaaag agaaaagaaa tgataaatcc
     7021 gaggaaaaac cctccgacaa cctgtccgaa agtatgtctt cgtaatatta atctcgatac
     7081 gccaaccaat ccggctactg cgaaaagtgc catgaacagg taaaagggat tggatttttc
     7141 aatgaaataa cttaccgaca ttaccccccc cagcaaaccg ccgataccga acatatgagc
     7201 gctaattttc caccaaatgg taatgaatat agcgatgagg ataaccgcaa tggaagaagc
     7261 aacaagcata agaaaccatc caggcattcc aatccggtaa tagtaaaata tcataacgag
     7321 gtaagaaacc aacacaatgg aataggggat aaaacgttcc tttcggatct tgagtgacag
     7381 gtcggaaatt aatttcaatt tgtataccgc gtagataagg ataccgggaa ccgcaaatga
     7441 aaaaagaatc acaggtgcaa ttattgtcca aaactgattg gcaaacaata atttgaaatg
     7501 cgtgtaaaca aaaagcagta aaatactgta tacaggcatt aataacggct ggaaaactga
     7561 cgatataatt cgggcaaatt gattcattgc tgggttatgc ttttgaaacg gcaaagttaa
     7621 attttttttc ttaaacgtgc cacaggaata ttcaactgtt cgcgatattt tgcgactgtt
     7681 cgacgggcaa tgatgtaccc tttattgttc aacagtgtgg tcagctgctc gtcggtaagc
     7741 ggtttggagg gattctcgtt ttcaatgcac tccttcagga gagattttac ttcgcgagac
     7801 gatacatctt ctcctgcttc gttttgcatc gcttcggaaa agaaaaactt cagtggataa
     7861 acgccgttat tggtttgtac gtatttactg ttgcttaccc gggatatggt ggatatgtcg
     7921 aaccctgtct tttgggcaat gtctttaagg atcatgggtt tgagcattgt ctcgtcttct
     7981 gtgacgaaaa aatcgtattg aaaatttaca atggcctcca tggttctctg cagcgtgttt
     8041 tgccgttgtc ggactgcatc gataaaccat tttgccgaat ctaccttttg tttcataaac
     8101 aaaacggctt gcttggcatc gttgctcata ctctctttgt tttgagcgta tccttgcagc
     8161 atttcggaaa agtcgcggtt tatttttaac tccgggatat ttttgttgtt caggtacatg
     8221 ctgatttcac cgttgtaaga ctccacgata aagtcagggg taatgttgct catggccatt
     8281 tctattgaat cacccaaagc actgccgggc ttggggttga gtgaaactat ctcatggatg
     8341 gcatcgcgta agtcgtcttg tgaaatattt agttgacgca tgattttctc gtagtgttta
     8401 cgcgaaaact cttcgaaata attggaaata atttccagcg ccaacctatt tccctttgta
     8461 ggctcgttcc gttgcaactg aagcctgagg cattcctgca aatcacttgc tccaacacct
     8521 gcggggtcga gatcctggat tatttccagg atggattgta aattcgtttc cggaacatcc
     8581 aggttttgtt gaaacaacag gtcatcggat atggcttgca ggcttcgttg cagatacccg
     8641 ttctcatcaa ggttaccgat aatgtactcg gcaattttct gatcccgggc attcagttgg
     8701 cgtaaaccaa tctgttccag cagaatttca ttgaggcttt tatcatccga gtaagttgct
     8761 tcgataaaat cgggttcgct ttttctgtgg gatttgttta actggtaatc cgggatatct
     8821 tcttcggaaa aataatcacc caacacaata tcctcttccg acaagttatc ttcactttcc
     8881 ggaaactcat cggaactttc cggattaagc tcttcatttc cttcttccaa cgcaggatta
     8941 tcttccagct cctgctttat tttgtcttcc agctctacag aattcaattc aaccagtttt
     9001 atcacctgca tctgtagagg cgataacttt tgagtttgtt tgagttcctg ttgttgccta
     9061 agtgccataa atgacttcta atcctatttt gagaaaacaa agatagctaa ataacctttt
     9121 ttgttattca acttaactat ttttttgtca ataccgttga gaggaatcgg cctgccaagc
     9181 gtagaactaa caatcgtcat cgtcatatcc tgctttacag gtaagcggag cgagtgcgta
     9241 agcgtcgtac tcatcatagg ccggaccggg tttaccgttg taaacaatga tccggtcggt
     9301 cctcacctca tcaccgtttt cgaaaaccat aaattgtccc gattcttgtt tactaacgat
     9361 tgctgttact tttccgttcg attctttcag atgcggatat tgatccgcgt cgaaataaaa
     9421 cacctttacc gaaccttttt tgatttcttg ttgaaacaga tcaaaaaaat cgggatcaat
     9481 gggggtgtat gcagacagtt tcatttcttt attctccttt catggattaa acagtgcaat
     9541 atgggtttga gttcattcag ttctttatgt atgctttata tatttttatt ttaagagttt
     9601 tcttgtgcga aaaatttata tctttggtca tataaattca aaaatctttc tttcaaaaat
     9661 caatagttat gagaagatta tttatcgttt tggggttgtt tttggcaaca ggagtctttt
     9721 ttattgtaaa tgcacaaagt aataaaatcg tactgggaga gtggaagtac agtgtgccgg
     9781 aagcaccttt cggatacgaa aaaggggtga ttttccttga agaaaaggat aagtcattat
     9841 cgggcaatct cgttttcgat tcaggtctta aggtgaagtt atccgatgtt tttctcaaat
     9901 ccgacacatt aaaattgggt gtttatgttg aatcggagta tgttactgta gtttcgaagg
     9961 tcaaaggcaa tgaaatggag ggtaaggtag atacttccat gggggttatg aggctgaaag
    10021 ccgagaaaat tgtaccgcag accaaaaaga aacagggaag ttaaccgtcg gtttttttcc
    10081 ccatccgatt gcattgggaa atcaggtgaa tgcttttttc tactgcatcc aatttattat
    10141 tggaaaggtc gatccagtta atctccttgt cccttttgaa ccacgtcatt tgtttgcggg
    10201 catagtttcg agaatgttgt tttattttcc cgatggcaaa atcaagggtg caattcccgt
    10261 cgaaataatc gaaaagttct ttgtacccca cggtatttaa agaattcagg tgtcgtttcg
    10321 gataaagttc ccaggcttct ttcactaagc cgtcctcaat catttggtca acgcgacggt
    10381 tgatacgttc atataattct tcccgcgggc gcataaatcc gatttttaca atgttaaaat
    10441 tcctttcttt t
//
LOCUS       DAOSDR010000047        10441 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrimonas sp. isolate AMR_MDS_1826
            NODE_2429_length_10441_cov_4.355286, whole genome shotgun sequence.
ACCESSION   DAOSDR010000047 DAOSDR010000000
VERSION     DAOSDR010000047.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863355
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrimonas sp. (wastewater metagenome)
  ORGANISM  Petrimonas sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Dysgonomonadaceae; Petrimonas.
REFERENCE   1  (bases 1 to 10441)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10441)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:10:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,720
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 35
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10441
                     /organism="Petrimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2429_length_10441_cov_4.355286"
                     /isolate="AMR_MDS_1826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2023866"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..433)
                     /locus_tag="PLI30_03110"
     CDS             complement(<1..433)
                     /locus_tag="PLI30_03110"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOI78576.1"
                     /translation="MKIAVLSDNRAEGGSLEPEHGLSLYLETAQFSCLLDVGASDRFI
                     RNAEKMNIDIRAVDFVFISHGHMDHIGGLPAFLEFNQTAPVYLSENLLSQIYYSERNG
                     FHRISLDVDLTPYEDRFVNVGPGIVFSHELTLSMASSRQYPR"
     gene            complement(430..846)
                     /locus_tag="PLI30_03115"
     CDS             complement(430..846)
                     /locus_tag="PLI30_03115"
                     /inference="COORDINATES: protein motif:HMM:NF014101.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF134 domain-containing protein"
                     /protein_id="HOI78577.1"
                     /translation="MSRPKQNRKISIPPLMQGFKPFGIPRKMLKEVTLLYDEYEAIRL
                     VDYEGLHQEQAARQMNVSRPTLTRIYEQARKTLAQALVEGNMVNILGGNVQFDREWFR
                     CRRCYKLIEGRENHVRCKDCKTYGNDELMPINAEGK"
     gene            complement(861..1046)
                     /locus_tag="PLI30_03120"
     CDS             complement(861..1046)
                     /locus_tag="PLI30_03120"
                     /inference="COORDINATES: protein motif:HMM:NF036294.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5320 domain-containing protein"
                     /protein_id="HOI78578.1"
                     /translation="MPKMDGSGPVGQGCGTGRKLGNCSSLSPEEKAKGLGLGMGKRRK
                     AGGGDGNRKRHQEGNQF"
     gene            complement(1334..2485)
                     /locus_tag="PLI30_03125"
     CDS             complement(1334..2485)
                     /locus_tag="PLI30_03125"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HOI78579.1"
                     /translation="MKKFSLLLITALFAFGMSAQEVQNVSNGTVYLKDKASDHWYIGL
                     GGGTNLYLTKAENDADASFGDRLGWMGQLEVGRWNSPVWGTRLVIDGGHIKHVANVPF
                     GPEQNWLGGHLDIMWSMTNALGGYNADRVWNLAPYLGIGYMYGLDEDWKKPNPNGETL
                     KFQNQSLTYNVGLINNFQISRSVALFLELGWRVMQESFDGSPTSGDYDYDSMLTGTAG
                     IKFGLGKQDFTPAELMDYNLINDLNSQINRLRAENEELRKRPESCPDCPEVAPAVTES
                     VYVPNVVFFRIDSYKIDKGQQVSVYNTAEYLKANPNATVKIVAYADRQTGTPAYNLAL
                     SEKRAKAVADALTSEYGIDSSRISVDWKGDTEQPYAENDWNRVAIFFAE"
     gene            complement(2760..3620)
                     /locus_tag="PLI30_03130"
     CDS             complement(2760..3620)
                     /locus_tag="PLI30_03130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008625412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD/SigA"
                     /protein_id="HOI78580.1"
                     /translation="MRQLKITKSITNRESASLDKYLQEIGREDLITVEEEVELAQAIK
                     KGDRKALEKLTRANLRFVVSVAKQYQNQGLSLPDLINEGNLGLIKAAEKFDETRGFKF
                     ISYAVWWIRQSILQALAEQSRIVRLPLNQVGSLNKISKAFQKFEQENERRPSAEELAL
                     ELDLSVDKVTDSLKVSGRHISMDAPFVEGEDNSLLDVLVNDDAPIADRTLINESLQKE
                     IDRALATLTDRESDIIKMFFGIGCQEMTLEEIGDKFQLTRERVRQIKEKAIRRLRQDS
                     RSKLLKSYLG"
     gene            complement(3826..5349)
                     /locus_tag="PLI30_03135"
     CDS             complement(3826..5349)
                     /locus_tag="PLI30_03135"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007658645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Do family serine endopeptidase"
                     /protein_id="HOI78581.1"
                     /translation="MNTGNVKNIVTIVIVAILSSVITLFGYNTVTKNNSKGILNSSGS
                     VRQSEYADSFDQDKNVRLANLTTQQGYPDFTEAAAKSVNGVVHVKVKSVSQQQYMNPF
                     DFFFGFGERNTQPREQIGFGSGVIISKDGYIITNNHVVEGATEVTVSLNDNREMTAKV
                     IGADPQTDIALIKIEGDDFPYLTFGNSDALEVGEWVLAVGNPFNLTSTVTAGIVSAKN
                     RGNIVGGNLNIQSFIQVDAAVNRGNSGGALVNTRGELVGINTAIFSQSGDFNGLAFAV
                     PISIAGKVAADLKQFGTVQRAVLGIQVPNIEMVRRQDPDKASELSQIVGVLVEDFGDR
                     SAAKAAGLEKGDIIKAINNVQIRNFAGLQDQLSRYRPGDKVKVTVDRNGKEHSYTVEL
                     KNDAGNTEIQRSSDSMSLLGATFSDIPNSRKQQLGINSGVEVSSVDNSGLFRKEGINK
                     GFIIMRVNNTPVNSGDEIAKIVSSVSRSSQDKVILIAGFYPNGRTQYIAIDLSQGLK"
     gene            complement(5550..6725)
                     /locus_tag="PLI30_03140"
     CDS             complement(5550..6725)
                     /locus_tag="PLI30_03140"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOI78582.1"
                     /translation="MDLPYIFNFNFPLPFWIISGAVLLFFSIQLVYYLLVYRKPYVYE
                     QKRNKSLPLSENLPSVSVVIASKNESENLEKYLPAILEQDYPDFEVIVVNMGSTDETD
                     VLLKGLNQKYPHLYHTYVPAEAEDVNGKKLALTLGIKAAKNDILLFTEAYCVPASDHW
                     IREFGREFSKGRDIVLGFCKLQVGRKVAMRQFILYDNLIHGLKYLSLAVLGKPFMGIG
                     RNLAYRKEIFFEEKGFSSVLNIDEGEDDLFINKIAGKKSVGVVVSPESMTQSDVVNNF
                     FTWRALKSKYLYTKQFYKGVSSFVFGFETFSKYLFYLSVVSGITYGMVFGNYPLIALS
                     VFFLIVRFVVQLYVIGKSSRLFNAGKYHVNLFFFDLFQPFNNFKFRKYANKRNRLRK"
     gene            complement(6728..7885)
                     /gene="lysA"
                     /locus_tag="PLI30_03145"
     CDS             complement(6728..7885)
                     /gene="lysA"
                     /locus_tag="PLI30_03145"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010802325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HOI78583.1"
                     /translation="MIKGQFPVEKFHSLETPFYYYDMDLLRQTLDAVKKEGEGKNYIV
                     HYALKANANPRILREIASYGFGADCVSGNEILKALDCGFPASKIAFAGVGKTDKEIKI
                     GLDNGIFCFNVESLPEMEAIDRLASETGNVAPVALRINPNVDAHTHRYITTGLEENKF
                     GLNESDLPKAIDLIEKSKHLKLIGMHFHIGSQITDLSSFEDLCVKAVELQNWFSQRGI
                     SFEVLNVGGGLGINYHHPNHCPVADFEAYFRLFAKYLKLQENQTLHFELGRSFVAPCG
                     SLIARTTYVKEGTTKKFLITDAGMSDLIRPALYQAFHHIENISSDKEYETYDVVGPVC
                     ESSDVFGEAVLLNQSRRGDFIAIRSAGAYGETMASMYNCRALPRSYFSDSL"
     gene            complement(7972..8886)
                     /gene="miaA"
                     /locus_tag="PLI30_03150"
     CDS             complement(7972..8886)
                     /gene="miaA"
                     /locus_tag="PLI30_03150"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008150531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HOI78584.1"
                     /translation="MKKLITVLGPTASGKTTFAAHLASRLDGEIISADSRQVYRGMDI
                     GTGKDLSDYRIGDNPVPYHLIDIRDAGDKYTLFNYQHDFHKVYSDVLSRNKTAILCGG
                     TGLYIESVLKGYRLPDVPENAVLRKRLETKSLDELTRILSSYKPLHNTTDTDTKKRAI
                     RAIEIADFQCKHPASELDYPPVESVIIGLDIDRESRRQKISSRLKKRLDEGMVAEVQS
                     LLNKGVSPDDLIYYGLEYKFVTLYVTGKINFEEMFSELEIAIHQFAKRQMTWFRGMER
                     RGFTIHWLDFLLPVDEKIEKALVLIRNS"
     gene            complement(8886..9653)
                     /locus_tag="PLI30_03155"
     CDS             complement(8886..9653)
                     /locus_tag="PLI30_03155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006798069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HOI78585.1"
                     /translation="MKSRLAFIVLFFLLSLGISAQDVPDPIYPYRLVNDFANIFSAAE
                     KQALEQRLLAYNDSTSTQIYVVAVNDLGGYPASDYAFVLGEKWGIGQKGKDNGLLILI
                     KPKTADSRGQAFIATGYGLEGSINDAFAGRIVRNNMIPYFQENDYFGGVNAAVDAIIA
                     RLSGEFDADAGEPEGVPIWAIVLLFVVVFILFSLFSRGGDQHIDGDGHHRAGGGPLFF
                     PPFSGGRSSGWGGGFGGGGFGGGGGGSFGGGGAGGSW"
     gene            complement(9643..10068)
                     /locus_tag="PLI30_03160"
     CDS             complement(9643..10068)
                     /locus_tag="PLI30_03160"
                     /inference="COORDINATES: protein motif:HMM:NF016424.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HOI78586.1"
                     /translation="MLTPEELNRVVESIRAAEGRTSAEIRVCIAKKCKENPLDAAYKK
                     FKQLKMDTTALRNSVLIYVAPDNHKAAVVGDSGIDEAAQEGFWDSVLEEMFSFFKNGR
                     ICEGICRGVGKVGELVNSRYPVSENDVNELCDDVIWDEE"
     gene            complement(10085..>10441)
                     /locus_tag="PLI30_03165"
     CDS             complement(10085..>10441)
                     /locus_tag="PLI30_03165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007215350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HOI78587.1"
                     /translation="RAKATQMTVDPESLTEESIQQYQQAQSQLGSAIGRLLLIQENYP
                     ELKANQNFLALQDELAGTENRISVERNKFNQTAQDFNAYIRQFPRVIYAGWFGFKPKG
                     YFQSSPGAETAPQVQF"
BASE COUNT         2931 a         2556 c         1976 g         2978 t
ORIGIN      
        1 gacggggata ctgacgggaa gaggccatag acaaggtaag ctcatgactg aaaacaattc
       61 ctggccccac attgacaaaa cggtcttcat aaggtgtaag atccacatcg aggcttatcc
      121 ggtgaaatcc gtttctttcg gagtaataga tttgactcaa caggttttct gagagataaa
      181 caggcgccgt ttggttaaac tccaggaagg caggcaaacc accgatatga tccatatgcc
      241 cgtgagagat aaatacaaaa tctactgcac ggatatcaat attcatcttt tcagcattcc
      301 ttataaaccg gtcagaagct cccacatcaa gcaaacagct gaattgcgca gtttccagat
      361 agagggaaag cccgtgttcc ggctccagcg acccaccctc agcccgatta tcagacagca
      421 ccgcaatttt catttccctt ctgcatttat tggcattaac tcgtcgtttc catatgtttt
      481 gcagtccttg caacgaacat gattctctct cccttcgatc aatttgtaac aacgtctaca
      541 gcgaaaccat tccctatcga actgcacatt gccacccaaa atattgacca tgttcccttc
      601 caccaaagcc tgagcaagag tcttacgggc ctgttcgtaa atacgcgtca aggtaggccg
      661 ggatacgttc atctgcctcg ctgcctgttc ctgatgcaag ccttcgtagt ctaccaaacg
      721 gatggcctca tattcatcat acaataaagt aacctccttc aacattttcc gagggatgcc
      781 aaacggctta aatccctgca tcaggggagg aatggatatt tttcggtttt gtttgggacg
      841 cgacatatta atcttctttt ttaaaattga tttccttcct ggtgacgttt tctattaccg
      901 tctcctccgc ctgccttgcg gcgcttcccc attccaagtc ccaacccttt cgctttttct
      961 tcaggtgaaa gcgaactaca attacctaat tttcgtcctg ttccacagcc ttgtccgaca
     1021 ggaccgctac cgtccatttt tggcatgaca tcagtttttt ttacgtgttt taaacttact
     1081 tatcaagctt ttcagctcct tatttataaa tttatcaggt gtaaagataa taatagtttt
     1141 gaacatatgc tcaccaaaag atctgttctt taaaaaaatg agaaataaat ataagaaata
     1201 caaagtaggg agagcgtttt cataaagaag aaaagccgga tgtccggcgt aaaccgagtc
     1261 ccggctcagg ataatacaat aaaaaaagca tctcaaacga gatgcttttt ttagtatatc
     1321 ttggctttct gaattattca gcaaagaaga tagctacgcg gttccagtcg ttttctgcgt
     1381 aaggctgttc agtatcacct ttccagtcaa cagagatgcg gctgctgtca attccgtatt
     1441 ccgaagtcaa tgcgtcagca acggctttag cacgtttttc agaaagagcc aggttgtaag
     1501 caggtgtacc tgtttgtctg tcggcgtaag caacgatctt aacggttgcg ttaggattag
     1561 ctttcaagta ttcagcagtg ttgtaaacgc taacttgctg gcctttatca atcttataag
     1621 agtcgatgcg gaagaatact acgttaggaa cgtaaacact ttcagtaact gcaggagcaa
     1681 cttcaggaca atccgggcaa gattccggtc tttttctgag ttcttcgttt tctgcgcgaa
     1741 gtctgttgat ctggctgttc aaatcgttga tcaggttgta atccatcaac tcagcaggcg
     1801 tgaaatcttg tttgccaagg ccgaatttga ttcctgcggt tccggtaagc atgctgtcgt
     1861 agtcgtaatc tccgcttgtt gggcttccgt caaaagattc ctgcattact ctccatccca
     1921 attcgaggaa taaagccacg cttcttgaaa tctggaagtt gttgatcaaa ccaacgttgt
     1981 aagtaaggga ttgattttgg aattttaatg tttcaccgtt tggattcggt ttcttccaat
     2041 cttcatccaa tccatacatg taaccaatac caagataggg agcaaggttc catactctat
     2101 cagcattgta accacccaaa gcattggtca tgctccacat tatatcaaga tgaccgccca
     2161 accaattttg ttcaggtccg aaaggtacgt tagctacgtg tttaatatga ccgccgtcaa
     2221 taaccaggcg ggtaccccat accggagaat tccatcttcc aacttcaagt tgtcccatcc
     2281 atcctaaacg atcgccgaaa cttgcatctg catcgttctc tgctttagta agatagaggt
     2341 ttgttccacc accaagacca atataccaat ggtcactagc tttatccttc aggtacaccg
     2401 taccgttgga aacgttttgt acttcttgtg cactcatgcc gaatgcaaaa agagccgtaa
     2461 ttaaaagtaa actaaacttt ttcataaatt taaattctaa ttaaaatttg ttttattaca
     2521 ataactcgat aattaatgtc tatagttaat actgctgagt atgatctatg ttttataaca
     2581 tttgtatttt taaaatgttc gcaaaagaat aaaaaactaa taaaattagc ttttcttaag
     2641 cgaaatcatc gcaaagataa atctaatttc caaaacacaa tcgttttttt aaggaaaaat
     2701 gataaaaatg acctgattta gggtattttt ccacattttt cattatatag acggttctat
     2761 cagcccaaat agctttttaa cagtttactc ctcgaatcct gacgaagcct tcttatggct
     2821 ttttccttta tctggcgcac acgttcgcgg gtaagttgaa atttatctcc aatctcctcc
     2881 agtgtcatct cctgacaacc tatgccaaaa aacatcttga tgatgtcgct ttcacggtcg
     2941 gtaagggtgg ccagagcacg atcaatctct ttttgcaacg attcgttaat cagtgtcctg
     3001 tctgcaatgg gcgcatcgtc gtttacgagg acatccagta gactattgtc ctcaccctca
     3061 acaaaaggag cgtccatcga tatatgcctt ccggaaacct tcagcgaatc cgtcacctta
     3121 tcaaccgaaa ggtcaagctc caaggccagt tcttcagcag aaggacgccg ctcgttctcc
     3181 tgttcaaatt tctggaaagc cttgctgatt ttgttcaaag accccacctg attaagcggc
     3241 agacgaacga ttctcgattg ctcggccaac gcctgcagga tagattgacg gatccaccac
     3301 accgcgtaac tgatgaattt aaatccccgg gtttcatcga atttctcggc agcttttatc
     3361 aacccgaggt tcccctcgtt aatcaggtcg ggaaggctaa gcccctggtt ttggtattgt
     3421 tttgccacag acacgacaaa ccgcaaatta gcccgggtca atttttcaag agctttcctg
     3481 tcaccttttt taattgcctg agccaattcc acttcttcct ccactgttat caggtcttcg
     3541 cggcctatct cctgaaggta tttgtccaaa gaagcgcttt cacgatttgt gatcgatttt
     3601 gtaatcttta attgtctcat ttattatatt ttatcctttc tacttttaaa acgggggtgc
     3661 aaagatactg tttttttttg caataagaga attttaatcc atctttatat cgtttgcttt
     3721 taacagcgaa ggatttggat actccaaacc ctttgctata caaacacact tcggtatgtt
     3781 tttgttacaa aaaaacagcc aattaaccta aattaacaca acctcctatt ttaggccttg
     3841 cgataagtct atcgcaatgt attgagttct tccgttcggg taaaaaccgg caatcaatat
     3901 aactttgtcc tggctgcttc tgctcaccga tgaaacaatt ttagcaattt cgtctccgct
     3961 gtttacaggg gtattattta cccgcatgat tataaatcct ttgtttattc cttctttcct
     4021 gaataaaccg ctgttatcca cgctcgacac ctcgacaccg ctgttaatcc caagctgttg
     4081 ttttctgctg ttggggatgt cgctgaaagt agcacccaac aaactcatac tgtccgaact
     4141 tctttgaatt tccgtatttc cggcatcgtt tttcaattca acagtatacg aatgctcttt
     4201 accatttctg tcgacagtta cctttacctt gtctccggga cgatacctgc tcaactggtc
     4261 ctgcaatcct gcaaaattac gaatttgcac gttgttaatc gctttaatga tatccccttt
     4321 ttccaggcca gcggctttag cagcacttct gtcaccaaaa tcttccacca aaactcctac
     4381 aatctgggaa agttcactgg ctttgtctgg atcctggcgt ctgaccattt caatgttcgg
     4441 cacttgtatt cccagcacgg ctctctgcac ggttccgaat tgcttcaagt ctgccgccac
     4501 tttccctgca atagagatag gtacggcaaa agccaagcca ttaaaatcac ccgattgaga
     4561 gaagatggct gtatttattc ccaccaattc acctcgggta ttgaccaatg ctcccccact
     4621 gttacctctg tttacggcag catcaacctg gataaaagat tggatattca aatttccacc
     4681 aacaatattt cccctatttt tagcactaac gatccctgcg gttacagtag aagtcaggtt
     4741 aaacggattt ccaacggcaa gaacccactc tcccacttct aaagcgtcgg aatttccgaa
     4801 agtgagataa gggaaatcat ccccttctat ttttattaag gcaatatctg tctgcggatc
     4861 cgcaccgata actttggccg tcatctccct gttgtcgttg agagaaaccg ttacttcggt
     4921 tgctccttca accacatggt tattggtgat tatatatccg tcctttgaaa taataacccc
     4981 cgacccgaat ccgatttgtt cacggggttg ggtatttctt tctccaaaac cgaagaaaaa
     5041 atcaaacgga ttcatatatt gctgttggct gacggacttc acctttacgt ggacaactcc
     5101 attcaccgac ttggcagcgg cttctgtaaa atctggatac ccttgctggg tagtcaaatt
     5161 agccaatcgc acgtttttgt cctgatcaaa agaatcagcg tattcacttt gtctgacact
     5221 cccggaagaa tttaaaattc ctttcgaatt atttttcgta acagtattgt acccaaaaag
     5281 agtaattacc gaactgagta tagcgacaat aacaattgtc acaatgtttt ttacgtttcc
     5341 agtattcata ttcttattta ttaagtttga attttaacac ttacacaaat gtagaaacaa
     5401 aaagcgtaca agagtttaac tcaaagattc ttttttgaca cttttaacta aaagaagaat
     5461 cgattaaccc gctttaaccg taaaaaaaac aatttcaaaa cttttaaatg tcagattggc
     5521 agaaaatcgt ctgaaagcca cagcatctct tattttctca atcggttcct tttgttggcg
     5581 tatttcctga atttgaagtt attgaaaggt tgaaaaagat caaaaaagaa taggttgaca
     5641 tggtattttc cggcattaaa gagccggctg cttttaccga ttacgtataa ctgaaccaca
     5701 aacctgacaa tcaggaaaaa gacacttaaa gcgatgagcg ggtaattacc aaagaccatt
     5761 ccgtacgtaa ttcctgaaac aacagagaga tagaacaagt atttcgaaaa tgtttcgaaa
     5821 ccaaaaacga aagacgatac tcctttataa aactgtttgg tgtacaaata cttggacttc
     5881 aaagccctcc aggtaaaaaa attgttcact acatcacttt gcgtcatgct ttccggcgat
     5941 accaccaccc ctacactctt cttcccggct atcttattaa tgaacaagtc atcttcaccc
     6001 tcgtcgatgt tcaaaaccga cgagaatcct ttctcctcga agaatatttc ctttctgtaa
     6061 gccaggttcc tgccgatccc cataaatggc tttccgagaa cagcgagcga caaatacttc
     6121 aacccgtgga tcaggttgtc gtaaagtatg aactgtctca ttgcaacctt cctcccaacc
     6181 tgtaatttac aaaaccccaa tacaatatcc ctacctttag agaattcacg gccaaactcc
     6241 cggatccaat gatctgaggc cggcacacag taagcttccg taaacaataa gatatcgttt
     6301 tttgccgcct tgatccctaa ggtcaatgca agtttcttcc cgttcacatc ctctgcttca
     6361 gccggcacat aggtatggta aagatggggg tatttttgat taaggccttt gagcaataca
     6421 tccgtctcat ccgtcgaccc catgtttacc acgattacct caaaatcagg ataatcctgt
     6481 tccagtatag ccggaaggta tttctccaaa ttttcagatt cattttttga agcaataacc
     6541 accgaaacgg aaggtagatt ttctgaaagc ggcagagatt tgttcctttt ctgttcgtaa
     6601 acatacggtt ttctgtaaac caacaaatag taaaccaatt gaatactaaa aaaaagcaaa
     6661 acagctcccg aaataatcca aaagggaagt ggaaaattga aattaaaaat gtacggtaaa
     6721 tccataatta caacgagtca gaaaaataag accggggcaa tgcgcggcaa ttgtacatcg
     6781 acgccatcgt ctctccgtaa gcaccggcag aacggatagc gataaaatct ccccgccggg
     6841 attgattcaa caacacagcc tccccaaaaa catcactcga ctcacaaacg gggccgacaa
     6901 catcgtaagt ttcgtactct ttgtctgaac tgatgttttc gatatgatgg aatgcctgat
     6961 agagggcagg gcggatcaaa tcggacattc cggcatccgt gatcagaaac tttttggtgg
     7021 taccctcttt cacgtatgtg gtcctcgcga taagggaccc acacggtgcg acgaaagatc
     7081 gccccaactc aaaatgaagt gtttgatttt cctgaagttt caagtacttg gcaaacaatc
     7141 tgaaataagc ttcaaaatca gcaacaggac aatgattggg atgatggtaa ttaattccca
     7201 aaccaccccc tacgttaaga acttcgaaag aaataccccg ctgagaaaac cagttctgta
     7261 attcaacggc tttcacacac aaatcctcaa acgacgataa atccgtaatc tgcgaaccga
     7321 tgtgaaaatg catcccgatc aacttcaaat gcttgctttt ctcgatcaaa tcaatagcct
     7381 tcggtaaatc cgattcgtta agcccaaact tattttcttc cagtccggtg gtgatatacc
     7441 ggtgtgtgtg agcatccaca ttcgggttga ttcgcagcgc aacaggagcc acatttcctg
     7501 tttcagaagc cagccgatcg atggcctcca tttcaggcaa tgactccaca ttaaagcaaa
     7561 aaataccatt atccaacccg atcttgatct ctttgtcggt tttccctact ccggcaaaag
     7621 ctattttcga cgcgggaaat ccgcaatcca gggctttaag tatttcgttt ccgctcacac
     7681 aatccgctcc aaaaccataa gacgctattt cgcgcaatat cctcggattg gcgtttgctt
     7741 tcaatgcgta atgaacaatg taattcttgc cttcgccctc cttttttacc gcgtcaagtg
     7801 tttgacgcag caaatccatg tcgtaataat agaatggcgt ttccaaagaa tgaaattttt
     7861 cgacgggaaa ttgacctttg atcatcgatt tttattttat agaatgtaaa aaatggtgcc
     7921 ggcaaaaata catttttttg ccgatatttc gggaatttac acggtttaaa ttcaggaatt
     7981 ccggataagg acaagcgctt tttcgatttt ttcatccacc ggcagcaaaa aatccagcca
     8041 atgaatggta aaaccgcgcc tctccatacc acggaaccag gtcatctgac gcttggcaaa
     8101 ctgatgaatg gctatttcga gttccgaaaa catctcctcg aaattaattt taccagttac
     8161 gtacagcgtt acaaacttgt attcaagtcc gtaataaata agatcatcgg gagaaacacc
     8221 cttattgagc agggactgta cctctgcaac cattccttca tccaaccttt ttttcaggcg
     8281 tgaagatatt ttttgccttc gggattcccg gtcgatgtcc agcccgatga taacactctc
     8341 aactggaggg taatcaagtt ccgaagccgg atgtttacat tggaaatccg caatttctat
     8401 agcgcggatt gcccgttttt ttgtgtcggt atcggtggta ttgtgaagcg gtttgtatga
     8461 agagagaatc cgcgtaagtt catccagcga tttagtttcc agccttttcc tcaggacggc
     8521 attctcggga acatcgggca ggcggtaccc tttcaggaca gattcaatat aaagcccggt
     8581 acctccgcac agtatcgctg tcttgttgcg cgataaaaca tcgctgtaaa ccttatggaa
     8641 atcgtgctgg taattgaaca acgtgtactt gtcgcccgca tcacgaatgt cgataaggtg
     8701 gtaagggacc ggattgtctc caatccggta atcagacaag tcttttcccg tacctatatc
     8761 cattccgcgg tatacctgac gcgaatcagc actgataatt tcgccgtcga gccgtgaggc
     8821 gagatgagct gcaaacgtag tttttccact ggccgtagga ccaagaacgg ttatgagttt
     8881 cttcatcacc agcttcctcc tgctccacca ccaccgaaac ttccgccgcc accgccacca
     8941 aagccaccgc caccgaagcc accgccccaa ccggaacttc ggcctcctga aaacggtgga
     9001 aaaaacagag ggcctccacc ggccctgtga tgtccgtcac cgtctatgtg ctgatcccca
     9061 ccccgggaga aaagagaaaa caagataaaa acaacgacaa aaagcaaaac aattgcccaa
     9121 ataggaaccc cttcaggttc accggcatct gcatcaaatt cccccgacaa ccgggcgata
     9181 attgcatcaa ctgcggcgtt tactcctcca aaatagtcgt tctcctgaaa atagggaatc
     9241 atgttgttgc gtacaatcct accggcgaaa gcatcgttga tggaaccctc cagcccataa
     9301 ccggtggcaa taaacgcctg ccccctgcta tcagcggttt taggttttat gaggatcagc
     9361 aacccgttat ctttaccctt ttgcccgata ccccactttt cacccagtac aaatgcgtaa
     9421 tccgatgccg ggtatccgcc cagatcgttc acagccacca cgtaaatctg agtggatgta
     9481 gaatcgttgt atgctagtaa ccgctgttcc aacgcctgtt tttcggcagc agaaaaaatg
     9541 ttggcaaaat catttaccag gcggtacgga taaatcggat caggaacatc ctgtgccgaa
     9601 attcccaagg acagtaaaaa aaacaataca ataaaggcaa gcctactctt catcccatat
     9661 aacatcgtcg cacaattcat ttacatcgtt ctccgaaacc ggataacggg aatttaccaa
     9721 ctctccaact ttacccacac cccgacagat cccctcgcaa atacgtccgt ttttaaaaaa
     9781 agaaaacatc tcttcaagca ccgaatccca gaagccttcc tgagctgcct catctattcc
     9841 tgagtcacct acaacggcag ccttgtgatt gtcgggggct acataaatca acaccgaatt
     9901 acgcaatgcg gtagtatcca tttttagctg tttgaacttc ttgtaagcgg catccagggg
     9961 attctcctta cattttttcg caatgcagac tctaatctct gcagaagtcc tgccctctgc
    10021 ggcccgaatg gattcaacca ccctgtttaa ttcttccgga gttaacataa tcggggcaag
    10081 gcgtttaaaa ctgaacctgg ggagccgttt ccgctccggg agaagactgg aaataacctt
    10141 ttggcttgaa accaaaccat ccggcataaa taacacgcgg gaactgacgg atataagcat
    10201 taaaatcctg agccgtttga ttaaacttgt ttctctccac cgagatgcgg ttttcggtac
    10261 ctgccaattc gtcctgaaga gccaaaaagt tttgatttgc cttcagttcg ggataatttt
    10321 cctgaataag tagcaatcgt ccgatagcag aacctagctg gctttgagct tgctggtact
    10381 gctgaatact ttcttcggtc aaactttctg gatcaactgt catctgcgtt gctttagcgc
    10441 g
//
LOCUS       DAOSDR010000048        10389 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrimonas sp. isolate AMR_MDS_1826
            NODE_2448_length_10389_cov_4.305013, whole genome shotgun sequence.
ACCESSION   DAOSDR010000048 DAOSDR010000000
VERSION     DAOSDR010000048.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863355
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrimonas sp. (wastewater metagenome)
  ORGANISM  Petrimonas sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Dysgonomonadaceae; Petrimonas.
REFERENCE   1  (bases 1 to 10389)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10389)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:10:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,720
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 35
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10389
                     /organism="Petrimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2448_length_10389_cov_4.305013"
                     /isolate="AMR_MDS_1826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2023866"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..375
                     /locus_tag="PLI30_03170"
     CDS             <1..375
                     /locus_tag="PLI30_03170"
                     /inference="COORDINATES: protein motif:HMM:NF012308.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serpin family protein"
                     /protein_id="HOI78588.1"
                     /translation="WGEIRAALHEYEVELYLPKFKTKYSKKLNEVLTNMGMGLAFSDA
                     ADFSRMSAFPARIAFVKQDTYISTDEQGTEAAAVTTVGMELTSMPAIPQKVVFNADKP
                     FIYLIQENSTGAVLFMGAVKDI"
     gene            766..3309
                     /locus_tag="PLI30_03175"
     CDS             766..3309
                     /locus_tag="PLI30_03175"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005855085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobalamin-dependent
                     ribonucleoside-diphosphate reductase"
                     /protein_id="HOI78589.1"
                     /translation="MKKEIYTFDEAYNASLEYFRGDELAAKVWVSKYALKDSEGNIYE
                     KTPEDMHWRLANEVGRIEKKYANPLSEKELFDLFDQFKYIIPQGSPMTGIGNDFQVAS
                     LSNCFVIGMGGSADSYGAIIRIDEEQVQLMKRRGGVGHDLSHIRPKGSPVKNSALTST
                     GLVPFMERYSNSTREVAQDGRRGALMLSVSIKHPDAEDFIDAKLEQGKVTGANISVKI
                     DDDFMKAASEGKPYVQKYPIDSDTPKYEKTINAQELWEKIVHNAWRSAEPGVLFWDTI
                     IRESVPDCYADLGFQTVSTNPCGEIPLCPYDSCRLLAINLYSYVMNPFKENAYFDFEL
                     FGKHVRLAQRIMDDIIDLESEKIDKILDKINSDPESEEVKSSERNLWEKIRRKTLEGR
                     RTGVGITAEGDMLAALGVRYGSEEGNDFSERVHKMVALNAYASSVEMAKERGAFEIYD
                     TEREKNNPFVNRLKKEDPQLYKDMVKHGRRNIACLTIAPTGTTSLMSQTTSGIEPVFM
                     PVYKRRRKVNPSDKDVKIDFVDENGDSWEEYVVFHHKFVNWMEANGYPTAKKYSNEEI
                     DELVAKSPYYKATSNDVDWLMKVRMQGRVQKWVDHSISVTINLPGDVTEELVGELYME
                     AWRSGCKGCTVYRDGSRSGVLITNDNKEEEKTSADCYELPQVVTSRPTELDADVIKFQ
                     NNKEKWIAFIGLLNGRPYEIFTGINDEDDGIMVPKNVTSGKIIKAYDNDGRKHYDFQF
                     QNRRGYKVTIEGLDGKFNPEFWNYAKLISGVLRYGMPIDQVIKLVSGLELDSETINTW
                     KNGVERALKRYLPNETEAKGQKCPVCGFESLVFEEGCLKCRNCGASKCG"
     gene            3373..3996
                     /locus_tag="PLI30_03180"
     CDS             3373..3996
                     /locus_tag="PLI30_03180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009128478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HOI78590.1"
                     /translation="MVIYYFCPMQDTYKTIEQAAEGYIIEKKSKFLSFVFHVESPEEV
                     KGIVDEHRKKYYDARHVCWAYMLGPEREEYRSNDDGEPSGTAGRPILGQINSYGLTNV
                     LIIVIRYFGGTLLGTSGLIKAYKEAAEDAVKNAEIVEKTVDRALTVRFEYVLLNDVMR
                     TLKQFEEVSWEQNFKESCTMKLCIRKSEFQRLHDSLSQIYGVKIEKE"
     gene            4082..6451
                     /gene="topA"
                     /locus_tag="PLI30_03185"
     CDS             4082..6451
                     /gene="topA"
                     /locus_tag="PLI30_03185"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018108870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HOI78591.1"
                     /translation="MAKNLVIVESPAKAKTIEKFLGSDFKVMSSYGHIRDLKKKDFSI
                     DIDNNFEPIYEIPADKKKVVTELKASAKNAETVWLASDEDREGEAISWHLYDVLDLKN
                     KDTKRIVFHEITKPAIQEAVKNPREINRNLVDAQQARRVLDRIVGFELSPVLWRKIKP
                     ALSAGRVQSVAVRLIVEREREIQNFKTEAAYRVVALFIKEENGQQYEIKAEYNKRLKT
                     KEEALALLEKLKTSVFSVGDVTTKPARKSPAPPFTTSTLQQEASRKLGFSVAQTMMVA
                     QRLYESGRITYMRTDSFNLSALAINTAKAEIIGQYGENYLKIRKYTTKSKGAQEAHEA
                     IRPTYINEHEAGGTAQEKRLYDLIWKRTIASQMADAEFERTVATIDISNAEGKFVATG
                     EVIKFDGFLRVYLEGTDEENGENEEGLLPPMKVKEVLPMQETIATERFTQRPPRYTEA
                     SLVRKMEELGIGRPSTYAPTISTIQNREYVEKKTVDGVERLYNILTLKNGKIKDADKK
                     ETAGTDKNKLVPTDIGTVVNDFLVEFFPKIVDYHFTANVEKDFDTIAEGKRQWNSAIK
                     DFYKVFHPIVEETMEMRMEHKAGERVLGQDPKTGRQVSVKIGRYGPLVQVGTPDEEEK
                     PLFASLQKTQSIETITLEEALRLFDLPRSLGEFREKEMTVGVGRFGPYIRHNNKFVSL
                     PKGVDPLKITAQEAVELIEAKEKKDREKIIKTFTEDPELQVLNGRYGPYIAYNKKNFK
                     IPKSQKPEELTFEQCKGIIDDGTQKTDSTKEAPQKKSKTAKTPAKKTKK"
     gene            6469..7050
                     /locus_tag="PLI30_03190"
     CDS             6469..7050
                     /locus_tag="PLI30_03190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008779105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOI78592.1"
                     /translation="MRIHPSQPADLPQLMQIYETARRFMQQTGNAGQWVDGYPKKELL
                     VEDIGREHSYVCLDEDNGIAGTFYFRVGEEPTYLNIFEGSWLNDDPYGVIHRIASSGK
                     HKGVAATCISWCLEKCGNIRIDTHRDNKVMQNILQKLGFSYCGIIYLENGAERLAYQY
                     TINPATPDKKINKIGSTFLKEARNTPPKMIQLL"
     gene            complement(6942..7499)
                     /locus_tag="PLI30_03195"
     CDS             complement(6942..7499)
                     /locus_tag="PLI30_03195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019539091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HOI78593.1"
                     /translation="MELLDVYVSLFAAFLKIGLFGFGGGYAMLPLIQQEVVDTHKWIS
                     VADFTDIVAISQTTPGPIAFNSATYIGYSAVTDMGFSTFHGIIGSAVCTLAVSIPSLV
                     IMTVVCAFFARLNNNPWMRASLSVLKPAVIGLIAAAALMLMNNYNFIDYKSWIIFGGV
                     FLASFKKVDPILLIFLSGVAGLIVY"
     gene            complement(7503..8063)
                     /locus_tag="PLI30_03200"
     CDS             complement(7503..8063)
                     /locus_tag="PLI30_03200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005678378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HOI78594.1"
                     /translation="MKPIFKQYWELFLVFFKIGAFTFGGGYAMVPLIRNEVVKKKNWL
                     DDEEFMDMLAIAQSMPGPIALNTSLFVGNKRMGFKGSLFSGLGIILPSFLVILLIAIF
                     FVQFKDNPVVERIFKGIRPAVVALIAAPLLGLGRSARISLKNVWIPVAVALSVSLAGV
                     SPVYIVLAAILLGILHFAHVKRKIKS"
     gene            complement(8453..9799)
                     /locus_tag="PLI30_03205"
     CDS             complement(8453..9799)
                     /locus_tag="PLI30_03205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005859947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrpB-like pyridoxal phosphate-dependent enzyme"
                     /protein_id="HOI78595.1"
                     /translation="MKTKKFLLPEREIPTHWYNVVADMQNKPQRMINPGTKEPLKPED
                     LFPLFAEELSRQEFNETDRWIEIPEEVRDKYKIYRPSPLVRAYSLEKALDTPAHIYFK
                     NESVSPVGSHKLNSALPQVYYNKIDGTTNLTTETGAGQWGTALAFAGKAFGLEIAVYM
                     VKISYEQKPYRRSLMQTWGAQVIASPSMSTKSGRKVLTERPTYQGSLGTAISEAIELA
                     MQTPNCKYALGSVLNHVSLHQTIIGLEAEKQMEMAGEYPDIVIGCFGGGSNFSGISFP
                     FLRHVIKGDKKTRFIAAEPASCPKLTRGTFKYDFGDEAGFTPLIPMYTLGHNFSPANI
                     HAGGLRYHGAGSIVSQLKKDNFIEAIDIPQLETFEAGVLFAQTEGIIPAPESTHAIAS
                     AIREAKMAKEEGIQKVILFNLSGHGLVDMSAYDQYLAGDLKNYEISTLEIEKYLQD"
     gene            10085..>10389
                     /locus_tag="PLI30_03210"
     CDS             10085..>10389
                     /locus_tag="PLI30_03210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005641117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HOI78596.1"
                     /translation="MTVATPKTKRNLIEVARQLFAKKGVDSTTMNEIAEASGYGRRTL
                     YTYFKNKKDVYKAVIGSELQKLYAALEDVIKRDLPADDKLMMFTFSRLSIIKEVVTR"
BASE COUNT         3102 a         2214 c         2510 g         2563 t
ORIGIN      
        1 tggggagaaa taagagctgc attgcatgaa tacgaagtgg aattatatct tccgaaattc
       61 aaaaccaaat acagcaagaa actgaatgaa gttcttacaa atatggggat gggattggct
      121 tttagcgatg ctgccgattt tagccggatg tcggcttttc cggcccggat tgcttttgta
      181 aaacaagata cctacatcag cacagacgag caaggaacgg aagctgctgc tgttacgacc
      241 gtaggtatgg aactaacctc catgcccgca attccgcaaa aagtagtttt caatgcagac
      301 aaacctttta tttatcttat ccaagaaaac tctaccggtg ccgttttgtt tatgggagcg
      361 gtgaaagata tttaataaac cggtatccga tttattcata tttcaataat tttttattca
      421 attgtcaaaa cactcagcga tttacagccg ctgggtgttt ttttagttta agtttggacg
      481 gaaagttgaa ctctgaaaaa ttattaatgt aaacgattga ttaacttatg agttgaacaa
      541 aatagaaaac ttttcttgct tcttgaaaac ttaaaatgct ttaaataaga taactaaaaa
      601 tttatttgga aaactttaca ctttgttgaa aactttttat tgattcgttt tgttaacttt
      661 gaaacatttc ttaaacttgt aatcccaaat aaccatactg tgggaaacgt tgtgttttgt
      721 tatttgggaa atagttgaat taatcggtca aaaaaaaatt aattgatgaa aaaagaaatt
      781 tatactttcg acgaggctta caacgcatcg ttggagtatt ttagaggcga tgaactggct
      841 gcaaaggttt gggtcagcaa gtacgctttg aaggacagtg aaggcaatat ctacgagaag
      901 acgcctgaag atatgcattg gaggctggca aacgaagtgg gaagaataga gaaaaaatac
      961 gccaatccgt tgagcgaaaa agaattgttc gatttgtttg accagtttaa atacatcatt
     1021 ccgcagggaa gcccgatgac ggggatcgga aacgattttc aggttgcatc gttgtccaac
     1081 tgttttgtga tcgggatggg tggcagcgcc gactcttacg gggcgatcat ccgtatcgat
     1141 gaagaacagg tgcagctgat gaagcgtcgt ggcggagtgg ggcacgacct gtcgcacatc
     1201 cgtcccaagg gatcgcccgt taagaattcc gcactcacgt ctacggggct ggttccgttt
     1261 atggaaaggt attccaattc cacgcgcgaa gtggcgcagg acggacgtcg gggcgcgctg
     1321 atgctgagcg tttccatcaa acatccggat gccgaggatt ttatcgacgc caaactggag
     1381 cagggaaaag ttaccggagc caatatttcg gtgaaaatcg atgatgattt catgaaggct
     1441 gcatccgaag gaaaaccgta cgttcaaaag tatcccattg attcggacac gccgaaatac
     1501 gaaaaaacga taaacgcgca ggagctttgg gaaaaaattg tgcataatgc atggcgttcg
     1561 gccgagccgg gagtgttgtt ctgggacacg attatccgtg aatcggttcc cgattgttat
     1621 gccgacctgg gtttccagac ggtttcaacc aatccttgcg gtgaaattcc gttgtgccct
     1681 tacgacagct gccgcctgct ggcaattaac ctctactctt acgtgatgaa cccgttcaag
     1741 gaaaacgctt atttcgattt tgagcttttc ggaaaacacg ttcgtctggc tcagcgcatc
     1801 atggacgata ttattgatct ggagtcggaa aagattgata agatcctcga caagatcaac
     1861 agcgatcccg aatcggaaga agtgaaatca tcggaacgta acttgtggga gaaaatccgc
     1921 cggaaaacac tcgaaggccg tcgcacaggg gtaggcataa ctgccgaagg cgatatgctc
     1981 gccgctttgg gggtccgcta cggttcggaa gaaggaaacg atttctcgga gagggtgcac
     2041 aagatggtgg cgctcaacgc ttatgcttcc tcggtggaga tggcgaaaga acgcggagca
     2101 tttgaaatat atgataccga aagggagaag aacaacccgt ttgtgaacag gcttaagaaa
     2161 gaagaccccc agctttacaa ggacatggtg aagcatggcc gtcgaaacat tgcctgcctg
     2221 accattgctc ctacggggac aaccagtttg atgtcgcaga ccacatcggg aatagaaccc
     2281 gtttttatgc cggtttacaa gcgtcgccgc aaggtcaatc cgagcgataa ggacgtgaaa
     2341 atcgattttg tggatgaaaa cggagattcg tgggaggaat acgtggtctt ccaccacaaa
     2401 tttgtgaact ggatggaagc aaacggttat cctaccgcaa aaaagtattc gaacgaagag
     2461 attgacgagc tggtggcaaa atcgccatac tacaaagcta cttccaatga cgtggactgg
     2521 ctcatgaagg tgcgtatgca ggggcgcgtg caaaaatggg tggaccacag catcagcgta
     2581 accatcaacc tacccggtga tgtgaccgaa gaactcgtgg gtgagcttta tatggaagcc
     2641 tggagaagcg ggtgtaaggg ttgtacggtg tatcgcgacg gatcacgctc gggtgtgctt
     2701 attacaaacg acaacaagga ggaagaaaag acaagtgcgg attgttacga actgccgcag
     2761 gtagtcactt cccgtcctac cgaactggat gccgatgtga ttaaattcca gaacaacaaa
     2821 gagaagtgga ttgcttttat cgggttgctc aacggaaggc cttacgagat ctttacgggt
     2881 attaacgatg aggacgatgg tatcatggtg cctaaaaacg ttacctccgg gaagatcatc
     2941 aaagcatacg ataacgacgg tcgcaaacat tacgacttcc agttccagaa caggaggggg
     3001 tacaaggtga ctattgaagg gttggacggg aagtttaatc cggagttctg gaactacgcg
     3061 aaactgattt cgggagtgtt gcgttacggc atgcctatcg atcaggtgat taagctggta
     3121 tcgggattgg aattggacag cgaaaccatc aacacgtgga agaacggcgt tgaacgggcg
     3181 ttgaagcgct atctacctaa cgaaaccgaa gcgaaaggcc agaaatgccc cgtatgcggc
     3241 ttcgaatcgc tggtctttga agagggatgc ctcaaatgcc gcaactgcgg agcttcgaag
     3301 tgcggataat ccggtatatg tccggcgttt tacaaaacca aaaaggcgat accaaccggt
     3361 atcgcttttt tgatggtaat ttactacttt tgtcctatgc aagacaccta taagactatc
     3421 gaacaagcgg ccgaaggtta tatcatcgaa aaaaaaagca aattcctttc gtttgttttt
     3481 cacgtggaat cgcccgagga agtaaaagga attgtggatg agcaccgcaa aaaatattac
     3541 gatgcacgac acgtttgttg ggcgtatatg ctggggccgg aaagggagga gtaccgttca
     3601 aacgacgatg gcgagccttc cggaacagca ggaaggccca tactgggaca aataaattcg
     3661 tacggactta ccaacgtctt aattattgtt atccggtatt tcggcggaac actcctgggt
     3721 acaagcggac tgatcaaagc ttataaagaa gcggcggaag atgccgttaa aaatgctgaa
     3781 attgttgaga agacggttga ccgggccctt accgttcgtt tcgaatatgt gttgcttaac
     3841 gacgtgatgc gtaccctgaa gcaatttgaa gaggtttctt gggaacagaa cttcaaagag
     3901 agctgtacga tgaagctctg catccggaag tcggaattcc aacggttaca cgatagcctt
     3961 tcacaaattt atggcgtaaa aattgaaaaa gagtgaaaaa ttctttttgc taatacaaaa
     4021 ttactacttt tgctaccgaa aatgaagtga aatatcccga cgggaatata aaacaagatg
     4081 aatggcaaag aatttggtta tcgttgagtc gcctgcaaag gcaaaaacta ttgagaaatt
     4141 cctgggcagc gattttaaag tgatgtcgag ttacggacac atccgtgatc tcaagaaaaa
     4201 agattttagc attgatatag acaataattt cgaacccatt tacgagatcc cggcagataa
     4261 gaaaaaggtt gtgacggagt tgaaagcttc cgctaaaaac gcagaaactg tctggctcgc
     4321 atctgatgaa gaccgtgaag gagaagccat atcctggcat ttatacgatg tgttggattt
     4381 gaaaaataaa gacactaaac ggattgtttt tcacgaaata accaagcctg ccatccagga
     4441 agccgtaaaa aatccccggg agataaaccg aaatttagtc gatgcccagc aggcacgacg
     4501 cgtattggac aggattgtcg ggtttgaact ttcacccgtt ttatggcgaa aaataaaacc
     4561 cgcactttct gcgggaaggg tccagtcggt ggctgtaagg cttattgttg aacgtgagcg
     4621 cgaaatccag aacttcaaga cagaggctgc ctaccgtgtt gtggccttgt ttatcaagga
     4681 agaaaacgga caacaatacg agataaaagc cgaatacaac aaacggttga aaacaaagga
     4741 ggaggcattg gccttgctgg aaaaactaaa aacttcggtt ttcagcgtgg gagatgttac
     4801 taccaagccg gccaggaaat cgcctgcgcc accgttcacc acatctaccc ttcaacagga
     4861 agcatcccga aaacttggtt tttccgtggc acagacgatg atggtggcac aacgcttata
     4921 cgaatcggga aggataacct atatgaggac ggactccttt aatttgtcgg cccttgccat
     4981 aaacaccgcc aaggccgaga tcatcgggca gtacggggag aattacctga aaatcagaaa
     5041 atatacaacg aaatccaaag gtgcacagga agcacacgaa gccatccggc cgacttacat
     5101 caacgagcat gaagcggggg ggactgccca ggaaaagcgt ttgtacgatt tgatctggaa
     5161 acgcaccatc gcttcgcaga tggcagatgc cgagtttgaa aggacggtgg ccaccataga
     5221 tatatccaat gccgaaggaa aatttgttgc cacgggagaa gtaatcaaat ttgatgggtt
     5281 cttgcgtgtt tacctggaag gtaccgacga ggaaaacggt gaaaatgaag aagggttgct
     5341 ccccccgatg aaggtgaaag aggtgttgcc tatgcaggaa acgattgcca cggaacgctt
     5401 tacccaacgt ccaccaaggt ataccgaagc ttcgctggta cgcaaaatgg aagaattggg
     5461 gataggccgt ccatcaacat acgcacccac catttcaacc attcagaatc gtgagtatgt
     5521 tgaaaagaaa accgtggacg gtgtagaaag gttgtacaac atccttaccc tgaaaaacgg
     5581 aaaaataaag gatgccgata aaaaggaaac ggcaggaacc gataaaaata aattggtgcc
     5641 caccgatata ggcacggtgg tgaatgactt cctggtggag ttttttccga aaattgtgga
     5701 ttaccatttc accgcgaacg ttgaaaaaga ttttgacaca attgctgaag gtaaacgtca
     5761 gtggaacagc gctataaaag atttctataa agtgtttcat ccgattgtgg aagagaccat
     5821 ggagatgagg atggagcaca aggccggcga acgggtgctg ggacaagacc ctaaaacggg
     5881 gcgtcaggta tcggttaaaa taggcaggta tggcccgttg gtgcaggtcg gtactcctga
     5941 tgaagaagaa aaaccgttgt tcgcttcttt gcaaaagacg cagtcgatag aaaccataac
     6001 gctggaggag gcactccggt tgtttgactt gccccgttcg ctcggtgagt ttcgtgaaaa
     6061 agagatgacc gttggtgtag gccgcttcgg accctatatc cgtcacaata ataaatttgt
     6121 ttcattgcct aaaggtgttg atcccttgaa gattaccgcc caagaagcgg ttgagctgat
     6181 tgaagccaag gagaaaaagg acagggagaa aatcattaaa acgtttacgg aagatccgga
     6241 attacaggtt ctaaacggta gatacggccc ctatattgct tacaacaaga aaaattttaa
     6301 aattccgaaa tcgcaaaaac ccgaagaatt gacatttgaa caatgcaaag ggattattga
     6361 cgacgggacg caaaaaaccg attctaccaa agaggctccc caaaaaaaat ctaaaacggc
     6421 taaaactccg gcaaaaaaaa cgaagaaata aacgggtcgc ttatgctcat aagaatccac
     6481 ccctcacaac cggccgattt gccacaacta atgcaaatct atgagaccgc acgccgtttt
     6541 atgcaacaaa ccggtaatgc aggacaatgg gtggacggtt atccgaaaaa agaacttctc
     6601 gttgaggata taggtcggga acacagttac gtttgtctgg acgaagataa cgggattgca
     6661 ggaacatttt attttagggt gggtgaggag cccacttatc ttaatatctt tgaaggatcg
     6721 tggctgaacg acgatcctta cggggttatt caccgcattg cctcttccgg aaaacacaag
     6781 ggtgttgcag caacctgcat ctcctggtgc ctggaaaaat gcggtaacat ccgtattgat
     6841 acacaccgag acaataaagt gatgcaaaac atactgcaaa agctcggatt ttcctattgc
     6901 ggaatcatct acctggagaa tggcgctgaa agactggcct atcagtatac gatcaatcct
     6961 gccacacccg ataagaagat caacaaaatc ggatctactt ttttaaagga agccaggaat
     7021 acccctccaa aaatgatcca actcttgtaa tcgatgaaat tgtaattgtt catcaacatc
     7081 agtgcagctg ccgcaatcaa tccgatgaca gccggtttca gaaccgacag ggaggcgcgc
     7141 atccaaggat tgttgtttag tctggcgaag aaggcgcaga caaccgtcat aataaccagc
     7201 gagggaatgc tgacagccag tgtgcataca gccgatccga taatgccgtg aaaagtgctg
     7261 aatcccatgt cggtaacagc ggaataaccg atgtatgtgg ccgaattaaa ggcaataggc
     7321 ccgggggtgg tttgagaaat agcgacaata tccgtaaaat cggctaccga tatccacttg
     7381 tgcgtatcca ctacttcctg ttgaataaga gggagcatag cataccctcc accgaaaccg
     7441 aaaaggccta tcttgaggaa agcggcaaac aacgatacat atacatccaa aagttccatc
     7501 cgtcagcttt tgatctttcg tttgacatga gcgaaatgca atatccccag caaaatagct
     7561 gccaagacaa tataaactgg tgaaaccccg gccagtgata ccgaaagagc taccgccacc
     7621 ggaatccata cgttcttcag cgatattctt gccgatcttc ccaggcccag caacggtgcg
     7681 gcaatcaatg ctactacagc cggccggata cccttgaaaa ttcgttccac aaccggatta
     7741 tccttgaact gtacaaagaa aatggcaatg agcagaataa ccagaaacga aggcaggata
     7801 attcccaatc ctgaaaacag acttccctta aatcccatcc ttttgtttcc tacaaacagg
     7861 gaagtgttca gtgcaatcgg cccgggcatg gattgagcaa tggcaagcat atccataaat
     7921 tcttcatcat cgagccagtt cttctttttg acaacttcat tccggattaa cggaaccatg
     7981 gcgtagccgc caccaaaggt gaaggctccg attttaaaaa aaaccagaaa caattcccag
     8041 tattgtttaa agataggttt catagttaat ttagcggctt accggccgga cagcaaagga
     8101 gataaaattg aaaaagcaga ctgcaaattg atgtaatcgc ttgctaatct gctttctata
     8161 agaagccgca tcggtggaat aagtcgaaac tccttatgac aggatttgag tgtcccggta
     8221 tctttgtaat ccgccgttcc gataaatcgg aatacccgaa ggcgcggccc gccattaata
     8281 cccaaaacga gtctcggttt ataaaatgta attgatgagt ttcccaatcg accaatgcgg
     8341 caataactca ttcatctttc taatgtcaaa gatacaaagc agaagtgaaa cagacaaata
     8401 tttttctgtt ttttttcacc tgaaatgcct gcttatgttg tataacagtt tcttaatcct
     8461 gtaaatattt ttcgatctca agggttgaga tctcgtaatt ttttaaatcg cctgccaggt
     8521 attgatcgta cgcgctcata tccaccagtc cgtgtcccga aagattgaag aggattactt
     8581 tttggatacc ttcttccttg gccatcttag cttcacgaat ggcagacgcg atggcatgag
     8641 tggattcggg agctgggata ataccctctg tctgggcaaa aagaacgccg gcttcgaatg
     8701 tttccagctg cgggatatct attgcttcga tgaagttgtc tttcttcagt tggcttacga
     8761 tgcttccagc accgtgataa cgcaatccgc ctgcgtggat gttggcggga gaaaagttat
     8821 gtccgagcgt gtacatcggg ataagggggg taaaccctgc ttcatcaccg aaatcgtatt
     8881 taaaagttcc gcgggtgagt ttcgggcacg aagccggttc tgcagcaata aaccgtgtct
     8941 ttttgtcccc ttttataacg tggcgcagga aagggaacga aatccccgag aaattggaac
     9001 ctcctccaaa acaaccgata acaatatcgg gatattcacc ggccatctcc atttgttttt
     9061 cggcttccaa gccaataatc gtctggtgca atgaaacgtg gttcaatacg ctgcctagcg
     9121 cgtatttgca gttgggcgtc tgcatcgcca gctcgatcgc ctctgaaatg gctgttccca
     9181 ggctcccctg ataagtgggg cgttctgtca atacttttct tcccgatttg gtggacatgc
     9241 tgggcgatgc aattacctgc gcaccccatg tctgcatcag cgaacgtcgg tagggtttct
     9301 gttcgtaact gatcttcacc atataaaccg caatctccaa tccgaaagcc tttccggcaa
     9361 atgccaaagc ggttccccat tgtccagcac cggtttcggt agtgaggttg gttgtaccgt
     9421 ctattttgtt gtaatatacc tgtggaagag ccgaatttaa tttatgcgac cccacggggc
     9481 tgacactttc atttttgaag taaatgtggg ccggagtatc cagcgctttt tccaggctgt
     9541 aagccctcac cagcggggaa ggacggtata ttttgtattt gtcgcgtact tcttcgggta
     9601 tttcaatcca ccggtcagtt tcattgaatt cctgacggga aagttcttct gcaaacaacg
     9661 gaaacaggtc ttccggtttc aacggttctt tcgttcccgg atttatcatc cgctgcggct
     9721 tgttttgcat gtctgccact acattgtacc aatgcgtggg aatctccctc tcgggtaata
     9781 aaaatttctt tgttttcatc agctgtattt ttttattttc gtttctttgg atgctaataa
     9841 aaaagcctga agctgagaac atcaggcagt aatgtcatct cgacctgtaa aagtcggttt
     9901 tgatgtacaa aagtataatt tattttttat aaagcaaata aaacgacagc tttaaaccgc
     9961 aaaaaagatg cctgctgcct cgtagcaaca aattttcccg attattccca ttttgttcgc
    10021 aaatcgtttc gtttcttaaa aactttgtgt tatttttgca caaacatatc ataattgtgc
    10081 atttatgact gtcgcaacac ccaaaacaaa acgaaacctc atcgaagtag cccggcaact
    10141 ttttgcaaaa aaaggcgttg acagcacgac aatgaacgag attgcagaag catcgggata
    10201 tggacgcaga acgctttata cctattttaa aaacaagaaa gatgtttata aagcggttat
    10261 cgggtcggaa ctccaaaagc tgtatgctgc cctggaggat gtgattaaac gtgacctgcc
    10321 agccgatgat aagttgatga tgtttacctt ttcccggctc agcataatca aagaggttgt
    10381 cacacgcaa
//
LOCUS       DAOSDR010000049        10302 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrimonas sp. isolate AMR_MDS_1826
            NODE_2471_length_10302_cov_3.750171, whole genome shotgun sequence.
ACCESSION   DAOSDR010000049 DAOSDR010000000
VERSION     DAOSDR010000049.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863355
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrimonas sp. (wastewater metagenome)
  ORGANISM  Petrimonas sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Dysgonomonadaceae; Petrimonas.
REFERENCE   1  (bases 1 to 10302)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10302)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:10:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,720
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 35
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10302
                     /organism="Petrimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2471_length_10302_cov_3.750171"
                     /isolate="AMR_MDS_1826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2023866"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..224)
                     /locus_tag="PLI30_03215"
     CDS             complement(<1..224)
                     /locus_tag="PLI30_03215"
                     /inference="COORDINATES: protein motif:HMM:NF023521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3575 domain-containing protein"
                     /protein_id="HOI78597.1"
                     /translation="MMYKLKTLSLIFFLTTGLFVSTHAQEISVKTNVPYWFTATPNVG
                     FEYVLGENLSLEFTAGFNPFKFGDTKQMKH"
     gene            723..3914
                     /locus_tag="PLI30_03220"
     CDS             723..3914
                     /locus_tag="PLI30_03220"
                     /inference="COORDINATES: protein motif:HMM:TIGR04056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOI78598.1"
                     /translation="MKRQISNLARGMVFILLWTISLCMFAQGITVRGSVVDANNEPLI
                     GVTIQVPGTTIGTVTDADGNFTLSNVPSNGQIEVSYVGMQTQLIPVNGRTTIQVTLSE
                     DTELLEEVVVVGYGTMKKSDLTGAVENVSAARLLDKPAFNVAQALSGKVAGLKIIERS
                     GAPGGIPMIRIRGTNSINSGNDPLFVVDDVVGVTNALTILNPNEIQSIDVLKDASATA
                     IYGARGANGVIIITTKRGVEGKPIVSYDGYVTQSYMQRRLNVLNNEQFFYVIRQAYMN
                     VSKYATNPNWSTLYDASILPPGKGSMTYSEMPWLFEKTTQGGYPIPMMGRDGNYYKPR
                     FENDWEGETFRPATSTNHQIAIRGGNKDVKLGTFLGYSNNNGLLLNSNFERFSGKLTG
                     DVKLTDWFNINSFLSLNRSKERTNDVSYFSGGMARSAIESYPMLPIKYPDDPEVYGIY
                     AGQYGTNADFPMGEVDCQSPVAISNGVETFRRRVQVNGALSLNFTITPNLTFKSNFAM
                     DLLEEKYYRYANRKISRSAQGAANLEAFKNYYWQNENYFNYNNTFGDHTIAGLLGASW
                     SRFTGENLTTWNDHFFDDFYGWHNIGIGTATRPAPSSSDRQNSLNSYFARINYDFMGK
                     YLFTATGRYDGSSKFGKNSKYGFFPSASVAWRMSEEEFMKNINGLTNLKFRASIGQTG
                     NQEIGSYVTQTFIGSGNVVLGNAGQPGLWPNSVGNPDLRWEKTTQYNFGVDLGFADNR
                     VTLMLDYYNKLTSDMLLDVPLPQSTTTGSVRLNYGKIRNEGFEAVLSTHNVKTRNFNW
                     YSDINFTRNVNTIEQLGPTGADILRNWWVGGANTILREGLPVAQFFGLNRLGTYGTQE
                     ASLAARYGMLPGDVKYEDRNNDGRISFVEDGIPMGSAFPIWDMNVNNSVTYKNIDFNL
                     DIRISYGAKKENRTNHSSEDRQGLANGKTSILDAWRPDHQNTMVAQVRPGNGGAYYQT
                     YPDTRWIEDASFVRGDGMTLGYTFPQDMTKKVGASRVRIYLNASNFFLLTKYSGYDPE
                     GSHNDNMDSITPGMDFFMYPRPSNYSFGVNLTF"
     gene            3936..5576
                     /locus_tag="PLI30_03225"
     CDS             3936..5576
                     /locus_tag="PLI30_03225"
                     /inference="COORDINATES: protein motif:HMM:NF019592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HOI78599.1"
                     /translation="MKTKSTNIKYVFITLMAGILFFSCNEFLAEHPTGTLTDEAQITS
                     IPGGVALATGAYRALPDWTGGGNVWGSSLPGSLEYPTGKAYAQYMGSDLWKYETDIMD
                     GTHPYFTTIWNNWYRGVRDANLAIKMLPGVTQMPEADRSKYMGEVRTLRAFYYFCLVR
                     LYGDVIANTTVLQNVNDAQQPRTSLVNIYDKIIVPDLEFAVNESNLQDTRSTDGRVTK
                     HVARAILADVYLTMAGYPYQEARTDTTKKWCETGSWAMTEYPVNTPSAKELLQKAKTQ
                     LDFLYGKYPIGNFSDLNNPEMDNQGGAIFQIQYASGIRDNGLIVHTLPLSLQISVFDV
                     ETGTFIPSTEYLNSYDPADIRMQERVYFYSSDTKAAKYDPNESPAPKFATRYLQKYYD
                     KDAIKRSARSGLNFNLYRYADILLMQTEVNWALSRLGVSIPDNDIIKGINEVRTRAGL
                     PSFSAEGLTLLDIMSERAYELVFENKMLWDQRRTRKALVDGSGQFTSIENFVGHQPVN
                     FNFEFSNKHLLSPVSGTELDNNRKCTQNFEWLPLQKGE"
     gene            5604..6782
                     /locus_tag="PLI30_03230"
     CDS             5604..6782
                     /locus_tag="PLI30_03230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI78600.1"
                     /translation="MKKYILTLFAVIFIISCGQDNVYEIPTDANGNIIWTGVSSTTTT
                     GISSIDNSFTVTATFATAKAGDVMKVELLQLQTPPEGGTTKQLLPLANTQKDVTVGSD
                     LKASVTYTRDQANLKGVGDYVMVMFNGATDYAKQRVDMTNAMTVSKPTVSGKEIEVAR
                     TAEVAYFNVNVTPKEGTFTGNVEVKRKNGTNGTWIDVTGSPFSGATQPYLVPIAGTDF
                     VIENDTMFYTFTATQGSYQEKIERTVIVRDPYFYLKKSATLTLGGSSAGRNLLINAAV
                     PANDPMALIAIEGSLMLKGGSAYLAAGKTIQFVPSTTAMYALNNSNTAIAAFESGTPA
                     NTLDPISGQGVYIFKAVTGPNPADVYYGMIMVTGVTPNQSVSFEYRIGNQYAHLTVIK
                     "
     gene            7004..8011
                     /locus_tag="PLI30_03235"
     CDS             7004..8011
                     /locus_tag="PLI30_03235"
                     /inference="COORDINATES: protein motif:HMM:NF012742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HOI78601.1"
                     /translation="MKKKKKVSIQDIADKLGVSKGTVSLVLSGKAEGSRISDEMSKKV
                     KETAEEMNYQPNEIARSLSTGKTMTIGVIVTDISNEFFGNLTFHIQEQAKKYGYTVIT
                     TNTNESLDEFSNAVTTLLNKQVDGIIFVPVDNGRQIAEKITRRHIPLVQVDRFYPNLE
                     ANYVIVDNYNVSAKATEHLIKKGLKKIAVVCYDFDVNALTERRRGCTDTLSKYKLLDS
                     NLIKNINYQDQEEEIKLAIHDLKNYPEKIDAVFFCSRKVFITGVRYMYKNAIKIPEDM
                     EVVCFDKIDSFAIANIPIIYIEQPIKKMGEKAVDVLMEQIKGSDTIKQCVFDTELMYM
                     N"
     gene            8027..>10302
                     /locus_tag="PLI30_03240"
     CDS             8027..>10302
                     /locus_tag="PLI30_03240"
                     /inference="COORDINATES: protein motif:HMM:NF013256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004559 - alpha-mannosidase activity
                     [Evidence IEA];
                     GO_process: GO:0006013 - mannose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 38 C-terminal
                     domain-containing protein"
                     /protein_id="HOI78602.1"
                     /translation="MNPIYKFFDKTYCRKVLFYFLLPFFVVEGLYSQGNQQEQVIFMN
                     TPVLFSENGQNYQQVIAGYTSENAGKIIFRYGGKNLLVSDTRKGFNKFLLTFPAVSKP
                     EEITLETTVNDGNAMQHKVVLIPPQKKWTIYLVQHSHTDIGYTRPQSEILAEQMRYID
                     YALDYCDQTDNLPDEARFRWTCESSWVAREYIRTRPESQIKRLLKRIREGRIEVTGMF
                     ANMSEIADENSLFDFLQPLREIKNSGIQVKTAMQNDVNGIAWCMPDYFKNTGVKYLIM
                     GINETRSIRPFDIPTCFWWEAPSGERLLAFRGEHYMTGNFYNIEKEDIDFTHMIWGIS
                     ELGKKGYPFDRIGIQFSGYFTDNAPPSTAACKFVEEWNSTHEFPKLKLAVASEFMEYV
                     EENHAEELPVYRNAWLDWWTDGFGSISRETAEIRKTQNAIQQDEGLLAMVTLLDGELS
                     PEIEKKIEHIKENIIFFDEHTVGAAESIQSPFSENSVKQWLQKGAYAWEAVKKETLMK
                     EEALARLQPYLHKANFPVIYIINPLGWDRSGSVELFIDSEVLPPEKKASITDVSTGKK
                     VPVQLVKKRAEGAYWMLEVKDIPALGYKALKISTTDELLSPEILPGTEIMENGFYRIE
                     VDKKTGAVKSLFDKELRLELVDRNNPYHLGQPIRETSEKRDTPPFHRAVSDHISVEKG
                     VQGTIWESIKIFSDMPGFSGAGEGATKGLELEIRLYRNTRKIEFKYSAHKEIITDPEA
                     LYIAFPFSLPEAKIVFETT"
BASE COUNT         3234 a         2176 c         2303 g         2589 t
ORIGIN      
        1 cagtgtttca tctgtttcgt gtcaccgaat ttgaacggat taaatccagc cgtgaactcc
       61 aggctgaggt tctcacccaa tacatattca aaccctacat tgggagttgc cgtaaaccag
      121 tacggaacat tggttttaac agaaatctct tgagcatggg tactgacaaa taatcctgtc
      181 gttaggaaaa aaatcaggga tagtgttttt aacttgtaca tcattttgct tcattttttg
      241 tgatatctat tattcaaaac aactttagtg ataaaaagtt tgggaataac gagattaccc
      301 taaataaatc cataaacacg cattttcaat actaatttac aataattatt gttaatgtat
      361 aattttatat aataatattt atgaatttaa ctcttgttta aaacaaccct tattttcaat
      421 atattcataa ataatattat atttttatat tatttattat gttttatttt gcatattata
      481 aataatattc tatttttgca atgaatattt agcattatta aaactcatgt gccgttagta
      541 tacacaagct gtaacagaac ctctgatgat catattcata atatatgata atttatgtgt
      601 cattttgtgc ttaatttttt ataatatatt acaattttac tttcattcta caaaaaatca
      661 atcactcaat accaagatgc gctcaggact ttaattaatt attaatctaa aaaaatgaaa
      721 gaatgaaaag acaaatttca aatctggcaa gaggaatggt gttcatttta ttatggacta
      781 tttccttatg tatgtttgct cagggcatta cagtaagagg tagtgtggtt gatgcaaaca
      841 atgaaccatt aattggggta acgattcagg ttcctggaac taccatcgga actgttaccg
      901 atgccgatgg gaattttacg ttaagtaatg ttccctccaa tggccaaata gaggtatcct
      961 atgtgggcat gcagacacag ttaattccgg tgaacggaag aacaaccata caggtgacct
     1021 taagtgaaga caccgaatta ctggaagaag tggtggtggt tgggtacggc accatgaaga
     1081 aaagcgacct gaccggtgcc gtcgagaatg tatctgcagc ccgcttgctg gacaagccgg
     1141 cattcaacgt ggctcaggct ctttcgggaa aggtagccgg attaaagatc attgaacgtt
     1201 cgggagcacc cggaggtatc cccatgatcc gtatccgtgg aacgaactcc atcaactcgg
     1261 gaaacgaccc gctttttgtg gtagatgacg tagtaggcgt caccaatgca ttaaccattt
     1321 taaatccaaa cgaaattcaa tccattgatg tattgaaaga cgcttctgcc acggctattt
     1381 atggtgcccg tggcgcaaac ggcgttatca ttattaccac caagcgcggt gtcgagggaa
     1441 aaccgatagt atcgtacgat ggttatgtca cacaaagtta tatgcaacgc aggttgaacg
     1501 tattgaataa cgaacagttc ttctacgtga tcaggcaagc ctatatgaat gtatccaaat
     1561 acgctaccaa tcctaactgg tcaacccttt acgatgcttc tatcctgcct ccggggaaag
     1621 gctccatgac ctattccgag atgccttggt tgtttgaaaa aacaacacag gggggatacc
     1681 caattccaat gatggggcgc gacggaaact attacaaacc gcgttttgag aacgactggg
     1741 agggcgaaac tttccggccg gcaacttcca ccaaccatca gatagccata cgcgggggga
     1801 ataaagacgt aaaactgggc actttcctag gatattcaaa caacaacgga ttgctgttaa
     1861 attcaaactt cgaacgtttt tcaggcaaac tcaccggaga tgtaaaactg accgactggt
     1921 tcaacatcaa ctctttctta tcgctgaaca gaagtaagga aaggaccaac gacgtttcat
     1981 atttcagcgg gggtatggca cgttccgcta tcgaatctta ccctatgcta cccatcaagt
     2041 atcccgatga cccggaagtc tacggaattt atgccggaca atacggaacc aatgccgact
     2101 tcccgatggg agaagtggac tgtcagtctc ccgtagctat cagtaacggg gtggaaacat
     2161 tcagacgccg cgtgcaggta aacggagctc ttagcctcaa tttcacgatt actccaaacc
     2221 tgacgtttaa aagcaatttt gctatggatc ttctggaaga gaaatattac cggtatgcca
     2281 acagaaaaat ttcccgttcg gcacaaggtg ctgcaaacct ggaagcattt aaaaattatt
     2341 actggcaaaa cgaaaactat ttcaactaca acaatacgtt cggagatcat acaattgccg
     2401 gtttgctggg agcatcctgg tcgagattta cgggtgagaa cctgaccact tggaacgacc
     2461 atttctttga tgacttttac ggctggcaca atatcgggat tggtacggca acgaggcctg
     2521 ctccaagcag cagcgatcgc caaaattcac tcaattctta ttttgcacgt ataaattacg
     2581 attttatggg caaatacctg tttacagcta caggacgtta tgacggctct tccaaattcg
     2641 ggaaaaactc caaatacgga ttctttccct ctgcctcagt ggcctggaga atgagtgaag
     2701 aagagtttat gaagaatatc aacgggctga ccaacctgaa attccgtgca agtatcggcc
     2761 agacaggtaa ccaggaaatt ggtagttatg taacacaaac ctttatcggc tcaggaaacg
     2821 tagtattggg aaatgcggga caacccggac tgtggcccaa ctcggtagga aatcccgacc
     2881 tgaggtggga aaaaacgaca caatacaact ttggagtcga tttggggttt gcagacaaca
     2941 gagtcactct gatgctggat tattacaaca agctgacatc agatatgtta ctggacgtac
     3001 ctttgccaca atctaccaca acggggagtg tgcggttgaa ctacggaaaa atacgcaacg
     3061 aaggatttga agccgtattg agcacccaca acgtgaaaac acggaatttc aactggtaca
     3121 gcgacatcaa cttcacccgt aacgtcaaca ccattgaaca attgggcccg acaggagcag
     3181 acatcttacg taactggtgg gtaggcggag ccaataccat tttaagggaa ggtttgccgg
     3241 tcgctcaatt tttcggatta aaccgtttag gaacctacgg aactcaggaa gctagcctgg
     3301 cagcccgtta cggcatgctt cccggcgacg ttaaatacga ggaccggaac aacgacggaa
     3361 gaatcagttt cgtagaagac ggtataccta tgggaagcgc tttccctatt tgggacatga
     3421 acgttaacaa cagcgtaaca tacaaaaata tcgacttcaa cctggacatc cgtatttctt
     3481 acggcgccaa gaaggaaaac agaaccaacc actcttcaga agaccgtcag ggcttagcca
     3541 acggaaaaac atccatcttg gatgcctggc gccccgacca tcagaatacg atggtggcac
     3601 aggttcgtcc cggaaacggc ggtgcatact accaaactta tcccgatacc cgttggattg
     3661 aagacgcttc ttttgtcagg ggtgatggga tgacgctggg ttacaccttc ccgcaggaca
     3721 tgacgaagaa agtcggagcc agcagggtga gaatatacct gaatgcttcc aatttcttct
     3781 tattgaccaa atattcaggc tacgaccctg aaggtagcca taacgataac atggatagta
     3841 ttacacccgg tatggacttc ttcatgtatc cgagaccgtc gaattactca ttcggtgtta
     3901 atcttacttt ctaacccaat taaactgcat tgaatatgaa aacaaaatca accaatataa
     3961 aatacgtatt cattacgttg atggccggga tattgttctt ttcctgtaat gaatttttgg
     4021 cagaacatcc caccggcaca ttgacagatg aagcacagat cacaagtatc cccggcggcg
     4081 ttgcactggc aacaggagcc tacagggcat tgcccgactg gacaggcgga ggtaacgtat
     4141 ggggaagcag cctcccggga agtttggaat atcccactgg aaaagcgtat gcacaataca
     4201 tgggatcaga cctttggaaa tacgaaaccg atattatgga tggaacgcat ccatatttta
     4261 caaccatatg gaacaactgg tacagaggcg tcagggatgc caacctagcc ataaaaatgt
     4321 taccgggcgt gacgcagatg ccggaagccg accggtcaaa atatatgggt gaagtccgca
     4381 cattacgcgc attctattat ttctgtctgg taagactgta tggcgatgtc atagccaata
     4441 cgactgtttt gcagaatgta aacgatgctc aacagccgcg tacgtcgctg gtaaatattt
     4501 atgacaagat cattgttccc gatctggaat tcgctgtaaa tgaaagtaat ctacaagata
     4561 cgcgatcaac agacgggcgg gttaccaaac acgttgccag agcaatactg gcggatgtct
     4621 acctgaccat ggccggttat ccctaccagg aagcaaggac tgacaccaca aagaaatggt
     4681 gtgaaaccgg gagctgggct atgaccgaat atccagtaaa taccccaagt gcaaaagagt
     4741 tgttgcagaa agccaaaacc cagctggatt ttctttacgg caagtatccg atcgggaatt
     4801 tcagtgattt gaacaatccg gaaatggaca atcagggagg agcaattttt cagatccagt
     4861 atgcatcggg catcagggat aacggactga tcgttcatac gttaccttta tcccttcaga
     4921 tttctgtatt tgatgtggaa accgggacat tcatccccag cacggaatac ctgaactcgt
     4981 acgacccggc agatatccgt atgcaggaaa gagtatattt ttacagcagc gatacaaaag
     5041 ctgcaaaata cgatcccaac gaaagccctg caccaaaatt tgccaccagg tacctgcaga
     5101 aatattacga taaggacgcc atcaagagga gtgctcgttc cggcttaaat ttcaacctct
     5161 accggtatgc cgatatttta ctgatgcaga cggaagtaaa ctgggccctg agcaggttgg
     5221 gagtatccat cccagacaat gatattataa aagggatcaa tgaagtgaga acaagagccg
     5281 ggttacccag tttttcagcg gaaggactta ccctgctcga catcatgagt gaacgcgctt
     5341 acgaactggt atttgaaaac aagatgttgt gggaccagag acgtacccgc aaagcactgg
     5401 ttgatggaag cggccagttt accagcattg aaaatttcgt tggtcaccag cctgtaaatt
     5461 tcaactttga gtttagcaat aaacatttac tgtctccggt ttcgggtacc gaattggata
     5521 acaacagaaa atgtactcag aatttcgaat ggctgccctt acagaaaggg gaatgatttt
     5581 attaatttga aaaatttaaa cgtatgaaaa aatatatttt aactcttttt gccgtcatat
     5641 tcatcatcag ttgtggtcag gacaatgtat atgaaatacc cactgacgca aatgggaaca
     5701 ttatctggac cggagtgtca agcaccacaa cgaccggaat aagctctatc gataactctt
     5761 tcacggttac cgccacattt gcaacagcta aagccggcga tgtgatgaaa gtggagttgc
     5821 tgcagttaca aacacctccc gaaggcggga caacaaaaca gttgcttccc ctggcaaaca
     5881 ctcaaaagga tgttacggtc ggaagcgacc tgaaggcatc tgtaacctat acccgggatc
     5941 aggcgaacct gaaaggggtg ggcgattatg tcatggttat gtttaacgga gctaccgatt
     6001 atgcaaaaca acgagtggac atgaccaacg ccatgacggt atcgaagccc acagtgtccg
     6061 gtaaggaaat tgaggttgcc cgtacggctg aagtagccta tttcaatgta aatgtaaccc
     6121 ccaaagaagg cacattcact ggaaatgttg aagtaaaaag aaaaaacggc accaacggga
     6181 cctggattga tgtaacagga tctcctttct cgggcgctac acagccttac ctagtgccta
     6241 ttgcgggaac cgactttgtt atcgaaaacg atacaatgtt ctacaccttt acagctacac
     6301 aaggcagtta tcaggaaaaa attgaaagaa cggttattgt aagagatccc tatttctacc
     6361 tgaaaaaatc ggccacactc acactgggcg gttcttcggc aggtagaaac ctgcttataa
     6421 acgcagctgt tcctgcaaac gaccccatgg ccctgattgc tattgaaggt tccctgatgc
     6481 ttaaaggtgg ttcagcatat ctggctgccg gcaaaaccat acagtttgtg ccttctacaa
     6541 ctgcaatgta cgcgctcaat aattccaata cggcaattgc tgcatttgaa agtggaacgc
     6601 cagccaacac attggatcct atttcaggac agggcgtata catattcaaa gcggtaaccg
     6661 gtccaaatcc agccgatgta tattacggaa tgattatggt taccggcgta actccaaacc
     6721 agtccgtttc gttcgaatac agaattggga accagtatgc tcaccttacc gtaataaagt
     6781 aggggaaagc ataaatacaa caacaccgcg aataagtccg cggtgttgtt gtataaaaaa
     6841 cggtcgtgaa gctatcggta tcatcggagc ctatttttag gcacaattgc aatgttaatt
     6901 aaatataaaa acaaagtatt ctggaagaat tacttcaata tttatttatc ttcgtaaaga
     6961 ttttttataa aacgttagtt aaccggttta gcaataaagg tacatgaaaa aaaagaaaaa
     7021 agtatccata caggatattg ctgataaact aggcgtttcg aaagggacgg tttcattggt
     7081 attgagcgga aaagcggaag ggagtcgtat aagcgacgag atgagtaaga aagtgaaaga
     7141 aacggctgaa gaaatgaact atcaacccaa tgaaatagcg cgaagcctca gtaccggcaa
     7201 aaccatgact ataggagtga ttgtcacgga tatttccaac gagttttttg ggaacctgac
     7261 tttccacata caagaacagg caaagaaata cggttacacg gtgattacga cgaacacaaa
     7321 tgaaagcctg gatgaattca gtaatgcggt gactacattg ctgaacaaac aggttgacgg
     7381 cattatattt gttcccgtgg ataatggacg gcagattgct gagaaaatta cgagaaggca
     7441 catccccctg gtacaggtag atcgttttta tcccaacctc gaagccaact acgtcatagt
     7501 tgataattac aacgtatcgg caaaagccac ggagcatctg ataaaaaaag ggttaaagaa
     7561 gattgccgtt gtttgttatg attttgatgt aaatgcctta accgaaaggc ggcggggatg
     7621 tacagatacg ctgagcaagt ataaattact ggattccaac ctgattaaaa acatcaatta
     7681 tcaggatcag gaggaagaga tcaaactggc tatccatgac ctaaagaatt atccggaaaa
     7741 aattgatgcc gtgtttttct gttcgcggaa ggtatttatt accggtgtga gatatatgta
     7801 taaaaatgcc attaaaattc ctgaagacat ggaagttgtc tgcttcgata agattgactc
     7861 tttcgctatc gccaatattc ccatcattta cattgaacaa cccataaaaa aaatggggga
     7921 aaaagcggtc gatgtgctta tggaacaaat caagggatca gacacaataa aacaatgtgt
     7981 gtttgatact gaactcatgt atatgaatta agaaactgat ctacttatga atccaattta
     8041 caaattcttc gataagacat attgcagaaa agtattgttc tattttcttt tgcccttctt
     8101 tgttgttgag ggactctact cacagggcaa tcagcaagag caggttattt tcatgaatac
     8161 tccggtcctc tttagcgaaa acgggcaaaa ttatcagcaa gttatcgcag gctatacttc
     8221 ggaaaacgca gggaaaatca ttttccgata cggcggtaaa aatttattag tttcggacac
     8281 gaggaaaggg tttaataagt tcttgctgac atttcctgcc gtaagcaagc ctgaagagat
     8341 tacccttgaa acaacggtta acgatggaaa tgcgatgcaa cacaaggttg ttcttatacc
     8401 tcctcagaag aaatggacca tctaccttgt ccagcattcc catacagata tcgggtatac
     8461 tcgcccgcaa tccgagatac ttgccgaaca gatgcgctac atcgattatg cgctggatta
     8521 ctgcgaccag accgataatt tgccggatga ggccaggttc aggtggacgt gtgaatcatc
     8581 gtgggtggcc cgtgaatata tccgtacaag gcctgaatca cagatcaagc ggttattgaa
     8641 acggatacgg gaagggagga tagaagtgac cggcatgttc gcgaatatgt cagaaatagc
     8701 cgatgaaaat tcgctgttcg atttcttaca gcctcttaga gagattaaaa attcaggaat
     8761 tcaggttaaa acagccatgc aaaacgatgt aaacggaata gcgtggtgca tgcccgatta
     8821 ttttaaaaat accggagtaa aataccttat catgggcata aacgaaacaa gatcaataag
     8881 gccgtttgat atacccactt gtttctggtg ggaagcgcca tccggggaaa ggttattggc
     8941 tttcaggggt gaacactaca tgaccggcaa tttttacaac atcgaaaaag aagacatcga
     9001 tttcacccat atgatctggg gtatttcaga gttgggaaaa aaaggctatc cgttcgaccg
     9061 gataggtatc cagttctcag gttattttac tgataatgcc ccaccttcca ctgccgcctg
     9121 caaatttgtt gaagaatgga actccaccca cgaatttcct aaactaaaac tagccgtagc
     9181 cagtgagttt atggaatatg ttgaggaaaa tcatgccgaa gagttgccgg tttacagaaa
     9241 tgcctggctc gattggtgga cggatggctt cggctctatt tcccgtgaaa cagcggaaat
     9301 caggaaaaca cagaacgcca tccagcaaga cgaagggtta ttggccatgg ttacactgct
     9361 ggatggcgaa ctatcgcctg aaattgaaaa aaaaatcgag cacatcaagg aaaatatcat
     9421 tttctttgat gaacatacgg ttggagcagc cgaaagtatt caatctccat tttccgaaaa
     9481 ttcggttaag caatggctgc aaaaaggagc ctacgcctgg gaggctgtaa aaaaagaaac
     9541 actgatgaag gaagaagctt tggccaggtt gcaaccctat ttgcacaaag caaattttcc
     9601 cgtcatttac attatcaatc ccttggggtg ggaccgctca ggatctgtag aacttttcat
     9661 cgattcagag gtattgcctc ccgaaaaaaa ggccagcata acggatgttt ctacaggaaa
     9721 gaaagtccct gtccagttgg ttaaaaaaag ggctgagggc gcttactgga tgcttgaagt
     9781 aaaagacatc ccagcactgg gttataaagc attaaaaatc agcacaacag acgagttatt
     9841 gtcaccagaa atacttcctg gaaccgaaat tatggagaac gggttttacc ggatcgaagt
     9901 ggacaaaaag accggtgcgg ttaaaagcct tttcgacaag gagcttcgtc tcgaattggt
     9961 tgaccgcaac aacccctacc atttgggaca acccatcagg gaaacatccg aaaaacgcga
    10021 caccccccca ttccaccggg ccgtgagcga ccacatcagt gtggaaaaag gtgtgcaggg
    10081 cactatttgg gaaagcataa aaatattctc agacatgccg ggattcagcg gcgctgggga
    10141 aggggcaaca aagggcttgg aacttgaaat aaggctttac cgcaataccc gaaaaataga
    10201 atttaaatac agtgcacata aggagataat caccgatccc gaagcattgt acatagcttt
    10261 tcccttttct ttaccggagg ctaaaattgt gttcgaaaca ac
//
LOCUS       DAOSDR010000050        10283 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrimonas sp. isolate AMR_MDS_1826
            NODE_2474_length_10283_cov_3.359112, whole genome shotgun sequence.
ACCESSION   DAOSDR010000050 DAOSDR010000000
VERSION     DAOSDR010000050.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863355
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrimonas sp. (wastewater metagenome)
  ORGANISM  Petrimonas sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Dysgonomonadaceae; Petrimonas.
REFERENCE   1  (bases 1 to 10283)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10283)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:10:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,720
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 35
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10283
                     /organism="Petrimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2474_length_10283_cov_3.359112"
                     /isolate="AMR_MDS_1826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2023866"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1018)
                     /locus_tag="PLI30_03245"
     CDS             complement(<1..1018)
                     /locus_tag="PLI30_03245"
                     /inference="COORDINATES: protein motif:HMM:NF019976.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nuclease-related domain-containing protein"
                     /protein_id="HOI78603.1"
                     /translation="MSKTYNTIGSLTALKSHLEENNIYDFKSLKEVIDFQSSFAISRQ
                     QLISHHKNLIEEEQSLLNVEMRNLETVIENQRQQTEQILNDEIDKLKQQLNSSISNNP
                     TNLFQKLTKELRHWNYKRKIKHKEKEFDSNVKATISHLTDDYQRKNSRFQFITSNFDD
                     AVKQSVHPSLSELERKKAIIDNLNSFIYGALGEQKVVKALEKLSDEYFLINDFSVSFS
                     PAIYNRQDNDYIKSVQIDHILVGPSGIFLIETKNWSEKSLENMSLRSPVQQIRRTSFV
                     LFKLLNNEMSNYHLRLDRHHWGDKKISIKNLIVMTNTKPQEEFQFVKILTLNELLGYI
                     NYFKP"
     gene            complement(1416..2177)
                     /locus_tag="PLI30_03250"
     CDS             complement(1416..2177)
                     /locus_tag="PLI30_03250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI78604.1"
                     /translation="MKNYLAILLFSIIASVSFGQTNYQDVVYLKNGSIIRGLIIEQVP
                     NASIKIETADRNVFVFQMGEIEKITKEAMEGKRADRFSDSDKRKGYIGLSLGVSVPVG
                     SFADNSDGMANAGTHLNLVNFGYLFSENFGIAGFWFGAANPLDVPGYDPWSYGGIMAG
                     PLLSFPQSQKVEWDFRPMIGYAATTLPDVGYGIEQDMSLALNLGALVRLNVGRKIALS
                     LSADYFYTKPEFVDYGFEQSIGTVSFGFGAAYRLK"
     gene            complement(2279..2497)
                     /locus_tag="PLI30_03255"
     CDS             complement(2279..2497)
                     /locus_tag="PLI30_03255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI78605.1"
                     /translation="MNHKRLRMHNPKSYRFHWVPGRFAPPYRFVTKDTDASPESTTAY
                     AVNRYTQGKENQPGVAIPAREPPGLVES"
     gene            complement(2487..3290)
                     /locus_tag="PLI30_03260"
     CDS             complement(2487..3290)
                     /locus_tag="PLI30_03260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI78606.1"
                     /translation="MKRFAILFFLGTVFLNAFSQDDDLKFILDSIVNEADLLYSYEKA
                     VWTASDILMSNSKLRKNFGGYVVSHSNDSIFATFIDKTQKQRIAKYYFTKADLNIPFH
                     SETGLSPLTETEQELVDIKIKIADQLSKKKYDVVFPHNYSPNLVLIKEKKEFRLYILM
                     GTSESDIIPFGNDYLFWANSKGKITNWKKFHSRMMSMPTKGPNGEKGTMAVHSHLKST
                     PYITATDICTFRLYGGLNDIDELMVICTATDKYYKYSIKTNSVVVTDEP"
     gene            complement(3451..3882)
                     /locus_tag="PLI30_03265"
     CDS             complement(3451..3882)
                     /locus_tag="PLI30_03265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI78607.1"
                     /translation="MKSKLLLTLMLIGTILLNYSCSATKKFAKTNPDVSTFIGSWVGE
                     SRTDGTLTTWLQKRFADGTYTILFIQINDNEVVRNIESGKWWIENGKFYEISSNVMEK
                     PDIFEYRIVSGDLISFKSTTVDYQFVDKRIEENYFDKIPLY"
     gene            complement(4252..4893)
                     /locus_tag="PLI30_03270"
     CDS             complement(4252..4893)
                     /locus_tag="PLI30_03270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI78608.1"
                     /translation="MKNSGGFKNKIMNDYKEYGKWTLHHTWVILVSYSISMVVLLIIH
                     GFFGFSMAEDGTYLSNAVMHIGSGFVLALGTGTLQRELLRKHIQMSFFWVVSLIIGFV
                     VAELVAGIVLWKLEIFRGLINIFNTDVHYPESLIFALAGLIAGIIQFRLLKPYYKKRI
                     FWILSSTIGWSLLILTTYLSPFAIPLGAFLYGAITGIVLYRILKSKTINENVT"
     gene            complement(5047..5268)
                     /locus_tag="PLI30_03275"
     CDS             complement(5047..5268)
                     /locus_tag="PLI30_03275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI78609.1"
                     /translation="MELLIFLLLLSSSAFAGTGSANDAILLYLVVIAVLSIIAAVLHS
                     VDFFRRIIRERRERKLNRHDEPAFGEHQG"
     gene            5486..5608
                     /locus_tag="PLI30_03280"
     CDS             5486..5608
                     /locus_tag="PLI30_03280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI78610.1"
                     /translation="MKAERNAMEGVVPNVPECSCPPISTLRAVAIPGKASFTIG"
     gene            complement(5960..9607)
                     /locus_tag="PLI30_03285"
     CDS             complement(5960..9607)
                     /locus_tag="PLI30_03285"
                     /inference="COORDINATES: protein motif:HMM:NF038128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor J domain-containing protein"
                     /protein_id="HOI78611.1"
                     /translation="MNTKDYLLKWIFVLLLFAAGTTGAHAQWIINESFEGGAIPAGWT
                     TYDANNDASTFRAFNKPTHAHTGDYICFVDSYGNNGNDWLVTPQVAIQAGDELNFFAR
                     SWYGTEDMEVKLSTTGNAISNFTVTLGSVTGLGTSYQEFNYDLSAYAGQNVYLAIHWM
                     QDTYGFIVDDIKVGQPQANDVGTLNVISPGSFIIIDTPVVPTATIKNYGTGEVTADFP
                     VVCNITDAEDNVVHTSEVTFTDPLAAGATAAVQFTGWTPTAEGSYSIEVYTAMAGDAD
                     LANDTVVSAFDVVQHYGTGGPDAMGYRWIDSGETGGPVYNWIDISATGTSTVTYGVNA
                     FAGDDNFSEAIPIGFDFPYYGINRTFFHADINGTLLLAANNWVKPYPDNGWDNDGNVF
                     NYHETIPGYSGMPALISVFWDDLIATEGTGNVYFQTFGTAPDRYTVVQWSNLAFVAGT
                     GGTPTLNFEVILHENGDIVMQYQNVANGQTGGANPHDFGLSATVGTQNDDCTTGLGYL
                     NEIVESSGYVGPDPAGNLLKNEMAIKFYPGEDNQVPMITTIKAWNTFNTYAHLQATIT
                     DLSGLASDSLYYNYGSGWMAVTHDSINQTNVYHYNLHDLPLGVTVEYYFAATDNSAAA
                     NRGVLETLNGEPLMFKVLPTNDVEVLILSPGNIPGFQDYQNIELPVYMNALENAGIAY
                     DVYNWAAFSQYTIPEQYNIVFAYSNSTGSTPVHDTLSKALIGFLDGGTQAMPRNIFFA
                     SDNLASAQHALPNSSYLRKFATAYLRTGFEVQPNPPIYGGGDGLGGPDSPGYHNGSII
                     GTAGSPIGTLNQEIPVYADSPDVIYPRTCPEVYAPEVSNPDIISRTAFRFEDGPISGN
                     AYSKGNPAAVWLDNIVYKSFFLSFDLSQFTATSDIQTTINEALEWFTPESFVITVEAM
                     PGDGGTVTGGGTYTSGSTATVTATAGDIYEFVNWTENGTIVSSEPAYSFTVTSDRALV
                     ANFTVVTYNVTAVVMPAEGGTVTGEGSYVYGAIASLTATPASDYVFLNWTSGGAVLST
                     EPEIEITVLSDTSLVANFESQIMQWEVTLYADPEVGGTVTGAGIYASGTEVTVTAEAA
                     TNFMFDFWEENGEVVSTEESYTFSINGDRVLTAHFKDITSTTEVDLKEFGISPNPTSD
                     YITIQNRTAGRQTIQSLELYNMSGNPVSVNYTAMGDSYNIDMKPFAPGLYILKITLKD
                     GTVRTCRVIRK"
     gene            complement(9746..>10283)
                     /locus_tag="PLI30_03290"
     CDS             complement(9746..>10283)
                     /locus_tag="PLI30_03290"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HOI78612.1"
                     /translation="YKFIVHKNIDLMKKERELGDAVRKLGEAKQELELLKSRQEVSEA
                     PVPEGASDDKTRPALSGDERKNLLLKLHEALVNDKLYTKADLTVEKLAKKLSTNRTYL
                     SQLINEEFQKGYSDLINEYRIREAMSLLSDPVSGQRYSIDAIARESGFNNISNFNMLF
                     RKHVGTTPSVFRKNAHSQ"
BASE COUNT         2614 a         2367 c         2273 g         3029 t
ORIGIN      
        1 ttggtttaaa ataattaata tatccaagaa gctcatttaa agttaaaatt ttcacgaact
       61 gaaactcttc ttggggtttg gtattcgtca ttacaatgag atttttgatt gatatttttt
      121 tgtcgcccca atgatgcctg tctaaacgta ggtgataatt actcatttca ttgttaagta
      181 atttaaacaa aacaaagctt gttctcctta tttgttgaac gggtgaacgt aaactcatat
      241 tttccaatga cttttcactc caattcttgg tctcaataag aaatattcct gatggcccca
      301 caagtatatg gtcaatttga acggacttta tgtaatcatt atcttgtctg ttatagattg
      361 ctggggaaaa cgaaaccgaa aagtcattta tcaaaaaata ttcgtcagat agcttttcta
      421 aagcctttac taccttttgt tctcctaaag caccataaat gaaactgttt agattgtcta
      481 taatagcttt ttttctttca agttctgata aagaaggatg aacactttgc ttaacagcat
      541 catcaaaatt tgaagttata aattggaaac gactattttt tctttggtaa tcgtcagtaa
      601 ggtggctaat tgttgctttt acgttactat caaattcttt ttctttgtgt ttgatttttc
      661 ttttgtagtt ccaatgtctt aattccttgg ttaatttttg aaataaattt gttggattat
      721 tgcttattga actattaagt tgttgcttca atttgtcaat ttcgtcattc aatatttgtt
      781 cagtctgctg tcgttgattt tctatcactg tttctaagtt tctcatttct acattgagca
      841 agctttgttc ttcttcaatt aggtttttat gatgagagat taattgctgt cgggaaattg
      901 caaatgagct ttgaaaatca ataacttcct ttaatgattt aaagtcgtaa atattatttt
      961 cttctaaatg tgattttaaa gccgtcagag aaccaattgt gttataagtc ttgctcattc
     1021 tgtctgccga ttgttaattg tatggtgtca ctacttattg cctgtaactc ttagatatcc
     1081 acacaaactt accacttttc tatactttcc aaaattattt tcaacaaaac ccaaaaaaag
     1141 cccgggattt ctcccgggac cggctgccgg attttgctgt tagtggagta ttatagatac
     1201 tccctgtttt ccccttgtgt tagaagcaaa aaattgttgg ttcaaaatac tgaagtgagg
     1261 gattaaacca tttcaattcc aggcaaaccc ggtcagtgat tctggccaaa acagtccggt
     1321 aattcgatgc aacaatggcc ggtatgtcgt atgcaaagaa agactgcctc ctccgctaat
     1381 taccggcgga gcctccggat cgaatgacgg ggtggttatt taagccggta ggcagctcca
     1441 aatccaaacg aaacggtgcc aatgctttgc tcaaaaccat aatctacaaa ttccggtttt
     1501 gtataaaaat aatctgcact taatgaaagg gcgattttcc tgcccacgtt cagcctgacc
     1561 aatgcaccta aatttaatgc aagcgacata tcctgctcta taccataacc tacatccgga
     1621 agagttgtcg cagcataccc tatcattggt ctgaagtccc attccacttt ttgcgattgg
     1681 gggaacgaca acaatggacc agccattatt ccgccataag accagggatc atatcccggc
     1741 acgtccaggg gattagctgc tccaaaccaa aaacctgcaa ttccaaaatt ttccgaaaac
     1801 agatagccga aattaaccag attcaggtgg gtgccggcgt tagccattcc atccgaatta
     1861 tcggcaaaac tgccaacggg caccgaaaca cctaaggata aaccgatgta tccctttctt
     1921 ttatcagaat cgctaaaacg gtcagccctt ttaccttcca tagcttcttt cgtaattttt
     1981 tcgatttccc ccatttgaaa aacaaaaaca tttctgtcgg ccgtctcgat cttgatggat
     2041 gcgtttggga cttgttcaat aattaaaccc cggattatac ttccgttttt caggtagaca
     2101 acgtcctgat aattagtttg tccgaacgac acagatgcga ttattgaaaa taaaagtatt
     2161 gctaaatagt ttttcatttt aacgcctttt agttttccta ctcttcaggt ttttcggttt
     2221 caccccgttt tttacaagtg ggcactggcc tccaccttat atataaaaac ttttattgtt
     2281 acgactcaac caatcccggc ggttctcttg ccggtatcgc tacgccgggt tgattttcct
     2341 tgccttgcgt ataacggttt acggcatatg ccgtagtgga ctccgggctt gcatctgtat
     2401 cttttgttac aaaccggtaa ggcggggcaa aacgcccggg aacccaatga aaccggtatg
     2461 atttaggatt gtgcatccgc aggcgtttat ggttcatccg ttactactac actatttgtt
     2521 tttatagagt acttgtaata cttatccgtc gcggtacata taaccatcaa ttcatctata
     2581 tcgtttaaac caccgtataa tctaaacgtg caaatatccg ttgcagtaat atatggggtt
     2641 gactttaaat gtgagtgaac agccatagtt cccttttcac catttggccc ttttgttggc
     2701 atagacatca ttcgggaatg aaatttcttc caattcgtaa tttttccttt tgaattcgcc
     2761 caaaaaagat aatcatttcc aaaaggaata atgtcggatt ccgatgtccc catcaaaatg
     2821 tagagtctaa attccttctt ttctttgatt aaaactaaat tgggactgta attatgcgga
     2881 aacactacat cgtatttttt tttcgacagt tgatcggcaa ttttgatttt aatatccacc
     2941 agctcttgtt ccgtttcagt caaaggcgat agtcctgttt cggaatgaaa cggtatattt
     3001 aaatcggctt ttgtaaaata atattttgca attctttgtt tttgagtctt gtcgatgaac
     3061 gttgcaaaga tggagtcgtt ggaatgcgaa accacatagc cgccaaagtt ttttctcaat
     3121 ttactattgc tcatcagtat atcgcttgca gtccagacag ctttttcgta gctatacagc
     3181 aaatccgctt cgtttactat tgaatcaagt ataaacttca aatcgtcatc ctgcgagaac
     3241 gcattcagga aaacggtacc taagaaaaat aaaattgcaa atcgtttcat agtatgtgtt
     3301 gtttctatga ttgagaacgt tgaaagtatg cagcgggcag gttctccgtg tccggcacct
     3361 tatcctctgt tgccgtaccc gccagcggac ccggagatgt cgaccagcac cgaaattctg
     3421 cccgatgtat tccctgtatt ggctgccgga ttagtataat ggtattttat caaagtagtt
     3481 ttcttctatt ctcttgtcca cgaattggta atcaacggtt gtgcttttaa aactaatcag
     3541 atctcctgaa acaatcctgt attcaaaaat atctggtttt tccataacat tggatgaaat
     3601 ctcatagaac ttaccatttt caatccacca ttttcccgat tcaatatttc ttacaacttc
     3661 attatcgtta atctgaatga ataaaatagt atacgttcca tctgcaaatc tcttctgtaa
     3721 ccatgtagtt aaggtaccat ccgtcctgct ttctccaacc caactcccaa taaaagttga
     3781 tacatcgggg tttgtttttg caaatttctt tgttgcggaa cagctgtaat tcagaagaat
     3841 ggttccaatc aacattaaag tcagaagtag ttttgatttc atacaagata atttttataa
     3901 gttagaatca atttaaagtt attgcaccgt tattttgcct tgcagtcaaa tggcagcata
     3961 ccacacaaac ttaccacttt tctatacttt ccaaaactat ttttaacaaa atcccaaaaa
     4021 aaacggattc ctccctgagc tggctgtcga atcgtgctgt cagtggagaa gtgactctcc
     4081 ctattttccc ctctcgttgg caaaaaccat ttattggttc agaattttga cttgaaggat
     4141 tagaccatct cagaaaacgg agaaccatac gtacggtggt ccggctgaaa agcgggataa
     4201 atcgtgagtg gcgcaccggt gggctttatc gcacaccggc cgtatactcg attaagtcac
     4261 gttttcattt atagtttttg atttcaatat tctgtataag actataccgg ttattgcacc
     4321 atatagaaaa gcgcctaatg gaatagcaaa gggactaaga tatgttgtta aaattagcaa
     4381 agaccagccg attgtgctcg acaatatcca aaatattctt tttttgtagt acggtttcaa
     4441 taatctgaac tgaataattc cagcaattaa tccagctaag gcaaaaatca aagattcagg
     4501 gtaatgaaca tctgtattaa aaatattaat cagtccccta aagatctcca gtttccataa
     4561 aacaattccg gctactagtt cggctactac aaacccgatt attaacgaca ctacccagaa
     4621 aaaagacatc tgaatatgtt ttcttaataa ttctctttgt aaagtaccag ttcccaaggc
     4681 caaaacaaaa ccgctcccta tgtgcatcac ggcattggat aaatatgttc cgtcttcagc
     4741 cattgagaat ccaaaaaagc catgaataat gagtaacaca accatgctga ttgaataact
     4801 gacaagaatt acccatgtat ggtgcaaagt ccatttccca tattccttat aatcattcat
     4861 gattttattt ttaaacccgc cactattctt catcccctta tcgtccgggc tccgctttaa
     4921 gcaaagggta catacgtcgc aaatcgggcc tcctattgtg gtggccgatg tttccaccgg
     4981 atggcctgaa catcttcggc ctgttgcctt ccgctttaat cttcaggaag tttttataaa
     5041 aaccgcttaa ccctggtgct caccgaacgc cggttcatca tgccggttca atttcctttc
     5101 tctccgctcc cggataattc ttctgaaaaa atcgacggaa tgcagcacgg cagcaataat
     5161 tgacaatact gcaataacga caagatacaa tagaattgca tcgttggcac tgcccgttcc
     5221 tgcgaaagca gaagaactta aaagcaataa gaaaatcagc aactccattt tcatggtttt
     5281 tccattttca ggttatttta tagaagctga actatatccg taaaggcacc ccgcatgcca
     5341 tcacgttatt cccaataatc ttttcgctta tgaaggccat tggtaaacac cctctcaaat
     5401 atacggctta ttccggtaaa tttcacactt ttgaatactc cggagcgatt attttctttt
     5461 gcttcatctt ggttttgagt aggatatgaa ggcggagagg aacgctatgg aaggggtggt
     5521 gccgaacgtg ccggaatgct cctgtccccc catttccacc ctcagagccg tggctattcc
     5581 cggcaaggca tcttttacaa tcgggtaatc cggatcctgg ccggcatgta tcctgttaag
     5641 cggcgcatat cccggttcag gatttctgtg cctctatagc ccgggaaata tgccaactct
     5701 agatttttgt ccattggggg atgggtcgcc tgcacgggcg attgtcacta cctgcgacca
     5761 atgaaccgga aataaaaagg acagcctgga tggaaacgct gaattctgaa ctctgaacgg
     5821 atcggatgag gtggaatgag aacccggcag cagggggcta atcggggttg attgggttga
     5881 tcgatccgta tgttactaaa aaccctcccg catccataaa ggatggggag ggctcagcgc
     5941 gcactccgta gtagtggttt tattttcgga tcactctgca ggtcctgacg gtaccgtctt
     6001 tcagggtgat ctttagtatg tagagtcccg gggcgaatgg tttcatgtcg atgttgtatg
     6061 aatcgcccat ggctgtgtag ttcaccgata cggggttgcc gctcatgttg tagagttcca
     6121 ggctttgtat ggtttgtctg ccggctgtcc tgttctggat ggtgatgtaa tcagaggtgg
     6181 gatttggact aatgccaaac tccttcaggt caacttccgt ggttgacgtt atatccttga
     6241 agtgggccgt gagcactctg tcgccgttta tactgaaggt gtagctctct tcggtggaga
     6301 ccacttcgcc gttctcttcc cagaagtcaa acataaagtt ggttgctgct tcggctgtta
     6361 cggttacttc cgttcctgag gcatatatgc cagctccggt aacggttcct cctacttcag
     6421 gatccgcata aagcgtgact tcccactgca taatctggct ctcaaagttt gccaccagag
     6481 aagtgtccga cagtactgtt atctctatct caggctctgt tgacagcact gcgcctccgg
     6541 aagtccagtt gaggaagacg taatcgcttg cgggtgtggc cgtcagcgag gcaatggcac
     6601 catacacata ggagccttct ccggtaaccg ttcctccttc tgccggcatt acgacagcgg
     6661 tcacgttgta ggttactacc gtaaagttgg ccaccagggc gcggtcggaa gtcacggtga
     6721 aggagtaggc cggctcactg gaaacaatcg tgccgttctc tgtccagttc acgaattcat
     6781 agatgtcgcc ggcagtagcc gttacggtgg cggtagagcc cgacgtatag gtgccgcctc
     6841 ccgttaccgt tccgccgtcg ccgggcatgg cttccactgt tataacaaag gattcagggg
     6901 taaaccattc cagtgcttcg ttgatggtgg tctgtatgtc gctggtggcg gtaaattgcg
     6961 acagatcgaa gctaaggaag aacgacttat aaacgatgtt gtccagccaa acggcagccg
     7021 gattcccttt ggagtaggca ttgcccgata tgggtccgtc ctcgaacctg aaggctgtgc
     7081 gggagatgat atccggattg gaaacctcgg gggcatatac ttccggacag gtgcgcgggt
     7141 agataacatc cgggctgtcg gcataaaccg gtatctcctg gttcagcgtg cctattggtg
     7201 aaccggctgt gccgatgatg cttccgttgt ggtaaccggg ggaatcgggt ccgcccaggc
     7261 cgtcgcctcc tccatagatc gggggattgg gctgtacttc aaatccggta cgcaggtaag
     7321 ccgtggcgaa cttcctcaga taactgctgt tgggcagagc atgctgggcc gatgccaggt
     7381 tgtcggaggc aaaaaagatg ttcctcggca tggcctgtgt gcctccatcc agaaaaccta
     7441 tcagggcttt cgacagcgta tcgtgaacag gggtggaacc cgttgagttg gaataggcaa
     7501 agacgatgtt gtactgctcc ggtatggtat actgactgaa ggctgcccag ttgtatacat
     7561 cgtaagcgat gcccgcattt tccagtgcat tcatgtatac gggcagttca atgttctggt
     7621 agtcctggaa accggggatg ttgccgggcg atagtatcag cacttccaca tcattggtgg
     7681 gcagcacctt gaacatcagg ggttcgccgt tcaacgtttc aagcacaccc ctgttggctg
     7741 ccgccgaatt gtcggttgcg gcaaagtaat attccacggt aacgcccaaa ggcaggtcgt
     7801 gcagattgta atgatacacg ttggtctggt taatggagtc gtgggtaacc gccatccagc
     7861 cgcttccgta gttgtagtag agcgagtcgg aagccagacc cgacaaatcg gtgatggtag
     7921 cctgcaggtg tgcgtatgta ttgaaggtat tccatgcctt tatggtggtg atcatcggca
     7981 cctggttgtc ttcaccggga tagaatttta tggccatctc gttcttcagc aggtttcctg
     8041 ccggatcggg gcctacgtag ccgctgcttt ccacaatctc attcaggtaa cccaggccgg
     8101 tggtgcagtc gtcgttctgc gtccccacgg tggcactcaa tccgaaatcg tggggattgg
     8161 ctccgccggt ttgtccgttg gccacattct ggtattgcat gacgatatct ccattttcgt
     8221 ggagtatcac ctcaaagtta agtgtgggcg ttccgccggt acctgccaca aaagccaggt
     8281 tgctccattg cacaaccgta tatctgtcgg gagctgtgcc aaaagtctgg aaataaacgt
     8341 ttccagttcc ttcggtggca ataaggtcat cccagaaaac ggagatcagc gccggcattc
     8401 cggaataacc gggaatcgtt tcatgataat taaaaacgtt gccgtcgtta tcccagccgt
     8461 tgtcggggta aggtttcacc caattgttag cggccagcag cagggtgccg ttgatgtcgg
     8521 catggaagaa agtgcggttt ataccataat aggggaaatc gaatccaatg ggaatggctt
     8581 ccgagaagtt gtcatcgccg gcgaaggcat ttaccccata cgttaccgtc gaggtgccgg
     8641 tagcactgat atctatccaa ttgtaaaccg gaccgccggt ttcgccgctg tcgatccagc
     8701 ggtaccccat tgcatccggg ccaccggttc cgtaatgctg cacgacatcg aacgccgaca
     8761 ccaccgtatc gttggctaag tcggcatcgc cagccatggc cgtatatacc tcaatactgt
     8821 agctgccctc cgcggttggc gtccatccgg tgaactgtac ggcagcggtt gctcctgcgg
     8881 ccagcggatc ggtaaaggta acctccgagg tatgcactac attgtcttcg gcatcggtaa
     8941 tgttgcagac tacggggaaa tctgcggtaa cttccccggt accgtagttc tttatggtgg
     9001 ctgtcggcac taccggagta tcaataatga tgaaactgcc cggactgata acgttcagtg
     9061 tgcccacgtc gttggcctgc ggctgaccca cctttatgtc gtcaactata aagccgtagg
     9121 tatcctgcat ccagtggatg gcaagatata cattctggcc ggcataggcg ctaaggtcgt
     9181 agttgaattc ctgatatgag gtacccaatc cggtaacgga gcccagtgta acggtaaagt
     9241 tggagatggc gtttccggta gtagagagtt taacctccat atcctccgta ccataccacg
     9301 agcgggcaaa gaagtttaac tcatcgcccg cctgtatggc cacctgtggc gttacaagcc
     9361 aatcgtttcc attgttgccg taactgtcaa caaaacagat ataatcgccc gtatgtgcat
     9421 gggttggctt attgaaggcc ctgaaggttg aggcgtcgtt gttggcatcg taggtggtcc
     9481 agcctgccgg aatggctcca ccttcaaaac tttcgtttat aatccattgt gcatgggcac
     9541 ccgtagtgcc ggcggcaaac agcagcagca caaaaatcca tttcaggagg taatccttag
     9601 tgttcataaa tgtcgttttt ttagttgtat tgaatcgcag cggcgtagta ctacgcccgg
     9661 acaacaaaat aaaggcacac tgccggaagg cacaactttt atagttgtga attggtaaaa
     9721 ttataactat aatcgggcag ataatttact gtgaatgagc gtttttacga aataccgagg
     9781 gggtagtgcc cacatgtttg cgaaacagca tattgaagtt ggagatgttg ttgaaacccg
     9841 attcgcgggc tatggcatcg atggaatacc gctggcccga caccgggtcc gacagcagcg
     9901 acatagcctc ccggattcgg tattcattga tcaggtcgga atatcctttc tgaaactctt
     9961 catttatcag ttgcgacagg taggtgcggt tggtggacag ctttttggca agcttctcca
    10021 ccgtaagatc ggctttcgtg tacagtttat cgttgaccag tgcttcgtgc agcttcagca
    10081 gcagattctt tctttcgtct ccactcagtg ccggtcgtgt tttatcgtcg gaagcgccct
    10141 ccggcaccgg cgcttcactc acctcctgtc tgctctttag cagctccagc tcctgttttg
    10201 cttcgccaag cttcctgacc gcatctccaa gctccctctc ctttttcatc aggtcgatgt
    10261 tcttgtgcac gataaactta tag
//
LOCUS       DAOSDR010000051        10221 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrimonas sp. isolate AMR_MDS_1826
            NODE_2494_length_10221_cov_4.317234, whole genome shotgun sequence.
ACCESSION   DAOSDR010000051 DAOSDR010000000
VERSION     DAOSDR010000051.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863355
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrimonas sp. (wastewater metagenome)
  ORGANISM  Petrimonas sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Dysgonomonadaceae; Petrimonas.
REFERENCE   1  (bases 1 to 10221)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10221)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:10:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,720
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 35
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10221
                     /organism="Petrimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2494_length_10221_cov_4.317234"
                     /isolate="AMR_MDS_1826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2023866"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            140..1477
                     /locus_tag="PLI30_03295"
     CDS             140..1477
                     /locus_tag="PLI30_03295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004293041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HOI78613.1"
                     /translation="MPAIRDFTQGGIFRQLITLAMPLMAVSFIQMTYNMVDIIWIGRL
                     GSKSVAAVGTVGMLMWMMNSFALLSKVSAEVSIGQSIGAKRLDKAMLYASHTTTIAII
                     SGLVFATFFFLFPQLVLSFFRLEADITQEAIRYLNIVTFSVPFMFLILNFSGIYIGTG
                     RSDIPFYFNAVGLVLNIVLDPLFIFGFGFIPEMGSQGAALATTLSQVVVFMLFIRHLR
                     RKKGVLGQFAFWIKLRKNYTLNILKLGVPVAAMNVYFSIINMNLTRIASIHGGHLGVT
                     SQTTGGQIEGVTWNTSQGFATALGSFVAQNFAAGKMDRARHAYRYTLRAMMALGLVVT
                     TSFMLFGSEIFSVFIPEKEAYLAGGDYLFIIGISQLFMMLELTTQGMFNGIGKTTPPA
                     IVSIVFNTLRVPLAVFLASAIGVNGVWWAISITSVVKGICSATWYYFSQRKYR"
     gene            1507..2220
                     /locus_tag="PLI30_03300"
     CDS             1507..2220
                     /locus_tag="PLI30_03300"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007667628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0016018 - cyclosporin A binding [Evidence
                     IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HOI78614.1"
                     /translation="MKSHATFVFLILVLALTMQACSSLKHSNEKEQKVLIQTTEGDIT
                     LKLYNETPLHRNNFIKLVNAKTYNGLLFHRVIKEFMIQGGDPQSKTAGPDTMLGDGDV
                     GYTVPSEFRTPQIYHKYGTIAAAREGDDSNPQKASSGCQFYIVVGKKFTNEQLDKLEE
                     SKIARYGNTNDSTYKFSTQARQDYINVGGTPHLDGNYTVFGEILKGMEVVEKISEAET
                     DKHDRPIKAIRIKKMKLIR"
     gene            2388..2651
                     /locus_tag="PLI30_03305"
     CDS             2388..2651
                     /locus_tag="PLI30_03305"
                     /inference="COORDINATES: protein motif:HMM:NF021169.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2024 family protein"
                     /protein_id="HOI78615.1"
                     /translation="MEAAVWDTYVTKKDGKFMHFDIIVPADLKDENVIHNYGREYLKA
                     KGQEGQPLTSKESKYCHTEKIKPQWIADIREKGYYIYEMENCD"
     gene            2717..3334
                     /locus_tag="PLI30_03310"
     CDS             2717..3334
                     /locus_tag="PLI30_03310"
                     /inference="COORDINATES: protein motif:HMM:NF024854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HOI78616.1"
                     /translation="MEDFQFYSDQTTEGKIVASLERISQAFRVLLWKKSKEFSLTPLQ
                     VQILIFLFTQNEEKKKVSYLAKEFNVTKATISDTVKTLEQKHLVVKDNEATDSRSFVI
                     NLTEKGNKIAGQTSSFAKEIYSPIAELSWGDKKNVLSSLIKLIIHLNKTGVISVLRMC
                     TTCSYYRPSDDEKKDHCTLLKRDLDHVHIRVDCREHKMLDFPTTV"
     gene            3346..3864
                     /locus_tag="PLI30_03315"
     CDS             3346..3864
                     /locus_tag="PLI30_03315"
                     /inference="COORDINATES: protein motif:HMM:NF012521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HOI78617.1"
                     /translation="MKKLIVMRHGKSSWDYAHLDDHDRPLNERGKKNTAKMGVYLLQK
                     TGKPELILSSSAERAYATAVIAAENMGYPKEQIVTDKELYLAWVEEILQCLSRVPNTV
                     SYCLIVGHNPGLTLLINHFGVALDNLPTASTACFEFDTQVWKDISPANVRFQWLKQAK
                     EIEMRDSGDTRE"
     gene            complement(3934..4911)
                     /locus_tag="PLI30_03320"
     CDS             complement(3934..4911)
                     /locus_tag="PLI30_03320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI78618.1"
                     /translation="MNYKLFIFFAAVALIVASCTQEPLVELQQIVPVLTVTAAAPNGN
                     NAPSSDTRLALTMGETAISTLWKEGDKIKPYFVQGTAIVEGTEITLTDEHLSDNKKNA
                     TFNIAVPDDINTANPYTLYCVHGSSSQIARGKILVDFSYTPFFPFSGGYGSVSPDTYY
                     TPITASAEIAFGVPTTSLTFNYLGAMQIICFKNAAGAEVAYTEMELVQVNPADAAGFY
                     AYKNGEGAPRYDLISKEVIYYGVSQGVISGSIWSDDVRKFSRFVLLTGNTMPATRLKV
                     KIDGMQKESINATSASAVPHQAGNAYCVYALWNGVDLQLTDKDFTSVRK"
     gene            complement(5049..5183)
                     /locus_tag="PLI30_03325"
     CDS             complement(5049..5183)
                     /locus_tag="PLI30_03325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI78619.1"
                     /translation="MRQENLQTNNAVEHYEAPDIEMIDIELNQNILGGSVPDMPVHDW
                     "
     gene            complement(5416..6507)
                     /locus_tag="PLI30_03330"
     CDS             complement(5416..6507)
                     /locus_tag="PLI30_03330"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HOI78620.1"
                     /translation="MAEIYYLLTWGTFWLMLLCFVFLLTVSVPRRDDLRSYKRVRLLL
                     AWGVFFLMTLVNLTELSMSDSGTNDDIWYTRLMTLWIGACVAPVFSIVNITLINSHFF
                     SWRNLVRDLVIPLVLVVGNVFLFEIFSAKSLAFLFAYVVFIVYYVFVVVAYTVIFLRN
                     YRQYSIRLNNYFSEPGSNLLKWTVTTQFLATSSGIMAFVSLFMPHGWLCVFALFLNIL
                     YPYYAIRFINYAFQFPVIAPVVEEVMEEEPVVRDSVFMLEDIVGEWEKDKRYLQPELN
                     IEMVAHELSTNRTYLSSYINTCKNKNFKEWISDLRIAEAQRLLLSEPKTPINEIGERV
                     GFSDKGNFSTRFSKSVGMSPSLWRKTHLK"
     gene            complement(6533..7741)
                     /locus_tag="PLI30_03335"
     CDS             complement(6533..7741)
                     /locus_tag="PLI30_03335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019539504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine dehydrogenase"
                     /protein_id="HOI78621.1"
                     /translation="MIYEPLRSTQFVVRELLLKADKQNISLVIGIPKENQKVEKRLAI
                     TPETTALLVESGYQVMIESDAGLAINYSDRYYSDSGANIVQTKAEVFQADIILKVSAP
                     TFDEVSMMKPRSSVFSFLQLYLISSETLELMAEKRINALAYELIYDDSQISPFVTAIS
                     EIEGAYSISVASELLSSAHGGKGILLGGVPGISPTEVVIIGAGVAGAVAARAALALGA
                     TVKIFDNDINKLRKIQHELGRMVFTSTLQPNVLRNVFRSADVVIGAMQYVNKTHLYRI
                     STDLIREMKKGSLIIDLRMSYGGCFETTMEACLPNHPQVFERFGILHFCELSISSRVA
                     RTASIALSNIFISMFSAMAESGGVGRFARFDRGFASGFYMFTGKMVNSYVANHFNWPV
                     NDIGLFLPGL"
     gene            complement(7738..8274)
                     /locus_tag="PLI30_03340"
     CDS             complement(7738..8274)
                     /locus_tag="PLI30_03340"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008146993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HOI78622.1"
                     /translation="MVRIYLIGYMGVGKTTVGKKLAKLLDIGFVDLDKFIESKYHKTV
                     PELFAERGEREFRLIEQKSLIEVSEIEDVVVSTGGGTPCFFENIRLMNQTGTTVYIHA
                     EPEELAARLRTSKTVRPIVSGKTGEDLTLFIAQHLKERERFYNQAQVIYKTDRLITKE
                     DIHLTVDRIADEIKKRLK"
     gene            complement(8291..9709)
                     /gene="cls"
                     /locus_tag="PLI30_03345"
     CDS             complement(8291..9709)
                     /gene="cls"
                     /locus_tag="PLI30_03345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019539502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HOI78623.1"
                     /translation="MNSVLMLILEILYALTIISVVIVVISENRNPIKTLSWVMVLVFL
                     PFVGLIWYLVFGQDFTKKQVITKRMYSKLKKRPLDEVGPLEEFAYPQEHASLIRLLKN
                     LDHTPLLGGNDVRFYTHAAEKFEDLLRDIENAKQHIHVEYYVFEGDNIGRKVRDALIR
                     KSLEGVEVRVIYDSFGSRKTPKAFFEEFRKAGIEAEPFLKVALPKITSRLNFRNHRKI
                     IVIDGQIGYVGGINIADRYIDGFKWGTWRDTHVRIEGKGVQGLQSIFLIDWFFVSQTL
                     ITSRKYFPELPVYGSISMQTVNSGPFREEREISHGILQAIYDAQKSIFIQTPYFIPPE
                     SMIEALQAAAIRGLDVRLIISKKSDVPLVQMASRSFVKEMLESGVKVYMYEKGFLHSK
                     LMVFDDSLTLIGSVNFDSRSFEHNFEVEAFIYDHDVAEKAMDIIVEDQRFSQVVSLRE
                     WMRRPMRLRFIESLMRLFAPLL"
     gene            complement(9745..>10221)
                     /gene="rsgA"
                     /locus_tag="PLI30_03350"
     CDS             complement(9745..>10221)
                     /gene="rsgA"
                     /locus_tag="PLI30_03350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004374529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HOI78624.1"
                     /translation="GYTCIKTSVLTGEGMNEVRELVCGKITLLAGHSGVGKSSIVNTL
                     QKDATQKVGKISDYHNKGMHTTTFSEMIELEKGGFVIDTPGIKGFGTIDMTTAEVSHY
                     FPEIFRISSKCKFYNCLHLNEPGCAVIKALENHYISQSRYQSYLNILEDANEEKYR"
BASE COUNT         3018 a         2309 c         2154 g         2740 t
ORIGIN      
        1 gaagtagctc agtcgataga gcattagcct tccaagctga gggtcgcggg tttgaatccc
       61 gtcttccgct ctaaaaagga gtagctgtaa aatagttact ccttttttta aggtaactgt
      121 gacaaccgat aaacaacata tgcctgcgat tcgcgatttc acacagggag gtattttccg
      181 tcaacttatc accctggcga tgccgttaat ggctgtcagc tttatccaga tgacctacaa
      241 tatggttgac attatctgga tcggccgctt gggcagcaaa tcggtcgcag ctgtgggaac
      301 agtgggtatg ctgatgtgga tgatgaactc cttcgcactc ctatccaaag tgagcgctga
      361 agtctccatc gggcagtcca ttggtgctaa aaggctggat aaggctatgc tctatgcttc
      421 gcacactact accatcgcca tcatttcggg cctggtcttt gccactttct ttttcctgtt
      481 tccccaactc gtcctttctt ttttcaggct ggaagcagac atcacgcagg aagccattcg
      541 ttacctgaac attgttactt tcagtgtgcc tttcatgttt cttatcctga atttctcggg
      601 gatttacatt ggaaccggaa gaagcgatat ccccttctat tttaatgcgg taggattggt
      661 tttaaatatc gtgctcgatc cccttttcat tttcggtttc ggatttattc ctgaaatggg
      721 atctcaaggt gctgcactgg ccaccactct ttcacaggta gtggtattta tgctttttat
      781 tcgccaccta cgaagaaaaa aaggtgtttt gggacagttc gccttctgga taaaactccg
      841 aaaaaactac acactcaata tcctgaaact gggagttccc gtagcggcca tgaacgtcta
      901 tttttctatc atcaacatga acctcacccg tatagcttcc attcacggag gacacctggg
      961 agttaccagc caaaccacag gtgggcaaat agagggagtt acctggaata cctcacaggg
     1021 gtttgcaaca gctctgggta gctttgtagc acagaacttt gctgccggta agatggacag
     1081 agcccgccat gcttatcggt atacattaag ggctatgatg gctttggggc ttgtagtgac
     1141 aacgtcgttc atgttgtttg gcagtgaaat tttttcggtt ttcattcccg aaaaagaggc
     1201 ctatcttgct ggtggcgatt atttatttat tattggtata tcccaattgt tcatgatgct
     1261 ggagctgacc acgcagggaa tgttcaacgg aattggaaag accactcctc cggccattgt
     1321 aagcatcgta ttcaacaccc tgcgcgttcc gctagccgtt ttcctggctt ctgccattgg
     1381 cgtcaacggt gtttggtggg ccatttcaat cacttctgtt gtgaaaggaa tatgctcggc
     1441 cacgtggtat tatttttcac agcgaaaata caggtaatta gtgataacaa gaaaaaacag
     1501 acaaatatga aatctcacgc aacatttgtt ttccttattt tagtcttggc gctgacaatg
     1561 caagcttgct cctcgttgaa acacagcaac gaaaaagaac agaaagtcct tatccagacc
     1621 actgaaggcg atattacgct aaaactatac aacgaaacac cgttgcatcg caataatttt
     1681 atcaagctgg tgaatgcaaa aacctataac ggattacttt tccatcgtgt aatcaaggaa
     1741 ttcatgattc agggaggaga tccccagtcc aaaacggcag ggccagatac aatgctgggc
     1801 gacggcgatg tcggctacac cgttccctcc gaattcagga ctccccaaat ttatcacaaa
     1861 tacggcacta ttgccgctgc gcgtgaagga gacgacagca acccgcaaaa agcatcatcg
     1921 ggttgccagt tttacattgt agtggggaaa aagtttacaa acgaacaact ggataagctg
     1981 gaggaaagca aaatagcccg ttacggaaat acaaacgact ctacttacaa attcagcaca
     2041 caagcgcgac aagattacat aaacgtcggc ggaacaccac acctcgatgg caattatacc
     2101 gtatttggcg aaatattgaa agggatggag gtcgtggaaa aaatatcgga agcagaaaca
     2161 gacaaacacg accgtcccat aaaggcaatc cgaataaaga agatgaagct gatacggtaa
     2221 cacatacaag ggctacgaaa cctatcaccg gctgaattaa tcgaaatgct atactttgat
     2281 aaagggctct atgaaaaaaa tcacctttcg tttgctttag ttagttatcc taactatatt
     2341 tgcggatggc aatggtgcca agacaaccgg cttaaaacgt aaaaatcatg gaagcagctg
     2401 tttgggatac ttatgtaaca aaaaaagacg gaaagtttat gcattttgac atcattgttc
     2461 cggcggattt aaaagatgaa aacgtaattc acaattacgg gagagaatac cttaaagcaa
     2521 aggggcagga aggacagcca ctcacttcca aagaaagcaa atattgccat acggaaaaaa
     2581 taaaacccca gtggatagcc gatatccggg aaaagggata ttatatttac gaaatggaga
     2641 attgtgatta accaccttat cctcggggaa taaaaaaaga tatcctatat aattgagatt
     2701 gacaaaatat aaatagatgg aggattttca gttttattcc gaccaaacta ccgaagggaa
     2761 gatcgttgct tccctggaaa ggatatctca ggcatttcgt gtactgctgt ggaagaaaag
     2821 caaggagttc tccctcactc cacttcaggt gcaaattttg attttcctgt tcacacaaaa
     2881 tgaagaaaaa aagaaagtga gttacctggc aaaagagttt aatgttacca aggcgacgat
     2941 aagcgatacc gtaaaaacac tggaacagaa acaccttgta gtcaaagaca atgaagctac
     3001 cgattcacga agctttgtta tcaacctcac ggaaaaggga aacaaaatag ccgggcagac
     3061 ctcgtctttc gccaaagaga tttatagccc tattgcagaa ttgagctggg gtgataaaaa
     3121 aaacgtattg tcaagtttga taaaattgat catccattta aacaaaacgg gagtaatttc
     3181 cgttcttcgt atgtgtacca cctgttccta ctatcgacct tctgatgatg aaaaaaaaga
     3241 ccattgtacg ttgttgaaac gggacttgga ccacgttcat ataagagttg attgtcggga
     3301 acacaaaatg ctggattttc caactacagt ataacaacag aagagatgaa aaagctgatt
     3361 gtaatgcggc acgggaagtc atcttgggac tatgctcacc tggatgatca cgaccgtcca
     3421 ttgaacgaga gaggcaagaa aaatacggct aaaatgggag tttacctgct tcagaaaacg
     3481 gggaaaccgg agctcatact ctcttcatct gccgaacgtg catatgcaac agccgttatt
     3541 gccgccgaaa acatgggata tccgaaagaa caaattgtga cggacaaaga attatatttg
     3601 gcctgggtag aggaaattct acaatgttta tcaagagtcc ccaacacagt cagctattgt
     3661 ctgattgttg ggcacaaccc cggattaacc ctcctgatca atcattttgg tgtagcgctt
     3721 gacaacttgc ctacggcatc gacagcctgt tttgagtttg atacgcaggt gtggaaagat
     3781 atttcaccgg caaatgtccg gtttcaatgg ttgaaacagg caaaagagat agagatgcgg
     3841 gacagcggtg atacccgcga ataacaggag gagaataaaa ccagcagttc tgcaaatata
     3901 gatcaatcag tgccggagtt attgctgctt tacctatttg cgtacagacg taaaatcctt
     3961 gtcggttagt tgcaaatcaa caccgttcca cagtgcataa acgcagtaag cgttaccggc
     4021 ttggtgcggg acagcagagg cgcttgttgc atttatactc tccttttgca taccatctat
     4081 tttcactttc agtctggtag ctggcatcgt gttaccggtc aacaacacaa agcgggaaaa
     4141 cttccgaaca tcgtcgctcc atatagaacc tgaaataact ccctgagaaa caccataata
     4201 aatcacttct ttagaaatca aatcatagcg gggtgctcct tcgccatttt tataagcata
     4261 gaagccggca gcgtcagcgg gattgacctg taccaactcc atttccgtat atgcaacttc
     4321 agctccagca gcgtttttga agcagattat ctgcatcgca cccaaataat taaacgtgag
     4381 cgaagtcgtg ggtacaccaa aagctatctc cgcagacgct gtaatgggcg tataataagt
     4441 atccggagaa acactgccat aaccaccgga aaaagggaaa aaaggagtat agctaaaatc
     4501 aaccagtatt tttcctctgg caatttggga agaggagccg tgcacacaat agagtgtata
     4561 ggggttagcc gtgttaatat catcggggac tgcgatgttg aaagtggcat tttttttgtt
     4621 gtccgacaaa tgctcatcgg taagggtgat ttccgttcct tccacaatgg ctgttccctg
     4681 cacaaaatag ggttttattt tgtcgccctc cttccacaat gttgaaatcg ccgtttcgcc
     4741 cattgtcagg gcaaggcgtg tatcggacga gggggcgtta ttgccgttgg gcgcggcagc
     4801 ggtcacagtc agtacaggca cgatctgctg caactccaca aggggttcct gggtgcagct
     4861 tgccacaatg agggcaacgg ctgcgaaaaa aataaataat ttataattca taatgtataa
     4921 cctgagaatt aatgttgttg catttataag accaaaagtc gcgagctaag actttcaaca
     4981 acagacttat ggattaatag gctgttttgt tttgggactt ccgaaaaata atcattgctt
     5041 acaatatctt accaatcgtg aacaggcata tctggaacac ttccgcctaa aatgttttga
     5101 ttcaattcaa tatcgatcat ttcgatatca ggagcttcat aatgttcaac cgcattgttc
     5161 gtttgcaaat tttcttgtct catatctaat tacagggtta aaataaaatt cttcggagaa
     5221 tttgcgacaa agctactggg agaaggcaaa agaagggttg tgtttccaac aaaaaattcg
     5281 tgtttgcaac aatcgtgcaa aaactgcctg ctttttaaca attggaagag acgggagacg
     5341 gaatgcggag gagaggagtt tacagcagat acctttgtca aagtccggaa ctttgacaaa
     5401 gctgcaacac atttttcatt tcaaatgcgt cttccgccac aaggaagggc tcatgccaac
     5461 acttttggaa aaacgggtac tgaagtttcc tttgtccgaa aatcccaccc gttcgcctat
     5521 ttcgtttatc ggtgttttgg gttcggacag caggaggcgt tgcgcctcgg caatacgcaa
     5581 gtcgctaatc cattctttaa agtttttgtt tttgcaggta ttaatgtaag aggaaagata
     5641 ggtgcggttg gtactcaatt catgggcaac catttcaata ttaagttcgg gctgaaggta
     5701 gcgtttatct ttttcccatt caccaacaat atcttccagc atgaaaaccg aatctctgac
     5761 gactggctct tcctccataa cttcttccac cactggggct ataacaggaa actgaaatgc
     5821 ataattaata aaccgtatag cataatacgg ataaaggata ttgaggaata aggcgaaaac
     5881 gcacaaccat ccatgcggca taaataaact tacaaaagcc attataccgc ttgaggtggc
     5941 cagaaactgc gtggttactg tccatttcag cagattggag ccgggttcgg aaaaataatt
     6001 gttgagccgg atagaatatt gtctgtagtt gcgaagaaaa atcaccgtat aggccaccac
     6061 cacaaacaca tagtagacaa tgaaaacaac ataagcaaaa aggaaggcca gtgatttggc
     6121 agagaaaatc tcgaacaaga atacgttgcc gacaaccagt acaaggggaa taaccaaatc
     6181 cctgacaaga ttcctccacg aaaagaaatg actgtttatc aaggtaatat ttacaatcga
     6241 aaaaacggga gccacacacg ctccaatcca cagcgtcatt agtcgcgtat accaaatgtc
     6301 gtcgttggtt ccggagtcgc tcatactgag ttcagttaga tttacgagcg tcatcagaaa
     6361 aaaaacgccc cacgccaaca aaagacgcac gcgtttatag cttcttaaat cgtcacgcct
     6421 aggaacggaa acggttaaaa gaaagacaaa gcataagagc atcaaccaga aagtgcccca
     6481 agtgagaaga taatatattt ctgccattat tcaacctcaa aagctcattt ctctacaatc
     6541 ccggcaggaa caacccgata tcgtttaccg gccaattaaa atgattggct acataagaat
     6601 tgaccatttt cccggtaaac atgtagaatc cggaggcaaa accgcggtcg aaacgggcga
     6661 accgtcctac tcccccactc tcggccattg ccgagaacat cgaaataaaa atattgctca
     6721 aagcgatgga tgctgtgcgt gctacgcggg agcttatgct taattcacag aaatgcaaaa
     6781 taccgaaccg ttcaaaaacc tggggatgat tgggtaaaca cgcttccatc gtagtttcga
     6841 aacaccctcc atatgacata cgcaagtcaa taatgagcga ccctttcttc atttcgcgga
     6901 tcaagtcggt ggagatacgg tacaagtgcg ttttatttac atattgcatg gctccgatca
     6961 ccacatcggc agagcgaaaa acattgcgca acacattcgg ttgcagggta gaggtaaaca
     7021 ccatccgacc caattcgtgc tggattttcc taagtttatt gatatcgtta tcaaatattt
     7081 tcactgtagc acctaatgca agcgcagcac gcgcagccac ggctcccgcg acacctgccc
     7141 ctataatcac cacttcggta ggcgaaatcc ctggaacccc acccaacaga ataccttttc
     7201 caccgtgtgc actgctgagc aattccgatg ctacagaaat ggagtatgct ccctcaattt
     7261 cactgatggc agttacaaaa ggagaaatct ggctatcatc ataaatcaac tcataagcca
     7321 gcgcatttat gcgtttttcc gccattagct ccagtgtttc cgatgagatc aggtacaact
     7381 gcaaaaacga aaacacactg cttcgtggtt tcatcatcga aacttcatca aaagtaggtg
     7441 ctgagacctt cagaataata tcggcctgaa aaacttcggc ctttgtttgt acgatatttg
     7501 ctcctgaatc ggaataatac cggtcggaat aattaatagc cagcccggca tcggattcaa
     7561 tcattacctg ataacccgat tcaacaagca gggccgttgt ttcgggagtg attgccagcc
     7621 gtttttccac tttttgattt tcctttggaa tacctattac aagcgagatg ttttgtttgt
     7681 ctgctttcag cagcaattca cgaacaacaa attgtgtgct tcttaagggt tcgtatatca
     7741 ttttagccgt ttttttattt cgtcagcaat tctgtcaacg gtcaggtgaa tatcttcttt
     7801 tgtgattaaa cggtcggttt tgtaaataac ttgtgcctga ttgtaaaacc gctccctttc
     7861 tttcaggtgt tgggcaatga ataacgtcag gtcctctccg gtttttccgg aaacaatggg
     7921 tcgaactgtt ttcgatgtcc gtaacctggc tgcaagttct tcgggttccg catgaatata
     7981 taccgtagtt cctgtttgat tcatcagccg gatattttca aaaaaacagg gggtcccgcc
     8041 gcctgtcgaa accaccacat cctctatctc tgaaacctca attaacgatt tttgctcgat
     8101 caggcgaaat tcacgttcac cccgttcagc aaaaagctct ggcacagtct tatggtattt
     8161 gctctcgata aacttatcta aatcgacaaa acctatatcg agcaacttag ccagtttctt
     8221 gccaactgtg gtctttccca cacccatata tccgattaaa tagatcctaa ccatattttt
     8281 ctataattgg ttataacaaa ggtgcaaaca aacgcattaa agattcaata aacctgagcc
     8341 tcatcggtct cctcatccac tcacgcaggg agacaacctg agaaaaccgt tgatcctcaa
     8401 caatgatatc catggctttt tctgccacat cgtgatcgta gataaacgct tccacctcga
     8461 aattgtgttc aaaactgcgt gaatcgaaat tcaccgaacc aatcaatgta agggaatcat
     8521 caaacaccat cagtttggaa tgcaaaaagc ctttctcgta catatacacc ttcaccccac
     8581 tttccagcat ctcctttacg aaagaacgtg atgccatttg caccaaaggc acatccgatt
     8641 ttttggagat gatcagcctt acatccagtc ctcggatagc agctgcctgc agcgcttcta
     8701 tcatggattc aggtggaatg aaatagggtg tttgaatgaa aatactcttc tgtgcatcgt
     8761 aaatggcctg aagaataccg tgagagattt ccctttcctc acggaatggc ccgctgttga
     8821 cagtctgcat ggatatgctt ccataaacag gaagctccgg aaaatatttc ctcgacgtga
     8881 taagtgtctg actcacaaaa aaccaatcga taagaaagat cgattgtaaa ccctgcacgc
     8941 ctttgccttc gatgcgtacg tgtgtatcgc gccaggttcc ccatttaaaa ccatcgatgt
     9001 acctgtcggc aatgtttatg cctcctacat atccaatctg tccgtcaatg acaattattt
     9061 ttcgatgatt ccggaaattt agccgcgagg taattttggg aagtgcaacc tttagaaacg
     9121 gttctgcttc gataccggcc tttctgaact cctcaaaaaa tgctttaggg gttttacgtg
     9181 aaccaaagct atcgtaaata acgcgtactt caacgccttc gagtgacttt cggatcaatg
     9241 cgtccctcac ttttctgccg atgttatccc cttcaaaaac ataatactct acgtggatat
     9301 gttgttttgc attttcaata tcgcgcagta aatcttcgaa tttttccgcg gcatgggtat
     9361 aaaaccggac atcgttccct cccagcaacg gggtatggtc aaggtttttc agtaaacgga
     9421 tcaaactcgc atgctcctgg ggataagcaa actcctccag cgggccaact tcatccagtg
     9481 gacgtttctt gagcttgctg tacatccgtt tggtaatgac ttgttttttg gtaaaatcct
     9541 ggccgaaaac aagataccag atcaatccca caaaaggcaa aaacacaagc accattaccc
     9601 atgaaagcgt cttaatggga ttcctattct cagaaattac cacaataacc acactgatga
     9661 tggttaaagc gtaaaggatc tcgagaataa gcatcaatac actgttcata actgtttctg
     9721 ttgtagtcct acggcacagc cagattaccg gtatttttct tcgtttgcat cttccaggat
     9781 attcaaataa gactgatacc ggctctgact gatgtaatga ttttccaacg cttttatcac
     9841 ggcacagccg ggttcgttca ggtgcaggca gttataaaac ttgcacttcg acgaaatcct
     9901 gaaaatctcg ggaaaatagt gagaaacctc agctgttgtc atgtcgatgg ttccgaatcc
     9961 tttgattccc ggagtgtcga taacgaagcc tcccttttca agttcaatca tttccgaaaa
    10021 agtagtagta tgcattcctt tgttgtggta atccgatatc tttcctactt tttgcgttgc
    10081 gtctttttgc agcgtgttta cgatacttga ttttcccacg ccggaatgtc cggcaagcag
    10141 tgtaatcttt ccacaaacaa gctcacgtac ttcattcatc ccctctcctg tgagcaccga
    10201 ggttttaatg catgtatacc c
//
LOCUS       DAOSDR010000052        10196 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrimonas sp. isolate AMR_MDS_1826
            NODE_2500_length_10196_cov_3.915294, whole genome shotgun sequence.
ACCESSION   DAOSDR010000052 DAOSDR010000000
VERSION     DAOSDR010000052.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863355
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrimonas sp. (wastewater metagenome)
  ORGANISM  Petrimonas sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Dysgonomonadaceae; Petrimonas.
REFERENCE   1  (bases 1 to 10196)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10196)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:10:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,720
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 35
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10196
                     /organism="Petrimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2500_length_10196_cov_3.915294"
                     /isolate="AMR_MDS_1826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2023866"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..252)
                     /locus_tag="PLI30_03355"
     CDS             complement(<1..252)
                     /locus_tag="PLI30_03355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI78625.1"
                     /translation="MKKLVALFSILATLMIVVSSCETKDPIVEKGTLQSATYNPATKS
                     FTLTYSSGQTETVNAVVDDKTDPPTATATLKDGTVIYAGD"
     gene            complement(326..934)
                     /locus_tag="PLI30_03360"
     CDS             complement(326..934)
                     /locus_tag="PLI30_03360"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006841655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HOI78626.1"
                     /translation="MLLKIYNENPNPREIEKVVSALRDGGIVVYPTDTLYGMGCDALN
                     VRAVEKICDLKGINPQKSNLSIICNDLSAISEYARVDTPTFKLMKRNLPGPFTFILPT
                     TSSLPKIYKNKKTVGIRIPDNPVIREIARMLGNPVLSTSVKDEGEEIEYTTDPDLIHE
                     KWSDIVDIVIDGGFGGIEPSTVVDCTSDEPVIVRQGKGVLNL"
     gene            complement(1012..1863)
                     /locus_tag="PLI30_03365"
     CDS             complement(1012..1863)
                     /locus_tag="PLI30_03365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019538251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase"
                     /protein_id="HOI78627.1"
                     /translation="MNHNLLKGKRGIIFGALNDQSIAWKVAERAVEEGATITLSNTPV
                     AVRMGDTNKLGEKLNAEILPADATNVEDLEMVFAKSVEILGGKIDFVLHSIGMSPNVR
                     KKRTYDDLDYDLLNKTLDISAISFHKMLQVARKLDAIQRGGSILALTYVAAQRVFYGY
                     NDMADAKALLESITRSFGYIYGRDKGVRVNTISQSPTMTTAGSGVKGMTDLMDFSERM
                     APIGNADADDCADYCVVMFSDLTRKVTMQNLYNDGGFSSMGMSLRAMKQYSNGLDENT
                     TLSDSES"
     gene            complement(2023..2706)
                     /locus_tag="PLI30_03370"
     CDS             complement(2023..2706)
                     /locus_tag="PLI30_03370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018357548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MIP family channel protein"
                     /protein_id="HOI78628.1"
                     /translation="MKKYLAEMIGTMVLVLMGCGAAVFAGAGQPFDPAGTLGVAFAFG
                     LSVVAMAYAIGSISGCHINPAITLGVLLTGRMSGKDAGLYIVFQIIGAILGSAILWFL
                     AKESGSTTTLTGANGFAEGQMAQAFVAETIFTFIFVLVVLGVTAKNGLNKFAGLAIGL
                     ALVLVHIVCIPITGTSVNPARSIGPAIFQGGAALAQLWVFIVAPFLGAVIAAFVWKGI
                     TVENEMETT"
     gene            2976..3701
                     /locus_tag="PLI30_03375"
     CDS             2976..3701
                     /locus_tag="PLI30_03375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006842623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOI78629.1"
                     /translation="MTVTYIFHSCYLLEFDGFSIVFDFYKDEKRDDGRFWISDYLLEK
                     PEDLYVFCTHSHPDHFNPEILKWGLNKTNVKYIFSKEVMDSREILVYQNIVFLDKLEE
                     YEDNRIKVKAFGSTDTGGSFVVNVSGKRFFHAGDLNNWHWNEEVPLLESTGYENSYLC
                     QLELLAENVDQLYLAMFPVDPRLGRDYMRGAEQLVNRISTDYFLPMHFGENYEKVNAF
                     SRYARLQNCTYLNVYKKGQSFEL"
     gene            3788..4156
                     /locus_tag="PLI30_03380"
     CDS             3788..4156
                     /locus_tag="PLI30_03380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005678529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HOI78630.1"
                     /translation="MKIKSRFDHYNINVFDLQRSIEFYDKALGLKEVRRKEASDGSFV
                     LVYLGDGETGFTLELTWLRDWDRPYNMGDNEQHLCVRVAGDYDRVRAYHKEMGCVCYE
                     NESMGLYFIVDPDGYWIEIL"
     gene            4204..6057
                     /locus_tag="PLI30_03385"
     CDS             4204..6057
                     /locus_tag="PLI30_03385"
                     /EC_number="1.17.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019538255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-en-1-yl diphosphate
                     synthase"
                     /protein_id="HOI78631.1"
                     /translation="MDYFNYHRRPTIDVHIGNITMGGNHPVVVQTMTNTNTLDTEGSV
                     AQCERIIAAGADLIRLTTQGVREAENLRLIHQQLREKGYSTPLSADIHFNPRAAHVAA
                     TVAEKVRINPGNFVDKQKTFAVVEYTDEEYVQELEKIRSKVVPFLQVCKEHGTAVRIG
                     VNHGSLSDRIMTRFGDTPEGMVESCMEYLRIALDEGFTDIVISMKASNTLLMTKAVRL
                     LVDRMDKENIHFPLHLGVTEAGDGEDGRMKSAVGIGALLSDGLGDTVRVSLSEDPEAE
                     VPVARKIVDYVAKREGHKPILGELYPGFSPFSTDKRETRAVRNIGGGFVPVVISDRNA
                     IADMSINPHFIPDYIYVGDNVPGNFPKGMKSIVDFPNWEDRIDNFPMFTAGNISDIKE
                     CQAAVKFLQLSYPQLTDEVLSVLKNTEKLVVILQTSHVNGVGEQRAFFHKLLNGHCDI
                     PVVLQRSYSEDVAEDIQVKGGIDFGTVLLDGFGNGIMISNTGKIDIAELDSYAFGILQ
                     AARVRTSKTEFISCPSCGRTLFDLRTTVALVKKHFSHLRHLKIGVMGCIVNGPGEMAD
                     ADYGYVGAEHGKISLYRKKELVEKNIPQEEAVEHLIQLIKDNGDWIEPDAN"
     gene            complement(6096..6530)
                     /locus_tag="PLI30_03390"
     CDS             complement(6096..6530)
                     /locus_tag="PLI30_03390"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014608.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HOI78632.1"
                     /translation="MEELTFRVKAHSENPTKTVVKARTFKMIIDEPTDLGGTNDGPNP
                     VEYVLAALSGCLNVMCHIVAQEMNIKLKGVEIKISGALNPEKLFGKETENRAGYKSIH
                     VEIKPDTDTDKDTLQKWIQTVEARCPVSDNLTNPTPVHIALA"
     gene            6738..6811
                     /locus_tag="PLI30_03395"
     tRNA            6738..6811
                     /locus_tag="PLI30_03395"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6772..6774,aa:Ile,seq:cat)
     gene            6933..7379
                     /locus_tag="PLI30_03400"
     CDS             6933..7379
                     /locus_tag="PLI30_03400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006843078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI78633.1"
                     /translation="MIIAVDFDGTIVEHAYPKIGRPIPFAIETLRMLHNDGHRLLLWT
                     VREGQLLQEAIDYCEQNGIYFFAANANYPEENREASPRKLKADLFIDDRNLGGLPDWG
                     IIYGAVQATVRGECSFEAFISNTPVPAKKEKKGFMGWLKLRNLFIF"
     gene            complement(7365..7886)
                     /locus_tag="PLI30_03405"
     CDS             complement(7365..7886)
                     /locus_tag="PLI30_03405"
                     /inference="COORDINATES: protein motif:HMM:NF019301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family membrane protein"
                     /protein_id="HOI78634.1"
                     /translation="MDTKYSSRQIITLTALRVVIGWHFLYEGMFKYIGDVWSSRSFLL
                     NSTGPLASLFKMWAQSDKAIHYIDMLNIWGLILIGLCLFLGFFERPAKLLGIILLSLY
                     YFTYPPFSSAAAGLYADGNYWIVDRNLIEIASLLVLASFPTGYRTGLDRFMANYPVRS
                     FFNKITGKPLKNK"
     gene            complement(7891..9435)
                     /locus_tag="PLI30_03410"
     CDS             complement(7891..9435)
                     /locus_tag="PLI30_03410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007654247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOI78635.1"
                     /translation="MKRRDFLMGISTVPILGAYSIGSTKNILKVLDDRRVDSKRLLDI
                     DQFDVMSERLPGSTGNEQTRIKIGLIGNGWRGESLLNSFGYFHPDYVDKMKTDGQYDR
                     WLPYQKRHENLYVDIAGICDTFDIHAERGIEVGRNNIREERSTPEISSGIKRYKTYQD
                     MIHSKDIDAVIVATPDHSHAQITIDAVKAGKHVYLEKPMCHSIEEAVELKKVVSASNI
                     VFQIGHENRQQMSYKLGRELYRKGVLGDVSMIQTFTNRNSLFGAWIREREFDHLGNQQ
                     TIDWKGFLCNAPWHEFDSKRYFNWQRYSDYGTGMIGNDFSHTYDCVNQVFEMGIPESV
                     MAMGGHFFYNVGIDDLPDVFNAIFYYPDRALTMTYDGTLKSGIYRQTKVLGSEATMEI
                     DRAIMLYKDPNSQRYKDIVEDSEEPFYYYAPNARVGVDGITSATATSYFKSGYGPTFI
                     DGKVIDATFLHLKEWIDAIRGQGKVSCGIDQGFEETVTFCLANLSYLHKKPIFWDKQN
                     EQYLIG"
     gene            complement(9460..>10196)
                     /locus_tag="PLI30_03415"
     CDS             complement(9460..>10196)
                     /locus_tag="PLI30_03415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI78636.1"
                     /translation="AALKIAQTYKKIQEPSGTWKLKLWIDGSPVTENDCIPIGIINFL
                     DRLESQYAVTEYVDTRKKAYDWIINNPLKTYNWSGQFEDVAPVEPYKNLSKDEASAFA
                     IYLFQNIHQKKEFRSVADDLLRFCEDQFIIWEKPMPQKQYNVEEWITPCVLEQYACYE
                     PINASVANMMEAYLTGYKATKNEVYKAKMIELANAVTVAQHANTGRYPTYWQLNERQK
                     KESGYIDWLNCTSYCVKVMLQISDSQ"
BASE COUNT         2759 a         2240 c         2258 g         2939 t
ORIGIN      
        1 atccccagca taaattacgg ttccgtcctt cagtgtcgca gtggctgttg gaggatcggt
       61 cttgtcatca accaccgcat ttaccgtttc ggtctgtccg ctgctgtaag tgagggtgaa
      121 tgacttggtg gcggggttat atgttgccga ttgcagcgtt cctttctcaa cgatcggatc
      181 tttggtctcg caagaggaaa ccacaatcat cagggtggcg aggatcgaaa agagtgcaac
      241 tagttttttc atagggttgg cttttttatt gtaacgaaca tttgactgcg aaagtaacga
      301 agattttaat cgggaagtcg atattttaaa gatttaacac tccttttccc tgacgcacga
      361 tcaccggctc atcggaagtg cagtccacta cggtagaggg ttcaatccca ccaaatccgc
      421 catcaataac gatatcgaca atgtcgctcc atttctcgtg gataaggtcg ggatcggtag
      481 tgtattcgat ctcctctcct tcgtccttta ccgaggtgct gaggacaggg tttccgagca
      541 tccgggcaat ttcgcggatt accggattat cgggtatacg gatccctact gttttcttgt
      601 ttttgtagat cttaggcagt gacgatgtgg tgggtaggat aaaggtgaaa ggtcccggca
      661 ggtttcgctt catcagcttg aaagtaggcg tatctacccg ggcgtattca ctgatggcgc
      721 tcaggtcgtt acagatgatg gagaggttgc ttttctgcgg attgatccct ttcaggtcac
      781 agattttttc tacggcacgc acgttcagcg catcgcaacc catcccgtaa agcgtatcgg
      841 tagggtagac cacgatgccg ccgtccctca gggccgaaac taccttctca atctctctcg
      901 gattgggatt ctcattgtat atcttcagta gcataacgaa cacaaaaata taaaaaaata
      961 gcttcgatac actgtccgaa gctattaaat tgtcatttat gggagcctgc ctcaactttc
     1021 actgtctgaa agcgtcgtgt tttcatcaag gccattgctg tactgtttca ttgccctgag
     1081 gctcatcccc atgctggaga atcctccgtc gttgtacagg ttttgcatgg ttaccttgcg
     1141 ggtgaggtcg gaaaacatca ctacacagta atcggcacaa tcgtccgcgt cggcatttcc
     1201 gatgggagcc attctttcgg agaaatccat caggtcggtc atccctttta ctccacttcc
     1261 ggcagtggtc atggtcggcg attgtgaaat ggtgttcacc cgaacccctt tgtcgcggcc
     1321 gtaaatatag ccaaaactcc gcgttatcga ttccagcaac gctttggcat ccgccatatc
     1381 gttgtagccg taaaatacgc gctgtgcggc aacgtaagtg agagcaagga tggaacctcc
     1441 ccgctgaata gcatccagct tgcgggctac ctgcaacatc ttatggaagg agatggctga
     1501 aatatccagt gtcttgttca gtaaatcgta atcgaggtcg tcatacgtgc gctttttacg
     1561 cacattgggc gacatgccta tggagtgaag tacaaaatca atttttccgc ccagtatctc
     1621 taccgattta gcaaaaacca tttccaggtc ttctacgttg gtagcatcgg caggaagaat
     1681 ttcggcattg agcttttcgc ccagtttgtt tgtgtctccc atccgcacgg caacaggagt
     1741 attcgacaat gtgatggttg ccccttcttc aacggctcgt tcggccactt tccaggcgat
     1801 ggactgatcg ttcaacgccc caaaaataat gcctcttttg ccttttaata aattgtgatt
     1861 cattttgact ttttgtttat gaaataaaaa actgcacaaa agtacaaata atgtgcaaac
     1921 ttataaacat ctaaacaaat aaagatcaaa tatgttcttt ccctcgcctt attttgtgct
     1981 ttttactcaa aaataaggtt tctgcaaggt gaatcatgcg attcaggttg tttccatttc
     2041 gttttcaacg gtaattcctt tccagacgaa ggctgcgata actgcaccca gaaaaggcgc
     2101 tacgatgaac acccaaagtt gagcaagtgc agcacctcct tggaagatag ccggtccgat
     2161 gcttcgcgct ggattaacgg aagtccccgt gatgggaata caaacgatgt gtaccagcac
     2221 caatgcaagg cctatcgcca ggccggcaaa cttgttcagt ccgttttttg ctgtaactcc
     2281 aagcacaacc agaacaaaaa taaaggtaaa tatcgtttct gcaacgaaag cctgagccat
     2341 ttgtccttct gcaaaaccgt tagcacccgt aagcgtagtg gttgagcccg attcttttgc
     2401 caaaaaccaa agaatagcag agcctaaaat ggcgcctata atttggaata caatgtacaa
     2461 tcccgcatct ttcccgctca tccggccggt caacaaaacg cccaacgtga tagcggggtt
     2521 aatgtggcat cccgaaatac tgccgatagc gtaagccatg gccactacag agagtccgaa
     2581 cgcaaaggct acgcccaatg tccctgccgg gtcgaaaggc tggccagcac ctgcaaacac
     2641 ggctgctccg cagcccatca acaccagtac catcgtaccg atcatttctg ctaaatactt
     2701 tttcataact ttttgaatga atttttgttg aaattaaaca taggtaaaac caaaattaag
     2761 cagaattaag gtttatgcag aagaatactt gagctaataa aacaaacggt gataaattcc
     2821 ccatttacgt ttttttgccc attttttacc ttttcatcat tcactaggca ctcattcaca
     2881 aaggtatgct ttttacagtt tttatcaaat tttatagccc ttaagatgaa caaaattccg
     2941 tagaattgtt ataaagataa gttaagatgt aaattatgac agtcacctat atttttcaca
     3001 gttgttatct gcttgaattt gatggttttt caatcgtttt cgatttttac aaagacgaaa
     3061 aaagagacga tggacgtttt tggataagtg attacctgct tgaaaagccg gaggacctat
     3121 acgttttctg tacccattca catcccgatc acttcaatcc ggaaatattg aaatgggggt
     3181 taaacaagac aaacgtgaag tatatttttt caaaagaggt gatggatagc cgggagatat
     3241 tggtttatca aaacatcgtt tttctcgata aactagagga gtacgaagac aaccggataa
     3301 aagtaaaagc attcggttcc acagataccg gaggatcgtt tgtggtcaat gtctcgggga
     3361 agcgcttttt tcatgccgga gatctcaata attggcactg gaatgaagaa gttcccctgc
     3421 ttgagtccac gggttatgaa aatagttatc tttgtcagct ggaactgttg gcggaaaatg
     3481 tagatcagct ttatctggcg atgtttcccg ttgatccccg gttgggaagg gactacatgc
     3541 gtggagcgga acagttggtc aacaggatat ctactgatta ttttctgccc atgcattttg
     3601 gagaaaatta cgaaaaagtc aatgcattca gccggtacgc ccgattgcag aactgtacct
     3661 atctcaacgt gtataaaaaa ggacagtcgt ttgaattata aaaaggagtc attagcgctt
     3721 acaacaggcg cggggtttcc ggtatattgg ttccgggttc caacagaata aacagtttaa
     3781 aaacaatatg aaaattaaaa gcagatttga tcattacaat atcaatgtgt ttgatcttca
     3841 gagaagtatc gagttttatg ataaggctct gggtttgaaa gaggtgagaa gaaaagaagc
     3901 ttctgacgga tcgtttgtcc tggtttatct gggcgacggt gaaaccggtt ttacccttga
     3961 actgacctgg ctacgggatt gggacaggcc ttacaacatg ggcgacaatg agcagcacct
     4021 gtgtgtacgg gtagcgggag attacgatcg ggtaagagcc tatcacaaag aaatgggctg
     4081 tgtgtgttat gaaaatgaat ccatgggatt gtattttatc gttgatccgg acggatactg
     4141 gattgaaata ctctaaacat ttgcgcagtt cgggcgttta tttctcacgt aaaacaattt
     4201 ataatggatt atttcaatta tcaccgccgt ccaaccatcg atgtacacat cggaaacatc
     4261 accatggggg gaaatcatcc ggttgttgtg caaacaatga ccaataccaa cacactcgac
     4321 accgaaggga gtgttgccca atgcgagaga attatcgcag cgggggccga cctgatccgg
     4381 ttaaccacgc aaggtgttcg tgaagcggaa aacttaagac ttattcatca gcaattaagg
     4441 gaaaaaggat acagtacgcc gctttcggct gacattcatt ttaatccgcg tgctgcgcac
     4501 gtggcagcta ccgttgccga aaaagtacgt atcaatcccg ggaacttcgt tgacaagcaa
     4561 aaaacgtttg ccgtagtgga atataccgat gaggagtatg tacaggaatt ggaaaagatc
     4621 cgttccaagg tagttccttt cctacaggtt tgtaaagaac acggtacagc ggtgcggatc
     4681 ggtgtcaatc acggatcact atcggaccgg atcatgaccc gttttggtga caccccagaa
     4741 gggatggtag aatcctgcat ggagtacctg cgtatcgctc tggatgaagg ttttaccgat
     4801 attgttatat cgatgaaagc gtctaacaca ttgctaatga ccaaagccgt tcgtttgttg
     4861 gtagacagaa tggataagga aaatatccat tttccgttgc atctgggtgt gaccgaagca
     4921 ggtgatggtg aagacgggag aatgaaatcg gctgtaggta tcggagcgtt gctctccgac
     4981 ggcttgggcg ataccgtccg ggtgtcgctg agcgaagatc ccgaagcgga agtccccgta
     5041 gcccgaaaaa tagtagacta tgtggctaag agagaaggac ataagcccat attgggtgaa
     5101 ctctatcccg gattctctcc cttctctacg gacaaacgag aaacacgtgc cgtgagaaat
     5161 atcggtggag gatttgttcc ggtggttatc tccgacagaa atgcaatagc cgacatgtcg
     5221 atcaatccgc atttcattcc agattacata tacgtgggtg acaacgttcc cggtaatttt
     5281 cccaaaggga tgaagtcaat agtagatttc ccaaactggg aagacaggat cgataacttc
     5341 ccgatgttta ctgccggaaa tatttcggat atcaaggagt gccaagcggc tgtcaagttt
     5401 ctacaacttt cctatccaca actgacagac gaagttttat ccgtgctgaa aaacacggaa
     5461 aagttggtcg ttatactcca gaccagccac gtgaacggag tgggagagca gcgagcattt
     5521 ttccataaat tactgaacgg acactgtgat attccggtag tgctgcagcg cagttattcg
     5581 gaagacgtgg cagaggatat ccaggttaaa ggggggattg attttggaac tgttctgctg
     5641 gatggtttcg gtaacgggat catgatatcc aacacgggga aaatcgatat tgccgaactc
     5701 gactcctatg cctttggcat tcttcaggct gcccgtgtac gcaccagcaa aacggagttt
     5761 atctcttgtc ccagctgcgg acgcacgctt ttcgacttgc ggacgactgt agcgctggta
     5821 aagaaacatt tctctcacct gagacacctg aagatcgggg tgatgggttg cattgtgaat
     5881 ggtccgggtg agatggccga tgcagattat ggttacgtgg gggcagaaca cggtaagata
     5941 tcgctctata ggaagaaaga actggtggag aaaaatattc cacaggagga agccgtagaa
     6001 cacctaatcc agctgatcaa ggataacggg gactggatag agccggatgc gaactaaaca
     6061 aacaaaaaag gtagtttata gctacctttt tgtttttatg ccaaagctat atgtaccggt
     6121 gtaggattag tcaggttatc gctaaccgga caacgtgctt caaccgtttg tatccatttc
     6181 tgtaatgtat ctttatcggt atcggtgtcg ggcttgattt ctacatgtat cgatttataa
     6241 cccgcacgat tttcggtctc ttttccaaac aatttctccg gattcaatgc tcccgatatt
     6301 tttatttcta ctcccttgag ttttatattc atttcctggg ccacaatatg gcacatgacg
     6361 ttaaggcaac cggataaagc ggccaacacg tattcaaccg ggtttgggcc atcgtttgtt
     6421 cctcccagat cggtaggttc gtctataatc atcttgaaag ttctcgcttt taccacagtt
     6481 tttgtgggat tttcactgtg cgcttttacg cggaatgtta attcttccat tttttctgtt
     6541 tttgaagttg tgatcttatt tttttgtttc tgcattttat gcggtgtaaa aataagaata
     6601 ataagtgtga aaaaatcatt aatcttttca attaaacatt tttcttttat tccgatcagt
     6661 ttaatgcatt ccggtttggt aatttgcaaa aaaagtagta atattgcacc cgctttcgac
     6721 gcaagaactg tcgaaaaggt cccatagctc agttggttag agcacctgac tcataatcag
     6781 ggagtccttg gttcaagccc aagtgggacc acagccctcg aaatttcctc cggaacgtcc
     6841 agtacgtacc ggaggttttc attttatgcc aaaaaaacag aagggaaaaa aagaatgtgt
     6901 atatttaccg ttaaaattaa gaaacggttg acatgattat tgcggttgat tttgacggga
     6961 ccattgttga gcacgcttat ccaaaaatag ggagacccat tccttttgcc atcgagaccc
     7021 ttcgcatgct tcacaacgac ggacaccggc tccttctctg gacggtacgc gaagggcagt
     7081 tgttacagga agccattgat tattgcgaac aaaacggcat ttactttttt gcagcaaacg
     7141 cgaattatcc cgaagaaaac cgggaagcct ctcctcgcaa attaaaggca gatttgttta
     7201 tcgatgatcg taatttagga ggactccccg attggggaat aatctacggg gcggtgcaag
     7261 ctaccgtgag gggagagtgc tcgtttgaag cctttataag caatactcct gttcccgcaa
     7321 agaaagaaaa gaaggggttc atgggatggc ttaagttgag gaatttattt attttttaag
     7381 ggcttaccgg tgattttatt aaaaaaacta cgaacgggat aatttgccat aaaccgatcc
     7441 agacctgtac ggtagcctgt gggaaatgat gccaagacaa gtagagatgc aatttcaatc
     7501 agattcctgt cgacaatcca gtaatttcca tctgcataaa gtccggccgc ggcactgcta
     7561 aaaggaggat aggtaaaata ataaagggat aatagtatga tgcccagcag tttggcaggc
     7621 ctttcgaaaa atcccaaaaa cagacacaat ccaattaaaa ttaaccccca tatatttaac
     7681 atgtcaatat aatgtattgc cttgtcggat tgcgcccaca tcttaaacaa agaagccagc
     7741 ggccctgtac tgtttaacag gaaagagcgc gatgaccaga catcgccgat atatttaaac
     7801 attccttcat agaggaaatg ccacccgatg actaccctta atgcagttag ggttataatt
     7861 tgcctggaag aatattttgt atccattttt ttaaccaata aggtattgtt cattttgttt
     7921 atcccagaaa ataggttttt tgtgtaaata tgataggttg gccagacaga aagtaacggt
     7981 ttcttcaaac ccctgatcaa tgccgcaact tacttttccc tgtcccctga tggcatctat
     8041 ccattctttt aggtgtaaga aagtcgcatc gatcaccttt ccatcgataa atgtagggcc
     8101 atagccgctt ttaaaataac tggtcgccgt tgcagaagtg atgccatcta cccctacgcg
     8161 ggcatttggc gcataataat aaaacggttc ttccgaatct tccacaatat ctttatatcg
     8221 ctgggagttt ggatctttat agagcataat tgcccgatct atttccattg tcgcttctga
     8281 gcccaatacc ttggtttgtc gataaatacc actttttaag gtgccatcgt aggtcatcgt
     8341 caatgctcta tccggataat agaatatagc gttgaatacg tcgggcaaat catcaatgcc
     8401 cacgttataa aagaaatggc cacccatcgc catcacagat tccggaatgc ccatttcgaa
     8461 gacttgattt acacaatcgt aagtgtgcga aaaatcattt cctatcatgc ccgtaccgta
     8521 atcgctatat ctttgccagt taaaatagcg tttactgtca aactcatgcc agggggcatt
     8581 gcacaaaaat cccttccagt ctattgtttg ctggtttccc aaatggtcaa attctctctc
     8641 ccggatccac gctccgaaaa gggagttcct gttggtaaat gtctggatca ttgatacgtc
     8701 gcccaatact cctttgcggt acaattccct tcccagttta taactcatct gttgtcgatt
     8761 ctcatggcct atctgaaaga cgatgttgga ggcgcttaca acttttttta attcgaccgc
     8821 ttcttctatg ctgtggcaca tcggtttttc taaatatacg tgctttcccg ccttcactgc
     8881 atctattgta atttgagcgt ggctatggtc gggtgttgcc acaatgaccg catcaatatc
     8941 cttgctgtgg atcatatcct gataggtttt gtaccgttta attccggacg agatttccgg
     9001 ggtactcctc tcttcccgga tattgtttct gcccacttct attcctcttt ccgcgtgaat
     9061 atcgaatgta tcgcagatac ccgcgatgtc cacatagagg ttttcatgtc ttttctgata
     9121 aggcagccac cggtcatatt gcccgtcagt cttcatctta tccacataat ccgggtgaaa
     9181 ataaccgaaa gagtttaaca gggattcgcc ccgccagccg tttccgataa gtcctatttt
     9241 tattcttgtt tgttcattac ccgtagagcc aggtaatctc tcgctcatca catcaaactg
     9301 gtcaatgtcc agcagtcgtt ttgaatcgac gcgacgatca tccaacactt tcagtatgtt
     9361 tttggtcgac cctattgaat atgcacccag gatgggtaca gttgaaatcc ccatcaaaaa
     9421 atcccttctt ttcatacgta cctctcttat tgaattttat tattgtgaat ctgaaatttg
     9481 caacatgact tttacacagt agctggtaca gtttagccaa tcaatatacc cgctttcctt
     9541 tttttggcgt tcattcaatt gccaataagt agggtaccgc cctgtgtttg cgtgttgagc
     9601 cactgttacg gcatttgcca actctatcat tttagcttta taaacctcat tttttgttgc
     9661 cttataaccg gtcaggtagg cttccatcat attggctacg cttgcattga tgggttcata
     9721 gcatgcatat tgttccaaca cacaaggggt tatccactct tctacgttgt actgcttctg
     9781 aggcattggt ttctcccaga taataaattg atcttcgcaa aaacgcaaca gatcatcagc
     9841 aactgaacga aattccttct tttgatgtat gttttgaaac aggtatattg caaaggcgct
     9901 tgcttcatct ttcgataaat ttttatacgg ttctaccggt gcaacatcct caaactgacc
     9961 ggaccagttg tatgtcttta acggattatt gatgatccaa tcataagctt tttttctcgt
    10021 atcgacatat tctgttacag catactgcga ttctaatcga tccagaaagt tgattatccc
    10081 aatggggata cagtcgtttt ccgttacggg agagccatct atccataatt tcagcttcca
    10141 ggttcccgat ggttcctgaa tttttttata cgtttgcgcg atttttaaag cagctt
//
LOCUS       DAOSDR010000053        10007 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrimonas sp. isolate AMR_MDS_1826
            NODE_2551_length_10007_cov_3.718348, whole genome shotgun sequence.
ACCESSION   DAOSDR010000053 DAOSDR010000000
VERSION     DAOSDR010000053.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863355
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrimonas sp. (wastewater metagenome)
  ORGANISM  Petrimonas sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Dysgonomonadaceae; Petrimonas.
REFERENCE   1  (bases 1 to 10007)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10007)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:10:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,720
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 35
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10007
                     /organism="Petrimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2551_length_10007_cov_3.718348"
                     /isolate="AMR_MDS_1826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2023866"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..210)
                     /locus_tag="PLI30_03420"
     CDS             complement(<1..210)
                     /locus_tag="PLI30_03420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019538162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoPQ-activated pathogenicity-like protein PqaA
                     type"
                     /protein_id="HOI78637.1"
                     /translation="MKRLLFILTVYFCVIVGFGQQAITPETALGAYVRNDDPTWGWEV
                     RNRYPIDDTEVYSLLFISQKWHGMLW"
     gene            complement(259..2439)
                     /locus_tag="PLI30_03425"
     CDS             complement(259..2439)
                     /locus_tag="PLI30_03425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019538163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI78638.1"
                     /translation="MKLNQIGMLICLLFIKSAFAGTVNLPVKDQPYTVPVSIKYTLSP
                     KWKDVRFRKLVLDGDNSVFILTDAGLFRDFPGEIISKDLRYASLADKYPVDICVQEGT
                     GYLYYLYPDRFLTNKHAGAICGLLPENKYSHVAVNSKDDVLLIGERTGVMYNRNTKVR
                     ELGLPHDGLVRVYVSDDQFWYLSATGIYWLDRDKWVRIHSGKNLTAMAFNGDKIAVGT
                     TDGYYVIDRSGKIVESHNNKLPVPLINVMLAVDGKYWFATDQGAFVKQPGGFDYYASK
                     RWLDQDKIIDMAADSDGNLYFLTPAGLNKVQFEQETLAEKAAFLQDNLRKYHLRYGFS
                     SASRLVDPDDPATLRLDDDDNDGLWTSFYLGSQAFRYAVTKEEKAKEYVWESFEAFER
                     LLTIHPIEGFSARTFERTGYIAGDTIPWRPAPDKNWWWKGTTSTDEFVGYLFVTSVID
                     QFVAQTPEEKKRVADYFDAIMTHIIANDYYFIDHDGKPTLWGRWNPEYVNSFATTQFD
                     RRLNSTLTITGLQLAYKLTGKEIYKSEAFRMMEEHGYLDNMKIPMGNIRFTPGFQHQG
                     ITMGEDWNHSDDEMAFLTYWVLYHYAFDDTLKREYAKMIRDHWEIEKPERNALWNLLA
                     YGTSGDIDLESTLWYLQGFPKDRRRYVVKNSHRKDLEFLPKDASTNFREQTTTQLIPK
                     RERPLNRHNNNEFIIDGGRGTHQQLTGDEFLLPYWMARYLDVIR"
     gene            complement(2505..4175)
                     /locus_tag="PLI30_03430"
     CDS             complement(2505..4175)
                     /locus_tag="PLI30_03430"
                     /inference="COORDINATES: protein motif:HMM:NF024181.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SusD/RagB family nutrient-binding outer membrane
                     lipoprotein"
                     /protein_id="HOI78639.1"
                     /translation="MYTLKKQFYLFLTLLLGLVVSCRDLDELNINPNGVDPAIADVNF
                     LLPTVETNVGQTVYNIGFGTFSGIMQHTQQTGWFGGYNNYDWNTLSHSWNGFYSILMN
                     NDEYFRKAVEHNYEFHEGVARIMRAYVFGLITDIWGDAPYKGALKAEQGSAHFMPVFD
                     PQQEIYHGILADLDTANTLLSKSANEYRNIVPKQDVVYRGDVSKWQKFANSLALRYYM
                     RLQAKEPAFAEEGIKRIAGDPAKYPLIRVAGDDANISFPGTSASVSNPLNIVYDAKPD
                     DGAYMRVKMGKTLVDIMRDYDDPRLGVWANKIEIPLKLVSGTGIDRIVEIDDAHKVRE
                     VSQDKVTAFEANGMKVDFNPEYAGVTISHPLVQIFNMNIANGSQGTVNPHASHLNDRY
                     TKTTDPLVLMRLISAAEINLILAEAAYRGWISGAAAGYYTDGVRESFRAWGVEAEFGN
                     YIENASYDGLESIIEQKWIAHWSTAQEAWFDWRRTGLPALQTGPAAMRPVIPVRWYYH
                     TADEIDKNRVNAEAAIGRLEATPYQGTDQSKNSAWSKMWLLQGTGKPW"
     gene            complement(4189..7617)
                     /locus_tag="PLI30_03435"
     CDS             complement(4189..7617)
                     /locus_tag="PLI30_03435"
                     /inference="COORDINATES: protein motif:HMM:TIGR04056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0071702 - organic substance transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SusC/RagA family TonB-linked outer membrane
                     protein"
                     /protein_id="HOI78640.1"
                     /translation="MRNLQKDQNKGLYTVIISVALLILFMGNGYAETTDTRQNIVNVT
                     GTVTSATDDMPLIGVSIVVKGTAEGTVTDMEGNYTINVPSNATLVFSYIGYITQQIAV
                     VGRTNISIVMEESTETLSEVVVTALGIGREEKSLGYSVGKLGSEELAKVVHENVLTSM
                     AGKVTGVTINSTGGTGSTVSMIIRGATSMSTDNQPLFVIDGVPMGSSVNNVGGFGSGN
                     LVDYGNSISDIDPESIESISILKGPSAAALYGTRAGNGVVLITTKKSDRKGGMKVEVV
                     SNTVFDIPSKYLDLQNRFGFGARSYTDTSFPGGILPPYSPSEQLGAGPELNKGYWQVQ
                     PFAPLDANGVPVPTELVSYPDNYKNFINGSAITTTNSVAISSSSDRVNYRLGVSNMTN
                     KGLVPNSDLNRNNLTLAATSEVVKGLNVSTDINYSQNWADNRPATQERGANPLQWAAW
                     TPPNVDIRKMKDYKLGGNEVLTIGKGWNNPYFLAYGINNSFKRNRLIGNVSATWEITS
                     QLSLMGRYALNKSDETRETKIDPGYTGEPNQGAYGIVTSESTETNADVLATYKNHWND
                     FSFAASAGGNLLYSHWANVSNSSKPGSGLIIPYLYTIQNIESSSLNYSNARYERAINS
                     LYATANLGWKDMVYLDLTARNDWASTLPAENRSYFYPSASLSLLLNNLIDMGKNVNLF
                     KIRGGIAQVGNDTSPYSLYATYYNAGQWGDAIRLGKPGNLLNPILLPEESKSYEVGMD
                     LKIFNNRLRFEGTWYRADNRNQILSVPLAGSAGFNSIKINAGLLQSKGVELMLGFTPI
                     ETKDWRWDLNMNFTKNKTWLLELSEGTDFVEFWNEARVRNVAYAKDESLGRDGLIGNL
                     YTRKILRVTDKNSEYYGYPLLPENDEDAEWQSEDDYSKGGNYNPNFIMGVQTSITYKN
                     FTLSMTFDWRSGGQYVSQTWRYLTEGVVSNTWLNQLVTPPEGLGGGPSQGLRDWVVAN
                     AERLIYTNNPRPVGGPTPDFGGYYNEFYTGIGAYDGVFAPGVYGRYDDNGNFILIKEN
                     LGNEGTEFRPYAMSYPWDIGEANIFDADYIKLREIALNYRVPDKTAQKLKMRDLNISV
                     YSRNIMLWAKSAGMGIDPERAYQSSGNGSFKQGVERFNAEPWVVPVGFKLSFTF"
     gene            complement(7640..8653)
                     /locus_tag="PLI30_03440"
     CDS             complement(7640..8653)
                     /locus_tag="PLI30_03440"
                     /inference="COORDINATES: protein motif:HMM:NF012999.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HOI78641.1"
                     /translation="MKTRFLYLTSLFLCMTAFFSSSCSSEETGDDLPLSALIFHSIDG
                     KQAALTALTHSAVSWEWDFGDGNTSNEKDPVHVYGEGGYYKVKLTAKDNAGNSVTAQV
                     SLAVALTPYALLTGDHTAEGYDGKTWRLTQSHTVNDKLVNSDANFSLLAPKIPSLPQG
                     AFDVYVGLKEAYNDEFTFYYDGSYKHSTSDGTSFGGIVYAMILQQMGSSQITKVGGKA
                     ALGADLFALSTYTPDKNTTFVLNENDDFTVPTITKFATGTQPPGIPVVTYPDVMTLDF
                     PNSNAFIGIRDFHRRVIMQEITNNTMRLVMFMTLDPKAIFSQNPLIALSTSAAILTFE
                     AIN"
     gene            complement(8784..9695)
                     /locus_tag="PLI30_03445"
     CDS             complement(8784..9695)
                     /locus_tag="PLI30_03445"
                     /EC_number="4.1.3.3"
                     /EC_number="4.2.1.41"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019538167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009436 - glyoxylate catabolic process
                     [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HOI78642.1"
                     /translation="MRKIVQGIVTPLVTPLIDNNTLDIAGLERLIEHVISGGVHGIFI
                     LGTTGEASNLAINLRIEMIRQSSRLIRKRVPLLVGISDTSITESIRLAETAASFSADA
                     VVSTPPFYFVMDQTELAEYYESLVPQLNLPLYLYNMPVNTKISFDPKTIKRIAENPRV
                     VGFKDSSGNAPYLQRVIHEMRNCENFSIFVGPEEMTAEMVLLGADGGVNGGANIFPRL
                     YVDLYHAAVANDIEVVRNLQAKIMNISSTLYTVGENGSSYLKGLKCSLEILGICNGFI
                     ASPFKKFSGQQYGQVKTALEKIMRICE"
     gene            complement(9827..>10007)
                     /locus_tag="PLI30_03450"
     CDS             complement(9827..>10007)
                     /locus_tag="PLI30_03450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI78643.1"
                     /translation="IVILMLTRIQGSPFSSHPLLQVDSRKLFKVLVAVILFITVMSFI
                     AVNLHEGLPGTQNRF"
BASE COUNT         2515 a         2525 c         2155 g         2812 t
ORIGIN      
        1 ccatagcatc ccatgccatt tctgagagat gaacagcaag gaatagacct ccgtgtcatc
       61 gattgggtat ctgttgcgca cttcccatcc ccacgtagga tcatcgttgc ggacataggc
      121 acccagtgcc gtttcaggcg taatcgcttg ttggccaaat ccaacaataa cgcaaaaata
      181 gacagttaaa ataaacagta atctcttcat acgttcaatt ttatttttta gttttataat
      241 gaattacaca ccatcacgtc atcgaatcac atcaaggtaa cgcgccatcc aataaggcaa
      301 cagaaactca tcgccggtaa gctgctgatg cgtgcccctt cctccgtcaa taataaattc
      361 gttattatta tgcctgttca gcggccgttc ccttttaggg ataagttggg tagttgtctg
      421 ctccctgaag ttggtgctgg catctttcgg cagaaattcc aggtctttcc ggtgtgagtt
      481 tttcaccaca taacggcgac ggtcttttgg aaacccttgc agataccaga gggtggattc
      541 cagatcgata tcacccgatg ttccataggc cagcaggttc cagagtgcat tgcgttccgg
      601 tttctcaatc tcccaatgat cgcgtatcat tttcgcatat tcccgcttta aggtatcgtc
      661 aaacgcataa tggtataaca cccaataggt aaggaatgcc atctcatcat cagaatggtt
      721 ccagtcctct cccatagtga ttccctggtg ctggaatccc ggggtaaaac ggatattgcc
      781 catcggtatt ttcatattat ccagatatcc atgctcttcc atcatacgaa acgcctcact
      841 cttatagatc tcttttccgg tgagtttata ggccaattgc aaccccgtga tggtcaatgt
      901 gctgttcaac ctgcggtcga actgggtggt ggcaaaagag ttcacatact cgggattcca
      961 tctgccccag agtgtgggtt taccatcatg atcgataaaa taataatcgt tggcgatgat
     1021 atgggtcatg atagcatcga aatagtccgc cacccgtttt ttctcttccg gcgtctgcgc
     1081 gacaaactgg tctatcacgg aagtgacgaa cagataaccc acgaactcat cggtgctggt
     1141 ggttcccttc caccaccagt tcttgtcggg ggcgggtctc caggggatgg tgtctccggc
     1201 tatataacct gtccgctcga atgttcttgc cgaaaatccc tctatgggat ggatagtcag
     1261 taatctttca aaagcctcga atgattccca tacatactct ttggctttct cctctttggt
     1321 gacggcataa cggaatgcct gacttcccag atagaatgag gtccataaac cgtcattgtc
     1381 atcatcgtcg aggcgcaggg tggcaggatc gtccgggtcg accaaccgcg aagcggatga
     1441 gaaaccataa cggagatgat acttgcgcag gttgtcctgc agaaacgctg ctttttcagc
     1501 cagtgtttcc tgctcaaact gcaccttgtt caaaccggcc ggggttaaaa agtaaaggtt
     1561 tccgtcgctg tccgcggcca tatctatgat cttgtcctga tcgagccaac gtttggaggc
     1621 gtaatagtcg aaccccccgg gttgtttgac aaaagcgccc tgatcggttg caaaccaata
     1681 ctttccatcc acggccaaca tcacgttaat aaggggtacg ggaagcttat tgttgtgaga
     1741 ttcaacgatt tttccgctcc ggtcaatcac gtaatagcca tccgtggtac ccacggctat
     1801 cttatcccca ttgaatgcca ttgctgtcag gtttttaccg gaatggatcc tcacccattt
     1861 gtcacggtca agccaataga tgcctgtggc agacagatac caaaattggt catccgacac
     1921 atacaccctg accagcccat catgaggaag acccaactcc ctgaccttgg tgtttcggtt
     1981 atacatcaca cccgtccgct cccctatcaa caagacgtca tccttgctgt ttacggccac
     2041 gtgactgtat ttgttttcag gcagcaggcc gcatattgct ccggcatgtt tattggtaag
     2101 gaaacggtcg ggatacagat aatagagata accagtccct tcctggacac aaatatcgac
     2161 aggatattta tctgccaggg aggcgtaccg gagatccttg gaaatgattt cccccggaaa
     2221 atctcggaac aggccggcat cggtcagtat aaaaacgctg ttatcgccgt ccaatactaa
     2281 tttcctgaag cggacatctt tccatttagg agataatgta tacttgatcg ataccggaac
     2341 cgtataaggt tggtccttca ccggcagatt gactgttccc gcaaaagcag attttataaa
     2401 caagagacat atcaacatgc ctatctggtt taatttcata cgattataat tttaaaataa
     2461 gaattaagat catttcaagt cttttgttcc tggttctact tatcctacca cggcttgccg
     2521 gttccttgga gaagccacat tttcgaccat gcgctgttct tggattgatc ggtaccctga
     2581 tacggcgttg cttccagtct tccgatggct gcctcggcat ttaccctgtt tttatcgatc
     2641 tcatcagcgg tgtgataata ccatcggaca ggaatcaccg ggcgcattgc cgccggacct
     2701 gtctgcaaag cgggcaagcc ggtcctgcgc cagtcgaacc aggcctcttg tgctgtactc
     2761 caatgagcta tccatttttg ttcaataata ctttccagcc cgtcgtaaga tgcgttctca
     2821 atgtaattgc caaattcagc ttccactccc caggccctga acgattccct cacaccgtcg
     2881 gtataatatc cggcggcagc acctgaaatc catccccggt atgccgcttc ggcaagtatc
     2941 agattgattt ctgcagcaga tatcaatctc atcagcacaa ggggatcggt tgtcttcgta
     3001 taccggtcgt ttagatggga agcatgggga ttaacggttc cctgtgagcc attggcgatg
     3061 ttcatattga aaatctgcac caacggatgg gaaatggtca ctcccgcata ttccgggtta
     3121 aaatccacct tcatgccatt ggcttcaaaa gcggtcacct tatcctgtga aacttcgcgg
     3181 accttatgtg cgtcatcaat ctcaacgata cgatcgatcc ctgtccccga aaccagtttc
     3241 agcgggattt ctatcttatt ggcccataca cccaagcgcg gatcatcgta gtccctcatt
     3301 atgtccacca atgtctttcc cattttcacc cgcatgtagg caccgtcgtc cggcttggca
     3361 tcgtaaacga tgtttaacgg gttggaaaca ctagctgacg ttccggggaa gctgatgttc
     3421 gcgtcgtcgc cggccaccct gatcaacggg tatttggcag gatctccggc aattctcttg
     3481 atcccttctt cagcaaatgc gggttctttg gcctgtaatc tcatatagta ccgtaacgcc
     3541 agtgaatttg caaatttctg ccatttggag acatcgcccc tgtacaccac atcttgtttg
     3601 ggcacgatat tgcgatactc attcgcactt ttcgacaaaa gcgtattcgc tgtgtccagg
     3661 tcggcaagga tgccatggta aatctcctgt tgcggatcga aaaccggcat aaaatgggct
     3721 gatccctgtt cggccttgag agcgccttta tagggggcgt ctccccatat atcggtgatc
     3781 aggccgaaga catacgccct catgatccgc gccacaccct catggaactc ataattatgc
     3841 tctactgcct tcctgaaata ctcgtcattg ttcatcaaaa tgctgtaaaa gccgttccag
     3901 ctatgcgaaa gggtattcca atcgtaattg ttgtaacctc cgaaccagcc tgtctgctgc
     3961 gtatgttgca taattcccga aaaggtaccg aagccgatat tataaacagt ttgtcccaca
     4021 ttggtttcga ccgtgggcaa caggaagttt acatccgcga tggcgggatc cacgccgttc
     4081 ggattgatat tcagttcatc cagatccctg caggaaacga ccaatccgag gagcagcgtt
     4141 aaaaaaagat aaaactgttt ttttaatgta tacatgacat tctttcgatt aaaaagtaaa
     4201 acttaattta aacccaaccg gaacaaccca aggttcggcg ttaaaacgtt ccacgccttg
     4261 cttgaaactt ccgttaccac tcgattgata ggccctttcc ggatctattc ccatgccggc
     4321 gcttttcgcc caaagcatga tgttccggct gtataccgaa atgttcagat ctctcatttt
     4381 cagtttttgg gctgtcttgt cgggaaccct gtagttgagc gcgatctctc ttaatttaat
     4441 gtaatccgcg tcaaaaatat tggcttctcc gatatcccag gggtagctca tcgcataggg
     4501 tctgaattcg gtaccctcat tgccaagatt ttccttgata agtataaaat taccgttatc
     4561 gtcataccgg ccatacacac caggagcaaa aaccccgtcg taggccccga taccggtata
     4621 aaattcatta tagtatccac cgaagtcggg ggtaggtcca ccgaccggcc ttggattatt
     4681 ggtataaatc aacctctcgg cattggctac cacccagtca cgaagtccct ggctcggacc
     4741 accccccaat ccttcgggcg gcgtaaccaa ctggttgagc caggtattcg acacgacccc
     4801 ctcggtcagg taccgccagg tttgggacac atattgtccg cccgaccgcc agtcgaacgt
     4861 catgctcaaa gtaaaattct tgtaggtgat agaggtctgg actcccataa tgaaattggg
     4921 gttgtaattg ccccctttgg aatagtcgtc ttcagactgc cattcggcat cttcgtcgtt
     4981 ctccggtaac agcggatagc cgtaatattc agagtttttg tcggtcaccc tcaagatttt
     5041 ccgggtataa aggttaccga tcaacccgtc cctgcccaga gattcatctt ttgcataagc
     5101 aacattcctc accctggctt cattccagaa ttcgacaaaa tcagtacctt ccgacagttc
     5161 cagcaaccat gtcttattct tcgtgaaatt catattcaga tcccatctcc agtctttggt
     5221 ttcaatgggt gtaaagccaa gcatcaattc cacgcctttg ctttgcaaga gacccgcatt
     5281 gatctttata ctgttgaacc ccgcagatcc ggccagcggc acgctaagaa tctggtttcg
     5341 gttatccgcc cggtaccagg tcccttcgaa ccgcaaccgg ttattgaata tcttcagatc
     5401 catccccact tcataggatt tcgactcttc gggaagaagg ataggattta ggaggttccc
     5461 cggctttcca agacggatgg cgtcacccca ttgacctgca ttataatagg ttgcatatag
     5521 gctgtaaggc gaagtgtcgt ttcctacttg ggcaatgcca cctcttatct tgaaaagatt
     5581 tacgttcttt cccatatcga ttaaattatt cagcagcagg ctcaatgagg cggaaggata
     5641 gaaataggat ctgttttctg caggaagcgt gctggcccag tcgtttctcg ccgtcagatc
     5701 aaggtatacc atgtccttcc atcccagatt agccgttgca taaaggctgt tgatcgctct
     5761 ttcgtaacgg gcattggaat aattcagtga gctgctttca atattctgga tcgtatacag
     5821 ataggggata atcagtcctg agcccggttt ggatgagtta ctgacattcg cccagtggga
     5881 ataaagaaga ttccctcccg cagaggctgc aaaggagaag tcattccaat ggtttttata
     5941 ggtagccagt acatcagcgt tggtttcagt gctttcgctg gtaacgatac catacgctcc
     6001 ttgattgggt tcaccggtat atccgggatc tatcttcgtt tcccttgttt cgtccgactt
     6061 gttcagcgca tatcttccca taaggctcaa ttgcgaggtt atctcccagg tagcggaaac
     6121 attcccgata agcctgttcc ttttaaaact attattgatg ccgtatgcaa ggaaatatgg
     6181 attattccag cctttcccaa tagtaagcac ttcgttgcct cccagtttat aatctttcat
     6241 cttgcggata tccacattgg gcggtgtcca tgcagcccac tgcagaggat tggcacctcg
     6301 ttcctgcgtg gccggcctgt tatcggccca gttctgtgag tagttgatat cggtacttac
     6361 attcagccct tttactactt ccgatgtggc cgcaagggtc aggttattcc tgttaaggtc
     6421 cgagttgggc accaaacctt tattggtcat attggatacg ccgagccgat aattcaccct
     6481 gtcgctgctg cttgatatcg ccacactatt ggtagtagtg atggcactac cattaataaa
     6541 attcttatag ttatcgggat aggaaaccaa ttccgtaggt accggaacac cgttggcatc
     6601 gagtggtgca aacggctgaa cctgccagta acccttgttc agttccggtc ctgccccaag
     6661 ctgttccgaa ggactgtaag gaggaaggat ccctccggga aaggaggtat cggtataact
     6721 cctggcacca aagccaaagc ggttctgcag gtccaggtat tttgacggga tatcgaaaac
     6781 ggtatttgaa accacttcca ctttcattcc gcctttgcga tccgattttt tcgtagtgat
     6841 aagcaccact ccgttaccgg cacgtgtgcc atacaaggcc gctgcacttg ggcctttgag
     6901 gatggaaatg ctttcgatac tttcgggatc gatatccgaa atggaattcc cgtagtcgac
     6961 cagattgccg ctgccaaaac ctccgacatt gtttacggaa ctgcccatcg gcaccccatc
     7021 gatcacgaac aaaggctggt tatcggtact catcgaggta gcccccctaa tgatcatgct
     7081 gactgtagat cctgtacctc cggtggaatt gatggtcacc cccgttactt ttcccgccat
     7141 cgaagtgagt acgttctcat ggaccacttt ggcaagttct tcggatccaa gtttccccac
     7201 cgaataccca agtgattttt cttcacgacc gataccgagg gctgtaacca ctacttcact
     7261 cagcgtttca gtactctctt ccatgacaat gctgatattc gtacggccta caacagctat
     7321 ctgttgtgtt atatacccta tataggaaaa aacaagtgtt gcattggacg gtacattgat
     7381 tgtgtaattc ccctccatat cggtcacagt accttcggct gttcccttta cgacgatgct
     7441 gacgccgata agcggcatgt cgtcggtagc tgatgtaacc gtacccgtaa catttacaat
     7501 gttttgccgg gtatcggttg tctctgcgta gccattaccc ataaagagga taagtaaggc
     7561 aacagagata ataactgtat acagtccttt attttgatcc ttttgcaaat ttctcatgtc
     7621 taaattgtct gaataggttt taattaattg cttcgaatgt tagaattgcg gcagaagtac
     7681 ttaaggcaat caaggggttt tgactgaata tagccttggg atccaaagtc ataaacatga
     7741 ccaaccgcat ggtgttgttc gtgatctcct gcataattac tcttcggtgg aaatccctta
     7801 ttccgatgaa tgcattcgag ttgggaaaat ccaatgtcat cacatccgga taggtcacca
     7861 caggaattcc gggaggttgc gtgcctgtag caaacttcgt gatggtaggg acggtgaaat
     7921 catcattctc attcaataca aatgtcgtgt ttttatcagg cgtgtaggtt gacaaagcga
     7981 aaagatcggc ccctaacgcc gcttttccac ctactttggt aatttgggat gatcccatct
     8041 gttgcaagat cattgcataa acaataccac cgaaagaggt accatcggat gtgctgtgtt
     8101 tgtaactacc gtcataataa aaagtaaatt cgtcattata ggcttctttg agtcccacat
     8161 atacatcgaa tgctccctgg ggaagtgacg gaatcttggg tgcaagcaat gaaaaatttg
     8221 catctgaatt aaccaatttg tcattgacgg tatgcgactg tgtcaacctc caggttttgc
     8281 cgtcataccc ttcagccgta tgatctcctg tcaatagggc atagggagtg agcgcaaccg
     8341 ccaaactcac ttgcgcggtt accgaattgc cggcattgtc ttttgccgtt aatttaacct
     8401 tataataccc cccctcccca tacacatgca cgggatcttt ttcgttgctg gtatttccgt
     8461 ctccaaaatc ccactcccag cttaccgcac tgtgggtaag cgctgtcaat gctgcctgtt
     8521 tcccgtctat actgtggaaa atcaaggcag aaagcggtaa gtcatcccct gtctcttcac
     8581 ttgaacacga tgaggaaaaa aaagccgtca tgcacagaaa gagagaggtt aagtacaaaa
     8641 aacgtgtttt cataaaaact tctttactga tcaattttga ttaacttaaa ttcgttttaa
     8701 tccattcgcc aataacgaca atgccgtttc tacagtatca ctctcggatt attgctaatc
     8761 caggtttatc catccttttc ggatcactca cagatcctca taatcttttc cagggcagtt
     8821 ttaacttgcc cgtattgctg cccgctgaat tttttaaaag gggaagctat aaaaccgttg
     8881 caaattccca gtatctccaa tgaacatttc aatcctttca ggtaactgga accgttttca
     8941 ccaacagtat aaagggtaga agagatattc attattttgg cctgcaggtt tcttaccacc
     9001 tcgatgtcat ttgctacagc agcatgatac aagtccacgt acaaccgtgg aaaaatattt
     9061 gctcctccgt taactcctcc gtcagcaccc agcaaaacca tttcggctgt catttcttcc
     9121 ggccccacaa atattgaaaa attttcacag ttacgcatct cgtgtataac cctctggaga
     9181 taaggcgcgt tacccgagct gtccttaaac cctaccacac ggggattttc ggctattctc
     9241 tttatcgttt tggggtcaaa gcttatcttg gtgtttacag gcatattgta caggtatagc
     9301 ggtagattta actggggtac cagcgattca tagtattctg ccaattccgt ctgatccatg
     9361 acaaaataaa acggaggggt ggaaactact gcatctgcac tgaaactagc tgctgtttca
     9421 gccaaccgga tactttccgt aatggaagta tctgaaatac cgaccagcag gggcactctt
     9481 tttcgtatca acctgctcga ctgacgaatc atttcaattc ggagattgat ggccaggttt
     9541 gaggcttctc ctgttgtccc caaaataaaa atgccgtgta caccacccga tatcacgtgt
     9601 tcaatcaatc tctccaaacc ggcaatatcc agggtgttgt tgtcgataag cggagttaca
     9661 agtggggtta ctattccctg aacaattttt ctcatatgat ttaaattatt tattttcaaa
     9721 ttataagcaa gacatttact cacttcagca taacaggagt aaaccagcct taggctcctt
     9781 tcatgtatct aaacttttag ttgtgcaaaa aattgttcgc atgaatttag aaacgatttt
     9841 gtgttcccgg caatccctcg tggagattaa ctgcaataaa cgacatgacc gtaataaaaa
     9901 gtatcactgc gacaagcacc ttgaagagtt tccgggagtc aacctgtaac aaaggatgtg
     9961 atgaaaaagg acttccctgt attcgtgtca acatcagtat cacaatt
//
LOCUS       DAOSDR010000054         9962 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrimonas sp. isolate AMR_MDS_1826
            NODE_2563_length_9962_cov_4.645604, whole genome shotgun sequence.
ACCESSION   DAOSDR010000054 DAOSDR010000000
VERSION     DAOSDR010000054.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863355
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrimonas sp. (wastewater metagenome)
  ORGANISM  Petrimonas sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Dysgonomonadaceae; Petrimonas.
REFERENCE   1  (bases 1 to 9962)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9962)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:10:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,720
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 35
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9962
                     /organism="Petrimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2563_length_9962_cov_4.645604"
                     /isolate="AMR_MDS_1826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2023866"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(8..80)
                     /locus_tag="PLI30_03455"
     tRNA            complement(8..80)
                     /locus_tag="PLI30_03455"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(45..47),aa:Lys,seq:ctt)
     gene            complement(183..449)
                     /locus_tag="PLI30_03460"
     CDS             complement(183..449)
                     /locus_tag="PLI30_03460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006841918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HOI78644.1"
                     /translation="MNKSELVSAIAEKSGLSKVDAKKALDATLDAISGEVKKGGKVVL
                     VGFGTFSLTQRSARKGINPRTKKAINIPAKKAAKFKAGAALSNL"
     gene            652..1416
                     /locus_tag="PLI30_03465"
     CDS             652..1416
                     /locus_tag="PLI30_03465"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005867662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HOI78645.1"
                     /translation="MMNNLRSGFGAALPPVTLNIIIINVILWLAQVVFLRQGINLAEL
                     FGLHYIASEGFRVYQLVTYMFLHDSGSFMHVFSNMFAVFMFGRTLEHVWGSKRFLSFY
                     LVTGVGAGLVQLVVAFLRIQSVKSGMSVEAIEEVYSQGYQLLQQNMNYINSVQGSLNI
                     ALNSVTVGASGAVFGILLAFGMLFPNAELFIIPFPFPIKAKWFVLGYGVFELIFGISN
                     FSWDNVAHFAHLGGMLFGIFMILYWRKKDRVHGNYY"
     gene            1400..2287
                     /locus_tag="PLI30_03470"
     CDS             1400..2287
                     /locus_tag="PLI30_03470"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019540160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HOI78646.1"
                     /translation="MATIIDRLKYRYKNGDVLVKFVFINAAVFLILKVVGIFFVLFNI
                     SLVDLVSFLGVPSELSQLLNRFWTLLSYMFVHEQFWHLLFNMLWLYWFGRIFLQYFSG
                     RTLGSLYVLGGFAGAFLYIVAFNTVPYFVGMGHSWMIGASAAVMAVVMGAAFYRPNVQ
                     LNLLLIGQVRIVYIAVGVFLIDFLSLGNGINEGGHVAHIGGAIAGYIFARQYRNGRDI
                     TGWVSKLLDGLANLSKPRRKKTKMKVEYRRRETDREYNERKHNEQAEIDAILDKLKKS
                     GYSNLSSEEKKKLFDASKK"
     gene            2271..3395
                     /locus_tag="PLI30_03475"
     CDS             2271..3395
                     /locus_tag="PLI30_03475"
                     /inference="COORDINATES: protein motif:HMM:NF015338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HOI78647.1"
                     /translation="MPVKNKIALFRGIVRNIIFATNIIAILLLFSSFLSWRVSPLKTN
                     LFSYIGIAFGFVFFLNISYLFLWIAFKKWKLAFFSLVSLLLCYHPIATFFPMNIFPEK
                     VPRNSLKILTYNVEGFVNENKKEDKEHPILDYIVETDADIVCLQEYLVSKTGQSIKSQ
                     RDVNRILNKYPYHSSTALEASGKYHIYGLACFSKYPIEKTHEVVFNSSFNGAAVYTID
                     VNGKKLAVANVHLESNSISAEDKKLYGDFIQNSDEVNLEDVTSNIRSRLGRAYRMRAE
                     QVRKVKDHLSELNVDGTLICGDFNDTPISYVYAQMKEGMDDAFASTGFGPGITYHKDF
                     FWFRIDNIMHSPNLKSYKAKVDKVPYSDHYPLTTFLNVGD"
     gene            3478..3840
                     /locus_tag="PLI30_03480"
     CDS             3478..3840
                     /locus_tag="PLI30_03480"
                     /EC_number="4.1.2.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006841749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070497 -
                     6-carboxy-5,6,7,8-tetrahydropterin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase"
                     /protein_id="HOI78648.1"
                     /translation="MYKISKQFAFSAAHSLDGLPGDHPCTRLHGHNYVVTVHLRSKEL
                     NDTGFVKDYNELKVVKQYIDEHIDHRNLNDIMYPLNSSAENLARMFFDIFKPMLPELY
                     AVEVSETPKTSAIYELDN"
     gene            3824..4405
                     /locus_tag="PLI30_03485"
     CDS             3824..4405
                     /locus_tag="PLI30_03485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019540157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase QueE"
                     /protein_id="HOI78649.1"
                     /translation="MSLIINEIFYSLQGEGGRTGEASVFIRLSKCNLACSFCDTDFKS
                     GKSMALDEIYAEISSYPCRWIIWTGGEPTLQLNEEIVAFFKDKGYRQAIETNGTRRVP
                     SGIDYITCSPKQQFGKIRILIPEVDELRFPMAKGDPLPDVSVLPKTRRYLLSPIFDGM
                     NVIQENVDYCVELIKQNPHWGLSLQVHKLIGIR"
     gene            4431..5345
                     /locus_tag="PLI30_03490"
     CDS             4431..5345
                     /locus_tag="PLI30_03490"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008773932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016891 - endoribonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0042779 - tRNA 3'-trailer cleavage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HOI78650.1"
                     /translation="MTRFEITILGCGSAMPTTLHNPSSQLVNVNEKLFMIDCGEGTQL
                     QLRKYKTRINKLHSIFISHLHGDHVFGLPGLLATLSLLGRTGDLNIYAHKDMELFLDP
                     FIKHFGNQFPYKINLTPLDPYQHQLIFENNSIRVFSFPLKHRIPTSGFLFEEKDRPRH
                     IKREMIDFYNIPVCKINEIKKGGDYLTDDGKWIKNEILTTPAIPPRTYAYCSDTAYSP
                     EIIPYIQHSDVLYHEATFAEAENLRAQETYHSTARQAAEIARSANVRKLIIGHFSSRY
                     HELGELLDEAQAVFPETELADEGMTINL"
     gene            5455..5775
                     /locus_tag="PLI30_03495"
     CDS             5455..5775
                     /locus_tag="PLI30_03495"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOI78651.1"
                     /translation="MKRFYWTTLCILLFLCGFGIDGIYAQDQNRKEVMEEVVPETKIK
                     VTENRLVIENLPKDGVLEIFSIMGVKVYTRKIKAGTNEYQLDLPKGYYIIRIGDLVKK
                     ILLK"
     gene            5903..6901
                     /locus_tag="PLI30_03500"
     CDS             5903..6901
                     /locus_tag="PLI30_03500"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019540145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HOI78652.1"
                     /translation="MKIAIVGTSGAVGQELLRVLDARNFPVDELFLFGSSRSAGSTYT
                     FRGKEITVKELKHNDDFKGIDIAFVSAGGGTSLEFAETITRYGTIMIDNSSAFRMDDD
                     VPLVVPEVNAEDALKRPRNIIANPNCTTIQMVVALKAIEDISHIKRVHVSTYQAASGA
                     GASAMAELQEQHRQLVNNENPTISKFAYQLAYNLIPQVDVFTENGYTKEEMKMFNETR
                     KIMHSNIEVSATCIRVPVLRAHSEAIWIETERPVSVEEAREAFAKAEGVVLLDSPEKK
                     VYPMPLDAAGQDPVYVGRIRKDLTNPNGLTFWTAADQICKGAALNAVQIAEYLMKQ"
     gene            complement(6915..7400)
                     /locus_tag="PLI30_03505"
     CDS             complement(6915..7400)
                     /locus_tag="PLI30_03505"
                     /inference="COORDINATES: protein motif:HMM:NF016213.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF456 family protein"
                     /protein_id="HOI78653.1"
                     /translation="MLPEPLLISLAVICVIVGAVGTVVPVLPGVPLCWAGLLALKFVP
                     SVKDNVSWTVIVLLGIITIGVTILDNILPLWGTRKMGGNKKVVWGATIGLLIGFFLGP
                     WGIIFGPFAGALVSGLIVGNKIKPAVKHASGAFFGYIAGLIIKLITVGLIVFFFVMTL
                     V"
     gene            complement(7404..8186)
                     /locus_tag="PLI30_03510"
     CDS             complement(7404..8186)
                     /locus_tag="PLI30_03510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004307814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HOI78654.1"
                     /translation="MNPIIEIHNLNAGYENKPDVLKNVSLTVFENDFLGIIGPNGGGK
                     TTLLKAILGLIQPSSGNITFLDAGKKVSHLNIGYLPQINQIDKKFPISVHEVILSGLT
                     LRGKFIKRYTAEDKQKVEPIAVKMGIENLLHRAIGELSGGQLQRVLLGRAIIDNPKLI
                     ILDEPSSYVDKLFETNFYKLLDEINKEIAIVLVSHDVGTIISQVKNIACVNQSLHYHC
                     GSNISQEWLMGAYDSCPIEILGHGSLPHRVLLQHDHDHDEKE"
     gene            complement(8183..9073)
                     /locus_tag="PLI30_03515"
     CDS             complement(8183..9073)
                     /locus_tag="PLI30_03515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006799552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HOI78655.1"
                     /translation="MRKYIFTLLFPFLLLACHSNREKEANVLTVTIEPQRYFLEQIVG
                     DKFQVNTLVPPGSSPETYEPSPSAMIKLGNSRAYFKVGFLGYENVWTGKLAENNPDLK
                     IVDCSVGIEPVYGGHGHHDHSHEAADHGHNQTDAADPHVWSSAKNAVLFSQNMLNAVV
                     ELDVENAGLYRANFEKLTQKIAETDSALTRLLKDIPTRSFIVYHPALAYLARDYNLTQ
                     HSIEFEGKNPSPAQMKELVDLAKAENIKIVFVQQEFDIKNSEVIAREIGATSHTINPL
                     AYEWDEELIRIAQLLASQEK"
     gene            9184..9852
                     /gene="mnmD"
                     /locus_tag="PLI30_03520"
     CDS             9184..9852
                     /gene="mnmD"
                     /locus_tag="PLI30_03520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006801442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016645 - oxidoreductase activity, acting
                     on the CH-NH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (5-methylaminomethyl-2-thiouridine)(34)-methyltransferase
                     MnmD"
                     /protein_id="HOI78656.1"
                     /translation="MNIKFEITEDGSHTLFVPERNEHYHSTHGAIQESMHVYIDAGLR
                     HCVKSEVKVLEIGFGTGLNAFLTLLEAEKNSRKIDFTSLELYPVSITDAEKLNYAELI
                     DPTRKEVFNELHKTGWGKWNQVALYFSLLKMRLDFSNPSNFHPENKFDVVFFDAFAPE
                     KQPEMWTPAIFDKIYSVSSENAILTTYCAKGSVRRMLQAAGFFVERLPGPPGKREMLR
                     AVKK"
BASE COUNT         2734 a         2056 c         2294 g         2878 t
ORIGIN      
        1 ttgattgtga ccccggaggg gctcgaaccc tcgacccatt gattaagagt caattgctct
       61 accagctgag ctacggagtc tgaaaaaccg cacaaagtta cggattattt ttcttataaa
      121 aacaaaaata aggagcaaag ttattggact ttgctcctta tttagatcgt ttgactaaat
      181 tattagaggt tggacaaggc tgcgcctgct ttaaatttag ctgctttctt agccgggatg
      241 ttgattgctt ttttcgtgcg gggattaata ccttttcttg cacttctttg tgttaaagaa
      301 aaggttccaa agcctaccaa tacaactttg ccaccttttt tcacttcacc cgagattgca
      361 tcgagggttg cgtccaatgc tttttttgca tcaactttgc tgaggcctga tttttcagca
      421 atagcactaa ctagttctga tttattcata agaaaacgtt aataaaaaat taaacaaaca
      481 ttaatgttaa ggttttcacc tattttagcg ccaaatataa ttgattattt aaaaacaatc
      541 aagaaaaaag ataaaaagta tttgatttta tttaaaaaaa aggaattatg ccccaaaatc
      601 tcgacataat acgaaaatat acgtattttt gcttttcatt tccataaagc aattatgaac
      661 aatttaagat cgggatttgg tgctgcattg ccgccggtga cactgaatat aattataata
      721 aatgttattt tatggctggc tcaggtggtt tttttgaggc agggaatcaa cttggccgaa
      781 ctgttcgggc tgcattatat agcgtccgaa ggcttccggg tttatcagct agtgacatac
      841 atgtttcttc acgatagcgg ttcgtttatg cacgttttct ccaatatgtt tgccgttttt
      901 atgtttgggc gtacattgga gcacgtgtgg ggatcgaaac ggtttcttag tttttacctg
      961 gtaacgggtg ttggagccgg gctggtgcaa ctggttgtcg cttttttacg tattcagtct
     1021 gttaagagcg gtatgtctgt tgaagccata gaggaggttt actcccaggg atatcagctt
     1081 ctgcaacaaa atatgaatta catcaacagt gtacagggta gcttgaacat tgctctgaat
     1141 tcggttacgg taggggcttc cggagctgtt tttgggatac tgctagcttt tgggatgttg
     1201 tttcccaatg cggaactttt tatcattccg tttccgtttc ccatcaaagc aaaatggttt
     1261 gttctcggat acggtgtttt tgaacttatc ttcggtatca gtaacttttc ttgggataat
     1321 gttgcccatt ttgcacacct gggaggtatg ttattcggca tatttatgat attatattgg
     1381 aggaaaaaag acagggtaca tggcaactat tattgatcgg ttaaaataca ggtataaaaa
     1441 cggggatgta ctggtgaagt ttgttttcat caacgcagct gtttttctca tactcaaggt
     1501 ggtcggcatc ttttttgtcc tgttcaatat cagtctggta gacctggttt cttttttggg
     1561 tgttccttcc gaattatctc agctgctaaa ccgcttctgg accctcctta gttatatgtt
     1621 tgtccacgaa caattctggc acctgctttt caatatgctt tggctttatt ggtttggacg
     1681 catttttctg caatacttca gcggaaggac gttaggcagt ttgtacgttt tgggaggttt
     1741 tgccggcgca tttctctata tcgtagcgtt taatacagtt ccctattttg ttggaatggg
     1801 tcacagttgg atgattggag catctgccgc cgtcatggct gtcgtgatgg gtgccgcttt
     1861 ttaccgcccc aatgttcagt taaacctgtt gctgataggt caggtgagga ttgtttatat
     1921 tgctgttggc gttttcttga tagacttcct ctccttgggt aatgggataa acgaaggcgg
     1981 acatgttgcc catatcggcg gagctattgc gggttatatc tttgcgcgac agtaccggaa
     2041 cggacgtgat attaccggtt gggtgagtaa actgcttgac ggtttggcca acctgtcgaa
     2101 gccgagaaga aaaaaaacaa aaatgaaagt tgagtaccgt cgaagagaaa cagataggga
     2161 atataatgag cgcaagcata atgaacaggc agagatcgat gctatcctgg ataaattgaa
     2221 aaagtcggga tatagtaatt tatcgtcgga agagaaaaag aagttgttcg atgccagtaa
     2281 aaaataaaat agccttgttc cggggaatag taagaaacat aatattcgct acgaatatta
     2341 tagccatact gttgcttttc agttcatttt tatcctggcg cgtctcgccg ctcaaaacca
     2401 acctgttctc ctacatcgga atcgcgttcg ggtttgtttt cttcctgaat atcagctatc
     2461 tttttttatg gatagctttt aaaaaatgga aattggcatt cttctctctt gtctctctgc
     2521 tgttgtgtta tcatccgata gctacttttt tcccgatgaa catctttccg gagaaagtcc
     2581 ctaggaacag cttgaaaatc cttacttata acgtggaggg ttttgtcaac gaaaacaaaa
     2641 aagaggacaa agagcatcct atcctcgatt atattgtaga aacggatgct gatattgttt
     2701 gcctgcagga gtacctcgta agtaaaacag ggcaatcgat caaatcgcag cgcgatgtga
     2761 accgtatcct caataaatat ccgtatcact cttctacggc actggaagcg tcggggaaat
     2821 accatattta cgggttggct tgtttctcca aataccccat tgaaaaaacc catgaagtcg
     2881 tttttaattc gtcgtttaac ggtgctgcgg tttataccat tgatgtaaac gggaaaaaac
     2941 ttgctgttgc caatgtacat cttgagtcga acagtatttc tgcagaagat aagaaacttt
     3001 atggcgattt cattcagaac agtgatgagg taaacctgga agatgtaaca tccaatattc
     3061 gttcccgttt agggagagct tatcgcatgc gtgcggaaca ggtacggaaa gtaaaagatc
     3121 atctctcgga attaaatgtt gatggaaccc tgatttgcgg cgattttaat gatacaccca
     3181 tttcttacgt ttacgcacag atgaaggaag gaatggatga cgcgtttgca tccaccggtt
     3241 tcggtccggg gatcacctat cataaggatt tcttctggtt ccggatcgac aatattatgc
     3301 acagccccaa cctgaaatcc tacaaggcga aggtagacaa agtgccctat tccgatcatt
     3361 atccgttaac tacctttttg aacgtgggcg actaattatt cccttggctt tatctgtttc
     3421 gcagaaagcg atgctgagag tctggtgaaa tagaacacta aaaaatgata aaaatttatg
     3481 tataagataa gtaaacagtt tgcattttcc gctgcgcatt cgcttgacgg attgcccggc
     3541 gatcatccgt gtacccgttt acacgggcat aattatgtgg ttacggtgca ccttcggtcc
     3601 aaagagctga acgatacggg atttgtgaaa gactacaacg agttgaaggt cgtaaagcaa
     3661 tatattgatg aacatatcga tcaccgaaac ctgaatgaca tcatgtatcc gttgaactcc
     3721 tcagccgaaa acctggctcg gatgtttttc gatattttta aaccgatgct tcccgagttg
     3781 tatgccgttg aagtgagtga aacaccaaaa acttctgcta tatatgagct tgataattaa
     3841 tgaaatattt tattcattac agggagaagg aggccgtact ggcgaagctt ccgtatttat
     3901 caggttgtcc aaatgcaatt tagcgtgtag tttttgtgat accgatttca agtctgggaa
     3961 gagcatggcg ctcgacgaga tctacgctga aatttcatcc tatccttgcc ggtggattat
     4021 ctggacggga ggtgaaccta cgcttcaact taacgaggag atcgttgctt ttttcaaaga
     4081 caagggatac aggcaagcca ttgaaaccaa tgggacgcgc cgggttcctt cggggatcga
     4141 ctacatcacg tgcagtccca aacagcagtt cgggaagatt cgcatactta ttcctgaggt
     4201 ggatgaattg cgctttccca tggccaaggg cgatcccttg cccgatgtct ctgtcctccc
     4261 caaaacaaga cgctatttgc taagccctat ctttgacggc atgaacgtta tacaggaaaa
     4321 tgtggattac tgcgtggagc ttatcaagca aaatccccac tgggggctga gtttacaagt
     4381 tcataagctt attggaatca ggtaactgta agctatccgt ttgaaatttt atgactcgct
     4441 ttgaaataac gattttaggt tgtggttcgg caatgccaac cacgctgcat aacccttctt
     4501 cgcaactggt caatgtaaac gaaaaactct ttatgatcga ttgcggtgaa ggtacccagc
     4561 ttcagttgcg gaagtataaa acccggataa acaagctgca cagcattttc atatcccatt
     4621 tgcacggcga ccatgttttc gggcttcccg gattgctggc tacactgagt ttattgggaa
     4681 ggaccggcga tttgaatatc tacgcgcaca aggatatgga gttgtttctc gaccctttca
     4741 tcaaacactt cggcaaccag ttcccgtaca aaataaacct cactcctttg gatccttatc
     4801 aacatcaact gatcttcgaa aacaactcca tacgcgtttt ttcgtttcct ttgaagcacc
     4861 ggatacccac cagcggattt ttattcgaag agaaagaccg gccccgtcac atcaagcggg
     4921 agatgattga cttttacaac attcccgtct gcaagataaa tgagatcaaa aaagggggtg
     4981 actacctgac tgatgatgga aaatggatta aaaatgaaat tctcactact ccggccattc
     5041 cacccaggac ctatgcttat tgttcggata ctgcctattc tcccgaaatt attccataca
     5101 ttcaacattc cgatgtgctg tatcacgaag ctacctttgc tgaagccgag aacctgagag
     5161 cgcaggagac gtaccattcc actgcacgac aggcggccga aatcgccagg tcggcaaacg
     5221 tgagaaaact tattatcggt catttttcgt cacggtacca tgaattgggc gaattgcttg
     5281 acgaagccca ggccgttttt cctgaaacag aactggctga tgaagggatg acgattaatc
     5341 tttaagagaa acagttacaa caacgtaatt ttttcctact tttgcagggt attgaacaca
     5401 aaacagtttg tgaggttttc aaccccggag gttcgacact caaaagggtt aagtatgaag
     5461 agattttact ggacaacgct atgtatattg ctttttttat gcggttttgg gattgacgga
     5521 atttacgcac aagatcaaaa caggaaagag gttatggaag aggtcgtccc tgaaacaaaa
     5581 atcaaggtaa cggaaaaccg gctggtcatc gaaaatcttc cgaaagatgg agtgttggaa
     5641 attttcagca tcatgggtgt aaaagtgtat acccggaaaa taaaagccgg aacgaacgaa
     5701 taccaactcg accttcctaa ggggtattac atcatccgga ttggagattt agtcaaaaag
     5761 atattattaa agtgaaaaaa gtatatttta gcgcataaag ttataaaagc acttgctttt
     5821 tgaaggcgat gttgcgtgta atggtagttt ttttatacct ttgttgtttc atttatttat
     5881 catcattaaa acagttacta aaatgaaaat tgcgattgtt ggaacaagcg gagcggtagg
     5941 tcaggaattg cttcgtgtac ttgatgcaag aaatttccct gtggatgaat tgtttctttt
     6001 cggttcttcc agaagtgcag gatcaacata tacttttcgc ggtaaagaaa ttaccgtgaa
     6061 agaactcaag cataacgacg attttaaagg gattgatatt gcttttgtct cggcaggagg
     6121 cggaacatcg ttggaatttg cagaaacgat tacccggtac ggaaccatca tgatagacaa
     6181 ttcaagtgcc ttccggatgg atgacgatgt tccgttagtc gtacccgaag tgaatgcgga
     6241 agatgcgctg aagcgtccca gaaacatcat tgccaatcct aattgtacta ctattcagat
     6301 ggttgtggcg ttgaaagcca tcgaagatat ttcacacatt aagcgcgtac acgtgtctac
     6361 ctaccaggca gcttcgggag cgggcgcctc agccatggcc gagttgcagg aacagcacag
     6421 gcaactggtg aacaacgaaa atccgacgat ctctaaattt gcgtatcaat tggcatacaa
     6481 cctgatacca caggtagacg tgtttactga aaacggatac actaaggaag agatgaagat
     6541 gttcaacgaa actcgcaaga taatgcacag caatatcgaa gtgagtgcta cctgcatacg
     6601 tgttcctgta ttacgtgcac attcggaagc tatctggatc gagacggaaa ggccggtgtc
     6661 ggtggaggaa gctcgtgaag cgtttgccaa agccgaaggc gttgtattac tcgatagccc
     6721 ggagaaaaaa gtatacccga tgccgttgga tgctgccgga caagatccgg tctatgtggg
     6781 aaggattcga aaagacctta ctaaccccaa cggattgacg ttctggacag cagccgatca
     6841 aatctgtaag ggtgccgcct taaatgcggt gcaaattgca gagtatttga tgaaacagta
     6901 aaacagtata ggttttatac caacgtcatc acaaaaaaga aaacgatcaa ccccacggtg
     6961 atcagcttaa taatgagtcc ggcaatgtag ccgaaaaatg cgcctgacgc gtgttttacg
     7021 gcgggtttta ttttattccc gacgattaat ccactcacga gcgctccggc aaaagggccg
     7081 aaaataatcc cccacggacc caggaaaaaa ccgatcagta atccgatggt tgctccccag
     7141 accacttttt tgtttccgcc catcttccgg gttccccaca gcggcaaaat attgtcgagg
     7201 atagtgacgc cgatggtgat aattcccaga agcacgatta cggtccacga aacgttatct
     7261 tttaccgaag gtacgaattt cagcgccagc aatcctgccc aacaaagcgg aacacccggt
     7321 aataccggta caaccgtacc gacggctcct acaataacgc agattacggc cagggagatt
     7381 agtaacggct cgggcaacat gagttattct ttttcatcgt gatcgtggtc atgctgcaaa
     7441 agcacccggt gcggcaggga accgtggcca agtatttcga tgggacacga atcgtaagcc
     7501 cccatgagcc actcctgcga aatattgctt ccacaatggt agtgaaggct ttgattgacg
     7561 cacgcaatat tttttacctg cgaaataatg gttcccacgt cgtgggagac cagcacgata
     7621 gctatctctt tatttatttc gtcgagcaat ttgtaaaaat tagtttcgaa gagtttgtcg
     7681 acatatgagc ttggctcgtc gagaatgata agcttggggt tgtcgataat ggctcgcccc
     7741 agcaatacgc gttgcagttg tccgcccgaa agttcgccga tagccctgtg cagtaggttt
     7801 tcaatgccca tttttacggc aattggttct accttttgtt tgtcttctgc cgtgtatctt
     7861 ttgataaact tcccccgtag cgttaagccg gagagaataa cttcgtgtac gctgatgggg
     7921 aattttttat cgatctggtt tatctgtggc aggtaaccga tgttaaggtg gctaactttt
     7981 tttccggcat cgagaaacgt gatattgccg gaagacggtt gtattaaccc taaaatagct
     8041 ttcagcaggg tggtttttcc tccgccgtta ggcccgataa tacccagaaa atcattttcg
     8101 aaaacggtga gcgaaacgtt tttcagtaca tcgggcttgt tttcgtagcc ggcgttcagg
     8161 ttatgtattt caatgatcgg gttcattttt cctggctggc taacagttgg gcaatgcgaa
     8221 tcaattcttc gtcccattca taagccaatg ggttgatggt atgtgatgtg gccccgattt
     8281 cccgggcaat aacctcgctg ttttttatat cgaattcctg ctgaacgaaa acgatcttga
     8341 tgttttcagc ctttgccaga tccaccagtt ctttcatttg agcgggtgac gggttcttcc
     8401 cttcaaactc aatactgtgc tgcgtcaggt tgtagtcgcg ggcaagatag gccaacgccg
     8461 gatggtaaac gataaaactc cgggtaggaa tatctttcag caagcgggtt aacgcggaat
     8521 cggtttcggc gatcttttgc gtcagttttt cgaagttggc ccggtagaga ccggcgtttt
     8581 caacgtccag ttccactact gcgttcaaca tattttgcga aaaaagaacc gcattcttgg
     8641 cagagctcca tacatgcgga tcagcagcat ctgtctggtt atgaccgtgg tctgcggcct
     8701 cgtgcgaatg atcgtgatgc ccgtgtccgc cataaacagg ttcaattcct actgaacaat
     8761 ccacgatttt caggtcagga ttgttttcgg caagttttcc cgtccacacg ttttcgtaac
     8821 ccagaaaacc tactttaaaa taggcgcgac tgtttcccag ctttatcatg gccgagggcg
     8881 acggttcata ggtttccggg cttgaacccg gagggacaag cgtgtttacc tgaaatttat
     8941 cgcccacgat ttgttccaga aaataccgct gcggctcaat agttaccgtg agtacgtttg
     9001 cttccttttc gcggttggaa tggcaggcaa gaagtaagaa tggaaataag agcgtgaaaa
     9061 tatattttct catcctgttg ggttgattgt tttgttagaa tctgttgcaa aattaagata
     9121 aatagcgcaa caggaaagtt ataaacaaag attttctatt tttgtttgtt gttaaagacg
     9181 gacatgaaca tcaaatttga aattaccgaa gacggttcgc acaccctttt tgttcctgaa
     9241 cggaacgaac attaccattc cacgcatgga gccatacagg aatcgatgca cgtgtatatt
     9301 gatgccggat tacgacattg cgtgaaaagc gaagtaaagg tgctggaaat cggtttcgga
     9361 accgggttaa atgcatttct gaccttgttg gaagccgaaa aaaacagcag gaaaatagat
     9421 ttcacttcac ttgaactcta tcctgtttca ataaccgatg cagaaaagtt aaattatgcc
     9481 gagttgattg atcccacccg aaaggaagtt tttaatgagt tgcacaaaac aggctgggga
     9541 aaatggaatc aggtagcgct ctatttttcg ctgctgaaaa tgcggctcga tttcagcaac
     9601 ccatctaatt tccatccgga gaacaagttt gatgtcgtct ttttcgatgc ttttgctcct
     9661 gaaaaacaac ccgaaatgtg gacaccggca attttcgata aaatatattc tgtttcttcc
     9721 gaaaatgcga tattgaccac ttattgcgcc aaagggtccg tgaggagaat gttacaggct
     9781 gcgggtttct ttgttgagag gctgccgggg ccgcccggaa aaagggaaat gttgcgggca
     9841 gtcaaaaaat aggccgcgtg ctcatttgga tatcacccga acgtttatct tgtgattgaa
     9901 ttgctcttca gtgggtgaaa aaagttcgag taaagttgtc atatcccttg tttcccagat
     9961 at
//
LOCUS       DAOSDR010000055         9946 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrimonas sp. isolate AMR_MDS_1826
            NODE_2567_length_9946_cov_4.429178, whole genome shotgun sequence.
ACCESSION   DAOSDR010000055 DAOSDR010000000
VERSION     DAOSDR010000055.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863355
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrimonas sp. (wastewater metagenome)
  ORGANISM  Petrimonas sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Dysgonomonadaceae; Petrimonas.
REFERENCE   1  (bases 1 to 9946)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9946)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:10:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,720
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 35
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9946
                     /organism="Petrimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2567_length_9946_cov_4.429178"
                     /isolate="AMR_MDS_1826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2023866"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..278)
                     /locus_tag="PLI30_03525"
     CDS             complement(<1..278)
                     /locus_tag="PLI30_03525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009860481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor"
                     /protein_id="HOI78657.1"
                     /translation="MSNTIKNRIEFENIYVAHYSRMKRFAQEYVIREEDAENIVQDVF
                     LDLWEQNFLLLTHTNLFAYLFTTLKNRCIDFLRHKTIVQNTAQKLQDD"
     gene            complement(370..1761)
                     /locus_tag="PLI30_03530"
     CDS             complement(370..1761)
                     /locus_tag="PLI30_03530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019538256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004560 - alpha-L-fucosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-fucosidase"
                     /protein_id="HOI78658.1"
                     /translation="MSVKLKKKGISLLFVLLSFASLSAQYQPHWDSLDKREVPQWWQD
                     AKFGIFIHWGLYSVPAYAPVNEVDGIYEKYAEHYYNRLLTGNKFFQDFHAKQYGEHFK
                     YADFAPLFKAEYFNSDEWAELFREAGAKYVVLTSKHHDGFCLWPSTQSPHWNSVVMGP
                     HTDLIGKLSTSVRNTGLRFGLYYSLLEWAHPLYSQPTIEKWVDRHMIPQMKELVNNYK
                     PEIIFSDGEWDYDSKTLKSEEFLAWLYNESPVKNSVVVNDRWGKETRSKHGGYYTTEY
                     DLVHSQQGIGDNADHPWEESRGIGTSYGYNRFETTEHYLSSKQLIDILIDKVANGGNF
                     LLNIGPDATGMIPVVMQERLLDIGKWLKVNGDAIYFSQPWKSPNRPKNENLLFTRKGG
                     NLYILLKKWPKTPVILTGLKRAGKVELLGYKGTLNAKQQQGKLTITFPPSLTVDELPS
                     QHAWVIKVSDFRE"
     gene            complement(1932..3035)
                     /locus_tag="PLI30_03535"
     CDS             complement(1932..3035)
                     /locus_tag="PLI30_03535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6051 family protein"
                     /protein_id="HOI78659.1"
                     /translation="MNYCQRFTELGNLFKQGADCRLPETKTSIRFFSFGSNLCSGETL
                     PADCHIEENYSFEYPVFTPCGKQKQDKAILLLHGLNERNWTKYLTWAEYLCSNTGKPV
                     ILFPIAFHINRSPLSWSTPRALSSLLNCRRNKYTDDRSISFANVALSERISQNPERFY
                     LSGRQTCADLTSLYEEIKTGKHPLFTEGTHIDIFAYSIGAFLSQVALMANPKGLFADT
                     RLFMFCGGSIFRSMHGVSRSIMDRAAFEKLYNYYVYTFGMEPIAKWFRDKAFDAFFQM
                     ILPERFQTQRESFFERIGEKIRGIVLAQDVVIPYHGVQEALGIKNTEVRIELLDFPYP
                     YSHENPFPVNLKDVSSVDRSFMNVFSQAAGFLE"
     gene            3234..4091
                     /locus_tag="PLI30_03540"
     CDS             3234..4091
                     /locus_tag="PLI30_03540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019540049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HOI78660.1"
                     /translation="MNGKIPAYLYEKGNTVKMIVFTAVFALLFINIFQPFGSRAWYPA
                     ISDFKYFTFSSLIILTGMLVVVVSRVILAVYSKKNDILYWQYGFWILCEVLAMAVFYS
                     LFSKFVPKEGADRDFMDIFRQSAVNTALVLLLPYAISWLYFSWKEKSLLLANMEQEKT
                     ADSHTRTLVGFPDEKGDLKISITLENLLYIESADNYATIFYLNKSRQSRFLLRNSLKW
                     MEENLTKDTPLVRCHRSYIVNMDKVKILKKVKGGIVLELDAENTPELPVSKTYYESFM
                     HKFSRYSVN"
     gene            4307..4688
                     /gene="rnpB"
                     /locus_tag="PLI30_03545"
     ncRNA           4307..4688
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="PLI30_03545"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            4701..6248
                     /locus_tag="PLI30_03550"
     CDS             4701..6248
                     /locus_tag="PLI30_03550"
                     /inference="COORDINATES: protein motif:HMM:TIGR00765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HOI78661.1"
                     /translation="MLKRKKRVSKQEEEDKPGLFERIRLQIKEWVHFLTYEIWRLNPE
                     NFSNKKNVLHNVLKVTMLTVRGVQEQNLGASSRSLTYRTILSLVPMLAVLFAIARGFG
                     FEKILESQIFDFFSNSDTEITAALPESKMATDTLIDVSESIGVNQEINIVSTVQKPDS
                     LDVDSSITETTSFTIETIIKLVNNSLEHAKGGVFAGIGVILLLYTVILLFSDIENSFN
                     RIWGVKKGRSIQRRAVDYFALILLLPIFAVLNYSLTALIQASTGPFDKFSYILDPVVT
                     QVLNFLPFLVMIITLTLLYRFMPNTKVKWASALIGGIVGGVAIQLFQMIYLNGQLWIA
                     KYNAIYGAFAAIPLLLLWVQMSWFIVLIGAELSYSAQNVRKFSFDRETKNISRRFRDF
                     FTIMIASVIVRRFAEGETPLTADQISMKCKIPIKLTNDIIDELHKLQVILPTPTPDDD
                     RVMAYQPALDINLLTVNDLIEQIDKEGSEDFLIDVEGDFARHWNALMHTRMSVYESPS
                     DIMLKDL"
     gene            complement(6250..6963)
                     /locus_tag="PLI30_03555"
     CDS             complement(6250..6963)
                     /locus_tag="PLI30_03555"
                     /inference="COORDINATES: protein motif:HMM:NF013980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF45 domain-containing protein"
                     /protein_id="HOI78662.1"
                     /translation="MYEYNDKELGKIIVKPDTRAKRIIARRKGEYIQLTVPFGFTPKR
                     LPSLLDDMRHRLTKLFTARDQLITEDSVLETFSFTARLSRTHLVENVKMRLKDGNLTI
                     FVPGNFDLENSTSQHIVKEMIRQALRHEAKRILPEKTRKFAQVLHLQVNDIKINKSVS
                     RWGSCSRRKTINFSYYTMLLSEKQIDYVILHELAHTVELNHSEKFWNLLNRFCGEDAK
                     TISRSLRKYPSKAYQLLRP"
     gene            7142..7885
                     /locus_tag="PLI30_03560"
     CDS             7142..7885
                     /locus_tag="PLI30_03560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009122825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOI78663.1"
                     /translation="MISSLESVGEYVELMRRVIAPPDRAREFFKEFSKEVYKLGVNSI
                     WLVIIISFFIGAVIVMQIAINVASPLLPRFTVGVVSREIILLEFSSTIMCLILSGKVG
                     SNIASEIGTMRVTEQIDALDIMGVNSANYLIMPKVSAFVFFMPVLVGLSMFLGMFGGY
                     VICLFTGTPPVSTYIYGIQFFFRESFVWTSILKSMIYGFIIASVSAYFGYHVKGGSLE
                     VGKASTDSVVINNILILAADLVFTQLVMG"
     gene            7905..8699
                     /locus_tag="PLI30_03565"
     CDS             7905..8699
                     /locus_tag="PLI30_03565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006844098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOI78664.1"
                     /translation="MIQVRNLIKEFEGRLVLDDITTSFKKGAVNMIIGKSGSGKTVFL
                     KCLIGLIQPSSGEIRYNGRDITEMRAREMKRLRRDMGMLFQGSALFDSKTVLENVMFP
                     LDMFSRMNLRERKKRAEFCLERVNLSHAADLYPSEISGGMMKRAAIARAIALNPRYLF
                     CDEPNSGLDPKTAQLIDELISDITEDFGITSIVVSHDMNSVMNIGDNIIFLNEGILEW
                     QGSKDKIMDANNEKLNEFVFASELFKKIKAADTIITENNASGQEAG"
     gene            8788..9540
                     /gene="sufC"
                     /locus_tag="PLI30_03570"
     CDS             8788..9540
                     /gene="sufC"
                     /locus_tag="PLI30_03570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006844096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HOI78665.1"
                     /translation="MTNLLRIKNLHAVVENKEILKGIHLEVNEGEIHAIMGPNGSGKS
                     TLASVLAGNPKYEVTRGSVIFKEKELLDMEPADRAKEGIFLSFQYPIEIPGVSMVNFM
                     RAALNEQRKYRGEEQISASNFLKLMRDKRELLGIDSQLVSRSVNEGFSGGEKKRNEIF
                     QMAMLDPTLAILDETDSGLDIDALRIVAAGVNALHSPEKAAIVITHYQRLLDYIQPDV
                     VHVLYDGKIIKTGGPDLALELEEKGYDWLVNP"
     gene            9553..>9946
                     /locus_tag="PLI30_03575"
     CDS             9553..>9946
                     /locus_tag="PLI30_03575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019540044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HOI78666.1"
                     /translation="MIEQQYIDLFNDYRDEIDANSVAGLNRHRDAAFEAFNRSGFPTS
                     KSEDYKHTDVSRVFDCDLGLNLRNVPVPVDPYEAFKCDVPNLNTNVYFLINDRFYTNG
                     HHQGPLPKGVFVGSLQLFSETHPDIFERY"
BASE COUNT         2721 a         2065 c         2293 g         2867 t
ORIGIN      
        1 tagtcgtcct gtaatttttg tgctgtattc tgcacaattg tcttgtgcct taaaaaatcg
       61 atacacctgt tctttagcgt cgtaaacaga taagcgaaaa gattggtgtg cgtcagcaaa
      121 agaaagttct gctcccacaa atcgagaaag acatcctgca caatattctc tgcatcctct
      181 tcgcgaataa cgtattcctg tgcaaaacgt ttcatcctcg agtagtgggc aacatagata
      241 ttctcgaatt caatcctgtt tttaatggta ttgctcattc ttgtctgcta aagctcaaaa
      301 tgaaattaca aactcaaaaa accaaaaaaa caacataaag gaagtgaaaa ccttcccaaa
      361 atagaaacac tactccctga aatctgaaac tttaattacc cacgcatgtt gggaaggtag
      421 ttcatcaaca gttaaggagg gggggaaggt gatcgtcagt tttccctgtt gttgttttgc
      481 attcagggtt cccttgtatc ccaataattc aactttcccc gcacgtttca ggccggtaag
      541 gatgacaggt gtctttggcc acttctttaa aaggatatac aaattccctc ctttccgggt
      601 gaagagcagg ttttcgtttt ttggcctgtt gggacttttc cacggctgcg aaaaatagat
      661 ggcatctccg ttcactttca gccatttccc tatatccaat aacctttcct gcataacgac
      721 agggatcatg ccggttgcat cggggcctat gttcagtagg aaattaccgc cattcgccac
      781 tttatcgata aggatatcga tcagctgttt tgaagaaagg taatgttccg ttgtttcgaa
      841 acggttgtat ccgtaggatg ttcctatacc gcggctttct tcccacggat gatccgcgtt
      901 gtctccaata ccctgctgac tgtgcaccag atcgtattcc gtggtatagt agcccccgtg
      961 tttgcttctg gtctcctttc cccacctgtc gtttactact accgagtttt ttacgggaga
     1021 ttcgttgtac aaccaggcta gaaactcttc gcttttcaag gttttgctgt cgtaatccca
     1081 ttctccatcg gagaaaatga tttcgggctt gtaattgttg accagctcct tcatctgcgg
     1141 aatcatgtgc ctgtccaccc atttttcaat ggtgggctgg gagtacaacg gatgagccca
     1201 ttccaacagg gaataataca acccgaaacg gagaccggta ttgcggactg aggtggataa
     1261 cttaccgatt aaatcggtat gcggccccat caccacgctg ttccaatgag gggattgtgt
     1321 actgggccat aaacaaaaac catcgtggtg cttggatgtc agcacgacgt atttcgcacc
     1381 cgcttccctg aaaagttctg cccactcgtc ggaattaaaa tattcggctt taaagagggg
     1441 tgcaaaatca gcgtatttga agtgttcacc gtattgtttt gcgtgaaaat cctgaaaaaa
     1501 cttgttgccg gttaagagcc ggttgtaata atgttcggca tacttttcgt aaataccatc
     1561 tacttcattg acaggagcgt aggcgggaac ggaatacagt ccccagtgaa taaaaatacc
     1621 gaacttggca tcctgccacc attggggtac ttccctttta tccaacgaat cccagtgggg
     1681 ttggtattga gcagacagtg atgcaaagga aagtaatacg aatagcaggc tgatcccttt
     1741 tttcttcaat tttaccgaca taattagttc tatttgaaat tttactaaat ataataatct
     1801 cccaaccgat tttgatggag attccgaaga ctggagcaat tccgggaatt actcattcct
     1861 caaactttcg agcccaaata tacaaataaa acattaaaat cagcttgtaa agaaaaaaaa
     1921 agttaaaaag cctattctaa aaaaccggct gcctgggaaa agacattcat aaaagaacgg
     1981 tcaacggatg aaacatcttt gaggttcacg ggaaaaggat tttcgtggga ataaggatat
     2041 ggaaaatcca gtaattcaat tcttacttcc gtattcttta tccccagcgc ttcttgtact
     2101 ccatggtatg gaattaccac atcttgcgca agaacaatgc ctctgatttt ttccccgatc
     2161 cgttcaaaaa aactttcgcg ctgtgtttga aacctctcgg gtaaaatcat ctggaaaaat
     2221 gcgtcgaaag ctttgtcccg aaaccattta gcaattggtt ccatgccgaa agtataaacg
     2281 tagtagttat acagcttttc aaaggcggcc ctatccataa tgcttcggga aacgccatgc
     2341 atggaacgga atatactccc tccacaaaac ataaacaggc gcgtatcggc gaagagccct
     2401 tttgggtttg ccatgagtgc cacctgcgaa aggaatgctc ctatggaata ggcaaaaatg
     2461 tcgatatgcg tgccttccgt gaaaagggga tgttttcctg tttttatttc ttcgtataaa
     2521 gaagtcaagt cggcacaggt ttgcctgccc gataaataaa agcgttccgg gttctggctg
     2581 atgcgttcgc tcaacgctac gttggcaaaa cttatggagc ggtcgtctgt gtatttattt
     2641 cgtctgcagt tcaacaggga agacaaggca cgcggagttg accacgacag gggcgagcgg
     2701 tttatgtgga acgcgatagg aaacaggatg acgggttttc ccgtgttgct gcacaggtac
     2761 tcggcccagg tgaggtattt ggtccagttc ctttcgttca acccgtgcaa aagcaagata
     2821 gctttatcct gtttttgttt tccgcagggg gtaaatacgg gatattcaaa ggaataattt
     2881 tcctcaatgt ggcagtcggc gggtaatgtt tcgccagagc ataagttgct tccgaacgaa
     2941 aaaaaacgga tgctcgtttt tgtttcgggt aagcggcagt cggccccttg tttaaaaaga
     3001 tttcccagct cggtaaatcg ttggcaataa ttcatttttt cttgtaaaga aatatccttt
     3061 ttcaggtatc cgcaaatcaa agggaaaaag gatgcctgca tgtggcagga gcacctatgt
     3121 ggggtggaaa tcgctatagc ggggtgaaat tttttagggc agcgtgcaat tcatttggtt
     3181 aatccttaat aaaaaactat ttttgcatcg ggtcggtaac tgacgctatt aacatgaatg
     3241 ggaaaatacc cgcatactta tacgagaaag gcaacacggt aaaaatgatt gtgttcacag
     3301 cggtttttgc attgctgttc atcaatatct tccaaccttt cggctcaagg gcatggtatc
     3361 ccgctatttc cgattttaag tattttactt tttccagcct gatcattctt accggaatgt
     3421 tggtggtggt tgtcagccgt gttattcttg cggtctattc gaaaaaaaac gatatccttt
     3481 attggcaata tggtttctgg atcttgtgtg aggtactggc catggcggtg ttttattcgc
     3541 ttttttcgaa atttgtgccc aaggagggtg ccgacaggga ttttatggat atctttcgtc
     3601 aaagtgctgt aaacacagcc ttggtgctgt tgttgcccta tgctatttcg tggctttact
     3661 tctcgtggaa ggaaaaaagt ttgcttttag caaacatgga acaggaaaaa acggcggact
     3721 cgcatacaag aaccctggta gggtttcccg acgaaaaggg tgacctgaag atttcgatca
     3781 ccctggaaaa cttgctctat attgaatccg ctgataatta cgccaccatt ttttatttga
     3841 acaaatccag gcaatcccgc ttcctgcttc gcaattcgct aaaatggatg gaggagaatc
     3901 tgacaaaaga tactccgtta gtacgttgtc accgttctta tattgtgaac atggacaagg
     3961 tgaagatact gaaaaaagta aaaggaggga ttgtgcttga actggatgcc gaaaacactc
     4021 ccgagctccc tgtatcaaaa acgtattacg agtcgtttat gcacaagttt tcacggtatt
     4081 cggttaacta aaccttttgt tggtgtgtca ttcacaatca cctttccggt gataatcacc
     4141 ttttcctgta caaaaagcca ccttacgatg cctgttgtaa aggacggtgc gacctggacg
     4201 gctaattgta gccggtagat gtacacaatt cgtaatatgc ttcgtgagtt gtaaaaaatc
     4261 aggtataatt acgctaaatc gttaatttag tattactttt gcagtcgcag caggccggtt
     4321 tgtcgcttcg gtcccgatat ttgtcgggat tcggggagga aagtccgggc aacgtagagc
     4381 accatacttc ctaacgggaa gctgttcgtg agggcagagt agcgtaacag aaaacaaccg
     4441 cttcgttttc tcgtgagatt acgatgcaag ggtgaaaagg taaggtaaga gcttaccggg
     4501 tgccgtggca acacggcatg ccgtacgtct tatgggttgc aagaccatgt atatcggata
     4561 ggtagggttg ctcgcccgat gccgaggggt aggtcgctaa agagcgatgg tgacatcgtc
     4621 tcgcagataa atgacaaaca tcccgacttg tcgggataca gaacccggct tacaggcccg
     4681 ctgctttttt attttgaggt atgctgaaaa ggaaaaaaag agtttcgaaa caggaggagg
     4741 aagacaagcc tgggttgttt gaaaggatca ggctccaaat aaaggaatgg gttcactttc
     4801 ttacttacga aatctggcgg ctaaatcccg aaaatttctc caataaaaag aatgttttgc
     4861 acaatgttct taaagtcacc atgctcaccg ttagaggtgt gcaggagcaa aacctcggag
     4921 ccagttcccg gtcgttgaca taccgtacaa tcctttctct tgtccccatg ttggccgtat
     4981 tgtttgccat tgcccgcggt ttcgggttcg agaaaatcct ggaaagccag attttcgact
     5041 tcttcagcaa cagcgatacg gaaataacgg ctgctttgcc tgagtcaaaa atggcgaccg
     5101 atacccttat cgatgtgtcg gaatccatag gtgtcaatca ggaaataaat attgtgagta
     5161 cggtccaaaa gccggactct ttagacgtcg acagttcgat tacggagacg acctccttca
     5221 ccattgagac aatcatcaag ctggttaaca actccctgga gcatgctaag ggaggggttt
     5281 ttgccggaat cggggttatc ttgctgttat ataccgtcat cttactgttt tccgatatag
     5341 agaacagctt taaccgtatt tggggagtga aaaaaggacg atctatccaa cggagggcgg
     5401 tcgactactt cgcgctcatt ctactattgc ctatttttgc tgtccttaat tacagtctga
     5461 cagctcttat acaagcatct acgggcccgt ttgataagtt cagttatatc cttgaccctg
     5521 tggtaaccca ggtgttgaat tttcttcctt ttttagtaat gatcatcacc ttgacacttc
     5581 tgtacaggtt tatgcccaat acaaaagtga aatgggcaag tgcattgatc ggagggattg
     5641 tcggcggtgt tgctattcag ctttttcaga tgatctattt aaacgggcag ctgtggatag
     5701 caaaatacaa tgccatttac ggggcttttg ccgccatacc gctgttgctt ttgtgggtac
     5761 agatgtcttg gtttattgta ctaataggtg cagagttgtc gtattcggca caaaatgtcc
     5821 gaaaattttc tttcgatagg gaaactaaaa acatcagccg ccgttttcgg gatttcttta
     5881 ccatcatgat tgcatcggta attgtaaggc ggttcgcaga aggtgaaacc ccgcttaccg
     5941 ccgaccagat atcgatgaag tgcaaaatac ccattaagtt aaccaacgat attattgatg
     6001 aactgcacaa gcttcaggtg attttaccga cccccacacc ggatgatgac cgcgttatgg
     6061 cctaccaacc cgctttggat attaatttgc tgactgttaa tgacctgata gagcagatag
     6121 acaaggaggg ctcagaagat tttctgattg atgtagaagg ggatttcgct cggcactgga
     6181 atgccttgat gcatacacga atgtctgttt atgaatctcc cagtgacata atgctgaaag
     6241 atttataaat caaggcctca acagttgata ggcttttgat gggtactttc tgagcgaccg
     6301 gctgatggtt ttggcgtcct caccgcaaaa acgattgagc aagttccaga atttttcact
     6361 gtgattcagt tcgaccgtat gagccagctc atgaagaatc acataatcaa tctgtttttc
     6421 ggatagcaac atcgtgtaat aagagaaatt gatggttttc cggcgggaac aactccccca
     6481 gcgcgacaca cttttgttga tttttatatc gtttacctgc aggtggagta cttgtgcaaa
     6541 ttttcgtgtt ttttccggta aaattctctt ggcttcgtgc cgcaaagcct gcctgatcat
     6601 ttctttaaca atatgttgag aagtggagtt ctccaaatca aaattcccgg ggacaaaaat
     6661 ggttaaattg ccatctttca acctcatttt cacattttct accaggtgtg tcctcgacag
     6721 ccttgcggta aacgagaacg tctctaatac ggagtcttcc gtaatgagtt gatctctagc
     6781 agtaaacagc tttgttaacc gatgacgcat atcgtcaagc agggagggta acctttttgg
     6841 ggtaaatccg aacggaacgg tcaactggat atattcccct tttcttcggg cgataatcct
     6901 ctttgcccga gtgtcgggtt tcacgattat ctttcccagt tctttatcgt tgtattcgta
     6961 catttaacaa agataatgca attatttctc ccaattgttt taccccgcca atcatcaaaa
     7021 taaaaaacat tgcctatctt tgtgcacgtt aggaattgta aacaaataaa ctattcgttc
     7081 tggcttgtga actgttgcat acttatttgt ttacaattcc ttaatttccg atgaaatgag
     7141 cattataagt tctttagaga gtgtcggcga gtatgtcgag ctgatgagaa gagtgatagc
     7201 tccgcccgac cgagctcgag aattttttaa agaattctcg aaggaagtat ataaactggg
     7261 ggtcaactcc atttggctgg ttataattat ctccttcttt ataggggcgg ttattgttat
     7321 gcagattgct ataaacgtag cttctccttt actgccccgc ttcacggttg gagtggtttc
     7381 gcgcgaaatt attttgttgg aattttcttc caccatcatg tgtttgatcc tttcgggaaa
     7441 ggtgggctct aatattgctt cggaaattgg tacgatgcgt gttacagaac agattgatgc
     7501 attggatatt atgggtgtga attcggcgaa ttacctgatt atgcccaaag tttcggcgtt
     7561 tgtgtttttt atgcctgtgt tggtgggatt gagtatgttt ctgggtatgt ttggcgggta
     7621 cgtcatctgt cttttcacag gtactccccc ggtgtccacg tatatatacg ggattcagtt
     7681 ttttttccgg gagagttttg tctggacttc catcctgaaa tcgatgattt acgggtttat
     7741 aatcgcttcg gtttccgcat actttggtta tcacgttaaa ggcggatcac ttgaggtggg
     7801 aaaagcaagt accgactcgg ttgtgatcaa caatatcctg atactggctg ccgatttggt
     7861 tttcacgcaa ttggtcatgg gttgaaaaag ataagtcttg atatatgatt caggtaagaa
     7921 atttaataaa agaattcgaa gggcgtctcg ttctcgacga tattacgact tcctttaaaa
     7981 aaggagccgt gaatatgatc atcggaaaga gcggttcggg caaaacggtt tttttgaaat
     8041 gtcttatcgg cttgatacag cccagttctg gtgagatcag gtataacggt cgtgacatta
     8101 ccgagatgcg agcaagagaa atgaaacgtt tgcgccgtga catggggatg ctgtttcagg
     8161 gatcggccct gttcgactcc aagactgtgc ttgaaaatgt gatgttcccg ttggacatgt
     8221 tttcccggat gaaccttcgt gaaagaaaaa aacgtgccga attctgtctg gaaagggtaa
     8281 acctctcaca tgcggcagat ctttatccgt cggaaatatc gggcggtatg atgaagcggg
     8341 ctgctattgc ccgtgccatt gcgttgaatc ccaggtacct gttttgcgac gaaccaaact
     8401 ccggactcga cccgaaaaca gcacagctta ttgacgaact tatttcggat ataacggaag
     8461 attttgggat tacttctatt gtggtctcac acgatatgaa ctcggtaatg aacatcggcg
     8521 ataatattat cttcctgaac gagggaatcc tggagtggca gggatcgaaa gacaaaataa
     8581 tggacgccaa caatgaaaag ctgaacgaat ttgtttttgc atctgaactt tttaaaaaga
     8641 taaaagcggc cgacaccatt atcacagaaa acaatgcttc gggacaagag gctggctaga
     8701 acaaaacaat tgatgaaaaa aattgttatt cacatagaac atgtgcatag aaatagaaac
     8761 cacaagtaag aataagtagc gagtaatatg actaatttat tgagaataaa aaatctacat
     8821 gcagtagtag agaataaaga aatcctgaaa ggtattcatc tggaagtaaa cgaaggcgaa
     8881 attcatgcta ttatgggccc taacgggtcg ggaaaaagta cgttggcttc tgtacttgcc
     8941 ggaaatccaa aatatgaagt gacacgcggt agcgtaattt tcaaagaaaa ggaactgctc
     9001 gatatggagc ctgccgaccg ggccaaggag ggaattttcc ttagctttca gtaccccatt
     9061 gaaattccgg gtgtaagcat ggttaatttt atgcgtgctg cgttaaatga acaacgtaaa
     9121 tacaggggcg aagagcaaat atcggcatca aacttcttga aactgatgcg tgacaagcgc
     9181 gaattactgg gtatcgacag tcaactggta agccgttcgg tgaacgaggg tttctcggga
     9241 ggggagaaga agaggaacga gatctttcag atggccatgc tcgatcctac gttggctata
     9301 cttgacgaaa ccgattcggg attggatatc gatgcattac gaattgttgc tgccggggta
     9361 aatgcgttgc acagtcccga aaaagctgcc attgtaatta cccattatca gcgacttctc
     9421 gattatattc aacctgatgt ggttcacgtg ctttacgacg gaaaaatcat taaaaccgga
     9481 gggccggatc tggctctcga acttgaagaa aaaggatatg actggcttgt gaacccctaa
     9541 cccgtaacaa aaatgattga acaacaatat atcgatttat tcaacgacta cagagacgag
     9601 atcgatgcga attccgttgc cggactcaat cgtcatcgcg acgctgcttt tgaagcgttc
     9661 aatagatcgg gattccccac ctcaaaatca gaagattata aacatacgga tgtgtcccgc
     9721 gtgttcgact gtgatttagg gttgaacctg cggaacgtcc ctgttcctgt tgatccgtac
     9781 gaagcattca aatgtgacgt gcctaacctc aacaccaatg tatatttcct gataaacgac
     9841 cggttttaca ccaacggaca tcatcaggga cccctgccga aaggagtttt tgtgggtagt
     9901 ttacagctgt tttcagaaac tcatcccgat attttcgaaa ggtatt
//
LOCUS       DAOSDR010000056         9945 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrimonas sp. isolate AMR_MDS_1826
            NODE_2568_length_9945_cov_3.653387, whole genome shotgun sequence.
ACCESSION   DAOSDR010000056 DAOSDR010000000
VERSION     DAOSDR010000056.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863355
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrimonas sp. (wastewater metagenome)
  ORGANISM  Petrimonas sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Dysgonomonadaceae; Petrimonas.
REFERENCE   1  (bases 1 to 9945)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9945)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:10:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,720
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 35
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9945
                     /organism="Petrimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2568_length_9945_cov_3.653387"
                     /isolate="AMR_MDS_1826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2023866"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1673)
                     /gene="yidC"
                     /locus_tag="PLI30_03580"
     CDS             complement(<1..1673)
                     /gene="yidC"
                     /locus_tag="PLI30_03580"
                     /inference="COORDINATES: protein motif:HMM:NF002356.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032977 - membrane insertase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HOI78667.1"
                     /translation="MDKNTIIGFLLIGAIVIAFTFLNRPSQEQIAEQQRLRDSIQQVE
                     TQKAALEAERLATDDPRQTSEIKQQSRVTDFFATGTPAVNDSIGAPTDSTTEIKAAVE
                     EFVTLENEKLKLVLSTLGGSIHSVQLKGEKKFNGDSLYLFEGDEARFNLELFNRNSVR
                     LSTEDQWFTPIPTADGKSVVMRLESSPEQHIDLIYTLPANEFMLDFDVRIAGMKNGLH
                     PESLTNFRVNWEQKVRQQEQGRQFENRFARLNYRYLGQDVQKLSDSKNERKELTEPVK
                     WFAYKDQFLSTIIIAEKPFSNTILTSQPLSTTEHIKDYKAETWVPVTADPQKDELTAS
                     FNYYFGPNHFYTLKNYDKAIKENNQKLYLEELVDLGYKWLSWINKYFTIPVFNYFLKF
                     DWGMGIIILIMTLLIKLIILPLTYKSFKSSAKMRVLRPQIKEIEAKYPGQDKEMMLKR
                     QQATMELYNKAGASPMSGCFPMLLQMPILLAFFFFFPSAIELRQQSFLWAHDLSTFDS
                     IIRWEANIPFISKFLGNHISLFCLLMTVVNVFYTKYNMAATDTGQTQMPG"
     gene            2049..2615
                     /locus_tag="PLI30_03585"
     CDS             2049..2615
                     /locus_tag="PLI30_03585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HOI78668.1"
                     /translation="MNPVTEKIKMVCAGKNIPAEELAANAGLTVEQIELIFNSEKIPS
                     LSSLIKISRALGVRLGTFLDDNENMGPVVNKNSRQSPPVTFTSHLTNANSHIDFFSLA
                     ASKSGRHMEPFLIDIKPSYSSKMTTSSHEGEEFLYVLKGSITVLYGSDKFVLNTGESI
                     YYDSVVEHLVISASDEPAQVLAVVYTPN"
     gene            2627..4276
                     /locus_tag="PLI30_03590"
     CDS             2627..4276
                     /locus_tag="PLI30_03590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011108477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HOI78669.1"
                     /translation="MQLFEKTLGDWLEYWANETPDKEYIVYSDRNLRFTWKSFDERVN
                     NMANGLLSIGVTRGTNVGIWAQNVPDWLTVLYATAKIGAVAVTVNTNYKTEELAFVLK
                     NADMHTLCLTEGIPGSDYIDMIYELLPELRTTQRGKLKNDRFPGMRNVVYIGQEKFRG
                     MYNTAELLLVGASRPKDEFRALREQTGCHDIVNMQYTSGTTGFPKGVMLSHHNITNNG
                     YLTGVHMKFTPDDKCCICVPLFHCFGVVLATMCCLTHGSTQVMVERFDPLLVLASIHK
                     ERCTVLHGVPTMFIAELNHPMFPLFDMSSLRTGIMAGSLCPVELMKQVNEKMFMDITS
                     VYGLTETSPGMTQTHVDDPFGVRCSTVGRAYEFTDVKVLDPVTGRECPVGVEGEMCCR
                     GYNVMKGYYKNPEATAQVIDANGYLHSGDLGMKDEDGNYRITGRIKDMIIRGGENISP
                     KEVEDFLYKMPEVRDVQVVAVASPRYGEDVGAFIILKEGKELTREDVRDFCKDHIARY
                     KIPRYVFFISQFPMTGSGKIQKFRLRQMALEMCEAQGIEII"
     gene            complement(4283..5299)
                     /locus_tag="PLI30_03595"
     CDS             complement(4283..5299)
                     /locus_tag="PLI30_03595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009037726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HOI78670.1"
                     /translation="MIFRKIKHLLIFLFSLSVVSGCEDSMRFDNDPRSNFDALWKILD
                     ENYCFFEYKVIDWDAVYKKYGSRVKNDMGNYALFNLMGEMLSELKDGHVNLIASHDMS
                     RYWSWTEDFPLNFDPNIQKKYLERDFAIAGGMYYKILDDNIGYVYYGSFSNDIGDANM
                     SQVLSQMAVCKGIIFDVRNNGGGSLTNVERIASRFFNEKTLVGYISHKIGKGHSDFSD
                     FYPKYVNSYDKIRYQKPVIVLTNRSCYSATNDFVNVMKHAPNATIIGDKTGGGSGLPF
                     SSELPNGWSIRFSASPMYNVNKEHIEFGIEPDVYVSMTESDKSKGKDSIIEKARELIH
                     SSGR"
     gene            complement(5304..6107)
                     /locus_tag="PLI30_03600"
     CDS             complement(5304..6107)
                     /locus_tag="PLI30_03600"
                     /inference="COORDINATES: protein motif:HMM:NF013357.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003796 - lysozyme activity [Evidence
                     IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA];
                     GO_process: GO:0016998 - cell wall macromolecule catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH25 family lysozyme"
                     /protein_id="HOI78671.1"
                     /translation="MAKRRRAAYSRGKRLKLSKNGRQVVWLIVVFLAALFAVGVYRFI
                     RFSTNEIFHSFDHPQFSQKYLVRGVDVSHHNSYINWHQLREENITFVYLKSTEGSKHK
                     DRDYKKNYKVAKDAGLRIGSYHFYTFGRDGKKQAQHFIRNSTLQSGDMIPAIDVEHSA
                     INRACNDKESHGRMIKELRNLESALFKQYGKRPVIYTNKECYKMYIKDNFPDNPLWIC
                     DLHKEPGGDYKNWVMWQFSHTGKISGVVNDIDLNYYRNSFDDFKKLLLP"
     gene            complement(6133..6588)
                     /locus_tag="PLI30_03605"
     CDS             complement(6133..6588)
                     /locus_tag="PLI30_03605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009317756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RpiB/LacA/LacB family sugar-phosphate isomerase"
                     /protein_id="HOI78672.1"
                     /translation="MSLFDKTEKPIGLASDHAGFAAKQIVIKLLTEKGIPFKDFGAYS
                     EESSDYADFAHPLATAVENGECYPGIAACGSGNGINMTMNKHRDIRSALCWNKEVAYL
                     ARAHNNANVLALPGRLLNEEEIYEILVTFLNTPFEGGRHERRINKIPCE"
     gene            complement(6616..8640)
                     /locus_tag="PLI30_03610"
     CDS             complement(6616..8640)
                     /locus_tag="PLI30_03610"
                     /EC_number="2.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009017147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016744 - transketolase or transaldolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HOI78673.1"
                     /translation="MNDNALMNKAADNIRILSASMVEKAKSGHPGGPMGGADFINVLY
                     SEFLEYDPENPYWEGRDRFFLDAGHMSPMFYSVLCLSGKFSIDETKAFRQWGSPTPGH
                     PERDIERGIENTSGPLGQGHAYGAGAAVAAKFLKARLGDAIDHTIYAYITDGGIQEEI
                     SQGVGRLAGHLGLDNFIMFYDSNNIQLSTTTDAVTSEDVAKKYEAWNWKVITIDGNNV
                     DEIRKALTEAKAEKERPTLIIGNTIMGRGALAADCTSFECQVSTHGQPLSAAGADFAQ
                     TVKNLGGDPENPFVIFPEVTALYDKRKKELNDIVAAKKARYNIWAKANPELAAKKHRW
                     FARELPEIDWASIGQKPDNATRAASATVLGVLAQKVENMIVASADLSNSDKTDGFLKY
                     TKAFEKNDFSGAFLQAGVAEFTLSAMCIGMSLHGGVIPACGTFFAFSDYMKPAIRVAA
                     LMGTPVIFIWTHDAFRVGEDGPTHQPVEQEAQIRLLEKLQNHHGQNSMLVLRPADVHE
                     TTVAWKMALENAQTPTGLILSRQNIKDLPAAGSRYKEALQAEKGAYVVQDENDYDVIL
                     LASGSEVSTLAEGAELLKADGIKTRIVSVPSEGLFRSQTKEYQESILPEGKKKFGLTA
                     GLPVTLEGLVGADGKVWGMNSFGFSAPYKILDEKLGYTGKNVYEQVKKLL"
     gene            8828..9580
                     /locus_tag="PLI30_03615"
     CDS             8828..9580
                     /locus_tag="PLI30_03615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007218476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HOI78674.1"
                     /translation="MPLSKNRIKQIRSLSEKKYRSEHGTFVAEGKKLVLDLLGNCRCQ
                     FLAGLPDILQEIPRLSAEEMVEATPEELKKASHLKTPPQLIGIFYQPKNALEEIEFVG
                     KLHLVLDGIQDPGNMGTIVRLSDWFGIQHVFCSPDTADIFNPKTVQATMGAIARVNVH
                     YVDLKDLLTRNNSLPVFGTLLEGENIYKAELPKNGFIVMGNEGKGISPDIQKLITHKL
                     FIPNYPTNSQTSESLNVAVAAAIVCSEFRRRV"
     gene            complement(9591..>9945)
                     /locus_tag="PLI30_03620"
     CDS             complement(9591..>9945)
                     /locus_tag="PLI30_03620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009016854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="carbamoyl phosphate synthase large subunit"
                     /protein_id="HOI78675.1"
                     /translation="LLEACKLLHAKGYDLYARHGTQQFLTDNGVAATDVNWPDEGGEH
                     NIQEMITHNKFDLIINIPKDVTRRELTNGYIIRRTAVDFNVPLITNARLASAFITAFC
                     MLDVEQLKIKSWQEY"
BASE COUNT         2692 a         2157 c         2177 g         2919 t
ORIGIN      
        1 cccggcattt gggtctgccc ggtatcggtg gccgccatgt tgtatttggt gtaaaacacg
       61 ttcactaccg tcatcaataa acaaaacaag ctgatgtggt tcccgaggaa cttcgaaata
      121 aacggaatat tagcctccca ccttataatg gagtcgaatg tggaaaggtc gtgtgcccac
      181 agaaaacttt gctggcgcaa ctcgattgcc gaagggaaaa agaagaaaaa agcaagcaaa
      241 ataggcattt gaagcaacat cgggaaacat ccgctcatag ggcttgcccc agctttgtta
      301 tacagttcca tagtggcctg ttgacgtttg agcatcattt ccttatcctg tccgggatat
      361 ttggcttcaa tttctttgat ttgcgggcgt agcacacgca ttttcgcaga tgatttaaac
      421 gacttatatg tcaacggaag aatgatcaac ttgattaaaa gtgtcattat aagaataatg
      481 attcccattc cccaatcgaa tttcaggaag taattaaata cgggaatggt gaaatatttg
      541 ttgatccaac ttaaccattt gtatcctaaa tctaccagtt cttccagata aagtttctga
      601 ttgttctcct tgatcgcttt atcgtagttt ttcagtgtat aaaaatggtt tggcccgaaa
      661 tagtaattaa aagatgcggt gagttcgtct ttctgcgggt cggcagttac cggaacccag
      721 gtttcagcct tgtaatcctt gatgtgctcg gttgtactga gcggttggga tgtaaggatg
      781 gtgttgctga aaggtttttc ggcaataatg atcgtgctta agaactgatc tttgtatgca
      841 aaccatttca ccggttcggt aagctctttc cgctcgtttt tgctatcact taatttctgg
      901 acatcttgtc ccaggtacct gtagtttaat cgtgcaaaac ggttctcaaa ctggcgtccc
      961 tgctcttgct gacggacttt ttgttcccag ttgacacgga aatttgttaa actctccgga
     1021 tgcaaaccgt ttttcattcc ggctatccgg acatcaaagt ccagcatgaa ttcattggcg
     1081 ggaagagtgt agattaagtc aatgtgttgt tccggagaac tttctaaacg catcactacc
     1141 gactttccgt cggcggttgg tattggagta aaccattgat cttccgtgga taaacgaacg
     1201 ctgttgcggt taaagagttc caggttaaac cgggcttcgt caccttcgaa aagatagaga
     1261 ctgtcgccgt taaatttctt ctcgcctttg agttgtaccg aatgaatgct tccgcccaat
     1321 gtgcttaaga ccaatttaag cttttcattt tccagggtga caaattcttc tacggcggcc
     1381 tttatttctg ttgttgaatc ggttggagca ccaattgaat cattgacagc aggtgttccg
     1441 gttgcgaaaa aatcggtaac tctactttgc tgtttgattt cagaagtttg cctgggatca
     1501 tcggttgcca atcgctccgc ttctaacgca gccttttggg tttcgacttg ttgtattgaa
     1561 tcgcgcaacc tttgttgctc cgctatctgt tcctggctgg gccggtttaa aaacgtgaac
     1621 gcaataacga ttgcaccaat cagtaaaaaa ccaataattg tatttttatc cattcttaca
     1681 caataaaaga gagcgaattg aatcactctc gcaattattc aaaataatta tcttttttaa
     1741 tcgaaaccaa cattttcgtt gagtaaatca accagaagcc aaacttgccc ggataatgaa
     1801 cataggcgac tcaaaatggc acactttaat aaacttgttt tctcgaagct acaaaggtac
     1861 aaaaaaaagt aaaacgaata acagaagaac tgttttttag tgaatcagct atttgttatc
     1921 gacaaggtga aatatatttc ataattgata aaattattgt tttttcgtat catattaaaa
     1981 ttatatttgc caataacgta ttacttttgt agttttgtgc ccggaaataa aaaatattta
     2041 cgaaatttat gaatcctgtt acagagaaaa taaaaatggt gtgtgccggc aaaaatattc
     2101 cggcggaaga gctggcggct aatgcaggct tgactgtaga acagattgaa cttattttta
     2161 acagtgaaaa aataccctcg ctgtcatcgc ttatcaaaat ctccagggcg ctgggagtta
     2221 gattaggaac ctttcttgac gacaatgaaa acatgggccc tgttgtcaat aaaaactcca
     2281 ggcaatcgcc tccggttact tttaccagcc atttaaccaa tgccaattca cacatcgatt
     2341 ttttttcttt agcggcaagc aaatcaggaa ggcatatgga gccgtttctt atcgacataa
     2401 aaccttccta ttcatccaaa atgactactt cttcgcacga gggagaagag tttttgtacg
     2461 ttttaaaagg atcgataact gttttatacg gatcggataa gtttgttttg aataccggtg
     2521 aaagcattta ctacgactca gtagtagagc atctggtaat ctcggcaagc gatgagcctg
     2581 ctcaggtgct tgcggtagta tacaccccta attaattcac cgttttatgc aactttttga
     2641 gaagacacta ggtgattggc tcgagtactg ggccaatgaa acccctgata aagaatatat
     2701 tgtatattcc gatcgaaacc ttcgcttcac atggaagtcg ttcgatgaac gggtaaacaa
     2761 catggccaat gggctgttat cgatcggagt aacccgagga actaacgtag ggatttgggc
     2821 acaaaatgtt cccgattggt taacagtgct ttatgccacg gcaaaaatag gtgctgtggc
     2881 agtgactgtt aacactaact acaaaaccga agaactggct tttgtgctga aaaatgcgga
     2941 tatgcacacg ctatgcctaa ctgaaggaat ccccggtagt gattatattg atatgattta
     3001 tgaacttttg ccggaattga gaaccacaca acgcggaaaa ctcaaaaacg accgttttcc
     3061 cgggatgagg aatgtggtat atattggaca ggaaaaattc cgcgggatgt acaacacagc
     3121 tgagcttctt ttagtaggtg ccagccgtcc caaagatgag ttccgtgcgc tccgagagca
     3181 gaccggttgc cacgatatag tgaatatgca gtatacatcg ggaacaaccg gatttccaaa
     3241 aggcgtgatg ctcagccacc acaacatcac caacaacggt tatttaacgg gtgtccacat
     3301 gaaattcaca cccgatgata agtgctgtat ctgcgttcct ttgttccatt gttttggcgt
     3361 agtgttggct acgatgtgtt gcctcactca tgggagcacc caggtaatgg tggagagatt
     3421 tgatccgcta ttggttctgg cttctattca caaggagcgc tgtaccgtct tgcacggcgt
     3481 tcctactatg tttattgccg agttgaatca tcccatgttc cctttgttcg atatgagttc
     3541 gttgcggacg ggaataatgg ctggttcgct ttgtccggta gaactgatga agcaggtaaa
     3601 cgaaaaaatg ttcatggaca ttaccagcgt gtatggactt actgaaacct cgccgggaat
     3661 gacacaaacc catgttgatg atccattcgg ggtacgttgt tcaaccgtag ggcgtgccta
     3721 tgagtttacc gatgttaagg tgcttgatcc ggttacagga agagaatgtc ccgtaggagt
     3781 agaaggagaa atgtgctgtc gcggttataa tgtgatgaag ggttattaca aaaacccgga
     3841 agcaactgct caggtaattg atgctaatgg atacctgcat tcgggcgacc tgggtatgaa
     3901 agacgaagac ggcaattatc gtatcacggg aaggatcaaa gacatgatta ttaggggcgg
     3961 agaaaacatt tctcccaaag aagtagagga ttttctttat aaaatgcctg aagtcaggga
     4021 tgtgcaggta gtagcagtgg cttcacccag atatggggag gacgtgggtg cttttattat
     4081 cctaaaagag ggaaaagaac tgacgcgtga agatgtccgc gatttttgca aggatcatat
     4141 tgcccggtac aaaattccaa gatacgtgtt ctttatcagt caattcccaa tgacaggaag
     4201 cggtaaaata caaaaattcc gtctgcgcca aatggcattg gaaatgtgcg aagcgcaagg
     4261 cattgagata atttaagggt cgtcatcgac ctgaagaatg aatcagttcc ctcgcttttt
     4321 cgataatgga gtcttttcct tttgatttgt cactttcagt catggaaaca tatacatcgg
     4381 gttcgatacc gaattcgata tgttccttgt taacattgta catgggtgat gcggaaaacc
     4441 ggatagacca gccatttggt agttccgacg agaaaggtaa tccgctcccg ccgccggttt
     4501 tatccccaat aattgtagcg tttggggcat gtttcataac gtttacgaag tcgttcgtgg
     4561 cgctgtaaca acttctgttg gtcagaacaa ttaccggctt ttgatacctt atcttgtcgt
     4621 agctgttcac gtatttggga taaaaatcag aaaagtcgga atgcccttta ccaatcttgt
     4681 gtgaaatata tcctaccagt gttttttcgt tgaaaaatcg tgaagcaatt cgctctacgt
     4741 tggttaaact gcctcctcca ttgttgcgga catcaaagat gattccttta caaacagcca
     4801 tctggcttaa tacctgcgac atatttgcgt caccgatatc gttcgaaaag ctaccgtagt
     4861 aaacgtagcc gatattatcg tccaaaattt tataatacat tccaccggca atggcgaaat
     4921 ctcgctcaag gtattttttc tgaatattcg ggtcaaagtt taacgggaaa tcctccgtcc
     4981 aactccaata acgggacatg tcgtgcgagg caatcaagtt tacatgtccg tctttcagct
     5041 cggagagcat ttcacccatt aggttgaaaa gggcgtagtt tcccatatcg ttctttaccc
     5101 ttgaaccgta ctttttgtac acggcatccc agtcaattac cttgtattca aagaaacaat
     5161 aattctcatc gagaatcttc caaagtgcgt cgaaattact gcgtggatcg ttatcaaaac
     5221 gcatactgtc ttcgcatccc gaaacaacgg aaagcgaaaa gagaaaaatc aacaggtgtt
     5281 ttatttttct gaatatcatt gcgctaaggt aataacaatt ttttaaagtc atcaaatgaa
     5341 ttgcggtaat aattcaggtc gatatcgtta acaacaccgg aaatttttcc tgtatgcgaa
     5401 aattgccaca ttacccagtt cttatagtca ccgccgggtt ctttatgtaa gtcgcatatc
     5461 cataacgggt tgtctggaaa attatccttt atatacattt tatagcattc cttgttggta
     5521 tatattaccg gacgtttgcc gtattgttta aacagtgccg actcgaggtt tctgagctct
     5581 tttatcattc tcccgtgcga ctctttatcg ttgcaggcac ggttgatagc agaatgttca
     5641 acatctatgg ctggaatcat gtcgccggat tgcagcgtcg aatttcgtat gaaatgctgt
     5701 gcctgttttt taccgtcccg tccgaaagtg taaaaatggt aggagccaat tctcaggccc
     5761 gcatctttgg ctactttata atttttcttg taatccctgt ctttgtgctt cgatccttcg
     5821 gttgatttca gatagacaaa cgtaatgttt tcttcccgga gttggtgcca gttgatataa
     5881 gaattatgat gagagacgtc taccccgcga accaggtact tttgagagaa ctgtggatgg
     5941 tcaaaagaat gaaatatttc attcgtcgaa aagcggatga accgataaac gccaacggca
     6001 aaaagagcag ccagaaaaac gacaataagc caaacaacct gtcgtccatt tttgcttaat
     6061 tttagtcgtt tcccgcggct gtatgcagcc cgtcttcttt ttgccattgg cgttcaaata
     6121 caacaaactc tcttattcgc agggaatctt gttgattctt ctctcgtgtc taccaccttc
     6181 gaaaggcgta ttgagaaagg ttaccaggat ttcgtaaatt tcttcctcgt ttagcagcct
     6241 gcctggcaag gccagcacat tagcattgtt gtgagcgcgc gccagataag ctacttcttt
     6301 gttccagcag agggcggagc gaatatcccg gtgcttgttc atcgtcatat ttataccgtt
     6361 ccccgatcca caagcagcaa ttccaggata acattctccg ttctcaacgg ctgttgccag
     6421 gggatgggca aaatcggcat aatctgaact ttcttcggaa taggcaccaa aatctttgaa
     6481 aggaattccc ttttccgtca acagtttgat aacaatttgt ttggccgcaa aaccagcatg
     6541 gtctgaagcc aatccaatgg gtttctctgt tttatcgaat aaagacatga tttttttatt
     6601 taaactcaaa acttgttata gtaatttttt cacttgctca tacacgtttt tacccgtgta
     6661 gccgagtttt tcatccagga tcttgtaggg tgccgaaaat ccaaaagaat tcattcccca
     6721 tacttttccg tctgcaccga ccaggccttc tagcgttaca gggagtccag cggttaaacc
     6781 gaatttcttt ttgccttcag gcagaatcga ttcctggtat tctttggttt gactgcggaa
     6841 caatccttct gatggaacag agacgattcg cgtttttata ccatcggctt tcagtagttc
     6901 tgctccctct gcaagtgtgg agacttccga gcccgaagcc aggagaatca catcatagtc
     6961 attctcatcc tgtaccacgt aagctccttt ttcagcctgt aatgcttctt tgtaccggga
     7021 tcctgcagcg ggtagatcct tgatgttctg tctcgataag atcaaccctg taggcgtctg
     7081 tgcattttcc aacgccattt tccaggcgac tgtcgtttca tgcacatctg caggacggag
     7141 aacaagcatc gaattttgtc cgtggtgatt ttgtagcttt tccaacaacc ggatttgtgc
     7201 ttcttgttca acgggttggt gtgttggccc gtcttctcct actcgaaaag catcgtgagt
     7261 ccagatgaaa ataacgggcg ttcccatcaa ggcggctaca cgaattgccg gcttcatgta
     7321 atcggagaag gcgaagaacg taccgcacgc aggaataacg cctccgtgca aactcattcc
     7381 aatacacatg gccgacaggg tgaactcggc tacaccggct tgcaaaaatg ctccggagaa
     7441 atcattcttt tcaaatgctt tcgtatactt taaaaaaccg tctgttttgt cggaatttga
     7501 caagtcggct gaagcaacaa tcatgttttc tactttttga gccaaaacgc ccaggacggt
     7561 cgccgatgct gcacgagtag cgttatccgg tttttgcccg atagaggccc aatcgatttc
     7621 gggcagttcc cgggcaaacc agcggtgttt tttcgctgca agttcgggat tggcttttgc
     7681 ccatatattg taccttgctt ttttagcagc tacgatatcg ttgagttctt tttttctctt
     7741 atcgtaaagt gctgtaactt cggggaaaat aacaaaagga ttttccggat cacctcccag
     7801 gtttttaacg gtttgagcaa aatcagctcc tgctgcactt aggggttgtc cgtgtgtgga
     7861 tacctggcat tcaaacgatg tgcaatctgc tgccaaagca ccacgcccca tgatggtatt
     7921 accgataatc agtgtcgggc gttctttttc cgccttggct tccgttaacg ctttgcggat
     7981 ttcatccaca ttgttcccgt ctatcgtgat cactttccag ttccacgctt cgtatttctt
     8041 ggctacatct tcacttgtaa cggcgtctgt agtggtagaa agctgaatgt tattggaatc
     8101 ataaaacatg atgaaattat ccaatcccaa gtggccggca agccgtccaa ccccttgtga
     8161 aatctcttcc tgaataccac cgtccgtaat gtatgcataa atagtatgat caatggcatc
     8221 tcccaagcga gcttttaaaa acttagcggc aacagcggca ccggcaccgt aagcgtgtcc
     8281 ttgccccaga ggccccgaag tgttttcgat tccccgttcg atatcccgtt ccggatggcc
     8341 gggtgtgggg ctaccccact ggcggaatgc tttggtctcg tctatcgaga acttaccgct
     8401 taaacacaaa accgaataga acataggcga catatgtccc gcatcgagga aaaaccggtc
     8461 acggccttcc caataagggt tttcgggatc gtattccaga aattcgctgt agagaacgtt
     8521 aataaaatca gcaccaccca tagggcctcc cgggtgtcct gattttgctt tttctaccat
     8581 tgatgccgac aggatgcgga tattatcggc cgctttgttc attaatgcat tgtcattcat
     8641 aagattagga aatatttatt tttatcagtt tctaacggat ccttattgat gtaattcgtg
     8701 gaacagtttc ttatcataca aagatacggt tttttaaaaa taaaggggtg tgaaaattga
     8761 catcattttg tggatatttg actcttttaa tttattaact acctttgcaa cgtttaatac
     8821 aacagaaatg ccgctgagta aaaacagaat aaaacagatt cggtctttaa gtgaaaaaaa
     8881 gtaccgcagc gaacacggta cgtttgttgc cgaagggaaa aaattggttc tcgatttgct
     8941 tggtaattgc cggtgccagt ttttagccgg attaccggat attttgcagg agatcccgcg
     9001 tttatcggca gaagagatgg tagaagctac ccccgaggaa ctgaaaaagg catctcacct
     9061 gaaaaccccg ccgcaactta tcggtatttt ttatcagccc aaaaacgctc ttgaagaaat
     9121 tgagtttgtt ggtaagttgc accttgtcct cgacggaatt caggacccgg gtaatatggg
     9181 taccattgtc cggttgagcg actggtttgg cattcaacac gttttctgtt cacccgatac
     9241 cgccgacatt ttcaacccca aaacagtaca agctaccatg ggggctattg cacgggtcaa
     9301 tgttcattat gtagacctga aagacttgtt aactagaaat aactcattgc ctgttttcgg
     9361 tacattactg gaaggtgaaa acatttacaa agcagaatta ccgaaaaacg gatttattgt
     9421 tatggggaac gagggaaaag gaatttcccc cgatattcaa aaactcatta cccataaact
     9481 gtttattcct aattatccaa caaattctca aacatcggaa tcactaaatg tggctgtggc
     9541 tgcagccatt gtgtgctccg aattcagaag aagggtctga accggcagat tcagtactcc
     9601 tgccagcttt tgattttcaa ctgctctaca tccaacatgc agaaagcagt aataaaagcc
     9661 gatgccaacc gtgcgttggt gataagcgga acgttaaaat ccaccgcagt gcgccggata
     9721 atgtaaccgt tggtaagctc gcgtcgggta acatccttgg gaatattgat aatcagatcg
     9781 aacttattgt gggtaatcat ctcctgaatg ttgtgttcgc ctccttcatc cggccaattc
     9841 acatcggtag ctgcaacgcc gttatcggtc agaaattgtt gtgtcccgtg cctcgcatac
     9901 aagtcgtacc ctttggcatg gagcagtttg caggcttcca acaaa
//
LOCUS       DAOSDR010000057         9943 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrimonas sp. isolate AMR_MDS_1826
            NODE_2569_length_9943_cov_4.314422, whole genome shotgun sequence.
ACCESSION   DAOSDR010000057 DAOSDR010000000
VERSION     DAOSDR010000057.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863355
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrimonas sp. (wastewater metagenome)
  ORGANISM  Petrimonas sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Dysgonomonadaceae; Petrimonas.
REFERENCE   1  (bases 1 to 9943)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9943)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:10:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,720
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 35
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9943
                     /organism="Petrimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2569_length_9943_cov_4.314422"
                     /isolate="AMR_MDS_1826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2023866"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1673)
                     /locus_tag="PLI30_03625"
     CDS             complement(<1..1673)
                     /locus_tag="PLI30_03625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006799884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HOI78676.1"
                     /translation="MKRILLFFIVLIHITAVSSQHNSWPRPSVECKPWTRWWWLGSAV
                     DPPNLSYNLEELARAGMGGVEITPIYGVKNGEPQYINYLSPKWMEMLSHTVSEAHRLG
                     MEVDMNTGTGWPFGGPGVTVEDAATKALFQEYKVKGGTRMEMKITIGDEEQRKVAYLS
                     KLYAFSDDGQKTDLTSSVGQDGILDWLAPAGNWRLIALFVGKTFQQVKRAAPGGLGYV
                     MDHFNKSAVKNYLNRFDDAFSRSGAPWPNAFFNDSYEVYGANWSPGFLEEFEQRRGYK
                     LEEYLPELLGKGQTDTSRRVISDYRETMGEMLLENFTVSWTEWANRHGATTRNQAHGS
                     PANILDLYATVDVPECEIFGITGFDIPGLRKDSLIKINDGDPVTLKFASSAAHISGKN
                     YTSSETFTWLTEHFRTSLAQCKAEIDQVFTSGVNHVFFHGTPYSPREAAWPGWLFYAS
                     VNLSPTQPVWKDAPAFFSYINRVQSFLQYGKPDNDLLVYFPVYDIWYEQQDDFYFAFS
                     IHGLREKLPRFYQTVEKIRTGGRDVDYISDRFIRSSSVEDGLIKTAGGAT"
     gene            complement(1688..1894)
                     /locus_tag="PLI30_03630"
     CDS             complement(1688..1894)
                     /locus_tag="PLI30_03630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI78677.1"
                     /translation="MRIEKLEMDNIWFNPCRENFFNKNYYLYRENNLFPSPSIDTGGT
                     PGPVSGTGSYSGRENMKQYRKGIE"
     gene            1953..3485
                     /locus_tag="PLI30_03635"
     CDS             1953..3485
                     /locus_tag="PLI30_03635"
                     /EC_number="6.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005829355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA carboxylase subunit beta"
                     /protein_id="HOI78678.1"
                     /translation="MNKRKIFEQRRQFLQEMEEVAVQKQHAKLKLTARERLNVLFDEG
                     SFFELNTFVEPAKAIKKTQNAFGDGVVVGRGLIQGRSVFAYAQDFTVMGGSLGFAHGM
                     KIAKVQEMALKFGSPCVGLIDSGGARIQEGINSLSAYARIFRNNVSSSGIIPQISVIL
                     GPSAGGAVYSPAMTDFVIMTNKTSYMFVTGPDVVKEVLNQNISMDELGGGNIHAEKSG
                     VAHFVFEDEEHAIIQVRKLLAYLPLNNFDPVPVSMDVSFNAERQEYLNKIVPDDPNRP
                     YDMKDIINILVDNNTFLEIHEKFAQNILVGFARLDGKTIGIVANQPKVMAGTLDINAS
                     IKGARFVRFCDSFNIPILVLEDVPGFMPGSEQEHTGIIRNGAKLLYAFCEATVPRVTV
                     ITRKAYGGAFIVMNSLGIGGDFNFAWPTAEIAVMGPAGAIKIVNKAELAAASDREAVE
                     KELVEKYKDEIANPFKAEELGMIDDVIKPSKTRQVLITAFDILANKQYSQPERKHGNI
                     PL"
     gene            3536..5032
                     /locus_tag="PLI30_03640"
     CDS             3536..5032
                     /locus_tag="PLI30_03640"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HOI78679.1"
                     /translation="MNKKILIANRGEIAIRIIRSAKKLGLTTCVIQGNREPDAMYLEY
                     ADVIIPVRENLTGSIIFLNPEEIVRLALEHQIDMIHPGYGFLSENPDFAALCEQNGIT
                     FIGPSSDLIQNMGIKTIAKKMAAEAGMPLVPGSAGAITDAQVAKEFADTIGYPVILKA
                     SAGGGGRGMRIVEKAETMERHFKSAYDEAVAAFGNGDIFIEKYLTNPKHLEFQILGDR
                     YGHVIHLGERECSLQRKHQKLIEEAPSASIDSKTREQMGKLAVKFAQSIGYYSAGTIE
                     FILDEDGSYYFMEMNTRIQVEHPVTEMITGVDLIEWQIRIALGEKLRLKQKEIRLNGW
                     AIECRVNTEDPQNRFTPQTGFIERVFFPHGDHIRVETGVKDFSVVTPYFDSMIAKIIV
                     HGENRDDCIDKTLNALKEFSISGLKTTVPFCRTVLRSKEFREATYTTHWIDSVFTTDM
                     LESEDEAMMAALAATITYAKEYLQYSSDSPMFKSESLNVWVLNKRINK"
     gene            5048..5596
                     /locus_tag="PLI30_03645"
     CDS             5048..5596
                     /locus_tag="PLI30_03645"
                     /inference="COORDINATES: protein motif:HMM:NF012584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI78680.1"
                     /translation="MATSYVYKRDATRLFIAMRDNGDRYKIYLPKESKPIINGEEKDV
                     FFIEHKNNFSSFSMGDATYRCEITQRDQHRIMVKVNGVEYKFSIESIFSYLRRNLLNK
                     GVEVQAGNNLKSPMPGKIVDIFVSEGDLVNEGEPVLRLEAMKMQNEITACCNGVIRKI
                     HVAPGQSIMQDELLVEITSIDE"
     gene            complement(5611..6774)
                     /locus_tag="PLI30_03650"
     CDS             complement(5611..6774)
                     /locus_tag="PLI30_03650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HOI78681.1"
                     /translation="MGKYKIKTLNGISESGLEKFGQNFEVGEKMDAPHGIILRSASLH
                     EMEFPDSLHCIARAGAGVNNIPVDKCAEKGIVVFNTPGANANAVKELAIAAMLLSSRK
                     IVEGINWTRSLTGDDVPKQVEKGKAQFVGPEIRDKKLGVIGLGAIGVMVANAAVNMEM
                     DVYGYDPYISVDHAWGLSRSVKKANDLKKVFKHCDYITLHIPLTDETKGMLNKEAFAQ
                     MKEGVRIINLSRGELVNDADMIDALEAGIVSEYVTDFPNEKMINVPGVIPVPHLGAST
                     PESENNCAVMAVLQTKDFLENGNIRNSVNFPVCDMGISIVKHRLTIAHKNIPKMLGQI
                     SAVLAEQGTNIANMINRSRGDYAYTMVDLEEEITDENVKRIKDIAGVLKVRVI"
     gene            complement(6924..9596)
                     /locus_tag="PLI30_03655"
     CDS             complement(6924..9596)
                     /locus_tag="PLI30_03655"
                     /EC_number="7.2.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019537798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005388 - P-type calcium transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006816 - calcium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-translocating P-type ATPase, PMCA-type"
                     /protein_id="HOI78682.1"
                     /translation="MKPFFNQPVGEVLQQFNVSPDQGLSSAEAKKKLEEAGPNQLASK
                     KQKSIAVIFFEQFRSSMVVILLIAAAVSGVIGVMEGEGLTETFIILAILIVNAIIGVM
                     EETKAQSSLDALNKMSAPRSKVLRDGEVTETVSTQLVPGDIVILDTGDIIPADMRLIE
                     AVNLKIQESALTGESVPAEKSTEAIDTEDVPLGDRDNMAFSTGVITYGRGKGVIVSTG
                     MDTEVGKIAGMLQSAQETETPMGKRLEQLGKILGYVALGICVLIFAVGMLYGNHWLEM
                     FMMAVSLAVAAIPEGLQIVSTIVLAIGVQRLVKLNAIVRTLPSVESLGSTTVICSDKT
                     GTLTQNKMTVVEGMVSGNRCDFRNPPVPEELSDDERILLNSSLLCTDAHLKMLPDGTH
                     ENAGDPTETAIVDIALALNLNKNEEDRKYPRVSEVPFDSERKRMATVNQMADGKLRVN
                     VKGGLDEVLAVTTHILMHGKVRTITEEDITTIRNENNRMAKSALRVLSVAYRDIDRLP
                     DRVDAETIERNLVFIGMLGMIDPARPEVVEAVKKCKTAGIRPVMITGDHKVTAVAIAA
                     EIGIYTEGDKAVAGNVLQEIPDEELYRDIEKYSVYARVAPEHKVRIVKAWQSHGDIVA
                     MTGDGVNDAPALKQADIGVSMGIVGTEVAKDASDVVLTDDNFATIVGAVEEGRRIYDN
                     ILKAIQFLLSANVGEVLLIFIASVFNLGNPLTPIMILWINLVTDSLPALALSMDPAEK
                     DIMTRHPRDPKRGFFTRGMTWRIFYQGMTIGLISLAAYRIGFNDGGQQLGQTMAFAVL
                     AFSQLLHVRNLHSNRRSSFRTSPMSNKALVLAILASAVLMLIVLFLPAIRDIFKIVEM
                     DGVHWLYVVGLSFVPIVVVEAVKLLGINHTRDEY"
BASE COUNT         2625 a         2575 c         2249 g         2494 t
ORIGIN      
        1 gtagcaccgc cagccgtttt aataagtccg tcttcgactg acgacgaccg gataaaccgg
       61 tctgaaatat aatccacgtc cctgccgccc gttcttattt tctcaaccgt ttgataaaat
      121 cggggaagtt tttcgcgtaa gccgtgtatg gaaaaagcga agtagaaatc gtcctgctgc
      181 tcgtaccaga tatcgtaaac cggaaaatag accaacaggt cgttatccgg ctttccgtat
      241 tgaaggaacg attgcacccg gtttatgtag gagaagaagg ccggtgcatc tttccagacg
      301 ggctgggtag gcgacaggtt caccgatgcg taaaaaagcc agcccggcca tgcagcttcc
      361 cggggcgaat aaggcgttcc gtgaaaaaag acatgattta cgcccgaggt aaacacctgg
      421 tctatctcag ccttgcattg agccagtgag gtccggaagt gttccgttaa ccaggtaaat
      481 gtttccgatg aggtataatt tttccccgaa atgtgggcag ccgaactggc aaatttcaag
      541 gttaccggat ccccgtcatt tattttaatc aggctgtctt ttcgtaatcc gggaatatca
      601 aaacccgtta ttccaaaaat ctcgcattcg ggaacgtcca cagtagcata aagatccagg
      661 atattagccg gagatccgtg tgcctggttc cgagtggtag caccgtgcct gtttgcccac
      721 tccgtccagg aaaccgtgaa attttccagc aacatctctc ccatcgtttc ccggtaatcg
      781 gaaatcactc tacgggaggt atctgtttgt cctttaccca ataactcggg caggtattcc
      841 tccagcttat atcctcttcg ctgttcaaat tcctccaaaa agccgggact ccagtttgct
      901 ccgtaaactt cgtaagaatc gttgaaaaat gcgttcggcc acggggcgcc gctgcgagaa
      961 aaagcatcgt caaacctgtt gaggtaattt tttacggcac ttttgttgaa atgatccatt
     1021 acatacccca ggccacccgg cgcagcacgc tttacctgct gaaatgtctt cccgacaaac
     1081 aacgcaatca gcctccagtt ccccgccggt gccagccaat ctaaaatgcc gtcctgaccc
     1141 acggaggaag tcaggtctgt tttttggccg tcatcggaaa aagcgtacag cttagacagg
     1201 tatgccactt ttctctgttc ttcatccccg atcgtgattt tcatctccat tcttgtacct
     1261 cccttcactt tatattcctg aaacagtgct tttgttgctg catcctcaac cgttacaccg
     1321 ggacccccaa aaggccatcc ggttcccgta ttcatgtcta cctccatgcc cagccggtgc
     1381 gcttcggata cggtatggga aagcatttcc atccatttcg gagagagata gtttatgtac
     1441 tggggttcac cgttctttac cccatagatc ggcgtgatct ctactccacc catgcccgct
     1501 ctggccagtt cttcgagatt atagcttaaa ttgggtgggt ctaccgcgct tcccaaccac
     1561 caccagcgtg tccacggctt acattcgacg gaaggccggg gccatgaatt gtgttgggaa
     1621 cttacggctg taatatgtat taacacgata aaaaaaagca ggatcctctt catttgaata
     1681 gagttgatta ttcgattcct ttccggtatt gtttcatgtt ctctctgcca ctatagcttc
     1741 ctgttccgga tacgggtccc ggagttccgc ccgtatcaat gctcggagaa ggaaaaaggt
     1801 tgttctcccg gtaaaggtaa taatttttat taaaaaaatt ttcccggcac ggattaaacc
     1861 aaatattatc catttcaagc ttttcgatac gcattaagaa actttttttt tatccagctg
     1921 tttattgttt gctataccgt caataaaatc atatgaacaa aagaaaaata ttcgagcaac
     1981 ggaggcagtt tttgcaggaa atggaagagg ttgccgtaca aaaacagcat gccaagctca
     2041 aacttactgc acgcgaaaga ttaaacgtgt tgttcgacga agggagcttc ttcgaactca
     2101 atactttcgt tgagccggct aaagcgatta aaaaaactca aaatgcattc ggagatggag
     2161 ttgtggtagg gcgtggattg atacagggac gcagcgtgtt tgcttatgcc caggatttta
     2221 ccgtaatggg cggatcactg ggttttgcgc acgggatgaa aattgccaag gttcaggaga
     2281 tggcgcttaa gttcgggtct ccctgtgtag gcctgatcga tagcggcggc gcgcggattc
     2341 aggaaggcat caacagttta tcggcttacg cgcggatctt cagaaacaac gtcagttcgt
     2401 ccggaattat tccgcagata agcgttattc ttggcccgtc cgctggcggt gcggtgtact
     2461 ctcccgccat gaccgatttt gtgattatga ccaacaaaac gtcgtacatg ttcgttacgg
     2521 gacccgatgt ggtgaaggaa gtccttaacc agaatatcag tatggatgag ctgggcggag
     2581 ggaatatcca cgcagagaaa agcggcgtag cccacttcgt gttcgaagac gaagaacacg
     2641 ccatcataca ggttcgcaaa ctgctggcct accttcccct gaacaatttc gatccagtcc
     2701 ctgtttccat ggatgtcagt ttcaatgcgg agcgtcagga atatctgaac aagattgttc
     2761 ccgatgatcc gaaccgtccg tacgacatga aggatatcat caatatcctg gtagacaaca
     2821 atacttttct agagattcac gagaaatttg cacaaaacat actcgtcggt tttgcgcgac
     2881 tggatgggaa aacaattgga atagtggcaa accagcctaa ggtaatggca gggacattag
     2941 acataaacgc cagcataaaa ggcgcccgtt ttgttcggtt ctgcgacagc tttaacattc
     3001 ccattctggt gctggaagat gttcccggat ttatgcccgg gtctgaacag gaacataccg
     3061 gaattatccg caacggggcc aagctgctat acgcattttg tgaagctacg gttccccggg
     3121 tcaccgtcat tacccggaaa gcatacggcg gagcatttat cgtgatgaac agcctgggta
     3181 tcggcggcga ttttaatttc gcctggccaa cggctgaaat tgcagtgatg ggccccgccg
     3241 gagccattaa aatagtaaac aaagccgagc ttgccgcagc ttccgaccgg gaagctgttg
     3301 aaaaggagct ggtggaaaag tacaaggacg agattgccaa cccgtttaag gcggaagaac
     3361 tggggatgat cgacgatgtg atcaagcctt cgaagacccg gcaggtgctc atcaccgcct
     3421 ttgatatttt agccaacaaa cagtattccc aacccgagag gaagcacgga aacataccgt
     3481 tgtaatatga cgcaggggct tgttggacag cctctgcaaa aaactgaatc acagcatgaa
     3541 caaaaaaatt cttatcgcca acagaggtga aatagctata cgcattatcc ggtcggcaaa
     3601 aaaactaggc ttaactacct gcgttattca gggtaaccgt gagcccgatg ccatgtacct
     3661 ggaatatgcc gatgtaatca ttcccgtgcg ggaaaacctg accggcagca tcattttcct
     3721 caatcccgaa gagatcgtca ggctcgctct tgagcatcag atcgacatga tccatcccgg
     3781 gtacggattc ctttcggaga acccggattt tgccgccctc tgcgaacaga atggcattac
     3841 gtttatcggc ccttcttccg atttgataca aaacatgggc atcaagacca ttgcaaaaaa
     3901 aatggcagcc gaggccggaa tgccgctcgt cccaggcagt gcaggcgcca ttaccgatgc
     3961 acaggtggcc aaagagttcg ccgacacaat cgggtacccg gtcattctta aagcatcagc
     4021 gggtggtggc ggacgcggaa tgcgcatcgt ggaaaaagcc gaaaccatgg agcgccactt
     4081 caaatcggcc tacgatgaag cggtggctgc cttcggtaac ggcgatattt ttatcgagaa
     4141 atacctgacg aaccccaaac acctggagtt tcaaatcctt ggagacagat acgggcacgt
     4201 cattcattta ggtgaaaggg aatgctcctt gcaaagaaaa caccagaaac tgatcgaaga
     4261 agcaccctcg gccagcatcg attccaagac aagggaacaa atgggcaagc tggccgtgaa
     4321 gtttgcccaa tccattggat actactctgc cggcaccatc gagtttatcc tcgacgaaga
     4381 cggatcctac tatttcatgg agatgaacac ccgcatccag gttgaacacc ctgtcacaga
     4441 aatgataacc ggggtagact tgatcgaatg gcagatccgg atcgccctgg gcgagaaact
     4501 ccggctgaaa caaaaagaga tcaggcttaa cggatgggcc atcgaatgca gggtaaacac
     4561 ggaagatccg caaaaccgct tcacgccgca aaccggattt attgaaaggg ttttctttcc
     4621 acacggagat catatacgcg tggaaaccgg cgttaaggat ttctcagtgg tcacccccta
     4681 cttcgattca atgattgcca agatcatcgt tcacggcgaa aaccgggacg attgtatcga
     4741 taaaaccctg aatgctttaa aagaattttc aatttcggga ttgaaaacaa ctgtgccgtt
     4801 ttgccggact gtcctgcgca gcaaggaatt cagggaagct acgtatacca cacactggat
     4861 agactcggtt ttcaccaccg acatgctcga atcggaagac gaagccatga tggcagcgct
     4921 ggcagcgacc atcacttatg cgaaagaata cctccaatac tcgtccgatt ccccaatgtt
     4981 caaaagcgaa tcactcaacg tctgggtact caacaagaga attaacaaat aaacggtaat
     5041 taacaaaatg gcaacatcat acgtatacaa aagagatgcc acccgcttgt ttatcgccat
     5101 gcgggacaac ggcgacaggt ataaaatcta cttgcccaaa gagtcaaaac caatcatcaa
     5161 cggtgaagaa aaggacgttt ttttcattga acacaaaaat aatttttcgt ccttcagtat
     5221 gggcgatgcc acataccggt gcgaaataac ccagcgcgac cagcaccgga tcatggtaaa
     5281 ggtaaacggc gtggaataca aattcagcat cgagtctatc ttctcctact tgcgacgtaa
     5341 tttgctcaat aaaggagttg aagtacaagc cggcaacaac ctgaaatcgc ccatgccggg
     5401 aaaaattgtc gatatttttg tcagcgaggg cgatctggtg aacgaaggcg aaccggtatt
     5461 gaggctcgag gcaatgaaaa tgcaaaacga aatcactgct tgttgcaacg gtgtaatccg
     5521 caaaatacat gttgcaccgg gacaatcgat catgcaggac gaactgctgg tagagattac
     5581 ttctatagac gagtaaccgg taagaaacag ctagataacc cgcactttta agacacccgc
     5641 aatgtctttg atgcgtttca cgttttcgtc ggtaatctct tcctccaggt ccaccatcgt
     5701 gtaagcgtaa tcaccgcggc tgcgattgat catattggca atattggtgc cctgttcagc
     5761 cagtacggca gaaatctgcc ccagcatttt gggaatattt ttgtgtgcaa tagtcaaccg
     5821 gtgttttacg atactgatac ccatgtcgca gaccggaaaa ttcaccgagt tccggatatt
     5881 cccattctcc aggaaatcct tcgtctgcag tacggccatc acggcacagt tgttttcgct
     5941 ttccggagta gaggcaccta agtgcgggac aggaataaca ccggggacat tgatcatctt
     6001 ttcgttggga aaatcagtta catactcgct cacaataccg gcctccaaag catcgatcat
     6061 gtccgcatcg ttcacaagct ctcccctgga aagattgatt atccgcactc cctctttcat
     6121 ttgagcaaac gcctccttgt tgagcatacc ttttgtctca tccgtcaaag ggatgtgcaa
     6181 cgtgatgtaa tcgcagtgct tgaaaacttt cttcagatcg ttagcttttt tcaccgaacg
     6241 cgataaaccc catgcatgat caacagaaat atagggatcg tatccgtaaa catccatttc
     6301 catgtttacc gctgcattgg caaccattac tccaatagct ccaagtccaa ttacacctag
     6361 ctttttgtcg cgaatttccg gtcctacaaa ttgcgctttt cctttctcta cctgcttggg
     6421 cacatcgtcc cctgttaacg accgtgtcca gttaatccct tccacaatct ttctcgacga
     6481 caaaagcatg gcagcaattg cgagttcctt taccgcattg gcattggccc ccggcgtatt
     6541 gaaaacaacg attccttttt ccgcgcattt gtctacgggg atattattaa ctccggctcc
     6601 ggcacgtgct atacaatgca aactatcggg aaattccatc tcatgcaacg acgcgctgcg
     6661 taagatgatc ccatgcggtg catccatttt ttcacctact tcaaagtttt gtccgaattt
     6721 ttccaacccc gattcggaaa ttccgttcag ggttttaatc ttgtacttac ccattattta
     6781 ttgttaatta gagaattact atcgacgagc agatgttttt attatttgaa acaaatgact
     6841 tcaaatttcg aacgcaaaga tataaaatct tcatctcatt ttacaattta ttacaaaaaa
     6901 tagtaagaat aagtcaagtt gtattaatac tcatcccggg tatggtttat ccccagtaat
     6961 ttcaccgctt ctaccacaac aatcggaaca aacgacagcc cgacaacata cagccagtga
     7021 actccatcca tttcgacaat cttaaagatg tcacggatgg cgggtaaaaa caatacaatg
     7081 agcatcaaaa cagccgacgc gagaatggca agcactagcg ccttgttgct catcgggctt
     7141 gtcctgaaag acgaccgcct gttggaatgt aggttacgca cgtgcagcag ctgtgaaaaa
     7201 gccagtacag caaacgccat ggtctgcccc agctgttgtc cgccatcgtt gaatcctatc
     7261 cggtaagcgg ccagcgagat cagaccaatg gtcattccct ggtaaaaaat gcgccaggtc
     7321 attccccggg tgaagaagcc ccgcttcggg tcacgcggat ggcgggtcat aatgtctttt
     7381 tctgccgggt ccatgctcag cgccagtgcc ggcaggctat cggtaaccag gtttatccag
     7441 aggatcatga taggcgtaag cggatttcca aggttgaaga cagaagcgat aaaaatcagc
     7501 aatacttcgc ccacattagc ggagagcagg aattgaatgg ctttgagaat attatcataa
     7561 atgcggcggc cctcttccac agccccaaca atggtggcaa aattgtcatc ggtaagcact
     7621 acatccgatg cgtccttggc cacctcggtc cccacaatcc ccattgagac gccaatatca
     7681 gcctgcttaa gcgcaggtgc atcgtttacc ccatcaccgg tcattgccac aatatctccg
     7741 tgtgattgcc atgcttttac tatcctcacc ttgtgctcgg gtgccacacg cgcataaacc
     7801 gagtatttct ctatgtcacg ataaagttcc tcgtcgggta tttcctgcaa cacatttccg
     7861 gccactgcct tatcgccttc ggtataaatc ccgatctcag cggcaatggc cacggctgtc
     7921 accttatgat cacccgttat catcaccggg cgtattcctg cggtcttgca tttctttacg
     7981 gcttccacca cttcgggacg ggccggatcg atcatcccca acataccgat aaacaccagg
     8041 tttctttcaa tggtctctgc atctacacgg tctgggagcc ggtcaatgtc tctatacgcc
     8101 acggaaagaa cccgcagcgc agatttagcc atccggttgt tttcgttccg gatcgtggtt
     8161 atgtcttctt cggtaatggt acggactttc ccgtgcatca ggatgtgcgt ggtaaccgcc
     8221 agcacttcgt cgaggccacc cttgacgttc acacgcagct tcccatcggc catctgattg
     8281 acggtagcca tccgcttccg ttccgaatcg aaaggcactt ctgaaacacg cggatacttc
     8341 cgatcctctt cgttcttgtt caggtttagg gcaagagcaa tatccacaat agctgtctcg
     8401 gtaggatcac cggcgttttc gtgtgtaccg tcgggcaaca ttttcaggtg ggcatcggta
     8461 caaaggagcg atgagttcag caggattctc tcatcgtcgc ttaactcttc aggaacgggc
     8521 ggatttctga aatcgcatcg atttccgctt accattcctt ccaccacggt cattttattt
     8581 tgtgtgagcg ttcccgtctt atcggagcag atcacggtgg tacttcccaa cgactcaacc
     8641 gacggcagtg tacgcacgat ggcattgagc tttaccaaac gctggacacc aatggccagc
     8701 acaatggtcg atacgatctg caaaccttca ggaatagctg ccacggcaag gcttacagcc
     8761 atcataaaca tctccagcca atgatttcca tacagcatac ccacggcaaa aatcaacaca
     8821 cagatgccaa gcgccacata acccagaatt tttccgagct gctccaatct ttttcccatc
     8881 ggagtttccg tctcttgtgc actttgcagc ataccggcaa tcttacccac ctcggtatcc
     8941 attcccgtgc taactatcac tccttttccc cgaccgtaag ttattacacc ggtagaaaaa
     9001 gccatgttgt cacggtctcc caacggaaca tcttccgtat caattgcctc cgtacttttt
     9061 tccgccggca ccgattcccc cgtaagtgcc gattcctgaa tcttcaagtt taccgcttcg
     9121 ataaggcgca tatccgcagg gataatatca ccggtgtcaa gaatcactat atcgcccgga
     9181 acaagctgcg tagataccgt ttccgtcact tcaccgtccc gcaatacttt tgaccgcggt
     9241 gcgctcatct tgttcagggc atcgagcgat gactgagcct tggtttcttc catcacgccg
     9301 ataatagcat ttacgataag gatagcaagg ataatgaagg tttcggtcag cccctctccc
     9361 tccatcacgc cgataacacc cgacacagct gcggctataa gcaatataac caccatactg
     9421 cttctgaact gctcaaaaaa gattaccgct atggattttt gtttcttgga cgccagctga
     9481 ttaggccctg cctcttcgag tttctttttt gcttcggcag aagaaaggcc ttgatctgga
     9541 gatacattga actgttgcaa cacctccccg acaggttggt tgaaaaatgg tttcatagtt
     9601 gtttgttttg tttgcggtca ttaagaaatt acttattcag ctttcactcc gctccagtcg
     9661 aaagcaccgt acctgataaa acaaaccgga ggataaattg tttggaatgc ccttaagccg
     9721 tgcgacaacc ggacaaatac cggcagaggc gccataaatg acgcctctgt caaccgatac
     9781 ggccaatccg tcaaaaatca aaactcagga taattccgtt gacggttccg ctaatgctgt
     9841 cgcacgttcc gtcggctttg atggatccga gcaacagaac gtcatattca atgccgttgc
     9901 agcgataacg gccttctatc cgaaagtgtg agccagacgg atc
//
LOCUS       DAOSDR010000058         9753 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrimonas sp. isolate AMR_MDS_1826
            NODE_2616_length_9753_cov_4.681687, whole genome shotgun sequence.
ACCESSION   DAOSDR010000058 DAOSDR010000000
VERSION     DAOSDR010000058.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863355
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrimonas sp. (wastewater metagenome)
  ORGANISM  Petrimonas sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Dysgonomonadaceae; Petrimonas.
REFERENCE   1  (bases 1 to 9753)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9753)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:10:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,720
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 35
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9753
                     /organism="Petrimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2616_length_9753_cov_4.681687"
                     /isolate="AMR_MDS_1826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2023866"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..929
                     /locus_tag="PLI30_03660"
     CDS             <1..929
                     /locus_tag="PLI30_03660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019538482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HOI78683.1"
                     /translation="KQRITMMNKTQSPAVKLAKYLTVIPLVLLLITLNSCLSKEKQTD
                     EKSNDQTEELQNSSSKNDIKADVSEGYVPPVTDSQEEIFSVVEEQPLYPGGNAAMMKF
                     LSNNIKYPVAAQEKGTQGRVITNFVVEKDGSLSDIQVIRGVDPSLDAEALRVLKTMPK
                     WKPGKQKGEEVRVRFTLPVVFRLQSPPPPPPPPPTPVKESGSNDVFVVVEKQPEFPGG
                     NAAMMKFLSNNIKYPVAAQEKGIHGRIITNFVVEKDGSLNEVKVVRGVDPLLDAEAVR
                     VIQSMPNWKPGIQKGETVRVRYTLPVVFRLQQ"
     gene            942..3455
                     /locus_tag="PLI30_03665"
     CDS             942..3455
                     /locus_tag="PLI30_03665"
                     /inference="COORDINATES: protein motif:HMM:NF017390.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M56 family metallopeptidase"
                     /protein_id="HOI78684.1"
                     /translation="MEALSAYIIKVNIALIFFYLLYVIFLKKDTFFAFRRYFILAATV
                     FSFVYPFITVSAWENIISFEKPQKYTTQVTVEDPAFAVVLADEVQTAEPVNTININWW
                     QVLLLLLSAGVVFFLARFTWQLVSILQIKHKSIRKNIDGMEVFDLYDEITPFSFFRWI
                     FIHLDSHSDTEIKQILLHEQTHARQWHSVDIILMELLCVFLWWNPIIWLMKRETAINL
                     EHLADNGVLRKGVNSRDYQYYLLRLTYHQNATRIVNNFNVSLLKQRITMMNKTKSPAR
                     KLVKYLIALPLALLLMAGNSVYAQNQRESEIFTEVEKYPEFPGGNQAFMKYLVESIKY
                     PVKAQENNEQGRVITNFVVEKDGSISNIKVIRGVTAALDAEAVRLLNSMPNWQSGEHQ
                     GKPARVRFTLPIVFRLQGGSAGKSQSPVTPVSADVNEKGKILDEVVVVGYGPTHRESI
                     PSPQEYAESNGNELFIVVEVQPQYPGGDITMNKFISDNLQYPKIAQENGIQGRVITQF
                     IVEKDGSLSDIQVVRGVDPSLDKEAIRLIRLMPEWIPGTQRGNKVRVRYTLPVTFRLS
                     DEAPTVEAAKGVQGKLNDPVIKIGSSDATFVVVEEQPEFPGGNAAMMNFITENIRYPK
                     EAHEKGIQGRVITKFIVRKDGSIDSVQIARGVDPLLDAEAIRVLESMPTWKPGKQRGQ
                     PVNVMFTLPVVFRLDKKTENGIQKSQTSSKNVQNPEFPGGENAYMKFLSDNIKYPVIA
                     QENGIQGFVDAVFNIDTKGKVTFVKFNRSVDPSLDKEVKRVIELMPDWIPGKFNGAAT
                     SITSGASFIFRLQGDGVDEYKGPTPSNAIVVVGYGSSKK"
     gene            3585..4892
                     /locus_tag="PLI30_03670"
     CDS             3585..4892
                     /locus_tag="PLI30_03670"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOI78685.1"
                     /translation="MKTEFAISVVLTMVSVSVSAQTLTAHDFMQQYRYREAIEWLETQ
                     PETAENLLLKAESHERLYNHTDALPVYERLLLNNPDDVALLISAAECASQAGDTESAL
                     KYWIKADSLSPENLFFQTKKAIAYYRNGNWQETIESTQTVFQSDSIPLLLRMTGDAFQ
                     NIHEPIWANYFYTKAYEKNPSDHLSLSRICEYFYAMEEDGYDTVAVMTEKYLNEINPN
                     QKTIGQLNGMANYSSGRFDKAIDRLKAIVEIGDSSYTTTYFLGMSNYAKGWYYEAVKW
                     LGKAYELNRNDVNMFYYYGTALGRTKNRERAVEILQEGMDKIDKMSGMLPDLDISMAE
                     AYQNTNDHAKAIQYYFSAYKRQPDRHNLLYSIAHAYDTAKDYKNAITTYERFLKTAPK
                     DFDVASFAVAKDEKMNAENIYYIVSFKRIKELREQLFMQSGNK"
     gene            complement(4919..7180)
                     /locus_tag="PLI30_03675"
     CDS             complement(4919..7180)
                     /locus_tag="PLI30_03675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019539454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HOI78686.1"
                     /translation="MSSGLIGYLPRIDELENPIDKYASQVISSDDQLLFTYSQSDENR
                     IFVSYNELSPYLIHALVATEDRRYYNHSGIDFIGLGRAVFKTLLLQDESSGGGSTITQ
                     QLAKLLYSPKANNKLQRVFQKPIEWVIATKLERFYTKDEIINLYLNKYDFNYNAVGIE
                     SAARTYFNKRPIDLNIQESAMLVGMLKNSSLYNPVRRENVTKERRDVVLAQMRKAGHL
                     TLQEADSIQKLPIELDFNRADHVAIPAPYYRQHLAKMMMAVKPERKNYASWQGQQFTE
                     DSLAWETDPLFGWCNKNKKPDGSNYNVYTDGLKIYSTIDSRMQKYAEQAVREHLQYLQ
                     PQFTAEKRGKKYAPYSNSVANDVEKFMQTAMKQTDRYRILKKQNWSDEKILSNFKDTP
                     VDMKVFSWKGEIDTTMTPWDSIRYHKGFLRAGFVAMNPVTGHVKAYVGGPDFAHFKYD
                     MVSSGKRQIGSTIKPYLYTLAMEEGLSPCDGMVHGPITIMAENGQPWSPRNTRGALGH
                     FVTIKWGLQNSDNWVTAYLMSLFSPYAFARMLKSFGLKTPADPVVSLALGPNDASVYE
                     MAGAYTAFVNRGIRVEPLLVTRIEDSYGNVVANFVPRMQEIFSETTSYKMLDMLKAVV
                     DGGTGGRLRRLYNLKGEMGAKTGTTNNNSDAWFMSFTPEIVASAWVGGEEPSIHFDRM
                     AYGQGATAALPIHGLFYQRVYANPELKYSDNGKFDIPADFQPCYDTQRYSSDFYLDED
                     PIEQSEGIDDLFN"
     gene            complement(7418..8737)
                     /locus_tag="PLI30_03680"
     CDS             complement(7418..8737)
                     /locus_tag="PLI30_03680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016661142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HOI78687.1"
                     /translation="MISKENKKLAQWAMDCALKNGCQEAKVSLYSGSNTSFELRDAQM
                     DKLQQASESRMSLFLYVDGRYGTYSTNRLDKKELESFIRNGVESTRYLAEDEARVLPD
                     ASRYYKGGKPDLQLLDPQFSAVNPDNKVALAQAIAEEALGKDPRIISVESSYGDGDGF
                     SYQITSNGFEGETQQSWYSLSASVAVKGEGEARPSSYWYESSLYLDKLVKEGVGQKAL
                     ERTLQKIGQKKTKSGRYTMVVDPMNSGQLLRPMLSAIFGSALQQKNSFLLDKLGQKVG
                     SDKFTLLDEPHLIGASGARYFDSEGVATERRSVFDKGVLKTYYIDTYNAKKMDVDPTV
                     ANPSILVMQLGNKDLNGLISDVAHGILVTGFNGGNSNSSTGDFSYGIEGFLIEKGKLT
                     LPLSEMNVTGNMITLWNSLAETGNDPRLNSSWRIPSLVFEGVDFSGL"
     gene            complement(8734..>9753)
                     /locus_tag="PLI30_03685"
     CDS             complement(8734..>9753)
                     /locus_tag="PLI30_03685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016272500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HOI78688.1"
                     /translation="LQKLNDKIFALDKRVQKVSASLNDNTSHILFCNSEGVSYYDYRP
                     MVTLSAVCIMEENGRFENSHSARAYRKGFEFLSDDIIDVIAREAVENTSILFKAIKPK
                     GGEMPVVMGSGGSGILLHEAIGHAFEADFNRKNISIFSEQLNKKICNEHISVVDDGTI
                     PFNRGSINIDDEGTYGQKTYIVKDGILTSYLHDRISARHYGIASTGNGRRESFRNMPI
                     PRMRATYMEAGNVKESDIIASVKNGIFVDKFTNGQVQIGAGDFTFFVKSGYLIENGKL
                     TQPIKDINIIGNGPKALADITMVADNDKIDNGTWTCGKDGQSCPVTCGMPSALVSKLT
                     VGGES"
BASE COUNT         2690 a         2307 c         2211 g         2545 t
ORIGIN      
        1 taaaacaacg tatcacgatg atgaacaaaa cccaatcgcc ggcagtgaag ctggccaagt
       61 acctgactgt aattccgctg gttctgctgc tgataacctt gaacagttgt ttgagtaaag
      121 aaaagcaaac cgacgaaaaa tcgaacgatc aaacggaaga attacaaaac tcttccagta
      181 agaacgatat caaagcagat gttagtgaag gttatgtacc acctgtaacc gacagtcaag
      241 aagagatttt ttcagtagtg gaagagcaac cgctttatcc cggcggaaac gccgccatga
      301 tgaaattcct gagcaacaat ataaaatatc cggtggcagc gcaggagaaa gggactcaag
      361 gacgtgtcat caccaatttt gtggtggaaa aagacggtag cctgtccgat atccaagtaa
      421 ttcgtggtgt tgacccgtca ctggatgcgg aagctttacg tgtactaaaa accatgccta
      481 aatggaaacc tggaaagcag aagggcgaag aagtgagggt acgcttcacg cttccggtag
      541 ttttccgctt gcagtctcct cctccgccgc caccaccgcc accaacacct gtaaaggaaa
      601 gtggttccaa tgacgttttc gttgtggtag aaaaacaacc tgaattcccc ggtggaaacg
      661 ctgccatgat gaaattcctg agcaacaata taaaatatcc ggtggcagcg caggagaaag
      721 gaatacacgg acgtataatt actaattttg tggtggaaaa ggacggtagc ctcaacgaag
      781 tgaaagtggt tcgtggtgtc gatcccttgc tggacgcgga agccgttagg gttattcagt
      841 cgatgcccaa ctggaaaccg ggaatacaaa aaggagagac agtaagagta aggtatacgt
      901 tacccgttgt ttttaggttg caacaataag aggctgaaca aatggaagca cttagcgcat
      961 acatcattaa agttaacata gcactgatat ttttttattt gctgtatgtt attttcttga
     1021 aaaaagacac attttttgct ttcaggcgct attttatttt agcagcgaca gtgttttcat
     1081 ttgtttaccc gtttattacc gtatctgcct gggaaaatat aatttcattt gaaaaaccac
     1141 agaaatacac tactcaggta acggtggaag atcctgcttt tgccgttgtt ttggcagacg
     1201 aagttcaaac agcggaacct gtcaacacga ttaacattaa ctggtggcag gttttacttc
     1261 tcttattgtc cgcaggagta gtcttcttct tagctcgttt tacctggcaa ctggtcagta
     1321 ttttacagat aaaacacaaa agcatcagga agaatattga tgggatggag gtattcgact
     1381 tatacgatga aataacaccg ttttctttct tccgatggat cttcatccac ctcgactcac
     1441 attccgacac cgaaattaaa cagatacttc tgcacgaaca aacgcatgca cgccaatggc
     1501 attctgttga tattattctc atggagttgc tttgcgtatt tttatggtgg aaccccatca
     1561 tctggctgat gaagagggaa acagccatta acctggaaca tctggccgac aacggtgttt
     1621 tgcgaaaagg agtaaacagc cgcgattatc aatattatct tctacgcctt acctaccacc
     1681 aaaatgctac ccggattgta aacaatttta atgtatcact tttaaaacaa cgtatcacga
     1741 tgatgaacaa aaccaaatcg ccggcacgaa agctggtaaa atacctgatt gccctgccat
     1801 tggcgcttct tttaatggca ggcaacagtg tttacgccca aaaccaaaga gagagtgaaa
     1861 tctttacaga agtagagaaa tatcctgaat tcccgggagg gaaccaggca tttatgaagt
     1921 atcttgtcga aagcatcaag tatccggtta aagctcagga aaacaacgaa caaggcagag
     1981 ttatcaccaa ctttgtggta gaaaaggacg gaagtatttc caatattaaa gtcatccgtg
     2041 gagtaactgc cgcgctggat gccgaagccg tgaggttgct gaactccatg cctaattggc
     2101 aatctggcga acatcagggt aaacccgcca gggtacggtt taccttgccg attgtcttca
     2161 ggttacaagg cggcagcgca gggaagagcc agagccctgt aacgccggtt tctgcagatg
     2221 tcaatgaaaa aggaaaaatc ctggacgaag tggtcgttgt aggctatggg ccaacccatc
     2281 gagaatcaat accttcccca caggaatatg ccgaatcaaa cggcaacgaa ctctttattg
     2341 ttgtggaagt ccagccgcaa tatcccggtg gagatataac catgaacaaa ttcatttctg
     2401 acaacttaca atatccaaaa attgcacaag aaaatggaat tcaaggacga gtaatcactc
     2461 agtttattgt tgaaaaagac ggaagccttt ccgatatcca ggttgttcgt ggtgtagatc
     2521 catcgttaga caaagaagct atacgtctta ttcgcttaat gcctgagtgg atccccggaa
     2581 cgcagcgtgg aaacaaagta agggtaagat atacgttgcc tgttacattc cggttgtcgg
     2641 atgaagcacc aaccgtcgag gccgctaagg gtgttcaagg gaaactgaat gatcctgtaa
     2701 taaagattgg aagcagtgac gcaacttttg ttgtagttga agaacagccc gaatttccgg
     2761 gtggaaacgc agccatgatg aacttcatta ccgaaaacat ccgttatccg aaagaggcac
     2821 acgaaaaagg aattcaggga cgtgtaatca ccaagttcat agtgaggaaa gatggcagta
     2881 tagacagcgt acaaatcgca cggggggttg acccgttact ggatgccgag gccatccggg
     2941 tactggaatc gatgccaacg tggaagccgg gcaagcaacg cggacaaccc gtaaacgtaa
     3001 tgtttaccct gcctgtcgtt ttcaggttgg ataaaaagac ggaaaatggc attcaaaaaa
     3061 gtcaaacctc atcgaaaaat gtacaaaacc ctgaatttcc gggaggagaa aatgcttata
     3121 tgaagttctt atcggataac attaagtatc ccgttattgc acaggagaac gggattcagg
     3181 ggtttgtaga tgccgtgttc aacatcgata caaaggggaa agtcactttt gttaaattca
     3241 atagatctgt tgacccttcc ctggacaagg aagtcaaacg cgtgatagaa ttaatgcctg
     3301 actggattcc tggcaagttt aatggagcag cgacaagcat tacgagtgga gcctctttta
     3361 tttttcgcct gcaaggcgat ggcgttgatg agtataaagg cccaactccc agcaacgcaa
     3421 tagttgttgt aggatacgga agttcgaaga aataaacggc atcagctgat ttgaagccgg
     3481 cagatgctca tatctcaatc tcttgttaat ctgttctcga aaaccggaga aagtgtgcag
     3541 agtagctttc tccggtttaa acataaacac ttaactcacc gcttatgaaa accgaatttg
     3601 caatttcagt tgttttgacg atggtgtcgg tttccgtatc ggcacaaacg ctaacggcac
     3661 acgatttcat gcaacaatat cgttaccggg aagccattga atggctcgaa acccagcccg
     3721 aaaccgctga aaatctgtta ttgaaagctg aaagccacga aagactctac aaccataccg
     3781 atgccctgcc cgtttacgaa cgtctcttac tgaataaccc cgatgatgta gcactgttga
     3841 tctctgccgc agaatgcgca tcgcaagccg gagacacaga atcggcacta aaatattgga
     3901 taaaagcaga cagtctatcg cctgaaaatc tgttttttca gactaaaaaa gcgatagcat
     3961 attatcggaa cggtaactgg caggaaacca tcgagagcac acaaacggtt ttccagtcag
     4021 actctattcc gctgcttctg cgaatgacgg gtgacgcgtt tcaaaacatc cacgaaccca
     4081 tttgggcaaa ctatttttac acgaaagcat acgagaaaaa tccgtccgat catctttcgc
     4141 ttagccgtat ttgcgaatat ttttacgcca tggaagaaga cggatacgac acggtagcgg
     4201 ttatgaccga aaaatacctg aacgaaatta atccgaacca aaaaaccatc ggacaactta
     4261 acggaatggc aaactattcc tccggaagat tcgacaaagc aatagatcgc ctgaaagcaa
     4321 ttgtggaaat aggcgatagc tcctacacca ccacctattt cctggggatg agcaattacg
     4381 ccaaaggttg gtactacgaa gccgtgaaat ggctcggcaa agcttatgaa cttaatcgca
     4441 atgatgtgaa tatgttctac tattacggaa cagcgctcgg gcgaacaaaa aaccgggaaa
     4501 gagccgtgga gatattacag gaaggtatgg acaagataga taagatgtca ggaatgctgc
     4561 ccgatttaga catatcgatg gcagaggcct atcaaaatac gaacgatcac gccaaggcaa
     4621 tacaatatta cttttccgca tataaacgcc agcccgaccg gcacaatctt ttgtacagca
     4681 tcgcacatgc ttacgacacg gcaaaagatt acaaaaacgc catcaccacc tacgaacgtt
     4741 tcctgaaaac tgcccccaaa gatttcgatg tcgccagctt cgccgtcgcc aaagacgaaa
     4801 aaatgaacgc agaaaacatt tattacatag tcagtttcaa gcgcataaag gaattgcggg
     4861 agcagttatt tatgcagtcg ggaaataaat aactgtaacc cggactcttt gttctccgct
     4921 aattaaacaa atcgtcaatt ccttcgctct gttctatcgg atcctcgtcc aggtagaaat
     4981 cggatgaata gcgttgtgta tcgtagcaag gctggaaatc tgcgggaata tcaaatttcc
     5041 cgttatcgga atatttcagc tcgggattgg catatacccg ctggtagaac aatccgtgaa
     5101 tgggcagtgc agcagtagct ccctgtccgt aagccatacg gtcgaagtga atggaaggtt
     5161 cctccccgcc tacccaggcc gatgctacta tctcgggtgt gaacgacata aaccacgcat
     5221 cggaattgtt gttggtcgtt ccggttttgg ctcccatctc ccctttcagg ttgtacagac
     5281 ggcgcaaccg gcctcccgta cctccgtcaa ctacggcctt gagcatgtcg agcatcttat
     5341 aggaagtcgt ttcgctgaaa atctcctgca tccggggaac aaagttggcc accacgtttc
     5401 cgtaagaatc ctcgatacgg gtaaccaaca gcggttcaac acgaatcccg cggttgacga
     5461 acgccgtgta cgcaccggcc atctcataca ccgaagcatc atttggccct agcgccagcg
     5521 agacgactgg atcggcagga gttttcaacc cgaaagattt cagcatccgg gcgaacgcgt
     5581 aaggagaaaa cagactcatc aaataggcag tcacccagtt atccgagttt tgcaagcccc
     5641 attttatggt aacaaaatgt ccaagcgctc cacgtgtgtt tcgggggctc cacggctgtc
     5701 cgttttcagc catgatggtt attggtccgt gaaccatccc gtcgcagggg ctcaatcctt
     5761 cttccatagc caacgtatag agatacggct ttatggttga accgatttgc cgtttaccgc
     5821 tacttaccat atcgtacttg aaatgcgcga agtcggggcc gcccacatac gctttcacat
     5881 gtcccgtaac cgggttcatc gctacaaatc cagccctgag aaaacctttg tggtaccgga
     5941 tggaatccca cggcgtcatc gtggtatcta tctctccctt ccaggaaaag actttcatat
     6001 ccacgggagt atctttgaaa ttactcagga ttttttcgtc actccagttt tgttttttta
     6061 aaatccggta gcggtccgtc tgcttcatgg ccgtctgcat aaatttttcg acatcgtttg
     6121 ccacgctgtt ggagtaagga gcatattttt taccccgctt ctcggccgta aactggggct
     6181 gcaggtattg taaatgttca cgaacagcct gttcggcgta tttctgcatg cgcgagtcga
     6241 tggtggaata gattttcaac ccatccgtat agacgttata atttgaaccg tctggcttct
     6301 tgtttttgtt gcaccaaccg aataacggat cggtctccca cgcgagcgaa tcttctgtaa
     6361 attgctgtcc ttgccaggat gcatagtttt tcctttcggg cttcaccgcc atcatcattt
     6421 ttgccagatg ctggcgatag tagggagcgg gaatagcaac gtggtcggca cggttgaaat
     6481 ccagttcgat ggggagtttc tgtatcgaat cggcttcctg cagggtcagg tgtccagcct
     6541 ttctcatttg ggcaagcacc acatcgcggc gctctttggt cacgttttct ctcctgaccg
     6601 ggttgtaaag cgatgaattc ttcaacatac ccacaagcat agccgactcc tgtatgttca
     6661 gatcgatggg gcgtttattg aaataagtac gtgccgctga ttcaatccct accgcattgt
     6721 agttgaaatc gtatttattg aggtaaagat tgatgatctc gtctttggta taaaaacgct
     6781 ccagtttcgt agcgatcacc cactcgatgg gtttctgaaa cacacgctga agtttattgt
     6841 tggcttttgg agaatagagc aattttgcca gctgttgggt gatggtactt cctccgccgc
     6901 tgctttcatc ctgaagcaac aatgtcttaa aaacagccct tcctaaaccg ataaaatcaa
     6961 ttcccgaatg gttataatac cgcctgtctt cagtggcaac caatgcatga ataaggtatg
     7021 gcgacaattc attataactt acaaaaatcc ggttttcgtc actttgagaa taagtaaaca
     7081 agagttggtc gtccgacgaa attacctgcg aggcatattt atcgatcgga ttttccagtt
     7141 catcgatacg cggcaggtat ccaatcaatc cggatgacat cagcaggaaa gcaacaacat
     7201 ttacagcaat gaaaagaaag aacaatcgcc atagccattt gacaatatcg tttttaggcg
     7261 gcattgtctt gtttgttttt ttattcgaaa ccgatttttt ccccatagtt aatttgaaac
     7321 cgcttggttc ttgattttga cagattttgc cgcaaaatta cacaaaaccg ttgaataagt
     7381 cgtcactatt ggtgttaaaa agaccgaaga gccctactta cagcccgcta aaatcaaccc
     7441 cttcgaagac cagcgaagga atacgccacg acgaatttaa ccgcgggtcg tttccggttt
     7501 ccgcaagtga gttccacaac gtgatcatgt ttccggtaac gttcatttcc gaaagcggca
     7561 aggtaagttt acctttttcg ataagaaaac cctcaattcc gtaagaaaaa tctccggttg
     7621 aactgttgct gttccccccg ttgaatccgg taacaaggat gccgtgggcc acatcggaaa
     7681 tcaggccgtt caggtcttta tttcccagct gcatgaccag aatggacgga ttggcgacgg
     7741 ttggatccac atccattttt ttggcgttgt aggtgtcgat gtaataggtc ttcagtactc
     7801 ctttatcgaa aacactccgg cgttctgtgg ctacaccctc actatcgaaa taccgggctc
     7861 cggacgcacc gataaggtgg ggctcatcca ggagtgtaaa cttgtcacta cccactttct
     7921 gcccgagctt atccagcagg aatgagtttt tctgctgcaa ggcggaacca aaaatagcac
     7981 tcagcatcgg acgaagcaac tgccctgaat tcatcggatc gaccaccatg gtgtatctgc
     8041 ccgacttcgt tttcttctgt ccgattttct gcagggtgcg ctccaatgct ttttggccta
     8101 ctccttcctt taccagttta tcgagataga gtgatgattc gtaccaataa gacgacggac
     8161 gggcttcgcc ctcacctttc acggctacac ttgccgagag tgagtaccac gattgttggg
     8221 tttcaccttc gaatccattg ctggttatct gataactaaa gccgtctccg tcaccatacg
     8281 acgattccac cgaaattatt cgaggatcct tgcccaaggc ttcttcggca attgcctgtg
     8341 ccaaggcaac cttattgtcc ggatttacag cgctgaactg cggatcaagc agctgcaggt
     8401 cgggctttcc tcctttgtaa taacgggagg catcgggaag tacgcgtgcc tcgtcttcag
     8461 ccaggtaccg cgtggattct accccgtttc tgataaacga ttccagttct ttcttatcca
     8521 gccggttggt ggaatatgtt ccgtaacgtc cgtctacata aaggaacaag ctcatcctgc
     8581 tttccgaggc ttgctgcagt ttatccatct gagcatcacg aagttcaaaa gatgtgtttg
     8641 accccgagta tagtgatact ttcgcttcct ggcaaccgtt cttcagggca cagtccatgg
     8701 cccattgtgc cagtttttta ttttcttttg atatcatgac tctcctccta cagttaattt
     8761 actcaccaat gccgacggca tgccacaggt aaccggacac gactgtccgt cctttccaca
     8821 agtccacgtg ccgttatcta ttttgtcgtt atcggctacc atagtaatat cagccaatgc
     8881 tttcggcccg ttaccgatga tgttaatgtc cttgatgggt tgggttaatt ttccgttttc
     8941 gataaggtac cccgatttta cgaagaacgt gaagtcaccc gcgccgatct gcacctggcc
     9001 gttggtgaat ttatcaacaa agataccgtt ctttaccgaa gcgataatgt cggactcctt
     9061 cacatttcct gcctccatgt acgttgcgcg catgcgtggt ataggcatgt tccggaacga
     9121 ttcacgacgg ccgtttccgg tggaagcgat cccataatgc ctggcgctga tgcggtcgtg
     9181 caggtagctg gtaaggatac cgtccttcac aatgtaggtt ttctgtccgt atgtaccctc
     9241 atcgtcaata tttatcgacc cgcggttaaa cgggatggtt ccgtcatcca ccacactgat
     9301 atgttcgttg catatcttct tgttgagttg ttcggagaaa atggaaatat tcttccggtt
     9361 aaaatcagct tcaaacgcgt gcccgatagc ttcgtgcaac aaaatccccg atccgccgga
     9421 ccccataacc accggcatct ctcccccttt gggtttgatg gctttgaaca agatggaagt
     9481 gttttccacg gcttctcggg cgattacatc gataatgtca tccgacagga attcgaaccc
     9541 tttccggtaa gctctcgccg aatgactgtt ctcaaacctc ccgttttctt ccataatgca
     9601 aaccgcactc aacgttacca tcgggcggta atcgtaataa ctcactcctt cggagttgca
     9661 aaacagtatg tgcgaagtgt tgtcgttgag cgatgcagat actttttgca cccgtttatc
     9721 gagtgcaaaa atcttgtcgt tcagtttttg cag
//
LOCUS       DAOSDR010000059         9705 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrimonas sp. isolate AMR_MDS_1826
            NODE_2631_length_9705_cov_3.683627, whole genome shotgun sequence.
ACCESSION   DAOSDR010000059 DAOSDR010000000
VERSION     DAOSDR010000059.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863355
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrimonas sp. (wastewater metagenome)
  ORGANISM  Petrimonas sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Dysgonomonadaceae; Petrimonas.
REFERENCE   1  (bases 1 to 9705)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9705)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:10:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,720
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 35
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9705
                     /organism="Petrimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2631_length_9705_cov_3.683627"
                     /isolate="AMR_MDS_1826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2023866"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2139)
                     /locus_tag="PLI30_03690"
     CDS             complement(<1..2139)
                     /locus_tag="PLI30_03690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019537906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HOI78689.1"
                     /translation="MFKKRDILFFTFYLFTAVLTAQTETNLYRQADKTELNPWVDSVY
                     NSLSLDEKIGQLFMPVVESKSSWKTKIAGYINNQKIGGVLFSKGTLAMQAEMTNYLQS
                     IAKTPLFVALDGEWGLSMRLRDAPRFPRNLITGAIHDEETLKQYGKEVARQCREMGIH
                     INFAPSIDVHSNPDNPVIGSRSFGELPANVARQGIAFARGMEENGVMAVAKHFPGHGD
                     TSEDSHRTLPTISHDLARLDSVELHPFQQYVNAGLSGMMLGHLNVPVLNTNGLPASLS
                     INVGQKLLKDRMGFTGLTFTDGMAMKGVSNQPDMSVKALLAGNDVILGVINQEKEFQQ
                     VKQAVEKGVISPALLEEKVRKILTYKYILGVHHVKPIDIRTLNRNIHAPESEWTQRKI
                     YDKAITLVKNNDSILPVRALDKNRIAAVAIGTPEQNTFQKWLKKYAGVNTFQVITVDS
                     IAPLSDKLKEFDIVLISVHSVPAGGSTALQQMIKNAAKSVLVLFDSPYTLDNLKPATD
                     LASSVVVAYDNTDFAQMSAAQAVFGGIGFSGKLPVSAGGFEVNSGIGTQKTRLSYSLP
                     EEVGMLSESLQDVEKIALEGIRQRAYPGCYVMVVKDGTVIYNKGFGNMEYSSGAKVDA
                     ETVYDLASITKAAATVPAIMKMYDEKKIRLQDNIGKFVPETRGTNKANLTIRDALFHE
                     TGIVAFIPYYTTAIDPNSFNGSLFGRKSTLH"
     gene            complement(2203..3093)
                     /locus_tag="PLI30_03695"
     CDS             complement(2203..3093)
                     /locus_tag="PLI30_03695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019537905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HOI78690.1"
                     /translation="MRLPLFSMEGSPLLNNPQHLMNKKYFFLLILTVFMCGTVAFGQK
                     SIVILHTNDTHSRIEPIPESDRIAGNKGGVVRRMNYIEQVRKENKNVLLFDAGDFLQG
                     TPYFNLFKGEVETEAMNMMRYDAVTLGNHEFDYGLEALEKVVRRAKFPIISSNYDFSG
                     TPLNNLIKPYLIFKKDGVKIGVIAINIQPKGLIASGNYDGMKFLQPERVANELALKLK
                     TTDRCDMVICLSHLGYTADKRLVEQTRNIDIVIGGHSHTNMKTPDMLKNIDNKDVMVF
                     QTAGRGIYVGRIDVELEKVN"
     gene            complement(3047..3802)
                     /locus_tag="PLI30_03700"
     CDS             complement(3047..3802)
                     /locus_tag="PLI30_03700"
                     /inference="COORDINATES: protein motif:HMM:NF014871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0009166 - nucleotide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-nucleotidase C-terminal domain-containing
                     protein"
                     /protein_id="HOI78691.1"
                     /translation="MNRQLIILVLAAILLISCKSYQYRISSMNGTVVEMNSRFDANPN
                     KKMEALVRNYKTELDREMNEVIGTSAQLLDYGRPESLLTNFTSDVMKIYGDEHISGGA
                     DVAIMNVNGHRANMPKGKITVGNLYEIYSFDNAITFLELKGTDLKKIFDSYAWIGGAG
                     ISSNVRLAIKDRKVQSVTVDGKPIDENKVYTVSTLDYLADGNNDMDALRNALKTTNTG
                     ITLRDVMIDYVKEQTRQGNEITSLLDGRITVIE"
     gene            4024..4374
                     /gene="rplS"
                     /locus_tag="PLI30_03705"
     CDS             4024..4374
                     /gene="rplS"
                     /locus_tag="PLI30_03705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009859510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HOI78692.1"
                     /translation="MDLIKIAEESFASGKKELPKFKSGDTITVAYRIVEGNKERIQQY
                     RGVVIRISGDGDNKRFTVRKMSDNIGVERIFPINSPFIDSITVNSEGKVRRTKLYYLR
                     QLRGKAARIKKKTF"
     gene            4447..5169
                     /gene="recO"
                     /locus_tag="PLI30_03710"
     CDS             4447..5169
                     /gene="recO"
                     /locus_tag="PLI30_03710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018074785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HOI78693.1"
                     /translation="MHYKTEGIVLSNAGYNDKYSITHVFTRDFGRVSYLLPKIRGRRS
                     KINPALFSPLSILNLEVEHLPLREIQRLKEADRQTLLYDIGVNLTKVSLTFFLSEFLS
                     KVLRETDNNELLFDYLKQAVEVLEETKTGLANFHLTFVFGLTRFLGVYPNLENYTRGC
                     CFDMLNGEFTCRTPDHAYYLRERESAFLNRLSRINFSNMHLFILSRNDRNLIIDRMLT
                     YYRLHLYDFQPIKSLDVLRELS"
     gene            complement(5127..5813)
                     /locus_tag="PLI30_03715"
     CDS             complement(5127..5813)
                     /locus_tag="PLI30_03715"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019537901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004788 - thiamine diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0006772 - thiamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HOI78694.1"
                     /translation="MRSGNKDDGSTAVIVANGRYPHHPVPLSVIKNAPYIVCCDGAAN
                     HFIEAGGYPDAIVGDCDSISEENRVRFADKLFPDKEQETNDLTKSVLFCVENGRRNII
                     IVGGTGIREDHSIGNISLLTDYEAMANVKMVTNYGIFTPISSKTTFNSFAGEKVSLFS
                     IGQKPITTDGLKYPIENRILTNWWQGTLNESLGDRFTVDTCGRVLVFQVFDSEKDQDN
                     SRSTSSDLIG"
     gene            complement(5857..6591)
                     /gene="ric"
                     /locus_tag="PLI30_03720"
     CDS             complement(5857..6591)
                     /gene="ric"
                     /locus_tag="PLI30_03720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019537900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster repair di-iron protein"
                     /protein_id="HOI78695.1"
                     /translation="MLINSESIIGKIVAENYKTAAVFKSYNIDFCCNGNRSIAAASKE
                     KDIDEKVILQELSEVLNKKEDSDIDFKSWSIDLLADYIEKKHHRYVFAKIPEITAYLA
                     KITDVHGNIHPELHEINELFSQSAEALSAHMKKEENILFPLIREMVDKRTTGEKFKKP
                     PFGSIENPIKTMHAEHDNEGVRFRRISELSRNYETPQDGCNTYRVTLNLLKEFEDDLH
                     RHIHLENNILFPAAIEFERVIFSTHT"
     gene            complement(6662..8887)
                     /locus_tag="PLI30_03725"
     CDS             complement(6662..8887)
                     /locus_tag="PLI30_03725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019537899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric-oxide reductase large subunit"
                     /protein_id="HOI78696.1"
                     /translation="MNHKKLWIALSLVVGISFAVLLFYGNQIYQKAPPVPEQVVDESG
                     NVLFTGSNIKDGQNVWQSMGGQEVGTVWGHGAYVAPDWTADYLHREAQYLLNKWAGGD
                     FESQSSEQKALLERRLQIFLRKNTYDEATKTLTLAAERIEAFEHNKAHYTSLFMNDPA
                     LDKLRSDYAIPKNAIKDESRMEKMAQFFFWASWACVTERPGESITYTHNWPNDAQIGN
                     VPTGDLVIWTGFSIIMLLIGIGILVFVQARTQQEEVLKPVNDPLMNQVITPSMRATKK
                     YFWIVNILILAQVMLGILTAHYGVEGDALYGIDIASFLPYSITRTWHVQIAIFWIASA
                     WLATGLYIAPSLSGRDPKFQKLGVNVLFVALLIVVAGSLIGQWFGVMQKLGLVENFWF
                     GHQGYEYVDLGRFWQLLLVIGLFLWLTLMIRPIVPIIKKGTSERGLLILFLISCFAIA
                     FFYAAGLMWGRTTNLAIAEYWRWWVVHLWVEGFFEVFATVVAAFLFTRMGLLRIKSAT
                     NNVLFATIIFLSGGILGTFHHLYFTGTPTGVMALGATFSALEVVPLVLIGFEAFHNYR
                     MSKSTEWLADYKWPIYFLLSVAFWNFLGAGIFGFIINPPIALYYMQGLNTTPVHGHAA
                     LFGVYGMLGIGLMLFVLRSMYRKQKWNDKLIKFTFWTLNAGLLLMVVVSLLPVGLMQT
                     FASVNHGMWYARSAEFMQQPVVNVFKWSRIIGDTVFGIGTLTLFLFVYQLTLKKNKST
                     N"
     gene            complement(9001..9435)
                     /locus_tag="PLI30_03730"
     CDS             complement(9001..9435)
                     /locus_tag="PLI30_03730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019537897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HOI78697.1"
                     /translation="MFSKACKYGIKALVYIAIQSIEGKRVTLGEISKKTDTPEAFTAK
                     ILGTLSKNGLVSSFTGPNGGFEINPDKMKAVKMSDIVSVIDGNEIIWQCPLGLTGCND
                     ENPCPVHHQFAEIRTKLTAMLVSTTVYDMATELKSNIQVLLR"
     gene            complement(9525..>9705)
                     /locus_tag="PLI30_03735"
     CDS             complement(9525..>9705)
                     /locus_tag="PLI30_03735"
                     /inference="COORDINATES: protein motif:HMM:NF014997.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HOI78698.1"
                     /translation="NEKNCIEVIGWLIAGGVFYITGAIIYATARREFVHAVFHLFVLF
                     GLASHIIGAGIIPLD"
BASE COUNT         2450 a         2248 c         2167 g         2840 t
ORIGIN      
        1 gtggagtgtc gactttcttc cgaacagcga cccgttaaaa ctgttcgggt caatggccgt
       61 tgtgtaatag gggataaaag caactatgcc cgtttcgtga aaaagcgcgt cgcggatggt
      121 taggtttgct ttgttggttc cccgagtttc gggtacaaat ttcccgatat tgtcttgcag
      181 ccggatcttt ttctcatcgt acatcttcat gatcgccgga acagtggctg ctgccttggt
      241 tatcgaggcc aggtcataaa cggtttcggc gtcaactttt gccccggaag agtattccat
      301 attgccgaat cctttgttgt aaataactgt cccgtctttt accaccatta cgtaacatcc
      361 gggataggcc cgctggcgga tgccttcgag cgcgattttt tccacatcct gcaggctttc
      421 ggacaacatt ccgacttctt cgggaagcga gtaggagaga cgtgtctttt gggtgccgat
      481 accactgtta acttcaaatc ctcctgctga aacaggcagt ttccccgaga agccaatgcc
      541 tccgaaaacg gcttgtgctg cactcatctg ggcaaaatcg gtgttgtcgt aagccactac
      601 gactgaggat gcaaggtcag tagccggttt taaattatcg agtgtgtacg gtgaatcgaa
      661 caataccaga accgattttg cagcgttttt tatcatttgt tgaagcgcgg tcgagccgcc
      721 tgcaggaacg gagtgcacag agatcagaac gatatcgaac tctttcagtt tgtcggataa
      781 tggcgctatg ctatcgacag taattacttg aaaagtgtta accccggcat attttttcaa
      841 ccatttctga aaggtgtttt gctccggggt gccaatcgcc acggcggcaa tccggttttt
      901 atccaatgcc cttaccggca ggatagaatc gttgttttta accagtgtaa tggccttgtc
      961 atagattttt cgttgtgtcc actcactttc cggagcgtgt atgttgcggt ttaacgtacg
     1021 gatgtctatc ggtttaacgt gatgaacacc gagtatgtat ttgtatgtca gtatttttct
     1081 tactttttct tcgagcagag ccggggaaat aactcctttt tcaaccgcct gtttcacctg
     1141 ttgaaattcc ttctcctgat tgattacccc cagaatcaca tcgtttccgg ccagcagtgc
     1201 ttttacgctc atatcgggtt gattggaaac ccctttcatc gccatcccgt cggtaaaagt
     1261 aagcccggta aaacccatcc ggtccttcag cagtttctgc cccacgttta tcgataatga
     1321 tgccggtaat ccatttgtgt tcagtacggg aacgttcaga tgcccgagca tcatccccga
     1381 cagtcctgca ttgacgtatt gctggaaagg atgtaattcc accgagtcta gccgggctaa
     1441 atcgtggctt atggtcggca aggtccggtg cgaatcttcc gatgtgtcgc cgtgaccggg
     1501 aaagtgtttg gcaaccgcca tcacaccgtt ttcttccatt cccctggcaa aggcgatacc
     1561 ttgtcgggct acgttggcgg gaagttcgcc gaaggaccgt gaacctatga ccgggttgtc
     1621 gggattgcta tgcacatcga tggacggggc aaagttaatg tggataccca tttcacgaca
     1681 ttgccgggca acctcttttc catactgctt cagcgtttct tcatcgtgaa tagcaccggt
     1741 gatcaggttt cgcggaaaac gaggtgcatc cctcaggcgc atggagagtc cccactcacc
     1801 gtccagcgcc acaaaaagcg gtgttttggc tatgctttgg aggtagttgg tcatttcggc
     1861 ctgcatggcc agcgtaccct tcgaaaacaa tacccccccg attttctggt tgtttatata
     1921 acccgcaatt tttgttttcc agctgctctt cgattccacg acaggcataa aaagctggcc
     1981 gatcttttcg tccaggctca ggctgttgta tacggaatcc acccatgggt tcaattctgt
     2041 tttgtcggcc tgccgataga gattggtttc ggtttgagcg gtaagtactg cggtgaataa
     2101 ataaaatgta aaaaacagga tatctctttt tttgaacatg atcggtttat ttgtatcaac
     2161 ttcagacatt aaggttcgga gcggcaatct gtcctcaact tattaattca ctttctcgag
     2221 ttctacatca attcttccga catagattcc tcttcctgct gtttgaaaaa ccataacatc
     2281 tttgttgtcg atatttttca acatatccgg agttttcata ttggtgtggc tgtggccgcc
     2341 gataacaata tcaatgtttc tagtttgttc caccaatcgc ttatcggctg tgtaacccaa
     2401 gtgcgacagg cagataacca tatcgcaccg gtctgttgtc ttcagcttta aagccagttc
     2461 gtttgccacc cgttccggtt gaaggaattt cattccgtcg taattacccg aagcaatcag
     2521 tcccttgggc tgaatattga tagcgatcac accaattttc accccgtctt ttttaaatat
     2581 aaggtacggt ttgatcaaat tgttcagcgg tgtgccggaa aaatcgtaat tactgctgat
     2641 gatggggaat tttgcccggc gtacaacttt ttccaatgct tccagtccgt agtcaaattc
     2701 atgatttcca agcgtaacgg catcgtaacg catcatattc atggcttctg tctccacctc
     2761 acctttgaaa aggttaaagt agggtgtccc ctgcaaaaaa tccccggcat cgaagagtaa
     2821 aacattttta ttttcttttc gtacctgttc gatgtagttc atcctgcgga caactcctcc
     2881 cttgttcccg gcaatgcggt cagattcagg aataggctcg atgcggctgt gtgtatcgtt
     2941 tgtatgaaga atgacaatgc tcttttgccc gaaggcaacg gttccgcaca taaaaacagt
     3001 gaggatgaga aggaaaaaat attttttgtt cataaggtgt tgagggttat tcaataacgg
     3061 tgatccttcc atcgagaaga gaggtaatct cattcccctg gcgggtttgt tccttcacat
     3121 agtcgatcat cacatcgcgg agcgtgatac ccgtgtttgt ggtctttagt gcgttgcgca
     3181 aggcatccat gtcgttattg ccgtcggcaa gatagtcgag tgtggaaacc gtgtaaactt
     3241 tgttctcgtc aatgggtttt ccgtctacag taaccgattg aactttccgg tccttgatcg
     3301 ccagccttac gttggaagag attcccgctc caccaatcca ggcgtaggaa tcgaaaatct
     3361 tcttcaaatc ggttcctttc agctcgagaa aggttatggc gttgtcgaaa gagtaaatct
     3421 catacaggtt tccaaccgtt attttaccct tcggcatatt ggcacgatgt ccgttaacgt
     3481 tcatgatagc tacgtcggca ccgccggaaa tatgttcatc gccgtagatt ttcatcacat
     3541 cggaagtgaa attggtgagt aaactttcgg gacggccata atccagcagc tgtgcagaag
     3601 tgccgatcac ttcattcatc tcacgatcga gctcggtttt gtagttgcgt accagggctt
     3661 ccattttttt attcggattc gcatcgaaac ggctgttcat ttcaacgacg gtgccgttca
     3721 tgctgcttat ccggtactgg tacgacttac aagaaatcag caggatagca gcaagtacga
     3781 gaatgatcag ttgtctattc attcttaatg atgggtttgt ttgatgttta ttttgtcaaa
     3841 aatacactat ttttgtcagt ttaacaacgt gtgtaaccta tttttacgaa taaaaggaat
     3901 cacagtattg tgatttaaat aattattgct acctttgcaa ccgctttgcg caatctctgt
     3961 cgatgtgttg tcgttatatt gcgtcctgta gagttaaata ttaacataaa gcttttataa
     4021 acaatggatt taattaaaat tgcagaagag tctttcgctt cgggaaagaa agaattgccc
     4081 aagtttaaga gtggcgacac cataacggtt gcttaccgta ttgtggaagg gaataaggag
     4141 cgtattcagc aatatcgcgg cgtggtgatc cgtatatcgg gtgacggcga caacaaacgc
     4201 tttacggttc gtaagatgtc ggacaatatc ggtgttgaac gtatcttccc gataaattct
     4261 ccgttcatcg acagcataac agtgaacagc gaaggtaagg tcaggagaac caaactgtat
     4321 tacctccgtc agttacgcgg aaaagcagcc cgcatcaaaa agaaaacgtt ttaaaagatt
     4381 tgaaaacaaa cacccggacg ctccgacttt tatcggggcg ttttttcatc catcatccat
     4441 cagcaaatgc actacaagac agagggcatt gttctgagca atgccggata caacgataag
     4501 tactccatca cgcatgtgtt tacgcgcgat tttggacgtg tctcatactt gcttcccaaa
     4561 atacgtggca gacgttcaaa aatcaaccct gcgttgtttt ccccgctttc catattgaat
     4621 ctggaagtgg aacatcttcc acttcgtgaa atccagcgct tgaaagaagc cgaccgtcag
     4681 acgttactct acgatattgg tgtaaattta accaaagttt cactgacttt ttttctctcg
     4741 gaatttttat cgaaagtgct gcgggaaacc gacaacaacg aattgctttt cgattacttg
     4801 aaacaggctg tcgaggtgct ggaagagaca aaaacaggat tggccaattt tcatctgact
     4861 tttgtctttg gccttaccag gtttctgggg gtttatccca atcttgaaaa ttatacccgg
     4921 ggatgttgct tcgacatgct gaacggggag tttacctgcc ggactccgga tcacgcctat
     4981 tacttgcgtg agcgggaaag cgcttttctt aatcgattgt cacggataaa tttctcgaac
     5041 atgcatctgt tcattctgtc gcggaacgac aggaacctga tcatcgaccg gatgttgact
     5101 tattaccggc tgcatttgta cgattttcaa ccgatcaaat cactggatgt gcttcgtgag
     5161 ttgtcttgat ccttttcgct atcaaacacc tgaaaaacaa gcactctccc acaagtatcc
     5221 accgtaaacc tatcgcccag tgattcattc agcgtgcctt gccaccagtt ggtgagaatc
     5281 ctgttttcta tgggatattt caacccgtcg gtggtaatag gtttttgccc gatggaaaac
     5341 agggagactt tttcacccgc aaaactattg aaagtggttt ttgaactgat gggtgtaaaa
     5401 ataccgtagt tggtaaccat cttcacgttt gccatcgcct cataatctgt aagaagcgag
     5461 atgtttccga tgctgtggtc ctcacgtatc ccggttccgc ccacaataat gatgtttctc
     5521 cttccgttct ccacacaaaa cagaaccgat ttggtcaggt cattggtctc ctgttctttg
     5581 tcgggaaata atttatccgc gaagcggacc ctgttctcct cggagatgga gtcgcagtcg
     5641 cccacaatag catcgggata tccgccggct tcgataaaat ggttagccgc cccatcgcag
     5701 caaacgatgt agggtgcgtt ctttattacc gatagcggca cagggtgatg gggatacctt
     5761 ccgtttgcaa caattactgc tgttgagccg tcatctttgt ttcctgatct cattttacgg
     5821 gttctttgat acaaagataa aaacagtaat cgaattttac gtgtgagtgg agaaaataac
     5881 gcgttcaaac tctatcgcag cagggaacag aatattgttt tccaggtgga tgtgcctgtg
     5941 gaggtcatct tcgaattctt tgagcaaatt cagggttacc ctgtaggtat tgcagccgtc
     6001 ttgaggtgtt tcgtagttcc tgctcagttc actgattctt ctgaaacgaa cgccttcatt
     6061 gtcgtgctct gcatgcatcg ttttaattgg gttttcaatg cttccgaaag gaggcttttt
     6121 aaatttttcg cccgttgttc ttttatccac catttcccgg attaaaggga acaagatgtt
     6181 ttcttctttt ttcatgtgtg ccgataacgc ttctgcagat tgagaaaaca actcgtttat
     6241 ttcatgtagt tcgggatgta tattaccgtg aacatccgta atctttgcca ggtaggccgt
     6301 tatttcagga atttttgcaa acacatacct gtgatgtttc ttctcgatat aatcggcgag
     6361 caggtcaatc gaccacgatt taaaatcaat gtccgaatct tctttcttgt tcagtacttc
     6421 cgataactct tgcagaatta ctttttcgtc gatgtccttt tctttactgg cagccgcaat
     6481 gctacggttt ccgttgcaac agaaatcgat gttgtatgat ttaaagacag ctgccgtttt
     6541 gtagttttca gctacaattt ttccaataat actttctgaa ttgatgagca tgttttttta
     6601 ttttatataa aggattgcac atccgttaaa caaccggacg tgcaatccgt gaatgaatgg
     6661 actaatttgt tgatttattt tttttcaggg ttaattgata aacaaacagg aagagcgtaa
     6721 gggttcctat gccgaacacg gtgtctccga tgatcctcga ccatttgaat acatttacca
     6781 ccggttgttg cataaattca gccgaacgtg cataccacat tccgtggttt acactggcaa
     6841 acgtttgcat taatccaacg ggcagtaagc tgacgaccac cattaaaagc aagccggcgt
     6901 tcagtgtcca gaacgtgaat ttaatgagtt tgtcgttcca tttttgtttg cggtacatgc
     6961 tgcggagcac aaacagcatc aaccctattc ccaacatgcc gtacacaccg aaaagtgctg
     7021 cgtgtccgtg tacgggagtg gtgtttaacc cctgcatata atacaatgcg atgggcgggt
     7081 tgatgataaa tccaaaaata ccagccccca ggaagttcca gaaggctacc gataacagga
     7141 aatagatggg ccacttgtaa tccgccagcc attcggttga tttactcatc cggtaattgt
     7201 ggaatgcctc aaacccgatc aacaccagcg gaacaacctc caaggcgcta aatgtcgctc
     7261 ccagtgccat gacacctgtg ggtgttcccg tgaagtaaag gtggtggaat gttcccagga
     7321 taccgcccga aaggaagata atggtggcga acagcacgtt gttggttgcc gattttatcc
     7381 tcagtaaccc catccgggta aacaagaacg cggcaactac cgttgcaaat acttcgaaga
     7441 agccttcaac ccacaaatgc acaacccacc agcgccagta ttcagcaatg gccaagttgg
     7501 tggtgcgccc ccacatcagg cctgcagcgt agaaaaatgc gatagcaaag caggagatca
     7561 ggaagagtat gagcaatccc ctttcactgg ttcctttttt gatgataggg acaatgggcc
     7621 ggatcatcag tgtaagccac aggaataatc ctatgaccag cagtaactgc cagaatctcc
     7681 ccaggtcgac atattcataa ccctggtgtc cgaaccagaa gttctcgacc agtcccagtt
     7741 tttgcatcac gccaaaccat tgtccgataa gtgaaccggc aacaacaatg agcaacgcca
     7801 cgaaaagcac gttcacgccg agcttttgaa atttaggatc cctgccgctt aacgacggag
     7861 caatatagag tccggtggct aaccaggcgc ttgcaatcca gaaaatagca atctgaacgt
     7921 gccatgtgcg tgtgatggag tagggaagga agctggcaat atcaatgccg tagagcgcat
     7981 cgccttccac gccgtagtgt gcggtgagaa tccccagcat tacctgtgcc aggatcagga
     8041 tgtttacgat ccagaaatat ttctttgtcg ctcgcatcga cggggtaatc acctggttca
     8101 ttaacgggtc attcaccggc ttcaacactt cctcctgttg tgtacgggcc tgtacgaaaa
     8161 caagaatacc aattccaatt aacaacatga tgatgctgaa gcctgtccag attaccaggt
     8221 cgccggtcgg tacgttcccg atctgagcat cgttgggcca gttgtgggta taggttatgc
     8281 tttccccggg cctttctgtt acgcatgccc acgatgccca aaagaaaaat tgtgccattt
     8341 tctccatcct cgactcgtcc ttgatggcat tcttcggtat ggcgtaatcg gaacgcaatt
     8401 tatccagcgc cgggtcattc ataaacaagg atgtataatg agccttgttg tgctcaaacg
     8461 cctctatcct ttctgcggca agggtcagcg tttttgttgc ttcgtcgtag gtgtttttgc
     8521 gtaaaaatat ctgaagacgc ctttccagca gagctttttg ttcggagctc tgagactcaa
     8581 aatcgcctcc ggcccatttg ttcagcaaat attgtgcttc ccggtgcagg tagtcggctg
     8641 tccagtccgg tgcaacatag gcaccgtgcc cccaaaccgt tcctacttcc tgtccaccca
     8701 tgctttgcca aacgttctgc ccgtctttta tgttacttcc ggtaaaaaga acgtttcccg
     8761 attcatccac cacctgttcg ggtaccggtg gcgctttctg gtagatctga ttgccgtaaa
     8821 acaataatac ggcaaatgaa attcctacta ccagtgagag tgcaatccaa agttttttgt
     8881 gattcataat taaacacgtt taatgattag taattggatt aaattgattt tatagcgtat
     8941 tgaaaatcaa ttgtttgata tatttcggat aaaagtgtcg gaaattaatt taaaaaaaac
     9001 ttaccttaat aaaacctgta tgttcgattt taactccgtt gccatatcgt aaacggttgt
     9061 agatactaac atggcagtta atttcgttct aatttcggca aattgatgat gcacggggca
     9121 gggattttca tcgttgcaac cggtgagccc caatggacat tgccaaatga tctcatttcc
     9181 atcaataacc gatacgatat cgctcatctt caccgccttc attttgtcgg ggttgatttc
     9241 aaaaccaccg ttgggtccgg taaaagaaga aactaatccg tttttcgata atgttcccag
     9301 aatttttgcg gtaaaggctt cgggcgtatc ggtctttttt gaaatctctc ccaaggtaac
     9361 tctttttcct tcaatggatt gaatagcgat ataaacgagt gccttgatgc cgtatttaca
     9421 agcttttgag aacattcatg attctttgcc tgcaaatata tgttatattt ggtacaaaca
     9481 aaaaaataat accgtttttt ttcttcccca taaaaaatta cgccttaatc aagagggata
     9541 atgccggcac cgataatgtg gcttgccagt ccaaaaagca caaacaggtg aaaaactgcg
     9601 tgaacaaatt cacgcctggc tgtagcgtag atgatggcgc cggtaatgta aaagacgcct
     9661 ccggctataa gccacccaat aacttcgatg cagttttttt cgttt
//
LOCUS       DAOSDR010000060         9613 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrimonas sp. isolate AMR_MDS_1826
            NODE_2661_length_9613_cov_4.142812, whole genome shotgun sequence.
ACCESSION   DAOSDR010000060 DAOSDR010000000
VERSION     DAOSDR010000060.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863355
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrimonas sp. (wastewater metagenome)
  ORGANISM  Petrimonas sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Dysgonomonadaceae; Petrimonas.
REFERENCE   1  (bases 1 to 9613)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9613)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:10:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,720
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 35
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9613
                     /organism="Petrimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2661_length_9613_cov_4.142812"
                     /isolate="AMR_MDS_1826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2023866"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..744
                     /locus_tag="PLI30_03740"
     CDS             <1..744
                     /locus_tag="PLI30_03740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008155745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HOI78699.1"
                     /translation="GLISENKGQNYAVLSDILGDEDHLGDMDFKVCGTKDGITATQMD
                     IKVDGLSYEILEKALAQAKAGREHIMGKMMETLSEPRADLKPHAPRIEVIEIPKDFIG
                     AVIGPGGKIIQGIQEETGAIITIEEVDNKGRVEVSASNKDSIDAAMRKIKGIVAVPEV
                     GEVYEATVRSVMPYGAFCEFLPGKDGLLHISEIDWKRLEKVEDAGIKEGDKIAVKLID
                     IDPKTGKFKLSRKVLLPRPPRPEQPRTEE"
     gene            891..1088
                     /locus_tag="PLI30_03745"
     CDS             891..1088
                     /locus_tag="PLI30_03745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008773631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI78700.1"
                     /translation="MESTTLKCGDCPLRAKYDRKPKSLAGRFWRWHINFCPGWKAYFT
                     SLAPEEKAQLREKYSFKKYTG"
     gene            1287..2870
                     /locus_tag="PLI30_03750"
     CDS             1287..2870
                     /locus_tag="PLI30_03750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015531649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HOI78701.1"
                     /translation="MNKQLLLGAEAIAQAALDAGISGVYAYPGTPSTEITQFIQQSPQ
                     ARESGVRSKWSANEKTAYEASLGMSYAGKRALVCMKHVGLNVAADAFMNSAITGVHGG
                     LVVVVADDPSMHSSQNEQDSRVYGKFAMIPVLEPSNQQETYDIVRYAFDLSEEVKLPV
                     LLRIPTRLSHSRAVVSGGEAMPQNQLAPTQEKNRWILLPVNARKGYHRLLEKQAQLIN
                     FSEFSRFNQVISGTGDKAVIAFGIAYNYVMEVIRTYALDIPVLKLSQYPLPEKKISEF
                     TEKYGEVLVAEEGYPVYEELLKGFFGNEKFRGRLDGTLPRTGELSPNILSKALGVQTN
                     SEPAVPSIVAPRPPMLCQGCSHRDLFDAVVQAMSLYPQRHVFGDIGCYTLGALSPYNA
                     ISTCVDMGASITMAKGAADAGLFPAVAVIGDSTFTHSGITGLLDAVNDKSAVTVIISD
                     NGTTAMTGGQDSSGSGKYFAICKGIGVEENHIREIVPLKKNFEENVRVMKEEFEYEGV
                     SVIISQRECVQTAVKSRKK"
     gene            2867..3451
                     /locus_tag="PLI30_03755"
     CDS             2867..3451
                     /locus_tag="PLI30_03755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008154934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate oxidoreductase subunit beta"
                     /protein_id="HOI78702.1"
                     /translation="MNKNIIISGVGGQGILTMASIIDLAAMNLGLNVKQAEVHGMSQR
                     GGAVESHLRISSGEIFSDLIPKGKADLILSIEPMESLRYLPFLSPDGVIVTATEPYVN
                     IGNYPDEKELLETIANSTNHVFVNAGLLAKEAGSAKSYNVVMLGAAAPYLEIATEELE
                     KAIGMFFNRKGKEMVDLNIKAFRLGWANSLNEQK"
     gene            3448..4746
                     /locus_tag="PLI30_03760"
     CDS             3448..4746
                     /locus_tag="PLI30_03760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005676280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate--CoA ligase"
                     /protein_id="HOI78703.1"
                     /translation="MIWNPEFECADRQKLHELQSIRLREMVRRIYENVPAYRKKLQEK
                     GIEPGDIKSVDQLKHLPFTTKTDLRDNYPFGLFTLPQTDVVRIHASSGTTGKPTVVGY
                     THRDLEIWTEVVARSLTMAGVDKDDTIQVAYGYGPFTGGLGLHYGAEKVGATVIPIST
                     GNTRKQLQFMTDFHATVIACTPSYAAHLGESILKEGISPEKVKLRVGVFGAEPWTNEM
                     RAQIENLLNIKAYDIYGLSEIIGPGVSMECECQQGNHIFEDHFIPEIIDPETLETLPY
                     GEVGELVFTTVTKEAMPLLRYRTRDLTRLFIDTCDCGRTLVRMGKCLGRSDDMLIIRG
                     VNVFPSQVESVLLEMEEASPHYQLIIGRENNLDTMEIRVEINERFWSDSIRELEGIRR
                     RIDHNIKSLLGIGAAIKLVEPHSIERSEGKARRIIDNRHI"
     gene            4764..5189
                     /locus_tag="PLI30_03765"
     CDS             4764..5189
                     /locus_tag="PLI30_03765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019537793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HOI78704.1"
                     /translation="MIIKQLSVFLEDRSGRLTDLTQILAEHEINITALSLAETADYGI
                     VRMVVGKPGAAEEFLREAGFSVRLTDVVCVNMPDRPGALHEVLKILADNAINVDYMYA
                     FSNKDVALVVIRAADIDQVIEVLQKNEMQLLRQSDIYQL"
     gene            5230..6426
                     /locus_tag="PLI30_03770"
     CDS             5230..6426
                     /locus_tag="PLI30_03770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019537794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HOI78705.1"
                     /translation="MTRFAHGVSALRSSEIRDLMSLATSPDIISFSGGMPGNELFPLD
                     KIDNIYQSLTEKEKQVAMQYGPTNGLPTLLESLSQYLEKKGLPVGKNRLMMTTGSLQA
                     INILAKAFVDPGDTVLVETPSFIGALSAFRSYEANLVSVPLQGDGIDIEALRTTLNTA
                     GPKPKFLYFAPNFHNPAGIIYSEEVKHQMIELLKDQDIPLIEDDVYSDLYFYEEDVPK
                     MTNIKTMDPEGIDVCFTGSFSKILGPGLRLGWMLVPEHIYKKCELIKQSMDACSPSFS
                     QVIADKFIRNGYIYEYTENVRQEYKKRGLAMIEALEKYLPDYVSFEKPRGGFYIWLHL
                     PKGTDSSLILKKAIEKGVVFVTGKTFDPDGVKNDCIRVSFCNTATDVIQKGIPVVAEA
                     IREVCG"
     gene            complement(6436..8604)
                     /locus_tag="PLI30_03775"
     CDS             complement(6436..8604)
                     /locus_tag="PLI30_03775"
                     /inference="COORDINATES: protein motif:HMM:NF039587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5916 domain-containing protein"
                     /protein_id="HOI78706.1"
                     /translation="MIKSWLSFILMVAFAIQTFSQGTPNDTISINIRNKNFVYTVKKI
                     KGSITLDGVLDETDWQTANRVENFRLVQPVDTGFAQQKSELMLIYDDKALYMAVIFYD
                     TIPGKRIAESFRRDFAFNNNDNLLTVFDTFRDQTNGFSFGNSASGAIWDGLVSDGSVM
                     NLNWDSKVELKVKDYPDKWITEMKIPFKSIRYPSKSQTWYANFGRLDLKSNEKSVWAP
                     VPRQFPHASLAYTGVLKFDEPLPRPRTNFSLIPYLYGGYHQNFDAGENAGYRKDFGLD
                     AKIGLGTSMNLDLTYNPDFAQVEVDQQVMNIDRFELLYPEKRQFFLENSDLFANYGET
                     NTTPFFSRRIGLDAPVLGGARVSGKLGQDWRIGFMNMATEKTDAFAVRNFSVLSVQKK
                     ILSRSNLGFMMINKETLGEGSQRLFNRVAALDANLASRDNIWTGKVFYHRSFQPGNPD
                     QQYAQGASAVYNNGKVRIEFRETAVGANYLAETGYVQRRDFISFRPEISYFFVPNKRV
                     VIHGPYAEYDDYYTPGFEKLDHALDLGYKLEFRDRSTIAAGMKNYYIKLMQDFDPTHT
                     SHTFLPAGSDYSYTNIYTSFTSNNRKMLNGTVTYAKGGFFNGQYDMIDAKMVYRYQPF
                     VNFTMNATYTNIRLPEPFEHKHFWLIGPKLDITFSPKVYLTTFVQYNDQLNNTNLNIR
                     FQWRYKPVSDLFIVYTDNYFANTREVRNRALVLKMTYWWN"
     gene            complement(8618..>9613)
                     /locus_tag="PLI30_03780"
     CDS             complement(8618..>9613)
                     /locus_tag="PLI30_03780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI78707.1"
                     /translation="VFARSNFGLILVNKEYLNKPDGTLLFNRVAALDFNLASKNNEWT
                     GKVFYHRSFQPNNPDGQYAQGASVKYSKGNILISLDQAAVGDNYLAETGFVRRKNYIS
                     LSPELAYFFIPNKTVTIHGPYSKFKNYSTHQFNKLDHELDLGYKVEFRDRSSIAAGIR
                     NYYIRLMQNFDPTHISNVFLPVGSEYNYSDFYTTFTSNNRQPLNGTATYSKGGFFNGN
                     YEMIDTKMVYRFQPYVNFSMNVTYTHLHLPQPFENHQFWLVGPKLDITFTDKLFLTTF
                     MQYNEQLNNTNLNIRFQWRYKPVSDLFLVYTDNYYANTREVRNRALVLKLTYWWN"
BASE COUNT         2577 a         2070 c         2445 g         2521 t
ORIGIN      
        1 ggactgattt ccgaaaacaa aggacagaac tatgccgtcc tttccgatat cctgggcgat
       61 gaagaccacc tgggagacat ggactttaaa gtgtgtggca ctaaagacgg tattacggct
      121 actcagatgg atatcaaggt agacggatta tcgtacgaga tcctcgagaa agcccttgcc
      181 caggctaaag ccggacgcga gcacatcatg ggtaaaatga tggaaaccct ttccgaacct
      241 cgtgccgacc tgaagccgca tgcaccccgg attgaagtga tagaaatccc gaaagacttc
      301 atcggtgccg ttatcggccc gggaggaaaa atcattcagg gaatccagga agaaactggt
      361 gccatcatca ccattgagga ggtcgataac aaaggccgtg tggaagtatc ggcttctaac
      421 aaggactcta tcgatgccgc catgcgcaag ataaaaggga ttgtagctgt gcccgaagtg
      481 ggtgaagtgt acgaagcgac cgttcgttca gtgatgcctt acggtgcttt ctgtgaattc
      541 cttcccggaa aagacggatt gctgcacatc tccgagatcg attggaaacg attggaaaaa
      601 gtggaagatg ccggaataaa agaaggcgat aagattgccg taaaactgat cgacattgat
      661 cccaaaacgg gtaaattcaa actttcacgc aaggtgttgc tgccccgtcc tccaaggccg
      721 gaacaaccca ggactgaaga gtaaaaaaat agacaactct gttaaagcga gcctttccgg
      781 ttcgcttttt ttaatgatat tttttacttt tgttgtcagg ttgcactcgt ttcatctgcg
      841 ggtgtaacgc gaaacagttt cttagttgat cacaattaaa tcaaaagtca atggaatcaa
      901 caacactaaa atgcggagac tgccccttga gggcaaagta tgaccggaag cccaagtcat
      961 tggccggccg tttttggcgg tggcacatca atttttgtcc aggttggaaa gcctatttca
     1021 cctccctggc gccggaagag aaagcgcagt tgcgggagaa atatagcttt aaaaagtata
     1081 ccggttgatt accttttccg catcccggta aattcccaaa agaagagaaa aacatcggtt
     1141 ttgtttaact attttttata cctttgcaac taacttcttt tagacgggca gattattagg
     1201 cattagaccg atagacaaag ctggtatctg aaagcgaagc gatctaatgt ctagaagcgt
     1261 agtggtctga aagtctgcaa aataatatga acaaacaatt acttttaggt gcggaagcta
     1321 ttgcacaagc tgcattggat gcgggaatct cgggagttta cgcttatccg ggaacgccgt
     1381 ccaccgaaat aacacaattt attcaacaat cgccccaggc ccgggaatcg ggtgtgcgtt
     1441 cgaaatggtc tgctaacgag aaaacagctt acgaggcatc gctcggcatg tcttacgccg
     1501 gaaagcgcgc tttggtttgt atgaagcacg tggggctgaa cgtagccgca gatgcgttta
     1561 tgaattcagc cattaccgga gtccacggcg gattggtagt ggtggtggcc gacgatccgt
     1621 cgatgcactc atcacaaaac gagcaggata gccgtgtgta tgggaagttt gcgatgattc
     1681 ctgttctgga accttccaac cagcaggaga catacgatat tgtccgttac gcgttcgatt
     1741 tatcggaaga ggtgaaactt cccgtgcttc ttcggattcc tacccgattg tcacattccc
     1801 gtgcagtggt ttccggcgga gaggcaatgc cgcaaaatca acttgcgcct acccaggaga
     1861 agaacaggtg gatcctgctc cccgttaatg ccaggaaagg ttatcaccgg ttacttgaga
     1921 agcaggctca actgatcaat ttctctgaat tttccaggtt taaccaggta atctccggaa
     1981 cgggtgataa ggccgttata gcttttggga ttgcctacaa ctatgtgatg gaggttatcc
     2041 ggacgtatgc cctggatata ccggtgttga aactgtccca atatccgttg ccagagaaga
     2101 aaattagcga gtttacggaa aaatacggtg aggttcttgt tgctgaagaa ggctaccccg
     2161 tctacgaaga attgctaaaa ggctttttcg ggaacgagaa attccgggga cggctggatg
     2221 gcacgttgcc gcgtacagga gaactttccc caaacatact ctcaaaggcg ttgggtgttc
     2281 aaacgaatag tgaaccggct gttccatcca ttgtcgctcc gcggccgccg atgttgtgcc
     2341 agggatgcag ccatcgcgat cttttcgatg cggttgttca ggccatgtca ctttatccgc
     2401 aacggcacgt cttcggggac atcggatgct atacgttagg agctttgtcg ccttacaatg
     2461 ccatcagtac ctgtgtggat atgggcgcat cgataacaat ggccaaaggg gcagccgatg
     2521 ccgggttgtt tccggcagtt gccgtgattg gcgactccac cttcacgcac tcgggaatta
     2581 cgggattgct agatgccgtg aacgataaaa gtgcggttac tgtcattatc tccgacaacg
     2641 ggaccacagc catgacgggc ggacaggact catcgggaag tgggaagtat tttgctattt
     2701 gtaaggggat cggtgtggag gagaaccata tccgtgaaat tgttccgttg aaaaagaatt
     2761 tcgaggaaaa tgtaagggtg atgaaagaag aattcgaata cgaaggtgtt tcggtaatta
     2821 tttcccagcg cgaatgtgta cagacggcag tgaaaagcag aaaaaaatga ataaaaacat
     2881 catcatatcc ggcgtgggag ggcaaggaat tttaaccatg gcttccatta ttgatctggc
     2941 agctatgaat ttggggctaa atgtaaaaca ggccgaggtt catggaatga gtcaacgggg
     3001 cggagccgtg gagtcgcatc tccgcatttc ctcgggggag attttttccg accttattcc
     3061 caaggggaaa gccgacctta ttctttccat cgagccgatg gagtcgttgc ggtatcttcc
     3121 cttcctttct cccgacggcg ttattgtgac ggctacggaa ccgtatgtta atattggcaa
     3181 ttatcccgac gaaaaggaac tgctcgaaac cattgccaat tcaacgaatc acgtttttgt
     3241 aaatgcaggc ttgctggcga aagaagccgg aagtgctaaa tcgtacaacg tggttatgct
     3301 gggagctgcc gcaccttacc tggaaatagc aacagaagaa ttggaaaaag ccatcgggat
     3361 gtttttcaac cgaaaaggaa aagagatggt ggacctgaac ataaaagcct tccgccttgg
     3421 atgggcaaac tcattgaacg aacaaaaatg atttggaacc ccgaatttga atgtgccgac
     3481 aggcaaaagc tgcacgaact gcagagcata cgcctgaggg agatggtgag gcgtatctat
     3541 gaaaacgtac ccgcttaccg taaaaaatta caggaaaaag gcattgagcc tggcgatata
     3601 aaaagcgtgg atcagttaaa gcacctgcct tttaccacta aaaccgatct tcgcgataac
     3661 tatccgttcg gacttttcac attgccgcaa accgatgtgg tgcgtattca tgcatcaagc
     3721 ggaacaacag gaaagcctac ggttgtggga tatacgcacc gggatctgga gatttggacg
     3781 gaagtggttg cccgaagcct tactatggct ggggttgata aagacgatac tattcaggtt
     3841 gcttacggtt acggaccgtt tactggaggt cttggactgc attacggtgc cgaaaaagta
     3901 ggggcgacgg ttatcccgat ttctacaggt aacaccagga aacagcttca gttcatgacc
     3961 gatttccacg ctaccgttat cgcctgcaca ccctcttatg ctgctcattt gggtgaaagt
     4021 atccttaaag aaggcatatc tccggaaaag gtaaaactgc gcgtgggggt tttcggggcg
     4081 gagccgtgga caaatgaaat gcgtgctcag attgaaaatc tgctgaatat aaaagcctac
     4141 gatatatacg ggttgagcga aattatcggt ccgggagtgt ccatggaatg tgaatgtcaa
     4201 caagggaacc acattttcga agaccatttt atccccgaga ttatcgatcc ggagacattg
     4261 gaaacacttc cttacggtga agtaggggag ctcgttttca ctaccgtaac gaaagaagcg
     4321 atgccactct tgcgctaccg tacaagagat ctcacccggt tgtttattga tacgtgcgat
     4381 tgtggccgta cgcttgtgag aatgggaaaa tgcctcggaa ggtcggacga catgcttatt
     4441 atccgcggag tgaacgtctt cccgtctcaa gtagaatcgg ttctccttga gatggaggaa
     4501 gcatcaccgc attatcagct tatcattggc cgggaaaaca acctggacac tatggagata
     4561 cgggtagaaa tcaacgagcg gttctggtcc gacagtattc gtgaacttga aggtatccgc
     4621 cggcgcatag accacaacat caaaagcctg ttggggatcg gagctgccat taagttggtg
     4681 gaaccgcaca gcatagaacg ttcggaagga aaagccagaa ggattatcga taacaggcat
     4741 atatgacttt aaacttttaa tctatgatta tcaaacaatt atctgtattt ttggaagatc
     4801 gttcgggacg gcttaccgat ctcactcaga tactggcaga gcacgagatc aatatcactg
     4861 ccctgagcct ggccgagaca gctgattacg gcatcgtaag aatggtggtg gggaagcccg
     4921 gcgcagcaga ggagtttctt cgtgaagccg gcttttcggt gcgcctaacc gatgtggtct
     4981 gcgtgaatat gcccgaccgc cccggagcat tgcatgaggt gctcaagata ttggcagaca
     5041 atgcgatcaa tgtagattat atgtatgcat tttcgaataa ggatgtggct ttagttgtta
     5101 ttcgggcggc ggatatcgat caggtaatcg aggtattgca gaaaaacgag atgcaactgc
     5161 ttcgccagag cgatatttat caactttaat aaaaaagacc agacatctgc tagctaaatt
     5221 aataatatta tgacaagatt tgcacacggt gtttccgctt tacgttcgtc agaaatcagg
     5281 gacctgatga gcctggccac ttctcccgac atcatctctt tttcaggcgg aatgccgggt
     5341 aacgaacttt tcccgttgga taagatagat aatatttatc agtcgcttac cgaaaaagaa
     5401 aaacaggttg ccatgcaata cgggcccacc aacgggttgc cgacgttgct ggagtccctt
     5461 tcgcaatacc ttgaaaagaa aggattgccg gtagggaaga atcgtttgat gatgactacg
     5521 ggatcacttc aggcgatcaa catcctggca aaagcctttg tggatccggg tgatactgtt
     5581 ctggttgaaa caccttcttt tattggggca ttatctgcct ttcgatcgta tgaagccaat
     5641 cttgtttctg ttcctctcca gggagatggt atcgatatag aagcgttaag aacgacgttg
     5701 aatactgctg gcccgaagcc gaagttcctc tattttgccc ctaattttca caatcctgcg
     5761 ggtattattt attcggaaga agtgaagcat cagatgattg aattgttgaa agatcaggac
     5821 atcccgctta ttgaagacga tgtttacagc gatttgtact tctacgaaga ggatgtgccg
     5881 aaaatgacaa acataaaaac gatggatccc gagggtattg atgtctgctt tacaggttca
     5941 ttctctaaaa ttctcggtcc cggcttgcgc ttgggttgga tgcttgttcc ggaacacatt
     6001 tacaaaaagt gtgaattgat caaacaatct atggatgcct gctcgcccag tttttcacag
     6061 gtgatagccg ataaattcat ccgcaacggt tatatttatg aatatactga gaatgtgcgg
     6121 caggagtaca aaaaacgcgg gttggccatg attgaggcct tagagaagta tttgcccgat
     6181 tacgtttcgt ttgaaaagcc acgtggcggg ttttatatct ggcttcacct gcccaaagga
     6241 acggacagtt cgcttatctt gaaaaaagcc atcgaaaaag gagtggtctt cgttaccgga
     6301 aagacattcg accccgacgg ggtaaaaaat gattgtatac gtgtatcgtt ttgcaatacg
     6361 gccacagatg ttattcagaa aggaattccc gtcgttgcgg aagcgatccg ggaggtgtgc
     6421 ggataaatca atcaatcaat tccaccaata ggtcattttc agcaccagcg cacggttacg
     6481 cacttcacgc gtgtttgcaa agtaattgtc ggtatagaca ataaataaat ccgaaacggg
     6541 tttatacctc cactggaaac ggatgttcag gttggtgttg ttcaactggt cattgtattg
     6601 cacaaaagtg gtcaggtaca ctttgggtga gaatgtgatg tccagctttg ggcctatcag
     6661 ccagaaatgt ttgtgttcga agggttcggg taaacgtatg ttagtgtatg tggcattcat
     6721 ggtgaaattc acgaaaggtt gataccggta taccattttt gcatcaatca tatcgtactg
     6781 gccgttgaaa aatccccctt tggcataggt taccgttccg ttcaacattt tccggttatt
     6841 ggaggtgaac gaagtgtaaa tattggtgta actgtaatcg ctccctgccg gcaggaatgt
     6901 gtggcttgtg tgggtcggat cgaaatcctg catcagtttt atgtagtaat tcttcatgcc
     6961 tgccgcgatg gtggaccggt ctctgaattc cagcttatat ccgagatcga gtgcatggtc
     7021 caacttttcg aacccggggg tataataatc gtcatattcc gcataaggcc cgtgaataac
     7081 cactcgctta ttcggtacaa agaaatagct tatttcggga cggaaggaga taaagtcccg
     7141 gcgttggacg tatccggtct ctgccagata attggcacct acggcggttt cccgaaactc
     7201 tatcctgacc ttgccgttgt tgtatacggc cgatgctccc tgtgcgtact gttggtcggg
     7261 atttcccggc tggaaagacc ggtgatagaa tacttttcct gtccagatat tgtcgcgcga
     7321 agccaggttg gcgtccaatg ctgcaacacg gttaaacagc ctctgtgatc cctcgccaag
     7381 agtttctttg tttatcatca taaaacccag gttggagcga gagagtattt tcttctgaac
     7441 agacaacacc gagaaattcc ttacggcaaa agcatctgtt ttttccgtgg ccatgttcat
     7501 aaatcctatg cgccagtcct gcccgagttt gccgcttacc cgggcaccgc ccaataccgg
     7561 tgcatccagt cctatccttc gggagaagaa tggtgtagtg tttgtttccc cgtaattggc
     7621 aaacaggtcg ctgttctcga gaaagaactg ccgcttttcg gggtaaagca gttcaaaacg
     7681 gtcgatattc atcacctgct ggtccacttc cacctgggca aaatcgggat tgtaagtcag
     7741 gtctagattc atggaggtgc ctagtccgat cttggcgtcc agtccgaagt cttttcggta
     7801 tccggcattt tcgccggcgt caaaattttg atgatacccc ccgtagaggt acgggataag
     7861 agaaaaattt gtcctcggcc tgggtaaagg ttcatcgaat ttcaatactc ctgtataagc
     7921 cagtgaggca tgcggaaact gacgaggcac aggtgcccag accgatttct cgttcgattt
     7981 tagatcgagg cgcccgaagt tggcatacca ggtttggctt ttagacggat aacgaatgga
     8041 tttgaacggg attttcatct ccgtaatcca tttgtcggga taatctttca cctttagttc
     8101 cactttactg tcccagttca ggttcatgac cgaaccgtcc gataccagtc cgtcccatat
     8161 agctcccgac gcagaatttc cgaacgaaaa cccgttggtt tgatcgcgga atgtgtcgaa
     8221 aacagtgaga agattgtcgt tgttgttaaa tgcaaaatcc ctgcggaaag attcggcaat
     8281 tcgtttaccg ggaattgtat cgtaaaaaat gacggccatg tagagagctt tgtcgtcgta
     8341 tatgagcatc aactccgatt tttgctgcgc aaatccggta tctaccggct ggacgagacg
     8401 aaagttctcc acccgatttg ctgtctgcca atcggtttcg tccagcacgc catccagcgt
     8461 gatggaacct tttattttct tgacggtgta aacgaagttc ttattgcgaa tgtttatgga
     8521 aatggtatcg tttggagtac cctgtgagaa ggtttgaatt gcaaaagcaa ccatcagaat
     8581 aaaagataac caggatttga tcatgtttct actgttttta attccaccaa taggtcagtt
     8641 tcaataccag cgcacggttt cggacttcac gtgtattggc ataataattg tcggtataaa
     8701 ctaaaaataa gtccgataca ggtttgtaac gccattggaa acggatgttc aggttggtat
     8761 tattgagttg ttcgttatac tgcatgaacg tggtgagaaa caatttatcc gtgaaagtga
     8821 tgtcgagctt ggggccgacc agccagaatt gatgattttc aaagggctgc ggtaagtgaa
     8881 ggtgtgtgta tgtaacgttc atcgaaaaat tcacgtaggg ttgaaaccgg tacaccattt
     8941 ttgtgtcaat catttcgtaa tttccattga aaaaaccgcc tttggagtaa gtagccgttc
     9001 cgttgagtgg ttggcggttg ttggacgtga atgtggtgta gaagtcggag tagttatact
     9061 cactccccac cggtaaaaaa acgttgctta tgtgggttgg atcaaagttt tgcatcaacc
     9121 tgatgtaata attacgtatg cctgccgcaa tggacgaacg atcacggaac tcgaccttat
     9181 aacccaaatc cagttcatgg tcaagtttgt tgaattgatg agtggaataa ttcttgaatt
     9241 tggaataagg cccgtgtatg gttaccgttt tattgggaat aaaaaaatag gccaattccg
     9301 ggcttaacga aatgtaattc ttgcggcgaa cgaagcctgt ttctgcaaga taattatcac
     9361 ctactgccgc ctggtccagg ctgatgagga tattaccctt actgtatttt acggatgccc
     9421 cttgtgcgta ttgaccgtcg ggattattcg gttgaaaaga ccggtgataa aatactttac
     9481 ctgtccattc gttgtttttg ctggccaggt tgaaatccag cgccgcaaca cggttaaaga
     9541 gcagagtacc gtcgggcttg ttcaggtact ctttgttgac caggataagt ccgaagttgg
     9601 aacgggcgaa aac
//
LOCUS       DAOSDR010000061         9584 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrimonas sp. isolate AMR_MDS_1826
            NODE_2669_length_9584_cov_4.268759, whole genome shotgun sequence.
ACCESSION   DAOSDR010000061 DAOSDR010000000
VERSION     DAOSDR010000061.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863355
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrimonas sp. (wastewater metagenome)
  ORGANISM  Petrimonas sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Dysgonomonadaceae; Petrimonas.
REFERENCE   1  (bases 1 to 9584)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9584)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:10:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,720
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 35
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9584
                     /organism="Petrimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2669_length_9584_cov_4.268759"
                     /isolate="AMR_MDS_1826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2023866"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..309
                     /locus_tag="PLI30_03785"
     CDS             <1..309
                     /locus_tag="PLI30_03785"
                     /inference="COORDINATES: protein motif:HMM:NF014584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CrcB family protein"
                     /protein_id="HOI78708.1"
                     /translation="SKIVAKYAHDYFLFLGTFAANIIGCFLIGVLSGWILANQPDNQP
                     FRLLFIVGFCGGYTTFSTFAFENLRLLELGQWTLLLGYTLFSVVLGIFAAWAGMKIGG
                     "
     gene            328..1413
                     /locus_tag="PLI30_03790"
     CDS             328..1413
                     /locus_tag="PLI30_03790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019540418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HOI78709.1"
                     /translation="MNSSIKFTLTVFFTVISTSAVFAQTDSVLLREINSLKQTVNDYQ
                     HRFDVLEKQIDDLMWFRRLEDIAHVDKVRLTSTPRWKPKDPDDRFAGNKLQFYTYVFI
                     PKTVDTNRKYPLIVLPHSGIHANFSTYYAHIVRELIAQEYVVVSAEYRGSTGYGKTTY
                     ENIDYGGRENDDVLESRNYMIDNYSFVDSTRVGIIGWSHGGMIALMQILQHGDNYQCA
                     FAGVPVSDLENRLASHDESYTDYFTAPHHIGESIRENPGEYARRSPTHYARNLNKPLL
                     IYTNTNDNDVYVEEVKLMIETLKKEGKQFEYTIFEEASGGHGFDRIDSKEATDIRYGV
                     HKFLERYLNPPKPFKSAAEMRVAAYGF"
     gene            complement(1424..3205)
                     /locus_tag="PLI30_03795"
     CDS             complement(1424..3205)
                     /locus_tag="PLI30_03795"
                     /inference="COORDINATES: protein motif:HMM:NF017390.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HOI78710.1"
                     /translation="METFLYYLLRASVLMAFFYGFYRLFFGKNTFHRFNRFLLISIVM
                     LVVVLPVLRFSLLPVKKVEPIAETFAFDPSNIPVSEIVGPQPHIVIPWVPVLSVLFAA
                     GFLFAVLRYLAGLNQLIRIIRRAEKQSLADETVLCVTDKDIAPFSWMKNIVLSRKDIT
                     ADNRAIINHERAHIHLLHSYDMIFFDLFTCVFWFNPFSWLLRREIQSVHEYQADEQVL
                     TSGTDAKQYQLLLIRKSVGEHKFALANNFRQRDLHKRITMMMKNKTNKQMKWSYTAAL
                     PVLFLAMVVLSVPKLNAKVVEKEIENTSENEIMTTQMGDSIIIADNRADLTKNPLELG
                     NTRVLTKDSSDFVIAFEEKLTVSGLVKGNQGIIMGAAVVIKGTNRGTITDSQGKFVIK
                     VDKGSTLVFSMINYKSSEYTVDEAKDNLVIVLSPEELSPDEEIAVGSGQMSSLKGELR
                     GLKIREKEGGNVIGIRTPDGMQPLLIVDGKRTSEEELQGINPKEIKSIDVLKNETSTS
                     IYGEDGKNGVVLITMKKPAQHAIISTHGFKGKPLIVVDEKKMPADFELNTVNPDKIES
                     ITVLKDKSANEIYGDEGKNGVIIIKTK"
     gene            complement(3365..3730)
                     /locus_tag="PLI30_03800"
     CDS             complement(3365..3730)
                     /locus_tag="PLI30_03800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010803231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HOI78711.1"
                     /translation="MKQLTAKEEEVMRYFWENDALFVKQLVEMYPEPKPHFNTLSTYV
                     RALEEKGFLSHETFGTTYRYFAVITEEEYRNGTLRNVVRKYFDNSYLSVVSSLIKEEN
                     ISVEEVRKLLDEVEKLNEN"
     gene            complement(3944..4114)
                     /locus_tag="PLI30_03805"
     CDS             complement(3944..4114)
                     /locus_tag="PLI30_03805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007654302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Arc family DNA-binding protein"
                     /protein_id="HOI78712.1"
                     /translation="MAKKEKTTRNFALRLDSETMEAIEKWATDEFRSVNGQIQWVLDQ
                     ALKKNGRLKEKG"
     gene            complement(4117..4632)
                     /locus_tag="PLI30_03810"
     CDS             complement(4117..4632)
                     /locus_tag="PLI30_03810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI78713.1"
                     /translation="MMKKEPVEFYEVQRFRQWWTWILLLGINALFLWGCIQQLLLGKE
                     WGTNPMNDTGLIIVAAAMLLLSVAILGAKLFTYINEEGVYVKFFPFHFRYKFYDWSNL
                     HKVYVRDYKPIKEFGGWGIRFSINAGRAFTMSGNKGLQLVLMNGKKVLIGTMQAEFLA
                     EILVKLGKTKD"
     gene            complement(4663..5604)
                     /locus_tag="PLI30_03815"
     CDS             complement(4663..5604)
                     /locus_tag="PLI30_03815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007654301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HOI78714.1"
                     /translation="METKEFNFKSFKVNGFLMLFLLLLMLGGTVFCFFGGNAGVIAGC
                     FLALIWFVLLFGFVKLEPNEAVVMIFFGKYKGVLKETGFFWVNPFMSKKKLSMRARNL
                     NVNPIKVNDKVGNPILIGLVLVWKLKDTYKAMFEIDAQTMAASKPGKDGTVTYSVSKQ
                     MDAFENFVAVQSDAALRQVAGQYAYDNNVVSDAAITLRSGGDEVNVDLLNELNNRLEM
                     AGIEIVEARINYLAYAPEIAAVMLRRQQAEAIISAREKIVEGAVSMVKMALERIEDEN
                     IIEMDSDKKAAMVSNLLVVLCADESAQPVLNTGTLYQ"
     gene            5872..7992
                     /locus_tag="PLI30_03820"
     CDS             5872..7992
                     /locus_tag="PLI30_03820"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006798783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family metallopeptidase"
                     /protein_id="HOI78715.1"
                     /translation="MKKLLFITLATMALLACNNAKTGEQARENGAIFYTEFDTPFGMP
                     PFDKISFSDFKPAFLKGMEEEAGEIDSIANNTEAPTFENTIAALDNSGRLLNRVSRVF
                     FGLKGAETNDSIQALAEELSPLMSEHRDNINFNDKLFARIKTLHDDTTGMNLTTEQYR
                     LLDNYYKEFVRSGSMLGETEKNRLREINKELSSLTLKFGNNLLKETNGFKLIIDNRED
                     LAGLPEGVIAAAAETAKNEGQEGKWVFTLQKPSWLPFLQYSEKRELREKLYKAMFNRG
                     DNDNENDNKKIINDIVNLRIEKAQMLGYKTHADFILDENMAKTPANVYKLLNDVWKYA
                     LPQAKKEASELQAMVDAEDGNFKIESWDWWYYTEKLRQQKYALNEEEIKPYFKMENVR
                     EGVFEVANRLYGLNFKKLENVPVYHPEVEAYEVLDADGSHISVFYTDYFPRAGKNAGA
                     WMSSFRGQQIRNGENIRPLVFNVGNFTRPTETTPSLLTLDEVETLFHEFGHGLHGMLS
                     NVTYSGVSGTSVSRDFVELPSQIMEHWAFHPEVLKIYAKHYQTGEVIPDSLIAKIDAA
                     SKFNMGFITTEFVAAALLDMDYHTQSEKKSIDVRDFEKKSMEKIGLISEIIPRYRSTY
                     FSHIFSGGYSAGYYAYLWAEVLDADAFQPFAEKGVFDKATAASFRENILSKGNSDDPM
                     TLYKKYRGAEPNPIYLLKNRGFIQ"
     gene            8335..>9584
                     /locus_tag="PLI30_03825"
     CDS             8335..>9584
                     /locus_tag="PLI30_03825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecDF"
                     /protein_id="HOI78716.1"
                     /translation="MELKNQYLDSLSTEKVYLGYTLKQIREKEIGLGLDLKGGMNVIV
                     EMDVAQVLRALANTDDPQFNQALQETIVQNRKGSSSDFITLFRQNYERINPDGKLANI
                     FSITMSDRVAPTATNDQVITVLRQEVKSAADNSFNVLRTRIDRFGVVAPNIQRLDQAE
                     RILIEMPGITEPERVRNLLQGSANLEFWKTYNVNELGTYFNEINTRSAEYAQAVRGAA
                     ATSTDSTAVTTDSLSVDSLTLVSTAAEGEKQEINRSFVEYFAQPYFAMSGASGPIVGT
                     AAKNDTAAVNYLLQRYKDIFPADVRFKWGFKPIDQRETYFELYALKGDGTRRGPALEG
                     DVVTNAKADQGQQGSAWEVSMTMNSAGASRWATITGAEKGKSIAIVLDGYVYSAPTVN
                     DRIEGGRSSISGRFTPEEAGDLENV"
BASE COUNT         2829 a         2276 c         1928 g         2551 t
ORIGIN      
        1 tcgaaaatcg tggcgaagta tgcgcacgat tattttctat ttttagggac gttcgccgca
       61 aacattatcg ggtgtttcct gataggggtt ttgtcggggt ggatactcgc caaccaaccc
      121 gataatcaac cgttcaggtt gctttttatc gtaggattct gcggcggata cactacattt
      181 tcgacgtttg cgtttgagaa cctccggtta ctggaactcg gccagtggac gctgttgctg
      241 ggctacaccc tgttcagcgt tgtcctgggg atctttgccg cctgggcggg aatgaaaatc
      301 ggtggttaaa aaaatgaaga aaacacaatg aactcatcaa tcaaatttac cttgaccgtc
      361 ttttttacgg ttatcagtac ctcagccgtc tttgcacaga ccgattccgt cttgctgaga
      421 gagatcaact ccttgaaaca gacagtaaac gactatcagc atcgttttga tgtattggaa
      481 aagcaaatcg acgacctgat gtggttccgg cggttggaag atattgccca tgtcgacaaa
      541 gtgaggctaa ccagtactcc ccgatggaaa ccgaaagacc ctgacgaccg cttcgcaggc
      601 aacaaattac agttctatac ctatgtgttc attcccaaaa ctgtggacac caacaggaaa
      661 tacccgttaa ttgtcctgcc acacagcggg attcatgcca atttttctac ctactacgcg
      721 cacatcgtac gcgagctgat tgcccaggaa tacgtggtgg tatcggcgga ataccggggc
      781 agcacgggat acggaaaaac cacgtacgaa aacatcgatt acggcggccg tgaaaacgac
      841 gatgtactgg aaagtcggaa ctacatgatt gataactata gctttgtgga ttccacccgc
      901 gtgggtatca tcgggtggag ccacggtgga atgattgcgt tgatgcaaat cttgcagcac
      961 ggcgacaact accaatgtgc atttgccgga gtgcccgtaa gcgacttgga aaaccggctg
     1021 gcctcgcacg acgagagtta cacggattac ttcacggcac ctcaccatat cggggaaagt
     1081 atccgggaaa atcccggaga atatgcaagg cgctccccca ctcactacgc caggaacctg
     1141 aacaaaccgt tgttaattta caccaacacc aacgacaacg acgtctatgt ggaagaagta
     1201 aaattaatga tcgaaaccct gaaaaaagag ggtaaacagt ttgaatacac catctttgaa
     1261 gaagcatctg gcgggcacgg cttcgaccgt atcgacagca aggaggccac cgatatccgc
     1321 tatggcgtgc ataaatttct ggaacgttac ctgaatccgc ccaaaccgtt caaatcggcg
     1381 gcagaaatgc gtgtggcggc gtacggattt taagcttatg cttctatttc gttttaataa
     1441 ttatcacccc gttcttcccc tcgtcaccgt aaatttcatt agcggattta tcctttaata
     1501 ctgttatcga ctcaatctta tcgggattaa cggtgtttag ctcaaagtct gccggcattt
     1561 ttttttcgtc aacaacaatc aagggttttc ccttaaaccc atgagtcgaa attattgcat
     1621 gttgagccgg ctttttcatt gtaatcaaca ccacaccgtt tttcccgtct tcaccgtaaa
     1681 tggaagtaga tgtctcgttc ttcaaaacat ctatcgattt gatttccttg ggattgattc
     1741 cctgaagttc ttcttccgaa gttcgttttc catccacgat aagcaacggt tgcattccgt
     1801 ccggggtacg gattccgata acattcccac cctccttttc ccggatttta agacctcgca
     1861 attctccttt gagggaggac atctgtcctg aacccacagc gatttcctca tcgggagata
     1921 actcctcggg agataacacg ataaccagat tgtctttcgc ttcatcgacc gtatattccg
     1981 aagatttata attaatcatc gaaaacacga gcgtactgcc tttatccact tttattacaa
     2041 attttccttg tgagtcagtg atggttcccc ggtttgttcc tttaataaca acagctgccc
     2101 ccattataat accctggttg cctttcacta aaccagatac agtaagcttt tcctcaaacg
     2161 cgatgacaaa atctgaacta tccttcgtca aaactcgtgt gttccctaat tctaacggat
     2221 tttttgtcaa atctgcccga ttgtcggcaa taattatcga atcccccatt tgagtcgtca
     2281 ttatctcgtt ttcagaagtg ttttcaattt ccttttccac cactttcgca ttcagtttag
     2341 gaaccgagag gacgaccata gccagaaaca acacgggaag cgccgccgtg tagctccatt
     2401 tcatttgttt gttggttttg tttttcatca tcattgtaat tcttttgtgc aagtcgcgct
     2461 gacggaagtt gtttgccaaa gcaaacttat gttcgcccac actcttgcgg attaataaaa
     2521 gttgatattg ttttgcatcg gtgccgctgg taaggacctg ttcgtctgcc tggtattcgt
     2581 gaaccgattg aatttccctt cgcagcagcc acgagaaagg gttaaaccag aaaacacacg
     2641 tgaacagatc gaaaaaaatc atatcgtatg aatgtagcaa atgaatatgt gcccgttcgt
     2701 gattgatgat agcccgattg tctgccgtaa tgtctttgcg tgacaaaaca atgtttttca
     2761 tccaactgaa cggcgcaata tctttatcgg ttacacataa aactgtttcg tcggccaatg
     2821 actgcttctc tgccctccga ataatacgga taagctgatt cagtcccgcc aggtaacgca
     2881 aaacagcgaa aaggaatccg gcggcaaaca gaaccgacag taccggcacc catggaatta
     2941 caatatgtgg ttgcggtcca acaatctcac tcactggaat attcgaggga tcaaaggcaa
     3001 atgtttctgc gattggttct acttttttta cgggaagcaa actgaaacgc aagaccggaa
     3061 gcacgacgac cagcatcacg atcgatatca acaaaaaacg attgaagcga tgaaacgtat
     3121 tttttccgaa aaaaagcctg tagaaaccgt aaaaaaaggc catcagaacg gaggcgcgaa
     3181 gcaggtaata taaaaatgtt tccatttgtc agtatttgtg tttgtctttt ttttaagaat
     3241 ttagattgaa ttagatcgaa tgcagattga ataagattga aaaacaggca gcccactatc
     3301 aatctccatc aatctttttc aatctatcat caatattact aaaacatggc agtaattttt
     3361 attttcagtt ctcattcaac ttttccactt cgtccagcag tttcctcacc tcttccaccg
     3421 aaatattctc ctctttgatc aacgacgaga ctacgctcag gtatgaatta tcgaaatatt
     3481 tcctcaccac attccgaagc gttccgttgc gatactcctc ttccgtaatt acggcaaaat
     3541 agcggtatgt ggtcccaaat gtttcgtgag acaaaaaacc tttctcttcc agtgcccgca
     3601 cataggtgga taacgtattg aaatgcggct tcggttcggg atacatttct acgagttgct
     3661 tcacaaacaa cgcatcgttt tcccaaaaat aacgcataac ttcttcttct ttggctgtta
     3721 attgtttcat tttttacaga ggttagatgt cagacttcag accacattaa gcgtgaagga
     3781 atctactacg gcacaaatat aactattttt tttagtttta aaactaattt atatagttaa
     3841 aagaactaaa gagtatagtt aaaattaaaa gtaaaaaata aaccgactgg ttatcaatcg
     3901 gcttataaat atatgatcag ataggattaa catagctgac agattaaccc ttttccttca
     3961 acctcccgtt tttcttcaac gcctgatcca ggacccactg gatttggcca ttaacactgc
     4021 gaaactcgtc agtagcccac ttttcaatag cctccatcgt ttctgagtcc agccgcaatg
     4081 cgaagttccg ggttgttttt tcttttttag ccattttcaa tccttcgttt tgcccaattt
     4141 aaccaaaatt tcggcaagaa attcagcctg cattgttccg ataagcactt ttttgccgtt
     4201 catcagcaca agctgcaacc ccttgtttcc agacatggta aaagctcttc ccgcatttat
     4261 ggaaaaccgg ataccccagc caccgaattc ttttatgggt ttataatctc tgacatacac
     4321 cttatgcaag ttactccaat cgtaaaactt ataccggaaa tgaaacggga aaaatttcac
     4381 gtaaacacct tcttcgttaa tgtaagtaaa cagttttgca ccgaggattg ccacactcaa
     4441 caagagcatg gctgcggcaa caattattaa tccggtatcg ttcatcgggt tagttcccca
     4501 ctcctttccc agtagaagct gttgaataca accccacaaa aaaagggcgt taatccccag
     4561 caagagaatc catgtccacc actggcgaaa ccgctgtact tcgtaaaatt caaccggttc
     4621 ttttttcatc attcttgact ttatccttta accctggctc gcttactgat acaatgttcc
     4681 tgtattcaat accggctgcg ccgattcatc ggcacaaagc actactaaca ggttacttac
     4741 catggctgcc tttttatcgc tgtccatctc aatgatgttt tcgtcttcaa tgcgttcgag
     4801 cgccatcttt accatcgaca ccgctccttc aacaatcttc tcgcgtgccg agataatagc
     4861 ttcggcttgt tgacggcgaa gcatcacggc tgcaatttcg ggggcgtatg ccaggtaatt
     4921 gatgcgcgct tcaacaattt ctatacccgc catttccaac cggttgttca actcgttgag
     4981 caaatccacg ttcacctcgt cacctcccga acggagcgta attgccgcat ccgacaccac
     5041 attattgtcg taagcgtatt gcccggcaac ctggcgcaaa gcggcatcac tctgaacagc
     5101 aacaaagttt tcaaacgcat ccatttgttt actcaccgaa taagttaccg tgccgtcttt
     5161 gcccggtttg gaagccgcca ttgtctgtgc gtcaatttca aacattgcct tgtacgtatc
     5221 tttaagtttc caaaccaata ccaatcctat caaaatggga tttcccactt tatcgttgac
     5281 ttttatcgga ttcacattca ggtttctggc acgcatcgat agttttttct tggacatgaa
     5341 cggattaacc caaaagaatc cggtttcttt taaaacaccc ttgtattttc caaagaaaat
     5401 catcactact gcttcattgg gttcgagttt tacaaatccg aaaagcagaa caaaccagat
     5461 caacgccaaa aaacaaccgg caataacccc tgcattaccc ccaaaaaaac aaaaaacagt
     5521 tcccccaagc attagaagca acaaaaaaag cataagaaac ccgttgactt taaaactctt
     5581 aaaattaaat tcctttgttt ccatatcgat taatttaaaa tgatattatt tcaatatcac
     5641 aaatatatta agtattttta tacctcccaa attttcagcg ttttttttgt gtttttttaa
     5701 aatttctctc aaaaaacatg aaaaaatgac agagcatcgg gtaaaaccat cggttaaatc
     5761 cttaattaat aagcaaaaag ttataccttt gccttagaaa ctattttgaa tttttagaaa
     5821 acattcaatg cttttttaca atcgtttaaa taacaatcaa ttagtactcg aatgaaaaaa
     5881 ttattattta tcacgttagc aactatggcc ttacttgctt gtaacaacgc aaaaaccggg
     5941 gaacaggccc gggaaaacgg agctattttt tacaccgaat tcgacacacc gttcgggatg
     6001 ccaccctttg acaaaatttc cttttccgat tttaagcccg catttttaaa agggatggaa
     6061 gaggaagccg gcgaaattga ttctattgcg aacaatacgg aagcacccac ctttgaaaac
     6121 accattgcag cattggacaa cagcggacgt ttactgaaca gggtttctcg cgttttcttt
     6181 ggtttaaagg gagctgaaac caacgattct atccaggcac tggcagaaga attatccccg
     6241 ctgatgtccg aacaccggga caacatcaat tttaacgaca agctgtttgc gcgtatcaaa
     6301 acgctacatg atgacacaac gggcatgaac ctgactaccg aacagtatcg tttgctggac
     6361 aactattata aagagtttgt ccgttcggga agcatgctgg gcgaaacaga gaaaaacagg
     6421 ctccgtgaaa tcaacaagga gctttcctcc cttacgctta aattcgggaa caacctgctg
     6481 aaagagacca acggctttaa attgattatc gacaatcggg aagacctggc cggactcccc
     6541 gaaggagtaa tcgctgccgc tgcagaaact gccaaaaacg aaggacagga agggaagtgg
     6601 gttttcacac ttcaaaaacc cagctggctg ccgttcctgc agtactctga aaagcgggaa
     6661 ttaagggaga aactatataa ggccatgttc aaccgtggcg acaacgataa cgaaaacgac
     6721 aacaagaaaa tcatcaatga cattgtgaac ctgcgcatcg aaaaagcgca aatgcttggc
     6781 tacaaaaccc acgccgattt tatcctggat gaaaacatgg cgaaaacgcc ggcaaacgtc
     6841 tacaagctac tcaacgatgt atggaaatac gctttgccac aggctaagaa agaggcttcc
     6901 gaacttcagg caatggttga tgccgaagac ggaaatttta aaatagaatc gtgggactgg
     6961 tggtattaca ccgaaaaact ccgtcaacag aagtatgccc tcaacgaaga agaaattaag
     7021 ccttacttca agatggaaaa tgttcgcgaa ggcgtatttg aagtagcaaa ccgcctctac
     7081 ggtttgaatt tcaaaaagct ggaaaacgta ccggtttacc accccgaagt agaagcttat
     7141 gaggtgctgg atgccgacgg ctcccacatc tcggtattct acaccgatta ctttccacgt
     7201 gcaggtaaaa atgccggagc ctggatgagc agcttccgcg gacagcaaat ccgcaatggc
     7261 gaaaacatcc gtccgctcgt tttcaacgtg ggcaacttta cccgtcccac ggaaaccact
     7321 ccttcactgc ttacgctgga cgaagtggaa acacttttcc acgaattcgg acacggtttg
     7381 cacggaatgt tgtctaacgt gacttactca ggtgtttcag gcacaagtgt ttcacgcgat
     7441 tttgtagaac taccttcaca aatcatggag cattgggcat tccatcccga agtgttaaaa
     7501 atttacgcca aacattatca aacgggcgaa gtaattcccg attcattgat cgcaaagata
     7561 gacgcagcgt ccaaattcaa tatgggattc attaccaccg agtttgttgc ggctgcattg
     7621 ctcgacatgg actaccacac acaaagcgaa aagaagagta tcgacgttcg cgattttgag
     7681 aaaaaatcca tggagaaaat cggacttatc agcgaaatta ttccccgtta ccgcagtacc
     7741 tatttctcgc acatctttag cggaggctac tctgccggat attatgctta cctgtgggca
     7801 gaagtactgg atgccgatgc gttccagcct ttcgccgaaa aaggagtgtt cgataaagca
     7861 acagccgcct cgttccgtga aaacatttta tcaaaaggca actcggatga tccgatgaca
     7921 ctttacaaaa agtatcgggg cgcagaacca aacccgatct atttattgaa aaacagggga
     7981 tttatacaat aattaaattc tatccattaa ctcgtgggat gactttcaga aagtcatccc
     8041 cattctgcaa aaataatcca acgatattgt tcttaaaaag acaaaaaaag tgtatatttg
     8101 caggctcaaa tagaaaggca ttccagatgc ctttattttt ggcaaaaaaa atcggataaa
     8161 cacctaaaaa agaatagatt aatactcata taataaacgt aatatcgaaa tgcaaaacaa
     8221 aggatttatt aaagttttcg caatcatact cacgcttatc tgtttgttct actatctttt
     8281 accgtagtag gcagacaata caataaaaaa gccgacgcgt atgctaaagg agacttggaa
     8341 ttaaagaatc agtatctcga ttcactttct accgagaaag tttatttagg atacacatta
     8401 aaacaaattc gcgaaaaaga gatcggttta ggactcgacc tgaaaggtgg catgaacgtg
     8461 attgtggaaa tggatgttgc tcaggttctc agagcgctgg ccaacaccga cgacccgcag
     8521 ttcaaccagg cattacagga gaccattgtt caaaacagaa aaggaagttc atccgatttc
     8581 atcactcttt tccgtcaaaa ctacgaaaga ataaatcccg acggtaaact ggcaaatatc
     8641 ttcagcatca ctatgagtga ccgggtcgct ccgacagcga ccaacgacca ggtaatcact
     8701 gtcctccggc aggaagtaaa aagcgctgcc gataactcgt ttaatgtgct ccgcacacgt
     8761 atcgaccgtt tcggtgtggt tgcgcccaac attcaacgcc tcgaccaggc agaacgtatt
     8821 cttatcgaga tgcccggtat tacggaaccc gaacgtgttc gtaatcttct gcaaggaagt
     8881 gccaacctgg agttctggaa aacctacaac gtaaatgaac tcggaacgta tttcaacgaa
     8941 ataaataccc gttctgcaga atatgcgcag gccgtacgcg gagctgctgc aacatccaca
     9001 gattctacag ctgtaacaac agactcttta tcggtagatt cactgacctt ggtttccaca
     9061 gccgctgaag gagaaaagca ggaaatcaac agaagtttcg ttgaatattt tgctcaaccc
     9121 tattttgcca tgagcggtgc cagcggacct attgtgggga cggccgccaa aaacgatacg
     9181 gctgcagtta attacctgct acaacgatac aaagatattt ttcccgccga cgttcgtttc
     9241 aaatggggat ttaaacccat cgaccagcgt gagacttatt ttgaattata tgcactgaaa
     9301 ggtgacggaa ccagacgcgg gcccgccctc gaaggagatg ttgttaccaa cgcgaaagcc
     9361 gaccagggac aacaaggttc agcatgggaa gtgagcatga ccatgaactc agccggtgcc
     9421 agccgctggg caaccatcac aggagcagaa aaaggcaaat ccatcgccat tgttcttgac
     9481 ggttacgttt actctgcacc taccgtgaat gaccgcatag aaggcggacg ctcctccatt
     9541 tccggacgat ttacccccga agaagcgggt gacctggaaa atgt
//
LOCUS       DAOSDR010000062         9529 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrimonas sp. isolate AMR_MDS_1826
            NODE_2683_length_9529_cov_4.212371, whole genome shotgun sequence.
ACCESSION   DAOSDR010000062 DAOSDR010000000
VERSION     DAOSDR010000062.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863355
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrimonas sp. (wastewater metagenome)
  ORGANISM  Petrimonas sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Dysgonomonadaceae; Petrimonas.
REFERENCE   1  (bases 1 to 9529)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9529)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:10:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,720
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 35
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9529
                     /organism="Petrimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2683_length_9529_cov_4.212371"
                     /isolate="AMR_MDS_1826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2023866"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..728)
                     /locus_tag="PLI30_03830"
     CDS             complement(<1..728)
                     /locus_tag="PLI30_03830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008154974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOI78717.1"
                     /translation="MKQRKLKMGMIGGGSDAFIGAIHRRAAFMDNYIELVCGCFSVNP
                     EISRSSGREYFLPDHRIYDSYLEMFEKEMELPAGERMDFVTIVTPNKWHFEPAMMALQ
                     RGFHVVIDKPMTFSLEEALKLKEKVEETGLVLALTHVYSGYPAVKEAKARIANGELGK
                     LRKVYVEYTQGWLSQRIELQGGNNAGWRTNPKTTGKAGCMGDIGTHAWHLAEYVTGLK
                     VLEVCADLQTYVEGRLVDDDGASLL"
     gene            complement(803..2026)
                     /locus_tag="PLI30_03835"
     CDS             complement(803..2026)
                     /locus_tag="PLI30_03835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019539864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOI78718.1"
                     /translation="MNKNKKIQLSAMMFFQFFIQGSWYVTMGTYLGRTLDFSGVQIGL
                     AYSTAAIAAIISPVIVGMVADRFFPAKRVLAFLNIVGAILLFSLTRIESFSLFFPVLL
                     LYSICFMPTMSLTNSISFANLRDPAREFSKIRLFGSFGWICSGLLISALKIEPYSTPF
                     LIGAGISLLAGVYALTLPHNAPVRTMEKADVRQLLGFDAFKLTRDRSFLVLLVFSALI
                     CIPLAFYDSFTNLFITDVGIENAAAAMSLGQVAEVIFLFIFPIFFLKLRYKGSIVAAI
                     AAWILMYGLFTLGASTGQNGFIYAVLPLHGFCFTFFFVAGQLFVDEKAPSSLRNSAQG
                     LIAFATYGVGKYLGTLIAGNVVDLYSEQGRYDWISIWTVPFLMAIVFLAGFLFLFRED
                     VKKRHNIKETIPGTT"
     gene            complement(2073..3662)
                     /locus_tag="PLI30_03840"
     CDS             complement(2073..3662)
                     /locus_tag="PLI30_03840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010266199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HOI78719.1"
                     /translation="MQKSLLFSLFLVTETLTFSLLAQKRPSQRPNILFCIADDASYPH
                     FGANGSKWVNTPGFDRMAREGILFSNCYTPNAKSAPSRACVLTGLYSWQLKEAGNHIP
                     QFPSDIKVVTEVLKENGYQIAFSGKGWAPGTAHTKEGEQRELTGTPFQKYTLIPPTPY
                     IGKNDYTENFKDFLNRASEDKPWFFWMGFTEPHREYEYGSGEKFGGKSKEMIDKVPMY
                     WPDNDVVRTDMLDYAFEIEYLDKHLAAIISELERRDLLDNTLIIFTSDNGMPFPRSKA
                     NSYEISNHMPLTIMWKNGIINPGRVVEDYINFVDFTPTFLEVSKTNPIKHGMIIPTGK
                     SLTEIFKNKKAGIVTHSRNFTLLGRERHDYGRRGNQGYPTRGIIQDNLLYILNLKPDL
                     LPAGESETGYLDCDGSPTKTTILNMKRSGQNNRYFQQSFGKREPEELYNLSLDKDCII
                     NLIDNPTYSKQKERLKNKLFEQLRAQNDPRMFNNGNVFDSYPFSSENAWDFWERVVSG
                     KIMEPWKQTNWVNPTDYESYK"
     gene            complement(3945..6941)
                     /locus_tag="PLI30_03845"
     CDS             complement(3945..6941)
                     /locus_tag="PLI30_03845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005649807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6067 family protein"
                     /protein_id="HOI78720.1"
                     /translation="MKFIIICAFSALLLSCSSTQYNNGEDKYQQFAPDGIPFVIADTI
                     WDVDGRGNHRAIVHVDKQEHEAVIVNLPWRRSDLRPETKKIIVTDASNGNEIKDVSVI
                     EQTAEKGTIAFKPVENESDYIVYYLPYKYRKGSGDARYGKPWNDYTTPEYETDKNWEE
                     NVLKNLKNIPNATVTRFESRTKFDFLTSMGVIATENEKYKLLNGNKSNFLTFPEDRAF
                     PIKLTTIPYRWVKNGPSLRFEGDALRNEYYAWQLGLWAIEKELRDVKVTFTDLIHESN
                     KYNIPKEQITCFNLEGVNWDGSPLKFDVHVPANKVQALWFGVQIPNDAKVGRYKSKLT
                     VSSSNTEEKNIDIEINVRKDILEDKGDGDLWRHRRLRWLNSTIGIDSLPVNPYENMKL
                     HDRQITATKKDVTVAENGAIESIQVNDQMIYSHPFEFVIETSKGKFKLNSKNIVIEKD
                     AEGLVSWKSSDNMEQGIKLDVSAYMEYDGYIRYNVKLSSEGEKQIKNVSLLNHFSPES
                     SEYFIGAGFKGGLRPQNYTWKWNGPYDSFWIGGEKSGLHVELRGGTYHGPLINDYKPA
                     PPKTWYNEGQGKVRVYENNAKDDIVVEVSTGELKVEKSIDFEFAFLITPVKPIDLKKH
                     FSQKYYHSSSEGFEDAAKEGANIINIHHSRSLNPVINYPFIVRDSLISYIEEQHKNNR
                     KVKLYYTIRELTNYTEEIFALKSLNHEIFVSGVGYGLPWQCEHLIDDYKPAWYTQLPD
                     QMSDAALVLNGFSRWINYYLEGLRWMLENYEIDGLYMDDVSFDRSVMKRIRKILEEYR
                     PGSLIDLHSNTGYSIGPANQYTDFFPYVDRLWFGESFKYNEMMPDEWFVTFSGIPFGV
                     MSEMLQDGGNRFLGMVYGTTGRHSYSQYSPAPIWKLWEDFKIDESKMIGYWDEKSPIK
                     TNHENVKATLFIKPDQILISVGNFYSEEKNVHLNINWRELNIDPEKAILEIPEIENFQ
                     SQDILSVNDKFIIKNKEGIIFILKEK"
     gene            complement(7143..>9529)
                     /locus_tag="PLI30_03850"
     CDS             complement(7143..>9529)
                     /locus_tag="PLI30_03850"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOI78721.1"
                     /translation="PSGQATEAVLTENAGPYAELMVGTYSDNQPDYSWIKPYEVKQWE
                     QYWYPIKGIEGFKNANLNGAVNLEKRNDKKGIKVFLGYYSTGRVDNAKVILKNKDKII
                     FEKNIAISPEKPFAESIKIEGTYELSDLYTQLINNNTGEILVDYQHIEREPIANEDIP
                     KPWRGYPAVDKLETVEELYLTGKRVEQFYAPRENPMNWYNAALAKDPGDSRTNIAVGN
                     IYLKNGDFTTAREYFAKAIKRLTNDYTVPSTHEAFYLQGLALRGLGLLAEAEDTLYRA
                     TWDHAYHSAAYYQLAELSCSRNDFANALHQINESLSTNSRNNKAITLKASLQRKNRDY
                     DGALTTLAAISGDDPLDFRIRNEYYLIAKESGDNQKANSLLASLNKEMRDFDDNYLEL
                     AVGYINNGLLSEAEDVLKRFNGENPFFAYYLGYLAYINGNSDQAQQFFKSAAGKSVDY
                     VFPYRLESVNVLNTAIKYNPTDGKAYYYLGNILYEKQPDAAIMHWKNAIKLNPELAIA
                     YRNLGWGYFYHLNNVPEAITHYEKAIALNRNEALYFTELSELYETNNTPIETRIKLFE
                     GNTEIAKNRDDSYMYYIENLILSKQAKRAVEALEGANFAYTEGSSRSRNIRINANLML
                     GKQYYDNKEYQKALDTFLKAKITKQEAGDDRLGGREVEVDYYIGLAYEALGKRSKANE
                     SFRSSANQPSQTVNVTSYYQGLSFAKLGKNDQAKKIFESLVSEADQQMKQSMDSEVGA
                     IFGRREAADNRLSRLYTMRGLGYKGLGNLEKAKDDLNKALELSQSNLTAIIEEL"
BASE COUNT         2543 a         2063 c         1812 g         3111 t
ORIGIN      
        1 agcagggatg ccccgtcgtc gtccaccagg cgtccctcca cgtacgtttg cagatcggca
       61 cacacctcca gcactttcag accggtaacg tattctgcca ggtgccaggc atgcgttcct
      121 atgtcgccca tgcaaccggc cttgcccgtg gtctttggat tggttctcca tccggcatta
      181 ttccctccct gcagctctat gcgttgcgac agccatccct gggtatactc cacgtatact
      241 ttgcgcagtt tacccagttc tccgtttgcg attcttgcct ttgcctcctt gactgccgga
      301 tatcccgagt atacgtgggt cagcgccaga accagtcccg tctcttccac cttctccttc
      361 agcttgaggg cctcttccag tgaaaaagtc atcggtttgt cgataaccac gtgaaaccct
      421 ctttgcaatg ccatcatagc gggttcaaaa tgccacttgt tcggtgtgac tatggttaca
      481 aaatccatcc gttctccggc aggcagctcc atctccttct cgaacatctc caggtaggag
      541 tcgtaaatcc ggtgatccgg gaggaaatat tccctgcctg agcttctgga gatttcagga
      601 ttgacgctga agcagccaca aaccagctca atgtagttgt ccataaaggc agcacgacgg
      661 tgtatggccc cgataaaggc atcgctgccg ccgccgatca tgcccatttt cagttttctt
      721 tgtttcatgt ttatgtaatg tttatttaat tgagattcaa tttacaatca aacggaaaat
      781 catgcccgct cccgccgagt ccctatgtcg tcccgggtat tgtttccttt atattatgcc
      841 tcttctttac atcttccctg aacaggaaca ggaacccggc aaggaaaacg attgccatca
      901 ggaaaggaac cgtccatatg gatatccagt cgtatcttcc ctgttcggaa tagaggtcca
      961 ccacgtttcc ggcaatcaac gtgccgaggt atttgcccac cccgtacgtg gcaaaagcga
     1021 tgagaccctg cgcggagtta cgcagcgagg agggagcctt ttcatccacg aacagctgcc
     1081 cggcaacgaa aaagaaggta aagcagaacc cgtgaagcgg caacaccgcg tagatgaatc
     1141 cgttctggcc ggtgctggct cccagcgtga aaagcccgta catcagaatc catgccgcga
     1201 tggcggcgac gatactgccc ttgtaccgta acttgaggaa gaagatgggg aagatgaaca
     1261 ggaagatcac ctcggcaacc tgtccaaggc tcatggctgc ggccgcattc tcgatcccca
     1321 cgtccgtgat gaagaggttc gtgaaggagt catagaatgc cagcgggatg cagatgagcg
     1381 ccgagaagac gagtaggacc aggaaagacc tgtccctggt cagtttgaac gcgtcgaacc
     1441 cgagcagttg acgcacgtcc gctttctcca tagtccgcac gggagcgtta tggggcaggg
     1501 tcagcgcgta tactccggcc agcagggaga tgcctgcccc gatcaggaat ggggtggagt
     1561 agggctcaat cttcagggcg cttatcagta gtcctgaaca gatccagcca aacgacccga
     1621 aaagccttat cttggaaaac tccctggccg gatcgcgcag gttagcaaac gatatggaat
     1681 tggtcagcga catggtcggc ataaaacaga tactgtagag caacaatacg gggaagaaca
     1741 aggagaacga ttcgatgcgg gtcagggaaa atagcaggat ggctcccacg atgttcagga
     1801 atgccagtac cctttttgcc ggaaaaaacc ggtcggcgac cattcccacg atcaccggtg
     1861 atatgatggc tgctatggca gcggtgctgt atgccaggcc gatttgaact ccggaaaagt
     1921 cgagggtcct tcccaggtag gttcccatgg tcacgtacca tgagccttga ataaagaact
     1981 ggaagaacat catggcggat agttgaatct tcttgttttt gttcatttgg attgtttaag
     2041 aatttaataa ttcacgcgat tacgaaaata aattatttat aagattcata atctgttgga
     2101 tttacccaat ttgtttgttt ccacggttcc attatttttc ctgaaactac tcgttcccaa
     2161 aagtcccatg cattttctga ggaaaaggga taactatcaa acacgttccc attattaaac
     2221 atacgtggat cattttgtgc acgaagctgt tcaaagagtt tattcttcaa cctctctttt
     2281 tgtttagaat aggtggggtt gtcgatcaaa ttgatgatac aatccttatc cagagagagg
     2341 ttatataatt cttccggctc tcttttaccg aatgactgtt ggaaatacct gttattttgt
     2401 ccgcttcgtt tcatatttaa aattgtagtt ttcgttggag aaccatcaca gtcgagatat
     2461 ccggtttccg attctccagc tggcaacaaa tctggtttca ggtttaatat atacaataga
     2521 ttgtcctgaa taatccctct ggttgggtat ccctggttcc cacgacgtcc atagtcatga
     2581 cgttctctcc cgagtagagt gaaattccgg ctatgagtaa caatgcctgc ttttttattt
     2641 ttgaatattt cagtcaagct ttttccggtt ggaattatca ttccatgctt tatcggattg
     2701 gttttgctaa cttccaaaaa ggtgggagta aaatccacaa aattgatata atcctctacc
     2761 actctgccgg gattgataat tccatttttc cacattatag tcaaaggcat gtgattggat
     2821 atctcataac tgttggcttt actccttggg aaaggcatcc cgttgtctga tgtaaatatt
     2881 attagggtgt tatccaacaa gtctctcctc tccaattccg aaattattgc agccagatgt
     2941 ttatccaaat attcaatctc gaaagcataa tccagcatat ctgtacgaac gacatcatta
     3001 tcgggccaat acatcggtac cttatcaatc atttcttttg attttcctcc aaatttctca
     3061 ccagaaccat attcatattc cctgtgaggt tcagtgaatc ccatccaaaa gaaccacggt
     3121 ttgtcttcac ttgctctatt aagaaaatct ttgaagttct ctgtataatc gttctttcct
     3181 atatatgggg tagggggaat caaagtatat ttttgaaacg gggtgccggt caattcacgc
     3241 tgctcacctt cttttgtatg ggctgttccc ggggcccatc ctttaccaga gaaagctatc
     3301 tggtagccgt tttcttttaa tacttccgtc actactttta tatccgatgg aaactgaggt
     3361 atatggtttc cggcttcttt tagttgccaa gagtataaac cggtcaacac acatgctcta
     3421 gatggcgccg atttagcatt aggtgtatag cagtttgaaa ataaaattcc ttcacgggcc
     3481 atcctatcaa atcctggagt gtttacccat ttactcccat tagccccgaa atgcggatat
     3541 gatgcatcat cagcaataca aaagagaata tttggccgct gagagggcct tttttgagct
     3601 aaaagactaa aagttaaggt ttctgtcacc agaaaaagag aaaacagtaa tgatttttgc
     3661 atttgcaggt ttattaaata attccctttc aagcacatct tttcaattgt gacatgaccg
     3721 gattaaacat tagatctctc acttttgata ccttcgtcta taaagagaat aaggcagagg
     3781 aaaaaacttt caggagccaa gctagggttc ttacaaaaaa gatttacggt gtaaagcgct
     3841 cttttttgta attattcaac caatccccag tctaatgcgt taatgggtat aaaatgatgt
     3901 gtacagctaa tctgactgga ttaagcgatg gcacaaatat cgagttattt ttcttttaag
     3961 ataaaaatta ttccttcttt atttttaata ataaacttat cgtttaccga taggatatct
     4021 tgtgactgaa aattttcgat ttccgggatt tcaagaattg ctttttcagg gtcaatattc
     4081 aactctcgcc aattaatatt taaatgaaca tttttttctt cactgtaaaa atttccgaca
     4141 gaaattaaaa tttgatctgg tttgataaac agagttgctt tcacattttc atgatttgtt
     4201 ttaattgggc ttttttcatc ccagtatcca atcatttttg attcatcaat tttgaaatct
     4261 tcccaaagtt tccaaattgg agcgggagag tattgggaat aggagtgcct gcctgttgtt
     4321 ccataaacca tacccaagaa tctgtttccc ccatcttgta acatctcact cattactcca
     4381 aaaggaattc ctgagaaagt tacgaaccat tcatcgggca tcatctcgtt gtatttaaag
     4441 ctttctccaa accatagacg atcaacataa gggaaaaaat ctgtgtattg atttgccgga
     4501 ccaatcgagt agcctgtgtt agaatgtaaa tcgattaaag aaccaggacg atactcttct
     4561 aaaatttttc gaatgcgttt cattacagaa cggtcaaaag aaacatcatc catatataat
     4621 ccatcaattt cataattttc aagcatccat cgcaatcctt cgagataata gtttatccag
     4681 cgtgagaacc cgtttagtac taatgctgca tctgacattt gatcaggaag ttgagtatac
     4741 cacgccggtt tataatcatc aatgagatgt tcacactgcc aaggcaatcc ataacccacg
     4801 ccggaaacaa aaatctcgtg attcaggctt tttaacgcaa atatctcttc cgtataattg
     4861 gttaattccc gaatggtgta atatagttta accttacggt tgtttttatg ttgttcttca
     4921 atataactta ttaatgagtc ccttactata aaaggataat tgatcacagg attcagactt
     4981 ctggaatgat gaatatttat tatgttagca ccttcctttg ctgcgtcttc aaatccttct
     5041 gagctggaat gatagtattt ttgtgaaaaa tgttttttta aatcgattgg ttttacaggg
     5101 gtaattaaaa aagcaaattc aaaatcaatt gatttttcaa cttttagttc gcccgtggag
     5161 acttcaacaa ctatgtcatc tttggcattg ttctcatata ctcttacttt gccttgccct
     5221 tcattgtacc atgttttcgg cggcgctggt ttataatcat ttataagcgg gccatgataa
     5281 gttccacctc tgagttctac atgcaaacct gatttttctc caccaatcca gaaactatca
     5341 tagggaccgt tccacttcca tgtgtaattt tgtggcctga gtccaccttt aaatcctgct
     5401 ccgataaaat attcagatga ttcaggtgaa aaatgattca gcaaacttac attttttatc
     5461 tgtttttcac cctctgatga tagtttgaca ttataacgta tgtacccatc atattccata
     5521 taagcagata catccagctt aattccctgt tccatgttgt cagatgattt ccaactaact
     5581 agtccttccg catctttttc aataacaatg tttttactgt tgagtttgaa tttacctttt
     5641 gatgtttcga taacaaattc aaatgggtga ctataaatca tttgatcatt tacttgaatt
     5701 gattctatgg caccattttc tgcaacagtc acatctttct tcgttgcagt tatctgacga
     5761 tcatgtaatt tcatgttttc gtaaggattt acaggtaagc tgtcaattcc aatggttgaa
     5821 ttcaaccatc ttaatcgtct atgccgccat agatcaccat ctcctttatc ttcaagaatg
     5881 tcttttctga catttatttc aatatctatg tttttctctt cagtgtttga tgatgaaaca
     5941 gtcaatttac ttttatatct gccaaccttt gcatcgtttg gaatctgtac tccaaaccat
     6001 aatgcttgta ctttatttgc tggaacatga acatcaaatt ttagtggaga accgtcccag
     6061 tttactcctt ctaaattaaa gcaggtaatt tgctctttag ggatattgta cttatttgat
     6121 tcatggataa gatcggtaaa agtaaccttg acatcacgca attctttctc tattgcccat
     6181 aggcctaact gccaggcata atattcattt cttaaagcat cgccttcaaa tcggagggag
     6241 gggccatttt ttacccaccg ataggggatg gtagtgagtt ttataggaaa tgcacgatct
     6301 tcaggaaaag taagaaagtt ggatttattt ccattcaata atttatattt ttcattttca
     6361 gtagcgatta ctcccattga agttaaaaag tcaaacttag ttctgctttc aaatcgtgtt
     6421 actgttgcat tcgggatatt ttttaggttt ttaagaacgt tttcctccca atttttatcc
     6481 gtctcatatt caggagtagt gtaatcgttc cacggttttc cataacgtgc atcccccgaa
     6541 ccttttctgt atttatacgg cagataataa acgatgtaat cactttcatt ttctacgggc
     6601 ttgaaagcga tggttccttt ttctgctgtt tgttcaatca cactaacgtc ttttatttca
     6661 tttccattac ttgcgtctgt gacaattatt ttttttgttt ccggacggag gtctgatctt
     6721 ctccatggga ggttaacgat gactgcttca tgttcctgtt tatcaacatg aacaatagct
     6781 ctgtgatttc ctcttccatc aacatcccat attgtatcag ctattacaaa tggaattccg
     6841 tctggtgcaa actgttgata tttgtcctct ccattgttat attgcgtgga agagcagctc
     6901 agcaaaagag ctgaaaaagc acaaataatt ataaatttca tacgattaaa attatttatt
     6961 ttcactaaag ttagatattt gatatgccat ggcatagacc gaacttgttc ggtctatgct
     7021 tagcctgctt aacacaaatg ttcaaatgtg tcccattaaa aatgagacgt tgttaaagat
     7081 caactaattt tggttcattt gtgttattaa tggaataata atgaatactt gtatttttta
     7141 ttttataatt cctcaataat tgctgtcagg ttactctgag ataattccaa tgccttattc
     7201 aaatcatcct tagccttttc taagtttccc aaacctttgt atcccaatcc acgcatggtg
     7261 tacaatcgtg acaatctgtt gtctgcagct tcacgtctgc caaagattgc accaacttcc
     7321 gaatccatag actgcttcat ctgttggtca gcttccgata cgagggattc aaatattttc
     7381 tttgcttgat cgttttttcc aagcttagca aaactaagtc cctgataata tgacgttaca
     7441 ttgacagttt gcgagggttg attggctgaa cttctgaatg attcgtttgc ttttgagcgt
     7501 ttacctagtg cttcgtaagc taaaccaata taataatcca cctctacttc tcttcctcct
     7561 agacggtcgt cacctgcttc ttgcttggtt attttagcct ttaggaaagt gtcaagcgct
     7621 ttttgatact ctttattatc ataatattgt ttgcctaaca ttaagttagc attgatcctt
     7681 atatttctgg atctggatga accttcagtg tatgcaaaat ttgctccttc cagggcttca
     7741 acagccctct tagcctgttt tgaaagaata aggttttcga tataatacat ataggagtca
     7801 tcccgattct ttgcaatctc agtgtttcct tcgaagagtt ttatccgcgt ttcgatcgga
     7861 gtattgtttg tctcataaag ttcacttaac tcagtaaaat acaatgcctc atttctattt
     7921 agggctattg ctttttcata atgagttatt gcctcaggta cattgtttaa atgataaaaa
     7981 taaccccatc cgagattcct gtatgctatt gcaagttcag gattgagctt tatggcattt
     8041 ttccagtgca ttattgcagc atcaggttgt ttttcataca atatatttcc caaataataa
     8101 taagcctttc catccgttgg attatacttt atggcagtat tcaatacatt aaccgattct
     8161 aggcggtaag ggaatacgta atctacggac ttccctgcag cagatttaaa aaattgttga
     8221 gcctggtcgc tgtttccgtt tatataagct aaatagccaa gatagtaggc aaagaacggg
     8281 ttttcaccat tgaaacgttt tagtacatct tctgcttccg acagcaatcc attgttaata
     8341 tatccgacag caagttcaag ataattatca tcaaaatctc tcatttcctt attaagagag
     8401 gccagcaggc tatttgcctt ctgattgtcg ccggattctt tggcaataag gtaatattca
     8461 tttctgattc tgaaatcaag tggatcatct cctgaaatgg cagctaaggt agttaatgcc
     8521 ccgtcataat ctcggttttt ccgttgtaac gatgctttta acgttatagc tttgttattt
     8581 ctactatttg tagacagtga ttcgttgatt tgatgaagcg catttgcaaa gtcattcctg
     8641 ctgcacgaaa gttcagctag ttgatagtag gctgccgaat gataggcatg gtcccatgtt
     8701 gcgcgataga gagtatcttc agcctcagcg aggagtccta aacctcttag tgccaatccc
     8761 tgcaaataga atgcttcatg tgtagaggga accgtgtaat cattggtcaa tctcttaatt
     8821 gccttggcaa aatattccct tgcagtggta aaatcgccat ttttcaaata gatattaccc
     8881 acggcgatat tggttcgact atcacccgga tcttttgcta atgctgcatt gtaccaattc
     8941 attggatttt cccttggagc ataaaactgc tccaccctct ttcctgtcag atacaactcc
     9001 tctaccgttt caagtttatc gacagcggga tagccacgcc atggtttcgg aatgtcttca
     9061 ttggcaattg gttctcgctc gatatgctgg tagtcaacaa gtatctcacc ggtattgttg
     9121 ttgattaact gtgtatacaa gtcactcaat tcatatgtac cttcaatttt tatgctctcg
     9181 gcaaatggtt tctctggaga aattgcaatg tttttttcaa atataatctt gtccttgttc
     9241 tttagaatca cctttgcatt gtcgactctc cccgttgaat agtagccaag gaagacctta
     9301 ataccttttt tgtcatttct tttttctaaa ttaactgctc cgttaagatt ggcatttttg
     9361 aaaccctcaa tgccttttat aggataccag tactgttccc attgcttcac ttcgtaaggc
     9421 ttgatccagc tgtaatcggg ctgattatcg ctatacgtac ctaccattaa ttctgcataa
     9481 gggccggcat tttctgttaa cactgcttca gtagcttgtc cgctagggc
//
LOCUS       DAOSDR010000063         9451 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrimonas sp. isolate AMR_MDS_1826
            NODE_2706_length_9451_cov_3.713495, whole genome shotgun sequence.
ACCESSION   DAOSDR010000063 DAOSDR010000000
VERSION     DAOSDR010000063.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863355
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrimonas sp. (wastewater metagenome)
  ORGANISM  Petrimonas sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Dysgonomonadaceae; Petrimonas.
REFERENCE   1  (bases 1 to 9451)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9451)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:10:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,720
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 35
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9451
                     /organism="Petrimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2706_length_9451_cov_3.713495"
                     /isolate="AMR_MDS_1826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2023866"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..847)
                     /locus_tag="PLI30_03855"
     CDS             complement(<1..847)
                     /locus_tag="PLI30_03855"
                     /inference="COORDINATES: protein motif:HMM:NF026257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HOI78722.1"
                     /translation="MNEPAVSLFLELLRSAIWNRAADEDLFRHVGSTVWEEVISLAES
                     QRVSALLYDGIMTLPTALRPGKKEVYTLFLQAEAIEKLNKGLNDELGNLAMEYGKIGC
                     PFVLLKGQANAILYPRPEHRAPGDIDLFLYRKGDYEKANEWAKKKGCRMDAENIHHQS
                     YEINGIHVENHKNICYFGIRKYDGLLEEKMQEIIRNHRFIELEIDSLKVSVLPVEFNA
                     FFLFYHLFHHFIHLGVGVRQFCDWVLFMHTHSPQMDKEALTGLARQFDLLNAMEVFAS
                     AAVRYL"
     gene            complement(840..1109)
                     /locus_tag="PLI30_03860"
     CDS             complement(840..1109)
                     /locus_tag="PLI30_03860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="PqqD family protein"
                     /protein_id="HOI78723.1"
                     /translation="MKIKENIRIKQIGDESILVSHDGENMNYTRVISLNKSAEFLIAG
                     SFGADFTVQGWAERLTGRYGIDKTQAVADAQVLADKLMKAGAIYE"
     gene            complement(1157..1996)
                     /locus_tag="PLI30_03865"
     CDS             complement(1157..1996)
                     /locus_tag="PLI30_03865"
                     /inference="COORDINATES: protein motif:HMM:NF039669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6146 family protein"
                     /protein_id="HOI78724.1"
                     /translation="MESLIVPNEVFFSHILEEIEARRAVRIPVKGNSMLPFIRPLTDQ
                     VELSELTGQSVTKGNIVLARTNGSNYVVHRIEKVEGEVVTLRGDGNLSVRERCDKGNV
                     VAEVTAVYKKGRKISKGSLEWNLARDCWFTSPLLRRFYLGISRNMKKIFFLFTVLAFL
                     AACSGPKGMVKIEPPQEDSVEYELIVFDPGFETWYTLQNSPARYRSQEYYEGWNQQYV
                     SEWNYLATQPRRRSFFESIIGYEPGVDYGFELNHKLFYYFQYVEHVLRIPILPNGPSG
                     VIY"
     gene            complement(1999..2871)
                     /locus_tag="PLI30_03870"
     CDS             complement(1999..2871)
                     /locus_tag="PLI30_03870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI78725.1"
                     /translation="MNEKLKYSVAGHLFCIETPDRARTTGIMPNYTPFRVENSSGDDF
                     LFSLRGHREVHLPEFPPDDTMEWNGVDYRVYHSPEGMVVSMKQGKKEHRFFAPADWKE
                     VVCDLSFTDKNEAVFLNSFLRLAFGVTSILANRTIKIHASVTELNGKALIFLGKSGTG
                     KSTHSRLWREFVPGCTLLNDDEPLIRVFEDEPVRVYGAPWSGSTACFRNASAEVAAFI
                     HLYQSPENRLTRLRNVEALSSLYASAAMLRSDAGNKDRVLDVVAAVLQRVPVYRLDCR
                     PDYEAVSLTRSLLP"
     regulatory      2925..3190
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            3297..5318
                     /locus_tag="PLI30_03875"
     CDS             3297..5318
                     /locus_tag="PLI30_03875"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HOI78726.1"
                     /translation="MNVLKRNAVIVAALYAVTTPLLAQTSLDSLQHLPEVVVTERYGD
                     REIRSSAPMRILLRKSIRNLNALQLSDVVKHFPGVTVKDFGGIGGLKAVSVRSLGASH
                     TAVNYNGFTINDIQTGQIDIGRFALDNVDMISLNSGQSDNIFQPARLFASASVLNIQS
                     AAPQVGPGKKVNGRASLKAGSFGMFNPAVSTNLKLNEKLSASLSGEWLSANGEYPYIL
                     HYGEAGRDSSSVEKRENTDVKNLRLEGALFANFSDKSSGNLRTYYYQSERGLPGATIL
                     YNTSGFSKQRLRDNTFFTQAHYFNNLSGRWALQANAKYNHGYVYYLDPEFLNAEGKLE
                     NRYRQNEFYGSVSALFRALENLSFSASTDVVMNNMSANLPDFAYPTRYTSLSSLAAKY
                     VSDRLLATASLLYTQTSEHVRSGTAADNRHRLSPYSSLTFQPFEAIDFRLRAFYKNIF
                     RLPTFNDLYYSRVGKRDLKPEDTHQFNIGFTYSTSAGERLPLLTMTTDAYFNKINNKI
                     VAYPNRNIFEWSMMNFGSVEICGVDIALESAFVLSSKVKLLAGSSFTFQDSRNKSNSG
                     SPAYNHQLPYTPRFSGSGRAVVETPWLNAAYTLLWSGTRYTSAQNMPENRLSGYADHG
                     ISLSKSVEIKHNRIGLSVEALNLGNKNYEIVRNFPMPGRSFRGTISINF"
     gene            5331..6407
                     /locus_tag="PLI30_03880"
     CDS             5331..6407
                     /locus_tag="PLI30_03880"
                     /inference="COORDINATES: protein motif:HMM:TIGR02276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YncE family protein"
                     /protein_id="HOI78727.1"
                     /translation="MKKYFVFILLLTTAFFTACTPETVIDETELPAKEAKRILILSEG
                     FYGDNSASLAHYDFDKQVLTKDYFTSVNQRGLGDTANDAIRYGAKIYVVVNSSSTVEV
                     IAANTGKSLKQIPMKTATNQAKQPRYAAAYGGKVYVTSHDDTVTRIDTTTLSIDGSVQ
                     VGADPEGICIANSNIYVANSGGMNFPNYDKTVSVIDLKSFTETKKIEVGLNPKRVYAD
                     KEGDVYVYSVGNYTTVPATFQKITPKGEVAAFDEVKNIMDFTLSGDKAFIYYKDYATS
                     ETVVSVFDCLNDKLLTGKLIADKSIAIPYSITVDPFNGDIYLTDAVEYVKILGNVYCY
                     DKTGKRKFTLENPGYLPNRVLFLN"
     gene            complement(6411..6812)
                     /locus_tag="PLI30_03885"
     CDS             complement(6411..6812)
                     /locus_tag="PLI30_03885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI78728.1"
                     /translation="MKSKLFLLTLFLIGSVSFVEAQYVNEVAISDIDADYVMIWSDSG
                     PILTSKITVRVDYGQGGRTPQITDERRRPMSFNGMLAVLNLMSRNGFELFEIMRSEKD
                     NREFFLLRRKNSFSGNGSRDAVDYDTNVRSR"
     gene            7012..7836
                     /locus_tag="PLI30_03890"
     CDS             7012..7836
                     /locus_tag="PLI30_03890"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HOI78729.1"
                     /translation="MRSHKTSILIINTEGKRNKTLLVPTRILLHWKKYAAVAVLVVLC
                     LIAALGVTVFHKTSKIYQHEIAKADKIKSLVDVPKAQQTFQSIDESIFRINELLKSRG
                     LVQMEVENLGGPENVEVVKINELADFYREQLDNLEKTLKNVPIGNPTPSDKIISSYGY
                     RRNPFTGRGAEYHSGIDIKGERGTPVKTTANGKVEVAGWYGGYGKCVIVKHENKLKTL
                     YAHLSEITVREGDEIESGQVIGRLGSTGRSSGPHLHYEIIKDDEKVNPGDYINFHE"
     gene            7844..8251
                     /locus_tag="PLI30_03895"
     CDS             7844..8251
                     /locus_tag="PLI30_03895"
                     /inference="COORDINATES: protein motif:HMM:NF016408.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HOI78730.1"
                     /translation="MRNSKSKSKSKSDHSIESQPIDTVIGTDILFKGDIRGESIIRVD
                     GTVEGNLSLSKGIVVGEKAKVTGNLKSDRIIIYGSISGDIECKELNIKSSGTVNGNIQ
                     ADNVEIEMGGKYNGNLSMQPSPPPSSAKDKEDR"
     gene            complement(8261..>9451)
                     /gene="lepA"
                     /locus_tag="PLI30_03900"
     CDS             complement(8261..>9451)
                     /gene="lepA"
                     /locus_tag="PLI30_03900"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004325757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HOI78731.1"
                     /translation="NPFRGIIAYFKIMNGSIAKGDLVKFVNTGKEYDADEVGILRLGM
                     DPRQRVECGNVGYIISGIKTSKEVKVGDTITHVKNPCDKAIAGFEEVKPMVFAGVYPI
                     ESEEFENLRASLEKLQLNDASLTFQPESSVALGFGFRCGFLGLLHMEIIQERLEREFD
                     MDVITTVPNVSYKVYDKKGNMKEVHNPAGLPDPTLIDHIDEPYIRSSIITSTTYIGPI
                     MTLCLGKRGILIKQEYISGDRVEIIFDLPLGEIVIDFYDKLKSISKGYASFDYHIHDF
                     RPSKLVKLDILLNGEPVDALSTLTHADNAQSFGRRMCEKLKELIPRQQFDIAIQAAIG
                     AKIIARETVKAVRKDVTAKCYGGDISRKRKLLEKQKEGKKRMKQVGNVEVPQKAFLAV
                     LKLD"
BASE COUNT         2428 a         2554 c         2255 g         2214 t
ORIGIN      
        1 ccaggtacct gacggctgcc gaagcgaata cctccatggc gtttaacaaa tcgaactgcc
       61 tcgccaggcc tgtaagcgcc tctttatcca tttgcggtga gtgggtatgc atgaacagta
      121 cccaatcgca aaactgacgg acaccgacac ccaggtggat aaaatgatga aagaggtgat
      181 aaaagagaaa aaaagcattg aactcaacgg gcaagacact cactttcaag ctatcgattt
      241 ccagctcgat aaagcgatgg ttccgtataa tttcctgcat cttttcttcc agcagtccgt
      301 cgtatttcct gatcccgaaa tagcagatgt ttttgtggtt ttcaacatga ataccgttga
      361 tttcatagga ttgatgatgg atattttccg catccatccg gcatcctttt tttttggccc
      421 attcgttcgc tttttcgtaa tctcccttcc gataaagaaa caggtcaata tcgccgggag
      481 cacgatgctc cggcctggga taaaggatag cgttggcctg tccttttaac agcacaaagg
      541 ggcaacctat cttcccgtat tccatcgcaa ggttccccag ttcgtcgttc agccctttgt
      601 tcagtttctc aatagcttca gcctgcaaaa aaagcgtgta cacctccttt tttcccggcc
      661 gaagggctgt aggcaacgtc atgattccgt cgtacaacaa ggcactcacc cgttgagatt
      721 ccgccaggct gatcacctct tcccaaaccg tagagccaac atgccggaac aggtcttcgt
      781 cggccgccct gttccagatg gcggagcgga gcaactccag gaaaagagaa acagccggtt
      841 cattcataga tcgcaccggc tttcatcagt ttatcggcca gtacctgagc atcagccacg
      901 gcctgtgttt tgtcgatacc gtatctcccc gtcagccttt ccgcccagcc ctgaacggta
      961 aagtccgcac cgaagctccc ggctatcaga aattccgctg atttgttgag cgatattacg
     1021 cgggtgtagt tcatgttctc cccgtcgtgg gaaaccagga tggactcatc ccctatctgt
     1081 ttgattctga tgttctcttt tatcttcatt tgacttgtta tttttattaa agccgtttac
     1141 ggcgtacgcc cttcaatcag tatatcactc ccgaagggcc gttgggcaat atcggtatcc
     1201 gcagcacatg ttccacgtac tggaaatagt agaacagttt gtggttcagt tcaaacccat
     1261 aatccacacc cggctcatac cctatgatag actcaaagaa cgatcttctc cggggctggg
     1321 tcgccaggta attccattcc gacacatatt gctggttcca accttcgtag tactcctgag
     1381 aacgatacct ggccggggaa ttctggagtg tgtaccatgt ttcaaatccc ggatcaaata
     1441 caatcagctc gtattccacc gaatcttcct gcggcggttc aattttcacc atcccttttg
     1501 gccctgaaca ggctgccagg aatgcgagaa cggtgaataa aaaaaatatt tttttcatat
     1561 ttctgctaat tcccagataa aaccttcgta acaacgggct cgtaaaccaa caatctcttg
     1621 ccaggttcca ttcaaggctt cccttgctga ttttccttcc cttcttataa acagccgtca
     1681 cctccgcaac gacatttccc ttgtcgcatc gctcgcggac agacagatta ccgtcgcccc
     1741 tgagcgtcac cacctcccct tcaaccttct cgatcctgtg aacaacatag ttgctcccgt
     1801 tcgtcctggc cagcacgata ttcccttttg tcaccgattg cccggttaat tccgagagtt
     1861 cgacctgatc cgtcaacggg cggataaaag gcagcatact gtttcccttt accggaatcc
     1921 tcaccgcacg gcgcgcttca atttcctcca gaatatgcga aaagaagacc tcgttcggga
     1981 caatcaggga ttccattctt acggcaataa cgagcgggtt aatgacacag cttcatagtc
     2041 cggccggcaa tcgagccggt aaaccggtac acgttgcaaa acagccgcaa caacatccag
     2101 cacccggtct ttattaccgg catccgaccg gagcatcgcc gccgaagcat acaacgaaga
     2161 gagcgcttca acgttcctga gccgtgtcaa ccgattttcg gggctctggt acaagtggat
     2221 gaaagcggca acctccgccg acgcgttcct gaagcaggcc gtgcttccgc tccagggtgc
     2281 cccgtaaacc cttaccggct cgtcttcgaa cacgcgtatc agcggttcat catcgttcag
     2341 cagcgtacag ccaggcacaa actctctcca cagccggcta tgggtgcttt ttcccgttcc
     2401 gctcttacct aaaaaaatca gcgccttgcc gttcagttcc gtcacggagg catggatctt
     2461 gatggtccgg ttcgcaagga tggaggtcac gccaaaggcc agcctcagaa aactattgag
     2521 aaagacggct tcgtttttat cggtaaacga gagatcgcaa accacctcct tccagtcggc
     2581 cggggcaaag aaccggtgtt ctttcttccc ttgtttcatg gagacaacca tcccttccgg
     2641 tgagtggtac acccggtaat ccacgccgtt ccactccatc gtatcgtccg gcggaaattc
     2701 gggcagatga acctcccggt ggcctcttaa actgaacaaa aaatcatcgc cggaactatt
     2761 ttccacccgg aaaggggtgt aattcggcat aattccggta gtacgtgccc tgtcgggggt
     2821 ttctatgcag aatagatgcc cggccacact gtatttaagt ttctcattca taactcaaca
     2881 caaagatata tttttttatt ttctttccac ttttttttac ctatctttgt cctcgaaatg
     2941 ttggtgttac gcgttccaga tttggcttcg gaaccgtaat gaaaagggaa tcaggtgaaa
     3001 atcctgaaca gacccgctgc tgtaagcttc gtttcacgtg ctgaacagat caccttttgc
     3061 cactaaaacc cgctcctgct tccccacaag ggaggcacta gcttcggaaa gaagctcgaa
     3121 aaccggtttt gggaaggcgt tcaggacacg gaagtaagtc agaagacctg ccatcgtttc
     3181 gcacctgtta atggctttcg ggggatagag cttgaaaacg actcgcgaac agaatctttt
     3241 ttatttacgc gcttcatttt cagaatgctt taccggaatt aaaaattcgg tataagatga
     3301 atgttttaaa aagaaatgca gtaatcgttg ccgcacttta tgcggtaact actccgttgc
     3361 tggcacagac cagcctcgac agcttgcagc acctgcccga agtggtcgtg acggagaggt
     3421 atggcgaccg ggaaatccgc tcatcggctc cgatgcgtat tttattgcga aaatcgatcc
     3481 ggaacctcaa tgcgttgcag ctgtccgacg tggttaaaca ctttcccggc gttaccgtga
     3541 aggacttcgg gggtatcggc gggctcaagg ccgtatcggt acgcagcctc ggtgccagcc
     3601 acacggcggt gaactacaac ggctttacca taaacgacat acaaacggga caaatagata
     3661 tcggccggtt tgccctggac aacgtcgata tgatctccct gaactcggga caaagcgata
     3721 acatctttca accggcccgg ctgttcgcgt cagcctcggt actgaacatc cagagcgccg
     3781 caccacaggt aggcccggga aaaaaggtca acgggcgggc ttcgctcaaa gccggatcgt
     3841 tcggcatgtt caacccggca gtctccacca acctgaaact taacgaaaag ctatcggcat
     3901 cgttgagcgg cgaatggctg tccgctaacg gtgagtaccc gtacatcttg cattacggtg
     3961 aggccggaag agacagctca tcggtagaga aacgcgaaaa taccgatgtg aagaacctcc
     4021 ggctggaggg tgccctgttc gccaactttt ccgataaaag cagcggaaac ctgcgcacct
     4081 actactacca gtcggaacgc ggactgcccg gagccaccat cctctacaac acgtccggct
     4141 tttccaaaca acggctccgg gacaacacct ttttcacgca ggcacactac ttcaacaacc
     4201 tctccgggcg gtgggcctta caggcaaatg ccaagtataa ccacggctat gtgtattacc
     4261 tcgatccgga atttttaaat gcggaaggga aactggaaaa caggtaccgg caaaacgaat
     4321 tttacggatc ggtttctgcc ctgttcaggg cgttggagaa cctctcgttc tcggcatcaa
     4381 cggatgtcgt gatgaacaac atgtcggcca atctaccgga ttttgcctac cccacccgct
     4441 acacctcact ctcgagcctt gccgcgaagt acgtctccga ccggctcctg gccacggcca
     4501 gcctgcttta cacacagaca tcggagcacg tacggtcggg tacggctgca gacaaccggc
     4561 acaggctttc cccatactcc agcctaacgt tccagccttt cgaagcgatc gatttccgcc
     4621 tgagggcgtt ctacaaaaat attttccggc tacccacctt caacgacctg tattattcgc
     4681 gcgtggggaa acgggacctg aaacccgaag atacgcatca gttcaacata gggttcactt
     4741 attccacgtc cgcgggcgaa cggcttccgc tgctgacaat gaccacagat gcctatttca
     4801 acaagatcaa caacaagatt gtcgcctacc ccaaccggaa catcttcgag tggagcatga
     4861 tgaactttgg ctcggtggag atttgcggcg tggacattgc gctggaaagt gcttttgtgc
     4921 tttcctccaa ggtaaagctg cttgccggaa gttcgttcac ctttcaggat tcccgaaaca
     4981 agtccaactc cggcagccca gcctataacc atcaattgcc ctatacgccc cgcttctcgg
     5041 gttcgggccg ggctgttgtg gaaacgccct ggctaaacgc ggcttacacg ttgctgtggt
     5101 cgggaacacg gtacacctcg gcacagaaca tgccggagaa caggctgagc gggtatgccg
     5161 atcacggcat ttcgctgtcg aagagtgttg agataaaaca caacaggatc ggcctgtcgg
     5221 tcgaggccct caacctggga aacaagaact acgagatcgt ccgcaacttt ccgatgcccg
     5281 gacggtcatt caggggaacc atttccataa acttttaaat atttaaataa atgaagaagt
     5341 attttgtatt tattctgctg ctcacaactg cgttttttac ggcctgcacg cccgagaccg
     5401 ttatcgatga aactgaactt ccggcaaagg aagccaagcg tattctgatc ctttccgaag
     5461 gattttacgg agacaacagc gcgtcgcttg cgcattacga ctttgataaa caggtattga
     5521 ccaaggatta tttcacgtct gtcaaccaaa ggggtttggg cgacacagcc aacgacgcca
     5581 tccggtacgg agccaaaatc tacgttgtgg tcaactcgtc cagcaccgtt gaggtgattg
     5641 ccgctaacac ggggaaatca ctgaaacaga tcccgatgaa aaccgctact aaccaggcaa
     5701 agcaaccccg gtatgccgcg gcttacggag gaaaagtgta cgtcacttca cacgatgaca
     5761 ccgtcacccg catcgacacc acaacactga gcatagacgg ttcggtgcag gtgggagcag
     5821 atcccgaagg catctgtatc gccaacagca acatttacgt ggcaaactcg ggagggatga
     5881 atttccccaa ctacgacaaa accgttagcg taatcgacct gaagagcttt acggaaacga
     5941 aaaagattga ggtcggttta aatcccaaaa gggtctatgc cgataaggaa ggcgacgttt
     6001 atgtgtactc ggtaggcaac tatacaacgg tgccggcaac gtttcaaaag atcaccccaa
     6061 aaggcgaggt agccgctttt gatgaagtga agaacataat ggatttcacc ctgtccggcg
     6121 acaaggcatt tatctattac aaggattacg ccacctctga aaccgtggtt tctgtttttg
     6181 actgcctgaa cgacaagcta ctcaccggca agctcattgc cgacaaatcg atagctattc
     6241 cctactccat taccgtagac ccctttaacg gcgatattta cctgaccgac gccgttgaat
     6301 acgtgaagat attgggaaat gtctactgct acgacaaaac aggaaagcgt aagttcacgc
     6361 tcgagaaccc gggttacctg cctaacaggg tgctgtttct gaactaacgg ttaccggctc
     6421 cggacgtttg tgtcataatc cacagcgtcc cggctgccgt taccggaaaa cgaatttttc
     6481 ctccgcagca ggaagaattc ccggttgtct ttttcgctgc gcatgatctc aaatagttcg
     6541 aaaccgttgc ggctcatcag gttaagcaca gcaagcatcc cgttgaacga catcggacgg
     6601 cggcgttcat cagttatctg cggcgttctg ccgccctgcc catagtctac ccgcaccgtg
     6661 atcttcgatg tcaggatcgg gcccgaatcc gaccagatca tgacgtagtc cgcgtcaata
     6721 tcgcttatgg ctacttcatt cacgtactgt gcttcgacaa acgatacgct gccgataaga
     6781 aaaagtgtca gtaggaaaag ttttgatttc atgtccgtaa taattaaaat gatcagggcc
     6841 gggaatcaat ggattaagcc ggccgttcct ggaacaaaag taaacaaaaa aacaatacgt
     6901 taaaccggaa ctgctttgag gggatcccgc attttttatc ctaaaagtta acattctttt
     6961 aaaaaaaaaa ttatctttgc aatatcgttg tgctatttcc ggaaaatcaa catgcgtagt
     7021 cacaaaacat ccattctgat tatcaatact gagggaaaaa gaaacaaaac ccttcttgtt
     7081 ccaaccagga tacttctgca ctggaaaaaa tacgcagccg tagcggtgct tgtggtgctt
     7141 tgcctcattg ccgccctggg ggtgacggtg tttcacaaaa caagcaaaat ctaccaacat
     7201 gagatcgcca aggcagacaa gatcaagagc ctcgttgacg tccccaaagc gcaacagacc
     7261 ttccagtcga tcgatgaaag tattttccgt ataaacgaac tgctgaaatc gagggggctg
     7321 gtccagatgg aggttgagaa cctgggcggc ccggaaaacg tagaagtggt aaaaataaac
     7381 gagctggcgg acttttacag ggagcagctc gataacctgg agaagacact gaagaatgtt
     7441 cccatcggga accccacccc atccgacaag atcatttcat cctacggtta caggaggaac
     7501 ccgttcaccg gccgcggtgc agagtatcat tccgggatcg acatcaaggg cgaaaggggt
     7561 actccggtta aaacaacggc aaacggcaag gtagaagttg cgggatggta cggaggatac
     7621 ggaaaatgcg tgattgtaaa acacgaaaac aagctgaaaa cgctgtacgc ccatttatcg
     7681 gagataaccg tccgcgaagg cgatgagata gagagcggac aggttatcgg cagactcggg
     7741 agcacggggc gaagctcagg cccccacctg cattacgaga tcataaaaga cgacgagaaa
     7801 gtgaacccgg gcgactacat taattttcac gaatgaatat ccgatgagaa acagcaaaag
     7861 caaaagcaaa agcaaaagcg atcattccat tgagtcacaa ccgatagaca cggtgattgg
     7921 cacggatatc ctgttcaagg gagacatacg gggggaaagc atcatccgcg tcgatggaac
     7981 cgtggagggc aacctctccc tgtcgaaagg cattgttgtt ggggaaaaag ccaaggtgac
     8041 cgggaacctg aaaagcgacc gcatcatcat ctacggaagc atttccggcg acatcgaatg
     8101 caaagagttg aacatcaaaa gctcgggaac cgtgaacggc aacatccagg cagacaatgt
     8161 tgaaatagaa atgggaggaa agtacaacgg aaatctatcc atgcaaccct ctcccccccc
     8221 ttcatcggca aaagacaaag aagaccgctg aatccaacgg ttagtcgagc ttcagtaccg
     8281 ctaaaaacgc tttttgcgga acttccacgt tacctacctg cttcatgcgc ttcttccctt
     8341 ccttttgttt ttcgagcagc ttgcgcttac gggagatgtc cccaccgtag cacttggccg
     8401 ttacatcttt ccggactgcc tttacagtct cccgggcaat aatcttggcc ccgatcgccg
     8461 cctgtatggc gatatcgaac tgctgtcggg ggatcagttc tttcagcttc tcacacatcc
     8521 tgcgtccgaa agattgtgcg ttgtcggcat gggtgagtgt ggaaagtgca tccaccggct
     8581 ctccgttgag caggatatcg agcttcacca gtttcgaagg ccggaaatcg tggatgtggt
     8641 aatcgaacga cgcataccct ttggagatgc tcttcagctt atcgtaaaaa tcgataacga
     8701 tctctcccag cggcaagtcg aaaataatct ccacccggtc tccggaaatg tactcctgct
     8761 tgatgaggat accccgcttg cccaggcaca gggtcatgat cgggccgatg tatgtcgtgc
     8821 ttgtaatgat ggacgaacgg atataaggct cgtcgatatg gtcgatcagt gtgggatcgg
     8881 gcaatccggc ggggttgtgc acctccttca tgttgccttt cttgtcgtac accttgtacg
     8941 aaacgttggg aacggtggtg atcacgtcca tatcgaactc gcgctccagc cgctcctgaa
     9001 tgatttccat gtgcagcaat ccgagaaaac cgcagcggaa accgaatccc agggccaccg
     9061 acgattcggg ttggaaagtg agcgaagcat cattcagctg caacttttcc agcgatgccc
     9121 gcaagttttc aaactcctcg ctttcgatgg ggtaaacacc ggcaaaaacc attggcttca
     9181 cctcttcgaa gccggcgatg gctttgtcgc aggggttctt cacgtgcgtg atggtgtccc
     9241 ccacctttac ctctttcgac gttttaattc ccgagatgat ataccccacg ttgccgcatt
     9301 caacccgctg gcgggggtcc atgccaaggc gaaggatacc cacctcgtcg gcatcgtact
     9361 ctttcccggt gtttacgaac ttcaccaggt cacctttggc aatgctgccg ttcatgattt
     9421 tgaagtacgc aatgattccc cggaacggat t
//
LOCUS       DAOSDR010000064         9448 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrimonas sp. isolate AMR_MDS_1826
            NODE_2707_length_9448_cov_4.360694, whole genome shotgun sequence.
ACCESSION   DAOSDR010000064 DAOSDR010000000
VERSION     DAOSDR010000064.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863355
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrimonas sp. (wastewater metagenome)
  ORGANISM  Petrimonas sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Dysgonomonadaceae; Petrimonas.
REFERENCE   1  (bases 1 to 9448)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9448)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:10:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,720
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 35
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9448
                     /organism="Petrimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2707_length_9448_cov_4.360694"
                     /isolate="AMR_MDS_1826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2023866"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..256)
                     /locus_tag="PLI30_03905"
     CDS             complement(<1..256)
                     /locus_tag="PLI30_03905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019539435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HOI78732.1"
                     /translation="MLFQSHIGEIASILTAICWTLSALFFQRAGAKVGSLSVNIIRIF
                     LGILFLGVTTFFTRGMFFPMDATPYNWFWLGLSGVVGFFLG"
     gene            complement(325..1101)
                     /gene="trpA"
                     /locus_tag="PLI30_03910"
     CDS             complement(325..1101)
                     /gene="trpA"
                     /locus_tag="PLI30_03910"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018074756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HOI78733.1"
                     /translation="MNRIDELFQRKKNNILSVYFTAGFPNLSDTVPVIQSLEKNGVEL
                     IEIGIPFSDPMADGPVIQASGTQALKNGMSLRVLFEQLSRVRSSAQIPLVLMGYLNPI
                     LQFGFNTFCSEAQRCGIDGIIIPDLPFAEYMREYKAVAEKHGLRMIMLITPETSEERI
                     RLIDKNTGGFIYMVSSASVTGAKTGFGEDNLQYFKRVNAMNLRNPRLIGFGISNKITF
                     DAACEHASGAIIGSKFVSLLGSEKSVEAAVQKLKKSIANY"
     gene            complement(1107..1754)
                     /locus_tag="PLI30_03915"
     CDS             complement(1107..1754)
                     /locus_tag="PLI30_03915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018668373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HOI78734.1"
                     /translation="MKLVKVCGMREATNIREVEQAGADWIGFIFYPESPRFVHEVPDY
                     LPRKSKRVGVFVNAPKEKISETISLFGLDLVQLHGNESPGFCENIRRSGVKVIKSFRV
                     GDEFKEEEVEPYHGKCDYFLFDTQTSLYGGSGEKFNWETLLNYRGNTFFLLSGGITPG
                     DAEALKTFSYPKCIGVDINSKFEISPAVKDAKLVKQFIKVIKKPGNKIDNKTLLI"
     gene            complement(1767..2555)
                     /gene="trpC"
                     /locus_tag="PLI30_03920"
     CDS             complement(1767..2555)
                     /gene="trpC"
                     /locus_tag="PLI30_03920"
                     /EC_number="4.1.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019539438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004425 - indole-3-glycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="indole-3-glycerol phosphate synthase TrpC"
                     /protein_id="HOI78735.1"
                     /translation="MKDILNEIIAHKRNEIAAQKNSIPYSLLERKLADCHLPVYSLKD
                     ALLQSSTGIIAEFKRRSPSKGWINQHANAGNVTKGYEVAGATALSVLTDSTYFGGTLA
                     DLQHAARQAKIPVIRKEFIIDEYQILQARLSGASAILLIAAALSIEETHRFIRLSKQL
                     GLEVLLELHDEKEIEYTSPETTIIGINNRNLGTFVTDLKKSYRMVELLPRDAVWVSES
                     GISNPETVKELRKAGYRGFLIGENFMRSANPGASLEEFIKGVLQ"
     gene            complement(2565..3560)
                     /gene="trpD"
                     /locus_tag="PLI30_03925"
     CDS             complement(2565..3560)
                     /gene="trpD"
                     /locus_tag="PLI30_03925"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005778337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004048 - anthranilate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HOI78736.1"
                     /translation="MKHILVKLLDYQYLSRDESRELLIAIVRGEVPDTQVAALITIFL
                     MRSISVEEVLGFREALVEMRVPVDLKEYKAIDIVGTGGDGKNTFNISTSACFVVAGAG
                     YPVVKHGNYGATSVSGSSNAMEQQGVKFTTDIHVLRKSIEATNIAYLHAPFFSPALKA
                     VAPVRKSLGVRNFFNVLGPLINPSDPEYQLLGVYDLSMSRLYSYIYQASRKKFGIVHS
                     LDGYDEISLTGQFKVVTNKGEQLFMPEELGFNRVRQEEIFGGNTIEDVVSVFNRVLNC
                     TATQPQMDVVIANAAFAIQIIENKKPIAECIEIAKNSLYGKKALNALKKFVEINR"
     gene            complement(3557..4123)
                     /locus_tag="PLI30_03930"
     CDS             complement(3557..4123)
                     /locus_tag="PLI30_03930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008763094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HOI78737.1"
                     /translation="MKILIFDNYDSFTYNLRHVVRELGYTDIDVIRNDKIDLDDVDRY
                     DKIILSPGPGIPEEAGDLLPLIHRYAPGKSMLGVCLGHQAIGLSFGATLENTPLVHHG
                     VQTPIRMVGSDYIFDALPTRIEVGRYHSWIVSDKNFPSHLEITATDESGNIMALKHKT
                     LDVHGVQFHPESVLTPLGTEIIKNFLEK"
     gene            complement(4153..5559)
                     /locus_tag="PLI30_03935"
     CDS             complement(4153..5559)
                     /locus_tag="PLI30_03935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010800040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I family
                     protein"
                     /protein_id="HOI78738.1"
                     /translation="MTYKFTTNCKKVMGDLHTPVSLYLKIRDAFPQSTLLESSDYHAN
                     ENSTSYIGLNPMAHIDINRGECIETYPDGNTVRNRISGEFTISGALNNFINNFCINGD
                     NSSLTGLFGYTTFNCVKYTEGINVKESTEDRNDAPDMRYILYKYLMVFNHQKDELLLI
                     ELLGNGEESKLYKVESLINARNFASYDFFIHGQESSPITDDRFKSYVRQGIEHCHRGD
                     VFQIVLSRRFVQEFSGDDFKVYRALRTINPSPYLFYFDFGGFRIFGSSPETHCQIKGK
                     TISIDPIAGTAKRTGNLQEDAEATERLKSDPKENAEHVMLVDLARNDLSRNAHKVKLD
                     FYRQVQYYSHVIHLVSRVSGTLDEDVNPVQAFFDTFPAGTLSGAPKVRAMQLISEIEE
                     HNRGAYGGCIGFIGFNGDINQAITIRTFVSRNNQLWYQAGAGIVSQSIEENELQEVKN
                     KLGALKKAIDLAEKLNNV"
     assembly_gap    5632..5731
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(5773..6960)
                     /gene="trpB"
                     /locus_tag="PLI30_03940"
     CDS             complement(5773..6960)
                     /gene="trpB"
                     /locus_tag="PLI30_03940"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002560838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HOI78739.1"
                     /translation="MQNFSVDKSGFYGEFGGAYIPEILHRCVTELQDAYLRILESESF
                     KKDFDQLLRDYVGRPSPLYWSGYLSGKHGCSIYLKREDLNHTGAHKINNTVGQILVAK
                     RLGKTRIIAETGAGQHGVATATVCALMQMECIVYMGKTDVERQAVNVKKMQMLGATVV
                     PVTSGNMTLKDATNEAIRDWCCNPTDTHYIIGSTVGPHPYPDMVARLQSVISEEIRKQ
                     LMEKENRAVPDYLFACVGGGSNAAGTIYHFLNDDAVKIVLAEAGGKGIDSGETAATIQ
                     MGSIGIIHGCKTLVMQTEDGQITEPYSISAGLDYPGIGPIHAHLTTTRRAQVVAVNDD
                     EALKAAFELTRYEGIIPALESAHALAALDKITFKPSDIVVLTLSGRGDKDLETYFKQI
                     DAD"
     gene            complement(7218..8003)
                     /locus_tag="PLI30_03945"
     CDS             complement(7218..8003)
                     /locus_tag="PLI30_03945"
                     /inference="COORDINATES: protein motif:HMM:NF023521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3575 domain-containing protein"
                     /protein_id="HOI78740.1"
                     /translation="MKKTALLSLLALMIMGGVNAQQINRQSNAADGLLSGGKTILKAN
                     VLGWATRNFGFYGEQIISKNISAVLGVNIMPQGGVPYISRFSDDPQLEDIRIGTFSFT
                     PEVRFYLSRSGYGKGFYIAPYYKYERFKASNLLIEFEDQNSVPQEINLKGNLNTHSGG
                     AIIGIQWLAGKKKRIALDWTIIGAHYGTNKALFNGTSSHEMSAEEQASLKQEIIKNLN
                     DIEIGGKKIAEVERVDVSSTSAEAKTKGPWAFIRSSFSIGYRF"
     gene            8428..>9448
                     /locus_tag="PLI30_03950"
     CDS             8428..>9448
                     /locus_tag="PLI30_03950"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008774267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004333 - fumarate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0006106 - fumarate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006108 - malate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HOI78741.1"
                     /translation="MKYRVEKDTMGEVKVPADKLWGAQTARSLHNFKIGAPASMPLDI
                     IYGFAYLKKSAAYANCELGVLSQEKRDLIAQVCDEILEGKLDDQFPLVIWQTGSGTQS
                     NMNVNEVIANRAYQISGREVGDGEKPLHPNDDVNKSQSSNDTFPTAMSIACYKKIVEV
                     TLPGLRGLQRTLAVKSIETEEVVKIGRTHFMDATPLTLGQEFSGYAAQLEYGIKAIET
                     TLTHLAELALGGTAVGTGLNAPRNYDVLAAKYIAEFTGLPFVTAKNKFEALAAHDALV
                     ETHGALRQVAVSLNKIANDIRMLASGPRSGIGEITIPANEPGSSIMPGKVNPTQAEAV
                     TMVCAQ"
BASE COUNT         2312 a         2179 c         2222 g         2635 t
ORIGIN      
        1 cgcccagaaa gaagcccacc acgcccgaca agcccagcca gaaccagttg tacggagtgg
       61 catccatagg gaagaacatc ccgcgggtga agaacgtggt gactcccaga aacaaaattc
      121 ccaggaaaat ccggatgatg tttaccgaca gcgaaccgac tttcgctccc gctctttgaa
      181 aaaaaagggc gctcagcgtc cagcagattg ccgtcagtat cgaagcgatc tcgccaatgt
      241 gtgattgaaa aagcatgaaa tgtaatgtgg gctgtatttg tccgaaaaat catgcaaagg
      301 taggtgaata ttgagaattt cccattaata attggcaata gattttttca atttttgtac
      361 cgcagcctcc accgattttt cacttccgag caaggagacg aacttactgc cgataatggc
      421 tcccgaagca tgttcacaag ccgcatcgaa ggtgattttg ttggagatgc cgaacccgat
      481 aagcctgggg ttcctgaggt tcatagcgtt aacgcgctta aaatattgca ggttgtcttc
      541 cccgaaaccg gtttttgctc cggtaaccga tgccgacgag accatataga taaacccgcc
      601 agtatttttg tcaattaaac ggatgcgttc ttccgacgtt tccggagtga tgagcataat
      661 catacgcaat ccgtgttttt cggcaacggc tttatactcc ctcatgtatt cggcaaacgg
      721 taaatcggga atgatgatcc cgtcgatccc gcagcgttgc gcttcggagc agaatgtgtt
      781 aaagccgaat tgaagaatgg ggttcaggta acccatcaat accagaggga tctgcgccga
      841 cgagcggacc cgcgagagtt gctcgaagag cacccgaagg ctcatgccgt tcttgagtgc
      901 ctgtgtcccc gacgcctgga tgacgggacc gtctgccata gggtcgctga aagggatgcc
      961 tatttcaatc agttccacac cgtttttttc gagcgattga atgaccggaa cggtgtcact
     1021 gaggttgggg aatccggccg tgaaatagac ggataaaatg ttgttctttt tccgttgaaa
     1081 taactcatct attctgttca taattattaa attaataatg ttttgttgtc tatcttgttt
     1141 cccggttttt ttatgacttt aataaactgt ttaaccaact ttgcgtcttt aacagcgggg
     1201 ctgatttcaa atttactgtt gatatctact ccgatgcatt tcgggtacga gaaagttttt
     1261 aatgcttctg catcgcccgg tgttatgccg ccgctcagta aaaaaaacgt attaccacgg
     1321 taatttaaaa gagtctccca gttaaatttc tcccccgaac cgccgtacaa cgatgtttgg
     1381 gtatcgaaca gaaaatagtc gcatttgccg tgatagggct ccacctcttc ctccttgaat
     1441 tcatcgccga cccggaagga tttgattact tttaccccgc ttcggcgtat attctcacaa
     1501 aatccgggcg attcattgcc atgaagctgt accagatcca atccgaaaag agatatcgtt
     1561 tcactaattt tttctttcgg cgcgttcaca aaaactccaa ctcgtttgga tttcctgggt
     1621 aaatagtccg gaacctcatg cacaaaacgc ggtgattccg gataaaaaat aaatcctatc
     1681 caatccgctc ccgcttgttc tacttcgcgg atgttggttg cttcgcgcat cccgcatact
     1741 ttaactaatt tcatgtgtat attttgttat tgtagcactc cttttataaa ttcttccagg
     1801 cttgcaccgg gattagctga tcgcatgaag ttttctccga taagaaatcc ccggtatccg
     1861 gctttgcgta actcttttac cgtttctggg tttgaaattc cgctttccga tacccagacg
     1921 gcatcccgag gcaacaactc taccattctg tacgactttt tcaggtccgt tacgaaagta
     1981 cccaggttcc ggttgttgat gccgataatg gtggtttcgg gagaagtgta ttcaatttcc
     2041 ttctcatcat gaagctccag taaaacctcc aaacccagtt gtttcgataa ccggataaac
     2101 cgatgcgttt cttctatgct cagtgctgca gcaataagta agatggcgct tgcacccgat
     2161 aaccgggctt gcaggatctg gtattcgtct atgataaatt ctttgcgtat taccggtatt
     2221 tttgcctgtc gggctgcatg ctgcaaatcg gcaagcgtgc cgccaaaata ggttgaatcg
     2281 gtcagtaccg atagtgcagt ggctccggca acctcatatc ctttagttac attccctgcg
     2341 ttggcatgtt gatttatcca tcccttgctt ggcgaacggc gtttaaactc tgcaatgatt
     2401 cctgtactgg attgcagcaa cgcatctttg agggaataga cgggtaaatg gcagtctgcc
     2461 agtttccgtt ccagcaggga ataaggtatg ctgttttttt gtgccgctat ttcgttccgt
     2521 ttgtgggcga ttatttcatt tagaatatct ttcatatggg tggtttatct gtttatttcc
     2581 acgaatttct tgagcgcatt caatgccttt ttcccgtaca acgagttttt ggcaatctca
     2641 atacactccg caatcggttt tttgttttcg atgatctgaa tggcaaatgc ggcattggca
     2701 ataaccacat ccatctgggg ttgcgttgcc gtacagttga gtaccctgtt gaatacagaa
     2761 accacatctt cgattgtgtt tcctccgaaa atttcttcct gtctcacacg attaaacccc
     2821 agctcttcgg gcataaaaag ttgttctccc ttgttggtca ccactttgaa ttgcccggtg
     2881 agtgaaattt catcataacc gtccaggctg tgaacgatac cgaatttttt tcggcttgcc
     2941 tggtaaatat agctgtacag ccgcgacatc gataaatcgt agacgcccag caactgatac
     3001 tccggatcgg aagggttgat gagcggtccg agtacattga aaaagtttct cacgcccaga
     3061 cttttcctca ccggggcgac cgcttttagg gcagggctga agaaaggcgc gtgaaggtac
     3121 gcgatgttag tggcctcgat gctttttctc aggacgtgga tgtcggttgt gaatttgacc
     3181 ccttgctgtt ccatggcgtt gctcgagccg ctgaccgaag tggcaccgta atttccgtgt
     3241 ttcacaacgg ggtatccggc tccggcaact acaaaacagg ccgaagtgga aatgttgaac
     3301 gtgtttttcc catcgccacc agtacctaca atatcaatgg ccttgtactc ttttaggtcg
     3361 acgggaaccc gcatctccac cagcgcttcg cggaaaccga gcacttcttc taccgaaatg
     3421 ctgcgcatca gaaatatggt gataagtgcc gccacctgtg tgtcgggcac ttctcccctc
     3481 acgattgcta tcaataattc gcgcgattcg tcgcggctca ggtactgata atccagtagt
     3541 ttgacaagta tatgtttcat ttttctaaaa agttttttat gatttccgtg cccaacgggg
     3601 taaggacaga ttcgggatga aactgaacgc cgtgaacgtc taatgttttg tgcttcaaag
     3661 ccattatgtt gcccgattca tctgtggccg taatctccag atgggaggga aagtttttgt
     3721 ccgatacaat ccaggaatgg taccgcccca cttctatccg ggttgggagg gcatcgaaga
     3781 tataatcgct gcctaccatc ctgatgggtg tttgcacgcc gtggtgcacc aggggagtat
     3841 tctcgagggt ggcgccaaag gacaatccga tcgcctgatg ccccaggcaa acccccagca
     3901 tgcttttgcc cggcgcatat cggtggatta gcggtaacag gtctcctgct tcctccggaa
     3961 ttcccggacc gggtgaaaga atgattttgt cgtatctatc cacatcgtcc agatcgattt
     4021 tatcgttccg gataacgtct atatcggtgt acccaagctc ccggacgacg tgtcgcaggt
     4081 tgtaggtgaa ggaatcgtaa ttatcgaata tcagaatttt cattttttta agacagttag
     4141 acagttttag cttcacacat tattcaactt ctccgcgagg tcaattgctt tcttcaaggc
     4201 ccccagtttg tttttaactt cctggagctc gttctcttcg atgctctgcg atacaatccc
     4261 tgcgcctgcc tgataccaga gctggttatt ccggcttaca aacgtgcgga tggttatggc
     4321 ctggttgata tcgccgttaa atccgataaa cccgatgcag ccgccatatg ccccgcggtt
     4381 gtgttcctcg atctccgata tcagttgcat ggccctcacc ttgggtgctc ccgataatgt
     4441 cccggccggg aaggtatcga aaaacgcctg gacgggatta acgtcctcat cgagcgtgcc
     4501 gcttacgcgg gagaccaaat gaatgacatg cgaataatac tgtacttgcc ggtaaaaatc
     4561 gagttttact ttgtgtgcgt ttcggctcag gtcgttccgg gccagatcga ctagcatcac
     4621 gtgttcggca ttttctttcg ggtcgctttt gaggcgttcg gtagcttccg cgtcctcctg
     4681 aaggttccct gttcgcttag ccgttccggc aatagggtct atgctgatgg ttttaccctt
     4741 gatctgacaa tgtgtttcgg gtgaagaacc gaaaatgcga aaaccgccga aatcaaagta
     4801 gaagagatag ggggacgggt tgatggttcg cagtgcgcgg taaaccttaa agtcgtctcc
     4861 cgaaaattcc tgcacgaagc gtcgcgagag aacgatctga aacacatcgc cccggtggca
     4921 gtgttcaatg ccctgccgga cataagactt gaaccggtcg tcggtaatgg ggctcgattc
     4981 ctgaccgtga ataaaaaagt cgtacgatgc aaaattccgg gcattgataa ggctctccac
     5041 cttgtagagt ttgctttcct ctccattgcc aagcagttcg atcagcagca gttcgtcttt
     5101 ctgatgatta aaaaccatca ggtatttata gaggatatac cgcatgtcgg gggcgtcgtt
     5161 acggtcctcg gtactctcct tgacgttgat cccctcggta tatttcacac agttgaacgt
     5221 agtatagcca aaaagccccg tcagggagga gttgtcgccg tttatgcaga agttgttgat
     5281 gaaattgttt aatgccccgg aaatggtaaa ctctccggat atcctgttcc tgacggtgtt
     5341 cccgtcggga taggtttcga tgcactcgcc ccggtttatg tcgatatgcg ccatgggatt
     5401 cagcccgatg tacgacgtgc tgttttcgtt ggcatgataa tcggaacttt cgagcaatgt
     5461 cgattgggga aaggcatccc ggattttcag ataaaggctt accggtgtat gcaggtcgcc
     5521 catgaccttt ttgcagtttg ttgtgaattt gtaagtcata ttttttagat tgaaagagat
     5581 tgaaagagat tgaaagagat tgaaagagat tgaaagagat tgaaagagat tnnnnnnnnn
     5641 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5701 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ngatttccag actgtataca acaacctgtt
     5761 ttcaatctta aatcaatctg catcaatctg tttaaagtaa gtctccaggt ctttatcccc
     5821 ccttcccgaa agtgtcagga caactatgtc ggacggtttg aaagtgattt tatcgagcgc
     5881 agcgagtgcg tgagccgact ccaatgcagg gataatgcct tcataacgcg taagttcgaa
     5941 agcggctttt agggcttcgt catcgtttac ggcaactacc tgtgcccgcc gggtagttgt
     6001 caggtgggcg tggatgggcc caatacccgg atagtccagc ccggcggaaa tggaataagg
     6061 ttccgtgatt tgtccgtctt ccgtttgcat caccaacgtt ttgcacccgt ggataattcc
     6121 aatagagccc atttgtatgg tggctgccgt ttctccgctg tcgattccct tgccgcctgc
     6181 ttcggcgagt acgattttca ccgcatcatc gttcaggaag tgatagatgg ttcccgcagc
     6241 gttgcttccc cctcccacgc aggcgaaaag ataatcggga acggcacggt tttccttttc
     6301 catcagctgc ttcctgatct cttcactgat gactgattgt aaccgggcta ccatatcggg
     6361 ataggggtga ggcccaacgg tggatccgat gatataatgt gtgtccgtcg ggttgcagca
     6421 ccagtcgcgg atggcttcgt tggtagcatc cttcaacgtc atgttccccg aagtaaccgg
     6481 aacgaccgtt gcacccagca tctgcatctt cttgacgttt acggcttggc gttccacatc
     6541 ggtttttccc atataaacaa tacattccat ctgcatcaaa gcacaaacag tagcggttgc
     6601 cacaccgtgc tgcccggcac ccgtctcggc gataattcgg gttttcccca atcgtttagc
     6661 aacaagaatc tggccgactg tattgttgat cttgtgtgcc ccggtatggt ttaagtcttc
     6721 ccgtttgagg tagatgctgc atccgtgttt accggaaagg tacccagacc agtaaagcgg
     6781 agaaggcctg cctacgtaat cccggagaag ctggtcaaaa tcttttttaa acgattcgct
     6841 ttccaggatt ctgagatagg cgtcctgcaa ttcggtaacg catctgtgaa ggatttcagg
     6901 aatataggct ccgccaaatt caccgtaaaa tccggactta tctactgaaa aattctgcat
     6961 atactgtttt ttttgtttga taccaatgaa aagagcctgt cgcaggtgcg acaggctctt
     7021 ctatgtttat tgttattgag ttcttgctat agatacgact gcttccctta cgaccggttg
     7081 agttgtaagt agcgccacca atatccgtta gcaagagaaa tgttcatatt ccaattattt
     7141 tgctgcaaat gtaggtttaa aaaaataaaa agcaatgtat ttttcaaact ttaacattta
     7201 aaaaatgttt cttacattta aaacctgtat cctatggaga aagaagagcg gataaaagcc
     7261 cacgggcctt tggtctttgc ttctgccgaa gtggaagaga catcaacacg ctctacctcc
     7321 gcaatttttt tccctccaat ttcaatgtcg ttcaggttct ttataatctc ctgtttcagg
     7381 cttgcttgct cttctgcaga catttcatgg ctggaagttc cgttaaatag ggctttattc
     7441 gtgccgtagt gagcaccaat gattgtccag tcaagcgcga tccttttctt tttcccggct
     7501 aaccattgta ttccgataat tgctccccca ctgtgggtat tcaaattacc cttaaggtta
     7561 atttcttgcg gcacactatt ctgatcttca aattctatta agaggtttga agctttaaag
     7621 cgttcgtatt tgtaatatgg ggcaatatag aagcctttac cgtagccgga tctgcttaag
     7681 taaaaacgca cctcgggtgt aaaggagaac gtgccaatcc ggatgtcttc gagttgagga
     7741 tcatctgaaa aacggctgat ataagggacc ccgccctggg gcataatgtt cactcccagt
     7801 acggcgctga tgtttttgct aatgatctgt tctccgtaaa acccgaaatt ccgagtcgcc
     7861 caccccagga cattggcctt taggatggtt ttgcctcccg acagcagccc gtcagcggca
     7921 ttcgattgtc tgttaatttg ttgtgcgttg acaccaccca taatcatcag tgccaacagg
     7981 gataataaag ctgttttttt catgtgagtg ttattaaaaa gtgataccgg gattttaccg
     8041 caaaaacagg gaaatatttt tgtaagaaat aaacgaaaat cccggcaaat tgttcaataa
     8101 ttttatatta ttttttataa taaaaaggta tagctgttaa acttgaattt ggtctgttga
     8161 gtaaaaaatt gtttctttgc tacagcgtgc ggtctatttg gttcagtgat atacagaaga
     8221 tgtttccatc ctgattatct actcgcatta ataaggttgg acgctgtttg gttatcaggc
     8281 tttgaaggaa agggcaggtc tgccttttta ctgacttggg gaatgaggca caatatatcg
     8341 tctgttggaa gatttggccg tagcagtaac gggtacaaaa gttaaataaa gaaaatcaga
     8401 aaagggatga attctaaaaa atccaaaata aaatacagag ttgagaaaga tacgatgggg
     8461 gaggtaaaag ttcctgctga taaactctgg ggtgcgcaaa ccgcacgttc gctccacaat
     8521 tttaaaatag gagcgcctgc ttccatgcct ctggatatta tttatggatt tgcgtacctg
     8581 aaaaaaagtg ctgcgtacgc caattgcgag ctgggggtgt tgtcgcagga aaaacgggac
     8641 ctgattgcgc aggtgtgtga tgaaatactg gaggggaagc tggacgatca gttccctttg
     8701 gttatctggc agaccggctc tggaacgcaa agcaacatga acgtaaacga agtgattgcc
     8761 aaccgcgctt atcagatttc cgggagggaa gtcggcgatg gagagaagcc ccttcacccg
     8821 aacgatgatg taaataaatc gcagtcatcc aacgatacgt ttccaacggc gatgtccatt
     8881 gcctgctata aaaaaattgt tgaggttacc ttgccgggat tgcgtggttt acaaagaaca
     8941 cttgctgtta aatccatcga aacggaagaa gtggtcaaga tcgggcgtac tcactttatg
     9001 gatgctaccc ccctgacatt gggacaagag ttcagtggat atgctgctca gctggaatac
     9061 ggtataaaag ccattgaaac caccttgact cacctggccg aactggctct tggcggaacc
     9121 gcggtaggga ccggcctgaa tgctcccaga aattacgatg tcctcgcagc aaaatacata
     9181 gcggagttta ccgggttgcc tttcgtgact gcaaaaaata aattcgaagc acttgccgca
     9241 cacgatgccc tggttgaaac gcacggagca ttgagacagg tggcggtttc gcttaacaag
     9301 atagccaatg atatccgtat gcttgcatcc gggccacgaa gtggaattgg agagataacg
     9361 atccctgcca atgagccggg ttcttccatc atgccgggga aggtgaatcc tactcaggca
     9421 gaagctgtaa cgatggtttg cgcacaaa
//
LOCUS       DAOSDR010000065         9355 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrimonas sp. isolate AMR_MDS_1826
            NODE_2740_length_9355_cov_3.648817, whole genome shotgun sequence.
ACCESSION   DAOSDR010000065 DAOSDR010000000
VERSION     DAOSDR010000065.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863355
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrimonas sp. (wastewater metagenome)
  ORGANISM  Petrimonas sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Dysgonomonadaceae; Petrimonas.
REFERENCE   1  (bases 1 to 9355)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9355)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:10:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,720
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 35
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9355
                     /organism="Petrimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2740_length_9355_cov_3.648817"
                     /isolate="AMR_MDS_1826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2023866"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1656
                     /locus_tag="PLI30_03955"
     CDS             <1..1656
                     /locus_tag="PLI30_03955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009276142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HOI78742.1"
                     /translation="GLAFDGIVDVLKQKLYKWKPGATAPDVLEIPDSEKEKAGEYYQV
                     LLEAAAENDETLMEKYFDQGTLSEEEMLEGIQKGMISRDLFPVFCVSAEKNMAVHRLM
                     NFLTLAAPSPDQVPSPQNSDGETVEPDPGALTSLFFFKTTVEPHIGEVSYFKVMSGKV
                     KEGDDLTNTNRSSKERIAQMYLVAGQMRNKVEELQAGSIAAAVKLKDVRTGNTLNAKG
                     SDNRFNFIQFPEPRYRRAVKARTEANSEKLSEALTRMREEDPSLVIEVSKELKQTIVS
                     GQGEFHLKTMKWRLENNDKIEIDFLEPKIPYRETITKQSRADYRHKKQSGGAGQFGEV
                     HLIVEPYTEGMPMPEIYRFNGQEFKIQSRDVQTIDLDWGGKLVFVNSIVGGAIDARFL
                     PAILKGIMGRMEQGPLTGSYARDVRVIVYDGKMHPVDSNEISFMLAGRNAFSTAFKNA
                     GPKILEPIYDVVVSVPSDYMGDVMSDLQGRRAMIMGMESEKGFEKLKARVPLKEMSSY
                     STSLSSLTGGRASFTMKFSEYELVPSDVQDKLLKEYEAEEKEE"
     gene            complement(1759..2388)
                     /locus_tag="PLI30_03960"
     CDS             complement(1759..2388)
                     /locus_tag="PLI30_03960"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HOI78743.1"
                     /translation="MNYKHIIFDIDGTMLNTETADMLATQETFFQLTGEKRELDEFRF
                     SFGIPNSVVFPRLGIEDAGKASLMWAENYRKYHSSIRLFDGIEEVLKELKKRGYILGI
                     VTSKSRQEFNADFATFGLEHFFGTVICVDDSPRPKPHPDPILEYLKRSNAKKPESIYI
                     GDTLYDEQCAHSAGIDFALAVWGTHNREEIKADYFLEAPLEILELFRSR"
     gene            2462..3265
                     /locus_tag="PLI30_03965"
     CDS             2462..3265
                     /locus_tag="PLI30_03965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013632606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HOI78744.1"
                     /translation="MQKTIFHIKEMDCPSEEELVRMKLDEIPDIARIDINIQDRKATI
                     FHRANTAEIETLLNELKLGSSLVSSEVVEEKLSGADENKNRKQKKILWTVLFINFGFF
                     VIEMGTGLVSRSMGLVADSLDMLADAFMYAISLFAVGKAVSTKKKVAGAIGYFQILLA
                     STGLFEVARRFIFNESTPDFQFMIGISILALAANGISLYILEKAKSNEAHIRASMICT
                     SNDVIMNLGVIVGGILVFLLDSAIPDLVVGVVVFGVVIRGALRILKLAK"
     gene            3326..4054
                     /locus_tag="PLI30_03970"
     CDS             3326..4054
                     /locus_tag="PLI30_03970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI78745.1"
                     /translation="MTQADNMKKYFTVLILLSLSLSLLSQDFDGNYKDKTDSFSFSNG
                     QALFSLSGFGALFTRMVGEGKYEYVDNYLLVHTSGYPGEKSVFESINGTKKDSIVVKV
                     VGPDNYPVQGALTEFLSESNKIIRGNITDDLGKSQQPKDQKIKRIKVTNLGYDDVAFE
                     VEQGKDFLVKLARNNVIENQTVAFKVKREDEETLSIILLSDNFDPGKDKIKSLEKLDK
                     KTQKNNVLAKRLKKEYIPAYQGRE"
     gene            complement(4059..5045)
                     /locus_tag="PLI30_03975"
     CDS             complement(4059..5045)
                     /locus_tag="PLI30_03975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019538930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HOI78746.1"
                     /translation="MKKIVVCCVVVLWFTAVEAQKLNFNKDGLFKIAQFTDVHYVPGN
                     SKSDTALLAIQRVLDTEKPDMVIFTGDIVTGRPIKTGWDTVTKLVVDRKIPFAVALGN
                     HDDESGTTRLELEKIITDYPYNCNYPAGGQLSGVMNNVLPVYGSEKDMQVKALIYCFD
                     SGAYSTVSDVSGYGWITSEVIDWYKKQSFHFTRQNNFQPLPALAYFHIPLPEYRLAFD
                     DDTNKRVGVRKENECSPGLNSGMFLAMKEMQDVMGTFAGHDHVNNYIVNYFGIALAYG
                     QFSGWRTTYVPGINGARIVQLTEGKREFDTWIRLLDGSVEYNVTFPAGLKGE"
     gene            complement(5053..5988)
                     /locus_tag="PLI30_03980"
     CDS             complement(5053..5988)
                     /locus_tag="PLI30_03980"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009123405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009165 - nucleotide biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HOI78747.1"
                     /translation="MQDKPFKIFSGTKSRYFAEKVCNSLGCPLGNMIIEHFADGEFSV
                     SYEESIRGRQVFLIQSTFPNSDNLMELLLMIDAAKRASAKSIIAVIPYFGWARQDRKD
                     KPRVSIGAKLIADMLSTAGISRLITMDLHADQIQGFFDVPVDHLYGSGVFVDYMKTFN
                     QENLVIATPDVGGTKRASAYAKFLGCPMVICYKIRKKANVISDMQIIGDVEGMDVLLI
                     DDIVDTAGTMTKAAELMMQNGARSVRAIASHAVMSDPASERVENSSLNEIIFTDSIPY
                     TKSCSKVKVLSVADMFANAIMRVCNNESISSLYVV"
     gene            complement(6208..7905)
                     /locus_tag="PLI30_03985"
     CDS             complement(6208..7905)
                     /locus_tag="PLI30_03985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005932002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family protein"
                     /protein_id="HOI78748.1"
                     /translation="MKPRKIFIYQLLPRLLGNTQANNIQNGTLEENGCGKFNDITGKF
                     LNQLKKGGYSHVWLIGVLAHASTTDYTQYGIPREYPEIIKGNAGSPYAVRDVYDVDPD
                     LAMNVNGRMREFEALVERIHRAGMEVIIDFVPNHLARNYKSINRPEGVDEFGCNDDTS
                     VAFSPDNNFYYLPGEELEIQFPVKKMADVTYRESPAKVTGNDCFTNKPTPYDWYETVK
                     LNYGIDIQNGYIKHFSKIPDTWNKMRDVLIYWSKKNIDGFRVDMAEMVPLEFWRWVIP
                     QVKKEFPKILFLAEIYNPDAYRLFLAHDNFDYLYDKVGLYDVLRDVACGYRPSSDITF
                     ALNNVGDIQHKMLNFIENHDEQRVASDYFLKDGKHGPAAMIVTACVNVNPVMIYFGQE
                     LGERGMDEEGFSGKNGRTSIFDYWSLDTVRRWNNNGKWDDTLLTGEERALQKFYAKLL
                     TLCNRERALSEGLFYDLMPANYDNFEFDSTKQFAFLRGTGDELILAVVNFDNKEVDVV
                     VNIPTHAMDFFGIPDNGSFNAFPLLSDSKFNTVFSIDSPIRIKVGATSGELYKISSC"
     gene            complement(7902..8768)
                     /locus_tag="PLI30_03990"
     CDS             complement(7902..8768)
                     /locus_tag="PLI30_03990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019540623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HOI78749.1"
                     /translation="MAIFGKNFQYYLGYALSGGGAKGFAHLGAFKVFESYGLKPDVIA
                     GTSAGALAGVFYADGFSPDEISELFRKKEFHEFIGFSIPKTGFFKTSGVGKFLKDNLR
                     SSSFEQLRIPFKAVVTDWEKARTVVFSEGRSLVDAVVASCSVPIVFTPKVIGGVPYVD
                     GGLLKNFPVSVIRNECKYVIGVNVSLMTPLSDKVNIKTTAERTFKLMSNSNTLVDRSL
                     CDILVEAEEAQKYFMFDLNNIDKIREIGYECASTALGEEKSLGIIRRCHRHYELEEKV
                     RQRIGKFKKTTL"
     gene            complement(8966..>9355)
                     /locus_tag="PLI30_03995"
     CDS             complement(8966..>9355)
                     /locus_tag="PLI30_03995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI78750.1"
                     /translation="RDLYLVNRESTSSTDYVLKVRGKGTYTIDTEEGNCFMTFSYGVD
                     EKGQPTLDAESAKTVSYRFRMSINDLALHRLNQNLNLGLETSMPDNWKELIRYDWEIG
                     IPMEGMGTAYKAFGTLLSSFEMPVGVL"
BASE COUNT         2655 a         2225 c         1972 g         2503 t
ORIGIN      
        1 ggcctggcat tcgacggaat agtggatgtt ctcaagcaga agctgtataa atggaagccg
       61 ggagccaccg cgcccgacgt gctggagata cccgacagtg aaaaagaaaa agctggcgaa
      121 tattatcaag tattgttgga agcagcagct gaaaacgatg agacattgat ggaaaaatat
      181 ttcgatcagg gcacactgtc cgaagaagag atgctcgaag gcatccagaa agggatgatc
      241 agccgggatc tttttccggt gttctgtgta tcggcagaaa aaaacatggc ggtccacagg
      301 ctgatgaatt tccttaccct ggcggcaccc tcacccgatc aggttccgtc tcctcaaaac
      361 agcgacggag aaaccgtaga gcccgatcca ggagcactga caagcctgtt cttcttcaaa
      421 actactgttg aaccgcacat cggcgaagtt tcttacttca aggtgatgag cggaaaagta
      481 aaagaagggg atgacctgac caataccaac agaagttcga aggaacgtat cgctcagatg
      541 tacctggttg ccggacagat gcgtaacaag gtggaagaac tgcaggcagg aagcattgcc
      601 gctgctgtca agctgaaaga tgtccgtacc ggcaatacac tgaatgcaaa aggatcggac
      661 aaccggttca actttatcca gttccccgaa ccgcgttacc gcagggctgt aaaagctaga
      721 acggaagcca actccgaaaa actgagcgaa gcattgaccc gtatgcggga agaagatccg
      781 tcattggtta ttgaagtttc aaaagaactg aaacaaacca tcgtttcagg acaaggcgag
      841 ttccacctga aaaccatgaa atggagactc gaaaacaacg ataaaattga aattgatttc
      901 ctcgaaccta agattccgta tcgcgaaacc atcaccaaac agtcccgtgc cgattaccgc
      961 cataaaaaac agtcgggtgg tgccggtcag ttcggcgaag ttcacctgat tgtggaaccc
     1021 tacaccgaag gtatgcccat gccggaaatt taccgtttta acggccagga attcaagata
     1081 caatcgcgcg acgtacaaac tatcgacctt gattggggtg gaaagcttgt tttcgtgaac
     1141 agtatcgtgg gcggtgcgat cgatgcccgt ttcctcccgg ccatcctgaa aggaatcatg
     1201 ggacgtatgg agcaaggccc gctgaccggc tcttatgccc gtgacgtacg tgttatcgtt
     1261 tacgacggta agatgcaccc ggttgactcc aacgaaatct cttttatgct tgccggacgc
     1321 aacgcgttca gcaccgcatt taaaaatgcc gggccaaaga ttctggaacc gatttacgat
     1381 gtggttgttt ctgttcccag cgactatatg ggcgatgtga tgagcgacct gcaagggcgt
     1441 cgtgccatga tcatgggtat ggaaagcgag aaagggtttg aaaaactgaa agcccgcgtt
     1501 ccgttgaaag agatgtcgag ttattccact tctttaagtt cgcttaccgg tggccgtgcg
     1561 tcgttcacca tgaagttttc ggaatatgaa ctcgttccgt ccgatgttca ggataagctc
     1621 ctgaaagaat acgaggcgga agaaaaggaa gagtaaaaac ccggggagaa tctccaatat
     1681 tttacaggag ggtgtatcac aatgtgatta caccctcatt ctattcggat acctctctaa
     1741 gaatttccaa agaatgagct atctggatcg gaacaactcc aatatttcga gaggtgcttc
     1801 caggaaatag tcggctttta tctcttcccg gttgtgtgtt ccccaaacag caagggcaaa
     1861 atctatcccg gcagaatgtg cgcactgctc atcgtaaagg gtatcaccta tataaatgga
     1921 ttccggtttt tttgcattcg accgcttaag gtattccaga atagggtctg gatggggctt
     1981 tgggcgggga ctgtcatcca cacaaataac cgtaccaaag aaatgctcca acccgaaagt
     2041 agcaaaatca gcattaaatt cctgccggct tttggaagta acaattccca ggatgtatcc
     2101 cctttttttc aactccttca aaacctcttc aattccatcg aaaaggcgta tggatgagtg
     2161 gtatttacga taattttcgg cccacatcaa gctcgctttt ccggcatctt caattcccag
     2221 ccggggaaac actaccgagt tgggaatacc aaatgaaaac cggaattcat ccaactctct
     2281 tttctctccg gtaagctgaa aaaaagtttc ctgcgtggca agcatgtctg ccgtttccgt
     2341 attcagcata gtgccgtcta tgtcaaatat aatgtgtttg tagttcattt ctcaaatttg
     2401 ggggacaaag atacaaaaca aaaccgttct ccgagcgtta gagacatgaa acataacagc
     2461 tatgcaaaaa acaatatttc atatcaagga gatggattgt ccgtctgaag aagaactggt
     2521 ccgcatgaaa ctggacgaaa ttccggatat tgccagaatt gacatcaata tacaagatcg
     2581 aaaagcaacc atttttcacc gggccaacac ggccgaaata gaaacgctgc tgaacgagtt
     2641 aaaactcggc tcgtcgcttg tttcttcgga agttgttgaa gagaaacttt ccggcgcaga
     2701 cgagaataaa aacagaaaac agaaaaaaat tctctggaca gtgcttttta tcaacttcgg
     2761 attcttcgtt attgaaatgg gcaccggcct cgtttcccgt tcgatggggc tagttgccga
     2821 cagcctggat atgcttgccg acgcttttat gtatgccatc agcctatttg ctgttggcaa
     2881 agccgtaagc acgaagaaaa aagttgccgg agccattggt tatttccaaa ttttacttgc
     2941 ctcaaccgga ttgtttgaag ttgcaaggcg tttcattttc aacgaatcca ctcccgactt
     3001 tcaatttatg atcggcatct ctattcttgc acttgccgca aatggaatca gcctttacat
     3061 cctggaaaaa gccaaaagca atgaagcaca catccgggca agcatgatat gcacatcaaa
     3121 tgatgtaatc atgaacctgg gcgtcattgt gggtggaatc ctggtttttt tactcgattc
     3181 tgccattcct gatcttgttg taggtgttgt cgtctttgga gttgttataa gaggggcgct
     3241 ccggatactg aaactggcaa aatagtcggg gatttaacac tttccgacca taaaaaatgt
     3301 tatttttgta tcgttataca aatttatgac tcaggctgac aacatgaaaa aatattttac
     3361 cgttcttatt ttactatccc tatcgctttc tcttctgtct caagactttg atggaaacta
     3421 caaggataag accgattcat tctctttttc caacggacaa gcattgttta gcctaagcgg
     3481 ttttggagcc ctgtttaccc gaatggttgg cgaaggaaaa tatgaatatg ttgataatta
     3541 tttgctggta cacacttccg gctatcccgg tgaaaaatcg gtgttcgaat ccatcaacgg
     3601 tacgaaaaaa gactccatcg ttgtaaaggt agtcggcccg gataattacc ctgtccaggg
     3661 agcgctgacg gagtttctgt cggaatccaa caaaattatc cgtggaaata ttacagatga
     3721 cctgggcaaa tcccaacagc ccaaagatca aaaaattaaa aggataaagg taaccaacct
     3781 gggttacgat gatgttgcct ttgaggttga gcagggaaaa gattttttag taaaacttgc
     3841 cagaaacaac gttattgaaa accagacggt tgctttcaaa gtcaagagag aagatgaaga
     3901 aactctttcg atcatcctgc tttccgataa ctttgacccg gggaaagaca aaataaagtc
     3961 cctggaaaag ctcgataaga aaacacaaaa aaacaacgtg cttgccaaac gactaaagaa
     4021 agagtatata ccggcctatc aaggacgaga ataaaatttc actccccctt cagtccagcc
     4081 ggaaaagtta cattgtattc gacgcttccg tcgagtaacc tgatccaggt gtcaaattca
     4141 cgttttccct ccgtcagttg tacgattctc gcaccgttga ttccaggtac ataagtggtt
     4201 ctccatcccg agaactggcc gtacgctaat gcaataccga aatagttcac gatataattg
     4261 ttaacgtgat cgtgcccggc aaatgtaccc ataacatcct gcatctcttt catcgcgaga
     4321 aacatccccg agtttaaacc gggggaacat tcgttttcct tacgcacccc cacccttttg
     4381 ttcgtatcgt cgtcaaatgc caggcggtat tccggaaggg gaatatggaa ataagccagg
     4441 gctggcaacg gctgaaaatt attttgccgg gtaaaatgaa aactttgctt cttataccag
     4501 tcgatgacct cagaggttat ccatccgtat cccgacacat cgcttacggt tgaataggct
     4561 cccgagtcga agcagtaaat caacgctttc acctgcatat ctttttcgct tccgtaaacg
     4621 ggaaggacat tattcataac gccgctcaac tgtccaccgg caggatagtt gcaattgtac
     4681 gggtaatccg tgataatctt ttcgagttcg aggcgggttg tgccgctctc atcgtcgtga
     4741 tttcccaatg caacggcaaa cggtattttc ctgtcgacaa ctaattttgt aacggtgtcc
     4801 catcctgttt ttatcggcct tccggttaca atatccccgg tgaaaataac catatcgggc
     4861 ttttccgtat ccaatacgcg ctgaatagcc aacaaggccg tatccgattt ggaatttccg
     4921 gggacgtagt gcacatcagt aaactgtgct atcttgaaca aaccgtcctt gttgaaattt
     4981 aatttttgtg cttctactgc agtgaaccac aacacaacaa cacaacaaac aacaattttc
     5041 ttcatactct tgctatacaa catacaatga acttatcgac tcgttgttgc acaccctcat
     5101 gatggcattg gcaaacatat cggcgacaga caagactttc actttactgc acgattttgt
     5161 ataaggaata ctgtcggtaa atataatctc gttaagcgaa gaattttcca cacgttcact
     5221 ggccggatcc gacatgacgg cgtgactggc tatggctctt acagatctgg cgccgttctg
     5281 catcatcaac tcggcagctt tggtcatggt tcccgcagta tccacgatat cgtcaatcaa
     5341 caacacatcc atcccttcca catccccgat gatctgcata tccgaaataa cgtttgcttt
     5401 tttcctgatc ttatagcaga taaccatggg acaacccaag aacttggcat aagcactggc
     5461 acgcttggtt ccacctacat cgggcgtggc aatcaccaaa ttttcctgat taaaggtttt
     5521 catataatcc acaaaaactc ccgatccgta caaatggtca acaggaacat cgaagaaacc
     5581 ctgaatctgg tcggcatgca agtccatggt aatcaatcgg ctgatgccgg cagtgctgag
     5641 catatcggca atcagcttcg ctccgatact cacacggggc ttatcttttc tgtcctgacg
     5701 ggcccatccg aaatagggaa taaccgcaat gatggacttc gccgacgcac gcttggccgc
     5761 atcaatcata agcagtaact ccatcaggtt gtctgaattg ggaaaagtgg actggataag
     5821 gaatacttgt cttccccgaa tggattcctc gtatgaaact gaaaattctc cgtcggcaaa
     5881 atgctcgata atcatatttc ctaacggaca ccccaaacta ttgcaaacct tctcggcaaa
     5941 ataacgtgat tttgttcccg agaagatttt aaacggctta tcttgcatga cttgttgatt
     6001 ataattgagt tagtaaatta ttggttgatt ctctctttgt aatagtcttt ccacggacaa
     6061 aagtacaaac taaattctaa aaaaaccttt aaaatgtgtt ttgaattttt ttatgtactg
     6121 tttttagtca ttttttgatg gatttggatt ttcgtttcgc atcctatggc aggatatacg
     6181 aaagaaagga aagttcaaaa tactttctta acaagaggaa attttgtaca gctcgcccga
     6241 ggttgcccca acctttatcc gaatcgggct atctatcgaa aaaacggtat tgaacttgct
     6301 atccgacaac aacggaaaag cgttaaaact cccgttgtca ggaatcccga agaaatccat
     6361 agcgtgggtg ggaatattta ccacaacatc aacctccttg ttgtcaaaat taaccaccgc
     6421 cagaattaat tcatctccag taccccgtag aaatgcgaat tgcttggtgg aatcaaactc
     6481 aaaattgtcg taatttgctg gcatcaggtc gtaaaagagt ccctcactca acgctctctc
     6541 ccggttacac aaggtaagga gtttggcata aaatttctgc aacgcccttt cttctcctgt
     6601 caacaaggta tcgtcccatt tcccgttatt attccaacgc ctaaccgtgt ctagcgacca
     6661 ataatcaaaa atggaggtac gcccgttctt gccactgaaa ccctcttcat ccatcccccg
     6721 ctcgcccagc tcctggccaa aatagatcat tacaggattt acgttcacgc aggcagtaac
     6781 aatcatcgca gcgggtccat gctttccgtc tttcagaaaa taatccgaag ctacgcgttg
     6841 ttcatcgtgg ttttcaataa aattgagcat tttgtgctga atatcgccca cgttattcag
     6901 ggcaaacgtg atgtccgacg acggacgata cccgcaggcc acatcacgca aaacgtcata
     6961 taaccctact ttatcgtaca agtaatcaaa gttgtcgtgg gcaagaaaca accggtaggc
     7021 atcgggattg tatatctccg ccagaaagag aattttcggg aactcttttt ttacttgcgg
     7081 aatcacccat cgccaaaact ccagcgggac catttctgcc atgtctaccc ggaaaccgtc
     7141 aatatttttc ttcgaccaat agatcaatac gtccctcatc ttgttccacg tatcgggaat
     7201 tttggagaag tgttttatgt aaccgttctg tatatcgatg ccgtagttaa gcttaaccgt
     7261 ctcataccag tcgtatgggg tgggtttatt ggtaaaacaa tcgttcccgg tgacttttgc
     7321 cggcgactcc ctgtaggtta cgtccgccat tttctttacc gggaattgaa tttccagctc
     7381 ctcacccgga aggtaataaa agttgttgtc gggagaaaaa gccacagaag tgtcgtcgtt
     7441 gcaaccaaat tcgtcaactc cctccggacg gttaatcgat ttgtagttcc ttgccaggtg
     7501 attcggcaca aaatcaatga ttacctccat cccggcacga tggatacgtt caaccaacgc
     7561 ttcaaattcg cgcatccggc cgttcacgtt catcgccaga tcaggatcaa catcgtaaac
     7621 gtcccgcacg gcgtacggag acccggcatt gcctttaatg atttccggat attcacgggg
     7681 aatgccgtat tgggtgtaat ccgttgttga ggcatgcgcc aatacgccga taagccagac
     7741 atgagaataa ccacccttct ttaactgatt gagaaactta cctgttatat cgttaaactt
     7801 tccgcatccg ttttcttcca gggtcccgtt ttgaatgttg ttggcctgcg tgttccccag
     7861 cagacgcggc aacagctgat agatgaatat tttacgtggc ttcataatgt tgttttttta
     7921 aatttaccta ttcgctgcct cactttctcc tcgagttcgt aatgtcggtg acaccttctg
     7981 ataatgccaa gcgatttttc ttcaccaagc gctgttgaag cacactcgta gcctatttcc
     8041 cttattttgt cgatgttgtt caaatcgaac ataaagtact tttgcgcttc ctccgcttct
     8101 acaaggatgt cacataaact cctatctacc aacgtatttg aattcgacat cagtttaaac
     8161 gtacgttcgg cggtagtttt gatattgact ttgtccgata aaggcgtcat cagcgaaaca
     8221 ttcacgccga taacgtactt acactcattc cgaataacag aaaccggaaa atttttcaat
     8281 aatccaccgt caacataggg cacaccacca ataaccttgg gagtgaaaac gatggggacc
     8341 gaacaggatg ccaccacggc atctaccaac gatctgcctt ccgaaaaaac tacagtccgg
     8401 gccttttccc aatcggttac aactgcttta aaaggaattc tcagttgttc gaatgagctc
     8461 gaccgcaaat tgtctttcag aaatttcccg acacctgaag ttttaaaaaa acccgtcttg
     8521 ggaatggaaa atccgataaa ttcatggaat tctttctttc ggaagagctc gctaatctcg
     8581 tcgggagaaa aaccatcggc ataaaaaact ccggctaacg caccggcact ggtacctgca
     8641 attacatcgg gtttcaggcc ataactctca aataccttga aagcgcctaa atgagcaaac
     8701 ccttttgcac cgccaccgct tagcgcataa cccaaataat attgaaaatt tttcccaaat
     8761 attgccacga cggttccctt aatggtacta caaatatgct aaaaaatttg atgattgtca
     8821 aaaataaaac cgttatcaaa tggtttattt atgttttttt atacaaataa accatggtta
     8881 atgcgacaaa aatcaatacc gcaataccaa gtggcttgtt catcttcgtt cgattaaaat
     8941 tatttatttt tcagggatga ttttattata aaaccccgac gggcatttca aaggaagaca
     9001 acagcgtgcc gaacgcttta tacgctgtgc ccatcccttc cattgggatg cctatttccc
     9061 aatcatacct gatgagttct ttccaattgt ccggcatgga ggtctcgagg cccaaattca
     9121 aattctgatt taaccggtgc agggccaaat catttatcga catgcgaaaa cggtatgaga
     9181 cggtttttgc cgattccgca tccagcgttg gctgcccttt ttcatcaacc ccgtatgaaa
     9241 aggtcataaa gcaattaccc tcctctgtgt caatcgtgta agttccttta ccccgtactt
     9301 tcaacacgta atcggttgac gatgtgctct ccctgttgac aaggtagaga tcccg
//
LOCUS       DAOSDR010000066         9285 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrimonas sp. isolate AMR_MDS_1826
            NODE_2765_length_9285_cov_3.442795, whole genome shotgun sequence.
ACCESSION   DAOSDR010000066 DAOSDR010000000
VERSION     DAOSDR010000066.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863355
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrimonas sp. (wastewater metagenome)
  ORGANISM  Petrimonas sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Dysgonomonadaceae; Petrimonas.
REFERENCE   1  (bases 1 to 9285)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9285)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:10:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,720
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 35
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9285
                     /organism="Petrimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2765_length_9285_cov_3.442795"
                     /isolate="AMR_MDS_1826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2023866"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..342
                     /locus_tag="PLI30_04000"
     CDS             <1..342
                     /locus_tag="PLI30_04000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI78751.1"
                     /translation="LQFLEFGELGEVLAEVGFDGADLAVRPGGQVSPENVETELPRAF
                     KALKKAGVAIPMMVTSIDNADDPVAEKILKSASKLGITHYRMGWIAYDEKKPITENLE
                     NFKVAFAKLEK"
     gene            358..819
                     /locus_tag="PLI30_04005"
     CDS             358..819
                     /locus_tag="PLI30_04005"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HOI78752.1"
                     /translation="MRGEYQNHAGRRLGGPVWDLHPLLQSLDPEFIGVQYDVRHATVE
                     GGNSWLLGMRLVAAWIGTTDIKDFIWEKDARGAWRARTVPLGEGMVDWKTYFTEYQKL
                     NVAAPVSIHYEYDLGGAESGSKTPKMPLPEIKKWLKKDLLFLKTQINQYQL"
     gene            821..2221
                     /locus_tag="PLI30_04010"
     CDS             821..2221
                     /locus_tag="PLI30_04010"
                     /inference="COORDINATES: protein motif:HMM:NF024771.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
                     /protein_id="HOI78753.1"
                     /translation="MQPTNWNRRRFLQTSGLAAGALATGFPFPTARASESGASKKITI
                     ESVNSNFEREPLIRPFGFKGGFMTEIWQSAAWMKSASGIEKIGLCTQNVLWSDAAVFS
                     ANSESGGNALMYAMTEYALRLLKGRSFENPVSLLEEILEEVYAYGVKISGNSRLRKTF
                     ALNALVGVDNAAWLLYAAENGFSTFDEMIPAAFRPALSHRHTTVASIPLMAYNIPVTE
                     IVQAVRDGYFFMKIKIGQPGTQEEMLQKDMARISEIHKAIGDFRTPHTQSGKLPYYFD
                     ANGRYEKKETLLRLIDHTRKIGAFEQIAIIEEPFPEEVETDVSDIPVRLASDESAHTD
                     ADALTRIQMGYRAIALKAIAKTLSMTLKIAHVAKQKGVPCFCADLTVNPTLVDWNKNV
                     AARLDPFPGLGTGLLETNGHQNYRNWKTMESYHPFSDAAWRQTVDGVFNLDSDYYRKS
                     GGILAESSHYQAMFLK"
     gene            complement(2347..3969)
                     /locus_tag="PLI30_04015"
     CDS             complement(2347..3969)
                     /locus_tag="PLI30_04015"
                     /inference="COORDINATES: protein motif:HMM:NF019592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HOI78754.1"
                     /translation="MKKSNIIRYATAGIIAFALNFSFSSCDDEVLDLLPANSVAYTDA
                     FSTPKLCELSMVGCYDAAQSGYYPGNDQRRGYPFGAASIQQGDMRGEDMLNVQAFFAV
                     TYGSTYGSSSPNGQAMWECTYQMINKINICIEGFKLAASKNVITAEKAKEYEGEARFL
                     RALGFHELLLHFAYPYAHTPDASHFGIPVSTKPINSIATVEEAKSAGRATVAQTYKQI
                     LEDLDFAEANLPATRTGGLKISRATSGAAIGLKTRIYQHMGQWDKVMTESQKIVDATF
                     KSPIGSYALTAEPSGPFTNNSGNSESMFSIENSELDNCTVNGSLSQFYTYVRSLVCLS
                     PILYNADFWLATDKRRTQLVVYNTSYTARHAYWVDKYKTYAKMADWTPILRYAEILLN
                     YAEAEARTNGVTQKAVDLLNQVRNRAVTETANRFTLASFATTKDLMTAILNERRIEFA
                     GEGRRWADIHRLAKDPNYFVKTLNGAPGVPAKVNTTNVAANSWNAASGVVPPSIITIA
                     AYGYDDRRFLFPIPDSETNTNEKLKSQQNPGW"
     gene            complement(3990..7073)
                     /locus_tag="PLI30_04020"
     CDS             complement(3990..7073)
                     /locus_tag="PLI30_04020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019540973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOI78755.1"
                     /translation="MKKVALLFAFFAIGLQVLLAQTKEISGTVTSADDGSAIPGVSVL
                     VKGSTLGTVTNMDGKFILKVPQDAKSLLFSFVGMLSQEVAIGNQTIFNIQLKSEMIGV
                     DEVIVVGYGVQKKKDVTSSISQVKGADISEKATPSFVQQLAGRAAGVQITQSSGDLGT
                     PPNIRIRGVNTISSGSQPLVVVNGVPVTSGNIGGSYTNNNPLSDINPADIESIEILKD
                     GAATAIYGSRASNGVILVTTKKGKAQQTKVTYDSWFAVSQASKLYDLLDAEQFVTIAN
                     EKKKNAGSTSLPAAMDDKGTNTNWNDYVFRNAFQHSHSLSVAGGADDTQFYISGGYSK
                     QEGIVINNDFQRYTFNASVDQKLINWLKGGFSLNASYQKNTGPIKGTNSISDNMYATT
                     RMLPNVAVYNKEHPTGYNIDATSPKSLGRGANQIPIDLTVPNIVWVLKNNTQENESYR
                     LMPTGYLELNPLAGLTLKTVIGADVSLLDNVYAWLPDTGDGYGYNGLISETNYTRKRW
                     TLQNIASYVKSINEKHNIDLTGVTEWSKYEYRSVNAGARDMSSSFFMPYIISNTYNTQ
                     SSGGDYTYNGIASYIFRANYNYNSTYYIGGSVRRDGLSRLPENNRWGTFYGGSFAVRL
                     SQMGFWSSIKDVISDFRIRGSYAQVGNDDIGNFTYLDTFTAQLYGSQTGISYYQTGNA
                     DLRWEKQNIYDFGFDMSLFNRISFTAAYWSKKNSDIVLSAPTPPSLGIPWNVISQNVG
                     AVENHGLEFELGGSVYKSKQFSYNASLNFSTQNNKVTELLSDMTYEHYIIRKGESMRS
                     LYGYQYQGVNMANGYPLYKKADGSIVQGNPTDSKYYLYDASNPTKMGTASSLTASDKV
                     VLGNTIPKWFGGFDNTLKYKDFDMNIFFRFSGGNKIANVTRRDMMTMYFQNNSTEILD
                     RWQSPDKPGNGKVPIIISDKGSFLNLEADGSSRWLEDGDFLKLQNLSLGYTLPKNIAD
                     KLTFNQVRVYVQAQNLFTITNYSGLDPEVYTTTLGVDWNGNPQQRSFTFGLNVVF"
     gene            complement(7747..8601)
                     /locus_tag="PLI30_04025"
     CDS             complement(7747..8601)
                     /locus_tag="PLI30_04025"
                     /inference="COORDINATES: protein motif:HMM:NF012692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HOI78756.1"
                     /translation="MDKKMKIIGVDVGGTKISAGLVHEEKIKRQAMLPTPFDRPQLEV
                     VSSVIQAIEEVFDPSVSGIGIGVPGLVDLDEKQVLDVTNIPSWDSVPLAELVSDHFNK
                     PVYVNNDANCFAVGEKHFGKGKAYSNFVGLTLGTGAGAGIIINNHLYSGRLSGAGEFG
                     NIYYRDKNIEAYCSGQFFKDKGLDGKTMYKRASIGDPVALRLFEELGGHIGRAIATIL
                     FALAPEAIILGGSVSQSYRFFGEAMNEFLEDNFPFRRLYNQLAIEISDAENMAIFGAS
                     SLVLDAID"
     gene            complement(8737..>9285)
                     /locus_tag="PLI30_04030"
     CDS             complement(8737..>9285)
                     /locus_tag="PLI30_04030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI78757.1"
                     /translation="GSQPDRSRDVLPILEEFRKYKPTVISLAMDPEGSGPDTHYKVLQ
                     SVAAAVAEWNKEEDLSKLRIVGYRNVWFRYNPWDVEVIVPVSMNSLATLDKSFTDCYL
                     SQVNASFPSYQLDGKFSDLTQRVWMEQHKQIQFLLGKNFFYENESPLLRATHGLIYLR
                     ELTVNQFLEEAAALGKSVEGLF"
BASE COUNT         2331 a         2060 c         2216 g         2678 t
ORIGIN      
        1 ctgcagtttc tggaatttgg cgaattggga gaagtgctgg ccgaagttgg gttcgacggc
       61 gccgacctgg ctgtgcggcc gggcggacaa gtgtctcccg aaaatgtgga aaccgaattg
      121 ccacgcgcct ttaaggcgct gaaaaaagcg ggggttgcca tcccgatgat ggttacctca
      181 atcgataacg ccgacgaccc ggtggctgaa aagattttga aaagcgcttc aaaattgggg
      241 attacccatt accggatggg atggatcgcc tacgatgaaa agaagccgat taccgaaaac
      301 ctggaaaatt tcaaagtcgc tttcgccaaa ctggaaaagt aaaaccgcaa ataccacatc
      361 cggggcgaat atcaaaacca tgcgggcagg cggctgggag ggccggtttg ggatctgcat
      421 cccctgctcc agtcgctcga tcctgaattt atcggggtcc agtacgacgt gcgccatgct
      481 accgttgaag ggggtaactc gtggttgctg ggaatgcgcc tggtggctgc ctggatcggc
      541 actaccgata tcaaggattt catttgggaa aaggatgcca gaggcgcctg gcgggccaga
      601 acggttccgc tgggagaagg gatggtcgat tggaaaacct attttacgga gtaccagaaa
      661 ctgaacgttg cagctccggt ttcgattcat tatgaatatg atctgggtgg cgcggagtca
      721 ggcagcaaaa cacccaaaat gccacttccg gagatcaaaa agtggctgaa aaaagatctg
      781 ttgttcctga aaacgcagat taaccaatac cagctttgat atgcaaccga ccaactggaa
      841 tcgtcgccgg tttttgcaga cctccggtct ggctgccggc gcgctggcaa cggggttccc
      901 ctttccgaca gcaagggcgt cggagagcgg cgcttccaaa aaaataacca ttgaaagtgt
      961 caattcaaac tttgaacggg agccgcttat tcgtccgttt gggtttaagg gcggttttat
     1021 gaccgaaatc tggcagagtg cagcctggat gaagagcgct tccggcatag aaaagattgg
     1081 cctgtgtacc cagaatgtgc tctggtcgga tgctgcggtt tttagcgcca attccgagag
     1141 cggcggaaac gccctgatgt atgccatgac cgaatatgcc ctgcgcctgt tgaaaggacg
     1201 ctcgtttgag aatccggtct ctttgctgga ggagattctc gaagaggttt acgcttatgg
     1261 agtgaagatt tcggggaata gccgcctgcg gaaaactttt gcgctgaatg ccctggtggg
     1321 ggttgacaat gccgcctggc tgctgtatgc ggctgaaaat ggtttttcga cattcgacga
     1381 gatgatccct gctgcgttcc ggccggcttt gtcgcaccgg cacaccacgg tcgccagtat
     1441 cccgctgatg gcctacaaca ttccggtgac cgaaattgtg caggcggtcc gcgacggcta
     1501 tttctttatg aagatcaaaa taggacagcc cggcacccag gaggagatgc ttcaaaaaga
     1561 tatggccagg atttctgaaa ttcacaaagc tattggcgac ttccggaccc cgcataccca
     1621 atcgggtaaa cttccttact atttcgacgc aaacggacgc tacgaaaaaa aggagacctt
     1681 gttgcgcctg attgaccaca cccggaagat cggggctttt gagcagatcg cgatcatcga
     1741 ggagcctttc ccggaagagg tggaaaccga cgtgagcgac atcccggtgc gcctggcttc
     1801 cgacgaaagc gcgcataccg atgccgacgc gctcacccgt atccagatgg gatatcgcgc
     1861 cattgccctg aaggcaattg ccaaaacatt gagtatgacg ctgaagattg cccatgtggc
     1921 caaacaaaag ggggtcccct gtttttgtgc cgatcttacg gtaaatccca ccctggttga
     1981 ctggaacaaa aatgtggcag cccgcctcga tccgtttccc ggactgggca ccggcctgct
     2041 tgaaaccaac ggtcaccaga actaccgcaa ctggaaaacg atggaaagtt accacccttt
     2101 ttcggatgca gcctggcgtc aaaccgtgga cggagtattt aatcttgaca gtgattatta
     2161 ccgcaaaagc ggcggaatcc tggccgaatc atcgcactac caggcgatgt ttttaaagtg
     2221 aactgcttcc catcaaaaaa tagaggcgac catttttggc cgcctctttt tatgggttgt
     2281 tgtctcgtcc tgaaatagcg ttactttttg cgcaatgttt atttaggact agtcatagtt
     2341 caattgttac caaccgggat tctgctgaga ttttagtttt tcgttggtgt ttgtttcaga
     2401 atcagggatt gggaataaga atcgtctgtc gtcataacca tatgccgcaa ttgtaataat
     2461 cgaaggaggc acgacaccac tagctgcatt ccaggaattt gccgcaacat ttgtagtgtt
     2521 cactttggcg ggaactccag gagcgccgtt taaggttttg acaaagtaat ttggatcttt
     2581 ggctaatcgg tggatatcag cccagcgacg tccttctccg gcaaattcaa tgcgtctttc
     2641 attcaaaatg gcagtcatta aatcttttgt tgtcgcaaag ctggcaagcg tgaaacggtt
     2701 tgctgtttct gttacagcgc ggtttctaac ctggttaagt aaatctaccg ctttttgagt
     2761 aactccgtta gtccgggctt cagcttctgc ataattcagc aagatttcgg catacctgag
     2821 gatgggagtc cagtcagcca tttttgcata ggttttatat ttgtccaccc agtatgcatg
     2881 acgagctgta taactggtgt tataaactac cagttgagtt ctccttttgt ctgtagcaag
     2941 ccaaaaatct gcattgtaaa gaataggact taaacaaacc aaggaacgaa cataagtata
     3001 gaattgagac aaagagccat tcacggtaca attgtctaat tcagagtttt caatggagaa
     3061 catggattcc gaatttccac tgttgtttgt aaatggaccg cttggttctg ctgttaacgc
     3121 ataactacca ataggacttt taaaagtagc gtcaacaatt ttttgagatt cagtcattac
     3181 tttgtcccat tgtcccatgt gttgatagat acgggttttt aaaccgattg cagctccgga
     3241 ggtagctctt gatattttta agccgcctgt tctggttgcc ggaaggttgg cttcggcaaa
     3301 atcaaggtct tcaaggatct gcttgtaggt ttgggcgaca gtagccctgc ctgctgattt
     3361 tgcttcttca acagttgcaa tagaatttat gggtttagtg cttaccggga ttccaaagtg
     3421 cgatgcatct ggcgtgtgag catatggata cgcgaagtga agtaaaagtt cgtgaaagcc
     3481 cagagctctt aaaaagcggg cttctccttc atattctttt gccttttctg cggttattac
     3541 atttttggag gcagccagtt tgaatccttc aatacagata ttgattttat taatcatttg
     3601 ataagtgcat tcccacatgg cttgtccgtt gggcgaggag gatccgtaag tgctaccata
     3661 ggtaacagca aaaaacgcct gaacattcaa catgtcttct cccctcatgt ctccctgttg
     3721 aatgctggcg gcaccaaaag ggtaccccct tctttggtca tttcccgggt aatatccact
     3781 ttgggcagcg tcgtaacagc caaccatcga gagttcgcat aatttgggcg tactaaaagc
     3841 atcggtatat gctactgaat ttgccggcag cagatctagc acctcatcgt cgcacgatga
     3901 gaaagagaag tttaatgcaa atgctattat tccggctgta gcgtatctaa ttatattact
     3961 ttttttcata gtgttattct tttaaacggt taaaaaacaa cgttaagtcc aaaggtgaag
     4021 cttctttgct gtgggttgcc attccagtca acgcctaaag tggttgtgta aacctcggga
     4081 tccaatccag aataattggt aatcgtgaat aaattttgag cctggacata aacccgcacc
     4141 tgattaaaag ttaatttgtc agcaatgttc tttgggagtg tatatcccaa agaaaggttc
     4201 tgcaatttca gaaaatctcc atcttcaagc catcttgatg atccgtcggc ctccaggttc
     4261 aggaatgagc ctttatcact gatgatgatg gggactttac cgttacctgg tttgtcagga
     4321 ctttgccagc gatcaagtat ctctgtgctg ttgttttgga aatacatggt catcatgtca
     4381 cggcgggtga cattggcaat tttattccct ccggagaagc ggaagaaaat gttcatgtca
     4441 aagtctttgt acttaagtgt attgtcaaaa cctccaaacc attttgggat agtatttcca
     4501 agaacaactt tgtcgctggc agtcagactg cttgctgttc ccatttttgt ggggttactg
     4561 gcatcataca ggtagtattt gctgtcggta ggatttcctt gaacaattga tccgtcggct
     4621 tttttgtaga gcggatatcc attggccatg ttgacaccct gatattgata gccataaaga
     4681 gatcgcatgg attcaccttt gcggataata taatgttcat aggtcatgtc gctaagcaac
     4741 tcggttactt tgttgttttg ggttgagaaa ttcaaagaag cattatagct aaactgtttg
     4801 ctcttgtata cggagccgcc taattcgaac tctaaaccgt gattctcaac tgcacccaca
     4861 ttctggctaa tcacattcca gggaatacct aatgacggag gggtaggagc gctcagtaca
     4921 atatctgagt ttttcttgga ccagtaggcc gcagtaaagc tgatccgatt gaaaagagac
     4981 atgtcaaaac caaaatcata gatattttgc ttttcccatc ttaggtctgc gtttcctgtt
     5041 tgataatagg agatgcctgt ctgtgaacca taaagttgtg cggtaaaggt gtcgagatag
     5101 gtgaagtttc caatatcatc atttccaacc tgggcataac ttcctcttat cctgaaatca
     5161 ctgatcacat ctttaatgct gctccagaaa cccatttggg ataagcggac agcaaaagat
     5221 ccaccataga aggtacccca acgattattc tcaggtaatc tggaaagtcc gtctcgccgc
     5281 actgagccac caatgtaata ggtgctgttg taattgtaat tggcgcggaa aatataggaa
     5341 gcgataccgt tgtaagtata atctcctcct gatgattgcg tgttgtatgt gttcgaaata
     5401 atgtaaggca taaagaagga gctcgacata tcccgtgcac cagcattcac cgaacgatat
     5461 tcatattttg accattcagt tacgccggtt aaatcgatgt tgtgtttttc attgatgctt
     5521 ttgacgtacg aagcgatgtt ttgtaatgtc cagcgtttac gggtgtagtt ggtttcgcta
     5581 atcaatccat tatatccata tccgtctcca gtatcgggaa gccaggcata aacgttatct
     5641 aataacgaaa catcagcgcc aattaccgtc tttaatgtta aaccggcaag gggattgagt
     5701 tcaagatatc cggttggcat taagcggtag gactcgtttt cctgcgtatt gttttttaaa
     5761 acccaaacaa tgtttggaac tgttaagtca atcggaattt ggtttgcacc acgacctaag
     5821 cttttaggac ttgtagcgtc aatgttgtaa ccagtcggat gttctttgtt ataaacagct
     5881 acgttgggca gcatgcgggt agtcgcatac atgttatcag aaatactatt ggtcccttta
     5941 attggtccgg tgtttttttg gtatgaagca tttagggaga atccgccttt tagccaattt
     6001 attaattttt gatctacact ggcattaaaa gtatatcgtt gaaaatcatt gttgataacg
     6061 ataccttctt gtttgctgta tcctcctgaa atataaaatt gtgtgtcatc tgcacctccg
     6121 gccacggaca aagaatggct gtgctggaag gcattccgga atacataatc attccagttt
     6181 gtatttgttc ccttgtcatc cattgctgct ggcaatgagg tagaaccagc atttttcttt
     6241 ttttcattgg cgatcgttac aaactgttcg gcatccagta agtcatacag ttttgaagct
     6301 tgggatacgg caaaccagga atcataggtg acttttgttt gttgtgcctt tccttttttg
     6361 gtagtaacaa gtattacacc attagaggca cgagaaccat aaatagctgt tgccgcccca
     6421 tctttcaaaa tttcgattga ttcgatgtct gcaggattaa tgtccgacaa gggattgttg
     6481 ttggtatagc tacctccgat gtttcctgaa gttacgggga ctccgtttac aacaactaat
     6541 ggctgagatc ctgaggaaat ggtgttaact ccacgaattc ggatatttgg aggggtaccc
     6601 aaatctcctg aactttgtgt gatttgaacg ccggcagcac gtcctgccaa ctgttggaca
     6661 aagctcggag tagctttttc cgaaatgtcg gctcccttca cctgagatat tgaactggtg
     6721 acgtctttct ttttctgaac accatatcca actacaatca cttcgtccac cccaatcatt
     6781 tctgatttca actggatgtt gaagatggtc tggtttccga ttgcaacttc ctgcgagagc
     6841 atgcctacaa aagaaaataa gaggcttttc gcatcttgcg gaacctttaa aatgaattta
     6901 ccatccatgt tggtgacggt acccagtgta gatcctttga ccaagaccga cacgcccggg
     6961 attgcacttc catcatctgc ggaggttacg gttcctgaga tctctttggt ttgagccagc
     7021 aggacttgta atccgattgc gaaaaatgca aacagtaatg caactttttt catagaaatg
     7081 attttaagtt agtaaataaa cagcaaacaa tattttgtct ccaagcatca agtcctgccc
     7141 aatgtttttc ccctcttgtt cttttcgggt caggactttt aagcattttt aacaaatata
     7201 aaaacaatgg gatatatata atatcttatt gttaataaag gtaaattttt attgacccct
     7261 ttttttttaa taagattttg ttttttaagt ggctgtttgt gagtgttttg gttgcttgtt
     7321 gttcggattt tttttaaagc ttttgaacct tgaggatttt tttaggttat ttttttggtt
     7381 tttttaccgc gaattttaaa tgaatttcga gacaattcgg tcgtttttgt gacaaaaaaa
     7441 agatttttgt taccgtgagg gatttttttg aagccttcgg agaagggttg agtttttcta
     7501 aattatcata attgcaagtc aaaatttttt tctgccgata tgctgaaaaa ataagcggta
     7561 ctgggcagtt tccatttgtt tcgattgaaa agaaaacgta ttttcgtgcc ctctgaacga
     7621 acaatcagtt cggaattacc atttgtgagc cgctcttccg caagggaggg cggctccttg
     7681 tttttgcggg gttggtccaa aaaaaaggcc ccgaaaccgg agccttatta tatcgttgcc
     7741 tgttttttag tcgatcgcgt cgagcaccag cgaagaggcc ccaaaaatgg ccatgttttc
     7801 ggcatcggat atttcgatgg cgagctggtt gtacaacctt ctgaacggga agttgtcttc
     7861 caggaattcg ttcatcgctt ctccgaaaaa ccggtacgat tgactgacgg acccgcccag
     7921 gatgatagct tcgggggcca gggcaaacag gatcgtggca attgcccgtc caatgtgccc
     7981 gcccagctct tcaaacagcc gcagggctac ggggtcacca atcgaggccc gtttatacat
     8041 cgtttttccg tccagtcctt tgtctttaaa aaattgtccg ctgcagtatg cttcaatgtt
     8101 tttgtcgcgg tagtaaatat tgccgaattc acctgctccg ctcagtcttc ccgagtaaag
     8161 gtggttgttg atgatgatgc ctgctccggc gccggtcccg agggtgagcc cgacgaaatt
     8221 ggaataggcc ttcccttttc cgaaatgttt ttcgccaaca gcaaagcagt ttgcatcgtt
     8281 atttacataa accggcttgt tgaaatgatc ggaaaccaac tcggccagcg gaacagagtc
     8341 ccagcttggg atattggtca catcgagaac ctgtttctcg tccaggtcaa ccagtcccgg
     8401 taccccgatc ccgatgcccg aaacggaagg gtcaaacact tcttcaatag cctggatcac
     8461 cgaacttacc acttcgagtt gaggacgatc gaacggagtt ggcaacattg cctgccgttt
     8521 gatcttctct tcatgcacca gtcctgcact gatttttgtc ccccctacgt caacaccaat
     8581 gattttcatt tttttatcca ttcagttttg aatcatacct gccagtggga agcacccagg
     8641 cgcttcccgt tttggcggta caaatataat ttcttttgtt gtttcaatat gcaaacagtc
     8701 agttgcttat gtgcttctgg tttgtctttt cgatggtcag aaaaggcctt cgactgattt
     8761 ccccagggcg gctgcctctt caagaaactg gtttactgtc agttcacgta ggtaaatcag
     8821 tccgtgggta gctcgcagca acggagattc attttcgtaa aagaagtttt tccccagcaa
     8881 aaactgaatc tgcttgtgct gttccatcca cacccgctgg gtgaggtcgc tgaacttccc
     8941 gtcgagctgg tagctgggga atgaggcgtt gacctggctc aggtaacaat cggtgaacga
     9001 cttgtcgagg gtagccagcg agttcatgga taccggtacg atcacttcca catcccacgg
     9061 gttgtaacgg aaccagacgt tccggtagcc aacgatccgg agtttggaga ggtcttcttc
     9121 cttgttccat tcggccacgg cagctgcaac agactgcaaa actttgtagt gggtgtcggg
     9181 cccgcttcct tccgggtcca tggccaggct gatgacggtg ggtttgtatt tccggaactc
     9241 ttcaagaatg ggcaatacgt cgcgcgagcg gtcgggctgg ctgcc
//
LOCUS       DAOSDR010000067         9283 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrimonas sp. isolate AMR_MDS_1826
            NODE_2768_length_9283_cov_4.264304, whole genome shotgun sequence.
ACCESSION   DAOSDR010000067 DAOSDR010000000
VERSION     DAOSDR010000067.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863355
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrimonas sp. (wastewater metagenome)
  ORGANISM  Petrimonas sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Dysgonomonadaceae; Petrimonas.
REFERENCE   1  (bases 1 to 9283)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9283)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:10:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,720
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 35
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9283
                     /organism="Petrimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2768_length_9283_cov_4.264304"
                     /isolate="AMR_MDS_1826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2023866"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..241)
                     /locus_tag="PLI30_04035"
     CDS             complement(<1..241)
                     /locus_tag="PLI30_04035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019538381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HOI78758.1"
                     /translation="MEPDNLAETLLAGRFPFERAATFCVYSRGGVLQPLIHQLKYNNK
                     KEIGVLLGKLFGKDLIGSEFILPIDLIVPVPLHPQK"
     gene            complement(383..868)
                     /locus_tag="PLI30_04040"
     CDS             complement(383..868)
                     /locus_tag="PLI30_04040"
                     /inference="COORDINATES: protein motif:HMM:NF014668.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory protein RecX"
                     /protein_id="HOI78759.1"
                     /translation="MERVERKPVSEKTAYQRMARVCSMKECCSFDIRQKLLRMQLADN
                     AVNNIMSLLLKQKYIDDSRYTRSFISDKLRFSKWGKTKIVFTLRQKQISQETIDQALE
                     AFSDEVLLQSLKPLLEKKLKSVKGKSGYEKRTKVIRFALGRGFSMPDILECLGKMPEE
                     N"
     gene            complement(872..1711)
                     /gene="prmC"
                     /locus_tag="PLI30_04045"
     CDS             complement(872..1711)
                     /gene="prmC"
                     /locus_tag="PLI30_04045"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HOI78760.1"
                     /translation="MQDVKNFLKNELKTHFSPEEISSLTRLILEKEFSIPLADILTCK
                     FNHLSDAAVKKLSEIIDKLKNAEPIQYILGETDFFGLPFYLDSSVLIPRPETEELVQW
                     VITSTNNASAKILDIGTGSGCIAVTLAKKLPHAEVHAWDISPDALEVARKNAERNGVH
                     LFFLEKDILQESVSDKMFDIIVSNPPYVTESEKSDMEDNVLRFEPHLALFVTDENALV
                     FYEKIADFARIYLNKGGALFFEINREKGCQVEALLQQRGFKNIERVKDISGNERMIKA
                     VKE"
     gene            1746..2807
                     /gene="ribD"
                     /locus_tag="PLI30_04050"
     CDS             1746..2807
                     /gene="ribD"
                     /locus_tag="PLI30_04050"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019538378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HOI78761.1"
                     /translation="MTTDEKFMYRCLQLAQKGEGFARPNPMVGAVIVHNGQIIGEGYH
                     RQFAEAHAEVNAVLSVKDQALLSSSTLYVSLEPCAHHGKTPPCAELIIEKKIPKVVIA
                     VSDPNPKVSGKGIEKIQKSGIEVTTGILQKEAEELNRAFFVNQLYNRPYVILKWAQSS
                     DGFIDYRRSTAEEAPAQISNDLTHSIVHRFRTHVQGIMVGTDTALLDNPKLTARKWFG
                     NNPTRIVIDREAKIPSNAAIFSEDSAVIVFTQSGYPVRKRNIKPIIIDFNRDTNRQIL
                     KHLYAEKIYSVLVEGGTRLLSTFIDKNLWDEAYIEIAATKLISGVKAPEISMDNAITK
                     KYLDSTQHHLKNKITQNFL"
     gene            2892..4247
                     /locus_tag="PLI30_04055"
     CDS             2892..4247
                     /locus_tag="PLI30_04055"
                     /inference="COORDINATES: protein motif:HMM:NF039698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6242 domain-containing protein"
                     /protein_id="HOI78762.1"
                     /translation="MTLKYLHQTASILLWVLVFSSCLNSSQSDIELSHDAQIYSFSMS
                     SKKDTTSALSGTRFTIDQINNKIFNRDSLPYLFHVDSIYLNIAGKSSYTLPRIVLNLQ
                     DKDSSYLWNGKDSVAFKRLKSIETTAEDGKTVKLYEFKANIHQQDPYILNWAKITQNQ
                     LINPVEQQKTILHGGKFITYYKSGAMIKASSSLSSDGKNWTPVTVSGLPVTVKTNTIL
                     STTNNSGSTAYALNTDNNIYTSTDGLVWSKVTSDYPVIAIYGKLPSASGEFAILTAVN
                     DAGTLKFALTKDFTTFTVKSALPSDNTLPTVDFSAVSLENPTVFSAKYIILSGGKDKN
                     NIVNNKLWIIQELNGDITHLSEVSSISLQLSRLFLYDNKVYLMTYETGKNKLYYSENY
                     GLNWISGGTNQTLPDNFTGRMHASVITDTNNFIWILGGESGAQVPIVDVWRGRLNKLA
                     E"
     gene            4297..4992
                     /locus_tag="PLI30_04060"
     CDS             4297..4992
                     /locus_tag="PLI30_04060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006843846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6089 family protein"
                     /protein_id="HOI78763.1"
                     /translation="MKKSEFVFFLLLIAAVFDTDEVKAQEYKHESGGMLGISFYLGDA
                     NKTRLYLHPGFTGGILYRYNINFHWAAKANLLAGKVSGNTRDANTVFPLAQQTSFNRT
                     FIDWGGQIEFNFLPFSDKFTYKDAKPYTPYVFAGAGVTFATGSKPFFNTNVPVGIGFK
                     YKIKERLNIGLEFSVRKLFGDGFDAAEKNADWGLDAPYGIKSSIFKNQDWYSLTMIFL
                     TWDFGIRHDPCCE"
     gene            5017..5769
                     /locus_tag="PLI30_04065"
     CDS             5017..5769
                     /locus_tag="PLI30_04065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006800924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HOI78764.1"
                     /translation="MSLLDKIDKENIPQHVAIIMDGNGRWAKARGLDRTEGHKEGALS
                     VQKVVKAAAKAKVKFLTLYAFSTENWLRPEEEIHTLMELMVYTIANETDNLVKNGIRL
                     QCIGNIQRLPENTRKALNRCIEQTSSGEDLTLVLALSYSSRWELANAAKAIASSVKNG
                     ELDESSITEETINEYLATKGMPELDLLIRTGGDIRISNFLLWQAAYSELYFTGVFWPD
                     FDEECFYEAILDFQKKERRFGKTSEQLEKENQ"
     gene            5828..8527
                     /locus_tag="PLI30_04070"
     CDS             5828..8527
                     /locus_tag="PLI30_04070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009121866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="POTRA domain-containing protein"
                     /protein_id="HOI78765.1"
                     /translation="MLKKTFHLVILLILFSQVRSFGQITTNDTVKTAPAEDVPVVNYT
                     ATPKKYIIAGIDVTGIEGTMYEAQPFVLVSFSGLAKGQEIQIPGEEITNAIQRFWKQG
                     LFSDIKILQTKIEGDSTWLEIKLTDRPRVSDIRYIGMKKSERDDIEKKISVVKGNQIT
                     PNQINSAKTIIEKYFDEKGFGEAVISIMQRPDASAKNQVILDIEVDKKEKIKVNRINI
                     EGNESISDRTLKWAMKKTNEKGNILHLFRTKKFVEDLYKEDKKNFINKYHEFGYRDAE
                     IIRDSVYKNDEKTVNIDIVVEEGPKYHVRSINWVGNTQYNSIDLGRMLNMNPGDVYNQ
                     KKLQERLTTDDDAAINLYQNNGYLFSGIDPIEINIENDSVDLELRVTEGPKATIKKVI
                     IQGNDRLYEDVIRRELRTKPGAVFSKEDLIRSVREIAQTGHFDPENLQPGVNPNPEDG
                     TVDLTYPLTSKGNDQIEFSAGWGVTGLVGKLSLKLNNFSLKNLINRENYRGIIPQGEG
                     QTLILSAQTNGRYYQSYQFSFIEPWFGGKRPNNFSLSAYYSRYTGLNSDYYTQMNPYM
                     YGGGYGGYGGYSPYGYGGYSPYGYGGYGGYGGYGGYGYQDMMEYSYDPNQVFEMIGAS
                     IGYGKRLEWPDDYFQVMAELGYQRYNLKNWSWQQFPFQTGSSNSVTFGITLQRNSIDN
                     PIYTRLGSQFTLSVSATPPFSLFNNTDYSKMAYSDPAKYTWNEYHKWKLKIRTYTPLA
                     PLTVKRTPVVATRAEFGILGHYNKNKLTPFETFDVGGDGMSGYSSSFATENIALRGYD
                     NNSIAQQGRAYTRLGLELRYPLILEPNSTIYAVGFLEAGNAWHNLKDFNPFDLKRSAG
                     VGARIFLPMIGLMGIDWAYGFDPIRGSRSNSGSQFHFIIGQEF"
     gene            8615..9127
                     /locus_tag="PLI30_04075"
     CDS             8615..9127
                     /locus_tag="PLI30_04075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019538373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HOI78766.1"
                     /translation="MKKTLFLVGFFLALTIGSTYAQKFAFIDMEYILGKIPAYENGNK
                     QLENVSKQWQSEVDKAAQEVEAMYKKYQADLVFLAGEEKTKRENEIVAKENEINTLRN
                     KYFGQQGELFKRREAIMKPIQDDIYNAVKEIAAVNSYQAVVDRASATSIIFASPDIDI
                     SDQVLSRLGY"
BASE COUNT         2893 a         2050 c         1949 g         2391 t
ORIGIN      
        1 gtttctgggg atgcagcgga acagggacta ttaaatcgat gggtagaata aattcactac
       61 ctatcaaatc cttgccaaac agttttccca acaacactcc tatttctttt ttgttgttgt
      121 acttgagttg gtgaataagc ggctgtaaaa cacctcctct tgaatatacg cagaaagtgg
      181 cagcccgctc aaacgggaat cttcctgcca aaagagtttc agcaagatta tccggctcca
      241 taaaattgtt cgttttggga agggcatgta agcattgtaa acaaacgccc tgctcggaag
      301 gcaaaagtcg aatactacaa accgggcagc attccggaaa aaagagattc gataattcgt
      361 ttaacaggct tttcacactc attcaattct cctccggcat ttttcccagg cattccagaa
      421 tatccggcat cgaaaatcct ctgcccagcg cgaaacgaat aaccttagtc cgcttttcat
      481 atccggactt tccttttact gatttcagct tcttctccaa caaaggtttt aacgattgaa
      541 ggagaacctc atcagaaaac gcctccaatg cctggtcaat agtttcctgc gaaatttgtt
      601 tctgtcgcag ggtaaaaaca attttagttt ttccccactt gctaaaacgg agtttatcgc
      661 tgatgaagct gcgagtatat cgggaatcgt cgatgtattt ctgtttcaac aaaagagaca
      721 taatgttgtt gactgcatta tcagcaagtt gcatccgtag caatttctgc cggatgtcaa
      781 acgaacaaca ctccttcatg gaacagaccc tcgccatgcg ctggtaagct gttttttcgg
      841 aaactggttt tcgttctact cgttccatac cctactcttt taccgctttt atcatccttt
      901 cgttacccga aatatccttt acccgttcga tatttttgaa tcctctttgc tggagcaatg
      961 cttcaacctg gcatcccttt tcccgattga tctcgaaaaa aagggcccct cccttgttca
     1021 ggtatatccg ggcaaagtcc gctatctttt cgtagaaaac caacgcattt tcgtccgtaa
     1081 cgaacagagc caggtgaggc tcaaacctca agacattgtc ctccatgtcc gatttttcag
     1141 attcagtcac ataagggggg ttgctcacaa taatgtcaaa catcttatcc gaaacagatt
     1201 cctgcaaaat atctttttcc aggaaaaaca aatgtactcc gtttctttcc gcattttttc
     1261 gggccacttc cagcgcatct ggcgaaatat cccacgcatg tacttctgca tgaggcagtt
     1321 tttttgccaa cgtaacggca atacagccgc ttccggttcc aatgtcaaga atttttgcgg
     1381 atgcattgtt tgtcgaagtt atcacccact gtaccaattc ttccgtttcg ggacggggaa
     1441 ttaaaacaga actatcgagg taaaagggta atccaaaaaa atcggtttca cccaaaatgt
     1501 attgaatggg ttcagcattc tttaatttat cgataatttc gctcagtttc ttcaccgctg
     1561 catcggataa atggttaaat ttgcatgtta aaatatctgc cagtgggatc gaaaactctt
     1621 tttccagaat cagccgggtt aagctgctga tctcctccgg cgaaaagtga gtttttaatt
     1681 cattttttag gaagtttttc acatcttgca ttgttggatc gttttactaa acaaagatag
     1741 aaaaaatgac gacagacgaa aaatttatgt accgatgcct gcagctcgct caaaagggtg
     1801 aaggttttgc aagacccaat cccatggtgg gagcagtcat tgtgcataac ggacaaatta
     1861 tcggtgaagg ttatcaccgg cagtttgccg aggcgcatgc cgaagtgaat gccgtactct
     1921 ccgtgaaaga tcaggcatta ctttcttctt ctaccttgta cgtctcactc gaaccttgcg
     1981 ctcaccatgg aaaaactccc ccttgtgctg agttgatcat tgaaaaaaaa attccgaaag
     2041 tggtcatcgc cgtttcagac ccgaatccta aagtgtcggg aaagggtatt gagaagatac
     2101 aaaaatctgg aattgaagta actacaggta ttctccaaaa agaagccgaa gaattaaacc
     2161 gggccttttt tgtgaaccaa ttatataacc gtccctatgt gattcttaaa tgggcacaga
     2221 gttccgacgg atttatcgat taccgacggt caacagcaga agaagcaccg gcacagatat
     2281 ctaatgatct gactcatagc attgtccata ggttccgcac tcacgtgcag ggaataatgg
     2341 tgggaaccga cacagcctta ttggataacc caaagctgac agcccggaag tggttcggta
     2401 acaatcctac ccggattgtt attgacaggg aagccaaaat cccttcaaat gcagcaatct
     2461 tcagcgaaga ctcggccgtt atcgttttta cccaatcagg ctatccagta agaaaaagaa
     2521 acatcaagcc catcatcatt gatttcaaca gagataccaa caggcagatt ctaaaacatt
     2581 tatatgcgga aaaaatctat tcagtcctcg ttgaaggtgg cacccgcctc ttatcaacct
     2641 ttatagataa aaatctttgg gacgaagcgt atattgaaat tgctgcaacg aaactaattt
     2701 caggagtgaa agcccccgaa atctctatgg ataacgctat tactaaaaag tatttagact
     2761 ctacccaaca tcatttaaag aataaaataa ctcaaaattt tctttaaata tttattttgc
     2821 tatggtttaa gtgaaaaatc tttctacttt tgcagaactt atacgtattg tacaatattt
     2881 ttttgaaaca aatgacatta aaatatctcc accagactgc ttctatacta ttatgggttc
     2941 tggtattctc ttcctgtctt aattcatcgc aaagcgatat cgagttgtct cacgatgctc
     3001 agatttattc gttcagcatg tcctccaaaa aagacacaac cagcgcactt tcggggacga
     3061 ggttcactat tgaccagatt aacaacaaga ttttcaaccg cgactctctg ccttatcttt
     3121 tccacgtaga cagcatctac ctgaatatcg ccgggaaatc aagttacacc cttcccagga
     3181 ttgtacttaa cctgcaagac aaggacagct cttacctatg gaacggaaaa gactctgtcg
     3241 cttttaaacg gttaaaatcc attgaaacaa ctgctgaaga cggtaaaacg gtgaagttat
     3301 acgagttcaa agcaaacatt caccaacagg acccctacat cttgaactgg gcaaaaatca
     3361 ctcaaaatca actaatcaat ccagtagaac aacaaaaaac cattcttcac ggcgggaaat
     3421 ttatcactta ctacaaatcg ggggcaatga tcaaggcttc ctcttcttta tcttcagacg
     3481 gaaagaactg gaccccggta actgtctccg ggctgccggt cactgtaaaa accaacacta
     3541 ttctctcaac aacaaacaac tcaggatcaa ctgcttatgc cttgaataca gataacaaca
     3601 tatatacatc gaccgatgga ttggtctgga gtaaagtaac ttccgattat ccggttatcg
     3661 ctatatacgg aaaactacct tcagcgtcgg gagaatttgc gatacttacc gctgttaatg
     3721 atgcggggac acttaaattt gctctcacaa aggatttcac aacattcacc gtgaagagtg
     3781 cactaccttc cgacaacaca ttaccaactg ttgatttctc ggcggtaagt ttagagaatc
     3841 ccacagtttt ctctgccaaa tacatcattc tctccggagg aaaagacaag aacaatatcg
     3901 tgaacaataa gctctggatt atccaggaat taaacggaga tatcacgcat ctctcagagg
     3961 tttcgtccat ttccctccag ttaagtcggt tgttcctgta cgataacaaa gtatatttga
     4021 tgacgtatga aacaggtaaa aacaagttat attattcaga gaattatggg ctaaactgga
     4081 tttcgggggg aaccaatcaa acgcttcccg acaatttcac agggagaatg cacgcatcgg
     4141 taattaccga taccaataat ttcatctgga ttctcggcgg agaatccgga gcccaggtac
     4201 ccatcgtaga tgtttggaga ggaaggctca acaagttagc ggaatagttg aaaaaccggg
     4261 aattagaagt tggataaccg gggtattaat tcggcaatga aaaaaagtga atttgtcttt
     4321 tttctgcttt tgatcgctgc agtttttgac accgacgaag ttaaagcgca ggaatacaag
     4381 cacgaatccg gcgggatgtt gggaatatct ttctatttgg gtgatgccaa caaaacaaga
     4441 ctctatctac atccgggatt tacgggaggc attttgtacc ggtacaacat taattttcat
     4501 tgggcagcaa aagcaaacct gcttgccggt aaggtatcgg gaaatacaag agatgcaaat
     4561 actgtttttc cgctggcgca acagacatcg tttaacagga catttatcga ttggggcgga
     4621 cagattgaat tcaactttct gcctttcagt gataagttca cctacaagga tgcaaagcca
     4681 tataccccgt atgtttttgc cggtgccgga gtaacttttg ccacaggtag caagccattt
     4741 ttcaatacaa atgtaccggt aggtatcggt tttaaataca agataaaaga aaggctaaac
     4801 atcgggctcg agttttccgt acggaaatta ttcggagacg gtttcgatgc ggcggagaaa
     4861 aatgctgatt ggggtttaga tgctccttac ggcattaaaa gcagtatttt taaaaaccag
     4921 gactggtatt cacttacaat gatctttctg acctgggact tcggaatcag acatgatccg
     4981 tgctgtgaat gatcaacaga gaatttaaaa aaacatatgt cattacttga caagattgat
     5041 aaggagaata ttccgcaaca cgttgccatc attatggatg gcaatggcag gtgggctaaa
     5101 gccagaggtt tggacaggac cgaaggacac aaagaaggcg ctttatccgt acaaaaagtg
     5161 gtgaaagcag ctgcgaaagc aaaggtgaag tttcttacct tgtacgcttt ttctaccgag
     5221 aactggctta gacctgaaga agaaatacat acattgatgg agttgatggt gtacaccatt
     5281 gccaacgaaa cagacaactt ggtcaaaaac ggaatccgct tacagtgcat tggaaatatt
     5341 caacggctgc cggaaaatac ccgaaaagcg ctgaatcgct gtatcgaaca aacgtcctct
     5401 ggagaagacc tgaccttggt attggccttg agttattcat cgcgatggga actggccaat
     5461 gcggcaaaag ctattgcttc cagcgtcaaa aacggagagt tggatgaaag ttccattacc
     5521 gaggagacca ttaatgaata cctggctaca aaaggtatgc ccgaacttga tttactcatc
     5581 agaaccggag gggatattcg tatcagtaac tttctgttgt ggcaggcagc ctactccgag
     5641 ttatatttta ccggcgtttt ctggcccgac ttcgatgaag aatgctttta tgaagccatc
     5701 ctcgatttcc agaagaaaga aaggcgcttt ggaaaaacca gtgaacaatt agaaaaggaa
     5761 aaccaataaa tttcgatata cagtactgaa aagctgaaca gttgaagcga aagaaaaaaa
     5821 aatcacaatg ttgaagaaaa cattccattt agttatactc ttaatcctgt tttcccaggt
     5881 gaggtcgttt ggacaaataa ccaccaacga tactgttaaa actgcacctg ctgaagatgt
     5941 tcccgtggtg aactacacgg caacacccaa aaaatacatt attgccggta ttgatgttac
     6001 cggcattgaa ggaacgatgt atgaagcaca gccttttgta ttggtcagtt tttccggatt
     6061 ggcaaaagga caggaaattc agattcccgg agaagaaatc accaatgcca tacaacggtt
     6121 ttggaaacaa ggcctgttct ccgatattaa aatcctgcaa accaaaattg agggcgacag
     6181 cacctggctc gagatcaaac tgacagacag gccacgtgta tcagatatcc ggtatatcgg
     6241 catgaaaaaa agcgaacgcg acgatatcga gaaaaagatt tccgtggtca agggaaacca
     6301 gattactccg aaccagataa acagtgcaaa aaccatcata gaaaaatact tcgatgagaa
     6361 aggatttgga gaagcagtca tctccattat gcaacgcccc gatgcaagcg caaaaaatca
     6421 ggtcattctc gatatcgaag tggataaaaa agaaaaaata aaggtcaata gaatcaacat
     6481 agagggtaat gaatccatct cggatcgcac gcttaaatgg gcgatgaaaa agacaaacga
     6541 gaaaggcaat attctacacc tgttccgtac gaagaagttt gtagaagacc tctacaaaga
     6601 agacaagaaa aacttcatca acaaatacca cgagttcggc taccgcgatg ctgaaataat
     6661 ccgcgactcc gtctataaga acgatgaaaa aacagtcaac attgatattg tggtagagga
     6721 aggccctaaa tatcacgtac gctccatcaa ctgggtggga aacacgcaat acaactccat
     6781 cgatctgggc agaatgttaa acatgaatcc aggcgatgtc tataaccaga aaaaattgca
     6841 ggaaaggctg actaccgacg atgatgcagc catcaacctg tatcaaaaca acggttacct
     6901 gttttcgggc attgacccga ttgaaataaa catagaaaac gattccgtgg atctggaact
     6961 tcgggttacc gaagggccca aggcaacgat caagaaagta atcattcagg ggaacgatcg
     7021 cctttacgaa gatgtcatcc gccgtgaatt acgtactaaa ccgggagccg ttttcagcaa
     7081 agaggacctt atccgctcgg tacgtgagat agctcagacc ggacatttcg accccgaaaa
     7141 cctgcagccg ggcgtaaatc caaatccaga agacggcact gtagacctta cctacccgtt
     7201 gacctccaaa ggaaacgacc agattgaatt ttctgccgga tggggtgtta ccggattggt
     7261 gggtaagctg agcctgaaac tcaacaattt ctcgttgaaa aacctgatta accgggagaa
     7321 ctacaggggc attatccccc agggtgaagg acaaacgctt attcttagtg cacagaccaa
     7381 cggacgttat taccagtcct accaattttc gtttatcgaa ccctggtttg gaggcaaacg
     7441 tcccaacaac ttctcgctaa gcgcctatta ttcaagatat accgggttaa actccgatta
     7501 ttatactcag atgaatccct atatgtacgg tggggggtat ggaggatacg gcggatatag
     7561 tccttacgga tatggcgggt atagcccgta cggatacgga ggatatggag gatatggcgg
     7621 ttacggcggt tacggttatc aggatatgat ggaatattca tacgacccca accaagtatt
     7681 cgaaatgatt ggcgcatcca tcggctacgg gaaaaggctg gagtggcccg atgattactt
     7741 tcaggtcatg gctgaactgg gatatcaacg ttacaacctc aaaaactggt cgtggcaaca
     7801 gttcccattc cagacaggtt caagtaacag cgtcacgttc ggtataacgt tgcaaagaaa
     7861 ctccatcgac aatcccatct atacacgcct gggatcgcag tttacattga gcgtaagcgc
     7921 aacacctccg ttctcgctct tcaacaacac cgactattcc aaaatggcat actccgatcc
     7981 cgcaaaatac acttggaacg agtaccacaa atggaaactg aaaataagga cgtacacccc
     8041 cctggctccg cttactgtaa aaagaactcc ggtagtggca acccgggcag aattcggaat
     8101 tttaggacac tacaataaaa ataaactgac ccctttcgaa acgtttgacg taggtggtga
     8161 cggaatgagc ggatactcct cttcttttgc aacggaaaac attgctttgc gcggttatga
     8221 caacaactcc atcgcacagc aggggcgcgc ttatacccgc ttggggctgg aactccgtta
     8281 cccgttaata cttgaaccta actcaaccat ttacgcagta gggttcctgg aagcagggaa
     8341 tgcatggcat aacctgaaag actttaatcc ttttgacctg aaacgctctg ccggtgttgg
     8401 cgcccgtatc ttcctgccca tgatcgggtt gatggggata gactgggcgt atggatttga
     8461 tcctatccgg ggaagccggt cgaacagcgg aagccagttt cactttatca tcggacagga
     8521 attttaattt aacaaaattt attcctgccg gtataaacga aatgcatttt ttgttgtctt
     8581 tacattcact gaacaattaa aacgactaac gaatatgaaa aagactttat ttttagttgg
     8641 cttcttctta gccctgacaa tcggaagtac ttacgcacaa aaatttgctt tcattgatat
     8701 ggagtatatt ctcgggaaaa tcccggctta cgaaaacggc aacaagcagc tcgaaaatgt
     8761 atcaaagcaa tggcagagcg aagtggataa agcagcccag gaagtagaag ccatgtacaa
     8821 aaaatatcag gcagacctgg tttttctggc cggtgaggag aaaacgaaac gcgagaacga
     8881 aatagttgcc aaggaaaacg aaatcaacac gctacgcaac aaatatttcg ggcagcaggg
     8941 tgaattgttc aaacgccgcg aagcgataat gaaacctatc caggatgata tttacaatgc
     9001 tgtaaaggag attgccgcgg tcaactctta ccaggcagtt gtcgacagag cttcggccac
     9061 atccatcata tttgcatcgc cggacattga tatcagtgat caagttttat ctcgattagg
     9121 atattaataa ccaaatattt attatctttg cgacgcaaaa acagaaactg accaaggcta
     9181 aaccgacatt gattcagtct aaacgaaaaa attaataaaa ttcaatacat taatcatgac
     9241 taaaaaatta atcatcttat tgctcgcgat tacccctatc gct
//
LOCUS       DAOSDR010000068         9246 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrimonas sp. isolate AMR_MDS_1826
            NODE_2784_length_9246_cov_4.261016, whole genome shotgun sequence.
ACCESSION   DAOSDR010000068 DAOSDR010000000
VERSION     DAOSDR010000068.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863355
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrimonas sp. (wastewater metagenome)
  ORGANISM  Petrimonas sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Dysgonomonadaceae; Petrimonas.
REFERENCE   1  (bases 1 to 9246)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9246)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:10:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,720
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 35
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9246
                     /organism="Petrimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2784_length_9246_cov_4.261016"
                     /isolate="AMR_MDS_1826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2023866"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            201..632
                     /locus_tag="PLI30_04080"
     CDS             201..632
                     /locus_tag="PLI30_04080"
                     /inference="COORDINATES: protein motif:HMM:TIGR00738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HOI78767.1"
                     /translation="MKINSKIRYGLRTMIEIACCKEPTGILQKDIAKNQNISVKYLDY
                     IISALKLKGLISNTKGRGSGYMLARPADKITMLDIYTAFEQIVIIECVKNRSFCERAT
                     HQCTANNYWKQFHHQIIEILSAKTLEQIIKETKYDCPEICE"
     gene            899..1489
                     /locus_tag="PLI30_04085"
     CDS             899..1489
                     /locus_tag="PLI30_04085"
                     /inference="COORDINATES: protein motif:HMM:NF016176.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HOI78768.1"
                     /translation="MKSNFLFKFPTVLIAFIFVLTGTTVKAQTLKIKSSTITISGTTN
                     VHPYTTTSTQASGELTVSNNKATVLNVNVPVKSIINGEKLMDKKTHETFNEPKNPFIN
                     FKMSEVKSITTNGSDISVVIGGTLAMAGTSKTVTLEANGKEIKPGTYTFEGSLPLKMS
                     DYNMKAPTAMLGVMKVGDQVTVKYNVTFEGPAVQFN"
     gene            1540..2880
                     /locus_tag="PLI30_04090"
     CDS             1540..2880
                     /locus_tag="PLI30_04090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI78769.1"
                     /translation="MKQRGLLLAATLLASGMMFAQLRPTNKDGINVFETPKTETEFEG
                     FNVQLGGALTLPFSMLDHSNTVTRESGYSYDYANPAANSLVPLTSGFGLPQANLYIKS
                     NLSDGIYLNFELYLASRHHNETWVKGGFLQFEKMEFLPWDFVDEIMKYTTIKVGQFDV
                     NYGDAHFRRSDGGLTFYNPFMENHIMDEFATEIGAEVDVHVGDFILVGAVTNGKLNND
                     LTKIDTTRAQTKYSNGVHNPAWIGKLAYDKQINSQFRLRASASGYYTAGSVRNTLFGG
                     DRTGSNYYGVMYNAPISNANFTNGRFNPGFGDKVAAVMGNLFLKFSPVQGFSLESFTT
                     LENAGGRGANEPDVRKSNQFVTDLVVRFGADEDFYVGARYNTMKADMGAAQGEPNHYE
                     VDINRVAIAAGWYMTKNVMAKIEYVNQKYNGFPARSIQDGAEFNGLTLQGSIAF"
     gene            2943..3581
                     /locus_tag="PLI30_04095"
     CDS             2943..3581
                     /locus_tag="PLI30_04095"
                     /inference="COORDINATES: protein motif:HMM:NF016176.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HOI78770.1"
                     /translation="MKALFALLLIGILTTGAVVAQTSSAVVAVDVNKNSSLSINGSSN
                     IVNFTLTQSSENFIQKNMIITASQNDKKLYLSENRLIVPVKRFTSSNKMALRDFYKLL
                     KSDEYPTMDIQLNHIDLPENPKLATGVAVVDVTITGVTKKYQFPIKTEKNGTRYIFDI
                     EKHINIRDFGLEPPVQMMGMLKVDEMININLKMICNIQPAESAQLNLSGSSE"
     gene            complement(3591..4055)
                     /locus_tag="PLI30_04100"
     CDS             complement(3591..4055)
                     /locus_tag="PLI30_04100"
                     /inference="COORDINATES: protein motif:HMM:TIGR03511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility lipoprotein GldH"
                     /protein_id="HOI78771.1"
                     /translation="MRSKLFVGFLVVFACLSSCDEGEVYYRFHPIKNSSWSKQATANF
                     LIDSLSVDPDKRYDIVLEIVNNNRYPYQNIWLLVQQNITDTTFVGDTVEITLADPQGK
                     WLGKGTAGLYQLSVPYKTSVALDSARAYLIRIRQVMKDNPIKGIEKVGVLVK"
     gene            complement(4147..5199)
                     /gene="ricT"
                     /locus_tag="PLI30_04105"
                     /pseudo
     CDS             complement(4147..5199)
                     /gene="ricT"
                     /locus_tag="PLI30_04105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008155403.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory iron-sulfur-containing complex
                     subunit RicT"
     gene            complement(5356..6468)
                     /locus_tag="PLI30_04110"
     CDS             complement(5356..6468)
                     /locus_tag="PLI30_04110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005943253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HOI78772.1"
                     /translation="MYFRNIIGLHDVKKHLTDSVQRGFIPHARLFHGPEGVGKLPLAI
                     AYARYLNCEDRTLEDSCGKCASCHKFDKLMHPDLHFVFPVVKSKVSDEYLPEWREFLS
                     GQTYFNLNDWLNFINAENAQGIIYSKESDEIIRKLSLKVYEALYKIMIVWLPEKMHDA
                     CANKLLKMIEEPPEKTVFLLVSEDLDHVLPTIQSRCQPLNIRAVEPDEMVASIRSVYG
                     LQEEEAKYVTHIANGSFSKAVSIIQSADDNKYFFSLFKEMMRSSVSRNIKAIKSVAGE
                     MASMGREMQKSYLLYSLRLFREFFISNFNEPDMIYLSEEEKEFGERFAPFINEGNIQS
                     FNDEFQLAYRQIEQNGNAKIIFLDLCLKGTMLLKNK"
     gene            6866..7513
                     /locus_tag="PLI30_04115"
     CDS             6866..7513
                     /locus_tag="PLI30_04115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004302161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF47 family protein"
                     /protein_id="HOI78773.1"
                     /translation="MNNSFFSKFTPKEPKFFPLLKGIVEVIKSASYLMLEFVEKGNHA
                     NAADYYKKIKDEERKGDTLSNKIFDELNATFITPFDREDIHNLATKMDDVTDYINSAA
                     KKVFLYNPKEMPAAAKDMVELIIEASAQIEKAVNELDVLKKSRKRVSDYCRELHLIEN
                     RADDVYEHFLIDLFENQKESIELIKKKEIMFELEKATDAAEGVGKIIKTIIVKYA"
     gene            7571..8602
                     /locus_tag="PLI30_04120"
     CDS             7571..8602
                     /locus_tag="PLI30_04120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008618117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HOI78774.1"
                     /translation="MTLLIVIISLALIFDFINGFHDAANSIATIVSTKVLTPFQAVLW
                     AAMFNFVAFFIAKYLIGGFGIADTVSKTVIAEFITLPIIFSGLVAAITWNLLTWWLGI
                     PSSSSHTLIGGFAGAAIAKWASFSVIKVSVIVKIASFIFIAPLVGMLVAMILTIVLQW
                     SVKRANPHKANTWFKHFQLVSSALFSIGHGLNDSQKVMGIIAAALIATHIQHPEIGFG
                     IESISDLPNWVAFSCFFAISLGTMSGGWKIVKTMGTRITKVTPFEGVVAETAGAFTLY
                     LTEFLKIPVSTTHTITGAIIGVGSVKRLSAVRWGVTRKLLVAWILTIPVSALIAATVY
                     FTAGKFLYF"
     gene            complement(8607..>9246)
                     /locus_tag="PLI30_04125"
     CDS             complement(8607..>9246)
                     /locus_tag="PLI30_04125"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005639238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0006555 - methionine metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HOI78775.1"
                     /translation="TEYALIDLSFLGVTDLLLLRGDTKKLDADQRKMDCHSYATGLQE
                     QVNNFNNGIALDGSTFPAPETPFTYGMACYPEKHEESPNMESEIYYTKMKAQNGADYL
                     VTQMFFDNQKYFDFVDRCRAEGITIPIVPGIKPIHLKNQLNVLPKIFRTEIPEDLEHE
                     LRKCKTDADAVEVGVEWCLLQSRELVQRGVPGIHFYALGATESIYRVARELY"
BASE COUNT         2763 a         2014 c         1790 g         2679 t
ORIGIN      
        1 cgttcggggc aggggcttat attttgcaac aggccatatt ctgaaaatgg acctgagtaa
       61 cagttacatt cgtgttagca gtcatctttt caactgttgg atgaggcaac cacacgagga
      121 aaacaaattt ttctaaaaat ccccgggaaa acactccagt acaataaatt gcattatatt
      181 tgtacagttt ttcatagaga atgaaaatca attcaaaaat acggtacgga cttagaacaa
      241 tgatcgaaat tgcttgttgt aaagaaccaa caggcatatt gcaaaaagat attgccaaaa
      301 atcaaaatat ttctgtgaaa tatttggatt acatcataag tgccctcaaa ctgaaaggat
      361 tgatttcaaa tacaaaagga cgaggaagtg gttatatgct ggcccggcca gccgataaaa
      421 taacaatgtt ggacatttac acggcttttg agcaaatagt gataatagaa tgtgtgaaaa
      481 acaggagttt ttgcgaaaga gccacacacc aatgtacagc caataattac tggaaacaat
      541 ttcatcatca gataattgaa attctttcag ctaaaacact tgagcagatt ataaaagaaa
      601 ctaaatacga ttgtcctgaa atttgtgaat aattttttaa aaatgaatcc cactaaacct
      661 ataggataga ttgatattgc atttttttca cgcattatcc tactaatcct ataggattag
      721 tttatccaaa caagaaagag taagtaaata ggcttgtctt aaaaaattcg gaccgacttc
      781 tcagctattt aaagttaaat gtctgaatga gatgaattga taaatgtatt cctactaaaa
      841 ctatagtata actaaacatt aacgatttac aaacttttta ataataaata cagaaacaat
      901 gaaaagtaat tttttattca aatttccaac tgtgttgatc gcatttattt ttgttttaac
      961 aggaacaacg gttaaagctc aaacattgaa aatcaaatca tcaactatta ctatttccgg
     1021 aacaacaaat gttcacccct acacaaccac gtcgactcag gcatcggggg aattaactgt
     1081 ttcaaacaac aaagccacag ttcttaacgt gaatgttccc gtaaagtcaa taatcaacgg
     1141 ggagaaattg atggacaaaa aaacgcatga aacatttaac gaacccaaaa acccatttat
     1201 caactttaaa atgtctgaag taaaatccat tacaacaaac gggtctgaca ttagtgtggt
     1261 tataggtgga acgcttgcaa tggccggcac atctaaaaca gttactcttg aggctaatgg
     1321 caaagaaata aaaccgggaa cgtatacttt tgaaggtagt ttgcccctta aaatgagcga
     1381 ctacaatatg aaagctccaa cggcaatgtt aggagtgatg aaagtcggtg atcaggttac
     1441 ggtcaaatac aacgtaactt ttgaagggcc tgccgttcaa ttcaactaaa acaaaccaaa
     1501 ttatttactt aatatttaaa ctaacgaaaa ctaatgaaaa tgaaacagag aggtttactt
     1561 ctggcagcta ccttgttagc ttcgggcatg atgttcgctc aattgcgtcc tacaaacaaa
     1621 gacggaatca acgtttttga aacaccaaaa accgaaaccg aatttgaagg tttcaatgta
     1681 caattaggcg gtgctttgac acttccgttc tccatgctcg accacagcaa tactgtgaca
     1741 agagaaagtg gctatagtta cgattacgca aacccggcag ctaactctct ggttcccttg
     1801 accagtggct ttggcttacc tcaggccaat ctttacatca agtcaaattt atccgatggc
     1861 atttacttga attttgaact ctatctggct tcaaggcatc acaacgaaac gtgggtaaag
     1921 ggaggttttc ttcaatttga aaaaatggag ttcctacctt gggattttgt ggatgaaatc
     1981 atgaaataca ccaccattaa agtgggtcaa tttgatgtaa actacggtga cgcccacttt
     2041 cgtcgttctg atggcgggtt gacattttac aaccccttta tggaaaacca catcatggat
     2101 gaatttgcca ccgaaattgg cgctgaggtt gatgttcacg tcggagattt tatccttgta
     2161 ggagctgtaa ccaacggcaa actcaacaac gatctgacaa aaattgacac tacgcgtgct
     2221 caaaccaaat attcaaacgg tgtacataat cctgcctgga ttggaaaact ggcttacgac
     2281 aaacaaatca acagccagtt caggctacgt gcaagcgcat cgggatatta cacagccgga
     2341 tccgtaagaa atacactgtt tggcggtgat cgtacaggtt caaactacta cggagtaatg
     2401 tacaatgccc caatatccaa cgcaaatttc acaaacggac gtttcaatcc cggttttggg
     2461 gacaaggtag ccgctgtaat gggcaacttg tttttaaagt tttctcctgt ccagggattt
     2521 tcactcgaat catttacaac actggagaat gcaggcggga gaggtgctaa cgagcctgat
     2581 gtgagaaaat ccaatcaatt tgtaacagat ttggtggtcc gcttcggagc cgatgaagat
     2641 ttttatgtag gtgcccgtta taacacaatg aaagccgaca tgggagctgc acagggtgaa
     2701 cccaatcatt acgaagtgga tataaatcgc gtagccattg cagccggttg gtatatgaca
     2761 aaaaacgtta tggctaaaat tgaatatgta aaccaaaaat acaacggatt tccagccaga
     2821 tccatacaag atggtgcaga gttcaacgga ttgactttgc agggatccat agcattttaa
     2881 gaacaataaa aaccaagccg gtaaattgat accggtttgg ttctttcttt aaaaaattga
     2941 caatgaaagc tttatttgcc ctcttactta taggtatcct gactacagga gctgttgtcg
     3001 cgcaaacatc ttcagctgta gttgctgtag acgtgaataa aaacagcagt ttatcgataa
     3061 acggcagctc gaatattgtt aatttcacac ttacccaaag cagtgaaaac ttcatacaaa
     3121 agaacatgat catcactgca tcacaaaacg ataaaaaact ttacctgagc gaaaaccggc
     3181 ttattgtacc ggtgaaaaga tttacttcaa gcaataagat ggctctcagg gatttttata
     3241 aactattgaa atccgacgaa tacccaacga tggatattca gctgaatcac atagacttac
     3301 ccgaaaatcc aaagctcgcg acaggcgtgg ccgttgtaga tgttaccatt acgggagtta
     3361 caaaaaaata ccaattcccc attaaaaccg aaaaaaacgg tacgagatac atttttgaca
     3421 ttgaaaaaca tataaatatt cgtgacttcg gactggagcc tcccgtacaa atgatgggta
     3481 tgctaaaggt agacgaaatg atcaatatta acttgaaaat gatatgcaac atccagccgg
     3541 cggaaagtgc acaattaaat ttatccggca gttcagaata agccatacac ttattttacc
     3601 aacaccccca ctttctcaat acccttaatg ggattgtctt tcatcacctg acggatgcgg
     3661 ataagataag cacgtgcgga atccagagct acggatgttt tgtagggaac agacagctga
     3721 taaagccccg cagtaccttt tcccagccat tttccttgag gatcagccag tgttatctcg
     3781 acagtgtcgc caacaaatgt agtatctgtt atgttctgtt gaacgagcaa ccagatgttc
     3841 tggtaaggat accggttgtt gttaacgatc tccaacacga tatcgtatcg tttatccgga
     3901 tcaacggaaa gcgagtcgat taaaaaatta gctgtggcct gcttgctcca gctgctgttt
     3961 ttgatcggat gaaaacgata ataaacctct ccttcgtcac acgaagataa gcaagcaaaa
     4021 acaactaaaa aaccaacaaa cagcttactc ctcattttgt ttaggcggtt ttcctttcct
     4081 gtttctgttg tcctgtctac ccctccttgg ttttctcctt ttagaagtac ttccatcatc
     4141 ctttttatca aaacgggtaa tactctgatc tcccagtaaa tcgttctgga tagtttcttc
     4201 ttttgtttca ccggactcag tttcagacac taactttgaa ggttttttcc cttttttatt
     4261 ccactcaata atttgaaata ctctctcttt tgatattgtc tccaagttta tcggattatt
     4321 cttatccgtg gaatacgtga tctgacccga taaaatatcg gtcttgaagt gaaaataatc
     4381 actgtctttg gtctgcagca caatctcacg cgacggtaac cttctgctgg cttccacgta
     4441 tgcatccact tcataattca tacagcattt caattttcca cattgtcccg cgagtttttg
     4501 tgggttaaag gagatatcct ggtaccgggc agctgaagta gataccgata caaaacttga
     4561 catccaactg ctacaacata actcgcgtcc acaaggtcca actcccccta tccttccggc
     4621 ttcctggcgc gcgccaattt gcttcatctc aatctttacc ctgaattccg cagcaaaatc
     4681 tttgattaat tggcgaaagt ccacgcgttc atcagcaata tagtaaaaaa tggccttact
     4741 tccatccccc tgatattcaa cgtctccaat tttcatattc aagccaagac tctcagccag
     4801 ctctctggaa cgaatcatgg taggttgttc tttagcttta gccagctcat acttttccat
     4861 atctgccgga cgcactaacc ggtaaacccg tctcgggcca ctattgccat cttctcgata
     4921 gttgttttta cgcatctgtg cctccaccag ctttccggta agtgttaccg ttccgatatc
     4981 gtgaccggga gaagcttcaa cggccacaac atcgcctttt ttcaaatcga tattgttgct
     5041 gtttatgtaa tatcctttcc gggtattttt aaactgaact tctaccatat tggtcagttc
     5101 ctgcatttcg gggaaatcgt gcaaccaatc ataaacatga agtttagtgg aatgtttggg
     5161 acatcccttg caagaaccac tgtttcgtac agtcgttatt ctttcaggga caatgtccgc
     5221 taatttatct gataattttt ctctgtccat aatatataca ttgatattgt ctaagggcaa
     5281 acgttattta actgcgtgta attcaattgt tgcccgctca cttcgttcgc gttactcgct
     5341 ccgtggaata ttgaattact tattttttaa aagcattgta ccttttaaac ataaatctaa
     5401 aaaaataatt ttggcatttc cgttttgctc tatttgccgg taggcaagct gaaactcatc
     5461 gttaaacgat tggatgtttc cttcgttgat aaaaggcgca aaacgttcac caaattcttt
     5521 ttcttcctcg ctgagataaa tcatgtcggg ttcattgaag ttacttatga agaactcccg
     5581 aaacagacgc aggctgtaca gcaaatagct tttctgcatt tccctgccca tagaagccat
     5641 ttcacccgca accgatttta tcgctttaat gttcctcgat accgaagagc gcatcatctc
     5701 cttaaacaat gaaaaaaaat atttattgtc gtcagcagac tgaataatac tcacggcttt
     5761 tgaaaaactc ccgttggcga tatgcgtcac atactttgcc tcctcttcct gtaaaccata
     5821 aaccgaccgg atagaggcca ccatctcatc cggctctact gcccggatgt tcaacggttg
     5881 gcaacgcgac tgaatagtgg gcagcacatg gtccaagtct tctgaaacca gcaggaaaac
     5941 agttttttcg ggtggttctt caatcatttt cagcaacttg ttggcacatg catcgtgcat
     6001 cttttcaggc aaccatacaa tcattatctt atatagggcc tcgtaaacct tcaggctcag
     6061 tttgcgtata atctcatcgc tctcctttga gtaaataatg ccctgtgcat tctcggcatt
     6121 tatgaagttg agccaatcgt tcaggttgaa ataagtctgt ccactcaaaa actcacgcca
     6181 ctcgggcaaa tattcatcgc taactttcga ttttaccacc ggaaacacga agtgcagatc
     6241 ggggtgcatc agtttatcga atttgtgaca cgatgcacat ttaccacagg aatcttccaa
     6301 cgttctgtct tcgcagttca ggtatctggc ataagcaatg gccaacggca gttttcccac
     6361 accttccgga ccgtgaaaca accgggcatg cggaataaag ccgcgctgca cggaatcggt
     6421 caggtgtttt ttaacatcgt gaagccctat gatgttacga aaatacatgt atcctccgtt
     6481 cttgattata caaaagtaat gaaaaagagc gaaactgaaa agcgtgtcaa aaaaacgttt
     6541 tgtacccctt tctgcaagcc ttttaattgt ccgttaacgg attgttgtat ttttatcctc
     6601 atcgtataaa ctgttttaat ttttgtcatg ccatgcttta tacttatttt ttgtttcatg
     6661 cgtacttttt gatggatttg gattttcaat tcgcacatag tagcgggtta ttttgaagaa
     6721 atttaaaatc caaaacaatt tcttaactca aaattaaaaa aatgaagtaa ttttgcgtga
     6781 ttttttgctt gtagactcgc gttgatcaga aaaatctgcg cctgttccgc gaccgttttc
     6841 tgaaatttat agatacaaaa ggacaatgaa taattcattt tttagtaagt tcaccccaaa
     6901 ggagcccaag ttctttccgc ttttgaaggg gatagtagaa gtgataaaat cagcatctta
     6961 tttaatgttg gagtttgtgg aaaaaggcaa tcacgctaat gctgcagatt actacaaaaa
     7021 aataaaagac gaggaacgca aaggggacac cctttccaac aaaatttttg atgaactgaa
     7081 tgctactttt atcacaccct ttgatcgtga agatattcat aacctggcaa ccaaaatgga
     7141 cgatgtcaca gattatatca acagcgctgc caaaaaagtt tttttgtata atcccaaaga
     7201 gatgccggct gcagccaaag atatggtcga actgattata gaagcatctg cccagataga
     7261 aaaagccgta aatgaattgg atgtcctgaa gaaaagtaga aaaagagtat cggattattg
     7321 ccgggaacta catcttattg aaaacagggc tgatgatgtt tatgaacact tcctaatcga
     7381 cttatttgaa aatcaaaaag agagcatcga gttgataaaa aagaaagaaa tcatgtttga
     7441 gcttgaaaaa gccactgatg cagctgaagg cgtagggaag attataaaaa cgattatcgt
     7501 aaaatacgcg taaaagagtt attacctggc tgtttttcat tctattttat cttaacttat
     7561 aataatccgt atgacgttat tgatcgttat tatttcctta gcgcttattt ttgattttat
     7621 aaatggcttt cacgatgcgg ccaactccat tgccaccatc gtttccacca aggtgctgac
     7681 tccctttcaa gccgttctgt gggcagccat gtttaacttt gtcgctttct tcattgccaa
     7741 atacctcatc ggtggcttcg ggattgccga caccgtttca aaaaccgtga ttgccgaatt
     7801 catcactctt cccattattt tttccgggtt agtggcagcc attacgtgga atttgctgac
     7861 ctggtggctt gggattcctt cgtcctcttc gcatacattg attggagggt ttgccggcgc
     7921 ggccattgca aaatgggcca gtttttcggt catcaaggtt tccgtgattg tgaagattgc
     7981 ttcctttatt ttcattgcgc ccctggtagg gatgctggta gccatgattc ttaccatcgt
     8041 cttgcaatgg tctgtaaagc gtgcgaatcc gcacaaggcg aatacctggt ttaagcattt
     8101 ccagctcgta tcatctgccc tgttcagtat cggccacggg ctgaacgact ctcaaaaagt
     8161 aatgggcatc atcgccgccg cattaatcgc cacccacatc cagcaccctg aaataggatt
     8221 cggaattgag tccatcagcg atttacccaa ctgggtagca ttctcctgct tctttgccat
     8281 ctcgttgggc acgatgtccg gtggttggaa gattgtcaag actatgggaa cacgcatcac
     8341 caaagtaact ccttttgaag gagttgttgc ggaaactgcc ggggcattca cactgtacct
     8401 caccgagttc ctaaaaattc ccgtaagtac tacccacacc atcacaggtg caatcattgg
     8461 tgttggttcg gtaaaacgcc tatcagctgt ccgctggggg gttacccgga aactacttgt
     8521 tgcatggata ttaactattc ccgtcagcgc actgattgcc gccacagttt attttacggc
     8581 aggaaagttt ctctatttct agtacatcaa tacaactccc gggctacccg gtaaatactt
     8641 tctgttgccc ccaaagcata aaagtggatt ccgggcacac cccgctgcac caactccctg
     8701 ctttgcaaaa ggcaccactc cactcctacc tctacggcat cggcatcggt cttgcatttg
     8761 cgcagttcat gctccaggtc ttcgggaatt tctgtcctga atatcttggg aaggacattc
     8821 agctgatttt ttaaatgaat gggctttata cccggaacga tgggaatagt aattccttcg
     8881 gcgcggcaac gatcgacaaa atcaaagtac ttttgattat cgaaaaacat ctgcgtcaca
     8941 agatagtcag cgccattctg agctttcatt ttagtataat agatctccga ctccatgttg
     9001 ggtgactctt cgtgtttctc gggataacat gccatcccat aggtaaacgg agtttcgggc
     9061 gccgggaacg tagagccgtc caaggcaatc ccgttgttaa aattattcac ctgctcctgt
     9121 aatcctgttg cgtaagagtg acaatccatt tttcgctggt cggcatccaa ttttttggtg
     9181 tcaccgcgca acagcaacag gtcagtaacg cccaggaatg aaaggtcaat caacgcatac
     9241 tccgtt
//
LOCUS       DAOSDR010000069         9223 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrimonas sp. isolate AMR_MDS_1826
            NODE_2793_length_9223_cov_4.258726, whole genome shotgun sequence.
ACCESSION   DAOSDR010000069 DAOSDR010000000
VERSION     DAOSDR010000069.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863355
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrimonas sp. (wastewater metagenome)
  ORGANISM  Petrimonas sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Dysgonomonadaceae; Petrimonas.
REFERENCE   1  (bases 1 to 9223)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9223)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:10:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,720
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 35
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9223
                     /organism="Petrimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2793_length_9223_cov_4.258726"
                     /isolate="AMR_MDS_1826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2023866"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..293)
                     /locus_tag="PLI30_04130"
     CDS             complement(<1..293)
                     /locus_tag="PLI30_04130"
                     /inference="COORDINATES: protein motif:HMM:NF025006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HOI78776.1"
                     /translation="MNKKIIYSIIAGMLISTMVVFSQRGHHVLTGKITDENNNELVGA
                     TVYIEELGVGDDTNASGDYRIEHISGGRHRIMVSYMGYKPLTKEIDFKRGEQTR"
     gene            697..1830
                     /gene="sucC"
                     /locus_tag="PLI30_04135"
     CDS             697..1830
                     /gene="sucC"
                     /locus_tag="PLI30_04135"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006952195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HOI78777.1"
                     /translation="MKIHEYQARELLASYGLPVDKAYICRNVDDAVRAYQELDTPLVV
                     VKAQVHTGGRGKAGGVKLAKNEIELRQHVANILWMDIKGFIVDRVLIGKAVNIASEYY
                     ASYVIDRKSKSTILMLSREGGMDIEEVARNTPEKIHKIVIDPLIGIPDYLAREAAFKL
                     FDDIKLVREAATIFQKLYRLFVDTDASLAEINPLVLTEEGLIKAIDAKMTFDDNALYR
                     HPKIAALFEPTEEEKKEQNAKSKGFSYVHLGGEIGCMVNGAGLAMATMDMIKLYGGEP
                     ANFLDIGGSSNPTKVIEAMKLLLSDKNVKVVLINIFGGITRCDDVARGLLEAFSQIKT
                     DIPIVIRLTGTNEKEGRALLEGTEFHLAETMGEAGKKAVELAN"
     gene            1863..2729
                     /gene="sucD"
                     /locus_tag="PLI30_04140"
     CDS             1863..2729
                     /gene="sucD"
                     /locus_tag="PLI30_04140"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014709327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HOI78778.1"
                     /translation="MSILIDKNTRLIVQGITGRDGGFHTAKMKAYGTNIVGGTSPGKG
                     GENVEGIPVFNTVREAVKETEANTSVIFVPAPFAKDAMLEAADAGVKLIICITEGVPT
                     LDVVEAYRYINLKGAKLIGPNCPGLISPDKSSVGIMPTMIFKRGATGVISRSGTLTYE
                     VVYNLTSKGLGQSTAVGVGGDPIVGLYYQELLEMFQNDPETDSIALIGEIGGDAEERA
                     AEYIKKHVTKPVAAFIAGQQAPPGKQMGHAGAIISSGSGSAAEKIAAFEAVGVPVAKE
                     PSQIPELLKSRL"
     gene            complement(2724..3617)
                     /locus_tag="PLI30_04145"
     CDS             complement(2724..3617)
                     /locus_tag="PLI30_04145"
                     /inference="COORDINATES: protein motif:HMM:NF012442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HOI78779.1"
                     /translation="MENRITLANLADLLAKRTNVSGTESEAFLKSLFELISETLSGDE
                     VVKIKDFGTFKLVPIQARESVDVNSGEKIEIPAHNRLSFFPATALKELVNKPFSHFES
                     ILLNEGIAFEGVEEVIEEEEGKTELTEEKLIVKREAYQEVFRSDRMASANVQKRSKIP
                     PVWIPILGGVAIALASLFFFIQRQAGTQPATITENPSVKDSLHPFKAPVAEKTQELPE
                     RLEKVVVQKGKTLRLIAEEKFGNREFWVYIYLKNKDKIENPNVVTVGTELIIPDSSEY
                     DIDASDTQSVAKAKVLGDDFL"
     gene            complement(3607..3900)
                     /locus_tag="PLI30_04150"
     CDS             complement(3607..3900)
                     /locus_tag="PLI30_04150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006842170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HOI78780.1"
                     /translation="MTHKELISEMAQRLGLSQSKVSDLLDATVCELNEKLSENTQIFI
                     PHFGVFETRKRSERISVNPQTQQRYLVPPSIVATFKPASGIKEQLKTMEEDGK"
     gene            complement(3897..5192)
                     /gene="rimO"
                     /locus_tag="PLI30_04155"
     CDS             complement(3897..5192)
                     /gene="rimO"
                     /locus_tag="PLI30_04155"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007656545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0018339 -
                     peptidyl-L-beta-methylthioaspartic acid biosynthetic
                     process from peptidyl-aspartic acid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HOI78781.1"
                     /translation="MKNRIDVITLGCSKNLVDSELLMRQLVANGYTVEHDPADPKGDI
                     AVINTCGFIGDAKEESVNMILEFAEAKKTNRLKKLFVMGCLSERYMNELQNEIPEVDK
                     FYGKFAWKNLISDLGKSYYKDLEFDRSLSTPPHYAYVKISEGCDRTCSYCSIPIMTGK
                     YKSRPLEEIEEEVRRLVSQGVREFQLIAQDLTYYGLDRYKQMRLPELVERVADIKGAD
                     WIRLHYAYPAHFPIELLRVIRERENVCNYLDIALQHISDNMLGRMRRNITKQQTIDLI
                     ARFREEVPGIHLRTTMMVGHPGETEQDFEELLEFVRETRFERLGAFPYSHEEDTYCDN
                     NYTDDVPPGLKQERMNVLMDVQESIAVTINESKVGKTLKVIIDREDPDYYVGRTEFDS
                     PEVDGEVLIEKQSPLRTGEFYEVKITSAMPFDLIGNVSL"
     gene            complement(5189..6142)
                     /gene="ftsY"
                     /locus_tag="PLI30_04160"
     CDS             complement(5189..6142)
                     /gene="ftsY"
                     /locus_tag="PLI30_04160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005857252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HOI78782.1"
                     /translation="MGLFEIFSKKKKETLDQGLEKTKENIFSRLTRAVAGKSRVDDDV
                     LDELEEILVTSDVGVDTTLKIIDRIEGRVARDKYVSTDELTKILREEIAELLTENNSG
                     DTVEFAVPENKKPYVIMVVGVNGVGKTTTIGKLAYQFKQNGLSVYLGAADTFRAAAVE
                     QLDIWGKRVGVPVVKQKMGADPASVAFDTLSSAKANDADVVIIDTAGRLHNKINLMNE
                     LTKIKNVMSKVVPGTPDEVLLVLDGSTGQNAFEQAKQFTAATEVSALAITKLDGTAKG
                     GVVIGISDQFKIPVKYIGLGEGMEDLQVFRRKEFVDSLFGE"
     gene            complement(6285..6446)
                     /locus_tag="PLI30_04165"
     CDS             complement(6285..6446)
                     /locus_tag="PLI30_04165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007214255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4295 domain-containing protein"
                     /protein_id="HOI78783.1"
                     /translation="MAKKAVAGFRDKTSTTGRSHTKVIKMVKSEKTGAYSFREEMVLN
                     DRVQDFFKK"
     gene            complement(6461..6649)
                     /gene="rpmG"
                     /locus_tag="PLI30_04170"
     CDS             complement(6461..6649)
                     /gene="rpmG"
                     /locus_tag="PLI30_04170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012573149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HOI78784.1"
                     /translation="MSKKAKGNRVQVILECTEHKESGMPGTSRYITTKNRKNTTERLE
                     LKKYNPILKKMTVHKEIK"
     gene            complement(6669..6908)
                     /gene="rpmB"
                     /locus_tag="PLI30_04175"
     CDS             complement(6669..6908)
                     /gene="rpmB"
                     /locus_tag="PLI30_04175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005857630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HOI78785.1"
                     /translation="MSKICQITGKRAMVGNNVSHSNRRTKRKFNVNLFKKKFYWVEED
                     CWISLNISASGLRTVNKIGLDAALKQAAEKGYLNA"
     gene            complement(7042..7611)
                     /locus_tag="PLI30_04180"
     CDS             complement(7042..7611)
                     /locus_tag="PLI30_04180"
                     /inference="COORDINATES: protein motif:HMM:NF026080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4468 domain-containing protein"
                     /protein_id="HOI78786.1"
                     /translation="MKKIIFTALIFASGLIQVNAQFVFTAVPVVNGKVVFQQFIHIDR
                     ELAADQRYALLYKWGKDNYAGNPLLSGIRFDDKARSITVGSKIELLLPQNSNGVREKV
                     VMNYRFDATITNAGCMLVVRDVTYQNSQSPNSSFFPKTFTAEETITSTAISAASGLDK
                     EFKTNTQKSTLFYLNGLYNELSKIFNLSK"
     gene            7792..8493
                     /locus_tag="PLI30_04185"
     CDS             7792..8493
                     /locus_tag="PLI30_04185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019539515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOI78787.1"
                     /translation="MSNKHQESKAEKGFENIEEALTSSERFIEKNQKNILIALAAVVL
                     VVGAIIAYNYLYKNPRNEKAQAAIYKGERFFQNQEDSLALFGNGNDFIGFENIIKDFS
                     GTKTADLARAYAGISYSRLGNNEKALEYLNKFKGGDLLITPAIAGAIGDVYMNMGDTE
                     KAISHFNNAAQKANDEMLTPIYYNKVGLAYLSVKNYDKAIETFQMIKDKYLNSPQGQE
                     ADKYIEAAKLQKAGN"
     gene            8493..8984
                     /gene="ribH"
                     /locus_tag="PLI30_04190"
     CDS             8493..8984
                     /gene="ribH"
                     /locus_tag="PLI30_04190"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019539514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HOI78788.1"
                     /translation="MATELQNLSSYDPGSTPRGEGKKIGIVVSEWNKPVTGNLLNGAI
                     QTLLKFGVQQNDIVVEYVPGSFELTYGAKKLIEKIDPDSVIVIGCVIQGETPHFTFVC
                     NSVTQGITELNILHNIPVIFGLLTTNTLEQAKARSGGRHGNKGDEAAITALKMIALNE
                     KYK"
     gene            9014..9096
                     /locus_tag="PLI30_04195"
     tRNA            9014..9096
                     /locus_tag="PLI30_04195"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9048..9050,aa:Tyr,seq:gta)
BASE COUNT         2661 a         2073 c         1842 g         2647 t
ORIGIN      
        1 cgggtttgct ctccccgttt aaaatctatt tctttcgtaa gcggtttata acccatataa
       61 ctcaccataa tccggtgtcg tccgccggaa atatgctcga tacggtaatc ccccgacgcg
      121 ttagtatcgt ctccgacgcc caattcttca atataaacgg ttgctcccac taactcattg
      181 ttgttttcat ccgttatttt ccccgtcaac acatggtgac cgcgttggga aaaaacaacc
      241 atcgtgctta tcagcattcc ggcaattatt gagtatatta tttttttatt catctttttt
      301 cttcttgatt tttcagagca caaaggaaga gcaaaatcac gcttcgcaca atcccataaa
      361 tcagggatat ttcgataaac agaataccta aaaaaagtga ttatctcacg cgagctatca
      421 cagtacttct gtatcaaagt tctatcagaa aaaattgaat ctaatgaaat tcaaaaaagc
      481 tttccaacaa tacaataata tactgataaa cattttgtta ttctattttt ttacactttt
      541 tgccgggtac ggaaattatt tttctctatt tgagcaaaca ttttaccgct ctctcatgtt
      601 aaacaacata attaaagtgc cacatattgt cagaaaaatc atacagctac atcaaaaatc
      661 aatcattcat taaatattag tataaaataa aagcccatga aaattcatga gtatcaggca
      721 agagaattat tagcatctta cggattgccg gtagacaaag catatatctg tcgtaacgtt
      781 gatgacgcag tgcgcgcata ccaggaactg gatactccgt tagtggttgt aaaagcgcag
      841 gtccataccg gaggtagggg gaaagctgga ggggtgaaac tcgccaaaaa tgaaatagaa
      901 ctccgccaac atgtagccaa catcttgtgg atggatatta aaggctttat cgttgacagg
      961 gtgcttatcg gtaaagcggt gaatattgct tcggaatatt atgcaagcta tgtaatcgac
     1021 aggaaatcta aatctactat cctgatgttg agtcgcgaag ggggaatgga tattgaagaa
     1081 gtggcacgta acactcccga aaaaatccat aaaatagtta tcgacccgct tatcggtata
     1141 ccagactact tggcacgaga agctgctttc aaactgtttg acgatatcaa gctagtccgt
     1201 gaagcagcaa caattttcca aaagctatac aggttgttcg tggataccga cgcatcgctg
     1261 gcagagatca accctttggt gctcaccgaa gaaggactga ttaaagccat cgacgcaaaa
     1321 atgactttcg acgacaatgc cctttaccgt catcctaaaa ttgcggctct ttttgaaccg
     1381 acagaagaag aaaagaagga gcaaaacgct aaaagcaaag gtttcagcta tgtgcattta
     1441 ggcggtgaaa taggttgtat ggttaacggt gccggactgg ccatggcgac gatggacatg
     1501 ataaaactgt atggcggaga accagctaac ttcctggata tcggtggaag ctcaaatcct
     1561 acaaaagtca ttgaagccat gaagctttta ctaagtgaca agaatgtaaa ggtggtactg
     1621 atcaacatct ttggagggat tacccgttgc gacgatgtag ccagaggatt actggaagcc
     1681 ttcagccaga taaaaaccga tattcctatt gttatccgtt taaccggaac caatgaaaaa
     1741 gaaggccgcg ccttattgga aggcaccgaa ttccacttgg cggaaaccat gggtgaagca
     1801 gggaaaaaag ccgttgagtt ggcaaattaa gttcagcttt aaacttttaa cgtctaaaaa
     1861 atatgagtat tctaatcgat aaaaatacac gattaatcgt tcagggaatt accggacgcg
     1921 acggaggatt tcacactgca aagatgaaag catacggcac caatattgtt ggagggacat
     1981 cgcccggaaa gggcggagaa aatgtggaag gaatccctgt ttttaatacc gttcgtgaag
     2041 ctgtaaaaga gacagaagcc aacacatcgg taatcttcgt tccggctccg tttgccaaag
     2101 atgccatgtt ggaggctgcc gatgcaggag taaaacttat tatctgtatt accgaaggtg
     2161 tccctaccct cgatgtagtt gaagcttacc gctacatcaa cctaaagggt gccaaactaa
     2221 tcggtcccaa ttgtccgggt ttaatatctc ccgataaaag ttcagtgggc ataatgccta
     2281 ccatgatttt caaacggggg gcaaccggag taatcagccg gagtggaaca ctcacttacg
     2341 aagtcgttta caacctcacc tccaaagggc tgggacaatc cactgccgta ggtgtaggcg
     2401 gagatcccat tgtggggctc tattatcagg aactgttgga aatgtttcaa aacgatcctg
     2461 aaaccgactc tatagccctt atcggcgaaa ttgggggcga tgctgaagaa cgcgctgctg
     2521 aatacatcaa aaagcacgtt acaaaaccgg tagccgcgtt cattgcagga caacaggctc
     2581 ctcccggaaa acagatggga cacgcaggag caatcatctc cagcggttcg ggtagtgccg
     2641 ctgaaaaaat tgctgctttc gaagccgtgg gagttccggt agccaaagaa ccgagtcaaa
     2701 taccggaatt attgaagagc aggttataaa aaatcgtcgc ctagcacttt ggctttggct
     2761 acagattgtg tgtccgaggc atcaatgtca tattcagaag aatcaggaat aatcagttca
     2821 gtcccaacgg taacaacgtt gggattttct attttatctt tgtttttcag gtagatatac
     2881 acccaaaact cccggttccc gaacttctct tcggcaatca gtctgagggt tttacccttt
     2941 tgcaccacca ctttttccaa cctttccggc agctcctgag ttttttcagc aacaggagcc
     3001 ttgaaaggat gcaaactgtc ttttaccgaa gggttttcag ttattgttgc cggctgtgtc
     3061 ccggcttgcc gctgaatgaa aaaaaaaaga cttgccaacg caatggcaac gcctcccaaa
     3121 atgggtatcc agacaggagg aattttagaa cgtttttgga cattggcgga agccattcta
     3181 tccgagcgaa aaacttcctg ataagcttcc ctttttacta tcagcttttc ttcggtcaac
     3241 tccgtctttc cctcttcctc ttcaatcacc tcttccactc cttcgaatgc gataccttca
     3301 ttcaacagaa tagactcaaa atgagaaaac ggtttgttta caagctcttt taatgctgtt
     3361 gccgggaaaa aactcaaccg gttatgtgcg gggatttcta tcttttcgcc ggaattcaca
     3421 tcaacgcttt cacgggcttg aatcggaacg agcttaaatg ttccgaaatc tttgatttta
     3481 accacttcgt ctccggaaag agtttccgag atgagttcga acaacgattt taaaaatgcc
     3541 tctgactctg taccggaaac attggttcgc ttagccagca aatctgccag gttggcaagg
     3601 gtaattctat tttccatctt cttccatggt tttcagttgc tctttaatgc ctgatgccgg
     3661 tttaaatgtg gccacaatag atggaggaac caggtaccgc tgttgggtct gaggattcac
     3721 ggagatacgc tcagatctct ttctggtttc gaaaactcca aaatgcggaa tgaaaatttg
     3781 tgtattctcc gaaagttttt catttagttc acataccgtc gcatccagca aatcagacac
     3841 cttcgattga gaaagaccca gccgttgcgc catctctgaa ataagttcct tgtgtgtcat
     3901 aatgaaacat ttcctattaa atcaaacggc atagccgatg taatcttcac ctcataaaat
     3961 tcacccgttc tcaatggaga ttgtttttca atcaacactt ccccatcaac ttcaggagaa
     4021 tcgaactcgg tacgacccac gtaataatcg ggatcttccc ggtcgataat taccttcagg
     4081 gttttcccca ctttcgattc gttaatggtt acagcaatag attcctgcac gtccatcaac
     4141 acattcatcc tttcctgttt tagaccagga ggcacgtcgt ccgtataatt attatcgcaa
     4201 taggtatcct cttcgtgcga gtaagggaaa gctcctaaac gctcgaaacg ggtctctctt
     4261 acaaattcaa gaagttcttc aaaatcctgt tcagtctcac ccggatgtcc taccatcatc
     4321 gttgtgcgca ggtgaattcc aggaacttcc tcccgaaaac gggcaatcag atcaatcgtt
     4381 tgttgcttcg taatgtttcg tctcattctc cccagcatat tgtcgctgat gtgctgcaag
     4441 gcaatgtcga gatagttaca aacgttttct cgctcgcgta ttacgcggag taattcgata
     4501 gggaaatgtg ccggataagc gtaatgaaga cgaatccaat cggcaccttt gatgtccgca
     4561 acccgttcaa ccagttcggg caacctcatc tgtttatacc tgtccagtcc ataataggta
     4621 aggtcttgcg cgataagctg aaactcccgt acgccttgcg aaaccaaacg tcgaacttct
     4681 tcttcaatct cttccagtgg ccgtgatttg tacttacccg tcatgatggg gatggagcaa
     4741 taggaacagg tgcggtcgca tccttcggat attttcacat aagcgtaatg cggcggagtt
     4801 gacagggaac ggtcgaactc aaggtcttta tagtacgatt tacctaagtc agaaatcagg
     4861 tttttccaag cgaatttgcc gtaaaatttg tctacttcgg gaatttcgtt ctgcagttca
     4921 ttcatgtacc gctccgataa acatcccatt acgaaaagtt ttttcagcct gtttgtcttt
     4981 ttggcttccg caaattccag gatcatattg acggattcct ccttggcatc gccaatgaaa
     5041 ccgcacgtat ttatcacagc tatgtcacct ttgggatctg caggatcatg ctccaccgtg
     5101 taaccgttag caacaagttg tcgcattagt aattcggaat ctaccaggtt tttagaacaa
     5161 cccagggtta tgacgtctat tctgttcttc attctccaaa caacgaatcc acaaattctt
     5221 tccttcggaa aacttgcagg tcttccatcc cttcacccag ccctatatat tttaccggaa
     5281 ttttaaattg atccgagata ccaataacga ctccaccttt tgctgttcca tctagtttag
     5341 tgatagccag agccgagact tcggtggcag cggtaaattg tttagcctgt tcaaacgcgt
     5401 tttgcccggt ggaaccgtcc aataccagaa ggacttcgtc cggagtgccc ggaactactt
     5461 tactcatcac gtttttaatt ttggtaagct cgttcatcag gttaatcttg ttgtgcaaac
     5521 gccctgcagt atcaatgatt accacatcgg catcatttgc ttttgccgaa ctcaacgtat
     5581 caaaagccac cgacgcggga tcggcaccca tcttttgctt caccacggga acccccacac
     5641 gtttacccca gatatcaagc tgctccacag ccgcagcacg gaaagtatcg gccgcgccaa
     5701 gatatacgga caacccattt tgtttaaact gatacgccag tttccctatg gtagtggttt
     5761 tccccactcc gttcacccca actaccataa taacgtatgg ctttttattt tccgggacgg
     5821 caaactcaac tgtatcaccc gagttgtttt cagtgagcag ttcggctatc tcctcccgga
     5881 gaatttttgt aagttcatcc gtactcacat atttgtcgcg ggcaacacgt ccctctatcc
     5941 ggtcaattat ttttaatgtc gtatcaacac ctacatccga agttaccaat atttcttcca
     6001 gttcatcgag cacatcatcg tctactctcg attttccagc aacggcacgg gttaatctgg
     6061 agaaaatatt ttccttggtt ttttccagtc cctgatcaag cgtctctttc tttttcttcg
     6121 aaaatatttc gaacaatccc atagttgcgt aatttttact ttttcaattt cccatgcaag
     6181 ggcttatgcg atgcttccgc aatatcgtac aaatctacaa aatagtcaat aaaaaacttc
     6241 cccacaagtt cttgcaggga agtttttaca tatttttaac ggatttattt tttaaaaaaa
     6301 tcttgtaccc gatcgttaag gaccatctct tctctgaaac tgtaagctcc tgttttttca
     6361 gacttcacca ttttaataac cttggtgtgg ctgcgtcctg ttgttgaagt tttatcgcgg
     6421 aatcctgcga ctgctttctt tgccatagtt tactccttaa ttatttaatt tctttatgaa
     6481 cggtcatttt ctttaagatg gggttgtatt ttttcaactc caatctttcg gttgtgtttt
     6541 ttctgttctt cgttgtaatg tatctcgaag tcccgggcat tccgctttct ttatgttcgg
     6601 tacattctag aattacctga accctgtttc cttttgcttt tttcgacatt gctctttcct
     6661 cctgataatt aagcgtttaa ataccctttt tcagcagctt gctttaaagc ggcatccaat
     6721 cctattttat taacggtgcg taaacctgaa gcagaaatat tcaggctgat ccagcaatct
     6781 tcttccaccc aatagaattt ctttttgaac aaattaacgt taaatttacg ttttgttctt
     6841 ctgttggagt gagaaacgtt gtttccaacc attgctcttt ttccggttat ttgacaaatc
     6901 ttagacattg ctatgttgta ttttattgat ttctataaat attcagctgt tttcggacaa
     6961 atcagggcgc aaaattaacc ataaaaaatg tatttaccaa aaaaacggca aataaatttt
     7021 caagaagatc gggatccgtc tttatttact gagattaaaa attttactca actcattgta
     7081 caacccgttc aggtaaaaca acgtactctt ttgggtgttc gtcttaaatt ctttgtctag
     7141 acctgatgcg gcagaaattg cagtggaagt aatggtttct tctgcggtaa acgttttagg
     7201 gaagaacgag gaattgggag actggctgtt ctgataagta acatccctca caaccaacat
     7261 acagcccgca ttggttatgg tagcatcaaa ccggtaattc atgaccactt tttcccgcac
     7321 tccgttgctg ttctgcggca acaacagttc tattttagaa cctacggtta tgcttcttgc
     7381 cttatcgtca aagcgtatac ccgataacaa agggttgccg gcgtaattat ccttccccca
     7441 cttgtagagc aatgcatatc gctgatcagc agcaagttcc cgatcaatat gaataaactg
     7501 ttgaaacacc actttcccgt ttaccaccgg aacagcagta aaaacaaact gggcatttac
     7561 ctgaatcagt cctgaagcga atattaatgc agtgaaaata atcttcttca taacttaagt
     7621 tttagtttct tgcataaatt tactttatac aatgtatatg actacaaact tacggaaaag
     7681 attaatcagc gacaaacagt ttagagaaaa tttatccaat ttattttatt tatcgcttaa
     7741 aaaccattac ttttgcaact tgaaatttga taacattatt ttttgaacaa aatgtccaat
     7801 aaacatcagg aaagtaaagc cgagaaaggt tttgagaata tcgaagaagc gttgacttca
     7861 tcggaacgtt ttattgaaaa gaatcaaaaa aacattttaa tcgcattggc agcagtcgtt
     7921 ttggttgtcg gcgcgatcat tgcatacaac tatttgtata aaaacccacg aaacgaaaaa
     7981 gcacaggcag ccatttacaa aggtgaaaga ttctttcaga atcaagaaga ctcccttgcc
     8041 cttttcggta acggtaacga ttttatcggc ttcgaaaaca tcataaaaga tttcagcggc
     8101 accaaaactg ctgacctagc ccgtgcctat gccggaatca gttacagcag attgggaaac
     8161 aatgaaaaag cgctggagta cctgaacaaa ttcaaaggtg gcgatttgtt aatcaccccg
     8221 gctatagcag gagcaatagg tgatgtttat atgaatatgg gggataccga aaaagccatt
     8281 tctcatttca acaatgcagc ccaaaaagcc aacgatgaaa tgctgacgcc catctactac
     8341 aacaaagtgg gacttgcata cttatccgtt aaaaattacg ataaggccat tgaaaccttc
     8401 cagatgatca aggacaaata cctcaactca ccacagggac aggaagcaga caaatacatt
     8461 gaagccgcca agcttcaaaa agcaggaaat taatggccac tgaattacag aatctatcgt
     8521 catacgaccc cggatccaca ccgcggggtg aaggaaaaaa aattggtatt gttgtttcgg
     8581 aatggaacaa acctgttaca gggaatttgc taaacggcgc cattcaaaca ctgcttaagt
     8641 ttggtgttca acagaacgac attgttgtgg aatatgtccc tggaagtttt gagctcacct
     8701 acggggctaa aaaactgatt gagaaaatag acccagacag tgttatcgta atcggatgcg
     8761 ttattcaggg agaaacacca cacttcacgt ttgtgtgtaa cagcgttaca caaggaatca
     8821 ctgagctgaa tattctccac aacatcccgg taattttcgg attactgaca accaatacgc
     8881 tggaacaagc gaaagcacgt tcgggcggga ggcacgggaa caaaggtgat gaagctgcaa
     8941 ttactgcatt aaaaatgatt gcgctcaacg aaaaatacaa ataattgtat tatctttgtc
     9001 cctcgaaaat tttgggcagt taccagagtg gccaaatggg gctgactgta actcagctgg
     9061 cttacgcctt cggtggttcg aatccatcac tgcccacaaa acaattcaca atgcaaaatg
     9121 cacacttatt atgcattctt gatcatacat tgtaaattga atgttgcgga agtagctcag
     9181 tcgatagagc attagccttc caagctgagg gtcgcgggtt tga
//
LOCUS       DAOSDR010000070         9214 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrimonas sp. isolate AMR_MDS_1826
            NODE_2795_length_9214_cov_4.261382, whole genome shotgun sequence.
ACCESSION   DAOSDR010000070 DAOSDR010000000
VERSION     DAOSDR010000070.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863355
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrimonas sp. (wastewater metagenome)
  ORGANISM  Petrimonas sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Dysgonomonadaceae; Petrimonas.
REFERENCE   1  (bases 1 to 9214)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9214)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:10:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,720
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 35
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9214
                     /organism="Petrimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2795_length_9214_cov_4.261382"
                     /isolate="AMR_MDS_1826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2023866"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1697)
                     /gene="sppA"
                     /locus_tag="PLI30_04200"
     CDS             complement(<1..1697)
                     /gene="sppA"
                     /locus_tag="PLI30_04200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019538807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HOI78789.1"
                     /translation="MKDFFKMMFASALGFIIASFIFSLISMLIMFAMMGSIMGSFGKQ
                     ESFVLQNNSVLNLRLEGAIQERIAESDPFTDLVGGVPSAIGMNDIIGAIRKAKNNDKI
                     KGIYLDTRLFAASTATLAEIRQELEDFKKSGKFIVAYADTYTQNGYYLASVADKVAIN
                     PQGMLDVHGIASVPLFYKDALQKLGVEMQLFKVGTYKSFAEPFTQTEMSEANREQVNS
                     FITDIWNTMKTDMAASRNMETMQIDSIANQFPMLRKTDFLLSRNLVDTVLYESEMKNY
                     VRELLGIDTDTKIPSATVAEMKSVKTPAIRKSTNSIALLYATGGIASGNRPNGIQDKY
                     FVNEIEKLRKDDDIKAVVFRINSGGGSAYASEQIWKAISDLKSEKPVVVSMGDMAASG
                     GYYIACNADKIVAQPTTITGSIGIFGMFPNFSGTLDKLGIDTDVVKTNEFSDFGDVSR
                     AFNAQEAAMMQAYVDSGYDLFLTRCAEGRGMPKDSLAKYAEGRVWTGNQAREIGLVDE
                     LGGVDEAIRIAAEMANLGKSYAVFEYPRIRSPFEEIFSKNKEELAAKTLKNYLGESY"
     gene            1966..2523
                     /locus_tag="PLI30_04205"
     CDS             1966..2523
                     /locus_tag="PLI30_04205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019538806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOI78790.1"
                     /translation="MDDSQLITACKKQNRNAQKALYELYAPKMMAVCLRYCQDNETAR
                     DVLHDGFLQVFTQIGSYSGKGSFEGWLRKIFVNLALENYRQEKKRFNFLNDYGKDNSD
                     FWDTPEDDSLEVGDIPRQELLNIIREMPEGYRTVFNLVIFEDIPHKEIGAMLGITENA
                     SRSQYFRAKTFLQKKVQSMLKKKYI"
     gene            2530..3894
                     /locus_tag="PLI30_04210"
     CDS             2530..3894
                     /locus_tag="PLI30_04210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI78791.1"
                     /translation="MNEKDIFKEQLKSVFSDFEAPVPPDGWEKVEQSLNAIRRARIIR
                     RNWYVGSAAATAALILGSLFFLNSPHPVKPDLPVFTGKMAPEKTGSLDTQDTQIAKTE
                     TTKKESNKDSQVGYSLQKQEVLVARYTKRKEKVLSVNIDLEQTPEKIVIAEQTVEVKA
                     EAMGERIIRPEERQVVQPSQEEVDRLIQEFENAGKATIFEGLAFEDKKSKPIMLALNA
                     KGGLTSSRKTVNSPMTLRSASAVKPSENGDGLYSGLTGSNKMYADYSSIPFNNRSIAD
                     NVAEMEHDQPVSFGITVSKTIVDRLSVETGLVYTYLFSRAKNTSVDFQNQETQHFHYL
                     GIPINLNYNFVNIGKLGLFASFGGMVEKDVYGEFRSTGQSVSTELNSASQGVITTKIS
                     QKNPQISVNAGVGVFYPIYGGFNLYGKVGGVYYFDAKNYEYKTIYTDKKIGFDLNAGI
                     RFDF"
     gene            3925..4629
                     /locus_tag="PLI30_04215"
     CDS             3925..4629
                     /locus_tag="PLI30_04215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI78792.1"
                     /translation="MRKLILFFSVVIAGLFLTSCLEAGDSNFSGQRQLFYITQLEGTQ
                     VAHNGDFVMTSNEIKSKDPDRWYLITWSWSTENGMVSTNVHNAITPEIEQIPLGVYSQ
                     QEAPQETMTPVLNFNMVGGLPIAGSFGDFLIVQYVWNKKEGEKVQAKLYNETTQPDAT
                     DTYILELRLEKSGTSTETSGKNVDGFAAFDVSSLRSLVNFNGQDFKDIQLKFNYYKEN
                     AGQPTSVTQPVRIYKN"
     gene            complement(5247..7187)
                     /gene="rpsA"
                     /locus_tag="PLI30_04220"
     CDS             complement(5247..7187)
                     /gene="rpsA"
                     /locus_tag="PLI30_04220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019540257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HOI78793.1"
                     /translation="MTDNLKTVAPIEEFDWDAYAKGDVYSKENKEKLTESYDNTLSKI
                     NDKEVVTGTVTSMNKREVVVNIGYKSDGVVSMNEFRYNPELKVGDEVEVYIESQEDKK
                     GQLILSHKKARASRSWDRVNAALENNEVIKGYIKCRTKGGMIVDVFGIEAFLPGSQID
                     VKPIRDYDIFVDKTMEFKVVKINPEFKNVVVSHKALIEEELEQQKKEIISKLEKGQVL
                     EGVVKNVTSYGVFVDLGGVDGLIHITDLSWGRIQHPDEVVKLDDKINVVILDFDDDKK
                     RIALGLKQLTPHPWDAMDDSLKVGDIVKGKVVVIADYGAFVEVAAGVEGLIHVSEMSW
                     TQHLHSAQDFMKVGDEVEAVILTLDREERKMSLGVKQLKPDPWDNIEEKYPVGSTHTA
                     TVRNFTNFGAFVEIEEGVEGLIHISDLSWTKKIKHPSEVTEIGAPIKVQVLEIDKENR
                     RLSLGHKQLEENPWDVFETIFTAGSVHEGTILEFVDKGAVIALPYGVEGFATPKHLVK
                     EDGSQAQVDEKLEFKVIEFNKDAKRIIVSHSRIHEDAQGGGSDESRKGKRSGKKENQS
                     STMAIPVMEKTTLGDIEELAALKEKLDNQKVAAFKKEAKAKANKEEEAKVAEAEVKDE
                     PKIATEAEETVENKTEETKSEE"
     gene            complement(7346..7891)
                     /locus_tag="PLI30_04225"
     CDS             complement(7346..7891)
                     /locus_tag="PLI30_04225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005855842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1/PfpI family protein"
                     /protein_id="HOI78794.1"
                     /translation="MKKVALFLANGFEEIEALGTVDILRRAGIPVQTVSITNEKNVTG
                     AHHITVLADVIFREVNFHDVDVLILPGGMPGAKNLNEHEELKRKVKEYAEKGKVVAAI
                     CAAPMVLGGLGLLEGKKATCYPGFEHQLTGAKVSSNKVSVDKNIITGKGPGLVFEFAL
                     ELVETIAGAGTRKEVQEGLLI"
     gene            complement(7965..8750)
                     /locus_tag="PLI30_04230"
     CDS             complement(7965..8750)
                     /locus_tag="PLI30_04230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006844533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HOI78795.1"
                     /translation="MQITREKTAGIAGTILFAVLLLLVLLFSYFTLVIPPDELEGIPV
                     MFGNVEEASGTTESPMNEISPAPAQRPSVPEYSPEEPLITQQTEPTIDVTAQREAERR
                     KAQLAEQRRLQEEAARRQREEDARRREINQQMSGLFGENSGSRGETEGSGTQGVSTGN
                     ASQGATSGTGGIGTFDLGGRSLGSGGLVQPNYSVNDYGTVVVNITVDPRGNVINAEIG
                     RGTNTPNTSLRNEALRAARRTKFNAINSANNQQGTITYRFNLN"
     gene            complement(8776..9183)
                     /locus_tag="PLI30_04235"
     CDS             complement(8776..9183)
                     /locus_tag="PLI30_04235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019540261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HOI78796.1"
                     /translation="MALKQRFNVEANFSMASMTDVIFLLLIFFMITSTIVVPNAIKVL
                     LPKSQQQAQAKPITRVTIDKSLNYYVANANETERMVTFEQITPFLQSVYSAEPDIFVA
                     LYADEEVPYREIVKILDIANQNQFKMVLMTRPN"
BASE COUNT         2471 a         1951 c         1912 g         2880 t
ORIGIN      
        1 tcgtagcttt ctcccagata attctttaac gttttggcag ccaattcttc tttgttcttg
       61 ctgaaaattt cctcaaacgg tgaccttatc cttggatact cgaaaacagc atagctttta
      121 cctaaattag ccatttcagc cgcgatacgg atagcttcat cgaccccgcc cagttcatct
      181 accaatccaa tttccctggc ctgatttcct gtccaaacac gaccttctgc gtatttggcc
      241 aggctgtctt ttggcattcc acggccttcg gcacatcttg tcaggaacag atcgtaaccg
      301 ctgtccacat aagcttgcat catcgctgct tcctgagcgt tgaaagctcg ggaaacgtca
      361 ccgaaatcag agaattcatt agttttaact acgtctgtat cgatgcccag tttatcaagt
      421 gtgccgctaa agttaggaaa cataccgaaa atgcctatag aacctgtgat ggtggtgggt
      481 tgagcaacaa ttttatcagc attacacgca atatagtaac cgcccgatgc agccatgtca
      541 cccatagaaa caactaccgg tttttccgac ttcagatcgg aaattgcttt ccagatttgc
      601 tccgaggcat atgcgcttcc gcctcccgaa ttgatgcgaa aaacaaccgc tttaatgtca
      661 tcgtctttcc gaagtttttc aatctcgttt acgaaatatt tatcctgaat gccattaggc
      721 ctattgccgg atgcaattcc tcctgtggcg tatagcaagg cgatggagtt tgttgatttc
      781 cgtatagccg gtgtttttac tgatttcatt tccgcaaccg ttgccgacgg aattttagtg
      841 tcagtatcta tcccgagcag ttcccggaca taatttttca tttcactttc gtagagaacg
      901 gtgtcgacta aatttcggga caacaggaaa tcggtttttc tgagcattgg aaattgattg
      961 gcgatcgaat ctatttgcat agtttccata tttctggatg cagccatatc ggttttcata
     1021 gtattccaaa tatctgtaat gaatgagttc acctgttctc tgttggcttc actcatttcg
     1081 gtttgggtaa aaggctcggc aaaagattta taagtgccca ctttaaacag ttgcatttct
     1141 acgccgagtt tttgcaacgc gtctttataa aacagcggaa ccgaagcaat tccgtgaaca
     1201 tcgagcatcc cctgaggatt gatggccacc ttatcagcaa cagaagccag ataatagccg
     1261 ttttgagtgt atgtatcggc atatgcaacg ataaacttgc cacttttttt aaaatcctcc
     1321 aactcttgcc gtatttctgc caatgtagca gtggaagctg caaaaagacg tgtatccaga
     1381 taaatacctt ttatcttgtc gttatttttt gctttacgga tggctccaat gatatcgttc
     1441 atacctatgg cagatgggac tccacctacc aggtcggtaa acgggtcact ttccgcaata
     1501 cgttcctgta tggcgccttc gagtcttaga tttaaaacgg agttattttg caatacaaat
     1561 gattcctgtt tgccaaaaga acccatgatc gagcccatca tagcaaacat gatcagcatt
     1621 gaaataagcg aaaaaataaa actcgcgatg atgaagccca atgctgatgc aaacatcatt
     1681 ttaaagaaat ctttcataaa tttgtgttct aaattgatta taagcacaaa aatagagaaa
     1741 aaagaattat attgcaattg aaaacaatta caatttagtt tctttatact tgtaagataa
     1801 tttattattg tagtgatcgg ggttttatac ctcataaatt gttttgagaa caaaagacca
     1861 cgaaagaacc tataatagtt aaaaaaatat aaagaatagg ggtgaatgga taaaccgtgc
     1921 aacgtttttt cattttttcc atctttacta atgtaagcga taagcatgga tgattcgcag
     1981 ttgataacag catgtaagaa gcagaaccgg aatgcccaaa aggcattgta tgagttgtat
     2041 gctccgaaaa tgatggctgt ctgcctgcgt tattgtcaag ataatgagac cgctcgtgat
     2101 gtgttacacg acggttttct gcaggttttt acgcaaattg gctcttattc gggcaagggt
     2161 tcttttgaag gttggttacg caagattttc gttaatctgg ctctcgaaaa ttatcgtcag
     2221 gagaaaaaaa ggttcaactt tttaaacgat tacggaaaag acaattcaga cttttgggat
     2281 actcctgagg atgactctct cgaagtagga gatattccgc gtcaagaact tctgaatatt
     2341 attcgtgaga tgcctgaagg ctacagaacg gttttcaact tagtgatttt tgaggatata
     2401 cctcataagg aaattggtgc aatgctgggg ataacagaaa acgcttcccg gtcgcaatat
     2461 ttcagagcca agactttttt acaaaagaaa gtccaatcca tgttgaagaa gaaatacata
     2521 tgatcttcta tgaacgaaaa agatatattt aaagaacaat tgaagtcggt tttcagcgat
     2581 tttgaagctc ctgttccccc tgatggctgg gagaaggtag agcagtcgct taatgccatt
     2641 aggcgggcga gaattatccg ccgaaattgg tatgtcggct ccgcagcagc tactgcggca
     2701 ttaatactgg gaagtttgtt ctttttgaat tcgcctcatc cggtcaaacc cgatctccct
     2761 gttttcaccg ggaagatggc acctgaaaaa acaggaagcc tagatacaca ggatactcaa
     2821 atagcaaaaa ctgaaacgac aaagaaggaa agtaacaagg acagtcaggt tgggtattct
     2881 cttcaaaaac aagaggtttt ggtggccagg tacaccaaaa gaaaagaaaa ggtgttgtct
     2941 gtaaacatag acttagaaca aactccggaa aagatagtga ttgctgaaca aactgttgaa
     3001 gttaaggccg aggcaatggg agaaagaata attcgtcctg aggaaaggca ggtggttcaa
     3061 cccagtcagg aagaggttga ccggctcatt caggagtttg agaatgccgg gaaagctaca
     3121 atctttgaag gtttggcgtt tgaagataag aagagtaaac ctattatgtt ggcactgaac
     3181 gcaaaaggtg gacttacttc ttcgcggaaa accgtaaata gtcccatgac tttgcgcagt
     3241 gccagtgcgg tcaagccatc tgaaaacgga gatggtcttt attccggatt aacgggatcg
     3301 aataagatgt atgctgatta cagcagtatc ccattcaaca accgaagtat tgctgataac
     3361 gtagccgaaa tggaacacga tcagccggtt tcgttcggca tcaccgtttc taaaaccatt
     3421 gttgacaggt tatccgttga aaccggattg gtttatacct atctgttttc tagggcaaaa
     3481 aacaccagtg tagattttca aaatcaggag acacaacatt ttcattattt aggtattccg
     3541 attaatctga attataactt cgtcaacata ggaaaactag gtttgtttgc atctttcgga
     3601 ggaatggtgg aaaaagatgt ctacggggaa ttcaggagta cgggacaaag tgtttcaact
     3661 gaactaaaca gtgcgtctca gggggtgatt actacgaaaa tcagtcagaa gaatccacag
     3721 atctcagtaa atgcaggggt aggggtgttt taccctattt acggaggatt taatttgtac
     3781 ggcaaggttg gtggggttta ttactttgat gcaaaaaact atgaatataa aacgatatat
     3841 accgacaaga aaatcggttt cgatttgaat gcggggatcc gatttgattt ctaatgattt
     3901 ttaatttaac aaataattat gtatatgaga aaattgattt tattttttag tgttgtgatt
     3961 gctggactct ttttgacttc atgcttagag gccggagata gtaacttttc aggtcagcgg
     4021 caattgtttt atattactca attagaggga acacaagttg cccataacgg agattttgtg
     4081 atgacttcca atgagattaa atcgaaagat ccggacaggt ggtaccttat tacctggtcg
     4141 tggagtaccg aaaacggtat ggtcagtaca aatgtacata acgctataac accggaaata
     4201 gagcaaatac ctcttggcgt atattctcaa caagaggctc ctcaagaaac catgactcct
     4261 gtccttaact ttaacatggt tggggggttg cccatagctg gttcatttgg tgatttcctg
     4321 attgttcaat atgtatggaa taagaaagaa ggcgaaaaag tacaggcgaa actctataat
     4381 gaaaccactc aaccagatgc aacggataca tatatactgg aattgcgttt agaaaaatcc
     4441 ggcacttcca cggaaacttc tggaaagaat gttgatggtt ttgcagcatt tgacgtgagt
     4501 tcattgcgta gtctggttaa ctttaacgga caagatttta aagatatcca gttaaaattc
     4561 aattattaca aagaaaatgc aggacagcca accagcgtta cacagcctgt caggatatat
     4621 aaaaactgat ttgagccgcg gtcaaaacac aatcttgact agtcctaaat aagcgttaca
     4681 ggtttataga acaatattaa ttgaatcatc agagagaaac aaggctaacc ttgtttctct
     4741 ctgatttttt atatgtaaaa gtttacactt tcttgtttat gcatctgttc aggtgttttc
     4801 acatgacaaa accaatgagg ccttttgatt gttgatgcgc acggtatctt taaccaacca
     4861 tctcattgtt tctatatcaa cttgatactc ctcgattaag aactcctatt ttaatatacc
     4921 gttcaccctt tctgcaattg cattcgcata cggatcatag ctttcggtca tacccggtgt
     4981 tatctttttg ctatttagtg cattctgatg atcgtttttt tgccaccaat ataagttata
     5041 tcggaaaccc atacctgttc tggatggttt agctgcgtac cctccactag attcttatac
     5101 tttttgaatc tatgatgaga gtcggtggtt atcctgtaat ctttttttac gtaacactat
     5161 ttcagaacaa gacatgtttc aacaataaaa aaagctaccc aaacgggcag ctttttttat
     5221 tgttgaaaca tgtgaagaag agaattttat tcttcgctct ttgtttcttc agttttattt
     5281 tcaactgttt cttctgcttc agttgcaatt ttaggttcat ccttcacttc tgcctcagca
     5341 acttttgctt cctcttcttt attagctttt gccttagctt ctttcttgaa agctgccacc
     5401 ttttggttgt cgagtttttc tttcagagca gcgagttctt cgatatcacc caacgtggtt
     5461 ttttccatta ctggaattgc cattgttgat gattggtttt ctttcttacc cgaacgtttg
     5521 ccttttctgc tttcgtcaga accgcctcct tgagcatcct cgtgaatacg gctgtgagaa
     5581 acaataatgc gtttagcatc tttattgaac tcgataactt tgaattccag tttttcgtcc
     5641 acttgagctt gtgaaccgtc ttctttcacc aggtgttttg gagtagcaaa accttctaca
     5701 ccgtaaggca atgcgattac tgcacctttg tcgacaaatt cgagaattgt cccttcgtga
     5761 accgaaccag ctgtgaaaat ggtttcaaat acatcccacg gattttcttc caattgttta
     5821 tgcccaaggc ttaaacgacg attctcttta tcaatttcca gaacctgaac tttaattgga
     5881 gctccgattt cggtaacttc gcttggatgt ttaatttttt tcgtccagga taaatcggaa
     5941 atatggatca atccttcaac accttcttca atttcaacga aggcaccaaa gttggtgaag
     6001 ttccgaacgg ttgctgtgtg ggtgcttcca acaggatatt tctcttcgat gttatcccat
     6061 ggatcgggct tgagttgttt tacgccgagc gacatttttc tctcctcgcg atcgagtgtc
     6121 aggataacgg cttctacctc gtcgcccact ttcataaagt cttgtgcgct gtgcagatgc
     6181 tgtgtccagc tcatttccga tacgtggatc aatccttcta cgcccgctgc aacttcaaca
     6241 aatgctccgt aatccgctat aactaccact tttcccttaa cgatatcgcc tactttcagg
     6301 ctgtcgtcca tggcatccca cggatgtggg gttaactgtt tcaatccaag ggcaatgcgt
     6361 tttttgtcat catcaaagtc gagaattacc acattgatct tatcgtccag tttaaccact
     6421 tcgtccggat gttggatacg tccccaggaa aggtcggtaa tgtggatcaa gccgtcaacg
     6481 ccgccgaggt caacaaatac accgtaggag gtaacatttt taaccactcc ttcgagtacc
     6541 tgtccttttt cgagtttcga aataatttcc tttttctgtt gttccagctc ttcttcaata
     6601 agggctttgt gggaaacaac tacattcttg aattcaggat tgatttttac caccttgaat
     6661 tccattgttt tgtcaacaaa gatatcgtag tcgcggatag gtttcacatc gatttgagaa
     6721 cccggcagga acgcttcgat accgaataca tccacaatca taccgccttt tgtacggcat
     6781 ttgatgtatc ctttgattac ttcgttgttt tctaatgctg cgtttacgcg atcccatgaa
     6841 cgcgaagcac gtgctttttt gtgagaaagg atcaactgtc cttttttgtc ttcctggctc
     6901 tcgatgtaaa cctctacctc gtcgccaact ttcaattcag ggttgtagcg gaattcgttc
     6961 atcgatacca cgccgtctga tttgtagccg atgtttacaa ccacttcgcg tttgttcatg
     7021 gaggttaccg taccggttac cacttcttta tcgttgattt tgctcaacgt gttgtcgtag
     7081 ctttcggtaa gtttttcttt gttttctttg gagtagacgt caccttttgc gtaagcatcc
     7141 caatcgaatt cctctatggg agctacagtt tttaaattgt cagtcatttg ttgtttgaaa
     7201 aattaataat taaataagtt agacattata ttgtcgaaaa aaaacggtgc aaagatagtc
     7261 ggttttttgt taataaccaa ttctttgcac ccggaatttt gggaaaatat caactttccg
     7321 caagtgagga caggccagca gaagatcaga taagtaatcc ttcctggact tcttttcttg
     7381 tccccgctcc ggcaatggtt tctaccaact ccaaagcaaa ctcgaaaact aatcccggcc
     7441 cttttccggt aatgatattt ttgtcaacgc tcactttgtt actgctgact ttagctccgg
     7501 tcaactgatg ttcaaaaccg gggtaacagg ttgctttttt cccttccaga agtcctaatc
     7561 cccctaatac cattggtgca gcgcaaatag cagctacaac cttgcctttt tcggcatatt
     7621 ccttcacctt tctcttaagt tcttcgtgtt cgttaaggtt ttttgctccg ggcatgccac
     7681 cgggaagtat cagtacatcc acatcgtgga aattcacctc ccggaagatt acatctgcca
     7741 gaacggttat gtgatgcgct ccggtgacgt ttttctcgtt agttatcgaa acagtctgaa
     7801 caggaatccc tgccctccgt aaaatgtcta cagttcccaa agcttcaatt tcttcgaagc
     7861 cattggctaa gaatagcgct acttttttca tctgttcatg ttttgtttac gttgtactcg
     7921 tatgataatt gaaagcggcg aaatcgccga atttaaagat gcaatcagtt gaggttaaac
     7981 ctgtacgtaa tggtcccttg ctgattgttg gcagagttga tagcattgaa tttagttctg
     8041 cgtgctgccc gcagtgcttc gttacgcagt gaagtgttcg gggtatttgt gccgcggcca
     8101 atttcggcat ttataacgtt tccgcgggga tctaccgtaa tgttgacaac gaccgttccg
     8161 taatcgttta ccgaatagtt gggttgaact aacccaccgg aacccaagct tcggcctccc
     8221 aaatcgaagg tcccgattcc acctgtaccc gatgtggctc cctgggaagc atttcccgta
     8281 gaaactcctt gggttccgct gccttcggtt tcaccccggc tgccggagtt ttcaccaaac
     8341 agtccgctca tttgctgatt tatttctctg cgcctggcat cttcttcacg ttgtctgcgt
     8401 gcagcctctt cctgtaacct acgttgttct gctaattgcg ccttacgtcg ctctgcttca
     8461 cgttgtgcag taacgtctat tgttggttct gtttgttgag tgatcagcgg ctcttcgggc
     8521 gagtattcag ggacggatgg gcgttgcgcg ggtgcaggag atatttcatt catgggagat
     8581 tccgttgtgc cggaagcctc ttccacattt ccaaacataa cggggatacc ctccagctca
     8641 tccgggggta ttaccagtgt gaagtaagag aacaaaagta caagcagaag caatacggca
     8701 aagaggattg tccctgcgat tcctgctgtt ttttctcttg taatttgcat cctgtaaaag
     8761 taattgaccg gttttctagt tcggtcttgt catcagcacc attttaaatt ggttctgatt
     8821 cgcaatatcc agtattttta ctatttcgcg gtagggcact tcctcatcgg catagagtgc
     8881 tacgaagata tccggttcag ccgagtagac cgattgcaaa aacggtgtaa tttgttcaaa
     8941 agttaccatc cgttcggtct cgttagcatt ggcaacgtaa tagttcaggc tcttgtctat
     9001 ggtaacccga gtaatgggct ttgcctgtgc ctgttgttgt gatttgggaa gcagcacctt
     9061 tattgcattt ggcactacga tggtggatgt gatcatgaag aaaatcaaca gaagaaagat
     9121 cacatcagtc atggaggcca tgctgaaatt tgcttctaca ttaaatcgtt gttttaatgc
     9181 cattttcctt ttgttttatg ccaccggttc attc
//
LOCUS       DAOSDR010000071         9211 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrimonas sp. isolate AMR_MDS_1826
            NODE_2799_length_9211_cov_3.968654, whole genome shotgun sequence.
ACCESSION   DAOSDR010000071 DAOSDR010000000
VERSION     DAOSDR010000071.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863355
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrimonas sp. (wastewater metagenome)
  ORGANISM  Petrimonas sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Dysgonomonadaceae; Petrimonas.
REFERENCE   1  (bases 1 to 9211)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9211)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:10:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,720
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 35
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9211
                     /organism="Petrimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2799_length_9211_cov_3.968654"
                     /isolate="AMR_MDS_1826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2023866"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..969
                     /gene="mraY"
                     /locus_tag="PLI30_04240"
     CDS             <1..969
                     /gene="mraY"
                     /locus_tag="PLI30_04240"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005861367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HOI78797.1"
                     /translation="YIIIMIISTIWLGALGFADDYIKVFKKDKEGLKGKIKVVAQVGL
                     GLIVGLMMYWSPDIVARENTEIRVNNVIEQVQYKDQNVKTTKTTIPFVKNNNFDYRWL
                     VSWMGDFADEAVWVVFVLSVILIVTAVSNSANLTDGLDGLASGSSAVIGVALGVLAYV
                     SGRVDFASYLNIMYIPGGDELMVFAAAFVGATVGFLWYNAYPAQVFMGDTGSLTLGGI
                     IGVFAVLIHKEMLLPILCGVFFVEALSVILQVTYFKYTKKRSGIGKRVFKMSPLHHHF
                     QKAGNAGIDALIQKPLIPITEPKIVSRFWLIGMILAVIAVATLKMR"
     gene            983..2320
                     /gene="murD"
                     /locus_tag="PLI30_04245"
     CDS             983..2320
                     /gene="murD"
                     /locus_tag="PLI30_04245"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008780721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HOI78798.1"
                     /translation="MHYELIILGGGESGVGSAILAKAKGIRVFLSDSGMLKGEHRKTL
                     VDYGIDFEEGGHTEESILSASEIVKSPGIPDTIPLMQKIISKGIRVISEIEFAGRYTH
                     AKMICITGSNGKTTTASLIYHILKSAGLNVGLGGNIGKSFARQVAEENFDYFVLELSS
                     FQLEGVYDFKADIAVLMNITPDHLDRYDHNMQNYVDAKMRIIRNHKMGDSFIYWIDDP
                     VVTKEIEKLKPASDLYSFTEKNNQRAAAYSEGNKMYIQTKGTVFDMELELLALQGMHN
                     LYNSMASGIVAKLLDISDDNLRLALSDFKGVPHRLEKVASVRGVQYINDSKATNVNSC
                     WYALQSMRTKVVLILGGTDKGNDYTEIEELVRQKARGLIFLGKDNRKLRAFFDGIVPD
                     IEEVNSMKDAVDTAYRMAEKGDTVLLSPCCASFDLFQNYEDRGNQFKDCVRRL"
     gene            2355..3704
                     /locus_tag="PLI30_04250"
     CDS             2355..3704
                     /locus_tag="PLI30_04250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019538607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HOI78799.1"
                     /translation="MLDKIKNAINPTNFQEDLENSKSTLEKFGVKIFKGDKVIWLVFV
                     ILCVISLVEIFSATSTIVYKQQNQWGPILRHTMFLIGGVGVIVLIHNIPYRYFSSLIF
                     VLIASIVLLLLTPVIGTTVNGAERWISIFGFTIQPSEIAKISLMGTIAFLLSKQNGMN
                     DGLLFKWMIGLMIVVCGIIAMDNLSTAALLFVVCFLLMFIGNVKLARLLKVGGAGIAF
                     LVLFIVLLKVIPADWTDSGALSRVGTWQNRIAEFGSHKEESEETYYTITDENYQVAHA
                     KIAIANGGVLGKFPGNSTERDFLPQAYSDFIYAIIIEEMGIAGGIFVLLLYIIILIRA
                     GMIARKTQKLFPKYLVLGSALMLSMQAFINMAVAVNLIPVTGQPLPLISRGGTSLLIT
                     CAYFGLILSADRFGIGKKKGEVTIDETDQPDERKDKKNAVETIDKLEEKVEFEIIQV"
     gene            3701..4825
                     /gene="murG"
                     /locus_tag="PLI30_04255"
     CDS             3701..4825
                     /gene="murG"
                     /locus_tag="PLI30_04255"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005866971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HOI78800.1"
                     /translation="MMSALKPLRVIISGGGTGGHIFPAISIANAIKERWSDAEILFVG
                     AENRMEMERVPAAGYTIVGLPVAGFDRKNLLKNVSVAVKLLKSMRKARAIVASFKPDI
                     AVGVGGYASGPTLRAAAAKGVPTVLQEQNSYAGVTNKMLAQKAAKICVAYEGMERFFP
                     KEKIVLTGNPTRQDLVVSEENRQKGYTYFGLNPERKTILLVGGSLGARTLNESIIGSC
                     SDIQKSDVQVIWQCGKYYFKEMSDLKAQGRIPGNVYLFDFLSRMDYAYSVADLVISRA
                     GAGSISEFSLLGKPVILVPSPNVAEDHQTQNAMALVKRNAAMMVADAEAKALLFDMAL
                     SLVKDESKLEGLSRNILKLAQRGSARRIVDEIEKIMHYEL"
     gene            4815..6191
                     /gene="murC"
                     /locus_tag="PLI30_04260"
     CDS             4815..6191
                     /gene="murC"
                     /locus_tag="PLI30_04260"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015532163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HOI78801.1"
                     /translation="MNYELIYFIGIGGIGMSNLARYFIQQGKSVAGYDRMETPLTKAL
                     VAEGAKVHYEDYVRLVPAEFLDKEKTLVVYTPAVPASHSELQFFRQSGFTIMKRAQLL
                     GEVTKTSDAVCVAGTHGKTTVSSMTAHLLRQSGVDCNAFLGGILKNYGTNLLLSDKSR
                     ITVAEADEYDRSFHWLQPWIAVITSADPDHLDIYGTPEAYRESFEHFTSLIRKNGYLI
                     LKKDAPLTPRTDATVTVFTYSESEGDFHAENIRIGNGEITFDFVSPNSRITGIQLGVP
                     VKVNIENAVAAIAAAVLSGVNPDEVRAAMKTFGGAKRRFDFQLKTPNLVFIDDYAHHP
                     QELTASIRSIRQLYPDRKVTGVFQPHLYTRTRDFADDFAQSLSLLDDVILLDIYPARE
                     EPIPGITSGVIFDRITSKEKVLLKKAELLDFLENKELDVLVTLGAGDIEHLLPAIKQL
                     LEKKSIGK"
     gene            6220..6951
                     /locus_tag="PLI30_04265"
     CDS             6220..6951
                     /locus_tag="PLI30_04265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019538604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI78802.1"
                     /translation="MKKFFIILIALLVTGYLIFSAIYFNDSSRNEVCNKFEVIVKDSL
                     HTQFIHQKDIEVLLKRKKVYPVGKTMDEINTLEILDTILTNRLVKSAEVFTTQNGTII
                     ANIYQREPVLRIISDTKGSFYIDDNRERMPVSPNSTVYVPLATGAIDEEFAQNELYDF
                     AAFLNENPEWNAWIDQIVVKPNKDVDLIPRAGDFRIAFGKLDNYPEKFARFALFIEKG
                     LNVVGWNRYSEISLKYDNQVVCTKK"
     gene            6957..8306
                     /gene="ftsA"
                     /locus_tag="PLI30_04270"
     CDS             6957..8306
                     /gene="ftsA"
                     /locus_tag="PLI30_04270"
                     /inference="COORDINATES: protein motif:HMM:TIGR01174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HOI78803.1"
                     /translation="MTQSGFIAAIDLGTSKITGIIGRKNESNVISVLECVSLPSENSI
                     RRGTIYNIDKAGAIVRKLISMLENALGRKIGKVYVSLGGQSVHTEVIREMKQLSSSGI
                     VTEEVVEQLRNAAEKYKPDMQRKYAIGDVEYFLDDKPEKNPVGVTCSMVEAEYQIVVG
                     RPNLVSNIEKTIVDKGHLQIAGYIVGPLAAAAIVLTEEEKELGCAFVDFGAGTTTLSI
                     YKGGLLRRMVTIPFGGRSITRDIAELNFVESDAEQYKIKFGKARENNESSLFSSPFSS
                     KPDIDLVELNKVIVMRLDEITANIREQIRLSGYQDQLGAGLVITGGASQLRNLEEYLS
                     QQLDLPVRKASSRKTLVNNTPEFANDPALTSALGMLLLASENCEKTVEEFEDEESSDQ
                     KSKGSVWSNFFGDRDKGDKGKEQKEKESKGKKEKSINIGGKMKNMFSTMFEEEDDDQ"
     gene            8329..>9211
                     /gene="ftsZ"
                     /locus_tag="PLI30_04275"
     CDS             8329..>9211
                     /gene="ftsZ"
                     /locus_tag="PLI30_04275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006841463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HOI78804.1"
                     /translation="MYTDILDFQINSRTEAIIKVIGVGGGGGNAVNHMFKEGIHDVSF
                     ALCNTDNQALLSSPVPVKIQLGEHTTGGLGAGNKPDVARRAAEESIDLIQDLLSDGTR
                     MAFITAGMGGGTGTGAAPVVARIAKDMGILTVGIVTIPFVFEGQRKIVQALKGVEQIA
                     ANVDALLVINNERLRDIYHDLTMLNAFARADDTLATAAKSIAEIITIHGHVNLDFADV
                     ETTLKDGGVAIMSRGIGSGKDRVNRAIEDAINSPLLNNNDVFSAKKILINLSFGEENP
                     LMMEEMNALHDFMSKFGH"
BASE COUNT         2579 a         1868 c         2395 g         2369 t
ORIGIN      
        1 tatatcatta taatgataat aagcacgatc tggttgggtg ctttaggctt tgccgatgat
       61 tatatcaagg tatttaaaaa agacaaggaa gggctgaaag gaaaaatcaa agtcgtggca
      121 caagtcggtt tgggtttgat tgtaggcttg atgatgtact ggagccccga tatcgttgcg
      181 cgtgagaaca ccgaaatccg ggtgaataat gtcattgaac aggtgcagta taaagatcag
      241 aatgtcaaga ccacaaaaac gaccatccct tttgtgaaaa acaataattt tgattatcgc
      301 tggctcgttt cgtggatggg cgattttgcc gatgaagcgg tctgggttgt ctttgtgctt
      361 tctgtaattc tgattgttac ggcagtttca aacagtgcga acttgacgga cggactggat
      421 ggcctggcct ccggatcgtc agcggtcatc ggtgtggcgc tgggagtcct ggcatacgtg
      481 tccgggcgtg tcgactttgc ttcatacctg aacatcatgt acatccccgg aggggatgaa
      541 ctgatggttt ttgccgctgc atttgtgggg gctactgtcg gttttctctg gtacaacgcc
      601 tatcccgccc aggtttttat gggcgatacg ggtagcctga cgttaggcgg gattatcggg
      661 gtttttgccg tgcttatcca taaggagatg ctgcttccta tcttgtgcgg ggtgtttttt
      721 gtagaagccc tctctgtaat tctgcaggtc acttacttta aatatacaaa aaaaagatcg
      781 ggtatcggga aacgggtgtt taaaatgtcg cccctgcatc accattttca gaaagcagga
      841 aatgccggga tcgatgccct gattcagaaa ccgctgatcc cgatcaccga acccaagatc
      901 gtttcgcgat tctggcttat aggaatgatt ctggcggtaa tagccgtagc aacgttgaag
      961 atgaggtagt aaatcatata ttatgcatta tgaattaata atattgggtg gtggcgaatc
     1021 gggagtaggt tctgccattc ttgctaaggc gaaaggaatc cgggttttcc tttctgattc
     1081 tggaatgctt aagggggaac accggaaaac gcttgtcgac tacggaattg attttgaaga
     1141 aggcggccac acagaggaga gcatcctctc tgcgagcgag atagtaaaga gcccgggcat
     1201 tcccgatacc atacccttga tgcagaaaat tatttcaaaa ggaatccggg tgatatccga
     1261 aattgagttt gccggaaggt atacccacgc caagatgatc tgtattaccg ggagcaacgg
     1321 taaaaccact acggcaagcc ttatttatca catcctgaaa tcggcaggac taaacgtggg
     1381 gctgggagga aacataggca aaagctttgc caggcaggtg gcggaagaga atttcgacta
     1441 ctttgtgctg gagttaagta gttttcagct ggaaggcgtg tatgatttta aagcggatat
     1501 cgccgtattg atgaacataa ctcccgacca cctagatagg tacgatcaca atatgcaaaa
     1561 ttacgtggat gcaaaaatgc gaatcatacg caaccataaa atgggagatt cttttattta
     1621 ctggatagac gatccggtag tgacaaagga aatcgaaaaa cttaaacccg catctgattt
     1681 atattctttt actgaaaaaa ataatcaacg ggctgcggct tattcggagg gcaataaaat
     1741 gtatatacaa accaaaggaa ctgtttttga tatggagttg gaattgctgg cgttacaggg
     1801 aatgcataat ttgtataact ctatggcgtc ggggattgtg gctaaattac ttgatatctc
     1861 ggacgataat ttacgcctgg ctctttctga ctttaaaggc gttccgcacc gcttggaaaa
     1921 agtagcgtcg gtgcgaggtg tgcaatacat aaatgactcc aaggctacca acgtaaactc
     1981 gtgctggtac gcacttcaga gtatgcgcac gaaagttgtg ctgattcttg gcggtaccga
     2041 taagggaaac gattacaccg agattgaaga gctggtaagg caaaaggcta gggggttgat
     2101 attcctggga aaagataacc gcaagctgcg tgctttcttc gacgggatcg tccccgacat
     2161 agaagaggta aactccatga aagatgctgt tgataccgct taccggatgg cagaaaaagg
     2221 ggataccgta ttgctgtcgc cctgttgcgc cagcttcgac ctgttccaga attacgagga
     2281 ccgtggtaat caattcaagg attgtgtgag gaggttgtaa gggtggggtt tgagataact
     2341 aaaaaaatga aaatatgctt gataaaataa agaatgccat taatccgaca aattttcagg
     2401 aagatttaga aaactcaaaa tccacgttgg agaaattcgg agtgaaaatc tttaaaggtg
     2461 ataaagtcat atggttggta tttgttatct tgtgtgtgat atcactggtg gagatcttca
     2521 gtgctaccag caccattgtt tataaacagc aaaatcaatg gggccccatc ttacgccaca
     2581 ccatgttttt gatcggagga gtgggggtca ttgttttgat tcacaatata ccttaccggt
     2641 atttctcttc cctgattttt gtgcttatcg cttcgatcgt cttactcctg ctcacaccgg
     2701 ttatcgggac tacggtaaac ggtgctgagc ggtggatctc tatctttggt tttaccattc
     2761 aaccgtcgga aatagccaag atatcgctta tgggcacgat agcgtttctg ctcagtaaac
     2821 aaaacggcat gaacgacggc ttgctcttca agtggatgat cgggttgatg attgttgttt
     2881 gcggcattat tgcaatggat aatttgtcga cggcagcgct gctctttgta gtttgttttt
     2941 tgctgatgtt tatcggaaat gtaaagttgg caagattgct aaaagtgggc ggagcaggga
     3001 tagcctttct cgtactcttc attgtgttgc tgaaagtgat tcccgccgat tggaccgatt
     3061 ccggcgcgtt aagccgtgta ggtacctggc aaaaccggat tgcagaattt ggcagccaca
     3121 aggaagaatc ggaagagaca tactacacca ttaccgacga gaactatcag gtggcacatg
     3181 ctaaaatagc cattgccaac ggaggagttt taggtaagtt cccgggcaac agtacagagc
     3241 gtgattttct tccccaggct tattccgatt ttatttatgc gataatcatc gaggagatgg
     3301 gtattgccgg tggaattttt gtgctgctgc tctacatcat catcctgatc cgggcgggaa
     3361 tgatcgcccg gaaaacccag aaactttttc cgaaatatct tgttctgggc tcggcactca
     3421 tgttatcgat gcaggcgttt attaacatgg ctgtggctgt gaatttgatc ccggtaaccg
     3481 gccagccgtt gccgctcatc agccggggag ggacgtcact gctcatcact tgcgcttact
     3541 tcgggttgat tttaagtgca gacaggtttg gaatcggaaa gaagaaagga gaggtgacca
     3601 tcgatgaaac agatcagcct gatgagagaa aagataaaaa gaacgctgtg gaaaccatag
     3661 ataaattgga agaaaaagtg gaatttgaaa ttattcaggt atgatgtcag cattaaagcc
     3721 cctacgggta atcattagcg gcggtggtac cggcggacat atttttcccg ctatctccat
     3781 cgctaacgct ataaaggaac ggtggagcga cgccgaaatc cttttcgtcg gtgcggaaaa
     3841 ccggatggaa atggagcgtg ttccagctgc aggatacacc attgtcggac ttcccgtagc
     3901 cggtttcgac cggaaaaatc tgctgaaaaa tgtttcggta gccgtgaaat tattgaagag
     3961 tatgcgaaag gctagagcca ttgttgcctc gttcaaaccc gatatcgctg tgggagtagg
     4021 cggctatgcc agcgggccca ccctccgggc ggcggctgca aaaggcgttc cgacagtttt
     4081 gcaggaacag aattcatatg ccggagtaac caataagatg ctggcgcaga aagcggccaa
     4141 aatctgtgtg gcgtacgaag gtatggaacg gttctttccg aaagaaaaaa ttgtgctcac
     4201 cggcaatcct acgcgacagg atttagtcgt ttccgaggaa aacaggcaga aaggatatac
     4261 gtacttcggc ttaaaccctg aaaggaaaac aatactcctg gtgggtggca gtctgggtgc
     4321 gcgaacgctt aacgaaagca tcatcggatc gtgctccgat atacagaaat ccgatgttca
     4381 agttatctgg caatgtggaa agtactattt taaagaaatg tctgacttga aggcgcaggg
     4441 aagaattccc ggaaatgtgt atctgttcga tttcctctcg cgcatggatt atgcctactc
     4501 cgttgccgac cttgtgattt cacgtgccgg agccggttcc atatcggaat tctctctgct
     4561 gggaaaaccg gttatccttg tcccgtcacc taacgttgcg gaggatcacc agacacagaa
     4621 cgccatggcg ttggtgaaaa gaaatgcggc tatgatggtg gccgacgccg aagcaaaggc
     4681 actcttgttt gatatggctt tgagtctggt aaaagatgaa agcaaattag aggggttgag
     4741 ccggaatatc ttgaaattgg cgcaacgagg ttcggcccgg cgtatcgtgg atgagataga
     4801 aaaaattatg cattatgaat tatgaattaa tatactttat cggtatcggc ggtatcggca
     4861 tgagtaacct tgcccggtat tttatacagc agggaaaatc ggttgccgga tacgatcgca
     4921 tggaaacccc gcttaccaaa gcgctggtcg ccgaaggagc gaaagtccat tatgaggatt
     4981 atgttcggct cgtacccgct gaatttctcg ataaagaaaa gacacttgta gtctatactc
     5041 ccgcagtacc ggcatcgcac agtgaattgc agtttttccg gcaaagcggt tttacgataa
     5101 tgaaacgtgc ccaactcctt ggtgaggtaa ccaagacgag cgacgcagtt tgtgtggcag
     5161 ggacacacgg gaagacgacg gtgtcaagca tgacagctca cctgttgcgg caatcgggag
     5221 tagattgcaa cgcatttctg ggtggaatcc ttaaaaatta cggcaccaac ctgctgctgt
     5281 cggataaaag tcgcattact gttgccgaag cagatgagta tgaccgttct tttcattggc
     5341 tgcaaccctg gattgcggtt attacctcgg ccgatcccga ccacctggat atttacggga
     5401 cgccggaggc ctaccgcgaa agttttgaac atttcacatc acttatccgg aagaacggat
     5461 acctgatcct aaagaaagat gcaccgctaa ctccaagaac ggatgcaacg gtgacagtct
     5521 ttacctattc cgaatcagaa ggcgattttc atgctgaaaa tatccgcatc ggcaacgggg
     5581 aaattacgtt cgactttgtg tcgcccaata gccggattac cggtatacag ctgggtgtgc
     5641 ctgtgaaggt gaacatcgaa aacgcggtag ctgcaatagc tgcagctgta ttgagcggtg
     5701 ttaatcccga tgaggtacgt gcggcaatga aaactttcgg gggggcgaag cgccggttcg
     5761 attttcaatt gaaaactccc aacctcgttt ttatcgatga ttatgcgcac catccacaag
     5821 agttgacagc atccatccgc tctatcaggc aattgtatcc cgaccggaaa gttaccggtg
     5881 tttttcaacc ccacctttat acgcgtacgc gcgactttgc cgatgatttt gctcaaagcc
     5941 tctcgttgct cgacgatgtg atcctgctgg atatttatcc ggcacgggaa gaacccatac
     6001 cgggaatcac ttcgggggtt atctttgata gaatcacatc gaaggaaaag gtgcttttga
     6061 aaaaagccga actgctcgat ttcttggaaa acaaagagtt ggacgttttg gttacattgg
     6121 gtgcaggaga tatagagcac ttattgcccg cgattaaaca attgttagaa aaaaaaagta
     6181 tcggaaaata aatccgatac aatttgtatc tttgcgtaaa tgaaaaagtt tttcatcata
     6241 ttgattgcgt tgttggtaac cggttatctt attttttcgg ctatttactt caatgattca
     6301 tccagaaacg aggtgtgcaa taaatttgaa gtcattgtaa aagacagctt gcacactcag
     6361 ttcatccatc aaaaagacat cgaggttttg ctgaaaagaa aaaaagttta tccggtaggt
     6421 aaaactatgg acgaaatcaa tacgctggaa attttggata cgatactgac caaccggttg
     6481 gtaaaatcgg cggaagtgtt taccacgcaa aatggaacaa ttattgcgaa catttatcaa
     6541 cgcgaaccgg ttctgaggat tatctccgac acaaagggga gcttttatat cgatgataat
     6601 cgggaacgga tgcctgtctc gccaaattct acggtgtatg ttcccttggc taccggagcc
     6661 attgatgaag aatttgccca gaacgagctt tacgactttg ctgcgtttct gaacgaaaat
     6721 cccgaatgga acgcatggat cgatcaaatt gtggttaagc ccaacaaaga cgtggatttg
     6781 attcctcgtg ccggagattt caggatcgcc tttgggaaat tagataacta tcccgaaaag
     6841 tttgcccggt ttgccttgtt catcgagaaa gggttgaatg tggtaggttg gaacaggtat
     6901 tcagaaataa gtttgaaata cgataaccaa gtggtttgta caaagaaata aaatatatga
     6961 ctcaatcagg atttatagct gccatcgatt taggaacttc caaaattacc ggcattatcg
     7021 ggcgtaaaaa cgaaagcaac gttatttccg ttttggagtg tgtgtcttta ccgtcggaga
     7081 acagtatccg tcgggggaca atttataaca ttgataaagc cggggcaatc gtaaggaaac
     7141 tgataagcat gctcgagaat gcgttgggaa ggaaaatcgg gaaagtgtat gtctccctgg
     7201 gaggacaatc ggtgcacacg gaagtaatcc gggagatgaa gcaactctct tcttccggca
     7261 tcgttaccga agaggtggtg gaacaactcc ggaatgcggc cgaaaagtac aagcccgata
     7321 tgcagcgaaa atacgctatt ggggatgtgg agtatttttt ggacgacaag ccggaaaaaa
     7381 atccggttgg ggtaacctgc tccatggtag aggcagaata ccagattgtg gtgggccgtc
     7441 ccaacctggt atcgaacatc gagaaaacca tcgttgataa aggacacctg caaattgccg
     7501 gatacatcgt tggcccgctt gctgccgcag ctatagttct taccgaggag gaaaaagagt
     7561 tggggtgcgc atttgtcgat tttggtgccg gaacgactac gttatccata tataaaggag
     7621 gccttttgcg ccgtatggtg accataccct ttggcgggag aagcataacc cgggatattg
     7681 cggaactcaa ttttgtggaa agcgatgcgg aacaatacaa gataaaattc ggcaaagccc
     7741 gggaaaataa tgaaagttcg cttttctctt cacccttttc atcaaaaccc gatatcgacc
     7801 tggtagagtt gaacaaggtt attgtgatgc gtcttgatga gattacagcc aacatcaggg
     7861 aacagatccg gctttccgga tatcaggacc agctgggtgc agggcttgtt atcacaggtg
     7921 gtgcgtcgca actgagaaat ctggaagagt atttgagtca gcagctcgat ttacctgtcc
     7981 gcaaggcatc ttcacggaag accctggtaa ataacacgcc ggagtttgcc aatgatcctg
     8041 ccctaacttc agcgctggga atgcttttgc ttgctagtga aaattgcgag aaaacggttg
     8101 aagaattcga agatgaggaa tcctcggacc aaaagtcgaa aggatcggtt tggagtaatt
     8161 tctttggaga ccgggacaag ggagacaaag gcaaagagca aaaagagaag gaaagcaagg
     8221 gtaaaaaaga gaaatccatc aacataggcg gaaaaatgaa aaatatgttt tctactatgt
     8281 ttgaggaaga agacgatgat cagtgaatga aaaaaaatat ttccaacaat gtataccgat
     8341 atactagatt ttcaaataaa cagccgtacc gaagcgatta ttaaagttat tggggtgggt
     8401 ggtggtggcg gaaatgcagt aaaccacatg tttaaagaag ggatacacga tgtttccttc
     8461 gcactttgca ataccgataa ccaagccttg ttgagttcac ctgtgcctgt gaaaatacag
     8521 ctgggcgaac ataccacagg agggttgggt gcagggaaca aacccgatgt ggcccgacgg
     8581 gctgcagaag agagtatcga cctgattcag gatttgctga gtgacggtac ccgtatggcg
     8641 ttcattacag ctggaatggg cggaggaacc ggaaccgggg ctgctcccgt tgtggcacgc
     8701 atagctaagg atatgggcat tctcacagtg ggaatcgtta ccattccttt cgtttttgaa
     8761 ggacagcgta agattgtaca agcattgaaa ggtgtagaac agattgctgc caatgtggat
     8821 gcgttgcttg tgatcaacaa cgagcgttta cgggatatct atcacgacct gacgatgctg
     8881 aatgcttttg cccgggccga tgatacgctg gcaaccgccg ccaagagcat tgcagagatc
     8941 atcactatac atggacacgt aaatcttgac tttgccgacg tggaaaccac tttgaaagac
     9001 ggtggtgtag ctattatgag tcgcggcatt ggttcgggaa aggatcgtgt gaacagggct
     9061 atcgaagatg ccataaattc tccgttgctc aataacaacg atgttttcag tgcaaagaag
     9121 atcttgatca acctctcttt tggcgaggaa aatccgctta tgatggaaga gatgaacgcc
     9181 ctgcacgact ttatgtcgaa attcggccac g
//
LOCUS       DAOSDR010000072         9209 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrimonas sp. isolate AMR_MDS_1826
            NODE_2801_length_9209_cov_4.822919, whole genome shotgun sequence.
ACCESSION   DAOSDR010000072 DAOSDR010000000
VERSION     DAOSDR010000072.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863355
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrimonas sp. (wastewater metagenome)
  ORGANISM  Petrimonas sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Dysgonomonadaceae; Petrimonas.
REFERENCE   1  (bases 1 to 9209)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9209)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:10:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,720
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 35
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9209
                     /organism="Petrimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2801_length_9209_cov_4.822919"
                     /isolate="AMR_MDS_1826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2023866"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            223..696
                     /locus_tag="PLI30_04280"
     CDS             223..696
                     /locus_tag="PLI30_04280"
                     /inference="COORDINATES: protein motif:HMM:NF025426.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4252 domain-containing protein"
                     /protein_id="HOI78805.1"
                     /translation="MYKRLSLILLCGLLAFSAYSQSKVSFEHLYNRFAQEKNAEKVKV
                     GGLLMFLTKPLVAKYADGLNISSVHVLALDECSQDVKLRFNTLAEKLNDDKYEVLLKA
                     NDKEEKTRILARFQNDAIRELVIVSMGNEPTLVRIKGKIKPEDVQKLVNPDNNEQ"
     gene            686..1192
                     /locus_tag="PLI30_04285"
     CDS             686..1192
                     /locus_tag="PLI30_04285"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOI78806.1"
                     /translation="MSNEEFKKRFLSFHSLIYRISCRILENGDDADDITQEVYIKLWE
                     QRNNLGNIRNDEAFVVTLTKNLSIDWLRKNHRKTTSVVDNKDIRCENREEERMDARDE
                     LSNLMKCMSTLPKTQQEVIRLRHFADLSIREIAEATRQTETNIRQLLSRARRTMKEKL
                     NRNGNEQQ"
     gene            1179..1700
                     /locus_tag="PLI30_04290"
     CDS             1179..1700
                     /locus_tag="PLI30_04290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI78807.1"
                     /translation="MNSNDIRKLVEKFYEGKTSLEEERTLADFLLRDNVPDEFFPDSK
                     LFYALNTHSMEVTNESIQAIESLIDSFKEEQPSDKKIKMSHAKYWAIGVAASFALILG
                     VRQFQKSQQAESTLFTDTYKNPDDAYRATVEALQLFSDNFSKGTESVEKANMHLEKAQ
                     EIINQSLKQGIEK"
     gene            1697..2173
                     /locus_tag="PLI30_04295"
     CDS             1697..2173
                     /locus_tag="PLI30_04295"
                     /inference="COORDINATES: protein motif:HMM:NF025426.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4252 domain-containing protein"
                     /protein_id="HOI78808.1"
                     /translation="MKKTILSILLLAGFTFTFAQKNPFEQFSEMDGVTSVYISKTMLS
                     MIPKNSKMNYGGVNVGSFLNKLSSILILTSEDQKIAPQMLSMANNRVKGRDYELLMRV
                     KSDDNDNVNFFMKGKPEDIRELIMIVAGNDGENVVMQFLGNFTLDDIQKMTEGFNK"
     gene            2268..3908
                     /locus_tag="PLI30_04300"
     CDS             2268..3908
                     /locus_tag="PLI30_04300"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HOI78809.1"
                     /translation="MKFYGISLFLFFSIALSAQNTQLQYRFRVYLKDKGNPVFPVSEP
                     EKFLTPKAIERKKQQQVKIDQSDLPISPDYFNQLKNAGGKPVSYSKWFKTIVIQLRDS
                     SQINAILQLPFVDSARYVWRGIERNYRNPVRPRLGWPDCPEESGEENGFGLTEKQFAL
                     HNAQQMVLSGFTGKGINVAVIDAGFTNADVIPQFGDVNLAGFRSFVPEGDIFASSDHG
                     TKVFSTMAANLPGVMVGSAPSARYYLLRSEDVVSEFPVEEDYWVRAVEYADSIGIDLI
                     NSSLGYTAFDDTTLNYKPESLDGKTSFMTLAANRAYEKGMILVTSAGNEGNKPWQKIS
                     APGDAQHALTVGAVGTDSLIALFSSKGFTADNRLKPDIVSAGKNTVTISYNGLLDFTN
                     GTSLSSPFVAGLVASLWSVNPSMNRAEVLDIVKRSSDRYNRPDSVYGYGIPDFRKAVR
                     VVLSKLETHEKLVAEDCFSISRTAKNSYEITITEPDFSFDAYTVNVLDESGNLIAKHE
                     FENEKLTVPVQQEVKKANQFIHFVFKSPFTQKTVRFKL"
     gene            3943..5184
                     /gene="xseA"
                     /locus_tag="PLI30_04305"
     CDS             3943..5184
                     /gene="xseA"
                     /locus_tag="PLI30_04305"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007846970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HOI78810.1"
                     /translation="MPENSLSLSELNGQVSDAIRDHLPDTYWVRAETSDVRLNRNGHC
                     YLEFIEKDARGQNIVARARGVIWNNVYEMLSSYFETETGQPFVSGLSILVRVSVDFHE
                     LYGFSLSVVDIDPSFTIGEIARNRQLVLRKLEEEGVLTLNKELTFPELTNRIAVISSP
                     TAAGYEDFCDQLAKNPYGFVFYTRLFPAIMQGERSEPSVIAALEKIYAHKEHFDAVTI
                     IRGGGSSSDLSCFDSYYLATNCAQFPLPVISGIGHERDITVIDIVAHTRAKTPTAVAE
                     LLISHASKTAVELMDLQQRLVEKSGLILQSLQDKLGELSRRMIHSSRFFVREELSVVQ
                     QLSIILNHQAKRLLQAENHSVAEKSQYIHMASPENILKRGYTLTLKNGKIIKSATELS
                     EDDIIETLFTDGKAESIITKK"
     gene            5192..5731
                     /locus_tag="PLI30_04310"
     CDS             5192..5731
                     /locus_tag="PLI30_04310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI78811.1"
                     /translation="MTKKDFFRIVLRILGLYFLAMVVFNYLPSIVVFFHSLPLLSLIL
                     AIVVVSLFAYIFLLLILKPDPVIRIFKLDKGFDDDALSAKRIEFVNLLKIAIIATGLF
                     LIISHLPTFLTHLLFLLKLVVKNRNEITDHVESALLTDYISWGIKILSLIVGYLMITN
                     YSAIARFIIKKDSEKQSDK"
     gene            5728..5925
                     /gene="xseB"
                     /locus_tag="PLI30_04315"
     CDS             5728..5925
                     /gene="xseB"
                     /locus_tag="PLI30_04315"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006847759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HOI78812.1"
                     /translation="MKESALTYSQAKQELEEIVSAIESGELDVDALTEKVKRASELIA
                     FCKERLTKTDEELQKILDEIN"
     gene            5932..6621
                     /locus_tag="PLI30_04320"
     CDS             5932..6621
                     /locus_tag="PLI30_04320"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006841840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HOI78813.1"
                     /translation="MIPEQHFSVVIVAGGKGLRAGGEIPKQFQPIGGKPILMRTAEAF
                     YNFDYRMRIVIVLPEEFRFFWEELCEKHSFRIPHSIVSGGETRFHSVKNGLEEIQKDE
                     IVGIHDAARPFIPKEVIGRCYREAFDFRCGVIPVIEEKNSVRILTAYESKALDRKKIR
                     VVQTPQVFPADLLKNAYETPYREEFTDDASVAEDSGIQIKLVKGDERNIKITTPLDLK
                     FADYLCRNNLV"
     gene            6763..7236
                     /locus_tag="PLI30_04325"
     CDS             6763..7236
                     /locus_tag="PLI30_04325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019538472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC ligand binding domain-containing
                     protein"
                     /protein_id="HOI78814.1"
                     /translation="MEKIDKLDRQILEIISQNARIPFRDVADQCGVSRAAIHQRVQRM
                     VENNVITGSGYHVNPKILGYAASAYIGVKLEKGSMYKNVIPEFEKIPEVTECHFTTGP
                     YTLLIKLYAKDNEHLMDLLNNKIQEIPGVVATETMISLSQSVKREIPILKESPRE"
     gene            7371..7958
                     /locus_tag="PLI30_04330"
     CDS             7371..7958
                     /locus_tag="PLI30_04330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019538475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI78815.1"
                     /translation="MEDKQLNEKESLELITQMIRNTQKKMEKGSGIPFLIWGYVTIAV
                     SLTVWYLLGKTGNQNWNFLWFAIPVIGFPVMLLALKRKTTLPKTYIDKVISYVWIVMG
                     VSALIPSMAAAVIHNFPVLFLVVLLISAGTAMTGLVIKFKPLVISGFGGMLLSILCLI
                     LRESLDSILVFAALFLLVQVIPGHILNYKNRKNHV"
     gene            7951..8235
                     /locus_tag="PLI30_04335"
     CDS             7951..8235
                     /locus_tag="PLI30_04335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009275783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HOI78816.1"
                     /translation="MFKELDPLLHSELRLAVMSILLSVDEADFVYLKEQTKATSGNLS
                     VQIDKLNEAGYIEVERGFAGKRTRTVCKITPVGVKAFEDYVESLKSYLKL"
     gene            complement(8481..8849)
                     /locus_tag="PLI30_04340"
     CDS             complement(8481..8849)
                     /locus_tag="PLI30_04340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI78817.1"
                     /translation="MEDYLDRINKIVELENITVSKLESIIGASKNVLHKPMKNKTDIQ
                     TKWLIKISENFPQYSLDWLLIGRGEMYRNQSEDSSMAEDPVNGFKIAIKEDRLLKIIE
                     SQQRTIENLSKGGGENAGSA"
     gene            8985..9197
                     /locus_tag="PLI30_04345"
     CDS             8985..9197
                     /locus_tag="PLI30_04345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HOI78818.1"
                     /translation="MTFTDHANSLSNERVEFIKRIAEITLASPATVSRWISGEFIPSK
                     VRRTIISKELNIPEEQLFPQKENETV"
BASE COUNT         2822 a         1915 c         1954 g         2518 t
ORIGIN      
        1 tatcccaact acaaacagtc gggtaacctc aacgagttct ggatgattat tggtgtatgt
       61 ctcattttgg cagttttact ccatttcgtt ttaaaacgaa gagaaaaaaa ccgggagaaa
      121 ttcaacaagt aattcttttt tctgtcacaa atctaatccc ttttcgtctt ctttttaaaa
      181 ggaagacaaa tcttattttg caatcaccaa aaattctatt ttatgtacaa acgtctatca
      241 ctcatattgc tgtgcggatt attggcattc tccgcctata gccagtcgaa agtatcgttc
      301 gaacacctgt acaacaggtt tgcgcaggag aagaacgcag aaaaagttaa ggtgggcgga
      361 ttgctgatgt ttctgaccaa gccccttgta gcgaaatacg ctgacggttt aaatatttcg
      421 agcgttcatg ttctcgctct ggatgaatgt tcgcaggatg taaaactcag gttcaatacg
      481 ttggctgaaa aactgaatga tgacaaatat gaggttctgc tgaaagcaaa cgataaagaa
      541 gaaaaaacaa gaatactcgc acgatttcaa aacgacgcaa tccgggaact ggtcatcgtt
      601 tccatgggta atgaaccgac tctcgtacgc atcaaaggaa aaattaaacc cgaagatgta
      661 caaaaattag tcaacccgga caacaatgag caatgaggaa tttaaaaagc gttttttatc
      721 ctttcattcg ttgatttatc gaatttcgtg ccgtattctc gaaaacggag atgacgctga
      781 tgatattacg caggaagtgt atataaaact ttgggaacag aggaataatc ttggaaacat
      841 ccgtaacgat gaagcctttg tggtaaccct gacaaaaaac ctgtccattg actggctcag
      901 gaaaaatcac cgcaaaacga catccgttgt tgacaacaaa gacataaggt gtgaaaacag
      961 ggaagaggaa agaatggatg cccgggacga attatccaat ttgatgaaat gtatgagcac
     1021 tttacccaaa acacaacagg aagtaatcag gctcaggcac ttcgctgatc tgtcgatcag
     1081 agagatagcc gaagccactc gtcaaacgga aaccaatatc cggcaactat tatcgagagc
     1141 cagacgtaca atgaaagaaa aattaaacag aaacggaaat gaacagcaat gatatcagaa
     1201 aattagtaga aaaattctac gaaggaaaaa cttcgttgga agaggagaga acactggctg
     1261 attttcttct acgggataac gtgccggacg agtttttccc agacagcaaa ctgttttatg
     1321 cattgaacac gcattcaatg gaggtcacca atgaatcaat ccaggcaata gaatctctca
     1381 tcgactcgtt taaagaggaa caaccgtcgg ataaaaaaat aaagatgtcc cacgcaaagt
     1441 attgggccat tggcgttgca gcatcgttcg cgcttatttt aggagtaaga cagtttcaaa
     1501 aaagtcagca ggcagagtct acacttttta ccgacactta caaaaatcct gacgatgctt
     1561 atcgggctac cgtagaggcc ttacaactct tttcggataa tttctccaag ggaaccgaat
     1621 cggttgaaaa agccaacatg catctggaaa aagcacaaga gataatcaat caatcactta
     1681 aacaaggcat agaaaaatga aaaaaacaat cttatccata ctgttactgg caggtttcac
     1741 atttactttt gcacaaaaaa acccgtttga acagttctcc gaaatggacg gtgtaacatc
     1801 ggtttacata tcaaagacga tgttgtccat gattcccaaa aattctaaaa tgaattacgg
     1861 aggagtaaac gtgggaagct ttttaaacaa attgtcatcg atcctcatcc ttacatccga
     1921 ggatcagaaa attgccccgc aaatgctttc catggctaac aaccgcgtga aaggacgtga
     1981 ttacgaactg ttgatgcgcg tgaagtcgga cgataacgat aacgtcaatt tctttatgaa
     2041 aggtaagccc gaggatatcc gtgagctgat tatgattgtt gccggaaatg acggtgaaaa
     2101 cgtggtgatg cagtttttgg gaaatttcac actcgacgat attcagaaaa tgacagaagg
     2161 gttcaataaa taaccgtccc ttccgacaag atcaaatccg ttaagcaagg taattgcttg
     2221 acggattttt tgcgtatatt tgtccctcca tcatgtatcg agataaaatg aaattctacg
     2281 gcatctcact tttcttgttt ttcagcattg ccttgtccgc tcaaaacacg cagttgcagt
     2341 atcgattcag ggtttacctg aaagataagg gaaatccggt attcccggtt tccgagcctg
     2401 aaaagttcct caccccgaaa gctattgagc gtaaaaagca acaacaggta aaaatagatc
     2461 aatcggacct ccctatttct cccgactatt tcaatcaatt gaaaaatgcg ggcgggaagc
     2521 cggtttcgta cagcaaatgg tttaaaacca tcgtgattca attaagagac agctctcaga
     2581 tcaacgctat tctacaactc ccgtttgtgg attctgccag atatgtttgg cggggtatcg
     2641 aacggaatta ccgcaaccct gttcgccccc gactcgggtg gcccgattgc ccggaagaaa
     2701 gcggagagga aaatggattc ggacttacag aaaaacaatt tgcgctccac aatgcccaac
     2761 agatggtgtt gagtggattc accggaaaag gcataaacgt agccgttatc gatgccggat
     2821 ttaccaacgc cgatgttatc ccgcaattcg gggatgttaa cttggcaggg ttcaggagtt
     2881 ttgttcccga aggcgatatt tttgcatcta gcgaccacgg caccaaagtc ttttcgacca
     2941 tggcggcaaa cctgcccggg gtaatggtgg gatcggctcc ttctgcgagg tattatttgc
     3001 ttcgttccga agacgttgtg agtgagtttc ccgtggaaga ggattactgg gtacgggccg
     3061 ttgaatatgc cgacagcatc gggatcgacc tgataaattc atcgctggga tacaccgcct
     3121 tcgacgatac taccctgaat tataaacccg aaagtttaga cggaaagaca tcttttatga
     3181 ccctcgctgc caatagagct tacgaaaagg gaatgatcct ggtcacaagt gcggggaatg
     3241 aaggcaataa accgtggcag aaaatatcgg ctccaggcga tgcgcaacat gccctgaccg
     3301 tgggagcagt tggaacagac agcctaattg ctttgttcag ttcaaaaggt tttacggccg
     3361 acaaccgctt gaaacctgat attgtttccg caggaaaaaa tacggtaact ataagttaca
     3421 acggcttatt agatttcacg aacggaacat cgctttcatc gccttttgta gccgggcttg
     3481 tggcttcgtt atggtcggta aatcctagca tgaaccgggc ggaagtgctg gatattgtga
     3541 aacgatcgtc cgaccggtat aaccgtcccg attctgttta cgggtacggt atccccgatt
     3601 tcaggaaagc ggtaagggtc gttttaagca aactggaaac ccacgaaaaa ctggttgccg
     3661 aagactgttt ttccatatcg cgtactgcga aaaacagtta cgagataacc attaccgagc
     3721 ccgatttttc attcgatgcc tacacggtaa atgtgttgga tgaatcgggt aacctgatag
     3781 caaaacacga gtttgaaaac gaaaagctca ctgttcctgt tcaacaggag gtcaaaaaag
     3841 ctaatcaatt catacatttc gtcttcaaat caccttttac acagaaaacg gtccggttca
     3901 aattgtgatc cgggactgat aaaacaaacc aaaaataacg gtatgcccga aaattcactt
     3961 tcgttatcgg aactcaacgg tcaggtgagt gacgccatcc gcgaccacct gcccgacacc
     4021 tattgggtac gtgccgaaac cagtgatgtg cgcctcaacc ggaacgggca ttgttattta
     4081 gagtttattg aaaaagatgc cagggggcaa aacattgttg cgcgcgcgcg tggagttatt
     4141 tggaacaatg tgtatgaaat gctttcatcc tatttcgaga cggagacagg acagccattt
     4201 gtatcgggat taagtatttt ggtacgggtc tcggtggatt ttcacgaact gtacggcttc
     4261 tctctctcgg tggtggatat agatccttcg ttcactatcg gcgaaattgc ccgaaaccgg
     4321 caactcgttc tcaggaagtt ggaggaagaa ggcgtcttaa cgcttaacaa agagcttacg
     4381 tttcccgaac tgacgaaccg gattgcggta atttcatcgc caacagccgc cgggtacgaa
     4441 gatttctgcg atcaattggc aaaaaacccg tacgggtttg tgttttatac ccggttgttt
     4501 ccggcaatca tgcaagggga gcggagtgaa ccgtcggtaa tcgctgccct ggaaaaaatt
     4561 tacgcccaca aagaacactt cgatgccgtt accattattc gcggcggcgg ttcttcgtcc
     4621 gatttaagtt gttttgattc ttactatctg gcaaccaact gtgcgcagtt cccgcttcct
     4681 gtaatcagcg gaatcggcca cgaacgagac atcaccgtga ttgatatcgt tgctcacaca
     4741 cgggcaaaaa ctccgacagc agtagccgaa ctcctgattt ctcacgcctc caaaacggcc
     4801 gtcgagttga tggatttaca gcagcgtctg gtagaaaaga gcggccttat cttgcaaagt
     4861 ttgcaggata agctgggcga attatcgcgt cgaatgatcc attcctcccg tttttttgtc
     4921 cgggaagaac tttctgtagt gcagcagtta tcgattattc tcaatcacca ggccaaacgt
     4981 ttgctacaag cagaaaatca ctctgtggcc gaaaaaagtc aatacatcca catggcgtca
     5041 cccgaaaata tcctgaaacg ggggtataca ttgacattga aaaatggaaa aatcattaaa
     5101 tcggcaacgg agctttcgga ggatgatatt atcgagaccc tttttacaga tggaaaagca
     5161 gaatcaataa tcactaaaaa gtaatactgt catgacaaaa aaagatttct tcagaatcgt
     5221 gctaagaatt ctcggactct atttcttggc catggtggtt tttaactacc ttcccagcat
     5281 agtggtcttt tttcacagtc tgccccttct tagcctaatt ctggccatag ttgtggtttc
     5341 gctgttcgca tacatttttt tactgcttat tttgaagcct gatccagtta ttagaatttt
     5401 caagttagac aaaggattcg acgatgatgc gctgtctgca aagcgcatag aattcgtcaa
     5461 cttattaaaa atagctatta tagccacggg gttatttcta atcatcagcc acttgccaac
     5521 ctttttaact cacctccttt ttcttttaaa gctagtagta aagaaccgga acgaaataac
     5581 ggaccatgtg gaaagcgcgc tcctgaccga ttatatctct tggggaataa aaattttaag
     5641 tttgatagtc ggttatctga tgataacgaa ttactcggct attgctcggt ttatcataaa
     5701 aaaagattca gaaaaacagt cagataaatg aaagaatcag cccttaccta ctcacaggcc
     5761 aaacaggaac tcgaagagat tgtttcggcg attgaatcgg gggaattgga tgtggacgcg
     5821 ttgaccgaaa aagtgaaacg cgcatcggaa ctcatcgcat tctgtaaaga aagactcaca
     5881 aaaaccgatg aggagttaca gaaaatattg gatgaaatca attaagagtt tatgatcccg
     5941 gaacaacact tcagcgtagt tatcgttgcc ggcggcaaag gattgagggc aggcggagaa
     6001 atcccgaagc agttccagcc cattggcggg aaacccatat tgatgcgtac ggcagaagcc
     6061 ttttacaatt tcgattaccg gatgcgaatt gttattgttc tgccagagga gtttcgcttc
     6121 ttctgggaag aactttgtga aaagcacagt ttccggatac cacacagcat tgtcagtggg
     6181 ggagaaacac gcttccactc cgtgaaaaac ggactggaag aaatccagaa agacgagatt
     6241 gtagggattc acgatgccgc ccgaccgttc atccctaaag aagtgatcgg gagatgttac
     6301 cgggaagctt ttgattttcg ttgcggagtc attccggtta tcgaggaaaa gaatagtgtc
     6361 cgtattctta ctgcatacga gagtaaagct cttgaccgta aaaaaatccg tgtggtgcaa
     6421 actccgcagg tctttccggc cgatttgcta aaaaatgcct acgaaacacc ttaccgcgaa
     6481 gaatttaccg atgacgcttc agtggctgaa gacagtggaa ttcagataaa attggtgaaa
     6541 ggcgatgaaa gaaatataaa gattaccacc ccactcgatc tgaaatttgc agattattta
     6601 tgtcggaaca accttgtttg agtaactttt tgtaaacaat taagcggtta ttaaccccta
     6661 tttcgtacta aaagcactat tttattgaaa taaacttctc aaaatgctat tttattgtaa
     6721 ctttttctct atctttgccc taatatttta aaacgagttc ccatggaaaa aatcgacaaa
     6781 ctagacaggc aaatacttga aattatttca caaaatgccc gcatcccatt cagagatgtt
     6841 gcggatcaat gcggagtttc acgcgctgcc attcatcaac gtgttcagcg tatggtggaa
     6901 aacaatgtca taacaggatc aggttatcat gtcaatccca aaatactcgg atatgctgca
     6961 agcgcttata tcggtgtgaa actcgaaaaa ggatcgatgt acaagaacgt gattcccgag
     7021 tttgaaaaga ttcccgaagt aaccgagtgt cattttacta ctgggccata cactcttctt
     7081 ataaagcttt atgctaaaga caacgagcac ctaatggact tgctaaacaa caaaattcag
     7141 gagattcccg gtgttgtagc caccgaaacc atgatttcgc tgagccagag cgtaaaaaga
     7201 gaaattccta tcttaaaaga atctccacgc gaataaccaa aaacattaaa taaagttaaa
     7261 cagacggctt ttttcgtgca taaaaaaggt ttatgttata aatcgattta tatttgcgtc
     7321 gtattttgat tataaacttg aaataactat tcattaaaat attaaacaaa atggaagaca
     7381 aacaattaaa tgaaaaagaa agtctggaac ttatcactca gatgatccgc aacacgcaaa
     7441 agaaaatgga aaaagggagc ggtattccgt tcctgatttg gggctatgtc accatagccg
     7501 tttcattaac agtgtggtat ttactcggta aaaccggaaa tcagaactgg aatttcctct
     7561 ggtttgccat tccagttatc ggatttcccg taatgctgct cgccctgaag aggaaaacaa
     7621 ctcttcccaa aacgtatatc gacaaagtaa tcagctatgt atggattgtc atgggggttt
     7681 cggcgttgat cccttcgatg gctgcagccg taatacacaa tttcccggta ctgtttttgg
     7741 tagtcttgct tattagtgcc gggacggcta tgacgggact tgttatcaag tttaagccat
     7801 tggttatttc cggttttgga gggatgcttt tgtcgattct atgcttgatc cttcgcgaat
     7861 cgctagattc aatccttgtc tttgctgctc ttttcttgtt agtccaagtc attcccggac
     7921 atattttgaa ttataaaaac cgaaaaaacc atgtttaagg aactagatcc gctccttcat
     7981 tccgaacttc gactggcagt tatgtcaata ctgttgtcgg tagatgaagc cgattttgtt
     8041 tacctgaaag aacaaacaaa agcgacctcc ggcaacctga gtgtgcagat cgacaagctg
     8101 aacgaagctg gatacattga agtggagagg ggatttgcgg gaaaacgcac gcgtaccgtt
     8161 tgtaaaataa cgccggttgg cgtgaaagct tttgaagatt atgtggaatc tctaaaaagc
     8221 tatttgaagc tataagggaa agacaacgga aagagaatat aacttctaaa aaagacgaaa
     8281 gcctgaaact ttcgtctttt tatctttgtc gggatgaggt gaatcttttc ccacattttt
     8341 attttccgaa atatctgata atcaatacta catagtttca ctttaatacc tttttgtccc
     8401 gattgctgtc ccggtaaaaa ggcaaaaaaa ttagtcctgc ggaaacattt ggactatttt
     8461 tttaatttta cattggcagc ctatgcactg ccggcatttt ctcctccccc ttttgataaa
     8521 ttctcaatgg ttcgttgctg cgattcaatt atctttaata atctatcttc ttttatagct
     8581 attttaaaac cattgacggg atcttctgcc attgagctat cttcgctttg atttctatac
     8641 atttctccac gacctataag taaccagtca agactgtatt ggggaaaatt ttcagatatc
     8701 ttaatcagcc attttgtttg aatatctgtc ttgtttttca taggtttatg taaaacattc
     8761 ttgcttgcgc caattattga ctcaagtttg ctgacagtaa tattctctaa ttcaactatt
     8821 ttattaattc tatctaaata atcttccatt tatctgaaaa ttttcatcta attattttgt
     8881 ttatctgaaa attatcccct atatttgcga acgtaacatt tattacgtgc gaaattacgt
     8941 aaaatagatg atttaatatt caattcaaaa taaaattaat taaaatgaca tttacagacc
     9001 atgccaacag cctttccaac gagcgcgttg aattcataaa acgcattgca gagatcacgt
     9061 tggcctcacc ggccacggtt tcacgatgga tcagtggtga gtttattcct tccaaggtcc
     9121 gacgtactat tatatcaaaa gaactcaata tcccagaaga acaattattt ccacaaaaag
     9181 aaaatgaaac agtttagtct gacagaaac
//
LOCUS       DAOSDR010000073         9133 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrimonas sp. isolate AMR_MDS_1826
            NODE_2824_length_9133_cov_3.609055, whole genome shotgun sequence.
ACCESSION   DAOSDR010000073 DAOSDR010000000
VERSION     DAOSDR010000073.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863355
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrimonas sp. (wastewater metagenome)
  ORGANISM  Petrimonas sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Dysgonomonadaceae; Petrimonas.
REFERENCE   1  (bases 1 to 9133)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9133)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:10:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,720
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 35
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9133
                     /organism="Petrimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2824_length_9133_cov_3.609055"
                     /isolate="AMR_MDS_1826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2023866"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..408
                     /locus_tag="PLI30_04350"
     CDS             <1..408
                     /locus_tag="PLI30_04350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019539144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HOI78819.1"
                     /translation="TFTFSDGLSSKVKPSPGKEIVLVKRISHTAKSKVFEKNVLRNYI
                     YVKTMNELAKLCGYGCTKTFTRHFKKYFGQTPYQWMLDRKMEEIDFLVKNTELTITEI
                     AEMYGFKSLSHLVNFFSKRNGIPPHKSRSTEDT"
     gene            913..1491
                     /locus_tag="PLI30_04355"
     CDS             913..1491
                     /locus_tag="PLI30_04355"
                     /inference="COORDINATES: protein motif:HMM:NF027435.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4827 family protein"
                     /protein_id="HOI78820.1"
                     /translation="MKKTHYIIFCLLGLALVSTSCNKRKTYAEILKDETRAIEKFILE
                     NKLVVLDEFPTSLVFKTNEFYRDPATGVYFNIIEPGDTASKVKEGEEVYVRFIGLRYF
                     TSSNDTTEYSNLDPIRSPFPESLTYRGPVTVMNRSLYSGTTPGWAVPLTYVGHAGRVK
                     MIIPFNMGSQSDQQSYSTTYYDNVQYRFESQL"
     gene            1498..2880
                     /gene="glmM"
                     /locus_tag="PLI30_04360"
     CDS             1498..2880
                     /gene="glmM"
                     /locus_tag="PLI30_04360"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010802576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HOI78821.1"
                     /translation="MTLIKSISGIRGTIGGRVGDNLTPVDIVKFASAYALFIKKITGD
                     AKPKIVVGRDARMSGNMVHSLITGTLMGMGCDVVDVGMATTPTTEIAVVKESASGGII
                     ITASHNPKQWNALKLLNSRGEFLNADQGKEILEMAEAESFEYASVDELGKVSHKQYLH
                     AHIQSILQLDLVDLDAIKSARFRVAVDAVNSVGGNAVPELLYALGVKEVFKLHCAIHG
                     NFSHNPEPLPQNLTELSSLMKSSRADVGFAVDPDVDRLVIYAENGEPFGEEYTLVAVS
                     DYILQHTPGNTVSNLSSTRALRDITQRRGGDYFAAAVGEVNVVAKMKEVGAVIGGEGN
                     GGVIYPASHYGRDALVGIALFLTQLAKSGKKVSELRAEYPGYFMSKQKIELTPDIDTD
                     AILQKVKERFNGEQVTDIDGVKIDFPDKWVHLRKSNTEPIIRIYSEEKTQEEAEEVGK
                     QIIDIIKELK"
     gene            2973..3806
                     /locus_tag="PLI30_04365"
     CDS             2973..3806
                     /locus_tag="PLI30_04365"
                     /inference="COORDINATES: protein motif:HMM:NF039989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HOI78822.1"
                     /translation="MKERLISDDDIRKFHPVFRGKNGDRNIKWGMKLSGLNNACEVYD
                     KSKHLTGVAFCTDLLDKLGLKRTVVNDQVLEPFKDKPFIVVANHPNGHVDGIALIETV
                     ASRVKNFKVMVNFILGLVDTMEENFIKVNPYKHTDKMKHISLSGIKECIDHIRKGNSM
                     GFFPAGSVSRLKFRNGRFMIHDRHWQPSVIKLIQKARVPVIPLYIDCRNRYLFYATRF
                     LNWQLQSLVLCHELYNKKGKEMTLTFGEPVMPDVIRSISDADELGDFLRDKTYALAKK
                     R"
     gene            3823..5088
                     /locus_tag="PLI30_04370"
     CDS             3823..5088
                     /locus_tag="PLI30_04370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006842354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HOI78823.1"
                     /translation="MAKQNIQQVKQRFGIIGVSSELDRAIDIALQVAPTDLSVLITGE
                     SGVGKENFPQIIHQYSRRKHGPYFAINCGSIPEGTIDSELFGHEKGSFTGATATRKGY
                     FEVANGGTLFLDEVGELPLSTQARLLRVLETGEFIKVGSSQVEKSDVRVVAATNLNIA
                     QAIERGKFREDLFYRLNSVPIRISPLRDRKEDIALLFRKFASDCAEKYMMPSITLTED
                     AREMLTNFRWPGNVRQLKNVTEQISVIEHERVITSEILQKYLPKADAALPVLASQSRD
                     SDQKIFSSEREILYQVLFDMKKDINDLKNVVKTILEDAATSVPQDENPIFHSPAAPQD
                     KTPVTLPLKYEELMPKKNHLAEVHDRSDFQEATEVEITNLSLEDAEKEMIALALEKHD
                     GKRKLAASELGISERTLYRKIKEYNLEKL"
     gene            5085..5588
                     /locus_tag="PLI30_04375"
     CDS             5085..5588
                     /locus_tag="PLI30_04375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019540204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LptE family protein"
                     /protein_id="HOI78824.1"
                     /translation="MRKIRNTAVLFILLTSCSISYKFSGASIDYNLTKTLQLGTFVNQ
                     APLVYPPLESRFNEALKDMFTRNTRLQFVNQNGDMEIEGEIVGYELTPLAVQEDAFAA
                     ETRLTMNVRMRFRNNKIPGQDKEETISANRTFSSNVNLTDVQDQLIKELTDEIVDQIF
                     NTTMANW"
     gene            5590..6327
                     /locus_tag="PLI30_04380"
     CDS             5590..6327
                     /locus_tag="PLI30_04380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI78825.1"
                     /translation="MDKSDFFSYITDNRKLDERSLSGLENLVEEYPYFQTAHLLLAKN
                     RYLTRREEFEKNLAKTAVFCADRSRFFYFINNDKYARFFPKETVSSTVDRTQALLNAY
                     LDTFSEEPREIPEIETSIVSTDYFTYLQSLEEEPDGEIAEARQNEFKHQDIIDSFLEK
                     AAANEITITSLEREPAETQEVDAEPGNDGFLTETLAKIYIKQKKYEQALTIIRQLSLN
                     FPKKSVYFADQIRFLELLILNEKNKKE"
     gene            6330..6683
                     /gene="secG"
                     /locus_tag="PLI30_04385"
     CDS             6330..6683
                     /gene="secG"
                     /locus_tag="PLI30_04385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005865837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HOI78826.1"
                     /translation="MYIFITILILIAAILMILIVLVQNSKGGGLAAGFSSSNQIMGVR
                     KTTDFLEKATWTLAATILVLSILTAKFTTSRSTVPQSQVEVQQPAPLNPTTAPDVTPV
                     MPIQPDQQQATPPTE"
     gene            6842..7252
                     /gene="glmS"
                     /locus_tag="PLI30_04390"
     CDS             6842..7252
                     /gene="glmS"
                     /locus_tag="PLI30_04390"
                     /EC_number="5.4.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018358123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050097 - methylaspartate mutase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylaspartate mutase subunit S"
                     /protein_id="HOI78827.1"
                     /translation="MNEKTIVTGVIGADVHAVGNKILAFALEQAGFKVVNLGVMVAQE
                     EYIEAALETNADAILVSSLYGHGEIDCQGLREKCDEAGLKEIPLLAGGNLVVGKQNFE
                     EVEKRFLAMGFTRVYPPGTAVETSIADLKEILNI"
     gene            7317..8720
                     /gene="glmL"
                     /locus_tag="PLI30_04395"
     CDS             7317..8720
                     /gene="glmL"
                     /locus_tag="PLI30_04395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006744489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylaspartate mutase accessory protein GlmL"
                     /protein_id="HOI78828.1"
                     /translation="MNILTADIGSTYTKLTAIDTAKKEIVATSSAFTTIDTNVEEGFT
                     AAYNKLKRQAGAFRYDELLCCSSAAGGLKMVSLGLVPELTAKAAKLAAASAGGKVVKT
                     YAFEISQAEQAEIFDIRPDLVLLCGGTDGGNKEVILANARRLCQVGCPFTVVVAGNKC
                     ASFELEEIFRASGKPFLITENVMPEFNRLNIEPARRKIKELFISRIIEAKGLSRIQEM
                     CKTDIIPTPLAVLNACELLSKGTKNMPGLGDLLAVDIGGATTDVYSISDGRPTLENVT
                     VKGLPEPISKRTVEGDLGMRYSLPSLVDELDLDAFSKELSIDRSEVIGWVSTCTQHPG
                     LLAEAESREQKIEELIARNAVKIAVERHAGTYQPVYTPFGQVYTLTGKDLAAVPFVIG
                     IGGVVINARRPHAILEGAKRQPDDHVFAKPEQPGYLIDKKYIFASMGLLGSAYPDLAL
                     ELMKKETINLTHYGNFQ"
     gene            8704..>9133
                     /locus_tag="PLI30_04400"
     CDS             8704..>9133
                     /locus_tag="PLI30_04400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004364139.1"
                     /note="methylaspartate mutase subunit E; catalyzes the
                     formation of threo-beta-methyl-L-aspartate from
                     L-glutamate; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylaspartate mutase subunit E"
                     /protein_id="HOI78829.1"
                     /translation="MEISNKKLSTDAFFAERKEVLGHWHTGKGVDFDEAVAYQRSIPR
                     EKRFGLKLAQAAEQNVTLIQPRAGVALYEEHIELLRFLESEGEADLLPTTVDSYTRLN
                     RYNEAETGIEKSKETGRSMLNGFPIVNYGVDICRKVTSSLQ"
BASE COUNT         2685 a         1879 c         2213 g         2356 t
ORIGIN      
        1 acgtttacat tctcagacgg attgtcaagc aaagtgaagc cgtctcccgg taaagaaatt
       61 gtgttggtga aacggatatc acacactgca aaatcgaaag tatttgaaaa gaacgtattg
      121 agaaactata tatatgtgaa gactatgaat gaactggcga aactttgcgg ttacggttgc
      181 accaaaactt ttacgcgcca tttcaagaaa tatttcggac aaacaccgta tcaatggatg
      241 ttggaccgga aaatggagga gatagacttc ttggttaaaa atacggagtt gaccattacg
      301 gaaatagccg aaatgtacgg ttttaaaagc ctctcgcatt tggtgaattt tttcagcaag
      361 cggaacggta ttccacccca taaaagcaga tcaacagagg atacctgatt tctgtttttt
      421 acaacaaaca cccacttcaa acattccagt ccggatttgg tggagaaaat aaacctgcac
      481 aaaggcatcc tcatggtaat gaatgtcgaa aagatctttc tggggacggc caagcaaaga
      541 ccataagaat gtctgcaaga aatcctcttc tcagagaatt catctttaat gtttagacag
      601 aggagatgaa agacaggacc taatctttac catggttaag caaagatacg ggaccctata
      661 cgtagaggcc gtatcgttgc tgttttttga tcccagtgcg atttatccgg taatcgtctg
      721 ggatcatttt tttaagtata tgttacttta tcatcgaatt attgattaac attttctccc
      781 aaaaagtttt ttaaggacat aagagattaa ataaaatcaa cggtttatag tttattttgt
      841 gtgaaataac atttttttta aaggggattg cttacatttg tagccaattt ctaatttttc
      901 gtaattcaaa aaatgaaaaa aacacattac ataatattct gtttactggg gcttgcacta
      961 gtcagtactt cttgcaacaa aagaaaaacg tatgcggaga tcctgaagga tgaaacccgt
     1021 gccatcgaaa agtttatcct ggaaaataaa ctggttgtac tggatgaatt tcccacaagt
     1081 ttggtcttca aaaccaacga gttttaccgt gatccggcta caggagttta tttcaatatc
     1141 atcgagccgg gagatactgc cagcaaagta aaagaaggtg aggaggttta tgtcaggttt
     1201 atcggattgc gatacttcac ctcttcgaac gacaccaccg aatactctaa tctcgatccg
     1261 atacgtagcc cgtttcctga atcgctcaca tacagggggc ctgtaacggt aatgaaccga
     1321 tccctgtatt ccggaacaac acctggatgg gctgtcccgc ttacttatgt ggggcacgcc
     1381 ggaagagtaa aaatgattat ccctttcaat atgggttcac aaagtgacca gcagtcctat
     1441 tcaacaacct attacgataa cgttcaatac cgattcgaat cgcaattata acgtattatg
     1501 acacttatta aatccatatc cggtatcaga ggcacaattg gaggacgggt gggtgataac
     1561 cttactccgg ttgatatcgt gaaatttgca tcggcctatg ccctgtttat caaaaaaatt
     1621 accggagatg cgaagccgaa aatagtggtt ggcagagatg cgcggatgtc gggtaatatg
     1681 gtacatagcc ttattaccgg aaccttgatg ggtatgggtt gtgatgtggt ggatgtgggt
     1741 atggcaacaa cgcccaccac agaaatagcc gttgtgaaag aatcggcatc aggaggaatt
     1801 ataattactg ctagtcacaa tcctaaacaa tggaatgctc ttaaattgct gaacagccgg
     1861 ggcgaatttc tgaatgccga ccaaggcaaa gaaatactgg agatggctga ggcagaaagc
     1921 ttcgaatatg cttctgtgga tgaattggga aaagttagtc acaaacaata cctgcacgct
     1981 catattcaga gtattcttca attggacctg gtggatttag atgccataaa gtctgcccgc
     2041 ttcagggtgg ctgtggatgc ggtcaactct gttggcggaa acgccgtccc ggagttgttg
     2101 tatgccttgg gagtgaagga ggtttttaaa ttgcattgtg ccattcacgg taacttttcg
     2161 cataacccgg aaccgttgcc gcaaaacctg acggagcttt cgtcgctgat gaaaagctcc
     2221 cgggctgatg tgggttttgc cgttgatccg gatgtagacc ggttggtcat ttacgccgaa
     2281 aacggagagc cgttcggcga agagtatacg ttagtagctg tttcagatta tatattgcaa
     2341 catacccccg gaaatacagt ttctaacctg agttctaccc gggctttgcg ggacattacc
     2401 caacgacggg gcggtgatta ttttgctgct gcagtaggcg aagtgaatgt ggtggcaaag
     2461 atgaaagaag taggggctgt aattggcggc gaaggtaacg gcggagttat ttatccggcc
     2521 tcgcattacg ggcgtgatgc attagtaggc atcgctttgt ttttgacgca actggcgaaa
     2581 tcaggaaaaa aggtgagcga gctcagggcg gagtaccccg gttattttat gtcgaaacaa
     2641 aaaattgaac ttactcccga tatcgacacc gatgccatcc tgcaaaaagt gaaagagcgt
     2701 ttcaacgggg aacaggtgac ggatatcgat ggtgttaaaa ttgattttcc cgataaatgg
     2761 gtgcatttac gtaaatcgaa cacagaacct attattcgga tttattcgga agaaaaaact
     2821 caggaagaag cggaagaggt tgggaaacag attattgata ttatcaaaga actcaaataa
     2881 aagaaaggtt aatttcaaca attaccgtat attaagacac cttctgtttt agttgcggta
     2941 attttttgtt gaaaaacaga cgattgtata gcatgaaaga acgcttgatc agtgatgatg
     3001 acataagaaa gtttcacccg gtcttccggg ggaaaaatgg agataggaac attaaatggg
     3061 gaatgaaatt atcggggctc aacaatgcct gcgaggtcta cgataaatcc aaacacctga
     3121 cgggcgtggc tttctgcacc gatttgctag acaagttggg acttaagcgt accgttgtta
     3181 acgatcaggt tttggaaccc ttcaaggata agccgttcat tgtggtggcc aatcatccca
     3241 acggccatgt cgacggaatt gcgcttattg aaaccgttgc atcgagagtg aaaaacttta
     3301 aggtaatggt taactttatt ttggggctgg tggatacgat ggaagaaaac ttcataaaag
     3361 tgaatccata caaacataca gacaaaatga agcacatttc gctttcgggg ataaaagagt
     3421 gtatcgacca tatccggaaa gggaactcta tggggttttt tcctgcagga tcggtctcta
     3481 ggctaaagtt ccgcaacggt agatttatga ttcacgaccg tcactggcag ccgtcggtca
     3541 taaagctcat acaaaaggcc agagttcctg tgattccgct atacatcgat tgtaggaaca
     3601 ggtatctgtt ctacgctacc cggtttctca actggcaatt gcagagcctg gttctgtgtc
     3661 acgaactgta taacaagaaa gggaaagaaa tgacattaac atttggtgaa ccggttatgc
     3721 ccgacgtgat cagatcgatc agcgatgccg atgagttagg tgatttttta cgcgataaaa
     3781 cctatgcatt agcaaaaaag aggtaaattt gtgagtaaga acatggccaa acaaaatata
     3841 caacaagtta aacaacgttt cggcattatt ggtgtttctt ccgaattgga tagggccatc
     3901 gatatagctt tacaggttgc acctaccgac ctgtcggtgc ttattacggg tgagagtggt
     3961 gttgggaagg aaaacttccc gcagatcatt catcagtaca gtcgccgtaa gcacggaccc
     4021 tattttgcca ttaattgcgg gtctatcccc gaaggaacga tcgactccga gcttttcgga
     4081 cacgagaaag gatcgtttac gggtgctacg gcaacccgca agggatattt tgaagtggcc
     4141 aacggaggga cccttttcct tgatgaagta ggggagttgc cgttgtccac gcaggcccgt
     4201 ttattacgcg tgctggaaac cggagagttt ataaaagtag gttcttctca ggtggaaaag
     4261 agtgatgtcc gggtagttgc agctaccaac ctgaatattg cccaggccat tgaaagggga
     4321 aagttcaggg aagatctgtt ttaccggtta aattcggttc ctatccgcat ctctccgctg
     4381 cgtgacagaa aagaagatat cgcgttgttg ttccggaaat ttgctagtga ttgcgccgag
     4441 aaatacatga tgccatccat cacattgacc gaggatgccc gggaaatgct tacaaatttt
     4501 cgttggcccg gtaacgtcag gcaactgaag aatgtaacgg agcaaatatc ggtgattgaa
     4561 cacgaacgcg tgattaccag cgagattttg cagaaatacc tgcccaaagc agacgctgca
     4621 ttgcctgtgt tggcttcgca atcaagagat tcggatcaga aaatcttttc gtcggaacgg
     4681 gagattttat accaggtcct tttcgatatg aaaaaagaca tcaacgattt gaaaaatgtg
     4741 gtgaaaacta ttctggaaga tgcggcaact tcggttcccc aggatgagaa tcccattttc
     4801 cattcaccag ctgcccctca ggacaagacc ccggtgacgc tgccgttgaa atatgaagag
     4861 ctgatgccta aaaaaaatca tctggcggag gttcatgacc gatccgattt tcaggaggcg
     4921 accgaagttg aaattaccaa tttgtcactc gaagatgctg aaaaggagat gatcgcgttg
     4981 gccttggaga agcatgacgg gaagcgtaaa ttggccgcca gtgaactggg catttcggag
     5041 cgtacgttgt acagaaagat taaggagtac aatttagaga agttatgaga aaaataagaa
     5101 atacagcggt gctgtttatc ctgttgactt cctgttccat ttcttataag ttcagtgggg
     5161 cctccatcga ttataacctg accaaaacct tgcagctggg tactttcgtg aatcaggcgc
     5221 cgttggttta tccgccattg gagtcgcgct tcaacgaagc attgaaagat atgtttaccc
     5281 ggaatacccg gttgcagttt gtgaatcaga acggcgatat ggagatagaa ggtgaaattg
     5341 tgggttacga actaacaccg cttgccgtgc aagaagatgc gtttgctgca gagacccgct
     5401 taacgatgaa tgttcgtatg cgttttcgta acaataaaat cccgggacaa gataaagagg
     5461 agacaatctc tgccaaccgc accttctcca gtaacgttaa cctgacagac gtacaggatc
     5521 aattgattaa agagcttacc gatgaaattg tagatcagat tttcaacacg actatggcaa
     5581 actggtgaga tggataaatc ggattttttt agttatatca cggataacag gaagttggat
     5641 gaaagaagcc tgagcgggtt ggagaacctg gttgaagagt atccttattt tcaaactgcc
     5701 catttgctgt tggctaagaa ccggtattta acccgccggg aagaatttga aaagaacctg
     5761 gcaaaaactg ctgttttctg tgccgacaga agccgattct tttattttat caacaacgat
     5821 aaatatgccc ggttttttcc gaaagaaacg gtatcaagca ctgtcgacag aactcaagcc
     5881 ttgttgaacg cttacctgga tactttttcc gaagaacccc gggaaattcc ggaaattgaa
     5941 acaagcatcg tttctacgga ttatttcact tatctgcaga gcctggagga agagccggac
     6001 ggagaaattg cagaagcccg tcaaaacgaa tttaagcatc aagacatcat tgatagtttt
     6061 ttggaaaaag ctgcagctaa cgagattaca atcacttcgt tggaaagaga accggccgaa
     6121 actcaagagg tagatgcaga gccgggtaac gatggatttt taacggaaac gcttgcgaag
     6181 atttacatta aacaaaaaaa atatgaacaa gcattgacaa ttattcggca attaagtttg
     6241 aattttccaa aaaaaagtgt ttactttgcc gaccaaattc gatttttgga acttttgata
     6301 ctcaacgaga aaaataaaaa agaataaaca tgtatatttt tattaccatc cttattttaa
     6361 ttgccgccat cctgatgatc ctcattgtgt tggtgcaaaa ctccaaaggc ggtggactgg
     6421 cagcaggctt ttcatcatcg aaccaaataa tgggtgtacg caaaactacc gactttctgg
     6481 aaaaagcaac ttggactttg gctgctacta tccttgtttt aagtatacta acggcaaagt
     6541 ttaccacttc aagatcaacg gttccccaat ctcaggttga agttcaacag ccggcgccgc
     6601 tcaatccgac cacagcgccc gacgttactc cggtgatgcc catacagcct gaccagcaac
     6661 aggctacccc acccacagaa taatcgattc gattcgaaca atacaggaaa gagagaagct
     6721 tcacggtttc tcttttttcg tatgcggacc ttctaaaagt aaaaaagtta ttttcattta
     6781 aactttgtgg atatgtttta aagcattaat tttgcattgt actattaatg aaaaatactg
     6841 tatgaatgaa aaaactattg taaccggagt gattggcgct gatgtacatg ccgtaggcaa
     6901 taagattctc gcttttgctc tggaacaggc tggattcaaa gtggttaacc tgggcgttat
     6961 ggttgcacag gaagaataca tcgaagccgc actggaaact aacgccgatg ctattctggt
     7021 atcttcgctt tacggacatg gcgaaattga ttgccagggc ttgcgcgaaa aatgcgacga
     7081 agccgggctc aaagagattc cgcttttggc aggtggaaac ctggtggtag gaaaacaaaa
     7141 cttcgaagag gtagaaaagc gtttcctggc tatgggattt acccgggttt atccaccggg
     7201 tacggccgtg gaaacgagta ttgccgattt aaaagaaatt ctcaatatct gattcgacgt
     7261 tgcgtaacta tttcctcgca gctacccatt actattgaat aacgcgcagc atcaccatga
     7321 acattctcac tgcagatatc ggcagcacct ataccaaact gacagcaatt gataccgcca
     7381 agaaggagat tgttgcaacc tcctcagcct ttaccaccat cgataccaat gtggaggagg
     7441 gttttacggc tgcttataat aaattgaagc gacaggccgg agcgttccgc tacgacgaac
     7501 tgctttgttg cagcagtgct gccggagggt tgaaaatggt ttcgctgggg ctggttcccg
     7561 aacttaccgc taaggcggca aaacttgccg cagccagtgc gggaggcaaa gtggtcaaaa
     7621 catatgcttt tgaaatatcg caggcggaac aggcagaaat ttttgatatc cgtcccgacc
     7681 tggtgttgct ctgcggcgga accgatggcg gaaataagga agttattctt gccaatgcgc
     7741 gccggttgtg tcaggtcggg tgtccgttca ccgtggttgt agccggtaac aaatgcgcat
     7801 cgtttgagct ggaagagatt ttcagggcat cgggtaaacc gtttttgata accgaaaacg
     7861 ttatgcctga gttcaaccgg ttaaacattg aacctgcgcg gagaaaaata aaagaattgt
     7921 ttatcagccg catcattgaa gccaaagggc ttagccgcat tcaggaaatg tgcaaaaccg
     7981 atataatccc cacgccgtta gcggtgttaa atgcttgtga actgctgagc aaagggacta
     8041 aaaacatgcc aggattaggg gatttgctag ctgtggatat aggaggggcg accacggacg
     8101 tttactccat ctccgatggc aggcccaccc tcgaaaacgt taccgtgaaa ggacttccgg
     8161 aacccattag caaacgtacc gttgagggcg atctggggat gcgctacagc cttccgtcgt
     8221 tggtggacga actggacttg gatgcttttt cgaaggagtt aagcatcgac aggtcggagg
     8281 tgattggctg ggtaagcacc tgcacgcagc atcccggttt gttggcggaa gctgagtccc
     8341 gggaacaaaa gatcgaggag ctgatagcgc gtaatgccgt gaaaatagcc gtggaaaggc
     8401 acgctggtac ctatcagcca gtgtacactc ctttcggaca ggtgtatacc cttacaggaa
     8461 aagaccttgc cgcagtaccg tttgttatcg gtatcggagg ggttgttatc aatgcccgtc
     8521 ggcctcacgc tattctggaa ggcgctaagc ggcaaccgga cgatcacgtg tttgccaaac
     8581 ccgaacagcc cggttattta atcgataaaa aatatatttt tgcgtctatg ggattgcttg
     8641 gctcagctta tcccgatctg gcgttagaat tgatgaaaaa agaaacaata aatctgactc
     8701 attatggaaa tttccaataa aaaactttca actgatgctt tttttgccga acgtaaagaa
     8761 gttctcggac actggcacac cggaaaaggt gtcgatttcg acgaagccgt ggcctatcaa
     8821 cggtctatcc cccgggagaa acgttttggc ttaaagctgg cccaagctgc cgaacaaaac
     8881 gtaaccctta tccagccgcg tgcaggagtg gcgctttatg aggaacatat tgaactgctc
     8941 cggtttcttg agtctgaagg cgaagccgac ctgctgccca ccaccgtgga tagctacacg
     9001 cgcttaaacc gttacaacga agctgaaacg ggaattgaaa aaagcaaaga aaccgggcgt
     9061 tccatgttga acggcttccc tatcgtgaac tacggagttg atatatgtcg caaggtaacc
     9121 tcatcactcc aaa
//
LOCUS       DAOSDR010000074         9059 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrimonas sp. isolate AMR_MDS_1826
            NODE_2856_length_9059_cov_3.657263, whole genome shotgun sequence.
ACCESSION   DAOSDR010000074 DAOSDR010000000
VERSION     DAOSDR010000074.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863355
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrimonas sp. (wastewater metagenome)
  ORGANISM  Petrimonas sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Dysgonomonadaceae; Petrimonas.
REFERENCE   1  (bases 1 to 9059)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9059)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:10:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,720
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 35
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9059
                     /organism="Petrimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2856_length_9059_cov_3.657263"
                     /isolate="AMR_MDS_1826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2023866"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(392..1675)
                     /gene="eno"
                     /locus_tag="PLI30_04405"
     CDS             complement(392..1675)
                     /gene="eno"
                     /locus_tag="PLI30_04405"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007478314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HOI78830.1"
                     /translation="MRIVSILGREVLDSRGNPTVEVDVLTESGAFGRAAVPSGASTGE
                     NEALELRDGDKKRYLGKGVLKAVANINDVIAPALVGTNVLEQTQIDARLLELDGTKTK
                     SNLGANALLGVSLAVAKAAANYLGLPLYRYIGGTNTYVLPVPMMNIINGGSHSDAPIA
                     FQEFMIRPVGANSFREGLRMGAEVFHALKKVLHDKGLSTAVGDEGGFAPNLAGGTEEA
                     LESILTAIKNAGYEPGKDVKIALDCAASEFYKDGVYDYSKFEGPNGKKRTSAEQAEYL
                     QELITNYPIDSIEDGMHENDWDGWKLLTDKIGGQCQLVGDDLFVTNVDFLRKGIELGC
                     ANSILIKVNQIGTLTETLNAIEMAHRHGYTSVTSHRSGETEDATIADISVATNAGQIK
                     TGSLSRSDRMAKYNQLLRIEEELGDRAVYGYKKIR"
     gene            complement(1857..2468)
                     /locus_tag="PLI30_04410"
     CDS             complement(1857..2468)
                     /locus_tag="PLI30_04410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006800272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarC family protein"
                     /protein_id="HOI78831.1"
                     /translation="MLGFNFVEIISAFLVLFAIIDATGSVPIFLNLRSQNKTIEPFKA
                     SFYSTIILIMFLFIGEAILKLFQVDLSSFAIAGALVILIISIEMIFGVEIFKIDENTS
                     GNGSATLVPVVFPLITGPGTFTTLLAMRAEYNTINIIIAVILNIAFVYVVLRYLDGVK
                     RLMGVGGVFILRKFFGVILLAIAVRLITSNLNALITSIQSQAL"
     gene            complement(2536..3723)
                     /locus_tag="PLI30_04415"
     CDS             complement(2536..3723)
                     /locus_tag="PLI30_04415"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOI78832.1"
                     /translation="MKHWRAYLEIQKPEYLQTGLPQDVAADAVVVIPCYDEKDVLATL
                     RNLCQSATPKAKTVVTVIINSSVRSEKEVVLQNRKTYDEITAFAAENNRDILRFFPLI
                     FEDLPRKHAGVGLARKIGMDLAVEHFLNTQNPEGIIISLDADCTVSPNFLTGISTAYR
                     QTGPPRCTIQNFRHRIETNDKKLENAVRQYEQHIRYFSQMLRFIGFPYPHHTIGSAFS
                     VAADAYVRVGGMGRQQGGEDFYFLQKVFAFGRVERLDDVFVYPMARYSDRIPFGTGPA
                     LQKIADEPDGQMKTYHFNAFLALKDFFDLRQSFFKQDTGFIRQRISLLHPAIIEFSEK
                     NDVVSEIEECNRNSATFQAFEKRFFHHFNAFKIIKYLNFSHPTYFSYDALSRQIEKSN
                     RSF"
     gene            complement(3793..4461)
                     /locus_tag="PLI30_04420"
     CDS             complement(3793..4461)
                     /locus_tag="PLI30_04420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019538891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HOI78833.1"
                     /translation="MKNNNAYILGAILAVAILILYVLHFTSAPGKSGARKGDLITKMN
                     DSSVTLPVAYVNVDSLLMNYNFAKDLNEALMRTEESSRASLTQKERQLNAAAQEFQRK
                     LQNNAFLSQERAEQEQQRILRMQQEYQQMAERLGQEFALEQQKLNMELSDTVKVRLVE
                     FNKDKGYQIIYSNTGSDNILFADDKYDITKEVTEFLNKKYGPATSTSNTGAGTKTPAA
                     TETK"
     gene            complement(4483..4701)
                     /locus_tag="PLI30_04425"
     CDS             complement(4483..4701)
                     /locus_tag="PLI30_04425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019538892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI78834.1"
                     /translation="MIKTLLLGIAILFIAIMLMGIKVFFTKKGEFPNTHIGGSKAMRD
                     RGISCATSQDREASNRESLIEKIIKEKV"
     gene            4886..6355
                     /locus_tag="PLI30_04430"
     CDS             4886..6355
                     /locus_tag="PLI30_04430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008154611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-histidine dipeptidase"
                     /protein_id="HOI78835.1"
                     /translation="MNINELEPKIVWSYFHDITQIPRPSKKEEKIIAYLLNFAKEHNL
                     EAKKDKAGNVLITKEATPGKENVPTVILQSHVDMVCEKNSDVTHDFDNDPIETIIDGD
                     WVKANGTTLGADNGIGIAAQLAVLASNDIAHGKIEALFTVDEETGLTGANSLEPGFIT
                     GNVLLNLDTEEEGEIYIGCAGGKGTRAYFKYKEKDAPKKYFWFKIQVKGLRGGHSGSD
                     IDKGLGNANKILNRFLYSIMRKKYGMVLSEIGGGNLHNAIAREAYAVVGVKDKYKEDM
                     RVKLNVFLANVQNEYKKTDPNLDIQLESVQMPSKIIKKGVAEKLIHSLYACPHGVIGM
                     SFDIDGLVETSTNLASVKMLENYTIEVGTSQRSSVESRKDDIVNTVSAVFELAGAKVV
                     HSEGYPGWQPNTDSQILKLAKKEYKNLYNKNAKVKAIHAGLECGLFLEKYPQLDMISI
                     GPDMTDVHSPDEKMNIPSVGKFWDYLVRILESVPAEGEE"
     gene            6422..7462
                     /locus_tag="PLI30_04435"
     CDS             6422..7462
                     /locus_tag="PLI30_04435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008154609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI78836.1"
                     /translation="MRKILLMVFFFSCVLFSFSQTGFRIMFYNVENLFDTIDDPLKND
                     DEFLPDGPMNWQPWKYWEKLRNITRVITAIGGMYSPALVGLCEIENDSVVYDLARRSP
                     LRAQGYEYIVTHSPDERGMDVALLYQRHQFNLLDKNEYEVVFSDKVVRPTRNILHAAG
                     RIVSGDTLDVFVCHWPSRSGGQRESESARLDAATLLRRKVDSLFMIRKSTHIVIMGDF
                     NDGPHDKSIFRMLKAKSRRHYRSDKELYNLFFHRLKEKDFGTYFFRGRWEVLDQFIVS
                     GNLLREDNRIYIKGGEAHIFKPDFLLQEDKASGIKRPYRTHYGPRYVGGFSDHLPVYL
                     DIVINSCPDSVR"
     gene            7649..>9059
                     /locus_tag="PLI30_04440"
     CDS             7649..>9059
                     /locus_tag="PLI30_04440"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005822256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HOI78837.1"
                     /translation="MTNPIIKRIELSDGRTITLETGKLAKQADGAVELRMGNTMLLAT
                     VCAAKDAAPGVDFMPLQVEYKEKFSAFGRFPGGFMKREGRPSDYEILTSRLIDRALRP
                     LFPDDYHAEVFVTVMLFSADGEDMPDALAGLAASAALAVSDIPFNGPLSEVRVARVDG
                     QLFVNPTFSQLEKADMDIMVGATIDNIMMVEGEMSEVSEAELLEAIKFAHEEIKKQCQ
                     AQLELMELCGTVKKREYSHEENDEELRKQVWSICYPKAYVVASSQNSDKHARMDAFET
                     ILEEFMESVPEEERETKAPLVAKYYHDVEKEAMRRCILDEGKRLDGRSTTDIRPIWSE
                     VDYVQGPHGSAVFTRGETQSLTTVTLGTKLDEKIVDDVLNHSTERFLLHYNFPPFSTG
                     EARPQRGTGRREIGHGNLAHRALKRMIPEGYPYVIRVVSDILESNGSSSMATVCAGTL
                     ALMDAGVQMKKPVSGIAMGL"
BASE COUNT         2448 a         1960 c         2144 g         2507 t
ORIGIN      
        1 acccgcgaac atcatctctc cgggaacatc aaccgatgcc gtcatggaga gtacttgcgg
       61 acgttccagt tctactttct ccgaatcgtt gttcgacaag gtcataactg tgataatact
      121 cacggctaca atagctaaaa aaatgtatat gatgtttctt ttcatcggta aggaatgtta
      181 taatacggat ttatttgtgt caaatataca actatattaa aaatactcca actattcatt
      241 aaactttctc atgcgttgcg atcgattcat gggttctctt ataattagcg ttgattggtt
      301 agggtgtaac ccaagcagga tacaggacag aaagaaaaaa gggcatgacg gcaaagccgg
      361 cacacccttt cactgttgat ggttttttct cttatctgat tttcttgtat ccgtaaacgg
      421 ctctgtcgcc caattcttct tcaatgcgga gcagctggtt gtatttagcc atacggtctg
      481 agcggctcag cgatcctgtt ttgatttgtc cggcattggt agctacggat atatcggcga
      541 tggtagcatc ttcggtttcg cctgagcggt gagatgttac gctggtgtat ccgtggcggt
      601 gtgccatctc gatggcgttc aacgtttcgg tcagtgtccc gatctggttt accttgataa
      661 gaatggagtt tgcacaaccc agttcaatcc ctttgcgcag gaagtcgacg tttgttacaa
      721 acaaatcgtc acctaccagt tggcattgcc ctccgatttt atcggtcaga agtttccatc
      781 cgtcccagtc gttttcgtgc atcccgtctt cgatggagtc gatcgggtag ttggtgataa
      841 gttcctgcag gtattcagct tgctcggccg aagtacgttt tttaccgttg gggccttcaa
      901 atttggagta atcgtaaacg ccgtctttat agaattcaga agcagcacag tcgagagcaa
      961 tctttacatc tttacccggc tcgtagcctg catttttaat agcggtaaga atggattcca
     1021 atgcttcttc ggttcctccg gccaggttcg gggcaaagcc gccttcatct cctacagcag
     1081 tgctgagccc tttgtcgtga agcacttttt tgagtgcatg gaaaacctcg gcacccatcc
     1141 gaagtccttc gcggaaagaa ttcgctccaa ccggacgaat cataaattcc tggaaagcga
     1201 tgggggcgtc ggagtgagaa ccgccgttga tgatattcat catgggaacg ggcaacacgt
     1261 aagtatttgt tccgccgatg tagcgataca ggggcaatcc taaataattg gctgcagcct
     1321 ttgctacggc aagcgaaacg cccagaagtg cgttggcacc taagttcgat ttggtttttg
     1381 ttccgtcgag ctcgagtagc ctggcatcga tttgtgtttg ttccagtaca tttgttccca
     1441 ccaatgccgg ggcaataaca tcgttgatgt ttgccaccgc tttcaaaaca cctttaccca
     1501 ggtatctctt tttgtcgcca tcgcgaagct ccagcgcttc gttttcaccg gttgatgctc
     1561 ccgaagggac tgcagcgcgt ccaaacgcac ccgactccgt taatacgtca acctctactg
     1621 tgggattgcc tctggaatcc aacacttcac gtcctaaaat acttacaatt ctcatgtcgt
     1681 ttttaatttt tatattgaaa aatttagttc taaatgattg tttaataacc taatattagc
     1741 gaaaagtctg ttttacaaat taacgtacaa atatacgttt tttttgttta tccggccggg
     1801 gttctgacgt taaatattgc ttacgcagca aataatttca tgctgcaatc ttatgtttat
     1861 aacgcctgcg actggattga tgttatcagc gcattaaggt tcgacgtgat gagacgaaca
     1921 gcaatagcta agaggattac cccgaaaaat ttacgtaaaa taaaaacgcc gcctaccccc
     1981 atcaatcttt tcactccatc taaataacgc aacaccacat agacaaaagc aatgttcagg
     2041 atgacagcga tgataatgtt gatcgtgttg tactcggcac gcatagccaa aagcgttgtg
     2101 aaggtcccgg ggccggtaat cagtggaaaa accaccggta caagcgttgc cgaaccgttt
     2161 ccggaagtgt tttcgtcaat tttaaatatt tctacgccga aaatcatttc gatggaaata
     2221 atcaaaatca ccagtgcgcc agcgattgcg aacgacgaaa gatccacctg gaaaagtttg
     2281 agtatggcct ctcctatgaa aagaaacatg atgaggatga tggtcgaata aaacgaggcc
     2341 ttgaacggct caatggtttt attctgactc ctaaggttca ggaatatagg aactgagccc
     2401 gttgcgtcaa taatggcgaa caggaccagg aaggcactga ttatttctac aaaattaaat
     2461 ccgagcatac cgggaacatt ttttttgata acctttgtta aaccgggtgc aaagttatta
     2521 aaaaagagcc aattctcaaa atgaacggtt cgatttctcg atctgtcggg aaagggcgtc
     2581 gtaagaaaag tatgttgggt gggaaaaatt caggtatttt atgatcttga aagcattgaa
     2641 atggtgaaaa aatcgttttt cgaaggcttg aaatgtggca ctgttccggt tgcattcttc
     2701 aatctcggaa accacatcgt tcttttcaga aaattcaatt atcgccggat gcaacaggga
     2761 tattctctgc cggataaacc cggtgtcctg tttaaagaaa gattgccgca ggtcaaaaaa
     2821 atcttttaac gccagaaaag cgttgaagtg ataagttttc atctgtccat caggttcgtc
     2881 tgcaattttt tgcaacgccg ggcctgtacc gaacgggatc ctgtcggaat accgtgccat
     2941 gggataaaca aaaacatcgt ccagcctttc gactcttccg aatgcaaaaa ctttttgcag
     3001 gaaataaaaa tcttcaccgc cctgttgtcg tcccattcct ccgacacgta catacgcatc
     3061 cgctgcaact gaaaatgccg agccgatagt gtgatgcggg tatggaaatc caataaaccg
     3121 cagcatttgg ctgaaataac ggatgtgttg ctcgtattgg cgaacggcgt tttcgagttt
     3181 tttatcgttg gtttcaatcc ggtgacggaa attctgtatt gtacagcggg gagggcctgt
     3241 ttgccggtag gcagtcgaaa taccggtcag gaaattagga gagacggtac agtcggcatc
     3301 cagggagatt attatccctt ccgggttttg tgtgttcagg aaatgttcga cagccaggtc
     3361 cattccgatt tttcgggcaa gacccacgcc ggcatgtttt cggggaagat cttcgaagat
     3421 caacgggaaa aaccgaagga tgtcccggtt gttttcagca gcaaatgcag tgatttcgtc
     3481 gtatgttttt ctgttttgaa gaacaacttc cttttccgaa cggacacttg agttaatgat
     3541 cactgtcacc actgttttcg cttttggtgt tgcagactgg caaagattcc gcaaggtggc
     3601 caacacgtct ttctcatcgt aacacggtat aaccacgacc gcgtcggcag caacatcctg
     3661 tggaagcccg gtttgcaggt actcgggttt ttgtatttcc agataggctc gccagtgttt
     3721 catgatgcaa aaataaaaaa taaggttctt tttgcaaaga accttactgt attcatttga
     3781 tcaggaagat tattacttgg tttcggttgc agcgggagtt tttgttcctg ctcccgtgtt
     3841 gctcgtagaa gtcgcagggc cgtacttttt gttcaggaat tcggtcactt ctttggtaat
     3901 atcgtatttg tcgtccgcaa aaagaatgtt atcggatccc gtgttgctgt agatgatctg
     3961 atagccttta tccttgttga attccactaa acgaaccttt actgtatcgg ataattccat
     4021 attgagtttt tgctgttcaa gagcaaattc ctgacccaac ctttcagcca tttgctgata
     4081 ttcctgctgc attcttaaga tgcgttgctg ctcctgctca gctctttcct ggctcaggaa
     4141 tgcgttgttt tgaagcttgc gctgaaattc ctgtgcagct gcattcagtt gacgttcttt
     4201 ttgtgtcagg cttgcacgcg agctctcttc ggttctcatc aaggcttcgt tcaggtcttt
     4261 tgcgaagtta taattcatca gaagagaatc tacattcaca taggcaacgg gtagggtaac
     4321 ggaagaatcg ttcatcttcg taatcaaatc tccttttctg gcgccgcttt ttcccggtgc
     4381 ggaagtaaaa tgcaagacgt ataaaataag gattgcaaca gctaagattg cccccaaaat
     4441 ataggcatta ttattcttca ttttctcaaa aaattgtgta ggttagactt tttcttttat
     4501 tattttttca atcagacttt ctctgttcga tgcttctctg tcttgcgatg tggcacagct
     4561 tatacctctg tctctcatag ccttacttcc gccaatatgt gtgttgggaa actcgccttt
     4621 ttttgtaaaa aaaactttta tacccattag cataatggct ataaaaagga tagcgatacc
     4681 caggagtaaa gtttttatca tttaattctt aattttgtgg cgcaaagata gtttaaatag
     4741 ccaattaaaa ctcatttttc gtattaaaga tgctaaaatt ttctgttttt tgcgattttc
     4801 attcctgttt tccgactaaa gcataaacaa gaaagcattt tatatgttaa tatagattat
     4861 acagttattt taaaaaacag atcgtatgaa tattaatgag cttgagccga aaatcgtatg
     4921 gagttatttt cacgacatca cacaaattcc acggccatcg aaaaaagaag agaaaattat
     4981 cgcttatctg cttaactttg ccaaggagca caaccttgag gccaagaaag ataaagccgg
     5041 taatgtgctg atcacgaaag aagcaacgcc gggcaaagaa aatgtgccga cagttattct
     5101 gcagtcgcat gtggatatgg tgtgcgagaa aaacagcgat gttacgcacg atttcgataa
     5161 cgacccgatt gaaactatca tcgatggcga ctgggttaaa gcaaacggga caactctcgg
     5221 ggcggataac ggtatcggga ttgccgcaca gttggcagtg cttgcctcaa acgatatcgc
     5281 tcatggcaag atcgaagcgt tgtttaccgt ggatgaggaa accggattga ccggagccaa
     5341 ttcgttggag cccggtttta ttaccgggaa cgttttattg aacctggata cggaggaaga
     5401 gggtgagatt tatatcggtt gtgccggagg aaaagggaca agagcctact ttaagtataa
     5461 agaaaaggat gcgcccaaaa agtacttctg gtttaaaatt caggtgaaag gacttcgtgg
     5521 cggacattca ggaagcgata ttgataaagg gttgggtaac gccaacaaaa tcctgaaccg
     5581 gtttttgtac tccattatgc gcaagaagta cggtatggtg ctatcagaaa ttggtggagg
     5641 aaacctccac aatgctattg cgcgtgaagc gtatgcagtg gtgggtgtaa aagataaata
     5701 caaagaagat atgcgggtga aactgaatgt cttccttgcc aatgtccaaa atgaatacaa
     5761 aaaaacggat cccaacctgg atatccagct cgaatcggta cagatgccct ctaagattat
     5821 aaaaaaaggt gttgctgaaa aactgataca ttccctgtat gcctgtccac acggggtaat
     5881 cggtatgagt tttgatatcg acggattggt ggagacctcc accaacctgg catcagtgaa
     5941 aatgctggaa aattatacta tcgaggtggg taccagccag cgtagttcgg tggagtcacg
     6001 caaagacgac atcgtcaata cggtttccgc cgtctttgaa ctggccggtg caaaagtggt
     6061 gcacagcgaa ggatatccgg gttggcaacc gaataccgat tcccagattc tgaaactggc
     6121 aaagaaagaa tacaagaacc tttacaataa aaacgccaag gtaaaagcta ttcatgccgg
     6181 cctggaatgt gggctgtttc tagagaaata ccctcagttg gatatgattt ccattggtcc
     6241 tgatatgacg gatgtccatt cgcccgatga aaaaatgaat attccctctg tgggaaaatt
     6301 ttgggattac ctggtccgga tcctggaaag tgtccctgcc gaaggggagg aataaatcaa
     6361 tagttatctt tgatgcttcg gtggcgttct tgatgacaaa atatggagat tgtgtcgtgt
     6421 tatgagaaaa attctcctga tggtcttttt tttttcttgt gtgctgtttt ccttttcaca
     6481 aacaggcttc cgcattatgt tttataacgt ggaaaacctt tttgacacca tcgatgatcc
     6541 gctgaaaaac gacgatgagt ttctccccga tggccccatg aactggcagc cctggaaata
     6601 ctgggagaag ctcagaaaca taacgcgtgt aattactgcc attggcggta tgtattcccc
     6661 cgccctggtg gggctttgcg aaattgagaa cgacagcgtt gtgtatgatc ttgcccggcg
     6721 gtcgccactt cgggcgcaag ggtatgaata tatcgtcaca cactctcccg atgagcgtgg
     6781 tatggatgtg gctttgcttt atcagcggca tcaattcaac ctgctggata aaaatgaata
     6841 cgaggttgtt ttcagcgata aagttgtacg ccccacacga aatatactgc acgctgccgg
     6901 gagaatcgtt tctggtgata ctcttgatgt ttttgtttgt cattggccca gccggagtgg
     6961 tggccagcgc gaatccgagt ctgcccggct ggatgcggca acccttttgc gaaggaaggt
     7021 cgacagcctc tttatgatca gaaaaagcac ccatatcgta attatgggag atttcaatga
     7081 cggcccgcat gacaaaagca tcttccggat gctgaaagca aagagccgac gtcattaccg
     7141 ttccgacaaa gaattgtaca acctgttttt tcaccggctg aaagagaaag acttcggcac
     7201 ctatttcttt cggggaaggt gggaggtgct ggatcagttc atcgtgagcg gaaatctgtt
     7261 gagggaggac aacaggatat atatcaaggg aggtgaagcg catatcttca agcctgactt
     7321 tctgctgcag gaagacaaag cctccggcat aaaacgtcct taccgaaccc attacggccc
     7381 gaggtatgtc ggcggtttca gcgaccattt gccggtttat cttgatattg taataaactc
     7441 ttgtccggat agtgtacgtt aagcaaaatg ggtagagtcg aactcggttg aggcatgccc
     7501 tagcggagaa atgaataagt aaaaccatat tattcgtaaa attcaaaaca gtccctcaat
     7561 attttatggt tttatacgct tcaaaacgaa aactttatct tatctttgtg ggattttaca
     7621 tccaaaattt aaaaataata atttatttat gacgaatcca atcatcaagc gaatcgagct
     7681 gtcagacgga cgcacgatta cgctcgaaac gggaaaattg gcgaaacaag ctgatggggc
     7741 cgtggagttg cgtatgggca acaccatgct gctggccact gtttgtgctg ccaaagacgc
     7801 tgcccccgga gtagatttta tgccgctgca agtagaatat aaagaaaaat tctccgcttt
     7861 cggaagattt ccgggcggat ttatgaaaag agaaggaagg ccctcagact acgaaattct
     7921 taccagccgg ttgatcgacc gtgccctccg cccacttttc cccgatgatt accacgctga
     7981 agtttttgta accgtcatgc ttttctcggc tgatggtgaa gatatgcctg acgcattagc
     8041 cgggttggct gcttctgccg cattggccgt ttcagatatt ccgttcaacg gtccgctatc
     8101 agaagtacgg gttgcccgtg tcgacggaca gctctttgta aaccctactt tcagtcagtt
     8161 ggaaaaagcc gacatggaca ttatggtggg tgccaccatc gacaacatta tgatggtgga
     8221 aggagaaatg agcgaggttt ctgaagccga attgctcgaa gccattaagt tcgctcacga
     8281 agaaattaaa aaacagtgtc aggctcagct tgaactgatg gaactttgcg gaacggttaa
     8341 gaaacgtgaa tattcacacg aagagaacga cgaagaactc cgcaaacagg tgtggagcat
     8401 ctgttatccc aaagcgtatg tcgttgcttc ttcgcagaac agcgacaagc atgcgcgcat
     8461 ggatgctttc gagactatcc tcgaagaatt catggaatcc gttcccgaag aagaacggga
     8521 aacaaaagcc ccgctcgttg ccaagtatta ccacgacgtg gaaaaagaag cgatgcgccg
     8581 ctgcatcctg gatgagggaa aacgtttgga cggaaggagc acgaccgata tacgccccat
     8641 ttggagtgag gtggattatg ttcaaggacc acacggatca gcagtgttca cccgcggtga
     8701 aacgcaatcg ctcacaacgg ttaccctggg tacgaaatta gatgagaaaa ttgtcgacga
     8761 cgtgctcaac cacagcacag agcggttctt gttacactat aatttccctc cattctcaac
     8821 gggagaagcc cgtccgcaac gcggtacagg acgtcgtgag atcggtcacg ggaacttggc
     8881 acatcgcgcc ctgaaacgca tgattccgga aggatacccg tacgttattc gtgtcgtttc
     8941 cgatattctg gagtccaacg gatcttcgtc tatggcaacg gtttgcgccg gtacgctggc
     9001 cctgatggat gccggagtgc aaatgaaaaa gccggtatcg ggtatcgcta tgggactga
//
LOCUS       DAOSDR010000075         9035 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrimonas sp. isolate AMR_MDS_1826
            NODE_2863_length_9035_cov_4.297327, whole genome shotgun sequence.
ACCESSION   DAOSDR010000075 DAOSDR010000000
VERSION     DAOSDR010000075.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863355
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrimonas sp. (wastewater metagenome)
  ORGANISM  Petrimonas sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Dysgonomonadaceae; Petrimonas.
REFERENCE   1  (bases 1 to 9035)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9035)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:10:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,720
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 35
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9035
                     /organism="Petrimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2863_length_9035_cov_4.297327"
                     /isolate="AMR_MDS_1826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2023866"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..406)
                     /locus_tag="PLI30_04445"
     CDS             complement(<1..406)
                     /locus_tag="PLI30_04445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018336988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOI78838.1"
                     /translation="MQFEYDIQGGDFSSAGKASSDIKKILKQLNIDPQVVRRIAVALY
                     EAEVNVVAHAYKGVMRVDLDAQKIKILLEDEGPGIENIERAMQEGFSTASEEVRQMGF
                     GAGMGLPNIKRNTDEMHLTSVPGKGTTLEMTVK"
     gene            complement(408..749)
                     /locus_tag="PLI30_04450"
     CDS             complement(408..749)
                     /locus_tag="PLI30_04450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019541389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HOI78839.1"
                     /translation="MNLYTVNELVKGTICQECSTSQEFTYAFASDLMSDVLRFNMEKT
                     ILITGLSTVQTLRTAEMSNIECIIFARDKKVTDEMITLAAENNISLITTPLTLFEVSG
                     ILYSNGIKPIF"
     gene            complement(994..2751)
                     /locus_tag="PLI30_04455"
     CDS             complement(994..2751)
                     /locus_tag="PLI30_04455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005648987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent [FeFe] hydrogenase, group A6"
                     /protein_id="HOI78840.1"
                     /translation="MEININNRPVQVAEGATILEACRSVGIEVPTLCYLKDVSQNASC
                     GVCVVEVKGAKSLLRSCITQVTEGMEISTNSPRAMQARKVNVELLLANHPQDCLICDR
                     NGNCELQELTHALGISARRFVRTRKELLVKDETSLSLVRDPEKCILCGRCVAVCSQMQ
                     GVKAIDFSGRGLKSKISTFLDSGLGLVACSNCGQCALVCPTGAITERSSVSEVWAALQ
                     DPGKIVLVQTAPAVRVGIGEAMGMPYGSLVTGQMVAGLRRLGFSKVFDTNFAADLTII
                     EEGNELLHRIRTGGELPMITSCSPGWIKFIEDFYPGLLRHLSTCKSPQQMFGAVAKTY
                     YAEKTGVDPRSIVVVSIMPCTAKKYEAKRPEMDGAFHYWKEKLKLKDEEHFFDVDFSL
                     TTRELARMFSESGVNFSSLPEERFDSPLGESTGAAVIFGATGGVMEAALRTAYEVYTG
                     KTLHDVNFTSVRGFQGIKEADVNLNGTVIKVAVAHTLKNAGILLEQIKNGTSPYVFIE
                     VMTCPGGCLGGGGQPIPTNAETREKRAESIYYDDDHKAIRKSHENPEITAIYADFLKK
                     PLGEKSHHLLHTHYMKREY"
     gene            complement(2769..5903)
                     /locus_tag="PLI30_04460"
     CDS             complement(2769..5903)
                     /locus_tag="PLI30_04460"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOI78841.1"
                     /translation="MKPSEIETLRKQHKERILPATPMILVGMGTCGIGNGAATVYHHF
                     EKVLSENNFDCKLKQAGCFGFCAEEPMVMFYQPGKPMLVYSKVEVKDVKRIIDNLHKN
                     RLYKNKLLCRIDEWDFLTSEVVFGKGYEDIPHWDEVPFFKGQKKVVLRHAGLIDPEDI
                     FDYIAVGGYNALAKAVMAYEPAEVVEQVMASKLRGRGGAGFPTGVKWSIMQKQDARQK
                     YVICNADEGDPGAYMNRNEIESDPHMLLEGMLIGAYAMGASEGIAYVRAEYPLAVKRL
                     RTAIGQAEQLGLMGENILGTGFSFKMDIVEGAGAFVCGEETALIASIEGKAGRPLPRP
                     PYPASKGLYGKPTNINNVETWCNVPVIIEKGAGWFTAMGTEKSPGTKVFSLVGKVKNT
                     GLVELPLGSRLEQFVFNIGEGTGTSKRVKAVQTGGPSGGCIPLEFFSTPVDYESLAGI
                     GAIMGSGGMVVMDQDNCMVDVARYFLEFNAGESCGKCTPCREGASQALAILTRITRGQ
                     GTFADLTVLELLSGVIKDSSLCALGQTAPNPILTTLKYFRNEYEEHIREKRCHAGTCE
                     SLFMALCENSCPLHMNIPGYIELIKEDRVEEAFELTLRDNPLPGTIGRICHFHCQMRC
                     RREMLDQPVSQGEIHRYLADTMYQLGKENGIYHKLIKEKLPPTGKRIAIVGAGPAGLT
                     AAFYLVRLGHDITVYEALSEAGGVARFGIPHYRLPKEVLRKEARLIKKLGVKFKFNQR
                     LGDNLSLEELKNEFDVIYLAIGAWKNIDLKIPGEDAKGVFAGTTILKEMAVGKTPKVG
                     ERVAIVGAGNVAIDTARSLLRLGKDVTIVYRREKGDMPANAVEISESEEEKINYRFMA
                     SPNGIITDKSGRVKALHIERMSGGSIDSSGRKKPVATGVFEEIPCDAVVLAVGERVDS
                     GFLAEKNMKLTRDGRIVIDPFTFQTTDPKVYAGGDAVSGPSTAAEAMGMAKKAAAAMD
                     RMLMGENRFCLLSPPFDYRNVVPANPKPASKNVPRRLSVKDRVNNFNEISCGFTGEQA
                     RNEVERCLRCDVKY"
     gene            complement(5900..6412)
                     /locus_tag="PLI30_04465"
     CDS             complement(5900..6412)
                     /locus_tag="PLI30_04465"
                     /inference="COORDINATES: protein motif:HMM:NF013426.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HOI78842.1"
                     /translation="METLINAPVTGEIFDEIIEKYRGKLGMLLTTLEEAQEKNPLNYL
                     PGETLAEISKKLKVPYTQVYSVATFYSFFNLKPQGKHSIVVCRGTACHTKGSKALLDD
                     IGKILGIKRTGDDTESSFTTPDNMFTIRTVACFGQCAQSPVVAIDNVIYSNVNSRKLM
                     KILSKYTPRL"
     gene            6802..8550
                     /gene="lysS"
                     /locus_tag="PLI30_04470"
     CDS             6802..8550
                     /gene="lysS"
                     /locus_tag="PLI30_04470"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019541388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HOI78843.1"
                     /translation="MHHLELSEQEIIRRQSLNELRNLGIEPYPAALYEVNAYSTDIKE
                     EFNDEEKRPVSIAGRIMNRRIMGKASFIELQDSKGRIQVYITRDDICPGEDKELYNTV
                     FKKLLDIGDFIGITGFVFRTQMGEISVHAESLTVLAKSLKPLPVVKMKDGVAYDKFND
                     PELRYRQRYVDLVVNDEVKDIFIKRTRIFNAMREFINKFGYLEVDTPVLQSIPGGAAA
                     RPFITHHNALDIDLYLRIANELYLKRLIVGGFEGVYEFSRDFRNEGMDRTHNPEFTVM
                     EIYVSYKDYKWMMEFTEQMLEQIATKVLGTTKTTIGDREIDFKAPYPRVTMIDAIKEH
                     TGMDITGMDEAQLREVCRKLNIEVDETMGKGKLIDEIFGEKCEGNYIQPTFIIDYPIE
                     MSPLCKRHRANPELTERFELMVNGKELANAYSELNDPIDQRERFEEQLRLSEKGDDEA
                     MFIDQDFLRALEYGMPPTSGMGIGMDRLTMLLTGQTTIQEVLLFPQMRPEKKTKKDST
                     SKYVETGIPEAWVAVLQKAGYNEVKSLTDVNPNKLHQEICGLNKKFKMELNNPSVEDV
                     KSWVENAKKNESLNAQ"
     gene            8547..>9035
                     /locus_tag="PLI30_04475"
     CDS             8547..>9035
                     /locus_tag="PLI30_04475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019541387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HOI78844.1"
                     /translation="MSSLGKIAILGGGTWATALAKIVLMNEKHINWFIRRDDQIEGFY
                     KLGRNPSYLTNVKFNLAQITFYSNIEKAIKDSDTIIIAIPSPYVKQYMRKIWMSSTLK
                     GKFVVSAVKGMIPNDNIVVSEYLMSNFKIPRENIGVISGPCHAEEVALERDPSCEVCG
                     GKN"
BASE COUNT         2367 a         2350 c         1919 g         2399 t
ORIGIN      
        1 acttcactgt catctccaac gtagtcccct tgcctggaac cgatgtgagg tgcatttcat
       61 cggtattccg ctttatattg ggcaatccca ttcctgcacc gaatcccatt tggcggactt
      121 cctccgatgc tgtggaaaaa ccttcctgca tggccctttc aatattttca atgccggggc
      181 cttcatcctc cagcagaatt tttattttct gagcatccag gtcaaccctc attactccct
      241 tgtaagcatg tgccaccaca tttacttcgg cttcgtacaa tgcaacggca atcctgcgga
      301 cgacctgggg gtcaatattc agttgcttga gtattttttt tatgtccgag ctcgcttttc
      361 cggctgaact aaaatcccca ccctgtatat cgtactcgaa ttgcatatca aaagatgggc
      421 tttatcccgt tgctgtacaa tatgcccgaa acttcaaaaa gcgtaagcgg tgttgtgata
      481 agcgaaatat tgttttcagc agcaagtgtt atcatttcat cggttacttt tttatcgcgg
      541 gcaaaaataa tacactcaat gttcgacatc tcggctgtcc gtaatgtctg aaccgtagat
      601 aaaccggtaa tgagtatcgt cttttccata ttaaagcgca acacatcact catcaggtcc
      661 gacgcaaaag cataagtaaa ttcttgtgac gtgctgcatt cctggcaaat ggtacctttc
      721 accaattcgt ttaccgtgta taaattcata ggattaaact ttaaaaactc ctgtcatttt
      781 gctcagagca cacatcagac cacctctgtt tttggtagaa aaaagattat tttgtcaaat
      841 agaaacttgc agcaataatt atcgtcaatt acaattactc tcataacaga ctggtaacca
      901 ataagctaat gcaatacgtc tggtttctat tacaaagtaa atcattttat tcaaataaat
      961 gaaattttca ggggtaaatt actgtgttga aacttaatac tccctcttca tataatgggt
     1021 gtgaagcaag tgatgcgact tctcccccag cggttttttt aagaagtcgg catatattgc
     1081 ggtaatctcc ggattttcat gtgattttcg tatcgcttta tgatcgtcat cgtaataaat
     1141 agattcagca cgtttttcac gtgtttccgc gttggtggga ataggttgtc caccaccgcc
     1201 aaggcaacct cccgggcatg tcatcacttc aataaacacg tagggtgaag tgccgttttt
     1261 aatctgttcc aacaaaattc cggcgttttt gagtgtatgg gcgacagcta ccttaataac
     1321 cgttccgttc aaatttacgt ccgcctcttt tattccttgg aaaccccgta cagaagtaaa
     1381 attgacgtca tgtagtgttt taccggtata cacctcataa gctgtcctca gtgccgcttc
     1441 catcacccct ccggtagcac caaatattac ggcggcaccg gttgattcac ccaacgggct
     1501 gtcgaaccgt tcttccggga gactggaaaa atttaccccc gactcactga acatacgtgc
     1561 cagctcgcga gtggtaagcg aaaaatccac atcaaaaaaa tgttcctcat ctttcagttt
     1621 aagtttctct ttccagtaat ggaatgctcc gtccatttca ggacgtttcg cctcatactt
     1681 cttggctgta caaggcatga tggaaaccac cacgatgctt ctcggatcga ctcccgtttt
     1741 ttcggcataa taggtttttg ccacagcgcc aaacatctgc tgcggtgatt tacaggtgga
     1801 caggtgtctc aacaaacccg gataaaaatc ctcgataaac ttgatccatc ccggcgaaca
     1861 ggaagtaatc atcggtaact ccccgcccgt cctgatgcgg tgaagcagct cattcccttc
     1921 ctcgataatg gtaaggtcgg cagcgaaatt agtatcgaac actttcgaga atccgagtcg
     1981 gcgtaatccg gcaaccattt gacctgtaac caggcttccg taaggcattc ccatagcctc
     2041 tcctattccg acacgcacgg ctggcgcggt ttgcaccaga acaatctttc cgggatcttg
     2101 taaggctgcc cagacttcgc ttaccgaact tctctccgtt atagctcctg tggggcagac
     2161 cagggcgcat tgcccgcaat ttgaacaggc tacaagcccc aaccccgaat cgagaaaagt
     2221 ggagattttg ctttttaatc cccgtcccga aaaatcgatt gcctttactc cctgcatctg
     2281 gctgcaaacg gcaacgcaac ggccgcacag gatgcatttt tccgggtcgc gcaccagcga
     2341 aagcgatgtt tcgtctttta ccagcagctc cttccgggtg cgaacaaacc gcctagcact
     2401 aatccctaat gcatgggtca actcctgcaa ttcgcagttt ccgttccggt cacaaatcaa
     2461 acaatcctga ggatgattgg ccagcaataa ctcaacattt acttttcttg cctgcatggc
     2521 cctcgggctg tttgtagaaa tttccattcc ctcggtaacc tgggtgatgc acgagcgtaa
     2581 taacgattta gcacctttca cttccaccac acaaacgcca cacgacgcat tttgcgatac
     2641 atctttcagg taacacagcg taggcacctc tattcccaca gaacggcatg cttccagaat
     2701 ggtggctcct tcagctacct gaacaggacg gttattgatg ttaatttcca taatacagct
     2761 ttttcagatt aatatttcac gtcacacctc aggcacctct ccacctcatt gcgggcctgc
     2821 tcacccgtaa atccgcagga gatctcgttg aaattattca cccggtcttt caccgaaagc
     2881 ctcctcggaa cattcttcga agcgggtttt ggatttgcgg gcacaacgtt tcgatagtca
     2941 aacgggggcg agagcaggca aaaccggttt tcacccatca acatcctgtc catcgcagca
     3001 gcggctttct tcgccatccc cattgcctct gccgctgttg aagggccgct taccgcatct
     3061 cccccggcgt agactttcgg atcagttgtt tgaaaagtga acggatcaat aacgattcgc
     3121 ccgtcccggg taagcttcat atttttttca gccagaaatc cggaatctac ccgttcaccg
     3181 acagccagca caactgcatc acacggaatt tcttcaaata ctccggtggc aaccggtttt
     3241 ttcctcccgg aagaatcgat gcttccaccc gacatccttt caatgtgcag cgcttttact
     3301 ctgcccgatt tatccgttat aatcccgttg ggagaagcca taaaacgata gttgattttt
     3361 tcttcttccg attccgatat ctcgacggcg ttggcaggca tatccccttt ttcacgccgg
     3421 taaacgatgg tcacatcctt tcccaggcgt aataaactcc gggccgtgtc gatggccacg
     3481 tttccggcac cgacaatagc aacccgctca cccactttcg gtgttttccc cacagccatc
     3541 tctttcagaa ttgttgttcc cgcaaaaaca ccttttgcat cttctcccgg aattttcaaa
     3601 tcaatatttt tccatgcacc tatcgccaaa taaatgacat cgaactcatt ttttaactct
     3661 tccagggaaa ggttgtctcc caatcgctgg ttgaacttga atttcacacc cagttttttt
     3721 atcagcctgg cctccttcct caacacttct ttcggcaaac gataatgagg tatgccgaaa
     3781 cgtgctactc ccccagcttc ggacaaagcc tcgtaaacgg tgatatcatg gccgaggcgt
     3841 accaggtaaa aggctgccgt aagccctgct ggcccggcac ctacaatggc aatccttttt
     3901 ccggtgggag gaagtttttc tttaatcagt ttatggtaga tcccgttctc tttccctagc
     3961 tggtacatgg tatcagccag gtaccggtgg atctcgcctt gcgaaaccgg ctggtccagc
     4021 atttcacgac ggcaacgcat ttggcaatgg aagtgacaaa tcctccctat tgttcccggc
     4081 aggggattat cgcgcagcgt aagctcaaag gcttcttcca cacggtcttc cttaatcagt
     4141 tcgatgtaac ccggaatgtt catgtgcagc ggacagctat tttcgcacaa agccatgaac
     4201 agcgactcgc atgtccctgc atgacaccgt ttctcccgga tatgctcttc gtactcgttc
     4261 cggaagtatt ttaacgtggt cagaatggga ttgggggcgg tttgcccgag agcgcaaagc
     4321 gacgaatctt tgataacacc gctgagcagc tccaagaccg ttaaatcggc gaacgttcct
     4381 tgtccccggg tgatgcgggt gagaatagcc aatgcttgtg acgccccttc acgacagggc
     4441 gtacattttc cgcacgattc tcctgcgttg aactccagaa aataccgcgc cacgtcaacc
     4501 atgcagttgt cctggtccat caccaccatt cctcccgaac ccatgatggc tccaatgcct
     4561 gccagtgact cgtaatccac cggggttgaa aaaaactcca acgggatgca accgccggaa
     4621 ggcccgccgg tctgcacggc ttttacgcgt ttggaagttc ctgttccctc cccgatattg
     4681 aacacaaatt gctccaacct tgacccgaga ggaagttcta ccaaccctgt attcttcact
     4741 tttcccacca gtgagaacac ttttgttcct gggctttttt ccgtccccat agctgtgaac
     4801 catccggcac ctttttcaat gatgaccggc acgttgcacc aggtttccac gttgttgata
     4861 ttggtgggtt ttccgtacaa tcctttgctg gcaggataag gcggacgcgg aagcggtcgg
     4921 cccgccttcc cttcgatgga agcaatcaat gctgtctcct caccgcaaac gaaggctccg
     4981 gccccttcaa caatgtccat cttgaaactg aaaccggtac ccaatatatt ttcacccatg
     5041 agcccgagtt gttcagcttg cccgatagcc gtccgaagcc gctttacggc aagcggatat
     5101 tctgccctca cgtatgcaat gccctcactt gctcccatgg cgtaagcacc tatcagcata
     5161 ccttcgagca acatgtgcgg gtcgctttca atttcgttac ggttcatgta cgcgcccgga
     5221 tcgccctcgt ccgcattgca gataacatat ttctgccggg catcctgttt ttgcatgatt
     5281 gaccatttta ctcccgtagg gaaacctgcc cctccccggc cacgcaactt cgacgccatc
     5341 acttgctcta ccacttctgc cggctcataa gccataaccg cttttgccag ggcattgtat
     5401 cctcccacag caatataatc gaaaatgtct tcagggtcga tgaggccggc atggcgcaac
     5461 accactttct tctgtccctt aaaaaagggt acctcatccc agtgaggaat atcttcgtaa
     5521 ccctttccaa ataccacctc cgaagtcagg aaatcccatt catcgatgcg acacaacagc
     5581 ttgtttttat acagcctgtt tttatgtaaa ttatcaatga tgcgttttac atccttcact
     5641 tccactttac tgtaaaccag cataggcttg cccggctgat agaacatcac catcggttct
     5701 tcagcgcaga aaccgaaaca accggcctgc ttgagtttac aatcgaagtt attctccgac
     5761 agcacctttt cgaaatgatg ataaaccgta gcggctccgt taccgatacc gcaggtcccc
     5821 attccaacca aaatcatggg agtcgccggc aggatccttt ccttgtgctg ttttcttaag
     5881 gtttcaattt cagacggttt catagccgtg gggtgtattt gctcaggatt ttcattaatt
     5941 tccgggaatt cacgttactg taaataacat tgtctatggc tacgacgggc gattgtgcgc
     6001 attgtccgaa acaggccact gtccttatcg taaacatgtt atcgggagta gtgaaagatg
     6061 attcagtatc gtcaccggta cgtttaatac ctaaaatttt tccgatatca tccagcaagg
     6121 cttttgaacc ttttgtgtga cacgctgtac cgcgacaaac gacaatcgaa tgcttgcctt
     6181 gaggttttaa attaaagaag gaataaaacg tggctacact gtagacctgt gtataaggca
     6241 ccttcagttt tttcgatatt tcggccagcg tttcacccgg aagatagttc aggggatttt
     6301 tctcctgtgc ttcttccaac gtggtgagca acataccaag ctttccgcgg tatttttcga
     6361 tgatttcatc gaaaatttcc cccgtaacgg gagcgtttat cagtgtttcc atacccgcat
     6421 tttttattaa acaaaatcaa cctttaagac ttgtaagaga ttaacagtca ttcgactaaa
     6481 cacacatttc atcttttaca aatgtagtgt attttctttg taattaaaga aatatttctt
     6541 taattattta cacctgtttt ctatttggaa taattctaca taaaacgccc gttgtctatt
     6601 gttaaataac agctggaata attacttcct gctacctcat gcggactacc ccacactgcc
     6661 ttttttcgaa agaaggaaaa ttatctgaaa atcaattttt tcatgccctg catcaacgaa
     6721 aaaagcgaca tcaaacggaa agtatgcgat atattttgta cttttgtgga gtttttgcga
     6781 aactcgaaaa aataaaacaa aatgcatcat ttagagctta gtgaacagga gataatccgc
     6841 agacaaagtt taaacgaatt aagaaattta ggaattgaac cctaccccgc agcgctttac
     6901 gaagtaaacg cttattccac cgatataaaa gaagaattca acgatgagga gaaacgcccg
     6961 gtaagcatag cagggcgcat catgaaccgc cgcattatgg gcaaagcgtc cttcatcgaa
     7021 ctacaagact caaagggaag aattcaggtc tacatcacac gcgacgatat ctgtcccgga
     7081 gaagacaaag aactttataa tactgttttc aaaaaactgc tcgatattgg cgatttcatc
     7141 ggaattaccg gatttgtttt ccgcacccag atgggcgaaa tttctgtgca tgccgaatcg
     7201 ctgaccgtgc tggccaaatc gctgaagccg cttcctgtag tgaagatgaa agacggcgtg
     7261 gcctacgaca agttcaacga ccccgaactt cgttaccgtc aacgctacgt ggatctggtg
     7321 gtaaacgacg aagtaaaaga tattttcatc aagcgcaccc gtatttttaa tgccatgcgc
     7381 gagttcatca acaaatttgg ctacctggaa gtggatactc ccgttttgca gagtatcccc
     7441 ggtggtgccg ccgcccgccc ctttatcacg caccacaatg ctctggatat tgatttgtat
     7501 ttacgcattg ccaacgagct ttacctgaaa cggttgatcg taggtggctt tgaaggtgtt
     7561 tacgagtttt cacgagactt ccgcaatgaa ggaatggacc ggacgcacaa cccggagttt
     7621 acggtaatgg agatctacgt ctcctacaag gattacaagt ggatgatgga atttaccgaa
     7681 caaatgctgg aacagatcgc cacgaaggtg ctgggaacaa caaagacaac catcggcgat
     7741 agggaaatcg attttaaagc gccatatcca cgcgtcacca tgatcgacgc cattaaggaa
     7801 cataccggaa tggatatcac cggaatggat gaagcacaat tgcgcgaagt ttgcaggaag
     7861 ctgaacatcg aggtagacga aaccatgggt aaaggaaaac tgatcgatga aatcttcggc
     7921 gagaagtgcg aggggaatta cattcaaccg acattcatca tcgactatcc catcgaaatg
     7981 tcccccttgt gtaaacgcca ccgggctaat ccggagctta ccgaacgctt cgaactgatg
     8041 gtgaacggta aagaactggc aaacgcctac tcggagttga acgatccgat tgaccagcgc
     8101 gaacgctttg aagaacagct ccgcctgtcg gaaaagggcg acgacgaagc catgtttatc
     8161 gaccaggatt ttctgcgcgc cctggaatac ggcatgccac ctacatcggg gatgggtata
     8221 ggtatggaca ggcttacgat gctgctcacc ggacaaacca ccattcagga agtactcctt
     8281 ttcccgcaaa tgcgtcccga gaaaaaaacc aagaaggatt caacgtccaa atatgttgaa
     8341 accggaatcc cggaagcgtg ggttgctgta ttgcagaaag cgggttacaa tgaagtgaag
     8401 agccttacag acgtaaaccc caacaagctg caccaggaaa tttgcgggtt gaacaagaaa
     8461 ttcaagatgg agttgaacaa cccgtcggtg gaagatgtta aatcgtgggt ggagaacgca
     8521 aaaaaaaacg aatcacttaa cgcacaatga gttcactcgg aaaaattgct attcttggcg
     8581 gtggcacgtg ggctacggct ctggcaaaaa tcgtgctgat gaatgaaaag cacatcaatt
     8641 ggtttatccg tcgcgacgat cagattgaag gtttttacaa actcggccgc aaccccagtt
     8701 acctgacaaa cgtaaaattc aacctggccc agattacgtt ctattcgaac atagaaaaag
     8761 caatcaaaga ctctgacacc atcatcattg ctatcccctc tccctatgtg aagcaatata
     8821 tgcgaaaaat atggatgagc agtacactaa aggggaaatt tgtggtctct gccgtcaaag
     8881 gcatgatacc gaacgacaat attgtggtaa gcgaatacct gatgagtaat tttaaaatcc
     8941 cgcgggaaaa catcggggtg atatccggcc cctgtcacgc cgaggaggtc gcgcttgaac
     9001 gggacccgtc ctgcgaagta tgcggcggga agaat
//
LOCUS       DAOSDR010000076         8787 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrimonas sp. isolate AMR_MDS_1826
            NODE_2961_length_8787_cov_4.457284, whole genome shotgun sequence.
ACCESSION   DAOSDR010000076 DAOSDR010000000
VERSION     DAOSDR010000076.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863355
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrimonas sp. (wastewater metagenome)
  ORGANISM  Petrimonas sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Dysgonomonadaceae; Petrimonas.
REFERENCE   1  (bases 1 to 8787)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8787)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:10:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,720
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 35
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8787
                     /organism="Petrimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2961_length_8787_cov_4.457284"
                     /isolate="AMR_MDS_1826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2023866"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(27..1634)
                     /locus_tag="PLI30_04480"
     CDS             complement(27..1634)
                     /locus_tag="PLI30_04480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005776499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HOI78845.1"
                     /translation="MKITKLTIISGVMLLIIGATHTSCSDALDLAPIDYYGSGSYWKT
                     EAHATGYIDGIHKHLRDAAWQHTITFGELRGGHYISGTSGDGSSVYNGSIIQQNFDAN
                     NTGVSKFGDLYGRITNCNLFIARVTDADYIAEAKKNYYLGMVYGIRAFYYFDLYRIYG
                     GVPLRLGVEVVDGELDPTKLYMPRAKASEVMAQIKSDLQKSLDYFGSVNDFNPYGMGK
                     KVYWNKAATESLIGEVYLWNAKVTTGDNTANQADLAVAKQHLENVANNYGLKMLTEFP
                     RVFDATNKANDEIIFAIRYAEGEASNAVNQYIYNVGTGQTNKNAYLEDGTLFDDPLQI
                     ATSSSQQRYEYTKQFFEIFDKKDSRRNATFLPSYNKDSDGNLIYRGVSTKKNIGYINS
                     NGIRQWVGDFVFYRLPWVYLSLAEIANMEGDNTKVEEYINKVRQRAYGANWSSEFTFT
                     ASDFTTNELAILREKDKEFVQEGQRWWDLRRMTLTKGGKHLVFTQEGSVDGNPVLNEA
                     TETHKVLWPLDKSLLDNDDALEQTPGY"
     gene            complement(1657..4935)
                     /locus_tag="PLI30_04485"
     CDS             complement(1657..4935)
                     /locus_tag="PLI30_04485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008760185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOI78846.1"
                     /translation="MKSWIVSLSFLYLSTFSLMAQNITVSGKVTDNVLNEPLIGVTII
                     VQGTSNGTVTDIGGNYTLANVPSTGNLEFSYVGMQTQVIAVNNRTSINVTLREDTELL
                     EEVVVTGYGGTQLRSKVTNSIAKVSNETLTVGVFSNPAQALSGAVSGLRVIQTSGNPG
                     ATPQIVLRGGTNLDGSGSPLVMVDGQLRDGLNDINPEDIESMEVLKDAGATALYGARA
                     SNGVILVTTKSGKAGQRSLNFKAKLGLNYVNNPYTFLGAEDYITYMRKAYQNTPWASK
                     GNLTGSTPLGTGNVYGENMLWNLMKQTDDNKFLLEKGWKSMPDPINPTDMLIYRETDI
                     AKYSFVDPSFTQDYNLNMSGGNDRGNYYAGLGYNHSEGLPITSYYKRYSFILNGSYKV
                     TDWLKTNSSFNYNRANWQSMPPTQTSEANYFGRIQSTPPTARYEDEEGKMLLGANSGD
                     GNQSYQANRFMRDNQTDKFTMIQSAEAKLLKNLTLRTSAQWYYSEGFYESFNRDYETN
                     PGKWNRTRSTSAEFDRDFSQTYNATLNYNRLFAGKHNIDALLGAEYFDKGNKGFNASG
                     SGAPTDDFWDLGLTDTGEGKRNIDSWHSQYRILSYFGRANYDYQGKYLLSGVFRYDGY
                     SSLLGDNRWGFFPGVSAGWIFGNEDFVKENLPVLSFGKLRSSFGVNGNASGIGAYTLQ
                     GSYSPQAYNGRTGFLIGTLPNPGLRWEKTRTFEVGTDVSFFENRLNANLTYYSRLTMD
                     KYAAFALPSTTGFSSITNNNGEFRNRGLELELSGRIIRTEDFSWEMKGNITYNKNKII
                     SLPANGLERNRQGGIEIYTGKKVINPETGLEEDGKIFVGGYQEGQEPGLLVGYQFNGI
                     YKSVDEIPGNLVVKTGNDQGKYQYGPAAYAALTEAQKRNAILIEPGDAKWEDINGDGV
                     IDSFDRIVIGNTTPRWTGGFNTTFKYKNFMLYGRFDFGLGFYTYDNTTPWFLGAMQGT
                     YNTTTDVFDTWSPENPNAKYPRYVWADQLGTGNVNRPSTLFAYKGDYLAFREISLAYS
                     LPQNIAKKLYMQAMNVSITGQNLGYLTAAPIASPERAIGAGVASGTGYGLPRTLLFGI
                     NLTF"
     gene            complement(5001..6623)
                     /locus_tag="PLI30_04490"
     CDS             complement(5001..6623)
                     /locus_tag="PLI30_04490"
                     /EC_number="3.2.1.18"
                     /inference="COORDINATES: protein motif:HMM:NF024488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exo-alpha-sialidase"
                     /protein_id="HOI78847.1"
                     /translation="MRKKIAVRLSVFLFAPFLLNSFSGCNSSTGPATEITGVEMTENT
                     EYTGRGNQNEVLIALKVNVKENETISLKNIVLVKNAATQPSDLKEIKIYSTGSLDTLN
                     TRTLEQATLLGKAPLSKKSTKIRLSGDLQPGMNHLWITADIADNATEGNKIGVSLTSV
                     GYNKNKKFEHQPEPSHGREILLARKLIFSPGDFDSKFYRIPAITTAEDGSLITATDKR
                     KENIWDLPQDIDVVIRRSTDNGKTWSEPLTIAEGKGLAKGYGDPALIKLNNGKILCVY
                     AGMNGLWQSRPDSLIRTYVSESADNGITWTPVRDITAQLYGVDCPDPVRKKWMASFCA
                     SGQAIETSSGRVMLVAAVREGSDYSLNNYLFYSDDEGITWNVSQKAMEGGDEAKVVEL
                     ADGSILMSIRNKNKGNRYYTRSTDNGITWTSVQLWKDLKDPGCNGDMIRYSMEKNGAG
                     KNILLHSIPFDEKDRKNVSVYVSYDEGKTWPAGKTICAGPSAYSSLTVLEDGTIGAYV
                     EEGEPGMNLVYLNFSLEWLLRNTDEMNPGKTK"
     gene            complement(6658..7851)
                     /locus_tag="PLI30_04495"
     CDS             complement(6658..7851)
                     /locus_tag="PLI30_04495"
                     /EC_number="5.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016662049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AGE family epimerase/isomerase"
                     /protein_id="HOI78848.1"
                     /translation="MDTKISVINIEEKILQYKNELLDNVLPFWQSKSQDYEFGGYFTC
                     FDREGNVFDTDKFVWLQSRQVWMFSMLYNNVEKKQEWLDCAIQCGEFLKKYGHDGNLN
                     WYFSLTREGEPLTEPYNIFSYTFATMAFGQLSKATGNAEYAEIAKRTFDIVLSRRSNP
                     KGKWNKAYAGTRNLKSFSLPMILCNLALEIEHLLDPEFLKKTIDECIHEVMEVFLRPE
                     LGGLIVENVNEDGSLSDTFDGRLVNPGHSIEAMWFIMDLGERLNRPELINHAVETTLR
                     MLNYGWDKQYGGIFYFMDRKGLPPQQLEWDQKLWWVHIETLISLIKGYQLTGSPECLA
                     WFEKVHDYTWKHFRDPEHPEWWGYLNRQGEVLLDLKGGKWKGCFHVPRGLYQVWKTME
                     KIDKI"
     gene            complement(8055..>8787)
                     /locus_tag="PLI30_04500"
     CDS             complement(8055..>8787)
                     /locus_tag="PLI30_04500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005646109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HOI78849.1"
                     /translation="KAMIFAAGLGTRLKPLTDTMPKALIPVAGKPLLWHTIQKLKAAG
                     FDEIIINVHHFAGQIRQYIQDNNRFGIRIEFSDETQALLDTGGGIKKASWFFDDEKPF
                     LIHNVDILSDIDLQNLYHFHTNSNSVATLVVSERKTSRYLLFDKNNHLAGWINEKSGE
                     TKSPFSDFNADHYKKLAFSGIHILHPSILSYMQDFPDKFSIIDFYLFACNILPITGYT
                     PASLKIIDVGKIDSLKKAGKLGYRG"
BASE COUNT         2127 a         2003 c         1943 g         2714 t
ORIGIN      
        1 tggacctgag ttaatatatg aaaagattag tatccgggtg tttgctccag ggcatcatca
       61 ttgtcgagga gactcttgtc tagcggccac aatactttgt gtgtttctgt ggcttcattt
      121 agaaccgggt tgccgtcgac acttccttcc tgggtgaaaa ccaagtgttt tcctccttta
      181 gtgagggtca ttctgcgtaa atcccaccag cgttgacctt cctgtacgaa ttctttgtct
      241 ttctcccgca ggatggctaa ttcgtttgta gtgaaatcac ttgccgtgaa ggtaaactca
      301 ctactccagt ttgcgccgta tgcccgctgg cgtactttat tgatgtactc ttccaccttt
      361 gtattgtctc cttccatatt ggcaatctct gccagggaca ggtaaaccca ggggaggcgg
      421 tagaacacga aatcgcctac ccattgacgg atgccgttcg agttgatgta tcctatgttc
      481 tttttcgtgg aaactcccct ataaataaga tttccatcgc tgtctttatt ataagagggt
      541 aggaaagtag catttcgtcg tgagtctttt ttgtcgaata tctcaaaaaa ctgtttggtg
      601 tattcgtagc gttgttggga agaggaggtt gcaatctgta aagggtcatc aaaaagggtg
      661 ccatcttcca gataagcatt tttattggtt tgtccggtac ccacattata gatgtactgg
      721 ttgactgcgt tggaagcttc tccttctgca taccgtatgg caaaaataat ttcgtcattg
      781 gctttattgg tagcatcgaa taccctcgga aactccgtca gcatttttaa tccgtagtta
      841 ttggccacat tttccagatg ttgttttgct acagccagat cagcctggtt cgctgtgttg
      901 tctccggttg tcactttcgc attccagaga taaacctccc cgatcagcga ttctgtggca
      961 gccttgttcc agtaaacttt cttgcccatg ccgtacggat tgaaatcatt cacactgcca
     1021 aaataatcga gtgatttctg taaatcgctt ttgatttggg ccattacttc tgaagctttt
     1081 gcacggggca tatagagctt ggtcggatcc agttccccat caacaacctc tactcccagt
     1141 ctcagaggaa ctcctccgta gatgcggtac agatcaaaat aatagaaagc gcgtatcccg
     1201 tataccattc ccagataata attcttcttt gcttcagcga tatagtcagc atcggttact
     1261 cgtgcaataa acaggttgca attggtgatt ctgccgtaca ggtccccaaa tttagatacg
     1321 ccggtattgt tagcatcgaa attttgctga ataatggaac cgttgtacac cgaagagccg
     1381 tctccgctgg tacccgaaat ataatgtcct cctctaagtt ctccaaaagt aattgtgtgt
     1441 tgccaggctg catccctcag atgcttgtga atgccgtcaa tgtatcccgt agcatgagct
     1501 tcggttttcc agtaggaacc gctaccataa taatcaatgg gagccagatc cagtgcgtca
     1561 ctgcatgaag tgtgggtcgc tccgatgatc agaagcatca cacctgatat gattgttaat
     1621 tttgtaatct tcataatgtt ggatttctaa agatgtttaa aatgttaagt taataccaaa
     1681 taacagggtc cgtggtaggc cgtaaccagt tccgcttgct acccctgcac caatggctcg
     1741 ttcgggactg gcaatgggtg ctgcggttag ataacccagg ttctggccag taatggatac
     1801 attcatcgcc tgcatataga gcttttttgc tatgttttgc ggtaacgaat aagccaggga
     1861 aatctctcga aaagccaggt agtcaccttt ataagcgaaa agagtggaag gccggtttac
     1921 gttacctgtt ccaagttgat cggcccatac atagcgtgga tatttagcgt tcgggttctc
     1981 cggcgaccaa gtgtcaaata catctgtagt tgtgttgtac gttccctgca tagctcccag
     2041 gaaccaggga gtagtgttgt catacgtata gaagcccaaa ccgaaatcaa aacggccata
     2101 caacataaag tttttatatt taaaagtggt gttaaatcca ccggtccagc gtggggttgt
     2161 atttccgatt acaatccgat cgaagctatc gatcacgccg tctccattga tgtcttccca
     2221 tttggcatct cccggttcaa ttaaaatggc gtttcttttt tgtgcttctg tcaaagcagc
     2281 gtaagccgca ggtccgtact gatattttcc ctggtcgttt ccagtcttta ccacgaggtt
     2341 tcctggaatt tcatccacac ttttgtaaat accgttaaat tgataaccga ccaagagacc
     2401 tggttcctgt ccttcctgat aaccgccaac gaagatcttt ccatcttcct ccaatcctgt
     2461 ttccggatta attacttttt tgccggtgta gatctctatc ccgccttgtc ggtttctttc
     2521 caaaccgttg gcgggcagcg agataatttt gtttttgttg taggtgatat ttcctttcat
     2581 ctcccagctg aagtcctctg tccgaatgat cctgcctgac aactccaatt ccaatcccct
     2641 gtttctgaat tcgccgttat tattggtgat agaagagaaa cccgtggtcg agggtaaggc
     2701 aaaggctgca tatttgtcca tggtaagacg gctgtagtag gttagattgg cgtttagtct
     2761 gttttcaaag aagctaacgt ctgttcctac ctcaaaggtg cgtgtttttt cccatctcag
     2821 tccgggattt ggcaatgttc caatcagaaa accggtgcgt ccgttgtatg cctgtgggct
     2881 ataagatcct tgaagagtat aagctccgat accgcttgca ttaccgttta caccaaaact
     2941 ggaacgtaat ttcccaaagg agagtacggg caggttctct tttacaaaat cttcattgcc
     3001 gaaaatccat ccggcagata cgcccggaaa aaatccccac cggttatcac ctaacaggga
     3061 tgagtaacca tcgtaccgga atacgccgga aagcaggtat tttccttggt agtcataatt
     3121 tgcacggcca aaataggaga gaatacggta ttgtgaatgc caggaatcta tgttgcgttt
     3181 gccttctcca gtgtcggtca gtcccaaatc ccagaagtca tccgtggggg ctccagaacc
     3241 ggaagcattg aatcctttgt ttcctttgtc gaaatattcg gctcccagta gcgcatcaat
     3301 gttgtgcttc cctgcaaata accggttgta gtttaacgtg gcgttgtatg tttgagaaaa
     3361 atcacgatcg aattcagcag atgtactgcg ggttctgttc catttacccg gattggtctc
     3421 ataatcccgg ttgaatgatt cgtaaaatcc ttcgctgtaa taccattgtg cagaagtcct
     3481 taatgtcagg ttcttgagta gttttgcctc tgccgattgg atcatggtaa atttatcggt
     3541 ctgattgtct ctcatgaacc tgtttgcctg gtatgactgg tttccgtcgc cggagtttgc
     3601 acccagtaac atcttgcctt cttcatcttc ataacgtgct gtaggagggg tagactgaat
     3661 acgtccgaaa tagttggctt cactggtctg cgtgggcggc atggattgcc agttggcgcg
     3721 gttgtagtta aaactcgagt tggttttcaa ccagtccgtt accttataac ttccatttag
     3781 gataaagctg tagcgtttgt aatacgaggt gatcggcagt ccttccgagt gattgtaacc
     3841 caatcctgca tagtaatttc ccctgtcgtt acctcctgac atattcaggt tgtagtcctg
     3901 ggtaaatgag gggtccacaa aactgtattt agctatgtcg gtttcacggt aaatgagcat
     3961 atctgtggga ttgattggat caggcattga cttccatcct ttctccagta aaaacttatt
     4021 gtcatccgtt tgtttcatca aattccaaag catattttcg ccgtacacgt tccctgttcc
     4081 tagtggggtt gatcctgtca gatttccttt tgatgcccag ggcgtatttt gataggcttt
     4141 tctcatgtag gtgatataat cttctgcgcc caggaaagta taggggttat tcacatagtt
     4201 gaggcctaat ttggctttga agttaagaga tctttgcccg gccttcccac ttttggtggt
     4261 aacaagaatt acaccattac tggcccgtgc cccgtacagg gcagttgcgc ccgcatcttt
     4321 cagtacctcc atggactcga tatcctccgg gttgatgtcg tttagtccgt cgcgcagctg
     4381 tccatcgacc attaccagcg gtgagccgga tccatcaagg ttggtgcctc cacgaagtac
     4441 tatttgcggg gttgcacccg gattacccga tgtctggatt accctcagtc ccgataccgc
     4501 acccgacaat gcttgtgcgg gatttgaaaa tacccccacg gtgagtgttt cgttcgaaac
     4561 tttggcaatc gagttggtta ccttgctcct caactgtgtg ccgccgtaac cggttactac
     4621 cacttcttcc aaaagttccg tatcttcccg caacgttaca tttattgaag ttcggttgtt
     4681 aaccgcaata acttgtgttt gcattcccac ataagaaaac tccagattac cggttgacgg
     4741 tacattggcc aatgtatagt tgccgccgat atcggtaacc gtcccgtttg aagttccctg
     4801 tacaatgatc gtcacgccta taagcggctc attgagcaca ttgtcggtaa cttttccact
     4861 cactgtgatg ttttgtgcca tcagcgagaa agtggagagg tacagaaagg agaggctgac
     4921 tatccaactc ttcattgatt tagcgatttt agaatttttc atacaatctc caattaaaaa
     4981 aattaatact tttttgattt ctatttagtt tttcctgggt tcatttcatc tgtgttccgg
     5041 agaagccact cgagggaaaa attcaggtaa accaggttca tacccggctc cccttcttcc
     5101 acgtaggctc ctattgttcc gtcttccaat accgtaaggg aggaataggc agaaggtcct
     5161 gcgcagattg tttttccagc cggccacgtt tttccttcgt cgtaacttac gtaaaccgaa
     5221 acattcttgc ggtctttttc atcaaatggg atggagtgca gcaatatgtt cttgcctgct
     5281 ccattctttt ccatcgaata gcggatcata tcgccgttgc agcctggatc tttcaagtct
     5341 ttccacaatt gtacggatgt ccatgtgata ccgttgtcgg ttgaccgggt gtaatacctg
     5401 tttcctttgt ttttgttgcg aatactcatc aggatagacc cgtcggccag ctctacgact
     5461 tttgcctcat cgcctccttc catggctttt tgcgaaacat tccaggtaat accttcgtcg
     5521 tctgaataaa acaaatagtt gttgagcgag taatcgctac cctcgcgaac ggcggcaacc
     5581 agcataacgc gtcccgagga tgtctcaatg gcttgtccag atgcacaaaa cgaggccatc
     5641 catttttttc ttacgggatc cgggcaatct acgccgtaca gttgggctgt aatgtcgcgg
     5701 acgggagtcc acgtgatacc attatcggca ctttcgctca cgtaggtacg gatcagcgaa
     5761 tcgggtctgg actgccacaa tccgttcatc cctgcataca cgcataggat tttgccatta
     5821 ttgagtttga tgagtgcggg gtctccgtat cctttggcca atcctttacc ttcggcaatg
     5881 gtaagcggct cgctccatgt ttttccgttg tcggtgctgc ggcggataac cacgtcaata
     5941 tcctgcggca aatcccagat attttctttt cgtttatctg tggcggtaat aagcgaaccg
     6001 tcttctgctg tagtgatagc cggaatccgg taaaatttgg agtcgaaatc accaggagag
     6061 aaaatcaact tgcgtgctaa caggatttct cttccgtgag aaggttccgg ctgatgttcg
     6121 aattttttgt ttttattgta tccgacagac gtgagcgaaa cgccaatctt atttccttcg
     6181 gtagcgttat cggcaatatc ggcggttatc cacaaatgat tcatacccgg ctgcaggtct
     6241 cccgacaacc ggattttggt gctcttttta gataacggtg ctttgcctag cagggttgct
     6301 tgttcaaggg ttctcgtgtt gagcgtatcg agtgagccag tagagtaaat ttttatttcc
     6361 ttcaggtcgg agggctgcgt agcggcgttt ttgacgagaa cgatattttt cagggagatg
     6421 gtttcgtttt cctttacgtt aaccttcaac gcaattaata cctcattctg gtttcctctc
     6481 ccggtatatt cggtgttttc agtcatttcc acacctgtta tttccgttgc aggaccggta
     6541 gaactattgc atccgctaaa gctgttcagc aggaatggtg cgaacagaaa gacggataat
     6601 cttacagcga ttttttttct cattgttgtc tattaatggg taatacattt aaaaaattca
     6661 gattttatca atcttttcca tcgttttcca tacctgatat aagccccggg gaacatgaaa
     6721 acaacctttc cattttccgc ccttcaaatc gagaagcacc tcaccctggc gattgagata
     6781 accccaccat tcaggatgct ccggatctct gaaatgcttc caggtgtagt cgtgcacttt
     6841 ttcaaaccat gccaggcatt ctggcgaccc cgttagctga taacccttga tgagtgatat
     6901 aagtgtttcg atatgcaccc accagagttt ctggtcccat tccagctgtt ggggaggaag
     6961 tcctttacgg tccataaaat aaaagatacc tccgtattgt ttgtcccaac cgtagttaag
     7021 cattcttaag gttgtttcta ctgcatggtt gatgagttcg ggacggttga ggcgttctcc
     7081 caaatccata ataaaccaca tggcttcaat gctgtgtcca gggttgacaa gccgtccgtc
     7141 aaatgtatcg gaaagtgagc cgtcttcgtt tacgttttcc acgatcagcc ctcccagttc
     7201 ggggcgtaaa aagacctcca taacctcgtg aatgcactcg tctatggttt tttttagaaa
     7261 ttcgggatcg agcaaatgtt ctatttcgag tgcaaggttg cagagaatca ttggcaaaga
     7321 aaacgatttc agattgcgtg ttccggcgta agctttgttc cattttcctt tggggttact
     7381 gcgcctggag agtacaatat cgaatgttct tttggctatt tcagcatatt ctgcgtttcc
     7441 ggttgctttg cttagctgtc cgaaagccat cgtggcgaaa gtgtacgaaa aaatattgta
     7501 gggctctgtc aacggttctc cttcgcgcgt gagcgagaaa taccagttga gatttccgtc
     7561 gtgtccgtac tttttcagaa attcgccgca ctgtatggca caatcgagcc attcctgctt
     7621 tttttccaca ttattgtata acatagaaaa catccaaacc tggcggctct gcagccatac
     7681 aaatttatcg gtatcgaaca catttccttc ccggtcgaag caagtgaagt atccgccgaa
     7741 ctcgtaatct tgcgatttac tctgccagaa gggaagcaca ttgtccaaca gctcgttttt
     7801 gtactgtagt attttttctt caatatttat gaccgatatc ttagtatcca ttttttccat
     7861 ttcttacccg ttatataaga ttaattaatt aatatattgt gcaaaataaa taaaaataaa
     7921 ttgataaaaa gaatataata ataaattaat ttaataaata tgttgcaaaa cataatatga
     7981 tcaaaataat gatactcttt atgaaaatat ttataaaatg gttgaaatgt tgaacaggaa
     8041 gatttgcttg ggtcctatcc ccggtatccc agcttacccg ctttcttcaa ggaatctatc
     8101 ttgccaacat ctattatctt taggctggca ggggtatatc ctgttatagg gagaatatta
     8161 caggcaaaca aatagaaatc gataatggaa aatttgtccg ggaaatcttg catatagcta
     8221 aggatggagg ggtgtaggat atggattccc gagaatgcaa gtttcttgta gtggtcagca
     8281 ttgaaatcag aaaatggtga ttttgtttct cctgactttt catttatcca tccggctaaa
     8341 tgattgtttt tgtcgaaaag gagataccgc gatgttttcc gttcgctcac gacaagcgta
     8401 gcaacagaat tgctattggt gtgaaaatgg taaaggtttt gtaaatcgat atccgaaaga
     8461 atgtccacgt tgtgaattaa aaacggcttt tcatcgtcga aaaaccacga ggcctttttg
     8521 atgccgcctc cggtatcgag aagcgcctgg gtttcatcgg agaactcgat acgaatgccg
     8581 aaacggttgt tatcctgtat atattgcctg atttgtcctg caaaatgatg cacattaatg
     8641 atgatttcat caaagcccgc tgctttcaat ttctgtatcg tatgccacaa caacggtttt
     8701 cccgctacgg gaatcaacgc tttgggcatg gtgtccgtca ggggcttaag cctcgtgccg
     8761 agtccggcag cgaaaatcat tgctttc
//
LOCUS       DAOSDR010000077         8714 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrimonas sp. isolate AMR_MDS_1826
            NODE_3004_length_8714_cov_4.409747, whole genome shotgun sequence.
ACCESSION   DAOSDR010000077 DAOSDR010000000
VERSION     DAOSDR010000077.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863355
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrimonas sp. (wastewater metagenome)
  ORGANISM  Petrimonas sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Dysgonomonadaceae; Petrimonas.
REFERENCE   1  (bases 1 to 8714)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8714)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:10:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,720
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 35
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8714
                     /organism="Petrimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3004_length_8714_cov_4.409747"
                     /isolate="AMR_MDS_1826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2023866"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1090
                     /locus_tag="PLI30_04505"
     CDS             <1..1090
                     /locus_tag="PLI30_04505"
                     /inference="COORDINATES: protein motif:HMM:NF024152.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SusD/RagB family nutrient-binding outer membrane
                     lipoprotein"
                     /protein_id="HOI78850.1"
                     /translation="SNTLNNLMINLGSVKSADMLDASYASYIKPKGYMGKRFQNQFST
                     YTNDPSIGFFFDGLHNTIDPRAYKLYAIPGDFTSPDAQFTSEDDLGPLERSVFADKDQ
                     NNEIATVNLAFTWNTMPGGSWGDMSAMNQIIGSLYNPILVKKYRNHSQKRVFFASWET
                     YFLLAEAALRGWTTPTSAKEAYEKGIKASLDYHGVSSFYDTYIASTDYNRVGTSVKWD
                     HTAEPPATVEVDIIDGYTNQAAKFAYKFPVASQTSYKKALNDQMTKVITQKFIAQNPW
                     LPLETWNDYRRLGLPFFENMVVENPLTNLPAITKDNVKTTQQPDFFPQRLKYPASLEN
                     SNPEGYKQAVELLGGTDAVLTPLWWARH"
     gene            1108..1248
                     /locus_tag="PLI30_04510"
     CDS             1108..1248
                     /locus_tag="PLI30_04510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI78851.1"
                     /translation="MKERLPPKRQPLFNAQMEEVTEPGEFTIMIGAASSDIRLRGSFL
                     CP"
     gene            1379..>2224
                     /locus_tag="PLI30_04515"
     CDS             1379..>2224
                     /locus_tag="PLI30_04515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005467514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HOI78852.1"
                     /translation="MSFLTNEKLTIVGAAGMIGSNMAQTAAMMRLTPNICLYDPFALG
                     LEGVYEEMRHCGFEGVDFTFTTDIREAFTGTKYMISSGGAPRKDGMTREDLLKGNAEI
                     AAQLGKDIKAYCPDLKHLTVIFNPADITGLVALIHSGLKPGCVTTLAGLDSTRLQSEL
                     AKHFGVKQSQVTNTRTYGGHGEQMAVFASTAKVDGKPLTDLIGTPELTNEDWDALKLR
                     VTKGGANIIKLRGRSSFQSPSYVSVEMIRAAMGGEKFGWPSGCYVNTVDFPNIMMAME
                     TNITKD"
     assembly_gap    2225..2234
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2235..2391
                     /locus_tag="PLI30_04520"
     CDS             <2235..2391
                     /locus_tag="PLI30_04520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004342460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HOI78853.1"
                     /translation="DGVVINETKGTDSEMTALKESYQHLAKLRDEVIAMGIIPATDQW
                     KSVNPNL"
     gene            complement(2467..4671)
                     /locus_tag="PLI30_04525"
     CDS             complement(2467..4671)
                     /locus_tag="PLI30_04525"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019537955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-translocating pyrophosphatase"
                     /protein_id="HOI78854.1"
                     /translation="MNYFYLVPIASLFALSFAFYFFKKMMRESEGTEQMKTIAKYVRE
                     GAMSYLKQQYKVVAIVFVILASVFAVMAYFGLQNNWVPFAFLTGGFFSALAGFFGMKT
                     ATYASARTANAAKNSLNKGLQVAFRSGAVMGLVVVGLALLDISAWYLVLDHFIDSAEP
                     GHKLIMITTTMLTFGMGASTQALFARVGGGIYTKAADVGADLVGKVEAGIPEDDPRNP
                     ATIADNVGDNVGDVAGMGADLYESYCGSILATAALGASAFVLNPAMQAKAVFAPMIIA
                     AIGVFLSILGIFLVHTGENADMKKLMAALNRGVNVSAVLIALFTFVILYLLGFENWLG
                     LSFSVISGLLAGIVIGQGTEYYTSHSFRPTQQIAESGKTGPATVIISGMGTGMISTFI
                     PVITIALAILFSYLCATGFDVQNMMSGENLSMGLYGIGIAAVGMLSTLGITLATDAYG
                     PIADNAGGNAEMCGLGKEVRKRTDALDALGNTTAATGKGFAIGSAALTALALLASYIE
                     EIKIGMLRLGQTVLEFADGSDAVEIAKAGFADFMNYYQVTLMNPKVLVGIFIGSMMAF
                     LFCGLTMNAVGRAAQKMVEEVRRQFREIAGILEGTGTPDYAKCVEISTKGAQREMLLP
                     SLLAIAIPIVTGVILGVPGVMGLLAGGLGSGFVLAVFMANSGGAWDNAKKYVEEGNLG
                     GKGSDVHKATVVGDTVGDPFKDTSGPSLNILIKLMSMISIVMAGLTVAWSLL"
     gene            4950..6155
                     /locus_tag="PLI30_04530"
     CDS             4950..6155
                     /locus_tag="PLI30_04530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019537957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family transcriptional regulator"
                     /protein_id="HOI78855.1"
                     /translation="MEKSFFLSTDKSPKNIQIKKEIINHFVNNGNDTIAELSKGLDLS
                     VPTVTKLIGELSDKGLVADFGKIQTAGGRYPNVYGLNPSSVYFIGVDMTRHHLNIALM
                     DFKGDIVKYEMGISYMYENTTDSFDVLCEHIQKFIRETGELKQKIYNICLNISGRVNP
                     ESGYSYSSFYFSEEPISNILSNRLNYKVTVDNDTRSMAYGEFMKGAVQSESNVIFVNV
                     SWGLGIGIIIDKKLYYGKSGFSGEFGHFPIFDNEIICHCGKKGCLETEASGLAIHRMV
                     LERINKGESSILTQQVKDLNKLTLTDIVKATNMEDPLCIEIMEEVGFKLGKYVAGLIN
                     IFNPELVVIGGQVAETEGFIMLPIRSAVRKHSLGLVNKDTQIIPSKLKNKAGVVGACM
                     IARSRLFDN"
     gene            6361..7659
                     /locus_tag="PLI30_04535"
     CDS             6361..7659
                     /locus_tag="PLI30_04535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006843939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylhomoserine
                     aminocarboxypropyltransferase/cysteine synthase"
                     /protein_id="HOI78856.1"
                     /translation="MRNLHFETLQIHAGQQPDQIEGSRVAPIHQTTAFVFKNAEHGAD
                     LFGLREFGNIYTRLQNPTTDVFEKRMAALEKGTAAVATASGQSAEFITINSLAGAGDN
                     IVSTSFLYGGTYNLFKVSFARLGVEFRFAKGDDVDSIESLIDEKTKAIYLEMIGNPEY
                     NIPDVEKIVDLARKKDLPVIMDNTFAQGGYLFNPIDWGVNIVLHSATKWIGGHGTSMG
                     GIIIDGGNFNWNNGKYPTLSEPSEGYHGLNFWEVFGDQSPFGNIAFAIRCRVEGLRDF
                     GPAISPFNSFMMLQGLETLSLRAERINASTLEIARWLEKHPKVEKVNYPGLESSKYNA
                     LARKYLKNNGFGGVLSFFIKGNEKQAATVIDNLSLISHVANVGDTRTLIIHPATTTHE
                     QLSKEAQLASGVYPNMLRLSLGLEHIDDIKYELDEALSKL"
     gene            complement(7736..8281)
                     /locus_tag="PLI30_04540"
     CDS             complement(7736..8281)
                     /locus_tag="PLI30_04540"
                     /inference="COORDINATES: protein motif:HMM:NF026219.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4488 domain-containing protein"
                     /protein_id="HOI78857.1"
                     /translation="MKTKLFYSVFVCVIALLAGCGTAQKGLKSETAKRLFGLWEVKAI
                     HNSNEEGYKKMPHGMFKMIIDDGRFVNFMTTAGGAMITVDGTYRLVGDSLYIEEINHS
                     FNRTQVGKDNPLNLKLSGPKFMYLRWFQAVDEFGENQNQWIEEIWQRVELEDMEVSRI
                     DLEQELRALVKDPEVIKKVMD"
     gene            complement(8396..>8714)
                     /locus_tag="PLI30_04545"
     CDS             complement(8396..>8714)
                     /locus_tag="PLI30_04545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005640134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008239 - dipeptidyl-peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0070009 - serine-type aminopeptidase
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="S46 family peptidase"
                     /protein_id="HOI78858.1"
                     /translation="FVVPEKLKELYNKGDYGRYALPGGKMPVAFMASTHTTGGNSGSP
                     VMNAGGELIGINFDRNWEGVGGDIQYLPDYQRSIIVDIRYVLFIIDKFAGATHLIEEM
                     DIQ"
BASE COUNT         2641 a         2085 c         1801 g         2177 t
ORIGIN      
        1 gagtaatacg ctaaacaatc tcatgattaa tctaggcagt gttaaatcgg cagacatgct
       61 ggacgcttca tacgcttctt acatcaaacc gaagggatat atgggaaaaa ggttccaaaa
      121 ccaattttca acgtatacaa acgatccttc aatcgggttc ttcttcgacg gacttcacaa
      181 caccattgac ccaagagctt acaaactcta cgccataccc ggtgatttca ccagccccga
      241 tgcacaattt acatcggaag atgatttagg ccctttggaa agatctgtgt ttgcagataa
      301 agaccaaaat aatgaaatcg ccactgttaa tttagcgttt acgtggaaca ctatgccagg
      361 tggaagctgg ggagacatgt ctgccatgaa ccagattata ggatctttat acaaccctat
      421 actcgtaaaa aaatacagga atcattcgca aaaacgcgta ttctttgctt cttgggaaac
      481 ctatttccta ctggctgaag cagctctaag aggctggaca acgcccacgt ctgcaaaaga
      541 agcttacgaa aaaggaatta aagccagcct ggactatcat ggcgttagca gtttttacga
      601 cacgtatatt gcttcaacag attacaaccg tgttggaaca tccgtaaaat gggatcatac
      661 agcagagcct cctgcaaccg ttgaagtaga cattattgat ggttatacta accaagcggc
      721 gaagtttgcg tataaatttc cggtagcaag ccagacttct tataagaaag cattgaacga
      781 ccagatgacg aaagtcatca cccaaaaatt catcgctcaa aatccgtggt taccactcga
      841 aacctggaac gattaccgtc gtcttggact cccattcttc gaaaacatgg ttgtggaaaa
      901 cccgttaaca aatcttccgg ctataacgaa agataatgta aaaaccaccc aacaacccga
      961 tttcttccct caacgattaa aatatcctgc atcacttgaa aacagtaacc ctgaaggata
     1021 taaacaagcc gttgaattac tcggaggaac cgacgcagtg ttaactccgc tttggtgggc
     1081 aagacactaa ccggctcaac acttccaatg aaagagaggc tgcccccaaa aaggcagcct
     1141 ctgttcaatg cccagatgga agaagttacc gaacccgggg aatttacaat catgatcgga
     1201 gcggcttcca gcgatatccg gttaagagga agttttctct gcccgtgacg atcccggttg
     1261 ttgtaaggtt aagccgggca gggttttaac gtttatttaa ttcttttttt ctttaatgtc
     1321 gggataatat cgtaaatttg gcctccgaaa aaacattcaa catttaataa tttcaattat
     1381 gagcttttta acaaacgaaa aattaaccat cgttggcgct gccggaatga ttggttcaaa
     1441 catggcgcaa actgcggcaa tgatgcgtct aaccccgaac atctgcttat acgacccctt
     1501 tgctctgggc ctggagggtg tttacgaaga aatgcgccat tgtggatttg aaggagtgga
     1561 ttttacgttc accaccgata tccgggaagc attcaccggc acaaaataca tgatttcttc
     1621 gggcggtgct ccgcgaaaag acggcatgac ccgcgaagat ttgttgaaag gcaatgcgga
     1681 gattgcagct caactgggta aagacatcaa ggcttactgc cccgatctga aacaccttac
     1741 cgttattttt aatcctgccg acattaccgg cctggtggcc cttattcact cgggcctgaa
     1801 accgggctgc gttaccacac tggccggact ggacagtacc cgtttacaaa gcgaactggc
     1861 aaaacatttc ggtgtaaaac agagccaggt taccaatacc cgcacctatg gcggacacgg
     1921 cgagcaaatg gctgtttttg cctctacggc aaaagttgac ggaaaaccgt taaccgacct
     1981 catcggcaca cccgagctca caaacgaaga ctgggatgcc cttaaactaa gagtaaccaa
     2041 agggggcgca aacatcatta aactgcgtgg acgctcatcg ttccaaagcc cgtcgtacgt
     2101 atcggtagaa atgatccgcg cagccatggg cggagagaaa ttcggttggc cttcgggttg
     2161 ttacgtaaat accgtcgatt tcccgaacat catgatggca atggaaacca acatcaccaa
     2221 agacnnnnnn nnnnagacgg tgtggtcata aacgagacca aaggaaccga cagcgaaatg
     2281 acggccttga aagaaagcta ccaacacctg gcaaagctgc gtgatgaagt catcgctatg
     2341 ggaatcatcc ccgcaactga tcaatggaaa tcggtaaatc cgaacttata aatttataaa
     2401 aaactccagg attgatatga accccgaaag ttagacaaaa aactttcggg gtttttttga
     2461 ctaccgtcac agaaggctcc aggcaaccgt cagccccgcc atcacgatag aaatcatact
     2521 catcagcttg atcaaaatat tgagcgaagg cccggatgta tccttgaacg gatcaccaac
     2581 cgtatccccc accacggtgg ctttgtgcac gtcactgcct tttccgccca ggttaccttc
     2641 ttccacgtat tttttggcat tgtcccacgc accgccggag tttgccataa aaacagccaa
     2701 cacaaatcca gaacccaatc ccccggccaa tagtcccatg acaccgggca ctccgagaat
     2761 aacccccgtt acaatgggaa tggcaatggc cagaagtgac ggcaacaaca tctcccgttg
     2821 cgcacccttc gtggatatct caacacactt cgcatagtcg ggagtacccg taccttctaa
     2881 tatgcctgcg atttcacgga actggcgacg tacctcttcc accattttct gagcagctct
     2941 tcccacagca ttcatggtaa gcccacagaa aagaaatgcc atcatcgaac cgataaaaat
     3001 gccgacaagc accttgggat tcatcagggt aacctgataa tagttcatga aatcggcaaa
     3061 accggcttta gcaatttcca cagcatcact gccatcggca aactcaagta cagtttgtcc
     3121 aagccgcaac atacctattt taatctcttc gatataggag gccaacaaag caagtgccgt
     3181 caatgcagcg gaaccgatcg caaatccttt tcccgtggca gcagtagtgt ttccaagagc
     3241 atccagggca tccgtacgtt tgcggacctc ttttcccaat ccgcacatct cagcgtttcc
     3301 gccggcatta tccgcaattg gcccgtaagc atctgtagcc aatgttatcc cgagagtcga
     3361 aagcatcccc acagcggcta ttccaatgcc atacaatccc atactcaggt tctctcccga
     3421 catcatgttc tgcacatcaa acccagtggc acaaaggtaa gaaaacagaa tagccaacgc
     3481 tatggttatc acgggaataa atgtagatat cattccggtc cccatacccg agataataac
     3541 ggtagccggc ccggttttgc cactttctgc aatttgttgc gttgggcgga aagaatgaga
     3601 tgtataatat tccgtacctt gtccgataac aattccagcc agcaatccgg aaattactga
     3661 gaaagataac cctaaccagt tttcgaaacc cagaaggtac aaaataacaa aagtgaacag
     3721 ggcaatcaat accgcactta cgttaactcc gcgattcaat gcggccatca gttttttcat
     3781 atcggcattt tcacctgtat gcaccagaaa gatacccagt atggaaagaa aaacaccgat
     3841 agccgcaata atcatgggcg caaaaaccgc tttggcctgc atagcagggt tcagcacgaa
     3901 agccgaagca cccagtgcag ccgtagccag gatagaaccg cagtaagact cataaaggtc
     3961 ggctcccatc cccgccacat ctcctacgtt atcacccacg ttatccgcaa tggtagcagg
     4021 atttcgcgga tcatcttcgg gaataccggc ttccactttt ccgaccaggt cagctcccac
     4081 atcggccgcc ttggtgtaaa ttcctccccc cacacgtgcg aacagcgcct gtgtggaagc
     4141 acccataccg aaggtaagca tcgtagtggt aatcatgata agcttgtgtc cgggctccgc
     4201 actgtcaatg aaatgatcca ggacaagata ccacgcagaa atatcaagta aggctaaccc
     4261 cacaaccacc aggcccataa cggcgcctga gcggaaggct acctgtaatc ccttgttgag
     4321 tgaattttta gcagcattcg ctgtacgtgc cgatgcataa gtggcggttt tcataccaaa
     4381 aaaacccgct aacgcagaaa aaaaacctcc ggttaaaaat gcaaagggaa cccagttgtt
     4441 ctgcaaccca aaataagcca taacggcaaa aaccgaggct aaaataacaa agacgatagc
     4501 aacaacttta tattgttgct tcaaataaga catggcacct tcgcggacat atttcgcgat
     4561 ggtcttcatt tgttctgtac cttcactttc tctcatcatc ttcttgaaga aatagaaagc
     4621 aaaacttaga gcgaacaacg atgcaatggg gacaagataa aagtaattca ttgataatta
     4681 aaagttaatt tgtattttag gtttacgatt ttaagaaatt ccgataaagt tataaaatat
     4741 gatcgaatta tcgccgataa agttaaatta ttgttatttt tttattattc ctgttttttt
     4801 gtgcaagaac cggcacttaa attttacatt tttattatct ttgccctaat ttattatatg
     4861 gtttaaaaat tcagacaatt tataaatatt gcctttaact aacctttaaa atcaggatac
     4921 aacatatttt ctataaaata aagagtgaga tggaaaagag tttcttctta agcaccgaca
     4981 aaagccccaa aaacatccag ataaaaaaag agataatcaa ccatttcgtt aacaacggaa
     5041 atgacacgat agccgaactt tcaaaagggc tcgacctgag tgttcctact gtaacaaaat
     5101 tgatcgggga gctgagcgat aaaggattgg ttgccgattt cggaaaaata caaaccgcag
     5161 gcggaaggta cccaaatgtt tacggcttga atccgtcatc tgtctatttc atcggggtag
     5221 acatgacaag acaccattta aacattgcgt tgatggattt taaaggcgat attgtgaagt
     5281 acgaaatggg aatttcgtat atgtatgaaa acaccacaga ctcgttcgat gtcctttgtg
     5341 agcacataca aaaattcatc cgtgaaacgg gtgaactgaa gcaaaaaata tacaacatct
     5401 gcctgaatat ctcgggccgc gtaaaccccg aatccggata cagctacagc agcttctatt
     5461 ttagtgaaga gccgatcagc aatatcttat caaaccgatt gaactataaa gtaactgtgg
     5521 acaatgacac gcgctccatg gcttacggtg aatttatgaa gggagcggta cagagtgaaa
     5581 gtaacgtgat ttttgtaaat gtaagctggg gcctcggcat tggaatcatt atcgataaga
     5641 agctatacta cgggaaatcg ggattttcag gagaatttgg ccatttcccc attttcgata
     5701 acgaaatcat ttgccattgc ggcaaaaagg gatgcctcga aacagaagcc tccgggctgg
     5761 ctatacaccg gatggtactt gagcggatca acaagggaga aagttccatt ctcacccaac
     5821 aggtaaaaga cttaaataaa cttaccctta cagacatcgt aaaagccacc aacatggaag
     5881 acccgctttg cattgagata atggaagagg tagggttcaa actgggtaaa tacgtcgccg
     5941 gactgattaa tattttcaat ccggaactgg tggttatagg cggtcaggta gcagaaacag
     6001 aaggatttat tatgcttccg ataagaagtg ccgtcagaaa acattcgttg ggcctggtta
     6061 ataaagatac gcagattatc ccttcgaaat taaaaaacaa agccggagtc gttggcgcat
     6121 gcatgattgc ccgaagccgg ttatttgaca actaacataa cttttctcct gcgaaacatc
     6181 ctgtaatcag ccataaattg cacatttaac acttatttgt gccaagatcc cgtattttct
     6241 tttacttttg cactatagca ttttgtcaca caaaacggat tgttgatttt aaatagtcgt
     6301 caaacgaaca attttgctgc atttaggtta ttttaaaaac ttcaataaca attaaagaaa
     6361 atgagaaatt tacatttcga aaccctgcag attcacgcag gacaacagcc agatcagatt
     6421 gaaggctcaa gagtcgcacc cattcatcag acaacagctt tcgtttttaa gaatgcggag
     6481 cacggagccg acctattcgg cttaagggag tttgggaata tttatacccg gctgcaaaac
     6541 cctaccacag atgttttcga aaaacggatg gccgccctgg aaaaagggac agcagctgta
     6601 gccacagcct caggacagtc tgcagagttt atcaccatca acagcctggc cggtgccggc
     6661 gacaacatag tcagcacatc attcttatac ggtggcacat acaatctttt taaggtttct
     6721 tttgcccggt tgggagttga gttccgtttc gccaaagggg atgatgtaga cagcatagaa
     6781 agcctgatag acgaaaagac aaaagccatc tacctcgaaa tgatcggaaa cccggagtac
     6841 aacatccccg atgtagaaaa aattgttgat ctggcacgca aaaaagacct gccggtgata
     6901 atggataata cgtttgcgca aggtggatac ttgttcaatc ccatcgactg gggagtaaac
     6961 attgtgcttc attccgccac aaagtggatt ggaggacacg gtacatccat gggtggaatc
     7021 attatcgacg gaggaaattt caactggaac aacgggaaat accccactct cagtgagcct
     7081 tctgaaggat atcacggatt aaatttctgg gaggttttcg gtgatcaatc tcctttcggg
     7141 aacatcgctt tcgccatacg ctgccgcgtc gaggggctgc gcgactttgg gcctgccatt
     7201 agcccattca actcctttat gatgcttcag ggcctggaaa cactttcact tcgtgcagaa
     7261 cgaataaatg caagtacatt ggagattgcc cgttggctcg aaaaacatcc gaaagtagaa
     7321 aaagtcaatt atccgggact ggaaagcagc aaatacaacg ccttagccag gaaatattta
     7381 aaaaacaacg gttttggcgg agttttgtcc ttttttatca aaggcaatga aaaacaggcc
     7441 gccacggtca tagacaacct gtccctgatc agccacgtag ctaatgtggg ggatacaagg
     7501 acactgatca ttcatccggc gactaccaca cacgaacagc tttcgaaaga agctcagctt
     7561 gcgtctgggg tttatccgaa tatgttgcgg ttgtcacttg ggctggagca tatcgatgat
     7621 atcaaatatg agctggacga agcgttgtcg aagttataaa aattaaggta tacacagtaa
     7681 aagagcgtgc ggtcagaata tcacacgctc ttttcaattc gttccgcaag agaggttaat
     7741 ccataacttt ctttatcacc tcaggatctt tcaccaacgc ccggagttcc tgctccaaat
     7801 cgatacggct cacttccata tcttcaagct ctacccgttg ccagatttcc tcaatccatt
     7861 gattctggtt ctcaccaaac tcatccactg cctgaaacca cctcaggtac ataaacttag
     7921 gcccggaaag tttcaaattc aagggattgt ctttcccaac ctgtgttctg ttgaaagaat
     7981 gattaatctc ttcgatgtaa agggaatctc caaccaacct gtacgttccg tcaacggtga
     8041 tcatagcgcc acctgcggtt gtcatgaagt taacaaacct gccgtcatcg ataatcatct
     8101 taaacatccc gtgaggcatt tttttgtaac cttcctcatt gctgttgtga atggctttca
     8161 cctcccacaa tccaaacaag cgcttagctg tttcgctttt caatcctttt tgcgctgtcc
     8221 cacatccggc cagtaatgca atcacgcaaa caaatacaga gtaaaataat ttcgttttca
     8281 tgattacttg tatttaatag aatttacccc atacaaatat atgatctttc taggtattaa
     8341 tattattttt tgcagctttt tttcttaaaa aaacaaggct gctgcaacct cttttttact
     8401 ggatatccat ctcctcaatc aagtgtgtag caccagcaaa tttatcaata atgaataaca
     8461 cataacggat atccactata atgcttcgct gataatccgg aaggtactga atatctccgc
     8521 caactccctc ccagttacgg tcaaagttta taccaatcaa ttctcccccg gcgttcataa
     8581 ccgggctgcc cgagtttcct ccggtagtgt gcgttgaggc cataaatgcc accggcattt
     8641 taccgccggg taaagcataa cgtccgtagt cacctttgtt atagagctct ttcagctttt
     8701 caggcacaac gaac
//
LOCUS       DAOSDR010000078         8709 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrimonas sp. isolate AMR_MDS_1826
            NODE_3009_length_8709_cov_4.531199, whole genome shotgun sequence.
ACCESSION   DAOSDR010000078 DAOSDR010000000
VERSION     DAOSDR010000078.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863355
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrimonas sp. (wastewater metagenome)
  ORGANISM  Petrimonas sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Dysgonomonadaceae; Petrimonas.
REFERENCE   1  (bases 1 to 8709)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8709)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:10:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,720
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 35
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8709
                     /organism="Petrimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3009_length_8709_cov_4.531199"
                     /isolate="AMR_MDS_1826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2023866"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1160
                     /gene="radA"
                     /locus_tag="PLI30_04550"
     CDS             <1..1160
                     /gene="radA"
                     /locus_tag="PLI30_04550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019540241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HOI78859.1"
                     /translation="RIDMNDEELNRVLGGGLVPASLVLIGGEPGIGKSTLVLQTMLKL
                     GEIRTLYVSGEESARQLKLRADRIGIENDNCLIVCETNLDEIFKHIKNTSPQLVIVDS
                     IQTVYSDAMESSPGSISQVRECAASILKYAKQTGTPVVLIGHITKEGNIAGPKVLEHI
                     VDTVLQFEGDQHYMYRILRSIKNRFGSTSELGIYEMNQSGLREVNNPSELLLTQNHEG
                     LSGVAISAVIEGIRPFLIETQALVSTAAYGGNPQRSATGFDIRRMNMLLAVLEKRAGF
                     KLAQKDVFLNIAGGLRVNDPGMDLAVISSVLSSSLDMAVDRDTCLTGEVGLSGEIRPV
                     NRIEQRITEAEKLGFSRIIVPANNLKGFKSKVKIEIAQVKKVSDAFRLLFS"
     gene            1227..1297
                     /locus_tag="PLI30_04555"
     tRNA            1227..1297
                     /locus_tag="PLI30_04555"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1259..1261,aa:Cys,seq:gca)
     gene            complement(1357..2511)
                     /locus_tag="PLI30_04560"
     CDS             complement(1357..2511)
                     /locus_tag="PLI30_04560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019538369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HOI78860.1"
                     /translation="MEKDDRKPGNPEQKLVTVAIHSYEKAVILKSILESEGIPAVIHG
                     VSIIQPTVPGNVRVRINESDLPRALTIIEQVDFTSNVESEDDLEKVKVKNEVLIPVDF
                     SNYTLKTCEFGFNLAFDIGCEVKLVHSFFTPYYPMAIPFGDSFSVQTSDKDLYKDIRN
                     KMESEMNTLVKNISERIANNEFPDVKFSYLLMEGLPEEEIISYSKKQRPRAIVMGTRG
                     SNAKDLDLIGSVTAEVIDGCKTPIFAIPENSKDQDISQMKNILFLTNFREREFKAFDI
                     MMGFIKPYPVKVYLAHMARKEDMWDEIKLSGTQKYLEEQYPDLDIDYEVIDRDKQLEV
                     VLEEFVKEHEIDMIAMSSSRRGLFARIFNPGIARRMLFHSDTPLLVIKGL"
     gene            complement(2607..2903)
                     /locus_tag="PLI30_04565"
     CDS             complement(2607..2903)
                     /locus_tag="PLI30_04565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019538368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HOI78861.1"
                     /translation="MARTLTFNELRALKDKLPDGSIHRIAEELGLKPETVRNYFGGYN
                     YKEGKSIGVHIEPGPDGGIVVLDDSTIFDKALSILGLEDYPVYQEEPVEEEDNI"
     gene            complement(3037..3738)
                     /locus_tag="PLI30_04570"
     CDS             complement(3037..3738)
                     /locus_tag="PLI30_04570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019538367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HOI78862.1"
                     /translation="MKEAVENFLPVERDLFFALNGSDSIFLDNLFWTFTGRYVWVPLL
                     LFLVVVFFYKSPRREGILATVFLILLFALCDQVSSGLFKPFFERFRPTHHPDFKELVD
                     TVNNYRGGRFGFISGHATNSFGLAVFFSLVFRNRWVTLPVFTWAILNSYSRIYLGVHF
                     VSDIVAGAIIGSLLAWMLYELYAWIRIRYFHVPAREGRLSVYPAKSGKITGVVFTVYI
                     LLILFFSPFLATLPH"
     gene            complement(3735..4499)
                     /locus_tag="PLI30_04575"
     CDS             complement(3735..4499)
                     /locus_tag="PLI30_04575"
                     /inference="COORDINATES: protein motif:HMM:NF018095.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOI78863.1"
                     /translation="MQTIKNTLTLVLLFFATVSSAQSTVETASEAYRSEDYKRSIQLY
                     EQVVAQQLSEGKESAEIYYNLGNAYFRNGETAKAILNYERALLLNPGDSDIRHNLRFA
                     RTRIEDKIDTSESFFLTTWVNSFKNLFNSNTWATIAIALFIAFLSCIATFLFVRKVWI
                     KKTAFYTGIVIFALLLLSNVFAFSQKNNRIHRNTGIVMAASASIMASPDANSQELFRL
                     HEGTKVKLNKTDGNWIEIEIANGSVGWTSKENVEVI"
     gene            complement(4501..6348)
                     /locus_tag="PLI30_04580"
     CDS             complement(4501..6348)
                     /locus_tag="PLI30_04580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006799769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BatD family protein"
                     /protein_id="HOI78864.1"
                     /translation="MKYFLEKYRVTFLFLVLLLTGTTTLQAQVTFRASAPNGVVKGEQ
                     FRLSYTLNQEGKDLRLPDLKGFDVLFGPSTSRSFSQSTVNGKTTSESSVTYTYILVAP
                     EEGTFTIEPAAITVNGSSYRSNALTVKVLPPDKTAENRQGGNSGSAGSGSSSAAPATT
                     VSANDAFIRAIVSKNSMYEQEGFTVTFRLYTTLNITDLGKIEFPEFEGFMVEEVTIAS
                     NQQLQMERYNGRNYYTADLKKTLLFPQRSGKITIPSGRLEMVFSVPSGKKVSTFFGTQ
                     EVMADVKKVLVTNPLTINVKPLPEGKPLTFSNAVGTFAFAPSISTQQTRANEPVTVSV
                     EISGTGNMKLIQNPEIKFPSNFEIYDPTVTNNFQVTTNGLTGSRKIEYLAIPRYEGDY
                     DIPSIEFSYFDLNTHSYKTLKSPDYNLQIAKGDPGKASASSYVNQQNVRVEQDIRFLK
                     TEGPQYQYQNNFFAGSTGYWLWYLIPLIALIVFYLFNRKMARENADVARMRTRKANKM
                     AIRRLKVAEKFLKEHNKEKFYDEVLRALWGYFSDKLSMPVANLTKDNIEKELSDYGMD
                     NELTGKFMQILNTCEFARYAPAESDAAMDKLYNETVDAIGKMESKLKRK"
     gene            complement(6392..7096)
                     /locus_tag="PLI30_04585"
     CDS             complement(6392..7096)
                     /locus_tag="PLI30_04585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006799771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOI78865.1"
                     /translation="MRLNILKYSLLLMMMALTLPLSAQKEVRKNVRKGNKAYQQQKFS
                     EAFTFYNDALAENPSSPEANYNAGNTLYRQKEWDKSMESYQQYLALEKENPKKMSAAW
                     HNIGNSLLQKKELEKSMEAYKMALRMNPEDEETRYNLAVVQKMIQDQQQDQQQEQQQQ
                     QNQDQQQQEPQNNQDQPKKPEEQQEPEQMSRDNARQLLQAIEQDEKETQEKVNKIKAE
                     ERKQKADENRRRNRDW"
     gene            complement(7093..8115)
                     /locus_tag="PLI30_04590"
     CDS             complement(7093..8115)
                     /locus_tag="PLI30_04590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008669010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HOI78866.1"
                     /translation="MFRFENPEYLYLFIAMPFLVALYIYLNIRKRNDVKKLGVLSTVK
                     KMMPELSLKRSYLKFWLIFAALCTGIIMIARPQFGTKVENVEKEGIELVIAIDVSNSM
                     LARDVSPDRLSRAKQILSRIIDVRKNDKVALIVFAGEAYVQMPLTSDTQSAKLFLNTI
                     DPSLVPVQGTAIAEAITLGVSSFSGDQDVDKAMILITDGEDHEGNANEAARQASDAGI
                     MINVIGIGSVEGTPVPESEYSNNYRTDSGGQVVVSRLNEQMAQEIAQNGKGLYVQADN
                     SNTAVRALETELNELETKKTTSLSYSEYDEKFPFFAWILLIILIVEFLIYDKKNPLFK
                     NIRLFR"
     gene            complement(8147..>8709)
                     /locus_tag="PLI30_04595"
     CDS             complement(8147..>8709)
                     /locus_tag="PLI30_04595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007656551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HOI78867.1"
                     /translation="CPMTTDHKVLLNLLNGIDFGLIEDGTAIGLGLANAVNRLKDSES
                     KSKVVILLTDGSNNRGQIAPLTAADLAQSYGIRVYTVGAGTKGMAPTPVQTPFGMRLQ
                     NMPVDIDEKTLTEIASLTGGKYFRAEDNESLSNIYSEIDELEKYLISVQNVTRKQELF
                     LPFALLALGLILMELVLRRTWLRNIP"
BASE COUNT         2228 a         2087 c         1925 g         2469 t
ORIGIN      
        1 ctagaatcga catgaacgac gaggaattaa accgtgtact gggtggcgga ctggtacctg
       61 catccctggt actgattggg ggagagccgg gaatcggtaa atccaccctc gtactgcaaa
      121 ccatgctgaa gcttggagag atcagaaccc tctacgtttc cggtgaagag agcgcgaggc
      181 aattgaaact gagggccgac cgcatcggta ttgaaaacga caattgcctg attgtttgcg
      241 agaccaatct cgacgaaata ttcaaacata ttaaaaacac ctctccgcaa ctcgtaattg
      301 ttgactccat ccaaaccgtt tacagcgatg ccatggagtc atcgcccgga agtatttccc
      361 aggtgcgtga atgtgccgca tccatcctca agtatgccaa gcaaacggga acacccgtcg
      421 ttcttatcgg acacatcacc aaagagggta acattgccgg ccccaaggta ctggaacata
      481 ttgtggatac tgtgttgcag tttgaaggcg accaacacta catgtaccgc atattacgaa
      541 gtatcaagaa ccggttcggc agcacatcag aactgggtat ctacgagatg aaccaatcgg
      601 ggcttcgcga agtgaataac ccgtccgaac ttttgcttac ccagaaccac gaaggattaa
      661 gcggcgtggc catctcagcg gtaatcgagg gcatccgacc ttttcttatc gagacacagg
      721 ccctggtgag cacggcagct tacggcggga atccgcagcg ctcggcaacg ggattcgata
      781 tccggcggat gaacatgctg ctggcggtac tggagaaacg cgcagggttt aagctggcac
      841 aaaaggatgt tttcctgaat attgccgggg ggttgcgtgt aaacgaccct gggatggatc
      901 tggcggtgat cagctcggtc ctttcgtcga gcctggatat ggccgtcgac agagatacct
      961 gcctcaccgg agaagttgga ctatcggggg aaatccgccc ggtaaaccgt atcgaacagc
     1021 ggattaccga agccgaaaaa ctaggcttct cgcgcattat cgttccggcc aacaacctga
     1081 aaggttttaa gtcaaaagta aaaattgaaa ttgcgcaggt gaagaaagta agcgacgcgt
     1141 ttcggttatt attttcataa attgtaggga agagttttgt tcacaaaaat aaatacatta
     1201 tctttgcatt cgctttatgc aaaatcggta ccttgaccga gtggctaggt agcggtctgc
     1261 aaaaccgtct acggcggttc gactccgcca ggtacctcta aaaagaggcc gtctcaaaaa
     1321 ggaaattatt tctgcgggac ggcctctttt cgttttttat aatcccttga tcaccaataa
     1381 cggtgtatcg gaatggaaaa gcatccttcg cgcgattccc gggttaaaga tacgggcaaa
     1441 cagtcctctt ctcgagctcg acatggcgat catatctatc tcatgttcct tcacaaattc
     1501 ttcgagtacg acctcgagtt gcttgtcacg gtcaatcacc tcataatcaa tatcgagatc
     1561 gggatattgt tcctccaggt acttttgggt tcccgacaac ttgatttcgt cccacatatc
     1621 ctctttcctg gccatatgtg ctaaatacac tttcacagga taaggcttta tgaaacccat
     1681 catgatatcg aaagccttaa attcacgttc cctgaagttg gtcaggaaaa gaatgttctt
     1741 catttgagaa atatcctggt ccttggagtt ctcgggaatg gcgaagattg gcgtcttaca
     1801 gccgtcaatc acttctgcgg taacactgcc gatcaggtca agatcttttg cgttgcttcc
     1861 ccgggtaccc atcacaatag cccgtggacg ttgttttttg gaataggaga tgatctcttc
     1921 ttcgggaagg ccctccatca gcaagtatga aaacttgaca tcgggaaatt cgttgttggc
     1981 aatcctctca cttatatttt tgaccaacgt gttcatttcc gattccatct tgtttctaat
     2041 gtctttataa agatccttat cggatgtctg gactgagaaa gaatctccaa aaggtatggc
     2101 catcggataa taaggggtga agaaagaatg gaccaatttt acttcacagc cgatgtcaaa
     2161 agcgagatta aatccgaatt cacaggtttt caacgtatag ttcgaaaaat ctaccggtat
     2221 caacacttcg tttttaactt tcactttttc gagatcatcc tcagactcaa cgttagaagt
     2281 aaaatcaacc tgctctatga tggtaagcgc cctgggcaga tcactttcgt ttatcctcac
     2341 acgcacgttt ccgggaacag taggttgaat gatactcaca ccgtgaatga ctgcgggaat
     2401 tccttccgac tcgaggatcg acttgaggat gaccgctttc tcgtaggagt gaatcgctac
     2461 ggtcaccagc ttctgttcgg gattgcctgg ttttctatcg tctttttcca tgttcttatc
     2521 gttaaaatgg tttatagcta tcaaaagaaa aaacccaata tttgcctgta atccatcaaa
     2581 tattgggctt catctctcag gatggatcag atattatcct cttcctcaac aggttcttcc
     2641 tgataaacag gataatcttc aagtcccaat atggacaacg ctttatcgaa tatagtagag
     2701 tcatctaaaa caactatacc tccgtcgggg ccgggctcga tatgaacacc gatacttttg
     2761 ccttctttgt aattgtatcc accaaaatag tttcttaccg tttcgggttt taggcccaat
     2821 tcttcagcta tccggtggat actaccgtca gggagcttgt ctttcaatgc tcttaattcg
     2881 ttaaaagtga gagttcttgc catggttgct taatttttat gattgataaa tctcgttagt
     2941 gaattatgcc ctaaacttac acaatatttc agataacgcc aattattttc gtcaaaaaaa
     3001 tgacgaaaat gttactttgt atcctctaaa caaacttcaa tgtggtaaag ttgctaaaaa
     3061 agggctaaaa aatagtatta aaagtatata gactgtaaat accacgcctg ttatcttgcc
     3121 gcttttagcc gggtaaaccg ataatcggcc ttcccgagcc ggaacatgaa agtagcggat
     3181 ccttatccag gcataaagtt cataaagcat ccaggcgagc aacgatccga tgatggctcc
     3241 ggcaacaata tccgaaacaa aatgtacgcc caggtagatt cgcgaatagc tgttgaggat
     3301 cgcccaggtg aagacaggta gggtaaccca ccgatttcta aaaacaagcg aaaagaagac
     3361 agccaagccg aaactgttgg tggcatgccc ggatataaat ccaaatcgtc cgccccggta
     3421 gttgttaacg gtatctacca actctttgaa gtcaggatgg tgcgtgggac gaaatcgttc
     3481 aaaaaagggt ttgaacaacc ccgaagaaac ctggtcgcac aaggcaaaca gcaaaataag
     3541 aaaaacagtc gccaggatac cttcccggcg cggcgatttg tagaaaaaca cgaccacgag
     3601 aaacaacagc aacggcaccc aaacgtatct gccggtgaaa gtccagaaga gattatccag
     3661 aaagatcgaa tcgctaccgt tcaaagcaaa aaaaaggtcc cgttcaaccg gcaaaaagtt
     3721 ttcaaccgct tctttcatat tacctccaca ttctctttcg atgtccagcc cacactaccg
     3781 ttggcaattt ctatttcaat ccagttccca tcggtcttat taagcttcac cttagtccct
     3841 tcgtgcagac ggaaaagttc ctggctgttg gcatcgggcg aagccatgat agatgcagac
     3901 gcagccatca cgataccggt gttgcggtga atgcggttgt ttttctgact gaacgcaaac
     3961 acgttggaca aaagaagcag ggcaaaaata acaataccgg tgtaaaaagc cgttttttta
     4021 atccacactt ttcttacaaa aagaaacgtc gcgatgcaag agagaaaagc gataaagagt
     4081 gctatggcta tggttgccca cgtgttggag ttgaacaggt ttttaaagct gtttacccag
     4141 gttgtcagga aaaagctttc agaagtatcg atcttgtctt cgatacgcgt acgggcaaac
     4201 cggaggttgt ggcgtatgtc gctatctccc gggttaagga gcaaagcacg ttcatagttc
     4261 aggattgctt ttgcggtttc tccgttcctg aaataggcat ttccgaggtt gtagtaaatt
     4321 tctgcggact ctttcccctc cgataattgt tgggccacca cctgttcgta cagctggata
     4381 cttcttttgt aatcttcact ccgatacgct tcggaagctg tttccacagt gctttgtgca
     4441 gaagatacgg tggcaaaaaa aagcagaacc agggtcaatg tatttttgat tgtttgcata
     4501 tcactttctt tttagtttgc tttccatttt accgatggca tcaaccgttt cgttgtacaa
     4561 cttatccata gccgcatcac tctctgccgg tgcgtaacgc gcaaactcgc aagtattaag
     4621 tatctgcata aacttaccgg ttaactcatt gtccattccg tagtcggaca gctctttttc
     4681 tatattatcc ttggttaagt ttgccacggg catagacaac ttatcgctga aatatcccca
     4741 aagggcacgc agtacttcgt cgtaaaattt ttctttgtta tgctccttca ggaatttttc
     4801 cgcaaccttc aaccttcgga tagccatctt gttcgctttt ctggtcctca ttctcgccac
     4861 atcggcattt tccctagcca ttttccggtt aaacaggtaa aaaacaatca gtgcaataag
     4921 cgggatcaag taccacaacc agtatccggt ggatccggca aagaagttgt tctggtattg
     4981 atattgcggt ccttcggttt tcagaaaacg gatatcctgt tccacgcgga cgttctgctg
     5041 attcacgtag ctgcttgccg aggcttttcc ggggtcacct ttggctattt gtaaattata
     5101 atcgggcgat tttagggttt tataggaatg ggtattcaaa tcgaaatagg aaaactcaat
     5161 ggacggaata tcgtaatctc cttcgtaccg ggggatagca aggtattcta ttttccggga
     5221 gccggttaat ccgttggtgg tgacctgaaa attattggtc acggtcggat cgtagatctc
     5281 gaaattagaa ggaaatttaa tctcagggtt ctgaataagt ttcatatttc ctgttcccga
     5341 tatttcgacc gaaacggtaa ccggttcgtt tgcccgggtt tgttgcgtgc tgatagaggg
     5401 agcaaaggca aaggttccca cagcgttgga aaaggtcaac ggttttcctt cgggcaaagg
     5461 cttcacattt attgtcaacg gattagtaac caagactttt ttcacatcag ccatcacctc
     5521 ctgggttcca aagaaggtgg aaactttttt tccggaagga acggagaaca ccatctccaa
     5581 ccgtcccgac ggaatagtaa ttttaccgga ccgttgcggg aacaaaagtg ttttctttaa
     5641 atcagccgta taatagtttc gtccgttgta ccgctccatc tggagttgct ggttcgatgc
     5701 tatcgtcacc tcttcaacca tgaacccttc aaactcggga aactctattt tgcccaaatc
     5761 ggtaatattc agtgtagtat agagccggaa agtgacggta aacccctctt gctcgtacat
     5821 gctgtttttc gagacaatag cacggataaa ggcatcgttg gcacttacgg ttgtggcagg
     5881 cgctgcagat gaagaacctg aaccggcact acctgaattt ccgccttgac gattctcggc
     5941 tgtcttatcg ggaggaagca ctttcacagt cagcgcgtta gagcggtaac tgctcccgtt
     6001 cacggtaatg gcggccggct ctatggtgaa ggtaccttcc tcaggagcca ccaggatata
     6061 cgtgtacgtt accgaacttt cagaggtggt ttttccgttg accgtactct ggctaaaact
     6121 ccgcgatgta gatggcccga aaaggacatc aaaccctttc aaatcaggca aacgaaggtc
     6181 tttcccttcc tggttgagcg tgtaactcaa acgaaactgc tcccccttca ccactccgtt
     6241 gggggccgat gcgcgaaaag tcacctgcgc ctgaagggtt gttgtcccgg tcaagagtaa
     6301 gaccagaaaa aggaatgtaa ctctatattt ttctaaaaaa tatttcatta aaattgtaaa
     6361 tctaaaatcg tcatttgtaa ttttatccgt tttaccagtc cctgtttctt cgcctgtttt
     6421 cgtccgcctt ttgttttcgc tcctccgcct ttattttatt tactttttcc tgcgtttctt
     6481 tttcgtcctg ctcaatagcc tgcaatagct gacgggcatt gtcgcgcgac atttgttcgg
     6541 gctcttgctg ctcttccggc tttttcggtt gatcctggtt gttttgcggc tcctgttgct
     6601 gttggtcctg attttgctgt tgctgctgct cctgttgttg gtcttgctgt tgatcctgta
     6661 tcatcttttg aactaccgcc aggttatacc gtgtttcctc gtcttcggga ttcatccgaa
     6721 gagccatctt gtaagcctcc atcgattttt ccagttcttt cttttgcagc aatgaatttc
     6781 cgatattgtg ccaggcagcc gacatttttt tcggattttc cttttccaac gccaggtatt
     6841 gttgatagct ctccatggat ttgtcccatt ctttttggcg gtacagcgta ttgcctgcat
     6901 tgtaattggc ttcgggcgac gacgggttct ctgccagggc atcgttgtaa aacgtaaacg
     6961 cctccgagaa tttttgctgc tggtacgctt tattaccctt gcgtacattc tttcgcacct
     7021 ctttctgagc cgatagcggc agagtcaaag ccatcatcat caacaacaaa ctgtatttca
     7081 atatgtttaa cctcatctga ataagcgaat atttttaaac agcggatttt tcttgtcgta
     7141 tatcaggaat tcgacaatta agataatcaa taatatccag gcaaagaacg gaaatttctc
     7201 gtcatattcc gaataagaaa ggctggttgt ttttttggtc tccagttcat tcaattccgt
     7261 ctccagagcc cgtacggcgg tattggaatt atcggcctgc acatacagcc cttttccgtt
     7321 ttgagcaatc tcctgagcca tttgttcatt cagccgcgag acaaccacct gtcctccgct
     7381 gtctgtcctg taattgttgc tgtattccga ctcaggcaca ggcgttcctt ccaccgaccc
     7441 tatcccgata acatttatca taattccggc atcggaggct tgcctggcag cttcgttggc
     7501 attcccttcg tgatcttcac catcggtgat aagaatcatt gctttgtcta cgtcctggtc
     7561 tcctgaaaag gaacttactc ccaatgttat ggcttcagca atagccgtcc cttgaacggg
     7621 caccaaactc ggatcaatgg tattgagaaa cagttttgcc gactgcgtat cggaagtcag
     7681 cggcatttgg acatatgcct ctcctgcaaa gacgatgagg gctaccttat cgtttttgcg
     7741 aacatcgatg atcctcgaca agatctgttt tgcccgtgac aacctgtcgg gagaaacatc
     7801 gcgcgccagc atggaattgg aaacgtctat ggcaattacc agctcaatcc cctccttttc
     7861 aacgttttcc accttagtcc caaattgtgg acgggcaatc atgataattc ccgtgcaaag
     7921 cgctgcaaaa atcagccaga acttcaagta ggagcgcttg agtgacagtt caggcatcat
     7981 tttttttaca gtagaaagaa cgccgagttt cttcacatcg tttcgctttc taatgttcag
     8041 gtaaatgtaa agcgccacca aaaaaggcat ggcgataaac aagtacaggt attccggatt
     8101 ttcgaatcta aacatttctt ttttttattc tcttaacaca ccccatctac ggtatattcc
     8161 tcaaccacgt ccttctcaac accaactcca tcagaatcaa tcccagggca agcagtgcaa
     8221 acgggaggaa caattcctgt tttcgcgtaa cattctgcac gctgatgagg tatttttcca
     8281 attcatcaat ctcgctgtat atattgctta gactttcgtt gtcctcggcc cggaaatatt
     8341 ttcctccggt cagggatgca atttcggtca gcgttttttc gtcgatatcc accggcatat
     8401 tctgtaaccg cattccgaaa ggtgtttgca ccggtgttgg cgccattcct ttcgttccgg
     8461 cgccgacggt atatacgcgg atgccgtaag actgagccag gtcggccgcg gtgagcggtg
     8521 caatctgccc gcggttgttt gagccgtccg tcagcaaaat caccactttc gatttggact
     8581 cactgtcttt caaccgattc acggcatttg ccagccccaa cccgatagcc gttccgtctt
     8641 cgataagccc gaaatcgata ccgttcagca ggttcaacag aaccttgtgg tcggtggtca
     8701 tggggcatt
//
LOCUS       DAOSDR010000079         8692 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrimonas sp. isolate AMR_MDS_1826
            NODE_3015_length_8692_cov_4.018293, whole genome shotgun sequence.
ACCESSION   DAOSDR010000079 DAOSDR010000000
VERSION     DAOSDR010000079.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863355
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrimonas sp. (wastewater metagenome)
  ORGANISM  Petrimonas sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Dysgonomonadaceae; Petrimonas.
REFERENCE   1  (bases 1 to 8692)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8692)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:10:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,720
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 35
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8692
                     /organism="Petrimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3015_length_8692_cov_4.018293"
                     /isolate="AMR_MDS_1826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2023866"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..306)
                     /locus_tag="PLI30_04600"
     CDS             complement(<1..306)
                     /locus_tag="PLI30_04600"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HOI78868.1"
                     /translation="MKTALKNETRPMRVNMFFLQKVTDIINQKISDPGLSPGSIAKEL
                     NMSVSQLNRKINAASGYSTNAYIIQLKVNHAKKQLVHFDKNIGEIAKNCGFIDLAYFS
                     "
     gene            complement(837..1040)
                     /locus_tag="PLI30_04605"
     CDS             complement(837..1040)
                     /locus_tag="PLI30_04605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI78869.1"
                     /translation="MKTYFGVIQNGRSFKEVKTRLTGLGIKISKYYPGLKIVKFETEK
                     EVSEAKFDFFITIEEEKEDFFIQ"
     gene            complement(1048..2307)
                     /locus_tag="PLI30_04610"
     CDS             complement(1048..2307)
                     /locus_tag="PLI30_04610"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HOI78870.1"
                     /translation="MVKNRYIVLLDSQNEKSIRNVEKGFSVSVTSSEYLSKDNRSFNI
                     IDNNNAVLYKNLGVVVVDDVDEEQLTRSIADSKSPVIYFEKEREFFPADEFTFIDDLK
                     TNVDQLKNKILELENYIRRKPIPKPAVTDLEWGLKAIGMGETQFSGKGIDVCILDTGF
                     DVSHPDFVDRIIEGKSFIEGEEWDKDPNGHGTHCAGIACGNVRNDTGKRYGIAKDSNL
                     KIGKVLSNNGKGTTSSIIDAIDWAITKKFRIISLSLASPVKLNEKPSVLFETVGIRAL
                     DSNCLIIAAAGNDSKRPALPVPVSAPANSLSIMAVAAIDNQMRVARFSNAGINAATGG
                     NVNVCAPGVDVLSSYPKKSGNSGNYVLLSGTSMATPHVSGLAALYMEQFPDLNTREIW
                     ELLEGKAKPIENLKYRDIGKGLIQVIQ"
     gene            complement(2317..2901)
                     /locus_tag="PLI30_04615"
     CDS             complement(2317..2901)
                     /locus_tag="PLI30_04615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI78871.1"
                     /translation="MKEFIHNKVRVIDDFFAEFNQVQKLFAEKSFDFEHRLNTFLTRL
                     SDYFENRGESSKESEALRIRNMLLTVKRGFDPSKMEKISSGKGELLWGFSYNGIESLD
                     RLLQEVYKKEITKLEEGEEILSNLILNLCQQGVLSDEKLKDLDTIPKIEVYWSYLLSQ
                     NGSISVINKKLLTNLIPEDIYLLIEKVVCKITTQ"
     gene            complement(2902..3600)
                     /locus_tag="PLI30_04620"
     CDS             complement(2902..3600)
                     /locus_tag="PLI30_04620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI78872.1"
                     /translation="MIKLSDYLDYLYSEIIQARKKADEKSVLVAKEYANHEYLKFFKA
                     PRFAFPAIKMDIPLKVSDISAQSKYNFKLDDEKFLNDLNERIEAVNKEEQLNISPVTR
                     EQIQNPEFKQLFKTLEKKDQKLVRNIGDEVKKLDVSPQIKSLNLGVFRPQDVASETEG
                     LEMKRILSEAIANSYSLVSTKLNDIYIDPNTSGTEDKDKLFINLHIEMEEEGLRIKTF
                     TDKNGQTIEEIIFE"
     gene            complement(3605..4480)
                     /locus_tag="PLI30_04625"
     CDS             complement(3605..4480)
                     /locus_tag="PLI30_04625"
                     /inference="COORDINATES: protein motif:HMM:NF023085.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2589 domain-containing protein"
                     /protein_id="HOI78873.1"
                     /translation="MKTLKSVLEQDYSKKTKSELIEMLSGLEDVLSPSVYEQISGIDP
                     SELQGLTKKEIIDIIDNFKTGYDQKWESSVARASSDALKLIFGQMAEDDKIFITSDAA
                     SADFAAELGSIDFATIIGGPLDACVKAQSNASISTVSFINEVGFETDTSGTKKLRMAE
                     FKYKKNVPNPEYDPANPGTTSPTIQQDSEINVPFIALLNVPSFRIESCEVDFNVKLNS
                     TYTKNVSSEFGINAGASGGWGPVKFKVDVSYKRSSSTGIKVEKEYSLGVKVRATNDEM
                     PAGLEKVLGLLGSTN"
     gene            complement(4720..7593)
                     /locus_tag="PLI30_04630"
     CDS             complement(4720..7593)
                     /locus_tag="PLI30_04630"
                     /inference="COORDINATES: protein motif:HMM:NF037660.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOI78874.1"
                     /translation="MNLLVKYIHVSRQQLTQLTVRAVVFLFLTVVSCTRTGNTTIEST
                     AFKDSLEKKVYSRNFPADSVLLLLNSFTASKNYAGIYITSCELGNRYRAQSDFSNAID
                     YHQQSLNAALLLKDTIYIAQAYNNIGTNLRRIGVLPEASDYHYQALQITESFQDVNDK
                     ANQKNRVMALNGIGNIALSINDFTEAENKFREALKGEVTIESNLGQAINYANLGAIYQ
                     MRKQYDSAFYYYDLSMIKNQLIGSKLGIGLCHTHFGEIFEDQNEYQKAEDEYRKAYAV
                     MDGMDDKWHWLVSCLAVSRINLKLDNFDEALRYLEKAKETAEEIRSPEHLSEVYALYE
                     MYYSRIGKYRDALENNKLSVMYQDSILSIQKNNQVTDIRINYEREKNLKFIDQLNRER
                     EMQVRETRIILTASAAALLLLISLSVTLWYAFLQRTKKNKTLRKIDRVKSNFFTNITH
                     EFRTPLTIILGHSRQLQNKKVPLEEEKYYLSAIEKQGEQMLRLVNQLLDITKISSGLD
                     KPVWKNGNIVTFIGILIDRYRLFANDKNIFISFYPQSPLIEMDFVPNYITDIFQNLLS
                     NAIKYTSNHGLVSVLITADEKDVKLKVTDNGVGISEDDFERIFDLFYQVPNSGDKQGS
                     GIGLAFTKQLVEHMNGTIEVKSQLNKGSEFTVTLPLRTNEKVNIEKWEPGSDSPYNTN
                     FSVDWKDESKACQSVFEEEVPHVDGKPSILLVEDNPDVLFYVQSLLKDEYNVIAAVDG
                     VDGMEKALRLIPDIIITDAMMPRKDGLTLCKEVKSSTILNHIPVIVITAKTTSEDMLT
                     GLKCGADVYIRKPFQPEELLIRISNLLEFIKVMKVKYMKAVLEEGSKEPMDANMIFLH
                     NVTNLIFKKISNPNLGPQQIADSLNISPSQLNRKVNAISGFSSSAYILHVKIDYSKKL
                     LAKRDKNIGEVAEACGFLDVAYFSRIFKKITGVTPTAYRNLPQ"
     gene            complement(7710..8153)
                     /gene="rplI"
                     /locus_tag="PLI30_04635"
     CDS             complement(7710..8153)
                     /gene="rplI"
                     /locus_tag="PLI30_04635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009276783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HOI78875.1"
                     /translation="MEVILKEDVVNLGYKDDIVNVKKGYARNYLIPQGKAIIATESSK
                     KVLAENLKQRAHKLEQIKATALALAEKMEGVSLTIGAKTSSTGTIFGSVTNIQIADAL
                     KEKGFDVERKSIVIKDAVKEVGNYVAVAKLHKEVSVEIPFEVVAE"
     gene            complement(8172..8438)
                     /gene="rpsR"
                     /locus_tag="PLI30_04640"
     CDS             complement(8172..8438)
                     /gene="rpsR"
                     /locus_tag="PLI30_04640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018108318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HOI78876.1"
                     /translation="MAKQSEIRYLTPVSVDVKKKKYCRFKKNGIKYIDYKDPEFLKKF
                     LNEQGKILPRRITGTSLKFQRRIAQAVKRARHIALLPYVTDLMK"
     gene            complement(8442..>8692)
                     /locus_tag="PLI30_04645"
     CDS             complement(8442..>8692)
                     /locus_tag="PLI30_04645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019538743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HOI78877.1"
                     /translation="DKKTTGFYTFLEFKADPSVVARLEVNFRRDERVIRFLTIKQDHF
                     AFEYAEKRRNNKGGKKQEARTQDTAKVGKKVELEEKED"
BASE COUNT         2419 a         1863 c         1537 g         2873 t
ORIGIN      
        1 ggaaaaatag gccagatcaa taaaaccgca gttcttggca atttcaccaa tatttttatc
       61 aaagtgaacc agctgttttt tggcgtgatt gactttcaac tgaatgatat aagcgttggt
      121 tgaatatccc gaagccgcat taatttttct attaagctga gacacgctca tgtttaactc
      181 ttttgctatt gaaccgggag acaggccggg gtcagatatt ttttggttga taatatcggt
      241 aaccttttga agaaaaaaca tattcacccg catcggtctt gtttcgtttt tgagagctgt
      301 tttcatgttt ttatcttttt tgttttacta catgaatata ggtattacag acaggtgctg
      361 atgatcagtc agaatgaaca gatccttatg gacttatctt ccaaccaaat ccgaagacac
      421 cgttaagcta tatgaagaat aataaaaggg cttgaaaagt atccaacaac agaatatcgc
      481 gcatgttaaa cattataatt ttttaaagag atacatcgag ggccctttta ttggcaatgc
      541 atccattctc atacaaaaat aattcgaggt agaaaaaaac gttaggatta tttacacaaa
      601 atttaggact cagtgagcat taacatttcg catatttgta ttccacacac attatctctc
      661 gaataactaa cttataaaca actaaatacg atctaaaaat atgaataaag tttggattgg
      721 ataaagataa atctgcgaag ggagattatt tcaattgcct catataccac tgcatttcaa
      781 gcaattaaaa aagccccaat gaatatcgga gctctatgtg cggagaaaac acaaaattat
      841 tgaataaaga aatcttcttt ctcttcctct attgtaataa aaaaatcaaa tttggcttcg
      901 gaaacttctt tttcagtttc aaacttaaca atctttaatc ctggataata tttgcttatt
      961 tttataccca agccggttaa tctcgtttta acttccttaa aagacctacc gttctgtata
     1021 actccaaagt atgttttcat attcttatca ttggataacc tgaatcaacc ctttcccgat
     1081 atccctatat ttgagatttt caatcggttt tgctttgcct tcaagtaact cccaaatttc
     1141 ccgggtgttc agatccggaa attgttccat ataaagcgcc gccagtccag aaacatgggg
     1201 agtggccata cttgttccac tcaacaagac ataatttcct gaatttccac ttttcttcgg
     1261 ataagaactc agcacatcca ctcctggcgc acaaacattg acattgcctc cggtagccgc
     1321 attgatcccg gcgttagaga accgggcaac ccgcatctga ttatcaatgg ctgcaacagc
     1381 catgatggag agagagtttg ccggtgcaga aaccggaacg ggcaatgcag gccttttact
     1441 atcattgcct gcagctgcaa taatcaggca attgctatcc agagccctga ttcccacagt
     1501 ctcaaaaagg acagaaggct tttcgttcag cttcaccggt gaagccaggg acaaggagat
     1561 tattcggaat tttttagtaa tagcccagtc aatcgcatcg ataatactgc tggttgtccc
     1621 ctttccattg ttggaaagta cttttcctat tttcaggttg ctgtcttttg caatcccgta
     1681 ccgcttgccg gtatcgtttc taacattccc gcaagcaata ccggcacaat gtgttccgtg
     1741 tccgttagga tctttatccc actcttcacc ttcgatgaag gatttccctt caataatcct
     1801 gtctacaaaa tcgggatgcg aaacatcgaa tccggtatcg agtatgcaaa catctattcc
     1861 tttgcccgaa aattgggttt ctcccattcc aatagctttc aacccccact ccaagtccgt
     1921 tacagccggt tttggaatcg gttttctccg gatgtagttt tccagttcca gaattttgtt
     1981 cttcaattga tcaacgtttg tcttcaaatc gtcaataaag gtaaattcat cggcaggaaa
     2041 aaactccctt tccttttcaa aatagataac cgggctttta ctgtcggcta tcgaccgggt
     2101 tagctgttct tcatctacat cgtcaaccac taccactccc aggtttttgt aaagaacggc
     2161 attgttattg tcgataatgt taaacgagcg gttatctttc gataaatact ccgatgaggt
     2221 aacgcttacg gagaaccctt tctccacgtt acggatcgat ttttcgtttt gactgtccag
     2281 caaaacaatg tacctgtttt ttaccataat tttatttcat tgagtagtga ttttgcaaac
     2341 aactttttcg ataagcaagt aaatatcttc cggtattaaa ttcgtgagca gttttttgtt
     2401 tattacggaa atagaaccat tttgagaaag cagatagctc cagtaaacct cgatcttggg
     2461 aatagtatcc aggtctttca gcttttcgtc cgataagact ccttgttgac aaaggttaag
     2521 aatcaagttg gagagaattt cttcaccctc ttcgagtttc gtaatctctt ttttgtaaac
     2581 ctcctgcaac aacctgtcaa gactctcgat tccgttgtaa gaaaaccccc acaacaattc
     2641 tcctttcccg gaacttatct tctccatttt ggagggatca aaacctcttt ttaccgttag
     2701 gagcatgttc cttatcctaa gagcttccga ttctttagag ctttctcctc tgttttcaaa
     2761 ataatcggat aatctagtca ggaaagtatt taaccgatgc tcaaaatcga atgatttttc
     2821 ggcaaataac ttttggacct gattaaactc cgcaaaaaaa tcgtcgataa ccctgacttt
     2881 gttgtggata aattctttca tctattcaaa aatgatttct tcgattgttt ggccattttt
     2941 atccgtaaac gttttaattc gcagcccctc ttcttccatc tcaatatgaa gattaataaa
     3001 caacttgtcc ttatcttccg tcccggaggt attcgggtcg atgtagatat cgttcaactt
     3061 ggtagatacc aacgaataac tattggctat agcttctgac aatattcttt tcatttccag
     3121 accctccgtc tcggaggcga catcctgagg cctgaacaca ccaaggttca acgatttgat
     3181 ttgaggggaa acatctagtt tttttacttc gtcgccgata ttcctaacca gtttttggtc
     3241 ctttttctca agtgttttaa agagttgttt aaattcggga ttttgtatct gctctcttgt
     3301 aacaggagaa atgttcagtt gctcttcttt atttacagcc tcaattcgtt catttaaatc
     3361 attcaggaac ttctcgtcat cgagcttgaa gttgtatttc gactgagcac ttatatcact
     3421 aacctttaat ggtatgtcca tttttatagc tggaaaagca aatctcggtg ccttaaaaaa
     3481 cttcaggtat tcgtggttgg cgtactcttt ggccacaaga actgactttt catctgcttt
     3541 tttacgagcc tggattatct cgctgtagag ataatccagg tagtctgaaa gtttaatcat
     3601 gatcttagtt tgttgaaccc aataaaccca gtactttttc cagaccggcc ggcatttcat
     3661 cgttggttgc cctgaccttg acgcctaaag aatattcttt ttcaacctta atacctgtac
     3721 ttgacgatct tttgtaggaa acatccactt tgaattttac cggcccccaa ccgccagaag
     3781 ctccggcatt gataccaaat tcactgctta cgtttttcgt gtaggtagag ttaagcttaa
     3841 cgttgaagtc cacctcgcac gattcaatcc ggaaactcgg tacattgagt aaggcgataa
     3901 acggtacatt tatttctgaa tcttgttgaa ttgtcggaga ggtagtaccc ggatttgcag
     3961 gatcatattc aggattagga acatttttct tgtacttgaa ctccgccatc ctaagttttt
     4021 ttgtaccgga tgtgtctgtc tcaaatccca cctcgttaat gaagctcacg gtgcttatcg
     4081 atgcgttcga ttgcgctttt acgcaagcat ccaacggtcc accaataatt gttgcaaaat
     4141 caatactccc tagctctgcg gcaaaatctg cactggcagc atctgaggta ataaaaatct
     4201 tatcgtcttc agccatttgt ccgaagatta attttaacgc atcggatgaa gctctggcga
     4261 cagagctctc ccatttctgg tcgtatccgg ttttaaaatt gtcgataatg tctataattt
     4321 ctttttttgt aagaccctgt aattcagaag gatcaatccc gctaatctgt tcgtataccg
     4381 aaggggatag tacatcttcc agccccgaaa gcatctcaat cagttcactt tttgtttttt
     4441 tactgtagtc ttgttccaag accgatttta aagttttcat cgtgcattaa ttttaataat
     4501 aaaaacaata gaatttattt gcgataaaga ttcaagccgt acaatctgct gacttatcgt
     4561 tgataaaatt aagggtggat ttgaaaaaaa attaggacaa ttctgacaaa aatttggaca
     4621 ttttgaacat taacttcttt tgatttgtac tacaacgacg tagcaaaaaa cataataagc
     4681 ttaatgtcaa catgttaaca tcattaaaaa aaacaaaagt tattgaggca gatttcgata
     4741 ggctgttgga gtaactcctg taattttctt gaatattcgg gaaaaataag ccacatcaag
     4801 aaagccacat gcttcagcca cttccccaat gtttttgtca cgcttggcca gtaacttttt
     4861 ggagtaatca attttcacat gaagaatata ggcagaagaa gaaaatcccg aaatagcgtt
     4921 cacttttcgg ttaagctgcg aaggactaat attcaatgag tcagctattt gttgtggacc
     4981 cagattggga ttggatattt ttttgaaaat taaattagta acattatgaa gaaaaatcat
     5041 attggcatcc ataggttctt ttgaaccttc ctccaatact gccttcatat actttacttt
     5101 catgacctta ataaattcca gcaggtttga aattctgatc aagagctcct cgggctgaaa
     5161 aggttttctg atatacacat ctgcaccgca cttaagtccg gtcagcatat cttcggaagt
     5221 agtctttgcc gtgatcacaa taaccggaat atgatttaaa attgttgaag atttcacttc
     5281 tttacataag gtcaatccat ctttacgagg catcatcgca tcagttatga tgatatcggg
     5341 tatcagcctg agggcttttt ccataccgtc tacaccgtca acggctgcaa tgacgttata
     5401 ctcgtctttt aaaagtgatt gaacataaaa gaggacatcc gggttgtctt ctaccagtaa
     5461 aatggagggt ttgccatcca cgtgaggcac ctcttcctca aatactgatt ggcatgcttt
     5521 tgattcatct ttccaatcta cagaaaaatt cgtgttataa ggtgaatcac tgcctggttc
     5581 ccatttctcg atattcactt tctcgtttgt tcttaacgga agtgtaacgg taaactccga
     5641 gcctttgtta agctggctct tcacctctat ggttccattc atatgctcta ccagctgttt
     5701 ggtaaaagcc agtccaatcc cactgccctg cttatctccc gaattaggca cctgataaaa
     5761 aagatcgaat attctttcga aatcatcttc gcttataccc acaccattat cagtcacttt
     5821 taatttcaca tccttctcat cggccgtaat taaaacacta accaatccgt gatttgaggt
     5881 atatttaata gcattggaaa gtaaattctg aaaaatgtcg gttatatagt taggcacaaa
     5941 atccatctca ataagtggac tttggggata gaaagagata aatatgttct tgtcgttcgc
     6001 aaataaacga taccggtcaa tcaatatccc tataaaagtt acaatgttac cgtttttcca
     6061 taccggttta tccagtccgg aacttatttt agttatatcg agcagctgat ttaccagacg
     6121 cagcatctgt tcaccttgtt tctcgatagc agaaaggtaa tacttttctt cttcaagcgg
     6181 gacttttttg ttctgcagct gcctgctgtg tccaagtatt attgttaaag gtgtccggaa
     6241 ttcatgggtg atattggtga agaaattcga tttaacccta tctatttttc gcaacgtttt
     6301 atttttcttg gtacgttgta aaaaagcgta ccacaacgtt acagacaagg atatcaataa
     6361 caataatgct gcagcagatg ccgtaagtat aattctggtc tcacgcactt gcatctccct
     6421 ttccctgttc aactgatcaa taaacttcaa atttttttcg cgttcataat tgatgcgtat
     6481 atccgttacc tgattattct tctgtatgct aaggatactg tcctggtaca ttacgcttaa
     6541 cttattgttt tctagcgcat ctctgtattt tccgatccga ctatagtaca tctcgtacaa
     6601 agcatagact tccgacaaat gttcgggaga tctgatctcc tctgcagtct ctttagcttt
     6661 ctcgagatac ctgagtgctt catcaaaatt gtccaactta agattgatcc tggaaacggc
     6721 caggcaagag acaagccaat gccacttatc gtccattccg tccattacag cataagcttt
     6781 ccgatactca tcttctgctt tttgatattc attttgatct tcaaaaattt cgccaaaatg
     6841 cgtatgacaa agcccgatcc ccaattttga tccaatcaat tggtttttaa tcatagaaag
     6901 atcgtaataa tagaacgccg aatcatactg cttcctcatt tggtaaatag ctcctaaatt
     6961 ggcatagttt atggcctgac cgagattact ttcgatggtg acttctccct tgagtgcttc
     7021 cctgaattta ttttcggctt cggtaaaatc gtttatggaa agcgcgatgt taccaatacc
     7081 attcagggcc ataaccctgt ttttctggtt cgccttatcg ttgacatctt gaaagctctc
     7141 tgtaatctga agggcctgat agtggtagtc ggacgcttcg ggaaggacgc cgatccttct
     7201 caggttggtt ccaatattgt tgtaagcttg tgcaatataa attgtatcct tcaagaggag
     7261 ggcagcattt aacgattgct gatgatagtc gatcgcattg gaaaagtcag attgtgcccg
     7321 atacctgtta ccaagctcac aactagtgat ataaataccg gcgtagtttt tggatgccgt
     7381 aaaactgttc agcaggagta atactgagtc ggcaggaaag ttgcgtgaat atactttttt
     7441 ctccagactg tctttaaatg cagtgctctc aattgtggta tttcccgttc ttgtacacga
     7501 aacaactgtc aaaaataaaa aaaccaccgc cctaacagtt agctgagtta actgctggcg
     7561 agaaacatga atgtatttta ccaataaatt cattattttt tgtgtctctt ggcggtaaac
     7621 aacagatatc cgcatcgttg caaatgtaat aaaaaaaagc tgataaacac aaattttatc
     7681 agcttttaaa aaattttatt ctgtatatgt tattctgcta ctacctcaaa tggaatttca
     7741 acagacactt ctttgtgcaa cttggcaacg gcaacgtagt ttcctacttc ttttaccgca
     7801 tcttttatca caatgctttt tctctccaca tcaaaaccct tttctttcaa tgcatcggca
     7861 atctgaatgt tggtaaccga cccaaagatc gttccggtgg aactggtttt tgcaccgatg
     7921 gtaagggata ctccttccat tttttcggcc agggccaagg cggttgcttt aatttgttcc
     7981 agcttatgag ctcgttgttt caggttttca gccaacactt ttttcgatga ttcggtagca
     8041 ataatagctt ttccctgtgg aataagatag ttgcgggcat atcctttctt tacgtttact
     8101 atgtcgtctt tgtagcctaa attcactaca tcttccttta aaattacttc cataactttt
     8161 cctccttctt tttatttcat taaatcggtt acgtacggaa gcaaagctat gtgacgtgct
     8221 ctcttaactg cttgcgcgat acgacgttga aacttaagcg atgtacctgt tattctgcgt
     8281 ggaagaattt tcccttgctc attcaggaac tttttcaaaa attcgggatc cttgtaatcg
     8341 atatacttga ttccattctt tttgaaacga caatactttt tcttcttaac atccaccgat
     8401 acgggagtca aatatctgat ttctgattgt ttagccatct tttaatcctc cttttcctct
     8461 aattcaactt tttttcccac cttagcggta tcctgagtac gtgcttcctg cttcttaccg
     8521 cctttgttgt ttcgtctttt ctcagcatac tcaaaagcaa aatgatcctg cttgatagtt
     8581 aagaaacgaa ttacacgctc atcgcgacgg aagtttactt ctaatctggc aaccactgac
     8641 gggtcagctt tgaattccag aaacgtataa aagccggttg tttttttatc ga
//
LOCUS       DAOSDR010000080         8616 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrimonas sp. isolate AMR_MDS_1826
            NODE_3060_length_8616_cov_4.040883, whole genome shotgun sequence.
ACCESSION   DAOSDR010000080 DAOSDR010000000
VERSION     DAOSDR010000080.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863355
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrimonas sp. (wastewater metagenome)
  ORGANISM  Petrimonas sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Dysgonomonadaceae; Petrimonas.
REFERENCE   1  (bases 1 to 8616)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8616)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:10:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,720
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 35
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8616
                     /organism="Petrimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3060_length_8616_cov_4.040883"
                     /isolate="AMR_MDS_1826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2023866"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1249)
                     /locus_tag="PLI30_04650"
     CDS             complement(<1..1249)
                     /locus_tag="PLI30_04650"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOI78878.1"
                     /translation="MKRFLHVLINIAVANTSTFFLWSALSFWLYLETHSVLILSILSG
                     TFMTLVTLSGMFFGTIVDKHRKKEVIAVSSIVALIFLLLAGSVFLFFDRKEIADISSS
                     AFWIFSLSILLSALASNLRSIALSTTVSILVPKEDYARANGMVGMVQGVGMIANTVFS
                     GLVIGRLGLEWAMYITIILSGLALLHLLFVPIPEDHLHHDPEVLNKQVDFKGAIKAIR
                     SVPGLMALIFFTTFNNFIGGVIMVLLDPYGLEMFSVEMWGIVLGITGTGFIFGGMAIT
                     KKGLGKQPLRTLLYGYVIGSLICLLFTVREWAWLFLAGIFLYMFFVPFIEAAEQTIIQ
                     TVVPKIKQGRVFGFAQTVESAATPVSSYLIGPIAQFSIIPFMESDAGKQTFGWLLGEG
                     NARGIALTFVLAGLFMLLLSLLAF"
     gene            1355..1963
                     /locus_tag="PLI30_04655"
     CDS             1355..1963
                     /locus_tag="PLI30_04655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016291314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HOI78879.1"
                     /translation="MVFEHLPEDNTGRLAPGITRREAFALVEKYNKEPFHIQHAETVE
                     AVMRYFATNLGYDGEVDFWGLVGLLHDLDFEQFPDEHCIKVREIFDDEGIDPQLKRAV
                     VSHAYGFTQHDVKPVHQMEKVLYAADELTGLIGAAVLMRPSKSTLDLTVQSLKKKFKD
                     KKFAAGCSREVIRKGADLLGWELDYLMEETIKALQGKERAEL"
     gene            complement(1994..2329)
                     /locus_tag="PLI30_04660"
     CDS             complement(1994..2329)
                     /locus_tag="PLI30_04660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006841767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein YjdM"
                     /protein_id="HOI78880.1"
                     /translation="MTQTPHCTICQSAVTYFDGTLFHCTECFHAWTQEDMTAAENSKK
                     VLDNNGNELFDGDDVTVIKDLKVKGSSMVVKRGTRARGIRLSADDPTHVQAKVDGQTI
                     FILTDFLKK"
     gene            2513..3685
                     /gene="pncB"
                     /locus_tag="PLI30_04665"
     CDS             2513..3685
                     /gene="pncB"
                     /locus_tag="PLI30_04665"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019539120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004516 - nicotinate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0019358 - nicotinate nucleotide salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HOI78881.1"
                     /translation="MAIIKSILDTDLYKFTTSYAYSKLFPRAFGEFVFVDRSNGDYPD
                     GFDERVRQELDKMSELSLTIGEEEFVRKKMPYLPPIYIDFLKGFRFDPSEVKVSLEGR
                     KLKIRATGLLYRITLWETPILATVSELYFRETKQYPDMQYMEKAAIEKALSLKENGIT
                     FSLFGMRRRFSFEVEDRVTGLLKQYAGDSLFGTSNVYLAYRHNVNVSGTHPHEWVQFH
                     GSIYGYKMANYMSMENWINVYDGDLGTVLTDTYTTDVFLRNFSKKHAALFTSLRHDSG
                     DAFVFTDKVIRRYEELRIDPKLKYIVFSDSLNVDKAILIKNYCGERIGATFGIGTNLT
                     NDVGNNIKGMNIVMKLFRCKMTAKEQWHECVKLSDVEGKHTGSEKEIRLAQETLGL"
     gene            complement(3727..5427)
                     /locus_tag="PLI30_04670"
     CDS             complement(3727..5427)
                     /locus_tag="PLI30_04670"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019539121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HOI78882.1"
                     /translation="MKFNILFSCVFLFFSMARFSAYTQHPYTDPLDIPPALSANFGEL
                     RSNHFHSGIDFKTQQTVNKPVVAIEDGYISRISVSPGGYGLALYIDHPSGHTSVYAHL
                     NSFSSPIADFVKEKQYEQESYRVELYPEPGLLIVKKGQQIGLSGNTGSSGGPHLHFEV
                     RDTQTQDPLDPLSFFSRAILDTQKPEIRGIAFYTIEGKGVVNGSSNPLRMNILKNKSG
                     IPLAPPQAINAWGRIGVAVKAYDRMNGQSNIYGVKHVRLFVDEKQVFSSSIDRFPFSK
                     TRMLNSFIDFEEWRERSSFYMKSFIEPGNTLKFYDAVNNGFIDINEERDYRMRYELED
                     YNGNTLVYSFVVVGQQQPVAKTDSCKNFMPWTLHNTFVDFDFMLDIPSGNLYNSFCFS
                     HRKTGSTLYYSDIHRVNDSPVPLHQKATVWIKLNVDTLDNKQQYGIVEITETGNDNWI
                     GGTYKRNGMEVSIRELGRMYAVDSDTFPPNIVPVNPEKWVASRRIQIRLSDNKSGISA
                     FKGTINGKFVLFSHDMKSSLYTYLFDDSRLEKGKTQELVFVATDGAGNTTEYRYTFEY
                     "
     gene            5688..6077
                     /locus_tag="PLI30_04675"
     CDS             5688..6077
                     /locus_tag="PLI30_04675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009860753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HOI78883.1"
                     /translation="MNIENTQSQMRKGVLEYCILAIIRRNEAYPGDIIDEMKNAGLQL
                     LEGTLYPLLNRLKNAEILTYRWVESTSGPPRKYFILTEKGEKFYDLLESTWIELSTGV
                     NVVVNGNYSKEDTVVNDNLNEPNTTEQ"
     gene            6074..8059
                     /locus_tag="PLI30_04680"
     CDS             6074..8059
                     /locus_tag="PLI30_04680"
                     /inference="COORDINATES: protein motif:HMM:NF015957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HOI78884.1"
                     /translation="MKKVININFQGQVIAIEETAYELLKQYIDSLKKYFSREEGGEEI
                     VNDIENRIAELFGNRLKLGINCITDEDVQSIISSIGRPEDFDTEYQESVYAYGETPFE
                     QFASTSEQPKSEPDGSERRQTLYRNANDKIIAGVCSGLAHYFKIDPVWMRIVFVVLFS
                     VLFWVYLILWIVLKPKVLETNVARRLYRNPNDRFLGGVCGGIAAYFKIDTWIPRLLFA
                     APLFLNLMGMISIPFFPWNRFFDNVDFNWNINMSVVVIYAVLWVIIPRATTVKQKLEM
                     MGEEEYIKSIRETVSDNVASVKSKTDDGDNYTKPYAATAAVAGDSEKVSLDAMPPEPP
                     RFHAAKASANATAGSGNTGCLNALGILFKIIFFAFAGIFAVVTTGMMIAFLVAGTKFV
                     PLKSLFIDLGYENTLLWLSAGLLFAVPIISIIVWIVRRSMKAKSRPVIGVVSIILWVV
                     GIFSATMLGFKVAEKFSVESSAENVQALSAFSGDKLYVDMAVYPSDYYSFSRNFGPGS
                     DLDNFPYYTINEDSLLFSNIKLQIVQSQDSLYYVRTISSSSERDLKIARKNAGEFTYP
                     LQQQDSLLFLPEFFSAPISQGFRLQGMIVEIAVPLGKKIEIDERLDDYDNFTSNSSVR
                     RKLKKSRNNKTFDWDYGEEYILENGGLNKTVILTDSI"
     gene            8187..>8616
                     /locus_tag="PLI30_04685"
     CDS             8187..>8616
                     /locus_tag="PLI30_04685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006842907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF389 domain-containing protein"
                     /protein_id="HOI78885.1"
                     /translation="MEKQVRKIVTIFNLRSELEDYETIHAEIEKGIQFRGTNLWILMF
                     AIVVASVGLNMNSTAVIIGAMLISPLMGPINGMGYSLATYDFYLFRRSLKNFIYAVGV
                     SLLTSALYFFLSPINEAHSELLARTSPTIYDVLIALFGGLA"
BASE COUNT         2396 a         1788 c         2056 g         2376 t
ORIGIN      
        1 ggaaagctag cagtgaaaga agaagcataa agagacccgc taacacaaac gtaagcgcta
       61 taccccgggc gtttccctct cccagcagcc agccgaaggt ttgttttccg gcatcgcttt
      121 ccataaaagg aataatggag aattgcgcaa tcggtccgat caggtagctt gaaactggag
      181 tggctgctga ctccacggtc tgtgcaaagc caaaaacccg gccttgtttg atcttcggta
      241 caacggtttg aatgatggtt tgttctgcag cctcgatgaa cggaacgaaa aacatataca
      301 aaaaaattcc cgccagaaac agccatgccc attcgcgaac ggtaaaaagt aaacagataa
      361 ggcttccaat aacatacccg tagagcaacg tccgtaacgg ttgtttgccc aacccttttt
      421 tcgtgatggc catcccccca aaaataaagc ctgttccggt gatgccgaga acaatgcccc
      481 acatctctac cgagaacatt tccaacccgt atggatcgag aagaaccatg atcactccgc
      541 caataaagtt gttgaacgta gtgaaaaaga taagggccat caacccggga acggaacgta
      601 tggcctttat ggcgccttta aaatccactt gtttattgag gacttcggga tcgtgatgca
      661 ggtggtcttc cggtatcggg acaaaaagta ggtgcaacag agccagtccc gataaaataa
      721 tcgtgatata catggcccat tcaagcccca gccggccgat caccagtccg ctgaaaaccg
      781 tgttggctat cattccaaca ccctgaacca ttcccaccat cccattggcc cgggcataat
      841 cctctttcgg taccaggatg gatacggtgg ttgaaagcgc tatcgaacga aggttgctgg
      901 caagcgcact caataagatg gaaagggaga aaatccagaa ggcggatgac gatatatcgg
      961 caatttcttt ccggtcgaaa aacaagaaga cagaaccggc aagtagcaga aaaataaggg
     1021 ctacaataga tgaaacagca atgacctcct ttttccggtg tttgtccaca atcgtgccga
     1081 aaaacattcc cgaaagggtg acgagtgtca tgaaagtccc gctgaggata gagagaataa
     1141 gtaccgaatg cgtttccagg taaagccaga aagacaatgc actccacaga aagaaagtgg
     1201 aggtgttggc tacggctatg ttgatgagca catggagaaa tcgtttcatt cgtgtatccc
     1261 ggagaacaga cttttaaaca acacaactga ttttttgttc ggacggtaat tttgcagaaa
     1321 atggttacct ttacgcgaaa aaaatgtaaa acgtatggtg tttgaacatt tacctgagga
     1381 caataccggt cgtttagccc ctggaatcac ccgccgggaa gcttttgcgc tggtggaaaa
     1441 atacaacaaa gagccgtttc atattcagca cgcggagacg gttgaagccg tgatgcgtta
     1501 cttcgccact aatctggggt atgacggtga ggtggatttt tgggggttgg tgggtttgtt
     1561 gcacgacttg gatttcgagc agtttccgga tgagcattgc atcaaagtcc gcgaaatatt
     1621 cgatgacgag ggcattgatc ctcagctaaa gcgtgctgtg gtttcccatg cttacgggtt
     1681 tacgcaacac gatgtaaagc ctgtacatca gatggagaaa gtgctttacg ccgccgacga
     1741 attaaccggg ttaatcggcg ctgccgtttt gatgcgcccc agcaagagta cgctcgattt
     1801 gacggtacag tcgttaaaaa agaagttcaa ggataaaaag ttcgctgcag ggtgttcgcg
     1861 tgaggtcatc cgcaaaggag ccgatctcct cggctgggag cttgattacc tgatggagga
     1921 aaccattaaa gcgctccagg gaaaagagag ggcggagctg taaaagccca ctgtgccctc
     1981 cccgcttgtt tgtttatttc tttaaaaaat cggtgaggat aaaaatagtt tgtccgtcaa
     2041 ctttggcctg cacgtgcgtg ggatcgtctg ctgaaagacg gattccgcgt gcccgggttc
     2101 cccgttttac taccatggac gaacctttca cctttaagtc ttttattacg gtaacatcgt
     2161 caccgtcaaa aagttcgttt ccgttgttat ccagtacctt tttcgaattt tcggctgctg
     2221 tcatgtcttc ttgcgtccat gcgtgaaaac attccgtaca atggaataac gtgccgtcga
     2281 aataagtaac cgcactttgg catattgtac aatggggagt ttgagtcatg ttgttctaaa
     2341 ttgtttttcc caaagatagg gcttaactgc caaaattcaa aattaccata atttcttgtc
     2401 cccggaaggg aacaaacgag atacatatag tttggaagaa atgctttttt tacaggtgtg
     2461 aaccgattac tcttttaagt gttttagaac taaaactaat tatctgcgaa aaatggcaat
     2521 tatcaaaagt attcttgata cggatttgta taagtttacc accagctatg catacagcaa
     2581 gctttttccc cgtgcctttg gagagtttgt atttgtggac agaagtaatg gcgattatcc
     2641 cgacggattt gatgaacggg tacggcaaga attggacaag atgtcggaac tgagcctgac
     2701 gataggggag gaggagttcg tgagaaaaaa gatgccgtat ctaccaccga tttatatcga
     2761 ctttttgaag gggttccgtt ttgatccgtc ggaagtgaag gtttctctcg aaggccgtaa
     2821 attaaaaatc cgtgccacgg ggttgcttta tcgcattacg ctttgggaaa cgcctatcct
     2881 cgctacggta tctgaacttt atttccggga aacgaagcag tatcccgaca tgcaatacat
     2941 ggaaaaggcg gccatagaga aagcacttag cctgaaagaa aacggtatca cgttttcact
     3001 tttcggcatg cgacgccggt ttagtttcga agtggaggac cgggttaccg gattgttgaa
     3061 gcaatacgca ggagattccc tgttcggtac cagcaatgta tacctggcat atagacacaa
     3121 cgtgaatgtg tcgggaacac atccgcatga atgggtacag tttcacggat caatttacgg
     3181 atataaaatg gccaattaca tgtcgatgga gaactggatt aacgtgtacg acggtgatct
     3241 gggaaccgta ctcaccgata cctataccac cgatgttttt cttcggaact tcagcaaaaa
     3301 gcatgcggcg ttgtttacca gcctccggca tgacagtggc gatgcttttg tttttaccga
     3361 taaagtcatt cgacggtatg aagaactgcg gatagacccc aagctgaaat acattgtttt
     3421 ttcggacagt ttgaacgtag acaaggcgat ccttatcaaa aactattgtg gagaacgtat
     3481 aggggctact tttggcatag gaacgaacct gaccaatgat gtgggcaaca acatcaaggg
     3541 aatgaatatt gtgatgaaac tgttccgatg taaaatgact gccaaagaac aatggcacga
     3601 atgtgtgaaa ctgtcggatg tggaagggaa gcataccgga agcgaaaaag aaatccgcct
     3661 ggcacaggaa acgctgggac tctgaaacgc gctttgggcc aggtgataag ctctcaataa
     3721 ctgaatttag tactcgaagg tatacctgta ttccgttgta tttcccgctc cgtcggtagc
     3781 cacaaaaaca agttcctgtg ttttgccttt ttccagcctt gaatcgtcga acaggtaagt
     3841 gtaaagggag gatttcatgt cgtgtgaaaa gaggacgaat ttcccgttaa tagttccttt
     3901 aaaggcggat attccgcttt tgttatccga tagccggatt tggatcctgc gtgaagccac
     3961 ccatttttcc ggatttacgg ggacgatatt cggtgggaaa gtgtctgaat caacggcata
     4021 catcctgccc aactcgcgga tggaaacctc catgccgttc cgtttgtaag taccgcctat
     4081 ccagttgtca ttgcctgttt ccgtaatttc cacaataccg tattgctgct tgttatccag
     4141 tgtgtccacg tttaatttta tccatacggt agctttttgg tgtagcggta ccggtgagtc
     4201 attcacccgg tgtatatcgg agtaatagag cgttgagcca gttttacggt gtgaaaaaca
     4261 aaagctattg tatagatttc cagaaggtat gtcgagcata aaatcaaagt caacaaatgt
     4321 attgtgtagt gtccacggca taaagttttt gcaggaatcc gtttttgcga cgggttgttg
     4381 ctgtcctacg accacaaaag agtatacaag ggtatttccg ttataatctt caagttcata
     4441 gcgcatgcgg taatctcgct cttcgttgat atcgataaat ccgttattga ctgcgtcgta
     4501 gaacttgagc gtgtttcccg gctctataaa tgacttcatg taaaaagaac ttcgttcccg
     4561 ccattcttca aagtcgataa atgaattcaa catgcgcgtc ttactgaaag gaaacctgtc
     4621 gatggaacta ctgaaaacct gcttttcgtc tacaaataac ctgacgtgtt tgacaccgta
     4681 gatgttggat tgaccgttca tccgatcgta tgcttttacg gctacaccga ttctacccca
     4741 tgcgtttatc gcttgaggcg gtgccagggg tatcccggac ttgtttttca aaatattcat
     4801 ccgtagggga ttgctgcttc cgttcacgac tccttttccc tcaatagtat aaaacgcaat
     4861 gccacgaatc tcgggctttt gagtgtcgag gatggcccgg ctgaaaaaag aaagcggatc
     4921 cagtgggtcc tgcgtctggg tatcgcgcac ttcaaagtgc aggtgtgggc caccggaact
     4981 gcctgtattt ccactcaatc ctatttgctg accttttttt acgatcagca aacccggttc
     5041 ggggtaaagc tccacccggt aactttcttg ctcgtattgc ttttctttca caaaatcggc
     5101 aatagggctg gaaaaactgt tcaggtgcgc ataaacgctg gtatgtccgc tgggatggtc
     5161 gatgtagagt gccaacccat atcctccagg ggaaacactt atgcgcgaaa tatagccgtc
     5221 ttcgatggcc acaaccggtt tgtttaccgt ttgttgtgtt ttgaaatcaa tgcctgaatg
     5281 aaagtggttg cttcgcaact ccccgaagtt ggcactgagt gccggaggaa tatcaagcgg
     5341 gtcggtatag ggatgttgtg tataagccga gaaacgagcc atggaaaaga aaagaaaaac
     5401 gcatgaaaat agtatattga acttcataaa tccttgtttg atttgcaaac ataggtataa
     5461 aagagtaaaa caacaatagg aattccgcat acaatcgatt cataatccgt tttgaagaac
     5521 ggagcataca ctctaaatgc cgcattttta cgaggagtag gcgaggatag cgtcaggtag
     5581 ggttattttg ttaaataaaa ttaaaataaa gaaattcatt ggtactttgc attgcatatt
     5641 accttgttta atttatcttt gtggtgtaaa taaaaattta ttgaaaaatg aatattgaaa
     5701 atacccaaag tcaaatgagg aaaggagtac tggagtactg cattttggcg attatcaggc
     5761 ggaacgaagc atatccgggt gatataatcg acgaaatgaa aaatgcaggc ctgcagcttc
     5821 ttgaaggaac gctatatcct ttgctcaatc ggttgaagaa cgccgaaata ctcacttatc
     5881 gctgggtgga aagtacttcg ggccctcctc gaaaatattt tatcctgaca gagaaaggtg
     5941 aaaaatttta tgatctttta gaatcgacat ggatagaatt atccaccgga gtaaacgtcg
     6001 ttgtcaacgg caattattct aaagaagaca ctgttgtcaa tgacaattta aatgaaccta
     6061 acaccacaga acaatgaaaa aagtgattaa tatcaatttc caggggcagg tcatcgctat
     6121 cgaagaaact gcttatgaac ttttgaagca atacatagac agtctgaaaa aatatttttc
     6181 gcgggaagag ggcggagaag agattgtaaa cgatatagaa aaccgtattg ccgaattgtt
     6241 tggaaaccgg ctgaagctgg gtatcaattg cattaccgat gaagatgttc agtccattat
     6301 ctccagcata ggaagaccgg aagattttga cacggaatat caggagtcgg tttacgccta
     6361 tggtgaaaca ccttttgaac agtttgcctc aacttcggag cagccgaaat cggaacctga
     6421 cggaagcgaa aggcggcaga ccctttaccg caatgcaaac gataaaataa ttgctggtgt
     6481 gtgcagcggc ctcgctcatt acttcaaaat agatccggtg tggatgcgta ttgtttttgt
     6541 tgtgcttttc agtgtgttgt tttgggtgta cctcatttta tggattgtct taaagcccaa
     6601 agtgctggag acaaatgttg ccagaaggct ttatcggaat cccaatgacc gtttcctggg
     6661 aggggtttgc ggaggaattg cagcgtactt caaaattgac acgtggattc cgcgtttgct
     6721 ctttgcagct ccgttatttc tgaatttgat ggggatgatc tccatacctt ttttcccgtg
     6781 gaaccgtttt tttgacaacg tggatttcaa ctggaacatc aatatgtcgg ttgtcgtgat
     6841 ctatgccgtt ctatgggtaa ttattccgag ggccactacc gtaaagcaga aactcgaaat
     6901 gatgggagag gaggaatata tcaaatccat acgtgaaact gtaagcgata acgtggcaag
     6961 tgtgaaaagc aaaactgacg acggtgataa ttataccaag ccgtacgctg caacagctgc
     7021 tgttgccggt gattcggaaa aagtgtctct ggatgccatg ccgcccgagc ctccacggtt
     7081 ccatgccgcg aaagcgtcag caaacgcaac ggctggttcc ggtaatacgg gttgtctgaa
     7141 tgctttggga atccttttca aaattatatt ctttgcgttc gccggaattt ttgctgtagt
     7201 tacgacggga atgatgatcg cttttcttgt agccggcaca aaatttgtcc ctctaaagtc
     7261 gttatttatt gacctgggtt acgaaaatac cctgttgtgg ttgtctgcag gattactgtt
     7321 tgcagttcct attatatcca ttatcgtgtg gatcgttcgt cggtcaatga aagcaaaatc
     7381 acgtccggtt atcggagtcg tgtcgattat cttgtgggtc gtcggaatct tttctgctac
     7441 tatgctcgga tttaaagtgg ccgaaaaatt tagtgtggag tcatctgctg aaaatgtaca
     7501 ggctctttct gcttttagcg gcgacaagtt atacgtggat atggcggttt atccgtcaga
     7561 ctactactct ttcagccgta acttcggacc gggttcagat ttggataatt tcccctatta
     7621 taccataaat gaggactccc tgctgttttc aaacattaag ctacagattg ttcaaagcca
     7681 ggattctttg tattacgtga gaaccatttc gtcaagttcg gaaagagacc tgaagattgc
     7741 tagaaagaat gccggagagt ttacttatcc cttacaacaa caagattccc tgttgttttt
     7801 gcccgagttt ttctcggcac ctatttcaca aggtttccgc ttgcagggga tgatcgtgga
     7861 aatagctgtg ccgcttggca aaaaaattga gatcgacgaa cggttggacg attacgataa
     7921 ttttacctca aattcgtctg ttcgccgaaa attgaaaaaa agccgcaaca acaagacttt
     7981 cgattgggat tatggggaag aatatattct cgaaaacgga ggtttaaaca agacggtcat
     8041 acttacggat tctatataag ttttactttt gcattaataa cttcatgtgt tcaaaagcag
     8101 ccggacgttt tttccggctg tttttttgtt gaaaaagagg agggattttc ctttttttgt
     8161 agttttgtaa aaacaaccgc atagctatgg agaaacaagt ccggaaaatc gttacaattt
     8221 ttaatcttcg ttcagagttg gaggattacg aaaccattca tgcagaaata gaaaaaggga
     8281 ttcagttcag ggggaccaac ctctggatcc tgatgtttgc cattgtggta gcatcggtgg
     8341 gattgaacat gaactccacg gctgtaatta tcggcgctat gcttatctct ccgttgatgg
     8401 gtccaataaa cgggatgggg tatagtttgg caacttacga tttttatctt ttcaggcggt
     8461 cgcttaaaaa ctttatctat gctgtagggg taagcctgct cacctccgca ctctattttt
     8521 tcctttctcc gataaatgaa gctcattcgg aattgttggc ccggaccagc cccactatct
     8581 acgatgtttt gattgctctt ttcggtgggt tagccg
//
LOCUS       DAOSDR010000081         8569 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrimonas sp. isolate AMR_MDS_1826
            NODE_3079_length_8569_cov_4.088208, whole genome shotgun sequence.
ACCESSION   DAOSDR010000081 DAOSDR010000000
VERSION     DAOSDR010000081.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863355
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrimonas sp. (wastewater metagenome)
  ORGANISM  Petrimonas sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Dysgonomonadaceae; Petrimonas.
REFERENCE   1  (bases 1 to 8569)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8569)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:10:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,720
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 35
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8569
                     /organism="Petrimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3079_length_8569_cov_4.088208"
                     /isolate="AMR_MDS_1826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2023866"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(1090..1683)
                     /locus_tag="PLI30_04690"
     CDS             complement(1090..1683)
                     /locus_tag="PLI30_04690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007658568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOI78886.1"
                     /translation="MEVLLSMTDEELVVSYAEGNNCAFEILLSRHKQSLFSYILYRVR
                     NRDLADDIFQDTFIKAILTIKQGRYTENGKFRAWITRIAHNLIVDCFRQERNENTVSN
                     DEYEFDLFNNFRLCEDNIEVTMVKAQVFDDVRRLVEYLPDTQREVLNMRYYQELSFKE
                     IADKTGVSINTALGRMRYAILNMRKIADDNRMILSMS"
     gene            complement(1881..3599)
                     /locus_tag="PLI30_04695"
     CDS             complement(1881..3599)
                     /locus_tag="PLI30_04695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019540479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Acyl-CoA dehydrogenase C-terminal
                     domain-containing protein"
                     /protein_id="HOI78887.1"
                     /translation="MANFYTDTPQFRHYLNHPLMKRIVELKERNYADKYTYDYAPMDF
                     EDAMDSYDKILEVVGEICGDIIEPNAETVDHSGPTVADGRVTYATPTQENLEALNKAE
                     LMGMAFPRRFGGLNFPMVPYMISADIVSRADASFQNIWMLQDCGETIYEFASDEQKNE
                     YLPRVAKGETMSMDLTEPDAGSDLQAVMLKASYNEKEGQWYLNGVKRFITNGDADIHL
                     VLARSEEGTKDARGLSMYVYDKNSGGVTVRRIENKMGIKGAPTCELVFKNAKAELVGS
                     RRMGLIKYVMSLMNGARLGIMAQSVGISEAACREAYNYALERRQFGKAIIEMPPVFEM
                     LANMRAKTDASRTILYETCRFVDMYKILEDISRERKLTPEERDEMKYYSRLADAFTPL
                     GKGMTSEYANQNAYDAIQIHGGSGYMKDYKCERLYRDARITNIYEGTTQLQVVAAIRH
                     VTTGTYLNRIREYEAMPVLPELEPLKRTLSKMAQMYEKLVEIVTAPKDEEYLDFHARR
                     LVESAGHVIMGHLLLQDANKEPEMFRRSAEVYIHYGQIEVVKNYNFVTKSRIEDLGYY
                     KPALSE"
     gene            complement(3606..4625)
                     /locus_tag="PLI30_04700"
     CDS             complement(3606..4625)
                     /locus_tag="PLI30_04700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007486135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HOI78888.1"
                     /translation="MNNVFVYLEIEDGTVAEVSLELLTKGRTLASQLGCRLEAIAAGD
                     KLDTIEKQVFPYGADVLHIFKDKRLSPYTTLPHTSILVNLFKKEKPQICLMGATIIGR
                     DLGPRVSSALGSGLTADCTSLEIGDHEEKKVGKVYENLLYQIRPAFGGNIVAWIINPD
                     HRPQMATVREGVMKKEIADPNYKGTVVEHDVKDYVSPDDFVVSIIDRHVEKSKVNIKN
                     SPIIISGGYGVGSKENFQLLYDLANVLGAEVGASRAAVDAGYAEHERQIGQTGVTVRP
                     KLYIACGISGQIQHIAGMQESSLIISINNDPSAPINAIADYVITGDIEKVIPKLIKYY
                     KKNSK"
     gene            complement(4630..5502)
                     /locus_tag="PLI30_04705"
     CDS             complement(4630..5502)
                     /locus_tag="PLI30_04705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010539018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HOI78889.1"
                     /translation="MAFNIIVLAKQVPDTRNVGKDAMKADGTVNRGALPAIFNPEDLN
                     ALEQALGIKDQYPGTKITVLTMGPARAAEILREGLFRGADDGVLLTDRAFAGADTLAT
                     SYALSMAVKQMGNFDMIISGRQAIDGDTAQVGPQVAEKLGIPQITYAEAIESIDKKKI
                     RVKRRLENGVEVVEAPLPLLVTVNGSARICRPRNAKFLQKYKHAKTVTERQTENIDYI
                     DLYNARPYLNLTEWSVADVNADPVQCGLSGSPTKVKKIENVVFQAKESKRLTDDDTEL
                     EDLIKELIANHTIG"
     gene            complement(5543..5731)
                     /locus_tag="PLI30_04710"
     CDS             complement(5543..5731)
                     /locus_tag="PLI30_04710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI78890.1"
                     /translation="MKDQIAYFKPIQEFNGCDYWIQKKEIGLMMFVLDLIFTQKTLFC
                     AIFSIVVSNFVGEAEFFL"
     gene            5938..6987
                     /locus_tag="PLI30_04715"
     CDS             5938..6987
                     /locus_tag="PLI30_04715"
                     /inference="COORDINATES: protein motif:HMM:NF040069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6340 family protein"
                     /protein_id="HOI78891.1"
                     /translation="MKHLVFSGILFFSILFFSNCAGIQYMSIETREPAQVTLPTEVKS
                     VLVVNNVVQQPDEIGHNIKRLGKKQSDRIKVSADSVAIFYTEALSQFLGEEEYFNAVK
                     YYQKPLRSDNDFWQEVPITPETMHELRNATTTDAIISLDKLILQTDRTDFFRQEGYNY
                     AGLTGRIQSVIRVYLPSMEGKIPVVQFTDSMKWEGYDIHDGRAYADLIVPSQEEAMKQ
                     LAVFAAEKMTKVFSPHWENQDRWYYTQMSSKMREGEVFAKNNQWFNAIEKWNEFYTKE
                     KNKLNQAKAAHNIALGYEMLDDMDSAYKWAVLAVDLFLQSTTSGSLERRRSLLFKNEI
                     ERRRNTSNKLNMQIK"
     gene            7265..>8569
                     /locus_tag="PLI30_04720"
     CDS             7265..>8569
                     /locus_tag="PLI30_04720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019540473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rho termination factor N-terminal
                     domain-containing protein"
                     /protein_id="HOI78892.1"
                     /translation="MAYNIIELNEKLTTELRALAKEMGIRRPDAYKKEELIYKILDEQ
                     AIAGTKNLSPAPHPRPNNGRKTIENKNNTQKQEVKAETGRPAEKDRKPVTEVEVKEKE
                     KEKEKEKEKEKEKEQEKPKPEVKTLTRVPKENPVQNNKQVQSTNKPHEVKKQEPKQHA
                     VVVSAHPAIIPDAVVTDSAPVDQPKAEATPTPPVKPTQLVFRSSKHRERERISQVPPV
                     TLPPVVPEEPIEDALKTEVMEKPVVIEKKASLLQELITPAPIQPRQQQPQQSKNINQQ
                     QTVVQQKEPAYEFEGILNASGVLEIMSDGYGFLRSSDYNYMSSPDDIYVSQSQIRLFG
                     LKTGDVVEGPIRPPKEGEKFFPLVKVDKINGRKPEEVRDRVPFDHLKPLFPDQKFNLT
                     KGYNDNLSCRVVDMFSPIGKGQRGLIVAQPKTGKTMLLKDIAN"
BASE COUNT         2529 a         1885 c         1742 g         2413 t
ORIGIN      
        1 agaagttgtt tataattatg aagaaaaaaa tttgcaccgt atttatgtaa tattgtatat
       61 ttgcgatact caacattcag gaaaatttta gtacgaaaat cgtacaaaaa aatatgagga
      121 gttctttggc attttgtaca caataaaaag attaggatcg gcaggatgtg tagcaccaca
      181 attcgacgca acttgtaaca tttccttgat aaaggccttc caaaagaatg cttttgcaac
      241 gaaatccggt cgtaattctc gtattccaac ccatttaccg ggctttctgc agagcatccc
      301 gaaagattca catcgaatct ggacagaatg taaagtagag cacaagcgcg ataaatgatt
      361 ttgtagaatt ttacggacga tgctcgggat gaacttcccg ggtttgcaat gtcaagacga
      421 gaaatgaacg tgtttaacga atcgggaaaa atgatttgaa agaaattttg aaaaatttgt
      481 tccggattgg atgagcatat tctgtgggtt tttacccatt caaccgttaa gtgtctccca
      541 caagatggtt gttaaagaat tgaagcattc aggctacaac acaggtttgg aaacaaacca
      601 cttcaccctc gtttgttccc gtttcaaacc ttatggatat ttacttccgt gaggcagaaa
      661 acagaaaaag ttgcacacag gcatcgacgt gtttttcaga tctttcggaa ttaccgtttt
      721 gaaaaagacg gatcaagaga tcggtttttt ggtaatacca ttccaaactg accggttttc
      781 tttttgtgct tcgaagtttg aagacgctat ttgttgactt ttgatacaac aatttcaaaa
      841 agaggctaat caacaactta gggttgcatt tatggtttca cactgtttct gacgctttag
      901 gttgtattca tgttttgaag tataatctac attccgaaat taaacttccc gtttggacag
      961 tctgaagaca gattctacag gataagttga agtacagttg tgtataggat acttagaact
     1021 ccttcctaaa caaaaaaggc cggcttaaat accggtcttt ttttgtttgt atacgtttgt
     1081 cctgaagacc tagctcatcg ataatatcat tcggttatcg tctgctattt tacgcatatt
     1141 caaaatagca tatcgcattc tccccaatgc ggtgttgatg ctcacaccgg ttttatcggc
     1201 aatctcttta aaactcagtt cctgataata acgcatattc aatacttcac gttgcgtgtc
     1261 cggcaggtat tcaaccagcc tcctcacatc atcaaacacc tgcgctttta ccatagttac
     1321 ctcaatgtta tcctcacaga gcctgaaatt gttgaacagg tcaaactcgt attcatcgtt
     1381 tgagaccgta ttctcgttcc gttcctgacg aaaacaatcg acaatcaagt tgtgtgcaat
     1441 gcgtgttatc caggcacgaa atttaccgtt ctcggtatac ctgccctgct ttatcgtgag
     1501 aatagccttg atgaacgtgt cctgaaaaat atcgtcagcc agatcacgat tgcgaaccct
     1561 atagaggatg taactgaaaa gggattgttt gtgacgggag agaagaatct caaatgcaca
     1621 attgttacct tcggcgtaag aaacgaccaa ctcctcgtcg gtcatcgaca aaagaacctc
     1681 cattactaaa actattaaac gatttatttt taaattagtt gagtaatgtc actccgatag
     1741 gcagataatt ttaaaaaaag aatttgttaa tatgagttga acaaagaaaa ttacaatttt
     1801 cataataaac aaaaattgaa tgttaaaaaa aacggaaagg ttcaattctt tccgtttcgc
     1861 aattacctga aaagtaactt ttattcgctt aaagccggtt tataataacc caggtcttcg
     1921 attcgagatt tggtaacaaa gttatagttc ttcactactt caatttgtcc gtaatggata
     1981 taaacctcgg cactgcgacg gaacatttcg ggttctttgt tggcatcctg caagaggagg
     2041 tgtcccataa tcacgtgtcc ggcactttca accaaacggc gcgcatgaaa atcgaggtat
     2101 tcttcgtcct tgggtgcggt aacaatttca accaattttt cgtacatctg cgccattttc
     2161 gataatgtac gctttaacgg ctccaattcc ggcaatacgg gcatagcctc atattccctg
     2221 atccggttca ggtaagttcc tgtggtgacg tgtcgaatgg cggcaacgac ctgcaattgc
     2281 gtagtaccct cgtaaatatt ggtaatacgg gcatcacgat atagccgttc acacttatag
     2341 tctttcatgt acccggatcc accgtgtatc tgaatggcat cataggcatt ctggttcgca
     2401 tattcactgg tcattccttt tccaagcggc gtgaaagcat ccgccagccg cgaatagtac
     2461 ttcatttcgt cacgttcttc aggagtaagt ttgcgctctc tggaaatatc ttccaatatc
     2521 ttatacatat ccacaaaacg acaggtttcg taaagtatgg tacgtgacgc atcggtttta
     2581 gcgcgcatgt tggctagcat ctcgaaaaca ggtggcatct cgataatggc tttcccaaac
     2641 tgacggcgtt ccagtgcgta gttgtacgct tcgcgacaag cagcttcgga tattcctacc
     2701 gattgagcca tgatacccag gcgggcaccg ttcatcaacg acatgacgta cttgatcagt
     2761 cccatccggc gcgaacccac taactcggct ttggcatttt taaaaaccag ctcgcaggtt
     2821 ggcgcccctt ttatacccat tttattttca atacggcgta cggttacgcc tccgctgttt
     2881 ttgtcgtaaa catacatcga taaaccgcgt gcatcttttg ttccctcttc cgaacgggcc
     2941 agcaccagat gaatatcggc atccccgttg gtaataaatc gtttcactcc gttcaggtac
     3001 cactgtcctt ccttttcgtt atacgatgct ttcagcatca cggcttgcaa atccgaaccg
     3061 gcatcgggtt cggtcaggtc catcgacatg gtctcccctt tggcaaccct cggcaggtat
     3121 tcgttttttt gctcgtccga agcaaactcg taaattgttt cgccacagtc ttgcaacatc
     3181 cagatatttt ggaaactggc atccgccctg ctcacgatat cggctgaaat catgtaaggc
     3241 accataggaa aattcaatcc tccaaaacgt cttggaaaag ccatccccat cagttcggcc
     3301 ttgttcaggg cctccaggtt ctcttgggta ggtgtagcat acgttactcg tccgtcggcc
     3361 actgttggtc cgctgtggtc taccgtttcg gcattcggtt cgataatatc accgcaaatc
     3421 tcgcccacta cttccagaat tttgtcgtag ctgtccatag cgtcttcaaa atccatcgga
     3481 gcgtaatcgt aagtatattt atctgcgtaa tttctttctt tgagttccac aattcgtttc
     3541 atcagtggat ggttcagata gtgtctgaat tgcggcgtgt cggtataaaa gtttgccatt
     3601 tcttcttatt ttgaattttt cttataatac ttgatcaatt tcggaattac cttttcgata
     3661 tcgccggtaa tgacgtaatc agcaatagca ttaatggggg cactagggtc gttattgatg
     3721 gaaatgatca gcgaactctc ttgcattccg gcaatgtgtt gaatctgtcc cgaaataccg
     3781 caagcgatat acagtttagg gcgtacggta acaccggtct gtccgatttg acgttcgtgt
     3841 tcggcatatc cggcatcaac ggcggcacgt gatgccccta cttcagctcc caaaacattt
     3901 gccaaatcat acaacaactg aaaattttct ttcgaaccta caccgtatcc cccggatata
     3961 atgatgggcg aatttttgat attgactttc gatttttcca cgtgacggtc gatgatggaa
     4021 accacaaaat catccggaga aacatagtct ttcacatcgt gctctaccac cgtccctttg
     4081 taattcggat cggcaatttc ctttttcatc acaccttcgc gaacagtcgc catttgcgga
     4141 cgatggtcgg gattgatgat ccacgctaca atatttccac cgaaagctgg acgaatctga
     4201 taaagcaggt tttcgtaaac tttgcccact tttttctctt cgtgatcgcc aatttcaagc
     4261 gatgtgcaat cggccgttaa gccgcttcct aatgccgatg atacgcgcgg gcccaaatca
     4321 cgcccgatga tggtagcccc catcaggcaa atttgcggtt tttctttttt gaacagattc
     4381 accaggatgg atgtgtgcgg caaggtagta tatggcgaca agcgtttgtc tttaaaaata
     4441 tggagaacat ctgcaccgta tggaaaaacc tgtttctcaa tggtgtcgag cttgtctcct
     4501 gcggcaatag cttcgagtct gcaacccagc tggctggcca gagtgcgtcc cttggtaagt
     4561 agttccaggc tcacttctgc aaccgtgccg tcttctattt cgaggtatac gaatacgtta
     4621 ttcattgttt taaccaattg tgtgattagc tattaattct tttatcaagt cttccagttc
     4681 ggtatcgtca tcggtcagtc gtttgctttc ctttgcctga aatacaacgt tctctatttt
     4741 ctttactttg gttggagaac ccgataatcc gcattgaacc ggatcagcat ttacatcagc
     4801 gacactccat tccgttaaat tgaggtatgg gcgtgcattg tacaaatcga tataatcgat
     4861 attttcagtt tgacgttcgg taacggtttt agcgtgtttg tatttttgca gaaattttgc
     4921 gttacgcgga cggcaaatcc tggccgatcc gtttacagtg accaataacg gcaggggggc
     4981 ttccactacc tctaccccat tttcgagacg acgtttcacg cggattttct tcttatcgat
     5041 gctctcgatt gcctcagcat acgtaatttg aggaatgccc aacttttctg ccacctgtgg
     5101 cccaacctgc gcagtatcgc catctatagc ttgccggccg gagataatca tatcaaaatt
     5161 acccatctgc ttaacggcca tcgacaaggc ataagatgta gccaaagtgt cagcgccggc
     5221 aaaagcacga tccgttagaa gcacaccgtc gtcagcacct ctgaataatc cttcccgaag
     5281 gatttctgca gcgcgggcag gccccatggt tagtacggta atctttgtcc cgggatattg
     5341 atctttgatt ccaagagctt gctccaacgc attcaaatct tccggattaa aaatagccgg
     5401 cagcgctcct ctgttaactg ttccgtcggc tttcatcgca tctttcccaa cgttgcgcgt
     5461 atcgggaacc tgctttgcca acacaataat gttaaatgcc atttcctata tgtttattta
     5521 tgttcttttg gagacggcaa atttacagaa agaattcagc ttcaccaaca aaatttgaaa
     5581 caactatcga aaaaattgca caaaaaagtg ttttttgtgt aaaaattaaa tccaatacaa
     5641 acatcattaa acctatttct ttcttttgta tccaataatc gcagccgttg aattcttgaa
     5701 tcggtttaaa ataagctatt tgatctttca tgtcattttt ttaatggtgt ttaattttta
     5761 tttcgcatcg taatggaaag tggtttaaaa aaaatgaaag ttcagcggta taaaaaagta
     5821 aaaaacaaca taaaagaata aaacaaaatc atatcattcg taaaatacaa aacaatttct
     5881 aaataaattt gtaattttga ctcataaaat ctcaacctat tcagaccaaa tttgtctatg
     5941 aaacatttag ttttctctgg tatattgttt ttttccatcc tgtttttcag taattgtgcc
     6001 ggtattcagt acatgtctat cgagacgcgc gaaccggcgc aggttactct tccaacggaa
     6061 gtgaagtcgg tattggtggt aaacaacgtg gttcagcagc ctgatgaaat tggacataac
     6121 atcaaaagac tgggaaaaaa gcaatccgac agaataaaag tatctgcgga cagtgtggct
     6181 attttttata cggaagcatt gtctcaattt ttgggtgaag aagagtattt taatgctgtt
     6241 aaatattacc agaaaccgct gagaagtgat aatgatttct ggcaggaagt tcccataacg
     6301 cccgaaacga tgcatgaact gcggaatgca actaccactg acgcaatcat atcgctggac
     6361 aaactgattt tacaaaccga cagaaccgac tttttccgac aggaagggta caattatgct
     6421 ggtttaaccg ggagaataca atcggtaatt cgtgtgtatc tcccatcgat ggaaggtaaa
     6481 atcccggtgg ttcagtttac cgacagcatg aaatgggaag gatatgatat tcatgacggt
     6541 cgtgcttacg ccgacttgat tgtcccttca caagaagaag caatgaaaca gcttgccgtg
     6601 tttgcagcgg aaaaaatgac gaaagtattt tcgccgcact gggagaatca agaccgctgg
     6661 tactacacac aaatgagttc caagatgcgt gaaggagaag tctttgccaa gaataatcag
     6721 tggttcaatg ccattgaaaa atggaacgaa ttttacacaa aggaaaaaaa caagctaaac
     6781 caggcaaaag ctgcacacaa tatagcactg ggttacgaaa tgcttgatga tatggacagc
     6841 gcgtataagt gggccgtctt agcagtcgac ctctttctgc aatcaaccac aagcggttcg
     6901 ttggaaagac ggcggtcgct cctgtttaaa aatgaaatag aacgtcgaag aaacacatcc
     6961 aataagttga acatgcagat aaaatgaaaa aaattcctgt tggcgaactg cttaatggga
     7021 atttttagta aaaatatatc ttcctttttc ctttgttttc aaaaaaaaca ttacctttgc
     7081 accagaatct tcaaagataa gtgaatatat tctacggata ttcactcaac aattcaaaat
     7141 aaaaccatat tgagtttttt aatcattgtt cggaatagcc ttgtttcctt caaacattat
     7201 atataaaaac tcagtttcaa tcattaaaat ataaaaccta aacgtttttt taacaaataa
     7261 ttaaatggct tataacatta ttgagcttaa cgaaaagcta accacagaat tacgagctct
     7321 tgccaaagaa atgggtatac gaagacccga tgcgtataaa aaagaagagt taatctacaa
     7381 aatccttgat gagcaagcta tcgccggaac aaaaaatctg agcccagccc cgcatcccag
     7441 accaaataac gggagaaaaa caattgagaa caaaaacaac acgcagaagc aagaagtcaa
     7501 agcagagaca ggccgcccag cagagaaaga tagaaagcct gtaacagaag tagaagtaaa
     7561 ggaaaaggaa aaggaaaagg aaaaggagaa ggagaaggag aaggagaagg aacaagaaaa
     7621 gccaaaaccg gaggtaaaaa ctctaacccg ggttccaaaa gaaaaccctg tccaaaacaa
     7681 caagcaagtt caaagtacaa acaaaccgca tgaagttaaa aaacaggaac caaaacagca
     7741 cgctgtcgta gtttccgcac atcctgcaat cattccggat gcggttgtaa cggatagtgc
     7801 tccggtggat cagccaaaag ctgaagcgac acctactcct cccgtaaaac cgacacagct
     7861 tgtctttcgt tcatccaaac accgtgaaag agaacgaatc tcacaggttc caccggtaac
     7921 tttaccaccg gtcgttcctg aggaacccat agaagatgct ctcaaaacag aagtgatgga
     7981 aaaaccggtt gttattgaaa agaaagcatc tcttttgcaa gaacttatta ctccagcacc
     8041 tatccagccc cgccagcagc aaccgcaaca aagcaagaat ataaatcagc aacaaaccgt
     8101 tgtacagcaa aaggaacctg cttacgaatt cgaaggaatt ttgaatgcat cgggtgtatt
     8161 ggagatcatg agtgacggct atggcttctt gcgttcttcg gactataact acatgtcttc
     8221 accggacgac atttatgttt cccaatctca gatccgcctg tttggcctga agacagggga
     8281 tgtggtagag ggtccgatcc gtccgccaaa agaaggagaa aaattctttc ctctggtaaa
     8341 agtagacaag ataaacggaa gaaagcctga ggaagtacgc gaccgtgtac ctttcgacca
     8401 cctgaaaccg ttgtttccgg atcagaaatt caacctcacc aaggggtata atgataatct
     8461 gtcgtgcagg gtagtcgaca tgttttcgcc catcggcaag ggacaacgag gcctgattgt
     8521 cgctcagccc aaaacgggta agaccatgtt gctcaaggat attgccaat
//
LOCUS       DAOSDR010000082         8414 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrimonas sp. isolate AMR_MDS_1826
            NODE_3147_length_8414_cov_3.615624, whole genome shotgun sequence.
ACCESSION   DAOSDR010000082 DAOSDR010000000
VERSION     DAOSDR010000082.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863355
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrimonas sp. (wastewater metagenome)
  ORGANISM  Petrimonas sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Dysgonomonadaceae; Petrimonas.
REFERENCE   1  (bases 1 to 8414)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8414)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:10:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,720
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 35
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8414
                     /organism="Petrimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3147_length_8414_cov_3.615624"
                     /isolate="AMR_MDS_1826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2023866"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            376..1944
                     /locus_tag="PLI30_04725"
     CDS             376..1944
                     /locus_tag="PLI30_04725"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HOI78893.1"
                     /translation="MKRTISSVFFFWVITSFFFVEAQHARIGVISDLHYTHPALIVEK
                     GKALEDYLRRDRKLLLESDALLRKSVENLLCEDVNVVLIPGDLTKDGELVSHRGVVEL
                     LSPLREKGVKVLVVPGNHDIDNPDAVSFHGGETHKVESVTPAEFKDIYSDYGYDSALS
                     LDEHSLSYVNEPVEGLRIICIDACKYRDNTFIYRGAKKDSCVTHGTVKPETMNWIKNE
                     VRLAKLLGKQVIAMMHHGVVEHFDHQSFFASPYLVDNFEWVQQSFMEAGLNVVFTGHF
                     HASDIARIEDEKGNYLYDIETGSAVTYLCPYRVIDLSNNHLDIQTKLTDSIDFPIPSG
                     MNFQAYAKKMVDVGFNEMISALISEYHSSLPVYLPRWLKGIIRIPDAEKFTDMVLFNL
                     SPSAVEMLVAHYGGNENNIENAHRHKEELLLAIDSFVRDLCKASAGSLSGIIEKLVVR
                     SKTIKKTKQAVSSIWENTFLTGKGVVKGRLMDNSINDLNYLLTLNTNENINNGNFYAN
                     ENVNDEVEVKLFSE"
     gene            complement(2101..2562)
                     /gene="nrdG"
                     /locus_tag="PLI30_04730"
     CDS             complement(2101..2562)
                     /gene="nrdG"
                     /locus_tag="PLI30_04730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004328836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008998 - ribonucleoside-triphosphate
                     reductase activity [Evidence IEA];
                     GO_function: GO:0043364 - glycyl-radical enzyme activating
                     activity [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009265 - 2'-deoxyribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HOI78894.1"
                     /translation="MLKYTDYDIVFQEIPDEVTLAVNLSGCPHRCKGCHSPHLQQDIG
                     EELNEGALSRLLQLYEHSITCICFMGGDAHAEEVCQLANHIRMGWKGKLKTAWYSGNS
                     NLHPMATGRFDYVKTGPYLEALGGLNKKTTNQRLYRFTGGSFKDITAEMQK"
     gene            complement(2664..4793)
                     /gene="nrdD"
                     /locus_tag="PLI30_04735"
     CDS             complement(2664..4793)
                     /gene="nrdD"
                     /locus_tag="PLI30_04735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019540600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HOI78895.1"
                     /translation="MGNKEIFIVKRDGKKVPFSIEKIRNAVAKAFLAVGSFATEEDFT
                     TLLSHLHISDGMTVEEIQNQVETALMREKYFAVAKAYMLYRQKHTETREIQSKMNFLM
                     NYCNAQNAATGSKFDANANVEKKNIATLIGELPKKNFIDLNRKMITDRIKEMYGKELA
                     DKYIGMLNEHFIYKNDETSLANYCASITMYPWLLNGTLPIGGNSMPPTNLKSFCGGFI
                     NMVFIVSSMLSGACATPEFLMYLDYFIRKEYGDDYFGRSGDVVDLSRRQRTIDKMITD
                     CFEQIVYSINQPTGARNFQAVFWNISYYDRLYFESLFGEFLFPDGSRPVWESLSWLQK
                     RFMKWFNKERTKTVLTFPVETMALLSREEDVIDTEYADFTAEMYAEGHSFFTYMSDNA
                     DSLSSCCRLRNKIQDNGFSYTLGAGGVSTGSKSVLTINLNRCVQFAKRTGQPYSTFLE
                     EVVDLTHKVQTAYNENLKMFQQKGMLPLFDAGYINLDRQYLTIGVNGLVEAAESLGIA
                     INHNPQYKAFVQEVLGIIERYNKKYRSRELMFNCEMIPAENVGVKHAKWDREDGYFVP
                     RDCYNSYFYRVEDPSLNVIDKFRLHGRDYIEHLTGGSALHLNLEEHLSKAQYRQLLRV
                     AAHEGCNYFTFNIPNTVCNDCGVIDKRFLKECPGCRSLNVDYLTRIIGYMKRVSNFSA
                     ARQAEAERRHYGKPDTTEKPKEEPSIY"
     regulatory      complement(4914..5128)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            5459..6982
                     /gene="purH"
                     /locus_tag="PLI30_04740"
     CDS             5459..6982
                     /gene="purH"
                     /locus_tag="PLI30_04740"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019539747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HOI78896.1"
                     /translation="MSKEINSALISVYHKDGLEEILKLLNSLNVKLISTGGTRDFIQS
                     LGYECESVEEVTGYPSILGGRVKTLHPKVFGGILYRRDEEQDRKIIKSYDIPSIDLVI
                     VDLYPFEETVATQAPEDEIIEKIDIGGISLIRGAAKNFKDVIVVASRKQYKPLLDLLK
                     EKSGNTDIEDRRWFAKEAFAVSSGYDSAIFNYFDKGESPVLRISADSGKTLRYGENPH
                     QKGVFYGNFNEIFEQLHGKEISYNNLLDIDAAVSLIADFDETALAILKHNNACGMACR
                     NTVKEAWDAALAADPISAFGGIVVTNRQVDADAAKVINEIFFEVIIAPDYSQDALNIL
                     FQKKNRIILKQKITGKEANNTQVRSALNGFLVQDKDTSIQTRSDLTVVTAKVPTEKET
                     EDLLFANILVKHTKSNAIVLAKNKQLIASGTGQTSRVDALNQAIEKAGTFKFNLNGAV
                     MASDAFFPFPDCVEIAHKAGITAVVQPGGSIKDAESVAYCNENGLAMVTTGIRHFKH"
     gene            7049..8074
                     /locus_tag="PLI30_04745"
     CDS             7049..8074
                     /locus_tag="PLI30_04745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004304915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HOI78897.1"
                     /translation="MGLFSFTQELAMDLGTANSIIVNNAGKILLDEPSIVALDRKTDR
                     MIALGEKARQMHGKTHENIHTVRPLRDGVIADFNAAEQMIRGMIKMATKNKGGLFSPS
                     LRVVICIPSGSTEVEIRAVRDSAEHAGGRDVYMLFEPMAAALGIGLDVEIPEGNMIVD
                     IGGGTTEIAVISLGGIVSNKSIKIAGDDLTEDIMDYMGRQHNMKVGERTAEQIKINVG
                     SVLTELQDPPEDFVVHGPNRMTSLPMDVPVSYQEIAHCLEKSISKVDSAVLSALEQTP
                     PELYADIVRNGIYLTGGGALLRGLDKRLTDKIGIPFKVVDDPLHVVAKGTGIALRNID
                     KFTFLMR"
     gene            8076..>8414
                     /locus_tag="PLI30_04750"
     CDS             8076..>8414
                     /locus_tag="PLI30_04750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HOI78898.1"
                     /translation="MKNLLSFIIRNSSWLIAIGLIVISFYLVFSFNSYQRSVYLTSAN
                     KATGWFYEISDKATAFIHLNKSNKLLLERNARLEEELYSLKSYLSDTQRDTLTTNAFL
                     SDSIPASRFSF"
BASE COUNT         2309 a         1780 c         1929 g         2396 t
ORIGIN      
        1 atttttaact gcgaaacatc agaatcttat gtgttaagtt atataagttt ttcaatcgtc
       61 ccttttaacg ttacggataa attcatgaac aaacataata caaattaaca ttggtttttt
      121 aaattatttt atataattca cgcagtaatt attgtaaata acgcagaata tattgttatg
      181 tacggataaa atcgcaaagc gataaaaaat atttaaagct atgtcttggt gtatagtctg
      241 ccaggtatta ggatgttacc agatctaccc ttaacatttc tgtagctaaa tcttcattat
      301 atcttaatta aaaagtaagc ggatcgtaac attcattgcg tagttttgct gtacgaaatt
      361 tacaatttct acaaaatgaa aagaaccatt tcatccgttt tcttcttttg ggttatcact
      421 tcttttttct tcgtagaggc gcaacatgcc cgaattggtg tgatatccga tctgcattac
      481 acgcatccgg cactgattgt tgagaaaggc aaagcgctgg aagattattt acggcgcgac
      541 agaaaacttc ttctcgaaag cgacgccttg ttgcggaaat cggttgagaa tctgttgtgc
      601 gaggatgtaa atgtagtcct tattcccggc gacctgacca aagacggaga attggttagt
      661 caccgtggag tggtggaact cttgtctccg ctcagggaaa aaggagtgaa ggtattggtg
      721 gttcccggaa atcacgatat cgacaacccc gacgccgtaa gctttcacgg aggtgaaacc
      781 cacaaggttg agtcggttac acctgctgaa tttaaagata tttactcgga ttacggctat
      841 gacagcgctc tcagtctgga tgaacattcg ttgagttacg taaatgaacc tgtcgaggga
      901 ttaaggataa tttgtatcga tgcctgtaaa tatcgcgaca acacttttat ctaccggggt
      961 gcgaaaaaag attcgtgtgt cactcacggt accgtaaaac cggaaaccat gaactggata
     1021 aaaaatgaag tccggcttgc taagttgttg ggaaaacaag tgattgccat gatgcatcac
     1081 ggagtagtgg aacatttcga ccatcagtct ttttttgcta gcccttacct cgtcgataat
     1141 ttcgaatggg ttcagcaatc attcatggaa gccggcctga atgttgtgtt taccggacac
     1201 tttcatgcat cggatattgc ccgaatcgaa gatgaaaaag gcaattatct ttacgatatt
     1261 gagacaggct ccgctgtgac ttatctgtgt ccttaccggg tgattgatct ttcgaataac
     1321 catctggaca tacaaacaaa attaacggat agtatagatt ttcctattcc ttcgggtatg
     1381 aatttccagg cttatgcgaa aaagatggtt gatgtaggct ttaatgagat gatatcggct
     1441 cttatcagtg aatatcattc atccttgcct gtatatttac cccgatggct taaggggata
     1501 atccgcattc ccgatgcgga gaagtttacg gatatggtat tgttcaactt gtctccctcg
     1561 gctgtggaaa tgcttgttgc gcattacgga ggaaacgaaa acaatataga gaatgcgcac
     1621 cggcataaag aggagttgtt attagccatt gacagttttg tgcgtgactt gtgtaaagca
     1681 tctgctggaa gcttgtcggg gattatagag aaacttgttg tacgcagcaa gacgattaaa
     1741 aagaccaaac aggccgtctc aagcatttgg gaaaacactt ttcttacggg aaagggagtc
     1801 gtaaagggaa ggctgatgga taactctata aacgacttga attatcttct aacactcaat
     1861 actaatgaaa atataaacaa cggaaacttt tacgctaatg aaaatgtcaa cgatgaggta
     1921 gaagtgaagt tgttttccga ataatatttt tcattctctc tattttaagc ggctctactc
     1981 accgctctac gcagccggca agtgattgta ggctgttttt tatgagcaga gttaactaaa
     2041 aggcacgcca atcacttgga atgccttttc aaaataataa ataaaaagta gagagttttt
     2101 ttatttctgc atttctgctg taatgtcctt aaaactgccg cctgtaaacc tgtacaaccg
     2161 ttgattggtt gtttttttgt ttaaccctcc cagtgcttcc agataggggc ctgttttcac
     2221 ataatcaaaa cgaccagtag ccataggatg caggttactg tttcctgaat accatgccgt
     2281 tttaagcttt cctttccaac ccatccggat atggtttgcc agctggcata cctcttcggc
     2341 atgagcatcg ccccccataa aacagatgca ggtgattgaa tgctcataga gctgcagcag
     2401 tctcgaaagg gctccttcgt tcagttcttc ccctatgtct tgctgcaggt ggggactgtg
     2461 gcaccctttg cagcggtgcg ggcaacccga caggttgacg gctaacgtca cctcgtcggg
     2521 aatctcttga aaaacaatat catagtctgt atatttaagc ataaaaaatt atcttatggt
     2581 tgaaatgtcc gcttttctgt aatacctttc tggcatctca ttctcaaaca tagctttggt
     2641 atgcttgcgg ctaatgtata accttaataa atactaggtt cctctttagg cttctctgtt
     2701 gtgtccggct ttccgtaatg tcttctttcc gcttccgctt gccgggcagc cgagaaattg
     2761 ctcaccctct tcatatagcc gataatgcgt gtgaggtagt ccacattcag ggatctgcat
     2821 ccggggcatt ctttcagaaa gcgcttgtcg atgacgccac aatcgttgca tacggtattt
     2881 ggaatgttga aggtgaaata gttgcagcct tcgtgggctg ctacgcgcaa taactgtcgg
     2941 tattgtgctt ttgagagatg ttcctccagg ttcaggtgaa gcgccgatcc gcccgtcagg
     3001 tgctcaatat agtcccgtcc atgcaggcgg aacttgtcga tcacgttcag cgatggatct
     3061 tccacccggt aaaaatagct attgtagcag tcgcgcggta caaaataacc gtcctccctg
     3121 tcccatttgg catgtttcac gcccacgttt tccgcaggaa tcatctcaca gttgaacatc
     3181 aattctctcg accgatattt tttgttgtat cgctcaatga tcccgagcac ctcctgtacg
     3241 aaggccttat attgaggatt gtggttgatg gctatcccca gcgattcagc cgcctccact
     3301 aacccattta caccgatagt aaggtattga cggtccaagt tgatgtatcc cgcgtcgaac
     3361 agtggcagca tccctttttg ttgaaacatc ttcaggtttt cgttataggc ggtctgtacc
     3421 ttgtgtgtca agtccaccac ctcttcaagg aaagtactgt agggttgtcc cgtacgtttt
     3481 gcgaactgaa cgcagcggtt caggttgatg gtgagcacgc ttttcgatcc ggtggacact
     3541 ccgccggccc ccaatgtata actgaacccg ttgtcctgaa tcttgttgcg caggcgacag
     3601 cagctcgata atgagtcggc attgtcgctc atataggtaa agaaagagtg tccttcggca
     3661 tacatttcgg cggtaaaatc ggcatattct gtatcgatca catcctcttc ccgcgagagc
     3721 aatgccatcg tttccaccgg aaaggtaagc acggttttgg tgcgttcctt gttaaaccat
     3781 ttcataaatc gtttctgtaa ccagctgagc gattcccata cggggcgtga tccgtcggga
     3841 aaaaggaatt ccccgaacag gctttcgaag tagagcctgt cgtagtagga aatattccag
     3901 aaaaccgcct ggaaattacg tgctcccgta ggctggttga tggaataaac gatctgttcg
     3961 aagcagtcgg taatcatttt gtctattgtt cgttgcctcc ttgacaggtc caccacgtcc
     4021 ccggaacgcc cgaaatagtc gtcgccgtac tcttttcgga taaaatagtc caggtacatc
     4081 aggaactcgg gcgtggcgca ggcgccgctg agcatgctcg acacgatgaa caccatattg
     4141 atgaacccgc cgcagaacga tttcaggttg gtcgggggca tggagttgcc accgatggga
     4201 agggtgccgt tcagcaacca cgggtacatc gtgatgctgg cacagtagtt ggctaggctg
     4261 gtctcatcgt ttttatagat aaaatgctcg ttcagcatac cgatgtattt gtcggccagt
     4321 tctttcccat acatctcctt tatccggtcg gtgatcatct ttcggttcag gtcgatgaag
     4381 ttctttttgg gtaattcccc gatgagggtg gcaatgtttt tcttctctac gttggcattg
     4441 gcatcgaact tgcttcccgt tgctgcgttc tgtgcgttgc aatagttcat caggaagttc
     4501 atcttgctct ggatttcacg tgtttcggta tgtttctgcc ggtagagcat gtatgctttt
     4561 gcaacggcga agtatttttc cctcatcagc gctgtttcta cctggttctg aatttcttcc
     4621 acagtcatgc cgtcagaaat gtggaggtgg gagaggagcg tagtaaagtc ctcttcggtg
     4681 gcgaaagatc ctactgccag aaatgctttt gcaacggcat ttctgatttt ctcgatggag
     4741 aagggaacct ttttcccgtc tcgttttaca ataaaaattt ctttgttgcc catatttaga
     4801 catttagata attggataaa tagcccttga aacctgcaaa cctgccagtt tttaaaataa
     4861 caaataggga tatcaaattt ttaaccgtct ttttacccga aagacatcaa aaagagtata
     4921 taaatacatc tggcaggtct tctgacttac tcccgtgtct gaacgccttc ccgttccggt
     4981 ttatcgaaac agtggctatg gaggtgtttg ttcagcacgc aaaaaaagag cttacagcag
     5041 cgggaactgt tgccgatttt cacggcattc ccttttattt tattcataaa gacaactatg
     5101 aataaaaacc aaatgttacc acaaaggtaa atttactttt ttagaaaacc aataaagttt
     5161 tccggaaaag ttatcaacat aggtgctttt attttttata tttctgatta ttaaatttat
     5221 atattttaat ttcgatagtt ccttgattaa attcaagtga caatattttc ttttttttct
     5281 actgattaat tgctatactt tgcataatta tttaaaaaag catgataaaa gcggtcgcca
     5341 tccggtcgca caaccccgaa cagcttcttt ttctcaaaaa ttcataaaac aaactccaat
     5401 ttcacaaaaa tgaccgatag attttatacc tttgtattct ttaaaaaaat aatcattgat
     5461 gtcaaaggaa ataaattcgg cactcatttc ggtataccac aaagacggcc tggaggagat
     5521 attgaaattg ctgaacagtt taaacgtaaa gcttatatca acggggggaa cccgtgattt
     5581 tatccagtcg ttgggttatg agtgtgaatc ggtggaagag gttaccggct atccatctat
     5641 tctaggcgga cgggtaaaga cactgcatcc taaagttttc gggggaattc tgtaccgccg
     5701 tgacgaggag caggaccgga aaatcatcaa atcctatgat attccctcta tagacctggt
     5761 catcgttgat ttgtatccgt ttgaagaaac cgtagctaca caagctcccg aagatgaaat
     5821 tatcgagaaa atagatattg gcggaatctc gcttattcgc ggggctgcca aaaactttaa
     5881 agatgtgatt gttgtggctt ccaggaagca atataaaccg ttgctcgatc tgttaaaaga
     5941 gaaaagcgga aataccgata ttgaagatcg gcgctggttt gccaaggaag cctttgccgt
     6001 ttcatcggga tacgattcgg ctatttttaa ttatttcgac aagggcgaaa gccccgtttt
     6061 gcggatttct gccgacagtg gcaaaacgtt gagatacggg gaaaatccgc atcaaaaggg
     6121 cgttttttac ggtaacttta atgaaatatt tgagcagttg cacggcaaag agatttcgta
     6181 caacaacctg ctcgacatag acgccgccgt ttcgttgatt gccgattttg atgaaacagc
     6241 cttggctatt ctgaagcaca acaatgcttg tggcatggcc tgtcggaata cggtaaaaga
     6301 agcctgggat gcagctctag cggctgatcc tatctctgct ttcggcggaa ttgtggttac
     6361 taaccgtcag gtggatgcag atgccgctaa agtcataaat gaaattttct tcgaagtaat
     6421 tattgctccc gattattcgc aggatgcgct caatatcctg ttccagaaga agaaccggat
     6481 catccttaag caaaaaataa ccggaaagga ggcaaacaat acacaggtcc gttctgcatt
     6541 aaacggattc ctggtacagg ataaagatac gagtattcag acaagaagcg atttaacggt
     6601 ggtaaccgca aaagttccaa cggaaaaaga aacggaagat ctgctgttcg caaatatcct
     6661 ggtgaaacat acgaagtcga acgctatagt gctggcgaaa aacaagcaat tgattgctag
     6721 cgggaccgga caaacatcgc gagtggatgc gttgaatcag gcgatagaga aagcaggaac
     6781 gtttaaattt aaccttaacg gggccgtaat ggcatcggac gctttctttc ctttccccga
     6841 ttgcgttgaa atagcgcata aagccggaat aacagctgta gttcagccgg gaggctccat
     6901 caaggatgca gagtcggttg cttactgtaa cgaaaacggc ctggccatgg ttacaaccgg
     6961 gatcaggcat tttaaacatt gattgagaag ccaggggtgg gttctcgcag tgtgaacaca
     7021 ccctgcaact cgtaacaaac ataacaatat gggattattt tcattcacac aagagttggc
     7081 aatggacctg ggaaccgcca actccattat tgtaaacaat gccggaaaaa ttctgctgga
     7141 cgagccgtcc atagtggcat tggaccgcaa gaccgacagg atgatcgcgc tgggcgagaa
     7201 ggcccgccaa atgcacggaa aaacacacga aaacatacat acggtcaggc ccctgaggga
     7261 tggtgtaatc gctgatttta acgccgcaga acagatgatt cgcggtatga ttaaaatggc
     7321 tacaaaaaac aaaggcggat tattttcgcc gtcacttcgg gtagtaatct gtattccgtc
     7381 aggaagcacc gaagtggaaa tccgtgcggt tcgtgactca gctgaacatg ccggaggacg
     7441 cgatgtttac atgctcttcg agcctatggc agctgctctg ggtatcggcc tggatgtgga
     7501 gattcccgaa gggaacatga ttgtggacat aggtggggga acaacagaga ttgctgtgat
     7561 ctcactgggc gggatagtat cgaacaaatc gataaagatt gccggtgacg acctcaccga
     7621 agacattatg gattatatgg ggcgtcagca caacatgaaa gtgggagaga gaaccgccga
     7681 acagatcaag attaatgtag gctccgtctt gaccgagctt caggatcctc cagaagattt
     7741 tgtcgttcac ggtcctaatc gcatgacgtc tttgccgatg gatgtgcccg tatcctatca
     7801 ggagatagcg cactgccttg aaaagtcgat atctaaagtg gattcagcgg tgcttagtgc
     7861 actggaacaa actcccccgg agttatatgc cgatattgta cgtaatggaa tttacctgac
     7921 aggaggcggt gctttgcttc gtggtttgga taaacggctg accgataaaa tcggtattcc
     7981 gttcaaagta gtagatgatc cgttacacgt ggtagccaaa ggaacaggta ttgctttaag
     8041 aaacattgac aagtttacat tcctgatgag ataggatgaa aaaccttctc tcgtttatta
     8101 tcagaaacag ttcgtggctg atcgcaatcg gtttgattgt catcagcttc tatctggtat
     8161 tttcttttaa ttcttatcag agaagtgtat acctaacatc tgcaaacaag gctactggct
     8221 ggttttacga gatttctgac aaagcaacgg cttttatcca tttaaacaag agcaacaagc
     8281 ttttactgga acggaatgca cggctcgaag aagaactata ctcgttaaag tcctacttgt
     8341 cggacacgca aagggacacg ttaacaacga atgcttttct gagtgattcc attccagctt
     8401 caaggttttc attt
//
LOCUS       DAOSDR010000083         8363 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrimonas sp. isolate AMR_MDS_1826
            NODE_3177_length_8363_cov_4.324386, whole genome shotgun sequence.
ACCESSION   DAOSDR010000083 DAOSDR010000000
VERSION     DAOSDR010000083.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863355
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrimonas sp. (wastewater metagenome)
  ORGANISM  Petrimonas sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Dysgonomonadaceae; Petrimonas.
REFERENCE   1  (bases 1 to 8363)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8363)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:10:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,720
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 35
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8363
                     /organism="Petrimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3177_length_8363_cov_4.324386"
                     /isolate="AMR_MDS_1826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2023866"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..476
                     /locus_tag="PLI30_04755"
     CDS             <1..476
                     /locus_tag="PLI30_04755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002561674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HOI78899.1"
                     /translation="SCEHAAETIAKRHPEVKFLRDATMDMLKEVKNEISEEDYMRAEY
                     VIEETQRVRDVSDALERGDYETVGEKMYETHHGMSKLYEVSCEELDFLNDVAKEHGVT
                     GSRVMGGGFGGCTINLVKDELYDSFIADAKLKFKEKYGHEPKVYDVVISDGARKL"
     gene            601..1293
                     /locus_tag="PLI30_04760"
     CDS             601..1293
                     /locus_tag="PLI30_04760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008779607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HOI78900.1"
                     /translation="MSTGFYSHDAKFLVAVDCIIFGFKNQELNILLTRRPLEPNKGEW
                     SLMGGFMDENESLDKAAEKVLFRYTRQRGIYMEQVGAYGEIERDPGDRVVSVTYYALV
                     RLEEFDTSLAKAFDAKWVNIQQLPRLIFDHNLMVDDALRLLRYKVSLQPIIFEFFEGK
                     FTLPALQDLWEAIYQMPIDKRNFRKKIAAMGILDKLDVKDKSSSKRGAFYYTFNQKKF
                     NEFIESGNRFSL"
     gene            complement(1295..2950)
                     /locus_tag="PLI30_04765"
     CDS             complement(1295..2950)
                     /locus_tag="PLI30_04765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006799603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HOI78901.1"
                     /translation="MNQNSFLGLIEQSIRNHWDLPAMSDFQGKTLYFKDFAREIDMLH
                     EYFNSAGIQRGDKIAICGKNSSHWAISFFATLSYGAVAVSILHEFEKESVQYIVDHSD
                     SKMFFVDEAIWREIDETKIPKAETVLSLDDFSLLKVTSKEFKIFVSNSFSFFDKKYEK
                     GFFVNDIAFRTDKPEEVAVINYTSGTTSSPKGVMIPYRSLWSNTRFANDSLDFIHSGD
                     NMICMLPMAHTYGLAFEVLNAISMGFHIHFLGKTPSPKVLLDAFARIKPKLVLAVPLI
                     IEKIVIKNVFPKLQQGAAKTLIKIPLLNAVVYHKIRKSLIDVFGGNLVEVVIGGAALN
                     ADVEKFLRKIKFPYTVGYGMTECGPLISYSFWTEFKERSCGKVVDRMQVKIDSDDPQN
                     VVGEILTKGTNLMLGYYKNEEATKAVFTEDGWLRTGDLGILDKDNVVFIRGRNKNMIL
                     GPSGQNIYPEEIEDKLNNSPYILESLAIEEKGKIIALIVPDTEVLKAENISPEQYNPV
                     FDKEINAINAKLANYSKIASFRLQAEEFEKTPKRSIRRFKYQK"
     gene            3301..5541
                     /locus_tag="PLI30_04770"
     CDS             3301..5541
                     /locus_tag="PLI30_04770"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009318889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH92 family glycosyl hydrolase"
                     /protein_id="HOI78902.1"
                     /translation="MRQSYRKTYVIGCFIIFLITSCTSSQSTDLVDFVNPYLGNISHL
                     LVPTYPTVHLPNSMLRVYPERADYTGDLLNGLPVAVTSHRGSSAFNLSVFQGDELELK
                     PVIPYSYDREKITPYDYFVYLDEQETSVYYAVSHQSGIYELTFGHTDPVYLILNSRRG
                     ELQWDGKAFSGYQQLQNNTRIFVYMEPDIQPQGANDKAASGQNAHLVLSYPSNTKQLK
                     LRYGISFIDTVQAKKNLYREMSGFDPSGVAEKGRQTWNETLGKIKVDGGSYDDKVVFY
                     TSLYRTYERPVCISEDGRYFSAFDGEIHNDNGAPFYTDDWIWDTYRATHPLRTIIETE
                     MESDILNSFLKMSEQMPEFWWPTFPEITGDSRRMNSNHGIATVIDAYRKGLKGIDLAR
                     IYPAVRNAVMEKTLAPWSGKPSGELDRFYHEKGYVPALREGEEETIPEVHGFECRQPV
                     AVTLGTSYDEWCLAQIARELGKEDDYEYFLKKSYNYRHLFNPQTGFFHPKDKDGNFIE
                     PFDYRFSGGVGARQYYGENNAWVYRWDVQHNVADLIHLMGGRENFIRNLDQTFREPLG
                     KSKYDFYSQLPDHTGNVGQFSMANEPSLHIPYLYNYAGQPWKTQKRIHILLNQWFRND
                     LMGVPGDEDGGGMSAFVVFSQMGFYPVTPGIPAYNIGSPFFAKTVISLPGGKAFTVIA
                     RNFSEENKYIQSAELNGIPWEKPWFSHDDLKNGGTLELIMGNKANKQWGSASQDAPPS
                     EGELTD"
     gene            5710..6876
                     /locus_tag="PLI30_04775"
     CDS             5710..6876
                     /locus_tag="PLI30_04775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008154974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOI78903.1"
                     /translation="MIQKKLKMGMVGGGSDAFIGAIHRNAAFMDNLIELVCGCFSVNP
                     EISRSSGREYFLPDNRIYTTYQEMFEKEMLLPEEERMDFVTIVTPNKWHFEPAMMALE
                     RGFHVVIDKPMTFSLEEAKILQKKVEETGLVLALTHVYSGYPAVKEAKARIAEGKIGK
                     LRRVYVEYTQGWLSKRIEILGGNNAGWRTDPARTGKAGTMGDVGTHAWHLAEYITGLK
                     VEEVCADLRTFVEGRPVDDDGAALLRFENGVAGVLMATQIATGEANNIRIRVYGEKGG
                     MEWRQMDPNRLILRWEDRPTEELYMGSNEYMSDLALWNTRIPSGHPEGFIEAFANIYR
                     NFVLTVLAKKRGGAPDYKILDFPTVADGVRGMQFIETIIASGNDHCNKWCKWIE"
     gene            7025..>8363
                     /locus_tag="PLI30_04780"
     CDS             7025..>8363
                     /locus_tag="PLI30_04780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005646120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HOI78904.1"
                     /translation="MAKSKNIYGDQHKKNRKKFHQRKGFILLLGIAIGIIFIGALYQT
                     SVYFSTNESCMMCHVHPHAEESWKLSVHVNNGSGVMVNCVDCHLPPKDDTWAHYTAKA
                     ALGIRDVWGYLTKDSADFDWDRKSELEHAIKYIPNASCVKCHQNLFPQGITDDGITAH
                     MYYDENHEKLNLQCISCHLDAGHYNPNYSHGQMVGIPGMNVATAVDSSKFFKEAAAVT
                     AFENYTEQIPGTAVSFNMKAVPGGTYTMGSSPKEPFHKTDEAPQHEVKVSPFFMAEVE
                     TTWDQYWAFYANTMSEGRTPPEVVYENNLKAMGVDAISGPTPPFGYPDQGWGSGDRPA
                     ITMTHYAAETFCQWLSQKTGKKYRLPTEAEWEYAARGGTQTPYFFTGSPKDFSDEGFM
                     RKFFPAKTDSISAFVIYGKNSDNRTQEPSLVKPNPFGLKNMLGNVMEYTADKYD"
BASE COUNT         2433 a         1752 c         1926 g         2252 t
ORIGIN      
        1 aatcgtgtga gcacgctgcg gaaactattg ctaaacgtca tcccgaagtg aagttcctac
       61 gtgatgcaac aatggatatg cttaaagaag tcaaaaacga aatctcggaa gaagattata
      121 tgcgcgccga gtacgtgatt gaagagactc aacgggttag agatgtgagc gatgccctgg
      181 aacgtggaga ttacgaaacc gttggggaga aaatgtatga gacgcatcac ggaatgagca
      241 aactttatga agtgagctgc gaagaactcg atttcctcaa tgatgtggca aaagaacacg
      301 gagttacagg ttctcgggtt atgggcggtg gctttggtgg ttgtaccatc aatcttgtta
      361 aagacgaatt gtatgattcg ttcatagctg acgctaaatt aaagttcaaa gaaaaatacg
      421 gacatgaacc taaggtatac gacgttgtca taagtgacgg tgcacgtaaa ctctgaagta
      481 gtttaataat atacttcgcc ggtaatagcg cttcgcgggt gatattccgg acgtcaaact
      541 tttatcggcg aagttgtttt ttcttgcctg ttgatccata acttctaaaa ttctaatatt
      601 atgtccaccg gattttattc tcacgatgcg aaattccttg ttgcagtaga ttgcattatt
      661 ttcggattca aaaaccagga acttaatatc ttgttaactc gtcgcccgct tgaacccaac
      721 aaaggagaat ggtcgcttat ggggggattt atggacgaaa atgagagtct tgacaaagct
      781 gcagaaaaag tactgttccg gtacacccgg cagagaggta tttatatgga acaggttggg
      841 gcctatggtg aaattgaacg tgatccgggc gacagggtcg tgtccgtaac ctattatgca
      901 ttggtacggt tggaggagtt tgacacgagc ctggccaaag cattcgacgc caaatgggtg
      961 aacattcagc aacttccccg gctgattttc gatcacaacc ttatggtgga tgatgcattg
     1021 aggttattga ggtataaagt ctccctgcaa cccatcattt ttgaattttt tgaaggaaaa
     1081 ttcacgcttc ccgctttgca ggatttatgg gaagccatct atcagatgcc aattgacaag
     1141 cgtaatttcc ggaagaaaat cgcagcgatg ggcattctcg ataaactgga tgtaaaagat
     1201 aagtcttcct ccaaaagagg agcgttttac tataccttca atcaaaagaa attcaacgag
     1261 tttattgagt ctggaaacag gttttccctg taatttattt ctggtactta aaccttctaa
     1321 tgctccgctt aggcgttttt tcaaactcct cggcctgaag cctgaatgaa gctattttgc
     1381 tgtaattggc tagttttgcg tttattgcgt ttatttcctt atcgaagaca gggttatact
     1441 gttctggtga tatgttttca gctttcagca cctcagtgtc gggaacgatg agtgcaataa
     1501 ttttgccctt ttcttctatt gccagtgact ccaatatata gggagagttg tttagcttat
     1561 cttcaatctc ttcgggatag atgttttgcc cggaagggcc gagaatcata tttttgttcc
     1621 tgcctctgat aaatacaacg ttgtctttat ccaatattcc aagatcgcct gtcctgagcc
     1681 acccatcttc cgtaaaaaca gcttttgtag cttcttcgtt tttataataa ccgagcatca
     1741 ggtttgtacc tttggtcaag atttccccaa caacgttttg cggatcatcc gaatcaattt
     1801 taacctgcat tctgtccact acctttccac acgatcgttc tttgaattct gtccagaacg
     1861 agtaagaaat aagcggccca cattcggtca tcccgtaccc aaccgtgtag ggaaatttta
     1921 tttttcgcag aaatttttct acgtctgcgt tcaaagctgc tcctccgatg acgacttcta
     1981 ccagatttcc tccgaaaacg tcaataagcg atttccttat tttgtggtaa acaacagcat
     2041 tcagcagtgg aattttaatc aatgttttgg ctgctccctg ctgaagtttc ggaaagacat
     2101 ttttaataac gattttttcg ataatcagag ggacggctaa taccagcttg ggttttattc
     2161 ttgcaaaagc atcaagcagt accttgggag aaggcgtttt tcctaaaaaa tggatgtgga
     2221 aacccataga aatggcgttg agcacctcga aagcaagtcc gtaagtgtga gccatgggta
     2281 acatgcaaat catgttgtct cccgagtgga taaaatcaag gctgtcgttt gcaaaccggg
     2341 tgttcgacca taaactccgg taagggatca tcactccttt cgggctgctg gtggttcccg
     2401 atgtatagtt tataacagcc acttcttccg gtttatcggt cctgaaagca atgtcattga
     2461 caaagaatcc cttctcgtat ttcttgtcga aaaaagaaaa cgagttggac acaaatatct
     2521 tgaactcttt cgatgtcact ttcaacaagg aaaaatcatc gagcgagagg acagtttctg
     2581 ccttcggtat cttggtctca tctatttctc tccaaatggc ttcatcaacg aaaaacatct
     2641 tggaatccga atgatccaca atgtactgaa cactttcttt ctcaaactca tgcaaaatac
     2701 tcactgctac agctccgtat gagagcgtag cgaaaaaaga gatggcccaa tgggacgaat
     2761 ttttaccaca tatcgcaatt ttatcgccgc gttgaatgcc ggcagagttg aaatattcgt
     2821 gcagcatatc aatttcacgg gcaaaatctt tgaaataaag tgttttgccc tgaaagtcgc
     2881 tcattgcagg taaatcccaa tgatttcgta tactttgctc gattaatcca agaaaagagt
     2941 tctgattcat tattgtatgt ttttgttatt atacaaacaa ttaagtttga ggattgtttg
     3001 ttcggattta atataaataa atgtcaaatt cttgcttata ctgataaaat aattaaattt
     3061 gtgcggacgt aaagtcaaca tgttacaaat ttagtaaact ttgtcataaa tgaaaaacag
     3121 cctcactgtt tttgtttttt gcttcacggt gatttctctc tgggaacgaa gtgctactac
     3181 tcttgttgaa accccttgtt caccctgacg gaaataaata gaaattctaa atttaaaaca
     3241 gttcctaaat gtacaataac tattaaaagg atgagaaaga tgctatttct gtacagatta
     3301 atgaggcaaa gctaccgcaa aacatatgta atcggttgct ttattatctt tttgattact
     3361 tcatgtacaa gttctcaatc aactgatctt gttgattttg ttaaccctta cctggggaac
     3421 atcagccacc tgttggtgcc gacgtacccc acggttcatt tgccgaacag catgttgcgt
     3481 gtttatccgg aacgcgcgga ttacacgggt gatttgctga acggcttgcc cgttgcggtg
     3541 accagccacc ggggaagttc ggcatttaac ctgagcgtct tccaagggga cgagctggaa
     3601 ttgaagccgg ttattcccta tagctacgac cgtgaaaaaa tcacacccta cgattatttt
     3661 gtctatctcg acgagcagga gaccagcgta tattacgccg tttcccacca atcggggatc
     3721 tatgagctga cattcggaca taccgaccct gtatacctga tcctgaactc ccggcgtggt
     3781 gaacttcaat gggacgggaa agcattttcg ggttatcaac agctgcaaaa caacacgcgg
     3841 atatttgtat atatggaacc ggacatacag ccccagggag caaatgacaa agctgcttcc
     3901 ggccaaaatg cccaccttgt tttgtcgtac ccctcaaaca caaagcagtt gaaacttcgt
     3961 tacgggattt cgtttatcga tacagttcag gcaaagaaga acctctaccg ggaaatgtcc
     4021 gggtttgacc cctcgggcgt tgccgaaaaa ggacgtcaaa cctggaatga aaccctcggt
     4081 aaaattaaag tggatggtgg ttcgtatgac gataaagttg ttttctacac ttcgttgtac
     4141 cgaacctacg aacgtccggt atgcatatcg gaagacggac gctacttcag cgcctttgat
     4201 ggggaaatcc acaacgataa cggggcgcct ttctatacgg atgactggat ctgggatacc
     4261 tatcgggcaa cgcatccgct tcgtacgatt atcgaaacgg aaatggagtc ggatattctc
     4321 aattctttcc tgaagatgtc cgaacaaatg cccgaattct ggtggcccac cttccccgag
     4381 attacgggcg acagccgccg gatgaactcc aaccacggga tagccaccgt tatagacgct
     4441 taccgcaaag gattgaaggg cattgacctg gcaaggatct acccggccgt tcgcaatgcc
     4501 gttatggaga agacgctggc tccttggtcc ggcaaaccct cgggtgagtt ggatcggttt
     4561 tatcacgaga aaggatatgt tcctgctctt agggaagggg aggaagaaac cattcccgaa
     4621 gttcacggtt ttgaatgtcg ccagccggtg gctgtaacgc tgggcacctc ctacgatgag
     4681 tggtgcctgg cacaaattgc ccgggagttg gggaaggagg atgattatga atacttcctg
     4741 aagaaatcct acaattatcg ccatcttttc aatccccaga ccggcttttt tcaccctaag
     4801 gacaaggatg gaaacttcat cgaacccttc gattatcgtt tttcaggagg ggtgggtgca
     4861 cgacagtatt acggtgagaa caacgcatgg gtataccgtt gggatgtgca gcacaacgta
     4921 gccgacctga tccacttaat gggggggcgg gaaaacttta tacgcaacct cgatcaaaca
     4981 tttcgcgaac ctcttgggaa aagcaaatac gacttttaca gtcagttgcc cgaccatacc
     5041 ggcaatgtgg gtcaattctc gatggctaac gagccgtcgt tgcatattcc ttatttatac
     5101 aattatgcag gacagccctg gaagacgcaa aagcggattc acatactcct gaatcaatgg
     5161 ttcagaaacg acctgatggg agttcccgga gatgaagacg gaggcggaat gtcggctttt
     5221 gtggtgtttt cgcagatggg gttttatccg gtaacgcccg gaattccggc atacaatatc
     5281 gggagtccgt tctttgcaaa gacggtcatt agccttcctg ggggtaaagc cttcaccgtt
     5341 atagcccgta atttttccga agagaacaaa tacatccaat cggcggaact caacggaata
     5401 ccctgggaaa aaccctggtt cagccacgac gacctcaaaa acggcggaac cctggaactt
     5461 atcatgggca ataaagcgaa caaacaatgg ggtagtgctt cgcaagacgc gcctccttcg
     5521 gaaggagaat taacagacta aaaaacagta aaaatgaaag aagaattggt ttttctttca
     5581 acgataggcc aaaatcaaaa gatgttctaa ttgtaaaaca ttcggtaaaa ttaataataa
     5641 cactgaaaat tgaagaataa aacggatatt tacatttttt aaaaatatat agttttaatg
     5701 cacggtatca tgattcaaaa aaaacttaaa atgggtatgg ttggtggcgg aagtgatgct
     5761 tttatagggg caattcatcg taacgctgct tttatggaca atctgattga attggtatgc
     5821 ggttgtttca gcgtaaatcc ggaaatatcc cggagttccg gaagggaata ttttctcccc
     5881 gacaacagaa tatacactac ctatcaggag atgttcgaaa aagaaatgct tctgcccgaa
     5941 gaagaacgga tggattttgt gaccatcgta acccccaaca aatggcattt tgaaccggcc
     6001 atgatggcct tggagagagg ttttcacgta gttattgaca aacccatgac cttttcgttg
     6061 gaagaagcaa aaatactcca aaagaaagtg gaagaaacgg gccttgttct ggctcttaca
     6121 cacgtctatt caggttatcc ggcggttaag gaagccaaag ccaggattgc tgaaggtaaa
     6181 ataggaaaac tgaggcgggt ttacgtagaa tatacacaag gctggttgtc gaaaagaatt
     6241 gaaatattgg gcggaaacaa tgcgggatgg cgtacagatc cagcccgtac cggtaaagca
     6301 ggtaccatgg gagatgtggg tacccacgcc tggcatctgg cggaatacat caccgggttg
     6361 aaagtggagg aggtatgtgc cgatctgcga acctttgtgg aaggacgccc ggtagatgat
     6421 gacggagctg cccttttacg ctttgagaac ggagttgccg gagtacttat ggcaacgcaa
     6481 attgcaacag gtgaagcaaa caatatccga atcagggttt acggtgagaa ggggggcatg
     6541 gaatggcgtc agatggatcc gaaccggttg atcctgagat gggaagacag gccgacagaa
     6601 gagctttaca tgggcagcaa cgaatacatg agcgatttgg ctttgtggaa cacaagaatt
     6661 ccttccggac atcccgaagg gtttatagaa gcctttgcca acatatacag aaacttcgtg
     6721 ctgacggtgt tggcaaaaaa aagaggaggg gctcccgatt ataagatcct ggattttcct
     6781 actgtagccg atggcgttcg cggcatgcag tttatagaaa caataattgc ttctggtaac
     6841 gatcactgca acaaatggtg taaatggatt gaataatatt tatctgcgat ttaaattgct
     6901 tttttgtaca aattaaacct gcaaaccatt aaattttcat atatttgcaa gcttaagata
     6961 tcattttatt ttttacgcgt taaaaagcaa caattaaata tttaattcat taattaatag
     7021 atagatggct aaatctaaaa acatctatgg tgatcaacac aaaaaaaacc ggaaaaaatt
     7081 tcatcaacgt aaagggttca ttttgctgtt gggtattgcg ataggcataa ttttcatcgg
     7141 agcattgtat caaacatccg tttatttttc taccaacgaa tcatgcatga tgtgtcatgt
     7201 tcatcctcac gcagaagaaa gttggaaact ttctgttcac gtgaacaacg gaagtggcgt
     7261 aatggtcaac tgcgtggatt gtcatttgcc gccaaaagac gacacatggg ctcattacac
     7321 ggctaaagcc gctttgggta tcagagacgt atggggatat cttaccaagg acagtgcaga
     7381 cttcgactgg gatagaaaat ctgaactcga acatgctata aaatatattc caaatgcttc
     7441 ctgcgtaaaa tgtcatcaga atttattccc tcagggaata accgatgatg gcattaccgc
     7501 gcatatgtat tacgacgaga atcatgaaaa gctcaatctg cagtgtatca gctgccacct
     7561 cgatgcaggg cattataacc cgaactattc gcacggacaa atggtgggaa tacctgggat
     7621 gaatgtagct actgctgtgg attcaagcaa gttttttaaa gaagctgccg cagtaacggc
     7681 ttttgaaaat tataccgaac agatccccgg tacggcagtc tcgttcaaca tgaaagcggt
     7741 acccggagga acttacacta tgggtagttc acccaaggaa cctttccaca aaacggatga
     7801 agccccacaa catgaagtga aggtaagtcc cttcttcatg gctgaagtag aaactacctg
     7861 ggatcagtac tgggcttttt atgccaatac catgagcgaa ggaagaactc ctcccgaagt
     7921 ggtttacgaa aacaacttga aagctatggg tgtagatgca atctccggtc caacacctcc
     7981 gtttggatat cctgaccagg gatggggctc gggcgacagg ccggccatta caatgacgca
     8041 ttatgccgca gaaacattct gtcagtggct gtcgcagaaa acaggcaaaa agtaccgctt
     8101 acctaccgaa gccgaatggg aatatgcggc acgcggtggt acgcaaacac cctatttctt
     8161 tacaggaagc cccaaagatt tttccgatga agggtttatg cggaagttct tccctgcaaa
     8221 aacagacagt ataagcgcat tcgttatcta cggtaaaaac agcgataacc gtacgcagga
     8281 accgtcgttg gtgaagccta atccattcgg attaaaaaat atgctgggta acgtaatgga
     8341 atacaccgct gacaagtatg atg
//
LOCUS       DAOSDR010000084         8339 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrimonas sp. isolate AMR_MDS_1826
            NODE_3186_length_8339_cov_4.899324, whole genome shotgun sequence.
ACCESSION   DAOSDR010000084 DAOSDR010000000
VERSION     DAOSDR010000084.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863355
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrimonas sp. (wastewater metagenome)
  ORGANISM  Petrimonas sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Dysgonomonadaceae; Petrimonas.
REFERENCE   1  (bases 1 to 8339)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8339)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:10:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,720
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 35
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8339
                     /organism="Petrimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3186_length_8339_cov_4.899324"
                     /isolate="AMR_MDS_1826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2023866"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(251..1240)
                     /gene="trpS"
                     /locus_tag="PLI30_04785"
     CDS             complement(251..1240)
                     /gene="trpS"
                     /locus_tag="PLI30_04785"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006843864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HOI78905.1"
                     /translation="MDTVLSGIRSTGNLHLGNYFGALRNFIRMQDENRCFFFIADIHS
                     LTTHPDPKDLHGNVKNVLVDYLAAGIDPGKSVIYIQSDVPETIELYLYLNMHAYMGEL
                     AKTTSFKEKARKQPENVNAGLLTYPTLMAADILIHNADKVPVGKDQEQHLEMTRKFAR
                     RFNNFYGVEFFKEPVAYNFGEELVKIPGLDGSGKMGKSEGNAIYLADSPKEIEKKVKR
                     ALTDSGPTQPNSVKPDYIENLFTIMRVVSTPEVVEHYENKWNACDIRYGDLKKQLAED
                     IIKVTSPIRERILEIEKDDAYLRKVTREGAEKARESASKTIAAVREIVGFKKF"
     gene            1402..3306
                     /locus_tag="PLI30_04790"
     CDS             1402..3306
                     /locus_tag="PLI30_04790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019538422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HOI78906.1"
                     /translation="MKKFILLFTLSVFGLLTFGQQVERGISIIPEPVSVVKKGGYYVL
                     PDNIVISAPSGDGMNFINEHLAEKLSTAPGKKVTVRNNVASADIVLTVNAKTDAKIGN
                     EGYTLSVTSGKVRINANKPAGLLYGVQTFLQLLPPQVESDKPERNVSWQVPQVEITDY
                     PRVGWRGLMLDVSRHFFTVDEVKQYLDNMVKYKYNLFHWHLTDDEGWRIEIKSLPKLT
                     EVGAWRQEQIGWFGGFSQPDPDAPKNYGGFYTQEQIREIVQYAKERNIQVMPEIDVPG
                     HSSAILAAYPELSCFPESGAHAVRTGAPFMDWNTGGRPAAIYENTLCPSNEKVYDFLD
                     KLMTEVASLFPFEYIHTGGDEAPYTFWEKSPDVKKLMQREGIKDMAGVQSYFGKRLER
                     IILSKGKKMMGWDEILEGGITPTTALMSWRGVNYGIEASKSGHYVVMSPTNYVYIDYM
                     QGDISTEPRVYASLRLNQTYKFDPIPEGADANYILGGQANLWTEQVYNIRQAEYMTWP
                     RGFAVSESLWSPKEKKDWDQFVLKTENHFVRFDYAKTKYSPAIYDPIVRVTRDSEQYF
                     VELTTEISGLDIYTSFDSSTPDNFYPRYAKPQLIPKDAVMMRIITYRGDTPIGRLLSI
                     PVEDLKKRVR"
     gene            3431..4009
                     /locus_tag="PLI30_04795"
     CDS             3431..4009
                     /locus_tag="PLI30_04795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HOI78907.1"
                     /translation="MKTYELKGEVREDFGKKAARSYRSEGLIPCVVYGGSNEKNLNFV
                     VKKSDVRNLIYTPEVYLINLVLDGKSVMAILKEIQFHPVKDNILHIDFLHVFPNVPLV
                     IELPVRLEGLAAGVKSGGKLSLDLRKLKVKALAEKLPQELVVNVEDLELGKSIQVGEL
                     NFEGLELLTPKNAVVCRVQLTRAARGAAAKAQ"
     gene            4030..4593
                     /gene="pth"
                     /locus_tag="PLI30_04800"
     CDS             4030..4593
                     /gene="pth"
                     /locus_tag="PLI30_04800"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005841628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HOI78908.1"
                     /translation="MKYLIVGLGNIGPDYENTRHNIGFRVLDAFAKASNIVFQDKRYG
                     FVAETRLKNKSLLLLKPSTFMNLSGNAVRYWLQKEKIEDRNLLVVVDDLALPFGTLRL
                     KSKGSDAGHNGLKHIEQVLGTQQYARLRFGIGNDFPRGAQVDYVLDEFSGEEKQFLPE
                     RLEKAAEIIRSFCLAGVNITMNQYNNK"
     gene            4614..5018
                     /locus_tag="PLI30_04805"
     CDS             4614..5018
                     /locus_tag="PLI30_04805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013815412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HOI78909.1"
                     /translation="MNEVRIDKWMWAVRIFKTRTVALDACKKGRVLIDNVSVKPSRMI
                     RVGDVVQVRKPPVTFSFKVLGLSDKRMGAKLVPQFMENVTPPEQYEILELNKISGFVD
                     RQRGTGRPTKKERRDLDQFTDKFDFDEFDWDE"
     gene            complement(5022..5738)
                     /locus_tag="PLI30_04810"
     CDS             complement(5022..5738)
                     /locus_tag="PLI30_04810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010802463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-phosphosulfolactate phosphatase"
                     /protein_id="HOI78910.1"
                     /translation="MIVDICLSPALYPYYQKENDVVVVVDIFRATTTMCAAFNNGAAS
                     IIPVADIERAKRYKSEGFLVGAERKTRRVEFADFGNSPFEYTPDKVSGRDIVFTTTNG
                     TRAIEAAKDCGRLFIGAFSNIDNLADTCLSSGERIVVLCAGWNNCVSMEDTLFGGAFV
                     KKLSERAKIVFGSDAVRIAIELWEKARNSPMDYLKNADHYHRLIANGAEGDAAYCLQR
                     NTVSVVPYYNRESKKLTVLQ"
     gene            5808..6404
                     /locus_tag="PLI30_04815"
     CDS             5808..6404
                     /locus_tag="PLI30_04815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006046310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Maf-like protein"
                     /protein_id="HOI78911.1"
                     /translation="MAIANYHLILASNSPRRKELLTGIDIEYEIRTLPDIEETYTNDT
                     PPEKVAEYLALKKTTAYLPQLKDNELLITADTVVLLNNKVYGKPMDNAEAKRMLRELS
                     GQTHRVITGVCLTSTKKQVAFSDTTRVTFTELSEEEIEYYVERYSPLDKAGAYGVQEW
                     IGYVAVKHIEGSYFNVMGLPIHRVYEELKSSFGFRVAG"
     gene            6488..7870
                     /locus_tag="PLI30_04820"
     CDS             6488..7870
                     /locus_tag="PLI30_04820"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019538427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HOI78912.1"
                     /translation="MLTVEKIGGTSMSQFQDVLQNIIKGHRKGDEYYNRIFVVSAYNN
                     VTNWLLEHKKTGEPGIYNKFLNGEDYSSALDSFCQRLLLINDGFADIGLDLKEARDFI
                     RFRVDQAKTLLYSLGKVLASGYVNMTDIHLAAREILASLGEAHSGWNSVNILNNNGIN
                     ARFIDLCGFNDNEPLTIDQRIHREFAGVDFSNIVCVATGYTKGTEGIMRAFDRGYSEV
                     TFSKIAVEVKADEAVIHKEYHLSSADPKIVGVENSVPVGQTNYIIADQLADIGMEAIH
                     PKAAKPLELAGINIRIKNTFEPEHPGTLISRDYISPEARVEIVSGSRKVIALEIHDPM
                     MVGEVGYDARIMQYFQKYNVSYILKSTNANSITMVVWDEERAHELIRVLKEVFYQVTV
                     LPAAIVCAMGTNIAMPGFLYRAAKALHEKGINVESFAQSLMQVNMQFVISREQYEEGV
                     IALNEALCKR"
     gene            7880..>8339
                     /locus_tag="PLI30_04825"
     CDS             7880..>8339
                     /locus_tag="PLI30_04825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019540510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HOI78913.1"
                     /translation="MKAYLSRRKERFFFFFLYSMSLFFFFSCLNPQEKKESENGLLSE
                     EAFKTPDREYYPETWYHFIGGNVSKPGITADLEAIAKAGISGIQLFHGQFGGEWPGVS
                     PQIQTLSEDWDELVQWTAEECKRLNLRFTMQNCPGWSYAGGPWIEPENSMR"
BASE COUNT         2288 a         1785 c         2058 g         2208 t
ORIGIN      
        1 tccgttgcat attgggtgtc gaattttttc ccccagctgt acagattggt gaaacagagg
       61 tccgatgccc tgtaattttg tttgcagagg tgcgaattga taatgtcttt gtcttttaat
      121 tctatggatt taaacggtag cataaaatcg ttgcattgta gttattgagt gtgaacaatc
      181 ttttatgcga aaagtttaac aaaaaaagga atgacactgt cattccgtca aaaatcaggg
      241 gcctgttggt ttaaaatttt ttgaacccta caatctcccg gactgcggca atggtttttg
      301 atgcgctttc gcgtgccttt tctgcccctt cccgggtgac ctttcgcaaa taggcgtcgt
      361 ctttttcaat ttccaggatt cgttcgcgaa tgggtgaagt cactttaata atgtcttcgg
      421 ccagttgttt tttcaggtcg ccgtaacgaa tgtcacatgc gttccatttg ttttcgtaat
      481 gttctacaac ttcgggggtc gaaaccacac gcataatggt aaacaggttc tctatgtagt
      541 ctggtttcac ggaattcggc tgagtgggtc cggaatcagt gagggcacgt tttactttct
      601 tttcgatctc tttgggcgaa tctgccaggt aaatggcgtt tccttccgat tttcccatct
      661 tcccgcttcc gtccagcccg ggaattttca ccagttcctc tccaaagttg tatgcgacag
      721 gctctttaaa aaattccaca ccgtagaaat tgttgaaccg gcgggcaaat ttacgggtca
      781 tctccagatg ctgttcctga tcttttccca cgggcacctt gtcggcgttg tgaataagga
      841 tatcggcagc catcagcgtg gggtaggtaa gtaatcccgc atttacgttc tcgggttgtt
      901 ttcttgcttt ctccttgaat gatgtggttt tggcaagttc acccatgtat gcgtgcatgt
      961 tcagatagag atacaactcg atcgtttccg gcacgtcact ttgtatatag atgaccgatt
     1021 taccgggatc aatgcccgcg gccaggtaat ccaccaatac atttttaacg ttcccgtgta
     1081 aatctttcgg gtcggggtgt gtggtgagtg agtgaatatc ggcaataaaa aagaaacacc
     1141 ggttctcgtc ctgcatccgg atgaagttgc gcaacgcacc gaagtaattt cctaaatgca
     1201 agtttcccgt cgaccggatt ccactgagta ctgtatccat attgaaattg actgttattt
     1261 gaagtgcaaa gataataaat atttacgatt taggatttca tgtgttctgt tgtttgatgg
     1321 aacagaatca cgaaattcat cctttttttc gtaaatttgc aaatcacaaa tcaatcaaac
     1381 aacaaattca atagttttac gatgaagaaa tttattctat tatttacctt atccgttttt
     1441 ggattgctga cttttggcca gcaggttgaa cggggaattt ccattattcc cgaacctgtt
     1501 tccgtagtta aaaaaggagg gtattatgtg ctacccgaca atatcgtgat ttccgctcct
     1561 tcaggagacg ggatgaactt tataaacgaa cacctggcgg aaaaactttc cacggctccg
     1621 ggtaaaaaag tgaccgtgcg caataacgtg gccagcgcgg acatcgtttt aacggtcaat
     1681 gcgaagacag acgctaaaat tggcaacgaa gggtatactt tgtcggttac ttcgggtaaa
     1741 gtgaggatta acgccaataa gccggccggg ttgctgtacg gtgttcaaac gttcttgcag
     1801 cttttgccgc ctcaagttga aagtgataaa cccgaaagga atgtttcctg gcaggttcct
     1861 caagtggaaa ttaccgatta tccccgggta ggctggcgtg ggttgatgct ggatgtttcc
     1921 cgtcattttt ttaccgttga cgaggttaaa caatacctgg ataatatggt gaaatataaa
     1981 tacaacctgt tccattggca cctcactgac gacgaagggt ggcggatcga gatcaaaagc
     2041 ctgcccaaac ttactgaagt tggggcctgg cgtcaggaac agatcgggtg gtttggcggc
     2101 ttttctcaac ccgatcccga tgccccaaaa aattacggtg gcttttatac gcaagagcag
     2161 ataagggaaa ttgttcaata cgccaaagaa cgcaatattc aggtgatgcc tgaaatcgat
     2221 gtccccggac acagttcggc catccttgca gcttatccgg agctgtcttg tttccctgaa
     2281 agcggagcac atgccgtgcg tacgggtgca cctttcatgg actggaatac gggtggccgg
     2341 ccggcagcca tatacgaaaa tacgctttgt ccatccaacg aaaaggtgta tgattttctg
     2401 gacaaactga tgactgaagt ggcttcgctt ttcccctttg aatatattca tacgggtggt
     2461 gatgaagcac cgtacacgtt ttgggaaaag agccccgatg tgaaaaagct tatgcagcgc
     2521 gaagggatta aggatatggc cggggtgcaa tcctattttg gtaaacggtt ggagcggatt
     2581 atcttatcaa aaggcaaaaa aatgatggga tgggacgaaa ttctggaagg cggtattacg
     2641 ccaaccactg cgttgatgag ctggcgcggc gttaattacg gaattgaagc gtccaaatcg
     2701 gggcattatg tagtgatgag cccaaccaat tacgtgtata tcgattatat gcagggcgat
     2761 atcagcacgg agccccgtgt ttatgcttcg ttgcggttga accaaaccta taaattcgac
     2821 ccgattcccg aaggagctga tgctaactat attttaggag gccaggccaa tctgtggaca
     2881 gaacaggttt acaacatccg tcaggcagaa tacatgacct ggccgcgtgg atttgctgtt
     2941 tctgaatcgc tttggtctcc taaagagaaa aaagactggg accagtttgt gttgaaaaca
     3001 gaaaatcatt tcgtgcgttt cgactacgca aaaacgaaat attcaccagc catatacgat
     3061 ccgattgtgc gtgttacgcg tgacagcgag cagtatttcg tggagctaac tacagaaata
     3121 agcggattgg atatttacac cagtttcgac agttcaacac cggataactt ctatcccaga
     3181 tatgccaaac cgcagcttat ccccaaagat gctgttatga tgcgtataat cacgtaccgc
     3241 ggcgatacgc ctattgggcg gttgttaagc attccggtag aagatttgaa gaaaagagtc
     3301 cggtgaaagt ttggataata aaaagattat cactattttt gcgccgcttt ttggaagctc
     3361 cctcgtgtga tgggaaataa agaagcaaaa aacaaacttt attttgagta acacaaacaa
     3421 aaattaaaaa atgaaaacgt acgaattaaa aggcgaagtc agagaagatt tcggaaaaaa
     3481 agcagcgaga agctatcgta gcgaagggct tattccctgt gttgtttatg gcggaagtaa
     3541 cgaaaaaaac ctcaattttg tggtgaaaaa aagcgatgtc cgcaacctca tctacacgcc
     3601 cgaagtttat ttgatcaacc ttgttttgga tggaaaatcc gtaatggcta ttttgaaaga
     3661 gatacagttc catcccgtaa aagacaatat tcttcacatc gatttcttgc acgtattccc
     3721 caatgttccg cttgtaattg aactcccggt tcgcttggaa gggttggctg ccggtgttaa
     3781 gtcgggaggt aaactgtcgc tcgacttgcg taaactgaaa gtgaaagcat tggctgaaaa
     3841 gctgccccag gaattggttg tcaacgtaga agatctcgaa ttgggaaaat ccattcaggt
     3901 gggcgagttg aactttgaag gactggaact gctgaccccg aaaaatgccg ttgtttgccg
     3961 cgttcagctg acacgtgccg ctagaggtgc cgcagctaaa gcacagtaac ggtaaagaga
     4021 aaacgttaaa tgaagtattt aattgtggga ttggggaata tcgggcctga ttacgagaac
     4081 acccgccaca acataggatt tagggtattg gacgcttttg caaaagcgtc caatattgtt
     4141 tttcaggaca agcgatacgg ctttgtcgcc gagacccggt tgaagaataa atcgcttctg
     4201 ctgctaaaac cttccacgtt tatgaaccta agcgggaatg ccgtccggta ttggctgcaa
     4261 aaagagaaaa ttgaagacag gaatctcctc gtagtggtag atgacctggc tctgccgttc
     4321 ggtacactgc ggttgaagtc gaaaggcagt gatgccgggc ataacgggtt gaaacacatt
     4381 gagcaggtcc tgggtactca gcaatacgcc cggttgaggt ttgggatcgg aaatgatttt
     4441 ccccgcggag cacaggtgga ttatgtgttg gacgagtttt ccggtgaaga gaagcagttt
     4501 cttcccgaac gattggagaa ggctgcggaa attatacgca gtttttgcct ggccggcgta
     4561 aatattacga tgaatcagta taataataag taagtgaggt tgtatcaaaa aggatgaacg
     4621 aagttcggat cgataaatgg atgtgggctg tgcggatttt taaaacccga acggttgcgt
     4681 tggatgcatg caagaaaggg cgtgtgttga tcgataacgt atcggtgaaa ccgtcgagga
     4741 tgattcgtgt cggggatgtc gttcaggtac gtaagccgcc ggttactttt tcgtttaaag
     4801 tgctgggttt atccgataaa cggatggggg caaaacttgt cccgcaattt atggaaaatg
     4861 ttacccctcc tgagcaatac gaaatactgg aattgaacaa gataagcgga ttcgtggaca
     4921 ggcagcgcgg aaccgggcgc cccaccaaaa aagaacgccg cgatctggac cagtttaccg
     4981 ataaattcga ttttgatgag tttgactggg atgaataaaa gctattgcaa cactgtaagc
     5041 tttttacttt ccctgttgta atacggcacc accgagaccg tattccgctg gaggcaatag
     5101 gccgcatcgc cctctgcgcc gttggcgatg aggcggtggt aatggtcggc gtttttcaga
     5161 taatccatcg gggagtttct ggctttttcc cataactcga ttgctatccg cacagcgtct
     5221 gatccaaaaa caattttcgc cctttccgac aattttttca caaaggctcc gccaaacaac
     5281 gtgtcttcca tacttacgca gttgttccag ccggcacata agaccacaat tctttcaccc
     5341 gacgacaagc aggtgtcggc caggttgtct atgttggaaa acgcgccgat aaaaagccgt
     5401 ccgcaatctt ttgcagcctc tatagccctc gtgccattgg ttgtggtgaa aacaatatcc
     5461 cgtcccgaca ctttatccgg cgtatattca aacggggagt ttccaaaatc ggcaaactcc
     5521 acgcgacggg tttttctttc agcccctacc aggaatccct ctgatttgta cctctttgcc
     5581 cgttcgatgt cggcaacggg aattatggag gcagctccgt tattgaaagc agcacacatc
     5641 gtggtggtgg cccggaatat atcgactacc acaaccacat cgttctcctt ctgataatag
     5701 gggtaaaggg cgggagagag gcaaatatca acaatcatag acaatgagtt tcgtgcaaat
     5761 ttacaaaaaa caaactttgt tttgtaactt tgttgaaaat ttagggaatg gccattgcaa
     5821 attatcacct tatcctcgcc tccaactctc cccggcgaaa agaactgctt accggtatcg
     5881 acattgagta cgaaataaga acgttgcccg atatcgaaga aacatacacc aacgatactc
     5941 ccccggagaa ggtggcggaa taccttgccc ttaaaaaaac aaccgcttac ctgccacagc
     6001 ttaaagacaa tgaactcctg attacggccg ataccgttgt cctgctgaac aataaagtct
     6061 acgggaaacc gatggataat gccgaagcaa agcggatgct ccgagaactt tcgggccaga
     6121 cacaccgggt catcacgggg gtttgtctta catccacgaa aaaacaagtg gctttttccg
     6181 acactacccg ggttactttt accgaattat cagaagagga gattgaatac tacgtggaaa
     6241 gatacagccc gttggacaaa gccggcgctt acggagtgca ggaatggatc ggctacgtgg
     6301 cggtaaaaca tattgaaggc tcttatttta acgttatggg gttaccgatt caccgtgtgt
     6361 atgaagaatt gaaatcgagt ttcgggtttc gggttgccgg ttaagaaaaa aatattctat
     6421 ctttgcattt tccaacaaat cataaaactt gtctgtccct gacggattta aaatcgcaaa
     6481 caaaacgatg cttacagtag aaaaaatcgg cggaacatcc atgtctcagt ttcaggatgt
     6541 gcttcaaaac atcataaaag gccatcgcaa aggtgatgag tattacaacc gtatatttgt
     6601 agtttcggca tacaacaacg tgaccaattg gttgctggaa cataaaaaga cgggtgagcc
     6661 cgggatttac aacaagtttt tgaacgggga agattacagc agtgcactgg actcgttctg
     6721 tcaacggctc ttactgatca acgatggatt tgccgacatt ggcctggact tgaaagaagc
     6781 gcgtgatttt atccggtttc gagtggacca ggcaaaaacc ctgctctaca gcttgggaaa
     6841 agtacttgcg tcgggatacg taaacatgac tgacatccac ctggcggcca gggaaatcct
     6901 ggcttccctt ggagaagccc attcgggatg gaattcggtc aacatattga acaataacgg
     6961 tataaatgcc cggtttatcg acctttgcgg tttcaacgac aatgagccgc tgaccattga
     7021 ccagcgtatc catcgcgaat ttgcaggagt cgattttagc aatatcgtct gtgtggcaac
     7081 aggatatacg aaaggaacag aaggtattat gcgcgcattt gaccgcggat attcagaagt
     7141 aaccttcagt aagatcgccg ttgaagtgaa agcagatgaa gcggttatac acaaagaata
     7201 ccacctttcc agcgccgatc ctaaaattgt tggggtagaa aacagtgttc ctgttggaca
     7261 aaccaattat attattgccg accagctggc agacatcggc atggaggcta ttcacccgaa
     7321 agctgcgaag cccctggagt tggctggaat caacatacgc attaaaaaca ctttcgaacc
     7381 ggaacatccg ggaacattga tttcacggga ttatatttcg cccgaggcgc gtgttgaaat
     7441 cgtttcgggg tcacgcaaag tgatcgcttt ggagatccac gatccgatga tggtaggcga
     7501 agtgggttac gatgcgcgaa tcatgcagta tttccaaaaa tacaatgtga gctacatcct
     7561 gaaatctacc aacgccaatt ctatcactat ggtggtctgg gatgaagaga gggctcacga
     7621 acttatccgg gtactcaagg aggtatttta ccaggtaacg gtgttgccgg ctgccattgt
     7681 ctgtgccatg ggaacgaaca ttgctatgcc tggattcctt tatcgggcgg caaaggcgtt
     7741 gcacgaaaag ggcataaacg tagagagttt tgcccaatca ctgatgcagg tgaatatgca
     7801 gtttgtcatt tcccgcgagc aatacgaaga gggagtgatc gccctgaacg aagcgttgtg
     7861 taaaagatag tttgccgtaa tgaaagcata tctgtcgcgc cggaaagaac gatttttctt
     7921 tttctttctt tacagcatgt ccctattctt tttcttttct tgtttgaatc cacaggaaaa
     7981 aaaagaatcg gagaatggcc tgttaagtga agaggcgttt aaaacgccag acagggagta
     8041 ttaccccgaa acgtggtatc attttatcgg tggaaacgtt tccaaaccgg gaataaccgc
     8101 cgacctggaa gctatagcta aagcggggat ctctggcatt cagttgttcc atggccagtt
     8161 cggaggagaa tggcccggtg tttcgcctca gattcagacc ttaagtgaag attgggacga
     8221 acttgtacaa tggacggctg aagagtgcaa aaggttgaat ctgcggttta ccatgcaaaa
     8281 ttgtcccggc tggtcgtatg ccggtgggcc gtggattgaa ccggagaatt ccatgcggc
//
LOCUS       DAOSDR010000085         8263 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrimonas sp. isolate AMR_MDS_1826
            NODE_3214_length_8263_cov_6.472831, whole genome shotgun sequence.
ACCESSION   DAOSDR010000085 DAOSDR010000000
VERSION     DAOSDR010000085.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863355
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrimonas sp. (wastewater metagenome)
  ORGANISM  Petrimonas sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Dysgonomonadaceae; Petrimonas.
REFERENCE   1  (bases 1 to 8263)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8263)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:10:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,720
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 35
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8263
                     /organism="Petrimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3214_length_8263_cov_6.472831"
                     /isolate="AMR_MDS_1826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2023866"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1687)
                     /locus_tag="PLI30_04830"
     CDS             complement(<1..1687)
                     /locus_tag="PLI30_04830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI78914.1"
                     /translation="MTEPNKRCVRATLLRWVGLLVMVVACSAVYAQPPAATLDQIRNG
                     PYDAPISPANWVNGNVNSSQAHMVEGYSLPYRVAMTNMPVGTVVTLILEYDTRHSGAN
                     AIDFLTGYNNLEPHLEFWGHPAEVIDPLLGYPQLASATENQIPIDPPQNMPTNTFWNN
                     FMTANPSLNYMSLWGADPFGDSFEYVSEGPLTGNVSSTRFLVRFTVQQSTVVLSWGGH
                     IAKWDDWEPFGEISAGGISGSPYHTRTINWDIPKNNLGNQDRSMQAAVVLKYFTCEVN
                     VTETTCVNSMVYASNIDIDLDIPPDLPNIYEWEIINQVGTNASPLTGTGMAFEFNAGP
                     TPGTLTLRHWVRAPYTNTYISKFCDYPIMVNPGPMCSITPLPDADLCPGSTQTYYAPD
                     GAYTYLWTITGNGTIDGSTTGQSVSVIAGDDCGASYILTLKITDAALCESTCSETYMV
                     DDDVPPVIIDLEDIMLQGCNTAWPAAPTTTFTDNCTEPYTLTGVPGDVMTDGCYQYID
                     YAFNVTDDCDNEATEVIVRVTRMYDETPPVIVDIDDIMLQGCNTAWPAAPTTTF"
     gene            2519..3049
                     /locus_tag="PLI30_04835"
     CDS             2519..3049
                     /locus_tag="PLI30_04835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI78915.1"
                     /translation="MKKLFIFICWSAVSVTYAQFVAQPLNYPGMGYWPYYFSIADPEH
                     VWIGTIHESGIPYSMAVHTDNGGESWIFDSIPVPGIPFITSTASLDSNTCFYVFSDFN
                     VGGGSIWKTDDAGDNWSCLTTTQFDGGFANFYILSLPIPGLRSGIRWMAISRYSLPAT
                     VETPGKDCQQSIFLNP"
     gene            2962..3828
                     /locus_tag="PLI30_04840"
     CDS             2962..3828
                     /locus_tag="PLI30_04840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI78916.1"
                     /translation="MDGYFEIQLTCDGGNTWERLPAVNIPEPIDGEYALTNCYCTYGN
                     SIWFSTTKGRCFRSVDRGRHWDATRVVTTVSQSFNVCFSSLLTGAFWSMESNNGDIAI
                     TSDGGVSWDTLAFMPGHIILHMSNVEGFEEGFVVSTWKNSTNVYFTANMFESYQVLGH
                     NMISTGALDFIDETTGWLSGGETGSYEIYKFMGVLTKTRSIAATGELIQIHPNPASIY
                     SDVIIPPKLISNNSELNIRGITGQLIEGIPLNGDIRRLRLDADKYENGLYLIQIISDN
                     APVFSRKWIVAH"
     gene            4002..6212
                     /locus_tag="PLI30_04845"
     CDS             4002..6212
                     /locus_tag="PLI30_04845"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOI78917.1"
                     /translation="MRNLIVFSLICLSSHIYPQTADWQYYAKDNSINSLTLDNDNLWV
                     GLNSGLLKLNTIDGSKAFYNRINSPLPESWISDVAVDGNGIKWITTWIKGTLLRFDDN
                     QWIIYDEYNSPLPGNGCSLTNVEIGNNNDIWLSTACINTGLIKFDGVNWMVYQTSNSG
                     IPSNYIFAMYCSGNKVWMTSSLDLFSFDGTNWTIYNTENSNISGHTIFDIDEDSQGNI
                     WLLHGNGVEKFDGHTFTLFDNSNTNIPNINNYKFDIDSQDNIWTTCISYGFSPEILGG
                     IMKFDGVQWTKFDTLNADIPDIDISPILIGPSDELWYGCANLGMVGKKTGDLWTVFDP
                     SNSGLHHSQVRQIVNGTGHATYIGTSQPKLSGSALVGFDWGSWIDLPYYGSNTYKMTI
                     STDGKLYIKNRDGIRYYQNGIWHLIPDPPTLYAPSVPLGLSAMSADAAGNIWIDYLSG
                     VETYFDPNTGQYYYVAKEGLARFDGNSWTTFTPENSPIPDADISQIQTDLNGNTWIGT
                     NQGLLLNTGNDWLVYNTSNSEIPFNNIYSFAVDSSGSVWFPDCNLGFYRFDLLHSEHF
                     PYPGISKDRYTGEVVLDIDGTPWQTGLFSLIHFDGKEWEEFNAENAPLPYGNVLSLSI
                     DQNGNKWIGTQFGVMVYKAGGVITSSNSPVPEGNNYTIFPNPFSEYFEIQFDNIPGKV
                     IISIYNTKAQLVCETTGNSGSKVMVPRKNMHPGLYFYRISTGGKLVAAGKIVAL"
     assembly_gap    6417..6516
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            6629..6702
                     /locus_tag="PLI30_04850"
     tRNA            6629..6702
                     /locus_tag="PLI30_04850"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6663..6665,aa:Asn,seq:gtt)
     gene            6993..8107
                     /locus_tag="PLI30_04855"
     CDS             join(6993..7265,7268..8107)
                     /locus_tag="PLI30_04855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611896.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HOI78918.1"
                     /translation="MRKKRQNYSAEFKAKVAIEALKEQKTIVELAQLFEIHPNQISTW
                     KKEFLDNAAQVFDKLHRKPKENEEVAKLFEKVGQLTIENDWLKKKLLGCTIEQKRAMI
                     DKGHTKLSVQKQCELLSIHRSGVYYKPCEETPLNLDLMKVIDREYTRLPFYGVPRMTE
                     HLRQFGFEVNEKRIRRLYRLMAIRAIYPSPKTTISNKEHFKYPYLLRNLKIDRPNQVW
                     ATDITYIPMRHGFMYLVAIIDVYSRFLLNWSISNTLDSEFCVEALKEAIQQYGKPEIF
                     NSDQGVQFTCKEFIEVLRKNEINISMDGKGRAIDNIFIERLWRSVKYENIFLNAYTDG
                     MELYRGLKSYFEFYNNERLHQSLDYQLPGKIYREAA"
BASE COUNT         2384 a         1734 c         1832 g         2213 t
ORIGIN      
        1 taaatgtggt ggtcggtgca gcaggccatg cagtgttgca gccctgaagc atgatgtcat
       61 caatgtcaac aatcaccgga ggagtttcat cgtacatgcg ggttacacga acaataactt
      121 cagttgcttc attgtcgcaa tcgtctgtta cgttgaacgc ataatcaatg tactgatagc
      181 aaccgtcggt cataacatct cccggaacac cggttaaggt atacggttcg gtgcagttgt
      241 cggtaaatgt ggtggtcggt gcagcaggcc atgcagtgtt gcagccctga agcatgatat
      301 cctccaggtc aataatcacc ggcgggacat catcatccac catatacgtt tccgaacagg
      361 tgctttcgca taatgcagca tcggtaattt tcagcgtcag tatataagac gcaccacaat
      421 catctccggc tataaccgaa actgattgtc ctgttgtcga accatcgatc gttccattac
      481 cggttatagt ccataaatag gtatatgcac cgtctggcgc ataatacgtt tgtgtggagc
      541 cgggacaaag atctgcgtct ggcaatggag taatcgagca cattggaccc ggattaacca
      601 tgataggata atcacaaaac ttagaaatat aggtattggt gtacggggct cttacccaat
      661 gtctcagtgt aagagttccc ggtgtgggtc cggcattgaa ttcaaaagcc attccggtgc
      721 cggtcagtgg actggcattg gttccgacct gattgatgat ttcccattca taaatgttgg
      781 gaagatcggg aggaatgtcg agatcaatgt caatgtttga ggcataaacc attgaattaa
      841 cgcatgtagt ctcggtaaca ttaacttcgc aggtaaaata ttttaataca acggcagcct
      901 gcatggaccg atcctggtta cccaggttat tttttgggat atcccagtta atggtacggg
      961 tatggtaggg agatccgctg atacctccgg cagagatctc accgaaaggt tcccagtcgt
     1021 cccatttggc aatgtgtccg ccccaggata gaacaacagt agattgttgt accgtgaacc
     1081 taaccagaaa acgggtagag gatacattgc ctgtcagggg cccttccgaa acatattcaa
     1141 aggaatcgcc gaagggatca gctccccata acgacatgta gttaagtgag gggttagccg
     1201 tcataaaatt attccagaag gtattggtcg gcatattctg aggaggatca ataggaatct
     1261 gattctcagt tgccgatgcc aactgaggat aacccaaaag cggatcgatc acttcagccg
     1321 ggtgtcccca gaattccaag tgtggttcaa ggttgttgta tccggtgagg aagtcgatgg
     1381 cgttggcgcc gctgtgcctg gtatcgtatt ccagaatcag ggttacaaca gtacccaccg
     1441 gcatattggt cattgccacc ctgtagggaa gcgaataacc ttcaaccatg tgtgcctggc
     1501 tggagtttac gttaccgttt acccagttgg caggagaaat gggcgcatcg tagggcccgt
     1561 tacggatctg atcgagggtt gcagcaggcg gctgcgcata aacagcgcta cacgccacaa
     1621 ccatcacgag caaccccacc cacctcagga gggtcgctct tacacaacgt ttgtttggtt
     1681 ctgtcatagt taattattta agggttatta attgggttta ttttgtggta ttgcatacgt
     1741 tttatgcata cctggcatca caaaagaatc acaaacgatt aacgaaacga ccttccagcc
     1801 ctctggcaaa gggttatcag ggatcatgcg taaaaaccgc tgatctgaag attcagctgg
     1861 tgttttacgg gttaatcaga tgttattttc tatggatttc tgcccataga atgcaggtgg
     1921 ggtttggttt gaatccccgt gccagaacca gctcaggggt atgctgacta taattcagat
     1981 agggcaggca gttatgagca atcagaatag aagaaggaaa attgcacaat acagttcaat
     2041 ggctattttc tattaaatcc atccaatgcc tgatcaggcg taatggtgcc cggaagacct
     2101 tttaaatgga atgtaaaggt acaatacgtg atgcggccat aaaagtgtaa attaatgtat
     2161 tttgactacg tttttttacg cagttgggat gttcgccgga gaataaaccg aaagcaattt
     2221 atttctaaaa atctgtttta caatatttta aaaaaatact gcgcaaagta cctggaattt
     2281 taaacaccac tgactcccgc agaaaaatga aataagtctg tacagctggc aagtttcagc
     2341 atcgttaaaa aatggttcag caacttcaaa atgccttttg gtcttttccg gaatgatgtc
     2401 ttccaggcag gcactaaaac tgcttaaggt ttattataac aaggaattgc ttttttcata
     2461 aatatttctt aacttcattg gttccgttcc ggttttggat ggaataaaaa gtaaggccat
     2521 gaaaaagctg ttcatcttta tctgttggag tgcagtctct gtgacttatg ctcaatttgt
     2581 ggcccaaccg ctcaactatc ccggcatggg ttactggccc tattatttct ccatcgccga
     2641 cccggaacat gtgtggattg gcaccattca tgagtccggc ataccctact cgatggccgt
     2701 ccatactgat aatggaggtg agtcctggat ttttgactcc attccggtcc ctggtatacc
     2761 atttataaca tccactgcct ccctggattc caatacctgt ttttatgtgt tttccgattt
     2821 caatgtggga gggggtagca tctggaagac tgatgatgcc ggcgacaact ggtcatgcct
     2881 taccaccacg caatttgatg gcggctttgc caatttttac atcctttcac tgccgatacc
     2941 ggggttgcgg tcggggatcc ggtggatggc tatttcgaga tacagcttac ctgcgacggt
     3001 ggaaacacct gggaaagact gccagcagtc aatattcctg aacccataga cggggaatac
     3061 gcattgacaa actgttattg tacctatgga aactcgatat ggttctccac aaccaaaggg
     3121 cgatgcttca gatctgtaga caggggccgg cattgggatg caacacgagt ggtaacaacg
     3181 gtaagtcagt cattcaatgt ttgtttttca tccttgctga caggagcatt ctggagcatg
     3241 gaatccaaca atggggacat tgccatcaca agtgacgggg gagtaagctg ggacacgctt
     3301 gcattcatgc ctggccatat catactgcac atgagcaatg tagaaggatt tgaagaggga
     3361 tttgtcgttt ccacctggaa aaattcgacc aatgtctatt tcacagccaa tatgtttgaa
     3421 tcctatcagg ttttgggtca caatatgata tccactggcg ccctggactt catcgatgaa
     3481 acaaccggct ggttgtcggg aggtgaaacc ggcagctacg aaatctacaa attcatgggc
     3541 gtcctgacaa aaacccggtc gatagcagcc accggcgagc tgattcagat acatcccaat
     3601 ccggcatcca tatactcgga tgtgatcatc cctcccaaac tcatctccaa caactccgaa
     3661 ttgaacatca ggggaataac ggggcagctg atagaaggca tacctcttaa tggtgacatc
     3721 cggaggctga ggttggatgc ggacaaatac gaaaacggat tgtacctgat ccagattata
     3781 tccgacaacg ccccggtttt tagcaggaag tggattgtag cgcattgata gttggttatt
     3841 cgaaacacct gaagtgcaat cttcgggatt aacaaggctt cattagtcca taagtacttt
     3901 tattgattcc tctgccccat tgtaatatag aaaaattata ctcacatttg caatgagtcg
     3961 ttttcgccat gtattgccta cctcatgtaa aaaagaatcc tatgagaaac ctcattgtgt
     4021 tttcattaat ttgcctatcc tctcacattt accctcaaac agccgattgg caatactatg
     4081 caaaagacaa ctcaatcaat tcactgacac tggacaatga taatctatgg gttggattga
     4141 actcgggatt gttgaaatta aatacaatcg acggaagtaa agccttttat aacaggatta
     4201 actccccgct tccggaaagc tggatttctg atgtggcagt tgacggaaac ggaattaaat
     4261 ggattaccac ctggattaaa ggcacccttc ttcggtttga tgacaatcaa tggatcatct
     4321 acgatgagta caattcccct ttaccgggga atggctgctc cctcacaaat gttgagattg
     4381 gcaacaacaa cgatatctgg ctttctactg catgtatcaa cacagggctg ataaaatttg
     4441 acggagtgaa ctggatggtt tatcaaacct caaactcagg aattccaagc aattacatct
     4501 ttgccatgta ctgttccgga aataaagtat ggatgacctc ctctcttgat ctgttttcat
     4561 tcgatggaac caactggacc atttacaata cagaaaattc gaacatttcc ggtcatacta
     4621 tctttgatat tgatgaggac agtcagggga atatttggct cttgcatgga aacggagtag
     4681 aaaaatttga cggccacacc ttcaccctat tcgacaattc aaacaccaac atacccaata
     4741 tcaacaacta taaatttgat atcgattcgc aggacaatat atggaccact tgcatttcgt
     4801 acggtttttc accggagatt ctggggggga taatgaaatt tgatggagta caatggacta
     4861 aattcgatac actgaacgct gatattcccg atattgatat ttctcctatc cttattggcc
     4921 cctctgatga attgtggtat ggatgtgcca atcttggaat ggtaggtaag aaaacgggag
     4981 atttatggac tgtctttgac ccttcaaatt caggtttgca ccatagtcag gtcaggcaaa
     5041 tcgtcaatgg aaccggccat gccacttaca ttggcacctc acaacctaaa ttgtcgggca
     5101 gcgcgcttgt cggttttgac tggggaagtt ggattgacct tccatactac ggatcaaaca
     5161 cttataaaat gaccatttcc actgacggaa agctatatat caaaaatcgt gatggaatca
     5221 ggtattatca aaacgggata tggcatttga tacctgatcc tcccacacta tatgctccat
     5281 cggttccgct gggcctctct gctatgagcg cagatgccgc cgggaatatc tggatagatt
     5341 atctttcagg ggttgaaacg tactttgacc ccaacaccgg tcaatattat tacgtggcca
     5401 aggaaggtct ggcccgattc gacggaaatt catggactac attcacgcct gagaactccc
     5461 ctattccgga tgctgacatc agtcagatac agacagactt aaatggcaac acgtggattg
     5521 gaaccaatca ggggctcctc cttaacacag ggaatgactg gttggtttat aacacttcca
     5581 actcggaaat acccttcaat aacatctact catttgctgt ggattcatcc ggctcagtat
     5641 ggttccccga ctgcaactta gggttttaca ggtttgatct cctgcattca gagcattttc
     5701 cttacccggg aatatcaaag gacaggtata caggcgaggt agttcttgac attgatggta
     5761 caccatggca aaccggattg ttttcattga ttcatttcga tggaaaggaa tgggaagaat
     5821 tcaatgctga aaatgctccc ttgccatacg gcaatgtact ctccctttcc atcgaccaaa
     5881 atgggaacaa atggattggt acacagttcg gtgtaatggt ttacaaggct gggggtgtga
     5941 taacatcctc caattctcct gtgcctgagg ggaataatta tacaatcttc cccaatccgt
     6001 tttcagaata ttttgaaata caattcgata acattcccgg caaagtcata atcagcattt
     6061 acaatacaaa ggctcaactg gtctgtgaaa caacagggaa ttcaggctca aaagtcatgg
     6121 ttccccgcaa gaacatgcat ccgggactat atttttacag aatcagtaca ggaggcaagt
     6181 tggttgcagc cggaaaaata gtagcgttgt aaatggatcc cggagaaagg cggccggcaa
     6241 tcagcccgat tccaaggata agtgaccata ttaatggttt attttatttt acagaaatat
     6301 tttagcatgt ctcttgcaaa ttagaaaata tgatttaatt ttgcagtccc aattaaggga
     6361 aaattcctcc ttagctcagt tggttagagt cccgcaactg cgggattaat cagaggnnnn
     6421 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6481 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnngccg gcggaatttg aaatagagtg
     6541 ttatactcct ccttagctca gttggttaga gtcccgcaac tgcgggatta atcagagggc
     6601 cggcggaatt tgaaatagag tgttatactc ctccttagct cagttggtta gagcatctga
     6661 ctgttaatca gagggtcgct ggttcaagtc cagcaggggg agcaggaaaa gagccgctaa
     6721 acgcggcttt tttactttta tgtactatat ctacatccta tactctgaaa cctcggatct
     6781 ctattacatc ggttactctg acaatgttga cagaagactt tctgagcata ataacgcacc
     6841 ttataatacc ttcacatcca aacaccgccc atggaagata aaagcagttt tctccgtttc
     6901 cggtgacatt ggaacggcaa tgaatataat actccccaaa tttaggacag cgagttaagg
     6961 tgtaaaaaca aacccttaat tttactgtcg ctatgagaaa aaagagacaa aattacagtg
     7021 cagaattcaa ggcaaaagtt gcaattgaag cactaaagga acagaaaacc attgtcgaac
     7081 tggctcagct ttttgagatt catcctaacc agattagtac ctggaagaag gaatttcttg
     7141 ataacgcagc acaagtattc gacaagttgc accggaaacc gaaggaaaat gaagaagtgg
     7201 ccaaactctt tgagaaagta ggccaactga ctatagagaa cgattggtta aaaaaaaagc
     7261 tactgtagga tgtaccatcg agcaaaagcg cgccatgata gataaaggtc atacgaaact
     7321 aagcgttcaa aagcagtgtg aattgctttc aatccatcgt tccggggtct attataagcc
     7381 ttgtgaggag actcctttaa acctggatct aatgaaagtg atcgaccggg aatataccag
     7441 attgcccttc tatggagttc ccaggatgac agagcattta aggcaattcg gctttgaagt
     7501 taacgagaag agaatacgaa ggctttatcg gctaatggca attcgggcaa tatacccttc
     7561 tccaaaaaca acaatttcga acaaggaaca cttcaagtat ccatatctgt tgagaaatct
     7621 taaaattgat cgccctaatc aggtctgggc tacagatatc acctatattc ccatgaggca
     7681 tggattcatg tatctagtag ctataattga tgtttacagc cgctttctat tgaattggag
     7741 catctccaat acccttgact cggaattctg cgtggaagcc ttaaaagaag ctattcagca
     7801 atatggtaag ccagagattt ttaattctga tcagggagta caatttacat gcaaggaatt
     7861 tatcgaggtt cttcgaaaga acgaaatcaa tatcagcatg gatggcaagg gacgagcgat
     7921 agataatatc ttcattgaaa gactttggag aagcgttaag tacgagaata tcttcctgaa
     7981 tgcttacaca gatggaatgg agctttacag gggtctcaaa agttattttg agttttacaa
     8041 taatgaaaga ttacaccaga gtcttgatta tcagttgcca ggcaagatct atcgagaggc
     8101 tgcctgagtt ataaacaaat taacagaaat gaacaaagaa aaaaagaagc aaaaaaagaa
     8161 aagtcttgat acgggaaata actcttcgag ttatttcccg tatcaatcct attaaacaac
     8221 aatttacttt aacttttttg tcctaaaaat ggggggaagt ata
//
LOCUS       DAOSDR010000086         8232 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrimonas sp. isolate AMR_MDS_1826
            NODE_3230_length_8232_cov_4.057967, whole genome shotgun sequence.
ACCESSION   DAOSDR010000086 DAOSDR010000000
VERSION     DAOSDR010000086.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863355
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrimonas sp. (wastewater metagenome)
  ORGANISM  Petrimonas sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Dysgonomonadaceae; Petrimonas.
REFERENCE   1  (bases 1 to 8232)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8232)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:10:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,720
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 35
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8232
                     /organism="Petrimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3230_length_8232_cov_4.057967"
                     /isolate="AMR_MDS_1826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2023866"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..380)
                     /locus_tag="PLI30_04860"
     CDS             complement(<1..380)
                     /locus_tag="PLI30_04860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019541268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HOI78919.1"
                     /translation="MKTKISFLTVSVLIFTIIVSSCNPKKQNYSYFQNSGEIFHTSYH
                     IKYAYKRSLEDEILAELDRFEQSLNPFRENTVITKINSNIPVKPDSLFMEVFNKATEV
                     SRMTDGKFDITASPLIDAWGFGFR"
     gene            complement(407..922)
                     /locus_tag="PLI30_04865"
     CDS             complement(407..922)
                     /locus_tag="PLI30_04865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006798758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HOI78920.1"
                     /translation="MKLDEIIQLRRSVRKFKDQSIDKEVIGEIIESARLAPSAVNFQP
                     WKFYICRSEEAKKIVRQSYPREWFNSAPLYILACGDHQQSWKRSCDGKDHLDIDIAIA
                     VEHMVLKITELGLGTCWVCNFNPQTVKEGLQLSENEEPIVLLPVGYPENVDPDPRNKK
                     RKSPEEITKWI"
     gene            complement(933..1883)
                     /locus_tag="PLI30_04870"
     CDS             complement(933..1883)
                     /locus_tag="PLI30_04870"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019541269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOI78921.1"
                     /translation="MDISVVIPLYNEEESLPELHAWIKRVMEQNGFSYEIIFVDDGST
                     DHSWEVITGFREQSDRVKAIKFQRNYGKSPALHCAFRKVKGDVVITMDADLQDSPDEI
                     PELYRMIREEGYDLVSGWKRKRYDATLTKNLPSKLFNATARKVSGIRLHDFNCGLKAY
                     KRSLAQSIEVYNDMHRYIPILAKDAGFKQIGEKEVRHQARKYGKSKFGADRFVNGYLD
                     LLTIWFLNKFGKKPMHFFGLLGSIMFILGFIASILVGATKLYDMHVGNPYRLVTESPY
                     FYISLTMMILGTLLFLTGFLGELISRNSPDRNNYRIEEEF"
     gene            complement(1903..2436)
                     /locus_tag="PLI30_04875"
     CDS             complement(1903..2436)
                     /locus_tag="PLI30_04875"
                     /inference="COORDINATES: protein motif:HMM:NF025227.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4199 domain-containing protein"
                     /protein_id="HOI78922.1"
                     /translation="MQEDKSQLMNYAMYGGVFLGLFWMLKYLFVMGAGRIPALSILGS
                     FLAIGTPLILFYFLVKYKTAIVNNNMGYGHGVQFGIMLFFFASLLEAVIVFVHITWVD
                     QAFVGKLYENMIETVRMMNFSESMVNSLEDQPLPTTVNYIFSNVILADVFIGTILSLF
                     VVPFARRYTPANRKSSI"
     gene            2596..3807
                     /locus_tag="PLI30_04880"
     CDS             2596..3807
                     /locus_tag="PLI30_04880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019541300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-xanthine permease family protein"
                     /protein_id="HOI78923.1"
                     /translation="MENVKPLTPLSKTVVGVQFLFVAFGATVLVPLLVGLDPSTALFT
                     AGIGTLVFHLVTKGIVPIFLGSSFAFIAPIVKATELYGLAGTLSGLVAVGAVYFLMSA
                     LVKWQGMKVIQRLFPPVVIGPVIMLIGLSLSGTGVNMAAENWLLAIIALVTAIIVSMF
                     AKGLLKLIPIFSGIIVGYIAAVFMGKIDFTPVIEAPWFSLPQFVTPKFSWQAILFMAP
                     VAIAPVIEHVGDVYAVSQVAGKDFVKKPGLHRTMLGDGIACSIAGLIGGPPVTTYSEV
                     TGAMVLTKVTDPVVIRIAAVTGIVFSLIGKISFFLKTIPNAVLGGIMLLLFGMIAATG
                     VNNMIANKTDMSVTRNLIIVSLILTTGIGGAIFKIGDFTFAGIGLAAMVGVVLNLILP
                     GHKEEKGSEAK"
     gene            3936..4529
                     /locus_tag="PLI30_04885"
     CDS             3936..4529
                     /locus_tag="PLI30_04885"
                     /inference="COORDINATES: protein motif:HMM:NF025273.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HOI78924.1"
                     /translation="MEKKKTQKTNKKGSWIFNIIFIGLIALILFTPVGSKLKYWTSKL
                     MASFTPSVQKVEKREKLTDYHWLLTDNNSQSFDLSQAQGKVIFLNTWATWCPPCIAEM
                     PALQELYNKYKNNPDVVFVFATTDPKNTVDKFMTDKGYNLPVYYIQSAPPPQLASNTI
                     PITFLIGKNGGIAIRKVGAADWSSRKVTGTIDQLLQE"
     gene            complement(4561..5541)
                     /locus_tag="PLI30_04890"
     CDS             complement(4561..5541)
                     /locus_tag="PLI30_04890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005795082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOI78925.1"
                     /translation="MINWGFIGCGNVTEKKSGPAFRKVDGSDVVAVMRRDAESAKDYA
                     LRHHIAKWYSDADALINDSAVNAVYVATPPGSHAEYAVAAMKAGKPVYVEKPMAASFE
                     ECVIMAEAAKETGVPCFVAYYRRTLPYFLRIRQLLESGEIGEVREVKIRFIIPPYPSD
                     LSRETLPWRVKKEVAGAGYFYDLASHQLDYLDFVFGEITEVGGTAKNVAGLYDVEDTV
                     DGEWLHKTGVRGSGHWEFAAPVSERTDEVEIIGSKGSIRFSTFHFTPIVLNRASGEKI
                     YTEENPENIQFYLIQSIVNYLNGNGPYPVGDFRSAMRTNWVMDKMLGRIP"
     gene            complement(5545..7986)
                     /gene="ccsA"
                     /locus_tag="PLI30_04895"
     CDS             complement(5545..7986)
                     /gene="ccsA"
                     /locus_tag="PLI30_04895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010802814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
                     /protein_id="HOI78926.1"
                     /translation="MEKIRQLLASYKTTIVLLLVYAFLMALATFIEKEMTTEAAKVMI
                     YYSPVFFFLQFLLVVNFILTFIDHRFIKRRRWALTVIHFAFVVILAGALTTHVFGKEG
                     TMHIREGETNNVMVMHTSKGVFKEELPFTLELTQFRLIRYPGSMSPSSYESDLRIHID
                     GEVREARVYMNNVLDLKGYRFFQASYDEDEQGTVLSVNKDVAGRNITYAGYFFLVAGF
                     VLMFVTPNSRLNKLSRQLNELRRSAKLTAVFLLVTFSAGAQDFTSQAVFQAVQRNAVP
                     VEHAAEFGQLPVQFAGRIIPMNTFSAELLRKIHKNNKIGRLNSDQFLLGILTMPQMWM
                     QVPFIANSNEEVAKMFQLPEQHFAYAQVFDPKGNYKLLARVNEAYHKPESQRNEFDKD
                     IIKLDEKINILFLLLNHKLLTLFPKADAPNDTWYAPGDDLSGIPEEDSLFISRSLPLY
                     LSEVNRSLESGDWQQPNTILDSIAAFQQKADQAGHINPKKIRTEIRYNKQNIFSKTRT
                     GYFALGLLLLMTAFLRLFKEAMWTNILSKVLVWGIFLVFLYHVYGMAMRWYISGYAPW
                     SNSYETMVYVAWATILAGLIFGRKSDLTLATATIFGGIILFVSGLNWMEPQITTLVPV
                     LKSPWLMFHVAVTVAAYGFFGISLLLGLSNLLILSVAKKETAMLHVRELSIINNMSLL
                     VGLALMTIGTFLGGIWANESWGRYWSWDPKETWALITVVVYSVVTHIHLVRKLNNDWF
                     FNLASVMAFASVLMTFWGVNYLLSGLHSYGENEGVSEVFVYLYAILFGVIVLALVSYR
                     GYKKFKSQGSTSNFY"
BASE COUNT         2269 a         1917 c         1769 g         2277 t
ORIGIN      
        1 ttcctgaaac cgaatcccca ggcatcaata agcggcgagg ccgtaatatc aaattttccg
       61 tccgtcatcc gtgaaacttc cgtggctttg ttgaatactt ccataaaaag tgaatcgggc
      121 ttcacgggta tattgctgtt tattttggtg atgaccgtat tttcccggaa aggattcaaa
      181 gattgctcaa acctgtccag ttcagccaga atctcatcct ccaatgatct cttgtaagcg
      241 tatttgatgt ggtaagaggt atggaaaatt tcaccgctgt tttgaaaata ggagtagttt
      301 tgttttttag gattacacga agatacaata attgtaaaaa taagtacgga taccgttaga
      361 aaagatattt ttgtcttcat ctgtgtttta attaattgaa atatcattag atccacttcg
      421 ttatttcttc cggcgatttc cgtttcttgt ttctgggatc cggatcaacg ttttccggat
      481 aaccaacggg taatagaacg atgggttcct cattttcact gagttgtaat ccctccttta
      541 cggtttgcgg attgaagttg caaacccaac aagttccaag tccaagttca gtgattttta
      601 acaccatgtg ttccacggca atcgctatat cgatatcgag gtggtccttg ccgtcacacg
      661 agcgtttcca cgattgttgg tgatctccgc aggcaaggat gtatagcggg gcactattga
      721 accactctcg cggatagctc tgacgtacta tttttttggc ttcttccgat cggcatatgt
      781 agaatttcca cggttgaaaa ttaacggctg aaggagctaa acgggcactt tcgattatct
      841 caccaataac ttctttatca atagattgat ccttgaattt tcttaccgat ctgcgtaact
      901 ggattatctc gtcaagtttc atctgttatc ccttaaaatt cttcttcaat cctgtaatta
      961 ttccggtcgg gagaattgcg cgaaatcaat tcacccagaa aaccggtaag gaaaagcagt
     1021 gttcccagaa tcatcatcgt tagtgatatg taaaagtagg gcgattcggt taccagccgg
     1081 tacggatttc ctacatgcat atcgtacagt ttagtggcgc ctaccaggat gctggcaata
     1141 aaccccagaa taaacattat actgcccaag agcccaaaga aatgcatcgg tttcttgccg
     1201 aatttattca aaaaccagat ggtcaacaaa tcgaggtaac cgttcacaaa acggtctgcg
     1261 ccgaacttgc tttttccgta tttgcgcgcc tgatgacgga cctctttctc gccgatctgt
     1321 ttaaatcctg catcttttgc cagaatggga atatagcggt gcatgtcgtt atacacttca
     1381 atgctttggg ccaatgagcg tttgtaggct tttagaccgc aattgaaatc gtgaaggcgg
     1441 atacccgata cttttctggc cgtggcatta aaaagcttcg acggcagatt tttggtcagt
     1501 gtggcatcgt aacgcttgcg tttccatccc gaaaccaggt cgtaaccctc ttcccttatc
     1561 atccggtaaa gttcgggaat ttcatcgggg ctgtcctgca ggtcggcatc catggtaatc
     1621 accacgtctc ctttaacttt ccggaaggca cagtgcaaag cgggtgattt tccgtaattg
     1681 cgctggaact taatcgcctt cacccggtcc gattgttcac gaaatccggt aatcacttcc
     1741 cacgaatgat cggtactccc atcgtctaca aagataattt cgtacgaaaa tccgttttgt
     1801 tccatcaccc gcttaatcca tgcgtgcagt tctggaaggg attcctcttc gttgtagagt
     1861 ggaattacaa cagaaatatc cataattttt tatttgacga ttttagattg acgatttacg
     1921 atttgcaggt gtgtacctgc gggcaaacgg cacaacaaat aacgacaaaa tcgttccgat
     1981 aaaaacatcg gccaggatca cgttactgaa gatatagttc acggttgtcg gtagcggctg
     2041 gtcttcaagg gaatttacca tgctctcact gaaattcatc atccttacgg tctcgatcat
     2101 attttcatac agcttcccaa caaaagcctg gtcaacccaa gtgatatgta cgaagacaat
     2161 cacagcttcc agaagtgatg caaaaaaaaa cagcatgata ccaaactgga ctccgtgacc
     2221 atatcccata ttgttgttta ctatagcggt tttatacttt accagaaaat aaaaaagaat
     2281 aagcggagtg cctatagcca agaatgatcc gaggatactt aaagccggaa tccggcccgc
     2341 acccatcaca aaaagatatt tcaacatcca aaacaaaccc aaaaaaacgc cgccatacat
     2401 ggcatagttc atcaactgac tcttatcttc ctgcataaaa aactatttaa cgtacaaaag
     2461 taactaaaaa aagtcagaag tcggaaacca gaagccggaa gattgttttt ttcataaatc
     2521 gcgaatgatt aactaaaaat ttcgataaat acgttaactt tgcgcgaatc aaaaaaagta
     2581 aacatatttt ttagcatgga aaatgtaaaa ccgctcaccc ccctgtccaa aactgtggtt
     2641 ggggttcagt ttctttttgt ggctttcggt gccacggttt tagtacctct tttagtcggc
     2701 ctggatcctt ccacagcatt attcactgcc ggaatcggaa cgctggtctt tcacctggtt
     2761 acgaaaggca ttgtccctat ctttttaggc agcagtttcg cttttatcgc gcccattgtt
     2821 aaagccaccg agctttacgg attggccggg accttatcgg ggttagtggc cgtaggcgct
     2881 gtttattttc tgatgagtgc actcgtcaag tggcagggga tgaaagttat ccagcggttg
     2941 ttcccacccg tagttattgg cccggttatc atgcttatcg gtttgtcgct ttccggaacg
     3001 ggtgtgaata tggctgcaga aaactggctg ctggctatta ttgcgcttgt aactgccatt
     3061 atcgtgagca tgtttgccaa agggttgctg aaactgattc ctattttcag cggaatcatt
     3121 gtgggataca ttgctgctgt ttttatgggg aagatcgatt ttacacccgt cattgaagct
     3181 ccctggttca gtctgccgca atttgtcacc ccaaaatttt cgtggcaagc catcctgttt
     3241 atggctccgg tagccatcgc tccggttatt gaacatgttg gcgacgttta tgctgtgagc
     3301 caggtagctg gaaaagattt cgtaaaaaag ccgggacttc accgaacaat gttgggtgac
     3361 ggtatcgcat gcagcattgc cgggcttatc ggcggacctc cggttaccac ttattcggaa
     3421 gttaccggcg caatggtgct gaccaaagtg accgatccgg tagtgattcg cattgcggcg
     3481 gtgacgggca ttgttttttc gttaattggg aaaataagct tcttccttaa aaccataccc
     3541 aacgcggtgc taggtggaat tatgttgttg cttttcggca tgattgcagc taccggcgtc
     3601 aacaacatga tcgccaacaa aaccgatatg agcgttacac gcaacctgat tattgtctcg
     3661 cttatcctga ctacgggaat cggtggggct atttttaaga taggtgattt tacctttgcc
     3721 ggcatcgggt tagcggctat ggtcggtgtg gtcctgaact tgattttacc cggccacaag
     3781 gaagagaagg gaagcgaagc caaataaaaa acccaataat attgtccgga cagtaacagg
     3841 agccggtgtg tcttgcagat acggagcgga agttcatttt tccgctccgt agaacttttt
     3901 attgaaaaat acgttcaaag ggaaagaacg taaaaatgga aaaaaagaaa acgcagaaaa
     3961 ccaacaaaaa gggatcgtgg atttttaata taatttttat cgggttaatt gccttgattt
     4021 tgtttacacc ggttggtagc aaattgaagt attggacaag caaactgatg gcttctttta
     4081 ctccatcggt gcaaaaagtg gagaaacgcg aaaaattaac cgattaccac tggctgctga
     4141 ccgataacaa cagtcaatct ttcgatttaa gtcaggcgca ggggaaagtg attttcctga
     4201 atacctgggc aacatggtgc ccaccttgca ttgcagagat gcctgcctta caggaacttt
     4261 acaacaagta caaaaataat cccgatgtgg tgtttgtgtt tgcaaccaca gatccgaaga
     4321 atacggtgga taaattcatg accgacaaag ggtacaattt gccggtgtat tacattcaat
     4381 ctgccccacc ccctcaactg gcttccaaca ccattcccat cacttttttg attggaaaaa
     4441 acggcggtat tgccatccgg aaagtaggcg ccgccgattg gagcagcagg aaggttaccg
     4501 gcacaattga ccagttactt caagaataaa acgtaccgct atccggtgag ggcatacctg
     4561 tcaaggtatt cttcccagca tcttgtccat tacccaattg gttcgcatgg cagaacgaaa
     4621 atcacctacg ggatagggtc cgttgccatt caggtaattc acaatgctct gaatcaggta
     4681 aaactgaatg ttttcgggat tttcttcggt atagattttt tcgccggagg cacggttgag
     4741 gacgataggt gtgaaatgaa acgtagaaaa ccggatagat cccttcgatc caataatttc
     4801 cacctcatcc gtacgttcac tcacgggagc tgcaaattcc cagtgtccgc tgcctctcac
     4861 acccgtctta tgcaaccact ctccgtccac cgtatcttcc acatcgtaca atccggctac
     4921 gtttttagcc gttccgccta cttcggttat ttcaccgaaa acaaaatcca ggtaatccag
     4981 ttgatgcgaa gccagatcgt agaaataccc ggctcccgca acctctttct tcacccgcca
     5041 gggaagtgtt tcccggctta aatcggatgg atacggcggt ataatgaacc ggattttcac
     5101 ttcgcggacc tctcctatct ctccactttc cagcaactgc cttatacgca gaaaataggg
     5161 caacgtcctg cggtaatacg ccacaaaaca ggggactccc gtctcttttg cagcttccgc
     5221 catgatcaca cactcttcga acgaagcagc catgggtttc tccacataca ccggttttcc
     5281 ggctttcatg gccgcaacag catattcggc atgtgatccg ggtggcgtag caacgtatac
     5341 cgcattcaca gcggagtcat taatcaacgc atccgcatcg gaataccact tggctatatg
     5401 gtgccgcaac gcgtaatcct tggctgattc tgcatcacgg cgcatcaccg caactacatc
     5461 cgacccatcg actttgcgga aagccgggcc gcttttcttt tccgttacat ttccgcaacc
     5521 gataaaaccc cagttaatca tattttaata gaagttagag gttgaccctt ggctcttgaa
     5581 tttcttatat cctctgtaag aaacaagcgc cagaacaata actccgaaca atatcgcata
     5641 caggtataca aagacctccg aaaccccttc gttttctccg taggaatgta aaccgctcaa
     5701 gaggtaattt actccccaaa atgtcatcag caccgaagca aatgccatta ccgatgccag
     5761 gttaaagaac caatcgttat tcaatttcct gaccagatgg atatgggtga ccacgctata
     5821 gacaacaacg gtaataagtg cccaggtctc tttaggatcc cagctccagt agcggcccca
     5881 cgattcgtta gcccaaatac cgcccaaaaa agtaccgata gtcatcaggg ccaaccccac
     5941 caacaatgac atgttgttga taatactcag ttccctgacg tgaagcattg ccgtctcttt
     6001 tttagccaca cttagaataa ggaggttaga taaccccaac aacaggctta tcccaaaaaa
     6061 accgtaagca gccaccgtta cagcaacgtg gaacataagc cacggagatt taagtacggg
     6121 tacaagcgtt gtaatctggg gttccatcca gttcagaccg gaaacaaaaa gaattattcc
     6181 tccgaagatg gttgccgttg ccagggtgag atcacttttc cgtccgaaaa tcaatccggc
     6241 cagtatggta gcccaggcta catataccat ggtttcgtac gagttgctcc agggtgcgta
     6301 tcccgatata taccaacgca tagccatgcc gtagacgtga tacagaaaaa ccagaaaaat
     6361 accccagacg agtaccttgg agagaatatt tgtccacatt gcttctttga aaagccgtaa
     6421 aaaagccgtc ataagcaaca ggaggcccag ggcaaaataa ccggtgcggg ttttactgaa
     6481 aatattttgt ttgttgtaac ggatctccgt gcgtattttc ttggggttta tgtgaccggc
     6541 ttgatctgcc ttttgttgaa aggcagctat tgaatcaaga attgtgttgg gttgctgcca
     6601 gtctccgctt tcaagcgacc ggtttacttc cgaaagatac aggggcaaag agcgggaaat
     6661 aaacaaggag tcttcttcag gtattcccga caaatcatct cccggagcat accaggtatc
     6721 gttaggagca tctgcttttg gaaaaagtgt cagcagttta tggttcagca acaagaaaag
     6781 aatgtttatc ttttcatcga gtttgataat gtctttatca aactcatttc tttgcgattc
     6841 gggtttatga taggcttcat ttacccgggc aagaagtttg tagtttccct tcgggtcaaa
     6901 gacctgggca tacgcaaaat gctgttccgg taactgaaac atttttgcca cctcttcgtt
     6961 ggagttggca atgaacggca cctgcatcca catctgtggc atcgtgagta tccccagcaa
     7021 gaactgatcg gaatttagtc ttccaatttt attgttctta tgtatctttc tcaatagctc
     7081 cgctgaaaac gtattcatcg gtataatcct gcctgcgaat tgaaccggta actgtccgaa
     7141 ctccgcagca tgttccaccg ggacagcatt tctttgcacg gcctgaaaaa cagcctggct
     7201 ggtaaagtcc tgtgcgccgg cgctaaaggt tacaagcaaa aaaacggccg tcagctttgc
     7261 cgaacgcctc aattcgttta actggcggct aagcttattt aaccgggagt ttggagtaac
     7321 gaacatcaaa acaaacccgg ctaccaggaa aaaatatccg gcgtaagtaa tgtttcgtcc
     7381 cgctacatcc ttgttgacgg ataaaacagt gccttgctcg tcctcgtcgt aagaggcctg
     7441 aaaaaaacga tatcctttca aatcgagtac attgttcatg taaacacgtg cctcacgcac
     7501 ttcaccatcg atatgtatcc gtaaatcgct ctcataggag gaagggctca tggaaccggg
     7561 atacctgatc aacctgaatt gggtaagctc cagcgtgaac gggagttctt ccttaaacac
     7621 ccctttcgac gtgtgcataa ccattacatt gtttgtttcc ccttcacgga tgtgcattgt
     7681 tccttctttt ccaaagacat gtgttgttaa agccccggcg agaataacaa caaacgcaaa
     7741 gtggataacc gtaagtgccc aacggcgtct ttttataaaa cggtgatcaa taaacgttaa
     7801 aatgaaattt acaaccaaca ggaattggag gaaaaagaaa acgggtgaat aataaatcat
     7861 cactttggcc gcttcggtag tcatctcttt ttcgataaac gtagcaagcg ccattaaaaa
     7921 ggcataaaca agtaaaagaa ctattgttgt tttataggat gcgagaagtt gtcttatttt
     7981 ttccatagtt aattgatatt gaaacagttg tccccgtatt tcaacttaat atcgtttcta
     8041 tacggtacag tctgtgacaa aaatataaaa ataaccggtt gatgcgttat attatctgat
     8101 aaaaatcccc gaaacagcgg tgttccgggg attttgagat caagccaaag ctcgtattaa
     8161 cttacttgtt ctttttcagg cctctttcca ttgttttctc aacaagtcga gcgcagccgg
     8221 gacaactacc tt
//
LOCUS       DAOSDR010000087         8198 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrimonas sp. isolate AMR_MDS_1826
            NODE_3253_length_8198_cov_3.893651, whole genome shotgun sequence.
ACCESSION   DAOSDR010000087 DAOSDR010000000
VERSION     DAOSDR010000087.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863355
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrimonas sp. (wastewater metagenome)
  ORGANISM  Petrimonas sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Dysgonomonadaceae; Petrimonas.
REFERENCE   1  (bases 1 to 8198)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8198)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:10:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,720
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 35
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8198
                     /organism="Petrimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3253_length_8198_cov_3.893651"
                     /isolate="AMR_MDS_1826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2023866"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            56..1438
                     /locus_tag="PLI30_04900"
     CDS             56..1438
                     /locus_tag="PLI30_04900"
                     /inference="COORDINATES: protein motif:HMM:NF014904.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[Fe-Fe] hydrogenase large subunit C-terminal
                     domain-containing protein"
                     /protein_id="HOI78927.1"
                     /translation="MKDRDYTHAIQIDTDKCIGCSHCMQVCPTQAIRIVEGHAGIMSE
                     RCVDCGECYRVCPVRAIYVKDDGLSVIRHDAYRVALVPSVFIGQFPSEHTASEIMEAV
                     RLIGFNEVLEVEQAVDFMKKEYARFAGEAENVVRPVISSFCPAIVRLIQVHYPSLTGQ
                     ILKIKAPHDIAALYVRKSKEKAGIDPRNIFICYVTPCAAKIVAARAPVGEKVSAIDGA
                     IDMKEVYNLVSKTLMEKKEARLSKQFANMSPDSVNWSLSGTEKQYFRGRALAIDGMDN
                     AMEFLDRLESGRVTGVDFLEMRACDQGCAGGILCPGNRFLTVERLEQREKKLVHLTEV
                     NKPGKNDLMDYAEELHQVSTTDPVYPRDGLLLDEDMEKALQKMDRIKKLNSYLPGFDC
                     GACGAPTCRSLAEDIVKEKATISYCVFVQRVMEKNYNLSPDQAFHVIEKIWGKDRLKK
                     YQLQNGKTES"
     gene            1419..1769
                     /locus_tag="PLI30_04905"
     CDS             1419..1769
                     /locus_tag="PLI30_04905"
                     /inference="COORDINATES: protein motif:HMM:NF018753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DRTGG domain-containing protein"
                     /protein_id="HOI78928.1"
                     /translation="MEKPNLNKLTKELDFECLSGKTLLDRIPQSAYVSDLLSDVMGKA
                     RAGMIWVTSQVHKNIVAVASLKELSAIVIVNERPVEKELLEQAENEEVVVLASNLPAF
                     ETVGKLYNYLAGRC"
     gene            1799..2512
                     /locus_tag="PLI30_04910"
     CDS             1799..2512
                     /locus_tag="PLI30_04910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019541393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HOI78929.1"
                     /translation="MKHFKADLHIHTCLSPCGDLEMSPGSIVQTAREKGLDIIAVTDH
                     NSTRNVRPCLEIGKEFSLFVIPGCEVNTQEEVHCLCYFPDLKALDRFQQYLDEKIPDI
                     KNNPELFGYQVAVDKDNVIVYEEKRSLYTGIRDDIEGVQQKVHSLGGVFVPAHIDRMK
                     NGIYGQLGFIPVDLHYDALEISKRIHPEDFLRLHPELVAKKILRSSDAHFLSHIASVY
                     TDFYLERPDWESFKKAFYG"
     gene            2505..3071
                     /locus_tag="PLI30_04915"
     CDS             2505..3071
                     /locus_tag="PLI30_04915"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOI78930.1"
                     /translation="MVDLAMHMMDIVQNAVRANATKIDIGFLEYSRDATLTFSVNDNG
                     SGMTPETVEKLEDPFFTTRTTRRVGLGVPFLKMSSEQAGGSLKVVSSPGVGTRIEAVY
                     RTDNPDCLPLGDLAGYLVLLLVANPGIRFSFRYKMDENEFSLDTGEWTEQGITEFSKN
                     EMAPAVKEYIHENLKELYKNRNTESYLC"
     gene            3096..3497
                     /locus_tag="PLI30_04920"
     CDS             3096..3497
                     /locus_tag="PLI30_04920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009135593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S) ferredoxin domain-containing protein"
                     /protein_id="HOI78931.1"
                     /translation="MAGIKTLADLKRKQEEVKTQIDLRDKAENPGSQVQVKVAMATCG
                     IASGAKNVMDFFVRELPKRGIGAVVTQTGCMGYCFAEPTVEITIPGKDPIVFGYVDPA
                     KADEIIERYIKQGELVDGIIPQNYNTITNTD"
     gene            3514..5352
                     /locus_tag="PLI30_04925"
     CDS             3514..5352
                     /locus_tag="PLI30_04925"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019541396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HOI78932.1"
                     /translation="MSQYKIHILVCAGTGCQSSQSGLIYEMLQDELDWKGLKDEVQVI
                     KTGCFGFCEKGPIVKILPDNTFYTQVKPEDIREIIDEHIIKGRSVHRLLYLDPETEEH
                     ILDSKHMGFYKKQLRIALRNCGTIDPENIDESIARDAYQALGRALTELTPQETIDIIK
                     RSGLRGRGGAGFPTGIKWEIASRNDADQKYVVCNADEGDPGAFMDRSILEGDPHSVLE
                     AMAICGYCIGATRGLIYIRAEYPLAIKRLKIAIKQAREYGLLGENIFGTGFSFDINLR
                     YGAGAFVCGEETSLIHSMEGKRGEPSNKPPFPAQKGYLGKPTNVNNVETFANIPVILL
                     KGAEWFSSIGTEKSKGTKVFALAGKINNVGLIEVPMGITLREIIYEIGGGIKGGKKFK
                     AVQTGGPSGGCLTEKHLDTPIDYESLTAAGSMMGSGGMIVLDEDDCMVSVAKFYLDFI
                     VEESCGKCAPCRIGNKRLYETLERICDGKGTGEDLEKLENLSSVIKDTSLCGLGQTSP
                     NPVLSTLNNFQEEYREHVFDKKCRSGKCRNLLYYVIDDENCVGCMACVRSCPVNAITG
                     AKKQTHFIHQDKCIKCGACMEKCQFDAISLKSDYRIGTSVPDTADI"
     gene            5372..7147
                     /locus_tag="PLI30_04930"
     CDS             5372..7147
                     /locus_tag="PLI30_04930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019541397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent [FeFe] hydrogenase, group A6"
                     /protein_id="HOI78933.1"
                     /translation="MIELTIDNKRITVEPGTTILKAARQAGIEIPTLCHFEMCDMGIE
                     NKPGGCRICVVEVEGRRNLAPSCVTEAQNGMVINTHNIRVLNARKTVLKLIISDHPFD
                     CLVCAKSGQCELQHLATQFGIRSMPYGGEQSHYRKDTSPSIIRDVDKCILCRRCEMVC
                     NEVQTVGALSAVNRGFMAVVAPAFEMNLEKSPCTYCGQCVSVCPTGALTEVDHSNEVL
                     RALSNPKKTVVVQTAPAVRAALGEEFGLEPGTLVTGKLVAALRRLGFDYVFDTDFSAD
                     LTIMEEGTELLNRLKGHLAGDPNVRIPLLTSCCPGWVNFFEHNFPDLLDVPSTAKSPQ
                     QMFGAIAKSYFAEKIGVSREDLIVVSIMPCLAKKYECQRDEFKVDGNPDVDYSISTRE
                     LAAFIKQANIDFLGLPDEEFDDPLGESSGAGVIFGNTGGVIEAACRTAYELYTGKTLD
                     KVEFHELRGMEGIRSATVDFDGLKLNIGIAHGLGNARKLLDEVRAGKSQYHAIEIMAC
                     PGGCISGGGQPYTHLKEEILVKRTAALYTEDQRKAIRKSHENPSIIKLYNEFLGEPGS
                     HRAHSLLHTEYFPKSNEVTISLNNH"
     gene            7199..7702
                     /locus_tag="PLI30_04935"
     CDS             7199..7702
                     /locus_tag="PLI30_04935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019541398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HOI78934.1"
                     /translation="MPEERDVLKVKVNLPQEKIDIINRICESFDNDPGELINVLHEAQ
                     HEFGYLPAEVQDVVAHNLKVSVAHVYGVVTFYTYFTMIPKGEFPISVCTGTACYVRGA
                     EKVLEEFKKELNIKVGETTPDGKFSINCLRCVGACGLAPVVLVGERVYGRVSPDGVKN
                     ILDEYRK"
     gene            complement(7704..>8198)
                     /locus_tag="PLI30_04940"
     CDS             complement(7704..>8198)
                     /locus_tag="PLI30_04940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013816157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent transferase"
                     /protein_id="HOI78935.1"
                     /translation="RLVGIKDMTGASLSPFDAFLIARGLKTLEIRMEKHCENAQKVAE
                     FLEKHAAVETVLFPGLASFPQAELAKKQMSLPGAIISFEVKGGIEAGKKLLKRLQLLT
                     ISVSLGDAETLIQHPASMTHSAYSPEERLAGDISDGLIRLSVGLENATDIIADLKQGL
                     DGLR"
BASE COUNT         2219 a         1848 c         2204 g         1927 t
ORIGIN      
        1 tttgaaatag atatcagaca gcaaagcggt ctgcaatctg aagtcaatga aaaatatgaa
       61 ggaccgggat tacacacacg ccattcagat cgataccgac aaatgtatcg gttgctcaca
      121 ctgtatgcag gtttgtccta cccaggccat ccgtatcgta gaaggacatg ccggcattat
      181 gtctgaacgt tgtgtggatt gcggcgaatg ctatcgtgtc tgccctgtaa gggcgattta
      241 tgttaaggat gatggcttgt cggttataag gcacgacgct taccgtgttg cgctggttcc
      301 ttcggttttt atcggccagt tcccgtcgga acacacggct tccgagatta tggaagcggt
      361 cagactaatt ggcttcaatg aggtactgga ggtggagcag gcagtggatt ttatgaaaaa
      421 agaatacgcg aggtttgccg gcgaagcgga gaatgtagtc cggccggtca tcagttcgtt
      481 ttgtcctgcg attgtcaggc ttattcaggt tcattacccg tcgcttaccg ggcaaatact
      541 gaaaataaaa gcacctcacg acatagccgc gctctatgtt cgcaagtcaa aagaaaaagc
      601 cggaatagat ccgcggaaca tttttatctg ctacgtgacg ccatgtgctg cgaaaattgt
      661 cgctgcaagg gctccggtag gcgaaaaagt ttccgctatt gacggtgcca tagatatgaa
      721 agaggtatac aacctggttt caaaaacgtt gatggaaaaa aaagaggcta ggttgtcgaa
      781 acagtttgcc aacatgagtc ccgactccgt gaactggtct ttgtcgggaa ccgagaagca
      841 atacttccgg ggccgtgccc tggctattga cggaatggac aacgccatgg aattcttaga
      901 caggctggag tcgggcaggg ttaccggagt ggattttctt gaaatgcgcg cttgcgatca
      961 gggttgtgca ggtggtattc tttgtcccgg caaccgtttc ctgacggttg aacgattgga
     1021 acagcgggag aagaagctgg ttcacttgac ggaagtgaat aaaccgggca agaacgacct
     1081 gatggattac gccgaagaat tgcatcaggt gtctacgacc gacccggttt atccgcgcga
     1141 cggactgctg ctcgatgaag atatggaaaa ggcacttcag aaaatggatc gcatcaagaa
     1201 gcttaactcc tatcttcccg gattcgattg tggtgcctgc ggtgcaccca cctgtcgtag
     1261 cctggcggaa gacatcgtta aggaaaaagc gaccatttcg tactgcgttt ttgtacagcg
     1321 cgtgatggaa aaaaattata acctgagtcc cgatcaagct tttcacgtca tcgaaaagat
     1381 atggggcaag gatcggctca aaaaatatca acttcagaat ggaaaaaccg aatcttaata
     1441 aactgacaaa ggagttggat ttcgaatgtt tgtccggcaa aacgttgctg gacagaattc
     1501 cccaatcggc ttatgtttcc gatttgctga gcgatgtgat gggaaaagcc cgggccggaa
     1561 tgatttgggt gacctcgcaa gttcacaaga atatagtagc ggtggcctcg ctcaaggagt
     1621 tgagtgcgat tgttatcgtg aatgagcgtc cagtggaaaa agaattgctg gagcaggcgg
     1681 aaaatgaaga ggtggtagtg ctggcatcta acctgccggc atttgaaaca gtcgggaaat
     1741 tatataatta tttagccggc agatgttagg ccgctgcgct gacagagtcc agaccgcaat
     1801 gaaacatttc aaggccgacc tgcacattca cacttgcctc tctccctgtg gagacttgga
     1861 gatgagtccc ggcagtattg tgcagactgc ccgggagaaa gggttggaca ttattgccgt
     1921 gaccgatcat aattccaccc ggaacgtgag gccttgtctc gaaataggga aagagttcag
     1981 cctgtttgtt atccccgggt gcgaagtaaa tacgcaggaa gaggtgcatt gcttgtgcta
     2041 ctttcccgac ctgaaagcac tagacagatt ccaacagtat ttggacgaga aaatcccgga
     2101 catcaagaat aatcctgaac tttttggtta ccaggtagca gtggataagg acaatgtgat
     2161 tgtatacgaa gagaagaggt cgctctatac gggaattcgg gacgatattg aaggtgtgca
     2221 gcaaaaagta cattcactgg gaggtgtttt tgtcccggca cacatcgatc gcatgaaaaa
     2281 tggcatttac ggccagctgg gatttattcc tgtcgatttg cattatgatg ccttggagat
     2341 ttctaaaagg atacatcccg aagattttct gagactgcat cccgagcttg tagcgaagaa
     2401 aatcctgcga agttccgatg cgcactttct gagccatatt gccagcgtgt ataccgattt
     2461 ctacctggaa aggccggact gggaaagttt taagaaagcg ttttatggtt gacctggcca
     2521 tgcatatgat ggatatcgtc cagaacgctg ttcgggcaaa cgcgacaaag atcgatatcg
     2581 gattcctgga atacagtcgg gatgccacac ttacgttttc ggtaaatgac aacggttcgg
     2641 ggatgacccc cgaaacggta gagaagctgg aggatccgtt ctttaccacg cgaaccactc
     2701 gaagggtagg cctgggagtg ccgttcctga aaatgtcgag cgaacaggca ggaggctctt
     2761 tgaaagtagt atcgagccca ggggtgggaa cccggattga agcggtttac agaaccgata
     2821 atcccgattg cctcccgctg ggtgacctgg ccggatacct ggtgttgttg ctggtcgcca
     2881 atcccggtat tcgtttcagt ttccggtaca agatggacga aaatgaattt tcacttgata
     2941 ccggggaatg gaccgagcag gggatcacgg aattttcgaa aaacgaaatg gctccggctg
     3001 taaaagaata tattcatgaa aacctgaaag agttatacaa gaacagaaac acggagagtt
     3061 acttatgttg aataatttaa tttaaactta ctattatggc aggaattaaa acattggctg
     3121 acctgaaacg caaacaggag gaagtcaaaa cccagatcga cctgagggat aaggccgaaa
     3181 atcccgggtc gcaagtgcag gtgaaagtgg cgatggccac ctgcggtatt gcgtcggggg
     3241 ccaaaaatgt gatggatttt ttcgtgcgcg aattgcctaa acgcggcata ggagccgttg
     3301 tgacgcaaac cggctgcatg ggttattgct tcgcggagcc taccgtggag atcactattc
     3361 ccggaaaaga cccgatcgta tttgggtatg tcgaccccgc taaagcggat gagatcatcg
     3421 aacgttatat caaacaaggt gagttggtag acggaattat accgcagaac tacaacacca
     3481 taacaaacac agattaaccg aaaataatta cctatgagtc agtacaaaat acacatactc
     3541 gtttgtgcag gaacaggttg tcagtcgtca caaagtggac tgatctacga aatgctgcag
     3601 gatgaactgg actggaaagg gttgaaagat gaggttcagg tcattaaaac cggatgtttt
     3661 gggttttgtg agaaaggacc cattgtgaag atacttcccg ataatacctt ttatacacag
     3721 gtaaaacccg aagatatccg tgaaattatt gatgagcaca ttattaaagg acgcagcgtc
     3781 caccggttgc tctatctgga tccggaaacc gaagaacata ttctggattc aaaacacatg
     3841 gggttttata aaaaacagct tcgtatcgca ctgcggaatt gcggtaccat tgacccggaa
     3901 aatatagacg aatccattgc acgcgatgct tatcaagctc ttgggagggc gttgaccgaa
     3961 cttaccccgc aggaaactat tgatatcatt aaaagatcag ggctacgcgg gcggggaggt
     4021 gccggtttcc cgaccggcat aaaatgggaa attgccagca ggaacgatgc agaccagaaa
     4081 tacgtggtgt gtaatgctga cgaaggcgat cccggtgctt ttatggaccg ttcgatcctc
     4141 gagggtgacc ctcacagtgt tcttgaagct atggccatct gcggatattg tatcggtgca
     4201 acaagaggac tgatctatat ccgggcggaa tatccgttgg ctatcaagcg cctgaagatt
     4261 gcgataaaac aagcacgtga atacggcttg ttgggtgaaa atattttcgg aaccggcttc
     4321 agtttcgata taaaccttcg ctacggcgca ggtgcatttg tttgtgggga agaaacctca
     4381 ctcatccact cgatggaagg gaaacgcggg gaaccatcca ataagcctcc attcccagcg
     4441 caaaaaggat atttgggaaa acctaccaac gtgaacaacg tggagacgtt tgccaatatt
     4501 ccggttatcc tgttaaaagg agccgaatgg ttttcttcca tcggaaccga aaagtcgaaa
     4561 ggaacaaaag tgtttgccct tgccggaaag atcaataacg tcggactgat tgaagtgccg
     4621 atgggaatta cgctccgtga aattatttac gagattggcg gaggcattaa aggcggtaaa
     4681 aaattcaaag ccgtgcagac cggggggccc tcaggcggtt gcttgacgga aaaacacctg
     4741 gatacgccca tcgattacga aagccttacg gctgccggtt ccatgatggg gtcgggtggc
     4801 atgatcgtgc tcgacgaaga cgattgcatg gtttccgttg cgaaattcta cctcgacttt
     4861 attgtggaag agtcgtgcgg aaagtgcgct ccctgccgta tcggtaacaa acgcttatac
     4921 gaaacgctgg agcggatctg cgacggaaaa ggaaccgggg aagatttgga aaaactcgaa
     4981 aacctttcca gcgtgattaa agacacttcg ttgtgcgggc tgggacaaac atcccccaat
     5041 ccggtactgt cgacgctgaa taattttcag gaagagtaca gagaacacgt cttcgataaa
     5101 aaatgccggt cgggcaaatg caggaacctg ctttattatg tcattgatga tgagaactgt
     5161 gtcggctgca tggcttgtgt gcggtcttgc cccgttaatg ccatcaccgg agccaagaaa
     5221 cagacccact ttattcatca ggataaatgc atcaagtgcg gtgcctgtat ggagaaatgc
     5281 caattcgatg ccataagcct gaaatcggat tacaggattg gcacttcggt gcccgacact
     5341 gccgacatct aacacctaaa aaactaaaaa tatgatagaa ctcaccatag acaacaaaag
     5401 aataacggtg gaaccgggaa ccaccatttt aaaagctgcc cggcaagcag gtatcgaaat
     5461 tccgacgctg tgccactttg aaatgtgcga catgggtata gaaaacaaac ccgggggatg
     5521 ccgcatatgt gtggtggaag tggaaggacg caggaacctt gctccatcgt gtgtgaccga
     5581 ggcgcagaac ggaatggtca taaacacgca caacatccgc gtgctgaacg cacgaaaaac
     5641 cgtgttgaag ctgatcatct cagaccatcc cttcgattgt ctcgtgtgtg cgaaatcggg
     5701 acaatgcgaa ctgcagcacc tggctacaca gtttggcatc cggagcatgc cctacggcgg
     5761 cgaacagtcg cactacagaa aagatacctc cccttccatc attcgcgatg tggataaatg
     5821 tatattgtgc cgccgctgcg aaatggtttg taacgaagtg caaaccgtcg gtgccctttc
     5881 ggcggtcaat cgcggattta tggcagtggt ggctcctgct ttcgaaatga acctcgaaaa
     5941 gtctccctgt acctattgcg ggcagtgtgt ctcggtttgc ccgacgggag cgcttaccga
     6001 ggtagaccac agcaacgagg tgcttcgtgc gttatcgaat ccgaagaaaa cggtggtggt
     6061 gcaaaccgca ccggccgtac gcgcagcgct gggagaagaa ttcggactgg agccgggaac
     6121 gctggtcacc gggaagctgg tggcggcact tcgccggctc ggtttcgatt acgttttcga
     6181 taccgatttc agtgccgacc tcaccatcat ggaagaggga acggaattgc tgaaccgcct
     6241 caaaggacat ttggccggag atcccaacgt ccggattccg ttgctgacat cctgctgtcc
     6301 gggttgggtg aatttctttg aacacaattt tcccgatctg ttggatgtac catcaacggc
     6361 aaagtcgccg cagcaaatgt ttggtgcaat tgctaaatcc tattttgccg aaaagatcgg
     6421 tgtgagccgt gaagacctga tcgtggtttc cattatgcct tgcctggcca agaagtacga
     6481 atgccagcgc gacgaattca aggtggacgg caatcccgat gtggattatt ccatttccac
     6541 gcgtgaactg gctgctttca tcaagcaggc aaacatcgac ttcctggggt tgcccgatga
     6601 ggagttcgac gaccctctcg gcgaatcgtc gggtgcaggt gttattttcg gaaacaccgg
     6661 aggcgttatc gaggccgcct gtcgcacggc ttacgaactc tacacgggca aaaccctcga
     6721 taaggtggag tttcatgaat tgcgcggtat ggaaggtatt cgttccgcaa cagtggattt
     6781 cgacggattg aaactgaata tcggaatcgc gcacggactg ggtaatgccc gtaagttact
     6841 ggacgaggtc cgtgccggaa aatcgcagta tcatgccatt gaaatcatgg cttgccccgg
     6901 aggatgcatc agtggaggtg gacagcctta cacacacctg aaggaagaga tactggtgaa
     6961 acgcaccgca gccctgtata cggaagatca acgcaaggcc atccgtaaat cgcatgagaa
     7021 cccatcgatc atcaagctct ataacgagtt tttgggcgag ccgggtagcc acagggccca
     7081 cagcctgctc catacggaat attttccgaa gagtaatgag gtaaccattt cgttgaacaa
     7141 tcattaaaga aagtatatga gccagaaaac agatatagca ccggcagtat ttaaatacat
     7201 gcctgaagag agagacgtgt tgaaagtaaa ggtaaacctg cctcaagaga agatagacat
     7261 cattaaccgg atctgtgaat cgttcgataa tgatcctggc gagctgatca acgttttgca
     7321 cgaagcacag cacgaattcg gatacctgcc ggcagaagtc caggatgtgg ttgcgcataa
     7381 cctgaaagtt tccgtggcac acgtgtatgg tgtggtaacc ttctatacct atttcaccat
     7441 gattcccaag ggcgagtttc ctatctccgt ttgtacggga acagcctgtt atgtgcgtgg
     7501 tgcggaaaag gtgctcgaag aatttaagaa agaactgaac ataaaggtgg gcgaaaccac
     7561 accggatggc aagttctcca tcaattgcct gcgttgtgtg ggcgcgtgcg ggctagcacc
     7621 cgtagtgctg gttggcgaac gggtttacgg acgtgtttca cccgacgggg taaagaatat
     7681 tttggacgag tacaggaaat aaactaccgt aacccatcca gtccctgctt taaatcggca
     7741 atgatgtctg tggcattttc cagccctaca gacagacgga taagcccgtc gctgatgtcg
     7801 ccggccagcc tctcttcggg tgaatacgcg gagtgagtca tgctggccgg atgctgaatg
     7861 agggtttcgg catcccccag gcttactgaa atggtcaaaa gctgcaatct tttaagcagt
     7921 tttttgccgg cctcgatccc tccttttact tcaaaagaga tgatggcccc cggtaacgac
     7981 atctgctttt ttgccagttc agcctgcgga aacgatgcta atcctggaaa caggaccgtt
     8041 tcaacggctg cgtgcttttc caggaattct gccacctttt gtgcgttctc gcagtgtttc
     8101 tccatccgta tctcaagtgt ttttagcccg cgtgcaatca gaaaggcatc gaaagggctc
     8161 aggctggctc ccgtcatgtc ctttataccg acaagccg
//
LOCUS       DAOSDR010000088         8192 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrimonas sp. isolate AMR_MDS_1826
            NODE_3258_length_8192_cov_4.317930, whole genome shotgun sequence.
ACCESSION   DAOSDR010000088 DAOSDR010000000
VERSION     DAOSDR010000088.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863355
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrimonas sp. (wastewater metagenome)
  ORGANISM  Petrimonas sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Dysgonomonadaceae; Petrimonas.
REFERENCE   1  (bases 1 to 8192)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8192)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:10:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,720
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 35
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8192
                     /organism="Petrimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3258_length_8192_cov_4.317930"
                     /isolate="AMR_MDS_1826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2023866"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..992)
                     /locus_tag="PLI30_04945"
     CDS             complement(<1..992)
                     /locus_tag="PLI30_04945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002561100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium ion-translocating decarboxylase subunit
                     beta"
                     /protein_id="HOI78936.1"
                     /translation="MNTLLTLGDNLKTFWDYTGMANASTGHIIMILVGLIFIYLAIAK
                     EYEPLLLIPIGFGILIGNIPFNEAAGLQVGIYEEGSVLNILYQGVVKGWYPPLIFMGI
                     GAMTDFSSLIANPKLFLIGAACQFGIFGAYAAALLWGFAPNEAGAIGIIGGADGPTAI
                     FLTSKLAPQLLGAVAISAYSYMALVPVIQPPFMRWLTTPKERMIKMKSPRVVSQTEKI
                     LFPIVGLLLTTFLVPSGLPLLGMLFFGNLLKESGVTRRLAETARGPLIDTITILLGLT
                     VGASTQATTFLRPESVKIFVIGAFSFVVATCAGILFVKFFNLFLKKDNKINPLIG"
     gene            complement(1004..>1204)
                     /locus_tag="PLI30_04950"
     CDS             complement(1004..>1204)
                     /locus_tag="PLI30_04950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004304406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein subunit"
                     /protein_id="HOI78937.1"
                     /translation="QSPLPGIILDIRCQVGDTVKKGQTIMILEAMKMENNILSNANGK
                     ITGILVNKGDSVLEGAELVTIE"
     assembly_gap    1205..1304
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1305..1596)
                     /locus_tag="PLI30_04955"
     CDS             complement(<1305..1596)
                     /locus_tag="PLI30_04955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein subunit"
                     /protein_id="HOI78938.1"
                     /translation="MKEFKYKINGAPYRVIINSSDSSVVELEVNGTPYKVELEKKESK
                     PLSKIKKQAITTPTPQTTSTPLVTRPKEATGKNTIQSPLPGIILDIRCQVGDT"
     gene            complement(1633..2538)
                     /locus_tag="PLI30_04960"
     CDS             complement(1633..2538)
                     /locus_tag="PLI30_04960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009018780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008948 - oxaloacetate decarboxylase
                     activity [Evidence IEA];
                     GO_function: GO:0015081 - sodium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0036376 - sodium ion export across plasma
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OadG family transporter subunit"
                     /protein_id="HOI78939.1"
                     /translation="MNNLKQLLFLSALILLVSCTIKDENPRWVINEVMVDNQTGYMDD
                     FGQRNGWIEIFNNTYKTQDLGGRYLTDDPNNPKKYPIPRGDVLTKIPPRQHALFWADN
                     EPFNGTFHVNFKLDPNKDNYIALYENDGKTLLDEIIIPAGLPTDKTYGYPADGIKYDQ
                     NGNTLGTQLERITPSSNNAVLEENPKVVSLQENDPFGVTITITSMLVVFLGLILLYLI
                     FKAIGNTAKNISHKRVASAGTLSAVRGESQLTGEVLAAISAAIHELNQDVHDIESTIL
                     TITEVRRKYSPWSSKLYTLRQDPRR"
     gene            complement(2550..4112)
                     /locus_tag="PLI30_04965"
     CDS             complement(2550..4112)
                     /locus_tag="PLI30_04965"
                     /EC_number="6.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009128927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA carboxylase subunit beta"
                     /protein_id="HOI78940.1"
                     /translation="MSIQLEKVKELIQLREKARLGGGEKRIESQHLKGKYTARERLAM
                     LLDEGSFEEFDMFVEHRCHNFGMEKTKFLGDGVVTGYGTIQGRLVYVFAQDFTVSGGS
                     LSEMQSQKICKVMDQAMKMGAPLIGINDSGGARIQEGINALAGYAEIFQRNILASGVI
                     PQISGIFGPCAGGAVYSPALTDFNIMTQGTSYMFLTGPKVVKTVTGEDVTQEQLGGAS
                     VHTSKSGVAHFSAQTEEDAIQMIQKLLSYIPQNNLEEPPVFKSSDPIDRLEDGLNEII
                     PDSANKPYDMYEIIGAIVDSGEFLEVHADYAKNIIVGFARFNGQSVGIVANQPKFLAG
                     VLDINASRKAARFVRFCDAFNIPIVSLVDVPGFLPGTGQEYGGVITHGAKLLYAYGEA
                     TVPKVTVTLRKSYGGAHIVMSCKQLRGDINYAWPSAEIAVMGAEGAVEVLYSKEIAAE
                     KDPEKLALILETKKKEYNDLFTNPYEAARYGYIDDVIEPRNTRFRVIRALQQLQTKKL
                     VNPPKKHDNLPL"
     gene            complement(4195..4599)
                     /gene="mce"
                     /locus_tag="PLI30_04970"
     CDS             complement(4195..4599)
                     /gene="mce"
                     /locus_tag="PLI30_04970"
                     /EC_number="5.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006842412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA epimerase"
                     /protein_id="HOI78941.1"
                     /translation="MELTHIEHIGIAVKNIEEQLPYYEGILGLKCYNIETVEDQKVKT
                     AFFMIGQTKIELLEPTSEESTIAKFIEKRGEGIHHIAFATKNLGESLREIEAKGVRLI
                     DTQPRAGAEGLNIAFLHPKSTGSVLTELCEHP"
     gene            complement(4681..5664)
                     /gene="pfkA"
                     /locus_tag="PLI30_04975"
     CDS             complement(4681..5664)
                     /gene="pfkA"
                     /locus_tag="PLI30_04975"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019540273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HOI78942.1"
                     /translation="MDSNVKSIGVLTSGGDAPGMNAAIGAVTRAAIFNGMRVFGIYRG
                     YKGLISNEIEEFKTNSVSNIIQQGGTILKTARCEEFMTEAGRKTAYENMQRHGIDALV
                     VIGGDGSLTGAGVFANEYNIPIVGLPGTIDNDLNGTDTTIGYDTALNTIMQSMDKIRD
                     TATSHERLFFVEVMGRNCGYLALNSAIASGAEAAIIPEISMEKDQLAELIDQGFRKSK
                     SSSMVLVTESEITGGAMNLAERVKKQYPQYDVRVSILGHLQRGGSPTAQDRILASRMG
                     IAAIQALLENQRNVMIGIRENEIDYVPFKRAIKKDREISPELLEVLEISSI"
     gene            complement(5761..6654)
                     /locus_tag="PLI30_04980"
     CDS             complement(5761..6654)
                     /locus_tag="PLI30_04980"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019540274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA];
                     GO_process: GO:0050992 - dimethylallyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HOI78943.1"
                     /translation="MSKIEIDKQSGCCFGVAKAISKAEEELKKGGTLYCLGDIVHNNI
                     EVERLAKMGLITINHDEFKKLRDVRVLLRAHGEPPSTYQIAKENNIDLIDASCPVVLG
                     LQKRIKKKFITKQNEDGQIVIFGKKGHAEVNGLMGQTDESAIVIEKKEDIDKLDFSRD
                     ISLFSQTTKPLDGFQEIGELIKERMKNGATFEFYDTICRQVSNRVPNMQEFAKNHDLV
                     IFIAGEKSSNGKVLFAECEKYNRNSHLIHHPDQLDTEWIRDDISIGICGATSTPMWQM
                     EVVAKKISELIPEMKVEKAAF"
     gene            complement(6656..7339)
                     /gene="cmk"
                     /locus_tag="PLI30_04985"
     CDS             complement(6656..7339)
                     /gene="cmk"
                     /locus_tag="PLI30_04985"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013064051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - cytidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HOI78944.1"
                     /translation="MGKINIAVDGFSSCGKSTIAKGLARELGYTYIDSGAMYRAVALF
                     AYRKGWITDTEIDDAALQMHISEIDISFKTNSEGQQETYLNGENVEKEIRSLEIGNGA
                     SRVSALGFVRRELVRQQQAMGKKKGVVMDGRDIGTVVFPDAEFKIFLTASPEVRAQRR
                     YEELQAKGNPVTYEDTLANVLERDERDTTRIESPLRKATDAIELDNSHITITEQLQWA
                     MDMFNKITK"
     gene            complement(7420..>8192)
                     /locus_tag="PLI30_04990"
     CDS             complement(7420..>8192)
                     /locus_tag="PLI30_04990"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013646.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HOI78945.1"
                     /translation="FAFTIDRKKFGQWQLQKIKAFPNIEIRISSKATVVNKDFIVVGG
                     EKIGFKYLVGADGSNSIVKTFLGLKSRRKIIGIQYIISTNKYQDFEIFFQPEYFSAWY
                     AWIFPHRSYISVGCGADPKHVSPKKLKENFHQWLNDRQIDISKGKYEAFPMDADYNGI
                     RFDNIFLTGDAAGLVSPFIGEGIYQALISGEETAKTILNPSYISDKMPAVIHKHKRHQ
                     QLINLMIKSGRLKSLFFATGQQLFKIPKYEKKAIELFG"
BASE COUNT         2017 a         1801 c         1740 g         2534 t
ORIGIN      
        1 ccgataagcg gattgatctt gttgtctttt ttcaggaata aattgaaaaa tttaacaaac
       61 agaattcccg cacaagtggc taccacgaaa gaaaaagcgc caataacaaa aatcttcacc
      121 gattcgggac gcaggaaagt ggtggcttgt gtggatgccc ccaccgttaa tcccaacaag
      181 atggtgatag tgtcgatcag cggaccccgg gccgtttcag ccaaacggcg tgttactccc
      241 gattctttca gcaggttgcc gaaaaagagc atacccaata agggaagtcc ggaaggcacc
      301 aggaaagtag tcaacaatag gccgacaatg gggaacaaga ttttttcggt ttgagaaact
      361 acacgaggag acttcatttt aatcatacgt tcttttggtg tagttaacca tctcataaac
      421 ggcggttgaa tcaccggaac caacgccatg taagagtatg ccgatatagc tactgcaccc
      481 agtagttgcg gagccagctt cgacgtaagg aaaatagccg tcgggccgtc tgccccacca
      541 ataataccga tagctccagc ttcgttaggg gcaaatcccc acaataacgc tgctgcgtat
      601 gctccaaaaa tcccaaactg acaggcagct cctatgagaa agagcttcgg gtttgcaatc
      661 aacgacgaga agtcggtcat ggctccaatc cccataaaaa taagcggggg ataccatcct
      721 tttacaacgc cctgatagag gatgttaagt actgaaccct cttcatagat ccctacttgc
      781 aagccggctg cttcattgaa cgggatgttc ccgataagaa taccaaaacc gatgggaatt
      841 aacagcaacg gctcgtattc ttttgcaatg gccagatata taaagattaa gccaaccaga
      901 atcatgatga tgtgcccggt actggcatta gccattccgg tatagtccca aaatgttttc
      961 aggttgtcac ccaaagtcaa aagtgtattc attttttcct tttttattct atggttacta
     1021 attcggcccc ttcaagtaca gagtctcctt tgttaaccaa gatccctgtt atctttccgt
     1081 tggcgtttga caagatgttg ttttccattt tcatagcttc caggatcatg atggtttgtc
     1141 ctttctttac cgtatcacct acctggcagc gaatatcgag aataattccc ggaagcggag
     1201 actgnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1261 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnccgtat cacctacctg
     1321 gcagcgaata tcgagaataa ttcccggaag cggagactgg atcgtatttt ttccggttgc
     1381 ttctttcggc cttgtgacaa gcggagtaga ggtcgtttgc ggtgtaggcg tagtaatggc
     1441 ttgttttttg atttttgaaa gcggtttgct ttcctttttt tccaattcta ctttatacgg
     1501 tgttccgttc acttcaagtt caacgacaga actatcggag ctgttgataa tcactcggta
     1561 gggagcaccg ttaattttat atttaaattc tttcataagg aatatttctt tctgagtaaa
     1621 tggattaatg aattatctgc ggggatcttg acgcagggtg tataatttgg aactccaggg
     1681 agagtatttg cgtctgactt cagtgatggt aaggatggta ctttcaatat catgaacgtc
     1741 ctgattcagc tcgtgaattg ctgctgatat agcggctagc acctcgccgg taagctggct
     1801 ttcgcctctc actgccgata aggtaccggc actggcaaca cgtttgtggg aaatgttttt
     1861 cgcagtgttt ccaatcgctt tgaagataag atacagcaag atcagtccca ggaaaaccac
     1921 cagcatcgag gtaatggtta tggttacacc aaagggatcg ttctcctgta gtgaaaccac
     1981 ctttggattt tcttccagaa cggcattgtt gctgcttggg gttattcgtt caagctgagt
     2041 gcccagtgta tttccgtttt gatcgtattt gataccgtcg gccggataac cgtaagtttt
     2101 gtcggtgggt aatcctgcag gaataattat ctcatcgagc agtgttttac cgtcattttc
     2161 atacaatgcg atgtaattgt ctttgttcgg atcaagcttg aaattcacgt ggaaggtccc
     2221 gttgaacggc tcgttgtctg cccagaacag tgcatgctgt cggggtggaa tttttgtgag
     2281 aacatcaccc cgcggaattg gatatttttt cggattgttg ggatcatcgg taagatatct
     2341 tccacccaga tcctgcgttt tatacgtgtt gttgaatatc tcgatccatc cgtttcgttg
     2401 accgaagtcg tccatgtacc ctgtttggtt atccaccata acttcattga tcacccaacg
     2461 cggattttca tcttttatgg tacaactcac aagaagtatc aatgcagaca aaaacaacag
     2521 ttgttttaaa ttattcatac ttatgtaaat tatagcggta aattatcgtg ttttttggga
     2581 ggattgacca gctttttggt ctgtaattgc tgcaatgctc tgataacacg gaaacgggta
     2641 tttctcggct caatcacatc gtcgatatag ccgtaacggg ctgcttcata cgggttggtg
     2701 aacaagtcgt tgtattcttt tttctttgtt tccagaatga gagccagttt ttcgggatct
     2761 ttttcggcag caatttcctt gctgtaaaga acctctacag ctccttcggc acccatcaca
     2821 gcgatttcag cacttggcca agcatagtta atatcaccgc gaagttgctt gcagctcatg
     2881 acgatatgtg ctcctccgta cgacttacgc agtgtgacgg tcactttcgg aacagtggct
     2941 tcaccgtatg cgtatagcag ctttgcaccg tgagtgatta cgccgccata ttcctgaccg
     3001 gtaccaggaa ggaaaccggg aacgtcaacc agcgaaacaa tgggaatgtt gaaagcatca
     3061 cagaaacgga caaacctggc tgccttgcgt gaagcgttaa tgtcgagaac ccctgccaaa
     3121 aacttgggtt ggtttgctac aatacctacc gattgaccgt tgaaacgagc gaaacccacg
     3181 atgatgtttt ttgcatagtc agcatgcact tccagaaatt ctccactgtc gacaatggct
     3241 ccaataattt cgtacatgtc gtagggtttg ttggcactgt ctgggataat ttcgttcagg
     3301 ccgtcctcca acctgtcgat cgggtcggag cttttaaata ccggcggttc ttccaggtta
     3361 ttctgaggaa tgtacgacaa cagtttctga atcatctgta tagcatcctc ttcggtctga
     3421 gcggagaaat gggccacgcc cgatttggaa gtatgcaccg aagcgccacc cagttgttcc
     3481 tgtgtaacat cttctccggt aacggttttt accactttgg gaccggtaag gaacatgtag
     3541 gaagttccct gggtcatgat gttgaagtca gtaagagcag gggaatatac ggcacctcct
     3601 gcacagggac cgaagatacc tgaaatctgt ggaatcactc ccgacgctaa aatgttacgt
     3661 tggaaaatct cggcataacc tgccagtgca ttaattccct cctgaatgcg tgcacctccg
     3721 gagtcgttga taccgataag aggggcaccc attttcatag cctgatccat tactttgcaa
     3781 attttttgcg attgcatttc ggatagcgaa ccgccagaaa cggtaaagtc ctgggcaaaa
     3841 acataaacca ggcgtccttg gatagtgccg tatccggtaa caactccgtc gccaaggaat
     3901 ttggtttttt ccatgccgaa gttgtgacaa cggtgttcca caaacatgtc gaattcttca
     3961 aaacttcctt cgtcaagaag catggccaga cgttcgcgag ctgtgtattt accttttagg
     4021 tgctgagact cgattctttt ctcaccacca cctaagcgag ctttttctcg taattgaata
     4081 agctctttaa ctttttcgag ttggatgctc ataagattat aatttgttat gaattaaatt
     4141 tttgttttat tgttacctcg atagcctcct caaatcacta tcgaggttgg tcatctacgg
     4201 atgctcgcac aattcggtca atacgctacc ggttgatttg ggatgtaaaa aagcaatgtt
     4261 cagtccttcg gcgccggcac ggggttgcgt atcgataagg cgtacgcctt ttgcttcaat
     4321 ttctcggaga gactcaccca ggtttttagt ggcaaaagcg atgtgatgaa taccttcacc
     4381 tcttttttca atgaatttgg caatggtact ttcttcgctg gtaggttcca atagttctat
     4441 ttttgtttgg ccgatcataa aaaaggccgt ttttactttt tggtcttcaa ccgtttcgat
     4501 gttgtagcat tttagaccaa gaattccttc ataataagga agttgttctt caatgttttt
     4561 tacagcaatg ccgatgtgtt caatgtgtgt taattccata ttacttaatt aagtgattat
     4621 ttttttaaaa atgcaaaggt aagactttta agttgtatga taaaatttat ttttttcgtt
     4681 ttaaatggaa gaaatttcaa gtacttcgag cagttcagga ctgatttccc gatctttttt
     4741 tatggcacgt ttaaacggta catagtcgat ttcgttttca cgaataccga tcattacatt
     4801 gcgttgattt tccagcaacg cctggatggc tgctatgccc atcctgcttg ccaggatacg
     4861 gtcctgtgcc gtgggtgaac ctccacgttg caaatgtcct aaaatggaga cgcgcacatc
     4921 gtactgagga tactgttttt ttaccctctc agccaggttc atcgcgccac ctgtaatttc
     4981 actttcagta acaagaacca tgctgctgct tttcgatttt cggaaaccct gatcgataag
     5041 ttccgccaac tggtctttct ccatgcttat ttcagggatt atggcggctt ccgcccccga
     5101 ggctatagcg ctgttgagtg ccagatagcc acaattacgt cccatcactt ccacaaagaa
     5161 gagtcgttcg tgggatgtgg ctgtatcgcg aattttatcc atcgattgca taatggtatt
     5221 tagggcagta tcgtaaccaa tggtggtgtc ggttccgttc aggtcgttat caatggttcc
     5281 tggcaaccca acaatcggaa tattgtattc attggcaaat actccggcac cagtcagtga
     5341 tccgtcgccg ccgatgacca caagcgcatc tattccgtgt cgttgcatgt tttcgtaagc
     5401 tgtctttctt ccggcttcag tcataaactc ctcacatcgg gcagtcttaa gtattgttcc
     5461 gccctgttgg attatattgc ttacgctgtt ggtcttaaac tcttctattt cgttgctaat
     5521 aagtccctta tagcctctgt agatgccaaa aacgcgcata ccgttgaaaa ttgcagcgcg
     5581 cgttacagcg ccgatggcag cattcatgcc cggtgcatct cctccggaag taagtacgcc
     5641 gatgcttttt acatttgaat ccatttcgaa aattagttct ttttttaaaa ttggttgaac
     5701 ctgaatacaa tcggctgcaa aggtaatttt tttttaggag gcgcacaaaa aaaagctgct
     5761 ttaaaaagca gctttttcaa ctttcatttc cggtataagc tccgatattt tttttgcgac
     5821 aacctccatc tgccacatgg gtgttgaggt agctccacaa atgccgatgc tgatgtcatc
     5881 ccgtatccat tccgtatcca gttggtcagg atgatggatc aagtgtgagt tccggttgta
     5941 cttttcacat tcggcaaaaa ggacttttcc gttggaactt ttttctcctg cgataaaaat
     6001 aacgaggtcg tgattcttag caaattcttg catgttgggt acgcgattgg aaacctgacg
     6061 acaaatggta tcataaaatt caaatgtggc accatttttc atccgttctt ttatcaattc
     6121 ccctatttcc tgaaatccgt ccagcggttt cgtcgtttgt gaaaagaggc ttatgtcgcg
     6181 tgagaagtcg agtttgtcta tgtcttcttt cttttcgatt actatggctg attcatcggt
     6241 ctgtcccatc aatccattta cctcggcatg gccttttttt ccgaatatca ctatttgtcc
     6301 atcttcattt tgtttggtga taaatttctt tttgattcgt ttttgcaacc caagaacgac
     6361 cggacaggat gcgtcaatca agtctatatt gttttctttt gctatttggt aagtgcttgg
     6421 gggctccccg tgggcgcgaa gcagaacgcg gacatccctg agttttttaa attcatcgtg
     6481 gttgatggta atcaatccca ttttggcgag acgctccact tcaatgttat tgtgaacgat
     6541 atcgcctaaa cagtaaagag ttccgccttt tttcagttct tcctcggctt tgctgatagc
     6601 tttggctacc ccaaaacagc agccggactg cttgtctatc tcaattttac tcattttact
     6661 ttgtgatttt gttaaacata tccattgccc attgcaattg ttctgtaata gttatatgtg
     6721 aattatccag ttcgatagca tcggtcgctt ttcgtaacgg actctctatg cgggtggtgt
     6781 ctctttcatc tctttcgagg acgttggcga gggtgtcttc ataggtaaca ggattgcctt
     6841 tggcctgaag ttcttcgtaa cggcgttgtg cgcgaacttc gggtgatgcc gtgagaaaaa
     6901 ttttgaattc cgcgtcaggg aaaactactg tgccgatatc cctgccgtcc atcactaccc
     6961 ctttcttttt ccccattgcc tgttgctggc gtaccaactc tctgcgtaca aatcccaaag
     7021 cactcacgcg gcttgcaccg ttccctattt caagcgaacg gatttctttt tctacatttt
     7081 caccgtttag ataagtttcc tgttggcctt ctgaatttgt tttaaaggaa atatctattt
     7141 ctgagatatg catttgtaat gcagcatcat caatttccgt atcggttatc catcccttac
     7201 ggtatgcaaa cagagctact gccctgtaca tggctccgct gtcgatataa gtataaccta
     7261 attcccttgc caatcccttg gctatagtac ttttgccgca tgatgaaaag ccatctacgg
     7321 cgatatttat ttttcccatt cttattgaaa gcagatactg tttttataaa caactacaaa
     7381 gatagaatgt tttcgttata attggtttta acacaaaatc tagccaaaca attcgattgc
     7441 ttttttctcg tatttgggaa tcttgaataa ctgctgtccg gttgcaaaga aaagcgattt
     7501 taacctcccc gatttaatca tcagattaat taactgctgg tgtcgtttgt gcttatgaat
     7561 aacagccggc attttatcgg agatatacga tgggttgaga atagtttttg cagtttcttc
     7621 tcccgatata agtgcttggt aaatcccttc gccgatgaac ggagaaacca gtccggcggc
     7681 atcaccggtc agaaatatgt tgtcaaaacg gattccgtta taatcggcat ccatggggaa
     7741 agcttcgtat tttcctttgg aaatatcgat ctgtctgtcg ttgagccatt gatgaaaatt
     7801 ctcctttaat tttttcggcg atacatgttt cggatcggcg ccgcaaccga cggagatata
     7861 gctgcgatgc ggaaaaatcc acgcgtacca tgccgaaaag tattcaggct gaaagaatat
     7921 ttcgaaatct tggtacttgt tggtggaaat tatgtattga ataccaataa ttttccttct
     7981 tgactttaaa ccaagaaagg ttttcacgat agaattggat ccatctgctc caacgagata
     8041 tttaaagcct attttttccc caccgacaac aatgaagtct ttgttgacca ccgtagcctt
     8101 gctgctgatt cttatttcga tattggggaa tgcctttatt ttttgtaatt gccactgtcc
     8161 gaattttttc ctatcgatag taaaagcaaa at
//
LOCUS       DAOSDR010000089         8177 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrimonas sp. isolate AMR_MDS_1826
            NODE_3265_length_8177_cov_3.692440, whole genome shotgun sequence.
ACCESSION   DAOSDR010000089 DAOSDR010000000
VERSION     DAOSDR010000089.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863355
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrimonas sp. (wastewater metagenome)
  ORGANISM  Petrimonas sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Dysgonomonadaceae; Petrimonas.
REFERENCE   1  (bases 1 to 8177)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8177)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:10:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,720
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 35
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8177
                     /organism="Petrimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3265_length_8177_cov_3.692440"
                     /isolate="AMR_MDS_1826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2023866"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..467
                     /locus_tag="PLI30_04995"
     CDS             <1..467
                     /locus_tag="PLI30_04995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006844490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phosphoribosylglycinamide synthetase C
                     domain-containing protein"
                     /protein_id="HOI78946.1"
                     /translation="KGFIFFGLINIDGEPKVIEYNVRMGDPETEVVLPLVKSDILDLF
                     VSVATETLDTKTVEIDNRYAVTVMMVSGGYPGSYEKGKTISGIEQIKDSIVFHAGTTL
                     KNDEPVTSGGRVLAVTSYGHTMNEALENSYKNIEKISFENSYFRKDIGFDLR"
     gene            509..1495
                     /locus_tag="PLI30_05000"
     CDS             509..1495
                     /locus_tag="PLI30_05000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI78947.1"
                     /translation="MESRFISLVVSITVIALRFLMFLKKGLPDFPANNTGFIWPYIEP
                     VFLENPLLSFLASTFCVFIISYLLSELNVRYGVIRMRTAMPFYVPLILFSVHPFFLRM
                     TPDFPGLIFVLWSLFPLLASYQYHHSHRFAYQFSALIAIAGIFQIHALLFVPLWLIGL
                     SAMGRINFRSFIASIFGIILVFWIAFVFYVFGDNISGFIEPFKGLAEIYNFTRTPGFS
                     VPQWGFIGTMLLFLFFIITADSKQITRERSFTKKVLIFSISVILLSFLLQILYLTHTF
                     IWIHIIIAFLSIIVAHFYANTTFRLEILSYFFVLMLLAFYYCLNLFTDLSPF"
     gene            1712..2200
                     /locus_tag="PLI30_05005"
     CDS             1712..2200
                     /locus_tag="PLI30_05005"
                     /inference="COORDINATES: protein motif:HMM:NF025425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4251 domain-containing protein"
                     /protein_id="HOI78948.1"
                     /translation="MKRSALHLLIVLSVAFGLTMCGTAQTAREKEVVAGEVSRSVNDF
                     DFTFKATYAYPTGFKSIYLSPYYDVKVSPDTIRAYLPYYGRAYVAPVNPSEGGIKFTS
                     TDFDYQVNPGKKKGNWRVDIRTKDTGREIFLYFDIWENGTARLQVTDTNRQPISFQGD
                     LL"
     gene            complement(2195..3826)
                     /locus_tag="PLI30_05010"
     CDS             complement(2195..3826)
                     /locus_tag="PLI30_05010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006798597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase"
                     /protein_id="HOI78949.1"
                     /translation="MRKLSLLFTLLISTVLMLAQEVRPVKNVIVMIPDGTSVGVYSAS
                     RWYKFYNKMGDRLHIDPFFTGTVTTHSSNAPIGDSAPTGSAYATGVLQKTSNVAIYPE
                     ADPENDLYPVDAARTYQPAATLLEAAKLMKNKAVGLVVTCEFPHATPADFSSHYHTRS
                     AYKFIAPQMAYQNMDVMFGGGNSILTDDIRQHFKNNGTVLIQDDRNALLNYNGDGKVW
                     ALFGERALPYSIDRNPDNVPSLAEMTAKALDLLSKKEAGFFLMVEGSQVDWAAHANDA
                     VGMITEYLDFDDAVGEVMKFAEKDGNTAVIIMSDHGNSGFTIGSRDCPGYDKLSIQQL
                     FEAVSNYKLSANGIEAILINTKPENIKAEFKKYTGIDITEEELQTLLSSKNYKESDYT
                     QVGTSNNMAHNIVNIMNSRTCFGFTTGGHTGEETLLASYHPQGDILRGNVRNVQVNKY
                     LQKALGLDKSLQELSDEIFVKHTDVFAGQKYSVNRKDPEFPVLTVKKGRNTLEIKAFS
                     SVGKLNGKPFDIGSVAVYMDKNDTFYLPKELVTKL"
     gene            3989..4756
                     /gene="surE"
                     /locus_tag="PLI30_05015"
     CDS             3989..4756
                     /gene="surE"
                     /locus_tag="PLI30_05015"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019539379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008252 - nucleotidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HOI78950.1"
                     /translation="MNSRKPLILVTNDDGYQAKGIVSLIEAMRALGEVVVFAPELHQS
                     GMSSAISTSQPLRSRIYKKEEGLTAYMCTGTPVDCVKLALNEFLDRKPDLLVSGINHG
                     SNAGISVIYSGTMGAAIEGCIFEVPSLGVSLCEYAYDADFTHACEVAQRIASTVLKKG
                     LPKGICLNINVPQGEIKGIKITSQTQGKWVNEYQRSADGNGRDVFWMTGNFENWEEDN
                     VNTDEWALANGYAAIVPVKIDMTAHDFIPELKKWDLT"
     gene            4761..5888
                     /gene="lpxB"
                     /locus_tag="PLI30_05020"
     CDS             4761..5888
                     /gene="lpxB"
                     /locus_tag="PLI30_05020"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008143835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008915 - lipid-A-disaccharide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HOI78951.1"
                     /translation="MKYFLIAGEASGDLHASNLMKSIKQLDKEADFRFFGGDLMAVQG
                     GTLVKHYREMAFMGIVPVVMNAKTILNNLQLCQKEIAGYQPDAVILVDYPGFNLKMAR
                     FVKARLNIPVFYYISPKVWAWKEYRVKSFKKYVDEMLCILPFEVDFYEKHDYRVHYVG
                     NPSVDEIDAREYKDETFAGFIAANTLPDRPIIAVLAGSRKQEISSNLPPMLQAVKGFD
                     DYQLVVAGAPGIEPDFYDKFTQGFPLRVLFHQTYRILAQSQAALVTSGTATLETALLN
                     IPQVVCYRTSMPRLSYWAFKNILHTPYISLVNLICGREVVTELFAKFFTVDNIRKELT
                     QLLHVKNYRKNMLDNYAEMRRILGGTGAADRAAETILNKLR"
     gene            5996..7018
                     /locus_tag="PLI30_05025"
     CDS             5996..7018
                     /locus_tag="PLI30_05025"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019539376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003872 - 6-phosphofructokinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006002 - fructose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent 6-phosphofructokinase"
                     /protein_id="HOI78952.1"
                     /translation="MKIGILTSGGDCPGINATIRGVGKTAINNYGMKVIGIQNGFSGL
                     LYKDVIDLSDASLSGILNLGGTILGTAREKVFRKKITSPDEKDQEEIKKCYHELGLDC
                     LVCIGGNGTQKTTWMLSELGLNVVGIPKTIDNDVYGTDVTFGFDTAVNIATDAIDRLH
                     STASSHRRVMVIELMGHHAGWITLYAGMAGGADIILLPELGYNMKVIIDKIKKRMEMG
                     KAYSIVAVAEGIEIQGSSERPAMYFAREIERQTGFETRETVLGYIQRGDSPSPADRNL
                     GTLLGGHAAQLIHEGKFGRMVAQIKNKIDDVPLEEVAGKLRLVTVDTPLVQQGRRMGI
                     SFGVWV"
     gene            complement(7027..7833)
                     /gene="rnc"
                     /locus_tag="PLI30_05030"
     CDS             complement(7027..7833)
                     /gene="rnc"
                     /locus_tag="PLI30_05030"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006843811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HOI78953.1"
                     /translation="MRKVRAIPHKGKEPYFSFYRMLGFYPDRIDLYQEALTHRSSSIR
                     SNSGKWVNNERLEFLGDAILDAIVADILYKKFENKKEGFLTSTRSRIVQRETLNKIAI
                     EIGIDKIITSSTRNLAHNTNIYGDALEALIGAIYLDQGYRVAKSFVFETLIKQHINID
                     TVLKSEVDFKSRLIEWGQKYKIDVGFEVTDSSYDEQNNPIFESRVIVGGMDLGSGKGY
                     SKKESHQKAAKKAIKKLRNDNELLEKLFEKGNSESSETSGQEEENVQIEI"
     gene            complement(7850..>8177)
                     /locus_tag="PLI30_05035"
     CDS             complement(7850..>8177)
                     /locus_tag="PLI30_05035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005645359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="beta-ketoacyl-[acyl-carrier-protein] synthase
                     II"
                     /protein_id="HOI78954.1"
                     /translation="GDISEVKAIKEVFGEHSYKLNISATKSMTGHLLGAAGSLEAMFC
                     VLALENQIVPPTINFTEGDEDPEIDYNLNFTFNKAQKRELRAVLSNTFGFGGHNASVI
                     LKKFNK"
BASE COUNT         2239 a         1808 c         1754 g         2376 t
ORIGIN      
        1 acaagggttt tatcttcttc gggttgatta atatagatgg tgagccgaaa gtgattgaat
       61 acaatgtgcg gatgggtgat cctgaaacgg aggtggtatt gccgcttgtc aagtcagaca
      121 ttctggacct ttttgtaagt gtagccaccg aaactctgga tacaaaaaca gtggaaatcg
      181 ataacaggta cgctgttacc gtgatgatgg tttctggggg atatccaggc agttacgaaa
      241 aggggaaaac aatatcggga attgaacaga taaaggattc cattgttttt catgccggga
      301 caacgttaaa aaatgacgaa cccgtaacat caggcggacg tgtcttagct gtaacctcat
      361 atgggcatac aatgaatgaa gcgcttgaaa attcctataa aaatatcgaa aaaatctctt
      421 tcgaaaacag ctatttcaga aaagacatcg gctttgactt aagataaaaa tgaacacttt
      481 gctggttaga aacttcaaat ctttttttgt ggaatccaga ttcatttcac tagttgtttc
      541 tattaccgtc atcgcgttgc gttttctgat gtttttgaaa aagggcttac ctgattttcc
      601 ggcaaacaat accggcttta tctggcccta tatcgaacct gtttttctcg aaaatcccct
      661 tctctcgttt ttagcgagca cattctgtgt tttcattata tcttatcttc tttccgaatt
      721 gaacgtccgc tacggtgtga tccgcatgcg aacagcgatg cccttttatg ttccgctgat
      781 tctatttagt gtccatccgt tttttctgag aatgactccc gattttccgg ggctcatctt
      841 cgttctctgg tccctttttc cgttactggc atcgtatcag taccaccatt cccaccggtt
      901 tgcttatcag tttagtgcgt tgatagccat tgccggaatc tttcaaatcc acgcgttatt
      961 gtttgtgccc ttgtggttaa ttgggttatc ggcgatgggc agaataaact tccgatcttt
     1021 tatagcatct atttttggaa taatactggt gttttggatt gcttttgtgt tctatgtctt
     1081 tggggataac atctccggct ttatcgaacc ttttaaaggt ctcgccgaga tctacaactt
     1141 tacgcggacg cccggttttt ctgtcccgca atggggtttt atcgggacaa tgctgctttt
     1201 cttgtttttc attattaccg ctgatagcaa gcagattaca agagagcgga gctttaccaa
     1261 aaaagtatta attttcagca tttccgtaat actgctttcc tttcttttac agattttgta
     1321 cctgactcac acgttcatct ggatccacat tatcatcgca tttctttcca tcattgtggc
     1381 gcatttttac gccaacacca cattcaggct ggagatttta tcatatttct tcgttttgat
     1441 gctgttagct ttttattact gcctaaacct tttcaccgat ttatcaccat tctaagaaac
     1501 gtcttaaatc ttaaatttct attcatctct acttccttca tgctgttttt cgctcttttt
     1561 cgcgtccttg aatcttcatt tcttcaaaat agccccacta tagatccatc aaaaaataaa
     1621 cataaaacaa gacatgaaaa aaatcaataa gttgctaaca acaaaaccct atgtattttt
     1681 gttttacagt aaaaaacaaa aaaatataca gatgaaacga tcagcattac atttacttat
     1741 tgtgctgtct gtcgcttttg ggctgacaat gtgtggcaca gcacagacgg caagggagaa
     1801 ggaagttgta gccggagaag tgagccggag cgtaaacgat ttcgacttta cgtttaaggc
     1861 aacttacgct tatcccaccg gtttcaaatc catttacctg tctccctact acgacgtaaa
     1921 agtatctccc gacacgatac gggcatacct gccctattac ggacgagcat atgtagctcc
     1981 cgttaatcca agtgagggag gaataaaatt taccagtacc gacttcgatt atcaggtgaa
     2041 tccgggaaag aaaaaaggga attggcgggt agatattcga acgaaagata ccggccggga
     2101 gatatttttg tatttcgata tctgggagaa cggaacagcc cggttgcaag tcacagatac
     2161 caaccggcaa cccatctcgt ttcaggggga tttgttataa cttcgttact aactccttag
     2221 gcaaataaaa ggtatcgttt ttatccatat acaccgcgac cgatccaata tcgaatggct
     2281 tgccgttaag ttttccaacg gacgaaaaag cctttatctc cagcgtgttt cttccttttt
     2341 taaccgttag gaccggaaac tcgggatctt tccggttcac ggagtatttc tgcccggcaa
     2401 aaacatcggt atgcttaaca aagatttcat ccgacagttc ctgtaatgat ttatccaacc
     2461 ccaatgcttt ttgcagatac ttgttcactt gcacattgcg cacattccct ctcaggatat
     2521 ccccttgcgg atgatacgat gccaacagtg tctcttcgcc ggtatgccct cctgtggtaa
     2581 atccgaagca ggtacgcgaa ttcattatgt ttactatgtt gtgtgccatg ttattacttg
     2641 tccccacctg ggtgtaatca ctttctttgt agtttttcga agagagcagt gtctgcagtt
     2701 cttcctcggt aatatcaatg ccggtgtatt ttttgaactc ggcctttatg ttttcgggtt
     2761 tggtatttat cagaatggct tcgatgccgt ttgccgaaag tttgtaattc gataccgctt
     2821 caaacaactg ctgaatcgac agtttgtcgt accctggaca atctcttgag ccaatggtaa
     2881 aaccactgtt accgtggtca gacataataa tgaccgccgt atttccgtcc ttttcagcaa
     2941 acttcatcac ctcccccacg gcatcatcaa aatccaggta ttcggtaatc atcccgacag
     3001 catcattagc atgagcggcc cagtccacct gactgccttc aaccatcaga aaaaagcccg
     3061 cttctttttt ggataaaaga tccagcgcct ttgcggtcat ttctgcaagc gacggaacgt
     3121 tgtcggggtt cctgtcaatg gaatagggca atgcccgctc tccaaaaagc gcccagactt
     3181 ttccgtcacc gttgtagttc aacaacgcgt ttctatcatc ttgaatcagc acggttccgt
     3241 tatttttaaa gtgttgcctg atatcatcgg ttaaaatact gtttcctccg ccgaacatca
     3301 catccatatt ctggtaagcc atttgcggtg caataaactt ataggcactg cgtgtatggt
     3361 aatgcgaaga aaaatcagca ggcgtagcat gagggaactc acaggtcacc accaacccta
     3421 cagctttatt tttcatcaac ttagctgctt ccaacagggt agctgcaggc tgataggtgc
     3481 gggcagcatc caccggataa agatcgtttt ccggatctgc ttccgggtaa atggccacgt
     3541 tgcttgtttt ctgtaaaaca cctgtcgcat aggctgaacc cgtaggagcc gaatcaccaa
     3601 taggcgcatt ggaggaatgt gttgtaaccg ttcctgtgaa aaaggggtcg atatgcaggc
     3661 ggtcacccat tttgttgtaa aatttatacc agcgggaagc agaatatacg cccaccgagg
     3721 taccgtcggg aatcatcaca ataacgtttt ttaccggacg cacttcctgt gctaacatca
     3781 aaactgtact tatcagcaaa gtaaacagca agcttaattt tctcatatgt tgaattgtat
     3841 ttatacttga gtttaaaaca ataaagttaa taaatagttg aatttttttg aaacataaac
     3901 ttcaatttaa cgattttgta acacctccta tgtatgaaaa gtcgatgaat ttgattaagt
     3961 ttgtccttca tttctaaaga aaaaaataat gaacagtaga aaacctctta tccttgtcac
     4021 caatgacgat gggtatcagg ctaaaggaat agtatcgctt attgaagcga tgagagcatt
     4081 gggcgaggta gttgttttcg ccccggaatt gcatcagtcg ggaatgtcgt ctgccatctc
     4141 cacatcacaa ccgttgagaa gcagaattta taaaaaggaa gaaggactta cggcatatat
     4201 gtgcacagga actccggtgg attgtgtaaa attggcgctg aacgagtttt tagatcgcaa
     4261 gcccgactta ttggtttcgg gcatcaacca cgggtccaat gctggaatta gtgtaatata
     4321 ctcgggaacg atgggcgctg ctatagaagg atgtattttt gaagtcccct cccttggcgt
     4381 atctctttgt gaatatgcat acgatgccga cttcacgcac gcctgtgaag tagcccaaag
     4441 gatagcctcg actgttctca aaaaggggct tcccaaaggt atctgtttga acataaacgt
     4501 tccacaaggg gaaataaagg gaataaaaat aacatcacag acgcagggga agtgggtgaa
     4561 tgaatatcaa cggtctgctg atggaaacgg ccgcgatgtg ttttggatga ccgggaactt
     4621 cgaaaactgg gaagaagaca acgtaaatac cgatgaatgg gcattggcaa acggatacgc
     4681 tgccattgtc cctgtaaaaa ttgacatgac agcacacgat tttatccctg agctgaaaaa
     4741 gtgggatttg acataagaat atgaaatact tcttaatagc cggcgaagca tcgggcgacc
     4801 tacacgcatc caacctgatg aagtcgatca aacagttgga caaggaagcc gatttccggt
     4861 ttttcggagg tgatcttatg gccgtccagg gaggaacact ggttaagcat taccgtgaaa
     4921 tggcatttat gggaattgtc ccggttgtta tgaatgcgaa aaccattctt aacaacctgc
     4981 aactctgtca aaaggagatc gccggctacc agccggacgc ggttatcctg gttgattatc
     5041 cgggattcaa cctaaagatg gctaggtttg taaaggctcg attgaacatt cccgtcttct
     5101 attacatctc acctaaagta tgggcatgga aagagtatcg ggttaaatcg ttcaagaaat
     5161 atgtggatga gatgctttgt atcctgccct ttgaagtaga tttctacgaa aaacacgatt
     5221 accgggtcca ttacgtcgga aatccgtcgg tggatgagat cgacgcgcgc gaatataaag
     5281 atgaaacgtt tgccgggttc attgcggcca acacacttcc cgaccgtccg attatcgcag
     5341 tgcttgcagg aagcaggaaa caggaaatat ccagcaacct gccacctatg ctgcaagcag
     5401 taaaaggatt cgacgattac caactggtag ttgcaggggc acccggtatt gaacctgatt
     5461 tttacgataa gttcacccaa ggatttccgc tgagggtttt gttccaccaa acgtaccgca
     5521 ttctcgccca gtcacaggca gcgttggtta cctccgggac ggccaccctg gagacggcgt
     5581 tgctgaacat tcctcaagta gtttgttacc gtacgtcgat gccgcggcta tcatactggg
     5641 ccttcaaaaa catcctgcac acaccctata tctcattggt gaaccttatt tgtggcagag
     5701 aagttgtgac ggaactcttc gccaagtttt tcaccgttga caacatccga aaagaactta
     5761 cccaacttct ccacgtgaag aactaccgaa aaaacatgct ggacaattac gcggaaatgc
     5821 ggcggatact gggcggaacc ggtgctgcgg accgtgcagc ggaaacaata ttgaataagc
     5881 tcaggtgaat cgattttctg ttggcatact gaaaatattc tttccaaaat cttcactttt
     5941 taatcctttt ttgtttcctt tgtatcggaa ctaatgtaag aaaatcagaa acagtatgaa
     6001 aattggaatt ctcacttccg gtggtgactg tcccggaatt aatgcgacca tacgaggcgt
     6061 tggaaaaaca gccattaaca attacggaat gaaggtaatc ggcattcaga atggattttc
     6121 cggactgctt tacaaggacg ttattgacct ttccgatgca tcgctctctg ggattcttaa
     6181 tctgggagga accattcttg ggaccgcacg cgaaaaggtg ttccgtaaaa aaatcacatc
     6241 acccgacgaa aaagaccagg aagagataaa aaaatgctac cacgaattgg gcctcgattg
     6301 cctggtttgt attggaggaa acggcacaca aaaaaccact tggatgcttt ctgaattggg
     6361 gctgaatgta gtaggaattc ctaaaaccat cgataatgat gtgtacggaa ccgatgtcac
     6421 tttcgggttc gacaccgctg tcaatattgc tacggatgct atcgaccgcc tgcattccac
     6481 ggcaagttcc catcgtcgtg taatggttat tgaacttatg gggcaccatg ccggctggat
     6541 tacactgtat gctggaatgg ccgggggagc cgatatcatt ctacttcccg aacttggtta
     6601 caacatgaaa gtaattatcg acaagataaa aaagagaatg gaaatgggga aagcctattc
     6661 cattgtggct gttgccgagg gaattgaaat acaaggaagc agcgaaaggc ctgccatgta
     6721 tttcgccaga gaaattgaaa ggcaaaccgg atttgaaact cgcgaaaccg tcctcggata
     6781 cattcagcgc ggagattcac cgtctcccgc agaccgtaat ttaggcactt tgctcggcgg
     6841 ccatgcagca caactcattc acgaagggaa gttcggacgt atggttgcac agatcaaaaa
     6901 taaaatagat gacgtacctt tagaagaagt tgcgggcaaa cttcgcctgg taactgtgga
     6961 tactcctttg gtacaacagg gaagaaggat gggtatttca ttcggcgtct gggtataagc
     7021 tggacattag atttctatct gcacgttctc ttcctcctgc ccggaagttt ctgaagactc
     7081 actattccct ttctcgaaaa gtttttccag aagttcatta tcgttccgca gcttcttgat
     7141 ggctttcttt gctgccttct gatgcgattc ttttttggaa tacccttttc ctgaacccag
     7201 atccatccca ccgacaataa ccctcgattc aaaaatagga ttgttttgct catcgtagga
     7261 agagtcggtc acctcaaacc ctacgtcaat tttgtatttt tgtccccact ctatcagacg
     7321 cgacttaaag tctacctcac ttttcaatac ggtatcgata ttgatgtgtt gcttaatgag
     7381 cgtttcaaaa acaaaacttt tcgctacgcg gtaaccctga tcaagataga tcgctccaat
     7441 cagtgcttcg agggcatctc cgtagatatt ggtgttgtga gcaagattcc gggtggatga
     7501 agtgatgatt ttatcgatcc ctatttcaat agcaattttg ttcagcgttt cacgttgaac
     7561 aatcctcgag cgggtactgg taaggaatcc ctctttttta ttctcgaatt ttttgtacaa
     7621 aatatcggct acaatggcat caagaatagc atcacccaga aattcgagcc gttcattatt
     7681 aacccatttt cctgaatttg agcgaattga tgatgatctg tgtgtcaatg cttcctggta
     7741 cagatcaatt ctatcgggat aaaatcccaa catcctgtaa aatgaaaagt agggttcttt
     7801 tcctttgtga ggaattgccc ttacttttcg cataaatttt cttaacacat tacttgttga
     7861 atttcttaag gattacgctt gcgttatggc ctccaaagcc aaaagtattc gatagaacag
     7921 ctcttaactc gcgtttttga gctttgttga atgtgaagtt aagattataa tcgatttccg
     7981 gatcctcgtc gccttcggtg aaatttattg ttggcggaac aatctggttt tcaagagcaa
     8041 gaacacaaaa catagcttcc aatgaaccgg cggcacccaa caagtgtccc gtcatcgact
     8101 tagttgcact tatgttcagc ttgtaactgt gttccccaaa aacttccttg attgctttca
     8161 cttccgaaat atcacct
//
LOCUS       DAOSDR010000090         8136 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrimonas sp. isolate AMR_MDS_1826
            NODE_3280_length_8136_cov_4.130182, whole genome shotgun sequence.
ACCESSION   DAOSDR010000090 DAOSDR010000000
VERSION     DAOSDR010000090.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863355
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrimonas sp. (wastewater metagenome)
  ORGANISM  Petrimonas sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Dysgonomonadaceae; Petrimonas.
REFERENCE   1  (bases 1 to 8136)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8136)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:10:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,720
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 35
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8136
                     /organism="Petrimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3280_length_8136_cov_4.130182"
                     /isolate="AMR_MDS_1826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2023866"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..199
                     /locus_tag="PLI30_05040"
     CDS             <1..199
                     /locus_tag="PLI30_05040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019540925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="L-sorbose 1-phosphate reductase"
                     /protein_id="HOI78955.1"
                     /translation="NNLPNIPGGKKLIYTHLTMPLTPITDFEKLGKTSELFKELANIC
                     NNHKGMWSVEAENYLLANIVK"
     gene            598..3042
                     /gene="thrA"
                     /locus_tag="PLI30_05045"
     CDS             598..3042
                     /gene="thrA"
                     /locus_tag="PLI30_05045"
                     /EC_number="1.1.1.3"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005861780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009067 - aspartate family amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase I"
                     /protein_id="HOI78956.1"
                     /translation="MKVMKFGGTSAGTPESMLLVKNIIEAEKEPVIVVVSALGGVTDR
                     LLLAADFALNANPGYQSILEEIIFRHEEMIGKMAVSAVDQKELRQKIDPMFEDLRNIL
                     RGVYLIGDLSQKTSDKIVSYGERFSSLIVSKIIDGARVYDSTQFIKTTKQFSHHIPDI
                     AYSNELIKQAFLSDPLPKVAIVPGFISSSKEADDITNLGRGGSDYTAAIIAAALNASV
                     LEIWTDVDGFMTADPKIINSAYVIEELSFTEAIELSNFGAKVIYPPTIFPVYHKSIPI
                     RVKNTFKPASEGTLIRNIKPTPNGKIIKGISSINDTALITIQGLGMVGVIGVNKRIFT
                     ALADNGISVFLVSQASSENSTSIGVRTQDAPLSQKVLSKEFAKEIERGSINEIIVEYD
                     LATIAVVGQNMKHVPGVAGKFFGTLGRGGISVVALAQGASETNISCVIAKTNLKKALN
                     IIHDSFFLSPYQELNLFVIGTGTVGSKLLAQIKQQKHILEEQNKLKINIVGIANGRKA
                     LFSRDGISLENYFENLMSRGVKSSPQLIRDEILKMNIFNSVFVDCTASQAISELYESL
                     ISRNISVVTANKIAASSDYKNYHHLKETARKTGTKFLFETNVGAGLPIINTMNSLTNS
                     GDKIVKLQAVLSGTLNFIFNTLSEDVPFSKAIKMAVDAQFAEPDPRIDLSGLDVTRKL
                     VILSREAGAQVEQENVQKHLFIPQKYFESTLDEFWSTIPEMDEAFENRRKKLDKEGKK
                     LRFIATYDNGLCEVGLHEVEKGHPFYDLEGSNNIIMITTERYNEYPMVIKGYGAGASV
                     TAAGVFSDIISIANIR"
     gene            3053..4258
                     /locus_tag="PLI30_05050"
     CDS             3053..4258
                     /locus_tag="PLI30_05050"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013617383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cofactor-independent phosphoglycerate mutase"
                     /protein_id="HOI78957.1"
                     /translation="MKYIIILGDGMADEPVAKLGNRTPLQAAKTPYMDRLAQKGRSGM
                     LDTIPEGMSPGSEIANLSVLGYDVPRVYEGRGVLEAASMGVDILPDEMAMRCNLVTIR
                     GDVLKNHSAGHISTAEAAELIHFLDDRLGNDRFRFYPGVSYRHLLKMKGGNKNIVCTP
                     PHDVPDKPFYPLLIKPAEEPAQSTADELNRLILASQEILPAHPVNQKRIALGKDPANS
                     IWLWSPGYRPGMQPLNKKYPIQKGAVISAVDLIRGIGVYAGLEVIQVEGATGLYDTNY
                     EGKARAALDALKENDFVFLHIEASDEAGHEGDVDLKVRTIEYLDSRIVKPIFEETSTW
                     DEPVTIAVLPDHPTPCAIRTHTRDAVPFVVYHKGIEPDSVKTYDEFAAKKGVFGLLRG
                     DEFMKNLIL"
     gene            4262..5566
                     /gene="thrC"
                     /locus_tag="PLI30_05055"
     CDS             4262..5566
                     /gene="thrC"
                     /locus_tag="PLI30_05055"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019538087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HOI78958.1"
                     /translation="MNYYSTNQSAPVVSLREAVVKGLAPDKGLYMPESIPHLPEVFFD
                     EIGRFGLITIARTVARAFFGEDIPQTKLDEIVADTLNFDIPLVKVEDGIYALELFHGP
                     TFAFKDVGARFMARIVSYFVQEQRQEEVKVLVATSGDTGSAVANGFLGVKGIRVFVLY
                     PSGKVSDIQEAQFTTLGKNIVALEIDGTFDDCQALVKSAFMDEELNLQMNLTSANSIN
                     VARFLPQSFYYFYAYAQLARQGISTDVILSVPSGNLGNLTAGLFAKRMGLPVKRFIAA
                     NNRNDVFREYLQSGKYTPRPSVQTIANAMDVGSPSNFERILDLYGHSHEAISKDISGY
                     RFTDEEIQSAIKGVYKGSSYLLDPHGACAYLALKKGRKAGEAGIFLATAHPAKFKETV
                     ESCIAKEIEIPEGLGAFMKQKKQSYPLPRNFAAFKKALINLS"
     gene            5616..>8136
                     /locus_tag="PLI30_05060"
     CDS             5616..>8136
                     /locus_tag="PLI30_05060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019538088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative LPS assembly protein LptD"
                     /protein_id="HOI78959.1"
                     /translation="MRLESDWGFSLMKQLITVLYFLTVTTLLPQLSFSQTRTLSSDSL
                     RQQPTDSVTADSLSTKEKLTAPVFYTAQDSMVFTKGNLGYLYGDADVKYGDLGIKGEQ
                     ITMNLDSNLISSTFGLDSVGKEFGFPVFTQGGTEYEMKKVRYNFDTGKAFISNVITQQ
                     GEGNIVANTAKKNADNSFYMVNAKYTTCDLHDHPHFYLNLSKAKVRPEKDVVTGPAWL
                     VVADVPLFPIVLPFAFFPFTETYSSGIIMPSYGDEMERGFYLQNGGYYFAINDYVDLA
                     LTGEIYTKGTWGLSARSNYRKRYKFSGSVNGSYLNTVIGDKGLKDLNIPGAYSVSKDF
                     RINWTHSQDPKANMYRTFSASVNFSTSRANLNDLSRLYSREASNNTKSSSVNITQRFP
                     DSPWSLSASMNINQVSRDSTVSATLPNISITMNRIYPFKRKKMVGAERWYEKISMSYS
                     GEFRNSVNAIKENRFFKSNLIKDWQNGMRHSIPVSATFSLFDVIQISPSVNYTERWYT
                     GGIKEAWDPVEKRNVVVDTVNGFKRVYDYGASISANTKLYGMYVPWKIFGDKVQAIRH
                     VFSPSISLSYKPDFGDPKYGFYEKYSYRNEFGEDVEYSYSPYSRMMFGTAPAGQSGSI
                     GFDFKNNLEMKVKSESDSTGFKKMSLIDDLGVNFSYNMMADSMKWSMINTNVRLKLSK
                     SYTLSLNATWDPYVYELDKNGRPVAVDKLRVFNGKGIGKLQSTGTSFSYSVNQDTFKK
                     LFGKEDASNEDKEGGEDSNANLPDDGTMGRNPNETAAKKSVFDTGSQSIGEFDDDGYL
                     KNQIQWNLSFNYSVRYGYDMQNFDFDRMEYPGKLTHSLGFSG"
BASE COUNT         2330 a         1978 c         1885 g         1943 t
ORIGIN      
        1 caataatctt cccaatattc ctggaggtaa aaaacttata tatacgcatt taacaatgcc
       61 attaacccct atcactgact ttgagaaatt agggaaaacc agtgagctgt tcaaagaact
      121 agccaatata tgtaacaacc ataagggaat gtggagcgta gaagcagaaa attatttgct
      181 agcgaatatt gtaaaatgaa taaaacgaag cgtaaggttc tagtcaataa aattgatggg
      241 aaacgtataa aataaattca ttctttattt ttcaacaccc caaccagcaa ttcaaagctg
      301 gttggggtgt ttgttttatt tttattgttt aacctccatt ttttcagcat ttttatcatt
      361 ttcttaaatt attcctttat ctttgcaata tatttagacg ttgcgatgat tttttcacat
      421 tcaacataca cgactccctt cactatgact atgaccccct ggggggttat ataatatttt
      481 acatccacac ggcaacgctg tttgccacat gcaaatcaac cggtttgcca catgccatcc
      541 tacttttttt tgtttcatta aaaaaccgtt cacaacggta aaaatatatt catacacatg
      601 aaagttatga aatttggagg gacatccgca gggactcccg aaagtatgct tttggttaaa
      661 aacatcatag aagctgaaaa agagcctgtg attgttgtgg tctccgctct tggaggcgtg
      721 accgaccgtc ttctactggc agcagatttt gcgttaaatg caaacccggg ttaccaatcg
      781 atattggaag aaatcatctt ccgacacgag gagatgatcg ggaaaatggc agtttccgct
      841 gtcgatcaaa aggaactgag gcagaaaatc gacccgatgt tcgaagacct gcgcaacata
      901 ttgcggggtg tttacctgat cggagacctg tcgcagaaga catcggacaa gatcgtcagc
      961 tacggcgaac gtttctcctc gctaatcgta agcaaaataa tcgacggggc tagagtttat
     1021 gattccactc agtttatcaa gaccacaaaa caattcagcc atcacatccc cgacatcgcg
     1081 tattccaacg aactgatcaa gcaggcattt ttaagtgacc cgcttcccaa agtggccata
     1141 gttcctggat ttatctcgtc gagcaaggag gcggacgata tcactaacct gggccggggt
     1201 ggatcggact ataccgccgc cattatcgct gctgcactta acgcttctgt gctggaaata
     1261 tggacggatg tggatggctt catgacggcc gaccccaaaa tcatcaactc ggcttacgtc
     1321 attgaagaac tctcgtttac ggaggccatt gagctttcca acttcggggc aaaagtgatc
     1381 tatcccccaa ccatcttccc cgtctaccac aaaagcatac ctatccgcgt aaagaatact
     1441 ttcaaacctg cttcggaggg cacacttatc cgcaatataa agccgacccc taacggaaaa
     1501 ataatcaaag gcatctcgtc gatcaacgat accgccctga ttaccataca gggactcggc
     1561 atggtaggag ttatcggcgt aaacaaacgg atcttcacgg cattggcgga taacggcatc
     1621 agcgtttttc tggtctccca agcatcctcc gaaaacagca catccatcgg agtccgcacc
     1681 caagatgccc cgctttccca aaaagtactc agcaaagaat ttgcgaaaga gatagagagg
     1741 ggaagcatca acgagattat tgtcgaatac gacctggcaa ccattgccgt agtgggacaa
     1801 aacatgaaac acgtgccggg ggttgccggc aaatttttcg gaacgttggg aaggggtggt
     1861 atcagcgttg tcgcgctagc tcagggtgca tcggaaacca atatttcctg cgtgatcgcc
     1921 aaaaccaacc tgaaaaaagc actcaacatc atccacgatt cgttcttctt gtcgccttac
     1981 caggaattaa accttttcgt tatcggaacc ggaacggtcg ggagcaaact gctggcacag
     2041 attaagcagc aaaagcacat tttagaggaa cagaacaagc tgaaaataaa cattgtagga
     2101 attgccaacg gacgaaaagc cttgttctcg cgcgacggga tttccctcga gaattacttc
     2161 gaaaacctga tgtccagggg cgtcaaaagt tcacctcaac tgatccggga tgaaatactc
     2221 aaaatgaata tcttcaattc cgtctttgtg gattgtactg ccagccaggc catttcagaa
     2281 ttgtatgaaa gtctgatatc cagaaatata tcagtggtga cggcaaacaa aattgcagcc
     2341 tcgtccgact acaagaacta ccatcacctg aaggagaccg cccgaaaaac cggaacaaaa
     2401 tttcttttcg aaaccaatgt aggggcggga ttgcccatta tcaacacgat gaactccctc
     2461 accaactcgg gagataaaat cgtcaagctc caagcagtac tatcggggac actgaacttt
     2521 attttcaata ccctgagcga ggatgttcct tttagcaaag ccataaaaat ggcggtggac
     2581 gctcagtttg ccgagcccga cccccgtatc gacctgagcg gattggatgt tacccgcaaa
     2641 ttggtaatcc tttcccggga agccggcgct caagtggaac aggaaaatgt gcaaaagcac
     2701 ctgttcatcc cgcaaaagta tttcgaaagc acccttgatg agttctggtc aaccatcccc
     2761 gaaatggatg aagcttttga aaaccggaga aaaaaactcg ataaagaagg gaaaaagctc
     2821 cgctttattg ctacgtacga caacggcctg tgcgaagtcg gcttacacga ggtggaaaag
     2881 ggacaccctt tctacgacct ggaaggtagc aacaacatca ttatgatcac caccgaacgc
     2941 tacaatgagt atccgatggt catcaaaggc tatggtgcag gggcaagcgt taccgctgct
     3001 ggcgtttttt ccgacattat ttctattgcg aacatacgat aaagaaagaa aaatgaaata
     3061 catcatcatc ctcggagacg gcatggcaga cgaacccgtt gcaaaactcg gcaacaggac
     3121 gcccctacag gcagccaaaa caccgtatat ggataggctg gcacaaaaag gacgcagcgg
     3181 catgctcgac accattcccg aaggcatgtc tccgggaagc gaaattgcta atctttccgt
     3241 gttgggatac gatgttcccc gggtgtacga aggccggggt gtgttggaag ccgccagcat
     3301 gggtgtagat attctgccgg acgaaatggc gatgcggtgt aatttagtga ctatccgggg
     3361 cgatgtgctg aagaatcact ctgccggaca catttccaca gcagaagctg ccgaactgat
     3421 tcattttctg gacgacaggc tcgggaatga ccggtttcgc ttttatcccg gtgtttcgta
     3481 ccgccattta ttaaaaatga aaggtggaaa caaaaacatc gtttgtaccc cgccccacga
     3541 tgttcccgac aagccttttt accccttgct gatcaaaccg gccgaagagc ccgcccaaag
     3601 cactgctgac gaactgaaca gactgatcct tgcctcgcaa gaaatactgc cggctcatcc
     3661 cgtaaatcaa aagaggattg ccctgggaaa agatccggcc aacagcatct ggctgtggtc
     3721 gcccgggtat cgccccggca tgcagccgct gaacaagaaa tatcctattc aaaaaggagc
     3781 ggtaatctcg gccgttgacc tcatcagggg gatcggtgta tatgcaggat tggaggttat
     3841 ccaggtggaa ggtgccaccg ggttgtacga caccaactac gaagggaagg cccgtgccgc
     3901 cctggatgca ctgaaagaga acgattttgt tttcctgcat attgaagcca gcgacgaagc
     3961 cggacacgaa ggggatgtgg atttgaaagt gagaaccatc gaatacctcg attcgcgcat
     4021 cgtaaaaccc attttcgaag aaacctcaac gtgggacgaa cccgtcacta ttgccgtctt
     4081 acccgaccat cccactccct gtgctatccg cacacacacc cgtgatgccg ttccttttgt
     4141 tgtctatcac aaaggaattg aacccgacag cgtaaaaaca tacgatgagt ttgctgcgaa
     4201 aaaaggtgtt tttggccttc ttcgcgggga tgaatttatg aagaatctga ttttgtagaa
     4261 catgaactat tacagcacca accagtccgc acccgttgta tcgctccggg aagccgtggt
     4321 aaaaggactt gctcccgaca aggggcttta tatgcccgaa agtatccccc atcttccgga
     4381 ggtttttttc gatgaaatcg gccgttttgg attgataacc atcgctagaa cggtagcccg
     4441 ggctttcttt ggggaagata tcccacaaac aaaactggat gagatcgttg ccgacacgct
     4501 caattttgac attcctcttg taaaggtgga agacggaatt tatgcgctgg agctttttca
     4561 cgggccaacc tttgccttca aggatgtggg ggcccggttc atggcacgaa tagtatccta
     4621 tttcgtgcag gaacagaggc aggaagaggt aaaagtactg gtcgccactt cgggcgacac
     4681 gggaagcgcg gttgccaacg gatttttggg agtaaagggc atccgcgttt tcgttttgta
     4741 cccatcggga aaggtgagcg atattcagga agctcagttc actaccttgg gaaaaaatat
     4801 tgttgcactc gaaattgacg gaacgttcga cgattgccag gccctggtga aatcggcgtt
     4861 tatggatgaa gaactaaacc tgcagatgaa tctcacctca gccaactcca tcaatgtggc
     4921 gcgcttcctg cctcagtcgt tttattattt ttacgcttat gcacaactgg ccaggcaagg
     4981 gatttctacg gatgtgatcc tgtctgtgcc cagcgggaac ctgggcaacc ttaccgcggg
     5041 gcttttcgcc aaacggatgg gattaccggt aaaacggttt attgccgcta acaaccgcaa
     5101 cgatgtattt cgggaatacc tacagtcggg gaaatatact ccccggccct ctgtacaaac
     5161 catagccaac gccatggacg taggttcacc cagcaacttc gagcgcatcc tcgaccttta
     5221 cggacactct cacgaagcca tttcaaaaga tatttccgga taccggttta ccgacgaaga
     5281 gatacaatcg gctatcaaag gggtatacaa aggatcaagc tatctccttg acccacacgg
     5341 cgcctgtgct tacctggccc tgaagaaagg ccggaaagcc ggtgaggcag gtattttcct
     5401 cgccactgca caccctgcca aatttaagga aaccgtggag agttgtatcg caaaggaaat
     5461 tgaaattccc gaaggattag gggcattcat gaaacaaaaa aaacaatcgt atccgctccc
     5521 ccggaatttc gctgcgttta aaaaagcgtt aataaacttg tcttaagttt tattttggat
     5581 attttttaac gctattaaac ctaaattgat tagttttgcg cttagaatca gattggggct
     5641 tttcgttgat gaaacaactc ataaccgttt tatattttct aaccgtaacc acgcttcttc
     5701 ctcaactttc tttttcccaa acaagaacgc tgagcagtga ttcattgcgt caacagccca
     5761 cagactcagt tactgcagac tcactatcga caaaagagaa gctcacggcc ccggtattct
     5821 atacagccca ggattccatg gttttcacca aaggcaattt gggttacctt tacggtgacg
     5881 ccgatgtaaa atacggcgac ctgggcatca agggggaaca gatcaccatg aacctggata
     5941 gcaatctcat ctcatccacc ttcgggctcg attcagtcgg aaaggagttc gggtttcccg
     6001 tatttacgca gggaggtacc gaatacgaaa tgaaaaaggt ccgctacaat ttcgacactg
     6061 gcaaggcatt tatatccaac gttataacgc aacagggaga agggaacatc gtcgccaaca
     6121 cggcgaaaaa gaatgccgac aattccttct atatggtgaa tgccaaatac accacctgcg
     6181 acctgcacga tcatccgcat ttttacctga acttgtcgaa agccaaggta cgtcccgaaa
     6241 aagatgttgt cacaggcccg gcctggctgg tggttgccga tgtcccgctt ttccctattg
     6301 tcctgccctt tgcttttttc cctttcaccg aaacctattc ttcgggaata attatgccat
     6361 cgtatggtga tgagatggaa cgcggatttt atcttcagaa cggcggatat tactttgcca
     6421 ttaacgatta tgttgattta gcgctgaccg gcgagatata taccaaaggg acctgggggc
     6481 ttagtgcacg gtctaattac cgcaagaggt acaagttttc cggtagtgta aacggaagct
     6541 acctgaatac cgtcatcgga gataagggat taaaagacct gaatattccc ggtgcctatt
     6601 cggtctccaa agatttcagg attaactgga cccactcgca agacccgaaa gccaacatgt
     6661 accggacgtt ttccgccagt gtgaacttct caacaagccg tgccaacctg aacgacttat
     6721 cccggctcta ctcccgcgag gcatccaaca acaccaaaag ttcgagtgta aacatcacgc
     6781 agcgcttccc cgattcgccc tggagtttat cggcatcgat gaatatcaat caggtctcgc
     6841 gcgactctac tgtctctgca acgttgccca atatctccat caccatgaac cgtatctacc
     6901 cctttaaacg taaaaaaatg gtaggtgccg aaaggtggta cgaaaaaata tccatgagct
     6961 attcgggtga attccgcaac tcggtaaacg cgatcaagga gaacaggttc ttcaaatcaa
     7021 accttatcaa agactggcaa aacggcatgc gccacagcat cccggtgagt gccacctttt
     7081 cactgttcga cgtgatccag atatctccgt cggtgaacta caccgaacgg tggtacaccg
     7141 gcggaataaa agaggcatgg gacccggtgg aaaagagaaa cgtggttgtg gataccgtaa
     7201 acggattcaa aagggtgtac gactatggcg cctccatcag tgccaataca aaactgtacg
     7261 gaatgtacgt tccctggaag attttcggag ataaagttca agccatccgt cacgtctttt
     7321 cacccagtat cagcctgagt tacaaacccg attttggaga tccaaagtac ggtttctacg
     7381 aaaaatactc ttaccgcaat gaattcgggg aggatgtgga atattcttat tcgccttatt
     7441 cccgaatgat gttcggaacc gctcctgcag gacaaagcgg gagtatcgga ttcgatttta
     7501 agaacaacct cgagatgaaa gtgaaaagcg aaagcgacag taccggattc aagaagatga
     7561 gcctgatcga tgacctgggt gtaaacttca gctacaacat gatggcagac tccatgaaat
     7621 ggagcatgat caacaccaat gtccgtttga aattgtcaaa atcctacacc ctgagcctta
     7681 acgctacttg ggacccgtac gtttatgagc tggacaagaa cggaaggccg gttgcagtgg
     7741 acaaactacg tgtttttaac ggcaaaggaa tcggaaaact gcaaagtacc ggcacctcgt
     7801 tctcctactc cgtcaaccag gataccttta agaaactgtt cggcaaagaa gacgctagca
     7861 acgaggacaa ggagggcgga gaagactcca acgccaacct tcctgatgac ggcacgatgg
     7921 gacggaatcc aaacgaaacc gccgcaaaga aaagtgtttt tgataccgga agccagtcca
     7981 taggagaatt tgacgatgac ggctacctga aaaatcagat tcagtggaat ctctcgttta
     8041 actattcggt aaggtatggg tacgatatgc agaatttcga ttttgaccgg atggaatatc
     8101 cgggaaagtt gacgcacagc ctaggtttca gcggtt
//
LOCUS       DAOSDR010000091         8132 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrimonas sp. isolate AMR_MDS_1826
            NODE_3282_length_8132_cov_3.495110, whole genome shotgun sequence.
ACCESSION   DAOSDR010000091 DAOSDR010000000
VERSION     DAOSDR010000091.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863355
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrimonas sp. (wastewater metagenome)
  ORGANISM  Petrimonas sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Dysgonomonadaceae; Petrimonas.
REFERENCE   1  (bases 1 to 8132)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8132)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:10:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,720
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 35
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8132
                     /organism="Petrimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3282_length_8132_cov_3.495110"
                     /isolate="AMR_MDS_1826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2023866"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            227..784
                     /locus_tag="PLI30_05065"
     CDS             227..784
                     /locus_tag="PLI30_05065"
                     /inference="COORDINATES: protein motif:HMM:NF019265.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma-70 factor"
                     /protein_id="HOI78960.1"
                     /translation="MAALKKGDPHAFDELFHLYGKRLYHFSLGYLKSKPEAEEVVQEV
                     FLKIWNNRESLKTECSFQAYLFTIAYRHIRDLFLKISREQAYRRQIVEASLHFNDELD
                     ERTNYQSLLELVDQLIDELPPRQKEIVFLRKKEGIPVKEIAGKLGISPKTVENHLTEA
                     MKSLRRGLDNESGAGLLFFLLFLKN"
     gene            983..2020
                     /locus_tag="PLI30_05070"
     CDS             983..2020
                     /locus_tag="PLI30_05070"
                     /inference="COORDINATES: protein motif:HMM:NF016648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4974 domain-containing protein"
                     /protein_id="HOI78961.1"
                     /translation="MTARTDILKRFFSGNYSRKDYRLLLKELEKGEDIRQAMEQHWLE
                     WGHDELPEGDVDYLLDRIQHRIKLDENSSRKIKSGWELFRRTAAILIVPLALSFLVYW
                     WLHRDSSTVGNAYAEIQCPLGVRTKFELPDGTTGFLNSGSTLKFPVRFGTERQVKLAG
                     EAYFDVKQRKESPFYVHTENLDIKVLGTTFNVVAYPEEPTEEVTLLRGAVDISQNNGK
                     HLATLTPDRQITFDTNRQRIVNKEVIATQYTSWTEGKLVFRNENMEQVAQRLSRWYNA
                     EIVVAAPQLKKYTFHATFADEQLDEVLKLLALTTPISYTEAERVSSPDGVYRKRQIIL
                     KINPEKLKEFK"
     gene            2171..5461
                     /locus_tag="PLI30_05075"
     CDS             2171..5461
                     /locus_tag="PLI30_05075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005633403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOI78962.1"
                     /translation="MRLSVFFLLVLAAQAWATTTYSQQTRLSLNMKGSKVIDVLNEIE
                     DKSEFYFLFNQKLVDVERKVDIEVKQENIEAILSRLFDHTNVSYLVIDRQIVLTTAQA
                     DAVAVQQHKTVTGKVTDQADQPLPGVTVLLKGTTNGTVTDVSGNYSLSNIPAEATLVF
                     SFVGMKMQEIAVEGKASINVKLVEDAIGIEEVVAIGYGTMKKSDITGSVVRADLTKLQ
                     ESPNISLGQSLQGTIPGLNVGAVNTAGSDPSITIRGRTSISGGQSPLIVLDGIIYRGS
                     LVDINPNDIESIDVLKDASAAAIYGSQASNGVILITSKTVKTMSKPVIEYTGSYTIQE
                     STNNKMRPMDKEGFLQLIADRWLELSRTGPDLLQPNPTWDVTKFLMDANAVNGYIAGV
                     ETDWWEMGTNDHPYIQMHNISVRGRSELSNYFISVGLTDQENLVINDTYKRYNIRLNL
                     DTKITDWLKVGAQTFFTLSDWSGIASMGSTLPPVVPSVDEETGNYIIYPYKGTLNPAL
                     ARQQDNLDKRYNFFGNFYTDMDIPFVKGLNYRLNFSQNLIMDKDFTFNPWGAELLGSG
                     NKQNDSQYSWTADHILSYKQSFGKHDVHSTLVYGAEEREWERTNASGSDFSNDALGYN
                     YLGAANAERQVISSDAWKETSLYTMLRLAYTYNSRYSFTGTIRRDGFSGFGKNNKFAW
                     FPSVAAAWRISEENFIKDNLEWVDNLKLRISYGSNGNRTVGRYQTLAKLSSMDAYLYG
                     DGASAEKGVYLNAMANDNLKWETTNAFNVGLEYNILNNRIFGNIEYYRSNTFDLLYNI
                     NIPVLNNNISSIPTNIGKLANRGAELTITGIPVQTKNFGWDITVNFSQNRNKVISILG
                     IDHDGDGKEDDLVSSKIFIGHPYGVAYDFEQIGMWQIADHQAGIIPQGFTYGTYKVRD
                     ISGPDGKPDGAYTAAYDRTILGYTDPSYRFSIENVFHYQNWELKAFINSIQGGKDYYY
                     AQPASGLSNPDNIYQNNSFKWDYWTPENPNARYRQPGYFTAALGNAYSPYIQRSFVRL
                     QNLTLSYRLPSELLKKAGVNNFKIHLTGTNLFTITDWDGWDPETSTGLGGDYPLLRTY
                     SLGINFDF"
     gene            5474..7180
                     /locus_tag="PLI30_05080"
     CDS             5474..7180
                     /locus_tag="PLI30_05080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005644885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HOI78963.1"
                     /translation="MKKTFKITGLLSLITLFTLMSCNENEFLTEEPLAIYTAENSLVT
                     ASDFQAAVNMLYRRPGDIFIYSTDDGFFSLYHGTDLGYCTAAFDRLNTYAATMVPENA
                     LLNHVWNNSFFMVNQANLILTRISNADIPEVDKNVLRGEALFFRAFACRILANLFGGV
                     PLYSEEITAPRRDFVRSSRTETYTQAKKDLEEAVSLLKNIDAIKDGKISKQMAQHLLS
                     EIYIALGEFDNAITAASAVIDYPAMELMTERFGSKLEQPGDVYSDLFKTGNQNRSSGN
                     KESLWIMQYDYLNPASMRNDSWPWKFNPFYQNINVTAGGKTTTAFRGVTAEKGGRSIA
                     WMHPTDYVMFGIWEDDPNDMRISEHNIIRDFKIDNPASLAFGKWFVADGYSEKATARQ
                     WFPFVMKKVMGDIPEDYWQRDANGNPLLTAFGEHLVTNNGNSTFRDMYMFRLAETYLL
                     RAEAYVGKNNKVAAAADINVIRARANAKPAVSSEMDLDYILDERLRELYYEEPRMLTL
                     CRTEKLAERNRKYNYSYTDITGTYECAGTSIQDYHNLWPIPYSEIERNTEAVLEQNPG
                     YN"
     gene            7653..>8132
                     /locus_tag="PLI30_05085"
     CDS             7653..>8132
                     /locus_tag="PLI30_05085"
                     /inference="COORDINATES: protein motif:HMM:NF025851.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase N-terminal domain-containing
                     protein"
                     /protein_id="HOI78964.1"
                     /translation="MKTIAPIILSFLLSHLILSCSSPEHQEVKLWYDQPAEKWADAFP
                     LGNGRMAAMCYGGTDTSRFQLNEESLWAGTQINPVPENYFKNLKKIQKLVMAGKLAEA
                     HDFGVEKLTVRPTSFRSYEPLCDLLVDLDLAGPISNYRRELDMVSGISRVEYLSGETK
                     "
BASE COUNT         2488 a         1733 c         1813 g         2098 t
ORIGIN      
        1 tgaaggcacc ggacgcggct actcttcgct gacggcagtg gacgaacaaa ccatcggaat
       61 cctgtacgaa agcagtcagg ccgacatggt ttttcaaaaa atcagcctgc acgaaatcct
      121 gaaagaaggg cacaccccgt tctgaatatt gtttattatt acttaatttg actttcgctc
      181 atttttttag ttggaaacta aatcagaaaa tatcgacaaa caatgggtgg cagccttaaa
      241 aaaaggcgac ccgcacgctt ttgacgaact gtttcacctg tacgggaagc ggctctacca
      301 tttttcactg gggtacctta aatcgaaacc tgaagcggaa gaggtggttc aggaagtgtt
      361 tctgaagatc tggaacaacc gcgaatccct caaaacagag tgctcttttc aggcctatct
      421 tttcacaatt gcttaccgcc acattcgcga cctcttcctg aagatcagtc gtgaacaagc
      481 ctaccgtcgt caaatcgtgg aggcttcgct ccattttaac gacgaactgg acgaacgtac
      541 caattaccag tcgttgctgg aactggttga ccaactcatt gacgaacttc ctccccgtca
      601 aaaagagatt gtgttcctgc gaaaaaaaga ggggataccg gtgaaagaaa ttgccggaaa
      661 actgggtatt tctcccaaaa cggttgagaa ccacctgacc gaagcgatga agtcactcag
      721 gagaggtctg gataatgaaa gtggggccgg cctactcttt tttctgctgt tcctgaaaaa
      781 ttagtacgaa aaaaaccggc tgcacacagt tgcctgacac cccctcaaaa tagcggcctg
      841 accacccgtg atttttattt tttgtttcaa ctaggggaat tggaattctc aaccctatat
      901 atcaataaag gcggacttca tcttacgatc cgactttgga cacaaatttt aattgttttt
      961 tcaactttct gaaaaaaagc tgatgacagc acgcacagat atattgaaac gttttttctc
     1021 cggaaactat tctcggaagg attatagact cctcctgaaa gagctcgaaa aaggggagga
     1081 tatccgtcag gcgatggagc aacactggct tgaatggggg cacgacgaac tgccggaagg
     1141 agatgtggac taccttctcg acagaataca acaccgcatc aaacttgatg agaacagcag
     1201 tcgaaaaata aaatcaggct gggagctttt caggcgcaca gcagctattt tgattgtccc
     1261 gctggcactc tccttcctcg tttactggtg gcttcaccgc gacagctcta cggttggcaa
     1321 cgcctatgcc gaaatccagt gcccactggg ggtacgcacc aagtttgaac tccccgacgg
     1381 taccaccggc tttctcaaca gtggctcaac cctgaaattc cccgtccgct ttgggacaga
     1441 acggcaggta aaattggcag gggaagccta cttcgatgta aaacagcgga aggagtcccc
     1501 tttttatgta catactgaaa atctcgacat caaagtatta gggactacct ttaatgtggt
     1561 agcctatccg gaagagccaa ccgaagaggt aaccctgtta cgcggcgccg ttgacatttc
     1621 acaaaacaac gggaaacacc tcgcaacact cactcccgac cggcagataa cttttgatac
     1681 caaccggcaa agaattgtca acaaagaggt tatagcaacc cagtacacct cctggaccga
     1741 aggcaaactg gtattccgga atgaaaacat ggaacaggtt gcccaaaggt taagccggtg
     1801 gtacaatgct gaaatcgtag tggcagcccc tcaactgaaa aaatacacct ttcacgccac
     1861 ttttgctgac gaacagttgg atgaggttct gaaactcctt gccctgacaa cgcctatctc
     1921 ttacacagag gcagaaaggg taagcagccc cgacggggta taccggaaac gacaaataat
     1981 cttaaaaatc aatcctgaaa aactgaaaga atttaaataa aaaacaggag gtgttggcac
     2041 cacgctcctg ttgtatcaag cagtgttaca cgtatttaat tgtttaacta cttaatgttc
     2101 caaatttatg aaaaaaaaac ggatttgtgg ggcggagcca tttccgaacc taactaaact
     2161 atggaaaatt atgcggctaa gtgtattttt tcttttggtg ttggcagccc aggcctgggc
     2221 cacgacaacc tattcgcagc aaacgcgcct ttcactgaat atgaaaggaa gcaaggtaat
     2281 tgatgtgctg aacgaaatcg aagacaaaag cgagttttat ttcctcttta accaaaagct
     2341 ggttgacgtg gagcgcaagg tggacatcga agtaaagcag gaaaacatcg aagccattct
     2401 ttcgaggctg tttgaccaca ccaacgtaag ctacctggtc atagaccgcc aaattgtgct
     2461 gaccactgcc caggcagacg cagtggctgt gcaacagcac aaaaccgtta cgggtaaagt
     2521 caccgaccaa gctgaccaac cattacctgg agtaactgtg ttgcttaaag gaactactaa
     2581 tgggacggtt accgatgtga gcggaaacta ctccttaagc aatatccctg cagaagccac
     2641 gctggtgttt tcgtttgtcg ggatgaagat gcaggaaatt gccgttgaag gaaaagcttc
     2701 aatcaatgta aaattggtgg aagatgccat cggcattgaa gaggtcgtgg caattggtta
     2761 cggaaccatg aaaaaaagtg atataacggg ttccgttgtt cgtgctgatt taaccaaact
     2821 acaggaatct ccgaatatca gtttaggaca atcgttgcag ggaaccatcc ccggactgaa
     2881 tgtgggtgct gtaaatacag caggcagcga tccttccata actattcgcg ggcgtacttc
     2941 tatatccgga gggcaaagtc cgttgattgt attggatggt atcatctatc gggggagtct
     3001 agtggatata aacccaaatg acattgaatc aatagatgtt ttaaaggatg ccagtgctgc
     3061 agcaatttac ggctcacaag ctagtaatgg tgtcattctt attacttcca agacagtcaa
     3121 aaccatgagt aaacctgtta ttgaatatac tggttcttac accatacagg aatctaccaa
     3181 caacaaaatg cggccaatgg ataaggaagg ttttttgcaa ctgattgctg acaggtggct
     3241 tgaattgagt cgcacaggtc cagacttatt acagcccaac ccaacgtggg acgttactaa
     3301 attcctcatg gacgcgaatg cagtgaatgg ttacattgct ggtgtggaaa ccgactggtg
     3361 ggaaatgggt acaaacgatc atccatacat tcaaatgcac aacatcagcg tcagggggcg
     3421 gagcgaactg agtaattatt tcatatctgt cgggttgact gaccaggaaa atctggtgat
     3481 caatgatacc tacaaacgat ataacatccg tctcaacctc gacacgaaaa tcaccgactg
     3541 gctcaaagta ggtgctcaga ctttttttac cctgagcgac tggtcgggta ttgcaagtat
     3601 gggtagcacc ctacctccgg ttgttccttc cgtggatgag gaaacaggga attatatcat
     3661 ttatccgtat aagggtacgt tgaatccggc actagcacgt cagcaggaca atctcgacaa
     3721 gcgttacaat ttcttcggta atttctatac cgatatggat attccttttg taaaaggact
     3781 gaactatcga ctgaactttt ctcaaaacct gataatggac aaggatttta cattcaatcc
     3841 atggggtgca gaactgcttg gttcgggaaa caaacagaac gacagccagt acagttggac
     3901 ggctgaccat atcctgtcct ataagcagtc attcgggaaa catgatgtcc attccacatt
     3961 ggtttacggt gctgaagaaa gggaatggga acgtacaaac gcctctggtt cggatttttc
     4021 aaacgatgca ttgggataca attatcttgg ggcagcgaat gctgaaagac aagttatttc
     4081 ttctgatgca tggaaggaaa cgagccttta taccatgctg cgccttgctt acacttacaa
     4141 cagccgctac agctttacgg gaacaattcg tcgcgacgga ttctcaggtt ttggaaaaaa
     4201 caacaagttc gcctggtttc cttctgtagc cgcagcatgg cgcattagcg aagaaaattt
     4261 tataaaagac aatctggaat gggtggataa cctgaaactt aggatctcct acggttcaaa
     4321 tggtaaccgt actgtagggc gttatcagac tttggccaag ttgagttcaa tggatgctta
     4381 tttgtatgga gatggagcat ctgccgaaaa gggtgtatac ctcaatgcca tggcgaacga
     4441 caatctgaaa tgggaaacta caaatgcatt taatgttggc ttggaataca acatattgaa
     4501 caacagaatt tttggcaata tcgaatatta ccggtcaaat acatttgatt tactttacaa
     4561 cataaacatc ccagtactaa acaataacat cagctcgatt ccgaccaaca tcggaaaact
     4621 ggcgaatcgc ggtgctgaac tcacaatcac cggaattcct gtccaaacaa aaaattttgg
     4681 ctgggatatt acagtaaatt tctctcaaaa ccggaataaa gtgatcagta ttcttggtat
     4741 tgaccatgat ggcgacggca aagaggatga cctcgtgtca agtaaaattt tcattggtca
     4801 tccttatgga gtagcctatg attttgaaca gatcggaatg tggcaaatag cggatcacca
     4861 ggcaggtatt ataccacaag gtttcaccta cggaacgtat aaagttcggg atatcagcgg
     4921 acctgacgga aagcctgacg gcgcctacac agctgcttac gaccgtacaa ttcttggcta
     4981 tactgatcca tcgtaccgtt tcagtataga aaacgtattt cattatcaga attgggaact
     5041 gaaggcgttc atcaattcta tccagggcgg aaaggattat tattacgcac aacctgccag
     5101 tggactgtct aatccagata atatttatca gaacaatagt tttaaatggg attactggac
     5161 accggaaaat ccgaatgcac gttacagaca gcccggatat tttacggcag cgttgggtaa
     5221 tgcatattcg ccgtatattc agagaagttt tgtgcgtcta caaaatctta ccttatcata
     5281 ccgcttacca tctgaacttt tgaaaaaagc tggtgtgaac aacttcaaga ttcacttaac
     5341 aggcaccaat ctctttacca ttaccgattg ggatggatgg gacccggaaa caagtacagg
     5401 attgggagga gattacccac ttttgagaac atattcatta ggcattaact ttgactttta
     5461 aaatcgaaga gacatgaaaa aaacatttaa aataacggga ttattgagtt tgataacttt
     5521 atttaccctc atgtcctgca acgaaaacga atttctgaca gaagaaccac tggctattta
     5581 tacagcagaa aattcgttag ttacggcctc cgattttcag gcggcagtaa atatgttata
     5641 tcgtagacct ggtgatatat ttatttattc cacagatgat ggctttttct cactttatca
     5701 tggcaccgat ctgggatatt gcacagctgc tttcgatagg ctcaacacat atgctgccac
     5761 aatggttccc gaaaatgcat tattaaatca tgtatggaac aactcgtttt ttatggttaa
     5821 tcaggcaaac ttaattctaa cgcgcatcag caatgctgac atcccggaag tcgacaagaa
     5881 tgtactacgg ggagaagcgc tatttttccg ggcattcgca tgccgtatcc tggctaattt
     5941 gtttggtgga gttcctttat attcggaaga aatcaccgca cctcgccgcg attttgtacg
     6001 ttctagccga acagaaacct acacacaagc gaaaaaagat ctggaggaag cagtatcatt
     6061 actgaaaaat attgatgcga tcaaagacgg taaaatttcg aaacagatgg cgcaacacct
     6121 gctttcggag atctatattg cactgggtga gtttgataat gcaatcacag cagcatcagc
     6181 agtaattgac tatcctgcaa tggaactgat gacagaacgc tttggttcca aacttgagca
     6241 gcccggcgat gtttattcag acttattcaa aacaggtaac caaaacagga gctcgggaaa
     6301 caaggaatca ctctggataa tgcagtacga ttatcttaat ccggcgtcta tgagaaatga
     6361 tagttggcca tggaaattta atccttttta tcaaaatatc aatgttactg cgggtgggaa
     6421 aacaactacg gctttcaggg gcgtcacggc tgaaaaagga ggccgcagta tagcatggat
     6481 gcacccgaca gactatgtga tgtttggaat atgggaagac gacccgaatg acatgcgtat
     6541 atcggaacat aacatcatac gcgattttaa aatagataat ccggcctcgc ttgctttcgg
     6601 gaaatggttt gttgccgatg gatattcaga aaaagcaaca gctcggcaat ggttcccgtt
     6661 tgtgatgaaa aaagtaatgg gtgatattcc ggaggattac tggcagaggg atgcgaatgg
     6721 aaatcctttg ctgaccgcat tcggtgaaca tttggtgact aataacggga acagcacatt
     6781 tcgcgacatg tacatgtttc gtctggcaga gacatatttg ttacgcgcag aagcctatgt
     6841 cgggaagaat aacaaagtgg ctgccgcagc cgacatcaat gttatccggg cacgtgcaaa
     6901 tgccaaaccg gcagtatcct cagaaatgga tcttgattat attctcgacg aacgtttacg
     6961 ggaactgtat tatgaagaac cacgtatgct gacgctttgc cgtaccgaaa aactggcaga
     7021 acgtaaccga aaatataatt attcatatac cgacataact ggaacttatg agtgcgcagg
     7081 aacatccatt caagattacc ataacctgtg gcctattcct tattctgaaa tcgaacgaaa
     7141 caccgaagct gttctcgaac aaaatccggg atataactaa tactttaatg ggaagctgat
     7201 ctcaaatagt tggatcagct tcccattttt tggaatgaaa aatatattcc gaacgaataa
     7261 tttcttttca cggatttgac tggatatgtt ttttccccga gggagtaaag gatccctacc
     7321 acctaaggca taatcatacc ccgattttgc cggaattact taaagaaaca aatatcaagt
     7381 ctgcttgcgt gtaatgatca gatgaacgaa ttaacgttaa aaaaaaggta attacaaaaa
     7441 aactgttcag gacttctgtc atcaacctga agaagcctct tatagtaaaa cagtttaaaa
     7501 accgatgttt atataaaaga aaaatggata aataattatt ccatactgtt gaaaatcagg
     7561 ttgaaaaaca agcgtaatta tgggatttat agatcgtaga ttgaaaattt gtgcgtggaa
     7621 ataatttgaa taatttttaa aaatctcact aaatgaaaac tattgcacca ataattctct
     7681 cttttctgct aagtcatctc attttgtcat gttcctcacc ggaacaccag gaggtcaagc
     7741 tttggtatga tcagcctgca gaaaaatggg ccgacgcatt tccgcttggt aatggccgga
     7801 tggcggccat gtgttacggc ggtaccgata ccagccgttt tcagctgaat gaggaaagtt
     7861 tatgggccgg aacccagatt aatccggtac cggaaaacta tttcaaaaac ctcaaaaaga
     7921 tccaaaaact ggttatggca ggaaaacttg ccgaagctca tgattttggt gtggaaaaac
     7981 taacggttcg ccctacatct ttcaggtcgt atgaaccctt atgtgacctc ttggtcgatt
     8041 tggatttggc tggcccgatt tcgaattacc ggagagaact ggacatggta tctggaattt
     8101 cgcgtgttga gtatctttcc ggagaaacaa aa
//
LOCUS       DAOSDR010000092         8102 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrimonas sp. isolate AMR_MDS_1826
            NODE_3292_length_8102_cov_3.735678, whole genome shotgun sequence.
ACCESSION   DAOSDR010000092 DAOSDR010000000
VERSION     DAOSDR010000092.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863355
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrimonas sp. (wastewater metagenome)
  ORGANISM  Petrimonas sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Dysgonomonadaceae; Petrimonas.
REFERENCE   1  (bases 1 to 8102)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8102)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:10:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,720
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 35
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8102
                     /organism="Petrimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3292_length_8102_cov_3.735678"
                     /isolate="AMR_MDS_1826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2023866"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..213)
                     /locus_tag="PLI30_05090"
     CDS             complement(<1..213)
                     /locus_tag="PLI30_05090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019538798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5698 domain-containing protein"
                     /protein_id="HOI78965.1"
                     /translation="MFDFLDVYPWLLPVMIFFGRIFDVSLGTLRIIFVSKGEKYIAPA
                     IGFFEVFIWVVIISQILTRANDLVAYL"
     gene            complement(274..1296)
                     /locus_tag="PLI30_05095"
     CDS             complement(274..1296)
                     /locus_tag="PLI30_05095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019538799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HOI78966.1"
                     /translation="MRSFGSDNNSGVHPLMLQAIIDANRNHTIGYGDDDWTREAEKAV
                     KRLIGKEDIEPLFVFNGTGANITALQACTLPFHHIICASTAHIAVDECGAPTKFTGCA
                     LKEIVTSDGKLTPEMVRPLLHGFGVEHHSQPKVIAISQTTEMGTAYTPREIKLLADLA
                     HEHGMYLFVDGTRMANACAFLNVSVKEMTVDCGVDIFTFGGTKNGLMVGEVLVPLRKE
                     LAENIRYYRKQASQLYSKMRFISAQFIPYFNEGIWLENAQNSNAAAQKLAAGMRKAGV
                     KLTQEVQSNAVFFTLSEEETTQLRERYFFYDWDVEQNERRLVCSWDTTEEDLAGFVSY
                     LRTIVK"
     gene            complement(1380..2744)
                     /locus_tag="PLI30_05100"
     CDS             complement(1380..2744)
                     /locus_tag="PLI30_05100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019539216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel"
                     /protein_id="HOI78967.1"
                     /translation="MIQDEMEQLKDNVASDRVESFSQQVTNLISGWSKDFLHGTGVPD
                     TYIGVINSIFLSVILVVLVYVMQTMLRKLITVILNKAVKINRLTISRHLINNKFPKYL
                     AMIIPISIIKGAIPIIFNDFPVAMRLSLKVFDVFFVFYFMWLSVSVINAFTDTLKTKD
                     NFKDKPVESFGQLIRIFVYAIGAIVIISLFIGKTPTTILAGLGAASAILLLIFKDTIL
                     GLVASIQVSSNDMVRIGDWITMPKYGVDGDVIKINLTTVKILNFDKTITTIPPYSLVT
                     EAFQNWRGMTEAGGRRIKRAINIKQSTIHYVNEEELEHFRKIQLLSGYIDERKQIIDS
                     FNNATGADRSLPLNGRNFTNMGLFRKYVELYLKNHPQVHRELLLLVRQLAPTAQGLPL
                     ELYLFTATTSWTEYENIMSDIFDHVTAAVSYFGLEIFEDVSNPLRMSSAKTDTEKMPV
                     PVKK"
     gene            3108..4310
                     /locus_tag="PLI30_05105"
     CDS             3108..4310
                     /locus_tag="PLI30_05105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018965567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase family protein"
                     /protein_id="HOI78968.1"
                     /translation="MGKVLIIGAGGVGTVVANKVAQNSRIFTDIMLASRTKSKCDAIA
                     EDVKRRTGVTIKTAKVDADSVPELVTLFNEFKPELVINVALPYQDLTIMDACLACGVN
                     YLDTANYEPKDVAKFEYKWQWAYRDKFREAGLTAILGCGFDPGVTSIFTAYAAKHHFD
                     EIHYLDIVDCNAGDHGKAFATNFNPEINIREVTQKGKYWENGKWVETEPHEIHQPLNY
                     PNIGPKESYVIYHEELESLVKNFPTLKRARFWMTFGQEYLTHLRVIQNIGMARIDEVE
                     YNGQKIVPIQFLKAVLPDPGELGENYTGETSIGCRIRGIKDGKERTYYIYNNCSHQAA
                     YEETGAQGVSYTTGVPATIGAMMFMQGLWKKPGVFNVEEFNPDPFMEQLNKQGLPWHE
                     LFDVDLEV"
     gene            4329..5246
                     /gene="pyrB"
                     /locus_tag="PLI30_05110"
     CDS             4329..5246
                     /gene="pyrB"
                     /locus_tag="PLI30_05110"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006842285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HOI78969.1"
                     /translation="MKAKSLVNISDYGKEDILRILKAATAFKANPNRDLLQGKVCATL
                     FFEPSTRTRLSFETAVNRLRGRIVGFSDAATSSSTKGESLKDTIMMVGNYADLIIMRH
                     HLEGSARYASEVSPVPIINAGDGSNQHPTQTLLDLFTIYETQGTMENLTITIVGDLKY
                     GRAVHSLIQGLSHFHPSFNFVAPEELKIPEKYKVFCDNLNIPYREYTEFSSEAIASAD
                     ILYMTRVQRERFTDLMEYEKVKNIYVLHNEMLNNSRDNLKVLHPLPRVKEINQDVDEN
                     PKAYYFQQAENGVYTRQAVICDLLDIRLD"
     gene            5278..5730
                     /gene="pyrI"
                     /locus_tag="PLI30_05115"
     CDS             5278..5730
                     /gene="pyrI"
                     /locus_tag="PLI30_05115"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019539212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase regulatory
                     subunit"
                     /protein_id="HOI78970.1"
                     /translation="MGKELQVAALENGTAIDHIPTRELFKVVALLQLQKLDNRITIGN
                     NLKSSKMGAKGIIKVSNKFFKEDEINRIALVAPNVNLNIIRDYEVIEKKKVTLPDEII
                     EIVKCNNPKCITNNEPMKTRFEVVDKENVVLQCHYCELKIKKEEIVLK"
     gene            5753..6319
                     /locus_tag="PLI30_05120"
     CDS             5753..6319
                     /locus_tag="PLI30_05120"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005862715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HOI78971.1"
                     /translation="MKQNWKPGTLIYPLPAAMISCGSHPDEYNIITVSWLGTLCTNPP
                     MCYISIRPERHSYEIIKRNMEFVINLTTEELAQATDWCGVRSGKDYNKFHETGLTPAK
                     AVAVNAPVIEESPLCIECRVKEIITLGSHDMFIADVVNVQADDKYFDAETGKFDMQNA
                     RLLAYSHGNYYGLGEHIGKFGWSVKKKK"
     gene            6339..7277
                     /gene="metA"
                     /locus_tag="PLI30_05125"
     CDS             6339..7277
                     /gene="metA"
                     /locus_tag="PLI30_05125"
                     /EC_number="2.3.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010800193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008899 - homoserine O-succinyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-succinyltransferase"
                     /protein_id="HOI78972.1"
                     /translation="MPVNLPDKLPAIEILSKEHIFVMSDLRASTQDIRPLKILILNLM
                     PLKITTETDLIRLLSNSPLQLEIEFLGLKSHTPKNTPIEHLQTFYKTFPKVKESYYDG
                     FIVTGAPVELLPFEEVRYWQELTGIFDWARTHVTSTYYICWAGQAAMYHFYGIRKYPL
                     DKKIFGVFPHTVNDKAFPLFRGFDDEFFAPHSRHTTVSAEEISQHPELTILSQSEEAG
                     VYIVASRGGRELYVTGHSEYSPLTLHNEYTRDMQKGLDSVELPKNYYKDNDPQKQPVV
                     LWRSHANLLFINWLNYFVYQATPFDLKKIKELGDLT"
     gene            7300..>8102
                     /locus_tag="PLI30_05130"
     CDS             7300..>8102
                     /locus_tag="PLI30_05130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007478833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase U32 family protein"
                     /protein_id="HOI78973.1"
                     /translation="MKAPRIVELLAPAKNKEIGKEAILHGADAVYIGISGFSARMAAG
                     NSIEDIAELVEFAHQYNAKVYVALNTILYDHELLQVEKLIRELYRIHADAVIVQDMGI
                     LQLNLPPIPLHASTQTDNRTVEKVQFLENAGFTQVVLARELSRDQIAEISSQTSIALE
                     VFVHGALCVSYSGQCYISQAITGRSANRGECAQICRLPFDLQDADGRIVRKNAHLLSL
                     KDFNQYDNLEELLDAGVSSLKIEGRLKDVTYVKNVVAAYRQRLDSIFRKR"
BASE COUNT         2381 a         1906 c         1822 g         1993 t
ORIGIN      
        1 caggtaagcc accaggtcat tggcacgggt aagtatctgc gatatgataa ccacccagat
       61 aaacacttcg aaaaaaccga tggcaggggc tatgtatttt tcacctttcg atacaaaaat
      121 gatgcgtaac gtacccagcg acacatcaaa aatccgcccg aaaaatatca taaccggcaa
      181 aagccaggga taaacatcca gaaagtcaaa catattcaat ttataatttt atgattttat
      241 tgtttcgaat ttataatctt ccaaaatata ttgttattta acgatagtcc tcaaatagga
      301 aacaaagcct gcaagatctt cctccgtagt atcccacgaa cagacaaggc gtctttcgtt
      361 ctgctccaca tcccagtcgt aaaagaaata gcgctcacga agctgagttg tttcttcttc
      421 cgaaagagta aaaaatacgg cattcgattg aacttcctgc gtcagcttaa ctccggcctt
      481 tcgcatcccg gctgccagct tttgtgccgc agcattcgaa ttttgtgcgt tttccagcca
      541 gataccctcg ttgaagtagg gaataaactg tgccgatata aacctcattt tggaatacaa
      601 ctgggaagcc tgcttgcggt aataccttat gttttctgcc agttctttgc gtaagggaac
      661 aagtacttcg cctaccatta gtccgttttt cgtccctcca aaagtaaaga tatccactcc
      721 gcaatccacc gtcatctctt tcactgaaac attgagaaaa gcacaggcat tggccatacg
      781 agtcccatca acaaaaaggt acataccgtg ttcgtgtgcg agatcggcaa gcagctttat
      841 ttcccggggc gtataggctg ttcccatctc ggttgtttgc gagatagcga ttaccttcgg
      901 ctgggaatga tgttccacac caaatccgtg caataacgga cgaaccatct cgggagtaag
      961 ttttccgtcg gacgttacaa tttccttcaa cgcacaaccg gtgaatttgg tgggagcacc
     1021 acactcgtcc actgcaatat gggcggttga ggcgcaaatg atgtgatgaa agggcaacgt
     1081 acaggcttgc aaagcagtaa tgtttgctcc ggtaccgtta aaaacaaaaa ggggttcaat
     1141 atcctctttt ccaatcagtc gtttcactgc tttctccgct tcccgtgtcc agtcatcatc
     1201 accgtaacca attgtatgat ttctgttggc atcaattatc gcctgcaaca tcagcggatg
     1261 gacacccgaa ttattatcgc ttccgaaact tcgcataaat ttttattgta aatgaaacac
     1321 aaaggtacaa aacattgcaa agttaacccc tatttatttg tcccattatt ggctttcatc
     1381 tactttttca ccggtaccgg catcttttcc gtatccgttt ttgcagaact catccgtaag
     1441 ggattggata catcctcaaa aatttccaga ccgaaatagg agacagccgc cgttacgtga
     1501 tcgaaaatat ccgacatgat gttctcatat tccgtccagc tggtagtcgc cgtaaaaaga
     1561 taaagttcca gcggtagtcc ctgggcagta ggagccagct gacgtaccaa aagcaagagt
     1621 tcccgatgta cctggggatg gtttttcaga tacaactcca cgtattttct gaaaaggccc
     1681 atgttggtaa aattccgacc gttcagggga aggcttctgt cagcacccgt tgcgttgttg
     1741 aacgaatcga taatttgttt tcgctcatcg atatagcctg aaagaagttg tatttttctg
     1801 aaatgttcca actcctcctc gtttacataa tggatggtgg attgttttat gtttatggct
     1861 ctcttgatac gccgcccacc cgcttcagtc atcccacgcc agttttgaaa agcctccgta
     1921 accagggagt atggaggaat ggtggtgatg gttttatcga aattcaggat cttaacggtc
     1981 gtaaggttta ttttgatgac atcaccgtcc acgccgtact tgggcatggt tatccagtca
     2041 ccgatgcgga ccatgtcgtt ggaagacacc tggatactcg ccacaaggcc cagaatggta
     2101 tccttaaaaa tcaacagtaa tatggctgat gccgcaccca ggccagccag gatagtggtt
     2161 ggggtttttc cgataaaaag agatataatg acgatggcgc caatggcgta gacaaatatc
     2221 cggatgagtt gtccgaaact ttccaccggt ttatccttaa agttgtcttt tgttttaagg
     2281 gtgtctgtga acgcattaat aaccgacacg ctcaaccaca tgaaataaaa gacaaaaaac
     2341 acgtcgaaca ctttgagcga aagcctcatc gcaacaggaa aatcgttaaa gataattgga
     2401 atagctcctt tgattatgct gatggggatg atcatcgcca agtacttcgg gaacttgttg
     2461 tttatcaagt ggcggctgat ggtcagacgg tttattttta cggctttgtt gagaataacc
     2521 gtaataagct tgcgcaacat cgtttgcatc acatacacga gtacaacgag aatgacagac
     2581 aggaagatag agttgataac cccaatgtag gtgtccggga cgcctgtacc gtgaagaaaa
     2641 tccttcgacc aaccgctgat caggttcgtt acttgctgcg aaaaactctc taccctgtca
     2701 ctggctacgt tgtctttcaa ttgttccatt tcgtcttgta tcataacgct cttttagtct
     2761 ttccgtttct gaaatacaaa ggtaaacatt attggtatag acaacccttt aaaactctaa
     2821 atgaagctta agaaactgtt ttgtattttc atctcttcaa aataacccgc tactgtgtgc
     2881 acactgaaaa tccaaatcca tcccaaaata ggcctgaaac agggtgtgaa aaattcaaaa
     2941 cagtttctta aatacctatg aaaaacaggt tgtatttaat gaaataaacg tatatttgtg
     3001 tcctcagaaa aggagaacag gcgtcgaata acagcgggat gcaacataac atccttaggt
     3061 tgaccaacaa cctcaaatct gatgtaatta atctaaaaaa aggaaaaatg ggaaaagtat
     3121 tgattatcgg tgcaggcggc gtaggtactg ttgttgccaa taaagtagct caaaacagcc
     3181 ggattttcac ggatattatg ctggcaagcc gcacaaaaag caagtgtgat gccattgctg
     3241 aagatgttaa acggagaacg ggtgttacca tcaaaacggc taaagtagat gccgatagcg
     3301 tacccgaact ggtaacgctc ttcaacgaat tcaaaccgga gctggtcatt aatgtagcgc
     3361 tgccctacca ggatctaacc atcatggatg cctgtttagc atgtggagta aactatttag
     3421 acacagctaa ctacgaaccc aaggatgtag ccaaattcga atataaatgg cagtgggcct
     3481 atcgggacaa gttccgtgag gcagggctga cagccatcct gggttgcgga tttgatccgg
     3541 gcgtaaccag cattttcacg gcgtatgccg cgaagcatca tttcgatgag atacattacc
     3601 tggatattgt agactgtaat gcgggcgacc acggcaaagc ctttgctacc aacttcaacc
     3661 ccgagatcaa catacgcgaa gttacccaga aagggaaata ctgggaaaac gggaaatggg
     3721 tagagaccga accgcacgag atccatcaac cgctcaacta tcccaacata ggcccaaaag
     3781 agtcgtacgt catttatcac gaagagctgg aatcgcttgt gaaaaatttt ccaaccctca
     3841 agcgcgcacg tttctggatg acctttggac aagaatacct cacgcactta cgcgtaattc
     3901 aaaatatcgg aatggcacgc atcgacgagg tggaatataa cgggcagaag attgtaccca
     3961 tccagttcct caaagccgtt ctgcccgatc cgggcgaact gggagaaaac tacacgggcg
     4021 aaacatctat cgggtgccgt atccgcggaa ttaaagacgg aaaagagcgg acatactata
     4081 tctacaacaa ttgcagccat caggccgctt acgaggaaac aggagcacag ggtgtaagtt
     4141 ataccacggg agttccggca accataggcg cgatgatgtt tatgcagggg ctatggaaaa
     4201 aaccgggcgt gtttaacgtg gaagaattta atcccgaccc gttcatggaa cagctcaaca
     4261 agcaaggcct cccctggcac gaattgtttg atgtggatct ggaagtttga tgtaaatcct
     4321 cacgtcacat gaaagccaaa agtttagtaa atatcagcga ctacggcaaa gaagatatcc
     4381 tccggatact gaaagcagct acagcgttca aagccaatcc caaccgcgat ttattgcagg
     4441 gaaaggtatg cgctacattg ttttttgagc cttccacgcg tacacgctta agtttcgaga
     4501 cggccgtgaa ccggctcagg ggaagaattg tcggattttc cgatgcagcc accagcagct
     4561 ccaccaaagg ggaatcactg aaagatacca tcatgatggt gggcaactac gccgacctca
     4621 ttatcatgcg ccaccacctg gaaggatcgg cgcgctatgc atctgaagtc tctccggtac
     4681 cgatcattaa cgccggtgat ggttccaacc agcatcccac gcaaactttg ctcgatttat
     4741 tcaccatcta cgagactcaa gggacgatgg agaacctcac cattaccatt gtgggagatc
     4801 tgaaatacgg ccgtgccgtg cactcactca ttcagggatt atcccatttc catccatcgt
     4861 tcaactttgt ggcgccggag gaattaaaaa ttcccgagaa gtacaaagtg ttttgtgaca
     4921 acctgaatat cccctacagg gaatataccg aattttcatc cgaagccatt gcttccgcag
     4981 atattcttta catgacccgg gttcagcgcg aacgttttac cgacctgatg gaatacgaaa
     5041 aggtaaagaa catctatgtt cttcacaatg aaatgttgaa caactccagg gataacctga
     5101 aggtactcca tccgcttccg cgtgtgaaag aaatcaatca ggatgtggat gagaatccaa
     5161 aagcctatta ttttcaacag gctgaaaacg gcgtttatac ccgtcaggct gtcatctgtg
     5221 atttgctgga tatccgtctg gattgaataa ggtagattat cataacaaac gtacaagatg
     5281 ggaaaagaat tacaagtagc ggcactggag aacggaacgg caatcgacca tattccgacc
     5341 cgggagttat tcaaggtggt tgccttgctc cagttacaaa agctggataa ccggatcact
     5401 atcgggaata acttaaaaag tagcaagatg ggtgcaaagg gaatcatcaa ggtctccaac
     5461 aaattcttca aggaagacga gataaaccgc attgcactgg ttgcccccaa cgtcaacctg
     5521 aacattatcc gggattacga agtgattgaa aagaaaaaag tgacactccc cgatgaaatt
     5581 atcgaaattg taaaatgcaa taacccgaaa tgcatcacca ataacgaacc gatgaaaacc
     5641 cgcttcgaag tggtggacaa agaaaatgtg gtgttgcagt gccactattg cgaattgaaa
     5701 atcaagaaag aagaaatcgt cttaaagtag ttgccgggta acacgaatac aaatgaaaca
     5761 gaactggaag ccgggaacac tgatctatcc ccttcccgcg gcaatgatat cgtgcggaag
     5821 ccatcccgac gagtacaaca taatcaccgt cagttggctg ggaacccttt gcaccaatcc
     5881 gccgatgtgt tatatttcca ttcgtcccga acggcattcg tacgaaatca tcaagcgaaa
     5941 catggagttt gttatcaacc tcaccaccga agaactggct caggccaccg actggtgtgg
     6001 ggtacgctcg gggaaggatt acaataagtt ccacgaaacg ggattaacac ctgcaaaagc
     6061 agtagcggta aacgctcccg tcatcgaaga atccccgctc tgcatcgagt gccgcgtaaa
     6121 ggaaattatc acgctcggat cgcacgacat gtttatcgcc gatgtggtaa acgtgcaggc
     6181 ggatgacaag tattttgatg ccgaaaccgg aaaattcgat atgcaaaacg ccaggctcct
     6241 cgcctactcg cacggcaatt attacggact gggtgaacat attggaaaat tcggatggag
     6301 tgtgaagaaa aaaaaatgat aagagttaaa taaaccatat gcccgtaaat ttacccgata
     6361 aactcccagc catagagatc ctgagcaagg aacatatttt cgtgatgagc gacctgcgtg
     6421 cctccacgca ggatatccgt ccgttgaaga tactcatcct caacctgatg ccgcttaaga
     6481 tcactaccga aaccgattta ataaggctgc tatccaactc tcccctgcaa ctggaaattg
     6541 agtttctggg attgaaatcc catacgccta aaaatacacc catcgagcat ttgcagacgt
     6601 tttacaaaac gtttcctaaa gtaaaagaaa gctattacga cggctttatc gttaccggcg
     6661 cacccgtgga attattgccg ttcgaagaag tgcggtattg gcaggagctg accgggatct
     6721 tcgattgggc ccgtacgcat gtgacctcca cgtattacat ctgctgggca ggacaagcgg
     6781 cgatgtatca tttttatggc atccggaaat atccgctgga caagaaaatt tttggagtgt
     6841 tcccacatac cgtcaacgat aaagcgttcc cgcttttccg cggattcgac gacgagtttt
     6901 ttgctccaca cagccgccac acgacggttt ctgccgagga gatttcccaa caccccgaac
     6961 tgactatcct ctcacagtct gaagaagcag gagtatacat cgtggcctcc cgcggtggac
     7021 gtgagctcta cgtaaccggg cattctgaat attcacccct cacgctacac aacgaatata
     7081 cgcgtgatat gcaaaaggga ttggactcgg tggaactccc caaaaactat tacaaggata
     7141 acgacccaca aaaacaaccg gttgtcttgt ggagaagcca cgcaaacctt ttgtttataa
     7201 actggctcaa ttactttgta tatcaggcaa ctccatttga tttgaaaaag ataaaagagt
     7261 taggggattt aacctgatcc cgcttggatg aatggaaaga tgaaagctcc acgaatcgtt
     7321 gaacttctcg cccctgccaa aaataaggag atcggaaaag aagccatcct tcacggtgcc
     7381 gatgccgtct acataggtat ctcgggattc agtgcacgga tggctgcagg aaacagtatc
     7441 gaagatattg ccgagttggt ggaatttgcg catcagtaca acgccaaagt gtatgttgcg
     7501 ttgaacacca tcctttacga ccatgaactc ttacaggtgg agaagctcat ccgggagtta
     7561 taccgtatac acgccgatgc cgttatcgtc caggacatgg gcattttgca actgaacctg
     7621 cctcctatcc cgctccacgc cagcacgcag accgataatc gtaccgtgga aaaagtgcag
     7681 tttttagaaa acgcgggatt cacacaggta gtgcttgcac gggaattatc ccgggatcag
     7741 attgccgaaa tttcgtcaca aaccagcatt gcgctggagg tttttgttca cggtgccctc
     7801 tgtgtttcct acagcgggca atgctacatc agccaagcca taacgggtcg gagcgccaac
     7861 cgcggagagt gtgcacaaat ttgcaggcta ccttttgatt tgcaggatgc cgatgggcgt
     7921 attgttcgaa aaaatgcaca cctgctttcc ttaaaagact tcaatcagta cgataacctc
     7981 gaagagctgc tggatgccgg cgtttcatcg ctcaagatag aaggacggct gaaagacgtt
     8041 acgtatgtta aaaacgtagt tgctgcctac cggcagcgat tggacagcat tttcaggaaa
     8101 cg
//
LOCUS       DAOSDR010000093         8084 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrimonas sp. isolate AMR_MDS_1826
            NODE_3300_length_8084_cov_4.547640, whole genome shotgun sequence.
ACCESSION   DAOSDR010000093 DAOSDR010000000
VERSION     DAOSDR010000093.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863355
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrimonas sp. (wastewater metagenome)
  ORGANISM  Petrimonas sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Dysgonomonadaceae; Petrimonas.
REFERENCE   1  (bases 1 to 8084)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8084)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:10:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,720
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 35
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8084
                     /organism="Petrimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3300_length_8084_cov_4.547640"
                     /isolate="AMR_MDS_1826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2023866"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..679
                     /locus_tag="PLI30_05135"
     CDS             <1..679
                     /locus_tag="PLI30_05135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019539435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HOI78974.1"
                     /translation="NWFWLGLSGVVGFFLGDLFLFKSYTLIGSRTSQLIMSLAPMITA
                     IIGWLFLSEILPVKSILGIIVSISGIMIAVAGKKLKLNIPLKGFLYALGGALGQAVGL
                     VLSKKGMGDYDAIAATQIRAIFGFVCFFLLVTFLKRWRRVSLAVKDRTSMKSITLGAV
                     FGPFVGVTLSLYAVQHTHTGIAATLMALVPIFIIVPSAIMFNEKITARQVIGAVISIA
                     GASIFFL"
     gene            complement(686..1837)
                     /locus_tag="PLI30_05140"
     CDS             complement(686..1837)
                     /locus_tag="PLI30_05140"
                     /inference="COORDINATES: protein motif:HMM:NF025420.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4249 family protein"
                     /protein_id="HOI78975.1"
                     /translation="MKRKYISFLITTILSLSCERMLEVNFSDEKPLIVMNGVVEPDKP
                     ISVAVSKSFLFTDTDSTAPYLKDVSVELHVNDTFVEKLQRFTIDTAKYRARRGNSYFR
                     SESHVKVGDRVKIKASAPGLETAWVETVIPTPAAIEKVNTATFFVPATGNNTFGFYWN
                     PYGEYSWNSDSGWGYGGYYIPVPEIFYEPFYRRMRLHIGVRKQKNDVAHYFLLRIIAE
                     NTEYKDYPFNLPVETQDDPIFARDPKNLFLEKLFNNNYSTNSSSVFTDNLFTENAYTL
                     NVSTGELYTVKVEHTKPEKEGEKPEYLSHEVKNSPIEVWIYTLSHDYYSYLKSVEKWN
                     YDEEFDVISEPRVTYSNVHNGIGFVGSMSYTSKRIQIPPYPGGKNTVPR"
     gene            complement(1925..4660)
                     /locus_tag="PLI30_05145"
     CDS             complement(1925..4660)
                     /locus_tag="PLI30_05145"
                     /inference="COORDINATES: protein motif:HMM:NF025092.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOI78976.1"
                     /translation="MKFSKRYTILVLLAVCSFLGASAQQNITINVEKQPVASVLQKIE
                     KQSGYTFSYNPALLRNFPPVTLQVENMLLQPLLEKLFGKTDIQFIIRDKYIILKERQK
                     NVTISGFIHESGSRETLIGANVFDLLSQKGAVSNNFGFFSLSLPPGEVRLRGSYVGYT
                     SQEITFVVDNDTVIHFFLEPTFLLKEVVIEGRQTGPLHNVETGKITLTTQTLQALPSF
                     LGEKDVIKTLQLLPGVAAGTEGVAGMYVRGGNLDENLYLVDGNPLYHVNHLGGMFSTF
                     NPEAVKTMDFYKGSFPARYGGRLSSVVDVRMNDGDMNKLGGTASIGLISSRFNFQGPL
                     VKEKTSFNVSLRRTYLDLIVRPALYFVGKKDEKDNPDDYNRSDVGYFFYDFNVKVNHK
                     FSEQSRLYLSVYDGKDKLFLNMNAKSEGSYNLDSDNNSPNPAFNTHHEINKNKTNFDT
                     GWGTRMASLNWAYAINNKLFSNVALIYSRYSSDIVMKSSSEKELTLSEENSDKIERRV
                     DKTKENTHYRSGIQDIGYRLEFDYTPVNNHFIRFGTSLLHHRFRPEQSGITLITENDT
                     EKRNDTVTFANERVLVKELSFYAEDEMILNERMKVNAGVHFSGFLVQGRSYISAQPRL
                     SARYLLNNDWSVKVSYGKMNQYVHLLQSSKISLPNDLWVPITKNVKPMVSHQVSGGLF
                     GRWRRFDLSLEGYYKSSRNQVEYREGTSLLSGNLNWEQRIAQGLSKSYGVEMMASKTF
                     GNTSGWLGYTLSWSNRHFPNGEINRGLVYPAKYDNRHKINAVVMRRFGRKFDVSTSWI
                     YSTGNWATLAKQKYINDMGEETDYIDKLNNFKMPSYHRLDLSFNYYRHKKKGRLGIWN
                     LSIYNAYSHHNSFILVPATEAEFFTREEKQDRSKPVLKSLSIFPIIPSFSYTYKF"
     gene            complement(4679..5509)
                     /locus_tag="PLI30_05150"
     CDS             complement(4679..5509)
                     /locus_tag="PLI30_05150"
                     /inference="COORDINATES: protein motif:HMM:NF016648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR family protein"
                     /protein_id="HOI78977.1"
                     /translation="MENQSRHNIDEGIRFVARYYKPNVFDSRKGWGKIQKYIAPERNI
                     LAMRMLYGTAAAVALLLVVSVFYFTSNTGTRLVAKADNASYVLPDSSRIGMQQGAKLK
                     YGKNFGKAERRVSMSGEISFAVARNEALPFVVSTPGAEIRVLGTEFTVLADDAETRLG
                     VVSGTVQFTPESPVIPLLCTAGMTVHYTSVTETVKVVSPGSSMEINGKDRSLVFNNTQ
                     LKDVALVLSHFYNVQVELPEEESTLTFTSSFAQKNSIEIVDIINLTLDTHLIIKKGSN
                     "
     gene            complement(5529..6029)
                     /locus_tag="PLI30_05155"
     CDS             complement(5529..6029)
                     /locus_tag="PLI30_05155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI78978.1"
                     /translation="MCRKIIVSLCLIICPVFVVNTQARDSDTDLRIGLGFSTLKQGNV
                     FLVLSEYELDAKLSDYFTVAPSIVLSVADKWKSDSEKLFQANMNVFLSPFRNNRRNDF
                     RIGGGLSFYKFNQPVIRSAFGFNLIVEDSYSINDRFFIGVKAFMQPYFNRESSSGVLL
                     KAGINL"
     gene            complement(6105..6614)
                     /locus_tag="PLI30_05160"
     CDS             complement(6105..6614)
                     /locus_tag="PLI30_05160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018336469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma-70 factor"
                     /protein_id="HOI78979.1"
                     /translation="MLISKKQFSEYYHSYYRQLCLHALHFMCDTDEAEDVVQETFVNL
                     WDKREQLETIKSVKAYLYTAVRNNCLTRIRDAKPTTSLDKLSGEEFLTEDEQMERAEM
                     EARIWKMIDELPERRREIFLMAKRDGMRYKDIAEQTGLTVKTVENHVLRAMQSLRAKD
                     IKAYLFFFA"
     gene            complement(6804..7223)
                     /locus_tag="PLI30_05165"
     CDS             complement(6804..7223)
                     /locus_tag="PLI30_05165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019538383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HOI78980.1"
                     /translation="MTEFISDVKNISHNDVDIFRVLSDLSKLDLIKDQIPGDKIRDFR
                     FDSDSCSFRVDPIGEVRFCVIEREPCKLVKFKSENLPFDVFLWIQLVQKAEKDTKMKI
                     TLRADLNPFIKGMVSKPLKEGLEKISDVIASLPYDRI"
     gene            complement(7220..7873)
                     /gene="pyrE"
                     /locus_tag="PLI30_05170"
     CDS             complement(7220..7873)
                     /gene="pyrE"
                     /locus_tag="PLI30_05170"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019538382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HOI78981.1"
                     /translation="MNTVEKLTAEKLLKIKAVKLQPSNPFTWASGWKSPIYCDNRKLM
                     SYPATRNFIKVEFARIILEKYPQVEAIAGVATGAIAPGAIVADALGLPFVYVRSAPKD
                     HGLENLIEGDLKPRQKVVVIEDLISTGQSSLKAVEAIRQSGSDVLGMVAIFTYGFPAA
                     ETNMKEARVELTTLTHYDAILDEALLIDYIAESEVKTLQDWRRNPETWGTTTKKMNK"
     gene            complement(7962..>8084)
                     /locus_tag="PLI30_05175"
     CDS             complement(7962..>8084)
                     /locus_tag="PLI30_05175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019538381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HOI78982.1"
                     /translation="KHILLIDDIITTGSTLEACGYALLKSPDIKISVATLGEVF"
BASE COUNT         2215 a         1868 c         1633 g         2368 t
ORIGIN      
        1 caactggttc tggctagggt tatcgggcgt ggtgggcttc tttctgggcg acctgttcct
       61 ctttaagtcc tacaccctga tcggttcgcg cacctcgcag cttatcatga gcctggcgcc
      121 gatgataacg gcaattatcg gctggctttt tctgtcggaa atactgccag taaaaagtat
      181 cctggggatt attgtcagca tttcgggaat tatgattgcc gtcgctggca aaaaactgaa
      241 actcaatatt ccgctcaagg gtttcctcta tgcgcttggc ggcgctttag ggcaagccgt
      301 cgggttggtg ttgagtaaaa aagggatggg cgattacgat gccattgcgg caacgcagat
      361 aagggctatt ttcggatttg tttgcttctt tttgctggtc acttttctga aacggtggcg
      421 aagggtctcc ctggccgtga aagaccggac aagcatgaag tccatcaccc tgggagctgt
      481 tttcgggccg ttcgtgggcg taacgctatc gctatatgcc gtgcagcata cccataccgg
      541 cattgccgct acgttgatgg cactggttcc catttttatc attgtgcctt cggccatcat
      601 gttcaacgaa aaaatcaccg cccgccaagt aatcggggca gtaatcagta ttgccggggc
      661 aagcatcttc tttctgtgag aaagcttacc ttggaacagt gtttttaccg cctggataag
      721 gtggtatctg aatgcgctta gatgtatatg acatagatcc cacaaacccg attccgttgt
      781 gtacgttgga gtaggtaacc ctgggttcgg agataacatc aaactcttcg tcgtaattcc
      841 acttttcaac cgatttcagg tacgaataat aatcgtgaga cagcgtgtaa atccacacct
      901 cgattggcga atttttcact tcgtgactta aatattccgg tttctccccc tccttttccg
      961 gtttggtatg ttccaccttc accgtgtata gttctccggt ggagacattt aacgtataag
     1021 cattctctgt aaacaagtta tctgtaaaaa cagatgaact atttgtggag taattgttat
     1081 tgaacagctt ttctagaaaa aggtttttag gatctctcgc aaaaatggga tcatcctgtg
     1141 tttcgacagg caagttaaaa ggataatctt tatactccgt attttccgca atgatcctca
     1201 aaagaaaata atgtgccaca tcgttttttt gttttcgaac cccaatatgc aagcgcatcc
     1261 tccgataaaa cggctcgtaa aatatttccg gaactggaat ataatatccg ccgtaccccc
     1321 atccgctgtc tgaattccag gaatactcgc cgtatggatt ccagtagaat ccgaacgtat
     1381 tgttgcctgt tgccggtacg aaaaacgttg ccgtatttac tttttcaatc gcagccggtg
     1441 tgggaataac ggtctccacc catgcggtct cgaggcctgg tgccgaagcc ttaatcttaa
     1501 cccggtcgcc gactttaacg tgtgactcgg aacggaaata agaatttcct cttcgagccc
     1561 ggtacttggc agtatctatg gtgaagcgtt gcagtttttc aacaaatgta tcgttaacgt
     1621 gcaattccac cgaaacatct ttcaaataag gcgcagtgct gtcggtatcg gtgaacaaga
     1681 aacttttgct gacagcaacg cttattggct tatcgggttc aacaactcca ttcatcacaa
     1741 taagcggctt ttcatcggaa aaattcactt caagcatacg ctcacacgac agcgatagaa
     1801 tcgttgttat cagaaacgaa atatattttc tcttcatttt agaatctgtc ttgaatttta
     1861 tatttctatt tatttctact ttcttcacgt gtcgagagac ctatgaacca cccgatacag
     1921 tcggtcaaaa cttatacgtg taagaaaatg atgggataat agggaaaatc gacaaacttt
     1981 ttaataccgg tttactcctg tcctgtttct cttcccgagt aaaaaactcg gcctcggtag
     2041 cgggcaccag aataaacgaa ttgtgatgcg aatacgcatt gtaaatactt aaattccaaa
     2101 ttcccaaccg ccctttcttt ttgtggcggt aataattgaa acttaaatcg aggcggtgat
     2161 aggaaggcat tttaaagttg ttgagtttat cgatataatc tgtttcctct cccatgtcgt
     2221 taatatactt ttgctttgcc agcgtagccc agttaccggt agaatatatc caagacgtgc
     2281 tcacatcgaa tttcctcccg aatctgcgca tcactaccgc atttattttg tggcggttat
     2341 cgtatttcgc cggatagaca agtcctcggt ttatttcacc gttcgggaaa tgacggttcg
     2401 accacgatag cgtatagccc agccaccccg aagtatttcc gaaagtcttg ctcgccatca
     2461 tttccactcc gtatgattta cttaatcctt gagctatacg ctgttcccaa ttaagattac
     2521 ccgaaagcaa ggacgtacct tccctatatt ccacttgatt cctggaagat ttgtaatatc
     2581 cttccaatga aaggtcaaac ctgcgccaac gcccgaaaag tccacctgaa acctgatgtg
     2641 agaccatcgg ttttacattt tttgtgatgg ggacccacaa atcgttaggc aaacttattt
     2701 tactgctttg caacaaatgt acatactggt tcattttgcc atacgatact ttcaccgacc
     2761 aatcgttatt gagcaggtaa cgtgccgaaa gacgtggctg tgcgctgata taagatcttc
     2821 cctgcaccag gaatccagaa aaatgtacgc ccgcattcac tttcatgcgt tcgttcaaaa
     2881 tcatttcatc ttcagcatag aacgaaagtt cctttaccaa aactcgttcg ttggcaaaag
     2941 taacggtatc gtttcgcttc tctgtatcgt tttctgttat caaggtaatt ccgctttgtt
     3001 cggggcgaaa ccggtggtgc aacaacgatg tcccgaaacg aataaaatga ttgttcaccg
     3061 gtgtatagtc aaactccagg cgataaccta tgtcctgaat tccggaacgg taatgagtat
     3121 tctcttttgt tttatcgacc cgtctctcaa tcttgtccga attctcttcc gatagagtta
     3181 attctttttc gctactcgat ttcattacaa tatccgacga gtatcggctg taaattaatg
     3241 caacattgct aaacaactta ttgttaatag cataggccca attgagcgat gccattcgcg
     3301 tgccccaacc ggtgtcaaaa ttggttttat tcttatttat ttcgtgatgt gtgttgaatg
     3361 ccggattagg agaattgtta tccgaatcta aattgtaaga accttcactt ttggcgttca
     3421 tatttagaaa gagcttgtcc tttccgtcgt aaacacttaa atacaatcga ctttgttctg
     3481 aaaacttatg attgactttg acattgaaat cataaaaaaa atatcccaca tccgatctgt
     3541 tataatcatc gggattatct ttttcatctt tcttccctac aaaatataat gcagggcgca
     3601 cgattaaatc taaatacgta cggcgaagag aaacattgaa tgaagtcttc tctttcacta
     3661 acggaccttg aaaattaaat cgcgatgaaa tcaacccgat agaggccgtg ccgccaagtt
     3721 tattcatatc gccgtcattc atacgcacat ctactaccga cgaaagacga ccgccataac
     3781 gcgcaggaaa gctgccttta taaaaatcca tggttttcac cgcctcagga ttgaatgtgg
     3841 aaaacatgcc acccaggtga ttcacgtgat aaagcggatt cccatccacc aagtagagat
     3901 tttcatctaa atttccaccc cgcacgtaca tacctgccac accttcggtc ccggcggcca
     3961 cccccggcaa aagttgcagg gttttaatca catctttttc tcccaaaaaa gaaggcaaag
     4021 cctgcagcgt ctgcgtagta agtgttatct ttccggtttc gacgttatgt agtggtccgg
     4081 tttgtctacc ctctatcacc acttctttaa gcaaaaaagt aggctctaaa aaaaaatgga
     4141 taaccgtgtc gttatccaca acaaaagtta tctcctgcga tgtgtatcct acgtaacttc
     4201 cccttaaacg gacttctcca gggggaaggg aaaggctgaa aaaaccgaaa ttgttgctta
     4261 cggctccttt ttgcgaaagc aagtcaaata cattggcgcc gataagtgtt tcgcgcgatc
     4321 cggattcgtg gataaatccg cttatcgtaa cgttcttctg acgctctttc aaaataatat
     4381 acttgtcgcg gataataaat tggatatcgg tcttgccaaa aagcttttcc agtaaaggct
     4441 gcaacaacat attctctacc tgaagcgtta cgggcggaaa atttctcaac agtgccggat
     4501 tgtatgaaaa cgtatatcct gactgcttct caatcttttg cagaacggaa gcaaccggct
     4561 gtttttcaac gttaatcgta atgttttgct gggctgatgc tcctaaaaag gagcaaacag
     4621 ccaacaaaac aaggatagta tagcgtttcg agaatttcaa tgtttttgct ctttattctt
     4681 aattcgaacc cttctttatt atcaagtgcg tatccagtgt taaattaata atatcaacaa
     4741 tttcaatact gtttttttgt gcaaatgacg aggtaaaagt gagcgtggat tcctcttccg
     4801 gcaattctac ctgcacgtta tagaagtgcg acaaaaccaa cgccacgtct ttcagctgcg
     4861 tattattgaa gaccagggag cggtcttttc cgttgatttc cattgagctt ccgggcgaaa
     4921 caacctttac ggtttcggtt acggaagtgt aatgcacggt cataccggcg gtgcacaata
     4981 gcggaataac gggactttcg ggcgtaaact gcactgtccc cgacaccacg ccaaggcggg
     5041 tttccgcgtc gtcggccagt acggtaaatt ccgtgccgag cacccggatc tctgctccgg
     5101 gtgtggaaac gacaaagggc agggcttcgt ttcgggcaac ggcaaacgaa atttctcccg
     5161 acatggaaac acggcgctct gccttaccga aatttttacc gtatttcagt tttgctccct
     5221 gttgcattcc gatgcgggaa ctgtcgggca acacatagga agcattatcg gctttggcta
     5281 ctagtcttgt gccggtattt gacgtgaaat aaaaaacact tacaaccagc aataaagcga
     5341 cagcggccgc agtgccataa agcatcctca tagcgagaat gttgcgttcg ggtgcaatgt
     5401 atttttgtat ctttccccat cctttccggg aatcaaaaac attgggcttg taataccggg
     5461 ctacaaaccg gataccctca tctatattat gtcttgactg gttttccatt attttcagtt
     5521 ttggcaatct acagatttat tccggccttc aacaatacgc cggaactgga ttctctgtta
     5581 aaatacggct gcataaatgc tttgactccg atgaaaaatc gatcatttat tgaataagaa
     5641 tcttcaacaa ttaaattaaa accgaatgca ctgcgaatta caggctggtt aaacttgtaa
     5701 aaacttaatc cacccccaat cctgaaatcg tttctcctgt tattgcgaaa aggagataaa
     5761 aaaacattca tgtttgcttg aaataatttt tccgaatccg acttccactt atccgcaaca
     5821 cttagcacaa tggatggagc aactgtaaaa taatctgaca gtttagcatc aagttcatac
     5881 tcactaagca ccaaaaaaac atttccctgc ttaagtgtag aaaatcccag tcctattcgc
     5941 aggtctgtgt cactatcccg ggcttgtgta tttacaacga atacaggaca aataataagg
     6001 caaagggaca cgataatttt tctacacatt tcttctcttc cattactttt tctatttata
     6061 agatgcaaaa aagtataaaa tccctctttc taaagggaaa tattttaagc aaagaagaaa
     6121 agataagcct tgatgtcttt tgcacgtaac gactgcatgg cacgcaggac atggttctcc
     6181 acagttttta cggttagccc ggtttgttcg gcaatgtctt tgtagcgcat cccatcgcgc
     6241 ttagccatta aaaagatttc gcggcggcgt tccggaagtt cgtcgatcat cttccagata
     6301 cgtgcttcca tctcggcacg ttccatttgc tcgtcctccg taagaaactc ttctccggac
     6361 aatttatcga gagaggtagt tggcttggcg tcacgtattc gggtaaggca gttgtttcga
     6421 acagcagtat aaagatacgc tttcaccgat tttatcgttt ccagctgttc ccttttatcc
     6481 cataagttca cgaatgtttc ttgcacaaca tcttccgcct catcggtgtc gcacatgaaa
     6541 tgcaaggcgt gcaagcaaag ttgacgataa taactgtggt aatattccga aaactgtttt
     6601 tttgaaatca gcatcaaatc tttagtaata gaataagttg caaaactagt aaaaaagggg
     6661 gaatattaaa aagaaaatcc ggttggaccg tacgcacgct taaagtgaac gaacggccgg
     6721 atcttattca tcgtaggttt aaacaacact atcgcagcag gtaaaataga atacggttta
     6781 aaacgttcca caaaaacgac acatcaaatc ctatcgtacg gtagcgatgc tataacgtcg
     6841 gaaattttct ccagtccttc tttcagtggt tttgagacca ttcccttgat gaatggattg
     6901 aggtcggcgc gaagcgtaat tttcattttg gtatccttct cggctttctg cacaagttgg
     6961 atccacagga aaacatcaaa cggcaagttt tcggatttaa acttgaccag tttacagggt
     7021 tcccgttcta tcacgcaaaa ccttacctct ccaatcgggt cgacgcgaaa cgaacaggaa
     7081 tcactgtcga acctaaaatc cctgatctta tccccaggta tttggtcctt tatcaaatcg
     7141 agcttgctta aatccgagag cacacgaaag atatccacgt cattgtgaga tatatttttc
     7201 acatcgctga taaattcagt catttattca tttttttggt ggtggttccc cacgtttcgg
     7261 gatttctacg ccaatcctgc aatgtcttca cttcagattc agcgatataa tcaatcaaga
     7321 gcgcttcgtc cagaatggca tcgtaatgag tcaaagtggt caactccact cgagcctctt
     7381 tcatgttggt ttctgccgca gggaaaccgt acgtaaatat cgctaccatt ccgagcacat
     7441 ccgatccgct ttggcgaatc gcttctacag ccttcaggct gctttgtccc gtagaaatga
     7501 gatcttcgat aactacaacc ttttgccggg gtttcaaatc accctcgata agattttcca
     7561 acccgtgatc tttcggcgcc gaccgaacgt aaacaaacgg aaggcccaac gcatcggcaa
     7621 caatcgctcc tggagcaatc gctcctgtag ctacccccgc aatagcttca acctgcggat
     7681 acttttcaag gatgatccgt gcaaattcta cttttataaa gttgcgggtg gcaggatagg
     7741 acatcagttt cctgttatca caatagatgg gtgacttcca ccccgatgcc caggtaaacg
     7801 ggttggatgg ttgtaacttg accgctttta tcttgagaag cttttcggct gttaattttt
     7861 ctacagtatt catattttcc aaacttaaaa atgaaatgca aaaatagtgt ttttaaagca
     7921 aaaaatggca caaaacatga gtggtaaaat aaaatatcca ttcaaaacac ttctccaagt
     7981 gtcgcaacac ttatctttat atctggactt ttcagcaaag cgtaaccaca agcttccagc
     8041 gttgagcctg tggtaatgat atcgtcaatc aacaaaatat gttt
//
LOCUS       DAOSDR010000094         8083 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrimonas sp. isolate AMR_MDS_1826
            NODE_3301_length_8083_cov_4.029273, whole genome shotgun sequence.
ACCESSION   DAOSDR010000094 DAOSDR010000000
VERSION     DAOSDR010000094.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863355
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrimonas sp. (wastewater metagenome)
  ORGANISM  Petrimonas sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Dysgonomonadaceae; Petrimonas.
REFERENCE   1  (bases 1 to 8083)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8083)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:10:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,720
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 35
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8083
                     /organism="Petrimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3301_length_8083_cov_4.029273"
                     /isolate="AMR_MDS_1826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2023866"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(137..1096)
                     /locus_tag="PLI30_05180"
                     /pseudo
     CDS             complement(137..1096)
                     /locus_tag="PLI30_05180"
                     /inference="COORDINATES: protein motif:HMM:NF014212.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1285..1791)
                     /locus_tag="PLI30_05185"
     CDS             complement(1285..1791)
                     /locus_tag="PLI30_05185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002559261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4625 domain-containing protein"
                     /protein_id="HOI78983.1"
                     /translation="MKKVFFTTVLAISAFVFLSCEKESIDAERPVIKLIAPEEEEGIK
                     PGSDIHFDAVLTDNVALKSYKINIHGAFDGHSHSAATRAAGDVVAFEKTWLEADFIQL
                     GDEPIAGKKEARLHHHHMVIPAEIDGKPIREGHYHFTIYCTDEAGNESFIAREIEISS
                     SAEEHTYH"
     gene            complement(1804..4179)
                     /locus_tag="PLI30_05190"
     CDS             complement(1804..4179)
                     /locus_tag="PLI30_05190"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOI78984.1"
                     /translation="MKKIVLAMTFALSFLLSFSQTYSLKGYVCDALSKEPLPGAIVVL
                     GPNEIYAVSGRDGAFSVDLPGKTPHTLEVRFTGYELLTTSIILTSDTVLHFHLETTSY
                     SLNEVVVHGKNGHGRLLSAITAKEIDRSFFMKNNSASFSKSLSKVAGVSSMDIGANVS
                     KPVIRGLGFNRIAVADKGVVQQNQQWGADHGLDIDQYDVDKVFIHKGPMSLFYGSDAI
                     GGVIEILPVDVPSENMVWGDVSLIAKSNNNLSGITAMTSVKRGRWFLRGRLTAQRFAD
                     YRVPADTVTYLTWKLPLYGRRMKNTAGQELNAALSANYSQENISSWTHISNVFSKNGF
                     FPGSHGIPDLKRLTPDGNSFNIGYPYSTSNHFKISNGTEIDWDNSSLHFDIGFQQNNR
                     AEWSKFHTHYGNQAPPQVSPDLELSFSLQTMSVNSRLSFGKDSLWTKTAGMSVEVQRN
                     RVGGYSFLMPDFERYTAGAYAATSYKVSPKLMLTGGVRFDSGKLNIRGFYDDTLADFL
                     QLGGYDADDVWYYAQRADALRKNFHSFSGALGAVYDPNRLSSLKINIGRSFRFPTANE
                     LASNGVHHGAFRHEKGNNSLKPETGWQYDLGYSLKNRRLNLSINHFASYFPNYIYLNP
                     TGQWSVLPHAGQIYEYTQSRVFLAGGELEAAYRFTENLLLSANWEYVYNRNIDNGYPL
                     PFSPPVDMLLALIYSNHGKGPLIQYSASLENQTVFAQNRIANNEEKTLGTSLINLLCS
                     MNWKVGDFRFFTDFQVQNIFDTPFYNHLSYYRKLNIPEPGRNIQFILKIPF"
     gene            complement(4199..4600)
                     /locus_tag="PLI30_05195"
     CDS             complement(4199..4600)
                     /locus_tag="PLI30_05195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019541352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HOI78985.1"
                     /translation="MTVENILRDYHLKSTTCRKFILNELLRSQTALTEHEIKTSFPDL
                     FDRVTFYRTLKTLEDAGVIHKIVLQDNTIRYAVSKKPFHEQDIHSHFHCCVCNEVFCI
                     QNKSQINIELPQGFVQHRVSMIVEGVCAACN"
     gene            complement(4698..5528)
                     /locus_tag="PLI30_05200"
     CDS             complement(4698..5528)
                     /locus_tag="PLI30_05200"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOI78986.1"
                     /translation="MKYILNFLLLLFCSFSISAQTDTYNGWIDKSAKFIENNRLDSAA
                     VALQKAMRLDPANENNAVLLLNLGILQRQLRQTDDAYISFTASLGNNPDPVLVLHNRA
                     SLLCDLGRFDEAMEDYNSIIDKQPSDVEAYYRRGLLFLEKNDRESAEADFRTCESTDP
                     GNLFTKLSKALIFKLDDNWTEAEKIYTDIINTTVKPSSAYYLNRAECYVNTDRFSKAA
                     ADLHAVESDEKANPYFYVLRGRLRLDQFDKFAARVDFEKAKELGYDAELADKWIERAR
                     "
     gene            5612..6520
                     /locus_tag="PLI30_05205"
     CDS             5612..6520
                     /locus_tag="PLI30_05205"
                     /inference="COORDINATES: protein motif:HMM:NF013117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel"
                     /protein_id="HOI78987.1"
                     /translation="MLLNTFVSADSAVVKTGGKVAEMIKDGRFEELGQQSLTWGIDFL
                     GKLAVALLIFFVGKWIIGKIRKLADKIMSRRQMDIALKGFLKNLLDIFLFVLLVMLII
                     NVVGTQTVSLAALIASAGLAVGLAVKDNLANFAGGVMLLFNKPFKGGDYIEAQNLAGT
                     VQSIGILYTTLTTADNKTIYIPNGPLSTGNIINYNTQTTRRVEITASIEYGTDVDIVK
                     KMLLDIANRHPLVLKDPEPFARMTKMNEDSIDFSLRAWVNLNDYWNVTHDLNEEVYKE
                     INARGLVIPFRQMTVHLANSTEQNAN"
     gene            6520..>8083
                     /locus_tag="PLI30_05210"
     CDS             6520..>8083
                     /locus_tag="PLI30_05210"
                     /inference="COORDINATES: protein motif:HMM:NF012700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI78988.1"
                     /translation="MPVTERIAKYRELAENQLNAAARYKKELNANSLLRFFVFLTGSA
                     LTYFFHQNTLLAVLFAAVTGVCFLALLARHKNLLFKKAKSEALARIAGNELQAFVYDF
                     SPFDGAPEHVDPAHSFSFDLDIFGERSVFQMLNRTSLAMGKEALAGIVGSPLKDSGEI
                     RIRQLAVKELSEKEDFCLSFRTIGMLSDDKFFDNRSIKQLCRKQELFPRPSLWKIATW
                     VVPALYVLAIALALAGLTSGAAITLLYFTTLLVSVIPMKKVKQIRSLFDNKAKVLESY
                     ADLLKLMETTSFESVQLHSLQQSIKNERQSASAAIKQLAQYSRNLDLAFAVPVFLLLN
                     PLFLWNVVYALKIEQWMQRHAVEIEKWFSTLAQVDALVSMGTFTANNPDYVFPTFEES
                     LPFRARKLGHPLLPRRKCVKNDINVFRKPFFMIVTGANMAGKSTYLRTAGVNYVLAGA
                     GLPVCAESMSYRPGQLLTNLRTADSLVNNESYFFAELKRLKMIIERLESGEHDLFIIL
                     DEILKGTNSHDKQ"
BASE COUNT         2164 a         1913 c         1841 g         2165 t
ORIGIN      
        1 gtaaaatctt ttccgttaaa taaaagtgtc catccttgtt tttcttcttt gttggtcaat
       61 tcgttcactt ttgccgtgcg cgttgctcca caggaaacaa ggaaaagaat tgataacagg
      121 taaatccatg tagttttcat tgcttaaaat tttttaatga ttcgttattc gtgttttttc
      181 tgatctttat ttcaaacgct ttcacgggat ttatctccag caaaagccgc acttcttcac
      241 gggtaaaacc gttgttcaac aaggcaggga caagttcacg gaaaagagcc gtatatcccc
      301 ggacttcgcc accgccgggc ttcgccggat cgtaccagcc ggcatcgtgc gacagaagta
      361 ctttatgcaa cagattttca ttcttcatcc cggttatcat cttgatgtaa tccggaatgt
      421 tgtcctcgtt cagcccgtcc aagcttatcc aggcacccag acgggctgct tccgcacgtt
      481 ttacggaatc cttttccgct tgcgcatgta cccagataaa agcgtcgggc aacacccctt
      541 cttcttgcag aacggaaagt tgctaatatg ccagagacga cggacccgta tgggatgcga
      601 taaccaatcc ggttttaagg tgggttttgg cggcagccga tatcaacttg gtattcattc
      661 ccgataagtt tgtactattg accccgattt tgataaaccc cggtttaacg gttgtgccgt
      721 ctattccgtg ttcccattcg tttatccatc tttcggccaa ctggccggca ctttcatcga
      781 aagcaaaccg ggggacgaac ttgttgtcca cggctccgta aaatccggtg ttggtaagaa
      841 tattaacgcc cgataatttc gacagctcct gcaacagaag gacatcacgc cccaaataat
      901 cgggagtgca atccacaaag gtctggcaac ccgactcctt cgcttccagc aaaaaaggca
      961 acattttttg aacgacctct tctctcttcc acctgtcggc ggatagggag tcagcaccga
     1021 taaaatcaac cagaagatgc tcgtgcgtga gaaatactcc cgctgagctg gatgggatta
     1081 tcccgttcac cgtcatcacc tgcccgctcc ctcctgaaaa attgcaagac gaaaagaaaa
     1141 gcaggacgaa aagaataact gtttctcccg gattaaattt caacaccctt atatgaattt
     1201 gatattcgat gattgattct acaaatataa aacaaaaaaa gtgggaattg atcccacttc
     1261 cttacttttt taaaaaaccg cttattaatg gtacgtatgc tcttcggcac tggaggatat
     1321 ttcaatttcc cgagcgataa aagactcatt tcctgcctca tccgtgcaat agattgtaaa
     1381 atgataatgt ccttcccgga tgggcttccc gtcaatttct gcgggaatca ccatgtggtg
     1441 gtgatgaagc cgggcttctt tttttcccgc aatcggctcg tctccgagtt gaataaaatc
     1501 ggcttccagc caggtttttt caaaagcaac aacatcaccg gccgcacgtg ttgccgcact
     1561 gtggctgtgc ccgtcaaatg ctccgtgtat attgatttta tacgacttta gggcaacatt
     1621 gtccgttaag accgcatcga agtgaatatc ggatccgggc ttgatgcctt cctcttcttc
     1681 aggggcaatt aatttaatta caggcctttc ggcgtcaatc gactcttttt cacacgataa
     1741 gaaaacaaag gcgcttatcg ctaaaactgt tgtaaaaaat acttttttca ttgtttgatt
     1801 aatttaaaag ggtattttca aaataaactg gatgtttcgt cccggctcgg ggatatttaa
     1861 cttccggtaa taactcaaat gattataaaa aggggtgtcg aaaatattct gcacctgaaa
     1921 atcggtaaaa aagcggaaat cacccacctt ccagttcatg ctgcaaagca agttgatcaa
     1981 cgacgttccc agcgtttttt cctcgttgtt ggcaatacgg ttctgtgcaa acacggtttg
     2041 gttctccaga ctggccgaat actggatcaa cgggcccttt ccgtgattgg aataaatcag
     2101 tgccaacaac atatccacag gtggtgaaaa aggcaacgga taaccgttgt cgatattccg
     2161 gttgtaaaca tattcccaat tggctgacaa caaaaggttc tcggtaaaac gataggccgc
     2221 ttccagctct ccacctgcca gaaacacacg ggactgggta tattcgtaaa tctgtccggc
     2281 atgtggcaaa accgaccatt gccccgtagg gttcaggtaa atataattgg ggaagtagga
     2341 tgcaaaatga ttgatggaga ggtttaatcg ccggttcttg agggaataac caaggtcata
     2401 ttgccaaccg gtttcgggtt tcaaactgtt gtttcctttc tcgtgacgga aagctccgtg
     2461 atgcaccccg ttggatgcca actcattggc agtaggaaag cggaagcttc gtccaatgtt
     2521 tatctttagt gaactcagtc ggtttgggtc atagaccgcc cccagcgccc cggagaacga
     2581 atgaaaattc tttcttaagg catcggcacg ctgggcgtaa taccagacat catcagcatc
     2641 atatccgcca agttgcagga aatcggccaa agtgtcgtcg tagaaaccgc gaatattcaa
     2701 tttcccggaa tcgaaacgca cccctccggt aagcatcagt ttcggggata ccttatacga
     2761 ggttgccgca tatgctccgg cggtatatct ttcaaagtca ggcatcagaa aagaataccc
     2821 tcccaccctg tttcgctgga cttccaccga catgcctgct gtttttgtcc agagcgaatc
     2881 tttgccgaaa gacagccggg agttgacgga cattgtttgc agtgagaaac ttaattccag
     2941 atcggggctg acttgtgggg gagcttggtt gccgtaatgc gtatggaact tcgaccattc
     3001 ggcacggtta ttttgctgaa aaccgatatc gaagtgcaaa gatgaattgt cccagtcgat
     3061 ttctgtcccg ttggagattt taaaatgatt ggaggtactg tatggatacc cgatattaaa
     3121 agaatttccg tcgggggtca gccttttcag atcagggata ccgtgtgaac cggggaaaaa
     3181 gccgtttttg gagaaaacat tggaaatatg ggtccacgag ctgatgtttt cctggctgta
     3241 gttggccgac aaagcagcgt ttagttcctg tccggctgtg tttttcattc ttcgtccata
     3301 aaggggcagt ttccacgtca ggtaggtaac cgtatcggcc ggaacgcgat aatcggcgaa
     3361 gcgttgtgcc gtcaggcgtc cacgtaaaaa ccaccttccc ctttttacgc ttgtcattgc
     3421 cgttattccc gacagattat tgttcgattt ggcaatcagg gacacatccc cccaaaccat
     3481 gttttccgaa gggacatcca ccggaaggat ctcaataaca ccaccaatgg catcggaacc
     3541 gtaaaaaagc gacatgggcc ctttgtgaat aaacacctta tccacgtcgt actggtcaat
     3601 gtcaagcccg tgatcggctc cccattgctg attttgctga acaacccctt tgtctgctac
     3661 tgctatgcgg ttgaaaccca atccgcggat aaccggtttc gacacgtttg cccctatatc
     3721 catcgatgaa acgccggcaa ccttcgataa ggattttgaa aagctggccg aattgttttt
     3781 catgaaaaag gacctgtcaa tttcttttgc agtgatggca gacaacaagc gaccatgacc
     3841 gtttttcccg tgcacgacta cttcgttcaa cgaataggag gttgtttcca ggtggaagtg
     3901 caacacagtg tcggaagtca ggatgatgct cgtcgttagt aactcgtatc cggtaaaacg
     3961 aacctccagg gtatgtgggg tttttccggg cagatcaacg gaaaaagccc cgtccctgcc
     4021 ggaaactgcg taaatttcat tcggccccag cactactatc gctccgggaa gcggctcctt
     4081 ggacaaggca tcgcaaacat accctttcag ggagtaagtc tgagaaaacg acaacaaaaa
     4141 acttaaggcg aaagtcatcg ccagaacaat ttttttcatt caggaggaag ttgttacatc
     4201 agttacaggc agcacagaca ccttctacaa tcatcgaaac acggtgttga acaaaccctt
     4261 gcggcaattc tatgtttatt tggcttttgt tttgaataca aaagacctcg ttgcaaacgc
     4321 agcaatgaaa atgggaatga atatcttgtt cgtggaaagg tttcttggaa acggcatacc
     4381 tgattgtgtt gtcctgcaaa acaatcttgt ggataacacc ggcgtcctcc aacgttttga
     4441 gcgttcgata aaacgtaaca cggtcaaaaa gatcgggaaa cgaagttttt atttcgtgtt
     4501 cagtcagagc cgtctggctt cgaagcaact cattgagaat gaacttccga caggttgtac
     4561 tttttaaatg gtaatcgcgt aaaatattct cgactgtcat aatgctataa ccttacaaat
     4621 gcaacattgt tgcaaatgta acactatttc attaaaatga ggtatccggg taatttaaaa
     4681 aatattaaaa tgatggatta cctggccctc tcaatccatt tatcggccag ttccgcatcg
     4741 tatcccaatt cttttgcctt ttcgaaatca actctggcgg caaacttgtc aaactgatcc
     4801 aaccgaagcc ggccgcgtaa aacataaaaa tagggatttg ccttttcgtc gctttcgaca
     4861 gcgtgaaggt ctgcggcggc tttcgagaac cggtcggtgt tcacgtaaca ttcggccctg
     4921 ttaaggtaat atgccgagct cggttttaca gttgtgttaa taatatcggt ataaattttc
     4981 tcggcttctg tccagttatc atcaagcttg aagatcaacg ctttgctgag cttggtgaac
     5041 aagttgccgg ggtccgtgct ttcgcaggtt ctgaaatcgg cttcggcact ttcccggtcg
     5101 tttttttcca ggaacagcaa tccccgacga taataggcct ccacgtctga cggttgttta
     5161 tcaataatgg agttgtaatc ctccatcgcc tcatcaaaac ggcccaagtc gcacaaaagg
     5221 gaagcccggt tatgaagcac caacaccgga tcggggttgt tcccaaggga tgccgtgaat
     5281 gaaatataag cgtcatcggt ctgcctgagc tgacgttgca ggattcccag attcaacagg
     5341 agcacagcat tattttcgtt agccggatcc agcctcatgg ctttttgcaa ggcaacagca
     5401 gcactgtcga gtcggttatt ttcgataaac tttgccgatt tgtcgatcca tccgttatag
     5461 gtatcggtct gggccgatat tgaaaaactg caaaacagca gcaagagaaa attcaaaata
     5521 tatttcattg tactacccga tcaaatgaac gacaaaatta gctcattttt tgtaactttg
     5581 cgccctcaat tgcatttaaa agaaataaac tatgttatta aatacttttg tatctgccga
     5641 ttctgcagta gtaaaaaccg gaggaaaagt tgccgagatg atcaaagatg gccgctttga
     5701 ggagttagga caacaatccc tcacgtgggg aatcgatttc ctgggtaaac ttgcggtagc
     5761 tctcctcatc ttttttgtag ggaaatggat aatcggtaaa atccgaaaac tggcagacaa
     5821 gatcatgtcg agacggcaaa tggatatcgc cctgaaaggt tttctaaaga acctgctcga
     5881 tatctttctc ttcgtactgc tcgttatgtt gatcataaac gtagtcggaa cacaaaccgt
     5941 ctctctggcg gcattgattg cttcggccgg cctggcggtt ggcttggcgg taaaagacaa
     6001 cctggccaac tttgccggag gcgtgatgtt gctcttcaac aagcctttca aaggcggcga
     6061 ctacattgaa gcgcagaacc tggcgggaac cgttcagagc atcggtattc tctatacaac
     6121 gctaaccaca gccgacaaca aaaccattta catacccaac gggccgctgt cgaccggaaa
     6181 catcatcaat tacaacacac aaaccacccg gcgggtagaa attacagcga gcattgaata
     6241 cggaaccgat gtggatatag taaaaaaaat gctgctggat attgccaaca ggcatccgtt
     6301 ggtgttgaaa gatcccgagc cgtttgcccg gatgaccaaa atgaacgaag attctatcga
     6361 tttctccctg agggcctggg tgaatttaaa cgactactgg aatgttaccc acgacctgaa
     6421 tgaggaggta tacaaagaga tcaatgcgcg cggcctggtg atccctttcc gtcaaatgac
     6481 cgtccatctg gccaactcaa ctgaacaaaa cgccaattaa tgcctgttac cgaaagaata
     6541 gccaagtacc gggagcttgc cgaaaatcag ctcaatgctg ctgccaggta caagaaagaa
     6601 ctcaatgcga acagtctttt acgattcttc gtctttttga ccggttcagc gcttacctat
     6661 ttttttcatc aaaatacttt gctggctgtt ctctttgccg cggtaaccgg agtttgtttc
     6721 ttggccctgc tggccaggca taagaattta ctgtttaaaa aagccaaatc agaagcgctg
     6781 gcccggatag ccgggaacga actccaggcc tttgtatacg atttctcgcc tttcgacggc
     6841 gcaccggagc atgtggatcc tgctcacagc ttcagctttg atttagatat ttttggggaa
     6901 aggtctgttt ttcaaatgct caaccgaacc agccttgcca tggggaaaga agctcttgcc
     6961 gggatagtag gaagtccgct taaggacagc ggagaaatcc gcataaggca gcttgctgta
     7021 aaagagcttt cggagaaaga agatttctgc ctcagcttca ggacaattgg gatgttatcg
     7081 gacgataaat ttttcgataa tcgttccatc aaacaacttt gtaggaaaca ggaactgttt
     7141 ccccggcctt cattatggaa aattgccacc tgggttgttc ctgctttata cgtactggcc
     7201 attgcactgg cgctggccgg actgacaagc ggagcggcaa tcaccttgtt gtattttaca
     7261 acattgttgg tttcggttat ccccatgaag aaggtgaagc agatccggtc tttattcgat
     7321 aacaaggcaa aagtgctgga atcgtatgcc gacctgttaa aattgatgga aacgaccagc
     7381 ttcgaaagtg tacagttaca ttcgctgcaa cagtcgataa agaacgaacg gcaaagtgcg
     7441 tcagctgcca ttaagcaact ggcacaatac agccggaacc ttgatctggc ttttgccgta
     7501 cccgtttttc ttctcctgaa tcctcttttt ttgtggaatg ttgtttacgc cctgaagata
     7561 gaacaatgga tgcaacgcca tgctgtggaa atcgaaaaat ggttctctac actggcacag
     7621 gttgatgcgc tagtgtccat gggcacgttt accgcaaaca accccgatta tgtgtttccc
     7681 acgtttgagg aatcactccc tttccgggca agaaaactgg ggcatccgct ccttccgcgc
     7741 cgtaaatgcg taaagaacga tatcaacgtt ttccggaaac cgtttttcat gattgttacc
     7801 ggagccaata tggccgggaa aagtacttat ttgcgtactg ccggtgtaaa ttacgtgctg
     7861 gcaggggccg gcctgcccgt ttgtgccgaa tccatgagtt atcgtccggg ccagttattg
     7921 acaaacctgc gcacagccga ttctctggta aacaacgaat cctatttttt cgccgagctc
     7981 aaacggctga aaatgattat cgaacggttg gagtcgggag aacacgacct gttcattatc
     8041 ctggacgaga tactgaaagg cacgaactct cacgacaagc aaa
//
LOCUS       DAOSDR010000095         8069 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrimonas sp. isolate AMR_MDS_1826
            NODE_3310_length_8069_cov_3.731844, whole genome shotgun sequence.
ACCESSION   DAOSDR010000095 DAOSDR010000000
VERSION     DAOSDR010000095.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863355
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrimonas sp. (wastewater metagenome)
  ORGANISM  Petrimonas sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Dysgonomonadaceae; Petrimonas.
REFERENCE   1  (bases 1 to 8069)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8069)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:10:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,720
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 35
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8069
                     /organism="Petrimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3310_length_8069_cov_3.731844"
                     /isolate="AMR_MDS_1826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2023866"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..416)
                     /locus_tag="PLI30_05215"
     CDS             complement(<1..416)
                     /locus_tag="PLI30_05215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019541025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HOI78989.1"
                     /translation="MVSDISQLLRILEVHPNVAAANKLLKEVKTLLVKGLHGSSRALF
                     SATLYNKQPRTFLFILNDLETAGYFYHDVTQILGTKDILFFPSAYKRAAKYGQMDPAN
                     EILRTEVLSRLQNNDSSLFIVSYPDALAEKTVSQATL"
     gene            569..2212
                     /locus_tag="PLI30_05220"
     CDS             569..2212
                     /locus_tag="PLI30_05220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019541024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch synthase"
                     /protein_id="HOI78990.1"
                     /translation="MDAKHTPDYIFEASWEVCNKVGGIYTVLSTKANSLQKLYKDKVF
                     FIGPDVLQPSDNPWFLEEKELYADWRNFARINQNLEIRAGRWNVPGNPIVFLIKFDRF
                     FEQKNSIYSEMWLDFGVDSMYAYGDYDESSMFAYASGVVIESFYRFHRLEACHVIAHF
                     DEWQLGMGALYIKKYVPKIATLFTTHATGVGRSIAGNGLPLYNHLKNYNGDQMARQLN
                     MVAKHSIEKRAAQSVDCFTTVSDITAQECSQFLQRQPDVVTPNGFEKNFIPNGLNYRR
                     TRKKARETLLSVAEKLLGHFVHPDALLVGISGRYEYKNKGIDVFIDALDTLRKMPQLS
                     KDVIAFIMIPAWIKGPREDWQSALYTTHQLQDIENDKIVNYLKYLGFSNSEQERVKVI
                     FVPSYLNGFDGIFNTDYYNLLIGLDVSVFPSYYEPWGYTPHESVAFSIPTITTTLAGF
                     GVWAKKNGDTEKGLSDGVQVIYRDDSNYKEVAEEIAATLYDFTLKSIDQVNVLKKMAA
                     ELSDRADWAHFITYYQEAYRKALHNSFIRLSKPARYKAD"
     gene            2368..4935
                     /gene="glgP"
                     /locus_tag="PLI30_05225"
     CDS             2368..4935
                     /gene="glgP"
                     /locus_tag="PLI30_05225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008141500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucan family phosphorylase"
                     /protein_id="HOI78991.1"
                     /translation="MIIKASYSNTPVWHDVHVHSILPEELRPLEEIAHNLWWVWSEEA
                     KEIFELLDYEEYEKCGKNPVALLQNLRTEKTEEILKNADLMARIGRLHQSYKNYIGTP
                     FDADRPSIAYFSMEYGLSHALKIYSGGLGVLAGDYLKEASDSRVDMTAVGFLYRHGYF
                     TQTLSVDGQQIANYEAQNFGSLPITQVLDEHNKPVVLEVPFHDRTIYSNIWKVSVGRI
                     QLYLMDTDLEHNSEYDRSITYQLYGGDWENRMKQEYLLGVGGILLLKRLGIRKDVYHM
                     NEGHAALISAKRLRDYVQEEKLSFNEALEVVRASTLYTVHTPVPAGHDYFEESLIRKY
                     MEPLVNKIGIPWYQFMDMGRDNPGTNEKFSMSVFALNTAQESNGVSKLHGLVSQEMFQ
                     PVWKGYFPQELHVGYVTNGVHLPTWATSSVKRIYENNLGEDFYQDQSNPEIWKKVYDI
                     SDEEIWGLRMHLKEKLVDYIKSEFVDGWLKNQAAPSRIISMLDEIRPGSLIIGFSRRF
                     ATYKRAHLLFSDLDRLAKILNNPERPVTFIFAGKAHPADGGGQSLIKYIVEISRRPEF
                     LGKIIFLENYNMRLAKRLVSGVDIWLNTPTRLQEASGTSGEKAIMNGVLNLSVLDGWW
                     YEGYVEGAGWALTAKRTYENQAFQDELDASTLYTMLESEIIPLYYAHNSNGYSPQWVK
                     FVKKSIAEIAPHYTTKRMMDDYFDRFYMKLAQRSKKLAENNYAKAKEIVRWKEDTASK
                     WDKIEVIKLEFEPVQEVDINNGKNKIYGEVVIDKKDIAAELGLECVVVDYDSTANKVE
                     FIEKYEFNLLKTEGSRLFFQTREALNDPGTHQYALRIYPKNPDLPHRMDFAYVKWIS"
     assembly_gap    5016..5115
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(5252..5707)
                     /locus_tag="PLI30_05230"
     CDS             complement(5252..5707)
                     /locus_tag="PLI30_05230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019541002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HOI78992.1"
                     /translation="MVDKYVFSDVSTCTFNFSDGKRAFVKSSPRKEIVLVKFIPHTSK
                     KNSFQESVVANYPESKNVSELAQKCNYDCLKTFTRHFKKHFNQTPYQWMLDRKMEEVQ
                     HLVLESDLSISDIAKICGFNNLTHLVNAYSSKFGIPPFRNRMQENKNAI"
     gene            complement(5729..6124)
                     /locus_tag="PLI30_05235"
     CDS             complement(5729..6124)
                     /locus_tag="PLI30_05235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI78993.1"
                     /translation="MKTKFFYTMALICSTLFFSSFAVTETNKVNDQQDKIVFSKTGGT
                     TRGVTGVEVAATTDGKTTVAISVVDFTGTAVVQIIGGRTSAQYYFDVNEMGYEVINIG
                     TLRAATYTIRIILDNEVYEGTFTKEAVGR"
     gene            complement(6306..7985)
                     /locus_tag="PLI30_05240"
     CDS             complement(6306..7985)
                     /locus_tag="PLI30_05240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI78994.1"
                     /translation="MRCFIYIAIAAAAAVLFISCNERENGEIHKILTQWDNLLEKNPN
                     AISDSLKTIDPQTLSRENRAYYNLLKTISDDKTYFEFTSDSLITRVADYYHLHEPYSN
                     NYIRALTYKGIVRTRMGITDSTVYEPLKKADHIFHTYKNPDPATGYILHYFLGNTHYY
                     NGNFELAEKYFQETLYNAKLKNDSLHIFDTYLAIYWNEMAQSDFDAGKRYLDTLTNFA
                     SMSSEANYFVLNAKSVFHESMGNYTDALECVKKQLEVSKKLKENIDISSLYYIVSDRY
                     KNLNYLDSAMLYGQIAIENIVDEANKGNYLLYENVANIAEKQQNYLLANTYRQKAFDL
                     YEKSVKDRLNTQVMELEKKYDLSEAENIALRSQQNTLIIAIIALLLAMLATILIMLNV
                     KNRREAKIKLMRLEHEAETQAIKTKLLEEEANKRDWLIQLYGYISNRLTSLQENFNTL
                     SQRYVSSHPKIYENMHNILHTAESDLREMPKNLIPDENTFSLYTNLSMEAAELFNANE
                     KIMLMLLVCKADNRQISTFMNTSIESIRARKSQLKKKLTENGIDAAHFFNF"
BASE COUNT         2247 a         1554 c         1821 g         2347 t
ORIGIN      
        1 agtgttgcct gagaaaccgt tttttctgcc agcgcatcgg ggtaagatac gatgaacaac
       61 gaagagtcgt tgttttgtaa acgactcaac acttcggtgc ggagaatttc gtttgccgga
      121 tccatctgtc cgtacttggc agcacgtttg taagccgaag gaaagaaaag gatgtctttc
      181 gttcccagga tctgtgtaac gtcgtgataa aaatatcctg ccgtttccag gtcattgagg
      241 ataaaaagaa aggtccgcgg ttgtttgtta taaagcgttg ccgagaaaag tgcacgcgaa
      301 gaaccgtgca aacctttcac taaaagtgtt tttacctctt tcagcaattt gtttgcggcc
      361 gctacgttgg ggtgaacctc aaggatccgg agcaattgag agatgtctga taccatcgtt
      421 tataaattcg gggcaaaggt acaaagaaat tacgatttta gatttacgga ttgtgcctgt
      481 gattatgata tttttaagac ttcccttttc gctttttcac tttaaatctg tatctttgtc
      541 tttcaaatta cagttctttt atatgcaaat ggatgcaaag catactcccg actacatctt
      601 cgaagcaagt tgggaagtgt gtaacaaagt tggcggtatc tacaccgttt tatcaacgaa
      661 ggcaaattca ctacaaaaat tatacaaaga caaggttttt tttatcggcc ccgatgtttt
      721 acaaccatcg gataatccct ggttcctgga agaaaaggag ctttacgccg attggagaaa
      781 ctttgcacgg ataaaccaga acctcgagat acgtgctgga aggtggaatg tacctggaaa
      841 tcccatcgtt tttctgatta agtttgaccg gttttttgaa caaaagaaca gtatctattc
      901 agagatgtgg ctcgattttg gggtagactc catgtatgct tacggagatt acgacgagtc
      961 atcgatgttc gcctatgctt cgggggtggt tatagaaagt ttctatcggt ttcaccgttt
     1021 ggaggcgtgc catgtcattg cacacttcga tgagtggcag ttaggtatgg gagccttgta
     1081 catcaagaaa tatgttccga aaattgctac gcttttcacc acacacgcca cgggagtagg
     1141 acgctctatt gccggaaacg ggctcccgct ctacaatcac ctgaaaaatt ataacggcga
     1201 tcagatggcg cgccaactga atatggtggc caagcattcc attgagaaaa gggcggcaca
     1261 aagtgtggat tgtttcacta cggtaagtga tattacggca caagagtgtt cgcagtttct
     1321 tcagcgtcag cctgatgtgg tgactcccaa cggatttgag aaaaacttta tccccaacgg
     1381 actgaactat aggcgtaccc gaaaaaaagc acgcgaaacg ttgctgagtg ttgcggagaa
     1441 acttctgggg cattttgttc atcccgatgc actactggta ggaattagcg gaaggtatga
     1501 atacaagaac aaaggtatcg atgtttttat cgatgcgttg gacacgttgc gcaagatgcc
     1561 tcaattgtcc aaagatgtga ttgcattcat tatgatccct gcttggataa agggtcccag
     1621 ggaagattgg caatcggcac tttatacaac tcatcagctg caggatatcg aaaacgacaa
     1681 gattgtaaat tacctgaaat accttggatt tagcaattca gagcaggaga gggtaaaagt
     1741 aatttttgtt ccgtcttacc tgaacggatt cgacggtatc ttcaatacgg attattacaa
     1801 cctgttaatt ggcctggatg tttctgtttt tccatcctat tacgaaccgt gggggtacac
     1861 accgcacgag agcgttgcct tttccattcc taccatcacg acaacgctgg cgggttttgg
     1921 tgtttgggca aagaaaaatg gagatacaga gaaaggttta tccgatgggg tacaggtcat
     1981 ttaccgggac gacagcaact ataaagaggt ggcagaggag attgcagcca ccctatacga
     2041 ctttacactc aaaagtatag atcaggtgaa tgtgctgaaa aaaatggctg cggaactttc
     2101 cgacagggca gattgggcac atttcatcac ctattatcag gaagcttatc gcaaagcatt
     2161 gcacaactct tttatacggt tatcgaaacc ggcaagatac aaagccgatt aaatgcttat
     2221 atttgcggaa aagaacaaaa acttttagac gattgggtag tcagatggtc agactatcct
     2281 gacttttaaa agcgtcggtc tgaaattctg atgatttgaa ttcagacaaa aatcaacatt
     2341 ataaaatcaa caattaaacg ataaaaaatg attattaaag cgagttattc aaacactccg
     2401 gtatggcatg atgtgcacgt acattcaatt ttgccggaag agttaaggcc gttggaagaa
     2461 atcgcccata acctttggtg ggtttggagc gaggaggcaa aagaaatttt tgaattgctg
     2521 gattatgagg aatacgaaaa gtgtggtaaa aatccggtag cgctacttca aaacttgcgt
     2581 actgaaaaaa cggaggaaat tttgaaaaat gctgatctga tggctaggat aggtcggcta
     2641 catcaatcat acaaaaacta tataggtact cctttcgatg cagaccggcc cagtatcgcc
     2701 tatttcagca tggaatacgg cttgtctcac gcgttaaaga tatactccgg cgggctcggc
     2761 gttctggccg gtgattacct gaaagaagca agcgacagcc gggttgacat gactgctgtc
     2821 gggttcctgt accgccacgg atatttcacc caaacccttt ccgtggacgg gcagcaaatt
     2881 gccaactacg aagcccaaaa ctttggttcc ttacccataa cccaggtact ggatgaacat
     2941 aacaagcctg ttgttctcga agtacctttc cacgacagaa ccatatattc aaatatatgg
     3001 aaagtatcgg tagggcgtat tcagttgtat ctgatggata cagatcttga acataacagc
     3061 gaatatgacc ggtccataac ctaccaactt tacggagggg attgggaaaa ccgcatgaaa
     3121 caggaatatt tgcttggcgt aggaggaata ttgcttctga aaaggctagg aatccggaaa
     3181 gatgtgtatc acatgaacga ggggcatgca gcccttatca gtgctaagcg gttacgcgat
     3241 tatgtgcagg aagaaaaact gtcgttcaac gaagcgttgg aggttgtacg tgcatccacc
     3301 ctttatacgg tacacacccc tgttcctgcc ggtcatgatt attttgagga gtcgcttatc
     3361 cggaaataca tggaaccttt ggtcaacaag ataggtatcc cctggtatca gtttatggat
     3421 atgggacgtg ataatccggg tacaaacgaa aagttctcga tgagtgtttt tgcgttgaac
     3481 accgcacagg aatcaaacgg agtaagtaaa cttcacggcc tggtttctca agagatgttc
     3541 cagccggtct ggaagggtta ttttccacag gaactccatg tgggctatgt taccaacggt
     3601 gtccacctgc cgacctgggc aacttcatcg gtaaagagga tttacgagaa taacctgggc
     3661 gaagattttt accaggatca gtctaatccc gagatatgga aaaaggtgta tgatatcagt
     3721 gatgaggaga tttggggatt acgcatgcac ttgaaggaga agcttgtcga ttacatcaag
     3781 agtgagtttg ttgatgggtg gttaaaaaat caggctgccc cctcccggat tatctctatg
     3841 ctcgacgaaa tcaggccggg ctctttgatc atagggtttt cgcgccgttt cgccacctat
     3901 aaacgggctc atttattgtt ttcagattta gaccgtctgg caaaaatact gaacaatccc
     3961 gagcgtccgg ttacgtttat ttttgccggc aaagcgcatc ctgcagatgg tggtggacaa
     4021 tcgctcatca agtacattgt ggaaatatcg cgccgccccg aatttttagg aaaaataatt
     4081 ttcctcgaga actacaacat gcgtttagcc aaacgtttgg tttcgggcgt tgatatctgg
     4141 ctgaacactc ctacgcgttt acaggaagct tcgggcacat cgggtgaaaa agccattatg
     4201 aacggcgtgt taaacctttc tgtattggat ggctggtggt acgaggggta tgtcgaaggg
     4261 gccggttggg cgttaaccgc aaaacggact tacgaaaatc aggctttcca ggatgaactg
     4321 gatgcatcga ctctttacac catgcttgag tcggagatta tcccgcttta ttatgcgcac
     4381 aacagcaacg gatattcgcc tcagtgggtg aagttcgtga agaaatcgat tgcagaaata
     4441 gccccgcatt acacgaccaa acggatgatg gacgactact tcgaccgttt ttatatgaag
     4501 ctggcacaac gatcgaaaaa gcttgccgaa aataattatg cgaaagctaa ggaaatcgtt
     4561 cgttggaaag aagataccgc atctaaatgg gacaagatcg aagtaattaa actggagttt
     4621 gagccggtac aggaagtaga tataaataac ggaaaaaaca agatttacgg cgaagtggtt
     4681 atcgacaaga aagatattgc tgccgagttg gggctggagt gcgtggtggt tgactacgac
     4741 tcaactgcca ataaggttga atttattgaa aaatacgagt tcaacttgtt gaaaacggaa
     4801 ggttcccgac tttttttcca aaccagagaa gcgctaaacg acccaggcac acaccagtat
     4861 gctttgcgta tttatccgaa aaatccggat ctgccgcacc gcatggactt tgcttacgtg
     4921 aagtggatca gttaaaagaa aacgccccag aatggggcgt ttttgcattg atttcatcca
     4981 caatgaagtt tagttgagtt tataagaaga atagtnnnnn nnnnnnnnnn nnnnnnnnnn
     5041 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5101 nnnnnnnnnn nnnnnttttg cattgatttc atccacaatg aagtttagtt gagtttataa
     5161 gaagaatagt tcgtacgggt tttgtataac cggacgtttt ttttgtttca tgaaaattgc
     5221 gtaacaataa ctactccttt ttgcaaatta atcaaattgc atttttgttt tcctgcatcc
     5281 tgtttctgaa aggcgggatg ccgaacttgc tggagtaggc attcaccaag tgtgtcaggt
     5341 tgttgaaacc gcatattttt gcaatatcgg atatcgacag atccgactcc aataccaggt
     5401 gctgtacttc ttccattttg cggtcaagca tccattgata aggcgtttga ttgaagtgct
     5461 ttttaaaatg tcgggtaaaa gtcttcagac aatcgtagtt gcatttttgt gctaattcgc
     5521 tgacgttttt ggattcggga taattagcga ccacactttc ctgaaatgag ttctttttcg
     5581 aggtgtgggg gatgaatttt accagtacaa tttcctttct gggcgatgat tttacaaagg
     5641 ccctttttcc gtctgaaaaa ttaaacgtac aggtacttac gtcggaaaat acatatttat
     5701 ctaccatggc ttctcttgtt ttaatggatt acctacctac tgcttctttt gtaaaggtgc
     5761 cttcataaac ctcgttgtcc aaaatgatgc gaatggtata tgtggctgcc ctcaatgttc
     5821 caatgtttat cacttcgtag cccatttcgt tcacatcgaa ataatactgt gccgaagttc
     5881 ttcctccgat tatctgcact acggccgtgc cggtaaaatc caccacagat atggcaacgg
     5941 ttgttttgcc gtcggtagtc gcagctactt ctacgcctgt taccccgcgg gttgttcccc
     6001 cggttttact aaatacaatc ttatcctgct gatcgtttac cttgtttgtt tcggtaacag
     6061 caaaagaaga aaagaaaagc gtgctgcaga ttaacgccat cgtataaaag aattttgttt
     6121 tcataggagt aaaatttatt atgaattaca gacacaaaat aataaacaac agacaagaaa
     6181 aacaataccc aggtcgaatt ttaacggtag attataggtt aaataattga tagtaagcat
     6241 attgatcgga ttgattttaa cgctaccaaa ggtggttgaa acgatgattt tgcaagaaat
     6301 gtgtgctaaa aattgaaaaa atgagcggca tcgatcccgt tttctgtcaa ttttttcttc
     6361 aattgcgatt ttcttgcgcg aatactttct attgaagtat tcataaaggt ggatatttgc
     6421 ctgttgtctg ccttgcagac caacagcatc aacatgatct tctcgtttgc attgaaaagt
     6481 tccgcggctt ccattgataa attggtgtat aaagaaaatg tattctcgtc gggtatcagg
     6541 ttcttgggca tttccctcaa atcggattcg gccgtatgta atatgttgtg catgttttcg
     6601 tatatcttag gatgtgaaga aacataccgt tgcgacaggg tattgaaatt ttcctgtagc
     6661 gacgtaagcc tgtttgaaat gtacccatac agctggataa gccagtccct tttgtttgct
     6721 tcttcttcca gcagctttgt tttgatggct tgtgtttcgg cttcgtgctc caaccgcatc
     6781 agttttattt ttgcttcgcg gcggtttttt acgttcagca taatcaaaat agtggcaagc
     6841 atagcgagca acagggctat aatagcaata ataagcgtgt tctgttgaga tcttaacgcg
     6901 atattttcgg cctcggatag gtcgtacttt ttttccagct ccataacctg ggtatttaac
     6961 cggtctttca ccgatttttc atacagatca aaagcttttt gccggtacgt attggccaaa
     7021 agataattct gctgtttttc agcaatattg gcaacgttct cgtacaataa ataattacct
     7081 ttattggctt catcaacaat gttttcaata gctatttgcc cataaagcat ggcactgtct
     7141 aaataattca aatttttgta tctgtcggaa actatatagt acaagctgga tatatcaatg
     7201 ttttctttta acttcttgga gacctccagt tgttttttta cacattccaa agcatccgta
     7261 tagtttccca ttgattcgtg gaacacagac tttgcattta atacaaaata atttgcttct
     7321 gaagacatac tggcgaagtt tgtcagagtg tctaagtacc tttttcctgc atcaaaatcg
     7381 gattgagcca tttcattcca atagatagcc aaataagtat caaagatgtg caatgaatcg
     7441 tttttcagtt tggcattata caatgtttcc tgaaaatatt tttcggccaa ttcaaaatta
     7501 ccgttataat aatgcgtatt tcctaaaaag tagtggagga tgtatccggt tgccggatcc
     7561 gggtttttat aggtgtgaaa aatgtgatcg gcttttttta aaggttcgta tacggtgctg
     7621 tctgttattc ccatgcgggt acggacaatg cccttatacg tgagggcgcg gatatagttg
     7681 ttgctgtagg gctcgtggag gtgatagtag tctgctaccc tggtaatcag ggagtcgctt
     7741 gtgaattcaa aataggtttt atcgtccgag attgttttga gcaggttata ataggcgcgg
     7801 ttttcccgcg aaagggtttg tggatcaata gttttcaggc tgtctgaaat agcgttggga
     7861 ttcttttcca acaggttatc ccattgtgta agtattttgt gtatttcacc attttcccgt
     7921 tcgttgcaag aaatgaaaag cactgcagca gccgccgcaa tggcaatata tataaaacag
     7981 cgcattttct tgaagtttat ttacggcaaa gataattttt tttgttgaat ttgtttaata
     8041 aaaaggacaa gattcagatt taatgttgg
//
LOCUS       DAOSDR010000096         8034 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrimonas sp. isolate AMR_MDS_1826
            NODE_3322_length_8034_cov_3.855997, whole genome shotgun sequence.
ACCESSION   DAOSDR010000096 DAOSDR010000000
VERSION     DAOSDR010000096.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863355
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrimonas sp. (wastewater metagenome)
  ORGANISM  Petrimonas sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Dysgonomonadaceae; Petrimonas.
REFERENCE   1  (bases 1 to 8034)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8034)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:10:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,720
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 35
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8034
                     /organism="Petrimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3322_length_8034_cov_3.855997"
                     /isolate="AMR_MDS_1826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2023866"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..261)
                     /gene="nrfA"
                     /locus_tag="PLI30_05245"
     CDS             complement(<1..261)
                     /gene="nrfA"
                     /locus_tag="PLI30_05245"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007659437.1"
                     /note="catalyzes the formate-dependent reduction of
                     nitrite to ammonia; cytochrome C552; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-forming cytochrome c nitrite reductase
                     subunit c552"
                     /protein_id="HOI78995.1"
                     /translation="MSSNDNRKMKPWVAWLLFFVTVGIVFLLGMLAASITQRRAEVVS
                     ILNNKKVNITGIESRNEIFAENYPREYESWIKTADTTFQSEFN"
     gene            complement(288..893)
                     /gene="nrfH"
                     /locus_tag="PLI30_05250"
     CDS             complement(288..893)
                     /gene="nrfH"
                     /locus_tag="PLI30_05250"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019538853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase small subunit"
                     /protein_id="HOI78996.1"
                     /translation="MGKLTHIWNEIKPRGGWKYPAIIIVGAFAGLFIYTFFTSRAYSY
                     LSDNPRTCVNCHVMSPYYATWTHSSHARNTTCNDCHVPHNNIVNKYFFKAKDGLRHSA
                     VFTLRGEKTALQTIEASSEVIMDNCIRCHTQLNQEFVNTGRMGYKEMKEMGGNACWDC
                     HRDVPHTRSRSEVSTPHSHVPMPDTHVPDWLSTAMKRNQIK"
     gene            1087..1701
                     /locus_tag="PLI30_05255"
     CDS             1087..1701
                     /locus_tag="PLI30_05255"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HOI78997.1"
                     /translation="MEERADFLRDVRYSIKRYGKGDMLVAQGSVYEMLYIVVKGEVVT
                     EMSDEKGEFVKMELIRAPNPLATGFLFATDNISPVTALAKTDCVVILIPKDNVFLLMS
                     KYRDFMKAFLAYISNKVAFLSEKLRLASLRTIRAKLGYYLLKESRGEQEFQLKMSKEE
                     LSRLLGVSRPALVNVMMQMADEGLIGVDRRKILIKDRIALQRLF"
     gene            1719..2492
                     /locus_tag="PLI30_05260"
     CDS             1719..2492
                     /locus_tag="PLI30_05260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007661982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HOI78998.1"
                     /translation="MKQLLIILVFTLVSSAAFAQQEEYQKKTYVSSENDTLLYRELVP
                     EKLILNQSYPLVIFLHGAGERGNDNEAQLRHGGNMFTNPVNREKYPAFVIFPQCPQED
                     FWAPRNRSAAAGENPFNQNPPVSKALSLVKKILDEAIEKYPVDRNRIYVAGLSMGGMG
                     TFDMLCRYPDLFAAAIPICGGVSTERLRKIKTKTNIRIFHGDADTVVPVTYSREAYKT
                     LKQNRASVEYIEYPGVNHDSWTPAFNEDDFMAWLFSKRK"
     gene            2575..4092
                     /gene="zwf"
                     /locus_tag="PLI30_05265"
     CDS             2575..4092
                     /gene="zwf"
                     /locus_tag="PLI30_05265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015531268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HOI78999.1"
                     /translation="MDNQALVIFGASGDLTYRKLMPSVFDLYMNNSLPDGYAVLGVSR
                     SKFTDSEFRKKMKEGIKEFSHFKNAGAEKINHFLKKVFYISIDTQKSEDYELVKSKLR
                     ELNKKLDLRHNYIFYLSTPPQLYPVIPKYLYGQGLTLQQKGFRRIIIEKPFGHDLKSA
                     MELNKSLTDFFSEEQIYRIDHYLGKETVQNLLVTRFSNGIFEPLWNRNYVQHVEITAS
                     ESIGVEGRGGYYETAGAMRDMVQNHLLQVASLVAMEPPAKVTSEEIRHEKLKVFRSLR
                     PLNEEDLRNNVIRGQYTESHVKGKYLTGYRDEEGVDKNSRTETYFAMKFFIDNWRWSG
                     VPFYIRSGKRLPTRVSEIVIHFKPSPTQLFKSVDGAIEAGNQLILRIQPDEGILLKTG
                     MKVPGNGYEVKSVNVDFHYSDLKDHYIPEAYERLILDCMTGDNTLYMSGEGAELTWRF
                     VQPILDYWESDPDAPLYGYPSGSWGPDSADKLIEGAQTWRYPCKNLADDGVYCEL"
     gene            4122..4841
                     /gene="pgl"
                     /locus_tag="PLI30_05270"
     CDS             4122..4841
                     /gene="pgl"
                     /locus_tag="PLI30_05270"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019538746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017057 - 6-phosphogluconolactonase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HOI79000.1"
                     /translation="MQTKIFNTKEELNRAFTDYLKEILKDRERVTVALSGGSTPKSLF
                     DYWAQRHATDIDWKSVFLFWGDERCVPPTDEQSNYKMTRDHLLDHVAIPERNIFRIKG
                     ENDPAAEAQRYSTLLQMEVETVDTVPSFDLVILGLGDDGHTASIFPHEIDLWESADLC
                     VVGTHPDSGQKRISLSGKVINHAQRVAFLVTGDGKAEKVKQIIESGKEVAAAYPAAKV
                     KPLTNNLVWFLDKEAARLLGK"
     gene            complement(4847..5404)
                     /gene="def"
                     /locus_tag="PLI30_05275"
     CDS             complement(4847..5404)
                     /gene="def"
                     /locus_tag="PLI30_05275"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013617680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HOI79001.1"
                     /translation="MILPIYVYGHPLLRKTAKEIDAATYPNLKELIDNMYETMYSADG
                     VGLAAPQVGLEDRIFVVDLEPLADNEHPEFKSFKKVFINAEITEKTGELEWVEEGCLS
                     IPGIHEKVPREERIRIQYLDEDLQPHDEEYTGYMARVIQHEYDHLDGIMFTDRISPLR
                     KRMIKSKLSNMEKGKVSCHYRVKTV"
     gene            complement(5421..5855)
                     /gene="ruvX"
                     /locus_tag="PLI30_05280"
     CDS             complement(5421..5855)
                     /gene="ruvX"
                     /locus_tag="PLI30_05280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006843792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HOI79002.1"
                     /translation="MSRLLAIDYGKKRTGIAVSDPLQLIANGLTTVDTAGIFDFLRSY
                     FEKEKVEKVIIGLPRQMNYEASENLPKIEQFADQLRKLYPHLDVVCFDERFTSKMAQQ
                     TMIDGGLKKKDRRNKALIDEISATIILQGYMESLIYKKERRQ"
     gene            complement(5852..6640)
                     /gene="lptB"
                     /locus_tag="PLI30_05285"
     CDS             complement(5852..6640)
                     /gene="lptB"
                     /locus_tag="PLI30_05285"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007557897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HOI79003.1"
                     /translation="MNDETRQAEVPDGQLVLRTENLVKKYGKRTVVSHVSIHLKQGEI
                     VGLLGPNGAGKTTTFYMTVGLIVPNEGSIYLGDQNITKYPVYKRAQYGIGYLAQEASI
                     FRKMTVEDNIKSVLEFTNKTPEERKKKLEELITEFGLEKVRKNLGDRLSGGERRRSEI
                     ARCLAIDPKFIMLDEPFAGVDPIAVQDIQSIVAQLKYRNIGILITDHNVDETLSITDR
                     AYLLFEGRVLFQGTAEELAENPVVREKYLGRDFELRRKNFEMLP"
     gene            complement(6729..7061)
                     /gene="folB"
                     /locus_tag="PLI30_05290"
     CDS             complement(6729..7061)
                     /gene="folB"
                     /locus_tag="PLI30_05290"
                     /EC_number="4.1.2.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008152389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HOI79004.1"
                     /translation="MRFYAYHGVLPQEKIIGNNFVVNVTFTAEVSRSFETDNVADTVN
                     YAAVYDLVKAEMAVPSKLLEHVAGRIFSEIKRSFPRINSLEVRLAKLNPPVSGEMEAS
                     EIIITDNE"
     gene            7171..>8034
                     /locus_tag="PLI30_05295"
     CDS             7171..>8034
                     /locus_tag="PLI30_05295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019538760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family N-terminal
                     domain-containing protein"
                     /protein_id="HOI79005.1"
                     /translation="MSTSTISERLIAIRNFMEAAKLDAFIIPSTDAHLSEYPPKRWES
                     RKWISGFTGSAGTAVVTKKRAGVWTDSRYFLQAADELEGTGFDLFKMGQPGTPEMTAW
                     IVEQVGKGGTVGIDGLVYAASDAKALRSALEAKDIRLETTLDPFAEVWKDRPEIPGNK
                     VFLLSEEITGESTKSKIERIHDELDRLGADGLIAVTLDAVAWIFNMRGSDVDFNPVAV
                     AYGYVSKKESVLFVDEEKLDDLTKNTLLKQGVKIDDYDRVFSYVANLPENTAVCLTGH
                     KINYKLYQTLSA"
BASE COUNT         2188 a         1667 c         1872 g         2307 t
ORIGIN      
        1 gttaaattca ctttgaaagg tggtgtcagc ggtttttatc caggattcgt attcacgcgg
       61 gtagttttct gcgaaaattt cgtttctcga ctcaatgccg gtgatattca cttttttgtt
      121 gtttaggata cttaccactt cagccctgcg ttgagtgatg gatgcggcaa gcattcccaa
      181 taaaaagact attccaaccg taacgaagaa taaaagccag gctacccagg gtttcatctt
      241 tctgttatca ttactactca tagggataaa tttgcgggtt atttatttta ttttatctga
      301 tttcttttca tggctgtact tagccagtct ggcacatggg tgtctggcat cggtacatgt
      361 gaatggggag tggacacttc cgagcggctt ctcgtgtgag gaacatccct gtggcaatcc
      421 cagcaggcat ttcctcccat ttccttcatc tctttatagc ccattcttcc ggtatttaca
      481 aactcctgat taagctgtgt gtgacaacgg atgcaattgt ccatgatgac ctcagaactg
      541 gcttctatgg tttgcagggc tgtcttctcg cctctcagtg taaaaacggc ggagtgacga
      601 agcccatcct tggctttaaa aaaatactta ttcacgatat tgttgtgcgg aacatggcag
      661 tcgttacagg tggtgttccg ggcgtgtgaa ctgtgggtcc aagtggcgta ataaggactc
      721 atcacgtggc agttaacaca ggttctgggg ttgtcggata aataactgta ggcgcgagag
      781 gtgaaaaaag tatagataaa caaccctgca aacgctccca cgatgatgat tgccgggtat
      841 ttccagcctc ctctgggttt tatctcgttc caaatgtgcg ttagttttcc catgataatt
      901 tagcttgaaa ctagagtttc tccacttcaa tacaaaaaaa gcgaatttaa ctgtaaaaga
      961 atgtaatatt tattacgaat tgagaaaaaa tagttatatt cgctaattac aattaatcct
     1021 atgaaccctc aacattcaga gttttttcaa actatttttc aatgcccgct gtgcaattct
     1081 attcctgtgg aagaaagagc cgacttttta cgcgacgtac gttatagcat aaaacgatac
     1141 gggaaaggag atatgctggt agcgcaagga tcagtttatg agatgcttta catcgttgta
     1201 aaaggtgaag tcgtcacaga gatgtctgat gagaaagggg agttcgtaaa aatggaactg
     1261 atcagggccc ccaatcccct ggcaaccggt tttttgtttg ctaccgacaa tatttcaccg
     1321 gttacagcat tggcgaaaac cgattgtgtg gtaattctta ttcccaaaga taatgtgttt
     1381 cttttgatga gcaagtaccg ggattttatg aaagcgtttt tagcgtatat ttcgaacaag
     1441 gtagcttttt tatcggaaaa gttaaggctt gcatcgttgc ggactatacg tgccaagctg
     1501 gggtattacc ttttgaaaga atcgaggggt gaacaggagt ttcaattgaa gatgtcgaaa
     1561 gaagagttgt cgcgcttgtt gggtgtttca cggccggcat tggtaaatgt tatgatgcag
     1621 atggcggatg aagggctcat cggcgtggat agacgaaaaa tcctgataaa agacaggata
     1681 gccttgcagc gcttgtttta aattatttaa atgtcaatat gaaacaatta cttattattc
     1741 tcgtattcac attggtctct tccgctgctt ttgcgcagca agaagagtat cagaagaaga
     1801 cgtatgtttc ttccgaaaac gatacgttgc tctacaggga gttagtgcct gaaaaattga
     1861 tactgaacca aagctatcct ttggtgattt ttcttcatgg tgcgggagaa cgtggaaacg
     1921 ataacgaagc acaactgcgt cacggtggaa atatgtttac aaatccggtg aatcgggaaa
     1981 aatatcccgc ttttgtgatc tttcctcaat gtccccaaga agacttttgg gcaccaagaa
     2041 accgttcggc ggccgcagga gaaaaccctt ttaatcagaa tcctcctgtg tctaaggcgt
     2101 tgagcctggt aaaaaaaatt ctggatgaag cgattgagaa atatcctgtg gatagaaaca
     2161 gaatttacgt agccggactt tcgatgggcg ggatgggaac atttgatatg ttgtgccgat
     2221 accctgatct ttttgctgcc gcaatcccca tctgcggggg agtaagcacc gaaaggctga
     2281 ggaaaattaa aaccaaaaca aatattcgaa tttttcacgg tgatgccgat acagttgtcc
     2341 cggttaccta ttcccgtgag gcatataaga ctctcaagca gaacagggct agcgttgaat
     2401 atattgaata tccgggtgta aatcatgaca gttggactcc tgcattcaac gaagatgatt
     2461 tcatggcatg gcttttcagt aaaagaaagt gaagattgac tcttttgaaa aagcaacttt
     2521 tccgccggtc gtttgtttac actctgtaga aaatagtatt taacaatgaa aaagatggac
     2581 aatcaggcac tggttatttt tggtgcctcc ggtgatctta cctatcggaa actgatgcct
     2641 tcggttttcg atctgtatat gaataattca cttccagatg gttatgccgt tttgggcgtt
     2701 agtcgctcaa aatttaccga ttccgaattt cgaaaaaaaa tgaaggaagg gatcaaggag
     2761 ttttcacact tcaaaaatgc aggtgcggag aagataaacc actttttaaa gaaagttttt
     2821 tacatcagca tcgacacaca aaagagtgaa gattatgaac tggtaaaaag taaactgagg
     2881 gagttgaata agaaattgga tctccgccac aactatattt tttacctttc cacacctccg
     2941 caactttatc cggttattcc aaaatatctt tatgggcaag ggcttacgtt acaacaaaag
     3001 ggattccggc ggattattat tgaaaaacct ttcggacacg atttaaagtc agctatggag
     3061 ttgaataagt cgctgaccga ttttttcagc gaggaacaaa tttatcgtat tgatcattat
     3121 ctgggaaagg aaaccgttca aaacctgctg gttactcgtt tttcaaacgg gatttttgaa
     3181 ccgttgtgga acagaaacta tgtgcagcac gtggaaatta cggcttccga aagcatcgga
     3241 gtggaggggc gcggaggata ttacgaaact gctggcgcta tgcgtgatat ggttcagaat
     3301 cacttgcttc aggtggcatc actggtagcc atggagccgc cggcaaaagt gacttctgaa
     3361 gagatccggc acgagaaact aaaagttttc cgttcgttgc gtccgttgaa cgaagaggat
     3421 ttgcgaaaca atgtaatccg gggacaatat accgagtcgc atgtgaaagg aaaatacctt
     3481 accggatacc gcgacgaaga aggtgtagat aaaaattcgc ggacggaaac ttattttgcg
     3541 atgaagtttt ttatcgataa ctggcgttgg agcggggtgc cgttttatat ccggtcggga
     3601 aagcgtttac ctacaagagt ttcagaaatt gtaatccatt tcaaaccgtc gcccacacaa
     3661 ctttttaaat cggttgatgg agctattgaa gcaggcaacc agctgatcct gcgtattcag
     3721 cccgatgagg gaattttatt aaaaacaggt atgaaggtcc cgggaaacgg ttacgaggtg
     3781 aaatcggtaa atgtggattt tcattattcc gacctgaaag accattatat ccctgaagca
     3841 tacgaacggt tgatcctgga ttgcatgacg ggtgacaaca ccctgtatat gtcgggggaa
     3901 ggggccgaat tgacgtggag atttgtgcaa cctattctcg attattggga aagtgatccg
     3961 gatgcacctt tgtacggtta tccttccggg tcttggggtc cggatagcgc ggataagctg
     4021 atcgaaggtg cgcagacctg gcgctatccg tgtaaaaacc tggcggatga tggggtttat
     4081 tgtgaattgt aaatcgtaat tccacttcaa acgtcaaata tatgcaaaca aaaatattca
     4141 atacaaaaga agaactgaac cgtgcgttta cggattacct gaaggagata ctaaaagacc
     4201 gggaaagggt tacagtagca ttatcgggag gctccactcc gaaatcactg tttgattact
     4261 gggctcaacg ccatgcaacc gatatcgact ggaaaagtgt tttcctgttt tggggagacg
     4321 aacgttgcgt gccgcccacc gatgagcaaa gcaattacaa gatgacccgg gatcatttac
     4381 tcgatcatgt ggccatcccg gaaagaaata tttttcgtat caagggggaa aatgaccctg
     4441 ctgccgaagc acaaagatat tctaccttac tgcaaatgga agtagaaacg gtggataccg
     4501 ttccttcgtt tgacctggtt attctggggt taggcgatga tggacacacg gcctccattt
     4561 ttccgcacga aatcgactta tgggaaagtg cagatttatg tgtggtggga acacatcccg
     4621 acagcggaca aaaacggata agtttgtcgg gaaaagtaat taatcatgct cagcgagtgg
     4681 cgtttttagt tacgggagat ggcaaagctg aaaaagtgaa acagatcatt gaatcgggaa
     4741 aagaagtagc cgcagcttat ccggctgcca aggtaaaacc cctgaccaac aacctggtat
     4801 ggtttttgga caaagaagcg gcaaggttgt tgggaaagta acgttattac accgttttta
     4861 ctcggtagtg gcagcttacc tttcctttct ccatgtttga taacttgctt tttatcattc
     4921 gtttgcgcaa cggggaaata cggtcggtaa acatgattcc gtcgagatga tcgtattcgt
     4981 gctggataac acgagccata taaccggtgt attcttcatc gtgcggctgc agatcttcat
     5041 cgagatattg aatgcggatt ctttcttcac gcggcacttt ttcatggata cccggaatgc
     5101 tcaggcaccc ttcttccacc cattccaact caccggtttt ttcggttatc tctgcattga
     5161 taaacacctt cttgaaactt ttgaattcag gatgttcatt atctgccagc ggctccaggt
     5221 caacgacaaa aatacggtct tccaagccta cctggggggc ggcaagccca acgccgtcgg
     5281 cactgtacat tgtttcatac atattgtcaa tcaactcctt caggttggga tacgttgccg
     5341 catcaatctc tttggctgtt tttcttaaaa gggggtgtcc gtacacgtat atgggtaaaa
     5401 tcattgtttg agtttatgag ttattgcctt ctttcttttt tataaatcaa gctttccata
     5461 tatccctgca ggattatggt ggcactgatt tcatcgatca aagctttgtt tcttctgtct
     5521 tttttcttta aaccgccgtc aatcattgtt tgttgagcca ttttcgatgt aaaccgttca
     5581 tcaaagcaga caacatccag atgagggtat aattttctga gctgatcggc aaactgctct
     5641 attttcggca ggttttccga cgcttcgtag ttcatttgcc ggggtaatcc aataattact
     5701 ttttcaactt tctctttttc aaaataagac cgcagaaagt cgaaaatacc ggcggtatcc
     5761 accgttgtca gtccgttggc tatgagttgt aacgggtcac tgaccgcaat acctgttctt
     5821 tttttcccgt aatcgatagc gaggagcctg ctcatggcaa catttcaaaa tttttgcggc
     5881 gcaattcaaa atcccgaccc aggtatttct cccttacgac cgggttctcc gccagttctt
     5941 ccgccgtccc ctgaaacaac accctgccct cgaaaagcag gtaagcccgg tcggttatgc
     6001 tcagcgtttc atcgacgttg tggtcggtga tcaggatgcc gatgtttctg tatttaagtt
     6061 gtgccacaat cgactgaatg tcttgaacgg caattggatc gacccctgca aaaggctcgt
     6121 cgagcataat gaacttggga tcaatagcca ggcaccgggc aatttcagaa cgacggcgtt
     6181 ctcctcccga caaacggtcg cccaggtttt tccgtacttt ttccaatccg aattcggtaa
     6241 tcaactcttc cagttttttc tttctttctt caggggtttt gttggtaaat tccaacaccg
     6301 acttgatgtt gtcttccacc gtcattttcc tgaaaataga ggcttcctgg gccaagtaac
     6361 ctatcccgta ctgtgcccgt ttgtaaaccg gatatttggt aatgttctga tcacccaggt
     6421 atatgcttcc ttcgttagga acaatcagtc ccacagtcat gtaaaacgtg gtggtttttc
     6481 cggccccgtt aggacccagc aagccgacaa tttcgccttg tttcaaatgg atggaaacgt
     6541 ggcttactac tgtcctttta ccgtattttt ttaccaggtt ttcggtacgt agcactaact
     6601 gcccgtcagg tacttcggct tgtctcgtct cgtcgttcat tacttatcgt aaatttatgc
     6661 aaagataata aatcatttac gatttggaat ttcggatggg ggatttatgc cttttttaca
     6721 tctatagcct attcgttgtc cgtgataatt atttccgaag cttccatctc tccgcttacg
     6781 ggcggattta gtttggccag gcgtacttcg agggagttaa ttcggggaaa tgatcttttt
     6841 atttccgaga atattcgtcc agcaacatgt tccagcaatt tactgggcac agccatttct
     6901 gctttcacca gatcataaac cgccgcataa ttgaccgtat cagccacgtt atcggtctca
     6961 aaagacctgg aaacctcggc agtgaacgtt acgttaacca caaaattatt cccgataatt
     7021 ttctcttgcg ggagtacgcc atggtaggcg taaaaccgca tgttttttag gttgaaggag
     7081 gtttgcataa atgattcatc ttgttaatag cgccaaatgt aagaacaatt tgttaaattt
     7141 gtggtttata aaatgaaaaa aatacccaac atgagtacat caaccatttc tgaacgatta
     7201 atcgccataa gaaattttat ggaagcagcc aagctggatg catttataat tcccagtacc
     7261 gatgcgcatt tgagcgagta cccgccgaaa aggtgggagt cacgcaagtg gataagcgga
     7321 tttaccggtt ctgccggaac agctgttgtg accaagaaaa gagccggtgt ctggacagat
     7381 tcgaggtatt ttcttcaggc agctgacgag ttggagggga cgggttttga cttgtttaaa
     7441 atgggacaac ccggcacacc tgaaatgacc gcatggatcg ttgaacaggt aggtaagggt
     7501 ggaacagtag gaatcgatgg gcttgtttat gccgcatccg atgccaaagc attgagatcc
     7561 gcgttggaag ctaaagatat acggctggag accacactcg acccgttcgc tgaagtgtgg
     7621 aaagaccgtc cggaaattcc cgggaacaag gtttttcttt tgtctgaaga gatcaccgga
     7681 gaatccacta aaagcaagat cgagagaatc catgacgaac tcgacagatt gggtgccgac
     7741 gggttaattg ctgttaccct ggatgccgta gcctggattt ttaatatgcg cggcagcgat
     7801 gttgatttta atccggtggc tgttgcctat ggctacgtat caaaaaaaga aagcgtactt
     7861 tttgtagatg aagaaaaact ggacgacctt accaaaaata ctctcttgaa acaaggagtg
     7921 aaaattgacg attacgaccg ggttttcagc tatgtcgcca atttacccga aaacactgcc
     7981 gtttgcctta ctggccataa aatcaattac aagttgtacc agaccctctc ggca
//
LOCUS       DAOSDR010000097         8031 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrimonas sp. isolate AMR_MDS_1826
            NODE_3323_length_8031_cov_3.207748, whole genome shotgun sequence.
ACCESSION   DAOSDR010000097 DAOSDR010000000
VERSION     DAOSDR010000097.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863355
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrimonas sp. (wastewater metagenome)
  ORGANISM  Petrimonas sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Dysgonomonadaceae; Petrimonas.
REFERENCE   1  (bases 1 to 8031)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8031)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:10:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,720
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 35
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8031
                     /organism="Petrimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3323_length_8031_cov_3.207748"
                     /isolate="AMR_MDS_1826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2023866"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..607)
                     /locus_tag="PLI30_05300"
     CDS             complement(<1..607)
                     /locus_tag="PLI30_05300"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding protein"
                     /protein_id="HOI79006.1"
                     /translation="MKERIGVYICHCGGNISDYVDVEKVRQAVENDKGVAFAKTTVFA
                     CSDSNQNDMVEDIKKHNLDGVIVASCSPKLHLVTFRNVTERGDLNKYTYVHTNIREQA
                     SWAHSDDKAGATEKTIHLVKAAIAKSRYAEPLYPHQFEAEKTIAVIGAGISGMKAAAT
                     LSDKNTHVILIEKEDRVGGHTATWGSLAMSDEKGCELSQRIH"
     gene            complement(604..1911)
                     /locus_tag="PLI30_05305"
     CDS             complement(604..1911)
                     /locus_tag="PLI30_05305"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoB--CoM heterodisulfide reductase iron-sulfur
                     subunit A family protein"
                     /protein_id="HOI79007.1"
                     /translation="MNEERKMLHYDALVVGGGIAGGEAALNLANTGYKVLLVEKDRSL
                     GGKMILLSKVFPTLDCSACITTPKVSEIARHKNITIFTESEVTAIVKKSENDFTAQVT
                     KKPRYVRVEDCTACQLCEKACPVSVRDEYQSGLIGRKAAFIPFSIANPKAAAIDIENC
                     TLCGACEKVCPTHCIDFTMETEYYELHVRTVVLATGFNLFDPLQIPRYGYGQHKNVIT
                     SMQMEREIAPTRPYNTILRPGDGKVPDRIAYVLCTGSRDATVGNPICSQICCMYSIKQ
                     AQLLMGALPMADVTIYYINIRSFGKGFEEFYQQAKGMGVNFVKGKIGKITEKENGNLI
                     LRYEDINEGVVKEAEHDMVVLSVGVKSNPTVGKMFGRGELKLDPFHFVAQDDLLGSPS
                     KTSIDGVFVAGTASAPMDIPDSILSAGSASSEAISYLIQHQEP"
     gene            complement(1918..2313)
                     /locus_tag="PLI30_05310"
     CDS             complement(1918..2313)
                     /locus_tag="PLI30_05310"
                     /inference="COORDINATES: protein motif:HMM:NF014692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase iron-sulfur subunit"
                     /protein_id="HOI79008.1"
                     /translation="MKKNARILVFSTEKISDPAIDMAGLLKLHYPPTVYTITVPCSSG
                     IKPSWILHAYEKGFDGVFIAADGSDCVFGETCTEKTGNVVSVTHDKMKESGIDPARLR
                     MAAICSVCSESFVKQIRSFNDYLIKTLHA"
     gene            complement(2328..2579)
                     /locus_tag="PLI30_05315"
     CDS             complement(2328..2579)
                     /locus_tag="PLI30_05315"
                     /inference="COORDINATES: protein motif:HMM:NF013379.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HOI79009.1"
                     /translation="MTTEELRTLTVAKSVDARGTACPGPLLEAKKAIGTLHSNDVMEI
                     LSADEGTKVDIPKWCGKQGHEYLGAVEENGYFKVYMKKK"
     gene            complement(2629..3195)
                     /locus_tag="PLI30_05320"
     CDS             complement(2629..3195)
                     /locus_tag="PLI30_05320"
                     /inference="COORDINATES: protein motif:HMM:NF025066.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE/DsrF/DrsH-like family protein"
                     /protein_id="HOI79010.1"
                     /translation="MESINALTLEKLQAELESLEAKIGRLEVSRKDQLSIAIVSGDMD
                     KILAAMIISLAAAAMDTQVKLFFSFWALSALRDPRKKVRGKDFISKMFGMMLPNGRNK
                     LKLSNMNMCGMGPVMIKKLMKKQNVMSLDAMFTEAAELGVEITVCEMSMNLMGFKKEE
                     MIDYPHLRYAGATTFVADAGESSMQWFI"
     gene            3328..4005
                     /locus_tag="PLI30_05325"
     CDS             3328..4005
                     /locus_tag="PLI30_05325"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HOI79011.1"
                     /translation="MKNECIDCACVMKSSSTLKNCQLEMLENNHAVVKFRKGDSIIKQ
                     GVFSTNVIFLRKGLVKIHITGPYREQIVRLIKAPTYLGLPTTLGDKINQYSVTAVLDS
                     EVCFIDIEVFKRVLEENRDFSSYIIMELSKSELEAYRRCANRTQKQTRGKLADVLLDF
                     SDTIFNSDEFVLPVSQSDIGNWVDAGRESINRVLSEFATDGLIEMTGRNVKIRDKKLL
                     KMISQNG"
     gene            complement(4015..4629)
                     /locus_tag="PLI30_05330"
     CDS             complement(4015..4629)
                     /locus_tag="PLI30_05330"
                     /inference="COORDINATES: protein motif:HMM:NF024579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI79012.1"
                     /translation="MEPLNKKSGWFILLLLFSATVSAQVNSSQVFADSYRYESSGDYQ
                     NAIAELNVLNGYDYHKSLRLGWLYYLAKDYESSKKFYNQAIGLAPQAVEALLGICYPL
                     EAQKKTDELETVYKKILSLDKANSKVNYALGNIYYYRKDFIQAEKYFDLVQTMYPFDY
                     YSTLMSAWTKYFLNKKNDAQRLFNIVLIISPTDQSAKEGLNLLK"
     gene            complement(4617..5930)
                     /locus_tag="PLI30_05335"
     CDS             complement(4617..5930)
                     /locus_tag="PLI30_05335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI79013.1"
                     /translation="MYRLGVTLIIVMVVASVLAQDSLRRELDSLTYQQYLNKDYKNLI
                     ETGKKAREQHINFYYLNYRTAIAYYELKNYAKAAEFYRKTLAETPDDPILQESLYYSY
                     LLSGQKENAAIVARSLAPHTQVTIGYKPSSVDYILLSGGYMTNDNTPDIRSDFAGNDS
                     VNQYRDMIFTSLGLGFNLSSRSRFKLGYQVYNTNFEQYTTTGLAQTDKLTQHQLVGTL
                     EFFTESNVLWGVAGGYYFIELFDRTAIYTLGGGGKGFGPGTGRIFTTSGGKSVSAFSA
                     LVFLNKRFAYTLPEIAATYSNFGGGHQYQVKGSLTYYPLGNLNFYGTTAAAVIRNQDA
                     WTDTQLIFSQSLGVKLTKQLWLDGNISLGNHRNYVSERSFLVYDTYDPVKAIAGLSLS
                     CFFRDVMLSAGYQWQQREGYAYSAINYSAYKYNNHLLNIAFTWNL"
     gene            complement(5912..>8031)
                     /locus_tag="PLI30_05340"
     CDS             complement(5912..>8031)
                     /locus_tag="PLI30_05340"
                     /inference="COORDINATES: protein motif:HMM:NF015226.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015204 - urea transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0071918 - urea transmembrane transport
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="urea transporter"
                     /protein_id="HOI79014.1"
                     /translation="SYAQIFFSQDRWYAVILLTVSMLDYRLGLGGLTAVVLTNLMAHL
                     LGFSKEKIRTGLYGFNAVFIGISMVYKFHVNPALFVLFFFSVILGFMLTIWFETLLSK
                     YKVPILTLPFVFTLFVVDLSFKTFTHIQPILPFDRFTIVLAEQMKVPWYNAVHFFDNT
                     EIPQMLYFYLKTMASIFFTDSILVGVIVVIALLFHSRIKSTVAFLGFFFAFVTSKMMG
                     VDIQQLTQNLAGVNYIFWGMAIGSFFIIPNAYSYLLVVGLTPVLFLFYTGIENLILNM
                     GLSSYTLSFSLLSILLLFILRQRSLNRFFVFPYIQYYNPEKTVYKNVNYMQRFGQETL
                     FKMQLPFLDKWTVSQGYDGAITHLGDWGKALDFVIMDEEGSTCFGRCAQKEDFYCYNK
                     PVLAPADGYVYTISNIAGDNEINQVDTRKNWGNTIIINHLNGLYTQISHLKKDSFKVR
                     TGDFVTKGTVVAACGNSGRSPEPHLHFQVQLTPEIGAVTYPYPIGYFFEKEKEKQVLR
                     IGEVPQENSTVWNVVASGLLLDAFEAKPGKLLRVKYNGEDFMWPVATDAYNKTYIHCR
                     KTKSTAYLENDGTMFYFTDFEGKKSSPLYLFYRSCFKLLLSCEKEIPVKDFVPLTKEH
                     PTGTRWIQDLLAPFVIFTRIKYRSELIEVDNMHFPEKVVYLTQTNTVSFHFKNRRKET
                     SVTVLKNSIEIHFQNEKLCIDWA"
BASE COUNT         2176 a         1880 c         1714 g         2261 t
ORIGIN      
        1 aatggatgcg ttgggagagt tcgcaccctt tttcatcgga catggccaag gagccccacg
       61 tagcggtgtg tccgcctacc cggtcttcct tttcaatgag aatcacatgc gtgtttttat
      121 cggaaagcgt ggcggcggcc ttcataccgg aaattcccgc tccgataacg gcaattgttt
      181 tctccgcttc aaactggtgg ggatacaagg gctccgcata acgcgatttg gctatggccg
      241 cctttaccag gtggatggtt ttttcggttg ccccggcttt atcgtcggaa tgtgcccagg
      301 atgcctgctc acggatattg gtgtggacgt aagtgtattt attcaaatca ccgcgttccg
      361 taacgtttct aaacgtcacc aaatgtaact tcggcgagca agaagctaca atgaccccgt
      421 ccaggttatg ctttttgatg tcctcaacca tgtcgttctg attggaatcg gaacaagcga
      481 acacggttgt tttggcaaaa gccactcctt tgtcgttctc caccgcctga cgcacttttt
      541 ccacatcaac gtaatcggag atgtttcctc cgcagtggca aatgtatacg cctattctct
      601 ctttcatggt tcctggtgtt gaattaaata actgatagcc tcggacgatg ccgagcctgc
      661 cgaaagaatg gaatcgggaa tatccatggg tgcggaagct gttccggcaa caaaaacacc
      721 gtcaatactg gttttggaag gacttcccaa taaatcgtcc tgcgctacaa aatggaacgg
      781 gtctaatttc agttcgccac gaccgaacat ttttccgacc gtaggattgg acttcacccc
      841 gacagaaagc accaccatat cgtgttcggc ctccttcacc accccctcgt tgatgtcctc
      901 ataacgaagg ataaggttgc cgttttcttt ttcggttatt ttcccgatct tgcctttcac
      961 aaagtttaca cccataccct tggcctgctg gtaaaactct tcaaatcctt ttccgaatga
     1021 gcggatattg atgtagtaaa tcgtcacgtc tgccatcggc aacgcaccca tcaacaactg
     1081 ggcctgcttg atggaataca tgcaacagat ctgcgaacag atgggatttc caacggtagc
     1141 atcgcgcgaa ccggtacaga gcacgtaagc aatcctatcg gggacttttc cgtcaccagg
     1201 acgtaaaatc gtattgtacg gacgtgtagg cgcaatctcg cgctccattt gcatggacgt
     1261 aatcacgttt ttgtgttgcc cgtagccgta tcgcggtatc tgaagcggat cgaacagatt
     1321 gaagccggtg gccagaacaa ccgttctgac atgcagttca taatattcgg tctccatcgt
     1381 gaaatcgatg caatgcgtag gacaaacttt ctcacatgca ccgcagagcg tacaattctc
     1441 aatatcgatt gctgcagctt tggggttggc gatgctaaaa ggaataaacg ccgcctttcg
     1501 gccaatcaac cccgattgat actcatcgcg cacactcacc ggacaggctt tttcgcacaa
     1561 ctggcaagcc gtgcaatcct ccacacgcac atagcgcggt tttttcgtta cctgagccgt
     1621 aaaatcgttt tctgattttt tgacaatagc agtcacttca ctctccgtga aaatggtaat
     1681 gtttttatgc cgggcaattt ccgacacttt gggtgtagtg atgcaggccg aacagtccag
     1741 tgtgggaaaa accttgctca ggaggatcat ttttcctcct agcgaacggt ctttctccac
     1801 aagcaacacc ttataaccgg tgttggccag gttaagcgcc gcttctcctc cggcaatccc
     1861 gcctcccaca accaacgcgt cgtaatggag catttttctt tcttcgttca tcgaaatcta
     1921 tgcgtgtaat gttttaatca gatagtcgtt gaagctgcgt atttgcttca cgaaagactc
     1981 gctgcatacc gaacagatgg ctgccatgcg caagcgtgcc ggatcgatgc ccgattcttt
     2041 cattttgtcg tgtgtgaccg aaaccacgtt tccggttttc tcggtacagg tctctccaaa
     2101 gacacaatcg ctcccatcgg cagcaataaa cacgccgtcg aaaccttttt cataggcatg
     2161 caagatccag ctcggtttta ttccgctcga acaaggaacg gtaatggtat aaaccgttgg
     2221 agggtaatgc aacttgagca atcccgccat atcaatggcg ggatcggata ttttctccgt
     2281 agaaaaaacc aatatccggg cgttcttctt catttcgaag caaaagttta ttttttcttc
     2341 atgtatactt tgaagtatcc gttttcttcc accgcaccca ggtactcatg accttgctta
     2401 ccgcaccatt tcggaatatc caccttggtt ccctcgtctg ccgaaagaat ttccatcaca
     2461 tcgtttgaat ggagggtacc tatggccttt ttagcttcca gtaacgggcc tggacaagct
     2521 gtaccccgtg cgtctaccga ttttgctact gtcaatgttc ttaattcttc tgttgtcata
     2581 gttgtaaatg tttactaatt atatatatgt gtcgttttta ttcttcgctt aaatgaacca
     2641 ttgcatactg ctttcaccgg catcggcaac gaatgtggta gcaccagcat aacgaagatg
     2701 aggataatcg atcatctcct ctttcttgaa acccatcagg ttcatcgaca tttcacaaac
     2761 cgtgatttcc actcccagtt cggcagcttc cgtaaacatc gcatcaagcg acatcacgtt
     2821 ttgcttcttc atcagttttt taatcatcac cggccccata ccgcacatgt tcatattcga
     2881 caacttcagt ttgttgcgtc cattgggaag catcattcca aacattttcg agataaaatc
     2941 ttttcctctt acctttttcc ggggatcacg caatgcggac aacgcccaaa aggagaagaa
     3001 aagtttcact tgtgtatcca tggctgctgc agccagggaa ataatcatgg ctgccagaat
     3061 cttatccata tcccccgata cgatggcaat agacaactgg tctttacggg aaacttctag
     3121 cctgccaatt tttgcctcca ggctttccag ttccgcctgg agtttctcca gggtcagggc
     3181 attaatactt tccatttaaa aagatgtttt tgagtgaatt aaccggacgc aaattaaaga
     3241 tatatttctt taattatttg ttttttcggg gtgaacattc ttacaatatt tagtgataaa
     3301 tgtcacattt ttatctattt ttgctgtatg aaaaacgaat gcatcgattg tgcctgtgtg
     3361 atgaaatcat cgagcacact aaaaaattgt cagcttgaga tgttggagaa caaccacgcg
     3421 gtggtaaaat tccgcaaagg agattccatc attaaacagg gcgttttttc cacgaacgtt
     3481 atctttcttc ggaaaggact ggtaaaaatt catatcacag ggccctatcg cgaacaaatt
     3541 gtccgcctta taaaagcacc aacctatttg ggtttaccca ctacactggg tgacaagata
     3601 aatcagtaca gcgttacggc agtattggac tcggaggttt gttttatcga cattgaagtt
     3661 tttaaacgtg tgctggaaga aaacagggat ttctcttcct atatcatcat ggagttgtcc
     3721 aaaagcgagc tggaggctta tcgccgatgc gccaaccgca cgcaaaaaca aacacgcggc
     3781 aagcttgctg acgtattgct cgacttttcc gatactattt ttaattcgga tgagtttgtc
     3841 ctgccggttt cacaatcgga tatcggaaac tgggtggacg caggcagaga aagcatcaac
     3901 cgtgtccttt cggaattcgc taccgatggg cttattgaga tgacgggaag aaacgtgaaa
     3961 atacgcgaca agaaactcct gaaaatgatt agccaaaacg gctagaaaaa atggctattt
     4021 tagaagattc aacccctctt ttgctgactg gtcagttgga gagatgataa gaacaatatt
     4081 gaagagtcgc tgggcatcgt tttttttgtt gagaaaatat tttgtccagg cgctcatcag
     4141 tgttgaatag taatcgaaag gatacatggt ttgcaccagg tcaaaatatt tttcagcctg
     4201 tataaaatct ttccggtaat aatagatatt tcccagcgca taattcactt ttgagtttgc
     4261 cttatccagc gacaggattt ttttatagac cgtttccagt tcatccgttt tcttttgtgc
     4321 ttctagcgga taacaaattc ccaacaaggc ttcaacagcc tgcggtgcta aaccgatagc
     4381 ctgattataa aactttttgc tgctctcgta atccttagcc aggtaataca gccaaccgag
     4441 gcgcaatgat ttgtggtaat cgtatccgtt caggacattc aattcggcta tggcattctg
     4501 ataatcgccc gacgattcgt atcgataact gtctgcaaaa acctgagatg aattcacttg
     4561 tgctgaaact gttgctgaga acaacaacaa aaggataaac catccgcttt ttttatttaa
     4621 aggttccatg tgaatgcaat atttagaagg tgattgttgt atttataggc agagtagttg
     4681 atcgcagaat aagcatatcc ctctctttgt tgccactggt atcctgcaga cagcatgaca
     4741 tctcggaaaa agcaggaaag agaaagcccg gcaatggcct tcacgggatc gtacgtgtca
     4801 tacaccagaa aagagcgttc tgaaacgtaa ttccggtgat ttcccaggct tatgtttccg
     4861 tcgagccaca actgtttggt taactttact ccgaggctct gagagaatat aagttgagtg
     4921 tccgtccagg cgtcttggtt gcggatgact gctgcagcag ttgttccgta aaaattgaga
     4981 ttacctaacg gataataagt aagcgatccc tttacctgat actggtgccc gcccccaaaa
     5041 ttggaatagg ttgcagcaat ctcgggtaaa gtgtacgcaa agcgtttgtt cagaaatact
     5101 agtgcagaaa atgccgaaac ggattttccg cctgaagttg tgaagattct cccggttccc
     5161 ggtccgaatc cctttccacc gcctccgaga gtataaatgg ctgtcctatc aaacaattca
     5221 atgaagtaat acccccctgc aactccccac aaaacattgc tttccgtaaa gaattccaat
     5281 gtcccaacca attgatgctg cgtgagcttg tcggtctgag caagcccggt ggttgtatac
     5341 tgttcgaaat tcgtgttata cacttggtag ccgagtttaa atctggaacg ggaagaaaga
     5401 ttaaagccca atcccaagct cgtgaaaatc atatctctat attgattgac ggaatcgttt
     5461 cccgcaaaat cagagcgtat atccggtgta ttgtcgtttg tcatatatcc gcccgataat
     5521 aaaatgtaat ctacggacga aggcttgtat cctatggtta cctgcgtgtg cggcgcaagc
     5581 gatctggcaa cgatagctgc gttttctttc tgtccgctta acagatagga gtagtaaaga
     5641 ctttcctgca aaatcgggtc atctggggtt tcggcgagcg tctttctgta gaattctgct
     5701 gccttcgcat aatttttcag ttcgtagtag gcaatggcgg tccggtaatt gaggtagtaa
     5761 aaattaatat gttgctcacg ggccttctta cccgtttcta tcaggttttt gtaatccttg
     5821 ttgaggtatt gctgatatgt gaggctgtct aattctctgc gcaggctgtc ctgagcaaga
     5881 accgaagcca caaccatgac tataattaac gttacgccca atcgatacat aatttttcgt
     5941 tttggaaatg gatttcaatg ctgtttttta aaaccgtcac cgatgtttct ttccggcggt
     6001 ttttaaaatg aaacgatacc gtatttgttt gtgtcaggta aacaactttt tcgggaaaat
     6061 gcatgttgtc cacctctatc aattccgacc ggtacttgat tcgagtaaaa attacaaacg
     6121 gagccaacag gtcctggatc caccttgttc cggtggggtg ctctttcgtc aggggaacga
     6181 agtcctttac aggaatttct ttttcacagg aaagcaggag tttaaagcaa ctccgataaa
     6241 aaagatacaa cggcgatgat tttttccctt caaaatcggt aaaatagaac attgtgccgt
     6301 cgttctccaa gtaagccgtc gattttgttt tccggcaatg tatatacgtt ttgttataag
     6361 catccgtagc taccggccac ataaaatctt cgccgttgta ttttacacgt agcagttttc
     6421 ccggtttagc ctcaaatgcg tcaagcaaca gacccgatgc cactacattc caaacggttg
     6481 aattttcctg cggaacctca ccgatacgga gcacctgttt ttccttttct ttttcgaaaa
     6541 aatacccgat tggatacgga taggttaccg caccgatttc cggtgtcagc tgcacttgaa
     6601 aatgaagatg aggctccgga gagcgtcccg aattgccaca tgcggcgacg acggttcctt
     6661 tcgtcacaaa atcgccggta cgaaccttga aactgtcttt cttcaggtga cttatctgtg
     6721 tatataatcc gtttagatga ttaataataa ttgtattgcc ccaatttttc cgtgtgtcaa
     6781 cctggtttat ctcattgtct ccggcaatgt tgctgattgt atagacataa ccgtcagcag
     6841 gagcaagaac cggtttgttg taacagtaga aatcttcttt ctgcgcacac ctgccgaagc
     6901 aagtactccc ctcttcatcc ataatcacaa agtcaagcgc ttttccccag tcgcccaaat
     6961 gcgtgatagc tccatcgtat ccctgactga ccgtccactt gtctaaaaat ggaagttgca
     7021 tcttaaacaa ggtttcctgt ccaaaacgtt gcatgtagtt tacattcttg tacaccgtct
     7081 tttcaggatt ataatattgt atatagggga aaacaaaaaa tcggttcagg cttcgctgcc
     7141 ttaaaatgaa caacagcaat atcgacaaaa gactgaacga caaggtatat gaagacaacc
     7201 ccatattcaa aataaggttt tcgatacctg tataaaaaag gaaaagcacc ggggtaagcc
     7261 ctacaaccaa caagtagctg tacgcgttgg ggataataaa gaaactgccg atagccattc
     7321 cccagaaaat ataattcacc ccggccaggt tttgggtcag ctgctgaata tccactccca
     7381 tcattttgct tgtcacaaaa gcgaagaaaa atcccagaaa agccaccgtc gatttaattc
     7441 tcgaatgaaa cagcaacgca attacaacaa tcacccccac taaaatgctg tcggtaaaga
     7501 aaattgaggc catcgtcttt aagtaaaaat agagcatctg cgggatttcg gtattatcga
     7561 aaaagtgcac ggcattatac cagggaactt tcatctgttc tgccagaaca attgtaaaac
     7621 ggtcgaaagg aagaatgggc tggatatggg taaatgtttt aaatgaaagg tcaactacaa
     7681 acaacgtgaa tacaaacgga agagtgagga tgggaacttt gtatttggat aacagggttt
     7741 caaaccagat cgtcagcata aatcccaaaa taacagaaaa aaagaaaagc acaaacagcg
     7801 ccgggttcac gtgaaactta tataccatac tgatcccgat aaaaacagcg ttgaaaccat
     7861 atagcccggt tctgattttt tctttcgaga aaccgagcag gtgcgccatc aggttggtca
     7921 agaccaccgc agtaagccca cccaatccca accggtaatc gagcatactg accgtcagta
     7981 aaatgacggc gtaccatcta tcctgagaaa agaatatctg ggcatagctg t
//
LOCUS       DAOSDR010000098         8024 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrimonas sp. isolate AMR_MDS_1826
            NODE_3332_length_8024_cov_4.320492, whole genome shotgun sequence.
ACCESSION   DAOSDR010000098 DAOSDR010000000
VERSION     DAOSDR010000098.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863355
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrimonas sp. (wastewater metagenome)
  ORGANISM  Petrimonas sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Dysgonomonadaceae; Petrimonas.
REFERENCE   1  (bases 1 to 8024)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8024)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:10:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,720
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 35
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8024
                     /organism="Petrimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3332_length_8024_cov_4.320492"
                     /isolate="AMR_MDS_1826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2023866"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(139..453)
                     /gene="trxA"
                     /locus_tag="PLI30_05345"
     CDS             complement(139..453)
                     /gene="trxA"
                     /locus_tag="PLI30_05345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008998931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HOI79015.1"
                     /translation="MALQITDATLNEVLATDKLVVIDFWAEWCGPCKMVGPIIDQLSE
                     EYKDRVVVGKIDVDNNDEATSKYGIRNIPTVLFIKNGEVVDKVVGAGAKTLFSEKIEK
                     NL"
     gene            complement(469..4194)
                     /gene="dnaE"
                     /locus_tag="PLI30_05350"
     CDS             complement(469..4194)
                     /gene="dnaE"
                     /locus_tag="PLI30_05350"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006842821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HOI79016.1"
                     /translation="MQPFVHLHVHSQYSLLDGQASINRIVDKAMNDGMAGIALTDHGA
                     MYGIKEFVNYINSVKSKTKNEIKQLSKELESNPASQKLKEEIAAKTAKLNFKPIIGCE
                     CYCARRNRFSQTEKIDGSGWHLVVLAKNLTGYKNLIKMVSKSWTEGFYYRPRIDKELL
                     EQYHEGLIVSTACLGGEIPRKIDDGNVDGAEEAVEWFKNIFGDDFYLEIQRHKTDRPD
                     ADQTTFPKQQRVNEEIIKIGKKYGVKVIATNDAHFVNEEDAEAHDRLICLSMGKDFDD
                     PDRMRYTKQEWIKTTAEMNRIFSDVPEALANTMEIADKVEIYSIDNPALMPFYPIDSS
                     FGTEEEYKTRYPEAALIEKFGETNFKRLGGYDKAIRIQLESDYLRHLTKIGARKKYGE
                     ELSEEVTSRLKFEIDTITNMGFPGYFLIVQDFINAARQMDVAVGPGRGSAAGSAVAYC
                     LGITDIDPLKYDLLFERFLNPDRISMPDIDIDFDDEGRDKVLQWVTEKYGFEKVARII
                     TYGTMATKSSIRDVARVHKLPLAEADRLAKLVPDRIPNVKKINLREAIEHVPELKEAA
                     NNPDPLVRDTLKYAEMLEGNVRSTGVHACGVIIGQTDISDIVPISTADDKETKEKLLV
                     TQYEGSVIEETGLIKMDFLGLKTLTIIKDALINIETTHGIKIDINTIPLDDPKTYELY
                     SNGQTTGTFQFESTGMQKYLKELHPSKFEDLIAMNALYRPGPMDYIPSFIARKQGKEK
                     IVYDIPVMEKFLDETYGITVYQEQVMLLSRLLANFTRGQSDELRKAMGKKLADKMAAL
                     KEKFISGGKSNGYKEKDLQKIWTDWEKFAQYAFNKSHSTCYSWVAYQTAWLKANYPSE
                     YMASVLSNNLNNITEITKFMDECKAMGINVLSPDINESVLKFSVNKSGHIRFGLAAIK
                     SVGHGAVEGILEERTNNGPFKDVFDFVERVNLSSCNKKNIESLALAGAFDSLPGVKRE
                     HFLSVNSKGEDFLETLIRYGNKFQQDKYLSMNSLFGDDTSFMISRPELPKVEKLSDLV
                     RLNKERELIGIYLSAHPLDDYEFILNYVCTSSCALLQDLNPLNGKDVSFGGLVTTVRE
                     GQTKRGSPYTIMAIEDYTGSCELAFFGDDSVNFGRFARLGLSIYVTAKIQPKRFRPEE
                     LEIKVISINLLSEVKDKLVTKITLQLPITELDDTLVSELSALMKNNSGHSLLYFNIIG
                     EAYNMNVQLFSRPSKIQVNKHLIDNLKNNLNIDFKIN"
     gene            complement(4361..5905)
                     /locus_tag="PLI30_05355"
     CDS             complement(4361..5905)
                     /locus_tag="PLI30_05355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008643811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HOI79017.1"
                     /translation="MFKNHPKGLIGAALSNMGERFGFYIMMAILSLFLLSKFGLDKTQ
                     ATVIYSIFYALIYLLALAGGFIADKTKKYKGTILWGLILMTIGYFLIAIPTPTPVPNF
                     ALFLAMTCFGLFVIAFGNGLFKGNLQAVVGQMYDDPKYSDKRDTGFQIFYMFINIGAL
                     FAPLIAVGIRNWWVQHNGFEYNSDLPALSHQFLRGTITPEATQRFTELSASVSKTPPA
                     DLTAFANEYLNVFNTGFHYAFGVAIFAMLISLTVFIINKRKFPDPALKAARSKESTDA
                     QPNENQMDSKEVRQRLYALFSVFAVVIFFWFSFHQNGVTLTFFANEYTDLSTINIDLG
                     FTSIKGAEIFQFFNPFFVVFLTPVIIGLFGWLRARGKEPSTPKKIAIGMGIAATAFLV
                     MSIGSQGLPDTDTARAMGGLTDAQRVTPFLLIGTYFILTVAELFISPLGLSFVSKVAP
                     PQYQGIMQGAWLGATALGNQLLIFGTIFYESLPLWTTWLVFVGACLISMFTMLYMLKW
                     LERVAK"
     gene            6332..7177
                     /locus_tag="PLI30_05360"
     CDS             6332..7177
                     /locus_tag="PLI30_05360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005933644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HOI79018.1"
                     /translation="MKKVAIQGGLGAYHDIAARNYFGEEIEIVPCLTFKEIFLKAQKD
                     EHLIGIMAIENTIAGGLLLNHDLLKLNDMKIAGEHKLRISHTLSALPGQRLQDIREIV
                     SHPMALMQCSDFIESLPQVKVVEHEDTALAAREIMEGNTPGIAAICSKLAAEVYHLEV
                     IREGIETNKRNFTRFLVLAHEEMVGEIRKSSDIDKASLVFVLPHSEGSLSRVLSVLSF
                     YDINLTRIQSLPIIGREWEYQFYIDLTFSNFERYRQSIKAILPLVGNLKVLGEYVEDK
                     HIVAD"
     gene            7216..>8024
                     /locus_tag="PLI30_05365"
     CDS             7216..>8024
                     /locus_tag="PLI30_05365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005868247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HOI79019.1"
                     /translation="MDTVLNIEPAGHIKRISEYYFSVKLAEVAKMNAEGKNIISLGVG
                     APDKMPSQETIETLCDTACRPDVHAYQPYSGIPELRKAYAGWYKKFYNVDLSTDEVLP
                     LIGSKEGILHVSMTFLNFGDSVLVPNPGYPTYRSVSELLRANVVEYDLLEKNEWLPDF
                     DALEKLDLTRVKLMWVNYPNMPTGKNASLALFEKLVAFGKKHGIVICHDNPYSFILND
                     RPMSILSVQGAKDICIELNSLSKSHNMSGWRMGLLASNPEFVRWVLKAKSN"
BASE COUNT         2102 a         1780 c         1867 g         2275 t
ORIGIN      
        1 gaggtcggaa aacgaaagta ggaagaaaac tacttcgttt tcctcctact cgattgatat
       61 ctacacccta taaaaaaaaa tcacgaagca ccataaggga gctccgtgag tggatgtttt
      121 aaaatgggga gagtagcatc aaagattctt ctctattttt tcggagaaaa gagttttggc
      181 gccggcaccc actaccttat ccactacctc gccgtttttg atgaaaagaa cggtgggaat
      241 attgcggata ccgtattttg aagtggcttc atcgttgttg tcaacatcta tttttcccac
      301 cactacacgg tctttatatt cttcactcag ctgatcgatg atgggaccaa ccatcttgca
      361 gggtccgcac cattcagccc aaaaatcgat aaccactaac ttgtctgttg ctaacacttc
      421 gttaagtgtt gcatctgtaa tttgtaatgc catagttatt tttttgaatt aattaatttt
      481 gaagtcgatg ttcaaattgt tctttaagtt gtctatcagg tgtttgttta cctgaatttt
      541 agacggacgc gaaaaaagtt gtacattcat gttgtacgct tcgccgataa tgttgaaata
      601 taacaacgaa tggcccgaat tgtttttcat cagagccgat aattccgaaa ccaacgtgtc
      661 gtccagttcg gtaatcggga gctgaagtgt tattttggta accagcttgt ctttcacctc
      721 agaaagcagg ttgatggaga ttacctttat ttccagctct tccgggcgga atcgcttggg
      781 ttggattttg gccgtgacat aaatagaaag tccgaggcgc gcaaacctcc cgaaatttac
      841 actgtcatct ccgaaaaacg ccagctcaca gctgccagta taatcttcta tagccatgat
      901 cgtgtagggg ctgccgcgtt ttgtctgtcc ctcccttacg gtggtaacca ggccgccaaa
      961 gctgacatcc ttgccgttaa ggggattcag gtcctgtagc aatgcgcaac tggaagtgca
     1021 aacatagttc aatatgaatt cataatcatc cagcggatgt gccgacaaat aaattccgat
     1081 aagttcccgt tctttattca atcgtaccag gtcggataat ttctccactt tcggcagttc
     1141 ggggcggctg atcataaacg atgtatcgtc tccaaaaagt gaattcatgg atagatactt
     1201 gtcctgctga aacttgttcc cgtaccggat aagcgtttcc aaaaagtcct cccctttgct
     1261 gtttaccgat agaaaatgtt cccgttttac tccgggaagc gaatcgaaag ctccagccaa
     1321 tgccagtgat tcaatgtttt tcttgttaca cgaagataga ttcacccgct ccacaaaatc
     1381 aaacacatct ttaaacgggc cattgttcgt cctttcttcc agtatgcctt ccacggctcc
     1441 gtggccgaca ctttttatgg cagccagccc gaagcggata tggccggatt tgttcacaga
     1501 gaacttgagt accgattcgt tgatgtccgg cgaaagaaca ttgatgccca tggctttgca
     1561 ctcgtccata aacttggtga tttcagtgat gttattcagg ttgttagaca acaccgacgc
     1621 catatattcc gatgggtagt ttgctttcag ccaggcggtc tggtaggcaa cccacgaata
     1681 acaggtggaa tgcgatttat tgaaggcgta ttgagcaaat ttttcccagt ccgtccaaat
     1741 cttttgcagg tctttttctt tgtagccgtt ggatttccct ccggaaataa atttttcttt
     1801 caaggccgcc attttgtccg ccaatttctt gcccatggcc ttgcgcaact catccgattg
     1861 tccccgggtg aaattggcca gcagtcgcga caaaagcatc acctgctcct ggtaaaccgt
     1921 gattccatag gtctcgtcca gaaatttctc cattaccgga atatcgtata cgatcttctc
     1981 ttttccttgc ttgcgggcga taaaagaagg gatataatcc atcgggccgg gacggtacag
     2041 ggcattcatt gcaatcaggt cttcaaactt gctggggtga agctctttca ggtacttctg
     2101 catcccggtt gactcaaact ggaatgtacc ggtggtttgg ccgttactgt acagttcgta
     2161 tgtttttgga tcatccagcg gaatggtgtt gatgtctatc ttgatgccgt gtgttgtttc
     2221 gatgtttatg agcgcatcct ttatgatggt caaggtttta agtcccagaa agtccatctt
     2281 tatcaacccc gtctcttcga tgacggagcc ttcgtattgc gtcacaagca gcttttcctt
     2341 tgtttccttg tcgtcagcag tgcttatagg gacaatgtcg gatatatcgg tttgtccgat
     2401 aataacgcca caggcgtgta ctccggtact gcgaacgttc ccctcgagca tctcagcata
     2461 tttgagcgta tcccggacca gcgggtcggg gttgttggcg gcttctttga gttccggaac
     2521 gtgttcaatg gcttcccgaa gatttatttt ttttacgttg ggaatcctgt cgggaaccag
     2581 ttttgccagc cggtctgctt ctgccagagg cagtttatgc acgcgggcca catcacggat
     2641 agacgatttt gtagccatag tcccgtaggt gatgatgcgg gcgacctttt cgaatccgta
     2701 tttttcagta acccattgga gtactttgtc gcggccttcg tcgtcgaaat caatgtctat
     2761 atcaggcatg gagatacggt ccgggttgag gaaccgctcg aacagcagat cgtatttcag
     2821 cggatcgata tccgtgattc ccaggcagta tgccacagcc gatccggctg ccgatccacg
     2881 gcccggacca accgcaacgt ccatttggcg tgccgcgtta ataaagtcct ggacgatcag
     2941 gaaatacccg ggaaatccca tgttggtaat ggtatcgatt tcaaacttca accgtgaggt
     3001 aacctcttcg ctgagttctt ccccgtattt tttgcgtgcc ccgattttgg taaggtgcct
     3061 aagatagtcc gactcgagct ggatacggat tgctttgtcg tatcctccca accgcttaaa
     3121 attggtttca ccaaactttt cgatcaatgc tgcttccggg tacctggtct tatattcttc
     3181 ttcggttcca aacgaactgt cgatggggta aaaaggcatc aatgccggat tatcaatgga
     3241 ataaatttct accttgtcgg caatctccat cgtgttggcg agcgcttcag gcacatcact
     3301 aaaaattcga ttcatctcag ccgtggtttt tatccactcc tgtttggtat agcgcatccg
     3361 gtcggggtca tcgaaatctt tccccatgct caggcagata agccggtcgt gcgcttcggc
     3421 atcctcttcg tttacgaagt gagcgtcgtt ggtagcaatc accttcacac cgtatttctt
     3481 gccgattttg ataatctctt cgttgacacg ttgctgtttc ggaaaagtcg tttggtcggc
     3541 atcggggcgg tcggttttgt ggcgttgaat ttcgaggtaa aaatcgtcgc cgaaaatgtt
     3601 tttaaaccat tcaaccgctt cttctgctcc gtccacgttg ccatcgtcta ttttccgcgg
     3661 tatctcaccc cccaggcaag ccgtggagac gatgagtcct tcgtgatatt gctcaagcag
     3721 ttccttgtcg atgcgcggac ggtaataaaa cccttcggtc cagcttttag ataccatctt
     3781 gatgaggttt ttgtatccgg tcaggttttt ggcaagcaca acaagatgcc agccactgcc
     3841 gtcaattttt tccgtttgtg aaaaacggtt gcggcgcgca cagtaacatt cgcagccaat
     3901 aatgggcttg aaattgagtt tggcggtttt ggcagcaatc tcctctttta gtttttggct
     3961 ggcaggattg ctttccagtt ccttgctcaa ttgttttatt tcgttttttg ttttcgattt
     4021 gacactgttg atataattca cgaactcctt gatcccgtac atcgctccgt ggtcagtcag
     4081 tgcgattccg gccatcccgt cgttcatggc tttatcaaca atccggttga tactggcttg
     4141 tccgtcgagc aaggaatatt gggagtggac gtgtaaatgt acaaatggct gcataagaga
     4201 aaaataaatg gattttccgt tttaacagca acaaatttag ccaaaaaaac agccggcccg
     4261 agaagaaata gatagaagtt atcaacattg ggaacaaaca aaaaccggtg agcattgccc
     4321 accggttcag ttcaacgttt tcgaaaaagt tgatcctatt ctatttcgcc acccgttcga
     4381 gccatttcag catatacaac atggtgaaca tagagataag gcacgctccc acaaatacca
     4441 gccaggttgt ccacaacggt aagctttcgt agaaaatggt gccgaagatc agcagctggt
     4501 tcccgagagc cgtagctcca agccatgctc cttgcataat tccctgatat tggggtggag
     4561 ccacttttga cacaaaagat aatccgagag ggctgataaa cagctcagca acagttaaaa
     4621 taaagtaggt gccaataagc aaaaatggag tcacgcgttg cgcatcggtc agtccgccca
     4681 tagcccttgc cgtatccgtg tctggcagcc cttgtgaacc aatggacata accaggaagg
     4741 cagtggctgc aatccccatc ccgatagcta tcttctttgg agtggaaggc tcttttcccc
     4801 ttgctctaag ccagccaaag agtccgataa ttacaggagt aagaaatacc acaaagaaag
     4861 ggttgaagaa ctggaatatt tcagcacctt ttatacttgt aaatcccagg tcgatattga
     4921 ttgtgctcaa atccgtgtat tcattggcga agaaggtgag ggtcacgccg ttttggtgaa
     4981 acgagaacca gaagaagatt actacagcaa acaccgagaa cagggcatac aggcgctgac
     5041 gaacttcttt tgaatccatt tgattttcat tcggttgagc atcggtgctt tctttggatc
     5101 tcgcagcttt cagggccggg tcggggaatt ttcttttgtt tataatgaat actgtcagag
     5161 agatcagcat ggcgaaaatg gctacgccaa aagcataatg aaaaccggtg ttgaatacat
     5221 tcaggtattc gttggcaaaa gcggtgaggt cggccggtgg tgttttgctc acagatgcag
     5281 aaagttctgt gaaacgttgt gtggcctccg gcgttatggt tcctcttaga aactggtggc
     5341 taagggccgg aagatcggag ttgtattcaa agccattgtg ttgaacccac cagttacgga
     5401 tgcccacggc aataagcgga gcaaaaaggg caccgatatt gataaacatg tagaaaatct
     5461 gaaatccggt atctctctta tcggaatact tggggtcgtc gtacatttgt cctaccaccg
     5521 cttgtagatt gcccttaaac agtccgttgc caaaagcaat gacaaacagt ccgaagcagg
     5581 tcatggccag gaacaacgca aaattcggaa cgggagtagg tgttggaatg gcaatcagga
     5641 agtaacctat tgtcataagt atcaagcccc acaagatggt tcccttgtac ttttttgttt
     5701 tgtcggcaat gaagccgccg gcaagtgcaa gcaaatagat cagagcataa aaaatcgaat
     5761 aaatgacagt ggcctgtgtc ttgtccagtc cgaatttaga taaaaggaat aacgatagta
     5821 tcgccatcat gatgtagaaa ccaaaacgct cgcccatgtt ggaaagggcg gcgccaataa
     5881 gtcctttagg atgatttttg aacataaatt gtttttttag atggtttgga atttttttaa
     5941 tttgcaaata tacataaata aatgaaatat ttaacttggg caagttgtag attcagccac
     6001 taacgggaat aacatgcggt acttgaatgg aatataaaaa tatcgacaaa cggggtagtt
     6061 attcctgttt tgaaaaaaat gaacaaaata tttcttcctt ttgttgtatt atttaatttt
     6121 tgttgtaatt ttgcaaccga tattcgcaaa taacgaataa aatgaaacag aaccattttg
     6181 cctattcttt ctatttttac ttttacttta gtaagtagaa caggatttga tgtgcaaaat
     6241 gaagaaataa aaaataactt ttttgaatag aaaatcctgt cgatagcacg acgggatttt
     6301 tttattttaa tagacttagc tctcaaaaca gatgaaaaaa gttgccatac aaggcggatt
     6361 aggcgcctac cacgacattg ctgcccgcaa ttacttcggg gaggagatcg agattgtacc
     6421 ttgccttacg tttaaagaga tttttttaaa agctcaaaaa gatgaacatc taattggaat
     6481 tatggcgatc gagaacacga ttgccggggg gctgttgctc aatcacgacc tgcttaaact
     6541 caacgacatg aagattgcgg gggagcataa attgcgtatc tcccatacgt tatcggcgct
     6601 gcccggacaa cgtttgcaag acataaggga aattgtttcg cacccgatgg cactgatgca
     6661 atgttccgat tttatcgaaa gccttcccca ggtgaaggtc gttgagcacg aggatactgc
     6721 tttggctgcc cgggaaataa tggaaggaaa caccccgggg atagcggcca tttgcagcaa
     6781 gcttgccgcc gaggtatatc acctggaagt tataagggaa ggaatagaaa ccaacaaacg
     6841 taactttacc cggtttctgg tattggctca cgaagaaatg gtcggtgaaa tacggaagtc
     6901 atccgatata gacaaggctt cgcttgtttt cgttctgcca cacagtgaag ggagcctttc
     6961 ccgggtgttg tcggtgctct cgttctacga catcaacctt acgcggattc agtcgctgcc
     7021 gattatcggc cgggagtggg aatatcagtt ttacatcgac ctgacttttt ccaatttcga
     7081 acggtaccgt cagtctatca aggccatact gcctttggtg ggaaacctca aggtgctggg
     7141 tgagtatgtc gaagacaaac acattgttgc cgactgaata acaaccgaat aactatttaa
     7201 caactatcga acaaaatgga tacagtactt aacatagaac ctgccgggca cataaaacgc
     7261 atttcggaat attacttttc ggtgaagctt gccgaagttg ccaaaatgaa tgcggaaggc
     7321 aaaaacatca tcagcctggg ggtgggagca cccgataaaa tgccgtcgca ggaaacgatt
     7381 gaaactttgt gtgatacggc atgtcgcccc gatgtacatg cttatcagcc ctactccggg
     7441 ataccggaat tgagaaaggc ttatgccgga tggtataaga agttttacaa cgtcgacttg
     7501 tcgacggacg aagtattgcc gcttatcggc tcaaaagaag gaattttaca cgtttcgatg
     7561 acatttctta actttggtga ctcggtattg gttcccaatc cggggtaccc aacctacaga
     7621 tcggtttcgg aacttcttcg tgcgaacgtc gtcgagtacg atttgctgga gaaaaatgag
     7681 tggctgcccg attttgatgc cctggaaaag ctggacttga cccgggtaaa gctgatgtgg
     7741 gtaaattatc ccaatatgcc taccggtaaa aatgcatcct tggcgttgtt tgaaaaattg
     7801 gtggcttttg gtaaaaaaca cggtattgtc atttgtcatg ataatccata cagttttatc
     7861 ctgaatgatc gtccgatgag tatcttgtcg gtgcagggtg caaaagacat ttgcattgag
     7921 cttaactcgt tgagcaaatc acacaatatg tcgggttggc gtatggggct gctggcatcc
     7981 aatccagagt tcgtaagatg ggtattaaaa gccaagagca atat
//
LOCUS       DAOSDR010000099         8004 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrimonas sp. isolate AMR_MDS_1826
            NODE_3345_length_8004_cov_4.122657, whole genome shotgun sequence.
ACCESSION   DAOSDR010000099 DAOSDR010000000
VERSION     DAOSDR010000099.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863355
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrimonas sp. (wastewater metagenome)
  ORGANISM  Petrimonas sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Dysgonomonadaceae; Petrimonas.
REFERENCE   1  (bases 1 to 8004)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8004)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:10:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,720
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 35
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8004
                     /organism="Petrimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3345_length_8004_cov_4.122657"
                     /isolate="AMR_MDS_1826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2023866"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(474..550)
                     /locus_tag="PLI30_05370"
     tRNA            complement(474..550)
                     /locus_tag="PLI30_05370"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(514..516),aa:Met,seq:cat)
     gene            complement(654..1736)
                     /locus_tag="PLI30_05375"
     CDS             complement(654..1736)
                     /locus_tag="PLI30_05375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2891 domain-containing protein"
                     /protein_id="HOI79020.1"
                     /translation="MKPLSSILFINLFLSMVLSPLFSQKNPDLTDETALKLSALPLGC
                     IDREYPNKPGHTYGSADEVGLSPKALHPVFYGCFDWHSSVHGHWMLIRLLKTKPGIAS
                     KNEIITLLNNSFDKAAVEVEKEYFLKYETGKGFERTYGWAWLLKLDEELASWDNPAAR
                     QWHENLQPLTGQIVELWREYLPKQTYPNRTGVHPNSAFALGFAIDWARENGNKEFEKE
                     LIGKSLDFYGKDTETPAHLEPDGADFFSPSLEIADLMRRILSRDDFEKWLVAFYTQKG
                     IDNISQIPVVSDLDDYQTVHLVGLSFSRAWCMKGIAKSLSEGHPLKLHFEETAQKLLD
                     HALPLVFDGNYGGEHWLASFALMALE"
     gene            1838..4270
                     /locus_tag="PLI30_05380"
     CDS             1838..4270
                     /locus_tag="PLI30_05380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005862081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HOI79021.1"
                     /translation="MIYPENFEQKIEFFKIRQLLEQHCLSPLGKEKVEEMQFSSVFEE
                     IDVQLSQTDEFVHILQEEDSFPSDNFYDVRPVLHRIRVAGSWIDQNALSELLKSMLTI
                     TSIVTFFKNDEEKAERYPHLMLLAKEVFVSSEISKKAERIIDEFGQIRDHASPQLSNI
                     RREITSTLNSISRSLNSILRKAQAEGFVDKDITPSMRDGRLVIPVNPAFKRKIKGIVH
                     DESASGKTVYIEPSEVVEANNRIRELESDERREIIRILTEFTDYLRPFLPDLLQSYEF
                     LAQIDFIRAKAKFALQLNALKPNFENRLIIDWVQAVHPLLFLSLKKQNRKVVPLDITL
                     EEDSRILVISGPNAGGKSVCLKTVGLLQYMVQCGLLVPLRENSQVGLFSDIFIDIGDE
                     QSIENDLSTYSSHLQNMKFFVKNCSKHSLLLIDEFGSGTEPQIGAALAESLLDRFNQK
                     NAFGVITTHYQNLKHFANENEGVINGAMLYDRHEMQPLFQLSIGNPGSSFAVEIARKI
                     GLPDDVIAQASEIVGSDYINMDKYLQDISRDRRYWERKREEVRRERNRLNEISEKYES
                     EMEEINRKKKEILAEAKQQAERLIAESNAKIESTIRQIKEAQADKEKTKQVRQSLSEF
                     RENIVASSDTHQEVNPIRKKVKQKPVTQPQNTNLKTPIVTGDTVRMKGQTVTGEILEI
                     QGKKAIVAFGVIKSTVDLEKLEKVSRNQRAKENKSSNTRDLLHDRKLNFRQDIDVRGM
                     RGDEALQAVVYFIDDAIQLSVSRVRILHGTGTGALRQIIRDYLRTVPGVAHFQDEHVQ
                     FGGAGITVIDLD"
     gene            4341..5726
                     /locus_tag="PLI30_05385"
     CDS             4341..5726
                     /locus_tag="PLI30_05385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009135283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOI79022.1"
                     /translation="MLKFSKPFWVANFVELLERLAFYAVFIVITLYLSNVWGFSDVQA
                     GIISGIYSAFLYLLPTFTGAVADKIGFRNSIILAFTFLTIGYGGLGLVPTILQSAGLV
                     HYGMTTTYSGLIGSLHQWSIIPILVFIVIGGAFIKAVISGTVARETTTENRARGYAIF
                     YMMVNIGAFLGKTVVDPLRRSMGDEGLVYLNYFSASMTFLALIAVFFFYKSAHTEGQG
                     KKMQELLQSMGKVLTNGRLMILILIISGFWMIQSQMYSTMPKYVIRLVGDGATPGWYA
                     NVNPLVVFISVNFITSMMKKRNAITSMMAGMFIIPLSALVMSFGNHIGDAYILGMHPV
                     AFMMIIGIAMQALAECFISPRYLEYFSLQAPKGEEGLYLGFSHLHSFFSYLFAFGISG
                     FLLEKYCPDPRLFTSAEGILDKAAYSAATEHAHYIWYVFVGIGAISAVALFIYSRVVK
                     HLDRKLELVKQ"
     gene            5828..7831
                     /locus_tag="PLI30_05390"
     CDS             5828..7831
                     /locus_tag="PLI30_05390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007658847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HOI79023.1"
                     /translation="MRLDLSSYITLERIPQRYYKPENDFEEYTLSRFEKIPIHIFEKS
                     DRAAKKVASGIVKNLQKHQAEGKQFTLGVSGGSSPIQVYEELIRLHKEEGVSFNNLVV
                     FNIYEFYPVSEPSLTNLQFIKDSFLNHIDIDPGNIYSIDATIEKDLIAEKCEEYEQAL
                     HQLGGLDYLLLGLGSKGNIGFNMPGSNLHSQTRLVMLDGDSRRDIASSYESLDKVPVS
                     AITMGIADMMRAKRIALIAWGEQKAESIKQMVEGSVTESTPASVLQTHPEADVYIDLA
                     AASDLTRISQPWLVTNCEWTSKLIRRAIVWLCGIVNKPILKLTNKDYNDNGLSELITL
                     YGSAYNVNIKIFNDLQHTITGWPGGKPNADDSNRPERAKPYPKRVIIFSPHPDDDVIS
                     MGGTFQRLVNQGHDVHVAYQTSGNIAVGDEEVIRYLSVFKNLIKEFEPTNSTALSKLS
                     NILHHLFHEKGVDDIDTPEVRFLKGRIRREEARSADRYVGLPHSHVHFLDLPFYETGR
                     VKKDPISERDVIIVKDFIESIKPHQIYVAGDLADPHGTHKVCLDAILASIDLMKDDEW
                     YKDCRVWMYRGAWMEWEIDHIEMAVPISPEELRQKRNAILRHQSQMESAPFLGDDERL
                     FWQRSEERNRATANMYDKLGLASYEAIEAFVEYKPFRENIENH"
BASE COUNT         2314 a         1871 c         1802 g         2017 t
ORIGIN      
        1 taaaggaggg ttctgaatta tgctaaccat ttttcatctt acttatatcg aactcacatt
       61 taccacaatc aaaactatat ataagagagg tgtgaggttg aaaaacattc taccctatat
      121 attagaaagg catttacagt ccttatgagg ggttttacaa ttagtttaag gaaggattgt
      181 caacagaaag aagcacctct tccagaagca ggaatattgc cctctggcgt cagaaaataa
      241 ccggacggat ctcttaaaag agacagctct tatccaaaca taactattct ctcttttata
      301 ctggagtttt tgtcttgtgg aagtaaaatc ggaagaattt accaattcga tagatttact
      361 ttgtgggtaa aaatagaaga caaagaaagc tattaataaa tgatttgttt gactacggtt
      421 tgactaaaat aataaaacgg tttcaagata tttcctaaaa ccgcttatga aattggtagc
      481 gagagggggg catgatcccc cgacctcatg attatgaatc atgcgctcta accaactgag
      541 ctatctcgcc aacatgtctt tccgaaaatg attccaaatt cgaggtgcaa aagtagttta
      601 aatattttaa ttttgcaacc ctaaaacaat aaaacccgac aaaatattat tttttattcc
      661 aatgccatca gcgcaaacga tgccagccaa tgttctccgc cgtagttccc gtcaaaaaca
      721 agcggaagag catggtccaa tagtttctgg gcggtttctt cgaaatgcag cttcaacgga
      781 tgcccttcgg ataatgattt ggcgattcct ttcatgcacc acgcccgcga aaacgacagt
      841 ccaaccagat gaaccgtctg ataatcatcc aaatcgctta ccacaggaat ctgactgatg
      901 ttatcgatcc ctttttgtgt ataaaaggca accagccact tttcaaaatc gtcccgggaa
      961 agaattctcc tcatcagatc agcaatttcc aggctgggtg agaaaaaatc ggcgccgtcc
     1021 ggctccaggt gtgccggggt ctccgtgtct tttccgtaaa aatcgagcga tttaccgatc
     1081 aactcttttt caaactcctt atttccgttc tcccttgccc aatctatggc aaaacccagt
     1141 gcaaaagctg aattcggatg cactcctgtt ctgttcggat aggtctgctt gggcagatac
     1201 tccctccaca gctccacaat ttgcccggta agcggctgaa ggttttcgtg ccattgcctg
     1261 gcagccggat tgtcccacga cgccagttct tcatcgagtt tcagcagcca cgcccaaccg
     1321 taggttcgtt caaatccttt acctgtctcg tacttgagga aatactcctt ctcaacttca
     1381 accgctgctt tatcaaagga gttattcaac aaggtgatga tttcattttt gctcgcaata
     1441 ccgggtttgg tcttcagtaa gcggatcaac atccaatgcc cgtgaacact actgtgccag
     1501 tcaaaacacc cgtaaaaaac aggatgcagt gccttgggcg aaagacctac ctcatcggcc
     1561 gaaccgtagg tatgtcccgg tttgttggga tattcccgat cgatacaacc gaggggcagg
     1621 gcagataatt taagcgctgt ttcatcggtg agatccggat ttttttgcga aaagagaggt
     1681 gacagcacca tgcttaaaaa aaggttgata aacaaaatgg aggacaacgg tttcataaaa
     1741 tttctgattt atccattcaa agataggaat aatatcggct aaaaagagta attttgcacc
     1801 aaatccgaaa tcgcaaatcc caattccaaa actaaccgtg atttatcccg aaaatttcga
     1861 acaaaaaata gagtttttca agataagaca actgctagaa caacattgcc tcagtccact
     1921 gggcaaagag aaggtagaag agatgcagtt ttcttccgtt tttgaagaga ttgacgtaca
     1981 actatcgcaa accgatgagt ttgtgcatat cttacaggag gaagactctt ttccttcgga
     2041 caacttttac gatgtgcgcc cggttctcca tcgcatacgt gttgccgggt cgtggattga
     2101 tcaaaacgct ttgtcggagc ttcttaaatc gatgttaacg ataacttcca tcgtaacgtt
     2161 ttttaaaaac gatgaagaga aagcggaacg ttatccccac ttgatgttgc tggcaaaaga
     2221 agtgtttgta tcgtcggaaa taagcaaaaa agccgagcgg atcatcgatg agttcggaca
     2281 aataagggat catgcatctc cccaactaag caatatccgc agggaaatca cctcgacgct
     2341 caacagcatc tctcgaagcc tgaattcgat attacgcaaa gcacaggccg agggctttgt
     2401 ggacaaagac atcactccca gcatgcgcga cggaaggttg gttatccccg taaatccggc
     2461 tttcaagcga aaaataaaag gaattgtcca cgacgagtcg gcctcaggga aaaccgttta
     2521 tatagaacct tcggaggtgg tagaggccaa caaccgcatc cgtgagctgg aaagcgacga
     2581 acgtcgcgag atcatccgca tcctcaccga gtttacggat tacctccgcc cttttctacc
     2641 cgacttgctg cagtcctacg aattcctggc acaaatagat ttcatccgcg ccaaagccaa
     2701 atttgcgctg cagctaaacg cattgaaacc caacttcgaa aacaggctca ttatcgactg
     2761 ggtacaagcg gttcatccgc ttctttttct ttcgctgaaa aagcaaaacc gcaaagtggt
     2821 accgctcgac ataacgctcg aagaggacag ccggatactg gtgatctcgg ggcccaatgc
     2881 cggaggaaag tcggtttgtc tgaaaaccgt aggactactg caatatatgg ttcaatgcgg
     2941 cttgttggtt ccgttacggg aaaattcaca agtgggcttg ttcagcgata tttttattga
     3001 tataggcgac gagcagtcca tcgagaatga cctctccaca tacagctccc acctgcagaa
     3061 catgaagttc ttcgtgaaaa actgcagtaa gcattctctc ttactgatcg acgaatttgg
     3121 cagcggcacg gagccacaaa ttggagcagc ccttgccgaa tcgctgttgg atcggtttaa
     3181 ccaaaagaac gcttttgggg tcatcaccac ccactaccag aacctaaaac attttgccaa
     3241 cgaaaatgaa ggggtcatca atggagccat gctttacgac agacatgaaa tgcagccgct
     3301 cttccagctt tccatcggaa accctggaag ctccttcgct gtagagattg cccgcaagat
     3361 cggacttccc gacgatgtaa tcgcccaagc ctccgaaatt gtcggcagcg attacatcaa
     3421 catggataaa tatttgcagg acatttcccg tgaccgccgt tactgggagc gtaaacgcga
     3481 agaggttcgc cgcgaacgca accggcttaa cgagatttcg gaaaaatacg aatccgaaat
     3541 ggaggaaata aaccggaaga aaaaagaaat attggccgaa gcaaaacagc aggctgaacg
     3601 gcttatagct gagagcaatg caaaaattga aagcaccata cgccagatca aggaagcaca
     3661 ggcagacaag gaaaagacca aacaggtgcg ccaatcgctc agtgaattcc gggagaacat
     3721 agtagcgtcc tccgacactc accaggaagt gaatccgatc cgaaagaaag tcaagcaaaa
     3781 acccgtaact caacctcaaa acaccaacct caaaacgcca atcgtcactg gcgataccgt
     3841 tcgcatgaaa ggacagaccg ttaccggaga aatcctggag atacagggaa aaaaagcgat
     3901 cgttgccttt ggagtaatca aatccacggt cgacctggag aaactggaaa aggtgagccg
     3961 taatcagcgg gccaaagaaa ataaatcgtc caacacgcgc gatctgctgc acgaccgcaa
     4021 gctcaacttc aggcaggata tcgacgtacg cggaatgcgt ggtgacgaag cattacaagc
     4081 tgtagtgtac tttatcgacg atgccattca gctcagcgtt tcccgggtac gcatcctgca
     4141 cggaacaggc accggcgcat tgcgacaaat catccgggac tacctgagaa cagtaccggg
     4201 tgtcgcccat tttcaggatg aacatgttca atttggcggt gccggaatca cggtaattga
     4261 tttggattaa acattattgt tttatctttg cacgaaaaac aaccatttgc gcactaaatt
     4321 aacagataaa acaaactctt atgctgaaat tttcaaaacc tttttgggtc gcgaactttg
     4381 tagaactcct tgaacgtctg gcattctacg ccgtctttat tgtaatcacc ctctatctgt
     4441 ccaacgtctg gggtttctcc gatgtgcaag caggcatcat ttccgggata tattccgctt
     4501 ttttgtattt acttccaacc ttcaccggag ccgttgccga caaaataggc tttcgcaatt
     4561 ccatcatcct ggcatttacc ttcttaacca tcggttacgg tgggctgggc ctggttccca
     4621 caatattgca gagcgcaggg ttggtacatt acggaatgac caccacctat tcgggattaa
     4681 tcggaagcct acaccaatgg tcgattatcc cgattcttgt ttttatcgtg atcggtggcg
     4741 ctttcataaa ggcagtaata tcgggaaccg tggctcgtga aaccactacg gaaaatcgcg
     4801 cccggggata tgccattttt tatatgatgg tgaatatcgg ggcatttttg ggtaagaccg
     4861 tggtcgaccc tctacgaagg agtatggggg atgaaggcct ggtctacctc aattactttt
     4921 ccgcttccat gacattttta gcactgattg ccgtgttctt cttttacaaa tcggcgcata
     4981 ccgagggtca gggaaagaaa atgcaggagc ttttgcaatc gatgggcaag gtgctgacaa
     5041 acggaagatt gatgatcctt atcctgatca taagcggttt ttggatgatc caaagccaaa
     5101 tgtactctac aatgccgaaa tacgtcatac gactggtagg cgatggtgct acaccgggat
     5161 ggtatgccaa tgtgaatcca ctggtggtat ttataagcgt taattttatt acctccatga
     5221 tgaaaaaaag aaacgccatc acttcgatga tggcaggcat gtttattatc ccgctttcag
     5281 cgttggtcat gtcttttggg aaccacatag gagatgcata tatcctgggg atgcatcccg
     5341 ttgcattcat gatgattatc ggtattgcca tgcaggcgtt ggccgaatgt tttatttcac
     5401 ctcgctattt ggagtatttt tcgctgcaag cacccaaagg cgaagaggga ttatacctgg
     5461 gattcagcca cctgcattca ttcttctcct acttgtttgc ttttggaata tcgggattcc
     5521 tgctggaaaa atattgcccc gatccacgcc tgtttaccag tgccgaaggc atactcgaca
     5581 aggcagccta ttctgcggca acagagcacg cacattatat ctggtacgtt tttgtaggaa
     5641 ttggagccat ttctgcggtt gccctcttta tctattccag ggtagttaag cacctggata
     5701 ggaagcttga acttgtgaag caataaattt actttattac ttattgatca atatttaata
     5761 attactttgc tttaattgcc tattttgtct atttttgtca atggaaattc tttataaaaa
     5821 tcatgtcatg cgattagatt taagttctta cattacactc gaaagaatac ctcaacggta
     5881 ttataaaccg gaaaacgatt ttgaggaata cacgctcagc cgcttcgaaa aaatcccgat
     5941 tcacattttt gaaaaatcgg atcgtgctgc aaaaaaagta gccagcggca ttgtaaaaaa
     6001 cctgcaaaaa catcaggcgg agggaaaaca gtttaccctc ggcgtatccg gaggatcttc
     6061 acccattcag gtatatgaag aactgatcag gctgcataaa gaagaagggg tgagcttcaa
     6121 caacctggtg gtatttaaca tttacgagtt ctatccggtt tccgaaccat cccttactaa
     6181 tttacagttc atcaaggact cttttttgaa ccacatcgac atagaccccg ggaacatcta
     6241 ctcgatcgat gccacaatcg aaaaggacct catcgcggaa aagtgtgagg agtacgaaca
     6301 ggcattgcat caattggggg gattggatta tttgctgctg ggattgggaa gcaagggaaa
     6361 catcggcttt aacatgccgg gtagcaacct ccattcacaa acccgcctgg tgatgctcga
     6421 cggagactcg cgacgagaca tagcttccag ctacgaatca ctggacaaag tgcccgtcag
     6481 cgctatcact atgggtattg cggatatgat gcgagcaaag agaattgcac tgattgcctg
     6541 gggcgagcaa aaagcagaaa gcatcaaaca aatggtggaa ggaagcgtaa cggaatcgac
     6601 gcctgcgtca gttttgcaaa cgcatccgga agcagatgtt tatatcgatc tggctgcagc
     6661 cagcgacctg acccgtatca gccagccttg gcttgtaacc aattgtgaat ggacaagcaa
     6721 acttatccgc agagcgatcg tttggttgtg cggcattgtg aacaaaccca ttttgaaact
     6781 caccaacaag gactacaacg ataacgggct aagcgaactt ataacccttt acggttctgc
     6841 gtacaacgtc aacataaaaa tattcaacga cctgcaacac accatcaccg gctggccagg
     6901 cgggaaaccc aatgccgacg attccaaccg tccggaaaga gcaaaaccgt atcccaaacg
     6961 cgtgatcatc ttcagccctc accccgatga tgatgtgatt tctatgggtg ggacctttca
     7021 gcggttagtg aatcaagggc acgacgtgca tgttgcttac cagacttctg gaaacattgc
     7081 cgttggcgac gaagaggtta tccggtattt atcggtcttc aaaaatctca tcaaagagtt
     7141 cgaaccgacc aactctaccg ctcttagcaa gttatcgaac atattacacc atctcttcca
     7201 cgaaaaaggg gtagacgata tagacacacc tgaagttcgt tttctcaaag gaaggattcg
     7261 ccgtgaagaa gctcgctctg cagacagata cgtggggtta ccacacagcc atgtacattt
     7321 tctcgacttg cccttttacg aaaccgggag ggtaaaaaaa gaccccattt cggaaagaga
     7381 cgtgataatc gtcaaggact ttattgaaag catcaaaccg catcaaatct atgttgccgg
     7441 cgacctcgcc gatccgcacg gaacccacaa agtatgtctg gatgctattc tggcctccat
     7501 cgatttgatg aaggatgatg agtggtacaa ggattgccgc gtttggatgt accggggtgc
     7561 ctggatggaa tgggaaatag accatatcga gatggccgtt ccaatctctc ccgaagagtt
     7621 acgccagaaa agaaatgcta tcctgcgcca tcagtcgcaa atggaaagtg ctccgttcct
     7681 cggtgacgac gaacggttgt tctggcaacg ttctgaagag cgtaaccggg caaccgccaa
     7741 catgtacgac aagctcggat tggcctccta tgaagccatt gaggcattcg tggaatacaa
     7801 gccttttcgg gaaaacatcg agaatcattg acggataagg gaactttttc tcagtataag
     7861 gcgcgtcata ccagtttttg ttccagcgct atccgcacca gaacggctgt gttacgagcg
     7921 ttaagcttac aaagaagctt ctttcggtag ttattgatgg tatctacggc aaggagcatc
     7981 tgttctgcaa tttcaggatt ggtg
//
LOCUS       DAOSDR010000100         8003 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrimonas sp. isolate AMR_MDS_1826
            NODE_3346_length_8003_cov_3.862229, whole genome shotgun sequence.
ACCESSION   DAOSDR010000100 DAOSDR010000000
VERSION     DAOSDR010000100.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863355
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrimonas sp. (wastewater metagenome)
  ORGANISM  Petrimonas sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Dysgonomonadaceae; Petrimonas.
REFERENCE   1  (bases 1 to 8003)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8003)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:10:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,720
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 35
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8003
                     /organism="Petrimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3346_length_8003_cov_3.862229"
                     /isolate="AMR_MDS_1826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2023866"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            569..1687
                     /locus_tag="PLI30_05395"
     CDS             569..1687
                     /locus_tag="PLI30_05395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006843485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HOI79024.1"
                     /translation="MKKNAKISLFTVIGLLILGMAFFPKIKSLFSSGNPQTENQGPPR
                     GGGGGGAGRQQLSVTAMVLQPQVLRSDLYIARGILLPEEEVDLTFETSGKITHIYFRE
                     GSFVTQGALLAKVNDAPLQAELRKLQTQLPLAQDRVYRQGTLLEKDAISQETYQTVTT
                     ELEKLKADIDLIESRIAQTELRAPFSGVIGLRQVSVGAYASPAVIVSKLTKISPLKLE
                     FSMNENQVSQIPPGAKITFTVANDLTTYEAEVYAVESQLDPKTLTLKARARYANPGGK
                     LKPGISASIQTLISEIPDAIVIPSISSITEMGRDIAYIYKNGQAQEVEITKGYRTASS
                     VQVLSGLSAGDTLLTTGVMQLRNGLPVKISEIISKPTD"
     gene            1692..4745
                     /locus_tag="PLI30_05400"
     CDS             1692..4745
                     /locus_tag="PLI30_05400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009599056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HOI79025.1"
                     /translation="MTLSELSINRPVLATVLVLIIIIFGVIGYTSLAVREYPNVDNPI
                     ITVSVGYPGANAEVIENQITEPLEQNINGIPGIRSLMSQSSQGNSRITVEFELSVDLE
                     TAANDVRDKVSRAMRYLPRDADPPTVAKADADADPIMQVTVESPTRSLLQLSEIAELT
                     VKERLQTISNVSAVEIWGENRYSMRLWLDPVRMASYNITPMDVKNALDRENIELPAGS
                     IEGDHIEQSIRTMGLMQTPEEFNNMVIIEDDYRIVRFGDIGTAELDTENRKNILRRNG
                     IPMVSCVIIPQPGANQIVIANEAALRIDQMRKDLPEDIKLDVAFDNTRFIRASISEVQ
                     TTVLVAFVLVVLVIFLFLRNTRVTLIPTLVIPISLIGTFFIMYLAGFSINVLSMLAVV
                     LSVGLVVDDAIVVVENIYAKVEQGMSPRDAAVEGSKEIFFAVISTTITLVAIFFPIIF
                     LEGMTGRLFREFSIVISGAVIISSFVALSFVPMLSSKLLKKKEKHDKIYEKTEKFFQG
                     MNAFYNRSLKAFLHRRWLIYPVIGVALVLIVVLWNTIPAEMAPLEDRSQIMIRTSTPE
                     GATFEFTRDYTEGISRIADSVAWERESNITMIRGTFGMVRLVLPDIKERERSQMEVAD
                     ALSAAVRNQTAARAFVQQQSTFGGRRGGMPIQYILQAPNIEKLQEFIPPFMEKVNQSP
                     YFQMADVNLKFTKPETRIVIDRDKAALLGVSTRDIGQTLQYALSGQRMGYFYMNGKQY
                     QVLGEINRQQRNTPLDLKTIYLKNSSGQMIQMDNLVKLEETVAPPQLYRYNRFNSATV
                     SAGLAQGYSLGEGLEEMDRIAAEVLDETFRTALEGESRNFRESSSSLLFAFGLAIILI
                     FLVLAAQFESFKDPFVIMLTVPLAVFGALLFMWLFGVTMNIYSQIGIIMLIGLVAKNG
                     ILIVEFSNQRQAAGMNKYDAVMGASIQRLRPILMTSISTILGLLPLAMATGEGANGRI
                     AMGIAVVGGMLISTLLTLFVVPAMYMYVSTSRKSKTKENIEVQHV"
     gene            4738..6072
                     /locus_tag="PLI30_05405"
     CDS             4738..6072
                     /locus_tag="PLI30_05405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006799293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HOI79026.1"
                     /translation="MFKLKRFALPAIVLLLPFLSAAQAVIDLNEVIRIGLENNYDLKI
                     SRNEQQISDNNLTLGNAGFLPSVGLNSGYSVRENNSDQFPADGGEAVTTKNSVTQTLD
                     AGLNLNWTIFEGFKVQTNYKRLQELKSVGELNTQLAVENFIADISAEYYNYVQQTLRM
                     RNLRQALNLSRERLRIVEARYQIGSLSRLDLQQARVDFNADSSQLIQQYEVLHSSRIL
                     LNEMMGTGNVEQHFMAADTTISFDPMLSKLALYDNMMKVNTALLLTEKNKVLSEFDLK
                     TLQSQNYPYLRLNGGYGFTHYDYTKGNFDKQRTWGPTVGVTLGFTIFDGFNRSREQKN
                     ARIRIDNRELQVERQKLMLRSDFANMWLAYQNNIQLTNLEKESLENAQINYEIAMDRY
                     KLGDLSGILLREAQVSLLQAEQRLLTAQYRTKLYEISLLQISGRIGEYLSTH"
     gene            complement(6061..7527)
                     /gene="gltA"
                     /locus_tag="PLI30_05410"
     CDS             complement(6061..7527)
                     /gene="gltA"
                     /locus_tag="PLI30_05410"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008779314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004355 - glutamate synthase (NADPH)
                     activity [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent glutamate synthase"
                     /protein_id="HOI79027.1"
                     /translation="MPTQEYLQAERAQLWREELRKTLKNKERTNLVRVKMPEVDATIR
                     SRTYDEVNLGLTPEQAQAESQRCLDCVNPTCITGCPVEINIPKFIKNIERGEVLEAAK
                     ALKETSALPAVCGRVCPQERQCESQCFYTLKLGKEPVAIGHLERFAADFERKSGNISI
                     PEIAPANGIKIAVVGSGPAGLAFAGDMIKLGYDVTVFEALHELGGVLRYGIPEFRLPN
                     DIVDIEIDVLKKMGVKFITNCIIGKTISQEELKADGYRGIFIGSGAGLPNFMNIPGEN
                     LIGVMSCNEYLTRVNLMQAADPESDTPVQMGKKVAVIGGGNTAMDAVRTARRLGAEKA
                     MIVYRRSEEEMPARLEEVKHAKEEGINFLTLHNPIRYEGDERGHVQRMLLQRMKLGDP
                     DSSGRRKPVVIEGDTVWVDVDEVIVSVGVSPNPLIPSSFSGLEITSWGTVVVNSDTMQ
                     TADETIFAGGDIVRGGATVILAMGDGRKAAKAMHASMS"
     gene            complement(7572..>8003)
                     /locus_tag="PLI30_05415"
     CDS             complement(7572..>8003)
                     /locus_tag="PLI30_05415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006842004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfide/dihydroorotate dehydrogenase-like
                     FAD/NAD-binding protein"
                     /protein_id="HOI79028.1"
                     /translation="EAMKNAGNRVITVLAARNKDLIILEDQVRKFSDEVIVMTDDGSY
                     GQKGLITAGVEQVILREPVDLCVTIGPAIMMKFVSELTKKYSVPTVASLNTIMVDGTG
                     MCGACRVTVGGVTKFVCVDGPEFDAHQVDFDEMLMRLKAYN"
BASE COUNT         2083 a         1878 c         1985 g         2057 t
ORIGIN      
        1 gggagaaatc agagataact tgaatctgaa aacaaatccg ggaaatctgg gcaaagaggt
       61 aaaaataaaa ggaaaaattg gtacttatta cggtgcgatg ggtatacccg atgctactgc
      121 ttatgtgttt attgttgatc attagtatgc agctaaatat gagaactgta catgttttgt
      181 ggaacgccta tgctatgaaa caggcatgat cggatatgct gtccgatgaa acttaaatta
      241 tttcggtttt caaaacaaaa acaggttttg ggctgtttta tcgggtaaaa aaatatctgt
      301 atgggcaaga ctgcttgcaa agaaaaagat tttgaagaag ccaaagagcc gaaatacgtt
      361 tgtaagaaat gtggagcaaa ggttaagaag gaaaaacaag cctgtaaacc caggaaaatt
      421 tagcgggctc tcgagtttga tttattgatt ttcgtcgatg gatttgtccg gtctgtcgat
      481 tttgaatttt aaaaaagact aaaaagaacg tccgcttcct cttgttgtgg cttttaatcg
      541 ctaaatttgg ccaaatttgg actgaaaaat gaaaaaaaac gccaaaattt cactatttac
      601 agttattggt ttactgattt tgggaatggc ttttttcccg aaaataaaat cgcttttttc
      661 ttccggtaat ccacaaacag aaaatcaggg accgcctaga gggggtggcg gaggaggagc
      721 aggaaggcag cagttgagtg tgactgccat ggtcttgcaa ccgcaggtcc tgcgaagtga
      781 cctctatatt gcccggggca ttttattgcc cgaggaagaa gttgacctca cttttgaaac
      841 ctccggtaag attacccata tttattttcg agaagggtcg tttgttactc agggagcctt
      901 gctcgccaag gtaaacgatg cccctttaca agccgaatta agaaaacttc agacgcagct
      961 tcctttggcg caggatcggg tttacaggca ggggacactg ctcgaaaaag atgccatcag
     1021 ccaggagacg tatcagacgg taaccactga actggaaaag ttgaaggccg atatcgacct
     1081 gattgaatct agaattgctc aaaccgagtt aagggcgccg ttcagcggag ttatcggatt
     1141 gcgccaggta agtgtgggag catatgcatc acctgcagtg atcgtttcaa aactgacgaa
     1201 aatttcgccg ttgaagctgg aattctccat gaacgaaaat caagtcagtc agatacctcc
     1261 cggagcaaaa ataaccttta cggtagccaa cgacctaacg acatatgagg ctgaggtata
     1321 tgccgtggaa tcgcagttgg atcccaagac gctgacgttg aaagcccgcg cacgctatgc
     1381 taatccgggc ggaaagctga aaccgggcat ttctgcctcc attcagacat taataagcga
     1441 aattcccgat gccatagtta ttccgagcat ctcaagcatt accgaaatgg gaagggatat
     1501 tgcctacatt tataaaaacg ggcaggcaca ggaagtagag ataaccaaag ggtaccggac
     1561 agcatcttcc gtacaggtgc tttccggact atctgccgga gatacattac ttacaaccgg
     1621 tgtgatgcag ttgagaaatg gattgccggt aaaaatttca gaaataattt ccaaaccaac
     1681 ggactaacag tatgacctta tccgaactca gtataaatcg tccggtttta gccacggtgt
     1741 tggtgctgat catcatcatt ttcggggtga tcggatatac cagcctggcc gtacgcgagt
     1801 atcccaacgt ggataatccc atcattacag tatccgtggg gtatcccggt gcaaatgccg
     1861 aagtgatcga gaaccagatt acagagccgt tggaacaaaa catcaacggg atccctggta
     1921 ttcgctcgct gatgagccaa agcagccagg gaaactcgag aataactgtt gagtttgaat
     1981 tgagtgttga tttggaaacg gcggccaacg atgtacgcga caaggtttcc agggctatgc
     2041 gatatttgcc tcgtgatgcg gatcctccaa cggttgccaa ggccgatgcc gatgccgacc
     2101 cgattatgca ggttacggtc gaaagcccca cacgctcgct gttgcaattg tccgaaatag
     2161 ccgagcttac cgtgaaggaa cgtttgcaga ccatctccaa cgtgagtgcg gtggagattt
     2221 ggggagaaaa ccggtattcg atgcgcttgt ggctcgatcc ggtaagaatg gcatcctata
     2281 acattacccc catggatgtg aaaaatgccc tggacaggga aaatattgaa ctgccggccg
     2341 gaagcatcga gggagaccat attgagcaat ctatccgcac catggggctg atgcagaccc
     2401 ctgaagagtt taacaacatg gtgattatcg aggacgatta ccggatcgta aggtttggcg
     2461 atatcggaac ggcagaactg gatacggaaa accggaaaaa tatcctcagg cgtaacggta
     2521 tacctatggt cagctgtgtg atcattccgc aacccggggc caatcagatt gtgattgcca
     2581 acgaggctgc attgagaata gatcagatgc gcaaggactt gcctgaagat attaagctcg
     2641 atgtcgcttt cgataataca cggtttatcc gtgcgtccat ctccgaagtg caaacgacgg
     2701 ttttggttgc ttttgtgctg gtggtgctgg taatcttcct cttcctgaga aatacccgtg
     2761 taacgcttat tcccacactg gttatcccca tctcgcttat cgggacattc tttatcatgt
     2821 atttggcggg attttccatc aacgtgcttt ccatgcttgc ggtggtgctt tccgtgggac
     2881 tggtggtgga cgatgccatc gtggtagtag aaaacattta tgcgaaagtg gaacagggaa
     2941 tgtctcccag ggatgcggct gtggaaggtt cgaaagagat attttttgcc gttatatcca
     3001 cgactattac gctggtcgcc atattctttc cgataatctt cctggaagga atgacgggac
     3061 gcttgttcag ggagttcagt atcgttattt cgggagcggt aataatttcg tcttttgttg
     3121 cactgtcctt tgttccgatg ctctcgtcga aactgctgaa aaagaaagaa aaacacgata
     3181 aaatctatga aaagaccgag aagttcttcc agggaatgaa tgctttttac aataggtcgc
     3241 tgaaggcgtt tcttcaccgc aggtggttga tttatccggt tattggggtg gcgttggtac
     3301 ttattgtggt tttgtggaat accattccgg ccgagatggc tcccctggaa gaccgatcac
     3361 aaattatgat ccggacttct accccagaag gggcgacttt tgagtttaca agagattata
     3421 ccgaaggtat ttcacgaatt gcagattccg tggcctggga aagagaatca aacattacca
     3481 tgatcagggg tactttcgga atggtccggc ttgttttacc ggacattaag gagcgcgaaa
     3541 gaagccagat ggaagtggcc gatgcgttgt ctgctgcggt tagaaaccag actgcagccc
     3601 gtgcgtttgt gcagcagcag tccacttttg gcggccgtcg tggcgggatg cctatccagt
     3661 atatcttgca agcgcccaac atcgaaaaac ttcaggagtt tatccctcca tttatggaaa
     3721 aggtgaatca aagcccttat tttcagatgg ccgacgtcaa cctgaagttc accaagcccg
     3781 aaacccggat cgtgatcgac agggataagg cagctttgct gggcgtaagc acccgcgata
     3841 tcgggcaaac cttgcaatat gcattaagcg gccagcgcat gggttacttt tacatgaacg
     3901 gaaaacaata tcaggtgctg ggagaaataa accgccagca acggaacaca ccgcttgatc
     3961 tgaaaaccat ctacctgaaa aattcctcgg gacagatgat ccaaatggac aacctggtca
     4021 aactcgaaga aaccgttgct cccccgcagt tgtaccgata caaccggttt aactcagcaa
     4081 ccgtttcagc cggcttggca caagggtatt cgctgggcga agggttggaa gagatggaca
     4141 ggattgcggc agaagtgctg gacgaaacct ttcggacggc gctggaaggc gaatcgagaa
     4201 actttcgtga aagctcttcc agtttgcttt tcgctttcgg gctggccatc atacttattt
     4261 tcttggtatt ggccgcacaa ttcgagagtt tcaaagaccc gttcgtaatt atgcttaccg
     4321 ttccgttagc tgtttttggt gccttgctct ttatgtggtt gtttggggtt accatgaata
     4381 tttacagcca gatcgggatc attatgttga tcggtttggt cgccaagaac ggaatcctta
     4441 tcgttgagtt ttccaaccag cgccaggcag cgggaatgaa caagtacgac gcggtgatgg
     4501 gcgcctccat ccagcgttta cgcccgatat taatgacaag tatatccacg atactggggt
     4561 tgttgccgct ggcaatggcc accggagaag gagccaacgg acgtattgca atgggtattg
     4621 ccgtagttgg cggcatgctt atttccacac tgttaacgtt gtttgtcgtt ccggccatgt
     4681 acatgtatgt atccaccagt agaaaatcga aaacaaaaga aaacatcgaa gttcaacatg
     4741 tttaaattaa agagattcgc tcttccggct attgtgcttc tgctcccttt tttatcggct
     4801 gcacaggcag ttatagattt aaatgaagtg atacgtatcg ggttggagaa caactacgac
     4861 ctgaaaatca gcaggaacga acaacagatt tccgacaaca acctgacgtt gggtaatgcc
     4921 ggattccttc cttcagtggg actgaattca gggtatagtg ttcgggaaaa caactccgat
     4981 cagtttccgg ccgatggagg cgaggcagtc acgacaaaaa actccgtgac acaaacgctt
     5041 gatgccggat taaacctgaa ctggaccatt tttgaaggat tcaaggtcca gacaaactat
     5101 aaacgcctgc aggaactgaa atcggtgggc gaactgaata ctcagctggc ggtggagaac
     5161 tttattgccg atatttcggc tgaatactac aactatgttc agcaaacgtt acgcatgcgg
     5221 aatttacgcc aggcgctgaa cctgtcacgt gagcggctgc gaatagtgga agcgcgttac
     5281 cagataggtt cactctcccg gctcgacctg cagcaggcgc gcgtggattt taatgccgac
     5341 agttcgcaat tgattcagca atacgaggtt ttgcacagtt cgcgcattct gttgaatgag
     5401 atgatgggta caggtaatgt tgaacagcat tttatggctg ccgatacaac catctcgttt
     5461 gacccgatgc tatctaaact ggcactttac gacaacatga tgaaagtaaa taccgcactg
     5521 ctcctgaccg aaaaaaacaa ggtgctgagc gaattcgatt tgaaaacgct gcaaagccag
     5581 aattatcctt acctgaggct gaacggcgga tacggattca cacactacga ctacaccaaa
     5641 gggaatttcg acaagcagcg gacctggggc cctacggtag gggttacgct tggttttacc
     5701 attttcgacg ggtttaatag gagtcgtgaa cagaaaaatg cacggatccg gatagacaac
     5761 cgagaacttc aagtggaaag gcagaagctt atgctgcgaa gcgattttgc caatatgtgg
     5821 cttgcctatc aaaacaatat acaactcact aacctcgaaa aggaaagcct ggaaaatgcg
     5881 cagatcaact acgagattgc gatggacagg tacaagctgg gcgacctttc ggggattctg
     5941 ttgcgtgaag cacaggtaag ccttcttcag gccgagcagc gattactcac cgcacagtat
     6001 cgcaccaaac tttacgaaat ttcattgtta cagatcagcg gaaggatagg ggagtacctg
     6061 tcaactcatt gatgcgtgca tcgccttggc tgcttttctg ccgtcaccca tggcaaggat
     6121 cactgttgct ccgccccgga caatatcgcc gccggcaaaa atggtctcat cggcagtttg
     6181 catggtgtct gaatttacca caacggttcc ccacgaagtg atctccaggc ccgaaaaaga
     6241 ggatgggatc agtgggttgg gcgatactcc cacactcacg ataacttcgt ctacgtccac
     6301 ccagaccgta tccccttcga tcaccaccgg ctttctcctg ccggaagaat cggggtcacc
     6361 caacttcatc cgttgcagca gcatccgctg tacatgtcct cgctcgtccc cttcgtaacg
     6421 aataggattg tgtaatgtca ggaaatttat tccttcttct ttggcatgct tcacttcttc
     6481 cagacgtgca ggcatctctt cttccgaacg gcggtaaaca atcattgctt tttccgcacc
     6541 cagccggcga gctgttcgta cggcatccat cgcggtattg cctcctccga taacggcgac
     6601 ttttttcccc atttgtacgg gggtatcgct ttcggggtct gctgcttgca tcaggtttac
     6661 ccgcgtgagg tattcgttac acgacatcac tccgatcaga ttttcgcccg gaatattcat
     6721 gaaattcggc aatcccgcac cgcttccgat gaagataccc cgatagccgt cggctttcaa
     6781 ctcttcctgt gaaatggttt tcccgatgat gcagttggtg atgaacttaa cacccatttt
     6841 tttcaaaaca tcaatctcta tatccacgat atcgttgggc aggcgaaact cggggatccc
     6901 gtagcggaga acgcccccca gttcatgcaa tgcttcaaaa accgtaacgt cgtaacccag
     6961 tttaatcatg tcgccggcaa aagccagccc ggcagggccg gatcccacga cggcaatctt
     7021 tataccgttt gccggagcaa tctcgggaat ggaaatgttg ccgcttttgc gttcaaagtc
     7081 agcggcaaac cgttccagat gaccaatagc tacaggctcc ttgcccagtt tcagggtgta
     7141 aaaacattgg ctttcacact gcctttcctg tggacaaacc ctaccgcaaa ccgcaggtaa
     7201 ggcgctggtc tcttttagcg ctttagccgc ttccagcacc tctccacgtt cgatgttctt
     7261 gatgaacttg gggatgttta tttcaaccgg acaaccggta atacaggtgg gattaacgca
     7321 gtcgaggcac cgttgagatt cggcttgcgc ttgttccgga gtcagtccca gattgacctc
     7381 atcgtatgtg cggcttctga ttgtcgcatc cacttccggc atttttacgc gaacgaggtt
     7441 ggttctctcc ttgtttttta gcgttttgcg caactcttcg cgccacaact gtgctctttc
     7501 tgcttgcaga tattcttgtg taggcattta ttctctgttc tttgctcttt gttacttgtt
     7561 ctttgttctg atcaattata cgctttcagc cgcatcaaca tctcatcgaa atctacttga
     7621 tgggcgtcaa actccggccc gtccacgcag acgaacttgg tgaccccacc caccgtcacc
     7681 cggcaggcgc cgcacatccc ggtgccgtca accataatcg tgttgagtga ggcaacggtg
     7741 ggcacactgt attttttcgt cagctccgat acgaacttca tcatgattgc cgggccgata
     7801 gtaacgcaca ggtcaaccgg ttcgcgcaga attacctgct ccactccggc agtgatcaat
     7861 cccttttgtc cgtatgagcc gtcgtcggtc ataacgatca cttcgtctga aaattttcgt
     7921 acctgatctt ccaggatgat caggtctttg tttctggcgg caaggacggt aattacccgg
     7981 ttgcccgcat ttttcatggc ttc
//
LOCUS       DAOSDR010000101         7908 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrimonas sp. isolate AMR_MDS_1826
            NODE_3379_length_7908_cov_4.330447, whole genome shotgun sequence.
ACCESSION   DAOSDR010000101 DAOSDR010000000
VERSION     DAOSDR010000101.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863355
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrimonas sp. (wastewater metagenome)
  ORGANISM  Petrimonas sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Dysgonomonadaceae; Petrimonas.
REFERENCE   1  (bases 1 to 7908)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7908)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:10:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,720
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 35
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7908
                     /organism="Petrimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3379_length_7908_cov_4.330447"
                     /isolate="AMR_MDS_1826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2023866"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..234
                     /locus_tag="PLI30_05420"
     CDS             <1..234
                     /locus_tag="PLI30_05420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HOI79029.1"
                     /translation="RKEFDKKEIHYLETILADGSKSAVFTVNDIPRTAEILHFALKGL
                     EVPVIRGVLNLRLDRQEDREIVYNLVFKGLNKR"
     gene            269..1015
                     /gene="fabG"
                     /locus_tag="PLI30_05425"
     CDS             269..1015
                     /gene="fabG"
                     /locus_tag="PLI30_05425"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010536417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HOI79030.1"
                     /translation="MKLLEGKTALITGAARGIGKAIALKYASEGANIAFTDLTIDEVG
                     KATESEIASLGVKCKGYASNAANFDETHKVVEEVLSDFGRIDILVNNAGITKDGLMMR
                     MTEQQWDAVINVNLKSAFNFTHAVTPVMMRQKAGSIINMSSVVGVSGNAGQSNYSASK
                     AGMIGLTKSMAKEVGSRGIRVNAIAPGFIITDMTAALSEEVRNEWAKQIPLRRGGTPD
                     DVANTAVYLASELSSYVSGQVIHVCGGMNM"
     gene            1021..1701
                     /locus_tag="PLI30_05430"
     CDS             1021..1701
                     /locus_tag="PLI30_05430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006842921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pseudouridine synthase"
                     /protein_id="HOI79031.1"
                     /translation="MTVLYEDNHLVVVNKAPGEIVQGDKTGDKPLSEEVKEYLKVKYN
                     KPGNVFCGVTHRLDRPTSGIVVFAKTSKALSRLNAMFKNNEIDKTYWAVVKKLPEKTE
                     ATLTHYLIKNERTNKSTAYDVEKPNTKKSVLHYKVIGQSQNYNLLEVDLETGRHHQIR
                     CQLSKIGSPIKGDLKYGAERSNPDGSISLHARKIAFIHPVSKEKIELVAPTPDDVLWN
                     ALINTNVH"
     gene            complement(1723..2130)
                     /gene="arfB"
                     /locus_tag="PLI30_05435"
     CDS             complement(1723..2130)
                     /gene="arfB"
                     /locus_tag="PLI30_05435"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019539571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alternative ribosome rescue aminoacyl-tRNA
                     hydrolase ArfB"
                     /protein_id="HOI79032.1"
                     /translation="MTEPDIDKLMNECTFSTVRSSGSGGQHVNKVETKVTLSLNIGQS
                     QILSTEQKQRIQQKLRNRISQEGILQISSDTERSQAMNKKAVIVKLENLLKKACREEK
                     KRVATKRTLSSIQKRLDNKKQLSEKKRRRSENF"
     gene            2288..3190
                     /locus_tag="PLI30_05440"
     CDS             2288..3190
                     /locus_tag="PLI30_05440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005867990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HOI79033.1"
                     /translation="MKPTLFVLAAGMGSRYGGLKQLDGVGPSGETIMDYSIFDAVNAG
                     FGKLVFVIRQSFEEDFREKIVKKYEKKIPVELVFQELDALPEGFVVPEGRVKPWGTNH
                     AVMMGKEVIQEPFAVINADDFYGRESFGVLADYLKTLENSENKYGMIGYRVGNTLSES
                     GTVARGVCETDENGNLTGVVERTQVKRIDGAVCYREDNGNWIAIDDNTPVSMNMWGFT
                     PDYFNHSDDYFVHFLKENAENTKAEYFIPLLVNHLIVNGVSTVKVLDTPSKWFGVTYA
                     EDRPGVVKKLQELSDEKVYPSPLW"
     gene            3196..4497
                     /locus_tag="PLI30_05445"
     CDS             3196..4497
                     /locus_tag="PLI30_05445"
                     /inference="COORDINATES: protein motif:HMM:NF013741.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HOI79034.1"
                     /translation="MIDVFIIFFLIILNGFFAMSEIAVVSAKKTKLETERKKGNKGAE
                     KALKLQSDPDSFLSSVQVGITLIGIVNGAYGGATLSVYLIPFFDRFAWSAPYAETISM
                     VVVVVGITYLSIVIGELVPKTVALNSAEKVAVAVSRPIHFVSVIFYPFVRFLALSTSL
                     FNKLIGLKPKDDVVSEMELRAMLKTASKEGVIEREENIIHEQVFYFSDKRARHLMTHR
                     TDVEWVDISKEKEEVIADLLQTKHGRVLVCNKTLDEFVGIVTMKELLSELYRKGEIDI
                     HKLVHDPLIIPSTLRARKVLEQFRQQHKFFAVVVDEYGSLDGIITLHDIIENLVGSIP
                     DENEMEEPDVFMRNENSALINGEAPIEVLTNFIDDFIIDFEEIDYSTVAGFVLSNINK
                     IPQVGDVFTYENTTFEVVDVDGNKIDKVMVTKKTKVSHEYL"
     gene            4741..5814
                     /locus_tag="PLI30_05450"
     CDS             4741..5814
                     /locus_tag="PLI30_05450"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019540801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase family protein"
                     /protein_id="HOI79035.1"
                     /translation="MDNMYSKSGLLSGAFAKELDGEQTGLYVLTNKLGSEITITNYGA
                     KIVSLMVPDKSGTLTDIVLGHSSIEEYLTSEEPYFGAVCGRTANRIACGKFTLGDKEY
                     KLAVNNGPNSLHGGLKGFNAVVWNVVNVSENSIELHYLSVDGEEGFPGNLDVYVTYTL
                     TNDNALDIFYKAVTDQTTILNLTNHSYFNLSGEGDPYIGDHVLTLNADAYLPTDATAI
                     PYGAAEPVKDTPMDFTTPRTIGERINDDFEQLRFGKGYDHTFVLNKQSDNEYSYVGVC
                     ESPKTGIKMEMFTTEPGVQVYTGNWMTGGFSAKKGHRYPERSAVCFETQHFPDSINKP
                     EYPSVVLKPGEIFNSRTTYKFST"
     gene            5862..7169
                     /locus_tag="PLI30_05455"
     CDS             5862..7169
                     /locus_tag="PLI30_05455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019540802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOI79036.1"
                     /translation="MNTTTNQKSNRIVPIVMMIALFGMIAFVTNLAAPMGQVLKEQFG
                     ASNFQGLLGNMANFIAYAVMGIPGGILLQRVGYKKTALIAIVIGFVGVFTQFLSGHAP
                     QTAAFGVYLLGAFIAGFSMCLLNIVVNPMLNTLGGGGNKGNQLIQVGGSFNSVMATFT
                     PAFVGILIGETAKANIKDVFPVMYIAMGVFALVFFVLLVVNIPEPHATKTKEPLKNLM
                     SGAMKFRHFILGAIAIFVYVGVEVGTPGILILWLSDTPVGKTTAGFVAGTYWFLMLVG
                     RLLGASLGSKVSSKTMLATVSSIGIILVLAAILSPTTTQVAIPALQTSATGALSFGMA
                     QVPINAMFIVIIGICTSIMWGSIFNLSVEGLGRFTPAASGIFMTLVCGGGILPGIQGA
                     VADLGNDYLVSYWVIFIGLAYLLYYALIGSKNVNKDISTTDVE"
     gene            7237..>7908
                     /locus_tag="PLI30_05460"
     CDS             7237..>7908
                     /locus_tag="PLI30_05460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019540803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase family protein"
                     /protein_id="HOI79037.1"
                     /translation="MTKEQVLKIYQEKFGNNTPEVYASPGRVNLIGEHTDYNGSFVFP
                     GAIDKGMIAAIRLNGTEKVRAFAIDLNESSEFGLNEEDLPQEGWAKYIFGVCREIIKR
                     GGAVAGFDTVFAGDVPLGAGMSSSAALESTYAYALNDMFNLGIDTFELARIGQSTEHN
                     YVGVKCGIMDQFASLFGKKGHLIRLDTKTMEYQYFPFDPEGYKLVLLDTVVKHELASS
                     AYNKRR"
BASE COUNT         2324 a         1551 c         1876 g         2157 t
ORIGIN      
        1 cgcaaggaat ttgacaaaaa agagatacac tacctggaaa ccattcttgc cgacggttcc
       61 aaatcggctg tgtttacggt taacgacata cccagaacgg cagagatact gcattttgcg
      121 ttgaagggac tagaagttcc ggttataaga ggagtgctga acttaagact ggacaggcag
      181 gaagacaggg aaatagtcta taatctcgtt tttaagggat taaacaagag ataatataat
      241 cagagagata aaataaaaaa ataaacacat gaaattatta gaaggaaaaa cagcgcttat
      301 cactggtgca gcgcgcggta tcggaaaagc catcgcactg aaatacgctt cggaaggagc
      361 caacattgct tttaccgatt taactatcga tgaagtgggt aaagctaccg aaagcgaaat
      421 agcatcgctg ggcgtgaaat gcaaaggata tgcctccaac gctgcaaact ttgacgaaac
      481 acacaaggta gtggaagaag tcctcagcga tttcgggcgg atcgatatat tggtcaacaa
      541 tgccgggatt accaaagacg gattaatgat gcgtatgacc gagcagcaat gggatgccgt
      601 aatcaatgtg aacctgaaat cggcatttaa ttttacccat gcagtcactc cggtaatgat
      661 gcgccagaaa gcaggaagca taatcaacat gagttccgta gtgggtgtat ccggaaatgc
      721 cggacagtca aactattccg cttccaaagc agggatgatc gggctgacaa agtcgatggc
      781 aaaagaggtg ggttcaaggg gaatccgtgt aaatgccatt gctccgggtt ttataattac
      841 agatatgact gctgcgctct ctgaagaggt tcgcaacgag tgggcaaaac aaattccgtt
      901 acgccgtggt gggacacccg atgatgttgc taatacggct gtctatcttg catcggaact
      961 ctcttcgtac gtgagcggac aggtgattca cgtgtgtgga ggaatgaaca tgtaaagaaa
     1021 atgaccgttt tatacgaaga caaccatctg gttgtcgtta acaaggctcc gggagagatt
     1081 gtgcaaggcg ataaaaccgg ggacaaacca ctttctgaag aggtgaaaga gtacctgaag
     1141 gtgaagtata acaagcccgg aaatgtattc tgcggggtga cacaccgcct ggatcggccg
     1201 acgagtggaa tagtggtttt tgccaaaaca agcaaagcgt tgtcccggtt gaatgcgatg
     1261 ttcaaaaaca atgagatcga caaaacctat tgggcagttg tgaaaaaact tccggagaaa
     1321 accgaagcca cgctgacgca ttacctcatt aaaaatgagc gaaccaataa atcaacggca
     1381 tatgatgtgg agaaacccaa cacgaagaag tcggtattgc actacaaggt gattggacaa
     1441 tcgcaaaact acaatttact ggaggttgat ctggaaacag gccggcatca tcagatccgt
     1501 tgtcagttat caaaaattgg aagtccgata aaaggagatc tgaaatacgg tgccgaacgg
     1561 tcgaatccgg atgggagcat cagtcttcat gcacggaaaa tagcatttat tcatccggtc
     1621 tcgaaagaaa aaatagaact agtggcgcca acgcctgatg atgtgttgtg gaatgcgttg
     1681 atcaatacaa acgttcactg aaacaggctt tgttgagtgt tctcaaaaat tttccgatct
     1741 acgccttttt ttctcagata gctgtttttt gttgtcgagc cttttctgaa tggagctcag
     1801 ggtacgtttc gtggccacgc gttttttttc ttcgcgacat gctttcttca ggaggttctc
     1861 taattttaca attaccgctt tcttgttcat tgcctgggaa cgttctgtgt cgctgctgat
     1921 ctgcaagatt ccttcctggc tgatacggtt tctcaacttc tgttgtatcc gctgcttttg
     1981 ttcggtcgac aaaatctgtg attgaccgat attcaaagac aacgtaactt tggtctcgac
     2041 cttgtttaca tgctgtccac cacttccact gctgcgtaca gtcgaaaaag tacactcgtt
     2101 cattaattta tcaatgtccg gctccgtcat atcgtaaaaa tacgttcttt agaacaaaga
     2161 taagccatta gggcgggaaa aagaaatcga catttgttaa taatcttttc cgtatatgct
     2221 gtttttcgtc aatctttttc cttaaatttg tgcatcagaa cagtttttta aattagataa
     2281 atcatcaatg aaaccaacat tatttgtatt ggctgccggg atgggcagcc gttacggcgg
     2341 cttaaaacag ttagacggcg tagggccgag cggtgaaacc attatggatt attccatttt
     2401 tgatgctgta aatgccggat tcgggaaact cgtatttgtt attcgtcagt cgtttgaaga
     2461 agatttcagg gagaagatcg ttaaaaagta cgaaaagaaa atccccgtag aacttgtttt
     2521 tcaggaactg gacgccctcc ccgaaggatt tgtcgttccc gagggacgtg taaaaccatg
     2581 gggtaccaat cacgcggtga tgatgggtaa agaagtgatt caggagccgt ttgccgtaat
     2641 taatgccgat gatttctacg gccgtgaaag cttcggcgtg ctggccgatt acctgaagac
     2701 attggaaaat tcggagaaca agtacggtat gattggttac cgggtaggaa acacactttc
     2761 ggaaagcgga acggtagccc gtggggtttg cgaaactgac gaaaacggaa acctgacggg
     2821 agttgttgaa cgtactcagg taaagcgtat agatggtgct gtctgctacc gggaagataa
     2881 cggaaattgg atagcaattg acgataatac accggtatct atgaatatgt ggggcttcac
     2941 ccctgattat ttcaaccatt cggatgatta ttttgtgcat ttcttaaaag aaaacgcaga
     3001 gaacacaaaa gcggaatact ttatcccatt actggttaac caccttatcg tgaatggagt
     3061 ctctaccgtg aaggtgctgg acacaccttc caaatggttt ggtgtgactt atgccgaaga
     3121 tcgtcccggg gtagttaaaa aattgcaaga actgtcagac gagaaagttt atccgtcgcc
     3181 attgtggtag acaaaatgat tgatgtattc atcatattct ttctgattat cctcaacggt
     3241 ttttttgcca tgtccgaaat tgctgtggta tcggcaaaaa agaccaaact cgaaacggaa
     3301 cggaaaaaag gaaacaaagg agcggaaaaa gcattgaaat tgcagtccga tcccgatagt
     3361 tttttgtctt cagtgcaagt gggaatcacc ctcatcggaa tcgtaaacgg agcctatgga
     3421 ggagctacac tctctgttta cctgatacct tttttcgatc ggtttgcctg gagtgcgcca
     3481 tacgcggaaa caatatccat ggtggttgta gtagtgggta taacctattt gtcaatcgtt
     3541 atcggtgaac tggtacccaa gaccgttgca ctgaacagtg ccgagaaagt ggctgtggcc
     3601 gtctccaggc ccatccattt tgtcagcgtc atcttttatc cgtttgttcg gtttctggct
     3661 ctttccacca gtttgttcaa caaacttatc gggttgaagc ccaaggatga tgtggtttcg
     3721 gaaatggagc ttcgggccat gctgaaaacg gcatcaaaag aaggagttat agaaagggaa
     3781 gagaacatca ttcatgagca ggttttctat ttttccgata aacgtgcacg tcatttaatg
     3841 actcacagaa cagatgtgga gtgggtagat atttcgaaag aaaaagaaga agtaattgcc
     3901 gatctgttgc aaaccaagca cggaagggta cttgtttgca acaagacact ggatgagttt
     3961 gtgggtattg taaccatgaa ggaattgctt tcggaattat acaggaaagg agagatcgat
     4021 atccataaac tggttcatga ccccctgata ataccttcta ccctccgcgc aagaaaggta
     4081 ctggagcagt ttcgccaaca gcacaagttt ttcgctgttg tagtggacga atacggaagc
     4141 ctggatggaa ttattacgtt gcacgacatt attgaaaatc tggtgggttc aatccctgac
     4201 gagaatgaaa tggaggaacc cgatgttttt atgcgcaacg agaactccgc tttaatcaac
     4261 ggtgaggcac ccatagaggt gttgaccaat tttattgacg attttattat cgacttcgaa
     4321 gagatcgatt attctacggt tgcaggattt gtattgtcca acattaataa aattccgcaa
     4381 gtgggggatg tattcacgta tgaaaataca acgtttgagg ttgtggatgt agacggcaat
     4441 aaaatcgaca aagtaatggt tacaaaaaaa acgaaagtaa gccacgaata tttgtgattt
     4501 ttcttttgtt tctcatagac aaaaattatg cctgatttat ataattaata ggcaaaaaat
     4561 caggtgatta taaattttta acggaagtat attcctcaaa aaaaggattt tatgactaaa
     4621 aatatgtttt ataacacaat tttaaatttg ctgaataaat taaaaatgtg ttatttttac
     4681 acgaattttc aaagaaagat ccaatcttca tcaccatctt atttttaaaa cctcaaaaac
     4741 atggataata tgtatagcaa gtccggtttg ctatcaggcg cgttcgcaaa agagctcgat
     4801 ggagagcaaa ccggcttata cgtcctaacc aataaactag gaagcgaaat taccatcaca
     4861 aattacggtg caaaaattgt ttcgttaatg gttcccgata aaagcggaac ccttacggat
     4921 attgtattgg gtcattcctc catcgaagaa tacctgacct cggaagagcc ctacttcggg
     4981 gctgtttgtg gcaggacagc caaccggatt gcctgcggta agttcacgtt gggagataaa
     5041 gagtacaaac ttgccgttaa caacgggccc aattctcttc acggtggcct aaaagggttt
     5101 aatgctgttg tctggaacgt ggtaaatgtt tcggaaaaca gtatcgaact acactactta
     5161 tcggtagatg gagaggaagg tttcccagga aatctggatg tatacgttac ttacacgctt
     5221 acaaacgata atgccctcga tattttttac aaagcggtga ctgatcaaac cacgatcctt
     5281 aacctcacga accattccta tttcaatctt tcgggtgagg gagatcccta tatcggcgat
     5341 cacgtactga cgttgaatgc agatgcctat ctgcctaccg atgcaacagc cattccgtac
     5401 ggagctgcgg agcctgtgaa ggatacccca atggatttta caactcctcg caccattggg
     5461 gaacggatta acgacgattt tgaacaactt cgttttggaa aaggatacga tcacactttt
     5521 gtgttgaaca aacaaagcga taatgaatat tcgtacgttg gcgtttgtga atcgcccaaa
     5581 accggaatta aaatggaaat gtttactaca gagcctggag tacaggtgta tacggggaac
     5641 tggatgacgg gcggattttc tgcaaagaaa ggacatcgtt accccgagcg ttctgcggta
     5701 tgttttgaaa cgcagcactt ccccgatagt ataaataaac ccgaatatcc ttcggtagtt
     5761 ttaaaacccg gtgaaatatt caattcaagg acaacgtaca aattttccac ataacagaca
     5821 atgctcaaaa taaatcaaca tttttattaa tatcaaaaag tatgaataca acaactaatc
     5881 agaaaagtaa ccggatagtg cccatcgtca tgatgatcgc actgtttgga atgatcgcat
     5941 tcgtgaccaa cctggcggcg ccgatgggac aagtattaaa agaacaattt ggagcctcca
     6001 atttccaggg attactggga aatatggcga acttcatcgc gtatgccgtt atgggaattc
     6061 cgggagggat actcctgcag cgcgtgggct ataaaaaaac agctctcatt gccattgtaa
     6121 tcggttttgt tggagttttc acccaattcc tttccggaca tgctcctcaa acggccgctt
     6181 tcggtgttta tttgctcgga gcttttatcg ctggtttttc catgtgtttg ttaaatattg
     6241 tggttaaccc tatgttgaat acattgggag gcgggggaaa caaaggaaac cagcttattc
     6301 aggttggagg atcgtttaac tccgtaatgg caacttttac tccggctttt gtaggaatct
     6361 taatcggtga aacggctaag gcaaacataa aagacgtatt tccggtaatg tatatcgcga
     6421 tgggtgtttt tgcccttgtt ttctttgtgc tgttggtcgt aaacattcct gaacctcatg
     6481 ctacgaaaac caaagaacca ctaaagaacc tgatgagcgg cgcaatgaaa ttcaggcatt
     6541 tcattttagg tgccatcgca atttttgttt atgtgggtgt tgaagtgggt acgcccggta
     6601 tcttgatttt atggttatcc gatacaccgg ttggaaaaac aacggcaggg ttcgttgctg
     6661 ggacgtattg gtttttaatg ttagtgggca ggttgctggg agcatctctg ggcagtaaag
     6721 tatccagcaa gaccatgctt gcgaccgttt cttcaattgg aataattctt gtgttggcag
     6781 cgatcttgtc acccaccact acgcaggtgg cgattccggc acttcaaact tctgccaccg
     6841 gcgccctttc ctttggaatg gcacaagtac ccatcaacgc catgtttatc gttataatcg
     6901 gtatttgtac atccattatg tggggcagta tttttaatct ttcggtagaa gggttgggca
     6961 ggtttacgcc ggctgcttcg ggaatattta tgaccttggt ttgcggagga ggaattcttc
     7021 ctggtattca aggtgctgtg gctgatttag gcaacgatta cctggtgagc tattgggtaa
     7081 tctttattgg gttggcatac ctgctatact atgcgttgat cggatccaaa aacgtaaaca
     7141 aggatatttc taccacagat gtggaataac tccctgtgaa aacagcctct aacattaagt
     7201 tttcacagat gcgcaaatga aataattata aaaaatatga ctaaagaaca ggttttaaaa
     7261 atttatcaag aaaaatttgg taacaataca cctgaagttt acgcgtctcc cggacgcgta
     7321 aaccttatag gtgagcatac cgattacaac ggaagttttg tttttcccgg agctatcgat
     7381 aaaggaatga tagccgctat acggttaaac ggaacggaaa aagtccgtgc ttttgccata
     7441 gatttaaacg aaagttcgga gtttgggctc aatgaagagg atctacccca ggaaggctgg
     7501 gcaaaataca tattcggggt atgccgtgag attattaaac gcggtggagc cgtagcaggt
     7561 ttcgatactg tatttgcagg agatgttccg ttaggagcag ggatgtcgtc atctgctgcc
     7621 ctggaaagta cttatgcata cgcactgaac gacatgttca atctggggat tgataccttc
     7681 gaactggcac gtatcggaca atctaccgag cataactacg taggtgtgaa atgtggcatt
     7741 atggatcagt ttgcatcgct ttttggcaaa aaggggcatt tgataaggct cgacacaaaa
     7801 acgatggaat accagtactt cccgtttgat cccgaagggt ataaattggt gttgctcgat
     7861 accgttgtga aacacgaatt ggcttcatcg gcgtataaca aacgccgg
//
LOCUS       DAOSDR010000102         7861 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrimonas sp. isolate AMR_MDS_1826
            NODE_3404_length_7861_cov_4.016270, whole genome shotgun sequence.
ACCESSION   DAOSDR010000102 DAOSDR010000000
VERSION     DAOSDR010000102.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863355
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrimonas sp. (wastewater metagenome)
  ORGANISM  Petrimonas sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Dysgonomonadaceae; Petrimonas.
REFERENCE   1  (bases 1 to 7861)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7861)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:10:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,720
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 35
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7861
                     /organism="Petrimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3404_length_7861_cov_4.016270"
                     /isolate="AMR_MDS_1826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2023866"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..797)
                     /locus_tag="PLI30_05465"
     CDS             complement(<1..797)
                     /locus_tag="PLI30_05465"
                     /EC_number="3.5.4.4"
                     /inference="COORDINATES: protein motif:HMM:NF006850.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000034 - adenine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006146 - adenine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HOI79038.1"
                     /translation="MDENLKRYIELIPKAELHVHIEGTFEPELIFEIAQRNNVPLKYN
                     SVEELRKAYNFNNLQDFLDIYYGAASVLLYEQDFYDLTASYLRKAHADNVVHTEIFFD
                     PQTHTTRGVSFATVINGITRAMGDAKTEWGISTKLIMCFLRHLSEKSAEETLESALLH
                     KEKIIAVGLDSSENRHPPGKFFHVFNRVREEGFFVVAHAGEEGPAEYVWEAIDLLEAE
                     RIDHGVSSVDDPDLMEELREREIPLTVCPLSNLKLRVVEDLKNVPVRS"
     gene            944..3187
                     /locus_tag="PLI30_05470"
     CDS             944..3187
                     /locus_tag="PLI30_05470"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008141153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH92 family glycosyl hydrolase"
                     /protein_id="HOI79039.1"
                     /translation="MRSGLFFILFLVISSNFFGQDLTGFVNPFIGTTNYGTTNPGAVL
                     PNGMMSVAPFNVMGSDENKFDKDKQWWSTPYSYENKFFTGFSHVNLSGVGCPEAGSLL
                     LMPTSGELNVNYEEYGSGYAGEQASPGYYTNRLTKYGILTEVSATTRTSIARFTFPKG
                     HANILLNLGEGLTNESGAWMRRISNTEIEGMKVLGTFCYNPQAVFPIYFVMRVSKPPV
                     TAGYWKKQREMTAEAAWDSTAGTRKIYTRYGRDIAGDDIGAYFSYDVKAGETIEVQIG
                     VSFVSTANARENLEAEQNGFQFDKVRTAARESWEKELARVEIEGGTADQKVVFYTALY
                     HALIHPNLFNDVNGQYPAMESDKILTSGAGRYTVFSLWDTYRNVHQMLSLLYPEKQLD
                     MVRSMVDMYKESGWLPKWELYGRETLTMEGDPAIPVIVDSWMKGLRDFDVETAYEAMY
                     KSATTKGKDNLLRPDNDDYLRLGYVPLREKYDNSVSHALEYYIADNALSKFAAALGKK
                     EDAARFRAQSLNYSRYYSPEYGVFRPLLPDGTFLSPFNPKQGENFEDVPGFHEGSSWN
                     YSFMVPHDVPGLIKLHGGNRKFTNKLQEVFDDEHYDPTNEPNIGYPYLFSYVKGEEWR
                     TQQLTQQLLTRYFKNSPNGLPGNDDTGTMSAWAVFSMMGFYPDNATEPFYTFTTPIFD
                     RITLRLNPDYYDNKVLEMEIVRPGKNARFIDKIVIDGKKFNGYRISHEQLSKAQKIVF
                     YLKERNH"
     gene            complement(3195..4502)
                     /locus_tag="PLI30_05475"
     CDS             complement(3195..4502)
                     /locus_tag="PLI30_05475"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HOI79040.1"
                     /translation="MKYEYSKGYEIVRFFERITLNLHYRKIVYKGTENINEDHPVIFA
                     SNHRNAVIDPFLLLNACKKEPVFLARADVFRKPTVAAIMKWLHIMPVYRIRDGVENLG
                     NNNESFRLSGELLKKRIPMALYPEGRHNPKMSLLPVQKAISRIVLPIEASEDFTLNSE
                     VIPVGVYYPDIFGFLSDAYVVFGKPIQVADYRKQYEENPNMAANSLRHDLENSMKRLI
                     VNIWNDAFYDEYVWAIDWNAPQLVRGKYAGKQEDYLLASQEVVDTLDEMFHRDRPSFD
                     THIENFRRAESILKKHRLTSRDNVLKPASTVLIVLHLLLLIVSLPLAVFGFVNGIFPI
                     IGYRKLLGLFKDDQFIPTVRVVSGMFIVPAFVVIQSLIVGLVFNGWWAFAYSFLMPAT
                     FYFACWWRKWAKSLARKWCVNRFVKKFPDSWRELTELIKINHS"
     gene            complement(4499..5467)
                     /locus_tag="PLI30_05480"
     CDS             complement(4499..5467)
                     /locus_tag="PLI30_05480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019538090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bile acid:sodium symporter family protein"
                     /protein_id="HOI79041.1"
                     /translation="MQHLPLLSLFGNLEQLNTVQLNFNQNSLNMMNIAIAFIMFGVAL
                     GIRPKHFRDIALNPKPAIVGVISQYILLPALTYLLVLVIKPSVPVAMGMILVASCPGG
                     NVSNMISALSKSNVALSVSLTATTTVLSLFMTPLNFAYWGGLYASKSPLLVPITIDPV
                     EMFRTVFIILGIPVIMGMIVGMKFPVFVKKVEKHVQIASVLFFIAFLAGALAGNFSLF
                     LKYIHLVFLIVLLHNGIAFLGGYLFPRTLKINKLNSRTISIETGIQNSGLGLALIFNP
                     RVFPPELELGGMAFIAAWWGIWHIVAGLMLAFYWRRESIKHIAVTS"
     gene            5680..6384
                     /locus_tag="PLI30_05485"
     CDS             5680..6384
                     /locus_tag="PLI30_05485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI79042.1"
                     /translation="MKEEKLVRCRPCGYVMKESELGDVCPACGLPRDVFEPYREKVSS
                     GRLRFLALDIHPIAIHLSQTFVALVPFLIIFHYLFPDFEPTIIHSVIAFSVYLFPLTL
                     ILSALSGYADGLVRFKTINTPLLLKKIILSVIVIALAVIQAIVFRRDIYPWYFLLLSL
                     GSLATAVQLGMLGKHLINVILPGTLVLRGVKKQASSAEPVKSPKMSPEEIARRVQEKQ
                     AAKARAQKENGTNNTE"
     gene            6430..7443
                     /locus_tag="PLI30_05490"
     CDS             6430..7443
                     /locus_tag="PLI30_05490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019540082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HOI79043.1"
                     /translation="MKDKEIFTERLSRRGFLGAAAAATAASVIPFSTSYGSTQSSTLG
                     KGIPNSKFSGVQVGAITYSFRDLTGGLEAVLKACVDAGLSSVELMGTGVEDYLGAPKN
                     PLQRPPGAPRQLSDAERKTLDQYNRDLKEWRKVNGTLDKYAQLRKKFNDAGVKIHIYK
                     WVAGNTEEELDYSFKVAKTLGAIGISAELNDENARLMGPVAARNGMYAILHNHYQFGE
                     PGFDVDGLLAYSPANMLNFDIGHYLGSTGLNPADFIRKYYNRIASIHVKDKTRATNAT
                     QKNTNQVFGQGETPIAEVLRLIQKEKWPIYCDIELEYPVVPWSTSVKEVRTCMEYCRQ
                     ILI"
     gene            7458..>7861
                     /locus_tag="PLI30_05495"
     CDS             7458..>7861
                     /locus_tag="PLI30_05495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008671826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOI79044.1"
                     /translation="MSAPLAERLRPRSLDEFIGQTHLVGKGAVLRKMIDSGRIPSIIF
                     WGPPGVGKTTLANIIANTLHAPFYKLSAINSGVKDVRDVIEKAKNNRFFEAAAPILFI
                     DEIHRLSKSQQDSLLHAVETGIITLIGATTENP"
BASE COUNT         2117 a         1791 c         1890 g         2063 t
ORIGIN      
        1 gacctgaccg gaacattttt caggtcttcc actaccctta gtttgaggtt tgataatggg
       61 catacggtca gggggatttc ccgttcacgc agttcttcca ttaaatcagg atcatccaca
      121 gagctgaccc cgtgatcgat ccgttctgcc tccagcaggt cgatggcctc ccagacatat
      181 tctgcaggac cttcttctcc ggcatgggca accacaaaaa agccttcctc gcgtacccgg
      241 ttgaaaacat ggaaaaactt tcccggcgga tgtctgttct ccgaagaatc gagccctacg
      301 gcaatgattt tttctttatg caacaaggcg gattccaggg tctcttcggc ggatttttcg
      361 cttaaatggc gcaggaaaca catgatcagt ttcgttgaaa tcccccattc ggttttggcg
      421 tcacccattg cccgggtaat tccgttgata accgttgcaa agcttacgcc gcgggtggta
      481 tgcgtttgcg gatcgaaaaa gatttcggta tggacgacat tgtcggcatg tgccttccgc
      541 aagtagcttg cggtgaggtc gtaaaaatcc tgttcgtaca aaagtacgct ggctgctccg
      601 tagtagatat ccagaaaatc ttgcaggtta ttgaaattgt atgctttacg caactcttcc
      661 acggagttgt actttagggg gacattattc ctttgagcga tttcgaaaat cagctcaggt
      721 tcgaacgtac cttcgatatg tacgtgcagt tctgccttgg gaattaactc aatgtatctt
      781 tttagatttt cgtccatact ctttgttgtt gctgattatt tttgcaaaaa tacacgaagc
      841 tttcggtttc aaaaattatt tgcaaaagaa tttaggttgt gttggacgat tatggttatt
      901 tttgcacgaa atataggtag aagttgaatc aatcatttat cgtatgcgat ccggactgtt
      961 ttttattctt tttcttgtga tttcttcgaa ttttttcggt caagacctta ccggttttgt
     1021 caaccccttt atcggtacga ccaactacgg caccaccaat cccggggcgg tactacccaa
     1081 cggaatgatg tcggtggccc cgtttaacgt gatggggtcg gatgaaaaca agttcgataa
     1141 ggacaagcaa tggtggtcga caccctactc gtacgaaaat aagttcttta ccggattctc
     1201 gcacgttaac ctgagcggag tggggtgccc cgaggccggc tctcttttgc tgatgcccac
     1261 atcgggagaa ttgaatgtca attacgagga gtatggcagt gggtatgccg gtgagcaggc
     1321 gtcgcccggc tattatacca accggttgac caaatacggc atattaaccg aagtttcggc
     1381 cactacacgc acgagtatcg cccgttttac ctttccgaaa gggcacgcaa atatattgct
     1441 gaacctgggg gaagggctta ccaacgaaag tggtgcctgg atgcgtcgca tcagtaatac
     1501 cgaaattgag gggatgaaag tgctggggac tttttgttac aaccctcagg cggtttttcc
     1561 catttatttt gtgatgcgtg tcagcaaacc accggtgact gccggatatt ggaagaaaca
     1621 acgcgaaatg actgcagagg ctgcttggga cagcacggca ggcacacgga aaatttacac
     1681 ccgttatggg cgggatattg ccggagacga tatcggagcc tacttttctt acgatgttaa
     1741 agcgggtgaa accatcgaag tacagatcgg ggtttccttc gtaagcactg ccaatgcccg
     1801 cgaaaacctg gaggccgagc aaaacggctt tcagtttgat aaggtcagga ctgctgcccg
     1861 cgaaagctgg gagaaagagc ttgcccgtgt agagattgaa ggaggtaccg ccgaccagaa
     1921 agtggttttc tataccgcac tttatcatgc gctcatccat cccaacctgt ttaatgacgt
     1981 gaacggtcag tatccggcaa tggaaagtga taagatcctt acctccggag cagggcgata
     2041 cacggttttt tcgctttggg acacgtacag gaatgtgcat cagatgcttt cactgcttta
     2101 tcccgaaaaa caattggaca tggtccgttc gatggtggat atgtacaaag aaagcggatg
     2161 gctgcctaaa tgggaattgt atggccgcga gactcttacg atggagggag acccggctat
     2221 tccggttatc gtggacagtt ggatgaaagg gttgcgcgac ttcgacgtgg aaacggctta
     2281 cgaagccatg tataaatcgg ccactacgaa aggtaaagac aaccttcttc gcccggataa
     2341 cgatgattac cttagactgg gttatgtgcc gcttcgtgaa aaatacgaca attccgtttc
     2401 tcacgcgctc gaatattaca tcgccgataa cgccttgtca aaatttgctg ctgccctagg
     2461 aaaaaaagaa gatgctgccc gcttccgcgc ccagtcgctg aattattccc gttattactc
     2521 gcccgaatac ggggtgttcc gcccgctatt gcccgacgga actttcctgt cgcctttcaa
     2581 tcccaaacag ggcgaaaact ttgaagacgt acctggattt cacgaaggca gttcatggaa
     2641 ttattcgttt atggtgccgc acgatgtgcc gggactgatt aaactgcacg gcggtaacag
     2701 aaaatttaca aacaagcttc aggaggtgtt tgacgatgag cattacgacc ccaccaacga
     2761 gccgaatatc ggttatccct atcttttcag ttatgtgaaa ggggaggagt ggcgtacgca
     2821 acaacttacg cagcagcttt tgacccggta ttttaagaat tctcctaacg gacttccggg
     2881 aaatgatgat acgggcacca tgtctgcctg ggccgtattc tcgatgatgg gtttttatcc
     2941 cgacaacgcc acagagcctt tttacacttt tacaactccg attttcgata gaataacgtt
     3001 gcggttaaat ccggactact acgataacaa ggttttggag atggaaattg ttcgccccgg
     3061 aaaaaatgcc cggtttatcg ataaaattgt gatcgacgga aaaaaattca acggctaccg
     3121 tatttcacac gaacagctgt cgaaagccca aaaaatagtt ttttacctga aagaaaggaa
     3181 tcattaacgt tgagtcagct gtggttgatt ttaatcagct ccgttaactc tctccaactg
     3241 tcggggaact tcttcacaaa tcggttaacg caccattttc tggcaagaga ttttgcccat
     3301 ttacgccacc agcatgcaaa atagaacgtt gcaggcatta gaaaagaata ggcgaacgcc
     3361 caccatccgt tgaaaaccag gccaacgatt agggattgaa taacaacgaa agcggggacg
     3421 ataaacatac cggaaacgac acggaccgtc ggtataaact gatcgtcttt aaaaagcccg
     3481 agtagtttcc tgtagccaat tatgggaaag attccgttga caaacccgaa aacggccagg
     3541 ggcagcgaaa cgatcaggag caacaggtgc aagacgatta aaaccgttga ggccggtttt
     3601 aagacattgt ccctggatgt taaccggtgt tttttgagta tgctctctgc cctgcggaaa
     3661 ttttcaatgt gcgtatcgaa ggagggcctg tcccggtgga acatctcatc cagcgtgtcg
     3721 acaacctcct gcgaagccag cagataatct tcttgcttcc cggcatattt tccccttacc
     3781 agttggggtg cgttccaatc gatggcccaa acgtattcat cgtaaaacgc atcgttccat
     3841 atgttcacga tcagtctctt catgctgttt tccagatcgt gcctcaaaga gttggctgcc
     3901 atgttggggt tttcttcgta ttgtttgcgg taatccgcca cttggatggg tttcccgaaa
     3961 actacgtacg catcggaaag gaatccgaaa atatccgggt aatatacccc tacgggaata
     4021 acttcgctgt tcaaggtgaa atcctcgctt gcttctatgg gcaatacaat tcgggaaatg
     4081 gccttctgaa caggcagtaa tgacattttg ggattatgcc tcccttctgg atatagcgcc
     4141 atcggaattc tctttttcag cagctctccg ctcaaccgaa acgattcgtt gttgttcccc
     4201 aggttttcca ccccatcccg gatgcgatat accggcataa tatgaagcca cttcatgata
     4261 gcggcaacgg taggtttcct gaatacatcc gcacgggcca ggaaaaccgg ttccttttta
     4321 caagcgttca acaacaaaaa aggatcgata acggcgttgc ggtggttcga tgcaaaaatt
     4381 accggatgat cttcgttgat gttttcagtc cccttgtata caattttcct gtaatgcagg
     4441 ttgagggtga tccgttcgaa aaaacggaca atttcgtatc ctttactgta ttcgtacttc
     4501 atgatgttac agcaatgtgt ttaatggatt ctctccgcca gtaaaaagcc agcatcagac
     4561 cggcaacaat gtgccagatt ccccaccagg cagcaataaa tgccattccc ccaagctcca
     4621 gttccggagg aaaaacccgg ggattaaaaa ttaaggccag tcccagcccc gagttctgta
     4681 ttcccgtttc gatagagata gtccgggagt taagtttatt tatcttgagg gttctgggga
     4741 acaggtatcc gcctaagaag gcgatcccat tatgcagtaa aacaatcaaa aaaaccaggt
     4801 ggatgtattt cagaaaaagg gagaaatttc ccgccaacgc tcctgccagg aaagcgataa
     4861 aaaataaaac cgatgcaatc tgtacgtgtt tttccacttt ttttacgaat accggaaatt
     4921 tcatcccgac gatcattccc attatcacag gtattcccag aatgatgaaa acagtgcgga
     4981 acatttccac gggatcgatg gtgattggaa ccagaagtgg ggatttgcta gcgtaaagcc
     5041 cgccccagta agcaaaattg agaggcgtca taaacaaact caatacggtg gtggttgccg
     5101 tcaggcttac agacaaagcc acattcgact tcgacaatgc cgaaatcatg tttgagacat
     5161 tcccgccggg acaggaagct accaggatca ttcccattgc taccgggacg ctcggtttga
     5221 taacgaggac cagcaggtaa gttaaagccg gcagcaggat atattgcgag ataaccccca
     5281 ctatagccgg cttgggattc aatgcgatgt ccctaaaatg tttgggtctt atgcccaacg
     5341 ccacaccaaa cataataaac gcgatggcta tattcatcat gtttaacgag ttttggttga
     5401 agttgagttg taccgtgttc agctgttcca ggtttccgaa tagtgacaaa agcggcaaat
     5461 gttgcatagg gagtacttta tgatttccag ccgcaaaggt aacttttttg aaaagaaacg
     5521 caaagctaga tttcgataaa tctgttttaa attcatttaa taaaatatcc aatcaaaatt
     5581 aattgctatt tttgcaaagg ataatcgttt cctatgtttt acgtatatta attaccaaca
     5641 tacatataaa gaggttaaaa aaattacttc cgcgaaaaca tgaaagaaga aaagttagtc
     5701 cgttgccgtc cttgcggtta tgtaatgaaa gagagcgaac tgggtgatgt ttgtcctgct
     5761 tgtggtttac cgagagatgt atttgagccc taccgggaaa aggtctcttc cggacgtttg
     5821 cggtttttag ccctggatat ccatcccata gctattcacc tttcgcagac atttgttgcc
     5881 cttgtgccgt ttctcatcat cttccattat ctcttcccgg actttgagcc gacgatcatc
     5941 cattcggtga ttgccttttc ggtatacttg tttccgctca cccttatatt gtctgcatta
     6001 tccgggtatg cagacggact tgtacgtttt aaaacgataa acaccccttt gttgttaaag
     6061 aagattattc tcagtgtcat cgtgattgca ctagccgtta ttcaggcgat agtttttagg
     6121 cgtgatattt acccctggta ctttctgctt ctttctctcg gatcgcttgc taccgccgtg
     6181 caactgggta tgttgggaaa acacctaatc aacgtaattc ttccgggcac tctggtgctc
     6241 cgcggtgtga aaaaacaagc gtcatccgct gagccggtaa aaagtccgaa aatgagtccg
     6301 gaagaaattg cccggcgggt acaggaaaag caagcagcaa aagcgcgtgc gcagaaagaa
     6361 aacggaacga acaatacaga ataattcctg agaaatacag atatcagttt atcaaataaa
     6421 aacaataaaa tgaaagacaa agagattttt acagaaaggc tttccagaag aggttttctg
     6481 ggtgctgctg ctgctgcgac ggctgcttca gtaattccgt tttctacatc gtacgggagt
     6541 actcagtcat cgactctggg aaagggtatt ccgaattcga aattcagtgg agtccaggtc
     6601 ggtgctatta cctacagttt ccgtgacttg accggtggcc ttgaagcagt tttaaaggct
     6661 tgcgttgatg caggcttaag ctccgttgag ctgatgggta caggcgtgga ggattacctt
     6721 ggagctccca aaaatccgtt gcaacgtcct cccggtgctc cgcgtcagct ttccgatgca
     6781 gaaaggaaga cgctggatca gtacaaccgc gacttgaaag aatggcgaaa agtaaacgga
     6841 acgctggata aatatgccca actccgaaaa aaattcaacg atgcgggtgt gaaaatccat
     6901 atctataaat gggtggccgg aaatacggaa gaggaactcg attattcatt caaagtggcc
     6961 aaaacacttg gggcaatagg aatcagtgct gaactgaacg atgaaaatgc ccggctgatg
     7021 gggcccgttg ccgccagaaa cgggatgtac gccattttac acaaccatta tcagtttgga
     7081 gagcccggtt ttgatgtaga cgggttgttg gcatactcac cggccaacat gctgaacttc
     7141 gatatagggc attacctggg gtcaaccggg ttgaaccctg ccgattttat tcggaaatat
     7201 tacaaccgga ttgcaagtat tcacgtaaag gataagacgc gcgcaacgaa tgccacgcaa
     7261 aagaatacta accaggtatt cgggcaggga gaaaccccca ttgccgaagt gttgcgctta
     7321 atccagaaag agaagtggcc aatttattgc gatatcgaat tggagtatcc ggttgtaccc
     7381 tggtcaactt cggtaaagga ggtaagaacc tgtatggagt attgccggca gatattgatt
     7441 tgatccgtga gaaagtgatg agtgcacctt tggcagaacg gttgcgtccg cggagcctgg
     7501 acgaatttat cgggcaaaca catttggtcg gtaaaggggc tgttttgcgc aagatgatcg
     7561 attcgggccg gatcccttcc atcattttct ggggaccgcc gggtgtcggc aaaactacgc
     7621 tggcaaacat cattgccaac acgttgcacg ctccgtttta taagttaagc gccattaatt
     7681 cgggagtaaa ggatgtgcgg gacgtgattg agaaggccaa gaacaaccgt ttttttgaag
     7741 ctgctgcccc cattctattt attgatgaga ttcaccgctt gagcaagtcg caacaggatt
     7801 ccctgttaca tgccgtagaa accggaatca ttacgctcat cggtgcaaca acggagaatc
     7861 c
//
LOCUS       DAOSDR010000103         7860 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrimonas sp. isolate AMR_MDS_1826
            NODE_3407_length_7860_cov_3.935298, whole genome shotgun sequence.
ACCESSION   DAOSDR010000103 DAOSDR010000000
VERSION     DAOSDR010000103.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863355
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrimonas sp. (wastewater metagenome)
  ORGANISM  Petrimonas sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Dysgonomonadaceae; Petrimonas.
REFERENCE   1  (bases 1 to 7860)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7860)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:10:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,720
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 35
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7860
                     /organism="Petrimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3407_length_7860_cov_3.935298"
                     /isolate="AMR_MDS_1826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2023866"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..458)
                     /locus_tag="PLI30_05500"
     CDS             complement(<1..458)
                     /locus_tag="PLI30_05500"
                     /inference="COORDINATES: protein motif:HMM:NF016060.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HOI79045.1"
                     /translation="MRRILVLTGKLIGDLIDFFYPPFKRYFSRQFFRYGVSGAANVGF
                     DWLLYFVVYNYIIQKQFVPLGFVTISPHIAALLITFPVTLFSGFLLQKYVTFTSSDLR
                     GKEQLVRYMAVVGVNLLINYFGLKLLVEVLHLYPTPSKMVVTLICTIFSY"
     gene            complement(474..1379)
                     /gene="menA"
                     /locus_tag="PLI30_05505"
     CDS             complement(474..1379)
                     /gene="menA"
                     /locus_tag="PLI30_05505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006842357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046428 - 1,4-dihydroxy-2-naphthoate
                     octaprenyltransferase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     octaprenyltransferase"
                     /protein_id="HOI79046.1"
                     /translation="MAKLKNWISAFRLRTLFLAVAGVTLGTGVALQEGKFSAITFVLA
                     LALAVSIQILSNLANDLGDYLKGTDVTGNRQGPARAVQSGKISPGEMKTAIGIAIVIV
                     AAIGLTLVLDAAESYTQPSVFVLLGIGLVCILSALFYTIGKYAYGYVGLGDFFAFLFF
                     GPVAVVGTYFLHTHSIGFQPVLPAVGLGFISTMVLNINNMRDMDNDKASGKITFALRL
                     GLKNAKIYHTLLTFGMFTCFLQYSFMFAALPGYRFLYVAVFLYQFYLLTQVHKKTGRE
                     LDPYLKLTSMSGFLLAVVFSICINI"
     gene            complement(1382..2863)
                     /gene="proS"
                     /locus_tag="PLI30_05510"
     CDS             complement(1382..2863)
                     /gene="proS"
                     /locus_tag="PLI30_05510"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010537985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HOI79047.1"
                     /translation="MAKELKELTSRSEDYSQWYLDLVIKAELAENSAVRGCMVIKPYG
                     YAIWEKMQRQLDDMFKETGHVNAYFPLFIPKSFLSREADHVEGFAKECAVVTHYRLKN
                     APDGSGVVVDPEAKLEEELIIRPTSETIIWSTYKNWIQSYRDLPLLINQWANVVRWEM
                     RTRLFLRTAEFLWQEGHTAHATEQEAVEETEKMINVYADFAEKYMALPVIKGKKSASE
                     RFAGAIDTYTIEALMQDGKALQSGTSHFLGQNFAKAFDVMFADKDGGRDYVWATSWGV
                     STRLIGALIMAHSDDNGLVLPPKLAPYQVVIVPIYKNAEQLAQIDEKVSGIVSRLKAL
                     GVSVKYDNSDNKKPGWKFSEYELKGVPVRLAMGGRDMENNTIEVARRDTLTKETVSCE
                     NIEEYVKNLLDEIQENIYKKALDYRASTTREVNSYEEFKEEIEKGGFLLCHWDGTPET
                     EELIKNETKATIRCIPLEGDKTPGKCMVTGKPSAQRVIFARAY"
     gene            complement(2968..3471)
                     /locus_tag="PLI30_05515"
     CDS             complement(2968..3471)
                     /locus_tag="PLI30_05515"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010800510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight
                     protein-tyrosine-phosphatase"
                     /protein_id="HOI79048.1"
                     /translation="MQIPPAKNQQVNVLFVCLGNICRSPAAEGILKKMVADNRLEERI
                     FVDSAGTSGWHEGELPDDRMRMHGQRRNYDFCSRSRKFVSTDFERFDYIIVMDDDNYR
                     NVKKLAKTEEDVAKIHRMIDFSSKFSHHHKVPDPYYGGASGFELVMDLLEDACGNLLD
                     KIKSNFG"
     gene            3558..4031
                     /locus_tag="PLI30_05520"
     CDS             3558..4031
                     /locus_tag="PLI30_05520"
                     /inference="COORDINATES: protein motif:HMM:NF016211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaN family protein"
                     /protein_id="HOI79049.1"
                     /translation="MNIPEERKSQPISKEITVVEAKKELEKNRSKRILFATGGSFALV
                     LAVLGVFVPGIPCTPFALLSAALFAKSSEKLYNWLLSNRILGPRIKNYQRRKGISKGG
                     KVKVIILMVTMVLVSSFLIINVPAIRIVILLSGLVGAIVVWFFVPEGRDYTEEEG"
     gene            complement(4028..5251)
                     /locus_tag="PLI30_05525"
     CDS             complement(4028..5251)
                     /locus_tag="PLI30_05525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019540629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HOI79050.1"
                     /translation="MQQKRKIINDPVFGFVNIPDEFIYELIQHPCLQRLNRIRQLGMA
                     SYVYPGAQHTRFHHTIGAMYLMDVALLNLRDKGHEITKDEYRGALAAILMHDIGHTPF
                     SHVLENTLANNVPHEEVSLLLMQQINGEKKGALQTAIDIFRDKYPKRFLHELVSGQLD
                     VDRLDYLQRDSFFTGVSEGGIGAARIMKMLDVIDDKLVVESKGIYSIENFLMSRRFMY
                     WQVYLHKTAVASEKMLTNTINRAKYLSRKGEDLFASPSLAFFLKNDITLKDFRESPEV
                     LEHFTNLDDNDIWTSLKVWKSHSDKVLSLLSHGMVNRKLFKIQITNEPHPTEKKQEFI
                     EKFTRKYGISESEAAYFVSADSLATDMYNKYDESIKILYRDGSIKDISTASDMFNIEL
                     LSKKVEKYYFAYLRD"
     gene            complement(5305..5375)
                     /locus_tag="PLI30_05530"
     tRNA            complement(5305..5375)
                     /locus_tag="PLI30_05530"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5341..5343),aa:Gln,seq:ctg)
     gene            complement(5484..>7860)
                     /locus_tag="PLI30_05535"
     CDS             complement(5484..>7860)
                     /locus_tag="PLI30_05535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019539769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AAA domain-containing protein"
                     /protein_id="HOI79051.1"
                     /translation="TLEPSFFSERYGFQGRLDLLHINKKAYEIVELKSGKLPYPAYDT
                     GKIALNHEVQTGVYRLMTESVFDVPSRRVEAAILYSSGSIPGTNLRFAAGFQQLEKEI
                     INVRNLIIANEHAIINGNNQTVAQLFQALYDTTGTAQKSATFYTQRIEQFKSVLQQCT
                     PMELSYFYRYIRFVSRELYLQKTGDVEYESPTGVASLWNSDFTERAEALDVLYGLSIE
                     SIDDSGNDMKIVFRRNHAGNDVVNFREGEICIVYPRQDEQDTVLNRQILKGALAAISR
                     EFVEVRFRNKQRNRTFFNENPLWAIEHDALDTSYNSMYKSLFDFLNAEKQQRDLLLGL
                     RAPQAPAIPENKLPYPESIIRKAMAAEDYFLIIGPPGTGKTSIFARRLVEEFYTKENG
                     SILVLAYTNRAVDELCEAINAAFGCKDENSSNYIRVGSELSCAEAYRDRLLQNISEKA
                     SNRESLRTTIRKTRIVVSTLASINGKPELFSLKKFDVAIIDEASQILEPQIIGLLPRF
                     DKFIMIGDHHQLPAIVLQKKQVAAIHEPELTGAGITSCAHSLFERLLRNCVRNNWTHA
                     YTQLTIQGRMHEQIAAFPSTHFYPQALLSAKEWQAQPWRSSFPETGNVFLQHVSKSRI
                     AFFSTEQLPPQSTSSKTNTPEAEIVAEVIRSVATVYAHDRRNFDPRNIGIIAPYRNQI
                     ALIKHQLEKAGIPAYENIMVDTVERFQGSQRDVIIISFCVNKPYQLHFLCNLNDEGTV
                     DRKLNVSLTRARQQLFMVGNRQILKKHPVYATLMEFLKEKTVVLEATGINP"
BASE COUNT         1922 a         1891 c         1945 g         2102 t
ORIGIN      
        1 aaatagctga agatcgtaca aatcagcgtc accaccattt tcgagggtgt ggggtagagg
       61 tgcagcacct ccaccagtag ctttaatccg aaataattga tcagcaggtt cactcccaca
      121 acagccatat agcggacgag ctgttccttg ccacgcaaat cggaagaggt gaatgtgacg
      181 tatttttgaa gcagaaatcc cgagaacagg gtgaccggaa acgtgatgag cagagccgcg
      241 atgtgcgggc tgatggtcac aaacccgagc ggaacgaact gcttttgtat gatgtagtta
      301 tagacaacga aatagagcag ccagtcgaaa cctacattgg cggcaccgct tacgccatag
      361 cggaaaaact gtcgactgaa atagcgcttg aacggcggat aaaagaagtc aatgagatcg
      421 ccgataagtt ttcccgtaag cactaaaatt cttctcattc cttttcagac ggtttaaata
      481 ttgatgcaga tgctgaaaac aacggctaac agaaaacctg acatcgaggt aagcttaagg
      541 tagggatcca gctcgcggcc tgttttcttg tgaacttgcg ttaagaggta gaactggtaa
      601 agaaaaacag ccacatagag aaaccggtat ccgggcaatg ctgcaaacat aaaactgtac
      661 tgcaaaaagc aggtaaacat tccaaaagtg agcaaggtat ggtagatctt ggcatttttt
      721 aagcctaacc tcaatgcaaa ggtgattttc cccgatgcct tgtcgttatc catgtcgcgc
      781 atgttgttga tgttgagtac catcgtgctt atgaagccca agcctacagc tggcaagaca
      841 ggctgaaacc cgatggagtg ggtgtgcagg aaataggttc cgacaaccgc tacagggcca
      901 aaaaacaaaa aagcaaaaaa atcacccaac cccacgtagc cgtaagcata cttgccgatg
      961 gtgtagaaca gggccgacag gatacagacc aacccgatgc ccagcagtac gaaaacggac
     1021 ggttgggtat aactctccgc agcgtctaaa accagcgtaa gcccgattgc agcaacgata
     1081 acgatagcga tcccgatggc tgttttcatc tccccgggag aaatttttcc gctttgcacc
     1141 gcacgggcag ggccctgtcg gttgccggta acatcggtac ctttcaggta gtcgcccaag
     1201 tcgtttgcca ggttcgacaa gatctggatg gatacagcca gtgccagtgc caatacaaaa
     1261 gtgattgcag agaattttcc ttcctgtaag gcaactcccg tacccagggt aactccggcg
     1321 acagccagaa agagtgtgcg aagcctgaaa gccgagatcc agttttttaa cttcgccatc
     1381 tttaatatgc ccttgcgaaa atcacccgtt gtgccgacgg tttaccggta accatgcatt
     1441 tcccgggtgt tttatcacct tccaacggga tacaacggat ggtagccttg gtttcgttct
     1501 tgatgagctc ttcggtttcg ggggttccgt cccaatgaca gagtagaaat ccgcctttct
     1561 cgatctcttc tttaaattct tcgtaggagt ttacttcgcg ggtggtggag gcgcggtaat
     1621 cgagcgcttt cttatagatg ttttcctgaa tttcatcgag caggttcttg acgtattcct
     1681 cgatgttctc gcaggaaacg gtctctttgg tcagcgtatc gcggcgggca acctctatgg
     1741 tgttgttctc catatcccgg ccacccatgg ccagacgaac gggaacacct tttaattcgt
     1801 attcggaaaa cttccacccc ggtttcttgt tgtcggagtt atcgtacttt acgcttactc
     1861 ccagcgcttt caggcgtgag acaatacccg ataccttttc gtctatttga gccagttgtt
     1921 cggcattttt gtatataggg acgataacaa cctgataggg agccagtttt ggggggagta
     1981 ccaacccgtt gtcgtcgctg tgagccatga tgagtgcccc gataagccgg gtggaaacac
     2041 cccaggaggt agcccagaca tagtcgcgtc cgccgtcctt atcggcaaac attacatcaa
     2101 acgctttggc gaagttctgt cccagaaaat gcgatgtgcc ggattgcagg gcttttccgt
     2161 cctgcatcaa cgcttcgata gtataggtgt ctatggctcc cgcaaatcgt tcagaagccg
     2221 atttctttcc ttttatcacc ggcagggcca tgtacttttc ggcaaaatcg gcgtaaacgt
     2281 tgatcatctt ctcggtctcc tccacagctt cttgctccgt ggcatgagcc gtatggcctt
     2341 cctgccagag aaattcggcc gtgcggagga acagccgggt acgcatctcc caacgaacca
     2401 cgtttgccca ttggttgatg agcaacggca ggtcgcgata cgattgaatc cagtttttat
     2461 aggtgctcca gataatggtt tcggaggtgg ggcggatgat cagttcttcc tccagcttgg
     2521 cctcgggatc taccactacg ccgcttccat cgggagcgtt cttcagccgg taatgcgtta
     2581 ctacggcaca ctccttggca aaaccttcca cgtggtcggc ttcacggctg agaaacgatt
     2641 tgggtataaa caacggaaaa taggcattca cgtgtccggt ttccttgaac atgtcgtcca
     2701 gctggcgttg catcttctcc cagatagcat acccatacgg ttttatcacc atacaaccgc
     2761 gtacggctga gttttccgcc aattcggctt taatcaccag gtcgaggtac cattgcgaat
     2821 aatcttcgct gcgggaagta agttctttca attcctttgc catatcttat ataaaattct
     2881 gaatatttat ttaagggtgc aaagatacga atattcgtag aaatttgaaa ttagaaacgg
     2941 gtatttcgac ttaagaagat cccggttcta tccaaaatta ctttttatct tatccagcag
     3001 gttcccgcag gcatcttcca gcaaatccat caccagctcg aaaccggacg ctcccccgta
     3061 atagggatcg ggaaccttgt ggtgatggga aaacttactg gagaaatcga tcatccggtg
     3121 gattttggca acatcctctt ccgtttttgc cagttttttt acgttcctgt agttgtcatc
     3181 atccatcaca atgatgtaat cgaaccgctc aaaatcagtg ctgacgaact tccgcgaccg
     3241 ggaacaaaaa tcgtagttgc gccgttgtcc gtgcatacgc atccggtcat cgggcaattc
     3301 gccttcgtgc cagccggatg tgcctgcgga atctacaaag attctctctt ccaggcggtt
     3361 gtcggcaacc attttcttga gaatcccttc agcagccgga gaacggcaaa tgtttccgag
     3421 acaaacaaag agaacgttga cttgttgatt cttggcagga ggaatttgca taacgctaat
     3481 cgattataat ctaaaacaat gcggcaaaga taacattttt atttatattt gcagccttaa
     3541 accatttttg tgtaggaatg aacataccgg aagagagaaa atcgcagccc atttccaaag
     3601 agattaccgt tgttgaagct aaaaaagaat tggagaagaa caggagtaaa agaatcctgt
     3661 tcgccacagg aggttctttt gccctggttt tagccgtgtt gggcgttttc gttcccggca
     3721 taccctgtac ccctttcgca ctattgtctg ccgccctttt tgcaaaaagt tccgaaaagc
     3781 tgtataactg gttgctgagt aacaggatac tggggccccg cattaaaaac taccagcgcc
     3841 ggaaaggtat ttcgaaaggc ggaaaagtga aggtaatcat tttgatggtg acgatggtgt
     3901 tggtctcttc tttcctgata ataaatgtgc cggccataag gatcgtaatt ctcttgtcgg
     3961 gccttgtcgg agcgattgtg gtgtggttct ttgttcccga agggagagac tataccgaag
     4021 aggagggcta gtccctcagg tacgcgaagt aatacttctc cacctttttc gataacaact
     4081 cgatgttgaa catatcggat gcggtggaga tgtcttttat ggagccgtcg cgatagagga
     4141 tcttgatgct ttcgtcgtac ttgttgtaca tgtcggtagc cagcgaatcg gcagaaacaa
     4201 aataggcagc ctcgctttcc gagattccgt atttacgggt aaatttttcg ataaattctt
     4261 gcttcttttc tgtcgggtga ggttcattgg taatctggat cttgaacagc ttcctgttga
     4321 ccattccgtg gctcagcaag gataagacct tgtcggaatg agacttccat acttttaacg
     4381 atgtccagat atcgttgtcg tccaggttcg tgaaatgttc cagcacttcc gggctttccc
     4441 ggaaatcttt caacgtgatg tcgtttttta ggaagaatgc cagcgagggc gatgcaaaga
     4501 ggtcttcgcc ctttcgcgac aggtatttgg cccggttgat ggtgtttgtc aacattttct
     4561 ccgaggccac cgccgttttg tgcaggtaga cctgccaata cataaaccgg cgtgacatca
     4621 ggaagttctc tatcgaataa atccctttcg actcaaccac cagcttgtcg tcaatcacat
     4681 cgagcatctt catgatgcgt gccgcaccga tacccccttc gctgacaccc gtaaaaaaac
     4741 tgtcgcgctg caggtagtcc agccggtcga catccaactg cccacttacc agctcgtgta
     4801 aaaaccgctt ggggtacttg tcccggaaaa tatcgatggc cgtctgaagt gcaccttttt
     4861 tctcgccgtt gatctgctgc atcagcagca acgaaacctc ttcgtgaggc acgttgttcg
     4921 ccagcgtatt ctccagcaca tgcgaaaagg gagtgtgccc gatatcgtgc atcaggatgg
     4981 cagccagcgc tccgcggtat tcgtctttgg tgatctcgtg gcctttgtcg cgtaaattca
     5041 gcaaggccac gtccatcagg tacatggcgc caatggtatg gtgaaaccgg gtatgctgtg
     5101 caccgggata aacataggat gccattccca gttgccggat ccggttcagc cgctgcaggc
     5161 agggatgctg gataagttcg taaataaact catcggggat attgacaaat ccaaaaacgg
     5221 gatcgttgat tatttttcgc ttctgctgca tatagaataa aaaaagactg ctggctttcc
     5281 tccaacagtc tgatttttaa ttagttgtct taccaggact cgaacctgga caaacaggac
     5341 cagaatctgt tgtgctacca ttacaccata agacaatttg tgagtacaaa agtacaaaat
     5401 ttattgtaac aaaaaattat taggctgaaa cttttgtttg attcgtgaat taaaattgct
     5461 atttttaccg agcaacaacg tttttacggg ttaatcccgg tagcttccag aaccaccgtt
     5521 ttctctttca gaaattccat caacgttgcg taaacaggat gttttttaag gatttgccgg
     5581 tttcccacca taaacagttg ttggcgcgcg cgcgtaagag atacgttcag ttttctgtct
     5641 acggtgccct catcattcag gttacaaaga aagtgcagtt gatacggttt attcacacaa
     5701 aacgaaatga tgatcacatc gcgctggctg ccctggaagc gttccaccgt atccaccatg
     5761 atgttctcgt acgccggaat gcctgctttc tccagttggt gcttgatgag tgctatctga
     5821 tttcgatagg gcgcaatgat tccgatattc cgtgggtcga aattccgccg gtcgtgcgca
     5881 tacacggtgg caacggaacg tatgacctca gccacgatct ccgcctcagg ggtgtttgtt
     5941 ttcgatgagg tactttgcgg aggcaactgt tcggtcgaaa aaaatgcgat tcggcttttt
     6001 gacacatgct gtaaaaagac attgccggtt tcggggaagg atgatctcca cggttgagcc
     6061 tgccactcct ttgcggacaa aagggcttgc gggtaaaagt gagtggaggg gaatgcggca
     6121 atctgctcgt gcatcctgcc ctggatggtg agctgggtgt aggcgtgtgt ccagttgtta
     6181 cggacgcagt tgcgcagcaa gcgttcgaaa agggaatggg cacaactggt gatccctgct
     6241 ccggtaagtt ccggctcgtg aatggcggca acctgttttt tctgcaagac aatggcgggc
     6301 aactggtgat ggtcgccaat catgatgaac ttatcgaaac ggggcaacaa ccctataatt
     6361 tgtggctcca ggatctgtga cgcttcatcg ataatggcca catcgaactt cttgaggctg
     6421 aaaagttccg gtttcccgtt tatggatgcc agggtggata caacaatccg ggtcttccgg
     6481 atcgttgtcc gcagcgattc gcggttactg gctttttcgg agatgttttg caacaaccgg
     6541 tcccgatatg cttcggcgca cgagagttcg cttcccacac ggatgtagtt gcttgaattt
     6601 tcatccttgc agccaaatgc ggcatttatg gcttcgcaaa gctcatctac ggcccggttg
     6661 gtataggcca ataccaggat gctgccgttt tctttcgtgt aaaactcttc aaccagccga
     6721 cgggcaaaaa tggatgtctt tccggttccc ggcgggccga taatcaggaa atagtcttcg
     6781 gcagccatcg ctttccggat gatgctttcg ggataaggca acttgttttc agggattgcc
     6841 ggagcttggg gtgctcttaa tcccaacagt aaatcgcgct gctgcttttc ggcattcaga
     6901 aaatcaaaca ggctcttata catactgttg tacgatgtgt cgagcgcgtc atgctcgatt
     6961 gcccaaagcg ggttctcgtt gaaaaacgtg cggtttcgtt gcttgttccg gaaacggacc
     7021 tcgacaaatt cccgcgagat ggctgccagg gcccctttca gtatttggcg gttgaggacg
     7081 gtgtcttgtt catcctgccg gggatagaca atgcagatct ctccctcacg gaagtttacc
     7141 acatcgtttc cggcatgatt tctccggaac acgattttca tgtcgtttcc cgaatcgtca
     7201 atcgactcga tggacaagcc atacaaaaca tccagtgctt ctgcacgttc ggtaaaatcg
     7261 ctgttccaca acgaagccac accggtgggt gactcgtatt ctacgtctcc tgttttttgc
     7321 aggtacagtt cgcgtgaaac aaaacgtatg tagcggtaaa agtagctcaa ttccattggc
     7381 gtgcattgtt gcagtaccga cttgaattgt tcgatccgtt gcgtgtagaa cgtcgcggat
     7441 ttctgtgcgg ttccggtggt gtcgtaaagt gcctgaaaca gttgcgcaac cgtttgattg
     7501 ttaccgttta tgatggcatg ttcgttggca ataatgaggt tcctgacgtt gatgatctct
     7561 ttctccagtt gttggaatcc ggcggcaaaa cggaggtttg tacccggaat gcttccggac
     7621 gagtacaaaa tggctgcttc cacccggcgt gacggtacat caaacacact ttcggtcatt
     7681 agccggtaaa cgccggtctg tacttcgtgg ttaagcgcaa tctttcctgt gtcgtaagca
     7741 gggtagggaa gtttacccga tttgagctct acaatctcat acgctttttt attgatgtgc
     7801 aacaagtcca accgcccctg aaaaccgtat cgttcactga agaacgacgg ttcgagcgta
//
LOCUS       DAOSDR010000104         7852 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrimonas sp. isolate AMR_MDS_1826
            NODE_3411_length_7852_cov_4.842375, whole genome shotgun sequence.
ACCESSION   DAOSDR010000104 DAOSDR010000000
VERSION     DAOSDR010000104.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863355
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrimonas sp. (wastewater metagenome)
  ORGANISM  Petrimonas sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Dysgonomonadaceae; Petrimonas.
REFERENCE   1  (bases 1 to 7852)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7852)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:10:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,720
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 35
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7852
                     /organism="Petrimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3411_length_7852_cov_4.842375"
                     /isolate="AMR_MDS_1826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2023866"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..352)
                     /locus_tag="PLI30_05540"
     CDS             complement(<1..352)
                     /locus_tag="PLI30_05540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019538967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HOI79052.1"
                     /translation="MIENNLPYKKKEYKYILIGFLILLGLILFKELRLYLSGFLGAAT
                     LYVLLKGQMTYLVEKKKIKKGFAATLLTLEALLLFLGPLTGIAFLVIDTVSGISIDPK
                     AIIENFNNFVKMIEE"
     gene            complement(381..614)
                     /locus_tag="PLI30_05545"
     CDS             complement(381..614)
                     /locus_tag="PLI30_05545"
                     /inference="COORDINATES: protein motif:HMM:NF020967.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2007 domain-containing protein"
                     /protein_id="HOI79053.1"
                     /translation="MEEEFITVKTFAFPADVSIVQTFMEMKGIEVFMKNLTASRLAYS
                     IGDIEMQVKASDYDAAKNALIEGGFAEPEDFFL"
     gene            724..1482
                     /locus_tag="PLI30_05550"
     CDS             724..1482
                     /locus_tag="PLI30_05550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018360736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOI79054.1"
                     /translation="MKRKIALITGATSGIGEATTWRLAENGYDVIITGRRNERLKDVK
                     EKLQKAGTRVLALCFDVRVEFEVKSAIENLPDDWKKIDVLVNNAGLAAGLSTLQEGDT
                     GDWNRMIDTNVKGLLYMTRYVSPLMIKQEKGHIINIGSIAGKEVYPNGNVYCATKHAV
                     DALTKGMRIDMLKHNIKVTQVCPGAVETEFSIVRFHGDEERAAKVYDGFDNLLADDIA
                     DCILFAVSRPAHVNINDMIVMPTAQATASLFYKK"
     gene            complement(1488..2003)
                     /locus_tag="PLI30_05555"
     CDS             complement(1488..2003)
                     /locus_tag="PLI30_05555"
                     /inference="COORDINATES: protein motif:HMM:NF014678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF192 domain-containing protein"
                     /protein_id="HOI79055.1"
                     /translation="MKSKKLLIIGFIAVVAVAATLFFLTQKKDTKITDGGAFEISFNK
                     QGTLAFLSAQNNDTLSVIDIEVADNNQRRARGLMYRKSLPADAGMLFVFDEEEIQGFW
                     MKNTYIPLDMLFVNADNEIITIHTNTAPLKEWNYASTRPALYVVEVNAGYCAQKQITE
                     GDKIIFELSSH"
     gene            complement(2063..3058)
                     /gene="cbiB"
                     /locus_tag="PLI30_05560"
     CDS             complement(2063..3058)
                     /gene="cbiB"
                     /locus_tag="PLI30_05560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019538974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016880 - acid-ammonia (or amide) ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-phosphate synthase CbiB"
                     /protein_id="HOI79056.1"
                     /translation="MIHDFITTHSGFLIPLAGGFLLDALLGDPARLPHPIRLFGKMIS
                     FCELKFNKGKRRRTKGIAVASILVLLVFGFLYGIQLALHDFPAVKVVINTVFFFYALG
                     NRSLIEEAWKVERRVQKNDLSAARRQLSRIVGRDTSQLSFQQIRTATLETLAENLSDG
                     VVAPLFFYALGGIPLMMAYKMINTLDSMIGYKNDRYNDFGWFAAHILDDGANYVPARL
                     TAFLMISFPPSSRGFHFVHKYASRHSSPNSGYPESALAGILNCRFGGSNTYDGKRVEK
                     PYIGKNYRELTHDDVVKSCIINIRTSLLMLICTLSMYCIFQELTGFSHNFLYFWG"
     gene            complement(3055..4086)
                     /locus_tag="PLI30_05565"
     CDS             complement(3055..4086)
                     /locus_tag="PLI30_05565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007364633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HOI79057.1"
                     /translation="MLYGHGNDLYDYSEKITADFSSNIPYRNASVQIVAHLSGCLHLI
                     SDYPDPNARALTHEIAEHHNLSPENVLVTNGSAEAFYLLAHLFAGKHSTITYPAFAEY
                     EDACTLYGHTLSFMPVSELCGSINFTSNETVWFAFPNNPDGSIVSSETIENLCLNNPD
                     SYFIIDNAYGELCTQCQPVIPFHANYPNLISVHSLTKTFAIPGLRLGYIVAEKNIINR
                     LQPLRIPWTVNTLAQEAGSFVMENYSRLLPDAGILCRESQQFQQQLATLSHLEVTPSP
                     CHFFLIRLKSGTASQLKSYLLEKHGILIRDASNFRGLSPSHFRLSVQEKTANDRLTDA
                     LRDFFKITA"
     gene            complement(4091..5551)
                     /locus_tag="PLI30_05570"
     CDS             complement(4091..5551)
                     /locus_tag="PLI30_05570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019538976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobyric acid synthase"
                     /protein_id="HOI79058.1"
                     /translation="MQKLRPLMFVGTGSDVGKSVINTGFCRIFLQDGYSPAPFKAQNM
                     SLNSYVTADNLEIGRAQAVQAEACGIGCLVEMNPILLKPNDDLTSQVVLNGKPAGNKT
                     ASEYFNEAGRDTLFNEVMHSFNRLAGQYNPIVIEGAGSISEVNLWDKDIVNMRVALHT
                     QAATFLVADIDRGGVFASVYGTLQLLPQAERDAIKGIVINKFRGDLKLFEEGKKILEK
                     LTAKPVVGIVPYFQGLFIEQEDGVVIEQKKKSHEHGKINIGVVLLKHLSNFTDFNMLE
                     QTPGVNLYYTGCPETLARADIIIIPGSKNTLSDLKALREKGLEEVILAHHNARKPVYG
                     ICGGYQMMGIEVNDPNGVEGNVPSLPGLGILPVTTTLASGKKTRQCTFSFVQGNASGN
                     GYEVHAGHTPSIRPLCRMNNGEYDGYCLNARTWGTYIHGIFDNASVINHVLRQLIPGF
                     TSTIDYKARKEQGYNQLADLIRENVDMEYIYKCLKT"
     gene            complement(5559..6092)
                     /gene="cobC"
                     /locus_tag="PLI30_05575"
     CDS             complement(5559..6092)
                     /gene="cobC"
                     /locus_tag="PLI30_05575"
                     /inference="COORDINATES: protein motif:HMM:NF012521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043755 - alpha-ribazole phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ribazole phosphatase"
                     /protein_id="HOI79059.1"
                     /translation="MKLYLVRHTRVNMPPGICYGNSDVALADTFPEELSAIQSELEGI
                     RLARVYSSPLKRCVTLAREFSGNVIVDGRIREFDFGKWERQPWDDIYAQDKGKEWFDD
                     YVNTSCPHGESFRMMLERVRRFIDSLPKTGGNILIVTHAGIIRAFLILLEGYTVNEAF
                     DRKIAYGEIIILEKQKK"
     gene            complement(6096..6851)
                     /gene="cobS"
                     /locus_tag="PLI30_05580"
     CDS             complement(6096..6851)
                     /gene="cobS"
                     /locus_tag="PLI30_05580"
                     /EC_number="2.7.8.26"
                     /inference="COORDINATES: protein motif:HMM:TIGR00317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008818 - cobalamin 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-GDP ribazoletransferase"
                     /protein_id="HOI79060.1"
                     /translation="MKQQLNLFFHALQFYSRIPAGKIDYSEENLAQSIRYFPLVGSVV
                     GIVGASVFMLFMLVFSQGVSVTAALISMIAVTGALHEDGVSDFFDGFGGAYTRERILE
                     IMKDSHVGAYGVISLVLLLLAKFSLLTSITPEKVPLVLIAAHTSSRFVAVALMKSSSY
                     ARTENSKSAHSRLSLSRTTVVTAFVFGVLPLVFIPPLVSAVAAIVYAIILFALKHYVE
                     KKIGGFTGDVLGTLQQFCEIAFYLVYTALISLI"
     gene            complement(6861..7844)
                     /gene="cobT"
                     /locus_tag="PLI30_05585"
     CDS             complement(6861..7844)
                     /gene="cobT"
                     /locus_tag="PLI30_05585"
                     /EC_number="2.4.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004330493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008939 -
                     nicotinate-nucleotide-dimethylbenzimidazole
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide--dimethylbenzimidazole
                     phosphoribosyltransferase"
                     /protein_id="HOI79061.1"
                     /translation="MNNRTKPLGSLGKLEKLALKIGTVQNTLTPELKNPAILVFAADH
                     GIADEGVSPCPREITWQMVMNFLRGGAGINVFSRQHNIRLRVIDAGVDYDFPAGCNVE
                     NRKLARGTKNMIHEPAMSIELCRQAMDKGAECVRQEFERGCNIIGFGEMGIGNTSPAS
                     LLLHKFAGIPLDECVGRGAGLNEEGVRHKYNVLRQVTAKYNPRTPLETLAVFGGLEIA
                     MICGGVLEAKRLNMLIIADGFIASSGFLTAYEMQPDVLDNVIFSHASNEHGHKAMVEY
                     MKGDPVLHLDLRLGEGTGVALAYPVLQSALLFLNEMASFEDAAVFDVEKNR"
BASE COUNT         2029 a         1829 c         1902 g         2092 t
ORIGIN      
        1 gctcttcaat catcttaacg aagttgttga agttctctat tatggctttg gggtcaatag
       61 aaattcccga tacggtatcg atcaccaaaa aggcgatgcc tgtaagtggc cctaaaaaca
      121 acaatagggc ttccagggtc aggagggtgg ccgcaaaccc tttttttatc ttcttttttt
      181 ctaccaggta ggtcatctgt ccttttaaaa gcacataaag cgtggcagct cctaaaaaac
      241 cgctcaggta aaggcgaagt tctttgaaaa ggatcagtcc gagcaggatc agaaacccaa
      301 tgagtatgta tttgtattct ttctttttat aagggagatt attctcaatc atgtattttt
      361 agtgattaaa aaaatccaac ttaaagaaaa aagtcttccg gttcggcaaa accgccttcg
      421 ataagtgcat ttttagcagc atcgtaatcc gaagccttta cctgcatctc gatatcccca
      481 atgctatagg ccagccggct agctgttaaa tttttcataa atacttcaat acctttcatt
      541 tccatgaatg tttgaacgat ggatacatcg gcagggaaag caaatgtttt gacggtaata
      601 aactcttctt ccataaagct ggtatttgtt tagtagactt caaaaatagt ttattttttg
      661 atgataaggg tcaaaaactc cattttttga tgtaattttg attttattat aattttatac
      721 gttatgaaga gaaagattgc actaataacc ggtgccacat cggggatagg cgaagctacg
      781 acctggagat tagccgaaaa tggctatgac gttatcatca ccggacgcag aaacgagaga
      841 ttaaaagatg taaaagaaaa acttcaaaaa gcggggaccc gtgtgttggc cctttgtttt
      901 gatgttcgtg ttgaatttga agtaaaatcg gcgattgaaa atttgccgga tgactggaaa
      961 aaaatcgatg tattggtcaa caatgcggga ctggcagctg gattaagtac cctgcaggaa
     1021 ggagataccg gtgactggaa tcgaatgatc gacaccaacg ttaagggact gctctacatg
     1081 acccgctacg tttctcccct gatgataaaa caggagaaag gacacatcat caacatcgga
     1141 tcgattgcag gaaaagaagt ctatcctaat ggaaatgtct attgtgctac caagcatgca
     1201 gtggatgcgc tgacaaaagg gatgcggata gatatgctga agcacaacat aaaggttacc
     1261 caggtttgtc ccggtgcggt tgaaacggaa ttttccatcg ttcgttttca cggggatgaa
     1321 gaacgtgccg ccaaagtata tgatgggttt gataaccttt tagccgatga tattgccgat
     1381 tgtattctat ttgccgtttc ccgcccggcg catgtaaaca tcaacgatat gattgtgatg
     1441 ccaaccgcgc aggcaacagc atcgttattt tataaaaaat aggtggttca atggcttgaa
     1501 agctcaaaaa taattttatc cccttcggta atttgttttt gggcacaata tccggcgttt
     1561 acttccacca cgtacaatgc cggccgggtg gatgcatagt tccattcttt taagggagcg
     1621 gtgtttgtat gaatggtaat gatttcatta tcggcgttga cgaaaagcat atccagggga
     1681 atataggtgt ttttcatcca gaaaccctga atttcctcct catcgaaaac gaacaacatc
     1741 ccggcatcgg cgggaaggga cttccggtac atcaatccac gtgcccggcg ctggttgttg
     1801 tccgctactt caatatcgat aaccgagagg gtgtcgttgt tttgcgccga caaaaacgct
     1861 agcgttcctt gtttgttgaa ggaaatttca aaagcgccac cgtccgttat tttcgtgtct
     1921 tttttttgtg tcaaaaaaaa caaggtggct gcaacggcta ccacagctat gaacccaata
     1981 ataagcaatt tttttgattt catgcgatac tattttttta tcagcgtcgt gtggatcaaa
     2041 tttaggacca agaacgcaag tactaccccc aaaagtataa aaaattatgc gaaaaaccgg
     2101 ttaattcttg aaaaatacaa tacatggaca atgtgcagat taacataaga aggcttgtcc
     2161 tgatattaat gatgcacgat ttcaccacat cgtcgtgtgt gagttcccgg tagttttttc
     2221 cgatatacgg cttttccacc cgtttcccgt cgtatgtgtt cgagccaccg aaacggcaat
     2281 tcaggatgcc tgccagcgcc gattcgggat aacccgagtt gggactggaa tgccggctcg
     2341 catatttgtg gacaaagtga aacccgcggg atgacggcgg gaaggaaatc atcaaaaatg
     2401 ccgtaagcct tgcggggaca tagttcgcac cgtcatcaag tatatgggcg gcgaaccatc
     2461 cgaaatcgtt gtaccggtcg ttcttatagc caatcatcga atccagggtg tttatcattt
     2521 tgtatgccat catcagcgga atacctccga gggcatagaa aaacagcggt gcaaccacac
     2581 cgtcgctcag attctcagcc agtgtttcga gtgtggcggt acggatctgt tggaacgaga
     2641 gctgcgatgt gtccctccct acaatccggc tcaattgccg gcgagcggcg gacaaatcgt
     2701 ttttttgaac ccggcgttcc actttccagg cctcctcaat caggctccgg ttaccgaggg
     2761 cgtaaaaaaa gaacacggtg tttatgacga cttttaccgc aggaaaatcg tgcaacgcga
     2821 gctgaatgcc gtataaaaac ccgaaaacga gtaaaacaag tatcgatgca accgcaatgc
     2881 cttttgtcct tctccgcttg cctttgttga attttagctc gcagaaagaa atcattttcc
     2941 cgaacaaccg gatgggatgg ggaagccggg cagggtcgcc caacagggcg tcgagcagaa
     3001 aaccacccgc gagcgggatc aggaaaccgg aatgtgttgt gataaaatcg tgaatcatgc
     3061 tgttattttg aagaaatcgc ggagggcatc cgtcaggcgg tcatttgccg ttttttcctg
     3121 tacggacaac cggaaatgag agggtgacag cccgcgaaaa ttactggcgt cgcggatcag
     3181 gataccatgc ttctcgagaa gataggattt taattgcgaa gccgtaccgc ttttcagccg
     3241 aatcaaaaaa aaatgacacg gcgacggcgt tacttcaaga tgtgaaagtg tggcgagttg
     3301 ctgttggaac tgttgcgatt cacggcataa tatgccggca tcgggtagta aacggctgta
     3361 attttccatt acaaaacttc ctgcttcctg tgccagcgta ttaacagtcc aggggatacg
     3421 taggggttgg agccgattta tgatattttt ttcggcgaca atataaccca agcgtaaacc
     3481 gggaatagcg aaagtctttg tgagcgaatg caccgaaatc aggttcggat aatttgcgtg
     3541 aaacggtata accggctggc attgcgtgca caattcaccg taagcgttat cgatgatgaa
     3601 gtaagagtcc ggattattga gacagaggtt ttctatggtt tcagatgaaa cgattgagcc
     3661 gtcggggttg ttggggaagg cgaaccaaac ggtctcgtta ctggtgaaat tgatgcttcc
     3721 acacaattcg ctcaccggca taaacgacag ggtatgcccg tacagggtac aggcatcctc
     3781 gtactccgca aaagcgggat aggttatcgt gctgtgcttc ccggcaaaca ggtgtgccag
     3841 aagataaaac gcttctgccg atccgttggt aaccagcacg ttttccggag acaggttgtg
     3901 gtgctcagca atctcgtgtg tgagcgcgcg cgcatttgga tcgggataat cactgatcag
     3961 gtgcaggcaa cccgacaaat gcgccacaat ttgaaccgaa gcattccggt aagggatgtt
     4021 tgaactaaaa tcggcggtta tcttttcgct gtagtcgtac aggtcgtttc cgtggccgta
     4081 gagcataatc ttatgttttc aagcatttat agatgtattc catatccacg ttttcccgga
     4141 tcagatccgc tagctggttg tacccctgtt cttttcgggc cttgtagtcg atggtggatg
     4201 tgaacccggg aattagttgc cgcaatacat gattgataac cgaagcattg tcgaaaatgc
     4261 cgtggatgta tgttccccac gtacgggcat tcaggcaata gccgtcatat tcaccgttgt
     4321 tcatgcgaca aagcggacgg atggaaggag tatgtccggc atgaacttca tatccattgc
     4381 ccgacgcatt gccttgaaca aaagaaaacg tgcattgccg cgttttcttt ccgcttgcca
     4441 gcgtggtggt tacgggcaga attccgagcc cgggcagaga gggtacattc ccttccacac
     4501 cgttagggtc gttcacctct atccccatca tctgatatcc tccacaaata ccgtaaacag
     4561 gtttccgggc gttgtggtgt gcgagaataa cttcttccag gcctttttcg cgtaatgctt
     4621 tcagatcgga aagtgtgttt ttcgaaccgg gaataataat gatatctgct ctagccagcg
     4681 tttcgggaca gccagtataa taaaggttca cccccggtgt ctgttccagc atgttgaaat
     4741 cggtaaaatt cgataaatgt ttgagcaaca ctaccccgat attgattttc ccgtgttcgt
     4801 gactcttctt cttctgttca ataaccacac cgtcttcctg ttcaatgaac aatccctgaa
     4861 aatacggcac aattcctact acgggttttg ccgtaagttt ctccaatatc ttcttaccct
     4921 cttcaaataa ctttaaatcg ccccggaact tattgataac aatgcctttt atcgcatccc
     4981 gttcggcttg cggcaagagc tgcaaggtgc cgtaaacact cgcaaaaacg ccgccgcggt
     5041 caatgtctgc gaccagaaac gttgcggcct gggtatgcaa cgctacacgc atgttgacga
     5101 tgtccttgtc ccacaggttg acttccgaaa tgctgcccgc accttcaatc acaatgggat
     5161 tgtattgtcc ggcaaggcgg ttgaaagagt gcattacttc gttaaaaaga gtgtcgcgcc
     5221 cggcctcgtt gaaatattcc gatgctgtct tgttgccggc gggtttaccg tttaatacca
     5281 cctgcgatgt gaggtcgtcg ttgggtttaa gcagtatagg attcatttcc acgaggcagc
     5341 cgattccgca ggcctcggct tgtaccgctt gtgcacggcc aatttctagg ttatctgcgg
     5401 tgacataact gttgagtgac atgttttgcg ctttgaacgg cgcgggacta taaccgtctt
     5461 gcaggaagat acggcagaaa ccggtgttga tgaccgactt tccgacatcg gatccggtgc
     5521 cgacaaacat taagggacgg agtttttgca tctgttttct acttcttttg tttttccagg
     5581 atgattatct caccgtatgc gattttccgg tcaaatgctt cgtttaccgt ataaccctcc
     5641 agcaggataa ggaatgcccg gatgattccg gcgtgggtga caatcaggat atttccaccg
     5701 gttttcggaa gcgaatcgat aaaccggcgt acacgttcga gcatcatgcg gaacgattca
     5761 ccgtggggac aggatgtgtt cacgtaatcg tcaaaccatt cctttccttt atcctgcgca
     5821 taaatatcgt cccacggctg gcgttcccat tttccgaaat caaattccct gatccttccg
     5881 tcgacaatca cgttgccgga aaactccctg gccagcgtta cacatctttt caacgggctg
     5941 ctgtagactc ttgcaagccg gataccttcc agttccgatt gtatggcgga caactcttcg
     6001 gggaaagtat cggccaaggc tacatcggaa tttccgtagc aaattcctgg gggcatattt
     6061 acgcgggtat gtcttaccaa ataaagtttc atcggttaaa ttaaggaaat cagtgctgtg
     6121 taaacaaggt agaatgcaat ttcacaaaat tgttgcagtg tccccaggac atctcccgtg
     6181 aatccgccga ttttcttctc cacgtagtgt ttcagcgcga agaggataat tgcgtagaca
     6241 atggctgcaa cggcacttac aagcggagga ataaagacca gcggcagcac cccgaaaacg
     6301 aacgccgtaa ccactgtcgt tcgcgataaa gacaggcggg aatgcgctga tttgctgttt
     6361 tctgtgcggg catagctaga cgatttcatc agggccaccg ccacaaaacg gctggaagtg
     6421 tgcgcggcga tcagcaccag cggaactttt tcaggggtga tggatgtcag caacgaaaac
     6481 ttagccagga gtagcagcac gagcgaaata acgccgtagg cgcccacgtg gctatccttc
     6541 ataatttcca ggatacgttc ccgggtgtag gctccgccaa atccgtcaaa aaagtcggaa
     6601 acaccatctt catgcagggc gccggttacg gctatcatag aaataagggc tgccgtgacc
     6661 gagactccct gtgaaaaaac gagcatgaac agcataaata cagatgcgcc gacaatccca
     6721 accacactgc ctaccagcgg aaaataacgg atcgattgcg ccaggttttc ttcactgtag
     6781 tcgatttttc ctgccggaat gcggctgtag aactgcagcg cgtgaaagaa gaggttgagt
     6841 tgctgtttca ttgttctggc ttacctgttt ttctctacgt caaacacagc ggcatcttca
     6901 aagcttgcca tttcgttcaa aaaaagtaat gccgactgca gaacaggata agccagcgct
     6961 actcccgtgc cttctcccag gcgaaggtcg agatgcaata cagggtctcc cttcatgtat
     7021 tctaccatgg ccttgtgccc gtgttcgttg gatgcatggc tgaatatcac gttgtcgagt
     7081 acatcgggct gcatctcgta cgcggttaaa aatccagagg atgcgataaa tccatcggca
     7141 ataataagca tgttcaaccg tttggcctcc aatacaccgc cgcagatcat ggctatttcc
     7201 aatcctccga aaacagcgag ggtttcaagc ggcgttcttg gattatattt tgcggttact
     7261 tgtctcagca cattgtattt gtgccgtacc ccttcttcgt ttaatcccgc acctcgtccc
     7321 acacattcgt cgagagggat acctgcaaat ttgtgtaaca acagtgatgc cggtgacgta
     7381 tttccgatac ccatttcccc aaaaccgatg atgttgcagc cgcgttcaaa ctcctggcgt
     7441 acacattcgg cgcctttatc cattgcctgc cggcagagtt cgatgctcat ggccggctcg
     7501 tgtatcatgt tttttgtgcc gcgggccagc ttgcggtttt ccacgttaca ccccgcggga
     7561 aaatcgtaat ctacgccggc atctattaca cgcaggcgga tgttatgttg ccgggaaaat
     7621 acgttgatgc ccgcaccgcc gcgtaggaaa ttcatgacca tttgccaggt aatttctctg
     7681 ggacaagggc ttactccttc gtcggcaatg ccgtggtcgg ctgcgaaaac aagaatagca
     7741 gggttcttca actccggagt gagggtgttc tggacggtgc caattttcag ggcgagtttt
     7801 tcgagttttc ccagggaacc cagtggtttg gtacggttgt ttatttttct tt
//
LOCUS       DAOSDR010000105         7840 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrimonas sp. isolate AMR_MDS_1826
            NODE_3417_length_7840_cov_3.886191, whole genome shotgun sequence.
ACCESSION   DAOSDR010000105 DAOSDR010000000
VERSION     DAOSDR010000105.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863355
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrimonas sp. (wastewater metagenome)
  ORGANISM  Petrimonas sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Dysgonomonadaceae; Petrimonas.
REFERENCE   1  (bases 1 to 7840)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7840)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:10:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,720
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 35
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7840
                     /organism="Petrimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3417_length_7840_cov_3.886191"
                     /isolate="AMR_MDS_1826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2023866"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1114
                     /gene="hemN"
                     /locus_tag="PLI30_05590"
     CDS             <1..1114
                     /gene="hemN"
                     /locus_tag="PLI30_05590"
                     /EC_number="1.3.98.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006798207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HOI79062.1"
                     /translation="KREINLVCEKISPGRKISQIHFGGGSPTAIPLSYIKEINSLLLK
                     KFECIDNPEIAIECHPGYMDEHDFELLADAGFNRISIGIQDFNNAVLKACNRKAPQVP
                     IDRIVTALRNRNIHINFDFIYGLPLQTTESFEETIQQAIALSPDRLVTFSYAHVPHIF
                     PRQQLLERNPLPQDELKSNIYENAQKLLLHAGYIQVGIDHFVKQNDELSIARQNHTLH
                     RNFQGYCTRRTTGQVYAFGATAISQLTSAYTQNTKSINEYIETINKGHIPVKKGYALS
                     QEEQILREVITDLMCNNRINWQEIADRVKLSVAEVKSATTYDEKKLQEFADDGITEWS
                     EQEIRMKQKGSPFVRNVAASLDKLLLQSDKTFSKPI"
     gene            1114..2511
                     /gene="hemG"
                     /locus_tag="PLI30_05595"
     CDS             1114..2511
                     /gene="hemG"
                     /locus_tag="PLI30_05595"
                     /EC_number="1.3.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005646113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004729 - oxygen-dependent
                     protoporphyrinogen oxidase activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protoporphyrinogen oxidase"
                     /protein_id="HOI79063.1"
                     /translation="MISLMEKDVVIIGAGLTGLTVALLLKNRGLSVAILEKSNRVGGQ
                     IQTFHENGFVFESGPNTGASASEEVINLFNVLQPDCEIEFARKESESRWIWKNGQFHP
                     LPNGLWSGIITPLFTFSDKLRILGEPFRKKGKNPDESIAALGARRLGKSFVNYAIDPF
                     LSGVYAGDAETLITRHALPKLYSLEQTYGSFIGGSIKKSCENKRDSRSKTPKGIFSVK
                     GGMENLPKAMQKHIGTENLFLSATGTIVSQNSKNRAWQIECTQNGKGLNFTCRHVITT
                     VGAYALPPLLPFIPYNEIEKISNLRYAPIVQVAVGVKEREELTFSAFGGLVSSKDKED
                     FLGILFPFSCFDKRCPENGALFSFFMGGTKRVDLTQLSDTEIEEKTVKAFHRMLKFPE
                     NKMPDLIKIFRHPNAIPQYEISSEERLKTISELEKKFPGLHIAGNLRDGVGMAHRIIQ
                     GTKLANEINQKSHIL"
     gene            complement(2499..3272)
                     /locus_tag="PLI30_05600"
     CDS             complement(2499..3272)
                     /locus_tag="PLI30_05600"
                     /inference="COORDINATES: protein motif:HMM:NF015084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoate A/B protein ligase family
                     protein"
                     /protein_id="HOI79064.1"
                     /translation="MTFVSPLMLDKFNLNTKLIFSPSTVPAFNLAAEEYLFSVREDYL
                     FLYVNNASVIVGYNQAVVNEVDMDFCIEKDIRIIRRLSGGGAVYHDSGNLNYAFISDK
                     KEMPLSADFLLPVIHVLQQLNIPVETGKRKDLWLPGGYKVSGTASHVSKGRELHHGTL
                     LYESNLEHLKRSLSPEKRNLITKATASVPSPVKNISSFLKEEKRNPFSFREFVKRFVD
                     ESMKYFNIAGLTSFSPAEVEAIETLQREKYSQRTWNYKM"
     gene            complement(3241..4539)
                     /locus_tag="PLI30_05605"
     CDS             complement(3241..4539)
                     /locus_tag="PLI30_05605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009134056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HOI79065.1"
                     /translation="MKQLDIHLMHPVEQINIIIGRIYRSGMTTTSGGNISIRDDNGDI
                     WITPSGVDKGNLTTKDIMCVKKDGSVVGLHKPSSEYPFHRAIYESRPDITAIIHAHPP
                     ALVAFSIAGTVPDTKIVPQAHNVCGDIGFAPYGTPGSEDLGKKIAGVFQDKRFRAVIM
                     ENHGVVLGGTDMMDAYQRFETLEFCCRTIVNAGKLGKVKYLSDEQVASYVNHIPRNIS
                     HFMDVEYPSDERALRTEMVNIIRRSCDQGLMISTYGTVSVRWRNDDFLITPRDVARWD
                     ILPSDIVQIKNGMAEAGKIPSRSVALHQRIYQLNPHINSIICTQPINLMAHAVSGSKF
                     DVRTIPESWIFLQDVPSIPFGLIYNEIDGVAKMFRENRVILVENDSVFVTGDKLLNTF
                     DYLEVAEFSANSLVMASAIGPLKPIGDKEIDDLRIAFNVG"
     gene            complement(4557..5840)
                     /locus_tag="PLI30_05610"
     CDS             complement(4557..5840)
                     /locus_tag="PLI30_05610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019244839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HOI79066.1"
                     /translation="MKTRAVRLYGKKDLRLEEFELPAIKDDEILAKVVSDSLCMSSYK
                     ASSQGAEHKRIPDNVAENPIIIGHEFAGELLEVGSKWAHKFKAGDKFSIQPALYYENG
                     PVGILSAPGYSYSYIGGDATYVVIPNEVMERDCLLAYHGEGYYPASLAEPLSCVIGAM
                     HANYHTTPGSYRHNMEIIDGGKMAILAGVGPMGLAAINFVLRREEVKPSLLVVTDIDQ
                     TRLDRAASIYTVEFAASRGIDLKYINTGKMENPVEELKAISGGAGFDDVFVFAPVKPV
                     VEQGDAILGFDGCLNFFAGPDNPNFSAMLNFYNVHYAYTHIVGTSGGNNDDMVEALDI
                     MSKGLDPAGLVTHIGGLNAVAEATNNLPHIPGGKKLIYTHIEMPLTPISDFASLGETD
                     GLFKKLAEICDRHNGLWSVEAEAYLLSNHPSIRTP"
     gene            complement(5858..7819)
                     /locus_tag="PLI30_05615"
     CDS             complement(5858..7819)
                     /locus_tag="PLI30_05615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019540927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HOI79067.1"
                     /translation="MKEIQDLISISRFYGKDSRFVIAGGGNTSYKNKEKIWVKASGSS
                     LETITEDGFAILDRSLLNPMSGKQYSENAAEREEQVKNDLAAATVTKGKRPSVETSMH
                     NVINYAFVVHLHPTIVNGLMCAVNVTTVLENLFGEKVLYVEYTDPGYVLFKKVDEKIK
                     EYRQKFSEEPQVIWLQNHGVFVAADSIEEIKSIYGSIFATLEKAVSIPLPEDERKVCR
                     CVSEVLPAIRMLVSKDNLKTLKVRRNGLIEYFCESPKNQVAIAKPFTPDAIVYCKSNY
                     LFLNDETPGQVLENAEELIPGFVNKFGYLPKVLLIKGIGLVSVGDNARQCDTILDVFE
                     DAMKVAFLSQSFGGAHPMTQAQIEFIDNWEVENYRRSVAAGNASGRVENKTIVVTGAA
                     QGFGEGIAECLLREGANIVVADLNEEAGRNTVGKFNGLAKSNRAIFVKTNVSEISSLD
                     NLVQETVCHFGGIDCFISNAGVLRAGGLEDMTPENFDFVTKINYNAYFYCTKVVSQVM
                     KLQTKYASSDYFADIIQINSKSGLRGSKANFAYAGGKFGGVGLTQSFALELAPFRIKV
                     NSVCPGNYYEGPLWSDPENGLFVQYLNAGKVPGAKTIDDVKAFYLAQVPMRKGCTPGD
                     VTKGVLYLMEQCGETGQALPVTGGQVMLN"
BASE COUNT         2250 a         1837 c         1625 g         2128 t
ORIGIN      
        1 aaaaagagaa ataaatttgg tctgtgaaaa gataagcccc gggagaaaaa tatcgcaaat
       61 tcattttggt ggagggtcgc ccacggcaat accattatct tacattaagg aaatcaactc
      121 gttgttactg aaaaaattcg aatgcattga caaccccgag atagccatcg aatgtcatcc
      181 gggatacatg gacgagcacg attttgaatt gctggcagat gcaggattca acagaataag
      241 cattggcatt caggacttca acaatgcagt attaaaagcc tgtaaccgta aagcacccca
      301 agtccctatc gatagaatag ttaccgcgct gcgaaacaga aatattcaca tcaatttcga
      361 ttttatttac ggattgcccc tgcaaacaac ggaaagcttc gaagagacaa ttcaacaagc
      421 catcgcactt tctcccgacc gccttgtaac tttttcttat gctcacgttc cgcatatatt
      481 tccacggcag caacttctcg aaagaaatcc attaccacag gacgaactga aaagtaacat
      541 ctacgaaaat gcacaaaaac tattgcttca cgccggctat attcaagtag gaatagacca
      601 ttttgtcaaa caaaacgacg aattatccat tgcccggcaa aaccatacac tgcatcgaaa
      661 ttttcaggga tattgtaccc gacgtacaac cggacaggtt tatgcttttg gcgccacagc
      721 catttcgcaa cttacctctg cttatacaca aaataccaaa agcataaacg aatacataga
      781 aaccataaac aaaggacata ttccggtaaa aaaaggatat gcgcttagcc aagaagaaca
      841 aatcttacgc gaagtcatca cggatcttat gtgtaataac aggataaact ggcaagaaat
      901 agccgacaga gttaagctat ctgttgcaga agtaaaatct gcaacgacgt acgacgaaaa
      961 aaaattacag gaatttgctg atgacggcat cactgagtgg tctgaacagg aaataagaat
     1021 gaaacaaaaa ggatctccgt ttgtaagaaa tgtagcggca tcgctcgaca agcttttgct
     1081 gcaatcggat aaaacatttt caaaaccgat ataatgatta gcctaatgga aaaagacgta
     1141 gtaataatag gggccggact taccggatta actgttgctt tacttctgaa aaacagagga
     1201 ttgtcggtag caattcttga aaagagtaat cgcgtaggcg gacaaataca aacatttcat
     1261 gaaaatggct ttgtgtttga gtcggggcca aataccggag caagcgcatc agaagaagtg
     1321 attaatttat ttaatgtact gcagcccgat tgcgaaatag aatttgcccg taaagaatcg
     1381 gaaagcaggt ggatatggaa gaacggacag ttccatccat tgcccaacgg tctgtggagc
     1441 ggcattatta caccgttgtt tacatttagt gataagctgc ggatattggg tgaacctttt
     1501 cggaagaaag gaaaaaatcc agacgagtcc atagcagcgt tgggtgcgcg gcgtttagga
     1561 aaatcgtttg tcaattacgc tatcgacccg ttcttatcgg gagtttacgc cggcgatgcc
     1621 gaaacactca ttacccgtca cgcattgcca aagctctata gtctggaaca aacctacggt
     1681 agttttatcg gaggaagcat aaaaaaatcc tgcgaaaata aaagagactc ccggtctaaa
     1741 actccaaaag gtattttttc ggtgaaaggc ggcatggaga atttacccaa agccatgcaa
     1801 aaacatatcg gaacagaaaa cctgtttctt tctgcaaccg gcaccatcgt ttcacaaaat
     1861 tctaaaaaca gagcatggca gattgaatgc acacaaaacg gaaaaggact taattttacc
     1921 tgccgccacg taataaccac cgtgggagct tatgctttgc cgccactcct accgtttatt
     1981 ccttataacg aaattgaaaa gataagcaat ctgcgctacg ccccaatcgt tcaagtggca
     2041 gtaggagtaa aagaacgtga ggaattaaca ttcagcgctt ttggcggatt agtatcatct
     2101 aaagacaaag aggatttttt gggaattctt tttccgttct cttgttttga caaaagatgt
     2161 ccggaaaacg gcgctctttt ttctttcttt atgggtggaa caaagcgagt agatttaacg
     2221 cagttatctg acacggagat tgaagaaaaa acggtaaaag cttttcatcg catgctgaaa
     2281 ttcccggaaa acaaaatgcc cgatttgatt aaaatatttc gtcatccgaa tgctattccc
     2341 caatatgaaa tctcttcgga agaacgattg aaaacgatat cggaattgga aaaaaaattt
     2401 cccggcttgc atatagccgg taacctgagg gacggagtag gaatggcgca tcgcattatt
     2461 caaggaacaa aattggccaa tgagataaat cagaaaagtc acatcttata attccacgta
     2521 cgttgcgaat atttctcacg ttgcaatgtt tctatagctt ccacctcagc cggagagaat
     2581 gaggttagcc cggcaatgtt aaaatatttc atcgattcat ctacaaaccg ctttacaaat
     2641 tcccggaaag aaaaggggtt ccttttttct tctttcaaaa aagaacttat gtttttcacc
     2701 ggactgggta cggaagccgt tgccttggtt ataaggtttc ttttttccgg acttaacgaa
     2761 cgtttcaaat gttctagatt gctttcgtaa agcaatgtcc cgtgatgcaa ttcacggcct
     2821 tttgacacat gcgacgcggt acccgaaacc ttgtacccac cgggtaacca taagtctttt
     2881 ctctttccgg tttcaacggg aatattcagc tgctgaagca catggatcac gggcaaaaga
     2941 aaatcggcgc tgagcggcat ttctttctta tcggatataa aagcgtagtt caggtttcct
     3001 gaatcgtgat aaacagctcc tccacccgat aaacgccgga taatgcgaat atctttctca
     3061 atacaaaagt ccatgtccac ttcgttcacc accgcctgat tatagccaac aataacactt
     3121 gcattattta catacaggaa gagataatct tcccggacgg aaaaaaggta ttcctccgca
     3181 gccaggttaa aagccggtac ggttgaggga gaaaaaataa gtttcgtatt caaattgaac
     3241 ttatccaaca ttaaaggcga tacgaaggtc atctatctcc ttgtccccga taggtttcaa
     3301 cggtccgata gcagaagcca tcacgagaga attggccgaa aattcggcaa cttccaggta
     3361 atcgaaagtg ttgagcaatt tatctcctgt aacaaagaca ctgtcgttct ccaccaaaat
     3421 cacccggttt tcacgaaaca tttttgctac accgtcaatc tcgttataaa tcagtccgaa
     3481 cggaatcgaa ggcacgtcct gcaggaagat ccaactttcg ggaatcgtac gtacatcaaa
     3541 cttgcttccg ctcacagcgt gtgccatcag gttgatgggt tgagtgcaga taatggaatt
     3601 gatgtgcgga ttaagctgat aaatccgttg gtgaagcgct accgaacggc tgggtatttt
     3661 tcctgcttct gccattccgt ttttgatttg tacaatatcg ctcggcaaaa tatcccaacg
     3721 ggcaacatca cggggcgtta ttaaaaagtc atcgttacgc caccgtacac tcaccgttcc
     3781 atacgtagaa atcatcaagc cctgatcgca tgaccggcga atgatattaa ccatttcggt
     3841 acgtaaagca cgttcatcag acggatattc tacatccata aaatgtgaaa tattcctcgg
     3901 gatatggttc acatagcttg ccacctgttc gtcagaaaga tattttactt ttccgagttt
     3961 acctgcattc acgatggtac ggcagcaaaa ctccaaggtt tcaaaccgct gataagcgtc
     4021 catcatatcg gtcccgccca gcacaacacc gtgattttcc ataattacgg cccggaaacg
     4081 tttgtcttga aacacaccgg caattttttt tccaaggtct tcactcccag gtgtgccata
     4141 aggcgcaaaa ccgatatcgc cacagacatt atgcgcctgc ggaacaatct tggtatcggg
     4201 gacagtgcca gcgatgctga aagcgaccag cgccggtgga tgagcatgaa tgattgccgt
     4261 tatatccgga cgagattcgt aaatcgccct gtggaaagga tattctgaag atggtttgtg
     4321 aaggcctacg acggatccat ctttttttac acacataatg tccttggtgg tcagatttcc
     4381 tttatccacg cccgacggtg tgatccagat atctccgtta tcatcccgga tggaaatatt
     4441 tccaccggaa gtggtggtca ttccgcttcg gtaaattcgt ccgataatta tgttgatctg
     4501 ctctaccgga tgcattaaat gaatgtctag ttgtttcata tgatgccagt ttcgggttat
     4561 ggggtacgga ttgagggatg gttagacaag aggtatgctt ctgcttctac gctccacaaa
     4621 ccgttatgcc ggtcacagat ttcagctaat ttcttaaaga gaccgtcggt ttctcccaaa
     4681 cttgcaaaat cggaaatggg ggtaagcggc atttctatat gtgtataaat caatttttta
     4741 cctccaggaa tatggggcag gttgttagtc gcttcagcaa cagcattcaa gcctccgata
     4801 tgagtaacca atccggcagg atccaaccct ttgctcatga tgtccaatgc ttctaccata
     4861 tcgtcgttat ttccaccgct ggtgccgaca atatgcgtat aggcgtaatg aacattatag
     4921 aaattcaaca tggcactgaa gttcgggttg tcagggccgg caaaaaagtt caggcatccg
     4981 tcaaatccaa gaatagcatc cccttgttct acaacaggct ttacgggagc aaacacaaag
     5041 acatcatcga aacctgctcc gccactgatt gctttcagct cttccacggg attctccatc
     5101 tttcctgtat tgatgtactt cagatcgatt ccgcgtgaag cagcgaactc aacggtatag
     5161 atggatgctg cacggtcgag ccgtgtctgg tcgatatcgg taaccaccag taaggatggc
     5221 tttacttctt cgcgacggag cacgaaattg atcgcggcca gccccattgg acctactccg
     5281 gcaagaatag ccatctttcc gccgtcaatt atttccatat tatgccggta actgcccgga
     5341 gtggtatgat aattggcgtg catggctcca ataacacacg aaagcggttc ggccaatgaa
     5401 gcaggataat acccttcacc gtgataagcc aggaggcaat ctcgctccat cacttcattc
     5461 ggaatcacca cataggttgc gtcacctcca atatacgagt aactataacc aggagcgctc
     5521 agaataccaa caggcccgtt ttcgtaatac agggccggct gaatggagaa tttatcacct
     5581 gctttgaact tatgcgccca cttgctgcca acttccagta attctccggc aaactcatga
     5641 ccgatgatga tggggttttc ggctacatta tcggggatcc gtttgtgttc cgcgccttgc
     5701 gaggatgcct tgtaagacga catacacaat gaatcagaaa ctacttttgc aagtatctcg
     5761 tcgtccttaa tggccggcaa ttcaaactct tccagcctga gatctttttt gccgtacaaa
     5821 cggactgctc ttgttttcat tttttgctat gattttatta attcaacatt acttgtccac
     5881 cggtaacagg caatgcctgt ccagtttcgc cgcattgctc catcagatac agtaccccct
     5941 tggtaacatc cccgggtgtg caaccttttc gcatcggcac ctgtgccaga tagaacgctt
     6001 tcacatcgtc aatagttttg gcaccgggta cttttcctgc attcaggtat tgtacaaaca
     6061 acccattttc gggatcactc cagagcgggc cttcgtagta attgccaggg cagaccgaat
     6121 tgacttttat cctgaaggga gccagttcca gtgcaaagga ctgcgtaagt ccaactcctc
     6181 cgaacttccc accagcatat gcaaagttag ccttgcttcc gcgaagtccc gatttcgaat
     6241 ttatttgaat gatatcggca aaataatcgc ttgatgcata tttcgtttgc agcttcatta
     6301 cttggctcac tacttttgta caatagaaat aggcattgta gttgatcttt gtgacaaagt
     6361 cgaagttttc gggtgtcata tcttctagcc caccggctct caacacaccg gcgttgctaa
     6421 taaagcagtc aattccacca aaatgacaga cagtctcctg caccaggttg tcgaggcttg
     6481 aaatttcgga aacatttgtt ttcacaaaaa ttgcccggtt acttttcgcc aggccattaa
     6541 acttcccaac cgtattccgt cctgcctctt cgttcaaatc tgccactaca atattggctc
     6601 cttcccgcag caaacattcg gcaattccct cgccaaaacc ttgtgctgca ccggttacaa
     6661 caatggtttt attttctacc cgccccgaag catttccggc agcaacactg cggcggtaat
     6721 tttccacttc ccagttatca ataaattcaa tttgagcctg tgtcattggg tgtgcaccgc
     6781 caaacgattg agagagaaag gcaactttca tagcatcctc aaatacgtcg agaatggtat
     6841 cgcattgtct ggcattatct cccacggata caagcccgat gccttttatc aacaaaactt
     6901 ttggcagata accaaactta tttacaaagc cgggaatcaa ttcttcagca ttttcgagaa
     6961 cttgtccagg tgtttcatcg ttcaaaaaca aatagttaga cttgcagtaa acgatcgcat
     7021 cgggagtaaa aggttttgca atggctacct ggtttttcgg actttcgcaa aagtattcta
     7081 tcaatccgtt cctgcgaact ttcagggttt taaggttatc cttagaaacc aacatccgga
     7141 ttgcaggcaa cacttcactc acgcagcggc acaccttcct ctcgtcttcc ggcaatggaa
     7201 tgctcacggc cttttcaagt gttgcaaaaa ttgaaccata tatagatttt atctcttcaa
     7261 tactatccgc agccacaaaa accccgtggt tctgcaacca gattacctgc ggttcttcgc
     7321 tgaatttctg gcgatattcc tttattttct catctacttt tttaaacaga acatagcccg
     7381 gatcggtata ttccacgtac aacacttttt caccgaaaag attttccaac actgtggtga
     7441 catttactgc gcacattaat ccatttacga tcgttggatg taagtgaacc acaaatgcat
     7501 agttgatcac gttgtgcatg gaggtttcta ccgacgggcg tttacctttt gtgacagtag
     7561 ccgcagcaag atcatttttg acttgctctt cgcgttcggc agcattttcg ctgtattgct
     7621 ttccagacat gggattaagt agcgagcgat ccagtatggc aaagccatct tcagtaatgg
     7681 tttcgagtga tgaaccactg gctttcaccc agatcttctc tttgttcttg tacgatgtat
     7741 ttccccctcc ggcgatcaca aaacggctgt ctttgccgta gaatcgcgaa atggatatta
     7801 aatcctgaat ttctttcatc tttattgttt tccgactgtg
//
LOCUS       DAOSDR010000106         7808 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrimonas sp. isolate AMR_MDS_1826
            NODE_3440_length_7808_cov_4.825616, whole genome shotgun sequence.
ACCESSION   DAOSDR010000106 DAOSDR010000000
VERSION     DAOSDR010000106.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863355
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrimonas sp. (wastewater metagenome)
  ORGANISM  Petrimonas sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Dysgonomonadaceae; Petrimonas.
REFERENCE   1  (bases 1 to 7808)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7808)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:10:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,720
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 35
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7808
                     /organism="Petrimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3440_length_7808_cov_4.825616"
                     /isolate="AMR_MDS_1826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2023866"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            79..1194
                     /locus_tag="PLI30_05620"
     CDS             79..1194
                     /locus_tag="PLI30_05620"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HOI79068.1"
                     /translation="MKKFSLLLITAFVLISCQNNKTYKIDGTVTDPAYEGKNVYIQEM
                     TTNEMVSVDTAVVTNGSFKFEGSADSTVLRFIALDETVNPQKPSRVLAILEPGTIRVQ
                     FDSTVTVSGSQLNDAYNTFRTEQENITKEIRTLSQQYQEAASTGAMTDSLDAALTAQY
                     EKLSGDISSKTADFIKANIANPLGKFLFMTSAEMFEPETQREILALTDEAYKSQESIQ
                     RIVKRLENAERVAIGQKFLDFTMKDPEGNDISLSDYAGKGKVVLIDFWAAWCGPCRDE
                     MPNVVAAYNNYKNKGFEVVGVSLDRERERWLSGIKELNMTWPQMSELKFWDTPVVELY
                     AFRGIPHTVLLDKEGIIIAKDLRGAELHNKLNELLGE"
     gene            1333..2427
                     /locus_tag="PLI30_05625"
     CDS             1333..2427
                     /locus_tag="PLI30_05625"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009037934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HOI79069.1"
                     /translation="MLLRLKKLIIYGLGVLFLIPAESAFAQKNGFTVVIDPGHGGRDP
                     GAIGSSSKEKDIVLVVGKKLGELIKNNHPDVKVLYTRDTDRFVELNKRAEIANKAKAD
                     LYISLHCNALDRRKRSPQGVETFVLGLHRSKDNLDVAKAENSVILYEDDYSTKYQGFN
                     PNEPESYIMFEFMTDQYLQQSVYFASLVQNRLVNNSKRTNRDVRQAGFLVLREVAMPS
                     ILVELGYISNPSDEKYMKTESGQRSLANSVYLAFKEYKHEYDKKSHVFTNPEQRKTIA
                     ANPPSTGMDVTGTSEYRIQFLTAPRKLADNSSQLKGLNPVDFYLDGKTYKYTYGRSSD
                     LNEANKTLKTVQRKFKDAFIVEFRDGKRVK"
     gene            2437..3339
                     /locus_tag="PLI30_05630"
     CDS             2437..3339
                     /locus_tag="PLI30_05630"
                     /inference="COORDINATES: protein motif:HMM:NF014523.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HOI79070.1"
                     /translation="MQAQGKFSRNFIIGVSFLIALVLLYFGVNFLKGTNILKKQNTYV
                     VMFDDVTGLYPSSPVYVNGYQIGLVNAIKMHSTDPIQFAVDINLEGDYRIPQGSYFEF
                     GSDLLGASTVSLVANKEASKLLSPGDTLHGRQKQGMMNSVANVAPKADSILTHIDSVV
                     LTLNKLMSNPMWEQSIAGIGSTVAQLDESSKSVNAVMASIRKDLPTVTQNLTAVSNDL
                     KEVTAELNSLELQKTFNSIDNTVENLKILSSKLTSTDNSLGKLTNDTQLHDSLTNTIQ
                     TATKLLEDIRENPERYLSIRVRLF"
     gene            3723..5123
                     /gene="dnaA"
                     /locus_tag="PLI30_05635"
     CDS             3723..5123
                     /gene="dnaA"
                     /locus_tag="PLI30_05635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005637083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HOI79071.1"
                     /translation="MNTDYKILWKNCLDVIRDIIPEAAFNTWFSPIVPLKYEDNVLTI
                     QVPSQFFYEYLEDKYIDLIQQTLYRKIGEGTILNYRILVEAESNTMVEMRGEPKFTVS
                     DKKNQNPVSVKVPSPFDRVEPAEFDSQINSKYTFNNFFEGESNRLARTAAEAVGLNPA
                     KTAFNPLFVYGKSGVGKTHLCHAVGSKVLELYPDKKVLYITAHLFKVQYTDARRFNTI
                     NDFINFYQSIDVLIIDDIQELSGLEKTQNTFFHIFNHLHQNNKQLVMTSDCSPTEMQG
                     MEERLLTRFRWGLSTKLERPDKELRKKILKHKILTDGLTISDQVIEFIAENVTENVRD
                     LEGIIVSLMAHSIINNREIDITLARRVMEQNIRFEKKKITVQKIQETVSDFYNVKRDL
                     IQSASRKREIVQARQVTMFFIKKHTELSLSQIGIQVGNRNHATVLHACNTIRDLSEVD
                     KGFRADIEEIERLLQS"
     gene            5153..6016
                     /locus_tag="PLI30_05640"
     CDS             5153..6016
                     /locus_tag="PLI30_05640"
                     /inference="COORDINATES: protein motif:HMM:NF016146.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease"
                     /protein_id="HOI79072.1"
                     /translation="MRLFRFLLYLSLFALCLACKQKPSDSGTNLSTNTYREYYSAATG
                     KKGAELKTALFGIIGNPDIVSYAEIWSAFEKTDVKADGSVWDMYSDNPSGEIIYSFSF
                     HHKKCGTYRSEGDCYNREHTIPQSWFKGASPMYSDLFHIYPTDGYVNNRRGNLPLGEV
                     GIFSWKSANGSRIGQNTSGNFTQTVFEPIDEYKGDFARTYFYMVTAYESNVASWESPQ
                     VGGNTYPAIKNWALEMFLRWHREDPVSTKERDRNQAVFGIQKNRNPFIDHPELAEHVW
                     GNKKNQPFNLK"
     gene            6026..7279
                     /locus_tag="PLI30_05645"
     CDS             6026..7279
                     /locus_tag="PLI30_05645"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HOI79073.1"
                     /translation="MKKYTFFLLLLFFFPSTNVVSQPVRIAILSDIHYLSPEIAQPGA
                     ALEKYEIATGRNLSDLHAVLDKTLAEIEAAGTDILLITGDITNHGEKQSHIDFTKKLY
                     PLQQRGMRIQVIPGNHDINIPDSRAYIGEIPAPAQSISSKEFPEIYGPFGYNGALKLD
                     TASLSYLAEINENTWLLCFDTNRYAEHKTTSVSGGRILPSTMNWALDILRQAKAKNVT
                     VLGMMHHGIVEHMPYQATFFPGHILENWESAAGILAAAGLKIVFTGHFHSNDITQFTG
                     SAENILYDVETGSLSQYPFPYRLITLDSTSLSIDTRFIESVPGTAGLQDKYRKTTESI
                     IRRGVRARLQQTGIPFADDAREALVELLTGLNVLHLKGDEVLDEGMLRSIQQFAEVMD
                     DENFDINSFQLDFPPADNKLKINLK"
     gene            7276..>7808
                     /locus_tag="PLI30_05650"
     CDS             7276..>7808
                     /locus_tag="PLI30_05650"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019540238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HOI79074.1"
                     /translation="MKQHKPLESCLHPGCIEAGCDEAGRGCLAGPVFAAAVVLKPGFR
                     CEELNDSKQLSERERNRLRPIIEQSAMCYAVEMCTPDEIDELNILWASVKAMHKALSR
                     LTVTPEHILIDGNRFHPFQQIPHTCVIKGDGKYLSIAAASVLAKTYRDEYMQNLHNQF
                     PVYGWMHNKGYPTRAHR"
BASE COUNT         2392 a         1799 c         1694 g         1923 t
ORIGIN      
        1 caaaaaaaca gcaaagaatg ggctaactct ctttttttat gtatatttgc accgtttaaa
       61 gaataaaaga aaaatcgtat gaaaaaattt tcactcctat taatcacagc tttcgttttg
      121 atctcttgtc aaaacaacaa aacttacaaa attgacggaa ccgttactga tcctgcctat
      181 gaaggcaaaa acgtatatat tcaggaaatg acaaccaacg agatggtgtc ggttgacact
      241 gccgttgtaa caaacggctc gttcaaattc gaaggatctg ccgactcaac cgtattacgc
      301 tttatcgctc tggacgaaac ggttaaccct cagaaaccat caagggtgct tgccatttta
      361 gagccgggaa ccatccgcgt gcagttcgat tccaccgtta cggtttcagg ttcacagcta
      421 aacgacgcct acaatacgtt caggacagaa caggaaaata taacgaaaga aattcgcaca
      481 ctctctcaac aataccagga agctgcatcg acgggtgcaa tgacagactc actggatgcc
      541 gcacttaccg cgcaatacga aaagttatcc ggcgatatat ctagcaaaac agctgatttc
      601 atcaaagcca acatagccaa tccgctgggt aaattcctct ttatgacttc cgcagagatg
      661 tttgaaccgg aaacccagcg cgaaatcctt gcgttaacgg atgaagcata taaatcgcaa
      721 gaaagtattc aacgcatcgt taaacgtctt gaaaatgccg aaagagtagc tatcggccaa
      781 aaatttctcg attttacaat gaaagatccg gaaggaaacg acatttcatt atcggattac
      841 gcaggaaaag gaaaggttgt cctgatcgac ttctgggcag cctggtgcgg cccttgccgc
      901 gacgaaatgc ccaatgtggt tgcagcttac aacaattaca aaaacaaagg ttttgaagtt
      961 gtgggagttt ctctcgaccg ggaaagagaa agatggctga gcggcataaa agagttgaac
     1021 atgacctggc cccagatgtc tgaacttaag ttctgggata ccccggtggt agagctgtac
     1081 gctttccgcg gtattccgca caccgtcctt ttggacaagg aggggatcat tattgccaaa
     1141 gacctgcgtg gagcagaact tcacaataaa ctgaatgaat tgttgggaga ataaaatccc
     1201 gttatttcac ctgtttaaaa gggctgcacc gttggtgtgg cttttttttg ttaaaaatag
     1261 tgtaatcacg gcttactgcg ctttagtaca aaataattat ctatttttgt acgaaataaa
     1321 actgacagaa agatgctgtt acgactgaaa aaacttatta tttacggttt aggagtttta
     1381 tttctgattc cggcagaaag tgctttcgct caaaaaaacg gattcacggt tgttattgac
     1441 cccggccacg gaggccgcga tccaggtgcg atcggatcaa gctcaaaaga aaaggacatc
     1501 gttctcgttg tcgggaaaaa gctaggcgag ctcatcaaga acaaccatcc ggacgtaaaa
     1561 gttttataca caagagatac cgataggttc gtagagttaa acaaaagggc cgagatagcc
     1621 aacaaagcta aggccgacct gtatatttcg ctacactgta atgcgcttga caggagaaaa
     1681 aggtctccgc aaggcgtgga gacatttgtg ttggggctgc accggagcaa ggacaacctg
     1741 gatgttgcca aagcggaaaa ctcggtaatt ctctatgaag atgattattc caccaagtac
     1801 caggggttta accccaacga gccagagtca tacatcatgt ttgagtttat gaccgaccag
     1861 taccttcagc aaagcgttta ctttgcatcg ttggtacaaa acaggctggt aaacaactcg
     1921 aaaaggacta accgggatgt caggcaggcc ggttttctgg tgctccggga agtggctatg
     1981 ccgagtatcc ttgtggagct ggggtacatt tcaaatcctt ccgacgaaaa gtatatgaaa
     2041 accgaaagcg gacaaagatc attggcaaac tccgtttatt tggcttttaa ggaatacaaa
     2101 cacgaatacg acaaaaaaag tcatgtattt accaatccgg aacaacgaaa gacaattgca
     2161 gccaaccctc cctctaccgg gatggatgta accggaacaa gcgaatacag gatacagttc
     2221 cttacagctc cccgaaaact agccgataat tcttcgcaac tgaaagggtt gaatccggta
     2281 gatttttatt tagacggcaa aacatataaa tacacgtacg gccggtcatc cgatcttaat
     2341 gaagcaaaca aaacactcaa aaccgttcaa agaaaattca aagacgcttt cattgttgaa
     2401 ttcagggacg gcaaaagagt aaaataagaa taatttatgc aagctcaagg caagttcagc
     2461 agaaatttca tcatcggagt ttcctttctt atagccctgg ttctccttta ttttggagtg
     2521 aactttctga agggaacaaa tattttgaaa aagcaaaaca cctacgttgt catgttcgat
     2581 gatgttaccg gactttaccc ctcatcaccg gtttatgtaa acggatacca gatcggactg
     2641 gtcaacgcca tcaaaatgca cagtacagac ccgatccagt tcgctgtgga catcaacctc
     2701 gaaggcgatt atcggattcc gcaggggagt tatttcgaat ttggatcgga tctactggga
     2761 gcctctacgg taagcctggt tgcaaataaa gaggcttcaa agcttctttc tcccggagac
     2821 acgctgcacg gccggcagaa acaagggatg atgaacagcg ttgccaacgt tgcccccaag
     2881 gctgactcca ttctgacaca catagactct gtagtcctga cactgaacaa actgatgtct
     2941 aatccaatgt gggaacaatc gatagcaggt atcggatcta cagttgccca actggatgaa
     3001 tccagcaaaa gcgtgaacgc tgtaatggca tcgatccgaa aagatttacc aacagtaacc
     3061 caaaacctca cagccgtttc caacgattta aaagaggtta cggcagaact taattcgctg
     3121 gaactccaaa agacgttcaa ctcaatcgac aatacggtag aaaacctgaa gatactgtca
     3181 tcaaaactga ccagtaccga caattcattg gggaaattaa ctaacgacac ccagttacac
     3241 gatagtttga caaacaccat acaaactgca acaaaactgc tggaagatat tcgtgaaaat
     3301 cccgagcgat atttgagcat aagggtgcgc ctgttctaag tttcctaaat atggaataaa
     3361 tttgccgatc aaactttata tagaatgatt ctaaatatca gccctgtttt tcgtcgtatg
     3421 ataaaccctt tgggtaattg agtttaagac ataaaacaca tgcttatttt gtacaaacgg
     3481 gtaaagacta tacacttatc acagagttct atatatcaat tgattacgac aatgaattca
     3541 ggcccaaccc gcttataaac agcagcatac gcctatccaa ttgataatta agcgagtgtt
     3601 tttatttcag gaaaaacaaa ggaaacagga tttttttttc tcgatttttt tttgaaaatt
     3661 tgtattcccc aaattaatta aacacaacca atagaagaga ggaaaatcgt taatttattc
     3721 gaatgaacac tgactacaaa attttatgga agaactgtct tgatgtaatt cgtgatatta
     3781 tccccgaagc tgcattcaac acgtggttta gtccaattgt cccgttgaaa tatgaagaca
     3841 atgttctcac cattcaagta cccagtcagt ttttttacga atatcttgag gacaaataca
     3901 ttgatcttat ccagcagacc ctctaccgca aaataggaga aggaactatt ctcaactacc
     3961 gcatcctggt ggaggcggaa agcaatacta tggtggagat gcgcggggag cccaaattca
     4021 ctgtttcaga caagaaaaat caaaatccgg tttctgtaaa ggtacctagt cctttcgaca
     4081 gagttgaacc ggctgagttc gattcccaga taaattccaa atacacgttt aacaacttct
     4141 tcgaaggcga aagcaaccgc ctggcgcgca ccgcagccga agccgtgggt ttaaatccgg
     4201 caaaaaccgc ttttaatccg cttttcgtgt acggaaaatc gggagttgga aaaacgcacc
     4261 tttgtcacgc tgttggatcg aaagtgcttg aactatatcc ggacaagaaa gttctttata
     4321 ttacggcaca cctgtttaag gtacaataca ccgatgcccg gcgtttcaac acgataaacg
     4381 atttcatcaa cttttaccaa agcattgatg tcctcattat tgatgatatt caggaattat
     4441 ccggattgga aaaaacccaa aacacttttt ttcacatctt caaccatttg catcagaaca
     4501 acaagcaact ggtcatgaca tcggattgtt cgcccaccga aatgcaggga atggaagaac
     4561 gtttattaac tcgtttccgt tgggggcttt ccaccaaact ggagcgtccg gacaaagaac
     4621 ttcgcaaaaa aatactgaag cataagatct taaccgacgg actgactatc tccgatcagg
     4681 taatcgaatt tattgctgaa aacgtgacgg aaaatgtgcg cgacctggaa ggcatcattg
     4741 tttcgctaat ggctcactcc atcatcaaca accgtgaaat tgacattact ctggcacgcc
     4801 gcgttatgga gcaaaatatc cgtttcgaga agaaaaagat taccgtgcaa aagatccagg
     4861 aaacggtcag tgatttctac aatgtaaaga gagatttgat tcaatcagcc tcccgaaaaa
     4921 gagaaattgt acaggcgcgt caggtaacca tgtttttcat caagaaacac accgaactgt
     4981 cgctttctca gatcggaatt caggtgggaa accgaaatca cgctactgtc ctacacgctt
     5041 gcaacaccat cagggacctg tctgaagtag acaaaggttt tcgcgccgac attgaggaga
     5101 tagagcgtct gctgcaatct taacattacg ggtatctcac ccacagtata caatgagact
     5161 ttttcgtttt cttctgtatc tgtctctgtt tgccctctgt ctggcatgca aacaaaagcc
     5221 gtcggactcc ggcacaaacc tgtctacaaa cacttaccgg gaatattaca gcgcggctac
     5281 ggggaaaaaa ggggcggaac taaaaacagc acttttcggt ataatcggca atccagatat
     5341 cgtttcgtac gctgagatct ggtcggcttt tgaaaaaacc gacgttaagg ccgacggttc
     5401 tgtctgggat atgtattccg ataatcccag cggggaaata atttactcct tctcgtttca
     5461 tcataagaaa tgtggcactt accgcagcga aggtgattgc tataaccgcg aacacaccat
     5521 tcctcaaagc tggtttaagg gagcatcacc catgtattcc gaccttttcc acatttaccc
     5581 aaccgacgga tacgtgaaca accggcgggg aaatttgccg ctgggtgaag ttggtatttt
     5641 ttcttggaag tcggcaaacg gttcgagaat cggacaaaat acatccggta atttcactca
     5701 aaccgttttt gagccaatag atgagtacaa gggcgatttt gcccgaacct atttctatat
     5761 ggttacggct tatgagagca acgttgccag ctgggaatcg ccacaggttg gaggaaacac
     5821 ctatccggcc attaaaaact gggcacttga aatgtttctg cggtggcatc gcgaagatcc
     5881 ggtcagcacc aaggaacggg atcgcaacca agccgtcttt ggcatccaga aaaaccgaaa
     5941 tccgtttatc gaccatcccg aactggcaga gcatgtgtgg ggaaataaga agaatcaacc
     6001 ttttaatctg aagtagaata ttgccatgaa gaaatacact ttttttttgc tgctcttatt
     6061 ctttttccca tcgacaaatg tcgtttcgca accggtgaga atcgccatcc tcagcgacat
     6121 acattacttg agtccagaga tagctcaacc gggagcagct ctggaaaagt atgaaatagc
     6181 cacgggcaga aacctttccg atttacacgc cgttctcgac aaaactctcg cggagataga
     6241 agctgcggga acagatatat tactcatcac aggagacata actaaccatg gcgagaaaca
     6301 aagccatatc gactttacca aaaagctcta tcctttacaa cagcgtggaa tgcgaatcca
     6361 ggtcatcccc gggaatcacg acatcaacat ccccgactcg agggcatata tcggtgagat
     6421 ccctgctcca gctcaaagca tttcgtccaa agagtttcct gaaatttatg ggcccttcgg
     6481 ctataacggc gcgctaaaac tcgacactgc ctcattaagt tatctggcgg aaattaacga
     6541 aaacacgtgg ttgctttgtt tcgataccaa tcgatatgca gaacataaaa ccacgtcagt
     6601 ttcgggtggg cggattctcc cctcaacgat gaattgggcc cttgatatcc ttcgccaggc
     6661 aaaagcaaaa aacgttactg tcctgggtat gatgcatcac ggcattgtag aacacatgcc
     6721 ttatcaggcc acgttttttc cgggccatat actggaaaac tgggagtctg ccgcaggaat
     6781 ccttgccgct gccggcttga aaatcgtatt taccggacac ttccactcca acgatattac
     6841 acagttcacg ggttctgcag aaaacatctt gtacgatgta gaaaccggaa gcctgtcaca
     6901 gtatccgttc ccttatcgac tgataacact cgactccacg tctctctcca tcgatacccg
     6961 ctttatcgaa tctgttcccg ggacagccgg tttgcaggat aaataccgca aaacaacgga
     7021 atcgatcatt cgtcgaggcg tccgggcacg acttcaacaa acgggaatac ctttcgcgga
     7081 tgatgcccgg gaagcgttgg tcgaactgct cactggctta aatgtattgc acctgaaggg
     7141 cgatgaagtt cttgatgagg gcatgctccg ctcaatccaa caatttgctg aagtaatgga
     7201 tgatgagaat ttcgacataa attcttttca gctcgatttc cccccggcag acaacaaact
     7261 gaaaattaat cttaaatgaa gcaacacaaa ccgttggaga gctgccttca ccccggttgc
     7321 atcgaagccg gttgcgacga agcgggacgc ggatgccttg ccggccctgt cttcgcagct
     7381 gcagttgtcc tcaagcccgg attccggtgt gaagagctca acgattccaa gcaattatcg
     7441 gaaagggaac ggaaccgtct ccgacctatc attgagcaga gtgcgatgtg ctacgccgtt
     7501 gaaatgtgca ccccggatga aattgacgag ttaaatatcc tctgggcatc ggttaaagcc
     7561 atgcacaaag ccctaagcag gctgaccgtg acgcctgaac atatcctgat agacgggaac
     7621 cggtttcatc cgttccaaca gattccacac acatgtgtga taaagggtga cggaaagtac
     7681 ttgtcgattg cggcagcatc ggtgctggca aaaacatacc gggatgagta tatgcaaaac
     7741 ctacacaatc aatttcccgt ttacggatgg atgcacaaca aaggataccc tacacgtgcg
     7801 catcgcga
//
LOCUS       DAOSDR010000107         7793 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrimonas sp. isolate AMR_MDS_1826
            NODE_3452_length_7793_cov_3.924916, whole genome shotgun sequence.
ACCESSION   DAOSDR010000107 DAOSDR010000000
VERSION     DAOSDR010000107.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863355
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrimonas sp. (wastewater metagenome)
  ORGANISM  Petrimonas sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Dysgonomonadaceae; Petrimonas.
REFERENCE   1  (bases 1 to 7793)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7793)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:10:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,720
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 35
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7793
                     /organism="Petrimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3452_length_7793_cov_3.924916"
                     /isolate="AMR_MDS_1826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2023866"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..197
                     /locus_tag="PLI30_05655"
     CDS             <1..197
                     /locus_tag="PLI30_05655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005785715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="flotillin-like FloA family protein"
                     /protein_id="HOI79075.1"
                     /translation="AKAQEARAKVIEAEAEVPKAMAEAFRSGNMGIMDFYRMKNIQSD
                     TDMRDAIAKPQGGAKEESSK"
     gene            238..1104
                     /locus_tag="PLI30_05660"
     CDS             238..1104
                     /locus_tag="PLI30_05660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011966697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside phosphorylase"
                     /protein_id="HOI79076.1"
                     /translation="MRIIPNSEFIVNPDGSVFHLHIRPEQLSDKIVMMGDPDRVTLTA
                     SFFDSIECDVRSREFHTVTGMYKGKRLTALSHGIGTDNIDIVVTELDALANIDFHTRT
                     VKPEFRQLTMIRVGTSGGMQPHCPIGSYVVSEKSIGFDGLLHYYAHSQDVREEDFEEA
                     FQKQVNWSPYHCSPYVVSADEELVERIGYDMIRGVTISAIGFYGPQGRHVRLPLADPE
                     LNAKIESFRYSHYSITNYEMESSAIAGLGKMMGHKCMTVCAIIANRVALESNADYKGS
                     IEDLIKVVLERI"
     gene            1149..3947
                     /locus_tag="PLI30_05665"
     CDS             1149..3947
                     /locus_tag="PLI30_05665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009860342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HOI79077.1"
                     /translation="MKKILLLSLVLLGITATAQQNPPQPIDPNVRKGILENGLTYYIR
                     QNKLPENRADFYIAQKVGSMQEEDNQSGLAHFLEHMAFNGTKNYPGKDMLNYLQDNGI
                     KFGTNINAYTSLDETVYFLTDVPTTHENLVDSVLLVLHDWSNGILLEDEELEKERGVI
                     REEWRTGGGAQFRLWKKMLPVMYKDSKYANRLPIGSIDVINNFKPEEIRAYYKKWYRP
                     DLQGIIVVGDFDLDNMETKVKELFNKIPAQENPATREYFPVPDNDTPIVSIATDPEAT
                     RTQLMVFYKHEPIPNEIKLSQAGLVLNYIKSAAATMINERFQEMVQKPNAPFTSAYAY
                     DDEYFVAKTKDAWTVVGGSADDKIKDAIAAMIRETERVRRFGFTASEYERARTNILKQ
                     YENGYNNRDKERNSRYSQEYVSSFINSEPIPGIEYEYNMMNMIAPNIPVEAINQTIAQ
                     LIGDKNMVISVSGPEKEGLVYPTEDELVAAINNVKSEKIEAYVEEVSNEPLIATPPTP
                     GKIALVEKNEALDATVWTLQNGMKVILKTTDFKDDQIVMTGSSTGGYSQYAVQDPINA
                     RMMNNIITLGGVGNFSATHLTKALAGKTASARPGVSLTTQDFNGSSSIKDFETMLQLV
                     YLYFTAPRSDDDAFQSFIQRMETQLKNQEAEPMVAFSDAATKALYGDNPLTKRTKVED
                     LAGIDYKRIMEMYKQLFRNPGSFVFTFVGNIDEAKVKPVIEQYLASLPGKAVKGEFIH
                     VPMDYLKGKSENIFQREMQNPKASVFIASTGNMERSQKNTLLMSIFDQILDIVYTEKI
                     REDEGGTYGVYTQGGISRYPKGQSTLQIVYDTDPTKMENLNAIIHRELKSIADNGPRA
                     EDFSKVKEYMLKQYNENLKENSYWMNVLDTNYFYGEDNHSNYLTILNSITANDVKNFV
                     KEFLSQGNEAVVVMLPKE"
     gene            complement(4009..6219)
                     /locus_tag="PLI30_05670"
     CDS             complement(4009..6219)
                     /locus_tag="PLI30_05670"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005657656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HOI79078.1"
                     /translation="MATNENQSIQKNFPVLQMTCAGCAAGVENKLKSLKGVKNAAVNF
                     ATATALVDFQPGEIDAEGIQKAVQSIGYDLVLEEENQQDLLDEIHEKNYRQLKNKTVW
                     ALILSLPVVIVGMFLMNIPYADLIMWAFATPVVLWLGRDFFITAWKQTRHRTANMDTL
                     VALSTGTAYVFSLFNMLFPEFWQSKGLEGHVYFEAAAVIIAFILLGRLLEEKAKGSTS
                     SALKKLMGLQPKTVTVIRDDNNQVEIAIEKLAVGEIVQVKPGEKIAVDGTVISGDSFV
                     DESMLSGEPMSVRKVKGEKVFAGTINQKGSFRFRADKVGKETMLAHIIKMVQDAQGSK
                     APVQKLVDKIAGIFVPVVIGIAIITFIVWLIFGGDNALTHALLAFVTVLVIACPCALG
                     LATPTAIMVGVGKGAEKGILIKDAESLELSQKVNAVVLDKTGTITEGKPQVTDELWMD
                     ESAKALLMALEQQSEHPLADAVVAHLGNGKEILVSGFESLTGRGVKGIVNDKMYFAGN
                     RKLMSENNIYISQELQANSDEWGQLAKTIIWFADNKKALAVLAISDKIKETSVTAIRK
                     MQEMGIEMYMLTGDNVATSKAIAGQTGIRHYKAEVLPQEKSEFVKELQQQEKVVAMVG
                     DGINDSAALAQADVSIAMGQGSDIAMDVAKMTIISSDLTKIPQAIRLSRQTVQTIRQN
                     LFWAFIYNIIGIPIAAGILYPVNGFLLNPMIAGAAMALSSVSVVTNSLRLKWKK"
     gene            complement(6304..6804)
                     /locus_tag="PLI30_05675"
     CDS             complement(6304..6804)
                     /locus_tag="PLI30_05675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019540133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HOI79079.1"
                     /translation="MEKLGFNPKDVVLGEVLLEEELSDEQKEKVRAAIEPLGFELIDD
                     RKSRIIEKIKNVIIDLVHRKNSHLQTNLSDMLSEELNHDYSYLSNLFSEVEGTTIEKY
                     FIAQKIEKVKELLVYDELSLSEIAYRLNYSSVAYLSNQFKRVTGLTPGHFKQIREIKR
                     KPLDEV"
     gene            complement(6878..>7793)
                     /locus_tag="PLI30_05680"
     CDS             complement(6878..>7793)
                     /locus_tag="PLI30_05680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004329933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOI79080.1"
                     /translation="ETVKSFPPIRVWGTVGFIVSMIAVDLIRIDGFQLSKTSYQLYFS
                     AILSLVLGIYAFTLPNCPVNKESENRSLIDKLGLRAFALFKHRQMAVFFLFSMCLGIA
                     LQITNAFANDYLTNYFGNIPKFSGTFGVEHANILISLSQMSETLCILLIPFFLKRYGI
                     KTVMLISMFAWVLRFGLLGAGDPGGGVWMLILSMLIYGVAFDFFNISGSLYVEKVTRP
                     AIRSSAQGVFMIMTNGFGAFIGSYAAGKVVDLVGWPNSWYVFAGYSLLVAFLFMVFFQ
                     YKHESETGRQGDLATGKEDGDGDGVILN"
BASE COUNT         2306 a         1847 c         1629 g         2011 t
ORIGIN      
        1 aagccaaggc ccaggaagcc cgtgcgaagg taattgaagc cgaagctgaa gtacccaagg
       61 caatggccga agcgttccgc agtggcaata tgggcattat ggatttctac cggatgaaga
      121 acatccagtc ggataccgat atgcgcgatg cgattgccaa gcctcaaggc ggagcgaaag
      181 aagagagttc gaaataaata gagaacgaaa gtaaagaagt cttaaaccat tacttttatg
      241 agaataatcc ctaattcgga atttattgtc aatcccgatg gcagtgtatt tcatcttcac
      301 atcaggcccg agcaactgtc ggacaagatc gtgatgatgg gtgaccctga ccgcgtaaca
      361 ctgaccgctt cctttttcga cagtatcgag tgcgatgtgc gcagcaggga attccacacg
      421 gttacaggca tgtacaaggg gaaacgtctt accgcactct cgcacggcat cggaaccgat
      481 aatattgata ttgttgtaac agagctggat gctttggcca atattgattt tcatacccga
      541 acggtaaaac cggagtttag gcaattgacc atgatccgcg tgggaacatc gggcggcatg
      601 cagccgcact gccctatcgg atcgtatgtg gtctccgaaa aatcgatcgg ttttgacggg
      661 ttgcttcact attatgccca ttcgcaggat gtacgggaag aagattttga agaagccttt
      721 cagaaacagg taaactggtc accctaccac tgttcgccct atgtggtgag tgccgacgaa
      781 gagctggtgg aacgcatcgg atacgacatg attcgcggag taaccatttc cgccatcggc
      841 ttctatggcc cacaaggcag acacgtgagg ctgccccttg ctgatcctga gttgaatgca
      901 aaaatcgagt cgttccgtta cagccattac tccatcacca attacgagat ggaaagttcg
      961 gccatcgcag gcttgggtaa aatgatgggc cacaaatgca tgaccgtttg cgccatcatt
     1021 gccaaccggg tagccctgga atcgaatgct gactacaaag gatcgatcga ggatctaata
     1081 aaagttgtac tggaaaggat ataactatct acgattgaac aaaccaccta tttaattaac
     1141 aatcaacaat gaaaaaaatt ttattattgt cgttggtgtt gctggggata acagcaacgg
     1201 cgcaacagaa tccacctcag cctatcgatc cgaatgttcg gaaaggcata ttggaaaacg
     1261 ggttaactta ctacatccgt cagaataaac tacctgaaaa ccgggccgat ttctacatcg
     1321 ctcaaaaagt agggtcaatg caggaagaag acaatcagtc gggactggcg cattttctgg
     1381 agcacatggc atttaacgga acaaaaaatt atccgggcaa ggatatgctc aattacctac
     1441 aagataacgg aattaaattc ggaacaaaca tcaacgcata tacctcgctt gatgaaaccg
     1501 tctatttttt gaccgacgtc cccaccaccc atgaaaacct ggtggactcg gtgcttctcg
     1561 tccttcacga ctggtcgaat ggcattttgc tggaggacga agagttggaa aaagaacgcg
     1621 gtgttattcg tgaagaatgg cgtaccggcg gcggggcaca attccggttg tggaaaaaga
     1681 tgcttcccgt aatgtataaa gacagcaaat acgccaatcg cttgcctatt gggagtattg
     1741 atgttatcaa caacttcaag cccgaagaaa tccgcgcgta ctacaaaaag tggtaccgcc
     1801 ccgatttaca gggaatcatc gttgtgggtg atttcgacct cgacaatatg gaaaccaaag
     1861 taaaggaact tttcaacaag ataccggctc aggaaaatcc cgctacccgc gaatattttc
     1921 ctgtccccga taacgatacg cccatcgttt ccatcgccac cgacccggaa gcgacacgta
     1981 cacaactgat ggttttttac aaacacgaac ccatacccaa cgaaattaaa ctgtcgcagg
     2041 ccggcctggt tttaaattac atcaaatcgg ctgcagctac catgatcaat gagcggttcc
     2101 aggagatggt tcaaaaaccg aatgctccgt ttacctctgc ttatgcttac gacgacgaat
     2161 attttgtagc caagaccaaa gatgcctgga cggttgttgg aggctctgcg gatgacaaga
     2221 tcaaagacgc aatagccgcc atgattcgcg aaacggaacg tgtgagacgc ttcggtttca
     2281 ccgcttcgga atatgaacgt gcccgtacca atatcctgaa acaatacgaa aacggatata
     2341 acaaccgcga taaagagagg aacagccgtt attcgcaaga gtacgtaagt agcttcatca
     2401 acagcgaacc tatccccggt attgagtacg agtacaacat gatgaacatg atagctccca
     2461 acattccggt tgaggctatc aaccaaacta tcgcgcagct tatcggcgat aaaaacatgg
     2521 tcatctctgt atcaggtcct gaaaaggaag ggttggttta tccaacggag gacgaacttg
     2581 ttgcggcaat caacaacgtg aagagtgaaa agattgaagc ctacgtagaa gaagtatcca
     2641 acgaaccgct tatcgcgacg cctcccacac ccggaaagat cgcactagtg gaaaaaaatg
     2701 aagcgctgga cgcaacggtc tggacgcttc agaatggcat gaaagtaatc ctgaaaacta
     2761 ccgattttaa ggacgaccag attgtcatga ccggatccag taccggcgga tattcacaat
     2821 atgccgtaca ggatccgatc aacgcaagaa tgatgaacaa catcatcacg ttgggggggg
     2881 taggtaattt cagtgccact catctgacaa aagcgcttgc cggaaaaacg gcatcggccc
     2941 ggccaggtgt ttcgcttacc acacaagatt tcaacggttc gtcttccata aaggattttg
     3001 agaccatgct tcaactggta tatctctatt tcacagctcc ccgaagcgac gacgatgcct
     3061 tccaatcgtt tattcaacgc atggaaacac agttgaaaaa tcaggaagcc gaaccgatgg
     3121 tggccttcag tgatgcagcg acgaaagctc tttatggcga caatccgctg acaaaacgca
     3181 ccaaagtgga ggacttggct gggatagatt acaaacgcat catggaaatg tacaaacaac
     3241 tgttccgtaa tcccggcagt tttgttttca cgtttgtcgg taatatcgat gaggcaaagg
     3301 tgaaacctgt tatcgagcaa tacctcgctt cgcttccggg aaaagccgtg aaaggtgaat
     3361 ttatccatgt accgatggat tacctgaaag gaaagtcgga aaacatcttc caacgtgaaa
     3421 tgcaaaatcc caaagcatcg gtattcattg cttccacagg aaacatggaa cgtagccaga
     3481 agaataccct gctgatgagt atttttgacc agatccttga catcgtttac accgaaaaaa
     3541 tccgtgaaga tgaaggagga acttatggcg tttatacaca aggcggcatt tcacgctatc
     3601 cgaagggtca aagtacattg cagattgttt acgatacgga cccgaccaaa atggaaaacc
     3661 tgaatgccat catccatcgc gaactgaaat caatagccga caacggtccc cgcgccgagg
     3721 atttcagcaa agtgaaagaa tatatgttga agcagtacaa cgagaacctg aaggaaaaca
     3781 gttactggat gaacgtgctc gacaccaact atttttatgg agaagacaat cactccaatt
     3841 atttaacgat attgaattcc attacagcaa acgacgtgaa aaactttgtg aaagaattcc
     3901 tgtcgcaagg caacgaggct gttgtggtga tgttaccgaa ggaataatcg ggacacgaaa
     3961 gctttgaact ttatatgtgt agagacggtg tcgcgcaccg tctctacatt attttttcca
     4021 cttcaacctt aaactgttgg tcacaacgct cacgctgctc agtgccatgg ctgctccggc
     4081 aatcatcgga ttaagcaaaa aaccgtttac gggatagagt atccccgcag caatgggaat
     4141 gccgataatg ttatagataa atgcccagaa caggttctga cgaatagttt gaacggtttg
     4201 gcgggataat cggatggcct gcggaatttt agtcaaatca gacgaaataa tggtcatttt
     4261 tgccacatcc atggcaatgt cgctgccctg ccccatagca atgctcacat cagcctgtgc
     4321 cagagcggcg ctgtcgttga tgccgtcgcc caccatagcc acaacttttt cctgttgttg
     4381 caactctttt acaaattccg atttttcctg cggtaacacc tccgctttgt agtgcctgat
     4441 tccggtttgc ccggcgatgg ctttggatgt tgctacgtta tcgcctgtga gcatatacat
     4501 ttcgataccc atttcctgca tctttcggat agccgtaact gatgtctcct taattttatc
     4561 ggaaattgcc agaacagcca aggctttttt gttatcggca aaccaaatga tggttttagc
     4621 caattggccc cactcatcgg aattagcctg taactcctgc gaaatataga tattattttc
     4681 cgacatcagt tttcggtttc ccgcaaaata cattttatca ttaacaatac cttttactcc
     4741 tcttcccgta agactttcga agccggaaac caaaatctcc ttaccatttc ccaaatgcgc
     4801 aacaacagca tcagccagcg gatgttctga ttgctgttcc aaagccataa gcaaagcttt
     4861 tgcagactca tccatccaga gttcatctgt aacctgcggt ttaccttcgg taatggttcc
     4921 ggttttatcc aatacaacgg cattcacttt ttgtgacaat tccagacttt cagcatcttt
     4981 tatcagtata cccttttcgg caccttttcc caccccaacc ataatagcgg ttggtgttgc
     5041 cagccccagc gcacaaggac aggcaataac cagtacagta acaaaagcca gtagcgcgtg
     5101 tgtgagcgca ttgtctcctc caaaaattaa ccaaacgata aacgtaatga tcgcgattcc
     5161 tataactacc ggcacaaata tacctgcaat tttgtctacc agtttctgta ccggagcttt
     5221 gctgccttgt gcgtcttgca ccattttaat aatgtgtgcc aacattgttt ctttacctac
     5281 tttatcggcc ctaaacctga aactgccctt ttgattgatc gttccggcaa acactttttc
     5341 tccttttacc ttacgcacgg acatcggctc tccgcttaac atactttcgt caacaaacga
     5401 gtcacccgaa attaccgtgc cgtccacggc aattttttct cccggtttca cctgcacgat
     5461 ttcaccaact gccaactttt cgatagctat ctcaacctga ttattatcgt cacgaataac
     5521 agtgaccgtt ttcggctgta aacccatcag tttttttaat gcagacgaag tactgccttt
     5581 ggctttttct tccaacagcc gccccaacaa aataaaagcg ataattaccg ctgcggcttc
     5641 aaaataaaca tgaccttcca atcctttgct ctgccagaat tcgggaaaaa gcatattgaa
     5701 gagactgaaa acgtacgctg tccccgtact taatgccaca agtgtatcca tattagcagt
     5761 acgatgcctg gtctgcttcc aagcagtaat gaaaaaatct cttcccagcc acaaaaccac
     5821 cggtgttgca aaagcccaca tgataaggtc agcatatgga atgttcatca aaaacatccc
     5881 tacaatcaca accggtaggg ataatatcaa tgcccagaca gttttgtttt ttagttgtct
     5941 ataattcttt tcatgaattt catcgagcag atcctgttga ttttcttctt cgagaaccaa
     6001 gtcgtaaccg atggattgaa cggctttttg aataccttca gcatctatct caccgggttg
     6061 aaaatctact agtgcagttg ctgtagcaaa attcacggca gcatttttca cacccttcag
     6121 tgatttgagt ttgttttcta cgcctgcagc acaaccggca caggtcattt gtagaacggg
     6181 aaaattcttt tgtatagatt gattttcgtt tgttgccata accatttctt tttaagtaga
     6241 atgcaaaatt agcaacaaaa gttcaataga gtattgcagt ttttttgaat agatttgtaa
     6301 gatttacact tcgtcgagcg gtttcctttt tatttctcta atttgtttaa aatggccggg
     6361 ggtaaggcct gtcactcttt taaactgatt gctcaggtaa gcaacacttg aataattgag
     6421 tcgataagcg atctcactga gtgatagttc gtcgtaaacc agcaattctt ttaccttttc
     6481 gattttttgt gcgataaaat atttttcgat ggtggtgcct tccacttcag agaaaagatt
     6541 ggacaggtag ctgtaatcgt gattaagttc ttcactcaac atgtcggaaa gatttgtctg
     6601 cagatgactg tttttgcgat gcaccaaatc gataataaca ttttttattt tctcaataat
     6661 acggcttttc cggtcatcga tcagttcaaa acccaacggt tcaattgctg cacgaacttt
     6721 ctctttctgc tcgtcagaca attcttcttc aagcaaaact tcacccaaca caacgtcttt
     6781 cgggttgaaa cccaattttt ccatttcgtt gtgaactacc atcacacaac gattacaaac
     6841 catatttttt acatatagtt tcataaattt ctgaagttta gtttagaatc acaccatcac
     6901 catcaccatc ctctttccct gtcgccaagt caccctgtcg ccctgtctca ctttcgtgtt
     6961 tgtattgaaa gaaaaccatg aacaaaaatg ctaccaatag tgaatacccg gcaaaaacat
     7021 accacgagtt cggccaaccg accaaatcaa ccacctttcc tgcagcatat gaaccgatga
     7081 aagctccgaa accgttggtc ataatcataa aaactccctg agcactcgat cggatggcag
     7141 gccgcgtaac tttttcaaca tatagtgagc ccgaaatatt gaaaaaatcg aatgccaccc
     7201 cataaatcag catagaaaga atcagcatcc aaacaccgcc tccgggatca ccggctccca
     7261 ataatccaaa tctcaacacc caggcaaaca tacttatcag cataacggtt ttaatgccgt
     7321 aacgtttcaa aaagaatggg ataagcaaga tgcacaaagt ttcagacatt tgcgaaagtg
     7381 agatgagaat attggcatgt tccactccga aagttcctga aaatttggga atgttgccga
     7441 aataattggt aagataatcg ttggcgaaag cattggtgat ctgcaacgcg atacccaaac
     7501 acatggaaaa caggaagaaa actgccatct gtcggtgctt gaaaagtgca aaagcacgta
     7561 atcccagttt atcgatcaaa gacctatttt cagattcttt gtttacagga caattgggaa
     7621 gcgtaaaggc gtaaattcca agcacaagcg agagtattgc tgaaaaataa agctgatacg
     7681 atgttttgga taattgaaag ccatctatgc gaattaaatc cacagcaatc atcgatacta
     7741 taaaacccac agttccccaa acacgaatag gcgggaacga cttcaccgtt tcc
//
LOCUS       DAOSDR010000108         7792 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrimonas sp. isolate AMR_MDS_1826
            NODE_3454_length_7792_cov_3.829391, whole genome shotgun sequence.
ACCESSION   DAOSDR010000108 DAOSDR010000000
VERSION     DAOSDR010000108.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863355
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrimonas sp. (wastewater metagenome)
  ORGANISM  Petrimonas sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Dysgonomonadaceae; Petrimonas.
REFERENCE   1  (bases 1 to 7792)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7792)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:10:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,720
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 35
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7792
                     /organism="Petrimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3454_length_7792_cov_3.829391"
                     /isolate="AMR_MDS_1826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2023866"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1797)
                     /locus_tag="PLI30_05685"
     CDS             complement(<1..1797)
                     /locus_tag="PLI30_05685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005649602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HOI79081.1"
                     /translation="MVKILCGLSLSLFLLLFTSCKQNNTETILQQSKSLESGFISPPD
                     TVQTSVYWYWISDNISREGVIKDLHAMKEVGINRAFIGNIGLNDLPSGNVKMFTEEWW
                     EIMHLALKTASDLGIEIGIFNSPGWSQSGGPWIKPEESMRYLTSSQLTVEGPKKLSQK
                     IDPPADFFQDVKVLAYRMPGNVQPDVNDKNATISSVPSHQQLSRIVDKNGATSLSISP
                     ASEFTITFDTGQPFTARSVTVTLAETPASVEAVFQAKDDRGEYRIVSEFSVNRSNISL
                     NVGFKPFAPVVISIPATTSQSFKLVLKNIRKPLNLAEVEISGLPKVERYSEKTLAKMH
                     PTPLPYWDAYLWREQPEADDRSLAVQTDQVIDISESLSTDGTLNWEVPEGNWRILRMG
                     MTTTRVTNSPASPEATGLEVDKMSKKWVAEHFDRFIGEIIRRVPEQDRKTFKVVVQDS
                     YETGGQNFTDGFLDEFRERYGYNPVPFLPVFEGVVVNSQLESDRFLWDIRRMIADKVA
                     YDYVGGLRDVSHKHGLRTWLENYGHWGFPGEFLMYGGQSDEIGGEFWSEGELGDIENR
                     AATSAGHIYGKNKISAESNTSGGPAYSRYPAKM"
     gene            complement(1820..3475)
                     /locus_tag="PLI30_05690"
     CDS             complement(1820..3475)
                     /locus_tag="PLI30_05690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005638207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-rhamnosidase C-terminal
                     domain-containing protein"
                     /protein_id="HOI79082.1"
                     /translation="MTQSYVTPAKLVWSSDQTGEHVKNPEVLLKEFDGQLSTSGAGMC
                     VMRSDTDKQASVLLDFGTELYGGLQLAAGIRGNKKPLKVRVRLGESVSEAMSNSIDNS
                     VPGMNSATNDHSLRDFIIELPWLGSVEVGNSGFRFVRIDLLDLNEDLPLKAVRAIFRY
                     RDIPYTGSFRCDNERLNRIWETGAYTVHLNMQEYLWDGIKRDRLVWVGDMHPEVMTIN
                     TVFGKNEVVRKSLDFARDTTPLPGWMNTISSYSLWWIIIHRDLYLHQGDLAYLKEQQT
                     YLNALIQQIVARVSNGKEELDGGRFLDWPTSEKKDVIHSGLQALTIMSLKAGEDIAGW
                     LKDEALKKMCLSSLSSMQKYIPSHRDSKQAAALLSIANVLPSASASKVILKDGATDFA
                     TFYGYYMLEALAKDGKYEEAMDIISDYWGAMLDLGATTFWENFIYEERKNAGRIDELV
                     SESKYDIHADGGDYCYVGLRGSLCHGWASGPTTWLTEHVLGIKVVEPGAKTIRIKPNL
                     GNLKFAEGNYPTPYGKVYVKHVKQADGKINTTVHAPKEINVIK"
     gene            complement(3605..5479)
                     /locus_tag="PLI30_05695"
     CDS             complement(3605..5479)
                     /locus_tag="PLI30_05695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009229645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 127 protein"
                     /protein_id="HOI79083.1"
                     /translation="MKYYLLLILFFSSFHLFPQKKVKSAVQDKLSPATTANIEGHIGE
                     KLDASFNNRVLAQDAHRLIRPFTIHDEHSCWQSEFWGKWFTSAILAYSYRPDQQLINK
                     LKNAAYELMATQTPEGYIGNYASESRLKAWDIWGRKYVMLGLIAYYDVSKDRKALEAA
                     AKEADFLIQELADKNALIVKMGNHRGMAASSVLEPVCQLYMRTGNERYLDFAEEIVRQ
                     WETPEGPQLISKASVPVGQRFAKPAENWYGWEQGQKAYEMMSCYEGLLELYRITGKES
                     YKKAAEQTWQSITDTEINIAGSGAAMEAWFSGKKIQHVPIAHYQETCVTVTWIKFNQQ
                     LLRLTGESKYADAIEWAYYNALLGAMRPDGSDWAKYTPLSGQRLPGSEQCGMGLNCCN
                     ASGPRGLFTIPLTAVMSSNEGLNINFYMEGSYQTTSPQKNVITVKQHTDYPVSGNVNI
                     SLEMKTAENMTLALRIPSWSDESTVLINDQPVEKVTRGDYLKISRTWKEGDKIQIRLD
                     MKGELHYTGQSPVNVAITRGPVVLTRDERLAGPKLEAVIAPIKDKNGFIHLTPQKNHN
                     SDAWMVFSAKFLPEAYTEYNAEPVEVNLCDYASAGNTMGTYPFFKVWMPQLVDPRKTE
                     "
     gene            complement(5642..>7792)
                     /locus_tag="PLI30_05700"
     CDS             complement(5642..>7792)
                     /locus_tag="PLI30_05700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007838189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 78 protein"
                     /protein_id="HOI79084.1"
                     /translation="AQKRIEDAIIYISGLGHYELSLNGKKIGNDQYNPLWSDYDKTVY
                     YNAYDLTEGVKKGDNTVGVLLGNGFYNEQGGRYKKMQVSFGPPTLFLKISITYTDGTK
                     EEIISDENWKYSPSPIVFNSMYGGEDYDARLEQPGWDTPGFDDSQWLPVVVDNAPNGE
                     LKPQTSTPVREMEYFSIKESMKTGESYVLDMGQNLSGYPAFTVKGKRGDKIRLTVAER
                     INDDGSINQTQSGGPYYYEYTLKGESEETWQPRFSYYGFRYIQVDGAKLSESEDNRDI
                     PVIKAIKSCFVYNSAEPAGSFHSSNEIFNNAHNLIVNAIKSNMQAVFTDCPHREKLGW
                     LEEVHLNGPGLYYNFNLARFAPKIMQDIRDAQLPNGLVTSIAPEYVVFNGGFRDSPEW
                     GSASVIFPFMYYEFYGDNSLIAEHYDVMKRYVDYLTTTAKDHIVSHGLGDWCDYREDQ
                     PYGVSHNTPISLSATTHYFMVIDYLVQAAEMLSKKEDITFYSNLREEVRTAFNKEFFN
                     EDTKQYGTGSQASNAMPLFAGLVNPQDKQSVLDNLVKDIEEKGYRLSTGDVGNRYLFQ
                     ALARNGLNEVMYKMHNHNEVPGYGFQLQFGATTLTELWDPRKGASWNHFMMGQIEEWF
                     YRSLAGIDVEENAYTGFKNFKIAPQVVGDLTYVNASYNTLYGTIKVDWKVDNGQFEMD
                     IEVPINCEAKVYLPQETNYTAVKSGKYSFKKRLQ"
BASE COUNT         1843 a         1868 c         1701 g         2380 t
ORIGIN      
        1 cattttggcg ggataacgcg aataagccgg cccacccgag gtgtttgatt cggctgatat
       61 tttgtttttt ccgtaaatgt gtccggccga ggttgcggct ctgttttcga tatctcccaa
      121 ctctccctcg ctccagaact caccgccgat ctcatcggat tgtcctccat acatcagaaa
      181 ttccccggga aatccccagt gtccgtagtt ctcgagccat gttcttagtc cgtgcttgtg
      241 gctgacatcc cttaatccgc ccacataatc gtacgccact ttgtctgcaa tcatcctcct
      301 gatatcccac aggaacctgt ccgattcgag ttggctgttt actaccactc cttcgaatac
      361 gggtaggaaa ggtacagggt tgtacccgta tctctccctg aactcatcga ggaatccatc
      421 ggtaaagttt tgtcctcctg tttcgtaact atcttgtact actactttga atgttttcct
      481 gtcctgttcg ggtacccttc ttatgatttc tcctataaac ctgtcgaaat gttcagctac
      541 ccactttttg ctcattttgt ccacctccag gccggtcgct tcaggagaag ccgggctgtt
      601 tgtcacacgg gttgtcgtca tccccattcg cagtattctc cagtttcctt caggtacctc
      661 ccagtttagg gttccatctg tagaaagaga ttccgaaata tcgattacct ggtctgtttg
      721 aactgccagg cttctgtcat ccgcttcggg ttgttcccgc caaagataag catcccaata
      781 aggcagaggg gtagggtgca tcttggccaa tgtcttttcg ctgtatcttt ccaccttagg
      841 caggccgctg atttccacct cggccaggtt caaaggcttg cgtatgtttt taagcaccag
      901 tttaaagctt tgtgatgttg tagccggaat ggaaatcacg actggggcga aaggtttgaa
      961 gcccacgttc aggcttatgt tcgatcggtt gacactgaat tccgaaacaa tcctgtattc
     1021 ccccctgtcg tccttagcct gaaagaccgc ttctacggaa gccggtgttt cggccaatgt
     1081 aacggttacg cttcttgccg taaaaggctg cccggtatca aacgtaatag taaattcact
     1141 tgccggagaa attgataggg aagtagctcc gtttttgtca acaatcctcg acaactgttg
     1201 gtgggaagga actgagctta tcgtcgcgtt cttgtcgttt acatccggtt gaacgttacc
     1261 gggcatccgg tatgccaata ctttaacatc ctgaaaaaaa tcggcaggcg gatcaatttt
     1321 ttgcgataat ttttttggcc cttccaccgt taactgggaa gatgtaagat accgcatgga
     1381 ttcttcgggt tttatccacg gcccacccga ctggctccac ccgggggagt tgaatattcc
     1441 tatttcgata cctaagtcag aggctgtctt taaagccaaa tgcataattt cccaccattc
     1501 ttcagtaaac attttaacgt ttccagaagg gaggtcattg agtccgatgt tgccaataaa
     1561 agccctgttt atccccactt ctttcatggc atggaggtct ttaataaccc cttccctgga
     1621 gatgttatcg gagatccagt accagtaaac actggtttga accgtgtcgg gtggggaaat
     1681 aaaaccggat tccagggatt ttgattgttg gagaatggtc tcggtattat tctgtttaca
     1741 cgaagtgaaa agtaatagaa agagagagag agagaggcca cataaaatct ttaccatgat
     1801 gttttgctta aatataaatc tacttgataa cgtttatttc tttcggtgca tgaacggtgg
     1861 tatttatctt tccgtcagcc tgctttacat gtttaacata aacctttccg tatggggtag
     1921 ggtagttgcc ttcggcaaat ttcaggttcc ccaggttggg ttttatccgg attgttttgg
     1981 cgccgggttc tacaacctta atgcctaaaa cgtgttccgt caaccaggta gtgggtcctg
     2041 aagcccaacc gtggcacagg cttcccctca gccctacgta acagtagtcg ccgccatctg
     2101 cgtgtatgtc gtattttgat tcggaaacca attcatcaat ccggcctgca ttttttcttt
     2161 cttcatagat gaaattctcc caaaaagtag tagctcccag gtccagcata gcaccccaat
     2221 agtccgagat gatgtccatg gcttcttcgt attttccgtc tttagccaat gcttccagca
     2281 tgtaatatcc gtagaaagtg gcaaaatcgg ttgccccgtc cttcaatatc acttttgatg
     2341 cagaagcgga agggagaacg ttggcgatgg acagcagggc ggctgcctgt ttgctgtccc
     2401 ggtgtgaagg gatgtatttc tgcatggagg ataagctgga taggcacatc ttttttagcg
     2461 cctcatcctt taaccagcca gcaatatctt ctcccgcttt caggctcatg atcgtcaggg
     2521 cttgcaaccc ggaatgaata acatcctttt tctctgacgt gggccagtct aaaaagcgtc
     2581 caccgtcgag ctcttccttt ccgtttgata cccgggcaac aatttgttga ataagggcgt
     2641 tcaagtatgt ttgctgttct ttcaggtatg ctaaatctcc ctgatgcagg tataagtccc
     2701 ggtgaataat aatccaccat aaagaatagg aggagatggt gttcatccat ccgggtaaag
     2761 gagttgtgtc tcgggcaaaa tcgaggcttt tcctgaccac ttcatttttc ccgaataccg
     2821 tgtttattgt catcacctcc ggatgcatgt cgccgaccca aacaagccgg tcgcgtttaa
     2881 tcccgtccca gaggtactcc tgcatgttca aatggacagt atatgcaccg gtttcccata
     2941 ttctgtttag cctttcgttg tcgcacctga aggatccggt ataaggaatg tcccggtacc
     3001 ggaagatggc cctgaccgcc tttaacggaa gatcttcgtt caaatccaac agatcaattc
     3061 gtacgaacct gaatcctgaa tttcccactt caacacttcc cagccatggc aactcgatga
     3121 taaaatccct taatgaatga tcgttagtgg cagaattcat tccgggaaca gagttgtcaa
     3181 tgctgttact catcgcttcc gagaccgatt ctcccagcct tacccttacc tttagcggtt
     3241 ttttgtttcc cctgattccg gctgctagct ggagaccgcc atagagttcc gtcccaaaat
     3301 ccagtaaaac cgatgcctgt ttatctgtat cggaacgcat gacgcacatt ccggcaccgc
     3361 ttgtggatag ctgaccgtca aattctttca aaagaacctc cgggtttttt acatgctctc
     3421 ccgtttgatc ggaagaccag actagtttgg cgggagtgac gtaactttga gtcatatcat
     3481 ccttttgtaa tctgttctca aaagcgtttt tcaaaatatt gttttgagaa aacagagtgc
     3541 ttgctccaag caaaattatt gaaaacagta aactcttgat tttcattttt aaataattaa
     3601 atgtttattc cgtttttctc ggatccacta actgcggcat ccagacttta aaaaaggggt
     3661 aggtgcccat tgtattgcca gcagaagcat agtcgcataa atttacttca acgggttctg
     3721 cgttgtattc ggtataagct tcgggcagga acttagcgga aaaaaccatc caagcatcgg
     3781 aattatggtt tttttgggga gttaagtgga taaatccgtt cttgtctttt attggagcga
     3841 ttacggcttc gagtttaggg ccggccaacc tttcgtctcg cgttaagaca acaggtccgc
     3901 gtgtgatagc aacgtttacc gggctttgac ccgtataatg gagttcccct ttcatatcca
     3961 atctgatttg aatcttatca ccctctttcc aggtacggct gattttcagg taatcgcccc
     4021 gagtcacctt ttcaaccggc tggtcgttga ttaaaacggt tgactcatca ctccaagagg
     4081 gaatccttaa ggccaacgtc atgttttcag ccgttttcat ttcaagcgat atattcacat
     4141 ttcccgaaac aggataatcc gtatgttgtt ttacggtaat tacatttttt tggggtgaag
     4201 ttgtttgata agaaccttcc atgtagaaat ttatattcag gccttcgttg ctcgacataa
     4261 ctgctgttaa gggaatagtg aataatccgc gaggcccgct ggcattgcag cagttcaatc
     4321 ccattccaca ttgctccgat ccgggtagcc tttggccgga aagcggtgta tattttgccc
     4381 agtcagaccc atcgggtctc attgctccca acaaagcatt gtaatatgcc cattcaatgg
     4441 cgtcggcata ctttgactcg ccggtcagcc tgagtaattg ttgattgaat tttatccacg
     4501 taacggtcac gcacgtctcc tgataatgcg ctatggggac gtgctggatt ttttttccgg
     4561 agaaccaggc ttccattgcc gcacccgaac ctgcaatatt tatttcggta tccgtaatgc
     4621 tttgccaggt ttgctctgca gcttttttgt aagattcttt tcctgtaatc cggtagagct
     4681 caagcaaccc ttcgtagcac gacatcattt catatgcttt ttgtccctgt tcccatccgt
     4741 accaattttc agccggtttt gcaaatcgtt gacccacagg aaccgatgct ttggaaataa
     4801 gctgaggccc ttctggcgtt tcccattgac gaacaatttc ttccgcaaaa tccagatacc
     4861 tttcattccc ggttcgcatg tagagctgac aaaccggttc caggacagaa gaggcagcca
     4921 tacctctgtg gttacccatt tttacaatca gggcattttt atccgctaat tcctggataa
     4981 gaaaatctgc ttcttttgca gccgcttcca atgcctttct gtcttttgat acatcgtaat
     5041 aagcaattaa acccagcata acgtattttc ttccccagat atcccacgct ttcaaccggc
     5101 tttcggaggc ataattgcca atgtagcctt cgggagtctg tgttgccatc agttcgtacg
     5161 ctgcattttt aagcttattg ataagttgct ggtcagggcg gtaggaataa gccagtatgg
     5221 ctgaagtgaa ccatttgccc cagaattcgc tctgccagca gctgtgctca tcgtggattg
     5281 tgaacggacg gatcaaccgg tgagcatcct gtgccaatac ccggttgttg aaagaagcat
     5341 caagcttttc tccaatatgg ccttcaatat ttgccgttgt tgcaggtgaa agtttgtcct
     5401 gaacagctga ttttactttt ttttggggaa ataaatgaaa gctgctgaaa aacaagatca
     5461 gcaacaggta atacttcatg agaataaatt tttattgtaa atctcagtta tctatttttc
     5521 tgttttcatt ccatgaatcc agcgtttagg tccctatacc agactttccc tgggcaaagc
     5581 taagaaaaat tgcgaaggaa agccggtaaa aggcactaaa aacagtcgtt tcatgagttg
     5641 tttactgcaa tctctttttg aaactgtatt ttccgctttt cacggcggtg taattggttt
     5701 cctgaggcag atatactttt gcttcgcaat taatgggaac ctcgatatcc atttcgaact
     5761 ggccgttatc caccttccag tccaccttga tggtcccgta taaggtgttg taagaggcgt
     5821 ttacatacgt taagtcacct acgacctgcg gcgcgatttt gaagttcttg aatccggtgt
     5881 atgcgttttc ttctacatca atacctgcca acgacctata gaaccactct tctatctgtc
     5941 ccatcataaa atgattccac gaagcgcctt ttctcggatc ccaaagctcc gtaagtgtgg
     6001 tggctccaaa ctgaagctgg aagccgtaac ctggaacttc gttgtggtta tgcatcttgt
     6061 acatcacttc attcaatccg tttcttgcca gtgcctgaaa caagtatcgg ttccccacat
     6121 caccggtgga gagtctgtat cctttttctt cgatatcctt gaccaggttg tcgaggaccg
     6181 attgtttgtc ttgggggtta accaatcctg caaacaaagg catggcgttg gatgcctggc
     6241 ttccggttcc gtactgtttg gtgtcttcgt tgaagaactc cttgttaaaa gcggttctta
     6301 cctcctcccg gagattggaa tagaatgtta tgtcttcttt tttactaagc atctcggccg
     6361 cttgcaccaa ataatcaatc accatgaagt aatgagttgt tgcggataat gagatggggg
     6421 tattgtgtga tacgccataa ggctgatctt cccggtaatc gcaccaatcg cccaacccgt
     6481 gcgaaacgat gtggtctttt gctgttgtgg ttaggtagtc gacatacctt ttcatgacat
     6541 cgtaatgttc agctataagc gagttgtctc cgtaaaactc gtagtacata aacgggaaaa
     6601 taacgctggc acttccccat tcgggggagt cccgaaatcc accgttgaaa actacgtatt
     6661 cgggagcaat acttgttacc aaaccgttcg gtaactgtgc atcgcgaatg tcctgcatga
     6721 ttttaggagc aaacctggcc aagttgaaat tatagtataa cccgggtcca ttcaggtgca
     6781 cttcttccag ccatccgagt ttttccctgt gcggacaatc ggtaaatacg gcttgcatat
     6841 tgcttttaat ggcattcaca atcaggttgt gcgcgttatt gaagatctcg ttggaagaat
     6901 gaaaactacc tgccggttcg gctgagttat aaacaaaaca ggattttatc gcttttatga
     6961 ccggaatatc cctgttgtct tctgattccg ataacttggc tccatcaact tggatatacc
     7021 ggaacccgta ataggaaaaa cggggttgcc aggtctcttc gctttctcct tttagtgtat
     7081 attcgtagta atagggcccg ccggactgtg tttgatttat actgccgtcg tcgtttatcc
     7141 gttcggcaac ggtcagacga attttgtcac cccgttttcc ctttacggta aaggccggat
     7201 atccggagag gttttgtccc atatccaaca catacgattc acctgttttc atactctctt
     7261 taatggagaa atattccatt tcccttacag gggttgaagt ttgcggtttt aactccccat
     7321 taggagcatt atccacaaca acaggaagcc attgtgaatc atcaaagcca ggcgtgtccc
     7381 atcctggttg ttcaagacgc gcatcgtaat cttctccacc atacatactg ttaaagacaa
     7441 tggggctggg agaatatttc cagttttcat cggatatgat ctcttctttt gtgccgtctg
     7501 tgtaggtaat ggatattttt aggaacaggg taggagggcc gaagcttacc tgcattttct
     7561 tgtatcgtcc gccttgctcg ttgtagaatc cgtttccgag gagcacacct acggtattat
     7621 ctcccttttt tactccctct gtgaggtcgt atgcgttgta atagacggtt ttgtcataat
     7681 cactccacag cggattgtac tggtcgttac cgattttctt tccgtttaat gagagttcgt
     7741 agtgccccaa tccgctgatg tagataattg catcctcgat ccgtttttga gc
//
LOCUS       DAOSDR010000109         7765 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrimonas sp. isolate AMR_MDS_1826
            NODE_3463_length_7765_cov_3.922827, whole genome shotgun sequence.
ACCESSION   DAOSDR010000109 DAOSDR010000000
VERSION     DAOSDR010000109.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863355
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrimonas sp. (wastewater metagenome)
  ORGANISM  Petrimonas sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Dysgonomonadaceae; Petrimonas.
REFERENCE   1  (bases 1 to 7765)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7765)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:10:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,720
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 35
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7765
                     /organism="Petrimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3463_length_7765_cov_3.922827"
                     /isolate="AMR_MDS_1826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2023866"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1146)
                     /locus_tag="PLI30_05705"
     CDS             complement(<1..1146)
                     /locus_tag="PLI30_05705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="lysyl endopeptidase"
                     /protein_id="HOI79085.1"
                     /translation="MPIPKNIQTILIPFLLSLLTSSGFAQISHGGRPLPVNASAYTRN
                     LQRLETPFVEMPSFDVQQAMLRSEAEGKKFKSLEFAHKFHVFLRPDNSGVTFNTLDNR
                     KIWRVGIRSRKAYSLNILFSKFRLPEGANVFVYNADQSEVLGSYTQENNTELNLLPVQ
                     PIAGDEIIVEYQEPLDAAFKGEIEIGEVNHDFRGLFRATEPRDPRQSCHPNLVCYPED
                     VQPGSGVVALIINGTTYCTGSLVNNSAEDGSPYLLTATHCINRDYDPAFLANRRYDMV
                     AGSIVAFFAYQSPVCDKEIRGPLQMTMASADSVLISERYDISLLKFKQTPPKEYQPYY
                     LGWNVSASPQPPFHGIHHPNGGTKKVAIENDPLAIGSFDDPRYNMEPN"
     gene            1233..2015
                     /locus_tag="PLI30_05710"
     CDS             1233..2015
                     /locus_tag="PLI30_05710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006797643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02757 family protein"
                     /protein_id="HOI79086.1"
                     /translation="MLILQKNLKDFLDRKADFYNRTSFIENDPIFVPHQFTLKQDIEI
                     MGFFAATFAWGQRKTIIQKSMELARRFDGKPHEFILHHSESDLKQLLGFRHRTFNDTD
                     LLWFVDFLHRHYKESESLEEAFLPETGFTDMEGALIHFGQYFFNAPDAPGRTRKHVAS
                     PERNSTCKRLNMFLRWMVRCDGKGVDFGLWKRIQPAVLICPVDLHVDRTARRLGLVTR
                     RQTDWRTAVELTENLRLLDACDPVKYDFALFGLSIEKEIYDL"
     gene            complement(2026..3139)
                     /gene="prfB"
                     /locus_tag="PLI30_05715"
     CDS             complement(join(2026..3075,3077..3139))
                     /gene="prfB"
                     /locus_tag="PLI30_05715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006256020.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HOI79087.1"
                     /translation="MITADQLKNVLEREQALRGYLDIDAKTIQLEEEELKTQAPDFWN
                     DNKAAEAQMKIVRELKGWLNAYDKVKSATEELQLAFDFVKEGIVSEEEVDTNYGRALE
                     LIENLELKNMLRREEDQLGAVLKINAGAGGTESQDWASMLFRMYQRWCESKGYKTTVT
                     NWQDGDDAGIKTATLQVEGDIAYGFLKSENGVHRLVRVSPYNAQGKRMTSFASVFVVP
                     LVDDSIEIYVNPANISWDTFRSSGAGGQNVNKVETGVRLHYLYKDPDTGEEQEIVIEN
                     TESRSQMGNRENAMRLLKSQLYEIELEKRRTAQAQIEAGKKKIEWGSQIRSYVFDDRR
                     VKDHRTNYQTSDVTGVMDGDLDDFIKAYLMEFGGEA"
     gene            complement(3256..4230)
                     /locus_tag="PLI30_05720"
     CDS             complement(3256..4230)
                     /locus_tag="PLI30_05720"
                     /inference="COORDINATES: protein motif:HMM:NF013073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HOI79088.1"
                     /translation="MHKKTVLLTLLAVLMVSCGVKKTTVTPSKKLTGTGQEVINYGMR
                     YLNKPYRFAGKGPHAFDCSGFTSFVFREFGYRLSTSSAGQDRQMPSIRQREHLQVGDL
                     VFFEGRARNGRVGHVGIVKELLPGGEFRFLHASTTSGVIVSLSTEPYYASRYLRGGRI
                     LQENNALAIAEKVPRDKPKQVKKGDTRRNAFTSAKAKEQVLIEAPKTKSEKETADIPQ
                     NPETKELIVHSRPVNQLLTDLPKNKQGQEEVIKPAVQTMVRPDTISVPEPAETKIIEN
                     DSITHPVKPGETLYSISRKYNCSVAQLRDWNPQLGTVLKPGEKLVIRP"
     gene            4412..5020
                     /locus_tag="PLI30_05725"
     CDS             4412..5020
                     /locus_tag="PLI30_05725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019538692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HOI79089.1"
                     /translation="MEQIKNIVFDFGGVLIDWNPVYLYSKIFEDRAEMEYFLNNVCTY
                     PWNVLQDAGRPVALATAEKQQEFPQYKDEIAMYYGRWAEMLGGEISENSRLVKLLSKN
                     YNTYGLTNWSAETIPVAMERYEFFNYLKGMVVSGDEKIVKPDPKLYYILLDRFSIDPE
                     ETLFIDDNAHNIDTARLSGFRTIHLVPEMNLKEELVKMGVLL"
     gene            5317..6621
                     /gene="metK"
                     /locus_tag="PLI30_05730"
     CDS             5317..6621
                     /gene="metK"
                     /locus_tag="PLI30_05730"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005853916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HOI79090.1"
                     /translation="MNYFFTSESVSEGHPDKVADQISDALLDEFLAYDPNSKVACETL
                     VTTGQVVLAGEVKSNTYVDVAEVARKVIAGIGYTKSEYQFEAKSCGVFSSIHEQSDDI
                     NRGVDGKADPMDQGAGDQGMMFGYATAETDNYMPLALDLSHALLRELANIRKNELELM
                     PYLRPDAKSQVTIEYSPDGTPVRIDTIVISTQHDEFIYPESNTREAVLKADAAMQQQI
                     ANDVKTILIPRVREKYKHTRDIHKLFDDKIKYHINPTGKFVIGGPHGDTGLTGRKIIV
                     DTYGGKASHGGGAFSGKDPSKVDRSAAYAARHIAKNLVAAGVASEVLIQVSYAIGVAH
                     PMNIYVNTFGKSRVDMADAEIAEKVKTLFDMRPKAIENRLKLRNPIYLETASYGHMGR
                     QPEIVTKKFESRYMPETVVKEVELFTWEKLDYVDVIQKEFGL"
     gene            6628..7239
                     /locus_tag="PLI30_05735"
     CDS             6628..7239
                     /locus_tag="PLI30_05735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005676832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HOI79091.1"
                     /translation="MKRNLSEKNIREVVVYCASSPHIGEAYFDIARQLGKVLANNDIT
                     CITGGGKQGLMGAVNDSVLENGGKVKGIIPQFMIDSGWCHPQLSELVVTESMHERKFL
                     MAKQSDAAVALPGGFGTLEELAEILTWKQLGLYKNAVVILNINGYYDPLLAMFDKMIC
                     EKFLHHNYRNLWQVVDSPEKAIDYLQNGETWNPSFTKYDKKEL"
     gene            7262..>7765
                     /locus_tag="PLI30_05740"
     CDS             7262..>7765
                     /locus_tag="PLI30_05740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI79092.1"
                     /translation="MPERIPQIPVDDVIGQSSDSIRVGFLSFEYIPTDNTGLFFNVDS
                     STFSVSSGQMPEGFSGALLPFSQTVHSVFFLFFTFCFVIVAFWFNREGLTLVANFRNI
                     FSGGMRRRTIFKEEITTTGIWSEFFLIFQTILILTVVFFTFFWDKGISGLSVKNMVLV
                     FLAVFLGF"
BASE COUNT         1939 a         1673 c         1873 g         2280 t
ORIGIN      
        1 gttgggttcc atgttgtatc tcgggtcatc gaaagagcca attgccaatg gatcattttc
       61 aatggctacc ttctttgtac ctccattcgg atgatggatt ccgtgaaaag gaggttgggg
      121 agaagcgctg acgttccatc ctaaataata aggctggtat tctttcgggg gagtttgctt
      181 gaatttcaac agggagatgt cgtatcgttc actgattagg accgaatcgg cagaagccat
      241 ggtcatttgt aacggcccac gtatttcttt atcacaaacc ggagattgat aggcgaaaaa
      301 ggccacgatg cttcctgcga ccatatcgta cctgcggttt gccaaaaagg cggggtcgta
      361 gtctctgttg atacaatgtg tagccgttag caggtatgga gatccgtctt cagccgaatt
      421 attcacgagc gatccggtgc aataagtggt tccattgata ataagagcca ctacgccact
      481 gccgggctga acatcttcgg ggtaacagac caggttggga tggcaggact gccggggatc
      541 tctgggttcc gttgctctaa acagtccgcg gaaatcgtga ttcacttctc cgatctctat
      601 ctctccttta aaagcggcat ccaacggctc ctggtactcc acgatgatct catcaccggc
      661 gatgggctgg acgggaagga ggtttaattc ggtattgttt tcctgggtgt atgatcccaa
      721 gacctcagat tggtcggcgt tgtacacaaa cacgttcgct ccttcgggta aacggaattt
      781 cgaaaaaaga atattcaggg agtatgcttt tctcgaccgg atccccaccc gccatatttt
      841 tctgttgtct aacgtattga aagtgacacc tgagttatcg ggacgaagaa atacgtgaaa
      901 tttgtgggca aactccaggc ttttaaactt tttgccttcg gcttctgagc gtagcatcgc
      961 ttgctgaaca tcaaacgatg gcatttcaac aaagggagtc tccagtcttt gcaggtttcg
     1021 ggtatatgca ctggcattta caggcagcgg tcgcccgccg tggcttatct gggcaaaacc
     1081 ggaagaagta aggagtgata acaggaatgg aatgaggatt gtttgaatat ttttaggtat
     1141 gggcatactg ttcgtaatta agattgaccg ttgcaaattt aagacgtttt taataaataa
     1201 gattactttt gtggtgtttt attccgatgc acatgcttat tttacagaag aacctgaaag
     1261 atttcctcga ccggaaggcc gacttttaca acaggacatc atttattgaa aacgatccaa
     1321 tctttgtgcc gcaccagttc accttgaagc aggacattga aattatgggc ttttttgccg
     1381 ctacgttcgc ttggggacaa cggaaaacga ttattcagaa atccatggag ctggcacggc
     1441 gattcgacgg caagccccac gaatttattt tgcaccattc cgaatccgat ttgaaacagc
     1501 tgctgggatt caggcaccgt acatttaacg ataccgattt actctggttc gtggattttc
     1561 tacaccggca ttataaagag agtgaaagcc tggaagaagc ctttctccct gaaaccgggt
     1621 ttacggacat ggagggtgcg ttgattcatt tcgggcaata cttcttcaat gctcccgatg
     1681 cacccgggcg cacccggaaa cacgtggcat ccccggaaag aaattcaacc tgcaagcggc
     1741 tcaatatgtt ccttcgctgg atggtgcgtt gcgatggaaa aggtgtggat ttcggtttat
     1801 ggaaaaggat tcaaccggca gtgctgattt gtcccgttga cctgcatgtg gacagaacag
     1861 ctcgtcgcct gggtttagtc acacggcggc aaacggactg gagaacagct gtggaactca
     1921 ccgaaaacct ccgactattg gatgcatgtg atcctgtaaa atacgatttc gccctttttg
     1981 ggctctctat tgagaaggaa atatatgatc tgtagtccaa tcgttttacg cttcccctcc
     2041 aaactccata aggtatgctt tgatgaaatc atccaggtcg ccgtccatta cgccggtgac
     2101 gtctgaagtc tggtaattgg tgcggtgatc ttttacccgc cgatcgtcga agacataact
     2161 gcggatttgt gaaccccatt ctattttctt ttttcctgct tcgatttgtg cttgtgccgt
     2221 ccggcgtttt tcgagttcga tttcatagag ttgtgacttt agcaaccgca tggcgttctc
     2281 acggtttccc atttgtgagc gactctcggt attctcgata acaatttcct gctcctcgcc
     2341 tgtgtccgga tccttgtaga ggtaatgtag ccgtacaccg gtttctacct tgtttacgtt
     2401 ctgtccgcct gctcccgatg atcggaaggt atcccaggaa atattggccg ggtttacgta
     2461 aatttcgatg ctatcatcca ccaatggtac cacaaagacc gatgcaaacg acgtcatgcg
     2521 cttgccctgc gcgttgtatg gggaaaccct gaccagccga tgtacaccgt tctcgctttt
     2581 cagaaagcca taggcaatgt cgccttccac ttgtagggtg gctgttttta taccggcgtc
     2641 gtcgccgtcc tgccagttgg ttacagtagt tttgtaccct ttgctttcgc accagcgctg
     2701 atacatgcgg aacaacatcg acgcccagtc ctggctttcg gtcccgccgg ctccggcgtt
     2761 tattttcaat accgcaccca attgatcctc ttcgcgacgg agcatgtttt tcagctccag
     2821 gttttcgatc agttcgagtg cccggccgta attggtgtct acctcttctt cgctgactat
     2881 gccttcttta acaaaatcga atgccaattg taactcctcg gtggccgatt ttaccttgtc
     2941 gtaggcattg agccagcctt tcagttcacg cacgattttc atctgggctt cggcagcttt
     3001 attgtcgttc cagaaatcgg gagcttgtgt tttcagttct tcttcttcga gttggatagt
     3061 ctttgcatcg atgtcaaaga taccccctca gcgcttgctc gcgctccaac acatttttaa
     3121 gttggtctgc agttatcata ttttttgaga ttgaatgatt gaattggttt ttaccttttt
     3181 tcagggtgca aaaatacgca aaattcgcca tcagccaaaa catttgtcct gctgaaatct
     3241 ggagtcaccc gttgttcagg gcctgatgac aagtttttca cccggcttta aaaccgttcc
     3301 aagctgtgga ttccagtccc ttagttgcgc tacggagcag ttgtatttcc gggagatgga
     3361 gtaaagtgtt tcacccggtt ttaccgggtg tgtaatagag tcattttcaa tgatttttgt
     3421 ttcagcgggt tccgggacgg aaattgtatc gggacgcacc attgtctgaa ctgccggctt
     3481 tatgacttct tcctgtccct gtttgttttt aggtaagtcg gtgagcaatt gatttacagg
     3541 gcgggaatga acaataagtt cctttgtttc gggattttgc gggatgtcag ctgtctcttt
     3601 ttcgcttttt gtcttaggtg cttcgattaa aacctgttcc ttggctttag ccgaggtgaa
     3661 agcatttctc cgggtgtcgc ctttttttac ctgtttgggc ttgtctcgcg gaactttttc
     3721 agctatagcc aacgcattgt tttcttgcag gattcttccg ccacgcaggt aacgggatgc
     3781 gtaataaggt tcggtggaaa gagagacaat aactccactt gtggttgatg cgtggagaaa
     3841 gcgaaactct ccgcccggaa gcaattcttt cacgatacct acgtgtccga ctctaccgtt
     3901 gcgtgcacgt ccctcgaaga agactagatc acccacctgt agatgctctc gctgcctgat
     3961 gctgggcatc tgtcggtctt gtcctgccga actcgtgctc agcctgtacc caaactccct
     4021 gaaaacaaat gaggtgaagc ccgaacaatc aaatgcatgt ggcccttttc cggcaaaccg
     4081 gtacggtttg ttcaggtacc gcattccata attgatgacc tcctgtccgg tgcccgtgag
     4141 ttttttactg ggcgttacgg ttgttttttt tactccgcag gaaaccatta ggacggcaag
     4201 aagtgtcagc agtacagttt ttttatgcat tcattccgtc tttttgtgtg agctcatttt
     4261 tttttgttgt gcaaaaatag gaaaatatcc ttgatgaagc atacgggaat ccttccttta
     4321 actaattatc cgaaatttta ggacttgaag aaagcgttgg atgtaaagaa aaagcactat
     4381 ttttgcattt tatcgcaatt aaggcaaaac aatggagcaa attaaaaaca tcgttttcga
     4441 ttttggtgga gtgttaatcg actggaatcc tgtatatctt tacagtaaga tttttgaaga
     4501 tcgtgccgag atggagtatt ttctgaacaa tgtttgcacg tatccgtgga acgtccttca
     4561 ggatgccggc cgtcctgtcg cactcgcaac agctgaaaag cagcaggagt ttcctcaata
     4621 caaggatgaa attgcgatgt attatggccg gtgggcagaa atgctgggtg gtgagattag
     4681 tgaaaactcc cggttggtga agctcctgag caaaaattac aacacctacg gactgaccaa
     4741 ctggtcggcc gagaccattc ccgtggccat ggagcggtat gaattcttca actatttaaa
     4801 aggaatggtg gtttcgggtg acgagaaaat cgtaaaaccc gacccgaaat tgtattatat
     4861 tttattggat cgattcagta tagaccctga agagacgttg tttatcgatg ataatgcaca
     4921 caacatagat actgccagac tgtcgggctt taggactata catcttgttc cggaaatgaa
     4981 cctgaaggaa gagttggtga agatgggggt gttgttataa ataatacttt ttaacctttg
     5041 gcaacacgct atttgccaat tatttcctat ctttgcatcc gaaaataatc gtgtggacag
     5101 atttgaactg cttgcaacca cagaaaaaaa tgctaaaacg cccagctgtt cgctattttt
     5161 tcttgcatgc acttcctttt ctctcccgac tggttacgta aattttatta atcacaaagc
     5221 cgataatcaa aggtttggga taacagtact tttatggttt aaaaatcaaa tagcctagca
     5281 tcctcaatgt tgattttggt ccttttatct taaaaaatga attatttctt tacctccgaa
     5341 tcggtgtccg agggacaccc cgataaagta gccgatcaga tatcggatgc tttgctggac
     5401 gagtttctgg catatgaccc taactcgaaa gtagcttgcg aaacgttggt gacaaccggt
     5461 caggttgttc ttgccggtga agttaaatca aatacgtatg tggatgttgc tgaggtggcg
     5521 cgtaaggtta ttgccggtat cggctacacc aaaagcgaat accagtttga agctaaatcg
     5581 tgtggtgttt tttcgagcat ccacgaacaa tccgacgaca taaaccgggg ggttgacggc
     5641 aaagcagacc cgatggacca gggcgccggt gatcagggga tgatgtttgg ttatgcaaca
     5701 gcggaaaccg ataactatat gccgttggca ttggatttga gccacgcatt gttgcgcgaa
     5761 ttggctaaca tccggaaaaa cgaactggaa ctgatgccct acctccgtcc cgatgccaaa
     5821 tcgcaggtta ccattgagta ttccccggac ggaactcctg tccgcattga tacaatcgtg
     5881 atttccactc agcacgatga gtttatttat cccgaaagta atacaaggga ggcggtttta
     5941 aaggcggatg cggccatgca gcaacaaata gccaatgatg tgaaaacgat tctgattccg
     6001 cgtgtccgtg agaagtataa acatacccgg gacattcata aacttttcga cgataagata
     6061 aagtaccaca taaatcctac cggaaaattc gttatcggtg gcccgcacgg agatacggga
     6121 ttgaccggac gcaaaatcat cgtggatact tacggaggga aagcgtcgca cggcggcggt
     6181 gcattttccg ggaaggatcc gtctaaagtg gatcgttcag cagcttatgc ggcacgccat
     6241 atcgccaaaa acctggttgc tgcgggagtg gcatctgaag tattgataca ggtttcgtac
     6301 gccatcggtg tggcacatcc tatgaacatt tatgtgaata ccttcgggaa gagtagggta
     6361 gatatggctg atgctgaaat cgccgaaaaa gtaaaaaccc tgttcgatat gcgtcctaag
     6421 gctattgaga accggttgaa acttcgtaat ccgatatatt tggaaacggc ttcctacgga
     6481 catatgggcc gtcaaccgga aattgttacg aagaaatttg aatctcgtta tatgccggag
     6541 acagtcgtca aggaagtcga gctttttacc tgggaaaaac tggattatgt ggatgtaatt
     6601 cagaaagaat ttgggttata ataaaaaatg aaacggaatt tatctgaaaa aaatatcaga
     6661 gaggtggttg tttactgcgc ttcgagccct cacattggag aagcgtattt cgatattgcc
     6721 cggcaattgg gaaaagtctt ggcaaataac gacatcacgt gtataaccgg tggtggaaaa
     6781 cagggattga tgggggcagt aaacgatagt gtgctggaga acgggggaaa agtaaaaggg
     6841 attattccac aatttatgat cgattcgggg tggtgccatc ctcagttatc ggagctggtt
     6901 gttaccgaat cgatgcacga gcgtaaattt ttgatggcta agcaatccga tgctgccgtt
     6961 gcgctgccag gcggattcgg gacattggaa gagttagcgg agattctcac gtggaagcag
     7021 ttgggactct acaaaaatgc agttgttatt ctcaatataa acggttatta cgatccttta
     7081 ctggcgatgt ttgataaaat gatctgcgaa aaatttcttc atcacaatta tcgtaattta
     7141 tggcaggttg ttgactctcc ggaaaaagcg attgattacc tgcaaaatgg tgagacatgg
     7201 aacccctctt ttaccaaata tgacaaaaaa gaattgtaat tttgttgaaa atttgtaagg
     7261 tgtgcccgaa agaattcccc aaataccggt agatgacgtt attggacaat catccgattc
     7321 aattcgggtc ggatttttgt cgtttgagta tattcctaca gataacaccg ggcttttttt
     7381 taatgtcgac agttctactt tctcggtttc atcggggcaa atgccggagg gattttccgg
     7441 agcgcttctt cccttttctc aaaccgttca tagcgttttt tttctcttct ttaccttctg
     7501 ttttgttatt gttgctttct ggtttaacag agaaggcctt acgttggtgg ctaacttcag
     7561 aaatatcttt tcgggaggaa tgcgaagacg gacaatcttt aaggaagaga ttactacaac
     7621 cggaatctgg agcgagtttt ttttgatttt tcaaaccatc ctgattttaa ctgtcgtttt
     7681 ttttactttt ttttgggata aaggtatttc cggcttatcc gttaaaaaca tggtgttggt
     7741 ctttctggct gtttttctcg gtttt
//
LOCUS       DAOSDR010000110         7741 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrimonas sp. isolate AMR_MDS_1826
            NODE_3477_length_7741_cov_4.032917, whole genome shotgun sequence.
ACCESSION   DAOSDR010000110 DAOSDR010000000
VERSION     DAOSDR010000110.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863355
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrimonas sp. (wastewater metagenome)
  ORGANISM  Petrimonas sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Dysgonomonadaceae; Petrimonas.
REFERENCE   1  (bases 1 to 7741)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7741)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:10:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,720
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 35
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7741
                     /organism="Petrimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3477_length_7741_cov_4.032917"
                     /isolate="AMR_MDS_1826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2023866"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..483
                     /locus_tag="PLI30_05745"
     CDS             <1..483
                     /locus_tag="PLI30_05745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011967062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction DNA helicase RuvB C-terminal
                     domain-containing protein"
                     /protein_id="HOI79093.1"
                     /translation="LEYYDADTLTGIILRSANILNIACARNAAEEIASRSRGTPRIAN
                     ALLRRVRDFAQVKGSGKIDKAISSYALEALNIDRYGLDEIDNKILLTIIDKFKGGPVG
                     ITTIATAVGDDAGTIEEVYEPFLIKEGFLKRTPRGREATELAYRHLGRKRDLEQGLLF
                     "
     gene            complement(613..1995)
                     /locus_tag="PLI30_05750"
     CDS             complement(613..1995)
                     /locus_tag="PLI30_05750"
                     /inference="COORDINATES: protein motif:HMM:NF025458.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4270 domain-containing protein"
                     /protein_id="HOI79094.1"
                     /translation="MKLQTLYKLLFIAFVTVLASCVDTLDKIGFTIQPENDRVSVGTD
                     TLYVSSRTIQVDSVFSRTKYPILGEYIDPVFGSIRSEYVGEFYYPENQSFKDGAIIDS
                     VRLTVSYSSIIGDSLAPMRLAVYKVIEELPKNKDYTNFDPRTKSDMSNPLGTKTFTGK
                     NNTYRTETYYTGNTTQTVKIYEIYTELPKSIGQSFLDEFKKPDHGELKNTDTFRKFFP
                     GLYITTNFGNSTILNVNLTSLNIFYKYLDPKGSSQKTDTIRTSEFRLNITPEVTQINH
                     IQNNNDQLLAPNDKGTFVKSPAGVNTEVTFPISEIYSKLTNRSLNQARLMVYALPEAN
                     QDEKVKLSPPDHLLLVNKDSLKGFFENRRLHDNVTSFYASFDKETYSYNFGNIAAMIN
                     YYKRQKKEAFDLTYYLVPVDITFTTTGSSYYSSGTSTPTAIYNQMKPSAVMLENKPEK
                     LKLDIIYSSF"
     gene            complement(2137..2955)
                     /locus_tag="PLI30_05755"
     CDS             complement(2137..2955)
                     /locus_tag="PLI30_05755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019537967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch synthase"
                     /protein_id="HOI79095.1"
                     /translation="MSQKRILYITQEIFPYLPETQISHISRYLPQAIQDKGHEIRTFM
                     PKFGNINERRNQLHEVIRLSGMNLIIDDSDHSLIIKVASIQSARMQVYFIDNDDFFQN
                     RETLTDQKGEEYDDNDERSIFFVRGVLETIKKLRWVPDIIHCHGWFTALAPIYIKKGY
                     KDDPCLKNAKVIYSVYDENFEKPFNESFSEKLRFDTIGDKEIEDVKKRGKMDFVNLTK
                     LAIDYSDGVVQGSENIHKELDKYIIDKKIPYLGYTPDFDNNVEKIGEFCDKIDA"
     gene            3169..4491
                     /locus_tag="PLI30_05760"
     CDS             3169..4491
                     /locus_tag="PLI30_05760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005681581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HOI79096.1"
                     /translation="MYSNKHVVKISLPILLSLLAQNIIQVIDTAFLGRVGEVELGASA
                     LAGIIYIAIYTIGFGFSMGSQILIGRRNGEKKFHQIGEIVIQGILFLLVPALLLIAVF
                     KLGFTDTLMRLFESENVSEAVSDYLDWRVYGFVFAFANSTFRAFYIGIARTKVLTINS
                     LVMALVNLVLDYGLIFGNFGLPEMGIGGAALASVIAEASSTLFFIVYTRKTVDLEKYG
                     FRRITFKWSVVKKVLDISIYMMLQYLLSIVTWLMFFVFIENYLGERSLAVTNIVRSLY
                     TIVTIPSHALGSAVNTMVSNTIGAGRQNEVLNLIKRVTLISLFSMLAIILIVAVAPRL
                     MIHIYTNDTSLIDDTVAPLYVLLTSLPFYAIGTVLFSAVSGTGNTQRALFYEIITLSG
                     YVLYTWFIVVYLRLSVGWAWTTEHVYWGQLMFFSLFYLRSKKWVHKKI"
     gene            4530..5597
                     /locus_tag="PLI30_05765"
     CDS             4530..5597
                     /locus_tag="PLI30_05765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI79097.1"
                     /translation="MKHRFLFFLVVFFCSSVYAQEYRWKVGLDYFFDNTEYGKSSFTD
                     SETMNGIWLNPMGSIGWGEKHSIRAGVNLLKIPGMDKIVDKVEVSLCYQYKSPAMVFR
                     AGSFPRSEVLGNYNNFFFKDSVNHFVPLMQGVFWQIGSNRNFLNMWFDRTGYATRTRH
                     EHFFVGLSGKISNGLLFADFQSYMFHYSNTKPATPGEGVSENLQLQATTGLDYEGEGG
                     FKGLIAVGTLMGYERDRRFESERYKPVGFVARGDAEMWGIGTKNTFYRGEPRMRLAGT
                     FGGNLYWGTQFLRAKSYAESEWYIRLIESDFVKARFNCNLHFTEGNVLFQQMLTVSAS
                     IGNFTPKNRKTTRYPWMRIFH"
     gene            5627..7696
                     /gene="ligA"
                     /locus_tag="PLI30_05770"
     CDS             5627..7696
                     /gene="ligA"
                     /locus_tag="PLI30_05770"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019537962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HOI79098.1"
                     /translation="MKDLFSNNNIQPAESPSVGGNSAAKDKLETLRKQLHEHNYNYYV
                     LSQPTISDIDFDALMQQLAELEKEYPEFYDPNSPTMRVGSDINKNFVQVQHRYPMLSL
                     QNTYTEEEIADFFNRVKRSLNEDFEIVCELKFDGTSISLVYENGRLSQAITRGDGKQG
                     DDVTANVRTVRNIPLVLKGDNIPGYVEVRGEILMPWSVFEELNREREVQEEPLFANPR
                     NAASGTLKMQDSKVVAARKLESSVYYLMGEGLPSDSHFENMELARKWGLNVSATMKKC
                     CSLEEVFEFLKYWDVARKSLSVATDGVVLKVDSLSQQRNLGSTSKFPRWAIAYKFNAE
                     KALTRLESVTYQVGRTGAVTPVANLEPVLLSGTTVKRASLYNEDAILALDLHIGDRVY
                     VEKGGEIIPKITGVDKEARFLIGDKVRFVTRCPDCDTPLIRNEDEAVHYCPNSENCPP
                     QIKGRIEHFVTRRAMNITMGPETIGLLYDKGLIRDAADLYALQFEDLVSLERWAETSA
                     NNLLASIEKSKTVPYERVLFALGIRFVGETVAQKLALAFHDIDLLAAATVEKLTSVEE
                     IGDRIARSVKDFFENPGCADFVNRLRAHGLQFQLSEEALAARTDKLAGLTVVISGTFE
                     LHSRDEYKAMILQHGGKNSGSVSKNTDYILAGENMGPAKLEKAEKLGIKVIDEKTFLK
                     MLDCSFL"
BASE COUNT         2035 a         1533 c         1903 g         2270 t
ORIGIN      
        1 ctggaatatt acgatgcgga tacccttacg ggtattattc tccgctcagc caacatcttg
       61 aatatcgctt gtgcccgaaa tgcggctgaa gagatcgctt cgagaagccg cggtactccg
      121 aggattgcca acgcgcttct tcgccgtgtc cgcgatttcg cccaggtgaa gggatcgggt
      181 aaaatagaca aggccatttc gtcctatgct ctggaagcgc tgaatataga ccgctacggg
      241 ctcgatgaga ttgacaataa aatacttctc accattattg ataaattcaa aggaggtccc
      301 gttgggatta cgaccattgc tacggccgta ggggatgatg ccgggaccat cgaagaggtt
      361 tacgaaccgt ttctcatcaa agaaggattc cttaagcgta cgccgagggg acgtgaagct
      421 acagaacttg cttaccggca tttgggacga aaaagggacc tggagcaggg tttgttgttt
      481 tgagaatcaa ggctgtaggt gatctagctg ttggagaatg gtttttattt gaccgctttt
      541 ataaaaaaca acgggctgcc aatgaaaatt tacagcccgc agtcttatat cttcttgact
      601 ctcttgtctt aactaaaaag agctgtagat aatgtccagt ttcagttttt ccggtttatt
      661 ctcaagcatt accgccgacg gtttcatctg gttgtaaata gctgtagggg tacttgtccc
      721 cgatgaataa taggagctgc ctgtcgtggt gaaagtaatg tctaccggga ccaggtaata
      781 ggtgaggtcg aacgcttcct tcttctgtct tttgtagtag ttaatcattg ccgcaatatt
      841 cccgaaattg taggaataag tctctttgtc aaaagaagcg taaaaactgg ttacgttgtc
      901 gtgcagtctt ctgttttcaa agaacccttt cagcgaatcc ttattgacca gaagcaagtg
      961 gtcgggagga gataatttca ccttctcatc ctggttcgct tcgggcaagg cgtaaaccat
     1021 gagtcgcgcc tgatttagcg atcggttggt aagttttgag tatatttcgg atatggggaa
     1081 agtgacttct gtgtttactc ctgcggggct ctttacgaaa gtgcccttgt cgttcggagc
     1141 tagcaactgg tcgttgttgt tttgtatgtg attgatttgc gtcacttcgg gagtaatgtt
     1201 caaccggaac tcggaggtgc gtatcgtatc tgttttttgt gaagaacctt ttggatcgag
     1261 gtacttgtag aaaatgttca gggatgtcag gtttacgttc aggatcgtgc tgttaccaaa
     1321 gtttgtcgtg atgtataaac ccgggaaaaa tttcctgaaa gtatccgtat ttttcagttc
     1381 accgtgatcc ggttttttaa attcatccag aaacgattgt cctatagatt tgggcaactc
     1441 cgtataaatc tcgtaaatct tgacggtttg ggtggtgtta cctgtataat atgtttctgt
     1501 acggtaggtg ttgtttttgc cggtaaacgt ttttgtaccg agcgggttgc tcatgtccga
     1561 tttcgtcctg ggatcgaagt tggtgtaatc cttattttta ggcagttcct caatgacctt
     1621 gtatacggcc agtctcatgg gtgccaggga atcaccgatt atggaggagt aggaaactgt
     1681 cagccgtacg gaatcaatga tagcgccatc tttaaacgac tgattttcgg gataataaaa
     1741 ttcacctacg tattcagacc ggatagaacc gaaaaccgga tcaatatatt ctcccagaat
     1801 ggggtatttg gttctggaaa aaacagaatc cacttgtatg gtccgggatg aaacatacaa
     1861 cgtgtccgta cccactgaaa ccctgtcgtt ttccggttga atggtaaatc ctattttatc
     1921 cagggtgtca acgcaggaag cgagaacggt aacaaaagca atgaataata gtttgtataa
     1981 ggtttgaagt ttcattcgtt tcaaaaaatt atgaacgcaa atatctgaaa aaaatatctg
     2041 aaaaaaaagc ccttaacggg ctttcttgtt ttttttaatg ataattgtct atcagaacaa
     2101 aaaacacctg cttaatggat ttttttttaa tacaatttag gcgtcgatct tatcgcaaaa
     2161 ttccccgatt ttctccacgt tattgtcaaa atcgggagta tatcccaagt agggtatttt
     2221 tttatcgatg atatacttgt ccaactcttt atgaatattt tcactgcctt gaacaactcc
     2281 gtccgaataa tcgatggcca gttttgtgag gtttacgaaa tccattttac ctcgtttttt
     2341 cacatcttcg atctctttgt ctccaatggt atcgaaacga agtttttctg aaaaactttc
     2401 gttaaaaggt ttttcgaagt tctcgtcgta aacggaataa ataactttgg cattctttaa
     2461 gcagggatcg tctttgtatc ctttctttat ataaataggc gccaatgcgg taaaccagcc
     2521 gtggcaatga atgatgtcgg gaacccaacg cagttttttg atggtttcca atacgccgcg
     2581 gacaaaaaag atgcttcgtt catcgttgtc atcatattcc tctcccttct gatcagtaag
     2641 cgtctcacgg ttttgaaaga aatcgtcgtt atcgatgaag taaacctgca tgcgagccga
     2701 ttgtatggaa gccaccttga taatcagcga atggtccgaa tcatcgatga tcaggttcat
     2761 tccggaaagg cgaatcactt cgtgcagttg attcctacgc tcgttgatgt tgccgaattt
     2821 aggcataaac gtacgaattt cgtggccttt atcctgaatg gcttggggga ggtatctcga
     2881 aatatgcgag atttgtgttt ccggcaaata aggaaaaatt tcttgggtaa tgtagagaat
     2941 tcttttttga ctcatttaat taattttctt caaattcttt acaaagataa taaaaaaaat
     3001 ccgcataact gcaaaatgtt ttcagctgtt ttttatttat ttttgatttt cagttattta
     3061 tgtaagtgga agtccatatg gattgcgtaa atgaattgac gtttctcttt tttattccgc
     3121 aaaaaattcg ttgctttgca cgctgaaata aatgacggaa agaaaccggt gtattccaat
     3181 aaacatgtag taaaaataag tttacctatc ctgctcagtt tgctggcgca gaatatcatt
     3241 caggtaatag atactgcctt tctcggccgg gtgggggagg tggagttggg cgcatctgct
     3301 ctagccggga ttatttacat tgccatttac acgattggtt tcgggttcag tatgggaagt
     3361 cagatcttga tcggcaggag aaacggagaa aaaaaattcc accaaatcgg agagattgtt
     3421 attcagggga ttttatttct tcttgttccg gcattgttgt tgatcgcggt atttaaactc
     3481 gggttcaccg ataccctgat gcgcttgttt gaatcggaaa atgtgagtga ggctgtttcc
     3541 gattatctcg actggcgcgt ctatggcttt gttttcgctt ttgcaaacag cacctttcgt
     3601 gcgttttata ttggtattgc ccgtaccaag gtccttacga taaattcact ggtcatggct
     3661 ctggtgaatc tggtgctcga ctatggactg attttcggaa acttcggact gcctgaaatg
     3721 ggaattggcg gtgctgcgct ggcatcggtt attgccgaag cgtcatccac attgtttttc
     3781 attgtttaca cccggaaaac agttgacctg gagaagtacg gcttccggag aattacgttt
     3841 aagtggtcag tagtaaaaaa agtgttggat atttccatat atatgatgct tcagtatctg
     3901 ttatccatcg tgacctggtt gatgtttttt gtgtttatag aaaactacct gggtgagcgt
     3961 tctttagctg tgaccaatat tgtgcgcagc ttatacacca tcgtcaccat tccgtctcat
     4021 gcgttgggtt cggcagtgaa caccatggta agcaatacta tcggcgccgg gcggcagaat
     4081 gaagtgctga atttgatcaa gcgggttacc cttatcagtt tgttttcgat gcttgccatc
     4141 attctcatcg tagcggttgc accccgcctg atgattcata tttatacgaa cgatacttct
     4201 cttattgacg atacggtagc gccgttgtac gtgctgttga cctccctgcc tttttatgca
     4261 atagggacgg tgttgttcag tgcagtttcg gggacaggaa atacgcagcg ggcattgttt
     4321 tatgaaatca tcactttatc ggggtatgtt ttatatacat ggtttattgt tgtttacctt
     4381 cggttgtcgg tcggttgggc atggacaacc gaacacgttt actgggggca actgatgttt
     4441 ttttctctgt tttatttgcg aagcaagaag tgggtacata agaagatata gggaattttc
     4501 cttatcttta ccgtcgaaaa acgaacaata tgaaacacag gtttctcttc tttctagtgg
     4561 tgtttttttg ttcttcggtt tatgcccagg aatatcggtg gaaagtgggg ttagactatt
     4621 ttttcgataa tacggaatac gggaaatcgt cgtttaccga ttccgaaacc atgaatggaa
     4681 tctggcttaa tccgatggga agcatcggtt ggggagaaaa acactccatt cgtgccggcg
     4741 taaatctgct gaaaatccca gggatggata aaattgtcga taaagtggaa gtctccttgt
     4801 gttatcaata taaatcgccg gctatggtgt ttcgcgccgg ttcttttccg cggagcgaag
     4861 tgttgggaaa ttacaacaat ttctttttta aggattcagt gaatcatttt gtgccgttaa
     4921 tgcagggagt gttttggcaa atcggaagca accggaattt tctcaacatg tggttcgaca
     4981 gaacaggata tgccacacgc accaggcacg aacatttttt tgtaggatta tcaggaaaaa
     5041 tatccaatgg acttctcttt gccgattttc agtcgtacat gtttcactac tccaacacaa
     5101 agcctgcgac gccgggtgaa ggtgtgagcg aaaatctgca gctgcaggcg acaaccgggc
     5161 tggattacga aggagagggt gggtttaaag gattaatcgc tgtgggaaca ttgatgggat
     5221 acgaaagaga tcgtcggttc gaaagcgaac ggtacaagcc tgtcgggttc gttgcccgcg
     5281 gcgatgccga aatgtgggga atcggtacaa aaaatacgtt ttaccggggg gagccgcgca
     5341 tgagattggc cggtaccttt ggagggaacc tctattgggg aacacaattc cttcgggcaa
     5401 agtcgtatgc cgaaagtgaa tggtacatcc gcctgataga gtcggatttt gtaaaggcac
     5461 ggttcaactg caacctgcat ttcaccgaag gcaacgtgtt gtttcagcag atgcttaccg
     5521 tttctgcatc gatcggtaat tttacaccaa agaataggaa aacaacccgt tatccttgga
     5581 tgagaatatt tcattagaat caagaatcaa gataaaagat attttaatga aggatttgtt
     5641 ttcaaataac aacatacaac ctgcagaatc tccatcggtt ggcggaaatt cggcggctaa
     5701 agataagttg gaaaccctcc gcaaacagtt acacgagcat aattacaatt actacgtact
     5761 gtcgcaacct accatttccg atattgattt tgatgcattg atgcagcaac tggcggaatt
     5821 ggaaaaggag tatcctgagt tttacgatcc caattcgccc acgatgcggg tgggaagcga
     5881 tattaacaag aactttgttc aggtgcagca tcgatatccg atgttgtcgc tgcaaaacac
     5941 ttacaccgaa gaggagattg ccgatttttt caatcgtgta aagcgctcac tgaacgagga
     6001 ttttgaaatt gtatgtgagt tgaaattcga cgggacatcg atttcactcg tttacgaaaa
     6061 tggcaggtta tcacaggcta ttacccgggg cgacggcaag caaggcgatg atgttacggc
     6121 taatgtccgt accgtgcgaa acatcccgtt ggtgctcaaa ggtgacaata tccccgggta
     6181 tgttgaagtg cggggcgaaa tcttgatgcc ttggagtgtt tttgaggaac tgaacaggga
     6241 gcgcgaggtg caggaggaac cgctttttgc caatccccgc aatgcggcat cgggaacact
     6301 taaaatgcag gactcaaagg tggttgcagc caggaaactg gagtcgtctg tgtattacct
     6361 gatgggagaa ggattgccgt cagacagtca tttcgaaaat atggaacttg cccgcaaatg
     6421 ggggctgaat gtatccgcta caatgaaaaa gtgttgttcg ttggaagagg ttttcgagtt
     6481 tttgaaatac tgggacgtgg cgcgtaaaag cctttctgtt gctaccgatg gcgtggtgct
     6541 gaaagtagat tccctttcgc agcaacgcaa tctcggctca acctccaagt tcccccggtg
     6601 ggccattgct tacaaattca atgccgagaa ggcccttacc cgtctggagt ccgttactta
     6661 ccaggtggga cgcaccggtg cagtaacacc cgtagccaac cttgaacctg ttttgctttc
     6721 gggaacaacc gtaaaacggg cttccttgta caacgaagat gccattcttg cacttgatct
     6781 gcacatcggc gacagggttt acgtggaaaa aggcggtgaa atcataccca agatcacagg
     6841 agtggataaa gaggcccgtt tccttatcgg ggacaaggtg cggtttgtca cccgttgtcc
     6901 cgattgcgac actccgttga tacggaacga ggatgaggcc gtgcactact gccccaatag
     6961 cgaaaattgt ccgccacaga taaaaggacg tatcgagcat tttgttaccc gtagggcaat
     7021 gaacatcact atgggcccgg aaaccatagg gctgctttat gataaaggcc tgatcaggga
     7081 tgctgccgat ttatacgcgt tgcaattcga ggacctggtg agcctggagc gttgggcgga
     7141 aaccagtgct aataacctgc tggcaagcat tgaaaaatcg aagacagtcc cttacgaaag
     7201 ggtattgttt gctctcggaa ttcgttttgt gggagagacc gttgctcaga agctggcatt
     7261 ggcctttcac gatatcgatt tgcttgcagc tgcaacggtg gaaaaactaa cgtcggtgga
     7321 ggaaatcggc gaccgcattg cccggagtgt aaaagacttt ttcgaaaatc ccggatgcgc
     7381 ggattttgtg aacagacttc gtgcgcatgg cttgcaattt caactgagtg aagaagccct
     7441 ggcggcaagg acggataagc tggcgggact taccgtggtg ataagcggaa catttgagtt
     7501 acattcgcgc gacgaatata aggcaatgat tttacaacac ggaggaaaga acagcgggtc
     7561 ggtatctaaa aatactgatt acatccttgc cggagaaaat atggggccag caaaattaga
     7621 aaaagccgaa aagctgggga tcaaggttat cgatgaaaaa acatttttga aaatgttgga
     7681 ttgttcattt ttataatatg gttattactt tcattatatt gattgcaaca gcgtctctgt
     7741 t
//
LOCUS       DAOSDR010000111         7711 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrimonas sp. isolate AMR_MDS_1826
            NODE_3493_length_7711_cov_4.071055, whole genome shotgun sequence.
ACCESSION   DAOSDR010000111 DAOSDR010000000
VERSION     DAOSDR010000111.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863355
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrimonas sp. (wastewater metagenome)
  ORGANISM  Petrimonas sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Dysgonomonadaceae; Petrimonas.
REFERENCE   1  (bases 1 to 7711)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7711)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:10:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,720
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 35
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7711
                     /organism="Petrimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3493_length_7711_cov_4.071055"
                     /isolate="AMR_MDS_1826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2023866"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..206
                     /locus_tag="PLI30_05775"
     CDS             <1..206
                     /locus_tag="PLI30_05775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI79099.1"
                     /translation="PYRQTVLLVREIHMLFSYGCYRLIFFIKVVMTIERSGRLVDSHP
                     VVEGGVQADLRASVIRISLKFSV"
     gene            complement(233..1888)
                     /locus_tag="PLI30_05780"
     CDS             complement(233..1888)
                     /locus_tag="PLI30_05780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007654111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HOI79100.1"
                     /translation="MLEKFLKQISFSSHQDFVDNYAIQVPENFNFAYDVVDEWAKTHP
                     TKRALCWTNDKGQHKDLNFSQLKKLSDKTASFLLSLGIRKGDMVMLILKRNIEFWHVI
                     LALHKIGAVAIPATHLLTDKDIVYRANAADIKAIITSQDDNLVEHVNGAMPKSPSVKY
                     RIMIGNNIPEGWIDFHNGVKNAVPFVKPEQVNKNEDIMILYFTSGTTGQPKMVIHDFT
                     YPLGHITTAKFWHNLHENSIHLTLADTGWAKAVWGKLYGQWIVGACVFVYDFEGRFIP
                     ADVLSMISKYRVTSLCAPPTIFRFLIREELDKYDLSSLEYCTTAGEALNPSVFETFYR
                     KTGIKMMEAFGQTETAPTVITFPWIEPKPGSMGMPNPLYKMDLLTYDGRSAEDGEQGE
                     IVFRTDEGRPLALFMGYYRDEELTRSVYTNNLYRTGDMAWRDEDGYYWFVGRTDDIIK
                     SSGYRIGPFEVESAVMTHPAVVECAITGVPDEIRGQVIKATIVLAPQYKEKAYDDLAH
                     DIQEHVKNVTAPYKYPRIIEFVNELPKTISGKIRRVEIRRKDS"
     gene            complement(1960..2520)
                     /locus_tag="PLI30_05785"
     CDS             complement(1960..2520)
                     /locus_tag="PLI30_05785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019539724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HOI79101.1"
                     /translation="MTTDIKAIGERIKGLRDALDFTPGEMAQRLELETSVYLAYEEGK
                     NDISISLLKRIEKEFNVDLATLMFGTEPRMTSYFVTRKDQGLAVERVAAYKYQSLTAG
                     FTHNMGDVFIVTVEPKAKDTDFFLNIHPGQEFNLVLEGSLLFHHNGKNITLNEGDSIY
                     FDSSLPHGMKALNNKAVKFLAVVIEP"
     gene            complement(2588..3385)
                     /gene="zupT"
                     /locus_tag="PLI30_05790"
     CDS             complement(2588..3385)
                     /gene="zupT"
                     /locus_tag="PLI30_05790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006800118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZupT"
                     /protein_id="HOI79102.1"
                     /translation="MDNQVVLTAFLLTLFAGLSTGIGSLIALIAKRTNTNFLSFALGL
                     SAGVMIYVSFMEMLPKALEMLSESFGEKIANLYMLLALFGGIAFIALIDFLVPKSSNP
                     HEMHGVEEMKKNKLRLKHTGIITALTIGIHNFPEGIATFMTAINSLEVAIPITAAIAI
                     HNIPEGIAVAVPIYHATGSRKKAFWLSFSSGLAEPVGAIIAYLFLIRFWNPVLDALIL
                     AIIAGVMIYISLDELLPSAEKYGKHHLSIIGLVTGMVIMAVSLYLFI"
     gene            complement(3392..4192)
                     /locus_tag="PLI30_05795"
     CDS             complement(3392..4192)
                     /locus_tag="PLI30_05795"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012673.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008934 - inositol monophosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006020 - inositol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046855 - inositol phosphate
                     dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HOI79103.1"
                     /translation="MIDINKIRNNVEQIACSTGEFLKEQQAQLHQSDIELKGTRNFVT
                     HIDTEAEKMLVKKLGELIPGATFLTEEGTVTYVDGQYTWIIDPLDGTTNYVHGDTPFS
                     VSIALMKDREIILGVVYDPVANELFSASAKGKVTLNGNSFKTSLHQTLTNGYIGFGIP
                     YSLDDRGEKILKNAAGQFRKCSFRIKGSAAIEICYVACGRSDAYFHSGLSPWDVAAGT
                     FILECAGGTCTDFSGGSNYIFNKELVASNGKIHQEIMDKIINNGETNP"
     gene            complement(4227..6761)
                     /locus_tag="PLI30_05800"
     CDS             complement(4227..6761)
                     /locus_tag="PLI30_05800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006842615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HOI79104.1"
                     /translation="MRYNLLLITLSVLLPLSAQRNFMPSGIHSFVPITVQRPVLLDST
                     DLKDSKFSDETLLSYSISFPEQERFTTLLEPDTAGFFHLPKPENGYAFQLLSFFVTGD
                     RYGKGKLTVTSPNPLELYVNDVKRATKTQVNDSLHQSGSVDAFLNGFTNNARIIIKLL
                     ASAENKIDPAVKIELKPDKGDSLLNFSFNTIDKRRISINDILEGTRVNSSSVSPSGRF
                     VLLSLRETIKGGENRNYTEIYDTRQKRTVLSETANRSQLNWMPKSDILYYVADNNDGR
                     TMYTLNPLTSETKIIAQQLPKENFHVAPDEKSMFYSSKETLSVTTPSGLRRLIGIDDR
                     QAHYRDRFYLYRYFFETGFAQQLTFGRQTATLNDVTDDVKYLLFSTSGEDLSQRPFSK
                     SSLYMLNLETMAVDTVWKDQTYTYSAQFSPDGKQLLIHGAPEAFNGIGLNVKEGQIAN
                     SYDTQSFIMDLATRRVDPVTKNFDPAINAQEWNSADHSIYYRVEEGDRANVYRYSPKT
                     KKFDKLPLKEDVIRSFSISGNGRWAAYTGVSVSNSNRSYLLDLKTNESTLISDPYAKQ
                     LNTLVLGKVKDWKFTSSFGDEIEGRYYLPPHFNPNRKYPLIVYYYGGTSPTSRGFEST
                     YPLHVYAAQDYVVYTLQPSGTTGYGQEFSARHVNAWGDQTAEEIIEGVQKFVAEHPFV
                     DGTKIGNIGASYGGFMTQYLITRTDMFAASVSHAGISNITSYWGEGYWGYSYSAGASA
                     GSYPWNNPKLYTEQSPLFHADKIKTPLLLLHGTADTNVPIGESIQMYTALKLLNKPVE
                     FIQVEGENHAIYDYDKRIAWNNAIYAWFAKWLKGDSRWWDSMFGEK"
     gene            complement(6758..7570)
                     /gene="ispE"
                     /locus_tag="PLI30_05805"
     CDS             complement(6758..7570)
                     /gene="ispE"
                     /locus_tag="PLI30_05805"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019539720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HOI79105.1"
                     /translation="MICFPNAKINLGLNIISRRGDGYHNLETLFYPIGLRDGLEIVPS
                     GREEDFRLFQTGATIQGDMDDNLVIKALKLISEEKKIPGIDIHLFKKIPFGAGLGGGS
                     SDAAFMLKLLNDTFSLGYTVPELEQKAAEIGADCPFFIKNKPAFAKGTGNLLEEVELD
                     LSSYFFVLVKPDITVSTKEAYSLISPMPPEIPLGEIIQKPVSEWKGLLKNDFEPPIFK
                     KYPEICRIKEQLYAKGAVYASMSGSGSSVYGFFEKETDIRFDNCWVWKNKEL"
BASE COUNT         1894 a         1742 c         1844 g         2231 t
ORIGIN      
        1 tcccataccg acaaactgtg ctccttgtcc gggaaataca tatgcttttt tcatacggtt
       61 gttatcgttt aatttttttt attaaagtgg ttatgacaat tgagcgaagc gggaggctgg
      121 ttgactccca tccggtcgtt gaaggtgggg tgcaagctga cttacgtgct agtgtcataa
      181 gaatatcatt aaaattttct gtttaacgtt tcaataggca ttttgttttg gatcaactgt
      241 cttttctcct aatctccacc cgtcggattt ttccacttat ggtttttggc agttcattta
      301 cgaattcaat gatgcgtgga tatttgtagg gtgcggttac gtttttcacg tgctcctgaa
      361 tatcgtgcgc caagtcgtca tatgctttct ctttgtattg tggtgctaaa acaatggtgg
      421 ctttgatcac ttgtccgcga atttcatcgg ggacaccggt aatggcgcat tccacaacag
      481 cggggtgtgt cataacggcg ctttccactt caaacggacc aattcggtaa ccggaacttt
      541 tgatgatatc gtcggtccgt cctacaaacc agtaataacc gtcttcgtcg cgccaggcca
      601 tatcgccggt acggtaaagg ttattggtat atacgctgcg tgtgagttct tcatcgcgat
      661 agtatcccat aaacagagcc aatggacgtc cttcatcggt acgaaataca atttcaccct
      721 gctcgccgtc ttctgctgaa cgaccgtcgt atgtaagcaa atccatttta tagaggggat
      781 taggcatacc cattgaaccg ggtttaggtt ctatccaggg gaaagtaatg acagtgggag
      841 ccgtttcggt ctgcccaaaa gcttccatca tcttgattcc ggttttccgg taaaatgttt
      901 cgaagaccga aggatttaac gcttcccctg cagtggtaca gtattccagt gacgataaat
      961 cgtatttgtc cagctcctcg cgaatgagaa acctgaagat agttggtgga gcacaaagtg
     1021 aggttactcg gtatttcgaa atcatcgaga gcacatcggc agggatgaac cgtccttcaa
     1081 aatcgtacac aaacacgcat gcacccacaa tccattgccc gtagagtttt ccccacacgg
     1141 ctttcgccca tccggtatcg gctaaggtca ggtgtatgct gttctcgtgc aggttgtgcc
     1201 agaattttgc ggtggtgatg tgcccgaggg gataggtaaa gtcgtggatg accattttgg
     1261 gttgtcctgt ggttcccgac gtgaaataga gaatcatgat atcctcgttc ttgttcacct
     1321 gttcgggttt cacaaacggt acggcattct ttacgccgtt gtgaaagtca atccaccctt
     1381 cgggaatgtt gtttccaatc ataatccggt attttaccga cggtgatttt ggcatggccc
     1441 cgtttacgtg ctcaaccaga ttgtcgtctt gtgaagtaat tatcgccttg atgtcagctg
     1501 cattggcgcg ataaacaatg tccttgtcgg taagcaggtg tgtggcggga atagccacag
     1561 ctcctatttt atgaagagcc aggataacgt gccagaactc gatgttccgt ttgagaatca
     1621 acatcaccat atcgcctttc cgtatgccca gggaaaggag gaacgatgcc gttttatcgg
     1681 ataacttttt taactgggaa aagtttaagt ctttgtgttg gcctttatcg tttgtccagc
     1741 acaaagcgcg tttggtggga tgtgtttttg cccattcgtc cacgacatcg taggcgaaat
     1801 taaaattctc gggaacttgg attgcatagt tgtctacaaa atcctgatgc gatgaaaacg
     1861 agatttgttt taaaaatttt tctaacataa aattctgctt cacgtaattt gttcgcgtaa
     1921 ctaattagct gcacgttata ggttgcgttg ttgatcagcc tatggctcga taaccacagc
     1981 caggaatttc actgctttgt tgttcaacgc tttcatccca tggggtaatg aggaatcgaa
     2041 ataaatgctg tcgccttcgt ttaatgtgat gtttttaccg ttatgatgga aaagtaggct
     2101 tccttcgagc acgaggttga attcctgtcc gggatgaata tttaagaaaa aatcggtgtc
     2161 ctttgctttt ggttctaccg ttacgatgaa gacgtcgccc atattgtggg tgaatccagc
     2221 cgtcaacgac tgatatttat atgctgcaac gcgttcaaca gcgaggcctt gatccttacg
     2281 ggtcacgaag taggaggtca ttcttggctc cgtaccgaac atcagcgtag ccaggtccac
     2341 attaaactcc ttctcgatac gcttaaggag cgatatggaa atgtcgtttt taccttcttc
     2401 ataggctaga tagacacttg tttcaagctc aaggcgttga gccatttcgc cgggcgtgaa
     2461 atccagggcg tcgcgcaatc cttttatgcg ttctccaatt gcttttatat cggtagtcat
     2521 gtctgatgtt gttaaagtgt gtatagatag tgtacaaagt taatcaattt ctcagcaatc
     2581 gtgcttgtca aataaataaa tataaactga ccgccataat aaccattcct gtaaccaggc
     2641 caatgatcga gaggtggtgc tttccgtatt tctcggcact cggaagtagt tcgtccaacg
     2701 atatatatat cataaccccg gcaattatag ccagaataag tgcatcgagt acgggattcc
     2761 agaacctgat cagaaagagg taagcaataa tggctcctac cggctctgcc agacccgatg
     2821 aaaacgataa ccaaaaggct ttttttcggc ttcctgtagc gtgatagatg ggaacagcaa
     2881 cagcaatgcc ttccggtata ttgtggatag caatggctgc cgtaatgggt atagcgactt
     2941 ccagcgagtt aatcgctgtc ataaacgtag caatcccttc ggggaaattg tggatgccaa
     3001 tagtgagtgc ggtaatgata ccggtatgtt ttaaccgcag cttgtttttt ttcatttctt
     3061 ccacaccgtg catctcatgg gggttgctcg atttgggaac caggaagtcg ataagcgcaa
     3121 taaaggcaat ccctccaaaa agtgccagaa gcatatacag gtttgctatt ttttcgccaa
     3181 aggactccga tagcatttcg agcgccttgg gcaacatctc cataaatgaa acgtaaatca
     3241 ttacccctgc cgacaaccct aaggcgaagc tcaggaaatt ggtattggta cgcttggcga
     3301 tgagcgcaat aaggcttcca atgccggttg aaagtccggc aaacagcgta aggagaaatg
     3361 cggtaagaac tacttggtta tccatgtcgg gttatgggtt agtttctccg ttattgatga
     3421 ttttatccat tatctcctgg tggatctttc cgttgctggc caccagttct ttgttgaaaa
     3481 tataattgct gcctcccgaa aaatcggtgc atgtgcctcc ggcacattcc aggataaaag
     3541 ttccggccgc cacatcccaa ggtgagaggc ctgaatggaa ataggcatcg cttcttccgc
     3601 aggcaacata acagatttca atggctgccg agcctttgat ccgaaaactg cattttcgga
     3661 attgtcccgc ggcgttctta agtattttct ctccccggtc atcaagggaa taagggatcc
     3721 cgaacccgat gtatccgttc gtaagcgttt ggtgcaaact ggttttaaag gaattcccgt
     3781 tcagggtaac cttacctttg gcagaagcgg aaaacagctc gttggcaacg gggtcgtaaa
     3841 caactcccag aataatctcc cggtccttca tcaatgcgat acttacagaa aacggagtgt
     3901 caccgtgaac gtaattggtg gttccgtcca gcggatcgat gatccaagtg tactgtccat
     3961 caacgtaagt gacggtgccc tcttcggtaa ggaaagtagc gccgggaatc aattcaccca
     4021 attttttcac caacattttt tcggcttccg tatcgatgtg cgttacgaaa tttcgcgtac
     4081 ctttcagttc aatatccgat tgatgcaatt gtgcttgctg ttctttcaga aattctcccg
     4141 ttgaacaggc aatctgttca acgttattgc gtattttatt gatgtctatc atctttattt
     4201 agattcttca cattagcttt ataaatctat ttctctccga acatcgagtc ccaccaacgt
     4261 gaatcacctt tgagccactt ggcgaaccac gcgtaaatgg cgttgttcca ggcgatgcgt
     4321 ttatcgtaat cgtagatagc gtgattttca ccctccacct ggataaattc caccggcttg
     4381 ttcaacaact tcagtgccgt atacatctga atactttcgc cgatggggac attcgtatcg
     4441 gcggtaccgt gcaacagtag cagtggggtt ttaattttat ccgcatggaa gagcgggctt
     4501 tgttccgtgt aaagcttcgg attgttccag ggataactgc ccgcacttgc tcccgcgctg
     4561 taggaatatc cccaataccc ttcgccccaa tagctggtaa tgttactgat tcccgcgtga
     4621 gaaaccgatg cggcaaacat atcggttcgg gtaatcaggt attgtgtcat gaatccgccg
     4681 tatgaagccc cgatgttccc aatttttgtg ccatccacaa acgggtgttc ggcaacaaat
     4741 ttttgtacgc cttcaatgat ttcttccgcc gtttgatcac cccaggcatt tacgtggcgg
     4801 gcagagaatt cctgtccgta tccggtggtt ccgctcggtt gcagcgtata aacaacgtag
     4861 tcctgtgccg cataaacgtg caacggataa gtgctttcaa aaccgcggga ggtagggctt
     4921 gtgcctccgt aataatatac gataagtgga tacttcctgt tcggattgaa gtggggaggg
     4981 aggtagtacc tgccttctat ttcatcgcca aaagaactgg tgaatttcca atccttcact
     5041 ttgcccagga ctagggtgtt gagttgtttg gcatatgggt cggaaatcaa tgtagattcg
     5101 tttgttttca ggtcaagcag gtaacttctg ttggaattgg aaacactcac cccggtataa
     5161 gcggcccacc gtccgtttcc ggagatgctg aacgaacgga taacatcctc tttcaaggga
     5221 agtttgtcga acttcttggt tttgggcgag tagcgataca cgttggcccg gtctccttct
     5281 tccacgcggt aatagatgga atgatccgca gagttccatt cctgggcatt gatagcggga
     5341 tcaaaatttt tcgttacagg atcaacacgc cgagttgcca ggtccataat aaaagactga
     5401 gtgtcgtaac tgttggcgat ttgtccttct ttgacattca atccgatacc gttgaaagct
     5461 tcgggtgcgc cgtgaatcag taactgcttg ccgtcgggag aaaactgcgc cgaataggtg
     5521 tatgtttggt ctttccagac ggtatcaaca gccattgttt cgaggttcag catatacagg
     5581 ctgcttttgc tgaaagggcg ttgtgataag tcctcccccg aagtagagaa aagcaggtat
     5641 ttcacgtcat cggtaacgtc gttcaacgta gcggtctgcc ttccgaaagt gagctgctgg
     5701 gcaaatcccg tttcaaaaaa ataacggtag aggtagaacc tgtcacgata atgggcctgg
     5761 cggtcatcaa taccgataag ccttcttagt ccactaggcg tagtgacgga cagggtctct
     5821 ttggacgagt agaacatcga tttttcgtcg ggagccacgt ggaaattttc tttcggaagc
     5881 tgttgagcaa tgatttttgt ttccgaagtc aacgggttga gcgtgtacat tgtcctgccg
     5941 tcgttgttgt ccgctacata atacaaaatg tcggatttag gcatccagtt gagttgtgaa
     6001 cgattggctg tctccgacaa tacggttcgt ttctgccgtg tatcgtaaat ctcggtataa
     6061 ttacggttct ctccgccttt tattgtttcc ctcaaactga gcaaaacaaa cctgccgctg
     6121 ggggagacag aagagctgtt taccctcgtc ccctccagga tatcgttgat ggagatgcgg
     6181 cgtttgtcga tggtgttgaa ggagaaattc aacagggaat ctcctttgtc gggtttcagt
     6241 tcgattttga cggccggatc gattttgttt tcggccgatg ccaatagctt gatgatgatc
     6301 cgagcgttgt ttgtaaaacc gtttaggaaa gcatcaaccg accccgattg atggaggctg
     6361 tcgtttacct gcgttttggt ggcacgcttt acatcgttca cgtaaagttc gagcgggttg
     6421 ggcgaggtaa cggttagttt tccctttccg tagcggtcac cggtaacaaa gaacgaaagc
     6481 aactggaacg cgtatccatt ctccggcttc ggaaggtgga aaaatcctgc cgtatcgggt
     6541 tccaacagtg ttgtaaatcg ttcctgttcg ggaaacgaaa tggagtagga gagcagcgtt
     6601 tcatccgaaa acttgctgtc tttgaggtcg gtgctgtcca gcaataccgg tcgctgaaca
     6661 gtgatgggca cgaatgaatg gatgccggac ggcatgaagt tccgttgcgc cgaaagcggt
     6721 aatagtacac tcagtgtgat cagaaggagg ttatatctca taattcttta tttttccata
     6781 cccagcaatt gtcaaacctt atgtccgtct ctttctcaaa aaagccgtaa accgatgagc
     6841 cggagccgga catggatgca tatactgcgc ctttggcgta caattgttct ttaatcctgc
     6901 aaatttcggg atattttttg aaaataggcg gctcaaaatc gtttttcagc aatcctttcc
     6961 attcggaaac cggtttttgg ataatctccc caagcggtat ttccggcggc ataggtgaaa
     7021 tcagggagta agcctctttt gtggaaacgg tgatatcggg ttttaccaat acgaaaaagt
     7081 agctgctcaa atccaattcc acttcttcca gcagattgcc tgtccctttt gcaaaagccg
     7141 gtttattttt gataaaaaac gggcaatcgg caccgatttc cgccgctttt tgttccaatt
     7201 ccggtacggt ataacccagg gaaaacgtgt cgttcagtaa tttcagcata aatgccgcat
     7261 ccgatgaacc tccgcccaac cctgccccga aagggatctt tttgaataaa tgaatatcaa
     7321 tgccgggaat ctttttttcc tccgaaatca gtttcagggc ctttatgacc aggttgtcgt
     7381 ccatatctcc ctgtatggtt gctcctgtct ggaagagacg aaaatcttct tcccggcctg
     7441 acggaacgat ttctaatcca tcccttaatc cgataggata aaaaagggtt tccaggttgt
     7501 ggtaaccgtc gccacgtctg gaaataatgt ttagtcctaa atttattttc gcgttgggga
     7561 aacagatcat gcaagtatgt ttatagaggt gcaacttctt cccgggcttc caccatcaac
     7621 cggtatagac cggcaagatg agtgaaatcg tcggcaagca aaagagcgag cgcatcgctg
     7681 taaattcttt cgtccgaaac gggaagcgtc t
//
LOCUS       DAOSDR010000112         7653 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrimonas sp. isolate AMR_MDS_1826
            NODE_3521_length_7653_cov_3.682943, whole genome shotgun sequence.
ACCESSION   DAOSDR010000112 DAOSDR010000000
VERSION     DAOSDR010000112.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863355
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrimonas sp. (wastewater metagenome)
  ORGANISM  Petrimonas sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Dysgonomonadaceae; Petrimonas.
REFERENCE   1  (bases 1 to 7653)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7653)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:10:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,720
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 35
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7653
                     /organism="Petrimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3521_length_7653_cov_3.682943"
                     /isolate="AMR_MDS_1826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2023866"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..3518)
                     /gene="metH"
                     /locus_tag="PLI30_05810"
     CDS             complement(<1..3518)
                     /gene="metH"
                     /locus_tag="PLI30_05810"
                     /EC_number="2.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019538800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HOI79106.1"
                     /translation="MKIKEILESRILILDGAMGTMIQRYKLTEEEYRGEQFRDAAKLQ
                     KGNNDLLSITQPKIIDEIHRSYLAAGADIIETNTFNANAISMDDYGMAHLVREINASA
                     AQIARKAADEFTAQNPRKPRFVAGSIGPTNKTASMSPDVENPMYRAVTFDDLRDVYYE
                     QIDALVENGIDILLIETIFDTLNAKAALFATHEVGKKRGRNIPVSLSVTLSDKAGRTL
                     SGQTIDAFLASVSHANLLSVGLNCSFGASDMKPYVKQLRRVSPFYLSAYPNAGLPNQL
                     GEYDETPEKMALQIREFIDEGLVNIVGGCCGTTPEHIAKYVEIVVDVVPPAPVEQPRL
                     MRLSGLEEFVLTPGINFVNIGERCNVAGSRRFLRLIQEKKYEEALQIARKQVEDGAQV
                     IDINMDDGLLDGVQEMTRFLNLLASDPDISRVPVMIDSSKWEVIEAGLKCMQGKCIVN
                     SISLKNGEVEFLEEAGKVMSYGAAVVVMAFDEKGQADTYGRRIEICERAYRLLVGNGF
                     PPQDIIFDPNVLAIATGMEEHRNYAVDYLESVKWIKSNLPGAKVSGGVSNLSFSFRGN
                     NYVREAIHSVFLYHAVQAGMDMGIVNPTESVLYEDIPAEVKELVEDIIFNRREDATER
                     MMEYAQSIKNKTPEESKEKVLEWRSLPLEERLSHALVKGVGDFMDEDIAEALAVYPRA
                     VDVIDKPLMKGMNVVGDLFGSGKMFLPQVVKAARTMKKAVSILQPVIEAEKSSGAAQK
                     AGKIVLATVKGDVHDIGKNIVSIVLSCNNYEIVDLGVMVPPEKIIETVIREQPDIVGL
                     SGLITPSLEEMAVVAAEMEKAGFRMPLLIGGATTSKLHTALKIEPKYSRGAVVYVKDA
                     SQSPGVVANLLNHETNGNYSQQVRDEYAALRENSTDRKVELVSLEEARENAYGIDWKD
                     FSAEKPWMVGRKVMQNIPVEDIVPYIDWKFFFHGWNLSAKFGSVANSDFTVEGRNAWM
                     AKFKDDEQEKAGEAAKLYDDARRLLDKLVSDNAGYINAVFGIYAAYSDGDDIVAEGVR
                     IPMLRQQKKNDKNEYLSLSDFIAPASSGKKDYVGAFAVTAGDGADELLQVYEDEGDEY
                     LALLLKSLLERLAEAATEWLHHMIRKEYWGFAEDENLSVADMLASRYRSIRPAVGYPS
                     IPDQTMNFVLHDMLRTDEIGISLTENG"
     gene            complement(3537..4478)
                     /locus_tag="PLI30_05815"
     CDS             complement(3537..4478)
                     /locus_tag="PLI30_05815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019538801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside recognition domain-containing
                     protein"
                     /protein_id="HOI79107.1"
                     /translation="MNFLKQILFPSFRKSGSTTIWLLKIILPISLLVRFLDYFGALAF
                     IAQFLDPVFLHLGLPGSTAIIFITSIFLPLYAPLAIIMSMTVTLRELTILALMCQIAH
                     NLPVESAIQAKTGTSFWSMFSLRIVVSILVGVLLNLILPADMGMPLFVKVNAELPTSV
                     ADVLVLWLKGSVQMALLIFFIVTVLNVIYKLLEQNNMIVRLSKAIESVLRFFGLSEST
                     GFLWLIGYIVGLAYGGALMIDQMKEGKVTRSDAELLNYHLAVSHSVIEDNLLFVALGV
                     SVWWILGVRLVVAWLVVWVRRIFLKMKRKAMYSYKNI"
     gene            complement(4475..4927)
                     /gene="smpB"
                     /locus_tag="PLI30_05820"
     CDS             complement(4475..4927)
                     /gene="smpB"
                     /locus_tag="PLI30_05820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005677728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006450 - regulation of translational
                     fidelity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein"
                     /protein_id="HOI79108.1"
                     /translation="MKQAPINIKNKRATFDYELLETYTAGIVLTGTEIKSIRLGKASL
                     VDTYCLLEKGELWVRNMHIAEYFYGTYNNHVARRDRKLLLNRNELRKLTRATKETGFT
                     IVPIRLFINEKGLAKLVIAVAKGKKQYDKRQSLKEKEDKRSMDRMFKK"
     gene            complement(4992..5555)
                     /locus_tag="PLI30_05825"
     CDS             complement(4992..5555)
                     /locus_tag="PLI30_05825"
                     /inference="COORDINATES: protein motif:HMM:NF016764.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YIP1 family protein"
                     /protein_id="HOI79109.1"
                     /translation="MFATIIKTIYCLIASPRKGWRRISDRDTGQQDFINNFLFPVFGF
                     IAIPSFIGGMWLADNGGIHWALKQVVIVVSALFGGFYLVSYALNELFPKFGLGKKLHA
                     AQLFVGYSSVVLYLLFFLIPLLPGAVFLWFAIIYTLYIVYAGAGDFLHMTANKKLSFT
                     VIASLLIVVVPLAIKILLEFMINLLPG"
     gene            5712..6233
                     /locus_tag="PLI30_05830"
     CDS             5712..6233
                     /locus_tag="PLI30_05830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019540256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HOI79110.1"
                     /translation="MNAQQFQHQILCYSDKLYRMARSVLKDESLSQDAYQELMMRLWE
                     KRGQLEIIENQQAFVLTSMRNLCIDWLRKQQPNGEIPANATYPAPGPHQLTEEADTVN
                     TISRMIDLLPEMQRTIVRMKDVEEMEVKEIAEIMSITENAVTVNLSRARKKLREMILT
                     HQQQEKLLYEQHR"
     gene            6217..6741
                     /locus_tag="PLI30_05835"
     CDS             6217..6741
                     /locus_tag="PLI30_05835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI79111.1"
                     /translation="MNNIDNILNNYFEGKTLPEEESRLKEYFRSSTVLPQHEIYKPLF
                     IAFDKEKQIAAPVFEIPAEKNRKKPFPIWKLWIAAASIAAVTLLVVTLFPHKNKVGIP
                     SGDYLVFINGNEVTNPQKAQQYADKMFMQADEIIRTSYKPIVEANVIQTEMDADRIFD
                     DLSQKINYIESVKQ"
     gene            6760..7200
                     /locus_tag="PLI30_05840"
     CDS             6760..7200
                     /locus_tag="PLI30_05840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI79112.1"
                     /translation="MKKILSILFLATVFVAGASAQQLEKLFDKYMEDERFNYVYQKKA
                     SMMDRLNDAGNVDVWVFDKSTTSLGRQMLTLNSADKSLDESFTREVENALKADKYEQV
                     SIVRNGKNRVETYEKNTDKGIAKVSFIKNPGNIMVTLNVYAPRK"
     gene            7305..>7653
                     /locus_tag="PLI30_05845"
     CDS             7305..>7653
                     /locus_tag="PLI30_05845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009018032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HOI79113.1"
                     /translation="MQPTKPYRDFSEFLTQRFPFKVQKISINAGFTCPNRDGSKGRGG
                     CTYCNNQSFSPGYGKPTKSISEQLADGINFFSHKYPEMKYLAYFQSYTNTYDSIQKLI
                     EMYEEALAYPDVVG"
BASE COUNT         2157 a         1897 c         1588 g         2011 t
ORIGIN      
        1 ccgttttccg tcaacgaaat tccgatctca tcggttctaa gcatatcgtg cagtacaaaa
       61 ttcattgttt ggtcgggaat ggacggataa cccaccgctg ggcgaatgct ccggtatctt
      121 gaagccagca tatcggcaac agacaggttt tcatcttccg caaaacccca gtattctttc
      181 cggatcatgt ggtgcaacca ctcggttgct gcttcggcca gcctttcgag caatgatttc
      241 aacaataatg ccaaatactc atcaccttcg tcttcataaa cttgcagcag ctcatcggct
      301 ccatctccgg cagtaaccgc aaaagcgccg acataatctt tttttccgga tgaggctggc
      361 gcaataaaat cactcaggct caagtattcg tttttatcgt tcttcttttg ctggcgcagc
      421 atgggaatac gaaccccttc ggccacgata tcatcgccat cgctgtaagc cgcatatatg
      481 ccaaacacgg catttatata acctgcatta tcgcttacca gcttatcgag caaacgacgc
      541 gcgtcatcgt acaatttcgc tgcttcgccg gctttttcct gctcgtcgtc cttgaatttt
      601 gccatccagg cattgcgtcc ttccactgtg aaatccgagt tagcaacaga gccgaacttg
      661 gcagacaggt tccatccgtg gaagaaaaac ttccaatcga tatagggaac gatatcttct
      721 accggaatgt tctgcatcac ttttcttccc accatccagg gtttttcggc agaaaaatct
      781 ttccagtcga tcccatatgc gttttcccgc gcttcttcaa gtgaaaccag ttctactttt
      841 ctgtccgtac tgttttcgcg aagggcagcg tattcatcac gcacttgttg ggaataattt
      901 ccgttcgttt catgatttaa caggttcgcc accactcccg ggctttgtga tgcatcttta
      961 acatatacca ctgctccccg actgtatttc ggctctattt tcagggccgt atgcaacttc
     1021 gacgtggtgg caccaccgat gagcagaggc atccggaatc cggctttttc catttcagcc
     1081 gcaacaacag ccatctcttc gagtgagggc gtgattagtc cgctcaatcc cacaatatca
     1141 ggttgttcac gaataacggt ttcgataatt ttttcgggcg ggaccatcac ccccaaatcc
     1201 acaatttcgt aattgttgca cgacaacacg atggaaacga tgttttttcc gatatcgtgc
     1261 acatcaccct tcacggtagc caacacgatt ttcccggctt tttgcgccgc tcctgacgac
     1321 ttttcggctt cgataacggg ttgaagtatg gaaacagctt ttttcatcgt tcgggcagct
     1381 ttaaccactt gcggaaggaa catcttcccg gagccgaaca agtcgcctac cacgttcatc
     1441 cctttcatca gcggcttatc aataacatcc acagcacgcg gatataccgc aagcgcttca
     1501 gcgatatctt cgtccataaa atcaccgacc cccttcacga gagcatgact taaccgctct
     1561 tcgagaggca gggatcgcca ctcgagcact ttttccttcg attcttccgg ggttttgttt
     1621 ttgatgctct gtgcatactc catcatccgc tccgtggcat cttccctgcg attgaaaatg
     1681 atatcttcca ccaattcttt cacttcggcg ggtatatctt cgtacaacac cgattctgtg
     1741 ggattgacta ttcccatatc cattccggcc tgaacggcat gatagaggaa aacagagtgt
     1801 atcgcttccc ggacatagtt gtttccccgg aatgaaaaag agaggttact cactcctccg
     1861 ctcaccttag ctcccggtaa attggatttt atccatttca cactctccag gtagtctacg
     1921 gcgtaattgc ggtgttcttc cattcccgta gcaatcgcta gcacattcgg gtcaaaaatg
     1981 atgtcttgag gaggaaaacc gttcccgaca agcaacctgt aggctctctc acaaatttcg
     2041 atgcgccggc cgtaggtatc cgcttgtcct ttttcatcga aagccattac cacaactgcc
     2101 gcaccgtacg acattacctt tccggcttcc tccagaaatt ccacttcacc gtttttcaaa
     2161 gagatggagt tgacaataca cttcccctgc atacatttca ggccggcctc tatcacctcc
     2221 catttcgatg aatctatcat cacgggaaca cgtgaaatat ccggatcgga tgctagcagg
     2281 ttgagaaaac gggtcatctc ctgcacacca tcgagcaacc catcatccat gtttatgtcg
     2341 atgacctgtg caccgtcttc tacctgttta cgggcaatct gcagggcttc ttcgtatttc
     2401 ttctcctgaa tcaggcgcaa gaacctgcgt gagcccgcta cgttacaccg ttccccgata
     2461 ttcacaaaat taattccggg cgtcagcaca aattcttcca ggcccgaaag acgcatcaac
     2521 cggggttgtt ctacaggagc cggtggcaca acatccacta cgatttcgac atattttgca
     2581 atgtgttcgg gagtggttcc gcagcaacca ccaacgatgt ttaccaatcc ctcgtcgata
     2641 aactcccgga tttgtaaggc catcttttcg ggtgtctcat cgtactctcc aagctgattg
     2701 ggcagccctg cattgggata agcactcaag taaaaaggtg aaacgcggcg aagctgcttc
     2761 acatagggtt tcatatccga tgcgccgaac gaacagttca accccaccga aagtaagttg
     2821 gcgtggctta ccgatgcaag aaaagcgtct atcgtttgtc ccgacagtgt ccttcctgcc
     2881 ttatccgaaa gtgttactga gagggagaca ggtatattcc tgcccctttt tttccccact
     2941 tcgtgagtcg caaaaagagc agctttggcg ttgagcgtat cgaaaatggt ctcgatcagc
     3001 aggatatcaa tcccattttc caccaaggcg tcaatttgtt cgtaatagac atcgcgcaga
     3061 tcatcgaaag ttacggcacg gtacatcggg ttttccacat cagggctcat cgatgcagtt
     3121 ttgttagtgg ggccaatgga gccggcaaca aaacggggtt tcctggggtt ttgagccgta
     3181 aattcatcgg cggcttttcg cgcgatctga gccgctgaag cattgatttc ccgtaccaga
     3241 tgagccattc cgtaatcatc catcgaaatg gcgttggcat tgaacgtgtt ggtttcgata
     3301 atatcggctc cagcagccag atagctgcgg tgaatttcat caatgatttt cggttgcgta
     3361 atggaaagca aatcgttgtt ccctttctgc agcttcgcag catccctgaa ctgttcgccg
     3421 cgatactcct cttccgtgag cttataacgt tgtatcattg ttcccatagc accatcaaga
     3481 atgaggatgc gggattctaa aatttctttt attttcatgc ttaacaaaat gtcaaattaa
     3541 atattcttat acgaatacat ggcttttcgc ttcattttta aaaaaatccg ccgaacccaa
     3601 accaccagcc aggctaccac caaccttaca cccaatatcc accagaccga tactcccagc
     3661 gccacgaaca ataaattatc ctcaatgacg gaatgggaaa ccgccaggtg gtagtttaat
     3721 aattcggcat ccgaccgcgt tacttttccc tccttcatct ggtcaatcat caacgctcca
     3781 ccgtaagcca gcccaacgat ataaccgata agccaaagaa atccggtgct ctccgacaga
     3841 ccgaaaaaac gtaaaacaga ttcaatagct ttcgacaacc ttactatcat gttgttctgt
     3901 tccaacaact tataaataac gttcagaacg gtaacaatga agaatatcag caatgccatc
     3961 tgaaccgaac ccttcaacca cagcaccagc acgtctgcaa cagaggttgg cagttcagca
     4021 tttaccttga cgaacaaggg cattcccata tcagccggaa ggatcaggtt aagcagcacc
     4081 ccgaccaata tgctgacaac aatccgtaag gaaaacatgg accagaacga tgtgcccgtt
     4141 tttgcctgga tagcgctttc tacgggtaaa ttatgcgcta tctggcacat caatgccaga
     4201 atagtcagtt cacgcaacgt caccgtcatt gacattataa ttgccaacgg cgcatagagg
     4261 ggaaggaaga tactggtgat gaatataatt gccgtgcttc cgggcaaacc gagatgcagg
     4321 aaaacggggt caaggaactg tgcaataaaa gccaaagccc caaaataatc aagaaaacga
     4381 accagcaacg agatgggtaa aataattttg agcagccata tcgttgtact tcctgatttt
     4441 ctgaaagacg ggaaaagtat ctgtttgaga aaattcattt tttaaacatc ctgtccattg
     4501 agcgtttgtc ttccttctct ttcagcgatt gccgtttatc gtattgcttt tttccctttg
     4561 ccaccgcaat aaccagcttt gccagccctt tttcattgat gaacaatcgt atgggcacaa
     4621 tcgtgaatcc ggtttccttg gtcgcacggg taagctttcg caattcgttc cggttcagca
     4681 acagttttcg gtcgcgccgt gccacgtggt tgttgtaggt tccgtaaaaa tattcggcga
     4741 tatgcatatt ccgcacccag agttcgcctt tctcaagcag gcaataggta tccaccagac
     4801 tagccttgcc aaggcgaatc gattttattt cggttccggt aagcacaata cccgcggtgt
     4861 aagtttcgag cagttcgtaa tcaaacgtgg cccgtttgtt ttttatattt atgggtgctt
     4921 gtttcatttc tttgagacta ttggagatca aatgtcagac ttcagactgt tttgttgagt
     4981 gaatagcttt cttaaccggg caataaattt atcataaatt ccaataatat ttttattgcc
     5041 agaggtacaa caacaataag caatgatgca ataacggtga aactcaactt tttgtttgcc
     5101 gtcatgtgta aaaaatcacc ggcacctgcg taaacgatat acaacgtata aattatagca
     5161 aaccacaaaa aaacagctcc cggcaagagg ggtatcagga aaaacaagag gtatagcacc
     5221 accgatgaat aacccacaaa taattgtgcg gcatgcaact ttttacctaa cccaaattta
     5281 gggaaaagct cgtttaatgc atacgatacc agataaaatc cgccaaaaag cgccgacacc
     5341 acgataacca cctgttttaa cgcccaatgg attccaccgt tatcggcgag ccacattccg
     5401 ccaataaaag aaggaatggc aatgaaccca aaaacgggaa acaggaagtt gttgataaaa
     5461 tcctgttgtc cagtatctct atcagaaatt cttctccatc cttttcgcgg tgaagcaatt
     5521 aaacaataaa tcgttttaat gatcgttgca aacattacct tttgattttg aagtgcaaag
     5581 gtaatgattt cttgcgaaac taattaccgg gaatagaaca tcagtcaggc aggttatttt
     5641 ttttgttacc tttgaaaaaa aatccgccaa ccggtgtaag attttttccg gcttcccgtc
     5701 tatatatgtg tatgaacgct cagcaatttc aacatcaaat cctgtgctat tccgataagc
     5761 tgtatcgcat ggcacgctcg gtattaaaag acgaaagcct gtcgcaggat gcctatcagg
     5821 aattgatgat gcggttgtgg gaaaagcgcg ggcagttgga aataatcgaa aaccagcagg
     5881 cctttgtgct gacatcgatg cgaaacttgt gcattgattg gctgcgaaaa caacaaccca
     5941 acggggaaat tcctgctaac gcgacatacc ctgcacccgg tccgcatcag cttacggagg
     6001 aggccgatac ggtaaacacc atcagcagga tgattgactt gctacccgag atgcaacgaa
     6061 ccattgtccg gatgaaagac gtggaggaga tggaggttaa ggaaattgcc gaaatcatgt
     6121 cgataacgga gaatgccgtt accgtaaacc tgtcgcgagc ccgaaaaaaa ctccgcgaaa
     6181 tgattctgac tcatcaacaa caggagaaac tgctttatga acaacataga taacatactg
     6241 aacaattatt ttgaaggcaa gacattgccc gaagaggaaa gcaggctgaa ggaatatttc
     6301 cggagcagta ccgttttgcc tcagcacgaa atatataaac ccctgtttat tgctttcgat
     6361 aaagaaaaac aaattgctgc tcccgttttt gaaattccgg cagaaaagaa ccgtaaaaaa
     6421 ccgtttccaa tctggaaatt atggatcgct gcagcaagta tcgctgcagt tactctcctg
     6481 gtcgttactt tgtttccgca taaaaacaaa gtcggaatac cgtcaggcga ttacctggtt
     6541 ttcataaacg gcaatgaagt aaccaatccg caaaaggcgc aacaatacgc cgataaaatg
     6601 tttatgcaag ccgatgaaat catccgcacc agctataaac cgattgtaga agcaaatgtc
     6661 atacaaaccg aaatggatgc cgacaggatc tttgacgact tatcgcaaaa aatcaattat
     6721 atagaatctg tgaaacaata atttataaac cctaaaaata tgaaaaaaat tctttcaatt
     6781 ttatttttgg caaccgtttt tgttgccgga gcatcggctc aacaactcga aaagttgttc
     6841 gacaaatata tggaggatga gcgtttcaat tatgtgtatc agaaaaaagc gagcatgatg
     6901 gataggctga acgatgccgg aaacgtagac gtctgggtgt tcgacaaatc gacaacaagc
     6961 ctgggacgtc aaatgctcac gttgaattcg gccgacaaat cgctggacga aagttttacc
     7021 cgggaagtgg aaaacgcgct caaagccgat aaatacgaac aggtttcgat cgtaagaaac
     7081 gggaaaaaca gggtggaaac gtatgagaaa aataccgata aagggatagc aaaagtcagt
     7141 tttataaaaa atcccggcaa cattatggtt acattgaatg tatacgctcc cagaaaataa
     7201 ctctactttt aaatacaaac actttacgga aataggatgc gatatcgtgt ttccgttttt
     7261 tatttttaat tatctttgtc cgtttaacaa ccaaaggtca acagatgcaa ccaaccaaac
     7321 cctaccgcga tttttcagaa tttctcacgc agagatttcc ctttaaagtt caaaagattt
     7381 ccatcaatgc cggctttacg tgccccaaca gggatggaag caagggccgc ggaggatgta
     7441 cctactgcaa caaccaaagc ttcagtccgg ggtatggcaa accgacaaaa agcatatcgg
     7501 aacagttggc tgacggcatt aacttctttt cgcacaagta tcccgaaatg aaatacctgg
     7561 catattttca atcgtacacc aacacatacg acagcataca aaaactcatc gagatgtacg
     7621 aagaagcgct tgcctatcct gatgttgtgg gca
//
LOCUS       DAOSDR010000113         7613 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrimonas sp. isolate AMR_MDS_1826
            NODE_3551_length_7613_cov_3.755755, whole genome shotgun sequence.
ACCESSION   DAOSDR010000113 DAOSDR010000000
VERSION     DAOSDR010000113.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863355
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrimonas sp. (wastewater metagenome)
  ORGANISM  Petrimonas sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Dysgonomonadaceae; Petrimonas.
REFERENCE   1  (bases 1 to 7613)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7613)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:10:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,720
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 35
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7613
                     /organism="Petrimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3551_length_7613_cov_3.755755"
                     /isolate="AMR_MDS_1826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2023866"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..991)
                     /locus_tag="PLI30_05850"
     CDS             complement(<1..991)
                     /locus_tag="PLI30_05850"
                     /inference="COORDINATES: protein motif:HMM:NF024245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HOI79114.1"
                     /translation="MKFWVFHIILAVTLCAKCSFAKSQSNDWRSYLAQLAEEGMDDTT
                     IENMFQELTMLEGNPMNLNRVSREQLEQFPLINFEQAAHIAEFLDKNRPVYTVFELRN
                     VSFLDFKTVELILPFFFAGKAEEEQPTVQEMLSRGRHEVQSRFDKTLQSRAGYGEFAD
                     SILQRYPNRKYRGEDFYTSMKYAFTYRDKIQFGLVGEKDPGEPFLQKNYPVGYDHYGL
                     HLIIRELGKLKTLAVGDYRLSFGQGLILNNDFMISKSWGTNTIIRRTQGPKRHFSTAE
                     SGFFRGAAAVFQAGKITVIPFYSNKRFDANLSRDGEITSFKTDGYHRTPSEIEK"
     gene            complement(1091..3559)
                     /gene="lon"
                     /locus_tag="PLI30_05855"
     CDS             complement(1091..3559)
                     /gene="lon"
                     /locus_tag="PLI30_05855"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019537759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HOI79115.1"
                     /translation="MFQNKLSVLKPEESESNVISFIAGDFLDGSQEIDESSLKESLPI
                     LPLRNTIVFPGSGLPISVGRSKSLKLVRALGKKHRYIGLVCQKDNNVEEPEKEDLYSI
                     GVIAEVIRVIELDKNNLSIIVQAKKRFEWTEFTQTEPYFAANYRILESKRAEKDDKEF
                     SAILDSIRDAMNHMLNILGDPPKELLQTINHESFTGMLVSYSATNLPIENREKQELLS
                     ISDEKEQAYRLLMILNRETQVLELKMNIQMKTREDLNQQQKEYFLQQQIKTIQEELGG
                     NTQQIEIAEFRKKGKTKKWSQEVADIFEKEISKLERLHPQSPDYSVQFGYLQTIVELP
                     WNEYTKDSFNLKNAQKILDRDHFGMEKVKERIIEHLAVLKLKGDLKSPILCLYGPPGV
                     GKTSLGKSIAEALNRKYIRVSLGGLHDEAEIRGHRRTYIGAIPGRIIQNIQKAGSSNP
                     VFVLDEIDKVGNDFRGDPASALLEVLDPEQNFAFHDNYLSIDYDLSKVMFIATANNLN
                     TIPQPLLDRMELINVGGYISDEKIEIAYRHLVPKQLENHGIKKKAFSISKPALLKIIE
                     DYTRESGVRELDKKIAKVTRKIARKIAGNEEYPKVLKPTDVTEYLGMEEYSKDRYQGN
                     DYAGVVTGLAWTAVGGEILFVESSLSRGKGSKLTLTGNLGDVMKESAMLAMEYIHANA
                     DLLDIDPAIFENWNTHIHVPEGAMPKDGPSAGITMITSLASAYTQRKVRNNLAMTGEI
                     TLRGKVLPVGGIREKILAAKRAGITDIILCKENEKDILEIKPEYVAGLNFHYVEDVKQ
                     VLEFALLEEKVENPKNFIVPAEKE"
     gene            3748..4407
                     /locus_tag="PLI30_05860"
     CDS             3748..4407
                     /locus_tag="PLI30_05860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009136558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HOI79116.1"
                     /translation="MKVGTDGVLLGAWADFIDCQSILDVGTGSGLIALMAAQRNSLAK
                     ITAIEMDADAALQARENVDNSRFKERIEVLPVSFRDFFPTVTGKFDSIVSNPPFFVNS
                     LPSPDLKRTGARHMDSLTPAELFKMSRKLLNDTGTLSLIYPFSERDNIMNVSEKENFH
                     LSRETVVLPTRSASPKRILLEFTTRSETETDRNELVIEEKRHVYTPEFIHLVQDFYLY
                     L"
     gene            4489..6096
                     /locus_tag="PLI30_05865"
     CDS             4489..6096
                     /locus_tag="PLI30_05865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016267813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exo-alpha-sialidase"
                     /protein_id="HOI79117.1"
                     /translation="MLLSLGFMQAGDTLRVKQTQVPILIDRIDNALFYISIDAREAVK
                     LTELKLKFSESSNLNEVESLKLYYGGTEAVQRKGQTFYHPVQYLSGFTPGKTREANPS
                     YSVKVVEQKSPGIEVAFSANYPLFPGINYFWISLQMKPTTSLLSKIDVNLVSAKLDGR
                     DAPISFANNTETHRMGVGVRHAGDDGVAAFRIPGMVTSNRGTLLGVYDVRYNNSVDLQ
                     EHVDIGLSRSTDKGQTWEKMRIPVAFGEYGGLPKAQNGVGDPSILVDRKTGTIWIAAL
                     WTHGMGNYRAWHNSQAGMDEKRTGQLILTKSMDDGKTWSEPINITSQVKDPSWHLLLQ
                     GPGRGITMQDGTLVFPIQFIDSTRIPNAGIMYSKDSGQTWKIHNHARTNTTEAQVAEV
                     EPGVLMLNMRDNRGGSRAVSITKDLGKTWTEHPSNRSVLQEPVCMASLIKVDANENVL
                     GKDILLFSNPNTTEGRHHITIKASLDGGLSFPEEYQVLLDEDPGWGYSCLTVIDKETV
                     GILYESSVAHMTFQAVKLRDIIKGSRQ"
     gene            6127..6996
                     /locus_tag="PLI30_05870"
     CDS             6127..6996
                     /locus_tag="PLI30_05870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019541175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HOI79118.1"
                     /translation="MKKYLFIFSLLSTMVMAQETPILLFPDGAPGETTKMKQKDDLSG
                     NKVAGCPVLRISDVSEPTLTFFPAPADNNSGATIIVNPGGGYNILAYNLEGTEICKRF
                     NSHGVNCVLVKYRVPRREGLEKHQAPLQDLQRAIAYTRSHAGEWKIDPNRIGVMGFSA
                     GAHLAAMASTAYDERTYPAVDAADNASLRPDFCVLIYPAYLDGENFSISPELKVTEKT
                     PPTFIVQTQDDHRLLNSSLFYYYALKEAKAPVAMSLYPSGGHGYGLRNTGDLVNEWPF
                     RVMSWLHDIKMVE"
     gene            complement(7005..>7613)
                     /locus_tag="PLI30_05875"
     CDS             complement(7005..>7613)
                     /locus_tag="PLI30_05875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004320523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4143 domain-containing protein"
                     /protein_id="HOI79119.1"
                     /translation="KDMMQFQTFLRLCAARIGSLFNASELSGEVGVSANTVKSWLSVL
                     QASYIIKLLTPFHENMRKRLTKTPKLYFCDTGLACYLLGIETEQQLTRDKMRGHLFEN
                     FIVMEALKNRYNRGKESNLLFYRDSNGVEVDLLFKNGSDYSAVEIKSSQTYHPDFETG
                     IRSLNALLKDRLTDKAVLYAGDFENDTAEIKLFNYKNMYRLF"
BASE COUNT         1882 a         1789 c         1805 g         2137 t
ORIGIN      
        1 ttttctctat ttccgaaggc gttctgtgat acccatcggt cttgaacgag gttatttctc
       61 cgtcgcggga aagattggca tcaaatcttt tgttggagta aaaagggata acggttattt
      121 tccctgcctg gaagacagca gctgcaccac gaaaaaatcc gctttcggcg gtggagaaat
      181 gtcgtttagg tccctgtgtc cggcggataa tggtgttggt gccccaggat ttggagatca
      241 taaagtcgtt gttgagtatt aatccttgtc cgaacgatag gcgataatcg cccacggcaa
      301 gggttttaag ctttcccagt tcgcgaatga tcaggtgcag tccgtaatga tcgtatccca
      361 cgggatagtt tttctgcaaa aacggctcgc cgggatcttt ttcgcccacc aacccaaact
      421 gaattttatc gcgataagtg aatgcgtatt tcatcgaagt ataaaaatcc tctccccggt
      481 atttccgatt gggataacgc tgaagaatgc tgtctgcaaa ctctccgtat cctgcccggc
      541 tctgaagggt cttgtcgaac cgggattgaa cttcgtgtcg cccccgactg agcatttcct
      601 gcaccgttgg ttgttcttcc tccgctttgc ccgcgaaaaa aaacggcagg atcaattcta
      661 cagttttaaa atcgagaaaa gagacattcc ggagttcaaa taccgtgtag accggtcggt
      721 tcttatccag aaattcggct atgtgagccg cttgttcaaa attgatcagt ggaaactgct
      781 ccagttgttc gcgggaaacc cggtttaaat tcatggggtt gccctccagc atcgttagtt
      841 cctgaaacat gttttcgatg gtggtatcgt ccattccctc ttctgcaagc tgtgccagat
      901 aggagcgcca atcgtttgat tgcgacttgg caaaagagca ttttgcacaa agagtaacgg
      961 caagaatgat atgaaaaacc caaaatttca ttcatcactg agctaaaagc cgggattttt
     1021 acaaatataa cctcctcgga acgattttcc caataattga tgaacaaaag ttcgggtaag
     1081 gatagtcgcc ttactctttt tctgccggaa cgataaaatt cttggggttt tccacttttt
     1141 cttccagcag ggcaaattcc agcacttgct tcacatcttc cacatagtga aagttcaaac
     1201 ctgccacata ctcgggtttg atttcaagaa tatctttttc gttctctttg cagaggatga
     1261 tgtcggtaat tccggcacgc tttgctgcca gaattttttc ccgaattcca ccaaccggta
     1321 gtacttttcc ccgcagcgtg atttctcccg tcatggccag attgttccgc actttacgtt
     1381 gcgtataggc tgaggccagt gatgtgatca tggttattcc tgccgatggc ccgtctttgg
     1441 gcatggcgcc ttcgggaacg tgaatgtgcg tgttccagtt ctcaaaaata gcggggtcaa
     1501 tatccagcag gtcggcattg gcgtgtatgt actccatcgc cagcatagcc gactccttca
     1561 tcacgtcgcc caggtttccg gtgagcgtaa gttttgagcc ctttcctcga ctcagcgaac
     1621 tttccacaaa aaggatctct ccccctacgg ctgtccaggc caaccctgta accactccag
     1681 catagtcgtt gccctggtaa cggtctttgc tgtattcttc catgccgagg tactccgtga
     1741 catcagtagg tttcaatacc ttgggatact cttcatttcc agctatcttc cgggcaattt
     1801 tccgggttac ctttgcaatt ttcttgtcca gttcccttac ccccgattcg cgggtgtaat
     1861 cttcgatgat cttcagtaag gccggtttag aaatgctgaa agccttcttc tttatgccgt
     1921 gattttccag ctgttttggg acgaggtgac gataggcgat ttctattttt tcatcgctga
     1981 tgtagccccc aacgtttatc aactccatcc tgtcgagaag aggttggggg atagtgttca
     2041 ggttgtttgc cgtagcaatg aacatcactt ttgaaaggtc gtaatcgatg gagaggtaat
     2101 tgtcgtgaaa tgcaaaattc tgttcaggat caagcacttc gagcagcgcc gatgccggat
     2161 cgccccgaaa gtcgttaccc actttatcta tttcatccag cacaaaaacc gggtttgaac
     2221 ttcctgcttt ttgaatgttc tggataattc ttccgggaat ggccccgata taggtgcggc
     2281 gatggccgcg aatttcagct tcatcgtgca gaccacccag tgaaacgcgg atatacttcc
     2341 tgttcaatgc ttcggcaatc gattttccga gagatgtctt acctaccccg ggagggccgt
     2401 aaaggcacaa tatcggagat tttaaatcgc ctttcagttt caaaacagcc agatgctcga
     2461 tgatgcgctc tttcactttt tccattccaa aatggtcgcg atcgagaatt ttctgtgcat
     2521 tcttcaggtt gaagctgtct ttggtatact cattccacgg caactccaca atggtctgaa
     2581 ggtagccgaa ctggaccgaa taatcgggtg actgcggatg taacctttcc agtttactga
     2641 tttctttttc gaaaatatca gctacttcct ggctccactt cttcgttttt ccctttttac
     2701 gaaactcagc gatctctatt tgctgagtgt tgccgcccag ttcttcctga atggttttta
     2761 tctgttgttg taagaaatat tctttctgtt gctggttgag gtcctcgcgg gttttcatct
     2821 ggatattcat cttcaactcc agtacttgtg tctcgcggtt aaggatcatc agcagcctgt
     2881 atgcttgttc cttttcgtca cttatactta acagctcctg tttttccctg ttttcaatgg
     2941 gcaggttggt ggcactgtag cttaccaaca taccggtgaa cgactcgtga ttgatggttt
     3001 gcagcagctc cttgggggga tcgccaagaa tgttgagcat atggttcata gcgtccctta
     3061 tggaatccag aatggcgctg aactccttgt cgtctttctc cgctcttttc gattccagga
     3121 tcctgtagtt ggccgcaaaa tagggctcgg tttgcgtgaa ctctgtccat tcgaatcttt
     3181 tctttgcctg gacaatgatg ctcaggttgt ttttgtcgag ctcaatcacg cgtattactt
     3241 cggcgatgac acctattgaa tatagatctt ccttctcagg ttcttcgacg ttgttgtctt
     3301 tttgacagac cagaccgata tagcggtgtt ttttgcccaa tgcccggact aacttgagtg
     3361 acttgctccg tcctacagag atggggagcc cgcttcccgg gaaaacgatg gtgttacgta
     3421 acggtaatat aggaagactt tccttcaggg aactttcatc gatctcctgg ctgccgtcca
     3481 ggaaatctcc ggcaataaac gatatgacgt tgctttcgct ctcttccggt tttaatacgg
     3541 ataatttatt ttgaaacata tttatttact tatcttcggt acggttcaat tcatatttta
     3601 tttcggaaaa acagaatatg tgttattttt gttgtcgcaa tgcctatatc ttacaccaaa
     3661 ttccgtgcca aatattgatt tttaccgttt atggggaaca actcttttaa atttaagcaa
     3721 tttactgttt ttcacgacag gtgtgccatg aaagtcggca ccgatggcgt cctgttgggt
     3781 gcgtgggcag attttatcga ttgccaatcg atcctggacg tgggaacggg ctcaggctta
     3841 attgccttaa tggctgcaca acgaaacagt ctggcaaaaa ttacagctat cgagatggat
     3901 gcagatgcgg ctttgcaggc cagggagaac gttgataatt ctcgttttaa agagcgcata
     3961 gaggtgttgc ctgtttcgtt ccgggatttt ttcccaacag tgacagggaa atttgactcg
     4021 attgtttcca accctccttt ttttgttaat tcgctgccat cgcccgatct gaaaagaacc
     4081 ggtgcccggc atatggattc gcttactccg gctgaacttt tcaaaatgag caggaagctt
     4141 cttaatgata cgggaaccct ttcgctgatc tatccgtttt cggaaaggga caatataatg
     4201 aacgtgtctg aaaaagaaaa tttccattta tcccgtgaaa cggttgttct tccgacccgg
     4261 tctgcttctc ccaagcgaat tttattggag tttactaccc gaagcgaaac ggagacggat
     4321 aggaacgaat tggttattga ggagaagcgt catgtttaca cacctgaatt tatccacctg
     4381 gtacaggatt tctacctcta tttatgaaat gaaattttta ttttttatct ttgccgaatg
     4441 acttataatc ccattttaat aaaaaaaatt ctggtattcc ccgtttttat gcttttgtcg
     4501 ctggggttta tgcaggcagg tgacaccctt cgtgtgaaac aaacgcaagt tcctatattg
     4561 atagaccgca ttgataatgc ccttttttat atcagtatcg atgcgaggga ggctgtaaaa
     4621 ttgaccgaat tgaaacttaa gttctccgaa agctcaaacc tgaacgaggt ggagtcttta
     4681 aaactctatt atggaggaac cgaagccgtt cagcgtaaag gacaaacatt ttatcatcct
     4741 gtacaatacc tgtcgggatt cacgccgggt aaaacccgtg aggcaaatcc ttcctattcg
     4801 gtgaaggttg ttgaacaaaa atcgccaggg atcgaagtgg ccttttcggc aaattacccc
     4861 cttttcccgg ggatcaacta tttctggatc agccttcaga tgaaacccac cacctccctg
     4921 ttgtcgaaaa ttgatgtgaa tctggtttca gcaaagctcg acggaagaga tgctcccata
     4981 tcgtttgcca ataatacgga aacacaccgg atgggagtgg gggttcgcca tgcaggagac
     5041 gatggcgttg ccgccttccg tattccaggc atggtcacca gcaatagagg aacgttgctg
     5101 ggtgtttacg acgtccggta caacaacagt gtggatttac aagaacacgt tgatataggg
     5161 ttgagccgga gtaccgacaa gggccagacc tgggagaaga tgcgtattcc ggttgcgttc
     5221 ggtgaatatg gcggattacc caaggcccag aatggagtgg gggacccatc cattttggtg
     5281 gacaggaaga cagggacaat ctggattgcc gccctgtgga cgcacggcat gggaaattac
     5341 agggcatggc acaactcgca agccggcatg gatgaaaaac ggacggggca gctgatattg
     5401 acaaaaagca tggatgacgg taagacctgg tcggaaccca tcaacatcac ttctcaggta
     5461 aaagaccctt cgtggcattt attgctgcag ggtcccggaa ggggaatcac catgcaggac
     5521 ggaacattgg ttttccccat tcagtttatt gactctaccc ggatacccaa cgccggcatc
     5581 atgtacagca aggacagcgg acagacctgg aaaattcata atcacgcccg tacaaacaca
     5641 acggaagcgc aagtggcgga agtcgagccc ggcgttttga tgcttaatat gcgcgataat
     5701 cgcggaggaa gccgtgccgt ttccataacg aaggatttag gcaaaacttg gacggagcac
     5761 ccgtcaaaca gaagcgtctt gcaggaaccg gtttgcatgg caagcctgat aaaagtggat
     5821 gccaatgaaa atgtactggg taaggatata ttgctgttct ccaatcccaa caccaccgaa
     5881 ggaagacatc acattaccat caaagccagc ctggacggag gattatcgtt tcccgaagaa
     5941 taccaggtgc tgttggatga agatcccggt tggggctact cttgcctgac tgtgattgat
     6001 aaagaaaccg ttggaatcct ttacgaaagc agcgtggcac acatgacttt ccaggcagtg
     6061 aagctaaggg atattattaa aggttccagg caatagatat tcaggctcat ttaacatcaa
     6121 caacatatga aaaaatactt gtttattttc agcttactat ccaccatggt tatggctcaa
     6181 gaaactccta tcctcctctt ccctgatggc gctcccggag aaacaacaaa aatgaagcaa
     6241 aaagacgatc tgtcgggcaa caaggtagcc ggatgtccgg tgctccgcat cagcgatgtg
     6301 agcgagccta cactgacgtt tttcccggct cctgcagata ataactcggg cgccaccatc
     6361 attgtgaatc cgggaggagg atacaatatt ttggcataca acctggaagg gacggaaatc
     6421 tgcaagcggt tcaacagcca cggagttaat tgtgtgctgg tgaaataccg cgtgccccga
     6481 cgcgaaggcc ttgagaagca tcaggcgccg ttgcaggacc tgcagcgagc cattgcctac
     6541 acacgttcgc acgcaggtga gtggaaaatc gatccaaacc ggataggcgt gatgggattc
     6601 tcagccggcg cccatttagc ggccatggcg agcacggcgt acgatgaacg cacctatccg
     6661 gccgtggatg cggcggacaa tgcgagcctc cgtcccgatt tttgtgtcct gatctatccg
     6721 gcatacctcg acggcgaaaa tttctccatc tcacccgaat tgaaagttac cgaaaagacg
     6781 ccgcccactt tcatcgttca aacccaggac gaccatcggt tgctcaacag cagcctcttc
     6841 tactactatg cgctcaaaga agcaaaagcc cctgttgcta tgagcttgta tccgtcagga
     6901 gggcacggtt atggactgcg gaacacgggc gatctggtaa atgaatggcc ttttcgtgtc
     6961 atgagttggc tgcacgatat aaaaatggtg gagtgattct tgccttaaaa gagacggtac
     7021 atgtttttat aattgaagag tttgatctct gcggtgtcgt tctcgaaatc tccggcatac
     7081 aagaccgctt tatccgtcaa cctgtctttt aataatgcat tcagcgaacg gattcctgtt
     7141 tcgaaatcgg gatggtaggt ttgggatgat ttgatttcaa cggctgaata atcgctcccg
     7201 tttttgaata gcaagtccac ctcaacgccg ttgctgtcgc ggtaaaagag caaattgctt
     7261 tctttacccc gattgtaacg gtttttaagt gcttccatca cgataaagtt ttcgaacagg
     7321 tggccacgca ttttgtccct tgtcagctgc tgttccgttt ctatgcctaa caaataacaa
     7381 gccaaaccgg tatcgcagaa gtagagtttc ggtgttttgg tcaaccgttt cctcatattt
     7441 tcatgaaaag gagttagtag cttgatgata taggatgcct gcaagacaga aagccaggat
     7501 ttgacggtat tggcggaaac acccacttcg cccgacagtt cggaggcatt gaacaagctg
     7561 cctatgcgcg cggcacacag ccgaaggaat gtttgaaact gcatcatgtc ttt
//
LOCUS       DAOSDR010000114         7563 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrimonas sp. isolate AMR_MDS_1826
            NODE_3572_length_7563_cov_3.637187, whole genome shotgun sequence.
ACCESSION   DAOSDR010000114 DAOSDR010000000
VERSION     DAOSDR010000114.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863355
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrimonas sp. (wastewater metagenome)
  ORGANISM  Petrimonas sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Dysgonomonadaceae; Petrimonas.
REFERENCE   1  (bases 1 to 7563)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7563)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:10:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,720
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 35
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7563
                     /organism="Petrimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3572_length_7563_cov_3.637187"
                     /isolate="AMR_MDS_1826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2023866"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(47..295)
                     /locus_tag="PLI30_05880"
     CDS             complement(47..295)
                     /locus_tag="PLI30_05880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI79120.1"
                     /translation="MGFLIKVLLVFAVIYMVINFLGRALFGTRRKPQANPHSRHSQQQ
                     QNEPETQEARILDYQKKSFETTDAIDVEFEEIKQKERR"
     gene            complement(371..1081)
                     /gene="pssA"
                     /locus_tag="PLI30_05885"
     CDS             complement(371..1081)
                     /gene="pssA"
                     /locus_tag="PLI30_05885"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009859953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003882 - CDP-diacylglycerol-serine
                     O-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HOI79121.1"
                     /translation="MKKQIPNIITLLNLFSGCIAMVMAFKGNFSAVVMWVILAAAFDF
                     FDGWTARSLGVSSKIGVELDSLADIVSFGVAPASAVFMLLEEFTLFPSFLAQPEPIIP
                     YLAFLIPVFSAYRLAKFNIDDRQTTSFLGLPTPANGLFWISYVYGTHQAAVTNGFYFY
                     LTVVFILVLSILMVSEIPMFSLKIKSLKIRGNERQLLLVVVIVAFVLLWGITGVAWGI
                     LVYIVMSIATMRKNKLIR"
     gene            complement(1062..1754)
                     /locus_tag="PLI30_05890"
     CDS             complement(1062..1754)
                     /locus_tag="PLI30_05890"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009347456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase family protein"
                     /protein_id="HOI79122.1"
                     /translation="MLIHKEGRKTLFVTTVILVLLNGFMFRFFPESPFSFILLFISVV
                     VFALMMNFFKKPSRVYEGDLLDYVNAPTDGKIVVIEKIFEKKYFNEERIQISIFMSFF
                     NAHSNWIPVTGKVVHYSHEKGNFHAAYLPKSSHENERSNIIIETPDGHRVLTRQIAGA
                     VARRIVTYVREGEYYHIGDRLGFIKLGSRMDVFLPIDSEILVQIGDEVRANETLLARL
                     PQRLKGDEKTNS"
     gene            complement(1767..>2148)
                     /locus_tag="PLI30_05895"
     CDS             complement(1767..>2148)
                     /locus_tag="PLI30_05895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HOI79123.1"
                     /translation="VSLIGFLLQKVFESVNLNFINRILGGVVSMGTTVVVLSILLNLI
                     LMLDGEEKIIKPNIKQKSFFYEKVRVAVPAIVPYLNKEVWEEFVPEKYRKQIENSGSI
                     NYDTENGQPIDSAFQKKYFETDSI"
     assembly_gap    2149..2158
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2159..2400)
                     /locus_tag="PLI30_05900"
     CDS             complement(<2159..2400)
                     /locus_tag="PLI30_05900"
                     /inference="COORDINATES: protein motif:HMM:NF014704.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009403 - toxin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HOI79124.1"
                     /translation="MLNGFDLIILIFLLVAFVNGYRKGLVMMLVGLATVILAAVFAGK
                     LAVTVLPYLQKTFDFSPQVTHVLSYVAAFLAIAAVVS"
     gene            complement(2404..3246)
                     /gene="murI"
                     /locus_tag="PLI30_05905"
     CDS             complement(2404..3246)
                     /gene="murI"
                     /locus_tag="PLI30_05905"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007654946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HOI79125.1"
                     /translation="MIAGTEQQQSPIGIFDSGYGGLTVLSEIKSLLPQYDYMYLGDNA
                     RAPYGNRSFEIVYQFTKDAVKWFFGQNCHLVILACNTASAKALRTIQQVDLPAMDPSR
                     RVLGVIRPTVESVAGFTKTGHIGVFGTSGTINSQSYDIEITKLHPHLKVTGEACPIWV
                     PLVENNEFDKPGADYFVKQHIHNLLKKDDKIDTIILGCTHYPLLITKIKQFLPQGIQV
                     ISQGKYVAESLKEYLQRHPEMEKRCSKDGTVRYFTTDLPGKFADMASLFLKENIEAQK
                     INID"
     gene            complement(3267..3788)
                     /locus_tag="PLI30_05910"
     CDS             complement(3267..3788)
                     /locus_tag="PLI30_05910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006798800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HOI79126.1"
                     /translation="MNKTKLISLGVLFLILSATLSAQNPQVAVAYVNTTDLLSAFPAK
                     EEATQKLVALSENYKKELELMQNEYNKKYSDYITYQGSLAENIKLRRMQELTELENKM
                     QQFMQLAQKDIEQQEKAMLEPLKKQISDAIRQVGVEHNFTVIYDLANPGIAFVNPVAV
                     DANPLVKAKLGIN"
     gene            complement(3936..6914)
                     /gene="infB"
                     /locus_tag="PLI30_05915"
     CDS             complement(3936..6914)
                     /gene="infB"
                     /locus_tag="PLI30_05915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009275402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HOI79127.1"
                     /translation="MAERLSKVARNLNVGINTLVEFLHKKGITVEANPNTKIEEEQYN
                     LLVAEFSKDKKIKKEAIENLERMHRKDDKRETIAIEGYEIEEKPKKKIEKETIKAEIP
                     DDLKPKFNVVGSIDLDEHKKLKKETEAKQEEEVEVEVEVEVEVEEVKPEEPVQKVEEV
                     ETVTPKPVEPKEIEVPHDEAPVVTEAPSSEAEETPEEVVNETANKEREDYSPQEQETE
                     AEEDITLHEKEKGPDEIFRIRKELKEPNIVVKGSIDLESINDRTRPPRKSRAQRKKER
                     LEREKEQNAKKAKEEAVKSLKKESIEEKEKKKGAVINTAEEDSKKKKRKRIDKGKVNI
                     EKAGAHGESTRRDKKVLRKPIKAEVSEEDVQKQIKETLARLTEKRGGKGGARYRREKR
                     EAVSQRQREEIKQQERESKILQLTEFVTANDLANMMDVPVTSVIATCMNLGVMVAINQ
                     RLDAETINIVADEFGYKTKFVSADVIEAIAEEEDNLEDLLPRPPIVTVMGHVDHGKTS
                     LLDSIRNTNVIEGEAGGITQHIGAYNVKLPDGRKITFLDTPGHEAFTAMRARGAKVTD
                     VAIIIVAADDNVMPQTVEAINHAGAAGVPIVFAINKVDKPGANPEKIKERLAEMNYLV
                     EDWGGKYQSQDISAKKGTGINELLEKVLLEADLLELKANPNRKASGSIVESSLDKGRG
                     YVATVLVENGTLKQGDVLLAGSYYGRVKAMFNERNQKINEAGPSEPALVLGLNGAPQA
                     GDTFNVMESDQDARNVAARREQLQREQSLRTQKMLTLDDIGRRIAIGNFQQLNIIVKG
                     DVDGSVEALSDSLIRLSTEEIQVNVIHKAVGQISESDVTLAAASDGVIIGFQVRPSLG
                     ARREAEKQGIDIRLYSIIYDAIEEVKAAMEGMLSPDIKEEITANVEVLDVFKITKVGT
                     VAGSMVRDGKIKRTHKIRLIRDGIVIFTGELDSLKRFKEDVREVASGYECGISIKNFN
                     DIKVGDIIESYEQIEVKKTL"
     gene            complement(6969..>7563)
                     /locus_tag="PLI30_05920"
     CDS             complement(6969..>7563)
                     /locus_tag="PLI30_05920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010536141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HOI79128.1"
                     /translation="KGIARIPGERAKVAVEAYDDRIDPVGACVGMKGFRIHGIVRELR
                     NENIDVINYTNNTNLFIQRALSPAKINSISVKEEERRAEVFLYPDEVSLAIGKGGANI
                     KLASMLTGYNIEVFREIDDFDEEDIYLDEFRDEIDGWVIDQLKRIGCSTAKNVLAMPR
                     ERLIKEADLEENTVDEVLKILRYEFEDEDTTDEEPEI"
BASE COUNT         1765 a         1764 c         1628 g         2396 t
ORIGIN      
        1 cctgatattt atcaaacggc tgtactctat ataacaagaa tggatgttac ctccgctctt
       61 tctgtttgat ctcttcaaac tccacatcaa tggcatcggt agtttcaaaa ctttttttct
      121 gataatcgag gatccgtgct tcctgtgttt ccggttcatt ctgttgttgt tgcgaatgac
      181 ggctgtgggg gttggcttga ggttttcgtc tggttccgaa aagggcacgc cccaaaaaat
      241 tgattaccat gtaaataact gcaaatacca gtaagacctt aattaaaaat cccatgttct
      301 agtcatttaa ttcaaaaata tgtgtaaaga taagaggaat aattaaatgt atttcaacgc
      361 ttttaaaaaa ttaacgaatg agtttgtttt ttctcattgt ggcaattgac ataacaatgt
      421 acaccagaat tccccaagct acccccgtaa tcccccaaag taaaacaaac gcaactatca
      481 caacaacgag caatagctgt ctctcatttc ctctgatctt caagcttttt attttgagtg
      541 aaaacatggg gatctcggaa accatcagta ttgagagaac cagaataaaa actacagtca
      601 ggtagaaata aaatccgttg gtgacagccg cctgatgcgt accgtacacg tagctgatcc
      661 aaaataatcc gttggcgggt gtaggaaggc cgagaaatga agttgtctgg cggtcgtcga
      721 tgttgaattt tgccagcctg taggccgaga aaaccgggat caggaaagcc aggtagggga
      781 tgatcggttc cggttgggca agaaaagagg ggaaaagtgt aaattcctcc aacagcataa
      841 agacggcaga cgcaggtgca accccgaaac tgactatgtc ggccaaggag tccaattcta
      901 ctccgatctt ggaagagacc cccagtgacc gtgcggtcca tccgtcgaaa aaatcgaagg
      961 cggctgccag tatgacccac atgacgaccg ctgagaaatt tcccttgaat gccatgacca
     1021 tggcaataca acctgaaaac aggttcagca aggtaattat attaggaatt tgttttttca
     1081 tcccctttta gtctttgcgg taaccgtgcc agcagtgttt cgttggctct gacctcatcg
     1141 cctatttgaa ccaggatctc gctgtcgatg ggcagaaaga catccatcct ggagcccagt
     1201 ttgataaaac ccaggcggtc tccgatatga taatattcgc cttccctgac gtaggtgaca
     1261 attcgacgcg ccaccgcacc ggcgatttgc cgggtaagta cacggtgccc gtcgggtgtc
     1321 tcaatgatga tgttgcttct ctcgttctcg tgacttgatt taggcaagta tgctgcgtgg
     1381 aaattacctt tttcgtgtga ataatgcact acttttcccg tgacgggtat ccagtttgag
     1441 tgtgcattaa aaaatgacat aaagatggaa atttgtatcc gctcttcgtt aaagtatttt
     1501 ttttcaaaaa tcttttcaat cactacgatt ttcccgtcgg taggtgcatt cacataatcc
     1561 agcaagtccc cttcgtaaac gcggcttggc tttttgaaga aattcatcat cagggcaaaa
     1621 accactactg aaataaacaa caggataaaa gaaaagggac tttccgggaa aaaacggaac
     1681 ataaatccgt ttaataatac caaaataacg gttgtgacaa acagcgtttt tctcccttcc
     1741 ttgtgaatta gcatgatgta aggtttttaa atagaatcgg tttcgaaata ttttttctga
     1801 aaagcggaat caatgggttg tccgttttcc gtatcgtagt ttatgctgcc tgagttctcg
     1861 atttgttttc tgtatttctc cggaacaaat tcttcccaca cttccttatt caggtaggga
     1921 acgatggcag gtactgcaac acggaccttt tcgtaaaaaa aggatttctg ttttatattg
     1981 ggtttaatga ttttttcttc tccgtcgagc ataagaataa gattgagcag aatacttaga
     2041 acaacaacgg ttgtccccat ggaaaccaca cctccgagaa tccggttgat aaagttgagg
     2101 tttaccgatt cgaacacttt ttgtaagaga aaaccgatta atgagacann nnnnnnnnga
     2161 gacaacagcc gctatggcta ggaaagcagc cacgtaggag agtacatggg taacctgtgg
     2221 agagaaatcg aacgtttttt ggaggtaagg cagaacggta acagccagct tgccggcgaa
     2281 aacggcagcg agaatgacgg ttgccaaccc aacgagcatc ataaccagcc ctttcctata
     2341 tccgtttacg aatgcgacaa gcagaaaaat aagtatgatg agatcaaacc cgtttaacat
     2401 aattcaatcg atgtttattt tttgagcttc aatgttctct ttcagaaata aggacgccat
     2461 atctgcaaat ttcccgggta ggtcggtcgt gaaatatcta actgtgccgt ctttggagca
     2521 tcttttctcc atttccgggt gacgttgaag gtactctttc aggctttccg ccacgtattt
     2581 cccttgcgat ataacttgaa taccctgggg taaaaactgt tttattttag tgatcaagag
     2641 tgggtaatgt gtgcaaccca ggataatggt gtcgatttta tcatcctttt tgagcaggtt
     2701 gtggatgtgt tgttttacaa aataatcggc tccgggttta tcaaattcat tgttttccac
     2761 cagtgggacc catatgggac aggcttcacc cgtcactttc aggtggggat gcagtttggt
     2821 aatttcgata tcgtacgatt gggaatttat ggtgcctgat gtcccgaaca caccgatatg
     2881 gcctgtcttt gtgaaaccgg caacggattc gaccgtaggg cgaatgacgc ccagcaccct
     2941 tctcgatgga tccattgcgg gtaagtcgac ctgttggatg gtccgcaacg cttttgccga
     3001 tgcagtgttg caggctaaaa taaccaggtg gcagttctgc ccgaagaacc attttacggc
     3061 atcttttgta aattgataca cgatctcgaa ggagcggttc ccatagggtg cccgggcatt
     3121 atcacccaga tacatgtaat cgtattgagg aagtaacgat ttgatttcgg acaatacggt
     3181 aagcccgcca tacccggaat caaaaatacc gataggcgat tgttgttgtt cagtacccgc
     3241 tatcacactt tttggcgata ataagtttag ttgattccca gttttgcctt taccaaagga
     3301 ttggcatcca ctgctaccgg gttcacaaaa gcaattcccg gattggcaag gtcgtagata
     3361 accgtgaaat tatgttcgac acctacctga cggatggcat cggaaatttg tttctttaac
     3421 ggttcaagca tagctttttc ctgctgctcg atgtcttttt gagccaattg cataaactgt
     3481 tgcatcttgt tttccagttc agtcaactcc tgcatcctgc gtaatttgat gttttcggcc
     3541 aatgaaccct gataggtgat gtaatctgaa tattttttgt tgtattcgtt ttgcatcagc
     3601 tcgagttctt ttttatagtt ttcactcaat gctacgagtt tttgtgtggc ttcttccttt
     3661 gccgggaaag cactcagaag atcggttgta ttcacataag caaccgctac ctgtggattt
     3721 tgggccgaaa gggtggcgct cagtattaaa aatagaacac ccaagcttat caactttgtt
     3781 ttgttcataa taatcctatt ttagatgtaa ataaattttg ttttataccg taacctgagt
     3841 tggcaattgt aatttcctcc ttgtgtgttt tagccgcacc ccgcttccgg cgggatgcgg
     3901 ctaagtaaag gaattaaaat tacaatgtca actggttata aagtcttttt tacttctatc
     3961 tgttcgtaag attcaatgat gtcgcccact tttatgtcgt tgaagttttt gatactgata
     4021 ccgcattcgt acccggacgc aacttccctg acatcttctt tgaatcgttt cagtgaatcc
     4081 aattcgccgg taaagataac aattccgtcc cgaataagac gaattttatg ggtacgtttt
     4141 atttttccgt cccgcaccat tgaaccggct acagtaccta ctttagtgat tttaaagaca
     4201 tcaagcacct ctacgtttgc tgtaatttct tctttaatat cgggggaaag cataccttcc
     4261 atggcggcct tgacctcttc gattgcgtcg tagataatgg agtacaaacg gatatcgata
     4321 ccttgtttct ccgcttcacg ccttgctccc aaagaaggac ggacctggaa tccaataata
     4381 actccgtctg atgctgccgc gagggtaacg tccgattcag agatttggcc tacagctttg
     4441 tgaatcacgt taacctgaat ttcttcggta gacaagcgga tgagcgaatc tgaaagtgct
     4501 tctaccgaac cgtccacatc accttttaca atgatgttca gctgctggaa gtttccgata
     4561 gctatcctgc ggccgatgtc atccagggta agcattttct gcgtacgcag cgattgttcg
     4621 cgctgtaact gttcacggcg tgcggcaacg ttgcgcgcat cctggtcgct ctccatgacg
     4681 ttgaacgtat ctccggcctg cggtgcgccg ttcagcccga gcaccagcgc cggctctgac
     4741 ggccctgctt cgttgatttt ctggttccgc tcgttgaaca tcgcttttac gcgtccgtag
     4801 tatgatccgg ccaataacac atcgccttgc ttgagcgttc cgttttcaac caagacagtt
     4861 gccacgtagc cccggccttt gtcgagtgac gattccacaa tggagcccga tgctttccgg
     4921 ttggggtttg ctttaagttc caacaagtct gcttcgagca gcactttttc gagcaattcg
     4981 ttgatgcctg ttcctttttt ggcagagatg tcttgtgact ggtattttcc gccccaatct
     5041 tctaccaggt aattcatctc agccagtcgc tcttttattt tttcaggatt tgctcccggc
     5101 ttatccactt tattgatggc aaaaacaatg gggacacctg cggccccggc gtggttaatg
     5161 gcctcaacgg tttggggcat cacattgtcg tcggcagcta caatgatgat agctacgtcc
     5221 gttactttag ccccgcgggc acgcatagcg gtaaatgcct cgtggccggg agtatccagg
     5281 aacgtgattt ttctgccgtc aggcagtttc acgttgtacg cgccaatatg ctgggtgatt
     5341 cctccggctt ccccttcaat aacgttggtg ttgcggatgc tgtcgagcag ggatgtcttg
     5401 ccgtggtcaa catgtcccat aacggtaacg attggcgggc gtggtagcaa atcttcgaga
     5461 ttatcctctt cttcagctat ggcttcgata acgtcagcgc tcacgaattt ggttttgtag
     5521 ccgaattcat cggcaacaat attgattgtt tctgcatcca ggcgttggtt gatagccacc
     5581 ataacgccca ggttcatgca ggtagcgata acgctggtaa cggggacatc catcatgttt
     5641 gccaggtcat ttgccgtaac aaactcggta agctgcaata tcttgctttc gcgttcctgt
     5701 tgtttgattt cttcgcgttg tcgctgagaa acggcctccc gtttttcgcg gcgatacctt
     5761 gctccgcctt ttcccccacg tttttcggtc aaccgggcaa gtgtttcctt tatttgtttt
     5821 tgaacgtctt cttcgcttac ttcggctttt atcggttttc ggagaacttt tttatcccga
     5881 cgggtgcttt ctccgtgagc tccggctttt tctatattga ctttgccttt atctatgcgt
     5941 ttgcgttttt tctttttact gtcttcctcc gctgtattga ttaccgcccc tttcttcttt
     6001 tctttttctt cgatcgattc ttttttcagg ctttttacgg cttcttcttt cgctttcttg
     6061 gcattttgct ctttttcacg ctccaggcgt tctttcttgc gttgtgccct tgatttccgg
     6121 ggtggacggg tcctgtcgtt tatagattcc agatcgatgg agcctttcac cacgatattg
     6181 ggttctttca gttctttacg tatccgaaat atttcgtccg ggcccttctc cttctcgtgc
     6241 agagttatat cttcttctgc ttctgtctct tgttcctggg gggagtagtc ttcccgttcc
     6301 ttatttgccg tttcattcac cacttcttcc ggggtttctt cggcttcaga tgaaggggcc
     6361 tccgttacaa caggtgcttc atcgtggggg acttcaattt ccttcggctc caccggtttc
     6421 ggtgtaactg tttcaacctc ttcaactttt tgtacaggct cttccggttt tacttcttca
     6481 acctcaactt caacttcaac ttcaacctca acttcttctt cttgtttagc ttcggtttct
     6541 tttttcagtt tcttgtgctc gtctaaatcg atgcttccta cgacgttgaa ttttggtttc
     6601 aagtcatccg ggatttcagc ttttatggtc tctttttcta ttttcttctt gggtttttcc
     6661 tctatttcgt acccttcgat ggcaattgtt tcccgtttat catccttccg gtgcatcctt
     6721 tcgagatttt caatggcctc ttttttaatt ttcttgtctt tgctgaattc ggccaccaac
     6781 aaattatact gttcttcctc aatctttgta ttaggatttg cttcaacagt aattcctttc
     6841 ttgtgcaaaa attcaaccaa cgtgttgatc cctacattta aattcctagc aactttactt
     6901 aatctttcag ccatatagtt gagtttgtcc tttcttaatt tattggttct gtgaacagtt
     6961 taacagtctt aaatctccgg ctcttcgtcg gtagtatctt catcttcaaa ttcgtaacga
     7021 agaatcttca atacctcgtc cacggtgttc tcttccaggt cggcttcctt gatgagcctt
     7081 tcacgcggca ttgccaacac gtttttggcc gttgagcaac cgattcgttt gagctgatcg
     7141 attacccatc cgtcaatttc atcgcggaat tcgtccaggt agatatcctc ttcgtcgaaa
     7201 tcatcgattt ccctgaaaac ttctatgttg tatcctgtca gcatagatgc cagtttaata
     7261 tttgccccgc ctttaccgat tgccagtgaa acttcatcgg gatagagaaa tacctcggca
     7321 cggcgttctt cttccttaac ggagatagag ttgatctttg cagggctaag tgctcgctga
     7381 ataaagagat tggtgttgtt cgtatagttg ataacatcaa tattctcgtt gcgaagctca
     7441 cgtacaattc cgtgaatacg gaaccctttc atccccacac aggctccaac cggatcaatg
     7501 cggtcgtcgt aggcctcaac agccactttg gcccgttctc ccgggatacg ggcaatacct
     7561 ttg
//
LOCUS       DAOSDR010000115         7559 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrimonas sp. isolate AMR_MDS_1826
            NODE_3574_length_7559_cov_4.291978, whole genome shotgun sequence.
ACCESSION   DAOSDR010000115 DAOSDR010000000
VERSION     DAOSDR010000115.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863355
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrimonas sp. (wastewater metagenome)
  ORGANISM  Petrimonas sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Dysgonomonadaceae; Petrimonas.
REFERENCE   1  (bases 1 to 7559)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7559)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:10:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,720
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 35
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7559
                     /organism="Petrimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3574_length_7559_cov_4.291978"
                     /isolate="AMR_MDS_1826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2023866"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..166
                     /locus_tag="PLI30_05925"
     CDS             <1..166
                     /locus_tag="PLI30_05925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014709557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HOI79129.1"
                     /translation="EVVSNALTGFNHSKYPWLEWDRASMSGKFLHLPERADIPENIKE
                     QLIVELYSKQ"
     gene            171..1172
                     /locus_tag="PLI30_05930"
     CDS             171..1172
                     /locus_tag="PLI30_05930"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018109216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HOI79130.1"
                     /translation="MAILAFQKPDKVIMIQENAFSGKFEFRPLEPGYGITIGNALRRI
                     LLSSLEGYAITSVKFDGVEHEFASIPGIIEDVTTIILNLKKIRFKRIVEEFDTEKVSL
                     NVSGTNVFKAGDVGKLLTGFEVLNPELEICHLEKKAELRLELTINKGRGYVPAEENRA
                     MLPSEDVQTIAIDSIFTPIRNVKYEIENYRIEQKTDYEKLIIEVTTDGSIQPKDALKE
                     AAKILIQHFALFSDEAKMMVETTGTENIEEFDEEVLHMRQLLKRKLSDLNLSVRALNC
                     LKAAEVETLGHLVMHNKNDLLKFRNFGKKSLTELEELLDNLSLSFGMDISKYKLDKD"
     gene            1178..1594
                     /gene="rplQ"
                     /locus_tag="PLI30_05935"
                     /pseudo
     CDS             1178..1594
                     /gene="rplQ"
                     /locus_tag="PLI30_05935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873882.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005762 - mitochondrial large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
     gene            2032..3270
                     /locus_tag="PLI30_05940"
     CDS             2032..3270
                     /locus_tag="PLI30_05940"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019541312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HOI79131.1"
                     /translation="MKKVVVIGCGFGGLQFVRHLKKNRFDILVIDKINHHQFPPLFYQ
                     VAASQIEPSTVSFPIRKIFQNRKDVRIRLAKVYSLNAGEKTIDTSIGTFSYDYLVIAT
                     GSRTNFYGNRQVEEYALGLKSTYQAINVRNFILMNFEKLLYETDKEGLYNIVIVGAGA
                     TGVEMAGAFVEMKREVLPKDYPHIDSSKVSVYLLEGGSNTLSTMSRFAQDYSEKYLRD
                     MGVIVKTNVIVTNYDGNEITLNNGETIRSKSVIWSAGITGNALDGIPPQSIVRGGRIK
                     VDRINRVEGLSDVFAIGDVAYMETSNYPKGHPQVANVAIGHGKNLAKNLQYIHLRKTN
                     LRPYEYRNLGTMATVSRNKAVVDLPFAKFKGRFAWLVWMFLHLMLILSVRNKLIVFIN
                     WAWNYVTRNNSLRLILKDQD"
     gene            3428..4069
                     /locus_tag="PLI30_05945"
     CDS             3428..4069
                     /locus_tag="PLI30_05945"
                     /inference="COORDINATES: protein motif:HMM:NF039786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6448 family protein"
                     /protein_id="HOI79132.1"
                     /translation="MKTKELNVEVVDVKNVPARRNSFKSVLPGLFISVLLFFASTPLF
                     AHCDSYDGPVIKDAVKALETNNVNLVFKWITREQEAEISGLFNKVYGLKSGDREIYAI
                     LEKHFFETLVRLHRETEGAPYTGLKPAGTTKQIIVLSDKAIENNDIHKLLVQLNNHIG
                     SVIREKYHKVETLNKGKNNSAEEGRAYVEAYVDYTHSVEALHDILENGSGHNH"
     gene            4163..6697
                     /locus_tag="PLI30_05950"
     CDS             4163..6697
                     /locus_tag="PLI30_05950"
                     /inference="COORDINATES: protein motif:HMM:NF039587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5916 domain-containing protein"
                     /protein_id="HOI79133.1"
                     /translation="MMKIYYTLLFFLFSLLAFSQTRTYTTSRTQQVPDIDGQLTDDCW
                     LNAVWGSDFVQFEPHSGAQPSQQTQFAIVYDDDNLYIAIKALDAEPGKIVRRVSRRDD
                     ADGDAVAIAIDSYEDKMTAFAFLVTAAGSKTDWRLTEENDEDYSWDPIWEVRTSLQPD
                     GWYAEIRIPYSQLRFGKNNQYRWGLEVMRHVYRHQERSFWQPIDKASAKFIGNFGVLD
                     GIRNINPKKDIEIVPFALSQLTLSEKEKGNPFATGTRTKASVGVDGKVSITHDITLNF
                     TVNPDFGQVEADPSVVNLTNFETYFDEKRPFFVEGSNIYHYPFDLTNNERNKLFYSRR
                     LGREPHLSYTKKEGEYVREPGRTNILGAVKVSGKTREGTSIGILNAVTQKMYSEIDHS
                     GERSKYAVEPMSNYFVGRLEQDLDSGNLIVGGIFTATNRKIDEDQFRTLPGSAYTTGL
                     NLTRYWKEKSYFITARVFFSHLRGTQEAMIQLQKSSARYCQRPDAKHLELDGERTSLS
                     GFGGSVSGGKASGHFNLLGFSSWSSPGLEFNDVGFKPNADMFLNGIWAAWNEWRPKGI
                     VNEYQFNGSWYNVRDFSGTSLEWGNEAFGYIQFTNYYDIAGGLNINYPGLSNSMLRGG
                     PALKTPGVINSWFSVESDSRKKLSLEYEMNFSHGFEHSRRSISYGLDITYKPNNRLSL
                     SLYPQYVKATGHQQYVRTVTGDSPAYILSSIDQNIASMSVRVNYGITPDMSLQWYALP
                     YLFSGDYYGFKTVTHPRADKSSDRFTAMDTYPYDNPDFYFLQFRSNLVYRWEYKPGSV
                     LYLVWSQGRTVQGNEGVFRFRDYLGDLFEAAPQNDFLVKVSYAWIF"
     gene            6775..7551
                     /locus_tag="PLI30_05955"
     CDS             6775..7551
                     /locus_tag="PLI30_05955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005682591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5040 domain-containing protein"
                     /protein_id="HOI79134.1"
                     /translation="MKRIPVFTVLLALCILTLSAQDNASKKSYTFLLTGASFASPNNG
                     WFEIGCELSDANPLNRAIGGEAIADAANRIIDGTLYTIEELEHIDALVIMQVHDRDVF
                     DESQLKPNYTEYPVPFDRSNYAAAFDYVIKRYLADCYNLKFDRKSKYFNSRSGKPAVV
                     VLCTDWHDGRVTYNTSVRKLAEKWGFPVVEFDKFIGFSRNALHPVTGEQISRLFTGDK
                     QEIDGEIFGWHPENGKEQYIQQRMGAVFADTMRKIFPVKP"
BASE COUNT         2265 a         1538 c         1748 g         2008 t
ORIGIN      
        1 ggaagtggtt tcaaatgcac tcaccggttt caatcacagt aaatatcctt ggttagagtg
       61 ggacagagca tctatgagcg gtaagtttct gcatttgccc gaaagagctg atatccccga
      121 aaacattaaa gaacaattga tcgtagaatt atattctaaa caataatttc atggcaatat
      181 tagcattcca aaaacccgat aaagtaatca tgatccagga gaatgcgttc tccggaaagt
      241 tcgaattccg tccgctggag cccggatacg gcattaccat tggtaacgcg ttgcgccgta
      301 ttttgctttc ttctctcgaa ggatatgcaa ttacatcggt aaagtttgac ggtgttgaac
      361 atgaatttgc ttctattccg ggtataattg aagatgtgac gactattatc ctgaatctga
      421 aaaaaattag gtttaaaagg atagtagaag aatttgatac ggaaaaagta agtctcaatg
      481 tatcgggcac gaatgtgttt aaagccggag atgttggcaa attactgact ggttttgaag
      541 ttctgaatcc tgaacttgaa atctgtcatt tagagaagaa agctgaatta cggctcgaac
      601 tgaccatcaa caagggacgt ggatatgttc cagctgagga aaatcgtgct atgttgcctt
      661 ctgaagacgt gcaaaccatt gctatcgact ctattttcac gcctatcaga aatgtaaaat
      721 acgaaataga gaattaccgt atcgagcaaa agaccgacta cgaaaaatta attatcgaag
      781 ttaccacgga tggttccatc caaccgaaag atgcgttaaa ggaggctgct aaaatcctta
      841 tccagcattt tgcgttgttc tctgacgaag ccaagatgat ggtagaaacc accggtacag
      901 agaatatcga ggaattcgac gaagaagtgt tgcacatgcg tcaattgctg aaacgcaaat
      961 tgtctgacct gaatctttcg gttcgtgcgc tcaactgcct gaaagcggct gaagtggaaa
     1021 ccctgggtca tctggtgatg cacaacaaaa acgatttgtt gaagttcaga aacttcggaa
     1081 aaaaatcgct tactgagctg gaggagttac ttgataattt aagcctgtcg ttcggtatgg
     1141 atatctcgaa atataaatta gataaagatt aacgataatg agacacaata agaaaaataa
     1201 tcatttagga cgtaaagctg cacaccgcga tgccatgtta tcgaacatgg caacatcgct
     1261 catcatgcac aaacgtattt tcactactgt gcctaaagcc aaggaattaa gaaagtttgt
     1321 agaacctatc atcaccaaat caaaagagga caccaccagt tcgcgaagag tagttttcag
     1381 tattttgcaa aataaatttg cagttaccga actttttcag tatatttcgc aaaaagtagc
     1441 tgatcgtccc ggagggtata cccgcatcat caaaacaggc tatcgcttgg gagataatgc
     1501 agcaatgtgc ttcatcgagt tggttgacta caacgaaaac atgttgggcg acggcggagc
     1561 taagaaagca aaaacacgcc gttcacgtag aaaagctacg actgctgccg ctccagctac
     1621 tgccaccgat tctaaagcta aggaaccgaa agtgaaaaaa gaagaagcaa aagctgaaga
     1681 agttaaggct gaagaaaccg ttgaactaaa agatacaaaa gaagtgaatg cttcagaagt
     1741 tcctgtgaca gaaaaagttg atacacagga caaaacgcaa atcgctgagg gagcatctgc
     1801 cgaaggtgaa ggactaaaag actgaaccaa gatgtaacca cagcgaagct gtgattttta
     1861 aaatgttaaa cattaagaaa gcgccgcgag tgattcgtag cgctttctgc tttttacata
     1921 tttgtcaagc tgtccgaccc tttgaaaaca ttctgccgtt tcagacgttt tatgattaaa
     1981 atgaatgcgc atgatctatt aaaacttgat ttatcaacta atttttgaac aatgaaaaag
     2041 gtcgtagtta ttggttgtgg ttttggggga ttacagtttg tacggcattt gaaaaaaaac
     2101 agatttgata ttcttgtcat agataaaata aatcatcacc aatttcctcc tctgttttat
     2161 caggtagctg cttcgcaaat agaaccgtca accgtttcat tccccattcg taaaatattt
     2221 cagaacagaa aagatgtccg gatccgcctt gcaaaagtat attcccttaa cgccggagag
     2281 aaaacgatag acacttctat cggtacattt tcatacgatt atctggttat tgccaccggg
     2341 tcgcggacta acttctacgg gaaccgccag gtagaagaat atgcattggg attaaaatct
     2401 acttaccagg ccattaatgt acgaaacttt atcctgatga attttgaaaa actcctttac
     2461 gaaactgata aggaaggctt gtacaacatc gttattgtgg gtgcaggcgc cacaggtgtt
     2521 gagatggccg gcgcatttgt cgagatgaag cgcgaagtac ttccgaaaga ctatccccat
     2581 atcgattcgt ctaaggtcag tgtgtatctc ctggaaggag ggtcgaacac gctgtccacc
     2641 atgagtcgat ttgcacaaga ctattccgaa aaatacctgc gtgatatggg tgtgattgta
     2701 aaaaccaacg tgattgtaac caattacgac ggaaatgaaa tcacactcaa caatggcgaa
     2761 accattcgtt ccaaaagcgt tatctggtca gccggtatca ccggtaatgc acttgatggt
     2821 attcctccac aatccatagt gagaggcgga agaattaaag tcgaccggat aaaccgggtg
     2881 gagggactca gcgatgtttt tgctatcgga gacgtggctt atatggaaac ctccaactat
     2941 cccaaggggc atccccaggt ggcaaatgtt gccatagggc acggcaaaaa cttggcaaaa
     3001 aaccttcagt acatccattt aaggaagact aatctccgtc cttatgaata ccgtaacctt
     3061 ggaactatgg caactgttag cagaaacaag gctgtagtag acctgccctt tgctaaattt
     3121 aaaggacggt tcgcgtggct tgtctggatg ttccttcacc tgatgcttat acttagtgtg
     3181 cgcaacaaac tcattgtttt tatcaattgg gcgtggaatt atgttactcg aaataactcg
     3241 cttcggctta tcctgaagga tcaggattga tttgaccgtt tgaaaacgct tggtatgatt
     3301 caaccggaaa accatttttt tgccggaaat aggtcctaaa ttacaaagta ggtcgcacag
     3361 agggagagag atgtatactt ttgttttcag aaaattttat tattaacttt tttaaaatga
     3421 atttaaaatg aaaacaaaag aattaaatgt tgaagtagta gatgtgaaaa atgtacctgc
     3481 tcgtagaaac tctttcaaaa gtgtgttgcc tgggttgttt atttccgtac tgctgttttt
     3541 tgcatcaaca cctctctttg ctcattgtga ctcgtacgac ggccctgtga ttaaggatgc
     3601 ggttaaagcg cttgaaacaa acaacgtgaa tttggtcttc aaatggatta cccgggaaca
     3661 ggaagctgaa atatccggtt tgttcaataa ggtctacggt ttgaaatcgg gtgaccggga
     3721 aatttacgcc atccttgaaa agcatttttt cgaaacgctt gtgaggttgc atcgggaaac
     3781 ggaaggcgca ccctataccg ggttaaaacc ggcaggaact accaaacaaa taattgtgtt
     3841 gagtgataaa gccatcgaga acaacgatat tcacaaatta cttgtccaat tgaacaacca
     3901 tatcggaagt gtgatcaggg agaaatacca caaggtggaa acactcaaca aggggaaaaa
     3961 caattccgcc gaagaaggac gtgcttacgt ggaggcttac gttgattaca cgcattcggt
     4021 tgaagcgctt cacgacattt tggaaaacgg tagcggccat aatcattgag acggaggaga
     4081 gaaaggttga aggggtaaaa acgtcacaaa atttgtaacg gaaaaggcct ttgttacgtc
     4141 ttatggatat acccaacaaa atatgatgaa aatttactac acactgttgt ttttcttgtt
     4201 ttcgttattg gctttttccc aaacaagaac ctacactact tcacgcacgc agcaagtacc
     4261 ggatatagac ggtcaactga cagacgactg ctggctgaat gccgtctggg gaagtgattt
     4321 tgtgcagttt gaaccccatt ccggtgccca accttctcaa caaactcaat ttgctattgt
     4381 ctacgatgac gataatttat acatagctat aaaagcactc gacgccgaac ccggtaagat
     4441 tgtccgccgc gtttctagac gtgacgatgc tgatggtgat gcggttgcca ttgccataga
     4501 cagttacgaa gataagatga cggcttttgc gtttctggtt accgccgcag gaagtaaaac
     4561 cgactggagg ctaacggagg agaacgacga ggactacagt tgggacccga tttgggaagt
     4621 gagaacttcc ctgcaacccg acggatggta tgccgaaata cgtataccct attctcagct
     4681 tcgtttcgga aagaacaatc aataccgttg gggcctggag gtgatgcggc acgtttatcg
     4741 ccaccaggag cgttcgttct ggcagcctat cgacaaagct tcggccaaat ttataggtaa
     4801 tttcggcgtt ctggatggta ttcgtaacat taatcccaaa aaagacatcg agattgttcc
     4861 ttttgccctt tctcagctta ccctttcgga aaaggaaaaa ggaaatccgt ttgctacggg
     4921 aacccgtaca aaagcgtcag tgggagtgga cggtaaggtc agtattaccc acgacattac
     4981 actgaatttc acggtaaatc ccgatttcgg gcaagtggaa gccgacccgt cggtggtgaa
     5041 cctaaccaat ttcgaaacgt attttgatga aaaacggcct ttctttgtgg aagggagtaa
     5101 tatttaccat tacccgttcg atttgaccaa caacgaacgg aataagcttt tttattctcg
     5161 tcggttggga agagagccac accttagtta cactaaaaaa gagggcgaat atgtgcgtga
     5221 acccgggcga acaaacatcc ttggcgcggt aaaagtttct ggaaaaacac gtgaaggaac
     5281 ttccatcgga atactgaatg cggtgacgca gaaaatgtac agtgaaatag atcatagtgg
     5341 agaacggtcg aaatatgccg tggagccgat gtcaaattat ttcgtgggcc ggctggaaca
     5401 ggatcttgat agcggaaacc ttattgtagg tgggatattt acagccacta accgtaagat
     5461 agatgaagat caattcagaa cgctgcctgg ttcagcgtac accacaggac tgaatttaac
     5521 cagatattgg aaagagaaat cctattttat aacggccagg gtattcttca gccacttgcg
     5581 tggaactcag gaagcgatga ttcaattaca gaaatcgtcg gcaaggtatt gccaacggcc
     5641 cgatgcaaag catttggaac ttgatggaga acgtacatcg ctgagcggtt ttggcggatc
     5701 ggtcagtgga ggtaaagctt cggggcactt taatcttttg ggattcagta gttggagctc
     5761 ccccggactg gaatttaacg atgtcggttt taaacccaat gccgatatgt ttctgaacgg
     5821 gatatgggca gcatggaacg aatggcgccc gaaaggcatc gtgaacgaat atcagtttaa
     5881 tggttcctgg tataacgtga gggatttttc cggaacttcg ctggagtggg ggaacgaagc
     5941 attcgggtac attcagttta ccaattatta cgacattgcc ggagggttga atattaatta
     6001 tccgggatta tccaattcaa tgctgagggg cggacctgca ctcaaaacgc ccggagtaat
     6061 taattcgtgg ttttccgttg aatcggacag ccgaaagaag ttaagcctag aatacgaaat
     6121 gaacttttct catggcttcg agcattcaag gcgttccatt tcttatggat tagacattac
     6181 ctacaaaccc aataaccggc tttcgctttc actttatcca cagtacgtca aagctaccgg
     6241 gcatcaacag tatgtgcgta cggtgacggg agacagtcct gcatatatcc tgtcgtccat
     6301 cgaccaaaat atcgcatcta tgtcggttcg ggtaaattac ggcattactc ccgatatgtc
     6361 actgcagtgg tatgcgcttc cctatctgtt ttcgggagat tattacggtt ttaaaactgt
     6421 aactcatccg cgggcagata aatctagcga tcgattcaca gctatggata cctatcccta
     6481 tgataatccc gatttttatt ttcttcaatt ccgctctaac ctggtttacc ggtgggagta
     6541 caaacccggg tctgtactct acctggtatg gtcgcaagga agaacggtgc aggggaatga
     6601 gggtgtgttc cgttttaggg attacctggg cgatttattt gaggctgctc ctcaaaacga
     6661 ttttctggtg aaagtttcgt acgcgtggat tttttaaccc ggtaaatgct gtctgtacaa
     6721 ttaatgtttg cggattttta gtaagtttgc acttccaaca aaatcaaact tacaatgaaa
     6781 cgaatccctg tttttacagt tttacttgct ttgtgtattc ttactctatc tgctcaagat
     6841 aacgcaagca aaaagagtta tactttttta ttgactggag cttcctttgc gtcgcccaac
     6901 aacggttggt tcgaaatcgg ttgtgaactg tcggatgcca acccgcttaa cagagccatt
     6961 ggcggtgaag ctattgccga tgctgccaac cggattattg acggaacatt atacaccatc
     7021 gaagaactgg aacatattga tgctcttgtg attatgcagg tccacgacag ggatgtcttc
     7081 gacgaatcgc agctaaagcc taactacacc gagtacccgg tgcctttcga ccggagcaat
     7141 tacgccgctg cgttcgacta cgtcatcaaa cggtaccttg ccgattgtta caacctgaag
     7201 tttgacagga aatcgaaata tttcaacagc cggagtggaa aacctgctgt cgttgtgctt
     7261 tgcaccgatt ggcacgacgg aagggtaacc tacaacactt ccgtgagaaa actggccgaa
     7321 aaatggggat ttccggtagt ggagttcgat aaattcatcg ggttctccag gaatgctctt
     7381 catccggtta cgggagaaca aataagccgt ttgtttacgg gtgataaaca agaaattgac
     7441 ggtgaaattt ttggatggca tcccgaaaac gggaaagagc aatacattca acaacggatg
     7501 ggagccgttt ttgccgacac catgcgaaag attttccctg tgaaaccata acctattct
//
LOCUS       DAOSDR010000116         7559 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrimonas sp. isolate AMR_MDS_1826
            NODE_3575_length_7559_cov_4.221882, whole genome shotgun sequence.
ACCESSION   DAOSDR010000116 DAOSDR010000000
VERSION     DAOSDR010000116.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863355
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrimonas sp. (wastewater metagenome)
  ORGANISM  Petrimonas sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Dysgonomonadaceae; Petrimonas.
REFERENCE   1  (bases 1 to 7559)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7559)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:10:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,720
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 35
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7559
                     /organism="Petrimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3575_length_7559_cov_4.221882"
                     /isolate="AMR_MDS_1826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2023866"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1128)
                     /locus_tag="PLI30_05960"
     CDS             complement(<1..1128)
                     /locus_tag="PLI30_05960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008146996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HOI79135.1"
                     /translation="MINETVITDLKQVTIRFSGDSGDGMQLTGTLFSTLSAIFGNEIA
                     TFPDYPAEIRAPQGTLNGVSGFQVRVGSKDIYNAGDKADVLVAMNPAALKVNRQHLKK
                     GSIVIFDTDSFTRKDLEKALFKTEDPFAELNLKDVQPIPVAITSMTKDSLADSGMENK
                     DILRSKNMFSLGIVCWLFNRPIEIAEQLLKDKFKKRPKLVETNIKALTDGYNYGNNIH
                     LTATTYRIDTVETGKGFYTDINGNTATAYGLIAAAEKAGVKLFLGSYPITPATDILQE
                     LSARKDLGVKALQMEDEIAGICTAIGASFAGSLGATSTSGPGLSLKSEALGLAVMTEL
                     PLVVVDVQRGGPSTGMPTKSEQADLFQALYGRNGESPLAVIA"
     gene            complement(1343..2647)
                     /gene="murA"
                     /locus_tag="PLI30_05965"
     CDS             complement(1343..2647)
                     /gene="murA"
                     /locus_tag="PLI30_05965"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005641366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HOI79136.1"
                     /translation="MSSFIIEGGHRLKGEIFPQGAKNEALQVICATLLTDEEVVIENI
                     PDILDVNNLIALLKDMGVEVQKLSPDTYSFRASAIDLEFTKTEDYMKKIAALRGSIMI
                     IGPLLARFGKAIVPKPGGDKIGRRRLDTHFNGIMKLGAELVFDEKKQLFSLSANHLRG
                     QYILLEEASVTGTANLLMAAVFAEGETIIYNAACEPYVEQLSKMLVRMGAKIEGIGSN
                     RLTVKGVTRLGGCRHKLLPDMIEVGSFIGMAAMTASEVTIKNASIENLGIIPESFRRL
                     GIAVQQQGDDLYIPPQEHYTIESFIDGSILTIADAPWPGLTPDLLSVMLVVATKAEGC
                     VLIHQKMFESRLFFVDKLIDMGAQIILCDPHRATVIGLGDRFRLRGMTMISPDIRAGI
                     SLLIAAMSAKGKSIIHNIDQIDRGYQDIDLRLNKLGANIERT"
     gene            complement(2736..3734)
                     /gene="murB"
                     /locus_tag="PLI30_05970"
     CDS             complement(2736..3734)
                     /gene="murB"
                     /locus_tag="PLI30_05970"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019540284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HOI79137.1"
                     /translation="MNIQHDIQLQPYNSFKTKALAKLFAQPSTITELQEILSSYKTEK
                     KLVIGSGCNLFFTRDFDGLIIKPEIHGIRVLEENADWVEIEAGAAEDWDNFVEFCVSR
                     GYSGVENLSLIPGTVGAAPIQNIGAYGAEVKDVITYVKTVEASSGKIESFSNTACNFS
                     YRNSIFKQTRKFVVTSSVFRLQKAFTYVEKYADLNLELKNNFHPSLSQVREAIIRIRT
                     RKLPNHLELPNAGSFFKNPILSKPQKETLLTLLPDAPIYNIGENGFKTSAAYLIEKTG
                     CKGKRKGNVGIYEGHALIVVNYGTENGEEILSFVQDIQNEVQCKFHIMLEPEVWIF"
     gene            complement(3764..4471)
                     /locus_tag="PLI30_05975"
     CDS             complement(3764..4471)
                     /locus_tag="PLI30_05975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007856263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HOI79138.1"
                     /translation="MKDAALYLIPTTLGDTPVNQVLPSYNLRITSDLRHFIVENVRTA
                     RRFLKQCNPEIDIDSLAFYELNEHTDRHRISAYLKPIRQGESVGIISEAGCPAIADPG
                     ADVVAIAQSERIKVVPLVGPSSLLMAMMASGFNGQSFAFHGYLPIESGERIKTLKKLE
                     NRVYSENQTQLFIETPYRNKKLAEDILQHCKPQTLLCIAMNISCEDEYIATRPVKAWK
                     GNLPDMQKKPCVFLIYR"
     gene            complement(4468..5037)
                     /locus_tag="PLI30_05980"
     CDS             complement(4468..5037)
                     /locus_tag="PLI30_05980"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004293950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HOI79139.1"
                     /translation="MNLKSDNKSLHWRVLESEYLHQEPWLTVRKEQLQLPNGNIAPEY
                     YVLEYANWVNTIAITREKKFVLVKQYRHGIRQVCYELCAGVCENSDRSPLESAKRELM
                     EETGYGKGKWSEFICVSPNASANTNLSYCFIAEDVERINDQALEDSEDLTVHLFSLDE
                     VKELLMNGEIIQATMAAPLWKYMAVNKLL"
     gene            complement(5098..5754)
                     /locus_tag="PLI30_05985"
     CDS             complement(5098..5754)
                     /locus_tag="PLI30_05985"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: protein motif:HMM:NF013827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HOI79140.1"
                     /translation="MITFLIIGITSVISIAAFGNYTMMDKFIFRPTEAGRGQWYRFFT
                     YGVLHADYTHLIFNMFTLYFFGGDIERAFKATFGGQTGVFYYLLLYVTALPVSILPTY
                     LKQKNNNSYRGVGASGAVSAIIFAYILINPMNFMGVLFIPVWLPAFLFGTIFLLISVS
                     LDKKQSKGINHSAHITGGLYGIAFMVTVFIAFANINLVSEFADKVKIDSIYHLIRIGY
                     "
     gene            complement(5757..6584)
                     /locus_tag="PLI30_05990"
     CDS             complement(5757..6584)
                     /locus_tag="PLI30_05990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007219409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HOI79141.1"
                     /translation="MKKLVFLLTGILVSLLIHAQQRPDPQPAPDTFEKFKVGMAGYTF
                     VKFDLDKTLEIMQKCDVRYLCIKDFHLPMNSTDEQIAAFHAKLKAKNVTGYAVGPVYM
                     RSQEEIDKAFEYAKRVGVKMIVGVPNYDLLPYVDKKVKEYDFKYAIHLHGPDMPLYPD
                     ADDVWKNVKDLDPRIGMCLDIGHDRRNGKDPVKDLKKYRSRVFDVHIKDVTGATKQGY
                     SVEVGRGILDIPAFVKMLRKVGYEGVCSLEHERNMNDPFLGIAESIGYFRGVIAATK"
     gene            6744..>7559
                     /locus_tag="PLI30_05995"
     CDS             6744..>7559
                     /locus_tag="PLI30_05995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005938306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOI79142.1"
                     /translation="MNFLFESRQPVRYAFIAVATVIALVSLVFSNTLVKGLAKEERNK
                     VEIWAAATELLSKSDESSDMNLVLQILQSNKTIPVILYDKTSNTLSANNIKLPEKDTQ
                     GFLLKKTGEFAVKHDPIELEELNQSVYFDDSFILKRLQIYPYVQLLVISIFIALAFFA
                     LMSSQKAQQNKVWAGLSKETAHQLGTPISSLIAWTEYLKLKGLDPALINEMGKDVYRL
                     EVIAERFSKIGSVSDIKPVTLQDAVKQSVAYLENRISDKVRLIFDFPESSTMVQ"
BASE COUNT         1906 a         1696 c         1696 g         2261 t
ORIGIN      
        1 cgctatcacc gctagggggc tttcgccgtt tctcccgtac agagcctgaa acaaatcggc
       61 ttgttcgctc ttggtgggca ttcccgttga tggaccgcca cgctgtacat caaccacaac
      121 caacggcagc tcggtcatca ccgctagtcc aagtgcttcg cttttgagcg acaaccccgg
      181 acctgaagtg gaggttgcac ccaggcttcc ggcaaagctg gcgccgatgg cggtacagat
      241 tccggcaatt tcatcttcca tctgcaatgc ttttacgccc aggtctttcc ttgcggagag
      301 ttcctgtaag atatcggtgg ccggcgtgat ggggtaggaa cctagaaaaa gttttactcc
      361 ggctttttcg gcggctgcga ttagtccgta tgctgtggca gtatttccgt ttatatcggt
      421 ataaaaaccc tttccggttt ctacggtgtc gatacggtag gtggtagctg tcaggtgtat
      481 gttgtttccg tagttatatc cgtctgttaa tgcttttata ttggtttcga ccagttttgg
      541 tcttttcttg aatttatctt tcagcagttg ttcggcaatc tcaatcggcc ggttaaagag
      601 ccagcaaacg atgcccagcg aaaacatgtt cttgcttcgt aaaatatcct tgttttccat
      661 tccgctgtct gccagtgagt ctttggtcat cgatgtgatg gccaccggaa taggttgaac
      721 gtccttgagg ttaagttctg caaaaggatc ttctgttttg aagagcgctt tctccaggtc
      781 ttttcgtgtg aaggaatcgg tatcaaaaat gacgatcgat ccttttttca agtgctggcg
      841 gttgactttc agtgcagcgg ggttcatcgc gaccaatacg tcggctttat ctcccgcgtt
      901 gtaaatatct ttcgatccta cacggacctg aaaaccagaa actccgttta acgtgccttg
      961 cggtgcgcga atttctgccg gataatcggg aaaagtggcg atttcatttc caaaaatggc
     1021 agacaaagtg gaaaataacg tcccggttaa ctgcatcccg tcacccgagt caccggaaaa
     1081 ccgtatcgtt acctgcttca aatctgtaat tacagtttca ttaatcataa taaatgtctt
     1141 gatttgtaaa tctttaactt ctttgtgtgt tttttcaatc gagtttttga aaaacaagtt
     1201 tctaaacttt tgacaggtta attccgttga taataatacg gtgcaaagta acaaaagagt
     1261 tggggaaaaa gcaaattttg cgttctataa attaattagc cggatgcctt accttgtgtt
     1321 aacagacttc aagcttcctg tttcatgtcc tctcaatatt tgctcccagc ttattcaacc
     1381 gtaagtcaat atcctgatag ccccggtcta tctggtcgat attatgaatg atgcttttgc
     1441 ccttggcgct catcgccgca ataaggagcg agatcccggc gcggatatcg ggcgagatca
     1501 tggtcatccc acgaaggcgg aatcgatcac ctaacccgat aactgttgcc cggtgcgggt
     1561 cacataaaat aatctgagcc cccatatcga taagcttgtc tacgaaaaac aggcggctct
     1621 caaacatttt ctggtggatc agcacgcacc cttcggcttt ggtggcaaca acaagcatga
     1681 cgctcagtaa gtcaggggta aggccgggcc acggtgcatc ggcaatagtc aggatggaac
     1741 cgtctataaa cgattcgatg gtgtaatgtt cctggggcgg aatgtataaa tcatcgccct
     1801 gttgctgaac agcgataccc aacctgcgga aactttcggg aataattccc agattttcta
     1861 tcgacgcgtt ctttatggtt acttcagaag cagtcatcgc tgccattcca ataaagctgc
     1921 cgacctcgat catatcgggc agcagtttgt ggcggcaacc ccccagtctt gtaactcctt
     1981 ttacggtgag ccggttagaa cctatccctt cgatttttgc ccccatcctt accagcatct
     2041 tgctcaattg ctccacataa ggttcacagg cggcattata gataatggtt tctccttctg
     2101 caaacacggc agccatcagc aagttagcgg ttccggtaac cgatgcctct tccagcagga
     2161 tgtattgccc gcgcaggtgg ttggcggaaa gggaaaaaag ttgttttttt tcatcgaaaa
     2221 ccagttcggc ccccagcttc atgattccgt tgaaatgtgt atccaaacgc cgccgcccga
     2281 ttttgtcgcc tccaggtttg gggacgatcg ctttcccgaa tcgtgccaaa agtggcccaa
     2341 ttatcataat tgaaccacgc agggcggcga tttttttcat gtaatcttcg gtcttcgtga
     2401 actccaaatc aatagctgaa gctctgaacg aataggtgtc cgggcttaat ttttgcactt
     2461 ccacacccat atctttaagc aatgctataa ggttgttcac atccaggata tcggggatgt
     2521 tttcaataac aacctcttca tcggtaagca gtgtggcgca gattacctgt aatgcttcgt
     2581 tttttgctcc ctgaggaaat atttcgcctt ttagccggtg tccgccttcg ataataaatg
     2641 atgacatacg ttgattttcg atttgtgcaa atttagggtt ttcttttgaa ataatcgttg
     2701 tttaggaaaa tacggccggc actttctgcc cttcgttaaa aaatccagac ctccggctcc
     2761 agcataatgt ggaatttaca ttgtacctcg ttttgaatgt cctgaacgaa actcagtatt
     2821 tcctccccgt tttcggttcc gtaattcacg acaatcagcg cgtgaccttc gtaaatgccc
     2881 acgttgcctt tccgttttcc tttgcatccc gttttctcga taaggtatgc cgccgaggtc
     2941 ttaaaaccgt tttctccgat attgtatatt ggcgcatcgg gtaggagtgt gagcagtgtt
     3001 tccttttgcg gttttgataa aatgggattt ttgaagaaac tccccgcgtt aggcaattca
     3061 aggtggttgg gtaatttgcg ggtgcggatc cggataatgg cttcccttac ctgggataaa
     3121 gaggggtgga aattgttttt cagttccagg ttcaggtctg catatttttc tacatacgta
     3181 aatgcttttt gcaacctgaa aaccgacgag gttaccacaa atttacgggt ctgcttgaag
     3241 atgctgtttc gataggaaaa attgcatgct gtgttggaga aactttcaat tttgccactt
     3301 gaggcttcca ctgttttcac gtacgtgatt acgtctttta cttcggctcc gtaagcaccg
     3361 atgttttgta tgggggccgc tcctacagtt ccggggataa gcgataggtt ttccactccc
     3421 gaataaccgc gagaaacaca gaactccaca aagttgtccc aatcttccgc tgcccctgct
     3481 tcaatttcta cccaatcggc attttcctct aaaacacgga tgccgtggat ttcgggtttg
     3541 ataatcagcc cgtcgaaatc tcgggtgaaa aacaggttgc atccgcttcc gataaccaac
     3601 ttcttttcgg ttttgtagct ggagagaatt tcctgcaatt cggtaatggt tgacggttgc
     3661 gcgaaaagtt tagccagcgc ttttgtttta aaagaattgt agggttgaag ttggatgtcg
     3721 tgttgaatgt tcattcgggt tatgggttta ttgcttatag cgcctatctg tatataagaa
     3781 atacgcacgg tttcttttgc atatcgggca aattgccttt ccaggccttc accggtcttg
     3841 ttgcaatgta ttcatcctcg caggagatat tcatcgcgat acataacagt gtctgaggtt
     3901 tgcagtgctg taaaatatcc tctgccagct ttttgttgcg gtagggtgtt tcgataaaaa
     3961 gctgcgtttg attttcggaa tataccctgt tctccagttt cttaagtgtt tttatccgct
     4021 cgccggattc aatgggcagg tagccgtgaa aagcaaagct ttgcccgttg aatcccgatg
     4081 ccatcatcgc catgagtaag gatgacgggc caaccagcgg tacaactttt attctttcgc
     4141 tttgtgcaat agccactaca tcagctcccg gatcggctat ggccggacag cctgcttcgg
     4201 agataattcc cacactttct ccctgccgga tgggtttcag gtacgctgaa atacgatgcc
     4261 ggtcggtatg ttcgttcaac tcgtagaaag caagggaatc gatatcgatt tcaggattgc
     4321 attgcttcag aaatctacgt gctgttcgta cgttttctac gatgaaatgg cgtaaatcgg
     4381 aagtgatccg gaggttgtac gatggtaaaa cctggttgac cggcgtgtct cccagcgttg
     4441 tgggaatcag gtataaggcg gcatctttca tagtaacttg tttacagcca tgtatttcca
     4501 taaaggagca gccatggtag cctgaataat ttctccgttc atcagcaact cttttacttc
     4561 atcaagagaa aaaagatgca ccgtaaggtc ttcggagtct tctagtgcct ggtcatttat
     4621 cctttccaca tcctcagcga taaaacagta ggagaggttt gtgtttgcgc ttgcattggg
     4681 agaaacacag ataaattcgc tccatttccc ttttccgtag ccggtttctt ccatcaactc
     4741 ccgttttgcc gattcgagtg gcgaccggtc gctgttttcg cataccccgg cacacagttc
     4801 gtagcaaacc tgtctgattc catgcctgta ctgcttcacc agtacaaatt ttttctcgcg
     4861 ggttatagca atcgtattta cccaattggc gtactccaag acgtaatatt cgggagcgat
     4921 atttccgttg ggcaattgca gttgctcttt tctgaccgtt aaccagggtt cctgatgcag
     4981 gtattcgctt tccaatactc tccaatgcag ggatttgttg tcgcttttca aattcatgga
     5041 tgaacatttt cctgcaaaat tagccaaaaa tgtttataca tgttgtgatt ttacgaatca
     5101 ataacctatc cggataagat ggtatattga atcgattttt actttgtctg caaactcgct
     5161 taccaggttg atgttggcaa atgcgataaa aaccgttacc ataaaagcta ttccgtacaa
     5221 accgccggtt atgtgtgccg agtgattgat ccctttggat tgctttttgt caagtgaaac
     5281 agaaataagc aaaaaaatcg ttccgaaaag gaaagcgggt agccataccg ggataaatag
     5341 gactcccatg aaattcatcg ggtttatgag aatatatgcg aaaattattg ctgaaaccgc
     5401 cccggacgca cccactcccc ggtaactgtt gttgtttttc tgtttaaggt aagtggggag
     5461 aatggatacg ggcagggcgg ttacgtagag cagcaggtaa tagaaaactc ctgtctgtcc
     5521 tccaaatgtt gctttaaaag cacgctctat atcgccgcca aaaaaataga gcgtgaacat
     5581 attgaatata aggtgcgtat agtcggcgtg taatacacca tacgtgaaga aacgatacca
     5641 ttgtccgcga cctgcttctg tgggcctgaa aatgaattta tccatcatcg tatagttgcc
     5701 gaaagcagct atggaaatta ccgatgttat tccgattatc aggaacgtga tcatatttat
     5761 tttgtggcag ctattacccc acggaaatat ccgatggatt cggcaatgcc cagaaacgga
     5821 tcattcatgt tacgctcgtg ttccaagcta caaacgcctt cgtatcctac ttttctgagc
     5881 atttttacaa acgccggaat atccagtatt ccccggccta cttcaacgga atagccttgt
     5941 ttcgtggctc cggtcacatc cttgatatgc acatcaaaaa ctcgggaccg gtattttttc
     6001 aggtctttca caggatcttt tccattacgg cggtcatggc caatatccag acacatcccg
     6061 atgcgtggat caagatcttt tacgttcttc cagacatcat cggcgtcggg ataaaggggc
     6121 atgtctggtc cgtgaagatg aatggcatac ttgaaatcgt actctttcac ttttttatcg
     6181 acgtaaggca gtaaatcata gttgggaacc ccgacgatca ttttcactcc cacacgtttc
     6241 gcatactcaa acgctttgtc aatttcttcc tgcgaccgca tataaacggg gccaacagca
     6301 tagccggtca cgtttttggc tttcagcttg gcgtgaaaag ccgcaatctg ttcatcggta
     6361 ctgttcatcg gcaaatgaaa atctttgatg caaaggtagc gaacatcgca tttctgcatg
     6421 atttccaatg tcttgtccaa gtcaaatttt acaaatgtat acccggccat tcccacttta
     6481 aatttttcga atgtgtccgg cgccggttgc gggtcgggtc gttgttgtgc atggatcaac
     6541 agggaaacca aaattcccgt cagcaaaaaa actaatttct tcatcttgag ttaaattaaa
     6601 aaagtatttc tgtgttcgtg cacaaaaata caaattttat ggagaagcac gaataaaaag
     6661 tgaagtttgt tataaaataa ttgcttattc aataaatcga accttttttc gttactttgt
     6721 aaacaactaa agcaaatgaa cggatgaact ttttattcga atcccggcaa cctgtgagat
     6781 atgcttttat agctgttgca actgtgattg cccttgtttc gcttgtcttc tcgaacacgc
     6841 ttgtaaaagg attggccaaa gaggaaagga ataaagttga gatctgggct gcggcaacag
     6901 agttgttatc caaaagcgat gaaagcagcg acatgaatct ggtattgcag attctgcaaa
     6961 gtaacaagac cattcctgta atcttgtacg ataaaaccag taataccctt tctgcgaata
     7021 acataaaact acctgaaaaa gacactcaag ggtttttgct gaaaaaaaca ggggagtttg
     7081 ctgtaaaaca cgatcccatt gaacttgagg aactcaatca gtctgtttat tttgatgatt
     7141 cttttattct taaacggttg cagatatatc cgtatgtaca gttgttggtg atttctattt
     7201 ttatagcgtt agcttttttt gctctcatga gctcacaaaa agcacagcag aacaaggtat
     7261 gggctggatt gtcgaaagag acggctcatc agttgggaac gcctatctcg tcgttgatag
     7321 cgtggacgga gtatttaaag ctgaaagggc tcgatcctgc tttgataaac gaaatgggta
     7381 aggatgtgta tcggttggag gtcattgccg aacgattttc gaaaatcgga tctgtttccg
     7441 acataaagcc tgtgactttg caagatgcag taaaacaatc ggttgcctat ctcgagaatc
     7501 ggatatccga taaagtgcgg ttgattttcg attttcccga atcttcaaca atggtacaa
//
LOCUS       DAOSDR010000117         7553 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrimonas sp. isolate AMR_MDS_1826
            NODE_3579_length_7553_cov_3.318485, whole genome shotgun sequence.
ACCESSION   DAOSDR010000117 DAOSDR010000000
VERSION     DAOSDR010000117.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863355
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrimonas sp. (wastewater metagenome)
  ORGANISM  Petrimonas sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Dysgonomonadaceae; Petrimonas.
REFERENCE   1  (bases 1 to 7553)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7553)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:10:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,720
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 35
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7553
                     /organism="Petrimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3579_length_7553_cov_3.318485"
                     /isolate="AMR_MDS_1826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2023866"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..471
                     /locus_tag="PLI30_06000"
     CDS             <1..471
                     /locus_tag="PLI30_06000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005648612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SusC/RagA family TonB-linked outer membrane
                     protein"
                     /protein_id="HOI79143.1"
                     /translation="GLNLTAGYKNFDLSMIFTGAAGVYRMINQEVTGFFDGDDSHPAT
                     AWLNAWTPENKNAKMPRIAYSNTSPSISSQVMSSFWIQNASYVRMKNLQIGYTLPKGV
                     VNPIGIENMRVYFSSENLFTLHNMLVNVDPEISSERASSFPLTQTHALGVSISF"
     gene            507..2117
                     /locus_tag="PLI30_06005"
     CDS             507..2117
                     /locus_tag="PLI30_06005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005648613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HOI79144.1"
                     /translation="MKKIVIYMLAVAALAGATSCEGFLDTAPHDALSPSTTWKTEADA
                     QKFLVGCYDGWFSDENLLYWDCASDFGYNFHIHEGWRNIGNGGMTASNAVADFYRFTT
                     IRRCNDFLTNIEPIQFADEKVKNDMIGQVKTIRAYQYYERNRLYGGFPIIDSYATADE
                     AKVPRKSEEEVKKYVFDEIDAAIPLLSDKPASSGTIAKGTALALKMRSALYYGDYQRT
                     IDAAKAIVALGQYELDENYAHVFTVAGKGSKEIIASYQRDKSLYSQWVIGAMYNNADG
                     GWSSMVPTQNLVDTYEMSNGLTKDESGSGYDPKHPFANRDPRMAMTVLYPGMDWQGGI
                     INTLDKTIGGNKNPNDPDDANNASKTALTWAKYLAPKSQYDNMWSTDVSVILFRYAEV
                     LLSYAEAENELNGPSETVYTLLNQVRNRAGMPEVNKAKYGTKETLRELIRRERGVELA
                     GEGIRRADILRWKDANGKMVAETVLNGPLTRVAGTINYQEPDPFKRAVVTGTSKVEDR
                     EFKIHNKYLPIPQGALDKNPELKQNPGY"
     gene            2329..3288
                     /locus_tag="PLI30_06010"
     CDS             2329..3288
                     /locus_tag="PLI30_06010"
                     /inference="COORDINATES: protein motif:HMM:NF040218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BACON domain-containing carbohydrate-binding
                     protein"
                     /protein_id="HOI79145.1"
                     /translation="MHTSTLALSVAALILFFLPGCGKGEIPGGGENKTITLEVSASPI
                     HFAAEGGSKEITVVTNAKSWSVTSSKSWCTVNKGASNFTVTATENKAFAPPEKAILIV
                     AAEGTAKKVTIEVTQDAAVEPAEAFIKPVTDKVIMNYQGGVNGIGIESNVTGWSCRSD
                     QSWCQLEKISDEGINITVDESWTGNIPRQALVTLYGNEGDSLASITVYQDPLPELDLF
                     SSHGNTNIPLPSYGGTIVVTVMTNVTEFTLGYVGEWLQVNKINDTQFTITSTPNNSTQ
                     MRPEQEIIVYASTLTRKLWVYEEEAEEASDNDDYDYNDPTGWD"
     gene            3308..5851
                     /locus_tag="PLI30_06015"
     CDS             3308..5851
                     /locus_tag="PLI30_06015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrillin family protein"
                     /protein_id="HOI79146.1"
                     /translation="MKRTDKTNNLIKSSLLVIGLALLGCTNEPFEQGAAPGDNVLTAT
                     IIDGEETTRTAVIDQPGIKAEVRWEVNDRIGVFGKRGGSNLLFTAGVDDILENGKKAR
                     FRTEDSMPTGEVTAYYPYREGTSIGNNSELTLTFPAIQPYTLKERVSQPYGPANFMAG
                     KGSASGIGFRNLFALLKVGYSGTEGDRLRKVHFRDLSERPVSGSFTVAWNEDLPEARF
                     PESGSGPALILTVDCGEGLELDGTLHHFYLFIPPRDYTKGFEVKFVMDDGREVIKTIG
                     TTSGKKIERSRVYPVGETVTASDEAEYKLQEDATLVDADRMDYIVDARVASYDKWVGE
                     VKFETHTLFMTVKKEFEPRMGEILLFNTPSKLFPEGYIGRIVSVGNPESEYIAVVAEP
                     VNDITEVFEELSLGEPIWNEDGSLNGEGGVELELGAYLSRVETPDGEAVSFTRNGSQI
                     ELSMPATRASGRKNYNYSSGNMSFTLEPGDNSEIEVSASMDLSMRLLVGIFDKKLHYF
                     HASISPDIQLSADMTVQVEADAVDKSFHLLTAYFAPIPVPPLTIVPVIHFYGVAGVGG
                     EVEIRAGMSYSAAMDLGFSYNSNEGFRTRSTTRASKFSENGLSFTGGELSSSVHTTAG
                     VRVDAGIKVWGVMEALCSADARMKLGYYWDVFEQTQKMSLNTESKIKGSLTTLGGVFN
                     YETDELTLEGDPIWERYFSPLCDLNASDVNLSADSTQVHCKFKVMRHLLQPVQIGAIL
                     YSGVPYGYLESGMPYGFKPTEKIMEVDFGQSYIGSGMSRREDNRVVYDDFELFEHTFP
                     FTPEPGGYYGILPAKGTVGSGNFGDMPVNSTDYYGLRYFTFRGNKLPAD"
     gene            complement(5868..7037)
                     /locus_tag="PLI30_06020"
     CDS             complement(5868..7037)
                     /locus_tag="PLI30_06020"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HOI79147.1"
                     /translation="MTHYFFTVMPLFVVFFWLILFLLDFRRNDTAKRFLTLFLGVALV
                     NYLTHWFYFNHNYSVYRLLDSVWVFTSLAVYPLYYYYIRLLTEDLKLDWKWGWILIPA
                     ILLSSFSTVLYLLMSSAELDVFTHEVLYHNREYRVGYPLLVRLQVMRLVLFKVIFAME
                     VLLTLYFGLRLIRLFNEKVHAFYSNVENREISKIRTLLFFLVLTALISLVSNVVGKHF
                     FSDHPSLLALPSLAHSMALFGISYVGYRQHFSIRDLCQDQQEGECCCGVESESEAMTG
                     MEYDQLLSKLETLFEEDQIFKNPELRLNDVAQELGTNRTYLSRLIRHRRDMTFCDFVN
                     DYRVRYAEAVLSSPEQFRLTIDEVAFNSGFSGSSSFYRAFVKKNGIPPGKFRKQV"
BASE COUNT         2141 a         1814 c         1879 g         1719 t
ORIGIN      
        1 gggctgaacc ttacagcagg ttataagaat tttgatcttt ccatgatatt cacgggtgca
       61 gccggtgttt accgcatgat caaccaggaa gtaaccggtt ttttcgacgg tgatgattca
      121 catcccgcaa cagcatggtt aaacgcctgg actcccgaaa acaaaaatgc taaaatgccg
      181 cgcattgcct attcaaacac gtcgcccagc atttcatcgc aagtaatgtc atcattctgg
      241 atccagaacg ccagctacgt acgtatgaaa aacctgcaaa tcggttacac attgcctaaa
      301 ggcgttgtaa accccatagg cattgagaat atgcgtgttt atttttcgtc ggaaaatctt
      361 ttcacgctcc acaacatgct ggttaatgtt gatccggaaa tctcgagcga acgtgcatcg
      421 agctttcctc tcacgcaaac ccatgctctt ggcgtaagca tctcttttta atatgtatga
      481 cccatttaaa aacgaaaaaa taaaatatga aaaaaatagt aatctatatg ctagctgttg
      541 cggcactggc cggagcaaca tcttgcgaag gttttttaga taccgcgccg cacgatgcct
      601 tgtcaccgag tacgacctgg aaaaccgaag cggacgccca gaaatttttg gtcgggtgct
      661 acgacggatg gtttagcgac gaaaatctcc tttactggga ttgtgcttcc gatttcggat
      721 ataacttcca catccacgaa ggatggagaa acataggaaa cggcggtatg accgccagca
      781 atgcggtggc tgatttttat cgcttcacta ccattcgccg ctgcaatgac ttcctgacaa
      841 atatagagcc gattcaattt gccgatgaaa aagtaaagaa cgatatgatc gggcaggtaa
      901 aaacaatccg ggcctatcag tattacgaga gaaacaggct ctatggcggt ttccccatca
      961 tcgactcata cgcaacggcc gatgaagcga aagtaccccg caagtcggaa gaagaagtga
     1021 aaaaatatgt cttcgacgaa attgatgcgg ctattccatt attaagcgac aaaccggcct
     1081 caagcggcac cattgccaaa ggtaccgcat tagcgctaaa gatgcgttcg gcactgtatt
     1141 acggtgacta tcagcgaacg attgacgcgg ccaaagccat cgttgcttta ggccaatatg
     1201 aactggacga aaactacgct catgttttca ccgttgccgg gaaaggctca aaagaaatca
     1261 tcgcttctta tcagcgggat aaaagcctct actcacaatg ggtaatcggt gccatgtaca
     1321 acaacgccga tggaggctgg tcgtcaatgg ttcccactca gaaccttgtc gacacttatg
     1381 aaatgagtaa cgggcttacc aaggacgagt ccggcagcgg atacgatccc aaacacccgt
     1441 ttgccaaccg tgatccgcgt atggccatga ctgttctcta cccgggtatg gattggcaag
     1501 gcggtattat aaacacgctg gataagacca ttggcggcaa taaaaacccc aatgaccccg
     1561 atgacgcaaa taacgcctcc aaaacggcat tgacctgggc aaagtatctt gctccgaaat
     1621 cgcagtatga caatatgtgg agtaccgatg tcagcgtaat cctttttcgc tatgccgaag
     1681 tactgctgtc ttatgcggaa gcagaaaatg agttaaacgg tccgtctgaa acagtttata
     1741 ccctgctcaa ccaggttcgt aaccgagcag gaatgcccga agtgaacaag gctaaatacg
     1801 ggacaaaaga aaccctacgc gaactcatcc gtcgcgaacg tggcgttgaa ctggccggtg
     1861 aaggtattcg cagagcggat atcctccgct ggaaagatgc caacggtaaa atggtggctg
     1921 aaaccgtact gaacggtccg ttaacgaggg tagccggcac tataaactac caggagcccg
     1981 acccattcaa acgagctgtc gttaccggta cttcaaaagt agaagaccgc gaattcaaga
     2041 tacacaataa gtatttacca ataccgcaag gtgctctcga caaaaacccg gaattgaaac
     2101 agaatcccgg ttattaattc tctatttttt atattgttta acaaggccgc tcagttgagc
     2161 ggccttgttt ttttgcccct atttttgtcg gttttgtcca tttttttgtg aaaacaaaag
     2221 atttttttgt ctatttggga aaaaactccg gccggttaac actatttttg ctgccacgct
     2281 acggcgccag ccgttgaata aatgaagcat caatacctgt aagataatat gcatacatca
     2341 acactcgcac tatctgtcgc ggccttgatt ttattttttt tgcccggttg cggcaagggt
     2401 gaaatccccg gtggagggga aaacaagaca atcaccctgg aggtatccgc ctcacccatc
     2461 cacttcgccg ctgaaggagg aagcaaggag atcacagtcg taacgaatgc caaaagttgg
     2521 agcgtcacat ccagcaagag ctggtgcacg gtcaacaagg gagcctccaa cttcacggta
     2581 actgcaactg aaaacaaggc tttcgcccct ccggagaaag caatcctaat cgttgcagct
     2641 gagggaaccg ccaaaaaggt gaccatcgag gtgacgcagg atgcggccgt cgaaccggcg
     2701 gaagctttca tcaaaccggt taccgataag gtaatcatga attatcaggg gggtgtaaac
     2761 ggaattggta tagaatccaa cgtaaccggg tggagttgcc gttccgatca gtcctggtgc
     2821 cagcttgaaa aaatttcgga cgaaggcatt aacatcaccg ttgatgaaag ttggacgggc
     2881 aacattcccc gacaagcctt ggtcaccctc tatgggaatg agggcgactc gctcgcctcc
     2941 atcacggttt accaggatcc tttgccggag ctcgacctct tctcctctca tgggaacacc
     3001 aacatacccc tcccttcata cggcggcacc atcgtggtca cggtgatgac caacgtgacc
     3061 gagttcaccc tgggatatgt tggagagtgg ttgcaggtga ataagatcaa cgatacgcag
     3121 ttcaccatca cctcaacgcc caacaacagc acccagatga gaccggagca ggagatcatt
     3181 gtctatgcca gcacactaac acgaaaacta tgggtatacg aagaagaggc ggaagaggct
     3241 tccgacaatg atgattacga ttacaatgac cccacgggat gggattaacc acaaacagca
     3301 ccgcatcatg aagagaacag ataaaacaaa caacctgata aaatcatccc tgctggtgat
     3361 tggcctcgca ctactgggat gtaccaacga gccttttgaa cagggagcag ctcccggcga
     3421 caacgtattg acagctacca tcattgacgg tgaagaaacg acccgaaccg cagtgattga
     3481 tcaacccggc atcaaggcag aagtccgttg ggaggtgaac gaccggatcg gcgtcttcgg
     3541 caagagaggg ggaagcaacc tgctcttcac tgccggtgtg gatgacatcc tggaaaacgg
     3601 taaaaaagcc cggttcagga cggaagacag catgcccacg ggcgaggtga cggcctatta
     3661 cccttatcgg gagggaactt ctattggcaa caacagcgag ttgacgctca ccttcccggc
     3721 catacagcct tataccctca aggagcgggt gtcgcaaccc tacgggccgg ccaacttcat
     3781 ggccggcaag ggcagtgcgt cgggcatcgg cttccgcaac ctctttgcgc tgctcaaggt
     3841 gggttacagc ggtactgagg gtgaccgtct gcgcaaggta catttccggg atctctcgga
     3901 gagaccggtt agtggttctt tcacggtggc ctggaacgag gatctcccgg aagcacgctt
     3961 cccggagagc ggcagcggcc cggcactcat ccttaccgta gactgtggtg aagggctaga
     4021 gctcgatggc accctacacc atttctatct ctttatccct ccccgcgatt ataccaaagg
     4081 gtttgaggtg aaattcgtga tggatgacgg cagggaagtg ataaagacga tcggtacgac
     4141 atctggaaaa aagattgaac gcagcagggt ctacccggtg ggcgagacag tcaccgcatc
     4201 ggacgaggcg gaatataaac tgcaggagga tgctaccctg gtcgatgctg acaggatgga
     4261 ttacatcgtg gatgcccggg tggcgagcta cgacaagtgg gttggtgagg tgaagttcga
     4321 gacccatacc ctctttatga ctgtgaaaaa agaatttgag cccaggatgg gtgagatatt
     4381 gcttttcaat actccctcca aattgttccc ggaaggatat atcggcagaa tcgtctcggt
     4441 gggcaacccg gaaagcgaat acatcgccgt ggtggctgaa ccggtgaacg atatcaccga
     4501 ggtatttgag gagctctcgc tgggagaacc gatctggaac gaggatggct ccctcaatgg
     4561 agagggtggt gttgagttgg aactaggggc ctatctcagc cgggtggaga cgcccgacgg
     4621 ggaagcggtg agcttcaccc ggaacggttc acaaattgaa ctttctatgc ctgcaacaag
     4681 ggccagtgga agaaaaaact acaactattc ctccggcaac atgagcttta cgctggagcc
     4741 tggcgacaac tccgaaattg aggtttcggc cagcatggat ctcagcatgc ggctgctggt
     4801 aggcatcttc gacaagaaac tgcactattt ccatgccagc atatcgcccg acatccagct
     4861 ttcggccgat atgaccgttc aggtggaagc cgatgcagtg gacaaatcgt tccacctact
     4921 aaccgcctat ttcgccccca tccccgtgcc gccgctcacc attgtaccgg tgatccattt
     4981 ttacggtgtt gccggtgtgg ggggagaagt agagatcagg gcaggtatga gctacagtgc
     5041 cgcgatggat ctcggattct cctacaacag caacgaggga ttcagaaccc gcagcaccac
     5101 tcgggcatcc aagttcagcg agaatggcct ctccttcaca gggggtgagt tgtcgagcag
     5161 cgtgcacacc acggcaggcg taagggtaga tgcgggcatc aaggtatggg gcgtgatgga
     5221 agcgctttgc agtgccgatg cacgcatgaa gttgggatac tactgggatg tctttgaaca
     5281 gacccagaag atgtcactca acaccgagag caaaatcaag ggatcgctca ccacgctggg
     5341 aggtgtcttc aactacgaga ccgatgagct gacgctggaa ggagatccca tctgggagcg
     5401 gtacttctct cccctatgcg atctcaatgc cagtgatgtc aacttgtcgg ccgacagcac
     5461 acaggttcat tgcaagttca aggtgatgcg ccacctgttg cagccggtgc agatcggggc
     5521 catcctatac agcggtgtac catacggcta tttggaaagc gggatgccgt atggcttcaa
     5581 acccacagaa aagatcatgg aggttgattt cggacaaagt tacattggat cgggcatgtc
     5641 acgacgggag gacaacaggg ttgtgtacga tgatttcgag ctgtttgaac atacctttcc
     5701 cttcacgccc gagccgggtg gttattacgg catcctgccg gctaaaggaa ctgttggcag
     5761 cggaaatttc ggggatatgc cggtaaacag cacggactac tatggactca ggtatttcac
     5821 ctttcgggga aataagctcc cggccgatta atgttgcttt tgcaccccta aacctgtttc
     5881 ctgaatttgc cgggtgggat tccgttcttc ttgacaaaag cgcggtagaa cgagctgctc
     5941 ccggaaaaac cggagttgaa agcaacctca tcgatagtca ggcggaactg ctccggtgaa
     6001 gagagcactg cttcggcgta gcgaacgcgg taatcgttca caaaatcgca aaaggtcatg
     6061 tcccgcctgt ggcggatcag cctggacagg taagtgcggt tggttcccaa ctcctgggcc
     6121 acatcgttca accgcagctc ggggttttta aagatctgat cctcctcgaa aagggtttcc
     6181 agcttcgata ggagctggtc atattccatc ccggtcattg cctcactctc cgactcaacg
     6241 ccacaacagc actccccttc ctgctgatcc tgacacaggt cacggataga aaaatgttgc
     6301 cggtaaccca catagctgat accgaaaagg gccatcgagt gagcaaggga gggaagggcc
     6361 agaagcgagg gatgatctga aaaaaaatgc ttccccacaa cattggatac caaagaaatc
     6421 agggctgtca aaaccagaaa aaacaacaac gtcctgattt tggagatctc cctgttttcc
     6481 acgtttgaat agaaagcgtg tactttctcg ttgaaaagtc tgatcagacg aagcccgaaa
     6541 tagagggtca gcaaaacttc catggcaaaa atcaccttga acaggacgag cctcattact
     6601 tgaagacgga caagcaatgg gtaaccgacc ctgtactccc ggttatgata cagtacctca
     6661 tgtgtgaaca catcaagctc agccgaactc atcagcaggt agagtacagt ggaaaaggag
     6721 gagagcagaa ttgcagggat caatatccat ccccacttcc agtcaagctt caaatcctcg
     6781 gtcaacagac gaatatagta gtaatacaac ggataaaccg ctagggaagt aaacacccat
     6841 acgctgtcga gcaagcgata aaccgaatag ttgtggttga aataaaacca gtgtgtaaga
     6901 taatttacca aagcgactcc taaaaagagt gtgagaaatc gctttgcagt atcgtttctt
     6961 cggaaatcca gcaaaaagag aatcaaccaa aagaaaacaa cgaacagggg catcacggta
     7021 aaaaaatagt gtgtcatttg tgttaaaaga tctgtttttg taaagttaag aatattttac
     7081 tgttttggga aaagacaccg gtaaagcatt tcattttttt ataatttgaa aacgttcttt
     7141 ttaaaaaatc aatcaacaac ggaaagcaaa ttatttttcg ccgcgttaac ggaattcaat
     7201 aggaacagaa tgtgtttgaa aagtagggaa gtaattgacc gtgaagaatt agccgatatt
     7261 tagtaaatat ttttaactta aaaattcatt gactacgtat atttaattaa aacaggggtc
     7321 atatattaaa ttcagcatta tttatgctgt aaaacatatt tatttttgct aatatatcaa
     7381 aaaaacaatt tacctttgca tcatcacagt acagttcagg atggtttatg taatacttta
     7441 cgtcttttaa aaatacttac tgctatgaga aaattttaca ggacttaatt caagttgtaa
     7501 atccttaata aatagagact caaaggcaat tttgcagaga ctctctaatt acc
//
LOCUS       DAOSDR010000118         7545 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrimonas sp. isolate AMR_MDS_1826
            NODE_3584_length_7545_cov_3.552737, whole genome shotgun sequence.
ACCESSION   DAOSDR010000118 DAOSDR010000000
VERSION     DAOSDR010000118.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863355
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrimonas sp. (wastewater metagenome)
  ORGANISM  Petrimonas sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Dysgonomonadaceae; Petrimonas.
REFERENCE   1  (bases 1 to 7545)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7545)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:10:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,720
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 35
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7545
                     /organism="Petrimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3584_length_7545_cov_3.552737"
                     /isolate="AMR_MDS_1826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2023866"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..536)
                     /locus_tag="PLI30_06025"
     CDS             complement(<1..536)
                     /locus_tag="PLI30_06025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004315733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding protein"
                     /protein_id="HOI79148.1"
                     /translation="MGNPKAFITIPRKEAGYRPVLERVTDFEEVEQALSQEERKVQAS
                     RCMDCGIPFCHWACPLGNRMPEWQDMIYKGKWKEGVEILHETNNFPDFTGRVCPAPCE
                     KSCVLALHEAPVTIRENEASVTEIAFLEGMIKARPPRHRTGKKVAVVGSGPAGLAIAQ
                     QLNRKGHAVTVFEKDKSPG"
     gene            complement(574..5112)
                     /gene="gltB"
                     /locus_tag="PLI30_06030"
                     /pseudo
     CDS             complement(574..5112)
                     /gene="gltB"
                     /locus_tag="PLI30_06030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444622.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase large subunit"
     gene            complement(5182..7377)
                     /locus_tag="PLI30_06035"
     CDS             complement(5182..7377)
                     /locus_tag="PLI30_06035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019538860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamate-ammonia ligase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase III"
                     /protein_id="HOI79149.1"
                     /translation="MSTLRFSAIVEASKRLPVMEEAPKGQPSDYYGKYVFNRKQMAKY
                     LSRDTIKVIVDAIDEGITLPREIAEHVAAGMKMWAMEMGASHYTHWFQPLTDGTAEKH
                     DSFIDYVDAPGGDIIEKFSGKLLAQQEPDASSFPSGGIRNTFEARGYTAWDPSSPAFI
                     VDDTLCIPTVFISYTGEALDYKTPLLKALSAVDKAAVEVCRLFDPKVNKVYSYLGWEQ
                     EYFLVDQALYDARPDLNLTGRTLMGHESAKNQQLEDHYFGSIPPRVAAFMREVEFEAY
                     KYGIPLKTRHNEVAPNQFELAPVFGETNLSIDQNLLVMSLMHRLAAKHHFRLVLHEKP
                     FAGVNGSGKHNNWSLGTDTGVGLFTPGKTARENLQFIVFIVNTLMAVYKNNGLLKASI
                     MSAQNAHRLGANEAPPAIISVFLGTQISAVLDKIEESSEDDDIAVDEFQKMRLGVAHI
                     PEVLIDNTDRNRTSPFAFTGNRFEFRAVGSSANCSSAMTALNASVAMQLIEFKKEIEV
                     KIKTGMKKEQAIYDTLRLYIKACKNIRFDGNGYSDEWKEEAQKRGLNCETSVPLIYDT
                     YISRESVELFETVGVLNERELHARNEVKWETYTKKIQIEARVLGDLSINHIIPVATQY
                     QTMLLDNVYKLRGVFDEKQANELNKDDLALIVDIARHISGIKTNVDAMVEARKVANKI
                     DNEREKAVEYHDKVLPFFDVIRYHIDKLELIVDNQMWPLPKYRELLFIS"
BASE COUNT         1809 a         1933 c         1704 g         2099 t
ORIGIN      
        1 cccgggcttt tgtctttttc aaaaaccgtt accgcatggc cttttctgtt gagttgttgc
       61 gcaatagcca gtcctgccgg acccgaaccg acgacggcta cttttttccc tgtccgatgc
      121 cggggaggac gggcttttat cattccttcc aaaaaagcga tctccgtaac cgatgcttcg
      181 ttttcgcgaa tggttacggg cgcttcgtgc aaggcaagca cacacgattt ttcgcaggga
      241 gcaggacaaa cccgcccggt aaaatcgggg aagttattgg tttcgtgaag aatctccact
      301 ccttctttcc attttccctt gtaaatcata tcctgccatt caggcatacg gttgcccaac
      361 ggacaagccc aatgacagaa aggtattcca cagtccatgc aacgggaagc ctgcactttc
      421 cgctcttcct ggctcaaggc ctgctccact tcttcaaagt ctgtaactct ctccaggaca
      481 ggcctgtatc ccgcttcttt gcggggaatg gttataaatg cttttggatt gcccatttta
      541 tattgattta taccggttta caccttgcag tgatcaccgg gcttctgtta tagaggttat
      601 tttatttttc ttgtccaact ccaccagctt ctcctcctgc aatactttct tatactcgat
      661 cggaatcact ttgatgaatt gctccacgct ttttgaccag ttatccagga ttcttttcgc
      721 cattgggctg ttggtatatt tgtagtgcgc tgaaataagg ccataaagtt ccttttggtc
      781 gtccgcttcc tcaatgagtg aaagttcagc catttccatg ttacagaaat agtcgaaatt
      841 gcccttttca tcgtagacat aagcaacacc tccactcatg cccgcagcga aatttcgtcc
      901 ggtaggccca aggacaaccg tacggccacc ggtcatgtat tcgcaacaat gatccccggc
      961 cccttccaca acggctatag cgccggaatt gcgcacgcag aagcgttcac cggccactcc
     1021 gttgatgtaa acctcacccg aggtagcgcc gtagagcaag gtatttccgc aaagtatgtt
     1081 ctcttcgggc ttaaacgtgc ttcccggggg tgggacgaca atgattctcc cgcccgaaag
     1141 gccttttccc aggtaatcgt tggcatcgcc ttcgatgcga aaggtaactc cttttgccag
     1201 aaatgccccg aaactctgcc cggctgaacc ttcaaacgta gcttttatcg tatcaccggg
     1261 taatccggcc tgcccgtagc gcctggctat ttcgccggaa agcatggctc ctgcagcacg
     1321 gtcgatattc cttatcttca gctttatttc aacaggcata agtaaatcca gcgcgggtga
     1381 cgatctgcga atcaactccc tgtcaaggac gtcgcagatc ttatgttctt gatccgaaat
     1441 tttatgtaac gcattagggg taacgtctcc agggaaaaat ataatttttg aaagatcgat
     1501 cttacctgtt tttggaaaat cgggaaactg cttcctctcc aatagatcgg cccgtccgac
     1561 agcttcttcc agtgttctgc agcccagttg agcaaggtgc tcacgggttt cctaggcaag
     1621 aaacgtaaag aagttcacca aatattcgta tctccctaca aatcgtttgc gaagttcagc
     1681 gttttgagtg gcaaccccta ccggacatgt gttcaggtgg cattttcgca tcatcacgca
     1741 acccaataca atcagggcac tggtggcaaa tccgaactcc tcggctccca gcagcgcggc
     1801 tagaatgatg tcgcgaccag tctttacctg tccgtcagcc tgcagacgga caactccccg
     1861 caaattattc atcacgagcg tttgctgtat ttcggccagc ccaatctcta acggcaaccc
     1921 cgcatgcttg atggaactgg cagggcttgc ccccgttccg ccttcgctac cgcttatcag
     1981 gatcaaatcg gctttagctt tggcaacacc agccgcgatg gtacctaccc cactctccgc
     2041 aaccagcttg acgctcacaa cagccgaagg attgatgttt ttcaaatcga aaataagctg
     2101 cgccaggtct tcgatagagt aaatatcatg gtgaggtggc ggtgaaatca gtgaaattcc
     2161 gggaatggaa tgccttgtcc tggctatgat tttatccacc ttatagccgg ggagctggcc
     2221 tccttcaccc ggcttggctc cctgtgcaat ttttatctga agctcatcgg cattcaccag
     2281 gtattcggcg gtaacgccaa aacgtccgga ggcaacctgc ttgatggccg aacgggtact
     2341 taatccgtcg tcccgcttct tgtatctttc gggatcctca ccgccctcgc ccgtattgct
     2401 tcttcctccg atgatgttca tggcaatggc catcgcttcg tgtgcctccc ggctgatgga
     2461 gccgtacgac atggcacctg tacaaaaccg cttcatgatg tttgcggccg gctccacctc
     2521 cgaaatgtca atcggattcc gtttgtaatc gagcatatcc cttatgaaag ccggattggg
     2581 tttatcgtcc accgttcggg tatattcttt aaacaggccg tagttgttgg ttttggttgc
     2641 aatctgcaaa cgggaaacag tttcggggtt ccaggcatgg tattccccgt tgttgcggta
     2701 agcgtaaata cccaggttct ccaggcgaga cgccccgtta tcggcctcgt tgaatgattc
     2761 gaaaaatggc agcagcacct cgtgggcaat gtcgtccata tcgatgcctt ctatctttga
     2821 cgaaattccc ttgaaataag tattcagtac gcccgaagag atgccgatgg cttcaaaaat
     2881 atgtgctcca cggtaactcc tcagcgtcga gtttcccatt tttgacaaaa ctttaagcag
     2941 tcctttgttg accgacttga tgtagttttt ctttgccgtt tcgaaatcta gctggatatc
     3001 gcctgttttc acttttttcg ccagcacgcc aaacgcgagg tagggattta tagcgttagc
     3061 tccgaagccg aacaacaagg caaaatgcat cacttcccgg ggttcggcac tttccacgac
     3121 gatatcaatc tgaatcctct tccttttttc caccaaataa tgatgcacgg cagaaaccgc
     3181 cagcagggaa ggaattggag cgtgattctt gtcaactccc cggtcgctta agacaatgta
     3241 atttttccct tcatctacgg ctctttctac caaaaggcat agctcgccga tggctttttt
     3301 taatccgtca gcaccttcct cagggttgaa aagcatcggc aagacagcgg ttgaaaatcc
     3361 tttatacctc agattcaaca ggatatcgaa ctgcgtattg gtcaggatcg gacttttcac
     3421 tttcacgatt ttggataagc ggggaatctc atccagcaag ttttgatgaa ttgcccccag
     3481 atacccggtg agcgacatca ccaattcctc ccggatcgga tcgattgggg gattggtaac
     3541 ctgtgcgaat tgttgccgga aatagttgaa caggcgttgc ggtttccggg aaaacagcgc
     3601 cagcgaggcg tcgttgccca tggacgaaac aggctccttg ccttcgttgg ccatcgggac
     3661 aatcaggttc tcaatatctt cttccgaata gccaaaggcg gtcagtaaag tattcaggtg
     3721 aggaatttca ttgttcacgc tccgtcccga ggaaatctct tcgaggttac tgcagttttt
     3781 attgagccaa ttcctgtatg gaaaggcttc ggcaagcgtt tttttcaatt catcgtcgga
     3841 gaaaatgcgg cctgttttag tatccaccat caacattttc ccgggacgaa gacggccccg
     3901 tgcttttatt ttatcgggag caatttcgag cacgccggtc tcggaagcaa tgatcagttg
     3961 atcgtcatgt gtaacagtat accgtgccgg cctaagtccg ttcctgtcca acattcctcc
     4021 ggcaaactgc ccgtcgctga aaagcaacgc agccggtccg tcccacggtt ccatcaacat
     4081 actgtggtat tcgtaaaacg ctttcagatc tccggaaatc ggatttttgt cgttgaagct
     4141 ctccggcaca agcatggcca aggcatgggg aaggcttttg ccggacatca cgagaaactc
     4201 caacacgtta tccagcgatg cgctgtcgct catagcaggc tgaacaatgg ggaacaagtc
     4261 ctgtatatcc cccaggacac cggatttcaa aacactttcc cggctttcca tccattgccg
     4321 gttgccccgg atagtgttga tctcgccatt gtgtccaagc atgcggaaag gctgcgccaa
     4381 gtcccaggta gggaaagtat tggtgctgaa acgagaatgg accaaggcca cccggcttgt
     4441 aaagttcgga tttgaaagat cggggaaata ctcccgcagt tgcagggagg tcagcattcc
     4501 cttgtaaatc atctgcttgg aagagaggct gacgatgtaa aaactgcggt cggccgctgt
     4561 accggactgc aaaatagttt tctcaatttt tttgcgcagg atataaagtt tacgttccaa
     4621 ttcatcctgc ggataacttc cggtaataaa aacctgcttg atatcgggct cgttggagcg
     4681 ggaaatctcc cccaaacagg tagaatttac aggcacgtcc cttacggcaa gcagatgcaa
     4741 atcctctttc acggtgagtt tttgaataag atcaatgcat tcaccggctt tttgcttatt
     4801 tttcggaagg aaaaccaggc cggtcccata tttgccttta gagggaacct caattccttg
     4861 aagcaaaata aactcgtggg ggatatgaac gagaatacct gctccgtctc cggttttgtt
     4921 gtctgcactt tccgcccccc ggtgcaccat attttcgagc acctgaagtc cgttttctac
     4981 cgtttcgtgc gatttgtctc cgttcaacga gactaccaac ccaacgccac aggcatcgtg
     5041 ctcattttga aaagaataaa gggttttacg ctcgtaatca gctatatact gtaaaggatt
     5101 gtcgataaac attgtattcg tattttttta aatccgtttg tccaaaaaga aaaacaggtt
     5161 gaaacagcat ggaaggcagc ttcaactgat aaataacaat tcgcggtatt tgggcagggg
     5221 ccacatctgg ttatccacga tcagttccag cttatcgatg tgataacgga taacgtcgaa
     5281 aaacggcaat accttatcgt gatattcaac ggctttttca cgttcgttat caatcttatt
     5341 ggctactttc cgggcttcta ccatcgcatc cacattggtt ttgattccgg agatgtgcct
     5401 ggcaatgtcc acgattaagg cgagatcgtc cttattcagt tcgttcgcct gtttttcgtc
     5461 aaacacgccg cgaagtttgt acacattatc cagcagcatc gtttggtatt gcgtggccac
     5521 cggaatgatg tgattgatgg atagatcacc gagtacgcgg gcttcaatct gaattttttt
     5581 ggtgtaggtt tcccatttta cttcgtttcg cgcatgaagc tccctttcgt tcagcactcc
     5641 gacggtttca aacaattcca ccgattcccg gctgatatag gtgtcataaa tcagcggaac
     5701 acttgtttcg caattcaatc cccgtttttg ggcttcttct ttccactcgt cggaataccc
     5761 gttcccgtcg aaacggatgt ttttacatgc cttgatataa aggcggagcg tatcgtaaat
     5821 cgcttgctct ttcttcatcc cggtttttat tttaacctcg atctcttttt tgaactcaat
     5881 caactgcatg gctaccgaag cattaagagc agtcattgct gaagaacagt tagccgatga
     5941 tccaacggca cgaaattcaa aacggtttcc cgtgaaggca aacggtgatg tgcggttgcg
     6001 gtccgtattg tcgatcagca cttcgggaat gtgggctacg cccagcctca tcttctgaaa
     6061 ttcatctacc gcaatgtcgt cgtcttcgga gctttcttca attttatcga gaacagcgga
     6121 aatctgtgtt ccgagaaaaa cagaaataat tgccggaggc gcttcattgg ctcctaaacg
     6181 gtgtgcattc tgagccgaca taatggatgc cttgagcaat ccattgttct tgtaaaccgc
     6241 catcaacgta ttgacaataa atacgataaa ctgcaggttc tcacgagcgg tctttcccgg
     6301 tgtgaacaat cccactccgg tatccgttcc cagcgaccag ttgttgtgtt tgcctgatcc
     6361 gtttacgccg gcaaaaggtt tttcgtgaag aacaagcctg aagtgatgct tggcggctaa
     6421 cctgtgcatc aacgacataa ccagcaggtt ttgatctatg gaaaggttgg tttccccaaa
     6481 aacaggggcc agttcaaact ggttgggggc aacttcattg tgccgtgttt ttaacggaat
     6541 accgtacttg tacgcttcaa actccacttc ccgcataaat gctgcaaccc gggggggtat
     6601 agaaccaaaa tagtggtctt cgagctgttg gttcttagcg ctctcgtgcc ccatcagtgt
     6661 tcttccggtg aggtttaaat cgggacgggc atcgtacaaa gcctggtcga cgaggaagta
     6721 ttcctgttcc cagccgaggt aggaataaac tttatttact ttggggtcaa acagccggca
     6781 cacctctacc gctgctttgt caacagcgga taaggccttt agcaaaggag tcttatagtc
     6841 gagcgcttca cccgtatagg aaataaaaac cgttgggatg caaagggtat cgtcaacgat
     6901 aaaagcaggc gatgatggat cccatgcggt atacccccgg gcttcaaaag tgttccggat
     6961 accgccgctg ggaaaacttg aggcatccgg ttcctgctgt gccagtaact tgccggaaaa
     7021 tttttctata atatcgcctc ccggcgcatc aacatagtca ataaagctgt cgtgcttctc
     7081 cgccgttcca tccgtcagcg gttggaacca atgcgtgtag tgagaggctc ccatttccat
     7141 tgcccacatt ttcattcctg ctgcaacgtg ttctgcaatt tcacgtggca acgttatacc
     7201 ctcatctatg gcatccacga ttacttttat tgtatccctg gataagtatt tggccatttg
     7261 ctttcgatta aaaacgtact tgccataata atccgacggt tgccccttgg gagcttcctc
     7321 catcacggga aggcgctttg atgcttccac aattgcactg aatctcaatg ttgacataat
     7381 gaattttatt ttaaatgttt agcttcaaat atccacaaat aatcaatata taatatcttt
     7441 ttgatttttt atcgacgcaa agataaacac tttgattttt acacacaaga aaaacagcaa
     7501 atttctttta ttttatttaa aacatatcat tttgacaacc atgcc
//
LOCUS       DAOSDR010000119         7517 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrimonas sp. isolate AMR_MDS_1826
            NODE_3594_length_7517_cov_3.848566, whole genome shotgun sequence.
ACCESSION   DAOSDR010000119 DAOSDR010000000
VERSION     DAOSDR010000119.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863355
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrimonas sp. (wastewater metagenome)
  ORGANISM  Petrimonas sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Dysgonomonadaceae; Petrimonas.
REFERENCE   1  (bases 1 to 7517)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7517)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:10:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,720
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 35
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7517
                     /organism="Petrimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3594_length_7517_cov_3.848566"
                     /isolate="AMR_MDS_1826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2023866"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            293..1048
                     /gene="tpiA"
                     /locus_tag="PLI30_06040"
     CDS             293..1048
                     /gene="tpiA"
                     /locus_tag="PLI30_06040"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012457684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HOI79150.1"
                     /translation="MRKKIVAGNWKMNKNLQEGLTLAQELDAALKGKQVNCEVVIGTP
                     FIHLASVANAIDTEKIGVAAQNCADKASGAYTGEVSAEMVASTGAKYVILGHSERRAY
                     YHETVGILKEKVLLALENNLTPIFCIGEVLEEREANRHFDVVKSQITSSLFDLSKEDF
                     SKIVLAYEPVWAIGTGKTATAAQAQEMHAFIRKTLVEKYGEEVAENTSILYGGSCNAS
                     NAKELFSNPDVDGGLIGGASLGVDKFMPIIEAF"
     gene            1124..1642
                     /locus_tag="PLI30_06045"
     CDS             1124..1642
                     /locus_tag="PLI30_06045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006801633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HOI79151.1"
                     /translation="MKFSYNLLLLPLLLVFCLNATAQSQKQHKEIIADLNAVKPGQGR
                     VMVFEDKAIAEVLGRSMAPARTVYATGDGSVQYVKMRGFKIQAFSGNNQRTSKNEAYY
                     KQGLINTSFPGQETVVTFDSPFWRLRVGNFKTREDATAVLNDMRKTFPAFGKEMYIVI
                     DEVKIPVDQLQE"
     gene            1651..2223
                     /gene="folE"
                     /locus_tag="PLI30_06050"
     CDS             1651..2223
                     /gene="folE"
                     /locus_tag="PLI30_06050"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019539926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_process: GO:0042559 - pteridine-containing compound
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HOI79152.1"
                     /translation="MSPEETEDRKSIIADHMKEVITLLGEDVARDGLIKTPDRVAKAM
                     QHLTRGYYQDPEEILRSALFRENYRQMVIVKDIDLFSLCEHHLLPFFGKAHVAYIPDG
                     YITGLSKIARVVDVFARRLQVQERLTTQIKECIQKTLNPMGVMVVIEAQHMCMQMRGV
                     EKQNSITTTSDFTGVFKEQKTREEFIALIK"
     gene            complement(2236..4038)
                     /gene="typA"
                     /locus_tag="PLI30_06055"
     CDS             complement(2236..4038)
                     /gene="typA"
                     /locus_tag="PLI30_06055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019539026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HOI79153.1"
                     /translation="MQKIRNIAIIAHVDHGKTTLVDKMLLAGNLFRENQHTEDLILDS
                     NDLERERGITILSKNVSIQYKDVKINIIDTPGHADFGGEVERVLNMADGCLLVVDAFE
                     GPMPQTRFVLQKALEIGLKPILVINKIDKPNCRPEEVQENVFDLMFSLDATEDQLDFP
                     TVYGSAKQGWMSDDWKKPSTNIIPLLDAIVKYIPAPKSREGTPQMLITSLDYSNYVGR
                     IAVGRVHRGTLEPNKDYALCKRDGSINKIRIKEVDVFEGLGRVKTDAVLSGDICALIG
                     IDGFDIGDTITNLEKPEPLDPIAIDEPTMSMLFTINNSPFFGQDGKYVTSRHIYERLM
                     RELDKNLALRVEETGSSDSWTVFGRGVLHLSVLIETMRREGYELQVGQPQVIIKEIAG
                     EKYEPVEQLTVNLPEESASKIIDIISRRKGEMKSMESKNDRMHIEFTIPSRGIIGLNN
                     IVLTLSAGEAIMAHRFLEFQPWKGNIEKRQNGSIIAGESGNAFAYALDKLQDRGHFFI
                     EPQTDVYEGQVVGEHSKEGDLVVNVTKSKKLTNVRASGTDDKAQLAPPIIFSLEEALE
                     YIKEDEYVEVTPKAIRIRKILLDENDRKRVAKKV"
     gene            4335..4697
                     /locus_tag="PLI30_06060"
     CDS             4335..4697
                     /locus_tag="PLI30_06060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005939270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HOI79154.1"
                     /translation="MMRLTEKEEEVMQILWDKGPLFVREILEVYPEPKPHYNTVSTVI
                     RVLEEKNFVSHKAYGNTYQYFPVVSQDEYKGRALKKVISQYFDDSYTHVVSTLIEEER
                     LSISELKDLIRKIERGKK"
     gene            4709..6799
                     /locus_tag="PLI30_06065"
     CDS             4709..6799
                     /locus_tag="PLI30_06065"
                     /inference="COORDINATES: protein motif:HMM:NF017390.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M56 family metallopeptidase"
                     /protein_id="HOI79155.1"
                     /translation="MENLMVYLAKATICLIAFSVFFRLLLMRETFFRFTRFTLISGLI
                     VCTILPFVKLKLSQSHALQQTVFQFEELLLPEKLKSPEARYVLPEDERVERANPEAAV
                     SQHTTGVSIGRPISPVTALTVIYWLGVGIMLLRLGASFISLYRLLGKSRRIEKEGYKL
                     IVSSGSIVPFSFFRYIVLSEKDYLENPDEIILHEKMHIHQKHNIDVFFSELFLAVHWF
                     NPMVWLLCRDLREIHEYEADNAVIEAGIEAQKYQLLLVKKAVGERRFTSVVNGFNQSK
                     IKNRIAMMLKTESSRWARLKVLVAVPLVVVALLAFSQPESVKDQFVSYLERKAAVDYF
                     LSVRRVQKENYLAYLYLDAKGQLFLMSENADTALIQVADVNERMDLVEAFSGLITGKL
                     NENTPAPVDFILGAENDTPMRSVSLVKEAVREVYGRSCGAVSKEKNASPKEVAEKFPL
                     SITFTSLQANDPEGIVQQVQSNPYFYWEQVWKYCKEKGIEPKDIDFRAADNRNLILVL
                     INSVNAVMYTNYGGSEWFKTQEEGMSATSVEVLKKMIVETMKKNSDNPFYISLQHDKA
                     SSTAFIITFIREVLPRAYEEALKEVSIRKGVSFDELGESKPLLLFYAVPRVFGAPAKV
                     GNDRVSEKGARFQVRTTTRKHDTEEMMFIVENGLVIVDEKMDQSDVTGIQKMLSKEDK
                     LAAKKTYFVLGTGF"
     gene            complement(6772..7389)
                     /locus_tag="PLI30_06070"
     CDS             complement(6772..7389)
                     /locus_tag="PLI30_06070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009037035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate mutase family protein"
                     /protein_id="HOI79156.1"
                     /translation="MDILTLAAENQQTAWQILKETGIVQTWEKAGATVNLVGSLKSGL
                     LMKNRDIDMHIYTDKLSVSESFSVIQELAERLSLKEIHYKNLIDTEEECIEWHVLYEH
                     NNRDTWKFDMIHIRKGSRYDGTVERVTDAITECLTPEIRKKILQLKQEIPDSITIPGI
                     EIYHAVFEGDVSSYEELEQWRKNHPLINSLDWLPQDLQNPVPRTK"
BASE COUNT         1994 a         1528 c         1851 g         2144 t
ORIGIN      
        1 agttgctgaa agccaaggga tggaaaatga acttttgggg taaactgatg gttatactcc
       61 tgatatttat tgttccgctt ttactggtaa aactggaaga atttaagaga aagcggtcgg
      121 ggctcccggc tataaaaaag ggctatacta aaaaaatata gtttcatata tattcgtagc
      181 taatttatat agctaaaaat gaggtatgta agtcgagggt ttagctccca atgcgatgat
      241 ttggaaatta aataaacaat acgcacattt atatattata aaaacaacaa ttatgagaaa
      301 aaagattgta gcaggcaact ggaaaatgaa caagaacctg caggaagggt tgacattggc
      361 acaagagctg gatgcggcct taaaagggaa acaggtgaat tgcgaggtgg ttatcggaac
      421 tccgtttatt catctggcaa gcgtggctaa tgccattgat acggaaaaaa ttggcgtggc
      481 agcgcaaaac tgtgccgaca aggcatcggg agcatacacc ggtgaggttt cggctgagat
      541 ggtggccagc acgggagcga aatacgtgat tttagggcac agcgaacgcc gggcatatta
      601 ccacgagaca gtaggtatac tgaaagagaa agtattgtta gcgctggaaa acaaccttac
      661 ccccattttc tgtatcggag aagtgttgga agaacgtgaa gccaaccgtc atttcgatgt
      721 ggttaaatcg cagattacat cgtcgttgtt cgacttgtcg aaagaggatt tctccaagat
      781 cgtactggct tatgaacctg tttgggccat cggtaccgga aagacagcaa cggctgccca
      841 ggcacaggaa atgcatgcgt ttatccgcaa aactctggtc gaaaagtatg gggaagaggt
      901 ggctgaaaat acgtccatcc tttacggagg aagctgtaac gcgtcaaatg cgaaagaatt
      961 attttccaat cccgatgttg acggcgggtt aattggtgga gcatccttgg gggttgataa
     1021 gttcatgccc attatagagg ctttttaatg cattaaagaa ttttaccaca gatttgaact
     1081 caggtctgtg gtatttctct atctttgcag aaattttttc actatgaagt tttcatacaa
     1141 cctattgctt ttgccgttgt tgctggtatt ttgtcttaac gcaacagcac aatcgcaaaa
     1201 acaacacaaa gagatcatag cggacctgaa tgccgtaaaa cccggtcagg gccgggtgat
     1261 ggtttttgag gataaagcca ttgcggaggt gttgggacgg agtatggcac cggcgagaac
     1321 ggtttacgct accggtgacg gatcggttca gtatgtgaaa atgcggggat ttaagattca
     1381 ggcattctcc ggaaacaacc agcggacttc aaaaaatgag gcatattata aacaggggct
     1441 cataaatacg tcctttcccg gacaggagac cgttgttact tttgattctc ctttctggcg
     1501 gctgcgagtt ggaaacttta aaacccgcga agacgctacc gcggttttga acgatatgcg
     1561 gaagactttt cctgctttcg gcaaggagat gtacattgtc atagatgagg tgaaaatacc
     1621 tgtcgatcaa cttcaagaat aatttagccc atgtcgccgg aagaaacaga ggacagaaag
     1681 tcaatcatag cggatcacat gaaagaggtg atcaccctgc tgggtgaaga cgtagcccgt
     1741 gatgggctga tcaagacgcc cgaccgggta gccaaagcga tgcaacacct gacaaggggg
     1801 tattaccagg accccgaaga gatattgcgt tctgcactgt tccgggagaa ttaccggcag
     1861 atggttatcg taaaggatat cgacctgttc tcgttatgtg aacatcacct gttgccgttt
     1921 ttcgggaaag cccatgtggc atatatccct gacggataca ttacaggatt aagtaaaatt
     1981 gcccgggtgg tggatgtgtt tgcccggcgt ttgcaggttc aggaacggct gaccacccag
     2041 atcaaggagt gtattcagaa aacacttaac ccgatgggcg tgatggtggt gattgaagcc
     2101 caacatatgt gtatgcagat gcgtggtgtt gaaaaacaaa attccatcac cactacttcg
     2161 gattttaccg gagtttttaa agagcaaaaa acccgggaag agtttattgc cctgatcaaa
     2221 taagcggaga cgcttctaaa ctttctttgc cacccttttc cggtcgtttt cgtccaaaag
     2281 gatctttcgt atccggatgg ctttgggcgt aacttccacg tactcatctt ccttgatgta
     2341 ttccagtgct tcctcgaggc tgaaaattat cggcggagcc agttgggctt tgtcatcggt
     2401 tcccgatgcg cgtacgttcg tcagtttttt cgatttggtg acgtttacca ctaaatcgcc
     2461 ttctttgctg tgctcgccga ccacctgtcc ttcgtacaca tcggtctggg gttcgatgaa
     2521 gaaatgtccc cggtcctgga gcttgtccag tgcataggca aaggcattgc ccgattctcc
     2581 ggcaataatg gatccgttct ggcgcttttc tatgttgcct ttccagggtt ggaattccag
     2641 aaaccggtgt gccatgatgg cttccccggc agagagcgtg agaacgatat tgtttaatcc
     2701 gataatgccg cgcgagggga tggtaaactc aatgtgcatg cggtcgttct tgctttccat
     2761 gcttttcatt tcgcctttcc gccgggagat aatatcgatg attttgcttg ccgattcttc
     2821 gggaaggttt acagtgagtt gttctaccgg ttcgtatttt tccccggcga tctctttgat
     2881 gatcacctgg ggctgcccca cctgtagttc atagccttct cgccgcatgg tctcgatcag
     2941 tacagacaga tggaggacac cccgtccgaa caccgtccat gaatcggagc tgccggtttc
     3001 ttccacacgc aacgctaagt ttttatccaa ctcacgcatc agtctttcgt aaatgtggcg
     3061 tgacgtcacg tacttgccgt cctgtccgaa aaaaggagaa ttgttgatgg taaagagcat
     3121 cgacatggtg ggctcgtcga tagcaatcgg atcgagcggt tccggtttct ccaggttggt
     3181 gatcgtatcg ccgatatcaa acccatcgat accgatcaac gcgcaaatat cacccgacaa
     3241 aacagcatcg gttttgaccc ttcccaatcc ctcgaacaca tctacttcct ttatccggat
     3301 cttgttgata ctgccatcgc gtttacaaag cgcataatcc ttgttgggtt caagagtgcc
     3361 ccggtgtacc cggcccactg cgatcctgcc cacgtaattg gaataatcga gtgaggtgat
     3421 gagcatttgc ggagtccctt ctcgcgattt gggagcgggg atatatttga cgatggcgtc
     3481 gagcagggga ataatgttgg ttgacggttt tttccaatcg tcggacatcc atccctgttt
     3541 ggccgagccg taaacagtag ggaagtcgag ctggtcttcg gtagcatcaa ggctgaacat
     3601 caggtcgaaa acgttttcct gtacctcttc ggggcgacag ttaggcttgt cgatcttgtt
     3661 gataaccagg atcggtttta atcctatctc taacgctttt tgcaaaacaa aacgggtttg
     3721 gggcatgggg ccttcaaaag catctacaac cagcaggcag ccgtcggcca tgttcagcac
     3781 cctttccact tcaccgccaa aatcggcgtg tccgggtgta tcgataatgt ttatttttac
     3841 gtctttgtat tgaatggaaa catttttgga taaaatggtg atgccccgct cacgttccag
     3901 gtcgttgcta tcaagtatta aatcttcggt gtgctgattt tccctgaaca ggtttccggc
     3961 caggagcatt ttgtccacca gggtagtttt accatgatca acgtgggcga taatggctat
     4021 attgcgtatt ttttgcataa aacaatcttt ttctgggatg caaaggtacg ttttttcatt
     4081 gaaaaatttg taagttattg aaaaactgtt ttaaattttt tggttgatcc gcaaaagtta
     4141 tggatgctca gaaaaacagt tccggttatt gagcatattc aagggcgtaa atgatatcta
     4201 atttttcttt aaccgtttac aactattaaa acagtatata aaactgttat atatagttat
     4261 attattaatt tttatagttg tttttttagg aagtatgttt acatttgtgt gagaatcgta
     4321 taaaacaaag ccttatgatg agattaaccg aaaaagaaga agaggtgatg caaattttat
     4381 gggataaagg acctttgttt gtccgcgaaa tccttgaggt ttatccggaa cctaaacctc
     4441 attacaatac cgtttctacc gttatccggg tgcttgaaga aaagaatttt gtaagccata
     4501 aagcgtatgg aaatacgtat cagtattttc ccgttgtgtc gcaggatgag tataaaggca
     4561 gggcactgaa gaaagtgatt tcccaatact ttgatgactc ttatacccat gtggtctcta
     4621 cattgatcga agaggagcgg ttgtcgatca gtgaattgaa agacctgata aggaaaattg
     4681 aaagaggtaa aaaataattc ttccgaagat ggaaaacctt atggtatacc tggccaaggc
     4741 aaccatttgc ctgattgctt tctccgtatt cttccggttg ttgttgatga gagagacttt
     4801 ctttcgtttt acgcgtttca cgttgatttc aggattaatc gtctgcacaa tcttaccttt
     4861 tgtaaaactg aaactgagtc agtcgcatgc tcttcagcaa actgtttttc agtttgaaga
     4921 gttgctgtta ccggaaaaac ttaaaagccc tgaggctcga tacgtattgc cggaagatga
     4981 gcgggttgag cgtgccaacc ctgaagcggc tgtttcgcag cacacgaccg gtgtaagcat
     5041 tggaaggccg atctccccgg ttacggcatt gactgtgatt tattggctgg gtgtaggaat
     5101 aatgctgttg cgcttgggag cctcttttat cagtttgtat cgtctgcttg gaaagagcag
     5161 aaggatcgag aaagaaggct ataaattgat tgtcagttcc gggagtatcg ttccgtttag
     5221 ttttttccgt tatatcgtat tgtccgagaa agattatctg gagaatcccg acgaaattat
     5281 cctgcacgaa aaaatgcata tccaccagaa acacaatatc gatgtctttt tttcggaact
     5341 ttttcttgct gtccactggt ttaatccaat ggtctggctg ttgtgccgcg atttacgcga
     5401 gatccatgaa tacgaagccg ataatgcggt gattgaagcc ggcattgaag ctcaaaagta
     5461 tcaattgtta ctggtaaaaa aagccgttgg cgaaaggcgc ttcacttctg tagtaaacgg
     5521 gtttaatcaa tcaaaaatta aaaacaggat tgctatgatg ttaaaaacag aatcatcgag
     5581 atgggcgcgg ctcaaagtgt tggttgccgt gccgttggtt gtcgttgctt tattggcgtt
     5641 ttcacagcct gaaagtgtga aagatcagtt tgtaagttat ctggaacgaa aagcggccgt
     5701 ggattatttt ctttcggtga gaagggtgca gaaagaaaat tacctggctt acctgtatct
     5761 ggatgcaaag gggcagttgt tcctgatgag tgaaaatgcg gatactgctt tgatacaagt
     5821 agctgatgtg aacgaaagga tggatttagt agaagctttc agcgggctaa tcaccgggaa
     5881 gcttaatgaa aacaccccgg cacctgtcga ttttattctg ggagccgaaa atgatacccc
     5941 tatgcggagt gtttcactgg tgaaagaggc tgtgcgtgag gtgtacggca gatcgtgcgg
     6001 agctgtttcg aaggaaaaga atgcttctcc gaaagaagta gcggaaaagt ttcccttgtc
     6061 gattactttt acttctcttc aggcgaatga tcctgaagga attgtacaac aggtgcagtc
     6121 taatccctat ttttattggg agcaggtgtg gaagtattgt aaagagaaag gtattgaacc
     6181 gaaagatatt gatttcaggg cggctgacaa tcgaaacctg atccttgttt taattaattc
     6241 ggtcaatgcg gtaatgtata caaattacgg cggttccgag tggtttaaaa cccaagagga
     6301 ggggatgagc gctacgtcgg tcgaggtgtt gaagaagatg attgtggaga cgatgaaaaa
     6361 aaacagcgac aacccttttt atatctcgct gcagcatgat aaggcctctt caaccgcttt
     6421 tatcatcacg tttatacggg aggttttgcc ccgggcttac gaagaagcgt tgaaggaagt
     6481 ctctatccga aaaggtgttt ctttcgacga acttggggag agcaaaccgt tgttgctttt
     6541 ttatgccgta ccgcgggttt ttggtgcccc ggcaaaagtc ggaaacgacc gggtaagcga
     6601 aaaaggggcc aggtttcagg tgcgtaccac cacgaggaag cacgatacgg aagagatgat
     6661 gtttattgta gaaaatggtc tggtcatcgt tgatgaaaaa atggaccaat cggatgtgac
     6721 cggtattcag aagatgttga gcaaagagga taagctggcc gctaaaaaaa cctactttgt
     6781 ccttggaacg ggattttgaa gatcctgtgg aagccagtcc aggctgttta tcaacggatg
     6841 gttcttcctc cactgttcaa gttcttcata actgcttaca tctccctcaa aaacagcgtg
     6901 ataaatttct attccgggaa tggtgatgct gtccggaatc tcctgtttca gttgaaggat
     6961 tttcttgcgg atttctggtg tcaggcattc ggtaatggca tctgtaactc tctctaccgt
     7021 accgtcatat cttgatccct ttcggatatg aatcatgtca aatttccagg tgtcccggtt
     7081 gttgtgttca tataaaacgt gccactcgat acattcctct tcggtgtcaa tcaggttttt
     7141 ataatgaatt tccttcaaag ataacctttc tgccaattcc tggataacgg agaaactttc
     7201 ggatacactg agtttgtccg tgtagatgtg catatcaata tccctgtttt tcatcaacag
     7261 gcccgacttg agtgagccta ccaggttcac ggtcgctcct gccttttccc aggtttgaac
     7321 aatccctgtc tctttcagta tttgccaggc cgtttgttga ttctctgcgg caagtgtaag
     7381 tatatccata cagctgattt tatcgggaca aagatacgca tatgcttttt gtcaaccggg
     7441 tttgttgaaa aaaattgtac atttggtttt ttaaactgaa aacgtatgac accttccgaa
     7501 ctgctccata tccgcct
//
LOCUS       DAOSDR010000120         7512 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrimonas sp. isolate AMR_MDS_1826
            NODE_3601_length_7512_cov_3.681507, whole genome shotgun sequence.
ACCESSION   DAOSDR010000120 DAOSDR010000000
VERSION     DAOSDR010000120.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863355
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrimonas sp. (wastewater metagenome)
  ORGANISM  Petrimonas sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Dysgonomonadaceae; Petrimonas.
REFERENCE   1  (bases 1 to 7512)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7512)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:10:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,720
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 35
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7512
                     /organism="Petrimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3601_length_7512_cov_3.681507"
                     /isolate="AMR_MDS_1826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2023866"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..765)
                     /locus_tag="PLI30_06075"
     CDS             complement(<1..765)
                     /locus_tag="PLI30_06075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008779027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 92 protein"
                     /protein_id="HOI79157.1"
                     /translation="MLKKNLIKANTVAILFALITGCSDRVQQPADYVNPFIGASTNME
                     KAGASHGLGKTFPGAATPFGLTQLSPNTITGGDNASGYSNEHTTIEGFAFTQMSGTGW
                     YGDLGNFLVMPVTGELKTAAGKEEKPEEGYRSRYSKEDETASAGYYGVTLTDYGIRAE
                     MTAAPHSGIMRFTFPEHNQSRIQVDLARRVGGTSTHQFIQVMDDHTIRGWMKCTPEGG
                     GWGNGDGKADYTVYFYAQFSKPFINTGVWSAEIPDSC"
     gene            complement(817..2313)
                     /locus_tag="PLI30_06080"
     CDS             complement(817..2313)
                     /locus_tag="PLI30_06080"
                     /inference="COORDINATES: protein motif:HMM:TIGR00196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HOI79158.1"
                     /translation="MKILTAEQIREIDLKTTTYENISSLELMKRASKAFFDWFTTRFT
                     DKNLPVSVFSGTGNNGGDGLVVARMLQKSGYKVNVFIVEYSQHYSEDCAHNVRRAKVE
                     NIPIKKITSTGDLPSLSGFEIIIDAIFGTGLNREVTGIAREVIEKINRSGKKIISIDV
                     PSGLSMNGKTYFAVHATETVTMQIPKLALYLPDNRNFTGEVYLINIGLNETAISDAKT
                     AFYFITKQEIKELLKPLDKFAHKGTQGHSLLIGGSIGKMGSVCLASRAALKTGCGLVT
                     AYVPKCGTSVLQSAFPEAMVVEDRNDTHITGIDFTIHPDAVGAGVGLSESPETQHAFY
                     RFLQQNKSPLVIDADGLNILSKNKEWLALLPSRTILTPHPKELFRLIGVWNEDYDKIY
                     KTREFAKKYRLIIVLKGAYTLIIDSENVYVNSSGTPALATAGSGDVLTGIITSLLAQG
                     YEPLDAAKAGVFIHGLTANLSATKIHARSFTASDIIDNIGNAYFDIEK"
     gene            complement(2401..2484)
                     /locus_tag="PLI30_06085"
     tRNA            complement(2401..2484)
                     /locus_tag="PLI30_06085"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2448..2450),aa:Leu,seq:gag)
     gene            complement(2501..2584)
                     /locus_tag="PLI30_06090"
     tRNA            complement(2501..2584)
                     /locus_tag="PLI30_06090"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2548..2550),aa:Leu,seq:cag)
     gene            complement(2686..3957)
                     /locus_tag="PLI30_06095"
     CDS             complement(2686..3957)
                     /locus_tag="PLI30_06095"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOI79159.1"
                     /translation="MNRRNFIRNVSALSTLTILKPEIVFASNTNSAVRIGLIGCGSRA
                     TGILGSMADNANIHITALADIFEDKVIKGIEFANGLNKKRNYAAVAKNKTYVGSDAYL
                     RLLEDKEVDAVIIASPGYSHPQILEDTVAAGKHVYCEKPLAVDADGCNQIIRTANTIN
                     GKISAFDGFQIRYATPYVEMAKRIKRGDIGELVTVQLYYFSSGAAIIPYEGMSYDEMR
                     IRNHYHFHEISGGCYLDQAIHMIDVCNWILGTTPLYAIGDGSKKGGPDFGNAWTNYQV
                     IYKYPNDVNVSVHSSKFGKVFGDVCARFIGTKGLAEAHYSGGVFINGDNKWDSGIVRS
                     ASELTPESIAKGASSSSLDDADPNKGKAFIESITSGNYLNQLESGCHSTLSAILGREA
                     ASKQEKIMWDELIYTPQKIDPKLDLKQFPRK"
     gene            complement(3982..5052)
                     /locus_tag="PLI30_06100"
     CDS             complement(3982..5052)
                     /locus_tag="PLI30_06100"
                     /inference="COORDINATES: protein motif:HMM:NF014826.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HOI79160.1"
                     /translation="MKKVSVFLMLCSVLSLQAQLRNNDVYEVTEMDNSRVRSEIHIPD
                     IDGYKTLKCDFHIHTVFSDGKVWPDIRVAEAWQEGLDAIAITDHIEYRPNKETLKGDL
                     NESYKIAKRAGDATGFMVIHGTEITRSKPLGHLNALFISDAMPMDVEDPLEAIEKALQ
                     QGAFIMWNHPGWPDDKSTLYPVHQELIKAGKIHGVEVFNYMEYYPVTFDWSKNNNLAF
                     MANTDIHGLINTDYGAGNKIRPMTLVLAKERSVESIKEALFAKRSIAYFHGELAGKQE
                     HLKALLKASVKIRIINEKRAEVTNISDITYRAVSGNNLYILPANKTVLLTIPGPETVF
                     TVENCHTGNGEKLTVTVSDFDI"
     gene            complement(5167..5239)
                     /locus_tag="PLI30_06105"
     tRNA            complement(5167..5239)
                     /locus_tag="PLI30_06105"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5204..5206),aa:Gly,seq:gcc)
     gene            complement(5329..5805)
                     /locus_tag="PLI30_06110"
     CDS             complement(5329..5805)
                     /locus_tag="PLI30_06110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008768394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0070191 - methionine-R-sulfoxide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HOI79161.1"
                     /translation="MAEEIIIPEGISKAEKYETLILQLKPLVESETDVIANMANISAA
                     LKEIFRFFWVGFYIVRDEQLVLGPFQGPVACTRIRYGKGVCGTAWKEQKPILVPNVDE
                     FPGHIACSSLSRSEIVVPVFHYGKITAVLDVDSEFLNHFDETDEKFLSEIAGWIKF"
     gene            complement(5875..6555)
                     /locus_tag="PLI30_06115"
     CDS             complement(5875..6555)
                     /locus_tag="PLI30_06115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019539695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HOI79162.1"
                     /translation="MKQIFKFFGIIILGGALVLSGCGTSNTVKGTGIGAGAGAAVGAG
                     VGAIAGGGKGAAWGAGIGAVLGGAAGAIIGNKMDKQAAELEQIEGAQVEKINEGEAIK
                     VTFESGILFATNSSTLNTASRASLDKFATSLLNNPDTDVKIYGHTDSTGSDAINNPLS
                     DRRAESVYNYLVSKGVAGTRMTSQGYGSSQPVADNSTVAGRAQNRRVEVFILPNAKMV
                     KEAQEQVK"
     gene            complement(6602..6925)
                     /locus_tag="PLI30_06120"
     CDS             complement(6602..6925)
                     /locus_tag="PLI30_06120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005635220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI79163.1"
                     /translation="MSKEKTIIEYDEDESVKFIRKSLPEEMQNEFSDDEINYIVDLVY
                     EFYEEKGFLDEDDDKDIEIDEDELLDYVIKNARKDKIRDFTDEQVEAIVAGELAYCDT
                     LNLFE"
BASE COUNT         1955 a         1687 c         1533 g         2337 t
ORIGIN      
        1 acacgaatcc gggatttcgg cactccaaac ccccgtattg ataaaaggct tactgaattg
       61 ggcataaaag tagacggtat aatccgcttt tccgtcaccg tttccccatc cgcctccttc
      121 cggagtgcat ttcatccatc cccggattgt atggtcgtcc atcacctgga taaactggtg
      181 agttgatgtg ccgccgactc ttcgcgcaag gtcaacctga atacgtgatt ggttatgctc
      241 tggaaacgta aagcgcatga tcccactatg aggagcggca gtcatttcag cgcgaatacc
      301 ataatctgtc aacgttacgc cgtaataccc ggcagatgct gtttcgtctt cttttgagta
      361 gcgggagcgg tatccttctt ccggtttttc ttcttttccg gctgctgttt ttaattcacc
      421 ggttacgggc ataaccagaa aatttcccag gtcgccatac catccggtac cgctcatttg
      481 ggtaaaggca aatccttcaa tggtcgtatg ttcattgctg taaccggaag cattgtctcc
      541 tcctgtgatt gtattcggac tgagttgagt cagtccaaaa ggagtagcgg cgccggggaa
      601 tgtttttccc agtccgtgac tggcaccggc tttctccatg ttggtactgg caccaataaa
      661 ggggttcacg taatcggcag gttgttgaac acggtcggag caacctgtta ttaaagcaaa
      721 taaaatagcc acggtgttgg ctttaatcaa attcttttta agcatgttgt ttgacttagt
      781 ttatttttct tatcaacagt acaatgtttg aaattgctat ttctcgatgt caaaataggc
      841 atttccgatg ttatcgataa tgtccgaagc tgtaaaactt cgggcgtgaa tcttcgttgc
      901 cgacaagttt gccgttaaac cgtggataaa aacacctgct ttagctgcgt ccaaaggctc
      961 ataaccttgt gcgagcaagc ttgtgatgat acctgtcagt acatccccgc ttccggctgt
     1021 agccaacgcc ggggttccgg aactgttgac ataaacattt tctgaatcga taatcagcgt
     1081 gtaagctcct ttcagaacga taattaaccg gtattttttt gcgaattccc ttgttttata
     1141 tatcttatca tagtcttcgt tccacacccc aataagtctg aaaagttctt ttggatgagg
     1201 ggtcaagatt gttcttgagg gtaatagggc taaccattct ttattcttcg acaaaatatt
     1261 caatccgtca gcatcgataa ccagagggga tttattctgc tgcaaaaagc ggtaaaaagc
     1321 atgttgtgtt tccggtgatt cgcttaagcc cacaccggcc ccgacggcat ccggatgtat
     1381 ggtgaaatcg attcccgtaa tgtgtgtgtc gtttctgtct tcaaccacca tcgcttcggg
     1441 aaaagcggat tgaagaaccg atgttccaca tttgggtaca tatgcagtga ccagtccgca
     1501 tcctgttttc aaggccgccc gggatgccag gcagactgag cccatcttcc cgatgcttcc
     1561 gccgataagc aaactgtgtc cttgagtacc tttatgagca aatttatcca acggtttcag
     1621 cagttctttt atttcttgtt ttgtgataaa gtaaaaagcg gttttcgcat cactgatggc
     1681 cgtttcgttt agtccaatgt ttatcagata cacctctccg gtgaaatttc ggttgtccgg
     1741 caaatacaga gcaagcttgg ggatctgcat cgttaccgtt tctgtggcgt gaacggcaaa
     1801 ataagttttt ccgttcatcg acaatccgct aggtacgtcg atgctgatga tttttttccc
     1861 gctcctgttg attttttcaa tcacttcacg ggctatgcct gtcacttccc gattaagccc
     1921 tgttccaaaa atagcatcaa taattatctc aaagccggac aaggaaggga gatcaccggt
     1981 tgaggtaatt tttttaatcg gaatattttc aactttggca cgacgaacat tgtgcgcgca
     2041 atcttcggaa tagtgctgac tgtactctac tataaaaaca ttaaccttgt atcccgattt
     2101 ctgaagcatt cttgcgacaa ccaatccgtc accgccgtta tttcccgttc cagaaaatac
     2161 cgaaacagga aggtttttat cggtaaacct cgttgtaaac cagtcgaaga aagcctttga
     2221 tgctcttttc atcagttcca gcgaagaaat gttttcgtag gtagttgtct ttaagtcaat
     2281 ctccctgatt tgttcggcag tgagtatttt cattttcatc ggttttagca cgctaagtta
     2341 tcttttttcc ctgtaaaaac aaaaacaggc atccaatttc ttgaatacct gttttagttg
     2401 tgcgcagaat gagactcgaa ctcacacgcc gtaaccggca ctaccccctc aaagtagcgt
     2461 gtataccaat ttcaccacct gcgcaaacat gtttgcgaag tgcccggaac aggactcgaa
     2521 cctgcacatc gtttccaaca ctagtccctg aaactagcgc gtctaccaat tccgccatcc
     2581 gggcgtgctc cactacttat agaaatatac ggccactgcc gtgttcattt tttgtggctg
     2641 caaagttaaa tcttttttaa acctttacaa aatatttttc aggttttatt ttctcggaaa
     2701 ctgttttaaa tccaattttg ggtcaatttt ctgaggagtg taaatcaatt cgtcccacat
     2761 tatcttttct tgtttcgaag cagcctctct tcccaggatc gcactcaacg ttgagtggca
     2821 gcccgattcc agttggttta aataattccc gctggtaatg ctttcgatga aagctttgcc
     2881 tttgttgggg tcggcatcgt ccagtgaaga tgaactggca ccttttgcta tggactccgg
     2941 agtcagttcc gatgcagaac gcacaattcc cgaatcccat ttgttgtcgc cgttgataaa
     3001 gactcctccg ctgtaatgtg cttctgccaa ccctttggtc ccgatgaatc gggcgcatac
     3061 atcaccaaaa acttttccga acttggaaga atgaacagat acgtttacat cgttgggata
     3121 tttgtatatt acctggtagt tggtccaggc atttccaaaa tctggtccgc cctttttgct
     3181 gccgtctcca attgcgtata gcggagtggt accgagaatc cagttgcaaa catcgatcat
     3241 atgaatggcc tggtcgaggt aacatcctcc cgaaatttcg tggaaatgat agtggttccg
     3301 gatacgcatc tcatcgtatg acattccttc gtaaggtatt atggcagcac ctgatgagaa
     3361 atagtaaagt tgtactgtaa caagttcacc tatatcgcct ctttttattc tttttgccat
     3421 ttcaacgtag ggtgttgcgt aacggatctg aaaaccgtcg aaagccgaaa tctttccgtt
     3481 aatggtattg gctgtccgga taatctggtt gcatccgtcc gcatcaaccg ctaacggttt
     3541 ttcgcaataa acatgttttc cggcagctac ggtatcttcc agtatttgag gatgtgagta
     3601 accgggagaa gcaatgatga cagcatctac ctctttatct tccaataacc tgagataagc
     3661 gtctgatcca acatatgttt tgttcttagc tactgccgca tagtttcttt ttttgttcag
     3721 tccattagca aactctattc ctttgatgac tttatcttca aagatatcgg caagggcagt
     3781 gatatgaatg ttggcattgt cggccatgct gcccaatatt cccgttgcgc gagacccaca
     3841 cccaatcagc ccgatcctca cggcagaatt ggtattggat gcaaaaacaa tttcgggctt
     3901 taaaatggtc agcgtagaga gtgcagatac atttcttata aaatttcgtc tgttcatatg
     3961 gcatacatat ttaaaatttc actaaatatc gaaatccgaa acggtgacgg ttaatttttc
     4021 tccgttcccg gtatgacaat tctctacggt aaaaacggtt tcaggtccag gaatagttag
     4081 taagacggtt ttatttgctg ggagaatgta taaattattg ccggaaactg cccggtaagt
     4141 aatgtcggag atattagtta cttctgctcg tttttcattg atgatacgta ttttcacgga
     4201 ggctttcagc aaggctttca ggtgttcctg tttacctgcc agctcaccgt gaaaataagc
     4261 gatacttcgt tttgcaaaca gagcttcctt gatgctttcc acacttcgct ctttggccag
     4321 aacgagtgtc atgggacgga ttttgttccc cgcaccgtag tcggtattta tcaaaccatg
     4381 aatatctgta ttggccataa aagccagatt attatttttg ctccagtcaa aagttaccgg
     4441 ataatattcc atgtaattaa atacttctac accgtgaatt tttccggctt ttatcagttc
     4501 ttggtgaacg gggtaaaggg ttgatttgtc atcgggccag ccggggtgat tccacataat
     4561 gaacgcacct tgttgtaaag ctttctcaat agcctccagg ggatcttcaa catccatggg
     4621 catggcatca ctgatgaaaa gtgcgttgag atgacccagg ggtttgcttc gggtgatttc
     4681 tgttccgtga ataaccataa atccggtggc atctccggct cttttggcaa ttttatacga
     4741 ttcgttcagg tctcccttga gggtttcttt gttcggccga tactcaatgt gatcggttat
     4801 ggcaatggca tccaatcctt cctgccaggc ttctgctacc cggatatcgg gccagacctt
     4861 tccatctgaa aaaacggtgt gaatgtgaaa atcgcatttc aatgttttat aaccgtcaat
     4921 gtcaggaatg tgaatttcgc ttctcacgcg cgaattatcc atctccgtta cctcgtaaac
     4981 atcgttgttg cgtagttgtg cttgaaggga caaaacgctg cataacatta aaaaaacgga
     5041 aacctttttc atgttgcttt aaatttagta ctatcggtat aggttttaat tgagtttaga
     5101 aagcccggtt gttaagcaat atacgctcca aaacgatggt ttttggagcg tatatactaa
     5161 catttgagcg gaaaacgaga ctcgaactcg cgaccctaac cttggcaagg ttatgctcta
     5221 ccaactgagc tatttccgca aaggggttta ttttgtaagg attcagcgga tggactgaat
     5281 tcatgttgca aagataattt gtttttttga actttagaaa tttatttctc agaatttgat
     5341 ccatcccgct atttccgaca ggaatttctc atctgtctca tcaaaatggt tgagaaattc
     5401 actgtccaca tccaagaccg cagtaatttt tccgtaatga aaaacgggaa cgacaatttc
     5461 tgatctggac aaagaactac aggcaatgtg tccgggaaat tcatctacat tgggaacaag
     5521 aattggtttt tgctctttcc atgccgtgcc acacactccc tttccgtacc ggatacgcgt
     5581 acaggcaacc ggaccctgaa aaggtcccag taccaactgt tcgtctctga cgatataaaa
     5641 acctacccaa aagaatctga aaatttcttt taaggctgca gaaatgtttg ccatgttggc
     5701 tatcacatct gtttcagact ctaccaatgg ctttaattgc aggattaaag tttcatattt
     5761 ttctgctttt gaaattcctt ccggaataat tatctcttct gccatgcttt tattcaacta
     5821 aaaatcgcac taccgaaacc gataatgcga ttttatatag cctaccttta ttaattattt
     5881 tacttgttcc tgagcttctt ttaccatctt ggcatttgga agaatgaaaa cttctacacg
     5941 gcggttctga gctcttccgg cgactgtact gttgtcggca accggttggg aggatccgta
     6001 accttgagaa gtcatgcgag ttcctgctac acctttggat acgaggtaat tgtaaactga
     6061 ttctgcacga cgatcagaga gcggattgtt gatagcgtca ctacccgtac tgtcggtatg
     6121 cccatagatt ttaacatctg tatcggggtt attcaacaga gatgtggcaa atttgtccag
     6181 ggaagcacgt gatgctgtgt ttaaggtgct tgagttggtt gcaaaaagga ttcctgattc
     6241 gaaagtaacc tttatagcct cgccttcgtt gatcttttca acctgtgctc cttcaatttg
     6301 ttccagctca gctgcctgct tatccatctt gtttccaata atggcaccag cagctccacc
     6361 gagaacagcg ccgattcctg caccccaagc tgcgcctttg cctccgcctg caattgcacc
     6421 tacaccggct cctactgctg cgcctgcacc tgcaccaata cctgtacctt taaccgtgtt
     6481 gcttgttccg caaccggata ataccaaagc accacctaga ataatgatgc caaaaaattt
     6541 aaaaatctgt ttcatagtta atgtttttgg aaaaatatag ttaattgatt tgttaaaaga
     6601 attattcaaa aagattcaaa gtgtcgcaat acgctaattc accggcaacg atagcttcga
     6661 cctgttcatc ggtaaaatcc ctgatcttgt ctttacgggc gtttttaata acatagtcca
     6721 atagctcatc ttcgtcaatc tcaatgtctt tgtcatcatc ttcatccaga aagcccttct
     6781 cttcgtaaaa ctcatatacc aaatcaacga tgtagttgat ttcgtcatcc gaaaattcgt
     6841 tttgcatttc ttcaggcaga cttttccgta taaattttac ggattcatct tcatcgtact
     6901 caataatagt tttctcttta ctcataccga taatatttgt agtttacttt tgattgaatc
     6961 agtagtatta tttttaattt tcaacctcaa tcttaaaaca actattcaaa aaataagttc
     7021 aacaagtact tgggatttta gggaaaactt actctgcaac ctaaaaaaga agaaaatcag
     7081 gaagaaaatt gtaaaaagtc ttacccaaga gaaactgatt gagttcgtaa ggttattggc
     7141 agattcgacg aagattaagc tggagataaa aattgaagat tcaatagtgc taaaatatct
     7201 tgtaacactt gaaaaggatg cggctgaacc agagagtgcc aacaagggca ccttgtaaac
     7261 ctattttaaa caaattgccc aaaggcatgc cggtatgccg ataccggatg aagaaggttc
     7321 ggtttaaaag gaaaacagcc cgagagaaaa attctcccgg gccattttct atcgttgaaa
     7381 ttattgaaaa ttctacgttt acttgcggta atttttcttt gctgcttctt ttgccctttc
     7441 gcgagcttgt ttttcctgca ttttttgagc ttcagccagt cgagccataa aacccgattg
     7501 ttttttgggc tt
//
LOCUS       DAOSDR010000121         7510 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrimonas sp. isolate AMR_MDS_1826
            NODE_3604_length_7510_cov_3.491616, whole genome shotgun sequence.
ACCESSION   DAOSDR010000121 DAOSDR010000000
VERSION     DAOSDR010000121.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863355
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrimonas sp. (wastewater metagenome)
  ORGANISM  Petrimonas sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Dysgonomonadaceae; Petrimonas.
REFERENCE   1  (bases 1 to 7510)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7510)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:10:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,720
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 35
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7510
                     /organism="Petrimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3604_length_7510_cov_3.491616"
                     /isolate="AMR_MDS_1826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2023866"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..215
                     /locus_tag="PLI30_06125"
     CDS             <1..215
                     /locus_tag="PLI30_06125"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="HOI79164.1"
                     /translation="LTLKVSLDDCRSWSNSKLVYEGPAAYSCITRLPNGRVGLFFEAG
                     EKRPYEKMIFVSFDWNEIFLTGTIVP"
     gene            212..1147
                     /locus_tag="PLI30_06130"
     CDS             212..1147
                     /locus_tag="PLI30_06130"
                     /EC_number="4.1.3.3"
                     /EC_number="4.2.1.41"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005637696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009436 - glyoxylate catabolic process
                     [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HOI79165.1"
                     /translation="MKPLSSDEIYGNWATLLLATDKNGEIDVSRMNDEIDVLIDSQPN
                     GIYSNGTAGEFYSQTDDEFLRISELLAVKCEKGGIPFQIGISHMSPQISLHRLKMITH
                     LKPGAVQVILPDWFPPTLEECAIFLKKIAAEAGEINLVLYNPPHAKKQLQPEEWQVLK
                     KGIPALVGVKVFDRNSDLEWYKRVKDNRDGISVFIPGHNLATGISLGAQGAYSNVSCL
                     NPRAAQKWYQLVRTDMSQALELEQRIKHFMKTLIEPFITQHHFSNHACDRFMALVGGW
                     ADVGASLRWPYKSIPEDLAGDVRKKAVNIIPEFFE"
     gene            1205..2809
                     /locus_tag="PLI30_06135"
     CDS             1205..2809
                     /locus_tag="PLI30_06135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005650665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exo-alpha-sialidase"
                     /protein_id="HOI79166.1"
                     /translation="MLTISCGYNDRVSAEIDSPTLPVLTGKKYNPVQKIRLIRDCTGF
                     NREEYILEKVTLSLEGTTDISDIDKVMLFRAGENDKLETDHPVGEVQPTATKITFSNH
                     FTVTEDTTILWVSVTLKKSIDLSHKIKIRCEEIVTDRGKLKILPHDAKELRAGVALRQ
                     HGQDGVHTSRIPGLATSKKGTLLAIYDARYESARDLQGHMDIALNRSFDGGRTWDPMQ
                     VVLDRKEWGGMPEKYNGVSDACILVDDRTGDIYVAGLWMHGLLDAETGKWVEGLTADS
                     TRWLHQWRAKGSQPGFGVKETSQFLITKSSDDGLTWSEPINITSAKKKEWWLFAPAPG
                     QGITMTDGTLVFPSQGRDENGTSFSNITWSKDGGETWKTSNPATKNTTECAVVELQDG
                     SLMLNMRDNRNRGNEEVNGRSVFVTSDLGETWKEHPTSRKALIEPTCMASLHTHRYME
                     DGVEKSLLLFVNPASTSKRNNITLKVSYDGGNTWPEEKQILLDEYSGRGYSCITSVND
                     STLGILYESSQADMAFQTVSLKEIQP"
     gene            2866..5502
                     /locus_tag="PLI30_06140"
     CDS             2866..5502
                     /locus_tag="PLI30_06140"
                     /inference="COORDINATES: protein motif:HMM:TIGR00813.1"
                     /note="Members of the Solute:Sodium Symporter (SSS), TC
                     2.A.21 as described in tcdb.org, catalyze solute:Na+
                     symport. Known solutes for members of the family include
                     sugars, amino acids, nucleosides, inositols, vitamins,
                     urea or anions, depending on the system; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015370 - solute:sodium symporter activity
                     [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/solute symporter"
                     /protein_id="HOI79167.1"
                     /translation="MIRKKISRIYLLIWMWLSISVTFLKADNLNTLHQINCFSMETLE
                     DSTLWEGLAGTFFGKQENRFIMAGGSAFPGGKPWQDGIKHFSNQVFVFEQLPDGSFSK
                     VYQGNDLPVPLAEGSYATLPGGLLCVGGLTPDGITGKSWLLAYSGSGITVTEYPSLPV
                     PVKNSTATVIGSKVYLVGGELADGTSTDQFLVLDLSNPTKGWEKLPNFPVPVSGATLT
                     SQQDGEEVALFSFGGRARRTAGEATIFYASVYHFRPSLNRWTQKGDIHHDKDTSVSLA
                     MATASAVGGSHVLLYGGDTGHIFNQVEKAVREGNTAKRDSLWIHHTGFHNKILIYNTI
                     TDTWFEAGKTVNPPVAVTSDVSDGKNVYIASGEIRPGVRSPLITQLYYTSQPSFGWIN
                     YLVLILYFGGMLLMGFYFMKRNENTEDFFKASGRIPWWAAGISIFATTLSAITFLSIP
                     AKTYATDWRMLVFNICIILIVPVVIRYFLPFFTRFRLGTAYEYLEKRFNRTTRWLASA
                     LFVVFMVSRIAIVLFLPALALNAVTGFDIYLSIVVMGLVTVIYSTSGGMEAVVWGDVI
                     QGFILVFGALAAFVLMVMGIDGGLGEFWHTSIEFHKFRTFDFRFDFTQPVFWVVLIGG
                     VANSLITYTSDQSIVQRYMSTKDEKSTSRSIWLNGILSVPVTILFFLIGTGLFAFYKD
                     NPASMMVTNPNIDSVFPQFIVSEMPVGFAGLLIAAIFAAAMSTLSSNINSSAAVLTSD
                     FYQTIFPKSTFSSQLKMARWSGLLCGLLGMGMALMLATWNIASLWDQFNTFLGLLTSG
                     MGALFILGIFFPRVGAGAALVGVITGLFLLLIIKEHTPLSFLLYGFIGVSVSILIAWL
                     SSFLLPNKKEIKGYTWKGMQKE"
     gene            complement(5696..5827)
                     /locus_tag="PLI30_06145"
     CDS             complement(5696..5827)
                     /locus_tag="PLI30_06145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI79168.1"
                     /translation="MGSSFTKSSMFDSIVFRDFDLLSLSDSLSILNLIPRFRFTVWL"
     gene            complement(6040..6504)
                     /locus_tag="PLI30_06150"
     CDS             complement(6040..6504)
                     /locus_tag="PLI30_06150"
                     /inference="COORDINATES: protein motif:HMM:NF025819.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mov34/MPN/PAD-1 family protein"
                     /protein_id="HOI79169.1"
                     /translation="MTQGKIEILIARSALNLIEKFKQTSKNANESGGILLGQVTDDKV
                     YILKVTTPNKFDKASRYSFDCNKDAAQVIIDYEFFNSGRKTIYIGEWHTHPENYPNPS
                     GVDKKMIDNQYFKNKLNEPFLILIIQGLKGLYIAIFNGKKLEETIVTIIEDN"
     gene            complement(6501..7373)
                     /locus_tag="PLI30_06155"
     CDS             complement(6501..7373)
                     /locus_tag="PLI30_06155"
                     /inference="COORDINATES: protein motif:HMM:NF013094.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ThiF family adenylyltransferase"
                     /protein_id="HOI79170.1"
                     /translation="MKHFDVISTVQSNENVERITTDVFTKERLENRTSGMVDQNIEQT
                     FLIAGVGSIGSNLIYFLNSMNSPSFKLIDNDEIKLENINRHLLGFEYIGYYKTKAIKE
                     FLLKNNPIQKVSTKESSIVDIINNETDYVNEADYIFISIGKANIDNWICQSLKDRILK
                     KPIFFIWVEPFLSGGHCIYLHPSNPEFEKFFDDDFFKFNVIDRIEYKLGNKNLSLREV
                     GCQTTYIPYSGANVISFISTLFPFISSIIDYNKTESVAYTWLGNTSEVKKIGIKISDL
                     YENKIIGAITEHKL"
BASE COUNT         2053 a         1639 c         1680 g         2138 t
ORIGIN      
        1 atctgacgct taaagttagc ctggacgact gccggagttg gagcaacagt aagctggtgt
       61 atgaggggcc ggctgcatac tcctgcatta ccaggttacc gaatggaaga gtgggccttt
      121 tttttgaagc cggagaaaaa agaccgtacg aaaaaatgat ctttgtctcc tttgactgga
      181 atgagatttt tcttacggga accatcgtac catgaagcca ctttcttcag atgaaatata
      241 cggcaattgg gcaacgctgc tcttggcaac tgacaaaaac ggagagattg acgtttcccg
      301 aatgaatgat gagattgacg ttttaatcga ttcacaaccc aacggaattt attcaaacgg
      361 gactgccggt gaattctatt cacagaccga tgacgagttt cttcggatta gtgaactact
      421 ggctgtgaag tgtgaaaagg gaggtatccc ttttcaaata ggaatcagtc acatgagtcc
      481 gcagatatcg ctgcaccggt tgaaaatgat tacacattta aagccgggag cggtacaggt
      541 tattcttccc gattggtttc cccccacttt ggaagagtgc gcgatatttc tgaaaaaaat
      601 tgcagcggaa gccggcgaga tcaatcttgt cctgtataac ccgccacacg cgaagaagca
      661 gttacagccc gaagagtggc aggttttaaa aaagggaata ccggctttgg tgggtgtgaa
      721 ggtgtttgac agaaattccg acttggaatg gtacaaaagg gtaaaagaca acagggatgg
      781 catttccgtg tttataccgg gtcataatct agcgaccggc atcagcctgg gtgcacaggg
      841 agcctattcc aacgtctctt gtctgaaccc acgggctgcc cagaaatggt atcaactagt
      901 gcgaaccgac atgagtcagg cgctggagtt ggaacaacgg atcaaacatt ttatgaaaac
      961 cctgatagag ccgtttatta cgcaacatca cttttccaat catgcctgcg atcgttttat
     1021 ggctttggta ggcggatggg ccgacgtagg cgccagcttg aggtggccct ataagtctat
     1081 tccggaagat ttggccggtg atgttcggaa aaaagctgtg aatatcatcc ccgaattttt
     1141 tgaataacaa cacatatatc tttttatcat gcaaagaaaa atactgtttt tactctctct
     1201 ggttatgctg accatctcat gtggatacaa cgatcgagta agcgccgaaa tagattcgcc
     1261 cactcttccc gttctgactg gaaaaaagta caacccggta cagaaaatca ggttgatccg
     1321 cgattgtacc ggcttcaacc gggaagaata tattcttgag aaagtgacgc tctcactgga
     1381 aggaacgacc gatataagtg acatcgacaa ggtaatgctg ttcagagcag gagagaacga
     1441 taaattagaa acagatcatc ctgtcggcga ggtgcagcct actgcaacga agattacttt
     1501 ttccaatcac ttcaccgtaa cagaagatac tactatcctc tgggtttcag ttaccctgaa
     1561 aaaaagtatc gatctgtcac acaaaataaa aatacggtgc gaagaaatag tcaccgaccg
     1621 gggaaaactg aaaattttac cgcatgatgc aaaggaactg agagccggtg tggcccttcg
     1681 gcaacatgga caggatggtg tacacacctc acgcataccc ggtctggcta cttcaaaaaa
     1741 aggtacgttg ttggcaatct atgacgcacg ctacgaaagt gcgcgcgacc tgcagggtca
     1801 tatggacatt gccttgaacc ggagcttcga tggcggacgc acttgggacc ccatgcaggt
     1861 ggtactcgat aggaaggagt ggggcggcat gccggaaaaa tataacgggg tgagcgatgc
     1921 ctgtatcctg gtggatgatc gaacaggaga catttatgta gccggcctgt ggatgcacgg
     1981 attgttggat gcggagacag ggaaatgggt tgagggattg actgccgaca gcacacggtg
     2041 gctacaccaa tggcgtgcga aaggttctca gcccggcttt ggtgtgaagg aaacctccca
     2101 gttcctgatt acgaaaagca gcgacgatgg cctcacctgg agcgaaccaa tcaacatcac
     2161 ctccgcaaaa aagaaggagt ggtggctctt tgcgccggca cccggacagg gaatcaccat
     2221 gactgacggc acgttggttt ttccatcaca aggtagggat gaaaatggca cctcattctc
     2281 taacattacc tggagcaaag atggaggaga gacatggaaa acaagtaacc cggcaaccaa
     2341 aaatacgaca gagtgtgccg tggttgaact ccaagacgga tcactgatgc tgaatatgcg
     2401 ggataaccgc aacagaggca atgaggaggt gaacggccga tcggtgtttg ttaccagtga
     2461 tctgggcgag acttggaaag agcaccccac ctcccggaaa gctcttatag aacccacctg
     2521 catggctagt ctccatacac atcgttatat ggaggatggt gtggaaaaat cgttgctact
     2581 gtttgtcaat ccggcatcta cctcaaaacg gaataacatc accctgaaag tgagttacga
     2641 cggtggcaat acctggcccg aagagaaaca gatcctgctt gatgaataca gcggacgtgg
     2701 ctattcctgc atcacatcgg tgaacgactc cacccttggc attctgtatg agagcagcca
     2761 ggctgacatg gccttccaga cggtttctct taaagagata caaccatgat gcgttcgaag
     2821 atttacaatt ttttattcac cacaaacttc atataaattt actgcatgat ccgaaagaaa
     2881 atttcacgta tctatctcct tatatggatg tggttatcca tatctgtcac tttcctaaaa
     2941 gcggataatc taaataccct tcaccagata aactgtttct ccatggagac attggaagat
     3001 tctaccctgt gggaggggct ggcaggtact tttttcggta aacaggaaaa ccggtttatc
     3061 atggctggag gatccgcttt ccccggaggg aaaccatggc aggatggaat caaacatttt
     3121 tcgaatcagg tatttgtttt tgaacaactc cctgacgggt ctttcagcaa ggtctatcag
     3181 ggaaatgacc tgcctgtacc cttggcggaa ggctcctatg ccacgcttcc cgggggactg
     3241 ctctgtgtgg gtggacttac cccggatggc atcaccggaa aatcctggtt gctggcatac
     3301 tccggcagcg gcatcactgt cacagaatat ccctcgctac cagtgccggt aaaaaattcc
     3361 acagctacag tcataggctc aaaggtttat ttggttggcg gcgaactggc cgacggcacc
     3421 tcaaccgacc agtttttggt gctcgacctc tccaatccga caaaaggatg ggaaaaacta
     3481 cccaattttc cagtaccggt ttcgggagct acccttactt cccagcagga tggggaagag
     3541 gtggctctct tctcttttgg cggaagggcc agaagaactg ccggagaagc cacaatcttt
     3601 tatgcatctg tttaccattt ccgaccttca ctcaacaggt ggacccaaaa aggagatatc
     3661 caccacgata aggacacttc agtttctttg gcaatggcta ccgcctcagc tgtgggtggt
     3721 tcacatgtgc tgctctacgg cggtgatact ggccatattt ttaatcaggt agagaaggct
     3781 gtccgggaag gcaatacagc taaaagagat agtttgtgga tacatcatac gggatttcat
     3841 aacaagatat tgatctacaa taccatcacc gatacatggt ttgaagcagg aaaaactgtg
     3901 aatcctcccg tggctgtcac atcggatgta tccgacggga agaatgttta tattgccagc
     3961 ggggaaattc gccccggagt gcgttccccc ctcatcacgc agctttatta tacctcacaa
     4021 ccctctttcg gatggatcaa ctacctggtg cttatcctct attttggagg aatgctgctg
     4081 atgggcttct acttcatgaa acgaaatgag aataccgagg atttcttcaa agcgagcgga
     4141 cgcattccct ggtgggccgc gggaatcagt atttttgcca caacgctgag tgctattaca
     4201 ttcctgtcga tacctgcaaa gacttatgca accgactggc gtatgcttgt ctttaatatc
     4261 tgcatcattc tcattgtccc ggtggtcatt cgttattttc ttcctttctt tacccgcttc
     4321 cggttgggta ctgcttacga atacctggag aaacgcttca accgcaccac aagatggctg
     4381 gcttctgccc tgtttgttgt ctttatggtg agccgcattg ccattgtgct gtttttgccg
     4441 gcactggcac tgaatgcggt gacagggttc gatatttatc tctctatcgt agtcatgggg
     4501 ttggtaaccg ttatctacag tactagcggc ggtatggagg ccgttgtctg gggagacgtc
     4561 atacagggat ttattcttgt ctttggagca cttgctgcat ttgttttgat ggtaatgggt
     4621 atcgatggag gcttgggaga attctggcat acttccatcg agtttcacaa attccggacg
     4681 ttcgatttcc ggtttgattt tacacagccg gtattctggg tcgtattgat tggcggagtg
     4741 gctaattcgc tcatcaccta taccagcgat cagtctatcg tacagcgtta tatgagcaca
     4801 aaagatgaaa aatccacttc ccgcagcatc tggctgaatg gtatcctaag tgttccggtc
     4861 accattttgt tttttctgat aggaaccggc cttttcgcat tctataaaga taaccccgca
     4921 agcatgatgg ttaccaatcc caatatcgat tcggtatttc cgcagtttat cgtgtcggag
     4981 atgccggtag ggtttgccgg actgctcatt gccgccattt ttgccgcagc catgtcaacc
     5041 ctttcatcca acatcaactc ctctgccgcc gtgctcacat ccgatttcta ccagactatt
     5101 ttcccgaaaa gtactttttc atcacaactg aaaatggccc gctggtcggg cctcctctgc
     5161 ggattactgg gaatgggtat ggcattgatg ctggccacct ggaacatcgc ttctttgtgg
     5221 gaccagttca acacgttcct gggacttctc accagcggaa tgggggccct ctttatcctg
     5281 ggaatattct ttccccgggt gggtgcaggg gcagcccttg tcggcgtgat cacagggctc
     5341 ttcctgctac tgattatcaa ggagcatact cccttgtcgt ttttgctgta cggttttatc
     5401 ggagtgtcgg taagtatcct gattgcctgg ctgtcaagtt ttttgttacc caacaaaaag
     5461 gagatcaaag gatacacctg gaagggtatg cagaaagaat gacatgctac ccctttttta
     5521 gggacaagat aacttgatct tttcaaagtg gcacaaatca aaccgtaaaa agtggcacac
     5581 agaccgaaat cactggcaca aatcaaaccg ttatatccag tggtgtggag ttcctgcgca
     5641 gatttgccca acacatcctc ccgcgcagat ttgtaaagat ccgacgttac ggtatctaca
     5701 accacacggt gaaacggaac ctgggaatca ggttcaggat agaaagtgaa tcggaaagcg
     5761 acaacaaatc aaaatcccgg aaaacgattg aatcgaacat tgaacttttc gtaaaactgc
     5821 ttcccatacg ttttccaccg ggagccacct gcaaacagac ttatctgact atgacatata
     5881 cagattgtta ccgcctggtg tttatttttt cagtcttttt attctcaatt tactattgtt
     5941 tctctgtctg acgtgcgttg cataggcaag cttttttgca ggttttggtc atgcgttggt
     6001 tggtgcgacc tgcaaatgtg cttggctaag aagcgtgggc taattatctt ctattattgt
     6061 tactatcgtt tcttcaagtt ttttcccatt aaaaatagca atatataaac cttttaatcc
     6121 ttgaattatt aagattaaaa atggctcatt taatttgttc ttaaaatatt gattatctat
     6181 catttttttg tcaactcctg aaggatttgg ataattttct ggatgagtgt gccattctcc
     6241 aatataaatt gttttacgcc cactattaaa aaattcatag tcaattataa cttgtgcagc
     6301 atctttgtta caatcgaatg aatatctgct tgccttatcg aatttattgg gagttgtaac
     6361 cttaagtatg taaactttat catctgttac ttgtcctaat aaaatacctc cagattcatt
     6421 tgcattcttt gaggtttgtt taaacttctc aattaaattt aaagctgaac gagctattag
     6481 tatttcaatt tttccttgag tcatagttta tgttctgtaa tagcacctat gattttgttc
     6541 tcatataaat cagaaatttt aattcctatt ttttttactt ctgacgtatt gccaagccaa
     6601 gtataagcaa ctgattctgt tttattatag tcaattatag atgaaataaa agggaatagt
     6661 gtagaaataa aactgattac gtttgctcct gaatatggaa tgtaagttgt ttgacatcct
     6721 acttctcgaa gtgaaagatt tttattgcct aatttatatt caatcctatc aattacatta
     6781 aatttaaaaa aatcgtcatc aaaaaacttt tcaaactctg gatttgaggg gtgtaggtaa
     6841 atacaatgac caccacttaa aaatggttcc acccaaataa aaaatattgg ctttttaagt
     6901 attctatctt ttagtgattg gcaaatccaa ttatcaatat ttgctttgcc gatagaaatg
     6961 aatatgtagt ctgcttcatt gacatagtct gtttcattat taatgatgtc aacaattgag
     7021 ctttcttttg tactaacttt ctgaattgga ttatttttta acaggaattc tttaatagct
     7081 tttgttttat aatatccaat atactcaaat ccaagaaggt gtctgtttat gttttcaagt
     7141 ttaatctcat cattatcaat taatttaaac gatggcgaat tcatagaatt taaaaaatag
     7201 attaaattag aaccaatact acctacacct gcaattaaaa atgtttgttc aatattttga
     7261 tcaaccatac ctgaggttct gttttctaat ctttcttttg taaacacatc tgttgtgatt
     7321 ctttctacat tttcatttga ttgaactgtt gaaataacat cgaaatgctt caatgccccc
     7381 ggtcgaaatc ctttaacaaa agtgtttatg cttttgtgaa accacccgaa taaataagtt
     7441 ttttcaccaa aatactgtaa gcacccgagc aactacctct tgattattcc ggagcatacg
     7501 cacccacgga
//
LOCUS       DAOSDR010000122         7503 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrimonas sp. isolate AMR_MDS_1826
            NODE_3606_length_7503_cov_3.435150, whole genome shotgun sequence.
ACCESSION   DAOSDR010000122 DAOSDR010000000
VERSION     DAOSDR010000122.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863355
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrimonas sp. (wastewater metagenome)
  ORGANISM  Petrimonas sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Dysgonomonadaceae; Petrimonas.
REFERENCE   1  (bases 1 to 7503)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7503)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:10:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,720
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 35
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7503
                     /organism="Petrimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3606_length_7503_cov_3.435150"
                     /isolate="AMR_MDS_1826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2023866"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..531)
                     /locus_tag="PLI30_06160"
     CDS             complement(<1..531)
                     /locus_tag="PLI30_06160"
                     /inference="COORDINATES: protein motif:HMM:NF011094.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HOI79171.1"
                     /translation="MATKLRLQRRGRKNYAFYQIIVADSRAPRDGKYIERIGSYNPNT
                     NPATITLDFDRALYWLQVGAQPTDTVRNILSDEGVLMKKHLLGGVAKGAFDEAEAEKR
                     FEAWMNSKLQASQKLKNKDEEAQRAAEKARLDAEKEVNKAKAEALAKKKAEAEAENNP
                     VAEETVEETVEAAETTE"
     gene            complement(612..684)
                     /locus_tag="PLI30_06165"
     tRNA            complement(612..684)
                     /locus_tag="PLI30_06165"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(649..651),aa:Lys,seq:ttt)
     gene            complement(796..2214)
                     /gene="ahcY"
                     /locus_tag="PLI30_06170"
     CDS             complement(796..2214)
                     /gene="ahcY"
                     /locus_tag="PLI30_06170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019540891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004013 - adenosylhomocysteinase activity
                     [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HOI79172.1"
                     /translation="MDKNFSLDLPYKIADISLAGFGRKEIEIAEHEMPGLMSIRKKYA
                     SEKPLQGARITGSLHMTIQTAVLIETLVELGADVRWASCNIFSTQDHAAAAIAAAGIP
                     VYAWKGETLEEYWWCTEMALRFPGGKGPNLIVDDGGDASLLVHLGYRAEKDAKTIDRK
                     GANREEQVVLDTLNRILREDSQRWHRTVAELKGISEETTTGVHRLYQMMEKGELLVPA
                     INVNDSVTKSKFDNLYGCRESLADGIKRATDVMIAGKVVVVLGYGDVGKGCAKSMRSY
                     GARVIVTEIDPICALQAAMEGFQVTTMEEAVKEGNIFVTTTGNRDVVTIEHMHNMKDQ
                     SIVCNIGHFDNEIQVDKLISFPGIAHTNIKPQVDKYTFPKGNSIFLLAEGRLVNLGCA
                     TGHPSFVMSNSFANQTLAQIDLWKNDYETGVYRLSKQLDEEVARLHLEQIGVKLTTLT
                     PEQAGYIGVNIDGPYKPEHYRY"
     gene            2375..2674
                     /locus_tag="PLI30_06175"
     CDS             2375..2674
                     /locus_tag="PLI30_06175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004359976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI79173.1"
                     /translation="MKGFRAINLFGIIFARKEQPELSRQIINHELIHTRQMLELLVIG
                     FYVWYITEWVIKWIIYKNRFLAYRNIGFEREAFDNDANLDYLKYRRRYGFAGYVL"
     gene            2860..3708
                     /locus_tag="PLI30_06180"
     CDS             2860..3708
                     /locus_tag="PLI30_06180"
                     /EC_number="1.8.4.11"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methionine sulfoxide reductase B/A
                     protein"
                     /protein_id="HOI79174.1"
                     /translation="MGYNQLTPEEEFVILHKGTERPFTGELLENKAKGTYVCKRCDAP
                     LYRSEDKFESNCGWPSFDDEIVGAVKRVRDADGRRTEILCNNCGAHLGHVFLGEGFTA
                     KQTRHCVNSISMKFIPAGHTDLRKGYFASGCFWGTEYFFMKAKGVKETHVGFMGGHVD
                     NPTYEQVCQKNTGHLETTEVVFDPAETTYEEMVKLFFETHDFTQTNGQGPDIGPQYLS
                     CIFYSTPREKEIAEKYIALLTNKGYKVATTLKPASTFWKAENYHQQYYEHKGTKPYCH
                     KYTKVF"
     gene            3813..4298
                     /locus_tag="PLI30_06185"
     CDS             3813..4298
                     /locus_tag="PLI30_06185"
                     /inference="COORDINATES: protein motif:HMM:NF016836.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HOI79175.1"
                     /translation="MKVFRLVSMLALILAVGVATTSCKKVKDADVQAAAQMALVANPD
                     LADVSVAVLDQVATLSGTVKDDASKAYAETTVAAVENVKSVVNNIEVVPPAPDFTAID
                     AALNSGLADALKDHATVKAEVKDGVVTLTGEIRKRDLQKLMEKVNALNPQQVVNNITV
                     K"
     gene            4307..4717
                     /locus_tag="PLI30_06190"
     CDS             4307..4717
                     /locus_tag="PLI30_06190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019540895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HOI79176.1"
                     /translation="MALQEKYKELVDLARQYGATVSDEGNVLRVRGEVPSGALKDKMW
                     EIYDRIDPNFKNNDLILDVNVKATEGGKVKVVTRESNLNIRKGPGTDQPIVGKAAHGD
                     VITLISKANNQWWLVRDNDGEEGYCYSQYLEPVQ"
     gene            4902..5645
                     /locus_tag="PLI30_06195"
     CDS             4902..5645
                     /locus_tag="PLI30_06195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013612870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOI79177.1"
                     /translation="MQTSTNQTGQYTFTIIVPVYNEEDNIARLEEKLSNYLPQSLYKT
                     CVLFVNDGSTDDSGSRIHQMCDRHPGFFYIEMKRNGGLSAALKAGFDYCFSEYVGYMD
                     ADMQTDADDFNLLLPGLANCEFVTGIRANRKDSFFKNLQSKIANGYRRMMTGDGVSDT
                     GCPLKVMYTDYAKRIPMFKGMHRFLPALILLQNGNVQQIPVRHYPRIAGKSKYHLWNR
                     LTGPFIDCFAYRWMKKRYINYRIVASNLH"
     gene            5648..6295
                     /locus_tag="PLI30_06200"
     CDS             5648..6295
                     /locus_tag="PLI30_06200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005802698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase N-terminal
                     domain-containing protein"
                     /protein_id="HOI79178.1"
                     /translation="MQGSPAWVYAIGFLAQAFFSARLLYQWIVTEKAKKVLSPAAFWI
                     LSIFGSYLLFIYGVLRNDFAIILGQFISYYIYLWNLDLKGLWKRLPFALKSVLLLTPL
                     AATFFLMRDIPAFTAAFFQNKNVPFWLLVFGSVGQVLFTFRFIYQWIYSTHRRESALP
                     IGFWVISLLGSASIIAYGIFRIDPVLILGQSFGFVAYIRNIMIDYSNKKHISDEE"
     gene            6285..>7503
                     /locus_tag="PLI30_06205"
     CDS             6285..>7503
                     /locus_tag="PLI30_06205"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019540445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HOI79179.1"
                     /translation="MKNKYLYLIWFVALLPVMILRDFTPDNELRYLSIVDEALQNGDV
                     FTFTNQGEIYADKPPLYFWLMMAGKTLLGGHRMWFLSLLSFVPAVIVLTTMAKWVRKE
                     NAENQTEALLMLMTAGLFTGLALVVRMDMLMCMFIVLALYTFFKIYKGEGGKRDSFLF
                     PVYVFLALFTKGPVGILVPLLSTLVFLLYQRKIHTWRQYWGWKSLLVLLLGCGIWFAG
                     AYMESGSEYLNNLLVHQTVGRGINAFHHKRPFYYYVLSVWYSLAPWMFLSVGLIAFGG
                     VRRKIQTEIERFFLIIILTTFVMLSVISSKLAVYLLPSFPFFIFLPALLLKKFDPTNI
                     WLRISLAAPALVFVLALPALVYLSGTDDAFFAGVFLVYLAAGIITLSGLTVLYLLFFK
                     KQLQHPIRVMAAGV"
BASE COUNT         1888 a         1606 c         1764 g         2245 t
ORIGIN      
        1 ttcagttgtt tctgcagcct caacggtctc ttctactgtt tcttctgcaa ccggattgtt
       61 ttcagcttcc gcttcggctt ttttcttcgc caatgcttcc gcttttgctt tgttcacttc
      121 tttttcagca tccaggcggg ccttttctgc tgcacgttgt gcttcttcgt ctttgttttt
      181 cagcttttgg ctggcttgca gtttggagtt catccatgct tcgaatctct tttcagcttc
      241 tgcctcatcg aaagcgccct ttgctacacc tcccaggaga tgttttttca tcaatactcc
      301 ttcgtccgac aaaatattac ggacggtgtc tgtgggttgc gcacccactt gcagccaata
      361 caaagctctg tcgaaatcta aagtgatagt agcaggattt gtgttcggat tgtaagatcc
      421 gatcctttca atgtactttc catcacgtgg agctctgcta tctgcaacga taatctggta
      481 gaaggcgtaa tttttacggc ctcttctttg taaacgcagt tttgttgcca tttagtagtt
      541 tttgtgaatg ttagaaattt tgtatgtatg aaataaaact cctgtttttt tgtgcaggag
      601 ttcagtattc gtgatccgcc tggggctcga acccaggacc ccatccttaa aagggatgtg
      661 ctctacctgc tgagctagcg aatcagccta aatgctttct ttcaaaagcg agtgcaaagg
      721 taaatcaaaa tattttaata agcaaaactt gtggaaataa aattgcaata aatttttaac
      781 agttctgcta acgtgttaat acctataatg ttccggcttg tatggcccgt cgatatttac
      841 cccgatatag ccggcctgtt cgggggtaag tgttgtcagt ttcaccccga tttgttccag
      901 gtgaagccgg gcaacttctt catccagctg tttggaaagc cggtatacac ctgtttcgta
      961 atcgtttttc cacaaatcaa tctgtgccag ggtttggttg gcaaacgaat tgctcatcac
     1021 aaacgaagga tggccggttg cacaacccag gtttaccaga cggccttcgg caagcaggaa
     1081 tatggagttc cctttaggga aagtatattt gtctacctgc ggtttgatat tagtgtgagc
     1141 gatccctgga aaactgatca gtttgtccac ctgtatttcg ttgtcgaaat gcccgatatt
     1201 gcacacaatg gattggtctt tcatgttgtg catatgctcg atggtaacca cgtcccggtt
     1261 tccggtggtg gtcacaaaga tgttgccttc tttcacggct tcttccatgg tggttacctg
     1321 aaatccttcc atggcggcct gcagggcaca tatgggatcg atctccgtaa cgataaccct
     1381 ggctccgtag gagcgcatgg atttggcaca gccttttccc acgtcgccgt aacccagcac
     1441 gaccactact tttcctgcaa tcatcacgtc ggtggctcgt tttatgccgt cggccagtga
     1501 ttcgcggcaa ccgtacaggt tgtcaaattt cgatttggta accgaatcat tgacgttgat
     1561 ggcgggaacc agcagctccc ctttttccat catctggtac aaccggtgaa cgccggttgt
     1621 ggtctcttcc gaaataccct tcagttcggc aacagtgcgg tgccagcgtt ggctgtcttc
     1681 tctcagtata cggtttaacg tatccagaac tacctgttct tcacgattgg ccccttttct
     1741 atcaatggtt ttcgcatctt tttctgcccg ataacccaag tgcacgagca acgaagcatc
     1801 tcctccgtcg tcaacgatca ggttcggacc tttcccgccg gggaatcgta gtgccatctc
     1861 cgtacaccac caatattctt ccagcgtttc gcccttccat gcataaacgg ggattccggc
     1921 tgcggcaatc gcagcagcag cgtggtcttg agtggagaat atgttgcaac tggcccaccg
     1981 tacgtcggct cctaattcaa ccagtgtttc tatcagtacg gcagtctgta tggtcatgtg
     2041 cagtgaaccc gtgatgcggg ccccctggag aggtttttcc gatgcatatt ttttgcggat
     2101 tgacatcaac ccaggcattt cgtgttcagc aatttcaatt tccttccgtc cgaaaccggc
     2161 cagggagata tccgctattt tataaggtaa atccaaagaa aaatttttat ccatttccgt
     2221 tttatttatt tttaagccta caaagttaca aaatttacag aatgttttta cctttgaaac
     2281 cagtaatcac tgaatttgtt actgaatttg agcatgtgtt acggtatcag caaatattga
     2341 cagcttgaag attatctatt ctaaatacct tcctgtgaag ggttttcgcg cgatcaatct
     2401 ttttgggatc atttttgcca gaaaggaaca gccggagtta agcaggcaga ttatcaacca
     2461 cgagttgatt catacccggc agatgctgga gttattggtt atcggatttt atgtctggta
     2521 cattacggaa tgggtaatta aatggattat ttacaagaac cggtttctgg cgtacagaaa
     2581 cattggattt gagcgtgaag catttgataa cgatgcgaat ttagattact tgaagtatcg
     2641 gagacggtat ggatttgcag gttacgtttt gtaacaagat acatttcact taaaaatcac
     2701 agaaatatgg aaccgacaaa aattccacct gactttcatg caaaaaacaa gcaggtgaaa
     2761 tgtctgcgga tatttttttt actggctctt gtcagcacaa ccttcaccat gtgtgcgcaa
     2821 aaaagtaaaa aggaaatcaa tttaaaacaa tatataacta tgggatataa tcaattgact
     2881 cctgaagaag agtttgttat tttacataaa ggaaccgaac gccctttcac gggagaactg
     2941 ttggaaaaca aagccaaagg aacctacgtt tgtaaacggt gcgatgcgcc gctctaccgt
     3001 tccgaagaca agttcgaatc gaattgcggg tggccgtctt tcgacgatga aattgtcggt
     3061 gctgtaaaac gcgtccggga tgcagatgga cgacgtaccg aaattctttg caacaattgc
     3121 ggggcgcacc tgggtcacgt ttttctgggc gagggtttta cggccaagca gacgcgccat
     3181 tgcgtgaact ccatctccat gaagtttatt cctgcaggcc atacggatct gcgaaagggt
     3241 tactttgcct ccggctgttt ctgggggact gaatattttt ttatgaaagc caaaggcgtg
     3301 aaagagaccc atgtcggatt tatgggcggc cacgtggata atcccaccta cgaacaggtt
     3361 tgtcagaaaa atactggaca cctggagacg actgaagtgg tttttgatcc ggcggaaaca
     3421 acctatgaag aaatggttaa gctgttcttc gaaacccacg atttcacaca aaccaacgga
     3481 caaggccccg atattggccc gcagtacctg tcctgtatct tctattcaac acctcgggag
     3541 aaagagattg ctgaaaaata catcgcccta ctgacaaata aaggatacaa agtggctaca
     3601 acacttaagc cggcttctac attctggaaa gcagagaatt accaccagca gtactacgag
     3661 cataagggaa caaaaccgta ttgccacaag tataccaaag ttttctgaat ttattgtttt
     3721 ttataaaact tttcgatgat ctgatgcgtt tattacatgt attgtttaaa tataaaatga
     3781 tcaaaagttt aactaactaa aaacagtaaa ttatgaaagt atttcgtttg gtatcgatgc
     3841 tcgcattgat ccttgcagtt ggggttgcaa caacctcctg caaaaaagta aaagacgctg
     3901 atgtgcaagc tgctgcccaa atggcgttgg tcgctaatcc cgatttggca gacgtttcag
     3961 tggctgtatt ggatcaggta gccacactct cgggaacggt aaaagatgat gcatccaaag
     4021 cttacgcaga aactactgtg gcagcggttg aaaatgttaa atcggtggtt aataacatcg
     4081 aagttgttcc tcctgcccct gattttacag ctattgacgc tgcccttaat tccggcttgg
     4141 ctgatgcgtt gaaggaccac gcaaccgtta aggcagaagt aaaggacggt gtggttacgt
     4201 tgacaggtga aattcgcaag agggatttac agaagttgat ggaaaaggtt aacgcgctta
     4261 atccgcagca agttgtcaat aacattactg taaaataacc ggagatatgg cattacagga
     4321 aaaatacaag gaactggttg accttgcccg gcaatatggc gcaaccgtaa gtgatgaagg
     4381 caacgtgtta agagtgaggg gagaggtccc ctcgggtgct ttgaaagaca aaatgtggga
     4441 aatctacgac aggattgatc ccaattttaa aaacaacgac ctgatactcg acgtaaatgt
     4501 taaagctacc gaaggtggaa aagtaaaagt ggttacccgt gagagcaacc tgaacatccg
     4561 taaaggccct ggaacagacc agccgattgt gggaaaagca gctcacggag atgtgatcac
     4621 gcttatcagc aaagcgaaca atcaatggtg gttggtacgt gacaacgacg gtgaagaggg
     4681 gtattgctac tcccagtacc ttgaacccgt acagtaggct gaaggcattt gatggagaaa
     4741 agcttccgtt cctaaaagga gcggaagttt ttttatggaa aaatgatgct tgattcatgt
     4801 ataaactttg cggtaaacat tccggctgaa cagaaaaata actacatttg cagattaatt
     4861 tatcgtaaag tcaacgatta cttttgagtt gatttaaagt tatgcagacc agtacaaatc
     4921 aaacggggca gtacaccttt accattattg tccctgttta taatgaagaa gacaacattg
     4981 cccggttaga agaaaaactc agcaattatt tgcctcagtc gctttataaa acgtgtgtct
     5041 tgtttgtgaa cgatggttca acggacgata gtggaagccg catacaccag atgtgcgaca
     5101 gacatcccgg ttttttctat attgagatga aaagaaacgg cgggctcagt gctgcgctta
     5161 aagctggctt cgattactgc ttttcggaat acgtagggta catggatgcg gatatgcaaa
     5221 ccgatgcgga tgattttaac cttctgctcc ccggtttggc taattgtgaa tttgttacgg
     5281 gtattcgtgc caaccgcaag gattcttttt tcaaaaacct gcaatcgaag atagcgaacg
     5341 gttaccggcg gatgatgacg ggagatggtg tttctgatac ggggtgcccg cttaaagtga
     5401 tgtatacaga ttatgccaag cgtattccta tgttcaaggg gatgcaccgg tttcttccgg
     5461 cgctcatttt gttgcagaac ggcaacgtgc aacaaattcc tgtaaggcat tatccacgta
     5521 tagcgggaaa atcgaaatat catttgtgga atcgcctcac cgggcctttt atcgactgtt
     5581 ttgcctatcg gtggatgaag aaaaggtaca taaactaccg catcgttgcc agtaatttac
     5641 attgattatg caaggaagtc cggcctgggt atacgcgatt ggttttctgg cgcaagcttt
     5701 tttttcggca cggctgttgt accaatggat tgtcaccgaa aaggccaaaa aagtactttc
     5761 gcccgcagca ttctggatac tgagcatttt tggatcgtac ctgctgttta tttacggtgt
     5821 gctcaggaat gattttgcga ttattctggg acagtttatt tcgtattaca tctatttgtg
     5881 gaacctcgac ctgaaagggc tctggaagag gctccccttt gccttaaaat ccgttctgtt
     5941 gcttaccccc ctcgctgcaa cgtttttcct gatgcgggat attccggcct ttaccgccgc
     6001 tttttttcag aacaagaatg ttcctttctg gttgttggtc ttcggttctg tcggccaggt
     6061 cctgtttact ttccggttta tttatcaatg gatatattcg acacatcgtc gtgaatctgc
     6121 actgcccatt ggcttctggg tgatcagcct gttgggatcg gccagcatca ttgcctacgg
     6181 gatttttcgg atcgatccgg tattgattct ggggcaatcg tttggtttcg tggcgtatat
     6241 ccggaatata atgattgatt attcaaacaa aaaacatata tcggatgaag aataaatacc
     6301 tgtatcttat ttggtttgtt gccttacttc cggtaatgat cctccgtgat tttacgcccg
     6361 ataacgagct gcggtattta agcattgtgg atgaagcgtt gcaaaacggt gatgttttca
     6421 cgtttaccaa tcagggtgaa atttatgccg ataaaccgcc gctctatttt tggctgatga
     6481 tggccggaaa aacattgctt ggcgggcatc ggatgtggtt tttgtccttg ttgtcgtttg
     6541 ttccggcggt aatagttctc acgactatgg caaaatgggt ccggaaagaa aacgcagaaa
     6601 atcagaccga agctttgctg atgttgatga cggcggggtt gtttaccggc cttgctcttg
     6661 tggtgcggat ggatatgctg atgtgcatgt ttatcgtatt ggcgctctat acgttcttta
     6721 aaatatataa aggggaagga ggaaaacgcg actctttttt gtttccggta tatgttttcc
     6781 tcgcactttt tacgaaaggg cctgtgggaa tcctggtccc gttgttgtcg acacttgtct
     6841 ttcttctgta tcaacgaaaa attcatacgt ggaggcagta ttggggttgg aaaagcctgc
     6901 tggtactgtt gttgggttgc ggtatctggt ttgccggagc ttacatggaa tcggggagtg
     6961 agtacctcaa taacctgttg gtgcatcaga cggtgggcag gggaatcaac gcctttcatc
     7021 acaaacgccc gttttattat tacgtcctgt ctgtttggta ctcgttggca ccgtggatgt
     7081 ttctctccgt cggattgatt gcctttgggg gagtacgaag aaaaattcaa accgaaattg
     7141 agcgtttctt cttgattatt atactgacaa cgtttgtcat gctgtcggtt atcagttcga
     7201 agctggccgt ttacctgctt ccttcttttc catttttcat tttccttccg gcacttctgt
     7261 tgaaaaaatt tgatccaacg aatatctggt tgcgtatttc gttggctgct ccggcacttg
     7321 tttttgtgtt ggccttaccc gctttggttt atctctctgg aacagatgat gctttttttg
     7381 ccggtgtttt tctggtttat cttgctgcgg gcatcatcac gctcagcggg ttaacggttt
     7441 tatacctgtt gttttttaaa aaacagttgc aacacccgat ccgggtgatg gctgccgggg
     7501 tgc
//
LOCUS       DAOSDR010000123         7484 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrimonas sp. isolate AMR_MDS_1826
            NODE_3617_length_7484_cov_3.675999, whole genome shotgun sequence.
ACCESSION   DAOSDR010000123 DAOSDR010000000
VERSION     DAOSDR010000123.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863355
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrimonas sp. (wastewater metagenome)
  ORGANISM  Petrimonas sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Dysgonomonadaceae; Petrimonas.
REFERENCE   1  (bases 1 to 7484)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7484)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:10:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,720
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 35
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7484
                     /organism="Petrimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3617_length_7484_cov_3.675999"
                     /isolate="AMR_MDS_1826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2023866"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..327
                     /locus_tag="PLI30_06210"
     CDS             <1..327
                     /locus_tag="PLI30_06210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008023034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urocanate hydratase"
                     /protein_id="HOI79180.1"
                     /translation="MAVQCFAGNAARGMSLVALHNGGGVGIGKAVNGGFGMVCDGSER
                     VDEILRSAMLWDVMGGVARRSWARNANAMSTVQDFNQSFADDYYITEPYLVDEEIIKN
                     ITNYKQ"
     gene            367..1263
                     /gene="ftcD"
                     /locus_tag="PLI30_06215"
     CDS             367..1263
                     /gene="ftcD"
                     /locus_tag="PLI30_06215"
                     /EC_number="2.1.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013546696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030409 - glutamate formimidoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0030412 - formimidoyltetrahydrofolate
                     cyclodeaminase activity [Evidence IEA];
                     GO_process: GO:0019556 - histidine catabolic process to
                     glutamate and formamide [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate formimidoyltransferase"
                     /protein_id="HOI79181.1"
                     /translation="MNKIMECVPNFSEGRDKEKIEKIVECFRAKKNVKLLDYSNDEDH
                     NRLVVTVIGEPEPLKKSVVDAIGKAVELIDMNQHSGQHPRMGAVDVVPFIPIKNVTPE
                     EAVSFSKEVAQTVAERYNLPVFLYEKSASAPHRENLANIRKGEFEGMAEKIKKDDWKP
                     DFGPAERHPTAGAVAVGVRMPLVAYNVNLGTDNLEIAQSIAKKVRFIGGGLRFCKGMG
                     VALEERGITQVSMNLTDYTKTAIYRAHELVRIEANRYGVPVIGAEIVGLVPLEALVDT
                     AAYYLGLENFSLNQVLETKLME"
     gene            1278..1859
                     /gene="pnuC"
                     /locus_tag="PLI30_06220"
     CDS             1278..1859
                     /gene="pnuC"
                     /locus_tag="PLI30_06220"
                     /inference="COORDINATES: protein motif:HMM:TIGR01528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015663 - nicotinamide mononucleotide
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015890 - nicotinamide mononucleotide
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HOI79182.1"
                     /translation="MDWIEPIAAVFGVLSVWFARKENILVFPFGIINVAIYIYICFAA
                     RLYANAGINVVYLASNVYGWYLWSRIDGESRKLQIKRNTSKENMISWTAVVAIYVSLF
                     FLLRYVNRTDTGYIQSYLPYIDSFNTAFFLVATLLMAFKKLENWQFWIIGNIVSIPIY
                     ASQGLYFTSAQYAIFLVLAISGWKEWKRKINYK"
     gene            1865..3109
                     /gene="hutI"
                     /locus_tag="PLI30_06225"
     CDS             1865..3109
                     /gene="hutI"
                     /locus_tag="PLI30_06225"
                     /EC_number="3.5.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019540773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050480 - imidazolonepropionase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazolonepropionase"
                     /protein_id="HOI79183.1"
                     /translation="MNNLVVKNASQVVTCSGFTGKRGEDMSDLHVIENGTVVVTDGVI
                     SHVLKEGEGLSVDLSDYEVIRAEGKALLPGFVDPHTHFVFGGYREEEFAWRMRGDSYM
                     DIMNRGGGIVNTTRATRAASEDELFIAGKQRLDAMMRLGITTVEGKSGYGLDKETELK
                     QLRVMQRLNEEHPLDVVSTFMGAHATPEEWKGREDEFIDFNINEMFPLVAQEKLAECA
                     DIFCEKNVFTIEQSRRYLTAAREQGFKLKIHADEIVPFGGAELAAELGCLSADHLLKA
                     SGKGIRAMAKAEVVSTLLPLTAFSLRENFARARTMIDNNCMVALATDFNPGSSFSASV
                     PLLFALACIYMKMSPEEAVTAFTINSAAAVGRADKIGSIDVGKQADLVLLQFPSYKFL
                     PYHVGMNIVEKVIKRGCLLVDR"
     gene            3134..3760
                     /locus_tag="PLI30_06230"
     CDS             3134..3760
                     /locus_tag="PLI30_06230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019540775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclodeaminase/cyclohydrolase family protein"
                     /protein_id="HOI79184.1"
                     /translation="MKLQDLTLKQFLEKTAGNEPVPGGGSVSALNGAISSSLVQMLAN
                     LTIGRKNYTDVEERMKKNAGRFEEFRMHFLNDIDRDSEAYNLVFEAFKLPKETDEEKA
                     YRSQNIQEATKIAALVPMEIAERAFGMLDLIVETTRKGNKNAITDGCVAMMTCRTAVL
                     GALLNVRINLGGLKDAGFVQKLTDACSRIERETEAKEKELTDWVKTQL"
     gene            3768..5267
                     /gene="hutH"
                     /locus_tag="PLI30_06235"
     CDS             3768..5267
                     /gene="hutH"
                     /locus_tag="PLI30_06235"
                     /EC_number="4.3.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019540776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004397 - histidine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ammonia-lyase"
                     /protein_id="HOI79185.1"
                     /translation="MKNIYYIGKDELTFDDLERIINENIKLELSQEAKERIQKCRDYL
                     DQKMQNQQEPIYGISTGFGSLCNRTISNNDLHMLQENLVKSHACSVGEEVKPVIVKLM
                     FLLKAHALSLGYSGVQVATVQRMLDLFNNDILPVVYDKGSLGASGDLAPLANLFLPLI
                     GVGDVYYKGQKRDIGSVLDEFGWEPLKLKSKEGLALLNGTQFMSAHGVFAVMKAQRLS
                     KRADLIAAISLDAYDGHLEPFDEKLHLIRPHLGQLETSQNIVRFLEGSELISREKKHI
                     QDPYSFRCIPQVHGATKDAIAYVKSVLFTEINSVTDNPTIFPDDDQIISGGNFHGQPL
                     AITFDFLALALAELGNISERRVAQLILGNRGLPEFLVANPGLNSGFMIPQYVSASIVS
                     QNKMYCYAASSDSIVSSNGQEDHVSMGATSAIKLLKVMDNLDLILSIELMNAVQAMEF
                     RRPLKSSPVIEKTIKEYRKEVPFVEEDIVMYKEIHKTVAFLNRLHVDAL"
     gene            5333..6772
                     /locus_tag="PLI30_06240"
     CDS             5333..6772
                     /locus_tag="PLI30_06240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013611959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOI79186.1"
                     /translation="MVEKIHSTLRDSSVARWTALILLASTMFVAYMFIDVLAPLSTML
                     ETSLKWTPDTFGAVAGSEYFLNVFAFFLIFAGIILDRIGVRKAALVSGSLMVIGASIK
                     LYGISDYFNGGGFGYDFFNSFWTGFPASAKVASVGFAIFGCGVEMAGVTVSKGIVKWF
                     AGKELALAMGLEMAIARLGVFAVFRLSPYLAEKGGVSLSVGVGTLLLLIGLLTFSVYF
                     FFDKKLDSQTDALRAGNESSPEEEFRISDLKAIFTSKTFMVVAGLCVLFYSAIFPFQK
                     FATGMLESRLGMTTSEASGLFSYFPIGAMILTPLLGWFIDNKGKAATMLMLGSLLVIV
                     CHLIFALTPEENFTFAVALGAIIVLGVSFSLVPASLWPSVPKLVDNKVLGSAYSIIFW
                     IQNIGLMLTPVLIGWALKRSNPGIAEQIASGEAVKYNYTVPMLIFAGFGVAALLLAFY
                     LKALDKKKGFGLELPNIVKKDHDATSNPG"
     gene            6836..>7484
                     /locus_tag="PLI30_06245"
     CDS             6836..>7484
                     /locus_tag="PLI30_06245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005680754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HOI79187.1"
                     /translation="MHFQLTSEYRPTGDQPEAIKALIEGLRQKIPYQTLLGVTGSGKT
                     FTIANVIAQLNKPTLVLSHNKTLAAQLYSEFKGFFPNNAVEYFVSYYDYYQPEAYIPT
                     TDTYIEKDLSINDEIEKLRLAATSSLLSGRNDVIVVSSVSCLYGIGNPEDFNKTTVDI
                     SEGQLLERDHFLRLLVNSLYSRDETAEFSRGNFRVKGDTVDVFLAYHDYIVRVIFW"
BASE COUNT         2030 a         1516 c         1897 g         2041 t
ORIGIN      
        1 atggccgtgc aatgcttcgc ggggaatgcg gcacggggaa tgagcttggt ggcgctacat
       61 aacggaggtg gcgtaggtat aggaaaagcc gttaacggtg gattcggaat ggtgtgtgac
      121 ggtagtgaac gcgtggacga aatcctccgc tcagcaatgc tctgggacgt tatgggcggt
      181 gttgcccgcc gttcatgggc acgaaatgca aatgctatga gcaccgtgca ggattttaac
      241 cagtcgtttg ccgatgatta ttacattacc gaaccctacc tggtggacga ggaaataata
      301 aagaatatca caaattacaa acaataattt taaaaagctc ttttcctgag gagcaggagt
      361 cttgatatga acaaaataat ggaatgtgtt cctaatttca gcgagggacg cgacaaagag
      421 aaaattgaaa agatcgtgga gtgtttccgc gcaaaaaaaa atgtaaaact gttggattac
      481 agtaatgacg aagaccataa tcggcttgtt gtgactgtta taggcgaacc cgaaccatta
      541 aaaaaaagcg ttgttgatgc cattggtaaa gcggtagaac ttatcgatat gaaccaacac
      601 agcgggcaac atccgagaat gggtgccgtt gatgtagtcc cttttattcc cattaagaac
      661 gtaactccgg aagaggctgt ttcgttttca aaagaggtag cacagacggt tgccgaacgt
      721 tataacttac ctgttttcct gtacgaaaaa tcagcttcag cacctcatcg tgaaaattta
      781 gcaaacatcc gtaaaggtga atttgaaggg atggcggaga aaataaaaaa agacgactgg
      841 aagcctgatt tcggtcctgc agaacgtcat ccaacggccg gggctgttgc tgtgggtgta
      901 cgtatgcctc tggtggccta taacgtaaac ttaggaaccg acaatcttga aatagctcag
      961 tcaatagcca aaaaagtgcg tttcatcgga ggtggattac gtttctgtaa aggaatgggc
     1021 gtggcgctgg aggaacgagg cataacccag gtttccatga acctgaccga ttataccaaa
     1081 acggccattt acagggctca cgaactggtt cgtatagaag ctaaccgtta cggtgtgcct
     1141 gttattggag ctgaaatcgt aggcttggta cctctggaag cattggtgga tactgccgca
     1201 tattatcttg gccttgagaa tttttcacta aaccaggtac ttgaaacaaa actgatggaa
     1261 taaagcgagg cttacctatg gactggatag aacctattgc cgcagttttt ggcgttttga
     1321 gcgtttggtt tgcccgaaaa gaaaatattt tagtctttcc tttcggaatc ataaacgtcg
     1381 caatttatat ctacatctgt tttgctgccc gattgtatgc gaacgctgga attaacgtag
     1441 tttatctggc ttcgaatgtt tacggatggt atttgtggtc ccgtatcgac ggagaaagcc
     1501 gtaaattaca aattaaacgt aatacttcca aggagaacat gatttcctgg accgcagtgg
     1561 tggcgatcta tgtgtctctg ttttttcttt tgagatatgt taaccggaca gataccggtt
     1621 acattcaatc gtatctgccc tatatcgatt cgtttaatac agcttttttt ttggtagcta
     1681 cccttttgat ggcatttaaa aagctggaaa attggcagtt ttggattata ggtaatattg
     1741 tgtcgatccc catctacgcc tcgcagggat tgtattttac gagcgctcaa tacgctattt
     1801 ttctggtttt agctatatcg ggctggaagg agtggaaaag gaaaataaat tacaaataga
     1861 aaagatgaac aaccttgttg tgaaaaatgc ttcgcaagta gttacctgca gtggatttac
     1921 agggaaaaga ggtgaagata tgtcggacct gcatgtgatt gagaatggaa cagtcgttgt
     1981 tactgacggt gttatctcgc acgtgctgaa agagggtgaa ggtctttccg tagacctgtc
     2041 ggattacgaa gtgattcgtg cggagggtaa ggcattgctt cccgggtttg tagatcctca
     2101 tacgcatttt gtgtttggcg gctaccggga agaggaattt gcgtggagga tgcgtgggga
     2161 tagttatatg gacatcatga accgcggcgg tggtattgta aacactacac gcgctacacg
     2221 cgccgcatcg gaagatgaac tttttatcgc cgggaaacaa cggcttgacg ccatgatgcg
     2281 actcgggatt actaccgtgg aaggtaaaag cggttatgga ctcgataagg aaactgaact
     2341 aaaacaactc cgtgtcatgc agcgcctcaa cgaagaacat ccactggatg ttgtttccac
     2401 gttcatgggc gcacacgcta ctcctgaaga gtggaagggt agggaagatg agtttatcga
     2461 tttcaacata aacgaaatgt tccctttggt ggctcaggaa aaattggcgg aatgcgctga
     2521 tattttctgc gaaaaaaatg tatttaccat cgaacaatcg cgccgctacc tgactgcagc
     2581 acgagaacag ggcttcaaac tgaaaatcca cgccgatgaa attgtaccgt tcggaggagc
     2641 cgagcttgct gctgaacttg gttgcctgtc tgccgaccat ttgctgaaag catcgggtaa
     2701 aggcattcgt gcaatggcaa aggcagaagt ggtttccacg ttgctgccgc ttacggcttt
     2761 ctcgcttcgc gaaaattttg cccgtgcccg gacaatgatt gacaacaatt gtatggttgc
     2821 tttggcaact gatttcaatc cgggtagttc gttcagcgca tctgtgccct tgctgtttgc
     2881 gttggcgtgt atttatatga aaatgtcgcc cgaagaagca gtaacggctt tcacgattaa
     2941 cagtgcggct gctgtaggca gagccgacaa aatcggcagt attgatgtgg gcaaacaagc
     3001 tgacctggtg ctgttgcaat ttccgtcgta caaattcctg ccctatcacg tgggtatgaa
     3061 cattgtagaa aaagttatca agcgtggatg tcttcttgtt gaccgttgaa tatcagattt
     3121 ataaataaat aaaatgaaac tacaggattt aactttaaaa caattcttag aaaaaactgc
     3181 cgggaatgag cctgttcccg gtggtggaag tgtatcggca ttaaacggag ccatatcatc
     3241 gtcattggta caaatgctgg caaacctgac tattggcagg aaaaattaca cggatgtgga
     3301 agagcggatg aaaaaaaatg ccggaagatt tgaagaattc aggatgcatt ttctgaacga
     3361 tatcgatcgc gatagtgaag cttataacct tgttttcgaa gcatttaagc ttccgaaaga
     3421 aacggatgag gaaaaagcat atcgcagcca aaatattcag gaggcgacca agattgccgc
     3481 actcgttccg atggaaattg ccgagcgtgc cttcggaatg cttgacctca ttgttgaaac
     3541 tacccgtaag ggcaacaaaa atgccattac cgacggctgc gtggcaatga tgacctgccg
     3601 gactgccgtc ttgggtgcgc tgctgaacgt acgcatcaac ctgggaggat taaaggatgc
     3661 tggattcgtg caaaagctga cagacgcttg cagccgcata gaaagagaaa cagaggcaaa
     3721 agaaaaagag ttaacggatt gggtaaaaac acaactataa acataaagtg aaaaatattt
     3781 attacatcgg aaaagatgaa cttacgttcg acgaccttga acggatcatc aatgaaaaca
     3841 tcaaactcga attatcgcag gaagccaaag aacgtattca aaaatgccgt gattatctcg
     3901 atcagaaaat gcaaaatcag caggaaccta tttacggaat ctccaccggt tttgggtcgc
     3961 tatgcaaccg cactatttcg aacaatgatt tgcatatgtt gcaggagaat ctggtgaaat
     4021 cacacgcgtg cagtgttggg gaagaagtta agccggtgat tgtaaaactg atgtttctgc
     4081 tcaaagcaca cgcactttca ctgggttaca gcggtgtgca ggtggcaacc gttcaacgga
     4141 tgctcgacct gttcaataac gatattctgc ctgtggtcta cgataaaggc tccctggggg
     4201 catcaggcga cctggcaccg ttagccaacc tgtttctgcc tttgataggc gtgggagatg
     4261 tatattacaa agggcaaaaa cgcgatatcg gtagcgtgct cgacgaattt ggttgggaac
     4321 cccttaaact gaaaagcaag gaagggctgg cattgctaaa cggtacccag tttatgtcgg
     4381 cacacggagt gtttgcggtt atgaaagcac aacgcttgtc gaaacgtgcc gacctgatcg
     4441 cggctatttc gctcgatgcc tacgacgggc acctggagcc ttttgacgaa aagctgcacc
     4501 ttatccgtcc gcacctggga caattagaaa cgtcacagaa tattgtccgg tttctggagg
     4561 gtagtgaatt gatctccagg gaaaagaagc atattcagga cccttactcg tttcgttgta
     4621 ttccgcaagt tcacggtgcc accaaagacg ctatcgcata cgtgaaatcg gtattgttta
     4681 cggaaatcaa ttccgtgacc gataatccta ccatcttccc cgatgatgat caaattatat
     4741 cgggtggaaa ttttcacggt cagccactcg ccattacttt tgatttcttg gcactcgccc
     4801 tcgccgaact cggtaatatt tctgaacgcc gggtagcaca actgattctc gggaaccgcg
     4861 gtctgcccga atttttagtg gctaatccgg gattgaattc cggatttatg attccccaat
     4921 acgtgtcggc ttccatcgta agtcagaaca agatgtattg ctatgcggcc tcgagcgact
     4981 ccattgtgtc gtcaaatgga caggaagacc acgtgagtat gggggctaca tcggctataa
     5041 aattgctgaa agtaatggat aaccttgatc tgattttatc gatagaactg atgaatgctg
     5101 tgcaagcgat ggagtttcgt cgtccgctta aatcgtcgcc tgttattgaa aaaaccatca
     5161 aagaataccg gaaagaggtg ccctttgtag aggaagatat tgtcatgtat aaggagattc
     5221 ataaaaccgt tgcgttttta aaccgattgc acgtggatgc gctgtaaaag aacggtgatt
     5281 ttttttatct ttgcacattc gaaacacttt cataacataa actaaaaatt aaatggtaga
     5341 gaaaattcac agtacgcttc gtgattcttc cgtggcaaga tggaccgcat taatactgct
     5401 ggcttccaca atgtttgtgg cttatatgtt tatcgatgtt ttggcacccc tgagtaccat
     5461 gctcgaaact tcgctgaaat ggacgcccga cacttttggc gcggtcgccg gttcggaata
     5521 cttcctgaat gtatttgcct tcttcctgat atttgccggg attattttag accggattgg
     5581 tgtacggaaa gctgcacttg tctccggttc cctgatggta atcggagcgt ccataaagct
     5641 gtatggtatc agcgattatt ttaatggcgg aggcttcgga tacgatttct tcaactcttt
     5701 ctggaccggg ttccctgcat cggcaaaagt ggcttcggtt ggttttgcta tttttggttg
     5761 tggggtggag atggcgggag ttaccgtttc caaagggatc gtaaaatggt ttgccggaaa
     5821 agaattggca ctggccatgg gcctggagat ggctattgcg cggctgggtg tttttgccgt
     5881 attccgtttg tcgccgtacc tggctgaaaa ggggggcgtt tcactttcgg taggtgtggg
     5941 gactttgttg ctcctgatcg gattgttgac tttcagtgtg tacttcttct tcgataaaaa
     6001 gcttgattcg caaaccgatg ctctcagggc cggtaatgaa tcttctccgg aagaggagtt
     6061 caggatcagc gatttaaaag ctatatttac gtcgaaaacc tttatggttg tagccgggct
     6121 atgcgttctg ttttattcag cgattttccc gtttcagaag tttgctaccg ggatgctcga
     6181 aagccgtttg ggaatgacta cctccgaagc gagcggcttg ttttcctatt ttcctattgg
     6241 agcgatgatt ttaacaccct tgctgggatg gtttattgat aacaagggga aggcagctac
     6301 catgctgatg ttggggtccc tgctagtgat tgtttgccat ttgatttttg cgcttacacc
     6361 ggaggaaaac tttacgtttg ccgtggcgtt aggggctatc attgtactgg gtgtttcgtt
     6421 ctccctggtt cctgcttcgc tgtggccttc agttcctaag ttggtggaca acaaggtttt
     6481 gggatctgct tattccatca tattctggat tcagaacatt ggattaatgc tgactccggt
     6541 tttgataggg tgggcattaa aaagaagtaa cccgggtatt gccgaacaga ttgcatccgg
     6601 cgaagctgta aaatataatt atactgttcc gatgcttatc tttgccggat ttggagttgc
     6661 agcactttta ctggctttct acctaaaagc actggacaag aaaaaaggtt ttgggttaga
     6721 gcttccaaac attgtaaaaa aggatcatga tgcgacaagc aatccgggct gaagtgatcg
     6781 gtaagaaata tagcggcatc aaacaaatgg aggattaagt tgttttataa cgactatgca
     6841 tttccaactt acatccgaat acaggcctac cggcgatcag ccggaagcca taaaggcttt
     6901 gatagaggga ctcagacaga aaatccctta tcagacactg ttgggagtta ccggttcggg
     6961 gaaaacattt accattgcca acgtgatagc acaattgaac aaacccacgt tggtgctgag
     7021 tcacaacaag acactggcag cacaactgta cagtgagttc aaaggctttt tcccgaataa
     7081 tgcggtggag tatttcgtct cttattacga ttattaccag cccgaggctt atattccaac
     7141 tacagacact tacattgaga aagacctgtc cattaacgat gaaattgaaa aattgcgttt
     7201 ggcagccact tcatcgttgc tctcgggacg aaacgacgtg attgttgttt cgtcggtctc
     7261 ctgcctctac ggaatcggaa atccggagga tttcaacaaa acgactgtgg atatcagcga
     7321 gggacaattg ctggaacggg atcatttctt gcggttgctt gtaaacagtt tgtattcgcg
     7381 cgatgaaact gccgagttta gccggggaaa ttttcgcgtg aaaggggata cggtagatgt
     7441 ttttttggca taccacgatt atatcgtacg cgtaatcttc tggg
//
LOCUS       DAOSDR010000124         7481 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrimonas sp. isolate AMR_MDS_1826
            NODE_3621_length_7481_cov_4.338002, whole genome shotgun sequence.
ACCESSION   DAOSDR010000124 DAOSDR010000000
VERSION     DAOSDR010000124.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863355
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrimonas sp. (wastewater metagenome)
  ORGANISM  Petrimonas sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Dysgonomonadaceae; Petrimonas.
REFERENCE   1  (bases 1 to 7481)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7481)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:10:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,720
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 35
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7481
                     /organism="Petrimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3621_length_7481_cov_4.338002"
                     /isolate="AMR_MDS_1826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2023866"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            175..1092
                     /gene="cas7p"
                     /locus_tag="PLI30_06250"
     CDS             175..1092
                     /gene="cas7p"
                     /locus_tag="PLI30_06250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014225625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-PGING CRISPR-associated protein
                     Cas7/Csp1"
                     /protein_id="HOI79188.1"
                     /translation="MEIKGILVTALAPFENHIANGGEKLLGNASSIKRRPDGRVYVSG
                     QMQRHVLFSAIDRINTTDPAKGETYVSNGDGISNLIEKDLRADMGGFMHPSKGDYSGR
                     RTAPLSVTPAVAVGVSEVGRDLLVRIRFDQSEDAKDQALATKEFSEYDLMHMNFFLDI
                     SALSISKAFKYEKSFNLGTKYFKHAGDDERKRRARLYLEATKSMNDYASQARNAVSGE
                     PQKVLIVFDTVLSRKASRYFVAGEQEQKNILEELEARGAKYFIGDDTTDKSVYTAYKE
                     ALAFLAANPLYDPAKGTEVVTFEQAFGGE"
     gene            1085..3340
                     /gene="cas3"
                     /locus_tag="PLI30_06255"
     CDS             1085..3340
                     /gene="cas3"
                     /locus_tag="PLI30_06255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019968532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated helicase Cas3'"
                     /protein_id="HOI79189.1"
                     /translation="MNNFKYPILAKPSGITLEQHTQAVVSEGKLLLEQSPFVVQKYQE
                     RIGKSLAIRLERVCQYHDLGKTTHKWQIACQKDYDTFLQWQSQHGGTFKEYSKTENAG
                     KNIRKAGVRHEIQSLRMVEKKKLPLPQLAAIAAHHGKLGNNFEERWTKEDASNLWGIF
                     KKESNRVIEQYSLEQSAKTQYEFAGPRGLLQLVDHRASAYEEGESIPSFTQFNYQFPH
                     KEKRGVQRLIEQHWKNDLLLVRAPTGAGKTDASLLWAQLQIENKLADRLVIAMPTRFT
                     SNALAINVAESLSDTGLYHSSAWSAKFQEQVDAGILEKREAEKIHEFARLLQTPITVC
                     TIDHLLMALTLTREDHHLIAFNMANSCLVIDEADFYDDFTQANILVLLEILKYWNVPI
                     LLMSASLPESVLPSYQNLGFNIDKIIEDTSDNSRDRFEIKSIKKYTDLEEISDLLDLC
                     VEKGTAIVYANTVDKAVMIYDWFKKRNANPILYHSRFTEPHKKDKEKELIKALGRTAW
                     DNNSAQGIAILTQIGEMSINISADIMISELCPIDRLTQRAGRLCRFDKTKVGELYLLV
                     PQKNDLVYPAPYGEYDRKIKAWTPCAAFVKTSEILDTISYSAERLVSLLNHVYAEQDS
                     FSPKAINNAKNLKEYFSWNWLINPKQVTATDDTDANFWKSRNIAPQDTVFVEKPTSKF
                     FYNYFGFQNWKLNHSLELPVFIIEKFKKLHMIDIEKIWIREEEELIYVFREGFYDNEK
                     GPFLIYDNCII"
     gene            3352..4032
                     /locus_tag="PLI30_06260"
     CDS             3352..4032
                     /locus_tag="PLI30_06260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI79190.1"
                     /translation="MHPYLPQLLSDIEVLINDPPLHLDFEVRRRPKKMPEMAELQFTA
                     STTLETLTGIAVESIPGIMALSEEDCHLLAEAFKRLLATLNFEIMDMHDDFSDDAYIR
                     QIVHHWNDTVQYQPLTGYEMEWCTGDEETCPYGKGCMFCGKQENKAEMTGLAPGVWGF
                     FHDDGTRIDPLMLHIPGLCLDCMLYLDDDEVGNVLCTLTRAEYREGEEFRCYNFMAKG
                     NRQNRFPI"
     gene            4029..4541
                     /gene="cas4"
                     /locus_tag="PLI30_06265"
     CDS             4029..4541
                     /gene="cas4"
                     /locus_tag="PLI30_06265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007575131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated protein Cas4"
                     /protein_id="HOI79191.1"
                     /translation="MRVTGTHFNYFMICHRKLWLFANGIQMEHVSELVEMGKLIHETS
                     YPGRSSKFEEVEIGGIKIDYYDAKSWVIHEIKKSDKIEKAHEMQLKYYIWVLEQYGIG
                     GVSGVLEYPRLRKTEQVYLSNPDREEIPAMISDIEAIVLSDQCPEKVEMKMCKNCSYY
                     DFCWSTEADL"
     gene            4570..4974
                     /locus_tag="PLI30_06270"
     CDS             4570..4974
                     /locus_tag="PLI30_06270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006847234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GxxExxY protein"
                     /protein_id="HOI79192.1"
                     /translation="MHYMPVEYRELTEKIIGAAYEVHNILGPGFAERVYRNALITELK
                     ILGISAKPEYGVTVYYKNEKVGEFSCDIMAEDALIIEIKAVENIEPWHEVQLVNYLNA
                     TKTENGLLINFGRSVSVKRKFRKYKPKEIKNE"
     gene            5338..6342
                     /gene="cas1b"
                     /locus_tag="PLI30_06275"
     CDS             5338..6342
                     /gene="cas1b"
                     /locus_tag="PLI30_06275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004373514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004520 - endodeoxyribonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-B CRISPR-associated endonuclease Cas1b"
                     /protein_id="HOI79193.1"
                     /translation="MKKSYYLFNPGRLSRKDNTLKFVPVGEDGLDGPPRYLPVENVEE
                     FYTFGSLEANSSLYNFLGKHGISVHFFDYYENYTGSFMPREGLLSGRMILKQAEVYSN
                     PAARMEIARKFVTGATYNMVKNLKYYHNRGKDLEEIIHSMEHLSTSIGKCLRIDELMG
                     IEGNIRQYYYEAFNLVISHFEMGKRTKQPPQNEVNALISFGNMMCYSQCLRAIHQTQL
                     NPTISYLHSPGERRYSLALDLAEIFKPLLVDRTIFKVLNKREIQSSDFEQKLNRILLN
                     EKGKKTFVASFEERLNETIQHRALKRSVSYRHLIRLECYKLSKHILGIEEYKPFKAWW
                     "
     gene            6358..6621
                     /gene="cas2"
                     /locus_tag="PLI30_06280"
     CDS             6358..6621
                     /gene="cas2"
                     /locus_tag="PLI30_06280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018964772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas2"
                     /protein_id="HOI79194.1"
                     /translation="MYVILVYDIGEKRVGKMLKLCRKYLNWIQNSVFEGEISEVKLEE
                     LRLKARALMDTESDSLIFFKSRDNNWLYKEIVGKERNSLDNIL"
     repeat_region   6843..7480
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=6843..6868
                     /rpt_unit_seq="ctatcaatcgcaccatctttggaatt"
BASE COUNT         2479 a         1447 c         1584 g         1971 t
ORIGIN      
        1 gcaagtggtg agttacctct tgaaaatgca aagaatctac ttattgcttt ctctcgtttg
       61 atcaacaaag cagagaagca agaagtgcca gttgagttta gcaacgatga ttctgatgaa
      121 attgaaacag atgaccacag tgaagaataa ttaaaccaat aaaatacaaa tacaatggaa
      181 attaaaggaa ttttagttac agctcttgcc ccgttcgaga accatattgc aaacggtggc
      241 gaaaaattgt taggcaacgc gtcttcaata aaaagaaggc cagacggacg agtttatgtg
      301 tcgggacaaa tgcagcgtca tgttctattt tcagcaattg atcgaataaa tacaaccgac
      361 cctgctaaag gagaaactta tgtttcaaat ggagatggta tcagcaactt aatcgaaaag
      421 gacttgcgtg ccgatatggg tggttttatg catccgtcaa agggcgacta ctcaggaaga
      481 aggacggctc cattatctgt aacacctgct gttgctgttg gagtaagcga agttggcaga
      541 gatttacttg ttcggattcg cttcgatcaa tctgaggacg caaaggatca ggcattggct
      601 acaaaagaat ttagcgaata cgatttgatg cacatgaatt tctttttgga tattagtgct
      661 ttgagtatct ccaaagcctt taaatacgaa aagagtttca atcttggaac caagtatttc
      721 aagcatgctg gagatgacga acggaaacgt cgcgcgcggc tttacttgga agctaccaaa
      781 tcgatgaacg actatgccag ccaggcccgc aatgcggtga gtggtgagcc tcaaaaagtt
      841 ttgattgttt tcgatactgt tttaagtcgc aaagcaagcc gatattttgt tgctggcgaa
      901 caggaacaga aaaatatttt agaagaatta gaagccagag gcgcaaagta tttcatcggc
      961 gatgacacaa cagataaaag tgtttacact gcctataaag aagccttggc gtttttagct
     1021 gcaaatccac tttatgatcc tgcaaaaggt acagaagtag taacatttga acaagcattt
     1081 ggaggtgaat aacttcaagt atcccatatt agcaaagcct tccggcatta cccttgagca
     1141 acatactcaa gcagtagtgt cggaaggaaa gctattgttg gaacaatcgc ctttcgttgt
     1201 tcagaaatac caagaacgca ttgggaaaag tttagcgatt cgtttggaac gggtgtgcca
     1261 atatcatgat ttgggaaaga caactcacaa atggcaaatt gcgtgtcaga aagattacga
     1321 cacttttctt caatggcaat ctcaacatgg cgggactttc aaagaatatt caaaaacgga
     1381 aaatgcaggc aagaatattc gtaaggcagg agttcgacac gaaattcaat cgttgcgaat
     1441 ggttgagaag aaaaaattgc cgttaccaca actggcagca attgctgcac atcatggcaa
     1501 attgggaaac aactttgaag agcgttggac taaagaagat gcttcgaacc tttggggtat
     1561 ctttaaaaag gaaagtaatc gggtaattga gcaatactcg ttggaacaaa gtgcaaaaac
     1621 acagtatgaa tttgcaggac cacgaggttt gcttcaattg gttgaccaca gagctagtgc
     1681 gtacgaagaa ggagaatcca taccttcctt tactcaattt aactatcaat ttcctcacaa
     1741 agagaaaaga ggcgttcaaa gacttattga gcaacattgg aagaatgatc tattacttgt
     1801 ccgcgctcca acaggagcgg gaaaaaccga tgcgtcacta ctatgggcgc aattacaaat
     1861 tgaaaacaag cttgcagacc gtttggttat agccatgcca actcgtttta catcgaatgc
     1921 tttggcaatt aatgttgccg aaagcttatc cgatactgga ctttaccatt cgagtgcttg
     1981 gtctgctaag tttcaggaac aagttgatgc tggaatatta gaaaaacgtg aagcagagaa
     2041 aattcacgaa tttgcaagac ttcttcagac tccaataact gtttgtacca ttgaccatct
     2101 tttgatggcc ttgacattga caagggaaga ccatcatttg attgcgttca atatggccaa
     2161 ttcatgtttg gttattgacg aagccgattt ctacgatgat tttacacaag caaatattct
     2221 tgttttactc gaaatcctaa aatactggaa tgtcccaatt ctattaatga gtgcttcatt
     2281 acctgaatcg gttttgcctt cttatcaaaa tcttggtttt aatatcgaca agattattga
     2341 agacacttcg gataattcga gagatcggtt tgaaataaaa tcaatcaaaa aatatactga
     2401 tttagaagag atcagtgatt tacttgattt atgtgttgag aaagggactg caatagttta
     2461 tgcaaacaca gtagataagg cagtgatgat ttatgattgg tttaaaaaga gaaatgcaaa
     2521 tccgattcta taccacagtc gattcactga acctcacaaa aaggataaag aaaaagagtt
     2581 aataaaagct ttaggaagaa cagcatggga taataacagt gcacaaggga tagccattct
     2641 tacccagatc ggcgaaatga gtatcaatat cagtgccgac attatgattt cggaattgtg
     2701 cccgattgac cggttgaccc aacgagcagg tcgtctgtgc cgtttcgaca aaacaaaagt
     2761 tggagaattg tatttgttag tgccacaaaa aaacgatctg gtttatcctg cgccttacgg
     2821 tgaatacgat cgaaagatta aagcttggac accatgcgca gcgtttgtaa aaacatcgga
     2881 aattctcgat accataagct atagtgccga aagacttgta agtctattaa atcatgttta
     2941 tgcagaacaa gactcttttt ctccaaaagc aattaacaat gctaaaaact tgaaagaata
     3001 tttttcttgg aactggttga ttaatccaaa gcaagtcact gcaacagatg atacagatgc
     3061 gaacttctgg aaaagtagaa atattgcgcc acaggatact gtatttgttg aaaaaccaac
     3121 ttcaaaattt ttttacaact attttggttt ccaaaattgg aaacttaatc actcattgga
     3181 actaccagtc ttcataattg aaaaatttaa aaagttacac atgatagaca ttgaaaaaat
     3241 atggattaga gaagaggagg aactcattta tgtatttcgt gaaggttttt atgataatga
     3301 aaagggccca ttcttaatat atgataattg tattatctaa actttgccct catgcacccc
     3361 tacctccccc aactgctgtc cgatatcgaa gtgctgatca acgatccgcc tctgcacttg
     3421 gattttgaag ttcgccggcg cccgaaaaaa atgccggaaa tggcggaact gcagtttact
     3481 gcctctacta ccctcgaaac attgacggga atagccgttg aatccatacc cggaattatg
     3541 gcgctttcgg aagaagactg ccatttgctg gcggaggcat tcaaaaggct gctggcgact
     3601 ctgaactttg aaataatgga tatgcacgac gatttttccg acgatgccta tatccgtcag
     3661 atagttcatc actggaacga tacggttcag tatcagccct taaccggtta tgaaatggaa
     3721 tggtgcaccg gcgatgagga aacctgtcct tacggaaaag gttgcatgtt ttgcggtaaa
     3781 caggaaaata aagccgaaat gacaggtctt gcccctggtg tctggggttt ctttcacgac
     3841 gacggcaccc gcattgaccc gctgatgctc cacatccccg gcttgtgcct tgattgcatg
     3901 ctttacctgg acgatgacga ggtaggcaac gtcctgtgca cactgacacg tgcagagtat
     3961 cgtgaaggtg aagaattcag gtgctataat ttcatggcca aaggcaatcg gcaaaacaga
     4021 tttccaatat gagagtaacc ggtacccact ttaattactt tatgatctgc caccgcaaac
     4081 tctggctttt tgcgaacgga attcaaatgg agcacgtttc ggaattggtg gaaatgggaa
     4141 aactgataca cgaaaccagc tatcccggtc gcagttcaaa atttgaggaa gtggagatcg
     4201 gcggaataaa aatagactac tacgatgcaa aaagctgggt aattcacgaa attaaaaaat
     4261 cggacaagat cgaaaaagcc cacgaaatgc aactcaaata ctacatctgg gtcctggaac
     4321 agtacggcat cggtggcgta agcggagtgc tggaatatcc tcgtctgaga aaaacggaac
     4381 aggtttacct gagcaatccc gaccgggagg aaatacctgc aatgataagc gatatagaag
     4441 caatcgtcct gagcgatcaa tgtcctgaaa aagttgaaat gaaaatgtgc aaaaactgtt
     4501 cttactatga tttttgctgg tcaacggagg cggatcttta gcatatgttt tatcaaaaaa
     4561 taatcaagca tgcattatat gcctgttgaa tatcgtgaat taacagagaa aataatcgga
     4621 gcagcctatg aagtccacaa catcctcgga cctggttttg ctgaaagggt atacagaaat
     4681 gccctgataa cagaattaaa aatactggga atttcagcaa aaccggagta cggggtaacg
     4741 gtatactaca aaaacgaaaa agttggagaa ttcagctgcg acattatggc tgaagacgca
     4801 cttataattg aaatcaaagc cgttgaaaac atagaaccct ggcatgaagt acaattggta
     4861 aactacctga atgcaacaaa aaccgaaaac ggactactca tcaatttcgg aagatccgtc
     4921 tcggtcaaaa gaaaattcag aaagtataaa cccaaagaaa taaaaaatga ataaaactcc
     4981 cagcatcttc ccattcccag ccggatacag gaattcaatc caggcgaaaa aacgtataaa
     5041 tgtatttatt atggctttaa aaagctatcc ctaaaaaatc cctgtgctgt aaaacctgta
     5101 aatcctgtaa atcctgtctc aaacaaaaaa acctgtaaat cctgtaaatc ctgtctcaaa
     5161 caaaaaaatc ctgtaaatcc tgcaaatcct gtctcaaaca aaaaaaaact gtaaatcctg
     5221 taaatcctgt ctcaaacaaa taaaatcctg taaatcctgt aaatcctgtc tcaaacaaat
     5281 aaatcctgta aatcctgtaa atcctgtctc aaacaaaaaa aatcctgtaa atcctgtatg
     5341 aagaaatcct attacctctt caaccccggc cgtttgtcaa gaaaagacaa taccttaaaa
     5401 tttgtacctg tcggcgaaga tgggctggat ggtccgccac gctatcttcc cgttgaaaac
     5461 gtagaagaat tttacacatt tggttctctt gaggccaaca gcagcctgta caattttctg
     5521 ggcaagcatg gcatcagcgt acattttttc gattactacg aaaactacac cggttcattt
     5581 atgccgcgcg aagggctttt gagcgggaga atgattctga agcaggctga ggtttattcg
     5641 aacccggcgg cacgcatgga aatagcgcga aaatttgtta ccggcgcaac gtacaacatg
     5701 gttaagaatc tgaaatacta ccataaccgt ggcaaagatc tggaagaaat catccattcg
     5761 atggaacatt taagcacatc cattggtaaa tgtctccgga ttgacgaact gatgggaatt
     5821 gagggtaaca tcagacaata ttattatgaa gctttcaatc tggtaatcag ccattttgag
     5881 atgggcaagc ggacgaagca gccgcctcag aacgaggtaa atgcattaat atcgtttgga
     5941 aatatgatgt gttattcgca gtgtttaagg gcaatacatc agacacagct caatccgacc
     6001 atcagttatc ttcattcgcc tggagaaagg cgctacagtc tggcactgga cctggcagaa
     6061 atattcaaac cgcttctggt tgaccgtacc atttttaaag tgttgaataa aagggagata
     6121 cagtcaagcg attttgaaca gaagctaaat cgcatactat taaacgaaaa aggaaagaag
     6181 acttttgttg catcctttga agagaggctg aacgaaacga tacagcaccg tgcattgaaa
     6241 aggagtgtga gttaccggca tttaatcagg ctggagtgtt acaaactgag caaacacata
     6301 cttggaattg aggaatacaa accatttaaa gcctggtggt agaaaggagt aacgaccgtg
     6361 tatgtaatat tggtttacga tatcggggaa aagcgggttg gaaaaatgct taaattgtgc
     6421 cgtaaatacc tgaactggat acaaaacagc gtatttgagg gagaaatcag tgaggttaaa
     6481 cttgaagagt tgaggttaaa agcgcgtgca ttgatggaca cggagagcga cagtttaatt
     6541 tttttcaagt cgcgcgataa caactggtta tataaggaaa ttgttggaaa agagcgcaat
     6601 agtttggata acatcctata aaaatcgtcg gtcacgggtt tatgtttgtt cgttatccaa
     6661 attttaagta attttatcga tggaaagaaa cgcaaccctc tgattttcaa aatagggtaa
     6721 aagtagtcga actctggtac aaaaccactt attgttgacc gacgactttt acctacaaaa
     6781 agtgcctgga aagattttat aaagaactga tttacagtta tattttcata gaatcggaac
     6841 ggctatcaat cgcaccatct ttggaattga aactgctttt gttattgtca ttgttggaag
     6901 cgcatcggtc tatcaatcgc accatctttg gaattgaaac ctcaacagcc atcttactcg
     6961 aatttaaaaa tatccgacta tcaatcgcac catctttgga attgaaactt ttaaacacaa
     7021 acggccgtta atgtcccgga tcaactctat caatcgcacc atctttggaa ttgaaactag
     7081 ttttctctgt ttaggcctgg ggagggcggt taatctatca atcgcaccat ctttggaatt
     7141 gaaaccgatg aagagttcga cctgatgaac cagatgatgg gactatcaat cgcaccatct
     7201 ttggaattga aacagcagta caacgacctg agcgccgaac ttgacaagaa actatcaatc
     7261 gcaccatctt tggaattgaa acatgttcag agcatgacaa cggcgcttgc atcagcatct
     7321 atcaatcgca ccatctttgg aattgaaact tggtctatta atgtcccagt atggtgatat
     7381 accctactat caatcgcacc atctttggaa ttgaaactaa atatagcgat tcatctgggg
     7441 tacaagcgca taaactatca atcgcaccat ctttggaatt g
//
LOCUS       DAOSDR010000125         7470 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrimonas sp. isolate AMR_MDS_1826
            NODE_3634_length_7470_cov_3.809305, whole genome shotgun sequence.
ACCESSION   DAOSDR010000125 DAOSDR010000000
VERSION     DAOSDR010000125.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863355
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrimonas sp. (wastewater metagenome)
  ORGANISM  Petrimonas sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Dysgonomonadaceae; Petrimonas.
REFERENCE   1  (bases 1 to 7470)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7470)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:10:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,720
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 35
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7470
                     /organism="Petrimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3634_length_7470_cov_3.809305"
                     /isolate="AMR_MDS_1826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2023866"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..848
                     /locus_tag="PLI30_06285"
     CDS             <1..848
                     /locus_tag="PLI30_06285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005639939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HOI79195.1"
                     /translation="PYYMNTYGFHGIHGRAAAIATGVKVSNPNLNVWVATGDGDSLAI
                     GGNHFIHTVRRNVDINIILFNNKIYGLTKGQYSPTSDRGFVSKSSPFGTVEDPFRPSE
                     LCFGARGTFFARAIDVDMKNTTEIMVAAARHKGTSVVEVLQNCIIFNDGIHNKIADRA
                     WKAEKTIMLKHGEKMIFGLENNRGIVLDGWNLKAVTIGEEGYTMDDILVHDASSTDNA
                     LHIKLSLMGYDNDLPVALGVIRSVDSPSYEAEYENQIVEVQKKAAKKSFTDFLMSSSH
                     IWEMK"
     gene            1085..3805
                     /locus_tag="PLI30_06290"
     CDS             1085..3805
                     /locus_tag="PLI30_06290"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019540989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004591 - oxoglutarate dehydrogenase
                     (succinyl-transferring) activity [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HOI79196.1"
                     /translation="MDTRKELNFMDIGAIEKLYKQYMENPESVDESFRFFFQGFDLAT
                     RHFPAKPQEIKGDVGVSTKEIAVMNLITGYRRRGHLFTKTNPVRSRRQYSPTLDLDNS
                     NLSESDLDKEFEAGKEIGIGRATLREIVEHLSETYCKSIGVEYRYMTKPEIVQWLQQK
                     MESSKNQENFPNEKKLHILNSLIEASGFEDYMHRKYVGQKRFSLEGSEAIIPALDAIV
                     SHGGSYDVNDIVIGMAHRGRLNVLTNIMKKPYSQVFRGFTAENYEEEIKYGDVKYHLG
                     YNNTIDYEGRKISVSLAPNPSHLEAVGPVVEGIAKAKLEQEHDFQYNQVLPILIHGDA
                     AIAGQGVVYETIQFSKLDGYKTGGTIHIVINNQVGFTTNYLQGRSSTYCTDVAKVTQS
                     PVFHVNGDDVEAMVFVAKLALEFRQKFNIDVFIDLLSYRKYGHNEGDEPRFTQPKLYD
                     IIAKHKNPREIYAGKLISQGVLTQLEYETRVADFHKVLDQAYETANESTQLNIQLFLE
                     EKYKGIRFPKSEDFETIANTKISKEMFLELAEKITSLPKDKHFYNKTVRLFSQRAGMI
                     KDDSYDWAMGELMAYASLAKENYPVRLSGQDSERGTFSHRHAEIITEDGEEKYFPLKN
                     LGGEHAMVRVYNSPLNEYGVLGFEYGYSLANPFGLTIWEAQFGDFFNVGQVIVDQYIS
                     AAQEKWGVKSGLVMFLPHGYEGQGAEHSSGRIERFLNLCANFNMQVVNPTTPANHFHA
                     IRRQLHRDIRVPLIAFTPKSLLRHPDCTSTIADFTEGHFQEIIADNSLKSLSKATKLL
                     LCSGKIYYELDAARKEANADHLSIIRIEQLYPFPAKQLQKILKNYSKDIKLVWVQEEP
                     LNMGAALFVKHWIGDGSLQIISRPSSGVTAEGLTALHKIHQAEIIKEAIQ"
     gene            3862..5133
                     /gene="odhB"
                     /locus_tag="PLI30_06295"
     CDS             3862..5133
                     /gene="odhB"
                     /locus_tag="PLI30_06295"
                     /EC_number="2.3.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019540990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045252 - oxoglutarate dehydrogenase
                     complex [Evidence IEA];
                     GO_function: GO:0004149 - dihydrolipoyllysine-residue
                     succinyltransferase activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase complex
                     dihydrolipoyllysine-residue succinyltransferase"
                     /protein_id="HOI79197.1"
                     /translation="MAIIEIRVPSPGESITHVELYKWLVADGAVVKKDTEIAELESEK
                     ATLTLVADESGKISLKASEGDSLEVGSVACTIDTSVQPEADVETEEKKQTTAPADTEK
                     KEIKPEPAKTESRKPEAKATTDDYGNVKVTPLAKRVMEENDLSLEDVISGLRRLKRSD
                     VEAVLAASGVSSVSGLKKPASRESRIDRMSSLRRKLSERLVSVKNETAMLTTFNEVNM
                     KPIMDLRKNYQDRFVEKHGIKLGMMSFFLKACSIALQEFPSVNSMMEGENMTTYDYAD
                     ISVAVSTPKGLMVPVIRNVESMGLSEIEKAIAEVADKARKGKLSIPEMTGGTFTITNG
                     GVFGSMMSTPIINPPQSAILGMHNILDRPVVVEGQIVVRPMMYVALSYDHRVIDGRES
                     VGFLVRVKQLLENPTDMLFGKSSGEKELLEL"
     gene            5280..6026
                     /locus_tag="PLI30_06300"
     CDS             5280..6026
                     /locus_tag="PLI30_06300"
                     /inference="COORDINATES: protein motif:HMM:NF018506.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015144 - carbohydrate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034219 - carbohydrate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GRP family sugar transporter"
                     /protein_id="HOI79198.1"
                     /translation="MVCGISLFFLRISHLNHDFWIYSILGGIAGALGNGFLVKALHLG
                     DLSVLGPINSYKSIVGIIAGIFLLSEIPNIWGILGIALIIYGSYFVLDTTDEKFSWRL
                     LKRPEIQFRIWAMILTAIEAVFIKKVILASSTTVAFMSWCLFGALFSFIVLFFCKLNL
                     KAELSKTMKFNYASKFLLLAGCVGTMQLTTNYTFDHMPVGYALSLFQLSVIISILFGY
                     KFFREKEIGKKIAGSIIMIMGSTLIILLKN"
     gene            complement(6063..7247)
                     /locus_tag="PLI30_06305"
     CDS             complement(6063..7247)
                     /locus_tag="PLI30_06305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019540279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline dehydrogenase family protein"
                     /protein_id="HOI79199.1"
                     /translation="MLLTHNVIDFNNSEIAFRNKSNAELRQAHLLFKVMNNAGLVKIG
                     KHLVNIAFAVHFPVKGILRNTIYQHFVGGTSIADCSKTIQKLAVRNVGTILDFAVEGE
                     ERDELFDATCAEVIRTIEFARNNRHVPFSAFKITGVGRFDLLAKVSEKAQLSEEESKE
                     YRRVYDRVESIFKRGYDLGVPILIDAEHTWIQPVLDDLVLEMMERYNKDVPIVQNTYQ
                     MYRHDAIHRLKDHHRIALDMGVKFGLKIVRGAYMEIERARAAEMGYPSPIQPDKPATD
                     HDFNEVIRYFMDHLDTIHFMVATHNEVSSQLLAQLIDERGLPHDHPHIYFSQLYGMSD
                     HITFNLAEKGYNVVKYVPYGEVKTMMPYLFRRAEENTSVKGQSSRELKLIEKEVRRRK
                     GK"
BASE COUNT         2241 a         1647 c         1704 g         1878 t
ORIGIN      
        1 tgccctacta catgaacacc tacggatttc acgggattca cggccgtgcc gcagccatcg
       61 ccaccggagt gaaagtatcc aaccccaacc tgaatgtttg ggtggcaacc ggcgacggcg
      121 attcactggc aatcggtgga aaccatttta tccacaccgt ccggagaaat gtagacatca
      181 acatcatctt gtttaacaat aagatttacg ggcttaccaa aggacagtac tcccccactt
      241 ccgatcgtgg atttgtatcc aaatcctcgc ctttcggaac agtggaagat cctttccgtc
      301 cgtcagaact ttgtttcgga gcacggggga cattcttcgc cagagccatt gacgtggaca
      361 tgaagaacac caccgagata atggtagccg cagccagaca caaaggaaca tccgtggtgg
      421 aagtattgca gaactgcatc attttcaacg atggtattca caataagatt gctgatcgcg
      481 cgtggaaagc cgagaaaaca attatgctga aacacggcga gaaaatgatt ttcggacttg
      541 agaacaatcg ggggattgtc ctggatggat ggaacttaaa agctgttact atcggcgaag
      601 aaggatatac aatggatgat attctggttc acgatgcctc ctctactgac aatgcattac
      661 acattaagct cagcctgatg ggttacgaca atgatctccc ggtggcgttg ggcgttatcc
      721 gctcagtgga ttcgccatca tacgaagccg aatacgagaa tcagattgtc gaagtacaga
      781 agaaagcagc caaaaagtca tttaccgatt ttctgatgag ctcttcccat atctgggaaa
      841 tgaaataacg aaacaaaagg gcgttgtaga ataaatgggg caatttcaaa aagagattgc
      901 ctcatttttt ttcaggtttt ttcactccga tagaaaattt atcacagcgt atcaacctca
      961 tgtaagggtc gcgttttaca caatcgggtt tgacttaaac cgcttttcta aacaaaattg
     1021 ttgtaaagcg tgaacaaaat catcatcttt acagtttgaa taaattaact acaacaactg
     1081 atcgatggat acccgcaaag agttaaattt tatggatatt ggagccatag aaaaattgta
     1141 taaacagtat atggaaaatc cggaaagtgt cgatgaaagc tttcgttttt tctttcaggg
     1201 ttttgattta gccaccaggc atttccctgc caagccacag gaaataaagg gggatgtcgg
     1261 agtttccacc aaggaaatag ccgtaatgaa cctcattacc ggatacaggc gccggggaca
     1321 cctgtttacc aaaaccaatc cggtccgttc gcgtcgtcaa tattcaccta cactcgatct
     1381 ggataattcc aatctttctg aaagcgatct ggacaaggaa tttgaagccg ggaaagagat
     1441 cggaatcggc agagcaacct tgagagaaat tgtcgaacat ttaagtgaga cctattgtaa
     1501 atccatcggc gtggaatacc gttacatgac gaaaccggaa atcgtgcaat ggttacaaca
     1561 aaaaatggaa agcagcaaaa accaggaaaa cttccccaac gaaaaaaaac tgcacatcct
     1621 caacagcctg atcgaagcat cggggtttga agattatatg cacagaaaat acgtggggca
     1681 aaaacggttt tcgctggaag gctcggaagc tattattcca gccctggacg ccatcgtttc
     1741 tcacggagga tcgtacgacg taaacgatat tgtaatagga atggcccacc ggggaaggct
     1801 caacgtactg acaaatatca tgaaaaagcc gtactcccag gtttttcgcg ggtttaccgc
     1861 tgaaaactac gaagaagaga taaagtacgg cgacgtaaaa tatcacctgg ggtataacaa
     1921 tactatcgat tacgagggac gaaaaatttc cgtcagcctg gcacccaacc cttcccacct
     1981 ggaagcagta ggtcctgtgg tggaaggaat tgccaaagcc aagttggaac aggaacatga
     2041 ttttcaatac aatcaggtgt tacccatttt gatacacggt gatgccgcca ttgccggaca
     2101 aggagttgtt tacgagacca tccagttctc aaaactggac ggatacaaaa caggaggaac
     2161 catccatatc gtaataaaca accaggtggg gttcaccacc aactacctgc agggacgctc
     2221 cagcacctac tgtaccgacg ttgccaaagt aacccagtcg cctgttttcc atgtaaacgg
     2281 agacgacgtg gaagccatgg tctttgtcgc aaagttggca ctggagttcc gtcagaaatt
     2341 caatatcgac gtgtttatcg atctactttc gtaccgaaaa tacgggcaca acgaaggtga
     2401 cgaaccccgt tttacgcaac ccaagttata cgacatcatt gctaaacata agaatccgcg
     2461 tgaaatttat gccgggaaac taatcagcca gggagtactc acccaactgg aatacgaaac
     2521 ccgggtggca gacttccaca aggtgctgga tcaggcatat gaaactgcca acgaaagcac
     2581 gcaattgaat atccaacttt ttcttgaaga aaaatacaag ggtataaggt ttcccaaatc
     2641 agaggatttc gagaccattg ccaataccaa aatatcgaaa gagatgtttc tggaattagc
     2701 cgaaaagata acgtcattgc cgaaggacaa gcacttctac aacaaaaccg tccggctgtt
     2761 ttcacaaaga gccggcatga ttaaagacga ttcatacgat tgggcgatgg gcgaattgat
     2821 ggcttatgca tcgctggcaa aggaaaatta tcccgttcgg ttgagcggac aggattccga
     2881 gcggggaact ttctcgcacc gtcatgctga aatcataacc gaagacggtg aagaaaaata
     2941 ctttccactg aagaacttag gcggtgagca cgccatggtg cgtgtgtaca attcaccact
     3001 caacgaatac ggagtgctgg gttttgaata cggatattca ctcgccaatc ctttcgggtt
     3061 aactatctgg gaagctcagt ttggagactt ttttaacgtg gggcaggtaa tcgtggatca
     3121 atatatttct gccgcacagg aaaagtgggg cgtgaaatcg ggtttggtga tgtttctgcc
     3181 acacggatac gaaggacagg gagccgagca ttcgagcgga agaattgaac gttttttgaa
     3241 cctgtgtgcc aactttaaca tgcaagtggt taaccctaca acgccggcta atcattttca
     3301 tgccatccgc cgccaattac atcgcgatat ccgggttcct ttgatcgcat ttacaccgaa
     3361 aagcttgttg cgccatcccg actgtacatc gaccatcgct gattttaccg aggggcattt
     3421 ccaggaaata attgccgaca acagcctaaa atccctgtcg aaagcaacca aactgctgtt
     3481 gtgttcgggt aaaatctatt acgaactgga tgctgcacgc aaagaagcta atgcagacca
     3541 cctttccatt atccgtattg agcaactcta tcccttcccc gcaaagcaac tccaaaagat
     3601 tctgaaaaat tacagcaaag atataaagct tgtctgggtg caggaagagc cactgaacat
     3661 gggtgctgcc ctgtttgtta aacactggat cggtgacggg agcctgcaga tcatcagccg
     3721 tccttcaagt ggcgtaacag cagaaggctt aacggcttta cacaagatac atcaggcaga
     3781 aattattaag gaagcaatac aataaatgca atctggattc gcttttattc tgaaaaacta
     3841 ttgaataaca accgagatat tatggcaata atagaaataa gggtacccag ccctggagaa
     3901 tcgataacgc acgtcgagct ttataaatgg ctcgttgcag acggagcagt agttaagaaa
     3961 gacacggaaa tagccgagtt ggaatcggaa aaggcaacac tgacattggt agccgacgaa
     4021 tcgggaaaaa tatcgttgaa agcttcggag ggtgatagcc tggaagtcgg ttccgtagca
     4081 tgcaccattg atacgtccgt tcaacccgaa gcagatgtgg aaaccgaaga gaagaaacag
     4141 acaacagctc ccgctgacac ggaaaagaaa gaaatcaaac ccgaacctgc caaaacagaa
     4201 agtcggaagc cggaagcaaa agccacaacg gatgattacg gaaatgtaaa agtgacccca
     4261 ttagccaaac gggtaatgga agaaaacgac ctgtcgttgg aagacgtcat aagtggcctt
     4321 cgccggttga aacgctccga tgtggaagca gtactggcag caagcggcgt aagctccgta
     4381 agcggattaa aaaaaccggc aagccgcgaa tccagaatcg acaggatgag ttcgttacgc
     4441 cgaaagttga gcgaacgcct ggtttccgta aaaaacgaaa cagccatgct tactacgttc
     4501 aacgaagtaa atatgaagcc gattatggat ttgcgaaaaa attatcagga cagattcgtt
     4561 gagaaacacg gaatcaaatt gggtatgatg tcgtttttcc tgaaagcctg ttccatagcg
     4621 ctgcaggaat ttccgagtgt aaactcgatg atggaaggtg aaaacatgac cacttacgac
     4681 tatgccgata tttcggtagc ggtaagcaca cccaaaggac taatggttcc cgtgatccgg
     4741 aacgtggaaa gcatggggct gtccgaaata gaaaaagcca ttgcagaggt tgccgataaa
     4801 gcccgcaagg ggaagttgtc tatccccgaa atgacaggcg gaacgttcac gataactaac
     4861 ggcggtgttt tcgggtcaat gatgtctacc cctatcatta atccccctca atctgccatc
     4921 ttggggatgc acaatatctt ggaccgcccg gtggtggtag aagggcagat tgtcgtacgg
     4981 ccgatgatgt acgtagcact gtcttacgac catcgcgtga ttgacggacg cgagtctgtg
     5041 ggtttcctgg tacgggtaaa gcagctgctc gaaaacccaa ccgatatgct ctttgggaaa
     5101 agcagcggag agaaagagtt gctggaactg tgagtctcaa caagacaaaa aggactccac
     5161 ccgaatggcc gaaacccttt caattacgat agcggtaata ttaaggatat tttccaaccc
     5221 gctggcaaaa agggagccat ccgctggttg ttaactttct cacttatttt ctgctatcgg
     5281 tggtttgtgg catttcgctg ttttttttac gaatatccca tctcaaccac gatttctgga
     5341 tctattcgat actcggaggg atagcaggag cattgggaaa cggatttctg gtaaaggcgc
     5401 ttcacctggg cgatttatcg gtactgggac ccattaattc ctataaatcc attgttggga
     5461 ttattgccgg aatattcctg ctctcagaaa tccctaacat ttggggaatc ctgggcatag
     5521 cattgataat ttacgggagc tactttgttt tagacacgac cgatgagaaa ttctcgtgga
     5581 gattgctgaa acgtcccgag atccaattca gaatttgggc aatgattctt accgctatcg
     5641 aagccgtttt tattaaaaaa gtaatactgg catcatcaac cactgtggca ttcatgagtt
     5701 ggtgcctgtt tggtgctttg ttttcgttta tcgtactgtt tttctgcaaa ctgaacctca
     5761 aagcagaact cagcaagaca atgaaattta attatgcaag taagttcttg ctgttagcag
     5821 gatgcgtagg gacaatgcaa cttacgacaa actacacttt tgaccacatg cctgtcggtt
     5881 atgcattatc gctgtttcag ctttctgtca taataagtat tttgttcgga tataaatttt
     5941 tccgggaaaa agagattgga aagaaaatcg ccggatccat tattatgatc atggggtcaa
     6001 cactgatcat cttacttaaa aattaaaaat cacccttacc cgacaacaaa cctatttcca
     6061 gactatttac cttttcttct tctgacttct ttctcgataa gcttcagttc acgactcgat
     6121 tgtcctttta ccgatgtgtt ttcctcggcc ctgcgaaaaa ggtaaggcat cattgttttc
     6181 acttcaccgt aaggcacgta ttttacgaca ttgtaaccct tctccgccag gttaaaggta
     6241 atgtggtcac tcatgccgta gagttgtgaa aaataaatat gtggatgatc atgaggcagg
     6301 ccgcgttcat caattaactg cgccagcagc tgtgagctca cttcgttatg cgtggccacc
     6361 atgaaatgga tggtatcgag gtggtccatg aaataacgga taacttcgtt gaaatcgtgg
     6421 tccgtagcgg gcttatcggg ctgaataggt gatgggtaac ccatttcggc agcacgtgca
     6481 cgctcaatct ccatatacgc cccccgtaca atcttaagcc caaacttcac tcccatatcc
     6541 aaggcaatcc ggtggtgatc ctttaaccgg tgaatggcat catgacggta catctgatag
     6601 gtgttttgta caatgggcac atctttgttg tatctttcca tcatttccag caccaggtcg
     6661 tccaaaacag gttgaatcca ggtgtgctcc gcatcgataa gtatagggac acccagatca
     6721 taaccccgtt tgaaaattga ctccacacgg tcgtaaaccc ttctatattc tttcgactcc
     6781 tcctcagaaa gctgtgcttt ttcactcact ttggcaagca ggtcgaaccg tccgacaccg
     6841 gttattttaa atgcactgaa cggtacatgc ctgttgttac gggcaaactc tattgtacga
     6901 atgacttctg cacacgtggc atcaaaaagt tcgtcacgtt cctcaccctc aaccgcaaaa
     6961 tcgagaatag tccccacgtt tcgtactgcg agtttttgaa tggttttgga acaatctgca
     7021 atagatgttc cccccacaaa atgctgatag atggtgttac gaaggattcc cttaacgggg
     7081 aagtggacag caaaggcaat gttcaccagg tgtttgccta tctttaccag accggcattg
     7141 ttcattacct taaatagtaa atgagcttgt ctcaattcgg catttgattt atttcggaaa
     7201 gcaatttctg aattattgaa atcgataacg ttgtgtgtca acagcataag ttttctataa
     7261 agtatttaga ttataaatac aaaaataagg atttttttat tcaagaagga ttaaaatgaa
     7321 aaaatcttca tatcaaaatg gcagaaattt atggcgaaat tcgcaggtat tagttagccg
     7381 atttattgaa aagcaaatac tctgtgtcgg aataataaag atgtagttga tttacaaaaa
     7441 ctctgtaatt tgttactctt gaaagacgat
//
LOCUS       DAOSDR010000126         7449 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrimonas sp. isolate AMR_MDS_1826
            NODE_3650_length_7449_cov_3.966324, whole genome shotgun sequence.
ACCESSION   DAOSDR010000126 DAOSDR010000000
VERSION     DAOSDR010000126.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863355
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrimonas sp. (wastewater metagenome)
  ORGANISM  Petrimonas sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Dysgonomonadaceae; Petrimonas.
REFERENCE   1  (bases 1 to 7449)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7449)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:10:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,720
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 35
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7449
                     /organism="Petrimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3650_length_7449_cov_3.966324"
                     /isolate="AMR_MDS_1826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2023866"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(24..2687)
                     /locus_tag="PLI30_06310"
     CDS             complement(24..2687)
                     /locus_tag="PLI30_06310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007655832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI79200.1"
                     /translation="MKTVLFSIFFWTLCFATMLGQNSIKIYPYPMTPSEHPDYSRYLV
                     QSPDASLFENKTEFIALRDLTGNYNEKLDLWINKDKLGKILWVSYPFIYQENIKEIVS
                     EIKRRGLYLFDLWGYVPGSGPGGYWRQFVLPDSISSLFETELGNRWLGMDNGEQDGRY
                     VGSYAPRMYPMGVDRKQQYLNFQRHFEEMGNQLGNKMSTLVSLNFGHYFLKEGVYALI
                     GAETAQGLPNSQIYYSFIRGAGKQYGVSWFGNASVWNRWGFKTYDKDATNIDEDYNSG
                     GPLKGTSLSLLKRLMYNHLMYDCLAVGFEGSMRVDNEKLSPIGKIQQSAAKWVDKYGD
                     PGIMYTPVAVMVDFFSGWTFPRHLYSRQAYKLWGNLPYEKPNYFTDGVLDLLYPGYQD
                     ASYYKDERGFIAPTPYGDIADCLMTDAPLWVLKQYPVLIIADEIQRSSEINDKLETYV
                     KDGGHLVITSASLKNMPNGIAGICVGKKAYTSASEIEYNGDKIQETAEYNLLQLKYPS
                     CAKVIQWSDTIPVTVELNSGKGMITVFASPFGITDQPQCELPALAQEEQVLAKPFPVL
                     NHVKVLLNNIFSSVQLFSANPALSMITCYKGNNEYTVLISNDKWYPQKFHIGTKTGKI
                     ASIKELETDLSEMKAVGFTPKIVRASIGKNTHNTIAGGSVRIFKVNLQDADISVMPKI
                     NPDRPKHKTSLVLRNTLNIKEEILKRPTFFEHYDRVVVDWRYLYNKEKNRLQQEGDWI
                     NRQKLKVSVDLTSGINLFPDLRIVNNDELPYKKSMAIIKNVMDKMSALGADVLLLSTQ
                     QTIENNFTPENFNKSLRETFIELADYAKEKNIQLFLKQVYARTPSSISDLKKLVDDVN
                     RGNFTFSPSLALLLLDEGNIRNSCFALPSNH"
     gene            complement(2801..4342)
                     /locus_tag="PLI30_06315"
     CDS             complement(2801..4342)
                     /locus_tag="PLI30_06315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014224814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HOI79201.1"
                     /translation="MKKLIYFIIIAIGFSSCVDLDLNPNDRPSSGTFWKTPEDINLAL
                     TATYGSMRRNMWSFSTPCWDNLTDNSYGQHAEGQYGLTTNMVQGNIDPSSGGFVSEVY
                     LGALADIARINIFLSKLKDFSGLDSTVKTQYEAEARMLRAYYYSYLYRGYGEVPIVSE
                     PLTLETQYQEKKPQAEVYKFMMDDIDFAIANLKDATYSGAKGRWTRNAAKAYKARMIL
                     YTAYDDAGNMISSKMSEAKTVLSEITGYSLAPDFSDNFIDVKQETSPEIMMSIKFLAP
                     NTTTSADMWYADWLVVSPLLNFMEEFEMKDGTPGTALPKKAGSKFTLDTETFTNASLA
                     NRDPRLAKTVFIEKYVINGTEYTAGNAKPTGAGLAKFLSSNLTPPYGYSTLSQQDWVI
                     MRYADVLLMLAEAENELNGPTLLAYNSINTIRTRAGMPPLPAGLSKDDMRKRIRHERR
                     IELAFEGQRYFDLKRWKTAKTVLNAVQDAPVVYKFEDKNYLWPIPQSEIDKNQGKLSQ
                     NTDYK"
     gene            complement(4355..7435)
                     /locus_tag="PLI30_06320"
     CDS             complement(4355..7435)
                     /locus_tag="PLI30_06320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007652789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOI79202.1"
                     /translation="MKKRHSTTKWKGLLFAMLWTISLGMFAQNITISGNVKDDTGLEV
                     IGATIIVEGNATIGTTTDVDGNYVLNNVPSNGNLVFSYVGMKSQTVAVNGRSRIDVTM
                     ASDTELLDEVIVVGYGTQKKVNLTGAVSAVTGDEMVKRPVTNPATMLQGQMPGVRIIQ
                     GLGQPGNESVQIRVRGQGTYSSAGSDPLVLIDGVPGSLSTLNANDIESVSVLKDAASA
                     SIYGARAANGVVLVTTKSGKDGGFKVNYNANFGVHNPTKMLNIVTNSADYMELTNEAK
                     ANSGISTGLYTQDMINSYRNATDRVKYPNFDWLGYMFNSAFVQNHSLSLNGGNKGTTY
                     NITLGYVDQPGTMKGFTFDKYNFRTNVKSDLKDWITVGVNIAMEKGNQKNPRQSHDDA
                     FLSTLSQAPTYMPFLPDGRYVNSAYNFESHNKNMVAIVENDVMRTTTNYDVSSQLWAD
                     IKLAKGLSWYSKMAVNLGDVSYKDWRPIVNLYDYHSGNFARTLDVGGEGLISHNNRNY
                     YTNFFTYLKYDFNIDQAHNFGLQAGYSQEYNNYQFLQGYRQKYVTNNLQELNAGTAAV
                     QTANGNTTEWALNSYFGRFNYNYMEKYLFEANVRYDGTSRIAKDTRWGFFPSFSAGWR
                     LTEEDFIKNAEINWLTNAKIRASYGLLGNQNIGGDFPYPYQDLLNFTGDYAFDNSVLS
                     PGVAQSALANSLLKWESTSVADAGLDLQLFGKLNITYDWYRKHTFDILRQAQVTGALG
                     LSAPFVNSGDMLNTGHELTVQYNDRINQGALSGLQYGGGFFVDVFRNKLIKFGAREID
                     GYVIRENNLPYNSYYMLDAIGIFQTQEEVDNSPTQFSGNFGPGDIKYRDVNGDNVIDN
                     NDRVTVPGRFPKFEYSFNGNASWKGFDLSFLFQGVEGRRIYTDGWGLEPFIQGAAPTL
                     DFVNNRWTGPGTSNFYPKIYYGWDGTNPNRRASTWYLQDASFLRLKNLTVGYIIPANI
                     TSKVSIDRIRLYFSGDNLLTFTKYKGLDPERAGDGRFVQYPQNKIVSFGVNVEF"
BASE COUNT         1946 a         1602 c         1484 g         2417 t
ORIGIN      
        1 tgcatttccc ttctatttct ttgtcaatgg tttgagggaa gtgcgaaaca tgagtttctt
       61 atatttccct catccaacag caaaagggca agagaaggtg aaaatgtaaa attaccgcga
      121 tttacgtcat caactaattt tttcaagtcc gaaatggatg aaggtgttcg ggcataaact
      181 tgtttcaaaa agagttggat atttttctcc ttcgcataat cagctaattc gatgaaagtt
      241 tctctcaatg atttgttaaa gttttcaggc gtgaaattat tttcaatggt ttgttgggtt
      301 gaaagtaaca aaacgtctgc tcctaaagcg gacattttat ccattacgtt tttaatgatt
      361 gccatacttt ttttataggg tagttcatcg ttattgacaa tacgcaaatc aggaaacagg
      421 ttgattccag aggttaagtc tactgacacc tttaatttct gacggtttat ccaatctcct
      481 tcctgttgta gtctattttt ttctttgttg tataaataac gccaatcgac tacaaccctg
      541 tcgtaatgtt caaagaaagt agggcgtttc aggatctctt cttttatatt cagggtattc
      601 cttaagacca gtgatgtttt atgttttggt ctatccggat taatctttgg catcacggat
      661 atatctgcat cctgaagatt aaccttgaat attctaacac taccgccagc aatcgtgttg
      721 tgcgtgtttt tccctattga ggctcttaca atttttggtg tgaatccaac agccttcatt
      781 tcagataaat cagtttccaa ttcctttatc gatgctattt ttcctgtttt tgtaccgata
      841 tggaactttt gtggatacca tttatcatta gatataagaa ccgtatattc attattgcct
      901 ttataacatg taatcatact taacgcagga tttgcagaaa atagttgaac agatgagaaa
      961 atattgttca acaacacttt tacgtggttt aaaaccggaa aaggtttcgc taaaacctgt
     1021 tcttcctgtg ccagagccgg caattcgcat tgaggctgat cggtaatgcc aaaaggagaa
     1081 gcaaacaccg taatcattcc tttgcctgaa ttcaattcta ctgttactgg aattgtatca
     1141 ctccattgga ttacttttgc gcatgatgga tactttaact gcagcaggtt atattctgct
     1201 gtttcctgta tcttatctcc attgtattct atctccgatg cagatgtata cgctttcttc
     1261 ccgacacaga tgcctgctat accattcggc atatttttta aagatgctga cgtgataaca
     1321 agatggccgc cgtcttttac atacgtttcg agtttgtcat ttatttcaga actccgttgt
     1381 atttcatctg cgataatcaa aacaggatat tgctttaata cccataacgg agcatcggtc
     1441 attaaacagt cagcaatatc tccatacgga gttggagcaa taaaccccct ttcatcttta
     1501 tagtaggaag cgtcttgata tccgggatac aataggtcga gtacaccgtc tgtaaagtag
     1561 ttcggctttt cgtaaggtag atttccccac aatttgtatg cctgacggga ataaagatga
     1621 cggggaaatg tccatccgga aaagaagtct accattacag caaccggcgt gtacattatt
     1681 cccggatcgc cgtatttatc tacccatttg gctgcactct gctgaatttt tccaattgga
     1741 cttaattttt cgttgtcaac ccgcatggat ccttcaaatc ctactgctag gcagtcgtac
     1801 atcagatggt tatacatcag tcttttcagc aggctgagtg atgtcccttt caaggggccg
     1861 ccactgttgt agtcttcatc gatgtttgta gcatccttat cgtatgtttt aaacccccaa
     1921 cggttccata ccgatgcgtt cccaaaccaa ctaacaccat attgttttcc tgctccgcga
     1981 ataaatgaat aataaatttg tgaattaggt aatccttgag cggtttcagc tcctattaag
     2041 gcataaactc cttcttttaa aaaataatgg ccgaagttta acgaaacaag tgtagacatt
     2101 ttatttccga gctgattacc catctcctcg aaatgacgtt gaaagtttaa gtattgttgt
     2161 tttcggtcta cccccatcgg atacatgcgt ggcgcgtaac tcccgacata tctcccgtcc
     2221 tgctcgccgt tgtccattcc caaccacctg ttgccaagtt cagtttcaaa cagggatgaa
     2281 atgctgtctg gcaatacaaa ctgtctccaa taacctccgg gtccggaacc ggggacatat
     2341 ccccaaagat cgaataagta aaggcccctt ctttttattt cacttacaat ttctttgata
     2401 ttctcttgat aaataaaagg atacgacacc cataagattt tacccagttt gtctttattg
     2461 atccacaagt ccaatttttc attataattt cctgttaaat cccttagtgc gataaattcg
     2521 gttttatttt cgaaaagtga agcgtctggc gattgaacaa ggtatcttga ataatcggga
     2581 tgttctgaag gtgtcatggg gtaaggatag atttttatgc tgttttgtcc aagcatggtt
     2641 gcaaaacata atgtccaaaa gaatatggaa aacaagaccg ttttcatttt gtgtgttttt
     2701 aaatgtttca gcgtattttt attaacatca tgatgtggat aaggtaagat tgttaaagat
     2761 gaagagggtg tgttaatgca ccctcttcaa gaaagattat ttacttgtaa tcagtgttct
     2821 gcgagagttt cccctgattt ttatcaattt ctgattgagg tatgggccac aaatagtttt
     2881 tatcttcgaa tttataaaca acaggtgcgt cctgcactgc atttagaaca gttttggctg
     2941 ttttccatct ttttaaatca aagtaacgtt gcccttcaaa agccaactca attctacgtt
     3001 cgtgacgtat tctcttacgc atgtcgtctt ttgacaaacc ggcaggtaac ggcggcattc
     3061 ccgcacgtgt gcggatggta tttatagaat tatacgcaag gagagtgggt ccattcagtt
     3121 cgttttcagc ctcggccagc atcaacaata catctgcata gcgcataatt acccagtctt
     3181 gttggcttaa tgtgctatat ccataaggag gggtgaggtt cgatgataaa aatttagcaa
     3241 gtcctgctcc tgttggcttt gcgtttccag cggtatattc cgtaccgtta atgacatatt
     3301 tctcgatgaa aactgttttc gctaaacgcg ggtcccggtt tgccaaagaa gcgttggtaa
     3361 aagtttcagt atcgagggta aatttacttc ccgctttttt cggaagcgct gtgcccggtg
     3421 ttccgtcttt catctcaaac tcttccatga aatttagtaa cgggctaacg acgagccagt
     3481 cggcatacca catatcggca gatgttgtgg tgttgggagc caggaatttt atcgacatca
     3541 tgatttctgg cgatgtttcc tgttttacat cgatgaagtt gtctgaaaaa tccggagcca
     3601 gggagtagcc tgtaatttcg ctcaacactg tttttgcttc ggacatttta ctgctaatca
     3661 tattgccggc atcatcatag gcggtataaa gaatcatacg cgctttgtag gcttttgctg
     3721 cattccttgt ccatcggcct tttgccccgc tgtatgtggc atcttttaga ttggcaattg
     3781 cgaaatcgat gtcgtccatc ataaatttat atacttcggc ttgaggcttc ttttcctgat
     3841 attgtgtttc gagggtcaac ggttcactta caatcggcac ttctccataa cctctgtaga
     3901 gataactgta gtagtatgcc cgtagcatcc gtgcttcagc ttcgtattgg gttttaacag
     3961 tactgtctaa tccgctgaag tctttcaatt tactcagaaa gatgttgatt cgggcaatat
     4021 ctgcaagtgc tcctaaataa acctctgaaa caaaaccccc cgatgaggga tctatatttc
     4081 cctgtaccat gtttgtcgtt aatccgtact gtccttcggc atgttgcccg taactgttgt
     4141 cggttaagtt gtcccagcat ggagttgaga atgaccacat attgcgacgc atgctgccgt
     4201 aggttgcagt gagggccaga ttaatgtcct ccggggtttt ccaaaaagtt cccgaagaag
     4261 gcctgtcgtt tggatttaaa tcgagatcca cacacgagga aaagcctata gcaattataa
     4321 taaagtaaat taattttttc attgttgttc agttttaaaa ttcaacattc actccaaaag
     4381 atacgatttt gttctgagga tattgaacaa accttccatc tccggcacgt tccggatcca
     4441 atcccttata tttggtaaaa gtaagaagat tgtccccgga gaagtacagc cttattctgt
     4501 caatgcttac cttagaggtg atgttagcag gaattatata cccaacagtg aggttcttta
     4561 gccttaagaa cgaggcgtcc tgcaaatacc aggtactggc tcttctgttg ggatttgtac
     4621 cgtcccatcc ataatagatt ttagggtaga agttggatgt gccgggtcct gtccagcggt
     4681 tattcacaaa atccagcgtg ggtgcagctc cctgtataaa tggctccaat ccccagccgt
     4741 ccgtgtagat tcttcttccc tcgactccct ggaacaggaa tgacaaatcg aatcctttcc
     4801 atgatgcgtt tccgttgaac gagtattcaa attttggaaa tctaccgggt acggttacgc
     4861 ggtcattgtt atcgatgacg ttgtccccgt tcacatcgcg gtacttgata tcaccggggc
     4921 caaaatttcc gctgaactga gtgggcgaat tatccacctc ttcttgtgtc tggaaaatgc
     4981 cgatggcgtc aagcatataa tatgagttgt agggtaaatt gttttcgcga ataacatatc
     5041 catcaatctc tcttgctcca aacttgataa gcttgttcct gaaaacgtct acaaagaagc
     5101 ccccgccata ttgcagtccc gacaaggcac cctggttaat cctgtcattg tactgaacgg
     5161 taagttcatg gcctgtattc agcatgtcac cgctattaac aaatggggcg cttaatccca
     5221 atgccccggt aacctgtgct tgccttaaaa tatcaaaggt gtgttttctg taccagtcat
     5281 acgtaatgtt taattttccg aataattgca agtcgagtcc ggcgtctgca accgaggtcg
     5341 attcccattt cagcaatgag tttgcaagag cggattgagc aaccccagga cttaatacag
     5401 aattgtcaaa ggcataatcg ccggtaaagt taagtaagtc ttgataggga tagggaaaat
     5461 ctccacctat attttgattt cctaaaagtc cgtaagacgc tcttatcttt gcatttgtca
     5521 accaatttat ctctgcattt ttaataaaat cttcttcggt taatctccat cctgcagaga
     5581 acgaagggaa gaagccccaa cgagtgtctt tagctattct tgatgtgccg tcataacgaa
     5641 cattagcttc aaataggtat ttctccatgt agttatagtt aaacctgccg aaataggagt
     5701 tcagcgccca ttctgttgta tttccattgg cggtttggac agcagcagtt cctgcattca
     5761 actcttgaag attgttggtt acatactttt gccgatatcc ctgtagaaac tggtagttgt
     5821 tatattcctg gctatatccc gcctgtaatc caaaattgtg tgcttgatct atgttgaaat
     5881 catatttcag gtaagtaaag aaatttgtgt aataatttcg gttattgtgc gatattaagc
     5941 cttcaccacc cacgtcaagt gtccttgcaa agttgccgga gtgatagtcg taaagattga
     6001 ctatcggacg ccagtcttta taggaaacat cacctagatt tacggccatt ttcgaatacc
     6061 agcttaatcc ttttgctaat ttgatatctg cccatagttg tgatgataca tcgtaattgg
     6121 tcgtggttct cattacatca ttttccacga tggctaccat gtttttgtta tggctttcaa
     6181 agttgtacgc agaattcaca tatcttccat cgggtaaaaa gggcatatat gtgggggctt
     6241 gcgacaaggt tgacaaaaat gcatcatcat ggctttgccg tggatttttt tgatttccct
     6301 tttccatcgc aatatttact ccaacggtga tccaatcttt caaatcagac tttacatttg
     6361 tcctgaagtt gtatttgtca aatgtaaatc ctttcattgt tcccggctga tccacatatc
     6421 caagcgtaat gttatatgtg gttcccttgt ttcctccgtt aagcgataag ctgtgatttt
     6481 gaacaaaagc tgaattgaac atgtatccca gccaatcgaa attcgggtat tttaccctgt
     6541 ccgttgcgtt ccggtatgag ttaatcatat cttgtgtata taaacctgta gatatcccgg
     6601 aattagcttt ggcttcgttg gttaactcca tgtagtcggc ggaattggtg actatattta
     6661 acattttggt cggattatga actccaaaat tggcgttgta gttcacttta aaaccgccgt
     6721 cttttcccga tttagttgta acaagaacta ccccattggc agcacgtgca ccataaatag
     6781 atgcagatgc agcatccttc aatacagaaa cactctctat gtcgtttgcg tttagcgtag
     6841 aaagtgatcc cggaacaccg tcaattaaca ccaaaggatc agaaccggca ctgctgtatg
     6901 taccttgacc gcgaactctt atttgaacgg attcattccc cggctgtcca agtccttgta
     6961 taattcggac tccaggcatt tgtccctgaa gcatggttgc cggattggtg accggccgtt
     7021 ttaccatttc atctccggtg actgccgaaa cggctccggt taaattcacc ttcttttgtg
     7081 taccataccc cacaacgatc acttcgtcca acaattccgt atcggatgcc atagtaacgt
     7141 cgatacggga acgtccgttc accgcaacgg tttgtgattt catccctaca taactgaaca
     7201 ccaggtttcc gtttgaaggt acgttattca aaacgtagtt cccgtcaaca tcagtagtgg
     7261 ttccgatggt agcattacct tccacgataa tagttgctcc gatcacctca agtccggtat
     7321 cgtctttcac gttaccgcta atggtaatgt tttgagcaaa cattcctaag gatattgtcc
     7381 acagcatggc gaacaacagg cctttccatt tagtagttga atgtcttttt ttcattctgt
     7441 tctcatttt
//
LOCUS       DAOSDR010000127         7434 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrimonas sp. isolate AMR_MDS_1826
            NODE_3662_length_7434_cov_4.331481, whole genome shotgun sequence.
ACCESSION   DAOSDR010000127 DAOSDR010000000
VERSION     DAOSDR010000127.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863355
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrimonas sp. (wastewater metagenome)
  ORGANISM  Petrimonas sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Dysgonomonadaceae; Petrimonas.
REFERENCE   1  (bases 1 to 7434)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7434)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:10:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,720
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 35
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7434
                     /organism="Petrimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3662_length_7434_cov_4.331481"
                     /isolate="AMR_MDS_1826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2023866"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            307..594
                     /locus_tag="PLI30_06325"
     CDS             307..594
                     /locus_tag="PLI30_06325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI79203.1"
                     /translation="MKKLLIITLILSIVSVVFMVFNFAASTDIYRDYVGTAIVSGQII
                     DNVGKLPEWTTCKGEWQLLRIDLIVRFIFMLLVTVVLAKLIRSHKVRSNHQ"
     gene            complement(610..1983)
                     /locus_tag="PLI30_06330"
     CDS             complement(610..1983)
                     /locus_tag="PLI30_06330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018336151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HOI79204.1"
                     /translation="MNITKVLRQNNTNDPHLLGRGDIKKLLAQYSLPAIIGMVITSLY
                     HIVDSIFIGHGIGASALSGMAVTFPIMNILAAFSTLIGVGGATLTSIRLGEKDEENAR
                     SILGHVALVNTVNGTLLGVVTLFFLEPVLKLFGASETLLPYASDFLSVYMIGIPVGFV
                     FIGLNNIMRVTGYPKKAMLSSFLTVAVNVLLAPIFIFVLGWGMKGTALATVLSQLAGL
                     VWVLMHFFNKNSLIRFEIKGFKLSGKTVVNMMAIGVSPFLMNLTSSLVVSIINFGLMK
                     HGGDLAIGAYGIINRILFLFGMIVIGLTMGMQPIVGYNYGAKQIDRSFKALRYTIIVS
                     VLIMTFGFLFSQLFPTLIMRMFTSDRELLDISVRGLRITTVLFPVIAVQMVISNFFQS
                     VGKAKIAVFLSLTRQLIFLIPFLLLLPRYFGLDGVFMSMPAADLIAFFVTILTFTYQY
                     KGMRVRK"
     gene            2207..3196
                     /locus_tag="PLI30_06335"
     CDS             2207..3196
                     /locus_tag="PLI30_06335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005646997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HOI79205.1"
                     /translation="MARLVTLYSLQWGDLSLEEVCVKAKAFGYDGLELGLPDHLDVRQ
                     TDPAYYKGIKDLLEKYGMQLRTISSHLVGQAVCDKIDERHKSILPDYIWGDGDPEGVR
                     RRAAEELILTARAARVLGVDTVVGFTGSPIWHLLYSFPPIPQSTIDEGYRDFAHRFTP
                     ILEEFKKLGVRFALEVHPTEIAFDTFSAQRALEALDHHPAFGFNYDPSHLGYQGVDYV
                     DFIYQFPDRIFHVHMKDAYWSDTPKQVGVFGGHVPFGDPRRFWNFRSVGRGKIKFEEI
                     IRALNAIGYQGPLSIEWEDSAMDREHGARESCEYTKRLDFQVSGHAFDAFFAK"
     gene            3216..4385
                     /locus_tag="PLI30_06340"
     CDS             3216..4385
                     /locus_tag="PLI30_06340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008154974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOI79206.1"
                     /translation="MENRRLKMGMVGGGITGFIGAIHLRAALMENMVELACGCFSSNP
                     EVSKESGRTYFLPDHRIYSTYREMFEKETDLPENERMDFVVIVTPNKYHFEPAMMALE
                     RGFHVVLDKPMTFSLEEAKTLQKKVEETGLVLALTHVYSGYPAVKEMKARIAAGELGK
                     LRRVYVEYPQGWLAERIELTEGNNAGWRTDPQLSGKAGCMGDIGTHAWHLCEYVTGLK
                     VVELCAELNTFVPGRPIDDDGVAMMRMEEGVKALLSASQVAVGEANNINIRVYGEKGG
                     IQWVQEHPNQLFLKKGNSPEEVMHIGGNHPYLGKNTRWNIRTPGGHPEGFIEAFANIY
                     RNFALTVKAKMKKAPPSADILDFPDVYDGVRGMQFIETVVESGWSDGPKWIKWRE"
     gene            4436..6328
                     /locus_tag="PLI30_06345"
     CDS             4436..6328
                     /locus_tag="PLI30_06345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004328069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HOI79207.1"
                     /translation="MSKTIISFLNEAVEKYSERPFLYEARTGTDYTSLSYKEVQDKAR
                     QFAAGLMALGIEAGDRVALISEGKNNWVIGELGVLHAGAVCVPLSVKLETEQDISFRV
                     NHSECCAIMASDQQIAKIRPMKGAFSTVRQYILLDPEETLQENETLFNSIIEQGKKLL
                     ANNPSALEERIKAVTPDSLANISYTSGTTANPKGIMLSHGNYVCNSEQAVEHLNGIPS
                     HFRQLLILPWDHSFGHTAGIYSFMKCGASLASVAMGKSPMEVLRNVPKSLKAINPHLL
                     MSVPALASNFRKNIEAGIEKKGKLTSMLFRKALKTAYAYNKEGFNKGSGGRFWIKPLL
                     AFYDKIIFSKVRESFASNMEFFIGGGALLDIELQRFFYAIGIPMYQGYGLSEATPIIS
                     ANCPHAHKLGSSGKPLPHMDIKIVDDKMQVVPTGKKGEIIIRGGNVMKGYWKNPEATA
                     ETIVDGWLRTGDMGYIDNDGYLYVLGRTKSLLISDDGEKFSPEGIEESIMAQSPFINQ
                     IVLYNNQRPYTTALITVNPVELKKRTSDSSEAALLIEKEIAEYLKGGKNDGMFPYRWL
                     PSAFAVIEEPFTEKNGMVNSTMKIVKHKVYSAYASRIEELYTPAGKKSTSPANLEVLG
                     RLLKEN"
     gene            complement(6345..6506)
                     /locus_tag="PLI30_06350"
     CDS             complement(6345..6506)
                     /locus_tag="PLI30_06350"
                     /inference="COORDINATES: protein motif:HMM:NF024081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FeoB-associated Cys-rich membrane protein"
                     /protein_id="HOI79208.1"
                     /translation="MSLQLFIVIIIGIVVSIVVVREIYRFFFVKKESGFCGGCKGCDF
                     NPGHIQKTK"
     gene            complement(6537..>7434)
                     /locus_tag="PLI30_06355"
     CDS             complement(6537..>7434)
                     /locus_tag="PLI30_06355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009017843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ferrous iron transporter B"
                     /protein_id="HOI79209.1"
                     /translation="RTIESRSSRMITMLIVPFMSCSARLPVYILFVSAFFPRNGSFVM
                     LGLYSVGVLVAILTAILLRKRFFTKEDTPFVMELPPYRMPTFKSVVIHMWERAKQYLL
                     KMGGPILVASIIIWFLGYFPQDVQREAQFDRQLAQLDSLVLPQTEKEIRVIEIEHAKN
                     TEHQVNSYIGKIGHFMEPLMRPLGFDWKISVSLLSGMAAKEIVISTMGVLYTGDSEDQ
                     QSLQTRLLQEKHPDGTPVFSPLVVIGFLLFILIYFPCIATIVAIKEESHSWRWALFSV
                     VYSTGLAWFIAFLVYQTGRFFI"
BASE COUNT         2241 a         1702 c         1713 g         1778 t
ORIGIN      
        1 catcaaaaag tatcggggta cggtactggt tatttcccat gatgcatatt tcatcaatga
       61 gattggggta acaaagagca ttcaattagg aacaaaacaa aaataatttt tttatacaga
      121 tacaaaaaaa tacactactt ttactttttt tagaaacagt aggcacataa atagaaaaga
      181 atccgtgatg atgtaaacaa accccgacgt ttatgtcgag gctatccctt ggtaatgctc
      241 aggtaaatcc ttgcccgagt catcttacgt acaaataaat accccaatta tcattatgta
      301 aagaagatga aaaagttatt gatcattaca cttatcttaa gcattgtttc cgttgtattt
      361 atggttttca attttgccgc ttcaacggat atttatcgcg attatgttgg aacagcaatt
      421 gtttcaggac agatcatcga taatgtcggt aaattgcccg aatggacaac ctgtaagggt
      481 gaatggcagt tattacggat agatttaatt gttcggttca ttttcatgct actggttacc
      541 gtggttctgg caaaattgat aagaagccac aaggttaggt caaatcacca gtaaggaagt
      601 taatcccttt tatttcctaa ccctcattcc tttatactga taggtaaacg tcaggattgt
      661 tacaaaaaac gcgattaaat cagcggcagg catactcata aaaactccat ccaaaccaaa
      721 ataccggggg agcaaaagta aaaagggaat cagaaaaata agctgccgcg tcaacgatag
      781 gaacacggct atttttgctt ttccaaccga ctggaaaaag ttactgataa ccatctgaac
      841 ggcaataaca ggaaacaaaa cagtggtaat tctcaatccg cggacagaga tgtcaagaag
      901 ctccctgtcg ctggtaaaca tgcgcatgat caaagttggg aatagttggg aaaataaaaa
      961 accgaaagtc atgattaaaa cacttacgat gatcgtgtac ctgagcgcct tgaacgacct
     1021 gtcgatttgt tttgccccgt agttgtatcc tacgattggc tgcataccca ttgttaaacc
     1081 aataacaatc atcccgaaca ggaaaagtat ccggttgatg attccgtatg ccccgatagc
     1141 aagatcacca ccatgtttca tcaatccgaa attgataatc gaaacaacaa ggctggaggt
     1201 gagattcata agaaaaggag aaacaccgat agccatcatg ttgacgaccg tctttccgga
     1261 caatttaaac ccctttatct caaaacgaat aaggctgttc ttgttgaaaa agtgcatcag
     1321 cacccacacc aatcccgcaa gctgggagag gactgtggcc agcgctgtcc ctttcatgcc
     1381 ccagccaaga acaaaaatga aaattggcgc aagtaaaaca tttactgcaa ctgtcaggaa
     1441 agaagagagc attgcctttt taggatatcc ggtcacacgc ataatgttat tcagcccgat
     1501 aaagacaaaa ccaacaggaa ttcctatcat gtaaaccgac aagaaatcac ttgcgtaagg
     1561 caacaacgtt tcactagctc caaaaagctt taaaacaggt tccaggaaaa acagcgtcac
     1621 taccccaagc aatgtcccgt tcactgtatt taccaaggca acatgtccaa gtatacttcg
     1681 tgcgttctct tcgtcttttt cacctaatct gatagaggtt agtgttgcac ctcccacacc
     1741 aataagggtg gaaaaagcgg caagaatatt cataatcgga aaagtcacag ccatgcccga
     1801 aagtgcggat gcaccgatac cgtgcccaat aaaaatactg tccactatat ggtaaagcga
     1861 tgtgataacc ataccgataa tagcgggtaa agagtattgt gcgagaagtt ttttgatgtc
     1921 gccccttccc aaaaggtgcg gatcgttagt gttgttttgg cgtaacacct ttgttatgtt
     1981 cataacccgg gcacaaaggt acaaaattta tgtcaagctc gtttggggat ttagatttct
     2041 taagagttaa gtaacgcaca aaattaaatt tttccgggaa accccgagaa ccatccttgt
     2101 ttttcttgaa aaaaagaagt agttttgcgt ggaatcaagt cagtctacga acccggcaaa
     2161 cacttatcct atagtatttt cttaaatcat aaaaaacaga ataaacatgg cacgattagt
     2221 tacactctat tccctgcaat ggggcgattt atcactggaa gaggtttgcg taaaggcaaa
     2281 agcattcggt tacgacggac ttgaattagg attacccgac cacctggatg tccgtcagac
     2341 cgatcctgcc tattacaagg gcattaaaga ccttttggaa aaatacggca tgcaattgcg
     2401 aaccatctcc tcccacttgg tagggcaagc tgtgtgcgat aaaattgacg aacgccacaa
     2461 aagtatcctc cccgattata tctggggaga cggcgatccg gaaggagtcc gccgacgtgc
     2521 cgctgaagaa ttaatcctga cggcccgggc agcccgggtg ctgggggttg acaccgtcgt
     2581 agggttcacc ggaagtccga tatggcattt gttgtattct ttccctccta ttccacaatc
     2641 aactatcgat gaaggatacc gggattttgc acaccgtttt acccctattt tggaagaatt
     2701 taaaaagttg ggcgtacgtt ttgcattaga ggtacatccc accgaaattg ctttcgacac
     2761 cttttctgcc caaagggcat tggaggcctt ggatcatcac cccgctttcg ggttcaacta
     2821 cgatccgagc catttgggat accagggggt ggattacgtg gattttatct atcagtttcc
     2881 cgaccgtatt tttcacgtac acatgaaaga tgcctactgg agtgacactc ccaaacaagt
     2941 aggagtattc ggaggacacg tccctttcgg cgacccccgt cgcttttgga acttccgcag
     3001 tgtagggcgc gggaaaataa aattcgaaga gattatacgt gcccttaatg ccataggata
     3061 ccagggcccg ctttcgatag agtgggaaga cagtgctatg gaccgcgagc atggagcccg
     3121 tgaatcgtgt gagtacacca agcggctgga ctttcaggta tccggacatg cgtttgatgc
     3181 tttttttgcc aaataatcaa caaaaataca gtgtaatgga gaacagacga ttaaaaatgg
     3241 gaatggtcgg aggcggtata accggcttta tcggagccat tcaccttcgc gcagccttaa
     3301 tggagaatat ggtagaattg gcctgcggat gtttcagctc aaacccggag gtatcgaaag
     3361 aatcgggacg cacttatttc ctgccggacc atcgtattta cagcacctac cgggagatgt
     3421 ttgagaaaga aacggacctg ccggaaaacg aaaggatgga tttcgtggtg atcgtcaccc
     3481 ccaacaaata ccatttcgaa ccggctatga tggccttgga aaggggcttt cacgtggtac
     3541 tggataaacc catgacattc tcccttgaag aagccaaaac acttcaaaaa aaagtggaag
     3601 aaaccggttt ggtactggct ttgactcacg tttactccgg atatcccgcg gtaaaagaga
     3661 tgaaggcgag aattgccgca ggagagctgg gcaagttacg ccgtgtatac gtagaatacc
     3721 ctcaaggctg gctggcagaa cgtatcgagc taacggaagg gaacaatgcg ggatggcgta
     3781 ccgatccaca attatcgggg aaggcaggtt gtatgggtga tataggaacc catgcctggc
     3841 atttgtgcga atacgtaacc ggattaaaag tggtggaact ttgcgccgaa ctgaatactt
     3901 tcgttcccgg gcgtccgatt gatgacgacg gagtggccat gatgcggatg gaagaagggg
     3961 taaaagccct gctttcggcc agtcaggttg ccgtgggtga agccaacaac atcaacatcc
     4021 gtgtctatgg ggaaaaggga ggaatacaat gggttcagga gcatcccaac cagcttttcc
     4081 taaaaaaagg gaacagcccc gaggaagtga tgcatatcgg cgggaaccat ccttacctgg
     4141 gtaaaaatac ccggtggaac atacgtacac ccggagggca tcccgaaggt tttatcgaag
     4201 ctttcgccaa tatctaccgt aacttcgcgc tgacggtgaa ggcaaaaatg aaaaaagccc
     4261 ccccctctgc cgatatactg gattttccgg acgtgtacga cggtgtacgg ggtatgcaat
     4321 ttattgaaac agtcgtggag tcaggatgga gcgacggccc aaaatggata aagtggaggg
     4381 agtagtattc cataaaaaac agtaaatttg cagcaaaaaa ttaaatccaa catcgatgag
     4441 caaaacaatc atctcttttt tgaacgaagc tgttgagaag tacagcgaac gtccgtttct
     4501 ttacgaagca agaacaggga cagattatac ctcactctcc tacaaggaag tccaggacaa
     4561 agccaggcag ttcgctgccg ggttaatggc gttggggatc gaggcgggcg accgtgtcgc
     4621 cctgatttcc gaaggaaaaa acaactgggt gataggtgag cttggggtgc tgcatgcggg
     4681 ggctgtctgc gtccctcttt ccgtgaaact ggaaacggag caagacatca gttttcgcgt
     4741 gaatcattcc gaatgttgcg cgattatggc ttccgatcag caaatagcga aaatccgccc
     4801 gatgaaggga gccttttcta ctgtcaggca gtacatcctg cttgacccgg aagaaacctt
     4861 gcaggaaaat gaaactttgt ttaactccat aatcgaacag ggaaagaagt tgctggccaa
     4921 caatccgtca gcgttggaag aacggataaa agccgttact cccgattcac tggccaacat
     4981 ttcttacacc tcgggaacaa cagctaaccc gaaagggatt atgttgtcac acggcaacta
     5041 cgtttgtaac tcggaacagg cagtggagca tttaaacgga ataccttccc atttcaggca
     5101 actacttatc cttccgtggg atcattcctt cggacacacc gccggaattt attcgttcat
     5161 gaaatgcgga gcttcactgg catcggtagc tatgggtaaa tcccctatgg aggttctccg
     5221 gaacgtaccc aaaagcctga aagcgattaa ccctcatctg ctgatgagcg tacccgcact
     5281 ggcttccaac ttccgaaaaa acatcgaagc cggaattgaa aagaaaggca aactgaccag
     5341 tatgttattc cggaaagccc ttaaaacagc atatgcgtat aacaaagaag gcttcaacaa
     5401 aggctccggt ggacgtttct ggataaaacc gcttctggcc ttttacgata aaatcatctt
     5461 ttcaaaggta cgtgaatcgt ttgccagcaa tatggaattc ttcattgggg gtggcgccct
     5521 cctcgatatt gaactgcaac gcttcttcta tgcaataggc attcctatgt accaaggcta
     5581 cggcctatca gaagcgactc ccatcatctc cgccaattgc cctcatgcac acaaactcgg
     5641 ttcttcaggg aaaccgcttc cgcatatgga tataaaaatt gtggacgaca agatgcaggt
     5701 agttcccacg gggaagaagg gtgagatcat tatccggggc gggaacgtga tgaaaggata
     5761 ttggaaaaac cccgaagcca cggccgagac cattgtggat ggatggctcc ggacaggaga
     5821 tatgggatac atcgacaacg acggatacct gtatgtgctt gggcgcacaa aatcgctgct
     5881 gatcagcgat gacggtgaga aattttctcc cgaaggaatt gaagagtcca tcatggccca
     5941 gtctccgttt atcaatcaga ttgtgttgta caacaaccaa cgcccctata ccacagcgct
     6001 tatcaccgta aatcccgttg aattaaaaaa gcgaacgtca gactcctcgg aagcggctct
     6061 acttattgaa aaggaaattg cggaatacct caagggagga aagaacgacg ggatgttccc
     6121 ttaccggtgg ctgccttccg catttgccgt gatcgaagaa ccctttaccg aaaagaacgg
     6181 catggtgaac tccacgatga aaatcgtaaa acacaaggtc tattcggcat acgcatcgcg
     6241 cattgaagaa ttatacacac ctgcagggaa aaaatcaaca tcaccggcca acctggaagt
     6301 actgggaaga ctgttgaaag agaattaaat ggtttgattc tcctttattt tgttttctgg
     6361 atatgcccgg gattaaaatc gcatcccttg cacccgccac aaaacccgga ctccttcttc
     6421 acaaaaaaga aacggtaaat ctcacgaaca accacgatgg agacaacaat gccgatgatg
     6481 ataacgatga acaactgtaa actcatattt taacaacaaa gaattggacg gaaagtttaa
     6541 ataaagaaac ggccggtttg ataaaccagg aaagctataa accaggccag ccctgtcgag
     6601 tagaccacgc tgaaaagcgc ccacctccac gaatgggatt cttctttgat agcaactatg
     6661 gtggcaatac aggggaaata gatcaggata aacagcaaaa atccgataac gaccagcggt
     6721 gagaaaacag gtgtcccgtc cggatgcttc tcctgtaaaa gccgggtttg caacgactgc
     6781 tggtcctcac tgtcaccggt ataaagcacc cccatcgtac tgattacaat ctcttttgcc
     6841 gccattcccg acaacaagct cacggaaatt ttccagtcga aaccaagcgg gcgcatcaat
     6901 ggctccataa aatggccaat ttttccgatg tatgaattta cctgatgttc ggtgttcttt
     6961 gcgtgttcaa tttctatcac ccggatctct ttttctgtct gcggtaaaac caggctgtcg
     7021 agttgtgcca gttgcctgtc aaattgcgct tcgcgctgta catcttgcgg gaaatacccc
     7081 agaaaccaga taatgatgga ggcaaccaaa atgggaccgc ccatcttcag caaatactgc
     7141 ttggcccttt cccacatatg gatgactacc gatttaaatg taggcatccg gtaaggcggc
     7201 aactccatca caaacggcgt atcttccttg gtaaaaaatc gtttacggag caaaatcgcc
     7261 gtcagaatag ccacaagaac ccctacgctg tacaatccga gcatgacaaa actgccgttc
     7321 cggggaaaaa atgcacttac aaataaaata taaaccggca atcgcgcact gcacgacata
     7381 aaaggaacaa tcagcatggt aatcatccgg ctgctgcggc tttcgattgt gcgg
//
LOCUS       DAOSDR010000128         7369 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrimonas sp. isolate AMR_MDS_1826
            NODE_3699_length_7369_cov_3.797375, whole genome shotgun sequence.
ACCESSION   DAOSDR010000128 DAOSDR010000000
VERSION     DAOSDR010000128.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863355
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrimonas sp. (wastewater metagenome)
  ORGANISM  Petrimonas sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Dysgonomonadaceae; Petrimonas.
REFERENCE   1  (bases 1 to 7369)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7369)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:10:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,720
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 35
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7369
                     /organism="Petrimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3699_length_7369_cov_3.797375"
                     /isolate="AMR_MDS_1826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2023866"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1316)
                     /locus_tag="PLI30_06360"
     CDS             complement(<1..1316)
                     /locus_tag="PLI30_06360"
                     /inference="COORDINATES: protein motif:HMM:NF024627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HOI79210.1"
                     /translation="MRNLSRRYYLFLFLLYFLGSCAGNNRVYVSVDGNDKGTGKRNDP
                     FMTLERARDYIREKRWGETDDRPYTILLRGGNYRIERTIHFTDKDYNITIQSFPDEKV
                     TLTGSILIAPEQILPVAGTDKEEIFPEANRNKVFMVRLKDLPIEDYGQLEPVGFGRPV
                     TPAPMELFINGKPGHLSRWPNDSSVNIGKVIYSGSLADDKRQEGATFTYPGDRPYGWK
                     HPEKVWIAGYFNHGWADDAIRLASIDSSKKAITTFHPHRYGFGSGKPWNQWYAYNIVE
                     ETDSPGEYCIDREEGILYFFDPGTLSTLEVSLLEEPIMEMLGASDITVKGIVFENARG
                     SAAALYGTGNCIFSGCIFRNLGSFAISIEDATGFSQQPQQAFSSNNGIVDCIIYETGR
                     GGIVLSGGDRNTLTPARNYVHNCTIHDFNRIAKTYSAGIKISGVGN"
     gene            complement(1322..2071)
                     /locus_tag="PLI30_06365"
     CDS             complement(1322..2071)
                     /locus_tag="PLI30_06365"
                     /inference="COORDINATES: protein motif:HMM:NF012516.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004650 - polygalacturonase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 28 protein"
                     /protein_id="HOI79211.1"
                     /translation="MSKKIVITCVALILTLSMFAKDYKASLFDIKSDGVTLNTASIQY
                     AIDYISANGGGQLNFYVGRYLTGSFHLKPNVTIQLHEGAVLVAFQSIYDYVSVNNTQA
                     LILADNVENIGITGKGVIEGHGQGVLKSITDQVENGHLEKSALQTRPALIHFNGCSNI
                     KLEGLILRDACGDVQTYSGCKNININNITVESKAVPGSKGMVISNCDSVTLSNSYFDT
                     TGNEIDTNQASRNVSVKETINSKGKKLQSKR"
     gene            complement(2111..3598)
                     /locus_tag="PLI30_06370"
     CDS             complement(2111..3598)
                     /locus_tag="PLI30_06370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005645601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004650 - polygalacturonase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 28 protein"
                     /protein_id="HOI79212.1"
                     /translation="MSKINIYFSIVFILLATHLQAKDYNVSDFGAKADGTTVNTRTIQ
                     RAIDYVSENGGGRLVFQVGNYVTGSIYLKSNVTLHLEPGATILGSTNPLDYVIDPKIK
                     WSSMIFAVNQENIGITGKGTINGRGFTTANNLVSLIHRGIFEDELLLDRPREWRRPEN
                     IYFRECVNVTITGITLRDPASWNQTYDQCKNVYVDGIYVDSKAYWNNDGIDIVDCDGV
                     TIKNSYFDAADDVICFKSHDANSICQNVVVENCVGRSSANGIKFGTVSRGGFRNFKIK
                     NVTIFDTFRSAITFAAVDGGIIEDIEVDSIRSINTGNVIFLRIGDRWSSGKQPSMKNV
                     RISNVYAEVPMSKPDAGYNYEGPVEDNPRNISPAVIFGLPDHKIEDVVLKNIEIVYPG
                     AGNPLYAYRGTSQTELDSIPDMRERYPEFSQWKELPAWGFYVRHADGVVFDNVRLVAE
                     KKDYRPSIVIDNVDGATFRNVEFIEPESKNKKQIIQNRSKNVTIQ"
     gene            complement(3638..5116)
                     /locus_tag="PLI30_06375"
     CDS             complement(3638..5116)
                     /locus_tag="PLI30_06375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009133034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004650 - polygalacturonase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 28 protein"
                     /protein_id="HOI79213.1"
                     /translation="MKRIKTVFLTLAVFSILHLSAAEYKASYFGIKSNGTTLNTTSIQ
                     KAIDYIHEKGGGTLVFYVGRYLTGTIELKSNVHIVLREGAILVGSTNIYDYNIEEPIF
                     MPALVYANKASNISITGKGVIDGQGAEVAYNLVDQVHKGIVEDKLQLDRPATRRPGGI
                     YFRECNHVSISGITIKNTAFWVQTYDQCENLRIDGITVDSKVFWNNDGLDIVDCKDVV
                     VSNSFIDASDDAICFKSHDKTKRCENIEVRNCVARSSANGIKFGTVTSGGYKNIRIIN
                     NKVYDTFRSAFTIATPDGGKVEDIFVDSLYAYNTGNAIFLRIGTRWNKDKQGTIDNVT
                     IQNMYAEITATKPDAGYGYEGPIEDLPRNVSPAVIYGIPGVDINNVTLRNIEIVYPGG
                     SNPNYAYRGTKPADLDSIPELIDSYPEFSNWKELPAWGLYVRHASNLTLENVTFTAKE
                     KDYRPAVVFDDVKGATLSRMQYNVPGRGKDLIRHKSTRIIQK"
     gene            complement(5238..>6692)
                     /locus_tag="PLI30_06380"
     CDS             complement(5238..>6692)
                     /locus_tag="PLI30_06380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HOI79214.1"
                     /translation="ELLKIGSDQAIMSGITHSIWHGFNYSPPEAPFPGWVQYGSYYNE
                     NNNWWPYFKYLNRYKARISSLLQHADMYTDIAILPANYDMWGEMGVQTDPFPEKLNIP
                     YTSIIWESINKNGGAADYVTEKIIQEAKIEKGKLCYGPKKYGILFLPEVKSMFPDTLA
                     KLYEFVRGGGKIFAIECLPEKSLGLHNYKENDKKVSAWTDKLRAYSDRFVLTPKPDDL
                     RYLEWYTGLQQKYQLPHYLEISRPDRYLMQTRYQADDKSEIFFFSNAHKHENRDTRIT
                     FSKEITRGRNPWILDPETGKSYRLKLDGNSLQLNLGPAESRIIVFNKERKGEYWEELP
                     LNHPAAQFLNNNWEVELRHSREKWVKSTQMETLQDLKDTEHVRFTGTVVYKKRIQIHK
                     KGAILNLGKVSGVSELFVNGKSQGVKWYGNRIYKLGDDVKTGENEIEVHVITTMGNYM
                     QALTDNPTAQKYTNRKGREQEIQSMGLVGPVTVY"
     assembly_gap    6693..6702
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<6703..>7369)
                     /locus_tag="PLI30_06385"
     CDS             complement(<6703..>7369)
                     /locus_tag="PLI30_06385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009133031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HOI79215.1"
                     /translation="GAAGPILNHMDKEAVKKYLNHMSDTIQKKTGPLSNHLRAFFTDS
                     MELEGCNWSADLADEFGKRRGYDLMPYLPFTMFKVGRLGAVVDENYGVKKSPEFEEII
                     RRVRFDFEYTKAELLYERFTQTYLEWCREQNVKSRAQAYGRGFFPLETSLGYDIPEGE
                     SWTTNWLRHKLGEEMSDEDYRRGRGYTMINKYVSSAAHLTGKRLISCEEMTNTYLVFN
                     ATL"
BASE COUNT         1681 a         1730 c         1717 g         2231 t
ORIGIN      
        1 tgatttccca ctcccgatat tttaatccct gcactgtagg ttttggcgat gcgattgaaa
       61 tcgtgaatgg tgcaattgtg gacataattg cgggcggggg taagggtatt acggtctcct
      121 cctgaaagaa caattccacc tctccccgtt tcatagatta tacaatcgac gatgccgttg
      181 ttgctgctga atgcctgttg tggctgttgt gaaaaaccgg ttgcatcttc aatggatatg
      241 gcgaaactgc ctaaattcct gaatatacaa cctgaaaaaa tacaattgcc ggttccatac
      301 aatgctgccg cacttcctct tgcattttca aaaacgattc ctttaacggt gatatcggat
      361 gcgcccagca tctccataat aggctcttcg aggagcgaaa cttccagggt agatagtgtt
      421 cccggatcaa aaaaatagag gattccctct tcccgatcga tacagtattc acccggggaa
      481 tctgtttctt ccacgatatt gtaggcatac cattgattcc agggttttcc cgatccaaaa
      541 ccgtacctgt gtggatgaaa agtggtaatg gcctttttag atgaatcaat cgacgccagc
      601 cttatagcat catccgccca tccgtgattg aaataaccgg caatccatac tttctccgga
      661 tgcttccatc cgtaaggcct gtcccccgga taggtaaagg ttgccccttc ctgtcttttg
      721 tcatcagcca gggacccgct gtagattacc tttccgatgt taaccgatga atcgtttggc
      781 catcgggaga ggtgtcccgg ttttccgttg ataaataact ccatgggggc cggtgttaca
      841 ggccggccaa agccgaccgg ctctaactgg ccataatcct cgataggtaa gtctttcagg
      901 cggaccataa ataccttgtt cctgtttgct tcgggaaaga tctcctcttt atctgtccct
      961 gcaaccggaa gaatctgttc cggtgcaata agaatactgc ctgttaatgt gactttttca
     1021 tcggggaagg attgtatggt aatgttgtaa tctttgtcgg tgaagtggat cgttcgctcg
     1081 atgcggtagt tgcctccacg cagcaagatg gtgtacgggc gatcgtccgt ttctccccat
     1141 cttttctccc tgatataatc cctggccctc tccaaggtca taaagggatc gtttcgttta
     1201 ccggtgcctt tgtcattccc gtctacagag acatataccc tgttgttgcc tgcacatgat
     1261 cccagaaaat acaacaggaa taaaaagagg taatatcgtc tggataagtt cctcactgtt
     1321 tttatctttt ggattgaagc tttttacctt tggaattgat ggtttccttt accgacacat
     1381 ttcttgatgc ctggtttgtg tctatctcat tgcctgtggt gtcgaaatag gaattgctga
     1441 gggttacgct gtcacagttg gaaattacca tccctttgct tccgggcact gccttgctct
     1501 ctactgtaat gttgttaatg ttgatgtttt tacagccgga ataggtttgt acatctccgc
     1561 aggcatcccg caggataagt ccttccagtt tgatgttact gcatccgttg aaatgaatca
     1621 atgccggtct ggtttgtaat gcactttttt cgaggtgtcc gttctctacc tgatcagtaa
     1681 tgcttttcaa taccccttgt ccatgtcctt caatgactcc tttaccggtg atgccaatat
     1741 tctcaacgtt atccgccagg ataagtgcct gggtgttgtt gacactaacg taatcataaa
     1801 ttgactgaaa agcgaccaat acagcacctt catgcagttg gatcgttacg ttgggcttta
     1861 agtggaaact gcctgtgagg tagcgtccta catagaagtt gagttgtccg cctccattgg
     1921 cactgatata atcaatggcg tattgtatgg atgctgtatt taaggtgacg ccatctgatt
     1981 tgatgtcaaa cagggatgct ttgtaatcct ttgcgaacat tgagagtgtg aggattaaag
     2041 cgacgcaggt aataactatt tttttgctca ttttgttttg tcttttaatg gttgaatggg
     2101 gtaaacagca ttattggatc gttacatttt tggaacggtt ttgaataatc tgctttttgt
     2161 ttttgctttc gggttcaata aactctacgt tgcggaaagt agctccgtcc acattgtcga
     2221 ttactatcga aggacgatag tcttttttct cggcaaccag tcttacgttg tcgaatacca
     2281 caccgtcggc atgacgtaca tagaatcccc atgcgggcag ttctttccat tgtgagaatt
     2341 cagggtatct ttcacgcatg tcggggatac tgtccagttc agtctggctg gtgccgcgat
     2401 atgcatataa cggattgccg gcacccggat atacaatttc gatgttcttc aatactacat
     2461 cctcgatttt atggtcggga agcccgaata ttacggcggg agagatgttg cgcggattat
     2521 cttccaccgg gccctcgtag ttataacccg cgtcgggttt tgacatcggt acttctgcgt
     2581 atacattgga aatgcggaca ttcttcatac tgggttgctt gccgctgctc cagcggtcac
     2641 ctatacgtag gaaaataacg ttgccggtgt tgatggagcg gatgctgtcc acctcaatat
     2701 cctcgattat tcctccgtct accgcagcaa aagtgatggc agaacgaaac gtgtcaaata
     2761 tggtcacgtt cttaatttta aagttcctga atcccccgcg ggatacggtg ccaaacttga
     2821 ttccatttgc gctggaacgg cctacacagt tctccactac cacattctgg cagatactgt
     2881 tagcatcgtg cgatttgaaa cagatgacat cgtccgccgc atcaaaatag gagtttttaa
     2941 tggttacccc gtcacaatcg acgatgtcga ttccgtcgtt gttccagtat gctttgctat
     3001 ctacatagat accatctaca tacacattct tacactggtc gtaggtctgg ttccagctgg
     3061 ccgggtcgcg aagagtgata ccggtgatgg tgacgttgac acactcccgg aagtagatat
     3121 tctccggccg tctccactcc ctgggcctgt cgagcaagag ctcatcttca aaaatgccgc
     3181 ggtggatcag gctgaccagg ttgttggccg tagtgaatcc ccgtccgttt atggtccctt
     3241 taccggtaat tccgatgttc tcctgattca cggcaaaaat catggaggac cacttgattt
     3301 tggggtcaat cacataatcc agcggattgg tggatcccag tatggtcgca cccggttcca
     3361 gatgaagggt cacgttcgat ttcaggtaaa tgcttcccgt cacgtaattg cctacctgaa
     3421 aaacgaggcg gccgcctccg ttttcactta cgtaatcgat ggctctttgt atggttcttg
     3481 tgttaaccgt tgttccgtcg gcttttgctc caaagtcgga tacattgtaa tcttttgcct
     3541 ggagatgagt ggcaagaagt atgaacacga tcgaaaaata tatatttatc ttactcatga
     3601 ttgttgttgc tgattgatga ataaatctga tttaagctta tttttgaatg atacgggttg
     3661 atttatgacg gatgagatct tttcctctgc ccggaacatt gtattgcatt ctggacaatg
     3721 tagctccttt tacgtcgtca aacacaacgg cgggacggta atctttctct ttggcagtga
     3781 aagtcacatt ctccaaggtg agattgctgg cgtgacgtac atagagcccc catgccggca
     3841 attccttcca gttggaaaat tccggatagg agtcaatcaa ttccgggata ctgtccaggt
     3901 ctgcgggttt agtaccgcga taggcatagt tcggattgct gccgccgggg tacacaattt
     3961 cgatattgcg cagggtcacg ttgttgatgt ctactcccgg gataccgtat atgacggcgg
     4021 gagatacgtt gcggggaagg tcttcgatcg gtccttcgta tccgtagccg gcgtccggtt
     4081 tggtggcagt aatttccgca tacatgttct ggatggtaac attatcaatg gttccttgtt
     4141 tgtctttatt ccagcgtgtt ccgatacgta agaaaatagc attcccggta ttataagcgt
     4201 aaaggctgtc cacaaagatg tcttccacct ttcctccatc gggagtggca atagtaaaag
     4261 cggaacggaa agtgtcgtat actttgttgt tgatgatgcg gatgttcttg tatccgcccg
     4321 aagtgaccgt cccgaatttg atgccgttgg cacttgaacg ggctacgcag ttacgcacct
     4381 cgatattctc acatcgtttg gttttatcgt gcgatttgaa acagatggca tcgtccgaag
     4441 catcgataaa agagttgctc actactacgt ccttgcagtc gacaatatcc agcccgtcat
     4501 tgttccagaa tactttgctg tcgaccgtga tcccgtcaat tcgtaaattc tcgcactgat
     4561 cgtacgtttg aacccaaaaa gcggtgttct taatggtaat tccgctaatg gatacatggt
     4621 tacactcccg gaaataaata ccgcctggcc gtctggttgc aggacggtct aattgtaact
     4681 tgtcctcaac aattcccttg tggacctggt caaccaagtt gtaagccacc tcggctccct
     4741 gcccgtcaat cacacccttc ccggttatgg agatgttgct tgccttgttg gcgtacacca
     4801 atgccggcat aaaaatgggt tcttcgatgt tgtaatcgta tatgttggtt gatcctacca
     4861 gtatggcccc ttcccggagc acaatatgca cattcgattt caattcgatg gtgccggtca
     4921 ggtagcgccc cacatagaaa accagtgtgc cgcctccctt ttcgtggatg taatcgatag
     4981 ccttctgaat agaggtggtg tttaacgtgg tgccgttgga cttaatacca aagtatgatg
     5041 ctttgtattc agcggctgat agatgcaaaa tagaaaatac cgcaagtgtc agaaaaacag
     5101 tttttattct tttcatttgt ttatatttta tgttttatgt tttacgttag gctacatgaa
     5161 actgtccctt tatgccatgg agaggaatag cctaacctta gtgaattcgc tttaatcacg
     5221 tatttatttg ctaatgatta ataaactgta acaggtccaa ccagtcccat cgattggatt
     5281 tcctgttccc tgcctttccg gttcgtgtat ttctgggcag tggggttgtc agttaatgcc
     5341 tgcatataat tacccatggt tgtgatgaca tgcacctcga tttcattctc tccggttttt
     5401 acatcatcac ccagcttgta aatacggtta ccgtaccatt ttactccttg tgatttccca
     5461 ttgacaaaca gttcggatac accggatacc tttcccagat tcagaatggc tcctttctta
     5521 tgaatctgga ttcgtttctt gtaaaccaca gtgccggtga aacggacatg ctcggtatct
     5581 tttaaatcct gtaatgtttc catttgtgtg ctcttcaccc acttctcccg gctgtggcgg
     5641 agctccacct cccagttgtt gtttaggaac tgtgcggccg ggtgattcaa gggcagctct
     5701 tcccaatatt cacctttccg ctctttgttg aagacaatga tgcgggactc tgccggcccc
     5761 aggtttaatt gcaggctatt gccatcaagc ttcaaccgat acgatttacc cgtctcggga
     5821 tccagtatcc agggattacg tccccgggta atttctttgg agaaagtgat ccgggtgtct
     5881 ctgttttcat gcttgtgggc attggagaag aagaagatct cacttttgtc atctgcctga
     5941 taacgagtct gcatcagata acggtcgggc cggctgattt ccaggtaatg gggcagttgg
     6001 tatttttgct gtaagccggt ataccactcc aggtagcgga gatcatccgg tttgggagtc
     6061 aggacaaagc ggtcggaata tgcacgaagt ttatccgtcc aggcgcttac ctttttgtcg
     6121 ttctccttgt aattgtgcag gcctaatgat ttctctggta ggcactcaat agcgaatatt
     6181 tttcctcccc cgcggacaaa ctcgtagagc ttggccaagg tgtcgggaaa catactcttc
     6241 acctccggca aaaacaaaat gccgtacttt ttgggtccgt agcagagttt tcctttttca
     6301 atttttgcct cttgaatgat tttttcggtt acataatcgg ctgctcctcc attcttattg
     6361 atagattccc agatgatgga agtgtagggg atattcagtt tttcggggaa ggggtctgtc
     6421 tgtacaccca tctcacccca catgtcgtag ttggccggca gtatggctat atcggtatac
     6481 atgtcggcat gctgtaacaa agatgagata cgggccttgt agcgattcag gtacttgaaa
     6541 tagggccacc agttgttgtt ttcgttatag taggagccgt attgtaccca ccccgggaag
     6601 ggtgcctccg ggggtgaata gttgaatccg tgccatatgg aatgggtaat ccccgacatg
     6661 attgcctgat ccgaacctat ttttagtaat tcnnnnnnnn nntccaatgt agcattaaaa
     6721 acaaggtaag tgttggtcat ctcttcacag ctgatcaatc gtttgccggt aaggtgtgct
     6781 gcggaggaaa catacttgtt gatcatggta taaccacgcc cccggcgata atcctcatcc
     6841 gacatctctt ctccgagctt gtggcggagc cagttggtgg tccacgattc tccttccggg
     6901 atgtcgtacc ccaagcttgt ctccaacggg aagaatcccc tgccgtaggc ctgtgccctt
     6961 gattttacgt tttgttcacg acaccactcc aggtaggttt gggtaaaacg ttcatacagc
     7021 aattcggcct ttgtatattc aaagtcaaag cggacacgac ggattatctc ttcaaactcc
     7081 ggacttttct ttacgccata attctcatcc accacagccc ccagccgtcc gaccttgaac
     7141 atggtaaaag ggagataggg catcaggtcg taacctcttc tctttccgaa ctcgtctgcg
     7201 agatcggcac tccagttgca gccttccaat tccatcgaat cggtgaagaa ggccctcagg
     7261 tggttggaga gtgggccggt ttttttctgg atggtgtcgg acatatgatt caggtacttt
     7321 tttacggctt ccttgtccat atggttcagg ataggccctg cagcaccgg
//
LOCUS       DAOSDR010000129         7327 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrimonas sp. isolate AMR_MDS_1826
            NODE_3717_length_7327_cov_3.545792, whole genome shotgun sequence.
ACCESSION   DAOSDR010000129 DAOSDR010000000
VERSION     DAOSDR010000129.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863355
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrimonas sp. (wastewater metagenome)
  ORGANISM  Petrimonas sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Dysgonomonadaceae; Petrimonas.
REFERENCE   1  (bases 1 to 7327)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7327)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:10:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,720
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 35
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7327
                     /organism="Petrimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3717_length_7327_cov_3.545792"
                     /isolate="AMR_MDS_1826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2023866"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..277
                     /locus_tag="PLI30_06390"
     CDS             <1..277
                     /locus_tag="PLI30_06390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI79216.1"
                     /translation="LYWELGEKSLFKQAVRMGNFKAVRYGLKNKVQIYDISKDRKEQN
                     DIATSNPELVAKALELFSSMRHEDLNFPMKDNAKTGRNTKSIDPDDQ"
     gene            281..1801
                     /locus_tag="PLI30_06395"
     CDS             281..1801
                     /locus_tag="PLI30_06395"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HOI79217.1"
                     /translation="MITKFPYSLIGVSLLAGAATQNVFSADKPHCPEHPNLLFIWTDQ
                     QIDNSLAVYGNTVFKVPNLNKFAEESIVFANSYVVQPVSTPSRGSVLTGMYPHMHGAI
                     QNNTPINADAKTLPELINNDMYATGYFGKWHLGDEIFAQHGFEEFDSVEDLYYEYYSA
                     DKDKNARSGYHKFLVENGLEPDDKKNNVFTRNYTASLSYEYSKPKFLEYKAKDFLTRH
                     QNQPFILYVNYLEPHTPFNGPFNELHDMDSLELSQAVDCMLTDNDPVRYHARVRNNVA
                     KEQYRKLYRNYAGLCHAVDLSIGKILKHLDSLGLRNNTIVVFTSDHGEMMGAHGLNNK
                     SVMYEQALRVPLMFRIPWINMNKQRIVTPNVTNIDLVPTLLELMNCQADMSHLQGRSL
                     MEMFNGDRRTNDDGYIYMMWNPNEDDKKSNPVDGYSKEQIAYYTDASFRTVISPDGWK
                     LTLSDRDKNQLFNLNSDPHECNNLYYSGKYSNEIKKLSAKIRRWQKEINDKIKLNN"
     gene            1811..4930
                     /locus_tag="PLI30_06400"
     CDS             1811..4930
                     /locus_tag="PLI30_06400"
                     /inference="COORDINATES: protein motif:HMM:NF014851.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HOI79218.1"
                     /translation="MKRLLTVLLAILAGQVLAKKYTQPKSPRVEHTINRDWTFNYYPS
                     GKENTDISAVDFDDSKWKAIAVPHTWQTYETTKDVHPFIMSASEREDPYWWNGWGWYR
                     KRFSVDSAMKDKKIFIEFDGVQKYCKVFLNGNYIGDHKGGYNSFYFDLTPHIKFGKEN
                     ILAVEVSGRRDDKFGTIAPATAGNFNVYSGIYRDVRLVITNQAYVPYQGSYKHEGGTH
                     ITTPIVSEKEAVVAVKTYVKNESDNDLLIEVKNIIADADNKPVATLKTSKQVNKGELA
                     CFDQKSEYIHNPKLWSNVSPYVYSVYTEVYANGVLTDTYRSPLGFRYFKWDYADNTLI
                     VNGQKIHIHGTNRHQEYPWLGDAIPKWLTEMDMNDIRYNLAHNFMRTGHYPNDPVVYD
                     FNDRNGIITVEEVPNIKPINFDDQVQEQNLREMIRRDRNHPSIFFWSVGNETKDAADS
                     KWAVEEDTTRYIHERKTEGYGDYVNHNATNLDMENLLRVTIRGWYNKDVRDLEPGNGT
                     NVEKSGQQAGTEEWQHKMARVDGGSIRGRIDKNIVAWLYADHGADRIYKDSPLKNVNA
                     KGWTDLYRMPKYMYYLWQANYLERPMIFIHPHHWRAQYLGQKKNIQVDSNCDVVELFV
                     NGKSIGKQHPSKENFHTVEYTDVAVEKGIIKAIGTKDGKKTEYQISMAGNPAKIVLKS
                     SHAAIPADRSGLAIITADIVDAKGVHVYGATNTLKWEVIGDGSLVGHHTYGTDINKNS
                     DVSGCGYIDPPVSNVVRSSNKAGTITIKVSAEGLQDGTITLSTYKSAKPATGITEPEL
                     SDEGRMVTMRDTSFNEVVEYVVEMKRIVSPEQIKASDSVEYRKVMTRFVVERNPGIDK
                     KSIEFEKLIKRLTIYIENVNGELTEDDYNFIARTFNDTRMLAKIIETKNFHPDYAKAL
                     REYYSHAMLVEGKMVDVSKESEKINQIPRELDIVVYVTPEETKINNGLEYERTKYYNR
                     SNISDLDEIITILHPEYAKMNDQVKTKVKKYLVQINPMLIGNDGKYRIAEQSCIAIPK
                     KMINQIKTN"
     gene            4932..>7327
                     /locus_tag="PLI30_06405"
     CDS             4932..>7327
                     /locus_tag="PLI30_06405"
                     /inference="COORDINATES: protein motif:HMM:TIGR04056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0071702 - organic substance transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SusC/RagA family TonB-linked outer membrane
                     protein"
                     /protein_id="HOI79219.1"
                     /translation="MKQKILLRRMIRTLLLMFSVNLFAIWNVTAQDHKVSGIVTDSKS
                     GEPIIGATVYVEGTSNGNITGADGKYELTIDTNSKLSISFVGYIKQTIEVNGRTQIDI
                     ALVADNQEMEEVVVVGYGVVKKRDLTGAISNVKSDQIVKMATSGVTGALVGKVAGLQI
                     TNTSGGPGDGNNVVRIRGGNSISGSNDPLWVIDGFISAGGASSTPAEDIESVEILKDA
                     SATAIYGARGANGVIIVTTKRGKAGATNVTVRSNVGVNWMSRELPLMNGPQTYDYWNA
                     FDKNYLDSRIDPKANFDWINAVTRNSLKQTHYIAVSGGKEGYAYNVSAEYAADEGIIK
                     YNSDTKRLNVRAAFDFDLSKKVKMGITTRYRYSEDNTVGDGDFFQMILRMSPLVAPFK
                     EDGSYNFNLNHGNLSDALGVDLYSKVKGNPIQYVRESLNPSRGGLFNGQAFLNYEPIK
                     GLIFRTEFSYNKNNKWNQSYKPSTIDVMTPAKLQHSEGESYEWVNTATYKKDFGQHSI
                     NTVVGQTLQSDINSFLSGSNKYFAADGFTWNNLGQGNPIEIGDIGVGSGYKSTSILSY
                     LARVNYVYNDKYLFTVSGRADGASKFSKNNKWGYFPSGAVSWRASEEDFIEKMDLFST
                     LKFRASVGITGSEAIKPYETLGLMSGGTILISSSIIPPYGTPTQIFISDKLANDNLTW
                     ESTVQTDFGIDMGFFKNKLSLALDIYNKDTRDLLLYVPVLPESGYDVKLSNVGKVNNK
                     GIEFSINAIPVTSKNFEWSTNLNLSRNKNKVVSLGGASKIDQSSGDMPETTYLEVGRP
                     L"
BASE COUNT         2383 a         1447 c         1653 g         1844 t
ORIGIN      
        1 attatactgg gaacttggcg aaaaaagcct cttcaagcag gcagtaagaa tgggcaactt
       61 taaagccgtt cgatacgggc ttaagaataa agtgcagata tacgacattt caaaagaccg
      121 taaagaacaa aacgacatag ccacgtctaa tcctgagctt gtagccaaag cattagagtt
      181 attctcatct atgaggcatg aagatctgaa cttcccgatg aaggataacg caaaaacagg
      241 cagaaacaca aaatccatag accctgacga tcagtaaaat atgattacaa aatttccata
      301 tagccttatt ggcgtatctt tactagcagg cgcggcaacg cagaatgttt tttctgcgga
      361 taaaccacat tgtcctgagc atcccaattt gttatttatc tggacagacc agcagataga
      421 caactcactg gcagtatatg gcaatacggt ttttaaagtt ccgaatctga acaaatttgc
      481 agaagagagt atcgtttttg cgaattcata tgtagtacaa cccgtttcga ccccatcacg
      541 cggatcagtt cttactggta tgtatcctca tatgcatggg gcaattcaga acaacacccc
      601 gattaacgct gatgccaaaa cccttccgga gctgattaat aacgacatgt atgctaccgg
      661 atatttcggt aaatggcacc ttggcgatga gattttcgcg caacacggtt ttgaagagtt
      721 tgactcggtt gaggatctgt actatgaata ctattctgcc gataaggata agaatgcccg
      781 ttcggggtat cacaaattcc ttgttgaaaa tggtcttgaa cctgatgaca agaaaaacaa
      841 tgtttttacg cgtaactaca ccgcgtcact gtcatatgag tacagcaaac caaaatttct
      901 tgagtataaa gctaaggact tccttacgag gcatcagaac cagccattta tactatatgt
      961 gaattatctg gagccgcaca caccgttcaa cggaccgttc aatgagctgc acgatatgga
     1021 ctctttagaa ttgtcgcaag cggttgattg tatgctaacg gataatgatc ctgtgcgtta
     1081 ccatgcgcga gtgagaaata atgttgcaaa ggagcaatac cggaaactat accgcaacta
     1141 cgccgggtta tgtcacgctg tcgatttgag tatcggcaaa attctgaagc atctggattc
     1201 attgggactt agaaataata ctattgttgt atttacaagc gaccacggcg agatgatggg
     1261 agcacatggt ctcaacaaca aatcagtgat gtatgagcag gcattgcgtg taccgttgat
     1321 gtttcgcata ccgtggataa atatgaacaa acaacgtata gtaacgccta atgtgaccaa
     1381 tatcgacctt gttcccacac ttcttgaact gatgaactgc caggcagaca tgtctcatct
     1441 acaagggcgg tctcttatgg aaatgtttaa tggtgataga cgcacaaatg atgacgggta
     1501 tatatatatg atgtggaatc ctaatgagga tgacaaaaaa agcaatccgg ttgatgggta
     1561 tagcaaagaa caaattgcct attatactga tgcttcgttc cggacggtca tctctcctga
     1621 tggatggaaa ctcactctga gcgataggga taaaaaccag ttgttcaact taaattcaga
     1681 tccacatgag tgcaacaatc tctattattc aggaaaatac agtaacgaga tcaaaaaact
     1741 ctctgccaaa atccgaagat ggcagaaaga gataaatgac aagataaaac ttaataacta
     1801 acgatataag atgaaaagat tactaacagt gctattggct attctggcag gtcaggtgct
     1861 ggcaaagaag tatactcaac ctaagagtcc gagggttgag catacgataa atcgcgactg
     1921 gacattcaac tactaccctt cagggaaaga aaacactgac atttcggccg tggacttcga
     1981 tgactctaaa tggaaagcta ttgctgttcc gcatacatgg caaacttatg aaactaccaa
     2041 agatgttcat ccatttatca tgtcggcttc tgagcgtgaa gacccctatt ggtggaatgg
     2101 atgggggtgg tatcgaaaaa gattttcggt cgacagcgca atgaaagaca agaaaatctt
     2161 tatcgaattt gacggcgttc agaaatattg taaagtattc ctgaatggca attatattgg
     2221 cgatcataag ggcggataca atagtttcta tttcgacctt actccgcaca ttaagttcgg
     2281 aaaggagaac attcttgctg ttgaagtatc aggtcggcgt gatgataaat tcggcaccat
     2341 tgctcctgca actgcaggaa atttcaacgt atacagtggt atataccggg atgttaggct
     2401 ggtgattacc aatcaggcgt atgtgccata tcagggttca tataagcatg aaggtggcac
     2461 gcatatcacg acgcctattg ttagtgaaaa agaagcggtt gtggccgtaa aaacctatgt
     2521 aaaaaacgaa tcagacaatg atctccttat cgaggttaaa aacatcattg ctgatgcaga
     2581 caataagccc gttgcaactc tgaagaccag caagcaggtc aataagggtg aacttgcctg
     2641 tttcgatcag aagagtgagt acatccataa ccctaaactg tggagcaacg tatctccgta
     2701 tgtatacagt gtatacacgg aggtatatgc aaacggcgta ttgaccgata catatcggag
     2761 tcctcttggt ttcaggtatt tcaaatggga ctacgcggac aatacgctta tcgttaacgg
     2821 ccaaaaaatc cacatacacg gtactaatcg tcatcaggag tatccatggt taggagatgc
     2881 aattcctaaa tggctcaccg aaatggatat gaatgatatt cgatacaacc ttgctcataa
     2941 ctttatgcgt acaggtcact atccaaacga tccggtggtt tacgacttta atgaccgcaa
     3001 cggaataatt accgtggaag aagttcccaa tatcaagcct ataaactttg atgatcaggt
     3061 acaggaacaa aatctgcgtg aaatgatccg tcgcgacaga aatcatccgt caatattttt
     3121 ttggagcgta ggaaatgaga cgaaagatgc cgccgattca aaatgggctg tcgaagaaga
     3181 tacgacacgg tatattcacg aaagaaaaac tgaaggttat ggcgactacg tgaatcataa
     3241 tgcaactaat cttgatatgg agaacctgtt aagggtaacg atacgcggtt ggtataataa
     3301 agatgtgcgt gacctggaac caggtaatgg aacaaacgtt gagaagagcg gtcagcaagc
     3361 tggtaccgaa gagtggcagc acaagatggc tcgtgtggac ggaggcagca taaggggacg
     3421 aattgataag aacattgtag catggcttta tgctgatcac ggcgcagaca ggatatataa
     3481 agattcaccg ctgaaaaatg ttaatgccaa aggttggacc gacctatatc gcatgcccaa
     3541 atatatgtac tacctatggc aggcaaacta tcttgaacga cctatgatat ttatccatcc
     3601 tcatcattgg agggctcaat atctggggca aaaaaagaat attcaggttg acagtaactg
     3661 tgatgttgtt gagttgttcg tgaacggcaa aagcattggc aaacaacatc cgtcgaaaga
     3721 aaatttccat accgtagagt acactgatgt agctgtagaa aaaggtatta tcaaagccat
     3781 aggtaccaag gacgggaaga aaaccgaata tcagatttcc atggccggaa atcctgctaa
     3841 aatagtgctt aagtcatccc acgcggctat tccggccgac agatcgggac ttgctataat
     3901 aactgcggat atagtggatg ccaaaggtgt gcatgtatac ggagcgacaa acactctaaa
     3961 atgggaagta atcggcgatg gttcactcgt gggacatcat acatacggaa ccgatataaa
     4021 taaaaattcg gatgtgtctg gatgcggata tatcgacccc cctgtatcta atgttgtaag
     4081 atcgtcaaat aaagccggta cgataaccat aaaagtatct gcagaaggtc ttcaggatgg
     4141 caccattaca ttatctacat acaaatcggc taaaccagca acaggtatta ctgagcctga
     4201 actgagcgat gaagggcgta tggtaactat gcgcgatacg tcattcaacg aagttgttga
     4261 atatgttgtt gagatgaaac gaattgtttc gcctgagcag atcaaggcct cagatagcgt
     4321 tgagtacagg aaagtgatga cacgttttgt cgttgaacgg aatcctggca ttgataaaaa
     4381 gagtattgaa tttgaaaagc taattaaacg gctgaccata tatattgaaa atgtaaacgg
     4441 cgagctgacc gaagacgact ataacttcat cgcacgtacg tttaacgaca ccaggatgct
     4501 ggctaaaatc atcgagacaa agaatttcca ccctgattat gcaaaagcac ttagagaata
     4561 ctattctcat gcgatgcttg ttgagggtaa gatggttgat gtcagcaaag aaagcgagaa
     4621 aattaatcaa ataccaagag agcttgatat tgtggtatat gtaacgccgg aagagactaa
     4681 aatcaacaat gggctggagt atgaaagaac gaagtactac aacagaagca atatctccga
     4741 cctcgatgaa atcatcacta ttcttcatcc tgaatatgca aaaatgaacg atcaggtgaa
     4801 aactaaagtg aagaaatatc tggttcagat caatccgatg cttattggta atgatggtaa
     4861 ataccgtata gctgaacaga gttgtattgc aataccaaaa aaaatgataa accaaataaa
     4921 aactaattga aatgaagcaa aaaattctac tgcgaagaat gataaggact ttactcctta
     4981 tgttctcggt taatttgttc gcaatctgga atgttacggc ccaagatcat aaagtcagtg
     5041 gtatcgtaac cgattccaaa tcaggcgaac cgatcattgg tgcaaccgta tatgttgaag
     5101 gtacttccaa tggaaatatc accggagcgg atggtaagta cgaattaaca atcgacacaa
     5161 acagcaaatt atcgatctcg tttgttggct acattaagca gactatagag gtaaacggac
     5221 gtactcaaat tgatatcgct cttgtagcag acaaccaaga gatggaggaa gttgtagttg
     5281 tcggttatgg cgttgttaaa aaacgtgatc ttacgggtgc aatttcaaac gtaaaaagcg
     5341 accagattgt caaaatggct acttcaggag taaccggagc tcttgtcggt aaagtggccg
     5401 gtcttcagat cactaataca agcggtggcc cgggcgatgg gaacaatgtc gttcgtatcc
     5461 gtgggggtaa ctctataagt ggatcaaacg accctctatg ggttatcgac gggtttatca
     5521 gtgcgggagg agcatcaagc actcctgctg aagacatcga atcggtagag attctgaaag
     5581 atgcgtcagc tacagcaata tatggtgcgc gcggtgctaa tggtgtaatt atcgtgacaa
     5641 caaagcgcgg caaagccgga gcaaccaatg ttaccgttag aagcaatgtc ggtgtcaact
     5701 ggatgagtag ggagttacct cttatgaatg gtccccagac atatgactat tggaacgctt
     5761 tcgataagaa ctatctcgac tccagaattg atcctaaagc aaatttcgac tggataaatg
     5821 cagttacccg caactccttg aaacaaaccc attacatagc tgtaagtggt ggcaaagagg
     5881 gttacgcata taacgtgtct gccgaatatg cagcagatga agggattatc aaatacaact
     5941 cagatactaa acgtcttaat gtaagagcag ctttcgattt tgacctcagc aaaaaggtga
     6001 agatgggtat cactacccgc tatcgctatt cagaggacaa cactgtcgga gatggagact
     6061 tcttccagat gatacttcgt atgtcgcctc ttgtggctcc gttcaaagag gacggatcat
     6121 acaactttaa tcttaatcat ggtaatctaa gtgatgctct gggggttgat ctgtactcca
     6181 aagtcaaggg taatcccatt caatatgtaa gagagagcct aaatccgtca agaggaggat
     6241 tattcaacgg acaagcattc ctgaattacg aaccgatcaa agggttaatc ttccgtacgg
     6301 aattcagtta caacaaaaat aataagtgga atcaaagcta caagccctcc acaatagatg
     6361 taatgacacc tgccaaactt caacactccg aaggcgagtc ttacgagtgg gtaaacacgg
     6421 ctacatacaa aaaagatttc ggccagcaca gcatcaatac agtggtcggg caaactttac
     6481 aaagtgacat taacagtttt ctcagcggaa gtaacaaata tttcgcggct gacggtttta
     6541 cttggaacaa cctgggacaa ggtaacccga ttgaaatcgg agatataggc gtagggtcag
     6601 gatataagag cacatctatc ttgtcgtacc tcgcacgcgt gaactacgtt tataacgaca
     6661 agtatctgtt taccgtaagc ggacgtgcgg acggtgcttc taaattctcg aaaaacaata
     6721 aatggggtta tttcccttcg ggagctgtga gctggagagc cagcgaggaa gattttattg
     6781 aaaaaatgga tctgttctcg acacttaaat tcagagcttc tgtcggtata acaggtagtg
     6841 aagctattaa accatacgaa acgcttggtt tgatgtcggg gggcactatt cttataagct
     6901 caagcatcat tcctccatat ggaacgccta cacagatatt catatccgat aaactcgcaa
     6961 acgataacct gacatgggaa tcaactgttc aaaccgactt cggcatcgat atgggtttct
     7021 tcaaaaacaa actttctctg gcactcgata tttataataa agatactcgc gacctgttgc
     7081 tttatgttcc ggtacttcct gaatcaggtt acgacgttaa actcagtaat gtgggcaagg
     7141 taaacaataa aggtatcgaa ttcagcatca atgcgattcc tgttaccagt aaaaacttcg
     7201 aatggtctac aaacctgaat ctcagccgta ataaaaacaa agtggtatcg cttggaggcg
     7261 cttcaaaaat cgaccagtcg tcaggagata tgcccgaaac aacgtatctg gaagttggcc
     7321 gtccact
//
LOCUS       DAOSDR010000130         7318 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrimonas sp. isolate AMR_MDS_1826
            NODE_3722_length_7318_cov_4.399972, whole genome shotgun sequence.
ACCESSION   DAOSDR010000130 DAOSDR010000000
VERSION     DAOSDR010000130.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863355
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrimonas sp. (wastewater metagenome)
  ORGANISM  Petrimonas sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Dysgonomonadaceae; Petrimonas.
REFERENCE   1  (bases 1 to 7318)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7318)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:10:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,720
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 35
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7318
                     /organism="Petrimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3722_length_7318_cov_4.399972"
                     /isolate="AMR_MDS_1826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2023866"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..471
                     /locus_tag="PLI30_06410"
     CDS             <1..471
                     /locus_tag="PLI30_06410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI79220.1"
                     /translation="ARSIAARLYGENHMETATSTDNLGGIFMKLGATDSAAYYFRTGL
                     NIRHHLYPERNNTHVMISTNNMLSLFVKTQQPDSVYKYLKEALRVGKSTKVQARQRAV
                     TYWQAGEFYEQIARPDSARYYYERALAENRSYLPENDERIKSVLEKLKSVGYKE"
     gene            complement(575..>1490)
                     /locus_tag="PLI30_06415"
     CDS             complement(575..>1490)
                     /locus_tag="PLI30_06415"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOI79221.1"
                     /translation="SIHASFQMLPPDLYTLSLIANPMEGGSVYGAGNYEPGVLITVSA
                     LAYEGYQFINWTLEGQVVSVQTSFAFTMPAEDVNLVANFEEGTPVLCSFSQGYWFSKP
                     GVVWPYNVIVGGLSFTQEEGKQFWPPNTPTKIAFTQYAAVYLSGVTLSLFPDLSDAMA
                     VIDDYFASEYPLPANGIVSNAAGYIGDWIDRNHCNQTTKFSHRITGSDNSEEDAVPDR
                     AISAKAFPNPFSHQVTIEFQVELSGYVTLELFHLTGERKRELYRGYANAFEKYSMTFD
                     GKSLPAGFYFYRITTGGSTHSGRLIRIN"
     assembly_gap    1491..1590
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1591..4750)
                     /locus_tag="PLI30_06420"
     CDS             complement(<1591..4750)
                     /locus_tag="PLI30_06420"
                     /inference="COORDINATES: protein motif:HMM:NF039835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI79222.1"
                     /translation="MKNQLQFRHLFFLVLMSLFMFTGRENISMAQDAAPFQFPAEDRM
                     KEHIVRKAATLEERGHLYYGTWFRSVTPATERKQVPLQGMGGRSTLTVTNTNDSGEGS
                     LRWAIQQANASPEADIIAFDIPGSGPHYIQPLDRLGYIYNPVIIDGTTQPGWAPGAPV
                     IVLDGSLTPDGQEGFRIFGDAHGSEIRGLVIGGFDRPIPPDQPQAGYNGFAIVIETNN
                     NKIQGNFIGLAPDGVTPFPNVRGVQIMNSSNNLVGGTSPEERNVISGHTAIGVAIFGN
                     EVRPGPISSDNVISGNYIGTDATGTAGLGNPINIQISESAVNTLIGGTSVSQRNIISG
                     ADMNGILIVSPGTLNTQVKGNYVGTDVTGTVAIPNEWTGISVYWGPDQTLIGGAEPGA
                     QNIISGNGQAGIELGAGLGPDELPNTNNVIAGNYIGLDVNASPMSNEVGILINGVSQY
                     NTVGGTTAAERNIISGNNSDGIILGGDQVRFNQIFGNFIGTTPDGTAGYAETTGYGIY
                     VGYESSNNVVGGLNPGEGNLISGNYGHGVIVAGSTSIQNNILGNLIGTNASGTGAVGN
                     RAGGIGISNSTSTLIQGNVISGNGNSGSTAGIEVIGNTARATIKSNKIGVDITGQLAI
                     PNIGNGIILRAPSNTIGGNNTGDFNIISGNTSNGILLLSLTGSADQNNIWGNYIGTNP
                     EGLAIGNGANGIDIGGGIRNRIGGIIPARQNKIAYNSGRGISMSDFINLVPVRNEILG
                     NSVYSNSGHGILLGNYSSIPGNDPGDADTGPNQLQNFPEVTSATFGGGQLEITYFVSS
                     DPANSAYSIRVEFFSTNPNERQGKIFLGFDSFGTADFYAGAKTVTLTIPEELGFIAGQ
                     QITATATDNLGNTSHFGTPVTTQEGQTYFTITSSAGTGGSIDPSGETLVEEGGSQLYT
                     ITPEAGYEISELLVNGESTAAVATYLFENVTSDQSIEVSFQLIQSDLFSLTLEVNPIG
                     SGTVAGEGLYEEGQMVEITASPNTGYEFVNWVWEGQEVSDNAVFTFEMPAENLIISAN
                     FEKQTFTITASAGTGGSIDPSGV"
     gene            complement(4928..5626)
                     /locus_tag="PLI30_06425"
     CDS             complement(4928..5626)
                     /locus_tag="PLI30_06425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI79223.1"
                     /translation="MRILSIIFILALLSPALQAQKISRTLENHNSKTADLVLFGTGMD
                     TPLKLGSIDAKGNLSADLSDIQMSAITDEIKDMYFTELRNVFRFGCGGPDDFGKQGNI
                     PALRGGNIALWDGNEWTGSIFLVSDTELKPWLEDEGYNPAIKATFWDIVYAGEDVKLN
                     LNCNNEIGLESGNVEVSYTYSLDLKKGFNWLEYTIEEVYNTNPEETASFPSKVKISNL
                     SNPERMKWMANYFF"
     gene            complement(5908..>7318)
                     /locus_tag="PLI30_06430"
     CDS             complement(5908..>7318)
                     /locus_tag="PLI30_06430"
                     /inference="COORDINATES: protein motif:HMM:NF025253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tail fiber domain-containing protein"
                     /protein_id="HOI79224.1"
                     /translation="FIGTTDNNDVVFKRNNVLAGRLNLDLENTSFGVNALMANISGVN
                     NTATGSFALNSNTYGNYNTAHGTGALANSTIGNDNSATGVAALYFNSSGSNNTAHGRS
                     SLYSNTTGSDNTANGFFALANNTTGNNNTSSGSSALFQNTTGNNNTANGRSALQSNTT
                     GYNNTAIGWESLKSNTTGIYNTAAGVRALLSNTEGYLNTANGYDALRSNTTGNNNTGT
                     GSGSLYANTTGNDNTAIGFEALTYNTTGSRNAANGHSALYSNTTGNNNTANGFEALKS
                     NTSGYNNTAIGFGALFNTTGNNNTGIGFDARVPNAAGSNQVRIGSTTVTYAGVQVAWT
                     ITSDRRWKENVQTSELGLSFINALHPVSYVRKNDENHKTEFGFIAQDLEKTLQEFNVT
                     NSGILTRDDEGMLSLRYNDLIAPMVKAIQELKAENDLLKTDNQALKVELETLTTQGRH
                     NENRLEKLEKLFGVTAENK"
BASE COUNT         1785 a         1689 c         1660 g         2084 t
ORIGIN      
        1 gccaggagta tcgcagcccg gctttatgga gaaaatcata tggaaacggc aacctccacc
       61 gataacctgg gcggaatatt tatgaaactc ggagctaccg acagcgccgc atactatttc
      121 agaaccggcc ttaacatccg ccaccacctg tatccggaac gtaataatac acacgttatg
      181 atatccacca acaatatgct ttcgcttttc gtaaagacac aacagcccga ttcggtctac
      241 aaatacctga aggaagccct tagagtaggc aaatccacga aagtgcaggc aaggcagcgg
      301 gctgtcacct actggcaggc cggtgaattc tacgaacaga ttgcccgtcc agattccgcc
      361 cgttattatt atgaaagagc cctcgctgaa aaccgttcgt atttacccga aaacgatgag
      421 cgtataaaaa gtgttctgga gaaattgaaa agtgtcggat acaaagaata aggacttgat
      481 taacggacaa tagtagtttg ggatttgaat agataatcgg gggcggacgc aaatttaatg
      541 cgtccgcccc cgattattaa tggttgcaaa cagattaatt aatcctgatt aaccgcccgg
      601 agtgcgttga tcctcccgtt gttatgcggt agaagtagaa tccggccggg agagacttcc
      661 cgtcaaaggt catcgaatat ttttcaaaag cattggcgta accacgataa agctcacgtt
      721 tgcgttcacc ggttaaatgg aacagttcga gagtcacata accgcttagt tccacttgaa
      781 attcgatggt aacctgatgg ctgaaaggat tggggaaagc ctttgcacta attgcccgat
      841 ccggtacagc atcttcttcc gaattgtcgg agcccgtgat tcgatgtgag aatttcgtag
      901 tttgattgca atggttcctg tcaatccagt caccgatata gccggctgca ttgctcacta
      961 ttccattggc aggcagtgga tattcgctgg caaagtagtc gtcaatgaca gccatagcat
     1021 ccgacaggtc agggaataag ctgagagtaa ccccgctgag atatacagca gcatattggg
     1081 tgaacgcaat ttttgttggg gtattgggag gccagaattg ttttccttct tcctgggtaa
     1141 agcttaatcc ccctacaata acgttataag gccataccac tccgggtttg gaaaaccagt
     1201 atccttgtga gaacgaacaa agcaccggtg tcccctcctc aaagttcgca accagattca
     1261 cgtcttctgc cggcattgta aatgcaaaag aggtttgcac agaaaccacc tgaccctcaa
     1321 gggtccagtt aataaactgg tatccttcat aagccagagc cgagacggtg atcaggactc
     1381 ccggttcata attgcctgct ccataaacac tgcctccctc catagggttt gcaatcaggc
     1441 tcagggtgta cagatcgggc ggcagcatct ggaagctggc gtgaatgctg nnnnnnnnnn
     1501 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1561 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn caacacctga gggatcaatg gatccgcccg
     1621 ttcccgcgga agcggtgatg gtgaaggttt gcttttcgaa attggccgat attatcaggt
     1681 tttctgccgg catttcaaaa gtaaataccg cattgtctga aacttcctga ccctcccaaa
     1741 cccagtttac gaattcataa cccgtatttg gggaagcggt gatctccacc atctgtccct
     1801 cttcatagag gccctcgcct gcgacagtgc cggatccaat tggatttact tccagagtca
     1861 ggctaaaaag gtcactttgg attaattgaa aacttacctc tatgctttga tcgctggtta
     1921 cattttcgaa aagataagta gcaaccgcag ctgtgctttc tccattcacc agcaattcag
     1981 agatttcata cccggcttcg ggggtaattg tatacaactg gcttcctcct tcttccacca
     2041 gtgtttcacc ggacggatca atacttccgc cggtcccggc cgaagaagtg atggtaaagt
     2101 acgtctggcc ttcctgcgtt gtgaccgggg tgccaaagtg tgaggtgttc cctaagttat
     2161 cggtagccgt tgctgttatc tgctgacctg caatgaaacc caattcttca ggaatggtaa
     2221 gggtcaccgt ttttgctccg gcgtaaaagt cggcagttcc aaatgaatca aatccaagga
     2281 atatttttcc ctgcctttca ttgggattgg tgctgaagaa ttccacccgg attgaatatg
     2341 cggaattggc cggatcggaa gacacaaaat aagtaatctc cagttgccct cctccaaatg
     2401 tggcacttgt tacttccggg aagttctgaa gctggtttgg ccccgtatcg gcatcgccgg
     2461 gatcgttacc gggaatactg ctgtagttcc cgagaagaat tccgtgaccc gaattggaat
     2521 aaacggaatt accaagaatt tcattcctga caggtaccag gttaatgaag tcgctcatac
     2581 ttatcccccg gccgctgttg tacgcgatct tgttttgacg tgccgggata ataccaccga
     2641 tcctgttccg gattcctccg ccaatatcaa taccgttggc tccgttacct atggccaggc
     2701 cttccggatt ggttccgata taatttcccc aaatgttgtt ctggtcagca gatcctgtca
     2761 aggaaagcag taaaatccca tttgaggtat ttccggaaat aatgttaaag tctcctgtat
     2821 tgtttccgcc aatggtattg cttggagctc tgagtattat gccgtttcca atattgggta
     2881 tggccagctg gccggttatg tctactccga ttttgttgct tttgattgta gctcttgcag
     2941 tattgccgat gacttcaata cctgctgtgg aaccagaatt cccgttgccg gaaattacat
     3001 ttccctgtat cagggttgag gtgctgtttg aaattcctat tcctccggca cgatttccaa
     3061 cagcacccgt tcccgatgca ttggtaccga tcagattacc aagaatattg ttttgaatgc
     3121 ttgtagatcc ggccacaatt acaccgtgac cataattacc cgaaatcagg ttcccttcgc
     3181 cgggattcaa gcctccaacg acgttgttac tggattcgta tccgacgtat ataccatatc
     3241 cggttgtttc tgcataacct gctgtgccat caggggtagt accgatgaag tttccaaaaa
     3301 tctgattgaa tcttacctga tcgccaccca gtattattcc atcgctgttg tttcctgaaa
     3361 tgatatttct ttcggctgca gtagtgccgc caacggtatt gtactgagac actccattaa
     3421 tcagaattcc gacttcattt gacatagggc tggcgttcac gtccaaacca atgtagttac
     3481 ctgcgatcac attattggtg ttgggaagtt catccggacc caggcctgcc cccagctcaa
     3541 tgcctgcctg accgtttccg gaaataatat tctgggcgcc gggttcagca cctcctatca
     3601 gggtttggtc cggaccccaa taaacagaaa tgcccgtcca ttcattgggg atggcaacag
     3661 tgcctgtaac atccgtacca acgtaatttc ctttcacctg ggtatttagt gtgccggggg
     3721 agacaattaa aattccattc atatctgcac cactgataat gttgcgctgc gaaaccgaag
     3781 tgcctccgat cagggtatta actgcacttt ctgatatttg aatgttgatg ggatttccaa
     3841 gtcctgcagt tcctgtagca tccgtcccaa tgtaatttcc cgagattaca ttgtcggatg
     3901 agattggccc cggccttacc tcattgccaa aaatggctac tccgattgct gtgtggcctg
     3961 aaatcacatt gcgctcttcg ggtgatgtac ctcctaccag attgttcgag ctgttcatga
     4021 tctgcactcc gcgcacatta gggaaagggg tgacgccatc aggtgccagt ccaatgaagt
     4081 tgccctgaat tttattattg ttggtttcta taacaatggc aaacccatta taacctgcct
     4141 gaggctgatc gggagggata ggccggtcaa atcctccgat caccaggcca cggatctcgc
     4201 tgccatgagc atcgccaaaa atgcgaaaac cttcctgtcc gtcaggggtc agacttccgt
     4261 cgagtacaat caccggcgca ccgggagccc agccgggctg tgtggtgccg tcaatgataa
     4321 cgggattata gatatatccc agccggtcca gcggctgaat atagtgaggc ccgctaccgg
     4381 gaatatcaaa cgcaatgatg tctgcttcag ggcttgcatt ggcctgctga atggcccagc
     4441 gcagcgatcc ttcacccgag tcgttggtgt tggttacggt gagggtagac ctgcctccca
     4501 ttccctggag aggcacctgc ttccgttccg ttgctggtgt cacggaacgg aaccaggtac
     4561 catagtaaag gtgcccccgt tcttcaaggg ttgccgcttt tctgacaata tgttctttca
     4621 tgcggtcttc agccggaaat tggaatggag ccgcatcctg tgccatgctt atgttttccc
     4681 ggcccgtgaa cataaataag ctcatcagga ccagaaaaaa taaatgcctg aattgtaatt
     4741 ggttcttcat gatggttaat gatttggtta aaaaattaat taactgagaa aatgagatca
     4801 ttgcaatgaa tccgatatcc atcaaaatca ttgtcatctg gcagtttcac atagtttctc
     4861 agaggattct gttttctgaa tggcagggct ttttcgagaa attcggtaag tttatcggcc
     4921 atatggttca aaaaaaatag ttcgccatcc atttcattct ttccggatta ctgaggttgc
     4981 taattttcac cttagaaggg aatgaagcgg tctcttccgg attggtatta tagacctcct
     5041 caatcgtata ttctaaccag ttgaaacctt ttttaaggtc aagcgaataa gtataactta
     5101 cttcaacatt accgctttcc aggccgattt cattattgca attcagatta agtttaacat
     5161 cctctcctgc atatacgata tcccagaaag tcgctttaat tgcgggattg tagccctcat
     5221 cttccagcca gggttttagt tcagtatccg agacaaggaa aatggaacct gtccattcat
     5281 tgccgtccca cagcgctata tttcctcccc tgagagcagg aatatttccc tgcttcccaa
     5341 aatcgtcggg gccaccgcag ccaaatctga atacatttct cagctccgtg aagtacatat
     5401 cttttatttc atccgtaatt gcagacatct gaatatcaga aaggtccgcc gaaaggtttc
     5461 cttttgcatc gatgctgcca agttttaaag gagtatccat gccggtacca aaaaggacaa
     5521 ggtcggctgt ttttgagttg tggttttcca gcgtccggct tattttctgc gcctgcaggg
     5581 caggggagag caaggccaat ataaaaatga tcgatagaat tctcatgtta tcagaggttt
     5641 taaagtttta tgaattgata atgacttgaa ttataatctg ttgatgtttt ttactcatcc
     5701 gtacattgaa gtaatgcgga cttttttaaa aaaacccgga cagaacactt actgtccggg
     5761 ccagcattgc agaagacaat ttgtttactg tttataaaag aacccaccaa acttattgtt
     5821 ctgatgcatt atgagaaaat ttttggtttt aaccaggtct aactgtttaa ttgtacaagt
     5881 ttatcttctg aagaacacct ctctgcttta cttgttttca gcggttacac cgaataattt
     5941 ctccagcttt tcgagtctgt tttcgttatg tcttccttgt gtggttaaag tttccagctc
     6001 aacctttaag gcctgattgt cagtcttcag caggtcattt tctgctttga gttcctgtat
     6061 ggcttttacc ataggggcaa tcaggtcgtt gtatcgaaga ctcagcattc cttcatcatc
     6121 tcttgtaagg atgccgctgt tggttacatt gaattcctga agtgtttttt ccagatcctg
     6181 ggcgataaat ccgaactcgg tcttatggtt ttcatcattt ttccttacat aggaaaccgg
     6241 gtgtaaggca ttgataaaac tcagccccaa ctcagaagtc tggacatttt ccttccagcg
     6301 acggtcggaa gtgatggtcc atgctacctg tacaccggca taagtaacgg tggtactgcc
     6361 gatgcgcacc tggttgcttc cggctgcatt tggcacccgg gcatcgaagc cgatgcctgt
     6421 attgttgttt cccgtggtgt tgaagagcgc cccaaaacca atggcggtgt tgttatatcc
     6481 tgaagtgttt gacttaagcg cttcaaatcc gttagctgtg ttgttatttc ctgttgtatt
     6541 ggaataaaga gcagaatggc cattagcggc attgcggctt cctgtcgtgt tatatgtaag
     6601 ggcttcaaaa ccaatggcag tgttgtcatt cccggttgtg ttggcataga gtgaaccact
     6661 tccggtgccg gtattgttgt tgcctgtagt gtttgatcgc agtgcgtcgt atccgtttgc
     6721 ggtattaaga tatccttcag tattgcttaa aagtgctctg actccagcag ctgtattata
     6781 gatgccggtg gtgtttgatt tgagcgactc ccagccaatg gcagtattat tgtagcctgt
     6841 ggtattggat tgcagtgcac ttctcccgtt tgcggtattg ttattgccgg ttgtattttg
     6901 aaacagggca gaacttccgc tggaagtgtt gttattccct gtggtattgt tggctaatgc
     6961 aaagaaacca ttggcagtat tatcagagcc tgtagtatta gagtatagtg aacttctccc
     7021 gtgtgcggta ttgttgcttc cggagctatt aaaataaagt gctgctactc cggtcgcgga
     7081 gttgtcgtta ccaatcgtac tattcgcaag ggcgccggta ccgtgggcag tattgtaatt
     7141 tccgtaggtg tttgaattaa gtgcaaatga tccggtagca gtgttgttga ctccgctgat
     7201 atttgccatt agtgcattaa caccaaaaga agtgttttca aggtctagat tcagtctgcc
     7261 ggcaaggaca ttatttcttt taaaaaccac atcattatta tcagttgtgc cgataaaa
//
LOCUS       DAOSDR010000131         7298 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrimonas sp. isolate AMR_MDS_1826
            NODE_3730_length_7298_cov_4.111418, whole genome shotgun sequence.
ACCESSION   DAOSDR010000131 DAOSDR010000000
VERSION     DAOSDR010000131.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863355
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrimonas sp. (wastewater metagenome)
  ORGANISM  Petrimonas sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Dysgonomonadaceae; Petrimonas.
REFERENCE   1  (bases 1 to 7298)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7298)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:10:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,720
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 35
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7298
                     /organism="Petrimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3730_length_7298_cov_4.111418"
                     /isolate="AMR_MDS_1826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2023866"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..537
                     /locus_tag="PLI30_06435"
     CDS             <1..537
                     /locus_tag="PLI30_06435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007365234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)(+) transhydrogenase (Re/Si-specific)
                     subunit beta"
                     /protein_id="HOI79225.1"
                     /translation="SETPDEQPGKPSAGEILRNARSVIIVPGYGMALAQAQHLVRQLA
                     DKLEANGAEVRYAIHPVAGRMPGHMNVLLAEADVPYEQLYEMDAINGDFKDTDAVIVI
                     GANDVLNPAARDAVDTPIYGMPVLNVDEAPEVFICNFDLSPGYAGVENPLYTKRNGVH
                     LMLGDAKESLSTMIAWLN"
     gene            703..1014
                     /locus_tag="PLI30_06440"
     CDS             703..1014
                     /locus_tag="PLI30_06440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006842800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3467 domain-containing protein"
                     /protein_id="HOI79226.1"
                     /translation="MENFNNDNQIQIELTEEIAQGTYSNLAIISHSSSEFVVDFVRIV
                     PGIPKAKVKSRIILTPEHAKRLLLALKDNIDKFEKMNGTISVNNENPGFLPPIGGMGQ
                     A"
     gene            1066..1314
                     /locus_tag="PLI30_06445"
     CDS             1066..1314
                     /locus_tag="PLI30_06445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007480776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4492 domain-containing protein"
                     /protein_id="HOI79227.1"
                     /translation="MESEVKKEKFNWFTMFWEGFISMSKLGRTLWIIVIVKLIIMFAI
                     LKPIFFPDFLKAQVSDESEKAGYVREQMIERSSFDSEE"
     gene            1353..2900
                     /locus_tag="PLI30_06450"
     CDS             1353..2900
                     /locus_tag="PLI30_06450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008663125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HOI79228.1"
                     /translation="MDLLLNASAVGWARGQFALTAGYHWIFVPLTLGLAVIMSIMETM
                     YVRTGDEKWKKTAKFWQIIFGINFAIGVATGIILEFQFGTNWSNYSLFVGDIFGAPLA
                     IEGIVAFFLEATFISIMFFGWDRVSKKMHLASTWLVTLGATLSAFWILVANAWMQYPI
                     GMEFNPETMRNEMVDFWAVAGSPVAINKFFHTVTSSWGLGAAFVVGVSSWYLIKKRHQ
                     DFALRSIKIATIFGLVSFILIAVSGDGSAYEVTQKQPMKLAAMEGLYEGKEGAGLVAV
                     GLLNPKKEAYNDDVNPYLFKIEIPKLLSLMGYRNINAFVPGIKDVIDGGYTLPDGSTA
                     LSFQEKRERGLLAHKALADFQQAKSEGRDTDAANFETIIKDNYSYFGYGFLEKEEDLI
                     PNVPLTFYMFHFMVMVGGYFILFFAVVWYFHSRKKLENFTALQYIGLWSIVLAYLASQ
                     FGWVVAEVGRQPWTIQDVLPVQAAASGVSAGNVITTFILFAVLFTVLLIAEVTIMLKQ
                     IRKGPDF"
     gene            2945..4099
                     /gene="cydB"
                     /locus_tag="PLI30_06455"
     CDS             2945..4099
                     /gene="cydB"
                     /locus_tag="PLI30_06455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008659667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016682 - oxidoreductase activity, acting
                     on diphenols and related substances as donors, oxygen as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HOI79229.1"
                     /translation="MITYEFLQNYWWFIISLLGGLLVFLLFVQGGQSMINSISKTEDE
                     KKLVINALGRKWEYTFTTLVTFGGAFFASFPLFYATSFGGAYWVWMLLLFSFVVQAVS
                     YEFQSKPGNVFGPKTYRSFLFFNGIIGTFVLGVAVATFFTGSEFTINKVNLVSFGTAG
                     NVMSGWDNPLHGLEALGNLRNWCLGLAVLFLARTMASLFFVNRLDHDVLHKRSRKYTL
                     YNGVPFVVFFLAFLIWTLVSDGYAVNPDTQEVYMEPYKYLNNFIQMPVVLVLFLLGVV
                     SVLWGIFGTVVNKSFNKGIWFAGAGTVVAVTMLLLVAGYNHTAYYPSYTNLQSSLTVQ
                     NSSSSEFTLTAMSIVSLLVPFVFAYIVYAWRALEGKKLKLEDLNKDGHAY"
     gene            4227..5138
                     /locus_tag="PLI30_06460"
     CDS             4227..5138
                     /locus_tag="PLI30_06460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="RsiV family protein"
                     /protein_id="HOI79230.1"
                     /translation="MRRKVFVFSFLVAVLLSSLFSSCNQDKRSLFKADNGIEFDTINI
                     EKKYFLDDNPENPSCNLKLTFVYPERISNFDARAARRVFLRTVIGPSYDSLSVQEAVD
                     RYARNYIANYRNDAEIYKVNKPLHDEHASDTAEGLYVTDEELDYLPEVFYSYYENLSD
                     SIVYNQFGIISFQVRQVNNKGGRMSYETVRNYVLDLSSGELMAEGDIFSAGYDLALRP
                     ILQNSLLEAHGVKSVQELEDLGFFGIDEIVPNKNFLINDKGITYTFNKGEYSAYQLQV
                     PEVFIPYTAVRSLLRENSVVSKLARLK"
     gene            5135..5755
                     /gene="rsmG"
                     /locus_tag="PLI30_06465"
     CDS             5135..5755
                     /gene="rsmG"
                     /locus_tag="PLI30_06465"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008022144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HOI79231.1"
                     /translation="MSVIDTYFPSLSAKQKEQFDALFDLYSDWNSRINVISRKDIDNL
                     YLHHVLHSLAIARFIRFQPGTSVIDVGTGGGFPGVPLAVFFPEVRFLLLDSIGKKVKV
                     AREVSQTIGLENVDFIHSRMEDEKRKFDFVVSRAVMPLPDLEKIVRKNISSEQRNALP
                     NGIICLKGGNLASELSPFKNKAEVFSLSSYFPEPFFETKKLVYLPV"
     gene            5796..5870
                     /locus_tag="PLI30_06470"
     tRNA            5796..5870
                     /locus_tag="PLI30_06470"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5831..5833,aa:Val,seq:gac)
     gene            complement(5943..7010)
                     /gene="rsgA"
                     /locus_tag="PLI30_06475"
     CDS             complement(5943..7010)
                     /gene="rsgA"
                     /locus_tag="PLI30_06475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020046538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HOI79232.1"
                     /translation="MKLDDLGYNDKIEKSRIENNLEDFDIGRVISEHKERYIVKTEKG
                     EFEAEITGNLRFSAKGREDFPAVGDWVALTIYDSGFSVIHGILPRFSIISRQAVSQFG
                     EVQIIATNIDYAFLIQAVDRDFNINRLERYLTICYSSDVSPIIVLSKTDLIDEQRMNG
                     ILNDIKLRIKNVPVIAISNETQNGYEAIKKTIEKGKTYCMLGSSGVGKSTLLNNLSGR
                     TIMRTDTISQSTNKGRHVTSHRELIILENGGILVDNPGMREVGIADTTSGLEITFDKI
                     VSLSQNCKFKDCTHTRETGCSILEAVEKGDIDKNSYENYLKLEREKAHFELTVVEKRK
                     KDKELGKIIKNYKKDMNKNKY"
     gene            complement(7028..7177)
                     /locus_tag="PLI30_06480"
     CDS             complement(7028..7177)
                     /locus_tag="PLI30_06480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI79233.1"
                     /translation="MSTSNLKHLFEVLTARPFLTVLMLFVAAVGSVGHGTGDELWKFF
                     SRFLK"
BASE COUNT         1777 a         1711 c         1747 g         2063 t
ORIGIN      
        1 agcgaaacgc cagatgagca acccggtaag ccgtcggccg gagaaatcct gcggaatgcc
       61 cggtcggtga tcatcgttcc cggatacgga atggccctgg cacaggcaca acacctggtc
      121 cgccagctgg ccgacaagct ggaggcgaac ggtgcggaag tgcgttacgc catccatccc
      181 gtcgccggcc gcatgcccgg gcacatgaac gtgctgctgg cggaggccga cgtgccttac
      241 gagcagcttt acgagatgga tgcgatcaac ggcgacttca aagacaccga tgccgtgatc
      301 gtgatcggcg ccaacgacgt gctcaacccg gccgcccgcg atgcggtgga tacccccatc
      361 tacgggatgc ccgtgctgaa tgtggatgag gctccggagg ttttcatctg caatttcgac
      421 ctgagccccg gttacgccgg cgtggagaac ccgctttaca ccaaaaggaa cggcgttcac
      481 ctgatgctgg gcgacgccaa ggagtcgctc tcaacgatga tcgcgtggtt gaattaaaaa
      541 aacagttgca aaaatgtccc tgaaaacggg acatttttga tttaaaacag gccatttccc
      601 cattttccct tacggatagc tttgtagttt aataaatttt atatttcttt gtttggcttt
      661 atttatcttt tccacactta ttaatgttta atttctcggc aaatggaaaa tttcaacaac
      721 gataatcaaa ttcagataga gctcacggaa gaaattgctc agggaaccta ttctaatctg
      781 gccatcatct cacactcctc ttcggaattt gtggtggatt tcgtacggat tgtcccgggt
      841 attcccaagg caaaagtaaa atcgcgcatt atcctcacgc ccgaacacgc caaaagactt
      901 cttctggcgc tgaaggacaa tattgataag tttgagaaga tgaacgggac catttcggtg
      961 aacaacgaaa atcccggttt tctccctccc ataggaggaa tggggcaggc gtagcctctc
     1021 cccctttttt tgtttacatt tattttaaaa atcacacttt tttttatgga atcagaagta
     1081 aaaaaagaaa aattcaattg gttcaccatg ttttgggagg gctttatcag tatgtcgaaa
     1141 ctcggcagga cactgtggat aatagtgatt gtaaagctca tcatcatgtt tgccatactc
     1201 aagcccattt ttttccccga ttttctcaag gcacaggtga gcgacgaatc ggaaaaagcg
     1261 ggatacgtgc gtgaacaaat gattgaaagg tcgtctttcg attcagaaga ataaaaataa
     1321 aattattaac ccaattaatc ttttaaagat ctatggactt attactgaat gcatcagctg
     1381 ttggttgggc gagagggcag tttgccctta cggccggcta tcattggatt tttgtcccgc
     1441 ttacgctggg attggccgtg atcatgtcta tcatggaaac catgtatgtg cgtaccggag
     1501 acgagaaatg gaaaaaaacc gccaagttct ggcaaatcat tttcggtatc aatttcgcta
     1561 tcggtgtggc aacgggcatt atcctggaat ttcagttcgg tacaaactgg tcgaattaca
     1621 gtttgttcgt gggcgatatt tttggcgcgc cgctagccat tgaaggtatt gtagcgttct
     1681 tcctcgaagc aacgttcatt tccatcatgt ttttcgggtg ggaccgggta agcaaaaaga
     1741 tgcacctggc ttcgacctgg ctggtgaccc tcggggccac tctgtcggcg ttctggatcc
     1801 tggtggcgaa cgcctggatg cagtatccca tcgggatgga gttcaacccc gagaccatga
     1861 ggaacgagat ggtcgacttc tgggcggtgg ccggatctcc cgttgccatc aacaagtttt
     1921 ttcacacggt cacctcgtcg tggggcctcg gcgccgcgtt cgtggtgggg gtaagcagct
     1981 ggtacctgat caaaaagagg caccaggatt ttgcgctcag gagcataaag attgccacca
     2041 tattcggatt ggtgtcgttt atattgattg cggtcagtgg tgacgggtcg gcctatgaag
     2101 tcacgcaaaa gcagccgatg aagctagccg ccatggaagg cttgtacgaa ggaaaggaag
     2161 gggccggatt ggtagctgtc ggtttgctca acccgaaaaa ggaagcatac aacgacgatg
     2221 tgaatcccta cctgttcaag atagaaatcc ccaagctgtt gtctctgatg ggataccgga
     2281 acataaacgc gtttgtaccc ggcatcaagg acgttattga cggcggatac acactgcccg
     2341 acggctccac cgccttgtct ttccaggaaa aaagggagcg cggcctgctg gcccacaagg
     2401 cgctggccga tttccagcaa gcgaagagtg aaggacggga taccgatgcc gccaatttcg
     2461 aaaccatcat caaagacaat tacagctact tcggctacgg gttcctggaa aaggaagaag
     2521 acctgatccc gaatgttccc ctcacgtttt acatgttcca ctttatggtg atggtaggtg
     2581 gatatttcat cctgttcttt gcggtggttt ggtatttcca cagcaggaaa aagctcgaaa
     2641 acttcacagc cctgcaatac atcggtttat ggagtatcgt gctggcctac ctggccagcc
     2701 agttcggatg ggtggttgcc gaagtggggc gccagccgtg gactatacag gacgtgctgc
     2761 ctgttcaggc ggcagcgtcg ggagtatctg ccggaaacgt gataaccact tttatcctct
     2821 tcgcggtgtt gttcaccgtt ctacttattg cggaagttac cattatgctg aaacagatcc
     2881 ggaaagggcc tgatttctaa tctttcacag aataatttat acactttaaa aactcaagca
     2941 aattatgata acttacgaat ttctacagaa ttactggtgg tttataatat cgctgctggg
     3001 aggattgttg gtctttcttc tttttgtgca gggtggacag tcgatgatca attcgatctc
     3061 gaagacggaa gatgaaaaga aactggttat aaatgccctg ggccgcaaat gggagtacac
     3121 ctttaccaca ctggtcacgt tcggtggcgc gttctttgca tcgttcccgt tgttttacgc
     3181 caccagcttc ggcggggcat actgggtatg gatgttgctt ttgttctctt ttgtggtgca
     3241 ggccgtatcc tatgaatttc aatcgaagcc gggtaatgtg ttcgggccga agacgtatcg
     3301 gtcgttcctg ttcttcaacg ggataatcgg tactttcgtg ctgggtgttg ccgttgccac
     3361 gttcttcacc ggatcggagt ttaccatcaa caaggtgaat ctggtgagct ttggaactgc
     3421 cgggaacgtg atgagcgggt gggacaatcc gcttcacggc ctcgaagcgc tggggaacct
     3481 gaggaactgg tgcctggggc tggccgttct ttttctcgca aggacgatgg cgagcctgtt
     3541 ttttgtgaac aggcttgatc acgacgtgtt acacaagaga tcgagaaaat acacgctgta
     3601 caacggtgtg ccgtttgtgg tattcttcct ggcttttctc atctggacgc tggtttccga
     3661 cggctatgct gttaatccgg atacgcagga ggtatatatg gaaccctaca agtacctgaa
     3721 caacttcatc cagatgccgg tggtgcttgt tcttttcctg ttgggtgtcg tgtcggttct
     3781 gtggggaatt ttcggtacgg tggtcaacaa atcgttcaat aagggaatct ggtttgccgg
     3841 ggctggcaca gtggtggcgg taaccatgct gctgcttgtg gccggctaca accatacggc
     3901 atattatccg tcgtatacca acctgcaaag ctcgcttacc gtacaaaatt catcctccag
     3961 cgagttcacg ttgacggcca tgtcaattgt ttcgttgctc gttccttttg tgtttgccta
     4021 catcgtttat gcctggaggg cgctggaagg gaagaagctg aaactcgaag acttgaacaa
     4081 ggacgggcac gcctattaaa aattctgtgt ccggtgtcat ttaagaaggg gaacaatcaa
     4141 aaaaaggttg ttccctttta ttttaaccgt ttcagggcag gtgtgaaaaa taaaatgtgt
     4201 atatttgcat acatattttt taacagatga gacgaaaagt attcgtgttt tcattccttg
     4261 tggcggttct gctctcttca ctcttctcct cctgcaacca ggacaagaga agtttgttca
     4321 aggcagacaa cgggatagag tttgatacga taaacataga aaagaaatac tttctggatg
     4381 ataatccgga aaatccctcg tgtaacctga aactgacctt tgtttatccg gaaagaattt
     4441 cgaatttcga cgcccgggca gccagacgtg tttttctaag gacggtgatc ggccccagct
     4501 acgacagctt gtccgttcag gaagccgttg acaggtacgc ccggaattat atcgcgaact
     4561 accgcaatga tgcggagatc tataaggtca acaaaccctt gcacgacgag catgccagcg
     4621 acactgcaga aggcctgtac gtcaccgatg aagagctcga ttatctcccc gaggtgttct
     4681 actcctatta cgagaacctt tccgattcca tcgtttacaa tcagtttggc attatctcgt
     4741 ttcaggtgag acaggtaaat aacaagggtg gccgcatgtc gtacgaaacc gttagaaact
     4801 acgtgctcga cttgtcgagc ggcgagctga tggccgaagg cgacattttc tccgccggtt
     4861 acgacctggc attgcgtccg atccttcaaa actcgttgct ggaagcacac ggcgtaaagt
     4921 ccgtccagga gctggaagac ctgggctttt tcggtattga cgagatcgtt ccgaacaaga
     4981 attttctcat taacgataaa ggaataacct acacgttcaa taagggagag tactcggctt
     5041 accagcttca ggtacccgag gtttttatcc cgtatacggc cgtccgatcc ctgttgcgcg
     5101 agaattccgt tgtctcaaaa ttagcccggc tgaagtgagt gtcatcgata catatttccc
     5161 ctcgctttcc gcgaagcaga aagagcagtt cgacgccctt tttgatttgt attccgactg
     5221 gaactcccgg ataaacgtca tttcgcgtaa ggatatcgat aatctttacc tccaccatgt
     5281 actgcattcg cttgccattg cccggttcat ccgttttcag cccggtacgt ccgtcatcga
     5341 cgtagggacc ggcggtgggt ttcccggtgt gccgctggcc gtgtttttcc ctgaggtccg
     5401 ctttctgctg ctggacagca tcgggaagaa ggtaaaggta gcccgggagg tttctcaaac
     5461 catcggcctg gagaacgtgg acttcatcca ttcgcgtatg gaagacgaga agcggaaatt
     5521 cgactttgta gtgagtcgcg ccgtcatgcc gttgcccgat ctggagaaga tcgtgcggaa
     5581 aaacatctcc tccgagcaga gaaatgcact ccccaacggg attatctgcc ttaagggcgg
     5641 taatctggcc tccgagctgt ccccgttcaa gaacaaggcg gaggtgttct ccctcagcag
     5701 ctacttcccc gaacctttct tcgagaccaa gaagttggtc tatctgccgg tgtagggttg
     5761 tatatttgaa gaaaatgttg tatttttgca aacccgaatt gttagctcag ctggtttaga
     5821 gcgctgcctt gacagggcag aggtcgttgg ttcgaatcca acacagttca ctacatcacc
     5881 ttacttgtct cctccgggat tcataatgtc ggtttaagcg gaggatattt gcatcgtcaa
     5941 gtttagtact tgttcttatt catgtccttc ttataattct ttattatttt ccccaattct
     6001 ttatcttttt tgcgtttctc caccactgtc aattcaaagt gcgctttctc tctttccaat
     6061 tttaagtaat tttcataaga gtttttatct atatctcctt tttcaacggc ttcaagaatg
     6121 gagcagcccg tctcgcgggt gtgtgtacaa tctttgaact tacaattttg agaaagactg
     6181 acaatcttgt caaatgtgat ttctaatccg cttgttgtgt ctgcaatgcc tacttctctc
     6241 atacctgggt tgtcaaccag tatccctcca ttttcaagaa taatcaattc tctatgactt
     6301 gtaacgtgcc gccctttatt tgtactctgg ctaattgtat ctgtcctcat tatggtcctg
     6361 ccagagagat tattcaataa ggtggacttc ccaactcccg aggaaccaag catacaatat
     6421 gtcttccctt tttcaatcgt cttttttatt gcttcatatc cattttgagt ctcgttactt
     6481 attgcaataa ccggtacatt ttttatacgt agtttaatat cgttcagtat cccattcatt
     6541 ctttgctcat ctatcaggtc agttttactg agcacaatga tggggctaac gtctgatgag
     6601 taacaaatgg tcaggtatct ttcaagtctg tttatattga aatcccgatc aaccgcttga
     6661 attaaaaacg cataatcaat gtttgtcgca attatctgta cctctccgaa ttgactaaca
     6721 gcctgcctcg aaatgattga gaaccgtggt aatattccat ggattacaga aaagcctgaa
     6781 tcatagattg tcaatgcaac ccaatcgcct actgctggaa aatcttcgcg accttttgct
     6841 gaaaatctca agttgccggt aatctcggct tcaaattcgc ctttttcggt tttaactatg
     6901 tatctttctt tatgttccga aattaccctg ccaatatcaa agtcttcaag gttattctca
     6961 atccttgatt tttcaatctt atcattatat cctagatcat ctaatttcat atccgtatca
     7021 atctacgtta tttgaggaac ctgctgaaga acttccacag ctcatcaccc gttccatgcc
     7081 ccacactgcc aacggcagca acaaaaagca tgagtactgt caggaagggt ctggcagtca
     7141 gaacttcaaa taaatgtttc aggttagatg ttgacatgaa aataatgttt taagttcttt
     7201 acaagctcgt tatctacttt acaaccgaac gctttttctc tgccgagttt gtttattcta
     7261 cttcttgcat aatccgtttc caaattgttt gattctgc
//
LOCUS       DAOSDR010000132         7286 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrimonas sp. isolate AMR_MDS_1826
            NODE_3740_length_7286_cov_3.824644, whole genome shotgun sequence.
ACCESSION   DAOSDR010000132 DAOSDR010000000
VERSION     DAOSDR010000132.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863355
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrimonas sp. (wastewater metagenome)
  ORGANISM  Petrimonas sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Dysgonomonadaceae; Petrimonas.
REFERENCE   1  (bases 1 to 7286)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7286)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:10:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,720
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 35
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7286
                     /organism="Petrimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3740_length_7286_cov_3.824644"
                     /isolate="AMR_MDS_1826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2023866"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1939
                     /locus_tag="PLI30_06485"
     CDS             <1..1939
                     /locus_tag="PLI30_06485"
                     /inference="COORDINATES: protein motif:HMM:NF025851.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycoside hydrolase N-terminal domain-containing
                     protein"
                     /protein_id="HOI79234.1"
                     /translation="ETKITQEAFISAEDDVLCVRITSTGKEKLNLALALSRFKDASFS
                     VFPDGRIHMDGQIVDIEAPDAYDDNAGSSGPGGKHMRFAGRLAVKSKGGQINPEGQIL
                     KINETDDILVVFTATTDYNLQKLNFDSSLDPGKRADNILTAVQEKSWDEMKSAHVREH
                     STIFNRVSLDLGPSSNDTIPLDKQLAAFGAGAEDNNLMIKLFQFGRYLLMGSSRNPAI
                     LPANLQGKWNEREWAPWEADYHLNVNLQMNYWPSDVTNLSETNRPLINWFTALAELSR
                     PLAKEMYGSDGWFACLATNPLGRFTPSASTPESQFINGVLDPLAGAWMVMNLWDHYEF
                     TRDHDFLKNELFPLLSGASKFILDVLILDQEGRLHFVPSASPENAYIDQETGRQIRIT
                     ATSTYHLSVIRAVFQATLEAAEILGTTEQICEQIETAQHLLPEFTVDKNGKLMEWRDE
                     MVENEPGHRHLSHLLGLHPFALFTSETSNLYEAARKSLDWRQKNGQGGGGWAAAHAML
                     MYAWLYDGEKAVQSLRSLISSGMETNLLNARSIFQIDGNFGATAGMAEMLIQSHRKDN
                     NGNYIVHLLPALPDAWSSGRVKGLRARGGFFMDISWDNQLLKTAVISSPKGGTCTVQY
                     NEKTLEVTLKPGESRDLKKMM"
     gene            1967..4228
                     /locus_tag="PLI30_06490"
     CDS             1967..4228
                     /locus_tag="PLI30_06490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI79235.1"
                     /translation="MEKSSIPIEIWQKAQYVSILLFLLNTHIFAGTRSGMVTDKVNWK
                     EFLSRHDLIWERMPDDYFAAPFLGNGLLGALLYAPEGEKLRLDIGRADVVEHRNSNAN
                     SIVDNGRLPIGHFTLEMNYPFSNANGRLDLYNAETGLNITVPEKGDMTLKTLVFRDYN
                     AIMVEMEVPLGLQCYWMYHPDASIVFRNPKVGKRYLNPLPEWSFIDGIHVCTQKRDAG
                     GSYTIAWKITKIFGGKTRLFVTIQDSYPGKESVELAVEKIKEISIPEKTDEEIKKHRA
                     WWNDFYTRSFISVPHTRMESVYWIQQYKFASMMREEGPLCDLMGPWYKQTSWPGIWWN
                     LNTQMLYSSLHISNQLKLASTLPDYLIKMNDKLIAAAPEKWQYNSAGVSRCTGPDMDE
                     STLTWPSPEAPERSNLTYLCYYLWEYYRVSMDEDYLKNHFFPLLRRSVNLMLHEVYTD
                     ENGIVHTPYGHSPESTEATDTNYDLSSLKWGCQTLLKINKQLHLKDSLELKWQYVVDH
                     LLPFPVDENGYMCSAQLSAPLMHRHWCHLFQIYPYYLTNWDQPQNREVILRSLKYWGS
                     PAIPNTWTQAVISSMYSSVKDGENALKHLNLALASPNFYPNTFHAEGRNPCSETYGGL
                     CRMLLYMLIQSWGDKIRIFPGVPDSWKDVVFHNLRAEGGFEVSAKKENGKVVWVRVKN
                     NTGELCIIEPEFNGTFKISGGKIKKASESTFVLNIEKGKEMILYTKNTADLKIEPVET
                     EEKQYNFYGLKKK"
     gene            4333..6768
                     /locus_tag="PLI30_06495"
     CDS             4333..6768
                     /locus_tag="PLI30_06495"
                     /inference="COORDINATES: protein motif:HMM:NF039200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5703 domain-containing protein"
                     /protein_id="HOI79236.1"
                     /translation="MKKVKLSRIVLSGLFLIQACTIKQNVPENYEQYNIIWRTQSKNA
                     SESMPLVGGDIGCNVWVENGDILFYLQRSGSMDENGQYLKLGRFRLHLSPDPFEGESF
                     FRQELKLKDGYIEIESEKNGSPDVLKTKLKLWIDVFHPVIYIDVKSNQNTDVTVSYES
                     WRTESMELSGEPQKKERFSAFNLEGYPGKVIRSKDIIEQTTDGITFFHRNPTNKLIPE
                     VLIEQQGLEEYAAEITDDLKNRTFGGILFGENFVFGEVGEGIYQQTPFKSWKLKTKNP
                     QKNHLILIATNIGQTETIEEWKENLSKVKDAATKNPEQTFEKTVDWWHQFWNRSYIFI
                     QSKNPDPKNPVWQMGRNYQLFRYQLGGNVFGEYPAKFNGGNLTFDPVLVNEKVVHDPD
                     WRQWGGAVFTAQNQRLLYWPMLKSGDFDAILPQFELYRKGLPGATARVKKYFGHEGAI
                     YCEYTSVPGIAMGDGWGWQNDYNYRIRGEEIPFGDPRANAATGFGEVVEKGIMANGSI
                     AYHWESQVEHAYMILEYHRFTGNDISAYIPFIEQSLIFFDKHYRLRQKMRNGNELDEN
                     GKLVIYPSTSCESYRGAKNPSDLTAGLKACLEALLILDEKYLDTEKRKYYKEYLNSIP
                     DFDYDEVDGDRIIKPAWSWLKYQNVECPQFYPLFPFNRFYLGKDDMTIFRNTWKHGKF
                     PKNMVQSWHQDGIFFARMGMTEEAADYNIKKLQDSERRFPTFWGPGHDWVPDHNWGGS
                     GMIGLQEMLMQTIEDKIILFPAWPKEWDVDFKLHAPGNTTVKCSFQNGKIVQLIVFPK
                     SRTRNIVVNFDNQ"
     gene            7056..>7286
                     /locus_tag="PLI30_06500"
     CDS             7056..>7286
                     /locus_tag="PLI30_06500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI79237.1"
                     /translation="MRLIFTSLATLLLLWCNAQPITPFPNRYQPQPGQLAVPAKLTIS
                     AAEKELAALIPVFARSAKDFYGITVNEKSRNGF"
BASE COUNT         2321 a         1422 c         1623 g         1920 t
ORIGIN      
        1 agaaacaaaa attacccagg aagcttttat ttctgcagaa gatgacgtac tgtgtgtcag
       61 aatcacctct acaggaaaag aaaaactgaa tttagcgctt gcactgagcc ggtttaaaga
      121 cgcatcgttt tcagttttcc cagatggaag gatacatatg gatgggcaga ttgtcgatat
      181 tgaagcgcct gatgcctatg atgacaatgc gggaagttca ggtccaggcg ggaaacatat
      241 gcgctttgcc ggacgattag ccgtaaagag caagggagga cagataaatc cagaaggtca
      301 gatccttaaa ataaatgaaa ccgatgatat tttagtggtc tttacagcta ccactgacta
      361 taacctacag aaacttaatt ttgattcttc actggatcca ggaaaaaggg cagataatat
      421 tttaacggcg gttcaggaaa aatcgtggga tgaaatgaaa agtgcacatg ttcgtgagca
      481 tagtacaatc ttcaacaggg tgagtctgga tctcggacca tcatcaaatg ataccatccc
      541 tctggataaa caacttgcag catttggtgc aggcgccgaa gacaacaacc tgatgattaa
      601 actgtttcag tttggtcgtt atctattgat gggaagttcc cgaaatcctg ccatacttcc
      661 agccaattta cagggcaagt ggaatgaaag ggaatgggcc ccctgggaag cagattatca
      721 tctcaatgtg aatctgcaga tgaattactg gccgtcggat gtaacgaatc tttcggagac
      781 caaccgcccg cttatcaact ggtttactgc acttgccgaa ctgagcagac cactggctaa
      841 ggagatgtat ggttcagatg gctggtttgc atgtcttgcc accaatccgc ttggccgatt
      901 tacaccctct gcttcaactc cggaatccca atttatcaac ggcgtgcttg atccgctagc
      961 tggtgcgtgg atggtcatga atctttggga tcattacgaa ttcacaaggg atcatgattt
     1021 tttaaaaaat gaattgttcc cattgctgtc aggagccagc aagtttatcc tggatgtact
     1081 gatccttgac caagagggca gattgcattt tgtcccctca gcatcgcccg aaaatgcata
     1141 tatcgatcag gaaacaggaa ggcaaattag aatcacagcc acttccacct atcatctctc
     1201 agtgatcagg gcggtttttc aggccacact tgaagcggcg gaaattcttg ggactacaga
     1261 gcaaatctgc gagcagattg aaacagcgca gcatttactc ccggaattta cggtggataa
     1321 aaacggaaaa cttatggaat ggagagatga aatggttgaa aacgaacctg gacatcgcca
     1381 tctttcacat ttactggggt tacatccatt tgcgctattt acgtcggaaa cgtcaaattt
     1441 atatgaagca gcccgcaaaa gccttgactg gcggcaaaaa aacggacagg gtggtggtgg
     1501 ctgggccgca gcacatgcca tgcttatgta tgcctggttg tatgatgggg aaaaggcagt
     1561 acagtcgctc aggtcgttga tttcttcggg tatggaaacc aacctgttaa atgcaagaag
     1621 tatctttcag atcgacggca acttcggggc aaccgctgga atggcagaga tgctgatcca
     1681 gagccacaga aaagataaca acggcaatta tattgtccat cttttacctg cgctgcctga
     1741 tgcctggtct tcaggaagag ttaaaggcct gcgggcaagg ggcggatttt ttatggatat
     1801 cagctgggat aaccaattgc tgaaaacggc tgtaatctct tctccgaaag gaggaacatg
     1861 cacggttcaa tataacgaaa aaacgttgga agtaacctta aaaccgggag agtcacgtga
     1921 tctgaaaaaa atgatgtgat acaagtaaac cagataaaaa caaaacatgg agaaatcatc
     1981 cattcccatt gagatatggc agaaagcgca atatgtgagt attttactat tcttactaaa
     2041 tactcacatt tttgccggaa caagatccgg gatggttaca gataaggtaa actggaagga
     2101 atttctatct aggcacgatc tgatttggga gaggatgccc gatgattatt ttgctgcacc
     2161 gtttctggga aatggattgt taggtgcttt actttatgcc ccagaaggtg agaaactcag
     2221 gttggacatc ggcagagcgg atgtggtgga acaccggaat tccaatgcca atagtattgt
     2281 ggataacgga cgccttccca tcggccattt cacgttggaa atgaattatc ctttttcgaa
     2341 tgcaaacggt cgccttgatc tgtacaatgc cgaaaccgga ctgaatatta ctgtccccga
     2401 aaaaggtgac atgacactta aaacgcttgt ttttcgcgat tataatgcca ttatggtcga
     2461 aatggaagtt cctctgggac ttcagtgcta ctggatgtat catcccgatg catccattgt
     2521 attccggaat ccaaaggtag gaaaacgcta cctgaatcca ttacctgaat ggtcgtttat
     2581 cgatgggatt catgtttgca ctcagaaaag ggatgccggc ggcagctaca ccattgcctg
     2641 gaagattaca aaaatcttcg gcggaaaaac taggctgttt gtgaccatcc aggacagcta
     2701 tcccggaaaa gaatcggtag aactggcagt ggaaaaaatc aaagagatct caattccaga
     2761 aaaaacggat gaggaaatca aaaaacaccg tgcatggtgg aatgattttt acacccgtag
     2821 ttttatatcg gtaccgcata caagaatgga aagtgtatat tggatccagc aatataaatt
     2881 tgcaagtatg atgcgagaag aaggcccgct ttgtgattta atgggaccgt ggtataaaca
     2941 aacaagctgg cctggcatct ggtggaacct gaatactcaa atgctataca gctcgctgca
     3001 tatttccaat caactgaaac tagcttccac attgcccgat tatctgatta aaatgaacga
     3061 caaactcatt gctgctgctc ctgaaaaatg gcagtacaat tcggccgggg tttctcgttg
     3121 caccgggcct gacatggacg aaagcaccct tacgtggccg tctcccgaag cccccgaacg
     3181 ctccaacttg acttatttgt gctactattt gtgggaatat tatagggtct caatggatga
     3241 agactattta aaaaatcatt tcttcccgct tttacgacgt tccgtaaatt tgatgttaca
     3301 cgaggtatat accgatgaaa acggaattgt ccatacacct tacgggcatt ctcctgaatc
     3361 aactgaagct acagatacaa attacgatct ttcgtcgttg aaatggggtt gccaaacact
     3421 attgaaaata aataaacagt tacacttaaa agactctttg gaattaaagt ggcaatacgt
     3481 ggttgatcat ttattaccgt ttcctgtcga tgaaaacggt tatatgtgtt cggcgcaatt
     3541 atcagcgcct ctaatgcatc gccattggtg tcatttgttc cagatttatc cgtattactt
     3601 gactaattgg gatcagccac aaaacaggga agtcatactt cgatcattaa aatattgggg
     3661 atcgcctgct attcccaata catggacaca agctgtaata agttcaatgt attcgtctgt
     3721 aaaagacggc gaaaacgcat taaaacattt gaaccttgcc ttggcttctc ccaatttcta
     3781 tccaaatacc tttcatgctg aaggcagaaa tccttgttcc gaaacctatg gggggttatg
     3841 ccgaatgttg ctgtatatgc ttattcaaag ctggggcgat aaaatccgta ttttccccgg
     3901 agttcccgat agctggaaag atgttgtttt tcacaacctg agagcagaag gagggtttga
     3961 agtttcagca aaaaaagaaa atggtaaagt agtttgggtc agggtaaaaa ataacacagg
     4021 agaactttgc atcattgagc ccgaatttaa tggtacattc aaaatctccg gaggcaaaat
     4081 aaaaaaagca agtgaaagta cttttgttct gaatattgaa aaagggaaag aaatgatttt
     4141 atacactaaa aatacggctg atctcaaaat tgaacctgtt gaaacagagg aaaagcaata
     4201 taatttttat ggtctgaaaa agaaatgaaa tttcctaact gacttaacaa ctataagaac
     4261 cgttcctctc acaggaaaat gactaaagta ggagaattaa aagccattta aaagaacaat
     4321 taattttgac aaatgaaaaa agttaagctt agccggattg ttttgtctgg attgttttta
     4381 attcaggcat gtaccataaa gcaaaatgtt ccggaaaatt acgaacaata taacattatc
     4441 tggaggacac aaagcaaaaa tgcctcagaa tcgatgccgc tagttggcgg agacattggc
     4501 tgtaatgttt gggtggaaaa cggtgatatt cttttttatc tacaaagaag tggcagcatg
     4561 gacgaaaacg gccaatattt aaaactcgga aggtttcgct tacatctaag cccagatccg
     4621 tttgaaggcg aatctttttt ccgtcaggag ctgaagctaa aagacggata tatcgaaatt
     4681 gaatctgaaa aaaatggctc tccagacgtg ctcaaaacga agctgaaact gtggatcgat
     4741 gtttttcacc cggtaattta tatcgatgta aagtcgaatc aaaatactga tgtcacggtt
     4801 tcgtatgaaa gctggcgtac cgaaagtatg gaattgtcgg gcgaaccgca gaaaaaggaa
     4861 cgtttttccg catttaacct ggaaggttat ccggggaaag taattcggtc gaaagatatc
     4921 attgaacaaa ctaccgatgg gataacattc tttcacagaa atcccacgaa taaacttatc
     4981 cctgaagtgc ttattgaaca acagggtttg gaagaatatg ccgcagagat taccgacgat
     5041 ttgaaaaatc gcacttttgg gggcatattg tttggcgaaa atttcgtatt cggtgaagtc
     5101 ggagaaggca tctatcaaca aactccgttt aaatcatgga aactgaaaac taagaaccct
     5161 caaaaaaatc acttgatttt aattgcaacc aatatcgggc agaccgaaac tattgaagaa
     5221 tggaaagaaa acctttcgaa agtaaaggat gccgccacta aaaatccaga acagacgttt
     5281 gaaaaaacag ttgactggtg gcatcagttc tggaaccgaa gttacatttt tattcagtct
     5341 aaaaatccgg atcccaaaaa tccagtttgg caaatggggc gaaattatca gttgttccgt
     5401 taccagctag gaggaaatgt atttggcgaa tatccggcaa aattcaacgg aggaaaccta
     5461 acatttgatc cggtgctggt aaacgaaaag gtagttcacg atccagactg gcggcaatgg
     5521 ggcggggcag tttttacagc ccagaatcaa cgtttgttat attggccaat gcttaaatcc
     5581 ggggattttg atgccatttt gccccaattt gaactctata gaaaagggct gcccggcgct
     5641 acagcgcggg taaagaaata ttttggacac gaaggagcaa tttattgcga atataccagt
     5701 gttcccggaa ttgctatggg agatggatgg ggttggcaga atgattacaa ctatcgtatc
     5761 agaggagagg aaataccctt tggcgatccg cgtgcaaatg ctgcaactgg ttttggagag
     5821 gtagtagaga aaggaataat ggccaacgga tcaattgctt atcattggga atcgcaagta
     5881 gaacatgctt atatgatttt ggaataccat cgcttcactg ggaacgacat ttctgcttat
     5941 atccctttta tagaacaatc gttaatattc ttcgataaac actaccggtt gcgccagaag
     6001 atgcgtaacg ggaatgaact tgatgaaaat ggcaaacttg tgatttatcc atctacttca
     6061 tgcgaatcat atcggggggc aaaaaatccg tcagatttga cagcaggtct gaaagcttgc
     6121 cttgaagcat tgctgatact ggacgaaaaa tacctcgata ctgaaaaaag aaaatattac
     6181 aaggaatact taaatagtat tcctgatttt gattatgatg aggtagatgg cgatagaata
     6241 ataaaacccg catggtcgtg gttgaaatac cagaatgtgg aatgccctca attttatccc
     6301 cttttcccgt ttaatcgttt ttatcttgga aaagatgata tgactatttt cagaaacaca
     6361 tggaaacatg gaaaatttcc taaaaatatg gtgcaaagct ggcatcaaga cggtattttc
     6421 tttgcccgaa tgggaatgac tgaagaagca gctgattata atatcaaaaa gttacaggac
     6481 agcgaacgcc gttttccaac gttttggggc cctggtcacg actgggttcc agatcataac
     6541 tggggaggaa gcggaatgat cggtcttcaa gaaatgttaa tgcaaaccat cgaagataaa
     6601 atcatattgt ttcctgcctg gcccaaagaa tgggatgttg attttaaatt gcatgcaccc
     6661 ggaaatacta ctgtcaaatg cagttttcaa aacggcaaaa tagtacagtt gatagttttt
     6721 ccaaaatcaa ggacaagaaa tattgttgta aattttgata atcaataaag aactataaaa
     6781 tgatcaccaa tattttattt ggcatattca taatttattc cggggcattt caaaggcatt
     6841 ctcaaatcac ataaacaact gatatacaaa tctataaatt catgttattt ttaaaaccat
     6901 caaataagag ttattgagaa agtgctgacc agcggcagga acgattccac aacggagaaa
     6961 attctcaaat cagccaaaat caaaaaaaac agtagttttg agggattttc aaaatctgag
     7021 ggagccttaa acaggcttat taaaataaca ataccatgcg tctaattttt acaagtttag
     7081 ccaccctgtt actgctttgg tgtaacgcac agccaatcac cccatttccc aaccgctacc
     7141 agccgcagcc aggccaactg gctgttccgg caaagctaac catttcggct gccgaaaaag
     7201 agctggccgc cctgatcccg gtttttgccc ggtcggcaaa agatttttac ggaataaccg
     7261 taaacgaaaa atcccgaaat ggcttt
//
LOCUS       DAOSDR010000133         7261 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrimonas sp. isolate AMR_MDS_1826
            NODE_3755_length_7261_cov_3.363308, whole genome shotgun sequence.
ACCESSION   DAOSDR010000133 DAOSDR010000000
VERSION     DAOSDR010000133.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863355
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrimonas sp. (wastewater metagenome)
  ORGANISM  Petrimonas sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Dysgonomonadaceae; Petrimonas.
REFERENCE   1  (bases 1 to 7261)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7261)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:10:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,720
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 35
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7261
                     /organism="Petrimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3755_length_7261_cov_3.363308"
                     /isolate="AMR_MDS_1826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2023866"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..619)
                     /locus_tag="PLI30_06505"
     CDS             complement(<1..619)
                     /locus_tag="PLI30_06505"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019541168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HOI79238.1"
                     /translation="MNRKDNKYLRSYTSTQGRLMAGARALWRANGMKDEQMGKPIIAV
                     VNSFTQFVPGHAHLHDIGQMVKAEIEKLGCFAAEFNTIAVDDGIAMGHDGMLYSLPSR
                     DLIADSIEYMINAHKVDAMVCISNCDKITPGMLMAAMRLNIPAIFVSGGPMEAGKIGD
                     ESIDLIDAMIESADDAVPDDRISQLEKLACPTCGSCSGMFTANSMN"
     gene            complement(694..1779)
                     /gene="leuB"
                     /locus_tag="PLI30_06510"
     CDS             complement(694..1779)
                     /gene="leuB"
                     /locus_tag="PLI30_06510"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009319318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003862 - 3-isopropylmalate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HOI79239.1"
                     /translation="MKLKIAILPGDGIGPEIMEQALNVTRAVCEKFNHEATFTEAIVG
                     ACAIDAVGDPYPPETHEVCMQADAVLFGAIGHPKFDNDPNAKVRPEQGLLRMRKQLGL
                     YGNIRPVMTFPSLIHKSPLRADLVEGADFVCIRELTGGMYFGRPQGRSEDGDTAYDTC
                     VYTREEIERILHLAYKFAGQRRKKLTVVDKANVIATSRLWRQIAQEIAPQYPDIETDY
                     LFVDNAAMRIIQWPKSFDVLVTENLFGDILTDEASVITGSLGMLPSASIGIHTSLFEP
                     IHGSYPQATGKNIANPAAMILSAALMFEYAFNLQEEGAATRRAVNASLEAGVVTEDIA
                     EGGKAYGTSEVGDWNAEYIKSSILVTL"
     gene            complement(1797..3329)
                     /locus_tag="PLI30_06515"
     CDS             complement(1797..3329)
                     /locus_tag="PLI30_06515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014224258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-isopropylmalate synthase regulatory
                     domain-containing protein"
                     /protein_id="HOI79240.1"
                     /translation="MDTTLRDGEQTSGVSFATEEKVSIVRLLIEELNVDRVEIASARV
                     SEGEFKSVQKIAQWANSHGHINKIEILGFVDGNASLDWITAAGCRVVNLLCKGSEKHC
                     THQLRKTAREHLADIKSVIAEAKKRGMDVNVYLEDWSNGMRDSEAYVFEMMDELQHQP
                     ITRYMLPDTLGILNPSETRMFCEKMIGRYPGLHFDFHAHNDYDLAVANVFEAVKAGVK
                     GVHVTVNGLGERAGNAPVTSIVALIHDQLKLSTNVAEDKLYSVSKIVESYSGVRIPNN
                     KPVIGDNVFTQVAGVHADGDKKKNLYHNDLVPERFGRSREYALGKNSGKANILKNLEA
                     LGIEVDDEAAKKVTARIIELGDKKELVSPDELPYIVSDILKNGIDNQEIQILNYSLNL
                     TDGMRPTATVKISIRGEVYQQSAAGDGQYHAFSKAMYKIYKSLNKPTPELLDYVVVIP
                     PGGKTNAYVQTIITWRFDGKIFKTRGLDVDQTAAAIKATIKMLNMIEKGNIPVIRHME
                     LP"
     gene            complement(3383..3979)
                     /gene="leuD"
                     /locus_tag="PLI30_06520"
     CDS             complement(3383..3979)
                     /gene="leuD"
                     /locus_tag="PLI30_06520"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019541165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HOI79241.1"
                     /translation="MEKFKTITSTYVPLPIENVDTDQIIPARFLKATTREGFGDNLFA
                     DWRYEKSGEPKADFVLNNPAYSGEVLVAGKNFGSGSSREHAAWAIAGYGFKVVVSSFF
                     ADIFRNNALNNGILPVVVSEKFLGELFASVGQDPQATVTVNLEEQFIRNNQTGNREPF
                     DINPYKKECLLKGLDDIDFLLSNKEKIEAYEKNRPYVY"
     gene            complement(4017..5402)
                     /gene="leuC"
                     /locus_tag="PLI30_06525"
     CDS             complement(4017..5402)
                     /gene="leuC"
                     /locus_tag="PLI30_06525"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018108519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HOI79242.1"
                     /translation="MNKTLFDKIWDAHVVTTVKDGPSQLYIDRHLCHEVTSPQAFSGL
                     RERGLKVFRPGQTLLTADHNIPTVEQDKPIRDEVSRYQVDTLAKNAREFGLTYYGLRH
                     SKNGIVHVIGPENGFTQPGMTIVCGDSHTSTHGAFGAIAFGIGTSEVEMVLSSQCILQ
                     TRPKTMRVNFEGKLNDGVSAKDMALYMIWQLTTGGATGYFVEYAGEAVRNLTMEQRMT
                     LCNLSIEMGARGGLIAPDETTYNYIQGREFAPKGERWDEAMAYWKTLKSDEGAAFDKE
                     VTFDASQIKPMITYGTNPGMGMPIDGAIPELSAIDEAGKISFEKSLKYMGFEPGQKLE
                     GKPIDYVFLGSCTNGRIEDFRVFTHYVKGKKKAGNVTAWLVPGSWQVRQQIEAEGLDK
                     ILEEAGFELRQPGCSACLAMNDDKVPAGKYAVSTSNRNFEGRQGPGARTILASPLVAA
                     AAAVTGKITAP"
     gene            complement(5440..6930)
                     /locus_tag="PLI30_06530"
     CDS             complement(5440..6930)
                     /locus_tag="PLI30_06530"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002560884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HOI79243.1"
                     /translation="MMERIYVFDTTLRDGEQVPGCQLNTIEKIQVAKALELLGVDVIE
                     AGFPVSSPGDFNSVVEISKAVTWPVICALTRAVEKDIEVAAESLKYAKRKRIHTGIGT
                     SDMHIKHKFNSNREEIIERAVKAVKYARRFVDDVEFYAEDAGRTDNEYLARVVQAVVN
                     AGATVVNIPDTTGYCFPNAYGAKIKYLVDHVDLKNAILSTHCHQDLGMATANTLSGVL
                     NGARQVEVTVNGIGERAGNTSLEEVVMALKSHKDLGFDTNINTTKIYSTSRLVSTLMN
                     MPVQPNKAIVGRNAFAHSSGIHQDGVLKNVETYEIIDPKDVGIDENSIVLTARSGRAA
                     LKNRLTLLGVDLDNGELDDVYQKFLSLADRKKDIKDDDILALVGKESRMNRIQIDYLQ
                     VTCGVGLRNVASIGLNIAGEHFEASATGNGPVDAAIKAVKKIISRETVIQEFLIQAIN
                     RGSDDVGKVHMQVEHNGMLYYGFSANTDIVSASVEAFIDAVNKFVE"
BASE COUNT         1352 a         1952 c         1988 g         1969 t
ORIGIN      
        1 agttcatgga gttggcggta aacattcccg agcatgagcc gcaggtgggg caagccagct
       61 tctcgagctg cgagatgcgg tcgtccggca cggcatcgtc ggccgattcg atcatcgcat
      121 cgatcaggtc gatggattcg tcgccaattt ttcccgcctc catgggcccg ccggaaacaa
      181 agatggcggg gatgttcagc cgcatggctg ccatgagcat gcccggcgtt atcttgtcgc
      241 aattgctgat gcacaccatc gcatccacct tgtgcgcgtt aatcatatat tctatggagt
      301 cggcaatcag gtcgcgcgac ggtaacgagt agagcatccc gtcgtggccc atggcgatgc
      361 cgtcgtccac ggcgatggtg ttgaattcgg cggcaaagca tcccaacttt tcgatttcgg
      421 ctttcaccat ctgcccgata tcgtgcaggt gtgcgtgccc gggcacaaac tgggtgaatg
      481 aattcaccac ggcgatgatg ggttttccca tctgctcgtc tttcattccg ttagccctcc
      541 acagcgcgcg tgcgccggcc atcaggcggc cctgagtgga ggtgtagctc cggaggtatt
      601 tattgtcttt acggttcatt cctgtatgtt cctattttta ttgttttctt ttatttcaat
      661 ctctatttca atcttattct ctatctctat ttcttataaa gtaactaaaa tggagctttt
      721 aatatactcc gcattccagt cccccacctc acttgtcccg taggcttttc ccccttcggc
      781 aatatcttcg gtgacgacgc ccgcttcgag gctcgcgttg acggcccggc gggtggctgc
      841 tccttcttcc tgcaggttga aagcgtattc aaacatcagt gcggcggaga ggatcatggc
      901 ggcgggattg gcaatgtttt ttccggttgc ctgcgggtag gagccgtgga tcggttcgaa
      961 cagggaggtg tgtatgccta tcgaagcgga ggggagcatc cccagcgatc cggtgatgac
     1021 cgatgcttcg tcggtgagga tatcgccgaa gaggttttcg gttaccagca catcgaagct
     1081 cttgggccac tggatgatgc gcatggctgc attgtccacg aacaggtaat cggtctcgat
     1141 gtcggggtat tgcggggcga tctcctgcgc gatctggcgc cacaggcggg aggtggcaat
     1201 cacgtttgcc ttgtccacca cggtaagctt cttgcggcgt tgcccggcaa acttgtaggc
     1261 caggtgcagg atccgttcga tctcttcgcg cgtgtagaca caggtgtcgt aagccgtgtc
     1321 tccgtcttcg ctccgtcctt gcggccgtcc gaagtacatg cctcccgtca gttcgcggat
     1381 gcagacgaag tcggctccct ccaccaggtc ggcgcgaagg ggtgatttgt ggatcagcga
     1441 ggggaacgtc atgacgggac ggatgtttcc gtacagtccc agttgcttgc gcatccggag
     1501 cagcccctgc tcggggcgta ccttggcgtt ggggtcgttg tcgaatttgg ggtgtccgat
     1561 ggcgccgaac aataccgcgt ctgcctgcat gcacacttcg tgcgtctccg gcggataagg
     1621 gtcgccgacg gcatcgatgg cgcaagcgcc gacaatggct tccgtgaacg ttgcctcatg
     1681 gttaaatttc tcgcatacgg cgcgtgtcac gtttaacgcc tgttccataa tctcggggcc
     1741 gatgccgtca ccgggtaata ttgctatctt caatttcatt tttttctttt tcttttttac
     1801 ggtagttcca tgtgacggat caccggtata tttccttttt ctatcatgtt cagcattttt
     1861 atcgtggcct tgatggctgc agccgtctgg tcgacatcca gtccccgggt tttgaagatc
     1921 ttcccgtcga acctccacgt gatgatggtc tgcacgtaag cattggtttt tccgccgggc
     1981 ggaatgacca ccacgtagtc gagcagttcg ggcgtgggtt tgttgaggct cttgtagatc
     2041 ttgtacatgg ccttcgagaa ggcgtggtac tgcccgtctc ccgcggctga ctgctgatac
     2101 acctcccccc ggatggatat ctttacggtg gctgtcgggc gcatgccgtc ggtgaggttc
     2161 agcgagtagt tcagtatctg tatctcctgg ttgtcgatcc cgttcttcag gatgtcggaa
     2221 acgatgtagg gcagctcatc ggggctgacc agctctttct tgtcgcccag ctcgatgatg
     2281 cgggccgtca cttttttggc ggcttcatcg tctacttcta ttcccagcgc ctccaggttc
     2341 ttcaggatgt tggccttgcc ggagttcttc cccagggcgt attcccgcga acgtccgaac
     2401 cgctcgggca cgaggtcgtt gtggtagagg ttcttcttct tgtcaccgtc ggcatggact
     2461 ccggccacct gtgtgaacac gttgtcaccg ataacgggtt tgttgttggg gatacgtacg
     2521 ccggagtaac tttccacgat cttgctgacg ctgtagagct tatcctcggc aacgttggtg
     2581 ctgagcttca gctggtcgtg gatcagggcg acgatgctgg tgacgggcgc gttaccggcc
     2641 cgttccccca ggccgttcac ggtgacgtgg acgcctttta ctcccgcttt caccgcttcg
     2701 aaaacgttgg ctaccgccag gtcgtaatcg ttgtgcgcgt ggaagtcgaa gtgcaggccg
     2761 gggtacctgc cgatcatctt ctcacagaac atccgggtct cggaggggtt cagtatgccc
     2821 aaggtgtcgg gcagcatgta gcgggtgatg ggctggtgtt gcagctcatc catcatctcg
     2881 aacacgtacg cctctgaatc gcgcatcccg ttggaccagt cttcgaggta gacgttcacg
     2941 tccattcccc gttttttggc ttcggcgatg accgacttta tgtcggcgag gtgctcccgg
     3001 gccgtttttc tgagttgatg ggtgcaatgt ttctccgaac ctttgcagag gaggttgacc
     3061 acccggcatc ccgcggcggt gatccagtcg agcgatgcgt ttccatccac aaatcccagg
     3121 atctctatct tgttgatatg cccgtgactg tttgcccatt gtgcaatctt ctgcaccgac
     3181 ttaaattctc cttccgatac ccgcgcggac gcgatctcta cccggtcgac atttaactct
     3241 tcaatgagca agcggacaat gcttactttt tcttccgttg cgaatgaaac gcccgaggtc
     3301 tgttccccgt cgcgcaacgt ggtgtccatt atttctattt tccgctttac catgtgttga
     3361 tgaactgaaa cgtgtgatac attcaataga cgtaagggcg attcttctcg tacgcttcga
     3421 tcttctcttt gttcgacagg aggaaatcga tgtcgtcgag ccctttcagg aggcattctt
     3481 ttttataggg attgatgtcg aacggctccc tgtttccggt ctggttgttc ctgatgaact
     3541 gctcctcgag gttcaccgta actgttgcct gcgggtcctg gccgaccgat gcgaagagtt
     3601 ctccgaggaa cttctcgctg accaccacgg gcaggatgcc gttgttaagc gcattgttgc
     3661 ggaagatatc ggcaaagaag ctcgatacca ccaccttgaa gccgtatccg gcaattgccc
     3721 aggcggcatg ctcgcggctt gacccgctgc cgaagttttt ccctgccacc aggacttcgc
     3781 cgctgtaggc ggggttgttg aggacgaaat cggctttcgg ctcgcccgat ttttcgtacc
     3841 gccagtcggc aaacaggttg tctccgaacc cttcgcgggt agtggctttc aggaagcggg
     3901 cgggaatgat ctggtcggtg tccacgttct ctatcggaag ggggacgtat gtggatgtga
     3961 ttgttttgaa tttttccata tttgtttgat gtactgatgt gctgatcgaa tagtggttac
     4021 ggtgccgtta tctttcccgt tacggcagca gcggcggcta ccagcgggct tgccaggatg
     4081 gtgcgtgcgc cgggcccttg tctcccctcg aagttacggt tggatgtgga gacggcatat
     4141 tttccggccg gtactttatc gtcgttcatg gccaggcagg cggagcatcc cggctgacgg
     4201 agctcaaagc cagcttcttc caggatcttg tccagccctt cagcttcaat ctgctggcgg
     4261 acctgccaac ttccgggtac cagccatgct gtcacgttgc cggctttctt tttccctttc
     4321 acgtagtggg tgaacacgcg gaagtcttcg atgcgcccgt tggtacaact gcccaggaag
     4381 acgtagtcga tcggtttccc ttccagtttt tgtccgggtt cgaaacccat gtatttcaac
     4441 gatttctcaa acgagatctt tccggcttcg tcgatggccg aaagctcggg aatggctccg
     4501 tcgatgggca tgcccattcc gggattggtt ccgtaggtta tcatcggttt tatttgggag
     4561 gcatcgaacg tgacctcttt atcgaaggca gcgccttcgt cggacttcaa cgttttccag
     4621 taggccatcg cttcgtccca cctttccccc ttgggagcaa actcgcgccc ctggatgtag
     4681 ttgtaggttg tttcgtcggg ggcgatcaat cctccgcggg cacccatctc gatgctcagg
     4741 ttgcaaagcg tcatccgctg ttccatggtc aggttgcgga ccgcttcccc cgcatactcc
     4801 acgaagtagc ccgtggcacc gcctgtggtg agttgccaga tcatgtacaa cgccatgtcc
     4861 tttgcactta cgccgtcgtt gagcttccct tcgaagttca cgcgcatggt tttcgggcgt
     4921 gtttgcagga tacattgcga cgataacacc atctccacct cgctggtccc tatgccgaaa
     4981 gcgattgcgc cgaacgctcc gtgggtggag gtatggctgt cgccgcagac gatggtcatt
     5041 cccggctgag tgaagccgtt ctccgggccg atcacgtgaa cgatcccgtt tttggaatgg
     5101 cgaaggccgt agtaggtgag tccgaactcc cgggcatttt tggccagggt gtccacctgg
     5161 taccgggaaa cctcatcgcg gatgggcttg tcttgttcca cggtagggat gttgtggtcg
     5221 gccgtaagca gggtctgtcc gggacgaaag accttcagcc cccgttcccg cagccccgag
     5281 aaagcttgcg ggctggttac ctcgtggcac aaatgcctgt cgatgtacag ttgcgaaggg
     5341 ccgtctttca ctgtggtaac cacgtgcgcg tcccatatct tatcaaaaag cgtcttattc
     5401 atataatttt gtctgttgta tgttatctgt tagctttgtt cattctacaa acttattcac
     5461 cgcatctata aatgcttcca cggatgccga aacgatatcg gtgtttgccg agaagccgta
     5521 gtagagcatc ccgttgtgtt ccacctgcat gtggaccttg cccacgtcgt cgcttccgcg
     5581 gttgatggcc tggatcagga actcctgaat gaccgtttcg cggctgatga ttttctttac
     5641 tgcttttatc gccgcgtcca ccggaccgtt tccggttgcc gatgcctcaa aatgctcccc
     5701 ggctatgttc aggccgatgc tggccacgtt gcgcagcccc acgccgcagg tgacctgcag
     5761 gtaatcgatc tggatgcggt tcatgcggga ctctttcccg accagggcga gtatatcgtc
     5821 gtccttgatg tcttttttcc tgtcggcgag ggaaaggaat ttctggtaaa cgtcatccag
     5881 ctcgccgttg tcgagatcca ctcccagcag ggtcaaccgg tttttcagtg cggcgcggcc
     5941 gctccgtgct gtcagcacga tggagttctc gtcgatcccc acgtctttcg ggtcgatgat
     6001 ctcgtacgtt tccacgttct tcagcacgcc gtcctggtga atgcccgagg agtgagcaaa
     6061 ggcgtttcgg ccgacgatgg ccttgttggg ctggacgggc atgttcatca atgtggaaac
     6121 caaccggctg gtggaatata tcttggtagt gttgatgttg gtgtcgaacc cgaggtcctt
     6181 gtggctcttc agcgccatca ccacttcctc gagcgaagtg ttccccgcgc gttccccaat
     6241 accgttgacg gttacttcaa cctggcgggc gccgttgagg actcccgaga gggtgttggc
     6301 tgtagccatc cccaaatcct ggtggcaatg cgtcgagagg atcgcatttt tcaggtctac
     6361 atgatccacc aggtatttta tttttgcacc gtaagcgttt ggaaaacagt aaccggtggt
     6421 gtcggggata ttgaccacgg tggctccggc gtttacgact gcctgcacca cacgtgcgag
     6481 gtattcgttg tcggtgcggc ctgcatcttc ggcgtagaac tccacatcat ccacaaaccg
     6541 gcgggcatac ttcacggctt ttacggcacg ctcgatgatc tcttcgcggt tggagttaaa
     6601 cttgtgtttg atgtgcatat ccgatgtgcc gatacctgta tggatccgtt tgcgtttggc
     6661 gtatttcagc gattcggcag ccacttcgat gtctttttcc accgcgcgtg tgagcgcaca
     6721 tataacgggc caggtgacgg ctttggagat ctcaaccaca gagttgaagt cgccggggct
     6781 ggataccgga aatccggctt cgataacatc tacccccagc aattccaggg ctttggccac
     6841 ctggattttt tcgatggtgt ttaactgaca cccgggaacc tgttccccgt cgcgcaacgt
     6901 cgtgtcaaaa acataaattc tttccattat aacctgtatt attcgttttt ttacaagaaa
     6961 aaaacctttc ccacacggtg agaaaggttt tctttatttt attgttcttt atttcaatat
     7021 cgatataccg gtctcaccct ctgaagtcct ccagaagtag aatagaaatg actagaatag
     7081 cgatagaatg agtgttcatt gataaaatga aaaaacttgt tcgttaattg tctgcaaaga
     7141 aaaagcaaaa aaacggaata acaaaatatt ttccagtttt tattcaaaaa aaattacaaa
     7201 ttgaaataaa aagagagtta tcaattacaa aatatattcc ctgtaaaatg aaataaactg
     7261 t
//
LOCUS       DAOSDR010000134         7247 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrimonas sp. isolate AMR_MDS_1826
            NODE_3764_length_7247_cov_3.840517, whole genome shotgun sequence.
ACCESSION   DAOSDR010000134 DAOSDR010000000
VERSION     DAOSDR010000134.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863355
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrimonas sp. (wastewater metagenome)
  ORGANISM  Petrimonas sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Dysgonomonadaceae; Petrimonas.
REFERENCE   1  (bases 1 to 7247)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7247)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:10:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,720
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 35
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7247
                     /organism="Petrimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3764_length_7247_cov_3.840517"
                     /isolate="AMR_MDS_1826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2023866"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..322)
                     /locus_tag="PLI30_06535"
     CDS             complement(<1..322)
                     /locus_tag="PLI30_06535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI79244.1"
                     /translation="MEKKEKTKLSVTGNHCVISYFYVIVLVLLLGSCVANNTKGEESA
                     ELMSKAVPVWAEGREKEMNLTLGFRGTFNADPHSEAILRVAASTVYRLFLNGEFVGSG
                     PARAG"
     gene            complement(346..1983)
                     /locus_tag="PLI30_06540"
     CDS             complement(346..1983)
                     /locus_tag="PLI30_06540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004324139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase"
                     /protein_id="HOI79245.1"
                     /translation="MRPILIVCILTISLFSTYAQEDFYKAYRQEWLQKAEDNKPQLVI
                     TEKKPVRVIDIVRDENSFQHWKIVPHSAIDSFYSTTFKKNQSTILDFKEHITGYFTFS
                     IRPTNITPDAPLRLKFTFGEVPSELAVSFEPYTGSLSRAWLQDETVTVTALPQTITIP
                     RRLSFRYVKIELIASPSYDFYVSDIKCNAGTSVTSVPQELAPSTEEIIKKIDQTGLAT
                     LKECMQTVYEDGPKRDQRLWIGDLYLQAMTNNYSFGQHDLTKRCLYLLAGLSHRNGYL
                     HPCIYETPEPRGDERLFLLEYALLFNATLKDYLLATNDKQTALDLWPVAKKQLDIIYQ
                     YVDKDGLMDFARANREWWIFIDWREGLHKEVPLQGVSIFALQETYKLAQLIGKEDEVK
                     NVPPLIKKMKSTARKKFYDKKTGFFAGTINHQVSYASQLWMILSGVATTKEAQTALLN
                     LSHARDVSYPGTPYLYHYYIQSLINSGLHSLAKQELKKYWGGMLEKGADTFWEAYDPN
                     DDFLSPYNFFPVNSYCHAWSCTPVYFIRKYPEIFQLK"
     gene            complement(1997..3199)
                     /locus_tag="PLI30_06545"
     CDS             complement(1997..3199)
                     /locus_tag="PLI30_06545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019537670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 99-like
                     domain-containing protein"
                     /protein_id="HOI79246.1"
                     /translation="MRTFKFSLNLFLFLVALLISSCTSNNRKNNGQESEYILAAFYWP
                     AYHYEPRAEFLFPDKKGEWEIIYNAVPKEEGHQQPKVPLWGYLDEADPEDMGKKIDAA
                     LDYGVNTFIFDWYWYENKPFLEDAVNNGFLKANNNRMNFYLMWANHDATTYWDVDNPN
                     IDSVIWDGEVNREQFNIVVDRVISRYFKEPSYLKINDEPVFCIYELNTLIHGLGGAEK
                     TKEALDYFTRKTKEAGFSGLHLQAVLWGALPSTLVGVPGDSIESQNKVLEFFGFKSLT
                     NYCWAHLQNPDGDYEVWGDASTGMWDKFNNDFSMTYFPNVTISWDANPRFLFKAGYID
                     NSTPQKFEKYLIKAKKFVDNNSIEPKIITLNAWNEWSEGSYLEPDTVWEYGYLEAVKH
                     VFPGNNNQ"
     gene            complement(3220..5805)
                     /locus_tag="PLI30_06550"
     CDS             complement(3220..5805)
                     /locus_tag="PLI30_06550"
                     /inference="COORDINATES: protein motif:HMM:NF028442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HOI79247.1"
                     /translation="MKIDNLQIIRTLSGFLTLIFASLINIFAQNTNPENTAFQINQHN
                     TKLSDELVDLFISPPANAKPRVMWMWMGTSISKEGITRDLETLHEAGFGGTTMFSLTD
                     VNTVFAYDFENSFQKNIVAWTEPWWELVRFAAEESKRLGMDFGMNNCPGYETSGGKWI
                     PVEYSMQQICYSETAAEGGKPISLYIPKPTVDPRAVMLGPVHNPETGKLEKPVIPERN
                     TYFKDIALLAIPDDNKPVSPDQIIDLTDKMNANNTVDCMLPEGKWTIYRFGHTTMGAL
                     IYPAQWKAAGFECDKMNPDAVSFHLDHIISESKKHLKDLVGNTFSHFHFDSYEAGNAN
                     WTPRMKEEFYARRGYDLTPYLLTFADKIFVDSIKTRQFKQDFNATVRDLHADVYFSII
                     QKKLHEANLIFSSEPYGGPWKEEDVLPKVDRVMTEFWTTNNRYTPYKVKPTIEAARKS
                     GQNLIEAEAFSGQPSMSMWSETPHGLKAIGDEAFCEGINRFVLHRFTQQPYAEKYKPG
                     VSMGQWGTHFDHTQTWWEHGKAMFKYWHRCQAMLLYGKNVDDPNAFKVVTNESNISLK
                     SIQRSHQTIHFFFVANVSREQGNVICSFPYAGNKPELWDPVTTTIRDLPAYTIEDGRI
                     HIPLNFSPSQSFFIVFRTPLSHKSSTLSLKNFPNHKVVKDISRDWSVTFDHRWGGPER
                     PVLFSRLEDWTENTDKRIKYYSGTATYHKLFDCEETLLGKDIFVDLGHVKHIARVRVN
                     GTDLGVVWCDPWMTKIPAEILKRNNNEILIEITNVWANRLIGDEQEPEDMEWGMADFG
                     YGKFRYMKKFPDWFLNNQPRPSKGRYCFTTYNYFTRESPLIPSGLIGPVRLLLIDAST
                     TVPLS"
     gene            complement(5816..>7247)
                     /locus_tag="PLI30_06555"
     CDS             complement(5816..>7247)
                     /locus_tag="PLI30_06555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019537668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HOI79248.1"
                     /translation="GINSTLLHVYIMQPYEDRNPGVNAWFGNEFDRKNTWFSQLDVYI
                     QYLKRVNFMLQQGLNVADVAYFIGEDAPKMTGTTDPPLPVGYQFDYINAEVILRDIEV
                     KDGKLTLPHGTQYKVLVLPKLETMRPELLKKIKQLVQDGAVVHGPAPSRSPSLQNQPE
                     ADREVMRTAAELWAEVDGIKVKERTYGKGRIMDGLTLEEVFRSINQIPDCRLPEDNSI
                     HYGHRTMKGIDIYFLSNQTDKETVIQPEFRVTGKQPELWETNNGNIRPLPAFKAKTES
                     TVVPLKLAPYESVFIVFRNKAGKNVMNNLEENYPTPEIIADLKGPWRVTFDPAFRGPA
                     SPLVFDTLYDWTTSRNDSIKYYSGTATYSITFKAPDNKGNKTIELDLGSLTAMAKVKI
                     NGNYAGGVWSYPYKLDITQGVKPGQNELEIEVVNNWMNRLIGDQLLPDHKRETWSFVN
                     PYNTKSKLQPSGLFGPVTIETVEYHN"
BASE COUNT         1858 a         1565 c         1538 g         2286 t
ORIGIN      
        1 gcccggcacg ggccggtccc gatcccacaa actcaccgtt gaggaagagc ctgtaaacag
       61 tagaagccgc aactcgcagg atggcctctg agtgtgggtc tgcgttgaag gtaccccgga
      121 atcccagggt gaggttcatc tctttttcgc ggccttctgc ccataccggg acagctttgc
      181 tcatcagctc agccgattcc tctcctttag tgttgtttgc gacacagctt cccagcaaca
      241 ggacgagcac gatgacgtaa aaataactaa ttacgcagtg attgcctgtg acggataatt
      301 tcgttttttc ttttttctcc ataaaattat tgttggtgtg tagatttatt ttagctgaaa
      361 tatttccgga tattttctga tgaaatagac cggagtacag ctccaggcat gacaatagct
      421 attcaccgga aaaaaattgt aaggggaaag aaaatcgtca ttaggatcat aggcctccca
      481 aaaggtatca gctcccttct ctagcatccc gccccaatat ttttttaatt cttgttttgc
      541 aagactgtgt aacccgctat ttataagcga ttgaatgtaa tagtgataaa gatagggagt
      601 tcccggataa cttacatcgc gggcatggga gagattcaat agtgcagttt gcgcttcttt
      661 tgtagttgct acaccgctta aaatcatcca taactgcgac gcataagaaa cctgatggtt
      721 gattgtgccg gcaaaaaaac cggttttctt atcgtaaaat tttttacgtg ccgtgctttt
      781 catttttttt atcaacggag gaacattttt tacttcatct tcctttccga tcagttgtgc
      841 cagcttatac gtttcttgta aagcaaaaat ggacactccc tgtaaaggca cttctttgtg
      901 taatccttca cgccagtcaa taaagatcca ccattccctg tttgctctgg caaaatccat
      961 caatccatcc ttgtctacat attgatagat gatgtccaat tgttttttgg ctactggcca
     1021 taaatccaaa gcagtttgtt tgtcgttggt tgccagaagg taatctttaa gggttgcatt
     1081 aaagagaagg gcgtattcca ataggaacag ccgttcatca ccacggggtt ccggtgtttc
     1141 atagatgcag ggatgcaggt agccattccg gtggctcagg cctgctaaca aatacagaca
     1201 acgctttgtc aggtcatgtt gcccgaaaga ataattattg gtcatcgcct gtaaatacag
     1261 gtcgcctatc cataaccgtt ggtctctttt gggcccgtcc tcgtaaaccg tttgcataca
     1321 ttcttttaga gtggctaacc ctgtctgatc gatttttttt attatttctt cagtagaggg
     1381 agccagttct tgtggtacag aggttactga agtaccggca ttacacttta tatccgatac
     1441 ataaaaatca tacgaaggtg acgcgatcag ttcaatttta acatacctga aagagaggcg
     1501 ccggggtatg gttatcgttt gtggcaaggc agtaaccgtg acggtttcat cctgaagcca
     1561 ggctctactt aaactacccg tgtatggctc aaaggatact gccagttccg atggcacttc
     1621 accgaatgtg aattttaacc gcaatggagc atccggggtt atgttggtag ggcgaataga
     1681 aaacgtaaaa taaccggtta tatgttcctt gaaatcgagg atcgtcgatt gatttttttt
     1741 gaatgtggtg gagtaaaatg aatcgatagc tgaatgagga acaattttcc agtgttgaaa
     1801 agaattttca tcgcggacaa tgtcgattac ccggacgggt ttcttttcag taatcactag
     1861 ctggggtttg ttgtcttcag ccttttggag ccattcttgt cggtacgctt tatagaaatc
     1921 ttcctgagca taggtactga acaagctaat agttaaaata caaacgatta aaataggtct
     1981 cattctttct tttaggtcat tggttattat ttccggggaa aacatgtttt acagcttcca
     2041 gatatccgta ttcccaaaca gtgtccggtt ccaggtaact cccttccgac cattcgttcc
     2101 atgcgtttaa ggtaattatt ttgggttcga tggagttgtt gtcgacaaac ttttttgctt
     2161 taatcagata cttctcaaac ttttgaggtg tggagttatc tatgtatcct gctttaaaaa
     2221 gaaaacgagg attggcgtcc cagctgattg ttacatttgg gaaataggtc atggaaaagt
     2281 cgttgttgaa tttatcccac ataccggtgg atgcatctcc ccacacttcg taatctccgt
     2341 caggattttg caggtgagcc caacaatagt ttgttaagct tttgaatccg aaaaattcca
     2401 gtactttgtt ctgactctct atactgtctc cgggtacccc cacaagggtt gagggtaatg
     2461 ctccccataa aacagcttgc aaatgcagtc ctgaaaatcc ggcctctttc gttttccggg
     2521 tgaaataatc caacgcctct ttggtttttt cagcccctcc taaaccatgg atgagtgtat
     2581 ttaactcgta aatgcagaag acaggttcat cgtttatctt taaataggaa ggttctttaa
     2641 aatagcggga aatgacccgg tccaccacaa tgttaaattg ttccctgttc acttctccat
     2701 cccagataac tgaatctata ttgggattat ctacgtccca atatgttgtt gcatcgtgat
     2761 tggcccacat taagtagaag ttcatccgat tgttgttcgc ttttaaaaat ccattgttta
     2821 cggcatcctc caggaaaggt ttattctcgt accagtacca atcgaaaata aaggtattca
     2881 ctccgtaatc gagtgcggca tcaatctttt tgcccatatc ctccggatct gcctcgtcca
     2941 ggtatcccca taaagggact ttcggttgtt gatggccttc ttctttaggt acagcattat
     3001 aaatgatctc ccattcccct tttttatcgg ggaacagaaa ttcagcgcgc ggttcgtaat
     3061 ggtatgccgg ccagtaaaag gctgccagaa tatattctga ttcctgaccg ttgtttttcc
     3121 tgttgttgct tgtgcacgaa gagataagaa gagcaaccaa aaacaggaag agattaagtg
     3181 aaaatttaaa tgttctcatt tccttatact atcattaaat taacttaatg gaacagttgt
     3241 agaagcatca attaaaagta aacggaccgg accaatcagg cctgatggta ttaatgggga
     3301 ttctcttgtg aaataattat aagttgtgaa acaatatctt ccttttgaag gtcttggttg
     3361 gttgttcaaa aaccaatccg gaaacttttt catgtaacga aacttgccgt aaccaaagtc
     3421 agccataccc cattccatat cttcaggttc ctgttcatct ccaattaacc ggtttgccca
     3481 cacattcgta atttcaatta gtatttcgtt attgttcctt tttaatattt ctgcagggat
     3541 ttttgtcatc catggatcac accagactac tcccaaatct gtgccgttaa cacgaacacg
     3601 ggcaatgtgt ttgacatgac ctaaatcgac gaaaatatct tttcccaaaa gcgtttcttc
     3661 acaatcgaat aacttgtggt atgtagctgt accgctataa tatttaatcc tcttatcagt
     3721 attttctgtc caatcttcca atcttgaaaa gaggactggt ctctccggac caccccatct
     3781 atggtcaaag gttaccgacc aatcacgtga gatgtctttc acaaccttat gattcgggaa
     3841 atttttaagg gaaagggttg aagatttatg agacaggggt gtcctgaata caatgaaaaa
     3901 actctgtgat ggtgaaaaat tgaggggtat atgaattctt ccatcttcaa ttgtgtacgc
     3961 tggtaaatca cgaatagtgg ttgtgacagg atcccatagt tctggtttat taccggcata
     4021 aggaaacgaa catatgacat ttccctgttc ccgtgaaaca ttggcaacaa aaaagaagtg
     4081 gattgtttgg tggctccgtt ggattgactt cagtgatata ttgctttcat ttgtgactac
     4141 tttaaaagca ttggggtcat caacgttctt tccgtagagt aacatggcct gacagcggtg
     4201 ccaatattta aacattgctt taccgtgttc ccaccatgtt tgagtatggt caaaatgtgt
     4261 accccattgt cccatagaga caccgggttt gtatttctcc gcatagggtt gctgcgtaaa
     4321 acggtgaaga acgaatcgat tgataccctc acaaaaagcc tcatcgccaa ttgctttgag
     4381 cccgtgaggt gtctctgacc acatactcat ggatggctgg cctgaaaaag cttctgcttc
     4441 tataagattt tgtccgctct tcctggcagc ttcgatagtg ggtttaacct tgtagggagt
     4501 atatcggtta ttcgttgtcc aaaattctgt cataacacga tctaccttcg gcaaaacatc
     4561 ttcttctttc cagggacctc catacggttc actcgagaag atgagattgg cttcgtgtaa
     4621 tttcttctga atgatagaga aatagacatc tgcatgaagg tctctcactg tggcattaaa
     4681 atcttgcttg aattgtctag tcttgatact gtctacaaaa attttgtcag caaaagtcaa
     4741 tagataaggc gtgagatcgt atccccttct ggcataaaat tcttctttca tacggggtgt
     4801 ccagttggcg tttcctgctt catagctgtc gaagtgaaaa tgcgagaagg tattaccaac
     4861 aagatctttt agatgtttct tagattcact gataatgtga tccagatgaa aagaaacagc
     4921 atctggattc attttatcgc attcaaatcc agccgctttc cattgagctg gataaattaa
     4981 tgcccccatg gtagtgtgtc caaatcgata tattgtccat ttcccttccg gtaacatgca
     5041 atcaaccgta ttgttggcat tcattttgtc tgtcagatca atgatttgat ccgggctaac
     5101 aggtttatta tcgtcaggaa tggctaaaag agcaatatct ttgaaatagg tgttacgttc
     5161 cgggataacg ggcttttcca attttccggt ttccgggttg tgtaccggac caagcataac
     5221 cgctctcggg tcaaccgtgg gtttggggat gtacaatgag atgggctttc ctccctctgc
     5281 tgcagtttcc gaataacata tctgctgcat ggaatattca acaggaatcc attttcctcc
     5341 acttgtctca tagcccgggc aattattcat tccaaagtcc atccccagtc tctttgattc
     5401 ttcggccgcg aaccggacaa gttcccacca cggttcggtc catgcaacga tgtttttctg
     5461 aaaggagttt tcgaaatcat atgcgaaaac cgtatttaca tcagttaaac taaacatggt
     5521 tgtcccgcca aagccagcct catgtaatgt ttccaagtcg cgagtgatac cttctttgct
     5581 gatgcttgta cccatccaca tccacatgac tcttggcttt gcattcgcag gtggggaaat
     5641 gaacaaatca accaattcgt ctgatagttt ggtattgtgt tgattgattt ggaatgcagt
     5701 gttctcaggg ttggtatttt gtgcgaagat attaatcagt gatgcgaata tcaatgttaa
     5761 aaaaccagat aatgtcctga ttatctgcaa attatctatt ttcatgtgtg tgatattaat
     5821 tatgatattc aacggtttca attgttaccg gtccaaaaag acctgatggc tgtaacttac
     5881 tttttgtatt gtaaggattg acaaaactcc atgtttctct cttatggtca ggaagtagct
     5941 ggtcacctat cagtcggttc atccagttgt ttaccacttc tatctccagt tcgttttgtc
     6001 ccggtttcac cccttgggtg atatccagct tgtagggata ggaccatact ccccctgcat
     6061 aattaccgtt gattttcact ttggccatgg cggtcaggct acctaagtct aactcaatgg
     6121 tcttattccc tttgttatca ggagctttga atgtaataga ataagtggct gttccggaat
     6181 agtattttat agaatcattc cgggaggtag tccagtcgta tagagtatca aacactagtg
     6241 ggctggcagg ccctctgaaa gcgggatcaa aggtgacacg ccagggtcct ttcagatcag
     6301 caatgatttc cggtgtcgga tagttctctt caagattgtt cattacattt tttcctgctt
     6361 tgttcctaaa tacaataaat acactctcgt atggagctag tttcagaggt acaaccgtag
     6421 attctgtttt ggctttgaat gcaggaagag gcctgatgtt accgttgttc gtttcccaca
     6481 gttctggttg tttaccggta accctgaatt caggctggat cactgtttct ttatcggtct
     6541 ggttgctaag gaaataaatg tctattccct tcattgtcct gtgtccgtag tggatggaat
     6601 tatcctcagg cagtctgcag tcggggatct ggttgatgct gcgaaagacc tcctccaacg
     6661 ttaatccatc catgatccgt ccttttccgt aggttctttc tttcaccttt atcccgtcca
     6721 cttcggccca cagctcggca gccgttctca tcacttcccg gtcagcctcc ggctgattct
     6781 gaaggctggg tgagcgggaa ggggcggggc cgtgaactac ggctccatcc tgtaccagtt
     6841 gtttaatttt tttgagaagc tcgggcctca ttgtttcgag ttttggcaac accagcactt
     6901 tatactgtgt gccgtgcggt aaggtgagtt tgccatcctt tacttctatg tcccttagaa
     6961 ttacctcggc attgatgtaa tcgaactggt atccgacggg cagtggtgga tcggtggtcc
     7021 ctgtcatttt aggagcatct tctccaataa aatacgctac gtcagcaacg ttcagaccct
     7081 gctggagcat gaaatttact cttttcaggt attgtatata cacgtccagt tgagagaacc
     7141 atgtattttt ccggtcgaat tcgtttccga accaggcatt cacacccgga ttcctatctt
     7201 catagggctg catgatatag acgtgaagca gagtgctgtt gatccct
//
LOCUS       DAOSDR010000135         7215 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrimonas sp. isolate AMR_MDS_1826
            NODE_3787_length_7215_cov_3.068156, whole genome shotgun sequence.
ACCESSION   DAOSDR010000135 DAOSDR010000000
VERSION     DAOSDR010000135.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863355
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrimonas sp. (wastewater metagenome)
  ORGANISM  Petrimonas sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Dysgonomonadaceae; Petrimonas.
REFERENCE   1  (bases 1 to 7215)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7215)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:10:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,720
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 35
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7215
                     /organism="Petrimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3787_length_7215_cov_3.068156"
                     /isolate="AMR_MDS_1826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2023866"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(5..571)
                     /locus_tag="PLI30_06560"
     CDS             complement(5..571)
                     /locus_tag="PLI30_06560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI79249.1"
                     /translation="MNKLFHIRSETYYLFEISITSDSLNVNLLPDPYRVAEITKENIR
                     QYDFSLFANKYPRFVERIQNPVNKCYVVLDKEKVCYYTWISLTDFIFPRYVKEVRKLD
                     KDVAFLFDSQCAKEYRGNGFHSYMNIFRLQKIGEDNKTKALGLILAGNKPAFKVQLKS
                     GMKITHTLRTFCCNWLNINRIKFVEYEN"
     gene            complement(601..1791)
                     /locus_tag="PLI30_06565"
     CDS             complement(601..1791)
                     /locus_tag="PLI30_06565"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HOI79250.1"
                     /translation="MTKYAKYSIIVILIILPITDLILKTSEVIGEETNRYSFYIRIVA
                     LIIMLVYLLWNINDFINIFRNPVVGAMGLLLAYILVHFSFTYEQGEGIASIAKISYLF
                     VGFFFFYLLALNDDINETDIRWLYALMALIVFVTILQFIPLRETLRSTGLAGMADNKG
                     YTLVSAFPVLLLFYKKKVFPYLIVLVTIGVLIAGKRGAILCLLVSALIIYIFIKKEKR
                     RLTYSTFLYALLALIGFVLLLSYFNEYISAAFVRIFAITEDGGSGRDNLYRLYWDGFW
                     NSDNINLFFGHGLYSGVRNLNTSAIAHNDWLEILYDYGLLCAFLYIGMFIQLLQYLVS
                     SLSQWNKTYYYILLGVSLMLAMRSIISGTFLMTIETIWLYIPLAFVLGKVDNMMYKKE
                     SLSI"
     gene            complement(1873..2805)
                     /locus_tag="PLI30_06570"
     CDS             complement(1873..2805)
                     /locus_tag="PLI30_06570"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021616.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOI79251.1"
                     /translation="MKITAVIPAYNVEKYIQRSINSVLNQTYKAFEVLVVDDGSTDNT
                     AKLIQAYGSKVTYLYKENGGASSARNLGIIKAQGDWIAFLDSDDEWIETHLENFVQTV
                     ARKKDLMWYGAPVKHVDQETGRMLFDYNGKFRKEFINRPYFNDYLSALPPLGFFSTPT
                     MIIKKEIFHKVQFFDINKKNGQDRNMWLRIGLHYPTIGYTGQTGAIVYKRRNSISRNK
                     KFSTDDFIERYLDYEDMIRSLGEDYLKRATPRLQDWIIRKLKECILMNDYQSIVKLKD
                     TLGHYFPFRYKIIVNTLLRFTFLFKVVPIKNKSK"
     gene            complement(2817..4370)
                     /locus_tag="PLI30_06575"
     CDS             complement(2817..4370)
                     /locus_tag="PLI30_06575"
                     /inference="COORDINATES: protein motif:HMM:NF024832.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HOI79252.1"
                     /translation="MGRQNSGTNYTSDIRVENRSSVTYTQDLFSYKLSKNLRSRTVSG
                     LFWSFIDSFAVQGIGFVVGIILARILDPSDFGLIGMMTIFISISQWFVSSGFGQALIR
                     KQDCTQDDYSTVFLFNIIFGGILYIVLFASASFIAHFFNEPQLTPLIKVLGFSILITA
                     LTIVQRTQLTKKIAFKLQTRISVVASVTAGVVAIIMAYYNWGVWSLIVKTLIENGINS
                     VLLWLYSKWRPSLIFSKRAFNELFGFGYKLMLRGLIYTIFNNIYYAIIGKYFSAAQLG
                     YYTRAEQFSNLPSTKINKIVNRVSYPALSELQEDTVKLKHVYRKILISLVYITSICMI
                     ILAALAEPVVIILVGEKWRPSIEYLQLLSIIGLLYPLCDYNLTILKIVNQPSIILKLE
                     IFKRLFSIPIIVIGVILGIKELLVGIIILTWVEFFVNSYFSGKYIAYSIPELLQDILP
                     NISLAVVVGTGIWIYVQLSVVGNIYYLLLGLFIAMILIIVLSEIFRVKGYLYIKEVLI
                     KELINKKKR"
     gene            complement(4304..5434)
                     /gene="wecB"
                     /locus_tag="PLI30_06580"
     CDS             complement(4304..5434)
                     /gene="wecB"
                     /locus_tag="PLI30_06580"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009292124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HOI79253.1"
                     /translation="MMQISEKQRTIRLTIIAGARPNFMKIAPIIHAIEKFNGNEKRIE
                     YRLIHTGQHYDKNMSETFFEELNIPMPDINLGVGGGTQAEQTAGIMIAFEKELTEHSA
                     DLVIVVGDVTSTMACSIVAKKLNTKVAHIEAGIRSWDLTMPEEINRMITDCLTDYYFT
                     TTELAGQNLRNAGAEDKQIFFVGNVMIDTLLANINRLVKPAIFDTLSLKEKEYIMLTL
                     HRPANVDESDQLKALMNEIVAHVHGLPVIFPIHPRTAKIFSELNIKADNLHIVDPMGY
                     LEFNYLVKYSKAVITDSGGITEETTVMNIPCITLRDNTERPETCTIGTNELIGTNPKA
                     IKPTLDKLFSGNWKKGGIPELWDGKTAERIIQVISELRIEVV"
     gene            complement(5431..6756)
                     /locus_tag="PLI30_06585"
     CDS             complement(5431..6756)
                     /locus_tag="PLI30_06585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019539652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003979 - UDP-glucose 6-dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HOI79254.1"
                     /translation="MDQETKIAVIGLGYVGLPLARLFATKYPVVGFDIHKKRVDGLRE
                     GRDHTLEVEDDVLQSVLVKENPFANHRTPSAGLYCSADPNDIRDANIYIVTVPTPVDK
                     NNRPDLTPLLKASETVGKVIGKGDIVIYESTVYPGATEEDCVPVVERISGLQFNKDFF
                     AGYSPERINPGDKEHTVEKIKKVTSGSTPETGKKVDDLYRSVIAAGTHLAPSIKVAEA
                     AKVIENSQRDINIAFVNELAKIFNRMGIDTHDVLEAAGTKWNFLPFKPGLVGGHCIGV
                     DPYYLAQKAQEYGYHPEIILAGRRMNDTMGEYVASQVVKCMINKGICIKGADILILGI
                     TFKENCPDVRNTKVVNIIHALQGYGANVIVLDPWADPGEVEREYGIISHPSLCSYSHF
                     SIPCFQYDAIVLTVAHKEFLGIEWVQFLKPDGVTYDVKGIMEGVVNGRL"
     gene            complement(6771..>7215)
                     /locus_tag="PLI30_06590"
     CDS             complement(6771..>7215)
                     /locus_tag="PLI30_06590"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005868843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HOI79255.1"
                     /translation="VFGRRQDPDSAYAAVIPLFVKKLINHEPPVINRDGEYSRDFTYI
                     DNVIWMNLLALETENPDTLNQVYNTACGEQTTLNQLVAYLKEYLGESDPIIRHIEPIY
                     GPSRPGDIPHSLANIDKAKRLLGYNPLYNVRDGLKKAVKWYRENL"
BASE COUNT         2248 a         1507 c         1164 g         2296 t
ORIGIN      
        1 cccatcaatt ttcatattca acgaatttta ttctattaat atttagccaa ttgcaacaaa
       61 acgttctcaa agtatgcgtt attttcattc cgctctttaa ttgaacttta aatgccggtt
      121 tgtttcctgc aagtattaat cccaatgctt ttgttttatt atcttcacct atcttttgca
      181 gacggaagat gttcatataa gaatgaaaac catttcccct atattctttg gcgcattggc
      241 tgtcgaacag gaatgcaacg tccttatcca gttttcgtac ttccttcacg tatcttggga
      301 aaataaaatc cgtcagtgaa atccatgtat aatagcaaac tttttccttg tccaatacga
      361 catagcattt gttaacaggg ttttgaatac gttctacaaa tcttggatac ttattcgcaa
      421 ataacgagaa atcgtattgc cggatatttt ccttggttat ttccgcaacc ctgtatggat
      481 ccggcaataa attaacgttc aagctatcag aagtaatact gatttcaaac aaataatacg
      541 tctcacttct aatatgaaat agtttgttca acagttttcg taagatagta gctggtctta
      601 tcatatggag agactttcct ttttatacat catattatct actttcccaa gaacaaatgc
      661 aagcggaata taaagccaaa tggtttcaat agtcattaaa aatgtaccgc taataataga
      721 tctcattgcc agcatcaatg aaactccgag caggatataa taataggttt tattccattg
      781 acttaagctg gaaaccaagt actgtaatag ctgtataaac atgcctatat aaaggaatgc
      841 acaaagaagc ccataatcat acaatatctc caaccaatca ttatgtgcta tagccgaagt
      901 gtttaaattt cttacaccac tgtataatcc gtgtccaaaa aacagattaa tattatctga
      961 attccaaaaa ccatcccaat atagtcgata taaattatct ctacctgacc ctccatcttc
     1021 cgttattgcg aatattctca cgaatgctgc cgatatatat tcattgaaat aagagagtaa
     1081 aagtacaaaa cctattaacg ctaacaaggc gtacaggaat gtactgtatg ttaaccgtct
     1141 cttttctttt tttatgaaaa tatagattat cagtgccgac actaacaaac aaagtattgc
     1201 cccccgcttc ccggctatca aaaccccaat agtgaccaat actattaaat aaggaaaaac
     1261 ctttttttta taaaaaagca ataatactgg aaaagcacta accaacgtat atcctttatt
     1321 gtctgccata cctgccaaac ccgtggatcg taatgtttct cgtaaaggaa taaactgcaa
     1381 aattgttaca aaaacaatca gagccatcaa agcatatagc caacgaatgt ctgtttcgtt
     1441 gatatcatca ttcaaggcaa gaagataaaa gaaaaaaaat ccgacaaaaa ggtaacttat
     1501 tttcgcaatg gaagctatac cttctccctg ttcataagta aatgagaagt ggactaaaat
     1561 atatgccaaa agaagcccca tcgctcctac cacgggattt ctgaaaatat ttataaaatc
     1621 attaatattc cacaataagt aaacgagcat tataatcaaa gcgacaatcc taatatagaa
     1681 agaataccta tttgtctcct ctcctattac ttcactggtt ttaagaatta gatcggtaat
     1741 gggaaggatt attagtatta caataatgct gtatttagcg tacttggtca tggtgaatta
     1801 gtaagaatac taagaggttt aatatgccag ttgtttttgc ataacaattt tattatcaaa
     1861 taaatagtat aactatttag atttattctt tattggaact actttaaaaa gaaacgtgaa
     1921 tcttaataat gtatttacta ttattttata tctaaacgga aaataatgcc ctaatgtatc
     1981 tttcagtttc actattgatt gataatcgtt cattaaaata cattctttta gttttcttat
     2041 aatccaatct tgcaaacgag gtgtagctct ttttaaataa tcttcaccta atgaccttat
     2101 catatcttcg taatctaaat atctttcaat aaaatcatcc gtactaaatt ttttatttct
     2161 tgatatagaa ttacgccttt tataaacaat tgcacctgtt tgtcctgtat agccaattgt
     2221 tggatagtgt aacccaattc taagccacat attacgatct tgcccattct ttttattgat
     2281 atcaaaaaat tgtactttat gaaatatttc ttttttgata atcattgtgg gtgtagaaaa
     2341 aaagcccaat ggtggaagtg ctgaaagata atcattaaaa tagggtctat ttataaattc
     2401 tttcctaaac ttcccattat aatcaaagag cattctccct gtttcttgat caacatgctt
     2461 cacaggcgct ccataccaca ttaaatcctt tttccttgca actgtctgca caaagttttc
     2521 cagatgggtt tcaatccatt catcatctga atctaaaaaa gcaatccaat ctccctgcgc
     2581 tttaattatt cctaaatttc tggcgctact ggcccctcca ttctctttat ataaataggt
     2641 tactttgcta ccgtatgctt gaataagttt cgccgtatta tctgtagatc catcatctac
     2701 aacgagtact tcaaatgcct tgtatgtttg gttcaacaca ctgttgatac tccgttgaat
     2761 atatttttct acattatagg ccggtattac agctgtaatt ttcatcttta gtacaattat
     2821 ctcttctttt tattaataag ttcctttatc aaaacttctt taatatatag atatcctttc
     2881 actctaaaaa tctcagataa tacaattatt aagatcatag caataaataa acccagcaat
     2941 aaataataaa tatttcccac tacagaaagc tgcacataaa tccaaatacc cgttcccacg
     3001 accacagcta aagaaatatt gggtaaaata tcttgcaata actccgggat actatatgca
     3061 atatattttc ctgaaaaata actgttcaca aaaaactcaa cccaggttaa aattattatt
     3121 ccgactagta attctttaat tcctaatata actcctatca caataatagg aattgaaaaa
     3181 agtcttttaa aaatttccag tttgagaatt atggaaggtt gatttactat cttaaggatt
     3241 gttagattat aatcgcatag aggatataat aatcctataa tactcaataa ctgaagatac
     3301 tcgattgaag gtctccattt ctctccaact aatataatta caactggttc agccagtgct
     3361 gccagtataa tcatacatat ggatgtaata tagactaaag atatcagtat tttcctataa
     3421 acatgtttta atttaacagt atcttcttga agttcactta atgcaggata acttacccta
     3481 ttgacaattt tattaatttt agtcgaagga agattggaaa attgttcggc acgcgtataa
     3541 tatcccaatt gagcagccga aaagtattta ccaataattg cataatagat attattaaat
     3601 atcgtatata tcaagcctct taacatcagt ttatatccaa aaccaaataa ttcattaaaa
     3661 gctctttttg agaaaattaa tgatggcctc catttgctat acaaccataa taagacagaa
     3721 tttatcccat tttcaattaa agtcttaacg attaaactcc atacacccca gttataatat
     3781 gccattatta tagccacaac accagcagta acagaagcaa caacggaaat tctcgtttgt
     3841 aatttaaatg ctattttttt cgtgagttgt gttctttgga caatagttaa tgctgtaata
     3901 agaatgctaa atcctagtac tttaattaaa ggagttaatt gaggttcatt aaaaaaatga
     3961 gcaataaaag aagcagaagc aaacaataca atatacaaga ttccaccgaa tattatatta
     4021 aaaaggaata ccgtcgaata gtcgtcctgc gtacaatcct gttttcgaat taaagcttgt
     4081 ccaaatccgc tacttacaaa ccattgtgag atagaaatga atatagtcat cattcctatt
     4141 aatccaaaat cgctcggatc cagtattctt gccagaatga tgcctacaac aaatcctatt
     4201 ccctgaactg cgaaactgtc tataaaactc cagaaaagac ctgaaacagt tctactcctt
     4261 aaattcttac tcaatttata actaaataaa tcttgtgtat aagttacact acttctattc
     4321 tcaactctga tatcacttgt ataattcgtt ccgctgtttt gccgtcccac agttcgggaa
     4381 tacctccttt tttccaattg ccggaaaaaa gtttatctaa cgtaggtttt atggcctttg
     4441 gattagtgcc gatcaattca ttggtaccga tagtacaagt ctctggccgc tcagtattat
     4501 cccgtagagt aatacaagga atattcatca cagtagtctc ttctgttatt ccacctgaat
     4561 ctgtaattac cgctttcgaa tatttaacca aatagttaaa ttccagatat cccatcggat
     4621 caacaatatg aaggttatcg gctttgatat tcaattcgga aaatattttt gccgtcctcg
     4681 gatgaatagg aaaaataacg ggcaggccat gcacatgggc aacgatctca ttcatcaatg
     4741 cctttagttg atccgattca tctacattcg caggtcggtg cagtgtgagc atgatatatt
     4801 ccttttcttt cagcgaaaga gtatcgaata tagccggttt cactaatcgg tttatattgg
     4861 caagcaaggt atctatcatc acattgccta caaaaaatat ctgtttgtct tccgctcctg
     4921 catttctcag attctgacca gcaagctcgg tcgtggtaaa ataataatcg gttaagcagt
     4981 cggtaatcat ccggttgatc tcttccggca tggtcaaatc ccacgaacga atgcccgctt
     5041 cgatatgggc gacctttgta tttaatttct ttgctacaat ggaacaagcc atggtggagg
     5101 tcacatcacc caccacgatt accagatcgg cggaatgttc agtcaactct ttctcgaatg
     5161 cgatcatgat gccggccgtt tgttccgctt gggttcctcc tcccactccg aggtttatat
     5221 cgggcatagg aatattcagt tcctcaaaga aagtctcgct catgttcttg tcgtagtgct
     5281 gccccgtatg gataagacgg tattctattc gtttctcgtt tccattaaac ttttcaatgg
     5341 catgaatgat aggagcgatt ttcataaagt taggccttgc ccccgctata atggttagcc
     5401 taatagttct ttgtttctct gaaatttgca tcataaccta ccgttcacaa ctccttccat
     5461 tattcctttc acatcatatg tcacaccatc cggtttcagg aattgcaccc actctatacc
     5521 caaaaactct ttgtgagcca cggtcaggac aatagcgtcg tattggaaac aagggatgga
     5581 aaaatgggaa taggagcata gggatggatg actaatgatg ccatattctc gctccacttc
     5641 tcccggatct gcccagggat caagaacaat aacgttcgct ccgtatccct gcaaggcatg
     5701 tataatattt actaccttgg tattccgtac atccggacag ttttccttaa aagtaatccc
     5761 caatatcagg atatcggccc ctttgataca aatcccttta tttatcatgc actttaccac
     5821 ttgcgaagcc acatactctc ccatcgtatc attcattcgg cgaccggcaa ggatgatttc
     5881 aggatgatag ccgtactcct gtgccttttg cgccaggtaa tagggatcca caccaataca
     5941 atgtccaccc accaatccag gtttaaacgg aaggaagttc cacttggtgc cggcggcttc
     6001 caacacgtcg tgcgtatcaa tacccattcg gttgaaaatc tttgccaatt cattgacaaa
     6061 ggcaatattg atatcgcgct gcgagttctc gattaccttg gcggcttcgg ctaccttgat
     6121 cgagggagcc aaatgggttc cggcagcaat gacagatctg tacaaatcat caactttctt
     6181 cccagtttca ggtgtggaac cagaagttac ttttttaatt ttttcgaccg tgtgttcctt
     6241 gtcaccggga ttgatacgtt ccggagagta accggcaaag aaatctttgt tgaattgtaa
     6301 tccggatatc cgttccacta cagggacaca gtcttcttcc gtagcaccgg gataaacggt
     6361 ggattcataa ataacgatat cgcctttccc aatgaccttc cccaccgttt cactcgcttt
     6421 caacaacggg gtaagatcgg gacgattatt cttgtctacc ggcgtaggca ccgtaactat
     6481 gtatatattg gcatctctga tatcgttggg gtcagcagag caatagagac cagcagatgg
     6541 cgtccggtgg ttggcaaagg ggttttcttt taccaaaaca gattgaagca cgtcatcttc
     6601 cacttccaat gtatggtcgc gcccttctct cagtccatcc acgcgttttt tgtgaatatc
     6661 aaatcccaca acgggatact tagtggcaaa caatctggcc aaaggcaatc ctacgtaccc
     6721 aagaccaatc actgcaattt ttgtttcctg atccattaat atgtgaaatt ttataagttt
     6781 tcccgatacc attttacggc tttttttagt ccgtcgcgca cgttgtatag cggattataa
     6841 cctaaaagcc gttttgcttt atcgatattc gccaaagagt gagggatatc tccaggccgg
     6901 ctgggaccgt aaataggttc aatatgccgg attataggat cggactcacc aagatattct
     6961 ttcagataag ccactaattg attaagtgtg gtttgctcac cacaggctgt attatatacc
     7021 tgattcaggg tatcgggatt ttccgtctcc aaggcaagga gattcatcca tatcacatta
     7081 tcgatatagg tgaaatcacg gctatattcg ccgtcacgat tgataacagg aggttcatga
     7141 tttatcagtt ttttcacgaa cagggggatc accgccgcat atgcgctgtc gggatcctgc
     7201 cggcggccga acaca
//
LOCUS       DAOSDR010000136         7209 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrimonas sp. isolate AMR_MDS_1826
            NODE_3792_length_7209_cov_4.663265, whole genome shotgun sequence.
ACCESSION   DAOSDR010000136 DAOSDR010000000
VERSION     DAOSDR010000136.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863355
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrimonas sp. (wastewater metagenome)
  ORGANISM  Petrimonas sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Dysgonomonadaceae; Petrimonas.
REFERENCE   1  (bases 1 to 7209)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7209)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:10:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,720
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 35
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7209
                     /organism="Petrimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3792_length_7209_cov_4.663265"
                     /isolate="AMR_MDS_1826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2023866"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..421)
                     /locus_tag="PLI30_06595"
     CDS             complement(<1..421)
                     /locus_tag="PLI30_06595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019538190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI79256.1"
                     /translation="MKKFLLSIMMMATISAPAVLTSCGSDDPKPEPPVTESELKGSIT
                     TTQTLDAAITYTITGPVIVEDGGVLNIPAGTMIKAKNGFSNYILVLQGGKINARGTAE
                     KPITLTADIENAKQGHWGGLIINGKAPLSGGTKGSTEI"
     gene            complement(470..3181)
                     /locus_tag="PLI30_06600"
     CDS             complement(470..3181)
                     /locus_tag="PLI30_06600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005679888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOI79257.1"
                     /translation="MISKAKVLFRLSVLLLFFVSASAIYAQTGTIKGTIVDAKTKEPL
                     IGASVLVEETTNGAAADLDGNFVINNVSAGTHTLVASYVAYQPVTKTGIAVETNRETV
                     VDFEMNSDDIILEAVEVVARANRESENILLLEQKKALLATQAVGARELSRKGIGNAEA
                     AVSKISGISKQEGVKNVFVRGLGDRYNATTLNGFLVPSEDPEYKNIALDFFGTDIIQN
                     VGVNKVFSGSSTGDAGGAIIDISSKELINDWAFGAEISGGLNRGTIGVDFLRQDGSNY
                     LGFANNKKPNENKVYFANSLDPTVIKTPVNHSFGISGGKQFKVGADNHPLSFFVVGSH
                     GTNYSYTRELIRNTTSDGTIYQDQQGDKYSQNINQLVLGNINFGINKAHNLAYNFMMV
                     HANNQYVGQYSGKHTESHQDSNDYTGRAIRQQTNDNLLFTHQLISSWKLSNKMKLNAG
                     ASYNRIKGLEPDRRENYLSKQDDGTYILTGSNRQKRFFSELKENDINTKVNLQYKLND
                     EFDNTNSALEIGYNGRFVDDKFEAVEYNFDALSGTFPIDNLQLDDLYNQANYESGKYT
                     MSVGELNTYNVKKNVHSAYAEATYQLTSPLTANVGLRVDNVDMTVNHRVQHVEPGRES
                     IKKTYFLPSLNLKYDLNDKNAIRMGLSKTYTLPQSKEISPYQYVNIGFASQGNPNLKP
                     SDNYNADLKWDYYLSPSELFTVTGFFKHIVNPIGRVDQGNSAGLLSYSNISAFAIVAG
                     IEVELRKNLINRVNTATSKVNRVSLGLNASYIYTDLEVDIVNTPRRKAQLEGAAPFIG
                     NFDVSYSYSNRDKNLLASVVFNYFSNRVHTIGTRNYGNIIEEGVPTIDFVSSYKFNKN
                     FTAKFKASNLLDPSLKLSRRMNSSGENIILNKYKKGINLSLGVSYDL"
     gene            complement(3351..5240)
                     /locus_tag="PLI30_06605"
     CDS             complement(3351..5240)
                     /locus_tag="PLI30_06605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009599020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylase"
                     /protein_id="HOI79258.1"
                     /translation="MKRKLLIRDLTLRDGQQSAFATRMNQSQVDRVLPYYRDANFYAM
                     EVWGGAVPDSVMRYLGENPWDRLKKVSDGVQGASKLTALSRGRNLYGYVPYPDEIIDG
                     FFKNAVSSGLNIMRIFDALNDVDNIKSSIKYIKKYGGLADCAVCYTIDPKYDDEVKIV
                     EKKGFLGLFNKKEEIRIKKENVFTDRYFLQKAKEMLALGADMITIKDMSGLIPPTRVA
                     NLISLFKRELNVPIDFHTHCTPGYGLGSVLSAIVNGADIVDTNIWYFSGGPAAPAIEL
                     VYIFCQKLGIELDVNMEAVAKINAELFGIRRELEAFDTVKQFPNPFNPLTDTLPAAID
                     KLFDEAIAAVQINNETALLEACHQIEAYFNFPKPDERVKNAEIPGGMYTNMVAQLKQF
                     NSLDILEDAMKLIPSVRLDAGLPPLVTPTSQIVGVQAVSSALNLKNGRDKYANASNQF
                     VALVKGEYGKTPVPVDPEFRLKIAGTRDETPYDTSTYKRQENPVLPEFENVRLAKDEK
                     EELLLELFPAVATQYLRKIRETEFASHQSQKTTVTGAGTVPTDAEVKEPEGYITESPM
                     PGSVMKFLVKEGDEVQRGKNLFVLEAMKMENDVISEYSGKVYKIYVKPGDIVQAGEPI
                     MKIVQ"
     gene            complement(5512..7200)
                     /locus_tag="PLI30_06610"
     CDS             complement(5512..7200)
                     /locus_tag="PLI30_06610"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006841441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HOI79259.1"
                     /translation="MWRAIQEMGFEQPMPVQEEVIPQLLANTRDIIALAQTGTGKTAA
                     FGLPIIQKTAIELHAPQTLILCPTRELCLQIAGDLTDFSKYIDDIKILPVYGGSSIES
                     QIKTLKRGVHVIVATPGRLIDLINRKTVSLANVHTVVLDEADEMLHMGFTESIDEILS
                     HVPDNRSMLLFSATMPQEIAKITRKYMSNPMEITIGRKNEGALNVKHTYFMVHAKDKY
                     LALKRIVDYYPNIYGIVFCRTRKETQEIADKLIQDGYDADSLHGDLSQAQRDYVMNKF
                     RLRNLQLLIATDVAARGLDVDDITHVINFGLPDDYEVYTHRSGRTGRVGKTGTSISII
                     HTKEKGKVAQIEKLINKKFEYRQVPKGDIICEKQLFNFVDKIEKVKVNEEEIARLLPS
                     IFKKLDWLEKEDVIKRMVSLEFNRLIDYYQAANEIEEPKEHNSKREERGKGKKFDRNE
                     GGPKKAENGYARFFINIGKMDGANPANLMGFINDYVPGKIRVGRIDLMKNFSFFEVPE
                     ENVKQILKSFKGVFVEDRKLVVELADNNMPENKKYKGDKPKQDFNVSKKGKKRF"
BASE COUNT         1838 a         1587 c         1434 g         2350 t
ORIGIN      
        1 taatttcggt agaccctttt gtcccacccg aaagtggtgc tttaccgtta ataataagcc
       61 ctccccaatg tccttgttta gcattttcaa tatcggcagt cagggtaatg ggtttttcgg
      121 cagtacccct cgcattgatt tttccacctt gcaatacgag aatataattg ctgaatccat
      181 ttttagcctt gatcattgtt cctgccggaa tgtttaatac acccccgtct tcaacaataa
      241 caggaccggt aatggtataa gtaattgctg catccagggt ttgggtagtg gttatagaac
      301 ctttcagttc cgactcggtt accggaggtt cgggtttcgg atcatcggag ccacaagaag
      361 tcagaactgc aggagctgaa attgtggcca tcatcataat actcaaaaga aactttttca
      421 ttttaataca gtttaaaaat ttagttgatt agttgttcgt attcgtcagt tataaatcat
      481 agcttactcc taagctgagg ttaataccct ttttgtattt attcaggata atgttttcac
      541 cgcttgaatt cattcttctg gataatttaa gcgaaggatc cagcaagttt gatgctttaa
      601 atttcgcggt aaagttcttg ttaaatttgt aggaagacac gaaatctatc gtgggaactc
      661 cttcttctat gatattgccg tagttgcgtg taccgattgt atgtaccctg ttactgaaat
      721 aattaaaaac gaccgaagca agtaaatttt tatcgcgatt tgaataacta taagaaacat
      781 cgaaatttcc tataaacggt gcagctcctt ccagctgtgc ttttcttctc ggtgtgttaa
      841 cgatatcaac ttcgagatcc gtataaatat acgaagcatt caatcccagg gatacccggt
      901 ttacttttga agttgcagta ttcactctgt tgatcaagtt ttttctcaac tccacctcta
      961 taccggcgac aattgcaaac gcgctgatgt ttgaatagga gagtaatccg gctgaattac
     1021 cctgatccac tcgtccgata gggttgacaa tgtgtttaaa aaatccggta acggtaaaca
     1081 attccgaagg gctcaaataa taatcccatt taagatcagc attgtaatta tcagacggtt
     1141 tcaggttagg attaccttga ctggcaaagc cgatattcac atattgatag ggtgaaatct
     1201 ctttcgattg cggcaatgtg tatgtcttac tcaagcccat ccgtattgcg tttttatcgt
     1261 tcaaatcata tttcagattc aaactgggca aaaaataggt ttttttgata gattcccttc
     1321 caggctccac gtgctgaaca cgatggttaa cggtcatatc cacattgtct acacgtaaac
     1381 ccacattcgc ggtaagagga gaagtcaatt gatatgtagc ctccgcatat gctgaatgga
     1441 catttttttt aacgttgtag gtatttaatt caccaacgct catggtatat tttccacttt
     1501 cgtagttggc ctggttatat aaatcatcca attgcaggtt atctatgggg aatgttcccg
     1561 aaagagcgtc aaaattgtac tctacagcct caaacttgtc atcaacaaag cgtccattgt
     1621 aacctatttc cagggcagag ttcgtattgt caaattcatc attcagttta tactgaagat
     1681 tcacttttgt atttatatca ttttctttca gttcagaaaa gaaacgtttt tgccggttac
     1741 ttcccgttaa aatataggta ccatcatcct gttttgaaag ataattttcg cgacggtcag
     1801 gttccagccc ttttatcctg ttatacgatg ccccggcgtt tagtttcatt ttgttcgata
     1861 atttccagct ggaaataagt tgatgcgtga atagtaaatt gtcgttcgtt tgttgtctga
     1921 ttgcccttcc cgtgtaatcg ttactgtctt gatggctttc ggtatgtttt ccgctgtatt
     1981 gcccaacgta ttgattattg gcatgaacca tcatgaaatt atacgccagg ttgtgcgctt
     2041 tgttaatccc gaaattgata tttccgagaa caagctggtt aatgttttgg gaatacttgt
     2101 cgccttgctg gtcttgataa atggttccgt cggaagtggt gtttctgatc aattcccggg
     2161 tataagaata atttgttccg tgtgagccta caacaaaaaa tgaaagtgga tgattatccg
     2221 ctcccacttt aaattgtttt cctccggaga ttccaaaact gtggttcacg ggtgtcttta
     2281 tgaccgttgg atcgaggctg ttggcaaaat aaaccttgtt ttcgttcggt tttttattgt
     2341 tggcaaatcc caagtagttt gagccgtcct gacgtaggaa gtcaactccg atagtccccc
     2401 ggttcaatcc tcccgatatt tcggcaccaa aagcccagtc attaatcaat tcttttgacg
     2461 aaatgtctat gatagcgccc cctgcatctc cggtagagct acccgaaaaa actttgttta
     2521 cccctacatt ttgaatgata tcggttccga aaaagtcgag tgcgatgttt ttgtattcgg
     2581 gatcttccga agggacaaga aaaccgttga gggttgtcgc gttgtatcgg tcgcccaagc
     2641 cccgcacaaa aacatttttc acgccttcct gtttggatat tcccgaaatt ttggacacgg
     2701 ctgcttccgc atttccaatc cccttgcgtg ataactctct tgcccctaca gcttgcgtgg
     2761 caagtaatgc ttttttttgc tcgagaagca gaatattctc gctttcgcgg ttagcgcgtg
     2821 cgaccacttc aaccgcttcg aggatgatat cgtcggagtt catttcgaaa tccaccaccg
     2881 tttcgcggtt tgtttctact gcaatccccg ttttagttac cggttgatat gccacgtaag
     2941 atgcaacgag cgtgtgcgtt cccgccgata cgttattgat aacgaaattg ccgtccagat
     3001 cggcggcagc cccgtttgtg gtttcttcga ccaaaaccga agctcctatt agtggttctt
     3061 tggtcttggc atcaacaatt gttcctttga ttgttcccgt ctgagcataa atagcggatg
     3121 ctgaaacgaa aaataaaagt aaaacagata acctaaacaa tacctttgct ttacttatca
     3181 ttaattttga gtcttttttc attaacgagt atcataattt atcgtcacaa aggtcaagta
     3241 taaatgttac aaagcagtta cattctgatt acattattgt taagttttca ccaataaaaa
     3301 aaccgatact ggtgtaccgg tttttccaac ttactcatgt gctgttactt ctattgtact
     3361 attttcatta taggctctcc ggcctgaacg atatcacccg gttttacata tatcttatac
     3421 acttttcccg aatattcaga aattacgtcg ttctccattt tcatagcttc cagaacaaac
     3481 aggttttttc ccctttgcac ctcatcgcct tcttttacca gaaacttcat aacgctgccg
     3541 ggcatagggc tttctgtaat gtatccctcg ggttccttta cttccgcatc ggtaggtacc
     3601 gttccagcac cggttacagt tgttttttgc gattgatgcg atgcaaattc cgtttcacgg
     3661 attttcctca ggtattgggt ggccacggca gggaaaagtt caagtaacaa ctcctctttt
     3721 tcgtctttag ccagccggac attttcgaac tccggcaaaa caggattctc ctgacgttta
     3781 tacgtgctgg tatcgtacgg agtttcatca cgcgttccgg caattttcaa cctgaattcc
     3841 ggatctaccg gaacgggagt ttttccatat tcccccttta ctagtgcaac aaattgatta
     3901 gaagcattgg catatttatc ccgcccattc ttcagattca gggccgaact gaccgcctgc
     3961 actcctacga tctgacttgt aggagtaacc aaaggtggca atccagcatc gagccggaca
     4021 cttggaataa gtttcattgc atcttccaag atatccagcg agttaaattg tttcaattgt
     4081 gccaccatat tggtgtacat ccctccggga atctcggcgt ttttcaccct ctcgtcgggt
     4141 ttggggaaat tgaaataagc ttcaatttgg tggcaggcct ccagtaaagc cgtctcgttg
     4201 ttgatttgaa ctgcggcaat agcttcgtca aacaacttat caatagcagc aggcagggta
     4261 tccgtaagcg ggttaaacgg atttgggaac tgttttaccg tatcgaaagc ttccaattcc
     4321 cggcggattc cgaacaattc ggcatttatt tttgccacag cctccatgtt tacatccagt
     4381 tcaatgccca gtttttggca gaaaatataa acgagttcaa tggctggagc agccggcccg
     4441 ccggaaaaat accagatatt ggtgtctacg atatctgccc catttacgat tgctgagagt
     4501 acggaaccca atccataccc cggggtgcaa tgtgtgtgaa aatcgatggg aacattaagt
     4561 tctcttttga ataatgagat aaggtttgcc acacgcgtgg gcggaatcag tccactcata
     4621 tctttaatgg tgatcatgtc agcacccaac gctagcattt ccttggcttt ctgaagaaaa
     4681 tagcggtctg taaacacatt ttcttttttt atccgtattt cttctttctt gttgaacagc
     4741 cccagaaagc ctttcttttc aacaatttta acctcatcgt cgtatttggg atctatcgtg
     4801 tagcaaaccg cgcaatctgc caaaccaccg tatttcttaa tgtattttat actcgatttt
     4861 atattgtcaa catcgtttag cgcatcgaaa atacgcataa tgttgagtcc gctagagacg
     4921 gcatttttga aaaatccatc aataatttca tccggatagg gaacgtatcc atagaggttt
     4981 cgtccacgag aaagcgctgt gagttttgag gctccctgca caccgtcgct gacttttttt
     5041 aacctgtccc atgggttttc gcctaaatag cgcataacgg agtctggaac agcacctccc
     5101 caaacttcca ttgcatagaa attggcatct ctgtaataag gcaacactct gtcaacttgt
     5161 gattggttca ttcgggtagc aaaagccgat tgctgaccgt ccctcaaagt gagatctctg
     5221 attaaaagtt tacgtttcat ctgtttaaaa atttaatttc tactttttat ggtggttttt
     5281 gacattagtt tcgttgtttt tcactattta tacttacagt ttttaaacat tataaatatc
     5341 tcaaatacag aaatttagtt ttatctttcg ccatacaaag taacaaatta atttcgaatt
     5401 ataagcaatt aattaaagat ttttttcttt aattcatgct aaaaacaaca aaagcagcat
     5461 ttcgtgttta aaatgctgct tccgggtgtg taaacgaaag gaaattacac cttagaaacg
     5521 ttttttccct ttcttggaga cgttgaaatc ctgttttggt ttgtcacctt tgtatttctt
     5581 gttctccggc atattgttgt ctgctaattc aaccaccagt tttctgtctt ccacgaagac
     5641 tcctttaaag gatttgagaa tttgcttgac attttcttcg gggacttcaa aaaacgaaaa
     5701 gttcttcatc agatcgatac gccctaccct gatcttcccg ggcacataat cgttgataaa
     5761 acccatcagg ttagccgggt tggcgccgtc catttttccg atattgataa aaaagcgggc
     5821 atatccattt tcagcctttt tcggtccccc ttcgtttctg tcaaattttt ttccttttcc
     5881 acgttcctct cttttcgagt tgtgttcttt gggttcttca atttcattgg cggcttggta
     5941 gtagtcaatc agccggttaa attccaagga gaccatacgt ttaatgacat cctccttttc
     6001 cagccaatca agtttcttga agatggaagg cagtaaacgg gcaatttctt cttcatttac
     6061 cttaaccttt tcaattttat ctacaaagtt gaacagttgc ttttcgcaaa taatatctcc
     6121 tttgggtacc tgacgatatt caaatttttt attgatgagt ttttctatct gagcaacttt
     6181 tcctttttct ttggtatgga taatggatat ggatgttccg gtttttccca ccctgcctgt
     6241 acgtccgcta cggtgcgtgt atacttcgta atcatcggga agcccaaagt taatcacatg
     6301 agtgatatcg tccacgtcca gtcctcgggc agcaacgtcg gtagcaataa gcaactgcag
     6361 gttccgtaag cggaacttgt tcatgacata gtcacgttgt gcctgcgaca ggtcaccgtg
     6421 caacgaatcg gcatcgtagc catcctgaat aagcttatct gctatttcct gcgtttcttt
     6481 tcttgtcctg caaaagacaa tgccgtatat gttggggtag taatccacga tccgttttaa
     6541 cgcaaggtat ttgtcttttg cgtgaaccat gaaataggtg tgtttcacgt tcaacgcacc
     6601 ttcgtttttc cgtccaatgg ttatctccat ggggttggac atatatttcc gggtaatttt
     6661 ggctatctcc tggggcatgg tggcggaaaa aagcagcatg ctgcggttgt cgggaacgtg
     6721 agacaaaatt tcatctatac tttccgtaaa tcccatgtgt aacatttcat cagcttcatc
     6781 cagcacaacg gtatggacat ttgccaacga aacggttttg cggtttatca aatcaatcaa
     6841 cctacccggt gtggcaacga taacatgaac gcctcgtttt aacgttttaa tctggctttc
     6901 tatgcttgat ccgccgtaga caggaaggat ttttatatca tcaatatatt tggaaaaatc
     6961 ggttaaatca ccggctatct gtaaacaaag ctcccgtgta ggacaaagga tcagtgtctg
     7021 cggtgcatgc agttcgatgg ctgttttttg gatgattggc aaaccgaaag cagcagtttt
     7081 tccagttccg gtttgcgcca gagcaattat gtcacgggta tttgccagca gttgcggaat
     7141 cacttcctcc tgtaccggca tgggttgttc aaaacccatt tcctgaatgg cacgccaaat
     7201 cgcgggtgc
//
LOCUS       DAOSDR010000137         7174 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrimonas sp. isolate AMR_MDS_1826
            NODE_3812_length_7174_cov_4.063913, whole genome shotgun sequence.
ACCESSION   DAOSDR010000137 DAOSDR010000000
VERSION     DAOSDR010000137.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863355
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrimonas sp. (wastewater metagenome)
  ORGANISM  Petrimonas sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Dysgonomonadaceae; Petrimonas.
REFERENCE   1  (bases 1 to 7174)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7174)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:10:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,720
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 35
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7174
                     /organism="Petrimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3812_length_7174_cov_4.063913"
                     /isolate="AMR_MDS_1826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2023866"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..232
                     /locus_tag="PLI30_06615"
     CDS             <1..232
                     /locus_tag="PLI30_06615"
                     /inference="COORDINATES: protein motif:HMM:NF018963.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1460 domain-containing protein"
                     /protein_id="HOI79260.1"
                     /translation="LESAKVDIPHMAMIAFTTSIKGLDTTHTGFAYKKDGKLTFIHAS
                     SAKNNVVIDEKTLSDYCKKQKSCTGITVMLVR"
     gene            complement(236..691)
                     /locus_tag="PLI30_06620"
     CDS             complement(236..691)
                     /locus_tag="PLI30_06620"
                     /inference="COORDINATES: protein motif:HMM:NF014373.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HOI79261.1"
                     /translation="MPTWEFVFRLLAAVAAGGAVGLERQINNKSAGFRTNTLVSVGAC
                     IYVLINVILTENGGDPTRIIGQIVTGIGFLGAGVILHRGINVQGLTTAATIWCSAALG
                     SLAGLGLYVELLISALLIILVNTAFKKADKWFMGEKKKKAGNNNDHSFE"
     gene            complement(695..1759)
                     /locus_tag="PLI30_06625"
     CDS             complement(695..1759)
                     /locus_tag="PLI30_06625"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021127.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0045227 - capsule polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HOI79262.1"
                     /translation="MRFTASTLFLFLAFSLSQAQEKQVRLIFAGDAMQHLPQIRAAQT
                     PDGYNYDSCFYLLKEKIASADIACVNFETTLAGKPYSGYPQFSAPDEFASGLKDAGFD
                     IFFLANNHVVDKGRRGVERTLGVLDSIGIKHTGVFRSKDFRALNYPLMVIKNGIRIAF
                     LNYTYDTNGLPVTAPHMVNVIDTLQMKSDLRLTQLYNPDIVIANMHWGDEYITRPNAE
                     QKRLASFLFRNGVRIIIGNHPHVVQPLAKNKTNNEIETVVYYSLGNFVSNQQKINTDG
                     GAMAEIVIHMADDNSPVKIISCSYSLIWVRKHTENGKLKYTVIPVEEHKKKAIPELTP
                     EEWQKMNIFATKADQLIGSF"
     gene            complement(1770..3443)
                     /locus_tag="PLI30_06630"
     CDS             complement(1770..3443)
                     /locus_tag="PLI30_06630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006842898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HOI79263.1"
                     /translation="MKKISFLLLIVLIATTVKAQTTQTDKNQRYFDINKSIDIFNSVI
                     RELDMFYVDSVKVDSLINGTIRTMLARMDPYTEYYAEENIGDLQFMTTGEYGGIGSII
                     SYNNNRVIINEPYKGLAADKAGLKAGDAILEIDGADMVKASVKEVSDKLKGTPGTTLK
                     LKIQRPGENKSREITLVREKIEIDPITYSAVLDDAVGYLHYSGFTNGSSNRVKETILD
                     LKKKGAESLVIDLRGNGGGILDEAVNVVNFFVQKGLEIVSTRGKVKQWDRSYHTQNQP
                     IDTLMPIVVLIDTGSASASEIVAGSLQDLDRAVIIGNRSFGKGLVQTPRDLPYGGNIK
                     ITTSKYYIPSGRCIQALDYSHRNPDGSVARVPDSLTHVFKTKSGREVRDGGGITPDYV
                     IPQEKSGTIGYYLLTENIIFDYVTDWALKHPSVAPPANFHLSDADYELFKQFVKSKDF
                     QYDQMSNRSLQSLKNIMEFEGYFNTASEEFKALEEKLQPNLDRDLELFSKEIRQMIET
                     EIVQRYYYKEGVLMYELKDDAALKKAKEVLKDKQLYARTLQPQPVTEPQ"
     gene            3619..4026
                     /locus_tag="PLI30_06635"
     CDS             3619..4026
                     /locus_tag="PLI30_06635"
                     /inference="COORDINATES: protein motif:HMM:NF037970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HOI79264.1"
                     /translation="MNNYLRHILLPVFVGFLIFTVTCLISSDDVPQLPQIIAWDKIAH
                     FGMFFVLSAVSLFDYYRLHNGRPRMSRWVFWGFILPVIYGAAIELMQKYFFSSRSAEL
                     ADFLADLLGSLTAWLIALFLYKKNRKQEKKLSL"
     gene            4090..4674
                     /locus_tag="PLI30_06640"
     CDS             4090..4674
                     /locus_tag="PLI30_06640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007571366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HOI79265.1"
                     /translation="MLKRLSFIFLFVLALVSCNPSVKKNDVNTQKQNTVQVQDTLDRQ
                     YRVKVGDPAPDFEMALPDGKKIKLSDLRGRVVMLQFTASWCGVCRKEMPHIEKEIWQK
                     HKDNPQFALYGIDREEPAEKIQVLREATGVTYPIGMDPDAGIFGLYAEKDAGITRNII
                     IDREGKIVMLTRLFEEEEFNEMVSLIDKLLRAMS"
     gene            4707..5174
                     /locus_tag="PLI30_06645"
     CDS             4707..5174
                     /locus_tag="PLI30_06645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019539700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4870 domain-containing protein"
                     /protein_id="HOI79266.1"
                     /translation="MKYEDLKILDQLREKGSITEEEYQREKAKILNETISSSTSSGSK
                     PLFGLEENTYLMLMHVSQLLGLLIPLAGFVAPVLMWVINKDINANVDLHGKNILNFTI
                     SYLIYSAVLAITIIGIPLLVVLGIVYLVFVILAAVKANNGEYWRYPFIIQFLK"
     gene            5174..5860
                     /locus_tag="PLI30_06650"
     CDS             5174..5860
                     /locus_tag="PLI30_06650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005854551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOI79267.1"
                     /translation="MSDDFLIKYTGVQLNREENIILRDVNLEVNRGDFLYIIGKVGSG
                     KSTLLKSMYAEIPIESGDARVFDYHLQALKRKQIPYLRRKIGIVFQDFQLLIDRTVEK
                     NLEFVLRATGWKDKIIIKDRIIEVLTQVGMQNKTYKMPHELSGGEQQRVVIARALLNS
                     PLLILADEPTGNLDPETGSQIVDLLQQISSGGTAVIMSTHNYSIVQTFPGRIMKCENT
                     ELVDLKNFGY"
     gene            5988..7148
                     /locus_tag="PLI30_06655"
     CDS             5988..7148
                     /locus_tag="PLI30_06655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008145918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 99-like
                     domain-containing protein"
                     /protein_id="HOI79268.1"
                     /translation="MAVTSCTDKKNTVKESSNNSEYYVAAYIWPSCHHDERFGDMLWP
                     EGTGEWEVIKKGNPRFEGHYQPRLPLWGYEMDNDPKVVEKWIDLAVEHGVNVFVYDWY
                     WYDEGPFLESALNDGFLKAKNNSKMQFYIMWANHDVKRNYWNVHKYKDDTSVLWNAVV
                     DENNFKIIVDRVINQYFKQPNYFKIDGKPVFSIFSIDKLMESFGGDAKRTREALDYFR
                     EEVKKAGFPDLHIQWNQGGGSLMSKESAEKFSNKVEEMGFNSVAMYNMGGLNEDYLVY
                     GDNSRKIRTQMDSILNVPLFPCVSVGWDDTPRFPAKGMKDVVHYHNTPESFAALLYQA
                     KKYADSHPEQTKLITINAWNEWVEGSYLLPDMQNGFGYLKAVKEVMSGEYEP"
BASE COUNT         1963 a         1474 c         1667 g         2070 t
ORIGIN      
        1 gttagagtca gcgaaagtag atattccaca catggcgatg attgccttta ccacctccat
       61 caaagggttg gacaccacac acacggggtt tgcctacaaa aaggacggta aactgacgtt
      121 tatccacgca tcgagtgcga aaaacaacgt ggtgattgat gaaaaaacgt tgagtgatta
      181 ttgtaagaag caaaaatcct gtacgggaat cacggtaatg cttgtccgtt gatttctatt
      241 cgaatgaatg atcgttgtta tttccggctt tctttttctt ctctcccata aaccatttgt
      301 ccgctttctt gaaggccgta ttgaccagga taatcaacag ggcggaaatc aacagctcca
      361 catacaaacc caatccggca agggagccca gggcggcgct gcaccatatc gtagccgcag
      421 tggtgaggcc ctggacattg atacccctgt gcaggatcac acccgctccc agaaagccga
      481 ttcccgttac gatctggccg attatccggg tagggtctcc gccgttttcg gttaaaatta
      541 cattgatcag cacgtaaata caggcaccta ccgataccag ggtattggta cggaatcccg
      601 cacttttatt attgatttga cgttcaagtc ccacggcgcc accggcagca actgccgcca
      661 gcaggcgaaa aacaaactcc catgttggca tagcttaaaa cgatccaatt agttgatcgg
      721 ccttggttgc aaagatattc attttttgcc attcttccgg ggttagctcc ggaattgcct
      781 tttttttgtg ttcctctacc ggaatgaccg tgtatttcaa cttcccgttt tcggtatgtt
      841 ttctcaccca gatcaacgag taactgcacg atattatctt caccggactg ttatcatccg
      901 ccatatggat aactatctca gccattgccc cgccgtcggt atttattttt tgctggttgg
      961 agacgaaatt accgagcgaa taataaacaa cggtctcaat ctcgttgttt gttttattct
     1021 tagccaacgg ttgtactacg tgcggatgat tgccgataat gattctgaca ccgttcctga
     1081 aaagaaaact ggccagcctt ttctgttcgg cattcggacg ggttatgtat tcatcgcccc
     1141 aatgcatatt ggcgataacg atatcggggt tataaagttg cgtgagcctt aaatcgcttt
     1201 tcatttgcag cgtatcgatg acgttcacca tatggggcgc agtaaccggg agtccgttgg
     1261 tgtcgtacgt gtagttcagg aaagcaatcc ggataccgtt tttaataacc ataagagggt
     1321 agttcaatgc ccggaagtct ttcgaacgga atactcctgt atgttttatc ccgatggaat
     1381 ccagcacgcc gagtgtgcgc tcaactcccc tgcgcccttt atcaacgacg tggttgttgg
     1441 ccagaaagaa aatatcaaat cccgcatctt tcaatccgga agcaaactcg tcgggagcac
     1501 taaattgtgg atatcccgaa taaggttttc cagctaatgt ggtctcgaaa ttcacacaag
     1561 cgatgtctgc agacgctatt ttctccttca gcaaataaaa acaagaatcg taattgtatc
     1621 cgtcaggggt ttgtgctgca cgtatttgcg gcaggtgttg catagcgtct ccggcaaaaa
     1681 tcaaccttac ctgtttctcc tgggcttgtg aaagtgaaaa ggcaaggaac agaaataaag
     1741 ttgaagcagt gaagcgcacg gcaagtgggt tattgaggtt cggttacggg ttgcggctgg
     1801 agcgtgcgag cgtaaagttg tttgtctttc agcacttctt tggccttctt cagtgctgca
     1861 tcatccttca gctcatacat cagtacacct tctttgtaat agtaccgctg aacaatctcg
     1921 gtttcgatca tctgcctgat ctctttactg aaaagttcca ggtcacgatc gaggttgggt
     1981 tgcaattttt cttccagtgc cttgaattct tccgaagctg tgttgaaata tccctcgaac
     2041 tccatgatgt tttttaacga ctgtaacgag cggttgctca tttggtcgta ctgaaaatcc
     2101 ttgcttttta caaactgttt aaacagttcg taatcagcgt cggaaagatg aaaattggcg
     2161 ggaggtgcaa cggacggatg ttttagggcc caatcggtga cgtagtcgaa aataatgttt
     2221 tcggtaagta aataataacc gatcgtgccg cttttttcct ggggaatgac gtagtcaggg
     2281 gtgataccgc cgccatcacg cacttcacga ccgcttttag ttttgaaaac atgcgtcaaa
     2341 ctatccggaa cacgtgctac ggaaccatcg ggattgcggt gcgaataatc cagggcctga
     2401 atacaacgtc cgcttggaat gtaatacttc gatgtagtga ttttgatgtt tccaccataa
     2461 ggtaaatcac gtggtgtctg caccaagcct tttccgaagg agcgattgcc gataataaca
     2521 gcccggtcca aatcctgaag tgagccggca acaatttcag aggcagaagc cgagccggta
     2581 tcgataagca caacaatcgg cataagtgta tcgatcggtt ggttttgagt gtgatacgat
     2641 ctgtcccatt gttttacctt gcctcttgtg gaaacaatct caagcccttt ctgcacgaag
     2701 aagttgacaa cgttgacggc ttcatccaaa atacctccac cgtttccgcg caggtcaata
     2761 accagagatt cagcgccttt tttcttcaga tccaggatcg tttccttaac ccggttcgag
     2821 ctgccgttgg taaagccgga ataatgcaaa tatcccacgg catcatccaa aacggcagaa
     2881 taggtgatgg ggtcgatttc tattttttcg cgaacaaggg ttatctcgcg ggatttgttt
     2941 tctccgggac gttgaatttt cagtttcaac gttgttccgg gagttccttt taacttatcg
     3001 ctgacctctt ttacggatgc ttttaccata tccgctccgt caatttccag aatggcatcg
     3061 cctgccttta ggcctgcttt atcggccgca agtcctttgt agggttcgtt gataatcacc
     3121 cggttgttgt tgtaggaaat gattgaccct atgccgccat attcgcctgt agtcataaat
     3181 tgaagatcac cgatattctc ttcggcatag tactccgtat agggatccat ccgggccagc
     3241 atggtgcgga tggttccgtt gatcaaactg tctaccttta cgctgtccac gtagaacata
     3301 tccaattccc tgatcacgga gttgaaaata tcgatgcttt tgttgatatc gaaataccgt
     3361 tgatttttat cggtttgcgt ggtttgggct ttaacggttg ttgcgattaa tacgattaat
     3421 agaagaaaag aaattttttt catatttaaa gaaatccgaa aatgatcgtt ttgtatgttg
     3481 ttttttgtat ttagctgaca aagaaacgaa tttttcagga atttaaggga ttgttttagt
     3541 tatatgtttt taaaattgag cgtggagtca tgtttttaaa ttatctttga gacgtttttt
     3601 gcaaaacgat gtccctatat gaataattat ctccgtcata ttcttcttcc cgtttttgtt
     3661 ggattcctga ttttcaccgt aacctgcttg attagttctg atgatgtccc tcaacttcca
     3721 caaattatag cttgggacaa gatagctcac ttcggaatgt tttttgtatt gtcggccgtc
     3781 tccttgtttg attattatcg cttgcataac ggtcgtccca gaatgagccg ctgggttttc
     3841 tggggattta ttttacctgt tatatacgga gcagctattg aactgatgca gaagtacttc
     3901 tttagctcac gcagtgcaga actggccgat tttttggccg acctcctggg ttcccttacc
     3961 gcttggctta tcgctttatt tttatacaaa aaaaatcgga aacaggaaaa aaaactatct
     4021 ttgtgaagtg taaacgaaga acaaagcgtt tagcgtaaaa gatttatttt tgaaaaccgt
     4081 taaaatcata tgcttaaaag actatctttc atttttttat ttgtcctggc gttggtatcg
     4141 tgtaaccctt ccgtgaaaaa gaacgatgtt aatacgcaaa aacaaaatac cgttcaggtt
     4201 caggataccc ttgaccgcca ataccgggta aaagtaggag accctgctcc cgattttgag
     4261 atggctttgc ccgacggtaa aaaaataaaa ctatccgatt tgcgcgggag agtggtgatg
     4321 ctgcaattta ccgccagttg gtgcggggtt tgtcggaaag aaatgccgca catcgagaaa
     4381 gagatatggc aaaaacacaa agacaatccc cagtttgcgt tgtatggtat tgaccgggag
     4441 gaaccggcag aaaaaattca ggtgctgcgt gaggcaaccg gtgttaccta tcccattgga
     4501 atggatccgg atgcgggaat cttcgggttg tacgccgaaa aagatgccgg catcacgcgc
     4561 aatatcatta ttgacaggga gggtaagatc gttatgctta cccggttgtt tgaagaagag
     4621 gaattcaacg aaatggtttc cttgattgac aagttgctga gagctatgag ttaagattaa
     4681 atgtactaca taacacaaaa aagatcatga aatacgaaga tttaaaaatt ctggaccaac
     4741 ttcgtgaaaa aggaagcatc accgaagaag aataccaacg tgaaaaagcg aagatattga
     4801 atgaaacaat ttcgtcgtcg acatcgtcgg gcagtaagcc tcttttcgga ctggaagaaa
     4861 acacttacct catgttgatg catgtttccc aattgctggg tttgctgata ccgttagctg
     4921 ggtttgtggc gcccgtactc atgtgggtta tcaataagga tatcaacgcc aacgtggatc
     4981 ttcacggaaa aaatatattg aattttacga tcagctatct catttacagt gcggtactag
     5041 ctattactat tatcggtatt ccgttgctcg tcgttctggg tattgtttac ctggtttttg
     5101 ttattttggc tgctgttaaa gccaataacg gcgaatactg gagatatccg tttattattc
     5161 aatttttaaa ataatgagtg acgatttttt gataaaatat accggagtac aactcaacag
     5221 agaggaaaat ataattttac gtgatgtgaa cctggaggtc aaccggggtg attttttata
     5281 cattatcggt aaagtagggt cggggaaaag tacattactg aagagtatgt atgctgaaat
     5341 tccgattgag tccggcgatg cccgtgtctt cgattaccac ttgcaagcct tgaaaagaaa
     5401 acagatccct tacctgcgta gaaagatcgg tatcgttttc caggattttc agcttctcat
     5461 cgaccgtacg gtagaaaaga acctggagtt tgtgctccgg gcgacgggtt ggaaagacaa
     5521 aataataatt aaggaccgta ttattgaggt attgactcag gttggaatgc aaaacaaaac
     5581 ctacaaaatg ccgcacgaac tttcaggtgg cgaacagcaa cgtgtggtaa ttgcccgggc
     5641 gttgctcaat tcgccattgc tgattcttgc cgatgaacct acaggaaatc tggatcccga
     5701 aaccggtagc cagattgtag acttactgca acagatttcc agtgggggca ctgctgttat
     5761 tatgtcgacc cacaattatt ccattgttca gacttttccc ggacgtatca tgaaatgtga
     5821 aaacacggaa ttggtcgatt tgaagaattt cggctattag agaattgtta ggaggattct
     5881 caatagtttt tactataatt gcaaaaatat tttttaactc aaatactatg gttttcaaaa
     5941 ttaatgtaaa ttttttttta aagttgtccg tatggatggc atgctgtatg gcagtaactt
     6001 catgtaccga taaaaaaaac acggttaagg agagcagtaa taattctgag tattacgttg
     6061 cagcctacat ttggccatcg tgccatcacg acgagcgttt cggtgatatg ttgtggcccg
     6121 aaggaaccgg ggaatgggag gtgatcaaga aaggaaaccc acgatttgaa ggccattacc
     6181 agccgaggct tccgttgtgg ggttatgaga tggataacga ccctaaggtg gttgagaaat
     6241 ggattgacct ggcagtggaa catggagtca atgtctttgt gtatgactgg tactggtatg
     6301 acgaagggcc atttctggaa agtgcgctga acgatggatt cctgaaagca aaaaataact
     6361 cgaagatgca gttttatatc atgtgggcca atcacgatgt aaagcgcaat tattggaatg
     6421 ttcacaaata taaggacgat acgtcggttt tgtggaacgc tgttgttgat gaaaataatt
     6481 ttaagattat cgttgaccgg gtgatcaatc aatatttcaa gcaaccgaat tattttaaga
     6541 ttgacggcaa gcccgttttc tctattttca gtattgacaa gctgatggag agcttcggag
     6601 gagatgcgaa gagaacacgg gaagcactgg attatttccg ggaggaagtt aaaaaagcgg
     6661 gattcccaga cctgcatatt caatggaacc aggggggagg ctccctcatg tcgaaagagt
     6721 cggctgaaaa attttcaaac aaagtggagg aaatgggatt caacagtgtt gccatgtata
     6781 acatgggagg actcaacgaa gattatttgg tatacggcga caattccaga aaaatacgga
     6841 ctcaaatgga ttccatattg aacgtaccgc tattcccatg tgtatctgtt ggctgggatg
     6901 atacgccgcg ttttccagcg aaaggcatga aggatgtagt tcattaccac aacacccccg
     6961 aaagcttcgc agctttgttg tatcaagcta agaagtatgc tgatagtcat cctgaacaaa
     7021 cgaaactcat taccataaat gcctggaacg aatgggtgga ggggagttac ctcctgcctg
     7081 atatgcagaa tggatttggc tatttaaaag ccgtaaaaga ggtgatgagc ggggaatacg
     7141 agccctaaaa gtacccgcta ccggttcaaa ttca
//
LOCUS       DAOSDR010000138         7150 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrimonas sp. isolate AMR_MDS_1826
            NODE_3823_length_7150_cov_3.590416, whole genome shotgun sequence.
ACCESSION   DAOSDR010000138 DAOSDR010000000
VERSION     DAOSDR010000138.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863355
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrimonas sp. (wastewater metagenome)
  ORGANISM  Petrimonas sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Dysgonomonadaceae; Petrimonas.
REFERENCE   1  (bases 1 to 7150)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7150)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:10:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,720
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 35
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7150
                     /organism="Petrimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3823_length_7150_cov_3.590416"
                     /isolate="AMR_MDS_1826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2023866"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..280
                     /locus_tag="PLI30_06660"
     CDS             <1..280
                     /locus_tag="PLI30_06660"
                     /inference="COORDINATES: protein motif:HMM:NF023096.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF3267 domain-containing protein"
                     /protein_id="HOI79269.1"
                     /translation="MAPFLTPYCHCTEPLKIRHYLFGALTPAVILGLGPAITGLFIGK
                     YLVTFLGIVFIVSAIGDLMIAHLLWEEKPTDYAQDHPSEAGCFVFRKR"
     gene            350..1384
                     /locus_tag="PLI30_06665"
     CDS             350..1384
                     /locus_tag="PLI30_06665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI79270.1"
                     /translation="MRKLIFPLVLILLFGFIACKGRSDSSAKSADTPPFSTLENIEKE
                     DIKNQKPVDQAEYNDLLNYIANGDTTGLWPVQNQPLPLEGAVLPYHRVVAYYGNLYSK
                     NMGVLGEYPPEEMWSRLLKETEAWEQADPKTPVVPAIHYIAVVAQGTPWSDGKYRYRM
                     PQQQIDSALAIAKMHDALVFLDVQVGLSTVELEIPQLEKYLLMPHVHLGIDPEFSMKD
                     GTPPGKKIGTLDAEDINFCSAYLAELVQDYNLPPKILIVHRFTKGMVTHYKNIKLSPE
                     VQIVINMDGFGRPELKYSTYNRFIHPEPIQFTGFKLFYKNDTKESPNHLLTPEELMKL
                     QPRPVYLQFQ"
     gene            1492..2406
                     /locus_tag="PLI30_06670"
     CDS             1492..2406
                     /locus_tag="PLI30_06670"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019537959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase"
                     /protein_id="HOI79271.1"
                     /translation="MNYSKTFQEIYNELQRVEDIGQVANYIPELAHVNPNQFGVHLIT
                     VNGEYFAFGDADVKFSIQSIAKVFSFVLAYSRVKSNIWERMDLEPAGTPFNSLVQLEY
                     DKGIPRNPFINAGAIVVCDILVDELASPRENVLSFIRSLTKSSTIDYNPLVAHSEKKS
                     GFRNYALVNLMKAFGNIKNDIEKVMDLYFSICSLEMNCKELSESFLFLANNGIVPHTG
                     EKILSPSRTKRTNALMQTCGFYDEAGQFTFKVGLPGKSGVGGGIVAVHPEKYAIAVWS
                     PRLNKKGNSYKGMLFLEEFTTKTKLSIF"
     assembly_gap    2584..2593
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            2644..3534
                     /locus_tag="PLI30_06675"
     CDS             2644..3534
                     /locus_tag="PLI30_06675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019537960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI79272.1"
                     /translation="MKIDFKERTPQLIAIIGVLSLILAIAIIYIVSKNRQINTMEQQF
                     TVDKQELEDEYEAISMQYEGFKFSVQNDSLLYKLENEQAKVQRLQEQLRMTDAANKAE
                     IKRLKDELATLRKVLKSYVQQIDSLHRLNTELQAKNEQITKQYQQTSRTLNQVSQEKE
                     QLSEKVTLASKLDATGVSVKAVNDRGREQKRLSRSSQFVVSFLITKNITAEPGERIIY
                     VRIMSPDGGVLTKNPGSTFPYENGNLQYSMKRIVEYGGEEIPVSMYWDIEEFLMPGTY
                     KADIFADGSLIGSRSFSMEE"
     gene            3732..4292
                     /locus_tag="PLI30_06680"
     CDS             3732..4292
                     /locus_tag="PLI30_06680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI79273.1"
                     /translation="MCIIAATAASKLNAQEFNDEMKKKLRQSLTVPEQKPIQYQPKRA
                     NIQVLPQDNQEVLKVSPATKLPTKYDKIQVLHPPEEYEVHLNLNVTNIGTRQLNRAKI
                     DYSNGKFNAVPDARSITQWVQYTRGDYGTGISIGEGSAEWLYKLRKIIPPYSGFDADP
                     IRTIQNYKARKRKEKVDRIKKAYGQE"
     gene            4405..5811
                     /gene="uxaC"
                     /locus_tag="PLI30_06685"
     CDS             4405..5811
                     /gene="uxaC"
                     /locus_tag="PLI30_06685"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005654141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008880 - glucuronate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HOI79274.1"
                     /translation="MKRFIHKNFLLQTDTARELYHEHAKKQPIIDYHCHLDPAHIAAD
                     RKFDNLGQIWLEGDHYKWRAMRTNGIDERYCTGKDTSDWEKFEKWAETVPYTMRNPLY
                     HWTHLELKTAFGVEELLNPESARRIYDTCTEKLRTPEFSARGLMKRYDVEVVCTTDDP
                     ADTLEHHIALKNEGFEIKVLPTWRPDKAMAVEKPTEYRAYVEKLSEISGINIINFNNL
                     IEALRNRHDFFASAGCKLSDHGVEEFYAEPYTTAEIENIFDKVYGGSELSKEEVLKFK
                     SAMLYEGAVMDWEKGWTQQFHYGAIRNNNTRLFNQLGPDTGFDSIGDFNVAKAMSRFF
                     DQLDKNNKLAKTIIYNLNPKDNDMLATMIGNFQDGSVAGKMQFGSGWWFLDQKTGMEA
                     QINSLSNLGLLSRFVGMLTDSRSFLSYPRHEYFRRILCNLIGNDIENGLLPRSEMDLF
                     GQMVENISYYNAKKFFDF"
     gene            5835..7082
                     /locus_tag="PLI30_06690"
     CDS             5835..7082
                     /locus_tag="PLI30_06690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019538272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate epimerase family protein"
                     /protein_id="HOI79275.1"
                     /translation="MKLEKFSIGTGDRFSHQGEAQLRAIIKANSKGVNISPVWNKSNR
                     EHIYVHSKPEDVRKEADSAAQNLNFTGKYFVDADHINLNTVGPFVASADFFTLDVASF
                     IGKQSSEEEVNRFLSSCKKYLGKLSIPGMSMPLQITEDLLRNIACKFLAATQQAADIY
                     TFLKNEKGANNFITEVSMDEVESPQTPVEMLFILKMLADKGVPAQTIAPKFTGRFNKG
                     VDYVGDLTQFEKEFEEDVLVIDFAVKEFGLPEELKLSVHSGSDKFSIYPIMAKIIRKY
                     DKGLHLKTAGTTWLEEVIGLAVAGGEALALAKKIYAGSYNRKDELCAPYADVIDIDST
                     KLPSVEEVNRWSSEKFANTLRHIPGHPDYNSNFRQLIHVAYKVAAELENEYTDALKQY
                     ADIIGSCVEENIYDRHLKRLFNL"
BASE COUNT         2304 a         1566 c         1472 g         1798 t
ORIGIN      
        1 aatggcgcca tttcttaccc cctattgcca ttgcacagag ccgcttaaaa taaggcacta
       61 ccttttcgga gcgctcactc ccgctgttat tcttggattg ggccctgcca tcacaggctt
      121 atttattggt aaatatttag taacattttt gggcattgtt tttatcgtat ctgctattgg
      181 tgatcttatg attgcccacc tgctgtggga agaaaagcct accgattatg cccaagacca
      241 tccgtcagaa gcaggatgtt tcgttttcag aaaaagataa taccctacaa aaaaatcaat
      301 ttacgattga attttgaata tataaaccta tgcattaaat tattactgaa tgagaaaatt
      361 aatcttccct cttgtattaa tcctattatt tggttttatt gcctgcaaag gcagatcgga
      421 ttcatccgct aaaagtgctg atacccctcc gttttctact ttggaaaaca tcgaaaaaga
      481 agatataaaa aatcaaaagc cggtggatca agccgaatac aatgatttac ttaactacat
      541 cgccaacggc gacacaaccg gcttatggcc tgtacaaaac caaccgttgc ctcttgaagg
      601 agctgtttta ccttatcacc gggtagtggc ttactacgga aacctctatt cgaaaaatat
      661 gggtgttttg ggagaatatc ctcctgaaga aatgtggagt cgactgctta aggaaacgga
      721 agcatgggaa caagctgatc cgaaaacacc tgtcgtacct gccattcatt acattgccgt
      781 agttgcacaa ggaacgccct ggagtgacgg aaaataccgt taccggatgc cgcaacagca
      841 gatcgattct gcgctcgcta ttgccaaaat gcacgatgcg ctggtttttc tcgatgtcca
      901 ggtgggatta agcacggtgg agctggaaat tccgcaattg gaaaagtatc tgctgatgcc
      961 gcacgtacat ttgggaattg atcccgaatt ttcgatgaaa gacggcacgc ctcccggcaa
     1021 aaaaattggc actttagatg ccgaagacat taatttttgc tctgcatacc tggctgaact
     1081 tgttcaggac tacaaccttc cacctaaaat attgatcgtc catcgcttca cgaaagggat
     1141 ggtaacccat tacaaaaaca tcaagcttag tcccgaagta cagatcgtaa tcaatatgga
     1201 cgggttcggc cgccccgaac ttaaatacag tacgtataac cggtttatcc accctgaacc
     1261 tatacagttc accggtttta aacttttcta taaaaacgac accaaagaat caccgaatca
     1321 cctgctaaca ccggaggaat tgatgaagtt acaaccaagg ccggtatatc tccagtttca
     1381 gtaaagtgat ttacgacatg tatgtttcat gtcctgttga acaacctctt gttgctattg
     1441 tcaaacgcaa taatgaacat tgaacgttaa accctaaaat taaccgttaa tatgaattac
     1501 tcaaaaacat tccaggaaat ctacaacgaa cttcaacgtg ttgaagacat tggccaggtg
     1561 gctaattaca ttcccgaact ggctcacgtt aacccaaatc aatttggtgt acacctgatt
     1621 acggtcaatg gtgaatattt cgctttcggt gatgccgatg tcaagttttc cattcagagc
     1681 attgccaaag tattctcatt cgtacttgcc tactcccggg tgaaaagcaa tatctgggaa
     1741 cggatggacc tggaacctgc aggaacacct ttcaactcac tcgtacaact ggaatacgac
     1801 aaaggaattc cgcgaaaccc gttcattaat gcaggagcca ttgtggtgtg tgatatttta
     1861 gtcgacgagc ttgcttctcc tcgggagaat gtgctttcat ttatccgtag cctaacaaaa
     1921 agcagcacca tcgattacaa cccccttgtt gcacattccg aaaaaaaatc cggatttcgc
     1981 aactacgcac tcgttaacct gatgaaagct tttggaaaca tcaaaaacga catagaaaaa
     2041 gtgatggatc tctatttcag catctgctcc ctcgaaatga actgtaaaga gttatcggaa
     2101 tcttttctgt ttcttgccaa taatggcatt gtgccgcaca cgggagaaaa aattttgtcg
     2161 cccagtcgta ccaaacgtac caatgcactc atgcaaacct gcggcttcta cgacgaagcc
     2221 ggccagttta ctttcaaagt aggactgccc ggaaaaagcg gcgttggagg tggcatcgta
     2281 gccgtgcatc ccgaaaaata tgcaatcgcc gtttggagtc cgcgcctcaa caaaaaagga
     2341 aactcctaca aaggaatgct ttttctggaa gaatttacaa caaaaactaa actgtcaatt
     2401 ttttaataac acaaagaagc ctaaagggca acagccaaca ataaatctta aatttataaa
     2461 aatcatcttt taacaattgt ctttttatat gaaaaacctt atttttgtgc acaaatacag
     2521 aattttcatt tttttatgaa gcaagcatgt aaattgaaca tctcccgtat aaatggatcc
     2581 acgnnnnnnn nnncgggatg cgaggcacac acgactttca tgaaaaaata aaagaacaaa
     2641 tgcatgaaaa tagattttaa agaacgtacc ccgcaattaa ttgccatcat cggggtctta
     2701 tcactgatat tagccattgc cattatctac attgtcagta aaaacaggca gataaataca
     2761 atggaacaac aattcaccgt tgacaaacag gaacttgaag atgagtacga agctatttcc
     2821 atgcaatacg aaggattcaa gttcagtgtt caaaacgatt cactgcttta caagctggaa
     2881 aacgaacagg ctaaagtaca acggctacag gaacaactgc gcatgaccga tgcagccaac
     2941 aaagccgaga taaaacgact gaaagacgaa ctggcaacct tgcgcaaagt gctcaagtcc
     3001 tatgtccagc aaattgattc actacacagg ttaaatactg aactgcaggc caaaaacgaa
     3061 caaatcacaa aacaatatca gcaaaccagc cgcaccttga atcaagtgtc gcaagaaaaa
     3121 gaacaattaa gtgaaaaagt caccctcgct tccaagctcg atgctacggg agtaagcgta
     3181 aaagcagtga atgaccgcgg cagggagcaa aaacggttga gccgcagctc gcagttcgtt
     3241 gtttcgtttc ttattaccaa aaatattact gccgagcccg gcgaaaggat catctatgtt
     3301 cgcataatgt cgcccgatgg cggtgtgttg acaaaaaacc cgggcagtac cttcccttac
     3361 gaaaacggca acctccagta ctccatgaaa cggatcgtgg aatatggcgg tgaagagatt
     3421 cccgtttcca tgtattggga tatcgaagag ttcctcatgc ccggaactta caaagccgat
     3481 atctttgctg acggtagcct catcggttcc cggagctttt ctatggagga ataattacaa
     3541 cagaaaagtc ctgaggaaca ggaggataag caagacaacg gatcagaact ttgtgaaact
     3601 taacttttta cggcaaatat ttaacaatct tttgtgcagc gtttttcgct aaaatgcgtc
     3661 ttactgttgt gtagaaccgt aattaacgct ttagcatcgg gcaaacgaca aaaataacgt
     3721 tcctcttttc tttgtgcatt attgccgcga cagcagcatc aaaactcaac gcgcaggaat
     3781 ttaacgacga aatgaagaaa aaacttcgtc aatcgctaac tgtgcccgaa cagaaaccga
     3841 tacaatatca acccaaacgc gcgaacattc aggtattgcc gcaagataac caggaagtac
     3901 taaaagtttc tcctgccaca aaattaccca ccaaatacga taagattcaa gtactgcatc
     3961 cgccggaaga atacgaagtt catctcaact tgaatgttac aaacatcggt acccgtcaat
     4021 taaaccgtgc aaaaattgat tattccaatg ggaaattcaa tgccgttcca gatgcgcgca
     4081 gcattacgca atgggtacaa tatacccgtg gagattacgg taccggaatt tctatcggcg
     4141 aaggttctgc cgaatggctg tataaactca gaaaaatcat tcctccctac tccgggttcg
     4201 atgctgatcc catacgcacc atccagaact ataaagcccg aaaacgaaaa gagaaagtgg
     4261 acagaataaa gaaagcgtac gggcaggaat gatccatttt ttattatcct cctccttgcc
     4321 accttaaaat aaatgattaa ttttggaaaa ttaaatctct tttatgagat tgactgtgta
     4381 cgtaaacaaa taatatatcg ccgtatgaaa agatttatcc ataaaaattt tctcctgcaa
     4441 acggatactg ccagggaatt gtaccacgaa cacgcaaaaa agcaaccgat catcgattat
     4501 cactgtcacc tggatcccgc gcacattgcc gctgaccgga aattcgacaa cctagggcaa
     4561 atctggcttg agggcgatca ttataaatgg cgtgccatga ggaccaacgg aattgacgaa
     4621 cgctattgta caggtaaaga cacgtcggat tgggaaaaat ttgaaaaatg ggctgaaacc
     4681 gtaccctaca ccatgcgcaa cccactctat cactggaccc acttggagtt gaaaacggct
     4741 tttggtgtgg aagaattact taacccggaa tcggcaagaa gaatctacga tacgtgtacg
     4801 gaaaaactca gaacccctga attctctgct cgagggctaa tgaaaagata cgacgtcgag
     4861 gtggtttgca caaccgatga ccccgccgat acgctggaac atcacatagc tttaaagaac
     4921 gagggttttg aaattaaggt cctgcctacc tggcgaccgg acaaagccat ggccgtagaa
     4981 aagccgaccg aatacagggc ttatgttgaa aaattatccg aaatatcggg gatcaacatt
     5041 atcaatttca acaacttaat tgaagcacta agaaatcgtc acgatttctt tgcatctgcc
     5101 ggctgtaagt tatccgacca cggagtagag gagttttatg ccgagcctta cacgacggca
     5161 gaaatagaaa acattttcga taaagtgtat ggaggaagtg aattatcaaa ggaagaagtt
     5221 cttaaattca aatcggctat gctctacgaa ggggctgtta tggattggga aaaaggctgg
     5281 acacagcagt tccattatgg agctatccgc aacaataaca cccgattatt taatcaatta
     5341 ggacctgaca caggattcga ctccattggt gatttcaatg tagcaaaagc catgagtcgc
     5401 ttttttgacc agctggataa aaacaataaa ttggccaaaa ccattattta caatctcaac
     5461 ccgaaggata acgacatgct ggcgaccatg atagggaatt tccaggacgg ctccgtggca
     5521 ggaaagatgc agtttggttc agggtggtgg ttcctggacc aaaaaacggg catggaagca
     5581 caaataaatt cattatccaa cttggggctg ttaagcaggt ttgtagggat gcttaccgat
     5641 tcgcgcagtt tcctctccta tccccggcac gaatatttcc gccgtatatt gtgcaacctt
     5701 atcggaaatg acatcgaaaa cggtttattg cccagatcag aaatggatct ttttggacaa
     5761 atggtagaaa acatctctta ttacaacgca aaaaaattct ttgactttta ataaaaacat
     5821 gaacatataa ccgcatgaaa ctagaaaaat tcagtatcgg aaccggtgac cggttttctc
     5881 atcagggaga agcacagttg agagccataa taaaggcaaa cagcaaagga gttaacatta
     5941 gtcccgtatg gaataaatcg aacagggagc acatttacgt acattcaaag ccggaagacg
     6001 ttagaaaaga agccgattcc gccgcgcaaa accttaattt tacaggtaaa tactttgtcg
     6061 atgccgatca tattaacctc aacacagtag ggccttttgt tgcatctgct gattttttca
     6121 cactggatgt cgcatcgttt atcggtaaac aaagttctga agaagaagtg aaccgatttt
     6181 tatcttcctg caaaaaatac ctggggaaat taagcatacc gggtatgagt atgccccttc
     6241 agataaccga agatttactg agaaatattg cctgtaaatt tttagctgcc actcagcagg
     6301 cagccgacat ttacactttc ctgaaaaacg aaaagggtgc aaacaatttt atcacggaag
     6361 tatcgatgga tgaagtggaa tcgccacaaa cccctgtcga aatgcttttt attcttaaga
     6421 tgctggcaga caagggtgtt cctgctcaga ccattgcgcc aaaatttacc ggacgtttca
     6481 ataaaggtgt tgattatgtg ggagatttga ctcaatttga aaaggaattc gaagaagatg
     6541 tgctggtaat tgattttgca gtaaaagaat tcgggcttcc cgaagaactg aaattgagcg
     6601 ttcactccgg cagtgataaa ttctccattt atccgataat ggcaaaaata atacgcaagt
     6661 acgataaggg attacacttg aagacggccg ggaccacgtg gctcgaagaa gtcataggcc
     6721 tggcagtggc tggaggtgaa gcattggcgc tggccaaaaa gatttatgcc ggttcataca
     6781 accggaaaga cgaactgtgt gcgccttacg ctgatgtcat tgatatagac tccactaagc
     6841 ttccttctgt ggaggaagtt aaccgatggt cgagtgaaaa attcgcgaac acattgcgcc
     6901 atatccctgg acatcccgac tacaattcca acttccgaca actcattcac gtagcttata
     6961 aagtagctgc agaattggaa aatgaataca ccgatgcact taaacaatat gcagacataa
     7021 tcggatcctg cgtggaagaa aacatttatg acaggcacct gaaaaggctt ttcaatttat
     7081 aaacgtaatt aacgtcacat aaatacttaa aatttcatgc aaaaagtcgt aacctccggg
     7141 gaagatcatg
//
LOCUS       DAOSDR010000139         7119 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrimonas sp. isolate AMR_MDS_1826
            NODE_3851_length_7119_cov_3.726217, whole genome shotgun sequence.
ACCESSION   DAOSDR010000139 DAOSDR010000000
VERSION     DAOSDR010000139.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863355
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrimonas sp. (wastewater metagenome)
  ORGANISM  Petrimonas sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Dysgonomonadaceae; Petrimonas.
REFERENCE   1  (bases 1 to 7119)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7119)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:10:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,720
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 35
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7119
                     /organism="Petrimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3851_length_7119_cov_3.726217"
                     /isolate="AMR_MDS_1826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2023866"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..239
                     /locus_tag="PLI30_06695"
     CDS             <1..239
                     /locus_tag="PLI30_06695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="acetylhydrolase"
                     /protein_id="HOI79276.1"
                     /translation="QPEARIKVVGLLPRRNKESEVTAINRQISKMASLNNYRYLDVGN
                     KLLTKSGKIDESLFLDGLHPNEKGYSLIVNDIIE"
     gene            266..1243
                     /locus_tag="PLI30_06700"
     CDS             266..1243
                     /locus_tag="PLI30_06700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019540616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HOI79277.1"
                     /translation="MEVSASFWIGFIILVVILLSLDMFVFNKKGTVIDVKKALWLSLF
                     WISLALVFNAGVYVVFGKESALEFLTAYLIEESLSIDNLFVFIIIFTAFKIKPEHQHS
                     VLFWGILGAIVFRAVFIFAGVALIERFAWIMYVFGAFLLFTGAKMVADEFRVLDHEKE
                     KEKKDPNNNGLVKIFKRVMPVTDDMSEPTFFKKIDKRLYATPFFLALLVIEFTDLIFA
                     IDSIPAVLSVSTNTFIVYTSNIFAILGLRSLYFALRGIMDLFYYLKYALSGILIFIGF
                     KMIINHYAHTVAWDFHISNVASLLVIASFLLISIAASLVRSRVVKQAVV"
     gene            1436..2314
                     /locus_tag="PLI30_06705"
     CDS             1436..2314
                     /locus_tag="PLI30_06705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006801435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HOI79278.1"
                     /translation="MSKKKKISTARFVNAKITSTISISLVLVLLGLTILILFMGNGLS
                     KFVKENMSFSVMLDSDVTDAGIQRLRKNLDGQPFVKSSRFISKDEAKEQLIKDLGEDP
                     EELLGYNPAQDAIEVYLHSEYANSDSIAVISQVIRQQNNVRDLLYQQEAIDLINDNLS
                     KVTLILLVLAAVLMFISFTLIRNTIRLSVYSKRFIINTMKLVGANGSFIRRPFILGNI
                     LTGIVSGIFADGIIFLVLAYFNKEYAELQSIIGATELIVIFVVVILLGILISTVATYF
                     AVNRYLKMKTENLYYV"
     gene            2429..2674
                     /locus_tag="PLI30_06710"
     CDS             2429..2674
                     /locus_tag="PLI30_06710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007559577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3098 domain-containing protein"
                     /protein_id="HOI79279.1"
                     /translation="MSHKNLAFHKINLIICGVAVLVIIAGFLLMSGPATTFDGFEPDI
                     FSARRIKVAPLVCLAGFVLMIVGILYPAKDRKKEEIK"
     gene            2691..3575
                     /locus_tag="PLI30_06715"
     CDS             2691..3575
                     /locus_tag="PLI30_06715"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006841856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HOI79280.1"
                     /translation="MSILEAIIIAVVEGLTEFLPVSSTGHMIITQALLGIESTPFVKA
                     FTVIIQFGAILSVVLLYWKRFFRLNPVVIFDKSAVAGKGFVQKGWTFLRRFLHKFDFY
                     WKLFIAVVPAGVLGLTFGDQIDLLLENVFVVSVMLVAGGIVMLFVDTWFNRPVENQSI
                     SWQRALKIGFFQCIAMIPGVSRSMATIVGGMSTRLDRKNAAEFSFFLAVPTMAAAAGY
                     KLYQLIKDPASAAMLQENIGVLIIGNVVAFVVAIIAIRFFIDFLTRHGFKAFGWYRII
                     VGVLLIVLLLSGTVSNVA"
     gene            3577..4278
                     /gene="truB"
                     /locus_tag="PLI30_06720"
     CDS             3577..4278
                     /gene="truB"
                     /locus_tag="PLI30_06720"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011966838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004730 - pseudouridylate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HOI79281.1"
                     /translation="MDFIEGEILHIDKPLHWTSFRLVRVVRAKLCQKLKIKKLKVGHA
                     GTLDPLATGVMTVCTGKKTKEIERLQAQTKEYIAEIRLGATTPSFDLETEIDAEYPVE
                     HISREKVEECLKQFTGKIEQIPPLFSAVKIDGKRAYEFARKNEEIELKPKILVIKEIE
                     LLFYNLPTITIRVVCSKGTYIRALARDIGESLQSGAHLLALQRTRVGDVSLNDCLKVE
                     ELDDFFDRQLAKGEA"
     gene            4327..>4655
                     /locus_tag="PLI30_06725"
     CDS             4327..>4655
                     /locus_tag="PLI30_06725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005844013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosylmethionine:tRNA
                     ribosyltransferase-isomerase"
                     /protein_id="HOI79282.1"
                     /translation="MKLSQFKFNLPEELIAKYPSRHRDESRLLVVHRNSDKIEHLVFK
                     DILNYFDAHDFFVFNDTKVFPARLFGNKEKTGAKIEVFLLRELNPSQHLWDVLVNPAR
                     KIRIGNK"
     assembly_gap    4656..4755
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4756..4986
                     /locus_tag="PLI30_06730"
     CDS             <4756..4986
                     /locus_tag="PLI30_06730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005844013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosylmethionine:tRNA
                     ribosyltransferase-isomerase"
                     /protein_id="HOI79283.1"
                     /translation="TDGHIKPREGWTNKFIFPPYDFTLPDALVTNFHLPFSTLLMVTT
                     AFGGYERIMDVYQTAIKEKYEFGAYGDAMLII"
     gene            5022..5507
                     /gene="folK"
                     /locus_tag="PLI30_06735"
     CDS             5022..5507
                     /gene="folK"
                     /locus_tag="PLI30_06735"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006801430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HOI79284.1"
                     /translation="MPDNDIHVVFLGLGSNLGDKEKNIERAYREIDKRIGRIISKSAF
                     YVSDPEGFESENRFVNSVCELVTILEAREVLRVTQAIEKELGRTKKSPDGGYADRTID
                     IDILMVDNRIVEEPGLIIPHPRFHLRDFVLTPFAEISPDTVHPVFAKTILQLKNEFGY
                     L"
     gene            5676..6656
                     /locus_tag="PLI30_06740"
     CDS             5676..6656
                     /locus_tag="PLI30_06740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI79285.1"
                     /translation="MRKSVLKNSCLLMAAILFVSLGLTSCEKIQEDFQSKHYLRCEIK
                     GEKFEKVDFLPPINFGPPIGMNARYDYMDNDSIYFTFSQFCSPVDKTSQEPEFLVRVC
                     LPTTEPIVVGKRYYVKSEGAQSKNPPLFELTSWEEVPLEQHESFNVTEIKTKESFGEG
                     YIEFVRLDLDVTKPDNGHVYAKFEYEVPSPLHENNSTMWKVKGEFQTGVSKDKNRAMH
                     DFVRGWEYTYVEGGKEYTTEYYFSYNGAFPGNKFWTTYENGEKIRVAEALSFKSDNLL
                     FDEVLVTMQTHQEYWKVMRLSSSEMEIKIKDENTGLYGETIALKSIKENK"
     gene            6678..>7119
                     /locus_tag="PLI30_06745"
     CDS             6678..>7119
                     /locus_tag="PLI30_06745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI79286.1"
                     /translation="MKTNTKSKFLIPLLVFILMPSCEKIEHESLFFYYDYREVPVNGT
                     VLIGPKTGSGNYTLEVENPLLFSAEMQTGWSSAAGMIAIQGWLKGDTRLTVTDNLTKE
                     SQKLKIKVTDNYEVMRISKANKTDNGEVPPFPASLNTIEWICLVN"
BASE COUNT         1935 a         1357 c         1672 g         2055 t
ORIGIN      
        1 gtcaaccgga ggccaggata aaagtcgtcg gattgcttcc ccgacgcaat aaggagtcag
       61 aagtgacagc catcaaccgg cagatctcca agatggcctc cctgaacaat taccgttacc
      121 tggatgttgg taacaaatta ttgacgaaaa gcggaaagat agacgagagc ctgtttctgg
      181 atggtttaca tccgaatgaa aaaggctatt cgctgatcgt taacgatata atagaatagt
      241 ttttagaatt acaagagtaa acgatatgga agtaagtgct tctttttgga taggttttat
      301 catccttgtg gtgattctcc tgtcgcttga tatgtttgtt tttaataaga aaggtacggt
      361 tatcgatgtg aaaaaagcgt tgtggctcag ccttttctgg atttcacttg ccttggtctt
      421 caacgcggga gtttacgttg tcttcggcaa ggaaagcgcc cttgagtttc ttacggctta
      481 tcttatcgag gagtcgctga gcattgataa cttatttgtt tttatcatca tttttacggc
      541 tttcaagatt aaacccgagc atcagcacag tgttttattc tggggaattt tgggagcgat
      601 tgtttttcgg gcggtcttta tttttgccgg tgtggccctg attgaacgat ttgcctggat
      661 catgtatgtg ttcggtgcat tcctgttgtt caccggtgcc aagatggtgg cagatgagtt
      721 cagggtgttg gatcacgaaa aggaaaaaga gaagaaagat ccgaacaaca acgggttggt
      781 gaaaattttc aaaagggtta tgccggtaac cgatgatatg tcggagccaa catttttcaa
      841 aaaaatcgat aaaagactat acgccacccc tttctttctg gcattgctcg ttatagagtt
      901 taccgatctt atttttgcca tagactccat tccggcagtg ctctcggttt cgaccaatac
      961 gtttattgtt tacacgtcga atatttttgc gatcctggga cttcggtcgc tctatttcgc
     1021 attgcggggc atcatggatt tgttttatta cctgaagtat gcgttgagcg gaattttgat
     1081 cttcatcggt tttaaaatga tcatcaatca ttatgcccat actgttgcgt gggattttca
     1141 tatttccaac gttgcgtcgc tgcttgttat tgcttcgttt ttactcatct caattgcggc
     1201 atcccttgtc aggagccgcg tcgtaaagca ggcggtggta tgagagcctg taactgtccg
     1261 ggaaattgta gataaaatgg gccgcgacaa cgagattttg gaaaattaat ccttcttgtt
     1321 gattattacc ggagtaaatt tccagttttc gcaatatttt tgttactttg cagccaattt
     1381 ggaaaaaacc gttttattga gaacaatcgg gtttttcttg gtgtaaccct tttttatgtc
     1441 gaaaaagaaa aagatatcca ctgcgcgatt tgtaaatgca aaaattacgt ccaccatcag
     1501 catttcgctg gtgttggtct tgttaggcct gaccatcctt attctgttta tgggcaacgg
     1561 gttgtcgaaa ttcgtgaaag agaacatgag cttcagcgtg atgcttgata gcgacgttac
     1621 cgatgccggg attcagcggt tgaggaaaaa cctggacggc cagccgtttg taaaatcttc
     1681 ccggttcatt agcaaggatg aggcaaaaga acaattgatc aaagacttgg gcgaagatcc
     1741 cgaagagttg ctggggtata atccggcaca ggacgccata gaggtttatc tccattcgga
     1801 gtatgccaac agcgacagta tcgcggtcat cagtcaggtg atccggcagc aaaacaatgt
     1861 tcgggacttg ctgtaccagc aggaagccat cgaccttatc aacgataacc tatcgaaggt
     1921 tacccttatt ttacttgttt tagcggctgt tttaatgttt atttccttta cgctgatccg
     1981 aaacaccatt cggttgagtg tttactccaa gcgatttatt atcaatacga tgaagcttgt
     2041 tggcgccaac gggagtttta tccgccggcc gtttatcctc ggtaatattc tcacgggaat
     2101 cgtttcggga atttttgcag acggcatcat ctttctggtc cttgcgtatt ttaacaaaga
     2161 atacgcagag ttgcaatcca tcatcggtgc gacggaactg atcgttattt ttgttgtcgt
     2221 catcttgctg ggaattctga tttcgaccgt ggcgacttat tttgcggtga accgctacct
     2281 gaagatgaaa accgagaact tgtattatgt ataatttaat agatttcaaa caaaagatgt
     2341 tacatacaat ccaagagtct ttattcagta tagcgatctg acatcttact caacacgcat
     2401 aaaactccaa actcataatt taaaaagtat gtctcacaaa aatcttgctt tccataaaat
     2461 aaacctcatc atttgcgggg ttgccgtgct tgttatcatc gctggttttc tgctgatgag
     2521 cggccctgca acaacttttg acggttttga acccgatatt ttcagtgcgc gacgcatcaa
     2581 agtagcccca cttgtttgtc tggccggctt tgtgctgatg attgtcggaa ttttgtatcc
     2641 ggcaaaggac aggaaaaagg aagaaataaa ataattccct gataagttag atgagtattt
     2701 tagaagcaat tattattgct gttgtagagg gcttgacaga gtttttgccc gtttcttcga
     2761 ccgggcatat gatcataact caggcgctgc ttgggattga gagtacaccg tttgtaaagg
     2821 catttacagt gattattcag tttggagcca ttctgtcggt ggtacttctt tactggaaac
     2881 ggttttttcg actgaatccg gtggtgattt tcgataagag cgccgtagca ggaaaggggt
     2941 tcgtacagaa agggtggaca tttctaaggc ggtttctgca taagttcgat ttctattgga
     3001 agctgtttat cgctgttgtc ccggcaggag tgttggggtt gacttttgga gaccagatcg
     3061 acttgctgct cgagaatgtc tttgtcgtga gcgtgatgct tgtcgcaggc ggtatcgtga
     3121 tgctttttgt ggatacgtgg ttcaatagac ccgttgagaa tcaatcgata tcgtggcagc
     3181 gggcgttgaa gatcggtttc tttcagtgta ttgcgatgat tccgggtgtt tcccggtcga
     3241 tggctaccat tgtgggaggt atgagcaccc ggctcgaccg gaaaaacgcc gctgaatttt
     3301 cgtttttcct ggctgtacct accatggctg cagcagccgg ttacaagctg tatcagctga
     3361 taaaggatcc tgccagtgca gcaatgttgc aggaaaatat aggagtgttg attatcggaa
     3421 acgtggttgc ttttgtggtt gccatcattg ctattcggtt ttttatcgat tttctgaccc
     3481 gacacggatt taaggcattt ggatggtaca gaattatcgt aggtgttctg ctgatcgtgt
     3541 tgctgttatc cggaaccgta tcaaacgtag cgtgatatgg attttattga aggagagatc
     3601 ttacacatcg ataaaccgct gcattggact tcgttccggc tggtgagggt agtacgggcg
     3661 aagttgtgcc agaaactgaa aattaaaaag ctgaaagtag ggcatgcggg aacactcgat
     3721 ccgctggcta caggtgtaat gaccgtttgc acgggaaaaa agacaaaaga gattgaaagg
     3781 ttacaggctc aaacaaaaga gtatattgcc gagatccggc tgggagcaac tactccctct
     3841 tttgacctgg agacggaaat tgatgccgaa tatccggtgg aacacatctc acgcgaaaag
     3901 gtggaagagt gcctgaagca attcaccgga aaaattgaac agatcccgcc tcttttttcg
     3961 gcagtcaaga tcgacggtaa aagagcgtac gagtttgccc ggaagaatga agaaattgaa
     4021 ctaaaaccaa agatattggt tataaaggaa attgaattgt tgttttacaa tttaccaacg
     4081 ataactatcc gggttgtttg cagtaaaggc acgtatatac gtgctttggc gcgcgacata
     4141 ggcgaaagcc ttcagtcggg tgcacatctt cttgctctcc aacgaacgag ggtgggagat
     4201 gtatcattga atgattgttt aaaggttgaa gagctggacg atttttttga ccggcagcta
     4261 gccaaagggg aggcttaaca ataaattatt tcacgatagt attataaaga atcagtttaa
     4321 tcacatatga aactttctca gtttaaattt aatttaccgg aggaattgat tgccaagtat
     4381 ccgagtagac atcgcgatga atccagattg cttgtggttc atcgaaattc cgataaaata
     4441 gaacatcttg tttttaagga tattctgaat tactttgatg ctcacgactt tttcgttttc
     4501 aacgatacca aggtctttcc tgcacggttg ttcggaaaca aagaaaaaac cggagccaaa
     4561 atagaagtgt ttttgctcag ggagttgaat ccctcgcagc atctatggga tgttttggtt
     4621 aatcccgccc ggaaaataag gatcgggaac aagttnnnnn nnnnnnnnnn nnnnnnnnnn
     4681 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4741 nnnnnnnnnn nnnnnacaga cgggcacatc aagccgaggg agggatggac gaacaagttt
     4801 attttcccgc cttatgactt tacactaccc gatgcgttgg ttaccaactt ccacctgccg
     4861 ttctccacgc ttttgatggt gacgactgct ttcggcggat acgaaagaat tatggatgtg
     4921 tatcagacgg ccatcaagga gaaatacgag tttggtgcgt acggggatgc tatgctgata
     4981 atttaatacg cgcactttgt gcgatgatgc tacgctgaaa aatgcctgat aacgatattc
     5041 atgttgtttt tttgggattg ggctccaacc tgggagacaa ggaaaaaaat atcgagcgtg
     5101 cctacagaga aattgacaag cggataggga gaattatttc caaatccgct ttttatgtgt
     5161 ccgatccgga gggtttcgag tctgaaaaca ggttcgttaa ttccgtttgt gaactggtca
     5221 caatcctgga agccagagag gtactgcggg taactcaggc gattgaaaag gaactggggc
     5281 ggacaaaaaa atctccggat ggtgggtatg ccgatcgcac catcgatatt gacatcctga
     5341 tggttgataa ccgaatcgtt gaggaacccg gattaatcat tccgcatccc cgcttccatt
     5401 tgcgcgactt tgtgttgacc ccttttgccg aaatctcgcc agacaccgtt catccggtgt
     5461 ttgccaaaac cattttgcaa ttgaagaatg agtttggcta cctttaatcg tgcatactgc
     5521 cttccgaaac cataccattc gaggcggcag ctatttttcc tttcctgtca tttatttctt
     5581 tttttttata tttaacatta tttgtgcagc tttttctcag attctacgtc ttacgtaaaa
     5641 gaacagtcaa ttagaatctt tagacaatca ttacaatgag aaaatctgta ttgaaaaaca
     5701 gctgtctgct tatggcagcc atcctttttg tgtcacttgg gttgacaagt tgtgagaaga
     5761 tacaagaaga tttccaatcc aaacattact tacgttgtga aataaagggt gagaagtttg
     5821 aaaaggtaga ttttttacct ccaataaact ttgggcctcc cataggaatg aatgcccgct
     5881 atgattatat ggataacgac agtatttact ttaccttctc gcagttttgc agtcctgttg
     5941 acaaaacgtc tcaagaacct gaatttttgg taagggtgtg tctcccaaca accgaaccca
     6001 tcgttgtggg taaaagatat tacgtcaaat ccgaaggtgc ccagagtaag aatccgcctt
     6061 tgtttgagtt gacatcgtgg gaggaagttc ctcttgaaca acacgaatcg tttaatgtga
     6121 cagaaattaa aacaaaggaa tcctttggag aggggtacat cgaatttgtg cgtttggatt
     6181 tagacgtaac aaaacccgat aacgggcatg tatatgcaaa atttgagtat gaggtaccct
     6241 cacccctgca cgaaaataat tcaacaatgt ggaaagtgaa aggcgagttt caaaccggtg
     6301 tcagcaagga taaaaacagg gctatgcatg actttgtcag aggctgggaa tacacttacg
     6361 tggaaggcgg taaagaatac acaacggaat attatttttc gtataatggt gcgttcccgg
     6421 gaaacaaatt ctggaccact tatgagaatg gggagaagat aagggtcgca gaggcacttt
     6481 ccttcaagtc cgataactta ttgtttgatg aagtgttggt taccatgcaa actcaccagg
     6541 aatactggaa ggttatgcgt ttatcttctt ctgaaatgga gattaaaatt aaagatgaaa
     6601 atacgggttt gtatggcgaa acaattgcgc taaaatcaat caaggaaaat aaataatcac
     6661 ataaattttg tacaaatatg aaaaccaaca caaaaagcaa gttcctgata ccgcttttgg
     6721 tattcatttt aatgccaagt tgtgaaaaaa tagaacacga atcactcttt ttttattacg
     6781 attatcgcga agtgcccgtg aacggaacgg tcttaatcgg gccgaaaaca ggcagcggaa
     6841 attacacgct ggaagtagaa aacccgcttc tgttttcggc agaaatgcaa accggctggt
     6901 cgagtgcggc gggaatgatt gccattcagg ggtggctcaa aggagatacc cggctcacgg
     6961 ttacggataa cctaaccaaa gaatcgcaaa aattgaagat taaggtgacc gataactatg
     7021 aggtgatgcg catatccaaa gctaacaaaa cagataacgg cgaagtgcct cctttccccg
     7081 cttccttgaa cactatagaa tggatttgcc tggtaaaca
//
LOCUS       DAOSDR010000140         7093 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrimonas sp. isolate AMR_MDS_1826
            NODE_3868_length_7093_cov_3.566354, whole genome shotgun sequence.
ACCESSION   DAOSDR010000140 DAOSDR010000000
VERSION     DAOSDR010000140.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863355
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrimonas sp. (wastewater metagenome)
  ORGANISM  Petrimonas sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Dysgonomonadaceae; Petrimonas.
REFERENCE   1  (bases 1 to 7093)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7093)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:10:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,720
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 35
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7093
                     /organism="Petrimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3868_length_7093_cov_3.566354"
                     /isolate="AMR_MDS_1826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2023866"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..275)
                     /locus_tag="PLI30_06750"
     CDS             complement(<1..275)
                     /locus_tag="PLI30_06750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005795638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HOI79287.1"
                     /translation="MKFVGAHISASGGVENAPVNAHLIGAKAFAFFTKNQRQWFAAPY
                     TQQNIDLFKSRCEEYGFSPDHILPHDSYLINLGHPEEEGLKKSRSAF"
     gene            495..815
                     /locus_tag="PLI30_06755"
     CDS             495..815
                     /locus_tag="PLI30_06755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005860908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HOI79288.1"
                     /translation="MLITTTNTIEGKHIVAYYGIVSGETIIGANIVRDFFASITDVIG
                     GRSGGYEKVLREAKDIALQEMSEQARRMGANAILAVDIDYETIGNNGSMLMVSASGTA
                     VKVE"
     gene            983..1699
                     /locus_tag="PLI30_06760"
     CDS             983..1699
                     /locus_tag="PLI30_06760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HOI79289.1"
                     /translation="MKTTTKLFFTTIITLIFISSANAQDWANLTRFKEDNAKIGMPRT
                     CDDRVVFMGNSITQGWIEKVPKFFENRPYINRGISGQTTPQMLVRFRQDVINLYPKVV
                     VILAGTNDIAGNTGPSTLEMIEDNIHSMTELAQSNGIQVVLCSVLPAFDYKWKPGLEP
                     AGKIVELNRRIKKYAETHGAVYCDYFSAMADERNGLPESLSGDGVHPNPEGYALMAPI
                     AETAIARALLMWKDSPKRKY"
     gene            1799..2932
                     /gene="pdxB"
                     /locus_tag="PLI30_06765"
     CDS             1799..2932
                     /gene="pdxB"
                     /locus_tag="PLI30_06765"
                     /EC_number="1.1.1.290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005864030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033711 - 4-phosphoerythronate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-phosphoerythronate dehydrogenase PdxB"
                     /protein_id="HOI79290.1"
                     /translation="MKILADAHIPYLKDIAEQFGEVEYLPGNQFTKEAISDKHALIVR
                     TVTHFGKDILEGTGVKLICSATIGFDHIDTRYCDAKGITWRTAPGCNANSVEQYVTAS
                     LLYLADKYGFSLKEKSIGIIGVGNVGSKVETACRKLGMRVLLNDPPREEREVGQKSGG
                     MEKSVFVDLETVKKEADIITLHTPLTKSGKHKTYHLVDEYFFDTLDKKPFVINSCRGS
                     VVDNTAMKKALKTGKITGAVIDCWENEPDIDRELLQMADISTPHIAGYSADGKWTATK
                     MSLENLNEFFELDVYPIKLMQLPQPNNPVIDLREIEPDHQLAYAVWQTYNPMMETMNL
                     KADPDKFYWFRSNYPLRREYGAYEVKNADPGVVPILKQLGFDV"
     gene            3139..4830
                     /locus_tag="PLI30_06770"
     CDS             3139..4830
                     /locus_tag="PLI30_06770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008661623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI79291.1"
                     /translation="MRKISFYALLAGLVFAAASCSNLKPLSQSNFNATPSPLETVGNS
                     VPVTVNGTFPEKWFNKNATVKITPVLKYAGTKESYGSAQAYQGENVSGNAIEIPYNRG
                     GNFNMSFTFPYQPDMLTSELFMRFDAKIKNKSVKLPEVKVADGIVATSALASAQTTAP
                     SVADDGFQRIIKQTQDANIHFVIQQANIRSSETNTQDMRSWQQRVQDAFNDPRQNVDI
                     EISAYASPDGGLTLNERLAAQREKNTTQYLEGELKRRKIDTDVNARYTAQDWEGFRQL
                     LEASDLQDKELVLRVLSMYPDPETREREIKNISFVYSDLASTILPQLRRSRITANIEI
                     IGKSDEEIMEFWRANPKKLSVEELLYASTLTDNDADKEKIYQYVTVNFPQDYRGWNNM
                     ATAYYQRGEYNNAKQALDRAALVSPNAAEVNVNKALFAMMDGNTQSAKELLGRSSGAN
                     NLEAAMGLLYLMEGDYNQAVDAFGDTKSNNAALAQILTKNYNAADITLKAIKNPDALT
                     YYLMAVVGSRTNNFNDVMTNLRSAITMDRTMAARALDDLEFAKYRTNQDFMTLLR"
     gene            4988..5767
                     /locus_tag="PLI30_06775"
     CDS             4988..5767
                     /locus_tag="PLI30_06775"
                     /inference="COORDINATES: protein motif:HMM:NF024140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MCP four helix bundle domain-containing protein"
                     /protein_id="HOI79292.1"
                     /translation="MLKSLRAKIFSSFMLLVLMLVIAGIMSIIEFNKVGVSIKNVMDD
                     NYKSIEQTKQMLDALEREDSGLLMYLMGNREMGSQTINTAYSAIQDAIKIAQNNITEK
                     DEDKYINNIIKKYEEFHTSVKQTTDTSEQLTLEEKNEIYLQNQQLFFSAKKAINELMQ
                     LNQEGVYKQTNTMKENSKRAMMPAIVSIVAAIVFALLLNFFISEYFIRPINRLIDGVK
                     SFYPEKGIINSGIKSNDEIKRLETETNNLIGRLLRLKNQSK"
     gene            5764..>7093
                     /locus_tag="PLI30_06780"
     CDS             5764..>7093
                     /locus_tag="PLI30_06780"
                     /inference="COORDINATES: protein motif:HMM:NF014443.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HOI79293.1"
                     /translation="MRVSVIAKYLGFVLLFNALFMYISAVISIFLRETSITPLLYSAI
                     LCTIVGVFPQIFVEKTEELKPHEGIAISVLGWILTCIVGAIPYFMWGGEFTLTNAIFE
                     STAGYTTTGSTILTNIEMLPKGLLFWRSATHFIGGMGIILFVLLILPNAKAARASIYR
                     SEVSGLSMLNFQMRMREIVRIIGIVYASLILAETIILWALGMTFFDAICHSFGTLATG
                     GFSTKNTSIAFYNNIWIEITISFFMLLGGMHFGLIYATITGRKQNLFTSDVVKTYLAI
                     IFIGILFISFKLVNDHVYNWGEAFRHASFQVVSLVTTTGFATVDTSVWPMFTIVVLIY
                     FSIQCAMIGSTTGGLKFDRVYLFFQTFLKQIKQTNHPNAVYVLKMDKTLISSDIELQS
                     MVYIIVYLVILLISTLALSAMDIDLITSFSGSVATLGTIGPGFGKTIGSMG"
BASE COUNT         2270 a         1431 c         1445 g         1947 t
ORIGIN      
        1 aaaaaggcgg aacgcgattt tttcaagcct tcttcttcag gatgccccag gttgatcagg
       61 taactgtcgt gcggaagaat atgatccgga gagaaaccgt attcttcaca cctcgattta
      121 aacaaatcaa tattctgctg cgtataaggt gcggcaaacc attggcgctg attcttggta
      181 aaaaacgcga aagcctttgc tcctatcaaa tgcgcgttaa ccggtgcatt ttccactccg
      241 cccgatgcgc ttatatgtgc tcctacaaat ttcatcttat ctgttttttt aatttttaca
      301 tttaaagtcc gttatctgtt tttacagtag acaaccattg ccaacgaacc ttgtcggctg
      361 gtagccgcaa agatacgtga taaagattga aagaataaaa aatgcattgt ttttctgcgg
      421 aatttcttac caaaagaata aaatttatta tctttcgaaa aaaataacaa ttgaataaaa
      481 ccattctttg ctctatgttg attacaacaa caaacactat tgaaggaaag cacattgttg
      541 catattatgg gatcgttagc ggagaaacca ttatcggtgc caatattgta agggattttt
      601 ttgcttccat tacggatgta attggaggac gttccggagg ttacgagaaa gttttgcgcg
      661 aagcgaaaga tattgcgttg caggaaatga gtgaacaagc ccggagaatg ggtgcaaatg
      721 ccattctggc ggtcgacatc gactacgaaa ccatcggcaa caacggcagc atgctgatgg
      781 tttcagcatc gggaacagca gtgaaggtag agtaacaaga cccgtcattt agaaaccaac
      841 tgtcagttgc acacacttca aaaataaagc ggaggcctct gcccgttact ggttccgggg
      901 taaatattct cccgattttt gtttgaaaac ttttcacaaa caaaacagca gttttgacga
      961 caaacacaat ataattagac aaatgaaaac gacaacaaaa ttatttttta caaccattat
     1021 tactttgatt ttcatatctt ctgctaatgc gcaggattgg gcaaatttaa cgcgtttcaa
     1081 agaagacaat gcgaaaatag ggatgccaag gacgtgtgac gaccgtgtgg tttttatggg
     1141 caactccatc acacagggat ggatagagaa agtaccgaaa ttctttgaaa acagacctta
     1201 cattaaccgg ggtataagcg gacaaaccac acctcaaatg cttgtccgct tccgccagga
     1261 tgtgataaac ctttatccga aagtagtggt tattcttgcg ggaacaaacg atattgccgg
     1321 aaacaccggg ccttcaacac tagagatgat cgaagacaac atccattcta tgactgaact
     1381 tgcacagtcc aacggaattc aagtagtgct ttgctctgtt cttcctgcat ttgattataa
     1441 atggaaaccc ggccttgaac ccgcaggaaa aattgtagaa ctgaaccgta ggataaaaaa
     1501 atatgcagaa acacacggtg cggtttattg cgactacttt tcagctatgg ccgatgagcg
     1561 aaacggatta cctgaaagct tatcaggcga tggtgtacat cctaacccag aaggatatgc
     1621 ccttatggct cccattgccg agacagctat tgcccgggct ttgttgatgt ggaaagatag
     1681 tccgaaaagg aaatactgat ttaacttaca acctacgaat atcaagttga aaacccggac
     1741 tttgagtctt tcagaagaga gatttattcc tacgttttga ctctttgttt atataaatat
     1801 gaaaattctt gcagacgcgc atattcctta tctaaaagac attgccgaac agtttggcga
     1861 agtggaatac ttgcctggaa accaattcac aaaagaagct attagcgata aacacgcact
     1921 tattgtacgt acggttactc attttggaaa agatattctt gagggaaccg gtgtaaaact
     1981 aatttgctcc gctacgatcg gcttcgacca tatcgacacc cgatattgtg atgcaaaagg
     2041 cattacctgg cgcacagccc cgggttgtaa cgccaactca gtggaacaat acgtaacagc
     2101 ctcgctcttg tatttggcag ataaatacgg attcagttta aaagaaaaat caataggaat
     2161 tattggtgtt ggcaatgtgg gtagtaaagt ggagacggct tgccgaaaac tgggaatgcg
     2221 tgtattacta aatgacccac cgcgggaaga gagggaagtc ggacaaaaat ccggaggaat
     2281 ggaaaaatct gtttttgtgg atctggaaac ggtaaaaaaa gaagcagata tcatcaccct
     2341 gcatacacca ttgacaaagt ccggaaaaca caaaacctat catctggtgg acgaatattt
     2401 ctttgacaca ctggataaaa agccgtttgt catcaattca tgccgcggaa gtgtggtgga
     2461 taacacagcc atgaaaaaag cgttgaaaac aggaaaaatt actggagccg taattgattg
     2521 ctgggaaaat gaacccgata tcgaccggga actattacag atggccgaca tctccacacc
     2581 acatatcgcc ggatattcgg ctgacggcaa atggacagca accaaaatgt cgcttgaaaa
     2641 cctgaacgag ttttttgaac tagatgtcta tccaataaaa ttaatgcagc ttcctcagcc
     2701 gaataatccg gttattgact taagggagat tgaaccggat catcaattgg cttatgcagt
     2761 ctggcaaacc tataacccca tgatggaaac aatgaatctg aaagcagatc ccgataaatt
     2821 ctactggttt cgatccaact atccgctgcg tagggaatac ggagcttacg aagtaaaaaa
     2881 tgcagaccca ggggttgttc ctatcttaaa acaactcggg tttgatgtat aatcaacccg
     2941 gcaattttca cgtcgatgga tatttctaaa aaatacctaa atatgatcgg tttatttttt
     3001 gatatcacat aaaaaaggta attttgttgt atgttgtttc aaattatcca ggatagagag
     3061 caagtgagta taatgatgga taatgcgatt atctcttttg gaaaattcaa taaacaaaaa
     3121 ataaaaatca atcggaaaat gagaaaaatt tctttttatg ctctgctcgc cgggcttgtt
     3181 tttgctgctg catcgtgcag caatttaaag cctctttcac aatcaaactt caatgcaacc
     3241 ccatctccct tagaaactgt tggcaacagt gtgcccgtaa ccgttaacgg aactttcccc
     3301 gagaaatggt tcaacaaaaa cgcaaccgta aaaattactc cagtattgaa atatgccggt
     3361 accaaagaat cctacggatc tgctcaggct taccagggag aaaacgtttc agggaacgcc
     3421 attgaaattc cgtataaccg tgggggaaac ttcaacatga gtttcacctt tccctatcaa
     3481 cccgacatgc tgacttcgga actttttatg cgttttgatg ccaaaatcaa aaataaatcc
     3541 gtaaaacttc ccgaggtaaa agtagccgat ggcatcgttg ccacttcggc actggcaagt
     3601 gctcagacaa ccgctccatc tgttgctgat gacggttttc aacgcatcat aaagcagact
     3661 caagacgcta acattcattt cgttatccaa caggcaaata ttcgttcatc cgaaacaaac
     3721 acgcaagata tgcgttcgtg gcaacaacgg gttcaggatg cattcaacga tccgcgccag
     3781 aacgtagaca ttgagatttc tgcttacgca tcgcccgacg gaggtcttac gttgaacgaa
     3841 agattagctg ctcaacgtga gaagaacacg acacaatacc tggaaggtga gctgaaaaga
     3901 agaaagattg ataccgatgt aaacgcccgc tacacggcac aagactggga agggttccgc
     3961 caattgctgg aagcatcgga tctacaagat aaagagctcg tattaagagt tttatccatg
     4021 tatcccgatc ccgaaacacg cgagcgtgaa atcaaaaaca tctcgtttgt ttacagtgat
     4081 ctggcatcaa ccatcttacc gcagttacgt cgttcacgta tcacggcaaa catcgagatt
     4141 atcggaaaat cagatgaaga gatcatggaa ttctggcgtg ccaatcctaa aaaactgtcg
     4201 gtggaagaat tgctttacgc ttccacgctc accgataacg atgccgataa agaaaaaatt
     4261 tatcaatacg ttaccgtaaa tttcccgcaa gactaccgag gatggaataa catggctacc
     4321 gcttattacc agcgcggcga atacaataac gccaaacagg cgttagaccg tgcagccctg
     4381 gtttcaccca acgcagccga agtgaatgta aacaaagcgc tattcgctat gatggacgga
     4441 aatacacaat cggcaaaaga attactgggc aggtcatcag gggcaaacaa cctggaggca
     4501 gctatgggat tactttacct tatggaaggt gattataatc aagctgttga tgcttttgga
     4561 gatacaaaat cgaacaacgc cgcattagcg caaatcctta caaaaaacta caacgctgct
     4621 gatatcacgc tgaaagctat taaaaatcct gatgcattga cctactacct gatggctgtc
     4681 gttggatcga gaacgaacaa tttcaacgat gtgatgacca accttcgttc cgcaataacc
     4741 atggacagaa caatggctgc cagagcatta gatgacctgg agtttgcaaa atacaggaca
     4801 aatcaggatt tcatgacttt actaagataa aatttaaaac agtttcttaa atataacgat
     4861 aaaatggagt gccggaagcg ctccattttt attttattgt aaaaaataaa ttcatatttg
     4921 taaagaaacc gtaaaacccg tacatttgtt acgaaaattt tattattcat gttaaaagag
     4981 agttattatg cttaaatcgt tgagagctaa aatattttcc agctttatgt tgctcgtgct
     5041 gatgctggta attgctggaa ttatgtcaat tattgagttc aacaaagtgg gagtatccat
     5101 taagaatgtt atggacgata actacaaatc cattgagcag accaaacaaa tgttagacgc
     5161 gttagaaagg gaagacagcg gcttgttgat gtatctgatg ggaaaccggg aaatgggaag
     5221 ccaaactatt aatacggcct attcagcaat acaggatgcc attaaaatag cccaaaataa
     5281 cattaccgaa aaagacgaag ataaatacat caataatatt ataaaaaaat acgaagaatt
     5341 tcatacttcc gttaagcaaa ccaccgatac cagcgaacaa ttaacattgg aagaaaaaaa
     5401 cgaaatatac ctccaaaacc agcaattgtt cttttcagca aagaaagcca tcaatgagtt
     5461 gatgcaactt aaccaggaag gggtttacaa acaaacaaac acgatgaaag aaaactcgaa
     5521 aagggcgatg atgccagcca ttgtatctat tgtcgctgcc atcgttttcg ctttattact
     5581 taatttcttc atatccgaat attttattcg tccaatcaat cggcttatcg atggagtaaa
     5641 atcattttac ccggaaaaag gcataataaa tagtggcatc aagtcaaacg acgaaattaa
     5701 acgcttagaa accgaaacga ataaccttat cggaagattg ctgcgtttaa aaaatcaatc
     5761 gaaatgagag tcagcgtcat tgcaaaatat ttaggttttg tgttgttatt caatgcatta
     5821 tttatgtata tctctgccgt tatttccatt tttttaagag aaacttctat aaccccctta
     5881 ctgtatagcg ctatcttatg tacaattgta ggagtgtttc cacaaatctt tgtagaaaaa
     5941 acggaggaac taaagcctca cgaaggaatt gctattagcg ttctaggatg gatattaaca
     6001 tgtatcgtag gcgcaattcc atattttatg tggggcggtg aattcacctt aacaaatgcc
     6061 attttcgaaa gtactgccgg ttatacaact accggatcca ctattcttac caacattgaa
     6121 atgttaccca aaggtctttt gttctggcga tcggcgacac attttattgg aggtatggga
     6181 attattttgt ttgtgttatt gattctcccc aatgccaaag cagcgcgggc tagcatatac
     6241 aggtctgaag tatctgggct ctccatgtta aattttcaga tgcgaatgcg ggaaattgta
     6301 cgtattattg gcatagtcta tgcgagtctg atacttgctg aaacaattat tctatgggca
     6361 ctgggaatga cctttttcga tgccatttgc cattcattcg gaacactggc aactggcggt
     6421 ttctctacta agaatactag tatcgccttt tacaacaata tttggataga aatcaccatt
     6481 tcctttttta tgttgttggg aggtatgcat tttggactta tatacgctac cataacaggt
     6541 aggaaacaaa atctttttac ttctgacgtt gttaaaactt atttagccat tatttttata
     6601 ggcatcttgt ttatttcatt caaactcgtc aatgatcacg tatataactg gggagaggcg
     6661 tttcggcacg catcatttca ggtagtttcg ttggttacta ctaccggttt tgctaccgtg
     6721 gatacgtcag tttggcccat gtttaccatt gttgtcctta tctatttctc cattcagtgc
     6781 gcaatgattg gttcaacgac aggtgggtta aaattcgatc gggtatacct cttttttcaa
     6841 acatttctta aacaaattaa acagacaaat caccctaacg ccgtttatgt gttgaaaatg
     6901 gataagaccc ttatcagcag cgatattgaa ctacaatcaa tggtttacat catcgtttat
     6961 ttggtcattc ttctaatttc tactctggca ttatcagcca tggatatcga tcttattaca
     7021 tccttttcag ggtctgttgc cactttaggc actattggcc cgggatttgg gaaaacaatc
     7081 ggatcgatgg gaa
//
LOCUS       DAOSDR010000141         7092 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrimonas sp. isolate AMR_MDS_1826
            NODE_3870_length_7092_cov_3.837999, whole genome shotgun sequence.
ACCESSION   DAOSDR010000141 DAOSDR010000000
VERSION     DAOSDR010000141.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863355
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrimonas sp. (wastewater metagenome)
  ORGANISM  Petrimonas sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Dysgonomonadaceae; Petrimonas.
REFERENCE   1  (bases 1 to 7092)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7092)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:10:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,720
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 35
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7092
                     /organism="Petrimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3870_length_7092_cov_3.837999"
                     /isolate="AMR_MDS_1826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2023866"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..613
                     /locus_tag="PLI30_06785"
     CDS             <1..613
                     /locus_tag="PLI30_06785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019539456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI79294.1"
                     /translation="SAKYLDDALIYVSAIEYNNGNLNQALADYSRLANSARNQSSQQT
                     GQMGVVRTQYRLKSFHEAARAATAFLANANLSPEVTAEARYLRGKSLQQVNEVEKAMD
                     DFQSLAQDTRNIYGAEAQFILADTYYRWKSYDKAESQVKSFMQKGTPHQYWMARALIV
                     LSDTYRAKGDNFQARQYLESLKNNYKGSEADVQQMINERLSNL"
     gene            634..2328
                     /locus_tag="PLI30_06790"
     CDS             634..2328
                     /locus_tag="PLI30_06790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI79295.1"
                     /translation="MKKTIFSVALLLVSTGIFAQQQDSLLRRQMELQREFNPTLQDAN
                     KINSLPSVPQPAIKKANTGYSPWAGRTTPPLEIAIPDPANIMTEVPFSSKKGYVSLGA
                     GNYANIDGALGYRLVENEKSNLTFNFLHSSSNGDINYVQESDPAGNKAFFMDNLGQLR
                     YGHLLSAFKLNLKAAYSHNAFNYYGNTFGNDRFYDNVNQTLGVFNLGLGVESNKSDLV
                     NYRGFFDFKNFASKYGSDLTSAGLKGNHIDAVVGLDKPFQGGDSRVGVDGKFLGVFYS
                     QGMDDFTLLGANPYVAFEGLNWKARLGADLLFQMLGGTKFRVAPNIELDLKVAGHSSL
                     YAHIKGGIDGNTYLDMMNESRYITPMTPVKSSFSIVDIEAGAKIGEVSGFRFDIFGGF
                     KKTEDEHFLVLNQIVQAETPDMPSIKESLAPLYGNFSHSHIGGMLQSNIWSPLDIALR
                     IKKNFYTVNDTGIPDAKAYNKPGFETDISTTFSATSNLKFLLNYYFAGDRWSYFNDTN
                     VQINNINDLNFGALYDINESFAIHLKANNLLLQKYDIWYGYPAQGFNAMGGFTLKF"
     gene            2386..5964
                     /locus_tag="PLI30_06795"
     CDS             2386..5964
                     /locus_tag="PLI30_06795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009134235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HOI79296.1"
                     /translation="MKNSTKVVKKGWLLILLLTILQQPVAGQLQKGKGYPKKAYESDL
                     LQHFAAPPGGYGNVPFYWWNGDSLRKERLQEQLDILSSSAVDGFAVSYIHTSPRIDTL
                     ENRDGYGLYGMTEPGVPHVFSDKWWEVWNWFSGEAGRRGLGAGLDDYTVGWNGNGYYP
                     DELDTVAVFRDYSGELVIRVDSIRGGETYNALQPENLLTVVVWPGKIVLTDSVTDGRI
                     RWRAPGKGISRIYTITTAPGYVIHPEHGNKLLDVYFNRFEERMDDAGRAGMNYFFQDE
                     LAYPIHMLTWSDDFSEEFIRRKGYDIVPYLPALKESIGPVTPRVRMDYCEVLMDLSEE
                     RYYKPIYQWHADRGLMYGCDNLSRGKDPMAYIDYFRAMSWFTAPGNDAPARGSSFLET
                     KISSSIAHLYSRPRTWLEAFHSMGWGSSGAWLTDQIDHHFVAGGNLVCMHGLYYSTHG
                     GWWEWAPPDFHFRMPYWPHMKRWLDYTERMSFILSQGSHVCDIALVYPTETIQAYPGT
                     KPDGVFDLALRLSNSGLDFDFVDFRSLRDASIEERELRMADERYKVLVLADMEAMHHS
                     SLTRALAFYRAGGIVLATGRLPRATSAKGEKDPEVEAILRELFGLTAAEVAAGKPAKK
                     QVNAAGGVGWYIPEAPERQVAGLITPDFRPGQGAGKVLHRRVGNKDIYMVKDVEKGSE
                     CFFRATGRVELWDAHSGTAQASPVLSQDKAGTRIRMNRETGNSYLIVFSPGEPLMADE
                     GRTALRLSYEIPLGREWVTELLPTLQNKWGDYRFPAFDGMIGAEARSFRYAPDNGMPG
                     NWMQPDYNDANWEEGIYGYGPQLMTQADGIAWTPVSFSWQYGVWDNPGAQGYHGLKGK
                     VDDRFFILDKGKNQRFKTYVYAPKGGVYRIESDGVLPQYVRVDGQEMKETVHLKKGWH
                     ALEVQYDRSEQLDYKPRTGLYNDTRLRGMVLFFPEKTPLPEKPSPYADEVSSRWVTGK
                     HLLFDPYGGTRNRWSFRFDAVPGLEEMTLVLQGELTDLWIDGEKAARSQILRTPGKRG
                     MNRYRVTLRKKKERTATVAFTVRAEKGYQGTAVVREPVTLKTSTGLMKSGDWSQEGAL
                     RYYSGGIRYRQSYELNNTAGAKQIMLKLGEVVATCEVTVNGQSAGVLISPPYELDITG
                     LVKDGKNDIEVLVYSTLSNHYQTIPTPYRGEPRAGLIGPVLMSVYE"
     gene            complement(6416..6985)
                     /locus_tag="PLI30_06800"
     CDS             complement(6416..6985)
                     /locus_tag="PLI30_06800"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008822752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HOI79297.1"
                     /translation="MTEKEKMIRGELYMGSGAELSGEHLNSVRLCREYNAIDPVEKGK
                     RSEILKELCPVQGDNVHIEGPFFCDYGYNIHFGNNVYINYNCTILDVTTVTIGNDVLI
                     GPSVEIYTAAHPVQWKQRATRIEFGKPVFIGNNVWIGGNTTICPGVSIGNRTIIGAGS
                     VVTKDIPDDVIAAGNPARIIKRLDTEYSL"
BASE COUNT         1837 a         1598 c         1871 g         1786 t
ORIGIN      
        1 cagtgccaag tatctcgacg atgcgctgat ctatgtgtcc gccatcgaat acaacaacgg
       61 aaacctcaac caggcactgg cagattattc cagactggct aactcggccc ggaatcagag
      121 cagtcagcaa acgggacaga tgggtgttgt ccgtacacag taccggttga aaagttttca
      181 cgaagccgct cgtgcagcaa ctgctttcct ggccaatgcc aatctgtcgc ctgaagtaac
      241 tgcagaagcc cggtacctgc gtggaaaatc actacagcaa gtcaacgagg tggaaaaggc
      301 gatggacgat ttccaatcgc tggcacaaga cacacgaaac atttacggtg cggaagcgca
      361 atttatactt gccgacacgt attaccggtg gaagtcgtac gacaaggcag agtcacaggt
      421 gaaatcgttt atgcaaaaag gaacgccgca ccagtactgg atggcgcgtg cactgattgt
      481 cctgtcggat acatacagag ccaaaggcga taattttcag gccagacaat accttgaaag
      541 cctgaaaaac aactacaaag gctccgaagc cgatgttcag caaatgataa acgaaagact
      601 aagtaattta taattcacaa tgaataattg aaaatgaaaa agacaatatt tagtgtagcc
      661 ctgttgcttg tatctaccgg tatttttgcc caacaacaag actctttgtt acggcgtcag
      721 atggaacttc aacgtgagtt caacccaacg ctgcaggatg ccaataaaat aaattcattg
      781 ccgtccgtcc cgcagccggc aatcaagaaa gccaataccg gttattcgcc ttgggcgggt
      841 agaaccacgc caccgttgga aattgctatc ccggatcccg ccaacataat gacagaggtt
      901 cctttcagtt cgaaaaaagg atatgtttcg ctcggagccg gaaattatgc caatatcgat
      961 ggggcattgg gctatcgcct tgtcgagaat gaaaagagca acctcacgtt taattttctg
     1021 catagctcat ccaacgggga tataaattac gtacaggaga gtgatccggc gggtaacaag
     1081 gcttttttta tggataatct ggggcagctc cggtacgggc atctgctcag tgccttcaaa
     1141 cttaatctca aggccgcgta ctcgcataat gcattcaatt actacggcaa tactttcggg
     1201 aatgaccgct tttacgacaa tgtaaaccaa acactgggcg ttttcaactt gggattgggg
     1261 gtggagtcca acaaatcgga ccttgttaat tatcgtggat ttttcgattt caagaatttt
     1321 gctagcaaat acggttcgga tttgacgagt gccggtttga aaggaaatca cattgacgcc
     1381 gtagttggac tggataagcc ttttcagggt ggggacagcc gggtaggggt tgatggaaag
     1441 tttcttggcg ttttttatag tcagggtatg gatgatttta cgctgttggg tgccaatccc
     1501 tatgtcgcat ttgaaggatt gaactggaaa gccaggttgg gagctgattt attgtttcag
     1561 atgctgggtg gaacaaaatt ccgggttgca ccaaacattg aactggacct gaaggttgcc
     1621 ggacattctt ccctttatgc tcacataaaa ggtggaattg acggcaatac ctatttggat
     1681 atgatgaacg agtcgcgtta catcacccct atgacaccgg taaaatcatc cttttcaatt
     1741 gttgatatcg aagccggagc aaagattggc gaagtcagtg gttttcgttt cgatattttc
     1801 ggaggattta agaaaaccga ggatgagcat ttcttggtat taaatcaaat agttcaggca
     1861 gagactcccg atatgccatc aattaaggaa tcattagccc ctctttatgg aaatttttct
     1921 cattctcata ttggaggaat gcttcaatcc aacatctggt cgcctttaga tattgcgttg
     1981 cgcataaaaa agaattttta taccgtaaat gacaccggta tccctgatgc aaaagcctat
     2041 aacaaaccgg gtttcgaaac tgatattagt actacgttta gtgccacgag caacctgaaa
     2101 ttcttgctca actattattt tgccggtgac aggtggtcct acttcaatga tacaaatgta
     2161 caaataaaca acattaacga cctcaatttt ggagcccttt acgacatcaa tgaatccttt
     2221 gctattcact tgaaagccaa caatcttctg ctccagaagt acgatatctg gtacggatat
     2281 ccggcacagg gtttcaatgc gatgggtggg tttacactta aattctaaat taaaagagtc
     2341 ggaacaacgg gctattacat aaaaatatcc ttaacgatac tgaagatgaa aaacagcaca
     2401 aaagttgtga agaaagggtg gttattgata ctgctgctga ctattctgca gcagcctgtc
     2461 gccggacaat tacaaaaggg gaagggttat cctaagaaag cctacgagag tgacctgcta
     2521 caacactttg ctgctccccc agggggttac ggcaatgttc ctttctactg gtggaacgga
     2581 gactcgctca ggaaagaacg gttgcaggag caactggaca tcctctcttc gtcagcagtg
     2641 gatggctttg ctgttagtta cattcatact tctccgcgca tagatacgtt ggagaacaga
     2701 gatggctacg gactctacgg tatgaccgaa ccgggtgtgc ctcacgtttt ttcggataaa
     2761 tggtgggagg tttggaattg gttctcgggt gaggctggac gccgtggcct gggagccgga
     2821 ctcgacgatt ataccgtggg ttggaacggt aacggatact atcccgatga gctggacacc
     2881 gtggcggtat tccgggatta cagcggtgag ctggtgatcc gcgtggacag catcagagga
     2941 ggagaaacct acaatgcctt gcagccagaa aacctgttga cggtagtagt atggccgggt
     3001 aaaatagtac ttacagacag cgtgacagac ggccgtatac gatggagggc tcccggcaag
     3061 ggaatcagtc gcatttacac cattactaca gcaccgggtt atgtgattca tcccgaacat
     3121 ggcaacaaat tattggatgt atatttcaat cgctttgagg agaggatgga tgatgcgggc
     3181 cgcgcgggga tgaactattt ttttcaggat gaactcgctt atccgatcca catgctcacc
     3241 tggtcggatg atttcagcga ggagtttatc cgcagaaaag gatatgacat cgttccctat
     3301 ctgcccgctt taaaagaatc cataggtccg gtcactcctc gcgtacgaat ggattattgc
     3361 gaggtattga tggatctttc cgaagaacgt tactacaagc ctatctatca gtggcatgcc
     3421 gacagaggat tgatgtacgg ttgcgacaat ctgagccggg gcaaggaccc aatggcatac
     3481 atagactact tccgggccat gtcgtggttc accgctcccg ggaacgacgc tcccgcaagg
     3541 ggatcgagct tcctcgaaac aaaaatttcc agttccatcg ctcatctcta tagccgtcct
     3601 cgtacctggc tggaggcgtt ccacagtatg ggatggggca gttcaggagc ctggcttacc
     3661 gatcagattg accaccactt cgttgccgga ggaaacctgg tgtgcatgca cggcttgtac
     3721 tattccacac acgggggatg gtgggagtgg gcgccgcccg actttcattt ccgaatgcct
     3781 tactggcctc acatgaaaag gtggctagac tataccgaac ggatgagttt cattctgagc
     3841 cagggctctc atgtttgtga tattgcgcta gtgtatccaa cggagacgat acaggcttat
     3901 cccggtacga aacccgacgg ggtgtttgat ctggccctaa ggctgagcaa cagcggactg
     3961 gattttgatt ttgtggattt tcgctcgttg agggatgcga gtattgagga gcgtgaactg
     4021 aggatggcgg acgaacggta caaagtgctt gttttggccg atatggaggc tatgcaccac
     4081 tcatccctta cccgggcgct ggcgttttac cgcgccggag ggattgtgct agctacaggt
     4141 cgcctgccga gggccacttc tgcaaaagga gagaaggacc ccgaggtaga ggcgatactg
     4201 cgtgaactat ttggccttac tgcagccgag gtggctgcgg gtaagccggc aaagaaacag
     4261 gtgaatgctg ccggcggtgt gggttggtat atccccgaag cacccgagcg gcaggttgcg
     4321 ggattgatta ctcccgactt ccggccggga cagggtgcgg gtaaggtact gcatcgcagg
     4381 gtgggcaaca aggatattta tatggtgaag gatgtggaga aggggagcga atgcttcttc
     4441 cgggctacgg gccgtgtgga gctgtgggat gcccacagcg gtaccgccca agccagtccc
     4501 gtgttgtctc aggataaggc cggcactcgc atccggatga acagagagac aggtaactcc
     4561 tacctgattg ttttctctcc gggcgaacca ctgatggcgg atgagggccg cacagccctc
     4621 cggcttagtt acgagatacc gttagggcgg gaatgggtga cggagttgct gcctacactt
     4681 cagaacaagt ggggagacta ccgctttccg gctttcgacg gtatgatagg agcggaggcc
     4741 cgctcgttcc gctatgcccc ggacaacggg atgccaggca actggatgca accggactat
     4801 aatgatgcca attgggagga gggtatttac ggctatggcc cgcaactcat gacccaggct
     4861 gacggtattg catggacacc ggtctccttc tcctggcagt acggcgtttg ggacaatccg
     4921 ggagcacagg gataccacgg cttgaaaggg aaggtggatg atcgtttctt tatattagac
     4981 aaagggaaaa atcagcgctt caagacgtat gtatatgccc cgaaaggtgg ggtttatagg
     5041 atagagtcag acggggtatt gccccaatat gtgcgtgtgg atgggcagga gatgaaagag
     5101 acggtccacc tgaagaaggg atggcatgct ttggaggtgc aatacgaccg gtcggagcag
     5161 ctagattata agccccgcac gggtttgtat aacgacacgc gtctgcgcgg aatggtgctc
     5221 ttctttccgg agaagacacc tctgccggag aaaccatccc cttatgccga tgaggtgtcg
     5281 tcgcgctggg ttacaggcaa acacctgctg tttgaccctt acggcggtac ccgcaaccgg
     5341 tggagcttcc gctttgacgc agtaccgggt ctggaggaga tgactctggt gttgcagggt
     5401 gaactgacgg acctctggat cgatggagag aaggccgcac ggagtcagat ccttcgtaca
     5461 cccggtaaaa gaggtatgaa ccgttatcgg gtgacactca ggaagaagaa agagcgaaca
     5521 gcaacggttg ccttcacggt gcgtgcggaa aaaggttacc agggtacggc tgtggtgcgg
     5581 gaaccggtga cactgaaaac ttcaacggga cttatgaaga gcggagactg gtcacaggag
     5641 ggagcgttac gatactattc cggagggatc cgttaccgtc aatcgtacga actgaataac
     5701 accgcggggg caaagcagat catgctgaaa ctgggagagg tggtggctac ctgcgaggtg
     5761 acagttaacg ggcagtcagc tggcgtgttg atcagccctc cctatgagtt ggacattacc
     5821 ggactggtga aagatggtaa gaacgatatc gaggtactgg tgtacagtac cctgtctaac
     5881 cactatcaga ctatccctac accgtaccgg ggtgagccgc gggccggact tatcggtccg
     5941 gtgttgatgt cggtatatga atgaatttac ggactcgggg cgagtttgtc cttttcattc
     6001 cttaactaca ttaagctgtg gttaaggaat accgaccgtg gtgaatttgt tgttggctgt
     6061 ccccggtgga ttcgttcgac aagttcctga catgttctgt aagccggtat tacggggaca
     6121 gaaccaagtt cccaggatgt attaacctga atatttgcgt ctttcgcatt cttcctgctt
     6181 cctgtgcgat acgtagcagt agaaaatcgg agtaagagga tacttcggga tctttcagcg
     6241 gatctccaaa ctcagcgaaa taggagaaaa ccagcctttt gtgtagttcc ctccccggga
     6301 atttcaaggg ttgacggagt ttatgactgt cctttcaaac agaatgccac aaacaataaa
     6361 caaaacatta ttttcttctt tatgattatc agtacgctat cgcatgcatt tttagctaca
     6421 gtgaatattc ggtatccagc cgttttatta tccttgcggg attccctgcc gctattacat
     6481 cgtcaggtat atctttagtt actacgctcc ctgcaccgat aattgttctg ttacctatgc
     6541 tgacccccgg gcagatggtt gtgtttcctc caatccacac attatttccg atgaagacag
     6601 gtttcccaaa ttcaattctc gtagctctct gcttccattg aaccggatgt gcagctgtat
     6661 atatttctac agaaggacct atcagtacat cgttaccgat agttacagtt gtcacgtcga
     6721 gtattgtgca gttataattg atgtagacgt tgtttccgaa gtggatgtta tatccgtagt
     6781 cgcagaaaaa aggcccttca atatgtacat tgtctccttg caccggacaa agttccttca
     6841 atatctcgga tctcttccct ttttcgacgg gatcgattgc gttgtactcc ctgcataatc
     6901 gtacagaatt caaatgttct ccggataatt cagcacctga acccatatac aattctccac
     6961 gaatcatttt ttccttttct gtcataaagc tgtttgaatg aatataattt tttgcggtaa
     7021 aaataaaaca attttctcag gttttggtac tttcttcaca aagaaagaat tcggatttaa
     7081 aaaaacttga ct
//
LOCUS       DAOSDR010000142         7090 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrimonas sp. isolate AMR_MDS_1826
            NODE_3873_length_7090_cov_3.648898, whole genome shotgun sequence.
ACCESSION   DAOSDR010000142 DAOSDR010000000
VERSION     DAOSDR010000142.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863355
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrimonas sp. (wastewater metagenome)
  ORGANISM  Petrimonas sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Dysgonomonadaceae; Petrimonas.
REFERENCE   1  (bases 1 to 7090)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7090)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:10:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,720
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 35
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7090
                     /organism="Petrimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3873_length_7090_cov_3.648898"
                     /isolate="AMR_MDS_1826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2023866"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..521
                     /locus_tag="PLI30_06805"
     CDS             <1..521
                     /locus_tag="PLI30_06805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="alanine-tRNA synthetase second additional
                     domain-containing protein"
                     /protein_id="HOI79298.1"
                     /translation="EIIVSRPSVFGPLPESIYQIVHTSLKFRKMAHTAEDVTMQTLEE
                     DYGITENRYFSSDVRNGFVLKFKEKPEINLDELEQRVKERLARHLEVSYFDEGHIRLG
                     DKVIPCDGSRFHVRNTSEITDFSLARHFIFDAKTNTHCLVGLIDKYTENLDNRNTQYF
                     LTRKCSGDNNEQ"
     gene            523..1368
                     /locus_tag="PLI30_06810"
     CDS             523..1368
                     /locus_tag="PLI30_06810"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006046380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase"
                     /protein_id="HOI79299.1"
                     /translation="MTRNISASAITRLVEQLCIEACCVLAGDINNKLKSCLQTETSPL
                     GKEILGTLIENARVAREESSPMCQDTGMTVVFVRMGQNVQVTGGFIEDAINQGVRQGY
                     EKGYLRKSVVKDPLRRENTRDNTPAVIHYEIVPGDVFHITVSPKGFGSENKSALKMLT
                     PSEGVEGVKRFVLETVSAAGGNPCPPIIVGVGIGGTMERAAYLSKKALLRPLDTQNPD
                     SALAALEAELLSEINKMGIGPAGFGGTTTALGVNILTYATHIAGLPVSVNIGCHATRH
                     AEGSL"
     gene            1389..1949
                     /locus_tag="PLI30_06815"
     CDS             1389..1949
                     /locus_tag="PLI30_06815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006046381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S-containing hydro-lyase"
                     /protein_id="HOI79300.1"
                     /translation="MMDKKILCAPFSDETIRSLKTGDMVYISGTVYTARDEAHRRLCE
                     MIRRGEPMPFDIEGQAVYYAGPAPAKPGKPIGSVGPTTGGRMDAYSPSLIARGLKVMI
                     GKGTRSAQVVDAMKQYTGVYFAAIGGAAALMAKCVESAEVIAFEELGTEAVHRLTVKE
                     LPVVVAIDCRGNDVYKLAREIYEQSI"
     gene            2023..2754
                     /locus_tag="PLI30_06820"
     CDS             2023..2754
                     /locus_tag="PLI30_06820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006844407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HOI79301.1"
                     /translation="MDIKNNITAVVKRIENACLSCARDPKEVKLLLATKTVIPDKIKI
                     AFEAGQTLIGENKVQEIKSKFNELAGFPHQKHFIGHLQTNKIKDILNHNVTCIQSLDR
                     LDLAEKLHQRLTFEKKEIDVLIQVNTSAEESKFGINPEDTVEFIKQVSLFENIHIKGL
                     MTIGLFSAETEKVRKCFRLLKQLQQETIRQNIPNVKMNELSMGMSGDLETAIEEGATI
                     VRVGTAIFGHRTYSDSYYWNENKRN"
     gene            2768..3424
                     /locus_tag="PLI30_06825"
     CDS             2768..3424
                     /locus_tag="PLI30_06825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI79302.1"
                     /translation="MRKICLLLLIILPVMMPAQVITYDTIRVSPDDERNIYKTQRQQQ
                     TAQSSIARQPVLLDRESAPGFRFDKSKLRYGANIGLSFSRNYSAFNFGPQVGYQLSSR
                     FMAGVGIKYYYNKVRAFQFNEEYLYKNNLLGANLFGYFYPARFLALFVQPEINRLWAN
                     LTDEMTGEVTKSSGFVPSLLVGGGLRLGHSHITLNYDLAQHTNSPYPRGWFLGFSTFF
                     "
     gene            complement(3421..3714)
                     /locus_tag="PLI30_06830"
     CDS             complement(3421..3714)
                     /locus_tag="PLI30_06830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006842206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF721 domain-containing protein"
                     /protein_id="HOI79303.1"
                     /translation="MLKKNAQPIATILSEFFDENPELKTSVAEHRAVSAWRELLGEGV
                     SHYTKNVYFRRNVLHVQLSSSVLRAELIMNKRNLIDKLNEHAGMEIVKDIVFR"
     gene            complement(3707..4813)
                     /locus_tag="PLI30_06835"
     CDS             complement(3707..4813)
                     /locus_tag="PLI30_06835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019540199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HOI79304.1"
                     /translation="MTLKHLSIINYKNLAQVELELSPKINCFVGNNGMGKTNLLDAVY
                     YLSFCRSYTNPVDSQIIKHDADVCMLQGRYELEGQEGEEEIYCGLRRRQKKQFKRNKK
                     EYEKLSDHIGFIPLVMISPADIEIIQGGSEERRKFMDMAISQFDKAYMHALIRYNNAL
                     QQRNATLKMDDNEIDFTLLELWEDQMAGEGELIFEKRQAFVKEFIPVFDEFYKRISLS
                     NEKATFDYVSQLRENNFREALRQTRRRDIAVGHTTTGIHRDELEMLLDGFPIKKVGSQ
                     GQNKTYFVSMKLAQFHYLLKTGKRTPILLLDDIFDRLDAYRVEEIVKLVSSNEFGQIF
                     ISDTNRGSFDKILERINNSHHIYSVKDGEITVYA"
     gene            complement(4819..5580)
                     /gene="cdaA"
                     /locus_tag="PLI30_06840"
     CDS             complement(4819..5580)
                     /gene="cdaA"
                     /locus_tag="PLI30_06840"
                     /EC_number="2.7.7.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019540198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004016 - adenylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diadenylate cyclase CdaA"
                     /protein_id="HOI79305.1"
                     /translation="MLEHFGIKDFIDILLVAALLYYLFKMVKISGMRPLFIGIVVFMI
                     IWVLVSQVFDMVLLGSILDQFVNIGLILLVILFQDEIRRFLMSLGSKKGWKFVSKLFI
                     PVDKQQKDTSHITSIVLACMNMSKARIGALIVVQGDLQLSLYEQTGEIINADISSRLI
                     ENIFFKNSPLHDGAMIVVGKKIKAAGCILPISQNPNIPKHMGLRHRAGLGITQETDAK
                     VIVVSEETGKITFAEEGRLKINVNPEELQQLILAE"
     gene            complement(5587..6420)
                     /gene="folP"
                     /locus_tag="PLI30_06845"
     CDS             complement(5587..6420)
                     /gene="folP"
                     /locus_tag="PLI30_06845"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005859770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HOI79306.1"
                     /translation="MIKSININGELLDFSTPLVMGILNVTPDSFYSGSRKQSEEEIVS
                     RVCQILEEGGKIVDIGGQSTTPTSTLIPAKEELKRLEPTLRLVRKEFPNTILSIDTFY
                     SEVAGPAVEKYGVNIINDISGGQIDDNMFETVARLNVPYILMHMRGTPQTMQQHTHYD
                     NFIQDILYYFSEKISKLNLLGVNDIIIDPGFGFSKTTDQNYQLMAYLKYFNIFDVPIL
                     VGISRKSMIYKLLECTPQESLNGTSALNTFALLSGANILRVHDVKEAVECVKIVEKIS
                     K"
     gene            complement(6442..>7090)
                     /locus_tag="PLI30_06850"
     CDS             complement(6442..>7090)
                     /locus_tag="PLI30_06850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019540196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HOI79307.1"
                     /translation="THKDAEETKKHFPTIYEKCLTYGIDITKEMIPVAPAAHYLCGGI
                     FVSIDGETSINRLYANGECACTGLHGANRLASNSLIEAVVFADSAAKHSVPLFKKFSF
                     QEDIPDWNAEGTTSTEEMVLITQSYKEVGQIMSSYVGIVRSDIRLQRAMDRLNILFRE
                     TENLFQRSVVSREICELRNIIKVGYMVIKQAMARKESRGLHYTIDYPDKDPDSTL"
BASE COUNT         1887 a         1645 c         1682 g         1876 t
ORIGIN      
        1 aggagattat tgtgtcgcgc cctagtgttt ttgggccgct tcccgaaagt atttaccaaa
       61 ttgtgcatac ttcgctcaaa ttccgcaaga tggcccatac agccgaagac gtaaccatgc
      121 agacattgga agaggactac ggaattactg aaaatcgtta tttctcatcc gacgttcgca
      181 atggctttgt cctgaaattc aaggagaagc ccgaaatcaa cctcgacgaa ctggagcaga
      241 gagtaaaaga acgcctggcc aggcacctcg aggtgtccta tttcgacgaa gggcacattc
      301 gtttgggaga caaggttatc ccgtgcgacg gttcccgatt ccatgtgaga aatacatcgg
      361 agattaccga tttttcgctg gccaggcatt tcatctttga tgcaaagacc aatacccact
      421 gcctggttgg attgattgac aagtacaccg agaacctcga caaccgcaat acgcagtatt
      481 tccttacccg gaaatgtagt ggcgacaata acgaacaata gcatgacaag aaacatatcc
      541 gcatccgcta tcacccgtct ggtagagcaa ctttgcattg aagcttgctg tgttcttgct
      601 ggagatataa acaacaagct taaaagctgc ttgcaaaccg aaacatcacc gctcgggaaa
      661 gaaattctgg gcacgcttat tgaaaatgcc cgtgttgccc gggaagagag cagcccgatg
      721 tgccaggaca cgggaatgac cgtcgttttt gtacgcatgg ggcaaaacgt gcaggttacg
      781 ggtggattca tcgaagatgc cattaaccag ggtgtacggc agggatacga aaaaggatac
      841 ctgcgcaaat cggtcgtgaa agacccgctc cgccgcgaaa atacccggga caatacaccg
      901 gccgttattc attacgaaat cgtccccggt gatgtttttc atataactgt ttctcccaaa
      961 gggtttggca gcgaaaacaa aagtgcatta aaaatgctca cacccagcga aggcgttgaa
     1021 ggcgtgaagc gatttgtact ggagactgtt tcggctgccg gagggaatcc ctgcccgccc
     1081 attatcgtgg gtgtgggcat tggcgggacg atggaacggg cggcttattt aagcaagaaa
     1141 gcgttgctgc ggccgctgga tacacaaaac cccgattccg cacttgccgc tttggaagca
     1201 gaattgcttt cagaaatcaa taagatggga ataggccctg ccggtttcgg aggaaccaca
     1261 accgcactcg gggtaaatat actgacctat gctacccata tcgccgggct tccggtttcg
     1321 gtaaatatcg gctgccacgc cacccggcat gcggagggaa gtttgtgaac agaagccaac
     1381 aaaaacgtat tatggataaa aaaattcttt gtgccccctt ttccgacgaa accatccgtt
     1441 cgttgaaaac gggcgatatg gtctacattt cgggaactgt ttatacagcc cgcgatgagg
     1501 cgcacagacg attgtgcgag atgatccgcc ggggtgagcc gatgcctttt gatattgaag
     1561 ggcaggccgt ttactatgcc ggtccggctc ctgccaaacc gggcaagccc ataggatcgg
     1621 taggccccac taccggtggg cggatggacg cttattcgcc ctccctcatt gcccgcggcc
     1681 tcaaggtgat gatcggtaaa ggaacacgca gtgcccaagt ggtagatgcc atgaaacaat
     1741 acacgggcgt ttattttgct gcaatcggtg gcgccgccgc cctcatggca aaatgcgtgg
     1801 aaagtgcaga ggttatcgct tttgaagagt tgggcacgga ggctgttcac cgcttaactg
     1861 tgaaagaact tcccgtggtt gttgcgatag actgtcgggg aaacgatgtg tataaactgg
     1921 cccgggagat atacgaacaa tcaatttaaa ataccgggaa taaaataagt tgcgtatatt
     1981 tgttcttcgg agagatccga acagtaaatc cgaaacttat atatggacat caaaaacaat
     2041 ataaccgctg tagttaaacg gattgaaaac gcttgcctga gttgcgccag agatccaaaa
     2101 gaagtgaagt tgctgttggc aacaaagact gtaattccgg ataagataaa aatagccttt
     2161 gaagccggac aaacattaat cggtgaaaac aaagtgcagg agataaaatc gaaattcaat
     2221 gaattagccg gttttccgca tcaaaaacac tttatcggac acctgcaaac caacaaaatc
     2281 aaggacattc tcaaccacaa tgttacctgc attcagtcgt tagaccggtt ggatttggca
     2341 gaaaaattgc accaacggtt aacttttgag aaaaaggaaa tagatgtcct tattcaggta
     2401 aacacttctg ccgaagaaag taaattcgga atcaatcctg aagacaccgt tgagtttatt
     2461 aaacaagttt ctctattcga aaatattcac atcaagggat taatgaccat tggtttgttc
     2521 agcgccgaaa ccgaaaaggt aagaaaatgc ttcaggctat tgaagcagtt acagcaggaa
     2581 accattcgtc agaatatccc gaacgtgaaa atgaatgaac tgtccatggg aatgagcgga
     2641 gacttggaaa ccgccattga ggagggagcg accatcgtga gggtgggcac cgccattttc
     2701 ggccacagaa cttattccga tagctattat tggaatgaaa ataaacggaa ttaaaaccaa
     2761 aaactctatg agaaagatat gcctcctttt gttgatcatt cttccggtaa tgatgccggc
     2821 tcaggtgatt acttacgaca caatccgcgt ttcaccggat gacgagcgga atatttacaa
     2881 gacacagcgt cagcaacaaa ccgcccaaag ctccatagcc cgacagccgg tacttttgga
     2941 tcgggaatcg gctccggggt tccggttcga taaaagcaaa ctccgttacg gggccaatat
     3001 cggactgtct ttcagccgga attattcggc ttttaatttc gggccacagg tcgggtatca
     3061 gctgagcagc cggttcatgg caggggtagg gataaagtac tactacaata aagtgcgggc
     3121 atttcaattc aacgaagagt acctatacaa gaataatctg ctcggcgcta atctgttcgg
     3181 atatttttat ccggctaggt ttctggctct gtttgtccag cctgaaataa atcgtttgtg
     3241 ggcgaacctg accgacgaaa tgacgggtga agtgactaaa agcagcggtt ttgtgccttc
     3301 attgctggta gggggaggcc tgaggttagg gcattcccac attacgctga actacgatct
     3361 ggcacaacac accaactctc cctatccgag gggctggttc ctgggatttt caaccttttt
     3421 ttaacgaaaa acaatatcct tcacaatctc cattccggcg tgctcgttca acttgtcaat
     3481 caggtttcgt ttgttcataa tcaattcggc acgcaacacc gatgaggaaa gctggacgtg
     3541 taggacgttc cgccggaaat agacgttttt ggtgtagtga gaaacccctt cgcccagcaa
     3601 ttccctccat gccgagacgg cacggtgttc agccaccgat gtcttcaatt ccgggttctc
     3661 atcgaagaat tccgaaagga tggttgctat aggttgggcg ttttttttaa gcataaactg
     3721 ttatttcccc gtctttcacc gaatagatgt ggtggctgtt gttgatccgt tccagtattt
     3781 tatcgaacga cccccggttg gtatcggaga taaaaatctg gccaaattca ttgctcgaaa
     3841 ccagtttgac aatttcttcc acccggtagg catccagcct gtcgaagata tcgtccagca
     3901 gcaaaatggg tgtccgtttc ccggttttaa gcaggtaatg aaattgtgcc agcttcatcg
     3961 atacgaagta ggttttgttt tgcccttgcg aacccacttt ctttatggga aatccgtcga
     4021 gcaacatctc cagttcatcg cggtgaatac cggtggtggt gtgtcccacc gcgatgtcgc
     4081 gacggcgggt ttgtcgcagc gcttccctga aattgttttc gcgaagttgc gaaacgtagt
     4141 cgaaagtcgc cttttcgttg gataaactta ttcgtttgta aaactcgtca aaaacgggaa
     4201 taaactcttt tacaaacgct tgtcgttttt caaaaatgag ctccccttct cccgccatct
     4261 ggtcttccca aagctccagc aaggtgaaat cgatttcgtt gtcgtccatt ttcaatgttg
     4321 cgttgcgttg ttgcaaggca ttgttgtacc ggatcaaggc gtgcatatac gctttgtcga
     4381 actgagaaat agccatgtcc ataaatttcc gtcgttcttc gctacccccc tggattatct
     4441 cgatatcggc aggagaaatc atcaccagtg gaataaatcc aatgtggtcg gagagttttt
     4501 cgtactcctt tttgtttctc ttgaactgtt ttttctgccg ccggcgcaat ccgcaataga
     4561 tttcttcctc gccttcctgt ccctccagct cataccttcc ctgaagcatg caaacatctg
     4621 cgtcgtgttt tatgatctga ctatcgacgg ggttggtgta actccggcag aacgacaggt
     4681 agtaaacggc gtccagcaaa ttggttttac ccatgccgtt atttcccaca aaacagttta
     4741 ttttgggcga taactccagc tcaacctgag ccaggttttt ataatttata atggataaat
     4801 gctttaatgt cattgtgatt attccgccag gatcagttgt tgcagctctt cgggattaac
     4861 gttgattttc aaccgccctt cctcggcaaa tgttattttt ccggtttctt ccgaaacgac
     4921 aatcactttg gcatcggttt cctgcgtgat ccctaatccg gcacgatgcc gtaaccccat
     4981 gtgtttgggt atgttcgggt tctgcgatat gggaagaata cagcctgcag cttttatttt
     5041 tttccccaca acgatcatgg ctccatcgtg caagggactg ttcttgaaga aaatattttc
     5101 aatcagccgg gaggagatat cagcgttgat gatttcgccc gtctgctcgt ataagctgag
     5161 ttgtaggtcg ccctgaacga cgatgagcgc gccaatcctg gctttcgaca tgttcatgca
     5221 ggccagtacg atcgaagtaa tgtgcgatgt gtctttttgt tgcttgtcga ccgggataaa
     5281 gagtttcgat acaaacttcc accctttctt cgatcccaga gacataagga acctacgtat
     5341 ttcatcctga aaaagaatga ccagcaatat caggccgata ttcacgaact ggtcaagaat
     5401 agagcctaga agcaccatat cgaatacctg tgatacaagt acccagataa tcataaagac
     5461 aacgataccg atgaaaagcg ggcgcatccc cgatattttc accattttaa acaggtagta
     5521 caacagggct gctaccagta aaatgtcaat aaaatctttt attccaaagt gttcgagcat
     5581 acaacgttat ttgcttattt tttccacaat ctttacacat tccacggctt ctttcacgtc
     5641 gtgaacgcgt aagatgtttg ctcccgacaa caaggcaaat gtatttaaag ccgatgtacc
     5701 gttcaggctt tcctgtgggg tacactccag cagcttgtaa atcatcgatt tccgagagat
     5761 gccaaccaga attggtacat caaagatatt gaaatatttt aaataagcca tcaattggta
     5821 gttttgatca gtcgttttac tgaatccaaa ccccgggtca atgatgatgt cgtttacgcc
     5881 caacaggttc aatttcgata ttttctcgga aaaatagtac aggatatcct gaataaaatt
     5941 gtcgtaatgg gtgtgttgtt gcatggtttg cggtgtacca cgcatatgca tcaggatata
     6001 aggaacattg agccgggcaa ctgtttcgaa catgttatca tcgatctgcc cgcccgaaat
     6061 atcgttgatg atgtttaccc cgtatttttc cacggcaggc ccggctactt cggaataaaa
     6121 cgtatcgatg gaaaggatag tattgggaaa ttcttttcga acgagccgaa gtgtcggctc
     6181 taagcgtttc agctcttcct ttgccggtat taaagtggaa gtcggtgttg tggattgacc
     6241 gccaatatcg acaattttcc caccttcctc cagaatttgg cagacgcggc tgacaatctc
     6301 ttcttcagat tgtttacgac tgcccgaata aaaggaatcg ggagtaacat ttaaaattcc
     6361 catcaccagc ggagtggaaa aatcgagcaa ttcgccgttt atgttaatgg attttatcat
     6421 accttgtgtt gtgtattacg gctataacgt tgagtccgga tccttgtccg gatagtcaat
     6481 ggtatagtgc aggccgcggc tttctttgcg tgccatggct tgcttaatga ccatatagcc
     6541 taccttgatg atattgcgga gttcacaaat ctcgcgcgag acaaccgacc gctgaaaaag
     6601 gttctcggtt tcccgaaaaa gaatgttcag cctgtccatt gcccgctgca ggcgaatatc
     6661 ggaacggacg atccccacgt aggacgacat gatctgaccc acctccttgt aactctgtgt
     6721 gatcagcacc atctcttcgg tggaggtggt cccttcggca ttccaatcgg gaatatcttc
     6781 ctgaaaggaa aactttttga ataggggtac agagtgcttg gcagccgaat cggcaaaaac
     6841 cactgcttcg atgagtgagt tagatgccag ccggttggct ccgtgcaagc ctgtacaggc
     6901 acattcgccg ttggcgtaaa ggcggtttat gcttgtttct ccgtcaatac ttacgaaaat
     6961 tccaccgcac aaataatggg cagccggggc taccggaatc atctctttag taatatcgat
     7021 accgtaggta aggcatttct cgtaaatggt ggggaaatgt tttttggtct cttcagcgtc
     7081 cttgtgcgtt
//
LOCUS       DAOSDR010000143         7088 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrimonas sp. isolate AMR_MDS_1826
            NODE_3876_length_7088_cov_3.610266, whole genome shotgun sequence.
ACCESSION   DAOSDR010000143 DAOSDR010000000
VERSION     DAOSDR010000143.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863355
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrimonas sp. (wastewater metagenome)
  ORGANISM  Petrimonas sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Dysgonomonadaceae; Petrimonas.
REFERENCE   1  (bases 1 to 7088)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7088)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:10:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,720
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 35
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7088
                     /organism="Petrimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3876_length_7088_cov_3.610266"
                     /isolate="AMR_MDS_1826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2023866"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            26..901
                     /locus_tag="PLI30_06855"
     CDS             26..901
                     /locus_tag="PLI30_06855"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HOI79308.1"
                     /translation="MPSLKEIKTRIQSVRSTRKITSAMMMIASSKLRKIQKIIENLYP
                     YQQKLQQLMELFVNSQDNLVSPFAERRQIKRVALILFSSNTSLAGRFNENVISQLKVT
                     VNEYLPLGKENIHIYAIGDKVYEAARKLGFETPNNFRHIADKPSYEATRQLASDLMEL
                     FLNKKIDKVELIYHHYKSKGTQELLRETFLPITLMTEDERWKIPQPDYIVEPDPQTIM
                     DQLIPKVLKLKLYTIHADSVSSEHAARTIAMQTATDNADDLLDELTLQYNKLRQQSIT
                     NELLDIIGGSFGKPG"
     gene            complement(923..1615)
                     /locus_tag="PLI30_06860"
     CDS             complement(923..1615)
                     /locus_tag="PLI30_06860"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HOI79309.1"
                     /translation="MNPFPESDTDNCEKMVACFRDLKPEELTLISSGKTELTYLAGET
                     LFKQGAFSTHVLLVMDGLVKVYLQAGRDKQLNLQLAKPGDFLAFASVFGETVHTTSAI
                     ALTDTQVCMIDKENMRQLLMNNAEFAMRITSRNFRDERQLLTVIGNITYKQMRGKLAS
                     ALLYLSGDEFSGQEVFPHLSRQDIANFASIATESTIKFLKEFERDGIIVLEGKDITIL
                     DRSELEKTERLG"
     gene            1791..2138
                     /locus_tag="PLI30_06865"
     CDS             1791..2138
                     /locus_tag="PLI30_06865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019540694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HOI79310.1"
                     /translation="MKYLRTNIHEIETAEELQKIIDENENVMVCCGRMGPMCIPVYEI
                     MEELEEERPEVKFYSMAFDSPESGVIRNAPECRGFMGLPFTMYYKSGKVAKVTTSIQN
                     MQQITSNLDQFLS"
     gene            2211..3137
                     /gene="trxB"
                     /locus_tag="PLI30_06870"
     CDS             2211..3137
                     /gene="trxB"
                     /locus_tag="PLI30_06870"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019540693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HOI79311.1"
                     /translation="MEKIYDVMILGAGAAGLSAGIYASRGKLDTLILSEGVTGGQMVL
                     TNEIANYPGVETIKGYELVDIMRRQAKSFGCTIKTNVKIVRYQLAGETKEVELEDGRI
                     FRAKSVILAPGGKPRSLDIPGEKEFEGRGISYCATCDADFFQDKRLIVVGGGNSALEE
                     AVTLTNFASHVTIVHQFDHFQAFAHAVEEAKQNPKISFVMESELRAYMGNGTLKKVQI
                     ENLKTKAVSELETDGVFVFIGYKPNTSAFKGMVEINERDEVIVDPAMRTNIPGVFAAG
                     DSIAKRYRQVTTAVSDGTIAALSATEYVRAKE"
     gene            3726..4745
                     /locus_tag="PLI30_06875"
     CDS             3726..4745
                     /locus_tag="PLI30_06875"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOI79312.1"
                     /translation="MKKYNVGIVGYSWAAGAHIDAINRTSLAQVTAICSSRGLDSKEI
                     SARHGGNIRCYEDYSEMIANPDIHVVSICSYPALHAKQAIMAARAGKNLIIEKPIALT
                     WEDCLAVEKAVKEAGVKTCVCFECRFSGQLQTVKSLIEQGLLGKIHYAEVDYYHGIGP
                     SYGQYRWNIKKEEAGSSLLSAGCHAMDALLYCIDSEVDAVHSYSTGSSHADFAKYEYP
                     TSSVSIIKFKNGAVGKVASVIDCLQPYYFHFHLVGSEGSLLDNKFYSTKIKELNKNKW
                     SELSMKLLDSGDVSDHPYLHQFEAFFKAMDEGREMPLTSLADAMKTHELIFAADKSAE
                     ENNLK"
     gene            4755..5513
                     /locus_tag="PLI30_06880"
     CDS             4755..5513
                     /locus_tag="PLI30_06880"
                     /inference="COORDINATES: protein motif:HMM:NF014625.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HOI79313.1"
                     /translation="MDLEKRIVAILAHPDDAEFMCAGTLSLFRNRGWEVHIVTKTPGD
                     KGSAVHTREEISRIRTGEARRSAQIIGATYHCVGLEDVYIIYDREAINRTTAVLRKIR
                     PSIVITASPTDYMVDHETTARIVQTACFCCGIKNMEVDEAPFEPSPYLYYADAMDGKD
                     KFGHAVAPSMYVDITGEMPVKEEMLKAHASQRDWLLEHHKVDEYILSMKHFAEVRGKE
                     VNRKYAEGFRQHLGHGFQQGNVLAGILEENIIIK"
     gene            5540..6472
                     /locus_tag="PLI30_06885"
     CDS             5540..6472
                     /locus_tag="PLI30_06885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI79314.1"
                     /translation="MSRKISMLAPDWWDFTTLEDEILGDAARLTPEDMLALSRPGFKV
                     VFYDTLEDFYLAEALEYITAWKQATASEPVGVCGPIGPTEQLPLVARLVNELELDLSN
                     AHFWGMDEWYVDGKEIGVESPISFERANNELMFHRMNKKLRMPEENIHYPEADTSKYI
                     KSWNTGVRCAVMQGGQGDTKHWAFNDPVKREGKYKDNPPSLEEYKKLTTRVVDLHPIT
                     CMQNARTSAGGVVTGIPSQALTVGPVQTWQAEKVSIWQAGVHDNPFGQRLTALMIGKK
                     IIDSAVPMSLLADHPNVQFNYYRGGLGTCGTEMH"
BASE COUNT         2125 a         1554 c         1649 g         1760 t
ORIGIN      
        1 cgacaccaag aaacagttgt agccaatgcc ttcgttaaag gaaataaaaa cacggataca
       61 gtcggtgcgg tcgacacgaa aaatcacgtc ggccatgatg atgatcgctt cttccaaatt
      121 acggaaaata cagaaaatca tagaaaacct gtatccgtac caacagaaac tgcaacaatt
      181 gatggagctt tttgtgaact cacaagataa cctggtttcc ccatttgcag aacgacgtca
      241 gataaagcgg gtggcactta tcctgttttc gtccaacaca agccttgccg gcagattcaa
      301 tgaaaatgta atctcccaac tgaaggttac ggtaaacgaa tatctccctc taggaaaaga
      361 gaatatccac atttatgcca ttggtgataa agtgtatgaa gcagcccgga aattggggtt
      421 tgagactcca aataattttc gccacatcgc cgacaagccc tcgtatgaag caacgcggca
      481 actggcctcg gacctgatgg aacttttttt aaacaagaag atcgataaag tggagttaat
      541 ttatcatcac tacaaaagca aaggaacaca ggaactgtta cgggaaacat ttttacccat
      601 taccctgatg acagaagatg aaagatggaa aattccccag cccgattata tcgttgagcc
      661 tgatccacaa accatcatgg accagctcat ccctaaagta ctgaaattga aactatacac
      721 tattcacgca gattcagtat catcggaaca tgccgcacgt actatcgcca tgcaaaccgc
      781 caccgacaat gccgatgatc tgctggatga actcacactc caatacaata aactacgcca
      841 gcaatccatt actaacgagc tgttggatat cattggcgga tcgtttggaa agcctggata
      901 atttttgaaa gtgaaccttt tgttaaccca accgctctgt tttttccaac tccgaccggt
      961 caagtatagt gatgtctttt ccttcaagca ctataatccc atcacgctca aactctttta
     1021 gaaacttgat ggtactctcc gtggctatgg aagcaaaatt ggcaatgtcc tgccgggaaa
     1081 ggtgcggaaa aacttcttgt cccgagaatt catctccgct aagatacaac agtgccgaag
     1141 ccagtttgcc ccgcatctgc ttatacgtga tattaccgat aaccgtcaac aattgacgct
     1201 catcgcgaaa atttcgcgac gtaatgcgca tggcaaactc cgcattgttc atcaacaatt
     1261 gacgcatgtt ttccttatca atcatgcaaa cctgtgtatc cgtcaacgct atagccgatg
     1321 tagtgtgtac cgtttcccca aaaacagagg caaacgccag aaaatctcct ggttttgcca
     1381 gttgtagatt cagctgctta tcgcgtccgg cttgtaggta aacctttacc aacccatcca
     1441 ttaccaacaa gacatgggtg gagaatgcgc cctgtttaaa caacgtttct cctgccagat
     1501 aggtgagttc tgttttaccg gaactgataa gcgtcagctc ctcaggcttc aagtcgcgaa
     1561 aacaagctac catcttttcg caattatctg tatcactctc tggaaatggg ttcatttttc
     1621 ttactttttc catcaaagat acaacctttt catataactg acatttatca gtccaaaaac
     1681 tgacaaagct catttttttt catggagtat gtcagccggt attcttcgtt ttcaaaccga
     1741 ttatgccata actttgtcgt cggaaaataa caataaaatc aacaataatt atgaagtatt
     1801 taagaactaa catccacgag attgaaactg ctgaagagct gcagaaaatt atcgacgaga
     1861 acgaaaacgt aatggtatgc tgcggacgta tgggaccgat gtgtatccct gtttatgaaa
     1921 tcatggaaga actggaagaa gaacgccctg aagtgaaatt ctactccatg gcttttgaca
     1981 gcccggaatc gggagttata agaaatgctc ccgaatgccg cggatttatg ggacttcctt
     2041 tcaccatgta ttataaaagc ggaaaggtag ccaaggtaac caccagcata caaaatatgc
     2101 agcaaatcac atcgaatctg gatcagtttc tgtcgtgata attatttttt gtattaattt
     2161 catgaagatc aatccccaaa aggattggtc ttccttttaa aagaaagagt atggagaaaa
     2221 tatacgacgt aatgatcttg ggagccggag cggccggatt gtctgcggga atctatgcct
     2281 cgcgcggaaa actggatacg ttgatcttaa gcgaaggcgt cacgggagga caaatggtgc
     2341 tgaccaacga aatagccaat taccccggtg tggagacgat aaagggatac gaactggtgg
     2401 atattatgag gaggcaggcc aaaagttttg gctgcaccat caagacaaat gttaagattg
     2461 tacgttacca attagcgggt gaaactaaag aagtagaact ggaggacgga agaatcttcc
     2521 gggcgaaatc ggtgatcttg gcccccggag ggaaaccccg ctcgctggat attcccggtg
     2581 aaaaggagtt cgaaggacgg ggaatctcct attgtgctac gtgcgatgcc gacttcttcc
     2641 aggacaagcg gttgattgtg gtgggaggcg gaaactcagc tctggaggaa gccgtaaccc
     2701 taaccaactt tgcatcgcac gtaacgatag tgcatcagtt tgaccatttt caggcatttg
     2761 ctcatgctgt ggaagaggcg aagcaaaacc cgaagatcag ctttgtgatg gaatcggagc
     2821 ttcgtgccta tatgggcaac ggaacgttga aaaaggtgca gatcgagaac ttaaaaacca
     2881 aagcggtctc cgaattggag accgatggcg tttttgtttt tatcggctac aaacccaata
     2941 cctccgcatt taaaggtatg gtagagatca acgaaaggga cgaggtcatt gtggatccag
     3001 caatgaggac caatatcccc ggcgtttttg ccgcaggcga cagtattgcc aaacgctacc
     3061 ggcaggtaac cactgcggtg agtgacggca caattgccgc actcagcgcc accgaatacg
     3121 taagggccaa agagtaaatt tcctttcgaa tttaacaaac acaataaagt cctgaaagcc
     3181 aaatgcttta cagggcctta ttgtgttata ataccttgtg ccttactgtt gtttatagcg
     3241 gtctgaatct atgccggcaa gttaattcaa taaattactg atttgttatg ctttttttat
     3301 acatttgcag aaccaacaat aaataattga accttcccaa acaaaacgac gtactgtgaa
     3361 acaatggttc aacgatactt tcaaagacaa acagcttctt tactttagta aaaaggcgat
     3421 agaatcaatt tttcagtttc gcaagctttt taactttttc ggacaaaaag cttggttttt
     3481 ctttaaccta tgacttgtag taaagcaatc ataaggttga atcttttttt acgaaattct
     3541 caacataaac accgtaagta tttataaaat aattactccc tgaaccaaga aatatcaatg
     3601 aacaaagcat ttaaatttaa caggacttgt caaaagagaa tgagtgagtt tgccatagtt
     3661 gaaattatta taaaacaata catgaaatca attaaaacaa tttataaata taaattcaac
     3721 gtattatgaa aaagtataac gtaggaatcg ttggttacag ttgggctgca ggcgcccaca
     3781 tagacgcaat taataggacc tctcttgcac aggttactgc gatctgttct tcgcggggac
     3841 tggattcgaa ggaaatcagc gccaggcacg gagggaacat caggtgttac gaggattatt
     3901 cggaaatgat tgccaatccc gatatacatg tggtgtccat atgcagttat cctgccctcc
     3961 acgctaagca ggccattatg gcagcccggg ccggcaaaaa cctgatcatt gagaaaccga
     4021 tagccctcac gtgggaagat tgtctggccg tggagaaggc tgtgaaggaa gctggcgtaa
     4081 agacatgcgt ctgcttcgag tgccgttttt ccggtcaatt acaaactgta aagtcactta
     4141 tagaacaagg attattgggg aaaattcatt atgcggaagt tgattattac cacggtatcg
     4201 ggccatctta cgggcagtac cggtggaata ttaaaaaaga ggaagcggga agtagcttgt
     4261 tgtcggcagg gtgtcatgcc atggatgccc tcttgtactg tatcgacagt gaagttgatg
     4321 cggttcatag ctactcgacc ggctcttccc atgccgattt tgccaaatat gaatatccca
     4381 ccagctccgt ttcgatcata aaattcaaaa acggtgctgt aggaaaagtt gcctcggtta
     4441 ttgattgtct gcagccctat tatttccatt tccatttggt aggaagtgaa gggagccttt
     4501 tggataataa attttattct acgaaaatca aagagttaaa caagaataaa tggagtgaat
     4561 tatccatgaa gctgcttgat tcgggcgatg tgtccgatca cccatacctg catcagtttg
     4621 aagcattttt taaagcaatg gacgaaggca gggagatgcc tttaaccagc ttggcggatg
     4681 ccatgaaaac acacgaactg attttcgccg ctgataaatc cgccgaggaa aataatctta
     4741 aatgaaataa caggatggat ctggaaaaaa gaattgttgc cattttagcg catccggacg
     4801 atgcggaatt catgtgtgcc gggacattgt ccctgtttag aaacaggggc tgggaggttc
     4861 atattgtcac caagactccc ggagataaag gctctgcggt acacacccgg gaggaaattt
     4921 cccggataag gaccggcgag gcacgcagat ccgcgcagat tatcggggca acctaccatt
     4981 gcgtggggct cgaagatgtg tacataatct acgataggga agcgataaac aggacaaccg
     5041 ccgtgttgag aaagatccgg ccctcgatag ttattaccgc cagcccgacg gactacatgg
     5101 tggatcacga gacgaccgca aggattgttc agacggcctg cttctgttgt ggcataaaaa
     5161 acatggaagt tgatgaagcg cccttcgagc cgtcacccta cctctattac gcggacgcca
     5221 tggatggaaa ggacaagttc ggacatgccg tcgccccctc gatgtatgtg gacataacgg
     5281 gagagatgcc ggttaaggag gagatgttga aagcccatgc aagccagcgg gactggctgc
     5341 ttgaacacca caaggtagac gagtacatcc tttccatgaa acactttgcg gaggtaaggg
     5401 gaaaagaggt gaaccgcaag tacgcggaag gtttccggca acacctgggg cacgggttcc
     5461 agcagggaaa cgtgcttgcc gggatcctgg aagagaatat cattatcaag taaaacaccc
     5521 aaattaaata atttaaacta tgtcacgaaa aataagtatg ctggccccgg actggtggga
     5581 tttcacaaca ctggaagacg agattctggg cgatgccgcc aggttgaccc ccgaagatat
     5641 gctggccctt tcaaggcccg gattcaaggt tgttttctac gacacgctgg aggacttcta
     5701 cctggccgag gcgcttgaat acattacagc atggaaacaa gctacggcaa gtgaacccgt
     5761 cggggtctgc ggccccatag ggccaaccga acagctcccg ctggtggccc ggctggtgaa
     5821 cgagctggaa ctggatctgt caaatgccca tttctggggt atggatgaat ggtacgtgga
     5881 cgggaaggag atcggcgtcg agagccccat ctcgttcgaa agggcgaaca atgagctgat
     5941 gtttcacaga atgaataaaa aactgcgcat gccggaggaa aacatacact atcccgaagc
     6001 cgacacctcg aaatacatca aaagttggaa cacgggcgta cgatgtgcgg taatgcaggg
     6061 cggacagggt gacacgaaac attgggcctt taatgatccg gtgaaaagag aagggaaata
     6121 caaggacaac cctccctcgc tggaagagta caagaaactg accacccgtg ttgtcgacct
     6181 gcatcccatt acctgtatgc aaaacgcgag gaccagtgcc ggcggtgttg ttacgggaat
     6241 cccctcgcag gccttgacgg tgggccccgt acaaacctgg caggccgaaa aggtatcgat
     6301 ctggcaggcg ggagtacatg acaatccgtt tggacagagg ctgaccgcat tgatgatcgg
     6361 gaaaaagatt attgactccg ccgtacccat gtcgctgctg gcagaccacc ccaacgtcca
     6421 gttcaactac tacagggggg gactgggtac ctgcggaacc gaaatgcatt aacatgctgg
     6481 cgaagaaaaa cgaataggca attgcctttg tctatcaagg aggatagatc tggagcgttg
     6541 tccacgttta tttacaggca cgcaaaaaga ggccgtctca taataaacta tgagacgact
     6601 tttttctgtc ttcttttagt agataataac atctaaaaag ataaaaatag cccctgataa
     6661 accttataat gccactttag caggttatgt gcaatcgaga acaatacata cttcacttct
     6721 gccctgtctt tacccctgta gaagaaccgt ttgcaatttt ttgtttttca gggtaccgaa
     6781 agcagcctcc tccttcgggt aacaactgta aagaattcac gctacacaag atgatcgatg
     6841 tatgtgatgg tgtcggaagt ggtctggtga aggcggatta tttttgcgga attatttcac
     6901 ctggatgagg tggaagtagt acattgtcgt tggtctgtaa aatttaacat tacttctgca
     6961 ccatttcaaa tagtcttaaa atctctaatt tttaagcaac taggcaattt ttcgttcatg
     7021 aaaatgaaaa ccgtgaaaca gcctctttat cacttaaaaa tgattcgctt tttcaaccaa
     7081 tatgcaag
//
LOCUS       DAOSDR010000144         7036 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrimonas sp. isolate AMR_MDS_1826
            NODE_3906_length_7036_cov_4.422146, whole genome shotgun sequence.
ACCESSION   DAOSDR010000144 DAOSDR010000000
VERSION     DAOSDR010000144.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863355
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrimonas sp. (wastewater metagenome)
  ORGANISM  Petrimonas sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Dysgonomonadaceae; Petrimonas.
REFERENCE   1  (bases 1 to 7036)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7036)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:10:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,720
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 35
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7036
                     /organism="Petrimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3906_length_7036_cov_4.422146"
                     /isolate="AMR_MDS_1826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2023866"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..717)
                     /locus_tag="PLI30_06890"
     CDS             complement(<1..717)
                     /locus_tag="PLI30_06890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005681302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HOI79315.1"
                     /translation="MANKKNFIIDTNVILHDYSFIENFEENDIYIPFVVLEELDKFKK
                     GNEQINFNARAFVRELDLITDDNLFKQGADLGVGRGKLYIVNSVKTHDKIIEAFPERT
                     PDNRILSTVLDVTEKHPKMKTILVTKDINLRMKARSLGIPVEDYINDKVIDIDIFGRG
                     EQVIEGLNPELIDKIYAQPTGVDVDEFTFDNPLVPNDSFVLKSERNSVLARYNPFTQK
                     IIRVDKEPSFGISPRNAEQTF"
     gene            complement(852..1628)
                     /locus_tag="PLI30_06895"
     CDS             complement(852..1628)
                     /locus_tag="PLI30_06895"
                     /inference="COORDINATES: protein motif:HMM:NF026098.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase dimerization
                     domain-containing protein"
                     /protein_id="HOI79316.1"
                     /translation="MNYGFIGFGNLAKAVYQGLKKVNNIQFAYFTRSKKEVSIPFFDC
                     IEELVSFADVIWITVKPQDIKGILEKLQPLNLEGKLIVSPVAGKSIAFIENYLGKEVP
                     IVRIMPNLAMAYQKSVTAFATNQPLNPATDAIFETLTRLGKAVKLNEKGFDLFTSVFG
                     SGPAFILAFIQIFKDKMKEFELPGNVLDELLLELTQGTTLYFAENQSSYSIEELIRNI
                     TSKGGTTQAGLDHFHRHKIGKHFEGVLDAAKNRSEEMSKN"
     gene            complement(1648..2415)
                     /gene="proB"
                     /locus_tag="PLI30_06900"
     CDS             complement(1648..2415)
                     /gene="proB"
                     /locus_tag="PLI30_06900"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: protein motif:HMM:TIGR01027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HOI79317.1"
                     /translation="MRKKLIIKIGTSTLTAGTNRISFAVIESLARQIVELKKEYDIVI
                     VSSGAIATARQFVEINGFHKNVDSKQAMAAIGQTKLMELYDDIFRTFGLNIAQILLTY
                     RDFENETANENTRNTINKLWEVDYIPIVNENDTVSIEEIVLGDNDKLSALVAVITKAD
                     LLILVSDIDGIYTCNPHLHQDAELITSVTDLNSITHCVEEKESTLGTGGMSSKIYAAE
                     VCMRHRVEMWIVNGQRANYIIKALNGGIPFTRFKAKE"
     gene            complement(2417..3604)
                     /locus_tag="PLI30_06905"
     CDS             complement(2417..3604)
                     /locus_tag="PLI30_06905"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: protein motif:HMM:NF001221.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HOI79318.1"
                     /translation="MVISSKNNVLLGLADLLKRNKSEILRKNALDMDSFADMDESMKD
                     RLKVDENKVDGMIRSLREVAEQEDPEGRILYDFTRSDGLRIVNRSVPFGTILIIYESR
                     PDVTIEAAATAFKAGNRILLKGGKESFLTNTYLTELWQQSLSANGFDAYFVEYLNLSR
                     NETQQLIRENTRNVDIIIPRGGDKLINFVTENSSVPVIVSGRGNNFMYVDDNCDTEMA
                     ARLIINGKKRISVCNALDKVLVNENLPELAEKISGFVSQLQGKGIEVWGDKKAASVCS
                     RIQETNDEAVLFEEFMAPKIYFSLVGDMNKAVDMINRYSGGHSAVIVTQDESKADIFM
                     QKVDCAAVYHNASTRFTDGGQFGVGGEIAISTQKLHFRGPLGTQELVTNKWFVYGDGQ
                     IRE"
     gene            complement(3718..5754)
                     /locus_tag="PLI30_06910"
     CDS             complement(3718..5754)
                     /locus_tag="PLI30_06910"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOI79319.1"
                     /translation="MLKKRIRFFLGIIFLPLAIDAQELSPRKEDSTLLLQEVIVNAYQ
                     VNTRWHNVPGSISVLSGREVQAGDGNNFASVLHALPGIYMHSGTYATNRIVIRGVGSR
                     TPYNTNRIKAYLNDIPLTSSDGISSPEDIDLSGIGRIEITKGPASALYGSGLGGNINL
                     FTQDSYRNSFNALLQQGSFNTLKAAIGSSFHHNDLDISGNINHTRSDGYRENNHFRRT
                     SLLSSGSWEKPEHALEYTLLLLDMNAGIPSSIGESLFKTDPKAAAPNWETIGGFKEYR
                     RGLAGITLENRLSGVLNNRLTFFGRWVESYEKRPFNNLTDGSRGGGIRNKLNYHAEKY
                     DAVIGLEWITDTYRWKLDLQDEVINKNSETRNHLNVFAMTYFRPSLKWNVSLGGAVNK
                     IGYELTDKFPGNGNQSGNRGFPVIFSPRLGVNYAPGNRMALYASVGHGFSMPSPEETL
                     LPEGNINKELQPEQGWQYEAGIRLNLLRNTAQVEATVYQINLNNLLVTKRLTEDIFTG
                     INAGKTRHRGVELLWKQQIFHNLNFPGNLHMNANYTFSQNRFIDFTDDNKTYDGNLLP
                     GIPSHLFQTTLVWNPLKQLVLNVRFQYTGEQLIDDANTVTNDAYFLSDGKVSYLFKTT
                     RLGNVELYAGMNNITDTLYSPMLNVNAVAFGNAEPRYYYPGLPRHYYCGVSWSF"
     gene            complement(5764..>7036)
                     /locus_tag="PLI30_06915"
     CDS             complement(5764..>7036)
                     /locus_tag="PLI30_06915"
                     /inference="COORDINATES: protein motif:HMM:NF019607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HOI79320.1"
                     /translation="GDNLEKFAAKQWMEEAGTASVERSIRNGILDIGMPAFAKTFSDR
                     EIKELAGYVKKGIPADRALLKPAVTAEGIVKSEEYNFVIDTVVTGLEVPWGLAFLPNG
                     DLLISERKGTLHTFSNGRLSAPIEGLPPIMAFGQGGLLDLALHPDYDKNSWIYISYSA
                     LDTTSEKRTGSTGVMRARLSENKLTDQEILFIGSPATDRGHHWGCKLAFDGKGYLFFG
                     VGERGQHFDFPQRLDNTNGKIHRIHDDGRIPADNPFVNTPGAIKSIYSYGHRNPQGTV
                     IHPTTGDVWETEHGPMGGDELNLIKPGMNYGWPVISYGINYDGTVLTELTEKEGMEQP
                     VFQWTPSIAPCGMTVVTGDRFKKWENNMLVGSLRFDYIERMVLDGQKVVHTEKLAEGI
                     GRVRNVVMSPDGLVYIGLEEPGMIVRLVPVE"
BASE COUNT         1741 a         1625 c         1544 g         2126 t
ORIGIN      
        1 aaatgtctgt tctgcatttc ggggtgaaat tccgaagcta ggttctttgt ccacacggat
       61 gatcttttgc gtaaacggat tgtatcttgc caaaacactg ttgcgttcac tcttcagtac
      121 aaaagagtcg ttcggaacga gtggattgtc gaaggtaaat tcatccacat caactcccgt
      181 aggttgcgcg tagatcttgt cgatcagttc gggattcaat ccttcaatca cttgttcacc
      241 tctgccgaaa atatcgatat cgataacctt gtcgttgatg taatcttcca ccggaatacc
      301 cagggaacgc gctttcatac gcaggttgat gtctttggtg acaagaatgg ttttcatttt
      361 aggatgtttt tccgttacat ccaaaacggt agataagatg cggttatcgg gagttctttc
      421 gggaaatgct tcaataattt tatcgtgcgt tttcacgcta ttcacaatgt ataactttcc
      481 gcgtcctacg cccagatcag cgccttgttt aaacaaattg tcgtcggtga tcaggtccag
      541 ttcgcgcacg aaagcccgtg cgttgaagtt gatttgttca ttgccttttt tgaatttgtc
      601 aagttcttcc agaacaacaa acggaatgta aatgtcgttt tcttcaaaat tttcgataaa
      661 actgtaatcg tgaagaataa cgtttgtgtc gatgataaaa tttttcttgt tagccatata
      721 ctctattgtt ttgtggtatg tttcattgct gactttggta ataaaaatct ttgttataaa
      781 cgcaaagaaa agatttttgt ttcgatttta aggtataaat atgaaaaaat tcaaaacagt
      841 ttcttaatcg ttcaattttt actcatttct tccgaccgat tctttgcggc atccagcact
      901 ccttcgaaat gcttgccgat cttgtgacgg tgaaaatgat ccagtccagc ctgggtagta
      961 cctcccttgc tggttatgtt ccggatgagt tcctcaatgc tgtagctgga ctgattttcg
     1021 gcaaaataga gtgtggtgcc ttgtgtgagt tcgagcaaaa gttcatcgag aacgtttccc
     1081 ggcagttcaa attccttcat tttatccttg aaaatctgga tgaatgctaa aataaatgcg
     1141 gggccgctcc caaaaaccga tgtgaacagg tcgaatcctt tttcgtttag ttttacggct
     1201 ttgccaagcc gggtaagggt ttcaaaaata gcgtccgtag ctggattgag gggttggttt
     1261 gtggcaaagg ccgtaaccga tttctggtaa gccatcgcca gattaggcat tatgcggaca
     1321 atcggaactt ccttccccaa ataattttcg ataaatgcga tgctctttcc tgcaacgggt
     1381 gaaacgatca gctttccttc aagattcagc ggttgcagct tttcaagaat cccttttata
     1441 tcctgtggct taacggtgat ccagattaca tcggcaaaag aaaccaactc ttcgatacag
     1501 tcgaagaaag gaatgcttac ctctttcttg cttcgggtga agtaagcaaa ttggatgttg
     1561 tttacttttt taagtccctg ataaacagct ttggccaaat tcccgaaacc gataaatccg
     1621 tagttcataa ttggtgtttg atatttctta ttcttttgcc ttaaaccttg taaacggaat
     1681 ccctccgtta agcgccttaa taatgtagtt ggcccgttgt ccgttcacga tccacatttc
     1741 cacacgatgc ctcatgcaaa cttcggctgc gtaaatcttg gatgacattc caccggtacc
     1801 cagtgttgac tctttctctt caacacaatg ggtaatggaa ttgaggtcag ttacgcttgt
     1861 tatcaattct gcatcctgat gcaggtgtgg gttgcaggta tatataccgt cgatatcgga
     1921 aacaagaata agcaagtcgg ctttggtgat aaccgcaacc agtgcggaaa gcttatcgtt
     1981 gtctcccaat acaatctcct caatggagac cgtgtcgttt tcgttcacga taggaatata
     2041 gtctacttcc cagagtttat tgatggtgtt acgggtgttc tcgttagcag tttcgttttc
     2101 gaaatcccgg tatgtaagca gtatttgtgc gatgttcaat ccgaaagtgc ggaaaatgtc
     2161 gtcgtacaat tccatcagtt tggtttggcc gatagctgcc atggcctgtt tagaatccac
     2221 gttcttatga aaaccgttga tctccacgaa ttgtcgtgct gtagcaatgg cacccgaaga
     2281 aacgatcacg atatcgtact ctttctttaa ttcaacaatt tgccgggcta aactttcgat
     2341 aaccgcaaac gaaattcggt tcgttccagc cgtgagcgtg gaggtaccta tttttataat
     2401 cagttttttt ctcatattat tcgcgtatct ggccgtcacc gtaaacaaac catttgttgg
     2461 taaccagttc ttgtgtgcca agagggcccc ggaaatgaag cttttgcgtg ctgatggcaa
     2521 tttctcctcc tacaccgaac tgcccgccat cggtaaaccg ggtggaggca ttgtggtaga
     2581 cggctgcaca atccactttt tgcataaata tgtcggcttt tgactcatcc tgggttacta
     2641 taactgccga atggccaccg gaataacggt ttatcatatc caccgccttg ttcatatcac
     2701 caaccagcga aaaatagatt ttcggtgcca taaattcttc aaaaaggaca gcctcatcgt
     2761 tggtttcctg gatacggcta catactgatg cggctttttt atcgccccaa acctcaattc
     2821 cttttccttg aagttgtgaa acaaaaccgg aaattttctc tgccagttcc ggcaggttct
     2881 cattcaccaa tactttatcg agcgcattgc agacgctgat gcgtttctta ccgttgataa
     2941 taagacgggc agccatttct gtatcgcaat tatcatctac gtacataaaa ttattgcccc
     3001 tgccactcac aattacgggt accgatgaat tttcggtcac aaaattaata agcttatctc
     3061 ctccacgggg aatgatgata tcgacgttgc gggtattttc cctgatcagc tgttgcgttt
     3121 cgttgcgtga taagttcagg tattccacaa aataagcatc aaagccgttt gcagacaatg
     3181 attgttgcca caactccgtc aggtaggtgt ttgtaaggaa ggattctttt cctcctttaa
     3241 gtaagatacg gttacctgcc ttgaatgccg tggctgccgc ttcaatggtt acgtcggggc
     3301 gcgattcata aataataaga atggttccga acggaacgga acggttgact attcttaacc
     3361 cgtctgagcg ggtaaaatcg tacaggatcc taccttcggg atcctcttgt tctgccactt
     3421 cccgaagaga acggatcatt ccgtcaactt tattctcatc tactttcaga cggtctttca
     3481 tcgactcgtc catatcggca aacgaatcca tatccagtgc atttttccgc agaatttccg
     3541 atttatttct tttcaataaa tcagctaagc cgagcaaaac gttatttttt gaggagataa
     3601 ccatttttat gtgaaatagt atggaattaa gctgcaaaga tagcaattta ttctaatttt
     3661 attggtttct atttcaaaat aataaagctg taattaagct tatttcttct tacctgctta
     3721 aaaactccac gaaacaccgc agtagtaatg cctcggaaga ccgggataat agtatcttgg
     3781 ttcggcattg ccgaaagcga cggcattcac gttgagcata ggtgaataaa gggtatcggt
     3841 tatgttgttc atgccagcgt acaactctac atttcccaac cgcgttgttt taaaaagata
     3901 acttactttt ccatccgata agaaataggc gtcgtttgtc acagtattgg catcatctat
     3961 gagttgttca cccgtatatt gaaaccgaac attcaaaacc agctgtttta agggattcca
     4021 aaccaacgtc gtctggaaaa gatgcgacgg aataccgggg agcaggttac cgtcataggt
     4081 cttgttgtcg tcggtaaaat cgataaatcg gttttgagaa aaggtgtaat tggcattcat
     4141 atggagattc cctggaaaat taaggttatg aaaaatctgc tgtttccaca ataactcaac
     4201 cccccggtga cgggtttttc cagcattgat acctgtaaag atgtcttcgg ttaaacgctt
     4261 tgttactaac agattattta agtttatctg gtagaccgtt gcttcaacct gcgcagtgtt
     4321 gcgcagcaaa ttcaaccgga tacccgcttc atattgccaa ccctgttcag gttgaagttc
     4381 tttgttgata ttgccttcag ggagcaatgt ttcttcggga gacggcatcg aaaatccgtg
     4441 tcccacggac gcatacaagg ccatcctatt gccgggtgca taatttactc ccaaccgggg
     4501 agaaaaaata accggaaaac cgcggttccc actttggttg ccgttaccgg ggaacttatc
     4561 cgtcagctca tacccgattt tgttaacggc cccacccagc gaaacattcc atttcaacga
     4621 cggtcgaaaa taggtcatgg caaacacatt caggtggttt cgggtttcgc tgtttttgtt
     4681 gatgacttcg tcctgcagat ccagcttcca tcggtatgtg tcggtaatcc attccaaacc
     4741 gatcacggca tcgtattttt cggcgtgata attcagcttg ttccttattc cgccaccccg
     4801 tgatccatcg gtcaggttgt tgaaaggtcg tttctcgtaa ctttccaccc atcttccgaa
     4861 aaacgtaagt cggttattca aaacaccaga caaccgattt tcaagcgtaa tgcctgcaag
     4921 tccgcggcga tactctttga accccccgat tgtttcccag ttgggggctg ccgcttttgg
     4981 atcggttttg aataatgact cgccaatgga actcggaatt cctgcattca tgtctagcaa
     5041 aagcagcgtg tattccagtg catgttccgg cttttcccaa cttcctgacg agagtaacga
     5101 tgtccgccgg aaatgattgt tttcccggta tccgtcggaa cgggtatggt tgatgtttcc
     5161 ggaaatatcc aggtcgttgt ggtggaagct gcttcctata gctgctttca gggtattgaa
     5221 acttccttgt tgcaacaggg cgttgaagga gtttctgtat gaatcctggg tgaacaggtt
     5281 gatgttgccg ccaagtccgg agccataaag cgcagaggcc ggccctttgg taatttctat
     5341 cctcccgatt ccggacaagt cgatgtcttc gggtgaagaa attccgtcgg atgaagtgag
     5401 gggaatatcg ttcaggtacg ctttgatgcg gttggtgttg taaggtgttc tgcttcccac
     5461 tccccggatc actatacggt tcgtggcgta tgtcccgctg tgcatgtaaa tgccgggaag
     5521 ggcatgcagt actgatgcga aattgttgcc gtcgcctgcc tgtacctctc tccccgacag
     5581 aaccgaaatg ctgccgggga cgttgtgcca tctcgtattc acttgatacg cattcacaat
     5641 cacttcctgg agaagaaggg ttgaatcttc cttccggggc gacagctcct gtgcatcaat
     5701 agccagcggt aagaaaataa tacctaaaaa aaagcgtatt ctttttttca acattttacg
     5761 ggtttattcc accggtacga gccggacaat cattcccggt tcctccaaac cgatataaac
     5821 caacccgtcg ggactcatca ccacgtttct tacccggcct atgccctcgg ccaatttttc
     5881 ggtgtggaca actttttggc cgtctaacac catccgttcg atgtaatcga accggagtga
     5941 accgaccaac atgttgtttt cccatttttt aaaccgatca ccggtgacaa ctgtcatccc
     6001 acagggagcg atagatggtg tccattggaa aaccggctgc tccattcctt ctttttctgt
     6061 cagttcggta agaacggttc cgtcgtagtt gattccgtag gaaatgactg gccagccgta
     6121 attcattccg ggctttatca gattcagttc gtcaccgccc ataggaccgt gttcggtttc
     6181 ccatacatca cctgttgtgg ggtggataac cgtaccctgt ggattgcggt ggccgtagct
     6241 gtagatggat ttaatggccc cgggagtgtt tacgaaagga ttatcggccg ggattcttcc
     6301 atcgtcgtga atgcggtgta tctttccgtt tgtattgtcg agtctctgcg gaaaatcgaa
     6361 atgttgaccg cgttcaccta ccccaaaaaa cagatagccc tttccatcga atgcaagctt
     6421 acatccccag tgatgtccgc gatcggtggc cggtgaccca ataaaaagaa tttcctgatc
     6481 cgtcaattta ttttcgctca aacgagcgcg cattactccg gtggatccgg ttcttttttc
     6541 gctcgtggtg tcgagagctg aataggaaat gtagatccag ctgtttttat cgtaatcggg
     6601 atgaagagcc agatccaaca atccgccttg tccaaaagcc ataatgggcg gtaatccttc
     6661 tatcggtgca gatagtcttc cgttggagaa ggtgtgcagt gttccttttc tttccgaaat
     6721 caataaatct ccattaggta gaaaagccaa accccaggga acttccaatc cggtaaccac
     6781 ggtgtcgatc acgaaattgt attcttccga tttaacaatt ccctcagccg tcacagccgg
     6841 tttgagtagc gctctgtcgg cggggattcc ttttttaacg taaccagcca gctcttttat
     6901 ttccctgtcg gaaaaagttt ttgcaaaagc gggcatcccg atatccaata tcccgtttct
     6961 gatgcttcgt tctaccgatg cggttcccgc ttcctccatc cattgtttgg cggcaaattt
     7021 ttctaaattg tcgccg
//
LOCUS       DAOSDR010000145         7028 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrimonas sp. isolate AMR_MDS_1826
            NODE_3914_length_7028_cov_3.931306, whole genome shotgun sequence.
ACCESSION   DAOSDR010000145 DAOSDR010000000
VERSION     DAOSDR010000145.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863355
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrimonas sp. (wastewater metagenome)
  ORGANISM  Petrimonas sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Dysgonomonadaceae; Petrimonas.
REFERENCE   1  (bases 1 to 7028)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7028)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:10:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,720
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 35
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7028
                     /organism="Petrimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3914_length_7028_cov_3.931306"
                     /isolate="AMR_MDS_1826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2023866"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1213)
                     /locus_tag="PLI30_06920"
     CDS             complement(<1..1213)
                     /locus_tag="PLI30_06920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019541078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HOI79321.1"
                     /translation="MSRFTFILFACFVVFSTGYADENNPPQVNDSIRLEEVIVTGTLP
                     KVNLRNVPMSISIVTERQIQTRLEPSLLPLLTEEVPGLYITQRGVMGYGVAAGAAGGM
                     SIRGIGGSPTSGVLVLIDGHPQYMGLMGHPLADSYQSLMTERVEVVRGPASVLYGSNA
                     MGGVINIITKKQKQNGLHNSAQLMYGTYNTFSAEASSGWKKEKLHINGNIGYNRSDGH
                     RENMDFEQINGYGKIGYDLTGNWSSFVDLNLSNAQSSNPGTVAAPITDNDADITRGMT
                     SFSLENEYERTSGAVKFFYNFGTHKINDGYTEGQQPKPFRFHSDDRMLGLSVYQSYTF
                     FEGNKTTAGVDFQRFGGKAWNKFPDESKNVQLADEHLNNVAGYVNIQQSLLENRLTLN
                     TGIRLDNHEKNG"
     gene            complement(1210..1608)
                     /locus_tag="PLI30_06925"
     CDS             complement(1210..1608)
                     /locus_tag="PLI30_06925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006799629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase"
                     /protein_id="HOI79322.1"
                     /translation="MNTKSFEAVMQRFREIEFHETFDMIVAIANGGIIPAAILNQRLN
                     TEIQLLKINLRDPCQKPKYDSPRLISPVDFDFRDKTILLVEDRIKTGATVKFAIDLLQ
                     GARQIKTFAVNGNADYALYDEDCFKFPWIV"
     gene            complement(1618..2127)
                     /locus_tag="PLI30_06930"
     CDS             complement(1618..2127)
                     /locus_tag="PLI30_06930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009127465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HOI79323.1"
                     /translation="MATTAKLYSFSLSNTKTYLFATCFVLGNLLLPQLAHLVPQGGLI
                     FLPIYFFTLIAAYKYGIHVGLLTAILSPLANNLLFGMPPSAVLPAIIIKSVILAIAAS
                     MVAKHSGKVSFGGILLAIVAYQVIGTGIEWAMTQHFFTAVQDFRIGLPGMLIQLTAGY
                     FILKALAKV"
     gene            complement(2291..3403)
                     /locus_tag="PLI30_06935"
     CDS             complement(2291..3403)
                     /locus_tag="PLI30_06935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007216865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HOI79324.1"
                     /translation="MMQAQENLGFGQRKEIISPEIHADNSVTFRLLAPLADSVSVTGD
                     FAQGHNKMTKDADGVWSFTTRTLPSELYTYAFTANGLQMNDPNNVHYRRDVASTLNVL
                     LVGGGQADIYKVNNVPHGTVAKRWYESPGNKTDRRITIYTPPGYESGNTSYPVLYLLH
                     GMGGDEEAWPTLGRATQIIDNLIAEGKAKPMIIVMPNGNVAQEAAPGESSEGFYKPTF
                     RLPNTMDGKYEETFIDIINFVDKNYRTVRNKSGRAIAGLSMGGFHSFHISRYYPNSFD
                     YVGLFSAAIMPNENAKSSVYKNIEDTLLKQKQNGYKLYWIGIGKDDFLYQANTDFRKK
                     LDGINFKYAYRESEGGHTWRNWRVYLSEFLPLLFNQ"
     gene            complement(3624..7010)
                     /locus_tag="PLI30_06940"
     CDS             complement(3624..7010)
                     /locus_tag="PLI30_06940"
                     /inference="COORDINATES: protein motif:HMM:NF018178.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1080 domain-containing protein"
                     /protein_id="HOI79325.1"
                     /translation="MKKYLSILTAILLATGALMAQLPQGRTSATIIADALAQLPADNQ
                     TQYNRTMTDLVSTGEQGLLDLIGRMNPPGNRSNEALDYAISGWTNFVANDDAKRALAA
                     NAFGKALNQSLDNEVKAFVIRQLGQIGTDNNVETLSAFLKDSHLSAPAVQALASIGSE
                     KANQAIVAALSGNAPDAVKFNIVNALTQTGYSQAEPALLSTLSQNPSAELSNALLTAL
                     GSLGSRNALIPLKNAAQKVNYSYQLNNNATASYLSLLKRLTHTDVKLVEKEANGLLNF
                     AIKQNRSDLKVAAAGLLLAIPSTDKNKLLNSALKDGDIAYLARLLNAYPFNNDRKAVE
                     RIMKGLSPKASAEKQTAIIYWLGDKKVANTANMLANFATSGNKMVQKAAISSLAKIGN
                     EQAMLVLAGLLKSQDDETVLLAKDALSTYKGDISYTLASVFNESGDVGKKAILQLIAN
                     RKMESQYNLVYNQMFTGNENVKTEAANTLQYVSTDKNLPDLFTLLEQSDAANVPALQQ
                     AVNAALSYLPANEQMKLVSDRMNKSVNKHLYYTALANSGSQKAMEMITKAYNTETGAN
                     KNAAFDALTNWKSFNSIYPMLDIARNSKNKNELSKVTDAIVATINKSNETGAIKYLYL
                     REVMQFAQTEKQKNDILRLLGNTGQYQAMLFVAPYMDNVALSENAALAAMNIATNNPA
                     FAGVVTTGILQKVSKTLKNPDAGYQRESIKKYLDENPQDGGFVSIFNGKNLDGWKGLV
                     ENPIKRAKMTPKELAAAQVKADAAAKTGWVIENGELLFTGKGDNLCTNKQYGDFEMLV
                     DWKLYPGPEPDAGIYLRGTPQVQIWDTARVNVGAQVGSGGLYNNQQNPSKPLKVADQK
                     VGEWNTFRIKMIGERVSVWLNDELVTDNVVLENYWNRSQPIFPTEQIELQAHGSKVAY
                     RDIFIKEIERPEPFQLPADEKKEGFRVLFDGTNLNEWTGNKKDYVVESGNIVLYPSQN
                     FGGNLYTKEQFDNFIFRFEFMLTPGANNGLGIRAPLEGDAAYGGMELQILDNDAPVYK
                     NLQIYQYHGSVYGVIPAKRGYLKPVGEWNYQEVIADGDRIKVILNGTTILDGNIREAS
                     KNGTIDKRDHPGLLNKTGHIGFLGHGSLVKFRNIRIKPLK"
BASE COUNT         1702 a         1568 c         1610 g         2148 t
ORIGIN      
        1 acccgttctt ttcgtggttg tcgagtctta tcccggtatt caatgtgagt ctgttttcca
       61 gtaagctttg ctggatattg acgtatccgg caacgttgtt caggtgctca tcggctaact
      121 gtacattctt cgactcgtcg gggaatttgt tccacgcttt ccctccgaaa cgctgaaaat
      181 caactcccgc tgtggtcttg ttgccctcga aaaaggtgta agactgatag accgaaaggc
      241 ccagcatccg gtcgtctgaa tggaaacgaa acggtttggg ctgttgtcct tctgtgtacc
      301 cgtcgttgat tttatgcgtc ccgaaattgt aaaagaattt tactgctcct gaagttcttt
      361 cgtattcatt ttcaagagaa aatgaggtca tcccgcgtgt aatatcggca tcgttatcgg
      421 ttatcggagc agcgacagtc cccggattgg acgattgggc gttcgaaaga ttcaaatcca
      481 caaaactgct ccaatttccg gtaagatcgt atcctatttt tccgtaaccg tttatctgct
      541 caaaatccat gttttcccga tgtccgtcgg aacggttata cccaatgttc ccgttgatat
      601 gcagcttctc tttcttccat ccgctggatg cttcagcgct gaaagtattg taggtgccgt
      661 acattaattg agccgaattg tgcaagccgt tctgcttctg ctttttggta atgatattga
      721 ttactccacc cattgcattg gatccgtaaa gcactgacgc agggccacga accacttcca
      781 cccgttcggt catcagggat tggtagctgt cggccagagg gtgtcccatc agacccatat
      841 attgtggatg tccgtcgatg agtaccagta ctcccgaagt aggggaaccg ccaattccgc
      901 ggatgctcat tcctccggca gcaccggcag ctacgccgta tcccattact ccacgttgtg
      961 tgatgtatag tccgggaact tcctcggtaa gcagcggcag aagcgaaggt tccagtcggg
     1021 tttgaatttg ccgttccgtg acgatagata tgctcatcgg cacgtttctc aggttcacct
     1081 tcggtagcgt accggtgacg atcacctctt ccaggcggat agaatcgttg acctgcggtg
     1141 gattgttctc atcagcataa ccggttgaga aaacaacgaa acaggcaaaa aggataaatg
     1201 taaatctact catacgatcc agggaaactt aaaacaatct tcgtcgtaga gtgcataatc
     1261 agcatttccg tttactgcga aagtttttat ttgtctggca ccttgtaaaa gatcgatggc
     1321 gaatttaaca gtcgctcctg tttttatgcg atcttccacc agcagaatgg ttttgtccct
     1381 aaaatcgaag tcgacaggcg aaatcaacct ggggctatcg tattttggtt tttggcaggg
     1441 atcacgaaga ttaattttca ataattgaat ttcagtattc aaccgctgat taaggattgc
     1501 ggccgggata atcccgccgt tggcaatggc cactatcata tcaaaagtct cgtgaaattc
     1561 aatctcacga aacctttgca taacagcttc aaaagacttt gtattcatta aataacctta
     1621 cactttagcc aaggctttca gtatgaaata tccggctgtt agttgaatga gcattccggg
     1681 aaggccgatg cggaaatcct gaacggctgt gaaaaagtgt tgggtcatcg cccattcaat
     1741 tcctgtaccg atcacttgat aggcaacgat agccaaaaga atacccccga aggatacttt
     1801 tccggaatgt tttgccacca tggaggcggc aatagccaaa ataaccgatt tgatgatgat
     1861 tgccggcaac acggcggaag gaggcatacc gaacaacaag ttgttagcta aaggcgatag
     1921 tattgccgtc aacaacccaa catgaattcc gtatttgtac gctgcgatga gcgtaaagaa
     1981 atagatggga agaaaaatca gtcctccctg gggcactaaa tgcgccagtt gcggcaataa
     2041 taagttgccc aacacaaaac atgtagcgaa caagtaggtt tttgtgttgc tcaggctgaa
     2101 tgaataaagt tttgctgttg ttgccataat ctgatggttt tattatttgt tattgtttta
     2161 gttttgaatt cctgctctaa aatgcaacat aggtgacaaa aataattata atcttacgaa
     2221 taacccgctt tttagccatt ttttttatta gaaaaaataa aattgcaatc tttgcttggc
     2281 aatcagtgag ttattggttg aatagcaacg gtaagaattc cgataagtaa actctccagt
     2341 ttcgccaggt gtgtccgcct tccgattccc ggtaggcata tttgaaattt attccgtcga
     2401 gttttttccg aaagtcagta tttgcctgat acaaaaaatc gtctttcccg atacctatcc
     2461 agtaaagttt ataaccgttc tgtttctgtt ttaacagtgt atcctctata tttttataca
     2521 cgctggattt cgcattttcg ttaggcataa tagctgcaga aaataaaccc acgtaatcga
     2581 aactgttggg ataataccgt gaaatatgaa aagaatgaaa acctcccatg gaaagtccgg
     2641 caatagcacg cccggatttg ttccggacag tgcggtaatt tttatctacg aagttgatga
     2701 tatcgataaa tgtctcttcg tattttccgt ccatcgtgtt tggtaacctg aaagttggtt
     2761 tgtaaaaccc ttcgctcgat tcacccggcg cagcctcctg ggctacgttt ccgttcggca
     2821 taacgataat catcggtttg gccttgcctt cggcaatcag gttgtctata atctgggtcg
     2881 cgcgtccaag cgttggccac gcttcttcat cgccacccat gccatgaagc agatacaaaa
     2941 caggatagga tgtgttaccc gattcataac cgggaggagt gtaaatggtt attcgtctgt
     3001 cagtcttgtt gcccggagat tcataccatc tcttggctac cgtgccgtga gggacattat
     3061 tcactttata tatatcggct tgtccaccac caaccagcag aacatttagt gtgcttgcta
     3121 catcgcgacg gtaatgcacg ttattgggat cgttcatttg taacccgttt gccgtaaagg
     3181 catacgtgta taattccgaa ggaagcgtac gggttgtaaa tgaccaaacg ccatcggcgt
     3241 cttttgtcat tttattgtgg ccttgtgcaa aatcacccgt taccgacaca ctgtcggcca
     3301 agggagcgag taaacgaaac gttacggaat tgtcggcgtg gatttcaggg gaaataattt
     3361 cctttcgttg tccgaagcca aggttttcct gtgcttgcat catgatgctg gtggagaata
     3421 cgagcagagt aaaaaagatt tttttgtaca taggttttca ggtttacgat tgagtgtata
     3481 tttgcaattg tcagatctgt attgcaaata tagtttttat tttgaaatat cgggtatcga
     3541 gagggttaat atcgataaac agcatctttg tgccgatgga ccaaaaaaaa gagacgcatt
     3601 caatgcgtct cttggataaa ttgttacttc agtggtttta tccggatgtt tcggaatttt
     3661 accaaggagc cgtgccccag gaaaccaata tgtccggttt tattcaataa cccgggatga
     3721 tcccgtttat caatcgtccc gtttttggag gcttcgcgga tgtttccgtc caggatagtt
     3781 gtaccgttaa gaataacttt gatgcgatcg ccatcggcaa ttacttcctg atagttccac
     3841 tcgcctacag gcttcaggta accgcgttta gcaggaataa cgccataaac cgaaccgtga
     3901 tactggtaga tttggagatt tttgtagacc ggggcatcgt tatcaagaat ctgtaattcc
     3961 attcctccat atgcggcatc gccttctagc ggcgcgcgga tgcctaatcc gttgttggca
     4021 cccggtgtga gcataaattc gaaacgaaat ataaaattat cgaattgttc tttggtgtaa
     4081 aggttgccgc caaaattttg gctgggatac agcacgatgt ttccactttc caccacgtag
     4141 tcttttttgt ttcctgtcca ttcgttgagg ttagtaccgt cgaagagtac ccgaaaacct
     4201 tctttctttt cgtcagcagg aagctggaaa ggttcggggc gttcaatttc tttaataaat
     4261 atatcgcgat aagccacctt gctgccgtgt gcctgcaact caatctgttc cgtcgggaag
     4321 atgggctgac tcctgttcca gtagttttcc aacactacat tgtcagtaac cagttcatcg
     4381 ttaagccaca ccgacaccct ttcaccaatc atcttgattc ggaatgtatt ccattcacct
     4441 actttctggt cagcaacttt tagcggtttg ctcggattct gctggttgtt gtacaatcca
     4501 cctgatccaa cctgtgctcc tacatttaca cgggcggtgt cccagatctg aacctgtggc
     4561 gtgccgcgaa ggtagatccc tgcatcaggt tccggcccgg gataaagctt ccagtcaacc
     4621 agcatttcga aatctccgta ttgtttgttg gtacagaggt tgtccccttt tccggtgaag
     4681 agcagttcac cgttttcgat gacccagccc gtttttgctg ctgcgtcggc ttttacctgg
     4741 gctgcagcga gttcttttgg cgtcattttc gcgcgtttga tgggattctc taccaagcct
     4801 ttccatccgt ccaggttttt accgttgaag atggacacaa aacctccgtc ttgaggattt
     4861 tcgtccaggt atttttttat ggactcacgt tgatagccag catctggatt tttcaatgtt
     4921 ttactcactt tctggagtat tccggtggtt acaacacccg caaaagccgg attatttgtg
     4981 gcgatgttca tggcagccaa tgcggcattt tcgctcaatg ccacattatc catatacggg
     5041 gctacgaaaa gcatagcctg atattgtccg gtgtttccca acaggcggag aatgtcgttt
     5101 ttctgtttct cggtctgtgc aaactgcatc acttcacgga gatacaggta ttttatggct
     5161 cctgtttcat ttgatttatt gatggtagca acgattgcat ccgttacttt gctcaattca
     5221 tttttgttct tactgtttct ggctatatcc agcatcgggt aaatggagtt gaacgatttc
     5281 caattggtca gtgcatcaaa agcagcattt ttattggccc cggtttcggt attgtaggcc
     5341 ttggtaatca tctccattgc tttttgagac ccgctgtttg ccagtgcagt atagtaaagg
     5401 tgcttgttta cagacttgtt catccggtcc gataccagct tcatttgttc gttggccgga
     5461 agatacgaca gggctgcgtt tacagcttgt tgaagagcgg gaacattggc agcatccgat
     5521 tgttcgagca atgtgaacaa gtcgggcagg tttttatccg tagaaacgta ttgaagcgta
     5581 ttggctgctt ctgtttttac gttttcgtta ccagtgaaca tttggttata aaccaggtta
     5641 tactgacttt ccattttccg gttggcaata agctgtagga tagctttttt cccgacatcc
     5701 ccgctttcgt tgaaaaccga tgcaagtgta taggagatat cacccttgta tgttgataaa
     5761 gcatctttgg cgagcaacac cgtttcatcg tcctggcttt tcaatagtcc ggctaataca
     5821 agcatcgctt gttcgtttcc aatttttgcc aaggaagaaa tggccgcttt ctgtaccatt
     5881 ttattaccgg atgtagcgaa attggcaagc atattagctg tattcgctac tttcttatcg
     5941 cccaaccagt aaatgatggc ggtttgtttc tctgcagaag cttttggact tagtcccttc
     6001 ataatccttt caaccgcttt tcggtcgttg ttgaaaggat aggcgttaag caaacgggcc
     6061 aggtaagcga tatccccgtc tttcagtgca ctgttgagga gtttattttt gtccgtcgaa
     6121 ggaattgcca gtaaaagccc ggctgcagcc actttcagat cggaacggtt ttgctttatg
     6181 gcaaagttga gcaacccatt tgcctccttt tccaccaact taacgtcggt gtgagttaat
     6241 ctttttaaaa gagaaaggta ggatgccgta gcattgttgt taagctgata ggaataattg
     6301 actttttgtg cggcattttt cagtgggata agggcattgc ggcttcccag gcttcctaaa
     6361 gccgtaagca atgcgttgct gagttctgct gagggatttt gtgacagtgt tgagagcagt
     6421 gcgggttcgg cttggctgta tccggtctga gttagtgcgt tcacaatatt gaactttaca
     6481 gcatccgggg cgttacccga aagtgctgca acgatggctt gatttgcctt ctcacttccg
     6541 atggaagcca atgcctgtac agccggagct gaaaggtgcg aatccttcag aaaagcagat
     6601 aaagtttcca cattgttgtc agttcctatt tgtccaagct ggcggataac aaaagctttt
     6661 acttcattat ccagcgattg gttcaatgct tttccgaaag catttgccgc aagggcccgt
     6721 ttggcatcat cgtttgcaac aaagtttgtc catccgctga tggcatagtc caatgcttcg
     6781 ttgctccggt tgcccggagg gttcattcgt ccgatcaagt ccagtaaacc ctgttctccc
     6841 gtggaaacca gatcggtcat cgtccggtta tattgagttt gattatcggc cggaagttgt
     6901 gccagtgcat ccgcaataat ggtggcactt gttctgccct gcggcagttg agccattaat
     6961 gcacccgttg cgagcagaat ggctgttaaa atggataggt attttttcat tgttgtttaa
     7021 tttaattg
//
LOCUS       DAOSDR010000146         7004 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrimonas sp. isolate AMR_MDS_1826
            NODE_3930_length_7004_cov_3.485681, whole genome shotgun sequence.
ACCESSION   DAOSDR010000146 DAOSDR010000000
VERSION     DAOSDR010000146.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863355
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrimonas sp. (wastewater metagenome)
  ORGANISM  Petrimonas sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Dysgonomonadaceae; Petrimonas.
REFERENCE   1  (bases 1 to 7004)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7004)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:10:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,720
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 35
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7004
                     /organism="Petrimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3930_length_7004_cov_3.485681"
                     /isolate="AMR_MDS_1826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2023866"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..429)
                     /locus_tag="PLI30_06945"
     CDS             complement(<1..429)
                     /locus_tag="PLI30_06945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI79326.1"
                     /translation="MQKILEHSSQRARIIYLLLFSLMGSFVAGAILFMAGISLPSAEG
                     SIWYYRFSIILQDIFVMFLPAYTLCLWIADKPMQMMGVRKTRKMSQGLLYGFLIFGVS
                     YPAIAVIAQWNEQIVLPDSLTGVENWMRQMEDAAKDVTDLF"
     gene            643..2016
                     /gene="tig"
                     /locus_tag="PLI30_06950"
     CDS             643..2016
                     /gene="tig"
                     /locus_tag="PLI30_06950"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006798749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HOI79327.1"
                     /translation="MKVTLNKTDNVNGIIAIELEKPDYQENVDKSLNQFRQKANIPGF
                     RQGKVPKGIIQKMYGKSILAEEINKLVSNELYKYIKENELNILGEPLPNEEEQKTIDF
                     DKDENFEFKFDVALAPEFELPLNRRDTLIYYNVKLEDDLLDKQYDAYKQNYGSYKKVE
                     GETVETDLIKGKISEIDNGEPKENLIEIENGMIMPSYIKDEETKKKFVGAKVGDTVVF
                     NPKTAYDNNAAEIASLLQIAKENVADVDSDFSFDIQEVTRYEEAAMEQELFDKVLGEG
                     VVSSEEEFKVKVTELLNNQFKPAADNLFMKSARELILKKLKDVEFPDVFLKRWLLVAN
                     EKNTAETIENDYPKIAEDLKYHLSKEKIVKEQDIKIENSDLETFAAEVARAQFAQYGM
                     SNVPADVLENYVKRMLGDQNTVRNMYDQLVENKVMEWLKQTVKVNEKEIPSKDFEKLL
                     SEDKEEK"
     gene            2155..2829
                     /gene="clpP"
                     /locus_tag="PLI30_06955"
     CDS             2155..2829
                     /gene="clpP"
                     /locus_tag="PLI30_06955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019540791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HOI79328.1"
                     /translation="MQNDFRKYAVKHLGMSSARLDGYTKIISAGGGYISPTIIEERQL
                     NVAQMDVFSRLMMDRIIFLGTQIDDYTANVIQAQLLYLDSADQGKDISIYINSPGGSV
                     YAGYGIYDTMQFISSNVTTICTGMAASMAAVLLVAGTSKKRFALTHSRVMIHQPLGGV
                     QGQASDIEITAREIAKVKQELNAIISKHTGRPIEEVSRDSDRDFWMSASEAKEYGMVD
                     DILIKK"
     gene            2879..4120
                     /gene="clpX"
                     /locus_tag="PLI30_06960"
     CDS             2879..4120
                     /gene="clpX"
                     /locus_tag="PLI30_06960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009017965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HOI79329.1"
                     /translation="MAKKANSSNHCSFCGRSENDVNLLISGISGYICDMCSEQAHEIV
                     NETFKSSGKSAPDISLNSLPKPKQIKEFLDQYVIGQDSAKRFLSVSVYNHYKRILQKT
                     VKDDVEIEKSNIIMVGATGTGKTLLARTIAKLLRVPFTIVDATVLTEAGYVGEDIESI
                     LTRLLQVADYDVAAAERGIVFIDEIDKIARKSDNPSITRDVSGEGVQQGLLKLLEGSV
                     VNVPPQGGRKHPEQRMIAVNTKNILFVCGGAFDGIEKKIANRLNTRVVGYAASGRTAD
                     IDRNNLLQYITPMDLKAFGLIPEIIGRLPILTYLEPLDRDALLRILTEPKNSIIKQYE
                     KLFSMDGVTLTLDKDVYEYIVDKAIEFKLGARGLRSIVEAIMIDAMFSLPSEDKKKLH
                     VTREYAVKHFEKSDFRHLRVA"
     gene            4344..6545
                     /gene="recQ"
                     /locus_tag="PLI30_06965"
     CDS             4344..6545
                     /gene="recQ"
                     /locus_tag="PLI30_06965"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008156887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HOI79330.1"
                     /translation="MEKQKTLYEELKKYFGFDTFKGNQKAIIESVLSGKDTFVLMPTG
                     GGKSLCYQLPALLSKGTAIIISPLIALMKNQVDAMRNFSEEDGVAHFLNSSLNKQEIE
                     KVKQDIVSGKTKLLYVAPESLTKMENIDFLQNVPISFYAVDEAHCISEWGHDFRPEYR
                     RIKPIINEIGPRPVVALTATATPKVQHDIQKTLGMLDAAVFKSSFNRSNLYYEVRKKT
                     DKVDKEIIKYILSQGIKSGIVYCLSRKKVDDFAQILQANDIRALPYHAGMDAATRSAN
                     QDAFLMEQADVIVATIAFGMGIDKPDVRYVIHYDIPKSLEGYYQETGRSGRDGGEGKC
                     IAFYSSKDLQKLERFMQGKPVSEQEIGKQLLLETAAYAETSVCRRKMLLHYFGEDYNE
                     PNCGNCDNCLNPKIKVEAKELLVTVLEAISVLKEKHKADYLINFLLGKETSEIETFEH
                     NELEEFGSGSDEEESTWETVIRQALLDNYLKKDIENYGILKITKKGKEFLKKPVSFKI
                     TKEEDEDDADIEELESEEGVIASGGGNSGAADPALFSMMKDLRKKISKKLNVPPYVIF
                     QPASLEAMATSYPITLEELQNIPGVGAGKAKRYGQEFIELIKKHVEENEIIRPEDLRV
                     KTVANKSKLKVSIVQAIDRKVALDDLAESKGIDFNEMLSEVEAIVYSGTKININYFLE
                     EVMDTDTVREIYEYFREAETDNLDKAMDELSDYSETEIRLVRIKFFSEMAN"
     gene            6615..>7004
                     /locus_tag="PLI30_06970"
     CDS             6615..>7004
                     /locus_tag="PLI30_06970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019540788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase
                     N-terminal domain-containing protein"
                     /protein_id="HOI79331.1"
                     /translation="MTYLQDINSPADLKKFNVEQLKEVCKDLREFIIEQLSHNPGHFA
                     SSLGTVELTVALHYLYNTPYDKLVWDVGHQAYGHKILTGRRDVFHTNRKLGGLSGFTN
                     PQESIYDTFVAGHASTSVSAAVGIAMAR"
BASE COUNT         2268 a         1458 c         1544 g         1734 t
ORIGIN      
        1 gaagagatcg gtaacatcct tggcggcgtc ctccatctgc cgcatccaat tctccactcc
       61 cgtcaatgag tcaggaagca caatctgttc attccactga gcgataaccg ctattgcggg
      121 ataggaaact ccaaaaatta aaaaaccgta cagaagacct tgcgacattt ttcgggtttt
      181 ccttaccccc atcatctgca tcggcttatc cgcaatccat agacaaagag tgtaagcggg
      241 cagaaacatc acaaaaatat cctgcaagat aatgctgaac cgataatacc agatgcttcc
      301 ctctgcggat ggcagtgaaa ttccggccat gaacaagatg gctccggcca caaatgagcc
      361 catcagggag aacaaaagca ggtagataat tcttgcacgc tgtgagctat gttcgagtat
      421 tttttgcatg ttttgtaatt tcgagctgca aagatagcta aaatagggtg aatgcgaagg
      481 gcttattttg cattgtttaa ataattaact ctcaaatatt ttttgcagtt atcaatataa
      541 agcgtatttt tgcatccaat tttaaaaaat cggttttact atttaaatcg attgattttc
      601 aaactggaac aaaacttata aagcgtaaaa taaacagttc aaatgaaggt aacactcaac
      661 aaaacagaca atgtaaacgg catcatcgcc atagaattag aaaaaccgga ttatcaggaa
      721 aacgttgaca aatcgctgaa tcaattccgt caaaaagcca atatccctgg ttttagacaa
      781 ggaaaagttc ccaaaggaat tattcagaaa atgtatggaa aatctattct tgccgaagag
      841 atcaacaagt tagtaagcaa cgaattatac aaatacatca aagaaaacga gctgaatatc
      901 ctgggtgagc cgctgccaaa cgaagaagaa caaaaaacca tcgatttcga caaggatgaa
      961 aatttcgaat ttaagtttga tgttgctttg gctcctgaat ttgaacttcc actcaacaga
     1021 agagacacgc tcatttatta caacgtcaag ctggaagatg atttgcttga taaacaatac
     1081 gatgcctaca aacaaaatta cggctcctat aaaaaagtgg aaggcgaaac ggtcgaaacc
     1141 gatcttatca aaggaaagat atcggaaatc gataacggcg aacctaaaga gaacctgatt
     1201 gaaatcgaaa acggcatgat catgccatcg tacatcaagg atgaagagac aaaaaagaag
     1261 tttgtgggtg ccaaggtggg cgatactgtc gtcttcaacc ccaaaacagc ctatgacaac
     1321 aacgctgcgg aaatagcatc gttgttgcag atcgccaaag aaaacgtagc ggatgtcgac
     1381 tccgactttt cttttgatat ccaggaagtt acccggtacg aagaagcagc tatggagcag
     1441 gaattgtttg ataaagtgtt gggcgaaggt gtggtttcat cggaagagga attcaaggta
     1501 aaagtcacgg aattactgaa caaccagttc aaaccggcag cagataacct gtttatgaaa
     1561 tcggcacgtg aactgatcct gaaaaagttg aaagacgttg aatttcccga tgttttcctg
     1621 aaacggtggt tattggttgc caacgaaaaa aacacagcgg aaacgatcga aaacgactac
     1681 ccgaaaatcg ctgaagattt aaaatatcac ctgtcaaaag agaaaattgt aaaggaacag
     1741 gacattaaaa tcgaaaacag cgacctggaa acctttgcag ccgaggttgc cagagcacaa
     1801 tttgcgcaat acgggatgag caatgttccc gccgacgtgt tggaaaacta tgttaaacgg
     1861 atgctgggtg accagaatac ggtcagaaac atgtacgacc agctggttga aaacaaagtg
     1921 atggaatggc tcaaacaaac cgttaaagtc aacgagaagg aaattccatc aaaagatttc
     1981 gagaagttac tgtcggagga caaagaagaa aaataaaatc ttctgcgtaa acaaggagat
     2041 tatctcctaa aaataaatat ctttgtttca cggaaaacga tattccgatg gttgattgtc
     2101 taaatcacac acattgcatc aggagtaaaa ccggtgataa aaaaacaaaa aattatgcaa
     2161 aacgatttca gaaaatatgc cgtcaagcat cttgggatga gcagtgcccg attggacggc
     2221 tatacaaaaa taataagtgc aggtggcgga tacatctctc cgaccattat cgaagaacgt
     2281 cagttgaacg ttgcccaaat ggatgtgttt tcacgtctga tgatggaccg gatcatcttt
     2341 ctcggcactc agatcgacga ttacaccgcc aatgtcatac aggcgcagct gctttatctc
     2401 gactctgctg atcagggaaa agacatctcc atttacatca attctcccgg tggatccgtt
     2461 tatgccggtt atggaatata tgacaccatg cagttcatct ccagcaacgt aaccaccatc
     2521 tgtaccggga tggcagcatc tatggccgcc gttctgctgg ttgccggaac atccaagaaa
     2581 cgctttgcgc tcacgcactc acgtgtaatg attcaccaac cgctgggcgg cgttcaggga
     2641 caggcatctg acattgagat taccgctcgc gaaattgcca aggtaaaaca ggaattaaat
     2701 gccatcattt caaaacatac gggaagaccc atcgaggaag tgtctcgcga ttcggaccgt
     2761 gatttctgga tgagtgcttc agaagccaaa gaatatggaa tggtggacga tatattaatt
     2821 aagaaataag aagttagaaa acagatttct gaaatctgat atctaaaagt ctaataaaat
     2881 ggcaaaaaaa gcaaacagca gcaatcattg cagtttctgc ggaagaagtg aaaacgatgt
     2941 gaacctgctt atctcgggaa tatcgggtta tatctgcgat atgtgttccg agcaggcaca
     3001 cgaaatcgta aacgaaacgt tcaaaagcag tggaaaatcg gctcccgaca tttcactcaa
     3061 ttcacttccc aagccgaaac agatcaagga atttcttgac cagtacgtta ttggccaaga
     3121 cagtgccaaa cgctttctgt ctgtttcagt ttacaaccat tataagcgaa tcctgcaaaa
     3181 gacggtgaaa gacgatgtgg aaatcgaaaa atcaaacatc attatggtcg gtgccaccgg
     3241 aacaggaaaa actttgttgg ctaggacgat cgccaaactg ctgcgtgttc cgtttaccat
     3301 cgtcgacgct actgttctta ctgaagccgg atacgtggga gaagacattg aaagtattct
     3361 cacccggttg ctgcaggttg ccgattatga tgtcgccgca gcagagcgtg gtatcgtttt
     3421 tatcgatgaa atagataaaa ttgcccgtaa aagcgataat ccgtccatca cccgcgatgt
     3481 cagcggcgaa ggtgttcaac aaggattgtt gaagttgctg gaaggatcag tcgtaaacgt
     3541 tcctccgcaa ggcggaagaa agcatcccga acagcgcatg attgcagtga ataccaaaaa
     3601 tatattgttt gtttgcgggg gcgctttcga cgggattgag aaaaaaatag ccaacaggct
     3661 gaatacgcgc gtagtaggtt atgctgccag cggaagaacc gccgatatag accgtaacaa
     3721 cctgctccag tacatcaccc cgatggattt aaaagctttt ggcttaatac ccgaaattat
     3781 cggacgcctg cctatactta cctatctgga acccttagac agggatgctt tgctgagaat
     3841 cctcaccgag cccaaaaact ccatcatcaa acagtacgaa aaactcttct ccatggacgg
     3901 tgttacactc accctggata aggatgttta cgagtacatt gttgacaaag ccatcgaatt
     3961 caaactcggt gcccgtgggt tacgttccat tgtggaagcc attatgatag acgccatgtt
     4021 cagtttgcct tccgaagaca agaagaaact acacgtaact cgcgaatatg cggtgaagca
     4081 tttcgaaaaa tcggatttcc ggcacctgag agtagcttaa attgcctttt ctcttttctt
     4141 ttttccttcc tttttcagga agtggtttgt tgtgtcttta tttctcccga aagatgaata
     4201 tcagaatttg tttattcggt ttttattctt aaatttagtg tttatttttg aattggaatt
     4261 gtttttaaat ataaaatact ttcttacatt tgcattattc catttgcaca aaaatgtttt
     4321 aagtttgtag cggaattgct cttatggaaa aacaaaagac tctatacgaa gaattaaaga
     4381 aatatttcgg gttcgacacg tttaaaggaa atcaaaaagc cattatcgaa agtgtcttgt
     4441 ccgggaagga tacttttgta ttaatgccta ccgggggcgg taaatcgctt tgttaccagc
     4501 tgccggcatt gctttccaaa ggtacggcaa ttatcatttc gccacttatc gcactgatga
     4561 aaaatcaggt ggatgccatg cggaatttta gcgaagaaga cggcgttgct cattttctga
     4621 actcttcgct gaataaacag gaaattgaga aagttaaaca agatattgtt tcgggaaaaa
     4681 ccaaattact gtatgttgct cccgaatcac ttaccaagat ggagaatatt gattttctcc
     4741 aaaacgtgcc catctcgttt tacgcggtgg atgaagcaca ctgtatatcg gaatggggac
     4801 atgattttcg ccccgaatat cggcgcataa aacccattat caacgaaata ggccctcgtc
     4861 cagtcgttgc gttgacggca acggccacac ccaaggtgca gcatgatatt cagaaaacac
     4921 tgggtatgct ggacgcagcc gtttttaaat catccttcaa ccggtccaat ctatactacg
     4981 aagtaagaaa gaaaaccgat aaggttgata aggagatcat aaaatatatc ctttctcaag
     5041 gcataaaatc aggtattgtt tattgcctga gccgcaaaaa ggtagacgat tttgcacaga
     5101 tactgcaagc caacgatata cgggccctgc cctatcatgc cgggatggac gctgccactc
     5161 gaagcgccaa tcaggatgcg tttctgatgg agcaggcaga cgtaattgtc gctactattg
     5221 cttttggcat ggggatcgac aaacccgatg tacgctatgt gattcattat gacataccta
     5281 aaagtctcga agggtactac caggagacgg gccgttcagg ccgcgacgga ggagaaggca
     5341 aatgtattgc tttctattcc agcaaagatt tgcaaaaact ggaacgtttt atgcagggca
     5401 agcctgtttc cgaacaggaa atcgggaaac aactgctgtt agaaacagcc gcctatgccg
     5461 aaacttccgt ttgccggagg aaaatgctct tgcattattt cggcgaagat tacaacgagc
     5521 ccaattgtgg gaactgcgat aattgtttaa atccaaaaat aaaagtggaa gctaaagaat
     5581 tacttgtcac cgttttagag gccatctccg tgctaaagga aaagcacaag gccgattatt
     5641 tgattaattt tttactcgga aaagaaacat ccgaaataga aacatttgaa cacaatgagt
     5701 tagaagagtt cggatcagga tcggatgaag aggagagcac ttgggaaacg gttatccgtc
     5761 aagcactact tgacaattat ttaaaaaagg acatcgagaa ttacggaatt cttaagatca
     5821 caaagaaagg aaaagaattc ctgaaaaaac ccgtttcttt caaaatcacg aaagaggaag
     5881 atgaagatga tgccgatatc gaagaactcg aatccgaaga aggcgtaatc gcatcgggag
     5941 ggggaaattc cggagctgcc gacccggcat tattttcgat gatgaaagac ttacgaaaga
     6001 aaatatccaa aaaactcaac gtcccgccgt atgttatttt tcagcccgct tccctggaag
     6061 cgatggctac gtcttacccc attacactgg aagagctgca gaatattccg ggtgtagggg
     6121 ccggcaaagc aaaacgttat ggtcaggagt ttatcgaact cataaagaaa catgtagaag
     6181 aaaacgaaat tatccgtccc gaggatttga gggtgaagac cgtagctaac aaatcaaaac
     6241 tcaaggtttc tatcgtacag gctatcgaca gaaaagttgc cctggacgac ctggccgaat
     6301 ccaaaggaat agatttcaac gagatgcttt cggaagttga ggctattgtc tactcgggta
     6361 caaaaataaa catcaactac tttctcgaag aagtaatgga tacggatact gttagagaaa
     6421 tctacgagta tttccgggaa gccgaaaccg ataacctgga taaggctatg gacgaacttt
     6481 ccgattattc cgaaacagaa atccgcttgg tccgaatcaa gtttttctcc gaaatggcga
     6541 attaacacta gacaatgaac catcgaactc gattcttcgt ttatcagaaa cgaagagaag
     6601 agaaaacaaa aagaatgaca tatttacaag acataaatag cccggctgat ctcaaaaagt
     6661 tcaatgttga acaactcaag gaggtctgca aagatttgag ggagttcatt attgaacagc
     6721 tttctcacaa tcccggacat tttgcatcga gtttaggtac tgttgaactc accgttgcgc
     6781 ttcactatct atacaacacg ccatacgaca aactggtttg ggatgttggc catcaggctt
     6841 acggacacaa gatactcacc ggacggcgcg atgttttcca cacaaaccgc aaactgggtg
     6901 gactttccgg atttaccaat ccgcaggaaa gcatatacga tacttttgtt gccggacacg
     6961 cctccacttc cgtgtccgcg gcggtcggga tcgccatggc ccgc
//
LOCUS       DAOSDR010000147         7001 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrimonas sp. isolate AMR_MDS_1826
            NODE_3931_length_7001_cov_4.393608, whole genome shotgun sequence.
ACCESSION   DAOSDR010000147 DAOSDR010000000
VERSION     DAOSDR010000147.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863355
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrimonas sp. (wastewater metagenome)
  ORGANISM  Petrimonas sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Dysgonomonadaceae; Petrimonas.
REFERENCE   1  (bases 1 to 7001)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7001)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:10:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,720
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 35
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7001
                     /organism="Petrimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3931_length_7001_cov_4.393608"
                     /isolate="AMR_MDS_1826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2023866"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..3247)
                     /locus_tag="PLI30_06975"
     CDS             complement(<1..3247)
                     /locus_tag="PLI30_06975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019538130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004866 - endopeptidase inhibitor activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MG2 domain-containing protein"
                     /protein_id="HOI79332.1"
                     /translation="MKKQPLLYIYVAFICTIIFFSFVSCKQKTTQIDPEFARYIAAFT
                     YGNISPDSYIEIELAQELPSVELNTEVQEKFFSFLPFIKGKTYWINSRTIRFVPEYGQ
                     LKPGKEYTAKFYLDKALKVEPKFKTFNFYFRVNEQNFSFDLLPYSPMSINDLKWNSVT
                     GALRLANNESPEKIVGIFELKGANREAKVNVKTVSDGMYQISIDSLLRTNNAESYELT
                     VNGNAIGAKKRENFSIQLPSLPETEFQVIDVRMISETASHIRIAFSSPLSHDQDIQGL
                     VVPSGISNFTYQIDKNVLKIFPEAYPREEITLQIHQGLRSFENRALARDYTYQLRAES
                     EKPQVKMGKSGNILPNSSQLVLPFSAVNLWAVDVKVIKIYQNNILYFLQSNSMNSNSN
                     GEVRRFGRLIKKKQIRLDTDKALDLTRWNNFSIDLAPMINEDPGAIYIVQLSMKSDYS
                     LFNCGGITPQIPQSSSMRNFEDENISEEDEAVWDETNPYYYEPIDWAEYNWEERDDPC
                     KSTYYMNRDRIIETTVMASNMGIIAKGGDENKILIAVTDILSTSPISGADVTVYNYQM
                     QAIGKGKTDGQGFAEIDYKNGKPFVVTATNGKDIGYLEVKEELSLSLSNFDVSGKEIQ
                     KGLKGSVYGERGVWRPGDTIYLSFILEDRAKKLPEGHPVTLEVYTPTRQFYHRQVKTN
                     GENGFYTFQIVTDPLAPTGVWQSYVKVGGTSFYKPLRIETVKPNRLKVRLETDSIIDA
                     SKGVFSGTLTSQWLHGAPASNLKSEVEITLSRTENPFSGYNKYVFNNPLFKFETNSYK
                     LFEGTLNASGVAGVNSRIPVAESAPGMLRGNIVSRVFETGGDMSFYAQTAYYSPYGVY
                     AGVKTPETEASGFLETDKPIVFDVVTLDPYGKKVSRDNVEYKIYKLNWSWWWNSSEED
                     LGSYVNNTAVSPVANGVLSTSGGSGKVKFQVDYPDWGRYLILVKDAGSGHTAGTIFYV
                     DWASTYGRSNKTDPNGLTMLSFSTDKETYAVGETVTVIIPKSSSGRALISIENGSGII
                     WKEWIKTSETEDTEYQFKITEEMNPNFYLFVTLLQPHAQTENDLPIRLYGVR"
     gene            complement(3321..4052)
                     /locus_tag="PLI30_06980"
     CDS             complement(3321..4052)
                     /locus_tag="PLI30_06980"
                     /inference="COORDINATES: protein motif:HMM:NF024245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HOI79333.1"
                     /translation="MHWKDFLYYRKSEKLAVILLLILIVLTLILNLILSQRNSRGVIF
                     TQNDSLVKVFEEFQRNLSLREGIDVLDSAHVVPQNEGRSNDGLRSKKLSEENSPDRDA
                     PFPTAEKLDAGETILLNAADTAEWKKIPGIGSTFASRIVKYRNLLGGFVSVEQLREVY
                     GIDNELFSRISPYIQADENFRKLWINELEFKELLRHPYLNYKQVRAIMNLRKKKGNIA
                     SIHELAMLDEFTSEDIFRIEPYLAF"
     gene            4140..5372
                     /locus_tag="PLI30_06985"
     CDS             4140..5372
                     /locus_tag="PLI30_06985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016274990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside recognition domain-containing
                     protein"
                     /protein_id="HOI79334.1"
                     /translation="MVLNYIWIAFFLIAFVVALARLVFFGDVNVFTEIMNSTYDSAKT
                     GFEISLGLTGILSLWLGVMKIGERGGMIDLFSRGVAPLFSKLFPDIPKGHPASGSMLM
                     NISANMLGLDNAATPFGLKAMQELQEINPDKGKASNPMIMFLVMNASGLTLIPVTVMV
                     YRAQMGAVNPADVFIPIMIATFAATLVGVIAVCLKQRINIFNKAIMGFFLGMLVIIGG
                     TIFAFNSMPQEKANIVSGLAANIILLSVIVLFIVVALRKRVNIFESFIDGAKDGFKTA
                     VTIIPYLIAILVGIGVFRASGAMDFLMDGLRNLVALTGMDTRWVDGMPTAIMKPLSGS
                     GARGMMIDAMKTFGADSFTGRLSSVLQGATDTTFYIVAVYYGAVNIKNSRYTVPYALL
                     ADLAGVLTAVFVAYLFFG"
     gene            5411..5833
                     /gene="ybeY"
                     /locus_tag="PLI30_06990"
     CDS             5411..5833
                     /gene="ybeY"
                     /locus_tag="PLI30_06990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008767506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HOI79335.1"
                     /translation="MAITFQADKINFPKIRKGDIKNWIKRVAASYGKTIGEVNYLFCT
                     DDKILEVNTQYLSHDFYTDIITFDYSEGDKISGDIIISLETVRTNSQKYNTDFTEELH
                     RVIIHGILPMCGIDDKSETASLNMRKAENQALEQINPQ"
     gene            5903..6853
                     /locus_tag="PLI30_06995"
     CDS             5903..6853
                     /locus_tag="PLI30_06995"
                     /inference="COORDINATES: protein motif:HMM:NF040322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI79336.1"
                     /translation="MTTPFSFMFYNTENFYDTSDDPGVMDSEFTPGGRLRWDEKRFND
                     KVTKLTFVIQDIVKPEIPDIIGLAEIENKNVMMSILDDLHRKGMKHYSFVHYDSPDER
                     GSDVAMIYNTQSFIVLESSPVLVHLPGIEDRTRDILHVKGKTTFDEIFHIFIVHFPSR
                     REGTDRSERRRYFVASELHSAVYKVLSENPDENILIMGDFNDTPDDNSVDEVLGAQKS
                     FDNISNTKLYNLIYPRHKKGIGSTYHKGWLLFDQFIASGHVLLSGKFDCKPENADVFN
                     PKYLLHFDKKGRPNTNRTYRNHYTGGFSDHLPIYLKIYVK"
BASE COUNT         1878 a         1591 c         1506 g         2026 t
ORIGIN      
        1 ttctcacacc atacaagcgg atgggtaaat cgttttcggt ttgcgcgtgc ggttgaagta
       61 acgtgacgaa aagatagaaa ttggggttca tctcttcggt aattttgaat tgatattcgg
      121 tgtcttcggt ttccgacgtt tttatccatt ctttccagat gatgcccgaa ccgttttcga
      181 tacttatcaa agcacgtccc gatgagcttt tcggaatgat aaccgtaacc gtttcgccca
      241 cagcgtaggt ttctttatcg gtagagaatg acaacatggt caagccgttc ggatcggtct
      301 tattggaacg tccgtacgtc gatgcccagt ccacataaaa gattgttcct gccgtgtgtc
      361 cgctcccggc atctttcacc aggatcaggt aacgacccca atcgggataa tcaacctgaa
      421 atttaacttt tccgcttccg cccgatgtcg aaagaactcc gttggcaacg ggcgagaccg
      481 ccgtgttgtt cacatacgag cccaaatctt cttcggagct gttccaccac cacgaccagt
      541 taagcttgta gattttatat tctacattgt cgcgcgaaac ctttttccca taaggatcca
      601 aggtaacaac atcaaaaacg atgggtttgt ctgtctccag aaagcccgat gcttctgtct
      661 ccggtgtttt cactccggcg tacacgccat aaggcgagta ataagcggtt tgcgcgtaaa
      721 aactcatgtc gccgccggtt tcgaaaacgc gtgacacaat attgccccgc aacatgcccg
      781 gtgcactttc agcaaccgga atcctcgaat tcacacccgc cacacccgat gcattaagcg
      841 ttccttcaaa caacttgtag ctgttggttt caaacttgaa aagcggattg ttgaaaacat
      901 atttattgta tcctgaaaac ggattttccg tacgcgaaag agtgatttcc acttccgatt
      961 tcaagttaga tgccggcgca ccgtgcagcc actgcgacgt gagcgttccc gaaaaaacac
     1021 ctttacttgc atcgataatg gaatcggttt ccaaacgtac tttcaaccgg ttcggcttaa
     1081 cggtttctat ccgcaagggt ttgtagaacg aagtgccgcc cactttcacg taagactgcc
     1141 aaacaccggt gggtgctaac ggatcggtaa caatttgaaa cgtgtaaaat ccgttttcac
     1201 cgttggtttt cacttggcgg tgataaaact gacgcgtggg tgtgtaaact tcgagcgtaa
     1261 cgggatgtcc ttcgggcagt ttctttgccc tgtcctccag gataaacgac aagtagatgg
     1321 tgtcgccggg acgccaaaca cctcgctcgc catatacaga accttttaaa cctttctgga
     1381 tctcttttcc gcttacatca aaattactca gcgaaagcga aagttcttcc ttcacttcca
     1441 gatagcctat atcttttccg ttggtggcag taaccacaaa cggtttcccg tttttataat
     1501 caatttcggc aaaaccctgt ccatcggttt tcccttttcc tattgcctgc atctgatagt
     1561 tataaaccgt tacatcagca ccggaaatag gtgatgtgga aagaatatcg gtaacagcga
     1621 tcaggatttt attctcgtct ccgcctttgg caataatccc catgttggat gccataaccg
     1681 tggtttcgat aatgcggtcg cggttcatat aataggtgga tttgcacggg tcgtcacgct
     1741 cttcccaatt gtattctgcc cagtcgatgg gttcgtaata atacgggttc gtttcgtccc
     1801 aaaccgcttc atcttcttcg ctgatgtttt cgtcttcaaa attacgcatc gatgagcttt
     1861 ggggaatctg tggcgttata cctccacaat tgaaaagcga ataatcggat ttcatagaga
     1921 gttgcactat atagatagct cccgggtctt cgttaatcat cggtgccaag tcgatagaaa
     1981 aattgttcca gcgggttaaa tccagggctt tatcagtatc taaacgaatt tgtttctttt
     2041 tgattaaccg tccgaaacga cgtacttcgc cattcgaatt gctgttcatt gaattcgatt
     2101 gcaagaagta aagaatatta ttctgataaa tttttatcac tttcacatcc acagcccaca
     2161 aattcacagc actaaacgga aggactaatt ggctggaatt gggcaggata tttccgcttt
     2221 ttcccatttt cacctgtggt ttttcgcttt cggcacgaag ttgataggta taatctcttg
     2281 cgagtgcccg attttcgaaa ctccgtaacc cttgatgaat ttgcaatgta atttcttccc
     2341 gcgggtaagc ttcgggaaat attttcagta cgtttttatc gatttgatac gtaaaattac
     2401 tgattcctga aggaacgacc aatccctgaa tatcctggtc atgtgacaaa gggctactga
     2461 aagcgatacg aatgtgagaa gcggtttcac ttatcatccg cacatcaatc acctgaaatt
     2521 cggtttcggg caatgaaggg agctgaatgc taaagttctc cctttttttg gctccgatag
     2581 cgtttccgtt caccgtaagt tcatagcttt ccgcattatt tgtccgaagc aaactatcta
     2641 tcgaaatctg atacattccg tctgaaaccg ttttcacatt aacttttgct tcacgattag
     2701 cccctttcag ttcaaagata ccaacgattt tctcgggtga ttcgttattg gccaatcgta
     2761 atgctcccgt tacggagttc catttcaaat cattgatact cataggagaa tagggtaaca
     2821 gatcgaacga gaagttttgc tcgtttaccc tgaaatagaa attgaaagtc ttaaacttcg
     2881 gctccacttt caacgcttta tccaaataaa atttcgccgt atattctttt ccgggtttta
     2941 attgtccgta ttcgggaacg aatcgtatgg ttctcgaatt gatccaatat gttttccctt
     3001 tgatgaaagg caagaacgag aaaaacttct cttgcacctc ggtatttaat tccacggaag
     3061 gtagttcctg tgcaagctcg atttcgatgt acgaatcggg cgaaatattg ccgtaagtga
     3121 atgccgctat atatcgggca aattcggggt cgatctgcgt tgttttctgt ttgcaggaaa
     3181 caaacgagaa gaaaattatc gtgcaaataa atgcaacgta aatataaaga agaggttgtt
     3241 ttttcatatc gatttttttt ctgaagattt ttttagccga gttattttat gagcaggctg
     3301 tcgtgaatgt atggcggaag tcagaatgcc aggtagggtt cgatccgaaa aatgtcttcg
     3361 gaagtaaatt catccaacat agccagttca tggatggaag caatatttcc tttctttttc
     3421 cgcaggttca taatggctcg tacctgtttg taattgaggt aaggatgcct taatagttcc
     3481 ttaaattcca actcgttaat ccaaagcttt ctgaagtttt cgtcagcttg gatatacggc
     3541 gagatcctgg aaaacagttc gttgtcaatc ccataaacct ccctgagttg ctctaccgaa
     3601 acaaatcccc ccagcagatt ccgatacttc acaatccgcg aagcgaaggt actgcctatt
     3661 cccgggatct ttttccattc agccgtatca gcagcattca aaaggatcgt ttctcctgcg
     3721 tctaattttt ctgctgtagg aaagggagca tcgcgatccg gactgttctc ttcggaaagt
     3781 tttttggatc gcaacccgtc gttactcctt ccttcatttt gcggcacaac atgcgctgag
     3841 tccagaacgt ctataccttc acgcaaacta aggttccgct gaaattcttc aaatactttc
     3901 accagcgaat cgttttgcgt gaaaatgacg ccccggctgt tgcgttgcga gagtatcagg
     3961 tttaaaatca gcgtcagcac aatcaggata agcagcagaa ttaccgctaa tttctcgctt
     4021 ttccgataat atagaaaatc cttccaatgc atagacggca gcacttctaa ggggtaaaga
     4081 taaaaattat ttccccgaaa gtattacctt tgacagaata attgtgaaat gcttttgcta
     4141 tggtcctgaa ttacatatgg attgcctttt ttctcatcgc ttttgtagtt gctctggcca
     4201 gacttgtttt cttcggcgat gtaaatgtat ttacagaaat aatgaattcc acgtacgact
     4261 ctgccaaaac tggattcgag atttcactgg gtctgacagg cattctttcg ctttggttgg
     4321 gcgtgatgaa gatcggcgaa cgtggtggaa tgatcgatct tttttcgcgt ggtgtggcac
     4381 cgttattctc caaactcttc ccggatattc ctaaaggaca tccggcgtcg ggctctatgc
     4441 ttatgaacat ctctgccaat atgctcgggc tcgataatgc ggccacccca ttcgggctga
     4501 aagcaatgca ggagcttcag gagataaatc ctgacaaagg caaggcatcc aatccaatga
     4561 tcatgtttct ggtgatgaac gcctcgggac tgaccctgat tcctgtcact gtgatggttt
     4621 atcgtgcaca aatgggtgca gttaatccgg cagacgtgtt tatcccaata atgatagcaa
     4681 catttgctgc caccctcgtg ggggttattg ccgtttgtct caaacaacgg ataaacatct
     4741 ttaataaggc cataatggga tttttcctcg gtatgctggt aataattgga ggaaccattt
     4801 ttgcttttaa ctctatgccg caggagaaag caaacatcgt ttcgggactt gcggctaaca
     4861 ttattctgct ctctgttata gtccttttta tcgtggtggc gttgcggaag agggtcaaca
     4921 tctttgaatc atttatcgat ggtgccaagg acgggtttaa aacagccgtt accatcatcc
     4981 cttacctcat tgctatcctg gttggaattg gtgtttttcg agcatctggc gccatggact
     5041 tcctgatgga tggcctgcgg aatttagtag cacttaccgg aatggatacc cgttgggtag
     5101 acggaatgcc cacggcgatt atgaagccgc tcagcggaag tggtgcccgc gggatgatga
     5161 ttgatgcgat gaagactttc ggagccgatt catttaccgg aaggctctca tccgtcctgc
     5221 agggagcgac agacactacg ttttacattg ttgctgttta ttacggagcc gtgaacatca
     5281 aaaacagccg ctataccgtt ccctatgcgc ttttggcaga tttggcaggt gttttgactg
     5341 cggtttttgt tgcctacctt ttcttcggat aaaaacaagc tcttctctgc gtaaataaaa
     5401 gttaaaaaat atggcaatta catttcaggc tgacaaaatc aattttccga agatcaggaa
     5461 aggcgatatt aaaaattgga ttaaacgtgt tgccgccagc tacggcaaaa caatcggcga
     5521 ggtcaattat ctgttttgta cggatgataa aattctcgag gtaaacaccc aatatctttc
     5581 ccacgatttt tacaccgaca ttatcacgtt cgactattcg gaaggagata aaatatcggg
     5641 cgatatcatt atcagcttag agacggtccg cactaattca caaaagtata acaccgattt
     5701 tacggaagaa ctacatcgtg ttattattca cggaatcctc cccatgtgcg ggattgatga
     5761 taaaagcgaa acggcttccc tcaatatgag gaaggcggaa aaccaggctt tggaacaaat
     5821 aaacccgcaa taattacagt tctctttatc agtaatttac ttttggaaac atacaaatgt
     5881 taatcaccta aaataaaccg taatgacaac cccgttttct tttatgtttt ataataccga
     5941 gaatttttac gatacttcag atgaccctgg agtaatggac agcgaattca ctccaggggg
     6001 aagattaaga tgggatgaga aaagatttaa cgacaaggta accaagttaa catttgtaat
     6061 tcaagacatt gtaaaaccgg aaattcccga tattatcgga ctggccgaaa tcgagaacaa
     6121 aaacgtgatg atgagtattc tggacgattt acacagaaaa ggaatgaagc actacagctt
     6181 tgttcattac gacagtcccg acgaacgcgg cagcgacgta gccatgatct acaatacaca
     6241 atcattcatc gtactcgaat caagtccggt tcttgttcac ctaccgggta ttgaggatcg
     6301 tacccgcgat attctccacg tgaaaggcaa aactacattt gacgagatct tccacatctt
     6361 tattgtgcat ttcccttcgc gaagggaggg gacagacaga tcggagcgca ggcgctattt
     6421 cgtagcaagc gagctgcaca gtgccgttta caaggtatta agtgaaaacc ccgatgagaa
     6481 catacttatt atgggagatt tcaacgacac tccggacgac aacagcgttg acgaggtgct
     6541 cggggcgcaa aaatcattcg acaacataag caacaccaaa ctctataacc ttatataccc
     6601 taggcacaaa aaaggtatcg gatcaaccta tcacaaaggc tggctgctgt ttgaccagtt
     6661 tatcgcttcg ggacatgtgc ttctgtccgg taagtttgac tgcaagccag agaacgccga
     6721 cgttttcaac cccaaatacc tgctccattt tgataaaaaa ggccgcccca acaccaaccg
     6781 gacctaccgg aatcattata ccggtggctt tagcgatcac ctgcctatct acctgaagat
     6841 ctatgtgaag taagtttaat ttcactcaat taacatctta taagcgcatt agcagcttct
     6901 tttttcgtat atttgcagcc gcaaaaaaga agcaaaaggc ttaccatgcg gatgtggcgt
     6961 aattggtagc cgcgccagac ttaggatctg gtgccgagag g
//
LOCUS       DAOSDR010000148         6978 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrimonas sp. isolate AMR_MDS_1826
            NODE_3949_length_6978_cov_3.919544, whole genome shotgun sequence.
ACCESSION   DAOSDR010000148 DAOSDR010000000
VERSION     DAOSDR010000148.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863355
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrimonas sp. (wastewater metagenome)
  ORGANISM  Petrimonas sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Dysgonomonadaceae; Petrimonas.
REFERENCE   1  (bases 1 to 6978)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6978)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:10:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,720
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 35
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6978
                     /organism="Petrimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3949_length_6978_cov_3.919544"
                     /isolate="AMR_MDS_1826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2023866"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..982
                     /locus_tag="PLI30_07000"
     CDS             <1..982
                     /locus_tag="PLI30_07000"
                     /EC_number="1.1.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019540388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016901 - oxidoreductase activity, acting
                     on the CH-OH group of donors, quinone or similar compound
                     as acceptor [Evidence IEA];
                     GO_function: GO:0070968 - pyrroloquinoline quinone binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HOI79337.1"
                     /translation="LPDGRLAITEKGGTMRIATTEGTVSPPISGFPAVDDRGQGGLLD
                     VAPAPDFEASRMLYFTLAEKTTQGSLTAVGKGKLSADETTIENFQIIFRAVPYFDNSM
                     HFGSRIVFDKRGNIFVSTGERSDLRTRPNAQLLNTGHGKVIHITTEGKPVAGNPFINT
                     EGALPEIYSYGHRNPQGLDIHPLTQELWLSEMGPRGGDEINLVKPGKNYGWPTITYGI
                     EYSGAAISGGATQKEGLEQPVYYWDPVLSPSGMAFYSFSAIPEWQNNLFIGGLNSKYI
                     ARIVLKDNKVIGEERLLAGENQRFRDVGDGKDGAIYAVTDEGRLYRIAKK"
     gene            complement(1018..1437)
                     /locus_tag="PLI30_07005"
     CDS             complement(1018..1437)
                     /locus_tag="PLI30_07005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HOI79338.1"
                     /translation="MAKLNPYLNFDGTCEEAFRFYKVVFGGEFLGDVHKMKDIPGMEI
                     PEEAKNRIMHVALPVGNDLLMGSDVFPGQPFIQGNNNYISLFPESREEADRLFGALSE
                     GGEVEMPMADQFWGDYFGSLKDKFGVYWMINYNSANQ"
     gene            complement(1493..3376)
                     /locus_tag="PLI30_07010"
     CDS             complement(1493..3376)
                     /locus_tag="PLI30_07010"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019539627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HOI79339.1"
                     /translation="MKFRIIFFSLLVTMAISAQRTQKPELHGKHWMAITGKSLGASAG
                     AMIFQQGGNAVDAACAMLGATCTLWDVLSWGGETQALIYNPKTEKVIAINALGVAPTG
                     ATAGFFKDQGCDFPPEYGPLAAVTPGTAGGLCYMLANYGTMSLKDVLAPSIELAKGFP
                     VDVPFETSVSRDKERLSQWKYSKKVFITRPSEEKETPVSGEIFVQENLAETLQKLVDA
                     EQQALKEGISRKEAIMAAYDRFYKGDIAAEIVRSVREQGGLFTMEDLARWKPVEEEPL
                     HTNYKGIDVFKLQSWTQGAAMLQSLNILENFDLRQMGYNSPKYIHTVYQAMNMAFADR
                     DFYYGDPYVHPDIPIKGLLSKEYSRKRASEMWHDKNNPSVGPGDPYPFEGKKNPFIKI
                     LKERGFDRDPVEDRDFVPKHDVVSLSEMDSLYQDRLHRGTTSIVAADAEGWVVSVTPS
                     GGWIPACIAGSTGIGLSQRMQSFVLDESLNPFNVVAPGKRPRVTLTPSLALKEGKPFL
                     AFAVQGGDTQEQNLLQFFLNIVEFGMNTQQAAEAANINSNQLWLSLGGTRTEDRSPRA
                     GQILLNTQTPGPTRQELTAMGYMLQFSDRTSGPVNAIYFDRKNGTMRGGSSNHGEDYG
                     IAW"
     gene            complement(3366..3800)
                     /locus_tag="PLI30_07015"
     CDS             complement(3366..3800)
                     /locus_tag="PLI30_07015"
                     /inference="COORDINATES: protein motif:HMM:NF019928.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HOI79340.1"
                     /translation="MYKKQISINTSVDKVWNALTQPEEMRNWYFSISNFEATEGAVFD
                     FIVSFTDEAGEHSFRHLFKILEVIPNEKLRHTWEHPGHSEGTSTLTWELIPGEETTTV
                     ILTHEGNESFLDEGSKYFTAESYTAGWNDILQGLKDYLENEV"
     gene            complement(3979..4821)
                     /locus_tag="PLI30_07020"
     CDS             complement(3979..4821)
                     /locus_tag="PLI30_07020"
                     /inference="COORDINATES: protein motif:HMM:NF018663.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HOI79341.1"
                     /translation="MNNTIYPCIWCNNNAREMANFYCSTFPETTVMDENPVVVMLEMF
                     GQKLMLLNGGDKFNPNPSISLMFLTMLEKRVEEIWNKLIQEGRSMMPLDAYPFSPKYG
                     WVQDKYGVSWQLYTARDENHILQKIVPTLMFTGSQNGRAMEAAHLYTSLFPNSEIHGV
                     MYYAEESGEPESSVQHGEFFINDYLLMMMDSSQEHNFSFSEGVSLVVECDYQEEIDNY
                     WNVLTSNGGEESMCGWLKDQFGVSWQIVPAELGGWMKKSTKVMAEVLKMKKLDIDTLK
                     NAIK"
     gene            complement(4823..5185)
                     /locus_tag="PLI30_07025"
     CDS             complement(4823..5185)
                     /locus_tag="PLI30_07025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HOI79342.1"
                     /translation="MSSPENIDQYMAGFDAEVRKKLRTIRELVHDTLPEAVESISYQM
                     PAFKYNGKILVYFAAFKNHIGFYATPESNLAFKEELSEYKTTKGTIQLPYDKPLPIEL
                     LRKIILFKAASNEVKKKK"
     gene            complement(5217..5783)
                     /locus_tag="PLI30_07030"
     CDS             complement(5217..5783)
                     /locus_tag="PLI30_07030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008022672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdeI/OmpD-associated family protein"
                     /protein_id="HOI79343.1"
                     /translation="MEITKAVYFDSREDWRQWLSENFRKEKEIWLIYPNKSTRKPRIL
                     YNDAVEEALCFGWIDSTMKKYDETHTAQRFSPRNPKSTYSQPNRERLRWLAENNLLHP
                     EIYEKVTNILSEEFVYPSDIITALQADKETWQNFQRFSEAYKRIRVAYVHDSRSRPDF
                     FAKRLANLLKMTKQNRKIIGHGGVDKYY"
     gene            complement(5792..6328)
                     /locus_tag="PLI30_07035"
     CDS             complement(5792..6328)
                     /locus_tag="PLI30_07035"
                     /inference="COORDINATES: protein motif:HMM:NF019614.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1697 domain-containing protein"
                     /protein_id="HOI79344.1"
                     /translation="MTKYIVLLRGVTPTGKNRVPMAQLRQLLAESGFQNVQTWIQSGN
                     VILYSELSVEEVVKRVNKIIKESIGADLKIIVKTPAEIETVIAENPFGKRYDISRVFF
                     TLFNDTPDINLVTALQAQDFGPDEFIFTPHAIYLFIPGSAAKTKLNNNFLERKLKIDT
                     TTRNFNTLSKLIALSSNP"
     gene            complement(6332..>6978)
                     /locus_tag="PLI30_07040"
     CDS             complement(6332..>6978)
                     /locus_tag="PLI30_07040"
                     /inference="COORDINATES: protein motif:HMM:TIGR01777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TIGR01777 family oxidoreductase"
                     /protein_id="HOI79345.1"
                     /translation="MDKRPEAFISASAIGYYGLKITNNLYSEEDPSTENDFLSRTCRK
                     WENAALRFQEELNIRTVVLRTGFVISKNSDAFRKMVLPTRFGLGAPIGSGRQYLTWVH
                     IDDLCRMYLKAIEDVDMKGVYNAVSPEFISNADFMHTLANVMKRPFFMPHVPSFLMRL
                     IMGEAAGMILGGSRISSRKIQDAGYEFQYDTSEKAMKASLKAIKERENKKRKRN"
BASE COUNT         1800 a         1717 c         1476 g         1985 t
ORIGIN      
        1 ccttcctgac gggaggttag ccatcactga aaaaggaggc acaatgcgca tagccaccac
       61 tgagggtaca gtcagccccc ccatctccgg atttcctgcc gttgacgaca ggggccaggg
      121 agggttgctc gacgtagctc cggctcccga ttttgaggcc agcaggatgc tttacttcac
      181 ccttgccgaa aagacaacgc aaggatcgct tacagcagtg ggtaagggca agctctctgc
      241 tgacgaaaca accatcgaga attttcagat cattttcagg gccgttccat acttcgacaa
      301 tagcatgcac ttcggaagcc gcatcgtttt tgacaagcgt ggaaatattt tcgtttccac
      361 cggtgaacga tcggatttga gaacaaggcc caatgcacaa ttactcaata ccggacacgg
      421 gaaagtaatt catatcacca ccgaagggaa accggttgcc ggtaatccgt ttatcaatac
      481 agaaggtgca ttgcccgaga tttactccta tgggcatcga aatccacaag gactcgacat
      541 ccatccgctt acccaggaac tctggttatc ggaaatgggg ccgcgaggcg gtgatgaaat
      601 caacctggtc aaacccggca aaaattacgg atggccaacc atcacgtatg gaattgaata
      661 cagcggtgct gccatcagtg gtggagctac gcaaaaagaa ggactggaac agccggtata
      721 ttattgggat cctgtcctct ctcccagcgg aatggcattc tattctttca gtgcaattcc
      781 agaatggcag aacaacctgt ttataggtgg gttgaacagc aaatacatag cccgaatagt
      841 cctgaaagac aataaagtta tcggtgaaga aagactgctc gccggcgaaa atcaacgatt
      901 tcgagatgtc ggcgatggaa aggatggagc aatttatgcc gttaccgatg aaggccggct
      961 gtaccggatt gcgaagaaat gaaacaaccc ggaacagcgg gcaataaacc agacagatta
     1021 ctgatttgca gaattataat taatcatcca gtagactcca aatttatctt tcaagcttcc
     1081 gaaataatca ccccaaaact ggtcagccat aggcatttcc acttcaccgc cttccgacaa
     1141 agctccgaat aaacggtcag cctcttccct gctttcggga aagaggctga tatagttatt
     1201 attcccctga ataaacggtt gccccggaaa cacatcagaa cccattaaaa gatcatttcc
     1261 gacaggaagt gctacgtgca taatcctgtt tttggcttct tcaggtattt ccatgccggg
     1321 gatgtctttc attttgtgaa catctcccag aaattcaccg ccaaaaacaa ctttataaaa
     1381 acgaaatgct tcttcgcaag taccgtcaaa attgaggtaa ggatttaatt tagccataat
     1441 ttttttgatt tttaaattgt tacaaacttg tgatgtaatg gatcattact actcaccacg
     1501 caattccgta atcctcgccg tggttactgc ttccaccgcg catagtcccg ttcttccggt
     1561 cgaaataaat ggcattcact ggtccgcttg tccgatcaga aaactgcagc atatatccca
     1621 ttgccgttaa ctcctgacgg gtcggaccgg gcgtttgtgt gttgagtaaa atctgccctg
     1681 ccctgggact acgatcttca gttcttgtgc cgcccagcga caaccacaac tggttggagt
     1741 tgatatttgc tgcttcagcc gcctgctgcg tattcattcc aaactccact atattcagga
     1801 agaactgcaa caggttttgt tcttgtgtat ctccaccctg tacggcaaaa gccagaaacg
     1861 gctttccctc cttcaacgcc aggctgggtg taagcgtaac cctcggacgt tttccaggtg
     1921 ccaccacgtt gaatgggtta agtgattcgt cgagaacgaa actctgcatc cgctggctca
     1981 accctattcc tgtgcttccg gcgatgcagg caggaatcca tccaccggaa ggtgtgaccg
     2041 agaccaccca tccttcagcg tcggcagcta ctattgaggt tgttccccga tgtaacctgt
     2101 cctgataaag ggagtccatc tctgagagcg aaacgacatc gtgcttggga acaaaatccc
     2161 tgtcttctac cgggtcccgg tcaaatccgc gttccttcag aatctttata aatggatttt
     2221 tctttccttc gaacggatac gggtccccgg gcccaaccga cgggttattt ttatcatgcc
     2281 acatttcgga cgctcgcttt ctggaatatt ccttgctcaa taatcctttg atgggaatat
     2341 cgggatgaac atagggatca ccgtaataga aatcacgatc cgcaaaagcc atgttcatgg
     2401 cctgataaac cgtgtgaata tacttcgggc tgttataccc catttgcctt aaatcgaagt
     2461 tttccaggat gttcaggctt tgcaacattg ccgctccttg cgtccacgac tgcagcttga
     2521 acacatctat tcccttgtaa ttcgtgtgca ggggttcttc ttcaacaggc ttccagcggg
     2581 ccaggtcttc catcgtaaac agccctccct gttctcgtac gctccgaaca atctccgctg
     2641 caatatctcc tttgtagaac ctgtcgtaag cggccattat ggcctccttc cggcttatac
     2701 cttctttcag ggcctgttgc tctgcgtcaa caagtttttg caaggtctcc gccaaattct
     2761 cctgaacaaa aatctccccc gacaccggcg tttctttttc ttcactcggt ctggtgataa
     2821 aaaccttttt gctgtatttc cactgtgaca atcgttcttt gtccctcgag acgctggttt
     2881 cgaaagggac atcaaccggg aatcctttcg ccagctcaat agaaggagcc aaaacatcct
     2941 tcaggctcat cgtaccgtag ttggcaagca tataacacaa accgcctgcc gtaccgggtg
     3001 tgacggcagc caacgggccg tattcgggag ggaaatcaca cccctgatct ttgaagaaac
     3061 cggcggtagc tcccgtaggt gcaacgccaa gggcatttat agcgatcacc ttttccgttt
     3121 ttggattgta gatcaaggct tgcgtctcgc caccccagct tagcacatcc cacaacgtgc
     3181 aggtagctcc caacatagca caggcggcat ccaccgcatt accaccctgc tgaaagatca
     3241 tggctcctgc cgaggctccc aggctttttc cggtgatggc catccagtgt ttcccgtgta
     3301 attccggctt ctgggtacgc tgcgcagaaa tggccatcgt taccaagaga gaaaaaaaga
     3361 ttattctaaa cttcattttc gagatagtcc ttcagtcctt gcagaatatc gttccatccg
     3421 gcagtgtagc tttccgcagt gaaatatttg ctgccctcat cgagaaaact ttcgttcccc
     3481 tcgtgtgtaa ggatcacggt agtggtctcc tctccgggaa taagctccca ggtaagcgtg
     3541 gaagttccct cactatgtcc gggatgctcc caggtatgtc tcagtttttc gttagggatc
     3601 acttcgagta tcttgaataa atgacggaaa ctatgctctc cggcttcatc ggtaaaagaa
     3661 acaatgaagt cgaatacggc accttccgtc gcttcaaaat tcgaaatact gaaataccag
     3721 tttcgcattt cttccggttg cgtcagtgcg ttccacactt tatcaacgct tgtgtttatt
     3781 gaaatttgct ttttatacat acgattgttc agataaacgg ttaaacacct caaaaaaata
     3841 tcatcaaatt tctatattga tacgcacttc ccagcattat cccgatgaca gcacccgacg
     3901 gttcttccaa caaaacaaaa aaagtacttt gtttatcgat caccgcataa tcggcaaaag
     3961 acccatgttt agaagttatt attttatggc atttttcaat gtatcaatat ccaatttttt
     4021 cattttcaat acttctgcca tcacctttgt tgattttttc atccatccac ccagctcggc
     4081 aggaacaatt tgccaactga ctccgaattg atcttttagc catccgcaca tactttcctc
     4141 tccgccattc gatgtaagca cgttccaata gttatcgatt tcctcctggt aatcgcactc
     4201 aaccacaagc gaaacacctt cggaaaaact gaaattgtgt tcctgtgagc tatccatcat
     4261 catcaacaga taatcgttga tgaaaaattc tccgtgctgc acgctggatt caggctcacc
     4321 gctttcttca gcatagtaca ttacaccatg gatttccgaa ttgggaaaaa gtgatgtgta
     4381 caaatgcgcg gcctccattg cccgtccgtt ttgagaaccg gtaaacatca gcgtcggcac
     4441 tattttctgg aggatatggt tttcatcgcg tgccgtgtat aactgccacg aaacgccata
     4501 tttatcttgt acccagccat atttcggact aaagggatag gcgtcaagcg gcatcatcga
     4561 cctcccttcc tgtatgagct tattccaaat ctcttctact cgcttttcca acatcgtaag
     4621 aaacatcagc gaaatagacg gattggggtt gaacttgtcg ccaccgttaa gcagcatcag
     4681 tttctggcca aacatttcga gcatgaccac cacaggattt tcatccatta cggtggtttc
     4741 aggaaaagtg ctgcaataaa aattggccat ttcgcgggcg ttgttgttgc accaaatgca
     4801 aggataaatg gtattgttca tattactttt tcttttttac ttcgttggaa gccgctttga
     4861 acaaaatgat tttgcgaagt aattctatcg gaagcggctt gtcgtaaggt aattgaatag
     4921 ttcctttggt ggttttgtat tccgacaatt cttctttgaa tgccagattg gattcaggcg
     4981 tggcgtaaaa tccgatatgg tttttaaatg ctgcaaaata caccagaatt tttccgttat
     5041 atttaaaggc aggcatttgg taactgatag attccacagc ttcgggaaga gtatcgtgga
     5101 ccagttctcg aattgttctc agttttttcc gtacttcagc atcaaagccg gccatatatt
     5161 gatcgatgtt ttcggggcta ctcattgact ttactctttt taaagactgg attgtctcaa
     5221 taatacttat ccactccgcc gtgtccaatt atttttctgt tttgctttgt catctttaac
     5281 aggttggcca gacgttttgc aaaaaaatcg ggacggctgc gtgaatcgtg gacatatgct
     5341 acccggattc gtttataagc ttcagagaat ctttgaaagt tttgccacgt ttccttgtcg
     5401 gcttgcaatg ctgtgataat atctgatggg taaacaaatt cttccgataa aatgtttgtt
     5461 actttttcgt aaatttcagg gtgtaacaag ttattctctg ccaaccaccg aagtcgttcc
     5521 ctgttgggtt gagagtaggt acttttagga ttccggggcg agaatcgctg agcagtgtga
     5581 gtttcatcgt attttttcat cgtactgtcg atccaaccaa aacataaagc ctcctccacg
     5641 gcatcgttgt ataaaatacg cggtttcctt gtcgatttat tggggtagat aagccatatc
     5701 tccttttctt tacgaaaatt ctccgacaac cattgacgcc agtcttcgcg cgaatcgaaa
     5761 tatactgctt ttgttatttc cattatgagg cctaggggtt agagctaaga gctatgagtt
     5821 tcgacaacgt attaaaatta cgggttgtgg tgtcaatttt taattttctt tcgagaaagt
     5881 tgttattgag cttggtttta gcagcactgc cgggaatgaa aagataaatg gcgtgtgggg
     5941 tgaaaatgaa ttcgtctggg ccaaaatcct gtgcttgcag ggctgtaaca agattaatat
     6001 cgggagtatc gttgaaaagc gtaaagaaaa cccgggaaat atcgtatctt ttaccgaaag
     6061 gattttcagc aattaccgtt tcaatctcag caggcgtttt cacgataatt ttcaaatcgg
     6121 caccgatgct ctctttgatg attttattca ctctcttcac tacttcttcg actgaaagtt
     6181 ccgaatagag gatcacgttt ccgctttgaa tccacgtttg tacattctgg aaaccactct
     6241 cagcaagcaa ctgtcgaagt tgcgccatcg gtacccggtt tttcccggtg ggtgttacgc
     6301 cccgaagaag cacgatatat tttgtcaccg gttaatttct ttttcttttt ttgttctccc
     6361 tctcctttat ggctttcaac gaagctttca tcgctttctc cgaagtatca tactgaaatt
     6421 cataaccggc atcttgaatt ttccgggatg aaatgcggct tccccctaga atcataccgg
     6481 cagcctctcc cataatcaga cgcattaaaa acgagggtac gtgcggcata aaaaaaggcc
     6541 gtttcatcac atttgccaac gtatgcatga aatcggcatt actgataaat tccggggaaa
     6601 cagcattgta cacgcctttc atatctacat cttcaatagc tttcaaatac atacggcaaa
     6661 ggtcatcgat atgtacccac gtcaaatatt gcctgccgct cccaataggt gcacccagcc
     6721 caaaacgggt gggcaaaacc attttcctga aggcatcgct gttttttgaa atcacgaatc
     6781 ctgtacgcaa cacgactgtc cggatattga gctcttcctg aaaacggaga gcagcattct
     6841 cccatttacg gcaggtccgg ctcagaaaat cgttttcggt agaaggatcc tcctccgagt
     6901 acaggttgtt ggttattttc aatccgtaat acccgattgc cgaagccgag ataaaagctt
     6961 cgggcctttt atccatag
//
LOCUS       DAOSDR010000149         6965 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrimonas sp. isolate AMR_MDS_1826
            NODE_3957_length_6965_cov_4.148191, whole genome shotgun sequence.
ACCESSION   DAOSDR010000149 DAOSDR010000000
VERSION     DAOSDR010000149.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863355
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrimonas sp. (wastewater metagenome)
  ORGANISM  Petrimonas sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Dysgonomonadaceae; Petrimonas.
REFERENCE   1  (bases 1 to 6965)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6965)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:10:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,720
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 35
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6965
                     /organism="Petrimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3957_length_6965_cov_4.148191"
                     /isolate="AMR_MDS_1826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2023866"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>365)
                     /locus_tag="PLI30_07045"
     CDS             complement(<1..>365)
                     /locus_tag="PLI30_07045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019537931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOI79346.1"
                     /translation="NGFMGMPEVEVVAGCDVYGIKRERFLKRVNDHNVTQQKSVAVEV
                     YENYKDLIAREDIDAVVIATPDHWHAFIAIAACNAKKNVYLEKPLTFTIKEGQELVKA
                     VRNNGVILAVGSQQRSDPN"
     assembly_gap    366..375
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(659..1321)
                     /locus_tag="PLI30_07050"
     CDS             complement(659..1321)
                     /locus_tag="PLI30_07050"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HOI79347.1"
                     /translation="MYNPILADCPLFSGIDDKTLEDFISDTVCEIRTFQKGEYVVRQG
                     DTITFLYLLTEGIVRTEMITAEGNLVEIEFIEAVRPLAPAFIFSDQHRFPVDAITTEK
                     AVVYMIPKSVFLKKMMLNETLLRNFLRLNSNMTVFLSGKVKMLSIKSLRAKLSLYILE
                     NTSPDQDSFILKRTQTQLAEYFGVQRPSLARTIGEMINDGVISLYKKELKVLNRKKLE
                     HY"
     gene            1572..3233
                     /gene="hcp"
                     /locus_tag="PLI30_07055"
                     /gene_synonym="priS"
     CDS             1572..3233
                     /gene="hcp"
                     /locus_tag="PLI30_07055"
                     /gene_synonym="priS"
                     /EC_number="1.7.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016661 - oxidoreductase activity, acting
                     on other nitrogenous compounds as donors [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxylamine reductase"
                     /protein_id="HOI79348.1"
                     /translation="MFCFQCQEAAKNEGCTVKGVCGKTADVANLQDLLLFLCKGISHY
                     TVPLRKYGIEIPQINKFITDSLFMTITNANFDKNRFTTRLLMAFEMRNAARERLTNTG
                     GNIDGITFDGALWVGETEAEISKKAFEVGVLTTKNEDVRSLRELTIYGLKGMAAYAEH
                     AFNLGYTENEIFAFMQRALVATTDDTLSVDELTALVLETGKYGVQVMALLDRANTSSY
                     GNPEITKVNLGVRNNPGILISGHDLKDMEDLLKQTEGTGVDVYTHSEMLPANYYPAFK
                     KYSHFVGNYGSSWWHQTEDFENFNGIILFTTNCIVPPRKSSTYIDRVYTTGASGFEGF
                     KHIGDRKEGQPKDFSAIIEHAKKCAAPKEIETGEIVGGFAHNQVIALADKVVEAVKSG
                     AIRKFFVMGGCDGRMNGRNYYTEFAQKLPGDTVILTAGCAKYRYNKLPLGDINGIPRV
                     LDAGQCNDSYSLVKIALALKEALGLNDLNDLPIAYNIAWYEQKAVIVLLALLYLGVKN
                     IHLGPTLPAFLSPNVVNVLVNNFGITGITSVDEDMKLLLETVECK"
     gene            3383..3673
                     /locus_tag="PLI30_07060"
     CDS             3383..3673
                     /locus_tag="PLI30_07060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007662970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HOI79349.1"
                     /translation="MENSIEYSSGGVVSKQVIKKQSGNVTLFSFDKDQGLTEHTSPYD
                     AMVYLLDGEAEIRIDGNPHHLKKGECIIMPAGFSHALYAIEPFKMVLTMIKQ"
     gene            3678..4868
                     /locus_tag="PLI30_07065"
     CDS             3678..4868
                     /locus_tag="PLI30_07065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018028618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF438 domain-containing protein"
                     /protein_id="HOI79350.1"
                     /translation="MSEVINNSSQRKELLRHFLLRLHEGENPEVLRQRLIEVLRNIPY
                     NEVVEVEQELINSNALTEEEILDFCDLHTAVLDGSIDLGGAKPVPAGHPVDTFKKENQ
                     SLKREVIKIAGLFDNVTDLSEKQLPGFILQLRTIFNNLSDVDKHYKRKEYLLFPFLEK
                     HLITGPPKVMWGKHDEIRDLLKSSFEALESPVSTVEEMKSLVQLVLVPAVEMLDGMIM
                     KEEEILFPMAMDTLTEEEWYKVYQETPNFGYCLFDPEDEWQPSGTKVEKQEYVTADAI
                     RLSSGAFNLAELEALFLHLPIDITFVDKIDKVRFFSHSPNRVFERNRSIIGRDVRMCH
                     PPGSVHVVEQILSDFKSGKENKAVFWMSNFMGRFIYIEYSAVRGKEGEYLGVVEVTQD
                     ITQM"
     gene            4900..5430
                     /locus_tag="PLI30_07070"
     CDS             4900..5430
                     /locus_tag="PLI30_07070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019537950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1858 domain-containing protein"
                     /protein_id="HOI79351.1"
                     /translation="MNTNKPDITPDTKVADLLKEFPQLEELLKHFSPAFAALKNPVLR
                     KTVAKVTSLQQAAKVGGVSIVEMVNTLRKEAGLLPLENSFCPNSEDIHIPFSEKMLPA
                     TITHTLDVRPIIESGNHPKDQILDLANKLNQGDCLEFISSFPPTPLIDLLQKKGYSVT
                     MLAPDEGVVRTFVKMK"
     gene            5531..6874
                     /locus_tag="PLI30_07075"
     CDS             5531..6874
                     /locus_tag="PLI30_07075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004299590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOI79352.1"
                     /translation="MTTRREFIKKAAIGTTLVSIGGVLPGFSARSYKNIVGANEKIKM
                     GAIGVNSRGNALSGGFAREKGCEIAYVCDVDRRAMEKCITNVKKIAGNTPQGEKDLRK
                     LLEIKDLDAVIIATPEHWHAPAALMAMKAGKNVYLEKPTSHNPAEDEILMQAVKKYNI
                     VTATGMQRRSWPNVIKAVQEIKEGIIGKVYFGKAWYANNRPSIGTGNVTAVPEWLDWE
                     LWQGPAPRVTSYKDNYLHYNWHWFYNWGTGEAGNNGVHFLDILRWGMDLTYPTHVDSS
                     GGRYLYSDDWEFPDTQIVNLEFGGNKLITWEGRSCNGRNIEDSSVGCAFYGESGTLVI
                     GGGNAYKVYDLKNKLVKEVTSEMSFKAGDTTNPTQQLDSFHFSNFLDGIRKGSTLNAD
                     INVGCISTTLAHLGNIAQRTRTTFETNPTNAHIINNRAASKLWSRKYQKGWEMKL"
BASE COUNT         2179 a         1494 c         1485 g         1797 t
ORIGIN      
        1 aattaggatc tgaacgctgt tggctgccaa cagcaagaat tactccgttg tttcttactg
       61 cttttaccaa ttcctggcct tcctttatag taaatgttaa cggtttttcg agatagacgt
      121 ttttcttggc attacatgct gctatggcaa taaacgcgtg ccagtgatcg ggcgtagcaa
      181 tcacaaccgc atcgatatcc tctcgtgcga tcaagtcctt atagttctca tacacctcta
      241 cggctacaga tttctgctgt gtgacattgt gatcgtttac gcgcttcaga aatcgttccc
      301 gcttgatacc gtacacgtcg catccggcca caacttctac ttccggcatc cccataaatc
      361 cgttcnnnnn nnnnnacatg gcttgctgcc ctaaaccaat aaatcccaga cggataattc
      421 catcggcaac ttcaccggct ttcttgttct gacaagacgt tacgaactgt gggaatacgg
      481 ctacaccaac agcaccgata gccgccgttt tcaaaaattt tcttcttgtt aattgattac
      541 tcatgacttt tagataaatt tttaagtata atttttttaa gtataacttt tttctttaaa
      601 ctttacaaat ataacggata aattcaaata taagtccatt tattgtggaa aaaatgcttt
      661 aataatgctc cagctttttc cggtttaaca cctttaattc ctttttgtat aaagaaataa
      721 caccatcatt gatcatctcg ccaatggtgc gtgcaaggga cggacgctga acaccaaaat
      781 attcggctaa ttgcgtctga gttcttttta aaataaaact gtcctgatcg ggagaggtgt
      841 tctcgaggat atataaggac aacttcgcac gcagactctt gatagaaagc attttgacct
      901 tccctgataa aaagaccgtc atattggaat tcagcctcaa aaagtttctc agcaacgttt
      961 cattcaacat catttttttg agaaaaacag attttggaat catataaaca actgccttct
     1021 ccgtggtaat ggcatcgaca ggaaacctgt gctggtcgga aaaaataaat gcaggtgcta
     1081 atggacgaac ggcctcaata aattcaattt caaccaaatt accctcagct gtaatcattt
     1141 cagtgcgcac aatcccttcc gtcaataagt acagaaaagt gatagtgtct ccctgtctaa
     1201 ccacgtattc tcctttctga aaagtccgga tttcacatac tgtatcggat ataaaatcct
     1261 caagagtttt atcgtcgatt ccggaaaaca gtggacaatc agcaagaatt gggttgtaca
     1321 tgtatttttt ttaaaaacaa aaataattaa atccatacaa acaatcaaaa aaacacgttt
     1381 atttactcaa gcagttaagt tccgcaggtt attcttccaa atggttgcat atgcaccctg
     1441 aaaatccaac aaactattta aaattaccta aaaaaatctc gtgtaacctg ggttacaaaa
     1501 gttcatgatt ctccggacta cctttgtgtc actaaacaat aacaataaaa attaaaagta
     1561 ttatggataa aatgttttgt tttcagtgtc aggaagcagc aaagaacgaa ggctgcaccg
     1621 taaagggagt atgtggaaaa accgcagatg tggccaacct tcaggatttg ctgttgttct
     1681 tgtgcaaagg aatttcacat tataccgttc cgttgagaaa atatgggatt gaaattccgc
     1741 agatcaacaa atttattacc gatagcttgt ttatgaccat cacaaatgcc aatttcgaca
     1801 aaaaccggtt taccacacgc ctgttaatgg ctttcgaaat gcggaatgcc gcaagggaac
     1861 gcctgactaa cacaggggga aatattgatg ggatcacttt tgacggtgct ctctgggtgg
     1921 gagaaaccga agccgaaata tcaaaaaaag cgttcgaagt gggtgtgctt accacaaaaa
     1981 atgaagatgt ccgctctctt cgtgaactca ccatttacgg attaaaagga atggctgcat
     2041 atgccgaaca cgctttcaac ttaggttaca cagaaaacga aattttcgct tttatgcagc
     2101 gtgcgctggt agcaacaacg gacgacaccc tttcggttga cgagttgacc gcactggttc
     2161 tcgaaaccgg gaaatacggt gtacaggtaa tggcattgct ggatagagca aacacatcga
     2221 gttacggaaa tccggaaatc acaaaagtaa accttggcgt acgtaacaat ccgggaattc
     2281 tcatcagcgg acacgactta aaggatatgg aagatctgct gaaacaaacc gaagggacag
     2341 gggtagatgt ttacacgcac agtgaaatgc ttccggcaaa ctactatccg gcatttaaga
     2401 aatacagcca cttcgtgggc aattacggga gttcatggtg gcatcaaacc gaagattttg
     2461 agaattttaa cggaattatc ctgtttacga ccaactgcat tgtccctccc cgaaaatcat
     2521 ccacttacat cgacagggtc tatacaacag gggcatcggg attcgaagga ttcaagcata
     2581 tcggggaccg aaaggaggga caaccaaaag acttttctgc aataatcgaa catgccaaaa
     2641 aatgtgctgc tcccaaagaa atcgaaacgg gtgagattgt aggcggattc gcacacaatc
     2701 aggtcattgc tcttgctgat aaagtggttg aagcggtgaa gtccggcgcc attcgcaaat
     2761 tctttgtgat gggaggatgc gacgggagaa tgaacggaag aaactattat accgagttcg
     2821 ctcaaaaact gcccggagat acagttatct taaccgccgg ttgtgccaaa taccggtaca
     2881 acaagttacc gttgggtgat atcaatggca ttccccgtgt acttgacgcc ggacagtgca
     2941 acgacagcta ctcactcgtg aaaatcgctc tcgcattaaa agaagcccta ggtttaaacg
     3001 acttgaacga tttgcccatc gcttataaca ttgcctggta tgaacaaaag gcggtaattg
     3061 ttttgctggc cctgctctat ttgggagtaa agaacatcca ccttggaccg accctccctg
     3121 cattcctttc acccaacgtg gtgaatgtac tggtaaacaa ctttggaatt acgggaatta
     3181 caagcgtgga cgaagatatg aaacttctcc tcgaaacggt ggagtgcaaa taacccggat
     3241 actcaaaaag ccaaaggtgt caaaaaagtt ctaaactgta actcaagtta caatttagtt
     3301 tcaatctaaa taatatcttt gcagtatcaa tcatcaataa aaaacaatta tggcaacttt
     3361 tgaacctgca aaaattttct cgatggaaaa ttcaattgaa tactcttcgg gaggcgtcgt
     3421 cagcaaacaa gttataaaaa agcaatcggg aaacgtcaca ttgttttctt tcgataaaga
     3481 ccagggactg acggaacaca cctcgcccta tgatgcgatg gtatacctgc tggacggtga
     3541 ggcagaaatc cgcatagacg gcaatcctca ccatttgaaa aaaggtgagt gcatcattat
     3601 gcctgccggg ttctctcatg cactctatgc tattgagccg tttaaaatgg tgttgacaat
     3661 gattaagcaa taaatttatg agtgaagtaa tcaacaattc gtcgcagcgc aaagaactgt
     3721 tgcgacactt tctcctccgt ttacacgaag gagaaaaccc cgaagtgctg cgacaaagac
     3781 ttatcgaagt gctccgcaac atcccataca acgaagtggt ggaagttgaa caggaactca
     3841 tcaacagcaa cgcacttacc gaggaagaaa ttctcgattt ctgcgatttg catacggctg
     3901 tactcgacgg tagcatcgat ttgggtggtg ccaaacccgt tcccgcaggg catccggtgg
     3961 acacattcaa aaaagaaaat cagtcattaa aaagagaagt aattaaaatc gccggcctgt
     4021 ttgataacgt aactgacctt agcgaaaaac aactgccggg atttatactg cagcttcgta
     4081 caatattcaa taacttatcc gatgtggata aacactacaa acggaaggaa tatctgcttt
     4141 tccccttttt ggagaaacac cttatcacag gccctccaaa ggtgatgtgg ggtaaacacg
     4201 acgaaatcag ggatttgttg aaaagttctt ttgaagcgct ggaatcaccc gtttcaacgg
     4261 tggaagaaat gaaatccctt gttcagctcg tacttgttcc ggccgttgag atgttggacg
     4321 gaatgattat gaaagaagag gaaattttgt tcccaatggc aatggacaca ttaaccgaag
     4381 aagaatggta caaggtctat caggaaacgc ctaatttcgg ttactgcctt tttgacccgg
     4441 aagacgaatg gcaaccgtcc ggcacaaagg ttgaaaaaca ggaatacgtg accgccgatg
     4501 ctatccgcct ttcctccgga gcatttaatc tcgctgagct agaagccctt ttcttacatc
     4561 ttcccatcga cattacattt gtggacaaaa tcgataaggt acgtttcttt tcacacagtc
     4621 ccaacagggt tttcgagcga aaccgttcta ttatcggacg tgatgtacgg atgtgccatc
     4681 cgccgggaag tgtgcacgtg gtagagcaaa tactttccga tttcaaaagc gggaaagaaa
     4741 acaaagccgt tttttggatg tcgaacttca tggggcggtt catctatatc gaatactcgg
     4801 ctgtacgcgg aaaagaaggg gagtatctgg gtgttgtgga agtaacgcaa gatatcacac
     4861 aaatgtgaaa actggaaggc gaccagcgac tgctttcgta tgaacacaaa taaacccgac
     4921 atcactcccg acaccaaagt tgccgattta ttaaaagaat ttccgcaact ggaagagctt
     4981 ttgaagcatt tctctcctgc ttttgccgca ttaaaaaatc cggtattgcg aaaaacggta
     5041 gcaaaagtaa cctcgttgca gcaagcggca aaagtgggag gtgtgagtat agtggagatg
     5101 gttaatacgc tcagaaagga agcaggacta ttgccgcttg agaacagttt ctgtccaaac
     5161 agtgaagata ttcatatccc tttctcagaa aaaatgctcc cggcaaccat tacgcacacg
     5221 ttggatgtcc gccccatcat cgaatccgga aatcacccta aggatcaaat acttgatctc
     5281 gccaataagt tgaatcaagg agattgtctg gaatttatct cttcgtttcc tcccacccca
     5341 ctgatcgatt tgctacaaaa gaaaggctac agcgtcacga tgcttgcgcc tgatgaaggc
     5401 gttgtaagaa ccttcgtgaa aatgaagtaa tctgcgtttt ttgatgataa tcaaacgtaa
     5461 atagaaaaac cgttgccaat taattttgta tttttgtcaa attcaaacgt gtttttctaa
     5521 aaatatcatc atgactacaa gaagagaatt tatcaaaaaa gcagccattg gcacaacact
     5581 ggtttctata gggggtgttt tacctggatt tagcgccaga agctacaaaa acatagtcgg
     5641 ggccaacgag aaaataaaaa tgggcgctat tggtgtaaac tcacgtggta acgccttgtc
     5701 gggaggtttt gcacgggaaa aaggatgcga aatagcttac gtttgcgacg tagacagacg
     5761 tgccatggaa aaatgtatca ccaacgtaaa aaaaattgca ggcaataccc ctcagggaga
     5821 aaaagaccta agaaagttac ttgaaataaa ggatttagat gctgtgatca ttgctacacc
     5881 cgaacattgg catgcaccag ctgctctgat ggctatgaaa gccggcaaaa atgtctatct
     5941 ggaaaaaccc accagtcaca atccggcaga ggatgaaatc ctcatgcagg cagtgaaaaa
     6001 atataacata gttactgcaa caggtatgca acgcagatcg tggcctaatg taatcaaagc
     6061 ggtacaggaa ataaaagagg gaatcattgg caaagtctac ttcggtaaag cctggtacgc
     6121 aaataacagg ccatcaattg gaacaggaaa cgttacggct gtaccggaat ggcttgactg
     6181 ggaactgtgg caaggtccgg caccaagggt aaccagctac aaagacaact atctccacta
     6241 taactggcat tggttctaca attggggtac cggcgaagct ggaaataatg gtgtgcattt
     6301 tcttgatatt ctccgttggg gaatggactt gacctatcct acccatgttg attcctcagg
     6361 aggcagatac ctttatagtg atgactggga atttccagat acacaaatcg tcaacctcga
     6421 atttggaggc aacaagctaa tcacatggga aggtcggagt tgcaacggtc ggaatattga
     6481 agactcttcg gtaggctgcg cattctacgg cgaatccggc accctggtga tcggaggagg
     6541 aaatgcttac aaggtgtacg acctgaagaa taagcttgta aaagaggtaa ccagtgaaat
     6601 gagtttcaag gccggggata ctacaaaccc cacccaacaa ttggactcgt tccatttttc
     6661 caactttttg gacggtatca gaaaagggtc tacgcttaat gccgatatta acgtaggatg
     6721 tatcagcaca accctggcac atttagggaa tatagcccaa cgcacaagaa ctacattcga
     6781 gacaaatccc accaacgccc acattatcaa taaccgtgca gccagcaaac tgtggagcag
     6841 gaaatatcaa aaaggttggg aaatgaaact ttaaattcat tcttataaat tctctcattc
     6901 tttcattctt tcattccgcc agatttttgt aaggttaaca cacaaaaatc ctggcggatt
     6961 ttttt
//
LOCUS       DAOSDR010000150         6931 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrimonas sp. isolate AMR_MDS_1826
            NODE_3983_length_6931_cov_3.921757, whole genome shotgun sequence.
ACCESSION   DAOSDR010000150 DAOSDR010000000
VERSION     DAOSDR010000150.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863355
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrimonas sp. (wastewater metagenome)
  ORGANISM  Petrimonas sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Dysgonomonadaceae; Petrimonas.
REFERENCE   1  (bases 1 to 6931)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6931)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:10:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,720
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 35
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6931
                     /organism="Petrimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3983_length_6931_cov_3.921757"
                     /isolate="AMR_MDS_1826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2023866"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1237)
                     /locus_tag="PLI30_07080"
     CDS             complement(<1..1237)
                     /locus_tag="PLI30_07080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006799524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DPP IV N-terminal domain-containing protein"
                     /protein_id="HOI79353.1"
                     /translation="MKKTLLFLYFVSSILLLNAQNYTLKDIVEGRFTAKDIRQMESSA
                     DGMHYYQMDPGNTAVIKFSYTTGDAVDTLFNTRRARECTFDTFQGFLVSPDENRVLVY
                     REKEQIYRHSFKATYYYHDVRRNLVRKLTQNPSKQMIPTFSPDGKMLAYVSDNNIWLA
                     KFDFDTESQVTKDGETNKVINGVTDWVYEEEFATTRLMEFSPDSKLLAFVRSDESQVR
                     QYQFQTFNQELYPGFYTYKYPKPGEKNSSVELRVFDIDARTTRKMDVPLDLDGYIPRI
                     TFTGNAEELVAMTLNRNQNRFDMFFVNPRTTVAKLILREENKYYVDAELLNGIHFLPN
                     RFTYVSEKEGYSHIYIYGYTGTLQKKLTTGPFDVTNLLAVDAQTQTVFYEAADESPLR
                     RNIYRVNIDKGQPQKLSVRQ"
     gene            1459..1869
                     /locus_tag="PLI30_07085"
     CDS             1459..1869
                     /locus_tag="PLI30_07085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019537851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SoxR reducing system RseC family protein"
                     /protein_id="HOI79354.1"
                     /translation="MIEHQGIITSISDKKISVKIIQQSACSTCHAKGACMAADSKEKV
                     VEVTDVTGKYKINDLVIIEGKESMGYKAVLWAFVIPVFILVLTIILATSFWKWGETEA
                     ATASILALVPYYLVLYFLRHKMANSFKFTIKNYH"
     gene            1880..2746
                     /locus_tag="PLI30_07090"
     CDS             1880..2746
                     /locus_tag="PLI30_07090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019537852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster domain-containing protein"
                     /protein_id="HOI79355.1"
                     /translation="MSVLLSAVATLGAIGAGSAAILYFVGRKFHVEEDPRIEEVQEAL
                     PAANCGGCGFPGCASFANACVKAETMDDLYCPVGGQETMDKVAEILGKTVATSAKKIA
                     VVRCSGSCDERPQLNLYDGSSNCAIAAALYGGDTGCSFGCLGLGDCEESCTFDAIHIN
                     PITRLPEVVEDKCTACGACVKACPKDIIELRKQGPKSRRIYVSCVNKDKGAVARKACN
                     VACIGCSKCQQACPFEAITIENNLAFIIDDKCRLCRKCVPVCPTNSILELNFPPRKET
                     PPAVKEEATVNA"
     gene            2802..4133
                     /gene="rsxC"
                     /locus_tag="PLI30_07095"
     CDS             2802..4133
                     /gene="rsxC"
                     /locus_tag="PLI30_07095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005641291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxC"
                     /protein_id="HOI79356.1"
                     /translation="MLKTFRIGGIHPKENKFSAGKAIQPIAIPAQVIIPLTQHIGAPC
                     QPVVKKGDKVKVGTLIGKTVGFVSANIHSSVSGTVLKIDKALDVSGYKRDAVFIQVEG
                     DQWEEKIDCSETLIKECTLSSKEIIDKISEAGVVGMGGATFPTHVKLIPPPGTKAETL
                     IVNAVECEPYLTSDHQLMMERTEEILVGTTILMKAINVDKAVIGIENNKKDAISKFTT
                     TAKSYPGITIQPLKVQYPQGGEKQLIDAVVRRQVPSGALPISVGVVVQNVGTAFAVYE
                     AVQKNKPLVERIVTVTGKDVKNPCNVLARVGIPVNNLIDIAGGLPETTGKIVCGGPMM
                     GKAIASVEIPVTKGTSGILIIPTFESKRDRMKDCIRCTKCIHVCPMGLNPTLLMTLTE
                     YSEWDRAEKGRITDCIECGSCSYTCPSDRPLLDYIRLGKGKVMGNIRARKN"
     gene            4228..5193
                     /locus_tag="PLI30_07100"
     CDS             4228..5193
                     /locus_tag="PLI30_07100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019537854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit D"
                     /protein_id="HOI79357.1"
                     /translation="MNNKLIVSPSPHVHSGDSIEKNMYGVLIALIPAFLVAIYVFRLD
                     ALIITALSVLFCVGFEYLIARFILKTEPSVFDGSAIITGVLLAFNVPSNLPVWILALG
                     ALFSIGVVKMSFGGLGNNIFNPAIAGRIFLLISFPAQMTTWPTPSVGSTTDAVTSATV
                     LSNLRFNPDSLPAIKDMFLGFEGGSIGEMSALALLLGLAYLLWKKIITWHIPVSIILS
                     VALFTGILFIFNPIPMFNPLIHLFSGGLMLGAIFMATDYVTSPMTKSGMLIYGVGIGL
                     ITVSIRLWGAYPEGVSFAILLMNAFTPLINNYTTPKRFGEVVKNG"
     gene            5186..5842
                     /locus_tag="PLI30_07105"
     CDS             5186..5842
                     /locus_tag="PLI30_07105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018711562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit G"
                     /protein_id="HOI79358.1"
                     /translation="MAKLKSTFKNMFLSLSIISLVVAVLLAQVNKMTAKPIAAAKALK
                     LENAIKEVVPEFDNNPVEEAYKMATPNGDSLLVYPAKKGDEVVGFAINTYSNNGFSGN
                     IQLMVGFDTKDRVVNYAVLSHAETPGLGSKMNDWFRDTARPNQVVVGRNLSEGNLSVS
                     KDGGQIDAITASTITSRAFLEAINRAYMVYKKSDVDSSATKQNKPDSNQEEEGGESHE
                     "
     gene            5835..6422
                     /locus_tag="PLI30_07110"
     CDS             5835..6422
                     /locus_tag="PLI30_07110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019537856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit E"
                     /protein_id="HOI79359.1"
                     /translation="MNNNLKVIVNGLIKENPTFVLLLGMCPTLATTSSAINGMSMGLA
                     TMFVLICSNAAISALKNLIPDMVRIPAFIVVIAAFVTMVEMVMNAYVPALAASLGIFI
                     PLIVVNCIVLGRAEAFASKNNVLASSLDGFGIGLGFTLALTLLGAIRELLGTGKVFSL
                     SIYPENFGSLIFVLAPGAFIVLGFLIAAFNKLQKK"
     gene            6483..>6931
                     /locus_tag="PLI30_07115"
     CDS             6483..>6931
                     /locus_tag="PLI30_07115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002562594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rnf-Nqr domain containing protein"
                     /protein_id="HOI79360.1"
                     /translation="MEYIGIIIVAIFVNNIVYSQFLGICPFLGVSKKIDTAIGMGLAV
                     TFVLTISTIVTFLLQKGILDPFGLGYLQTISFILVIAALVQMVEIILKKVSPSLYQAL
                     GVFLPLITTNCAILGVAILVIQKDFNLLQSVVFAIATSLGFALALII"
BASE COUNT         1925 a         1483 c         1602 g         1921 t
ORIGIN      
        1 cctgccgcac ggatagcttt tgcggttgac ccttatcaat atttaccctg tagatattcc
       61 ggcgaagggg actttcatcg gcagcctcat agaaaaccgt ttgggtctgc gcgtccaccg
      121 ccaggagatt tgtcacatcg aaagggcccg tagtcagctt tttttgcaac gttcccgtgt
      181 aaccgtaaat ataaatatgg ctgtatccct ctttttccga cacgtatgta aaacggttgg
      241 gtaagaaatg gattccgttg agcagttcag catcaacgta atatttgttt tcttccctaa
      301 gaatgagttt agcaactgtt gttcttggat ttacaaaaaa catatcaaat cggttctgat
      361 tgcggttcag ggtcatagcc accagttctt cggcatttcc ggtgaaagtt atccgcggta
      421 tataaccgtc tagatccaaa gggacatcca ttttacgggt cgtacgggca tcgatatcaa
      481 aaacacgcaa ttctaccgat gagtttttct cgcccggctt ggggtatttg taggtataaa
      541 acccgggata aagttcctgg ttaaaagttt gaaactgata ttgacggact tgcgactcat
      601 ctgaacgaac aaaagccaat aacttgctat cgggtgaaaa ttccattaac cgggtggtag
      661 caaattcttc ttcgtaaacc caatcggtga ctccgttaat aactttgttg gtttcaccgt
      721 ctttggtaac ctgagattct gtatcaaaat cgaatttggc aagccagata ttgttgtcgc
      781 tcacgtaagc cagcattttc ccgtcgggag aaaacgtagg gatcatctgt ttggaaggat
      841 tctgcgtaag tttacgtacc aggttgcgac ggacgtcgtg atagtaataa gttgccttga
      901 acgagtggcg gtagatctgt tccttttctc tgtaaaccaa caccctgttt tcatccggac
      961 tcaccagaaa tccttgaaat gtgtcaaaag tacattctcg tgcccgacgc gtgttgaaaa
     1021 gcgtatccac cgcatctccg gtagtatagg aaaacttaat aaccgccgtg tttcccgggt
     1081 ccatctggta gtaatgcatc ccatcggccg aagattccat ctgccggata tcttttgccg
     1141 taaatcggcc ttcgacgatg tcttttaatg tgtaattttg tgcatttaac agcaaaatgc
     1201 tgcttacgaa atagaggaat aaaagtgtct ttttcattat tatatgtttt gttcttttac
     1261 cgtttaattg ctctagcctg cgaataaaat ccgtcaatag atgactttat cgatcacaaa
     1321 aataagaatt atatccgaca tttttctgaa ggagaaaata tctcaatcgt taaataacag
     1381 aaacaagcaa caaaatcaca aaaattatac ctaattttgc agcgtcattc tggaaataga
     1441 tgacagaaag tcatttttat gattgaacac cagggaatca taacaagtat atcggacaag
     1501 aaaatatccg ttaaaatcat acaacaatcg gcttgcagta cctgtcatgc gaaaggagca
     1561 tgtatggcgg cagactcgaa ggagaaagtg gtagaagtga cggatgttac ggggaaatat
     1621 aagataaatg accttgtaat tattgaggga aaagaatcaa tgggctataa agcggttttg
     1681 tgggcttttg ttattcctgt ttttattctt gttctgacaa taatacttgc cacctctttt
     1741 tggaagtggg gagaaacgga agccgctacc gcttccatcc ttgcattggt gccctattac
     1801 ctggtacttt attttttacg tcataaaatg gccaattctt ttaaatttac tatcaaaaac
     1861 taccattgat acttagaata tgagtgtatt actttctgct gtagccacat taggagcaat
     1921 cggagccgga agcgccgcca ttctctattt tgtgggaaga aaatttcacg tggaagagga
     1981 tccccgtatc gaagaggtgc aggaagcgct tcctgcagcc aattgcgggg gatgcggctt
     2041 cccgggatgt gcctcgtttg ccaatgcttg cgtgaaggcc gaaaccatgg acgatctgta
     2101 ctgccctgtt ggcggacaag aaacaatgga caaggttgcc gaaatattgg gaaaaacggt
     2161 tgccacctcc gcaaaaaaga ttgcggttgt caggtgtagc ggttcgtgcg atgaacgtcc
     2221 gcagttgaat ttatacgatg ggtcttccaa ctgcgccatt gctgcagcgc tttacggtgg
     2281 agacaccggc tgctcgttcg gatgcctggg attaggcgat tgcgaagaat cgtgcacgtt
     2341 tgatgccatc catatcaatc ctataacccg gctgcccgaa gtggtggagg acaaatgtac
     2401 tgcctgcgga gcttgcgtta aagcctgccc gaaagacatt atcgagttgc ggaagcaggg
     2461 ccctaaatcg cgccgtattt atgtcagttg cgtaaacaaa gacaaaggcg ctgttgccag
     2521 gaaagcttgt aatgtagctt gtatcggttg ctccaaatgc caacaggctt gtccgtttga
     2581 agccattacg atcgaaaata acctggcgtt catcatcgat gacaaatgtc gtttatgccg
     2641 taagtgcgtt ccggtttgtc caacaaactc gatattggag ctcaacttcc cgccgaggaa
     2701 agaaacacct cctgctgtta aggaagaagc gacggtaaac gcttaattca aataaaaaac
     2761 tcatcccaaa aaagttctca tcaaggagat ccaggagttc tatgttaaaa acatttcgta
     2821 tcgggggaat ccaccctaaa gaaaataaat tttccgccgg aaaagcaatt caacccattg
     2881 ccatacctgc acaagttatt attccgctta cccagcacat tggcgctccg tgccagcctg
     2941 tggttaaaaa aggcgacaaa gtgaaagtag ggacccttat cggaaaaacg gtagggtttg
     3001 tatctgcaaa cattcactcg tctgtttcgg ggactgtact taaaattgat aaagctcttg
     3061 acgtcagcgg atataaacgc gacgccgtgt ttattcaagt tgaaggtgat cagtgggagg
     3121 aaaagattga ttgttctgaa actctgataa aagaatgtac actttcttct aaggaaatta
     3181 tcgacaagat ttccgaggct ggtgtagtgg gtatgggagg tgccactttt cccactcacg
     3241 taaaactgat tccaccacca ggaacaaaag ccgaaacact tatcgttaac gccgttgaat
     3301 gtgagcctta tctcacatcc gatcatcagt tgatgatgga gagaaccgaa gaaatactgg
     3361 taggaacaac catactgatg aaagccatca acgtggacaa agcggtcatc ggtatcgaga
     3421 acaacaaaaa agacgctatc tccaaattca ctactacagc caaatcgtac ccgggaatta
     3481 ccatacaacc gttaaaggta caatatccac aaggcggtga aaaacagctg atcgatgccg
     3541 ttgttcgtcg tcaggtccct agcggcgcgc ttcccatttc ggtaggcgtg gtcgtgcaaa
     3601 acgtgggaac agcttttgcc gtttacgaag ctgtccagaa aaacaaaccg ctcgtggagc
     3661 gcatagtcac cgttaccgga aaagatgtca aaaacccgtg caacgtgctt gcccgggtgg
     3721 gaattcccgt aaacaacctg atcgatatag caggagggtt gcccgaaaca accggaaaaa
     3781 tagtgtgtgg cggccctatg atgggaaaag ccattgcgag tgtggaaatt cctgtaacca
     3841 aaggtacctc gggaatactg attatcccca cgttcgagtc gaaacgagac aggatgaaag
     3901 actgtatccg ttgcaccaag tgcatacatg tatgcccgat gggtttaaat ccgacacttt
     3961 taatgactct gacagaatac agtgaatggg acagggccga aaaagggcgc ataaccgatt
     4021 gtatcgaatg cggctcttgc agctatacct gtccgtccga ccgtcctctg ctcgactata
     4081 tccgcctggg aaaaggcaag gtgatgggga acatcagagc aagaaaaaac tgatttcggt
     4141 tttgtgattt gagaacggca aaaattagaa ctaaatacta acaaataaat aatatttcaa
     4201 ttccctttcc ggggattcag gggtaatatg aacaacaaac taatcgtctc cccttcaccg
     4261 catgtgcaca gtggagacag tatcgaaaaa aacatgtacg gcgtactgat cgcacttatc
     4321 ccggcgtttc tggtcgcaat atatgtgttc cgactggatg ctttgataat cacggctctg
     4381 tcggtattat tctgtgtggg atttgaatac ctcattgcca ggttcatcct gaaaacggag
     4441 ccttcggttt tcgatggttc tgccataatc accggtgtat tactggcatt caacgtaccg
     4501 tccaacctac cggtgtggat tttggcactt ggggcacttt tttccatcgg tgtggtaaag
     4561 atgtcgttcg gcggattggg aaataatatc tttaatcctg ctattgcagg gcgtatcttc
     4621 ctgttgatct cctttccggc acaaatgacc acgtggccta cacctagtgt tggctcaaca
     4681 acggatgcag taacatcggc aaccgtttta tcaaacctga gattcaatcc cgattcattg
     4741 cctgcaataa aggatatgtt cctggggttt gaaggtgggt ccatcgggga aatgagcgct
     4801 ttggctcttt tgttgggatt ggcgtatctg ttgtggaaaa aaatcatcac ttggcacatc
     4861 ccggtatcga ttatcctgtc ggtagccctg tttacgggaa tcttgtttat ctttaatcct
     4921 attccgatgt tcaacccgct tatccacttg ttctcaggag ggttaatgct gggagccatc
     4981 tttatggcaa ccgattatgt aacgtcgcca atgacaaagt cggggatgct catttatggc
     5041 gtaggcatcg gattgataac tgtttctatc cgattgtggg gtgcttaccc ggaaggggtt
     5101 tcatttgcca tcttgctgat gaacgctttt actccgctga tcaataatta cacaacacca
     5161 aaaagattcg gggaggtagt gaaaaatggc taaattaaaa tctacattca aaaatatgtt
     5221 cctttcgtta tccataatca gtttagtggt agcggtttta ctggcgcaag tgaacaaaat
     5281 gactgccaaa cccatagcag cagccaaggc attaaaatta gaaaacgcaa taaaagaagt
     5341 ggttcccgaa ttcgacaata atccggttga agaagcctac aaaatggcga cacccaatgg
     5401 cgactcgtta ttggtttatc ctgccaaaaa aggtgacgag gttgtcggtt ttgctataaa
     5461 cacttattct aacaacggat tcagcggaaa tatacagtta atggtgggtt ttgacaccaa
     5521 agacagggta gtcaattacg ctgttctttc gcacgccgaa actccgggac tgggatcaaa
     5581 gatgaatgac tggtttcgcg atacagccag gcccaatcag gttgtggttg gcagaaacct
     5641 gtcggaagga aatttaagtg tttcaaaaga cggcggacaa atcgatgcca tcaccgcatc
     5701 caccatcacc tcgcgtgctt tcctggaagc cataaacaga gcatatatgg tttataagaa
     5761 aagtgatgtt gattcaagtg ccacaaaaca aaacaagcct gacagcaacc aggaagagga
     5821 aggaggagag agtcatgaat aacaacttaa aagtcattgt aaacgggctt attaaagaga
     5881 accccacgtt tgtgcttttg ttgggaatgt gccctaccct ggcaacaacc agttcagcaa
     5941 tcaacggaat gagtatggga ttggcaacca tgtttgtgtt gatttgttcc aatgcagcca
     6001 tctctgcatt aaaaaacctt attcccgata tggtgcgcat tccggcattc attgtggtaa
     6061 ttgccgcttt cgtaacaatg gtggagatgg tgatgaacgc ctacgttcca gccttagctg
     6121 cgagcctggg gattttcatt ccacttattg tggtaaactg tatagtgcta ggacgtgctg
     6181 aagcgtttgc gtccaaaaac aacgtcttgg catcgtctct cgacggtttc ggtattggtc
     6241 tgggatttac cctggcgctc acattgttgg gcgctattcg tgaattactg ggaaccggga
     6301 aagtattttc cttatccatc tatccggaaa attttggttc actcatcttt gtgcttgcac
     6361 cgggtgcgtt tatcgtactg ggatttctga tcgcggcgtt taataagttg cagaaaaagt
     6421 agaactgggt tgcctattga aaaatttcgc tgagaacgcg aaactcaaaa caaaagaaga
     6481 ttatggaata catcggaatt ataatagttg ctatttttgt aaacaatatt gtttactcgc
     6541 aatttttggg aatctgccct ttcctgggtg tgtctaaaaa aatagatacc gccatcggca
     6601 tgggccttgc cgttactttt gtactgacca tttccaccat tgtcactttc ttgctgcaaa
     6661 aagggattct cgatcctttc ggcctgggat acctgcaaac catctctttt attttggtta
     6721 ttgctgcttt ggtacagatg gttgaaatta tattgaaaaa ggtttcgcca tcattgtacc
     6781 aggcgttagg ggtttttctg ccgttaatca ccaccaactg tgctattctg ggtgttgcaa
     6841 ttttggttat tcaaaaagat ttcaacctgc tgcaatcggt agtatttgcc attgctactt
     6901 cgctcggttt tgcactggcc ctgattattt t
//
LOCUS       DAOSDR010000151         6879 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrimonas sp. isolate AMR_MDS_1826
            NODE_4012_length_6879_cov_3.755129, whole genome shotgun sequence.
ACCESSION   DAOSDR010000151 DAOSDR010000000
VERSION     DAOSDR010000151.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863355
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrimonas sp. (wastewater metagenome)
  ORGANISM  Petrimonas sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Dysgonomonadaceae; Petrimonas.
REFERENCE   1  (bases 1 to 6879)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6879)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:10:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,720
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 35
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6879
                     /organism="Petrimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4012_length_6879_cov_3.755129"
                     /isolate="AMR_MDS_1826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2023866"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..771)
                     /locus_tag="PLI30_07120"
     CDS             complement(<1..771)
                     /locus_tag="PLI30_07120"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HOI79361.1"
                     /translation="MEKRLIMVFISLLLSAFLVNAQEINLSGKVTQSDGNSVPGVSVA
                     IKGTSLGTVTNIDGQFSLKSPVGSKTLVFSFVGMKTKEVEITSSLVYNVVLEEDVYNL
                     EEVVAIGYGTMKKSDLTGSVTSVESKDLTAYPATNVMKAMAGRAPGVFVQQNNSAPGG
                     GIQVRIRGTNSISGDNNPLYVIDGFLYNGTPLILQPNDIESMEILKDASATAIYGSRA
                     ANGVVLITTKKGKVGKTKVEFESGYTIQSPSKKMDLMNA"
     gene            1050..2279
                     /locus_tag="PLI30_07125"
     CDS             1050..2279
                     /locus_tag="PLI30_07125"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOI79362.1"
                     /translation="MKIRKTPTLLTYKWEMILLLWLAFFFNQADRQIFNVVLPAIRSD
                     LNLADSDMGLVASILILVYGILVPVAGYVGDRSNKKYVLMFSLLIWSTSTLFSGMSTM
                     LIHLIFLQSIATGGGEAFYSPSANAMIGEHHDKTRATAMSIHQTALYAGIIMSGYLAG
                     WIADQFGWRVAFYVFGGLGIILSLIIAARLKDSLPVNTAGNKVRLSDALNMFFRKPTA
                     LLLTGAFAGMVFVNVGFMTWMPTYLHEKFGFSLARSGFDATFYHYVAAFIGVLLGASL
                     ADRFSNKVTGMRGIVQGTGLLLGAPFIFWLGIGNTPFLVYLALAFFGIFRGVYDSNIF
                     AALYDVVEPRFRSTATGLMLMFAFITGASAPYILGKIKPILGLAWGLSALSVVYIAAA
                     LCIFIAVRFYYKKDIIK"
     gene            2285..3037
                     /locus_tag="PLI30_07130"
     CDS             2285..3037
                     /locus_tag="PLI30_07130"
                     /inference="COORDINATES: protein motif:HMM:NF013757.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOI79363.1"
                     /translation="MIQVINRALDILELIGQDRTRLYTLSEISRQLKLNNATCANIIK
                     TLVNRGYLEQEGRKTGYRLGSMSYLLTGNYSHKQELLSAAIEPMNQLSATLNEGCILA
                     VLKDNIRIILSEVKSTHELQVINKKEKDAYRTSTGRMILACMDEKEQLDFIHKFGLPS
                     PDIWAGIEDEEDLIMELCKIRKKQLAQQISVAQIIGLAIPVFKDKKVIASLGIYLPQT
                     RYTPAMQDKILNNLKETGELINKNLNEKNTEH"
     gene            3048..4238
                     /locus_tag="PLI30_07135"
     CDS             3048..4238
                     /locus_tag="PLI30_07135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008152779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
                     /protein_id="HOI79364.1"
                     /translation="MKIIDLKARTVAIPLNAQLRHNTGVHPGYLLRTVIEIITDEGIV
                     GLGEVGGGDQRAAFAKLKPRILGLDPFHLETIKQKVLRSIYYMSNARLYAAIEIACLD
                     IQGKVLNRPLSDLLGGKLKNEVPFIAYLFWRYDRPGGGHDKYAEDMVDLCTQLHEELG
                     VSSMKLKAGVMKPREEVRVLELCRKNLGEDFGLRIDPNGVWSVTTAVQMGKIMEDLNP
                     EYFEDPSWGLEGNATVRKQIRIPIATNMYPNKFDDLGPSIRMGAVDIVLTDLHYWEGP
                     KGVKDLVAVCRTFNLGVAMHSGAEFGIELAAMIHTASTIPTMTFAGDAHYHYLTDDII
                     KGGLMKYESGCIKVPEGPGLGVELDEEKMEKYERYYNEKGDYYARFHQDEYRPDWFPI
                     VGSI"
     gene            4248..5072
                     /locus_tag="PLI30_07140"
     CDS             4248..5072
                     /locus_tag="PLI30_07140"
                     /inference="COORDINATES: protein motif:HMM:NF020039.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HOI79365.1"
                     /translation="MIYQSIISGLNFPEGPVFDFEGNLWFVEIKGGNLSKWDGEKLTR
                     FDVDGTPNGAMIDQSGCIWFCDSGRGEIRKFDPVHQTFLTACNDTIEGNRLKRPNDLI
                     FDKAGNLLFSDHADGREEPLSTICVLPNGKSRAKVISANKYFTNGLALGSDARTLIFA
                     ETYRQKLWIAEWDSTLLELWNERPFAKAGDGPWGPDGITFDEFENLYAAIFNEGIINI
                     YNKTGQQTESIACPGKRPTSCAFDPFGKLGLVVTEADLGQILSYKIKNRGLTLYYR"
     gene            5074..5847
                     /locus_tag="PLI30_07145"
     CDS             5074..5847
                     /locus_tag="PLI30_07145"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008152778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOI79366.1"
                     /translation="MELKKLFNLNEKTALVTGGSQGIGKAISLALAEFGANLIINYRS
                     GKELAEETCREVTNRGVNCHLIASDLSDVNSSKIISEFVTKNKLEVDILILNASVQFR
                     KSWDQVTPEEFETQINTNLRASLFLIQEFYPQMQKKQWGRIVTIGSVQQIRPHQQMIA
                     YAASKVAQVSMVKSLAPQFGPSGVTINNLAPGAILTGRNVEVLKDDGYKKSVEARIPL
                     GFIGEPVDCAATALLLCSDAGRYITGQDLYVDGGMGLNF"
     gene            5881..6624
                     /locus_tag="PLI30_07150"
     CDS             5881..6624
                     /locus_tag="PLI30_07150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008152777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RraA family protein"
                     /protein_id="HOI79367.1"
                     /translation="MSVDQYKKEVGMMNLERLIAEREGISKTSFPIPDEELLYRFEKL
                     YTGAVNDVLREFCLLEQALPKRIIPLREYRSVAGFAFTVKSSPNVKIQGEMEFRAQML
                     DEMHKDAFVIWDTSRDEKATLWGGVMTATAKSKKVKAACIDGGIRDTHQILEADFPVF
                     YEYRISNGSLGRCLITHYQVPVKIGDVIVKPGDVILGDIDGVLVVPRNIAFDILLRAE
                     EIKENEKRIFEWVREGQSVHDITEKGGYF"
BASE COUNT         2115 a         1358 c         1490 g         1916 t
ORIGIN      
        1 tgcattcatt aaatccattt tcttgctggg actttgtatg gtatatccag actcaaattc
       61 cactttagtt tttcctacct tacctttttt tgttgtaata aggacgactc cgttagcagc
      121 cctcgaaccg taaatagcag tggcagaagc atccttcaaa atttccattg attcaatatc
      181 atttggttga agaattaaag gagttccgtt atacaaaaaa ccgtcaatta catataatgg
      241 attattatcg ccggaaatag agttagtgcc gcgaattctt acctgaatgc ctcctcccgg
      301 agcagagtta ttttgctgta caaacacacc gggagcacgc ccggccattg ctttcataac
      361 attcgtggca ggatatgctg ttaagtcttt tgactcaacc gatgtaaccg accctgttaa
      421 atcactcttt ttcattgtac cataaccaat cgccacgact tcttccagat tataaacatc
      481 ttcctccaaa acaacattat acactaaaga agaggtaatt tcaacttctt tcgttttcat
      541 tccaacgaaa ctaaaaacca atgtttttga acctacaggt gacttcaatg aaaattgtcc
      601 gtcaatattc gtaacagtac caagagaagt acctttaata gcaacactta ctccaggcac
      661 agaatttccg tcactctgag taactttccc ggacaaatta atttcttgag catttaccaa
      721 aaaggcggaa agcagcaaac tgataaatac cattattaat cgtttttcca taagtaaatt
      781 atttaagatt taattttcag atacaaaata aaattaaaca aaccaaagca tcattatatt
      841 ataaaatgtt tttaaacatt attttatatt ataaaattaa aaatacatgt cattgcagcc
      901 cgttttaccc aaaaaactaa tatacaatat tttacaaaaa aaaacaaact atatttaaaa
      961 aaaaaacggc tcaacaactg aatgacaaaa atatacatgt tgttttttta caataacaaa
     1021 tatagatttg cattgtaaat caatataaaa tgaaaattcg aaaaacaccg actttgttaa
     1081 cctataaatg ggaaatgatt ctgctgttat ggctggcttt cttttttaac caggcagatc
     1141 gccagatttt caatgtcgtt ttaccggcta tccgctcaga cttgaacctt gccgattctg
     1201 atatgggttt ggtcgcttcc attctaatac tggtttatgg tattcttgtc cctgttgccg
     1261 ggtatgtagg ggatcgttcg aataaaaaat atgtattgat gtttagcctg ttgatttgga
     1321 gtacgtcgac cctgttttct ggtatgtcaa ctatgttgat tcatcttatc tttcttcaaa
     1381 gcattgccac cggaggagga gaagcctttt attctccgtc ggcaaatgca atgattggcg
     1441 agcatcatga taaaacaaga gccacggcta tgtcgattca ccaaacagct ttatatgcgg
     1501 ggattattat gagcggatat ctggctggat ggattgctga ccaatttgga tggagagttg
     1561 cattttatgt gtttggagga ttgggcatca tcttgtcgtt aattattgcg gcgcggttaa
     1621 aagactccct gccggtgaac acagccggta ataaagtccg tttatctgac gctttgaata
     1681 tgtttttcag gaaaccaacc gcccttttgc ttaccggggc atttgccggg atggtttttg
     1741 tcaatgtagg ttttatgacc tggatgccga cttaccttca cgaaaagttt ggattttcat
     1801 tagcgcgttc agggttcgac gcaacatttt accattatgt tgccgcgttt attggcgttc
     1861 tgttaggcgc ctcattagcc gatcgtttca gcaacaaagt tacagggatg cgaggaattg
     1921 tgcaggggac aggattattg cttggcgcac cttttatttt ttggctaggt attggaaaca
     1981 cgccttttct tgtctacttg gctcttgctt tttttggcat attcagagga gtttacgact
     2041 ccaatatttt tgctgcgcta tacgacgtag ttgaacccag gtttcgctcc acggcaaccg
     2101 gcttgatgct gatgtttgca tttattacag gggcatctgc tccttatata cttgggaaaa
     2161 taaaacccat tctggggctg gcgtggggac tgtctgccct gtcggtagtt tatattgctg
     2221 cagcgttatg tatttttatc gctgtaagat tttattacaa aaaagatata atcaaataaa
     2281 gaatatgatt caggtaatta acagagcatt agacattctg gaactcattg gccaggaccg
     2341 gacacggtta tatacactca gcgaaatatc gaggcagtta aaactaaata atgcgacctg
     2401 cgccaacatc attaaaacgt tggtgaaccg ggggtacctc gaacaggaag gaagaaaaac
     2461 cggatatcgg ttaggatcta tgtcgtatct gctaacggga aattattcac acaagcagga
     2521 acttttatcg gccgcaatcg aaccaatgaa ccagctctca gctacattaa acgaaggttg
     2581 cattctggct gtacttaaag ataatattcg tataatatta tccgaagtca agtccactca
     2641 tgaacttcag gtcatcaata aaaaagagaa agatgcttac aggacttcta ccggccggat
     2701 gattttggct tgtatggacg aaaaggaaca attggatttc atccacaagt ttggactccc
     2761 ttctcctgac atttgggcag ggattgaaga tgaagaggat ctgataatgg aactttgtaa
     2821 aataagaaaa aagcaactgg cacagcagat ttcagtagct caaatcatcg ggttggccat
     2881 tcccgttttt aaagataaaa aagtgatcgc aagcctcggg atttatttac ctcaaacaag
     2941 gtacactccg gcaatgcagg acaaaattct taataattta aaagaaacag gggaactaat
     3001 taataaaaat ctgaacgaaa aaaatactga acattaattt ttaaaacatg aaaataattg
     3061 atttgaaagc ccgcaccgtt gcgattccac ttaacgcaca actaaggcac aataccggcg
     3121 ttcatcccgg atacttactc cgaaccgtca tcgaaattat aacagacgaa ggaattgtcg
     3181 gattaggtga agttggcggc ggcgatcagc gtgccgcctt tgcaaagctg aaacctcgga
     3241 ttctcgggct cgatcctttc cacttagaaa caataaagca aaaagttttg cgaagtattt
     3301 actatatgtc gaatgcaagg ctttatgctg ccattgaaat tgcctgtctc gatatccagg
     3361 gaaaagtatt gaaccggccg ttgagtgatc tcctcggagg caagctaaaa aacgaagtcc
     3421 cgtttattgc ctacttgttc tggcgatacg accgcccggg cggcggacac gacaaatatg
     3481 ccgaagacat ggttgattta tgcacacagc tgcatgaaga gttgggcgtc agctccatga
     3541 aactaaaagc gggagtgatg aaaccccggg aggaagtcag ggtattggaa ttgtgccgga
     3601 aaaacctggg tgaagatttt gggctccgca tcgatccaaa cggagtttgg tcggttacta
     3661 ctgccgtgca aatgggaaaa attatggaag accttaatcc ggaatacttt gaagatcctt
     3721 cctggggtct ggaagggaat gctaccgttc ggaaacaaat ccgcattccc attgcaacca
     3781 acatgtaccc caataaattc gatgatcttg ggccgtccat ccggatgggg gcagttgaca
     3841 ttgtgcttac cgatctccac tactgggaag gccccaaagg ggtgaaagat ttggtagcgg
     3901 tttgccgtac cttcaacctc ggagtagcca tgcacagcgg cgctgagttt ggaattgaac
     3961 tggctgccat gatacacacc gcttcgacca ttcctaccat gacttttgca ggtgacgctc
     4021 actaccacta tttaaccgac gacatcatca aaggtggatt aatgaaatat gaaagtgggt
     4081 gtataaaagt acccgaaggc ccgggattag gggttgaact ggacgaggaa aaaatggaaa
     4141 aatatgaacg ttactacaac gaaaaaggag attattatgc ccggtttcac caggatgaat
     4201 accggccgga ctggttcccc attgttggaa gtatctaata gcaggaaatg atttaccaat
     4261 caattatatc aggtctgaat tttccggaag gtcccgtctt cgattttgaa ggcaatcttt
     4321 ggtttgtaga aatcaaaggc gggaaccttt ctaaatggga cggggaaaaa ctgacccgtt
     4381 tcgatgtgga cggaacgcca aacggggcaa tgattgacca aagcggttgc atttggtttt
     4441 gtgatagcgg acggggagaa atcagaaaat tcgatcctgt tcaccaaact tttctaacgg
     4501 cctgcaatga tacaattgaa ggtaatcggc tgaaacgccc caacgatttg atctttgaca
     4561 aggcaggtaa cctgcttttc tccgaccacg ccgacggccg cgaagaaccg ctttcaacca
     4621 tttgcgtatt gcccaatgga aaaagcaggg caaaagtaat ctcagcaaat aaatatttta
     4681 caaatggatt ggcccttggc agcgatgcca ggactcttat attcgctgaa acctaccggc
     4741 aaaaactttg gattgccgag tgggattcaa ctttgctcga actttggaat gaacgccctt
     4801 ttgcgaaagc cggagatggc ccatggggcc cggacggaat tacatttgac gaattcgaaa
     4861 atttgtatgc cgccattttt aatgaaggta ttattaatat ttacaataaa acaggacaac
     4921 aaaccgaatc catagcatgt ccgggaaaaa gaccgaccag ttgtgccttc gacccgtttg
     4981 gaaaacttgg gctggttgta acagaggcag atctgggaca aattctttca tacaaaataa
     5041 aaaacagggg attaacactt tattacagat aatatggaac taaaaaaatt attcaacctg
     5101 aatgaaaaaa cggccctggt taccggtgga agccagggca ttggaaaggc catctcgctg
     5161 gcattggcag aatttggggc caatctgatc atcaactacc gctctggaaa agaacttgct
     5221 gaagaaacct gccgtgaggt aaccaaccgg ggagtcaact gtcaccttat cgccagcgat
     5281 ctctccgatg tcaattcttc aaaaatcatc tcagaatttg taaccaaaaa caaactcgag
     5341 gtagatatac tgatattaaa tgcatcggta cagtttcgta agtcctggga tcaagtaaca
     5401 ccagaggaat ttgaaactca aatcaacaca aatcttcggg catcgctttt tcttattcag
     5461 gaattttatc ctcaaatgca aaaaaaacaa tggggacgta ttgttactat cgggagtgtt
     5521 cagcaaataa ggcctcatca acagatgatt gcttatgctg cttcgaaggt ggcacaggtg
     5581 agtatggtaa aaagcctggc cccgcaattc ggcccttctg gagtgactat taataacctg
     5641 gcacccggag ccattctcac cggaagaaac gtagaggttc tgaaagatga tggttacaaa
     5701 aaaagcgttg aggcaagaat cccgcttgga tttatcggag aacccgttga ctgcgctgcg
     5761 actgccctgc ttttatgttc cgatgccggg cgttatatta ccggtcaaga tttatatgtt
     5821 gacggaggga tgggactgaa tttttaaaaa agaaatacgt aaattttaat atcttgaact
     5881 atgagtgttg atcagtataa aaaagaagtg ggaatgatga acctagagag attaattgct
     5941 gaaagagaag gaatatcaaa aacttcattc ccaatcccgg acgaagaact gctttaccgt
     6001 tttgaaaaac tatataccgg agctgtaaat gatgtattgc gtgaattctg cctgctcgaa
     6061 caagctttgc ccaaacggat catcccttta agagaataca gatctgtggc aggttttgct
     6121 tttacggtaa aaagttcacc aaatgttaag atacagggag agatggaatt tcgggcacaa
     6181 atgcttgatg aaatgcacaa agatgctttt gtaatctggg atacaagccg tgacgaaaaa
     6241 gcaacgttat gggggggagt aatgactgcc actgccaaaa gtaaaaaagt taaggcagcc
     6301 tgtatcgatg gaggtatccg tgatacacat cagattctag aagcagattt cccggtattc
     6361 tacgaatatc gaatctcaaa cggaagcctt gggcgctgtt taattacgca ttaccaagtc
     6421 cctgttaaaa ttggcgatgt aatcgttaaa cccggtgatg tgatattggg agatattgat
     6481 ggtgttttgg ttgttccccg aaatatcgca ttcgatatcc tccttcgtgc agaggaaatc
     6541 aaagagaatg aaaaacgaat ttttgaatgg gtacgcgagg gacaatcggt tcacgacatc
     6601 acagaaaagg gtggatattt ttaattgcag ggtaaccggt tgtaccaatc gtctttagtt
     6661 tagttacaca tttaagcttc cctgggaatt ttctcttgga aactttagta cagtagattt
     6721 gaattttaca aagtataaaa gattttagac ataattctta acaatgaata gaccattatg
     6781 gatttgaaat ctattattta aaataaaaaa gcaatggcaa tgtggtcaaa aatagttggt
     6841 aaaaagcgtt gaaaggcctg cttttattga tagtataga
//
LOCUS       DAOSDR010000152         6878 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrimonas sp. isolate AMR_MDS_1826
            NODE_4014_length_6878_cov_3.987835, whole genome shotgun sequence.
ACCESSION   DAOSDR010000152 DAOSDR010000000
VERSION     DAOSDR010000152.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863355
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrimonas sp. (wastewater metagenome)
  ORGANISM  Petrimonas sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Dysgonomonadaceae; Petrimonas.
REFERENCE   1  (bases 1 to 6878)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6878)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:10:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,720
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 35
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6878
                     /organism="Petrimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4014_length_6878_cov_3.987835"
                     /isolate="AMR_MDS_1826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2023866"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..576
                     /locus_tag="PLI30_07155"
     CDS             <1..576
                     /locus_tag="PLI30_07155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008759583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FumA C-terminus/TtdB family hydratase beta
                     subunit"
                     /protein_id="HOI79368.1"
                     /translation="GGGVKINLNRPMPEILAELSKYPVSTRLSLNGTIIVGRDIAHAK
                     LKERIDRGEGLPQYIKDHPIYYAGPAKTPKGYASGSMGPTTAGRMDSYVDLFQSHGGS
                     MIMLAKGNRSQQVTDACQKHGGFYLGSIGGPAAILAQNSIKSLECVEYPELGMEAIWK
                     IEVEDFPAFILVDDKGNDFFKVVTQPNCALG"
     gene            648..1502
                     /locus_tag="PLI30_07160"
     CDS             648..1502
                     /locus_tag="PLI30_07160"
                     /inference="COORDINATES: protein motif:HMM:NF022188.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2520 domain-containing protein"
                     /protein_id="HOI79369.1"
                     /translation="MKIAFIGAGQVSTALGLYFKNNGFEIGGYYSRSCKSTERAARLT
                     HSHPYASAGELINDNQMIWITTPDDQIEAVVRQITLLPPPRKDEKLVLHASGAYPLSI
                     LNPLKENGYQTACAHPLLAFGDPVVAQEKLGNVWFAIEKPGEDNGQLTGFFKACGNQT
                     FTVDPGKKSLYHAAACVLSNYLVTLLDASFAIFEKSGMPRDNIQEAARPLLESVILNL
                     KGKDLKDALTGPIKRGDKNTVRMHLESLNALMPEMTALYTLMGRKTMQLLGDYSLEEV
                     LNTPLSKQ"
     gene            1508..2341
                     /gene="panB"
                     /locus_tag="PLI30_07165"
     CDS             1508..2341
                     /gene="panB"
                     /locus_tag="PLI30_07165"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009245056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HOI79370.1"
                     /translation="MAQKFTVKSFQEAKDNNRKISMLTAYDYSMAKIVDAAGIDSVLV
                     GDSLGMVFQGNESTLPVTLDEIIYHTKAVVRGVKNALVVADMPFLSYHVSKEEAVRNA
                     GRMIKEGGAEAVKMEGGTLFSETIKAVVDAQIPVMGHIGLTPQSVNAFGGFKVQGKDE
                     QSAKRIWEDAKLLEEAGVFAITLECIPDKLAQLITKSLRIPTIGIGAGKSCDGQVLVI
                     NDMLGMFSDFVPRFIKQYAKLNTEITAAVQDYIAEVREGDFPAGEHTFDMDADILEKL
                     Y"
     gene            2405..3250
                     /gene="panC"
                     /locus_tag="PLI30_07170"
     CDS             2405..3250
                     /gene="panC"
                     /locus_tag="PLI30_07170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004847070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HOI79371.1"
                     /translation="MIITTVKELREYLGAQRTSGKSIGFVPTMGFLHEGHLSLIRRAK
                     AENDTVVVSDFVNPTQFAPNEDFESYPRNIERDEQLATGAGADVVFYPSAEEIYPRGS
                     STFVEVAGDITRVLCGASRPAHFKGVTTVVAILFNIVQPRRAYFGQKDAQQAAILIKM
                     VADLYMDIELIVCPIVREEDGLAMSSRNTYLSATERAQAVILNKALREGQAAFEKGED
                     NVEKLIRQITGKINEAPLACIDYVSIYAYPSLKTIETVSDKALAAVAVRFGKTRLIDN
                     IILEK"
     gene            3251..3607
                     /locus_tag="PLI30_07175"
     CDS             3251..3607
                     /locus_tag="PLI30_07175"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004068 - aspartate 1-decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006523 - alanine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HOI79372.1"
                     /translation="MQLTLLKAKLHRATVTEANLNYTGSITIDKTLLEQSGILVWEKV
                     SVVNNNNGQRFETYVIEGEAGSGTICLNGAAARLVQPGDKIIIMAYVSLSPEEAKEHK
                     PGVFILDEDNRITERI"
     gene            3611..4393
                     /locus_tag="PLI30_07180"
     CDS             3611..4393
                     /locus_tag="PLI30_07180"
                     /inference="COORDINATES: protein motif:HMM:NF016051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenoylcysteine decarboxylase"
                     /protein_id="HOI79373.1"
                     /translation="MLNIAITAGGTSEHIDGVRRLTNISTGLLGWYCLETILDYFCAE
                     KRSDFHVTYLFTETAFRKALDKEQLPFVDFVPVTDAESVYHAVDALTKSVPVDFFIHS
                     MAISDFTLAYVASTEELAKEINSLPYSGDAGATVREVLENPENRYSLTEKIPSDHNII
                     LGLRRTQKVIPLIKRNNPNTFLVGFKLLKDVPEEELIRVANQLAEENGCDMVFANELA
                     QLGESNHLGMLIRSGKVVDRPIGKKQIAEAIVREMMKQGGNK"
     gene            4390..4932
                     /gene="coaBC"
                     /locus_tag="PLI30_07185"
     CDS             4390..4932
                     /gene="coaBC"
                     /locus_tag="PLI30_07185"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015939 - pantothenate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HOI79374.1"
                     /translation="MKTIVIGVSGGIACYKAVEIVNQLKKDGHNVEVIMTRSAQEFVT
                     PLTFRTMSHNPVVTDMFDPVREWDTKHIDLAKAADLFVIVPATANIIGKIASGIADDM
                     LTTTVMAARSPVLIFPAMNTFMYENPIVQDNIEKLKKWGYHVYETAEGELACGDFGKG
                     KLLPWEEIVKIIEEFDLPDD"
     gene            complement(4966..6816)
                     /locus_tag="PLI30_07190"
     CDS             complement(4966..6816)
                     /locus_tag="PLI30_07190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005675668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HOI79375.1"
                     /translation="MVITFIILIATAALFVWGKIRSDIVALCSLLGLLLTGVLTPVEA
                     LSGFSNPIVIMIAALFIVGGAIFQTGLAAKVSKRLLRFAGNSDYKLFLLVISVTALIG
                     SVLSNTGTVALLMPIVVSMATSSNMSVRRLLMPMAFASSIGGMMTLIGTPPILIVHNT
                     LVESGHRGFSFFTTLPVGIILLFCGILLLWPLSKTLDKKRKKDDDDNRSTVKSPKQLV
                     TEYRLAENMYRVETNGKSPVVGKKLSELDITKQYAVTIAEIRRRSTSPFKKTVTQELP
                     EAGTVLGDNDVLYLIGDFVNVARFAEEKDLKFIDQSSDDSMPTFSGKLKFDEIGMAEV
                     VVLANSNIIGHPVRESGFRKNYNVNIIGIQRKTRYLVQNVKDEKIQSGDMLLVQGAWE
                     DIDRLSRLQAEIVVIGQPSAEASKVTLEDKAPVAGIIMGLMVVAMIFNFFPPVVAVLM
                     AAVAMILAGCFRSVRDAYRTINWESVILFAAMMPLATAMEKTGASELISSGIVGSLGS
                     RGPYLVLAGIYLGTSILTMFISNTATAILFAPIALQAAVALGVSPYPFLVAVAVAASM
                     CFASPFSTPPNAMVVSAGRYSFLDYVRVGLPLQVFYMVIMVFALPLVFPF"
BASE COUNT         2004 a         1688 c         1662 g         1524 t
ORIGIN      
        1 ggcggcggcg tgaagatcaa cctcaaccgc ccgatgccgg aaatcctggc ggaactgtcc
       61 aaatacccgg tttcaacgcg gctgtcgctt aacggcacca ttattgtcgg gcgcgacata
      121 gcacacgcca agctcaaaga gcggatcgac cgcggagaag gtcttccaca atacatcaaa
      181 gaccatccca tctactatgc cgggccggca aaaacgccca aaggctatgc ttcgggatcc
      241 atgggaccga ccacggcagg gcggatggat tcctacgtag acctgttcca atcgcacggc
      301 ggcagcatga tcatgctcgc taaagggaac cgcagccagc aggtgaccga tgcttgtcag
      361 aaacacggag ggttctacct gggcagcata gggggtccgg cggccattct ggcacaaaat
      421 agcatcaaga gcctcgaatg cgtggaatat cccgaactgg gtatggaagc catctggaaa
      481 atcgaagtgg aggatttccc cgctttcatt ttggtggacg acaaagggaa cgacttcttc
      541 aaagtagtta cccaacccaa ttgcgcattg ggataacttc ttaaaaacat tttcatttca
      601 tattattttg tacctttgcc tcgcagaaca gcgacgtaaa attggtaatg aagattgctt
      661 ttataggtgc cggccaagtt tcaaccgctt taggattgta tttcaaaaac aacggttttg
      721 agataggagg ttattatagc cgaagttgta aaagcaccga aagggcggcc cggctgaccc
      781 attcccatcc ttatgcatct gcaggggagc tgatcaatga caaccagatg atatggatca
      841 cgacacccga tgaccagatt gaagccgttg tccggcaaat cacccttctc cctccccccc
      901 ggaaggacga aaagctggtc ttgcacgcca gcggcgcata tcctctttcc atactgaacc
      961 cgctaaaaga aaacggctat caaacggctt gcgcacatcc gcttttggca ttcggcgatc
     1021 ccgttgtagc acaggaaaaa ttaggcaacg tatggtttgc catcgagaag ccgggggaag
     1081 ataacggaca attgaccggc tttttcaagg cgtgcggaaa tcaaacgttc accgtagacc
     1141 ccggcaagaa atccctttat cacgcggccg cctgcgtttt gtcgaactac ctggtaacgc
     1201 tgttggatgc ctcctttgcg attttcgaga aatcgggcat gccccgagac aatattcagg
     1261 aagcagcccg gcctttattg gaaagcgtta tccttaacct aaaagggaaa gatcttaaag
     1321 atgcgcttac cggtcccatt aagcgaggcg acaaaaacac cgtccggatg catttggaaa
     1381 gtttgaatgc tcttatgccg gaaatgacag cgttatacac gctgatggga agaaaaacca
     1441 tgcagctgtt gggcgattat tcgctggaag aagtacttaa caccccgctc tctaagcaat
     1501 aaaaaatatg gcacaaaaat ttactgtaaa atcctttcag gaagcgaaag acaataaccg
     1561 gaaaataagt atgctcaccg cctatgatta ttcgatggcg aaaatagtgg atgccgcagg
     1621 aatcgattcc gtcctggttg gcgattcact gggaatggtc ttccagggaa acgaatcaac
     1681 gctcccggtt actcttgacg aaataatata tcacacaaaa gccgttgtcc ggggtgtgaa
     1741 aaatgcgctg gtagtcgccg acatgccttt tttatcctat catgtttcaa aagaagaagc
     1801 tgtgcggaat gcaggcagaa tgatcaagga aggtggtgct gaagccgtca agatggaagg
     1861 cggcacactt ttctcggaaa caataaaagc cgttgtggat gcacaaattc cggtaatggg
     1921 gcatatcggg ttaacaccgc agtcggtcaa cgctttcggc gggtttaaag tacagggaaa
     1981 agacgaacaa agcgcaaaga gaatctggga agatgccaaa ttgctggagg aagctggcgt
     2041 ttttgccatc acactggaat gcattcccga caagctggcc caactgatca caaaatccct
     2101 ccgcatcccg accataggca taggagccgg aaaaagttgt gacggccagg tattggtaat
     2161 caacgacatg ctaggaatgt tcagcgattt tgttccccgg tttataaagc aatacgcaaa
     2221 gctgaacacc gaaattacgg cagctgtcca agattacatt gcagaagtcc gggaaggcga
     2281 ctttccggcc ggagaacaca cgtttgatat ggatgcggat attctggaaa agctgtattg
     2341 aagagcaagg agcaaagaac aaagacaata gacaatagac agtttaacgt tcataattcg
     2401 taaaatgatc attacaaccg taaaagaatt gagggagtat ctcggtgcgc aacgcacatc
     2461 gggaaaatcc ataggctttg tcccgaccat gggattcctg cacgaagggc atctctcgct
     2521 tatccgtagg gcaaaagccg aaaacgatac ggtagtcgtc agcgacttcg ttaatcccac
     2581 ccagtttgcc cccaacgaag atttcgaatc ctatccccgg aacattgaac gcgacgaaca
     2641 actcgccaca ggtgccggag ccgatgtggt gttctatcct tccgcggaag agatctatcc
     2701 ccggggaagt tccacttttg tggaagtcgc cggcgacatc acccgggtac tttgcggagc
     2761 ttcacgcccg gctcatttca aaggtgttac aacggttgtg gccattctct tcaatatcgt
     2821 tcagccgcga agggcctatt tcggacaaaa agatgcacaa caggcggcta ttctgataaa
     2881 aatggtagcc gatttgtaca tggacatcga gctgatcgtc tgtccgattg tcagagaaga
     2941 agacgggctg gccatgagtt cgcgcaacac ctatttatcg gctaccgaaa gggcgcaagc
     3001 tgtcatattg aacaaagcgc tgcgggaagg acaggcggca ttcgaaaaag gggaagacaa
     3061 cgtcgaaaaa ctgatacggc aaattaccgg taagataaac gaagcgccgc ttgcctgcat
     3121 cgattacgta agcatatatg catatccgtc gttaaaaacg atagagaccg tcagcgacaa
     3181 agcgcttgcg gctgttgcag taagattcgg caaaacaaga ttaatcgaca acataatttt
     3241 agaaaaatag atgcaactca ctttattgaa agcaaaactt catagggcga ccgttacgga
     3301 agccaatctc aattatacgg gcagtatcac catcgataaa accttgcttg agcaaagcgg
     3361 aattttggtt tgggaaaagg ttagcgtggt gaacaacaac aacgggcagc ggtttgagac
     3421 ctacgtcatc gaaggagagg caggcagcgg caccatttgc ctgaacggtg cggcggcccg
     3481 gttggtacaa cccggcgaca aaatcatcat catggcatat gtttccctgt cgcccgaaga
     3541 agcaaaagag cacaaacccg gtgtatttat tttggatgag gacaaccgga taaccgaaag
     3601 gatataattt atgctgaata tagccatcac ggcaggcgga acatcggagc atatcgatgg
     3661 agtgcggagg ctgaccaaca tcagcaccgg actgcttggc tggtattgcc tggaaacaat
     3721 cctggactac ttttgcgctg aaaaacgttc cgatttccac gtaacttatc tcttcaccga
     3781 aacggctttc aggaaggcgt tagataaaga gcaacttccg ttcgtggact ttgtccccgt
     3841 caccgatgcg gaaagtgttt accatgccgt ggatgcgtta accaaaagtg ttccggttga
     3901 tttcttcatc cattccatgg ccatatccga tttcacgctt gcctatgtcg caagtacgga
     3961 ggaattggca aaagaaataa acagcttgcc gtacagcggt gacgctggag cgaccgtgag
     4021 agaagtgctc gaaaatccgg aaaacaggta tagcctgacg gaaaaaatcc cgtcggacca
     4081 caacattatt ctgggtctga gacgcaccca aaaagtgatt ccgctaatta aacgaaacaa
     4141 tccgaatacc tttctggtgg gattcaaact gctgaaagat gtgccggaag aagaactgat
     4201 ccgggttgcc aaccaacttg ccgaagaaaa cggatgtgac atggtttttg ccaacgaact
     4261 ggcgcaactg ggggagagca atcatctcgg catgttgata agatcgggga aagtcgttga
     4321 ccggccgatc gggaagaaac aaatagctga agctattgta agggaaatga tgaaacaagg
     4381 aggaaataaa tgaaaacgat cgttatcgga gtttcaggcg gaattgcctg ctacaaagcc
     4441 gtagaaattg tcaatcagct gaaaaaggac gggcacaacg tggaagtgat catgacccga
     4501 tccgcccagg agtttgtcac gccgctcact tttagaacta tgtcgcacaa tccggttgtc
     4561 actgacatgt ttgacccggt acgggaatgg gacaccaagc atatcgacct ggccaaggca
     4621 gccgacctgt tcgtcattgt gcccgccaca gccaacatta tcgggaagat cgcctcggga
     4681 attgcagacg acatgctcac taccaccgtg atggcagccc gaagcccggt acttattttc
     4741 cctgccatga acacctttat gtatgaaaat ccgattgttc aggacaacat cgaaaaactg
     4801 aaaaaatggg gataccacgt ctatgaaacc gctgaaggcg agcttgcctg cggtgatttc
     4861 ggaaaaggga aactactgcc atgggaggaa atcgtgaaga ttatcgaaga gtttgattta
     4921 cccgatgact gatacataat accgcaactc ttatttttcc ggggttcaga acgggaacac
     4981 caaaggcaag gcaaacacca taattaccat gtagaaaacc tgcagcggta atcccaccct
     5041 aacgtaatcc aaaaagctgt accggcctgc cgatacaacc atggcgttgg gcggtgtgga
     5101 aaaaggactt gcaaaacaca tactggcagc aaccgctacc gcaaccagga aaggataagg
     5161 gctgacaccc aacgcaacgg cagcctgcag tgcaatgggc gcaaacaaaa tagccgttgc
     5221 cgtgttgctg ataaacatcg tgagaatgga ggtacccaaa taaattccgg ccaataccaa
     5281 ataagggcca cgacttccca gcgaaccgac tatacccgaa gagataagtt ctgatgctcc
     5341 ggttttctcc atagccgtag ccaaaggcat catggcggca aacaagatga cgctttccca
     5401 gttaatcgta cgataagcat cgcgtacgct tcgaaaacat cccgcaagga tcatggcgac
     5461 agcagccatc aataccgcca ccacgggcgg aaaaaagtta aagatcatcg caacaaccat
     5521 caatcccata ataattcccg caacgggtgc tttgtcttca agcgttacct tggatgcctc
     5581 tgccgagggt tgcccgatga ctacaatttc agcttgcaaa cggcttaacc tgtcaatgtc
     5641 ttcccacgca ccctggacca acaacatatc gcccgactgt attttttcgt ctttcacatt
     5701 ttgcaccaga tagcgcgtct tgcgttgtat gccgataata tttacgttgt agttcttccg
     5761 gaaacccgac tcccttaccg ggtgaccaat gatgttggag ttggcaagca caaccacctc
     5821 ggccatacca atctcatcga acttcagttt tcccgaaaaa gtaggcatgg agtcgtcgga
     5881 ggactgatcg atgaatttaa ggtccttctc ttcagcaaac cgggcaacgt taacaaaatc
     5941 gccaatcagg tacaacacgt cattatcgcc aagtacggtc ccggcttcag gcaattcctg
     6001 ggtaacggtt ttcttgaacg gcgacgtcga cctgcggcgt atttcggcga tagtcacggc
     6061 atattgcttc gttatatcga gttcggaaag ttttttcccg accaccggag atttcccgtt
     6121 cgtttccacc ctgtacatgt tttctgccaa ccggtattcc gttaccagct gcttgggaga
     6181 cttcactgtc gaacggttgt catcgtcgtc tttcttcctt tttttatcaa gtgttttgct
     6241 caaaggccat aataataaaa ttccgcaaaa caacaaaatt attcctacgg gtaatgtggt
     6301 aaaaaacgaa aatccacgat gcccagactc aacaagcgta ttatgtacga taaggattgg
     6361 cggcgttcca ataagtgtca tcataccacc tatgctgctg gcgaaagcca tcggcatcaa
     6421 caaacgccgg acactcatgt tggaggaggt ggccatactc accacgatgg gcataagcag
     6481 cgctaccgtc cccgtattgc tcaacactga tccgataagc gcggttacgg atatgacaag
     6541 taaaaataac ttgtagtcac tgttcccggc aaaacgcaac aaccttttgc ttaccttcgc
     6601 ggccaacccc gtttgaaaaa tagcaccacc cacgataaag agcgccgcaa tcataatgac
     6661 aatggggttg gaaaatccgg acaatgcctc cacaggtgtc agaacacctg tgagcaaaag
     6721 gcccagcaac gaacaaagcg ctacaatatc ggaacgtatt ttcccccaga caaacagagc
     6781 tgctgttgca atcaatataa tgaaagtaat aaccatatca taaaagtgaa caatccaaca
     6841 ttttcaaaaa tgttttttca tcgataacct tgatcccc
//
LOCUS       DAOSDR010000153         6843 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrimonas sp. isolate AMR_MDS_1826
            NODE_4042_length_6843_cov_4.173394, whole genome shotgun sequence.
ACCESSION   DAOSDR010000153 DAOSDR010000000
VERSION     DAOSDR010000153.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863355
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrimonas sp. (wastewater metagenome)
  ORGANISM  Petrimonas sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Dysgonomonadaceae; Petrimonas.
REFERENCE   1  (bases 1 to 6843)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6843)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:10:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,720
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 35
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6843
                     /organism="Petrimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4042_length_6843_cov_4.173394"
                     /isolate="AMR_MDS_1826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2023866"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..370
                     /locus_tag="PLI30_07195"
     CDS             <1..370
                     /locus_tag="PLI30_07195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018337389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="3-keto-5-aminohexanoate cleavage protein"
                     /protein_id="HOI79376.1"
                     /translation="ALHLAAKGYVPGPPMQFNFVLGVPGCAAATVENLVWMVNRIPAG
                     STWTATGIGRSEFILAAHAIAMGGHVRVGFEDNLYLSRSVLAKSNGELVNKVVQMAKL
                     LNREIATPAEARAILGLNPL"
     gene            448..1488
                     /locus_tag="PLI30_07200"
     CDS             448..1488
                     /locus_tag="PLI30_07200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013613319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-erythro-3,5-diaminohexanoate dehydrogenase"
                     /protein_id="HOI79377.1"
                     /translation="MKKGNKYGTHRVIEPKGVLPQPANKIDNNMDEIYDNEILIDVQT
                     LNIDSASFTDISARAGGDHEKIKEIMFEIVATQGKHRNPWTGSGGMLLGTVERIGDAL
                     VGKTDLKIGDKIATLVSLSLTPLRIDNIKEIRDEVDQVDIDGKAILFESGIYARIPAD
                     LPEKLALSALDVAGAPAQTAKLVKPGDTVVIIGAGGKSGMLCSYEAKKRAGITGKVIG
                     ITHSQKSTDRLTTLGFCDHVFPADATQPVAVLEKIEALTSGQLADVTINNVNIPDTEM
                     TSILITKDTGVVYFFSMATSFTKAALGAEGVGSDVTMIVGNGYTRGHAEITLQLLRES
                     EKLRNIFTELYA"
     gene            1576..2814
                     /gene="ablA"
                     /locus_tag="PLI30_07205"
     CDS             1576..2814
                     /gene="ablA"
                     /locus_tag="PLI30_07205"
                     /EC_number="5.4.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018337392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050066 - lysine 2,3-aminomutase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0046440 - L-lysine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine 2,3-aminomutase"
                     /protein_id="HOI79378.1"
                     /translation="MSTSIRQFFSHVPDEQWNDWKWQVKNRIESLEALKQHIHLTREE
                     EEGVRESLKTLRMAITPYYLSLINREDPHCPIRKQAIPTINELHISRGDLDDPLHEDG
                     DSPVPGLTHRYPDRVLFLITDMCAMYCRHCTRRRFAGQKDAGAPIERIDNCINYIART
                     PEIRDVLLSGGDALLMSDDRIEYILRRLRDIPHVEIIRFGTRVPVVMPQRITDELCEM
                     IKKYHPIWLNTHFNHPNEITPEATEACNKLSAAGVPLGNQTVLLRGINDCTYVMRELV
                     HGLGRIRVRPYYIYICDQSKGIGHFRTPISKGIEIIENLRGHTSGYLVPTFVVDAPGG
                     GGKVPVMPTYMISQSPNRVIVRNYEGVISAYTEPEDYKEDCHCPECAKQKKEGVAALL
                     SGRQLSLEPKDLNRFKRNGH"
     gene            2816..3847
                     /locus_tag="PLI30_07210"
     CDS             2816..3847
                     /locus_tag="PLI30_07210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018337393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI79379.1"
                     /translation="MPFIDDILSHRSVSIVGLEKNTGKTETLNYVLNRLKRKNVQVAV
                     TSIGVDGERTDSVTLTPKPEITLFEGMIFVTSEKHYSERRIVSEILEVGDVRTALGRL
                     ITARVKLPGKVVLSGPPDTAGMKKLIDGMAHFGVQTTIVDGALSRLSLASPVVTEAMI
                     LSTGASLSANIPQLVRETRFVQQLIELPCVEGKLVRELQPLKGGMWAIDADGRVHDLE
                     IPSVFLLDKREKDIFRFGTRIYVPGAVSDKLLSILRLQNRNVELMVSDFTKLFVSQKE
                     FNAFLKSGNSMKSVVRSKLIAVTVNPLSSSGFWLDPDSLQREMNKALNVPVYDVKRII
                     HPTDSNQEF"
     gene            3844..4776
                     /locus_tag="PLI30_07215"
     CDS             3844..4776
                     /locus_tag="PLI30_07215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI79380.1"
                     /translation="MTIRQAIQQISGFGYILNSLNILSPAGRKELFSLPFLTNRNDIE
                     TAMDETETAFNIVNTTENERLLSVLFSRLTQLRDISGTVRLLSTKNTLTDIELFEIKH
                     FALLAESVRELAGQLKISFAAIPVLGKIIDILDPEKKRIPHFYVYDRYSPALAALRTQ
                     LSRMSGQECDEQETEPVRLQAQLLEDKIRKDLVQQLFPHAPALSKALHKIARLDVVFA
                     KALQVKESGLCRPTVDDQRTAYTALFHPEIRNLLRGQHKDFQPVDITVPMQPTVITGA
                     NMSGKSVLLKSVALAQTMMQFGFYVAANRQPLCL"
     gene            4767..5228
                     /locus_tag="PLI30_07220"
     CDS             4767..5228
                     /locus_tag="PLI30_07220"
                     /inference="COORDINATES: protein motif:HMM:NF012700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI79381.1"
                     /translation="MPVEQIVISVGDSEDELKGLSSFAAEMLRLNTAIDNTNQQVKQL
                     VLIDEPARTTNPEEGKAIVCGILDFFIQHNVQSLITTHYSIGIPCRKLRVKGFTENRN
                     NEKITVANINSFIDYSLEETAEKEVPHEALKIAEIIGVNETILERIKKYIE"
     gene            5231..6796
                     /locus_tag="PLI30_07225"
     CDS             5231..6796
                     /locus_tag="PLI30_07225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018027936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine 5,6-aminomutase subunit alpha"
                     /protein_id="HOI79382.1"
                     /translation="MVRSKLGIDFKKVKYAREIAGSIAADVHSFADNYTTVAVERTLC
                     RLVGIDDIDDAGVPYPNVLVDDLKRKNVLEQGVLFFLANAVIETELPPQQIAERVASE
                     SLDITAFPFRSKEEIARALQPFVDTSIRQIAERRKKRERYLETIGEGSRPYLYVIVAT
                     GNIYEDVVQAEAAARQGADIIAVIRTTGQSLLDYVPYGATTEGFGGTYATQENFRIMR
                     RALDNVGEEIGRYIRLCNYCSGLCMPEIAAMGALEGLDVMLNDALYGILFRDINMQRT
                     MVDQFMSRVINGFAGVIINTGEDNYLTTADAVEEAHTVLASDLINEQLALLAGLPEEQ
                     MGLGHAFEMDPDLENGFLFELAQAQLTREIFPKAPLKYMPPTKFMTGNIFKGHLQDAL
                     FNMIGVWTSQGIQLLGMPTEAVHTPFMSDRYLSIENARYIFNNMRNLGDEVEFKAGGI
                     IQKRAREVLDNTIALLEELQEGGLFNALEKGIFGNIKRAKNGGKGLDGVSVKGTQYYN
                     PFIDLMNPKPDKL"
BASE COUNT         1916 a         1748 c         1721 g         1458 t
ORIGIN      
        1 cgcacttcac ctagcagcca aaggatatgt ccccggcccg cccatgcagt tcaactttgt
       61 tctcggtgtt cccggatgtg ccgcggcaac cgtcgagaac ctggtatgga tggtgaaccg
      121 gattccggcc ggatcaacgt ggactgccac cggaataggg cggagcgaat ttatccttgc
      181 cgcacacgcc atcgccatgg gaggacacgt acgcgtggga ttcgaagaca acctatacct
      241 gtctcgcagc gtattggcaa aatccaacgg tgaactcgta aataaagtgg ttcaaatggc
      301 aaaactcctg aaccgggaga tcgccacgcc tgcggaagca cgagctattc tgggattaaa
      361 cccattatga gtaaacagga ataaattacc acgaatagaa tcaacaggat tgcttcctgg
      421 cccaaaccta aaaacaaata taaccacatg aaaaaaggaa acaagtacgg cacacaccgc
      481 gtcattgagc cgaaaggcgt gttgccgcaa ccggcaaaca aaatcgacaa caacatggat
      541 gagatttacg acaacgaaat cctcatcgac gtacagaccc tcaacatcga ctcggcatcg
      601 tttaccgata tctcggcgcg tgccggaggc gaccacgaga agataaagga aatcatgttc
      661 gaaatcgttg ccacacaggg taaacaccgc aatccgtgga cgggttcggg agggatgttg
      721 ctgggaacgg tcgaaagaat cggggatgcg ctcgtcggaa agaccgacct aaaaattgga
      781 gacaagattg ccaccctggt ttccctctcg cttaccccgc tgcgtattga caacatcaag
      841 gagatccgcg acgaagtgga ccaggtagat atcgacggga aagccatcct cttcgagagt
      901 gggatctacg cccggatacc tgcagacttg cccgagaagc tggccctctc ggcgttagac
      961 gttgcagggg cgcctgcaca aaccgccaaa ttggtgaaac cgggagacac ggtggtgatt
     1021 atcggcgccg gcggaaaatc gggaatgctc tgcagctacg aggccaaaaa aagggccggc
     1081 attactggaa aagttatagg gataacccat tcgcaaaaga gtaccgaccg gcttaccaca
     1141 ctcggctttt gcgatcacgt ctttccggcc gatgccacgc aaccggtggc cgttctggaa
     1201 aaaatagaag cgctcaccag cgggcaactt gccgacgtta ccatcaacaa cgtaaatatc
     1261 cccgataccg aaatgaccag tatccttatc accaaagata cgggtgtggt ttatttcttc
     1321 tctatggcaa ccagtttcac gaaagcggct ctcggagccg aaggtgtggg cagtgacgtg
     1381 accatgatcg tgggcaacgg ttacacccgc ggacacgccg aaatcacgct tcaacttctc
     1441 cgggagtcgg aaaaactgcg gaatatcttt acggaattat acgcctaatt agcagaacta
     1501 tgagcaaagg gtgaaggata ctccgctctt tactctttgc ccaacgctca aagcataacg
     1561 caaatcataa ataacatgag cacttcaatc cgccaatttt tttctcacgt tcccgacgaa
     1621 caatggaacg attggaaatg gcaagtgaag aaccgcattg aatcacttga agccctgaaa
     1681 caacatatcc acctgacacg ggaagaagag gaaggggtac gcgaatcgct aaaaacactg
     1741 cggatggcca tcacgcctta ttacctgagt ttgataaaca gggaagatcc gcattgcccc
     1801 atccgcaagc aggcgattcc tactattaat gagctgcaca tctcccgtgg cgacctcgac
     1861 gaccccctgc atgaagacgg cgactctccc gtgcccggac tcacgcatcg gtatcccgac
     1921 cgtgtacttt ttctcatcac cgatatgtgt gccatgtact gccgccattg cacccgccgc
     1981 cgatttgccg ggcaaaagga tgccggcgcg cccatcgaac gtatcgataa ttgcatcaac
     2041 tacattgcca ggaccccgga aatccgcgac gtactccttt ctggcggtga cgcgctattg
     2101 atgagcgacg acaggataga atatatcctc aggcgcttgc gcgacattcc gcatgtcgaa
     2161 atcattcgtt tcggaacccg cgtgcctgtg gtcatgccgc aacgtatcac ggatgagctc
     2221 tgcgagatga taaaaaaata ccatcccata tggctcaaca cacatttcaa ccaccccaac
     2281 gaaatcacgc ctgaagccac cgaagcctgc aacaagcttt ctgctgccgg ggtacccctc
     2341 ggcaaccaaa ccgtactgct gcgcggcatc aacgactgta cttacgtcat gcgtgaactc
     2401 gtacacggcc tggggcgcat tcgtgtacgg ccatattaca tctatatttg cgaccagtca
     2461 aaaggaatcg gccacttccg cacgccgatt tcaaaaggca ttgaaatcat tgaaaacctg
     2521 cgggggcata cttccggtta cctcgtaccc acctttgtgg tggatgcacc cggcggagga
     2581 gggaaggtcc ccgtgatgcc cacatacatg atttcgcaat cgcccaaccg tgtcattgtg
     2641 cgcaactacg aaggtgttat cagtgcgtac accgagccgg aagactacaa ggaagattgc
     2701 cattgccccg aatgcgccaa acagaaaaaa gaaggtgtgg cagcgttact ttcgggacgg
     2761 caattatcgc tggaacccaa agacttgaac aggtttaaac ggaacggaca ctgaagtgcc
     2821 ctttatcgac gacatattat cccaccgcag cgtttcgatc gtaggactcg aaaaaaacac
     2881 gggaaaaacc gaaacgttga actacgtcct aaatcgcctg aagaggaaga acgtgcaagt
     2941 ggcagtgacc tcgatcggtg tagacggaga acgcaccgac agcgttacgc taacacccaa
     3001 gccggaaata acactgttcg agggaatgat ttttgtgacc tccgagaaac actattccga
     3061 acggagaatt gtatcggaga ttttggaggt tggcgacgta cgaacggcat taggcagatt
     3121 gataacggca cgtgtcaaat taccgggaaa agttgtcctt tccggaccac cagacaccgc
     3181 aggaatgaag aagcttatcg atggaatggc ccattttggc gttcaaacca caatcgtgga
     3241 cggtgcactg tcgcgcctga gtcttgcctc acctgttgtt accgaggcaa tgattctttc
     3301 cacgggagcg tcactttcgg caaacatccc gcagttggtg cgcgaaacgc gctttgtgca
     3361 gcagctaatc gaattgccgt gcgttgaggg gaaacttgtt cgggaactgc aaccgttaaa
     3421 aggcggtatg tgggcgatag acgccgatgg ccgtgtgcac gatttggaaa taccctcggt
     3481 attccttctc gataaaaggg aaaaggatat tttccggttt ggtacgcgaa tatacgtacc
     3541 gggagccgta agcgataagc tgctttcaat actgcggtta cagaacagga atgttgaact
     3601 gatggtgagc gatttcacaa aattatttgt ctcgcaaaaa gaattcaacg cattcctgaa
     3661 aagtggaaat tcgatgaaga gtgtcgtacg aagcaagctc attgccgtga cggtaaatcc
     3721 attatcgtcc agcggctttt ggctcgatcc cgactcgctt caacgggaaa tgaacaaagc
     3781 actgaatgtg ccggtatatg atgtgaaaag aataattcat ccaacggaca gcaaccaaga
     3841 attttgacca taagacaagc catacagcaa atcagcggat tcggttatat cctaaactcg
     3901 ctgaacattc tttctcccgc cggaagaaag gagctgtttt ctttgccgtt cctgacaaac
     3961 cgaaacgata ttgaaacagc tatggatgaa actgaaacgg ctttcaacat cgtcaacaca
     4021 acggagaacg agagactact gtcggtgctc ttttcgaggc tgacgcagtt acgcgacatt
     4081 tcagggaccg taagactcct ctcaaccaaa aacacattga ccgatattga actgttcgaa
     4141 ataaaacatt tcgccctgct tgcagaatcc gtgcgcgaac ttgccggaca attgaaaatt
     4201 tcttttgcag ctatccctgt tctcggaaaa attatcgata tcctcgaccc ggaaaaaaaa
     4261 cgaattcctc acttttacgt gtacgaccgt tattcaccgg ccctggcagc gctgcgtact
     4321 caattaagca ggatgtcggg gcaagagtgt gacgaacagg aaacagagcc ggttcgttta
     4381 caggcgcaac tgctagagga taagatccga aaagaccttg tacaacagct ttttccgcac
     4441 gctccagcct tgtcgaaagc cttgcacaaa attgcccggc ttgatgttgt gtttgccaaa
     4501 gcccttcagg taaaagaatc aggattatgc aggccaaccg tcgacgacca gagaaccgca
     4561 tacactgcac tgttccatcc tgaaatccgg aacttgttgc gggggcaaca caaggatttt
     4621 cagccggtgg atatcactgt cccgatgcaa cccaccgtga ttaccggagc caacatgtcg
     4681 ggcaagagcg ttctgctgaa aagcgtggcc ctggcacaaa caatgatgca gttcgggttt
     4741 tacgttgcgg ccaatcggca accgttgtgc ctgtagaaca aattgttatc agcgtgggtg
     4801 acagcgagga cgaacttaag ggactctctt cttttgctgc cgaaatgctt cgcctgaaca
     4861 ccgctatcga caacacgaac caacaggtta agcaacttgt acttattgat gaaccggccc
     4921 gtaccaccaa tccggaagaa ggcaaggcca tagtgtgcgg gatactcgac ttcttcattc
     4981 aacacaacgt gcaatcgctt atcaccacac actacagcat cggtataccc tgccggaaac
     5041 tgcgcgtgaa aggatttaca gaaaacagaa acaacgaaaa aataaccgtc gccaacatca
     5101 actctttcat cgactattcg ctggaagaaa ccgcggaaaa ggaagtgccg cacgaagcat
     5161 tgaaaattgc ggagataatt ggtgttaatg aaactatttt agaacggata aaaaaatata
     5221 ttgaataggt atggtcagaa gtaaactggg gatagatttc aagaaagtaa aatacgcacg
     5281 cgaaattgcc ggcagtattg ctgccgacgt gcattctttt gccgacaatt acaccacggt
     5341 ggccgttgag cggacactct gccgtttagt gggaatagac gatatcgacg atgccggggt
     5401 accgtatccg aacgtgctgg tagacgatct gaaaagaaag aatgtattgg agcagggtgt
     5461 tcttttcttt ttggccaacg cggttatcga aacggagctg ccgccgcagc aaattgctga
     5521 acgggtggct tcggagagct tggatatcac ggcttttccg tttcgctcta aagaagagat
     5581 cgcccgtgca ttgcaaccgt ttgtcgatac aagcatccgt caaatagcgg agagacggaa
     5641 aaaaagggaa cgttatctcg agaccatcgg cgaaggaagc cgtccgtacc tttatgtgat
     5701 agtggcaacc ggcaacatct acgaagatgt ggtgcaagcc gaagccgccg caaggcaggg
     5761 ggcagacatt atcgccgtta ttcgcaccac ggggcaaagc ctgctcgatt atgtgcctta
     5821 tggtgctacc accgaagggt tcggcggaac gtacgccacg caggaaaatt tccgcatcat
     5881 gcgccgtgcg cttgacaacg tgggtgaaga gatcggaaga tacatccgtt tgtgcaacta
     5941 ttgttccgga ttgtgtatgc ccgaaattgc cgcgatggga gccctggaag gactggacgt
     6001 aatgcttaac gacgcgcttt acggcatcct tttccgcgac atcaacatgc agcgtacgat
     6061 ggtcgaccag tttatgtcgc gtgtcatcaa cgggtttgca ggtgtcatca tcaacaccgg
     6121 agaagacaac tacctcacca ctgccgatgc cgtggaagag gcgcataccg tgctggcatc
     6181 agacctgatt aatgaacagc tggccctgct tgccggcctt cccgaagagc aaatggggct
     6241 ggggcacgct tttgaaatgg atcccgatct cgagaatggg ttccttttcg aactcgcaca
     6301 agcacagttg acccgcgaaa tattccccaa ggcgccactg aaatacatgc cgcccacaaa
     6361 attcatgacg gggaacattt tcaaggggca cctgcaggac gcgcttttca atatgatcgg
     6421 cgtttggaca tcgcaaggaa ttcagctgct ggggatgccc accgaagccg ttcacacacc
     6481 cttcatgagc gaccgctacc tatccataga aaatgcccga tacatcttca acaatatgcg
     6541 taaccttggc gatgaagtgg agtttaaagc gggcggaatc atccaaaaaa gagccaggga
     6601 ggtgttggac aataccatcg cattgctcga ggaacttcag gagggagggc tcttcaatgc
     6661 actggaaaaa ggtatttttg gcaatataaa acgtgccaag aacggtggaa aaggattgga
     6721 cggcgtatcg gtgaaaggaa cgcaatatta caatccgttt attgacttaa tgaaccccaa
     6781 acccgataaa ttatgagcga aggactgtac aagacccgcc cctgcgattt cgaccagacg
     6841 ttg
//
LOCUS       DAOSDR010000154         6809 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrimonas sp. isolate AMR_MDS_1826
            NODE_4074_length_6809_cov_3.734380, whole genome shotgun sequence.
ACCESSION   DAOSDR010000154 DAOSDR010000000
VERSION     DAOSDR010000154.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863355
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrimonas sp. (wastewater metagenome)
  ORGANISM  Petrimonas sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Dysgonomonadaceae; Petrimonas.
REFERENCE   1  (bases 1 to 6809)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6809)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:10:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,720
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 35
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6809
                     /organism="Petrimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4074_length_6809_cov_3.734380"
                     /isolate="AMR_MDS_1826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2023866"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2774)
                     /locus_tag="PLI30_07230"
     CDS             complement(<1..2774)
                     /locus_tag="PLI30_07230"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HOI79383.1"
                     /translation="MNITEISIKRPSLIIVLFGIFALMGTIGYINLSYELLPDFNQPV
                     VVIKTMYPGAESQEVETSVSRKIEDALSNLEGVDYLETKSMPNASVIIANLKYGINVD
                     KAMQDAQRYIDNIIKDLPDDVQSPVMSKVSPNDLPIISISATSNLPSTSFYQKMKDDF
                     LPQIQQLKGVAEITVLGGEEREIQVQVDQEKLKLYKVSLPQVVDAVNRSGIDLPAGKV
                     QSEKENNAVRLTGKFASVEDIMNVQVAMPVPGSPVYVKDVASIVDGVKEISSISRYNG
                     KNGIGLLLKKQGDANAVDVSELVREKLKSIEEKNKADDIKFIIADDSTDNTIAAVNSV
                     VHDLILAVILVSLVMLLFLRSYRNSVIVLISIPTSLITAFAVMWLLGYTLNLMTLLAM
                     SLIIGILVDDSIVILENIQRHLDMGKEKRTASLEGRMEIGFSALSITLVDVVVFLPIL
                     FLQVFVADMLKQFSVVVVTSTLTSLLVGFTLTPWLTSRIGKKDDLKPTNAFNRFLLWF
                     EQQLETFIQWYGQQLEWVLSHKAAFTAIILLLFLITFGIMKHGIIGKEMMSTGDQGKF
                     RLNLEYDKITTVQQNNIVSQQVENFILQHPEVSSLFSNIAGPSTGIGSLGVGSANKSE
                     FTIQLKPESERGIKTEAFMKNLRNELEAEFPGINFSMAVLGLLPKSAPIKITLSGEDL
                     DLVLKTSEKLKEAVKQIPGADNVQLSVEEGNPEYKVVPDKDKMQKLGLTTAYTGLNLR
                     TAFTGNDEASLTEQGTEYPVRIWLDNFNRRNFEDVQRLAVVNPMNIPVEVSQFAEVFP
                     DNSPSMLERLDRQASVTLTAESFGRPSGSLAGEVITYLEAHPLPDNIKLTWGADIKTQ
                     NESFGALGSVLLISFILIYLIMVALYDSFIYPFVALFGIPVAAIGAFLALNLTANDIT
                     LFALLGLIML"
     gene            complement(2784..3839)
                     /locus_tag="PLI30_07235"
     CDS             complement(2784..3839)
                     /locus_tag="PLI30_07235"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HOI79384.1"
                     /translation="MWKKIIYTTIILILIVFTAIRLKKNKEISQERVYRYEKEKPISV
                     QTIILKKEPLGNDYSYSGTFEPNKETKISSDIQGKVNAVLVESGSVVHKGQTLIRLDN
                     SLLKLQLQNIEVQIEGLEADVKRYTVLAKADAIQGIQLEKAELGLKSARIQRATLMEQ
                     IDKTEIRSPFNGIVTAKLTEEGAFAAPGVPLMQITDISTLKFTLNVPENKVIQFNDSK
                     EGQVTTDVYPNFTLPAKVSMVGSKANTGNSYPVQFVVSNTPDLKIKSGMFGKVRIKTD
                     TEEGEQIIIPASAVVGTNIQPQVYIVKSGQAILQDVIISKRTGNKLIISNGLNEGDEI
                     VTNGFINLFDGARVNIK"
     gene            complement(3847..5190)
                     /locus_tag="PLI30_07240"
     CDS             complement(3847..5190)
                     /locus_tag="PLI30_07240"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HOI79385.1"
                     /translation="MKRLTKKTIKEEYLKIFFIGILSVASNISPAQTWSLQQCIDTAL
                     VHNKQLTMSKNNISLAQQKQQESKANLIPKINITADYKYFVDLPYQLMPQSAFGGPEG
                     VFKEMQFGVPHNIGANIQITMPLYNPQVYGAIQSTKIISELNQLQYKKTEEQLFFEIS
                     NLYYNVQILQHQLKFTEGNLANTGKLLKNMELLHEQLMVKQSDVDKVQLQKEQLTTSH
                     KLIVANLEQVMNSLKFTMGISIYQNIAIDSMIQYQENREYHVLPIVDLQIANTQSRLL
                     TSELSTLKNSKFPTLSLYGSYGQNGFGYDEKPNDFLKFFPVSFAGLQISYPLFNGTIT
                     KRKIGQKKIEIENSRLQTDLVTEQNHMMTDNAKHRKTITQETIKNTLSQIDLATKVFE
                     QTLLQQKEGTAGLTEVLLADSALREAQQNYLSAIVDYLRADLELKKLTGNISLTK"
     gene            complement(5216..5767)
                     /locus_tag="PLI30_07245"
     CDS             complement(5216..5767)
                     /locus_tag="PLI30_07245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI79386.1"
                     /translation="MDSITETIIEAAGKAIFSYGIRNFTIESLAGRPEIKGTGLPPHI
                     QQKEDVFVLLLIKFEYELHKMVDSIRGQALSPALEIESLFRNLSGMFREKPYYLAIIF
                     DSELKEQNKKTKEIIARIKGRAENYLTALIDRGKKEKAFLTAENTKTLTNHILNSFRF
                     LMNDLLLYAELSKNLKMLKPYKN"
     gene            complement(6290..>6809)
                     /locus_tag="PLI30_07250"
     CDS             complement(6290..>6809)
                     /locus_tag="PLI30_07250"
                     /inference="COORDINATES: protein motif:HMM:NF019958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HOI79387.1"
                     /translation="GIQGLTIKNLSKKLGITEPAIYRHFDNKIHILLTVLDLLKYNSE
                     KIFESEWNMEKNAVRKIERVFEKHFKSFSDMPPLAAVIFSEEMFRNEKKLVDKVSEII
                     EHNNLVLLTILKEGQQNNEIRNDLDTSHLAIIIMGSLRLFVKKWLFSGFSFDLQAEGG
                     KLITSVKILIAK"
BASE COUNT         1751 a         1360 c         1423 g         2275 t
ORIGIN      
        1 atgagcataa tcagtccgag cagtgcaaac aaggttatgt cgtttgccgt caggttaagg
       61 gccagaaatg caccgatagc ggctaccggg attccgaaca aggccacaaa cggatagata
      121 aaactatcgt aaagcgcaac cattatcaga tagatcagga tgaaagagat cagtaaaaca
      181 gaacctaaag ccccgaaact ttcattttgt gttttaatat ctgcacccca ggttaatttt
      241 atgttgtcgg gtaacggatg ggcttccaga taggtgatta cctcaccagc cagtgaaccg
      301 gaagggcgtc caaacgattc ggccgtcaat gttaccgatg cctgccgatc gaggcgttcg
      361 agcatcgacg gcgaattatc cggaaaaact tcagcaaact gggacacttc aaccggtata
      421 ttcatcggat tcacaactgc caggcgctgt acatcttcaa agtttctccg gttgaaatta
      481 tccagccata ttcttaccgg atattcggtg ccttgttcag ttagcgaggc ttcgtcattt
      541 ccggtaaaag cggtcctcag gttcaatccg gtatacgcag tggtcaatcc cagcttttgc
      601 atcttgtctt tatcaggtac caccttatat tccggatttc cttcttcaac cgatagttga
      661 acgttatcgg cgccagggat ttgtttgaca gcctctttca gtttttctga ggttttcaac
      721 acaaggtcca aatcttcacc gctcagcgtg atttttatag gcgccgattt gggaagtaat
      781 ccaaggacag ccatcgaaaa gttaataccc gggaactcgg cttccaactc attgcgtaag
      841 tttttcataa aagcttcggt tttaatgccc ctttccgatt ccggtttcaa ctgtatggtg
      901 aattcagatt tgtttgcaga acctactccc aaacttccaa ttcccgtgct tggaccggca
      961 atattgctga aaagggaaga aacttcaggg tgttggagga tgaaattttc aacctgttgc
     1021 gaaacgatat tgttctgctg gacggtcgtg attttgtcgt actccaggtt tagtctgaac
     1081 tttccctggt ctccggttga catcatctct ttcccgatga taccatgctt cataattcca
     1141 aaggtgatta aaaaaagcag cagaataatg gcagtaaaag cggccttgtg gcttagcacc
     1201 cattccagct gttgtccata ccattgaata aaagtctcaa gctgttgttc aaaccaaagc
     1261 aggaagcggt taaaggcgtt ggtgggcttc agatcatctt tttttccgat gcgtgaagtc
     1321 agccagggag tcagtgtaaa acccaccagc aaactggtga gagttgaagt cacgacgact
     1381 acggaaaact gcttaagcat atcggccaca aaaacctgta aaaacaaaat gggcaggaaa
     1441 accaccacat cgaccaaggt gatggaaagc gccgagaacc caatttccat gcggccttcc
     1501 aatgatgcgg ttcgtttttc ttttcccata tccaggtggc gctgtatgtt ttccagtatt
     1561 acaatagagt cgtcaaccag tatcccgata attaaagaca tggcgagcaa ggtcatcagg
     1621 ttcagggtat agccaagaag ccacattaca gcaaaagcag tgatcagaga tgtagggatt
     1681 gaaatcagca caataaccga attccgatag cttcttaaaa ataaaagcat gacaagcgaa
     1741 accaggatta ctgccaatat caaatcgtgg acaaccgagt ttactgctgc aatagtattg
     1801 tcggtggagt catctgcaat aataaatttt atatcgtcgg ctttgttttt ttcttcgatg
     1861 gatttcagct tttcacgtac caactccgaa acatcgactg cgtttgcatc gccttgtttt
     1921 ttcagcaaaa gtccaattcc gtttttccca ttgtaacgac ttatggagga gatttctttt
     1981 accccatcga caatactggc gacgtctttt acataaacgg ggcttccggg gacaggcatt
     2041 gccacctgca cattcataat atcttccaca gaggcaaatt tcccggttag gcgcacggca
     2101 ttgttttctt tttcagactg gactttaccg gccggcaaat cgattccgga gcggttaact
     2161 gcgtctacaa cctggggaag ggagacttta tataacttca gtttttcctg atctacctgt
     2221 acctggattt cgcgttcttc gcccccgagc acggtaatct cggcaacacc tttcagctgc
     2281 tggatttgtg gcagaaaatc atctttcatt ttctggtaaa agctggttga cggtagatta
     2341 ctggttgcgc tgatggatat aatgggcaaa tcgttgggtg atactttgct cattaccggg
     2401 ctttgtacat cgtcgggcag gtcttttata atgttgtcga tgtatcgctg agcatcctgc
     2461 atggctttgt cgacattaat gccgtatttc aggttggcaa tgattactga agcattcggc
     2521 attgatttgg tctccaggta atcgactcct tcgaggttcg acaaagcatc ttcaattttt
     2581 ctcgaaacag atgtttcaac ctcctgagat tcggcgcccg gatacatggt tttgatcact
     2641 actaccggct ggttaaaatc gggtaacaac tcgtagctca ggttgatata gcctattgta
     2701 cccattagtg cgaaaatacc aaagagtacg attatcagcg atggccgttt gatggagatt
     2761 tctgtaatat tcatgtttgc aaattatttg atgtttaccc ttgccccgtc aaaaaggttg
     2821 ataaaaccgt tggtgacaat ttcgtctcct tcgtttaatc catttgatat gatgagctta
     2881 ttgccggttc tttttgaaat gataacgtct tgtagaatag cttgtccgct ttttacaata
     2941 tagacctgcg gctgaatatt ggtgccaaca actgcagaag cagggataat aatctgttcc
     3001 ccttcttctg tatcggtttt gattctcacc tttccgaaca ttcccgattt gatttttaaa
     3061 tcgggtgtat tgcttacaac aaactgaacc ggatagctat ttcccgtatt tgccttgctt
     3121 ccgaccatgc tcacttttgc cggcaatgta aagttggggt aaacatcggt agtcacctga
     3181 ccttctttgc tgtcattaaa ttgaatcacc ttgttctctg gaacattgag tgtaaatttc
     3241 agagtcgaaa tatctgtaat ttgcatcaag gggactccgg gagcggcaaa ggcgccttct
     3301 tcggttagtt ttgcggtaac aatgccatta aaaggcgatc tgatttctgt tttatcaatc
     3361 tgttccataa gagtagccct ttggatccgg gctgacttta agcccaactc tgctttttcc
     3421 agttgaattc cctggatggc atccgctttt gccagcacag tatatcgttt tacgtcagcc
     3481 tccaatcctt caatctgtac ttcaatgttt tgcagttgaa gtttcaaaag ggaattgtcg
     3541 agccggatta atgtttgtcc tttgtgtact acgcttccgg attccaccaa tactgcatta
     3601 actttgccct ggatatctga actgatcttt gtttccttgt tgggctcgaa tgtgcctgaa
     3661 taggaataat catttccgag aggctctttc tttaaaatga tggtttgaac gctaatcggt
     3721 ttttcttttt catagcggta caccctttcc tgactgattt ccttgttttt tttaaggcgt
     3781 attgccgtaa acactatcag tatcagtatg atagttgtat aaatgatttt tttccacatg
     3841 gtctttctat tttgttagtg aaatatttcc ggttagtttt ttaagttcca gatcggccct
     3901 gagatagtca actattgctg acaagtagtt ttgctgggct tcacggagtg cactgtcggc
     3961 caacaatacc tcagttaatc cggcagttcc ttctttctgc tgtaacaagg tctgttcaaa
     4021 tacttttgtt gccaggtcaa tttgagacaa ggtatttttg atagtttcct gtgtgattgt
     4081 tttgcggtgt ttggcgttgt cggtcatcat gtggttttgc tccgtgacca ggtctgtctg
     4141 tagcctgctg ttttcaatct ctatcttttt ctgaccgatt tttcttttcg taatagtacc
     4201 gttaaacaaa ggatatgaaa tctggagccc tgcaaaactt acaggaaaaa atttaagaaa
     4261 gtcattgggt ttttcatcat acccgaatcc gttttgccca tagcttccat aaagagacaa
     4321 tgtcggaaac ttggagtttt tcagtgtaga taactcgctt gttaaaagcc ggctttgtgt
     4381 attggctatt tgtaagtcaa ctatcggtag gacatggtac tccctgtttt cctgatattg
     4441 tatcatcgag tcaattgcga tgttttgata gatagagatt cccattgtga atttcaggga
     4501 attcataacc tgttccaaat tggctacaat aagtttgtga ctggttgtta actgttcttt
     4561 ttgtaactgc actttatcca catcgctttg ttttaccatt aactgttcat ggagcaattc
     4621 catatttttg agtagcttac ctgtgtttgc caggttgcct tctgtaaatt ttaactggtg
     4681 ttgcaatatc tgaacattat aatacaggtt ggaaatttca aaaaagagtt gttcctctgt
     4741 ttttttatat tgaagttgat ttagctctga gataattttg gtgctttgaa tcgcaccata
     4801 aacctgcgga ttgtacaatg gcatggtaat ttgaatgttt gcccctatgt tatgaggaac
     4861 cccaaactgc atttccttaa agactccttc gggccctcca aaggccgact ggggcatcag
     4921 ttgataaggc aggtcgacaa aatatttata atctgccgtt atgttgattt tgggtatcag
     4981 gttggccttt gactcctgct gtttttgctg agccagtgaa atattatttt tgctcattgt
     5041 aagttgcttg ttgtgtacca gggcagtgtc tatgcactgc tggagcgacc aggtctgagc
     5101 cggtgaaata ttactcgcaa cggataatat tcctataaaa aagattttga gatactcttc
     5161 tttaattgtt ttttttgtaa gccttttcat attctgttgt cagattatca gtttcttaat
     5221 ttttgtatgg ctttaacatt tttaagtttt ttgatagctc tgcatacagg agtaagtcgt
     5281 tcatcaaaaa acgaaaactg ttcaggatgt gattcgtcag ggttttagtg ttttctgcgg
     5341 tcagaaaggc tttttctttt ttcccccggt cgataagggc cgttaaatag ttttcagctc
     5401 tgccttttat ccgggcgatg atctcttttg tttttttatt ctgttctttt aattcactgt
     5461 caaatattat tgccaggtaa tagggcttct ccctgaacat gccggacagg ttcctgaaaa
     5521 gagattcaat ttcaagggca ggagacaacg cttggcctct gatactgtcg accattttgt
     5581 gtaattcgta ttcaaatttg attagtagca aaacaaaaac atcttccttt tgctgtatgt
     5641 ggggaggtaa gccagtccct tttatttcag gacggccggc aagagattcg atggtgaaat
     5701 ttcttatccc gtaggaaaat attgctttcc cggctgcttc aataattgtt tctgttatgg
     5761 aatccatgtc tttaattgtt tttaaatcaa aacaaagtta gttaatattt actaacatgc
     5821 aatatttctt gatttttttg tcaatatttc agttttttca gggtgaactg ccatcttttt
     5881 ttcatcagga tttttgtttt gtgtaggtta tttgttgata ttcaaagtga taagattagc
     5941 ttgcaggaaa gaaggagtgt ggttctataa aatcgtttac cgcaaagtgt atgaattctt
     6001 tctgtccgtt ggctgtaagg cagaatacct ttataaatat cgtttggaat aggttgttgc
     6061 acttatgcaa gcataattcc ttagagttgg gatgttttaa cgaaagatgt tccaggtatg
     6121 gaaatgggct tgtgtggcaa atgccatttt atctgttatc tattcatgaa gtatagcaaa
     6181 ttaaagtata acgactttaa tgcatagaga taactgaaat atcacagatt tttagatttg
     6241 atattggggg ttgagataag tatgcgcttt aaatctatga gttttgctgt tattttgcga
     6301 ttaaaatttt aaccgatgta attaattttc ccccttcagc ctgtaaatca aaagaaaagc
     6361 cggagaagag ccattttttt acaaataacc gtaaagaacc cataattata atagccaggt
     6421 ggctggtgtc caaatcgttt ctgatttcat tgttctgttg cccttcttta agaatggtta
     6481 gcaataccaa attgttgtgt tcaatgatct ctgaaacttt gtccacaagt tttttttcat
     6541 ttcgaaacat ttcttctgaa aaaatcacag cagccagcgg aggcatatca gaaaacgact
     6601 tgaaatgttt ttcaaatacc cgttcaattt ttctgacagc atttttttcc atattccatt
     6661 ctgattcgaa tattttttcg ctgttgtatt tcagcagatc gaggacagtc agtaatatgt
     6721 ggattttatt gtcaaaatgt ctgtaaatgg caggttcggt gatacccagt ttctttgaaa
     6781 ggttttttat agttaatcct tgaatgcct
//
LOCUS       DAOSDR010000155         6773 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrimonas sp. isolate AMR_MDS_1826
            NODE_4097_length_6773_cov_4.026645, whole genome shotgun sequence.
ACCESSION   DAOSDR010000155 DAOSDR010000000
VERSION     DAOSDR010000155.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863355
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrimonas sp. (wastewater metagenome)
  ORGANISM  Petrimonas sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Dysgonomonadaceae; Petrimonas.
REFERENCE   1  (bases 1 to 6773)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6773)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:10:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,720
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 35
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6773
                     /organism="Petrimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4097_length_6773_cov_4.026645"
                     /isolate="AMR_MDS_1826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2023866"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(22..1803)
                     /locus_tag="PLI30_07255"
     CDS             complement(22..1803)
                     /locus_tag="PLI30_07255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010265055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6067 family protein"
                     /protein_id="HOI79388.1"
                     /translation="MRIAKTTYRYALGVFLGLPVLTAVACNNNRPEKECITFEEMKDP
                     TNDTLSDWSHVTAGLHSSFISVDDKLPKSVAPEITLVNNVKISGWKGEKVSAQLLLWT
                     AEDIDQVEYAFNDFKTEGNSLPASVAQARFVRYVMTDEFGPGCGHRQPGDFPASLAPD
                     MLDNLECFNLDAKTVRPLWITFDIPSDATAGNYKGELTIYARGQSPHNFEIELEIVDR
                     LLPEPSEWSYHLDLWQHPSAVARVHNLELWSDAHFEKMKPLMQMLADAGQKVITATLN
                     KDPWNNQCFDAYADMIVWTKNKDRSWSFDYTVFDRWVQFMMDLGINKMINCYSLLTWN
                     NQLHYNDMEKGELVTVELKAQSKEYEELWGVFLKDFTRHLREKGWLEKTNIAMDERGP
                     ADMKAALRVLELYAPELGVSLADNHKSYREYPWIKDICVGASNKVSREEIEARRAKGL
                     TTTYYVCCADKFPNMFTFSPSAESVYAAWYAAALDLDGFLRWAYNSWVENPLTDSRFR
                     TWPAGDTYFVYPDARSSIRFERLVEGIQDAEKIRVLRKKYTGENTPESLKKLAQLEEA
                     IAGFGTLEPSSDWQKRLSDAKRLLNTL"
     gene            complement(1834..5055)
                     /locus_tag="PLI30_07260"
     CDS             complement(1834..5055)
                     /locus_tag="PLI30_07260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018666594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HOI79389.1"
                     /translation="MSLIVCGRPTAGMLSAQEQSDRYAEIINPKLLHINREEPRSTFS
                     SFTDLKEALAAGYISKGSDVVLLNGSWKFHYTDRFSQRPKEGFQKADFDDSGWNDIRV
                     PGNWEVQGFGVPIYVNTTYEFTSPGHTPYWDRPNPPLVPEEFNPTGTYRKTFTLPDNW
                     QEKEIILVADAARGAAYYYLNGEFVGMNKEGKLPARFNVTDQAKAGENVLAVQIHRWS
                     SGSYLECQDFWRLSGFDRDVYLYARPKLHLADIFAHPGLDASYTNGQFALDITVASPE
                     GNTRPFTLTYTLSDEAGKKVAGGSLTEKVSERASVRFEEMIPGVKKWSAETPHLYTLA
                     VELMDEKGSTLEATALKVGFRTAEIKNRQFLVNGQPILLKGVNLHEHNEHTGHYVTEE
                     LIRKDFELFRKYNVNAVRTSHYPQPELFYRLADEYGIYVIDEANIESHGMGYNLRKGG
                     TLGNNPLFLEDHLSRTSGMVERDKNHPCVITWSLGNEAGNGYNFYETYRWIKSRDTSR
                     PVQYERAVMEWNSDIFAPMYSDPDEIERYANDPASDRPLILCEYAHAMGNSLGNFTEY
                     WDIIRKYPLLQGGFIWDWVDQGLVKNDEKGNRFWAYGGDYGPTGTPSSGDFCINGVVF
                     PDRSVKPHTEEMRKVYQHVWFRNFNPVDGSVELFNEHFFSDLSNYQITYSIKTEGKEL
                     AQGRLQAEAAPQEAVKVAVPGWARYAGRKEQLTVNFTVTQQKEERLIPAGWVVARNQF
                     VVNDYSAQITGKLKAAGTEETETSVIVSGRRFKAVFDKQTGLLTSYRVDGTEYIENGA
                     GPRPFFWRAPIDNDYGARLPKRLSAWREASYRELRAEGLKITRGETTTLTYRYSYPET
                     GASNEVTYTLYDNGTLHISNRFDATNSDTEMIPRIGIRMQLTGELVQADYYGRGPWEN
                     YADRKSSAFLDRYTSPINEMVTRYVLPQENGHHTDTRWLALTRKSGNGLLFVADDLFE
                     FNVSNYLLETITNGESLNNDAPAGTAPRNKHISDYRPSDVVDLFIDHRMQGVGGNNSW
                     GKLPLEEYLIRPGEASATYGFTLIPIRNRKEVERHILSSSTEN"
     gene            complement(5185..6525)
                     /locus_tag="PLI30_07265"
     CDS             complement(5185..6525)
                     /locus_tag="PLI30_07265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019539299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOI79390.1"
                     /translation="MQNRREFLKKMAIATVALNSGTSVLHAAENTLAKNVKMSVSPNS
                     YINLGLIGKGGMGTSDTLTALTVKGVKLVGVCDLYDKRLKDAKAQWGNEIFVTKDYRE
                     ILLRKDIDAVIIATPDHWHQPIAIEAMKAGKHIYCEKPVIHKLAEGKGLIEAQKKSGV
                     LFQVGSQGMASLGNRAARLLIRQGIIGAVNFVDGQFTSAPGMLNAFTAPDDASEETIW
                     WSRFLGNAPKIPFNPHHFFEWRNWKDYGTGIAGDLFVHVLSSIHYIMDAIGPEKVYTT
                     GGIHHYTDGSRDTPDIMLGYFDYPDRNNTGAFTVQLGANYVDGVSKKWGSMDFRIVGS
                     KGSLDVGWDKIVLKTTGDVNTKDFEALGELGQEFDAPRKVSSREYVFNAKKGYKGGHY
                     DHHFNFFNGIRNNTPLTADVLFAVRTAAPALLSYESYLRNEAIKWDAQKLKIRK"
BASE COUNT         1386 a         1744 c         1768 g         1875 t
ORIGIN      
        1 ttttcatttt tacgtcacgt tctaaagcgt gttcagcaac ctcttggcgt cgctcaatct
       61 cttctgccag tcgcttgaag gttccagcgt gccgaacccg gcaatcgcct cttctagctg
      121 cgccagcttc ttcagggatt cgggcgtgtt ctctccggtg tactttttcc gcagtacccg
      181 gattttttcc gcgtcctgaa tcccttccac cagccgttca aaccggatgg agcttcgggc
      241 atcgggatac acgaaatagg tgtctcctgc cggccaggtc cggaaacggg agtcggtcag
      301 cgggttctct acccaggagt tataggccca gcgcaggaat ccgtctaaat cgagggcagc
      361 cgcataccag gctgcgtata ccgattctgc cgagggggag aaagtaaaca tgttgggaaa
      421 tttatcggca caacagacgt agtaagtggt ggtaagccct tttgctcttc gtgcctcgat
      481 ctcttcacgg cttaccttgt tgctggcacc cacgcaaata tcttttatcc acggatattc
      541 gcggtaactc ttgtgattat ccgcaagcga aacacccagc tcgggtgcat agagttccaa
      601 cactctaagg gctgccttca tatcggcagg cccacgttcg tccatcgcga tattggtctt
      661 ttccagccat cccttttccc ggaggtgacg ggtaaagtct ttcaggaaca ctccccacag
      721 ctcttcatac tctttcgact gcgccttcag ctctacggta accagctcgc ctttctccat
      781 atcgttgtag tgcagctggt tattccaggt aaggagagag tagcagttga tcatcttatt
      841 gatgcccaga tccatcatga actgtaccca acggtcgaaa acggtatagt cgaaactcca
      901 gctccggtct ttgttcttcg tccagacaat catgtcggca tacgcatcaa agcactggtt
      961 gttccagggg tccttgttca gggtagcagt aatcactttt tgtcccgcat cggccagcat
     1021 ctgcatcaac ggtttcattt tctcgaaatg ggcgtcgctc cacagctcca ggttgtgtac
     1081 acgggctacg gctgacgggt gctgccacag gtcgaggtgg tagctccatt ctgacggctc
     1141 gggcaacagc cggtcaacga tttccagttc gatctcgaaa ttatggggtg actggccccg
     1201 ggcgtagatg gtaagttctc ccttgtagtt tccggccgta gcatcggaag ggatgtcaaa
     1261 ggtgatccat agcggcctga cggtttttgc atccaggtta aagcattcca ggttatccag
     1321 catgtccggc gccagcgaag cagggaagtc gcccggttgg cggtgcccgc agcccgggcc
     1381 gaactcatcg gtcatgacat aacggacgaa ccgggcttgt gctacagatg cgggcaggct
     1441 gttcccttca gtcttgaagt cgttaaacgc gtactctacc tggtcgatat cctcggcagt
     1501 ccagagcagc agttgggcgg acactttctc gcctttccat ccggaaattt tcacgttgtt
     1561 gacgagggtg atctccggtg ccacggattt gggcagtttg tcatcaacag agatgaacga
     1621 ggagtgcaat cccgcagtta cgtgcgacca gtcggacagg gtgtcgttgg tggggtcttt
     1681 catctcttcg aaggtgatgc actctttttc cggacggttg ttgttacacg ccacagctgt
     1741 aaggaccggt aaccctagaa aaacaccgag ggcataccgg taagttgttt tcgcgattct
     1801 catatctctt ttgtagttta atgccaaaga tagttaattt tcggttgaag aacttaaaat
     1861 atggcgttca acctctttcc tgttcctgat ggggatcagc gtaaaaccgt aggtggccga
     1921 agcttcaccc gggcggatca ggtactcttc cagcggcagc ttgccccagc tgttgttccc
     1981 tcccacaccc tgcatgcggt gatcgataaa gaggtccacc acatccgacg gacggtagtc
     2041 gcttatatgc ttgttccggg gcgcagttcc tgccggtgcg tcgttgttga gcgattctcc
     2101 gttggtgatc gtctccagca ggtagttgga cacgttgaac tcaaacaggt cgtctgctac
     2161 aaagagcagt ccattacccg atttccgggt tagtgccagc cagcgggtat cggtatgatg
     2221 tccgttttcc tgcggcaaca cgtagcgggt taccatttcg ttgatgggtg atgtgtaacg
     2281 gtcgaggaag gcggaacttt ttcggtcggc gtagttctcc caggggcccc tgccgtaata
     2341 atccgcttgc accagctctc ctgtgagctg catccgtata ccgatacggg gtatcatttc
     2401 tgtatctgag ttggtggcat cgaaccggtt ggagatatgg agggtgccgt tgtcatagag
     2461 agtataagtc acttcgttgc tggccccggt ttcggggtag ctgtagcggt aggtgagggt
     2521 tgttgtctcg ccgcgggtga tcttcaggcc ttccgccctg agttcccggt agctggcctc
     2581 tctccatgcg gagagccttt taggaagccg tgcgccgtaa tcgttatcga tgggagcccg
     2641 ccagaagaag ggacggggac cagcaccgtt ttcgatatat tcggtgccgt ccacccggta
     2701 ggaggtgagc agcccggtct gtttgtcgaa gacagccttg aaacggcggc cgcttacgat
     2761 caccgaggtt tctgtctctt ctgttccggc tgctttcagc ttcccggtta tctgggcaga
     2821 gtaatcgttg accacaaact ggttacgggc taccacccag ccggcaggga tcagccgttc
     2881 ctccttctgc tgtgtgaccg taaagttgac ggtcagctgc tctttgcgtc cggcgtaacg
     2941 ggcccagccg ggtaccgcta cctttacggc ctcctgtgga gctgcttctg cctgcagcct
     3001 cccctgggcc agttcctttc cttcggtttt gatgctgtat gtaatctggt aattactcag
     3061 atcggagaag aagtgctcgt tgaagagttc taccgacccg tccaccgggt tgaaattgcg
     3121 gaaccagaca tgttggtaca ccttgcgcat ctcttcggta tgtggtttca cgctccggtc
     3181 ggggaagacc acaccgttga tgcagaagtc acccgaactg ggagtacctg ttggcccgta
     3241 gtcgcctccg taggcccaga aacggttccc tttctcatcg ttcttcacca gtccctggtc
     3301 tacccagtcc cagataaagc ctccctgcaa cagggggtac ttccggatga tgtcccagta
     3361 ttcggtgaag ttgcccaggc tgtttcccat ggcatgagcg tactcacaga ggataagggg
     3421 gcggtcggat gcgggatcgt tggcgtaccg ctcgatctca tcgggatcgg agtacatcgg
     3481 tgcgaagata tcgctgttcc actccattac agcccgttcg tactgcacgg ggcgggaggt
     3541 gtcgcggctt ttgatccagc gataggtctc gtagaaattg tatccgttac cagcctcgtt
     3601 gcccagtgac caggtgatga cgcaggggtg gttcttatcc cgttccacca tgccggaggt
     3661 gcggctcagg tgatcttcca gaaagagcgg gttgtttccc agcgtacctc cttttcggag
     3721 gttgtatccc atcccatggc tttcgatgtt ggcctcgtcg atcacgtaga tcccgtattc
     3781 gtcagccagc ctgtagaaga gttccggttg cgggtagtgg ctggtacgca cggcattgac
     3841 gttgtatttg cggaacagtt cgaaatcttt gcgtatcaac tcttcggtga cgtaatggcc
     3901 ggtatgttcg ttgtgttcat gcaggttcac ccctttcaac agtatcggct gcccgttcac
     3961 taggaactgg cggtttttaa tttctgctgt acggaaacct actttcaggg ctgtcgcttc
     4021 gagggtgctt cccttttcat ccatcagctc aacggctagc gtatagaggt ggggtgtctc
     4081 ggcgctccac tttttcacgc cgggtatcat ctcttcaaag cgaacggatg ctctttcgga
     4141 aactttttca gtgagggatc cgccggccac ttttttgccg gcttcgtccg acagggtgta
     4201 ggtgagagtg aatgggcggg tgttcccttc gggggaagct acggtgatat cgagcgcaaa
     4261 ctgtccgttg gtgtagctgg catcgaggcc cggatgagcg aagatatccg ccaggtgtag
     4321 cttgggacgg gcatagaggt agacatcgcg gtcaaacccc gagagccgcc agaagtcctg
     4381 acactccagg taggaaccac tgctccagcg gtgaatctgt accgccagca cgttttctcc
     4441 cgcttttgcc tgatcggtca cgttgaatcg tgccggtagc ttgccctcct tgttcatgcc
     4501 gacgaactcc ccgttgaggt agtagtaggc tgctcctctg gctgcatctg caacgaggat
     4561 gatctccttt tcctgccagt tgtcgggaag ggtaaatgtt ttccgatagg ttcccgtggg
     4621 gttgaattct tccggaacga gtgggggatt gggcctatcc cagtagggtg tgtggcccgg
     4681 agaggtgaat tcgtaggtgg tattcacata aatgggaaca ccaaatccct gcacctccca
     4741 gttgcccggc acccggatat cgttccatcc gctgtcgtca aaatcagcct tctgaaagcc
     4801 ttctttaggg cgttgactga accggtcggt gtaatgaaac ttccaactcc cgttcagtag
     4861 caccacatcg cttcctttgg atatgtaacc ggcagcaagg gcttccttta gatctgtgaa
     4921 tgaagagaat gttgaccgcg gctcttcgcg gttgatgtgc aacagtttag ggttgatgat
     4981 ctccgcataa cggtctgact gctcctgggc agatagcatg ccggcggtcg gtctaccgca
     5041 tacgatcagg gacatcagga tcagaggaat tgttcttttc atgttatgtt tctttatgtt
     5101 tgatagtgca aaaatattaa ttaaatcttc acaagtgtta caaaaacact taaatcttgc
     5161 ggctaataat tttttgcagc ttatctattt tcttattttg agtttctgag catcccattt
     5221 gattgcttcg ttgcgtaaat agctttcgta actcagcagt gcaggtgctg cagttctgac
     5281 tgcgaagagg acgtctgccg tcaacggtgt attgttcctg attccattga aaaaattgaa
     5341 atgatggtca taatgtcctc ctttgtaccc tttcttcgca ttgaaaacat actcacggga
     5401 ggatactttc cggggagcat caaactcctg tcccaattcc cccaatgctt caaaatcttt
     5461 ggtattcaca tccccggttg ttttcagtac aattttatcc cagcccacgt ccaatgaacc
     5521 ttttgagccg acaatcctga aatccatgct tccccacttt ttggatacgc catccacata
     5581 attggctccc agctgcacag taaaagctcc tgtgttattc ctgtcgggat aatcgaaata
     5641 tcccagcatg atatcgggcg tatcgcgtga accgtcggta tagtgatgta ttcctccggt
     5701 ggtatatacc ttttccgggc ctattgcatc catgatatag tgaatgcttg agaggacatg
     5761 tacaaacagg tctcctgcta tgcccgtacc ataatctttc cagtttctcc attcaaaaaa
     5821 atggtggggg ttgaatggga ttttaggcgc gtttcccaga aaacggctcc accagattgt
     5881 ttcttcagag gcatcatcgg gtgcggtaaa ggcatttagc atgcccggag ctgaagtgaa
     5941 ctgcccgtca acaaaattga ccgctccgat aattccctgc cgaatcagca accgggctgc
     6001 cctgtttccc agggaggcca tgccctgact gcctacctgg aaaaggactc cgcttttttt
     6061 ctgcgcttcg atcaggcctt ttccttctgc cagcttatgg atgactggtt tttcgcaata
     6121 gatgtgtttc ccggccttca tcgcttcaat ggcaatcggc tgatgccaat ggtcgggagt
     6181 agcaataatt accgcgtcga tatctttccg cagaagaatt tcccggtaat ctttcgtaac
     6241 gaaaatttca tttccccatt gcgccttggc atcttttaac cgtttatcgt acaagtcaca
     6301 aaccccaaca agcttaacgc ctttgactgt taatgcggta agggtgtctg aggttcccat
     6361 gcctcccttg ccgatcaggc cgagattgat ataagagttg ggtgataccg acatttttac
     6421 gttctttgcc aatgtatttt ctgctgcgtg tagcacggaa gtgcctgaat tcaaggcgac
     6481 agtggctatg gccatttttt ttagaaactc gcgtctgttt tgcatatgat gtaatttagg
     6541 tataagttgt tttgccggta tatttttctg cacgatttac acttcaaatt tacaaaaact
     6601 tgtgatataa aaacgcgatc gatcagtgtt tgtgctactt tttttagatc ttccgttatc
     6661 tcttcattac ggatggggat aggtgcttgg ttgtattctg cttttttaaa aggagataat
     6721 cttgtccatc agtgcggaga ttttctttaa atgcgttaaa tctccaccgg gaa
//
LOCUS       DAOSDR010000156         6772 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrimonas sp. isolate AMR_MDS_1826
            NODE_4098_length_6772_cov_3.735001, whole genome shotgun sequence.
ACCESSION   DAOSDR010000156 DAOSDR010000000
VERSION     DAOSDR010000156.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863355
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrimonas sp. (wastewater metagenome)
  ORGANISM  Petrimonas sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Dysgonomonadaceae; Petrimonas.
REFERENCE   1  (bases 1 to 6772)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6772)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:10:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,720
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 35
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6772
                     /organism="Petrimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4098_length_6772_cov_3.735001"
                     /isolate="AMR_MDS_1826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2023866"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..303)
                     /locus_tag="PLI30_07270"
     CDS             complement(<1..303)
                     /locus_tag="PLI30_07270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019539710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine beta-lyase"
                     /protein_id="HOI79391.1"
                     /translation="MQYNFDEIIDRSTSHSVKLNKLKTVFGRDDLIPLWVADMDFLSP
                     PAITEALIERTRHGIFGYTVPYDGYFLSIINWLKDRHSYEVYRNEITFVPGVVKGFA"
     gene            complement(305..1150)
                     /locus_tag="PLI30_07275"
     CDS             complement(305..1150)
                     /locus_tag="PLI30_07275"
                     /inference="COORDINATES: protein motif:HMM:NF023205.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3316 domain-containing protein"
                     /protein_id="HOI79392.1"
                     /translation="MKRLPAEITITVTLFLTICSISLSAQESTTKSTNQATLLGVGKS
                     FLTDTYLSPLEYRGLTMSLLHDRISPTKHFNEKLLLQQQFQIQLAFTKNPSASASEYA
                     GEVSYNVNAFYPFFKNTKFSLLAGGGGDASLGGIYNVRNSNNPGSLKASVNLDLSAMA
                     SYNWRRLTFRWQVSTPFLGMFFSPAYGQSYYEIFSLGNGKGTVRFASFQNQLALRNYF
                     TVDIPLNNLTIRTGYLGDFYRTDVNDIVTNIVSHQFMVGLAVESLNFGGLKAKNSKLI
                     KSSYY"
     gene            complement(1273..3999)
                     /locus_tag="PLI30_07280"
     CDS             complement(1273..3999)
                     /locus_tag="PLI30_07280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007656014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 78 glycoside hydrolase catalytic domain"
                     /protein_id="HOI79393.1"
                     /translation="MRHTLLFTVILLSVLLAGCKEGKLNVASLKIEMQENPEGLSTLT
                     PRFSWQNSSNSPDLIQQSYQIQVARSEDDLKKEVNLLWDSGIVSSDGSVLIPYQGQPL
                     SSGSKYFWRVKINTNQGDGNWSKVNYWSMALLNDSEWQAKWIGEDSLSNPGETDKDNT
                     RLAARYLRKSFETRNSTIHRAVLYVAGLGAHEAYLNGNRVSEDVFAPTVSWYPNRVYY
                     NTYDVTPLLQKDKNLLAVKLGNGRYFGMRGSRSQMFGLPRLLAQLDIEYADGTREVIA
                     SDESWKVTSKGPIVANNEFDGEEYDARLELTGWETAGYDDASWQQVDIMTAPEGKLTA
                     QPNPNIRVMEEIKPVRISKLADGKFILDMGQNMVGWLKINKLNGKKDQPVTMRFAEIL
                     NPDSTIYLANIRSAKVTDIYTPAKDGAFSWEPSFVYHGFRFVEISGLNEQPELSSFTG
                     KVVYDNMETTGQFETSNNIINQTFKNAYWGIRGNYRGMPTDCPQRDERQGWLGDRATG
                     CFGEAFVFNNALLYSKWAQDIEDSQSPEGSISVVSPRYWTIYNDDVTWPAAYFYAAKM
                     LYHQYGDTEPIRKHYPSMKRYLERIQQVSMQDFILTKDTYGDWCMPPEKQELIHSQDP
                     SRKTAGAILSTTMYYSLLNLMVEFAQLTGNQQDIQGFKELAGRIKEAYNTTFFNTDSA
                     KYDNNTVTANMLSLRLGLVPEGKEQQVFENIVQKTEVDFNGHVSTGVLGIQQLMRGLT
                     QYGNVDLAYKIATNETYPSWGYMIKKGATTIWELWNGDTADPAMNSANHVMLLGDLII
                     WYYEDLAGIKNYPGSVAYKKLWMEPRFPEGLSHVKASYKSVYGEIKSEWTKENGSFNW
                     NISIPGNTSAVVKLPKELQIIKPGQNGVRSFKETDSGVEIELGSGNYTLSNR"
     gene            complement(4026..>6772)
                     /locus_tag="PLI30_07285"
     CDS             complement(4026..>6772)
                     /locus_tag="PLI30_07285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019537708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="family 78 glycoside hydrolase catalytic domain"
                     /protein_id="HOI79394.1"
                     /translation="ASFSIFSQKQGALYPMELTTEYLVDPTGLDVRQPRFSWKLATAN
                     PQVFGQAQTAYRILVSSTKRDLDKNKGNIWDTGWISSDNMQLVDYKGKELESDRSYFW
                     KVAVKDEKGNPSAFSKSASFTTGLFDDAEWTAKWIGTTEIYDPSAGPNKISDPWFRKS
                     FDLKQKPSRATLFVASVGYHEVYVNGQKIDDHVLAPAVTDHTQRARYIAYDIAPQLKQ
                     GKNVIALWLGASWAIYAPYATPDKPRTPIVTAQTDIFGKNDQKLVRIITDESWKTHPS
                     PNKLIGNWGFGVGGYGGEIWDANKEIDNWNLATYNDQVWNRATVYTPALKLSAQRVET
                     NTLFNEIQPVEIVSRPDGTFRVDMGVNFAGWTQINVDGNPGDTVRFQFSERQQDDMTF
                     GLYNLYVIGPSGKGTFKNRFNYSSGRWITLKGLKSAPKKEDIKGWMVRTNFADATSFE
                     CSDPLQNWIYNTVNWTFENLSLGGYVVDCPQRERFGYGGDAHATSETGMLNYQLGAFY
                     NKWLEDWRDVQGTEPMVGNMNDPDWARKKEGSGRRLGGGILPQTAPTYHGGGGPAWGG
                     IVVTLPWFMYQYQGDVRVLEENFEMIKGWLAFLDTHVENNLLKRYGGRWDFLGDWLWP
                     GATTQGMNNYSDENLFFNNCYWIYNLKTGAKIAEAIGKTAEAQKWKHQAEVSGKALHD
                     KYFHADDHNYSDKTMRSLSAALYGDIMPVKLRPLVMKRLEKEILVNRNGHIDVGITGG
                     AMLFKVLREEGRDDLIYSMTSQTTYPSWGLMRESGASTMWEMWEKDLPGHSLLHSSYL
                     YPGAWYIDGVAGIRRRDDSPGFRKFDIRVPKFAEEHVSWAKAGFNSPAGMIQSHWKRE
                     NGKTTLSVTIPPNCAATVYFPDESGREIRESSGFATETGKKNGYVLFEINSGNYIFEN
                     "
BASE COUNT         1554 a         1632 c         1627 g         1959 t
ORIGIN      
        1 ggcaaagcct ttcactacac ccggaacgaa agtgatttca ttgcggtaaa cttcataact
       61 gtgtctgtct ttgagccagt tgatgatgga aaggaaatac ccgtcgtaag gaaccgtata
      121 tccgaaaata ccgtgtcggg ttctctcgat cagcgcttcg gtaattgccg gaggcgatag
      181 aaaatccatg tcggccaccc agagggggat gaggtcatcg cgcccgaaaa ccgtcttcag
      241 cttattcagc tttacactgt gtgaggttga ccggtcgatg atttcgtcga aattatattg
      301 catatcaata atagctgctt tttattagtt tactgttttt tgccttcaac ccgccaaaat
      361 taagcgactc gacggctaac ccgaccataa actggtgcga aacaatgttt gtaacaatat
      421 cgtttacatc ggttctgtaa aaatcaccca ggtatccggt acggatagtc aggttgttga
      481 ggggaatatc aacggtgaaa tagttacgta acgccaactg gttctggaac gaggcaaacc
      541 ttaccgttcc ttttccgttg cccagggaga aaatctcgta gtaggattgt ccgtatgcgg
      601 gagagaagaa catgcccaga aacggtgtgc ttacctgcca gcgaaaagtg agccttcgcc
      661 agttatagct tgccattgcc gagaggtcaa ggttcacgga agctttcagt gaacccgggt
      721 tgttggagtt tcgtacatta taaatacctc ccagtgatgc atctcctccg ccaccagcga
      781 gaaggctgaa cttcgtgttt ttgaaaaagg gatagaaagc gttgacgttg taggaaactt
      841 cgccggcata ttccgatgcg gatgcagaag gatttttggt aaatgccagt tgtatttgaa
      901 actgctgctg caggagtaac ttttcgttga agtgcttggt gggactgatg cggtcgtgca
      961 acaaagacat agtcaaccct ctgtactcta aaggagataa ataggtgtca gtcagaaaag
     1021 atttgcccac tcccagaagc gtagcttgat tggtggattt tgtcgtactc tcctgagcag
     1081 aaagcgaaat tgaacaaatc gttaaaaata aagtgactgt tattgtaatt tcagcgggta
     1141 accttttcat tcttacttat aattcgatat tcaacttaca aaagtaagaa aatatttaaa
     1201 taaaagccgt tgcccctgtc taaaagtcag tttatttcct ttattaacga tttgttcaat
     1261 tcaaactacc ggttaccggt tggacagggt atagttgcct gatcctaatt ctatttcaac
     1321 tcccgagtcg gtttccttaa agcttcttac gccattttga cccggtttta tgatttgtag
     1381 ctctttcggc aatttcacca cagcggaagt gtttcccgga atagagatgt tccagttaaa
     1441 cgacccgttt tcttttgtcc attcgctctt gatttctccg taaaccgatt tgtacgaagc
     1501 tttcacatgt gaaagtcctt caggaaaccg gggttccatc cacaactttt tgtaagctac
     1561 actcccggga taatttttaa ttccggccaa atcttcgtaa taccagatga tgagatctcc
     1621 gagcaacatg acgtgattgg cggagttcat tgccgggtcg gctgtatccc cgttccaaag
     1681 ttcccaaatg gtggtagcac cttttttgat catgtacccc cagctcggat aggtctcatt
     1741 tgtcgcaatt ttatatgcca ggtccacgtt tccgtactgt gtaagtccac gcatcaactg
     1801 ctgtatcccc agcacaccgg tactgacgtg tccgttaaaa tcgacttctg ttttttgaac
     1861 gatattttca aacacttgct gttcctttcc ttcgggaacc aggcccagcc ggagtgacag
     1921 catgtttgcc gttaccgtgt tgttgtcgta ttttgccgag tcagtgttga agaacgtcgt
     1981 gttgtatgcc tcttttatcc ggcctgccag ctccttaaat ccctgaatat cctgttggtt
     2041 cccggtcaac tgggcaaact caaccatcag gttaagcagg ctgtaataca tggttgtact
     2101 cagaatcgct cccgccgtct tgcgcgaagg atcctgggag tggatcagct cctgtttttc
     2161 gggaggcatg caccaatcgc cgtaggtgtc ttttgtgagg ataaagtctt gcatggatac
     2221 ctgctggata cgctccagat aacgtttcat ggagggataa tgttttctga tgggttccgt
     2281 atcaccgtat tggtggtaaa gcatttttgc tgcatagaaa taagcagccg gccaggttac
     2341 gtcgtcgttg taaatggtcc agtaacgggg tgaaaccacc gaaatacttc cttccggact
     2401 ttgagaatcc tctatgtcct gtgcccattt actgtacagc aaggcgttat taaaaacaaa
     2461 cgcttcgccg aagcatccgg ttgcccggtc gcccagccag ccctggcgtt catcgcgttg
     2521 cggacagtcg gtgggcattc cgcggtagtt tccgcgaatt ccccaatatg cgtttttgaa
     2581 ggtctggttg atgatgttgt tggaagtttc gaactgtccg gtggtctcca tgttgtcgta
     2641 caccactttc ccggtaaaac tggataattc gggttgttca ttcaatccgg atatttcaac
     2701 gaaacggaag ccgtgataaa caaaggaagg ttcccagcta aacgctccgt ctttcgcagg
     2761 ggtgtagatg tccgttacct ttgcggagcg gatatttgct aggtaaatgg tgctgtccgg
     2821 attgagtatc tcagcaaaac gcatcgtcac gggttgatcc tttttcccgt tcaacttgtt
     2881 tatcttcagc cacccgacca tgttttggcc catatcgagg ataaatttac catcggcaag
     2941 cttggagatc cggacgggtt ttatctcttc catcacccgg atattcgggt tgggttgggc
     3001 cgtgagtttg ccttcgggag cagtcattat gtccacttgt tgccacgagg catcatcgta
     3061 gccggctgtt tcccatccgg tcagttcgag gcgggcatcg tattcttctc cgtcaaactc
     3121 attgttggcc acgatgggcc cttttgaagt aactttccag gattcatcac tggcaatcac
     3181 ctccctggtc ccgtcggcat attctatatc gagttgagcc agcaaacggg gcaacccgaa
     3241 catctggctg cgtgatcccc gcattccgaa atacctgccg ttgcccagtt taacggccag
     3301 caggtttttg tctttctgca ggagcggggt tacgtcgtac gtattgtaat aaacccggtt
     3361 gggataccag gaaaccgttg gggcaaagac atcttctgaa acccgattcc cgttcaggta
     3421 agcttcatgc gctccgaggc ctgcaacata aaggacggcc cgatggattg tactgtttct
     3481 cgtctcaaag gactttctca ggtaacgtgc ggccaggcgt gtgttgtcct tatccgtctc
     3541 accgggattg gacaatgagt cctcaccaat ccattttgcc tgccactccg aatcattcaa
     3601 tagagccatc gaccaataat tgactttgct ccagttgccg tcgccttgat tggtgttgat
     3661 ttttacgcgc cagaagtatt tgcttcccga cgagaggggt tggccctggt aaggaatgag
     3721 taccgacccg tcgctgctga cgattcccga atcccacaaa agatttactt ctttttttag
     3781 atcatcttcc gaccgggcaa cttgaatctg atacgattgt tggataagat cgggagaatt
     3841 ggaactattc tgccacgaaa accgtggagt aagtgtgctt aatccttccg gattttcctg
     3901 catttcaatt ttcagggaag ctacattcag ctttccttct ttacatccgg ccaacaggac
     3961 ggaaagtaaa attacggtga ataaaagggt gtgtctcata agaaaaatta gttctagata
     4021 tgttattagt tctcaaagat atagtttccc gaattaattt caaacagaac gtatccgttt
     4081 tttttgcccg tttcggttgc aaaaccggaa ctttcacgta tttccctacc ggattcatcg
     4141 gggaaataaa cggttgctgc gcaattgggt ggaatggtaa cggagagcgt cgttttaccg
     4201 ttttctcttt tccaatgtga ttgaatcata ccggcaggag agttgaagcc tgcttttgcc
     4261 catgaaacgt gctcttctgc aaattttggg acgcggatat cgaactttct gaatccaggc
     4321 gaatcgtctc ttctgcggat tccggctaca ccgtctatgt accatgcgcc gggatagaga
     4381 taggagctgt gtaaaagtga atggccgggt aagtctttct cccacatttc ccacatggtg
     4441 gaagctccgc tttcacgcat cagcccccag ctgggatagg tggtttgtga tgtcatggag
     4501 tagatgaggt cgtcgcggcc ttcttcgcgc aataccttga aaagcatcgc accgccggta
     4561 attcccacat cgatgtggcc gttgcggttg acgaggattt ctttttcgag ccgtttcatt
     4621 accaacggac ggagtttcac cggcattatg tctccgtata acgctgcaga gaggcttcgc
     4681 atggttttat cggaataatt gtggtcatcg gcgtgaaagt atttgtcgtg aagggctttg
     4741 cccgatacct cagcctggtg tttccatttt tgcgcttcgg ccgtttttcc gattgcttcg
     4801 gctatctttg cacctgtctt taagttgtaa atccagtaac agttgttgaa aaacaggttt
     4861 tcgtcggaat agttgttcat cccttgtgtg gttgcaccgg gccaaagcca gtcgcccaaa
     4921 aaatcccacc ggccgccgta tcgtttgagt agattatttt ccacatgggt gtccagaaaa
     4981 gcgagccagc cttttatcat ctcgaaattt tcctcaagta cccgcacatc accctggtat
     5041 tggtacatga accagggtag ggtaaccaca attccgcccc aagcagggcc gccgccgccg
     5101 tgataggtgg gtgccgtctg gggcaatata cccccgccga gtcttctgcc gctgccttcc
     5161 ttcttgcgcg cccagtctgg atcgttcatg ttcccgacca tcggttcggt cccttgtaca
     5221 tcccgccaat cttcgagcca tttgttgtag aacgcgccca gctgataatt cagcattccg
     5281 gtttcggatg tggcgtgggc gtcaccaccg tagccgaacc gttcacgttg cgggcagtca
     5341 acaacgtacc cgccgagaga caggttctcg aatgtccagt ttacggtatt gtaaatccag
     5401 ttttgcaacg gatcggaaca ttcaaacgag gtagcatccg cgaagttggt acgaaccatc
     5461 caacctttta tgtcttcctt cttcggggct gatttcaggc ctttaagggt gatccatcta
     5521 ccggaactgt aattaaaccg gtttttgaat gtgcctttcc cggaagggcc gatgacgtat
     5581 aagttgtata acccaaaggt catatcgtcc tgctggcgtt cagaaaattg gaaacgaacc
     5641 gtgtcgccgg ggtttccgtc aacgttgatc tgcgtccatc ccgcaaaatt tacccccatg
     5701 tctacccgga atgtcccatc ggggcggctt acgatctcta cgggttgaat ttcgttgaac
     5761 agggtatttg tttccacgcg ttgagcggac agttttagtg ccggtgtata aaccgttgcc
     5821 ctgttccata cctgatcatt ataggtcgcc aggttccagt tgtcgatttc tttgttggca
     5881 tcccaaattt caccaccgta tccgccaacg ccaaaccccc agttcccgat gagtttattg
     5941 ggactgggat gtgttttcca cgactcgtcg gtgatgatcc tcaccagttt ttggtcgttc
     6001 tttccgaaaa tatcggtttg ggctgtcaca atgggtgtcc ggggtttatc gggagtggca
     6061 tagggtgcgt agattgccca ggatgctccc agccacaacg cgatgacatt tttcccttgt
     6121 tttaactgcg gagcgatgtc gtaggcaatg tatcgggcgc gctgtgtgtg gtcggtcaca
     6181 gccggtgcca acacgtgatc gtctattttt tggccgttca catacacttc gtgataacct
     6241 accgatgcta caaatagggt tgccctggag ggtttttgtt ttaaatcgaa agattttctg
     6301 aaccacggat cagaaatttt gttgggtcct gccgaagggt catagatttc cgtggtgccg
     6361 atccattttg ctgtccattc agcatcatcg aacagcccgg tggtgaaaga cgctgattta
     6421 ctgaatgcag atgggttacc cttttcgtct tttactgcaa ctttccaaaa gtaactccga
     6481 tcagattcta gctcttttcc tttataatca acaagctgca tgttatctga agagatccat
     6541 cccgtatccc aaatattgcc cttgttttta tcgagatccc gtttcgtcga gctaaccaaa
     6601 atgcggtacg ctgtctgcgc ctggccgaaa acttgcgggt ttgcagtagc caacttccag
     6661 ctaaaccgtg gttgtcggac atccaatcct gtggggtcga caaggtattc tgtggttaat
     6721 tccataggat ataaagcacc ttgcttctgg gaaaatattg aaaaactggc tg
//
LOCUS       DAOSDR010000157         6758 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrimonas sp. isolate AMR_MDS_1826
            NODE_4111_length_6758_cov_3.856035, whole genome shotgun sequence.
ACCESSION   DAOSDR010000157 DAOSDR010000000
VERSION     DAOSDR010000157.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863355
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrimonas sp. (wastewater metagenome)
  ORGANISM  Petrimonas sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Dysgonomonadaceae; Petrimonas.
REFERENCE   1  (bases 1 to 6758)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6758)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:10:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,720
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 35
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6758
                     /organism="Petrimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4111_length_6758_cov_3.856035"
                     /isolate="AMR_MDS_1826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2023866"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..431
                     /locus_tag="PLI30_07290"
     CDS             <1..431
                     /locus_tag="PLI30_07290"
                     /EC_number="5.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008772359.1"
                     /note="catalyzes the interconversion of D-xylose to
                     D-xylulose; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="xylose isomerase"
                     /protein_id="HOI79395.1"
                     /translation="DAKQGDMLLGWDTDQFPIDLYELTQAMLVILKGGGLQGGGTNFD
                     AKTRRNSTDLEDIFIAHIAGMDAFARALEAAAGILEESPYMQMLTERYASFDSGKGKE
                     FEEGSLSLEDMREYALSLGKEPGQISGKQELYEAIVNMYI"
     gene            597..1982
                     /gene="xylE"
                     /locus_tag="PLI30_07295"
     CDS             597..1982
                     /gene="xylE"
                     /locus_tag="PLI30_07295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007760615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose transporter XylE"
                     /protein_id="HOI79396.1"
                     /translation="MKYNKTYIFGITLVATLGGLLFGYDTAVISGAEKSIEAYLIRPL
                     GLNSLIHGATVSSALIGCIIGGVISGVFSNRFGRRKTLLIAAVLFFVSALGSGFPEFI
                     FFSKGHPSIGLLLTFNFYRIIGGVGVGLASAVSPMYISEIAPADIRGRLVSFNQFAII
                     FGMLVVYFVNWGIAFGQSIDWINDVGWRYMFASEAIPAALFGMLLFLVPETPRYLVLS
                     NRDEKARSVLSRINADKLRAEAIFSEIKQSIGRTTERSRIFSYGKIVILIGILLSVFQ
                     QFVGINVALYYAPRIFESMGAAKDASMLQTIIMGFVNVVFTVVAILTVDKWGRKPLLI
                     TGSIGMAIGMFAISWLSYNEIIGISTLVFIIIYTASFMMSWGPICWVLISEIFPNKIR
                     GQAVAVAVGAQWAANYLISSTYPAMMEYSGALTYGFYGLMAVLSALFVWKMVPETKGK
                     SLEEMESLWKE"
     gene            complement(1994..3508)
                     /locus_tag="PLI30_07300"
     CDS             complement(1994..3508)
                     /locus_tag="PLI30_07300"
                     /inference="COORDINATES: protein motif:HMM:NF019011.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M81 family metallopeptidase"
                     /protein_id="HOI79397.1"
                     /translation="MKQGVTRILIFICGMFFLVSCVNRSRPTTKKVLTLGIRHESNTF
                     STLPTKESDFKVLRDKDVLKDEPWAEAGKDLGMEFIPTLHAYAWPGGVVEKRIFDSFK
                     SEILERIRKAGSLDGIYMDMHGALHVEGYEDAQAELIREIREIVGNDVLISGSFDLHG
                     NLSKDFLKHINLLTAYRTAPHRDGTETKVRAIRMLSEAMNKRLKPYIASVEIPILVPG
                     EMSITEVEPLKSIYSQIPEVSGKEGLMDVSIFAGYCWADLNRSAMRVFVVASDKKFAD
                     KANREAALLAQQIWEKRESLILDVPSGSIDEMIVKASEYDNTVFISDSGDNTTAGAPG
                     DNPQVLEALIRHNVKNTLFAGIVDEEALKQCKNTGINNEIDLVVGGKVDNVFGKPFAI
                     KGKVIYLPSDSLIGTNHESAILDVNGIKVVIMNSRRSFVTLRDFEELDINPLDFNIVV
                     VKLGYLYPELRDIAPVHLIALTSGFCNLDMRTLPFKRVTRPVYPLDLDMTWQSQ"
     gene            complement(3641..5047)
                     /locus_tag="PLI30_07305"
     CDS             complement(3641..5047)
                     /locus_tag="PLI30_07305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI79398.1"
                     /translation="MIKNKVYLKEIDWPSFGEADPIIYPSATELTERIEKCRQVMEER
                     ALTHLVVYGDREHFGNIMYLTHFDPRFEEALLLITKENDPLILVGNECVSHLSVSPLY
                     NNGSLRYERYQPFSLLDQPRDESRSLAEILSEEGIDKESRIGCIGWKYYSRLEFEHHQ
                     HVIEIPSFIVDMLRSLSSYENVINVTDMLMAPSYGLKSFLSSFEIAHFEFVNVMASEG
                     MKSLITNFKSDITDFDLAKEYRYTGYPLGCHIGMKSSGNQSIGLSSPVGALIEKGLPF
                     STGISYWGSNICRAGWVAESEHDLPEDAQDYVSNFAGPYFYACTKWLENLKIGTKGGV
                     LRQLIDEHLPFEDFGVFLNPGHLIHYEEWLSSPIYPNSEEEIHSGMYMQVDIIPRSKK
                     YSTSRMEDGVVIADSHLRNKVKEEYPEVYERCMKRRSFMIDVLGINLPEEVLPLSNIP
                     SLVPPFFLNHKKVLSLKP"
     gene            complement(5174..5246)
                     /locus_tag="PLI30_07310"
     tRNA            complement(5174..5246)
                     /locus_tag="PLI30_07310"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5211..5213),aa:Gly,seq:ccc)
     gene            5362..5435
                     /locus_tag="PLI30_07315"
     tRNA            5362..5435
                     /locus_tag="PLI30_07315"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5396..5398,aa:Pro,seq:cgg)
     gene            5573..6748
                     /locus_tag="PLI30_07320"
     CDS             5573..6748
                     /locus_tag="PLI30_07320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007848871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HOI79399.1"
                     /translation="MPNSQLPQNPLLEIISYTPPKIYTGVEWYIGFYAFDPSRGRMRR
                     KRIKINFIEKISDRRQYAKGMINRLHAQLERGWNPWIEAQHGNAYHTVKDVSDHYKRS
                     IEKLFRDGIYREDTYISYMSYIRNLEKWNEEQHVPLTYIYQFDGATVQLFLEHIYIDR
                     DNSPQTRDNYLMWLRSLSTWLVQKKYLKTKPTEGIAVFGKHSKKKKRTIISDKDMMRI
                     KDYISHKNKHYLLACYVLFYCLVRPKEMSKIKIKHISITRQTLFIPDDNSKNRKDGTI
                     TLPSKVIQLMIDLNIFNYPGEYYLFSTGFVPGKYYKNEKMFRDFWSRYVRNDLKLPAT
                     YKFYSLKDTGITSMLRKYPSLSVRDQARHSSLLTTEIYTPHDLQEADVLLKNNDGDF"
BASE COUNT         1937 a         1372 c         1422 g         2027 t
ORIGIN      
        1 tagacgcaaa gcagggcgat atgcttcttg gctgggatac agaccagttc cccatcgatc
       61 tctatgagct gacacaggcc atgctggtta tcctgaaagg cggcggactg cagggcggtg
      121 gtacaaactt cgacgccaaa acccggagaa attccacgga cttggaagat atctttattg
      181 cacatattgc cggcatggat gcttttgcac gtgctctgga agctgccgcc gggattctgg
      241 aggaatcgcc ctacatgcag atgctgacgg agagatatgc ctctttcgac agcggtaaag
      301 gaaaagagtt tgaagaaggc agcctttcgt tggaagatat gcgtgaatac gccctctcgc
      361 tgggtaaaga gccgggtcag atcagtggca agcaggaact ttatgaggcg attgttaaca
      421 tgtatatttg acggaaccaa gacgatagag ccagaaatca gaatctttgt ttcaactctc
      481 aatacaacgg tctgtgaact tatcggatac gacgttgaac aatttaacaa aaaagcctat
      541 cgggtctggc aatattttgt ctgtcctggt tcttatgtct tataatctaa ggaaaaatga
      601 aatataacaa aacatacata ttcggcatca ccctggtagc gaccctgggt ggcttactct
      661 tcggctacga cacggctgtg atttccggtg cggagaaatc gatagaggct tatctgatca
      721 ggccgttggg cttaaattcg ttaatccatg gagccaccgt atcgagtgcc ttgatcggct
      781 gtattatcgg gggagttatt tcgggagtgt tttcaaaccg gtttggcaga agaaaaacgc
      841 tgcttatagc tgctgtttta ttttttgtgt cggcattggg atctggattt cccgaattta
      901 tttttttctc caaagggcac ccttccatcg ggttgctgtt gacattcaat ttttaccgta
      961 taatcggagg tgtcggagtg ggactggcat ctgccgtttc gcccatgtat atcagtgaga
     1021 tagctcctgc agatattcgg ggaaggctgg tttcctttaa ccagttcgcc attatttttg
     1081 gaatgctggt ggtgtatttt gttaattggg gcatcgcctt tggacagagt atcgactgga
     1141 tcaacgatgt gggatggcgg tatatgttcg catcggaagc cattcctgca gccttgtttg
     1201 ggatgctgct gtttctggtt cccgaaacgc cgagatacct ggtattgtcg aatcgcgatg
     1261 aaaaagcacg gtctgtttta agccggatta acgccgataa attaagggct gaagccattt
     1321 tctccgaaat taagcagtcc atcggacgca caacagaacg ttcccgtatt ttttcttacg
     1381 ggaaaattgt cattcttatc ggtattttgt tgtcggtgtt tcagcagttt gtgggaatca
     1441 atgttgcgtt gtattatgct ccgcgtattt tcgagagtat gggggccgcg aaagatgcct
     1501 ccatgctaca aaccatcatc atggggtttg tcaatgtcgt gtttacggtt gtggccatcc
     1561 tgacagtgga taagtgggga agaaaaccgt tgttgattac cggctccatt ggcatggcta
     1621 tcggtatgtt tgccatatcc tggttgtcct ataatgagat tatcggaata agcacgctgg
     1681 tatttatcat tatctacacg gcgtcgttta tgatgtcgtg gggaccgatc tgttgggtgc
     1741 tcatctctga aatttttcca aacaaaatcc ggggtcaggc ggtcgccgtg gcggttggcg
     1801 cacaatgggc tgccaattac ctgatttcgt ctacctatcc ggcaatgatg gaatatagcg
     1861 gagctttgac ctacggtttt tacgggctga tggctgtact ttcggctctt ttcgtttgga
     1921 aaatggtccc cgaaaccaaa ggaaaatcac tggaagagat ggaaagcctg tggaaagagt
     1981 gagcttgaat tacttattga gattgccagg tcatatccaa atctaaagga tatacgggtc
     2041 tggttacacg tttaaagggt aatgttcgca tatccagatt gcagaacccc gatgttaatg
     2101 ctattagatg tacaggagcg atatccctta attcaggata aagatatccc aacttgacaa
     2161 ctacaatatt gaagtctaat gggttaatat ctaactcttc gaaatctctc agcgttacga
     2221 atgagcgtcg ggaattcatg ataacaactt taataccgtt gacatcaagt atggcggatt
     2281 catgattggt acctattaat gaatccgatg gtaaataaat gactttccct tttatggcaa
     2341 aaggtttacc aaagacatta tccacttttc ctccgacaac taaatctatt tcgttgttaa
     2401 tgcctgtatt tttacattgc ttcaaggctt cctcatcaac tattccggca aataaagtgt
     2461 tcttgacatt gtgcctgatt aaagcttcta gtacttgtgg attatcccct ggagctccgg
     2521 cggtagtatt gtctcctgag tcggagatga aaactgtatt gtcatactct gaagctttta
     2581 caatcatctc atcaatagaa cctgaaggga catcgagtat tagactttcg cgtttttccc
     2641 atatttgttg agccaataga gctgcttcac gattcgcttt gtcggcaaat tttttatcgg
     2701 atgcaactac aaagacccgc atggctgagc gattgagatc ggcccagcag tatcctgcaa
     2761 aaattgaaac atccattaat ccttctttcc cactaacttc gggtatctgt gaatagatcg
     2821 acttcaacgg ctcaacctct gtaatactca tttctccagg aactaaaata ggtatctcaa
     2881 cactggctat gtaaggcttg agccgtttat tcatagcctc agaaagcatc cgtatggcac
     2941 gtacctttgt ctcggtaccg tctctgtgag gggcggtcct ataagctgtc aggagattaa
     3001 tatgctttag gaagtccttt gataaatttc cgtgtaaatc aaaactgccg gaaatcaata
     3061 catcatttcc aactatttcc cttatttctc tgatcaactc tgcttgtgca tcttcatagc
     3121 cttcaacgtg aagggctccg tgcatatcca tataaattcc gtctaatgaa ccggctttac
     3181 gtattcgttc caatatttct gacttgaacg aatcaaatat tcttttttca acaactccgc
     3241 ctggccaggc ataggcatgt agagttggga taaactccat acctaaatcc tttcccgctt
     3301 cagcccatgg ttcatctttt aaaacatcct tatcccgcaa caccttaaag tctgactctt
     3361 tggtgggtaa agtggagaat gtgttcgact cgtgacgaat accgagtgtt aaaacttttt
     3421 tagttgtggg tcttgaacgg ttaacacagc taaccaaaaa gaacattcca catataaaaa
     3481 ttaaaatacg tgttactcct tgtttcatgc ttcagttttt ctctttgata aaatgcctat
     3541 taattcaact aaatgattca tgagcgatct tcttgtttaa aagatattgt aataactgtt
     3601 gttataagga acagttttat atattcctat cttttatcaa tcaaggtttt aaacttaata
     3661 cttttttgtg attaagaaaa aacgggggga ctaaagaggg tatattggaa agaggcagaa
     3721 cttcttcggg caaatttata cccagtacat caatcataaa ggaacgtcgt ttcatacacc
     3781 tctcatacac ttcgggatat tcttctttta ccttgtttcg gagatgactg tctgcaatta
     3841 caaccccgtc ctccattctt gaggttgaat atttctttga ccggggtata atatcaacct
     3901 gcatgtacat gcctgaatga atctcttctt cagagttagg ataaatggga gaacttaacc
     3961 attcttcata atgaatcaaa tgacccgggt tcagaaaaac tccaaagtct tcaaaaggta
     4021 aatgttcatc aatcaattgg cgcagaacac ccccttttgt cccaattttc aaattttcca
     4081 accatttagt gcaggcatag aaatagggcc ctgcaaaatt cgaaacatag tcttgtgcat
     4141 cttccggaag atcatgctcg ctttcagcaa cccatccagc cctgcaaata ttacttcccc
     4201 aataggaaat tcctgtagaa aagggcaatc ctttttcaat caatgctcca actggactgg
     4261 ataacccaat actttgattt ccactacttt tcatgccaat atgacatcct aacgggtagc
     4321 ctgtgtaccg gtattccttt gccaaatcaa aatcggttat atctgactta aaattggtta
     4381 ttaaggattt cattccttct gatgccatta cattaacaaa ttcaaaatgt gcaatttcga
     4441 aagatgaaag gaaagacttt aatccgtagg agggggccat taacatatca gttacattga
     4501 ttacgttttc gtaactgctc aaagaacgga gcatatctac tataaacgaa ggtatttcaa
     4561 taacgtgctg atgatgttca aactccagtc ttgagtaata tttccaaccg atgcacccga
     4621 ttctcgattc tttgtctatt ccttcttctg aaaggatttc agcgagtgaa cgactttcat
     4681 ctctgggttg atccagtaga gagaatggct ggtacctttc gtaccttaaa gaaccgttgt
     4741 tgtataatgg agatacgctt aaatgactca cgcactcatt accaactaga atcaatggat
     4801 cattttcttt ggttattaac aacaatgctt cttcaaaacg aggatcaaaa tgagtcaggt
     4861 acattatatt gccaaaatgt tctctatcac catacacaac caggtgtgta agcgctcttt
     4921 cctccatcac ttgccggcat ttttcaattc tttctgttaa ctccgttgct gatggataaa
     4981 taatcgggtc agcttcacca aaactgggcc aatcgatttc ttttaaatat accttatttt
     5041 ttatcatggc agcgtttttt ataagttatt gattttaaga gtattggttg aactgtaacg
     5101 atttgctctt acactgtttt ccccaaagga aaagcaccta ccggagaatc cgataggtgc
     5161 atgatttcca gagagcggca aacgagattc gaactcgcga ccctcagctt gggaagctaa
     5221 tgctctaccg actgagctat tgccgcaaat agttatgcaa atgtaacatt tttttcgatc
     5281 gatttattca ttgggcaaga gtttttttca cttttgttcc gaacgaacaa aaaaatattg
     5341 tacctttgca tacgtaaaaa tcgggatgta gcacagttgg ctagcgcgcc acgttcggga
     5401 cgtggaggtc gggtgttcga gtcacctcat cccgactcat ttacgccgat atcttcactg
     5461 gcaaacgata agcgatgatg tcgcatgtat cagccgggac agatacggga cagtaacaga
     5521 aaaatcattt ttacgcttcc gaaataccaa aatttcggag gtgtaaaaaa aaatgccaaa
     5581 ttcacaacta ccccaaaatc cgctattaga aattatatcg tacactccac caaagatata
     5641 tacgggagtg gagtggtata tcggatttta tgcgtttgat ccgtcgcgtg gaagaatgcg
     5701 ccggaagaga atcaaaataa attttatcga aaagatatca gaccggaggc agtatgcaaa
     5761 aggaatgata aatcggcttc atgcacaatt ggaacgcgga tggaatccgt ggattgaagc
     5821 tcagcacgga aatgcttatc acactgtaaa ggatgtatct gatcattata agcgttcgat
     5881 tgaaaaatta ttcagggatg gaatttaccg ggaagatacc tacatatctt acatgtcgta
     5941 tattcgtaat cttgaaaaat ggaacgaaga gcagcatgta cccttaacat acatatacca
     6001 atttgatggt gcaaccgtac aattgttcct ggagcacata tatatagata gagataattc
     6061 accgcaaacc cgcgacaatt atttaatgtg gttgagaagc ttaagcacgt ggttagtaca
     6121 aaagaaatat ttgaaaacga agccaacgga aggcattgca gtttttggga agcattcaaa
     6181 aaagaaaaag cggacaatta tttccgataa agacatgatg agaattaagg attatatctc
     6241 gcataaaaac aaacattacc tgctggcctg ttatgttttg ttttattgtt tagtacggcc
     6301 caaagaaatg agcaaaataa agataaaaca tatttctatt accaggcaaa cattgtttat
     6361 cccggatgat aattccaaga atcgaaaaga cgggacgata acgcttccgt caaaggttat
     6421 tcaattaatg attgatttga acatctttaa ttaccctggc gaatattatc tgttttcaac
     6481 cggatttgta cccggtaaat attataaaaa tgaaaagatg ttccgcgatt tctggtctcg
     6541 atatgtaaga aatgatttaa aactgcctgc aacttataaa ttttactcgc tgaaagatac
     6601 gggaataaca tcgatgctca ggaaatatcc gagcctgagt gtgagggatc aggcccgaca
     6661 ttcatcttta ttaactactg agatatatac tccgcacgat ttgcaggaag cggatgtgtt
     6721 gcttaaaaat aatgacggag atttttagga gtttaacg
//
LOCUS       DAOSDR010000158         6727 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrimonas sp. isolate AMR_MDS_1826
            NODE_4136_length_6727_cov_3.860612, whole genome shotgun sequence.
ACCESSION   DAOSDR010000158 DAOSDR010000000
VERSION     DAOSDR010000158.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863355
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrimonas sp. (wastewater metagenome)
  ORGANISM  Petrimonas sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Dysgonomonadaceae; Petrimonas.
REFERENCE   1  (bases 1 to 6727)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6727)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:10:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,720
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 35
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6727
                     /organism="Petrimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4136_length_6727_cov_3.860612"
                     /isolate="AMR_MDS_1826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2023866"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..533)
                     /locus_tag="PLI30_07325"
     CDS             complement(<1..533)
                     /locus_tag="PLI30_07325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019539268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopentenyl transferase family protein"
                     /protein_id="HOI79400.1"
                     /translation="MNTLLVLLGPTGVGKTEVSLQIAERLNSPVISSDSRQIYKQMVI
                     GTAAPTNDQLGRIPHYFVGTLEPEDYYSASQFEEDAVPLIENLLQTHSFVVMSGGSMM
                     YIDAVCQGIDEIPTIDEKLRKEIHDLYLKEGLDAIRSRLKILDPVFYNQVDLKNPKRV
                     IHALEVCLMAGRPYSSLR"
     gene            complement(591..1148)
                     /locus_tag="PLI30_07330"
     CDS             complement(591..1148)
                     /locus_tag="PLI30_07330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006843218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI79401.1"
                     /translation="MIFRFAIISDEVENFKREIKIDADNTFLDLFKAIVDCTGFNESE
                     MASFFLCDDNWRKEQEITLVEMDTYSDEDPYTMAECVLNDYLEDEKQKLLFVFDYLTE
                     RALFIELSEIITGKSLKKPICTLSVGEAPKQFLNFDEIASTSASLDVDESFFGDESFD
                     LNELDKEGFDGLDDIEISAEESDLY"
     gene            1332..1405
                     /locus_tag="PLI30_07335"
     tRNA            1332..1405
                     /locus_tag="PLI30_07335"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1366..1368,aa:Asn,seq:gtt)
     gene            1439..1512
                     /locus_tag="PLI30_07340"
     tRNA            1439..1512
                     /locus_tag="PLI30_07340"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1473..1475,aa:Asn,seq:gtt)
     gene            1630..2583
                     /locus_tag="PLI30_07345"
     CDS             1630..2583
                     /locus_tag="PLI30_07345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006841823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HOI79402.1"
                     /translation="MKKILITTALHRESLAELFEKFEVFMPQDKDMKRDQVLEMISDY
                     EVVIPNFSFFTDKEIMDRGVRLEVIANYGVGYNNIDVEYATQKGIVVTNIPKTTCEPT
                     AELAFALLLAAGRRIGYYDRKLREHRAVDWGVYGDAGLPIFGKTLGIIGMGRIGQALA
                     RRAVASGMNIIYHNRNRLSPEIEERYGASYVSFDELLRMADFISLNAPSTSQTLKLIG
                     EKEFSVMKPTAVFINTARGNMVDEKALAQALKEKKIWAAGLDVYENEPKISSELLVLD
                     NVVLSPHAGTRTIEDRHRMAEEMVHNIIGFYEGKYEVSRVN"
     gene            2632..3342
                     /locus_tag="PLI30_07350"
     CDS             2632..3342
                     /locus_tag="PLI30_07350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005860462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HOI79403.1"
                     /translation="MTAESVKQQVFSFGNPQKAEHSKYFFKTGKGQYGEGDRFIGSTV
                     PETRKVAKANKNLSFDDLGKLLNDEYHECRFCALVILSEQFEKACDTGRKEIFEFYLA
                     HTHGINNWDLVDVSAYHIVGEWLLDKDRSVLYQLASSENLWEQRIAIISTLAFIRKKD
                     FTDTLKLAEKFLTHRHDLMHKACGWMLREAGKRDEKTLTCFLDAHYTEMPRTMLRYAI
                     EKLTPEQKKKYMMKEVQN"
     gene            3358..4578
                     /locus_tag="PLI30_07355"
     CDS             3358..4578
                     /locus_tag="PLI30_07355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008156214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HOI79404.1"
                     /translation="MFQTHHLTNGLRIIHHPFPSEISYCGIAINTGSRDEFPDEQGMA
                     HFVEHMLFKGTEKRQAHHVINRMENVGGELNAYTTKEETFIYATFLSEYFERAVELLS
                     DMVFHSTFPEHQIEKERDVILDEMNSYADSPSELIFDDFENLLFPNHEIGHYILGTSE
                     SLLALDKKKVDDFVNRQYSPANMVLFSFGKTPFSKVVRLTEQYFGFPDTRYLLSKKRE
                     RPLSETAKKSRIGKGTAQTHVILGARTFDMFQPDRYALYLLNHIVAGGATNSRLNNSL
                     REKNGLVYNVESNVTLYTDTGLFSVYFACDKRQVERCLKLINKELRRIIEIPLSSGQL
                     ATAKRQYKGQLGIASENNESISLRMAKSYLHFNHYLPLEEVFSQIDAISAVQLQELAE
                     KLFFPEQLYQLKYV"
     gene            4849..5475
                     /locus_tag="PLI30_07360"
     CDS             4849..5475
                     /locus_tag="PLI30_07360"
                     /inference="COORDINATES: protein motif:HMM:NF024895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin family protein"
                     /protein_id="HOI79405.1"
                     /translation="MKKLRLSLVVAMLAIVTAASAQLNLGIKGGVNMSNFYGDELTDK
                     NMKIGFHVGLAADYDFAPSMAIQTGLFFTTKGAKYSGSLGTVSGDITVNPMYLQLPVH
                     FAYKQEVTPGTRIVFHAGPYVAYGVGGKSKLKVSSGSSSTESEGVNVFGENKMLKPFD
                     AGLGLGVGAEFSRFLVDVGWDMGLVNISNSSSGNVKNQNAYLSVGYKF"
     gene            5667..6371
                     /locus_tag="PLI30_07365"
     CDS             5667..6371
                     /locus_tag="PLI30_07365"
                     /inference="COORDINATES: protein motif:HMM:NF024895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin family protein"
                     /protein_id="HOI79406.1"
                     /translation="MKKIVLALAMVAMVGAVSAQGLSLGIKGGVNMSNLYGKEVKDTK
                     MKLGFNVGIAADYEFESNVAIQSGLYFITKGAKLSSTKIDQKVGDIKLSGTVNKTANA
                     MYLQIPLHLAYKIDVTPGARVVLHAGPYAAYGVGGKISGKSTVKVSGNVPADQKAAVD
                     AALKQINASTNSVNTFDKKMGYKPFDAGVGIGVGAEFGSFLVDLGWDMGLLNISRNKE
                     VNVKSQNAHLAVGFKF"
     gene            6539..>6727
                     /locus_tag="PLI30_07370"
     CDS             6539..>6727
                     /locus_tag="PLI30_07370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI79407.1"
                     /translation="MKRFLLLYLSLCSVWLSYSQVGLQQLLNNAALKHASVGIQVTDL
                     NTGKTIVSHDPQKSLTPAS"
BASE COUNT         2014 a         1271 c         1494 g         1948 t
ORIGIN      
        1 cgcagtgatg aatatggcct tcctgccata aggcatactt cgagcgcatg aattacccgc
       61 ttgggatttt tcaagtcgac ctggttgtaa aaaaccggat ccaagatttt caggcgggaa
      121 cgaatagcat ccaacccttc tttcaggtaa aggtcatgaa tttcttttcg taatttttcg
      181 tcgatggtcg ggatttcgtc aatcccttga caaacagcat cgatgtacat cattgagcca
      241 ccagacatca ccacaaacga atgggtttgc agaaggtttt cgataagcgg aacagcatct
      301 tcctcaaact ggctggcact gtaataatct tcaggttcaa gagttcctac aaagtagtgg
      361 ggaatacgtc ctagttgatc gttggtaggc gccgccgtac cgatgaccat ttgtttgtat
      421 atttgcctgg aatctgagga tattacgggg ctattcaacc gttcagctat ctgaaggctt
      481 acttcagttt tgcccacgcc tgtaggtccc agaagcacga gcaaagtgtt cataaaacag
      541 gtagtttgtt gcggttcagg aagatgatta tttcgtcaaa acaagtgaag tcaatagagg
      601 tcgctttctt ctgctgatat ctcaatatcg tctaacccgt cgaagccctc tttgtcgagt
      661 tcgttcaaat cgaaactttc atctccgaaa aaggactcat ctacgtccag cgatgccgag
      721 gtgcttgcga tctcatcgaa attaaggaat tgtttgggcg cttctcctac ggagagtgta
      781 catatcggtt ttttcaagct tttaccggta ataatctccg agagctcaat aaacaatgct
      841 ctctcggtaa ggtagtcaaa tacgaaaagc aatttttgtt tttcatcttc caagtagtcg
      901 ttcagcacgc attcagccat cgtataaggg tcttcatcgg aataagtgtc catttccaca
      961 agagtgattt cctgctcttt acgccagttg tcgtcacaaa gaaagaaaga agccatctcg
     1021 ctttcattga aaccggtgca gtcgacaatc gctttgaata aatccaagaa agtattgtcg
     1081 gcatctattt ttatttctct tttgaagttt tctacttcat ccgatataat tgcgaatctg
     1141 aaaatcataa aaaagcgttt taatactcgt ttatttgatt tcaaaggtaa taaaaacttt
     1201 tttaaataaa agatgattta tctacaaaaa cttgtgttga aaacatctcc gataatttta
     1261 tgacaaagct gttggaatat ttgaaaattt gtagtacttt tgcatcactt ttctgaaaag
     1321 aaatataaaa ttcttcagta gctcagtcgg ttagagcatc tgactgttaa tcagagggtc
     1381 gctggttcaa gtccagcctg aagagcgaaa aataacgtta gaaaaagaga agtgaaattc
     1441 ttcagtagct cagtcggtta gagcatctga ctgttaatca gagggtcgct ggttcaagtc
     1501 cagcctgaag agcgaaaaga gagtgggtag taatgctcac tcctttttct tttagcgtgt
     1561 atgatatttt cagaatgtcc ctcgtaaaca attttgcgca acgctcgttt aatgaaaaac
     1621 aatccgatca tgaagaaaat tcttataaca actgccctgc acagagaatc tttagcagaa
     1681 ctttttgaga agtttgaagt ttttatgcca caggataaag acatgaaacg cgaccaggta
     1741 ctggaaatga tttctgatta tgaggttgta atacctaact tcagtttttt taccgataag
     1801 gaaataatgg acaggggagt gaggttggag gtgattgcta actacggtgt cggttacaat
     1861 aatattgatg tggaatacgc aacccagaaa ggaattgtgg taaccaacat tcccaaaacg
     1921 acgtgcgaac ctacggctga attggcattt gccttgttgc ttgccgcagg aaggcgaatt
     1981 gggtattatg accggaagct acgcgaacac cgggcggttg actggggtgt gtacggagat
     2041 gccggattgc ctatttttgg aaaaaccttg ggtataatcg gaatgggaag aatcggacaa
     2101 gccctggcgc gacgtgcagt agcttcaggg atgaatataa tctatcataa ccggaaccgg
     2161 ttaagtccgg aaattgaaga acgttatggt gcttcatacg tctcttttga tgagttactg
     2221 cgaatggcag attttatttc actgaatgca ccttccactt ctcaaacctt aaagttgatc
     2281 ggagaaaaag agttttccgt aatgaaacct acagcggtat ttattaatac agcccgagga
     2341 aatatggtcg atgaaaaagc attggctcaa gcgctgaaag aaaaaaaaat atgggctgcc
     2401 gggcttgatg tatatgaaaa tgaaccaaaa atctcttccg agttactggt gttggataat
     2461 gtggtacttt cgcctcatgc cggaaccaga accattgaag accgtcatcg gatggcggag
     2521 gaaatggtgc ataatattat tggtttttac gaaggcaaat acgaggtgtc ccgggtaaat
     2581 tgatggacgt agagttttga acacaatacc ctacgctaaa ttctaattag catgacagca
     2641 gaaagtgtaa aacaacaggt gttctctttt ggaaatcccc aaaaagcaga acattccaag
     2701 tattttttca agaccggaaa aggtcagtac ggtgaaggcg accggtttat aggaagtact
     2761 gtccccgaaa cgagaaaggt ggctaaagct aacaaaaacc tttcgtttga cgacctcgga
     2821 aaactgttga atgacgagta tcacgaatgt cgtttttgtg ctttggtcat tctgtctgag
     2881 caatttgaaa aagcctgcga cacggggcgt aaagaaattt ttgaatttta ccttgcccat
     2941 actcacggta tcaataattg ggacctggtg gacgtgtcgg cttaccatat tgtaggggag
     3001 tggcttctgg acaaagacag gtcggtcctg tatcaactgg ccagcagtga aaacctctgg
     3061 gaacaacgga tagccattat ttccacattg gcctttatcc gaaaaaaaga ttttacagat
     3121 acgttgaaac ttgctgaaaa gtttcttacc catcgtcacg atttaatgca caaagcctgt
     3181 ggctggatgt tgcgtgaagc cggtaaacgc gatgaaaaaa cattaacctg ttttctggac
     3241 gcacattata cggaaatgcc ccggacaatg ctgcgatacg ccatcgagaa gttaacgccg
     3301 gagcagaaaa aaaagtacat gatgaaagaa gtccagaatt aacacccatt atcgaacatg
     3361 tttcaaaccc atcacttaac caacggctta cgcattattc atcacccttt cccctcggaa
     3421 atatcctact gtggaatagc cattaatacg ggttcccgtg acgaatttcc ggacgaacag
     3481 gggatggcac attttgtgga gcatatgttg tttaaaggaa ctgaaaagcg ccaggcacat
     3541 catgtcatta accgcatgga gaacgtgggc ggcgaactta acgcttatac cacaaaggaa
     3601 gaaacgttta tttacgctac gtttttatcc gaatattttg agagagcagt ggagttgtta
     3661 agcgatatgg tttttcattc cacttttccc gaacatcaga tagaaaaaga aagagacgtt
     3721 attttagatg aaatgaatag ttatgccgac tcaccgtctg agttgatatt tgatgatttt
     3781 gaaaatctct tgtttcctaa tcacgagata gggcattaca tactgggaac atctgagtcg
     3841 ttacttgctt tggataagaa aaaagtggac gattttgtaa acaggcagta ctcccctgcg
     3901 aatatggttt tgttttcatt cggaaaaact cctttttcca aagtggtacg cttgactgaa
     3961 caatactttg gtttccccga tacccggtat ttattatcca aaaagcgcga acgtcccctg
     4021 agcgaaacag caaaaaagag ccggattgga aaaggtaccg cccaaacgca tgttatcctc
     4081 ggagcccgca cttttgatat gtttcaaccg gacaggtacg ctctttattt gttgaatcat
     4141 attgttgccg ggggagctac caacagccgg cttaacaatt cgttgcgtga aaaaaacgga
     4201 ttggtctata acgtggaatc gaacgtaacg ttgtacaccg atacggggct gttttccgtc
     4261 tattttgcct gcgataaaag acaagtggaa cggtgtttaa agcttatcaa taaagagtta
     4321 agaaggatta tagagattcc tttgtcctca ggtcaactgg caactgcaaa aagacaatat
     4381 aaaggacagc tgggcatcgc ttctgaaaac aatgaaagta tctccctgag aatggctaaa
     4441 agctatcttc attttaatca ttaccttcct ttggaagagg ttttttccca aatagatgcc
     4501 ataagtgcag tgcaactgca ggaattggca gagaaattgt tcttcccgga acaactgtat
     4561 cagttgaaat acgtgtgatt taagtagtct ttatccctaa caaacatcaa aaaagcatca
     4621 ataggcagaa tttttgttaa aaacctgttg agaaattcca cacagttccc attctcattt
     4681 tgtggctgtt ttaatctgga ttacatttga tgcatgcttt ttgttaagca tccgttgaaa
     4741 aattcaaaac cgaacaaaaa aaacaaaacg gttgtttatt aagcgtatca gaagtgtaat
     4801 gaacattgtt tttgaacagg ctatatttat taattaaaca attaaattat gaaaaaatta
     4861 agattatcgt tagtggttgc catgctggct atagtaacgg cagcaagtgc acaattgaat
     4921 ctggggatta aaggcggcgt taatatgtcg aatttttacg gtgatgaatt gaccgacaaa
     4981 aatatgaaaa ttggttttca tgtcggtcta gctgcagatt atgactttgc cccgagcatg
     5041 gctattcaaa ccggattatt tttcacgacg aaaggggcta aatacagtgg ctctctggga
     5101 accgtttcag gagacattac tgtaaatccg atgtacttac aactccccgt tcacttcgct
     5161 tataaacagg aggtgactcc gggtacccgt attgtatttc acgcaggtcc ttatgtcgca
     5221 tacggggttg ggggaaaatc gaaacttaaa gtatcgtccg gtagtagttc aacagaaagt
     5281 gagggcgtta atgtttttgg agaaaacaaa atgcttaaac cgtttgatgc cggtttagga
     5341 cttggcgttg gtgcagaatt cagtcgcttt ctggttgatg tcggatggga tatgggtctg
     5401 gttaatatat ccaattcaag tagtggaaac gtaaaaaatc aaaacgccta cctctccgta
     5461 ggttacaaat tctaattttt cagaattata aacgttggaa acaccgcttt ttgggcggtg
     5521 tttttttatt gtaagcagta atcttttgtg aattttttca accgatagtg agggtttttt
     5581 gtttccctgc gtcttattat cggaagtgag aaacaacatc acttcgttga atttttaaac
     5641 aaactaacta ttaatttaaa aaagttatga aaaagattgt attggcattg gccatggtgg
     5701 ccatggttgg agctgtgagc gctcagggat tgtctctagg aattaaaggc ggagtaaaca
     5761 tgtcgaatct ttatgggaaa gaggtaaaag acaccaagat gaaattaggt tttaacgtgg
     5821 gtatagcagc tgattatgaa tttgaatcca atgtggctat tcagtccgga ttgtatttta
     5881 ttacgaaagg agcaaaacta tcttccacaa aaatagatca gaaagtagga gacattaagc
     5941 tttcgggtac ggtaaataaa acagccaatg ccatgtattt gcagatccct ctgcaccttg
     6001 cctataaaat agatgtaacc cccggggcgc gtgttgtgtt gcatgccggt ccctatgcgg
     6061 cctatggagt tggaggcaaa ataagcggta agtcaactgt aaaagtttca ggcaatgtgc
     6121 cggcagatca aaaagcagca gtcgacgctg cccttaaaca gataaatgct tctacaaaca
     6181 gtgttaatac ttttgataag aaaatggggt ataagccttt cgacgctggt gtcggtatag
     6241 gtgttggtgc cgaattcggt tctttccttg tcgatttggg atgggatatg ggcttgctca
     6301 atatttctag aaataaagaa gttaacgtaa aaagtcaaaa tgcgcacctt gcagtaggtt
     6361 tcaaatttta agaagacctg tttaaatagt gattacaaag acaccgcttt cagtaaggcg
     6421 gtgtcttttt tgcgctgtgc acgacaatta attatttttg acgattaagg atgaattgtt
     6481 gtagagataa accagtttgt cttttttttc ttatttttgt ccgtactaaa aaaaacgaat
     6541 gaaacgattt cttcttttat atctttcact gtgttccgtt tggttatcct attcgcaggt
     6601 tgggctgcaa caactcctca ataatgccgc cttgaagcat gcctcggtag gtattcaggt
     6661 cacggacctg aataccggga aaacaattgt ttcgcacgat ccgcaaaaat cgcttacgcc
     6721 ggcatcg
//
LOCUS       DAOSDR010000159         6693 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrimonas sp. isolate AMR_MDS_1826
            NODE_4158_length_6693_cov_4.261976, whole genome shotgun sequence.
ACCESSION   DAOSDR010000159 DAOSDR010000000
VERSION     DAOSDR010000159.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863355
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrimonas sp. (wastewater metagenome)
  ORGANISM  Petrimonas sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Dysgonomonadaceae; Petrimonas.
REFERENCE   1  (bases 1 to 6693)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6693)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:10:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,720
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 35
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6693
                     /organism="Petrimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4158_length_6693_cov_4.261976"
                     /isolate="AMR_MDS_1826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2023866"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            114..926
                     /gene="bamD"
                     /locus_tag="PLI30_07375"
     CDS             114..926
                     /gene="bamD"
                     /locus_tag="PLI30_07375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014225848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0065003 - protein-containing complex
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HOI79408.1"
                     /translation="MRVKPVYFLLLAVLLTSCGEYNKILKSTDKDVKYTYAKKYFEEG
                     KYSRSITLLEELVPFLKGTASAEESLYLLGQSYYNSKDYLSATEMFTTYYNTYPKGEY
                     AEPALFYAAYGLYLDSPDSKLDQSKTYKAIAEFQRYMEEYPQSERSEQAKNYMFELQE
                     KLAHKELLAAQLYLNLGNYMGNNYESAVITAREAMKSYPFSKYNEEYQMIILRARFEY
                     AERSTLGTQPERYRMVLDEYFNYKNTFPNGKYMPEADRYYKKAQSKIDVLPS"
     gene            974..1306
                     /locus_tag="PLI30_07380"
     CDS             974..1306
                     /locus_tag="PLI30_07380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007658146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HOI79409.1"
                     /translation="MDYRRTNAPQNTETRDVMKLAEPVGNVYEMVRIISKRANQLSVE
                     MKQDLDTKLQEFASYTDNLEEVFENHEQIEISRFYEKLPKPSLIALQDWKEGEIYYRN
                     PSKEKEQF"
     gene            1316..1771
                     /locus_tag="PLI30_07385"
     CDS             1316..1771
                     /locus_tag="PLI30_07385"
                     /inference="COORDINATES: protein motif:HMM:NF025491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4293 domain-containing protein"
                     /protein_id="HOI79410.1"
                     /translation="MLQRIQTLFLLLASVCMLVATVTPLASFFYNGDPVVFEAMGMYR
                     SGDLESSTWALFAIGAISALLPIVTVFLYKTRILQIRISIFNIFLMVGFYLYFGFLIF
                     NINPEAGLQFQKIGIGAIMPVIAIILTYLAIRRIGADEILVRSFHRLRK"
     gene            complement(1775..3211)
                     /gene="aspA"
                     /locus_tag="PLI30_07390"
     CDS             complement(1775..3211)
                     /gene="aspA"
                     /locus_tag="PLI30_07390"
                     /EC_number="4.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010265966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008797 - aspartate ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate ammonia-lyase"
                     /protein_id="HOI79411.1"
                     /translation="MNKTTSKFSRDTRTEHDLIGAMEIPTASYFGIQTLRAVENFDIS
                     RSKLHHYPQLIRGLADVKAAAALANKELELLNPAIADAIVEACREIKHGKLHDQFVVD
                     MVQGGAGTSTNMNANEVIANRALEILGFEKGDYTHCHPNNHVNLSQSTNDAYPTALKI
                     ALYRSIGELINALEKLIVVFREKGKEFAEVIKMGRTQLQDAVPMTLGQEFDAFATTLE
                     EEVSRLSQNRQLLLETNMGATAIGTGINSDPEYTVICTRYLAEISDIAVVAADNLIEA
                     TSDTGVFIMNSSVIKRLAVKLSKICNDLRLLSSGPRTGLNEINLPPMQPGSSIMPGKV
                     NPVIPEVVNQIAYKVIGNDLTVTMASEAGQLQLNVMEPIIAYSLMENIEMLINGINTL
                     RSKCISGITANQDRCRDMVLNSIGLVTALNPYLGYEDSTRVAAEALRTGKGVYDIVLE
                     NKLIAQEELDEILKPENMIRPRRINKLK"
     gene            complement(3409..4320)
                     /locus_tag="PLI30_07395"
     CDS             complement(3409..4320)
                     /locus_tag="PLI30_07395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019539713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HOI79412.1"
                     /translation="MERKKLNQPVKFSLVMVIALALLQGCGSVPFTGRRQLSLVSNEQ
                     VVALSLQQYQDFMRSAPLERSTTNAEMVKRVGSRIANAVETFYKNNGYESELQYYQWE
                     FNLVKDKSVNAFAMPGGKIVIYDGLLPVTQTEEALAVVVGHEVAHVIARHAQERISQQ
                     MALQYGGAVAGGLLGNSVGAQIGQQVFGLGAQFGVMMPYARKQEYEADEIGLIVMALA
                     GYNPQAAVPFWTRMAQSSQGGAPPEFLSTHPTDEKRIARIQQIMPNVQQYYKGTGVQN
                     TTSEPIKTQAPVPAKTEEAKTSKEWTF"
     gene            4586..>6693
                     /locus_tag="PLI30_07400"
     CDS             4586..>6693
                     /locus_tag="PLI30_07400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005863421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase/topoisomerase IV subunit A"
                     /protein_id="HOI79413.1"
                     /translation="MSLKDSNEEDFEDKDMQMNPEEDDILLSDSSQHSNPRIITRLAE
                     DSPMNLSRMYQDWFLDYASYVILERAVPHVTDGLKPVQRRILHAMKRMDDGRYNKVAN
                     IIGFTMQFHPHGDASIGDALVQLGQKDMLIDCQGNWGNVHTGDGAAAPRYIEARLTKF
                     ALEVLFNAKTTEWKPSYDGRNKEPVTLPAKFPLLLAQGTEGIAVGLTSKILPHNFNEL
                     CDASVAYLQGKKFSLFPDFQTGGYIDVEKYNDGERGGSVKVRASIKKLDNKTLVITSL
                     PYGKTTASVVESIIKANDRGKIKIRKVDDITAANVEIHVQLAPGVSSDRTIDALYAFT
                     DCEINISPNCCIIEDDKPHFLTVSDVLRISTDRTKELLRLELEIEKQEKQEALLFASL
                     EKIFIEERIYKDNEFENARNMEIAVAHIDKRLEPYKPSFIREITSEDILRLMEIKMAR
                     ILKFNSDKAEENIARFRGEIEEINHHLDNLTDYTISWYLMLKEKYGKNYPRRTEIRSF
                     ENIEAVKVAEANEKLYINREEGFIGTGLKKDEFVCNCSDIDDIIVFFKDGKYKVVKVS
                     DKMFVGKGILYANVFKKNDKRTIYNVIYRHGKSGAHYIKRFPVTGVTRDKDYDVTQGK
                     TASRIVYFSANPNGEAETVRVILKPKPRQRILQFEKDFSEIAIKGRGAMGNILTKADV
                     HRIMLKQKGLSTLGGRKVWF"
BASE COUNT         1849 a         1454 c         1524 g         1866 t
ORIGIN      
        1 aagtattttg tccgtgttgg gtataaaatc ctaaaataga ataaaattgg ctttcgatga
       61 actaaaactg agaaagtttc cgtaaatttg tgcgctaaaa tcgattttac gaaatgagag
      121 taaaacctgt atatttttta ttgcttgctg tgttgctgac ttcgtgcggc gaatacaaca
      181 agattttgaa aagtaccgat aaggatgtga aatacacata cgcaaagaag tatttcgaag
      241 aaggtaaata cagtcgctcc attaccttac tcgaggaact ggtgcccttt ttaaagggga
      301 cagccagcgc agaggaatcg ttatatttat tggggcaaag ttactacaat tcaaaagatt
      361 atctctctgc tacagagatg tttactactt actacaatac ataccccaag ggagagtatg
      421 ctgagccggc cttgttttat gcagcttacg gtttatatct cgattctccc gattccaagc
      481 tggatcaatc taaaacatat aaggccattg ccgagtttca gcgctacatg gaagaatatc
      541 ctcaaagtga gcgatcggaa caagccaaga actatatgtt tgagttgcag gagaagttgg
      601 ctcataaaga gctgttggct gcgcagttat acttgaacct gggaaattat atgggtaaca
      661 attacgaatc ggcagtcata actgctcgtg aagcaatgaa aagctacccg ttttcgaaat
      721 acaacgagga gtatcagatg attatcctcc gtgcccgttt tgagtatgcc gaacgcagta
      781 cattagggac ccagcccgaa cgataccgta tggtgcttga tgagtatttc aattacaaaa
      841 atactttccc aaacggaaaa tacatgccgg aagctgacag atactacaag aaggcgcaaa
      901 gtaaaataga cgtgctgccg tcataaatca attaattgaa cacaaggaat ataactaaga
      961 attagcatat aaaatggatt acagaagaac aaatgcacct cagaacaccg aaacccgtga
     1021 tgtgatgaaa cttgccgagc cggtggggaa tgtctatgag atggtccgca taatcagtaa
     1081 aagagctaat cagctttctg tcgagatgaa gcaggacctg gataccaaat tgcaggaatt
     1141 cgcttcatat acagacaacc tggaggaagt ttttgaaaat cacgaacaga tagaaatttc
     1201 gcgtttttac gaaaaactcc cgaaaccttc tctgatagcc ctgcaagact ggaaagaagg
     1261 ggaaatttat taccgcaatc cttccaaaga aaaagagcag ttttaacaga acgatatgct
     1321 tcagagaatt caaaccctgt ttctactgct tgcgtcagtt tgtatgctgg tggctacggt
     1381 aacccctctg gcttcttttt tctataacgg tgatcctgtt gtttttgaag cgatgggaat
     1441 gtacaggagc ggagaccttg aaagttccac ttgggcactt tttgctattg gtgccatcag
     1501 cgctcttttg ccgattgtga cagttttcct gtacaaaacc cgaattttac aaatccggat
     1561 atctattttt aatatctttc tgatggtcgg attttacctt tattttgggt ttttgatttt
     1621 caatatcaat cccgaagccg gattacagtt tcaaaagatc ggtattggtg caattatgcc
     1681 ggttattgcc attatcttga cctatctcgc catccgtagg atcggtgccg acgagatttt
     1741 agtcagatca ttccatcggc tgcggaaata acttttattt taacttgttt attcttcgag
     1801 gccggatcat attttccggc ttcagtattt catccaactc ctcttgggca atcagtttgt
     1861 tttccagtac gatatcataa acgccctttc ctgttcttaa tgcttcggca gctacccggg
     1921 tagagtcttc atatcccagg tacggattta atgccgtaac caagccaata ctatttaaca
     1981 ccatatccct gcagcgatct tggtttgccg tgattccgga aatacatttg cttctcagtg
     2041 tattgatgcc gttgataagc atttcgatgt tctccatcag cgaataggcg attatgggtt
     2101 ccataacatt cagctggagt tgtccggctt ccgatgccat ggtcacggtt aaatcattgc
     2161 caatgacctt gtaggcaatt tgattgacca cttcgggaat aaccggattt actttgccgg
     2221 gcataatgga agaaccaggc tgcatggggg gtaagttaat ctcgttcagt cctgttcttg
     2281 gtccagagga aagtaatcgc aggtcgttgc agatttttga tagttttacg gctaaccgtt
     2341 taataaccga tgagttcatg atgaaaaccc ccgtgtcgct ggtggcttcg atcaggttat
     2401 cggcagcaac gacggcaata tccgatattt ctgccaggta acgggtgcag attaccgtgt
     2461 attccgggtc ggagttgatt cctgtcccaa tagccgttgc tcccatattg gtttcgagaa
     2521 gtaactggcg gttttggctc aatcgtgata cttcttcctc taaagttgtg gcaaatgcat
     2581 caaactcttg cccaagtgtc ataggaacgg cgtcttgtaa ctgcgtgcgt cccattttta
     2641 tgacttctgc aaactctttc cctttttccc ggaaaacgac aatcaatttc tccaatgcgt
     2701 taatcaattc gccgatactt cggtataagg ctatttttag cgctgtggga taagcatcgt
     2761 tggtagattg agaaagattt acgtggttgt tggggtgaca atgggtgtaa tctcccttct
     2821 cgaaccctag aatttccaat gcccggttgg caattacttc gttggcattc atgttggttg
     2881 aggtgcctgc accaccttgt accatatcca ccacaaactg atcgtggagt ttgccgtgtt
     2941 taatttccct gcaggcttcc acaatggcat cggcaatggc ggggtttaac aattcaagtt
     3001 ctttgttggc cagtgctgca gctgctttca catctgccaa accccggatg agctgggggt
     3061 aatgatgaag ttttgaacgg ctaatgtcaa agttttctac tgcccggagg gtttgaatac
     3121 cgaaataact tgctgtgggg atttccatag ctccgataag atcgtgctcg gtgcgtgtgt
     3181 ctctgctaaa cttagaggtt gtcttgttca taataaatcg aatatttaaa gtaatccaag
     3241 gtgaatggtc tattggtttt ttcacattgg ccaaagttag ccattatttt gtattaaagc
     3301 tactttatcg atctttaaaa catgatttcc cctcgctggt gacagtccca aaaaaaggga
     3361 tcgcatagcg accccttaac taaatttgtc attgaggtat tatccggact aaaacgtcca
     3421 ctctttcgaa gtttttgctt cttccgtttt tgcgggaaca ggagcctggg tttttatggg
     3481 ctcactcgtc gtgttctgaa cgccggttcc tttatagtac tgctgtacgt tgggcataat
     3541 ttgctgaatg cgtgcgatgc gcttttcgtc cgtcgggtga gtgctaagaa attcgggtgg
     3601 agcaccacct tgagaacttt gtgccattct cgtccagaag ggaactgctg cctgaggatt
     3661 gtatcctgcc agggccatga caatgagccc tatttcatcg gcttcgtatt cttgtttacg
     3721 ggcatacggc atcatcactc caaattgagc gccaagaccg aatacctgtt gaccgatttg
     3781 tgcccctacc gaattgccta aaaggccgcc ggcaacagct cccccgtatt gcagggccat
     3841 ctgctggctg attctttcct gagcatgccg ggcaatgaca tgggcaactt cgtgtccgac
     3901 caccactgct aaagcttcct ccgtttgtgt caccggaagt aatccatcat agataactat
     3961 ttttccaccg ggcatggcaa atgcattcac gcttttgtct ttaaccaagt taaactccca
     4021 ctgataatac tgcagctcag attcataacc gttattcttg taaaatgttt ccacggcgtt
     4081 ggcaatccgg gagcccaccc tttttaccat ctcggcgttg gtcgtactcc tttcaagagg
     4141 cgcagaacgc ataaaatcct ggtactgctg caagctaagt gcaaccacct gttcgttcga
     4201 aaccaggctt aattgtcttc ttccagtgaa gggaacgctt ccacagcctt gtaaaagagc
     4261 gagggcgatg accattacta aagaaaattt caccggttga tttaattttt ttctttccat
     4321 agtttttgtt ttgttgttaa tttttgagtt ttactttagc cgtatattaa tcattaatgt
     4381 atgcgagact aaaacaacta gtatttcctt ttactaataa cacatttttg gggaagtagt
     4441 ttacccggca tcaaaaaaac acacatccac tgtaattttt ccgagaattg tccacatgac
     4501 ggaaaataga aatgcccctg tctttcgttt gtaaatcacg caaaaaaaag gtaactttga
     4561 atgttttttc aacctgaaac agtatatgtc attaaaagat agcaacgaag aagattttga
     4621 agataaagat atgcaaatga atccggaaga ggacgatatt cttctttccg attcttcgca
     4681 acactccaat ccccgaatta ttacccggct agccgaagac tcccccatga acctttcccg
     4741 gatgtatcag gactggtttc tggactatgc gtcgtatgtg attctggagc gtgcggttcc
     4801 gcatgttact gacggactga agccggtaca gcgccgtatt cttcacgcta tgaagcgaat
     4861 ggatgacggg aggtacaaca aggtggcgaa cattatcggt tttaccatgc agtttcaccc
     4921 gcacggtgat gcctccatcg gcgatgcact ggttcagctt ggccagaaag acatgctaat
     4981 cgactgtcag ggtaactggg gaaacgtaca taccggagat ggagctgctg cacccaggta
     5041 tatagaagca cgcttgacaa aatttgctct cgaggttctt tttaatgcca aaactaccga
     5101 atggaaaccg tcgtacgacg ggcgaaacaa agagcccgta acactaccgg ctaagttccc
     5161 gctgctgctg gcgcaaggca cggagggtat tgctgtgggc ctgacctcta aaattttacc
     5221 gcacaacttc aacgaactgt gcgatgcatc tgttgcatac ctgcagggga agaagttcag
     5281 cctgtttccc gacttccaaa ccggtggata tatcgatgtg gagaaataca acgatggcga
     5341 acggggcggg tcggtaaaag tacgtgcctc gataaaaaaa ctcgataaca agacattggt
     5401 tatcactagc ctgccgtatg gaaaaacaac ggcttctgtt gtagaatcga tcatcaaagc
     5461 aaacgaccgg ggaaagataa agatccgaaa agtggacgat attacggctg caaatgtgga
     5521 gattcatgtg cagcttgctc ccggggtttc gtccgacagg accatcgacg ccctatacgc
     5581 atttacggat tgtgagatca atatttcacc caattgctgt atcattgagg acgacaaacc
     5641 gcattttctt accgtcagcg atgtgctccg catctccacc gatcgcacta aagagcttct
     5701 ccgtttggaa ctggaaattg aaaaacagga aaaacaggag gccctccttt ttgcatcact
     5761 cgagaaaata tttatcgaag agcgcattta caaggacaat gagtttgaga atgcccggaa
     5821 tatggagatt gctgtcgccc atatcgataa gcgcctggaa ccgtacaagc cgtcatttat
     5881 ccgcgaaata acatcggagg atatcctgag gctgatggag ataaagatgg cgcgtatcct
     5941 gaaattcaat tccgacaagg ccgaagagaa tattgccagg ttcaggggcg agatagaaga
     6001 gatcaaccac catttggata acctgaccga ttacaccatc tcgtggtacc tgatgctgaa
     6061 agaaaagtac ggcaagaatt atccgcgacg tacggaaatt cgcagttttg agaatatcga
     6121 agctgttaag gttgccgaag ccaacgaaaa actatacatc aaccgtgaag aaggatttat
     6181 tgggaccggg ttgaagaaag acgaatttgt ctgcaactgt tccgatattg acgacatcat
     6241 cgttttcttc aaggacggaa aatacaaagt ggtaaaggtt agcgataaaa tgtttgtggg
     6301 aaaaggaatt ttatatgcta acgtctttaa gaagaacgat aagagaacca tttataacgt
     6361 gatttaccgt cacgggaaat cgggtgcaca ttacatcaag cgtttcccgg taacaggagt
     6421 tacacgagac aaggattatg atgtgacgca gggaaagact gcttcacgca tcgtttattt
     6481 cagcgcaaac cccaacggcg aagctgagac cgtgcgggtc atcctgaaac ccaaaccccg
     6541 tcaacggatt ttacagtttg agaaggattt cagcgagatc gccatcaagg gtaggggagc
     6601 gatgggaaat atccttacaa aagccgatgt gcaccgcatc atgctcaagc agaagggact
     6661 ttcaacattg ggtgggcgta aggtctggtt cga
//
LOCUS       DAOSDR010000160         6692 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrimonas sp. isolate AMR_MDS_1826
            NODE_4160_length_6692_cov_3.925117, whole genome shotgun sequence.
ACCESSION   DAOSDR010000160 DAOSDR010000000
VERSION     DAOSDR010000160.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863355
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrimonas sp. (wastewater metagenome)
  ORGANISM  Petrimonas sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Dysgonomonadaceae; Petrimonas.
REFERENCE   1  (bases 1 to 6692)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6692)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:10:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,720
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 35
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6692
                     /organism="Petrimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4160_length_6692_cov_3.925117"
                     /isolate="AMR_MDS_1826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2023866"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1625)
                     /locus_tag="PLI30_07405"
     CDS             complement(<1..1625)
                     /locus_tag="PLI30_07405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005641833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HOI79414.1"
                     /translation="MEHLLSQLNSKQREAVEFCDGPSLIIAGAGSGKTRVLTYKIAYL
                     LEQGLPPYYILALTFTNKAAREMKSRIADLVGEKKARQLWMGTFHAIFSRILRNEAER
                     IGFTPNFTIFDSSDSTNLVRLIIKEMQLDDKVYRPGAVHNQISRAKNGLITPGMYAQN
                     REIIEYDRASKRPQLFEIYQRYQNRLKAANSMDFDDLLMYTNILLRDNPDVLEDYRNR
                     FQFVLVDEYQDTNFAQHLIVKQLAELHHRVCVVGDDAQSIYSFRGANIDNILKFKSTF
                     PQTQIFKLEQNYRSTQNIVNAANSLIKKNKEQIFKEVFSENEEGEKIEVVSAFSDFEE
                     GLIVANKIARMRYELHTGFSDVAILYRTNAQSRIFEESLRKKNIPYVIYGGLSFYQRK
                     EIKDIISYFRLIVNPGDEEAFRRIVNYPTRGIGDVTVGKISESARLHNVSLWQILSSP
                     LQYNLDVNRGTARKLALFHELINGFIAGNETQNAYELAQTILKNTGIVKEAYEDLTPD
                     GMSRAQNIQELLNAINEFVSERQEQGEEDVRLV"
     gene            complement(1727..2029)
                     /locus_tag="PLI30_07410"
     CDS             complement(1727..2029)
                     /locus_tag="PLI30_07410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005797912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HOI79415.1"
                     /translation="MKGKKIELYDISAELEKEFGEPGSPERRQAEQEAWEDYNAQILM
                     DARKSARLTQEQLAERVGVDKGYISRVERGLIVPTIGTFYKIVAAMGLSVELRPHA"
     gene            complement(2010..2297)
                     /locus_tag="PLI30_07415"
     CDS             complement(2010..2297)
                     /locus_tag="PLI30_07415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004307571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HOI79416.1"
                     /translation="MKTLSSNERRKILKALLLFSEEEKIPYHYIKYIRDSIFEFRVSY
                     GNSEFRIFFIYDGNTIVVLLNCFKKKTQKTPQNEIEKAIRLKNEYYERKED"
     gene            complement(2443..2913)
                     /locus_tag="PLI30_07420"
     CDS             complement(2443..2913)
                     /locus_tag="PLI30_07420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019539110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI79417.1"
                     /translation="MKLRNLFIVTVLAVTAITTTANAQNYKTAFGARLGYDSGITLKH
                     FFAPASAFEGILSASPRYFQLTGLYEYQQPLPGAPGLDWYVGLGAHLGNVYYKDYNGG
                     RFLLGGDLIAGLEYAFPTAPFAISLDWKPSFNFTNDYNDYWFAGLALSLRYTFR"
     gene            complement(2982..3584)
                     /locus_tag="PLI30_07425"
     CDS             complement(2982..3584)
                     /locus_tag="PLI30_07425"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004299175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004797 - thymidine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HOI79418.1"
                     /translation="MYNENNPNETQRKGRIEVICGSMFSGKTEELLRRLRRAKIARQK
                     VEIFKPVIDVRYSQEEVVSHDKNSILSTPVEHSSNILLLASEVEVVGVDEAQFFDKGL
                     TDVCQQLADQGIRVIVAGLDMDFKRIPFGPMPALCAIADDVTKVHAICVRCGSLASYS
                     HRIVAGEKQVMLGEMHEYQPLCRKCYLQEQLFSTPPVNKF"
     gene            complement(3602..6190)
                     /gene="mutS"
                     /locus_tag="PLI30_07430"
     CDS             complement(3602..6190)
                     /gene="mutS"
                     /locus_tag="PLI30_07430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009318392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HOI79419.1"
                     /translation="MMKQYNEIKQQHPDAVLLFRVGDFYETFSDDAITASEILGITLT
                     RRANGAAQFVELAGFPHHALDTYLPKLVRAGKRVAICDQLEDPKLTKTIVKRGITELV
                     TPGVSINDNVLNHKENNFLAAVYSANGKTFGISFLDISTGEFLTTEGNKDETDKLLSS
                     FSPKEILIERGSKRKLGEYFGADYFFFELDDWIFTDDAARERLLNHFNTKNLKGFGVQ
                     HLPLGIIASGAVLHYLDITQHTQISHITSLRQLDESRYVRLDKFTVRSLELISTINEG
                     GKSLLDILDKTISPMGSRMLRRWMVFPLKDVRPIEERLDVVENFFRETALKELLEEKL
                     SLIGDLERIISKAAVGRISPREVVQLKVALTAIEPVREAFLSSENAGLQEMGRKLDPC
                     FVIRDRIEREIFPDPPALLNKGGVIKKGVNGQLDELREIAYSGKDYLLQLQQRKSTET
                     GIPSLKIAFNNVFGYYIEVRNTHKDKVPETWIRKQTLVSAERYITEELKEYEEKILGA
                     EEKIVSLETQLYNALVESLIEYIPTIQGNATVMARADCLLSFAKIARENKYIRPEINE
                     SDAIDIKSGRHPVIEKQLPHDEPYIANDVYLDRDSQQIIIITGPNMAGKSALLRQTAL
                     ITIMAQIGSFVPAESAKIGLVDKIFTRVGASDNISLGESTFMVEMNEAANIINNISER
                     SLVLFDELGRGTSTYDGISIAWSIVEYIHEHPRAKAKTLFATHYHELNEMEKSFKRIK
                     NYNVAVKEIDNKVIFLRKLVPGGSEHSFGIHVAKMAGMPQSITKRSEAILEQMETANR
                     RQGIKKPIKDIVEKREGYQLSFFQLDDPVLSQVRDEILNLDVNNLTPIEALNKLNEIK
                     KIVKGK"
     gene            complement(6456..>6692)
                     /locus_tag="PLI30_07435"
     CDS             complement(6456..>6692)
                     /locus_tag="PLI30_07435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009318150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI79420.1"
                     /translation="EAAELAGKADIFVIIGTSMNVYPAAGLLDYVKKNVPVYLIDPKD
                     VNTGRYDVFHIKKGASEGVKELRQRLGDPTIGRV"
BASE COUNT         1723 a         1618 c         1366 g         1985 t
ORIGIN      
        1 tccaccaacc gaacgtcttc ctcaccctgt tcctgccttt cagacacaaa ttcgtttatg
       61 gcattcagca attcctgaat attttgtgcc cggctcatcc cgtctggggt caggtcttcg
      121 taagcctcct tgacgatacc cgtatttttc agtatcgttt gagccagttc ataagcgttc
      181 tgcgtttcgt taccggcaat aaatccgttt atcagttcgt gaaacagcgc aagtttccgg
      241 gctgtgcccc tattcacgtc caggttatat tgcagcggcg acgagagtat ctgccacaag
      301 ctgacgttat gcagacgggc gctttcactt attttaccta ccgtaacatc gccaattccg
      361 cgtgtggggt agttcactat gcggcgaaac gcctcttcat ctccgggatt tacaataagc
      421 cggaaatagc tgatgatgtc ttttatctcc tttcgttgat aaaaagaaag ccccccataa
      481 ataacatacg gaatattttt cttgcgcagc gactcttcaa agatacgcga ctgggcattg
      541 gtacgataaa ggatggcaac atccgaaaat ccggtatgca attcgtagcg catccgtgcg
      601 attttattgg ctacgataag cccctcctca aaatcggaaa aggcactcac cacttcaatt
      661 ttctcaccct cttcgttttc cgagaaaacc tctttgaaga tctgctcctt attttttttt
      721 ataagactgt tggcagcgtt tacaatgttt tgcgtggacc gataattttg ttctagctta
      781 aaaatttgcg tctgggggaa agtggattta aatttcagga tattgtcgat attggctccg
      841 cgaaacgaat aaatgctttg cgcatcatca cccaccacac aaacccggtg atgcagttct
      901 gccaactgct ttacgataag gtgttgcgcg aaattggtat cctgatactc gtcaacgagc
      961 acaaactgaa aacggttccg gtaatcttcc agcacatcgg ggttatcgcg cagcaggata
     1021 ttggtataca tcaacagatc atcaaagtcc atgctgttgg cggccttcag ccggttttgg
     1081 taacgctggt aaatttcgaa cagttgaggg cgtttggaag cacggtcgta ctcaattatc
     1141 tccctgttct gggcatacat tccaggcgtt atcagaccgt tcttcgctct cgatatctgg
     1201 ttatgaacag ctcccggcct gtacaccttg tcgtccagct gcatctcttt aatgataagg
     1261 cgtaccagat tggtggaatc ggaagaatcg aaaattgtaa aattaggagt gaacccaatc
     1321 cgttcagcct cgttccgcaa tatacgtgaa aagatggcat gaaacgttcc catccacaac
     1381 tgacgtgctt tcttttcccc caccaagtcg gcaatccgcg atttcatttc ccgagcagcc
     1441 ttgttggtaa aagtcagcgc cagtatataa tatggcggaa ggccctgttc cagcaggtaa
     1501 gcaattttat aggtcaggac acgggttttt cccgatccgg caccggcaat aatcagcgac
     1561 gggccatcac agaactcaac ggcttcgcgt tgtttggaat ttaattgact gaggagatgc
     1621 tccatttttt cgaataatat agtacaaaaa tacgattttt aagcgtaccg tcataaaaaa
     1681 accgctctcg gttgagaacg gtttttataa ttgttcatac tctctcttaa gcgtgtggac
     1741 gtaattcaac cgaaagtccc atagcggcta caattttata gaaagtgccg atggttggca
     1801 caatcaaccc gcgctcaacc cgtgaaatgt accctttatc taccccaaca cgttctgcca
     1861 attgttcctg ggtaagacga gcactttttc gagcatccat caatatctgt gcgttataat
     1921 cttcccatgc ttcctgttct gcttgccgcc tttcaggtga accgggttcg ccaaactctt
     1981 tttccaattc agcgctgata tcgtaaagtt caatcttctt tcctttcata atattcattt
     2041 tttaatctga tggctttttc tatttcattc tgtggtgttt tttgcgtttt cttcttaaaa
     2101 caattgagta atacaacaat tgtatttcca tcgtaaataa agaaaattct aaattcacta
     2161 ttcccataac tcactctaaa ttcgaaaatt gaatctcgaa tatatttaat ataatgatac
     2221 gggatttttt cttcttcact aaatagcagc agggccttta atatttttct gcgttcatta
     2281 ctacttaatg ttttcatgaa cgattcataa tatcctccgt aagctactat ttttcgtccc
     2341 ataagacaaa agtaacaaaa gttatatcat aatacaactt ttatggtaaa aaaaaccgct
     2401 ctcggttgag aacggttttt ataaagtacc ggaatctggt tcttatctga acgtgtagcg
     2461 taaactaagt gctaacccgg caaaccagta gtcgttgtag tcgttggtaa agttaaacga
     2521 gggtttccag tccagtgaaa tcgcaaaggg agccgttgga aacgcgtact caagccctgc
     2581 aattaaatcc cctcctagca gaaaacgacc gccgttataa tccttgtaat agacatttcc
     2641 aagatgagct cccaaaccca cataccagtc caggccgggt gctcccggta gcggttgctg
     2701 gtattcatac aacccggtca attgaaaata acgcggagaa gcgctcaata taccttcgaa
     2761 tgcgctagcc ggcgcaaaga aatgcttcag cgtaataccg ctgtcataac ctaagcgggc
     2821 accaaaagcg gttttgtagt tctgagcatt tgcagttgtg gttatggctg taacagccaa
     2881 gactgtaacg ataaataagt ttctaagttt cataagttaa tgttttaata aatatttact
     2941 caccgtgtat ttaacacttt tgtctatata aacacccctg tttaaaattt gtttaccggt
     3001 ggagtagaaa ataactgttc ctgcaaataa catttcctgc aaagcggctg atattcatgc
     3061 atttccccca acattacctg tttctcacca gccactatac ggtgtgaata gctggccagt
     3121 gaaccgcaac ggacacagat ggcatgaact ttggtcacat catcggcaat ggcacaaagg
     3181 gcgggcatcg gcccgaacgg tatccgttta aaatccatgt cgagtccagc cactattacc
     3241 cggatacctt ggtcggcaag ctgctggcaa acatcggtaa gccctttgtc aaaaaactga
     3301 gcttcgtcta ctcccaccac ttctacttca gacgcaagca gcaggatgtt actggaatgc
     3361 tcaacgggtg ttgaaagaat agagtttttg tcgtgcgaca ctacctcctc ctgcgaataa
     3421 cgcacgtcga ttaccggttt aaatatttcc actttctggc gggcaatttt cgcccggcga
     3481 agcctgcgaa gcagttcttc ggtctttccg gagaacatgg aaccgcaaat gacttcgata
     3541 cgccctttgc gctgggtttc gttgggattg ttttcgttgt acatactgag cggagagcga
     3601 attatttccc tttcactatt ttcttaattt cattcaattt attcaaagcc tcaatagggg
     3661 taaggttatt gacatccaga ttgagtattt cgtcacgaac ctggcttaaa acagggtcgt
     3721 ccagctggaa aaaactcaac tgatagccct cccgtttttc aacaatatcc tttatcggct
     3781 ttttgatgcc ctgtctgcga ttggctgttt ccatctgttc caggatcgct tccgaccgtt
     3841 tggtaatgct ctgcggcatc ccggccattt tggcaacgtg aataccaaaa ctgtgttcac
     3901 tccctcccgg cacgagtttt cgcaagaaaa tgactttgtt gtcaatctcc tttacagcaa
     3961 cattgtagtt tttgattcgc ttaaacgatt tttccatctc attcaactcg tggtagtggg
     4021 tggcaaaaag agtcttggct tttgcccggg ggtgctcatg aatatattcc acgatcgacc
     4081 acgcaataga aatcccatcg taggtacttg tacctctgcc caattcatca aaaagcacaa
     4141 gactccgttc cgagatatta ttgatgatat tggctgcttc gttcatttcc accataaacg
     4201 tggattcgcc cagcgatata ttgtccgatg cgccgacgcg ggtaaagatc ttatccacca
     4261 gtcctatctt tgcggattct gccgggacaa aactccctat ttgcgccata atagtgatca
     4321 gcgccgtttg gcgcagtaag gccgatttcc cggccatgtt gggaccggta ataataatga
     4381 tttgctgtga atccctgtcg agatacacat cgttggcaat atagggctca tcatgcggaa
     4441 gctgtttttc gatcacagga tggcggccac tcttgatatc gatggcatcc gactcgttta
     4501 tttcgggacg gatatacttg ttctcccgag ctatttttgc aaaagacaac aggcaatctg
     4561 cccttgccat aacggttgcg ttaccctgta tagttggaat atactctatc aggctctcca
     4621 caagagcgtt gtatagctgt gtttccagcg atacaatttt ttcttcggca cccaatattt
     4681 tttcttcata ttctttcagt tcctcggtaa tgtaacgttc tgcgcttacc agcgtttgtt
     4741 tccgtatcca cgtttcaggg accttgtctt tgtgcgtatt ccgcacttca atgtaatatc
     4801 cgaaaacatt attgaaagca atcttcagag agggtatacc cgtttccgta cttttacgtt
     4861 gctgcaactg taggaggtaa tctttgcccg agtaggcaat ttcgcgaagc tcatccaact
     4921 gtccgttcac tcctttttta atcacccccc ccttattcag cagagccggc ggatccggaa
     4981 agatctcccg ttcaatccga tcccggataa cgaagcaagg atctaatttc ctacccatct
     5041 cctgaaggcc ggcattttcg gacgacagga aggcttcacg taccggttct attgctgtta
     5101 aagccacctt gagctgtacc acttcgcggg gagagatcct accgacagcc gctttagata
     5161 tgattcgttc caagtcaccg ataagcgaaa gtttttcctc gagcagctct ttcaatgctg
     5221 tttcgcggaa aaagttttcc accacatcca ggcgttcttc tatgggcctg acatccttga
     5281 ggggaaacac catccagcgg cgtaacatgc gcgaccccat gggtgagatg gtcttatcga
     5341 gtatatcgag taaacttttt ccaccttcat ttatcgtgga aatcagctcc aggctgcgta
     5401 ccgtgaattt atccagtcgc acgtaacggc tttcatccag ttgcctgagt gaagtaatgt
     5461 ggctgatctg agtgtgctgt gttatatcca gataatgcaa cacagctccg gacgcaataa
     5521 ttccaagggg caggtgctgt accccaaacc cttttaggtt ctttgtgttg aaatggttta
     5581 ataagcgttc gcgggctgca tcatcggtaa atatccaatc gtcgagttcg aaaaagaagt
     5641 agtccgcccc gaagtattct ccaagcttac gcttactgcc ccgctcgata aggatctcct
     5701 tgggtgaaaa gctggaaagc aatttgtctg tttcgtcctt attaccttcg gtagtgagaa
     5761 actcacccgt tgagatatcg agaaaagaga tgccgaacgt ctttccgttt gccgaataaa
     5821 cggctgccag aaaattattc tccttgtgat tcaacacatt atcattaata gatacacccg
     5881 gcgtaactaa ttcggtgata ccgcgcttaa caatcgtttt ggtaagcttc gggtcttcca
     5941 gctgatcgca gatagccaca cgtttccccg cacgaaccag tttaggcaaa tacgtatcaa
     6001 gagcgtgatg cggaaatccg gccaattcca cgaattgtgc tgcaccgttt gctcggcgcg
     6061 tcagcgttat tcccaggatc tccgaagccg tgatcgcatc atccgaaaaa gtctcgtaaa
     6121 aatcacctac cctgaagagt aaaacagcat cgggatgttg ttgtttaatc tcgttgtact
     6181 gcttcatcat cggggtttca actacctgct tactcactaa tctctataaa ttaaattatc
     6241 caataaatta ttactgtgtt ttagattgaa tgagattgat ggcggattga acaagattga
     6301 atatgggcgg cagtttatca atcttgttca atcttaatta atctttctca atcttgacaa
     6361 atacgacatt attttggatc atccctttat taaataatgt gtaatgattt ttttctgaaa
     6421 aaggataaca aagatactat ttttttattt acgatttaca ctcgccctat cgtcgggtcg
     6481 cccaatctct ggcgcaactc cttcactccc tccgatgccc ctttttttat atgaaataca
     6541 tcgtaccggc cggtattcac atctttggga tcaatcaggt aaacgggaac attttttttc
     6601 acatagtcca gtaatcccgc agccggatac acattcattg aagtgcctat tatcacaaaa
     6661 atatctgctt tcccggccaa ttccgcagcc tc
//
LOCUS       DAOSDR010000161         6665 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrimonas sp. isolate AMR_MDS_1826
            NODE_4176_length_6665_cov_4.654009, whole genome shotgun sequence.
ACCESSION   DAOSDR010000161 DAOSDR010000000
VERSION     DAOSDR010000161.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863355
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrimonas sp. (wastewater metagenome)
  ORGANISM  Petrimonas sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Dysgonomonadaceae; Petrimonas.
REFERENCE   1  (bases 1 to 6665)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6665)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:10:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,720
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 35
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6665
                     /organism="Petrimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4176_length_6665_cov_4.654009"
                     /isolate="AMR_MDS_1826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2023866"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            275..>1863
                     /locus_tag="PLI30_07440"
     CDS             275..>1863
                     /locus_tag="PLI30_07440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI79421.1"
                     /translation="MRNFYVILRTARLALTALVTGILLLASTAGFGQVATVTTDKTDY
                     IPGEIVFIEGSGWLPGETVLLVIDHLIYTSYPSDTLSAVANATGNIYNNEYLITQAYL
                     GEAFLLTAIGQTSNLTASTNFLDSGGDFTDVDFTAAAPYTYNHEIGGGAFNDRHVGKY
                     DDIVESLEAGDFECGDIVTYLMHLKIADAPDNANQTIELDFSFLATTTGQEGVALADV
                     VYAGINYGSVVNGAGPGGVDSGVNDDGGSTAVITHEVLTGPLYTAGSELRATIKIDDL
                     EAGEEVVLRLDVLLSCKPGSRPTGNLQAKFDQGRIIWTAEDGTLPVPDIIPGGEQTIP
                     FKQFGQIPPPLDCGINGNEGCGGSTNTYTAADQSIPDATLTYAWTIAGDGEFVGSTTG
                     KSVSVLAYGSGSYTLGVKIMSATRGYIECSSTYDIFPHPTVTVNSPVICKSDGSATLT
                     ATPGTAGTYNYDWTVPEGATDPGNVASFSATVAGTYSVVITDVNNCDGSGTGSLTIEP
                     EPVLSNASLTVCENVPGGVLDG"
     assembly_gap    1864..1963
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1964..4587
                     /locus_tag="PLI30_07445"
     CDS             <1964..4587
                     /locus_tag="PLI30_07445"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOI79422.1"
                     /translation="YFTGYTLNCDASAKITLKVEPRPVLSNASLTVCENVPGGVLDGT
                     FDLTQAATGVNPGTMNIVYSDAGGPIASPSAYNAVNGSQVTVTAYFTGYTLNCDASAK
                     ITLKVEPRPVLTPATDWFCKDDVPGAFDPNNYNDAILDAPQNVGDFTFEWSGGPLSLP
                     VDIYTAAVTVFNVTVTNKATLCSSISTLTITVYPHPVIQSLVVSKPLSWIGAHDAEID
                     LTVAGGTTPYVYKWTGSSGGVVPAGQENKQDLTGLVAGTYTILVTDAHGCTATSFITI
                     QPPGGYDIKLVPDPILCAYLVDGKLVYTSEGAVTVSFIGDEGVLSPYRTFAWTGPGGF
                     TASTQNIGNLDVPGIYSLTIKFWNDVAKTDLALTLTGYSAEVLAPSDGEALSVSASAK
                     TNCDEGKAGLPGGTVTVKGTGYERLRLYRASDDALLEDVGTSTSPYTFLSKLFSGDYY
                     VVASAPNTLGKPDACEVSAEVKVDKCYWFEILKLTQGIVNPLKDWRFSIYKGPDGFGT
                     GALAADGTLGDLDGILDFGFVNLDPAKTYTICEENMAAGWSALWLTEIGGNETILMTY
                     NPNADDVPPQDLGNRCFDIGAGTAYPLYPDDVFRIKVDNTYPGGTARTPGYWKNWNTC
                     TGGGQAKTALANGGWQAGYWLLNDVLNNPGITLGSLVLKSCTDAVNILDMRDLKSTKK
                     MSSDAAYTLACHLTAYRCNEAAGAYGCSYAGKTAAAADALLIKIGFNGTGSYLRSKDK
                     NYNYALQLAAILDKYNNNRFTETDCLNNPPLPEKSAEIAIVVPELLEASELKVYPNPF
                     SDKVTFEFVSGVDAYGVLDLYNIAGQRVARILDRYVVKGVLNKIEYEPVDVISGVYLY
                     KLRLDDQVQVGRLVYKK"
     gene            5027..>6665
                     /locus_tag="PLI30_07450"
     CDS             5027..>6665
                     /locus_tag="PLI30_07450"
                     /inference="COORDINATES: protein motif:HMM:NF012931.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HOI79423.1"
                     /translation="MKKIFSATLFCLFIFHLAAQKVDVIPLPAEMTEKEGSFLISPET
                     RILFDASNKELSEVVELSSHLLAKALSFEPVVAEPGSTNPKGNILVQLNSPFDPDIKT
                     EGYKLSIQNDRIFLNANTTAGIFYGFQTLWQMLTAEPGNRLSCVEITDYPRFAWRGLH
                     LDVSRHFMPVEFIYKYIDYIALHKMNVFHWHLVDDQGWRIEIKKYPKLTEVGAWRVDR
                     EDRHWDSRPLAQKGEKATYGGFYTQQQVKDIVAYAQKRQVIIVPEIEMPAHVMSALAA
                     YPEYSCNGKNLGVAPGGVWPITQIYCAGNDATFGFLEEILSEVMELFPSTFIHIGGDE
                     ADKTEWKTCPKCQQRMKAEGLKDEHELQSYFIERIEKFLNSQGRRLIGWDEILEGGLA
                     PQATVMSWRGEQGGIEAAKMGHNVVMTPGSHCYFDHYQGDPSLEPLAIGGYTTLKKVY
                     QYEPVPAELNENEARFVLGAQANVWTEYMPDGKQVEYMIFPRLSALAEVVWSPKQSRD
                     WPGFSRRMETQYQRYKRVGANFSTSAYQVTSSAVPDME"
BASE COUNT         1669 a         1530 c         1741 g         1625 t
ORIGIN      
        1 tgtcattttt caggaaagaa actacccgtt tttacaaaaa taaacccccc gtcacgacca
       61 gacggtgtat cataattcag taaaaaaacc gattgactgc cttccaaagt tgccgtaact
      121 ttttttatca agataaggga acaggaccca ggctattgtg cttatgctcc gggagtaaat
      181 tcccgataac cattttggag gacagttttt tgcggctttt ggaagaaaaa gtgtgaggac
      241 atggaaacag tgttgaaacg ttaagaagaa gattatgaga aacttttacg taattctgcg
      301 aactgcccgt ttggcgctga cagcgctggt gacgggcatt ttactgttgg cgtcaacggc
      361 cggttttgga caagtagcta ctgtaactac tgataaaact gattatatcc ctggcgagat
      421 tgtatttata gaaggttctg gctggttgcc cggagaaaca gttcttttgg taatcgacca
      481 tcttatctat accagttatc caagtgatac gctctcggca gttgccaatg ccaccggtaa
      541 tatttataac aatgaatacc tgattaccca agcgtattta ggcgaagcct tcttgcttac
      601 ggccatcggc caaaccagca atttaaccgc ttccacaaat tttttagata gtggaggcga
      661 tttcacggat gtggatttca cagccgctgc accttatacc tataaccatg agataggtgg
      721 cggggcgttt aacgaccgtc atgtagggaa gtatgatgat attgtcgaat cgctggaagc
      781 cggcgatttt gagtgcggcg atattgttac ctatttaatg catcttaaaa ttgccgatgc
      841 tcctgataat gcaaaccaga ccattgaact ggatttctcc ttcctggcta caaccaccgg
      901 acaagaaggg gtcgccctgg ctgacgtggt ttatgccggt atcaattatg gttcggttgt
      961 taatggtgca ggccctggag gagtagactc cggtgtaaat gatgacggag ggagtactgc
     1021 cgttatcaca catgaggtct tgacgggacc cttgtacacg gcaggcagtg agttgcgcgc
     1081 aaccataaaa atcgatgatc tggaagccgg cgaagaggtt gtgttacggc ttgatgtatt
     1141 actttcctgt aaaccgggaa gccgcccgac aggaaacctg caggccaaat ttgaccaggg
     1201 aagaataatc tggactgctg aagatggaac acttcccgtg ccggatatta ttcccggcgg
     1261 cgaacagact atcccgttta aacaatttgg gcaaatcccg cccccgctgg attgtggaat
     1321 caacggaaat gaaggttgtg gaggaagtac aaatacttac accgcagcag atcagagtat
     1381 tccggatgct acgttaacct atgcctggac catcgcggga gacggggagt ttgtgggtag
     1441 tacaaccgga aaatcggtga gtgttcttgc ctatggctcc ggatcttata ccctgggtgt
     1501 taaaatcatg tctgcaacca gaggatacat tgagtgtagc agtacctatg acatttttcc
     1561 tcatccaacc gttacagtta acagtccggt tatctgtaaa tcggatggtt cagcgacact
     1621 tacggcaacg ccgggaactg ccgggactta caactacgac tggactgttc ccgaaggagc
     1681 taccgatcct ggtaatgttg ccagtttttc tgctaccgtt gccggaacct acagcgtggt
     1741 aatcaccgat gtcaacaatt gtgacggaag tggaactggt tcgctgacca ttgagcctga
     1801 accggtactg agcaatgcca gcctgacggt ttgcgaaaat gttccgggcg gagtgctgga
     1861 cggnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1921 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnncctactt caccggttac
     1981 acgttgaact gcgatgcgtc ggccaagatc acgctgaaag tagaaccgcg accggtattg
     2041 agcaatgcca gcctgacggt ttgcgaaaat gttccgggcg gagtgctgga cggcactttt
     2101 gatctgaccc aggcagctac cggcgtgaat ccgggcacca tgaacattgt gtacagcgat
     2161 gccggcggac cgattgccag cccgtcagct tacaatgcgg tgaatgggag ccaggtcact
     2221 gttacggcct acttcaccgg ttacacgttg aactgcgatg cgtcggccaa gatcacgctg
     2281 aaggtagaac cgcgaccggt attgactccg gctaccgatt ggttctgtaa agatgatgta
     2341 ccgggagctt ttgatccgaa taattacaac gatgccattt tggatgcccc gcagaatgtg
     2401 ggtgatttca cctttgaatg gagcggtggg ccactgagcc tcccggtcga catttatact
     2461 gctgctgtga ccgtattcaa tgtcaccgta accaataaag ctactttgtg tagcagcatc
     2521 tccacgttga ccattacggt ttatccgcat ccggttattc aaagcctcgt tgtaagcaaa
     2581 ccactgtcct ggattggcgc tcacgatgcc gaaatcgatc tgacggtggc tggaggaacg
     2641 acgccttatg tatacaaatg gaccggaagt agtgggggcg tagttcctgc cggacaggaa
     2701 aacaagcagg atttaaccgg tctcgtagcc ggaacataca ctattttagt taccgatgcc
     2761 cacgggtgta cggcaaccag tttcattacc attcagcccc cgggcggata tgacatcaaa
     2821 ctggttcctg atcccatctt gtgtgcttat ttggttgatg gcaagctggt ttataccagc
     2881 gagggggccg ttacggtttc tttcatcgga gatgaagggg tgctgagtcc gtacagaacc
     2941 tttgcctgga ccggacctgg cggttttact gcttcaacac aaaatatagg caatctggat
     3001 gttcccggta tttacagttt gaccattaaa ttctggaatg atgtagctaa aaccgatctc
     3061 gcattgacgc ttaccggtta ttcggccgaa gtacttgcac cttctgatgg agaagccttg
     3121 tcggtatcgg ccagcgctaa aaccaattgc gacgaaggta aagccggatt acccggcggt
     3181 actgtcacgg ttaaaggtac cggttacgaa aggttacgct tatacagggc cagcgacgat
     3241 gctttattgg aggatgtagg tacttctacc tcgccatata cctttttaag caagttgttc
     3301 tcaggtgact attatgtggt tgcttcggct cccaataccc tggggaagcc cgatgcctgt
     3361 gaggtaagcg ctgaggtgaa ggttgacaaa tgctactggt tcgaaatatt gaaactcacg
     3421 cagggaattg tcaatccgtt aaaagactgg agattctcca tatacaaagg accagatggt
     3481 tttggaacgg gtgcgcttgc cgctgatggt acattagggg atctggatgg catcctggat
     3541 ttcggatttg taaatctcga cccggcaaaa acctacacca tttgcgaaga gaacatggcc
     3601 gcaggctggt cggccttatg gctgacggag attggcggga atgaaactat tttaatgacc
     3661 tacaatccca atgcagacga tgttcctccg caggatttag gtaaccgctg tttcgatatt
     3721 ggcgctggaa ccgcttatcc gctttatccg gatgatgtat tccggattaa agtcgataac
     3781 acttacccgg gaggtacggc cagaactccg ggatactgga aaaactggaa tacctgcacc
     3841 ggtgggggcc aggccaaaac agccctcgcg aacggtggct ggcaggccgg ttattggtta
     3901 ctcaacgatg tattgaataa tccgggaatt acattaggct ctctggtctt aaagtcctgt
     3961 accgatgctg ttaatattct cgacatgcgg gatttgaaaa gcaccaagaa aatgtcaagc
     4021 gacgcagcgt atacactggc ctgccactta acggcctatc ggtgtaacga ggctgccgga
     4081 gcttatggtt gctcgtatgc cgggaaaacc gctgcagctg ccgatgcatt actgatcaag
     4141 attggcttta atggtaccgg aagttatctg cggtcgaaag acaaaaatta taactatgcc
     4201 ttgcagctgg ccgcaattct ggataaatac aataacaacc ggttcactga aaccgattgt
     4261 ttgaacaatc ctcctctgcc ggaaaaatca gctgaaatcg caattgttgt tccggaattg
     4321 ctggaagctt ccgaactgaa ggtttacccg aatccgttca gcgataaggt caccttcgaa
     4381 tttgtttccg gagtggatgc gtacggagta ctcgatcttt acaatatcgc cggtcaacgg
     4441 gttgcacgaa tcctggatcg ctatgttgtg aagggggttc tgaataaaat tgaatacgaa
     4501 cctgttgacg taatttccgg agtttatctc tacaaattgc ggctggacga tcaagtacag
     4561 gtaggcaggc ttgtttacaa gaaataactg agctaaaaaa aaggagtccc ttatttttaa
     4621 tcctgtaaag cgaaaagctt tcgacggaga aaagttcctc cgtattttcc ttgagaggct
     4681 gggttgaaag acgccagcct ctttctttcg gcaaaaattt tcggtgacgg aagccgggaa
     4741 cgggggcggt tttccttgca gcccgaataa cgtgaagagg gccgtgggtg ctggttttcc
     4801 tgcagtgtag ctgctggttt tcaggggtgt ggtcgaggcg attcgtttcg tcaccggcgg
     4861 gtcagtttat ccggtgtaac acttttttct gtcaaaattt ctaattttgt acgcgctgat
     4921 ttttagggtg gcaactcaaa agtagacgac cggaataggg agtgaccagc cgggcgttta
     4981 gatatgccga ccggaaaaca atgataaaaa actaaaattt tcaccaatga agaagatatt
     5041 ttcagcaacg cttttctgct tgtttatttt tcatctggca gcacaaaaag tggacgtgat
     5101 tccgcttccg gctgaaatga ccgaaaaaga aggcagtttt ttgatatccc ccgaaaccag
     5161 gatactcttc gatgcttcca ataaagagtt aagcgaagtc gtggagcttt ccagccacct
     5221 gctggccaaa gccctctcgt ttgagccggt tgtggcagaa ccgggaagca ccaatccgaa
     5281 aggaaatatc ctggtgcagt tgaattcccc cttcgatcct gatattaaaa ccgaagggta
     5341 caaactctcc attcagaacg atcgcatttt tttgaatgcc aacaccacgg ccggtatttt
     5401 ttacggtttc caaaccctgt ggcaaatgct cacggccgaa cccgggaacc ggttgtcgtg
     5461 cgtggaaatt accgactatc cgcggtttgc ctggcgggga ctgcacctcg atgtgtcgcg
     5521 ccattttatg cctgtcgagt tcatttacaa atacatcgat tacatcgccc tgcacaaaat
     5581 gaacgtgttt cactggcatc tggtcgatga ccagggatgg cgcattgaaa ttaaaaaata
     5641 cccgaaactg acggaagtcg gggcctggcg ggtcgatcgc gaagatcggc actgggacag
     5701 ccggccgctt gcccagaaag gcgaaaaggc cacctacgga ggtttttaca cccagcagca
     5761 ggttaaagac attgtagcct acgcccaaaa acggcaggtg atcattgtgc ccgaaattga
     5821 aatgccggcc cacgtgatgt cggccctggc tgcctatccc gaatattcgt gcaacggcaa
     5881 aaacctggga gtagcccccg gcggagtgtg gccgatcacc cagatttact gcgccggcaa
     5941 cgacgcgact ttcggctttc tggaagagat cctgtcggaa gtaatggagt tgttcccttc
     6001 cacatttatt catatcggtg gcgatgaggc cgacaaaacc gaatggaaaa cctgcccgaa
     6061 atgtcagcaa cggatgaagg cggaaggatt aaaggatgaa cacgaattgc aaagctattt
     6121 cattgagcgg atcgaaaaat ttttaaacag ccaggggcgg cggctgatcg gctgggacga
     6181 gattctggaa ggagggctgg caccccaggc tacggttatg tcgtggcggg gggaacaggg
     6241 cggaatcgag gctgccaaaa tggggcacaa cgtcgtgatg actcccggct cgcattgcta
     6301 ttttgaccac taccagggcg atccttcgct tgagccgctg gccattggcg gttataccac
     6361 cttgaaaaag gtgtaccagt acgaaccggt ccctgcggaa ctgaatgaaa acgaagcccg
     6421 ttttgtgctg ggcgcccagg ccaatgtgtg gacagagtac atgcccgacg gtaagcaggt
     6481 ggaatacatg attttccccc gtttgtcggc gctggccgaa gtggtgtggt cgccgaaaca
     6541 gagccgcgac tggcccggat tttcgcgccg gatggaaaca caataccagc ggtacaaaag
     6601 ggtgggggcc aacttttcaa ccagcgccta ccaggtgacc tcttcggccg ttcccgatat
     6661 ggaaa
//
LOCUS       DAOSDR010000162         6664 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrimonas sp. isolate AMR_MDS_1826
            NODE_4177_length_6664_cov_3.644727, whole genome shotgun sequence.
ACCESSION   DAOSDR010000162 DAOSDR010000000
VERSION     DAOSDR010000162.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863355
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrimonas sp. (wastewater metagenome)
  ORGANISM  Petrimonas sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Dysgonomonadaceae; Petrimonas.
REFERENCE   1  (bases 1 to 6664)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6664)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:10:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,720
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 35
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6664
                     /organism="Petrimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4177_length_6664_cov_3.644727"
                     /isolate="AMR_MDS_1826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2023866"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..720
                     /locus_tag="PLI30_07455"
     CDS             <1..720
                     /locus_tag="PLI30_07455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="SusC/RagA family protein"
                     /protein_id="HOI79424.1"
                     /translation="YVGRKIGEIWGYNTVGIYQSQEEADAGPDQTYFFSRWGEGDIEY
                     ADLNKDGKINPGNNTLDDHGDLSIIGNSTPRFSYGINLGLDWKNFDLSVFCQGVGKRD
                     AAPSGNMFYGITGDVWQSSVFVQHLDYWSSENPNAYYPKPYLGTNEHSKNIQTQTRFL
                     QNAAYFRLKSFQFGYTLPVEKKIKFPIEKIRFFITGENLFTLTKMNKTFDPETAFVGA
                     YGSGKAYPLRRTFSFGLNVDL"
     gene            741..2516
                     /locus_tag="PLI30_07460"
     CDS             741..2516
                     /locus_tag="PLI30_07460"
                     /inference="COORDINATES: protein motif:HMM:NF019592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HOI79425.1"
                     /translation="MKKIIYLIVFSFTVLTSCDLDDYLNKTPLDSITDVDYWKSASDL
                     QLYVNQFYTMLPQFPSWGGGYLWEDNNSDNMASRFYDNRLAGTNTITTNNGSWDYSRI
                     RSINIMLNKYKEMSVSESEIAPYVGEAYFFRAFAYFNLVRQYGDVPYVSQVLTPDSEE
                     LFAARTPRKEVIDYILDDLSQAIEKLPPKAASTRNRINKESALLYKSRVALYEGTWEK
                     YHQETVFGVKGSNGQEYLEIAASAAKQLMDMNTIELYSTGNPEVDYASLFNRDDLSGI
                     GEVLLTRDYNISLNTVHNLQRYTTSGAGTGLTKSLIESYLCEDGKPISLSPLYRGDKS
                     LEMVVENRDPRLRQMIYWKEGEPRRTQNGVLVPGYEFTRPYIDQGGEGGNTTGYMLKK
                     GLDTESKGQPEQNASETSMPIFRYAEVLLNYAEAKAELGTITQNDIDLTINKIRSRAG
                     MPSLNLHSIANDPDWQFPELSPIINEVRRERRIELACEGYRGDDLLRWRAHHLIVGKR
                     PLGYWFDKDFWTGVQDSEDNYVDGGPLLIPGKDIFVNEDGYLDPYQKNLPNGFGFKPD
                     RDYLYPVPPAQISLNGELTQNPGWE"
     gene            2698..4290
                     /locus_tag="PLI30_07465"
     CDS             2698..4290
                     /locus_tag="PLI30_07465"
                     /inference="COORDINATES: protein motif:HMM:NF024716.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4091 domain-containing protein"
                     /protein_id="HOI79426.1"
                     /translation="MEKILAESSFFPGAQEAISEVVRGEHASLQFVVRSPYTINNLRV
                     TVTQASNKDKLLPASKVRFVGFVKVGRSIWDYSRDRINSPSGYFPDPLLEEDEIDVKF
                     GNSQPIWITIPIPSDAIPGLYRGKVIINGNIENKEFHIEKDYSVRVYPVTIDKTSLWI
                     TNWFTIDAAQLKWMNRGKDFESFSPQHWEYIRKLAKMMAEYRQNSARISPLDLSDFAF
                     INGKWQVDFTRFDKVVEIFIQEGVIGRLEGGHIGTREGNWDSQFVVRVPSVNRNAENR
                     FEKLPLSNERAQQFYVDFFTSLKQHLKEKGWEGSYLQYIADEPTSSNVQSYVEIARFV
                     KQVYPEIKIIEATHSKDLEDIVDIYVPQLDFMNKDYDFYNNINKNSEGKEAWFYTCLS
                     PKGEYANRFIELPLLKTRYIHWLNFKYNIPGYLHWGLNHWRTDPWDEQTSINYEGGNI
                     LPGGDSWIIYPKGDKLLSSIRFEAMRDGIVDYELFKMLEKKNPEAARNIIDKVIYSFD
                     RYDNNIAAFREHRRKLMELLSE"
     gene            4496..>6664
                     /locus_tag="PLI30_07470"
     CDS             4496..>6664
                     /locus_tag="PLI30_07470"
                     /inference="COORDINATES: protein motif:HMM:TIGR04056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0071702 - organic substance transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SusC/RagA family TonB-linked outer membrane
                     protein"
                     /protein_id="HOI79427.1"
                     /translation="MVRSKLSSVFISLIVLFWALSTGAAAQDSTLRGTVKDITGTPLI
                     GVNVIQKGTTNGTVTDVDGNFSINVPANAAIVFTYIGFSAQEVVWDGASAMNVVMHED
                     AELLDEVVVVGYGTQKKVNLTGAVDNMSESRLASLHVNTMGEALQGQLPGVYVSTADG
                     KPGRFSSFNIRGTTSINGGDPLIVIDGVPQSSNSLNNLSPNDIEDISILKDAASATIY
                     GARASFGVILITTKTGKKGKSKVEYNNYFGFSKATIVPEIYDNPVDYIDINQNEFNKY
                     IGQSYFTDAQVEYPYLYEKDPTLPQAKLEIIGGRLTLLLGGKVYNYYKEWLRKYTPQQ
                     NHRISVSGANDSFQYYLAGDYNYEEGNIKFKPEKIKRYTLLSNLRYNLNKNVSLFNNT
                     TMVKRDEEHPNQYLYGFTSNVYRFIENSNAMMPEYVDIDGKMVPTDIGFYRRFLEKQS
                     GIEKDVHDVKTTLGVDISLLNKDLEIRIDGTYQNENTDYLRWWDNVGPYLSNSFNNRN
                     IVLDYYSDAGPAKIYRSNWKSVLTNINAYGTYSKLINDHDIKVMGGYNQEHYDYLYSY
                     AEKLYPLPVDQHSLNLATGASNTRDDDNRNSSRSIFSRLNYNWKSRYLVEFNTSYFLS
                     SRFEKQKRGHGYLSSSAAWRISEEAFFEPLKTVVPNLKLRFSYGSLGNANIGSYDYIP
                     IMSVSQSGFSLDGNLVNLTSAPAPKSDNFTWETVESFNLGL"
BASE COUNT         2092 a         1206 c         1523 g         1843 t
ORIGIN      
        1 tatgtaggac gtaagatagg ggagatctgg ggatataata ccgtgggtat ctatcaaagc
       61 caggaagaag ctgatgctgg ccccgatcaa acatattttt tctcacggtg gggtgagggg
      121 gacatcgaat acgcagattt aaataaggat ggaaaaatta atcccggaaa caatacgtta
      181 gacgatcatg gcgacttgtc tattattgga aactcaactc cgcgctttag ctacggtatt
      241 aatcttggac ttgactggaa gaattttgat ttaagtgttt tttgtcaggg tgttggcaag
      301 cgtgatgcag caccgtcggg taatatgttt tacggaataa caggagacgt atggcaaagc
      361 agtgttttcg ttcaacatct ggattactgg agttcggaaa atccgaacgc ttattatcca
      421 aaaccctatt taggtacgaa tgaacattca aaaaacattc agactcagac tcgttttctg
      481 caaaatgcag cttacttcag gttgaagagt tttcaatttg gatatacact cccggtagaa
      541 aaaaagataa aattcccaat tgagaaaatc agatttttta tcacgggtga aaacttattt
      601 acactaacta aaatgaataa aacatttgat cctgaaactg catttgttgg ggcttacgga
      661 tcgggaaaag cttatcccct gagaagaact ttttcgtttg gattaaatgt tgatttataa
      721 aatacaaaag aattacaagt atgaaaaaaa ttatatattt aatagtattc tcctttacgg
      781 ttctcacaag ttgtgatttg gatgattacc tgaataaaac accgcttgac tccattacag
      841 atgtcgatta ttggaagtct gcatcggacc tgcagctata cgttaatcaa ttttatacga
      901 tgctgcctca atttcccagc tggggaggag gatatctgtg ggaagataac aacagtgaca
      961 atatggcaag ccgcttttat gataatcgtt tagccggaac caacacgatt acgacaaata
     1021 acggaagttg ggattattca cgaatcagga gcatcaatat catgttaaac aaatacaaag
     1081 agatgagtgt ttccgagtct gaaatagctc catatgtggg tgaggcatat ttcttcagag
     1141 catttgcata ctttaatcta gtacgacagt atggggatgt tccgtatgtt tcgcaagtgc
     1201 tgacccctga ttcagaagaa ctttttgcag cacgtacccc ccgtaaagag gttatcgact
     1261 acattctgga tgacctttcc caggcaatag aaaaactacc tcctaaagca gcatcaacac
     1321 gtaatcgcat caataaggag tccgctcttt tatataaatc gcgagtggct ctttatgaag
     1381 gaacatggga aaagtatcat caggaaactg tctttggagt gaagggtagt aatggacagg
     1441 agtacctgga aatcgctgcc tctgcggcga agcagctgat ggacatgaat accatagaac
     1501 tatactccac cggcaatccc gaagttgatt atgcttcatt gtttaaccgt gatgacctaa
     1561 gcggtatagg tgaggttttg ctcacaaggg attataatat ttcgctgaac acagtgcata
     1621 atcttcaacg atatacaacg agcggtgccg gaacgggatt gactaaatcg ctaatagaga
     1681 gctacctgtg cgaggacgga aagcctatat ctttaagccc gttatatagg ggtgacaagt
     1741 cattggaaat ggtagtggag aatagggatc cacgattaag gcaaatgatt tattggaaag
     1801 aaggggagcc cagaagaacc caaaacggag ttctagtacc cggatatgaa tttacccggc
     1861 cttacataga ccagggcgga gaaggaggaa atactacggg ttatatgttg aaaaaggggc
     1921 ttgacaccga atcaaaagga caaccggaac aaaatgcatc ggaaacatcc atgcctatct
     1981 ttcgctatgc agaggtactt ttaaattatg cggaagcaaa agcggagctc gggactataa
     2041 cacaaaacga tatagatttg acaataaaca aaattcgttc aagggcaggt atgccgtctc
     2101 taaacttaca ctccatagcg aacgatcctg attggcaatt tccggaactt tcacccatta
     2161 ttaacgaggt gcgtagggaa aggagaattg aattggcttg tgaaggatat agaggtgatg
     2221 atctattaag atggagagca catcatttga ttgttggcaa acgtcctctt ggttactggt
     2281 tcgacaagga cttctggacg ggagttcagg atagtgagga taactatgta gatggaggcc
     2341 ctttgttaat tcctggaaaa gatatttttg tgaatgaaga cggctacctc gatccgtatc
     2401 agaaaaatct accaaacggg ttcggattca aacccgacag ggattattta tatccggttc
     2461 ctcctgccca aatatcactg aacggtgagt tgacgcaaaa cccgggttgg gagtgagtga
     2521 taaagttttg tctaatttat taaaaaatcg atgaacatga cgttgaaaaa taacacatct
     2581 tatctgtcta attcagtaaa aagaactttt ttgaatgcaa tcattttact atttgtgatt
     2641 tgtttctctg gttttagttg cgacaggaaa gagattcaat ttgaatatgt tgaccctatg
     2701 gagaaaattc ttgccgaaag cagttttttc ccgggtgcac aagaagcaat tagtgaagtg
     2761 gtccgaggcg aacatgccag tctacagttt gttgttcgct caccctatac aataaacaac
     2821 ttaagggtaa cggttaccca agcatcaaat aaagacaaac tgctccccgc atcgaaagta
     2881 agatttgttg gttttgttaa agttgggcga agtatctggg attattcccg ggacagaatc
     2941 aattcacctt cgggttattt tcccgatccg ttattggaag aggatgagat tgatgtgaaa
     3001 tttggtaatt cacagccaat ttggatcact atacccatcc cctcggatgc catccccgga
     3061 ttgtatcgtg gtaaggtgat aataaacggg aatattgaaa acaaagaatt tcatatcgaa
     3121 aaggactatt ctgtcagggt ttatcctgta acaattgata aaaccagttt atggataaca
     3181 aattggttca caatagatgc ggctcaactt aaatggatga accggggtaa agattttgaa
     3241 tctttttctc cacaacattg ggagtatatc cggaaactgg caaaaatgat ggcggaatac
     3301 agacaaaact cggccagaat ttccccactg gatttatctg attttgcatt cataaatggg
     3361 aagtggcaag tagatttcac ccgattcgac aaggtggtgg aaattttcat acaggaaggg
     3421 gttattggca ggttagaagg agggcacatt ggaacaagag aaggtaattg ggattcacag
     3481 tttgtggtga gggtgccttc agtgaacaga aacgcagaaa ataggtttga aaaattgcca
     3541 ttatccaatg agcgagctca gcagttttac gttgatttct tcacttctct taaacaacac
     3601 ctgaaggaaa aagggtggga gggtagttat ttacaatata ttgccgacga acctacgtcc
     3661 tctaacgtac aaagttatgt ggaaattgca cggtttgtca agcaagtgta cccggagatt
     3721 aaaattattg aagctacaca cagcaaagat ctggaggata ttgtggacat atacgtgcct
     3781 caactggatt tcatgaacaa ggattatgat ttttacaata acatcaataa aaatagtgaa
     3841 gggaaagaag catggtttta tacatgcctg tctcccaagg gagaatacgc caatcggttt
     3901 attgagttgc ctttacttaa aacccggtat atacattggt taaacttcaa gtataacata
     3961 cccggttatt tgcattgggg gctcaatcat tggcgaacgg atccctggga tgaacaaact
     4021 tcaattaatt atgaaggagg aaatatttta ccgggaggcg actcctggat aatttatcca
     4081 aaaggtgata aacttcttag ttcaatccgg tttgaagcta tgcgtgatgg cattgtggat
     4141 tatgaacttt tcaagatgct cgaaaagaaa aatccagaag cggctcgtaa tattattgat
     4201 aaagtaattt acagttttga tagatatgat aacaacatag ctgcgttcag ggagcacagg
     4261 agaaagttaa tggaactgtt gtctgaataa actcctcgat atcaccggca atgaaaccgg
     4321 taaatgtttg cccctcaagg cggttcaaaa ctgtcttgag gggtttttat tgggttgtgt
     4381 tcaattctca aatgttaagt aacaaattct tcgttaaaaa gtaaccgctt cttaacatgt
     4441 ataatttttc tttgcgtctc gattggaaat tttaatttga ttaacaaaaa aaattatggt
     4501 tagaagtaaa ttgtcaagtg tgtttataag cttgattgtg ttattttggg ctctttcaac
     4561 aggagcagct gcgcaagaca gtaccctgag gggaactgta aaagacatta ccggcacccc
     4621 gctcataggg gtgaatgtga ttcaaaaagg taccacaaac ggaacggtga cggatgtgga
     4681 cgggaacttc tcgatcaacg ttcccgcaaa cgcggcgata gtctttacgt acatcggctt
     4741 ttcagcgcag gaagttgtct gggacggtgc ttccgcaatg aatgtggtta tgcacgaaga
     4801 tgcagagcta ttggatgagg ttgtagtggt tggttatgga acacagaaaa aagtgaattt
     4861 gacaggagct gtggacaata tgtccgaaag ccgattggcg tctcttcatg taaacacgat
     4921 gggggaggct ttgcaaggcc agctgccggg tgtttatgtg tctactgccg acgggaaacc
     4981 gggaagattc tcgtctttta atatacgggg aactacctct ataaatggag gtgacccttt
     5041 aattgttatt gacggggttc ctcagtcgag caatagtctg aataatttat cgccgaatga
     5101 tattgaagat atatctattt tgaaagatgc cgcttcggca actatctacg gcgcacgtgc
     5161 atcgtttggg gttatcctta tcaccacaaa aacaggaaaa aagggaaaat ccaaggtgga
     5221 atacaacaat tacttcggat tcagcaaagc taccattgtc cctgaaatat acgataatcc
     5281 ggtagattat atcgatataa atcaaaatga attcaataag tacatagggc aatcgtattt
     5341 taccgatgcc caggtggagt atccctactt gtatgaaaag gacccgaccc ttccacaggc
     5401 aaaattagaa ataatcggcg gccgattgac tttgctgttg ggtggaaaag tatataacta
     5461 ttataaagag tggttgcgca aatatactcc tcaacaaaac catcggatca gtgtcagcgg
     5521 agccaacgac tcttttcaat attacctggc gggagattac aattacgaag aggggaatat
     5581 taagttcaaa cccgagaaaa ttaaaagata cactttgttg tctaatttga ggtacaacct
     5641 caacaagaat gtttccttgt ttaataatac gacaatggtt aaaagggatg aggagcatcc
     5701 caaccagtat ttatatggct ttacctccaa tgtatacagg tttattgaga attcaaacgc
     5761 gatgatgcct gaatatgtag atatcgatgg gaaaatggtg cctaccgata tcgggttcta
     5821 cagaagattt ctcgaaaaac aatcgggtat tgagaaagat gtgcatgatg tgaaaaccac
     5881 attgggagtg gatatttctt tgttgaacaa ggatttggaa atacgaatag acgggacata
     5941 ccaaaatgaa aatacagact acctaaggtg gtgggataat gttgggccgt acttgtcaaa
     6001 ttctttcaac aacagaaaca tagtcttgga ttattattcg gatgccgggc cggccaaaat
     6061 ataccggagt aactggaaaa gcgtattgac caacattaac gcctacggta cgtatagtaa
     6121 actgatcaat gaccatgaca taaaggtcat gggagggtat aaccaggaac attatgacta
     6181 tttgtattct tatgctgaaa aactgtatcc acttcctgta gaccaacatt ctctcaacct
     6241 ggcaacgggc gcttcaaaca cgcgggatga cgacaatcgg aattcgtcac gcagtatctt
     6301 ttcccgctta aactacaatt ggaaaagcag gtatctcgtg gaatttaata ccagttattt
     6361 cttgtcatcg agatttgaaa aacaaaaacg gggacacggc tatctttctt catcggctgc
     6421 atggcgaata tccgaggaag cgttcttcga gcctttaaag acagttgtac ctaatctgaa
     6481 attgcggttt tcgtacggct cattggggaa tgcgaatata ggctcttatg actatattcc
     6541 tattatgagt gtaagccaat ccggtttttc gttggacgga aacctcgtta acctgacgtc
     6601 tgctcctgcc ccgaagtctg acaactttac ctgggaaacg gtcgagtcgt tcaatttagg
     6661 tctg
//
LOCUS       DAOSDR010000163         6607 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrimonas sp. isolate AMR_MDS_1826
            NODE_4209_length_6607_cov_3.924298, whole genome shotgun sequence.
ACCESSION   DAOSDR010000163 DAOSDR010000000
VERSION     DAOSDR010000163.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863355
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrimonas sp. (wastewater metagenome)
  ORGANISM  Petrimonas sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Dysgonomonadaceae; Petrimonas.
REFERENCE   1  (bases 1 to 6607)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6607)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:10:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,720
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 35
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6607
                     /organism="Petrimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4209_length_6607_cov_3.924298"
                     /isolate="AMR_MDS_1826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2023866"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1185)
                     /locus_tag="PLI30_07475"
     CDS             complement(<1..1185)
                     /locus_tag="PLI30_07475"
                     /inference="COORDINATES: protein motif:HMM:NF024779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4105 domain-containing protein"
                     /protein_id="HOI79428.1"
                     /translation="MKKYFFALILITMSVFANAQVVLSDSAKISLMTCGPWSGAVYAF
                     YGHTALRVQDDSAHMDIVFNYGFFDPTQPNFMYHFVRGETDYILGTTSFEGFISEYAY
                     KKVAVYEQELNLAQPEKQQLWEALYLNHLPENRGYRYNYFYDNCATRPRDMVEKFAGG
                     KIIYPPTKEGQTYRDLLHECLQPYPWNKFGIDLVIGAGADSPIDVRAKMFIPSYLMNS
                     FEGATVVKNDSVSYPLIRSTTTVLVSNEEDNGSEQIIFSPLIAAFALFFITLLVSIIQ
                     YIKWNKTQLPQIYDTILFAVYGLGGCILFLLMFFSEHPATNPNWNFVWLNIFALVAAI
                     LFWAKPVKKAVNIYHFINFAALTLFLLFWWLLPQQLPVAGILFSMSMWLRSGMNVFMQ
                     TKR"
     gene            1407..2009
                     /locus_tag="PLI30_07480"
     CDS             1407..2009
                     /locus_tag="PLI30_07480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008862803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SagB/ThcOx family dehydrogenase"
                     /protein_id="HOI79429.1"
                     /translation="MNKLVIFLLFSWLTLPLDAQDIQLSAPDKKGGKPLMQALSERKS
                     AREFQEKELPDEVLSNLLWAANGFNREDKRTAPTANNRQELELYLVMKSGIYFYNARE
                     HRLALVKKGDYRKSAGTQTYVANAPVNILFVSDSGKASGKNYAYTDCGFVSQNIYLFC
                     ASEGLATVVRGSYDKKVLEELLQLPANQEVLLTQTVGYPK"
     gene            complement(2094..2348)
                     /locus_tag="PLI30_07485"
     CDS             complement(2094..2348)
                     /locus_tag="PLI30_07485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007212861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HOI79430.1"
                     /translation="MKKEIHPSNYRPVVFKDMSNEEIFISRSTINAKETIEIDGVTYP
                     LVKLEITSSSHPFYTGKQKLVDTAGRVDKFMSRYGDRKKK"
     gene            2527..3779
                     /locus_tag="PLI30_07490"
                     /pseudo
     CDS             2527..3779
                     /locus_tag="PLI30_07490"
                     /inference="COORDINATES: protein motif:HMM:NF014517.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CinA family nicotinamide mononucleotide
                     deamidase-related protein"
     gene            3769..4212
                     /locus_tag="PLI30_07495"
     CDS             3769..4212
                     /locus_tag="PLI30_07495"
                     /inference="COORDINATES: protein motif:HMM:NF012787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HOI79431.1"
                     /translation="MQTKTEKITVKSLDGNTLNLMDIYRGKPLLILFFNIHCLGCVGR
                     AIPLAYDYLQEFKDLNVVAIHTTFGKEMISKDDILNLFTMKELPFPIYFDIEKTNYET
                     FECEGTPHWILLDKEGNPTRSIFGSQDGSQTRLIYALEELTRESE"
     gene            4209..4826
                     /locus_tag="PLI30_07500"
     CDS             4209..4826
                     /locus_tag="PLI30_07500"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019846.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOI79432.1"
                     /translation="MKEFWDNKYSTNEYVYGEEPNEYFKYAIDTFLPAGRLLLPAEGE
                     GRNAVYAVKKGLEVVAFDQSKEGKKKALKLADKAGVKIDYRVGELPDLPLEGEIFTAA
                     ALIYAHFTPQLRLEYHRLIGKLIKPEGLIIVEGFSQQHIEYQKKYPNIGGPRNIGMLY
                     TLEMIRSDFDDFEPIESSEGEVELSEGSSHIGTGNVIRFIGRKVK"
     gene            4969..5559
                     /gene="ruvA"
                     /locus_tag="PLI30_07505"
     CDS             4969..5559
                     /gene="ruvA"
                     /locus_tag="PLI30_07505"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010539156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HOI79433.1"
                     /translation="MIDYIKGEIAELTPASITIECNGIGYMANISLNTYTALNGKQSA
                     KLYIFESIREDAHVLFGFADKHEREIFLHLISVSGVGPSTGRMILSSLSSRELEAVIA
                     SENATVLQSVKGIGAKTAQRIIIDLKDKIKYTEEGSTVKIPAALFISGAALEAVSALV
                     MLGFTKQASEKAVAKIAKESPSLSVEAIIKEALKRM"
     gene            5601..>6607
                     /gene="sprA"
                     /locus_tag="PLI30_07510"
     CDS             5601..>6607
                     /gene="sprA"
                     /locus_tag="PLI30_07510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019539828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell surface protein SprA"
                     /protein_id="HOI79434.1"
                     /translation="MKRTALYNLFLIVLLAVSRIQPVVAQQDTTKSRFPVQKTTVETV
                     EDLTKKLPADLQTPSNIRTEVFYDYKTNRYVFQNKVGDKVTGIPFTMTPAEYMEYTLK
                     ESNDKYFKNRNAIRKEDKPAGKEPLPFFNLRRSNTLLEDVFGPGGIQLTTQGSIELSS
                     GLIRNVIDNPTLPERSRKRTRFDLDPQIQLNVNAKVGNKINFGLNYDTDAAFNFDARR
                     VKLAYQGDEDEIIKNMEAGNVSMTTENSLINGGTALFGIKSDLQFGKLRVSTVLSQQE
                     SESRTISSRGAVQTTPFEINADQYDENRHFFLSHYFRDNYDKALAKLPYVQSAVSITR
                     L"
BASE COUNT         2039 a         1456 c         1414 g         1698 t
ORIGIN      
        1 ccttttcgtc tgcataaata cattcattcc cgatctgagc cacatactca ttgaaaaaag
       61 aatgcctgcc accggtaatt gttgtggcag caaccaccag aaaagcagaa agagggttag
      121 ggctgcaaag ttaataaaat gatagatatt taccgctttt ttcaccggct ttgcccaaaa
      181 taaaatggca gcgacaagcg caaaaatgtt tagccataca aaattccagt taggatttgt
      241 agccggatgc tccgaaaaaa acatcaggag aaacaggata catcctccca agccgtaaac
      301 agcgaacaaa atcgtgtcat aaatttgggg caactgggtt ttattccact ttatgtattg
      361 aataatcgat accagcaagg ttataaaaaa aagcgcgaat gccgctatta acggtgaaaa
      421 tataatctgt tcgctaccat tgtcctcctc atttgaaacc aataccgtag ttgtacttct
      481 gataaggggg tagctgacag agtcgttctt aacaacggtt gccccctcaa atgaattcat
      541 caaataagaa ggaataaaca tttttgcacg aacatcaata ggggaatcgg ctcccgcacc
      601 tatgaccaaa tcaataccga atttgttcca cggatagggt tgtaaacatt cgtgcaagag
      661 atcccgatag gtttgtcctt cctttgtggg tgggtatata atcttacctc cggcgaattt
      721 ctccaccata tcgcgcggac gtgtggcgca gttgtcgtaa aaataattat accgataccc
      781 cctgttttcc ggcaaatgat tgaggtacaa ggcctcccat aactgttgct tttccggttg
      841 agctaaatta agctcctgtt cataaacggc gaccttcttg taggcatatt cgctgataaa
      901 tccttcaaaa gatgttgttc ccaagatata atcggtctcc ccccttacga agtgatacat
      961 aaaattgggc tgggtagggt caaaaaaacc gtaattgaat acaatatcca tgtgtgcgga
     1021 atcgtcctgt acacgcaacg ccgtatgtcc gtaaaacgca taaactgctc ccgaccatgg
     1081 cccgcaggtc atcaggctta ttttggcact gtcgctcaat actacctggg catttgcaaa
     1141 aaccgacatc gtgataagaa tcagggcaaa aaaatatttt ttcattatcg actgaactta
     1201 tgttggaaat gcaaatttac atataaaagt ttattttcac ggcttttcaa tcaatggaaa
     1261 aaccacagga cctccaccta cctctcggtt tctgacacaa cctatgcctg aaaaatggga
     1321 taatccgcta tattttaacc tgtttttaaa ccatacgttc ggcatttttg gctatatttg
     1381 tacgtcgtaa atctaaaatt ttaagcatga acaaattggt tatttttctt ttattttcat
     1441 ggctgacact acccttggat gctcaggaca ttcaactctc cgctccggat aaaaaaggcg
     1501 gaaaacccct aatgcaggcg ttgagtgaac gcaaatccgc ccgtgagttt caggaaaaag
     1561 aactgccgga cgaggttcta tccaatttac tttgggcggc caacggattt aaccgcgaag
     1621 ataagcgtac tgccccaaca gccaacaaca ggcaggaact cgaactttac ctggtgatga
     1681 aaagcggtat ttatttttac aatgcccggg aacatagact ggcgttggta aaaaaaggcg
     1741 attataggaa aagtgctggt acacaaacct atgttgccaa cgcaccggtg aatatccttt
     1801 ttgtttccga ttccggaaaa gcatcgggca aaaactatgc atacaccgat tgcggctttg
     1861 tttcgcaaaa tatttatttg ttctgcgcct ccgaaggatt ggcaacggtt gtacgcggtt
     1921 cgtacgacaa aaaggtactt gaagagctcc tgcaattacc ggcaaatcag gaagtgctgc
     1981 tcacacaaac ggttggctat cccaaatagt ttgtcggctt cgcattgttc gttaacaaaa
     2041 acggtgattc tctttcgaaa accaccgttt tttattatta cctgaaaaag gatttatttt
     2101 ttcttacgat ctccgtaacg gctcataaac ttgtctacac gtccggctgt atccaccaat
     2161 ttttgttttc cggtgtaaaa cggatgcgat gaactggtaa tttcaagttt caccaaagga
     2221 taagtaacac cgtcaatctc aatggtttcc ttggcgttta tagtagaacg tgaaataaaa
     2281 atttcttcgt tcgacatatc tttgaaaact accgggcgat aattgcttgg atgtatttct
     2341 tttttcattg ttatatgatt tgttttattt ttgttgcttt ggattttcgg aatgcaaaga
     2401 tataacaatt cattgaaata gaaaaattaa atagaaataa tttagaaaaa atcgattcct
     2461 atagcttaat tttgtacgaa attggaaagc taaaaagaaa caagcatgta aatcggttac
     2521 acacaaatga aaatagagat tataaccgtt ggagatgaaa tccttatcgg gcaaatcatt
     2581 gataccaatt ccgcctggat ggcagcagaa ctcacccggc agggatttga aaccgtggca
     2641 attacaaccg tcggcgacca ggaagaagat atggcgaaag ctattgacac aggcttttcc
     2701 agggcggatg tactgttgat gacgggggga gttggaccca cccgggacga tattaccaag
     2761 aaaacactct gcaactactt ccgtaccgaa ttaattttca acgaaaaagt ctttcgacaa
     2821 atcaaccgtc tctttgcaca caaaaattat ccgatgaatg aattgacaaa gaaccaggct
     2881 tttgttccga aaggttgtac tgttatacag aatagagtcg gaacagcccc ccttctttgg
     2941 tttgaacaaa gaagccacat attggtttca atgccaggtg ttcctttcga gatgaaaaca
     3001 gcagtaacca ctgaaatcat tccccgatta aaaaaacgat tcctatcggt tgactacctg
     3061 aagtcttcat tcctcgtatc gggaatcacg gaatcgtcat tggccatcct gctggaagac
     3121 tttgaagcga cacttccagg caatttctca ctcgcctatc tcccctccta cggattgatc
     3181 cgcttacgac tgtcggcccg gggcaaagag aaccttccgg aaatgaggtt tcaagagaaa
     3241 aaactcaggg aagtgttgtg cgatttcctt gttgctgata gtgatgaacc gcttgaagca
     3301 ttgctgggtg aagctttgag aaaagaaaag ctcaccctct ccacagccga gagttgcacc
     3361 ggaggaaaca ttgcccaccg gataacgctt gttccgggcg cgtcggagta ttacaaaggt
     3421 acggtttatc gtacgacaac agagttaaaa tctccctgct ggaggtttca aaccaagcaa
     3481 ttgaaacgta cggtgccgtc agccgcgaag tagtggagca aatggcgcag aatgtatctg
     3541 aaaaaatgaa gaccgactgc tccatagccg tatcaggtat tgccggccct gatggaggca
     3601 cacccgaaaa accagtggga acggtatgga tttgcacccg gatgcatcgc gaaacggtaa
     3661 cgaaaaaata tcaattcggt acttcccgcg aagaaaacat aaacagaaca accaacatgg
     3721 ctatcctaca gatgataaaa atgcttgaca aatataaaaa caaaaaagat gcaaactaaa
     3781 acagaaaaaa tcaccgtaaa gtctctggac ggcaacacgc tgaatttaat ggatatttat
     3841 cggggaaaac ctctgctaat cttgtttttc aacatccatt gcctgggatg tgtgggcagg
     3901 gcaatcccct tggcctacga ctacctgcag gaattcaaag acctgaacgt agtggccatc
     3961 cacaccactt ttgggaaaga gatgatctcc aaagacgaca tcctcaactt atttacaatg
     4021 aaggagctac cattccctat ctattttgac attgaaaaaa caaattacga aacgtttgaa
     4081 tgcgagggca caccccattg gattctgttg gataaagaag gcaacccgac ccggtcaatt
     4141 ttcggatcgc aggatggctc ccaaacccgc ttgatttacg cactcgaaga attaacacga
     4201 gaatcggaat gaaagaattc tgggacaaca aatattccac aaatgaatat gtttacggag
     4261 aagaaccgaa tgaatacttc aagtacgcaa tagatacttt tctaccggcc ggaagattac
     4321 ttctaccggc cgaaggagaa ggtcggaatg ccgtgtatgc tgtcaagaaa ggcttggagg
     4381 tagtcgcgtt cgaccaaagc aaagaaggaa agaagaaagc cctaaaactg gctgacaaag
     4441 ccggtgtaaa aattgattat cgagtaggtg aacttcccga tcttccgttg gagggagaaa
     4501 ttttcaccgc tgccgctctt atttatgctc attttacccc acaactgcgt ttggagtatc
     4561 accggttgat cggtaaactt atcaagcccg agggtcttat tattgtggag ggattcagcc
     4621 aacagcacat cgaatatcag aaaaaatatc ccaatatcgg tgggccacga aatatcggga
     4681 tgttgtatac cctggagatg atccggagtg attttgacga ttttgagccc atcgaatcaa
     4741 gtgaaggaga ggtggaactt tccgaaggaa gcagccacat aggtaccggt aacgtaatcc
     4801 ggttcatagg aaggaaagtc aagtgaaaac ctactgagtt gcctgtgtcc ggaaaggttg
     4861 ttttaaggtt tttgcttata aacgattttt ctccccctga tttatacgtt ttaaccaaaa
     4921 tttccgttat atttactgaa tcgagtatcg ttacaacttt ttgaatttat gattgactat
     4981 atcaaaggtg aaatcgctga gttaacacct gcttccatta caattgaatg caatggcatt
     5041 ggctatatgg caaatatttc cctgaacacg tacaccgcac tcaacggcaa acaatcggcc
     5101 aagctttaca tttttgaatc catccgcgaa gacgcacacg tgctttttgg ttttgccgac
     5161 aaacacgaac gcgaaatttt tctacacctt atcagcgtat cgggtgtcgg cccaagcaca
     5221 ggacgaatga ttttgtcgtc actcagttcc cgtgaactgg aagcggttat tgcttctgaa
     5281 aacgcgactg tactccagtc tgtaaagggt atcggtgcaa aaaccgcaca acgcatcatc
     5341 atcgatctga aggataagat caagtatacg gaagagggtt caacggtgaa aataccggct
     5401 gccttgttta tctcgggagc ggccctggaa gctgtttctg cactggtgat gctaggattt
     5461 accaaacaag cttcggaaaa ggcagtggca aaaatagcga aagaatcacc gtcactatcg
     5521 gtagaggcca ttattaaaga ggcgttgaaa cggatgtaaa atgagttgtg agttatgtta
     5581 tctatccact cactggataa ttgaaaagaa cagcattata taacctcttt ctcattgttc
     5641 tgcttgctgt gagcagaata caacctgttg ttgctcagca agacaccacc aaatcaaggt
     5701 ttccggtgca gaaaacgacc gttgaaacgg tggaagacct aactaaaaaa ttacctgcag
     5761 atctccaaac accctcgaac ataagaacgg aagtgtttta tgattacaaa accaaccggt
     5821 atgttttcca aaacaaagtc ggcgataagg taacaggaat tccatttacc atgacgcctg
     5881 cggagtacat ggaatatacg ttgaaagaat cgaacgacaa gtatttcaaa aatagaaatg
     5941 ccatcaggaa agaggacaaa cctgcaggca aggagccgtt accgtttttc aacctacgtc
     6001 ggagcaacac gctgctggaa gacgttttcg ggcctggcgg aatccagttg accactcagg
     6061 gatcgataga actcagcagc gggttaataa gaaacgtaat cgacaacccc accctccccg
     6121 agcgctcccg aaagcgaacc cggttcgatc tcgatccaca aattcagctt aacgtcaatg
     6181 ccaaagtcgg caacaagatt aatttcggac tgaattacga taccgatgct gcgttcaatt
     6241 tcgatgccag acgtgtaaaa ctcgcttatc agggtgatga agacgaaatc atcaaaaaca
     6301 tggaagccgg aaacgtgagt atgaccaccg aaaactcgct tatcaacggc ggaacggctc
     6361 ttttcggtat caaatcagac ctacagttcg gcaaattgcg cgtaagcact gttttgtcgc
     6421 aacaggagtc ggaatcccgg accatcagtt ctcgcggtgc cgttcaaacc acacctttcg
     6481 aaattaatgc cgatcagtac gacgaaaacc gtcatttctt tttaagtcat tacttcaggg
     6541 acaattacga caaagcactg gcaaaattgc cttacgtcca atcggcggtc tcgataaccc
     6601 ggttaga
//
LOCUS       DAOSDR010000164         6602 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrimonas sp. isolate AMR_MDS_1826
            NODE_4214_length_6602_cov_3.776081, whole genome shotgun sequence.
ACCESSION   DAOSDR010000164 DAOSDR010000000
VERSION     DAOSDR010000164.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863355
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrimonas sp. (wastewater metagenome)
  ORGANISM  Petrimonas sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Dysgonomonadaceae; Petrimonas.
REFERENCE   1  (bases 1 to 6602)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6602)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:10:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,720
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 35
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6602
                     /organism="Petrimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4214_length_6602_cov_3.776081"
                     /isolate="AMR_MDS_1826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2023866"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2017
                     /locus_tag="PLI30_07515"
     CDS             <1..2017
                     /locus_tag="PLI30_07515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019539485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HOI79435.1"
                     /translation="VYVYQKKYLPGALLIMIFVAFQISANHVQMSYYFFFLMFFMVAA
                     FLVQAVREKKLAGFLKSTVVVVIAGMIGVLANSSNLYHTYEYSKETMRGKSELSHHGA
                     ENKSKNGLERDYITGWSYGVDETWTLLVPNTKGGSSSARMSENLKAMEKARPEYRELY
                     KQIAQYWGEQPWTAGPVYVGAFVLMLFILGLFIVKGPVKWALLAGTLFSILLSWGKNF
                     MPLTDFFIDYIPMYNKFRTVSSILVVAEFCIPLLATLAVKEIVQKPEILKKNMKYVGI
                     SWALTGGMALLFWLLPELFFPSYISNFEMQQLQSLPTEHVQTVIGNLTEMRISIFRAD
                     AWRSFYIILGGVLMLIAFVSGKLKAQWMVTGILLLCLADMWTVNKRYLNDNDFTPKSN
                     EQQMFAQTPTDLHILQDTTKYYRVLNMATSTFDDGVTPYYHKTIGGYHAAKLRRYQDL
                     IDIHLIKEMGVLQQDIIRTQGTMDSVNADGYKVLNMLNAKWIIMPAQGGTVPVENPYA
                     MGNAWFVDNIQFVNNADEEIDALAAIDLSRQAVADKKFESVLQGFNVSTADSASTITL
                     ADYDSNFITYTVDAKKDELAVFSEIYYPRGWEITIDGQPAQMLRANYTLRALPISAGT
                     HKVEFRFEPASIKVTDAVAFAALVVMLLTAVWIVFSEIKQNKRRQKQ"
     gene            2014..2310
                     /locus_tag="PLI30_07520"
     CDS             2014..2310
                     /locus_tag="PLI30_07520"
                     /inference="COORDINATES: protein motif:HMM:NF019749.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HOI79436.1"
                     /translation="MKKSIFYLFIVLMLSACGSVVEMTSSKMNRLELGMSKEEVTHIL
                     GNGYTIAEKRIEAGKQMEILSYRDFYKDEEFYMFVFEDNRLKEWYRDLLPKVEK"
     gene            complement(2360..3127)
                     /locus_tag="PLI30_07525"
     CDS             complement(2360..3127)
                     /locus_tag="PLI30_07525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019541154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-type zinc ribbon domain-containing protein"
                     /protein_id="HOI79437.1"
                     /translation="MATRKKSTVEDIAVDEKLKSLYKLQSYLSEIDKIKTLRGELPLE
                     VADLDDEIAGLGTRINKFELDLKELSDMTKQEKFKIETSKAKIEKYNKQLENVRNSKE
                     FDHLSKEVEFETLEIELAEKHIRENTQSEKLIKEQITAAQEQLKEKNFDLEAKKKELD
                     DIVSETKAQEEKIREKAKKTETTIEPRLLTAFKRIRKNARNGLGIVPIQRGACGGCFN
                     KIPPQKQMDIKMGKKIIVCEYCGRIMIDPELAGVTTE"
     gene            complement(3295..4389)
                     /locus_tag="PLI30_07530"
     CDS             complement(3295..4389)
                     /locus_tag="PLI30_07530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010538027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051540 - metal cluster binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HOI79438.1"
                     /translation="MRIKEIVQTIENLAPVPLQEDFDNSGLQVGDINREATAALLSLD
                     VTESVIDEAVSLGCNLIISHHPLAFKPFKSLTGRTYIERCMIKAIKHDIVIYAAHTNL
                     DNASQGVNFKLAEMLGLQQLRILSPKKDSLLKLVTFVPESHAEYVRSALFNAGAGNIG
                     NYDSCSFNLHGEGTFRANELANPFVGNTGKFHFEKEVRIETVLPRFKQADVLRALLSV
                     HPYEEPAYDFYPLKNDWAQVGSGVVGVLPEPIPEQEFLYLLKDVFNLPTIRHTKLQNR
                     EIHDVALCGGAGAFLVPEAIAYGADAFITGEARYNDFYDVDSHLLLAVVGHYESEICT
                     KEIFFDVISKKFPTFVVHKSAFDSNPVKYL"
     gene            4464..5336
                     /locus_tag="PLI30_07535"
     CDS             4464..5336
                     /locus_tag="PLI30_07535"
                     /inference="COORDINATES: protein motif:HMM:NF014625.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HOI79439.1"
                     /translation="MKYLIIAFFLFLPFSLVLSQDKKINVIVIGAHPDDPDGTAGGTA
                     IKFAELGHNVLFVSLTNGDAGHQSKGGGALAKIRRGEAQEAGKRLGVTYRVLDNHDGE
                     LLPTLEVRHQVIRLIREWEADIVIGPRPYDYHPDHRNTALLLQDAAYMVIVPNVASDT
                     PPLKRNPVFLYAQDRFQKPYPFIPDIAIDISDVLDRKIYAMAAHESQYFEWLPWTVSQ
                     EQNVPKKEKERIEWLKSGRRRTIIPAVKEALEKWYGKEKADKITDAEAFEICEYGRQP
                     NESEIRELFPMIGK"
     gene            5372..>6602
                     /locus_tag="PLI30_07540"
     CDS             5372..>6602
                     /locus_tag="PLI30_07540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009276786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PNGase F N-terminal domain-containing protein"
                     /protein_id="HOI79440.1"
                     /translation="MRKLLITVVLLLLFPLAFYALNRTKKTVHLPVFQQTPILFNPTD
                     YPDGLVEKEGLIYLGNGRIVLKKVRVPQFKNYTEVEIAVTLVSNGDPWDKSGSCFVIP
                     KSSKITMIDIARNSAAYPQQDTVKHESLIGIVQGKDYLPTVELMRFMTPFGVGHFSRN
                     DDPVSVKRRPVYVDGWAENVIWKQEISDLLPLLEDEAYIGVYIDTWTKEGYRIDVQLS
                     FTESNLRGDKKPHLHVEPLINTNYYVGQRHPDIFSRRDVEVPFIIPEKAKNIRLKYIA
                     TGHGGHSGGDEFRPQRNILKIDGSEVLNFLPWRTDCASFRRFNPTSGVWLQKRTMAYI
                     SNEGKRAEKEIEEPLASSDLSRSNWCPGSDVSPIEVELPNLSAGSHSLTISIPEARPI
                     EENKLNHWLVSAYLVWEE"
BASE COUNT         1837 a         1500 c         1462 g         1803 t
ORIGIN      
        1 ggtatatgta taccaaaaaa aatacctgcc aggggcattg ttgatcatga ttttcgtagc
       61 attccagatc tcggcgaacc atgttcagat gagttattat ttcttcttcc tgatgttttt
      121 tatggtagca gcctttcttg tgcaagctgt tcgtgaaaaa aaactcgctg gttttttgaa
      181 atcaactgtc gtggttgtca tcgcagggat gatcggtgta ctggcaaatt cctccaactt
      241 gtaccacacg tatgaatact ccaaagaaac gatgcgcggg aaatcagaac tttcacacca
      301 cggagctgaa aataaatcaa aaaacgggtt ggagcgtgat tacataacag gatggagcta
      361 cggagtagat gaaacctgga cactcctcgt tccaaacacc aaaggcggat cttcttctgc
      421 ccggatgtcg gaaaacctca aagcgatgga gaaagcacgc cctgaatacc gcgagctgta
      481 taaacaaatc gcacaatact ggggtgaaca accgtggacg gcggggccgg tttacgtggg
      541 ggcttttgtt ttgatgctgt ttattctcgg cctttttatc gtgaaagggc ccgtaaaatg
      601 ggcgctactg gcaggaaccc ttttttccat actgctctcg tgggggaaaa attttatgcc
      661 acttaccgat ttctttatcg actatattcc catgtacaac aagttccgga cggtttcttc
      721 aatcctggta gtggctgaat tttgtattcc cctgcttgca acccttgcgg taaaagaaat
      781 tgtccaaaag ccggaaatcc ttaaaaaaaa catgaaatat gtagggataa gctgggctct
      841 cacgggtgga atggctcttc tcttttggtt gttgcctgaa ttatttttcc cctcttatat
      901 ttccaacttc gaaatgcagc aactgcaatc gttgcccaca gaacatgttc agacggttat
      961 cggaaacctc acggagatgc gcatatccat cttcagagca gatgcctggc gtagttttta
     1021 catcattcta ggaggggtct tgatgctgat cgcttttgtg tccggcaaat taaaagccca
     1081 atggatggtg accgggatac tacttctttg tctagccgac atgtggacgg taaacaaaag
     1141 atacctcaac gacaacgact tcacccctaa atcaaatgag caacagatgt tcgcacaaac
     1201 accaaccgac ctgcacattt tgcaagacac caccaaatat tatcgggttc tgaatatggc
     1261 aaccagcaca ttcgacgacg gagttacgcc ctattatcac aaaaccattg gcggttatca
     1321 cgcggctaaa ctgcgccgat accaggacct gatcgatata cacctgatca aggagatggg
     1381 agtgttgcaa caagatatca tccgtacgca gggcactatg gactccgtaa atgccgacgg
     1441 gtacaaggta ttgaatatgc tcaacgccaa atggatcatt atgccagctc aaggcggaac
     1501 cgttccggta gagaatcctt acgctatggg gaatgcttgg tttgttgata acatccagtt
     1561 cgtaaacaac gcagatgaag agatcgatgc gttagcggcg atcgatttaa gcaggcaagc
     1621 cgtggccgac aaaaaattcg agtctgtctt gcagggattc aacgtatcga cagccgattc
     1681 ggcatccacc atcacgttgg ccgattacga ttcaaatttt attacctaca cggtagatgc
     1741 gaagaaagac gaattggctg tcttttcgga aatttactac cccaggggat gggaaattac
     1801 cattgacgga caaccggccc agatgctacg cgctaattat acgttgcgag ccttgcccat
     1861 ttccgcggga acacacaaag tggaattccg ttttgagcca gccagcataa aggttacgga
     1921 cgcagttgca tttgcggcgt tagttgtgat gttgcttacg gcagtatgga ttgtatttag
     1981 cgaaataaaa caaaacaaac gaagacaaaa acaatgaaaa aatcaatctt ttacctcttt
     2041 attgtcctga tgctttccgc atgcggaagc gttgtagaaa tgacaagttc caaaatgaac
     2101 cggcttgagc tgggtatgtc gaaagaagag gtcacccaca ttttagggaa tggatacact
     2161 atcgccgaaa aacggattga agccgggaag caaatggaaa ttctgtctta tagggatttt
     2221 tataaagacg aagaatttta catgtttgta ttcgaggata acagattaaa agaatggtat
     2281 cgtgatcttt tgccgaaagt agaaaaataa gaaagacaaa acagggtttt caaatctgca
     2341 aaccctgttt tgtatagatt tattccgtcg taactccggc taattcaggg tcaatcataa
     2401 tccgtccgca atattcgcac acaataatct tcttccccat cttaatatcc atttgtttct
     2461 gaggcggaat tttattgaaa caaccgccgc aggcaccgcg ctgaataggt actattccca
     2521 agccgttgcg cgcattctta cgaattcgct taaatgcggt aagcaggcgg ggctcaatag
     2581 ttgtttcggt ctttttcgct ttttcccgga tcttttcttc ctgagctttg gtttccgaaa
     2641 cgatgtcatc gagttccttc tttttggctt ccagatcgaa attcttttct ttcagttgtt
     2701 cctgcgctgc agtaatctgt tctttaatca gtttttcaga ctgcgtattt tcgcggatat
     2761 gcttttcggc caattcaatc tcaagcgttt caaactccac ctctttcgat aaatggtcga
     2821 actccttgct gttgcgaaca ttctcgagct gcttattgta cttctcaatt tttgctttgc
     2881 tggtttcgat tttgaacttt tcctgcttgg tcatatcact cagttctttc aaatcgagtt
     2941 caaacttatt gatacgtgtt cctaacccag cgatttcgtc gtcaaggtct gctacttcga
     3001 gaggaagttc acccctaagt gttttgattt tgtcaatttc cgaaagatag gattgcaact
     3061 tatacaacga tttcagtttt tcgtctactg caatatcttc tacggtactc ttttttcttg
     3121 ttgccataat tatattattt attttagatt tcagaccgcc ttgcttagag atttcagata
     3181 ttagacagtc cggcgtctaa tgtcttttat aaaaaaatgc agtaaccaaa cgaggattat
     3241 cgtttcatca ctacattacc tgcaactgcg ggaacaatgt ctgaccgctt tcttttacag
     3301 gtatttcacg ggattcgaat cgaacgccga tttatgtacg acaaaggtag gaaatttttt
     3361 cgaaattaca tcgaaaaaga tttcttttgt gcaaatttct gattcgtaat gtcctacaac
     3421 tgcgagcaat agatgactgt ccacgtcgta aaaatcgtta tacctggctt cccctgtaat
     3481 aaatgcatcg gcaccgtatg caatggcttc gggaacaaga aatgcgccgg cacctccaca
     3541 caaagccacg tcatgaattt cccggttctg caatttggtg tgacggatag ttggcaaatt
     3601 gaatacatct ttcaacagat acaaaaactc ttgctccggg ataggttcgg gtaatacacc
     3661 cactaccccg ctcccaactt gtgcccagtc atttttgagc ggataaaaat cataagcagg
     3721 ctcttcatag gggtggactg acagcaaggc tcgtaacacg tctgcctgct tgaatctggg
     3781 taaaacggtt tcgatcctta cttctttttc gaaatggaat ttccccgtat tgcccacaaa
     3841 agggtttgcc aactcgtttg cccggaaagt accttcgccg tgtaaattga aactgcacga
     3901 atcataattg ccaatatttc cggcaccggc attgaataaa gcgctccgga catactcggc
     3961 gtgcgattca ggaacaaagg taaccagttt cagcaaggag tcttttttag ggctcaaaat
     4021 ccggagttgc tgcaacccta gcatttctgc cagtttgaag ttcactccct gagaagcgtt
     4081 atcgaggttg gtgtgcgcgg cataaatcac tatgtcgtgt ttgatagcct taatcatgca
     4141 tcgctctata taggtccgtc ccgtcaacga tttaaacggc ttaaaggcta gcggatgatg
     4201 cgaaataatc aggttgcatc ccagcgaaac agcttcgtca atcacactct ccgtcacatc
     4261 gaggctcagc aaggctgctg tggcctcccg atttatgtcg ccgacctgaa gcccgctgtt
     4321 atcaaaatct tcctgtaacg gaacgggagc cagattttct atcgtttgca ctatttcttt
     4381 tattcgcata acaaatacct gttttatctg caaatatact tcaattttcg tatttttgtg
     4441 ataaacaaac aaattagctt attatgaagt acctcatcat tgcctttttc ctgtttctcc
     4501 ctttcagcct tgtgctgagt caggataaaa aaataaatgt catcgtaatt ggcgcgcacc
     4561 ctgatgaccc cgacggaacg gccggcggaa cagccataaa gtttgcagaa ctcgggcaca
     4621 acgtattgtt tgtatcgctc acgaacggag acgccggaca tcaatcaaag ggcggaggag
     4681 cgttggcaaa aattcgcaga ggcgaagcac aggaagccgg aaagcgattg ggagtgacat
     4741 acagggttct cgataatcac gacggcgagt tgctccccac cctcgaagtg cgtcaccagg
     4801 taatccggct gattcgtgaa tgggaggccg acatagtaat cggcccccgc ccgtacgatt
     4861 atcatcccga tcaccggaac acggccctgt tgttgcaaga tgcagcctac atggtgattg
     4921 ttcctaacgt ggcatccgat actcctccct tgaaaagaaa tcccgttttc ctttatgcac
     4981 aagaccggtt tcaaaagcct tatccgttta ttccggatat tgccatcgat atttccgacg
     5041 tcctggaccg gaaaatttac gcaatggcgg cacacgaatc ccaatatttc gagtggttgc
     5101 cgtggaccgt ttcgcaagaa caaaacgttc ctaaaaaaga aaaagaacgg atcgagtggt
     5161 tgaaatccgg cagaagaaga acgataattc ccgcggtgaa agaggctctt gagaaatggt
     5221 acggaaagga aaaagcagac aagattaccg atgccgaagc ctttgaaata tgcgaatacg
     5281 gaagacaacc caacgaatcc gaaatcaggg aattatttcc catgattggt aaataaaact
     5341 tcaaaaacag acaacacgaa accatctatt tatgagaaaa ctattgatca ctgttgtgct
     5401 cctgttactt tttccgctgg ctttttatgc gttgaacagg acaaaaaaaa ctgttcatct
     5461 gcctgttttt caacagacac ccattctttt taatccgacg gactatcccg acggcctggt
     5521 agaaaaagag ggactgatct atttgggaaa cggacgtatt gtactgaaaa aagttcgcgt
     5581 tccccaattc aaaaattata ccgaagtcga aattgccgtt acgctcgttt caaatggtga
     5641 tccctgggac aaatcggggt cttgctttgt gattccaaaa tcttcaaaaa tcacaatgat
     5701 cgatatcgcc cgcaattcgg ccgcctatcc gcagcaagac acggtgaaac acgaatcttt
     5761 gatcgggatt gtacaaggga aggattattt gccgacagta gaattgatgc gattcatgac
     5821 acctttcgga gtgggacact tcagccggaa tgacgatccg gtatcggtca aacgacgccc
     5881 ggtttatgtg gatggatggg cggaaaacgt tatatggaaa caggaaatat cggatttgct
     5941 gcccctgctc gaagacgaag cctatatagg cgtttatatt gacacctgga caaaagaagg
     6001 ttaccgcatt gacgttcaac tctcctttac cgaaagtaat ttgcgcggtg acaaaaaacc
     6061 gcatctgcat gttgagccgt tgataaatac caattattac gtcggacagc ggcatcccga
     6121 tattttctca cgaagggatg tggaggtccc gtttatcatt ccagaaaaag caaaaaatat
     6181 ccgcctaaaa tatattgcta ccggacacgg cggtcattcc ggcggagatg agttccgtcc
     6241 tcagcgaaat atactgaaga ttgatggctc ggaggtgttg aattttctgc cctggcgaac
     6301 ggattgtgct tcgtttcgac gtttcaatcc cacatccggt gtatggctgc aaaaaaggac
     6361 aatggcttat attagcaacg aaggtaaacg tgccgaaaaa gaaatcgaag agccgctcgc
     6421 ttcatccgat ttatcacgaa gcaactggtg tccaggtagt gatgtttccc ccatagaggt
     6481 cgaactgccc aatctgtccg caggtagtca cagtctgacc ataagcatcc ccgaagcaag
     6541 gcccattgag gaaaacaagt tgaaccactg gcttgtttcg gcatatttgg tgtgggaaga
     6601 at
//
LOCUS       DAOSDR010000165         6558 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrimonas sp. isolate AMR_MDS_1826
            NODE_4247_length_6558_cov_4.373674, whole genome shotgun sequence.
ACCESSION   DAOSDR010000165 DAOSDR010000000
VERSION     DAOSDR010000165.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863355
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrimonas sp. (wastewater metagenome)
  ORGANISM  Petrimonas sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Dysgonomonadaceae; Petrimonas.
REFERENCE   1  (bases 1 to 6558)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6558)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:10:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,720
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 35
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6558
                     /organism="Petrimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4247_length_6558_cov_4.373674"
                     /isolate="AMR_MDS_1826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2023866"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..561)
                     /locus_tag="PLI30_07545"
     CDS             complement(<1..561)
                     /locus_tag="PLI30_07545"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HOI79441.1"
                     /translation="MTNPDSLIFDMDGTLWDNVDSYVIVWNKGLEINGYRSRVTRDDL
                     LGLMGKEARVMLDAVLPSASRAAQDELFDEVVRQYHLLVPVMKPHIYEGVYEGLEKLS
                     KKYPLFLLSNCEKGGLVNFMKHTGTAHLFTDYMEHGENLKPKSFNMKLLKERNNLQFP
                     LYIGDTDSDSKASSEADVPFVFVTYGF"
     gene            800..1750
                     /locus_tag="PLI30_07550"
     CDS             800..1750
                     /locus_tag="PLI30_07550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019540395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HOI79442.1"
                     /translation="MEKTRVCVIINPVSGTESKKNIPEEVASAFDQTQFDVIIRITGY
                     PGHATEIAKQAVKNKFAYVLTVGGDGTVNEVAKALVNTDTILGIIPFGSGNGLARDLH
                     ISIDSEKAIQTVLEGNVRTIDYGSANGHIFFCTCGVGFDAFISDKFAEEKKRGPLGYV
                     RNIVEGVVEFRSEEYEITHDEGTLNERAFLLTCANASQYGNEVYIAPGADMEDGKMNV
                     SILKPLNPNEIPQTTLQLFTRNIDKNSKMISLVTSNLIIKRKKPGILHVDGEPVNTEK
                     DIEVKMYHKGLKVLAPKQVEEKPVKERENIFTAITRWMNI"
     gene            complement(1842..2237)
                     /locus_tag="PLI30_07555"
     CDS             complement(1842..2237)
                     /locus_tag="PLI30_07555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018358301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF302 domain-containing protein"
                     /protein_id="HOI79443.1"
                     /translation="MDKMFFESKSRYAFDETVERLNNIIAEAGWRVTHTHDLKETMNK
                     NGYEVLPVKVIELCNPKYAYRMLSADELRIYGNMMPCRISVHEKADGKTYVSRMNSAL
                     FASQIGGVVEEVMSGAYHDAESFVAQIAE"
     gene            complement(2250..2750)
                     /gene="trxA"
                     /locus_tag="PLI30_07560"
     CDS             complement(2250..2750)
                     /gene="trxA"
                     /locus_tag="PLI30_07560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006800273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HOI79444.1"
                     /translation="MKIKLIIAALVTSFMFAACSNNTGKSTSSPQAAVTQQETNLNKT
                     NMTKTIHLTKADFLTKVANYEKNSQTWEYLGDKPAIIDFYADWCGPCKMVAPILEQLA
                     AEYEGQIYIYKVDTEAEQELAADFGIRSIPTLLFVPMNEAPQMAQGALPKDAFKQAID
                     EVLLKN"
     gene            complement(2779..3003)
                     /locus_tag="PLI30_07565"
     CDS             complement(2779..3003)
                     /locus_tag="PLI30_07565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018109362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6132 family protein"
                     /protein_id="HOI79445.1"
                     /translation="MKEFFTKHGLKVAGVIVGAIGGYLYYYFVGCGTGSCPITSNPWR
                     MTIYGAILGLLIFDIFKKEEGRGKEIKNIK"
     gene            complement(3016..3330)
                     /locus_tag="PLI30_07570"
     CDS             complement(3016..3330)
                     /locus_tag="PLI30_07570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019538789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HOI79446.1"
                     /translation="MEFSMLEIKEQKVDKAQFEEAAYMLKALANEIRLSVITLLSNEK
                     EKSVSELQEAIDCEQSLLSYHLTDMRAKGILNCRRSGKNCFYSIRNNRVIQLLSCIID
                     CN"
     gene            complement(3470..4162)
                     /locus_tag="PLI30_07575"
     CDS             complement(3470..4162)
                     /locus_tag="PLI30_07575"
                     /inference="COORDINATES: protein motif:HMM:NF024895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin family protein"
                     /protein_id="HOI79447.1"
                     /translation="MKRLLPGILLFILSSVQVFAQKNDFEKEFYIGVGGGALMSTVDF
                     VPAILQKSSLGIHGGISAKYISEKHLGLMVELNYAQRGWTEDFPAESGFSYNKTLNYL
                     EVPFMTHIYFGNNVRFIINAGPQLGFLLGSSDHMNEALSADIAAKREENPEAQVGMQY
                     ILKPRSFDYGLIGGVGMELKTGAGNVELEGRYYFGLGDIFENRKTKENFFFNRSAHRI
                     IEAKLSYFFKVK"
     gene            complement(4163..6154)
                     /locus_tag="PLI30_07580"
     CDS             complement(4163..6154)
                     /locus_tag="PLI30_07580"
                     /inference="COORDINATES: protein motif:HMM:NF013630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HOI79448.1"
                     /translation="MHTSLGMRKIVLVILFAAFFTAISGAQIPALEKITTNGQPFYKY
                     KVKSGEGLLAISRTFNVTVDEILKHNPTAGSGLKNGQELLIPVREGLTASTVSAPLTQ
                     SGQQPPLDQNHTFRHTIARGETLYGIAQMYNTSVDEIIRNNSGLTENIAEGQVIVVPQ
                     QRSLSSTRENYRYHTILPKETLYSVSRTYSLKPEDLIAANTGLSVETFQIGKTIRVPF
                     YQMQADFIPYEQQTQNVTHKVKRGETLYSISQAYDVPVESIEKANPILVAGLKTNMEL
                     IVPVKVSRLDENARTREIEANRLLSLSNETQKTDVIRVGLLLPFLDKSGGQHLRLQEY
                     YEGFLLAVEKMKKEGANIEVYVFEIGSRAKLESLLGTLEMESLHLLIGGMTDDQISLI
                     SNFSKKHNIKYIIPFSSKNSEVQNNNHVFQVNSPQSYFYSKASGVFMETFRNANVVIV
                     NAPGRSDKAEFLSTLKADLRRRNIRYQELSLTSNLSTDMLPLLNSNGENVIVPSTGDS
                     GSLREITAALTQVHQARQGVITRLFGYPEWQTYSTEMKKLFHQYGTYFYTSFYVDEQD
                     VETRQFAENFKLWYGRDLINTYPKYGMFGYDTGMFFLNAVYRYGTNFEQRINRIPSNS
                     LQFSFKFDRVNNWGGFINTGLYLIHYDAGNVVHKINKSR"
     gene            complement(6257..>6558)
                     /locus_tag="PLI30_07585"
     CDS             complement(6257..>6558)
                     /locus_tag="PLI30_07585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI79449.1"
                     /translation="EITMNKVQHFFSILILPAFVMFAVSCDSVNSDAKKAATFTNKSI
                     EKTSQLKLEEAEKLYRKSQIIIKKYESHRKGEKFSKLYQQYRDEGKINPQEQNRR"
BASE COUNT         1856 a         1462 c         1292 g         1948 t
ORIGIN      
        1 aaatccgtaa gtcacaaaca caaacggcac atcggcctct gaagaagctt tgctgtccga
       61 atcggtatca cctatataca aaggaaattg caggttattc cgttccttga gtagtttcat
      121 attaaacgac ttgggtttca ggttttctcc atgctccata taatcggtaa agaggtgtgc
      181 agttccggtg tgcttcataa agtttaccaa gcctcctttc tcacagttgc tgagcagaaa
      241 taacggatat tttttcgata atttctccaa tccctcataa acaccttcgt aaatatgcgg
      301 tttcataacg gggaccagca aatggtattg ccggacaacc tcgtcaaaca actcatcctg
      361 agccgctctt gacgcactgg ggagaacagc gtcgagcatg acacgcgctt cttttcccat
      421 aaggcccagt aggtcgtcac gggtaacccg tgaacgataa ccatttattt ccaatccctt
      481 attccagaca atgacgtaag aatcaacatt gtcccaaagg gttccgtcca tatcaaaaat
      541 taaactgtcg gggtttgtca tacattgaat agttgttatc cggcttcatc ttacctgcca
      601 gccggcaggc cgattcattt cagcagcaat agtcttaaca aaacaacggg tacctgttga
      661 ataaaggcat cctgtaagcc ggaaaacttt tttgtttgta agctgcaaaa gtactcaaca
      721 atgcccgaaa gggcaaataa ataatcatta aaataccgga gaaacagttt ttatcttatt
      781 tttgtacaaa atacaagata tggagaaaac ccgcgtttgc gtcataatca atccggtatc
      841 gggaacagaa tcgaagaaaa atatccctga agaagtggcc tccgcattcg atcaaaccca
      901 attcgatgta attatccgga tcaccggcta tcccgggcat gccactgaaa ttgccaaaca
      961 ggctgtgaag aacaaatttg cttatgtgct gactgtcgga ggcgacggca cggtaaatga
     1021 agtggcaaaa gccctggtga ataccgatac cattctggga atcattcctt ttggatcggg
     1081 gaacggactg gcacgcgacc tgcatatctc catagattca gaaaaagcca tacaaaccgt
     1141 actcgaaggc aatgtccgaa ccatcgatta cggctctgcc aacgggcata ttttcttttg
     1201 cacctgcggc gttggatttg atgcgtttat cagcgacaag tttgccgaag aaaaaaaacg
     1261 cggccccctg ggctacgtcc gcaacattgt ggaaggcgtg gtcgagttca ggtcggaaga
     1321 gtacgaaatc acgcacgacg aaggtacact taacgaacgg gcatttcttc ttacctgtgc
     1381 caatgcctcc caatacggga acgaagtata cattgcaccc ggtgccgata tggaagacgg
     1441 gaaaatgaat gtatctatac tgaagccgct taatcccaat gaaattccac aaaccacgct
     1501 gcaacttttt acccggaata tcgataaaaa cagcaagatg atctcgttag tcaccagcaa
     1561 cctcatcatt aaaagaaaaa aaccaggcat attgcacgtt gacggggaac cggtgaatac
     1621 cgaaaaggac attgaagtga aaatgtacca caagggatta aaggtacttg ccccaaagca
     1681 agttgaagaa aaaccggtga aagaaagaga aaatattttc acggctatca cccgttggat
     1741 gaatatataa tcgtgaattc aggcaaagcg ggcacactca tcccgagtgc acccgctttt
     1801 cattcataac aagttattaa aacaaaaata aatctcaagt gctattccgc aatttgtgcc
     1861 acaaaacttt cggcatcgtg atacgcacca ctcataacct cctccactac tccacctatt
     1921 tgagaagcaa acaaagccga gttcatgcgt gaaacatagg ttttcccatc ggctttttcg
     1981 tgtacagaaa tgcgacaggg catcatgttt ccataaattc tcaactcatc ggccgaaagc
     2041 atacggtaag catatttcgg attgcagagt tctatgactt ttacaggaag aacttcatac
     2101 ccgttcttat tcatcgtctc tttcagatcg tgggtgtgcg taacacgcca tcctgcttca
     2161 gcgatgatat tgttgagtct ttcaaccgtc tcgtcgaaag catacctgct tttactctcg
     2221 aaaaacattt tgtccatgat cccgggcttt taatttttga gtaaaacttc gtcgatagcc
     2281 tgcttaaaag catctttcgg taatgcaccc tgtgccattt gcggagcttc gttcatcggg
     2341 acgaaaagca acgtcgggat actgcggatt ccaaaatcgg cagccagttc ctgctctgct
     2401 tcggtatcca ctttataaat gtaaatctgt ccttcgtatt ctgcagccag ttgttccagt
     2461 ataggggcta ccattttaca cggaccacac caatcggcat aaaaatctat aatagcgggt
     2521 ttatcaccca ggtattccca tgtttgggaa tttttttcgt agtttgcaac tttcgttaga
     2581 aaatcagctt ttgttaaatg aattgtttta gtcatatttg ttttatttaa attagtttcc
     2641 tgttgagtta cagctgcctg tggcgatgat gttgatttgc cggtgttatt gctgcacgcg
     2701 gcaaacataa acgatgtcac aagtgcggcg ataataagtt taatcttcat attgttgttt
     2761 gttttgtata taatttcttt atttaatgtt ctttatctct ttgcctctac cttcttcttt
     2821 cttaaaaata tcaaaaatta gcagtcccaa aatggcaccg taaatcgtca ttctccacgg
     2881 atttgaagtg atgggacaac tgcccgtgcc gcagccaacg aaatagtaat acaggtaacc
     2941 tccgatggca ccaacaatga ctcctgcaac tttcaatcca tgttttgtga aaaattcctt
     3001 catatttatt ttttgtcaat tacaatctat gatacaactc aacagttgga taacccgatt
     3061 gtttctaatg gaataaaaac aattttttcc gctcctgcga caatttaaaa taccttttgc
     3121 acgcatgtcg gtcaggtgat acgaaagcag cgattgttca caatcaatag cttcctgcaa
     3181 ctcagatacc gatttttcct tctcgttaga caacaaggta atcacactta acctgatttc
     3241 atttgccaag gctttcagca tgtacgctgc ctcttcgaat tgcgctttat ccaccttttg
     3301 ttcctttatt tccagcatgc taaactccat tgttttttta tcaaatcaac atatgaacat
     3361 atattcatat atttttgttc aaaagtagtc catttttttt tattcaccaa ctaaatagca
     3421 aaaaaatatt tacttgattt ttacaggttg ctcccgactg tattgtacgt tacttcactt
     3481 taaagaaata ggaaagtttc gcctcgatga tgcgatgggc agaacggtta aaaaagaaat
     3541 tttctttcgt tttcctgttt tcgaaaatat cacccaaccc gaaataatat ctgccttcga
     3601 gttctacatt acccgctccc gttttcagtt ccattccaac accaccgata aggccataat
     3661 caaaactcct gggcttcaaa atgtattgca tgcctacttg tgcttcagga ttttcctctc
     3721 ttttagcggc aatatcggca gacagggcct cgttcatatg gtcagagctt cccaacagaa
     3781 agccaagttg tggtcccgcg ttgataataa accggacatt attgccgaag tagatatgtg
     3841 tcataaaagg aacctccagg taattcagtg ttttgttgta agaaaatcca ctttcggcgg
     3901 gaaaatcctc tgtccacccc cgctgggcgt agttcaattc gaccatcaat cccaggtgtt
     3961 tttccgaaat gtattttgcc gaaatgccac cgtgaatacc cagagaagat ttctgtaaaa
     4021 tagccggaac aaaatcaact gtcgacataa gtgctccccc tcccactccg atataaaact
     4081 ctttctcaaa atcatttttt tgtgcaaaaa cctgcacaga ggaaaggata aacaacaaaa
     4141 ttcccggcaa caaacgtttc atttagcggc ttttattgat tttgtgtaca acgtttcctg
     4201 catcgtaatg aataagatac aatccggtgt taatgaaccc tccccagtta ttaacccggt
     4261 caaatttgaa agaaaactgg agtgaattcg acggtatccg gtttattcgt tgttcgaaat
     4321 ttgttccata acgataaaca gcattcagga aaaacattcc ggtatcgtat ccaaacatac
     4381 cgtatttggg ataagtattg atcaggtcac ggccatacca caacttaaag ttttcagcga
     4441 attgccgggt ttccacatcc tgttcatcca cataaaacga tgtataaaaa taagttccgt
     4501 actggtgaaa caattttttc atctctgtac tgtatgtctg ccactccggg tatccgaaca
     4561 gccgcgttat aactccctgg cgagcctgat gtacttgtgt gagggcagcg gtaatttctc
     4621 gcaacgaacc cgaatctccg gtagagggaa caattacatt ttctccattg ctattaagta
     4681 acggcaacat gtccgttgaa aggtttgatg tgagggaaag ttcctgataa cgaatatttc
     4741 tccgcctcaa gtccgctttt agggtagaga gaaactcggc tttatcggat cgtccgggag
     4801 cattcacaat aactacgttg gcattcctga atgtttccat aaagactccg gatgccttgg
     4861 aataaaaata agactgaggc gaatttacct gaaatacatg gttgttgttc tgaacctcac
     4921 tgtttttcga agaaaaggga atgatgtatt tgatgttgtg ctttttagaa aaattggata
     4981 taaggctgat ctgatcatcc gtcatgcccc caatcaaaag atgaagtgat tccatttcca
     5041 gagttcccag taagctttct agctttgccc tgctgccgat ttcaaaaaca tacacttcga
     5101 tgttggcccc ctcttttttc attttctcca cagccaacag aaaaccttca taatactcct
     5161 gcaatcgtag gtgctgtcca cctgatttgt ccaggaaagg aaggagaagg cccaccctga
     5221 tcacgtctgt cttttgcgtt tcattgctca ggctgagtaa ccggtttgcc tcaatttctc
     5281 tcgtgcgggc attttcatcc aaccgggaaa ctttgacagg aacaattaac tccatatttg
     5341 ttttcagccc tgcaacaagt atgggatttg ctttttcaat tgattcaaca ggaacatcgt
     5401 aagcctgcga aatagaatag agtgtttctc ccctttttac tttatgcgtc acgttttgag
     5461 tctgttgctc atacggaata aaatctgcct gcatctgata gaaaggaacc cggatggttt
     5521 tccctatttg gaatgtttct acggacaagc cggtattggc ggcgatcagg tcttccggtt
     5581 ttaacgaata tgtccttgaa accgaataaa gcgtttcttt cgggagaatc gtatggtagc
     5641 ggtaattttc acgggtagac gaaagtgatc tttgctgcgg cacaacgata acctgtcctt
     5701 cggcaatatt ttctgtcagc cctgaattgt tacggataat ttcgtcaaca ctcgtgttgt
     5761 acatctgtgc aataccatac agggtttctc cccgggctat agtatgtcgg aaagtgtgat
     5821 tttggtcaag agggggttgc tggccggatt gggttagtgg agcggaaacg gttgaggccg
     5881 ttaagccctc tcgaaccgga atcagcagct cctggccatt tttcaatcct gaacctgctg
     5941 taggattatg ctttagaatt tcgtctaccg taacattgaa agtccgtgaa atggccagta
     6001 acccttcacc cgatttaact ttatacttat aaaagggttg cccgtttgta gtgatttttt
     6061 caagtgcagg tatttgggca ccactaatcg ccgtaaagaa ggcagcaaaa aggatgacca
     6121 acactatttt tctcattccg agtgacgtat gcattccgtt tgttttaaat tttccaataa
     6181 aaactagtaa agggcaaaat tacaaataat aattatttat gtgtcttcgc ttgtgttaat
     6241 taaagtgata acaactttat cggcgattct gttcttgagg attgattttt ccttcgtcac
     6301 ggtattgttg gtaaagttta ctgaattttt cccctttccg gtgcgactcg tactttttaa
     6361 tgattatctg tgattttctg tacagctttt ctgcttcttc caacttcaac tggctggtct
     6421 tttcaatgga tttattggta aaggtagccg ccttctttgc atcggaattc acactgtcac
     6481 aagaaaccgc aaacataaca aatgcaggca agataagtat tgaaaaaaaa tgttgaactt
     6541 tgttcattgt tatttcat
//
LOCUS       DAOSDR010000166         6558 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrimonas sp. isolate AMR_MDS_1826
            NODE_4248_length_6558_cov_2.424573, whole genome shotgun sequence.
ACCESSION   DAOSDR010000166 DAOSDR010000000
VERSION     DAOSDR010000166.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863355
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrimonas sp. (wastewater metagenome)
  ORGANISM  Petrimonas sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Dysgonomonadaceae; Petrimonas.
REFERENCE   1  (bases 1 to 6558)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6558)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:10:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,720
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 35
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6558
                     /organism="Petrimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4248_length_6558_cov_2.424573"
                     /isolate="AMR_MDS_1826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2023866"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(158..2545)
                     /locus_tag="PLI30_07590"
     CDS             complement(158..2545)
                     /locus_tag="PLI30_07590"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis tyrosine autokinase"
                     /protein_id="HOI79450.1"
                     /translation="MKNYSTVEEEYGMLAEKPIDVVGILMKYLSYWKWFLISLFLCLV
                     VAAVYLFYTLPQYKVETSILFKDDQRGGGASEMNVFKEMGVIRQKNNVDNEIEILKKS
                     LIVERVVRKLGVYASYTEIKPFKIIQLTGLDKFLPDFPKRKMRILYGDETPVLINIAE
                     NTLNNLEKNIVFDVWITPTGDYEFFGDYNDKKYRVKASSSDSIVAFPFGSLKLVKRNP
                     GPVESKFVRVKIEHPVNVAARYLNSLEIELTSKNSSVANLALTCPNGMLGKDFLEEYI
                     NTYNEEGIRDQIELADKTSQLIDDHLSKLSEELSSVETQVQDYKQSQGLTDIASQADV
                     YNTQSASVGQMKVEAETQYSIVSSLNNYVQGKRDHDQLIPANSGINSEALTAQINAYN
                     DLVLERNRLSRIASSSNQSMIDLNNRIESTFNSVKSGLQNEKNNLEIQQRDISAMYYR
                     NNARIRAIPRQERVYSDIKRQQNIKEELFLYLLQKKEEKYMNMASVEPNTKLVDNIRL
                     LGAVSPNRRMIGLLFFALGLVIPVACIKIKDLLRYQVSDKEELEELTPVPILGEIPQV
                     SHSEHIVIRENNNDNFSEMMRLLRANLLFVIDSKEKKVINMLSSISGDGKTFVTINMA
                     MSLALLDKKVLIVELDIRKPKLSDYLGIDNKKGITLYLSGNLNKEELVKPSGVHPNLS
                     VITAGSIPPNPNELLAKPLLDELISDLRRDFDFIIIDTAPVGVVSDSFLLNRLADVNL
                     YVIRADYTPKKFIEDAARYYKEDKLTRMYFVLNSVNMNAIAYRYGYGKKYGYGYA"
     gene            complement(2592..3389)
                     /locus_tag="PLI30_07595"
     CDS             complement(2592..3389)
                     /locus_tag="PLI30_07595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019541279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015159 - polysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015774 - polysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis/export family
                     protein"
                     /protein_id="HOI79451.1"
                     /translation="MKKNSFLLILIAGLTISSCSPVKDIAYFPQFQREITTVNESLYD
                     ARIKPKDLLSITVVSSEPEASRRYNLFTPQAQDLTNSLNSQPTIQSYLVDNEGNIELP
                     ILGILNVKGLNTKELESLIEKRLTPFFTGEMPIITIRILNYSINVLGEVLRPGKFVTA
                     NERITIFEGLALAGDMTIYGKRDNVKVLREGMNGERKIYTVNLNDKNVFDSPAYFLEQ
                     NDVVYVEPNQSRANSSRFGAAETYRISTLSVLISLATMAATIYSITR"
     assembly_gap    3862..3871
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            4302..5567
                     /locus_tag="PLI30_07600"
     CDS             4302..5567
                     /locus_tag="PLI30_07600"
                     /inference="COORDINATES: protein motif:HMM:NF016906.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HOI79452.1"
                     /translation="MKKNLLIVLNILFVCFTVHSSYGQGSRYTGSYIKSSTIQHVGKS
                     NFVIEGLEFTSNDRDLIVLYSCENVVIKNNSFVASPAKRGIYLDNCKNITIIDNTFDN
                     VHTGLVAHNSRGIKFEYNDVKNVGGTMKEEDKNNGFVALFDKVNGEGNSISYNVSENI
                     FGQSSPGDLINLNQSNGTAQSPIVVKGNWIRGGGPSPSGGGIILGDIGGSYQVAEDNI
                     VVDGGQYGISIAGGHDIALRNNKIYGSKNYFTNVGLYACNWYEENLGKSYNITVAGNV
                     VNYRNKEGNISNWWYASNVEPVSGKETNRYDPALSPSILPEQIIGRARMSVEPQPEPD
                     PDEKPDDIVLDPSISIYKDTFDRICANCKGSISSSASLSVLNQNGQKILSIPIIGYHT
                     TIDNPIPSGTYTVMVENDARLQIKKIVIN"
     gene            5590..6030
                     /locus_tag="PLI30_07605"
     CDS             5590..6030
                     /locus_tag="PLI30_07605"
                     /inference="COORDINATES: protein motif:HMM:NF024292.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-binding protein"
                     /protein_id="HOI79453.1"
                     /translation="MNKTIYIILLLVAFVVVSCERNEEKIELTSALNGEWEVVAYLGD
                     TPIHDGAFPLIISSDFPLKTDSITINDTSKYFWNFQVKVSADHENGTFETQKSICEHC
                     SYGIGIKVFNGKIIDSDSIYLEIQFEDDETPYGNTYQLKGYRVN"
     gene            6043..>6558
                     /locus_tag="PLI30_07610"
     CDS             6043..>6558
                     /locus_tag="PLI30_07610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008769762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HOI79454.1"
                     /translation="MEKLKVLINAYACSPDRGSEPGMGWNWCVHLANHCELYIISEGE
                     YRESIEAALATLPQAGNMHFYYNPIPEKVREMCRKQGDWRFYVHYKNWQKETLNIARK
                     IIAEHKIDVLHQLNMIGFREPGYLWKIEGIPFVWGPVGGLKQFPLAYLKDAGLKMRFF
                     IRLKNLINTLQI"
BASE COUNT         1882 a         1287 c         1380 g         1999 t
ORIGIN      
        1 ttttaagttc ttctatcacg gataaatttc ctcttctgtc caaatttttc ggaaggtcaa
       61 ttattttcgc accattgagt ttcattgttc tgcgtttttt aattcatttg tctgtctttt
      121 ttgttttaag tcaatgtttt atgctggtat ttctgcttta cgcgtatccg tatccgtatt
      181 ttttgccata tccgtaacgg taggcgatgg cattcatgtt tactgagttc aggacgaaat
      241 acattcgggt aagcttgtcc tctttgtaat atcttgccgc gtcttcaatg aatttcttgg
      301 gcgtatagtc cgctcgaata acataaagat tcacatcggc aagacgattc agtagaaaac
      361 tgtcggaaac cactcctact ggcgccgtat caataattat aaaatcaaaa tctcttcgca
      421 gatcgcttat cagttcatcc aacagaggtt tcgctaataa ttcgttggga ttaggcggga
      481 tggacccggc ggtaatgacc gacagattgg gatgaactcc tgagggtttc accagttctt
      541 ctttgttcag gtttcccgac aaatagaggg taatcccctt tttattgtct attcccaggt
      601 aatctgacag cttgggctta cgaatatcca gctcaacgat caacactttt ttatcgagca
      661 gggctaagct catggccata ttgatggtaa cgaatgtttt cccgtctcca ctgatgctcg
      721 acagcatatt gattaccttt ttttctttac tgtcgataac gaataatagg ttggctctta
      781 gtagcctcat catttcgctg aaattatcat tgttgttttc ccggatgaca atatgttcac
      841 tgtgagaaac ctggggaatt tcacccagga taggaaccgg agtcagttct tccagttctt
      901 ccttgtcgct aacctgatat cgcaacaggt cctttatttt aatgcaggca acaggtataa
      961 ccaatcccag ggcgaaaaac aacaatccga tcattcttcg gtttggtgat acggcaccca
     1021 ggaggcgaat attatccacc agtttggtgt tgggctcgac cgaagccata ttcatgtatt
     1081 tttcctcttt tttttgaaga agatacaaaa acaattcttc cttgatgttc tgctggcgtt
     1141 ttatgtcgga gtaaaccctt tcctggcggg ggatagcccg tatccgggcg ttattccggt
     1201 aatacatggc ggaaatgtcg cgttgctgaa tttccaggtt gttcttctcg ttttgcaagc
     1261 cagatttcac ggaattgaaa gtggattcta tccggttatt caagtcgatc atggactggt
     1321 tactgctcga agcaatgcgc gagagcctgt ttcgctccaa aaccaaatca ttgtaggcat
     1381 tgatctgagc tgtcaaggct tcactgttta tcccggagtt tgcggggata agttgatcgt
     1441 ggtctctttt tccttgaaca taattattca ggcttgaaac aatcgaatat tgagtctctg
     1501 cctccacttt catttgtccc acactggcag attgcgtgtt gtagacatct gcctgagagg
     1561 caatatcggt cagtccttgc gactgcttgt agtcttgtac ctgcgtctcc acagaactga
     1621 gttcctcgct taatttagag agatggtcat cgataagttg ggatgttttg tcggctagct
     1681 caatctgatc ccttatgcct tcttcgttat atgtattgat gtattcttcc aaaaaatcct
     1741 ttcccagcat tccattcggg caagtcaacg ccaagtttgc cactgaagaa tttttggaag
     1801 tcagttctat ttccagggaa ttcaggtaac gggctgcaac gttcacggga tgttcaattt
     1861 ttacccttac gaacttgctt tctacgggac ctgggttacg cttaaccaat tttaaactgc
     1921 caaacgggaa agccactata ctgtcggacg atgaagcttt tacacggtac tttttatcgt
     1981 tgtaatcgcc aaagaattca taatcgccgg tgggcgttat ccatacgtca aaaacgatgt
     2041 ttttttccag gttattgagt gtgttttcag ctatgttaat aagaacaggg gtctcatccc
     2101 cgtatagaat tctcattttt cgttttggaa aatccgggag gaatttgtcc agtcccgtca
     2161 gttgaataat cttaaacggt tttatttctg tgtatgaggc gtaaaccccg agcttcctca
     2221 ctacgcgctc aacgatcaat gatttcttta agatttcaat ctcattatcc acgttgttct
     2281 tttgccggat gactcccatt tccttaaaca cattcatttc agaagctccc cctccccgtt
     2341 gatcgtcttt gaacagaatt gaagtttcta ccttgtattg cggcaacgtg taaaaaagat
     2401 agacagcggc aacaaccaga cagaggaaca gggaaattaa aaaccatttc cagtaagaga
     2461 ggtatttcat cagtataccc acgacatcaa ttggtttttc agccagcata ccgtattctt
     2521 cttcaactgt ggagtagttc ttcattgttt atgttttgtt tataaagtct ttatacaggt
     2581 aaggcctttc attaccgggt gatgctgtag attgtggctg ccatagtggc taacgagatc
     2641 aggacagaca acgttgaaat ccggtaagtt tccgccgcgc caaatcggga agagtttgcc
     2701 cgggactgat taggctctac atataccaca tcgttttgtt cgaggaaata agcgggagaa
     2761 tcgaaaacat ttttatcgtt gaggtttacg gtgtaaattt tcctttcccc gttcattccc
     2821 tcgcgcaata cttttacatt atcacgcttg ccgtaaatag tcatatcgcc tgccaaagcc
     2881 aatccttcaa aaatagttat ccgttcgtta gcggttacaa attttcccgg acgaaggacc
     2941 tcacccaaaa cgtttatcga ataatttaaa atgcgaatgg ttataatggg catttctccc
     3001 gtaaaaaacg gggttaaccg cttctcaatg agggattcca gttccttcgt gtttaatcct
     3061 ttcacattca ggattcccag tatgggaagt tctatattcc cttcgttatc aactaggtag
     3121 ctttgaatgg tgggttgtga attcagcgag ttggtaaggt cctgtgcttg gggagtaaaa
     3181 agattgtatc ttcgcgaagc ttcaggctca ctgctgacta cggtaattga gagtaaatct
     3241 ttgggcttta tgcgggcatc gtacaacgat tcattgacgg tagttatttc tctctggaat
     3301 tgagggaaat aggctatgtc ttttaccggc gagcaggaag agatagttaa ccccgctatg
     3361 aggataagca agaaactgtt ttttttcata ctgtgatttt taattgagct gtatcgtctc
     3421 tgttggcatc attaagcaga ttcctaccgt tttgtagtta tccgacgtaa tttttctaag
     3481 attttcagaa cgactttctg ctttaagcag acctttttat gctaaacatt aagtttgtgc
     3541 aacaattttg tgacaacttg tttacactgc tgacaaaatt aagaaagaaa ggaaggggta
     3601 tgttaggact atatgaacat ttttttgtat ttccggatta attttattgt taaattataa
     3661 ctacacgggc atattgaatt tacaatccat taagaatgag atttatattt ttaaaaaagc
     3721 ttttacccgc caaatttaaa aaacaggaaa gagagaaccg aggaacacgc tgcgtgaaaa
     3781 atttataatt ataggataaa atacatataa tcaattatat atatattcaa caacctgaat
     3841 tcatcatttg ccatccattt tnnnnnnnnn nttttttatc taatttagtt aattgtcggg
     3901 cagcttcatg ctgtaattgt cctaattcgt ttcatttttg tctacaagaa aattcgttga
     3961 aaccggaaat ttaccttcaa aacgccacca gaaaaccatc cctcaaaaaa gtgtttatac
     4021 cgattctaaa caggtaaaaa taattggtaa aatatgcgca ttgttaatta agggcaacag
     4081 cttttaattt tgcctaaata gtgttatttg gttgtaattt agtactataa taagaaattt
     4141 gtacgtaatt ttgcagcgta ataaatcatt tgatacaatt ctttgttctt catgcatata
     4201 ttttcctatt ttaaagatac aacaatccat tctttttgta aattgcacag gaaaagcagg
     4261 ctgcctcagt gaattgtatg aataaaatca aaaagcattc aatgaaaaaa aatctactca
     4321 tcgtactgaa tatactgttc gtatgcttta ccgttcattc gtcttatgga caaggttcca
     4381 ggtataccgg ttcttacatt aaatcctcca cgatacagca cgtagggaaa agcaattttg
     4441 taatagaggg attggaattc acaagcaacg atagggacct tattgttttg tacagttgcg
     4501 aaaatgtggt aattaaaaac aatagttttg tggcatctcc cgcaaagaga ggtatatacc
     4561 tggataactg taagaacatt acaattattg acaacacctt tgataacgtg cataccggat
     4621 tagtagcaca caattcccgc ggaataaaat ttgaatacaa cgacgtgaaa aatgttggcg
     4681 gcactatgaa agaagaggac aaaaacaacg ggtttgtcgc gttgttcgat aaggtaaacg
     4741 gcgaagggaa tagtataagt tacaatgtat cggagaatat tttcgggcaa agttcgcccg
     4801 gtgaccttat taacctgaat cagtcaaacg gtacggcgca gagcccgatt gtagtgaaag
     4861 ggaactggat cagggggggc ggcccgtctc catcgggagg aggtattatt ttaggcgata
     4921 ttggcggttc ttatcaggtg gctgaagaca atattgttgt ggacggagga cagtacggaa
     4981 tctctattgc aggcggccac gatatagctt taagaaacaa caagatatac ggcagcaaaa
     5041 attacttcac gaacgtagga ctttatgcct gtaactggta tgaagaaaat ttaggcaagt
     5101 catataacat caccgtagca ggtaatgttg tcaattacag gaacaaagaa ggcaacataa
     5161 gtaattggtg gtatgcttcc aacgtggagc cggtaagcgg taaggaaacc aacaggtatg
     5221 accccgccct atctccgtcg atactccccg aacagatcat cggacgggca aggatgtcgg
     5281 tagaaccgca gccggaacca gatccggatg aaaagcccga tgacatagtg ttggatcctt
     5341 cgataagtat ttataaagat acatttgacc gtatttgcgc caattgtaaa ggctctatat
     5401 cttcttctgc atcactatcg gtcttaaacc aaaacggaca aaaaatactc agcataccta
     5461 taatcggtta tcacactact attgacaacc caatcccctc gggaacgtat accgtgatgg
     5521 ttgaaaacga tgcccgcttg caaatcaaaa aaattgtgat taactaattt agctagagaa
     5581 cgagagaaaa tgaataaaac aatatatatt attttactgc tagtagcgtt tgttgtcgta
     5641 tcctgtgaac ggaacgaaga aaaaatagaa ttaaccagtg ccttaaacgg agagtgggag
     5701 gtcgtggctt acctgggtga tactcctatc cacgatggag cgtttccgtt aattatttcc
     5761 agtgactttc cattgaaaac ggattccatc acgattaacg atacatcaaa atatttttgg
     5821 aattttcagg ttaaggtttc agctgatcac gagaacggaa cctttgaaac gcaaaaatct
     5881 atttgtgaac actgcagcta cgggattggt ataaaggtat ttaacggaaa aataattgat
     5941 tctgacagca tttatctgga gatacagttt gaagacgatg aaacacctta tggcaataca
     6001 taccagctaa aagggtacag ggtaaactaa gtcagccgga aaatggaaaa gctaaaagta
     6061 ctcattaatg cttacgcctg ttcaccggat aggggaagtg agcctgggat ggggtggaac
     6121 tggtgcgtac acctggccaa ccattgtgaa ttatatatca tcagcgaagg ggagtaccgg
     6181 gagagtatag aagctgctct ggcaactttg ccacaggcgg gaaatatgca tttttactac
     6241 aatccaatac cggagaaggt gagggagatg tgtagaaagc agggcgactg gaggttttat
     6301 gtccattata aaaactggca aaaagagaca ctcaacatag cccggaagat tattgccgaa
     6361 cacaagatag atgttttgca tcaattgaac atgattggtt tccgggagcc aggctacttg
     6421 tggaaaatag aaggtattcc ttttgtatgg gggcctgtag gcggattgaa acaatttccc
     6481 cttgcctacc tgaaagatgc cggactcaag atgcgctttt ttattcgttt gaaaaacctc
     6541 atcaatacct tgcagatc
//
LOCUS       DAOSDR010000167         6547 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrimonas sp. isolate AMR_MDS_1826
            NODE_4254_length_6547_cov_3.528805, whole genome shotgun sequence.
ACCESSION   DAOSDR010000167 DAOSDR010000000
VERSION     DAOSDR010000167.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863355
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrimonas sp. (wastewater metagenome)
  ORGANISM  Petrimonas sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Dysgonomonadaceae; Petrimonas.
REFERENCE   1  (bases 1 to 6547)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6547)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:10:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,720
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 35
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6547
                     /organism="Petrimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4254_length_6547_cov_3.528805"
                     /isolate="AMR_MDS_1826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2023866"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1005)
                     /gene="rmuC"
                     /locus_tag="PLI30_07615"
     CDS             complement(<1..1005)
                     /gene="rmuC"
                     /locus_tag="PLI30_07615"
                     /inference="COORDINATES: protein motif:HMM:NF014680.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HOI79455.1"
                     /translation="MEITGLLIGFVAGGIIAWIMATLLVRLKTVSKQELEAVNEKNVS
                     LHTDLIVAQEKLKSMQEDEAELRTEIAAYREKTEQSRDEVQALERNLSGTTAQLNVAH
                     DTVQKQLEEIGTYKLELKSMTDEFNETNRLLAASTADFNALKEKLETQKSEMENLRKQ
                     FNLEFENIAEKILDEKTHKFTKINQENLDIILKPLGENIDSFRKKVEEVYITEAKERF
                     SLGEEVKKLKELNTKLSEDAVNLTNALKGSSKTQGDWGQMILENILEKSGLVRDREYF
                     VQEFLKDVDGAYLVNEDGSKMQPDVIISYPDDRKVIIDSKVSLSAYVRYTQTDEVDEQ
                     K"
     gene            complement(1022..2242)
                     /locus_tag="PLI30_07620"
     CDS             complement(1022..2242)
                     /locus_tag="PLI30_07620"
                     /EC_number="3.5.4.25"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004332943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA];
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3,4-dihydroxy-2-butanone-4-phosphate synthase/GTP
                     cyclohydrolase II"
                     /protein_id="HOI79456.1"
                     /translation="MKEFTLNTIEEAIEEIKKGNFIIVVDDEDRENEGDLIIAAECIT
                     PEKVNFMETHARGLICTPITKERAEELELPMMVTHNTSIHSTPFTVSIDLLTHGCTTG
                     ISAYDRAQTILALAREETKPEDFGRPGHIFPLRAMSKGVIRRAGHTEATIDLARLAGL
                     YPAGALAEIKKEDGEMARMPELMKMAKEFNLKIISVADLIKYRLRTESLVNRGEEVHL
                     PTQYGDFHMVPFRQKSNGLDHVALIKGEWHKDEPILVRVHSSCITGDIFGSMRCECGE
                     QLHKAMQMIEKEGKGVLVYLNQEGRGIGLMAKASAYKLQEKGMDTVDANLHLGYKADE
                     RDYGVGAQILQSLGVTKMRLMTNNPVKRIGLESYGLEVVENVPIEITPNEYNHFYMET
                     KKKKMGHILKNSGL"
     gene            complement(2253..4193)
                     /locus_tag="PLI30_07625"
     CDS             complement(2253..4193)
                     /locus_tag="PLI30_07625"
                     /inference="COORDINATES: protein motif:HMM:NF015684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LptF/LptG family permease"
                     /protein_id="HOI79457.1"
                     /translation="MRRDFHIKKLYGYLLKTYIPLFLMTFAICLFLVLMQFLWKYVED
                     MVGKGLGLNVIGEMFLYAALQLIPLALPLSILLASLMMFGNLGENLELLAIKSAGISL
                     IKAMVPLIALIVLICIGTFFFQNNAMPKIQPKFYSLLISIRQKSPELDVPEGVFYKEI
                     EGYNLYVDKKNRKTGMLYGVSIYDVSSGFQNMAVIICDSAEMRTSEDKLSLVFTMHSG
                     QQFQNFTQGTENMNFSSEFIPYARESFVTKTMTIPYDDNFNRMEESVLQESESSNYVS
                     KNIAQLRVSIDSLEQFIDSVNISDRKIMKQYTYLSFRNSYPQEKRDSIVEHATASMKP
                     DMDSIFASKDIQTQTAILQNAYSKADNNGNDYLFRSMSKTTTQRNINRQWIEWHRKFT
                     IPFACLVFFFIGAPLGSIVRKGGLGTPIVISVILFIIYYILDNVGYKMARDGVWDHWI
                     GMWFSSMILLPLGIFLTYKAMTDSVIMSTDTYTSFFKKLFFIREKRNYTIKEVVIEEP
                     NYAEVYQSSRELTDEVAAHLQKYGTLGYKTYWTDNGYDHSLTSIKNKMENMLNQLSNS
                     RKPMVIEKAKEYPVLIKYVRPFNTGSTLAKLCMYIFPLGVIMKLLSLPFEKRINRDLN
                     AVLTLNNELNEIMNNQQRKVAV"
     gene            complement(4335..4724)
                     /locus_tag="PLI30_07630"
     CDS             complement(4335..4724)
                     /locus_tag="PLI30_07630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005867543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="START-like domain-containing protein"
                     /protein_id="HOI79458.1"
                     /translation="MLKEKFHIEFVMGSATQASLWRMISQVDGLSEWFADEVTMNEEE
                     NVYTFFWGKSDNQAEILGSKPQQSIRFRWLDEEEENIYFEFQLHKLELSNEIALQITD
                     FAEPDEKGDAITLWETQIDKMKRRLGV"
     gene            complement(4836..5588)
                     /locus_tag="PLI30_07635"
     CDS             complement(4836..5588)
                     /locus_tag="PLI30_07635"
                     /inference="COORDINATES: protein motif:HMM:NF013866.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HOI79459.1"
                     /translation="MTTKHFFLIAAVFSFSISLAQGKFVTYEVDNEPYEGYYIRPSTG
                     NPGNAALVLILHDWDGLTDYEVKRANMLAEEGYQVFAADLFGKGIRPTENKEKAQHTG
                     ELYKDRSKLRRLMTGALHAARQQGAETEKVAAMGYCFGGAAALELARSETALKGFATF
                     HGGLGTPEGQDYSKTRGKIIIFHGTADTAITMDDFAALAKELEAAKIPHEITSYGNAP
                     HGFTVFGTPAYRLEADKQSWKRFIKFLEEIFS"
     gene            complement(5682..>6547)
                     /locus_tag="PLI30_07640"
     CDS             complement(5682..>6547)
                     /locus_tag="PLI30_07640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI79460.1"
                     /translation="NVVIILCFFSQLVSAQQLAQNHLSNLPKFPDNGCAVTETNKNTF
                     YQQIDRVIEAIELDIERIHKQQPPADQNQLLSTVSGGEAAGEQLAQQKLMKDHGISSM
                     DLKKIENMSEEELQEWGEKYVASNRGKHSQSSARKQYNIKLHDLQDRLSTYKEGWVNM
                     QAEYEKKEKQAKTDLDLCLEMVLKNAPEPQYRGEACINQDVIDEYITKNEQRCNDNYC
                     QAIAPLKKQMMNKKMGEMPQVLRLLSQIQTIQNDQVKEQTGLNLNASQSNELAALELV
                     KEFAGEMRNML"
BASE COUNT         1519 a         1515 c         1445 g         2068 t
ORIGIN      
        1 tttttgttcg tcgacttcgt cggtttgtgt gtaacgaaca tacgccgaca gcgatacctt
       61 ggagtcgata attaccttgc ggtcgtcggg gtaggagatg atcacatcgg gttgcatttt
      121 gcttccgtcc tcgttaacga ggtaggcacc gtcgacgtct ttcaggaact cctggacaaa
      181 gtattccctg tcgcgcacca atcccgattt ctcgaggatg ttctccagga tcatttgtcc
      241 ccaatcgcct tgtgttttgg agcttccttt caaggcattg gtcaggttca cggcatcctc
      301 gctgagtttt gtattgagct ccttcagctt ttttacttct tcgcccagtg aaaacctctc
      361 tttggcttcg gtaatgtaga cctcttccac ttttttccgg aacgagtcga tattttcacc
      421 taacggcttc aggatgatgt ctaagttctc ctgattgatc ttggtgaact tgtgcgtttt
      481 ttcgtccagg attttttccg cgatattttc aaactccagg ttgaattgtt ttcgcaggtt
      541 ctccatttcg gacttttgcg tctccagctt ctctttcagg gcgttgaagt cggctgttga
      601 ggcggcaagc aacctgttgg tttcgttaaa ctcatcggtc atcgatttta attccagctt
      661 gtaagtgccg atttcttcca actgcttttg tacggtgtcg tgggctacat tgagctgggc
      721 agtagtgccg gagaggttac gttcaagggc ctgaacttcg tcccgggact gttctgtctt
      781 ttcccggtat gccgcaatct ccgttcttaa ttccgcctca tcttcctgca tggacttcag
      841 tttctcctgc gctacaatga ggtcggtatg tagagagaca ttcttctcat tcacggcttc
      901 aagttcttgt tttgataccg ttttcaacct gacaaggagg gttgccatga tccaggcaat
      961 aattcctccg gccacaaaac ctatgagaag accggttatt tccatttttt tgcttttacg
     1021 gttacaatcc ggaatttttc aggatatgtc ccatcttttt cttcttggtt tccatgtaga
     1081 aatggttgta ctcgttgggc gtaatttcga taggcacatt ttccaccact tccagtccgt
     1141 aagattccag tccgatacgt ttcacgggat tgttggtcat taaccgcatt tttgtgacgc
     1201 ccaggctttg caggatttgt gcacccacgc cgtaatcgcg ctcgtctgcc ttatagccta
     1261 aatggaggtt ggcatcaacc gtatccattc ccttttcctg taatttgtag gccgaagcct
     1321 ttgccataag tccgattcca cgtccttcct gattgagata aaccaacacg ccttttcctt
     1381 ctttttcgat catctgcatg gctttatgca gttgttctcc gcattcgcat cgcatggagc
     1441 cgaaaatatc gccggttata cacgacgagt gaacgcgcac caaaatgggc tcgtctttgt
     1501 gccattctcc ttttatcagt gctacgtgat ccaatccgtt ggacttctgg cgaaaaggaa
     1561 ccatatggaa atcgccgtac tgggtgggta aatgtacctc ctccccgcgg tttacgagcg
     1621 actctgtcct caggcgatac ttgatgagat cggcaaccga aataatcttc aggttaaatt
     1681 cctttgccat tttcatcagt tcgggcatgc gtgccatttc cccgtcttcc ttttttatct
     1741 ctgccagcgc tccggcaggg tacaatccgg ccaaacgggc caggtcaata gtagcttccg
     1801 tgtgtcctgc acggcgaata acgcctttgc tcatggctcg taacgggaaa atgtgtcccg
     1861 gccggccaaa atcttcgggc ttggtttctt cacgtgccag cgccagtatg gtttgtgccc
     1921 ggtcgtaggc cgagatgccg gtcgtgcagc cgtgagtgag taaatctata gagacggtaa
     1981 acggtgtgga gtggatggag gtgttgtggg tgaccatcat gggcaactcc aattcttcgg
     2041 cacgttcttt ggtgatgggt gtacagataa gtccccgggc gtgcgtctcc ataaagttga
     2101 ctttttccgg cgtgatacac tcggcagcaa tgatcagatc gccttcattt tcccggtctt
     2161 cgtcgtcgac cacgattatg aagtttcctt tttttatttc ttcgatagct tcttctatcg
     2221 tgtttagcgt aaattctttc attttgtatt gtttatacag ctactttacg ttgctggttg
     2281 ttcattatct catttaattc gttgtttaac gttaatacgg cgtttaagtc cctgttgatt
     2341 cgcttttcaa aaggcagtga aagcagtttc attataaccc cgagagggaa aatatacatg
     2401 cacagtttgg caagtgtgga tcccgtatta aaggggcgta catattttat caaaaccggg
     2461 tattcctttg ctttctcaat aaccatcggc ttacgtgagt tggatagctg attgagcata
     2521 ttctccatct tgttttttat cgaggtcaag gaatggtcgt atccgttgtc tgtccaatag
     2581 gttttgtatc cgagggtgcc gtatttttgc aggtgggcgg ccacctcatc ggtcagctca
     2641 cgtgacgatt gataaacctc ggcgtagttg ggttcttcaa taacgacctc ttttatcgta
     2701 taatttcttt tctcgcggat aaagaaaagt ttcttgaaga agcttgtata ggtatcggta
     2761 ctcataatta ccgagtctgt catggccttg tacgtcagga aaatgcccaa cggcagcaga
     2821 atcatcgagc tgaaccacat gccgatccaa tgatcccaaa ccccgtcgcg ggccattttg
     2881 taacctacat tgtctaagat atagtagata ataaataaaa taaccgatat gacaatgggt
     2941 gttcccaggc cacctttgcg tacgatggag cccagtgggg caccgatgaa aaagaatacg
     3001 agacaggcaa aggggatggt gaattttcgg tgccactcga tccattgccg gtttatattt
     3061 cgttgtgtgg ttgttttgga catcgaccgg aaaagataat cgttcccgtt attgtcggct
     3121 tttgagtagg cgttttgcag gatggccgtc tgggtttgta tgtcttttga agcgaaaata
     3181 gaatccatat cgggtttcat ggatgcggtt gcgtgctcaa cgatggagtc ccttttctct
     3241 tgcggatagc tgttccggaa agaaagatag gtatattgtt tcattatctt ccggtcggaa
     3301 atgtttacgc tgtcgatgaa ttgctccaaa gagtcgatcg aaacccttag ctgtgcgata
     3361 tttttggaaa cgtaattgct cgattcgctc tcctgtaaaa cactttcttc cattcggttg
     3421 aagttgtcat catagggtat agtcatcgtt tttgtaacaa aactctcgcg ggcatagggg
     3481 ataaactccg aagaaaaatt catattttcc gttccctggg taaagttttg aaactgttgc
     3541 ccgctatgca tcgtaaagac aagcgataat ttatcttccg aggtccgcat ctcggccgaa
     3601 tcacagataa tgacagccat attctggaac ccgctgctga cgtcgtaaat ggaaacgcca
     3661 tacagcattc cggttttccg gtttttcttg tccacgtaca aattgtaacc ttctatctcc
     3721 ttgtaaaaaa caccttcggg aacatccagt tcaggtgatt tctgccggat ggagatcagg
     3781 agtgaataaa acttgggctg gattttcggc atggcgttgt tttgaaaaaa aaaggtgcct
     3841 atgcagatta agacaatcag ggcaatgagc ggaaccatcg ccttgatgag agagatcccc
     3901 gccgatttta tggccagcag ctccaggttt tcacccaggt tcccaaacat catcagcgaa
     3961 gccagcaata tggataaagg cagtgccaaa ggtatcaatt gaagcgctgc gtataaaaac
     4021 atttcaccta ttacgttcaa ccctagccct ttacctacca tgtcctcaac gtatttccat
     4081 aagaactgca tcaacaccaa aaaaaggcat atggcaaacg tcatcagaaa cagcgggata
     4141 taggttttta acaaatagcc gtaaagtttt tttatatgga aatcccttct catctgttaa
     4201 aagaagccct ttttaaattc gatacaaaga taataaattc ctgtggagaa aagtgttttg
     4261 aggtttaaga aaacaaaaag aacaaccggc ttgttttcgg ttgttctttt tgtttatata
     4321 gcgagggata gctactacac acccagcctt cttttcattt tatcgatctg tgtttcccac
     4381 aaggtaatgg catcgccttt ttcgtcgggt tctgcgaaat cggtgatttg taaagcaatt
     4441 tcattggata attccagttt atgaagctgg aactcaaaat atatattttc ttcctcctcg
     4501 tccaaccacc ggaaacgaat actttgctgg ggtttcgaac ctaagatctc tgcctgatta
     4561 tccgactttc cccaaaaaaa tgtgtaaaca ttttcttcct cgttcatcgt aacttcatcg
     4621 gcaaaccatt ccgataatcc gtcaacctgg ctgatcattc tccacaaact cgcttgtgtg
     4681 gcactcccca tcacaaactc aatatgaaac ttctctttaa gcataactgc tcacaataaa
     4741 ttgcgctcaa tatacaactt tttttccata taacggcatt tcacaacagt tttttggccg
     4801 ttgtataaaa atagatgagc agaattggct tgtttttaag agaatatttc ttcgagaaat
     4861 tttatgaatc gtttccaaga ttgcttatca gcttccaggc gatatgccgg agtcccgaac
     4921 accgtaaaac cgtgcggggc atttccgtat gaggttattt cgtgggggat ttttgccgct
     4981 tccaactctt tggcaagagc ggcaaaatca tccatcgtga tggcggtatc ggctgttccg
     5041 tgaaaaatga tgatcttccc cctggttttt gagtaatctt gcccttcggg ggttcccagg
     5101 ccaccgtgga aggttgcaaa acctttcaac gctgtttccg agcgggctag ctccagggcg
     5161 gctgcacccc cgaaacaata acccatcgcc gccacttttt ccgtttcagc gccctgttgt
     5221 cttgcagcat gcaaagcacc cgtcatcagg cggcgcagtt tactgcggtc tttatacaat
     5281 tctcccgtgt gttgggcttt ctctttgttc tcggtgggac ggatcccttt gccaaaaaga
     5341 tcggctgcaa atacctgata gccttcttcg gccaacatgt tggcacgttt tacttcgtaa
     5401 tcggtcagcc cgtcccagtc gtgaagaatc agtaccagtg cggcgtttcc cggatttccc
     5461 gtcgatggcc tgatgtaata gccttcataa ggctcattgt ccacctcata ggttacaaat
     5521 ttgccttgtg ccagtgagat ggaaaaagag aaaacggcgg cgatcagaaa gaaatgtttt
     5581 gttgtcataa tgctgtaatt tttatgttta cgcttataac acaaaatatt tacaaattgt
     5641 tgtctctatt cgcaataagg atgaggcacg tttgtttcat atcaaagcat atttcgcatt
     5701 tctccggcga attcctttac cagttccaac gcagccagtt catttgattg agatgcatta
     5761 aggttaagac cggtttgttc tttcacctga tcgttctgta tcgtttgaat ttgggaaagc
     5821 aacctcagta cctgaggcat ctcccccatt tttttgttca tcatttgttt cttgagtggc
     5881 gcgatagcct ggcagtagtt gtcattacaa cgttgttcgt tcttggtgat atactcatca
     5941 ataacatcct ggttgatgca tgcttcacct ctgtattgag gttccggggc atttttcaat
     6001 accatttcca aacacaggtc gagatcggtt ttagcctgct tttctttctt ttcatattca
     6061 gcctgcatgt tcacccaccc ctctttatac gtcgacagtc tgtcctgaag gtcatgtaat
     6121 ttgatgttgt actgcttcct ggcagatgat tgcgaatgtt ttcccctgtt ggaagcgaca
     6181 tacttctcac cccactcttg aagttcctct tcactcatgt tttcaatttt tttcagatcc
     6241 atcgaagaga ttccgtgatc tttcatgagt ttttgttgtg ccagttgttc gccggcagct
     6301 tcgccacctg ataccgtact taagagctga ttttggtcgg caggcggctg ttgtttgtgt
     6361 atccgctcaa tatccaattc gatcgcttcg atgaccctgt caatctgctg gtaaaaggtg
     6421 tttttatttg tttcggttac cgcacaacca ttgtctggaa actttggaag gttggagaga
     6481 tggttttgag cgagttgctg cgctgagaca agttgagaaa aaaaacacag aattataact
     6541 acattat
//
LOCUS       DAOSDR010000168         6525 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrimonas sp. isolate AMR_MDS_1826
            NODE_4271_length_6525_cov_3.838640, whole genome shotgun sequence.
ACCESSION   DAOSDR010000168 DAOSDR010000000
VERSION     DAOSDR010000168.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863355
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrimonas sp. (wastewater metagenome)
  ORGANISM  Petrimonas sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Dysgonomonadaceae; Petrimonas.
REFERENCE   1  (bases 1 to 6525)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6525)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:10:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,720
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 35
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6525
                     /organism="Petrimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4271_length_6525_cov_3.838640"
                     /isolate="AMR_MDS_1826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2023866"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..860)
                     /locus_tag="PLI30_07645"
     CDS             complement(<1..860)
                     /locus_tag="PLI30_07645"
                     /EC_number="5.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011107447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylose isomerase"
                     /protein_id="HOI79461.1"
                     /translation="METKKEFFPGIGKIRFEGKESRNPLAFRYYDAEKVVYGRKMKDW
                     FKFSMAYWHTLCAESSDPFGEETKDFEWNNGNGALERARKKLDAGFEFMQKIGIEYFC
                     FHDIDLIDEGKTIREYEANLRSIVAYAKKRMEETGIKLLWGTANVFSHKRYMNGAATN
                     PNFDSVAYAATQIKNALDATIELGGENYVFWGGREGYMSLLNTDMKREKEHLAMMLAK
                     ARDYARSKGFKGTFLIEPKPMEPTKHQYDTDAETVIGFLRAHNLDKDFKLNIEVNHAT
                     LAGHTFEHDL"
     gene            complement(884..2362)
                     /locus_tag="PLI30_07650"
     CDS             complement(884..2362)
                     /locus_tag="PLI30_07650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006801272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY family carbohydrate kinase"
                     /protein_id="HOI79462.1"
                     /translation="MYLLGYDIGSSSVKACLVNAETGQVVASDFFPKTEMPIYAAQPG
                     WAEQDPDMWWENLKLANQSVIKKSGIDVAEIKGIGISWQMHGLVAIDKNGKVLRPSII
                     WCDSRAVPYGEKAFEAIGAKNALAALLNSPGNFTASKLAWVKENEPCLYGQIDKIMLP
                     GDYIAMKLTGGVCTTIEGLSEGVFWDFQRNTVSTEVMSYFGFSNDIIPPTVPTFGFQG
                     TVCAKAAIELGLKEGTPLGYRAGDQPNNAVSLNVFNPGEIASTAGTSGVVYGVLGEVN
                     YDPLSRVNTFAHVNHTSGKPRLGVLLCINGTGILNSWIKRNMIQPGLDYNNMNELAQK
                     SPVGSKGVSVIPFGNGAERVLENKNPGCSFHGINFNIHTLSDIVRASQEGIVFSFRYG
                     MEIMEEMGMEINVIRAGNANMFLSPVFRETLAGVTGATIELFDTDGAAGAAKAAGMGV
                     GIYSSNEEAFASLKKIMTIGSDERDKERYEEAYQRWKTYITQ"
     gene            complement(2375..5023)
                     /locus_tag="PLI30_07655"
     CDS             complement(2375..5023)
                     /locus_tag="PLI30_07655"
                     /inference="COORDINATES: protein motif:HMM:NF014026.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 C-terminal
                     domain-containing protein"
                     /protein_id="HOI79463.1"
                     /translation="MKKTSVSYLFLLWIAGFLFFSCKEVQPYLNTKLSFEERADDLLS
                     RLTIEEKAELMRYDSPAIDRLGIPAYNWWNECLHGVGRSGLATVFPQAIGMAAMWDSQ
                     QMSEIATAISDEARAKHHDYVSKGKRGIYQGLTFWTPNINIFRDPRWGRGMETYGEDP
                     FLTAELAIPFIKGLQGDDSKYLKLVATVKHFAVHSGPESTRHSFEVYPSDYDLSETYL
                     PHFRRAVQEAGAYSVMCAYNRLRGEPCCGDKYLEDLLRNKWGFNGYIVSDCGAISDFY
                     RENAHHIVNTPEEASAKAVKAGTDLNCGDTYAKGLIEAVKQNLITEAELDVSVKRLLL
                     ARFKLGMFDPDSEVKYAQIPIEVVDSENHRVLALQAARKSMVLLKNDNNLLPFSKEVK
                     KVAVIGPNADDNEVLLGNYNGYPSKGITPLKGIIEKLPHAEVAFAQGCTLAEKLPYFT
                     VIPADYFYTDKTLQTKGLNVEYFDNNKLEGIAKHTRVDDNIDFVWWNKAPFDDLNPEE
                     FSICWRGVLVPPVTGEYAIGGEAFTGYKLSVDGKVVTQGRDQHHARKRYEYVQFEAGK
                     PYEIEVEYVQDKTEYAHVKILWDVPNPNLKQEAIDLARNSDLVILCMGLSPLLEGEEM
                     KVNVEGFAGGDRLDIKLPAVQTELMKEIQKLGKPTVLVLLNGSALAFNWEAENISAIL
                     EAWYPGQSGGTAIADVIFGDYNPAGRLPVTFYKDVNQIPAFDNYDMQGKTYRYFEGKP
                     LYEFGYGLSYTTFQYKLRTVPESVKNGEDISVSVDVTNTGNMDGDEVVQLYVSLPDSK
                     LQKPIRTLQGFKRVHLKKGETQTVKFTLKPHQIAARNKANIPVVETGKVQVSIGGKQP
                     DAGSLATGNVVEVFIETEEDNYFINE"
     gene            complement(5056..5790)
                     /locus_tag="PLI30_07660"
     CDS             complement(5056..5790)
                     /locus_tag="PLI30_07660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007218454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HOI79464.1"
                     /translation="MKNSTHVYNPHVSVDCVVFGFDGEKLKVLLIERTIEEQNFSDKK
                     LPGSIIFEDEDLDEAAIRILNELTGLKNIYLSQFHSFGNPQRTKNMRDKNWLEKLTNM
                     KIGRIVTVGYVALIKISRKIIFESENTAANWYDVSSLKSLRLAFDHNEIADTALEHIR
                     HKVKSEPSMLFELLPQKFTMTQLRNLYDIIMGTTSDVRNFKKKIMQIEGLEQLDEVQK
                     DVPYRAPRLYRFDKKVHKKNTRKLYS"
     gene            6156..>6525
                     /locus_tag="PLI30_07665"
     CDS             6156..>6525
                     /locus_tag="PLI30_07665"
                     /inference="COORDINATES: protein motif:HMM:NF013665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase"
                     /protein_id="HOI79465.1"
                     /translation="MIHKLAELTEKAKQKPQRRIAVAAAEDESVLKSLKAAMQDGLVS
                     PILIGHKSEIEKLAELVDFDLSQVEIIHNDNGAHESAKIAVSVIKKGGADILMKGLVS
                     TGDLLKAVLDKENGLRKAAIL"
BASE COUNT         1670 a         1592 c         1322 g         1941 t
ORIGIN      
        1 tgcaggtcgt gttcaaaggt gtgtccggcc aatgtggcat ggttcacctc aatgttcagt
       61 ttgaaatcct tgtcgaggtt gtgtgcgcga agaaatccaa tcacggtttc tgcatccgta
      121 tcgtactggt gtttggtcgg ctccatcggc ttgggttcaa tgagaaatgt acctttgaat
      181 ccttttgagc gagcataatc gcgtgccttg gccagcatca tggccaggtg ttccttttct
      241 cgcttcatat cggtgttcag gaggctcata tacccttcgc gcccgcccca gaaaacataa
      301 ttttcccctc caagctcgat ggtggcatca agtgcgtttt tgatctgtgt ggcagcgtag
      361 gcaacactgt cgaagttggg gttggtggct gccccgttca tgtaacgctt gtgactaaaa
      421 acattggctg tgccccagag cagtttgatg cctgtctctt ccattctttt tttagcgtat
      481 gcaacgatgg acctgaggtt agcttcatac tcccttatgg tttttccttc gtcgatcaga
      541 tcgatatcgt gaaaacagaa atattcgatt ccaatcttct gcataaactc aaagccggca
      601 tctagcttct tccgagctct ttccaaagca ccattgccat tgttccattc aaaatctttg
      661 gtttcttctc caaaagggtc gctcgattcc gcacaaaggg tatgccagta agccatggag
      721 aatttgaacc aatctttcat ctttcgtccg taaaccactt tttcagcatc gtagtaacga
      781 aatgcaagcg ggtttctgct ttcttttcct tcaaaccgaa tcttgccgat tccgggaaaa
      841 aattcttttt ttgtttccat aattatttat tgtgtgttgt ttgctattga gttatgtatg
      901 ttttccaacg ttggtaagcc tcctcatacc tctctttgtc cctttcatcg gacccgatgg
      961 tcatgatctt cttcagcgaa gcaaatgcct cttcgttgga agaataaatt cctacaccca
     1021 tgccggcagc ttttgccgcg cccgccgctc cgtcggtatc gaacagttca atggttgccc
     1081 cggtaacacc agccagtgtt tcccggaata caggacttaa gaacatattg gcatttccgg
     1141 ccctgataac attgatttcc atccccatct cttccatgat ttccattccg tacctgaagg
     1201 agaagacaat gccctcctgc gaagcacgaa caatatcaga aagggtatgg atattgaagt
     1261 tgatgccgtg aaaagaacat cccggattct tgttttccag cacccgctct gctccgttgc
     1321 cgaaggggat caccgagact ccctttgagc cgacaggaga tttttgggcg agttcgttca
     1381 tattgttgta atccaatccc ggctgtatca tgttcctttt tatccacgaa ttcagaatac
     1441 cggttccgtt gatacacaat aagacaccca gcctcggctt cccggatgtg tggttcacgt
     1501 gtgcaaaggt gttaacacgc gaaagcgggt cgtaattcac ttcgcccagc accccgtaaa
     1561 ccactcccga cgttcctgcg gtagaggcta tttctcctgg gttgaaaacg ttgagcgaaa
     1621 cagcattgtt gggttggtcg cctgcccggt atcccagcgg tgtaccttct ttcaagccaa
     1681 gttctatggc ggctttcgca caaaccgttc cctgaaaacc aaacgtcggg acggtcggag
     1741 ggatgatatc gttactaaag ccaaaataac tcattacctc tgtcgagaca gtatttctct
     1801 gaaaatccca gaaaacacct tccgataatc cttcaatcgt agtgcaaaca ccacccgtca
     1861 gcttcatggc aatataatca ccggggagca tgatcttatc gatttgtcca taaaggcagg
     1921 gttcattttc ttttacccaa gccagttttg atgcggtgaa gttacccggc gagttcaaca
     1981 gtgcagccag ggcattcttt gcccctatcg cctcgaatgc tttttcaccg tagggtacag
     2041 cgcgactgtc gcaccaaatg atggacgggc gaagcacttt tccgttctta tcgatggcga
     2101 ccaatccgtg catctgccac gatatcccga tgcctttgat ttcggcaaca tctatacccg
     2161 actttttaat taccgattgg tttgccagct tcaggttttc ccaccacatg tcggggtctt
     2221 gttccgccca acccggttgt gcagcataga tcggcatctc ggtcttcggg aaaaaatcgg
     2281 atgctaccac ctgccccgtt tctgcattca ccaggcaagc ttttaccgat gaactgccta
     2341 tatcgtatcc caacaaatac atgatgttct ctttttattc gttaataaaa taattatcct
     2401 cctctgtctc aataaaaacc tctactacgt ttccggtagc cagcgaaccg gcatcgggtt
     2461 gcttacctcc gatcgacacc tgcacttttc cggtctcaac aaccggtatg ttcgctttgt
     2521 tccttgcagc aatctgatgc ggtttcaacg taaacttcac ggtttgtgtt tcgccttttt
     2581 tcaaatgtac ccgtttaaat ccctgcaaag tacgaatcgg tttttgcaat ttactgtcgg
     2641 gtagagacac gtaaagttga acaacttcat ctccatccat attgccggta ttggtcacat
     2701 cgacagaaac cgaaatatcc tctccgttct tcacgctttc gggaacagtg cgcagtttat
     2761 actgaaaggt ggtataactt aacccataac caaactcgta cagaggtttt ccttcaaaat
     2821 accggtaagt tttaccctgc atgtcataat tatcaaaagc aggaatctgg ttcacatctt
     2881 tataaaaggt aaccggaagg cggcctgccg gattgtaatc gccaaaaata acgtcggcaa
     2941 tggctgttcc gcccgactgc ccgggatacc atgcctccag aatggcagaa atattttcag
     3001 cttcccagtt aaaggctaag gcgcttccat tgagaaggac cagaacagtg ggtttcccga
     3061 gtttctgaat ttctttcatg agctcggttt gaacagcggg tagtttgatg tcgagacggt
     3121 ctccacccgc aaaaccttct acattgactt tcatctcttc gccttccaat aaggggctca
     3181 gtcccataca cagaatcacc agatcggagt tcctggccaa atcaatggct tcttgtttca
     3241 aattaggatt aggcacatcc caaagtatct tcacatgagc atattctgtt ttatcctgaa
     3301 cgtattccac ctctatttca tacggttttc cggcttcaaa ctgcacatac tcataccttt
     3361 ttctcgcgtg atgctggtcc ctgccttgtg ttaccacctt accgtccacc gacagtttgt
     3421 atccggtgaa agcttcgcct ccaatggcat attcacctgt aacaggtggc acaagaactc
     3481 ctctccagca aatcgagaat tcttcagggt ttaaatcatc gaagggtgct ttattccacc
     3541 acacgaaatc gatattgtca tcaacgcgcg tgtgctttgc gataccttcc agcttgttgt
     3601 tatcgaaata ttcaacatta agtcctttcg tttgaagcgt tttatcggta taaaaataat
     3661 cagccggaat aacggtaaag tagggaagct tttccgccag tgtgcatccc tgagcaaaag
     3721 caacttcagc atggggtaat ttttcgataa tcccttttaa cggcgtgatt cctttcgaag
     3781 ggtatccgtt gtagttgccc aacagaacct cgttatcgtc cgcatttgga ccaataacgg
     3841 ctactttttt aacttccttg ctaaaaggca agaggttgtt gtcgttcttc aataaaacca
     3901 tcgattttcg tgcagcttgt aatgccagca cccgatgatt ctcactgtcc acgacctcaa
     3961 tagggatttg cgcatatttc acttcgctat ccgggtcaaa cattccgagc ttgaatctgg
     4021 caagcaaaag ccgttttacc gaaacatcca gctccgcttc cgtgattaga ttttgcttca
     4081 cggcttcaat caaacctttg gcatatgtat cgccacaatt aaggtcggtt ccggctttta
     4141 ccgctttagc cgatgcttct tcgggtgtgt tgactatgtg gtgggcattt tccctgtaaa
     4201 aatcactgat ggccccacaa tcggaaacaa tatatccgtt gaacccccat ttgttccgca
     4261 acagatcttc caggtattta tcgccacagc acggctcacc ccgtaaccgg ttataggcac
     4321 acatgacgga ataggcaccc gcctcctgga cagcacgcct aaaatgaggc agataagttt
     4381 ctgacagatc ataatctgaa ggatatacct cgaacgaatg ccgggtggat tccggaccgc
     4441 tgtgaacagc aaaatgtttg acggttgcca ctaacttcag gtactttgaa tcatcgccct
     4501 gtaatccttt tatgaaaggg atggcaagtt ctgccgtcag gaacggatcc tctccatagg
     4561 tttccatccc cctgccccac cgcggatcac ggaaaatatt gatgttgggt gtccagaaag
     4621 tcaatccctg gtaaatacct cttttccctt ttgagacata atcgtgatgc ttggcgcgcg
     4681 cttcgtcaga aatggctgtt gcaatttctg acatttgctg actgtcccac atagccgcca
     4741 ttccgatagc ttgtggaaaa accgtagcca aaccggagcg cccgacaccg tgcaagcatt
     4801 cgttccacca attatatgca ggtatgccca gccgatcaat ggccggtgaa tcgtatcgca
     4861 tcagctcagc cttctcttcg attgtcaacc gtgagagcag gtcgtccgct ctttcttcga
     4921 aagagagttt tgtgtttaaa taaggctgca cctctttgca ggaaaaaaag agaaatcctg
     4981 cgatccacaa aagaaacagg taggaaacag aagtcttttt catcgcaaac tgtattttga
     5041 gtttaacatg aacggctaac tatatagttt tcgtgtattt tttttgtgta cttttttatc
     5101 gaacctgtaa agcctgggag ctctgtaagg tacatctttc tgcacttcgt ccaattgttc
     5161 caggccttca atctgcatta tttttttctt gaagttacgg acatcagacg ttgtgcccat
     5221 tatgatatca tacaggtttc taagttgtgt catggtgaac ttctgaggca gcagttcgaa
     5281 taacatggag ggctccgact tcactttgtg gcggatatgc tctaacgcag tatccgcaat
     5341 ttcattgtga tcgaaagcta gccgtaaaga ttttaatgaa cttacgtcgt accagtttgc
     5401 tgcagtattc tccgactcaa aaataatttt tctacttatt ttaatcagtg ccacataccc
     5461 tactgtaacg attctgccga ttttcatgtt tgtcagtttc tccagccaat ttttgtccct
     5521 catattcttt gtcctttggg gattgccaaa gctatgaaac tggctcaggt aaatgttttt
     5581 taatcccgtc agttcgttaa ggatacgtat agctgcctca tccaggtctt catcttcgaa
     5641 aataatgctt cccggtaatt ttttatcgct aaagttttgt tcttctatcg tccgttcgat
     5701 aagcaatact ttcagttttt caccgtcaaa cccaaaaaca acgcagtcca ccgaaacgtg
     5761 agggttgtaa acatgagtgc tatttttcat gacatattct taataagaat acaaaattat
     5821 acagattaat cacaatacat gtaaaatttt tatatgtttt aaaacattat tatcagcaat
     5881 ttatataaag taaaaaatac atatataaaa ataaccttat tgtaattatt acttatatta
     5941 catcttgaaa actacttcga attactttta tagctttacc tttttatgaa aaataatatt
     6001 catgaatagt tttatatata tgaatagctt aggagaaaag tttcctgcca aaagaaacag
     6061 atttctgaca taattccaat aaaaaacttg tttcaggaga ttatcttctt ttttttttgt
     6121 tattttgcgt gtaaatctcc ttaataatac tttttatgat tcacaaatta gctgagctta
     6181 cagaaaaagc caagcagaaa ccccaacgaa gaattgctgt cgcagcggca gaggacgaat
     6241 cggtactgaa gtctctcaaa gccgctatgc aggacggact tgttagcccc atactgattg
     6301 ggcacaaaag tgaaattgaa aaactcgcag agctcgtcga ttttgatctg agccaggtag
     6361 aaattatcca caacgataac ggagcccatg aatcggcgaa aatagccgtc tccgtgataa
     6421 agaaaggcgg tgccgatatc ctcatgaagg gacttgtcag cacaggcgac ctgttaaaag
     6481 ctgttcttga taaagaaaac ggcctgcgaa aagcagctat cctca
//
LOCUS       DAOSDR010000169         6521 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrimonas sp. isolate AMR_MDS_1826
            NODE_4276_length_6521_cov_4.067430, whole genome shotgun sequence.
ACCESSION   DAOSDR010000169 DAOSDR010000000
VERSION     DAOSDR010000169.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863355
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrimonas sp. (wastewater metagenome)
  ORGANISM  Petrimonas sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Dysgonomonadaceae; Petrimonas.
REFERENCE   1  (bases 1 to 6521)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6521)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:10:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,720
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 35
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6521
                     /organism="Petrimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4276_length_6521_cov_4.067430"
                     /isolate="AMR_MDS_1826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2023866"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..423)
                     /locus_tag="PLI30_07670"
     CDS             complement(<1..423)
                     /locus_tag="PLI30_07670"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004301093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthetase"
                     /protein_id="HOI79466.1"
                     /translation="MNVDVILGLQWGDEGKGKVVDVLTPNYDIVARFQGGPNAGHTLE
                     FENQKYVLRSIPSGIFQPGKINIIGNGVVLDPALFKAEVEALEASGHALTERLYVSKK
                     AHLILPTHRLLDEAMEQAKGDSKIGTTGKGIGPTYTDKI"
     gene            complement(494..937)
                     /locus_tag="PLI30_07675"
     CDS             complement(494..937)
                     /locus_tag="PLI30_07675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019540381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HOI79467.1"
                     /translation="MQYLSDRKHRKTPERFAILDHIYNTKGHFDMDSLYKSMVDVNFR
                     VSRATLYNTIELLLDCGLVVKHQFGGNISKYERAYGNENHDHIICTSCGEVWESKNGN
                     LFTAAQQRKIKKFTVSYYSMYIYGICSKCSHAKKIALRKLNKETK"
     gene            complement(1057..2508)
                     /locus_tag="PLI30_07680"
     CDS             complement(1057..2508)
                     /locus_tag="PLI30_07680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006841860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 10 glycosylhydrolase"
                     /protein_id="HOI79468.1"
                     /translation="MNRTHLLLIFFLFVVMRIFSVNPPASEVRAVWLTTNYGLDWPRN
                     RISQEVQKRELIAILDNLQKHNFNTVIFQVRARGEMFYDSKIEPLASVIVPSGYGKPK
                     FDPLAFVIEECHKRGMELHAWMVTYPLGLDKHVRSLGARSVTKKNPSITRKYRGEWFL
                     DPGNPKTDDYLLSIVKEIVSNYDIDGIHFDYIRYPDNTGKFPDAGMYRLYGNGKTLHQ
                     WRRDNITRFVTKAYDWVKSQKKWVQVSSAPLGRYRALGNTGAGWTALETVFQDAAMWL
                     KIGKHDALYPMMYYKDHLFYPFVDDWLENGNKRIIVPGLGTYQMIELGWSRQDIENQM
                     DYTRKNESAGQAYFRTDNVLSNTKGILNTLEHYYRYPAKLPAMTWLSDEIPGAPYDLT
                     AENINGKLLLSWKTDDAADRVTYNVYRSESEDLDVHKAENILATGLREPFFEYAASID
                     EKGYYYYVTVSDSYHNESEVCYPAFFVHSETVK"
     gene            2788..2997
                     /locus_tag="PLI30_07685"
     CDS             2788..2997
                     /locus_tag="PLI30_07685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005858259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HOI79469.1"
                     /translation="MNPLLFNLNGWEIPIIILVVLILFGGKKIPEFMKGLGKGINSFK
                     KGLNDIEEDIKADPADKKTSSTSNQ"
     gene            3096..3923
                     /gene="tatC"
                     /locus_tag="PLI30_07690"
     CDS             3096..3923
                     /gene="tatC"
                     /locus_tag="PLI30_07690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019538896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HOI79470.1"
                     /translation="MKEKEMSFWDHLEEFRWTLIRIIGAVLILAIAGFILIPRIFDSI
                     ILAPSSSDFITYRFLESASKYIPFLPDFSADSFNVEMLNINMTTQFMTYISTSLAFAV
                     LLTIPYILFELWRFISPALYENETKGVKMAFGFGGVMFVIGCLVGYFMVFPLIFRFLI
                     TFELSDAIKNTISLDSYMNNFYTLILIMGVVFELPLVFWLLSSLGLIYRSFFRKYRKH
                     AVVGSMVLAAIITPSGDPFSLIIVTIPLYMLWEISAFVVKKDPPEEIEEEDLPTVFE"
     gene            complement(3924..4298)
                     /locus_tag="PLI30_07695"
     CDS             complement(3924..4298)
                     /locus_tag="PLI30_07695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019538897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HOI79471.1"
                     /translation="MRTLGNVIWIVFGGIFIAIEYVIASIGLMVTIIGIPFGLQSLKL
                     AEMALLPFDKKAVQNRTTSGCLALVMNIIWFFIGGLPIVLTHLFFGVLFYITIIGIPF
                     GNQHFKLMKLAFSPFGKVILNR"
     gene            complement(4295..4672)
                     /locus_tag="PLI30_07700"
     CDS             complement(4295..4672)
                     /locus_tag="PLI30_07700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007570487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HOI79472.1"
                     /translation="MKKVISTKHAPAAIGPYSQAIEAKGMIFISGQTPIDPLTGELVT
                     GGVKEQAVQCFKNIRAILDEAGLTTENIVKTTVFLGDMAYFAEMNEVYASQFDGDFPA
                     RSAIAAKGLPKSCMVEIEAIAVR"
     gene            complement(4754..5497)
                     /gene="rlmB"
                     /locus_tag="PLI30_07705"
     CDS             complement(4754..5497)
                     /gene="rlmB"
                     /locus_tag="PLI30_07705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007481993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HOI79473.1"
                     /translation="MKEKEMIFGIRAVIEAAEAGKDIDKVLVKRELSGELFKELQEVL
                     RRCEIPMQKVPLERIDRITRKNHQGVIAFTSAVTYQKLDQIVPFLYEQGKNPLIVVLD
                     GITDVRNFGAIARTCEVAGVDAIVIPARGSVSVNADAIKTSAGALHIIPVCRENSLKD
                     AVVFLKNSGIRVVAATEKAAKNYTVADMSVPVAILMGSEDEGVSPEILRVCDELVKVP
                     QFGSIQSLNVSVAAGVMIYEVVRQRSIDY"
     gene            complement(5543..>6521)
                     /locus_tag="PLI30_07710"
     CDS             complement(5543..>6521)
                     /locus_tag="PLI30_07710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HOI79474.1"
                     /translation="FAVVDALSGEGGAVEQKLKSVTDALKSIQHVFPKAAELEQRVES
                     AYIDLKEVRIEAGKHFEETDFNAERQQQVEERLSVIYDLQKRHSVKSVEELQEIQKQL
                     DEKLQNILSLDEQIEQLEKEVSEKQRLLLQKAEVLSERRKSATQPVEKQLTEMLVYLG
                     MPNARFICDITTKNNPDSNGKDAVSFLFSANKNTPLLPVSQIASGGEISRLMLCLKSM
                     IAGATTLPTVIFDEIDTGTSGEIADKMGTIMREMAHDMQVIAITHLPQIAAKGTKHYN
                     VYKEDSAETTATYVRLLLKNERVTEIARMLSGAEVTVQAVENAKVMLEA"
BASE COUNT         1704 a         1571 c         1241 g         2005 t
ORIGIN      
        1 tattttatcg gtgtatgtcg gcccgattcc cttgccggtc gtccctattt ttgaatctcc
       61 tttagcctgt tccatagcct cgtctaacaa ccgatgggta ggcagaatta aatgagcttt
      121 cttggaaaca tacagccgct cggtcaacgc atgccccgat gcttccaacg cttctacttc
      181 agccttgaac agagccgggt ccagcacaac cccatttccg ataatgttga ttttcccggg
      241 ttgaaaaatt cccgagggaa tcgatctcag tacatatttc tgattttcaa actccaacgt
      301 atgaccggca tttggcccgc cctgaaaacg agccacaata tcgtaattgg gcgttaatac
      361 atctacaact tttccttttc cttcatcgcc ccattgaagg cctaaaatta catctacatt
      421 catattattc tttagattac aagactcaaa ggcttataaa catacatact attggtctat
      481 acgtctaaat gtcttatttt gtttctttat tcagttttct caaagctatt ttcttggcgt
      541 gactgcactt actgcaaatc ccgtaaatat acatactgta gtaacttacg gtaaattttt
      601 taatttttcg ttgctgtgcc gcagtaaaca ggttgccgtt tttggattcc cagacttcac
      661 cacaactggt gcagataatg tgatcgtgat tttcgttgcc gtaagcacgt tcgtacttcg
      721 aaatatttcc tccgaactga tgtttcacca ccagtccaca atcgagcagc aattcaatgg
      781 tattatagag tgtggcacgg ctgacacgaa aattcacatc taccatggat ttatataacg
      841 aatccatgtc gaaatgtcct tttgtgttgt aaatatgatc gagtatggca aatctctccg
      901 gagttttacg gtgcttacga tcagacagat actgcatgaa ctcatctttc acctgttttc
      961 tcaatttctg gctgctatcc atagtacaaa aatatttctt ttttcaatcg cttgaagtta
     1021 tttttttgat atttttttaa agatacacat ttaaattcat ttcaccgttt cggaatgaac
     1081 aaaaaatgcc ggataacaga cttcactctc gttatgatat gaatctgaaa ccgttacata
     1141 ataataataa cccttttcat caatggatgc tgcatactca aaaaaaggtt ctctcaatcc
     1201 cgtggcgagg atattttcgg ctttgtgtac atctaaatcc tccgattcac tcctgtaaac
     1261 attataagtg actcggtccg cggcatcatc ggttttccag ctaagcaaaa gttttccgtt
     1321 tatattttca gcagttaagt cgtacggtgc tccagggatt tcatccgaaa gccaggtcat
     1381 tgccggcagt tttgccggat aacggtaata gtgctccagg gtattcaaga tcccttttgt
     1441 gttcgacaac acattatctg ttctgaagta tgcctgtccg gccgactcgt tcttccttgt
     1501 gtaatccatc tggttctcga tatcctgccg gctccagccc agctcaatca tctgataggt
     1561 tcccaatccc ggaacgataa tgcgcttgtt cccgttttcc agccaatcgt ccacaaacgg
     1621 ataaaaaaga tggtccttat aatacatcat gggataaagg gcatcatgct taccaatctt
     1681 caaccacatg gcggcgtcct gaaaaacagt ttccaaagca gtccatccag cccctgtatt
     1741 ccccaacgcc ctgtaccgcc caagaggggc actgctgacc tgtacccatt ttttttgact
     1801 tttcacccag tcgtaagcct tggtcacaaa ccgggtaatg ttatctctac gccattggtg
     1861 taacgttttc ccgttaccgt aaagcctata catcccagca tcaggaaact tacccgtgtt
     1921 gtccggatac cggatataat caaaatgaat cccgtcaata tcataatttg aaacgatctc
     1981 ctttacgatg gacaagagat agtcgtcggt ttttggatta cccggatcaa gaaaccattc
     2041 gccacggtat ttccgggtta tggatggatt tttttttgta accgatcttg cacccaaact
     2101 tctcacatgc ttgtctaaac ccaacggata ggttaccatc caggcatgca gttccatacc
     2161 gcgcttatga cactcctcaa tgacaaaagc cagcggatca aatttgggct tcccatatcc
     2221 ggatggaaca attaccgatg ccagcggctc aatttttgaa tcatagaaca tttctcctct
     2281 tgcccgaacc tgaaatatta cggtattgaa attgtgtttt tgcagattat caagaatagc
     2341 gatcagctct cttttttgca cttcctgact gatcctgttc cttggccagt caagtccgta
     2401 atttgttgtc aaccagacgg ccctgacttc agaagcaggg ggattgacag aaaaaattct
     2461 catcacaaca aacagaaaaa agatgagaag cagatgtgtt ctattcatta attataatcg
     2521 ttctaaactt ggcgatgcaa atttaaaaca aaaatgcagt gctggtatac atttttcgaa
     2581 tgttaacact ttaaaccgga aaagccgatc aattctgtgt agaaacagga cttcaacacc
     2641 tcgctaaaaa gtagccacct cataaaatca tttcacggct ctttttacga aactttatct
     2701 taatattatt gctaaactta ataattaatt ctacttttgt gaaaatatag tgtttgcaaa
     2761 caaattatca aactaacaat aagacctatg aatccactgc tttttaattt aaacggatgg
     2821 gaaattccca ttattatctt ggttgtctta attcttttcg gtggaaagaa aatccctgaa
     2881 tttatgaaag ggttgggaaa aggcatcaac agcttcaaaa aaggactgaa cgatattgaa
     2941 gaggatatca aagccgatcc ggccgacaaa aaaacatcgt ccacaagcaa tcagtaacct
     3001 aattttccag ttattgtaaa aatcgtctgt tctcgggatt aatccacgtt tttgtggatt
     3061 ttttccgaga ttaaattcta taatctgttg gttgcatgaa agagaaagaa atgtcctttt
     3121 gggaccatct cgaggaattc cgctggacgt tgatacgaat tatcggcgca gtgcttattt
     3181 tggccattgc tggattcatc cttattcctc gtattttcga ctcaataatt cttgcaccca
     3241 gttcttccga ttttatcacc tatcgtttcc tggagagcgc cagcaaatac atccctttcc
     3301 ttcccgattt ttcggccgac tcgtttaatg ttgaaatgtt gaacatcaac atgaccacgc
     3361 agtttatgac ctatatttcc acttctctgg catttgctgt cctgttgacc atcccctata
     3421 ttttgtttga actctggcgc tttatcagcc cggctcttta cgaaaacgag accaaagggg
     3481 taaaaatggc ctttggattc ggaggagtga tgttcgtgat cggctgtctg gttggctatt
     3541 ttatggtttt tccattgatc ttcaggttcc tcatcacttt tgaattgagc gatgccatta
     3601 aaaacaccat ctcgctggat tcctacatga acaattttta caccctcatc ctcattatgg
     3661 gtgtcgtgtt tgagttgccg cttgttttct ggcttttatc cagcttggga ctcatctacc
     3721 ggtctttctt ccgcaagtac cgcaaacatg cagtggtagg ttctatggtg ctggcagcca
     3781 taattacccc gtctggcgac ccgttttcac ttattatagt caccataccg ctttacatgc
     3841 tttgggaaat aagcgctttc gtggtaaaaa aagatccacc ggaagaaatt gaagaagagg
     3901 atctgccgac agtctttgag taattatcgg ttcagaatca ctttgccaaa cggtgaaaaa
     3961 gctaatttca ttaatttgaa atgctggttt ccgaaaggaa taccgataat ggtgatgtaa
     4021 aacaaaacac cgaaaaacag gtgtgtgagc acaatgggca acccaccgat aaagaaccag
     4081 ataatattca taacaagtgc caggcacccc gaagttgtgc gattttgtac tgctttttta
     4141 tcgaaaggca acaatgccat ctccgccagt ttcagcgatt gcaggccgaa gggaataccg
     4201 attattgtaa ccatcaggcc aatactggca ataacgtatt cgatggcaat aaatatgcct
     4261 ccgaaaacaa tccagatcac atttcccagc gtcctcatcg tacggcaatt gcttcaattt
     4321 ccaccatgca gcttttaggc aagcctttgg cagctatggc cgaacgggca ggaaaatccc
     4381 cgtcgaactg cgaagcgtac acctcgttca tctcggcaaa gtaagccatg tctcccagga
     4441 agacggtagt ttttacgatg ttttccgttg taagtccggc ttcatccaaa atagcccgga
     4501 tatttttgaa acattgcacc gcttgctctt tcactcctcc agtgaccagc tctcctgtta
     4561 acggatcaat aggggtttgt ccggaaataa aaatcattcc ctttgcttcg attgcttggc
     4621 tgtaagggcc tattgccgca ggagcatgtt ttgttgaaat tacttttttc ataaaattta
     4681 gtttacttaa tattgctttc cttccacttt aattcatccg gttataatgt tgaaatatgg
     4741 gttgtattat tagttaatag tctattgatc tttgtcttac cacctcataa atcatcacgc
     4801 ccgccgccac agaaacgttc agtgattgaa tgctgccgaa ctgcggaacc ttaaccaatt
     4861 catcacacac acgcagaatt tcgggcgaaa cgccttcgtc ttccgaaccc attaaaatgg
     4921 ctacagggac cgacatatct gctacggtat agttttttgc cgctttctca gtggcggcca
     4981 ctactcttat gccactgttt ttcaggaaca caacagcgtc tttcaggctg ttttcccggc
     5041 ataccggaat gatgtgtaat gctccggccg acgttttaat ggcatcggca tttaccgata
     5101 cgcttccgcg cgccggaatc actatggcat ctacacccgc aacttcgcat gtgcgggcaa
     5161 tagcgccaaa gttacgcaca tcggtaattc catcaagtac aacaataagc gggtttttcc
     5221 cttgctcata aagaaacgga acaatctggt ctagtttctg gtacgtaact gccgaggtaa
     5281 aggcaataac cccctgatga tttttacggg ttatcctgtc gatccgctcc agcgggactt
     5341 tctgcatggg gatttcgcac cggcgtagca cctcctgcag ttctttgaaa agttcacccg
     5401 acaattcacg tttaacaagc actttgtcga tatcttttcc ggcttctgcc gcttcaatca
     5461 cggcacggat gccaaaaatc atctctttct ctttcatttt ttatgagtta tgtgttacgt
     5521 ctttaaagtt tcaacatgag cttcaggcct ccagcatcac cttggcattt tcaacagcct
     5581 gaacagtaac ttccgcaccg ctaagcatcc gggcgatttc cgtaaccctt tcgtttttca
     5641 gcaataaacg cacataggtt gccgtagtct cagcactgtc ctctttgtaa acgttgtaat
     5701 gttttgttcc tttggctgct atttgcggca aatgggtgat ggcgattact tgcatatcgt
     5761 gtgccatctc gcgcatgatc gtacccattt tatcggctat ctcacccgaa gttcccgtgt
     5821 cgatttcatc gaaaataacc gtcggcagtg tcgttgcccc ggcgatcatc gatttcaggc
     5881 aaagcatcag tcgagagatt tctccgccgg atgcaatctg ggaaacgggc agcaacggtg
     5941 tgtttttatt ggccgaaaac agaaagctca cagcatcttt gccgttagaa tcgggattgt
     6001 ttttggtggt aatatcgcat atgaacctcg cgttaggcat tcccagataa acaagcattt
     6061 ccgtgagttg cttttctacc ggctgcgtag ccgattttct cctttcactc agcacttctg
     6121 ctttttgcaa caacagccgt tgtttttcag acacctcttt ttcgagttgt tctatttgtt
     6181 cgtccagcga caggatgttt tgcaactttt catccaactg tttctgtatc tcctgcagtt
     6241 cctcaaccga ttttaccgaa tgcctcttct gtaaatcgta aatcacactt aaccgctcct
     6301 ccacctgttg ttgccgctcg gcattgaaat ctgtttcttc gaaatgcttc ccggcttcaa
     6361 tccggacctc ttttaaatca atataggccg actccacccg ttgctccaat tcggcagctt
     6421 tcggaaaaac atgctgaata cttttcaacg cgtcggttac cgattttaat ttttgttcaa
     6481 cagcaccacc ttcacccgaa agtgcatcta ctaccgcaaa a
//
LOCUS       DAOSDR010000170         6519 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrimonas sp. isolate AMR_MDS_1826
            NODE_4281_length_6519_cov_3.241027, whole genome shotgun sequence.
ACCESSION   DAOSDR010000170 DAOSDR010000000
VERSION     DAOSDR010000170.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863355
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrimonas sp. (wastewater metagenome)
  ORGANISM  Petrimonas sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Dysgonomonadaceae; Petrimonas.
REFERENCE   1  (bases 1 to 6519)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6519)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:10:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,720
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 35
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6519
                     /organism="Petrimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4281_length_6519_cov_3.241027"
                     /isolate="AMR_MDS_1826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2023866"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..206
                     /locus_tag="PLI30_07715"
     CDS             <1..206
                     /locus_tag="PLI30_07715"
                     /inference="COORDINATES: protein motif:HMM:NF018652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="coproporphyrinogen III oxidase"
                     /protein_id="HOI79475.1"
                     /translation="LTGLRTMWGVDLNLLKEKFGQELFDYCLQNARKFIREGFLVSKD
                     NSLIMNRKGIFISDGIMSELMRV"
     gene            complement(166..984)
                     /locus_tag="PLI30_07720"
     CDS             complement(166..984)
                     /locus_tag="PLI30_07720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008771887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HOI79476.1"
                     /translation="MKSKKMIVIYGVLLVVALSLMVILYTVSRKVEIVLPRDFSEIVN
                     SGELNIVTDYNPIGYHVSGDTLAGFQHGLIKALEQDWKLKVNIFLENSLDKNLQGLKQ
                     GRYDIVARNIPVTTHLRQQFGFTESINLNKQVLVQRKPEFNDSITPIRQHLDLAKKTI
                     HIHRDSPVILRLQNLSHEIGDTIFIIQNDIYETEQLVMMVAGGDIDFTVCDEVLARKL
                     AKEMPEIDVDTDISFTQLESWAVRKDSPVLLDSLNAWLSRFLTPASTHSLSHRK"
     gene            complement(996..3047)
                     /locus_tag="PLI30_07725"
     CDS             complement(996..3047)
                     /locus_tag="PLI30_07725"
                     /inference="COORDINATES: protein motif:HMM:NF025486.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative porin"
                     /protein_id="HOI79477.1"
                     /translation="MKGKLLILLFFCVSTTLVFSQARVILPDSSQITTHPDSVNLLPR
                     QAPSGLSRGPEEPFDTVNLELPAATVWHLHPRSGDRIVVPMDTLKHNFQQTTVPDGYS
                     VAGGYLGPIGSPFISKLFFERDETSQFPFYDAYKPYNKSPRQQVFYNTRVPYARLDYQ
                     TAGSRPLREQRLRALLTTNFGKRLNVGLEGDLISAKGLYKSQETKHNDWSFFGNYLSD
                     RFETHLLVSTSTAKQFENGGVTDERFIRDPESVGQNFSTVDIPVKFTDTWNKMKTNHL
                     FFSGKYNIGYNDQKKDSLGNGEFIPVASVIFTSHYTGQDRRFLSYDTVQVTVEGKEMQ
                     RIDQFYPNRFYNTAVDDSTTFSSLKNTFALSLREGFKPWVKFGLTGYIEHELRNFSMM
                     DSINGTIDRRKHRENSLFIGGILNKQQGENLRFNLQANLGVVGVDLGEFNLEGEVETG
                     FTIAGKRTVLSGHAYIKNLTPNYLQKHYHSKYFWWNKDFNNMRRVFVGGKLYIPFTNT
                     TLSVGVENLQNYIYFDKDRNSAQYSSNVQVLTVGVEQNLRLGVFNWDNRVVYQTSGNQ
                     EVIPVPTVSIYSNMYLKALIVNELTFQFGVDAHYHTAYFSPGYEPALLQFYNQREKEI
                     GNYPIATVYANMHLKQTRFFVMLYNAASAVWKPRDYFSLPGYPVNPMMLKLGLSVDLH
                     N"
     gene            3374..4231
                     /gene="hisG"
                     /locus_tag="PLI30_07730"
     CDS             3374..4231
                     /gene="hisG"
                     /locus_tag="PLI30_07730"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019539143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HOI79478.1"
                     /translation="MEKLKIAIQKSGRLSEKSLQLLNECGIKVSNGDRKLRTEARNFP
                     MEILFLRDDDIPQYVENGVADIGILGENEVWEKEKDVDEIEKLGFGNCRLSLAIPKDE
                     VYTNLDYFHGKRIATSYPKILKKYFGVKGIDVKIEELGGSVEIATGIGLADGIFDIVS
                     TGSTLIMNGLKEVEAIIKSEAVLIANKSLNEVKQELLERFLFRIRAYKKGMGSKYIVM
                     NAPNSSIPKICSILPGMKSPSILPLAEEGWSSVHSVVQEDQFWDVIDALKDVNAQGIL
                     VMNIEKMIL"
     gene            4234..5547
                     /gene="hisD"
                     /locus_tag="PLI30_07735"
     CDS             4234..5547
                     /gene="hisD"
                     /locus_tag="PLI30_07735"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019539142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HOI79479.1"
                     /translation="MKTLNNPAERKWPQLAERSAIKQARLMELVDKVFYDIRKKGDKA
                     VLKYARQFDRFSADDFTVDHETIEAASQQVSERLKQAIALAKSNIEKFHFSQKEEIVK
                     VETSPGVVCWREARPIEKVGIYVPGGTAPLFSTVLMLAIPAKIAGCKEIVLCSPPGKE
                     GAIHPAILYAANLAGVTRIFAVGGIQAIGAMSFGTKSVPKVEKIFGPGNQYVMTAKHA
                     AQYYGTAIDMPAGPSELLVVADETCRPSFVAADLLSQAEHGPDSEVILLSDNKKVVKE
                     INKELDIQLANLPRKEMAEKALKNSRAILFKDVETCIRFSNVYAPEHLILAVENPVLR
                     SREVTNAGSVFLGNYSCESAGDYASGTNHTLPTSGYAKAYSGVSLDSFIKKITFQQLS
                     EEGIVGIGVAVETMAEAEELFAHKNAMTLRLGEVSAQQAKNYENS"
     gene            5534..>6519
                     /gene="hisC"
                     /locus_tag="PLI30_07740"
     CDS             5534..>6519
                     /gene="hisC"
                     /locus_tag="PLI30_07740"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004290345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HOI79480.1"
                     /translation="MRTVDIEKMARPNILALKPYSSARDEFSGNEGIFLDANENPYGT
                     LNRYPDPHQKALKTKLSEVKNVPFNQIFIGNGSDEVIDLIYRIFCEPGKDSAIVCPPT
                     YGMYEVSANINHIQLHEIPLTSDFQLDIEKILQTAETDSSVKLIFVCSPNNPTGNRLD
                     NLEKLLENFNGIVVADEAYVDFCPEYSLKSKINKYSHLIVMQTFSKAWALAGVRVGMA
                     FAGEKIISLLDKTKPPYNVSIINQQVALEALSRVDEFEKRRDTILQQRAYLAEELSKL
                     EIVKKIYPSDANFLLVEVSDANRIYNELVQKKVIVRNRNRQVTNCVRITIGTP"
BASE COUNT         1831 a         1486 c         1575 g         1627 t
ORIGIN      
        1 ttctgacggg gttgcgcacg atgtggggcg tggacctaaa cctgctgaag gaaaaattcg
       61 gacaggaact gttcgattac tgcttgcaaa atgcgcgaaa attcatccgt gaaggctttc
      121 ttgtgtctaa agacaattcg ctgataatga atcggaaagg tatatttatt tccgatggga
      181 taatgagtga gttgatgcgg gtgtaagaaa tcgcgagagc caggcattca ggctatccag
      241 cagaacaggc gagtctttcc gcaccgccca cgactccaat tgcgtgaagc tgatgtcggt
      301 atcaacatct atctccggca tctctttggc aagttttcgc gcaagtacct cgtcacaaac
      361 cgtgaagtca atatccccgc ctgcaaccat catcaccagt tgttctgttt cgtatatatc
      421 gttttgtatt ataaaaatgg tatctcctat ttcgtgagaa aggttttgca gacgcaaaat
      481 tacgggcgaa tccctgtgta tatggatggt ttttttggct aaatccaggt gttggcggat
      541 gggtgttatg ctgtcgttaa actccggttt tcgctgtaca agtacctgtt tattcaggtt
      601 aattgactcg gtaaagccaa actgctgacg caaatgcgtg gttacgggaa tgttccgggc
      661 aacgatatcg taccgtccct gtttcagccc ttgaagattt ttatccaggc tgttctccag
      721 aaagatattt actttcagct tccaatcctg ttccagggcc ttgatgagcc catgctgaaa
      781 tccggccagg gtatcgccgg agacatggta tccaatggga ttataatcgg taacgatgtt
      841 caactcgccg ctgttcacta tctcggaaaa atctctgggt aaaacaatct ccaccttcct
      901 ggaaacggta taaaggataa ccatcaggga aagcgcgaca actaaaagta cgccgtaaat
      961 gacaatcatt tttttcgatt tcacagcaga gaaaattaat tgtgcaggtc aaccgaaagc
     1021 cccagtttaa gcatcattgg atttaccgga tagccaggca aagagaagta atcgcgcggt
     1081 ttccaaacgg cagacgccgc gttgtaaagc atcacaaaaa agcgggtttg tttcaggtgc
     1141 atgttggcat acaccgttgc aatggggtaa tttccgatct ctttttcccg ctgattgtaa
     1201 aattggagca atgccggctc gtatccgggg gaaaaataag cggtatgata atgtgcatca
     1261 actccaaact gaaaagttaa ttcattcact atcaatgctt tcagatacat gtttgaatag
     1321 atacttaccg tagggacggg gattacctcc tgatttcctg aagtttgata cactacccgg
     1381 ttatcccagt taaaaactcc cagtctcagg ttctgctcca caccaacggt caaaacctgt
     1441 acgtttgaag aatactgtgc actgtttctg tccttgtcaa aatagatata attctgcaaa
     1501 ttttctactc cgacactcaa tgtcgtattg gtaaagggta tataaagttt accgcctaca
     1561 aacaccctgc gcatattatt gaagtctttg ttccaccaaa aatacttgga gtgatagtgt
     1621 ttttgcaaat aattaggggt taggttctta atatatgcat ggccgcttaa gacagttcgt
     1681 tttccagcaa tggtaaaacc tgtttcaacc tcaccttcca ggttaaattc tccgagatcg
     1741 acccccacca ccccaagatt ggcctgaagg ttgaatcgga ggttttcgcc ctgttgttta
     1801 ttaagaattc caccgataaa aagcgagttc tcccggtgtt ttctccggtc aatagtcccg
     1861 ttgatggagt ccatcatcga aaaattacgg agctcgtgtt cgatatatcc cgttaatccg
     1921 aacttcaccc acggtttgaa tccttcgcga agcgataggg caaacgtgtt ttttaaggat
     1981 gagaacgttg tgctatcgtc gaccgcggtg ttgtaaaacc ggttggggta aaactgatca
     2041 atacgctgca tctccttgcc ttctaccgtt acctgcaccg tgtcgtaaga aaggaagcgg
     2101 cggtcctgtc cggtataatg cgacgtaaaa atgacactcg ccacgggaat aaactctccg
     2161 ttaccgagtg aatccttttt ctgatcattg tagccgatat tgtattttcc tgaaaaaaac
     2221 aaatgattgg tcttcatctt gttccatgta tcggtaaatt ttacaggaat gtctacagtc
     2281 gaaaagttct gtccaacgga ttccggatca cggataaacc gttcgtcggt aaccccgccg
     2341 ttttcaaatt gcttggcagt tgatgtcgat accaaaagat gggtctcaaa ccggtcgctc
     2401 aggtagttcc caaagaagct ccagtcatta tgcttggtct cctgcgactt gtataatcct
     2461 tttgcactga tcaggtcccc ctcgaggccc acattgaggc gttttccgaa gtttgttgtc
     2521 agtaaggccc taagacgctg ctccctcaac ggacggctgc ccgccgtctg gtagtccagt
     2581 cgggcatacg gcacccgggt attataaaag acctgttgcc tgggagattt gttgtagggt
     2641 ttgtaggcat cgtaaaacgg aaactggctc gtttcatccc tttcaaaaaa cagtttggat
     2701 ataaacggtg aaccaatcgg gccgagatac cctccggcaa ccgaataccc atctggaacg
     2761 gtcgtttgct ggaagttgtg cttcaacgta tccatgggca caacgatgcg gtctccgctt
     2821 cttggatgta aatgccatac cgttgctgcc ggcaattcca ggttgacggt atcgaaaggt
     2881 tcttccgggc cacggcttag gcctgacgga gcttgtcggg gtaacaggtt taccgaatcg
     2941 ggatgggtgg tgatctgcga cgaatcgggc aaaataacgc gcgcctgcga aaacacaagc
     3001 gtcgtactca cgcagaaaaa gagaagtatg agcagttttc ctttcatcgg tgcaaactta
     3061 cataaaaatt tgccacatgc cgatagctga cagcctattt gttgtgtttt ttcaggattt
     3121 ttaggaagca aaagaaaaat aaacagttat ttttgtcccg cattaaaatt attctattat
     3181 ctttgcgtta tcaaaataga taaaatgaaa gtaaacaagc ttcaccatca tcattttcat
     3241 acttgcaatc aggcgagctg aataggatgt gtagaagcat gaatatacaa catagacccg
     3301 tctgaacagc tcaggcgggt ttttttaatt aacactcagc ggttcggagc cgctaacgca
     3361 aaaaagaaaa gcaatggaaa aactgaaaat agccatccag aaaagcggac gattaagcga
     3421 aaaatcgttg cagctgttga acgaatgcgg cattaaagta tcgaacggcg accggaaact
     3481 caggacagaa gcccgcaatt tccccatgga aatccttttc ctacgtgacg atgatattcc
     3541 tcaatacgtt gaaaatggcg ttgcagacat cggtatcctg ggagaaaacg aagtgtggga
     3601 aaaagaaaaa gacgtggatg aaattgaaaa gctgggattc ggcaactgtc ggctttcgct
     3661 cgctattccc aaggatgaag tgtataccaa cctggattat tttcacggga aacgcattgc
     3721 caccagctat ccgaaaattc tcaaaaagta cttcggcgtt aaaggcattg atgtgaagat
     3781 cgaagaactg ggggggagtg ttgagattgc caccggaatt ggcctggccg atgggatttt
     3841 tgatattgta agtaccggca gcacccttat tatgaacggc ctaaaagaag ttgaggccat
     3901 cataaagagt gaagcggtac tgattgccaa caagagcctc aacgaagtca aacaagagct
     3961 gctggaaagg tttctttttc gcatacgggc atataagaaa ggcatgggta gcaagtacat
     4021 tgtaatgaat gcaccaaatt cgtcaattcc aaaaatttgt tccatccttc cgggtatgaa
     4081 atcgcccagt atccttccgc ttgccgaaga gggatggagc agcgtgcact cggtagtaca
     4141 ggaagaccag ttttgggatg tgattgatgc gttgaaagac gtgaatgccc agggaatctt
     4201 agtgatgaac attgagaaaa tgatactcta aaagtgaaaa cattaaacaa cccggccgaa
     4261 aggaaatggc cgcaattggc agaaaggtcc gccatcaagc aagccaggct gatggagtta
     4321 gtggacaagg ttttttacga tatccgtaaa aaaggtgata aggcggtgtt gaaatatgcc
     4381 cggcagtttg acaggttttc ggcggatgac ttcacggttg atcacgagac aatcgaggcg
     4441 gcgtcgcaac aggtctcgga acggttgaag caagccatcg cccttgcaaa aagcaacatc
     4501 gagaaattcc acttttcgca gaaagaggaa attgtaaaag tggaaacctc tcccggtgtg
     4561 gtatgctggc gcgaagcacg gcctatcgag aaggtaggga tttatgtccc cggaggaacc
     4621 gctcctcttt tttccacggt actgatgctg gccattccgg caaagatagc cggctgtaaa
     4681 gagatcgtgc tttgttcgcc tcccggcaag gaaggcgcta tccaccccgc cattctttac
     4741 gcagcgaacc ttgcgggggt aacccgcata tttgcggtcg gaggtatcca ggctatcggg
     4801 gccatgtctt tcggcacgaa gtccgtcccg aaagtagaga aaatattcgg cccaggcaac
     4861 cagtacgtga tgacggcaaa gcatgccgcc cagtactacg gtacagctat cgatatgcct
     4921 gccggaccca gcgaactcct tgtggtggcg gatgagacct gccgcccgtc gttcgtcgcc
     4981 gccgacctgc tttctcaggc agaacacggg ccggacagtg aggtcatcct tttgtcggac
     5041 aacaaaaagg tggttaagga aataaacaag gaactcgata ttcaactggc gaatctgccg
     5101 cgaaaagaaa tggcggaaaa agcgctgaag aacagccgtg ccattttgtt taaagacgta
     5161 gagacctgca tccgcttcag caacgtttac gctcccgagc acctgatcct ggcggtagag
     5221 aatcctgttt tgcgcagccg tgaggtgacc aacgcgggct ccgtttttct ggggaactac
     5281 agttgcgaaa gtgccggtga ttacgctagc ggcactaacc acacgcttcc cacaagcgga
     5341 tacgcgaaag catacagcgg cgtttctctg gacagcttta taaagaaaat cacctttcag
     5401 caactgtcgg aagaaggaat tgtcggtatc ggggtagccg tggaaaccat ggctgaagca
     5461 gaagaacttt tcgctcacaa gaatgccatg acactgcgtt tgggcgaagt gtcggcacag
     5521 caagcaaaga attatgagaa cagttgatat agaaaaaatg gccaggccga atatcctggc
     5581 actaaaaccc tattcaagtg cccgcgatga attttcgggc aacgaaggta tctttctcga
     5641 tgcaaacgaa aatccgtacg ggacactcaa ccggtatccc gacccccacc agaaagcgtt
     5701 aaagacgaag ctgtcagaag ttaaaaacgt acctttcaac cagattttta tcggcaacgg
     5761 aagtgacgag gtcatcgacc tgatataccg aattttctgt gaacctggaa aagacagcgc
     5821 gattgtttgt ccgcccactt acgggatgta cgaagtctcg gctaacatca atcacatcca
     5881 actgcatgaa atcccattaa catccgattt tcagcttgat attgaaaaga tcctgcagac
     5941 ggccgaaaca gacagttccg taaagcttat tttcgtttgc tcaccgaata accccaccgg
     6001 gaacaggttg gacaacctgg agaagctgct tgaaaacttt aacggaatcg tggtggcaga
     6061 cgaagcttac gtggattttt gccccgaata cagcctgaaa agcaaaataa acaaatactc
     6121 ccaccttatt gtgatgcaga cattcagcaa agcatgggcg ttggcgggtg tccgggttgg
     6181 aatggctttt gccggcgaaa aaatcatatc gttgctggac aaaaccaaac cgccctacaa
     6241 cgtcagtatc atcaatcagc aagttgccct ggaagcgctt tcgcgcgtgg atgaatttga
     6301 aaagcgccgc gacacgattc tgcagcaaag agcatatctc gccgaagaat tatcgaaact
     6361 ggaaattgta aagaagattt atccttcgga tgccaacttc ctcttggttg aagtatcaga
     6421 tgccaaccgg atttacaacg aactggtaca aaaaaaggtg attgtccgca accggaacag
     6481 gcaggtgaca aactgtgtga gaattaccat cggcactcc
//
LOCUS       DAOSDR010000171         6505 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrimonas sp. isolate AMR_MDS_1826
            NODE_4289_length_6505_cov_4.521860, whole genome shotgun sequence.
ACCESSION   DAOSDR010000171 DAOSDR010000000
VERSION     DAOSDR010000171.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863355
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrimonas sp. (wastewater metagenome)
  ORGANISM  Petrimonas sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Dysgonomonadaceae; Petrimonas.
REFERENCE   1  (bases 1 to 6505)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6505)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:10:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,720
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 35
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6505
                     /organism="Petrimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4289_length_6505_cov_4.521860"
                     /isolate="AMR_MDS_1826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2023866"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..417
                     /locus_tag="PLI30_07745"
     CDS             <1..417
                     /locus_tag="PLI30_07745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018359945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HOI79481.1"
                     /translation="AVRENFKRLIGIKNAAGVEVCQMERGLEVLIGVKKEPGFGHIIT
                     CGLGGIFVEILKDIQYTLAPVTRTEALRMIRSLKSYKLIQGARGKEGISEEVFAEVIC
                     KVSDLLVLVPEIEEMDLNPLMGRGHHLSAVDVVIKM"
     gene            424..1605
                     /locus_tag="PLI30_07750"
     CDS             424..1605
                     /locus_tag="PLI30_07750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI79482.1"
                     /translation="MSFITLLFIAVFPLHAQEPSDSAGSVSDDGLHRIHFVLNGQITG
                     WETTQLSNSVNWQPGCRFLPVLLGNFSWGENSKLDAEASVHLYGLLNFTGWDYINKEG
                     KLKPYRVWLRYSGKNWELRGGLQKINFGTARMFRPLMWFDAMDVRDPLQLTDGVYGIL
                     GKYFFENNASVWLWSLLGNKRPKGWELIGSARRRPEIGGRFEFPAFAGEMGISTHHRK
                     TDVHHLLPDVPENTLLDENRLGLDGRWDVGVGLWFETSFTRLQKNNYKMALHQDAINV
                     GMDYTFPLGNGLGLTVEYFRYHTGDGFMRKGMNLNVIGEMLSYPVSVLDNLTAMFFYV
                     GNDLNMWLNYISWARTYDKWSFYLMGFWNPEINLPLGGQAGGKNLFMGKGIQLMASYN
                     F"
     gene            1674..2393
                     /locus_tag="PLI30_07755"
     CDS             1674..2393
                     /locus_tag="PLI30_07755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019538572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOI79483.1"
                     /translation="MDSKNNLIVDVNGLVKRFPIGFSGFFTALKGIDLTVSEGEFMGL
                     VGPSGSGKTTFLNIIGTLDKPSEGRVNVLDKNAGTLTPRQAAQLRKEEMGFIFQSFNL
                     LPVYTVFENVEFPLLLLNLSYGERKKRVMETLEWLGLTSKINSRPPQLSGGECQRVAI
                     ARAMVKRPKLVLADEPTANLDADNSHRILKTMVKLNEELKTCFIFATHDEKVISYLKR
                     IVHLEDGRIVKDISRQKSELQ"
     gene            2406..3581
                     /locus_tag="PLI30_07760"
     CDS             2406..3581
                     /locus_tag="PLI30_07760"
                     /inference="COORDINATES: protein motif:HMM:NF014717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HOI79484.1"
                     /translation="MKILKLVFRNLIGNGLKTWLNVFVLSIAFVLIIFMQAILQGWDR
                     QAMSDTVKWEIADGQYWNANYDPFDPFTLDSAVSIIPKQLVNQYETGQVEPVLIVAGT
                     IYPSGRSMPVLMKGIRSGQQLLELPTSLLMNPTDSADIPAIIGSNMAHQSGLTENDIV
                     TLRWRDANGTFEARDIRIAGVFRTFVPTVDAGQIWLPLDILQKITLNSGKVSLLVKSK
                     EVSVVPVDGWNFKDVDELTRPLRETLRSKTVGQSVFYVIFLLLALLAVFDTQTLSIFY
                     RQREIGTFVALGMTKKEVMGLFTLEGTMNAILAIALGAVYGVPMFLYFAVNGIPMPSG
                     TSDFGVAIADKIYPAFPPKLIIGTILFIILITAWVSYLPARKIVKMNPTDAIKGKIQ"
     gene            3678..4949
                     /locus_tag="PLI30_07765"
     CDS             3678..4949
                     /locus_tag="PLI30_07765"
                     /inference="COORDINATES: protein motif:HMM:NF014717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HOI79485.1"
                     /translation="MLKFLLKGLMRDRSRSLLPVVVVTLGVMITVFMHAYLSGVLGES
                     LEKTANFSEGHVRVETRAYADNFSQLPNDLALVGVDTLEKSLERLYPEYDWVQRIDFG
                     GLLDVPDSSGNTKMQGTVMGKGVDLLQSDGEATRMELKKLLVKGRFPSKAGEILVSDE
                     LFEKLYLSLGDKVTLISSTMFGEMAMYNFTVAGSLHFGVNVLDRGMMIADIRDVKAAL
                     NMEDAAGTLLGFSKNKLYNDRQAVATKTDFNKRYENDEDKFAPVMSSLSDNGLMGFSY
                     AFIKNIAGFIILVFIFAMSIVLWNAGLISGLRRYGEFGLRMAIGENKSEIFRSLIGEA
                     ALVGIIGTIFGSMLGLSASWLMQEYGINVGSMMNNSSLMISDTLRTQITPFTWFIGFI
                     PGIFSTVIGAMLSGVGIYKRQTANLFKELQN"
     gene            5081..5821
                     /locus_tag="PLI30_07770"
     CDS             5081..5821
                     /locus_tag="PLI30_07770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019538575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein-sorting protein"
                     /protein_id="HOI79486.1"
                     /translation="MNKKTIVIFLSFFSPLLYAQNYPSGNSLLDKIDANMSSETTVMT
                     ASMQVNSARATRTMTLKTWMEGDKKAFTEYLSPAREKGTKMLKLDNQLWLYSPSTDRT
                     IQISGHMLRQSVMGSDLSYEDMMNDTPMQEQYNAVVTGEETINGRKCWVVDMTARISD
                     INYYSRKLWVDQERFVPIKMQLFAKSGKQLKEIALSDIRQIQGRWYPMKTVYKDVLKD
                     GKGTEFTISEISFNQRIPGNVFNKSNLK"
     gene            complement(5827..>6505)
                     /locus_tag="PLI30_07775"
     CDS             complement(5827..>6505)
                     /locus_tag="PLI30_07775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019541066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HOI79487.1"
                     /translation="KTDKIHAIGIGVPSVVDRTQGIVYHVQNIKDWNEVHLKQLLEKE
                     FNIPVHIDNDANCFAKGERIYGLGRDFENFVGITLGTGVGGGIIQKGNLLSDANCGSG
                     EFGEMPYLDGILEQYCGSFFFTNNYGTTGYEVARKVGQNDKKALEIMHEYGKHIAVLV
                     KMIVLTVDPQAIIFGGAIAKSLPLFKESMYEHLNDFPYPNSIKNLKILGSELNHPGIL
                     GAAALCY"
BASE COUNT         1754 a         1314 c         1684 g         1753 t
ORIGIN      
        1 gccgttagag aaaatttcaa acggttgatc ggaataaaaa acgctgccgg ggtagaagtt
       61 tgccagatgg aacggggact ggaagtactt atcggtgtga aaaaagagcc gggattcggc
      121 catatcatta cgtgtggctt aggggggatt tttgttgaaa tccttaaaga tatccagtat
      181 acgttggcac ccgtcacacg gacggaagcg ttgcggatga tccgttcgct gaagtcatat
      241 aagttgatcc agggtgcccg cggaaaagag ggcataagcg aagaagtttt tgccgaagtg
      301 atatgtaaag tatctgacct tctagtactt gttcctgaga ttgaagaaat ggacttgaat
      361 ccgcttatgg ggcgaggaca tcatctttcg gccgtagatg tggtgatcaa aatgtgacgg
      421 aaaatgtcgt ttataaccct gttgttcatt gctgtttttc ctttacacgc acaagaaccg
      481 tcggatagtg ccggttccgt ttctgacgat ggtttgcaca gaattcattt tgttttaaac
      541 ggacagataa ccggatggga aacaacgcaa ctctccaatt cggtgaattg gcagcctggt
      601 tgccgttttt tgcctgtatt gctcggaaac tttagttggg gagaaaattc aaaactcgat
      661 gctgaagcat ctgtccacct ctatgggctg ttaaatttta cagggtggga ttatatcaat
      721 aaggaaggaa aactaaaacc ttaccgggtt tggttacgct attcgggcaa aaactgggag
      781 ctgcgcggcg gtttgcaaaa aatcaatttc ggtacagccc gcatgtttcg tccgctgatg
      841 tggttcgacg ctatggatgt acgtgacccg ttgcaactga cagacggcgt gtacggcatt
      901 ctgggcaagt attttttcga aaacaatgct tccgtctggc tttggtcgct acttggaaac
      961 aaacggccaa aaggttggga gttaatcggt agtgcccgcc gtcgtcccga aatcggtggg
     1021 cgatttgagt tcccggcttt cgccggtgaa atgggcataa gtactcatca tcgaaaaaca
     1081 gatgtgcacc atttgttgcc ggatgttccg gagaatacgc tgctggacga aaaccgtctc
     1141 ggactggatg ggcggtggga cgtgggggtt ggcttgtggt ttgaaaccag ttttacccgg
     1201 ttgcagaaga ataattacaa gatggcactc catcaggatg ccataaacgt agggatggat
     1261 tacactttcc cgttaggaaa cggattgggg ttaacggtgg agtattttcg ttaccatacc
     1321 ggagatggat ttatgcgaaa aggtatgaac ttgaacgtga ttggagaaat gctctcgtat
     1381 ccggtctcgg ttctggataa cctgacggca atgtttttct atgtcggtaa cgatttgaat
     1441 atgtggttga actatatcag ctgggccagg acatacgata aatggagttt ttatctgatg
     1501 ggcttttgga atcctgagat aaacttgccg ttgggcgggc aggcaggggg caagaatctg
     1561 tttatgggaa aaggcatcca attaatggcg agttataatt tttagaaact gtaagacgca
     1621 acatgcggtt tcttacggtg aaaaacttcg ataaagataa aagcactaaa tttatggaca
     1681 gcaaaaacaa cttgattgtt gatgttaacg gactggttaa gaggtttccg ataggctttt
     1741 caggattctt tacggcgttg aagggtattg acttaaccgt atcggaaggt gagtttatgg
     1801 gcctggttgg gccaagcggt tcggggaaaa ctacgttttt gaacattatt ggaacactcg
     1861 ataaaccgtc tgaaggtcgg gtaaacgtgt tggacaagaa cgcagggacc cttactccca
     1921 ggcaagctgc acagctgagg aaggaggaaa tgggttttat ctttcaaagt ttcaacttgc
     1981 tacctgttta cacggttttt gaaaatgtgg agtttccgtt gttgttgctt aacctatctt
     2041 atggggaacg caaaaaacgg gtgatggaga cgttggaatg gctgggattg acttcgaaaa
     2101 taaattcccg accgcctcaa ttatcgggag gggagtgtca acgggtggcg attgcaaggg
     2161 cgatggtaaa acgtccgaaa ctggtattgg ctgacgaacc gactgctaat ctggatgctg
     2221 acaattcgca ccgcattctg aaaaccatgg tgaaactgaa cgaagaactg aaaacctgtt
     2281 tcatttttgc cacgcacgat gaaaaagtga tcagctacct gaagcgaatc gtacatttgg
     2341 aagatggccg tattgtaaaa gatatcagca ggcaaaagtc ggagttacaa taagaaaagt
     2401 gaggtatgaa aatattgaaa ctagtcttcc gaaacctcat tggcaacggg ctgaaaacgt
     2461 ggctgaacgt ttttgtgctg tcgattgcgt tcgtgttgat catttttatg caggcgattt
     2521 tgcagggctg ggataggcag gctatgtccg ataccgtaaa atgggaaatt gccgacgggc
     2581 agtattggaa tgcgaattac gacccgtttg atccctttac actggacagc gctgtgtcga
     2641 ttatcccgaa acagcttgtc aaccaatacg aaaccggaca agttgagcct gttctgatag
     2701 ttgccggcac tatttatccg tcgggccgga gcatgccggt gttgatgaaa ggcatccgct
     2761 ccggacaaca attgctggag cttcccacgt cgttgttgat gaacccgact gacagcgccg
     2821 atatcccggc aattatcgga tcgaatatgg cacaccagtc tggactgacg gaaaacgata
     2881 ttgtaacgct acggtggcgc gatgccaacg gtactttcga agcgcgggat attcgcattg
     2941 ccggcgtatt cagaacattt gttccgacgg tagacgccgg gcagatctgg cttccgcttg
     3001 acatattgca gaagataacg ctgaattctg gtaaagttag ccttcttgtt aaatcgaaag
     3061 aggtgagcgt cgtgccggtt gacggctgga atttcaagga cgtagatgaa ctcactcgac
     3121 cgctgaggga gaccttgcga tcaaaaaccg ttggacaaag cgttttttac gtgatcttcc
     3181 tgttgttggc tttgttggct gttttcgata cacaaacgct ttccatcttc tatcgccagc
     3241 gcgagatcgg cacctttgtg gccctcggaa tgactaaaaa agaagtgatg ggtttgttta
     3301 cgctcgaagg gaccatgaat gctatccttg ccatcgctct cggtgctgtt tacggcgttc
     3361 ccatgtttct ttattttgct gtaaacggta tcccgatgcc atccggcaca agtgatttcg
     3421 gggttgccat agccgacaag atttatccgg catttccccc taaacttatt atcggaacca
     3481 tcctgtttat tattttaatc acggcatggg taagctatct ccctgcccgg aaaattgtaa
     3541 aaatgaatcc gaccgatgcc atcaagggga aaatacaata aagtaaacat ccgatgtcag
     3601 gcatcaggtt tcagaaaatt agccattaga cttctgtcag cgaagcggtc tgatacctgg
     3661 catctaataa aaaaactatg ttaaagtttc tgttaaaagg attaatgcgg gatcgaagcc
     3721 ggagcctctt gccggttgtt gttgtgacac tgggggtgat gattaccgtg ttcatgcatg
     3781 cttatttgag cggtgtgctt ggcgagagtc tggaaaaaac ggctaatttc tccgagggtc
     3841 acgtaagggt ggaaaccagg gcctatgccg ataatttttc gcaattgccc aacgatctgg
     3901 cgcttgtggg tgttgatacg ttggaaaaaa gccttgaaag gctctatccg gaatacgatt
     3961 gggtgcagcg gattgatttc ggtgggttgt tggacgtgcc tgatagttcg ggaaatacga
     4021 aaatgcaagg cacggtgatg ggaaaaggcg tcgatctgct gcaatcggat ggtgaagcca
     4081 cccgtatgga actcaaaaaa ctgctggtga agggacggtt tccttccaaa gccggagaaa
     4141 tactggtcag cgatgaactt tttgaaaaat tgtacctaag tctgggcgac aaggttacac
     4201 tgatttcttc caccatgttt ggcgaaatgg ctatgtacaa ttttacggtg gccggttcgt
     4261 tgcattttgg agtaaatgta ctcgaccggg gaatgatgat tgccgatata cgcgacgtga
     4321 aggcagcctt gaatatggag gatgctgcgg gaaccctact cggtttctct aaaaacaagt
     4381 tgtacaacga tagacaagct gttgcaacaa aaacagattt caacaaacgg tatgaaaacg
     4441 acgaagataa gttcgcaccg gtgatgtctt ccctttcgga caacggcttg atgggtttct
     4501 cttacgcttt tatcaaaaat atcgccggat tcatcattct ggtttttatt tttgccatgt
     4561 ctatcgtact ttggaatgcc ggtttgatta gcggcttgag gagatacggt gagttcggac
     4621 tgcggatggc tattggcgaa aataaatcgg aaattttcag gtcgcttatc ggagaagccg
     4681 cattggtagg aattatcgga actattttcg gctcaatgct gggattatcg gcatcgtggt
     4741 tgatgcagga atacggcatc aacgtgggta gcatgatgaa taactcatcg ttgatgattt
     4801 ccgacacgtt acgaacacag ataactcctt tcacctggtt tatcgggttt atcccaggaa
     4861 ttttttcgac ggttattgga gccatgttgt cgggagtcgg gatttataaa cgacagacgg
     4921 ctaacttatt taaagagtta cagaattaat tggaatgcct ccaaggcgac ctgggcagtc
     4981 agggcaaaat gagagtcgtg cggaaacggc tacctgaaga ctgtgtgaaa ccttgaggca
     5041 gggataaaaa agatttaaca acactgaaga cattcaagca atgaacaaaa aaacgatagt
     5101 aatattcctc tcttttttca gccccttatt gtatgcacaa aattaccctt cgggaaattc
     5161 cctgttggac aagatagatg ccaatatgtc gtcagaaaca actgtcatga cggcttcgat
     5221 gcaggtgaac agtgcacgcg ccacgcgtac gatgacgttg aaaacatgga tggaaggcga
     5281 caagaaggcc tttactgaat atctctcgcc ggcaagggag aaaggaacca aaatgttgaa
     5341 gctcgataat caactgtggc tttactcacc atcgaccgac cgcaccatcc agatttcggg
     5401 tcacatgctg cgtcagtcgg ttatgggaag cgacttgtct tacgaggata tgatgaacga
     5461 tacaccaatg caggagcaat ataatgccgt agttaccgga gaagaaacaa taaacggtag
     5521 aaaatgctgg gtggttgaca tgaccgccag gataagcgat attaattatt actcccggaa
     5581 attatgggtc gatcaggaac ggttcgtccc gatcaagatg caactgtttg ccaaaagcgg
     5641 aaagcaattg aaggagatag ctttatcgga tatacggcag atacaaggac gttggtatcc
     5701 aatgaaaacg gtctacaagg atgtgctgaa agacggaaaa ggaacagagt ttaccataag
     5761 cgaaatttcg ttcaaccaac gtattcccgg gaatgttttc aataaatcga atttgaagta
     5821 gaaccatcag taacacaatg ccgctgctcc cagaattccg ggatggttta gttccgatcc
     5881 cagtattttc aggtttttga tggaattggg gtagggaaag tcgttcaagt gttcgtacat
     5941 cgattcttta aataaaggaa gcgatttggc aatcgcacct ccgaagataa tggcttgcgg
     6001 atcaacggta agaacaatca tttttaccag tacagcaata tgttttccat attcatgcat
     6061 gatttccaac gcttttttat cgttctgccc tactttccgg gcgacttcat atcccgttgt
     6121 accgtagttg tttgtgaaga aaaagcttcc acaatattgc tcgaggatac cgtccaggta
     6181 aggcatttct ccaaattctc ccgatccgca gttggcatcg ctcagcaggt tccctttttg
     6241 tattattcca ccgcccacgc ctgtgcccag agtaattccg acaaaattct caaaatcccg
     6301 gccaagtccg taaatacgtt ctcccttggc aaaacagttg gcgtcgttat cgatgtgtac
     6361 cggtatgttg aattcctttt ctagcagctg cttcaggtgt acctcattcc aatctttgat
     6421 gttttgtacg tggtaaacga tgccttgggt acgatccaca acgctcggga cgccaatccc
     6481 tatggcatgg attttatcgg ttttc
//
LOCUS       DAOSDR010000172         6503 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrimonas sp. isolate AMR_MDS_1826
            NODE_4291_length_6503_cov_4.326768, whole genome shotgun sequence.
ACCESSION   DAOSDR010000172 DAOSDR010000000
VERSION     DAOSDR010000172.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863355
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrimonas sp. (wastewater metagenome)
  ORGANISM  Petrimonas sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Dysgonomonadaceae; Petrimonas.
REFERENCE   1  (bases 1 to 6503)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6503)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:10:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,720
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 35
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6503
                     /organism="Petrimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4291_length_6503_cov_4.326768"
                     /isolate="AMR_MDS_1826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2023866"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..975)
                     /gene="wecB"
                     /locus_tag="PLI30_07780"
     CDS             complement(<1..975)
                     /gene="wecB"
                     /locus_tag="PLI30_07780"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013611236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HOI79488.1"
                     /translation="MQKILTIVGARPQFIKASALSRAFQQLGVPEVLVHAGQHFDANM
                     AEIFFREMEIPWPAYNLGIHSLPHGAMTGRMTEAIEQVLLTEKPSAVVVYGDTNSTLA
                     GALAAVKLHIPVAHVEAGLRGFNLRMPEEINRILTDRISSFLFCPSANAVKNLKEEGF
                     DHFPARVAFFGDVMFDVALHYSQIAVRKSKILAQHQLGGSPFLLATLHRQENTDDPAR
                     LSALLEALNSLAATYRIVLPLHPRTRKIIESAGPKLKFEPLEPVGYFDMVELLKNCSF
                     VLTDSGGLQKEAFFFGKNCLVARDETEWTELTELGYNFLVGADKNRILS"
     gene            1054..2124
                     /locus_tag="PLI30_07785"
     CDS             1054..2124
                     /locus_tag="PLI30_07785"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HOI79489.1"
                     /translation="MKRKKVLLVYPNYSSFVKTDFEILSSEFEVTRYHFQPVKGALRT
                     LRELMKQFVFLLFNLRKFNFVFIWFADTHAFGPVLFGNLLKIKSAVVIGGFDAVSLPE
                     IKYGLYCSNQIRQFLGKYAVKHASYLLPVDESLIENTNYFADPAGKGLPVGVKKFVPG
                     IKGKIIVVPTGYDTDFWQPTKGVVRKKSVVTVAAITNWQRWYLKGCDFLSQIASKMPE
                     TEFYIYGISNAMFEELQKIALPDNLYLNGLVSTEKLPAIYSAHQIYAQLSLSEGLPNV
                     LCEAILCGCIPIGSNVNGIPRIIENKGLIIKNQKKTEAINAILYAQKISDIEIEKNRT
                     LIISQFDKNKRIKALKLLLTEV"
     gene            complement(2098..3138)
                     /locus_tag="PLI30_07790"
     CDS             complement(2098..3138)
                     /locus_tag="PLI30_07790"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HOI79490.1"
                     /translation="MKKILFITQGNLDHASSRIRAVQYFPLLKKNGYHFVWIPRIPSK
                     KKSLFWRFLFFPIIKRINAIRILGSVLIVNYDILFIQRYFLPSWCFRYISFFEKKRVI
                     FDFDDAIYLSDADEKSSVKTKRMICSADHVITSTTVLANYAKQINSNVSVITSPVDIS
                     ALKPGCCSEGGVTVGWIGSEWTSKYLKPLENVFLLLQSEDKVKYLLVGCTEDLLKGIE
                     RKIVPWSLSKEAEYLSQMDIGIMPLESGEFEQGKGGYKLFQYMAACIPVVASPVGINT
                     EIVEPWSNGFLCHSNGEWLESLRILVKDRDLRKKMGQMGRIKVQNYYSLEICTAKLIK
                     VLNELYFSQKQL"
     gene            complement(3147..3782)
                     /locus_tag="PLI30_07795"
     CDS             complement(3147..3782)
                     /locus_tag="PLI30_07795"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF018696.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOI79491.1"
                     /translation="MKKERYYNSFRQEMIQFLPPVIKRSIEFGCGFGNFSAYIKSSYN
                     AETWGVDFSQDAVNESKKVLDNVINAEAFAAMDVLPLDYFDCIICNDFLEHLECPEIF
                     FSLIRKHLTQEACIVASVPNVRYWNNVIHFLVEKDWKYTDQGILDNTHLRFFTEKSLT
                     RFINENRMVIEVMKGINKKKSGKLEILNIMTLGLIRDMRFLQFGFRARFKN"
     gene            complement(3797..4654)
                     /locus_tag="PLI30_07800"
     CDS             complement(3797..4654)
                     /locus_tag="PLI30_07800"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOI79492.1"
                     /translation="MNMRVAGVVVLYNPDFKKLILNICSIISEVEMLFLIDNSDSICI
                     KVDDLCKLSNNIRYIRNNENCGIAKALNQGAIEAIKEGFEWLLTMDQDSYFSDNLFFK
                     TFLSFQDKTNVVIFSPSFRIESQQLSQDNKYVDTDYVITSGNIINLSIWNKLGGFRVD
                     LFIDEVDTEFCFKARKNGFRIVFFEHIYMVHQLGVSYKVNSIFTGKEFLRYEHPPQRY
                     YYMTRNRLIIIREYIFIYPSKCLVKLLHFPRMIYGIIAYETQRKQKIFYILKGVFHFF
                     IGKKGALLM"
     gene            complement(4651..6141)
                     /locus_tag="PLI30_07805"
     CDS             complement(4651..6141)
                     /locus_tag="PLI30_07805"
                     /inference="COORDINATES: protein motif:HMM:NF014051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HOI79493.1"
                     /translation="MGIIIKQSVRGAFWSYLGIAIGYVNVGIIMPQFFDTAQVGLVQL
                     FASVSLIFAQFGTLGFTSVINRLFPLFRDPARQHNGFLFLALTTGLVGFLLSMAVFFI
                     FKPWVVESNIDRSPLLVDYLWWMLPLVLMRILFLLLDNYNKMLYDAVTGTFWMEFIHR
                     IINLFLIVFFALDWISFKCFFSGYILSISIPVIPIIYVLVRRKEFDLAPQPVFFQRNI
                     QKETFKTMVFGFVNGLAGVVLINIDRIFVNQYLSLSEVGIFSVCTLFASFIRVPYNSI
                     AKIATGVIAESWKKNNKSHIQDIFQKAALNQAIFGVLIFVGIVANLHNIFKILPPEYS
                     IGKWVLVIYAAGILINTIYGLAGCITETSKYYRLNTLFLGMVMISQLLLSIVLIKDLG
                     IIGAAISTVSTFILGTLYQAIFQKIYLGIFGLNYKILIVFAIGFLSFGLSALLPEIDL
                     IPDILLRSGIILIFYMTLILRLNISTDLNQYINSLPKTFCFWKNKK"
     gene            complement(6242..>6503)
                     /locus_tag="PLI30_07810"
     CDS             complement(6242..>6503)
                     /locus_tag="PLI30_07810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI79494.1"
                     /translation="VILVRNGAVIPHIKLAQSTSEMDWSKLDLVVYAAGSPALSGWVC
                     LPETNALRSVSVVSRGSGFQLVKDPFQGKVSWRILPSAKRSR"
BASE COUNT         2143 a         1393 c         1097 g         1870 t
ORIGIN      
        1 gctcaggatc cggtttttat cggcgcccac caaaaaatta tagcccagtt cggttaactc
       61 ggtccactcg gtttcgtcgc gggcaaccag gcaatttttc ccgaagaaaa aagcctcctt
      121 ttgcagtcct ccactgtcgg ttagcacaaa cgaacagttt ttgagtaact cgaccatgtc
      181 aaagtacccc accggttcga gcggctcaaa cttcagtttt gggccggcac tttcgataat
      241 cttccgggtt cgcggatgga gcggcagcac aatccggtaa gtcgcagcaa gggagttgag
      301 cgcctccagc agggccgaaa gccgtgccgg atcgtcggta ttctcctgac ggtgcaaggt
      361 cgccaacaga aaagggcttc cgcccaattg atgctgagcc aggattttcg atttacggac
      421 agcaatctgg ctgtaatgaa gcgccacatc aaacataaca tcgccaaaaa acgctacccg
      481 ggcaggaaaa tggtcaaacc cctcctcttt cagatttttc actgcattgg ccgaagggca
      541 gaagagaaac gacgaaatcc ggtcggtgag aatccggttg atctcttcgg gcattctcag
      601 gttaaaaccg cgcaacccgg cctccacgtg ggcgaccgga atgtgaagtt ttacggccgc
      661 cagcgcgccg gcaagcgtcg agttggtatc tccgtaaacc accaccgccg agggtttttc
      721 ggtcagcaaa acctgctcaa tggcttcggt catccggccg gtcatggcgc catgcggcag
      781 cgaatggatt cccaggttgt aagccggcca gggaatttcc atttcacgaa aaaaaatttc
      841 agccatgttg gcgtcgaagt gttgccccgc gtgaaccaac acttcgggaa ccccaagctg
      901 ctggaatgcc ctgctcaatg ccgaagcttt gataaactgc ggccgcgctc ccacaatggt
      961 taggattttt tgcatgaaaa ccggttttct gaattatctg tcaaatttag ctaaatttat
     1021 cgcctgcaat ctttgctgtc tgtcgaaatg gggatgaaac gcaaaaaggt actgctggtc
     1081 tatccaaatt attccagttt tgtaaaaact gactttgaga ttctgtcgtc ggaatttgaa
     1141 gtcacccgct atcatttcca accggtgaaa ggggcactcc gcacgctgcg cgagctgatg
     1201 aaacaatttg tgttcctgct ttttaatctc cgcaagttca actttgtttt catctggttt
     1261 gccgacacgc acgcttttgg gcctgttctg ttcggcaatc ttctgaaaat taaaagcgct
     1321 gtagtcatcg gtggttttga tgccgtttcg ttgccggaaa tcaaatacgg actttattgc
     1381 tcgaaccaga tcaggcaatt tctgggaaaa tacgccgtta aacacgcctc ttatctactt
     1441 ccggtcgatg aatctctcat tgaaaacacc aactattttg cggacccggc aggaaagggt
     1501 ttaccggtag gtgtcaaaaa atttgtgccc ggtataaaag gaaaaataat tgtcgttcct
     1561 accggttacg acactgactt ttggcaacca accaaaggag tggtcagaaa aaagtcggtg
     1621 gtaacggtcg cagccataac caactggcaa agatggtacc tgaaaggttg tgattttctt
     1681 tctcagatag ccagcaaaat gcctgagact gaattttata tttatggaat cagtaacgcc
     1741 atgtttgagg aactacaaaa aatagctctt cccgacaacc tttatttaaa cgggctggtt
     1801 tcaactgaaa aacttcctgc aatttattcg gctcatcaga tttacgccca gctatcatta
     1861 agcgaaggac ttcccaacgt attgtgtgag gccattctct gcggatgtat tccgattggt
     1921 tccaatgtaa atggaattcc aagaatcata gaaaataaag gactgattat aaaaaaccaa
     1981 aaaaaaacag aagcaattaa tgctatttta tacgcccaga aaatatcaga tattgaaatc
     2041 gaaaaaaata gaactttaat aatttctcaa tttgataaaa acaaacgaat caaagcatta
     2101 aagctgcttt tgactgaagt ataattcatt caataccttt atcaatttag ccgtacatat
     2161 ttcaagacta taataatttt gaactttgat gcgtcccatc tgccccatct tttttctgag
     2221 atctctgtct ttaactagaa tacgaagtga ttccaaccac tctccattac tatgacatag
     2281 aaaaccatta ctccaaggtt caacaatttc agtattgata ccgactggag aagcaactac
     2341 tggtatacat gcggccatat attggaataa tttgtatcca ccctttcctt gctcaaactc
     2401 accagattct aacggcatta tcccaatatc catctgagat agatactctg cttccttcga
     2461 taaagaccaa ggaacgattt ttctttcaat tcctttcaag aggtcttcgg tgcaaccaac
     2521 taatagatat ttaaccttat cttcagactg taataataaa aaaacgtttt ctaaaggttt
     2581 aagatatttt gatgtccatt cactaccaat ccatcctacc gtgacaccac cctcgctaca
     2641 acaccctggc ttcaaggcac ttatatcgac cggtgaagtg atgacactta cattgctatt
     2701 tatctgcttg gcataattcg ctaggacagt agtactagta ataacatgat ctgcactaca
     2761 aatcattctt ttagttttta cacttgattt ttcatcagca tctgacaagt aaatggcatc
     2821 atcaaaatca aaaatgactc tttttttttc aaaaaaacta atatatctga aacaccatga
     2881 gggcaaaaaa taacgctgaa taaacaaaat atcataattc actattaata cagatcctaa
     2941 aatgcgaatt gcattaattc ttttaatgat tgggaaaaat aaaaatctcc aaaataaaga
     3001 ttttttcttg gagggaattc ttggtatcca aacaaaatga tatccgtttt tttttaacaa
     3061 tggaaaatat tgaactgcac gaatccttga actggcgtga tcaagattcc cctgagttat
     3121 aaataatatt tttttcatcc ttcagcttaa tttttaaatc tagccctaaa tccaaattgt
     3181 agaaatcgca tatctctaat taatcccaat gtcattatat ttaaaatttc aagtttcccg
     3241 gatttctttt tattgatccc cttcatcact tcaataacca ttctattctc attaataaat
     3301 cgagtaaggc ttttttctgt aaaaaagcgc aaatgggtgt tatctaaaat tccctgatca
     3361 gtatatttcc aatctttttc cactaaaaaa tgaataacat tattccaata cctcacattg
     3421 gggacagagg ccacgataca tgcttcttgg gtaagatgtt tcctgatcaa tgaaaaaaaa
     3481 atttcaggac attcaagatg ttccaaaaaa tcattacaaa taatgcaatc aaaataatct
     3541 aggggcaaca catccattgc tgcaaatgcc tctgcattta taacattgtc taatactttt
     3601 ttcgactcat tcactgcatc ttgactaaaa tcaacacccc atgtttctgc attatatgat
     3661 gattttatat atgcagaaaa gttaccgaaa ccacatccaa actcaatact tctttttatc
     3721 acaggaggta gaaattgaat catttcctgt ctaaatgaat tataatatct ctccttcttc
     3781 attttatcta ctttagttac atcaataaag cacctttttt acctataaaa aaatgaaaaa
     3841 caccttttaa tatatagaat atcttctgtt ttcgttgagt ttcgtaagca atgattccat
     3901 aaatcattcg agggaaatgc aataatttaa caagacactt agaaggatat atgaaaatat
     3961 attctcttat aattatcagg cgattacggg tcatataata atatctttga ggtggatgct
     4021 cgtatctcaa aaattctttg cctgtaaata tactattgac tttataactt actcccaact
     4081 gatggaccat gtaaatatgt tcaaaaaaaa cgatccgaaa cccatttttc cgagctttga
     4141 aacaaaactc tgtatctact tcatcaataa ataaatccac cctgaatcca ccaagtttat
     4201 tccaaataga aagattaata atatttccag aggtaattac ataatcagta tccacatatt
     4261 tattatcttg agatagttgt tgggattcaa ttctaaagga gggggaaaaa attaccacat
     4321 tagttttatc ttgaaaagac aaaaaggttt taaaaaatag attatctgaa aaataggagt
     4381 cttggtccat cgttaatagc cattcaaatc cttcttttat agcttcaatt gcaccttgat
     4441 tcagtgcttt ggctattccg caattctcat tatttcttat ataccttata ttattagaca
     4501 gcttgcataa atcatcaact ttaatacaaa tagaatctga gttatcaatt aaaaacaaca
     4561 tctcaacctc tgatattatc gaacaaatat ttaatataag ttttttaaaa tcaggattat
     4621 agagcacaac aacaccagca actctcatat tcatttttta tttttccaaa aacaaaatgt
     4681 tttaggcaaa gaatttatgt attgattcaa atccgttgaa atattaagtc tcaaaattaa
     4741 agtcatataa aatatcagaa ttattccact tctaagcaat atatctggaa taagatctat
     4801 ttcaggtaac aaagcactga gaccaaaact taaaaaacct attgcaaaaa caatcaatat
     4861 cttatagttc agcccaaata ttcccaagta aattttctga aaaatagctt ggtacaatgt
     4921 accaaggatg aaagtagaaa ctgtagaaat tgctgcgcct attattccta aatccttaat
     4981 aaggacaatg ctcaacaaca attgcgatat cataaccata cctagaaaca atgtatttaa
     5041 cctgtaatac tttgatgtct cagtgataca accagccaat ccataaattg tattaataag
     5101 tattccggct gcataaatta ctagtaccca tttcccaata ctatactcag gaggtaaaat
     5161 tttaaaaata ttatgaaggt ttgcaacaat tcctacaaaa attaatactc caaaaatagc
     5221 ctgattaaga gcggcttttt ggaaaatatc ttgaatgtgt gatttgttat tttttttcca
     5281 ggattctgca ataactccag tggcaatttt agcaatagaa ttatatggga ctcttataaa
     5341 agatgcaaat aaagtacaaa cgctaaaaat tcccacttca ctcaaagaca aatattggtt
     5401 aacaaagatc ctatctatat taataagaac gacacccgcc aaaccattaa caaatccaaa
     5461 aaccatagtc ttaaaagttt ccttttgaat atttctttga aaaaaaacag gttgtggtgc
     5521 taaatcgaat tctttcctac ggactaaaac ataaataatt ggaattacag gaatacttat
     5581 agatagaatg taacccgaaa aaaaacattt aaagcttatc caatcaagcg caaaaaaaac
     5641 aatcaagaaa aggtttataa ttctatgtat gaactccatc caaaaagtac ccgtaacagc
     5701 atcatacaac attttgttgt aattatcgag gagcagaaag agaatcctca ttaaaacaag
     5761 aggtaacatc caccataggt aatcgaccaa aagtggcgac ctatcaatat tactctctac
     5821 cacccaaggt ttaaaaataa aaaaaacagc cattgaaagt aaaaagccta ccagtcctgt
     5881 cgtcaaggca agaaacaaaa atccgttatg ttgccttgcc ggatcacgga aaagcggaaa
     5941 cagccggttg atcacactgg taaaccccaa tgtcccaaac tgggcaaaaa ttaaagaaac
     6001 agaggcaaaa agttgaacta atcccacttg ggctgtatcg aaaaactggg gcataataat
     6061 ccccacgttc acgtatccga tcgcaattcc cagatacgac cagaacgccc ctctaaccga
     6121 ttgtttgata ataattccca tatccaggga tcaaaactac ggaaaagaac caattttcct
     6181 tttggtaacc tctaaaagtt ctatcccgcg ggaatgacaa aatgccggtt gaaccaaccc
     6241 gttaccggct gcgtttggcc gaagggagaa ttcgccagct cacctttccc tgaaacggat
     6301 ccttcaccag ctgaaagcca cttccccgcg aaaccacgga aaccgatctc agcgcattgg
     6361 tttcggggag gcagacccag ccgctgagcg ctggcgaacc cgccgcgtaa accaccaggt
     6421 cgagttttga ccagtccatt tcactggttg actgggccag cttgatatgc gggatgacgg
     6481 ctccgttgcg gaccagaatc acc
//
LOCUS       DAOSDR010000173         6472 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrimonas sp. isolate AMR_MDS_1826
            NODE_4322_length_6472_cov_2.490260, whole genome shotgun sequence.
ACCESSION   DAOSDR010000173 DAOSDR010000000
VERSION     DAOSDR010000173.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863355
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrimonas sp. (wastewater metagenome)
  ORGANISM  Petrimonas sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Dysgonomonadaceae; Petrimonas.
REFERENCE   1  (bases 1 to 6472)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6472)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:10:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,720
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 35
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6472
                     /organism="Petrimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4322_length_6472_cov_2.490260"
                     /isolate="AMR_MDS_1826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2023866"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..486)
                     /locus_tag="PLI30_07815"
     CDS             complement(<1..486)
                     /locus_tag="PLI30_07815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018667596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOI79495.1"
                     /translation="MDAARKNAYLYPLMIIGGLFFIFGFVTWAIAVLIPYLQIACELT
                     SIQAMLVSSAFYISYFVMAIPSGYLLKRIGYKNALTFGLVSMAVGSLLFIPAAITRIY
                     AIFLLGLFVQGLGLSILQTASNPYVTILGPRESGARRMSIMGICNGVAGILGPLILGS
                     IV"
     gene            complement(486..1313)
                     /locus_tag="PLI30_07820"
     CDS             complement(486..1313)
                     /locus_tag="PLI30_07820"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019537674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016835 - carbon-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046348 - amino sugar catabolic process
                     [Evidence IEA];
                     GO_process: GO:0097173 - N-acetylmuramic acid catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HOI79496.1"
                     /translation="MEKITEQPSLYNDLEKKSVEELTSGLISEYKKIALAVEAAKPQL
                     DRLLNAVVEKVKAGGRMIYLGAGTGGRLSVLDVLELPTTFGMEDDTICAVLAGGIDKL
                     ILALEEKEDDTEESWQMLTEKGVSSRDIVVGISASGTTPFVLSGLKNCRENGITTCCI
                     VNNPGSPIAEVSDYPVEVITGPEFVTGSTRMKAGTSQKMVFDMISTTVWIQMGRVLDN
                     RMVNVQLINRKITDRAVRILMDKSDIRDYDQAHTFLLEQGSVANALKTLHQNGGIRK"
     gene            complement(1377..3311)
                     /locus_tag="PLI30_07825"
     CDS             complement(1377..3311)
                     /locus_tag="PLI30_07825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI79497.1"
                     /translation="MRIKTVFISQLIVLFLYPCFSQDSYRVGFSSTSLEPDDQFVSLT
                     LAGYAAPWEGRFTLHWKEKGTMPAYLGITGGESHLFFVDGQSLYRSSSKNTSRWEKIG
                     DATGIRQIAAGTNTIYGVDSEGQLKKSDLSRKKLRWKNLGHWDQPVHAIAVAGNKLYL
                     ADKEGLFHVADLKARKLKWEKASFFPLEDVISLAGDTDRLLALTREGVLYQQGGTYQQ
                     GKWIKIGYKNGVTVHEDIKALALAGHQFYGIDSSNRLFQGEHRSRQELSARALSIATA
                     DKTVIVVALDLTGINDSFTNMVKNELYKKRALPHSAVFINSSHTHFAPVTQNWPTWQE
                     SNRIADSTYLYTVVREAIVKAVEESIDNAKPAELFIGRGSAQLGYNRSLRDHPEIYDN
                     AVDVLCFRYLHDQSEGCLFIAACHPVFSSPEDRFTLSANFPGVARKVIEEKSGITRTL
                     FLQGTAGDINPTDNSEYTTGEKLGNEVMAVLNRPMEKIGGPLTFFLDSVVFDVPVKSR
                     DEILAYTGDEKINANAMLAERNQTWGEIMLDYLKRGTKQFPMPVYVHTLNVGNWKLVG
                     FSRETTTPYSLHVKKMWPGQMVSVTGYTNDVSSYLPTHLHIEKRNYEGMDSFYWYGMP
                     DTYPWNVEEKILTEIKNNNR"
     gene            complement(3383..4738)
                     /locus_tag="PLI30_07830"
     CDS             complement(3383..4738)
                     /locus_tag="PLI30_07830"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019537676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HOI79498.1"
                     /translation="MTTKKINRRGFIKSSSMIGMGAALGLSGLPYSMQSQTRRSAILG
                     GKRSFNGTWHGWPVVGTPEEEELLKVLRSGKWCRLGSPTAPRFDDEYRKMTGSGHALA
                     TSSGTTALTTMLGALDIGPGDEVILPPYTFIATYNVIVQNYALPIFVDTDIESFQIDA
                     SKIESAITSQTRALMPVHIGGSPFDVDTVMKVASKHNLPVIEDACQAHFAEWKGKKVG
                     NFGLGGAFSFQASKNLNSGEGGAIITNDKGFYNKCYSFHHQGQGSDAASLEPGSGTRG
                     SNLRMTEFQAAILLAQMTRIPEQIKQRSENAAYLSGLLSEIPGIKPAKLYEGTTRSAF
                     HLYMFRYNKEAFAGMDRSTFVRALNAEGIPCSTGYTSLTKEAYVHNLAKNKHYLKIYG
                     ERGMKQWLEQIACPVNDKLCEEEALWFTQTMLLGDRKNMDMIADAIRKISREAKAISK
                     L"
     gene            complement(4775..5530)
                     /locus_tag="PLI30_07835"
     CDS             complement(4775..5530)
                     /locus_tag="PLI30_07835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019537686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HOI79499.1"
                     /translation="MLKEERFKIIMKQINLHNKVLSVDLSKLLNVSEDTIRRDLKELA
                     DGGHIVRVHGGAISKSLVRPFIADVNVYSIEEKRIIAAKAVKLIENNMTLLFEGGTTI
                     FELTKVIPENLNLTIFTISPQIAISLSEYKNIKVFTIGGELKKDHNIHVGAHTINELH
                     RIRFDLCFMGVNAISVERGMTDIDMEVVEVNKAMFEVSEKNVLLTIAEKLNLSQRHQV
                     SDLSDIDFLITELDCNNPFLDGFRNQYSHINIH"
     gene            complement(5831..>6472)
                     /locus_tag="PLI30_07840"
     CDS             complement(5831..>6472)
                     /locus_tag="PLI30_07840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI79500.1"
                     /translation="RTGIANPDFPGIARFLRQLEVPDVLHLHFNGAGGNIAAGKYNDG
                     SKINRWILAQRLADGMRRAWSATEKEAVAAVSVEWLVQPVRLRPSDKSLEEVNKWEKT
                     KEKEALPGIVSRVAYIQRYRDGGETDITCLKIGKARIIGLPGECFVEFQLFAKKIRPD
                     LFVAVAAYGDYGPGYIAPREAYKQGGYEVEVSGISPEASDTLMQAIESLLTQK"
BASE COUNT         1639 a         1539 c         1430 g         1864 t
ORIGIN      
        1 gacaatggat cccaagatga ggggccccag aataccggca acaccattgc agatgcccat
       61 gatgctcatc ctgcgggctc cgctttcccg gggaccaaga atggtcacgt aaggattgga
      121 tgcggtttgc agtatggaaa ggcctaatcc ctggacaaac agtccgagga ggaagatggc
      181 ataaatacgg gttatcgctg ccggaataaa cagcaatgaa ccaacagcca tgcttacaag
      241 accaaatgtg agtgcattct tgtagccgat ccttttcagt agataccctg aagggatcgc
      301 catcacaaaa taggaaatat agaaggctga ggagaccaac atggcctgga ttgatgtcaa
      361 ttcacaggca atctgcagat aagggatcaa tacggcgata gcccaggtga cgaagccaaa
      421 aataaaaaat aatcccccga taatcattaa agggtagaga taagcgttct tgcgtgcagc
      481 atccatcact ttctaatccc tccgttttgg tgtaacgttt tcaaggcatt ggctacactg
      541 ccttgttcaa gcaaaaacgt atgtgcctga tcgtagtctc tgatatcgga tttgtccatc
      601 aaaatccgca cagccctgtc ggtaatcttg cggttgatca gctgcacgtt taccattctg
      661 ttgtcgagca ccctgcccat ttggatccag accgtagtgc tgatcatgtc aaacaccatc
      721 ttctgtgaag tgcctgcttt catcctggtg ctgccggtca cgaactcggg gccggtgata
      781 acttcaaccg gatagtcaga gacttccgcg atcggtgagc cggggttatt gacaatgcag
      841 caggtggtga ttccgttttc tctgcaattc ttaaggccgc tcaatacaaa aggtgtagtt
      901 ccgctggccg agatgcccac cacgatgtca cgggatgaga cacccttttc ggtgagcatc
      961 tgccatgact cttcggtatc gtcttctttc tcttccagcg ccagtatcag tttgtctata
     1021 ccaccagcca gcacagcgca gatggtatca tcttccatac caaaggttgt gggtagttcc
     1081 agtacatcaa gcactgaaag acgtccacct gtgccagctc ccagatagat catccgtcca
     1141 cctgctttca ctttctccac aacagcattc aaaagcctgt cgagctgcgg cttggcagcc
     1201 tcaactgcca gtgcaatttt tttatattca gaaatcaatc cagatgtcag ttcttctaca
     1261 cttttcttct ccagatcgtt gtataacgag ggttgttcgg taattttttc catatttgtc
     1321 ttgatgagta aaataagttg tcctgttcgt ttatctatat taaatgtgtg aaatttttat
     1381 cggttgttat ttttaatttc agtaaggatc ttttcttcta cattccacgg ataagtatcg
     1441 ggcattccat accagtaaaa ggaatccatc ccttcataat tccgcttttc gatatgtaag
     1501 tgagtaggta gataactgga aacatcgttt gtataaccgg ttacagagac catttggcct
     1561 ggccacatct tctttacatg caaactgtag ggtgtagttg tttcccgcga gaagcccacc
     1621 agtttccagt tcccgacatt cagtgtatgc acatagaccg gcatcggaaa ttgcttcgtt
     1681 ccccgtttca ggtaatccag catgatctca ccccaggtct ggttcctttc ggcaagcatg
     1741 gcgttggcat tgattttttc atcccctgta taggccagga tctcatccct gcttttcacc
     1801 ggtacatcaa aaacaacgct gtcgagaaaa aaggtgagag gcccaccgat cttttccatt
     1861 ggacgattca gcacagccat cacttcgttg cccaactttt cacccgtcgt atattctgaa
     1921 ttgtctgtcg gattgatgtc cccggcggta ccctgtaaaa agagagtacg ggtgatgccg
     1981 cttttttctt cgatcacttt tctcgctacc cccgggaagt tggcagaaag tgtaaaacga
     2041 tcctccggtg aactgaatac tggatggcac gcagcaataa agagacatcc ttcgctttga
     2101 tcatgcagat aacggaagca aagtacatcg acggcattat catagatctc cggatgatcc
     2161 ctgaggctcc tgttgtagcc gagctgtgca ctgcctctgc caatgaatag ttcggctggc
     2221 ttagcattgt ctatcgattc ttccaccgcc tttacaattg cctcacgtac cactgtatag
     2281 agataggtgg agtcagcaat ccggttgctc tcctgccagg taggccagtt ttgagtgacc
     2341 ggggcaaagt gtgtatggga agagttgata aatacagccg agtgagggag agcccgtttt
     2401 ttatagagct cattcttgac catgttggta aaggagtcat tgataccggt caggtccaga
     2461 gcgacaacta ttacggtttt atcggcagtg gcaatggaga gtgctcttgc cgagagctcc
     2521 tgcctgcttc ggtgttctcc ctgaaataac ctgttggagc tgtctatccc atagaattgg
     2581 tgtccggcaa gggccagggc cttgatatcc tcatggacag ttacaccgtt tttataaccg
     2641 atcttgatcc atttaccctg ctgataggtg ccaccctgtt gataaagcac cccttcccgg
     2701 gtaagagcaa gcaatctgtc agtatcacca gccagagaaa ttacatcttc aagggggaaa
     2761 aaagaggcct tttcccattt cagtttcctg gctttaaggt ctgcgacatg aaataatccc
     2821 tctttgtctg ccagatatag tttatttcct gcaacagcaa tggcatggac cggttgatcc
     2881 cagtggccca ggtttttcca cctgagcttt ttccgggata aatcactttt tttcagctgg
     2941 ccctccgaat caacaccata gattgtgttc gttcccgcgg ctatttgtcg aataccggtt
     3001 gcatcgccga ttttctccca tcgagaagtg tttttgctac tgctgcggta cagcgattgg
     3061 ccatccacaa aaaagaggtg gctttctcca ccggtaattc ccaaataagc gggcattgtt
     3121 cccttttctt tccagtgcag tgtgaatcgg ccttcccaag gagcagcata gccggcaagg
     3181 gtaagtgaga caaactgatc gtccggctca agacttgtag aggaaaaacc cacccggtag
     3241 gaatcctgag aaaaacaggg gtatagaaac aaaacgatca gttgagaaat aaagactgtt
     3301 tttattctca taaatatacg gtttgaagtg atttattctt gaacctgaaa ctctttataa
     3361 tttgaaatga agtacaggaa aattacaact tgctgattgc tttcgcttca cgggatatct
     3421 tcctgatagc gtcggcaatc atatccatgt tctttctgtc accaagcagc atggtttggg
     3481 taaaccataa cgcttcttct tcgcataact tatcgttcac ggggcaggca atctgttcga
     3541 gccattgttt cattcccctc tccccgtaaa ttttcaggta gtgcttgttc ttcgccagat
     3601 tatgtacata ggcctctttg gtcagggaag tataacctgt cgaacaggga atgccttccg
     3661 catttaatgc ccggacaaat gtggagcgat ccataccggc gaaggcttcc ttgttgtaac
     3721 ggaacatata gagatggaaa gcgctgcggg tggtcccttc atagagcttg gcgggcttga
     3781 tcccgggaat ctcggaaagt aaccctgaga gatatgcagc attttctgat cgttgtttta
     3841 tttgttcagg gatgcgcgtc atctgcgcca gaaggattgc tgcctggaac tccgtcattc
     3901 tcaggtttga accacgggtg cccgagccgg gttcaaggct tgccgcatcg gaaccctgtc
     3961 cctggtggtg aaaactgtag catttgttgt agaaaccctt atcgttggtg atgatcgctc
     4021 ctccttcccc ggaattgagg tttttggaag cctggaaact gaaagcccct cccagtccga
     4081 aattaccgac ttttttccct ttccattcgg caaaatgtgc ttggcaggca tcctcaatga
     4141 cagggagatt atgcttggag gcaaccttca tcacggtatc cacatcaaat ggcgatccgc
     4201 caatatgtac cggcattaat gcacgggttt gagaagtgat cgccgactca atctttgagg
     4261 catcaatctg gaaactttct atatccgtat ccacaaaaat gggcagcgca tagttttgta
     4321 caatcacgtt gtaggtggcg ataaaagtat agggtggcaa gatcacctca tcgccggggc
     4381 cgatatccaa tgctccgagc atcgtcgtta aggccgtggt accactagag gtggcaaggg
     4441 catgtccaga accggtcatt ttccggtatt cgtcgtcaaa ccgtggtgct gtgggactgc
     4501 ccaggcgaca ccattttcca cttcgtaata cttttaacaa ctcttcttct tctggagttc
     4561 caactacagg ccatccgtgc caggtaccgt tgaacgagcg ttttccacca agaattgccg
     4621 acctgcgcgt ctgcgattgc attgagtagg gcaagcctga cagcccaagg gctgctccca
     4681 tacctatcat cgatgagctt ttaataaagc ctcttctgtt gatttttttt gttgtcatgt
     4741 tataatcttt tagttattaa attgatgtgg taaactaatg gatatttatg tggctgtatt
     4801 gattccggaa tccgtcgaga aaaggattat tacaatccag ttcggtgatc aagaaatcga
     4861 tgtcggacaa atctgatacc tgatggcgtt gcgataaatt cagtttttca gcaattgtaa
     4921 gcagtacatt cttttccgat acctcaaaca tggccttgtt cacctcaaca acctccatat
     4981 cgatatctgt cattccccgt tcaacggaga tcgcatttac tcccataaaa caaagatcaa
     5041 atcttattcg atgcaactca ttaatggtgt gagcacctac atgaatgtta tgatcttttt
     5101 ttaattcccc cccaatagta aatactttta tgtttttgta ctccgaaaga gagattgcta
     5161 tctgcggact tatggtaaaa atggtgaggt tgagattttc aggaataacc ttcgtcagtt
     5221 caaaaatagt ggtccctccc tcaaaaagca aggtcatgtt gttttcaatc agttttacag
     5281 cttttgcagc aataatcctc ttttcttcta tcgaataaac attgacgtca gcaataaaag
     5341 gcctgaccag ggatttactg atggcaccgc cgtgaaccct gacaatgtgt ccgccatcag
     5401 ccagttcttt caagtctctt ctgatggtgt cttctgatac gtttaacaat ttgctcagat
     5461 caacagaaag cactttattg tgcaaattga tctgtttcat gataatctta aatcgttctt
     5521 cctttagcat atcctgtcgt ctttgatttc gattaggttt ggtgcagcta caacgcatac
     5581 atttaagtat aactaaaaaa cagttattgc gtttttatgc gcttttacaa tgcaaatata
     5641 ggcataaata agtacttaga agtaggatct taatagaaaa aagtaattta gtaatattta
     5701 actattttcc ggagacagga tttttatact caggcagtac aaaagaatga aaacgatcct
     5761 aaaacgctaa tcgtgattga ttgtgatgga gccgatgatt atatgattga ttccttttgt
     5821 gaattctggg ttacttctgg gttagtaaac tttcgattgc ttgcatcaga gtatcacttg
     5881 cttcggggga gatacccgat acctctactt cataacctcc ctgtttataa gcttcccggg
     5941 gggctatata gcccgggccg taatctccgt aggcagcaac tgctacaaac aaatcgggac
     6001 ggattttttt tgcaaacagc tgaaattcta caaaacactc tccgggtagt ccgattattc
     6061 tggcttttcc gatttttaaa cacgtgatgt cagtttctcc gccatccctg tatcgttgaa
     6121 tatatgcaac ccgggagaca attcctggca gagcctcttt ctccttggtt ttttcccatt
     6181 tgttaacctc ttcaagactc ttgtcagaag gacgcaaacg taccggttga accaaccact
     6241 ctactgaaac tgccgcgacg gcttctttct ctgtggcgct ccaggccctt ctcatgccat
     6301 ccgccagacg ttgagcgaga atccatctgt ttatcttcga gccgtcgttg tattttccag
     6361 cggcaatatt gccacctgct ccgttaaaat gcagatgcaa aacatcaggc acctcaagct
     6421 gtctgagaaa acgggctatt cccggaaaat cggggttggc aataccggtc cg
//
LOCUS       DAOSDR010000174         6471 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrimonas sp. isolate AMR_MDS_1826
            NODE_4323_length_6471_cov_3.385754, whole genome shotgun sequence.
ACCESSION   DAOSDR010000174 DAOSDR010000000
VERSION     DAOSDR010000174.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863355
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrimonas sp. (wastewater metagenome)
  ORGANISM  Petrimonas sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Dysgonomonadaceae; Petrimonas.
REFERENCE   1  (bases 1 to 6471)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6471)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:10:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,720
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 35
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6471
                     /organism="Petrimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4323_length_6471_cov_3.385754"
                     /isolate="AMR_MDS_1826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2023866"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(117..2186)
                     /locus_tag="PLI30_07845"
     CDS             complement(117..2186)
                     /locus_tag="PLI30_07845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019539025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF349 domain-containing protein"
                     /protein_id="HOI79501.1"
                     /translation="MNSNNLENEKLPENREGKAEETIDQLPQEEKVSAEPVETTSGEP
                     VEAEKTVAEPEVAETEPVKDAETEQAPDSELKTEKSVDGEEGEINYDIETPEGVNAET
                     EDAEEEVDENSVSDDDSTELLAIEEIVQKLRNLLASDHPQRREVDEVKNQFYRSLRNE
                     TEAQKAVFLESGGESIDFVVNESEIYAEGKELIQKIKEKRAVILAREDAEKEKNVAKK
                     MAIIDQVRHLAENQSQEDFNKTYQEFRSLQQQWNEIKLVPQTKVNELWKEYQRHVEKF
                     YDLVRINNEFREYDFKKNLELKTELCEAAERLDEEQDVVSAFHQLQNLHQEWRDVGPV
                     SRKDREEIWNRFKAASTLINKKYQSHFESLKGKEEENLAAKTALCETLEAIDYSKLNS
                     VRDWNNKIKEVLDIQKQWREIGYVPKKWNTKIYDRYRAACDFFFRNKNDFYKSMHGEM
                     EENLKKKVALCERAEALKDSQDWKKTTQEMISIQKEWKTIGIVPRKYVDSTWKRFIAA
                     CDYFFEQKKLHTSTQHDEEARNFEKKKEIVEKINMLDTTLTPEQALPLLRELMDEWYG
                     IGHVPFKVKDRAYKEFYDATEAQFDRLNIDKTDRKLDSFRSNISDIARSDNAKGQLLR
                     ERERLMRQYERMKVELQTYENNIGFLSVSSKKGNNLVDDMNQKMKKIKSELELLVKKI
                     AAIDDEL"
     gene            2442..2891
                     /locus_tag="PLI30_07850"
     CDS             2442..2891
                     /locus_tag="PLI30_07850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI79502.1"
                     /translation="MEDLKIVYSLPSKVKITALVAGGFLFALAVGVSISQAIHASLDF
                     LFYVEVAGVVLAALLVFTVTVWQSDFIVEIDNDEFRIKLPKQRINGSILWETVTQLGI
                     GLSYLTLTTTGTNYKIDLGNLKYNDLKRIKAKLIEVCEAKNIPFGNI"
     gene            complement(2909..4222)
                     /gene="der"
                     /locus_tag="PLI30_07855"
     CDS             complement(2909..4222)
                     /gene="der"
                     /locus_tag="PLI30_07855"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008661792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HOI79503.1"
                     /translation="MRNLVAIVGRPNVGKSALFNRLTQSRQAIVTDVSGTTRDRLYGK
                     VNWSGQDFSLVDTGGWVVNSDDIMEDEINKQVQIAVEEADVILFVVDVMNGLTDLDNG
                     VAHLLRRSGKPVVVVANKADNFEIGHQSAEFYTLGLGDPFPISAINGSGTGDLLDHVL
                     SLFKKEGEEEKLADIPKFAVVGRPNAGKSSIVNALVGEERNIVTEIAGTTRDSIYTRY
                     DKFGMDFYLIDTAGIRKKGKVTEDLEYFSVIRSIRAIEEADVCILMIDATRGIESQDL
                     NIFSLIQKNRKGLVVFVNKWDLIEEKDNNLIKNFENIIRERFAPFTDFPIIFGSALTK
                     QRILKVMDLAKEVYQNKKQKVPTHKLNEVMLPIIERTPPPANKGKYIKVKYITQLPNT
                     QVPSFVFFCNLPQWIKEPYKRFIENRMRENWNFTGTPINVFFREK"
     gene            complement(4308..>4498)
                     /locus_tag="PLI30_07860"
     CDS             complement(4308..>4498)
                     /locus_tag="PLI30_07860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018710652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HOI79504.1"
                     /translation="RDTQKGIVIGHKGESLKRLGTLARKDIELFFEKKVFLQLYVKVE
                     KDWRNRDNLLKNFGYKLE"
     assembly_gap    4499..4508
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4509..5139)
                     /gene="era"
                     /locus_tag="PLI30_07865"
     CDS             complement(<4509..5139)
                     /gene="era"
                     /locus_tag="PLI30_07865"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018710652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HOI79505.1"
                     /translation="MNRLVGEKLSIITPKSQTTRHRIIGIVNTPEYQVVYSDTPGVLR
                     PNYKLQEQMLNFSLSALDDADVLLYVTDVVEKTDKNDEFLSKVQRLTLPVLLLINKID
                     QTTQTELEKMVDTWHEMLPDAEIYPISALNNFSIDRVQKRILELLPESPPYFEKDALT
                     DKPARFFVNEIIREKALLLYQKEVPYSIEAIVEEFKEETDIIRIRAIILV"
     gene            5501..>6471
                     /locus_tag="PLI30_07870"
     CDS             5501..>6471
                     /locus_tag="PLI30_07870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI79506.1"
                     /translation="MKKIFFFSFLIYFTSSLCAQNQVLTIHPLKPESGDTVTVTYIGD
                     LAKPDTEISYFFNYMGENRFKNEIIPSEFQNNKITGRFKLPDTVLYVSIKITNGKEFD
                     NNRDKGYGFNVYENGKPKKGTFFTQGAVSLYNKYFFNAKVDTLKAIQLMEKEYQLHPD
                     LKESTLPIYLSTLKLDKSKEAEAVKLATEYYNKILETGKNDNFTERIVETIVGRDHQK
                     RDSLINEVIKKHPKGKAALRKQYIDFYFMAMYLPDSALTRYNSIVENFSDIEPEMRTQ
                     LTHSLITTYRGLLDYDNFEKHISKVNDPSFLAREYNEIAWKMATKDK"
BASE COUNT         1782 a         1380 c         1245 g         2054 t
ORIGIN      
        1 agcagatatt ggcatgtttt catctttcta aaaggcagaa gaaaatgtct gtgaggctat
       61 ctaataagaa aaaggcgacc aaaaaagtga tcgcctaact tttatcataa aaaatattag
      121 agttcatcgt ctatggctgc aatttttttc accagcaact caagctctga ttttattttt
      181 ttcatcttct ggttcatatc gtccaccagg ttgttgcctt ttttcgacga aaccgacaag
      241 aagcctatgt tgttttcgta cgtttgcaat tccactttca tacgctcata ttggcgcatc
      301 agcctttccc gttcacgcaa caattggcct ttggcattat ccgatctggc tatatccgag
      361 atgttcgacc tgaaactgtc taatttccgg tcggttttat cgatattcaa cctgtcgaac
      421 tgagcttcgg tagcatcata gaactctttg taagccctgt ccttaacctt gaaaggcacg
      481 tgtccgatac cataccactc atccattagt tcccttaaca gcggcaacgc ctgttcagga
      541 gtgagggtgg tatccagcat gtttatcttc tccacgatct cttttttctt ttcgaaattt
      601 ctggcctctt catcgtgttg agtggaggtg tggagtttct tttgttcaaa gaaataatca
      661 caagcggcaa taaaccgttt ccacgtgctg tccacatatt tgcggggaac aataccaatg
      721 gttttccact ctttctgaat agaaatcatt tcctgggttg tttttttcca atcctggcta
      781 tcctttaagg cttcggcacg ttcacaaaga gcaactttct ttttcaggtt ttcctccatt
      841 tcgccgtgca tcgatttgta gaaatcgttt ttattacgga aaaagaagtc acaggcagca
      901 cggtaacggt cgtatatttt ggtgttccat ttttttggca catagcctat ctcacgccat
      961 tgcttctgaa tatcaagaac ttccttgatc ttgttgttcc agtccctaac ggagtttaat
     1021 ttcgaataat cgatggcttc aagggtttcg cacaacgcgg ttttagccgc cagattctcc
     1081 tcttctttcc ctttaaggct ttcgaaatga gattgatact ttttattgat tagggtagaa
     1141 gctgctttaa aacggttcca gatttcttcg cggtctttgc gagaaaccgg gcctacatca
     1201 cgccactcct ggtgaagatt ttgcagttga tgaaacgccg aaaccacatc ctgttcttcg
     1261 tcgagccttt cggctgcttc gcaaagttcg gtcttgagtt ccagattttt tttgaagtcg
     1321 tattcacgga actcgttgtt gatgcgcacc aggtcgtaaa atttttccac atggcgttgg
     1381 tattctttcc aaagttcatt cactttggtc tgtggtacaa gtttaatctc gttccactgt
     1441 tgctgaagtg agcggaactc ctgataagtt ttgttaaaat cctcctggct ttgattttcg
     1501 gcaaggtgtc ttacctgatc gataatcgcc atttttttag caacattctt ttccttttcc
     1561 gcatcttcac gggcgagaat tacagcacgt ttttctttta ttttttgaat cagttcctta
     1621 ccttcggcat agatttccga ttcattaaca acaaaatcga tagactcgcc tcccgattcg
     1681 aggaaaacag ctttttgcgc ttcggtttcg ttgcgaagtg aacggtaaaa ttgatttttc
     1741 acctcatcca cttctcttcg ctgaggatgg tcggaagcca gcaaatttct gagtttctgc
     1801 acaatctctt ctatggccaa taactccgtg gaatcatcat ccgaaacgct gttttcatct
     1861 acttcttcct ccgcatcttc agtttccgca ttcacacctt ctggagtttc tatatcgtaa
     1921 ttgatttcac cctcctctcc gtcaacagat ttttcggttt tcagctccga atcaggcgct
     1981 tgttccgttt cggcatcctt taccggctct gtttcagcca cttctggttc cgcaactgtt
     2041 ttttctgcct ctaccggctc tccggaagtg gtttcaacgg gctccgcaga tactttttct
     2101 tcctggggaa gttggtcaat agtttcttca gccttacctt ccctgttttc aggaagtttc
     2161 tcattttcaa gattgttact attcatcaat tcactcttcc tattttaatc ccaaccaaca
     2221 tagttgagat atattgctac ataaacgtct tttggtaaaa taagtttacg ttcaatttgt
     2281 ttaacttatc ctataaatta tatacaaatt aacgaatttt ttttctcata tcatcgattt
     2341 ttcatgttta tttacaaaaa accgcgtaat aattgtattt ttgtaaactg aattatttgt
     2401 tattcagcag ataacattaa attcgataaa taacgtttgt tatggaagac ctgaaaattg
     2461 tttattcgtt accgtcgaaa gtgaagatta ccgcattggt tgccggaggg tttctgtttg
     2521 cgctggctgt tggagtcagc atatcccagg ctatacacgc cagcttggat tttctgtttt
     2581 atgttgaggt agcaggagtt gttttagcgg cactattggt gtttacagta accgtttggc
     2641 aatcggattt tattgtggaa attgataacg atgaatttcg aattaaatta cctaaacaac
     2701 gcataaacgg ttccatcttg tgggaaacgg tcacacaact gggtatcggg ctaagctacc
     2761 tgacactgac tactaccggg accaactaca agatagacct gggtaacctt aaatacaacg
     2821 acttaaaaag gattaaggcc aaactgattg aggtctgcga ggcaaaaaac attcctttcg
     2881 ggaatatttg aggaccatta ttgtgtcatc acttttcccg gaaaaaaaca ttaatcggcg
     2941 ttccggtgaa gttccagttt tcacgcatcc ggttttcgat gaaccgctta taaggctctt
     3001 tgatccattg gggaaggttg caaaaaaaca caaaagaggg aacttgagtg tttggcagtt
     3061 gcgttatata cttaactttt atgtacttcc ctttatttgc cggtggcgga gtcctttcaa
     3121 taataggcag catcacttcg ttaagtttgt gcgtaggcac tttttgtttt ttgttctgat
     3181 atacctcttt tgccaaatcc atcactttca gaatccgttg tttagttaag gctgaaccaa
     3241 aaataatggg gaaatcagtg aacggtgcaa atcgttcgcg aataatgttc tcgaagttct
     3301 ttatcaggtt gttgtctttt tcttcaatta aatcccattt attgacgaac accaccaggc
     3361 ccttccggtt tttctggatc agtgagaata tattcaggtc ctggctttct attcctcgcg
     3421 tggcatcaat cataaggata cagacatcag cctcctcaat ggcacggatg gagcggataa
     3481 ccgagaaata ttccaggtct tccgtcactt ttcctttttt gcgaatccct gcagtatcga
     3541 tgaggtaaaa atccatccca aacttatcat accgggtata aatggagtcc cgggtagtac
     3601 cggcaatctc ggtcacgata ttgcgttctt cacccaccag agcatttacg atcgatgatt
     3661 tcccggcatt gggacggcca acaacagcga acttgggaat atctgctaat ttttcctctt
     3721 caccttcttt cttgaacaac gacaaaacgt gatccaacag atcacccgtt cccgaaccat
     3781 tgatggccga aataggaaaa ggatcaccta gccccagcgt ataaaactct gccgattgat
     3841 gaccgatctc aaaattgtcg gctttgttgg caaccacgac tacaggcttg ccggaacgac
     3901 gcagcaaatg agccacccca ttatccaggt cggtaagccc gttcattacg tcaacaacaa
     3961 aaaggatcac gtcggcttct tccaccgcga tctgtacctg cttgtttatt tcgtcctcca
     4021 ttatatcgtc ggaattgact acccagccgc cggtatcgac cagcgaaaag tcttgtccgc
     4081 tccaattgac ttttccgtac aaacggtcgc gtgtggttcc cgatacatcg gtaacaatag
     4141 cctggcggct ttgtgttaac cggttaaata acgctgattt cccgacgttc gggcgtccaa
     4201 ctatagctac taagtttctc atattctttt aagactatca gacaaaagac attgggcttt
     4261 cagactgata aatttaattt gtaatttgta attgataatt ggaaattcta ttccaacttg
     4321 tacccaaaat tcttcaacag attgtccctg tttcgccagt ctttttctac tttaacgtaa
     4381 agttgcaaga atactttttt ttcaaaaaac agttcgatgt cttttcgcgc cagggtaccc
     4441 agtcttttca gcgactcccc tttatggccg attacgatgc ccttttgtgt atctctctnn
     4501 nnnnnnnncc accaagataa tggcgcggat acggataatg tccgtttctt ccttaaattc
     4561 ttccactatc gcttcaatgg aatagggaac ctctttctga tacagcaaca aagctttttc
     4621 gcggatgatc tcgttaacaa agaaccgggc aggcttatcc gtcagcgcat ctttctcaaa
     4681 gtaaggcggt gactcgggca aaagctctaa aatgcgcttt tgcaccctgt caatactgaa
     4741 gttattgagt gctgatatgg ggtaaatttc agcatccggc aacatctcgt gccacgtatc
     4801 aaccattttt tcaagttccg tttgtgtggt ctggtcaatt ttattgataa gcaataaaac
     4861 cggaagggtc aaccgttgta ccttggagag gaattcatcg tttttatcgg ttttttccac
     4921 tacatcggtc acgtacagca acacatcggc atcatcaagc gcggataacg agaagttaag
     4981 catctgctcc tgtagcttgt agttgggacg aagcacaccg ggcgtatcgg aatacactac
     5041 ctggtattca ggggtgttta cgatgccgat aatgcggtga cgcgtggttt gcgatttggg
     5101 cgttattatg gataactttt cgcctaccag ccggttcatc agcgtggatt ttcccacgtt
     5161 gggatttccc acaatattta cgaatccgga acgatgattt tgttgctgca cttattggac
     5221 actttgggtt tgaggtgcaa agatacgaaa aaaagagata cgtcaattaa agagcaaaga
     5281 atcaagacaa aataacggga atggattgcg acaataaact cgtatgatat cataatcatg
     5341 actaatgagt aattatcaag acaacaaatt agtaaatttt ttacgaaaat atagtaataa
     5401 atgatttatt gttctatatt tgcaaataag tataagtgtt tataaccttg gaacacataa
     5461 agatatagca tttactaatc caacataaaa cataatggct atgaaaaaaa tatttttctt
     5521 ttctttcctg atttatttta ccagcagcct gtgtgcacaa aatcaagtcc tgaccataca
     5581 tccattaaag cctgaatcgg gagatacagt aaccgttaca tacataggag atttagcaaa
     5641 accggatacg gaaatttctt atttttttaa ttatatgggg gagaacaggt ttaaaaatga
     5701 aattattccg tctgaatttc aaaacaataa aattacagga cgttttaaat tgccggacac
     5761 agtgttgtat gtttctataa agattaccaa cggaaaagaa tttgacaata accgtgacaa
     5821 aggttacggt ttcaatgttt atgaaaacgg aaaacctaaa aaaggaactt tttttacaca
     5881 aggagcagta agcctctaca acaaatattt ctttaatgca aaagtggata ccctcaaagc
     5941 cattcaactc atggaaaaag agtaccaatt gcatcccgat ttaaaagaat cgacgttacc
     6001 gatatatttg agcacgttga aactcgataa atcaaaagaa gcggaagcag ttaaattggc
     6061 aactgagtat tacaataaaa ttttggaaac agggaaaaac gacaatttta cggagcgaat
     6121 tgttgaaacc atcgttggca gggatcatca aaaaagagat tctttgatca atgaagtgat
     6181 taaaaaacac cctaaaggaa aagcagcgtt aagaaaacaa tatatagatt tttattttat
     6241 ggcgatgtat ttacccgatt ccgcccttac aagatataat tcaatcgtgg aaaatttcag
     6301 tgatatagaa ccggaaatgc gaacacagct tacccacagt ttgattacta cttatagagg
     6361 tttgttggat tacgataatt tcgaaaaaca tatttccaaa gttaacgatc cttcttttct
     6421 tgccagagaa tataacgaaa ttgcttggaa gatggctaca aaggataaag a
//
LOCUS       DAOSDR010000175         6468 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrimonas sp. isolate AMR_MDS_1826
            NODE_4327_length_6468_cov_4.368470, whole genome shotgun sequence.
ACCESSION   DAOSDR010000175 DAOSDR010000000
VERSION     DAOSDR010000175.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863355
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrimonas sp. (wastewater metagenome)
  ORGANISM  Petrimonas sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Dysgonomonadaceae; Petrimonas.
REFERENCE   1  (bases 1 to 6468)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6468)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:10:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,720
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 35
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6468
                     /organism="Petrimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4327_length_6468_cov_4.368470"
                     /isolate="AMR_MDS_1826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2023866"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..345
                     /locus_tag="PLI30_07875"
     CDS             <1..345
                     /locus_tag="PLI30_07875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011966852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HOI79507.1"
                     /translation="SSTMVQLNESLFSWVIENLTKNAVDAMGGEGIITYSMGEKGRHC
                     FLDISDTGKGVSTSKFNRIFQPGFTTKERGWGLGLSLAKRIVENYHEGKIFVKQSEIG
                     KGTTFRILLRKG"
     gene            complement(320..991)
                     /locus_tag="PLI30_07880"
     CDS             complement(320..991)
                     /locus_tag="PLI30_07880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007559752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase beta subunit-related
                     protein"
                     /protein_id="HOI79508.1"
                     /translation="MSEIKITVSDEIFRACPEFCFSAIICRVKNSPHNEKLWKEVEVF
                     STDFRARYKMEDINKRKAIFATRQVYKNLGKDPNRYRPSAEALCRRIIKGINLYPINT
                     LVDLINFVSLKTGYSIGGFDYDKIQGNLVLGIGTAGEKFEAIGRGLLNIEGLPVYRDE
                     VGGIGTPTSDEERTKITGETTHLLMIINGYSGREGLEEATDFSVELLKKYAGAEEIIL
                     SSTKF"
     gene            1221..1586
                     /locus_tag="PLI30_07885"
     CDS             1221..1586
                     /locus_tag="PLI30_07885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI79509.1"
                     /translation="MKNNLQKVVKNESNDYICVSFCFSMTEEKRKLLTDLEFRVRQVM
                     FMCDTLKEENIRLKSDLQVIQQQLVEKTEQLNQLKTKYDSLKTARTITAASVDVRVAK
                     NKLSKLVREVDKCINLLNS"
     gene            1605..1904
                     /locus_tag="PLI30_07890"
     CDS             1605..1904
                     /locus_tag="PLI30_07890"
                     /inference="COORDINATES: protein motif:HMM:NF017016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HOI79510.1"
                     /translation="MGDEKFLLTLEIDGRKYPLKIKQSEEEAFRKAAKTIHNKINQYR
                     VKFGGNPNLTVQDFMAMTAIQALVENFSIESKNNTKPYEDTISTLIKELDLFLKK"
     gene            2087..3616
                     /gene="rny"
                     /locus_tag="PLI30_07895"
     CDS             2087..3616
                     /gene="rny"
                     /locus_tag="PLI30_07895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006798620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HOI79511.1"
                     /translation="MEIVVGVIGIVIGAVIAWYLTGKAANSRAQNILSDAEKDAEVIR
                     KNKLLEAKEEIISMKAEAEKQANARTSKIQITENRLKQREMSLNQRQEELGKKGNEID
                     TLRVNLENQQELLDKKSAELEKVYRQSVEQLETISGLSADEARERLVESVKEEAKTNA
                     QSYINEIMEEAKMTANKEAKRIVVQSIQRVATETAIENAVTVFHIDSDEVKGRIIGRE
                     GRNIRALEAATGVEIVVDDTPEAIVLSGFDPVRREIARLSLHQLVADGRIHPARIEEV
                     VSKVKKQIEEEIVETGKRTVIDLGIHGMHPELIRLIGKMKYRSSYGQNLLQHSRETAN
                     LCAIMAAELGLNPKKARRAGLLHDIGKVPDDEPELPHAMIGMKLAEKYKEKPDICNAI
                     GSHHDEVEMTTLLAPIVQVCDAISGARPGARHEIVEAYMKRLNDLENLALSYPGVVKT
                     YAIQAGRELRVIVGADKLDDQDTEKLSAEIAKKIQTDMTYPGQVKITVIRETRAVSYA
                     K"
     gene            complement(3694..4998)
                     /locus_tag="PLI30_07900"
     CDS             complement(3694..4998)
                     /locus_tag="PLI30_07900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI79512.1"
                     /translation="MKKLINTILIALVATLLTSCMALQGGAYAQNGYDRDYGYYDDDD
                     YYGDDDYYYGDNNYDNRDGVSIQVFYNTLRPHGRWMRHSSYGEIWIPRVGRNFHPYAT
                     NGYWVMTNYGNTWVSDFSWGWAPFHYGRWFYDDYYGWAWIPGYEWAPAWVTWRTGGGY
                     YGWAPMGPRMGINIHINLPINYWVFLPNRYMYHRNMHRYYPGRNFHHNIYNKTTIINN
                     TYVYNNNRYYSGPTANEYRSTTGRSVNVRTLETNGRSGRTAVNDRSVSVYTPGNSRSG
                     GGGNVSRSGNTSPATRGTVNSGARSSEVNRNATTRTAGTSNREAINNRPDNNRNTPAR
                     SSNSTREINNSRSSNGGSSRTVAPQQTSKSRNANVSRSSSSSSSSRSNEATVRSQRSS
                     GSNSRSNAAVRSQSSSSSRNSGSVASTRDNENSSTRSSSGRR"
     gene            complement(5155..6294)
                     /locus_tag="PLI30_07905"
     CDS             complement(5155..6294)
                     /locus_tag="PLI30_07905"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HOI79513.1"
                     /translation="MHYLFLIAGFNAIFFALLLFQKKMARHDKILFCWLIYLGFYTGF
                     YGLFSHKLFTAYHLLSASFVSLLMLNGPFLYLYISSLIQHSFRLNTGRLLHFLPFLLF
                     NLYLLIASFFPGISEGIRLDHVEHASKPPFLFNLFLTLTVLSGPVYFLSSIQLFKKLD
                     INIFNNFSSDTNVNLNWLRKLVYSFGIVWTMLMVTTTIHHIFHLFSLAFCTDGISLSL
                     SLFVILIGYFGLKQKEIFPQEVIEEKNTYVTADKNKEKYAGIPMTEEELNDCAEKISH
                     FVETEKAYLNPDLSLPQLAETLDVPSHHLSRVINDQFGVNFFNFINRYRIEEVKAKIG
                     NPEFQNLSILGIAYDSGFNSKSAFNRVFKKMEDMTPSEYRKQIPK"
     gene            complement(6369..>6468)
                     /locus_tag="PLI30_07910"
     CDS             complement(6369..>6468)
                     /locus_tag="PLI30_07910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019537857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="electron transport complex subunit RsxA"
                     /protein_id="HOI79514.1"
                     /translation="PKAMQGIPIALITAGIIAMAFMGFSGIDQVFK"
BASE COUNT         1867 a         1279 c         1516 g         1806 t
ORIGIN      
        1 tcttcaacaa tggtacaact gaatgaatcg ttgttcagtt gggtaatcga gaacctgacc
       61 aaaaacgcgg ttgatgcaat gggaggagaa ggcattatta cttattcaat gggcgaaaaa
      121 gggaggcatt gctttcttga tatctccgac acgggaaaag gcgtttcaac atcgaaattc
      181 aaccgtattt tccagcccgg attcaccacc aaggaacggg gatggggatt agggttatcc
      241 ctggccaaac gtatagtgga gaactatcac gagggaaaaa tttttgttaa gcagtcagaa
      301 atcggaaaag ggaccacctt tagaattttg ttgaggaaag gataatttct tccgcccccg
      361 catatttttt caacaactcc actgaaaagt ctgttgcttc ctccaggcct tctcttccgg
      421 aatatccgtt tataatcatc aacaggtggg ttgtttcccc ggttattttt gtgcgttctt
      481 cgtcgctggt aggtgtgcct attcccccaa cttcatcccg gtagacaggc aatccttcga
      541 tgtttaacaa accacggcca atagcttcaa atttttctcc ggctgttcca atgcccaaaa
      601 ccagattacc ctgaatttta tcataatcaa accctccgat ggaatatccg gttttcagcg
      661 aaacaaagtt tatcaggtct accaacgtat tgatcggata taaatttata cctttaatta
      721 ttcgtctgca caacgcttct gccgatggcc ggtaccggtt aggatctttt cccagatttt
      781 tatacacctg tcgcgtggca aaaattgctt ttcttttgtt tatatcttcc attttatacc
      841 ttgcccggaa atctgtcgaa aatacctcca cctctttcca taatttttcg ttgtgagggg
      901 aattttttac cctgcagatt atggcagaga agcaaaactc gggacaagcc cggaaaattt
      961 cgtcgctgac ggttatcttg atctcgctca ttcttattta ttgtttaatt ttcgtatgca
     1021 ttagctgctg cccctcatac aactaaatat gagatatctg gatgttttat gggttgcccg
     1081 ttagatgaga caggcataat gccggcccct agttaccgca gtgccaggca ataaatataa
     1141 acctttttga aggtcaatga atcacaaatt tacaaaatct ccaccaaact tttcagtatt
     1201 tagcgcaagg gaatgaattt atgaaaaata acttacaaaa agttgtaaaa aatgagagca
     1261 atgactatat ttgcgtgagt ttttgttttt ctatgactga agagaaaaga aagttgttga
     1321 ctgatctgga gtttcgtgtg agacaggtga tgttcatgtg cgacactctt aaagaagaga
     1381 atattcgact aaaatctgat ttacaggtta ttcaacaaca gttagttgaa aaaacagagc
     1441 aacttaatca gttgaaaaca aaatacgata gtttaaaaac ggcaagaacc attactgcgg
     1501 catcggtgga tgtgagagtt gccaaaaaca agctgtccaa attagtgcga gaagttgata
     1561 aatgcataaa tttattgaac agttaaaaac gggaaattta tgctatgggc gacgaaaagt
     1621 ttttattaac gttggaaatt gacggccgga agtatccact gaagatcaaa caatcggaag
     1681 aggaagcttt cagaaaagct gccaagacaa tacacaataa gatcaatcag tacagggtta
     1741 agtttggagg aaatcccaat ttaaccgtac aagactttat ggctatgact gccatacagg
     1801 cattggtgga gaatttttct attgaaagta agaataatac gaaaccctat gaggatacaa
     1861 tcagtacgct gataaaagaa ctggatcttt ttctaaagaa atagaacaga atcctgattg
     1921 tcagggccaa ctctcatcgc acttttttac ggatgatttt gatcaattcg tagatagtgc
     1981 gatttttttt aacaagattc aacataaaaa taaagtagaa ccatctcttg tgtttgattg
     2041 tataaattga aggcagggat tttattaatc attaataatc agaaatatgg aaatagttgt
     2101 aggagttatc ggcatagtta tcggagcagt tattgcctgg tacttaaccg gtaaggcagc
     2161 aaattcgcgc gctcagaata ttttgagtga tgctgaaaaa gatgctgagg taatcaggaa
     2221 aaataaatta ctggaagcta aagaggaaat tatttcgatg aaggccgaag ctgaaaagca
     2281 ggccaatgca cgtacatcga aaatacaaat taccgaaaac cggttgaaac aacgagagat
     2341 gtcgttgaat caacggcagg aagagctggg taaaaaagga aacgagattg acactcttcg
     2401 tgttaatctg gagaatcagc aagaactgtt ggacaagaaa agtgctgagc tggagaaagt
     2461 ttatcgccag tctgttgaac agttggaaac aatttcggga ctttcggccg atgaagccag
     2521 ggagcgtttg gttgaatctg tcaaggaaga ggccaagacc aacgctcaat cgtacatcaa
     2581 cgagattatg gaagaggcta aaatgacggc aaacaaagaa gctaaacgta ttgttgttca
     2641 aagtatccag cgtgtggcga cagaaaccgc aattgagaat gctgttacgg tctttcatat
     2701 cgattccgat gaagtaaaag ggcgtattat cgggcgtgag ggacgaaata tccgggcgtt
     2761 ggaagccgca acgggggtgg aaattgttgt ggacgatact cccgaggcga ttgttttatc
     2821 gggttttgac cctgttcgtc gtgaaattgc ccgcctgtcg ctccatcaac ttgttgcaga
     2881 tggacgtatt catcctgcaa ggatcgagga ggtggtatct aaagtgaaga aacagattga
     2941 ggaagagatc gttgaaaccg gtaagcgtac ggttattgat ttgggaatcc atgggatgca
     3001 tcccgagttg atccgtttga tcgggaaaat gaaataccgt tcctcctacg gacaaaatct
     3061 gcttcaacac tctcgtgaga cggctaacct ttgcgccatt atggctgctg aactgggatt
     3121 gaatcccaaa aaagccagac gtgccggatt actgcacgat attggcaagg tgccggatga
     3181 tgagccggaa ctgccacacg ctatgattgg aatgaaactg gcggagaaat acaaggaaaa
     3241 acccgatata tgcaacgcta tcggctcgca ccacgatgag gtggagatga ctactctcct
     3301 ggcaccgatc gttcaggttt gtgacgctat ttcgggggcg cgtcccggtg caaggcacga
     3361 aatagtggaa gcgtacatga aacgactgaa cgacctggaa aacctcgcgc tttcgtatcc
     3421 aggtgtggtg aaaacttatg ctattcaggc cggccgggaa ttacgggtta ttgttggagc
     3481 agacaagctc gatgatcagg atacggaaaa attatccgca gaaattgcca agaaaataca
     3541 aaccgatatg acttatcccg gtcaggtgaa aattaccgtg atccgtgaaa caagagcggt
     3601 gagttatgcg aaatagaacg gtttagcgtt accgctaaac actacgaaag ccgctaattt
     3661 tagcggcttt cgttatttat atgttgagac ctgctatctg cgtcctgaag aagacctggt
     3721 agagctgttt tcattgtctc tcgttgatgc tacagacccg ctgttccttg agctggatga
     3781 gctttgcgat ctcactgcgg cgttacttct tgagttcgag ccggaagagc gctgtgacct
     3841 tactgttgct tcattgcttc tggaagaaga ggacgaactg ctgcttctgc ttacgttggc
     3901 attccggctt ttggatgttt gctgaggtgc tacagttctc gaagaaccac cgtttgatga
     3961 gcgtgaattg tttatttcgc gggttgagtt gctgcttcgg gccggagtat tccggttgtt
     4021 gtccgggcgg ttgtttattg cttcacgatt tgaagttcct gcggtccttg tggttgcgtt
     4081 tctgtttact tcgctgctgc gtgcaccgga gttaaccgtt cctcttgttg ccgggcttgt
     4141 gttccccgat ctcgaaacat ttccgcctcc gccgctcctc gagttccccg gggtgtatac
     4201 gcttacagat ctgtcgttaa ccgctgtcct gcctgaacgt ccattcgttt ccaatgtgcg
     4261 tacattcacg cttcttccgg tagtactgcg gtattcgttg gcggtggggc cgctatagta
     4321 gcggttgttg ttgtaaacat aggtgttgtt tatgatggtg gttttgttgt aaatgttgtg
     4381 gtggaaattt cttccgggat agtatctgtg catgtttcta tgatacatat acctgttcgg
     4441 taagaaaacc cagtagttta taggcagatt gatgtgtatg ttgattccca tcctgggacc
     4501 catgggagcc catccgtagt atccgccgcc agttctccaa gttacccagg caggtgccca
     4561 ctcatatccg ggaatccaag cccatccgta gtagtcatcg tagaaccacc gtccgtagtg
     4621 gaaaggtgcc cagccccagg aaaaatcgga tacccaggtg tttccgtagt tggtcatcac
     4681 ccaatatcca ttggtcgcat aggggtggaa attgcgtccc actcgtggta tccagatctc
     4741 accgtaactg ctgtgtctca tccatctgcc gtgaggtcgc agggtattat aaaaaacctg
     4801 aatgcttact ccgtcccggt tatcgtagtt gttatcaccg taataatagt catcatcacc
     4861 gtaatagtca tcatcgtcgt aataaccgta atccctgtcg taaccgtttt gagcataagc
     4921 cccgccttga agagccatgc aggatgtaag taatgttgct acaagggcga ttaagatggt
     4981 gtttattaac tttttcatat ctgtaagtat taatgggtga aacatcgttt tgtttacctt
     5041 taagacaaac aatacgacgt atggtttaac gattggcatt tattttccgc aaaggcaatg
     5101 gagcatccta ttatagcaat ggtttttttg tgcgttatgt taatcggcct gatgctattt
     5161 cggtatttgc tttctatatt ccgatggcgt catgtcttcc attttcttga aaaccctatt
     5221 gaaggccgat ttggagttga atccactatc gtaggcaatt ccaagtatgg ataaattttg
     5281 aaattcagga ttgcctattt tagcttttac ttcttctatc cggtacctat tgataaagtt
     5341 gaaaaaattt acaccgaatt ggtcgtttat tacccgtgag aggtggtgtg aaggcacatc
     5401 aagtgtttct gccaattgag gaagtgacag gtcgggattg aggtacgctt tttccgtttc
     5461 cacaaaatga ctgatctttt ctgcacaatc gttcaattcc tcttcggtca tagggatgcc
     5521 tgcatatttt tccttgtttt tatcggcggt tacgtaggtg tttttctctt cgataacttc
     5581 ctgcggaaag atttcctttt gctttaatcc gaaatatccg ataagaataa caaaaaggga
     5641 caaagaaaga gaaatgccat cggtacagaa tgccagtgaa aacaggtgaa agatgtgatg
     5701 aatggttgtt gtaaccatca gcatcgtcca gacaattccg aaagagtaaa caagtttccg
     5761 gagccaattg aggttgacgt ttgtgtcgga agagaagttg ttgaagatat taatatccag
     5821 ttttttgaac aattgaatgg atgaaagaaa ataaacaggc ccggataaaa ccgtcagtgt
     5881 taagaaaagg ttgaaaagaa aaggcggttt ggaagcatgt tccacatgat ctaaccggat
     5941 tccttcggag atgcccggaa aaaaagaggc gataagcagg tacaggttaa acagcagaaa
     6001 ggggagaaaa tgcagtagcc ttccggtgtt taacctgaag ctatgctgaa taagcgagga
     6061 gatatacagg tataaaaaag gaccgttcag catcagcagc gaaacaaaag aggccgaaag
     6121 caggtggtaa gcggtaaaca acttatgcga gaaaagcccg taaaaacctg tatagaaacc
     6181 caggtaaatc agccaacaaa aaagaatttt gtcgtgcctg gccatctttt tctgaaaaag
     6241 cagcaatgca aaaaaaatag cgttgaatcc cgcgattaaa aacaggtagt gcatctttta
     6301 agctggataa agaatgtaag aaaaccccac aacttgtttt cgaggactaa taatcagaag
     6361 agcaactctt acttaaatac ctggtcaatc cccgagaacc ccataaaggc cattgcgata
     6421 attccggcag taatcagggc aataggaata ccctgcatgg cttttgga
//
LOCUS       DAOSDR010000176         6455 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrimonas sp. isolate AMR_MDS_1826
            NODE_4340_length_6455_cov_4.488906, whole genome shotgun sequence.
ACCESSION   DAOSDR010000176 DAOSDR010000000
VERSION     DAOSDR010000176.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863355
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrimonas sp. (wastewater metagenome)
  ORGANISM  Petrimonas sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Dysgonomonadaceae; Petrimonas.
REFERENCE   1  (bases 1 to 6455)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6455)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:10:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,720
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 35
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6455
                     /organism="Petrimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4340_length_6455_cov_4.488906"
                     /isolate="AMR_MDS_1826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2023866"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..752
                     /locus_tag="PLI30_07915"
     CDS             <1..752
                     /locus_tag="PLI30_07915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019539595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HOI79515.1"
                     /translation="KKVMEFATQIAKEIKQKIHCQTRLTASAGVSYNKFLAKIASDYN
                     KPNGLYVITPEKAEKFLEKLGIEKFFGVGRVTAHRMHELGIRNGFDLKQHSEKELVHH
                     FGKAGALYHQFARGIDEREVESEYVRKSLGAEETFAEDLHELVDLLRALDDIAREVHR
                     RAEKRGFLAKTVTLKIKYADFAVVTRSKTVVNYLRTYDQLFDIGKELLLQVDDIEERK
                     IRLMGLTLKNAGTDQVVLTNQPLQLTLEFKE"
     gene            complement(742..1281)
                     /locus_tag="PLI30_07920"
     CDS             complement(742..1281)
                     /locus_tag="PLI30_07920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HOI79516.1"
                     /translation="MRLSKFILNKILGWKVINTLPDASKCIIVVAPHTSNWDFIIGKL
                     AYSSIGRTANFLIKKEWFIFPFNLFFKSIGGIPVERSKNNSMTGFLAAEFEKHNKLHL
                     AITPEGTRKPVKEWKKGFYFIAVKANVPILLIGLDYSKKEASLLDLFYPSGDYKTDIV
                     KIKSYYKDIKGKHPENFIL"
     gene            complement(1382..3316)
                     /locus_tag="PLI30_07925"
     CDS             complement(1382..3316)
                     /locus_tag="PLI30_07925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005682170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HOI79517.1"
                     /translation="MISIENLTVQFGGFTLLDEISFVLNKDERIALAGKNGAGKSTLL
                     KLMAGAQQPTSGIISYPKEVTVGYLPQQMKLSNERTVKEEAALAFEHLKKLEQELEVL
                     HRQLAERTDYESDEYHHIIQRSTDVQELLLMSGINDFEAETEKTLLGLGFNRSDFNRP
                     TQEFSGGWRMRIELAKILLQSPDVLLLDEPTNHLDIESIQWLETFLISRTNAVMLVSH
                     DKAFLDNVTNRTIEIVLGNIHDYKVNYSKYLELRKERREQQQRAYENQQKQIQDTEAF
                     IERFRYKATKSNQVQSRIRQLEKIERIEVDEVDNSRLNLKFPPAPRSGSYPVVTENLA
                     KKYGDHQVFSNVTLTINRGEKVAFVGKNGEGKSTLVKCIMGEIGFDGNLELGHNVKIG
                     YFAQNQAQLLDENLTVFETIDYVAAGDIRTKIRDILGAFMFGGEASDKKVKVLSGGER
                     TRLAMIRLLLEPVNLLILDEPTNHLDIASKEVLKKAIREFDGTAIIVSHDRDFLNELV
                     SKVYEFGGGGVREHMGGIYDFLSKKKLENLQQLELSKSPATSKQKEKEEHLSENKLSY
                     QEQKEQNRLIKRKEKEVQEAEEKITKMEERLSEMETQLSTPEGAVNFDLLQKYLETKN
                     KLDAVMNKWERITLELENLK"
     gene            complement(3320..4687)
                     /gene="hisS"
                     /locus_tag="PLI30_07930"
     CDS             complement(3320..4687)
                     /gene="hisS"
                     /locus_tag="PLI30_07930"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006841450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HOI79518.1"
                     /translation="MQKPSIPKGTRDFSPEEMAKRNYIFDTIKNVYRLYGFRQIETPA
                     MENLSTLMGKYGEEGDKLLFKILNSGDFLSSISTDDVKEGNSAKTAGKISEKGLRYDL
                     TVPFARYVVMHRNEISFPFKRFQIQPVWRADRPQKGRYREFFQCDADVVGSDSLLNEV
                     ELIQIIDEVFRRLNIRVSVKLNNRKILSGIAEIIGEADKITDITVAIDKLDKIGLANV
                     NEELRGKGISETAIQQLQPIILIGGTNNEKLTTLKSVLSSSETGLKGVEELEFILNKT
                     GLLTIGCGLELDLTLARGLNYYTGAIIEVKALDVEIGSITGGGRYDNLTGIFGLSGVS
                     GVGISFGADRIYDVLDQLSLFPDNSTHTTDVLFVNFGDKEESCLLPILSQLRSNGICA
                     EIYPEAAKMKKQMSYADNNNIPFVALVGENEIKEGKVTLKEMQSGEQTALSVDELIQK
                     LAHQK"
     gene            complement(4775..5755)
                     /locus_tag="PLI30_07935"
     CDS             complement(4775..5755)
                     /locus_tag="PLI30_07935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007574815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HOI79519.1"
                     /translation="MKKFKQLTVERRYQISALLQRGSTQKQIAGIVGVSESTISRELS
                     RNKAKRGKYSAARAQMLADERKERFKRKRSFSSSMQRFIDKKLREEQWSPEQINGYCK
                     VNGIEMVSVERIYQHIRKDKAQGGDLYTFLRHKLKHRRRPVSDTRVHIKDRVGIEHRP
                     AIVDQKKRFGDLEIDTVIGKDGKGAILTIVERTKAFVLMEKLEKGKNAEAVKETVIRL
                     LMPYKGKIHTITSDNGKEFAEHKAIASALDIDFYFANPNSSWERGLNEYTNKLVRQYI
                     PKGTDFNDVNSEKVKEIQYKLNRRPRKKLNYKTPAQLFFASLENKIAFAS"
     gene            complement(5929..>6455)
                     /locus_tag="PLI30_07940"
     CDS             complement(5929..>6455)
                     /locus_tag="PLI30_07940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005788731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HOI79520.1"
                     /translation="AAALKQSKQYNQILLARPIVSLSNKDLGYLPGDEKQKIAPYMQP
                     LFDNLNVIKSQLGQNSAELRSIDEMQKSGKLEIEALAFIRGRSLSETYCIVDEAQNLT
                     PHEVKTIITRAGEGTKMVFTGDLQQIDSPYLDSQSNGLAYMIDKMKGQHVFAHVNLVK
                     GERSELSELASNLL"
BASE COUNT         1575 a         1422 c         1405 g         2053 t
ORIGIN      
        1 ataagaaagt gatggagttt gcgacgcaaa tcgctaaaga aattaagcaa aagattcact
       61 gccagacccg gttaacagcc tctgccggag tttcctacaa taagttttta gctaaaatcg
      121 cttccgatta caacaaacca aacgggttat acgtgattac gcctgaaaaa gctgaaaagt
      181 ttctcgagaa gctgggcatt gaaaagttct ttggtgtagg cagggttacc gcccatcgga
      241 tgcacgaatt aggaataaga aatggattcg acctgaaaca gcattcggaa aaggagcttg
      301 tacaccattt cggtaaggcc ggagccctgt atcatcagtt tgcccgcggg atcgatgagc
      361 gagaagtgga gtcggaatac gtacgaaaat cgttgggtgc agaagagacg tttgcagaag
      421 accttcacga actggttgat ctattacgtg ccctggacga tattgcccgt gaagtacacc
      481 gtagagccga gaaaagaggt tttctggcaa aaacagtgac cctgaaaatt aaatacgctg
      541 actttgccgt tgttacccgt agtaaaaccg ttgtcaatta tttaaggacg tatgatcagc
      601 ttttcgacat aggcaaagaa ttgcttctac aagtagacga tatcgaagag cgaaaaatcc
      661 ggctgatggg gttgactttg aaaaatgccg gtaccgacca ggtagttttg acaaaccaac
      721 cgcttcagtt aacccttgag tttaaagaat gaaattctca ggatgtttcc ctttgatgtc
      781 cttgtaatag gatttgattt tcacaatatc ggttttgtag tcgcccgacg gatagaacag
      841 gtcgagaagt gaggcttctt ttttgctata gtccaatccg atcaatagaa taggaacatt
      901 agccttcacg gcaataaaat aaaatccttt tttccattct ttcaccggct tacgggttcc
      961 ttcgggagta atggctagat gtagtttgtt gtgtttttca aactctgccg ccaaaaaacc
     1021 ggtcattgaa ttatttttgc ttcgttccac cggaatccct ccgatacttt tgaagaagag
     1081 attaaatgga aaaatgaacc attctttttt tatcaaaaaa ttggcggttc tgcctataga
     1141 ggagtaggcg agtttaccaa taataaagtc ccagttgctt gtgtgcggtg caactacaat
     1201 gatgcacttg gatgcatcgg gtagggtgtt tataactttc cagccaagta ttttgtttaa
     1261 tatgaatttg ctaagtctca ttttagttca acgtttttta tttttaattt tttagacgaa
     1321 aaaaataatg tgatagggtt aaattgtcat attgaacgat gacgaaaatc tatcatatat
     1381 tctattttaa gttctctaat tccaacgtta tgcgttccca tttgttcatc acagcatcta
     1441 atttattctt ggtctccagg tacttttgca ataaatcgaa attgactgcg ccttcgggtg
     1501 tggataattg cgtttccatt tccgataaac gttcttccat tttagtgatt ttctcttcgg
     1561 cttcctgcac ttctttttct tttcgcttaa ttaaccggtt ttgttctttc tgttcctgat
     1621 acgaaagctt attttctgac aggtgttctt ctttttcttt ctgcttggat gtagcaggag
     1681 attttgataa ttccagttgt tgaaggttct caagtttctt tttggatagg aaatcataaa
     1741 ttccacccat atgctcccgt actccgccac cgccaaactc atagactttg ctaacgagtt
     1801 cattgagaaa atcccggtcg tgcgacacga tgatggctgt gccgtcgaat tcgcgaatgg
     1861 cctttttcaa tacttctttc gatgcaatat ccaggtggtt ggtcggctca tcgagaataa
     1921 gcaggttcac cggttcgagc aagagtcgga tcatcgccaa ccgggtgcgt tcgccgccgg
     1981 acaatacttt tactttttta tcggatgctt caccgccaaa cataaatgct cccagtatat
     2041 cccggatttt ggttcggata tcacctgcag caacgtagtc gatggtttca aaaaccgtca
     2101 ggttttcatc gagtaactgt gcctggttct gagcaaaata accaattttt acgttatgtc
     2161 cgagctccag attcccatca aacccaatct ctcccataat gcatttcacc agtgttgatt
     2221 ttccctcgcc gttttttccc acaaaagcaa ctttctcacc ccggttgatg gtcagtgtta
     2281 cgtttgagaa tacctgatga tcgccatatt ttttggctaa attttcagta acgaccggat
     2341 aggagcctga tcgtggtgca ggaggaaact ttaaattcag gcgtgaattg tccacttcat
     2401 ccacttcaat gcgttcgatt ttttcgagtt gtctgatgcg tgactgtacc tggttggatt
     2461 tggtggcttt gtatcgaaat cgttcgatga atgcctcggt atcctgaatt tgtttctgct
     2521 gattttcgta agcccgttgc tgttgttcac gcctctcttt tctaagttcg aggtatttgg
     2581 agtaatttac tttgtaatcg tggatattcc ccaacacaat ttcgatagtg cggttggtta
     2641 cgttgtctaa aaaagctttg tcgtgtgaaa ccagcatcac ggcattggtt cgtgagataa
     2701 ggaaggtctc tagccattgg atagattcaa tgtccaggtg gttggtcggc tcatccagca
     2761 ggagtacgtc gggtgattgg agcaggatct tggccagctc aatgcgcatc cgccatccac
     2821 cgctgaattc ttgcgtcggg cggttaaagt cggaacgatt gaatcctaac cccagtaatg
     2881 ttttctcggt ttcggcttca aaatcattga tgcccgacat cagaagcaac tcctgcacgt
     2941 cggtggatcg ttgaataata tggtggtatt cgtccgattc ataatcggtc cgttcagcca
     3001 attgccggtg aagcacttcc agttcctgtt ccagtttttt taagtgctcg aacgcaagtg
     3061 ctgcctcttc ctttaccgtt cgttcattgc tgagcttcat ctgttgtggc aggtagccca
     3121 ccgtgacttc tttcgggtac gaaatgatgc cgcttgtagg ctgttgtgct cccgccataa
     3181 gttttaacag cgtggatttt cccgcgccgt ttttcccggc gagtgcaatt cgttcgtcct
     3241 tgttcagaac aaaagaaatt tcatcgagca gggtaaaccc gccgaactga acggttagat
     3301 tttcgatgga aatcatggat tacttttgat gtgccagttt ttgaataagt tcatcaacag
     3361 acagagccgt ttgttctccc gattgcatct ctttcagcgt aactttcccc tcttttattt
     3421 cgttttctcc caccaatgcc acaaaaggaa tgttattatt gtcggcatac gacatttgct
     3481 ttttcatctt ggcggcttcg ggataaattt cggcacaaat cccgttgctt cgcaattgcg
     3541 agaggatggg gagcaggcat gactcttcct tgtcgccaaa gttaacgaac agcacatcgg
     3601 ttgtgtgcgt ggagttatcg ggaaagagac tgagttgatc aagcacatcg taaatacggt
     3661 cggcaccgaa agagattcca acacccgata cacccgaaag tccgaaaata cccgtaaggt
     3721 tgtcgtaacg gcctccgccc gtgatacttc ctatttccac atccaacgct tttacttcga
     3781 tgatggctcc agtataataa ttgaggccgc gagccaaggt gaggtcaagt tcgaggccgc
     3841 aaccgatagt tagcagcccg gttttattga gaatgaattc cagctcttcc acacctttca
     3901 ggccagtttc ggaagaagac agcacgcttt taagtgttgt cagtttctca ttattggttc
     3961 ctccaatcag aataatgggt tgcagttgtt gtatggcggt ttcggaaatg ccttttcccc
     4021 ggagctcttc attcacgtta gccagcccga ttttatccaa cttatcgatg gcaacggtaa
     4081 tgtcggtgat cttatcggcc tcgccgataa tttcggcaat ccctgaaagg attttccggt
     4141 tattgagttt taccgataca cggatattga gtctgcggaa tacttcgtca ataatttgta
     4201 tcagctccac ctcgttaagc aatgagtcgg aacctaccac gtcagcatcg cattgaaaaa
     4261 attcgcgata acgtcctttt tgcggccggt ctgcgcgcca gacgggttga atttgaaacc
     4321 gcttgaaagg aaaagatatc tcattgcggt gcattactac gtatcgggca aagggtacgg
     4381 taaggtcgta tcgcaatcct ttttctgaaa tcttaccggc cgtttttgcc gagttccctt
     4441 cctttacatc atcagtactt atactcgaaa gaaagtcacc cgagttaagg attttgaaca
     4501 ggagcttatc gccctcttca ccgtatttcc ccatcagcgt ggaaagattt tccatagcag
     4561 gtgtttcaat ctgccggaat ccgtagaggc gatatacgtt tttgattgta tcaaaaatat
     4621 agttgcgttt cgccatttct tcgggcgaaa aatcacgcgt acctttaggt atggagggtt
     4681 tttgcatcgt tgattttctt tgtttggaac tgcaaagata atgaattttg agttaccgtg
     4741 gtatctgttt cggccataac aaaagcgcgt agattcaact cgcaaatgca attttatttt
     4801 ccaaagatgc aaaaaataat tgagccggtg ttttatagtt caattttttt cttggtcttc
     4861 ggtttaattt gtattgtatc tccttcactt tttcactatt aacatcatta aagtcagttc
     4921 ctttcgggat gtattggcgt acgagtttat tggtgtattc gttcaatcct ctttcccagg
     4981 aactgtttgg attggcaaag taaaaatcaa tatccaaagc gcttgcaatg gctttgtgtt
     5041 ctgcaaactc cttgccgtta tcggatgtta tggtgtgaat ctttccttta taaggcatta
     5101 acagacggat gacagtctcc tttactgcct cggcattctt ccccttttcc aatttttcca
     5161 ttagcacaaa cgccttggtt cgctccacga tggttaagat ggcacccttg ccgtctttgc
     5221 cgattacggt atcgatttcc aaatctccaa atcgtttttt ctggtctacg attgccggtc
     5281 ggtgttcaat ccccaccctg tctttaatgt gtacccgggt atccgatacc gggcgtcttc
     5341 tgtgcttgag cttgtggcgt aagaaagtgt ataaatctcc tccttgcgcc ttgtctttgc
     5401 gaatgtgctg ataaatcctt tccacgctta ccatctcaat gccgttgact ttgcaatagc
     5461 cgttaatctg ttcgggagac cactgttctt cccgtagttt tttgtcaatg aaccgctgca
     5521 tggatgaact gaagctacgc ttgcgcttga agcgttcctt gcgttcatcg gcaagcattt
     5581 gagcgcgagc cgcagaatac ttcccacgct tggctttatt gcgggacaac tcccgactta
     5641 tggttgattc cgatactcct acaatccctg caatctgttt ttgtgtactt cccctttgaa
     5701 gtagagctga tatttggtat cttcgctcta cggtcaattg tttaaatttt ttcataatac
     5761 aactattata aacattttgg ccaactttcc caagggaacg cgttcccttg ggaatctttt
     5821 tgaccattaa taaatgatat ttagttgcat ttactaattg aatttacggc gcattgcagt
     5881 aatttgcaac acgcttgaat gagttcaaca aagtctttgt tcttgaatct ataaaagatt
     5941 gcttgccaat tccgataatt cgctcctttc gccttttacc agatttacgt gagcaaaaac
     6001 atgctgccct ttcatcttgt cgatcatgta tgccaggccg ttggattgag agtcgaggta
     6061 aggtgagtcg atctgttgca ggtcgcccgt aaaaaccatt ttagtacctt cccctgcacg
     6121 ggtaatgatg gttttcactt cgtgtggcgt taggttctgg gcttcatcaa caatgcaata
     6181 cgtttcgctt aagctgcgcc cgcggataaa tgctagggcc tcgatctcaa gtttaccgga
     6241 tttctgcatc tcatcaatgg agcgaagttc agcactgttt tgtccgagct gtgattttat
     6301 tacattcagg ttgtcgaaca gcggctgcat ataaggggcg attttttgtt tttcgtcgcc
     6361 cggcaaatat cccaaatcct tgttggacaa ggaaacgatg ggcctagcga gcaaaatctg
     6421 gttgtattgc ttactttgtt tcaaagctgc tgcta
//
LOCUS       DAOSDR010000177         6444 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrimonas sp. isolate AMR_MDS_1826
            NODE_4348_length_6444_cov_5.442323, whole genome shotgun sequence.
ACCESSION   DAOSDR010000177 DAOSDR010000000
VERSION     DAOSDR010000177.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863355
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrimonas sp. (wastewater metagenome)
  ORGANISM  Petrimonas sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Dysgonomonadaceae; Petrimonas.
REFERENCE   1  (bases 1 to 6444)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6444)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:10:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,720
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 35
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6444
                     /organism="Petrimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4348_length_6444_cov_5.442323"
                     /isolate="AMR_MDS_1826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2023866"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2067)
                     /locus_tag="PLI30_07945"
     CDS             complement(<1..2067)
                     /locus_tag="PLI30_07945"
                     /inference="COORDINATES: protein motif:HMM:NF025851.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 95 protein"
                     /protein_id="HOI79521.1"
                     /translation="MKILSILLAAAALLSCTPPAETAQELALRFNTPAAAWEETLPLG
                     NGRIGMMPDGGIDKELIVLNDITMWSGSEDPEALNPEALNYLPKIRELLLTGKNGEAQ
                     RMMYDHFQCGGLGSSGGKSKDAPYGCFQMLGDLHINYSYPQTEDTGNYVRTLSLNDAV
                     ASTVFMKGETTFTREYISSHADDVLAVHVAADKKGSVSFEVSLSRPERATLSVQENTL
                     IMEGQLNDGYGTDKGVRYLTKVQIVNKGGELTAGSNTLAIANADEAVILISTSTDMLD
                     KEYTSTVDSLLEQAKKRSFEKMKQAHIAAYKEKFDRVELWLGEQDNTTPTNERLAGFQ
                     TDDDPAFAALYFQYGRYLMISGTDENSLPLNLQGLWANQVQTPWNGDYHLNINVQMNY
                     WPVEVCNLSELHKPLIDFTQSIVPSGEATAQTFYGANGWLAHMMSNPWKFTAPGEHAS
                     WGATNTGGAWLCEHLWEHYAFTQDKEYLRTVYPTLAGAAQFFLNSMISEPRNGWLVTA
                     PSSSPENAFYMPGSDDRIFVCMGPTMDVQIVNELFTNVLSAAAILGIEDETTTNIRET
                     LPKLPPMQISPEGYLQEWLEDYKEVDPKHRHVSHLYGLYPSNQISPNTTPELAAAARE
                     TLERRGDAGTGWSRAWKINFWARLYDGNRAFKLLKSLLEPTSGSEVNMHRGSGTYANL
                     FCAHPPF"
     gene            complement(2092..3930)
                     /locus_tag="PLI30_07950"
     CDS             complement(2092..3930)
                     /locus_tag="PLI30_07950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014224675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HOI79522.1"
                     /translation="MLKNIYLLFISLIICTGCSTKQPEYTFGVKPDTKEDASGAAVKL
                     IGQLQARKDTVHITVKIPKGRYDFYPDSAFTREYYISNHDQDNPKKVGFALENLQNVT
                     IDGQGSEFVFHGRMIPFAILKGQNITLKNFSVDFELPALRQLNILEVDPGKDELLAKI
                     YPGGNYRIDTEKLVLLGEGYEVTPQGSMAFRPDKRLTYIRRDVNFNPLSVTEASPDVL
                     RIKGWEQIKLTSPGERYVLRSWKRPTPGIFISECTNTVLENVKVHYAEGMGLLAQMSE
                     NITLDRFSVCLKGEDDPRFFTTQADATHFSACKGVIVSKNGLYEGMADDAINVHGTYL
                     RVTKRLNDTTLQARYMHPQAWGFKWGETGDSVQFVESEKMERVGSHFNTITSIKAVDK
                     PTEFGAKEFEITFAATLPQEISETGKFGIENLTWTPEVVFSDNIIRNNRARGALFSTP
                     KRVICENNLFDHTHGTAILLCGDCNGWYETGACKEVIIRNNRFINALTATYQFTNAVI
                     SIYPEIPNLKDQQQFFHSGIVIENNTFETFDRPLVYAKSTDGLIFRNNTVTYNTEFEP
                     FHWNKHPFFFERVSNVLIENNRFENGWDAEKDIRTEHSAEDAITVK"
     gene            complement(3933..4514)
                     /locus_tag="PLI30_07955"
     CDS             complement(3933..4514)
                     /locus_tag="PLI30_07955"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HOI79523.1"
                     /translation="MRNHIFNSLLTVATITYSGNLAAQQKPNVIFIYADDLGKGLLSA
                     YGQKQYTTPNIDKLVKQGISFNRAYGCMLSGDIFNGNASMAGLKRSNLQGGVNVPRIF
                     YWKGTFAENILLPDNRYVVYSSNLGAAIVENTGWKLRHYAKKNIFELYNLNTDPQERN
                     NLIDNYPQIAEKLINILIRECESDLRNGINKTG"
     gene            complement(4528..4746)
                     /locus_tag="PLI30_07960"
     CDS             complement(4528..4746)
                     /locus_tag="PLI30_07960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI79524.1"
                     /translation="MKIENIVVSGTAKDIHQQLWNTSYPLYKYQNKEKAKEILKQFPN
                     VEYIMDGIYASPDEEYLDVKNIHAILAD"
     gene            4900..6342
                     /locus_tag="PLI30_07965"
     CDS             4900..6342
                     /locus_tag="PLI30_07965"
                     /inference="COORDINATES: protein motif:HMM:NF013752.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HOI79525.1"
                     /translation="MLQDKDIKKIQELKADFTPGRVSAEDIFSRFKALKLSEGLSEFK
                     WFKTRGYDFKMVLALLVSMVVSSDKTVNSYLNSPTGEGSTMGKDVFYRLKNSSIICWR
                     MLMWHLVHRFLTVTSKDSDVDDTSSRYLIFDDTTLSKTGKRIEKIGKVWDHVTNSYVL
                     GFKLLVMMYWDGKSSIPLDFSLHREKGKKQERPFGMTKKQIRKQYSCKRIKDSYTAKR
                     VEELDTNKIQMVLRMFFTAIYRGLRVDYVLVDSWFTCDALIQAIRSVKDKEVHLIGMY
                     KFAKTKFEYQGKSLTHAQINNMLGKPRRCRSLGYQYKQARVLYQGVEICLFFSRRGKN
                     DKWKVLLTTDTKLTFKGLIGHYQVRWVVEVFNRESKQLLNLGRCQSSNFDAQVAETTI
                     SMIAYLLLTLRFRYDNYESKGALYRSMNADVLRETLDRRLWGLFIELVCSVCEVLEMD
                     ADDLLEKMLANPKAESMIIALCCSVMVKDD"
BASE COUNT         1557 a         1429 c         1639 g         1819 t
ORIGIN      
        1 gaacggcgga tgcgcgcaga ataggttagc gtacgttcca gacccgcggt gcatgtttac
       61 ttcacttccg gaagtgggct ccagcaggct tttaagtagc ttgaaagcac ggttcccgtc
      121 gtacaagcgt gcccagaaat tgattttcca tgcacgggac catccggttc cggcatcacc
      181 acggcgttcc aacgtttcgc gagctgctgc ggccagttcg ggggtagtgt taggcgatat
      241 ctggttcgac ggatacagcc cgtagaggtg tgagacatgg cggtgcttgg gatcaacctc
      301 cttatagtcc tcgagccact cttgcaaata accttcgggg ctgatctgca tgggaggaag
      361 tttcggcagt gtttcgcgga tattggtggt tgtctcgtct tcgataccga gaattgctgc
      421 cgccgacagt acgttggtaa acagttcgtt cacgatctga acgtccatgg tcgggcccat
      481 gcatacaaaa atgcgatcgt cgctcccggg catgtaaaat gcattctctg gcgatgatga
      541 cggggcggtg acgagccatc cgttcctggg ttcggaaatc atgctgttga ggaagaactg
      601 cgctgctccg gcgagcgtcg ggtaaacagt gcgcaggtat tccttatcct gggtaaaggc
      661 ataatgctcc caaaggtgtt cacaaagcca cgctcctccc gtgttggtcg ctccccacga
      721 agcatgttcg ccgggagccg tgaacttcca cgggttgctc atcatgtgag ccagccagcc
      781 gttggccccg taaaacgttt gggcggtagc ttctcccgaa ggtacaatcg attgggtgaa
      841 atcgatcagg ggtttatgca gttcagaaag attacatact tctaccggcc agtagttcat
      901 ctgtacattg atattcaggt ggtaatcgcc gttccagggc gtctgcacct ggttggccca
      961 caagccctga aggttgaggg gaagcgagtt ctcatcggtt ccgctgatca tcaggtaacg
     1021 gccatactga aaatagagtg cggcaaatgc gggatcatcg tctgtctgga aaccggcaag
     1081 gcgctcgttg gtaggtgtgg tgttgtcttg ttcgcctaac cagagctcta cacggtcgaa
     1141 cttttctttg taagctgcta tatgcgcctg tttcattttt tcgaacgaac gtttttttgc
     1201 ttgttcgagc aagctgtcta ccgtcgacgt gtactctttg tcgagcatgt cggtggaggt
     1261 ggaaatgagg atgacggcct catcggcatt ggcgatagcc agggtattgg agcccgcggt
     1321 cagttccccg cctttgttca cgatttgtac cttggtaagg tagcggacac ccttatcggt
     1381 accgtatcca tcgttgagct gcccttccat gataagggta ttctcttgca ccgaaagggt
     1441 tgcccgttcg ggacggctca ggcttacttc aaatgagact gaccctttct tatcggcagc
     1501 cacgtggaca gccagcacat cgtcggcgtg agaggagata tactcgcgcg taaaggtggt
     1561 ttcacctttc ataaacacgg tggatgccac tgcgtcattg agcgaaagtg tcctcacgta
     1621 attacccgta tcttccgttt gtgggtagga gtagtttatg tgcaggtccc ccagcatttg
     1681 aaaacagccg tagggcgcat ctttgctttt tcctccggaa gaaccgagcc cgccgcactg
     1741 gaaatgatcg tacatcatgc gttgtgcttc cccgttcttt cctgtaagca gtaactcccg
     1801 gattttcggc agataattga gtgcttcggg gttcagcgct tcagggtctt cgctgcccga
     1861 ccacatagta atgtcgttga gcacgataag ttctttgtca ataccgccat ccggcatcat
     1921 accgatacgt ccgttgccga gaggcagggt ctcttcccac gccgcggcag gcgtgttaaa
     1981 ccgcagggcg agttcttggg ccgtttcagc aggtggcgtg caggaaagca atgccgcagc
     2041 tgcaaggaga atggataaaa ttttcatcga attgttcatt taagtggttg attattttac
     2101 ggtgatggca tcttctgccg agtgttctgt tcgtatatct ttctccgcat cccatccgtt
     2161 ttcgaagcgg ttgttttcga taagaacgtt gctgacccgt tcaaagaaga acggatgttt
     2221 gttccagtgg aacggttcaa actcggtatt gtatgtaacg gtgttgtttc ggaaaatcag
     2281 tccgtctgtc gatttggcat aaacgagcgg acggtcgaac gtttcgaacg tattgttttc
     2341 aataacaata cccgagtgga aaaactgctg ttgatccttg aggttgggta tttcgggata
     2401 gatggaaatg acggcgtttg taaactggta ggttgccgta agggcgttga taaaccggtt
     2461 gtttctaatg atcacctcct tgcaggctcc ggtttcgtac cagccgttgc agtctccgca
     2521 aagcaagatg gccgtcccgt gggtatggtc gaaaaggttg ttttcgcaga ttacccgttt
     2581 gggtgtgctg aagagtgcac cgcgtgcacg attgttgcgg atgatgttgt cggaaaacac
     2641 tacttcggga gtccacgtca ggttttcaat cccgaatttt ccggtttcgg aaatttcctg
     2701 cggaagtgtg gcagcaaagg ttatttcaaa ctcttttgct ccaaattcgg taggtttatc
     2761 gacagctttg atcgatgtaa tcgtgttgaa gtgactgcct acccgttcca tcttttccga
     2821 ttccacaaac tgtacgctgt ctcccgtttc accccacttg aacccccatg cttgtggatg
     2881 catatatctg gcctgtagtg tggtgtcgtt taatcgtttg gtgactctca ggtaagtccc
     2941 gtgtacattt atggcatcat cggccattcc ttcatagagc ccgtttttcg aaacaatcac
     3001 ccccttacac gccgaaaagt gggttgcatc ggcctgtgtg gtgaaaaacc gggggtcatc
     3061 ttcccctttc aggcaaacgg agaaccggtc taaggtaata ttttcactca tctgtgccag
     3121 caggcccatc ccttcggcat aatgtacctt aacattctcc agtacggtgt tggtgcattc
     3181 gctgatgaag atgccgggtg tgggacgttt ccacgaacgc aggacatagc gttcacccgg
     3241 cgatgttaac tttatctgct cccatccctt gatgcgtaaa acatccggcg aagcttctgt
     3301 aaccgacagc gggttaaaat ttacgtcacg acgtatgtag gtcaggcgtt tgtcgggccg
     3361 gaatgccatt gagccctgtg gggttacttc gtagccttct ccgagaagga caagcttttc
     3421 ggtatcgatg cggtaatttc cgccgggata aattttggcc agcagttcat ctttgccggg
     3481 atccacttcg agtatgttga gctggcgcaa ggcaggaagt tcaaagtcga cggagaaatt
     3541 tttcaaggta atgttttgcc ctttcaggat ggcaaaaggg atcatgcgtc cgtgaaaaac
     3601 aaactccgaa ccttgtccgt cgatggttac attctgcagg ttttcgagtg cgaaccccac
     3661 ctttttggga ttatcctggt cgtggttcga gatatagtat tcgcgtgtga aagcactgtc
     3721 tggataaaag tcgtaccgcc ctttcggaat tttaacggta atgtgcacgg tatctttccg
     3781 cgcttgcagt tgtccgatca gtttaactgc cgcacccgac gcgtcttctt tcgtatcggg
     3841 tttcacaccg aatgtatact ccggttgttt tgtagagcat cctgtgcaga ttatcaagga
     3901 gataaagagt aaatagatgt tttttagcat aattatcctg ttttattaat tccgttcctt
     3961 aaatcactct cgcattctcg aataagaatg tttattagtt tttcagcaat ctgaggatag
     4021 ttgtctatta agttgtttcg ttcctgaggg tcagtattca aattatacag ttcgaaaata
     4081 ttttttttgg cgtaatgccg aagtttccag cccgtatttt caacgattgc cgctcccaga
     4141 ttggaactgt aaaccacata tcggttgtcc ggcaaaagta tgttttcggc gaatgtgcct
     4201 ttccaataaa atatgcgcgg aacgttgaca ccgccttgca ggttacttct tttcaacccc
     4261 gccatggatg cgtttccatt gaaaatatca cccgaaagca tacagccata tgcccggtta
     4321 aaagaaatac cttgctttac taatttatct atattagggg tagtgtattg tttttgccca
     4381 tatgctgaaa ggagcccttt tcccaaatca tcggcataaa tgaagatgac gttgggtttt
     4441 tgttgcgcag ctaaattacc actataggta attgtcgcaa cggtaagtaa tgagttaaaa
     4501 atatgatttc tcattaaaaa tagggtatta atcagctaaa atagcatgaa tatttttaac
     4561 atcaaggtat tcttcatccg gagacgcata aataccatcc attatatatt ctacgttagg
     4621 gaactgtttt aaaatttcct tcgctttctc tttattttga tacttatata gaggataact
     4681 ggtattccat agctgttggt gaatatcttt tgctgtaccc gatacgacta tattttctat
     4741 cttcatttgc tttaatagcg taatgttttt tcttactgat gtttcgcagt taaaaatgaa
     4801 gttgataatc agaggataag agataaaaaa agcagcatat tttagctaat atatcattga
     4861 aaatgattat cttagcgatg gaaaccaaac cattaaaata tgctgcagga caaagatatt
     4921 aaaaagattc aagagttgaa agcggacttt acccccggca gggtttccgc tgaagatatt
     4981 tttagccgct tcaaggcgtt gaagctaagc gaaggtcttt ctgagttcaa atggttcaag
     5041 acccggggat atgattttaa aatggtcctg gccctgctgg tctccatggt ggtcagttca
     5101 gacaagacgg tcaactccta tctgaacagc cccacaggtg aaggttccac catgggcaag
     5161 gatgtgttct atcgcttgaa gaacagttcc atcatatgct ggcggatgct tatgtggcat
     5221 cttgtgcatc gttttctgac ggtgacgtcc aaagatagtg atgtagatga tacctcatcc
     5281 cgttacctga tcttcgatga caccaccctg tctaaaaccg ggaagcgtat agagaagatc
     5341 gggaaggtat gggatcatgt aaccaacagc tacgtgcttg gattcaagct gttggtgatg
     5401 atgtactggg acggtaagtc ctccatacca ctggatttca gtcttcaccg ggaaaaagga
     5461 aaaaagcagg aacgcccgtt tgggatgacc aaaaagcaga tccgcaagca atacagttgc
     5521 aagcgtatca aggactcgta caccgctaaa cgtgtggaag aactggacac gaacaagatt
     5581 cagatggtcc tgcggatgtt tttcacggcc atctatcgcg ggcttcgtgt tgattacgtc
     5641 ctggtggaca gctggtttac ttgcgatgcg ttgatacagg ctatccggag tgtgaaggac
     5701 aaggaagttc acctgatcgg gatgtacaag tttgcaaaga ctaagtttga gtaccagggt
     5761 aagagcctga ctcacgccca gatcaataac atgctgggca aacccaggcg ctgccgttcc
     5821 ctgggttacc agtacaaaca ggccagggtg ctgtaccaag gtgttgagat ctgccttttc
     5881 tttagtcgtc ggggcaaaaa tgacaaatgg aaggttttgt tgacgacgga cacgaaactg
     5941 acgttcaagg gattgatcgg gcattaccag gtaagatggg ttgtggaggt gttcaatcga
     6001 gaatcgaagc aattgttgaa cctgggaaga tgtcaatcca gtaactttga cgcccaggta
     6061 gcagaaacga ccatcagcat gatagcctat ctgttattga cactgcgttt ccggtacgat
     6121 aactacgagt cgaaaggggc actttatcgg tcaatgaacg ccgatgtgct cagggaaaca
     6181 ctcgacagac ggctatgggg gctgttcatc gagttagtct gctcagtatg cgaggtattg
     6241 gagatggatg ccgatgattt acttgaaaaa atgttggcca atccaaaagc agaatccatg
     6301 atcattgcac tgtgttgttc ggtgatggtt aaggacgatt gagaaattgt aaaatataaa
     6361 atgttatagt acagagatat agataataat gcatttccct tctatttctt tgtcaatggt
     6421 ttgagggaag tgcgaaacat gagt
//
LOCUS       DAOSDR010000178         6425 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrimonas sp. isolate AMR_MDS_1826
            NODE_4355_length_6425_cov_4.440502, whole genome shotgun sequence.
ACCESSION   DAOSDR010000178 DAOSDR010000000
VERSION     DAOSDR010000178.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863355
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrimonas sp. (wastewater metagenome)
  ORGANISM  Petrimonas sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Dysgonomonadaceae; Petrimonas.
REFERENCE   1  (bases 1 to 6425)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6425)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:10:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,720
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 35
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6425
                     /organism="Petrimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4355_length_6425_cov_4.440502"
                     /isolate="AMR_MDS_1826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2023866"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..244
                     /locus_tag="PLI30_07970"
     CDS             <1..244
                     /locus_tag="PLI30_07970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI79526.1"
                     /translation="KPAMMHETLVNAVVENKVGSAEIPVGKQSIYNCRNGERIVHDPE
                     RVHICLKLVFQQFGTHVLRKTRAEQHYPCGMINPES"
     gene            complement(564..1763)
                     /locus_tag="PLI30_07975"
     CDS             complement(564..1763)
                     /locus_tag="PLI30_07975"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006743409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HOI79527.1"
                     /translation="MGANKTNQIILRPRKEESLKRFHPWVFSGAIAKTGQGIQEGDIV
                     RVFSASGEFLGVGHYQVGSISVRILSFTDETINEDFYARRISSAYRMRMKIGLLHAGN
                     NTFRLIHGEGDHLPGLIIDMYGKTAVVQAHSVGMDGDKEKIVDALQRVLGKDNLENIF
                     YKSEGTLPFKAGIEADNDYLLGGQNVDPVALENGLRFNIDWLRGQKTGFFIDQRENRR
                     LLEKYAAGKDVLNMFCYTGGFSVYALRGGARSVHSVDSSAKAVSLTDENIRLNFGEEP
                     RHTSFSEDAFKFLKASTANAYDLIVLDPPAFAKHRGAISNALQGYKRLNLAALEKIKQ
                     EGILFTFSCSQAINKEQFRLAVFSAAAISGRKVSILHQLTQPADHPINIYHPEGEYLK
                     GLVLFVE"
     gene            complement(1750..2328)
                     /locus_tag="PLI30_07980"
     CDS             complement(1750..2328)
                     /locus_tag="PLI30_07980"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019538510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HOI79528.1"
                     /translation="MGLTISKEAVAELPSEVFAGKTIVVDHESEVEKAVADLMKHSRL
                     GFDTETKPAFKRGQTHKVALMQLSTMEVCYLFRLNKIGYPDELDEIICNPDIKKIGLS
                     LRDDFAAIRKRSDKKPQNFIDLQSFVDEYDIDDNGLQRIYAILFGKKISKNQRLSNWE
                     ASVLSEAQKNYAAMDAWACLRIYNHLISNGSE"
     gene            complement(2319..2894)
                     /locus_tag="PLI30_07985"
     CDS             complement(2319..2894)
                     /locus_tag="PLI30_07985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019538511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5063 domain-containing protein"
                     /protein_id="HOI79529.1"
                     /translation="MLNKESDNIVYSSTVIDFVTVAVEFCAFLEKEEIASREKWIDKM
                     LKLLPLMYIKASLLPQTVEINDESPETFVKEEDYTRVSATVSSIMGEEDVYLDVFVED
                     MKYSERPVSAFVSENIADIYQDVRNFVSVYQYGLTDQMNDALLICQQNFQAYWGQKLI
                     NVLRPLHSVLYHSEEKDNLLETDLNEEDLWD"
     gene            3030..4202
                     /locus_tag="PLI30_07990"
     CDS             3030..4202
                     /locus_tag="PLI30_07990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005866537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate/amino acid:cation symporter"
                     /protein_id="HOI79530.1"
                     /translation="MKLSKIPLLGRVVIAIVSGIILGQFVPVWFARIFATFNDLFGNF
                     LSFIIPLIILGLVAPAIGELGKGAGRLLLITTVIAYMSTLFSGFFTFFSCQAVFPNII
                     TGAIDTGSVQGIDEGAVKTFFSIGMPPIMDVMSALILSFCIGIGLSLIKGDTLQKAFS
                     DFRDIVTMIIRTVIIPLLPIYIFGMFLSISITGQVYSVIMLFLKVISVIFVLHILLLV
                     MQYVVAGAISRRHPLKALKTMLPAYMTALGTASSAATIPVTLQCALNNKINPSIASFV
                     IPLCATIHLAGSAMKIVGFSLAIMYFFGIPVDPGTFIGFIFMVGITMIAAPGVPGGAI
                     MAAIGIIQSMLGFNEQMIGLMITVYIAVDSFGTACNVTGDGAIALIMDKWAGTSRT"
     gene            complement(4199..4570)
                     /locus_tag="PLI30_07995"
     CDS             complement(4199..4570)
                     /locus_tag="PLI30_07995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HOI79531.1"
                     /translation="MEFIDRLKLFIKSKGLGQTKFEERVGFSRGYISKVKTSIGADKL
                     SNIVEAFPDLNLDWLITGKGKMTSTPVQPAAVEQTTDTLEENSVKYKNKYLEMLEENR
                     VLRLEIEKLRKGTGTRADNPF"
     gene            4735..4956
                     /locus_tag="PLI30_08000"
     CDS             4735..4956
                     /locus_tag="PLI30_08000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI79532.1"
                     /translation="MNNQANTIKPVTKISIPDTLLSLKVGETVMISARTINSSSVRAA
                     ASRLKKIKKAQFIVTEQGLINEIKVTRLK"
     gene            5380..>6425
                     /locus_tag="PLI30_08005"
     CDS             5380..>6425
                     /locus_tag="PLI30_08005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008771785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HOI79533.1"
                     /translation="MLVFWWICSVGLYAQLRVQGTVTDLDGTPMIGVNVIEKGTLNGS
                     VTDLNGNYTLTVASAESVLHFSYVGYVAEDVTVGDNRQINLVMREEAESLDEIVVVGY
                     GTQVRKDITGSVAVVNTEQLLASTGSSAAQQLQGKTPGVYIGQTGSPGSATMVRIRGV
                     NTINDNGPLYVIDGVSTRNQNLSSLNPNDIESMQVLKDASSAAIYGAQAANGVILITT
                     KQGTGSGQPKISYDAYFGLQKTGKRYDLLNAADRLAVEWESQQNSFSLRGVEGIPEHA
                     QFGKAVNPVIPNYLTTTGAGGRQDISLDDYSFPNNQIVPYSDTDWWDAIDRVAPMQNH
                     QLTLTGGGAKGRYL"
BASE COUNT         1667 a         1380 c         1449 g         1929 t
ORIGIN      
        1 aaaaccggcc atgatgcatg aaactttggt aaacgccgtt gtggaaaata aggtcggttc
       61 cgctgaaatt ccggttggaa agcaatcgat ttacaactgc cgcaacggag aacggattgt
      121 acacgatccc gaaagggttc acatttgcct gaaacttgta ttccagcagt ttggaaccca
      181 tgttctccga aaaacaagag ccgaacagca ttatccctgt ggaatgatca atccggaaag
      241 ttgattccgg aataattacc ggtgtcctga aatccatcgc taactaaatt cttgtacaaa
      301 aatagcgatt aaccttccaa cttcgaaatc tgaatgatta taatttgtga aaaggggaaa
      361 tgaaatggct ataaaaaagg aaaggttgtc tcacgacaac caatcttcat tgttaacctt
      421 aaatctaata ccatgaaaaa cacagtgcaa atataaaggc tttttttaca ctaaaaaaat
      481 atttcagggg aaaaatcgct ctattttgca tcaattaacc taaaaatgct gttttgaatg
      541 aataataagt gttttttcac actttattct acgaataaaa ccaatccttt caggtattca
      601 ccctcggggt gataaatgtt aatgggatga tcggccggtt gggtaagttg gtgcagaatg
      661 ctgaccttcc ttcccgaaat agcggctgca ctgaaaactg ccaggcggaa ctgttctttg
      721 ttaatggcct gggaacagga gaaggtgaac aggattcctt cctgttttat tttctccaat
      781 gctgccaggt tgagtctttt gtacccttgc agggcattgc tgatggctcc tcggtgcttg
      841 gcaaatgccg gcggatcaag aacaatcagg tcgtaagcat tcgctgtaga tgccttcagg
      901 aatttaaacg catcttccga gaaagaggtg tgtcggggtt cttctccgaa gttgagcctt
      961 atattctcgt cggtaagtga tacggctttg gcagaactgt cgacggaatg aaccgaccgg
     1021 gcccctccgc gcagcgcgta tacagagaat cctccagtat agcaaaacat gttcagtacg
     1081 tctttaccgg cagcatactt ttcgagcaac cggcgatttt cacgttgatc aataaagaag
     1141 ccggttttct gcccgcgcag ccaatcgata ttgaatctca atccgttttc cagggcgacg
     1201 gggtctacgt tttgcccgcc cagcaggtaa tcgttgtcgg cttcaatgcc tgctttaaag
     1261 ggaagtgtcc cttctgattt ataaaaaata ttttcaaggt tatcttttcc cagtacacgc
     1321 tgtaatgcgt caacgatttt ttctttatcg ccatccattc ctacagaatg tgcctggact
     1381 accgccgttt ttccgtacat gtcgataata agtcccggta aatggtctcc ttcgccatga
     1441 atcaaccgga aggtgttgtt gccagcgtgc aacaatccaa tcttcatgcg catccggtat
     1501 gcggaagaaa tgcgacgcgc gtagaaatcc tcatttatgg tttcgtctgt aaaggacaat
     1561 atgcggacgg aaatgctgcc gacctggtag tgccctacac ccaggaattc tccactggca
     1621 gaaaaaaccc gtacaatgtc tccttcctga atgccttgcc cagttttggc gatggcgccg
     1681 gaaaagaccc agggatgaaa ccgcttgagt gattcttctt ttctggggcg caggatgatc
     1741 tgatttgttt tattcgctcc cattgctgat caggtggttg taaatacgta ggcaggccca
     1801 tgcatccata gctgcgtaat ttttttgagc ttcgctcagg acgctggctt cccaattgga
     1861 gaggcgttga tttttagaaa tctttttccc aaaaaggata gcatagatcc gttgcaaacc
     1921 gttgtcgtca atgtcgtatt catcaacaaa agactgcagg tcgataaagt tttgcggttt
     1981 tttgtcggag cgttttcgga tggcggcaaa atcgtcacga agtgaaagtc ctattttctt
     2041 gatgtccggg ttgcagatga tttcgtccag ttcatcggga tatccgattt tgtttaaccg
     2101 gaagagataa catacctcca tggtggaaag ctgcatcagc gccactttgt gagtctgtcc
     2161 ccgtttaaat gccggtttgg tttccgtgtc aaaccctaat cgggaatgtt tcatcaggtc
     2221 tgccaccgct ttctctactt cactttcgtg atctacaact attgtctttc cagcaaaaac
     2281 ctctgacgga agttctgcta ctgcttcttt tgaaatggtt aatcccacaa atcctcctcg
     2341 tttaaatcgg tttccagtag attgtctttc tcttcgctgt ggtaaagtac ggagtgtaac
     2401 ggtctcaata cattgatcag tttttgtccc cagtatgcct gaaagttttg ttggcaaatg
     2461 agaagtgcgt cattcatctg atcggtcaag ccatattgat aaaccgaaac aaaattcctt
     2521 acatcctgat agatgtcggc gatattttcc gaaacaaatg ctgaaaccgg acgttcgctg
     2581 tatttcatat cctcaacaaa aacatccaga taaacatcct cctcgcccat gatggatgaa
     2641 acagtggccg atactcgggt ataatcctct tctttgacaa acgtttcggg agattcatcg
     2701 tttatttcca cagtttgagg caggagcgac gccttgatgt acatcagggg caggagcttg
     2761 agcatcttgt cgatccactt ttcccgcgag gcaatttctt ccttttccag aaaggcgcaa
     2821 aattctacgg cgaccgtcac gaaatcgatt acggtagatg agtaaactat attgtcggat
     2881 tctttattca acattttatt accaattgta ctgcaaaagt actcatttat tttcagacaa
     2941 cgatgagggt aatgctcaat tcaatagaga ttaagcgtgg tttatccgga aaaagactta
     3001 cttttgcgct aaatttttta tccggaaaaa tgaaactatc taaaatacca ttgttgggac
     3061 gcgttgttat cgccattgtc tcgggaatta tcctcggtca gtttgtgccg gtgtggtttg
     3121 cacgaatctt tgccacgttt aacgatttat tcggtaattt cttgtcgttt atcattccgc
     3181 tgattattct cggcttagtt gctcccgcta ttggcgagtt agggaaagga gcggggagac
     3241 tcttgctgat cacaaccgtt attgcgtaca tgtctactct tttttcgggt ttttttacct
     3301 ttttttcgtg tcaggctgtt tttccgaata taataaccgg cgcaatcgac accggatcgg
     3361 tacagggcat tgatgaaggt gctgtgaaaa cgtttttcag tatagggatg cctcctatta
     3421 tggatgtgat gtcagctttg attttgtcgt tttgcatagg gatcggactc tcccttatca
     3481 agggtgatac gttgcagaaa gctttttctg atttccgcga tattgtgacg atgattatcc
     3541 gtacggtaat cattccgtta ctacccattt atatttttgg aatgtttctc tccatctcca
     3601 tcaccggaca ggtttactcc gtgattatgc tttttctgaa agtgatttcg gtgattttcg
     3661 tgttacatat cctgctgctg gttatgcagt atgtggtagc aggcgccatt tcgcgtcgtc
     3721 atccgctgaa agcgttaaag acgatgctgc ctgcttatat gacggcattg ggaacggctt
     3781 cttcagcggc aaccatccct gtgacgctgc aatgtgccct caacaataag atcaatccca
     3841 gtattgcttc ttttgtaatt ccactgtgtg ccaccatcca tctggcgggg agcgccatga
     3901 aaattgttgg attttcgcta gctattatgt atttcttcgg cataccggtc gatccgggca
     3961 cttttatcgg gtttatcttt atggtgggga ttaccatgat tgcggcgccg ggtgttcccg
     4021 gcggagccat tatggcggcc atcgggatta tccagagcat gttgggcttt aacgaacaaa
     4081 tgatcggatt gatgattacc gtttatattg cggtggacag tttcgggact gcctgcaatg
     4141 ttacaggcga cggagccatt gcgctgatta tggataaatg ggcaggaact tcgcggactt
     4201 aaaacggatt atccgctctt gtcccggtgc ctttgcggag tttttctatc tctaagcgga
     4261 gaaccctgtt ctcttccagc atctccaggt atttgttttt atactttacg gagttttctt
     4321 ccaaggtgtc ggttgtttgt tcgacagcgg ctggctgaac cggagtgctt gtcatcttcc
     4381 ctttccccgt gataagccaa tcaaggttta ggtccggaaa agcttccacg atattggaca
     4441 gtttgtccgc accaatcgaa gtttttacct tactgatata tccccgcgaa aaaccaaccc
     4501 gctcttcaaa tttagtttgt cccagtcctt ttgattttat gaataatttt aacctgtcta
     4561 tgaattccat attttgtatt gtttctcagg caattatcaa ttttattatt tagaataaat
     4621 ctaaatcact ccataaatac gaatattgct tgtaatcact gaatatatac agtatatttg
     4681 cattgttaat taatgtttaa tgcgttgcaa caaaattaga catttcacaa agatatgaat
     4741 aatcaggcaa ataccataaa accggttact aaaatatcga tacccgatac actattaagt
     4801 ttaaaggtag gtgaaactgt gatgatatct gcgagaacga ttaattccag ttctgtcagg
     4861 gctgccgcat cccgtttaaa gaaaataaag aaagcacagt ttattgtaac cgaacaaggt
     4921 ttgataaatg aaattaaagt aaccagatta aaataatgaa tatgagtaag ttcaatattt
     4981 tttattgtct ttttcgttct ttgttcatta tctatttatt attgatttgt ggccggtaaa
     5041 catgtccttt caagggttga aagttattct gtaaaattga aattatgtta gcaatatttt
     5101 attaaagcta aatttcactt tatgttaaat tgtttcctgt tcaaaaaaca ttttggttag
     5161 aaatcagtgg ttattgtaaa tgttagctga ggcttccgga gtatgcttat tattaaatgt
     5221 tggttgtgcc tgaacacttt tccactgttc atccgaaaat gaaatatgta gcggctcgtg
     5281 ctttgtccgc acatttgtgt aatttattaa ttctctaaaa ctaatctaag caaaaatgaa
     5341 ggtatgaaaa aacaaaattc agccatgaag tatggaatca tgctcgtctt ttggtggatt
     5401 tgttccgtgg gattgtatgc gcaactgcgt gtgcagggta cggtaaccga tctggatggg
     5461 acccccatga ttggtgtgaa cgtgattgaa aaaggaaccc tgaacggttc agttaccgat
     5521 ctcaacggca actatacgct gacggttgct tctgcagagt cggtgttgca cttctcctat
     5581 gtggggtatg ttgctgaaga tgtaacggta ggtgacaacc ggcagattaa cctggtaatg
     5641 agggaagaag ccgaaagcct ggatgagatt gtagtggttg gctatggcac ccaggtcagg
     5701 aaggatatta caggttcagt agcagtggta aatactgagc aactgctcgc ctctacagga
     5761 tcgtctgctg cgcaacaact acagggtaag acgcccggtg tttacatcgg acaaactggc
     5821 tcgcccggat ccgctactat ggtgcgtatc agaggtgtaa acacgattaa cgacaacggt
     5881 cccctgtatg taattgatgg ggtctcaaca cgcaaccaga acctgagcag ccttaacccg
     5941 aacgatatcg agagcatgca ggtgcttaag gatgcctctt cagcagccat atacggagca
     6001 caggcggcca acggagtgat tttgattact actaagcagg gaaccggatc gggtcagcca
     6061 aagatctctt atgatgctta tttcggatta caaaagaccg gcaaacggta tgatctgctc
     6121 aacgctgctg accgtctggc agtggagtgg gagtcgcaac agaactcatt ctccttgcgc
     6181 ggtgtggaag ggattcccga acatgcacag ttcggtaagg cagtcaaccc cgtcattccg
     6241 aactacctga ctactaccgg tgcagggggg cgtcaggata tcagcttaga cgactattcc
     6301 ttccccaaca accagattgt gccttactcc gataccgatt ggtgggatgc catcgatcgc
     6361 gtggcaccga tgcagaacca tcagctgacc ctcaccggcg gcggtgccaa ggggcgctac
     6421 ctgat
//
LOCUS       DAOSDR010000179         6357 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrimonas sp. isolate AMR_MDS_1826
            NODE_4418_length_6357_cov_4.515868, whole genome shotgun sequence.
ACCESSION   DAOSDR010000179 DAOSDR010000000
VERSION     DAOSDR010000179.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863355
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrimonas sp. (wastewater metagenome)
  ORGANISM  Petrimonas sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Dysgonomonadaceae; Petrimonas.
REFERENCE   1  (bases 1 to 6357)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6357)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:10:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,720
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 35
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6357
                     /organism="Petrimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4418_length_6357_cov_4.515868"
                     /isolate="AMR_MDS_1826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2023866"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..372
                     /locus_tag="PLI30_08010"
     CDS             <1..372
                     /locus_tag="PLI30_08010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008861888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit A"
                     /protein_id="HOI79534.1"
                     /translation="GYIKLGQSSSTLSGGENQRVKLAFYLGEEKSEPTIFIFDEPTTG
                     LHFHDIKTLLKAFNALIERGHTVIIIEHNMEIIKCADHIIDIGPEGGISGGYIVCEGT
                     PETISACKESHTGRFLREKLT"
     gene            complement(378..758)
                     /locus_tag="PLI30_08015"
     CDS             complement(378..758)
                     /locus_tag="PLI30_08015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019538038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2752 domain-containing protein"
                     /protein_id="HOI79535.1"
                     /translation="MTKTGKIVIGAAISLLLVAVAFMFYLFDPEEMAVFPRCPFLLAT
                     GYECPGCGSQRAIHDMLHLDLKNAFSQNALILFLSPYLLFGFYLAFFNGKHRFPKWEK
                     LLFGKWAAIFVISGIMVYWVVRNL"
     gene            complement(765..1337)
                     /locus_tag="PLI30_08020"
     CDS             complement(765..1337)
                     /locus_tag="PLI30_08020"
                     /inference="COORDINATES: protein motif:HMM:NF016394.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GYF domain-containing protein"
                     /protein_id="HOI79536.1"
                     /translation="MNRYFYIDSEGKQKGTFSPEELKSENIKKDTLVWAPGMSEWVRA
                     SNVAELDTLFVDNAGYYPPQPAAQPVTPPAQPLSYGGSLQQQPMPKSWLIESILATVL
                     PFLLCSSIFSLLGIIGIVHASKVESLYARGDYTGSLEASGQAGKWTKIAMWIGIAWVV
                     LWIVAIILIIAFGVSMAGISEVFSGSGYEI"
     gene            complement(1350..1748)
                     /locus_tag="PLI30_08025"
     CDS             complement(1350..1748)
                     /locus_tag="PLI30_08025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI79537.1"
                     /translation="MQNKFHLIFIIGFSLLLLQCSGDRRLYRELVEMADNLNKSVPAQ
                     LDDHTIFLGADVTEGNVFQYRYQLVNTAEPQSMLRAVEEQTKNNIREAFRLNPDLKFF
                     TENEVKIDYIYTDSAGNILKIIHITPEDYK"
     gene            complement(2011..3339)
                     /gene="dgt"
                     /locus_tag="PLI30_08030"
     CDS             complement(2011..3339)
                     /gene="dgt"
                     /locus_tag="PLI30_08030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008772892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008832 - dGTPase activity [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dNTP triphosphohydrolase"
                     /protein_id="HOI79538.1"
                     /translation="MNWERLLSSRRFGMEEYHTENRHDRTEYQRDYDRLIFSSPFRRL
                     QNKTQVFPLPGSVFVHNRLTHSLEVSSVGRSLGENIGRGIRQKYPSSPAHFEETGAIV
                     SAACLAHDLGNPPFGHSGEKAISTFFSEGKGQALQQQVEAEGGRWTDFTCFEGNANAV
                     RLLMHQFKGRRKGGFALTYSTLASIVKYPYSSELSGGKNKFGFFASEEDDYRIIADEL
                     GIPCLGEDPVCYTRHPLVYLVEAADDICYQIMDIEDAHKLHLISTEKAKELFLNFFEG
                     ERRQRRINNLNFVSDINEQIAYLRASVIGLLVNECSEAFLKHEEAILSGEFKGSLIKN
                     ISEKTQRAYRNCSDFAVNNIYLSKDVLDIELAGYRIIGFLLEKLSEAIQNPSHTYSKL
                     LLGRVPEQYEPDSDDLYSKVQSIIDYVSGMTDIYALDLYRKITGMGLPAI"
     gene            3492..5420
                     /locus_tag="PLI30_08035"
     CDS             3492..5420
                     /locus_tag="PLI30_08035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019538044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HOI79539.1"
                     /translation="MTDIIKHECGIAMIRLLKPLDYYYKKYGTWQYGLDKLYLLMEKQ
                     HNRGQEGAGLGAVKLEAAPGNEFIFRERALGSGAISEIFGKVHDAFKDFTPQQLHDIE
                     FVKRSVPFASELFIGHLRYSTTGKSGLTYVHPLLRRNNWASRSLALAGNFNMTNVDEM
                     FEQLISEGQHPRDYADTFVMLESLGHYLDREVQYQYDKIEKSDLNGQQVSHRIEEKLD
                     IPFLLKRASKLWDGGYALCGLIGCGDTFTLRDPWGIRSAFYYFDDEVAVVASERPVIQ
                     TAFNLKKDDVHELQPGEAFIVKRNGTITTQQILEPKEKITPCSFERIYFSRGSDYDIY
                     RERKKLGELLVPEIVETINNDFENTVFSFIPNTAEVAYFGMLEGLEKHFNHNKAVELL
                     EKRDQLTPGEVEMILAKRVRSEKVAIKDIKLRTFIAQGKSRNDLAAHVYDVTYGSLKR
                     GKDTLVIIDDSIVRGTTLKQSIIKILDRLDPKKIVIVSSSPQIRYPDCYGIDMSRMSE
                     FIAFKAAIKLLEERGMQYIIESVYEKCVAQSRKKKEEIVNYVKEIYAPFSDEEISDKI
                     AEMLTDKDIKAEVKIVFQTIDDLHKACPNNAGDWYFSGNYPTPGGNRLVNNAFINYIE
                     GQENKVYQYSLNFTNSVK"
     gene            5417..>6357
                     /locus_tag="PLI30_08040"
     CDS             5417..>6357
                     /locus_tag="PLI30_08040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006841671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HOI79540.1"
                     /translation="MIERILILENVDPVVFFGINNSNVQLLKTLFPKLRIVARGNVIK
                     VIGDEEELVTFEEKVHELEKFSIEMNVLKEEDILDIVKGKAPTVVNLDNLIIHGLNGK
                     PILARTANQKKLVEVFDDNDLVFAIGPAGSGKTYTAIALAVRALKTKQVRKIILSRPA
                     VEAGEKLGFLPGDMKEKIDPYLQPLYDALEDMLPPLKLKEYIENKIIQIAPLAFMRGR
                     TLNDAVIILDEAQNTTKPQIKMFLTRLGLNGKMIVTGDITQIDLPASQQSGLIHSMSL
                     LKGIKGISTVEFNKKDIVRHKLVQRIVEAYEKETPDN"
BASE COUNT         1942 a         1413 c         1363 g         1639 t
ORIGIN      
        1 ggctatatca agctcggaca atcctcttca acattgtcgg gtggagaaaa tcagcgtgta
       61 aaattagcgt tttacctggg tgaagagaaa tcggaaccca ctatttttat ttttgatgag
      121 ccaactactg gactacattt tcacgatatc aaaacactgc tgaaagcatt taatgcactt
      181 atagaaagag ggcatacggt cattattatc gagcacaaca tggaaataat taagtgcgcc
      241 gaccacatca tcgacatagg cccggaaggc ggaatatccg gtggctatat tgtctgtgaa
      301 ggcacgcctg aaacaatttc tgcatgtaag gaatcgcata cgggcaggtt cttacgggag
      361 aagctaacgt agcagattta caaattccta actacccaat ataccattat tcccgatatc
      421 acaaatatag ccgcccattt accaaaaagc agtttctccc atttcgggaa gcggtgtttc
      481 ccattaaaaa aagccaggta aaatccaaac aataaatacg gagacaagaa caggataagt
      541 gcattctgtg aaaaagcgtt ttttaagtcc aggtgcaaca tatcatgaat agcgcgttga
      601 gaaccgcatc cgggacattc atatccggta gccagaagaa acggacaacg gggaaacaca
      661 gccatttcct cggggtcgaa gagataaaac ataaaagcaa ctgccacaag caaaaggcta
      721 atggcagcac ctataactat tttacccgtc ttggtcaatc caaattaaat ctcgtatcca
      781 gatccgctaa acacctcact tatgccggcc atcgacacgc caaaagcgat aatgagaatt
      841 atggcaacaa tccacagaac tacccaggca atacctatcc acatggcaat ttttgtccat
      901 tttccggcct gaccggaagc ttccagcgag ccagtatagt caccgcgggc ataaagagat
      961 tctacttttg aggcatgcac aatccctata attccgagaa gactgaatat actgctgcag
     1021 agtaaaaacg gaagtacagt ggctaagata gattcgatta gccacgattt aggcatcggc
     1081 tgttgttgca gagagcctcc gtaactcaac ggttgggcag gcggggtcac gggttgcgca
     1141 gccggttgag gcggataata acctgcatta tcaacaaaaa gggtatccaa ttcggcaacg
     1201 ttcgaagcac gtacccattc gctcatccct ggcgcccaca ccagcgtatc ctttttgatg
     1261 ttttcgcttt tcaactcttc gggtgaaaat gtgccttttt gttttccttc gctgtcaatg
     1321 taaaagtaac ggttcataat cgtaatgttt tatttgtaat cttcaggtgt aatgtgtatt
     1381 atttttagta tgtttcccgc ggaatcggta taaatataat caattttaac ttcgttttcc
     1441 gtgaaaaatt tcaagtcagg attcaacctg aacgcctccc ggatattgtt tttcgtttgc
     1501 tcttccacag cccggagcat cgactgaggt tcagctgtat tcaccagttg ataccggtac
     1561 tgaaaaacat tgccttccgt tacatctgcc cccagaaaaa tggtatggtc atcgagttga
     1621 gccggtacgg acttgttgag gttatcagcc atttccacca gttcccgata caatcttctg
     1681 tcgcctgaac attgtaaaag tagcagggaa aacccaatta taaaaatgag gtgaaattta
     1741 ttctgcatga cgtaaaatct tgatacaaaa ttaaaaaagt ttgcgtaggg tagaaaatat
     1801 tttgtaatta ttttgtggaa gtttatgaat atccccctaa aaaagatttt ggcaggatat
     1861 ctcgcgacat cctgccaaac atgttaacct taaatctaat actattatga aaaaaccacg
     1921 ttgcaaatat aaaaaataaa ttaaaagcct ccaaattttt tcaataaaaa attaaaaaat
     1981 atttttcgct tacaatttga cactaattaa ttatattgcc ggcaacccca ttcccgtaat
     2041 tttccggtat aagtcgagtg cgtagatgtc ggtcattccg gatacgtaat caatgattga
     2101 ctgtacttta gaatagagat catccgaatc cggctcatat tgttcaggga ctcttcccag
     2161 caacagcttt gaatacgtat gagaaggatt ctgtatggcc tccgacaatt tttcgagcaa
     2221 aaatccgata attctgtatc cggccaactc aatatccagt acatcttttg acaaataaat
     2281 attatttacg gcaaaatcgg aacagttcct gtatgctctt tgtgtttttt cggatatatt
     2341 ttttattaac gagcccttaa attctcccga caagatagct tcttcgtgtt ttaaaaatgc
     2401 ttccgaacat tcattcacca acaatccgat gacacttgcg cgcagataag cgatctgttc
     2461 gttaatatca ctaacaaaat tcaaattatt gattctgcgt tgtcgtcttt cgccttcaaa
     2521 aaaattgaga aaaagctctt tggctttttc ggtagaaatc aaatgcagtt tatgggcatc
     2581 ttcaatatcc atgatctggt agcaaatatc atctgcggct tccaccagat agaccaacgg
     2641 atgacgcgta taacaaaccg gatcctctcc taggcaggga ataccgagtt catcggcaat
     2701 aatcctgtaa tcgtcttcct ccgaagcaaa aaaaccaaac ttgttttttc cgccgctcag
     2761 ctccgatgaa tagggatact taacaatgga tgccagggtg gaataagtca gagcaaaacc
     2821 acctttacgg cgtcctttaa actgatgcat caataaacga acggcattgg cgtttccttc
     2881 aaaacaggta aaatcggtcc accgtccccc ttcggcttcc acttgctgct gtaacgcctg
     2941 tcctttacct tcggagaaaa aagtagaaat ggctttttca cccgagtgcc caaacggagg
     3001 gtttcccaga tcatgcgcca gacaagcagc ggaaacgata gctcccgtct cttcaaagtg
     3061 agccggcgaa gagggatatt tctggcgaat tccacggccg atattctctc ccagtgaacg
     3121 cccgacactc gacacttcaa ggctgtgggt taaccggttg tgaacaaaca cgcttccggg
     3181 aagggggaag acctgcgttt tgttttgcaa acgtctgaaa ggtgatgaga aaatcaaccg
     3241 gtcataatcg cgttgatact ccgtcctgtc gtgcctgttt tcggtgtgat attcctccat
     3301 cccgaatcgc ctcgaagaca ataatcgttc ccaattcatt ctaaaatcat acttttacta
     3361 caaagataag ataaagtttt ttagtaacat cgttagctgc acgccactca aaacaaaaat
     3421 taaccccaaa catcatcatt ttctccccaa atacccttaa atttgtgcct taatttcaga
     3481 aaggtttatc aatgactgac atcatcaagc acgaatgcgg tattgcaatg atccgcctac
     3541 taaaacccct cgattattat tacaaaaaat acggaacgtg gcagtacggt ctggacaagc
     3601 tttacttgct gatggaaaaa cagcacaacc gtgggcagga aggtgccggc ctgggtgccg
     3661 taaaactgga ggccgctccc ggtaatgaat ttattttcag ggaaagggcg ctgggatcgg
     3721 gtgctatctc agagattttt gggaaagtac acgatgcatt caaggatttt actccacagc
     3781 aactccacga tatcgaattc gtaaaacgtt cggttccgtt tgcttccgaa ttatttatcg
     3841 ggcatcttcg ttacagcacc accgggaaaa gcggcttgac gtatgttcac ccgctgctgc
     3901 gccggaacaa ctgggcttcg cgcagtctgg cgctcgccgg aaacttcaac atgacgaacg
     3961 tggacgaaat gttcgaacag ttgatttcgg aaggacaaca tccgcgcgat tatgccgata
     4021 catttgtgat gctggaatca ctcgggcatt acctcgacag ggaggtgcaa taccaatacg
     4081 acaaaataga aaaatcggat ctgaacggcc agcaggtaag ccaccgtatc gaagaaaaac
     4141 tggacattcc gtttcttctg aagcgtgcca gcaaattatg ggacggcgga tatgcgcttt
     4201 gtggacttat cggctgcggc gacacgttca ccctccgtga tccgtgggga attcgttcag
     4261 ctttttacta ttttgacgat gaagtagcgg tggtagcatc ggaacgcccg gtcatccaaa
     4321 cggcgttcaa cctcaagaaa gatgatgttc acgaattaca acccggtgaa gcctttatcg
     4381 tgaaacgaaa cggaacgatc acaacccaac agattctgga accaaaagaa aaaataaccc
     4441 cctgttcgtt tgaacggatc tatttttcac gtggctcgga ttatgatatt taccgcgaaa
     4501 ggaagaaact tggcgaactt cttgttcccg aaattgttga gacaataaac aatgattttg
     4561 aaaacacggt tttctcgttc atccccaaca ccgccgaagt agcatacttc gggatgctcg
     4621 aaggattgga aaaacatttc aaccacaaca aggcggttga gttactcgag aaacgtgacc
     4681 aattgactcc cggcgaagtc gaaatgatcc tggccaaaag ggtacgctcg gaaaaagtcg
     4741 cgataaaaga cataaagttg cgtacattca ttgcacaagg aaaatccaga aatgacctgg
     4801 cagcccatgt ttacgacgtc acgtatggtt cactgaagcg cggaaaggac acgttggtta
     4861 ttattgatga tagtatcgtg cgcggaacga cactgaaaca aagtattatc aagattctgg
     4921 acaggctgga cccgaagaag atcgttattg tttcttcatc tccgcaaatc cgttatcccg
     4981 attgctacgg tatcgacatg tcgcgcatga gcgaattcat tgctttcaaa gcggcaatca
     5041 aactattgga agaacggggt atgcagtata ttatagaaag tgtatatgaa aaatgtgttg
     5101 ctcagagccg gaagaaaaaa gaagaaatcg tgaattatgt aaaagagatt tatgcccctt
     5161 tttcggatga agaaatttcg gataagattg ccgaaatgct taccgacaag gacataaagg
     5221 ctgaagtaaa aattgtgttt caaaccatcg acgacttgca caaagcgtgt cccaacaacg
     5281 ctggcgactg gtatttttcg ggcaattatc ctactccggg cggaaacaga ttggtcaata
     5341 atgcgtttat caattatata gaaggacagg agaacaaagt gtatcaatat tcgttgaact
     5401 ttacaaacag cgttaaatga tcgaacgtat cctcattctc gaaaatgtag acccggttgt
     5461 attttttggg ataaacaaca gtaacgtcca gttactgaaa acgttgtttc caaaacttcg
     5521 tattgtggcc cgcggcaatg taatcaaagt tattggcgat gaggaggaac tggttacctt
     5581 cgaagaaaag gttcacgagt tggagaaatt cagcatcgag atgaacgtgc tgaaagagga
     5641 agacatcctg gatatagtga aagggaaagc accaacggtt gtgaacctgg ataacctgat
     5701 cattcacggg ctcaacggaa aaccgatctt ggcgcgtacg gcaaaccaaa aaaaactggt
     5761 ggaggtgttc gacgataacg acctggtctt cgccatcggc cctgccgggt cagggaaaac
     5821 atataccgct attgcactgg ctgtaagagc gttaaaaacc aaacaagtca gaaaaataat
     5881 actcagccgc ccggccgttg aagccggtga aaagctgggt ttccttccgg gtgatatgaa
     5941 agaaaagatc gatccctatc ttcaacccct ttatgacgca ctggaagata tgcttcctcc
     6001 attgaaactc aaagaataca tcgaaaacaa gattattcag attgcaccac ttgcctttat
     6061 gcgtggacgc acgctgaatg atgcggtcat tattctcgac gaagctcaga atacgaccaa
     6121 accgcagata aagatgttcc ttacccggct ggggctgaat ggaaaaatga ttgttacggg
     6181 agatattacg caaatcgatt tgcccgcgtc tcaacaatcg ggactaattc attcaatgag
     6241 tctgctgaaa ggtatcaaag gaatatccac cgtggagttc aataaaaaag atatcgtccg
     6301 ccacaaactc gtacagcgga ttgtagaggc ctacgagaaa gaaacgccgg acaattt
//
LOCUS       DAOSDR010000180         6349 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrimonas sp. isolate AMR_MDS_1826
            NODE_4424_length_6349_cov_3.556085, whole genome shotgun sequence.
ACCESSION   DAOSDR010000180 DAOSDR010000000
VERSION     DAOSDR010000180.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863355
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrimonas sp. (wastewater metagenome)
  ORGANISM  Petrimonas sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Dysgonomonadaceae; Petrimonas.
REFERENCE   1  (bases 1 to 6349)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6349)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:10:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,720
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 35
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6349
                     /organism="Petrimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4424_length_6349_cov_3.556085"
                     /isolate="AMR_MDS_1826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2023866"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..228
                     /locus_tag="PLI30_08045"
     CDS             <1..228
                     /locus_tag="PLI30_08045"
                     /inference="COORDINATES: protein motif:HMM:NF036806.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI79541.1"
                     /translation="WEMTTPGQPVQQQLFVELSTEEQQIGKVLRNNGKMFIDLICQET
                     GLPMGKVSALLLELEFRGVVDALPGKMYRLR"
     gene            280..1590
                     /locus_tag="PLI30_08050"
     CDS             280..1590
                     /locus_tag="PLI30_08050"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HOI79542.1"
                     /translation="MKTFFDLKISKAILKAVEEIGFTEPTPIQEQVIPIINSGVNVVG
                     VAQTGTGKTAAYLLPLLTKLVRPEGNDPRVVILVPTRELSLQVGEDIEELTAFSEIRF
                     ASVFGGVGWTKHAALLEPGNDILVATPGRLWDLYQAGAVSFKKVKFLVIDEADRMLDM
                     GFMPQLRQLFEILPPRRQNLLFSATFSEKVETMAEEFLDHYEKIEVAPSATPVELVEQ
                     VVYHVPNYRTKLNLIEHLLQDEAAYNRVILFVKTKEHAEDVYKVIKRKAEGEKRIMHS
                     NKGQSTRLNAIRAFKEGEVRILITTDLSARGLDASLVSHVINFDIPPQYEDYVHRIGR
                     TARAGNRGTAITLAAPDEEWHLKRIEQLIRMKVPVAEIPAEVKIVETEFEERQQQLRE
                     IDRQRRIDDPTYQGAFHQRKRKTEKFKKPSAAKSASPARKRRRS"
     gene            1659..2645
                     /locus_tag="PLI30_08055"
     CDS             1659..2645
                     /locus_tag="PLI30_08055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018339456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOI79543.1"
                     /translation="MATAEQIKSLIQSHFLGSDERFATIALQIAAHEARAGRLNVAEE
                     IKQTIDRSKSKIRILNPLQKDLQGLLIESSSERRMNELVISDEIKKRLERILTEYKQR
                     HKLQKFGLSHRRKILLAGPPGTGKTMTASVLANEMHLPLFTVSMDKMVTKYMGETGAK
                     LRLIFDAIRERNGIYLFDEFDAIGAERGNENEVGEMRRVLNAFLQFIENDFSESLIIA
                     ATNNLSVLDPALFRRFDDVIYFKNPDKTEATLLLKNLLGSFIGTIKPETIAKEIEGLS
                     HAEISLGCIDAIKDAILADKEVVDKKTLLSMIQDRHHIHSKTNLNGQTDPIS"
     gene            2620..5109
                     /locus_tag="PLI30_08060"
     CDS             2620..5109
                     /locus_tag="PLI30_08060"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family peptidase"
                     /protein_id="HOI79544.1"
                     /translation="MDKPILFLNDAAQSESFTTTKSGGGELRLPYRDRSAHAASLASK
                     FNTARSTWGEQQQQRKALSLPSRDGCYLEILGKAGYELITNSLEDRRSKKTLGARLIS
                     VVTGDNQEKRAIVYIPYRQENKFLKKIQDYADSTKDKENKPQNKKQIESIEDIKLALL
                     EDLWQDPKDSLPQEIPVWCELWLRTENIDADEVITKTQKICTAIGIETNKERLDFPER
                     SVIAIRANREQLQELYAQSDNLAELRLAKEPVSFWSSMSNFEQTEAVDDLLDRVIADD
                     KGVSVCILDSGINNGHRLLELFCSDHEKETYHPEWNPNDQEGHGTQMAGIAAFGDLQE
                     ALESRDSVTINHRLFSGKILPPPNFPENDPKLYGYITAQTISNCEIKGQVRKQLYCLA
                     VTTLHDIEREGRPTSWSSEIDKLAFGEEDGFQRLILISAGNAKNLVDLQDLKKYPEIN
                     KELPVQNPAQAWNALTIGAYTEKVRITDPDYRDYNPLAQHGELSPHSTTSLTWDTKRW
                     PNKPDIVLEGGNLLKTPDGTVDGCDDLCVLTTSHRPFGKQFELFDATSAATAQAAWMA
                     AQIQSIVPNAWPETIRALMVHSAEWTPQLIAQFGIDLKKKGDIGNLLRIAGYGVPDLK
                     KASECATNHLTLIAQEYIQPFKKEKGLVKTNIMHFYELPWPEEILRQIGDQSVTLKIT
                     LSYFIEPSPGELQTQNRYTYASHALRFDINSSQEKDNIEEFKKRINKQSWEDENDRSE
                     TKGFREEWKIGTTYRNKGTVQSDSITLSGIEIADCNHIAVYPIGGWWKTRTSEKCWEK
                     QARYSLVVSLHTESEEIDIYTPVALKVMVEI"
     gene            complement(5124..6209)
                     /locus_tag="PLI30_08065"
     CDS             complement(5124..6209)
                     /locus_tag="PLI30_08065"
                     /inference="COORDINATES: protein motif:HMM:NF014979.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HOI79545.1"
                     /translation="MPEKLKDILFPRHKVEYFAQILQEAYPPFESKNFVDCVCDKRWP
                     ERELKEKMRHTTLCLHRFLPEDFRQSVEILKALVPKVKGFEAIVLPDYVEVYGLEHWD
                     ISLPALGILTKCGSSEFAIRPFLNKNTAGAMQFMQRWAADPDPRVRRLASEGCRPRLP
                     WGMAVPALKEDPSPILPILEKLKNDPAEAVRRSVANNLNDISKDHPELVLGLCERWAG
                     ESQESDRIIKHACRSLLKEGNKRAMLLFGFANPEKMQISEIKVSSGKLKIGGEINLSF
                     ELLLQTEKPQMVRLEYVVHYVKANGKTSPKVFQIREAELAPGTHRIRKKHAFADVSVR
                     KHYPGVHSIEVVVNGEVKAAVSVQLEE"
BASE COUNT         1812 a         1428 c         1522 g         1587 t
ORIGIN      
        1 tgggaaatga caacgcccgg gcagcctgtt cagcagcagc tttttgttga actctcgacc
       61 gaagaacagc agatcggtaa agtgctccgc aacaacggga aaatgtttat cgacctgatt
      121 tgccaggaga ccggtttgcc gatgggaaaa gtgtcggcgc tgttgctgga gctggagttt
      181 cgcggggtgg tcgatgcgct ccccggaaaa atgtaccggc tgcgatgagc tgaaactgcc
      241 ggaagtctct tcttttcaaa gttttcgtac ttttgcggca tgaaaacatt tttcgatctc
      301 aaaatttcca aagccatatt aaaagccgtc gaagagattg gctttaccga gccaacgccg
      361 attcaggaac aggttattcc gatcatcaac tccggtgtaa acgtggttgg ggttgcccaa
      421 accggtaccg gaaaaacggc agcttacctg ttgccgctgc ttaccaaact ggttagaccc
      481 gaagggaatg acccccgtgt cgtcattttg gttcctaccc gcgaactctc cctgcaggtg
      541 ggggaagata ttgaagagct gacggccttt tccgaaattc gttttgcctc ggtgtttggc
      601 ggggtgggct ggaccaaaca tgctgccctg ctggaacccg gcaatgatat tctggtcgcc
      661 acgcccgggc ggttgtggga tttgtaccag gctggcgccg tatcgtttaa aaaagtgaaa
      721 tttctggtga tcgacgaggc cgaccggatg ctggacatgg gatttatgcc gcagttgcgc
      781 cagcttttcg agattcttcc gccccgcagg caaaacctgc tgttcagcgc cactttttcg
      841 gagaaggtgg agacaatggc ggaggagttc ctcgatcatt acgaaaagat tgaggtggcg
      901 ccatcggcta caccggtcga actggtggag caggtggttt accacgtccc gaattaccgg
      961 accaaactca acctgattga gcacctgctg caggacgaag cggcctacaa ccgggtgatc
     1021 ctgttcgtaa aaaccaaaga acacgccgaa gatgtttata aagtgatcaa acggaaagcc
     1081 gaaggtgaga agcggattat gcactccaac aaaggacaga gtacccgtct gaatgccatc
     1141 cgcgccttca aggaggggga agtccggatt ttgatcacca ccgatttgtc ggcccgcggc
     1201 ctcgatgcca gcctggtgag ccatgtgatc aattttgata ttccgccgca gtacgaagat
     1261 tatgtgcacc gcattggccg cactgcacgc gccggaaacc gcgggacggc catcaccctg
     1321 gctgcccccg acgaagagtg gcacctgaag cggatcgagc agcttatccg gatgaaggta
     1381 ccggtggccg aaattccggc agaggtaaaa attgtggaaa ccgagtttga ggaacggcag
     1441 cagcagctgc gtgaaatcga ccgccagcgc cgcatcgacg accctaccta ccagggcgct
     1501 tttcaccaga gaaaaaggaa aaccgaaaaa ttcaaaaaac catcagccgc gaaatccgct
     1561 tctcccgcca ggaagagacg gagaagctaa agaataagat tttttgaggt gagggttgaa
     1621 gtatttggat gtttaatttt tattaaattt gtttatctat ggcaacagca gaacagataa
     1681 aatcgttaat ccaatcgcat tttttaggta gcgatgaacg ttttgcgacg attgctctac
     1741 aaatagccgc tcacgaagcc cgtgccggac ggttgaacgt agcagaagag atcaaacaaa
     1801 ccattgaccg atcgaaatcg aagatccgaa ttctcaaccc acttcagaag gatttacaag
     1861 gactactcat cgaatcttct tctgaaagga gaatgaacga gttggtaatt tctgatgaaa
     1921 ttaaaaaacg gttggagcgg attcttaccg aatacaagca acgccacaag ttgcaaaagt
     1981 ttggtctttc acatcgcaga aagatcttgc tggccggccc tccgggtaca ggcaaaacca
     2041 tgacagcttc ggttttggcc aacgaaatgc acctgccact cttcaccgtt tcgatggata
     2101 aaatggtcac caaatacatg ggtgaaacgg gagccaaact ccggttgatt tttgatgcca
     2161 taagggagcg caacggtatt tatcttttcg atgagtttga tgctattgga gccgaacgag
     2221 gaaatgaaaa cgaggtgggc gaaatgcgcc gtgtgcttaa tgctttcctt cagtttattg
     2281 aaaacgactt ttcggaaagc ttgattattg ccgccaccaa caacctttcg gttctcgacc
     2341 cggctttgtt ccgacgtttt gacgatgtga tctactttaa aaatcctgat aaaacagaag
     2401 ccacacttct actgaagaat cttttaggct catttattgg aaccatcaaa cccgaaacca
     2461 ttgccaaaga aatcgaaggt ttaagtcacg ccgaaattag cttgggctgc atcgatgcca
     2521 ttaaagatgc catccttgcc gataaagagg tggtggataa aaaaacactt ctgtccatga
     2581 ttcaggatcg acatcacatc cactcaaaaa cgaatctaaa tggacaaacc gatcctattt
     2641 cttaacgacg cagcacaatc cgaatcattt actacaacaa aaagtggtgg tggagaactt
     2701 aggcttcctt accgtgatag atcggcccat gctgctagct tggcttcaaa gtttaatacc
     2761 gctaggagta cctggggaga acaacagcaa caaaggaagg ctctttcttt gcccagtcgc
     2821 gatggttgtt atcttgaaat tttgggcaaa gcaggttatg aactcataac aaatagcctt
     2881 gaagatagaa gaagtaagaa gacacttgga gcaagactga tttcggttgt aacaggtgac
     2941 aatcaagaga aacgagcaat agtttatatc ccttataggc aagagaataa gttcttaaaa
     3001 aagattcagg attatgctga ttccactaaa gacaaagaga acaaacccca gaacaagaag
     3061 caaattgaaa gtattgaaga tataaaactt gcccttttag aagacttatg gcaagacccg
     3121 aaagatagtc ttccacaaga aattccggtt tggtgtgaat tatggcttcg tactgagaac
     3181 atagatgctg atgaagttat cacaaaaaca cagaagatat gcacagctat tggtattgaa
     3241 accaataaag aacgccttga ttttcctgaa cgatctgtta ttgcaatacg cgcaaatcgg
     3301 gaacaacttc aagagcttta tgcgcaaagc gacaatttgg ctgaactacg tttggccaaa
     3361 gagcccgttt ctttctggtc gtcgatgtcg aattttgaac aaacagaagc cgttgatgat
     3421 cttttggatc gggttatagc tgatgacaag ggggtaagtg tgtgcatatt ggatagcgga
     3481 attaacaacg gccatcggtt gctggaacta ttttgctccg atcatgaaaa ggaaacctat
     3541 catccagaat ggaatccaaa tgatcaagag ggacatggaa cacagatggc aggaattgca
     3601 gcatttggcg atttacaaga ggcattagaa tcacgtgatt ctgttaccat caatcatcgt
     3661 ttgttttcgg gcaaaattct tccaccacca aattttcctg agaatgatcc aaaactctat
     3721 ggatacatta ctgctcagac gataagtaat tgtgaaataa aaggacaggt gcgtaagcaa
     3781 ctttattgct tggctgtaac tactcttcac gatattgaga gagaaggaag accaacatca
     3841 tggtcatcag aaattgacaa actggcattt ggtgaagaag atggatttca acgattaatc
     3901 ctcataagtg ctgggaatgc gaaaaatcta gttgaccttc aagatttaaa aaaatatcct
     3961 gagataaata aagaactacc agtccaaaat ccggcacaag catggaatgc tttaaccatt
     4021 ggagcttata ccgagaaagt aagaatcact gaccctgatt atcgggacta caatccactt
     4081 gcccagcatg gagagctttc tccgcacagt acaacttctc tgacttggga tacaaaacgc
     4141 tggcctaata aacccgatat tgtattggaa ggtggcaacc tacttaagac gccagacgga
     4201 acagttgatg gttgcgacga tttatgtgtc ttgaccactt cacacagacc atttggtaaa
     4261 cagtttgagt tgtttgatgc cactagtgcc gctacagccc aagcagcatg gatggcagct
     4321 caaattcaaa gcattgtgcc caacgcttgg cctgaaacca ttcgcgcgtt aatggttcac
     4381 tctgctgaat ggacacctca acttattgct caatttggaa tagatctaaa aaagaaagga
     4441 gatattggca acttgttgcg gatagcaggc tatggagttc ctgatttgaa gaaggcttcg
     4501 gaatgtgcaa caaatcattt aacgctaatt gctcaagagt atatccaacc gtttaaaaag
     4561 gaaaaaggtc tggtaaaaac caatatcatg cacttttacg aacttccctg gccagaggaa
     4621 atcctaaggc aaattggaga ccaatcggtt acacttaaaa ttactctttc ctattttatt
     4681 gaaccttctc ccggtgaact tcaaacacaa aacagatata cctatgcatc acacgctttg
     4741 aggtttgata taaattcctc tcaagaaaaa gataatattg aggaattcaa aaagcgaatc
     4801 aataaacaat cttgggaaga cgaaaatgat agatctgaaa cgaaaggttt tcgtgaagaa
     4861 tggaagatag gaactaccta tcgtaacaaa ggaacagttc aatccgactc gattacttta
     4921 tctggaattg aaattgcaga ctgtaatcac atagctgttt acccaattgg tggttggtgg
     4981 aaaacccgaa cgagtgaaaa atgttgggag aaacaagccc gttattcatt ggttgtttca
     5041 ttacatacag aatcggaaga gatcgatatt tatactccag tggccttaaa ggtaatggtg
     5101 gaaatataat caatgttata attttattct tccagctgaa cggaaaccgc agctttaacc
     5161 tcgccgttaa ccaccacttc gatgctgtgg acgccgggat agtgtttccg gacagagacg
     5221 tcggcaaaag cgtgtttttt ccggatgcgg tgggtgcccg gcgccagctc tgcctccctg
     5281 atctgaaaaa cttttggcga ggttttgccg ttggccttta cgtaatgcac cacatattcg
     5341 agccgcacca tctggggttt ttctgtctgc agcaacagtt cgaacgacag gttaatctcc
     5401 ccgccgattt tcagcttgcc ggacgagact tttatttcac tgatctgcat cttctcggga
     5461 ttggcaaagc caaacagcag catggcccgt ttgtttccct ccttcaggag tgaacggcag
     5521 gcgtgtttga tgatacggtc gctctcctgt gactcgccgg cccaccgctc gcagagaccg
     5581 agcaccagct ccgggtggtc ttttgagatg tcattcaggt tgttggccac actgcgacgg
     5641 accgcttccg ccgggtcatt tttcagtttt tcgaggatgg gcagaatggg cgaagggtct
     5701 tctttcaggg ccggaacagc cattccccac ggcagccgcg ggcgacaacc ttcactggcc
     5761 agccggcgca cgcgcggatc gggatctgcc gcccaacgtt gcataaactg catcgcaccg
     5821 gcggtgtttt tgttgagaaa cggccggatg gcaaactccg aggagccgca tttggttagt
     5881 atacccaacg ccggcagcga aatatcccag tgctccagtc cgtaaacttc aacataatcg
     5941 ggcaggacga ttgcttcaaa accttttact ttcgggacaa gtgccttcag gatttcaacg
     6001 gattgccgga aatcttccgg taaaaaccgg tgaaggcaaa gggtggtatg acgcatcttt
     6061 tctttcagct cccgttccgg ccatcttttg tcgcatacgc agtcaacaaa attttttgat
     6121 tcgaagggcg gatacgcctc ttgcaggatc tgggcaaaat attccacctt gtggcgcggg
     6181 aaaagaatat ctttcagttt ttcaggcatg gttccaacgt tttgccgggt tgcttttcgg
     6241 tagaagtaaa tttaccggat attctcgtcc aaccaattgg tgatgacctg ttttttttgc
     6301 gttaattcgt tgaaaagcgc agtgttgcaa cgtttgattt tcctccagc
//
LOCUS       DAOSDR010000181         6341 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrimonas sp. isolate AMR_MDS_1826
            NODE_4434_length_6341_cov_4.129017, whole genome shotgun sequence.
ACCESSION   DAOSDR010000181 DAOSDR010000000
VERSION     DAOSDR010000181.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863355
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrimonas sp. (wastewater metagenome)
  ORGANISM  Petrimonas sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Dysgonomonadaceae; Petrimonas.
REFERENCE   1  (bases 1 to 6341)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6341)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:10:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,720
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 35
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6341
                     /organism="Petrimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4434_length_6341_cov_4.129017"
                     /isolate="AMR_MDS_1826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2023866"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..397)
                     /gene="fucI"
                     /locus_tag="PLI30_08070"
     CDS             complement(<1..397)
                     /gene="fucI"
                     /locus_tag="PLI30_08070"
                     /EC_number="5.3.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010991938.1"
                     /note="catalyzes the conversion of the aldose L-fucose
                     into the corresponding ketose L-fuculose; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose isomerase"
                     /protein_id="HOI79546.1"
                     /translation="MKTYPKIGIRPAIDGRQGGVRESLEEKTMNLAKAVANLISSTLK
                     NGDGSPVECVIADTTIGRVGESAACAEKFEREGVGSTITVTSCWCYGSETMDMNPHYP
                     KAVWGFNGTERPGAVYLAAVLAAHAQKGLP"
     gene            complement(477..1460)
                     /locus_tag="PLI30_08075"
     CDS             complement(477..1460)
                     /locus_tag="PLI30_08075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002558714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HOI79547.1"
                     /translation="MELEFNNKNTKVKQLADYIQRAISMNELKPGDKLSSINTLSRQH
                     NISRDTVFKAFNDLRARGLIESIHGKNYYVSYRVKNVLLILDEYTPFKDVLYNALVSK
                     LPSNHKIDLWFHQYNENLFNNIVRESYGMYNKYLIMNYSNDVLAESLSKINPKKLLLL
                     DFGSFNKEGLSYVCQDFDRAFYDALDQAKLDFKKYKKIVFLFDKRHKHPQSSKDYFIR
                     FCTDNNFEFEIIESFTPQTPVVEGTLYIIIKQTDVVEVIKRSKADKLTVGRQFGIIGY
                     NNNPFYEVIENGIAAISIDWEEMGNLAADFVITGNPITRYLPTKIIKRGSY"
     gene            complement(1682..2419)
                     /locus_tag="PLI30_08080"
     CDS             complement(1682..2419)
                     /locus_tag="PLI30_08080"
                     /inference="COORDINATES: protein motif:HMM:NF036640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NigD-like protein"
                     /protein_id="HOI79548.1"
                     /translation="MKDTAWGFVFFIVLLTVASCNKKDEHSLGEFHIDIATVIPMGEN
                     GYMLMLDNGKQLWPAATDVHYLPVGNYQRVFVNYTTLSDEQGGYDHYVKVNDIWNILT
                     KKVIEQTSQNADSIGHDPVKINEAWVGNDYLNIDFMFNYGGVRPHAINLVIDSLHPDK
                     APDTLELEFRHNAYGSSSPKFFEGFICFDLKPLQRADTDSVQLTVKAKDEDGEKIFNV
                     VYRYNQAALQNKIAETPIPVVASNEYY"
     gene            complement(2501..4339)
                     /locus_tag="PLI30_08085"
     CDS             complement(2501..4339)
                     /locus_tag="PLI30_08085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004325559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YgiQ family radical SAM protein"
                     /protein_id="HOI79549.1"
                     /translation="MESQPYPSAHFLPTTKKEMDLLGWDQADVILFSGDAYIDHPSFG
                     TAVIGRLLERLGLRVAIVPQPNWRDDLRDFKKLGLPRMFFAVTAGVMDSMINRYTANK
                     RLRSQDAYTPDGRSDMRPDRAVTVYSNILKELYPDIPLIIGGVEASLRRVSHYDYWSD
                     SLHKTILAGTNADLLVYGMGELPITRVIREMQNGKTIAEMCHVPQTAFLAGKDNVPEN
                     SFKDEIRLFSHEECLEDRLKQAKNFRIVEEQSNALHASRILQDVDNKTLVVNPPFPPM
                     TEEEIDASFDLPYTRLPHPKYKNKHIAAYEMIKFSVNLHRGCFGGCAFCTISAHQGKF
                     IASRSKESILREVGKITEMPGFKGYISDLGGPSANMYKMQGKDLSVCEKCRRPSCIHP
                     GICPNLNTNHAPLLDIYRSVDNLPMVKKSFIGSGIRYDMLLHRGKDESANKAAEEYTK
                     ELIVNHVSGRLKVAPEHTADRTLNVMRKPSFGLFYQFKKIFDAENNKQNLNQQLIPYF
                     ISSHPGCTEEDMAELAAITKNLNFKLEQIQDFTPSPMTLATEIYYTGYHPYSLEKVYT
                     AKSPSQKESQRQFFFWYDKNNRKKLIAELKRLNRTDLIEKLYPKVK"
     gene            complement(4343..6073)
                     /gene="sulP"
                     /locus_tag="PLI30_08090"
     CDS             complement(4343..6073)
                     /gene="sulP"
                     /locus_tag="PLI30_08090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006799461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015116 - sulfate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate permease"
                     /protein_id="HOI79550.1"
                     /translation="MQKIFLKEFKPILFLSLKNYNREKFISDLMAGLIVGIVALPLAI
                     AFGISSGVTPEKGIVTAIIAGFIISFLGGSHVQIGGPTGAFIVIVYGIVEQFGVTGLA
                     IATVLAGAMLVLMGVLKLGTVIKFIPYPIVVGFTSGIALTIFSTQIKDLFGLSIAKVP
                     SDFFTKWEVYFQHLETINWWATGIGVLSVTIIFLTPKISRKIPGSLIAIVLMTVIVFV
                     MKHYFGITGIETIGDRFQINSQLPQANPIGFNLESIRMLFPSAFTIAILGAIESLLSA
                     TVADGVIGKRHNSNMELVAQGVANIVTPFFGGIPATGAIARTMTNINNGGRSPVAGVI
                     HAVVLLLILLFLGDLTKHIPMACLAGVLVVVAYNMSEWRTFRSLVKQSGGTLAVLLTT
                     FILTVVFDLTIAIEVGLLLAIFLFLKRMNESTQVSVTTGKIDISKEIESHSGNLQDEV
                     LHLPKGVEVYEIDGPFFFGVANKFDDVMREIGERAQIRIIRMRKVPFIDATGLNNLQN
                     LCKNSRREKIQVILSGVNGNVRAKVENSKIPDIIGKENICSNIHLAVDRAIVLSEKMG
                     EERHRKHKLF"
     gene            complement(6143..>6341)
                     /locus_tag="PLI30_08095"
     CDS             complement(6143..>6341)
                     /locus_tag="PLI30_08095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019538410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4294 domain-containing protein"
                     /protein_id="HOI79551.1"
                     /translation="LMKLVDRETNSSSYHIIDAYMGSAKAFFYNAFASMFGNSLKKRY
                     NPYAEDRLTERACVLVEQGAF"
BASE COUNT         1591 a         1453 c         1445 g         1852 t
ORIGIN      
        1 caggcaaccc tttttgggcg tgagccgcca gaacggctgc caggtacacc gctcccggac
       61 gttcagttcc gttgaaaccc catactgcct tggggtaatg cgggttcatg tccatggttt
      121 cggatccgta gcaccagcac gatgtaacag ttatggtgga tcctacgccc tcacgttcaa
      181 atttttcggc acaggcagca ctttcgccca cccgtccaat agttgtgtcg gcgattacac
      241 attcaacggg acttccatca ccgtttttta aggttgaact tatcaggttg gcaacggcct
      301 ttgccaggtt cattgttttt tcctcgaggc tctctctgac gcctccttgt ctcccgtcga
      361 tggcgggacg aataccgatt tttggataag ttttcatagc gtttttattt ttttaatgat
      421 tcgtttgtgc aaatttaagt tgtagtatga acagtgctga tgttgcagga aaaaaattaa
      481 taggaccctc tcttgatgat ttttgtcgga agataacggg taatgggatt gccggttatc
      541 acgaaatcgg cagctaggtt gcccatctcc tcccagtcga tgctgatggc cgcaattccg
      601 ttttcaatca cttcgtaaaa agggttgttg ttataaccga taatcccgaa ctgtctgccc
      661 acggtcagct tgtcggcttt gctccttttg atgacctcta ccacatcggt ctgcttgata
      721 atgatgtata atgtgccctc cacaacaggc gtttgaggtg tgaaagattc gatgatttca
      781 aattcgaaat tgttgtccgt acagaaccgg ataaaatagt ctttgctgct ttgcggatgc
      841 ttgtgtcgtt tatcgaacag gaaaacaatt tttttgtatt ttttgaaatc aagctttgct
      901 tgatccaagg catcgtaaaa agcccggtca aagtcctggc agacgtacga cagtccttcc
      961 ttgttaaaac tgccgaaatc gagcagcagt aattttttgg gattgatttt tgacaacgac
     1021 tctgccagca catcgttgct ataattcata atcaggtatt tgttgtacat gccgtaagat
     1081 tcccgaacaa tgttgttgaa aaggttctcg ttgtattgat ggaaccagag gtcgatttta
     1141 tggtttgaag gcagcttgga taccagcgca ttatacaaca cgtctttgaa cggtgtgtat
     1201 tcatcaagga tgagcagcac gttttttacc cggtagctta cgtagtagtt tttcccgtgg
     1261 atggattcga tcagcccacg tgctctcagg tcattgaagg ctttgaaaac ggtatcgcgg
     1321 gatatgttgt gctgtcggct taacgtgttg atcgaggata gtttatctcc gggcttcaat
     1381 tcgttcatcg atatagccct ctggatatag tcggctaatt gctttacttt cgtattcttg
     1441 ttgttgaatt ccaattccat gataaactag tgtgctgttc tgttctgatc acaaaggtat
     1501 tttttattta ttgattttcg ctttaagccc atatgtttta taacataaat agttaatcac
     1561 ctgttttcaa acgaaagatt cagctgcaat aaaaaaaccg gcaaggctct tcacagagtc
     1621 ttgccggcca tggaaaatca aaaagtcata taagaaatag aactaattaa gttatggtta
     1681 tttaatagta ttcgttcgat gcaacaaccg gtatgggtgt ttcagcgatt ttgttttgca
     1741 acgcagcctg gttgtacctg taaaccacgt tgaatatttt ttctccatcc tcgtctttcg
     1801 cttttaccgt tagttgaacg gaatcggtgt ccgctcgttg gagcggtttc agatcgaaac
     1861 agataaaccc ctcgaaaaac ttcgggcttg agctgccgta agcattgtgc cggaactcca
     1921 gttcaagggt gtcgggggct ttgtccggat gcaatgaatc aatcaccagg ttgatggcgt
     1981 gaggcctgac tcctccgtaa ttgaacataa aatcgatatt caggtagtcg tttcctaccc
     2041 atgcttcgtt tatttttacc ggatcatgcc ctatgctgtc ggcgttttgc gacgtttgtt
     2101 cgatgacctt tttagtaaga atattccaga tgtcgtttac tttcacgtaa tgatcatatc
     2161 caccctgttc atccgataag gtggtgtaat tcacaaatac cctctggtag ttccccactg
     2221 gcaggtagtg cacatccgtt gcagccggcc agagttgctt gccgttgtcg agcattagca
     2281 tgtatccgtt ctctcccatg ggaatgaccg tagcaatgtc aatatgaaat tcgcccagcg
     2341 aatgttcgtc tttcttgttg cacgatgcaa ctgtaagtaa aacgataaaa aaaacaaatc
     2401 cccacgcggt atctttcata tatttctttg cttctatata cttagatgca aaaaagaaaa
     2461 aaacgctgca tgaaaacctt ggattttttt gtttttaact ctatttaact tttggataga
     2521 gtttctcaat aagatcggtg cgattgagcc gtttcaactc tgctattaat ttcttccggt
     2581 tgttcttgtc gtaccagaaa aaaaattgtc gttgactttc tttttgtgaa ggggattttg
     2641 cggtgtaaac cttttcgagc gaataagggt ggtagcctgt gtaatagatc tctgtagcca
     2701 gtgtcatggg cgagggtgtg aagtcctgta tttgctccag tttgaaattc aggtttttag
     2761 taatagcggc aagttctgcc atatcttctt ctgtgcaacc cgggtgtgag gaaatgaaat
     2821 aagggatcaa ttgctgattc aggttctgct tgttgttttc agcatcgaaa atctttttga
     2881 attgataaaa caggccgaac gaaggcttac gcatcacgtt gagcgtccgg tctgccgtat
     2941 gttcgggagc gactttgagc ctgccggata cgtggtttac aatcaactct tttgtgtatt
     3001 cttcggcggc tttgttggca ctttcgtctt ttcccctatg cagcagcatg tcgtaacgga
     3061 tcccgcttcc gataaacgat tttttcacca tcggcaggtt gtccacagag cgatagatgt
     3121 cgagcagtgg agcgtgattg gtattgagat ttgggcaaat accgggatgg atgcacgacg
     3181 ggcgtctgca cttttcacaa accgacaggt ctttgccctg cattttgtac atgtttgccg
     3241 acggccctcc caaatcgctt atgtatcctt tgaatccggg catttcggtg attttcccga
     3301 cttcccggag aatcgattct tttgatcgcg atgcgataaa ctttccctgg tgtgccgaga
     3361 tggtacaaaa agcacatcct ccaaaacaac cccggtgcag gtttactgaa aacttgatca
     3421 tttcgtaagc cgcaatgtgt ttgtttttgt acttgggatg aggcaagcgg gtgtatggta
     3481 aatcaaaaga tgcatcaatt tcctcttccg tcatcggggg aaaaggtgga ttcacaacca
     3541 gcgtcttatt gtccacatcc tgtaagatac gcgatgcgtg caacgcgttg gactgctctt
     3601 ccacaatacg aaagtttttt gcctgtttca gcctgtcttc caggcattct tcatgagaga
     3661 ataaccggat ctcgtcttta aatgaatttt caggaacatt gtcttttccc gcaaggaaag
     3721 ctgtctgggg cacatggcac atttcggcaa tcgttttccc gttttgcatt tcccggatca
     3781 cgcgggtaat cggcagttcg cccatgccgt aaaccagcaa gtcggcgttg gtgcctgcca
     3841 gtatcgtttt atgcagagaa tcactccagt aatcgtaatg cgaaacccgg cggagggaag
     3901 cttccacacc tccgataatc agcggaatgt cgggatagag ttctttcagg atgttggaat
     3961 aaaccgttac cgcccggtcg gggcgcatat ccgacctccc gtcgggtgtg taggcgtcct
     4021 gcgatcgtaa gcgtttattg gcggtgtatc ggttgatcat cgagtccata acaccggcgg
     4081 ttacggcaaa aaacattcgg ggaagcccca gttttttaaa atcccgcaaa tcgtccctcc
     4141 agtttggttg aggaacaatg gctacgcgca accccaacct ttccagcagt cgtccaatca
     4201 ccgcagtccc aaacgaagga tgatcgatgt aggcatctcc cgaaaagagg atcacgtccg
     4261 cctggtccca cccgagaaga tccatctctt ttttggtggt gggtaggaag tgtgccgatg
     4321 gatagggttg gctttccatc cgttaaaaga gtttatgttt cctatgtctc tcttctccca
     4381 tcttctcgct cagtacgatc gccctgtcca ccgccaggtg gatgtttgaa cagatgttct
     4441 cttttccgat aatatccggt attttggagt tttcgacttt tgccctgaca ttgccattca
     4501 ctcccgaaag aataacctga attttttctc tccgggagtt tttgcagaga ttttggaggt
     4561 tgttcagccc cgtagcatcg ataaacggca ccttgcgcat ccggataatc cggatctgtg
     4621 ccctttctcc gatttcgcgc ataacatcgt caaacttatt ggctacccca aagaaaaaag
     4681 gcccgtcaat ttcatacact tccacgcctt taggcaggtg caagacttcg tcctgcaagt
     4741 tcccggaatg agactcaatc tcttttgaga tgtcaatctt tccggttgtt accgacactt
     4801 gagtgctttc gttcatacgc ttcaaaaaca agaagatggc aagcaatagc cctacttcaa
     4861 tggcgatggt caggtcgaat actaccgtta gaataaacgt cgtcagtaaa acggccagcg
     4921 ttcctccaga ctgtttgaca agtgagcgga acgtgcgcca ttcgctcatg ttgtatgcca
     4981 ccacaaccag cactccggca aggcaggcca taggaatatg tttggtaaga tcacccaaaa
     5041 acaatagaat cagcaacaga accactgcat gtataacccc tgccaccgga gaacgcccac
     5101 cgttgttgat gttggtcatt gtccgggcaa tggcgccggt agccgggatc ccgccaaaga
     5161 acggagtcac gatgttggct actccctgtg caaccagttc catgttcgag ttgtgccgtt
     5221 taccgatcac gccatctgca acggttgcag acaacaggga ttcaatagcc cccagtatgg
     5281 caatggtaaa tgctgaagga aacagcatgc ggatgctttc gagattgaac ccgataggat
     5341 ttgcctgagg cagttggctg tttatctgaa acctgtcgcc gatagtttca attccggtaa
     5401 ttccaaaata gtgcttcatc acgaacacga taacagtcat caaaacaatc gctatcagcg
     5461 atccgggtat tttcctggaa atttttggag tgagaaagat gatggtcaca ctcaacacgc
     5521 caatgcctgt cgcccaccaa ttgatggttt ccaaatgttg aaaatatact tcccatttgg
     5581 taaagaaatc ggaaggaacc ttggctatac tcaatccaaa caagtctttt atctgcgtgg
     5641 agaaaatggt aagggcaata ccactggtga acccaacaac aatggggtag ggaatgaact
     5701 tgatgacagt cccgagcttg agaacaccca ttaaaaccag catggctccg gcaagaacag
     5761 tagctattgc cagcccggtc acgccaaact gttccacaat tccgtaaaca ataacaataa
     5821 atgctcctgt cgggccgcca atttgtacat ggcttccgcc gaggaacgaa ataataaatc
     5881 cggcaatgat tgccgttacg ataccttttt ccggtgtgac accggaggag attccaaaag
     5941 caatggccag cggcaatgcc acgattccta cgataaggcc tgccattaaa tcggaaataa
     6001 atttctccct gttgtagttt tttaacgata agaaaaggat gggtttaaat tctttcagaa
     6061 agattttttg catctgtata ttcaataatt ggctgcaaaa ttaaccattt aaagaataaa
     6121 aatgaaattg tacgtgtaaa atttagaaag caccctgttc cacaagcacg caggcgcgtt
     6181 ccgttaagcg atcttcggca taggggttgt acctcttttt gaggctgttg ccaaacatgc
     6241 ttgcaaacgc attgtagaaa aaagccttgg cacttcccat gtaggcgtca ataatgtggt
     6301 acgacgatga gttggtctcc cgatcaacca gcttcatcaa a
//
LOCUS       DAOSDR010000182         6335 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrimonas sp. isolate AMR_MDS_1826
            NODE_4439_length_6335_cov_3.347611, whole genome shotgun sequence.
ACCESSION   DAOSDR010000182 DAOSDR010000000
VERSION     DAOSDR010000182.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863355
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrimonas sp. (wastewater metagenome)
  ORGANISM  Petrimonas sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Dysgonomonadaceae; Petrimonas.
REFERENCE   1  (bases 1 to 6335)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6335)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:10:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,720
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 35
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6335
                     /organism="Petrimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4439_length_6335_cov_3.347611"
                     /isolate="AMR_MDS_1826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2023866"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2861)
                     /locus_tag="PLI30_08100"
     CDS             complement(<1..2861)
                     /locus_tag="PLI30_08100"
                     /inference="COORDINATES: protein motif:HMM:TIGR04056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0071702 - organic substance transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SusC/RagA family TonB-linked outer membrane
                     protein"
                     /protein_id="HOI79552.1"
                     /translation="MKKNREVIGRTKFPEIEKLFRIMKLTSFFLILSIVSVWATETYS
                     QTKTLTLNVERTTIKEVLSKIEEQSEFSFMYSGKYIDVNREVTLHVENQKVESILEAL
                     FAGTNISYTIKDRFIVLSTPGMRGTLAVGQQPKNISGKVTDISGLPLPGVTVLVKGTA
                     NGTITDVDGDYSLDNVTEDATLIFSFVGMKMKEVEIGNKSIINVILEEESVAVEEVTV
                     VGYGIQKRAKVTGAISSVKMDEVVGSRSNTNVSQLLQGNIPGLQITYSSGEPGTSTKF
                     NIRGITSIGLGSNSSPLILLDNVPIASLDLVNPNDIETVTVLKDAGSAAVYGARAAWG
                     VVLVTSKNSAKKQKTKFEYSNNIVLSQPIEIPRKAAPSQVIQSFKDMNFVPGTGQNID
                     TWLSLLNIYNQDPTQYPLGYTFQNGVIYPLKETDVWKDMMSNHSFQQTHNLTVSGSSD
                     KSSYRISGGSTNEDGILVTNKDSYSRINLNGFISTDVAPWLTAQLTTHYNNSNKKSPY
                     TGYSFGIFGSAAVQPSYQILGDSLINGERVPYATPRHLIEGSQPNNNRIDNMRLSGKV
                     ILKPLSSLSIVGEYTYEKTNTVFTQYDKAFNIITLSSFIPTPTVTQDQAKYYKYNDFT
                     DYHALNIYATYERKFQEDHLITLMCGLNREDSYSELLNAKMSNMINPNLPYLDGGTGT
                     IHPDGNGFDGFSEYAVAGYFYRFNYTYKGKYLLETTGRYDGSSKFPTGNRWGFFPSFS
                     LGWRVTDESFMKSLNPVLSDLKLRASYGNIGNQSIGNYLYYAGMTTRPTSNWTDASGV
                     RYLTLNPPSLISSNFTWEKVTTINLGLDFGLLNNKLSGMFDLYQRDTKEMLIPGAQLP
                     SVLGASSPLQNSANLTSTGWELQVTWKDRIGKFNYNLGFNLFDNQGKITKYDINSTGL
                     LSQHYVGEKIGEIWGYVSDGFYTVDDFVSGTLNGTLTGG"
     gene            complement(3011..4084)
                     /locus_tag="PLI30_08105"
     CDS             complement(3011..4084)
                     /locus_tag="PLI30_08105"
                     /inference="COORDINATES: protein motif:HMM:NF016648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4974 domain-containing protein"
                     /protein_id="HOI79553.1"
                     /translation="MKKETIIKFLNNHSTEAELEEVVRWAKEEAFSKESMEWGLRDWN
                     LYQASEEGEDDEKFSLLFDKIQLQINAKGNDLQQKGTRRALLPLLVSWSTRAAAILLI
                     PVLVLLFYTLSEKRAVSIRYANQRIDSLEIIAPIGSRSVLQLPDGSTVHLNYGSKIKY
                     PQIFSGNTREILLEGEGYFDVVTDPDKPFIVKAGGLSVKALGTAFNVLAYPDREIIET
                     TLVSGKVVLEQNRTNGKVKPIGVLLPGMHVAFNSETGSISKTQGNVEKYIGWKDGKLI
                     FEDATIAQVADRLNRMFNVDIEIADDNDVKDFIYTVTFVNEPLFQILDLMTIATPIKY
                     KALPRMKMPDGTFSKQKIIIEKR"
     gene            complement(4212..4790)
                     /locus_tag="PLI30_08110"
     CDS             complement(4212..4790)
                     /locus_tag="PLI30_08110"
                     /inference="COORDINATES: protein motif:HMM:NF019265.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma-70 factor"
                     /protein_id="HOI79554.1"
                     /translation="MKSNSELLLLVKEGDRVAFYNIYERYCRRLYGFVLRYIKLEEDA
                     EEIVQEVFVKIWENRHKIDAYSSFESFLFTIAYNTTMTLFRKRVNEKKYLEHLKSLQT
                     FEQAPDLLDEIHFNELSRRVEMLLNELTPRQKEIFQLSREKGLTHDEIAQELNISVNT
                     VKKHMGNTLAFLKTHIDTSLMVNLLFVHLFLS"
     gene            complement(4911..4983)
                     /locus_tag="PLI30_08115"
     tRNA            complement(4911..4983)
                     /locus_tag="PLI30_08115"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4948..4950),aa:Lys,seq:ttt)
     gene            5231..6028
                     /locus_tag="PLI30_08120"
     CDS             5231..6028
                     /locus_tag="PLI30_08120"
                     /inference="COORDINATES: protein motif:HMM:NF019590.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NIPSNAP family protein"
                     /protein_id="HOI79555.1"
                     /translation="MKIEHQSAAGRTTALLFLLLFLAVAPLAGNARDYYAIKIYRFKE
                     QSQVERVEKFLKEAYLPALHRAGISTVGVFKPIEGDTVAGLSVFVWIPLKSLAQFEGL
                     AQKLEKDLQFRAAGATYLEAAHNQPPYSRIETILLKSFQDMPQFKIPHHQTPPAERIY
                     ELRSYEGPTEKLFQKKVEMFNAGGEIKLFEKLDFNAVFYAEVIAGSTMPNLMYLTTFA
                     DPKTHADRWNTFRNHPEWKELSAMEKYKNTVSRSVKYLLHPTDYSDF"
     gene            6042..>6335
                     /locus_tag="PLI30_08125"
     CDS             6042..>6335
                     /locus_tag="PLI30_08125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008760975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="9-O-acetylesterase"
                     /protein_id="HOI79556.1"
                     /translation="MNKKIFTLAGVLTLLLLIPAILQAEVKLPVIFGDHMVLQQQTDA
                     AIWGKATPGKTVRVTTSWNQKSYATQADATGAWKLKVKTPAAGGPYSFTITDGK"
BASE COUNT         1738 a         1457 c         1233 g         1907 t
ORIGIN      
        1 ttccctcccg taagtgttcc attcagtgtt ccactcacaa agtcatcaac tgtatagaaa
       61 ccatcagata cataacccca gatctccccg atcttttcac ctacatagtg ttgagataaa
      121 agtccagtag agttaatgtc gtatttagta attttgcctt gattgtcaaa aaggttaaag
      181 ccaagattat aattaaactt gcctatacgg tctttccagg tcacctgtag ttcccaaccc
      241 gttgaagtca aattggccga attttgcagc ggagaacttg cgcctaatac ggaaggcaat
      301 tgagcgcccg ggatcaacat ttctttcgta tctctttggt aaagatcaaa cattccggat
      361 aatttattat ttaggaggcc aaaatctaaa ccaaggttga tcgttgttac tttttcccat
      421 gtgaaattgg aacttatcag tgagggtgga ttaagtgtca gatatcttac accagaagcg
      481 tctgtccagt ttgaggttgg gcgggttgtc ataccggcat aataaagata atttccaata
      541 gattgattac caatatttcc atagctggct ctgagtttaa gatcagaaag aacagggttt
      601 agactcttca tgaaagactc atcagtgacc ctccatccta aagaaaagga tggaaagaaa
      661 ccccaacggt tacctgttgg aaattttgaa gatccatcat atctgccggt agtttctaat
      721 aggtatttcc ccttataggt gtaattaaac cgatagaaat atccggcaac agcatattcg
      781 gagaaaccgt cgaatccatt tccatcagga tgaatggttc ctgttccgcc atccagataa
      841 ggaagatttg gattaatcat gttggacatt tttgcgttaa gcaattccga ataggagtct
      901 tcacgattca acccgcacat gagtgtgata agatgatctt cctgaaattt tctttcataa
      961 gttgcataaa tattcagggc atgataatcg gtgaaatcat tgtatttgta atattttgcc
     1021 tgatcttggg taacagtagg cgttggaata aaactactta atgttattat attaaaagct
     1081 ttatcatatt gagtgaaaac agtatttgtt ttttcataag tgtattcacc aactatggag
     1141 aggctggaaa gtggtttaag tattacttta ccagataatc gcatattatc aattcggttg
     1201 ttatttggtt gggaaccttc aattagatgt cgtggggtag cgtaaggcac acgttctccg
     1261 ttaataagtg aatcacctag aatttgatag gatggttgca ctgcggctga cccaaatatt
     1321 ccaaatgaat accctgtata gggtgatttt ttgtttgaat tattgtagtg cgttgtaagc
     1381 tgtgcagtta accaaggagc tacgtctgta ctaataaaac cgtttagatt tatacggctg
     1441 tatgaatcct tatttgtgac caatataccg tcttcatttg tagatccgcc agagattctg
     1501 taggatgact tatcactact tccactaact gtgagattat gggtttgttg gaatgaatga
     1561 ttggacatca tgtctttcca aacatctgtt tcctttaggg gatatattac gccattttgg
     1621 aaggtatatc ccaatggata ttgagtagga tcctggttgt atatattcaa taaactcaac
     1681 caagtatcaa tattctgacc agtgccaggg acaaaattca tatctttaaa tgattgtatc
     1741 acttggctgg gagcagcttt acgcggaatc tcaatgggtt ggctaagtac gatattattt
     1801 gagtattcaa actttgtctt ttgttttttc gcactgtttt tactcgtaac caagactacc
     1861 ccccatgctg ccctggcccc gtaaacagca gctgaaccag catcctttaa tacagttaca
     1921 gtttcgatat catttggatt tactaaatcc agcgatgcga taggcacatt gtccaacaaa
     1981 atcaacggag aactgttgga tcctaaaccg atactcgtta tccctctgat attgaatttc
     2041 gtggatgtcc ccggttctcc ggatgaatag gttatctgaa gtccgggaat attcccttgt
     2101 aaaagttgtg atacattggt atttgaacgg ctaccaacaa cctcatccat cttgacagag
     2161 ctgatcgcac cggtgacttt agcccgtttt tgtatcccat acccaactac cgtgacctct
     2221 tcaacagcta ccgattcctc ttccagtatc acattgataa tagatttgtt tccaatttca
     2281 acttccttca tcttcatccc cacaaacgaa aagatcaaag ttgcatcttc cgtaacattg
     2341 tccagtgaat aatccccgtc tacatcggta atcgtaccgt tggctgttcc ttttaccaac
     2401 acggttactc ccggcaaagg caatccggag atatcggtta ctttccccga aatgtttttt
     2461 ggttgctgcc caacagccag ggtacctctc attccaggag tagacagtac aataaacctg
     2521 tctttgatag tataactgat attggtcccg gcaaacaatg cctctaatat tgactcaacc
     2581 ttctgattct cgacgtgcaa cgtgacctct ctgttcacat caatatattt accactgtac
     2641 ataaacgaga actcgctttg ctcttcgatt ttggacagta cttctttaat tgtggtcctc
     2701 tccacattaa gcgtcaatgt ttttgtttgg gaataggttt cagttgccca aacactgact
     2761 atcgaaagta tgagaaagaa gctagttagt ttcataatcc tgaacaattt ttctatttcg
     2821 ggaaattttg tcctcccgat cacttctcta ttttttttca taattttgtg ttgattagat
     2881 ttatactacc ctggatgcag ggttaaatta tttacaatca ccagggagat gttggcgcat
     2941 ttccctgttt tgtttagctc gttttatctc ttcataggct gaatagtttt tatgggttag
     3001 taacggctca ttatcttttt tcaattatta ttttttgttt cgagaaggta ccatccggca
     3061 ttttcatcct cgggagtgcc ttgtatttta ttggagttgc aatggtcatc aggtcgagta
     3121 tctgaaaaag cggctcattg acaaacgtaa ccgtgtagat aaaatcttta acgtcattgt
     3181 catccgcaat ctcaatgtca acattaaaca ttcggttcag ccgatcggca acctgggcaa
     3241 tggttgcatc ttcaaaaatt agttttccat ccttccaacc aatatatttc tccacattcc
     3301 cctgggtttt tgaaatgctc ccggtctcag aattgaacgc cacatgcatc ccgggcaaca
     3361 ggactccaat gggtttaacc tttccattcg ttctgttttg ctccaaaaca acctttccgc
     3421 tcaccagcgt tgtttcaata atttcccggt cgggataagc cagcacatta aaagcagtcc
     3481 ccagagcttt tacgctcaat cctcctgcct ttacaataaa aggtttatcc ggatcggtaa
     3541 ccacatcgaa atacccctcg ccttccagca atatttctct ggtatttccc gaaaaaattt
     3601 gcggatattt aatcttgctg ccgtagttta aatgcacggt ggacccgtcg ggtaactgga
     3661 gcacgcttct tgacccaatg ggcgctataa tctccaacga atcaatccgc tggtttgcat
     3721 acctgattga cactgccctt ttttctgaaa gtgtataaaa caggagaaca agaaccggta
     3781 tcaataaaat ggcagccgcc ctggttgacc agctcaccaa caagggcagg agtgctctcc
     3841 gagttccctt ctgttgaaga tcgttacctt tcgcgtttat ttggagttgg atcttgtcaa
     3901 acagcagact gaatttttca tcatcttccc cctcctcgct cgcctgatac agattccagt
     3961 cacggagccc ccactccata ctttctttac tgaaagcttc ctcttttgcc catctgacaa
     4021 cctcttccag ctcagcctcg gtagagtggt tattcaaaaa ttttataatc gtttcctttt
     4081 tcattttaat cctctggctg catttcctca acagaaggta caattaagcc tgacaaatgc
     4141 tcctgtgttt tgtttcgaaa agtaatacgc tcaaacagca ataagggagt atatgaaata
     4201 tgaaaaaaat ttcatgaaag gaacagatgc acaaacaaca aattgaccat cagactggta
     4261 tcaatatgag tttttaaaaa agcaagggta ttgcccatgt gttttttgac ggtattaacc
     4321 gaaatattga gctcttgggc tatctcgtca tgggtcagcc ctttttctcg gctgagttgg
     4381 aatatctctt tttggcgcgg agtcagctcg tttaacagca tttccaccct cctgctcaac
     4441 tcgttgaaat gaatttcatc caaaaggtcg ggagcttgct caaatgtctg caaggatttt
     4501 agatgttcca ggtatttttt ttcatttacc ctcttgcgaa aaagggtcat cgtagtatta
     4561 taggcaatcg taaacaaaaa ggactcgaaa gaggagtaag catcgatctt gtgccggttt
     4621 tcccagattt taacaaacac ttcctgaaca atctcttcgg catcttcttc cagcttgata
     4681 taccgcaata cgaatccata taaccttctg caatatcttt catagatatt atagaaagct
     4741 accctgtctc cctctttaac cagaagcaaa agctcgctat ttgacttcaa acatttcaag
     4801 acaacgaaaa atgagcttca aagatatact attttctcag gcagaaattc caggcagctc
     4861 aatagaaaaa gccctgcaga tttaaatctg cagggcttac tgctaagcgg tgactcagct
     4921 gggattcgaa cccaggaccc catccttaaa agggatgtgc tctacctgct gagctactga
     4981 gtcatccttt ttgatgttcc tttgtttctc aaaggcgggt gcaaaagtag tattattttt
     5041 tttactcaca aatcttttta attttttatt gcaggaaaat ccgccccctt tggcagcccg
     5101 gacattctcc acaaatatta tcttctcaca accagaatag tagtaaaaaa taccgcccac
     5161 gctgcccaaa tgttattttt gattttttta tattgcgctc ttaatttttt taaaccaaaa
     5221 gacttttttt atgaaaatag agcaccaatc cgctgcaggg cgaaccaccg cccttctttt
     5281 tttactgctg tttttagccg tggccccttt ggccggcaat gcccgcgact attatgcgat
     5341 caaaatctac cggttcaagg agcaatcaca ggttgaacgg gtggaaaagt tcctgaaaga
     5401 agcttatctg cccgccctgc accgggccgg catcagcacg gtgggcgttt ttaaacccat
     5461 cgagggagac acggtcgccg gtttatcggt tttcgtctgg attccgctca aatcgcttgc
     5521 ccagttcgag ggtttagcgc agaagcttga aaaagacctg caattccggg ctgccggcgc
     5581 cacttacctc gaagcggccc ataaccagcc accctacagc cggatcgaaa ccatcctgct
     5641 gaaatctttt caggatatgc cgcagtttaa aattccccac caccagactc cgccggcgga
     5701 acgtatttac gaattgcgca gctacgaagg tcccaccgaa aaattatttc agaaaaaagt
     5761 ggagatgttc aatgccggcg gggagatcaa actgttcgaa aagctcgatt tcaatgccgt
     5821 attttatgcc gaagtgattg ccggcagcac catgcccaac ctgatgtacc tgacaacttt
     5881 tgcagatccg aaaacacacg ccgaccggtg gaacactttc cgcaaccacc ccgaatggaa
     5941 agagctttcg gccatggaaa aatataaaaa cacggtttca cgctcggtga aatacctttt
     6001 acacccaacc gattattctg acttttaata tttaccaaac catgaacaaa aaaatcttta
     6061 ccctggcagg cgtattgacc cttctcctgc tcatcccggc tatcttgcag gccgaagtaa
     6121 aacttccggt tatttttggc gaccacatgg tgctgcagca gcagaccgat gcagccatct
     6181 ggggaaaagc gacgcccgga aaaacggtgc gtgtcaccac ttcgtggaac cagaaaagct
     6241 acgccaccca ggcggatgcc accggcgcct ggaagctgaa agtgaaaacc ccggccgcag
     6301 gcggtcctta cagcttcacc atcaccgacg gaaag
//
LOCUS       DAOSDR010000183         6207 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrimonas sp. isolate AMR_MDS_1826
            NODE_4539_length_6207_cov_4.023082, whole genome shotgun sequence.
ACCESSION   DAOSDR010000183 DAOSDR010000000
VERSION     DAOSDR010000183.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863355
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrimonas sp. (wastewater metagenome)
  ORGANISM  Petrimonas sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Dysgonomonadaceae; Petrimonas.
REFERENCE   1  (bases 1 to 6207)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6207)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:10:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,720
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 35
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6207
                     /organism="Petrimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4539_length_6207_cov_4.023082"
                     /isolate="AMR_MDS_1826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2023866"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1302)
                     /locus_tag="PLI30_08130"
     CDS             complement(<1..1302)
                     /locus_tag="PLI30_08130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006744860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HOI79557.1"
                     /translation="MKKLIYILMVILLTVGVAGCSDDFLSASSTEKQEAGAPAYEGAI
                     LANLASAYQILLFDSYANQNYNSIPLMSDLRSDDIFKGGGDAGDQRQLYLLSLFTSTP
                     QELPEGLWAILYSGIARANNAISACENAVEVPEEKVNQYKAEAHFLRAYYTHWLWKFW
                     GNVPYFEEPLTTPPYMTRQYTADEIYAEIMQDIDVATTDGWLPMSTTGSNTGRINKAT
                     ALMLKARVVMYQKDQSRYAEVTADMAEIINSNEYDLFGDFAAMWLDENEFCKESIFEV
                     NHLPEGKIWANGWQGYGTNLPAFISPNGLNTGNKTGDFKGGWGFGPVRQSTWDIYEGG
                     DTRREGSINKWEPEQYTARFQDTGLFMAKYAARVGYNPQGDVDLNYCNNLRVFRYAEA
                     LLTYAEMVVMHGQSPVGGITAQACLDEVRLRAFGKASSIPAT"
     gene            complement(1321..4782)
                     /locus_tag="PLI30_08135"
     CDS             complement(1321..4782)
                     /locus_tag="PLI30_08135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019540036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOI79558.1"
                     /translation="MKRRLMMLLSFFVLGTAMLFAQKVNLNYNKATLRTVLESITEQI
                     GHTLAFSKEAVDLSDEVTIRVTDGEAAQVLNQLLTPRQIGYEIRDNKIYIFDQPVPVN
                     AVTRQTPLQEVRLTGTVTDVNGEPIIGANIVVPGTTIGTVTNVDGTYSMTLPRGSVLR
                     FSYIGYTGQEFTIANQTVLNVQLQEDTEVLDELVVVGYGVQRKSVVTAAISKVSAEEL
                     NVTRPARVEDALKGKVSGVQITQSSGQPGSDSKVRIRGIGTINNSEPLYIVDGMEVGG
                     GINYLNPVDIASVEILKDAASAAIYGSRAANGVILVTTKKGDKGKATVNYDVSYGWQN
                     PWKKKEILDAREYMVIMNEAQINDGNLPRYNNEQVMGAGKGTDWQDETFYYNAPVQNH
                     QVSVSGGTDKGNYFLSFGYFDQAGIVGGNYDKSNFERYSLRSNSIYNIFETKERSFLN
                     NVQLGVNVGYSRNKSTGIETNSEYGSILGSALTFSPLVPVYADEETGKAILAQYPHAV
                     KNDDRVFSIPPAGFQEIANPVGMLNQPSAGLNNADKFVGSFWGELTILPELKFRSSYG
                     VDLAFWGYDSYTFPYFLATQGKDVQFSTVQSEMNRGYTWQLENYFSYNKSFEEIHNLS
                     FVLGQSASKYTYRNLGGNDRDLLENDPLKANINYAIADRKEERAWGGTGGYNFTARAS
                     YFGRIDYNYDEKYMLQATVRRDGSSNFGPGHKWGVFPSFSAGWNVTNEAFMEGRPQWF
                     DYMKLRASWGKNGNDRIDQFLYTSLMDGGQNYYFGGGYDVNQGDPAKVGEITGQMQYG
                     SAPGYIPNPNVKWEESSQTNLGIDTRFLQSRMTFSFDYFKKKTIDMLMKQPIPSYVGI
                     GAPIANVGDMENWGLEFELGWKQSVGEFNYAVSANASYLKNKLINLGNETGEQIYENA
                     GASGVGSYVKGMNGDVFPYFYGYKTDGLFQNQTEVDAYVNADGEKYQSAALPGDVRFV
                     DLNGDGVISDADKTKIGKGMPDWTYGLTLSADWKGVDLNLFFQGTIGNDVFDFSQRGD
                     IPAMNRPAWILDRWHGEGTSSHIPRMTSANPNGNWRSSDLYIKDGSYMRLKSAQLGYT
                     LPVDLTGKIAVQRLRIYVSADNLLTFTSYDGFDPEIASGGYTTIGIDRGIYPQSRTIS
                     VGANITF"
     gene            complement(5102..5962)
                     /locus_tag="PLI30_08140"
     CDS             complement(5102..5962)
                     /locus_tag="PLI30_08140"
                     /inference="COORDINATES: protein motif:HMM:NF016648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR domain-containing protein"
                     /protein_id="HOI79559.1"
                     /translation="MLDRDAYLMDADADSGKSFTPLQSDLLFRRIDKDIRIKRLRTVT
                     RNVAAVLVPILFLLGLGYFIHSQTSFFQGTTYAELYIPKGEDARIFFQDGTEVFLNAD
                     TKIRYPEHFGFRHREVYLDGEAYFNVAPDRKRPFVVHAQNTSVEVLGTAFNVNAYSES
                     ETIQVVLDEGKTSFQVQQSKYPMSPGQQIEYNKTTGKTSLYNLSKPSNVSLWRKNVLY
                     FYDTPLAEVIKTVERKYNVHFHVQSPEALNFSYTLTTKQLHLEDLLNELQKIAPVKFQ
                     YKGDNVFVSI"
BASE COUNT         1509 a         1578 c         1409 g         1711 t
ORIGIN      
        1 ggtggcggga atggaactcg cttttccgaa tgcacgcagg cgaacttcat ccaggcatgc
       61 ctgggctgta atgccgccaa ccgggctttg tccgtgcatc actaccatct cggcgtaagt
      121 aagcaatgcc tctgcatagc ggaaaacccg caagttgtta caataattca gatcgacatc
      181 tccctggggg ttgtaaccta cccttgcggc gtacttggcc atgaacaggc ctgtatcctg
      241 aaagcgggcg gtatactgtt ccggttccca tttgttgatg gaaccttccc ggcgagtgtc
      301 tcccccttca tagatgtccc aagtggactg acgaaccggc ccgaatcccc agcctccttt
      361 gaaatcacca gttttattgc cggtattcaa tccgttggga gaaataaatg cggggaggtt
      421 ggttccgtaa ccctgccagc cattggccca aattttccct tcgggcaagt ggtttacttc
      481 gaagattgat tctttacaga actcattctc atccagccac atcgcagcaa aatcgccaaa
      541 taggtcatat tcattgctat tgatgatttc cgccatatca gctgttacct cagcgtagcg
      601 cgactgatct ttttgataca tgaccacacg cgctttcaac atcaatgccg tagccttgtt
      661 tattctaccg gtattcgacc ctgttgtact cattggcaac cagccgtccg ttgtagccac
      721 atcaatatcc tgcatgatct cggcgtagat ctcatcagct gtgtattgcc tggtcatata
      781 gggcggcgtt gtcagcggct cttcaaaata gggcacattg ccccagaact tccaaagcca
      841 gtgggtataa taggcacgca gaaagtgtgc ttctgctttg tactgattta ccttctcttc
      901 cggaacctct accgcatttt cacaggcgga gatcgcattg ttggcacggg caattcctga
      961 atagaggata gcccacaacc cttcgggaag ttcctgcggg gtggaggtaa aaagcgacaa
     1021 caggtacagt tgacgctggt cgcccgcatc accgccccct ttgaagatat cgtccgaacg
     1081 aaggtcggac atcaaaggga tactgttgta gttctgattg gcatagctat caaacaacaa
     1141 gatctggtaa gccgatgcga ggttcgctaa aattgctccc tcgtaagcag gagcacccgc
     1201 ctcttgcttc tctgtggatg aagcactgag aaaatcatcg ctgcatccgg ccactcctac
     1261 tgtcaggagt atcaccataa gtatatatat taatttcttc atttctgtac aatgttaaga
     1321 ttagaatgtg atgttggcac ccactgaaat ggttcttgac tgtggataga taccccggtc
     1381 gatcccaatg gtggtatacc ctccggaggc gatctcagga tcaaacccgt cgtaggaggt
     1441 aaatgtaagc aggttatcgg ccgagacata gattctcagg cgctgcaccg ctatttttcc
     1501 ggtcagatcc accggtaagg tgtatcccag ctgggccgat ttcaggcgca tataggatcc
     1561 gtccttgatg tagaggtcgg aggatctcca gttcccgtta gggttggccg aggtcatgcg
     1621 tgggatgtgg ctcgatgtgc cttccccatg ccagcgatcg aggatccagg cgggacggtt
     1681 catggcaggt atatcgccac gttgtgaaaa atcgaacaca tcattcccga tggtaccctg
     1741 aaagaagagg ttcaggtcga ctcctttcca gtcggcagag agtgtaagcc cgtaagtcca
     1801 gtctggcatc ccctttccta tcttggtttt gtcggcatcg ctaatcacac cgtcgccgtt
     1861 caggtccaca aagcgtacgt cacccggcag ggctgcactc tggtattttt ctccatcggc
     1921 attcacgtaa gcatccactt ccgtctggtt ctggaagagt ccatcggttt tataaccgta
     1981 aaaataggga aatacatccc cgttcatacc ttttacatag cttcctacgc cagaggcacc
     2041 tgcattctcg tagatctgtt ctccggtttc gttccccagg ttgataagtt tgttcttcag
     2101 gtaagaggcg ttggccgata cggcatagtt aaattcccct accgactgct tccagcccag
     2161 ttcaaattcc aacccccagt tttccatatc gcccacgttg gcaatagggg cgccaatacc
     2221 cacataggaa ggtataggct gcttcatcag catatcgatg gtttttttct taaagtagtc
     2281 gaaagagaag gtcatccggc tttgcagaaa acgcgtatca atcccgaggt tggtctgtga
     2341 cgactcttcc catttcacat taggattagg aatgtaaccc ggagcacttc cgtattgcat
     2401 ctgcccggta atctcaccca ccttggccgg atctccctgg ttcacatcgt acccgcctcc
     2461 aaagtaatag ttttgtcctc cgtccatcaa cgaagtataa aggaactggt cgatacggtc
     2521 gttaccgttc tttccccagc tggcacgcag tttcatatag tcaaaccatt gcggccgccc
     2581 ctccataaat gcttcatttg tcacgttcca tccagcagag aaggagggga agacacccca
     2641 tttgtggccg gggccgaaat tggaagatcc atcgcggcga accgtggctt gcagcatgta
     2701 cttctcgtca tagttgtaat cgatcctgcc gaagtaagaa gcgcgggccg tgaagttata
     2761 tcctccggta ccgccccagg cacgttcttc ctttctatcg gcgatagcat aatttatgtt
     2821 tgccttcagc gggtcgttct ccagcaggtc gcggtcgttg ccgcccaggt ttcggtaggt
     2881 atattttgat gcggattgtc cgagcacaaa agagaggttg tgtatttcct caaacgattt
     2941 gttgtaggag aagtaatttt caagctgcca ggtgtagccg cggttcatct cgctttggac
     3001 cgtactgaac tgcacgtcct taccctgtgt cgccaggaag taagggaatg tatatgaatc
     3061 atatccccag aatgccaggt ctacaccgta actgcttctg aacttcaatt ccggcagtat
     3121 ggtcaactcg ccccaaaatg agccgacaaa tttatcggcg ttgttcagtc cagccgaggg
     3181 ttgattgagc atccccacag gattggcgat ctcctggaat ccggcaggtg gaatggagaa
     3241 cacgcggtcg tcatttttca ccgcgtgcgg atactgagcc agaatggcct tgcccgtctc
     3301 ttcatcagca tataccggca ccagcggtga gaaagtcagg gcacttccca gaatggaacc
     3361 gtactcggag ttggtctcga tgccggtgga tttattgcgg gaataaccca catttacacc
     3421 cagttgtacg ttgtttagaa aactgcgctc cttcgtttcg aaaatattgt agatcgagtt
     3481 ggaacgcaaa ctgtaacgct caaaatttga tttgtcgtaa tttccgccca cgatacctgc
     3541 ctgatcgaaa tagccgaacg agaggaagta atttcctttg tccgttcccc cggagacact
     3601 cacctgatgg ttctgtacgg gagcattgta gtaaaaggtt tcatcctgcc agtcggttcc
     3661 ttttccggca cccatcactt gctcgttgtt gtaacgaggc aggttgccat cgttgatttg
     3721 ggcctcgttc atgataacca tgtattcccg ggcgtccaga atttcctttt tcttccaggg
     3781 attctgccat ccgtaactca catcgtagtt gaccgttgcc ttacccttat cccctttttt
     3841 ggtagttacc aggatcacac cgttggcggc ccttgacccg tagatggctg ccgaagctgc
     3901 atccttaagg atttccaccg aggcgatatc gacggggttc aggtagttga tgcctccacc
     3961 cacttccata ccgtccacga tataaagcgg ttcgctgttg ttaatggttc cgataccgcg
     4021 gatccgtacc ttggagtccg accccggctg gccggagctc tgggtgatct gtacacccga
     4081 caccttgccc ttcagtgcgt cttccacgcg ggcaggcctt gtcacgttca gttcctctgc
     4141 tgataccttg ctgattgccg cagtaaccac actcttgcgc tgtaccccgt aaccaacgac
     4201 caccaattca tccagcacct ctgtatcttc ctgcagttgc acattgagca cggtctgatt
     4261 ggcgatggtg aattcctgtc cggtgtaacc gatataggaa aagcgcagca cggatccccg
     4321 cggaagtgtc atgctgtaag tcccatccac atttgtgact gtccctatgg tagtccccgg
     4381 taccacaatg ttggccccga taattggttc cccattcaca tcagtcaccg ttccggttaa
     4441 cctcacctcc tgcaggggtg tttgccttgt tacggcattc acaggaaccg gttggtcaaa
     4501 aatataaatc ttgttgtccc tgatctcata cccaatctga cggggagtca gtaattggtt
     4561 aagtacttgc gcggcttccc catccgtaac ccgaatggtc acttcatcgc ttaaatccac
     4621 ggcctccttg ctgaatgcca gggtatggcc gatctgttcg gtaatgcttt ccagcactgt
     4681 tctcagggtt gccttgttgt agttgaggtt cactttctgt gcgaacagca tcgctgtacc
     4741 cagcacgaag aaggaaagca gcatcatcag gcgccttttc attggaattt ttcccatggt
     4801 tgttcgtatt acatattaaa aattaaaaaa ttaaaaattc tcccatctgt ttttgtacag
     4861 atatcaaatt aacaaaaaga gaatctgtta tggattcttc ataaggttga cacggcatgg
     4921 cgaaaaaagt atgaggaaag attaaaaatt aacaaaataa atggggtaaa atgaaaaaca
     4981 acctacacct tgccttctaa aacactcatt tacttaacgt aagcgagtat cttttctctg
     5041 caatttgcaa attatttaac agcatacaaa acaaaaaggg agagtaaggg ggatattgtg
     5101 atcaaatcga tacaaacaca ttgtcacctt tatattggaa cttcaccggg gcaattttct
     5161 gcaactcgtt caacagatct tccaaatgga gttgtttggt ggtaagtgta taactgaaat
     5221 taagcgcttc gggcgattgt acatgaaaat gcacgttgta ttttctctct acggttttta
     5281 taacctcagc aagtggggtg tcgtagaagt aaagcacatt tttcctccat agcgaaacat
     5341 tggaaggctt ggaaagatta taaagcgatg tttttcccgt ggttttattg tattcaatct
     5401 gttgccccgg agacatggga tatttacttt gctgtacctg aaaggatgtt tttccctcat
     5461 ccagtacgac ctgaatagtt tcactttcgc tatatgcatt cacattaaaa gcggttccca
     5521 atacctcaac cgacgtattc tgggcgtgca ctacaaaagg gcgttttcta tcgggcgcaa
     5581 cattaaaata tgcctcaccg tccagatata cctccctgtg cctgaatcca aagtgttccg
     5641 ggtaccgaat cttcgtatca gcattaagaa aaacctctgt tccgtcctga aaaaatattc
     5701 gggcatcttc tcctttcggg atatagagtt cggcataggt tgtgccctga aaaaaggagg
     5761 tttgtgaatg gataaaatag cccaagccca gtaaaaacag aatcggaaca agaaccgcag
     5821 cgacattacg agtaactgtt cttagacgtt taatacggat atctttatcg attcttctga
     5881 aaagcagatc cgactgaagt ggagtaaaag actttccgct atcggcatca gcatccatca
     5941 aatatgcatc tctatcaagc atatcagaca gacattgttg accttcaata gtagatgaaa
     6001 accacccagc cacttccttt gcctcctctt tagacgcagt ttcactaatg actctgttta
     6061 ttattttact gtttgttgac atcaccgaca ttaacgctta ccttatttga cacatttccg
     6121 ggcaaaagat atgagttgaa tcttgttttt atcatgtcat catcttgatc tgctgaaaat
     6181 atgaccttaa tatttttact gactcct
//
LOCUS       DAOSDR010000184         6182 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrimonas sp. isolate AMR_MDS_1826
            NODE_4571_length_6182_cov_4.045373, whole genome shotgun sequence.
ACCESSION   DAOSDR010000184 DAOSDR010000000
VERSION     DAOSDR010000184.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863355
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrimonas sp. (wastewater metagenome)
  ORGANISM  Petrimonas sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Dysgonomonadaceae; Petrimonas.
REFERENCE   1  (bases 1 to 6182)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6182)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:10:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,720
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 35
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6182
                     /organism="Petrimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4571_length_6182_cov_4.045373"
                     /isolate="AMR_MDS_1826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2023866"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(39..6176)
                     /gene="sprA"
                     /locus_tag="PLI30_08145"
     CDS             complement(39..6176)
                     /gene="sprA"
                     /locus_tag="PLI30_08145"
                     /inference="COORDINATES: protein motif:HMM:TIGR04189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell surface protein SprA"
                     /protein_id="HOI79560.1"
                     /translation="MTNKRSSYDQARDILALADLGEHSSIHNPLWSSTGTETVPHNDA
                     NTMYRQLISTYVAARDISQTAAVLPSTVIMGRDYEKIESARLLTPSEYTFQPQLGYVS
                     LRTPLQADEVLAVAYEYIYNGKAYQVGEFSSNQNVGALFLKLLKPVSLSPQAYTWDLM
                     MKNIYSLGYNAYNIQKDRFKLSITYQSDTTGVYLNYIPEGDIRDHLLLSVMNLDNLNS
                     ANDPHPDGIFDFLDGYTVMADNGRIIFPVVEPFGSHLRKMIADDAVAEKYVFQQLYDS
                     TLTVARQFAEKNKFRISGEYRGSSGAELNLNATNITRGSVRVTANGVALIEGTDYTVD
                     YISGTVIIINQAILDAGTPVTVTLENQSVFNMQRKTMMGVDLAYNFSKDFRVGATVMH
                     YYEKPLTVKTVFGEESVKNTLWGLNTSFRKQSYALTRLLDILPFVDASAPSQLTANLE
                     FAHMIPGHYRNKYTGGYSYLDDFETSTSRIDLRSPYGWSLASTPFNDTSTGLFPEAAL
                     TNNIDYGKNRAHMAWFYIDGLFTHRNSSLTPAHIKNDKEQLSNHLVREIYEREIFPDK
                     EAVYGDPPTLPVMNISFYPDERGPYNLDTSIDPEGKLLNPEKRWGGITRKMDIRDFEA
                     ANIEYIEFWLMDPFVNDKTGADRGGDLYFNLGEISEDVLKDGKKFFENGLPVNGDAST
                     SGTSIWGKYPLRQSTVYAFDNSQGDESRRVQDVGLNGLSTEEEKAFPTYAAYLDEIIP
                     KLSEEVLPRMRADRHSLLNDPSGDNFRHYRGQEQDRAQLSILDRYKYYNGTEGNSLAP
                     DETQPYSTASRTTPDVEDIDNDNTLNENESYYQYKVKLRPGNMTVGSNYIVDKREANV
                     SLRNGKTGKVNWYQFKIPLRDYQSRVGNMRGFNNIRFMRMFLTGFGQPVFLRFATLEL
                     VRSEWRTYTQSLQSGGTVAGPGKLEVSTVSIEENGDRTPVNYVLPPGVTRILDPSQPQ
                     LRQQNEQAIALKVKDLDPGDSRAIYKNTEYDLRRYKRLQMFIHAEETVDGSSDLQDDE
                     MSIFLRIGSDYRNNYYEYEIPLKLTPAGRYSSNIPDHQETVWKPGNKFDFPLELLTSL
                     KLKRNAEKRRGSGVTYLTPYSEADPQKPENRLTVVGNPSLAEVKVIMIGVRNNSVSAK
                     SSEVWANELRLSEFDEKGGWAVQGNVNLALSDIGSLTVSGRKETVGFGTLDQSLLERR
                     NDDYSSINVAMNMELGRFLPEPLKISAPLYYSYSNQTTAPQYDPLNRDILLSESLKNT
                     RNKQERDSVIRLAVTQTLNKSLILNNIKMNIKSKNPMPYDPTNFSFGYSYSENHFQSP
                     DTEYNNSIHQRLQANYGYTPLVKPFEPFKNFSFNYLPNNIQISSQLMRNYQETQLRDL
                     NAHMSGFSQSQRQYLTFSQFFTWDRDFSITWDLTRNLKTSFRSGTIAEIEEPYLQVNK
                     KLNRDDYELWKDSVIQSIQNLGKPLNYEQTADISYTLPFAQIPVLDWMSVSTAYNSRY
                     RWERGAFIRDENIGNILQNDLSLTVNGRLNLVQLYNKIGFLRKTGQRFDADVAQYLAR
                     SLMMVRSVNVNFGYRSRTDIPGFDPMVGDFFGQSHTPAGLIPGLGFAFGFDGGERFLE
                     KSDANNWLVKNADNISPALYQQTHNVRMEATLEPLRGLKIDLNALYENSRRTEIQYMF
                     DGMPKIYGGSFAISTLALASAFENSNARNDYASPSFDRFLANREVVAGRVRSRYQNST
                     YPNRGFIAETAFQNQPFSPENGDVNLHSADVLIPSFLAAYTGRDAQKIGLTAFPDLLS
                     LLPNWDISYNVLQMLPALRANFKSLLLTHKYVSQYRVGAFSSFLSWVPLDDTSDLGYV
                     RDVLTGSPVPSSPYDISAVNLIETFSPLIEARGVLDNNMTFNFRINHTRSLNLNIASY
                     QVVETNDNDMVFGLGYRLPDFNRIIGFGSNSVKAGRRQTRVNRAQVTQTENADNLPEF
                     NNDLNIRVDVSHKITQALIRKIEDRFTQATSGLKTTAIRFSADYALSRSLTLRAFFDK
                     TIHVPLVSSAAYPTANTSAGMSLRLNLNR"
BASE COUNT         1430 a         1434 c         1591 g         1727 t
ORIGIN      
        1 aggtgatgag cggggaatac gagccctaaa agtacccgct accggttcaa attcagccgc
       61 aggctcattc ctgcacttgt gttggctgtg gggtatgctg ctgaggagac caacggcaca
      121 tgaatggttt tatcgaagaa tgcacggagc gtgagtgacc ggctcaacgc atagtccgca
      181 gaaaagcgga tggcggtggt ttttaatccg ctggtggctt gggtaaaccg gtcttcgatc
      241 ttacggataa gggcctgtgt gattttgtgc gacacatcta cccggatgtt caggtcgttg
      301 ttgaattccg gcaggttatc ggcgttctct gtttgcgtta cttgtgcgcg gttaacacgt
      361 gtttgcctgc gaccggcttt taccgagtta gagccaaagc cgataatgcg gttaaaatcg
      421 ggtagtcggt aacctaaccc gaaaaccata tcgttgtcgt tcgtttccac gacttggtaa
      481 gatgcgatgt tcaggttgag cgaacgcgtg tggttaatgc ggaaattgaa ggtcatgttg
      541 ttatcgagta ccccccgtgc ctcgataagc ggactaaaag tttcgatcag gtttaccgcg
      601 gagatgtcgt atggtgacga cggaacaggc gaaccggtaa gcacatcgcg gacatatccc
      661 aaatcggacg tatcatccag gggtacccag ctgaggaacg aggagaacgc tcctacacga
      721 tactgcgaca catatttgtg cgtaagcagc agcgatttga agtttgcccg gagcgcaggc
      781 agcatttgta aaacgttgta cgaaatgtcc cagttgggca gtagcgatag caggtcagga
      841 aaagcggtca atccgatttt ttgagcatcc cgtcccgtgt aggcggccag gaaagagggt
      901 atcagcacat ctgccgaatg gaggttcacg tcgccgtttt ccggactaaa gggctggttt
      961 tgaaatgccg tttcagcaat aaacccccga ttggggtagg tggagttttg gtaccggctg
     1021 cgtaccctgc cggcgacgac ttcccggttt gccaggaacc ggtcgaacga tggcgaggcg
     1081 taatcatttc tagcgttgga gttctcaaaa gccgaagcca aggctaatgt acttatcgca
     1141 aaacttccgc cgtaaatttt gggcattccg tcgaacatat attggatttc cgtacggcgg
     1201 ctattttcat acagcgcatt taaatcaatt ttcaggccgc ggagcggttc aagtgtggcc
     1261 tccatacgca cgttatgtgt ttgttggtat agagccggcg agatattgtc tgcgttttta
     1321 actaaccagt tgttggcatc agacttttcc agaaaacgtt cacctccatc aaatccaaaa
     1381 gcaaagccca gcccggggat cagtcccgca ggcgtgtggc tttgtccgaa aaaatcaccg
     1441 accataggat caaaacccgg aatatctgtc ctggacctgt acccgaaatt tacgttcaca
     1501 ctgcgcacca tcatcaggct gcgtgccagg tattgggcaa cgtctgcatc aaagcgttgt
     1561 ccggtttttc gcagaaaccc tattttgttg tacagctgta ccaggttcag ccgtccgttt
     1621 acggtgagcg acaggtcgtt ttgcaaaatg tttccgatat tttcatcgcg gataaacgca
     1681 cctcgttccc accggtaacg cgaattgtat gctgtgctta cactcatcca gtccagaaca
     1741 ggaatttgcg caaacggaag tgtgtagctt atatctgccg tttgttcgta attgagtggc
     1801 tttcccaggt tctgaatact ctgaatgaca gaatctttcc aaagctcata gtcgtcgcgg
     1861 ttaagttttt tgtttacctg cagataaggt tcttctattt cagcaatggt gcccgaacgg
     1921 aacgaggtct tcaggtttcg ggtcaggtcc caggtaatcg aaaaatccct gtcccaagtg
     1981 aagaattggc tgaacgttaa atactgccgt tgcgactgtg aaaatcccga catgtgtgcg
     2041 ttcagatcgc gtagctgcgt ttcctggtag tttcgcatca gttgggaact gatctggata
     2101 ttgttgggga ggtagttgaa actgaaattt ttaaaaggtt caaacggttt cacaagcgga
     2161 gtgtaaccgt aattcgcctg caagcgctga tggatgctgt tgttgtattc ggtatcgggg
     2221 ctttgaaaat ggttctcgct gtaggaatat ccgaaactga aattcgttgg gtcgtacggc
     2281 atcggattct tactctttat attcattttt atattgttca gaatcaaact tttgtttagt
     2341 gtttgtgtga cagccaggcg aataaccgaa tcccgttctt gtttgtttcg cgtgtttttt
     2401 aacgattccg aaagcaaaat atcccggttg agcggatcat attgtggtgc ggtagtttga
     2461 ttggaatagg aatagtagag gggagccgat atctttaacg gctcaggtag gaatcgtccc
     2521 agttccatat tcatggcgac atttattgat gaataatcgt cgttccgtct ttccagcaag
     2581 ctttggtcaa gtgtaccgaa gcccactgtc tcctttcttc ccgaaaccgt taacgacccg
     2641 atatccgata acgcgagatt aacgttcccc tgtaccgccc aacctccttt ctcgtcgaac
     2701 tcgcttaatc ggagttcgtt tgcccacact tcggaagact tggcgctgac ggagttattt
     2761 ctaacgccga tcattatcac tttcacttcg gcaagtgacg gatttcccac aacggttaac
     2821 ctgttctccg gcttttgggg atctgcttcg gaataaggag taaggtaggt cacaccgctt
     2881 ccacgacgtt tttccgcatt gcgtttcagt ttcaggcttg tcagcagttc aagcggaaaa
     2941 tcgaacttgt tcccgggttt ccataccgtt tcctggtgat cggggatgtt cgaactgtag
     3001 cgtcctgcag gagttaattt aagtggaatt tcgtactcgt aataattgtt ccggtaatca
     3061 gaaccgatac gtaaaaaaat actcatctcg tcgtcttgca gatcgctgct gccgtctacc
     3121 gtttcttcgg catgtatgaa catctggagg cgtttgtagc gacgcaggtc gtactcggtg
     3181 tttttgtaaa tggcgcgtga gtcgcccgga tcgagatcct tcactttcaa tgcgatggct
     3241 tgctcgtttt gttgacgaag ttgcggctgg ctaggatcga ggatacgtgt cacacccgga
     3301 ggtaaaacgt aattcacggg agtacggtct ccgttctctt cgatgcttac ggttgagact
     3361 tccagcttac ccggtcccgc tacggttccg cctgattgaa ggctttgagt gtatgtccgc
     3421 cattcactgc gcacgagttc cagcgtggca aaccgaagaa atacaggttg tccgaaaccg
     3481 gtaagaaaca ttctcataaa ccggatgttg ttgaatcccc tcatattacc tacccgggac
     3541 tggtaatcac gtaggggaat cttaaattga taccagttca cttttcccgt ttttccgttg
     3601 cgcaacgaaa cgtttgcttc tcgtttatca acgatatagt tgctacccac tgtcatgttt
     3661 ccgggacgaa gcttcacttt atactggtaa tacgactcgt tctcattcag cgtattgtcg
     3721 ttgtcaatat cttccacatc gggtgttgtg cgcgatgcgg tggagtaggg ctgtgtttca
     3781 tcgggtgcga gagaatttcc ttcggtcccg ttgtagtatt tataacggtc cagaatgctc
     3841 agttgcgccc ggtcctgttc ttgtcccctg taatgcctga aattgtctcc tgaagggtcg
     3901 ttgagcaatg aatgtctgtc tgcccgcatt cttggcaata cctcttcaga aagtttagga
     3961 ataatttcat ccaaataggc ggcgtaggta ggaaacgctt tttcttcttc cgtactcaat
     4021 ccgttcaggc ccacgtcctg tacacggcgc gattcatctc cctgcgaatt atcgaaagca
     4081 tagacggtgg actggcgcaa ggggtatttt ccccaaatac tggtaccgga ggtactcgca
     4141 tcaccgttaa caggtaatcc gttttcaaaa aacttttttc catctttcag cacgtcttcc
     4201 gaaatttcac ccaggttaaa atacaaatcg ccgccacggt cagctcccgt tttatcgttc
     4261 acaaacgggt ccatcagcca gaattcgata tattcgatgt tagccgcttc aaaatcgcga
     4321 atgtccattt ttcgtgtgat tcctccccac cgcttttccg ggttaagtaa ttttccttcg
     4381 ggatcgatgc tggtgtcgag gttgtatggg ccgcgttcgt cggggtaaaa agagatgttc
     4441 atcaccggaa gtgtaggggg atcgccgtaa acggcttcct tgtcgggaaa aatctcacgt
     4501 tcgtagattt cgcgtaccag gtgattggat agctgctcct tgtcgttttt tatgtgtgcg
     4561 ggggtgagtg aggagttccg gtgtgtaaac agcccgtcaa tatagaacca cgccatgtgc
     4621 gcccggtttt ttccgtaatc gatattgttg gttaatgccg cttcgggaaa cagcccggtg
     4681 gaggtatcgt tgaatggtgt tgatgccagc gaccaaccgt agggtgaccg taagtcgatt
     4741 cttgaggtag atgtttcaaa atcgtccagg taagaatatc cgcctgtgta cttgttgcga
     4801 taatgtccgg gaatcatgtg ggcaaattcc aggttggctg tcagttggga tggggcggag
     4861 gcgtccacga agggcagtat atcgagcagg cgggttagcg catacgattg tttcctaaac
     4921 gaggtattca ggccccaaag ggtgtttttt acagattctt cgccaaaaac ggttttcacg
     4981 gtaaggggtt tttcgtaata gtgcataacg gtagctccaa ccctgaaatc tttcgagaag
     5041 ttgtacgcca ggtcaactcc catcattgtt ttacgctgca tgttgaacac cgattgattt
     5101 tcgagtgtca cggtcacagg tgttcccgca tccaggatcg cctggttgat gataatcacc
     5161 gtcccggaaa tataatccac cgtatagtcg gtgccctcga ttaacgctac gccgttagct
     5221 gtgacccgca cggatccgcg cgtaatgttc gtggcattca ggttgagttc cgcgcccgaa
     5281 ctgccccggt attcgcccga aatcctgaat ttatttttct ctgcaaactg ccgggcaacg
     5341 gtcagtgtgg agtcataaag ttgttgaaaa acgtattttt cagcaacggc atcgtcagcg
     5401 atcatttttc gcaagtgtga accgaaaggt tccactacag gaaagatgat ccgtccgtta
     5461 tcggccataa cggtgtaacc gtccagaaag tcaaaaatac cgtcaggatg agggtcgtta
     5521 gctgaattca ggttgtccag attcatcacg ctcagcaata ggtgatcgcg gatatcgcct
     5581 tcgggaatat aattcaggta aacgccggtg gtgtcgctct ggtaagtgat actgagctta
     5641 aaccggtctt tctgtatgtt gtacgcattg tatcccagtg aatagatatt tttcatcatc
     5701 aaatcccagg tataggcttg cggagaaagg gagacaggct tcaagagctt cagaaaaagg
     5761 gcaccaacgt tttggttgga tgaaaattcc cccacctgat aagcttttcc gttgtagatg
     5821 tattcgtagg caacagctag tacttcatcg gcctgcaacg gtgtacgcaa cgaaacataa
     5881 cccaattgcg gctggaaagt atattcggaa ggagtgagca gccgtgcgct ttcaattttc
     5941 tcgtaatcgc ggcccataat cacggttgaa ggaaggactg ccgcggtttg gctgatatcg
     6001 cgtgcggcaa cgtaagtgga gatcagctgg cggtacatcg tattggcatc gttatgcggc
     6061 acagtttctg taccggtaga cgaccacagg ggattatgaa tggagctgtg ctcccccaga
     6121 tcagctaaag ccagtatgtc ccgtgcctgg tcatagctgc ttcgtttgtt ggtcacccat
     6181 ac
//
LOCUS       DAOSDR010000185         6159 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrimonas sp. isolate AMR_MDS_1826
            NODE_4590_length_6159_cov_4.088139, whole genome shotgun sequence.
ACCESSION   DAOSDR010000185 DAOSDR010000000
VERSION     DAOSDR010000185.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863355
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrimonas sp. (wastewater metagenome)
  ORGANISM  Petrimonas sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Dysgonomonadaceae; Petrimonas.
REFERENCE   1  (bases 1 to 6159)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6159)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:10:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,720
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 35
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6159
                     /organism="Petrimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4590_length_6159_cov_4.088139"
                     /isolate="AMR_MDS_1826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2023866"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            481..1515
                     /locus_tag="PLI30_08150"
     CDS             481..1515
                     /locus_tag="PLI30_08150"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007653719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HOI79561.1"
                     /translation="MEFKNIQPIHILLLLFLTLVIQTGCKPGDSSDNLTPEVTTEDID
                     TIPITEVKKEWTAADIQLTENLEYDQYTLEDEYPYKDTVRRFQWEKVKEKIAHLENAM
                     ENGGNWGVFSNYKNMNGEAPTIEGFVRNEYKRVSDEYGVERYQSVPLYDVTQTDKMLR
                     YGRDGWLVKIPDSDTTELVRVQGVSFEGEYLVPNRYLKPWGNTIRFTKVVVVDVTNQH
                     VALLEKQGNTWNILSKNPATTGVHKPPYAQETPTGLFAIQQKKEKMYYQQDGSSQIAG
                     YAPYASRFTNGAYIHGVPVQLPRKNIIEYSASLGTTPRSHMCVRNASSHAKLVFERAT
                     VKESVVLVID"
     gene            1703..2248
                     /locus_tag="PLI30_08155"
     CDS             1703..2248
                     /locus_tag="PLI30_08155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007213010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein L,D-transpeptidase catalytic domain
                     family protein"
                     /protein_id="HOI79562.1"
                     /translation="MNLNGLIQFNAFEAAFTGYKKLNNNNSILTVIDFSLPSTEKRMY
                     VLDLEYKEVLFVSYVSHGRNSGENYATKFSNRNGSYQSSLGFYRTGSTYEGGNGYSLL
                     LDGLEKGINDMARSRAIVIHGADYCSETVIHSSGRLGRSFGCPALPRELTKPIIDAIK
                     GGSLLFIYADQPDYLQKSKVI"
     gene            complement(2403..2603)
                     /locus_tag="PLI30_08160"
                     /pseudo
     CDS             complement(2403..2603)
                     /locus_tag="PLI30_08160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004375684.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            3090..3734
                     /locus_tag="PLI30_08165"
                     /pseudo
     CDS             3090..3734
                     /locus_tag="PLI30_08165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004308773.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
     gene            3815..5092
                     /locus_tag="PLI30_08170"
     CDS             3815..5092
                     /locus_tag="PLI30_08170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010801068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOI79563.1"
                     /translation="MIEIISSIRKYNFWDNNPIDLGYSRTFYTDKIGQYLGNKLVKVL
                     VGQRRAGKSYILRQIASKLISEGVKSQNILYINKEYMELATLRTAVELEQLYKTYREA
                     LKPSGKVYVFIDEIQYIDEWERFVNSHSQDFAEPSELFISGSNSNLLSGELATLLSGR
                     YVEFEIFPFSFPEFCGISGLEINSGNYRKFLQSGALPELFNLPNDEMKQNYVSSIKDT
                     VMLRDIVARYNIKDVKLLDDLFVYLVNTASNLISITNIINFFASKKRKTNYETLSSYA
                     NYLENSFLVHRAERYNIKGKDTISGNSKYYLNDLAYRNYLYAGYGYGVGYLLENAVYL
                     SLRRAGYQVYVGTIKDTEVDFVAIKGDKRLYLQVTLSLTEEITIEREYRSLKLIDDNF
                     DKYVVSMDDYKIPTNEGIEHISAWELEDFLNSL"
     gene            complement(5284..>6159)
                     /locus_tag="PLI30_08175"
     CDS             complement(5284..>6159)
                     /locus_tag="PLI30_08175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008648492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HOI79564.1"
                     /translation="DNYVTAEKVKNEFLGHSENHATLLDLFVKHNNDVKQLIGISKSA
                     STYQKYEVTRKHLEKFLMYKYKLSDIALQEINLMFITDFEVYLKTIGKCNSNTTAKFM
                     QFFKRIILIARNNGIITGDPFANYKIRIQKVDIGYLTEEEIEKILKLNLVSERLEHVR
                     DLFIFSCFCGLAYIDVAGLRKEHIRKSFDGNLWIMTKRVKTGTNVNVPLLDIPKMILN
                     KYKNKLPNGKIIPIISNQKLNSYLKEIADLSRIRKNLTFHQARHTFATTTTLSKGVPI
                     ETVSKMLGHTNIERS"
BASE COUNT         1949 a         1211 c         1177 g         1822 t
ORIGIN      
        1 aagtccaacc gctaattaca agctaattgg acttattcat atctcattta ttgcggagag
       61 agggattatg aaccttctcc tttcctttat cattatcaat gatttaattt ttaaaaccta
      121 cagtcggcaa cgaattaggc acaattttac agcaccttat ttgtccactt ttgacctata
      181 ttctctgcat atatcttagg tttaaagata caattatttt tgtaataaat aactttcata
      241 acatcaatta agtatattta atttagcgca aggaaatgcg aatgctttat tctactggtt
      301 ctactacaga gcgactaccc gaccttctgg gcaagcgaga aaagctctga gctaccaaac
      361 aaattatgaa atagttatta aatgaatgat cattttgtac agtttggttg ttacatgaag
      421 taatcagtta cataaaacaa ttcaaattga aaaaaaccac acatataaca gaataaaccc
      481 atggagttca aaaatattca acctattcat attctccttc ttttattcct cacattggta
      541 attcaaacag gctgtaagcc cggggacagc agcgacaacc tcacacccga agtcactaca
      601 gaagacatcg acacaatccc cataacggaa gtaaaaaaag aatggacagc cgccgatata
      661 cagttgacag aaaacctcga atacgatcaa tatacactgg aagatgagta tccatataag
      721 gacactgtac gtcgctttca atgggagaaa gtaaaagaga agattgcaca ccttgaaaat
      781 gcaatggaaa atggcggcaa ctggggagta ttcagtaact ataaaaatat gaatggcgaa
      841 gctcccacca tagaaggctt tgtacgcaac gaatataaac gcgtgtccga cgagtatggc
      901 gtggagcgat accaatcggt gccgctttat gacgtaacgc aaaccgacaa gatgctacgc
      961 tacggcagag atggatggct cgtaaaaatt cccgatagcg ataccacaga gttagtacgt
     1021 gtgcagggtg tatcctttga gggagagtac ctcgtgccca accgttacct gaaaccctgg
     1081 ggcaacacca tccgctttac caaggtggta gtagttgacg tcaccaacca gcatgttgct
     1141 cttctcgaaa agcaaggcaa cacgtggaat atccttagca agaaccccgc caccaccggt
     1201 gtacacaaac ctccctatgc ccaggaaaca cccaccggtc tctttgctat tcagcaaaag
     1261 aaggaaaaaa tgtattacca gcaagatggc agctcccaaa ttgctggtta cgccccttat
     1321 gcgtcccgct tcaccaatgg ggcatacatc catggagtac cggtacagct acccagaaag
     1381 aatattattg aatacagtgc atcattgggc accacacccc gatcgcacat gtgcgtaaga
     1441 aacgcatcct cacatgcgaa actcgttttc gaacgggcaa ctgttaaaga atctgtcgta
     1501 ttggtaattg actgatggaa atattcagaa aaaaggtata tttgaaaatc actttctgaa
     1561 tttatctatg cgtacattca ctattactct gtttttggtc ctcttctgga acctctcttt
     1621 ccctattggt ggggattcct cgcatgggga tatagtaaaa cctaaactct tcgatcggga
     1681 ttgtgagctt ctctatgaag agatgaacct caacggattg attcaattta atgcctttga
     1741 agcagctttc acgggttata aaaagttaaa taataataat tcaatcctta cggtaattga
     1801 tttcagtctt ccttccactg agaaaagaat gtatgttctg gatcttgagt acaaagaggt
     1861 gttatttgtc tcctatgttt cacacggacg gaacagtgga gagaactacg ccactaagtt
     1921 ttcaaaccga aacggatcat accaaagctc tttgggattt tatcgtacag gcagcaccta
     1981 tgaaggagga aatggttact cacttctgct tgacggtttg gaaaaaggga tcaacgatat
     2041 ggcccgatca cgggccattg tgattcacgg agccgactac tgtagtgaga cagtaattca
     2101 ctcttccggg cgactgggac gcagcttcgg atgtcccgca cttcccagag agctgacaaa
     2161 gcctatcatc gacgccatca agggaggctc cctcctattc atttatgccg atcagcctga
     2221 ttacttgcag aaaagcaagg tgatttaagt cgtgcagaaa gggagagtta taaaccaacc
     2281 tcttgttccg atagatatca acaatttaat cattaaaacc tagaatcggc aacgatttat
     2341 acaaaacttt aaagcacctt atttatccac ttttgagcta tgctcttctg ctaaagtgaa
     2401 attcattttc ttttattatt ttgtgctttc tcatagatgt tttttctact attgagccaa
     2461 gtatctgctt cctcttgaat ttcaagttta gttctctttc tccctttctc tatccaaaca
     2521 agcaatttat ctttaaaaaa gtatagtttt ttcccattct tataacaagg aacctctcgc
     2581 ttcctgacaa gtacatacac agttgcttat atctctatat taaataatta tggaataaag
     2641 ttgaaggaaa tttcaccaaa tgaatatcca atgacagaga aagtagtagc aagaacactt
     2701 gtgtattttg caacgtgccg ttctcgttat agcgatacat agccgtttat ataaaatggc
     2761 aaatgctgga ggataatggt tgctggatat tcgaagtaac aagctgtggt cagaaattaa
     2821 ttcaggcact tccatgccgg caattcatta ctatttgcaa agtaacttat tttataatta
     2881 actggaaaaa aacggtgaaa tttggacaaa ttgttatcgt tttgtccaaa gcggaatcgg
     2941 tagtcggtaa cgatttaggc acgaaattgt atcttttggg gtctttttag tgttctttta
     3001 atcggacata aaagacacaa agtcatataa atcaatgact tgcaggcttc catcttgtta
     3061 ttatctggtt ttgtccagac cttccatcgg aggctctttc tgcctatgat agttctaaac
     3121 caaaactaaa aaactcactc aaaaaagtaa tttctcctac ttgggatgaa ctgacaaaat
     3181 ttagggaatt ccaaataccg gaacaaaaac aatacttgta gcgtgtccgt gatgggtttt
     3241 tattttgttg ctttaccgga ttaagatatt ctgatgtttt caatcttcac cgaagtgaca
     3301 taaaagaagg ccacattgaa gttactaccg ttaaaacagc cgatagtcta attattgagt
     3361 tgaacaatca cagcaaaacc attctcgaaa aatacaaaaa catcgcattt caagaacgaa
     3421 aagtactgcc tgttatcagc aaccaacgta tgaataccta cttgaaagaa ttggcggaat
     3481 tcgcagaaat aaatgagcct gcgcctgaaa cctactataa tggaaatgag cgcattgatg
     3541 tggttacgcc aaaatatgct cttttaagca cgcacgccgc tcgtcgcact ttcatctgca
     3601 acgccctttc acttggcatt ccggcaaatg tggttatgaa atggacagga cacagtgatt
     3661 acaaagcaat gaaaccttat atcgacattg ccgacgatat agaagccaat gcgatgagca
     3721 aattcaatca gttacagcat aattattcga ataaaaacca agaatatttt caagtgtatt
     3781 tgaaaataat tatatttgcg aaaaatattc aattatgatt gaaataatta gttccattcg
     3841 aaagtacaat ttttgggata ataacccgat agatttggga tattctcgta cattctacac
     3901 ggacaaaatt ggtcaatatc ttggcaataa attggtaaaa gtacttgtcg gtcagcgtcg
     3961 tgcaggtaaa agttacatct tacgccaaat cgcatccaaa ttgatttccg aaggtgtaaa
     4021 gagccaaaat attctttaca tcaacaaaga atatatggaa ctggcgactt tgcgaaccgc
     4081 tgttgagttg gaacagcttt acaaaaccta tcgagaagct ttaaaaccca gcggaaaagt
     4141 gtatgttttt attgacgaaa tacagtatat cgatgagtgg gaacggtttg taaactcaca
     4201 ttcacaagat tttgcagagc cgagcgaact atttatcagc ggttcaaact ctaatctttt
     4261 atccggtgaa ttggcaacgc ttttgtcggg tcgatacgta gaatttgaga ttttcccgtt
     4321 tagttttccc gaattttgtg gaatttcagg attggaaatc aatagtggaa attacagaaa
     4381 attcctccaa agcggtgcct tgcccgaact tttcaacttg cccaacgatg aaatgaagca
     4441 gaattacgtg tcgtccataa aagatacggt gatgttgcgc gacattgtgg cgcgctataa
     4501 tatcaaggat gtaaagttgt tagacgattt gtttgtttat ttagtcaata ctgcatccaa
     4561 tttgatttcg ataaccaaca tcattaactt ttttgcatcg aaaaagcgta aaacaaacta
     4621 cgaaacgctt tcatcttacg ccaattattt ggaaaattca tttctcgttc atcgtgccga
     4681 acgctacaac ataaaaggca aagacacgat ttcgggcaac agcaagtatt atctgaacga
     4741 tttggcttat cgaaactatc tttacgccgg gtacggatac ggagttggat atcttttgga
     4801 aaatgcagtt tatttgagtt tacgacgtgc aggctaccaa gtttatgtag gaacaataaa
     4861 agatacggaa gtcgattttg tggcaataaa aggcgataaa aggctttatt tacaagtaac
     4921 tcttagcctt acagaagaaa tcacaataga aagagaatac cgttctttaa agctcattga
     4981 tgacaatttc gataagtatg tagtaagtat ggacgattac aagataccca ccaatgaagg
     5041 gattgaacat atatctgcgt gggaattaga agattttctg aatagcctat aatatgacca
     5101 aaatcgccga catccttccc cgatacacac aattcaacac gataattgcc gaagtttcga
     5161 acaggcgttc gattgagttt tctcaacaac aattcgttgc tgatttttac actcaattca
     5221 ataatatcca atcgtctgaa gcgatgttga ttgatttgac gatgcaaacc aaattggaac
     5281 gcttcaagac ctttcaatat ttgtatgacc taacattttt gaaacagttt cgatagggac
     5341 acctttactt aatgtggtag tggtggcaaa cgtatggcgt gcttgatgga aagtaaggtt
     5401 ctttctaatt ctggacagat cggcaatctc cttcaggtac gaatttagct tctggttgct
     5461 gattattggg ataattttcc catttgggag cttgtttttg tacttgttca ggatcatttt
     5521 gggaatatcc aataagggaa cattgacatt tgttccggtc ttcacccgtt tggtcataat
     5581 ccaaagatta ccatcgaacg atttacggat atgttctttt ctaagtccag ccacatctat
     5641 gtatgccaaa ccgcaaaaac aggaaaagat aaatagatca cgaacatgtt ccaaccgttc
     5701 ggaaacaaga ttaagtttaa gtattttctc aatttcttct tctgtaagat agcctatatc
     5761 aaccttttga atgcgaattt tataatttgc aaatggatct cctgttatta tgccattatt
     5821 gcgggctatc aggattatgc gtttaaaaaa ctgcataaat ttagctgtag tattggagtt
     5881 gcatttaccg atagtcttaa gataaacttc aaagtcggtg ataaacatta agtttatctc
     5941 ttgcaaagca atatcagaga gcttgtactt atacatcagg aatttctcaa gatgtttccg
     6001 tgttacttca tacttttggt aggtagatgc ggatttggat atgccaataa gctgtttcac
     6061 atcgttgttg tgcttcacga acaggtccag taatgttgca tgattctctg aatggccaag
     6121 gaactcattc tttacctttt cggcggtaac gtagttgtc
//
LOCUS       DAOSDR010000186         6131 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrimonas sp. isolate AMR_MDS_1826
            NODE_4619_length_6131_cov_3.440750, whole genome shotgun sequence.
ACCESSION   DAOSDR010000186 DAOSDR010000000
VERSION     DAOSDR010000186.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863355
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrimonas sp. (wastewater metagenome)
  ORGANISM  Petrimonas sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Dysgonomonadaceae; Petrimonas.
REFERENCE   1  (bases 1 to 6131)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6131)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:10:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,720
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 35
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6131
                     /organism="Petrimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4619_length_6131_cov_3.440750"
                     /isolate="AMR_MDS_1826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2023866"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2672)
                     /locus_tag="PLI30_08180"
     CDS             complement(<1..2672)
                     /locus_tag="PLI30_08180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014224599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0071702 - organic substance transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SusC/RagA family TonB-linked outer membrane
                     protein"
                     /protein_id="HOI79565.1"
                     /translation="MKLKTILILCFLLFLVGLSAQQPITVSGTVTEGATGDPAIGVTV
                     LVKGTTNGTVTGIGGDYTLSNVPANATLMFSYIGMLTVEESVNGRSVINISMLEDVQA
                     LDEVVVIGYGTARKRDLTGSIVSVSAGEIADRPSANPLASLQGKVAGVQIVNTGRAGQ
                     DPEIRIRGTNSINGYKPLYIVDGLFSDNISHINPSDVESMEILKDPSSLAIFGVRGAN
                     GVIIISTKKAKAGQTIVNLNTSFGFKEIYDRIDLTNAAQFKELYDEQRNNQGAQPYNY
                     SYWGADTDWQDEMFQSGFITNNNISITGSGDRSRFYMGLGYTSEEGSIQTEKFSKITV
                     NLSSEYNVTDFLKFGFQVNGAKSKTPDAKGVGSIVRAAPIAPTFYDYTDPLTDKTERL
                     LHTMPEFQRAQLWNPLVETYIRGNHNLGVNHRVAGNIYGELKLLENFTFKTTLSLDYA
                     INEARAFSPVVYFYNPDIPGKENVQDRQSISQVKSTEYAAQGDHILTYQNTFDDVHSL
                     TVMGGITTNYREFSLLEGERSELLDKIYFSVPNDNYDKWWLTALNSDGAKNQYDTDKN
                     HQWRRFTMSYLLRGLYSYDNRYLLNGSFRRDGSSVFRGVGNTWENFYSFGTAWVVSEE
                     DFMKEQRVIDYLKLKGSWGVLGTENTGRSVYPTYPELTSVGSAVFGPNENIIPGYTKI
                     YNVQDLKWEKTYSWEAGIEINLLNQRLRIEPVYYNKRTKDIIVLLDSRGGAFNSLENL
                     GDIENKGLELTALWNDKIGESGFTYSVGANLTSIKNKVLSLGRDEGDAIYEGMARTVA
                     GYPIAYFYGYQVNGIYQNNEDIRQSEPNNVFAVKPGDLKFKDVNGDGKIDQDDRTMIG
                     KPTPDYTYGLNVNLAYRGFDFGVDMMGVYGNEVYR"
     gene            complement(2887..5760)
                     /locus_tag="PLI30_08185"
     CDS             complement(2887..5760)
                     /locus_tag="PLI30_08185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI79566.1"
                     /translation="MKFFLVKQLALLLITISTFQACFAQDIPFVPPAFNYTTHNYNAG
                     NQNWAVAQGRNRVIYVGNDYGLLSFNGVNWKLTPLPSHLSVKSIFIDHASGKEKIYVG
                     SFEEFGFFQRDEKNELLYHSLKHLIKDFTFYNDEVWTINKVGNRIFFQTFSSYFIYNE
                     SDNSLTVEKPFPAPLYFFTAEDTLYAQFIDEHFYVFDGTRFRLLLTKDKFDNDQVVSV
                     LPFNGEIYLTTAKSGIFSFNLTTQKLSRRKTTVDNELKSETVNRVTSLSDSVLVLGTL
                     KHGLYALKTDGSLLWQLNRDNGLYNNTVLGLFTDEDKNLWAALDNGVSHIRTSSPLSF
                     FEPKNIHIGLVEDILVKDNQLYVATNQGIYTYANEQKKIYLLPDFNIQSWFIRNFDNQ
                     IITGNNTGTAFIVNNRKKELPEESTGGTDIKAMRIHNRDFLLESTYTALQVYLRNTEG
                     QWVYSHKIDNFFDLINQVEQDHAGNIWATHMYKGLYRLRLDDRLQRVVLREFYPALDS
                     TATATKPIRTMKLMGRMVFTNGNSFYTYDDIAQKITPFDPLNQELPQLTDTRNIVTIN
                     DTSFWFLRPEEYTLVKFSTGKYHISDKVPFSILNNPPNTGRGNMYVDKNNVSYFCLNG
                     GIGKYNLFDKFHVNLPGLEISKIQSYNRKIDTPNNLPVNKRNVINFSENNLTFEFQYP
                     DYSKKKFTIECFLENYDTRWIATAPDFTITYPNLPANSYILNSRVLNDMGEVLSTLSV
                     PFQIKNPWYKTTLALMVYLTLAGLLLTYLIRIYIRMVIKRKNKIFAQREKERIAQLAC
                     QEKLIAEMKSEKLQNELIYKSKELANATLMVINHQELLNKLKKEIQENIRTGKLYRSN
                     GSSLVKMIDSNLSGEDEWAFFQENFDLIHEHFFRKLTERYPVLTPGDLRLCALLRLNY
                     SSKEIAKMLNLTLRGVEAARYRLRKKLNLEEEENLVSFVINFT"
     gene            complement(5821..>6131)
                     /locus_tag="PLI30_08190"
     CDS             complement(5821..>6131)
                     /locus_tag="PLI30_08190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI79567.1"
                     /translation="LRFFGLFTTTDRRPLTDLQKEIVDREFRFQLKLADVEIERFDMK
                     DNYFTLLMLFPLDSDVRSGINAAVNEINQYGNFLDPKFLFTNVKILSDSEIEQLLEKK
                     "
BASE COUNT         1603 a         1313 c         1316 g         1899 t
ORIGIN      
        1 cggtagacct catttccgta gacacccatc atatcaaccc caaagtcaaa acccctgtaa
       61 gctagattaa cgttcaagcc gtaggtatag tcgggagtcg gcttcccaat catggtcctg
      121 tcatcctgat caatttttcc atctccgttt acgtctttaa attttaaatc tccgggtttt
      181 acggcaaata cattgttcgg ttctgactgc ctgatatctt cgttgttttg ataaataccg
      241 tttacctgat agccataaaa ataggcaatg gggtatccgg cgaccgttct tgccatacct
      301 tcataaatgg catccccttc atcacgacca agggacagaa ccttgttttt gatgctggta
      361 aggttagcac cgacggaata ggtaaatccc gattctccga tcttatcgtt ccacaaagcg
      421 gttaactcca gtcccttgtt ttcgatatct cccaggtttt ccaacgagtt aaaggctcct
      481 ccacgtgaat cgagcaatac aataatatcc tttgtcctct tgttgtagta aaccggctcg
      541 atcctcaatc tctggttgag cagatttatt tcaatccctg cttcccagga ataggttttt
      601 tcccatttta aatcctgaac attgtagatt ttcgtgtatc cagggattat gttttcgttt
      661 ggaccaaaca cagcgctccc cacagaagtg agttccggat aagtaggata aacactcctc
      721 ccggtatttt ctgttcccaa gactccccag gagcctttca gtttcaggta gtcaataacc
      781 cgttgctctt tcataaaatc ttcttcagac actacccatg cggttccgaa ggaatagaag
      841 ttttcccacg tatttcccac tccgcggaac accgatgaac cgtcgcgccg gaaagaaccg
      901 ttcaataaat agcggttgtc gtaactgtat aatccccgca ggagatagga cattgtgaac
      961 cgtctccatt gatggttctt atcggtgtcg tattgatttt tagctccatc tgaatttaat
     1021 gccgtcaacc accatttgtc gtaattgtcg ttgggtaccg aaaaatatat tttatccagt
     1081 aactcgctgc gttcaccctc cagcaatgaa aattcacgat aattggtcgt tataccaccc
     1141 atcacagtca gcgaatgtac gtcgtcaaat gtgttctgat acgtcaagat atgatcacct
     1201 tgcgcggcat attccgttga ctttacttgt gagatggatt gcctgtcctg aacattttcc
     1261 ttgcctggaa tatcgggatt ataaaaatag acgaccggtg aaaaagcacg cgcttcgttt
     1321 atcgcataat ccagagacaa cgttgttttg aaagtgaagt tctcaaggag ttttaattca
     1381 ccgtagatat ttcccgccac cctgtggttc acccccaggt tgtgattgcc tctgatgtat
     1441 gtctccacca gtggattcca cagttgagcg cgctgaaatt ctggcatggt gtgcagcaga
     1501 cgctctgtct tgtcggtcaa cggatctgta tagtcgtaaa atgttggagc aatgggagct
     1561 gctcttacaa tggaccctac tcccttagcg tcgggagtct tgctcttcgc accgtttacc
     1621 tggaacccga acttcagaaa atcggtaacg ttgtattcgc tgctgaggtt tacggtgatt
     1681 ttcgaaaatt tctccgtttg gatggagcct tcttccgaag tgtaccccaa tcccatgtag
     1741 aacctgctcc tatctccgga tccggttata ctgatgttgt tgttcgtgat aaatccggac
     1801 tgaaacattt catcctgcca gtctgtgtct gcaccccaat agctgtagtt ataaggctgc
     1861 gcaccctgat tatttcgttg ctcatcatac agctccttaa actgagcagc atttgtcaga
     1921 tcgattctat cgtaaatctc tttgaaaccg aaagaagtat tcaggttgac aatggtttga
     1981 cccgcctttg cctttttcgt agagattata ataactccat tggcgccgcg tactccaaaa
     2041 atggccagcg atgacgggtc ttttaatatc tccatagatt caacgtccga tggattaata
     2101 tggctgatgt tatccgaaaa tagcccatct acaatgtata gaggtttgta tccgttaatg
     2161 gagtttgtcc ctctgatgcg aatttcagga tcttgtccag ctcgcccggt gtttacaatt
     2221 tgcactccag caacctttcc ttgcagcgat gctaatggat ttgccgatgg tctgtctgct
     2281 atttcaccgg cgctcacact gacaattgag ccggttaagt cccttttacg tgcggtgccg
     2341 tacccgatta ccactacttc gtccagggcc tgaacatctt ccagcatcga aatgttgatg
     2401 acggatcgtc cgtttacact ctcctcaacg gtcaacatac cgatataact gaacattaac
     2461 gtcgcattgg cgggtacatt agacagcgta taatctccac cgataccggt aaccgttccg
     2521 ttggttgtac ctttcaccaa tacggtaacc cctatcgcag ggtcgcctgt agctccttct
     2581 gttactgttc ccgagacggt aattggctgt tgggcggata ggccaacaag gaataacaga
     2641 aagcaaagaa tgaggatagt ttttaatttc atggattcaa atgcttttaa tttgttattt
     2701 aaataatcaa gtttctgagc caaattaacg tcatttttat gcgcatacat aataaagcac
     2761 ctcatttaaa ttacgttatg ttgtataaca tgtttttaaa gggtactgtt tatatacgtt
     2821 ttcatacata aaatacagta tatgagcagt ataaataagt gttattaata cgataaaacg
     2881 aagtggttaa gtgaaattaa tcacaaaaga taccaggttt tcttcttctt cgaggttaag
     2941 tttctttctt aaacggtatc gggcggcttc aactccacgc aatgtcagat tcagcatctt
     3001 tgcaatttct tttgatgagt agttaaggcg gagaagggca caaaggcgta aatcacccgg
     3061 tgttaatacg gggtatcttt cggttaattt gcgaaagaag tgttcgtgga taaggtcaaa
     3121 attttcttgg aaaaacgccc actcgtcttc tccagacagg ttactgtcga tcattttaac
     3181 gagactggag ccgttggagc gatataattt tccggttctg atgttttcct gtatttcctt
     3241 tttcagttta ttgagcagtt cctggtggtt gatgaccatc agggtggcat tcgccaattc
     3301 tttactcttg tatatcagct cattctgtag tttttcgctt ttcatctctg caataagttt
     3361 ttcctggcaa gccaactgag cgattctttc tttttcccgt tgtgcaaata ttttattctt
     3421 tctcttgata accatccgga tgtatatccg gataagataa gtgagcaaca atccggccag
     3481 tgtcaggtag accataagag ccagcgtagt tttgtaccag ggatttttaa tttgaaacgg
     3541 aacggaaaga gttgagagaa cttctcccat atcatttaac acccttgaat tcaatatata
     3601 ggagttagct ggcaaattgg gataggtaat ggtgaaatcc ggagcggttg ctatccaacg
     3661 ggtatcgtaa ttttccagaa agcactctat ggtgaatttt tttttgctgt aatcgggata
     3721 ttgaaactcg aacgtaaggt tgttctctga aaaattaata acattccgct tgttgaccgg
     3781 caggttgttc ggagtatcaa tcttacggtt gtaactctga atctttgaaa tctctagacc
     3841 gggcaagtta acgtgaaatt tgtcgaatag attgtatttc ccgatgccgc cgtttaaaca
     3901 gaaataggat acgttgtttt tatccacgta catgttccct cttccggtgt tgggagggtt
     3961 gttcagaatg ctgaagggaa ctttgtcgga tatgtggtat tttccagttg aaaatttaac
     4021 cagcgtgtat tcttcgggcc tgagaaacca aaatgaggta tcgttaatgg tgacgatgtt
     4081 cctcgtgtcg gtaagctgtg gaagttcctg attcaatgga tcaaaaggag tgattttttg
     4141 tgcaatatca tcgtacgtgt aaaaagagtt accattagta aacaccatcc ttcccattaa
     4201 tttcatggtg cggatgggtt ttgttgccgt ggcggtgctg tccaaggccg gataaaactc
     4261 ccgcaatact acccgttgta accgatcatc gagccgcaat cggtaaagac ccttgtacat
     4321 atgggttgcc caaatgtttc cggcatgatc ctgttcgacc tgattgatca ggtcgaagaa
     4381 attatcaatc ttgtggctgt atacccattg gccttcagta ttccggaggt agacctgaag
     4441 ggctgtatag gttgactcca acaaaaaatc cctgttgtga attctcatgg cctttatatc
     4501 cgtaccgccg gtactttctt caggaagttc tttttttcta ttgtttacga taaaggctgt
     4561 tcctgtattg ttaccggtaa ttatctggtt gtcaaaattc cgtataaacc atgattggat
     4621 attgaaatcg ggaagcaggt agattttttt ttgttcgtta gcataggtgt aaataccctg
     4681 attggtggct acatataatt gattgtcttt tacaagaata tcttccacga gccctatatg
     4741 gatatttttt ggttcaaaaa aagaaagagg ggaactggtc cggatatggg aaacgccgtt
     4801 gtccagagct gcccaaaggt ttttatcttc gtcggtaaac aaacccaata ccgtgttgtt
     4861 gtaaagtccg ttgtcccgat ttagttgcca aagcagggat ccgtctgttt tcaaagcata
     4921 aaggccatgt ttcagcgtgc ccaacacaag tactgaatct gataatgaag ttacccggtt
     4981 tacggtttca ctttttaact cattgtctac ggtcgttttt cggcgggata gtttttgggt
     5041 cgtgaggttg aaagaaaaaa tcccgctttt tgcagttgtc aggtaaattt ctccgttaaa
     5101 aggcaaaacc gagacaacct ggtcgttatc gaatttgtct tttgtcaaga gtaaacggaa
     5161 ccgggtccca tcgaaaacat agaagtgctc gtcgatgaat tgcgcgtaaa gcgtatcctc
     5221 tgccgtgaaa aagtagagag gagccggaaa gggtttctca actgtcagag agttgtcgga
     5281 ttcgttgtaa ataaaataag acgagaacgt ctgaaagaaa atgcggtttc ctaccttgtt
     5341 aatagtccag acttcatcgt tgtaaaacgt aaagtccttt atcaaatgtt tcagggagtg
     5401 gtacagaagt tcgttttttt cgtctctttg aaagaatccg aattcttcaa aagaccctac
     5461 ataaatcttt tccttaccgg acgcatggtc tataaatata gatttaacag acaaatgact
     5521 gggcaacggg gtcagtttcc agttcacgcc attaaagctc agcaagccgt aatcattgcc
     5581 gacgtagatc actcggtttc tcccctgtgc gacagcccag ttctgattcc cggcgttata
     5641 gttatgagtt gtgtagttaa aagccggagg aacaaagggt atgtcttgcg cgaaacatgc
     5701 ctggaaggtg cttatcgtaa tgagcagcag cgcaagttgt ttgacaagaa aaaatttcat
     5761 atttttcgcg cataattagt tccttactcg ttgcagtcgg gaataaccaa ggtgtatcgc
     5821 ctattttttc tccagtaact gctctatttc actatcagac aggattttta cgtttgtaaa
     5881 taaaaattta ggatcaagga agttgccgta ttggtttatt tcgtttacgg cggcgttaat
     5941 gcctgagcga acgtcggaat cgagaggaaa aagcattaac agagtgaaat agttatcctt
     6001 catgtcgaat ctctcaattt ccacatcagc caacttaagc tggaagcgaa attcccggtc
     6061 cacaatctct ttttgcaaat cggtcagggg acgacggtcg gttgtggtaa acaagccaaa
     6121 gaaacgaagt t
//
LOCUS       DAOSDR010000187         6068 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrimonas sp. isolate AMR_MDS_1826
            NODE_4672_length_6068_cov_4.442707, whole genome shotgun sequence.
ACCESSION   DAOSDR010000187 DAOSDR010000000
VERSION     DAOSDR010000187.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863355
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrimonas sp. (wastewater metagenome)
  ORGANISM  Petrimonas sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Dysgonomonadaceae; Petrimonas.
REFERENCE   1  (bases 1 to 6068)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6068)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:10:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,720
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 35
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6068
                     /organism="Petrimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4672_length_6068_cov_4.442707"
                     /isolate="AMR_MDS_1826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2023866"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..609
                     /locus_tag="PLI30_08195"
     CDS             <1..609
                     /locus_tag="PLI30_08195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019541169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HOI79568.1"
                     /translation="GGELKMGEVVNKVSEATGHKAVCVTDVGQHQMMATRYFKSTLTR
                     SMVTSGGLGTMGFGLPAGIGAKYGAPGRTVCIFVGDGGLQMSIQELGTILEYNIDVKI
                     ILLNNNYLGMVRQWQELFFDERYSETHLKNPDFVKIAEAYGIRGRKVTDRKQLDSAIE
                     EMLSHEGAYLLEAVVETKGMVYPMVPAGGSVTDILIGNGHKL"
     gene            622..>1167
                     /gene="ilvN"
                     /locus_tag="PLI30_08200"
     CDS             622..>1167
                     /gene="ilvN"
                     /locus_tag="PLI30_08200"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018108525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005948 - acetolactate synthase complex
                     [Evidence IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HOI79569.1"
                     /translation="MEGKTLYTVTIFSENTVGVLNQITTIFTRRQLNIETLSVSPSAL
                     AGIHKFTITVFAESEEAMKKLVRQIDKRIDVLKAYFNVDNELIHQELALYKLATDKIM
                     EHGSIEYLIRKYNIRVLEVNNDCVVFLKAGHYAETQGLFNELAEKIGVLQFIRSGRIA
                     ITKSKVERLSDMLAQREEMKQE"
     assembly_gap    1168..1267
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1347..2393
                     /gene="ilvC"
                     /locus_tag="PLI30_08205"
     CDS             1347..2393
                     /gene="ilvC"
                     /locus_tag="PLI30_08205"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008149840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004455 - ketol-acid reductoisomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HOI79570.1"
                     /translation="MAKMNFGGVEENVVTREEFPLSKAQEVLKNETIAVIGYGVQGPG
                     QSLNLRDNGFNVIVGQRKGGKTWDKAVADGWVPGETLFEIEEACEKATIIQYLLSDAA
                     QIEVWPTVKKHLTPGKALYFSHGFGITYKERTGIIPPADVDVILVAPKGSGTSLRTMF
                     LEGRGLNSSYAIFQDATGRAFDRVVALGIGVGSGYLFETTFKREVYSDLTGERGTLMG
                     AIQGLLLAQYEVLRENGHTPSEAFNETVEELTQSLMPLFAAKGMDWMYANCSTTAQRG
                     ALDWMTPFHDATKPVFEKLYKEVASGNEAQRSIDTNSKANYREGLEKELKALRESEMW
                     QTGAVVRKLRPENN"
     gene            2643..3005
                     /locus_tag="PLI30_08210"
     CDS             2643..3005
                     /locus_tag="PLI30_08210"
                     /inference="COORDINATES: protein motif:HMM:NF024949.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HOI79571.1"
                     /translation="MNDISRNDWNAMSDDHILSQIGEFVKHHRMEQNKTQAMLAEDAG
                     ISRSTLSLLERGDTVTVATLIRVLRVLDQLHIMDVFVVVRRQSPLALAQAERKKKQRV
                     SFPKRRKTDKSDNENIDW"
     gene            3015..4319
                     /locus_tag="PLI30_08215"
     CDS             3015..4319
                     /locus_tag="PLI30_08215"
                     /inference="COORDINATES: protein motif:HMM:NF019421.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HipA family
                     toxin"
                     /protein_id="HOI79572.1"
                     /translation="MMNTAIVNIWGKMAGAVAWDEKSGWASFEYDPAFKRLGWELSPL
                     KMPLSTEQRIYSFPELRKETGSSFDTFKGLPGLLADMLPDRYGNELINLWLAQQGRPE
                     NSMNPVEMLCFIGKRGMGALEIEPTILKQNVNTFSVEIDSLVEIASKMLSKKEPFVAN
                     LKSDEEKAMREILKIGTSAGGARPKAVIAYNEKTGEVRSGQTRAPEGFEHWLLKLDGV
                     SEVQLGATHGYGRVEYAYYLMALDCGIEMMPCRLLEENGRAHFMTRRFDREGSYTKHH
                     IQTFCAMKHFDYNRVNSYSYEQLFQTMRELKLTYAEAEQMFRRMVFNVIARNCDDHSK
                     NFSFILKQGDRWRLAPAYDLCHAYRPGSEWVSRHALSVNGKRENITREDLIVIGRSIR
                     NKKAEEIIDQVNDTVHNWNYYADKAGVDKDKKVSIDKTLISL"
     gene            complement(4336..4992)
                     /locus_tag="PLI30_08220"
     CDS             complement(4336..4992)
                     /locus_tag="PLI30_08220"
                     /inference="COORDINATES: protein motif:HMM:NF013247.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HOI79573.1"
                     /translation="MRKLSGKFICPLPKKFKPMFLETICIKNGTAVNLEAHVERMRKT
                     ANRFGFAAPVLPCLESLLPETLREKKVKCSIEYRETIRTVRFVAYTPKRIRSLVLVEA
                     EELDYSFKYSDRTQLEVLNNRGGVGDEVLIVQNGCITDTTYSNAVFRKNGEFFTPHTY
                     LLNGTKRQRLLRESIIKEAAITVDNLHEFEHVYLINAMLDIEDGMGCPVQSILNSIII
                     "
     gene            complement(4952..5890)
                     /locus_tag="PLI30_08225"
     CDS             complement(4952..5890)
                     /locus_tag="PLI30_08225"
                     /EC_number="2.6.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004337002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component I"
                     /protein_id="HOI79574.1"
                     /translation="MNEAGRTKTPFLFGLDFELSEGFFIENPLQQLEILFGMDGTGNV
                     TETTEGNPFAFVSFPENYSRYMRRFLHVMEGLRYGNSFLTNLTIKTPVETGLSLKEIF
                     LRSRAKYRLYVPGRFVCFSPERFVRIGKGKIFSHPMKGTIDASVKNAEETILNDYKEK
                     AEHCTIVDLIRNDISRIATRVKVSRFRYIDRVETNEGAILQVSSEIEGTLPGDYLENL
                     GTLFFELLPAGSVSGAPKEATVAIIREAEQEPRGFYTGVAGYFDGTGLDSCVLIRYIE
                     QTEGGFYFRSGGGITVNSECEKEYAEAVRKIYLPFT"
BASE COUNT         1668 a         1387 c         1475 g         1438 t
ORIGIN      
        1 ggaggtgaac tgaagatggg tgaagtggtg aacaaggtat cggaagctac gggacataag
       61 gcagtatgcg tgaccgacgt agggcaacac cagatgatgg ccacccgcta tttcaaatcc
      121 acgctgacac gcagcatggt cacttcgggc ggactgggca ccatggggtt cgggcttccc
      181 gccggaatcg gggcgaaata cggggctccc ggccgtactg tctgcatctt cgtcggagac
      241 ggtggcctgc agatgtccat ccaggagctc ggaaccatcc tggagtacaa catcgacgtc
      301 aagattatcc tgctgaacaa caactacctg ggcatggtcc ggcagtggca ggagctcttc
      361 ttcgatgaac gatactccga gacacacctc aagaatcccg acttcgtaaa gattgccgaa
      421 gcctacggca tccggggaag gaaagtgacc gaccggaaac agctggacag cgccatcgag
      481 gagatgctgt cgcacgaggg tgcctacctg ctcgaagcag tggtggaaac caaaggaatg
      541 gtttatccca tggtacctgc cggcggaagc gttaccgata tactgattgg aaacggacac
      601 aaactctaaa ctcaacaaat tatggaagga aaaacattat ataccgtcac cattttctca
      661 gaaaatacgg taggggtgct caaccagatc accaccatct tcacgcgccg gcaactgaac
      721 atcgaaaccc tgtcggtctc gccttctgcc ctggcaggca tccataagtt caccatcacc
      781 gttttcgcgg aatccgaaga agcgatgaaa aagctggtgc gccagatcga caaacgcatc
      841 gatgtgctca aagcctactt caatgtggac aacgagctga tccaccagga gctggccctg
      901 tacaaactgg ctaccgataa gattatggaa cacgggtcca tcgaatacct catccggaaa
      961 tacaacatcc gggtgctcga ggtcaacaac gactgcgtgg tgttcctcaa ggccgggcat
     1021 tacgccgaaa cgcaggggct tttcaacgag ctggccgaga agatcggcgt cctgcagttc
     1081 atccgctccg gcagaatcgc catcaccaag tcgaaggtgg aacgcctgag cgacatgctg
     1141 gcgcagcggg aagagatgaa gcaggaannn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1201 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1261 nnnnnnnaat aaaaatgtaa ataatcagac cggggaagca agggccggat gtgtctttgt
     1321 tcccggtttt aaaatcaata aaaaatatgg caaaaatgaa ttttggcggg gtagaagaaa
     1381 atgtagttac ccgcgaagaa tttccattaa gtaaagcaca ggaagttctt aaaaacgaaa
     1441 caatagcagt aatcggctac ggtgttcaag gtccgggtca atcgttaaac ctgcgtgaca
     1501 atggtttcaa cgtaatcgtc ggacaacgca aaggaggaaa aacctgggac aaagctgttg
     1561 ctgatggctg ggttccgggt gaaacgcttt tcgaaattga agaggcgtgt gagaaagcaa
     1621 ccattattca atatttgctt tcagatgcag cacaaattga agtttggcca acggtgaaaa
     1681 agcatcttac tcccggtaaa gcattgtact tctcacacgg atttggaatt acttacaaag
     1741 aacgcacggg aattattccg cccgccgatg tggatgtaat ccttgtagca ccaaaaggat
     1801 cgggaacttc gttgagaaca atgttcctgg aaggacgcgg gttgaactct tcttacgcca
     1861 ttttccagga cgctaccgga cgcgctttcg accgtgttgt tgcattggga attggtgttg
     1921 gatcgggtta tctgttcgaa acaacattta aacgcgaagt atactcagac ctcaccggtg
     1981 aacgcggaac attaatgggt gccatccagg gactcttact ggctcagtac gaagtactcc
     2041 gcgaaaacgg acacaccccg tcggaagcgt tcaacgaaac ggtagaagag ctcacacagt
     2101 cgttgatgcc gctttttgcc gcaaaaggga tggactggat gtatgccaac tgctcaacaa
     2161 ctgctcagcg cggtgcgttg gactggatga caccattcca cgatgcaaca aagcccgtct
     2221 tcgaaaaact ttacaaagaa gtggccagcg ggaatgaagc gcaacgctcc atcgacacca
     2281 actccaaagc caactatcgt gaaggtttgg aaaaagagct caaagccctc cgcgaaagcg
     2341 agatgtggca gaccggagcc gtagtaagaa aattacgtcc ggagaacaac taagggttct
     2401 atttttttat aaagaaggtt gtctcaaaat aaaaatttac cttttgagaa atgaatattt
     2461 gaatttgttc tcagatcaaa tcagataagt aataagtatc cgaataaaaa gagggtgaaa
     2521 tcacacattt tgtgatacac cccttttttt attatacgta atcactagtg tcctagtttt
     2581 attgattaaa aaatgatatc tttgtgttgt ttttcattca ctatacataa aaaacttctg
     2641 ttatgaatga tatatcacgc aacgattgga atgcaatgag tgatgatcac attctcagtc
     2701 aaatcggaga atttgtaaaa catcaccgta tggagcaaaa taaaacccag gcgatgcttg
     2761 ctgaagatgc aggaataagc cgctccacac tcagtttgct ggaaagaggt gacacagtaa
     2821 cggtcgcaac actcattcgg gtattgcggg tattggatca attacatatc atggatgttt
     2881 ttgtggttgt ccgccggcaa agtccgttag ccctggctca agccgagaga aaaaaaaaac
     2941 agcgggtttc ttttcctaaa aggaggaaaa cagataaatc agataatgag aatattgatt
     3001 ggtaaaattg gtaaatgatg aataccgcca tcgtgaatat atggggcaaa atggcaggag
     3061 ccgttgcctg ggatgaaaaa tccggatggg cttcttttga atacgatcct gcttttaaaa
     3121 gacttggatg ggaactgtct cccttaaaga tgccgttgtc cacggaacaa cgaatttact
     3181 ccttccctga attacgcaaa gaaaccggtt cttctttcga tacttttaaa gggttgcccg
     3241 gattgcttgc ggacatgctg cccgaccgtt acgggaatga attgatcaat ttgtggctgg
     3301 cgcagcaagg acggccggaa aacagtatga atcccgtaga aatgctttgt tttataggta
     3361 aacggggaat gggagcattg gagattgaac cgaccatttt aaaacaaaat gtaaatacct
     3421 tttcggtgga aatagatagc ctggttgaaa ttgcgagcaa gatgctttcg aagaaagagc
     3481 cttttgtagc caatttaaaa tccgacgaag aaaaggccat gcgggaaata ctgaaaatcg
     3541 gaacatctgc cgggggcgca cgccccaaag ctgtgattgc ctacaatgaa aaaacgggag
     3601 aagtccgttc cgggcaaacg cgtgctcccg aagggtttga acactggttg cttaaacttg
     3661 acggggtgag tgaagtgcaa ttgggagcga cccatggata cggacgtgtt gaatacgcgt
     3721 actacctgat ggctttggat tgcggaatag aaatgatgcc atgccggtta ttggaagaga
     3781 acgggcgcgc ccatttcatg acccggcgct ttgacaggga aggaagctat acaaaacatc
     3841 atatacagac tttttgcgcc atgaaacatt ttgattataa ccgggtaaac agttacagtt
     3901 atgaacaatt atttcaaacc atgcgggagt tgaagctgac ttatgccgaa gccgaacaga
     3961 tgttccgccg tatggtgttt aatgtgatag cccgcaactg tgatgaccat agcaagaact
     4021 tctcttttat tcttaaacag ggagaccggt ggagattggc cccggcatac gacctctgtc
     4081 atgcttatcg tcccggaagc gaatgggtaa gccgtcacgc gttgagtgtt aacgggaagc
     4141 gggaaaatat tacacgggaa gatctgatcg tgataggacg ttccatccgc aataaaaagg
     4201 cagaggaaat tattgatcag gttaacgata cggtacacaa ctggaattat tatgcagaca
     4261 aggcaggtgt ggataaagac aaaaaagtgt caattgataa gacacttatc tctttataag
     4321 tttttgtcgg aaagactaaa taattataga atttagaata ctctgcaccg gacagcccat
     4381 cccgtcttct atgtcgagca tggcgttgat caggtaaacg tgctcgaact catgcaggtt
     4441 atcaacagtg atggccgctt ctttgatgat ggattcccgc aataacctct gccgttttgt
     4501 cccgtttaac agataagtat ggggcgtgaa gaattccccg ttcttccgga aaaccgcgtt
     4561 gctgtaggta gtatcggtga tgcagccgtt ttgcacgatc agcacttcat cacccacccc
     4621 tccccggtta ttcagcacct ccagctgcgt cctgtcggaa tacttgaacg agtagtccag
     4681 ctcctccgcc tccaccagca ccagcgaacg gatccgtttc ggggtgtatg ccacaaaccg
     4741 gaccgtacgg atcgtttccc tgtactctat actgcatttc actttctttt cgcgaagcgt
     4801 ttcggggagt aacgattcga ggcagggcaa taccggggcg gcaaaaccga aacggtttgc
     4861 tgtttttcgc atccgctcaa cgtgagcctc caggttgact gcggtgccgt tctttatgca
     4921 aatggtttcg agaaacatcg gtttgaattt tttaggtaaa gggcaaataa attttccgga
     4981 cagcttccgc atactctttt tcacattcgc tgttgaccgt gataccgcct ccgctccgga
     5041 aataaaagcc tccctccgtc tgttcgatat accggatcag cacgcacgag tcgaggcccg
     5101 ttccgtcgaa atacccggcc actccggtgt aaaagccgcg cggctcctgt tccgcttcgc
     5161 ggataatggc gaccgttgct tccttgggcg ctccggaaac cgatcccgca ggcaacagtt
     5221 caaaaaaaag cgtccccagg ttttcgagat aatcgccggg cagtgtccct tcaatctcgg
     5281 agctgacctg taagatagca ccttcattgg tctccaccct gtcgatgtag cggaaacggc
     5341 tcaccttcac ccgggtggct atccggctaa tgtcgttgcg gatcaggtcg actatggtac
     5401 aatgctctgc tttctccttg taatcgttca ggatggtttc ttccgcattt ttcaccgaag
     5461 cgtcaatggt tcctttcatg ggatgggaga atatttttcc ttttccgatc cgcacaaacc
     5521 tttcgggaga gaagcataca aacctgccgg gaacgtataa ccggtatttc gcccggctgc
     5581 gcaggaatat ctctttcagc gacaatccgg tctcaaccgg tgtttttatc gtcaggttcg
     5641 tcaggaagga gttcccgtaa cgcagcccct ccatcacatg caaaaacctg cgcatgtacc
     5701 gggagtagtt ttcgggaaag gatacaaaag caaaaggatt cccttccgtt gtctccgtaa
     5761 cgtttcccgt cccgtccata ccgaaaagga tttccaactg ttgcaaaggg ttctcaataa
     5821 aaaagccttc gctcagttca aaatcgaggc caaacaaaaa aggcgttttt gtccgccccg
     5881 cttcgttcat gcttcgtttt atagtgtcgc acgaggtaat catcctgcaa aattaaccga
     5941 attcgatctt tttcccttat ttttgcatat aaaataagaa aatcgcatgg aaatgaataa
     6001 aaaagagtta cgcatcgttt ttatgggcac accctccttc gccgtggaat cattgcgggc
     6061 actggtcg
//
LOCUS       DAOSDR010000188         6033 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrimonas sp. isolate AMR_MDS_1826
            NODE_4704_length_6033_cov_3.855303, whole genome shotgun sequence.
ACCESSION   DAOSDR010000188 DAOSDR010000000
VERSION     DAOSDR010000188.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863355
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrimonas sp. (wastewater metagenome)
  ORGANISM  Petrimonas sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Dysgonomonadaceae; Petrimonas.
REFERENCE   1  (bases 1 to 6033)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6033)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:10:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,720
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 35
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6033
                     /organism="Petrimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4704_length_6033_cov_3.855303"
                     /isolate="AMR_MDS_1826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2023866"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..250)
                     /locus_tag="PLI30_08230"
     CDS             complement(<1..250)
                     /locus_tag="PLI30_08230"
                     /inference="COORDINATES: protein motif:HMM:NF013618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HOI79575.1"
                     /translation="MLIGSLLLITSLCAQCGPSVEPSTTKAIIQVESGGNLFVIGDNT
                     LKKSFFPKSKKEAVRLADYLLSQGHNLDMGLMQVNSCHL"
     gene            complement(269..787)
                     /locus_tag="PLI30_08235"
     CDS             complement(269..787)
                     /locus_tag="PLI30_08235"
                     /inference="COORDINATES: protein motif:HMM:NF021981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S26 family signal peptidase"
                     /protein_id="HOI79576.1"
                     /translation="MKINMRPLCIMLVVFLLLAAVEAAKSNTDRRYWLNLSHSEPVGL
                     YVVTPLHGNMKRGETILMKCPQELEKYLYGRKWLPEGWPMFKTVGGVPGDTFCVSDTG
                     FSVQGKVLGPVYSTDAKGLPMPVIRGCRTVQENHFLPVATGLKSSFDGRYFGDVSVSL
                     VIGTARPVLTFQ"
     gene            complement(784..1260)
                     /locus_tag="PLI30_08240"
     CDS             complement(784..1260)
                     /locus_tag="PLI30_08240"
                     /inference="COORDINATES: protein motif:HMM:NF022453.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2840 domain-containing protein"
                     /protein_id="HOI79577.1"
                     /translation="MEFLTKTKLLFEEKRRNVKLLFGVPVASEDLRFSEGYSRKNVYF
                     KPDSLFALELWIANDHGTVIWMVYVLRAVWPGETANRVPQVEPGAQILLHARGKGRVR
                     KVLLWLNHLQEEIEDLSGLSPEYFQSAHYSLKNGLEPYSPREPFGPVLEYLKDKAQ"
     gene            complement(1344..1571)
                     /locus_tag="PLI30_08245"
     CDS             complement(1344..1571)
                     /locus_tag="PLI30_08245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI79578.1"
                     /translation="MHNLTMKDIVEAQNKRRKGEPYPMAETMEEIDYLIARVRALEHD
                     LSTVHTDRVPEQYKAADERPEGDKELLEEKD"
     gene            complement(1589..1840)
                     /locus_tag="PLI30_08250"
     CDS             complement(1589..1840)
                     /locus_tag="PLI30_08250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI79579.1"
                     /translation="MGKTSAERQNEYRQRNIRDVEATKSRLSIVISDHAMLALRRLAK
                     HNGCTQVALLERLLLEEQRKTIDGMTDKEAIAFHHSVTV"
     gene            complement(2826..3692)
                     /locus_tag="PLI30_08255"
     CDS             complement(2826..3692)
                     /locus_tag="PLI30_08255"
                     /inference="COORDINATES: protein motif:HMM:NF021637.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HOI79580.1"
                     /translation="MSKKPKNAAKQLELFVSDILTWSPKADRHSLEHPFFSLSKRKDV
                     RIRNYESPDGSVKIEVTPSVKGLATIWDKDVIIYAVSVLRDALNKGEKLDRNKSINIT
                     AYNLLLSTNRGSGGKSYDDLELALDRLKGTVIKTNIPTGGTQKTEGFGLIEKYKIMRE
                     SNTNRMVGVELVLSDWLWDAAVAGDKDLLTINREYFELTGGLERRLYEMSRKHCGLQK
                     QWEISLQKLHKKSGSTSTIREFRRMIREIVNRNALPDYSLSHDAEKDILTVRNRIAAE
                     IELSLNGGETES"
     gene            complement(3975..4256)
                     /locus_tag="PLI30_08260"
     CDS             complement(3975..4256)
                     /locus_tag="PLI30_08260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI79581.1"
                     /translation="MAIPRKPKAPESLDDVIMSGKATQIDLKSAAAAPTEKKKVGRKK
                     LEPSKTVGFRLPLRLIDLIVANAEEKTAGNQSALIIRILEGKEKLEAIG"
     gene            complement(4261..4908)
                     /gene="parA"
                     /locus_tag="PLI30_08265"
     CDS             complement(4261..4908)
                     /gene="parA"
                     /locus_tag="PLI30_08265"
                     /inference="COORDINATES: protein motif:HMM:NF041546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family partition ATPase"
                     /protein_id="HOI79582.1"
                     /translation="MKILTVASQKGGVGKSTVACNIAVQASMEGIRVLLVDADSQGSS
                     MSFRSIRESDDIKAMSITTPTLHKDLPTLKGFDLVIVDAGGRDSATLRSAMSVADLLV
                     IPVLPSTYDIWATSDTLDALREIRGFKEVNARILVNRLFANTVMARDTLESLKEIEVD
                     APLLETKLFNREDYSKAIAAGKGVVEFNGSGKAAAEIKSLMTEIRSILSINKTEE"
     gene            complement(4956..5285)
                     /locus_tag="PLI30_08270"
     CDS             complement(4956..5285)
                     /locus_tag="PLI30_08270"
                     /inference="COORDINATES: protein motif:HMM:NF024051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TSCPD domain-containing protein"
                     /protein_id="HOI79583.1"
                     /translation="MTTKKPRPRSLPGVTFEMQTQCGKIYVTITRDGEGRPFEIFARF
                     GKAGGCGAAIFDGLTKILSYALRSGMEPDNIVKAFSGISCHLGGNTCLNAVAEAFNTF
                     HHDSNYV"
     gene            complement(5304..5696)
                     /locus_tag="PLI30_08275"
     CDS             complement(5304..5696)
                     /locus_tag="PLI30_08275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI79584.1"
                     /translation="MEKGMFDAADYISIYTRGDALQDGFLIDLSLVAKEEGFLVPVAV
                     TCEAYRKIRPHEESELSGQDLRGRLHDMFRMLRLHAIKERYAETDRLAFNFLMAENGN
                     SRMVELVFVVHPGDQGEPVMTIMLPHED"
BASE COUNT         1428 a         1504 c         1394 g         1707 t
ORIGIN      
        1 tcaggtggca gctgttgacc tgcatcagcc ccatgtcaag gttgtggccc tgggaaagca
       61 ggtaatcggc gagccggacc gcttcttttt ttgatttcgg aaagaaactt tttttcagtg
      121 tattgtcgcc gatgacgaaa agatttcctc cactctcgac ctggatgatc gccttcgtgg
      181 ttgacggctc cacggaaggg ccacattggg cgcatagtga ggtgatgaga agcaggcttc
      241 cgatcagcat tattctcccc cttccctgct actgaaacgt cagcaccggc cgggccgttc
      301 cgatgacgag agagacggaa acatcgccaa agtaacgccc gtcgaaggag cttttcaggc
      361 ctgtggcaac cggcaggaaa tggttttcct ggaccgtccg gcaacctctg atcacgggca
      421 tgggaagtcc cttggcatcg gtggaataga caggtcccag aaccttgcct tgaacagaaa
      481 aaccagtgtc cgaaacgcag aacgtatccc ccggaactcc tccaacagtc ttgaacatcg
      541 gccaaccttc cggcagccat tttctgccgt agagatattt ttctaattcc tgcgggcatt
      601 tcatcaggat ggtttccccc cgcttcatgt taccgtgtaa cggtgtcaca acgtaaagcc
      661 ctacaggttc gctatgggaa aggttgagcc agtagcggcg atccgtattg gacttggccg
      721 cctcgacagc tgcgaggaga aggaatacca caagcatgat gcacaacggc cgcatgttga
      781 tcttcattgt gctttgtcct tcagatactc aagtaccggc ccgaacggtt cacgcggtga
      841 atagggctcc agaccgtttt tcaggctgta gtgggccgac tgaaaatact cgggagacag
      901 tcccgacaga tcttcgattt cttcttgaag atggttcagc cagagcagaa ccttcctcac
      961 cctacccttc ccccttgcat gcaacaggat ctgggctcct ggttcaacct gtggcaccct
     1021 gtttgccgtt tcacctggcc atactgctct taaaacgtag accatccaga ttactgttcc
     1081 gtggtcgttg gctatccaca actccagggc aaacaggctg tctggtttga agtagacatt
     1141 cttcctgctg tacccttccg aaaaacggag atcttcgctg gcgaccggga cgccgaaaag
     1201 gagtttcacg tttcttcgtt tttcctcgaa caggagtttt gtttttgtaa gaaattccat
     1261 tttaaataat aacaaaggaa aatattctaa tcttacaggg taaaaaaatt tacctggtga
     1321 gtgctaaagg attttgtatg gatctagtcc ttctcttcaa gaagttcctt gtccccttcc
     1381 ggtctttcgt cggcagcctt atattgttcc gggacccgat ccgtgtggac tgtggaaaga
     1441 tcatgctcta gggctcggac cctggcaatc aggtaatcaa tttcttccat cgtctccgcc
     1501 atcgggtaag gttccccttt acggcgtttg ttttgcgcct cgacaatatc cttcatcgta
     1561 aggttatgca tcagaccgac tcaaaaagtt acacggtaac ggaatgatgg aaggcgattg
     1621 cttccttgtc tgtcatcccg tcaattgttt ttcgctgttc ttcaaggagc agacgttcaa
     1681 gcaaggcaac ctgggtacag ccgttatgtt ttgccaagcg ccgcagggcc agcattgcgt
     1741 gatcactgat gacgatggac agcctgcttt tggtagcttc cacgtcacgg atgttccgtt
     1801 gccggtattc gttctgtctt tccgcgctcg tcttgcccat aattttcctt tacgaactgt
     1861 taccgtgtaa cagacgtctt tgacatgtaa aaagaaggcc tttatttgcc ccctagatga
     1921 attttcattc tacagaagac cttaccaaag gctggagccg aaaaatccgc tcagaacgaa
     1981 aatgacggct tttttcgatc atctccaagc cagtctttag cagatgttga aaatatggcc
     2041 cataacgagc gttcattcgt ttttgtcatg gtgatttcat cctggttcgt aatattcctc
     2101 gttttcagtt cgcaatcctt cagaatcgaa atgtggcgtt aagagaagaa tctgagattt
     2161 ccaggtcaat tgacttaacc cggcaatcgg attttgcgga agccttgaga gaaactgggt
     2221 ttatccacat ggggaatcct gttaaaacct tttttagatc tatatttaaa accttttaca
     2281 cgttctataa agataagccc atggcggttt gacaggtttt tttgtgggca atattacgag
     2341 attgcgtgtg cattattacg aagtgagcgt gtttgatatt acgataggca ggtgggtaat
     2401 attacgagca cttcaacatg ttgtccacag tccggcccgt gaagggcgcc ggatttgtgt
     2461 atacgctgca agctgtgggt aatattacga taaaacctgc cctggaaacc tccggatgaa
     2521 aaatatcacg gtattgaata ttgttatgaa acctgttgat atttcaatga aaaataggaa
     2581 ctgtgggcaa tattacgata cggcctgtgt tattcattca gagctttaac tttatagtgc
     2641 caacgcgtct aacagactgc aaagaacttg aattaccagg ataaattgcc tatgtgggta
     2701 aaattacgaa acgaacgtgc tgactggaat attagaagca tgcctaaaag acctgctttt
     2761 taaattcgga tctgatcttt aaatgcatct ccctgtgtgt gatattacga acagaatagg
     2821 cgaggtcagc tttcagtttc tccaccgttt agcgaaagtt cgatttctgc ggctattctg
     2881 tttcgaaccg tcaggatgtc tttttccgcg tcgtgggaga gactataatc ggggagtgcg
     2941 ttgcgattaa cgatctctcg gatcatgcgg cggaattctc ggatggtcga ggtggaaccg
     3001 ctctttttat ggagcttttg gaggctgatt tcccactgtt tctgaaggcc gcaatgcttt
     3061 ctggacatct cgtacagcct gcgctccagg ccgcctgtca gctcgaaata ttcacggtta
     3121 atggtcagca ggtccttgtc gccggcaacg gctgcatccc agagccaatc gcttagaacc
     3181 agctctacac caaccatcct gttggtgttt gattcgcgca taattttgta cttttctatc
     3241 aggccgaagc cttcggtttt ttgcgtcccc cccgtcggga tgttggtttt tatgacagta
     3301 cccttcaggc ggtccagcgc gagctccagg tcatcgtagc tctttccccc gcttccccgg
     3361 ttggtgctta aaagcaggtt ataggcggtg atgttgatcg atttgttccg gtccagtttt
     3421 tcacctttgt ttaatgcatc cctgaggacc gataccgcat agatgatgac gtccttatcc
     3481 cagatggtcg ccaggccctt taccgacggg gttacttcaa tcttcaccga tccgtcggga
     3541 ctttcataat tgcggatccg gacgtctttt cttttggaaa gggaaaaaaa tggatgttca
     3601 agggagtgtc tgtcagcctt aggagaccag gtgaggatgt cggacacaaa aagttcgagc
     3661 tgtttggcag cattttttgg tttcttcgac atggcaccct cccctggttc gaacttttta
     3721 caacctcttt tttacaacgg aagttaccgc cgcaaaagca caaaatcccc gttgtgggta
     3781 atattacgat taatagcgtg ggtaaaatta cgaaaacggt gttcctgaac cagccgttgg
     3841 cttccagggc ctctcacagg acctggttaa atagcagggc atcccgtttt gatcattaat
     3901 aaaacatatc gaattgtatc gaaatatttc taataactat taaatcaatt aaaacgcttc
     3961 gaatatttat aatatcaacc aatcgcttcc agtttctcct tcccttccag tatccgtatt
     4021 atcagtgcgc tctggttccc cgctgtcttt tcttcggcgt ttgccacaat cagatctata
     4081 agccttaaag gcaaccggaa ccccaccgtt ttgctcggct cgagcttttt ccttcctact
     4141 tttttctttt cggttggtgc ggcggctgca cttttaagat cgatctgggt tgccttgccg
     4201 ctcatgatga cgtcatcaag agattcggga gcctttggtt ttctcggaat cgccatatct
     4261 ttattcctct gttttattaa ttgacaatat ggaccggatt tcggtcatca gtgattttat
     4321 ttccgcggcg gcttttccgc ttccgttgaa ctcaacaacc cctttaccgg cagcgattgc
     4381 cttgctgtaa tcttccctgt taaagagttt cgtttcaagc aagggcgcat ctacttcaat
     4441 ttccttcagg ctttcgaggg tgtcgcgtgc catgacggta ttggcgaaca gacgattgac
     4501 caggattctt gcattaacct ccttgaagcc gcgtatttcc cgcagagcat ccaatgtgtc
     4561 actggtggcc cagatgtcat aggtggaagg caaaacggga ataacaagga gatcggcaac
     4621 gctcatggcg cttctcagcg tcgcgctgtc ccggccgccg gcatcgacaa taaccaggtc
     4681 aaaacccttc agggtcggaa ggtccttgtg caacgttggc gttgtaatcg acatggcctt
     4741 gatatcgtcg ctttcccgga tggaccggaa agacatgctt gatccttgcg aatcagcatc
     4801 aacaagcagc acccgtatac cttccatgga ggcctgcacg gcgatattac atgctacggt
     4861 cgatttccct accccgccct tttggctggc tacggtcaat attttcatct ggcacctccc
     4921 gccggtttga aacatatcaa tttattttaa aaatatcaaa cataatttga atcatgatga
     4981 aatgtattaa aagcttcagc aacggcattt aggcaggtat tgccgccaag gtgacaggaa
     5041 attccggaaa acgccttgac gatattatcc ggttccatgc cggaccgcaa agcataagac
     5101 aggattttgg tgaggccgtc gaagatcgcg gctccgcagc ctccggcctt gccaaatctg
     5161 gcgaatatct caaacggcct accctccccg tctcgggtga tcgtcacata gattttcccg
     5221 cattgggtct gcatctcgaa ggtcaccccc ggtaaagatc ggggtctcgg tttttttgtc
     5281 gtcattgctt acgtcctcgg aaatcagtcc tcatgcggta gcatgattgt catgaccggt
     5341 tcgccctggt cgcctggatg gacaacgaag acaagttcca ccatcctcga attgccattt
     5401 tccgccataa ggaaattgaa agcaagcctg tccgtttccg cgtatctttc ctttattgca
     5461 tgaagacgca gcatccggaa catgtcatgc agtctgccgc gtaaatcctg gccgctgagt
     5521 tcggattctt catgcggcct gattttccgg tacgcttcgc aggttacggc aaccggtaca
     5581 agaaaacctt cctctttcgc aaccagtgaa agatcaatca ggaatccatc ctgcaacgca
     5641 tctcccctgg tatatatgct gatataatcg gccgcgtcaa acattccctt ttccatccgc
     5701 ccctcctttc aatacaattt atattgtatt gaataatatt aaaaagtgac aaaatgatta
     5761 gaaggtgtaa taaaaaattt gtaatatttt aaaataatat aaattaatat aaatgctatc
     5821 aaagtaagca taccgccaga ggcgggaact tcccccgcct ctggcggcgg cgacctatat
     5881 agccatttcc atttcccctg ccacagcacg gttcaggatg aaatcaaccg ccctctgggc
     5941 ctgacctgcc gccaggatca ccattgattt gtcgtttttc agttctttaa gccaggactg
     6001 tatatatgcg gcgctattgt cgatggtcgc ctg
//
LOCUS       DAOSDR010000189         6033 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrimonas sp. isolate AMR_MDS_1826
            NODE_4705_length_6033_cov_3.469053, whole genome shotgun sequence.
ACCESSION   DAOSDR010000189 DAOSDR010000000
VERSION     DAOSDR010000189.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863355
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrimonas sp. (wastewater metagenome)
  ORGANISM  Petrimonas sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Dysgonomonadaceae; Petrimonas.
REFERENCE   1  (bases 1 to 6033)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6033)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:10:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,720
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 35
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6033
                     /organism="Petrimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4705_length_6033_cov_3.469053"
                     /isolate="AMR_MDS_1826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2023866"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..937)
                     /locus_tag="PLI30_08280"
     CDS             complement(<1..937)
                     /locus_tag="PLI30_08280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019538157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI79585.1"
                     /translation="MYRLEIMNKGNAALKICLVLLCISSTVFAGEIDLSGSRLLVSPS
                     IKSPVKETIIQLLQEEIHRRTGIPLGVTDAWGGSPVIALALSSDGILNGKALPDTLPE
                     KTKLPLQAESFSVVVQEDAPRPVIWLVGADERGVLFAAGHLLRTSLLANKQILFDSSN
                     AVQSAPDYPIRGHQLGYRNTANSYDAWTKEQYEQYIRELAIFGTNCIETIPFDNTPSP
                     HMKLSKEEMNRHISTVCRNYGIDYWVWTPANVDLSDENLFRAEVKKHSDYYRQCPHLS
                     HVFVPGGDPGDNHPRHVMPFLKAIATELEKYNPRAG"
     gene            complement(951..3608)
                     /locus_tag="PLI30_08285"
     CDS             complement(951..3608)
                     /locus_tag="PLI30_08285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019538158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HOI79586.1"
                     /translation="MKISYIIIPLAGVFLLLSCIQKSTIEEFILTDNWYIHSAEGLDQ
                     DGAALSVSPVTDNWIQTSLPATVLGALCNAGIYEDPFLGMNLEEIPVEQFKKPWWYRT
                     SFDLTGFDSEKEQTKLLLDGINYSANVWLNGHLILSADSAFGAFRQFACDITSIALES
                     GNNLAIEISPPQPGDFYMGFVDWAPVPPDHNMGVYRPVRIKRSGKASIDRPFVTTHLN
                     TQTLDEATLSISAELTNHHSEPKKMTLEARIKNSVVSQTFTVEPGKTFLAELGPEKFK
                     ELTIKNPRLWWPNGLGNPEMYTMHLSLKEGNTLCDTATVRFGIRTIDTYLNEQNVRGY
                     KVNGREVLIKSAGWVDDLFLRDNTRSNRAQIRYVKEMNLNSLRFEGFWGTGREIYDLC
                     DENGILLMVGWSCQWEWPDYLGLEMEVPEEDENIPINEGVDKYGVKITPEEEQLLSDM
                     FRDQLLWLRNHPSIFVWAVGSDAMPKPSLEKKYAETMERYDKSRSLLVSAGDFTSELS
                     GVTGMKMNGPYEYVPPVYWFEDKKLGGAFGFNTETGPGPQIPPISSIRKMIPEEYLWP
                     PLNEMWDYHSGRKDFNSVKVYLNALNKRYGTAGDLEELAMKAQWANYEAIRPMFEAFV
                     VNRPVATGVVQWQLNSAWPEFYWQLYDWYLMPTGAYFGTKKACQPVNIIYNYHDRKIY
                     VANDILGDITDHTVRATLYDSQSKVLFEQGKKLSVQENSSVVFTELPELSDKKGTYFL
                     NLTLHDSQHKLVADNFYWLSGQEDQMDWDQYYWFYTPQKRYADFHELNDLPQPQVSVT
                     KKVYKEDDEWVVEVNLDNTSKNIAFGIELLLVDNQTENPVLPVYWSDNYVSLVNDDTR
                     TIRARCSLADAPSEPSVIIQGINVERIKK"
     gene            complement(3629..4873)
                     /locus_tag="PLI30_08290"
     CDS             complement(3629..4873)
                     /locus_tag="PLI30_08290"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019538159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HOI79587.1"
                     /translation="MRYNRLYSTIQTLSVTLFFFIASSFIAASPYPDRVVLTWAGNPS
                     NSQAVTWRTGRSVQRALAQIAVAEENGQDIRPTEIEATTTPFSSELGGEVCYHSVNFT
                     GLVPGTTYAYRVGDGDVWTEWFHFRTASSNPEPFSFIYFGDAQADIKTYWSRVFHQAF
                     RHAPYAAFTLHAGDLINRAENDEQWGEWFNAPGWVNASVPIIATPGNHEYSRDHHAES
                     VSPRLSSFWQPQFTFPQNGPEGVKESCYYIDYQGVRIISLNSNEKQEEQEQWLRDLLS
                     HNPQKWTIVTFHHPIFSPKAGRDNPELRSLWKPLFDEFKVDLVLTGHDHTYARTHSEG
                     TAYIVSVSGPKLYHLDKQEWMHRSAEKIQLYQIIRINSGELTYEAYTATGRLYDKFML
                     KKQEGATNLLTEAISDTTMPNE"
     gene            complement(4893..>6033)
                     /locus_tag="PLI30_08295"
     CDS             complement(4893..>6033)
                     /locus_tag="PLI30_08295"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HOI79588.1"
                     /translation="SFRETAYPASPEADQIVLTWCDDPATTQAIQWRTDTSVDKMTIR
                     YRSKESDKQEFSEAPASQQLLSDKYIHNNPVVKHWEVNITGLQPDNEYSYQIYNSDSG
                     KESPVYTFRTAPGEKSSFSFIHLGDTHNDDIVETVLKQAVKEVPDAAFLVHSGDHVNT
                     GLFRDLWDKYLHSGRDVFPRFSFVPTLGNHDSQDGLPPTLYTQLFMLPKDNACSLSPE
                     RNYAFSYGDARFFMIDATGDIEKIACWLEEELRQTKEKWKIVVTHFPPYVEDNSYLEI
                     RRSWCSLFDQYHVDLVLSGHVHQYFRSYPINNGEVVTDAKNGTIYLSSVVVEPRKPEP
                     PSEKYNEVYANKGGLFQVIRIDNNTLDFISKRFDGTIIDQFSLKK"
BASE COUNT         1476 a         1521 c         1386 g         1650 t
ORIGIN      
        1 ctcctgcacg gggattgtac ttttccagct cggtggcaat cgctttcagg aaaggcatca
       61 catgccgcgg atgattatcc ccgggatcac cgcccggcac aaagacatgg ctcagatgcg
      121 gacattgcct gtagtaatcg ctgtgcttct tcacttcggc cctgaacagg ttttcatccg
      181 acagatccac gttggccggt gtccataccc aataatcaat gccataattc ctgcatacgg
      241 tgcttatatg ccggttcatc tcttccttgg agagtttcat gtggggactg ggcgtattgt
      301 caaaaggaat cgtctctata cagtttgtcc cgaaaatagc caactcgcgg atatattgct
      361 catactgctc ctttgtccat gcatcgtagg aattggcggt attgcgataa cccaactgat
      421 gtccccggat gggataatcc ggtgcgcttt gtacggcgtt tgaagaatcg aacaggatct
      481 gcttgttggc aagcaaagat gtgcgcagca ggtgtcccgc ggcaaacaaa accccgcgct
      541 catcggctcc gacaagccag ataaccggcc ggggggcgtc ctcttgcacc accacgctga
      601 atgattcggc ttgcagggga agttttgtct tttcaggaag cgtgtcagga agcgccttgc
      661 cgttcaatat cccatccgaa gagagagcca aagcgatgac cggcgaaccg ccccaggcgt
      721 cggtaacacc taaaggaatg cccgtacgcc tgtggatctc ttcctgcaag agctgaatga
      781 tggtctcctt cacgggagat ttgatggaag gcgacacaag caacctgctg ccggaaagat
      841 cgatctcacc ggcaaaaacg gtggaggaaa tgcacaataa aacaaggcag attttcaacg
      901 ccgcattccc tttattcatg atctcaagtc tgtacatggc gaccagtctg ttattttttt
      961 atccgttcca catttattcc ctgtatgatt accgaaggtt cggatggcgc gtcagccagg
     1021 ctacagcgag ccctaatcgt tctggtgtca tcattcacca aagacacata attatcgctc
     1081 caatataccg gaagtacggg attttccgtt tgattgtcca ctaatagtaa ctctatgccg
     1141 aatgcgatat ttttactcgt attgtccaga ttcacctcca ccacccattc gtcgtcctct
     1201 ttgtacactt tctttgtgac actcacctga ggctgaggca ggtcattcaa ttcgtgaaaa
     1261 tcggcatatc gcttctgggg agtgtaaaac caatagtatt gatcccaatc catctggtct
     1321 tcctgacccg acaaccaata aaaattgtcg gcaaccagct tatgctgact gtcgtgcaag
     1381 gtcaaattca ggaaataagt ccctttttta tcggacagct ccggtaactc cgtaaaaaca
     1441 acggatgagt tctcttgcac ggacaacttt ttcccctgtt cgaatagtac tttagactga
     1501 ctgtcatata acgtagccct tacagtatga tcggttatat ctcccaatat atcgttggcg
     1561 acatatattt tcctgtcatg gtaattgtaa atgatattga ccggctgaca cgctttcttt
     1621 gtaccgaagt atgcgccggt aggcattaaa taccaatcgt aaagctgcca gtaaaattcc
     1681 ggccaggctg aattcaactg ccattgcacc actcccgttg ctaccggacg gtttacaacg
     1741 aatgcctcga acatcggccg gatggcttca taattcgccc attgtgcctt catggccagt
     1801 tcttccagat ccccggcggt gccgtatctt ttgtttaacg cattcaggta gactttcaca
     1861 ctgttgaagt ccttccgtcc cgagtgataa tcccacatct cgttcaatgg cggccaaaga
     1921 tattcttcgg ggatcatttt cctgatactg cttatcgggg gtatttgcgg tccgggacct
     1981 gtttctgtat tgaacccgaa agcgcccccc agttttttat cttcgaacca atagacggga
     2041 gggacgtatt cataagggcc gttcatcttc atccccgtta ctccagagag ctcgctggta
     2101 aaatcaccgg cagaaaccaa taacgaacgg cttttgtcat atctctccat tgtctcggcg
     2161 tatttctttt ccaatgaggg tttaggcatg gcatcgctgc ccaccgccca gacaaaaata
     2221 gaggggtggt tacgcagcca caacagctga tccctgaaca tatccgataa caattgttct
     2281 tcttcaggag tgattttcac gccatattta tcgacccctt cattgatggg aatgttctca
     2341 tcctcttccg gaacctccat ctccagaccg aggtaatcgg gccattccca ttgacagctc
     2401 catccgacca tcagcaggat accgttttcg tcgcagaggt catatatctc ccggcccgtc
     2461 ccccagaagc cttcaaaccg gagagaatta aggttcatct cctttacgta gcgtatctgc
     2521 gccctgttac tacgggtgtt atcacgaaga aagaggtcgt ctacccaacc ggcgctcttg
     2581 atcagcactt cccttccgtt tactttatat ccccgtacgt tttgttcatt gagataggta
     2641 tctatggtac ggataccgaa gcggaccgtc gccgtgtcgc agagcgtatt cccctctttc
     2701 agcgacagat gcatggtgta catttccgga tttcccagtc catttggcca ccagagacgg
     2761 ggattcttta tggtcaactc tttgaatttc tcagggccaa gttcggccag aaaggtctta
     2821 cccggctcca cggtaaatgt ctgactgacc acactgtttt ttatcctggc ttccagggtc
     2881 attttttttg gctcagaatg atgattggtc agttctgccg aaatggacag ggtggcttca
     2941 tccaatgttt gggtgttaag atgggttgtc acaaaagggc ggtctattga agcctttcca
     3001 ctccgcttga tccttaccgg ccggtacaca cccatgttat ggtcgggtgg gacaggcgcc
     3061 cagtcgacaa accccatata gaaatcaccc ggctgcggag gcgatatctc gatagccaga
     3121 ttatttcccg attccaaagc gatagaggtg atatcgcagg cgaactgccg gaaggctccg
     3181 aaagcactgt ctgccgaaag gatcaggtgg ccgttgagcc atacattggc gctataattg
     3241 atgccgtcca gcagcaactt cgtctgttct ttctccgaat cgaaccctgt caggtcgaaa
     3301 gaggtccggt accaccacgg ttttttgaac tgctcaaccg gtatctcttc cagattcata
     3361 cccaaaaagg ggtcttcgta tatgcctgca ttgcataatg cccccaacac cgtggcgggt
     3421 aaagatgttt gtatccagtt atccgtcacc ggagagacag ataatgccgc tccatcctga
     3481 tccaatccct cagcagaatg gatataccag ttatcggtca ggataaactc ttcaatcgtg
     3541 cttttttgta tgcaggataa cagtaagaaa acgcctgcta atggtatgat aatataactg
     3601 attttcatat gactatatct ctttctattt attcatttgg catagttgta tcagaaatag
     3661 cctccgtcag aagatttgtc gcaccttcct gctttttgag catgaacttg tcgtataacc
     3721 tccccgtagc ggtatacgcc tcataggtca gctcgccgga atttatacgg ataatctggt
     3781 atagctgtat tttctcggcg gaccggtgca tccattcctg cttgtcgaga tggtatagtt
     3841 tcggtccact gacggagaca atatatgcag tcccttcgga atgggtgcgg gcataggtat
     3901 gatcatgccc cgtcaagacc aggtcgactt taaattcgtc gaacaaaggt ttccacaaac
     3961 tgcgtaattc cgggttatca cgacctgctt ttggagaaaa tatcgggtgg tgaaaggtaa
     4021 cgattgtcca tttctgcgga ttatgggaaa gcaaatcccg taaccattgc tcctgctctt
     4081 cttgtttctc attcgaattg agtgaaatga tccgcactcc ctggtaatcg atataataac
     4141 aactctcttt tactccctcg gggccattct gggggaaagt gaactgaggc tgccagaagc
     4201 tgcttaaacg tggtgaaacg gattcagcgt ggtggtcacg agaatactca tgatttccgg
     4261 gagtggcaat tatcggcaca gaagcattca cccatccggg agcattgaac cattcacccc
     4321 attgttcatc attttcggcc ctgttaatga ggtctcccgc gtgaagcgtg aatgccgcat
     4381 agggagcatg tcgaaaggcc tgatgaaaga cacgtgacca ataagtttta atatctgcct
     4441 gcgcatcgcc gaagtaaata aatgaaaagg gttccgggtt ggaggaagca gtcctgaaat
     4501 gaaaccattc agtccataca tccccatctc ctacccgata tgcataagtg gttccgggga
     4561 ccagtcccgt gaagttcaca ctatggtaac agacctctcc acccagttca ctgctaaatg
     4621 gtgtggttgt cgcttctatc tcagtaggcc tgatatcctg tccattctct tctgccacgg
     4681 caatctgtgc cagcgcgcgt tgcacggagc gacccgtcct ccaggtgaca gcctgggagt
     4741 tggacggatt ccccgcccag gtcagtacaa ctctgtcggg ataaggagac gccgcaataa
     4801 aagaagatgc aatgaagaag aatagagtga cagagagagt ctgtatcgtg gaataaagtc
     4861 tgttgtagcg catctctttc atgtttgatt atttatttct tgagtgaaaa ctgatctatg
     4921 atcgtaccgt caaatcgttt ggatataaaa tcgagggtat tgttatcgat acggataacc
     4981 tggaacaatc ctcccttatt ggcatacact tcattatact tttcggatgg cggttcaggt
     5041 ttgcgcggct caaccactac cgaagagaga tagatcgttc cattttttgc atcggttact
     5101 acctccccat tattaatcgg ataggagcgg aaatattgat gtacatgtcc ggacagcacc
     5161 agatcgacat gatattggtc gaacagtgaa caccatgatc tgcgtatttc caggtaggag
     5221 ttatcctcca cataaggtgg aaagtgggtg acaactattt tccatttctc tttggtctgt
     5281 ctgagttctt cttccagcca gcaggcaatc ttttctatat cgcccgtagc atcaatcata
     5341 aaaaaacgtg catccccgta agagaaggca taattacgct ccggggacaa actgcatgca
     5401 ttgtccttag gcaacataaa cagttgtgta tagagcgtag gcggcaggcc atcctggctg
     5461 tcgtggttgc ccagagtggg aacaaaagag aatcggggaa aaacatccct tccggaatgt
     5521 aggtatttat cccataggtc cctgaacagc ccggtattta catggtctcc cgaatgtacc
     5581 aggaatgcgg catccggcac ttctttcact gcctgtttca acaccgtttc cacgatatcg
     5641 tcgttatggg tatcacccag atggataaag ctgaatgacg atttttcccc gggtgctgta
     5701 cggaatgtat aaaccggact ctctttcccg ctatccgaat tatatatctg ataactatat
     5761 tcattatcag gctgtaaacc tgtaatattt acttcccaat gttttacaac ggggttgttg
     5821 tggatatact tatcgctaag cagttgttgt gaagccggtg cctccgagaa ttcctgctta
     5881 tcactctctt tactcctata acgaatcgtc attttatcga ccgaagtatc ggttctccac
     5941 tggatcgcct gtgtagtagc cgggtcatca caccaggtca gtacaatctg gtccgcttca
     6001 ggtgatgctg gataggctgt ctcccggaaa gaa
//
LOCUS       DAOSDR010000190         6012 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrimonas sp. isolate AMR_MDS_1826
            NODE_4725_length_6012_cov_3.148733, whole genome shotgun sequence.
ACCESSION   DAOSDR010000190 DAOSDR010000000
VERSION     DAOSDR010000190.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863355
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrimonas sp. (wastewater metagenome)
  ORGANISM  Petrimonas sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Dysgonomonadaceae; Petrimonas.
REFERENCE   1  (bases 1 to 6012)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6012)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:10:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,720
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 35
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6012
                     /organism="Petrimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4725_length_6012_cov_3.148733"
                     /isolate="AMR_MDS_1826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2023866"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..453)
                     /locus_tag="PLI30_08300"
     CDS             complement(<1..453)
                     /locus_tag="PLI30_08300"
                     /inference="COORDINATES: protein motif:HMM:NF012584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo acid dehydrogenase subunit E2"
                     /protein_id="HOI79589.1"
                     /translation="MAQFNIVLPKLGESIQEATVTKWFVKEGDTIHEDDILFEVATDK
                     VDSEIPSPVDGTIIKIFHPENTLLPVGEILAIVKLEDEDENPETSDSTSTTSESNIPE
                     LTKDRGQKGDSGVDDIRSLSNRFYSPLVKKIAQEENLSVSELENIPGTG"
     gene            complement(684..1595)
                     /locus_tag="PLI30_08305"
     CDS             complement(684..1595)
                     /locus_tag="PLI30_08305"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005681149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HOI79590.1"
                     /translation="MVNLAVKIKDLELKNPVMTASGTSGFGEELADFYDVDKLGAILL
                     KGTTLRNRDGNPYPRMAETASGMLNAVGLQNKGIDYFIENIYPRIKDYNTPLIVNVNG
                     STIEEYIEVTQRVNELDKIPAIELNISCPNVKEGGMLFGTSCASAETVTREVRKAYSK
                     TLIVKLSPNVTDIAEIARAVESEGADSVSLVNTFLGMAIDAEKRIPLLSTITGGLSGP
                     AIKPIALRMVWQVSRAVKIPVIGMGGIMNATDAIEFFLAGATAIQVGTAIFRDPFTPL
                     QIIDGLEDYLNRHQIDDICKLVGALKY"
     gene            complement(1589..2359)
                     /locus_tag="PLI30_08310"
     CDS             complement(1589..2359)
                     /locus_tag="PLI30_08310"
                     /inference="COORDINATES: protein motif:HMM:NF012402.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HOI79591.1"
                     /translation="MKKNVGEFTIVSNSALNNTNYHICLQSDIGLPDIAPGQFTNVDI
                     PNNKDIFLRRPFSFFDVDYEKKTVSLLVKILGRGSKSLTELKVGSKLNLVYPLGRGFT
                     IPDKNDKVLAVGGGSGVAPILQLAKTAGSSPGQMHIAIGARSAEDHISIADYLQFAQF
                     YFTTEDGSLGEKGFVTDHPIFHDLTQYNRIYACGPLPMMKAIAARAKEAEIFCEVSLE
                     NLMACGFGVCLCCIEPTVKGNLCVCTDGPVFNIKDLKW"
     gene            complement(2478..2840)
                     /locus_tag="PLI30_08315"
     CDS             complement(2478..2840)
                     /locus_tag="PLI30_08315"
                     /inference="COORDINATES: protein motif:HMM:NF013540.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HOI79592.1"
                     /translation="MNVKSLTAAELADKIGVQRSKVSHVLNGRNNPSSVFIEKLLQTF
                     PELDARWLILGEGEMLMRQEPQQEMKFENNITEMPQKSMTDLLTKSENKIETDSPKNI
                     DRIVILYDDKTFRDYFPE"
     gene            3465..4658
                     /locus_tag="PLI30_08320"
     CDS             3465..4658
                     /locus_tag="PLI30_08320"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HOI79593.1"
                     /translation="MRSIPFLIAIFFLAFYGTITVLAWKSISPLTHTLAFYKRVKRVY
                     WFYSALVWITFMLLFIFPFSIATSKNYSSYYYFNALLFIDILCKAALSISSLIHFFTT
                     NKSRKKIISAMGMLIAAGLFLTFIWGIVWGANTVRKEQVTLHVSNLPASFDGLKIVHI
                     ADIHLGSFHFKHLLEKVNEVSETFQPDLFLFTGDLVNNFSEETEGWQAHFQTFEARLG
                     KFSILGNHDYGDYFRWKSEVERKSNFEKIVEAHSQLGFKLLRNESIPIISGNDTLYLV
                     GVENWGHPPFPQYANLEKAEKSLPKGSFRILLSHDPAHWEAKIKKNENYQLTLSGHSH
                     GLQWGIKPAGIEFSMSYFTRKNWGGLYENDHRYLYVNRGLGTIGIPWRIDMPAEITLI
                     TLRRF"
     gene            complement(4645..>6012)
                     /locus_tag="PLI30_08325"
     CDS             complement(4645..>6012)
                     /locus_tag="PLI30_08325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI79594.1"
                     /translation="ELYVYNLVTTDVYRVGKARFGFDSPALNSDGTQIAISDFTSEGF
                     RLGILSESTLSADYLHDIFRNEYRLAEALTTQEKGIVDFSTADTAKYNSKKYHKLLHL
                     FNFHSWAPLFFDINSYEVKPGFSMMSQNKLGTAEMMLGYKCNLSEKAGQFYTRFEYRG
                     WFPVLSAELTTGKRASSYYLIQTYKNSNGQIIRQDTTLKRFAWDETNLSLNVRIPLNF
                     SQGKYYRLMQPELKYDLTFYKHRPSTPENFPSGSMQTLSYRLYYHQLQKQSYRDLMPN
                     WGWIVDLSLRHSPAVRDSIGNLAAFQVKSYWPGLLPNHGFSVYGGFQQRERGEKYSFG
                     DVVRMPRGEFSIMTNQLASCAFDYKFPLFYPDWNLGRWIYLRRVKASLFYDYAWMQGD
                     IRRNGKISGSFNRNVSTLGTELLADANFLRFYAPANIGFRTIYLANQKQFAVEFLFSV
                     DFKTF"
BASE COUNT         1780 a         1289 c         1102 g         1841 t
ORIGIN      
        1 gcctgtgccc ggaatatttt ctagttccga aaccgatagg ttctcttcct gggcaatttt
       61 tttcaccaat ggagagtaaa aacgattgga gaggctgcga atatcatcaa cgccagagtc
      121 gcctttttga ccgcgatcct tggtcagctc cggaatattc gattctgacg tggttgatgt
      181 agagtcggaa gtttccggat tttcatcctc gtcttccagt ttcacaatgg ccagaatctc
      241 cccaacaggg agcagcgtat tttcggggtg aaaaatttta ataatagtac cgtccaccgg
      301 cgatggaatt tcagaatcga ctttgtcggt agcaacttca aacaaaatat cgtcttcatg
      361 aatcgtatct ccctctttga caaaccattt cgtaacggtt gcctcctgga tgctttcacc
      421 taacttaggt aaaacaatat taaattgcgc catgatacat tgggttttta taattttgca
      481 acatctgcct taaacaacaa gacaaaaatt gaaatgttca ctttgcaata tcaaaaaaac
      541 aataaatcaa tacaggaata gaacaactct gctcctgaca aaattgcagc acgcaaccac
      601 tacgggtgga ttacaaaagt aatttaaagt aaacacaaaa ttatacgcat gaatctcgcc
      661 tgatacaaat gtgaattata gaattaatac ttcaatgcgc cgactagttt acaaatatca
      721 tctatctgat ggcgatttaa atagtcttcc agcccatcaa taatctgtaa aggagtaaac
      781 ggatcccgga atattgccgt tccgacctga atagcggttg cgcctgccaa aaagaattca
      841 atggcatcgg ttgcattcat gataccgccc ataccaatta ccggaatttt gactgcccgg
      901 gagacctgcc aaaccattct gagggcaatg ggtttaattg ccgggccgga aagtccgcct
      961 gtaatcgtag ataaaagcgg aatacgtttc tcagcatcga tcgccatccc caaaaaggta
     1021 ttaaccagcg aaacactgtc agccccttct gattcaaccg cccgtgcgat ctcagcaata
     1081 tccgtaacat taggagacaa cttgactatc agcgttttac tataggcttt gcgtacttcg
     1141 cgcgtaactg tttcagcact ggcgcagcta gtaccgaaaa gcattcctcc ctctttaaca
     1201 ttcggacaag aaatatttaa ctcaatagca ggtattttat ccaattcatt tacccgttga
     1261 gttacttcga tatattcttc aatagttgaa ccatttacgt ttacaataag cggtgtattg
     1321 taatccttta tgcggggata aatgttttca atgaagtaat caatcccttt attctgtaaa
     1381 ccaacggcat taagcattcc tgaagccgtt tcggccattc gcggataggg atttccgtct
     1441 ctgtttcgca atgtggtgcc tttcaggaga attgccccaa gtttgtcaac atcatagaaa
     1501 tcagctaatt cctcaccaaa accagatgtt ccggaagctg tcatgaccgg attcttcagc
     1561 tccaggtctt taatttttac tgctaaattc accatttcaa atccttaata ttaaataccg
     1621 gaccatcggt acagacacac agattacctt taactgttgg ttcaatacag cagaggcaaa
     1681 caccgaaacc acaagccatt aggttctcca acgatacctc acagaagatc tcagcctctt
     1741 ttgctctggc agcaattgct ttcatcatcg gcagtggacc acaggcataa atcctgttat
     1801 attgggttaa atcatggaag atgggatgat ctgtaacgaa tcccttttct cccaaagaac
     1861 catcttctgt tgtgaaataa aattgtgcaa attgcagata atcagctatc gagatatggt
     1921 cttccgctga tcgtgctcct atggctatgt gcatttgccc cggagatgaa ccagcagttt
     1981 tggccagttg caaaatcggt gcaacaccgc ttcctccacc tactgccagc accttgtcat
     2041 ttttatcggg aattgtaaac cctctcccca aaggatatac taaattaagt ttggaaccga
     2101 cttttaattc ggtcaaagat tttgaccctc tacccaaaat tttgaccagt agagagactg
     2161 tttttttctc gtaatccaca tcaaagaagg agaagggtct gcgtagaaag atatctttat
     2221 tattgggtat atctacattt gtaaactgac ctggagctat gtcaggcaga cctatgtctg
     2281 actgtaaaca aatgtgataa tttgtattat taagtgcaga gttgcttaca atcgtaaatt
     2341 caccaacatt tttcttcatt cgtatgcgat ttgagggcaa agatagcgaa gattttaaaa
     2401 aacaaaaaag gccctcatca ggaccttttt ctaattggtt ttgtgcaaag aaattttaag
     2461 ggactactaa aaaactatca ttctggaaaa taatctctaa atgttttatc atcatacaaa
     2521 ataacaatgc gatcaatatt cttcggagag tctgtttcta tcttattttc agatttggtg
     2581 agcagatctg tcattgattt ctgcggcatt tctgtgatat tattttcaaa cttcatctcc
     2641 tgctgaggtt cttgcctcat tagcatctct ccttccccca aaatgagcca cctggcatct
     2701 aactccggaa aggtttgaag caacttttca ataaaaacag aacttggatt gtttcttccg
     2761 ttcaagacgt gtgaaacttt tgaacgctgc accccgatct tgtcagccaa ttctgcagcg
     2821 gtaagtgatt taacgttcat aaatttttta atcctgtctt tcatactacc gtacaaatgt
     2881 aaatactatt tttcaatccg caatagcaaa ataacaattg tatataattt atacgtattc
     2941 taaataaata gcctcaaata gagctctaca tgcccttaaa ttacaaaaca ctgataaaca
     3001 gctgtttatt ttcattgcat tactacatca attactttaa ataaaataca gttttatatt
     3061 gattatcaac aacttattcg taaaaaacaa ttgattttag aataactgct taccttttga
     3121 tctatttcta tttatttaaa ctttaaataa aatgcgctaa tataatgata tagtgtgttt
     3181 tatgctttat tttttttctt tgaaatttta tacaacatca aaatgcattt gatgttaatc
     3241 tttttccttc cataaaaagt atagaggtaa tttttagttg actttttaca ataacgttct
     3301 tacgttgaat tacaaaagac attttacgaa ataaatccca catcattctg atggttcaca
     3361 aaagtaaatc atcgaaattg cctttgacag aaaactttta atctgttctt ttccttttat
     3421 atttgagctt tctatgtatt gatatttatg gtagactaaa aaaaatgaga agtatacctt
     3481 ttttgattgc aatttttttt ctggcttttt atggcactat cactgtcctt gcctggaaaa
     3541 gtatctcacc cctaacccat acactagctt tttataaaag agtcaaacgg gtttattggt
     3601 tttactctgc attggtatgg atcactttta tgctgctgtt tatttttcct ttcagtattg
     3661 caacttccaa aaattattct tcctattatt attttaatgc cctcctgttc attgatatat
     3721 tgtgcaaggc tgctttatcc ataagttcac tgattcactt tttcacaacg aataaaagtc
     3781 gtaaaaaaat catttctgca atgggaatgc tgattgctgc gggattgttt ctgactttta
     3841 tctggggaat agtttggggg gcaaatacag ttcgaaaaga gcaagttaca cttcatgtct
     3901 ccaatttacc ggcttctttt gatggattaa agattgtgca tatcgctgat attcatttgg
     3961 gaagctttca ctttaaacac ctgcttgaaa aagtaaacga agtttcagag acctttcaac
     4021 cagatctgtt tcttttcacc ggcgatctgg tcaacaattt ttccgaagaa actgaaggct
     4081 ggcaagctca ttttcaaact tttgaagccc ggttggggaa attctccatt ctcggcaacc
     4141 atgactatgg agactatttt agatggaaga gcgaagtgga acggaaatct aattttgaaa
     4201 aaattgtaga ggctcattcg caacttggtt ttaaactact aagaaacgaa tcgataccga
     4261 ttatttcagg taacgatacc ctctacctgg taggcgttga aaattgggga catccgccat
     4321 ttccgcaata tgccaatctg gaaaaagctg aaaagagcct tccgaaagga tcattcagga
     4381 ttctgctctc ccatgatccg gcacactggg aagccaaaat caagaaaaat gaaaactacc
     4441 agcttaccct ttcaggacat tcacacggat tgcaatgggg cattaaacct gctggaattg
     4501 aatttagtat gagctatttt acacgcaaaa actggggcgg actgtatgag aatgaccatc
     4561 gctacctgta tgttaacaga ggactgggga cgatcgggat tccctggcgg attgatatgc
     4621 cggctgaaat tacccttatc actctcagaa ggttttaaag tcaaccgaga aaagaaactc
     4681 aactgcaaat tgtttctggt ttgccagata aattgtccgg aacccgatat tagccggagc
     4741 gtaaaaacgt aaaaaattag catccgccaa caactcggtt cccagagtgc ttacatttct
     4801 gttaaaactg ccgctaatct ttccatttct gcggatatcc ccttgcatcc aggcataatc
     4861 ataaaataat gaagccttta cccttcggag gtaaatccat cgtcccaggt tccaatcggg
     4921 ataaaacagc ggaaatttat aatcaaacgc acagcttgcc aactgattgg tcattatgga
     4981 aaattcgcca cggggcatac gtacgacatc accaaaacta tatttttcgc ctctttcccg
     5041 ctgctgaaag cctccgtaga cgctgaaacc atgattgggt aacagccccg gccaataact
     5101 tttaacctga aaagcggcca aatttccgat actatcccgc acggccggcg aatgccttaa
     5161 actcagatca actatccatc cccaattggg cattaaatcc cgatatgatt gcttttgcag
     5221 ctgatggtag tataaacggt aactaagggt ctgcatactg ccagaaggga aattttcggg
     5281 agttgacggc cggtgtttgt agaacgtcaa atcatacttc aactcgggtt gcatcaaccg
     5341 gtaatatttc ccctgactga agtttagcgg tattcgcaca tttaagctca agttggtttc
     5401 atcccatgca aaccgtttta aagtagtgtc ttgcctgata atctgcccat tagaattctt
     5461 gtacgtctgg atcagataat aacttgacgc ccttttgccg gtggttaatt cagcagaaag
     5521 gaccggaaac cacccccgat attcaaaacg ggtataaaac tgacctgctt tttccgataa
     5581 gttacatttg taacctaaca tcatttctgc tgttcccagt ttattttgtg acatcatgct
     5641 gaaacccggc ttgacttcgt acgaattgat atcgaagaac aggggtgccc aactgtgaaa
     5701 gttaaacaga tgtaacaact tatggtattt tttggagtta tacttggcgg tatcggctgt
     5761 tgagaagtca acaatacctt tttcctgggt tgtcaatgct tcagccagcc gatattcatt
     5821 tcggaatata tcgtgcaggt aatcagcgga caatgtgctt tcagagagga ttcccagccg
     5881 gaaaccttcg gaggtaaagt cactgatggc aatctgggtt ccatcgctat tcagcgccgg
     5941 gctgtcaaat ccaaaccgtg cctttcccac cctgtaaaca tctgtcgtaa ccaggttgta
     6001 aacatacagc tc
//
LOCUS       DAOSDR010000191         6009 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrimonas sp. isolate AMR_MDS_1826
            NODE_4729_length_6009_cov_3.166947, whole genome shotgun sequence.
ACCESSION   DAOSDR010000191 DAOSDR010000000
VERSION     DAOSDR010000191.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863355
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrimonas sp. (wastewater metagenome)
  ORGANISM  Petrimonas sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Dysgonomonadaceae; Petrimonas.
REFERENCE   1  (bases 1 to 6009)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6009)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:10:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,720
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 35
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6009
                     /organism="Petrimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4729_length_6009_cov_3.166947"
                     /isolate="AMR_MDS_1826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2023866"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..598)
                     /locus_tag="PLI30_08330"
     CDS             complement(<1..598)
                     /locus_tag="PLI30_08330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI79595.1"
                     /translation="MGLRIKIICVITVGILASLHANAFPNFITRDGDQLKDGKNVFRF
                     ISVNTPNINGHYDGYKNTNPESGYIYDPIELSFEMEAYFKDMVQMGATVIRSWGITVE
                     DGSGEFEALVTGPHSYNETAFRRIDKMLELCNRYNIRVILCLVKENKFWGSTASFSKL
                     YGGGNYYEDPVVKEGFKRLLKTVATRRNYFTGKEYKDDK"
     gene            complement(649..2040)
                     /locus_tag="PLI30_08335"
     CDS             complement(649..2040)
                     /locus_tag="PLI30_08335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008565624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endo-1,4-beta-xylanase"
                     /protein_id="HOI79596.1"
                     /translation="MNWINTGLSRKKRMMFGMVVFTITIFNGNPLKSQITNSKLSGAT
                     NTMSIAYWEYWNPDVRKKIDEDIEKNRKSDAVYKIKNVKVGTEVQVTQISHDFLFGGN
                     IFLFGDLGTPEKNKRYEDTFGRLFNAATVPFYWKTLEPEQGKPRYQAGSSYEYRRPAT
                     DPVVEFCESKGINMNGHTIIYGMRRWGHPSWMPEDRKKMEEYFENHIRELAERYKGRI
                     QRWDVVNEPTDQANRGIMPDDYTYKCYLWAMKYFPDSVQLNINDSDMHWDMTLFRRYL
                     EIVRNMIDREIRMDNIGMQMHIFNPEESRKVANGADILTPEKIYERLDLMADAGRPLH
                     VSEVTISAPDNTDEGKTIQAEIAKNLYRLWFSHPKVMGITWWNVVDGGAAPGEPSISG
                     LFDPDLNPKPVYQVLDNLINYEWKTNLTVRTDRNGIIKFRGFKGRYQLEWTDNSGQEQ
                     RAEFDLKSDGDGI"
     gene            complement(2048..4849)
                     /locus_tag="PLI30_08340"
     CDS             complement(2048..4849)
                     /locus_tag="PLI30_08340"
                     /inference="COORDINATES: protein motif:HMM:NF014850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HOI79597.1"
                     /translation="MKLRIKNHLVFVLVFLAFASRSQENLLLSPYYIFPRNSSQHIDL
                     SNDWQLSSRDLPVHDINELDLNSWINVVYPTSVQMAHFKAGILGDPYKNLNAREHEKL
                     EQKVWYYKKEFTVPVSSQNNNILLNFDGIDYFAKVWLNGQLLGSHEGIFGGPIIDITK
                     RIEFGKKNELIVEVASANYGKPNFDPWIPGKIVKGWFLTGGSAMEPYFNLGLWRNVRI
                     EILPKYHIERPFLSTKSIFNDNAVLELSLEIFSGKNSLDYELHPKGNVQISNFSSPKS
                     AVQNKKVDDKISVTIELKYDGKVAFSKEFFPYVIEGRCWLEEKFNVTNPKLWYPNGLG
                     EQDYYTASVTLNVNGKPADNIEFDFGIRTIEYERSAGIRTSDRWNNWQFIVNGKKIFI
                     KGINWMPVDALYDLTEDKYDWAVKMAKNAGIQMFRIWGSGLLESKEFYEACNKYGIMV
                     WQDFNIANFDTPEWPQDVWEAQVCQNIFRLRNHPSLAVWCGGNEFNPYSYGNAATMGI
                     LERNIKIFDPTRSFLRTSPDEGSIHSYPDFDPAWYKGFELVPFVAETGIHCITDPKTI
                     REVVDESELSDLRGMYETTFRDSHPEFVEHFAEYNPYRVPRMLSRASHIADLSNPTLE
                     TLAEATQIGAGEFYQVMSEGFQMNYPVTTGLMPWVYKRPWPVVAAINLVDGNGQPSAP
                     YYFLKRTYEPVHVALDIQRLLWAPGETFPLKVKVINGINQPSSNAKVVVEVFDNQFKS
                     VQMEEKQIHIQEGTSVSGINFSEFKIPESYKEKYFFIQVRLLDIDNRLISSAEYWPRT
                     IRQMEEPEYYHLFNNEPVPWPTLDKGPWLKPTVALTKTTLDVKLITSKSDASNSCQID
                     LKITNTGKIPAFMVNMDITGIKRAFYATENYYWLAPGECKDITINVLWREPTNNKKVT
                     MEIGAWNTKKNIVKLDNLK"
     gene            complement(4861..5778)
                     /locus_tag="PLI30_08345"
     CDS             complement(4861..5778)
                     /locus_tag="PLI30_08345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI79598.1"
                     /translation="MFTQKLAFYSKYYKDNPAIFGWELWNEINAVHIPDKPESVLKWT
                     NTMLPLSKTYFPNHLVMQSLGSFDRKSSFNLYEQFVTLLSNEIAQVHRYLDPGASWEI
                     CQAPMDILASNAVRELQNMVENKPIVLSEVGAVEANHSGPSLLYQKDNLGVLLHDMLF
                     APFFSGAAGPGQSWHWQFYIEKNNLWWHFGRFEKAIRGINPIIENFIPQFCEIDGVRL
                     YVLKGKSNILIWGRDSKSDWVTEIVEGKNPEKRNLNIPLSKLLISDKQYNVKMYDPWN
                     DLWSKIKIENNGLTIPQFERSFVIRIENE"
BASE COUNT         1720 a         1321 c         1033 g         1935 t
ORIGIN      
        1 ccttgtcatc cttatattcc tttcccgtga aataatttct ccgggttgcc acagtcttga
       61 gcaggcgttt aaaaccttct tttaccaccg ggtcttcgta ataatttcct cctccataca
      121 atttagaaaa tgaagcagtt gaaccccaaa atttattttc ctttaccagg cacagaatta
      181 ccctgatgtt gtacctgttg cacaattcca gcattttatc gatgcgcctg aaagcggttt
      241 cgttataaga atgaggtcct gtaaccaggg cttcaaactc gcccgaacca tcttctacgg
      301 taattcccca ggagcggata acagtagctc ccatttggac catatctttg aaataagctt
      361 ccatttcaaa ggaaagttct attggatcgt agatgtaacc gctttcagga ttggtgtttt
      421 tataaccatc gtaatgcccg ttaatattgg gtgtattgac actgataaag cggaatacgt
      481 ttttaccgtc ttttagttgg tccccgtcac gggtaataaa attggggaat gcatttgcat
      541 gcaaagaggc aagaattcca accgttatta cgcaaataat tttaattctt agtcccatca
      601 ctttcagcat caataattaa tcatgtactc tatgcagaca gatatgattt atattccatc
      661 tccatcgctt ttcaaatcaa attctgctct ttgttcctgg cctgagttat ctgtccattc
      721 taactgataa cgacctttaa atcctcggaa tttaataata ccatttctat ctgtccggac
      781 tgtcagattt gtcttccatt cgtaattgat caaattatca agaacctgat atactggttt
      841 aggatttaag tccggatcaa ataatccgga aatggatggt tcacccggag cggcacctcc
      901 gtcaactacg ttccaccagg tgattcccat tacttttggg tgactaaacc acaatcggta
      961 aagattcttt gcaatttctg cctgaatcgt tttgccttca tctgtgttgt caggtgcact
     1021 aatcgtaacc tcgctcacat gcagcggtct cccggcatct gccattaaat ccagcctttc
     1081 ataaattttt tcaggggtta aaatatcagc tccatttgca actttacggg attcttcagg
     1141 attaaagatg tgcatttgca tgcctatatt atccattcga atttcccgat caatcatgtt
     1201 tcgtacgatt tcgagatacc ggcgaaaaag agtcatatcc caatgcatat ctgaatcatt
     1261 gatattaagc tgcaccgaat ccggaaaata tttcatcgcc cataaatagc atttataggt
     1321 ataatcatcg ggcataatcc cacggtttgc ctgatctgta ggttcgttca ccacatccca
     1381 tcgttgaatc ctccccttgt aacgctctgc cagttcgcga atatggtttt caaaatattc
     1441 ttccattttt ttgcgatctt cgggcatcca cgatggatgt ccccagcggc gcataccata
     1501 aataatggta tgtccgttca tgttaattcc tttcgactca caaaattcta ccacaggatc
     1561 ggtagccgga cgacggtatt catacgaact gccggcttgg tatctcggct tgccttgttc
     1621 gggttcaagt gttttccagt aaaatggaac cgtcgcggca ttaaacaaac gaccgaaagt
     1681 atcttcataa cgcttgtttt tctccggcgt accaaggtca ccgaataaaa agatattacc
     1741 accaaacaaa aaatcatgac tgatttgagt tacctgaacc tcagttccaa cctttacatt
     1801 tttaatcttg taaaccgcat ccgattttct attcttttcg atatcctcat caattttttt
     1861 ccggacatcc ggattccaat actcccaata agcaatactc attgtgttag ttgcacccga
     1921 taacttacta tttgttattt gtgattttaa tggatttcca ttgaagatag ttatagtaaa
     1981 caccaccatt ccaaacatca ttcttttctt ccgggataat ccagtattta tccaattcat
     2041 tctaattcta ttttaagtta tccagtttaa caatattttt tttggtattc caggcaccaa
     2101 tttccattgt gactttctta ttgttagttg gttcacgcca cagtacatta atggtaatat
     2161 ctttacattc acccggagca agccagtaat aattttccgt tgcataaaac gcgcgtttta
     2221 taccggttat atccatattc accataaacg caggtatctt accggtattg gtaattttta
     2281 aatcaatctg acagctattg gaagcatcag atttactggt aattaatttt acatccagcg
     2341 ttgttttagt aagggctacc gtcggtttta accaaggacc tttatccaaa gtcggccacg
     2401 gaacgggttc attattaaac agatgatagt attcaggctc ctccatctgc cgaatggtac
     2461 gtggccagta ttcggcgctt gaaatcaacc ggttgtctat atccagtaat ctgacttgaa
     2521 taaagaaata tttttcttta taagattctg gaattttaaa ctcagaaaaa tttatacctg
     2581 aaacagacgt tccttcctga atgtgtatct gtttttcttc catctgaaca gatttgaact
     2641 gattatcaaa aacctcaacc acaacctttg catttgatga cggctgattt atcccgttga
     2701 ttacttttac cttaagagga aatgtttcgc cgggagccca caatagtctt tgaatatcga
     2761 gagcaacatg cacgggttca taagtccgtt tcagaaaata atagggagca gaaggctgtc
     2821 cgttaccatc aaccaaattt atggcagcca ccacgggcca gggcctctta taaacccagg
     2881 gcatcaatcc tgtcgtgacc ggatagttca tttgaaatcc ttcggacata acctggtaga
     2941 attcccctgc accgatttga gttgcttcgg caagagtttc aagagtagga ttgctcaaat
     3001 cagcgatatg agaagccctg ctcaacattc tgggtacacg gtaaggatta tattcagcaa
     3061 aatgttcaac aaactcgggg tgggaatccc gaaatgttgt ttcatacatt cctctcaaat
     3121 ccgacaattc actttcatca actacctcac gtattgtttt agggtctgtt atgcagtgga
     3181 ttccagtctc ggctacaaac gggacaagtt caaatccctt ataccaggca ggatcaaaat
     3241 ccggataaga gtggatggat ccttcatcag gagaagtccg gagaaaactc cgggtaggat
     3301 caaatatttt gatattgcgt tcaagaatcc ccattgtggc agcgtttcca tatgaatacg
     3361 gattaaattc gtttccacca caccaaactg caagcgacgg atgattacgc agccggaaaa
     3421 tattctggca gacctgtgct tcccaaacat cctgtggcca ttcaggggta tcgaagtttg
     3481 cgatattaaa atcctgccac accataattc catatttgtt gcaggcttca taaaattcct
     3541 tgctttccag caatccgcta ccccaaatac gaaacatttg aattcctgca tttttggcca
     3601 tcttcactgc ccagtcatat ttgtcttctg ttaaatcgta cagggcatct accggcatcc
     3661 agtttattcc ttttataaat atcttttttc cattgacaat aaattgccaa ttgttccaac
     3721 gatcacttgt ccgaatccca gcacttcttt catattcaat ggtgcggata ccgaaatcaa
     3781 attcgatgtt gtctgccggt tttccattaa cattaagtgt aactgatgct gtgtaatagt
     3841 cctgttcgcc aagaccattg ggataccata attttggatt ggttacatta aatttttctt
     3901 ccagccagca cctgccttca attacgtaag gaaaaaattc ttttgaaaag gcaactttac
     3961 cgtcgtattt taattcgatg gtaacactta tcttgtcgtc aactttttta ttttgtactg
     4021 cacttttggg tgagctgaaa tttgaaatct gaacgttccc ttttggatgt aattcataat
     4081 ccaacgaatt tttcccggaa aaaatttcca gcgacaattc gagaactgca ttatcattaa
     4141 aaatactttt ggtagaaaga aacggacgtt caatatgata ttttggcaag atttcgatac
     4201 gcacattacg ccataaccct aaattgaaat agggctccat tgcactacct ccggttaaaa
     4261 accagccttt aacaattttc ccgggaatcc acgggtcgaa atttggtttt ccataattgg
     4321 cagaagcaac ttcaacaatc aattcattct ttttaccaaa ttcgattctt ttagtaatat
     4381 caataattgg cccgccaaag attccttcat gacttcccaa caattgtccg tttagccaaa
     4441 cttttgcaaa ataatcaatg ccatcaaaat tcagcaaaat gttattattc tgtgatgaaa
     4501 cgggaacagt gaactccttt ttataatacc atactttttg ttcgagtttt tcgtgttcgc
     4561 gggcattcag atttttgtat gggtctccta aaatgcccgc cttaaaatga gccatctgca
     4621 ctgatgtggg gtacactacg ttaatccacg aatttaaatc caattcgtta atatcatgaa
     4681 caggtaaatc tcttgaactt aactgccagt catttgacaa atcgatatgt tgtgaactat
     4741 tacgtggaaa aatgtaatag ggcgacaaaa gcagattttc ctgcgaacgg cttgcaaacg
     4801 caagaaaaac aagtacaaaa actaaatgat ttttaatcct taacttcata acaaactatt
     4861 ttattcattt tcaatcctaa ttacgaaaga tcgttcgaat tgtggtatgg ttaatccatt
     4921 attctcaatc ttaatttttg accacaaatc attccaggga tcgtacattt ttacattata
     4981 ttgtttatca ctgattagca atttggaaag cggaatattt aaatttcgtt tttccggatt
     5041 tttgccttcc actatctctg ttacccagtc tgacttactg tccctgcccc atatcagaat
     5101 gttacttttc ccttttaaca cataaaggcg aacaccatca atttcacaaa actgggggat
     5161 aaaattttct attatcggat taattccacg aattgccttt tcaaaacggc caaagtgcca
     5221 ccacaaattg tttttttcaa tataaaactg ccagtgccaa ctttgtccag gcccagccgc
     5281 acctgaaaaa aacggggcaa aaagcatgtc gtgtaacaat actcccaaat tgtctttttg
     5341 atataacaag gaagggcctg aatgattagc ctcaacagca cccacttccg ataacacaat
     5401 tggtttattt tcaaccatat tttgtagctc cctaactgca ttacttgcca gaatatccat
     5461 aggagcctgg catatttccc aggaagctcc gggatcgaga taacgatgta cctgtgctat
     5521 ttcatttgac agaagagtaa caaactgctc gtaaagatta aagctgcttt tccggtcaaa
     5581 acttccgaga ctttgcatta caaggtgatt tgggaaatag gtttttgaaa ggggcaacat
     5641 agtattagtc catttcaaaa cagactcagg tttatccgga atatgcacag catttatttc
     5701 attccatagt tcccatccaa atattgccgg attatcttta taatatttgg aataaaaagc
     5761 caacttctgg gtaaacaatt cctttccttc gggtgtatta aaatagtctt ctgtcgattc
     5821 cagcggacct ccgtattttt ttgaatagat aggtttatca aatggtaccg accccgggaa
     5881 aattgccgga taatcttcga gagccctgaa attttccaaa cagaatttta ttttaatatc
     5941 atatttttcg gctaatgcta aaatcttatc aattcgtttt ttaacgtttt ggtcgtatga
     6001 tgctgcttt
//
LOCUS       DAOSDR010000192         5984 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrimonas sp. isolate AMR_MDS_1826
            NODE_4754_length_5984_cov_4.436667, whole genome shotgun sequence.
ACCESSION   DAOSDR010000192 DAOSDR010000000
VERSION     DAOSDR010000192.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863355
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrimonas sp. (wastewater metagenome)
  ORGANISM  Petrimonas sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Dysgonomonadaceae; Petrimonas.
REFERENCE   1  (bases 1 to 5984)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5984)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:10:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,720
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 35
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5984
                     /organism="Petrimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4754_length_5984_cov_4.436667"
                     /isolate="AMR_MDS_1826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2023866"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..159
                     /locus_tag="PLI30_08350"
     CDS             <1..159
                     /locus_tag="PLI30_08350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006744540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4301 family protein"
                     /protein_id="HOI79599.1"
                     /translation="QKSKNGKELKALELPGLWNGAMSDWNTIFVEVPVSTFNPVKTVN
                     DLLRLEHQ"
     gene            complement(200..3115)
                     /locus_tag="PLI30_08355"
     CDS             complement(200..3115)
                     /locus_tag="PLI30_08355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009859611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HOI79600.1"
                     /translation="MKKITSFAIIVLIATISTSVFSQNSDLKYKTVPNDPISARIYTL
                     DNGLKVYMSVNPETPRIQTYIAVRVGGKNDPAETTGLAHYFEHLMFKGTKKFGTQDYT
                     QEEPMLKEIEGLFEKYRNTEDDTQRRAIYKEIDSISYAASKLAIPNEYDKLMKTIGAT
                     GTNAYTGFDMTVYTENIPSNQIENWARIQSDRFQNNVIRGFHTELETVYEEKNMSLTR
                     DSRKVYEKVLASLFPHHPYGKQTVLGTQEHLKNPSIINIKNYYKTYYVPNNMAICVAG
                     DFDPDEMIKIIDTYFGEMKPNPSLPVIEPSAGQPITKPVETDVLGQEAENVTLGWRMG
                     GWATPDKDMLDIISLIMNNGKAGLIDLNLLQQQKVLSAYSGAYSMADHCAFVMNATPK
                     KGQTLEQVRDLLLGEVEKLKKGDFPQELITASINNMKLSEMYRIESNGGRANWFVNSF
                     INGTEWANEVTRIDRIAKITKKQIVDFANEKFRNNYAVIYKREGKDPTELKIDKPQIT
                     PIATNRDAVSTFVKEIQDARVTPIEPVFLDFDKDMKILTAKSKIPVLYKQNVTNGIFS
                     LIYVFDMGNNHDKALGTAFDYLKYLGTSTKSPEEIKANFYSLACSFNVFPGTERVYVL
                     LDGLAENMSKALALFEELLADPQVNKEAFANLSADILKKRGDAKLNQGANFSKLTQYA
                     SWGADSPDNNILSEAELKSMDPQQLTTRIKSLNGFEHRIMYYGPENEKELLSTINSLH
                     NVPAKLKPVPETDRFKQVETNENKVLLAEYDAKQIYLGMVSNDGRLFDPKVEAMRELY
                     NEYFGGSMNAIVFQEMREARGLAYSANAYLNAPTKLKYPYTMRTFIATQNDKMDDALQ
                     AFHLILKEMPLSENAFNLAKEGLITRLCTNRIIKEDVLWNYIYAQDLGLKVDRRKEIF
                     EKVPSMTLSDIKAFQEEWVKKRTYTYYILGKEADLDLKALEKYGPIQKVSQEELFGY"
     gene            3446..>5984
                     /locus_tag="PLI30_08360"
     CDS             3446..>5984
                     /locus_tag="PLI30_08360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006800591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CusA/CzcA family heavy metal efflux RND
                     transporter"
                     /protein_id="HOI79601.1"
                     /translation="MLNKIIRFSVRHKLIIILFTITIIGFGIFALANLSVGAVPDITN
                     NQVQVITTSANLATQDVEQYITMPVELAMANLPGVKEIRSVSKFGLSVVTIVFDEKLG
                     TYLPRQLIAEKISEASENIPQGFGTPVMGPVTTGLGEIYQYILDEKPGYENRYSITDL
                     RTIQDWIVKRQLSGIPGVVEVNTWGGFLKQYEIAVNPELIRSQGITLMEVFDAIDKNN
                     NITGGSYIEKNNQSYFIRGDGAIKSLEDIRSIVVKNRNGIPVLIGDIAVVQFGHANRF
                     GAITANGEGEKVMGQIMMLKNADSKKVIKAVHKRVAEVQETLPEGIFINPIVDRSELI
                     GRTTMTVFENLLFGCVIVILVVLLILGNLRTAMIIASIIPLSLLFTLSLMYIFGIDAN
                     LMSLGALDFGIIIDGGVIIAEFIALSLNNHYDDANRLHGQERKDYIDEITINGASKMM
                     NSAIFGQIIILIVFVPIISLTGVEGKMFRPMALTFCFAVIGASLLGMTWLPVASALFM
                     KPGKNGEKNVSRVMRLAYKTYRPSIEWACGHKRMIVIGALVMLTVALLLFGKLGAEFT
                     PTLDEGDFVIQPVLKTGTSLRKTIEMTTQMEAILKKEFVEVDQVVSRIGAAEVPTDPM
                     SMEEIDMIIKLRPKKEWKNAGSKEELADKFKEALSVFPGIEYEFTQPIEMRFNELVTG
                     VRSDIAVKIFGEDLDYLAEKAVEIAAQARMVEGAADVIVEKTVGLPQMRVTYNREKLA
                     YYGVSIESLNQYLSMAFGGQISGTVFERERRFDLVVRFLEDFRQDIDNIRQMYVNLPD
                     GNQIRLSELAQIEYSSGPAKISRDNTRRRVVVSVNVRNRDLQSVVQDI"
BASE COUNT         1502 a         1241 c         1442 g         1799 t
ORIGIN      
        1 caaaaatcca aaaacggaaa agaactgaag gccctggagc ttcctggctt gtggaacggt
       61 gccatgagcg actggaacac cattttcgta gaggtgcccg tcagtacctt taatccggta
      121 aaaacggtga acgatttgct acggttggaa catcagtaaa accaattagg gcgccatttg
      181 gcgccctaaa tgctttatcc taatacccga acagctcttc ctgagaaacc ttttggatag
      241 gcccgtattt ctcgagcgct ttcaggtcta aatccgcttc tttacccaat atataatagg
      301 tgtacgtgcg tttttttacc cattcttcct ggaatgcctt tatgtcggac agtgtcatag
      361 acggtacttt ttcgaaaatt tcttttcgcc tgtccacttt caagcccagg tcctgtgcat
      421 aaatgtagtt ccacagcaca tcttccttga taatcctgtt ggtgcacaaa cgggtaatca
      481 agccttcttt ggccaggtta aacgcatttt ccgataacgg catctctttc agaataaggt
      541 ggaacgcctg tagtgcatcg tccattttat cgttttgagt agcaataaac gttcgcatcg
      601 tgtaggggta tttcaatttc gtcggagcat tcaggtacgc atttgccgaa taagccagtc
      661 cgcgcgcttc gcgcatttcc tgaaacacga tcgcattcat gcttccgcca aaatattcgt
      721 tgtataattc acgcatagct tcgactttcg gatcaaacag ccggccgtcg ttggaaacca
      781 ttcccaggta aatctgtttt gcatcgtatt cggctaaaag cactttgttc tcgttggttt
      841 ctacttgttt gaaccggtcg gtttcgggta ccggttttaa ctttgccggg acattgtgta
      901 acgagttaat tgtggatagc agttcttttt cgttttcagg accataatac ataatccggt
      961 gctcaaaacc gttgaggctt ttgatgcgtg tggtcagttg ctgcggatcc atcgatttca
     1021 attctgcttc cgacaatata ttgttatcgg gagagtccgc tccccaggac gcatactggg
     1081 ttaatttgct gaaattagcc ccttggttga gtttagcatc gccgcgtttc ttcaggatat
     1141 ctgccgacag gttggcgaat gcttctttat ttacctgagg gtcggccagc aactcttcga
     1201 aaagcgcaag agccttgctc atgttttctg ccaggccatc gagcaaaaca tatacccttt
     1261 ctgtccccgg aaaaacgttg aacgagcaag ccagactgta aaaattggct ttgatctctt
     1321 ccggtgattt tgtggatgtg cccaggtatt ttaaataatc gaaagccgta cccagggctt
     1381 tatcgtggtt atttcccatg tcgaacacat aaatcaggct gaaaatgccg ttggttacat
     1441 tctgcttgta gagcacgggg atctttgatt tggcagtcag tattttcatg tccttatcga
     1501 agtcgagaaa cacaggttca atgggtgtga ccctagcgtc ctgaatttct tttacgaatg
     1561 tgcttaccgc atcacgattg gtggcaatgg gcgtgatttg tggtttgtcg atctttaact
     1621 ccgtgggatc ttttccttcg cgtttataga ttacggcata attgttgcgg aatttttcgt
     1681 tggcaaaatc cacgatctgt tttttagtga tttttgcgat acgatcgatc cgggttactt
     1741 cgttggccca ctccgtcccg ttgatgaatg agtttacaaa ccagttagcc cgtcctccat
     1801 tgctctctat tcgatacatc tcgctcagct tcatgttgtt gatagatgca gttatcagtt
     1861 cctggggaaa atcacctttt ttcagttttt ccacttctcc cagcaacaaa tctcttacct
     1921 gctcaagtgt ctgtcctttc ttgggcgtgg cattcataac aaaagcgcag tggtctgcca
     1981 tgctgtaggc acccgaatat gcagaaagca ctttttgctg ctgcagcagg ttcaggtcga
     2041 ttaatccggc ttttccgtta ttcataatta ggctgatgat atcgagcata tctttgtcgg
     2101 gtgtggccca tccacccatt cgccagccga gggtcacgtt ttcggcttcc tgccccagca
     2161 catccgtttc aaccggcttt gttataggtt gcccggcaga tggttctata actggcaggc
     2221 tgggattagg tttcatctct ccgaaatagg tatcgataat cttgatcatt tcatcaggat
     2281 caaaatctcc ggcaacgcag atagccatgt tgttcggaac gtagtaggtc ttgtagtagt
     2341 ttttaatgtt tatgattgac ggattcttca ggtgttcttg tgtgccgagc acggtctgtt
     2401 taccgtacgg gtggtgaggg aacagtgagg caagcacttt ttcgtacact ttccggctgt
     2461 cgcgggtgag tgacatgttt ttctcctcat aaaccgtttc cagttctgtg tgaaaaccac
     2521 gaatcacatt gttttgaaaa cggtccgatt gtattcttgc ccaattttcg atttgattcg
     2581 acggtatatt ttccgtataa accgtcatgt caaaacccgt ataggcgttt gtccctgttg
     2641 ccccgatagt cttcatcagt ttgtcgtact cgttggggat ggctagcttg gatgcggcgt
     2701 aggagatact gtcaatttcc ttgtaaatgg ctcttctttg ggtatcgtct tctgtattcc
     2761 ggtatttctc gaagaggcct tcaatctctt ttagcatcgg ctcttcctgc gtgtaatcct
     2821 gggtgccgaa tttttttgta cccttaaaca tcaggtgttc gaaataatga gcaaggccgg
     2881 tggtttctgc cggatcgttt ttccctccca ctcgtacggc aatataggtt tgtatacggg
     2941 gggtttcagg attcacgctc atgtaaactt tcaacccgtt atccagggtg taaatacgtg
     3001 cactgatcgg atcattggga actgtcttat acttcagatc actgttctga gagaatacgc
     3061 tcgtggaaat agtagcgatt aagacaataa ttgcgaatga ggtaattttt ttcataggtt
     3121 acaatttaaa aatgagtacg ttgtggtttt tcccttttgg gaaagctacg atgatgtcgt
     3181 acaaagatag tgttattttg ctgcaatcac caaataaaac ttgaattttt attgttcagt
     3241 gagtagggag aaaactttaa aggtttatgc tacgtctgaa ttttaagccg tttatttttt
     3301 gatagataag tggcttttgc gtgacttgct tatttagaac aagaaaaaat taaaatactg
     3361 gataaataag atttgattta tcttgcattt ttttgttcat ttgtgtagct ttcagaacat
     3421 tatatgaaaa ctttttcatg cgtttatgtt aaacaagata attcggttta gtgtcaggca
     3481 caagctgata atcatacttt tcacaattac gattattggt ttcggaatat ttgcattagc
     3541 caatctttcc gtaggcgcgg ttcctgacat taccaacaat caggtacagg taattaccac
     3601 atcagccaac cttgcgacgc aagatgtgga gcaatacatc actatgcctg tggagttagc
     3661 catggcaaac ctgccgggtg tgaaggagat acgttccgta tcgaaatttg gattatcggt
     3721 agtcaccatt gttttcgatg aaaagttagg tacttaccta ccgagacaac tgattgccga
     3781 aaaaatatcg gaagcgtctg aaaatattcc ccagggcttt ggtacacctg ttatggggcc
     3841 ggttactact ggcctgggcg agatttacca atacattctc gatgaaaaac cgggttatga
     3901 aaaccgttat tccataaccg acctgcggac cattcaggac tggattgtga aaagacaact
     3961 gtcgggaatt cccggagtag tggaagtgaa tacatgggga ggatttctca aacaatacga
     4021 gattgccgtt aatccggagt tgatacgtag tcagggaatt acgttgatgg aggtctttga
     4081 tgccatcgat aagaacaaca acataaccgg cggctcatac atcgaaaaaa acaaccaaag
     4141 ttattttata cgtggcgatg gagctataaa atcgctggaa gacatccgaa gtattgtggt
     4201 gaagaacaga aacggaatac cggtactgat cggcgatatt gccgtagtgc agttcggaca
     4261 tgcgaaccgt tttggagcca ttactgccaa cggcgagggc gaaaaggtta tgggccagat
     4321 tatgatgctc aaaaacgccg attcaaaaaa agtaattaaa gctgtacaca aaagggtggc
     4381 agaagtacaa gaaactcttc cggaagggat ttttatcaat cccattgttg atcggagcga
     4441 gcttatcgga cgcactacga tgaccgtctt cgaaaacctg ctgttcggat gcgttattgt
     4501 aatcctggtg gttttgctta tcctgggaaa ccttcgtact gcgatgatta tcgcctccat
     4561 catccccttg tcgttacttt ttacgttgtc gttgatgtac attttcggta ttgatgctaa
     4621 cctgatgagc ctgggcgcac tcgacttcgg gattattatt gacggtgggg tgatcatagc
     4681 cgagtttatt gcgcttagtc tcaataacca ctatgatgat gcaaataggc tgcacgggca
     4741 ggaaagaaaa gactacatcg atgagattac gatcaatggc gcatccaaaa tgatgaactc
     4801 cgctattttc ggacaaatca ttatcctgat tgtttttgtg ccgattattt cgttaacggg
     4861 agtggaaggg aaaatgttcc gtccgatggc actcacgttt tgttttgccg ttattggggc
     4921 ttctctattg gggatgacat ggctgcctgt ggcatcagcc ctgtttatga aaccggggaa
     4981 aaacggagaa aagaatgtct cccgggtaat gcggctggct tacaaaacat acagaccgtc
     5041 cattgaatgg gcgtgtggac ataaaaggat gatcgttatc ggtgctttgg ttatgctgac
     5101 ggttgccttg cttctttttg gtaagcttgg ggcagaattt acgcctacgc ttgatgaagg
     5161 tgactttgtg attcagccgg tgctgaaaac agggacgtca ttgaggaaaa ccatcgagat
     5221 gactacgcaa atggaggcaa tattaaaaaa ggagtttgtg gaagtcgacc aggtggtgag
     5281 ccgtataggt gctgcagagg ttcccaccga tccgatgtcg atggaagaga ttgacatgat
     5341 catcaagctc cgtccgaaga aagagtggaa gaacgccggg agtaaagaag agcttgccga
     5401 taagtttaaa gaggcccttt cggtttttcc gggtatcgaa tacgagttta cacaacccat
     5461 agagatgaga ttcaatgagt tggttactgg agtgcgctca gatattgctg tgaaaatttt
     5521 cggtgaagat ttggattacc tggcggaaaa ggccgtggag attgcagcac aggcccgtat
     5581 ggtagaagga gctgccgatg ttattgttga gaaaacggta ggcttaccac aaatgagggt
     5641 aacgtacaat cgcgaaaaac tggcgtacta cggcgtttcc atagaatcgc tcaaccaata
     5701 cctgtcgatg gctttcggcg gacaaatttc ggggacggtt ttcgagcggg agcgcagatt
     5761 tgatctggtt gttcgttttt tggaagattt ccgccaggat attgataaca taaggcagat
     5821 gtatgtgaat cttcccgatg gaaaccagat acggttaagt gagttggctc aaattgaata
     5881 ctcttccggt ccagcaaaaa tttcgcgtga caatacccgg cggcgggtgg tggtgagtgt
     5941 gaatgtgcga aaccgtgact tgcaatcggt ggtgcaggat attc
//
LOCUS       DAOSDR010000193         5948 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrimonas sp. isolate AMR_MDS_1826
            NODE_4791_length_5948_cov_4.331410, whole genome shotgun sequence.
ACCESSION   DAOSDR010000193 DAOSDR010000000
VERSION     DAOSDR010000193.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863355
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrimonas sp. (wastewater metagenome)
  ORGANISM  Petrimonas sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Dysgonomonadaceae; Petrimonas.
REFERENCE   1  (bases 1 to 5948)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5948)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:10:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,720
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 35
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5948
                     /organism="Petrimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4791_length_5948_cov_4.331410"
                     /isolate="AMR_MDS_1826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2023866"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..290
                     /locus_tag="PLI30_08365"
     CDS             <1..290
                     /locus_tag="PLI30_08365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI79602.1"
                     /translation="VLERDGKLTDQEHAAFVTLLKKSGIEFMKESYGFDSEENIGCSV
                     IYMLSLTPEGGKTRYINIRENAEDRFSDAFREMILYSSLFLNNKLETDSAY"
     gene            674..1501
                     /locus_tag="PLI30_08370"
     CDS             674..1501
                     /locus_tag="PLI30_08370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI79603.1"
                     /translation="MKKNERAPSVYRLAHLWPLAVIIIGIGACAAGAAVSQPYWDFFG
                     LLLILSLAALLGGALFGFLFGVPRLNRNYDPREDYGRTTKYMPNTNLEEVSDWLTKII
                     IGVTLTQLTKIPGYLQGMADYIVANSDCSTLDCNFAGPVIISLFIYFFIAGFISGYYY
                     TRIFLPNLFSVMEENSILKAETAIWREGGKKMFSLAGEPEVSHKIEYFTDKEREMLQK
                     IKVQNNVFADIHKLSHQEYAVLNVLIAKGIVEIYPGNLSTGKETLHITDEEVLQSLQ"
     gene            1577..2833
                     /locus_tag="PLI30_08375"
     CDS             1577..2833
                     /locus_tag="PLI30_08375"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HOI79604.1"
                     /translation="MKPPVLGKKLLRDYPAAESSSGVLPREALANDTEENPLYTGRYL
                     LILRDGNKDVVKTTKLLETKWGFLVANTADFVSEPMCESRIKDADALFYNELGIALVA
                     VDDDKMKLLHTAQADYVVEPEKVVYIPDDVPVDTAVDSTWGIRAVKAHESAYTGAGIK
                     VAVLDTGFDMKHPDFKGRSLTAFSFVPDEAVDDLHGHGTHCIGIVCGSSGLRDMRYGV
                     APEAQIYAGKVLNNGGRGAQAWILDGMTWAANNGCRVLSMSLGSKVLPGQSYDIAYER
                     AAQFALSKGTLIVAAAGNDSRRSENQFSPVSSPADCPSILAVGAVNSTLQVADFSNRA
                     LNESGLVDLAAPGVGIYSSWPMPARYRTLSGTSMATPHVAGVVALLCEKFPDATPAMI
                     GQELIRTAGRLSSPAVDIGAGISLAP"
     gene            2830..3060
                     /locus_tag="PLI30_08380"
     CDS             2830..3060
                     /locus_tag="PLI30_08380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI79605.1"
                     /translation="MKQIIITISAYYLDRLDVVAENLREEGVTITRLYEFGVIIGYAE
                     EEIIDKIRHHKEIASLTDEKDVVIPPPEEDVQ"
     gene            complement(3063..3584)
                     /locus_tag="PLI30_08385"
     CDS             complement(3063..3584)
                     /locus_tag="PLI30_08385"
                     /inference="COORDINATES: protein motif:HMM:NF013098.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4260 family protein"
                     /protein_id="HOI79606.1"
                     /translation="MSPYTIPTQVRIGHVHLKVSDLERALSFYRDLLGFEVTMMYGRQ
                     AAFLSAGGYHHHIGLNTWHSEGMPEAPLEGVGLYHTAIAYPTRHDLALILKRLIEAEY
                     PLTGASDHGVSEAIYLNDPDGNGVELYWDKPKAEWQMEADGSLNRILGYGLKYSDSFG
                     NTHLGKICKKDVV"
     gene            complement(3593..4021)
                     /locus_tag="PLI30_08390"
     CDS             complement(3593..4021)
                     /locus_tag="PLI30_08390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019540452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6157 family protein"
                     /protein_id="HOI79607.1"
                     /translation="MHTTNYFDTFIEVASDTRMTQGTPPPLKGKKTVARLQYEFISEN
                     PYQYSSDDILFHVYAEKNDTPSDKLEQTRILFFSKGQPCLRTSPLTKIYGFGIHCNSE
                     GKVALYGMETEGYRIFLADSKIKKVKAMRSGREQQTKSNQ"
     gene            4287..4724
                     /locus_tag="PLI30_08395"
     CDS             4287..4724
                     /locus_tag="PLI30_08395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019538554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI79608.1"
                     /translation="MQDQRRDIKTDSVIKNPAYDADLAEKLGADAYGMKNFVLVILKT
                     GSNVTDDKAFITECFRGHMDNINRLEKEGKLVVAGPMAKNEQHYRGIFILDIATIEEA
                     EALLGSDPAVREKLLAADLYKWYGSAALPLYLEHANKIWKKQP"
     gene            4693..5166
                     /locus_tag="PLI30_08400"
     CDS             4693..5166
                     /locus_tag="PLI30_08400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019538553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HOI79609.1"
                     /translation="MRTRYGRNNPDQFITMGELKTKQHDGDVTEFIRSYTDSEQKRAD
                     SFALVKLMREVTGFEPKMWGDTMIGFGQYHYKSERSRQEGDWMLVGFSPRKTAISLYL
                     YSGILEQEELLAGLGNFKMGKGCIYVKKLSDIDPEVLRKIIFSTVAYLKETYGKL"
     gene            5363..5899
                     /locus_tag="PLI30_08405"
     CDS             5363..5899
                     /locus_tag="PLI30_08405"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOI79610.1"
                     /translation="MTIEVDSEIELKQLEPSDAKDIFETIDLQRDYLGRWLPFVAQTK
                     DISDTEKFVDSVVNASEDQFEYVFTIRKYNQFIGLIGFKDTDKQNKKTEIGYWLSEKH
                     QKQGIVTKSVDKLCDFAFDKLELNRIQIKCAVQNRSSSNIPKRLGFRLEGVERQGEWL
                     SDGFYTDLEIYSKLKSDN"
BASE COUNT         1623 a         1228 c         1544 g         1553 t
ORIGIN      
        1 ctgtattaga gcgtgatgga aaactcactg accaggagca tgctgctttt gtgacacttc
       61 tgaagaagag tggaatagaa tttatgaaag agtcgtatgg tttcgacagt gaagaaaata
      121 ttggatgctc ggtcatttac atgctgtcgc taacaccaga gggaggtaag acgcgctata
      181 tcaacatccg ggaaaatgca gaggatagat tttcagatgc gttcagagag atgatcctgt
      241 attcaagttt gtttctaaat aataaactag aaactgattc tgcttactaa aaaaaacctc
      301 ttcgtgggca tgactagtct taaagaaccg ttaccggttt ataagacaaa aatagtttat
      361 tattcggcag gacggtcgac cttcctgccg gatttttttt atggcgttct tattttatgt
      421 aacgctatct caggaagaga tagttcgtga ccacctttcc agcggataca ataaaatttg
      481 ccgctgaaac ttggatatct caaaagattc cattaatttg cactttcgtt gtgatgtaat
      541 ctgctactca ggattttctg gcctgtgtgg tcccattgag cctgttcctg caggattgag
      601 gtttacctgt gtccaagaca ggtatttacg cctgtcagag gtgagaaatt gaaaaaacct
      661 tgcttaaaaa acaatgaaaa aaaatgaacg cgcacccagc gtgtaccggc ttgctcacct
      721 gtggcccctc gcagtcatca ttatcggaat aggggcatgc gctgccggtg cggccgtttc
      781 tcaaccttac tgggatttct tcggattgct tcttatcctc tctctggcag cgttgcttgg
      841 cggagccctg tttggttttc tttttggagt gccgcgattg aaccggaact atgatccgcg
      901 ggaagattat ggacgtacca caaagtacat gcccaataca aatctcgagg aggtgtcgga
      961 ctggctgaca aaaattatta tcggggttac gctgacacaa ctgacaaaga ttcccggtta
     1021 cctgcagggc atggcggact atattgttgc aaatagcgac tgcagtaccc tggattgtaa
     1081 ctttgccggg ccggtgatca tctcgctctt tatctatttt tttatagccg gttttatatc
     1141 cggttactac tacacccgga tattcctgcc taacctgttt tccgttatgg aggaaaatag
     1201 catccttaaa gccgagactg ccatctggag agagggtggc aaaaaaatgt tctccctggc
     1261 cggtgagccg gaggtctccc ataagattga gtacttcacc gacaaggaga gagagatgct
     1321 gcaaaagatt aaagtgcaaa ataacgtgtt tgccgacatt cataagctga gccatcagga
     1381 atatgctgtc ctgaacgtgt tgattgcaaa gggaatagtt gagatctatc ccggtaacct
     1441 ttcaacaggg aaggaaacgt tgcacatcac tgacgaagag gtattgcaat cgctgcaata
     1501 aatagagacc ggggatcgaa agccagatgg cgcgagccct aaacaaattg tacaaaacca
     1561 cttaaattaa aaagcaatga aaccgcccgt tttaggaaaa aaactcttga gggattatcc
     1621 cgcagcggag agctcctccg gtgtattgcc ccgtgaagct cttgccaatg acacggaaga
     1681 gaatccgctt tatacaggaa gatacctgct cattctcagg gatgggaata aagacgtggt
     1741 aaaaaccacc aaactgctcg aaacgaaatg ggggtttttg gttgccaata cggctgattt
     1801 tgtgtcggaa cccatgtgcg aaagtaggat aaaggatgcg gatgcattgt tttacaatga
     1861 attgggtatt gcccttgtgg cggtagacga tgataaaatg aaactgttgc ataccgcaca
     1921 agccgattat gttgtagaac cggaaaaagt agtttatatt cccgacgatg ttccggttga
     1981 cacagccgta gactccacct gggggattag ggctgtaaag gcgcatgagt cggcatatac
     2041 cggggcaggg ataaaagtcg ctgtgctgga taccggtttc gacatgaagc atccagactt
     2101 taaagggaga agtcttacgg ccttttcatt tgttcccgat gaagctgtgg atgacctcca
     2161 cgggcatgga acgcattgta tcgggattgt ttgcggttcg tcgggtttac gggatatgcg
     2221 gtacggtgtg gctccggaag ctcagattta tgcgggaaaa gtgttgaata atgggggaag
     2281 aggagcgcag gcctggattc tggacggaat gacctgggct gcgaataacg ggtgtcgagt
     2341 gttgtccatg tcgctgggtt ctaaagtcct tccgggacag agttatgaca tcgcctatga
     2401 aagagctgcc cagtttgctc tttcgaaagg gacactcatt gtggcggcag cagggaatga
     2461 cagcagacga tctgaaaacc aattcagtcc ggtgagcagt ccggccgact gcccttccat
     2521 cctggctgta ggtgcagtaa actcaacttt gcaggtagcc gatttctcaa accgggcact
     2581 taatgagagc gggttggtgg atctggcggc acccggagtc gggatctatt cttcctggcc
     2641 catgcccgcg agataccgta ccctttccgg gactagcatg gctacaccgc atgtggcggg
     2701 agtggtcgcc ctgctgtgcg aaaagtttcc cgatgccacc cctgcaatga tcggacaaga
     2761 gctgatccgg acggcaggcc gtttgtcgtc tcccgctgta gatataggcg caggaatttc
     2821 tttggcacca tgaagcagat tattatcacc atatccgctt actacctcga ccggttagac
     2881 gttgtggcgg aaaacctgcg ggaagaaggg gtgacgatta cccggctcta cgagtttgga
     2941 gtgatcatcg ggtacgccga agaagaaatt atcgataaaa tccgtcatca caaagagatt
     3001 gcctctttga cggacgaaaa ggatgttgtt atccctccac cggaggagga tgttcaataa
     3061 ggctacacaa cgtctttttt acagatttta cccaggtgtg tgttgccgaa gctgtcgcta
     3121 tatttcaacc cgtatcccag tatccggttg agggaaccgt cggcttccat ctgccattct
     3181 gctttcggct tatcccagta gagttctaca ccgttgccgt cgggatcgtt caggtagatg
     3241 gcttccgata ccccatggtc gcttgcgccg gtaagagggt attccgcttc tatcagccgt
     3301 ttcaggatta gggccaggtc gtgacgggta gggtaggcaa tagcagtatg gtagagaccc
     3361 actccctcca gcggtgcttc gggcatcccc tcgctgtgcc aggtgttcag gccaatatgg
     3421 tggtggtatc caccggcaga gaggaaggcc gcctgcctgc cgtacatcat ggtgacctcg
     3481 aagcccagaa ggtcgcgata gaaagaaagg gcgcgctcca ggtcggacac cttcaggtgt
     3541 acatgtccaa tgcgtacttg tgtagggatt gtataaggac tcataatttt ttttattgat
     3601 tactttttgt ttgttgttct cttcctgacc gcattgcttt cactttcttg attttagaat
     3661 cggccagaaa gattcgatat ccctccgttt ccatgccgta aagggcaact ttcccctcac
     3721 tgttgcaatg aatgccgaaa ccatagatct ttgtcagggg cgatgtccgg agacagggtt
     3781 gccctttgga aaagaacagg attctagtct gttccagctt atcggagggt gtatcgttct
     3841 tttccgcgta cacatggaag aggatgtcat ccgaactata ttggtaggga ttctcggaaa
     3901 taaattcgta ttgtagacgg gctaccgtct ttttcccttt gagcggaggg ggagttccct
     3961 gtgtcattct tgtgtcggat gcaacctcaa taaacgtatc aaaataattg gttgtatgca
     4021 tgttattcaa cagatgatgg tgttaccgga cagctgacta ggaccggctt ccagggatac
     4081 ctgttacgta accaataaca gatttcatgt gcagattgtt ttgtgttttt taagaacaaa
     4141 ttatatccta gtgcgttaaa ttgctgaaaa ctgaccggca agagtgtaca gctctttata
     4201 aagcatattg tatgaaaatt attttatcag tttctatctt gtttgttaca tttgtagttt
     4261 ctgcttttcc ttcatcagat cctattatgc aggaccaaag gagggatata aaaacagatt
     4321 cggttataaa aaaccctgct tatgatgctg atctagcgga gaagctggga gccgatgctt
     4381 acgggatgaa gaacttcgtg ctggtaatcc tgaagacagg gtcaaatgtg acggacgaca
     4441 aggcatttat aactgaatgt tttcgtgggc acatggacaa tatcaaccgc ctggagaaag
     4501 aggggaaact ggttgtggcg ggtccgatgg caaagaacga gcagcactat cgaggtattt
     4561 ttatcctcga tatagcaaca atcgaagaag ctgaggcatt gttggggagc gatccggccg
     4621 tgagggagaa attactggct gccgatctct ataaatggta cggctctgcc gccctaccgc
     4681 tttatctgga gcatgcgaac aagatatgga agaaacaacc ctgatcaatt catcacgatg
     4741 ggagaactga aaacaaaaca acacgacggt gatgtgacgg aatttatccg cagctatacc
     4801 gacagtgaac aaaaacgtgc cgacagcttt gcattggtga agctgatgcg cgaggtgacc
     4861 ggtttcgaac cgaagatgtg gggagatacg atgatcggat tcgggcagta ccactacaag
     4921 tcggaacgca gccggcagga aggtgactgg atgctggtgg gtttttcgcc ccgtaagact
     4981 gccatatcgc tctacctcta ctccggtatc ctggaacagg aagagctgct cgccggcctg
     5041 ggaaatttca aaatggggaa aggatgtatt tacgtgaaga agttatcgga tatagacccc
     5101 gaagtgctgc gaaagatcat cttctccacg gtagcctacc taaaggaaac ctacggcaaa
     5161 ctataacatt acactgcatg ggaaagggaa ttgattacag tggacagtgg aaggaggaac
     5221 tggctctgcg cttatatccg tgagacggtg aaaaacgaac aggagggagg tacccatgat
     5281 catggaagga agaggcattg atgccggtgt tttttaatgt tgatttgtgg gagataaaat
     5341 atatttgaaa gaagaaaatg atatgacaat agaagtagat tcagaaatag aattaaaaca
     5401 actggagcca tcggacgcaa aagatatttt tgaaacgatt gatcttcaaa gagactacct
     5461 ggggagatgg ttaccgtttg ttgcgcagac gaaagacatt tcagatacgg aaaaatttgt
     5521 tgactcagta gtaaatgctt cagaggacca atttgaatat gtctttacca tcaggaagta
     5581 caatcaattt attggtctga ttggttttaa agacaccgac aaacaaaata aaaagacgga
     5641 aatcgggtat tggctttccg aaaaacacca gaaacagggg attgttacaa aatcggtcga
     5701 taaactttgt gactttgctt ttgacaaatt ggaattaaac aggattcaga taaaatgcgc
     5761 agtacagaac agatcgagca gtaatatacc gaagaggttg ggattcaggc ttgaaggagt
     5821 tgaacgacag ggggaatggc tttctgacgg tttttatact gacctggaaa tttacagtaa
     5881 attgaaatcg gataattaac aggatgaggc aaaccaaaaa ggagttgaag tttgttattt
     5941 ttttcgca
//
LOCUS       DAOSDR010000194         5936 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrimonas sp. isolate AMR_MDS_1826
            NODE_4803_length_5936_cov_4.194355, whole genome shotgun sequence.
ACCESSION   DAOSDR010000194 DAOSDR010000000
VERSION     DAOSDR010000194.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863355
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrimonas sp. (wastewater metagenome)
  ORGANISM  Petrimonas sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Dysgonomonadaceae; Petrimonas.
REFERENCE   1  (bases 1 to 5936)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5936)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:10:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,720
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 35
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5936
                     /organism="Petrimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4803_length_5936_cov_4.194355"
                     /isolate="AMR_MDS_1826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2023866"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..385)
                     /locus_tag="PLI30_08410"
     CDS             complement(<1..385)
                     /locus_tag="PLI30_08410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019541239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HOI79611.1"
                     /translation="MKNLETTFMGIKLDNPVILGASNMSSHLDQLKKAEQQGIGAVVY
                     KTLFEEQVQLENLQLDERLSQYAHIHAEMTSIHPDVNFSDIDYHLSRLRKAKESLSVP
                     VFASLNAVNRESWISYARMLEQTGID"
     gene            744..1613
                     /locus_tag="PLI30_08415"
     CDS             744..1613
                     /locus_tag="PLI30_08415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019540109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HOI79612.1"
                     /translation="MKVVTGLVLLICCSSVYGQKNSIISKNAKIEKVGTGYSFTEGPA
                     VSGEGRVYFTDQPNDRIYVWDEGKGISLWAEETGRSNGLYVDADGQLVSCADLHNQIV
                     RFGKDKKMQVVYEKYDGKQLNGPNDLWITPKGGIYFTDPYYHRNYWREGHAEVQDVRG
                     VYYLSPDGEVIRVIADYKQPNGLVGTPDEKTLYVADINDRKIWKYDIQPDGTLANKTF
                     FAPHGSDGMTMDNKGNVYLTMGKVWVYSPKGELIQEIEVPESPSNVCFGGKKRNILFI
                     TARTSVYTIKIKL"
     gene            1626..2621
                     /locus_tag="PLI30_08420"
     CDS             1626..2621
                     /locus_tag="PLI30_08420"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOI79613.1"
                     /translation="MNTVLVGFGFMGMTHTLNILKNPRIHLKAIVDKVPGNIFTKLTE
                     QSGNFATGNVDAKDLSGVNIYSDFAECLKVEKPDACVIAVHTALHYEMAEMALQSGAN
                     VFLEKPFCIDAGEGERLINLARSKNKVLMVGHVVRFMPAYETLKKWIDSGEYGRLEFL
                     ALSRFSGVPIWGQWKERQKEFGSSGGALFDLVIHDIDFAQWVCGVPDQIEARCLPGKL
                     STHDYVSALWKYNDSDVVVKIDGGNTFHASYPFHAGFSARFENASVFFSSQDPHNIQV
                     VTDTETMLFSGGDANDGFSNELYYFAECVLQGAQPVKCTPESALDSIRICHRHTL"
     gene            2658..4013
                     /locus_tag="PLI30_08425"
     CDS             2658..4013
                     /locus_tag="PLI30_08425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019538197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015295 - solute:proton symporter activity
                     [Evidence IEA];
                     GO_process: GO:0015718 - monocarboxylic acid transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate:H+ symporter"
                     /protein_id="HOI79614.1"
                     /translation="MSTLFLTFIVVSAVAILLFLVLKLKVSAFIALLLTSIYVGVLTG
                     MPLSSITKSIQEGMAGTLGFVATVVGLGAIFGQMLESSGGAESLAHHLVKKFGKERAP
                     WAMVTTGFLVAIPIFLDVAFIILVPIVYALSRDTKLSLLYYAIPLLAGLAVTHSFIPP
                     TPGPIAVADIISVPLGWVIFMGFILGIPTAIIAGPIFGKYISKKIYLESPYSSGDVSP
                     EFDPENYPSFRSIAVIISIPLLLILLNTITAVAVSKGVVTKSVFTDIVEFIGHPFSAL
                     IIATLIATYFLCIRRGMDKQKVLQLSTKALGPAGIIILITGAGGVLKQVLIDSGIGQM
                     MAESMANSALPPILLAWILSALVRVTQGSATVAMITAASIIAPIIAEFGLNDPQRALV
                     VISIASGATLLSHVNDSGFWLVGKYLGMNEKQTLQSWTVMETIIAFCGLGFTLLASLF
                     F"
     gene            4116..4475
                     /gene="rsfS"
                     /locus_tag="PLI30_08430"
     CDS             4116..4475
                     /gene="rsfS"
                     /locus_tag="PLI30_08430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005854895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HOI79615.1"
                     /translation="MKENNKLLQSIIEGIQEKKGKNITQIQLKGIPGAICDYFVICEG
                     NTPTQVSALAESVEEVVKRNTWESPIRVQGKQRAEWIGMDYGTIIVHIFVPELRHYYN
                     LDHLWEDASLEQVPALD"
     gene            4534..>5936
                     /gene="ftsH"
                     /locus_tag="PLI30_08435"
     CDS             4534..>5936
                     /gene="ftsH"
                     /locus_tag="PLI30_08435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007749126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HOI79616.1"
                     /translation="MENNNQNQQDNNNPKKPVRPGGLGNTNPNQPFNVYWIYAILSMG
                     LIGMLFFGQNKTIKKVDWSDFQEYVNDNRIESIVVYSNKDIAEAKVRSESVNYVFGEE
                     AGRFGANPFIQVNIPSTQGTSEFLDTVKKEKAYDIKVRFDTTSEIWGMLLTFAPFLLI
                     IVFWIWMMRRMQGGPGGGGGGIFSVGKSKAQLFDKDAGPKVTFKDVAGLSEAKEEVQE
                     IVEFLKHPGNYTNLGGKIPKGALLVGPPGTGKTLLAKAVAGEANVPFFSISGSDFVEM
                     FVGVGASRVRDLFRQAKEKSPCIVFIDEIDAVGRARGKNVNFGSNDERENTLNQLLTE
                     MDGFGSNSGVIILAATNRVDILDKALLRAGRFDRQIYVELPDLNDRKEIFKVHLRPIK
                     IDESVDVEFLARQTPGFSGADIANVCNEAALIAARNKKKFVEREDFISAVDRIIGGLE
                     KKNKIITQDEKKSIAIH"
BASE COUNT         1607 a         1196 c         1445 g         1688 t
ORIGIN      
        1 cgtcaatccc ggtttgttct aacatccggg catagcttat ccacgattct ctattcacag
       61 cattaaggct ggcaaacaca ggtacggata aactttcctt cgctttacga agtcttgaca
      121 agtgataatc gatatcggaa aaatttacat cgggatgaat actggtcatt tctgcgtgaa
      181 tgtgtgcgta ctggcttaac ctttcatcca attgcaggtt ttccaactgt acctgctctt
      241 caaacaaggt tttataaacc acagctccaa tgccttgctg ttcagccttt tttagctggt
      301 cgaggtgtga cgacatattg ctggcgccca atataaccgg attgtccagt tttattccca
      361 taaaggtagt ttcaagattt ttcatagatt ctttaaattt tatgttgtga ttttaggcca
      421 ggcatccact actggtcaag ggactccggt cacctcttaa cctttaatgt ccggctcctg
      481 tccgcatatg aatatataac attttttcgc tcgaatagtt ttgcccttac aatattacgg
      541 tttaatttgc tttttaccgt tacctgagag gggaaatgag cggctagcta caattaaaat
      601 cggggtggat agttgtattt ctgtgcaata taactatatt tgccaaagag tctgttttca
      661 gtcaaactca atcagtttta tatatgattt gcacttctct tcagctccac cggacaacaa
      721 tatgaataat aaaacataaa cggatgaagg ttgtaacagg attggtttta ttgatctgtt
      781 gcagttctgt ttacggacag aaaaacagta tcatttcgaa aaatgcgaaa attgaaaaag
      841 tggggaccgg ttattctttt accgagggtc cggccgtttc gggagagggt agagtctatt
      901 ttactgatca gccgaacgat cgcatttatg tctgggatga aggaaaaggc atttctctgt
      961 gggctgaaga gaccggtaga tccaacggat tgtatgttga tgctgacggt caattggtct
     1021 cctgtgctga tttacacaac cagatcgtgc gtttcgggaa agataaaaaa atgcaggttg
     1081 tatatgaaaa gtacgacgga aaacagttga acggtcccaa cgacttatgg attaccccca
     1141 aagggggcat ctatttcacc gatccttatt atcatcggaa ctattggaga gaagggcatg
     1201 ctgaagttca ggatgtgcgg ggagtctatt acctaagccc cgatggagag gtcatccggg
     1261 ttatcgccga ttataaacaa cccaacggat tagtcggtac gcccgacgaa aaaacgttgt
     1321 atgttgccga tatcaatgac cggaagatat ggaagtacga catccagccg gatggaaccc
     1381 tggccaacaa gacttttttt gcaccccatg gatccgacgg aatgaccatg gacaacaaag
     1441 gaaatgtata cctgaccatg ggaaaagttt gggtatactc ccccaaaggt gaacttatcc
     1501 aggaaataga agttcccgaa agcccttcaa atgtttgttt tggcggaaaa aaaagaaaca
     1561 tcctgtttat caccgcccgg acttcggtat ataccataaa aataaaatta taaataagat
     1621 aatttatgaa tacagtttta gttggtttcg ggttcatggg aatgacccac acgttgaata
     1681 ttttgaaaaa tccgcgtatt catttgaaag ccattgttga taaagttccc gggaacatct
     1741 tcacaaagct tacagaacag tcgggtaatt tcgccaccgg taatgtcgat gcaaaagatc
     1801 tttcgggcgt gaacatttat tctgattttg cagaatgtct gaaagtggag aagcccgatg
     1861 cctgtgttat tgcagttcat acagcattgc attatgagat ggctgaaatg gcgcttcagt
     1921 caggagcaaa tgtctttctt gaaaaacctt tttgcattga tgcaggggag ggggaaaggc
     1981 tgattaacct tgcccgttcc aaaaacaagg tgttgatggt tggtcatgta gtacgtttta
     2041 tgccggctta cgaaacattg aaaaaatgga tagattcggg tgaatacggt cgcctagagt
     2101 tcttagcgtt gtcgcgtttc tcgggtgtgc ctatctgggg gcaatggaaa gagagacaaa
     2161 aagagtttgg atcgtcgggt ggtgctttgt tcgatttggt tattcacgat atcgattttg
     2221 ctcaatgggt ttgcggtgtt cccgatcaga tagaggcccg gtgcttgccg ggaaaattaa
     2281 gcacccatga ctacgtgagc gcattgtgga agtataatga ttcggatgtc gttgtcaaaa
     2341 ttgacggagg caatacgttt catgccagtt atccgtttca tgcggggttt agtgcccggt
     2401 tcgagaatgc cagtgttttc ttttcctcgc aagacccgca caacatacaa gtagttacgg
     2461 acactgaaac tatgctgttt tcaggaggtg atgcaaacga tggtttttcg aacgaacttt
     2521 attatttcgc cgaatgcgtt ttacaaggtg ctcaacctgt caagtgtact ccagaatcgg
     2581 cgttggattc cattcggatt tgccataggc atactcttta aacggcctga aaccagaaat
     2641 cttccatacc ggtaaatatg tcaacactct ttttaacttt tatcgtcgtc tccgcagtag
     2701 caatactgtt attcctggtt ttaaaactca aggtcagtgc gtttatcgct ttgttgctca
     2761 catccatata cgtaggcgtc cttaccggaa tgcctctcag cagtatcacc aagtccattc
     2821 aggaaggaat ggcgggaacg ctcggttttg tggctaccgt tgtaggattg ggggccattt
     2881 tcgggcaaat gctggaaagt tccggaggag cggaatcatt agcacaccac ctggtgaaaa
     2941 agtttggcaa ggaacgggct ccctgggcaa tggtaacaac gggcttcttg gtggctatcc
     3001 ctatttttct ggatgtggcc tttattattc tggttcccat cgtgtatgca ctgtcccggg
     3061 ataccaaact ctcgttattg tattacgcca tcccgcttct ggcaggatta gcggtaacgc
     3121 atagctttat tcccccaacg cccgggccga ttgcggtggc agatatcatc agtgtgccgt
     3181 tgggctgggt aatttttatg ggatttattc tgggtatccc tactgcgata attgcggggc
     3241 caatcttcgg gaagtatatt tctaaaaaga tttacctgga atcgccgtat tcatccggcg
     3301 atgtttcacc ggaattcgat ccggaaaact acccgtcgtt ccggtcgatc gcggtgatta
     3361 tctctattcc gttgctgttg attctactga acacgatcac agcggtggcg gtttctaaag
     3421 gcgttgtcac taaatccgtt tttacagaca ttgttgagtt tattgggcac cctttttcag
     3481 cattgattat tgccacattg attgccacgt actttttatg tatccgaagg ggcatggaca
     3541 agcaaaaggt acttcaatta agcaccaagg cacttggccc tgccggaatc atcattctga
     3601 taaccggtgc cggcggtgtt ctgaaacagg tattgatcga tagtggtatt ggccagatga
     3661 tggcggagtc catggcaaat tctgcactgc cgcctattct actggcatgg attctttcgg
     3721 cgttggtgcg ggtgacgcaa gggtctgcca ctgtagccat gatcactgct gccagcatta
     3781 ttgccccaat tatcgctgag tttggcttga atgatccgca acgggcgttg gttgtgatct
     3841 ccatagcatc gggagctacg ttgctgtcgc atgtcaacga cagtggattc tggttagtag
     3901 gaaaatacct gggaatgaac gagaagcaaa cactccaaag ctggacagta atggagacca
     3961 ttatcgcttt ttgcggattg ggatttacgt tgttggcgag tttgtttttt tgatttattt
     4021 tttggcactc ttcttgctgt tatttttgtg tatgaaagat aaggtgattg tgaaaaagcg
     4081 actatctttg tatacgtaac tcattaaaac agcgaatgaa agaaaataac aagttattac
     4141 aaagcatcat tgaagggata caagagaaaa aagggaagaa catcactcaa attcaattga
     4201 aaggaattcc gggagcaatt tgcgactatt ttgtcatttg tgagggaaat acccctactc
     4261 aggtttctgc attggcagaa tcggtagaag aggtggtgaa aagaaatacc tgggaaagcc
     4321 ctatcagagt ccagggaaaa caacgtgccg aatggatagg tatggattac ggtacaataa
     4381 tcgtacatat ttttgtgccc gaattacggc actattacaa cctcgatcat ctgtgggaag
     4441 acgcttcatt ggagcaagtt ccggcattgg attaaaactt attttcatct gaagtgttac
     4501 atatattcat caaaaatttc ataggagacg ttaatggaaa ataacaacca aaatcaacaa
     4561 gataacaata acccaaaaaa gccggtccgt cccggaggat tgggaaacac aaatcccaat
     4621 caacccttta atgtttactg gatatacgcc attttgtcga tggggcttat cggaatgtta
     4681 tttttcggac aaaacaaaac cataaaaaag gtagattggt cggattttca ggaatacgta
     4741 aacgataacc gtattgaaag catcgttgtc tacagcaata aagacattgc tgaagccaaa
     4801 gtgcgttccg aatccgttaa ttatgttttt ggagaagagg ctggccggtt tggcgccaac
     4861 ccgtttatac aggtaaatat cccctctacc cagggaactt cggagtttct tgataccgtt
     4921 aaaaaagaaa aggcatacga cataaaagtc cgtttcgata ctacgtcaga gatttggggc
     4981 atgttgctaa cctttgcgcc ttttctgctg atcattgttt tttggatatg gatgatgaga
     5041 cgcatgcaag gcggtcccgg aggcggtggc ggtggtatct tcagtgtggg aaaatccaaa
     5101 gcacagcttt tcgataagga tgctgggccc aaggttactt ttaaagatgt ggcagggctt
     5161 tctgaagcca aggaggaggt tcaggaaatt gttgagttcc tcaagcatcc gggtaattac
     5221 accaacctgg gggggaaaat tcctaaaggc gccttgctgg taggccctcc gggaacaggg
     5281 aagacacttc tggcaaaagc ggttgccggc gaagccaacg tgccgttctt ttccatatcc
     5341 ggttccgatt ttgtggaaat gtttgttggg gtaggggcat cccgtgttcg cgacctgttt
     5401 cgtcaggcta aggaaaaatc gccgtgtatt gtgtttattg atgagataga tgctgttggg
     5461 cgtgcccgtg gaaagaacgt caactttgga tcgaacgatg agcgtgaaaa tacgctgaac
     5521 caacttctta cggagatgga cggctttggt tcaaacagcg gagtgatcat tctcgcagct
     5581 accaaccggg tggatatttt ggataaagcc ttacttcggg ccggccgttt cgaccggcag
     5641 atttacgtgg agttgccgga tttgaacgat agaaaagaga ttttcaaggt tcacctgcgc
     5701 cccattaaaa ttgacgaatc ggtggatgtg gagtttctgg cgcgccaaac acccggattc
     5761 tccggtgcgg atattgccaa tgtttgtaac gaagccgctc ttatagctgc ccgaaacaaa
     5821 aagaaatttg ttgaaaggga agactttatc agtgctgttg accggattat tggcggtctg
     5881 gagaagaaaa acaaaatcat cacgcaggat gaaaaaaaat caattgctat ccacga
//
LOCUS       DAOSDR010000195         5910 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrimonas sp. isolate AMR_MDS_1826
            NODE_4830_length_5910_cov_3.874637, whole genome shotgun sequence.
ACCESSION   DAOSDR010000195 DAOSDR010000000
VERSION     DAOSDR010000195.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863355
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrimonas sp. (wastewater metagenome)
  ORGANISM  Petrimonas sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Dysgonomonadaceae; Petrimonas.
REFERENCE   1  (bases 1 to 5910)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5910)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:10:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,720
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 35
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5910
                     /organism="Petrimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4830_length_5910_cov_3.874637"
                     /isolate="AMR_MDS_1826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2023866"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1155
                     /locus_tag="PLI30_08440"
     CDS             <1..1155
                     /locus_tag="PLI30_08440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019538130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI79617.1"
                     /translation="TYAGHFLVEAKNKGFDVSQSVIGRWVGFQQKLARNWTRIDSHRG
                     YYGISMTELQQAYRLYALALSGNTELGAMNRMREIADLNLQAKWRLAAAYALAGKPDV
                     ANSLVFNASDAVEDYRSNNDTYGSPARDKAMIMQTYLLLGNIEKALQLAPDMSRALSS
                     DYISTQTVAFRLMAMAQLAEKMGSGNIDVDWTLNGKKMAAVNTPHAFHQVDLKTAPNQ
                     SVQISNKGKGKVYARLTAFMQPLVDTLRAAEGSLRLSVNYLDAAGKPLDVKSLKQGTE
                     FTAVVTVRNSVEQSFTDLALLQVFPSGWEIFNERLTGTQSAAETYNYRDIRDDRVLTY
                     FNLGAGQSATFRARLQAAYRGNYYLPAVSCQAMYEPREQARTSGIWVEVR"
     gene            1137..3479
                     /gene="pbpC"
                     /locus_tag="PLI30_08445"
     CDS             1137..3479
                     /gene="pbpC"
                     /locus_tag="PLI30_08445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005923928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 1C"
                     /protein_id="HOI79618.1"
                     /translation="MGRGKMIDKFLSLKRWEKATLIIGFILMMGYAFSLPDPLFDASY
                     STLVSDRNGELLGARIASDGQWRFPPTDSIPDKYKTCLMAFEDRYFRYHPGVNPLSLG
                     RAMVQNIKAGRVVSGGSTLTMQTVRLMRKNKRTYFEKFIEIILATRLELSYSKKRIIA
                     LYASHAPMGGNVVGIDAAAWRYFGHGARSLSWAESATLAVLPNSPSLMHFGRNRDALL
                     TKRNRLLQKLLDNGTISETDYRLATAEPLPENPHSLPQIAPHLVTRLYLSQPGTHVQS
                     TIDKSLQLQADNVLERWNTEFSQNHILNLAALIVDLEKNEVISYIGNVNFGKQQSGNQ
                     VDIIQSSRSTGSILKPFLYCAMLQEGALLPGELLPDIPINVGGFSPKNFSLQYDGAVH
                     ANEALARSLNVPSVVSLRRYGVPKFYDFLKKAGLTTLNRPADYYGLSLILGGAEGTLW
                     EVANAYADMAHAVRTVAPRKGEFSLVHEGQDYRGGRVNAFTDAGAAWLTLEALTNVNR
                     PEEIDWQFIPSMRRVAWKTGTSFGFRDAWAVGVTPKYLVAVWTGNASGEGRPGLTGAR
                     TSARVMFDLFNLLPPTSWFETPYNHLTEVVVCRESGCLVGLHCPPSSADTVFAPAKAV
                     QGSVCNYHQRVHISEDERYRVYEHCAVGRGIRPVSWFVLPPSWEWYYKQQHPTYRSLP
                     PFSPECSDGASGEVMQFIYPYPNSVIKITRQLDGSRGKAVFELAHRNPSARVFWHLDN
                     EYLGETADVHQMELSPESGAHGLTVVDEMGNSLTIRFKVE"
     gene            complement(3512..4636)
                     /locus_tag="PLI30_08450"
     CDS             complement(3512..4636)
                     /locus_tag="PLI30_08450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018463520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOI79619.1"
                     /translation="MTNPFLIKGYLSKEFFCNRVEELKELERNVKNGIDTTLISARRL
                     GKTGLIMRLFEGLKGEPFELIYVDIYASRTQSDFIKLLIEAILKKIPPKTTIGKNFFS
                     LLKSLRPVISYDPISGEPQITISYQTAVEKEYTLQGILDFLEQHPQKIVLAIDEFQQI
                     TEYPEKNTESILRTYMQHLQNIRFIFCGSDKRMMTDIFSNVKHPFFASTQFLNLGKIN
                     RDQYAVFIKDSFARNGKHITDEAVDLILDWSKTYTFYTQSVCNMTFSISGKNSDIETV
                     KKACIALLKQGETVYLQYRKLLTPGQWNYLIAVAKEDVVRKITAQSFITRYDIGTPAN
                     SRRLLKALLEKELLLEIQETHAVSYQVYDVFFSRWLQMEY"
     gene            complement(4794..5420)
                     /locus_tag="PLI30_08455"
     CDS             complement(4794..5420)
                     /locus_tag="PLI30_08455"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOI79620.1"
                     /translation="MRLLFDERLILSDVYMKIETGDTVGLLGRNGCGKTCLMRGVYGT
                     LECEKSVIIDNISVYEAYKKPGLIRYLPQHHFIPKHLTLKRVFRDFAVDFERFTELFP
                     VFQLRENTKIGHLSGGMHRLAELYVIIKSDTKFVLLDEPFTHISPVQIETIQSVIGEE
                     RPRKGFLITDHLFKRIQESCNRVYLLSNGKTHTVENDGDLKKLGYIMS"
     gene            complement(5444..5752)
                     /locus_tag="PLI30_08460"
     CDS             complement(5444..5752)
                     /locus_tag="PLI30_08460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI79621.1"
                     /translation="MNFFKKIRLIFSFYNYFVWVSVFIDAACAYTLWQHGISVYKALF
                     WLKLFSMGASFYLVNEYRKQEYFYFYNFGFSKKSLWIITLAFDLLLFFGLMILACQLR
                     "
BASE COUNT         1590 a         1279 c         1412 g         1629 t
ORIGIN      
        1 acgtacgccg gacatttcct ggtggaagcc aaaaacaagg gtttcgatgt atcgcagagc
       61 gtgatcggcc ggtgggtagg ctttcagcag aagctagccc gaaactggac acgtatcgat
      121 tctcatcggg gttattacgg gatttcgatg actgaattgc agcaggctta tcggttgtat
      181 gccttggcat tgagtggaaa tacggagttg ggcgctatga accggatgcg cgaaatcgct
      241 gacttgaact tgcaggcgaa atggcggctt gcggcggctt atgcgttggc cggaaagcct
      301 gatgttgcta actctcttgt tttcaatgct tcggatgcag tggaagatta tcgttccaat
      361 aacgatacgt acggttctcc ggcacgtgat aaggcgatga tcatgcagac gtatctgctg
      421 ctggggaata tcgagaaagc gttgcaattg gctccggata tgtcacgggc gctttcatcc
      481 gattatatct ccacgcaaac cgtcgcgttc agattgatgg caatggcaca gttagcagaa
      541 aaaatgggtt cgggaaatat tgatgtggat tggacattga acggaaagaa aatggctgcc
      601 gtgaatacgc cgcatgcctt tcatcaggtg gatttgaaaa cggcgcccaa tcaatcggta
      661 cagatttcca acaaaggcaa gggcaaagtg tatgcccggc ttaccgcctt catgcaaccg
      721 ttggtggaca cacttcgtgc cgcggagggt tcgttgcggt tgtcggttaa ttaccttgat
      781 gccgcaggta aaccgctgga cgtgaaatcg ctgaaacagg gaacggaatt tacggccgtg
      841 gttactgtcc ggaattcggt ggagcagtcg tttacagatc tggcgttgct gcaggtattt
      901 ccgtcgggtt gggagatttt taacgaacgc ctgacgggaa cccaatcggc tgccgaaacc
      961 tataactacc gggatatccg ggacgaccgg gtattgacct atttcaacct gggagccggt
     1021 caatcggcca cgttccgtgc aaggctccag gcggcgtacc ggggtaacta ttacctgcct
     1081 gccgtatcgt gccaggcgat gtatgaaccg cgtgaacagg cccgaacgag cgggatatgg
     1141 gtagaggtaa gatgattgac aaatttctat ctttaaaacg atgggaaaaa gcaaccttga
     1201 ttatcggttt tatactgatg atggggtatg ctttttccct tcccgatccc ttgttcgatg
     1261 catcatacag tacgttagtc tctgaccgga acggtgaatt gttgggtgca cgcattgcgt
     1321 cggacgggca gtggcggttt ccgccaaccg atagtattcc cgataaatac aaaacctgtc
     1381 tgatggcgtt tgaagaccgc tattttcggt atcatccggg agtgaatccg ctgtcgttgg
     1441 ggcgtgcgat ggtgcaaaat atcaaagcgg gcagggtagt gagcggcgga agtaccctca
     1501 cgatgcaaac cgtacgatta atgcggaaaa acaaacgtac ctacttcgag aaatttattg
     1561 aaattattct cgccacgcga ttggaactgt cgtactccaa aaaacgaatt atcgcgctat
     1621 acgcttccca cgcaccgatg gggggaaatg tggtgggaat tgatgccgcc gcgtggcggt
     1681 atttcggtca cggcgcgcgg tcgttgtcgt gggccgaatc tgccactcta gctgttctgc
     1741 ctaattcacc gtcgttaatg catttcggca ggaacaggga tgcgttactc acgaaacgaa
     1801 atcgtttgct gcaaaaattg cttgacaacg gcacgatttc cgaaaccgat tatcgattag
     1861 ccaccgccga gccgctaccc gaaaacccac attcgttgcc acaaatcgcc cctcatttgg
     1921 tcacacggct ttatttgtct caacccggaa cgcatgtgca atccactatc gacaaaagct
     1981 tgcaactcca agccgataat gtgctggagc gatggaacac cgagttttcg cagaaccata
     2041 ttctgaacct cgcggcgctg attgtcgatt tggaaaaaaa cgaagtcatt tcgtatatcg
     2101 gtaacgtaaa cttcggcaaa caacaatcag gaaatcaagt ggatattatt caatcgtcgc
     2161 gaagtacggg cagtatcctc aaaccttttc tttactgtgc catgttacag gagggcgcac
     2221 tgttacccgg tgagttattg cccgatatac cgataaatgt tggcggtttt tcgcccaaaa
     2281 acttcagttt gcaatacgac ggagcagttc atgcaaatga ggcgctggca cgctcgttga
     2341 acgttccgtc tgtagtctcg ctgcgccggt atggagtgcc gaaattttac gattttctga
     2401 aaaaagccgg attgacaacg cttaaccgcc cagccgacta ttacgggctt tcgcttattc
     2461 tgggcggtgc tgaagggaca ctttgggaag tggcaaacgc gtatgcggat atggcgcacg
     2521 ccgtaaggac ggttgctccc cggaaggggg aattttcact ggtgcatgaa ggacaggatt
     2581 ataggggagg acgggtaaac gcctttacgg atgctggagc ggcgtggttg acattggaag
     2641 cgctgaccaa cgtcaatcgc cccgaagaga tcgactggca gtttatccct tcgatgcgaa
     2701 gggtagcgtg gaaaacagga acgagtttcg gcttccgcga tgcctgggca gtgggtgtta
     2761 caccgaaata tcttgtggcg gtatggacag ggaatgcgag tggcgaaggc cgtcccggac
     2821 tgactggtgc gcgtacatcg gcgcgggtga tgtttgattt gttcaacttg ttgccgccca
     2881 cttcctggtt cgagacacct tataatcatc tgaccgaggt tgttgtttgc cgggagagcg
     2941 gatgcctcgt gggattgcat tgtccgcctt catcggcaga tactgttttt gctccggcaa
     3001 aagcggtgca gggaagcgtc tgcaattatc accaacgggt gcatatttcg gaagatgagc
     3061 gttaccgggt ttatgaacat tgtgctgtag gcaggggaat tcgcccggtt tcgtggtttg
     3121 tgttgcctcc gtcgtgggag tggtattaca aacaacaaca ccctacatac cggtcgcttc
     3181 cgcctttttc gcctgaatgt agcgatggcg catcgggaga ggtgatgcag tttatctacc
     3241 cttatcccaa ctcagtcatc aaaataacca ggcaactgga tggttcgcgt ggaaaagcgg
     3301 ttttcgaact tgctcaccga aacccttcgg cacgggtgtt ctggcacttg gacaatgaat
     3361 acctcggtga aacagcggat gttcatcaga tggagctttc acccgaatcg ggtgcgcatg
     3421 ggttgacggt ggtggatgaa atgggaaatt cgttgacaat caggtttaag gtggagtgaa
     3481 ttttgaatta ctcgatttta tttttaacga attaatattc catttgtagc catcgtgaaa
     3541 aaaagacatc gtacacttgg tatgaaacag cgtgagtttc ttgaatttca agtaacaatt
     3601 ctttttctaa caaggccttt agcaatcttc tcgaattggc gggagttcca atatcatacc
     3661 gtgtaataaa actctgtgcc gttattttcc gtaccacatc ttctttagct acggcaatca
     3721 aataattcca ttgtcccggg gtaagcagtt tacggtattg taaataaacg gtttcgcctt
     3781 gtttaagtaa agcaatgcac gcttttttaa ccgtttctat atccgaattt ttaccggaga
     3841 tggaaaatgt catattacaa acactctgtg tataaaatgt gtaagtctta ctccaatcga
     3901 gaatcagatc gacagcttca tccgtaatat gtttcccgtt cctggcaaat gagtctttta
     3961 taaaaacagc atattgatcc cgattaatct tcccgagatt aagaaactga gttgacgcaa
     4021 aaaaaggatg tttgacattt gaaaaaatgt cggtcatcat tcttttatcg ctcccacaaa
     4081 aaataaatcg gatattctgc agatgttgca tgtatgttcg taagatactt tccgtgtttt
     4141 tttcgggata ttccgtgatt tgttgaaatt catcaatcgc taaaacaatt ttttggggat
     4201 gttgttccaa aaaatcgaga atgccctgta gcgtatattc tttttcaaca gcagtttgat
     4261 aagagatggt tatttgtggc tctcccgata taggatcata cgatatgaca ggacgcaggc
     4321 ttttgagcaa gctgaaaaag tttttcccaa tagtcgtttt tggaggtatc ttcttcaaaa
     4381 ttgcttctat caacaattta ataaaatcac tttgcgtacg ggaggcataa atatctacat
     4441 agatgagttc aaacggttct ccttttagcc cttcaaaaag gcgcattata agacctgttt
     4501 tgcccaatct tcttgccgaa atcaatgttg tatcaatgcc gtttttcaca tttcgctcca
     4561 attctttcag ttcttccacc ctgttgcaaa aaaactcttt cgacagataa ccttttatta
     4621 aaaatggatt ggtcatacta ttttttttca caaagatact atctatttta gattatacaa
     4681 aatagataat cttaaacaga taatttattt cggataaatt cacacaagaa atggcgataa
     4741 aagaaaattt caagaaaaat aaattaaaaa atttatggta acatattgtc aaactacgac
     4801 ataatgtatc ctaatttttt caaatcccca tcattctcaa cggtatgtgt ttttccgttt
     4861 gaaagcagat agacccggtt acaagattct tgaatgcgct taaacaggtg gtcggttatc
     4921 agaaatccct ttcggggacg ttcttctccg ataacggact ggatggtttc gatctgcacc
     4981 gggctgatgt gtgtgaaagg ctcgtcgagc aacacaaatt tcgtatcgga tttgatgata
     5041 acgtacagtt ccgccaatcg atgcattcca ccggaaagat gtccgatttt tgtgttttcc
     5101 cgaagctgaa ataccggaaa aagttctgta aaacgttcaa aatcgacagc aaaatcacga
     5161 aaaacccgtt tcaacgtaag atgtttcgga atgaaatggt gttgcggaag gtatcgtatt
     5221 aaaccgggtt ttttgtaggc ttcataaacg gagatattat cgataataac ggatttttca
     5281 cactccaatg tgccgtaaac accgcgcatc aagcacgttt tcccacatcc gtttcggcct
     5341 aataatccaa cggtatctcc ggtttcaatt ttcatgtaaa cgtccgaaag aataagtctt
     5401 tcgtcaaata acaaccgcat cccacttatt tctaacgtgt gtttcatcgg agttgacagg
     5461 ctaaaatcat taaaccgaaa aacagcaata aatcaaacgc cagcgtaatt atccacagtg
     5521 atttttttga gaaaccaaag ttgtagaagt agaaatattc ctgctttcga tactcgttaa
     5581 ccagataaaa cgatgcgccc atggagaata atttcaacca aaacagtgct ttgtaaacac
     5641 ttattccgtg ttgccacagg gtgtaagcac aagccgcatc aatgaaaacc gacacccaaa
     5701 cgaaatagtt ataaaacgag aaaatcaggc gtatcttttt gaaaaaattc atgttttata
     5761 cgaaagaatt tgagttttgg taagcgttta taacaaagat tcttctaccc attcacggat
     5821 acgctcttcg ctctcttttc ctaattccac aaacgcggga tccatgtgtc ccagaaaatg
     5881 ccaatcagtt tcttccggtc gtttgtaaag
//
LOCUS       DAOSDR010000196         5872 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrimonas sp. isolate AMR_MDS_1826
            NODE_4858_length_5872_cov_4.623861, whole genome shotgun sequence.
ACCESSION   DAOSDR010000196 DAOSDR010000000
VERSION     DAOSDR010000196.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863355
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrimonas sp. (wastewater metagenome)
  ORGANISM  Petrimonas sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Dysgonomonadaceae; Petrimonas.
REFERENCE   1  (bases 1 to 5872)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5872)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:10:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,720
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 35
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5872
                     /organism="Petrimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4858_length_5872_cov_4.623861"
                     /isolate="AMR_MDS_1826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2023866"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            16..1866
                     /gene="mnmG"
                     /locus_tag="PLI30_08465"
     CDS             16..1866
                     /gene="mnmG"
                     /locus_tag="PLI30_08465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008156150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HOI79622.1"
                     /translation="MSSAACSETEAAAAAANLGSKTLLITMDMNKIAQMSCNPAVGGI
                     AKGQIVREIDALGGQMGIVSDRTAIQFRMLNRSKGPAMWSPRVQSDRAKFIQEWRSII
                     ENTPHLHVWQDTVTQLFFQDGEVAGVKTRMGVEFTAKSVVLTNGTFLNGLIHIGSVQI
                     GGGRMSEPASFGMTEQLQAIGFKTDRMKTGTPVRIDGRSVDFSLLEEQKGDDDFHKFS
                     YLDNIQRELTQRSCWITYTSEAVHNLLREGLNDSPLYNGQIQSIGPRYCPSIETKIVT
                     FSDKTSHQLFLEPEGETTHEYYLNGFSSSLPLQIQLNALQAVPAFKNVHIFRPGYAIE
                     YDFFDPRQLNPTLETKRVKNLFFAGQINGTTGYEEAGGQGIVAGINAHINCHGGNPFT
                     LRRDEAYIGVLIDDLITKGVDEPYRMFTSRAEYRILLRQDNADERLTQRAYDLGLASS
                     ERMILLAEKREEVDRIIDFARNFSIKADRINPYLGTLNTAPLKHGVKLIDLLTRPHID
                     LETMSREITPLKELLSNIKNRKEEIIESADIKIKYEGYIKREKLMADKINRLEDIRIK
                     DKFNYNGIQSLSTEARQKLTSINPETIAQASRIPGVSPNDISVLLVLLGR"
     gene            1927..2457
                     /locus_tag="PLI30_08470"
     CDS             1927..2457
                     /locus_tag="PLI30_08470"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019538136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HOI79623.1"
                     /translation="MSIETLTAAVRNIPDFPIKGIQFKDITTLFQSPSHLKELSDILY
                     ERYKEKGITKVVGIESRGFFMGPQLAVRLNAGFVPIRKPGKLPYKVIEEKYTKEYGED
                     TIQIHEDALTKEDVVLIHDDLLATGGTMVAAHKLVKKMDVKKIYINFLIELEALHGRS
                     LFPDEVELDTILRFEI"
     gene            2454..4244
                     /gene="uvrC"
                     /locus_tag="PLI30_08475"
     CDS             2454..4244
                     /gene="uvrC"
                     /locus_tag="PLI30_08475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006800714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HOI79624.1"
                     /translation="MTQEIRDTLKILPQGPGCYQFLDEKGRIIYVGKAKNLKKRVSSY
                     FNKHQENPKTRVLVRNIRQIKYIVVNTEEDTFLLENNLIKELKPRYNVMLKDDKSYPS
                     IVIKNEFFPRVFKTRNIVKDGSKYFGPYTSVAAVDALIEVFRKIYKVRTCRLNLTPEN
                     ISAGKFKVCLEYHIKRCDAPCVGYQSLENYNKNIAEITEILKGNISLIEKQITIEMQH
                     YSDELRFEEAQRLKEKLLLIRNFREKSQVVSNVHYNLDVYGFAEDENAAYINYLHVVN
                     GGIIQAYTFEYKKKLDETPEELLGLGIVEMRQRFGSESKEIMVPFIPDLILSGVEFIV
                     PIRGDKRKLLDLSEKNVKQFKVDKLKKSEMLNPEQRTTRILKTVQKDLHLKEIPWHIE
                     CFDNSNIQGTDPVAACVVFKKAKPSKKDYRHFNIKTVVGPDDYASMAEIIHRRYSRAV
                     NEGHSLPQLIVIDGGKGQLHAATESLQKIGLYGKIAIIGIAKRLEEIYFPEDSVPLYI
                     DKNSETLKLIQQLRDEAHRFGITHHRNRRSKSQVTSELDQIKGIGKETKKKLLSHFKS
                     VKRIKETREEEIASVVGKSKGKLITDFFKK"
     gene            4269..4721
                     /gene="dtd"
                     /locus_tag="PLI30_08480"
     CDS             4269..4721
                     /gene="dtd"
                     /locus_tag="PLI30_08480"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005843922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HOI79625.1"
                     /translation="MRLLIQRVSEASVTINSLKKSSITKGLLLLVGIEESDTKEDIDF
                     MCKKTIYLRIFDDQNGVMNKSVLETGGEILVVSQFTLLASTKKGNRPSYIRAAKPDIS
                     IPLYKQFCETLSEMLRKPVQTGEFGADMQVQLINDGPVTIWLDSKNKE"
     gene            4723..5046
                     /locus_tag="PLI30_08485"
     CDS             4723..5046
                     /locus_tag="PLI30_08485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018711496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HOI79626.1"
                     /translation="MTIQEAQQLVDRWIKTYGVRYFSELTNLAILTEEVGELARIMAR
                     TYGEQSFKDSDLEKNLADEMADVLWVLLCLANQTGVDLTDALQKNFVKKTKRDNNRHK
                     ENSKL"
     gene            5069..>5872
                     /gene="deoC"
                     /locus_tag="PLI30_08490"
     CDS             5069..>5872
                     /gene="deoC"
                     /locus_tag="PLI30_08490"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006800708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HOI79627.1"
                     /translation="MNTTNKYTDALKKYPFALSADQITESINKIIADGFNGNNTQEVY
                     RKIYSCIDLTTLNSTDSREEVWKFTEKVNAFEDINPELENVASICVYPNFAKTVKEAL
                     TVDVKIACVSGGFPSSQTFSEVKIAETALSVADGADEIDTVINLGLFLDEEYEEMCEE
                     LTEIKDACHGATLKVILETGALKSAENIHKASVLSLYSGADFLKTSTGKGYPGASPEA
                     AYVMCKAIKSYYEKTGNKAGFKVSGGVSSVEDAVKYYTLVKEILGEEWCS"
BASE COUNT         1906 a         1273 c         1285 g         1408 t
ORIGIN      
        1 acccgcgccg gttgtctgtc ctccgccgcc tgctccgaaa ccgaagccgc tgccgccgcc
       61 gcaaatttgg gctcaaaaac gttgttgata acaatggata tgaataaaat cgcccaaatg
      121 agctgcaacc ctgcggttgg aggtattgca aaaggacaga ttgtgcgtga aattgatgcg
      181 ttgggtgggc agatgggtat tgtaagcgac agaacagcga tccagttccg tatgcttaat
      241 cgttccaaag ggcctgccat gtggagtcct cgtgtacaaa gcgaccgggc aaaattcatc
      301 caggaatggc gttcgatcat cgaaaatacg cctcatctgc atgtatggca ggacaccgtt
      361 acgcagcttt ttttccaaga cggtgaggta gctggcgtaa aaacccgtat gggggttgag
      421 tttaccgcca aatcggttgt tctgaccaac ggaacattcc ttaacgggct gattcacatc
      481 ggcagcgtac aaataggcgg cggacgcatg tcggaaccgg cctctttcgg gatgacggaa
      541 caattgcaag caatcggatt taaaaccgac aggatgaaaa cgggtacgcc tgtacgcata
      601 gacggtagaa gcgttgattt ttcactgctg gaagaacaaa agggggacga tgattttcac
      661 aaattctcct atttggataa cattcagcgt gaactcaccc aacgcagttg ctggataacg
      721 tatacctcgg aagccgtgca caacctgctt cgggaaggct tgaatgattc accgctctat
      781 aacggccaga tccaaagtat cggaccgcgc tattgtccaa gcatcgagac caagatcgtc
      841 acgttttcag acaaaacgag ccatcagctc tttcttgaac cggaaggcga aaccacccac
      901 gaatactacc tcaatggatt ctcctcttcc ctacccctgc aaattcagct aaacgcactt
      961 caagctgttc ccgctttcaa gaacgtacat attttccggc cgggatatgc tatcgagtac
     1021 gatttcttcg atcctcgcca gctcaatccg acacttgaaa caaaacgggt gaagaacctg
     1081 ttttttgccg gacagatcaa cggcactacc ggctatgaag aagcgggggg acaaggcatt
     1141 gtagccggaa taaacgccca tatcaattgc catggcggaa acccgttcac actgcgcagg
     1201 gatgaggcct atatcggtgt actcattgac gacctcatca caaaaggagt ggatgaaccg
     1261 tacagaatgt ttacgtcgcg cgccgaatac cgcatcctgc ttcgccagga caatgccgac
     1321 gagcgactca cacagcgagc ttatgacctt gggcttgctt cttcagaaag aatgatttta
     1381 ctggcagaaa aacgagaaga agtggacagg attatcgatt tcgcacgcaa tttctccatc
     1441 aaggcagacc gtataaaccc atacctgggg accttgaata cggcaccgtt aaagcatggc
     1501 gtgaagctta tcgatctgct tacccgcccg catatcgacc tggaaacaat gtccagggag
     1561 attacccctt taaaggagct gttgtcgaac atcaaaaacc gaaaggaaga aattattgaa
     1621 tctgccgata taaaaataaa gtacgagggc tatatcaaac gtgaaaagtt gatggccgat
     1681 aagataaaca ggctggaaga tatccggatc aaagacaagt tcaactacaa tgggattcaa
     1741 tccttatcaa ccgaagcacg gcaaaaatta acgtctatca accccgaaac cattgcccag
     1801 gccagccgaa tccccggagt atccccgaac gacatatcgg ttttacttgt gcttctggga
     1861 agatagaata caaatgttcc acgtggaaca atctcaaatt atacacacat ttataattat
     1921 acaaaaatga gtattgaaac attaaccgct gccgtcagga atatccctga ctttcctatt
     1981 aaaggaatac aattcaagga catcactacg ctgttccagt cgccatcgca tttaaaagaa
     2041 ctgtccgaca ttctttacga aaggtataaa gaaaaaggca ttaccaaggt ggtaggtatt
     2101 gaatccaggg gttttttcat ggggcctcaa ttggcagtac ggttaaacgc aggatttgtt
     2161 ccaattcgta aaccgggtaa acttccttac aaggttattg aagaaaagta taccaaggag
     2221 tacggagaag acaccatcca gattcacgaa gatgcactaa caaaagaaga cgttgttttg
     2281 attcacgacg atttgttggc taccggaggg acaatggtag ctgcccataa actggtgaaa
     2341 aaaatggacg ttaagaaaat ttacatcaat tttctgatag aactagaagc actccacggg
     2401 cgctccctgt tccccgatga ggtggagttg gatacgattc ttagatttga aatatgacac
     2461 aagagatacg ggatacacta aaaatacttc ctcagggacc aggctgctac caatttttag
     2521 acgaaaaagg cagaataatc tatgtgggca aagccaagaa cctgaagaaa agagtatcct
     2581 cgtatttcaa taaacaccag gaaaacccta aaacccgcgt tctcgtccgg aatatccgac
     2641 agataaaata tattgtggtt aatacggaag aagacacatt tcttctcgag aacaatctta
     2701 tcaaagaact gaagccacgc tacaacgtga tgctgaagga cgataaatcc tacccttcaa
     2761 tcgttataaa aaacgaattt ttcccacggg tttttaaaac aagaaatatc gtaaaggatg
     2821 gctcaaaata ctttggcccg tatacttccg ttgctgcggt cgacgccctg attgaggttt
     2881 ttcgaaaaat ttacaaagta agaacctgca ggctcaattt aactcccgaa aacatctctg
     2941 ccgggaaatt caaggtttgc ctcgaatatc acattaaacg atgcgatgct ccctgcgttg
     3001 gatatcaatc actggaaaat tacaacaaaa acattgcaga gattaccgaa attctgaaag
     3061 gaaacatctc ccttattgag aaacaaataa cgattgaaat gcagcactac tccgatgagc
     3121 tccgttttga agaagcacag cggctcaagg agaaacttct gctgatccgg aatttcaggg
     3181 agaaatcgca agtggtaagc aatgttcatt ataacttgga tgtctatgga tttgcggaag
     3241 acgaaaatgc ggcttacatc aactatctcc acgtggtgaa tggcggtata atccaagcgt
     3301 acacttttga atacaagaaa aaactggatg aaacacccga agaactgctg gggttaggaa
     3361 ttgtggaaat gcgtcaacgc ttcggtagtg aatcgaaaga gatcatggtc cccttcattc
     3421 ctgatcttat tctttccgga gttgaattta tcgttcctat acggggagac aagcgaaaac
     3481 tcctggacct ttccgagaag aacgtcaagc agtttaaagt agacaaactg aaaaagtcag
     3541 agatgctgaa tccggagcag cgaaccacac gcattttaaa gacggttcag aaagatctgc
     3601 atctgaaaga gattccatgg cacatcgaat gttttgacaa ttcaaacatt caaggtacag
     3661 atcccgtggc agcttgtgtg gttttcaaaa aagcaaagcc atcaaaaaaa gattaccgcc
     3721 atttcaacat taaaaccgtc gtaggtcccg atgattacgc ctcgatggcc gaaattattc
     3781 acagacgcta ttctcgtgca gttaacgaag gtcactcttt gcctcaactc attgtaattg
     3841 acggcggtaa agggcaactc cacgctgcaa cagaatcgct tcagaaaatt ggactttacg
     3901 gcaagatcgc cattattgga attgcaaagc gattggaaga aatatatttc cctgaagact
     3961 ccgtcccgct ctacattgac aaaaattctg agacactaaa actcattcaa caacttcgcg
     4021 acgaagctca tcgtttcggt attactcatc accgaaacag aagaagtaaa tcgcaggtga
     4081 cttccgaact ggatcaaata aaaggaatcg gaaaagaaac caaaaagaaa cttctttcac
     4141 actttaaaag cgtgaagcgc ataaaagaaa cccgtgaaga agaaattgct tcagttgtcg
     4201 gaaaaagcaa aggaaaactg attaccgatt tttttaaaaa ataataagat aaaatattgc
     4261 tgacctttat gcgattgcta attcaaagag tatcagaagc atcagtaacc attaactccc
     4321 ttaaaaagag ctctatcacg aaagggttgt tactcctggt aggtattgaa gaatccgata
     4381 caaaagaaga tatcgatttt atgtgtaaaa agaccattta tttacgtata tttgacgacc
     4441 aaaacggggt gatgaataaa tcagttttag agaccggagg agaaatttta gtggtctctc
     4501 aatttactct tctcgccagc actaaaaaag ggaatcgtcc ttcttatatt cgcgccgcca
     4561 aacccgatat ttccataccc ttgtataagc agttttgcga aacattatcg gaaatgctca
     4621 ggaaaccggt acagaccggt gaatttggtg cggatatgca ggttcagctg attaatgacg
     4681 gtcccgtgac tatttggcta gattcgaaaa acaaagaata agatgaccat tcaggaagct
     4741 caacaattag tagacaggtg gatcaagacc tacggagttc gttatttcag cgaacttacc
     4801 aacttggcca ttctcaccga agaagtggga gaactggctc gaattatggc acgcacgtac
     4861 ggtgaacaat cgtttaaaga ttctgatctg gagaaaaacc ttgccgatga aatggcggat
     4921 gtactgtggg tgttgctttg cctggccaac caaaccggag tagatttaac ggatgctttg
     4981 caaaaaaact tcgtaaaaaa aacgaaaaga gacaacaaca ggcacaaaga aaattctaaa
     5041 ttataacaaa aaaaaactaa ttgataatat gaatactaca aacaaataca ccgatgcatt
     5101 aaagaaatat ccgtttgctc tttctgctga ccaaataaca gaaagcatca acaaaatcat
     5161 tgcagatgga ttcaacggga acaatacgca ggaagtatac cggaaaattt attcttgtat
     5221 cgatcttaca acactcaatt caacggattc cagggaagaa gtctggaaat ttacggagaa
     5281 agtgaacgct tttgaagata taaatcctga actagagaac gttgcttcca tttgcgtata
     5341 cccaaacttt gccaaaaccg taaaagaagc gttgaccgtt gatgtaaaaa ttgcctgtgt
     5401 atcgggtgga tttccaagtt cccagacctt ctccgaagta aaaattgcag aaacagcact
     5461 ttccgtagca gacggagccg atgaaataga cactgttatc aacctgggac tgtttttgga
     5521 tgaggaatac gaggaaatgt gtgaagagct tacggagata aaagatgcgt gccacggggc
     5581 aaccctgaaa gttattctcg aaaccggagc cttgaaatct gcagagaata ttcataaggc
     5641 ctccgtccta tcgctctatt cgggtgcaga tttcttaaaa acatccacgg gaaagggata
     5701 tcccggtgct tctccggaag ccgcctatgt aatgtgtaag gccattaaat cctattacga
     5761 aaaaaccggg aacaaagccg ggtttaaggt ttccggcggt gtgtcttctg tagaagatgc
     5821 agtaaaatat tatacccttg taaaagaaat tctgggtgaa gaatggtgct cc
//
LOCUS       DAOSDR010000197         5841 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrimonas sp. isolate AMR_MDS_1826
            NODE_4886_length_5841_cov_3.641203, whole genome shotgun sequence.
ACCESSION   DAOSDR010000197 DAOSDR010000000
VERSION     DAOSDR010000197.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863355
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrimonas sp. (wastewater metagenome)
  ORGANISM  Petrimonas sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Dysgonomonadaceae; Petrimonas.
REFERENCE   1  (bases 1 to 5841)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5841)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:10:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,720
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 35
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5841
                     /organism="Petrimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4886_length_5841_cov_3.641203"
                     /isolate="AMR_MDS_1826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2023866"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(58..1104)
                     /locus_tag="PLI30_08495"
     CDS             complement(58..1104)
                     /locus_tag="PLI30_08495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI79628.1"
                     /translation="MDIQSKKIWQQACGDTDRNYSDVCLEWDVILNGPGYAGTYPSCE
                     QILKEHGWSSKKRTDIWRFADQMKDGDIVVLRLGTSDILAVGIIVGDYQWSNIFADID
                     GWDLQHYRRVRWLWKTNGQHKKFNTYTLKQGDTTQELTSDEVKQWLVSLSFSNTEMNR
                     PLVELPSYEPKQISHEEIGEYLFEQGVSSNSIETLIKEFDELIRIAKWYKDKDAPSEF
                     ETVAYLVVPILRALGWTPQKMAIEWRNVDLALFDQLPRDDKNLAVVVEAKKKGNACLT
                     AKSQAQGYAQGKDNCKRLIVTDGLRYGTYLKENNEYKLYAYFNLLSLTDGHPIYNCKG
                     VKEALRIMTPEWRE"
     gene            complement(1307..1435)
                     /locus_tag="PLI30_08500"
     CDS             complement(1307..1435)
                     /locus_tag="PLI30_08500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI79629.1"
                     /translation="MNNRQPDSVSERKAGRKTADNNLDKQQNGAKGNLCFFSIISP"
     gene            complement(1428..2003)
                     /locus_tag="PLI30_08505"
     CDS             complement(1428..2003)
                     /locus_tag="PLI30_08505"
                     /inference="COORDINATES: protein motif:HMM:NF024986.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HOI79630.1"
                     /translation="MTPDYIEDKTFDGIDIKTKTLTKGEYQNCHFRNCDFSNADLSHY
                     IFSACEFWGCNFSLAKLTKTAFRKIIFKECKMVGVHFDSCNEFGLSFRFENCLLNHAS
                     FYKTKIKKTTFRNTQLQEVDFTGCDLTGSLLDHCDLTRAIFDNTILEKADFRTSSNYS
                     IDPEINRMKKAKFSIHGIAGLLEKYDLEIDE"
     gene            complement(2029..3546)
                     /locus_tag="PLI30_08510"
     CDS             complement(2029..3546)
                     /locus_tag="PLI30_08510"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HOI79631.1"
                     /translation="MKLNLFFRIFLWMVLFATQTHAQDLSLFAQDSLDRYIGQALEEW
                     RIPGAAVYIEKDGEILLQKGYGVSNWNTRNRVDEQTVFPIASVSKTFTGTLLATLEAE
                     GKVSLDDLVKKWLPNFSMKDKMYEEQITLADILSHRSGWKTFQGDLLNTESSMDYPAM
                     IQKFSRQTPAYPFRTRFGYSNFGFMIAGECVKNIAQQDFNTCLQNRFLTPLGMKRTLV
                     FEDEIKNEKNITAAHTLVNDTITVLTRDKVEPYSHGGIYASIQDLGIWVNTLLNKGNL
                     KEKSIIPESAIAKMWQSHTIIGKSRAADRQMYFKTYGLGWEIIQYQQAEVMQHNGAYA
                     GALTSLALVPDINLGIVILTNQDNHLLHETLKWQIIDAFLQKKAPNYTLSIIERQKKR
                     KAESTHRPQENNAKTENFATSLEAITGTYLCEYYGKAYIQKNNGEYILTLEHHPQLQG
                     ILSPSQKSQLNCTYNHPMFGSVQFPFVVEKNKVKSFTLFVDSFVEADGYEFKKVK"
     gene            complement(3716..4066)
                     /locus_tag="PLI30_08515"
     CDS             complement(3716..4066)
                     /locus_tag="PLI30_08515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019538545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2200 domain-containing protein"
                     /protein_id="HOI79632.1"
                     /translation="MNNIKIFKTSFARIYPLYIQKAERKGRTKAEVDAIIFWLTGYDE
                     QTLQQQIDRNSDLETFFAQAPHLNPNVSKITGVICGYRVEQIEDELMQKIRYLDKLVD
                     ELAKGKAMEKILRQ"
     gene            complement(4333..4893)
                     /locus_tag="PLI30_08520"
     CDS             complement(4333..4893)
                     /locus_tag="PLI30_08520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005924573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdeI/OmpD-associated family protein"
                     /protein_id="HOI79633.1"
                     /translation="MDKILTFADRQAFREWLGQNGTESDGLWLRFSKKGKFDTLTAAE
                     ALEEALCHGWIDGQLQFLDDHTYKKYFARRLPKSKWSVKNKELAQTLIEKGLMTRQGL
                     EAIERARENGSWDHAKRILIDDEQIQMFKEIIQPHEPAYTNLLAMSHSVQRTYTGFYL
                     DAQTDKTRQTRLEKIIDRLNRNLKPM"
     gene            complement(4922..5314)
                     /locus_tag="PLI30_08525"
     CDS             complement(4922..5314)
                     /locus_tag="PLI30_08525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006843800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HOI79634.1"
                     /translation="MDKRTVTETIIALETAALDAWHKGNPSPYLELYSKDFTYFDPAH
                     EKRLDGWDKIKVFYESMRGKVNMDKFEIINPVVQSTGTMAVLTYNLRSYSGETLWKEN
                     CTEVYRLEENNEWKIIHSHWSLTQPAID"
     gene            complement(5602..>5841)
                     /locus_tag="PLI30_08530"
     CDS             complement(5602..>5841)
                     /locus_tag="PLI30_08530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI79635.1"
                     /translation="LSTYLTHDRHWKIFLASLIMIVIGVFFLFYFSSLGGFGGTSTLS
                     WWWGTLILPYPLGWLMAIITLIVRAANKSKQQVNT"
BASE COUNT         1433 a         1208 c         1254 g         1946 t
ORIGIN      
        1 gcgctccccg tcagtactca ctggcggggc agtttattcg attttagccc gtttttctta
       61 ttctctccat tcaggtgtca ttatgcgtaa tgcttctttt actcctttgc aattataaat
      121 tgggtgtccg tccgttaaac tcaaaagatt aaagtaagca taaagtttat actcattgtt
      181 ttccttaaga taggtgccat atcgaagtcc gtctgttacg attagtcgtt tacaattgtc
      241 tttcccttgt gcgtaacctt gtgcttggct ttttgctgtc aaacaagcgt tgcctttctt
      301 ttttgcttcc acaactactg ctaagttttt gtcatctctt ggaagctggt caaataaagc
      361 aaggtcaaca tttctccatt caattgccat tttttgaggt gtccaaccaa gtgctcgtag
      421 aattggaaca actaaatatg caacggtttc aaattctgag ggagcatctt tgtctttata
      481 ccattttgca attcgaatga gttcgtcaaa ttcttttatc aaggtctcga ttgaattgct
      541 tgaaacacct tgttcaaata aatactctcc aatttcttcg tgtgaaattt gttttggttc
      601 atagctcgga agttcaacta atggtctgtt catttccgtg ttggaaaatg ataaacttac
      661 taaccattgt tttacttcat cagaagtcag ttcctgtgtg gtgtctccct gttttaaggt
      721 ataagtattg aatttcttat gttgtccatt tgttttccaa agccatctaa cccttctgta
      781 atgctgtaaa tcccagccgt caatgtccgc aaaaatgtta ctccattgat agtcgccaac
      841 gataatacca acagctaaaa tgtcagaagt gccaagtcgt aaaacaacaa tgtctccgtc
      901 tttcatttgg tctgcaaacc gccaaatgtc agtccgtttt tttgaactcc atccgtgttc
      961 cttgagaatt tgttcacacg aaggataagt acctgcgtag cctggtccgt ttaggataac
     1021 gtcccattca aggcaaacat ctgagtaatt tctatcagtg tcgccacacg cttgttgcca
     1081 tatttttttg ctctgtatat ccattcgttg gtgtcgtctt aaaatgcctt ataactcatt
     1141 tatatatatg tatctcatcc gtttgtaaac acttacaaac agttattccc ggatcatttt
     1201 tgaggagatg gtcgtggctg ggtggtcgat gcggttgaat ggtcatgttc gtccggttta
     1261 gatttggctc caaaatacag aactgaactg gataaaacaa tagttgtcag ggagaaatga
     1321 tgctgaaaaa gcagaggttg ccttttgcgc cgttctgctg tttgtccagg ttattgtccg
     1381 ccgttttccg gccagctttt ctttccgaaa cactgtcggg ctgccgatta ttcatcaatt
     1441 tccagatcgt acttttccag aaggcctgca attccgtgga tggaaaattt tgcctttttc
     1501 atccggttaa tttccgggtc aatcgaatag tttgaggagg tgcgaaaatc tgctttttcc
     1561 aggatggtat tgtcaaaaat tgccctggtc aggtcgcaat ggtccagcag cgagccggtc
     1621 aggtcgcacc cggtaaaatc gacttcctgc aattgggtat tccggaacgt tgtttttttg
     1681 atttttgtct tgtaaaacga cgcgtggttt aacaggcaat tttcaaaacg gaaggagagg
     1741 ccaaattcat tacaactgtc gaaatgcacg cccaccattt tacattcctt aaaaatgatc
     1801 ttcctgaatg cggtttttgt cagtttagcc aaactgaaat tacagcccca gaattcacag
     1861 gctgaaaaaa tataatggga gaggtcggca tttgagaagt cgcagttgcg gaaatgacag
     1921 ttttggtact ctccttttgt caatgtcttg gttttgatat caatgccgtc gaaagttttg
     1981 tcttctatat agtcgggtgt cataggttgc tgctgctttt agcgttgttt atttcacttt
     2041 tttaaactca tagccgtctg cctctacaaa gctgtctaca aacagggtga atgattttac
     2101 cttgtttttt tcaacaacaa atggaaactg cacgctgcca aacatcgggt gattgtaggt
     2161 gcaatttaat tgggattttt gggaaggaga aagaatacct tgcagttgcg ggtgatgttc
     2221 cagtgtcaga atatactctc cattgttttt ttggatatag gctttgccgt agtattcaca
     2281 cagataggtg cctgtgatgg cctccagact ggtggcaaaa ttttctgttt ttgcgttatt
     2341 ttcctgtggc cgatgagtgc tttccgcttt tcgtttcttt tgtcgttcaa taattgaaag
     2401 ggtatagttt ggagcctttt tctgtaaaaa agcatcaata atttgccatt tgagcgtttc
     2461 atgcaaaaga tgattgtcct ggttggtcag gatcactatg ccaaggttaa tgtcggggac
     2521 aagggcgagc gaagttaaag cccctgcata ggccccgttg tgttgcatca cttccgcctg
     2581 ctgatattga ataatctccc aacccaggcc gtatgtttta aagtacatct gacgatcggc
     2641 agctctcgat tttccaatga tggtatgact ttgccacatt ttagcgattg cactttccgg
     2701 gatgatgctt ttttccttca aatttccttt gttcagcaac gtatttaccc agattcccag
     2761 atcctgaatg cttgcgtaaa tgccaccgtg actgtagggt tccactttgt cgcgtgtcag
     2821 tacggtaatg gtatcgttta cgagggtgtg cgccgccgta atgttttttt catttttgat
     2881 ttcatcttca aagaccaacg tacgtttcat gccgaggggc gttaaaaaac ggttttggag
     2941 gcaggtgtta aaatcctgct gggcgatgtt tttaacacat tctccggcta tcataaatcc
     3001 aaaattggaa tagccaaacc ttgttcggaa agggtaggcg ggggtctgcc ggctaaattt
     3061 ctgtatcata gcaggataat ccattgagga ctccgtgttg agcaggtcgc cctgaaaagt
     3121 tttccaaccc gaacggtgtg agaggatatc ggcaagggtg atttgctctt catacatttt
     3181 atctttcatg ctgaaattgg gcaaccattt tttcaccaaa tcatctaatg atactttgcc
     3241 ttcggcttct aaggtggcca gaagtgtccc ggtaaaagtt ttggataccg atgctatcgg
     3301 gaaaacggtt tgttcatcaa ccctgtttcg ggtgttccaa ttgctcacgc catatccttt
     3361 ctgaagtaat atttccccgt ctttttcgat gtaaacggca gcgcccggga ttctccattc
     3421 ttccagtgct tgtccgatgt acctgtccag gctatcctgg gcaaacaggc ttaaatcctg
     3481 ggcgtgtgtt tgggttgcaa agaggaccat ccacaggaat attctaaaaa agagatttaa
     3541 tttcataacc agtaatttta agcgttaagc aagagcgttg ttttttgaaa tagtctttga
     3601 cttctcacgt tggcgtatcc gtttttttag gtaccaatgc tttaaaatgt accggggtcc
     3661 gaaaaggtta taaaagccag gtgtgtgatt ccaaggcgtt ttgttttacg tttgtttact
     3721 gccgtaaaat cttttccatt gctttgcctt tagccaattc atcaaccaac ttatccaaat
     3781 aacgaatctt ttgcatgagc tcgtcttcta tttgttcgac acgatacccg caaattacac
     3841 cggtaatttt ggaaacattg ggattcaggt gcggtgcctg ggcgaagaaa gtttccaaat
     3901 cgctgtttcg gtcgatttgt tgttgcaaag tttgttcatc atatccggtc agccaaaaaa
     3961 tgatcgcatc aacttcagct ttcgtacgtc cctttctctc tgctttttga atgtacagcg
     4021 gatagatcct ggcaaaagag gtcttaaata tttttatatt gttcatacga ctgtcgttta
     4081 gcgatcaaat ataattgatt ggtggtctcc gattcattat ttttcatcgt gagttaggtt
     4141 tttgaaattt aggaaaatgg tttagcggta tgaccagaaa gggattgcgg gcggtttcct
     4201 gtcaagttac ccaaaagttg aagcgggcta ctacccttga ataacctgct cttccgacaa
     4261 tttttcatac ggagtggggt gcgttcgtgt atgttataac ttttttcagt aatggcttat
     4321 aaactactta cctcacatgg gcttcaaatt tctattcagt ctgtcaataa tcttttctaa
     4381 ccgtgtttga cgggttttgt ccgtttgggc atcaagataa aatccggtgt atgttcgttg
     4441 taccgaatgc gacatcgcca gtaaattggt ataagcaggc tcatggggct gaatgatttc
     4501 cttaaacatc tgtatttgtt catcgtcaat aaggatccgt ttcgcatgat cccatgaacc
     4561 gttttccctg gcgcgttcga tggcttctaa accctgccgg gtcattaatc ccttttcgat
     4621 aagggtttgt gccagctctt tgtttttcac cgaccatttg cttttaggta gacgtcgggc
     4681 aaaatacttt ttataagtat ggtcgtcaag aaattgcagt tgtccgtcta tccatccgtg
     4741 acaaagggct tcttccaatg cttcggcggc agtgagtgtg tcaaattttc cctttttact
     4801 gaagcgtaac cataggccat cgctctctgt gccattctga ccaagccatt cccgaaaggc
     4861 ttgtcggtca gcgaatgtca atattttatc cataaacaat ccccaaagtg tgaccgtgta
     4921 attaatcaat agcaggttgg gtcaacgacc aatggctgtg gatgattttc cactcgttgt
     4981 tttcttccag ccggtacact tcggtacaat tctctttcca gagcgtttcg cctgagtagg
     5041 aacgcagatt gtaggtcaga acggccattg taccggttga ttgcacaacg ggatttatga
     5101 tttcgaattt atccatgttc acctttcccc gcatgctttc atagaacact tttatcttgt
     5161 cccaaccgtc gagccgtttt tcatgggcag ggtcgaaata ggtgaagtct tttgaataga
     5221 gttcgagata gggagaggga ttacccttgt gccaggcatc gagggctgct gtctctaatg
     5281 caatgattgt ttctgtaaca gtacgcttat ccatgatgtt ttatattaaa ttgaaaatca
     5341 ttctgtttta agactcttcg tgggtctttt gtttgccacc aagggcaaag attaatattg
     5401 tcgaatcagt cattcagttg gcatcttgca tgttttggcg cttggcgcag tggcggattt
     5461 cggagcacaa aactgtctat acgcaacaat atttgtttga agtacaaatg ttcgtagtcg
     5521 cactgtccct caacttttgg gtagctgctg ttggcagctg gcgttcttgt cttcaaggtc
     5581 atgataaaag aataattctg cttatgtatt gacttgttgt tttgatttat tggcagccct
     5641 gacaattaac gtaattattg ccattaacca tccaagcgga taaggtagta ttagtgtacc
     5701 ccaccaccaa gatagtgttg acgtgccgcc aaagcctccg agtgacgaaa aataaaacag
     5761 aaaaaacaca ccgattacaa tcattatcag tgatgcaagg aaaattttcc aatgtcgatc
     5821 gtgtgtcaga tatgttgata g
//
LOCUS       DAOSDR010000198         5837 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrimonas sp. isolate AMR_MDS_1826
            NODE_4890_length_5837_cov_3.977516, whole genome shotgun sequence.
ACCESSION   DAOSDR010000198 DAOSDR010000000
VERSION     DAOSDR010000198.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863355
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrimonas sp. (wastewater metagenome)
  ORGANISM  Petrimonas sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Dysgonomonadaceae; Petrimonas.
REFERENCE   1  (bases 1 to 5837)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5837)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:10:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,720
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 35
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5837
                     /organism="Petrimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4890_length_5837_cov_3.977516"
                     /isolate="AMR_MDS_1826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2023866"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1504)
                     /locus_tag="PLI30_08535"
     CDS             complement(<1..1504)
                     /locus_tag="PLI30_08535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007654123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HOI79636.1"
                     /translation="MIQQNFIKIYEESFKTNWELPALSNYKDQETLSYGQLAEKIARI
                     HILFTELGIEEGDKVALIGKNHTSWSVIFLATFTYGAVIVPILHEFNPESMEHIIAHS
                     ESKCIFINENIWENLDKGNIKLPVFSLPSFNLLQSENKKTRNLTGKIDALFAKKYPAG
                     FHPEDVVYADVDNDDVICLNYTSGTTGFSKGVMLTANNFAGNITYAHTLELLFPGERD
                     LAFLPMAHAYGCAFDFLYALSTGVHVTLLDIIPSPQNLIKAFQEIKPNIIITVPLIFE
                     KIYKKRILPIVDKPIMKTLLQIPGINNMIYSKIRKSLIDSLGGNFREVIIGGAALGKD
                     IEAFFYKIKFPFTVGYGMTECAPLISYDHHYEFVPTSCGSVLAGIMEARIDSPDPENV
                     PGEIQVRGENVMKGYYKNPEATKAAFTKDGWLKTGDSGIMRGRRLFIKGRIKTMILGP
                     SGQNIYPEEIEAKLNNLPYVAESLVVERENKLVALVYPDFAALEASGLTAG"
     gene            1726..3045
                     /gene="mtaB"
                     /locus_tag="PLI30_08540"
     CDS             1726..3045
                     /gene="mtaB"
                     /locus_tag="PLI30_08540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004288446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N(6)-L-threonylcarbamoyladenosine(37)-C(2))-
                     methylthiotransferase MtaB"
                     /protein_id="HOI79637.1"
                     /translation="MIDQTVFENKTAAYYTLGCKLNFAETSAIGKQLLHAGVLRARKG
                     QKADICVINTCSVTELADKKGRQAIRRMIQSHPDAFVVVTGCYAQLKPDEVASIDGVD
                     LVLGSEQKLDVIRYLDDLKKKDKGEVVTSKTHRIRTFVPSCSADDRTRYFLKVQDGCD
                     YFCSFCTIPYAGGRSRNGSIADLVKQAQDVVVQGGKEIVLTGVNIGDFGHTTGETFFD
                     LIKALDEVEGIERFRISSIEPNLLTDEIIDFVAGSKRFAPHFHTPLQAGSDAVLKLMK
                     RKYDTALFRRKVERIKSAMPHAFIGVDVIVGLRGETDAYFEEGRDFIESIDFSQLHVF
                     TYSERSGTQALEIGHVVDPRTKHNRSKELHDISDRKLRSFYESQKDGFRKVLFEHTRH
                     GNRMHGFTENYVRLYADYNPLLVNKITGVKIGEYNEDEMAMKAVFGE"
     gene            3057..3959
                     /locus_tag="PLI30_08545"
     CDS             3057..3959
                     /locus_tag="PLI30_08545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019540438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HOI79638.1"
                     /translation="MTGKNRKGIGFYPLYGATYLMAMLPFRVLYILSDVLYIVVYYLV
                     GYRRKIVRKNLLRSFPRKKEEEILQIEKKFYRHLCDYFVETVKTLRISEKEIRKRMVF
                     KNPEIVNRLTKDGNTCLLSLGHYGNWEWVPSIALYLLPGVEPGQVYKTLKSKAFDELF
                     LKIRSRFNPLSIEMKTAYRTIIRKRNEGQAMVIGFLTDQRPRKHNNEYWATFLNQDTL
                     LQTGMERIARQLGLAVVYLDIKKVERGHYVGNFSVITADASAEEEFTVTEKYTRKLEE
                     TILNDPAYYLWSHNKWSRSKKQEA"
     gene            3956..4993
                     /locus_tag="PLI30_08550"
     CDS             3956..4993
                     /locus_tag="PLI30_08550"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007566901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOI79639.1"
                     /translation="MKTSVVILNWNGRKLLERFLPVVLQHTLDHQTEVVVADNGSSDD
                     SVDFLNTRFPQVPLIVLDKNYGFAEGYNKALRQVDAEYVVLLNSDVETTENWLSTMIS
                     YLDEHPGVSALQPKILAQRNKTGFEYAGACGGFLDRFGYPFCRGRILDNVEQDRGQYD
                     TVIPVFWATGACLCIRRKDYFEAGGLDARFFAHMEEIDLCWRLNARGKRIECLPQSVV
                     YHVGAASLEKENPRKTYLNFRNNLLMLYKNARIRSLLVVFTVRLILDALAFLHLLSMG
                     KFDNAKSVIEAHRDFFRMMPGFRHDRRENLKQRLVTDIPTKFKGSILWNYYVKRKKTY
                     SDLPLTPVTQN"
     gene            complement(4980..5684)
                     /locus_tag="PLI30_08555"
     CDS             complement(4980..5684)
                     /locus_tag="PLI30_08555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002559160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin family protein"
                     /protein_id="HOI79640.1"
                     /translation="MRKIFLLVIVLSALSDPTYGQQQKLQNRPYADHKIFHLGFTIGV
                     HTQDLMLTQSGHTDENGEVWFSEIPSYSLGFSVGIITDLYLNRFMNLRAIPTLHLGSK
                     NLVFKEQVSGETFKTGLRNNYVTLPLQVKLASTRINNYRPYLVAGTYGSIELASRKNQ
                     PILLKPYDYGVEIGIGCDFYLPYFNLCPELKFCFGLNDMLEKDRSDLTDKELIKYPLS
                     LARATQRMIVLSFNFE"
BASE COUNT         1594 a         1239 c         1420 g         1584 t
ORIGIN      
        1 gaccggcagt aaggccgctc gcttccaagg cggcaaagtc gggataaacc aacgccacca
       61 gtttgttttc tctttcaacg acgaggcttt ctgctacata cgggaggttg tttaacttcg
      121 cctcaatctc ttccggatag atgttctgtc cgcttggccc gaggatcatg gttttgatgc
      181 gccctttaat aaagagcctt ctgcctctca taatgccgga atcgcccgtt ttcagccatc
      241 cgtctttggt gaatgcggct tttgtggctt ccgggttttt gtagtaaccc ttcatcacat
      301 tttctccacg aacctgaatc tctcccggta cattttccgg atcgggagaa tcaattctcg
      361 cttccatgat gcccgccaga acagaaccac aggaggtggg aacaaattca tagtggtggt
      421 cgtaagagat cagtggggca cattctgtca ttccgtaacc tacggtaaag ggaaatttta
      481 ttttgtagaa aaacgcttca atatcctttc ccaaagctgc tccgccaatg attacctccc
      541 tgaaatttcc acccagggaa tcgatgagcg attttcttat tttgctgtag atcatgttgt
      601 ttattcccgg gatttggagc agtgttttca tgatgggctt gtcgacaatc ggaagaatcc
      661 tctttttgta tattttttcg aaaataagtg gaacagtaat aatgatgttc ggcttgattt
      721 cctgaaacgc cttaataaga ttttgcggcg aaggaataat atcaaggagc gtaacgtgaa
      781 cacctgtaga aagggcatat agaaaatcga atgcgcaccc gtaagcatga gccattggca
      841 ggaacgccaa atccctttca cctgggaaaa gcagctcaag ggtatgtgca taggtgatgt
      901 tcccggcaaa attattcgca gtgagcatga cccctttgct aaacccggtg gttcccgatg
      961 tgtagttcag acagataaca tcatcgttgt ccacgtcagc ataaacaaca tcttccgggt
     1021 gaaacccggc ggggtatttt tttgcaaaaa gagcatctat tttgccggta agatttcttg
     1081 tttttttatt ttcgctttga agcaagttaa atgatggaag cgaaaaaaca ggaagcttaa
     1141 tattgccttt gtccaggttc tcccaaatat tttcgttaat aaagatacat ttcgattcac
     1201 tgtgagcaat gatatgctcc atgctctcgg gattgaattc gtgcaaaata ggtacgatga
     1261 cagctccata agtgaaagta gccagaaaga taaccgacca gctggtgtgg ttctttccga
     1321 tcagcgcaac cttatctcct tcttctattc caagctctgt aaacaggata tgaatccgtg
     1381 caattttttc cgctaattgg ccgtaggata aagtctcctg atctttgtag ttggacagcg
     1441 caggtaattc ccagttcgtc ttaaaacttt cttcgtaaat ctttatgaag ttctgttgta
     1501 tcattgtttt tgcacaaata tttaagttgt gtgcaaaaat aatacgaatt tatagattga
     1561 caaagaaata ctaccgctaa attgaggcaa attttgtatt tttgcggttt ggaaacgagg
     1621 ctttttatat aaatattcaa tattcgcgtg tgttacgata acagataaat cgtttaaagt
     1681 tgtacattgg caataaacac gagcgaaact taaacatttc tattcatgat cgatcaaact
     1741 gtttttgaaa ataaaacggc tgcatattat acattgggtt gcaaactgaa ttttgcggaa
     1801 acgtctgcta tcggcaagca attgcttcat gccggagtat tgcgtgcccg gaaagggcag
     1861 aaggctgata tttgtgtcat aaatacctgt tccgttacgg aactggccga caagaaaggg
     1921 cgtcaggcca ttcggagaat gattcaaagc catcccgacg catttgtagt ggttaccggt
     1981 tgttacgccc agctcaaacc cgatgaagtt gcctccatcg acggtgtgga tttggtgttg
     2041 gggtcggaac agaagctgga tgttatccgt tatctggatg atttgaagaa aaaagacaag
     2101 ggagaagttg ttacgtctaa aacgcataga atcaggactt ttgttccctc ttgctctgcc
     2161 gacgaccgca cgcggtattt cttgaaggta caggacggat gtgattactt ctgttcattt
     2221 tgcaccatcc cttacgctgg gggaaggagc cgcaacggct ccattgccga tctggttaaa
     2281 caggcacagg atgtggtggt acagggagga aaggagatcg tgctcaccgg ggttaatatc
     2341 ggagacttcg gacatactac cggtgaaaca tttttcgatt tgataaaagc cttggatgaa
     2401 gtggaaggca tcgaacggtt tcgcatctca tccatcgaac ccaacctgct gacggatgag
     2461 atcatcgatt ttgtggcggg ctcaaaacgg ttcgcacccc atttccacac tccgctgcaa
     2521 gctggcagcg atgccgtttt gaaactgatg aagagaaaat acgacacggc attgttccgt
     2581 cgcaaggtgg aaagaataaa atctgccatg ccgcacgcgt tcatcggtgt ggatgtgatt
     2641 gtggggcttc gcggtgaaac ggacgcgtat ttcgaggaag gccgggattt tattgagtcg
     2701 atcgacttct cacagctgca cgtctttact tattccgaac gttccggaac gcaggccctg
     2761 gaaattgggc acgtcgtaga cccgcgcacc aaacacaacc ggagcaagga actgcacgac
     2821 atctccgacc gtaagttgcg tagtttctac gaatcacaaa aggacggttt cagaaaagtg
     2881 cttttcgaac acacgcgaca cggaaatagg atgcacggat tcactgagaa ttacgtgagg
     2941 ttgtatgcgg attataaccc gttgctcgta aataagataa ccggtgtgaa aataggagag
     3001 tataacgaag acgaaatggc tatgaaggct gtttttggag aataatacgc aaaaggatga
     3061 ccgggaaaaa cagaaaagga atcgggtttt acccactcta cggcgcaacg taccttatgg
     3121 caatgcttcc gttccgggta ttgtatatcc tttccgatgt tctctatata gtagtgtatt
     3181 atctggtcgg ttaccggaga aagattgtcc gaaaaaacct tctgcgttcc tttcctcgaa
     3241 agaaagagga agagatactg cagatcgaga agaaatttta ccgacacctg tgtgattatt
     3301 ttgtggaaac tgtaaaaacc ttgcgtatct ccgaaaaaga aattcgcaag cgcatggttt
     3361 ttaagaaccc tgagattgtc aaccggctca ccaaagacgg gaatacctgc ctattaagct
     3421 tggggcatta cggaaattgg gagtgggtgc cctcaatagc gttgtatctg ttgcccggag
     3481 tagagccggg acaggtgtac aagaccctga agtcaaaagc ttttgatgag ctttttctga
     3541 agatccgctc ccggttcaat cctctatcca ttgagatgaa gacggcctac cgaaccatca
     3601 ttcgcaagag aaatgagggt caggcgatgg ttatcggatt tttaacggat caacggccgc
     3661 gaaaacacaa caacgaatac tgggctacat ttttaaatca ggatacgttg ttgcagaccg
     3721 gtatggaacg aattgcccgc caactagggt tagcagtggt ctacctcgac ataaaaaagg
     3781 tggaacgagg gcattatgtg gggaattttt cggtaatcac ggcagatgca agcgccgagg
     3841 aagaatttac tgtaacggag aaatataccc ggaaactgga agaaacgata ttgaacgatc
     3901 cggcatatta tttatggtcg cataataaat ggtcccggtc gaaaaagcaa gaagcatgaa
     3961 gacatcggta gtgatattga actggaacgg acgcaaatta ctggagcggt tcctgccggt
     4021 ggtgttgcaa cacacgttgg atcaccaaac cgaagtggtg gttgccgaca atggctcttc
     4081 cgacgattcg gttgattttc taaacacccg tttcccgcag gtgccactca ttgtcctcga
     4141 taagaattac ggctttgcgg aagggtataa caaggcattg cggcaggttg acgcggaata
     4201 cgtggtattg ctgaattccg acgtagaaac taccgaaaac tggctttcaa ccatgatcag
     4261 ctacctggat gagcaccccg gtgtctctgc cctccaaccc aagatcctgg cacaaagaaa
     4321 caagaccggg tttgaatatg ccggcgcttg cggcggtttt ctggacaggt tcggttatcc
     4381 tttttgccgg ggaaggatcc tcgacaacgt ggagcaggat aggggccagt acgatacggt
     4441 aatcccggtg ttttgggcca ccggagcctg cctgtgtatc cgccggaaag attatttcga
     4501 agcaggtggc ctcgatgcgc ggtttttcgc gcacatggaa gagatcgatc tttgctggcg
     4561 gctgaatgcg cgtggaaaaa gaatcgaatg cctgccgcaa tcggtggttt atcatgtggg
     4621 tgctgcatcg ctggagaaag agaacccgag aaaaacctac cttaatttcc ggaacaactt
     4681 gttgatgttg tacaagaatg cccgcataag gagcctgctt gttgttttta ccgtccggtt
     4741 gattttagat gcgttggctt ttttgcattt attatctatg ggaaagttcg acaatgctaa
     4801 atcggttatt gaagcacaca gggacttctt caggatgatg cccggtttcc ggcacgatcg
     4861 ccgcgaaaat ttaaaacaaa gattggttac cgatattcct acaaagttca aaggcagtat
     4921 cttgtggaat tattacgtga agagaaaaaa aacgtatagc gacttaccac tcactcctgt
     4981 cactcaaaat taaaactcag tacgatcatc cgttgcgtgg ctctggccag tgaaagcgga
     5041 tattttatca attccttgtc ggtcaggtcg gatcggtcct tttcgagcat gtcgttcagt
     5101 ccgaaacaga atttgagctc ggggcaaagg ttgaagtaag gcaaataaaa atcgcaacct
     5161 ataccgattt caactccgta atcgtagggt ttcagtaaga ttggttgatt ttttcgcgag
     5221 gccagctcga tactaccgta ggttcccgcc acaaggtaag ggcgataatt gttaatgcgg
     5281 gtggacgcca gttttacctg caggggcagg gtaacataat tgttccttaa gcctgtttta
     5341 aacgtttcgc ccgaaacctg ttccttaaat acaagattct tgcttcccag atgcaatgtg
     5401 gggattgccc gcaggttcat aaagcggttc aggtacaaat cggtaatgat tcccacgcta
     5461 aatcccagtg aataggacgg gatttctgaa aaccatacct cgccgttttc atccgtgtgt
     5521 cccgattgtg tcagcatcag gtcttgcgta tgcaccccta tcgtaaatcc caggtgaaag
     5581 atcttatggt cggcgtatgg cctgttctgc agtttctgtt gttgcccgta tgttgggtca
     5641 gacaaggccg aaagcacaat aaccagaagg aatatctttc tcattacagt ttaaacgatt
     5701 ttaaagcaag gttattataa acggaatgaa aaaaatctcc ctgggattgt gtcttattta
     5761 gaaatcgtaa aaatagcacc tcgttagtaa cgataaagat atagataatc agttagatag
     5821 attctttttc tgatttg
//
LOCUS       DAOSDR010000199         5831 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrimonas sp. isolate AMR_MDS_1826
            NODE_4894_length_5831_cov_4.375866, whole genome shotgun sequence.
ACCESSION   DAOSDR010000199 DAOSDR010000000
VERSION     DAOSDR010000199.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863355
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrimonas sp. (wastewater metagenome)
  ORGANISM  Petrimonas sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Dysgonomonadaceae; Petrimonas.
REFERENCE   1  (bases 1 to 5831)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5831)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:10:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,720
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 35
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5831
                     /organism="Petrimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4894_length_5831_cov_4.375866"
                     /isolate="AMR_MDS_1826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2023866"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(59..2284)
                     /locus_tag="PLI30_08560"
     CDS             complement(59..2284)
                     /locus_tag="PLI30_08560"
                     /inference="COORDINATES: protein motif:HMM:NF024579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI79641.1"
                     /translation="MNLNEATKIHADILAFIESYRLKDAFDSLKSWAANLQNWIAAEK
                     ISELETNYKYMIHYLVEGNKDPEQQKIYQRLVRDIYLLADDLLEQWQTRNSSSVFFER
                     VRMANVRQPLSIEEYQDIIIRQIDTFSVIGLLPDEDERQTRTRQNTVKHEHTIQDLFN
                     AVFSSSRTNEEQVKAYREFLGHTGIPVPVKCMLISALTMNVLQRFDRRKVELLFDSCS
                     HAESEISLRAIVGIVAVLRQYKNRWKYYPEITGRLSVMADDAVFARRLMTVIIQFIQA
                     HETEKITKKLTEEIIPEMMKLSPMIGKKINLEEWMGESGLDEKNPEWQKILDEAGLTD
                     KLQEFTDMQMGGADVFHSTFSNLKNYPFFNEMSNWFLPFDKQHSSLQPFFSDKPESDS
                     LLSAMLNTSMICNSDKYSFCFSIMVMPGQYRDMMAAQLGAENEELKKMAEEELVLNPY
                     QKEETICKQYVQDLYRFFKLYPRKTDFVDIFAAPLDYHRLKPFLPIVAEAKNLERIAL
                     YYFEKNNFQEAGETYLMLAKKGAPTSETWQKIGYCYQMQGKVKEALEAYLKADLMDEN
                     NPWVLRRMSHCYRLLKEPESALQHYRRLEQLKPDDLNIQLNIGHCYLELDEYAEALNY
                     YFKVDLLSSDNTRAWRSIAWCAFLSRKFDVAQRYYSQIIENKPNTHDYLNAGHVEFCL
                     SNTKKAVEMYIQAVKSAGSFPVFKSLFDEDLDELREAGIDLEILPVILDKVRYEVYEK
                     K"
     gene            complement(2281..3183)
                     /locus_tag="PLI30_08565"
     CDS             complement(2281..3183)
                     /locus_tag="PLI30_08565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI79642.1"
                     /translation="MEFVDILLKRIEGENELKRPVNALICFSKVKTGKALGALMNKMV
                     RFRPDKSSVTLLNLIDAEQAKHIQDENTYKSELFSDIIQLSEANKLSVRTFVKQSENY
                     VEDILRTAEEYNCNLVLLGIGSSVFNSSLWHKYLKLREENNLPEAALQQLDEKAASSL
                     NNVSTLLARNKLSTGIFVENNYSDTNQLFVPILYKEDAYSFPYFYQMAKNPDVTVTVW
                     DAIGLMESDQKFQKLFQFIAKKTDGRVKLWDNNKKIELNFIQQQDLMIIGFNGWEKLI
                     GSPLSWTHCLPSVLIIKDNKQTLI"
     gene            complement(3231..4073)
                     /locus_tag="PLI30_08570"
     CDS             complement(3231..4073)
                     /locus_tag="PLI30_08570"
                     /inference="COORDINATES: protein motif:HMM:NF027594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4922 domain-containing protein"
                     /protein_id="HOI79643.1"
                     /translation="MQLQRKIDQLFIEQQRDWEQLRAGTEQLDHINVKEFNWGNELKV
                     DIQFNPSRIESVSAKVDKSNIEKRPCFLCEKNRPKVQSGIPFLDKYIILLNPFPILRN
                     HLTITLHSHVPQRIRKKTGDMLTLAELLPDYIVFYNGPKCGASAPDHFHLQAGLKSPI
                     LMAGENELRSCLVIESTSKSEVEERFEDVYQYLHSRQPDEEEPLFNLIAFVENNRYVL
                     HIFPRKAHRPRQFYEEGKRQLLVSPGAIDMAGLIITVREEDFNKIREEEIVDIYSQVS
                     MPII"
     gene            complement(4109..4807)
                     /locus_tag="PLI30_08575"
     CDS             complement(4109..4807)
                     /locus_tag="PLI30_08575"
                     /inference="COORDINATES: protein motif:HMM:NF027591.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4919 domain-containing protein"
                     /protein_id="HOI79644.1"
                     /translation="MKKLTIMAVLAFCSITLFAQYDGPTAPDYKLIERNINNSSSNFN
                     YSNLMERYKLGDSTMTVDEQRHLYFGYVFQPSYNPADTSQYNARMATVLNKQHFSDRD
                     YDDVLQFADALLAEDPFNMRALNAKLLVYAQKNNVDAYKKTAQQRNIVQRAIISSGDG
                     MLKSTPYYVIKVSHEYDLLGFLGFKFGGQDKIEKNCDCNSLTLAPNRFGVDKMYFNIS
                     PTFDYVKRKGSGKI"
     gene            complement(4949..5830)
                     /gene="fabD"
                     /locus_tag="PLI30_08580"
     CDS             complement(4949..5830)
                     /gene="fabD"
                     /locus_tag="PLI30_08580"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019539726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HOI79645.1"
                     /translation="MKKAYVFPGQGAQFVGMGKELYETSPLAKEMFEKANEVLGFRIT
                     DLMFGGTDEDLRQTKVTQPAIFLHSVILAKTMGGEFSPSMTAGHSLGEFSALVATGAL
                     SFEDGLKLVYKRALAMQKACEKNPSTMAAILALSDDKVEEICAGIDEVVVPANYNCPG
                     QIVISGSLKGIEIACEKMKEAGAKRALPLKVGGAFHSPLMDPAKKELSEAIAATSFSR
                     PCCPVYQNVSTIGETDPEVIKANLVAQLTAPVKWTQSIQNMIADGASEFMELGPGNVL
                     QGLVSKIDKNVTVSGKQ"
BASE COUNT         1482 a         1264 c         1288 g         1797 t
ORIGIN      
        1 tagccgccgt cctttatcag aaaagccttc aggagaaatc cggaaggctt tttttatttt
       61 actttttctc atatacctca tacctcactt tgtccaagat gacgggtaaa atctccagat
      121 caattccagc ttcacgaagt tcatccagat cctcgtcgaa cagcgattta aaaacgggga
      181 agctacctgc tgattttaca gcttggatat acatctcaac ggcttttttg gtattggaga
      241 gacagaactc tacgtgtccg gcattcaggt agtcgtgagt attgggtttg ttttcgatga
      301 tttgggaata gtaccgctgt gcaacgtcga atttacgaga taagaatgca caccaggcta
      361 tggaacgcca tgcgcgggta ttgtcggaac tcagtaaatc tactttaaag taatagttca
      421 gtgcttcggc gtattcgtca agttccaggt aacagtggcc gatattgagc tgtatattca
      481 ggtcatcggg tttgagttgt tccagccgac gataatgttg taatgcgctt tcgggttctt
      541 tcaataagcg atagcaatga gacatgcgac gcagaaccca ggggttgttt tcatccataa
      601 ggtcagcttt cagataggct tccagtgctt cttttacttt accctgcatc tggtaacaat
      661 agccaatttt ctgccaggtt tcgcttgtag gggctccttt ttttgccagc atcagatacg
      721 tttcgcctgc ttcctggaag ttgtttttct cgaaataata caaggctata cgttcgagat
      781 tctttgcttc cgcaacgata ggaaggaacg gtttcagccg atgatagtca agaggggctg
      841 cgaaaatatc gacaaaatcg gttttacgcg gataaagctt gaaaaaacgg tataaatcct
      901 ggacatattg tttgcaaata gtttcttctt tttgatacgg attgagtacg agttcctctt
      961 ctgccatttt tttcaattcc tcgttttccg ctcctagttg tgctgccatc atgtcgcggt
     1021 attgtccggg cataaccatt atgctgaagc agaatgagta tttatcagag ttgcaaatca
     1081 ttgaagtgtt gagcatagcg gaaagcaggc tgtcactctc cggtttgtcc gaaaagaaag
     1141 gttgcaagga actgtgttgc ttgtcgaacg gcagaaacca gttgctcatc tcgttgaaga
     1201 acgggtaatt tttaaggttg gagaaagtgg agtggaacac atctgcaccg cccatctgca
     1261 tgtcggtaaa ctcctgcagt ttatccgtta atccggcttc atccagaatt ttttgccact
     1321 cgggattctt ttcgtccaag ccgctttcgc ccatccactc ttccaggttt attttcttcc
     1381 cgatcatcgg gctgagtttc atcatttcgg gaatgatttc ctccgtaagt tttttagtga
     1441 ttttttcggt ttcgtgtgcc tgaataaact ggatgataac agtcatgaga cggcgcgcga
     1501 aaaccgcatc atctgccatt accgacaacc tgccggtgat ttcgggataa tacttccacc
     1561 ggtttttgta ttgtctgagc acggctacaa tacctactat agcccgcaac gagatttcgg
     1621 attcggcgtg gctgcagcta tcgaataata gctccacttt ccgccggtcg aaacgttgca
     1681 gaacgttcat cgtaagggcg gagatcagca tgcatttgac cggaacggga ataccggtgt
     1741 ggcctagaaa ttcgcggtaa gcctttacct gttcttcgtt tgttcgtgac gaagaaaaaa
     1801 ctgcgttaaa gagatcctgt atggtatgct catgttttac agtattttgt ctggttcggg
     1861 tctgcctttc atcttcgtcg ggcaaaagac cgatgactga aaaagtatcg atctggcgga
     1921 tgataatgtc ctgatattct tctatggata atggttgacg tacattagcc atccttactc
     1981 tctcgaaaaa aacggaagaa ctgttgcggg tttgccactg ttccagcaaa tcatcggcca
     2041 gtaagtatat gtcgcggacc aggcgctggt aaattttttg ttgttcggga tccttgttac
     2101 cttcaacaag gtagtggatc atatacttgt agttggtttc aagttccgat attttctccg
     2161 ccgcaatcca gttttgcaga ttggcagccc aagattttaa gctatcgaaa gcgtccttca
     2221 accggtaact ttcgataaaa gccagaatat cggcatgtat ttttgtcgct tcattcaagt
     2281 tcatatcagt gtctgtttat tatctttgat aatcaagacc gaaggaagac agtgagtcca
     2341 cgaaagtggt gagccgataa gcttttccca gccgttgaat ccgatgatca tcaggtcttg
     2401 ctgttggatg aagttgagtt caatcttttt gttattgtcc cacaacttta cccgcccatc
     2461 ggttttcttt gcaataaact gaaaaagttt ctggaacttt tggtcggatt ccataagtcc
     2521 gatagcatcc cacacggtta ccgtcacatc cggatttttt gccatttgat aaaaataggg
     2581 aaacgaataa gcatcttcct tatagaggat gggcacgaaa agctgattgg tatccgaata
     2641 gttgttctca acaaatattc cggttgaaag cttattgcgg gcaagtagtg tggatacatt
     2701 gttcaacgag gaagctgctt tttcgtcgag ttgctgaaga gcagcttccg gaaggttgtt
     2761 ttcctctctt aatttaaggt atttatgcca aagtgaggag ttgaagacag aacttcctat
     2821 ccccaacaac accaggttgc agttatattc ctcggcagta cgcaagatat cttccacata
     2881 gttttccgac tgtttgacaa atgtgcggac agaaagtttg tttgcctcgc tcagttggat
     2941 gatatcggaa aacaattcgg atttatacgt gttctcgtcc tggatgtgtt ttgcctgttc
     3001 ggcgtcaatt aaatttaaca acgtgacgga tgatttgtcg ggcctgaagc ggaccatttt
     3061 attcatgagt gctcccaggg cctttcccgt cttcactttt gaaaaacaga taagggcatt
     3121 tactggccgt tttaattcat tttctccttc gatccgtttt aaaagaatat ctacaaattc
     3181 cataccaatg tctttttaag ttgagagcaa agaacaaaga ttaagatagg ttatattatc
     3241 ggcatcgaaa cctgggagta aatgtcaact atctcttctt ctctgatttt attaaaatct
     3301 tcttcacgta ccgtgatgat cagtccggcc atatcaatgg cgcccgggct gacaagcagc
     3361 tgccgtttgc cttcttcata aaattgcctg ggacggtgag ccttacgagg aaaaatatgc
     3421 agcacgtagc ggttattttc aacaaatgca atgagattga acaaaggttc ttcctcatcg
     3481 ggctgtcggc tgtgcaggta ttgatacaca tcttcgaaac gttcttccac ctctgatttc
     3541 gaagtgcttt caataaccag acaggatcga agttcgtttt ctccggccat cagtatggga
     3601 gactttaacc ctgcctgcaa gtgaaagtga tcgggtgccg atgccccgca ctttggcccg
     3661 ttatagaaga caatgtagtc tggcagaagc tcggcaagcg tcagcatatc gcctgttttt
     3721 ttacggatac gctgtggaac gtgcgagtgt agtgtaatgg tcaggtggtt acgtaagata
     3781 ggaaagggat tgaggagaat gatgtactta tcgagaaacg ggattcccga ctgaactttt
     3841 ggacggtttt tctcgcataa gaaacagggc cgtttttcga tattgctctt gtccacctta
     3901 gcagatacag attcaatgcg agatggattg aactggatgt ccacttttag ttcgttcccc
     3961 caattgaatt cttttacgtt tatgtgatca agctgttctg tgccagctct cagttgttcc
     4021 caatcccttt gctgttcaat aaaaagttga tctatttttc gttgaagttg catctttcgg
     4081 tcatttgaat aacaaagaga aggataattt aaatcttccc gcttcctttt cgtttgacat
     4141 aatcgaaagt aggcgaaata ttgaagtaca ttttatccac cccaaaccgg ttgggggcaa
     4201 gcgtcaagga gttgcagtcg cagtttttct cgattttatc ctggcctccg aacttaaaac
     4261 ctaaaaagcc caacaggtcg tattcgtgag aaacctttat cacgtaataa ggggtcgact
     4321 tcaacattcc atcgcccgag ctgataatag ctcgttgtac aatgtttctt tgttgagctg
     4381 tttttttgta tgcatccaca ttattttttt gcgcataaac caataatttg gcgtttaagg
     4441 cccgcatgtt aaacggatct tctgccagta aagcatcggc aaactgcaat acgtcatcat
     4501 aatcccggtc tgaaaaatgc tgtttgttaa gaacggtggc catccgggca ttgtactgcg
     4561 atgtatcagc cggattgtac gatggttgaa aaacataacc aaaatacaaa tgacgctgtt
     4621 catccactgt cattgtagag tctcccagtt tatagcgttc catcaaattg gaataattga
     4681 agttggaaga actgttgttt atgttccgtt caattaattt ataatcgggt gcggttgggc
     4741 cgtcatattg tgcaaaaagt gttatgctac aaaaggcaag cacagccatg atcgttaatt
     4801 ttttcatttt tttggttttt tgatgaatat gcgaacaaat gtattttgta ttttcgattt
     4861 taacaaatta gtttcactaa caaaacttaa atagacatca gatgtcagac cgcttccctt
     4921 ttaaactctt ggaggtctgt cgtcactttt attgtttccc cgaaacagtt acgtttttat
     4981 cgatcttcga taccaagcct tgcagtacgt ttcccgggcc aagctccata aactcggagg
     5041 cgccgtcggc aatcatgttc tgaatcgatt gagtccattt tacaggagca gtcagctgtg
     5101 ccactagatt tgcttttata acttccgggt cggtctctcc gattgtagaa acattctgat
     5161 aaaccggaca acaaggcctg gagaaggagg tcgcagcaat tgcttcggat aattcttttt
     5221 tagcgggatc catgagcggt gagtggaatg ccccacccac ttttagcggg agagcccttt
     5281 tggccccggc ttctttcatt ttctcgcaag cgatttcaat ccctttcagc gatccggaaa
     5341 taacaatctg tcccggacaa ttgtaattgg caggaacaac tacctcgtcg atcccggcgc
     5401 aaatttcttc gaccttatca tcggacaggg ccagtatggc agccatggtc gaagggttct
     5461 tttcgcatgc tttctgcatg gccaacgcac gtttataaac cagttttaat ccatcttcga
     5521 acgacaaggc tccggtggcc accaacgctg aaaattctcc caatgagtgg cctgcagtca
     5581 tactgggact gaactcgcct cccatagttt tagcgagaat cacggaatga agaaaaatag
     5641 ccggttgggt gacttttgtc tgacgaagat cttcgtctgt ccctccgaac attaaatccg
     5701 taatacggaa acccaaaact tcgttggctt tctcgaacat ttcttttgcc aacggtgatg
     5761 tctcgtacaa ctcttttccc ataccgacaa actgtgctcc ttgtccggga aatacatatg
     5821 cttttttcat a
//
LOCUS       DAOSDR010000200         5818 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrimonas sp. isolate AMR_MDS_1826
            NODE_4914_length_5818_cov_4.970675, whole genome shotgun sequence.
ACCESSION   DAOSDR010000200 DAOSDR010000000
VERSION     DAOSDR010000200.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863355
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrimonas sp. (wastewater metagenome)
  ORGANISM  Petrimonas sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Dysgonomonadaceae; Petrimonas.
REFERENCE   1  (bases 1 to 5818)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5818)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:10:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,720
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 35
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5818
                     /organism="Petrimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4914_length_5818_cov_4.970675"
                     /isolate="AMR_MDS_1826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2023866"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            324..1868
                     /gene="istA"
                     /locus_tag="PLI30_08585"
     CDS             324..1868
                     /gene="istA"
                     /locus_tag="PLI30_08585"
                     /inference="COORDINATES: protein motif:HMM:NF033546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HOI79646.1"
                     /translation="MEDFKMYSKIQQEKNQGFSRGTVARHLGLSWRTVDRYWDMSADS
                     YKALNKKHHSSILDKHEITIVGWLEEFPDLSAAQVKDWIHEHYDEMFPERTVRNYVKT
                     LREKHCIEKPVKSREYGCIPELPPGQQLQADFGEYWCIRKGKSRIKLYLAAFILAHSR
                     YKYVMWQTKPFTAVDFVRTLESCFEALGGTTHELVIDQDTLMTVSENNGDIIHTYEFE
                     RCKNRHGFTVWLCRKADPESKGMVESCVKFIKYNFAKNRYFYDINQWNNDCDAWLKRT
                     GNGKVHSETKKIPAEIFELEKKHLKPVILFNDNIPSNNSLTTPVRKNNTIRYNASRYS
                     VPIGTYTLFKEVSVQEIDDHLEIYDPTGKLIASHELAVSPGELKRNNNHVRDNSEKIK
                     NLMEQAKKALGDTPEANEFLSIIHKRRGRYIRDQLHLILKVADSYSYDVLQLTLKACC
                     ECNLNSANDFRDFAEHIFRQKAVDEIISPQIRPLPGSLSSTDRDISVNQPNPGSYMNL
                     INKGGQ"
     gene            1870..2283
                     /locus_tag="PLI30_08590"
     CDS             1870..2283
                     /locus_tag="PLI30_08590"
                     /inference="COORDINATES: protein motif:HMM:NF013828.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOI79647.1"
                     /translation="MGNNLKQLKNQLKELKLKHLAAQFDDFMISACNGEKPNTQFLMD
                     ALEIELSERRRLSMEKRLKYAKFPVFDKPFDLNGFDFGKRQGVSKRQILELSSNLIWI
                     DKAYNVLFLGGSGLGKSFLACYLGYKAAEAGYCFS"
     gene            3029..4375
                     /locus_tag="PLI30_08595"
     CDS             3029..4375
                     /locus_tag="PLI30_08595"
                     /inference="COORDINATES: protein motif:HMM:NF012870.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
                     /protein_id="HOI79648.1"
                     /translation="MRNQKKAEQIAEQRIQILSPLLEEGLDAAKTREIKVRICEQTGM
                     SERTLRRYLAQYRNSGFVGLKPKGRGRKNTEEAIPSHLLEQAILLRRQVPGRSVSQII
                     QILEWEGLAEPGKIKRSTLQEKLAERGYSSRQMKLYTNTGVAARRYQQAYRNKLWHSD
                     IKYGPYLPIGKGGMKKQVYLVTFIDDATRMILHGEFYPMLDQVIVEDCFRKAIMKHGV
                     PEAVFFDNGSQYRTKWMNRACAKMGIRLLYAKPYSPESTGKPERFNQVVDSFLAEVAL
                     EKPQTLDKLNEFFQIWVEECYQNKPHSALKDGNTPLTAYRSDKKPQKFLDPDVITNAF
                     LHCEQRKVDKAGCISFEGKKYEVGISFIGCTVDVVFDPADTNELTIEYEGHSPWKAKQ
                     LVIGERVGKRPKLPEHLTKVGTDSSRLLKAATIKNQQRREQQTPAISFRNVIKAGE"
     gene            4377..5177
                     /locus_tag="PLI30_08600"
     CDS             4377..5177
                     /locus_tag="PLI30_08600"
                     /inference="COORDINATES: protein motif:HMM:NF024793.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOI79649.1"
                     /translation="MFEQFYGFIRSPFTRDIPTDELYKSVMMDEMVGRLLYAAERQLF
                     TVVTGDCGVGKTTMLRCFINNLNASRFSALYLADSKLTPRHFYKGLLEQLGCEAKFYR
                     GDAKRQLHKEIELMRGIHNLLPVVIVDEAHLLDKEMLEEVRFLLNFKMDSKSPMALTL
                     VGQSELWDRLKLQSYAAIRQRIDLQCKLGYYDRSQAGEYVKRHLKYAGNDNEIFSDAA
                     VDEIYRFSSGSARLINKLCTHCLLYGAQNGKRIIDDHMVKLVIQGELS"
     gene            5174..5494
                     /locus_tag="PLI30_08605"
     CDS             5174..5494
                     /locus_tag="PLI30_08605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI79650.1"
                     /translation="MIEFRCTYQWDENDKDDWYGAIKQIINYGSHYEVFISSRSSLRV
                     LIGKSSSGLFACLPDFRVGCHLSTLDDTFYNSEKLIYALNNPVDGTTVACALNAITSV
                     LRFR"
BASE COUNT         1822 a         1129 c         1301 g         1566 t
ORIGIN      
        1 tcttgcgtaa aaaatttatc cattaatctc ttcctcccta tcctctggca cttttctaaa
       61 ggcaccactt ccctcaaggt ttgaaagaag tgtctttctt atggccttat actcctttcc
      121 gttcatccca agtctaatgg tagcggcaat aaaaagtgca tagatttcgg caatacaaag
      181 taagtaaaaa cggcaaccta aagtaagtaa atccggcaat caaatgtcac atatttcggc
      241 aacggaagta agtgatttgt cgaaatttac ctcctgaaag tgataagata ttttcatctt
      301 acctcgatag gagggtcaaa tgattggagg attttaaaat gtattcaaaa attcaacagg
      361 agaaaaacca aggtttttct cgaggaactg tagctcgcca tttaggcttg tcatggcgaa
      421 cggttgatcg gtattgggac atgtcagccg atagttacaa agctttaaac aaaaagcacc
      481 actccagcat cctggacaag catgaaataa ccatcgtagg gtggctggag gagtttcctg
      541 accttagtgc agcacaggtg aaagattgga ttcacgaaca ctacgacgag atgtttccag
      601 agcgaacagt tagaaactat gtgaaaactc ttagggaaaa gcactgtatt gaaaagcctg
      661 tcaaatcccg tgaatatggt tgtatacctg aattgccacc tggacagcaa ctacaggcag
      721 actttggaga atactggtgt atcagaaagg gaaagtcccg cattaaactc taccttgccg
      781 cttttatcct ggcacattcc cgttacaagt acgttatgtg gcaaacaaaa cctttcactg
      841 ctgtagattt cgtccgaacc cttgagagct gttttgaggc tcttggagga actacacacg
      901 aattggtcat tgatcaggat actttgatga cagtcagcga aaacaatggt gatattattc
      961 atacttacga atttgaacgt tgtaaaaaca ggcatggatt cactgtatgg ctttgtcgta
     1021 aagcagatcc agaatcaaag ggaatggtag agtcttgcgt aaagtttatt aaatataatt
     1081 ttgccaagaa cagatacttc tatgatataa atcagtggaa caatgactgt gatgcatggc
     1141 ttaaacgtac aggcaacggc aaagtccatt ctgagaccaa aaagataccg gccgagattt
     1201 ttgaattgga aaagaaacac ctcaagccgg tcatcctttt taatgacaat attccaagta
     1261 ataatagttt aacaactccg gtacgtaaaa acaataccat tcgttacaac gccagccgtt
     1321 attcagtccc cataggcaca tacactcttt ttaaggaagt gtctgtacag gagatagatg
     1381 accaccttga aatatatgat cctactggaa agttaattgc ctctcacgaa cttgcagtaa
     1441 gccctggtga actaaaaaga aataataatc atgtccgtga taactcagaa aaaataaaaa
     1501 acctaatgga acaggctaag aaggctcttg gtgacacacc tgaagcaaat gagtttctaa
     1561 gtattattca caaaaggagg ggacgttaca tccgggacca attgcattta atcctcaagg
     1621 ttgcagacag ctatagctat gatgtcctgc agttaacctt gaaggcttgt tgtgagtgta
     1681 atctgaattc cgcaaatgat ttcagagact ttgcagaaca tatatttcgt cagaaagctg
     1741 tagacgaaat catctcccct caaataaggc ctctcccggg ttctctgagt tccacagacc
     1801 gtgatattag cgttaatcag ccgaatccag ggtcatacat gaatctgatc aacaaagggg
     1861 gacaataaca tgggaaacaa tctcaaacaa cttaaaaacc agcttaaaga gctcaaacta
     1921 aaacaccttg ctgctcaatt tgatgatttc atgatttcag cttgtaatgg agaaaaaccc
     1981 aacactcaat ttctaatgga tgctctggag attgaacttt cagagcgccg acgactcagt
     2041 atggagaaac gccttaaata tgccaagttc ccagtctttg ataaaccttt tgacctgaat
     2101 ggttttgatt ttggtaaaag acagggagtt tcgaaaagac agattctaga gctatccagt
     2161 aatttgatat ggatagataa agcatacaat gttctttttt tgggtggttc cggcttggga
     2221 aaaagctttt tggcatgcta tttaggatat aaagcggcgg aagcaggata ttgttttagc
     2281 taatctaaaa agccacaaag ctgacggtat tgcctgtccg aaaatagatg gatataataa
     2341 ccatataagt tttaagaaag gaaaaggctt tcttgttaat agtgtcaccg cactcaacag
     2401 tagcacccaa gaaagcctcc tcaaaaacct atggttattg tatcaaaata tgagcttgtt
     2461 gaaaagacca aaaagggagt tttttgtacg gagtttggag agaataccct gcccccgttg
     2521 cagcagtctt aaaatgaaag ttatcggttc tcggaagagg aaatatatca ctttagaagg
     2581 caataaaaat atacttatca ttcgaagatt gagatgccag cattgcgggg gaatacatca
     2641 tgaactaccg gatattcttg ttccatacaa aagatatagc agtgaatgta ttgaaacagt
     2701 aatatccatg aatgagccaa tctcagttcc agccgatgat tctacacttt tgcgatggcg
     2761 taattggttt tcaaagataa gttttcactt ccttgggtgc ctgcaatcaa tctcagttca
     2821 gctatatggt tattcaaaag tagctccaac aggccaatcc gtcctccata ggatatggca
     2881 ctatgtagat agtacttatg gttggatggc aagagttgtc caaccggtcg caaatatgaa
     2941 tttttgggta catacccgtt ctgcattcat ggcctgacag ccataggata aactcatgtt
     3001 gaacaaacct taaaggaggg aattcgacat gaggaatcag aaaaaagcag aacaaattgc
     3061 ggaacaacga attcagattt tgtctccgtt gttagaagaa ggacttgatg ccgccaaaac
     3121 tcgtgaaatc aaggtgagga tatgtgaaca aacaggcatg tccgaacgta ccttgcgcag
     3181 atacctggca caataccgga attcagggtt tgtagggtta aaaccaaagg gacgtggtag
     3241 aaaaaatacc gaagaagcta tcccatctca tctgttggag caggcgattc tactgcgcag
     3301 gcaggtacca ggacgcagtg tatcacaaat cattcagatt ttagaatggg aaggtttagc
     3361 tgaaccggga aagatcaagc gaagtacgct gcaggagaaa ctggctgaga ggggttacag
     3421 ctcaaggcag atgaaattgt ataccaatac cggtgttgct gcccgcagat atcagcaagc
     3481 gtatcgcaac aagctttggc attcggatat taagtatggc ccatacttac ccattgggaa
     3541 aggtggaatg aaaaagcaag tataccttgt gacattcatc gatgatgcaa cacggatgat
     3601 acttcatggt gaattctatc caatgctgga tcaggtaatc gtagaggatt gttttcgtaa
     3661 agcgattatg aaacacggag ttcccgaggc tgtttttttt gacaacggta gccagtaccg
     3721 gactaagtgg atgaaccggg cttgtgcaaa aatgggtata cgccttctat atgcaaagcc
     3781 gtattctcca gaatccacgg ggaagccaga gcggtttaac caggttgtgg attcttttct
     3841 tgcagaagtt gctctggaaa agccacaaac attggataag ctaaacgaat tttttcaaat
     3901 ttgggttgag gaatgttacc aaaataaacc tcattctgca ttaaaagacg gcaatactcc
     3961 attgacagct taccgtagtg acaagaagcc gcaaaagttc ttagatccgg acgttatcac
     4021 caatgccttt ctccactgcg aacagcgcaa agtagataag gctggttgta ttagcttcga
     4081 aggtaagaag tatgaggtag gtatttcctt tatcggttgc acagtagatg ttgtttttga
     4141 ccccgcagac accaatgagt taacgattga atacgaaggc cactccccat ggaaagcgaa
     4201 acagctggtt atcggtgaaa gggtaggaaa acgaccaaaa ctaccggaac atcttactaa
     4261 ggttggaact gattcatccc gcttgcttaa agctgctaca ataaaaaatc aacagagaag
     4321 agaacagcaa acacctgcaa tttcctttag aaatgtcata aaggcaggtg aataaaatgt
     4381 ttgaacaatt ttatgggttt atcagatcac cctttacccg ggatattcca acggatgaac
     4441 tgtacaaatc tgtgatgatg gatgaaatgg ttgggcgact gttgtatgca gcagaacgac
     4501 aactcttcac tgttgtcact ggcgactgtg gtgttggaaa gacaacaatg cttcgttgtt
     4561 tcattaataa cttgaatgct tctcgttttt cagcattgta tttggcagat tcaaagctga
     4621 caccgaggca cttttacaag gggctgctag aacagctggg atgtgaggct aagttctatc
     4681 gtggtgatgc aaaaaggcaa ttacacaagg aaattgaact tatgcgtggg attcataatc
     4741 tgcttccagt ggttatcgta gatgaagcgc atcttttgga caaggagatg ttagaggaag
     4801 ttcgttttct actcaatttc aagatggatt cgaagagccc catggctttg actttggttg
     4861 gccaatcgga attgtgggac aggctgaagc tgcaatcata tgctgccatt cgtcagagga
     4921 ttgatctcca atgcaaattg ggctattacg atcgttcaca agctggtgag tacgtcaagc
     4981 gacatcttaa gtatgcggga aacgacaatg agatcttttc agatgctgcg gtggatgaaa
     5041 tctacaggtt ttcaagcggc tcagcccggc tcattaacaa gctttgtact cattgcctgc
     5101 tttatggtgc acaaaatggc aagcggatca tcgatgatca tatggtgaag cttgtaatac
     5161 agggggaact gtcatgatag agttccgctg tacatatcaa tgggatgaaa atgataaaga
     5221 tgattggtat ggtgccataa aacagattat taactatggc agccattatg aagtattcat
     5281 ctcgagtcgg tccagtttac gtgtattgat tggaaaatcc agctctgggc tatttgcctg
     5341 ccttccggat tttcgggtcg gatgtcattt gagtaccctg gatgatacct tttacaacag
     5401 tgaaaagctg atttatgctt taaacaatcc ggtagatggt actacagttg cctgcgcgtt
     5461 aaatgcaata acttctgttt tgagatttcg ttaagggttt gaaaggagtg cggtaaccaa
     5521 ccaaaaaggt tatccgctga ctctatatct taatactgta ttgatatgat gttcataaag
     5581 tgaaaggccg gttttcttct cccgggagga tagggcaacg ctggggagcg acccgagcgt
     5641 ccgtggaggc attaaacggt tgaaacttcc cccatttccg ggggaagaaa gccggccaca
     5701 ttttaaccgg aaatgcctaa agcgataaac ccactaaaca atccttggac acaaaatcaa
     5761 gcagttttag gacaattacc agcgtctatt tctggacact ttagcttatc agtaacaa
//
LOCUS       DAOSDR010000201         5804 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrimonas sp. isolate AMR_MDS_1826
            NODE_4929_length_5804_cov_4.366499, whole genome shotgun sequence.
ACCESSION   DAOSDR010000201 DAOSDR010000000
VERSION     DAOSDR010000201.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863355
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrimonas sp. (wastewater metagenome)
  ORGANISM  Petrimonas sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Dysgonomonadaceae; Petrimonas.
REFERENCE   1  (bases 1 to 5804)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5804)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:10:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,720
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 35
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5804
                     /organism="Petrimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4929_length_5804_cov_4.366499"
                     /isolate="AMR_MDS_1826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2023866"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1500)
                     /locus_tag="PLI30_08610"
     CDS             complement(<1..1500)
                     /locus_tag="PLI30_08610"
                     /inference="COORDINATES: protein motif:HMM:NF025957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HOI79651.1"
                     /translation="MKSIIFVVSLLCTVALPAQTQWHDPLKEEAHVIQNQGWTREIGK
                     TYQRLPDRAEKKVRKAVWNLSENSAGLAIHFYTNAAKIEVRYGVAGSHAMLHMPATGK
                     SGVDLYAMDSDGKWRVATDKYSFGDTVTYTFNNLSQSRYHKQGFEYRLFLPLYNSVKW
                     MEIGVPDSAEFKFIPVLREKPIVVYGTSIAQGGCASRPGMAWTNILSRKLDYPVVNLA
                     FSGNGPLEKEFVDLIDEIDASLYVFDCLPNMGSLSSEEVIKRTVYGVKKLREKHDAPI
                     LIVDHIGYRNDELNELSRETADRMNVASRQAFDSLVELGVKNIYHLHKDSINMPADGC
                     VDNIHPSDLGMQVYADAYEKIIRKILNMPVGKISTTRPVSQRREPYIYEWKERHAGIL
                     QNIQLQRPEKVIIGNSITHYWGGEPGRENGSQSWKKQVQPAGYLNLGYGWDRVENVLW
                     RVYHGELDDYRASEVVLMIGTNNLGSVSDEEIVEGLEFLLEQIKIRQPEA"
     gene            complement(1497..2939)
                     /locus_tag="PLI30_08615"
     CDS             complement(1497..2939)
                     /locus_tag="PLI30_08615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005634900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HOI79652.1"
                     /translation="MDISREEIQDFLNRNIFKLISEVADRMELDCYVIGGYVRDFFLY
                     RSSKDIDIVAVGKGIALAEAVASALGPRTKLSVFKNFGTAQLKYKDFEIEFVGARKES
                     YTHDSRNPVVEDGTLEDDQKRRDFTINAMAFCLNKNRFGELLDPFNGLQDLQDLIIRT
                     PLDPDITFSDDPLRMMRAVRFASQLGFDIFPETFDAVARNKERIKIISKERIADELNK
                     IILSPKPSVGFVLLEKTGLLEIILPELVALKGAETKEGVGHKDNFYHTLAVLDNISLK
                     TDNLWLRWSALLHDIAKPVTKRWDNKLGWTFHNHNYVGEKMVPRIFSRMKLPLNEKMK
                     YTQKMVSMHMRPMQLVEEEVTDSAVRRLLFDAGDDIDDLMTLCEADITSKNAEKVRRH
                     LQNFRIVRRKLTEIEEKDRVRNFQPPIDGNEIMEIFGLGQGREVGELKSFIKEAILEG
                     EIPNERHAAYDFLIRKAKETGLEPIKKTKS"
     gene            3038..3904
                     /locus_tag="PLI30_08620"
     CDS             3038..3904
                     /locus_tag="PLI30_08620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI79653.1"
                     /translation="MKGTLSNKLYPRPLSGYCFVFTAVCVFGLLLFSCSGKGKNAKTY
                     LAEAESALSAGNYSLAKLKIDSIKVLYPQSFDEINKGFGLMQRVRMAENQRNIVYCDS
                     MLAVNYQLLTKMLNDFTYVRDPQYQEFGDYVPKIYPLSSSFHQNGLRSAVSEKGQMYI
                     ESVLSGGSLKHNRIKVATKDGSFAESLPVTTDGLNYRFSTLNTTYEIVRFSGNDDNGV
                     AKFIYTFKDSPLTLSFIGSRTTSIPLPEKAKKGIAQSFELSTLLLDIENLKYEKGRSE
                     ALINYLESRKTD"
     gene            complement(3915..5534)
                     /locus_tag="PLI30_08625"
     CDS             complement(3915..5534)
                     /locus_tag="PLI30_08625"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOI79654.1"
                     /translation="MLRFLKFLTDISRGHRKALMVITLIGVLSVAVSLSFVWALKLVI
                     DVASGDATGSLLHYSLILVMLTLFRVLLNVVDVRYVNMTEVRVGNSIRQKVFANLLYT
                     RWTEASSLHSGDVLTRMIKDTDDIIKTLISAIPMLITAVLQLGGAFLILLLMDPLLAL
                     VLGIAMPVLLLFSKPYYVKMRDYSRQIKESESFITSMMEENLLNQLVVRTYERQEDEL
                     DRLGNLQRELQRKVEKKTTVSAWARFVSGLAFSGGYITAFIWGAWGIAGKTITFGTVT
                     AYLQLVNQIQRPLYDLMRLFPTLISAQAASERLSFLKKLESEEIGEKQFLAGRVSLRV
                     EDITYAYGDESRPVISGFSLDAVPGEMVAVMGETGTGKTTLIRLILALIEPQKGSISI
                     RNEERAVKVSPSTRSNFVYVPQGNTLFSGTIRSNLLIGNPDAGEEMLVEALEVASAGF
                     VLELPEGLDTVLGMGGSGVSEGQAQRIAIARSLLRPGGILLLDEATSALDIETEKTFL
                     TNLKKHIGSRTVLFITHHAEVARYCDRIYRI"
     gene            complement(5535..>5804)
                     /locus_tag="PLI30_08630"
     CDS             complement(5535..>5804)
                     /locus_tag="PLI30_08630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI79655.1"
                     /translation="LGADPGVFPFTTDTEGKFVDVVMDDVLRGGNFGFSTFRNKSFRG
                     KWDAKWHRFTYSVARTKKISGIAPRHINPLPVTKITTNLKLLFKK"
BASE COUNT         1431 a         1592 c         1287 g         1494 t
ORIGIN      
        1 ggcctccggt tgacgaatct ttatctgctc aagcaggaac tccagcccct caacaatctc
       61 ttcatcagaa actgatccca agttattggt cccgatcatc agaaccactt cactggcccg
      121 gtagtcatcg agttcgccat gatacacgcg ccaaagcaca ttttccaccc gatcccaccc
      181 gtatcccagg tttaaatacc cggcaggctg tacctgtttt ttccagcttt ggctcccgtt
      241 ctcccgtccg ggctccccac cccagtaatg ggtaatggaa tttccgataa tcactttttc
      301 cggacgctgc aactgaatgt tttgcaggat accggcatgc cgttctttcc attcgtaaat
      361 ataaggttcc cttcgctggc ttacaggcct ggtagtgctg atttttccga caggcatatt
      421 cagtatcttc cggattattt tctcgtatgc gtcggcgtag acctgcattc caaggtcact
      481 gggatggatg ttatccacac atccgtcagc aggcatgtta atggagtcct tgtgcaggtg
      541 gtaaatgttc ttcacaccca gctcaaccag cgaatcgaat gcctggcgcg atgccacatt
      601 catccggtct gccgtttccc tggacaactc gttgagttca tcgttcctat acccgatatg
      661 atccacgata agaataggcg catcgtgctt ttcccggagt tttttaactc cgtaaacggt
      721 tctttttatc acttcttcgg atgacaagct ccccatgttg ggaagacaat caaaaacgta
      781 gagcgatgcg tctatttcat ctatcaaatc tacaaattcc ttttccagcg ggccatttcc
      841 ggaaaaagcc aggttgacga ccggataatc gagctttctc gataaaatat tggtccaggc
      901 cattcccggg cgggaagcac atccaccttg tgcgatggac gtcccgtaaa ccactatcgg
      961 tttctctctg agcacgggaa taaacttgaa ttccgccgaa tcggggactc caatctccat
     1021 ccattttacc gaattgtaaa gcgggaggaa aagccggtat tcaaaaccct gcttgtggta
     1081 gcggctctga gacaggttgt tgaacgtgta ggtaacggta tcgccaaagc tgtatttatc
     1141 ggttgctacg cgccatttgc catcggaatc catcgcatag aggtcgactc ccgactttcc
     1201 ggtggccggc atgtgtagca tggcgtggct gccagccaca ccgtaccgca cttcaatttt
     1261 cgcggcgttc gtgtagaaat gaatggccag cccggctgag ttttccgata aattccaaac
     1321 cgcctttctc actttcttct ctgcgcggtc gggcaggcgc tgataggttt ttccgatctc
     1381 acgggtccaa ccctggttct ggatgacgtg tgcttcttct ttcaacggat catgccattg
     1441 ggtttgtgca ggcaaagcaa ctgtgcacaa aagactgaca acaaaaataa tacttttcat
     1501 gatttggttt tttttatcgg ttccaacccg gtttcttttg cttttcggat caggaagtca
     1561 taggccgcat gacgttcgtt gggaatctct ccctccagaa tagcttcctt gataaatgat
     1621 ttcagttcac ctacctcccg tccctgcccc aaaccgaaga tctccatgat ctcgttgccg
     1681 tcgatcggag gctggaaatt tcgcacacgg tccttttctt caatctccgt aagctttctg
     1741 cggacgatcc tgaaattctg gaggtgccgc cgtactttct cggcgttttt cgatgtgatg
     1801 tcggcttcac aaagagtcat caaatcgtct atatcgtcac cggcatcaaa caacagacgc
     1861 cgaacggcag aatccgtcac ctcctcctcc accagctgca tcgggcgcat gtgcatcgac
     1921 accattttct gtgtgtattt catcttttcg ttcaacggca atttcatgcg gctgaagatc
     1981 ctgggcacca tcttttcacc cacatagttg tggttgtgaa atgtccagcc cagcttattg
     2041 tcccagcgtt tggtaaccgg cttggcaata tcgtgcagca aagccgacca ccgcaaccag
     2101 aggttgtccg tctttaacga gatgttatcg agaacggcaa gcgtgtggta aaaattatcc
     2161 ttgtgcccca ccccttcctt ggtttcggct cccttaagcg caaccagttc tggtaaaata
     2221 atctccaaca agcccgtctt ctccagcaaa acaaagccga cagacggttt gggggaaaga
     2281 atgattttgt tcagctcatc cgcaatacgt tccttggata tgatttttat gcgttcctta
     2341 ttccgggcaa ccgcatcaaa cgtttcgggg aaaatgtcga atcccagctg cgaggcaaac
     2401 ctcacggccc gcatcatccg caacggatcg tcactgaacg tgatatcggg atcgagcgga
     2461 gtgcggataa tcaagtcctg caggtcctgc agtccgttaa acggatccag caattcgccg
     2521 aaacggtttt tattcaggca gaaagccatt gcattgatcg tgaagtcgcg gcgtttctgg
     2581 tcatcttcca gtgtaccatc ctccacgacg ggatttcggg aatcgtgggt gtaagactct
     2641 ttgcgggcac cgacaaactc gatttcaaaa tccttgtatt tcagttgtgc cgtaccaaaa
     2701 tttttaaaaa ccgacaactt cgtgcgtggc cccaacgctg aagcaacggc ctcggccaag
     2761 gcaatcccct tgccaacggc aacaatatct atgtcttttg acgagcggta taaaaagaaa
     2821 tcacgaacgt aaccgccgat cacgtagcaa tcgagctcca tccggtcggc cacttccgaa
     2881 ataagtttaa agatgtttct gtttagaaaa tcctgtatct cttcgcgaga aatatccatt
     2941 agcaaatcaa attctgttat gatttgcaaa attacaataa ttgagcgtga aaaccatttt
     3001 aacgttgaat tttatttctt atttttgtat actgattatg aaagggactc tatcaaataa
     3061 attatatccc agaccgttat ccggttattg ttttgtcttt acggctgttt gtgtttttgg
     3121 acttcttctt ttctcctgtt cagggaaagg taaaaatgcc aaaacctacc ttgccgaagc
     3181 cgaatcagcg cttagcgcag gcaactattc gctggcaaag ctcaagatcg acagcatcaa
     3241 ggtgctttat ccccaatcgt tcgacgagat aaacaagggg ttcggactga tgcagcgtgt
     3301 gcggatggca gaaaaccaaa ggaacattgt ttattgtgac tcgatgcttg cggtcaacta
     3361 ccagctgctt accaaaatgc tgaacgactt cacttacgtc agggaccccc agtatcagga
     3421 gtttggagat tatgtcccca aaatatatcc gctgagcagc tcctttcacc agaacgggct
     3481 ccgttcagcc gtaagcgaaa aaggacagat gtatatcgag agtgtgcttt cgggtggctc
     3541 gctgaagcac aaccggataa aagtggctac aaaagacggc tcctttgcgg agtcactgcc
     3601 cgtcactacc gatggactga actaccggtt cagcacgctc aacactacat acgagattgt
     3661 ccgcttctcg ggaaacgatg acaacggcgt ggctaaattc atctacacat ttaaagattc
     3721 accccttacc ctcagcttta tcggaagtag gaccacctcg attcccctgc ccgaaaaggc
     3781 aaagaaaggc attgcgcagt cgttcgagct ctccacactc ctgctcgaca tagaaaacct
     3841 caagtatgaa aaagggcgca gtgaagcact gataaactac ctggaaagca ggaaaacgga
     3901 ttaagaaact gtttctaaat tctgtaaatg cggtcgcaat acctggctac ctccgcatgg
     3961 tgggtgatga acagcaccgt cctactgcca atgtgttttt taaggttggt cagaaacgtt
     4021 ttttcggtct caatatccag agccgatgtg gcttcatcca gcagcagaat tcctccgggg
     4081 cgaagcaacg agcgtgcaat agcaatccgt tgagcctggc cttcggaaac ccccgaaccg
     4141 cccataccaa gaaccgtatc cagtccttcg ggcagctcca ggacaaaacc tgcggaagcc
     4201 acctcgagcg cttccacgag catctcctcg ccggcatccg gatttcctat cagcaggttg
     4261 ctccgaatag tgccgctgaa caacgtgttg ccttgcggaa catatacaaa attcgaacgg
     4321 gtggacgggg acaccttcac cgccctctct tcattcctta tcgagatcga ccctttctgc
     4381 ggctcaatca gggcaaggat cagcctgatc agcgtggtct tccccgtacc ggtttctccc
     4441 atgaccgcaa ccatctctcc cggtacggca tccagtgaaa atccggaaat gaccggcctg
     4501 ctttcgtctc cgtacgcgta agtgatgtcc tctacacgca acgagacacg gcctgccagg
     4561 aattgttttt ccccaatctc ctcgctctcc agctttttca ggaacgatag ccgttcactg
     4621 gccgcctggg cagaaatcag ggttgggaac aggcgcatca gatcgtacag cggacgttgt
     4681 atctgattga ccaactgcag gtaagccgta acggtaccga acgtaatcgt tttaccggca
     4741 attccccagg caccccatat aaatgcagtg atgtacccgc cgctgaatgc caatcccgag
     4801 acaaagcgtg cccaggccga taccgtggtt tttttctcca ccttccgctg cagttcccgc
     4861 tgcaggttcc ccagcctgtc caactcatcc tcctgccttt cgtacgtccg caccaccaac
     4921 tgattcagta aattttcctc catcatggaa gtgatgaagc tttcgctttc ttttatctgc
     4981 cggctgtaat ccctcatctt cacgtaatag ggtttgctga aaagaagcag aaccggcatg
     5041 gcaatgccca agacaagggc caggagcgga tccatcaaca gcagaatcag aaatgcccct
     5101 cccagctgca aaacagccgt tatcagcatc gggatcgcgg atatcaatgt ttttatgata
     5161 tcgtccgtat ccttgatcat tcgggtcagc acatcgccac tatgcagtga gctggcctcg
     5221 gtccaccttg tatagagcag gtttgcgaaa actttttgac ggatagaatt cccgaccctc
     5281 acctcggtca tgttcacgta gcgcacatcc accacgttca gcaataccct gaacaacgta
     5341 agcatgacga gaatcagtga ataatgcaac agcgagcctg tggcatctcc cgacgcgaca
     5401 tcaataacca gcttcagggc ccacacgaaa ctcagcgaca cggccacact taacactcca
     5461 atcagcgtta tcaccatcag cgctttacga tggcccctgg aaatatcggt cagaaacttc
     5521 agaaaacgta acatctattt tttgaacagc agtttcaggt tggttgttat tttagttacc
     5581 ggcaaagggt ttatgtgccg gggagcaata ccggatattt ttttcgttct cgctaccgaa
     5641 taggtgaacc tgtgccattt tgcatcccat ttcccccgga aagacttgtt cctgaaggta
     5701 gaaaacccga aattacctcc cctcaacacg tcatccatca ccacatccac aaattttcct
     5761 tccgtatcgg tagtaaacgg gaaaacaccc ggatctgcac ccag
//
LOCUS       DAOSDR010000202         5791 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrimonas sp. isolate AMR_MDS_1826
            NODE_4944_length_5791_cov_2.505927, whole genome shotgun sequence.
ACCESSION   DAOSDR010000202 DAOSDR010000000
VERSION     DAOSDR010000202.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863355
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrimonas sp. (wastewater metagenome)
  ORGANISM  Petrimonas sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Dysgonomonadaceae; Petrimonas.
REFERENCE   1  (bases 1 to 5791)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5791)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:10:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,720
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 35
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5791
                     /organism="Petrimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4944_length_5791_cov_2.505927"
                     /isolate="AMR_MDS_1826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2023866"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(619..2292)
                     /locus_tag="PLI30_08635"
     CDS             complement(619..2292)
                     /locus_tag="PLI30_08635"
                     /inference="COORDINATES: protein motif:HMM:NF013237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent transferase"
                     /protein_id="HOI79656.1"
                     /translation="MKRREIIKTLSAIPVAGGIMSIEPLVSTVNPKKPVNDAGLLSNP
                     EAGALALGPQIYQSIGVEPVINCRGTFTAIGGSIELPEVRKAVDYASQNYVHIDELAM
                     AVGKRLAAITGAEWGMVSSGCAAGMKHVTVACITDGDPEKLVRVPDLTGFTKTEVVVP
                     RYSRNQYDHAIKNAGVKLITVETMEEFKNAINSRTAMIYLFSAPRDYDNGPLSIDNVA
                     AVAREKNIPILVDAAAEILTVPNIHLKRGATIVAYSGGKAIKGPQCAGLLLGNKDLLF
                     SAWQASSPHHGPGRDNKVGKEEHIGMLAAVETWVRRNHPEEEKTWISWLEYISKKVTT
                     ISGVTTKITEPQGVDNRSARLSISWDPEVLNITGEEVAEDLANNKPRIALNGRTNPRE
                     GVASLSISAHMMQPGDEKVVADRIYRILAQKRGKKTVVIEPPAANLTGNWDVSIEYYS
                     GNGEHKLFLEKQEGNDLKGIHKSTFSDLDIAGRIEGKKVRLQSYYREDAESIPFTFDG
                     VMDGESFSGVLYLGEYRSANFIAKRSEYRQRPFNNISVPSYGGKKSDSW"
     gene            complement(2452..3792)
                     /locus_tag="PLI30_08640"
     CDS             complement(2452..3792)
                     /locus_tag="PLI30_08640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI79657.1"
                     /translation="MKNIDRRSFIRNTATASTFAIIAPSLVIPAKDVGEQVEKWQRMM
                     KDPAYEMLNGKPDLDRILGLFPRPQGKPAIDPRVIQANVKKLKTVPEINTGHPYLDLS
                     IKTGLAHIDATFQGDHPKYGVGVYSQVEHDGFPPTIIAAVDALSAWGVNVRASRLFNY
                     WLTNFVKDDGTFDYYGPSISEYGQLLHTATTLEERAGGRGWLNEGLPKLNLIAEYLLR
                     LISSASKENGLISGVPEADTRDQVDIYFHNNGWIVKGLERWINLCEKTRYAPSTSVGI
                     IMKAAQKLKADTLTAIRKTWPDDPCDWWLPVQLGELVRPRYVTETREASYTNYRYWLE
                     LLSSGILPSDLANRLVNARLNGGGQFCGATRFMDWLDDWPLTEYLYGLWTLGRKDDFL
                     LSLYGHIAYSQCEGHLTAYEQMSFPGDPQGSRRADYCLPCQLVAARAGRLINKS"
     gene            4133..5083
                     /locus_tag="PLI30_08645"
     CDS             4133..5083
                     /locus_tag="PLI30_08645"
                     /inference="COORDINATES: protein motif:HMM:NF040125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6528 family protein"
                     /protein_id="HOI79658.1"
                     /translation="MYTELFTKNNPEKINKAVLVNRKLLPVLISLICLFGFMSCNNQN
                     GKKYEDLILVCGDSKVLIAKAAKSNDTIPEIIWSWDAHIANDIPGHFKPERFNTVDDC
                     KAINDGRQIMVSSSSGAVAIINKENKEILFYAEVPNAHSIEILPDNKLVAAASTAKDG
                     NRLMLYERATPPVLLFSDSLYSAHGVVWDTSKNSLFALGYDVLREYKTDNDMLLLQGE
                     WKIPGISGHDLQMAPDGKHLFVTEHTGAWIFSTESKQFSKIDGFPDAENIKSINRNSD
                     GRFVFTVPEESWWTYHVEFSNPSGRLSFPGMHVYKARWFN"
     gene            complement(5437..>5791)
                     /locus_tag="PLI30_08650"
     CDS             complement(5437..>5791)
                     /locus_tag="PLI30_08650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006844172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="arsenical-resistance protein"
                     /protein_id="HOI79659.1"
                     /translation="ALLFTIIVMFSLKGEYIIKIPLDVVRIAIPLVIYFLVMFIISFF
                     MSKRIGANYEQSTTLSFTAASNNFELAIAVAIAVFGINSGEAFAAVIGPLVEVPVMIG
                     LVNVALVFKKNFFQA"
BASE COUNT         1654 a         1265 c         1146 g         1726 t
ORIGIN      
        1 gaaaaaaaag gctgcagata tttagcattc gtgtcgggaa tgataaaatc ggtggaaatt
       61 tcgtccgctt gagggcatcg ccggcaagaa aaaattaact ttaatatcgg tattgactaa
      121 caattgttat ggtctttctc tccttaacct caacgaggta gtggattttt ggataacatc
      181 tttttctaca gtaccaaatg ccctatgggt aaatctttat cgtaactggt accaggtagc
      241 aatgaagata ttgaaaccat aggcctacgt gcaaatattg aggattaccg aataatatga
      301 taccgcttag cggcggttaa ggtattgtag ataattgata aataaataat tatttttccc
      361 cataggggat atggaaataa tttctgacat tgtcctatgt gccccccttg tgtaatcctt
      421 tgattgtggt aaggaaacca gaacaataat tgataaataa ataattattt ttccctgaag
      481 ggaatatgaa gataaaggta tgacctgcca ggtaagttca agtcctccgg atttgtaatc
      541 cggaggactg ctctaagaca agatggctaa ttcgttaaag ccagctatta aggaatatcc
      601 aggccgatcc ggccagtgct accatgaatc gcttttcttt cctccgtagg agggcactga
      661 tatattatta aagggtcttt gcctgtattc cgatcttttt gcaatgaaat tggcagagcg
      721 atattccccg agatacaaaa ctccggaaaa agactctcca tccatcactc cgtcaaaggt
      781 gaaaggtatt gattcggcat cctcgcggta atagctctga agcctgacct ttttcccttc
      841 aattcttccg gcaatgtcga gatctgagaa tgtgcttttg tggatgcctt ttaaatcgtt
      901 accttcctgt ttttcaagga aaagcttatg ttcaccgttg cctgaatagt attctatgga
      961 tacatcccag ttaccggtca ggttagcagc gggaggttca attacaacag ttttctttcc
     1021 acgtttctga gccagtatcc tgtagatccg gtcggcaaca accttttcat ccccgggttg
     1081 catcatatgg gcgctgatgg agagggaggc aacgccttcc cttggattgg tccgtccgtt
     1141 cagtgcgatc cttggcttat tattggccag atcctcagcg acttcctctc cggtgatatt
     1201 aagaacttcc ggatcccatg atatggataa acgggcactt cggttgtcga caccctgtgg
     1261 ttccgtaatc tttgtagtta caccgcttat tgtggttact tttttcgaaa tatactccag
     1321 ccatgatatc caggtctttt cctcttccgg atgatttctt ctgacccatg tttcaacagc
     1381 cgcaagcatc ccgatatgtt cttccttccc aaccttgtta tcccttccgg ggccatggtg
     1441 aggtgaactc gcctgccagg ctgagaacaa caggtcttta ttgccaagaa gaagccctgc
     1501 acactgaggg ccctttattg cctttccacc actatatgca actattgttg cgcctctttt
     1561 caggtgaata ttcgggacag taagaatttc agcagctgca tctaccagta tgggtatgtt
     1621 tttttccctg gcaacagctg ccacattatc tattgataag ggtccgttat cgtaatcccg
     1681 tggcgcagaa aaaagatata tcattgcagt acgtgagttt attgcattct taaactcttc
     1741 cattgtttct acagtgatca attttacgcc ggcatttttt attgcatggt catactgatt
     1801 ccttgagtag cgggggacta ccacttctgt ctttgtgaag cctgtaagat caggaaccct
     1861 taccagcttt tcaggatcgc catccgtaat gcatgctaca gtaacatgct tcattcctgc
     1921 agcgcagcct gaagatacca tcccccattc agctccggta attgcagcca gcctcttgcc
     1981 tactgccatg gctaattcat caatatgaac atagttctgt gatgcataat caaccgcctt
     2041 gcggacttca ggcagttcaa tcgaaccacc gattgcagta aaggttcccc tgcagtttat
     2101 cactggttcc acacctatag actggtatat ctgaggtccc agagccagag ctcccgcttc
     2161 cggattggac agaagtcctg catcattaac aggtttcttc ggatttactg ttgatactag
     2221 cggctcgatg gacattatac ctcctgcaac aggtatagct gacaatgttt taattatttc
     2281 tctgcgtttc atggtaggtt atgttaatag ctttgcaaac aaaaatagga aaaacggaat
     2341 agtatcacca cctttgaatg ttaacaaact ataaattcct taaataagtt gttcctctac
     2401 tgataggaat tcacactatt gctaattata aaccgatata attcataaag cttaagattt
     2461 attgatcaat cgtcctgctc tggctgcaac aagctggcat ggaaggcaat agtcagcccg
     2521 cctgcttccc tggggatcac cgggaaagct catttgctca taagctgtca gatggccttc
     2581 gcactgagaa taagcaatat gaccgtataa actcaacaga aaatcatctt tccttcccag
     2641 tgtccacagg ccatacaaat attcagttaa aggccagtcg tcaagccaat ccataaaacg
     2701 tgttgcaccg cagaattgtc ccccaccatt caaacgagca ttgaccagac ggttggccag
     2761 atcggatggt agtatcccgc ttgataatag ttcaagccaa taacgataat ttgtataact
     2821 ggcttcccgg gtttctgtaa catagcgtgg ccgtacaagt tcacccaatt gaacaggaag
     2881 ccaccaatca catggatcgt caggccaggt tttcctgatc gcagtcagtg tatctgcctt
     2941 cagtttctgg gcagccttca tgatgatccc gacagaagtg gatggagcat atcttgtctt
     3001 ttcacataaa ttaatccacc gctctagtcc ttttacgatc cagccattat tatgaaaata
     3061 aatatcgacc tggtcacggg tatcagcctc gggaacaccc gaaataagcc cattttcttt
     3121 agatgccgat gatattaatc gtaacaggta ttctgcaatc aggttgagct ttggcaatcc
     3181 ttcattcagc catcctctgc caccagctct ttcttccagg gttgttgctg tatgcaacaa
     3241 ctgcccgtac tcgctgatgg atggtccata ataatcgaat gtgccatcat ctttaacaaa
     3301 attagtcaac cagtagttaa aaagccggga agcacgaaca tttacacccc aggcagataa
     3361 tgcgtcaact gcagcaataa tagttggtgg aaatccatca tgttcaacct ggctataaac
     3421 tcccactcca tatttcggat gatcaccctg aaaagtggca tcgatatgag ccaggcccgt
     3481 ttttatagat aaatcaagat acggatgccc ggtattgatc tcagggactg tcttcagctt
     3541 tttaacgttt gcctgtatca ccctgggatc aattgccggt ttcccttgcg gtctgggaaa
     3601 caatcccaga atcctgtcaa ggtcaggttt tccattaagc atttcatatg caggatcttt
     3661 catcatcctc tgccattttt ctacctgttc gccaacatct tttgccggaa tgacaagtga
     3721 tggagcaatt attgcaaatg ttgaagcagt tgccgtattt cttataaaac ttctcctatc
     3781 aatattcttc attgaattaa gatttgaata atcaagactt ataatcttta tgatcaacta
     3841 tttaaagcga tgtatcatag tccggttagt attatcctta tgataaggaa ttcacattaa
     3901 ttccaatata ttgaattagt ttcttctccg tttatccgga cattaagtta agctaaaatg
     3961 tttactttaa ttgctactga tagatggaag attttgtttt cagaatctct gcatgagtta
     4021 acctggcatg agtttgtaca taaaagtgtg tcattttcat cttcacttat gcatcattga
     4081 aaaaagtttt ataaatagca gccggagtta atcattataa taagctgcga aaatgtatac
     4141 agaacttttt acaaaaaata atccggaaaa gattaataaa gctgtacttg tcaatagaaa
     4201 attgctgcct gtcctgattt cattaatttg tttattcggt tttatgtcgt gtaacaacca
     4261 aaatgggaaa aaatacgaag atctgatctt ggtttgcggc gacagcaaag ttcttattgc
     4321 aaaagctgca aaatcaaacg ataccattcc ggaaattatc tggagctggg atgcacatat
     4381 tgcaaacgat attcccggtc atttcaaacc agaaaggttt aatactgttg acgattgcaa
     4441 agcaattaac gacggcagac agatcatggt ttcatcttca tcgggcgcag tggcaataat
     4501 caacaaagaa aataaggaaa tccttttcta cgctgaagtt cccaacgcac attccattga
     4561 aattttgcct gacaacaaac ttgtagcagc agcttccacc gcaaaagacg gaaacaggct
     4621 catgttgtat gaaagagcca ctccgccggt tttgttattt tccgacagtc tgtattcggc
     4681 acacggagtg gtttgggata cttcaaaaaa ctcactattt gctttagggt atgatgtttt
     4741 gcgcgaatac aaaacggaca acgatatgct tttgcttcag ggagagtgga aaatccccgg
     4801 aatcagcggg cacgatctgc aaatggctcc tgacgggaag catctttttg taacggaaca
     4861 caccggcgca tggattttta gtacagagag taagcagttc agcaaaattg acggcttccc
     4921 cgatgccgaa aacataaaaa gcatcaaccg gaatagtgat ggacgctttg tttttaccgt
     4981 acccgaagaa agctggtgga cttaccatgt tgaattttcc aatccttccg gaagactttc
     5041 atttcccggc atgcatgttt ataaagctag gtggtttaac taaactcaag gcaaccattt
     5101 tttaattttg tgaatccatg atttctttgt agcgatagag ggaattgcct ttgcagattt
     5161 cgttgggtga taatttaaaa ggaaaagcct ggcaaaactg cgtgtttagg tgcaaaagcc
     5221 gtttaccact atacgccaga gttaattgac cacctcccac cggttttaaa tatctgttgt
     5281 catgtgactt atattgatga actccagact ggccagaaat ggtcaaggct ttagcaattt
     5341 tttaaacttc ccttcaggtg atagaagatt gccgaattat taaaaaaaga ggtctattat
     5401 gacctctcaa aattcataat tctgacattt gaaaatttaa gcctgaaaaa aatttttctt
     5461 aaataccagg gccacattta ccagacctat catcacagga acttcgacaa gcggaccaat
     5521 caccgctgca aatgcttccc ctgaatttat accaaacact gctattgcaa ctgcaattgc
     5581 caattcaaaa ttattactgg cagcagtaaa agaaagcgtt gtagattgtt catagtttgc
     5641 cccaattctt ttgctcataa aaaatgatat tatgaacatc accagaaagt aaataaccaa
     5701 gggtatggcg atacgaacaa cgtccaaagg gatttttatg atgtattccc ctttcaatga
     5761 aaacattacg atgattgtaa ataacagtgc t
//
LOCUS       DAOSDR010000203         5787 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrimonas sp. isolate AMR_MDS_1826
            NODE_4949_length_5787_cov_3.483426, whole genome shotgun sequence.
ACCESSION   DAOSDR010000203 DAOSDR010000000
VERSION     DAOSDR010000203.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863355
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrimonas sp. (wastewater metagenome)
  ORGANISM  Petrimonas sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Dysgonomonadaceae; Petrimonas.
REFERENCE   1  (bases 1 to 5787)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5787)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:10:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,720
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 35
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5787
                     /organism="Petrimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4949_length_5787_cov_3.483426"
                     /isolate="AMR_MDS_1826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2023866"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..734
                     /locus_tag="PLI30_08655"
     CDS             <1..734
                     /locus_tag="PLI30_08655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006800449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HOI79660.1"
                     /translation="SIDFEGLKEIIAENDCKMLILCNPHNPVGRVWNEDELIQLAEIC
                     CDNNILVVSDEIHSDLTLPGFRHRPFATVSEKAKENSITLMAPSKTFNIAGIVSSFAV
                     IHNPEIRKQYLNYLAPRELDQATIYALAATEAAYNYGEEWLDQALAYIQKNIDFVENY
                     LKEYIPQINAVKSEATFLVWLDCRALNLTQQELVDLFVNKAKLALNDGTVFGKEGEGF
                     MRLNVGTPLSNIEKALDNLRKALNS"
     gene            795..1382
                     /locus_tag="PLI30_08660"
     CDS             795..1382
                     /locus_tag="PLI30_08660"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018108303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HOI79661.1"
                     /translation="MKISDSKYVTLTYDLNVGEGDERELMEQATPEKPLEFIFGTNSM
                     LKAFEDNIYGLSEGDPFKFTLSPEEAYGDFDESRIIDLPKNIFEVDGKIDNEMLFEGN
                     TLPMMDTDGNRLMGSVVSITGDTVTMDFNHPLAGETMHFEGTVQGVRDASPEEIAALF
                     SGSGCGCGSGGCGGCDEETESGGCGSGGCGSGCSC"
     gene            1469..3586
                     /locus_tag="PLI30_08665"
     CDS             1469..3586
                     /locus_tag="PLI30_08665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019539707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HOI79662.1"
                     /translation="MILALDLALPVSNPTVQFLLLFSIVLIAPIVFNRIKVPSLIGLI
                     IAGAVIGPFGLFIMSRDSNMIMLGNAGLLYIMFLAGIEIDVAEFKKNSSRSLVFGLLT
                     FSIPMTIGVFAGYYLLSLSLISSVLLASMFASHTLIAYPIISKLGAGKNRAVTVAIGG
                     TMITDTLALLVLAVIISMNEGQVTTAFWAQLTLSIIVFTLVVAFLFPLVGRWFFKKFQ
                     DSASQFIFVMVMIFLGGFLAELAGIEAIIGAFLAGLAMNKLIPHISPLMNRIDFVGNA
                     IFIPIFLISVGMLIDYRAFVTGFETIKVAIVMTVVAISAKFLAAWATQKIYGYSNAER
                     KLIFGLSNAQAAATLAAVMVGHRVGLLDDNILNGTIVMILVTCTIASFRAQKGAIDLS
                     FAEGNLEDENTLTEERILIPIANKDTLKDLVELSLVIKSKTNTQDLYALNIIKSTGTT
                     PQAEKESLKLLEEAVRLGAGAETVIQPLRRYDMNVLNGILGIVKKYAITDVVMGLHVK
                     QEISESFLGDLIEGILEKTRSTTFVYKAIQPLSTIKKRLVIIPPNADKEIGFPFWLSK
                     IWNLGRNTGAKLSFYATNDILEILKQINKTHPIDVDFKEFSDWNDFLVLSKEVGKDVG
                     LIIVMSRLRKASYHETMKRVPDYINKYFKNTNFMLLYPVQSATTDNYHNDMANASLLA
                     SIRNLGGFGEALLSTVKKSDPSE"
     gene            3661..4722
                     /gene="aroC"
                     /locus_tag="PLI30_08670"
     CDS             3661..4722
                     /gene="aroC"
                     /locus_tag="PLI30_08670"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006799517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HOI79663.1"
                     /translation="MNTFGNIFRLTSFGESHGEAVGGVIDGCPPGIELDLEFIQNELD
                     RRRPGQSRITTPRVESDKVVFLSGIFEGKTTGTSIGFIIKNENQHSADYNNLKDIYRP
                     SHADYTYFQKYGIRDHRGGGRSSARETIARVVAGAVAKLYLKQAGVSVTAYTSQVGEI
                     KLDNNYKQYDLTTIEDTPVRCPDTEKANEMINLIKEVQYKGDTIGGVVTCVIQGTPVG
                     LGEPVFGKLHAALGSAMLSINAVKGFEYGHGFDVSRRGSEMNDSFFNDNGKISTRTNY
                     SGGIQAGISNGQDIYFRVAFKPVSTILMEQQTVDVHGNDTTIKARGRHDACVLPRAVP
                     IVEAMAAMVILDYYLLAKM"
     gene            4938..5510
                     /locus_tag="PLI30_08675"
     CDS             4938..5510
                     /locus_tag="PLI30_08675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019539005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOI79664.1"
                     /translation="MWQKFIKERDDLSFSLVYNTYVEVLYAYGIHLGFRDELCKDAIQ
                     DVFYNIYVKKSELKKVKNPTAYLFKSYKYRLIDLARKEAKIETIDSCSDSFTLDITIL
                     DNIIDAEESILLKNKVKKLLECLSGQQREAVYMKYMLGLGYDEISEILQINPDSVRKL
                     MYRSMEKIRQNIDNDHPEWLPLFIVLFLCF"
BASE COUNT         1647 a         1325 c         1325 g         1490 t
ORIGIN      
        1 actctatcga ttttgaagga ttaaaggaaa ttatcgccga aaacgattgc aagatgctta
       61 tcctctgcaa tcctcacaat cctgtaggaa gggtatggaa tgaagatgaa ctgatacaac
      121 tggcggaaat ctgttgtgac aacaacatcc tggttgtttc cgacgaaatt cattccgacc
      181 tgaccttgcc cgggttcagg caccgaccct ttgccacggt gtcggaaaag gcaaaagaga
      241 acagtatcac gctgatggcc ccaagcaaaa ccttcaacat tgccgggatt gtcagttctt
      301 ttgccgttat ccacaacccg gagataagga agcagtactt gaattacctg gctccccggg
      361 aactcgacca ggcaactatt tacgcactgg ctgctaccga agccgcatac aattacggtg
      421 aggaatggct ggatcaggca ctggcataca ttcagaaaaa tatcgatttc gttgaaaatt
      481 acctaaagga atacattcca caaatcaacg cagtgaagtc tgaagctacc tttctcgtct
      541 ggctggattg cagggcgttg aaccttacgc agcaggaact ggttgacctg tttgtcaaca
      601 aagcaaaact ggcgctaaac gacggcactg ttttcgggaa ggaaggagaa ggatttatgc
      661 ggttgaacgt gggtacaccc ctttcgaaca tcgagaaagc actcgataat ctgagaaaag
      721 cgttaaacag ctaaaactat ttcccccgca cattgttaaa atttcacaca cttaatcata
      781 taaaaacaaa ttcaatgaaa atttctgaca gcaaatatgt aaccctgaca tacgatctaa
      841 acgtaggcga aggggacgaa cgtgaactca tggaacaggc cactcccgaa aagccgctcg
      901 aatttatttt cggcacaaac tcgatgctga aagctttcga agacaatatc tacgggcttt
      961 cggaaggcga tccatttaag tttacactga gtcctgaaga ggcttacggt gattttgacg
     1021 aatcaaggat cattgacctc cccaaaaaca ttttcgaggt ggacggaaag atagacaatg
     1081 aaatgctttt cgaaggaaat acattgccca tgatggatac cgatggaaac cgactgatgg
     1141 gatcggttgt ctccattact ggcgacacgg taaccatgga tttcaaccat cccctggcag
     1201 gcgaaaccat gcattttgaa gggactgtgc aaggtgtacg tgacgcctct cccgaagaga
     1261 tagcggccct gttttccgga agtggatgcg gttgcggaag tggaggttgt ggcggttgcg
     1321 atgaggaaac cgagtccgga ggctgtggaa gcggcggttg cggaagcggt tgtagctgtt
     1381 aaccgtttcg tcccaaaaag taaaaaatca cagacatgga taatgcctgc gattttttta
     1441 tatacacgtt tattgtaagc ccaagcttat gattctggca ttagatctgg cattacccgt
     1501 ttcgaacccg acggtccagt ttctgctcct gttctcgatt gtcctgattg cacccattgt
     1561 tttcaatcga ataaaagtgc cgtcacttat cggactaatc attgccggag ctgtaattgg
     1621 ccctttcgga ctgtttatca tgtcgcgtga cagcaacatg atcatgctcg gcaatgccgg
     1681 cttgttatac atcatgttcc tggcaggcat tgaaatcgac gttgccgagt ttaaaaaaaa
     1741 cagctccaga agcttggttt ttggcctgct caccttttca attcctatga caataggtgt
     1801 ttttgccgga tattacctgc tcagcttaag cttgatctct tcggtgctgc tggccagtat
     1861 gtttgcgtca cacaccctta ttgcctaccc cataatcagt aagttaggag ccgggaagaa
     1921 cagagcggta accgttgcta tcggcgggac tatgattacc gacacgctgg cattgttggt
     1981 gctggcagtt atcatcagca tgaacgaagg ccaggtcaca actgcgttct gggcacaact
     2041 gacactctcc atcatcgtct tcacactggt ggttgctttt ctttttcccc ttgtaggcag
     2101 gtggtttttc aaaaaattcc aggacagtgc gtcgcagttc attttcgtga tggtgatgat
     2161 ctttctggga ggtttcctgg cagaactggc ggggattgaa gccatcatcg gcgcctttct
     2221 ggccggattg gctatgaaca agcttattcc acacatttca ccgctgatga accggatcga
     2281 tttcgtagga aatgccattt tcattcccat atttctgatc agtgtgggga tgcttatcga
     2341 ttaccgggcg ttcgttaccg gatttgaaac tatcaaggta gccatcgtga tgacggttgt
     2401 ggccatttca gcaaagtttc tggcggcttg ggccacacag aaaatttacg gttattccaa
     2461 cgccgaacgg aagcttattt tcggcttgag caacgctcag gccgcagcca cgttggctgc
     2521 tgtcatggta ggacatcgag tgggcttact cgacgacaac atcctgaacg gcaccatcgt
     2581 gatgattctc gttacgtgca ccatcgcttc gtttcgtgca cagaaaggtg ctatcgacct
     2641 ctcttttgcc gaaggtaacc tggaagacga gaacacctta accgaggaac ggatcctcat
     2701 ccccatcgcc aacaaggata cgctgaaaga tttggtcgaa ctgagcctgg ttattaaatc
     2761 caaaaccaac acgcaggatt tatatgcgct caacatcatc aagagcaccg ggacaacacc
     2821 tcaagcggag aaagagtcgc taaagctact ggaagaagcc gtcaggctgg gtgcaggtgc
     2881 agaaaccgtt attcagcccc tccgccggta cgacatgaat gtgttgaacg gaattttggg
     2941 tattgtaaaa aaatacgcca tcaccgatgt ggttatgggt ttgcatgtaa aacaggaaat
     3001 atcggaatcg tttctgggcg accttatcga gggtatcctg gaaaaaacac gttcaaccac
     3061 ctttgtttac aaagccattc agccgctatc gacaatcaaa aagaggttgg taatcattcc
     3121 tcccaacgcc gataaggaaa tcgggttccc gttctggctt tcgaaaatct ggaacttggg
     3181 cagaaatacg ggagcaaaac tcagttttta cgccaccaat gatatacttg aaattttaaa
     3241 gcaaatcaac aaaacacatc ccattgatgt tgattttaaa gaattcagcg attggaacga
     3301 ttttttggtc ttatcgaagg aagttggtaa agatgtggga ttgattatcg tgatgagccg
     3361 tttacgcaaa gcttcgtacc acgaaaccat gaaacgtgtt cccgattaca tcaacaagta
     3421 tttcaagaac accaacttca tgctgctcta tccggttcag tcggccacta cggataatta
     3481 tcacaacgac atggccaacg cctcactgct tgcctccatc aggaatctcg gcggatttgg
     3541 cgaagccctc ctttcgacgg taaagaaaag cgacccgtct gagtaacgga tgatctcaac
     3601 aaattgtgct aattcatctt tttcacgtta attaatcgta tttttgcaaa aaagaactca
     3661 atgaatactt tcggaaatat tttcaggctt acatcgtttg gagagtccca cggtgaagcc
     3721 gtggggggag ttattgacgg ttgcccgccg gggatcgagc tggatttgga attcatccaa
     3781 aacgaactcg acaggcgacg cccaggacaa tcgcgcatta ccacaccacg cgtcgaatcg
     3841 gacaaggtgg tttttctgtc cggcatcttc gaaggcaaaa caaccggaac ctccatcggt
     3901 tttatcatta agaatgaaaa tcaacactcg gccgattata acaacctgaa agatatttat
     3961 cgcccttcac acgccgatta tacctatttt caaaaatacg gcatccgcga tcaccgcggc
     4021 ggtggccggt catcagcacg agaaaccata gcccgagtag tggcgggagc tgtggccaaa
     4081 ttgtacctga agcaggccgg tgtatcggtt accgcctaca cttcacaggt aggtgaaatt
     4141 aaactggaca acaactacaa acaatacgac cttaccacta tcgaagacac gcccgttcga
     4201 tgccccgata ccgaaaaggc aaatgagatg atcaacctta tcaaggaagt ccagtacaaa
     4261 ggggatacca tcgggggggt ggttacctgc gttatacagg ggacacccgt aggccttggc
     4321 gagccggtct ttgggaagct gcacgcggca ctgggctcgg ccatgctcag cattaacgcc
     4381 gtgaaaggat ttgagtacgg acacggcttc gatgtgagtc gccggggttc ggaaatgaac
     4441 gattcatttt tcaacgacaa cgggaaaata agtacccgca ccaactattc cggcggtatc
     4501 caggcaggta tttccaacgg acaggatatt tatttccggg tggccttcaa acctgtttcc
     4561 accattttaa tggaacagca aaccgtggat gtccacggaa acgacacgac cattaaggcc
     4621 cgcgggcgac acgacgcatg cgttcttccc cgtgctgtgc ccatcgttga ggctatggcc
     4681 gccatggtta tcctggacta ctacctactg gctaaaatgt agttttcctg tcgcccattt
     4741 gcctttacaa agattttttg catctttgca aattattgta ttttaacaac agggtctgtc
     4801 ccgaaaataa aaaaatgatt ttcacaatcc ataatataca gataatcaga ttatgcttgt
     4861 tggcatttga ggtaaaaatc actttttgag gagactaaat acaagatacc ctactgtaag
     4921 tgatgaacac ggatttgatg tggcaaaaat ttatcaaaga aagagacgat ctttctttct
     4981 ctcttgtata caatacctat gtggaagtac tatatgccta cggaattcac ttgggctttc
     5041 gtgacgaact atgtaaagat gccatccagg atgtttttta caacatatat gtaaagaaaa
     5101 gcgaattaaa aaaagttaaa aaccccacgg catatctttt taaatcttac aaataccgcc
     5161 ttatcgacct tgcccgtaaa gaagcaaaaa ttgaaaccat agactcgtgc agcgattctt
     5221 ttacactaga catcaccatt ttagacaata taatcgatgc ggaagaatcg atcctactga
     5281 agaacaaggt taaaaaattg ctggaatgcc tgtcggggca gcagcgtgaa gccgtgtata
     5341 tgaaatacat gcttggcctg ggttacgacg agataagcga aatattgcag attaatcccg
     5401 attccgtaag aaaattaatg taccgctcca tggaaaaaat aagacaaaac atcgataatg
     5461 accaccccga atggctaccc ttgttcatag tcctgttcct ctgcttttag gtatccctac
     5521 gggtttctcc ttccaagacg ttaatttttt atttttttta ttttttttgt ccacaaatga
     5581 aaaacataaa cgtcctatat ataaaataca atatatgtga aaaacaaatg gtatttcctt
     5641 ttcaatgaag attttataca atggagactt ttccggacgg aggagcaaaa cggacaatgg
     5701 gagaaattca ggttagagaa tccgcatctt gaagagcact tggacgaagc cataagacgg
     5761 ttcaatgccc tggagatgaa tcgcgtc
//
LOCUS       DAOSDR010000204         5774 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrimonas sp. isolate AMR_MDS_1826
            NODE_4966_length_5774_cov_3.898409, whole genome shotgun sequence.
ACCESSION   DAOSDR010000204 DAOSDR010000000
VERSION     DAOSDR010000204.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863355
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrimonas sp. (wastewater metagenome)
  ORGANISM  Petrimonas sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Dysgonomonadaceae; Petrimonas.
REFERENCE   1  (bases 1 to 5774)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5774)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:10:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,720
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 35
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5774
                     /organism="Petrimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4966_length_5774_cov_3.898409"
                     /isolate="AMR_MDS_1826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2023866"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..703
                     /locus_tag="PLI30_08680"
     CDS             <1..703
                     /locus_tag="PLI30_08680"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019539395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOI79665.1"
                     /translation="TAYDQGKIEFVACSQWLGRLAEKSPLTTGRRVVSIPNPIDTQRY
                     FPKNKEEAKQKLGLPADKRIILFAAVKASDKRKGMDYLIEAGKRLNNKEVLFLIAGTN
                     GNEIAQQLPALSVNTGFVTPDRMPDLYNAADVFVTPSLQENLPNTLMEAMSCGTPCVG
                     FNTGGIPEMIDHRKNGYVAEYKDADDLTQGLQWILFEADFRQLSENARKKVLSEYAEE
                     IIVNRYLTIYAEQSQ"
     gene            684..1409
                     /locus_tag="PLI30_08685"
     CDS             684..1409
                     /locus_tag="PLI30_08685"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HOI79666.1"
                     /translation="MQNNPNNITDQSHWEAYWSDYQFDKIPRSVVFGKFIPKLSGAKS
                     FIEIGGFPGIFAAYFYKQGIPDVTVLDFYMNTAIVRKFEAVNNLPEHTIRCIDGDFFE
                     ISSEKKYDVVFSSGFIEHFQDTRDVIARHVDLLSENGHLLLLVPNFLGLNGRIQQWLD
                     RENLEAHNLKSMEIPRLKGIMQEFDLKEVEIEYLGKPMLWLEPKPKNKRIRKWVKMLS
                     YAVKLFPVKGRFLSPFIVVYARK"
     gene            1406..2143
                     /locus_tag="PLI30_08690"
     CDS             1406..2143
                     /locus_tag="PLI30_08690"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019539397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOI79667.1"
                     /translation="MKSLSVITVTYNAGKTLERTLKSVREQTYPYVEHIIVDGKSKDN
                     TLALIQQYENPKLKWKSEPDNGLYDAMNKAVTMATGDYLCFLNAGDMFFLPETVEKMM
                     NSFEPASAPDILYGETAIVGDNGEFLFMRRLSAPETLSWKSFKQGMLVCHQAFMVKKS
                     IFEPYDLNYRFSADVDWCIRMMRKSRYIRNTHLTLINYLNEGITTANRKASLKERYRI
                     MVKHYGQVSTFLHHVWFAVRAVIKPEK"
     gene            2185..2721
                     /locus_tag="PLI30_08695"
     CDS             2185..2721
                     /locus_tag="PLI30_08695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008146775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HOI79668.1"
                     /translation="MYRRKLNVEELNRISVKEFREAPKIPLVVVLDNVRSRHNIGSVF
                     RTCDAFRVEEIILCGIAAPPPDAEIHKTALGAEDSVKWRYFAEPISAIKELKTNGYTV
                     FSIEQTRNSLSLETLTLDRRGRYAVILGHEVHGVQQEVVDASDNCIEIPQYGTKHSLN
                     VSVTAGIVIWDFFKQLSE"
     gene            2718..3083
                     /locus_tag="PLI30_08700"
     CDS             2718..3083
                     /locus_tag="PLI30_08700"
                     /inference="COORDINATES: protein motif:HMM:NF016056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DCC1-like thiol-disulfide oxidoreductase family
                     protein"
                     /protein_id="HOI79669.1"
                     /translation="MTVYYDNWCKNCTRFMNLIRQLDWFNLISCRELRNEEHTKIAPG
                     LNLTLAEEQLASVHKGIWKYGYDSLYRTILRLPLFWLLVPAFWVLKITRLGSCLYKQL
                     AVNRHIVPLHCRQKDCERI"
     gene            complement(3080..4348)
                     /locus_tag="PLI30_08705"
     CDS             complement(3080..4348)
                     /locus_tag="PLI30_08705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019538785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase U32 family protein"
                     /protein_id="HOI79670.1"
                     /translation="MKKIHDYEIMSPAGSYESLTAAIQGGADSIYFGIEGLNMRAKSS
                     NNFTVEDLHKIAEICRENKIKSYLTVNTIIYDNDMELMRKVVNAAREANLSAIIAADV
                     AVLMYARSIGVEVHLSTQLNITNTESLRFYAQFADVVVLARELNLDQVASIHKDIVEQ
                     QIKGPSGDLIRIEMFAHGALCMAVSGKCYLSLHEKNLSANRGACNQICRRGYIVKDKD
                     SEIELEIDNEYIMSPKDLKTIHFMNKMMDAGVRVFKIEGRARGPEYVRTVTSCYKEAA
                     QAYCNDTFSQEKIDTWNEKLATVFNRGFWDGYYLGQRLGEWTHRYGSGATKRKVYVGK
                     AIKHFGNIGVTEFLVEAQPVKEGDELLITGPTTGAVFVTAEDIRVDLRQVSEAVKGDH
                     FSIKTPEKIRPNDQLYKMVKAERRGTAHER"
     gene            4455..5354
                     /locus_tag="PLI30_08710"
     CDS             4455..5354
                     /locus_tag="PLI30_08710"
                     /inference="COORDINATES: protein motif:HMM:NF015907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004867 - serine-type endopeptidase
                     inhibitor activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ecotin family protein"
                     /protein_id="HOI79671.1"
                     /translation="MKKFYSLLFLLTLLVLLTNCSQGKSTSSDNPYRLDSIEMTGILK
                     KYPHPQGYQKYVISVPGKPDESQFIVEIIPGKTMQMDECNDYSLQGEFVEDSFADEDV
                     LRYIYRSEGEVVQTLLGCATDSLHTEFARGMPVLVDYNSLRPTVVYLPKDIELKHTVW
                     KVAEMIQVEHDPENKLFEKRPFDLTKDKYDGYILQLPAGSENSKIELVVGITKKVDCN
                     RHRLIDVTFGDAGDMNFSPHMFMIFDSDGEIASTRMGCPDRELTGKFIYGETRRVPYT
                     TDPMIIFIPKGFELRYRIWESVV"
     gene            5574..>5774
                     /gene="rpsF"
                     /locus_tag="PLI30_08715"
     CDS             5574..>5774
                     /gene="rpsF"
                     /locus_tag="PLI30_08715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005865478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HOI79672.1"
                     /translation="MNNYETVFIVTPVLSDVQMKEAVEKFKNLLTDQGAEIVNEEDWG
                     LRKLAYPIDKKTTGFYTFLEFNA"
BASE COUNT         1570 a         1231 c         1355 g         1618 t
ORIGIN      
        1 gacggcatac gatcagggaa aaatagaatt tgtggcctgc agccagtggc ttggaaggct
       61 agcagaaaaa agtccactta caacaggacg ccgggtagtt tctattccaa accccatcga
      121 tacgcaaagg tactttccca aaaataagga ggaagcaaaa caaaaactgg gcttacctgc
      181 ggacaaaagg attattttgt ttgccgcggt taaagcttcc gacaaacgaa aagggatgga
      241 ctacttgata gaagccggca aacgacttaa caacaaagag gtgctgttcc tgattgccgg
      301 gacaaacggg aacgaaattg cacagcaact gccggccttg tccgtgaata ccggatttgt
      361 taccccggat agaatgccgg atctttataa tgctgccgat gtttttgtga caccttcact
      421 gcaggagaac ctaccgaata cccttatgga ggctatgtca tgcggtacac cttgtgttgg
      481 attcaatacc gggggaatcc ccgaaatgat tgaccaccga aaaaacggat atgtggccga
      541 atataaggac gctgatgact taacccaagg tttgcagtgg atcctttttg aagctgactt
      601 ccggcagctt tcggaaaatg cccgaaaaaa agtattgtcg gaatacgcag aggaaataat
      661 tgtcaaccgc tatttaacga tttatgcaga acaatcccaa taatattacc gatcagtcgc
      721 actgggaggc atactggtcg gactatcaat ttgacaagat tcctcgcagc gtggttttcg
      781 gaaaatttat accgaaactc agcggagcca aaagttttat cgaaatcggc ggttttcccg
      841 gtatttttgc ggcgtatttt tacaagcagg gtattccgga tgttaccgta ctcgactttt
      901 acatgaatac tgctatcgtc cgtaagtttg aggcggtcaa taatcttccg gagcatacca
      961 tccggtgtat tgatggcgat tttttcgaaa tttcgtccga aaaaaaatac gatgtagtct
     1021 tctcatcggg atttatcgaa cactttcagg acacgcgcga cgtcattgca cggcacgtgg
     1081 acttgctctc ggaaaacgga catttactcc tgctggtccc taattttctc gggttgaacg
     1141 gaagaataca acaatggttg gatagggaaa atctggaagc acataacctg aaatcgatgg
     1201 agatcccacg tttgaaaggc attatgcagg aattcgattt aaaggaggtt gaaattgaat
     1261 acctggggaa gcctatgctt tggctggagc cgaaacccaa aaataagcgt atccgaaaat
     1321 gggtgaaaat gttgagttat gccgttaaac tttttccggt gaagggacgg ttcctttcgc
     1381 cgtttattgt tgtttacgcc cgcaaatgaa atcgctttcc gtaataaccg ttacctacaa
     1441 cgcaggaaaa acgttggaac ggacgctgaa aagtgttcgc gaacaaacgt atccgtatgt
     1501 ggaacatatt attgtggacg gaaaatcgaa agacaatacc cttgcgttga tccagcagta
     1561 tgagaatccc aaactgaaat ggaagagtga gccggacaac gggttgtacg acgcaatgaa
     1621 taaagccgta acgatggcta cgggtgacta tctttgcttt ttaaatgcag gtgatatgtt
     1681 ttttctgccg gagacagtag agaaaatgat gaattctttc gaacccgctt cggcccctga
     1741 catcttgtat ggtgaaacgg ctattgtcgg tgataacggg gaatttttgt ttatgcgtcg
     1801 actaagtgca cccgaaaccc tttcttggaa aagttttaaa caaggaatgc tggtgtgcca
     1861 tcaggcgttt atggtcaaga aatcgatttt tgagccttac gacctgaatt atcgtttttc
     1921 tgccgatgtg gactggtgca tccgcatgat gagaaaatcc cgctacatcc gtaatacaca
     1981 cctgacgctt attaattacc tgaacgaggg aataactacg gccaaccgga aggcttcgct
     2041 caaggaacgg taccggatca tggtgaagca ttacggacag gtgtcaactt tccttcacca
     2101 tgtttggttt gcagtgagag ccgtaataaa acctgaaaaa taacctcccc tttctcttaa
     2161 gtgatgggga gagaaacaga gatgatgtac agaagaaaac tcaacgttga ggagctcaac
     2221 agaataagtg ttaaagaatt tcgagaagca cccaaaattc ccttggtggt ggtgttggac
     2281 aatgtgcgca gccgacacaa catcggttcg gtttttcgca catgcgatgc tttccgggtg
     2341 gaggagatta tcctttgtgg cattgctgcc ccaccgcccg atgccgaaat tcataaaacg
     2401 gcactgggtg ctgaagattc ggtgaaatgg aggtatttcg cggaacctat ttcggcgatt
     2461 aaagaattga aaacgaacgg gtataccgtt ttttccatag agcagacccg gaacagcctt
     2521 tcgttggaaa ccctgacgtt ggaccggcgg ggaagatatg ccgtcatcct tggacatgaa
     2581 gtacacggtg tgcaacagga agttgttgat gcttcggaca actgcatcga aattccgcag
     2641 tacggtacca agcactcact gaatgtttcg gttacggcgg gaatagtgat ttgggatttt
     2701 ttcaaacaac tgtctgaatg actgtttact acgataattg gtgcaaaaac tgcacccggt
     2761 tcatgaacct gatccgtcag ttagattggt ttaatcttat ctcatgcaga gagttacgta
     2821 atgaagagca tacgaaaatt gcacccgggt tgaatctgac tttggcggaa gagcaattgg
     2881 catctgttca caaagggata tggaagtacg gatacgattc gctgtatcgg actattctcc
     2941 ggcttccgct gttctggttg cttgttccgg ctttctgggt attgaaaatc acccggttgg
     3001 gctcttgttt gtataaacaa ttggctgtaa acaggcatat agttccgttg cactgccggc
     3061 aaaaagactg cgagaggatt taacgttcgt gagctgtccc gcggcgttca gcctttacca
     3121 ttttgtaaag ctggtcgttt gggcggattt tctcgggcgt ttttatcgag aagtgatcgc
     3181 ctttaactgc ttcggaaact tgcctcaaat cgactcggat atcttctgcc gtcacaaaaa
     3241 ccgcgccggt ggtcggcccg gtgatcaata actcatcgcc ttccttaacc ggttgggctt
     3301 ctaccaaaaa ttccgtaacg ccgatgttcc cgaaatgctt aatggctttg cccacataaa
     3361 cttttctttt tgttgcccct gaaccgtacc ggtgcgtcca ttcacccaac cgttggccga
     3421 ggtaataccc gtcccagaat ccgcgattaa agacggtggc cagcttttcg ttccatgtat
     3481 cgattttttc ctgcgaaaac gtatcgttgc agtaggcctg ggccgcttct ttgtagcacg
     3541 aggttactgt ccggacgtac tccgggccgc gggcacggcc ttcaatctta aagacccgga
     3601 cacccgcatc catcatctta ttcatgaaat ggatggtctt gaggtctttg ggagacatga
     3661 tgtactcgtt gtcaatttcc agctcaattt cgctgtcctt gtcctttacg atatatccac
     3721 ggcggcaaat ctggttgcat gctccccggt ttgccgacag atttttttcg tgtaagctta
     3781 gatagcattt gcctgaaaca gccatacata aagcaccgtg cgcgaacatt tctatgcgta
     3841 tcagatcacc cgacgggccc tttatctgct gttcgacgat gtctttgtga atggaagcta
     3901 cctgatcgag gttgagttcg cgagccaaca ccaccacatc ggcaaactgg gcgtaaaacc
     3961 gtaacgattc ggtgttggtg atattcagct gggtagacaa gtgtacttcc actccaatgc
     4021 ttcgggcata catcaacacc gccacgtctg ctgcgatgat tgctgataaa ttggcttccc
     4081 tggctgcatt caccactttt cgcatcagct ccatgtcgtt gtcataaata atggtattga
     4141 cggtcaggta actttttatt ttattttccc tgcagatctc cgcaattttg tgcaggtctt
     4201 ccacggtgaa attgttggac gacttggcac gcatgttcag cccctcgatg ccaaaataga
     4261 tagaatcagc acctccctga atggcagctg tcagtgattc gtaggaacct gccggtgaca
     4321 tgatttcgta atcgtgaatt tttttcatct ctgtgatttt ttgagataca aaaatacgac
     4381 tttttacgca ataggcatag aactatttga ataaggaaac gtttaacaga taacaaaacc
     4441 ctaaaatagc tattatgaaa aagttttatt cccttctttt cttacttaca cttttggttt
     4501 tacttacaaa ctgttcgcaa gggaaatcga catcatctga caatccttat cggttggatt
     4561 caatagagat gactggaatc ctgaaaaaat atcctcatcc acaaggttat caaaaatatg
     4621 tcatttccgt gcccgggaag cctgatgaat ctcaatttat cgttgagata atcccgggta
     4681 agacgatgca aatggatgaa tgcaacgatt atagcttaca gggtgagttt gtagaagatt
     4741 cttttgccga tgaagatgtg ttacgctaca tttaccggtc ggaaggggaa gtggtgcaga
     4801 cgttgctggg atgtgcaaca gacagcctgc acaccgagtt tgctcgtggg atgccggttt
     4861 tggtcgatta taacagttta agacccacgg ttgtttatct cccaaaagat attgaattga
     4921 agcataccgt gtggaaagtt gccgaaatga ttcaggttga gcacgatccc gaaaataagc
     4981 tctttgaaaa acgtcctttc gacctgacta aagacaaata cgacggttat atattacaat
     5041 tgccagcagg ctctgaaaat tcaaaaatag aacttgttgt aggtatcacg aaaaaagtgg
     5101 attgcaatcg gcatcgtctt atcgacgtaa ctttcggaga tgccggagac atgaattttt
     5161 ctccccacat gtttatgatt tttgattcgg acggtgaaat tgcgtctacg cggatgggtt
     5221 gcccagacag ggaactgacc gggaaattta tctacgggga aacaagaagg gtaccgtata
     5281 caacagatcc catgattatt tttattccta aaggatttga acttcgctat cggatttggg
     5341 aaagtgttgt ctagaaagta gccttttttc gtgaatgtat tattccagaa gttgttttgt
     5401 ctggttaaac gacggtcact gtctctgttt tctttatccg aatagtgtgc gaatttcagt
     5461 cttttatctt ttttattagc cttagagctt tgtttttagc gtattttatt gtatctttgc
     5521 cgtccgaaaa ataatttttc ggtttaaatc attaataatt aatttatttt agtatgaaca
     5581 attacgaaac cgttttcatt gtaactcccg ttttgtctga tgtacagatg aaggaagcgg
     5641 ttgaaaaatt caaaaacctc ctcacggacc aaggggctga gattgtaaat gaagaagact
     5701 gggggctgcg caagctggct tatcccattg acaagaaaac cacaggtttc tataccttcc
     5761 tggaattcaa tgcc
//
LOCUS       DAOSDR010000205         5766 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrimonas sp. isolate AMR_MDS_1826
            NODE_4976_length_5766_cov_3.894064, whole genome shotgun sequence.
ACCESSION   DAOSDR010000205 DAOSDR010000000
VERSION     DAOSDR010000205.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863355
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrimonas sp. (wastewater metagenome)
  ORGANISM  Petrimonas sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Dysgonomonadaceae; Petrimonas.
REFERENCE   1  (bases 1 to 5766)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5766)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:10:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,720
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 35
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5766
                     /organism="Petrimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4976_length_5766_cov_3.894064"
                     /isolate="AMR_MDS_1826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2023866"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            17..1207
                     /locus_tag="PLI30_08720"
     CDS             17..1207
                     /locus_tag="PLI30_08720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010802415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase
                     family protein"
                     /protein_id="HOI79673.1"
                     /translation="MNLLKEKMMKYDAPQKFKAAGIYPYFRVIESDQDTEVVINGKKV
                     LMFGSNAYLGLTNHPKVKEAAVEATQKYGTGMAGSRFLNGTLDIHIELERKLANFLRK
                     DDAIVFSTGFTVNEGVLGAITGREDYIIWDERNHASIIEGIRVSFSTKFKFRHNDMVS
                     LERQLKRCDPNKVKLIVVDGVFSMEGDLADLPGIVKLARKYNANIMVDEAHGLGVLGR
                     RYNGKGTCDHFGLLPDVDLIMGTFSKSLASIGGFIAGDYHTINYLRHNARTYIFSASI
                     TPAATAAASAALDILRAEPERIKNLWELTHYTIQQFKDRGFEVGPTSTPIIPLYIRSD
                     EKTFAITKQLFEEGIFVNPVVPPAVAPNETLLRFSLMATHTYEQIDYAIDHIEKMFRK
                     FGVI"
     gene            1321..2256
                     /locus_tag="PLI30_08725"
     CDS             1321..2256
                     /locus_tag="PLI30_08725"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006842404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HOI79674.1"
                     /translation="MQVIDLTHRIETELPEVVATLGFFDGVHIGHRHLIEQVKTEADR
                     LGLPSAVVTFPVHPRKVLLADYQPQLLCGYEEKLEQLTATGVDYCISLPFTVELSKLT
                     ASEFITRVLKEKIHVHTMLVGYDHRFGHNREEGIKEYVLYGKAVGMNIIQATEMRVDG
                     EDVSSSRIRRLLMKGEIAKANQLLTYNYTLSGKIVEGYQVGRTIGFPTANVKAWEKYK
                     VVPLLGVYAVLVHFRDKTHQGMLYVGTRPTLQNGTEISVEVNIFDFDGDLYNESLTVE
                     FIDFIRGDEKFESIQQLIDQIYRDKDNVIKRLKNV"
     gene            2259..3083
                     /locus_tag="PLI30_08730"
     CDS             2259..3083
                     /locus_tag="PLI30_08730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007655781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HOI79675.1"
                     /translation="MEYTLLNNGIKIPMLGLGTYRIGKTDNDVYRAIRTALDVGYRHI
                     DTATLYGNEAPIGKAIRESGIQREEIFVTTKLWGDDVMKEAVPQAFDRSMQLLNIGYI
                     DLYLIHWPVKGKLVSTWREMEKIYASGKAKAIGLSNHLQHHLETILEEANIPPAVNQV
                     EMHPYVVLDDLVGFCAENGIICEAWSPLGSNKIPLLKEQVLQEIGERYGKSPAQVVLR
                     WDLQRGVIAIPKSSSQERQQANLDIFDFELTPEEMQFIRSLDRNYRTGIHPDEITF"
     gene            complement(3090..4130)
                     /gene="mltG"
                     /locus_tag="PLI30_08735"
     CDS             complement(3090..4130)
                     /gene="mltG"
                     /locus_tag="PLI30_08735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019541215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HOI79676.1"
                     /translation="MEQKPKRKGRKIFLWAILSLLLIVIAGVVVVRKTIFKPFSLEET
                     VYIYIDNEKNYEEVITQLKKADLPSEQLFRILSERMNYPSNIKTGRYAVKDGMTMPDV
                     IRMLRAGIQTPVKLTFNNVRTKEDLAGRISQQLMMDSTLLVNALNDPEKIKEVGFDEN
                     TLTALFIPNTYEVYWDTSIDHFLNRMKKEYDRFWSDARKSKAEKIGLSPVQVSTLASI
                     VEEEATYSDEYPVVAGLYLNRLKKGMRLEADPTVKFAVGDFSLRRILFRHLEVESPYN
                     TYRNTGLPPGPIRIPSIQAIDGTLSPQQHNYLFMCAKEDLSGRHNFAATHAEHSRNAA
                     RYQRALNERGIF"
     gene            4256..4342
                     /locus_tag="PLI30_08740"
     tRNA            4256..4342
                     /locus_tag="PLI30_08740"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4290..4292,aa:Ser,seq:gct)
     gene            complement(4405..5079)
                     /locus_tag="PLI30_08745"
     CDS             complement(4405..5079)
                     /locus_tag="PLI30_08745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005675863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOI79677.1"
                     /translation="MIKTENLQKIFRTEEVETWALSEVNIEINEGEFVAIMGPSGCGK
                     TTLLNILGLLDNPTSGQYFLNGTDVSKFTENERTNLRKGIIGFVFQSFNLIEELNVYE
                     NIELPLLYMRIPAAERKQRVKEAMNRMAISHREKHFPQQLSGGQQQRVAIARAVVANP
                     KLILADEPTGNLDSKNGAEVMSLLTDLNSEGTTIVMVTHSQHDAGYANRIIKLFDGKV
                     VPEVVL"
     gene            complement(5127..>5766)
                     /locus_tag="PLI30_08750"
     CDS             complement(5127..>5766)
                     /locus_tag="PLI30_08750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOI79678.1"
                     /translation="QVVGIIKDFHTGHLSKTTTPLVISYTERGFHFDYLMARFVPGKQ
                     KEALAYLENLYKEINNDAEFTYSLLEDDIAKLYEEDKRISRVYLLFSIIAILVSCMGL
                     FAISVFDIRQRYREIALRKVNGATTNNIMHLFLNKYLMLLAVSFLTAIPISYVVISKH
                     LENFAYKISVSSWIAMFSVISAIIVIFVSLLTLVWQIKTAMRTNPANALKLE"
BASE COUNT         1565 a         1218 c         1382 g         1601 t
ORIGIN      
        1 ttttaatttt tatctgatga atctgttaaa agagaagatg atgaaatatg atgccccgca
       61 gaaatttaaa gcggcaggca tttatccata tttcagggtt atcgaaagcg atcaggacac
      121 ggaggtagtt atcaacggta agaaagtgtt gatgtttggg tcaaacgctt acctggggct
      181 caccaatcat cccaaagtaa aggaagcggc tgttgaagcc actcaaaaat atggaacagg
      241 tatggctggt tcacgttttt taaacgggac actggatatt catatagaac tcgagcgtaa
      301 gttggctaat tttttaagaa aagatgacgc tatcgttttc tccaccggat tcactgtcaa
      361 cgaaggtgtc ctgggtgcga ttaccggacg agaggattac atcatatggg atgagcggaa
      421 ccacgcctcc attatcgaag gtattcgtgt ctctttctca accaagttca aattccgtca
      481 caacgatatg gtatcgctcg aaaggcaact gaagcgttgc gatcccaaca aggtgaaact
      541 gattgtggta gatggcgtat tcagtatgga gggggacctg gcggatttgc cgggaatcgt
      601 taagctggcc agaaagtata acgccaacat catggtagat gaagcgcacg gactgggagt
      661 tttggggagg aggtacaacg ggaagggcac ttgcgaccat ttcggattac tacccgatgt
      721 ggaccttatt atggggactt tcagcaaatc cctggcctct atcggtgggt tcatcgccgg
      781 ggattatcac acgatcaact acctgcggca caatgcccgg acctatattt ttagcgcgag
      841 cataacacct gctgcgactg ctgctgcatc tgcggccctg gatatcttgc gtgcggaacc
      901 cgaaaggata aagaatttgt gggagctgac gcattacacc attcaacaat tcaaagaccg
      961 cgggttcgag gtagggccta catctacacc gattattccg ctctatatcc gcagcgatga
     1021 gaaaacattt gccattacga aacaactgtt tgaagaaggt atttttgtga atccggttgt
     1081 tccgcctgct gttgcaccca acgaaaccct tctccgtttc tcgttgatgg caacacatac
     1141 atacgaacaa attgactacg ccatagatca tattgaaaaa atgttcagga agtttggcgt
     1201 aatctagcat acagcacaga cagctgtgag acctcgaaat tctgtttcgg ggtcttttat
     1261 tacatgccaa aactaaaagc aacgttcagg cgttaagaat actcacaaaa aggagaaatt
     1321 ttgcaagtca ttgatttaac ccatagaatt gaaacggaac tacctgaggt cgtcgctacc
     1381 ctgggctttt ttgatggagt tcacatcggc caccggcacc tgatagaaca ggtaaaaacg
     1441 gaagctgacc ggctaggtct gccatcagcc gtggtcactt ttcctgtaca tccgagaaag
     1501 gtactgctcg ctgattatca gcctcaattg ctttgcggtt atgaagaaaa actggagcag
     1561 ttgactgcaa caggagtaga ttattgcatc agtctgccgt ttaccgtgga gctgtcgaag
     1621 ttgacggcca gtgaatttat tacccgggta ctgaaagaga agattcatgt ccatactatg
     1681 ttggtgggtt acgatcaccg gttcggacac aaccgggaag agggcatcaa ggagtatgta
     1741 ttatatggca aggctgtagg gatgaatatt attcaggcta cggaaatgcg tgtggatggc
     1801 gaagatgtga gctcttcccg gatcaggcgt ttgttgatga agggtgaaat tgcgaaggcc
     1861 aatcaattgc tgacttacaa ttataccctg tcgggaaaaa tagtggaagg ttatcaggtg
     1921 gggcggacca tcggctttcc gactgccaat gtgaaagcgt gggagaaata caaggtcgtc
     1981 cctctattgg gggtttacgc cgtcttggtt cactttaggg ataaaacaca tcagggaatg
     2041 ttgtatgtcg gtacgcgccc gaccttgcaa aacggaacgg agatcagtgt tgaagtgaat
     2101 attttcgatt tcgacggaga cctgtacaac gagtcgttga cggttgaatt tatcgatttc
     2161 atccgtgggg atgaaaaatt tgaaagtatc caacagctta ttgatcaaat ataccgggat
     2221 aaggacaatg tgataaaacg gttgaaaaac gtataaacat ggaatatact ttattgaata
     2281 acggtatcaa aatacctatg ctgggtctag gaacctacag aattgggaaa acagataatg
     2341 atgtttatcg tgctataagg acggctttgg atgtaggtta cagacatatt gacacggcaa
     2401 ctttatacgg caacgaagct ccgatcggca aagccattcg tgagagcgga atacagcgtg
     2461 aagagatttt tgttaccacc aaactttggg gggatgatgt gatgaaagaa gctgttccgc
     2521 aggctttcga caggagcatg caactgttga atatcggcta catcgatctt tatctgatcc
     2581 actggcccgt aaaaggaaag cttgtttcca cctggcggga aatggagaaa atctacgctt
     2641 cggggaaagc gaaagccatt ggattaagca accacctgca acatcatctt gaaacaattc
     2701 tggaagaggc aaacatccca ccggctgtga accaggtcga gatgcatcct tatgtggtgt
     2761 tggatgattt agtcggtttc tgtgcggaaa acgggatcat ttgtgaagcc tggagcccgt
     2821 tgggaagcaa taagatccct cttctcaaag agcaggtatt gcaggaaata ggggagaggt
     2881 atggaaagtc acccgctcag gtcgtgttga ggtgggatct tcaacgtgga gtgattgcta
     2941 ttcccaaatc gtccagccaa gaacgacagc aagcgaacct ggatattttt gattttgaac
     3001 tcaccccgga agaaatgcag ttcatccggt cactcgacag aaattaccgg acgggtatcc
     3061 accccgacga gataactttc tgaggtgttt taaaagatac ccctttcatt gagcgcccgc
     3121 tggtaacgcg ctgcattgcg cgaatgctct gcgtgagtgg ccgcaaaatt atgcctgccc
     3181 gacagatctt ctttagcgca cataaaaaga taattgtgct gttgtggaga caaggttccg
     3241 tcaatagcct ggatggatgg tattcggatt ggcccgggtg gtaatccggt attcctgtag
     3301 gtgttataag gtgattccac ttccaggtga cggaacaaga tacgccgcaa cgagaaatcg
     3361 ccaacggcaa atttcaccgt cggatcagcc tcaagccgca tccctttttt taaccggttg
     3421 agatacaatc cggccacaac cggatattcg tcggagtagg tggcctcttc ttccacaatc
     3481 gatgccagtg tagaaacctg caccggtgat aatccgattt tttccgcttt acttttccgt
     3541 gcgtcgctcc agaagcgatc gtattccttt ttcatccggt tgaggaaatg gtcaatgctt
     3601 gtgtcccagt acacttcata ggtgttgggg ataaacaggg ccgtcagcgt gttttcgtca
     3661 aacccgacct cttttatttt ttccgggtca ttcagtgcgt ttaccaatag ggtgctgtcc
     3721 atcatcagtt gctgtgagat tctaccggcc aggtcttcct tggtacggac attgttgaac
     3781 gtgagtttta ccggtgtttg aattccggca cgcagcatgc gaatcacgtc gggcatggtc
     3841 atcccgtctt taacggcata tcttccagtt tttatgttgc tgggatagtt catcctttcc
     3901 gacaagatcc tgaagagctg ctccgacggc aggtccgctt tttttaattg ggtgattacc
     3961 tcttcgtagt tcttctcgtt atcaatataa atgtacaccg tttcttccag gctgaatggc
     4021 ttgaaaatgg tttttctgac aacaactacc cctgcaatta ctatcagcaa cagggaaagt
     4081 attgcccata gaaaaatttt tctgcctttt ctttttggtt tttgctccat acgtccaaaa
     4141 gtgatttgaa aaactgcaca aatgtaggaa gatttaaata gaaatctccc gttttttttg
     4201 acaagatgaa aacaatcttg tatatttgca cccaatttgg tcggaagtcc taccgggaag
     4261 tgtgggtgag tggctgaaac caccagtttg ctaaactgac gtacgggtaa ctgtaccggg
     4321 ggttcgaatc cccccgcttc cgctaagaaa gaaaatccct tgcaaataaa taatttgcaa
     4381 gggattttct tttaaggaat atgactacaa caccacttct ggtaccactt tgccatcaaa
     4441 caatttaatt atgcggttgg cataaccggc atcgtgttgg ctgtgcgtaa ccatcacaat
     4501 ggttgttcct tcactattca aatcagtgag caaactcatc acttctgcgc cgttcttact
     4561 gtccaagttt ccggtaggtt cgtcagcaag gataagcttg ggattggcga ctacggcacg
     4621 agcaatggca acgcgttgtt gctgaccgcc cgaaagttgc tgcggaaaat gtttctcccg
     4681 atgcgaaatg gccatacggt tcattgcctc tttcacccgt tgttttcgct cggcggccgg
     4741 gattcgcata tataagagtg gaagctcgat gttttcgtac acgttgagtt cctcgatgag
     4801 gttgaaactt tggaacacga aaccaataat acctttccgc aagtttgtcc gttcgttttc
     4861 agtaaattta gatacgtcgg ttccgttgag gaaatactgt cccgatgtgg ggttgtccag
     4921 caaacccaag atattgagta acgtggtttt tccgcagccc gacggcccca taatggcaac
     4981 aaattcacct tcgttgattt cgatgtttac ttcggacaat gcccaggttt ccacttcttc
     5041 cgtgcggaag attttctgta agttttctgt tttaatcata gtttttttta attataaatt
     5101 attaattacc aattgtgtct ttgcgattat tccaacttta gtgcattcgc gggatttgtt
     5161 ctcatggcag tctttatttg ccacacgagg gtaagcagcg atacgaaaat tacaataatt
     5221 gccgatatca cggaaaacat cgctatccat gatgaaacgg aaattttata agcgaaattt
     5281 tcgaggtgtt tgcttatcac aacatatgag atcggaatag cggttagaaa agatacggct
     5341 aatagcatca ggtatttatt cagaaacaga tgcatgatat tgtttgtcgt agcaccgttt
     5401 acttttcgca acgcaatttc gcggtaacgt tgccgaatat cgaaaaccga aattgcaaat
     5461 aatcccatac aggaaaccag aatagctatg atggaaaata acaggtaaac gcgacttatc
     5521 cgtttgtctt cttcataaag tttggcaata tcgtcttcca gtaggctgta cgtaaattct
     5581 gcgtcgttgt taatttcctt gtataaattt tccaaataag ctaaagcttc tttctgtttt
     5641 ccgggaacga aacgtgccat taggtaatcg aaatggaaac cgcgttcagt gtaagaaatt
     5701 actaatggtg tggtcgtttt ggataaatgt ccggtatgaa aatcctttat tatacccact
     5761 acctga
//
LOCUS       DAOSDR010000206         5762 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrimonas sp. isolate AMR_MDS_1826
            NODE_4982_length_5762_cov_4.150692, whole genome shotgun sequence.
ACCESSION   DAOSDR010000206 DAOSDR010000000
VERSION     DAOSDR010000206.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863355
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrimonas sp. (wastewater metagenome)
  ORGANISM  Petrimonas sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Dysgonomonadaceae; Petrimonas.
REFERENCE   1  (bases 1 to 5762)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5762)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:10:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,720
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 35
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5762
                     /organism="Petrimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4982_length_5762_cov_4.150692"
                     /isolate="AMR_MDS_1826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2023866"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1173
                     /gene="nrfA"
                     /locus_tag="PLI30_08755"
     CDS             <1..1173
                     /gene="nrfA"
                     /locus_tag="PLI30_08755"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018667337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-forming cytochrome c nitrite reductase"
                     /protein_id="HOI79679.1"
                     /translation="AFSKDYGTPRGHMHAVEDIHHTLRTGAPMSSDEGPQPATCWTCK
                     SPDVPRLMDSLGIAEYYKGTWASLGQEVVNPIGCADCHEAETMNLKISRPGLIEGFRN
                     SGQDIHKVSQQEMRSLACAQCHVEYYFSPEGKYLIFPWHNGLTVEGGEQYYDSIRFAD
                     YTHKLSRAPIIKAQHPDYEIYKTGIHAQRGVSCADCHMPYVSEGGVKYTSHHVRSPLA
                     SINNTCQVCHRESEETLRTAVFERQRSANEIRNRVEKELATAHIEAKFAWEKGATEAQ
                     MEEVLQLLRQSQWRWDYAVASHGGSFHSPVEFQRILSMSLDRAHKARFVLSKVLAQLG
                     YIGDVPMPDISTKEKAQAYIGLDMRQEREAKKQFMETVVPKWLETAKANNRLVSRR"
     gene            1196..2470
                     /locus_tag="PLI30_08760"
     CDS             1196..2470
                     /locus_tag="PLI30_08760"
                     /inference="COORDINATES: protein motif:HMM:NF016995.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein ResB"
                     /protein_id="HOI79680.1"
                     /translation="MNKDARRIWEKPWSYTEGFIVAGGIALTGLLLQLLLGNIRPADF
                     RSPVNTTVGALLVAGLLCCHFLLRDNHVVRWLSGARSTVPAMLVVLLSIVILGLTPQF
                     TIHEPQEHLPNNIFSRMGWYRMTTSWAFVLLCFYILVILGLTVLRKTRKSQSWRLIGF
                     YLNHLGLFFALGGGLLGSGDMQRYTMTVVEGNTEWRAHDALGNLKELPVAIELDTFII
                     EEYPPKLVVIDNRTGKILPVNRPGSYMFEGLGKPTQLNGNTIEVLEYIPQAAVVRDSA
                     FTSVVPMLMDGAATALKVRVTNPSLKAPVEGWVSNGSYLFPYQVLYIDETSSVAMPVQ
                     EVKKYTSQVTVFTEAGHRKKANIEVNKPLSIEDWIIYQYSYDEKMGKYSKTSVFELVR
                     DPWLPVVYTGIFMLLAGALFLFIAGPKKNRKS"
     gene            2478..3272
                     /gene="ccsA"
                     /locus_tag="PLI30_08765"
     CDS             2478..3272
                     /gene="ccsA"
                     /locus_tag="PLI30_08765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005822870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
                     /protein_id="HOI79681.1"
                     /translation="MTWDHFIFFAIPSVLLWTVASAWALRRPKKTLPALFTVLGLGVF
                     FSFILGMWISLERPPLRTMGETRLWYSFFLPVAGLITYQRWGYKWILSFSTILSAVFI
                     VVNIAKPDIHNKVLMPALQSPWFAPHVIIYMFSYAILGAVTLVALYYLFREEKLQDKE
                     RIMDMTDNLVYVGVAFVTMGMLMGAIWAKEAWGHYWSWDPKETWAAATWLGYLVYIHY
                     RLLKTSKNKTSMTILLLAFMLLQVCWYGVNYLPSARGRSVHTYSVR"
     gene            complement(3280..4266)
                     /locus_tag="PLI30_08770"
     CDS             complement(3280..4266)
                     /locus_tag="PLI30_08770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019539620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HOI79682.1"
                     /translation="MQVLHNRISNEELRARLFAEKEPRVTVSLYKYFTIVNPRAFRDE
                     VYVKFNQLNVFGRIYIAHEGINAQISVPESNFNAFRETLYQLSPELDGLRLNIAIDDD
                     GKSFWVLRCKVRSRIVADGITDPGFDASKIGKYLKADEVNEMIGRDDTVFVDMRNHYE
                     YEVGHFENALEVPSNTFREQLPMAVEMLKENKDKNIVMYCTGGIRCEKASAYLRYKGF
                     PHVFHVEGGVIEYARKAREQCLPLKFIGKNFVFDERLGERITDDIIAQCHQCGKPCDN
                     HTNCNNDGCHLLFIQCDECKNKYDGCCSDECKEEFHLPEEEQQRARRAGRVN"
     gene            complement(4359..5117)
                     /gene="truA"
                     /locus_tag="PLI30_08775"
     CDS             complement(4359..5117)
                     /gene="truA"
                     /locus_tag="PLI30_08775"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006841543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HOI79683.1"
                     /translation="MKRFFITLSYNGKNYVGWQIQPNGVSVQQVLQDALSTVLREPVE
                     VVGAGRTDAGVHARKMVAHVDFSDERFDSMRLVKNLNSFLPHDISIREIREVSPDAHA
                     RFSAISRTYKYYLTTEKNPFLYDLTYRVFFQPDIDTMNALCEVLKGYEDFTSFSRLHS
                     DVKTNNCRISEAYWEQEDTTRFTFTITADRFLRNMVRAIVGTLLEAGRGKLDERGFRR
                     IIEARNRQVAGDSAPGHALFLHEVVYPEDIWVAK"
     gene            complement(5119..>5762)
                     /locus_tag="PLI30_08780"
     CDS             complement(5119..>5762)
                     /locus_tag="PLI30_08780"
                     /inference="COORDINATES: protein motif:HMM:NF039204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF5715 family protein"
                     /protein_id="HOI79684.1"
                     /translation="CCGKGKAETTETAETVKTARGIPLERKQRFSGNYNKEFNDLQDA
                     HLSAATALGISPMALRNDTLKLKDELVRLPDELELYKMYDLTHSIPFLVPSAAQLFVD
                     ITRNFRDSLYSKDLPLYRLYLTSILRTDEDLSILTKRNINASENSTHRYGTTFDISWK
                     RFDPVMPDSIRQVPPERLKLVLAQVLFDLKNQQRCCVKHERKQACFHITARGK"
BASE COUNT         1498 a         1421 c         1357 g         1486 t
ORIGIN      
        1 gctttctcga aagactatgg aactcccagg ggacacatgc atgcggtaga agatatccac
       61 cacacgttac ggacaggtgc acccatgtct tccgatgaag gtccgcagcc ggcaacctgc
      121 tggacttgta aaagtcccga tgttccccgc ctgatggatt cgctaggaat tgccgaatat
      181 tacaaaggca cgtgggcctc gttgggacaa gaagtggtaa accccatcgg ttgtgccgat
      241 tgtcacgagg ctgaaaccat gaaccttaaa atcagccgtc cgggactgat cgaaggattc
      301 agaaacagtg gccaggacat ccacaaggtt tcgcaacagg aaatgcgttc gctggcttgc
      361 gcccaatgcc acgtggagta ttatttctct cccgaaggga aataccttat tttcccctgg
      421 cacaatgggt tgaccgtgga aggtggtgaa caatattacg acagcatccg gtttgccgac
      481 tatacccata agctcagccg cgcccccatc attaaagcac aacatcccga ttacgaaata
      541 tacaaaaccg gcattcatgc ccaacggggt gtttcgtgtg ccgattgcca catgccttac
      601 gtatcggaag gcggcgttaa atacaccagt caccacgtaa gaagtccgtt ggcaagcatc
      661 aacaacactt gccaggtttg ccaccgtgaa agcgaagaga ccttacgtac cgcagtcttc
      721 gagcgtcagc gcagcgctaa cgaaatccga aaccgggttg aaaaggaact ggcaaccgcc
      781 cacatcgaag caaaatttgc ctgggagaaa ggggctacag aagcacaaat ggaagaggtg
      841 ctgcaacttc ttcgtcaatc gcaatggcgt tgggattatg ccgtagcctc gcatggcggt
      901 tcttttcact ctccggttga attccagcgg attctctcca tgagtctcga ccgtgcccac
      961 aaagcccgtt tcgtcctctc taaagtattg gcgcaactcg gctacatcgg cgatgtcccc
     1021 atgcccgaca tctccaccaa agaaaaagca caagcttata tcggtctgga catgcggcag
     1081 gaaagagaag caaaaaagca attcatggaa acggttgtcc ccaaatggct ggaaacagct
     1141 aaagccaata accggttggt gagcagaaga taaaacggca tcgtcacggg taattatgaa
     1201 caaagacgct cgaagaatat gggagaaacc gtggagctac accgaaggat ttatagttgc
     1261 cggaggcatt gcactgacag gactcctgct gcagctcctg ctcggcaata ttcgccccgc
     1321 tgatttccgt tcccctgtaa ataccaccgt cggggccctg ctggttgcag ggcttctttg
     1381 ttgccacttt ctactgagag acaatcacgt tgtccgctgg ttgtcgggcg caaggtcaac
     1441 cgttccggct atgcttgtgg tgctgttgtc tatcgtcatt ctggggctga cacctcagtt
     1501 cactatacac gaaccgcagg aacatctgcc gaacaacatt ttcagccgga tgggatggta
     1561 ccggatgaca acttcatggg cattcgtgct tctctgtttt tacatcctgg taattctagg
     1621 actcacagtg ctgagaaaaa cccgcaaatc tcaatcgtgg aggctgatcg gattctacct
     1681 caaccaccta ggattgtttt ttgctctggg gggcggcctg ttgggaagcg gcgacatgca
     1741 acggtacacc atgacggttg tggaagggaa cacagaatgg cgtgcacacg atgctctggg
     1801 aaacctcaag gagcttcctg tagctattga actggatacg tttataatag aagaatatcc
     1861 accaaaactg gttgtcattg ataatcgcac gggaaaaatc ctgcccgtaa accgtcccgg
     1921 atcgtacatg tttgaagggt tgggcaaacc aactcaacta aacggaaata ctatcgaagt
     1981 gctcgaatat attccgcaag cggctgtagt aagggactct gcctttacaa gtgttgtacc
     2041 catgcttatg gacggagcag ccactgcctt gaaagtacgg gtaaccaacc cgtctttaaa
     2101 agcacctgtt gaaggctggg taagcaacgg aagttatttg tttccctatc aggtattgta
     2161 catcgatgaa acctccagtg tggcaatgcc cgtgcaggaa gtgaagaaat acacttcaca
     2221 agtcactgtt ttcacggaag ccggacacag gaaaaaagcg aatatcgaag tgaacaaacc
     2281 tttgtcaata gaagactgga ttatctacca gtacagttac gacgaaaaaa tgggcaaata
     2341 ttccaaaacc agtgttttcg aactcgttcg agacccctgg ttaccggtag tttacaccgg
     2401 tattttcatg ttgctggccg gagcactgtt cctgtttatt gcagggccta aaaaaaatcg
     2461 taaatcataa attgaacatg acctgggacc attttatctt ttttgccata ccatcagttt
     2521 tgctttggac agtagcgtcg gcatgggcac taagaagacc taaaaaaacg ctaccggctt
     2581 tgtttacggt tttaggcctg ggagtgttct tctcgtttat cctcggtatg tggatatccc
     2641 tggaacggcc gccgttgcgc acgatgggtg aaacccggtt gtggtattcc ttttttctgc
     2701 cggttgccgg actgataact taccagcgtt ggggatacaa atggatatta tcgttcagca
     2761 ccatcctttc ggcggttttt atcgttgtga acatcgctaa acccgatatc cacaataagg
     2821 tgttgatgcc tgccctgcaa agtccgtggt ttgccccgca cgttattatc tatatgtttt
     2881 cttacgccat tctgggagcg gtaacattgg tagccctgta ctacctgttc cgggaggaaa
     2941 aactccagga caaagaacgt atcatggata tgaccgacaa cctggtgtat gtgggtgttg
     3001 cttttgttac gatgggcatg ctaatggggg cgatctgggc aaaagaagcc tggggacatt
     3061 actggagctg ggatccgaaa gagacgtggg cagccgctac gtggctcgga tacctggtct
     3121 acatccatta ccggctcctg aaaacatcga aaaataagac atcgatgact attctccttt
     3181 tggcctttat gctgcttcag gtatgctggt acggagtcaa ttacctgcca tctgcccggg
     3241 ggagaagcgt gcatacctac tctgtaagat aagtgggagt taattgactc ttcctgcacg
     3301 acgtgcccgt tgttgttctt cttcgggcaa atgaaactct tccttacatt catcactgca
     3361 acagccgtcg tacttgtttt tacactcatc gcactgaata aacaacaggt gacatccgtc
     3421 gttgttacaa ttggtatggt tgtcgcaagg cttaccacat tgatggcatt gagcgataat
     3481 atcatcggta atccgctctc ccaaacgctc atcaaacacg aagttttttc cgataaattt
     3541 caacggcaaa cattgttccc tggctttacg ggcatattct atcacaccgc cttctacgtg
     3601 aaatacgtgg ggaaagcctt tgtaacgcag gtaagcgctg gctttctcac accgtatgcc
     3661 tccggtacag tacatcacga tatttttatc cttgttttct ttcaacattt ccacagccat
     3721 aggcaattgt tcgcgaaaag tattggaagg gacttccagt gcattttcaa aatgccctac
     3781 ctcgtattca taatggttac gcatgtccac aaaaacagta tcgtcacgtc cgatcatttc
     3841 attgacctca tctgctttca ggtatttacc aatttttgac gcgtcgaagc cgggatcggt
     3901 gatgccatcc gctacaatcc tggaacggac tttacaccgt aacacccaga atgatttacc
     3961 gtcatcgtca atagcaatgt tcaaacgaag gccatcaagc tcaggcgaaa gctgataaag
     4021 ggtttccctg aaggcgttaa aattactttc aggcacactt atttgtgcgt taatcccttc
     4081 atgagcaatg taaatccttc cgaaaacatt caattggttg aatttcacat atacttcatc
     4141 gcggaatgct cgcggattaa ctattgtaaa atatttgtac agagaaacgg taacacgcgg
     4201 ctccttttct gcaaacaggc gtgctcttaa ttcctcatta gatattcggt tgtgtaacac
     4261 ttgcatggtg aacgctcctt ttaaataatg aaaacaataa tcgctgcaaa tttaataaaa
     4321 aaatggtctg ataactattt ccggcttcaa gcagtggcct acttagccac ccagatgtcc
     4381 tcaggatata ctacttcgtg aaggaacaac gcatgtccgg gagcagaatc accggcaacc
     4441 tgacggttcc tggcttctat aatccgtcga aacccacgct cgtccagttt tccccggccg
     4501 gcttcgagca atgttcccac gatggcgcgg accatattgc gcaggaatcg gtcggcggtg
     4561 atggtaaaag taaaacgggt ggtatcttcc tgttcccagt aagcttcgct tatacggcag
     4621 ttgttggttt tcacatcgct atgcagccgg ctgaagctgg taaagtcttc gtatcctttc
     4681 aacacctcac acaaggcatt catggtgtct atatcgggtt ggaaaaacac ccggtaagta
     4741 agatcgtaca gaaacgggtt cttttccgtc gtgaggtaat atttatacgt ccgggatatg
     4801 gctgaaaagc gggcatgtgc gtcggggctc acttcccgga tttcgcgaat ggatatatcg
     4861 tggggcagaa agctgttgag gtttttcacc aacctcattg aatcgaaacg ctcatccgaa
     4921 aaatcaacat gcgccaccat cttccgggca tgcacacccg catcggttct gcccgctccc
     4981 accacttcca ccggttcacg taaaacggta gaaagggcat cctgtagcac ctgctgcaca
     5041 ctgactccgt tgggttgtat ttgccagccc acatagtttt tcccgttata cgaaagtgta
     5101 ataaaaaagc gcttcatctt actttcccct tgccgttata tggaaacaag cctgcttccg
     5161 ctcgtgtttc acgcaacacc gttgttgatt ttttaagtcg aaaagtacct gcgccaacac
     5221 aagcttcaac cgttccggcg ggacctggcg aatggaatct ggcatcaccg ggtcgaaacg
     5281 tttccacgat atgtcgaagg tagttccgta acgatgtgtg gagttttcac tggcgttgat
     5341 attccttttt gtcaatattg aaaggtcttc atcggtgcgg aggatactgg tcaggtaaag
     5401 ccggtaaaga ggtagatctt tgctgtaaag cgaatccctg aaattccgcg taatatcaac
     5461 aaacaactgc gctgctgaag gaaccagaaa cggaatagaa tgggttaaat cgtacatttt
     5521 gtaaagttcc agttcatccg gcaatcggac aagctcatct ttcagcttca acgtatcgtt
     5581 gcgtaaagcc atcggtgaaa tacccaaggc agtagcagcc gacaagtgag catcctgtaa
     5641 atcgttgaac tccttgttgt aattccccga aaaccgttgt ttccgctcca acggaattcc
     5701 acgtgccgtc ttgacggttt cggcagtttc ggtggtctcc gctttacctt ttccacagca
     5761 ca
//
LOCUS       DAOSDR010000207         5758 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrimonas sp. isolate AMR_MDS_1826
            NODE_4986_length_5758_cov_3.810100, whole genome shotgun sequence.
ACCESSION   DAOSDR010000207 DAOSDR010000000
VERSION     DAOSDR010000207.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863355
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrimonas sp. (wastewater metagenome)
  ORGANISM  Petrimonas sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Dysgonomonadaceae; Petrimonas.
REFERENCE   1  (bases 1 to 5758)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5758)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:10:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,720
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 35
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5758
                     /organism="Petrimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4986_length_5758_cov_3.810100"
                     /isolate="AMR_MDS_1826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2023866"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..518)
                     /locus_tag="PLI30_08785"
     CDS             complement(<1..518)
                     /locus_tag="PLI30_08785"
                     /inference="COORDINATES: protein motif:HMM:NF024181.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SusD/RagB family nutrient-binding outer membrane
                     lipoprotein"
                     /protein_id="HOI79685.1"
                     /translation="MKKFLKYASLLSVVVLMAACQDFLDVNEDPNNPVSVSPDLVLPT
                     AQVYTANVMHNNRYLNNLGNMMMYNWSQSDGYAWYPDEFKYNVTSSFYQQIFNYSYSN
                     TLKMYHVLDQLDSTYSYYRAIGKIMKAYHFQLLVDCYGNIPYSEALKRSGEATPKYDD
                     AKTIYEDLVVQLT"
     gene            complement(532..3636)
                     /locus_tag="PLI30_08790"
     CDS             complement(532..3636)
                     /locus_tag="PLI30_08790"
                     /inference="COORDINATES: protein motif:HMM:TIGR04056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0071702 - organic substance transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SusC/RagA family TonB-linked outer membrane
                     protein"
                     /protein_id="HOI79686.1"
                     /translation="MKKVALLLALIFIGLQVFSQTREITGTVTSAEDGGAIPGVSVLV
                     KGTTLGTVTDLEGRFRLQVPNNAQTLVFSFVGMTSQEVAIGNQNTFRIQLKAETIGVD
                     EVVVTALGIKREKREVTYQTQKVDQGELTKIAPTRVASALAGKVAGLQINVQDNGVNP
                     SSQILLRGMRSITGDNSALIVIDGSIASQGAFDALNPSDIADISVLKGATAAALYGSS
                     AGNGALIVTTKAGQASRKFIVGVNASFTMDKVAYMPDFQTEYGTGWEGAYDPVENTNW
                     GPRFDGTLRQIGPTFPEGYGLETQVVPYAPVKDNLLHFFQTGQTFNNTVYLTGSSDNS
                     SYYISVGDQRAEGIVLDDTYRRNTFRVNATKKLGKLELSATTSYFTDKTDVVGSTIGD
                     QDRPLYWFILNTSANIPLEKYKDWQNPKSYGYADNYYNAYYQNPYWAIGTNRNIDETN
                     RLNGNINLSYDILDWLNFTARASVNNVWGNGKNWRAAQTYNEELQPAHSAVSSFVEDS
                     EFQTKTYTGDALVTGSFKFYDSFSLKAILGSTVYSTDYRGSTIRANNLSIPGFYDISN
                     GTGKLEGTVDESLKRYYGFFGDFTLGYKNYLFLNFSGRNDWTSTLAKGSNSYFYPAAG
                     LSFVVTDAINALKNNNILSYAKVTFSNSTVYNDLAPYRINEKYYQHVSFPFGTVNGFY
                     LDPAAVDASITKEKINTTEVGLNLGFFQSRVWLDASFFNTITSDLITATTPSVSSGST
                     SFLTNIGELQGKGIELTLGGSILKTKDFKWDVNINYTSNETVVNEIYEGLDEVAITTT
                     GQYGVYAVVGKAFPQMKANVYKRDPQGRIIVDPASGHPLTETGLANLGKTIPDYIVGL
                     SSSATYKGFTVSATFDYRTGHVYYEQGSDQMEFTGRSKESVSANRQDFVIPNSVIETS
                     PGVYVENTNIPVSGGRQSYWTDVYNDVKSNYVKDATALKLRELAVNYELPRNLISKTP
                     FQKISFGFIGRNLLTWLPAENRFSDPEFNNTRSTDPANAVGVGGYLQSPPTKSFGFNL
                     NVEF"
     gene            complement(4141..5697)
                     /locus_tag="PLI30_08795"
     CDS             complement(4141..5697)
                     /locus_tag="PLI30_08795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005925906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PglZ domain-containing protein"
                     /protein_id="HOI79687.1"
                     /translation="MRKIRILWTDDEIDLLRPHIIFLREKGYEVETANNGSDALDWVK
                     KEHFDIIFLDENMPGLSGLETLSQIKTISPNVPVVMITKSEEENIMDEAIGSKITDYL
                     IKPVNPKQILLSLKKNVEQKRLVTQQTTSAYQMQFAQIGMRINDRLSFEEWKEIYRKL
                     VHWELELSSSEDSAMDEVLAMQKNDANQAFCRFIRENYLHWFGKPLGQRPLLSTDIFR
                     QKVFPLLNNGRKVFVLLIDNLRFDQWRVMSSVVSEYFRTDEEELYYSILPTATMYARN
                     AMFAGLMPSEIEKLYPNLWEDDDNEGNKNEHEEELLLKQMLRLGRKEKLFFDKISNLK
                     SGRKLVEDLSAILENDLSVMVFNFVDMISHARTEMDMIRELAGNENAYRSLTLSWFNH
                     SSLLELLKELAGNDIRVVITTDHGTIRVDNPVKVIGDRETNTNLRYKLGRNLNYKSRQ
                     VFDVTNPRDAFLPSRNVSTTYIFAQNTDFFAYPNNFNYYANYFKNTFQHGGVSLEEMI
                     VPVVTLSPKK"
BASE COUNT         1654 a         1314 c         1094 g         1696 t
ORIGIN      
        1 gttaattgaa caaccaaatc ctcataaata gttttagcat catcatactt cggagtagct
       61 tcaccacttc tttttagcgc ttcgctgtaa ggaatattac cgtaacaatc gaccaataac
      121 tgaaagtgat atgccttcat gattttccct attgctctat aataagaata ggtactatcc
      181 agctgatcta gcacatgata cattttcagc gtattggagt aagaatagtt aaaaatttgc
      241 tgataaaacg atgaagttac attgtactta aactcatcag gataccaggc ataaccatca
      301 ctttgactcc agttatacat catcatattc cccagattgt tgagatatct attgttatgc
      361 ataacgttgg cggtgtaaac ctgagctgta ggcaatacca aatcaggaga aaccgacacc
      421 ggattgttag gatcttcatt cacatccaga aaatcctggc aagctgccat cagtactaca
      481 accgataaca aactggcata tttcagaaac tttttcatct ttcaattttg tttagaattc
      541 gacattcagg ttaaatccaa atgattttgt cgggggagac tgaagatatc cacccacccc
      601 aaccgcattc gcaggatcag tgcttctggt attattaaat tcagggtcag agaatctgtt
      661 ttctgcaggt aaccaagtca aaagatttct tccaataaaa ccaaaactga ttttctgaaa
      721 aggtgttttg ctaattaaat tcctgggcag ttcataatta acggccaact cccttaactt
      781 aagagcagta gcatctttca catagttgga cttcacatcg ttgtaaacat cggtccaata
      841 gctttgacgc ccgccagaaa ccggaatatt cgtattttcc acgtaaactc ctgggctggt
      901 ttcaataaca gaatttggga tcacaaaatc ctgccggtta gccgaaacac tctcttttga
      961 ccgtccagta aactccatct gatctgagcc ttgttcgtaa tatacgtgtc ccgttctgta
     1021 atcaaaagtt gctgaaacag taaatccctt ataggttgca gaagaactca gccctacaat
     1081 ataatccgga atagttttgc ctaaatttgc caggccggtt tctgtcaatg gatggccgct
     1141 agcgggatcc acaattattc ttccttgcgg gtcacgctta taaacattgg ctttcatttg
     1201 agggaaagcc ttgccaacca ctgcataaac accatattga ccagtagttg taatagcaac
     1261 ctcatccaat ccttcgtaaa tttcatttac gactgtttca tttgaagtgt aattaatgtt
     1321 tacatcccat ttgaaatctt tcgttttcaa aattgaccca ccaagggtca attctatacc
     1381 tttcccctgc aattcaccta tgttcgtgag aaatgaggtt gagcctgacg aaacagatgg
     1441 agtagttgct gtaatcagat cggacgtaat ggtgttaaaa aaagaagcat ctaaccagac
     1501 acgactttga aagaatccca aatttaatcc aacctcagta gtgttaatct tctctttggt
     1561 aatgcttgca tcaacagctg ctggatcaag ataaaagcca tttaccgtac caaacggaaa
     1621 agatacatgc tgataatatt tttcattaat ccgataaggc gccaagtcgt tgtaaactgt
     1681 agaattacta aaggtcacct tagcatatga caaaatattg ttgtttttta atgcattaat
     1741 ggcatccgta acaacaaatg acaaaccggc tgccggataa aaatagctgt tactcccttt
     1801 agcgagtgtt gaagtccagt cgtttcttcc ggagaaatta aggaataaat aatttttata
     1861 ccctaatgta aaatcgccaa agaacccata atatcttttt aatgattcat caacagtccc
     1921 ttccagtttt cctgttccgt tcgagatatc atagaaacca ggaatactta aattattagc
     1981 cctgatcgtg cttccgcgat agtcagtaga ataaaccgta ctcccaagaa ttgcttttaa
     2041 tgaaaacgag tcataaaact taaaacttcc ggttactagg gcgtcgccgg tgtaagtttt
     2101 agtctggaat tcactgtcct ctacaaaaga cgaaacagct gaatgcgctg gctgcaattc
     2161 ttcgttatag gtttgtgctg cacgccaatt cttaccattc ccccaaacat tgttgacact
     2221 ggcccgtgca gtaaaattta accaatccag aatatcgtag gataggttaa tattcccatt
     2281 caaccgattg gtttcatcaa tatttctgtt tgtaccgatt gcccaatatg ggttctgata
     2341 atacgcattg taataattgt cggcataacc ataacttttg ggattctgcc agtctttgta
     2401 tttttcaagt ggaatattag ccgaagtgtt cagaataaac cagtagagag gcctatcttg
     2461 gtcgccaatt gtacttccca ccacatcagt tttatcggta aaataactgg tagttgcaga
     2521 cagttctagt ttcccaagct tttttgtagc attgacccta aaagtatttc tgcggtaagt
     2581 atcatccaac acaatccctt ctgctcgttg atccccaaca gaaatataat aactgctgtt
     2641 gtcactgctt cctgtcaaat atacggtatt gttaaaagtt tggccagttt gaaaaaaatg
     2701 aagcaagttg tctttaacag gggcatatgg tacaacttgg gtctctaaac catacccttc
     2761 cggaaaagta ggtccgatct gacgaagggt cccatcgaat cgtggccccc aattggtgtt
     2821 ttcaaccgga tcgtaagccc cctcccatcc agttccatat tctgtctgaa aatcaggcat
     2881 ataggcaacc ttatccatgg taaaagaagc gttgactccg acaatgaatt ttctactagc
     2941 ttgaccagct ttggttgtaa caattaaggc cccattacca gcgctcgaac catacagtgc
     3001 cgcagcggtt gctcctttaa gtacgctgat atctgcgata tcgctaggat tcaacgcatc
     3061 aaaagcccct tgtgaggcaa ttgagccgtc aataacaatt aatgcagagt tatctccagt
     3121 aatggaacgc atccctctca acaatatctg tgaacttgga tttaccccat tatcctggac
     3181 atttatctgc aatcctgcaa ctttgccggc aagagcactg gcaactcttg taggagcaat
     3241 cttagtcaac tctccctgat ctaccttttg tgtttgataa gtaacttcac gtttttccct
     3301 tttaatcccc aaagctgtca cgacaacttc atccaccccg atggtttctg ctttcaactg
     3361 gattctgaag gtgttctgat ttccgatggc aacttcctgg gaggtcattc caacaaaaga
     3421 aaagacaagc gtctgagcat tattgggaac ctgcagtcga aatcgaccct ccaggtcggt
     3481 aaccgtcccc aatgtcgtgc ctttcaccaa aacagatacc ccgggaatag ctcccccgtc
     3541 ttctgccgaa gtcacggtac ctgtaatttc cctggtctgt gaaaagacct gaagccctat
     3601 aaaaataagg gctaacaaca gcgcaacttt tttcatagaa atagttttaa gtttgcaaat
     3661 taaacaacat gtagtattta ctataaaatc aaaacccatc ctcagttatc cgtaatcttc
     3721 tcaaggcccc tcttcctttc gaaaaccaca cccaataagg cttttttaaa tcttgcaatt
     3781 taaaaaatga tactcttttg gaataaaaat ttagatttta taactaacac aaatttaaaa
     3841 aaagaaatta ataagcaaac attcgcaggc tttttgtgtg gctttatatt attataaaga
     3901 gaggtgaaat ttctacgcct tacaaacaac taacaaacaa caagttaaca cttttttgtt
     3961 ttttgtgtta atttttgttt caaaaatcgt cgacctgctc aaaaaaaact tcaggaaatt
     4021 attttcagaa acggaagttc tctccattcc tattcagaaa acataatctg ctcaaacctg
     4081 ctccaccaaa ggcgctgcta tttatgaaca ataaaaaaac attactataa atccgttcgg
     4141 ttattttttg ggagagaggg taactaccgg aacgatcatc tcttccaaag aaacgccccc
     4201 atgctgaaag gtgtttttga aatagttagc gtaataattg aaattatttg gataagcaaa
     4261 gaagtcggta ttttgggcaa aaatataagt tgtacttaca tttcgggagg gcaggaacgc
     4321 atcgcgggga ttggtcacat caaacacctg ccggcttttg taattcagat tgcgccccag
     4381 tttgtagcgc aggttggtgt tggtctcccg gtcgccaatc acttttacgg ggttgtcaac
     4441 ccggatggtg ccgtggtcgg tggtgataac cacccggata tcatttccgg ccagctcttt
     4501 cagcagctcc agcagcgaag agtggttaaa ccagctcagg gtcagcgacc ggtatgcatt
     4561 ttcattgccg gccagctcgc ggatcatatc catttcggtg cgggcgtgcg agatcatatc
     4621 cacgaagttg aagaccatta ccgacagatc attctccagg atggcgctga gatcttcaac
     4681 caatttcctt ccgcttttca gatttgaaat tttatcgaaa aaaagcttct ctttcctgcc
     4741 cagccgaagc atctgtttga ggagcaactc ttcttcgtgt tcatttttat tcccttcatt
     4801 atcgtcatct tcccacagat tgggatacag cttttcgatt tccgacggca ttaacccggc
     4861 aaacatggca ttgcgcgcat acatggttgc cgttggcagg atgctgtaat agagctcttc
     4921 ttcatcggta cggaaatatt cgctcacaac gctgctcatc acccgccact gatcgaaacg
     4981 cagattatcg atcagcaaca caaaaacctt ccgcccgttg ttgagaaggg gaaaaacctt
     5041 ctggcggaaa atgtcggtgg aaagtagcgg acgttgtccc agcggtttac caaaccagtg
     5101 gaggtaattt tcgcggataa accggcaaaa agcctggtta gcatcatttt tctgcatggc
     5161 aagcacttcg tccatggccg agtcttccga cgaactcagc tccagctccc agtggaccag
     5221 cttccgatag atctctttcc actcttcaaa gctcagccgg tcgttgatcc gcatcccgat
     5281 ctgggcaaac tgcatctgat aggccgaagt ggtttgctgg gttaccagcc gcttctgttc
     5341 tacattcttc ttcagcgaaa gcaggatctg tttgggattt accggtttga tgaggtaatc
     5401 ggtaattttt gaaccgatcg cctcgtccat aatattctcc tcctcgcttt tggtaatcat
     5461 aactaccggc acgttgggag aaatggtttt aatctgactg agagtttcaa ggccgctcag
     5521 tccgggcatg ttttcatcga ggaaaataat atcaaaatgc tcttttttca cccagtcgag
     5581 tgcatcggaa ccgttgttgg cggtttccac ttcatatcct ttttcgcgga gaaaaatgat
     5641 gtgcgggcgc aacaggtcga tctcatcatc ggtccaaagt atcctgattt tcctcataaa
     5701 aaaacacttt acatggtttt caatttaaac aaaaattttg cctcgccggt atgaggtt
//
LOCUS       DAOSDR010000208         5705 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrimonas sp. isolate AMR_MDS_1826
            NODE_5044_length_5705_cov_3.266549, whole genome shotgun sequence.
ACCESSION   DAOSDR010000208 DAOSDR010000000
VERSION     DAOSDR010000208.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863355
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrimonas sp. (wastewater metagenome)
  ORGANISM  Petrimonas sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Dysgonomonadaceae; Petrimonas.
REFERENCE   1  (bases 1 to 5705)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5705)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:10:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,720
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 35
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5705
                     /organism="Petrimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5044_length_5705_cov_3.266549"
                     /isolate="AMR_MDS_1826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2023866"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2409)
                     /locus_tag="PLI30_08800"
     CDS             complement(<1..2409)
                     /locus_tag="PLI30_08800"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HOI79688.1"
                     /translation="MLNKIINYSLNNKLFVLLGAVLLIVGGLFTVQKMDVDVFPDLTA
                     PTVAVMTDAHGMSAEEVERLVTFHIETSVNGATNVRRVRSASMQGYSFVWVEFDWGAD
                     IFKARQVVSEKMVTLGSVLPEGIVPVLAPQSSIMGEILFIGLKSDSTSQMELRTLAEW
                     VVKPAILATGGVSQVTIIGGDYKQYQLLADPQKMDFLGVSMNELAQAGRSFSANSSGG
                     VVRDFGNEYMIRGMARSNDLEELGNTHIKTVNNRPVVVSDVAEVVIGSAVKYGYASEN
                     AEPAVIIAVSKQPNINTLKVTENIEKNLGEIGKSLPRDVVMNTRIFRQADFIESSVRN
                     VGSAMLEGALFVVVILFLFLASFRTTIISIVAIPLSLFGTVIVLHFLGMNINTMTLGG
                     MTIAIGSLVDDAIIDVENVYKRLRQNHRKPREERRPVFDIVLNASIEIRASILNATLI
                     IMVAFIPLFFLTGMEGRMLKPLGVAFIVALFMSLIVAMTVTPLMCKLLLSNEKYLDKN
                     KADSWLTVRLTSLYEKSLEWVLRNKKKVLYPTIGLFLVSVGLFFTMGRSFLPEFNEGA
                     LVITAVTKPGVSLEETNRLGNLMETELLKIPEVASTARRTGRGELDEHSQTSNSSEID
                     VQFRLTKRSNEEFMKEVRNVLAAVPGMAVTVGQPLGHRIDHMLSGTRASIAIKLFGTD
                     LSDLFMLGNQIQHSIKGIDGLVDVSVEQQTETPQLQLRANRGMLSKYGISVEDFNRFV
                     ELAFAGEKLADIYEGQRSFELILRLNENYTESIDRVRSALIDVGSGRKVPLEEIADIV
                     SVGGP"
     gene            complement(2426..3928)
                     /locus_tag="PLI30_08805"
     CDS             complement(2426..3928)
                     /locus_tag="PLI30_08805"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HOI79689.1"
                     /translation="MKLTYLVSALFLAVATLSCNRNNDQDLDHGHVDNLQLISYSDGF
                     EMFAEATPFAVGHESEILAHFTRLENFKPLENGSVTANLVVGDDTVSQTLDSPMRPGI
                     FRFTLKPGEKGEAKLLFNVKISGKISQLTVEGITVFADLNDAMHAAEDAVITSSNGAV
                     FTKEQSWKVDFATEEIRKEPFGQVIRTIGQIQPSPGDERIVVAKTGGVVLFPGDDVVE
                     GRAVGAGETLFTIDGSGMADNNLSVRFSEARNEYSLARAEYDRKAELAKDRIVSQREL
                     RRAETEMENARANFNNLQKNFSAGKQSVNSPIGGFIAAVLVRNGEYAEAGQPVLRVSQ
                     NRNLFVKAELRPKYFEELNRISTVTFRFMENKATYTLEELNGRVVSFGKSADTGNPLL
                     PVLFQVDNRAGFIPGSFIEMFIRTQAGEEVLTVANEAVVEEMGNYFVYVQLTPELFEK
                     RLVKRGRTDGLRTEITTGVSEAERVVSKGAILVKLAQAAGAIDVHSGHVH"
     assembly_gap    3947..4046
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(4172..4618)
                     /locus_tag="PLI30_08810"
     CDS             complement(4172..4618)
                     /locus_tag="PLI30_08810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI79690.1"
                     /translation="MSRAIGISLMFLANMVLLAHAFIPHHHPFSESCSISYHQSDAVH
                     HCRHLDSKANFSENDRNAHRELGLKDCLLDNIYIRFVNDNHTCQPGGHDVDSDPLFSL
                     LYFPADYSNRIEPDSMPLPFLEKPYLASLYTGHIAQSKGLRAPPSC"
     gene            4791..5672
                     /locus_tag="PLI30_08815"
     CDS             4791..5672
                     /locus_tag="PLI30_08815"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008158192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HOI79691.1"
                     /translation="MKNLSRRKFIRTGLAGFAGLTVGGPGLLKLTYTPEITVDRVKLG
                     NSGLNVSRIALGTGSRGGSKASNQTRLGMEKFVEIAHHAYQRGIRFFDMADSYGSHPY
                     VGEAIKTLPREKITLLTKIWTYEDGSDKNIPVRESLDRYREELGTGYIDIVLMHCLMQ
                     GGWSQNRRHYMDGLLKAKQDGIIKAVGVSCHNWDAMAEAVDNPWVDVIMARINPFQSH
                     MDGTPDAVKELLGKARKNGKGVIGMKIFGEGRHVSDGEREQSLKFALTEANIHCMTLG
                     LESIAQVDDAVGRVMAQ"
BASE COUNT         1475 a         1478 c         1274 g         1378 t
ORIGIN      
        1 tgggccgcca actgaaacaa tatcggcaat ctcttccagc ggcactttgc ggccgctgcc
       61 cacatcaatc aacgccgaac ggacccggtc tatactttcg gtatagtttt cgttcagccg
      121 caagatcagc tcaaagctac gctgcccttc gtaaatatcg gctaacttct ccccggcaaa
      181 ggccagttcc acaaaccggt taaagtcttc taccgagata ccgtacttcg agagcattcc
      241 cctgttagcc ctcagttgca gttgcggcgt ctccgtctgt tgctccaccg aaacatccac
      301 caagccgtca atccctttta tcgagtgttg aatctggttg cccagcataa acaaatcgct
      361 caggtcggta ccgaaaagtt ttatggctat gcttgcccgc gttcccgaga gcatatgatc
      421 aatgcggtgt ccgagcggtt gtcccacggt caccgccatt ccggggacag ccgccaacac
      481 gttgcggacc tccttcataa actcctcgtt gctccttttt gtcaatctga actgcacatc
      541 aatctcggag ctgttgctcg tctgcgaatg ttcgtccagt tcaccacgtc cggtacggcg
      601 tgcagtgctg gccacctccg ggatcttcag caactctgtt tccatcaggt ttcccaaccg
      661 attggtttcc tccagtgaaa ctccgggttt ggtcactgcc gttattacca gggctccctc
      721 gttaaattcc ggcaagaaac ttcgccccat ggtaaagaac aagccaaccg aaaccagaaa
      781 caagccaatc gtaggataaa gcaccttctt cttgttccgg agcacccatt cgagtgattt
      841 ttcataaagg cttgtcagcc tgaccgtcag ccagctgtct gctttgtttt tatccaggta
      901 cttttcgttg gagagcaaca gtttgcacat cagcggagtg actgtcatgg cgacaatcaa
      961 cgacataaac agcgcgacga taaatgcaac accaagcggt ttcagcatac gtccttccat
     1021 tccggtaagg aagaacaacg gaataaaggc caccataata atcaatgtcg cattaagaat
     1081 ggatgcgcga atttcaatgg acgcgttcaa cacaatatcg aataccggcc tcctctcttc
     1141 ccggggcttt cgatggtttt gtcttaaccg cttatacacg ttctccacat caataatggc
     1201 atcatccacc agtgaaccga tggcaatagt cattcctccc aatgtcatcg tgttgatgtt
     1261 catccccaga aagtgcagga cgataaccgt tccgaacaat gaaagcggga tggccacgat
     1321 ggagatgatc gtcgtgcgaa aactcgctaa aaaaagaaaa aggatgacaa ccacaaacag
     1381 cgctccttct aacatagcgc tccccacatt tctgacagat gattcaataa aatcggcctg
     1441 acggaatatc cgggtattca taaccacatc ccgaggcaac gactttccaa tctcccccag
     1501 gttcttttca atattttcag ttacttttaa cgtgttgatg ttgggttgtt tggaaacggc
     1561 tattatcaca gcaggttcag cattctcgga agcgtatccg tacttcaccg cgctcccgat
     1621 cactacctct gccacatcag acactacaac gggccggttg ttcacggttt taatgtgcgt
     1681 gttccccagt tcctccaggt cgttggaacg ggccattcct cgaatcatgt attcgtttcc
     1741 aaagtcgcgg acaacacctc ccgatgagtt agcgctaaaa cttctcccgg cctgagccag
     1801 ttcattcatg ctcactccca gaaaatccat cttctgcgga tcggccagga gctggtactg
     1861 cttgtaatcc cctccaataa tcgtaacctg tgagacgcct cccgtcgcca ggattgccgg
     1921 cttcaccacc cactccgcca acgtacgcaa ttccatctgc gaggtgctgt cggactttaa
     1981 tccgataaaa aggatttcgc ccataatgct cgactgtggg gcgagtaccg gaacaatacc
     2041 ttcgggcaat acgctgccca acgtaaccat tttctcgctc accacctggc gcgctttaaa
     2101 aatatcggca ccccagtcaa attcaaccca gacaaaggag tatccctgca tcgatgccga
     2161 gcgtacgcgg cgtacgttgg tagcaccgtt tacggaagtt tcaatgtgaa aagtcaccaa
     2221 gcgctcaacc tcttcggcag acatgccgtg ggcatccgtc ataacagcaa cagtaggggc
     2281 agtcagatca gggaacacat ccacatccat cttctggacc gtgaataaac cgcccacaat
     2341 gagaagaacc gcacccaaca acacaaaaag cttgttattg agtgaatagt ttattatctt
     2401 attcagcata attcaggatc tttaattaat gaacgtggcc ggaatgaaca tcgattgcac
     2461 ccgcagcctg tgccagcttg accaggatag ctcccttgct gacaacacgt tcagcctccg
     2521 aaacgcccgt cgtgatttct gtgcgaagcc cgtcggtccg tcctcttttc accaaacgtt
     2581 tttcaaaaag ttcgggagtc agttgaacat acacaaaata attgcccatc tcctccacta
     2641 ccgcttcatt ggcaacagtc aacacctctt ctcccgcctg cgtccgaata aacatttcga
     2701 taaaactacc gggaataaaa ccggcccggt tgtcaacctg aaacagtacc ggaagaaggg
     2761 gattgcccgt gtcggccgat tttccgaacg aaactacacg gccattcagc tcttccagcg
     2821 tgtaggttgc tttgttttcc ataaagcgga aagtaacggt ggaaatacgg ttgagttctt
     2881 caaaatattt gggccgcagt tccgctttaa caaataagtt cctgttttga gaaaccctta
     2941 aaacaggctg tccggcttct gcatactctc cgttgcgtac caggacagcc gcaataaacc
     3001 cgccgatggg tgaattcacc gattgtttcc cggcagagaa gttcttttgc aggttattga
     3061 aatttgcccg ggcattttcc atttcggttt ctgccctccg caactcgcgt tgcgaaacaa
     3121 tccggtcttt cgccagttca gccttgcggt cgtattctgc cctggccagg ctatactcgt
     3181 tcctagcctc tgagaaacgg acggagaggt tgttgtcggc catgccgctg ccatcgatgg
     3241 taaacaacgt ttctcccgca cccacagctc tcccctccac cacatcatct ccgggaaaga
     3301 gcaccacacc tccagttttt gccacgacaa tacgttcatc gcccggcgac ggctggattt
     3361 gcccgatcgt ccggatgacc tgtccgaaag gctccttccg gatctcttcg gtagcaaaat
     3421 caaccttcca gctttgttct ttggtaaaaa cagcaccgtt gctgcttgta atcaccgcat
     3481 cctctgccgc atgcatagcg tcgttcaggt cggcaaacac ggtgattccc tcaacggtaa
     3541 gctgcgatat tttaccggat attttcacgt tgaacagcag ctttgcttct cctttctccc
     3601 cgggtttcag ggtaaatctg aaaattcccg ggcgcatggg tgaatcgagg gtttggctta
     3661 cggtatcgtc acctacaacc aggtttgccg ttacgcttcc gttttccagc ggtttaaaat
     3721 tttccaaacg agtgaaatga gcaaggattt cgctttcgtg accaacagca aagggagttg
     3781 cctctgcaaa catttcaaag ccgtcactgt aggaaatcag ctgcagatta tccacgtggc
     3841 cgtggtctag gtcctgatcg ttgttccggt tacaagacaa ggttgcaacc gccagaaaca
     3901 acgctgaaac caaataagtt aatttcattg taactgattt taatggnnnn nnnnnnnnnn
     3961 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4021 nnnnnnnnnn nnnnnnnnnn nnnnnnccag aaacaacgct gaaaccaaat aagttaattt
     4081 cattgtaact gattttaatg ggatataaaa agaaatgaat aatcaaccgg aaagagttat
     4141 tccaggaatt gatcgtaaaa aaagtgaaaa atcagcagga aggaggagct cgcaatcctt
     4201 tggattgagc aatatggccg gtatataaag aggctaaata cggtttttcc aggaaaggca
     4261 acggcatact gtccggttca atccggttgg aataatctgc cggaaagtac aacagcgaaa
     4321 acagggggtc actgtctaca tcatgtccgc ctggctgaca agtatggttg tcattgacaa
     4381 accggatgta gatattgtcc agcaggcaat ctttcagacc caactcccgg tgagcattcc
     4441 tgtcgttttc ggagaaattg gctttgctat ccaggtgccg gcaatgatgc accgcatcgc
     4501 tttgatgata agatatactg caggattcag aaaacggatg gtgatgcgga ataaatgcat
     4561 gagccaataa aaccatatta gctaaaaaca tcaaacttat tccaattgct ctgctcatcc
     4621 ttaagtatgt ccacaaaggt aaataaaaaa ttctattcca tccaaacgga acagcctggg
     4681 ctggcggtta aatttcacga taaaaacgcg ttattgcttg tatttatgaa tttattgcgt
     4741 atttttgaga cgtcgaacat tttaaaaaca aaaggaaaac ggacaaagtc atgaaaaatt
     4801 tatcgcgtcg aaaatttatc cgtaccgggc tggcaggctt tgccgggttg accgtgggcg
     4861 gaccgggctt attgaaatta acctatactc ccgaaattac cgttgaccgg gttaaactgg
     4921 gaaactcagg gctgaacgtg tcacgtattg ctttgggtac aggttcccgg ggaggatcaa
     4981 aggcatcaaa ccaaacccgg ttaggtatgg agaaatttgt cgagatagct caccatgcct
     5041 accaaagagg gatccgcttc ttcgatatgg ccgactcgta cggatcgcac ccgtatgtcg
     5101 gcgaagcaat aaagaccctt ccccgtgaaa aaatcaccct gctgacaaag atctggacgt
     5161 acgaagacgg gtcggacaaa aacattccgg tacgggaatc cctggaccgt taccgcgagg
     5221 agctcggcac cgggtatatc gatattgtgc ttatgcattg cctgatgcag gggggttgga
     5281 gtcaaaaccg caggcattat atggatggat tgttgaaagc caaacaagac ggtatcatca
     5341 aagcggtggg agtctcctgc cacaactggg atgccatggc tgaagcagtg gataacccgt
     5401 gggtggatgt catcatggcg cgcatcaatc cgtttcaaag ccacatggac ggtactcccg
     5461 acgcggtaaa agaacttctg ggaaaagccc ggaagaacgg aaaaggggtt ataggcatga
     5521 agattttcgg tgaaggccgt cacgtaagtg acggtgaacg cgaacagtcg ttgaagtttg
     5581 cgcttaccga ggccaacatc cattgcatga ccttagggtt ggaatccatt gcccaggtag
     5641 acgacgctgt aggcagggtc atggcacagt aacacttctt ttacagtaac cgcccccagt
     5701 aaacc
//
LOCUS       DAOSDR010000209         5695 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrimonas sp. isolate AMR_MDS_1826
            NODE_5064_length_5695_cov_4.014184, whole genome shotgun sequence.
ACCESSION   DAOSDR010000209 DAOSDR010000000
VERSION     DAOSDR010000209.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863355
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrimonas sp. (wastewater metagenome)
  ORGANISM  Petrimonas sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Dysgonomonadaceae; Petrimonas.
REFERENCE   1  (bases 1 to 5695)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5695)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:10:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,720
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 35
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5695
                     /organism="Petrimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5064_length_5695_cov_4.014184"
                     /isolate="AMR_MDS_1826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2023866"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1032)
                     /locus_tag="PLI30_08820"
     CDS             complement(<1..1032)
                     /locus_tag="PLI30_08820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019539490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOI79692.1"
                     /translation="MDRRNFLKRSAVTATGLSLTPLMGKSYSSVFGQSAPGNKIKIGL
                     IGCRNQGWANLKTFLDYPGTECISLCDVDDQWLYQRAADVEKITGKKPPQLVKDWRRV
                     IDNKDIDMVIIGTPDHWHCLQLVAACETGKDVYVEKPLANTMEECDLMVRATRKYNRI
                     VQVGQWQRSDPHWDEAAAYVQSGKLGRVRTVKVWAYQTSKWTLPVVPDSAPPAGVDYD
                     MWLGPAPKRTYNQNRFHYNFRFFWDYAGGLMADWGVHLLDYAMKGMNVGLPSYVYGAG
                     GKFGYPDDAMETPDTLMATYKYPDFNIIWDHACGIGNGLFGLREGVAFFGENGTLVLT
                     RQGWEVMPEQ"
     gene            complement(1040..2398)
                     /locus_tag="PLI30_08825"
     CDS             complement(1040..2398)
                     /locus_tag="PLI30_08825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004317107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1080 domain-containing protein"
                     /protein_id="HOI79693.1"
                     /translation="MKRNILVLIATVFIVSASAQEAKWQDLFNGRNLKGWTKLNGTAE
                     YKIQEKAIVGISKSGTPNTFLATNKMYDDFILEFDFKVDDGLNSGVQFRSNSLKEYQN
                     GRVHGYQFEIDPSDRSWTGGVYDEARRGWLYPMTVNPIGQKAFKHNQWNKARIEAIGN
                     SIRTWVNGVPCADLLDEVTKSGFIALQVHSINNKELEGKTVSWRNIRILTQDLDKFRS
                     RENTDGIEQVNTIANTISEREAREGWKLLWDGKTTNGWRGAKLDNFPEKGWSVDNGIL
                     KVQKSGGGESTNGGDIVTTRPYKNFMLKVDFKITEGANSGIKYFVDTNLNKGAGSAIG
                     CEFQILDDKKHPDAKLGVAGNRTLGSLYDLIAAPDNKPFRPGFFNTAMVVVKGNHVEH
                     WLNGVKIIEYERNNQMWDALVNYSKYKDWPDFGNAEEGLLLLQDHGDEVWFQNIKIKE
                     LE"
     gene            complement(2412..3833)
                     /locus_tag="PLI30_08830"
     CDS             complement(2412..3833)
                     /locus_tag="PLI30_08830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004322266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOI79694.1"
                     /translation="MTTRRDFLRNTALASAGIALSGIYTKTNAASYSRIVGANRKVNL
                     AHIGIGNRGWEIINDFDKTKLANVVVLCDVDLGAEHTQKAMAKFPNAKRFKDFREMFD
                     KIGNEFEAVAIATPDFAHFPSVMLAIKEGKHVYVEKPMGRTFNEVELMIKAARKYPKV
                     VTQMGNQGHSEGNYFQFKAWKEAGIIKDVTAITAHMNNARRWHGWDPNIKKFPGAEPI
                     PETMDWDTWLSAQSYHDYNKDFHYGQWRCWYDFGMGALGDWGAHILDTAHEFLDLGLP
                     EEVNPVKLSKHNDYFFPMSSTIEFKFPKRGKMPPVIITWYDGVDNIPSVPEGYGVSEL
                     DPNIPSVGGGKIQPAKLNPGKEIYSKDLIFKGGSHGSTLSIIPDDKAREMERKLPVVP
                     KSPSNHFANFLLACQGEEKTRSPFEIAGPLSQVFCLGVAVQRLNRRIVFDRKTKRVVD
                     DVFADAFLTGAPPRKGWEHFYEI"
     gene            complement(3935..5221)
                     /locus_tag="PLI30_08835"
     CDS             complement(3935..5221)
                     /locus_tag="PLI30_08835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019538277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050043 - lactate racemase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactate racemase domain-containing protein"
                     /protein_id="HOI79695.1"
                     /translation="MIYYQNGSATNNLSREDLEAGLREAFGKLGEKHKVLAIPPDYTR
                     LPSRAGELTEISWEYYGDRLTDILPALGTHTPMTDDQISHMFGKTPANLVRIHDWRND
                     VVTLGRVSAEIVEEVSEYKVHFDWPVQVNRLLVEGNFDLILSIGQVVPHEVVGMANYN
                     KNVFVGTGGFEAINKSHYVGAVYGMERMMGRADTPVRRLFNYASENFAKQLPIVYVLT
                     VVGVNKDGRQQTYGLFVGDDFTVFDVAAKLSLEVNFQMVEKPLKKVVVWLDPTEFKST
                     WLGNKSIYRTRMAIADGGELVVLAPALKEFGEDKEIDRLIRKYGYFGTPETLKAVEDN
                     EELRNNLGAAAHLIHGSSEGRFSITYCPGKGAENLTREEIESVGFRWGDIDETMQKYN
                     FQHLKEGFNILPDGEEVYFISSPALGLWAHRDRFEY"
     gene            complement(5238..>5695)
                     /locus_tag="PLI30_08840"
     CDS             complement(5238..>5695)
                     /locus_tag="PLI30_08840"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019538276.1"
                     /note="catalyzes the formation of D-ribulose 5-phosphate
                     from 6-phospho-D-gluconate; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HOI79696.1"
                     /translation="SYAQGFALMMEAAKEYNWNLNYGGIALMWRGGCIIRSVFLGDIK
                     KAFDNNPKLENLLLDPFFKESIESVQESWRRVCATALENGIPVPALTSALCYFDGFRN
                     DRLPANLLQAQRDYFGAHQYERVDKPRGEFFHTDWTGRGGNTASSTYQV"
BASE COUNT         1366 a         1511 c         1190 g         1628 t
ORIGIN      
        1 ctgttcgggc atcacctccc atccttgccg ggttagaaca agcgtaccgt tctctccgaa
       61 gaaggcgacc ccttcgcgaa gcccgaacaa tccattacca atgccacagg cgtgatccca
      121 gataatgttg aaatcagggt atttataggt cgccataagt gtatcgggcg tctccatagc
      181 atcatcggga taaccgaatt tccctccggc accgtataca tacgatggca atccgacatt
      241 cattcccttc atggcatagt ccagcaaatg tactccccaa tcagccatca atccgccggc
      301 gtaatcccag aaaaagcgaa aattataatg gaaccggttc tgattatacg ttcgcttcgg
      361 tgcaggaccc agccacatgt catagtctac tccagcggga ggtgccgaat caggcaccac
      421 cggcaacgtc catttgctgg tttggtacgc ccagacctta acggttctca cacgtcccaa
      481 cttaccactc tgcacgtagg cagccgcctc atcccagtgg ggatcgctcc gctgccattg
      541 tcccacctgt acaatccggt tatatttccg ggtagcacgc accatcaggt cacactcctc
      601 catggtattg gcaagcggct tttccacgta tacgtctttt ccggtttcgc aggcggccac
      661 caactgaaga cagtgccagt gatccggcgt accgataatc accatgtcta tatctttgtt
      721 gtcaatcacc cggcgccaat ccttaaccag ttgaggtggt ttttttccgg ttattttttc
      781 cacgtcggca gctcgttgat agagccactg atcgtctacg tcacacaagg agatgcattc
      841 ggtcccgggg tagtcgagga aagttttcaa gttggcccac ccctggttac ggcagccaat
      901 gagtccgatt ttgattttat tcccgggcgc cgattgtccg aaaacggatg agtaggattt
      961 ccccattaat ggagtcaggc ttaggccagt agccgtaacg gctgatctct tgagaaaatt
     1021 tcttcgatcc atgatattgt tattcaagtt ctttaatctt tatgttctga aaccatactt
     1081 catcgccgtg atcctgcaat aagagcaacc cctcttctgc atttccgaaa tcgggccagt
     1141 ctttatactt gctgtaattt accaacgcat cccacatctg gttgttgcgt tcatattcaa
     1201 taattttcac tccgttcaac cagtgttcta cgtgatttcc cttcacaacc accatagccg
     1261 tattaaagaa gccgggcctg aaaggtttgt tatcgggggc tgcaatcaag tcgtaaagtg
     1321 aaccaagtgt cctgtttcca gcaacaccta atttggcatc gggatgtttt ttgtcatcca
     1381 atatttgaaa ttcgcagccg atggctgaac ctgcgccttt gttcaggttt gtgtccacaa
     1441 aatacttgat gccactgttg gcaccttcgg taatcttaaa gtccactttc aacataaaat
     1501 ttttatacgg gcgggtggta acaatatcgc caccgttcgt agattctcct ccaccgcttt
     1561 tttgaacttt gaggattccg ttatcgactg accagccttt ttcgggaaaa ttatcgagct
     1621 ttgctcctcg ccatccgttg gtagtctttc cgtcccacaa cagtttccat ccttcacgtg
     1681 cttctcgctc ggaaatggta ttggcgatgg tatttacttg ttctattccg tctgtgtttt
     1741 cacgcgacct gaacttatcg aggtcctgag tcaggatacg gatgtttctc caggagaccg
     1801 ttttgccttc gagttctttg ttatttattg agtggacctg taaggcaata aaacccgatt
     1861 ttgtaacttc atccaataaa tcggcacagg ggacaccgtt tacccacgtg cgtatggagt
     1921 taccgattgc ctctatgcgt gctttgttcc actgattatg tttaaaggct ttctgtccaa
     1981 tgggattgac agtcatagga taaagccatc ctctgcgtgc ttcatcgtaa acgcctcccg
     2041 tccacgatct gtcggaaggg tcaatctcga actgataccc gtgcacgcgt ccgttttgat
     2101 actctttcaa gctattgctt ctaaactgaa ctccggagtt cagtccgtcg tccactttaa
     2161 agtcaaattc caggataaag tcgtcataca tcttattggt agccaagaat gtattaggcg
     2221 ttccgctttt tgaaatcccg acaatggcct tctcctgaat tttatattcg gctgttccgt
     2281 ttagtttggt ccaccctttc aggttccttc cgttgaagag atcctgccat ttggcttcct
     2341 gagccgaagc agaaacaata aaaaccgttg caattaatac aagaatattc cttttcatag
     2401 attactattt tttatatttc atagaaatgt tcccatcctt ttcgtggagg tgcccctgtc
     2461 agaaaagcat ccgcaaaaac atcgtcaaca acccgttttg tttttcggtc aaagacaatt
     2521 ctccgattca gtcgctgcac tgctaccccc agacagaaca cctggctcag cgggccggca
     2581 atctcgaagg gcgagcgtgt tttctcttct ccctgacagg ccaataggaa attggcgaaa
     2641 tggttcgacg ggcttttcgg aacaacggga agcttcctct ccatttcccg ggctttgtca
     2701 tccggaataa tgcttaacgt actcccgtgc gaaccaccct taaaaatcag gtctttgctg
     2761 taaatttctt tccccggatt cagcttggcc ggttgaattt ttccgcctcc aacactggga
     2821 atattcggat caagttccga aacaccataa ccttccggaa cggaaggaat gttatccaca
     2881 ccgtcgtacc acgttatgat aacgggcggc attttcccgc gctttggaaa tttaaattcg
     2941 atcgtgctcg acatcgggaa gaagtaatcg ttgtgcttgg agagttttac cggattcacc
     3001 tcttccggca aacccagatc aagaaactcg tgggccgtat cgaggatatg cgctccccag
     3061 tcgcccaaag cccccattcc gaaatcgtac cagcaacgcc attgtccgta gtgaaagtcc
     3121 ttgttgtaat cgtgatacga ctgggccgac aaccaggtgt cccaatccat tgtttccggg
     3181 atgggctcgg caccagggaa cttcttgatg ttcggatccc atccgtgcca gcggcgggcg
     3241 ttgttcatgt gcgctgtaat ggcggtaaca tcttttatga tgccggcttc tttccaggct
     3301 ttgaactgga aataattacc ttcagaatgt ccctggttac ccatctgcgt tacaactttt
     3361 ggatacttcc gggctgcttt tatcatcagt tcaacttcgt taaatgtgcg gcccataggt
     3421 ttttccacat agacatgctt tccttcttta atggctagca tcactgatgg gaaatgagca
     3481 aaatcgggag tggcgatggc taccgcctca aactcgtttc cgattttatc gaacatctct
     3541 ctaaaatcct tgaaacgttt tgcgtttgga aatttagcca tggctttttg ggtgtgctcg
     3601 gctcctaaat ccacatcaca gaggacaaca acgtttgcca attttgtttt atcaaaatcg
     3661 ttgataatct cccaacctct gtttcctatc ccgatgtgtg ccagatttac ttttcgatta
     3721 gcccctacaa tacggctgta gctagctgca ttggtcttgg tatagatacc gcttaacgca
     3781 attcccgcag aggctaaagc tgtatttctc aagaaatctc ttcttgtcgt cataatatat
     3841 attctaacgt atttgttttt gtagaaaaac aaaaatataa aaaaacgcct tttaaagcaa
     3901 aaaatgcctg gatctctata ttcttgaatg acttttaata ctcaaaccgg tctctgtgtg
     3961 cccataaccc cagagcgggg cttgaaataa aatacacctc ttccccgtcc ggcaggatgt
     4021 taaatccttc tttcaggtgt tggaaattat atttttgcat cgtttcatcg atatctcccc
     4081 accgaaagcc cacactttca atctcttccc gtgtgaggtt ttctgctcct ttacccggac
     4141 aataggtgat ggaaaacctg ccttccgacg acccgtgaat aagatgtgcc gcggcaccca
     4201 gattattcct caactcttcg ttatcctcaa ctgcttttaa tgtttcagga gttccaaaat
     4261 aaccgtattt ccggatcaac cggtctatct ccttatcctc tccgaattcc tttaatgccg
     4321 gcgccaacac aacaagctcc cctccgtcgg caatagccat tcgtgttctg taaatggatt
     4381 tgtttcccag ccaggtgctt ttgaactccg tggggtctaa ccacactacg accttcttca
     4441 acggtttttc aaccatttgg aaattaacct cgagagagag cttggcagct acgtcaaaaa
     4501 cggtgaaatc atcgcccaca aacagaccgt aagtctgttg cctcccgtcc ttatttaccc
     4561 ccactaccgt aagcacatac acaataggga gctgtttagc aaaattttcc gaagcataat
     4621 tgaatagcct gcgaaccggg gtatcggcac gtcccatcat tcgttccatg ccatagacag
     4681 ctcccacgta gtggctcttg ttgatcgctt cgaatccacc ggttcccaca aaaacgttct
     4741 tgttatagtt tgccatcccc accacttcgt gtggaacaac ctgtccgata gaaaggatga
     4801 ggtcgaaatt gccctcaacc aacaacctat ttacttgaac cggccagtcg aagtgtactt
     4861 tatattcgga cacctcttcc acaatttctg ccgagaccct accaagcgtt accacatcgt
     4921 ttcgccagtc gtgaattcgt accaaattag ccggagtttt tccgaacatg tgtgagatct
     4981 gatcatcggt catgggtgta tgcgtcccca acgccggaag aatatcggtt agcctgtcac
     5041 cataatattc ccagcttatt tctgtcagtt cacccgcacg gctgggcagg cgagtataat
     5101 cgggcgggat agccagcact ttatgctttt cacccaattt accgaaagcc tcgcgaagac
     5161 ccgcctccag gtcttcgcgc gacaggttgt ttgtggcaga gccgttttga taataaatca
     5221 tctcttcaga ttgaatacta aacttggtat gtggacgagg cggtatttcc tccgcgacct
     5281 gtccagtcgg tatggaagaa ctcaccgcgc ggtttatcta ccctctcata ctgatgagct
     5341 ccaaaataat cacgctgcgc ctgcagcagg ttagccggta agcggtcgtt acggaacccg
     5401 tcgaaatagc acaaagccga tgtcagtgca ggaacaggaa ttccgttttc gagtgcagta
     5461 gcgcacaccc tgcgccaact ttcctgaacc gattcgatac tttctttgaa gaacggatcg
     5521 agcaacagat tttccagctt gggattattg tcgaatgctt ttttgatgtc acccagaaat
     5581 accgaacgga tgatgcatcc tccacgccac atcagcgcta ttcctccgta attgaggttc
     5641 cagttatatt ctttggcagc ctccatcata agggcgaacc cctgtgcata cgaaa
//
LOCUS       DAOSDR010000210         5686 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrimonas sp. isolate AMR_MDS_1826
            NODE_5074_length_5686_cov_3.995205, whole genome shotgun sequence.
ACCESSION   DAOSDR010000210 DAOSDR010000000
VERSION     DAOSDR010000210.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863355
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrimonas sp. (wastewater metagenome)
  ORGANISM  Petrimonas sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Dysgonomonadaceae; Petrimonas.
REFERENCE   1  (bases 1 to 5686)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5686)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:10:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,720
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 35
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5686
                     /organism="Petrimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5074_length_5686_cov_3.995205"
                     /isolate="AMR_MDS_1826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2023866"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..3386)
                     /locus_tag="PLI30_08845"
     CDS             complement(<1..3386)
                     /locus_tag="PLI30_08845"
                     /inference="COORDINATES: protein motif:HMM:NF013957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004866 - endopeptidase inhibitor activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MG2 domain-containing protein"
                     /protein_id="HOI79697.1"
                     /translation="MNYDQAWKKVAELEQKSLPRSASEQVNQILRKAIAEKNSPQTIK
                     ALIHQGKYNLAVDAEQDTLIFRHLTDMLGKSTDPVERSVLHSMLGELYLQYYQKDRWI
                     INERTAISGFVPTDMKEWSKNNFYDKVVEHLNASIDQRAQLEKAGVQPFEPVVTLGKD
                     SRRLYPTMYDFLALRAIELFSQVGEDTDLSRSLAKKSIALSSLFAPAGEFVKLNFDPQ
                     PEEYNLWALETYKKLFVSLFKRDLNISVVLTELDKTGYLAKLHNAHQQYALPLLQSML
                     KKWGNDPISVEIVDGMANIYTTQIGRLTQEDNLKRMEKTKELYDLLHKTIQSFPNNER
                     ASILENRLLQLTQPYFLVKGNNTFDAEVEKKLVVEYKNLRKLHAKLYRLDSPLSAVYS
                     SNRGRQQTDEKKTLIREIWIPLSSRQPYERDQTELELNITEFGSYKLEFESDPVAEKE
                     GNNSFYFSVSGLAVFSRLSAKDTYEFFVVDRVNGQPVKNARINIYKLPGSWYNSALTL
                     ETTIPVNGVGLAVYNKNISNNDVFYQAVAENDNGSPLHSFPWSYYPQEDRRTQTAETV
                     NLFTDRSLYRPGQTVFFKAVATTTKDDSSQVTANKSLEFVLRDANDQEVSRQVLKTNE
                     FGSVSGEFVLPQGSLTGYFRITTHNGNAGFRVEEYKRPAFEVTFDKIETTYKFGEEIT
                     LTGKAESYSGVKLQNAHVSYRITRQQSWWWRWGGTPEHFTEGSVKTDENGKFTVVFIP
                     QKADGSSSSRSVYTFDVEATVTDVNGETQVGTYSVTVGDISMMLNMEMPEKLEKSSDE
                     KIVVSARNLDGNDIKASGTYRVFSLLENDSTHRQLLQGNFETGEQTELKSKLKALPSG
                     KYRLRLLSKDDRGNDITAGHDFILFDYSDKRPPVKTNEWFVVKNRVFGPGKDAEIVLG
                     ATDDLNVLYELWREGVLLERKWVKINNGNRLFSIPFKAGYKTGVVLMLTYVKDEKFYH
                     HSTELISEKEKTGLEVKLDIFRDKIRPGAQEEWRLTIKDAKGNPATAEVLASMYDFSL
                     DRIYSTPKWVLSLPQFYMYRTAAPLEKDRSFNPQQVNGYFAVPFKNVESLFFDRLNWY
                     GFSLYRYGRMMVRGVLQEKVAGVQADYYAAPMAKGETLQMA"
     gene            complement(3674..>5686)
                     /locus_tag="PLI30_08850"
     CDS             complement(3674..>5686)
                     /locus_tag="PLI30_08850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004310067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HOI79698.1"
                     /translation="DNLGFGDFIIIAPKSKEEIFRIKNLKELQLNIRQIPDDSLYYHL
                     SRNHFSRFFYTRAMFPVAEMLKKIDVSAYAKMDDARELIYQAIVEYRRMKNTGVVAVF
                     EKDRFDKYSNFARIGDGSLGGKGRGLAFIGHIVKTHLELNSYENFPVTTPKTVVLCTD
                     IFDEFMEANRLYEIALSPRPDEDILKHFLAAKLPKRLVSDFLVFFDAISTPIAIRSSS
                     MLEDSQYQPFAGIYSTYMIPYVNDKYEMVRLLSNAVKAVYASVFYKDSKAYMTATQNL
                     IDQEKMAIVLQEVVGGEYGNLFYPAVSGVARSINYYPIGNEKTEDGIVNMAMGLGKYI
                     MDGYQGLRFSPLHPKNILQLSSLDTALRDTQTQFYALDLESMSKDFTIDDSFNLQKIR
                     IQQAGTNSPLRYVCSTYDPDDQIIRDGFYEGGRKVVSFANMLKHDTLPLAETLDKVLK
                     VGQQEMGRPVEIEFAVNIKSQQEAEFFVLQIRPIVDNKEVVNEDLENIDKSETVLYSR
                     NALGNGISTDVSDVVYVKTKHFSAANNPAIAREIEKVNIRFSEADKNYVLVGPGRWGS
                     SDPWLGIPVKWAHISQARVIVESGLENYRIEPSQGTHFFQNLTSFGVGYFTINSFSQQ
                     DGFFDEDFLDSQPAVYETDFIRHVCFDQPLPIKISGKKKIGVVLKP"
BASE COUNT         1486 a         1384 c         1179 g         1637 t
ORIGIN      
        1 gccatttgaa gagtttctcc ttttgccatc ggcgcagcat aataatctgc ctgaactcct
       61 gccacttttt cttgtaatac acctctgacc atcatcctcc cgtatcgata caacgaaaag
      121 ccgtaccagt ttagccgatc aaaaaacaac gattccacgt tcttaaaagg tactgcaaaa
      181 tatccattca cctgctgtgg attgaaagat cggtcttttt ccaaaggagc agccgtcctg
      241 tacatataaa actgaggcag gctcaacacc catttcggtg tggaataaat tcgatcgagc
      301 gaaaaatcgt acatcgaggc caatacttct gctgtagcgg gattcccctt ggcatctttt
      361 atcgtcaacc gccattcttc ctgggcacca ggccgtattt tatcacgaaa aatgtccagt
      421 tttacctcca gtccggtttt ttctttctca gaaataagtt ctgtggaatg atggtagaat
      481 ttctcatctt tcacatatgt cagcataagt acgacaccgg ttttgtagcc cgctttaaag
      541 ggaatggaaa acaagcggtt cccgttgttt atcttcaccc atttcctttc cagcagcact
      601 ccctcccgcc agagttcata caacacgttc aggtcatctg tagcccctag aacaatctcc
      661 gcgtctttgc cgggcccgaa taccctgttc ttcacaacaa accactcgtt ggtttttacg
      721 ggagggcgtt tatcggaata gtcgaacaaa ataaaatcgt gtccagcagt aatgtcgtta
      781 ccacgatcgt ctttcgatag cagccgaagg cggtattttc ccgaaggtaa cgctttgagc
      841 ttcgatttca gttccgtttg ttccccggtc tcaaaattgc cctgcaacag ctgcctatgg
      901 gtagagtcgt tttcaagtaa ggagaacacc cgataagtcc ccgatgcttt tatgtcgttc
      961 ccgtctaaat ttctggcgga aacaacaatc ttttcgtcag atgatttttc gagtttctcg
     1021 ggcatctcca tgttcagcat catcgaaata tcgcccaccg taacggaata agttccaacc
     1081 tgtgtttcac cgtttacatc ggtaaccgtc gcttccacat caaacgtata gacggagcgt
     1141 gacgaagaac tcccgtcggc tttctgtgga atgaaaacaa cggtaaactt tccgttttca
     1201 tccgttttca cactcccttc ggtgaaatgt tccggcgtcc cgccccaccg ccaccaccag
     1261 gattgttggc gggtaatgcg gtaactcaca tgagcgtttt gcagcttcac accggaatag
     1321 ctttcggctt ttccagtcaa cgtaatttct tcgccaaatt tataggttgt ctcaatcttg
     1381 tcgaacgtaa cctcaaacgc gggacgtttg tactcttcca ccctaaatcc ggcattcccg
     1441 ttgtgggtgg taatcctaaa atatccggtc aatgatccct gcggcaacac aaactcaccc
     1501 gaaacggaac cgaactcatt ggtttttaaa acctggcgcg agacttcctg atcattggca
     1561 tcgcgcaaaa caaattcaag ggatttgttg gcggtaacct gcgagctatc gtcctttgtt
     1621 gtggtggcaa ccgctttgaa aaacacagtt tggccgggac gatacagact tcggtcggta
     1681 aataaattta ccgtttctgc ggtttgtgtc cggcgatctt cctgcggata ataactccag
     1741 ggaaatgaat gcaacgggga tccattgtcg ttttccgcaa cagcctggta aaaaacgtcg
     1801 ttattggaga tgtttttgtt ataaactgcc aatccgactc cgtttacggg aatagtcgtt
     1861 tcgagggtaa gcgctgaatt gtaccagctt ccgggaagct tgtagatatt tatccgggca
     1921 ttttttaccg gttgaccgtt tacccggtct accacaaaaa actcataggt atctttcgcc
     1981 gacaaacggc tgaaaacagc taagcctgaa actgaaaaat aaaaactgtt gttgccttct
     2041 ttttccgcta caggatcaga ctcaaattcg agtttgtagg aaccgaactc agttatgttc
     2101 agttcaagtt cggtttgatc ccgctcatac ggctgcctgg aggaaagagg aatccatatc
     2161 tcacggatca acgttttctt ctcgtctgtt tgctgacgac cgcggttgct gctgtaaacg
     2221 gcagaaaggg gtgaatcgag gcgatagagc ttggcatgta gcttcctgag gtttttgtat
     2281 tccaccacaa gctttttttc tacttccgca tcaaaagtat tgtttccctt taccagaaaa
     2341 tagggttgcg taagctgtaa caacctgttt tccagaatag aggcacgttc attattggga
     2401 aacgattgaa tggtcttgtg caacaaatca tacaattcct ttgttttttc cattctttta
     2461 aggttgtcct cttgcgtgag cctccctatt tgtgtcgtat aaatatttgc catcccgtcc
     2521 acgatttcca cgcttatcgg gtcatttccc catttcttca gcattgattg caacaaagga
     2581 agagcatact gttgatgagc attgtgcagc ttggcaaggt agccggtttt gtcgagttcc
     2641 gtaagtacta cagaaatatt taaatctctt ttgaataagg aaacgaacag ttttttatag
     2701 gtttccaacg cccagaggtt atattcttcg ggctgcggat caaaattcag tttaacaaat
     2761 tcccctgccg gggcaaataa cgacgacaac gctatgcttt tcttcgccag cgaacggctc
     2821 aggtccgtat cttcacctac ctgactgaaa agctcaatag cacgaagcgc taaaaagtcg
     2881 tacatcgtgg gataaagacg acgcgaatcc ttaccgaggg taacaacggg ttcaaaaggc
     2941 tgaacacctg ctttttcgag ctgcgcgcgt tgatcgatgg acgcgtttaa atgctccacc
     3001 actttatcgt agaaattgtt ctttgaccac tctttcatat ctgttggaac aaatccactg
     3061 atcgctgtac gttcgttaat aatccaacgg tctttttggt aatactgcag gtagagttcg
     3121 ccgagcatgg aatgcaacac cgatctttct accgggtccg tagacttacc caacatatca
     3181 gtcaggtggc ggaaaatcaa ggtatcctgt tctgcatcta ccgccaaatt gtatttaccc
     3241 tggtgaatga gggctttgat ggtttgaggg ctgttctttt cggcaatggc ttttcgcagg
     3301 atctgattca cctgttccga tgctgatctg ggaagggatt tctgttccag ttcggccacc
     3361 tttttccagg cctgatcgta attcatagtt tgttggtttt gtgcttgcag cggaaataaa
     3421 aaaatcactg ccgcagcaat cgataaaagt tttttcatac gactaaaatt attaattcaa
     3481 gtttcgcata ttgttcattc agctaaatct tctactgatg atgttttgcg aaccatgttt
     3541 gccctcgttc actaaaagag caaaacacga taaagttcaa ttatatagat gtaaaaacgt
     3601 aaaaaagtga acacaaaact gtgttatttt ttgttaaatc atatcttcct acacttctac
     3661 ttcaggtaaa gaatcacggt ttcaggacta ctcctatttt tttctttcca ctgattttta
     3721 taggcaatgg ttggtcgaaa cagacatggc gtataaaatc cgtctcatag acagcaggtt
     3781 gcgaatcaag aaaatcctcg tcaaaaaaac cgtcttgctg ggaaaaggag ttaatcgtga
     3841 aatagcccac gccaaacgag gtgaggtttt gaaagaaatg agtaccctga ctcggctcga
     3901 tccggtaatt ctccaatccc gactccacaa ttacgcgtgc ctggcttatg tgggcccatt
     3961 tgaccggaat ccccagccac gggtctgacg atccccaccg cccgggacct accagcacgt
     4021 agttcttgtc cgcctcgctg aaacggatat ttactttttc aatctcccgg gctattgcag
     4081 gattattggc tgctgaaaaa tgcttcgttt tcacataaac cacatcggaa acatcggtag
     4141 atatcccgtt tccaagagca tttcgtgaat aaagcactgt ctcggatttg tcaatatttt
     4201 ccaggtcttc gtttacgact tccttattgt ctacaatcgg acggatttgc aatacgaaaa
     4261 actcggcttc ctgctgactc tttatattca cagcaaactc tatttctacc ggccgtccca
     4321 tctcctgctg ccctactttc aacactttgt ccaatgtctc tgcgaggggc agtgtatcgt
     4381 gcttcaacat attggcgaac gaaacgactt ttcttccccc ttcataaaat ccgtcacgga
     4441 taatttgatc atcaggatca tacgttgaac agacataccg taacgggctg tttgtccctg
     4501 cttgctgtat gcgtattttt tgcagattaa aactgtcatc gatcgtgaaa tcttttgaca
     4561 tcgactccag gtcgagtgca taaaactgcg tttgtgtatc tctcaaagca gtgtccagtg
     4621 aacttaactg caatatattt ttaggatgca acggggaaaa acgtagtccc tggtatccgt
     4681 ccataatgta ttttcccaag cccatggcca tgttcacaat accgtcttcc gtcttttcgt
     4741 tgcctatggg ataatagttg atggaacggg caactcccga aactgccgga taaaacaaat
     4801 tcccatactc accacctact acttcctgca acacaatcgc catcttctcc tgatcgataa
     4861 ggttctgggt ggccgtcata taagccttac tgtctttata aaacaccgaa gcgtataccg
     4921 ccttgacagc attactcaac aggcggacca tttcgtattt atcgtttacg taaggaatca
     4981 tatacgttga atatatgcca gcaaaaggct gatactgtga atcttcaagc atactcgaag
     5041 aacgaatggc aatgggtgtg gagatggcat caaaaaaaac cagaaaatcg ctgaccagac
     5101 gcttggggag ttttgccgcc aggaaatgct tgaggatatc ctcatcggga cgaggagaaa
     5161 gcgcaatctc gtaaagcctg ttggcttcca taaattcatc gaagatatcg gtacacaata
     5221 ccactgtctt gggtgtcgtt accgggaaat tctcataact gttcagttcc agatgtgtct
     5281 tcacgatatg gccgatgaaa gccagcccgc gacctttccc ccccaatgaa ccgtcaccga
     5341 tacgggcaaa gttggagtat ttatcgaacc tgtccttttc gaataccgca accacacccg
     5401 tgtttttcat ccgcctgtac tctacgattg cctgatagat aagctcgcgc gcatcgtcca
     5461 tcttggcata agccgacaca tcaatcttct tcagcatttc cgccacaggg aacatggcac
     5521 gtgtatagaa aaaccgcgag aaatggttcc tcgaaaggtg ataatacaaa gaatcatcgg
     5581 gaatctgacg aatattcaac tgcagttcct tcaggttttt gatccggaaa atctcctcct
     5641 tggatttagg agcaataata ataaaatcac cgaatcccag gttatc
//
LOCUS       DAOSDR010000211         5671 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrimonas sp. isolate AMR_MDS_1826
            NODE_5091_length_5671_cov_3.942664, whole genome shotgun sequence.
ACCESSION   DAOSDR010000211 DAOSDR010000000
VERSION     DAOSDR010000211.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863355
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrimonas sp. (wastewater metagenome)
  ORGANISM  Petrimonas sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Dysgonomonadaceae; Petrimonas.
REFERENCE   1  (bases 1 to 5671)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5671)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:10:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,720
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 35
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5671
                     /organism="Petrimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5091_length_5671_cov_3.942664"
                     /isolate="AMR_MDS_1826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2023866"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..243)
                     /locus_tag="PLI30_08855"
     CDS             complement(<1..243)
                     /locus_tag="PLI30_08855"
                     /inference="COORDINATES: protein motif:HMM:NF025092.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HOI79699.1"
                     /translation="MRKFLILFFLPVLSLSVLAQYKEVSGVVIDAQYNTPVPYAAIFV
                     EHTKIGVVASNVGEFSLQVPDSLKNNRLVSVGEGYSL"
     gene            complement(258..1937)
                     /gene="ettA"
                     /locus_tag="PLI30_08860"
     CDS             complement(258..1937)
                     /gene="ettA"
                     /locus_tag="PLI30_08860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019541135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0045900 - negative regulation of
                     translational elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HOI79700.1"
                     /translation="MADDKKIIFSMVGVSKTFPPHKQVLKDIYLSFFYGAKIGIIGLN
                     GSGKSTLLKIIAGIEKEYQGEVVSDKSFSVGYLEQDPKLDEDKSVIEIVREGVQPVMD
                     LLAEYEEINLKFGLPEYYEDEEKMNKLFDRQAELQDKLDAVDAWNIDNRLERAMDALR
                     CPPEEQPVRELSGGERRRVALCRLLLQEPDVLLLDEPTNHLDAESIDWLEQHLQQYKG
                     TVICITHDRYFLDHVAGWILELDRGEGIPWKGNYTSWLEQKTKRMEQEEKQVSKRRKT
                     LERELEWINLAPKARQAKGKARLNSYEQLLNQDQKEREEKLEIFIPNGPRLGNKVIEA
                     KNVKKAFGDKLLFDNLNFMLPPNGIVGVIGPNGAGKTTLFRLIQGMETPDNGSFEVGE
                     TVVTAYVDQAHEAIDPGKTVYQVVSGGLEFVRVGGKEVNSRAYLSRFNFSGADQEKLC
                     GVLSGGERNRLHLALTLKAGANVLLLDEPTNDIDVNTLRALEEGLENFAGCAVVISHD
                     RWFLDRICTHILAFEGNSEVFYFEGSYSEYEENKKMRLGNVEPKRVRYRKL"
     gene            2175..3071
                     /locus_tag="PLI30_08865"
     CDS             2175..3071
                     /locus_tag="PLI30_08865"
                     /inference="COORDINATES: protein motif:HMM:NF015431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HOI79701.1"
                     /translation="MERMDYRDTVFISVVENLSFSKAADELNISQPAVTRHIKELEER
                     YHTNLFERKGNKIYLTRAGEKVYHTFKLIDRQYRELDFEVGELQNTVAGEFRIGASST
                     IAQYVIPKVIASFHKRYPTIKIHLMNGNSFEMEKQLMENSVDIALVENTSSQSDIRYK
                     KFLDDELIVVTGSGSVYAKRETITQDELSRLPMVLREQGSGTLEVIKEAFSRHGIDSE
                     HFHTPIHLGSTESIKNFLLDFDGVAIVSEKAVSGELQLKTLVKLKVSGFSIPRQFRIA
                     YKLGHKSRQVELFENFLLNYNI"
     gene            3145..4056
                     /locus_tag="PLI30_08870"
     CDS             3145..4056
                     /locus_tag="PLI30_08870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006842005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative sulfate exporter family transporter"
                     /protein_id="HOI79702.1"
                     /translation="MNNKSWLYWVVICFCFLPFVSPAVALCIGIVFSFLGIRHDSIHR
                     YTSRVLQVSIVLLGFGMGLEEVITASKSGFVQTLISVSLVMTGGIVLGRLLRVEKNTT
                     LLIAAGTAICGGSAIAAVAPIIKSENYQNSFALIVVFVLNAIALLLFPFIGQRLGLSQ
                     EVFGNWAAIAIHDTSSVVGAGAIYGEKALQVATTVKLIRALWIIPLSIVLAFFSKNGD
                     KRSIKFPWFILLFVAAILFANIFPAFESSYAHFSWLGRRAMVVALFLIGSNITLHQIK
                     QSGTRCFLLGVTLWVVTVAFSLFLLTR"
     gene            4252..5256
                     /gene="gap"
                     /locus_tag="PLI30_08875"
     CDS             4252..5256
                     /gene="gap"
                     /locus_tag="PLI30_08875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009433587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0019682 - glyceraldehyde-3-phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HOI79703.1"
                     /translation="MIKVGINGFGRIGRLVFRAAQTRDDIQVVGINDLLDVNYIAYML
                     KYDTIHGQFTGTIDVKDGHLVVNGKTIRVTAEKNPADLKWGEVGADYVVESTGLFLSK
                     DKAQGHIDAGAKYVVMSAPSKDDTRMYVCGVNENTYTKGEQFVSNASCTTNCLAPITK
                     VLNDKFGVLEGLMTTVHSTTATQKTVDGPSAKDWRGGRAASGNIIPSSTGAAKAVGKV
                     IPELNGKLTGMSMRVPTLNVSVVDLTVRLAKETSYAEICAAMKEASEGELKGILGYTS
                     DDVVSSDFIGDARTSIFDEKAGIQLSPTFVKVISWYDNEWGYSNKVLDLVAHMAKVNG
                     "
     gene            complement(5386..5553)
                     /locus_tag="PLI30_08880"
     CDS             complement(5386..5553)
                     /locus_tag="PLI30_08880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010663521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HOI79704.1"
                     /translation="MAYVINEDCIACGTCIDECPVEAISEGDIYVIDPDVCTDCGTCA
                     EVCPTEAIHPA"
BASE COUNT         1497 a         1368 c         1292 g         1514 t
ORIGIN      
        1 caggctatac ccttctccca cactcaccag gcggttattc ttcaatgaat cgggtacctg
       61 aagtgagaat tcacccacat tactggccac tactccgatc ttggtatgct ccacaaagat
      121 cgcggcgtag gggaccggcg tgttgtactg cgcatcgata actaccccac tgacctcttt
      181 gtattgagca agtactgaca ggcttaatac cggcaagaaa aacagaatca gaaacttcct
      241 cattcttaat tttctgttta cagcttccgg tagcgtaccc ttttcggctc tacgttgcct
      301 aggcgcattt tcttgttctc ctcatactcg ctataggaac cctcgaaata aaatacttct
      361 gaatttcctt caaacgcaag gatatgggta cagatacggt ccaggaacca acggtcgtgc
      421 gagattacca cggcgcatcc ggcaaagttt tccagccctt cttccagtgc acggagggta
      481 ttcacgtcga tatcgttggt cggctcgtcg agcaacagta cgtttgcccc ggctttgagt
      541 gtcaatgcca ggtgtagacg gtttcgttca ccgccggaaa gaacaccgca cagtttttcc
      601 tggtccgctc ccgagaaatt gaatcgcgac aagtatgccc gggagttcac ctctttgccc
      661 cccacgcgta caaactccaa tccaccggac accacctgat agacggtctt accgggatcg
      721 atggcttcgt gagcctgatc cacataagcc gtaacaacgg tctctcccac ctcgaacgag
      781 ccattgtccg gggtctccat tccctgtatc aatcggaaaa gggtggtttt tccagcaccg
      841 ttggggccga taacgccaac gatcccgttg ggtggaagca tgaagttaag gttgtcgaac
      901 agcagcttgt caccaaaggc tttcttcacg tttttagctt caatcacctt gtttcctaac
      961 cgggggccgt tgggaataaa aatctccagt ttctcctcac gctctttctg atcctggttc
     1021 aggagttgct cgtacgagtt cagacgtgct tttcctttgg cctggcgcgc cttgggcgcc
     1081 aggttgatcc attccagctc ccgttccagc gttttgcgac gcttgctcac ctgcttctct
     1141 tcctgttcca tccgtttggt tttctgctcc agccacgagg tgtagttccc tttccagggg
     1201 attccctcac cgcggtcgag ttcaagaatc cagcccgcca catggtcgag gaagtaacgg
     1261 tcgtgcgtga tacagatcac cgttcccttg tactgctgca aatgctgctc cagccagtcg
     1321 atcgactcgg catcgaggtg gttggtcggc tcgtcgagaa gcaacacgtc gggttcctgc
     1381 agtagcaggc ggcacagtgc cacccggcgg cgttcacctc ctgaaagttc acggaccggc
     1441 tgttcttcag gcggacagcg aagggcatcc atcgcacgct ccaggcgatt atcgatgttc
     1501 cacgcgtcga cggcatccag cttgtcctgc aattctgcct ggcggtcgaa cagcttgttc
     1561 atcttttcct catcctcgta atattcgggc aatccgaact tcaggttgat ctcttcatat
     1621 tccgccagca ggtccatcac cggctgcact ccttcacgaa cgatttcgat caccgatttg
     1681 tcttcatcca gtttcgggtc ctgttccagg tatcccaccg agaaactttt atcggagact
     1741 acttcgcctt ggtactcctt ttcaatccca gcaataatct tcagcagggt agatttaccg
     1801 gaaccgttca gcccgatgat gccaatcttg gcaccgtaga agaaggagag gtagatatcc
     1861 ttcaacacct gtttgtgggg agggaacgtt ttacttaccc ccaccatgga aaaaataatt
     1921 tttttatcgt cagccatatc tgtttttagt cagtttattc aatattttat caatgtcccg
     1981 tccctctaag tcgatataaa tcaacatttt tcaacccgac ctttcgtaac ggagcacaaa
     2041 gatagtaaaa atgacggaaa aagggcaaaa cctgaagaca agcatgaatg aagaggccgt
     2101 ttccgtatgt tcgtttaaaa ttgtaatttt acacggttta aaattctgat tgcaggaagc
     2161 gttggaataa cagaatggaa cgaatggatt acagggacac cgtttttatt tcagtggtag
     2221 aaaacctcag tttctcgaaa gcagccgatg aactgaacat cagtcagcct gcggtaacaa
     2281 ggcacatcaa agagttggaa gagaggtatc acaccaacct tttcgaacgt aaaggcaaca
     2341 agatttacct gacgcgggca ggagagaaag tataccacac gtttaagctg atcgaccggc
     2401 agtaccgtga gctcgatttt gaggtagggg aactgcaaaa caccgttgcc ggcgagttca
     2461 gaataggtgc gagctcgacc atcgcacaat atgtgatacc caaagtgatc gcatcgtttc
     2521 ataagcggta tccaacgatt aaaatccatt taatgaacgg gaattcgttt gaaatggaga
     2581 aacagttgat ggagaacagc gtggacatag cgcttgtaga aaacacctca tcccaatcgg
     2641 atatccggta taaaaagttt ctcgacgatg aactgatcgt ggtcaccggc tccggaagcg
     2701 tttatgctaa acgggaaacc atcacccaag acgagctgtc gcgactcccg atggtgttaa
     2761 gggaacaggg ttccggcaca ctggaagtca taaaagaggc cttttcccgg cacgggatag
     2821 atagtgaaca cttccataca cccatccacc tgggcagcac ggaatccata aaaaatttcc
     2881 tgctcgattt cgatggcgtg gctattgtat cggaaaaagc ggtctccggc gaactgcaac
     2941 tgaaaaccct tgtaaaatta aaggtttccg gattttccat tcctcgccaa ttccgcatag
     3001 cctacaagct gggacataaa agccgccagg ttgaactgtt tgaaaatttc ctccttaatt
     3061 ataacatata gttatacatt ataagaataa agcatttgtt tatattatac aacgatacta
     3121 cttttgtcgc cttaaagtat cggaatgaat aataaaagtt ggctttattg ggtggttatc
     3181 tgtttttgtt ttctaccttt tgtttcaccg gcggttgcac tctgcatagg cattgttttt
     3241 tcttttttgg gaatcaggca cgattccatt caccggtata cctcaagggt tctacaggta
     3301 tcgattgttc tcctgggctt cgggatggga ctggaagaag tgatcactgc gtcaaaaagt
     3361 ggcttcgtac agacgctcat ctcagtatcc ctggtgatga ccggcgggat tgtccttggc
     3421 aggctcttga gggttgaaaa aaacacaaca ctgctgattg ccgcggggac ggcaatttgc
     3481 ggaggaagtg ccattgcggc tgtggcaccc atcatcaaaa gtgagaatta tcaaaattcg
     3541 tttgccctga tcgtggtgtt tgtcctgaac gccatagccc tgctgctttt tccctttatc
     3601 gggcaacggc tcgggttgag ccaggaagtt tttggcaact gggcagccat cgccatacac
     3661 gataccagct cggtagtggg agcaggagcc atctacggag agaaggcact gcaggtggcc
     3721 actacggtga agctgatacg tgccttatgg attatcccgc tctccatcgt gctggctttt
     3781 ttcagcaaaa acggcgacaa gcgttcaatc aaattcccct ggtttatcct gctctttgtg
     3841 gcagccatat tgtttgccaa tatttttcct gcttttgagt ccagctatgc ccatttcagc
     3901 tggctgggaa gaagggcaat ggttgtcgca ctgtttctca tcggttccaa catcaccctc
     3961 catcaaatca aacagtcggg aacccggtgt tttctgttgg gcgttacatt atgggtggtg
     4021 acggtagcct tctcactttt cctgttaacg cggtaatcag gcttcttgac tacaagcaac
     4081 ggttactcac cggaagtaac gcatcagttt atttcgacga tctcaaaatc aaaaaattac
     4141 agttttttga tacaaataat ttcgttaaaa taaaaacatt tcgtattttt gcaacttgat
     4201 tagaaagata gtatttagga acattcttaa caaataaaaa ttcaaaaaat tatgattaaa
     4261 gtaggtatca acggattcgg acgtatcgga cgtttggttt tccgggcagc tcaaacaaga
     4321 gatgacattc aggtggtagg aatcaacgat ttgcttgatg taaattacat cgcatatatg
     4381 ttgaaatacg atactattca cggccagttc accggaacga ttgatgtgaa agacggccat
     4441 ttggtggtaa acggaaaaac catccgcgta acagctgaaa aaaacccggc tgatttgaaa
     4501 tggggagaag ttggtgcgga ttacgttgtt gaatctaccg gtttattcct ttctaaagac
     4561 aaagctcagg gccatattga tgcaggtgca aaatatgtgg ttatgtcggc tccttctaaa
     4621 gacgacacac gcatgtatgt atgtggcgtt aacgaaaaca cctataccaa aggtgagcag
     4681 tttgtgtcaa acgcttcatg tacgaccaac tgtctggctc ctatcactaa agtactgaac
     4741 gacaaattcg gcgttctcga aggtttgatg actaccgttc actctacaac agccactcaa
     4801 aagactgttg acggcccatc cgctaaagac tggagaggcg gccgcgcagc atcgggcaat
     4861 atcatcccat cttccacagg agcagccaaa gcagtaggaa aagttattcc cgaactgaac
     4921 ggaaaactga ccggtatgag catgcgcgtt cctaccctga acgtttctgt agttgacctg
     4981 actgtacgtc tggcaaaaga aacatcttac gcagaaatct gcgctgctat gaaagaagct
     5041 tcagaaggtg aattaaaagg catcctcggt tacactagtg acgacgtggt ttcttccgat
     5101 tttatcggtg atgcacgcac gtcaatcttt gacgaaaaag caggtatcca actaagcccg
     5161 actttcgtta aagtaatcag ctggtatgac aacgagtggg gttactcaaa caaagtactc
     5221 gacctggttg cacatatggc aaaagtaaac ggataatcgt ttattaacac atacgcaaaa
     5281 agcgctaccc gaaaaggtgg tgcttttttt gttgccgttt cacaaaaaaa agaaaggccg
     5341 gccggagtat tccgatcagc ctttttacct cttttccggt atatgttaag ccggatgaat
     5401 tgcttctgta gggcaaactt ctgcacaagt accgcaatcg gtacaaacat cgggatcaat
     5461 cacgtagata tcaccttcag agattgcctc aaccgggcat tcatctatac aggttccaca
     5521 agcaatacaa tcttcattaa ttacgtaagc cataataaat aatttgttag ttaatgtaaa
     5581 tggatatttg attgttgatg caaaggtaga aagttttttg caatctgaaa aaagattccc
     5641 catatctttc caattcccac acaatatccg g
//
LOCUS       DAOSDR010000212         5612 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrimonas sp. isolate AMR_MDS_1826
            NODE_5163_length_5612_cov_3.802951, whole genome shotgun sequence.
ACCESSION   DAOSDR010000212 DAOSDR010000000
VERSION     DAOSDR010000212.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863355
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrimonas sp. (wastewater metagenome)
  ORGANISM  Petrimonas sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Dysgonomonadaceae; Petrimonas.
REFERENCE   1  (bases 1 to 5612)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5612)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:10:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,720
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 35
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5612
                     /organism="Petrimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5163_length_5612_cov_3.802951"
                     /isolate="AMR_MDS_1826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2023866"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            157..756
                     /gene="yihA"
                     /locus_tag="PLI30_08885"
     CDS             157..756
                     /gene="yihA"
                     /locus_tag="PLI30_08885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007657110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HOI79705.1"
                     /translation="MLIKSAEFIISNTDYLKCPQDGKPEYAFIGRSNVGKSSLINMLT
                     NRKGLAMTSSTPGKTMLINHFLINGEWYLVDLPGYGYARRGKENRDRLQQIIEDYVLE
                     REALTSLFVLVDSRHEPQKIDLEFMEWLGENGVPFGIVFTKGDKQGPVRLQQNVDAYK
                     QKLLERWEELPPVFVTSSEKKLGREELLDYIEKINKLLR"
     gene            complement(784..1965)
                     /locus_tag="PLI30_08890"
     CDS             complement(784..1965)
                     /locus_tag="PLI30_08890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019540483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator"
                     /protein_id="HOI79706.1"
                     /translation="MIKILVLIDSATEFSRRFLSGLVRYAHENGPWIFYRLPLYYKML
                     YGEEGVLEQIKEWGIDAVIVQWEYREIKFLKKLDIPVFLQNYEDNADWFSKISGDYME
                     VGAIAANFFAQKRFKNFAFYGNKNFLWSKGRAEGYRIEVEKLGGNYYYFESEYLTNSQ
                     WSRSHIKLNKWLSSLPKPVAVFACDDYFALQVAEMCKINHIDIPNEISLLGVDNDELI
                     CNLSYPSISSIVTDDENIGYVAGKKLHKLIINKKNIPFNIIKKPIRIEQRQSTEKYNI
                     ANKYILKIIEHLEKNFASDLSIDELTQMVPLGRRSLEKKFKEAMGTSIYQFIIDKKIE
                     FFSYKLLTTQESSLDIAIEAGFNDVRNAYRIFKKNTGHSPSGFRKKFSTRDKPPLSPT
                     D"
     gene            2255..3025
                     /locus_tag="PLI30_08895"
     CDS             2255..3025
                     /locus_tag="PLI30_08895"
                     /inference="COORDINATES: protein motif:HMM:NF013356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HOI79707.1"
                     /translation="MTTEIKQFKKENLTVRIFTDEASAGKGSADFVAKHLNYAIRAKG
                     HANLILATGASQFAFIEAIKTLPVDWSKITVFHLDEYRDLPETHPASFRKYLKERILD
                     EVKPYQVNYIHGDAKDIEREIARYEELLKAAKIDVACIGIGENGHIAFNDPEVADFND
                     PQLVKIVQLDDISRRQQLGEGWFSTLEEVPREAVSLTIPAIMRCNVISCMVPDKRKAN
                     AVYNTLNAEITTACPATILRRHPYTVLFLDGNSASQLK"
     gene            3040..4491
                     /locus_tag="PLI30_08900"
     CDS             3040..4491
                     /locus_tag="PLI30_08900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019540488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOI79708.1"
                     /translation="MQINRRNFLKAAGVATVSAMLPTKGISREIPAEKQQSSKSLEKI
                     YREAYKKHSQKFNMCGYTAPKLETVRVGFVGVGNRGSAAVERISRIDGVKIVAICDEL
                     SEHAEKAKARIQTPGHPALVYSGKEDSWMEMCRRDDIDLVYVAAPWEWHAPIGVYSMR
                     QGKHVALEIPVATTVEDCWQLVETSEETKKHCVILENCCYDFFELLTLNLARNGFFGE
                     IVHAEGGYIHEIGESLFFRGKPGRSWRLKENMRRNGNLYPTHGLGPVAQIMNINRGNR
                     MEYMVSMSSDDFLMNQYSQELSLKEDYFKQFEGASFRGNMNSSIIKTIKGQTILIQHD
                     ITTPRPYSRLHTISGTKATAQKYPLPPRLSMAGDHKEWLSEDKFEEIERRYNPPIIKK
                     MQDIAKEIGGHGGMDFLMDWRLIDCLRNGLPVDMDVYDAASWSVIGPLSEWSVANRSA
                     PIDIPDFTNGAWKKNQPHDISLKHGGTTGVVTK"
     gene            4502..>5612
                     /locus_tag="PLI30_08905"
     CDS             4502..>5612
                     /locus_tag="PLI30_08905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019540489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOI79709.1"
                     /translation="MNKKVFYISMGILAVMYFVIGLVSWVNAILIPYFKIALELTHFQ
                     SYFVTFAFYIAYFVMAVPSGLLLNRTGYKKGIMYGFLFLSLGALIFIPAALMRQYAVF
                     LVGLYCLGTGLAILQTAANPYVTIVGPIDSAAQRMSVMGIFNKSAGIIAPLLFAAVVF
                     KSTDDGTFALLESGALSETEKNIILQELIRRVIMPYSILAVLLVLVGFGIRYSVLPEL
                     EEKEDKAQEQNSDLPPVAGTPRKSLLDYPYLVFGAIAIFLHVGTQVVAIDTIINYAGS
                     MGIGLLDAKFFPSYTLLCTIIGYLLGILLIPRFIPQKAALKFCTVLGFLLSLGVVFAN
                     QPVSLFGHTANISIWLLASIGFPNALIYAGIWPLSI"
BASE COUNT         1636 a         1113 c         1249 g         1614 t
ORIGIN      
        1 cagttatctg ttttgttttt gaaaccgtga tgaaacaggt tttgggctac caggtgggtt
       61 atgaactctt gcttgtcaat gctgcactta ccggaaccgg cctttggata ttgtcgttaa
      121 acaactataa acctttaacc ctcaacccgt aaccctatgc ttatcaaatc tgccgaattt
      181 atcatcagta acaccgatta cctgaagtgt ccgcaggacg gaaagcctga atatgctttt
      241 atcggacgtt ccaatgttgg aaaatcatcg cttatcaata tgctgaccaa ccggaaagga
      301 ttggccatga cctcatccac acctggaaaa acgatgctga tcaaccattt tctgattaac
      361 ggagaatggt atctggtaga tttaccggga tatggctatg cccgacgtgg aaaggaaaac
      421 cgggatcggt tgcaacagat aatcgaagat tacgtgctgg aacgtgaagc gctgacctca
      481 ctttttgtat tggtggacag ccgccacgag cctcagaaaa tagatttgga gtttatggaa
      541 tggctgggcg aaaacggtgt tcccttcggc attgttttta ccaaaggcga taagcaggga
      601 cccgttcgcc tgcagcaaaa cgtggatgct tacaaacaga aattgctgga aaggtgggaa
      661 gaacttccac ccgtctttgt tacctcctcc gaaaaaaaat tgggtaggga agagttgctc
      721 gattatattg agaaaataaa caaactcctt cgttaaataa ttgaaaacct tattgatatt
      781 cgattaatct gtcggagaaa ggggcggctt atcccgtgtc gagaattttt tacggaaacc
      841 agaaggacta tgcccggtgt tctttttgaa aatccgatac gcatttctca catcgttaaa
      901 cccggcctca atagcgatat ccaacgagct ttcctgagtt gtcaataatt tataagagaa
      961 aaattctatt ttcttgtcta ttataaattg ataaatggag gtgcccatgg cttctttgaa
     1021 ctttttttca aggcttcttc ggcccagcgg aaccatttgt gtcagttcat cgatggagag
     1081 atcagaagcg aaattttttt caagatgttc aattatcttt aagatatatt tgtttgcaat
     1141 attgtatttt tctgtagatt gccgttgttc aatacgtatt ggcttcttga tgatgttgaa
     1201 aggaatattt tttttgttta taatcagttt atgaagtttc ttgccagcaa catatccgat
     1261 attttcgtca tcggtcacta tggatgagat ggacggatag gataagttac agataagctc
     1321 gtcattatct acccccagta aagatatttc attcgggatg tcgatatggt ttattttaca
     1381 catttcggcc acctgcagag caaaatagtc gtcacaagca aaaacggcaa ccggctttgg
     1441 aagggaagat aaccatttat ttaattttat gtgacttcgg ctccattgac tgttggttag
     1501 atattccgat tcaaaatagt aatagtttcc tcctaatttt tctacttcga tacggtaccc
     1561 ctccgctctg ccttttgacc acaaaaaatt tttattgcca taaaaagcaa aattcttaaa
     1621 ccttttttgg gcaaaaaaat tagcggcgat cgcacccact tccatatagt caccggaaat
     1681 ttttgaaaac caatcggcat tatcttcata gttttgaaga aatacaggaa tatccagttt
     1741 ttttaaaaat ttaatttcac ggtattccca ttgaacaata accgcatcga tcccccattc
     1801 tttaatttgc tctaagaccc cctcttcacc gtacaacatt ttataataca gaggcaaccg
     1861 gtaaaatatc caagggccgt tttcatgggc ataacgaacc agcccggaca gaaaacgacg
     1921 gctaaactca gtggcagaat ctataaggac aagtatttta atcattgctt attgttggaa
     1981 tttatttacc cgaatttaaa tcaacgttct catagtaaca ctagaaaccg tagaatgttc
     2041 cataattgtg attatttcta tgaatatatt cgtgataagc cacattggat tcgttaatta
     2101 tatcataaga tgttgtaacg gagactatct ttgctaaaag ataaaccggt tacaccagaa
     2161 tacggataag gttcatggga aaaaccggaa aagggtattt atcttgaagc agatgaaaat
     2221 gtttattgat gaaactttat aattaagata agaaatgaca actgaaatta aacaattcaa
     2281 gaaagaaaat cttacggttc gtatttttac ggacgaggct tctgccggaa aaggcagcgc
     2341 ggactttgtg gccaaacatt tgaattacgc tattcgggcc aaggggcatg ctaatctgat
     2401 cttagccacc ggcgcatcgc aatttgcctt tatcgaggca ataaaaacgc tccccgtcga
     2461 ttggagtaaa attaccgtat tccatttgga tgagtatagg gatttgcccg aaacccatcc
     2521 ggccagtttc cgaaaatacc tgaaagaaag aatcctcgat gaagtaaaac catatcaggt
     2581 taattacatt catggcgatg ccaaggatat tgaacgtgaa atagcccgct atgaagaact
     2641 gttgaaagct gccaagattg atgtagcatg cataggtatt ggggagaatg ggcatatcgc
     2701 ctttaatgat cccgaagttg ccgatttcaa cgatccgcaa ttggtgaaaa tagtgcagtt
     2761 ggatgatatc tcacgccggc agcaattggg agagggatgg ttctctactc tcgaagaggt
     2821 acctagagaa gctgttagcc tgacaattcc tgccatcatg cgttgtaacg taatcagttg
     2881 tatggtgccg gataagcgaa aagccaatgc tgtttacaat acactgaatg cggaaataac
     2941 gacggcatgc ccggcgacga ttttaaggag gcatccttat acagtactgt ttttggatgg
     3001 aaactccgcc tctcaattaa aataaaaata gatttctcca tgcaaatcaa tagaagaaat
     3061 ttccttaaag cggcaggagt tgcaactgta tctgccatgc tgccgaccaa agggataagc
     3121 cgtgaaatac ctgcggagaa acaacagtcg agcaagtctt tagaaaaaat ataccgtgaa
     3181 gcctataaaa aacattcgca aaaatttaat atgtgcgggt atactgcacc caaactggaa
     3241 acagtaagag tggggtttgt aggcgtgggg aacaggggaa gtgcggccgt agaaagaatc
     3301 agccgcattg acggagttaa aattgtggcc atttgcgatg aactttccga gcatgcggag
     3361 aaagccaaag cgcgtattca aacacctgga catccggccc ttgtttattc gggaaaagaa
     3421 gatagctgga tggagatgtg ccggagagat gatattgatc ttgtatatgt tgccgccccc
     3481 tgggaatggc atgcacccat tggggtatat tccatgagac aaggcaagca cgtggcactg
     3541 gaaatcccag tcgcaaccac agtagaggat tgttggcaat tagtagaaac ttcagaggaa
     3601 accaaaaaac attgtgtgat tctggagaac tgttgttacg atttttttga attactgact
     3661 ttgaatctcg cacgcaatgg tttttttgga gaaatagtgc atgcggaggg aggatatatt
     3721 catgaaatag gggagagctt attcttcagg ggaaagcccg gaagaagctg gaggcttaag
     3781 gaaaacatga gaagaaacgg aaatctctac cctacacatg gattagggcc cgtggctcaa
     3841 atcatgaata tcaatcgggg gaaccggatg gaatatatgg tgtcgatgtc atccgatgat
     3901 tttttgatga atcaatattc ccaagaacta tccctgaagg aagattattt taagcagttt
     3961 gaaggagcct cattccgagg gaacatgaat agctcgatca tcaaaacaat caaagggcaa
     4021 acaatcctta ttcagcacga tatcaccacg cctcgccctt attcacgatt acataccata
     4081 agcgggacaa aagcaacagc gcagaaatat cctcttcctc cccgcctatc catggctgga
     4141 gaccacaaag agtggctgtc tgaagataaa ttcgaggaaa ttgaacggag atataatcca
     4201 cccatcatca agaaaatgca agatattgcc aaggagattg gcgggcacgg aggaatggac
     4261 tttttgatgg attggagatt gattgattgt ttaaggaatg gcctgcctgt cgatatggat
     4321 gtgtacgacg cagcgtcgtg gagcgtgata ggtccgttaa gcgaatggtc ggtcgccaat
     4381 cgatcggcac cgatcgatat cccggacttt acgaatggag catggaaaaa aaatcaaccc
     4441 catgatattt ctttaaagca tggaggaact acgggcgttg tcaccaaata atatttcctt
     4501 tatgaacaaa aaagtttttt atatttcgat gggtattttg gccgtgatgt attttgtcat
     4561 cggcttggtg tcatgggtta acgccatcct tatcccttat tttaagattg cccttgaact
     4621 gacacacttt cagtcctatt tcgtcacttt tgccttttat attgcctatt ttgtgatggc
     4681 cgtcccgtcg ggtttattat taaacaggac gggatataag aaaggaatca tgtacggatt
     4741 tttattcctg tcgttaggtg cccttatctt tatcccggca gcacttatgc ggcaatatgc
     4801 ggtttttctt gtgggattgt attgcctcgg aacagggctt gctatccttc aaacggcagc
     4861 aaacccttac gtcaccatag tcgggccgat agatagtgct gcgcagcgaa tgagtgtgat
     4921 gggcattttt aacaaatctg caggcattat tgctccttta ctttttgcag cggtagtgtt
     4981 caaatcgact gatgacggaa cttttgcgtt acttgagtca ggagcgcttt ccgaaacgga
     5041 gaaaaacatt attcttcagg aactcatcag gagggtaatt atgccctatt ctattcttgc
     5101 cgttcttttg gtattggtgg gtttcggtat aaggtattca gtactgccgg agttagaaga
     5161 gaaagaggat aaagctcaag agcagaatag tgatttaccg ccggttgcag gcacaccaag
     5221 aaaatctctt ttggattatc cctacctcgt ttttggagct attgcgattt ttctacacgt
     5281 aggtacccag gtagtggcaa tagataccat catcaattat gccggttcga tggggatcgg
     5341 tttgttggat gcgaagtttt tcccttctta tacgcttctg tgtacaatta tcggttattt
     5401 gctgggtatc ttgcttatcc cccggtttat tccgcaaaaa gccgctttaa aattctgtac
     5461 cgtactgggg tttcttcttt ctttaggggt agtgtttgct aaccaacccg tttcactatt
     5521 cgggcataca gcaaacatct cgatatggct cctagcctca ataggatttc ctaacgcatt
     5581 gatatatgcc ggtatctggc ccttatcgat tc
//
LOCUS       DAOSDR010000213         5581 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrimonas sp. isolate AMR_MDS_1826
            NODE_5196_length_5581_cov_3.618531, whole genome shotgun sequence.
ACCESSION   DAOSDR010000213 DAOSDR010000000
VERSION     DAOSDR010000213.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863355
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrimonas sp. (wastewater metagenome)
  ORGANISM  Petrimonas sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Dysgonomonadaceae; Petrimonas.
REFERENCE   1  (bases 1 to 5581)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5581)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:10:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,720
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 35
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5581
                     /organism="Petrimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5196_length_5581_cov_3.618531"
                     /isolate="AMR_MDS_1826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2023866"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1945)
                     /locus_tag="PLI30_08910"
     CDS             complement(<1..1945)
                     /locus_tag="PLI30_08910"
                     /inference="COORDINATES: protein motif:HMM:TIGR04056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0071702 - organic substance transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SusC/RagA family TonB-linked outer membrane
                     protein"
                     /protein_id="HOI79710.1"
                     /translation="MKLTPLERTLHLKGCSIRRLLPVFLMVFLCFTGNTAFAQNMRVT
                     GKVVNADTGEAIPGASVVVEGTLNGIATDVDGNYTLQVPVNGTLVFSFIGYAEVKVKV
                     SGQSTINVQLKQSALEMEELVVIGYGAVRKSDVTSSISTVDSKDIVKTPAASITQALQ
                     GNASGVLIMNTAADPGSDAKIRIRGGSSISGSNEPLIVIDGFPSDASILSMLNPNDVK
                     SMEILKDAAATAIFGSRGANGVILISTKSGTEGKAKINVDAKVGLATPRRMLEMMDAP
                     EWQRYADDALTMTSNPDKKNSYDFAPELLETTNFQDLLIRNVAQRHDYNISVSGGGKE
                     STYYVSAGYLKEDGLLNNSSSDRMSTKTKLTMKLHEKVKLQMNLSTSRRQTQSIAGGD
                     TGAMLRTLMIRPKKPTGNFEDGVYRDPDTGEILSIQAETARAMNSNLWKKSFNADVSG
                     ALTWNMIKGMNLNVSGSYSYTDKLGYRYVPRSIYLKYSDIQKNNKAERSSDQTIKWVN
                     ENTLNYVRSFGRHNFNLVAGQSWERRMDEGFNASVTEFGTDHYMWNNLGAGLQTPVIG
                     SGKSEYGLVSFFSRVIYNYDKRYLATLTWRSDGSSRFGEDRKFGNFPSASFAWNIHNE
                     NFMKGNHTFSNLRFRLSWGITGND"
     gene            2381..>5581
                     /locus_tag="PLI30_08915"
     CDS             2381..>5581
                     /locus_tag="PLI30_08915"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HOI79711.1"
                     /translation="MKAIFFRTAPVILLLCLCFAGSFAETENNLFVRIPNNPVSNAIT
                     ALAQDQNGFLWLGTDKGLVRFDGIEYKIFRGENFDSVRMINSYIRNLYLDSKGILWIG
                     TNEGAVIYNPEAGRFIRLNIVPANTTVKSFMELPGEEVLIGTNKSLLIANRKGEVSVL
                     SSGGLNPLAGENVTYMAKDLYGNIWLANNNGFTRIDIPDTLSAQKDNLVFTFYPQKFN
                     LGYFSIDPMNNIWFCDGETIKRTKATQNDRLEKIETIRTEFDSRAIFHDKNERMTMLN
                     RRYESIHLLFYDETGRIVSEKKYQLNPLSPNHMSDNISSIIRDKKGNFWFGTRDGLFV
                     YDDSSLKNFHTITEKKGSTDGLSHSVISEILEDSQKNIWIGTSIGLNKLSFPNGKPYI
                     EQFSDSHDINSFRLNNRVQALAEGDDGILWVGSKLGIAYFDPEKKHFFARAETDNFLS
                     KINAMFVRTMFKDKQGRIWIGFVYGGLICFDSASGKFLKITTPRNDFNKQDIWAVLED
                     IDGSIWFGSKRDGLFRFQPDSIQSSGMVQHMRQYNVSGRKNSSTSTDWITSLFLDFNR
                     SLWVGTSRGLYKYNRDSNTFQRIILSSPSEEPYICGIKQDLQGNYWISTTTGVHRYND
                     QKGFSTFFEFNQGDFARVEYSFGSCLTDDGKILLGGIKGVVYFDPSKIAVDTTLQNRI
                     FITDFRIGNQEITPDGIHLKNDINTDNRITMKYPENQFTLAFSSLHLNNPQYIRFAYM
                     LEGVDDNWIYTEWNRNYAGYTNLSPGKYRLKIRSTNETGSWLDNIRTIDLHILPPWYM
                     TYWFYALAALICLTVLFFIFRILYSRNHYRQAEQINAIKQTYFINVTHSFKTPLMLLQ
                     TPLNQLIRHGDQMTIDERDMMLNLMNKNVKRISNLIRQVTEFRKIEQKKTSLYLVEMN
                     IIPFLNEICNSFQPMFHSKQIPLNFESNIPMVMVTIDPEKIETILFNLLSNAYRFTPE
                     GGKVTVRCMLEPTDYKLYISVSDTGIGIEPAHRQKVFERFWKAKTTENNFYSNGAGIG
                     LSLSKELVELHHGEIFLQSHPGAGSTFTFTLRLGSKH"
BASE COUNT         1556 a         1329 c         1186 g         1510 t
ORIGIN      
        1 cgtcgttgcc agttatcccc cagctcagac ggaaacgaag attagagaag gtgtgattgc
       61 ccttcatgaa gttttcattg tggatattcc aggcaaacga agccgagggg aaattcccga
      121 atttgcggtc ttccccaaaa cgggatgatc cgtcggaacg ccaggtcagt gttgccagat
      181 agcgtttgtc gtagttataa atcacccggc tgaaaaaaga gaccaggcca tattcagatt
      241 taccggaacc aattaccgga gtctgaagcc ctgccccaag attattccac atataatgat
      301 cggtaccaaa ttcggtgacc gaagcattga atccttcgtc catccttctt tcccagcttt
      361 gtccggccac caagttgaaa ttatgccgcc cgaaactccg aacatagttc agggtatttt
      421 cattcaccca ttttatggtc tgatccgacg aacgctccgc tttgttgttt ttctgaatat
      481 ccgaatattt caggtaaata cttcgcggca cgtaccggta acccaattta tccgtatagc
      541 tgtaactccc gctaacattc aggttcattc ctttaatcat gttccaggta agggctccgc
      601 tgacatcggc gttgaaactc ttcttccaga ggttggagtt catcgcgcgg gctgtttcgg
      661 cctggatact cagaatttcg cctgtgtcgg gatcgcggta cactccgtct tcgaaatttc
      721 cggttggttt tttcgggcgg atcatcagcg tacgcaacat cgctccggtg tctcctccgg
      781 caatcgattg tgtctgtctg cggctggttg aaaggttcat ttgtaactta accttttcat
      841 gcagtttcat ggtcagtttt gtcttggtcg acatccggtc cgaactgcta ttgttcagca
      901 ggccatcctc tttcaggtat ccggcagaca catagtaggt cgactctttc cctcctccgg
      961 agacagagat attgtagtcg tggcgttggg ccacgttacg gatgagaaga tcctgaaaat
     1021 tggtcgtttc caacagctcc ggagcaaaat cgtagctgtt tttcttatcc ggattactgg
     1081 tcatcgtcaa agcatcatct gcataccgct gccattccgg agcatccatc atctcaagca
     1141 tccgccgggg agtagccaag ccgactttgg catcaacatt gatttttgct tttccttctg
     1201 ttccgctttt ggtggaaatc aggatcaccc cgttagctcc ccgcgaaccg aaaatagcag
     1261 ttgcagcagc atctttgaga atttccatcg attttacatc attcggatta agcattgaca
     1321 gaatagaagc gtcgctggga aagccatcaa ttacgattaa cggttcgttg cttcccgaaa
     1381 ttgagctccc tccgcggatc cgtatctttg catcagatcc gggatcggcg gcagtattca
     1441 tgattaatac ccccgaagca tttccttgca aagcctgggt aattgaagct gcgggagttt
     1501 tcacgatgtc tttgctgtcg acggttgaga ttgagctggt cacatcactt ttccgcacag
     1561 caccgtaacc aataacgacc aactcttcca tctccaaagc agattgtttt aactgcacat
     1621 tgatcgtaga ttgtccgctc acttttactt ttacttctgc ataccctata aaactaaaaa
     1681 caagggtacc gttgaccggt acttggaggg tgtagtttcc gtccacatct gttgcgatcc
     1741 cgttgagggt accttccacc accacacttg ccccgggaat agcttctccc gtatcggcat
     1801 tcaccacttt tccggtaacc cgcatgtttt gtgcaaaagc tgtattgccg gtaaaacaca
     1861 gaaataccat taaaaacacc ggcaatagcc gtctgatcga acaacctttc aagtgcaatg
     1921 ttctttccaa aggcgttagt ttcattttca gattgattta agttgtttta ttaatttcga
     1981 ataactgatc cgatgtaacg gaacaaattt agatttccgg gaagcggata ttgttgaaat
     2041 tatgctggat tatgtcggtg gtgaccggtc gggaaagtat tacgatgaac tcggcaggct
     2101 gatttcacct ccgaatgaaa aatctttttt tgctcctgtc aattgaggtc ccaaaaacag
     2161 catccaccct ttcaaaaccc aaccaacaaa caagtcacca acaacttatc accattaaaa
     2221 aaaggtctaa acttaacgtt cgccatcaaa aagacgaagg aacgctcttc gggaaaggtt
     2281 taaaatctga cgaaaaatgt gtaaaaattt tgctccaaat gcctcttact tttatttttc
     2341 tatcttcgtg aacccgcatt tttaatgggc gggcatgatt atgaaggcca tcttttttcg
     2401 aacggcacca gtgatactct tgctgtgttt atgctttgcc gggagctttg ccgagacaga
     2461 aaacaatcta ttcgttcgca ttcccaataa tccggtcagc aatgcaatca ccgcactggc
     2521 tcaagaccag aatgggtttt tgtggttagg tacggacaaa ggcctcgtcc gtttcgacgg
     2581 tatcgaatat aaaatttttc gtggagaaaa ttttgattcc gtacgaatga ttaattctta
     2641 tatccgaaat ctttacctgg attcgaaagg aatactttgg ataggaacaa acgagggcgc
     2701 cgttatctac aacccggaag cgggaagatt catcaggttg aatatcgttc ccgccaatac
     2761 tacagtaaaa agtttcatgg aattacccgg ggaagaagtg ttaatcggca cgaacaaatc
     2821 gcttctgata gctaaccgca aaggagaggt atcggttctc tcttccgggg ggctgaatcc
     2881 tttagccggg gagaatgtta cgtacatggc aaaagacctc tacggcaata tctggcttgc
     2941 caacaataac ggttttacaa ggatcgacat tccagatacc ctgtctgcgc aaaaggacaa
     3001 tctggttttt accttctatc cgcagaaatt caatttgggt tatttctcca tcgacccgat
     3061 gaacaacatc tggttttgcg acggggaaac gattaagcgc acaaaagcca cacaaaacga
     3121 ccgccttgaa aaaattgaaa ccatcagaac ggagtttgac agccgggcaa tattccatga
     3181 taaaaatgaa cggatgacca tgctgaaccg gcggtacgaa agcatccacc tgttatttta
     3241 cgatgaaacc ggccgcattg tatctgaaaa aaaataccag ctaaacccac tctcccctaa
     3301 ccacatgagt gataacatct ccagtataat tcgtgacaag aagggtaatt tctggtttgg
     3361 aacccgcgat ggattgtttg tatacgatga ctcttctttg aaaaatttcc atacgatcac
     3421 agaaaaaaaa gggagcaccg acggtttatc ccacagtgtg atttccgaaa tcttagaaga
     3481 cagccaaaaa aacatctgga taggcacctc catcggcctg aacaaacttt cgttcccaaa
     3541 cggaaaacct tatatcgaac aattttcgga cagccacgat atcaacagtt ttaggctaaa
     3601 caatcgagtg caggcgcttg ccgaaggaga tgacggtatt ttatgggtgg ggtctaaatt
     3661 aggtatcgct tattttgatc ctgaaaagaa acactttttc gcaagagcag aaacggataa
     3721 tttcctttca aagatcaatg caatgttcgt tcgcaccatg ttcaaagata aacaggggcg
     3781 aatctggatc ggatttgttt atggaggttt aatttgtttt gattcggctt ccggtaaatt
     3841 tctaaaaata acaacccccc gcaacgactt caacaaacag gatatctggg ccgttcttga
     3901 agacatcgac ggatcaatct ggttcggaag caaacgggac ggattattca ggtttcagcc
     3961 cgattccatt caaagcagcg gcatggtcca acacatgagg cagtataacg tatcggggcg
     4021 gaaaaacagt tctaccagca ccgattggat cacttcgtta tttctcgatt tcaaccggtc
     4081 tctctgggtt ggcacttccc gcggccttta caaatacaac cgtgactcca atacctttca
     4141 acggatcatc ctctcttctc cttccgaaga gccttacatc tgcggaatca agcaggacct
     4201 ccagggaaac tactggataa gcacaaccac tggtgtccat cggtataatg accaaaaagg
     4261 tttttcaacc ttttttgagt ttaatcaggg cgattttgcc cgtgtcgaat attcgttcgg
     4321 aagttgtttg accgatgacg gaaaaatatt gttgggcggg atcaaagggg tcgtttattt
     4381 tgatccgtca aaaattgcag tggacacaac cctgcaaaac cggattttca taacagattt
     4441 ccgtatcggt aaccaggaaa tcactcctga cgggatccat ttaaaaaacg acatcaatac
     4501 cgataaccgg ataacaatga aatatccgga gaaccagttt acccttgctt tctcctccct
     4561 gcatctcaac aatccgcaat atatccgttt cgcctacatg ctggaaggag ttgacgacaa
     4621 ctggatctac accgaatgga accggaatta tgccggatac accaatttat cgccgggaaa
     4681 atacaggctg aagatccgtt cgaccaacga aacaggaagt tggctggaca atatcagaac
     4741 gatcgattta cacatcctcc cgccgtggta tatgacctat tggttttacg cactggctgc
     4801 cctgatatgc ctgactgttt tattcttcat tttcaggatt ctctattccc gaaaccatta
     4861 ccggcaggcc gagcagatca atgcgataaa acaaacctat ttcatcaatg ttacgcacag
     4921 tttcaaaaca ccattaatgc ttcttcaaac cccgctgaac cagttaatcc ggcacggaga
     4981 ccagatgacc attgacgaac gggatatgat gctaaacctg atgaataaaa atgtaaaacg
     5041 aatctccaac ctgatcaggc aggtaaccga attccgtaaa atagagcaaa agaagacctc
     5101 tctttatctg gtggagatga atattatccc ttttctgaat gaaatatgca attcttttca
     5161 gccaatgttt cattccaaac aaatcccgct gaactttgaa agcaatattc ccatggtgat
     5221 ggtcaccatc gatccggaaa aaatagagac catcctcttt aatctgttgt caaacgctta
     5281 ccgtttcacc ccggaaggtg gcaaagtaac ggtcagatgt atgttggagc cgacagatta
     5341 caaactgtac atctcggtgt ctgatacagg aattggcatc gaaccggcgc atcgccaaaa
     5401 agtatttgaa cgtttctgga aggcgaaaac cacggagaac aatttttatt caaatggtgc
     5461 cggaatcggg ctctccctgt caaaagagct ggtagagctt caccacggtg aaatatttct
     5521 gcaaagtcac cccggcgcgg gaagcacttt tacctttaca ttgcgtttgg gcagtaaaca
     5581 t
//
LOCUS       DAOSDR010000214         5563 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrimonas sp. isolate AMR_MDS_1826
            NODE_5221_length_5563_cov_3.694626, whole genome shotgun sequence.
ACCESSION   DAOSDR010000214 DAOSDR010000000
VERSION     DAOSDR010000214.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863355
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrimonas sp. (wastewater metagenome)
  ORGANISM  Petrimonas sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Dysgonomonadaceae; Petrimonas.
REFERENCE   1  (bases 1 to 5563)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5563)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:10:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,720
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 35
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5563
                     /organism="Petrimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5221_length_5563_cov_3.694626"
                     /isolate="AMR_MDS_1826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2023866"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            175..1017
                     /locus_tag="PLI30_08920"
     CDS             175..1017
                     /locus_tag="PLI30_08920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018110133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HOI79712.1"
                     /translation="MKTMDLLDEFERKMIVQRYSSNSIQNYKSAVRSFLQLAEKKYSH
                     PLEITETEIEKYIYWKIEKHRIGASYQRLIVASIDKFYLSMFNRQLNLNHLYPRSKNK
                     SLPVYLTAREVSRLMENVTNLKHKCIVQLLYGCGLRLNELLHLKLTDVDSKNKIILIR
                     KAKGGKDRVVMLSPLLLESLRHYYKIYQPEEYLIEGQGGGVYSEKSVQVIVKNAALKA
                     GITKKVTPHTLRHSFATHLLENGTDIRYIQELLGHKSVNTTEIYTHVTDIAKTKIKSP
                     LDLL"
     gene            complement(1057..3369)
                     /locus_tag="PLI30_08925"
     CDS             complement(1057..3369)
                     /locus_tag="PLI30_08925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019539734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HOI79713.1"
                     /translation="MNQKILFSLFLCGILLPLRAQIKGTVTDSDSNPIPFANVAIYSL
                     PDSTLITGTTTDQQGVFSLLADNNSSNALLRVSFIGYETQTVPALPEQTISLQPDTTL
                     LDEVVVEGDLPRIRLRSDAVVATVQNTVLSKAGTANDVLKRLPSITGDNGDFSIFGKG
                     KAKIYVNNRELRDVSELDLIASADIREVEIVHNPGAGYDASVKAVIRIHTVRKTGDGL
                     SFDIRSTYLQTENTDLRQQLNLNYRHKGWDIFGTVKYERYAYVQDSRIAQEAFVDTLW
                     TQKNELRVDGLSNPLTTIAGVNYEISSNHYAGVKYTMTAFPGKNGMQSTTLSDVYADG
                     VFYDRWESNDNQTQNSKPRHRLNAYYNGKFGELNVDFNSDFYRSSQSTASSITESSQE
                     YDDRVITSDNRIKNRLFATRLVLSYPVWEGQLSAGNEFTRTRRDDDYTTDIQGIPSTN
                     TSIHDENNAFFAEYSRSTPIGQLTGGVRFEKVYSDYFSDGEKMDEQSRHYAHWFPNFS
                     YSNTLGGVQLQLSYTAKTVRPAYWQLGSSIFYANRFTMQTGNPFLKPTIIHDASLTGS
                     WKYLQLMVSYKQERDVIMQWATQMEENPAVTLLSTRNLDKLPSLTAYLTASPKFGIWA
                     PQASIGFMKQWLTITSNDKQVRLNSPIPTASLNNSFSLPKGFLLTLDANFQGKGNQQN
                     VELTDHQFVVNLGVTKSFFDDRLSVVLKGHYLFHGRTMDIKAYNDRLNIYQFSRWDTR
                     ELELTVRYKFNTAKNRYKGTGAGQGEINRM"
     gene            3543..4199
                     /locus_tag="PLI30_08930"
     CDS             3543..4199
                     /locus_tag="PLI30_08930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI79714.1"
                     /translation="MQIKYLTLFLLLSTTVCLKAQETVTLSHYLFPDFQPGVVLLKNG
                     VRNNAKLNYNAASEEMVFDENGKTLAIGDAVLPQIDTVFVADEKFIRINNKFVRILID
                     KPEIDLFVEHKCRLIPPGKPAAYGGTSQTSSTTSYSSILSDGRVYDLKLPDDYKIIPY
                     NVFWLNRNGKLQSFSNLGQLKKIYRDKKKLVGDYLKKNEVKLANEKSVEEAITYLENN
                     "
     gene            4216..5517
                     /locus_tag="PLI30_08935"
     CDS             4216..5517
                     /locus_tag="PLI30_08935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008769537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-fucosidase"
                     /protein_id="HOI79715.1"
                     /translation="MKRLIILSLLAVAIAGSSQAQQKYVPAAENLKNRAEFQDMKFGV
                     FIHWGIYSMMADGEWIMNNKNINWQEYAKLADGFYPSKYDAEKWVADVKAAGAKYICI
                     TSRHHDGFSMFHTRQSPYNIVDGTPFKRDVIKELADACHKQGVKLHFYYSQLDWRRSD
                     YFPIGRTGRGVGRTEQGNWDSYHRFMLNQLTELLTNYGEIGAIWFDGQWDQPADFNWR
                     LREIYDHIHSIQPGCLVISNHHLAPQEGEDGQTFEKDLPGHNTTGFSANAEIGQLPLE
                     TCETMNNSWGYNITDLKYKSEKELIHYLVKAAGNNANLLMNVGPRPDGTFPDIAVQRY
                     KAMGDWLKVYGESIYGTRGGLVTPRDWGVTTQKENRLYVHILNYKDKSLYLPTNGKKV
                     KSARVFLDKKPVKFTQDADGVLLKLDKVPDELDYVVELELK"
BASE COUNT         1442 a         1260 c         1447 g         1414 t
ORIGIN      
        1 tgttgtttac gcaataaata tacaactttc gtaaataccc gatttataac attcgcttat
       61 atatattaac attaaaatat tttgcattta tatcatattg tatttgtgtt tgttatgttt
      121 taattaattt ttttatattt ttgcatatac gaatgtttca caaaatcgaa gacaatgaaa
      181 acaatggatt tattggacga gtttgaacga aaaatgattg ttcaacgata cagttccaac
      241 tccattcaaa attacaagag tgcggtgagg agttttttac agctggctga aaagaaatac
      301 agccatccgc tagagataac ggaaacggaa attgagaaat atatatactg gaaaattgag
      361 aaacatcgca tcggagcatc ttatcaacgt ttgatcgtag cttcaataga caagttttat
      421 ctttccatgt tcaacagaca actcaaccta aaccatctgt atccccgctc gaagaacaaa
      481 agtctgccgg tttacctgac tgcccgggag gtgagccggt taatggagaa tgtgaccaac
      541 ctgaagcata aatgtatcgt acagctgtta tatggatgcg gactgcggtt aaacgaactg
      601 cttcacctga aactgaccga tgtcgattcg aaaaataaaa ttatcctgat tagaaaagcc
      661 aaaggaggaa aagaccgtgt ggtgatgctt tcccctctgt tactggaatc tttaaggcat
      721 tactataaga tatatcaacc ggaggaatac ctgatagagg ggcagggagg gggtgtctat
      781 tccgaaaaga gcgtgcaggt cattgtcaag aatgcagctt taaaagcggg catcaccaaa
      841 aaagtgactc cccatacctt gcgccacagc tttgctaccc acctgctgga aaacggtacg
      901 gatatccggt atatccagga attgctcgga cacaaatcgg tgaatacgac cgaaatttat
      961 acccatgtta ccgacatcgc gaaaaccaaa ataaaaagcc cgctcgatct gctttgatgc
     1021 agggcggcag gctttatttt gagtgaaaca gtacttttac atccggttga tctctccctg
     1081 gccggcgccg gttcccttgt accggttctt ggcggtattg aatttgtagc gcaccgtcag
     1141 ctccagctcg cgcgtatccc agcggctgaa ctggtaaatg ttgaggcggt cgttataagc
     1201 tttgatatcc atcgtacgcc cgtggaagag atagtgccct ttcagcacta ccgacagccg
     1261 gtcgtcgaag aacgatttgg tgactcccag gttgaccacg aactggtgat cggtcaattc
     1321 cacgttttgc tggttcccct tgccctgaaa attggcgtcg agggtcagga ggaatccttt
     1381 cggcaacgaa aaggaattgt tcagcgaggc ggtgggtatg gggctgttca ggcgtacctg
     1441 cttatcgttg ctggtgatgg tgagccattg tttcataaac ccgatggagg cctgcggagc
     1501 ccagatgccg aatttgggag aagcggtgag gtaggcggtc aggctgggca gcttgtccag
     1561 gttgcgcgta gaaaggaggg ttacggcggg attctcttcc atctgtgtcg cccactgcat
     1621 gatcacatct ctttcctgct tgtagctgac catgagctga aggtatttcc aggaaccggt
     1681 gagcgaagca tcgtggatga tggtcggctt gaggaaaggg ttgcctgtct gcatggtgaa
     1741 ccggttggca tagaagatgc tgcttcccag ctgccagtat gcgggacgga cggtctttgc
     1801 agtatagctt agttgcagtt gcactccgcc cagtgtattg ctgtaggaga aattgggaaa
     1861 ccagtgcgcg taatgccgac tttgttcatc catcttttcg ccgtcgctga aatagtcgga
     1921 atagactttc tcaaagcgga ctccgccagt taactgtccg ataggggtgg aacgggaata
     1981 ttcagcgaag aaagcgttgt tttcgtcgtg gatggaagtg ttggtggaag ggattccctg
     2041 gatgtcggtg gtgtagtcat cgtcccggcg ggtacgggta aactcattcc ctgccgagag
     2101 ctggccttcc caaacagggt aggagagtac cagcctggtg gcaaacaggc gattcttgat
     2161 ccggttgtcg gaggtgatga cccggtcgtc atactcctgg ctgctctcgg taatggagga
     2221 ggcggtcgat tggctgctcc tgtagaaatc gctgttgaaa tccacgttca gttctccgaa
     2281 cttgccgttg taataggcgt tcagcctgtg cctgggtttg ctgttctgtg tctggttatc
     2341 gttgctttcc caccggtcgt aaaacactcc gtcggcatag acgtcgctca gcgtggtgga
     2401 ctgcatgccg ttttttcccg gaaaggcggt catggtgtat tttactcccg cataatggtt
     2461 ggatgaaatc tcgtagttga ctcccgcaat ggtggtcagc ggattggata gcccgtccac
     2521 acggagttcg tttttttgtg tccaaagggt atctacgaag gcctcctggg ctatccggct
     2581 gtcctggacg taggcatacc gttcatactt gaccgtcccg aagatatccc atcccttgtg
     2641 gcggtagttc aggttgagct gctgccgcag gtcggtattt tccgtttgca ggtaggtaga
     2701 ccgtatatcg aagctcaatc cgtcgccggt cttgcggacg gtatgaatac ggatcaccgc
     2761 ctttacggag gcatcgtatc ccgcgccggg gttgtgcaca atctccactt ccctgatgtc
     2821 ggcggacgcg atcaggtcga gttccgacac gtcgcgcaac tcacggttgt tgacgtaaat
     2881 ctttgccttc cctttgccga agatcgagaa atcgccgttg tcacccgtga tggagggcaa
     2941 ccgtttcagc acatcgttgg ctgttccggc cttgctcaat acggtgtttt gtacggtagc
     3001 caccacggca tcactgcgca gcctgatgcg gggcaggtct ccttctacta ccacttcatc
     3061 cagcagggta gtgtccggtt gaagggagat cgtttgttcg gggagcgccg gcaccgtttg
     3121 tgtttcgtat ccaataaacg atacccgcag gagtgcattg gagctgttgt tatccgcaag
     3181 aagcgagaag acaccttgtt gatcggtggt ggttcccgtg atcagtgttg agtcggggag
     3241 ggagtagatg gccacattgg caaatggaat gggattgctg tcgctatcgg ttaccgttcc
     3301 ttttatctgg gcacgcaggg gcaacagtat gccacataga aataacgaaa agagaatttt
     3361 ttgattcatt gtgtttttgt tttaatccga taataggaca accgtttcct ttgtttggtt
     3421 acaacctgta caattttttt tgccggaaaa agaatatcca ttatctttac gtcgtccaat
     3481 cgcatcaaaa aggtacggtg caatgaaacc ggcacaatta aagtaatcgt tggacaggca
     3541 acatgcaaat caaatacctg actttattcc tcctgttgtc gacaaccgtt tgcctgaaag
     3601 cacaggaaac ggtaaccctg tcgcattacc tgttccccga tttccagccg ggggtggtgt
     3661 tgctgaaaaa cggcgtgagg aacaacgcca agctcaatta taatgcagca tcggaagaga
     3721 tggtttttga tgaaaacggc aaaaccctgg caataggaga tgccgtcctt ccccagatag
     3781 ataccgtgtt cgttgcggat gagaagttta tccggataaa caataaattc gtaagaatcc
     3841 tgatcgataa acctgaaatt gatttgtttg tggaacacaa atgcaggctg atccctcccg
     3901 ggaaaccggc cgcttacgga ggaacgtcgc aaacatcatc cacaacatct tattcttcca
     3961 tcctgtcgga cggcagggta tatgacctga aacttcctga cgactacaag attatacctt
     4021 acaatgtgtt ttggctgaac cggaacggga agttgcaaag tttttcgaac ctcgggcagt
     4081 tgaagaaaat ttatcgggac aaaaagaagc tggttggcga ttacctgaaa aagaacgagg
     4141 tgaagctggc taatgaaaag agtgttgagg aggccataac ctatctggaa aacaattaaa
     4201 tatatacgat taacaatgaa gcgactaatt atcttatcgt tgctggcagt tgctatagcc
     4261 ggcagttccc aggcacaaca aaaatatgtg cctgcagcag agaacttgaa aaacagagcc
     4321 gagttccagg acatgaaatt cggggtattc atccattggg gtatttacag catgatggcc
     4381 gacggggagt ggatcatgaa caacaagaac atcaactggc aggagtatgc caagctggcc
     4441 gatgggttct atccttctaa atacgatgct gaaaaatggg tggcagatgt gaaggcggcc
     4501 ggagcaaagt acatttgcat cacttcacgc catcacgatg gcttttcgat gtttcatacc
     4561 cggcaatcgc cttataacat tgtggacggc acaccgttca agcgcgacgt catcaaagag
     4621 ctcgcggatg cttgccacaa gcagggagtc aaactgcatt tctattactc ccaactcgac
     4681 tggaggcgat ccgactattt tccgatcgga cgcacgggcc gtggagtagg acgcaccgaa
     4741 cagggaaact gggactccta ccaccggttc atgctcaatc agcttaccga actgttgacc
     4801 aactacggag agatcggcgc aatatggttt gacgggcaat gggaccagcc tgccgatttc
     4861 aactggcgac tgcgtgaaat ctacgaccac atccacagca tacagccggg atgccttgtc
     4921 atcagcaatc accaccttgc cccgcaggaa ggggaggacg gacagacttt cgaaaaagac
     4981 cttccgggac acaacacaac cggattctcg gccaacgcgg aaattggaca acttccgctc
     5041 gaaacgtgcg aaacgatgaa caattcgtgg ggatacaaca ttaccgacct gaaatacaaa
     5101 tcggagaagg aactgattca ttatttggtt aaagcggccg gcaataatgc caatctattg
     5161 atgaacgttg gaccgcgtcc agacggtaca ttccccgata tagctgtcca gcgctacaaa
     5221 gccatggggg attggttgaa ggtgtacggt gaaagcattt acggtacacg gggagggctt
     5281 gtcacaccgc gcgactgggg tgtcaccacg caaaaagaga acaggctcta cgtccatatc
     5341 ctcaactaca aagataaatc gctttatttg ccgacgaacg gtaaaaaagt gaagtcggcc
     5401 cgcgttttcc ttgataagaa accggtgaaa ttcacgcagg acgctgacgg tgttctgttg
     5461 aaactcgaca aggttcccga tgaactggat tacgtggtag aacttgagtt gaagtaagag
     5521 tttagttttt tattttgaac gtgaacgtct tttgagagaa ata
//
LOCUS       DAOSDR010000215         5559 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrimonas sp. isolate AMR_MDS_1826
            NODE_5224_length_5559_cov_4.194222, whole genome shotgun sequence.
ACCESSION   DAOSDR010000215 DAOSDR010000000
VERSION     DAOSDR010000215.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863355
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrimonas sp. (wastewater metagenome)
  ORGANISM  Petrimonas sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Dysgonomonadaceae; Petrimonas.
REFERENCE   1  (bases 1 to 5559)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5559)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:10:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,720
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 35
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5559
                     /organism="Petrimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5224_length_5559_cov_4.194222"
                     /isolate="AMR_MDS_1826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2023866"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..410
                     /locus_tag="PLI30_08940"
     CDS             <1..410
                     /locus_tag="PLI30_08940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019538602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HOI79716.1"
                     /translation="EIEVIWGAAVEKDLEEEVKVTLLATGFSIMDVPGIEEQHKAQTK
                     AEELERKIKEDQRRQEEEDEQKLIDKYYGTLGLKMLSTANYKFEPFILTMDELDDDKV
                     LEALEKTPVFKRDRDFNPRLFKTETRSATSLFD"
     gene            476..925
                     /locus_tag="PLI30_08945"
     CDS             476..925
                     /locus_tag="PLI30_08945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005942510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HOI79717.1"
                     /translation="MNLFDTISDEIKKAMLARDKVRLEALRGVKKEFLEAKTAKGASD
                     ELSDEAATAILQKMVKQRKDSAEIYTAQGRADLASDELAQLRVIQEFLPQQLSPEELE
                     STVKSIISETGAQSMKEMGKVMGVATRQLAGKAEGRAISEMVKKLLS"
     gene            948..1823
                     /locus_tag="PLI30_08950"
     CDS             948..1823
                     /locus_tag="PLI30_08950"
                     /inference="COORDINATES: protein motif:HMM:NF014670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="creatininase family protein"
                     /protein_id="HOI79718.1"
                     /translation="MKYLLPLVALLIVFSIQAQSLSIKMEELSAPEFISAVEKSSKTI
                     ILPIGVFEKHGPHMPVGTDLYTAREIALRAAEKEYTVVFPWYYFSQINEARHQPGTIA
                     YSPEIIWKVLQETLNELSRNGFKKIIIINGHGGNNAFLNYFGMAQLSEKRDYALYWFQ
                     PDYDPEVIKKAEALTQHDRYDQHAGNRETSMVAAIVPDLVHPEKAGLQSGVDLDRLKN
                     LPSVYSGIWWYARYPNHYSGDGRKANAHAGELILNSVVEQFVKSIQDIKADKNVPELQ
                     NQFFEEAENPLDTKQ"
     gene            complement(1936..2151)
                     /locus_tag="PLI30_08955"
     CDS             complement(1936..2151)
                     /locus_tag="PLI30_08955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI79719.1"
                     /translation="MYAFLNQVNSQKSFIKSEKYIVLDANTVLYTATSRWETKMKNDS
                     TIVMDPLGMQFLLKKVDNQWRVLSWTE"
     gene            complement(2345..3897)
                     /locus_tag="PLI30_08960"
                     /pseudo
     CDS             complement(2345..3897)
                     /locus_tag="PLI30_08960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004318216.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
     gene            complement(3918..4859)
                     /locus_tag="PLI30_08965"
     CDS             complement(3918..4859)
                     /locus_tag="PLI30_08965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005675936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HOI79720.1"
                     /translation="MDRRNFLKAVALTGAAVTTIKDADAMSVLTQSFQTTNAAAKYDM
                     VAVLGGEPEAMFRKAIAELGGMKNFISKGDRVCVKPNIGWDKTPELAANTNPKLVTEI
                     IKQCFDAGAREVTVFDHTCDDWRKCYANSGIEAAAKAAGAKVVPAHQESYYKSVSLPK
                     GRSLKEAKIHQAIVDSDKWINVPILKNHGGAQLTISMKNYMGIVWDRGFFHANDLQQC
                     IADVCTYAKRPVLNVVDAYRLMKTSGPRGKSDADVVLSKGLFISQDIVAVDTAAANFF
                     NQAREMPLDKVGHLAKGEQLGVGTMNLDKLNIRRVRI"
     gene            complement(4892..>5559)
                     /locus_tag="PLI30_08970"
     CDS             complement(4892..>5559)
                     /locus_tag="PLI30_08970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019541078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOI79721.1"
                     /translation="WIPQFGLSFTPSASTVVKAIVSKGFRNPTLREMYMFPPQNPDLL
                     PERLMNYEISFMQSLLENRLNMGLNLFYIKGDNMIQQAEPGIGKWGNTGKVENKGFEI
                     STHYQVARDFRLSANYSLLSMAYKILAAPEHKLYVSANYTKNRWNLSTGIQYVGNLYK
                     TVKPEPVKENFVLWNARINYRALDWLNLFLKGENLLGQEYEINAGYPMPKTTAFGGIQ
                     LHF"
BASE COUNT         1614 a         1170 c         1215 g         1560 t
ORIGIN      
        1 acgagatcga ggttatttgg ggggctgccg tggaaaagga tttggaggaa gaggtcaaag
       61 taacattact agcaaccggt ttttccatta tggatgttcc cggaattgaa gagcagcaca
      121 aagcgcaaac gaaagcggaa gagttggaaa gaaaaattaa agaagatcaa cgaaggcaag
      181 aggaggagga tgaacagaaa ctcatcgata agtattacgg gacattggga ttgaaaatgc
      241 tttctaccgc caattataaa tttgaaccct ttatccttac tatggacgaa ctcgacgatg
      301 ataaggtgtt ggaggcactt gagaaaacac cggtatttaa gcgcgaccgg gatttcaatc
      361 ctcgattatt caagacagaa acccgctcag caacatcgtt gtttgattag cggagagcac
      421 aaggcaagcc tcgacgttga accttgaact ttaaacattt aaatagttaa acattatgaa
      481 cctgttcgat acaattagcg acgaaataaa aaaagcaatg ctcgccagag ataaagtgcg
      541 tttggaagca ctgaggggcg tgaaaaaaga atttttagaa gccaaaacgg ctaaaggtgc
      601 atctgatgag ctttcggacg aagcagcaac ggcaattctg caaaaaatgg tgaaacaacg
      661 caaagacagt gcggaaattt ataccgcaca gggaagagca gacctggctt ctgacgaatt
      721 ggcgcagttg cgtgttattc aggaatttct tcctcagcaa ctttcaccgg aagagttgga
      781 gtcgaccgtg aagtcgatca tttctgagac cggtgcccaa tccatgaagg agatgggaaa
      841 ggtgatggga gtcgctaccc gtcagctggc aggaaaggca gaaggacggg ccatttcaga
      901 aatggtgaag aagttattaa gttgaaataa tgcctaaacc tattcctatg aaataccttt
      961 taccactggt tgcattgttg attgttttca gcatacaagc acagagtttg tctattaaaa
     1021 tggaagagct ttcggctcca gaatttattt cagcagttga aaaaagctcc aaaaccatta
     1081 ttttacccat cggcgttttt gaaaaacacg gtccacatat gccggtcgga acagatttgt
     1141 atacggcgag agaaattgcg ctccgagcag ccgagaaaga atacacggta gtttttccat
     1201 ggtattattt cagccagata aacgaagcgc gtcatcagcc gggaacgatt gcttattcac
     1261 ccgagattat ctggaaagta cttcaggaaa cactcaatga acttagtcgg aacggtttta
     1321 agaaaataat cattataaac ggacatggcg ggaacaacgc gtttctgaat tattttggta
     1381 tggcgcaatt atccgaaaaa agagattatg cactttactg gtttcaaccg gactacgacc
     1441 ctgaagtgat caaaaaggcg gaagccctga ctcaacacga tcgttatgat caacacgctg
     1501 gaaacaggga aacctctatg gtggcggcaa ttgttccgga cttggttcat cccgaaaagg
     1561 cagggctaca atcgggtgtg gatttggaca ggttgaaaaa tcttcccagt gtttattccg
     1621 gaatatggtg gtacgcccgg tatcctaatc attattctgg agatggaagg aaggccaatg
     1681 cccatgcggg agaacttatc ttaaacagtg tagtggagca atttgtaaaa agtatccagg
     1741 atatcaaggc ggataaaaat gtgcctgaat tgcaaaatca gttttttgaa gaagccgaaa
     1801 atccgttgga tacgaaacaa taaaatattc acggggctac acttttagtc atcttatgag
     1861 tatttactaa cggagtaact tgtcctatcc actgacacgg atggcgtgtt gaaatacaac
     1921 tccattacct taaaactact ctgtccaact cagtactctc cactgattat caactttttt
     1981 caatagaaat tgcatgccca acggatccat aacgatggta ctgtcgtttt tcattttagt
     2041 ttcccatctc gaagttgctg tatataaaac agtatttgca tccagcacta tatacttttc
     2101 actttttatg aatgatttct gactgtttac ctggttcaga aaagcataca tatttttaac
     2161 agactgatcg taatctgcat aaacgcctcc aaccaaagaa acaaaatcag gtgagtccag
     2221 gtatattgaa atatactcaa cttttgttat cgttcaataa aattgataat atttttcaaa
     2281 atcaaaaaca aagcgccctt gcgggcgcct gataaataaa tttcttatac agagattcta
     2341 cttcttaaaa tccaaactca tcaatggtca cttcttccac ttcttcttgt ttaatctcaa
     2401 tggtgttgtg agcagtaagt ccttccacgt aaatggcttt ataaggaatt gccgggcaca
     2461 catactcaca tccgccacaa cctacacaaa tttcgggttc gatttcgggg attgttaacg
     2521 cacctttgta aggaaccatt cgaacggctt gtgtagggca gtgttcggag caggcgccac
     2581 agctggtttc atcgtaataa acgatacagt tctctataat gaagttaact cttcccatct
     2641 gtgtgtggtg cttttcctcc tgactaagct ttaccagtgc atctgtcgga caaacgtcgc
     2701 agcaaacagt gcaatcgtag ttgcaaaagc cgtgatcgaa ataaagtttc ggttgcatca
     2761 ccccgcccag tccatattcc agaaacgccg gttttatcac gtgcgacgga catttgctca
     2821 cgcaaagatg acacgaaata catttctccc ggaaatgatc gaagtttagc gctcccgggg
     2881 gagcaatcgg agtttttcgt ttcagttcgc gtttaggtaa taacggttct gttactgttt
     2941 gagcaagtag ttgacctgct gctatacttg tgattagtgt agctgacagg aaacgacgct
     3001 tcgattcgtc tttcggctta tcttccctaa tgtatgaaaa agccggagcc gtttcaactg
     3061 cctttttttt cttggagcca ataagagcat atttcatcgc atcacgatgg caaacttcaa
     3121 tacagttaaa gcaggttacg catcggttat gatcgatgac tttatttttt gcatcaatac
     3181 acgatgcttt gcatttcatc gaacacaaac cgcacatgtt gcacttgtca tctataaatt
     3241 gaattttgaa aagtgaaaac cggctcagaa atccaagtgt agttcctact ggacaaattg
     3301 tattgcaata tgtccttccg tttttccacg ctaaaaaacc gataacaagc agcgtaatta
     3361 aagcgataat tatcgaagat actcccaacg aatagatacc gactttataa aaactgtaat
     3421 tattgaatga tgagaaaata gcttccagga aattatttcc tgccagataa gcaggacgga
     3481 aaatgtgtgt taccaatctg ccgtatgcac cgtacggatc gagcaggcct accaagaagg
     3541 tgaatccaaa caagaaagca ataattgtag cacccaagac actccatcgg agaacgattt
     3601 ttgctttgct gtaggtgtat cttttcttct tcttaagtat ttttttgaaa accaggcaac
     3661 aatgtcctga tagataccca ttgggcagat agatgaacaa tacacccggc cgaaaattac
     3721 tgtaagcgct actaatgcaa tcagaataat aatattaagt gctaataatg caggaataaa
     3781 ttgtatatcg gttagaacgc tgaaactttc cggcagaaaa cctgaaaagt cgagaaagta
     3841 gagcgttatt aaagaaaaaa gtagtacaga tatcgttacc cggatttttt taagcatgat
     3901 tgttcgatcg tgtttggtta aattctcacc ctcctgatgt tgagcttgtc gagattcatc
     3961 gtccccacac ccaattgctc cccttttgcc aaatggccaa ccttatccag cggcatttcc
     4021 ctggcctggt tgaagaaatt ggcagcagca gtgtcaacag caacgatatc ctgcgagatg
     4081 aagaggcctt tcgacaatac tacatcggca tccgattttc ctctggggcc gctagtcttc
     4141 atcaaccggt aggcatcaac aacattcagg accggacgtt ttgcataggt acacacatca
     4201 gcgatgcatt gctgcaaatc gttggcgtgg aaaaatcccc tgtcccatac aattcccatg
     4261 tagttcttca tggagatggt caattgtgca ccgccgtggt ttttgagaat aggaacattg
     4321 atccatttgt cggagtcgac aatggcttgg tgtattttgg cctctttcag gcttctaccc
     4381 tttggcaaag agacactctt gtaataggat tcctgatgtg cgggaacaac ttttgccccg
     4441 gcagctttag cggcagcttc gattccgctg ttagcgtaac atttacgcca gtcgtcgcac
     4501 gtgtggtcga aaacagttac ctcacgggca cctgcatcga aacattgttt gatgatttcc
     4561 gtcaccagtt ttggattggt gttagcggcc agttcgggag ttttatccca tccgatattc
     4621 ggttttacac aaaccctatc tcccttgctg atgaagtttt tcattccccc caattcggca
     4681 atagctttgc gaaacatagc ttcgggttcc ccgcccaata cggcaaccat gtcgtatttt
     4741 gctgcagcat ttgtagtttg aaacgattga gtgagtacgc tcatcgcatc tgcatcttta
     4801 atggttgtga cagctgcgcc ggtaagagca acagctttca agaaatttcg tctgtccata
     4861 atgtttttaa tttgttgttc gttctgtaat ttcaaaaatg taactggatt ccgccgaagg
     4921 cagtagtttt cggcatcggg tatccggcat ttatttcgta ttcctgtccc aataaattct
     4981 caccttttaa gaacaggttt aaccagtcaa gtgcccggta attgatgcgg gcgttccaaa
     5041 ggacaaagtt ctcctttacc ggctctggct ttacggtttt atacaagttt ccgacgtatt
     5101 ggataccggt agataaattc cacctgttct tcgtgtaatt tgcactgacg tatagtttgt
     5161 gttccggagc tgccaatatt ttataggcca tgctcaataa actgtaattg gccgaaagcc
     5221 gaaaatcacg ggcgacctga tagtgggtgc tgatctcgaa gcctttgttt tctactttac
     5281 cggtatttcc ccatttgcca atcccgggtt cggcttgttg aatcatattg tcgcctttga
     5341 tataaaagag gtttaaaccc atattcaacc ggttttcgag caatgactgc atgaacgaga
     5401 tttcgtaatt catcaatctt tccggaagca gatcggggtt ttgcggaggg aacatataca
     5461 tctcgcgaag ggtagggttt cggaatcctt tgctgacgat ggctttcaca acggttgagg
     5521 cggagggggt gaaacttaat ccgaattgag gaatccatt
//
LOCUS       DAOSDR010000216         5554 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrimonas sp. isolate AMR_MDS_1826
            NODE_5232_length_5554_cov_3.851064, whole genome shotgun sequence.
ACCESSION   DAOSDR010000216 DAOSDR010000000
VERSION     DAOSDR010000216.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863355
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrimonas sp. (wastewater metagenome)
  ORGANISM  Petrimonas sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Dysgonomonadaceae; Petrimonas.
REFERENCE   1  (bases 1 to 5554)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5554)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:10:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,720
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 35
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5554
                     /organism="Petrimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5232_length_5554_cov_3.851064"
                     /isolate="AMR_MDS_1826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2023866"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..567)
                     /locus_tag="PLI30_08975"
     CDS             complement(<1..567)
                     /locus_tag="PLI30_08975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018965510.1"
                     /note="catalyzes the formation of
                     2-methylthio-N6-(dimethylallyl)adenosine (ms(2)i(6)A) at
                     position 37 in tRNAs that read codons beginning with
                     uridine from N6-(dimethylallyl)adenosine (i(6)A); Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HOI79722.1"
                     /translation="MSTETTDNQKEKRLFIETYGCQMNVADSEVVAAIMEMDGYSVTE
                     DEKAADAIFVNTCSIRDNAEQRVIQRLDYFNALKKKKRGELIIGVLGCMAERVKEELI
                     DNHHADLVIGPDAYLDLPNLMGAAERGEKAINVVLSKTETYKDVIPLKMSGLNISGFV
                     SITRGCDKFCHYCIVPFTRGRERSREPES"
     gene            1058..1297
                     /locus_tag="PLI30_08980"
     CDS             1058..1297
                     /locus_tag="PLI30_08980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI79723.1"
                     /translation="MASARSTRWEFQSSLDLVTDFEGESSLKGSMFATERFDNHHEMK
                     EMETVHPILEQLRSKNGQSEIRKAVIYSEILNRKY"
     gene            1350..2852
                     /locus_tag="PLI30_08985"
     CDS             1350..2852
                     /locus_tag="PLI30_08985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005634657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008775 - acetate CoA-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0006084 - acetyl-CoA metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate CoA transferase"
                     /protein_id="HOI79724.1"
                     /translation="MSLTYVTAKEAAALVNHNDNVGFSGFTHAGCPKVVPVEIAKRAE
                     EEHAKGNPFKIGMFTGASTGDSIDGSLARANAVKFRTPYQTNKDMRNAINAVQHEPVQ
                     YFDMHLSQVAQEIRYGFLGGVDVAIVEACEVTDSGEIVPTSGVGIMPTICRLAKIVIV
                     ELNDKVPAKMRGVHDLYELQDPPKRRQINIFEVQGRVGLEYVKVDPSKIYVVRTSKEN
                     EGSGFAPVDEITEKIGENVSNFFVSELKKGHIPPTFLPIQSGVGNIANAVLASMAQNK
                     DIPRFEVYTEVIQDAVLDMMQKGHISFASGCSLTLSNEAMERFYRDLDFFKNKLVLRP
                     SEISNNPGLVRRLGVIGINTAIEADIFGNVNSTHVMGNMMMNGIGGSGDFTRSAYISI
                     FVTPSTAKDGKISAIVPKVAHEDHSEHSVKVIVSEYGVADLRGKGTYARAEEIIENCA
                     HPSYRPLLHDYLSLTKKGHTPQNLYACFEFHKAFMETGDMINADFSKYKK"
     gene            2978..3229
                     /locus_tag="PLI30_08990"
     CDS             2978..3229
                     /locus_tag="PLI30_08990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018360313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HOI79725.1"
                     /translation="MGILSWIVLGLLAGLIAKAIRPGADPGGWIVTILIGIAGGVLGG
                     WIGSLLGWGTVDGFSLRTLLLAVGGALIILWVYALLKKK"
     gene            complement(3281..4696)
                     /locus_tag="PLI30_08995"
     CDS             complement(3281..4696)
                     /locus_tag="PLI30_08995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019540786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HOI79726.1"
                     /translation="MIESSFMLISAFVGEVYQEPSVYSLYLSSLITFVSGLVLFLIGR
                     KKRKEKRISQREVYLTVTLVWVMMALYGTLPFLIGGAIPSFTDAFFESVCGFTTTGSS
                     TLINIEAFPKSLHFWRSFTQWIGGIGIIIFVMSFLPLFGGSSSHLYNAEATGISKEQL
                     RLRVSDITRNMSFTYLGLTAVGFLLLWAGPMNAFDAACHAFTSISTGGFSTKQASIAH
                     FNSAYTEYVIIFLMFSGGIRFLLLFNLFRNFSGRIFKDEEFLWYSFMILGFTFIITMA
                     LYAEGNVLGSVEDTIRTVLFQVVAAITTTGYATTDFLRWGQFYWFLFLAMVLFCGSEG
                     STSGGMKISRLIILVKNTKVVFRRQVHPQALYMVKINGQVYSNAVVEKVLAFVFLYLT
                     ITGLGAIVLSFTGMSFDESIGAAVSSMSSYGFGLGDFGPSGNFSTATGFAKYFLSFLM
                     IVGRLEVFTVLSLFTSSLWKK"
     gene            complement(4750..>5554)
                     /locus_tag="PLI30_09000"
     CDS             complement(4750..>5554)
                     /locus_tag="PLI30_09000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019540787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NAD-binding protein"
                     /protein_id="HOI79727.1"
                     /translation="FHIVVIKRGNQTLIPKGSDQILPNDILYFTTLRKNLDEMPAIMG
                     KTSFETKNVMFMGGSRITMRAVQYLPSNINIKIIEQNRERAEKLVEISPSNVTIFLGD
                     ARDAELLLSEGISDMDAFVALTGNSEANILGCMMAKQYGVKKTIAEVENIDYIPMAER
                     FDIGTVINKKLLAASKIYELLLKADASNIKCLTFSNANVGEVVAKPNSKVTKKLIKNL
                     NLPSDMTLGALIRNGEPMLIDGDTLIEPYDQVMVFFMNTSLKSIESFFN"
BASE COUNT         1578 a         1149 c         1288 g         1539 t
ORIGIN      
        1 gctctccggt tcccggcttc gctccctgcc acgcgtaaaa ggaacgatgc agtagtggca
       61 gaacttatcg catccccgcg taatggaaac aaaccccgaa atatttaatc cactcatttt
      121 taatggaata acatctttat aggtttccgt tttggaaaga accacgttga ttgctttttc
      181 tccgcgttcg gcggccccca tcaggttggg taagtcgagg taggcgtcgg ggccgattac
      241 caggtcagca tgatgattat cgatcagttc ctcttttaca cgttctgcca tacatcctaa
      301 aacaccgatg atgagctctc ctctcttttt tttcttcaaa gcgttgaaat agtcaagccg
      361 ttgtatcacg cgttgttctg cattgtctcg gatggagcag gtgttgacaa aaatagcatc
      421 ggcagccttc tcgtcttcgg taaccgagta tccgtccatc tccatgatag ctgccactac
      481 ttcggagtcg gctacgttca tttggcaacc gtacgtttcg ataaatagcc ttttttcttt
      541 ttgattatct gttgtttctg tgctcatttt cctttttttg ctgtaatttt ttcttaatgt
      601 caggaattta taaacatctt agtatatttt aaactaaaat atttgtaaaa tttaaattct
      661 tgttttactt ttgtcacgaa cgaaaaaata aaaatttttt catagttaag gttttggtta
      721 aacaattagg ggcggctgtg aagttgcccc tgattttttg aataaccttt cctttattcg
      781 tgaaaattga ttacttttgt cggactgcaa aattaatcaa tttatggaat ttggcgacat
      841 tatttattta atactcatgc ttgctttctt tgtttttgga atttttaatg attcaaaaaa
      901 aaagaaaaag aaaataagta aaagtaccca acctgtaaat ttagaagaag agtttcgcga
      961 tgtgtttcgg gaagtttttc aaaaaccaca aagacaacct atttcacctc caactccaaa
     1021 gaccgttaag aatgaaaatc gactcgcaag gccagcaatg gcctcagcca ggagcactcg
     1081 ttgggaattt caatcttccc tggacctggt aaccgatttc gagggagaat cctcgttgaa
     1141 aggttccatg tttgcaaccg aacggttcga caatcatcat gagatgaaag aaatggaaac
     1201 ggtccatcct attctggagc aattgaggag caaaaacgga cagtctgaaa tacgcaaggc
     1261 ggttatctac agtgaaatac tgaacagaaa atactgacct gactcttgtg taactaaaaa
     1321 acaatgatat aaaaaacaaa caaaaatcta tgagtcttac ttatgtaaca gcgaaagagg
     1381 ctgctgctct tgtcaaccac aacgacaatg tgggttttag cggctttaca cacgccggat
     1441 gtcctaaagt ggttccggtt gaaattgcca aacgtgccga agaagagcac gctaaaggca
     1501 atccttttaa aataggcatg tttaccggtg cttccaccgg cgattctatc gatggtagcc
     1561 tggctcgcgc caatgcggta aaattccgca caccctatca gacgaataaa gatatgcgta
     1621 acgccattaa cgccgttcag catgaaccgg tacagtattt cgacatgcac ttgtctcaag
     1681 tggcgcagga aattcgttac ggttttttag gaggtgtgga tgtggctatt gtggaagcct
     1741 gtgaggttac cgattcggga gagattgttc ccaccagcgg tgttgggatt atgccaacca
     1801 tttgccgttt ggccaagata gttattgtgg agctgaacga taaggtaccc gccaaaatgc
     1861 gcggtgtgca tgatttatat gaattacagg atcctcctaa acgccgtcag ataaatattt
     1921 ttgaagtaca ggggcgtgta gggttggaat acgtgaaagt ggatccttca aaaatttatg
     1981 tggtaaggac aagtaaggaa aatgaaggca gcggttttgc accggttgat gagattacgg
     2041 aaaaaatcgg agagaacgtt tcgaactttt tcgtttctga attgaaaaaa ggacatattc
     2101 cgcccacttt cctgcctatt caatcgggtg tgggaaacat tgccaatgct gttcttgcat
     2161 ccatggcaca gaacaaggat attccccgct tcgaagtata taccgaggtt attcaggatg
     2221 ccgtgttgga tatgatgcaa aaagggcata tctcttttgc cagcggttgc tcgcttacgt
     2281 taagcaacga agcaatggaa cgtttttatc gcgatttgga ttttttcaag aacaagctgg
     2341 tgcttcgtcc gtcggaaatc tccaacaatc ccggtctggt gcgccggttg ggtgtaatag
     2401 gcatcaatac ggccattgaa gctgatatct tcggaaatgt aaactccacg catgtgatgg
     2461 gtaatatgat gatgaacggt atcggaggtt cgggtgattt tacgcgaagt gcttatatct
     2521 ctatttttgt tacaccttca accgcaaaag atggaaaaat aagcgccatt gttccgaaag
     2581 tggcacacga agaccacagc gaacattcgg tgaaagtaat tgtttcggag tatggcgttg
     2641 ctgatttgcg cggcaaggga acgtatgccc gtgctgaaga gatcattgag aattgcgcac
     2701 atcctagcta ccgcccgttg ttgcacgatt acctgagtct tacgaaaaaa gggcacacac
     2761 cgcagaattt atacgcttgt tttgaattcc acaaggcgtt tatggaaacc ggtgatatga
     2821 tcaacgccga cttttcgaaa tataaaaaat aatcgtggat gaatgtttct tccaatggtg
     2881 taggaacaga ggaaaatgat ggggcggttc gaacaaagcg ttaaaagaat acgttataga
     2941 atagtatttt ttaattagct aactaaaatt tttgattatg ggaattttat cttggatcgt
     3001 tttaggcctg ttagcaggtt tgatagcaaa agccattcgc cccggagcag atccgggcgg
     3061 ttggatcgta accatcttga tcggtatcgc cggcggtgtc ttgggcggat ggataggttc
     3121 gttgttaggc tgggggaccg ttgacggatt cagccttcgt acgctgttac tggccgttgg
     3181 aggcgcactg atcattctgt gggtgtatgc cttgttgaag aaaaaatgat gaacagttgt
     3241 tcaaggcaag gggcgacgca agttgccctt tgttgtttta tcacttcttc cataaacttg
     3301 aggtgaaaag cgagagtacc gtgaacactt caagccggcc tacaatcatc aggaaagaca
     3361 agaagtactt ggcaaaaccg gtagcggttg aaaagttccc cgacgggccg aaatcgccta
     3421 acccaaagcc ataactgctc atgctcgaaa ctgcggcacc aatggattcg tcgaacgaca
     3481 ttccggtaaa actcagtacg atggcaccca gtccggtgat agtcaggtaa agaaaaacga
     3541 aagcaaggac cttttcaact accgcatttg agtacacctg cccgttaatc tttaccatat
     3601 acaatgcttg cggatgaacc tggcgacgaa atactacctt tgtattcttt accaaaatga
     3661 ttaaccggga tatcttcatc ccgcccgatg ttgaaccttc gcttccgcaa aacagaacca
     3721 tagccaggaa aaggaaccaa taaaattgtc cccaacgcag gaagtccgta gtggcatatc
     3781 cggtagtggt gattgccgct acaacctgaa aaagaacggt gcggatagtg tcttctacag
     3841 atcccaacac gttaccttcg gcatacaagg ccatagtaat gataaacgta aagccgagaa
     3901 tcataaaaga gtaccacaaa aattcctcgt ccttgaaaat ccgacctgag aaattgcgga
     3961 acaggttgaa cagcaacaaa aaacggattc cgcccgagaa catcagaaat ataattacat
     4021 attccgtata ggcggagttg aaatgagcaa tgctggcttg tttggtggag aatcctccgg
     4081 tggagatgct ggtgaaagca tggcaggccg catcaaatgc attcatggga cctgcccaga
     4141 gcaggagaaa cccgactgct gttagaccca ggtaggtgaa ggacatgttt cgggtgatgt
     4201 cgctgacacg caggcggagt tgttctttgg agattccggt tgcctcggca ttgtaaagat
     4261 gggaggagct gcccccgaag aggggcaaaa aagacatcac aaaaatgatg attccgatgc
     4321 ctccaatcca ttgggtaaaa cttcgccaaa aatgaagtga tttagggaag gcttctatgt
     4381 tgatcagggt agagcttccg gtggtggtaa acccgcaaac gctttcaaag aaagcatccg
     4441 taaaagaagg gatggctcca ccgatcaaaa aaggcagcgt accgtaaagg gccatcatca
     4501 cccatacaag tgtgacggta aggtaaactt cccgttggga gatgcgtttc tctttacgct
     4561 tctttcttcc gattaaaaat aaaacaagcc ctgaaacgaa cgttattaac gatgacaggt
     4621 aaaggctgta aacggaaggt tcctgataaa cttcgccaac aaatgcggaa ataagcataa
     4681 atgatgattc gatcatcaaa ataagtccta tgctgctaaa tacgaattga tggttgaagt
     4741 ttcgcataat caattgaaga aactctcaat gcttttcaac gatgtattca taaaaaacac
     4801 cattacctgg tcgtatggct caatgagcgt atcaccgtca ataagcatgg gttccccgtt
     4861 tcggataagt gcgccgagag tcatgtcgga gggtaaattc aggtttttga tcagtttttt
     4921 ggtgaccttt gagttgggtt ttgcaacaac ttctcctacg tttgcatttg agaaagtgag
     4981 gcatttgatg tttgaagcat ccgctttgag aagaagttcg tatattttgc tggctgccaa
     5041 cagctttttg ttgattacgg tgccgatatc gaaacgttcg gccataggaa tataatctat
     5101 gttttctacc tcggcaatgg tttttttaac accgtattgc ttggccatca tacagcccag
     5161 gatgttggct tcggaatttc cggtaagcgc aacaaatgcg tccatatcgg agattccttc
     5221 actgagtaac aactctgcat cgcgcgcatc acccagaaat atggtaacgt tcgaaggaga
     5281 aatctctacg agtttttccg ccctttcccg gttttgttcg ataatcttta tgtttatgtt
     5341 tgacggcagg tattgcacgg cccgcatggt aatccggcta ccgcccataa acatgacgtt
     5401 tttggtttcg aacgatgtct tacccatgat agcaggcatt tcatcgaggt tttttcttaa
     5461 cgtggtaaaa tagagaatat cgttgggtaa gatctgatca cttcctttcg ggatcaacgt
     5521 ttggtttccg cgttttataa cgacgatatg aaac
//
LOCUS       DAOSDR010000217         5537 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrimonas sp. isolate AMR_MDS_1826
            NODE_5263_length_5537_cov_4.205217, whole genome shotgun sequence.
ACCESSION   DAOSDR010000217 DAOSDR010000000
VERSION     DAOSDR010000217.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863355
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrimonas sp. (wastewater metagenome)
  ORGANISM  Petrimonas sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Dysgonomonadaceae; Petrimonas.
REFERENCE   1  (bases 1 to 5537)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5537)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:10:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,720
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 35
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5537
                     /organism="Petrimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5263_length_5537_cov_4.205217"
                     /isolate="AMR_MDS_1826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2023866"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1403)
                     /locus_tag="PLI30_09005"
     CDS             complement(<1..1403)
                     /locus_tag="PLI30_09005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007654073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HOI79728.1"
                     /translation="MKHIISLFLLFVFFQFNSCTDVLDKAPDGNMQMNEVLADPDKVE
                     AMVNTLYANIPRKGYVYFFFDPAVVSCSDDGWSSEDGAGQLVDQYYKDNTSANNHPMR
                     DFPDGHGANNNAYWNRYWQQIRLCSQFLELIDKAAVNNETDRGRFRAEAHLLRAFFYM
                     ELVKWFGKLPVLDQTVPFDTDFSKMTRQSVYDAAKLIISDCNLALAEPNLPWRIDTET
                     NGGRVTKALACALKSEAILFAASPLHNEGKNYWEEAYQVTKEAVQQLKLNGYQLFTTC
                     TQPNIYGNGPAAAFRQLVTQNADYSGSPRDKETIFQTRSGSVFVWHIGYIGSNMANTY
                     KCGTCPTQELVDAFETINGQPVLDLTKPYLDEKHLVPNYNKANTLYNPNDPYANRDPR
                     LDQTVLRNGSHILWDNKDVTVQTYTGGAHAPSFDVTNRSASRTGYYHCKMVTPGACGT
                     NQINNANWRFYRLAETLL"
     gene            complement(1424..4867)
                     /locus_tag="PLI30_09010"
     CDS             complement(1424..4867)
                     /locus_tag="PLI30_09010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007654075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0071702 - organic substance transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SusC/RagA family TonB-linked outer membrane
                     protein"
                     /protein_id="HOI79729.1"
                     /translation="MKKNKFRVPRFRHFERLWLTMKICLFFLLITTATVSASVSYSQN
                     TKLTLNLQNTTIKQLIKEIEKQSEFIFVFYDDALDLNRRISIKAENLTVEKILEKVLE
                     TTGNTFAVFDRQIVIGKKNPETEVIQATIPAEQKKEITGKVLDKQNMPVPGTSVIIKG
                     TTFGVITDASGNFRLIYTGEAKVLVFSFVGMKTQEIQIGNKADFTVIMEEETVGLEEV
                     VAVGYGIQKKESVTAAISSVMTKDLKRSSAPNLSVALAGQLPGLTVMQSSGQPGNDVV
                     NLYLRGVSTLNDASPLILIDGIPRSNIDKLDPNEIASISILKDASATAVFGVRGANGV
                     ILITTRRGEEGKSKLSVTVDKSFQQFLVHNDRIHSWEFAELRNQAFLNGNPNATENEM
                     PFTQYMIDKYKSGEDPVFYPDRDVYHDYFRDWAPQTRVNANFNGGGKDFNYFLNAGYI
                     GQGGNFKTEPKSFLGYDPSYSMDRYNFRGNIDYKIAQNLKASLNIATYLQQMNSPQTV
                     DLFGGDVNGMVQNMMAYTWGTPPTDPGPVTVAGYGVPENEIIAQSGQDRNTYGEINRR
                     GYRQEYSTVLNSSLALDWGLGFITKGLSAKGMIAFDSHAATTRQGVRSLNTYAFYVAR
                     NATQKSGYSPIRDNQDKSIRLSKNMNTYYFMNYQVSLNYARSFGLHNVSGMFLHQRDN
                     WEGWSADLPYNVIGYVGRLTYDYDNRYLGEFNFGYNGSEQFAPKNRFGSFPAFSLGWV
                     ASNEGFLKGNPVITHLKFRASYGITGNDKLGNSRFLYQSFITMGGGEFPGLGLGQSVN
                     QGRMGNEGLQWEKAEKKNVGFDLKIVNNLSLTVDAFQEHRDKILISRSTIPVLQGVPI
                     SNIPKVNIGIVDNKGLETELAYLKVINPELSFSVKGNFTYNKNIVKFSDEVKYGEDYA
                     YPYRSTGFSIGQRFGYLIDYSNGNGYINTPEELANLPLYEVGGKPRLGDFKYVDVTQD
                     GKINNRDMVPIGNPTVPRISYGLSGTMKYKNFDMAFVFSGIAQSSVTHNGWGVTEFGL
                     VGFFSKWHFKAWTPERYANNEEILYPALGMSEGVSQKSNSFFIMDRSFLRLKNVEIGY
                     NMPKKILNPVGIENVRIYTNGNNLFTWDKLPVNTVDPEQNTTLVYPLTKMITVGVNMT
                     F"
     gene            complement(5041..>5537)
                     /locus_tag="PLI30_09015"
     CDS             complement(5041..>5537)
                     /locus_tag="PLI30_09015"
                     /inference="COORDINATES: protein motif:HMM:NF027664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4974 domain-containing protein"
                     /protein_id="HOI79730.1"
                     /translation="YLEVAHDPSKPFKVVTDKLQIEVLGTKFNVSAYPEDLSASVVLV
                     EGGIQATVDSKTMVIKPNQRLVYEKNSHAASLDDTDVLPYISWKDGWMYCEKESLESI
                     ATKLSRYYNVKIQFKDEESKKMSLTGKLDLKNECVEIFHAISSTAPITCEMQEDTIVI
                     TQQR"
BASE COUNT         1405 a         1223 c         1185 g         1724 t
ORIGIN      
        1 agcaaggttt cggctaaccg gtagaatctc cagttggcat tgtttatctg attggttccg
       61 caagctccgg gggtaaccat tttgcaatga taataaccgg tgcgtgacgc gctacggttg
      121 gtgacatcga aactgggagc atgtgctccg cctgtgtagg tttgaacagt cacatccttg
      181 ttgtcccaca ggatatggct tccgttacgc aaaacggttt ggtctaaacg cggatcgcga
      241 ttagcataag gatcattggg gttatacaaa gtatttgctt tgttgtagtt tggaacaaga
      301 tgtttctcgt ccagataggg tttggtcaga tcgagcaccg gttgaccatt aatggtctca
      361 aaggcatcca ccaattcctg ggtcggacac gtgccgcact tgtaagtatt ggccatattg
      421 ctgccaatgt acccgatgtg ccagacgaaa acactgcctg aacgggtctg aaagatggtt
      481 tctttatccc ggggtgatcc ggaataatcg gcattctggg taaccaactg acggaaagct
      541 gctgccgggc catttccgta aatattgggt tgggtgcagg ttgtaaatag ctgatagcca
      601 tttaatttta actgttggac tgcttctttg gtgacttgat aggcttcctc ccagtaattt
      661 ttaccttcgt tgtgtagcgg actggcggca aacaaaattg cttcggattt gagagcacag
      721 gccagggctt tggtaacacg tcctccgttt gtttccgtat caatcctcca gggtaaattc
      781 ggctcagcca gagcgagatt acaatcggag ataatcagct ttgccgcatc gtaaaccgat
      841 tgtcgggtca ttttggaaaa gtcagtatca aaaggaactg tttgatccag aactggaagt
      901 tttccgaacc atttaaccag ttccatgtaa aaaaatgccc gtaacaagtg agcttcggct
      961 ctaaatcttc cgcgatctgt ttcattgttt acagcagctt tatcgattaa ttccagaaat
     1021 tgtgaacaca agcgtatttg ttgccagtaa cggttccaat aagcattgtt gttcgccccg
     1081 tgtccatcag gaaaatctcg cataggatgg ttattagccg aagtattatc tttgtagtac
     1141 tgatcgacca attgccccgc accatcttcc gacgaccatc catcgtcact gcaggagacc
     1201 actgccggat caaaaaagaa atagacataa ccttttcttg gaatattggc atacaaagta
     1261 ttcaccatcg cttcaacctt gtctggatcg gccaatacct cgttcatttg catattaccg
     1321 tcgggagctt tgtccagaac atcggtacaa ctgttgaatt gaaaaaagac aaacaacagg
     1381 aatagagata taatatgttt cattttatat tcagatttaa cgtttagaaa gtcatgttaa
     1441 cccctaccgt gatcatttta gttaatggat atacaagggt tgtattttgc tccgggtcaa
     1501 cagtgttaac gggtaattta tcccaggtaa acaagttatt cccgtttgta tagatccgta
     1561 cattttctat tccaaccggg tttaggattt ttttaggcat gttgtaccca atttccacat
     1621 tctttaagcg caggaacgaa cggtccatga tgaaaaaact gttcgatttt tgactcacac
     1681 cttcagacat cccaagagct gggtacagaa tttcttcgtt gttggcataa cgttcggggg
     1741 tccaggcttt gaaatgccat ttgctaaaaa atccgaccaa accgaattca gtaactcccc
     1801 acccgttgtg ggtgacacta ctctgggcta tacccgagaa cacaaatgcc atatcgaaat
     1861 tcttgtattt cattgttcct gacaatccgt aggaaatgcg gggaacggtt ggattgccaa
     1921 tcggaaccat gtcgcggtta ttgatttttc cgtcctgggt aacgtcgaca tacttgaaat
     1981 cgccaagacg aggctttcct ccaacttcgt aaagtggcag attagccagt tcctccggcg
     2041 tattgatgta tccgttgcca ttgctataat caatgaggta tccgaaccgc tgaccaatac
     2101 taaacccggt acttcggtat ggataggcat aatcttctcc gtatttgact tcgtcgctga
     2161 actttacgat gtttttgttg taagtaaaat ttcctttgac actaaacgat aattctggat
     2221 tgatgacttt tagataggcc aactcggttt ccaatccttt gttatcaaca attccgatgt
     2281 ttacttttgg aatattgctg atgggcactc cctgaagtac cggaatggtg cttctgctga
     2341 tgagaatttt atcgcggtgt tcctgaaagg catccacggt aagcgacagg ttattcacga
     2401 ttttaaggtc aaatccgacg ttcttctttt cggctttttc ccattgcaaa ccttcgtttc
     2461 ccattctacc ctgatttaca ctctgcccca ggccaagacc cgggaattcg ccgccgccca
     2521 tggttatgaa actttgataa aggaatctgc tgttgcctaa tttatcgttc ccggtaatcc
     2581 cgtaggaagc cctaaacttt aaatgagtaa tcaccgggtt tccttttagg aatccttcgt
     2641 tacttgccac ccatcccaat gaaaaagcgg ggaacgaacc gaagcggttt ttaggagcaa
     2701 attgttccga accgttgtat ccaaagttaa attcacctaa atagcggtta tcgtagtcgt
     2761 aggttaaacg tcccacatat ccaatcacat tataaggcag gtcggcactc caaccttccc
     2821 agttatcgcg ttgatgcagg aacatcccgc tcacattgtg aaggccgaaa cttcgtgcgt
     2881 aattcaacga tacctgataa ttcatgaaat aataggtgtt catgttttta gacagtcgga
     2941 tagatttatc ctgattatca cggatcggag aatatcctga cttttgggtg gcgttccggg
     3001 ctacataaaa agcataagta tttagtgacc tgactccctg gcgggtggta gctgcatggc
     3061 tgtcgaatgc aatcatccct ttggcgctca gtccttttgt tatgaaacct aatccccagt
     3121 cgagagctaa cgatgaattc agaaccgttg aatattcctg acgatagccg cgccgattaa
     3181 tttccccgta agtattccgg tcttgtcccg attgggcaat aatttcattt tcggggacac
     3241 catatccggc cacggttaca ggacccggat ctgtaggtgg tgttccccag gtgtaagcca
     3301 tcatattttg aaccataccg ttcacatcgc caccaaataa gtcgacggtc tgcggagaat
     3361 tcatttgttg cagataggtg gcaatattca aagaagcctt caggttttgt gcaattttat
     3421 agtcgatatt gcctctgaaa ttgtatctgt ccatgctata acttggatca taacccagga
     3481 agcttttcgg ttcggtttta aaatttccgc cctgtccaat ataacccgca ttgaggaagt
     3541 agttaaagtc ttttccgcca ccgttgaagt tggcatttac acgggtttgg ggtgcccagt
     3601 ctctgaaata atcgtgataa acatcgcggt caggataaaa taccggatct tcaccacttt
     3661 tatacttgtc aatcatatac tgggtaaaag gcatttcatt ttcggtcgca ttgggatttc
     3721 cgttgagaaa agcctgattt cttaattctg caaattccca ggaatggatc cggtcattat
     3781 ggaccaggaa ttgctgaaaa cttttatcga cggtaacgct tagttttgat tttccttcct
     3841 cgccgcggcg tgtggtaata agaattacgc cgtttgcccc gcgcacccca aatacggcgg
     3901 tggccgaagc atctttgagg atagagattg aagcaatttc gttaggatcg agtttgtcga
     3961 tgttgcttct gggaatccca tcaatcagga tcaacgggga agcgtcgtta agtgtgctga
     4021 ctccgcgaag gtagaggttt accacatcgt tacctggttg tccggacgat tgcataacgg
     4081 tgagaccggg taattgtcct gccagcgcca cagaaaggtt tggagcagaa cttcgtttca
     4141 gatcttttgt catcacgctc gaaattgcag cagtaaccga ctctttcttt tgaatgccat
     4201 aacctacagc tactacttct tccaagccaa ccgtttcttc ttccataatc accgtaaagt
     4261 cggccttatt cccaatctgg atttcctggg ttttcattcc tacgaaagag aataccagta
     4321 ctttagcttc gccggtgtag atcaggcgga aattcccgct ggcatctgtg attacaccga
     4381 aagttgttcc ttttattatc actgaagtcc ctggaactgg catgttttgt ttgtccagaa
     4441 cttttcctgt gatctctttt ttttgctctg caggtatggt tgcctgaatg acttcagtct
     4501 ctggattttt cttgccaatc acaatttgcc tatcaaaaac agcaaaggta tttccggtgg
     4561 tctccagaac tttttcgaga attttttcaa ctgtgagatt ttctgctttg atgctgattc
     4621 ttcgatttaa atccagggca tcgtcgtaga aaacaaaaat gaattcactt tgcttttcaa
     4681 tttccttaat taactgtttg atagtggtat tctgcaagtt aagagtcagc ttggtattct
     4741 gagaataact aacactggca cttaccgtcg cagttgtgat gagcagaaaa aacagacata
     4801 ttttcatcgt cagccataat ctttcaaagt gccgaaatct cggcacccga aacttgtttt
     4861 ttttcataaa tttggttcgt ttttaatttg caattaatta atttacagca cttgcgatta
     4921 aaacgccgga cgggtcatgt gccctatgac ctgtcctttt ttgttttaat cagatcattc
     4981 gttaggtttt gttcggtttt ttataggcat tttcttttga cgttaatact attagttttc
     5041 ttatctctgt tgggttatca cgattgtatc ctcttgcatt tcacaagtaa ttggcgcggt
     5101 cgaactaata gcatggaata tctccacaca ttcgtttttc agatcaagtt ttcctgttaa
     5161 actcattttt ttggattcct catctttgaa ttgaattttt acgttgtaat acctggatag
     5221 ctttgtcgca atagattcca gcgattcctt ttcgcaatac atccatccat ctttccagga
     5281 tatgtaggga agtacatctg tatcatcaag cgaagcagcg tgtgagtttt tctcatacac
     5341 aagtcgctga ttcggcttta tgaccattgt ttttgagtcg accgttgcct gtattccacc
     5401 ttcaaccaat actaccgaag ccgaaagatc ttcaggatag gctgaaacat tgaattttgt
     5461 tccgagaacc tcgatctgca atttgtcggt taccacttta aagggtttgg agggatcgtg
     5521 tgcaacttcc agatagg
//
LOCUS       DAOSDR010000218         5531 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrimonas sp. isolate AMR_MDS_1826
            NODE_5269_length_5531_cov_3.338568, whole genome shotgun sequence.
ACCESSION   DAOSDR010000218 DAOSDR010000000
VERSION     DAOSDR010000218.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863355
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrimonas sp. (wastewater metagenome)
  ORGANISM  Petrimonas sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Dysgonomonadaceae; Petrimonas.
REFERENCE   1  (bases 1 to 5531)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5531)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:10:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,720
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 35
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5531
                     /organism="Petrimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5269_length_5531_cov_3.338568"
                     /isolate="AMR_MDS_1826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2023866"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..976
                     /gene="rhaT"
                     /locus_tag="PLI30_09020"
     CDS             <1..976
                     /gene="rhaT"
                     /locus_tag="PLI30_09020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019540940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="L-rhamnose/proton symporter RhaT"
                     /protein_id="HOI79731.1"
                     /translation="SSYVPINKVKNWSWENFWLVQGIFAWLVFPVLGAFLANSFPELV
                     QVYSANSGAALQSIGYGVLWGIGGLTFGLSMRYLGIALGQSVALGTCAGFGTLIPAVL
                     AGDNLFSPKGLVLLLAVVVTLVGIALVGYAGSLRSKNMSEEERRKAIKDFALKKGLLI
                     ALFAGVMSACFSLGLSAGIPIKEAAIAAGAKEIFAQNPVTLLVTIGGFFTNLVYCLYM
                     NSKNKTSGEIRKTSKSVLVNNLLFCALAGLLWYSQFFGLGMGQSFFEPGSVMMAFSWS
                     ILMSLNVVFSNVWGIILKEWKGAGKKAVTFLVIGMAVLIFSLIIPNLF"
     gene            1093..1992
                     /locus_tag="PLI30_09025"
     CDS             1093..1992
                     /locus_tag="PLI30_09025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019540950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HOI79732.1"
                     /translation="MRYNDLGIDFKYLLVSEKDKEFGLTVNTVGFSHIAPNTLYPSTD
                     HPKSYYFNPQKGRILSEYQFVYICKGRGVFSSATTKKTSIEKGQVMVLFPGQWHSYQP
                     VKETGWNEYYIGFEGSIIDSVIQKSFISEQNQVLDIGTNEELINLFLTAIRVAKEDKK
                     SAQQYLAGIAFHILGMILSLSQNKSYDANESTQVIERAKIIMRENIHKDIDVKEIASN
                     LGTSYSWFRKFFKEYTGYPPAQYFQELKLRKAKELLTETSLPIKEIAYELNFSSIEYF
                     LSFFKQRVRITPSEYRKLNGNDK"
     gene            complement(1996..3267)
                     /gene="serS"
                     /locus_tag="PLI30_09030"
     CDS             complement(1996..3267)
                     /gene="serS"
                     /locus_tag="PLI30_09030"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HOI79733.1"
                     /translation="MLTLKVINEDREEVIRKLAKKRFAAEDIINQIVALDETRRSSQT
                     LLDSQLSEINLLSKSIGGLMKEGKKAEAEIAKQQVSELKELSKLNEAGLREAEAAIQN
                     LLVIIPNLPHDSVPEGKSAEDNLVERSGGNIPQLSTDALPHWELAKKYDLIDFEWGVK
                     ITGAGFPVYKAKGSRLQRALINFFLDNARDAGFVEIQPPYVVNAASGFATGQLPDKEG
                     QMYHVGLDDLYLIPTAEVPVTNLYRDVILDEKDLPVKNTAYSACFRREAGSYGKDVRG
                     LNRLHQFDKVEIVCIDKPENSYQRLDEMVRYVQTLVEKLELPWRILRLCGGDMGFTSA
                     LTFDFEVYSAAQQRWLEVSSVSNFESYQANRLKCRYRNEDRKTQLCHTLNGSALALPR
                     IVAALLENNQTPEGIIIPAALVPYTGFEVID"
     gene            complement(3373..3510)
                     /locus_tag="PLI30_09035"
     CDS             complement(3373..3510)
                     /locus_tag="PLI30_09035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI79734.1"
                     /translation="MKNSVFKDILVVAAFSAVSYIVMRNYQRKKRRDRQMRDFVHFQL
                     F"
     gene            complement(3651..4970)
                     /gene="tilS"
                     /locus_tag="PLI30_09040"
     CDS             complement(3651..4970)
                     /gene="tilS"
                     /locus_tag="PLI30_09040"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005856304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HOI79735.1"
                     /translation="MLQKIKSYIAQKRLLENDNVLITGLSGGADSMALLDIMTLLGYR
                     CIAAHCNFHLRGEESEEDARFVKKWCKESDIEFTTIDFDTRQYAADKKISIEMAAREL
                     RYAWFEIVRKQFNADAIAVAHHKDDSVETVLMNLIRGTGISGLSGISPQNGKVIRPML
                     CVSRVEIENYLVERQIPFRTDSTNLEDIYTRNHIRLNVLPMLQKINPSVNETIFRTAE
                     NLAEAEKIYKKAVHADIEVVLKNNRIDIEALKLSASPLSVLFEILSPLGFHPAVIDDV
                     KAGLDASPGKQFFSPTHRLIKDRTCFLIDKIKKTESDDPIYFIDAVSQEISTPVNLAI
                     KLTGVPKLIDKKSRMLYADVAKLQFPLKLRKWQAGDRFIPFGMKGKKKLSDFFTDQKF
                     NLKEKEETWLLLSGEEVVWVVGHRADNRFKITPATERVLQIELKTMK"
     gene            5122..>5531
                     /locus_tag="PLI30_09045"
     CDS             5122..>5531
                     /locus_tag="PLI30_09045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005857329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HOI79736.1"
                     /translation="MLDKIHSLLSEIDQFSASDADELEALRIKYLSKKGIISVLMDDF
                     RNVAPEQKREVGMKLNELKQKAQEKILSLKEMFNGNKEKNVDIDLTRTAYPVALGARH
                     PISIVKEEICDIFKRLGFSIAEGPEVEDDWHVFSS"
BASE COUNT         1622 a         1187 c         1099 g         1623 t
ORIGIN      
        1 aagctcctac gttcctatca acaaagttaa aaattggtcg tgggaaaatt tctggctggt
       61 gcagggaatt ttcgcttggc ttgtttttcc tgttttagga gcattcctgg caaattcctt
      121 tccggaactg gtccaggtct attcagccaa ttccggtgca gctttacaat cgattggata
      181 cggcgtcttg tggggaattg gcggattaac ttttgggctc agcatgcggt acctggggat
      241 cgcacttggc caatctgtag ctttggggac atgtgctggc tttggaacgc taatccctgc
      301 tgttcttgcc ggggataatc ttttctctcc aaaaggattg gtacttttac tggctgttgt
      361 tgtaaccttg gtaggaattg cattggttgg atacgccggc agtttacgct ctaaaaacat
      421 gtcggaagaa gaacgcagga aagccataaa agactttgcc ctaaaaaaag ggttgctgat
      481 agcgttgttt gccggagtaa tgagtgcttg tttcagttta ggcctgagtg ccggaattcc
      541 gataaaggaa gccgctattg cagcgggggc aaaagaaatt ttcgctcaaa accccgtaac
      601 acttttggtg acgattggcg gttttttcac taatctggtc tattgcctgt acatgaattc
      661 gaaaaacaag accagcgggg aaattcggaa aacatccaaa tcggtacttg ttaacaacct
      721 tctcttctgt gccttggccg gtttactctg gtattctcag ttcttcggat tagggatggg
      781 gcaaagtttt tttgaaccgg gcagtgtgat gatggcattc tcctggagca tcctgatgtc
      841 cttaaacgtt gttttcagta atgtgtgggg tattattctg aaagaatgga aaggagccgg
      901 taaaaaagcc gtcacatttc ttgtcatcgg catggccgtc ttaatatttt ctttaataat
      961 tccaaatcta ttctaattca ttctctcatt ttttcagaag cacaatgcaa tcaattgagt
     1021 ttgcattgtg cttcattttt ttttgtaaat ttgtaaaaaa aagaaaagct acataccaca
     1081 aaaacagaaa aaatgagata caacgacttg ggtatagatt ttaaatactt gttggtcagc
     1141 gagaaagata aagaatttgg attaacagta aatactgttg gtttttcgca cattgcaccc
     1201 aacacattat atccttccac tgaccacccc aaaagttatt attttaaccc ccagaaaggg
     1261 agaatactat ctgagtatca atttgtttac atctgtaaag ggagaggggt tttttcatct
     1321 gccacgacaa aaaaaacatc cattgaaaag ggtcaggtaa tggtcctttt tccaggacag
     1381 tggcactcgt atcaacccgt aaaagaaacg ggatggaacg agtattatat tggttttgaa
     1441 ggaagtataa tcgactctgt aatccaaaag agctttattt cagaacaaaa tcaggtttta
     1501 gatattggta cgaacgaaga gcttatcaat ctgtttttaa ccgctatcag agttgccaag
     1561 gaagacaaaa aatcggccca acagtatctc gccggaatag cttttcacat cttaggaatg
     1621 attttatcac tttctcaaaa caaaagttat gatgctaatg aatcgacaca agtaattgaa
     1681 cgtgccaaga tcatcatgag agaaaacatc cataaagaca tcgatgtaaa ggaaattgca
     1741 tcaaatctgg gaacaagcta ttcctggttc agaaaatttt tcaaagaata tacgggctat
     1801 ccgcccgcac aatattttca ggaattgaaa ctccgaaaag caaaagaact gttgacggaa
     1861 acaagtttac ctatcaagga aattgcctat gagctcaatt tcagctccat cgaatatttt
     1921 ttatcctttt tcaaacaacg ggtcaggata actccttctg aataccgcaa attaaacggc
     1981 aacgataaat aataattaat caattacctc aaatcctgta taaggcacca atgcagccgg
     2041 tataattatt ccttctgggg tttggttgtt ttccagaagt gcggcaacga tacgtggcaa
     2101 agccagtgcg ctcccgttga gggtatggca tagttgtgtt tttcgatcct cgttgcggta
     2161 tcggcatttc agccgatttg cctggtaact ctcgaaatta gatacggaac tcacttccaa
     2221 ccacctctgt tgtgccgcgg aatagacttc aaagtcgaac gtaagcgcag atgtgaatcc
     2281 catatcaccg ccacacaaac gtagaatgcg ccagggaagt tccagctttt ccaccaaagt
     2341 ttgcacataa cggaccatct catccaaccg ctgatacgaa ttttcaggct tgtcaataca
     2401 cacaatttct actttatcaa actgatgcaa acggttcaat ccacgcacat cttttccgta
     2461 ggatcctgcc tctcggcgaa aacaagcaga ataagccgta ttctttaccg gcagatcctt
     2521 ttcatccaaa atcacgtctc ggtaaagatt ggtaaccggc acctcagccg ttggtatcag
     2581 gtacaaatcg tctaatccta catgatacat ttgtccttcc ttatcgggta actgcccagt
     2641 cgcaaagcca gatgccgcat ttacgacata aggcggctga atttcaacaa atccggcatc
     2701 acgggcattg tcaagaaaga aattaatgag tgcgcgctgc aggcgactac ctttggcttt
     2761 gtaaacagga aacccggcac cggtaatctt caccccccat tcaaagtcta tcaggtcata
     2821 tttttttgcc agctcccaat gaggaagagc gtcagtagag agttgcggaa tgtttcctcc
     2881 cgaacgctct acaagattat cttcggcaga tttaccttca ggcaccgaat cgtgcggtaa
     2941 attaggaata ataaccaaca aattttgaat agctgcttca gcttctctca gcccggcttc
     3001 attaagtttg gaaagctcct tcagttcgga aacttgttgc ttggcaattt ccgcttcagc
     3061 cttcttgcct tctttcatca gccctccgat cgacttcgac aggaggttga tctccgacaa
     3121 ctgactatcc agaagagttt gagaactacg acgtgtttcg tctaacgcca caatttgatt
     3181 aattatatct tcagcggcga atcttttttt ggcaagtttg cgaatgactt cttcgcgatc
     3241 ttcgtttatt actttgagtg taagcataaa aaaaacagtt tcaactattt tgactgagaa
     3301 tatgcaaagg tagcaaaaat tctcgcgccg aaacagttgt aacacaaaat atatttaccg
     3361 gtaaagcggc aatcagaaca attggaaatg aacaaaatcc ctcatttgac ggtctctcct
     3421 tttctttctc tggtagtttc gcatcacaat atatgaaact gccgaaaagg ctgccaccac
     3481 caagatatcc ttaaatacac tatttttcat tatcgtagtt ttttagttat aaccgtttta
     3541 tctgcttttg tgtttaaaca aaatcaattt ttccgctatg aagttacata aaaatacctt
     3601 ttcgtcaaaa aataagggtg aaaattatcc tgaaaatagt tttattcaag ctatttcatc
     3661 gtttttaact caatttgcaa aacacgttca gtcgctggtg taattttgaa ccggttatcc
     3721 gcccgatgcc ccacaaccca aaccacctcc tcacccgaaa gcaaaagcca tgtttcttcc
     3781 ttttctttca ggttaaattt ctgatcagtg aaaaaatcac tcaacttctt tttgcctttc
     3841 attccaaagg gaataaacct gtcgccggct tgccatttcc gcaacttgag ggggaactgc
     3901 agcttagcga catcggcata taacatacga ctctttttat cgattagttt cggtacacct
     3961 gttaatttaa tagctaaatt taccggagtg gatatttctt gcgataccgc atcgataaaa
     4021 tagattgggt catcggactc cgtttttttt attttatcga ttaggaaaca cgtacgatct
     4081 tttatcaacc gatgtgtagg tgaaaaaaac tgcttccccg gcgaagcatc caatccagcc
     4141 ttcacatcat cgatcacggc aggatgaaac cctaaaggtg acaggatctc aaacaaaacc
     4201 gataaggggg aagcggatag ttttagcgcc tcaatatcta ttctgttatt tttcaaaaca
     4261 acttcaatat ccgcatgaac tgctttttta tatattttct ctgcttcggc caagttttca
     4321 gcagtacgga aaatggtttc gttgactgac ggattgattt tctgcaacat cggcaaaaca
     4381 tttaaccgta tgtgattccg ggtataaata tcttcgagat tggtactgtc agtcctgaaa
     4441 gggatttgac gttccaccag gtaattttcg atttcgaccc gggagacaca aagcatcgga
     4501 cggataactt ttccgttttg aggcgaaatt ccgctcaatc cacttattcc cgtaccgcgg
     4561 attaaattca tcagtaccgt ttccaccgaa tcatccttgt gatgagctac ggcaattgca
     4621 tcggcattaa attgtttcct cactatttca aaccaggcgt aacgcaattc acgtgctgcc
     4681 atctcgatgg aaattttttt atcggcggca tattgccggg tatcgaagtc gatggttgta
     4741 aattcaatat cactctcctt acaccatttc tttacaaagc gggcatcctc ctccgattct
     4801 tctccccgaa gatggaaatt acaatgtgcg gcaatacacc ggtaacccaa taaagtcatt
     4861 atatccagta aagccatgga gtcagcgccc ccactaagcc ccgtaatcaa cacattatcg
     4921 ttctccaaca aacgtttttg ggcaatatag gattttattt tctgaagcat cggctcaaga
     4981 tatacgaaca ggtatttttt gtaaatgcaa aaatacgata tgtttttcaa aaattgcggg
     5041 aaatgatttc tttcttcaaa atcaaggtaa atagccaaga aaaatgtatt tttgcataaa
     5101 attaaagaca ataaagatcg tatgttagat aaaattcact cccttctttc tgaaatagat
     5161 caattttcag catcggatgc cgatgaactg gaagcattac gcatcaaata cttaagcaaa
     5221 aagggcatca tttctgtgct gatggacgat ttcagaaacg ttgcccccga acaaaaacgc
     5281 gaagtgggta tgaagctgaa cgagctgaaa cagaaagccc aggaaaaaat tctttcgttg
     5341 aaagagatgt ttaacggcaa caaagaaaaa aacgtggata tcgacctgac ccggacggct
     5401 tatccggttg ctctcggagc acgtcatccg atatcgattg tgaaagaaga aatttgtgat
     5461 atttttaaac gtctgggatt ttccatcgcc gaagggccgg aagtggaaga cgactggcat
     5521 gttttttctt c
//
LOCUS       DAOSDR010000219         5517 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrimonas sp. isolate AMR_MDS_1826
            NODE_5282_length_5517_cov_3.740022, whole genome shotgun sequence.
ACCESSION   DAOSDR010000219 DAOSDR010000000
VERSION     DAOSDR010000219.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863355
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrimonas sp. (wastewater metagenome)
  ORGANISM  Petrimonas sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Dysgonomonadaceae; Petrimonas.
REFERENCE   1  (bases 1 to 5517)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5517)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:10:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,720
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 35
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5517
                     /organism="Petrimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5282_length_5517_cov_3.740022"
                     /isolate="AMR_MDS_1826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2023866"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..917)
                     /locus_tag="PLI30_09050"
     CDS             complement(<1..917)
                     /locus_tag="PLI30_09050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006843198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HOI79737.1"
                     /translation="MKKAVILAGLLALNLSLFAQADEQTDSLKNIHLEEVVVSSTRAG
                     KNTPMAYSNVSQTEIRKENAARNIPAILQGIPSLVFFSEDGSGVGNTSMRIRGTDATR
                     INVTLNGMPLNNPESQEVYWVNLPDLSSSLQSIQVQRGVGTSTNGAAAFGASISMKTA
                     GARSTAYGEASTSAGSYHTFSSAIAAGSGILKNGLSLDARYSRNTGDGYIRNGFVDHS
                     NLYAALSHYTDNQLLRLSYINGRQKTGITWEGISPEDLEKEGRRYNPAGKYIDEADNV
                     HYYDNETDNYFSHIVQLLFTRDLNSHLSL"
     regulatory      complement(1092..1188)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            1348..3615
                     /locus_tag="PLI30_09055"
     CDS             1348..3615
                     /locus_tag="PLI30_09055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019537872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0015968 - stringent response [Evidence
                     IEA];
                     GO_process: GO:0015969 - guanosine tetraphosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RelA/SpoT family protein"
                     /protein_id="HOI79738.1"
                     /translation="MSEEDKLAAEDRMIEDEFQALINDYLNSNHRRKVEVITKAFNMA
                     KEAHKGARRRSGEPYIMHPLAVARIISREMGLGSTSISAALLHDVVEDTDYTVEDLEV
                     MFGDKIAQIVDGLTKISHGMFGENVSAQAENFRKLLLTMSEDIRVILIKIADRLHNMR
                     TLSSMTPAKQFKITGETMYIYAPLAHRLGLFAIKTELEELCFKYEHPESYAELSNKVE
                     TSAQERFEIYESFAEPVVKALDKMNIEYEMRARVKSLYSIWKKMQAKGIDFSEVYDLF
                     AVRIIFEIYPGMDEKKQCWDIYSAITDIYKLRPDRIRDWVSHPKANGYQALHLTVMGP
                     DGKWIEIQIRSRRMDDIAEKGFAAHWKYKENRIEEDNELDKWLKTIQEVLANPNPNAI
                     DFLDTVKMNLFASEIFVFTPKGEIKTLPLGATALDFAYNIHTRIGDHCLGAKVNHTLV
                     PLSQKLKSGDQVEILTSQSQQPQPEWLNYVTTAKARTKIEAAIRRQRRRISERGQKML
                     EEFVGEEQLDNTLYNKTLNYYKVEKREDLHYGLGNGDLKLPDSRDKLLKQRETVEPKQ
                     DNLFVRYMKQAMSGFKKAPEESRSDIAVVSPAKTGEIDKKAIYKLHEVSFKKNFIVAS
                     CCNPLPGDDVFGFITDKNEVEVHKRSCETGIKLKSRFGERILATEWGDYKQYSFLSAI
                     VFTGIDRIGILNTILSKLAEDIVVNIKSVNVTSKDGIFEGVFHVHVHSVEDVNVLISK
                     IAKVDGVTTVHRALI"
     gene            complement(3626..4006)
                     /locus_tag="PLI30_09060"
     CDS             complement(3626..4006)
                     /locus_tag="PLI30_09060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI79739.1"
                     /translation="MKSYKLTPLDELHLEKKKLREERAISGQRLSYQIQYLADNWGSM
                     LTKGVTTSIKNKFSETVDNLSYGSSSSVVPFKTKKPGNPWINLALSNLPLISGIAWSV
                     AKPAILAFAVKKITGRLFGGKKRK"
     gene            complement(4006..4386)
                     /locus_tag="PLI30_09065"
     CDS             complement(4006..4386)
                     /locus_tag="PLI30_09065"
                     /inference="COORDINATES: protein motif:HMM:NF018980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HOI79740.1"
                     /translation="MEDKTVTSLFEEMKSDVTGYVTNTIEIVKLEAFEKVSKGAASTA
                     ITLFLVSFIFLILALALLTLGFYLADVFDSNWKGFGIVTLGTIALTLVLLLIKKPLKH
                     SIINSVIKFLQRQEDEEVKFTTKS"
     gene            complement(4399..4641)
                     /locus_tag="PLI30_09070"
     CDS             complement(4399..4641)
                     /locus_tag="PLI30_09070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019537869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HOI79741.1"
                     /translation="MKSEAKLLLGLGIGIALGAAVGYIMGSDKKEEWLEQANAFADKV
                     RANVKSAIYKGKSEVSDLKEDIDDIAEIAKKELAKH"
     gene            complement(4762..>5517)
                     /locus_tag="PLI30_09075"
     CDS             complement(4762..>5517)
                     /locus_tag="PLI30_09075"
                     /EC_number="2.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005867084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MiaB/RimO family radical SAM
                     methylthiotransferase"
                     /protein_id="HOI79742.1"
                     /translation="GYREATLLGQNVNSYKFEDDGRVIDFSDLLAMVAEEAPQMRIRF
                     TTSHPWDMNDKTLETIAKYKNLCNYIHLPVQSGSSRMLKLMNRRYDRKWYLERIAAIK
                     RILPGCGLSTDIMCGFHSETEADHQETLSLMREVGFDSAFMFKYSERPGTYAAKKMED
                     NVSEEVKGRRLQEIIDLQLELSQKSNEQDVGKEFEVLVEGFSKRSREQLFGRTEQNKV
                     VVFDKQKHRIGEFVKVKITGFTAATLFGEPSGN"
BASE COUNT         1491 a         1210 c         1239 g         1577 t
ORIGIN      
        1 ttgagggata gatgggagtt gaggtcgcgc gtaaacagaa gttgtacgat gtgtgaaaag
       61 tagttgtcgg tttcgttatc gtaataatgc acgttatcgg cttcgtcgat gtattttccc
      121 gcggggttgt atcttcttcc ctctttttcc aggtcttcgg gggagattcc ttcccaggtg
      181 atgcctgtct tctgcctacc attgatataa ctcaaccgga gcaattgatt gtccgtataa
      241 tgggaaaggg cagcatacag gttgctgtga tctacaaatc cgttgcggat atatccatca
      301 cccgtgtttc gtgaataacg tgcatccagc gacaggccgt tctttagaat cccgcttcca
      361 gcagcgatag cggacgaaaa tgtgtgataa ctgcctgctg atgtggatgc ttcgccatat
      421 gccgttgacc gtgcgcctgc cgttttcatg gagatgctgg cgccgaaagc ggcagcaccg
      481 tttgttgagg ttcctacgcc gcgctgtact tgaatacttt gcagggacga tgataaatcg
      541 ggtagattta cccagtaaac ttcctgactc tcaggattgt ttaacggcat gccgtttagc
      601 gtaacattga tgcgggtggc atccgttccg cgtatgcgca tggaggtgtt tcccacgccc
      661 gatccgtctt cgctgaaaaa caccagcgat ggaatgccct gcaggattgc cgggatattt
      721 cgggctgcgt tttcttttct gatttcagtc tgggaaacat tgctgtacgc catcggcgtg
      781 ttctttcccg cccgggtgga agagaccacc acttcttcga gatgtatgtt ctttaaactg
      841 tcggtttgtt cgtctgcctg agcaaacaag gaaaggttga gggccagaag cccagcaaga
      901 ataactgcct ttttcattta caagtagttt ttatgacaca agaaacaagt gtttttttgc
      961 gtcggatgag taaaataaaa aaggggattt ttgaaagatt tacctaaaaa caaaatcacc
     1021 aagctacaat gttattcaat tgtacggcta ataacgcgga gcgtatacct gttgaatatc
     1081 cattgatttt tttgtgatct ttttttgttt ccctccgccg gcattatccg gatcaggtat
     1141 ttgggtatga tctcagcccg ttgttatgtg gcacccctgt atttgacaaa acaaagctac
     1201 ttcatttttg ttcaccaaac aataaaaacc gcaattttta acgttccagc aataaaaaat
     1261 ctgttacgtg tgactttgat agctgtttta tttgtaattt cgttccttca ttgcaacgtt
     1321 caattacggt atgttttaaa tgaaaatatg agcgaagaag ataaacttgc ggcagaagat
     1381 cggatgatcg aggatgagtt tcaggcactt attaacgatt atttaaattc gaaccaccgg
     1441 cgtaaagtgg aagtcattac caaagccttt aatatggcaa aggaagccca caaaggagca
     1501 cgccgtcgtt cgggcgagcc gtacattatg catccgctgg ccgttgcaag aattatttcc
     1561 cgcgaaatgg gactgggctc aacctccata tcggctgcat tgctgcacga tgtggtggaa
     1621 gacaccgact atacggttga ggaccttgaa gtaatgttcg gcgataagat tgcgcagatc
     1681 gttgacggat taacaaaaat ctcacacggc atgtttggtg agaacgtatc ggctcaggcg
     1741 gaaaattttc gtaaactcct gttgacgatg tcggaagaca ttcgtgtgat cctgataaaa
     1801 attgccgatc ggttgcacaa tatgcggacg ttgtcgtcca tgactcccgc aaagcaattc
     1861 aagataaccg gcgaaaccat gtatatttat gcccctttgg cgcaccggct gggacttttt
     1921 gccatcaaaa ccgagctcga agaactttgc ttcaagtacg aacacccgga atcttacgct
     1981 gaactttcga ataaagtgga aacttctgcc caggagcggt ttgaaattta tgagagtttt
     2041 gccgagccgg tagttaaagc actcgataag atgaatatcg aatatgagat gcgtgcccgg
     2101 gtaaaatcgc tttattccat ctggaagaag atgcaggcca aaggtatcga tttcagcgaa
     2161 gtgtacgatc tttttgccgt ccgtatcatc tttgagattt atcccgggat ggatgagaaa
     2221 aaacaatgct gggacatcta ttctgctatt acggatatat acaaactccg ccccgaccgt
     2281 attcgagact gggtgagcca tcccaaggcc aatggttacc aagcgcttca cctgacggtg
     2341 atgggccccg acgggaaatg gatcgaaata cagattcgca gccggcgaat ggacgatatt
     2401 gcggaaaaag gttttgctgc ccattggaaa tacaaggaga accggataga ggaagacaac
     2461 gaactggata aatggctgaa aacgattcag gaggtattgg ccaatcccaa ccctaacgcc
     2521 atcgattttc tggatacggt aaaaatgaac ctgttcgctt ccgagatttt tgttttcacc
     2581 ccaaaaggcg aaatcaaaac ccttcccctg ggtgctaccg ccctcgattt tgcatacaac
     2641 attcacaccc gcataggcga ccactgcctg ggagcaaaag tgaaccatac gctggttccg
     2701 cttagccaga aactgaaaag cggcgatcag gtagagattc tgacgtccca atcgcaacaa
     2761 ccacaacccg agtggctcaa ttatgttaca accgccaagg cccgaaccaa gatagaagcc
     2821 gcaatccgcc ggcaaaggag aagaataagc gagcgaggtc aaaaaatgtt ggaagagttt
     2881 gtcggagaag aacaactcga taataccctg tacaacaaaa ccctgaatta ttataaagta
     2941 gaaaagcggg aagatttgca ttatggcctg gggaatggtg acctgaaact tcccgattcc
     3001 agggataagt tgcttaaaca acgggaaaca gtggaaccca agcaagacaa cctgtttgtt
     3061 cgttatatga aacaggccat gagcggtttt aaaaaagccc ctgaggaaag caggagtgac
     3121 attgctgttg taagtcctgc aaaaaccgga gaaatagaca agaaggcgat atataaactg
     3181 cacgaggtga gtttcaagaa aaattttatt gttgcttctt gctgcaatcc gttgcccggt
     3241 gatgatgttt ttggatttat aaccgacaaa aatgaagtgg aagttcacaa acgctcctgc
     3301 gaaacaggca taaagttgaa atcgcgtttt ggggaaagaa tcctggcgac ggagtggggc
     3361 gactataaac agtattcttt tctttcagcc attgttttta cgggcattga ccggataggc
     3421 atcttgaata cgatattgtc taaactcgca gaggatattg tggtaaacat aaaaagtgtg
     3481 aacgttacgt cgaaagacgg tatttttgaa ggtgtttttc atgttcatgt ccacagtgtg
     3541 gaagatgtta atgtcctgat ttcgaagata gcaaaagtgg acggtgtaac caccgtccac
     3601 agagccttaa tttaatcaag tagttctatt ttcgtttctt ccctccaaac aacctgccgg
     3661 taattttttt tacggcaaag gccagaatgg ccggcttggc aacactccag gcaattccac
     3721 ttattaaagg taagtttgaa agagccaggt ttatccaggg gttacccggt ttttttgttt
     3781 taaagggtac cacggacgac gatgagccgt aagacaggtt gtccaccgtt tcactgaact
     3841 tgttcttgat actggtcgtt acgcctttgg taagcataga tccccagtta tccgccagat
     3901 actggatctg ataagatagg cgttgaccag aaattgcacg ttcctcacgc agtttcttct
     3961 tctccagatg gagctcgtcg agtggagtaa gtttgtaact tttcatcagc tttttgttgt
     4021 aaatttaacc tcttcgtctt cctgacgttg cagaaatttg attacagaat tgatgataga
     4081 gtgtttcagc ggctttttta tcaacaacaa aaccagagta agagcgatag tgcctaaagt
     4141 gacaatgcca aaacctttcc aattactgtc aaaaacgtct gccaggtaaa atccgagagt
     4201 gagaagtgcc aatgccaata tcagaaaaat gaaactgacc aaaaacagag taatggcagt
     4261 tgaagccgct cccttactca ctttttcaaa agcttcgagt tttacaatct caatcgtgtt
     4321 tgtcacataa cccgtgacat cgcttttcat ctcttcaaat aagctggtaa ccgttttgtc
     4381 ctccatctga gtttcctttt agtgtttagc caattctttt tttgctattt ctgcaatgtc
     4441 atcaatgtct tctttcagat cactgacttc gctttttcct ttatagatag cggattttac
     4501 attcgcacgt actttatcgg caaacgcgtt agcttgttca agccactctt cctttttgtc
     4561 gcttcccata atataaccga cagctgctcc aagcgcaatt ccaattccta atccgagtaa
     4621 taattttgct tctgatttca taattttgct tttttagatg ttaatagttt gttttctttg
     4681 aaatgttgtt ttcaacgttg cctatttagt aaaacgtttt ttttttgaaa atgtttactc
     4741 cctcgggaaa aagttgattt atcagttccc gctaggctct ccaaaaagcg ttgctgcagt
     4801 aaatccggta atcttgactt ttacgaattc tccaatcctg tgcttttgct tgtcgaaaac
     4861 aaccaccttg ttctgttcag ttcttccaaa gagctgttca cgagagcgtt ttgagaaacc
     4921 ctctaccaac acctcaaact cttttcctac gtcctgctcg ttgcttttct gcgaaagttc
     4981 aagctgcagg tcgataatct cctgcagacg gcggcctttt acctcctcgg aaacattgtc
     5041 ttccattttt ttcgcagcgt atgtgcccgg acgttcggag tatttgaaca taaaagccga
     5101 atcaaagcct acctcacgca taagtgatag tgtttcctgg tgatctgctt cggtttcact
     5161 gtgaaatcca cacataatgt cggttgagag tccgcaaccg ggaaggatcc gttttatagc
     5221 ggcaatacgc tctaaatacc atttcctgtc gtatctccgg ttcatcagtt tcaacatgcg
     5281 tgaactcccc gactgtacag gcaaatggat ataattgcac aaattcttgt acttggcaat
     5341 ggtttccaat gttttgtcat tcatatccca cgggtgcgat gtggtaaaac ggatacgcat
     5401 ttgaggtgct tcctcggcaa ccattgccaa cagatcggaa aaatcgataa ctcttccgtc
     5461 gtcttcaaac ttgtatgaat tgacgttctg tcctaacaaa gtggcttcgc gatagcc
//
LOCUS       DAOSDR010000220         5506 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrimonas sp. isolate AMR_MDS_1826
            NODE_5291_length_5506_cov_4.561181, whole genome shotgun sequence.
ACCESSION   DAOSDR010000220 DAOSDR010000000
VERSION     DAOSDR010000220.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863355
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrimonas sp. (wastewater metagenome)
  ORGANISM  Petrimonas sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Dysgonomonadaceae; Petrimonas.
REFERENCE   1  (bases 1 to 5506)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5506)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:10:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,720
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 35
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5506
                     /organism="Petrimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5291_length_5506_cov_4.561181"
                     /isolate="AMR_MDS_1826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2023866"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..428)
                     /locus_tag="PLI30_09080"
     CDS             complement(<1..428)
                     /locus_tag="PLI30_09080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI79743.1"
                     /translation="MSQQDDLLKNLRQTLSEQGIESFHVLEDLVPIEEQMEYFRYFDR
                     LRRENTPFVRDEEIAILFSADETIERKKKSLSRLASIPDVGAYRAIETYHSSPLEPEL
                     FNWSSMSLVGSRIILSSDLSGQQQIYVSSGLGGHEKKLRF"
     gene            606..1040
                     /gene="dut"
                     /locus_tag="PLI30_09085"
     CDS             606..1040
                     /gene="dut"
                     /locus_tag="PLI30_09085"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004170 - dUTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046081 - dUTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HOI79744.1"
                     /translation="MQVKIVNKSKHSLPEYATALSAGMDLRANIDEPVTLRPLQRSLI
                     PTGIYIQLSEGYEAQIRPRSGLAVKHGISIVNSPGTIDADYRGEIRVILVNLSDEDFI
                     INDGERICQMVIARHTRVEWLQVDDLDETERGSGGFGHTGKH"
     gene            1130..2860
                     /locus_tag="PLI30_09090"
     CDS             1130..2860
                     /locus_tag="PLI30_09090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019538019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI79745.1"
                     /translation="MNYNKYISGIIVSLVLFPVSLFSQVEGLILDETSRRKFDYFFYE
                     AVNAKTLGKYAESFDLLQHCYSMDSTNASVLIELGAYYSSLEEKNKALDFFRRAVKQD
                     PANYYYNMILAGLSKELDLKKEVIEIYNYLLKTYPEKVELYFELANVYGDNGELDKAI
                     QSLDSLQKYTGVSDAITLNKFRLYNMMDKKDRAFEEIQSVIDKNPDNIRYKLLMGDLY
                     LQDNQADKALGYYQQAKQIAPDEPSLILSMVNYYEKTNNKPAAVEELQTAITSSKMEV
                     ETKLQLLARYITLLRQSQQDIKTANPLFQSMFEQHPNNTRINMMYGDVLLLQEDKKGA
                     MEQFEIYTKDNPADPAGYEQMLRIVLPDTQALEKVVEITLTGIEHIPTAPQFYFYLGL
                     AKFQQKKYGEALSIYEQGLTNAEFQSPVIESDFYGQIGDIHHFLKNNNIAFENYEKAL
                     KLNPQNLPVLNNYSYYLSLERKSLDKAEQMSGITIKAEPTNPTYLDTYGWILFEQGAY
                     TMAKIYIEKAIEYGKEDLTAEVLEHYGDVLAVTGEKEKAVEQWKKAKELGSGSKTLNK
                     KIKRKEYIKE"
     gene            2857..3702
                     /locus_tag="PLI30_09095"
     CDS             2857..3702
                     /locus_tag="PLI30_09095"
                     /inference="COORDINATES: protein motif:HMM:NF025490.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4292 domain-containing protein"
                     /protein_id="HOI79746.1"
                     /translation="MNFRKQIIIFLLGITVVILGLHSCKSKKQIVAAAPVEEKVNVDL
                     FTDILNNQFSFKTFSSKLNLNLSSGTKSLSSKASLRIVKDKAIQLSVQPLFGVEMLRL
                     YADRDSLVLLDRMNKRYVKESIEDVKKMYPVGFDYKTLQSLLTNQVFVSGQTDVIYSD
                     YDKFSTGQLSDMHYLLKSVDKKSGIEYSFTIDGNDRIALTHLIEPKDHYSMNWRYDDF
                     VMESHKAFPRKMDVALASTRRKVNVGLEFSGITLDEDFDLQVSVPGSYTRATLSDIVK
                     ILTNN"
     gene            3702..5063
                     /locus_tag="PLI30_09100"
     CDS             3702..5063
                     /locus_tag="PLI30_09100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006800915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HOI79747.1"
                     /translation="MRRVFTIGLFLTLASTLFSQTPQIDQLQKQQKMLLEEIKSTNKL
                     FLDVKKETTTLLQRINLINKQIASRKEMMTLQNQEIAALEREQLRLESEINRLGESLK
                     QKQESYAKAVRVMQASRSRQNELFFVLSGKSLGESLRRMQYLRDYSKWRKSQAEEIKQ
                     QNVQLSARKDELAKARTGKQAALDALKAEQLRLQEEEHVRQSEMTEARGKQRELQRML
                     QDKQQQARKLDAQIEKLIAEEVARQEREAEARRLAAEAERKRKAEEVAARVRREAEKG
                     RKTEELARKEPDKTEDVKPAVKQPEPKIESATTADETFNLSKNFVSNRGNLPMPVTGT
                     ASIVGSFGQRKHSEWNVTTNSNGIDIQAQQGANIRAVFEGEVSKVFSVPGYNTCVIVR
                     HGDYYTFYGNIYDLFVKSGDKLKSGQSLGRIYTDPDTGVATMHFQLWQKTTKLNPAPW
                     LKR"
     gene            5120..>5506
                     /locus_tag="PLI30_09105"
     CDS             5120..>5506
                     /locus_tag="PLI30_09105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018694869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOI79748.1"
                     /translation="MNIKIRLIVMNFLQYAIWGAWLISLGAYLGGGLKFTGVQIGSFF
                     ATMGIASLFMPGVMGVIADRWIPAQKLLGISHLIAGFFLFLAAPQTEYAPLYTYVLLS
                     VMFYMPTIALSNSVAYTSLTSCGLETV"
BASE COUNT         1658 a         1136 c         1303 g         1409 t
ORIGIN      
        1 aagaaacgaa gttttttctc gtggccgccc agtcccgaag agacatagat ttgttgttgt
       61 ccggacaggt cggaacttaa gattatccgg ctgcctacca acgacatcga tgaccagttg
      121 aacaattccg gctcgagcgg gctgctgtga taggtctcta ttgcccggta cgcgcctaca
      181 tcaggaatgg atgctagcct cgacaagctt tttttcttcc tttcaatggt ttcatcggca
      241 gaaaaaagaa tagcaatctc ttcatcgcgt acaaaaggag tattctcccg cctcaaccgg
      301 tcgaagtaac ggaaatactc catctgttct tcaataggaa ccaggtcttc cagcacgtga
      361 aacgactcaa taccttgttc gctgagcgtc tggcgtaaat tcttcaataa atcgtcttgt
      421 tggctcatag cgttacaaag atatgcaatt ttttactcca cgaaaataat tgctccaaaa
      481 attaaaccgt tttttgaagg cccaatttcc ttaaatttta tcaattcgac aaatatttgc
      541 gcataaatga ctaaattcgc aggtttaaac aaaatccgtt ttgagagtct attccttagg
      601 atattatgca ggttaaaatt gtcaataaat cgaagcactc attacccgaa tatgctacgg
      661 ctttgtctgc cggcatggat ttgcgggcaa acatagacga accggttacg ttgaggccgt
      721 tgcaacggtc gcttatcccc accgggatat acattcagct ttccgaagga tatgaagcac
      781 agatccgtcc gagaagcgga ctggcagtta aacacggaat ctccattgta aattctccgg
      841 ggacgattga tgccgattat cggggtgaaa ttcgggtgat cttggtgaat ttatcggacg
      901 aagactttat cattaacgac ggagagcgta tttgtcagat ggtaatcgcc cggcataccc
      961 gggtagaatg gttgcaggtg gatgatttag atgagactga aagaggctcc ggcggattcg
     1021 gacatacagg taagcattag atgatttgta ataaggatca aaacgcgtgt tatgcgtagg
     1081 cacaatgtct tgtgcccttc aatagaggga cattatcaga ttaaaaagaa tgaattacaa
     1141 caaatacata agcggtatca tcgtttctct tgtgcttttt ccggtaagcc ttttttcgca
     1201 ggtagaagga ttgatcctgg atgagacaag ccggcggaag tttgattact ttttttatga
     1261 ggcggtcaac gccaagaccc tgggtaaata tgccgaatct ttcgatttgc ttcaacactg
     1321 ttattccatg gattccacta acgccagcgt gctgatagaa ttgggtgctt actacagttc
     1381 cctggaggag aaaaacaagg cgttggattt ttttcgtaga gcagtgaaac aggatccggc
     1441 caactattac tacaacatga ttcttgccgg attgagcaaa gagctagacc tgaaaaaaga
     1501 ggtcatagaa atttacaact atttactgaa aacgtatccc gaaaaagtag aactctactt
     1561 tgagcttgcc aatgtatatg gcgataacgg agaactcgac aaggccattc agtcgctcga
     1621 ctcgctgcaa aaatataccg gagtttcaga tgccataacg ctcaataagt tccgattgta
     1681 caacatgatg gataaaaagg atcgggcgtt tgaagaaatc cagtcggtta ttgacaaaaa
     1741 ccccgataat atccgctaca aacttcttat gggcgatttg tatctacagg ataatcaggc
     1801 agataaagca ttgggctatt accagcaggc aaagcagatt gctccggatg agccttcgtt
     1861 aattctctcc atggtgaatt attacgaaaa aaccaacaat aaaccggctg ctgtagagga
     1921 attgcaaaca gccatcacga gcagtaagat ggaggtggag acaaaactac aactgttggc
     1981 ccgttacatt actttattac gccagagtca gcaggatatt aaaacggcca atccgctttt
     2041 tcaatcgatg tttgagcagc atcccaacaa tacccgcatc aacatgatgt acggtgatgt
     2101 gttgttgttg caggaagata aaaaaggagc gatggagcag tttgaaattt acaccaaaga
     2161 caatccagcc gatcctgccg gatacgagca aatgcttcgc atcgttttac cggacaccca
     2221 ggcgcttgaa aaagtggtcg aaattaccct gactggaatt gagcatattc cgactgcccc
     2281 gcagttctac ttttacctgg gactggctaa gtttcaacaa aaaaaatacg gggaagcatt
     2341 gagtatctac gaacagggcc tgactaatgc tgaatttcaa agtccggtga tcgagtcgga
     2401 cttttacgga cagatcggcg acatccacca tttcctgaaa aacaacaata tcgctttcga
     2461 aaattatgaa aaagccctaa agctgaatcc gcagaacctg ccggtactta ataattacag
     2521 ctattattta tcgctggagc ggaaaagtct ggataaagca gaacagatga gcggaataac
     2581 catcaaggct gagcctacaa accccaccta cttggatact tacggatgga ttctgtttga
     2641 acagggtgcc tatacgatgg cgaaaatata tatagagaag gcgattgaat atgggaagga
     2701 agatcttacg gcagaagttc ttgagcatta tggcgatgtg cttgccgtaa cgggtgaaaa
     2761 ggaaaaagcc gtggaacaat ggaaaaaagc caaagagttg ggaagcggtt ccaaaacatt
     2821 gaataaaaaa attaaaagaa aagaatacat aaaagaatga atttcagaaa acagatcatt
     2881 atctttctgc ttggcataac tgttgttatt ctgggattgc attcgtgcaa atccaaaaag
     2941 caaattgttg ccgctgctcc tgtcgaagag aaagtgaatg tcgatttgtt taccgatatt
     3001 ttaaacaatc agttcagttt caagacgttt tcatcgaaac tcaaccttaa tctttcatcg
     3061 ggaacaaagt cgctgagttc caaagctagc ctccggatag tgaaagacaa ggccatccag
     3121 ctctctgtcc aaccgttgtt cggcgtggag atgctgcgct tgtatgctga cagggacagc
     3181 cttgtgctgt tggacaggat gaacaaacgt tatgtaaaag agtccatcga ggatgttaaa
     3241 aaaatgtatc ctgtggggtt tgattataag acgttgcaat cgcttttaac caatcaagtg
     3301 tttgtttcgg gacaaacgga cgttatttat tccgattacg ataagttttc aaccggccaa
     3361 ctctccgata tgcattacct gttgaagtca gttgacaaga aatcgggcat cgagtatagc
     3421 tttaccatcg atggcaacga ccgcatcgca ttaacgcatt tgatagaacc gaaagatcat
     3481 tattcgatga actggaggta cgatgatttt gttatggaat cccataaggc tttccctcgc
     3541 aagatggatg ttgcgttggc ttcaaccagg cgaaaagtaa atgttggact ggagttttca
     3601 ggcatcacac ttgatgagga tttcgatctt caggtctcgg ttccggggag ttatacgcgg
     3661 gcgacccttt cggatattgt taaaatcctg acaaacaatt gatgaggaga gtttttacga
     3721 taggattgtt tctgacgctt gcgagtactc ttttctcgca aactccgcaa atcgatcagc
     3781 tccagaagca acagaagatg ttgttggaag agatcaaaag caccaacaaa ctcttcctcg
     3841 atgtaaaaaa agaaaccact accctccttc agcgcattaa ccttataaac aaacagattg
     3901 cttcccggaa agagatgatg acactgcaaa accaggaaat tgcagcgttg gagagggagc
     3961 aattgcggtt agaatcggaa ataaatcggc tgggtgaatc gttaaaacag aaacaggaga
     4021 gctacgcaaa agctgttcgt gtgatgcagg ccagccgaag taggcagaac gagttgtttt
     4081 ttgtcctttc cggaaaatcg ttgggtgaat cgttgcgcag gatgcaatac ctgcgtgatt
     4141 attctaaatg gcgcaaaagt caggccgaag agatcaagca acaaaatgta cagttaagcg
     4201 ctcgaaaaga tgagcttgcc aaagcccgga ccggcaaaca ggctgcgctg gatgccttga
     4261 aagcggaaca gctccggttg caggaagaag aacacgtccg tcagtctgaa atgaccgaag
     4321 cgcgaggaaa acaaagggag ttgcagagga tgctccagga taagcagcag caagcccgaa
     4381 aactagatgc ccagatcgaa aagttgattg ctgaagaggt tgcccgtcag gaaagggaag
     4441 ctgaagcccg tcgtttggcg gctgaagccg agagaaaacg aaaggccgaa gaggttgctg
     4501 cccgagtgcg ccgtgaagca gagaaaggaa gaaaaaccga ggaactggct agaaaagaac
     4561 cggataaaac cgaggacgta aaacctgccg tcaagcaacc tgaaccgaaa atagaatcgg
     4621 caacaacagc tgacgaaacg ttcaatcttt ctaagaattt tgtgtctaat cgcggaaatc
     4681 tgcctatgcc tgttactgga acggcatcca ttgtgggaag tttcggacag cgaaaacaca
     4741 gcgaatggaa cgttaccacc aatagtaatg gtattgatat tcaggcgcaa caaggcgcca
     4801 atatccgtgc ggtgtttgag ggtgaggtct cgaaagtgtt ctccgttccc ggttacaaca
     4861 cctgtgtgat tgtccgtcac ggagattatt acacatttta tggcaatatt tacgatctgt
     4921 ttgtaaaatc gggcgataaa ctaaagtctg gtcagtcact gggcagaatt tataccgatc
     4981 cggacaccgg tgtagcaacc atgcattttc agttgtggca aaaaaccacc aagctaaatc
     5041 cggcgccgtg gctgaaacga tagtacatag aagagactgg actgccgggt ctctacctaa
     5101 aatatcaaac atttaaatca tgaacataaa aatcagactc atcgtgatga actttctcca
     5161 atacgccatt tggggagcgt ggctaatttc gttaggtgca tatcttggtg gtggattgaa
     5221 gtttacgggt gtgcaaatcg ggagtttttt tgccactatg ggtattgcat cgctttttat
     5281 gcctggtgta atgggtgtga ttgccgaccg atggatcccg gcacaaaaat tactcggtat
     5341 ttctcatctg attgccggtt ttttcctttt tttggctgca ccgcaaacgg agtatgctcc
     5401 attatatacc tatgttttgc tgtcggttat gttttatatg cccactattg cattatctaa
     5461 ttcagtggca tacacatcgt tgacctcttg tggattggaa acggtg
//
LOCUS       DAOSDR010000221         5419 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrimonas sp. isolate AMR_MDS_1826
            NODE_5390_length_5419_cov_3.687360, whole genome shotgun sequence.
ACCESSION   DAOSDR010000221 DAOSDR010000000
VERSION     DAOSDR010000221.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863355
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrimonas sp. (wastewater metagenome)
  ORGANISM  Petrimonas sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Dysgonomonadaceae; Petrimonas.
REFERENCE   1  (bases 1 to 5419)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5419)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:10:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,720
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 35
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5419
                     /organism="Petrimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5390_length_5419_cov_3.687360"
                     /isolate="AMR_MDS_1826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2023866"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..704)
                     /locus_tag="PLI30_09110"
     CDS             complement(<1..704)
                     /locus_tag="PLI30_09110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019538597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK 4TM domain-containing
                     protein"
                     /protein_id="HOI79749.1"
                     /translation="MAKSTKRKRKKTTTATARKRRTAIISFLKNERVRFIFGILVAFL
                     GIFTLLAIISFFFTGAADQSKVLNKSFWELTQDKQLEVDNWTSTGGAFLAETLVNKWF
                     GLFSILIPFFVIYLGLRLMKVSDFSFIKVLLITAFGLVCGSITSAFIFGRLFPKSHIN
                     WGGAHGSQIERLLESSIGVPGMILLVLLFILIIVIVFRQSSMHKIQETLINSKPTFNG
                     NKESQPPIELEFDEKE"
     gene            complement(850..1068)
                     /locus_tag="PLI30_09115"
     CDS             complement(850..1068)
                     /locus_tag="PLI30_09115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI79750.1"
                     /translation="MKSIIGIILIAGAVILGYLGITNLQKSSKSVEILGMEITAEDNK
                     GKEIAYVEIGVAIITLIGGIYLLGQKKR"
     gene            complement(1264..1764)
                     /gene="tpx"
                     /locus_tag="PLI30_09120"
     CDS             complement(1264..1764)
                     /gene="tpx"
                     /locus_tag="PLI30_09120"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019538591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0016684 - oxidoreductase activity, acting
                     on peroxide as acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol peroxidase"
                     /protein_id="HOI79751.1"
                     /translation="MTKITFKGNPVNTGGNLPNVGSLAPDFSLVKTDLSELKLSDLKG
                     KNVVLNIFPSLDTGVCATSVRRFNKEASSLNNTTVLCISADLPFAAGRFCGAEVLENV
                     VTLSTFRDHSFADKYGVLMTDGPLHGLLARAVVVVNPEGRVVYTELVPEIAQEPDYES
                     AINSIV"
     gene            complement(1935..4754)
                     /gene="uvrA"
                     /locus_tag="PLI30_09125"
     CDS             complement(1935..4754)
                     /gene="uvrA"
                     /locus_tag="PLI30_09125"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009319425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HOI79752.1"
                     /translation="MTPETERIEVYGARVHNLKNIDVTIPRDSLTVITGLSGSGKSSL
                     AFDTVFAEGQRRYIETFSAYARGFLSKMERPDVDKITGLSPVISIEQKTTNRNPRSTV
                     GTTTEIYDFLRLLYARAGEAFSYITGEKMVKYTEEQILELILTRYAGKKIYILAPVIR
                     NRKGHYKELFEQIRKKGFISVRVDGEIREILPGMKLDRYKNHSIEVLVDKLAVSNKFE
                     DKLRSSVRTAMKQGSGLILIQDTVSGEVRHYSRELMCPTTGLSYNEPAPHNFSFNSPL
                     GACPKCKGIGTIDSIDTEKVIPDPSISIAKGGIVPLGKEKSNLFFWQVAAICEKYGVT
                     LQTPIRDIPQDAIDEIIYGTDERLKIKNESLGNSNYMVSYEGIAKYIDMQQDDDASAS
                     AQKWAGQFISTSVCPECNGQRLNREALHFKINGKNIAELSHMDIQQLHDWIVDAGDHV
                     SEKQQLIAEEINKEILSRLRFLIDVGLGYLSLSRASASLSGGESQRIRLATQIGSQLV
                     NVLYILDEPSIGLHQRDNHRLIDSLKKLRDSGNSVVVVEHDKDMMLASDYVVDMGPFA
                     GQKGGEVVFEGTPEEMLKKNTLTSNYLNGKIEIKIPGKRRKGNGKTLVVGGATGNNLK
                     NVTVQFPLGTLICVTGVSGSGKSTLINETLQPILSQKFYRSLKDPMPYKEVHGIKNVD
                     KVVSVDQSPIGRTPRSNPATYTGVFSDIRTLFSGMPEAKIRGYKPGRFSFNVKGGRCE
                     TCGGNGYKTLEMNFLPDVMVPCEGCHGKRYNRETLEVRFKGKSIADVLNMSINEAVDF
                     FENIPNILHKIKVLQEVGLGYIKLGQSSTTLSGGESQRVKLAAELARTDTGNTLYVLD
                     EPTTGLHFEDIRILLGVLNKLVDKGNTVIVIEHNLDIIKCADYIIDLGPEGGEKGGNV
                     LVAGTPEEIIKHKGSYTAKYLKEEL"
     gene            complement(4794..>5419)
                     /locus_tag="PLI30_09130"
     CDS             complement(4794..>5419)
                     /locus_tag="PLI30_09130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019538589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HOI79753.1"
                     /translation="ESSLDPRAVSPARASGLWQFMSGTAQQFGLEVSPEVDERYHIEK
                     STVAACRYLKDAYRKLGSWSAAALSYNGGQARITSEFTKQQTDKALDLWLVEETTRYY
                     FRMLAIKTLFENPTQYGFVLRSDQLYKPMEFKEVMVSSAIPSLASFAKENGITYAQLK
                     DFNSWLRDTKLTNKSGKNYTFLIPTRESLYYKKGEKIKIHDERWIAR"
BASE COUNT         1287 a         1263 c         1253 g         1616 t
ORIGIN      
        1 tcttcttttt cgtcaaactc gagttcaatg gggggttggc tctctttatt tccgttaaag
       61 gtgggtttgc tattgatgag tgtctcctgg atcttgtgca ttgagctttg tcggaagaca
      121 atcacgatga taaggatgaa caaaagtacg agaagaatca ttcccggtac cccgatgctg
      181 ctttcgagca ggcgctcgat ctgacttcca tgcgcacctc cccagttgat atggcttttg
      241 ggaaacagac gcccgaagat aaaggcactc gtgatggaac cgcaaaccag cccaaaggca
      301 gtgataagca gcacttttat aaagctgaag tcggaaacct tcatcagcct caatcctaaa
      361 taaatgacga aaaagggaat aagtatcgaa aataagccga accatttgtt taccagtgtc
      421 tcagctaaaa aagccccgcc ggtgctcgtc cagttatcta cttccagttg cttatcctga
      481 gtcagttccc agaaggattt attgagcact ttgctttgat ctgccgcccc ggtaaaaaag
      541 aaagagatga ttgccagcaa cgtgaatatt cccagaaaag caacgagaat gccgaaaatg
      601 aaacgaaccc tttcattttt taaaaaactg attatggcgg tcctccgctt tcttgcagtg
      661 gcagttgttg tctttttcct ttttcgtttt gttgattttg ccatatataa aagttctcat
      721 cgatttgcca ttaacccgat gaaaaacagg agttggatcg gtaaatttat tacaaaagta
      781 ataaaaagaa acttagatgg aaagctttat gggtttataa aaaaaagaaa tgaccggttt
      841 tgtgtgggat tatcttttct tctggccaag taagtagatt cccccaatca gggtaatgat
      901 ggcgactcca atttcgacat aggcgatctc cttgccttta ttgtcttcag cagtgatctc
      961 catgccgaga atttctaccg attttgaact cttttgcagg tttgtgatgc cgagataacc
     1021 gaggatgacg gctcccgcga taagaatgat tccgatgatt gatttcatgt ttgttttatt
     1081 attttttatt taagtttcgc atgtgttaag ataacagcta atcttctctt aatattccag
     1141 acatgcggaa gttcagtgaa tggcaatttt aagtgtttgt ttgtgttgtg tgtttctgaa
     1201 aagtgaaaag cccgcgaatc acttcgcaga cttttcttgt taacacaatc ttcatgaaac
     1261 aaactacact atcgagttga ttgcagattc ataatccggc tcctgtgcaa tctcgggaac
     1321 cagttcggta tatacaactc tcccttcggg attaacgact accacagcac gggccaataa
     1381 tccgtgaagc gggccgtcag tcatcagcac accgtacttg tcggcgaatg agtggtcgcg
     1441 aaaagtggaa agtgtaacca cgttctccaa tacttcagcg ccgcaaaaac ggcctgcagc
     1501 aaacggcagg tctgccgaaa tgcagagaac ggttgtatta tttaaagagc tggcttcttt
     1561 gttgaatctg cggacagaag tagcacaaac accagtgtcc aggctcggga aaatgtttaa
     1621 gactacgttc tttcctttca gatcggatag ttttaattcc gaaagatccg tttttaccag
     1681 cgaaaaatcg ggtgccagcg aacccacatt cggtaaattg cctccggtat ttaccggatt
     1741 tcctttaaat gtgatttttg tcatgttttt aattttattt ctttgggttt acataaaccc
     1801 actctctatt acactcttta attattcctc aaaaactata acaagacggt agttgaaaag
     1861 ttcaggaaaa cctgattttt aactttttat ttagaaacag ttttttattt tcaaaaacgg
     1921 ctaattcgct ttcatcacaa ctcttccttc aggtactttg ccgtatagct acccttgtgc
     1981 ttaattatct cctcgggtgt tcccgcgaca agcacatttc cgcccttttc cccgccctct
     2041 ggaccaagat cgatgatgta gtcggcacac ttgatgatgt cgagattgtg ttcgataaca
     2101 atcactgtat ttcccttgtc caccagttta ttgagtactc ccaataaaat cctgatgtcc
     2161 tcgaaatgta atcctgtagt gggttcatcc agtacataaa gggtatttcc ggtgtcggtc
     2221 cgggccagtt cggccgcaag ttttacccgt tggctttctc cgcccgagag ggttgtggac
     2281 gattgtccga gcttgatata tcccagtccc acctcctgaa gcacctttat cttatgcagg
     2341 atgttgggaa tgttttcgaa gaaatctacc gcctcgttga tcgacatgtt gagtacgtcg
     2401 gcaatggatt tccctttaaa ccgtacttcc agtgtttccc ggttgtatct tttcccatgg
     2461 catccttcac agggaaccat tacatcgggt aggaaattca tttccagtgt tttatatcca
     2521 ttccctccac aggtttcgca acgtcctcct tttacgttga atgagaatcg tccgggtttg
     2581 taaccgcgga tttttgcttc gggcattccg gaaaacaggg tgcggatatc gctgaacaca
     2641 cccgtgtaag ttgcaggatt cgagcgggga gtcctcccga tgggagactg gtccacacta
     2701 actactttgt ctacattttt tatgccgtgc acctccttgt atggcatcgg gtctttcagg
     2761 gaacggtaga atttctggct caggataggt tgcaacgttt cgttgataag cgtagatttg
     2821 ccgctccccg acactcctgt tacgcatatg agtgttccca acggaaattg tacggtaacg
     2881 tttttcaggt tgttgcctgt tgctcctccc acaacgagtg ttttgccgtt ccctttgcgc
     2941 cgtttcccgg gaatcttgat ttctattttt ccgttcaggt aattggaggt tagcgtattc
     3001 ttcttgagca tctcttcggg agtgccttca aaaaccactt cgcctccttt ttgtccggca
     3061 aacggaccca tgtctaccac atagtcggat gcaagcatca tgtctttatc gtgctcgacc
     3121 accactacgg agttgccgga atcgcgcagt tttttcaacg aatcgatcag ccggtggtta
     3181 tcccgttgat gcaatccgat actgggttca tccaggatat acaatacgtt taccaattgc
     3241 gaaccgatct gcgtagccag ccggatacgc tggctttccc cgcccgagag cgatgccgat
     3301 gcgcgggaaa gggaaaggta acccagtccg acatcaataa gaaagcgcaa ccgcgaaaga
     3361 atctccttgt tgatctcttc ggcaatgagc tgctgttttt ctgaaacgtg atcaccggca
     3421 tctacaatcc agtcgtgcag ctgttggata tccatgtgcg acaactcagc aatgttcttg
     3481 ccgttaattt tgaagtgaag tgcttcccgg ttgagccgtt gcccgttaca ctccggacaa
     3541 accgacgtcg atatgaattg tcccgcccat ttttgcgcag aagccgatgc gtcatcatcc
     3601 tgttgcatat cgatgtactt ggcaattcct tcgtaactta ccatgtagtt ggagttaccc
     3661 aatgactcgt ttttgatttt caggcgttcg tcggtaccgt agataatttc gtcgatagca
     3721 tcttgcggga tatcccggat gggcgtctgc agcgtaactc cgtatttttc gcaaatggca
     3781 gccacttgcc agaaaaacag gttgcttttt tcttttccta gcggcacgat tccgcctttg
     3841 gcaatactta tggagggatc ggggatgacc ttttccgtat cgatggagtc gatggtccct
     3901 atccctttgc atttggggca tgcgcctaat ggtgagttga acgagaaatt gtgcggagcg
     3961 ggttcgttgt aggataatcc ggtggtaggg cacattaact cgcggctgta gtggcgcacc
     4021 tcgccggaca cggtgtcctg gatgagtatc agccccgaac cctgcttcat ggcagtcctg
     4081 acactcgatc tgagcttatc ttcaaacttg ttggacacgg ctagtttgtc caccagtact
     4141 tcgatgctgt ggttcttgta cctgtccagc ttcatccccg gcagaatttc ccggatctcg
     4201 ccgtccacgc ggacactgat aaatcccttt ttccggattt gctcgaacag ctctttgtaa
     4261 tgtcctttcc tgtttcgtat aacgggtgcg aggatgtata ttttttttcc ggcataccgg
     4321 gttaagatca gttccagaat ttgctcttcg gtatatttta ccattttctc gcccgtaatg
     4381 tacgagaacg cttctccggc acgggcataa agcaaccgta ggaagtcgta aatttctgtg
     4441 gtggtgccaa cggtagaacg tggatttcgg ttggtggttt tttgttcgat ggaaatgacg
     4501 gggcttaatc cggtaatttt atccacatcg ggacgttcca ttttgcttaa aaatccacgg
     4561 gcataggccg aaaatgtttc tatataacgc cgttgccctt cggcaaaaac ggtgtcgaag
     4621 gccagggacg acttgccgct tccgctcaat ccggtaataa ccgtaagtga atcgcgcggg
     4681 atggttacgt caatattttt gaggttgtgt acgcgtgcgc catacacttc aatacgttct
     4741 gtctcaggag tcattttgtt gaaaatacga acctgcaaag gtagtgaaaa agatcatctt
     4801 gcgatccatc gttcgtcgtg aatttttatt ttctcccctt ttttgtagta gaggctttcc
     4861 cgggtgggaa taaggaaagt atagtttttt cctgatttat tggtgagctt ggtatcgcgg
     4921 agccacgaat tgaaatcctt tagttgggca taggtgatgc cgttctcttt ggcaaacgat
     4981 gccaggcttg gaatggccga ggaaaccatg acttccttga actccatggg tttatacagt
     5041 tgatcgctac gaaggacaaa tccgtattgt gtcgggtttt caaacagggt tttaatagcc
     5101 agcatacgga aataataacg ggttgtttct tccaccagcc ataaatcaag cgctttgtcg
     5161 gtttgctgct tggtgaactc gctggtgatc cttgcttgtc ctccgttgta ggacaaggcg
     5221 gcagcgctcc acgaaccgag cttccggtac gcgtctttga ggtaccggca agcggcgacg
     5281 gttgatttct cgatgtggta acgctcatcc acttcgggcg atacctcaag tccgaattgt
     5341 tgagctgtac ccgacataaa ctgccacaat ccgctggcac gggccggtga gaccgcccgg
     5401 gggtcgaggc tgctttcaa
//
LOCUS       DAOSDR010000222         5374 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrimonas sp. isolate AMR_MDS_1826
            NODE_5433_length_5374_cov_3.567588, whole genome shotgun sequence.
ACCESSION   DAOSDR010000222 DAOSDR010000000
VERSION     DAOSDR010000222.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863355
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrimonas sp. (wastewater metagenome)
  ORGANISM  Petrimonas sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Dysgonomonadaceae; Petrimonas.
REFERENCE   1  (bases 1 to 5374)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5374)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:10:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,720
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 35
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5374
                     /organism="Petrimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5433_length_5374_cov_3.567588"
                     /isolate="AMR_MDS_1826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2023866"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..440
                     /locus_tag="PLI30_09135"
     CDS             <1..440
                     /locus_tag="PLI30_09135"
                     /inference="COORDINATES: protein motif:HMM:NF024247.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HOI79754.1"
                     /translation="EYGWTAVMQPTLSFERGYCRTDCTVCSDVCPTDAITKLSKGDKT
                     KVAMGHAVYVRENCIVVKDDVNCDGCWRICPTDAITREYRDSGASYLSPAGQPDANTV
                     DLRLVPVVNKDKCIGCGACEYICPARPLSAIFVEGYKKHLALN"
     gene            464..1003
                     /locus_tag="PLI30_09140"
     CDS             464..1003
                     /locus_tag="PLI30_09140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006842332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin family protein"
                     /protein_id="HOI79755.1"
                     /translation="MKKRQLFLSLTVIGIMSAFFSCSTLPKGAVAVRPFDKEKYLGKW
                     YEIARLDFKYEKDLDNTTAEYSLNADGTIKVDNKGYHTKKEEWKQAVGKAKFVATEDV
                     AMLKVSFFGPFYSGYNVIAIDPDYTYALVAGASLKYLWILSRETSIPDDIKSKYLKIA
                     QDIGYNVADLLWIEHNKRP"
     gene            complement(1011..2012)
                     /locus_tag="PLI30_09145"
     CDS             complement(1011..2012)
                     /locus_tag="PLI30_09145"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004313602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HOI79756.1"
                     /translation="MKNLNAVITGVAAAVPDYILTNEELSRMVDTSDEWIMTRVGIKE
                     RRILKGEEQGISVLGTKAVKELLEKTHTKPEEVDGVIFATTTPDHPFPSAASIVAENN
                     GIKNAFCFDMEVACSGFVYGLEVCNGFIKTGKYKKIILIAGDKMSAVTNYTDRATCPL
                     FGDAAGAVMIEPTEENLGIVDTILRSDGAGYAPLQMKAGGSKYPATHETVDNNEHTVY
                     QEGKVVFKYAVSNMAQVSLDIMERNNLTHEDLDWFVPHQANIRIIDAAVARAGVAPEK
                     VMINIERYGNTSAGTIPLCLWEWERNLRKGDNIILTAFGAGFSWGSVYLKWGYDGNK"
     gene            complement(2087..2272)
                     /gene="rpmF"
                     /locus_tag="PLI30_09150"
     CDS             complement(2087..2272)
                     /gene="rpmF"
                     /locus_tag="PLI30_09150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002562387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HOI79757.1"
                     /translation="MAHPKRRQSSARQGKRRSHDHAKMPTMAICPNCGAWHIYHTVCG
                     ECGYYRGKLAIEKEVAV"
     gene            complement(2308..2886)
                     /locus_tag="PLI30_09155"
     CDS             complement(2308..2886)
                     /locus_tag="PLI30_09155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019539021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HOI79758.1"
                     /translation="MAKFSLYNIPLKNLSQGKHTYEYELDRKFFEAIDGDEVKKGNVN
                     VVLTVKKTSSTYEFNFDLKGIVQVPCNRCLDDLDQEIDSQNRLVVKLGKEYSEESDEV
                     VIIPEDEGEINIAWFLYEFVALNIPIKHVHEPGKCNKTMSTKLRKHRAVSRDDEDDGG
                     DIPDEETEFADEEEITENIDPRWEGLKGLNLE"
     gene            complement(2987..4078)
                     /locus_tag="PLI30_09160"
     CDS             complement(2987..4078)
                     /locus_tag="PLI30_09160"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005854705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside phosphotransferase family
                     protein"
                     /protein_id="HOI79759.1"
                     /translation="MSKLKEIVAQFVGDSEEIDVKPLGAGHINDSYKVKAGEKEYVLQ
                     RINHSIFKNVPKLQSNILRVTMHIRQKLMDAGVNDIDRKVLTLVPTPGGKLFYQDAEG
                     SYWRMMDFIRDSKSYDEINPELAYRAGLAFGEFQKMLADLPGDPLFETIPNFHNIESR
                     LETFRDSVRANKVGRLVEVQDLVSELEARADEMCKAERMHREGKLIKRTNHCDTKVNN
                     ILFDKNDQVLCVVGLDTVMPGYVLSDFGDFIRTGANKGAEDDQDLDNVSLDLAIFEGY
                     TKGYLENAASFLTEVEIENLAFGAKLLTYMQTVRFLTDYIDGDTYYKIAYGNHNLVRS
                     KAQFKFLQSLEQNFDRMQEIVLSTAEKYR"
     gene            complement(4129..4776)
                     /locus_tag="PLI30_09165"
     CDS             complement(4129..4776)
                     /locus_tag="PLI30_09165"
                     /inference="COORDINATES: protein motif:HMM:NF027338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0016052 - carbohydrate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate-binding family 9-like protein"
                     /protein_id="HOI79760.1"
                     /translation="MKTLKVPKQHISQCETIFDCINLLREAGVVAKIDQVNWKKEFPK
                     SLPVTVRVAHDGEKIYLCFEVVGEKIRAVNTEDFGSVWEDSCVEFFMQREGEAVYRNF
                     ECNILGALLAAKHETRQIAEKLTEHMSSISRFSTIRHRYENDVQVSDWMMFLIIPRQA
                     MGFHADESLSGKKIRANFYKCGDKTPETHFISWSPIDLPSPDFHAPQFFGLLEME"
     gene            complement(4844..>5374)
                     /locus_tag="PLI30_09170"
     CDS             complement(4844..>5374)
                     /locus_tag="PLI30_09170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011108785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C-terminal helicase domain-containing protein"
                     /protein_id="HOI79761.1"
                     /translation="ANHPALVDCGFSGQSGKFEQVIDTVETLFQEGHKVLVFSSFVKH
                     LQLFADYFDARRWKYAWLTGQTIRREIEITEFNSNPEVRAFFISLKAGGTGLNLTAAD
                     YVFILDPWWNPAAEMQAVSRAHRIGQERKVTLYRYITLGTVEEKIRRLQQYKSALSDA
                     LVRPQLTMEEAEELLR"
BASE COUNT         1396 a         1296 c         1092 g         1590 t
ORIGIN      
        1 atgaatatgg atggacagcg gtcatgcagc ctaccctctc tttcgaacgc ggttattgcc
       61 gtaccgactg cactgtttgt tcagatgttt gccctaccga cgccatcaca aaactttcaa
      121 aaggagataa gaccaaagtg gcgatggggc acgccgttta tgtacgtgag aactgtatcg
      181 tggtgaagga tgatgtgaac tgcgacgggt gctggcgtat ctgccctacc gatgccatta
      241 cccgtgagta ccgcgatagc ggcgcaagct atctttcgcc ggccggtcag ccggacgcaa
      301 atacggttga tttgcggctt gtacccgtag tgaacaaaga caagtgcatc ggatgcggtg
      361 cctgcgaata tatttgcccg gcaaggccgc tatctgctat atttgtggaa gggtataaaa
      421 aacatttggc gttgaattga caaggccaca gctttttaaa tcaatgaaaa aaagacaatt
      481 attcttatct ctaactgtaa ttggcattat gtccgcattt ttttcctgtt ccaccctccc
      541 taagggagct gttgccgtca gaccttttga caaagagaaa tatcttggga aatggtacga
      601 gattgccagg ctggatttta aatacgagaa agacctggac aacaccactg ctgaatattc
      661 tttgaatgcc gacggaacca ttaaagttga caataaaggg taccacacaa aaaaagaaga
      721 atggaaacaa gcggtgggaa aagcaaaatt tgtagcaacc gaggatgtgg caatgctgaa
      781 ggtgtcgttc tttggcccat tttactcggg ttacaacgtc attgccattg atccggacta
      841 tacctacgct ttggttgccg gagctagttt aaagtatctg tggatcctgt cccgggaaac
      901 aagcatcccg gatgatataa aaagcaaata cttaaagata gctcaggata tcgggtataa
      961 tgtagcggac cttttgtgga ttgaacacaa taaacgtccg tgaatcattt ctacttgttg
     1021 ccatcgtacc cccattttag gtagacactt ccccaagaga agcctgctcc gaacgcggtg
     1081 aggataatgt tgtcgccctt gcggaggttg cgctcccatt cccataagca aagcggaatg
     1141 gttccggcac tggtattgcc ataacgttcg atatttatca ttactttttc gggggcaact
     1201 ccggcgcgag ccacggcggc atcgataatg cggatattgg cctggtgagg cacaaaccaa
     1261 tccagatctt catgagtgag gttattacgc tccataatgt cgagcgagac ctgtgccatg
     1321 ttggatacgg catacttaaa tacgactttt ccttcctgat atacggtatg ttcattgtta
     1381 tccactgttt cgtgagtggc gggatactta cttccaccgg ctttcatttg tagcggggcg
     1441 taacctgcac catcggaacg aagaattgta tcgacaatac ccaagttttc ttccgttggt
     1501 tcaatcataa cggctccggc agcatcccca aaaaggggac aagtagccct gtcggtatag
     1561 tttgttacag ccgacatttt atcaccggca atcaggataa tttttttgta ttttccggtc
     1621 ttgataaatc cgttacatac ttcaaggcca tacacaaacc ccgagcaagc cacctccata
     1681 tcgaaacaaa aagcgttttt tatcccgttg ttttcagcaa cgatagatgc cgcggatggg
     1741 aagggatggt caggcgttgt tgtagcaaaa atcacaccat caacctcttc gggtttggtg
     1801 tgggttttct caagcagttc ctttactgct tttgtcccta aaacagatat tccctgttct
     1861 tctcctttta aaattcttct ctctttaatt cccacacgag tcatgatcca ctcgtcggaa
     1921 gtatctacca ttctcgatag ctcctcattg gtaagtatat aatcggggac agctgctgca
     1981 actcctgtaa taaccgcgtt taagttcttc attatcctga ttttattgta aaaaaacccc
     2041 gaaaaacaag gttattcagg gttttgctta ttacgaatga atgagtttat accgcaactt
     2101 ctttttcaat tgccaatttt cctctgtagt acccgcactc accacaaaca gtgtgataga
     2161 tatgccatgc tccgcaatta gggcagatgg ccatagttgg cattttggca tggtcgtgcg
     2221 atcttctttt tccttgtctt gcagacgact gtcttcgttt tggatgtgcc attttgtata
     2281 tttatttatt aacgtttata ttttaagtta ttccaaattc aatcctttta ggccttccca
     2341 tcttggatcg atgttttctg ttatttcttc ttcatcggca aactcagttt cttcgtccgg
     2401 aatatcacca ccgtcatctt cgtcatctct gcttactgcc cggtgcttac gaagtttggt
     2461 ggacatggtc ttgttgcatt tacccggttc gtgaacatgc tttatcggaa tattcaacgc
     2521 cacaaactca tacagaaacc acgctatatt gatctcccct tcatcttcag gaataataac
     2581 cacctcatca ctctcttccg aatattcttt cccgagtttc accactaagc ggttttgaga
     2641 atcaatctcc tggtccaggt catccagaca acggttacaa ggcacttgaa caataccttt
     2701 taaatcgaaa ttaaactcat acgtggatga agtttttttg acagtcaaca ccacattgac
     2761 attgcctttt tttacctcat caccgtcaat cgcctcaaaa aactttctat ctaactcata
     2821 ctcataagta tgttttccct gagaaagatt ctttaaagga atattgtata agctgaactt
     2881 tgccactttt tttacctaaa aataaagcgg tgcaaagata gtgatttttt cttcacactg
     2941 ctcttttttt acattattta ttttcccgta taaaccgtgc tgttttttat ctatattttt
     3001 ccgccgtact taacacaatc tcctgcatcc tatcgaaatt ctgttcaagg ctctgtaaaa
     3061 acttaaactg agcttttgaa cgcaccaggt tgtggtttcc gtaagcaatt ttgtaataag
     3121 tatcgccatc gatatagtcg gtcagaaaac gtacagtttg catataggtc aataacttgg
     3181 cgccgaaagc aaggttttct atttccacct cagtaaggaa agaagcagca ttctccagat
     3241 aacctttagt atatccttca aagatggcca gatcaagcga aacattgtct aaatcctgat
     3301 catcttcagc acctttattt gcccctgtac ggataaaatc tccaaaatcg gacaatacat
     3361 agcccggcat aaccgtatct aaacccacca cacacaacac ctggtcgttc ttatcgaaaa
     3421 ggatattgtt cactttggta tcacaatggt tggtacgctt gatcaatttc ccttcccggt
     3481 gcattctttc tgctttgcac atttcatcgg cacgggcctc cagttcacta acaagatcct
     3541 gtacctcgac caaacggccc actttatttg ctctaaccga atccctgaac gtttccagtc
     3601 tcgattcaat gttgtgaaaa ttcggaatgg tttcaaacaa tggatcaccc ggcaggtcag
     3661 ccagcatttt ctgaaattcg ccaaaagcga gtccggccct gtaagccaat tcaggattta
     3721 tctcatcgta acttttgcta tcccggataa aatccatcat ccgccagtaa gaaccctctg
     3781 catcttggta aaaaagtttt ccgccgggtg tgggaacgag agtcaacacc ttacggtcga
     3841 tatcgttcac cccggcatcc atcagtttct ggcggatatg catcgttacg cgcagaatat
     3901 tgctttgcag tttcggtacg ttcttaaaaa tagaatgatt aatacgctgt agcacatact
     3961 ctttctcgcc agctttcacc ttgtatgaat cgttaatgtg acctgctcct aacggtttta
     4021 cgtcgatctc ttcactgtcg ccgacaaact gggctacgat ctctttcaat ttactaattt
     4081 cctgcatatg tatttaacta tgtttgtgtt atctgtacga gtttcggctc actccatttc
     4141 caacagtcca aagaactgtg gagcgtgaaa atcgggtgaa ggcaaatcga tggggctcca
     4201 actgatgaaa tgtgtttcgg gggtcttatc accgcactta tagaaatttg cccggatctt
     4261 ttttcccgat aaactctcat cggcatggaa acccattgcc tgtcggggaa ttatcaggaa
     4321 catcatccag tcgctgacct gtacatcatt ttcatacctg tgtctgatcg tagaaaaacg
     4381 ggagatcgag gacatatgct ccgtcagttt ttccgctatt tgccgggttt cgtgctttgc
     4441 tgcaagtaac gctcccaata tattacactc aaaattccta taaacggctt cgccttcccg
     4501 ctgcataaaa aactctacac aactgtcttc ccagaccgaa ccgaaatcct ctgtattcac
     4561 tgcacgtatt ttttctccta ccacttcaaa acagaggtaa attttttctc catcgtgagc
     4621 aacacgcacc gtaacaggaa gtgacttggg aaattctttt ttccagttca cctggtcaat
     4681 tttagcgact acgccggctt cgcgcaataa atttatacaa tcaaaaatag tttcgcattg
     4741 cgaaatgtgt tgtttgggta ctttgagagt tttcatcagc aattattatt tttttcaaaa
     4801 gtacctgttt tccccggatt taacaaaaat gccgcccact tttttaccgg agaagctctt
     4861 ccgcctcctc catcgtgagt tgcgggcgaa caagcgcatc ggaaagggcg gacttatatt
     4921 gttgtaaacg gcggattttt tcttccaccg tgcccagtgt tatataccgg taaagcgtca
     4981 ccttccgttc ctgcccgata cggtgggcac ggctgacggc ctgcatctcg gctgccgggt
     5041 tccaccaggg gtcgagaata aatacgtaat cggcggcggt gaggttcagt cccgttcccc
     5101 cggctttaag cgaaataaaa aaagcacgca cttccggatt ggagttgaac tccgttattt
     5161 cgatctcacg ccggatagtc tgtcccgtga gccaggcgta tttccaccgg cgggcatcga
     5221 aataatcggc aaacagttgc aaatgtttca caaacgatga gaacaccaac accttatgcc
     5281 cttcttgaaa aagggtctca accgtgtcga tgacctgttc gaacttgccc gattgtcccg
     5341 aaaaaccgca gtcgacaagc gccgggtgat tggc
//
LOCUS       DAOSDR010000223         5346 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrimonas sp. isolate AMR_MDS_1826
            NODE_5464_length_5346_cov_4.011907, whole genome shotgun sequence.
ACCESSION   DAOSDR010000223 DAOSDR010000000
VERSION     DAOSDR010000223.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863355
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrimonas sp. (wastewater metagenome)
  ORGANISM  Petrimonas sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Dysgonomonadaceae; Petrimonas.
REFERENCE   1  (bases 1 to 5346)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5346)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:10:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,720
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 35
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5346
                     /organism="Petrimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5464_length_5346_cov_4.011907"
                     /isolate="AMR_MDS_1826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2023866"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..416
                     /locus_tag="PLI30_09175"
     CDS             <1..416
                     /locus_tag="PLI30_09175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006841863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HOI79762.1"
                     /translation="NTSYVESASGVAAGGRTGLTSVSTTLMFVLALFFAPIFLMVPAA
                     ATAPALIIIGLFMMSSVADIDFNEISEGLPAFVTIIFMPFTYSIANGIIFGMLSFTLV
                     KLLSGKVKDVSITMYVLTLFFLIKIILDASNAFVH"
     gene            519..1085
                     /gene="efp"
                     /locus_tag="PLI30_09180"
     CDS             519..1085
                     /gene="efp"
                     /locus_tag="PLI30_09180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004585684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HOI79763.1"
                     /translation="MATTADFRNGMCIDLEGQYYFIVEFLHVKPGKGAAFVRTKLKNV
                     TTGRVLEKTFNSGVRVDEVRIERRPFQYLYQDDMGYNFMNTETFEQIPIPAEQIEGVQ
                     FLKEGDLVEVQIHSESGTILTAEMPTHVVLQVTYTEPGIKGDTATNTLKPATVETGAE
                     VRVPLFINEGEKIKVDTRTGTYVERVKE"
     gene            1185..1874
                     /gene="radC"
                     /locus_tag="PLI30_09185"
     CDS             1185..1874
                     /gene="radC"
                     /locus_tag="PLI30_09185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019539408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HOI79764.1"
                     /translation="MKKISIKEWAEEDRPREKMMLKGVSALSDSELLAILIGSGNDKE
                     SAVELCKRILQKAGNNLNKLGRFSVNDLVTNFRGIGPAKAITIIAALELGRRRKSEGA
                     VNRKKITSSDDAYAVFYPVLVDLNHEETWALLLDRSNKVVATLQVSRGGISGTVVDIR
                     LILREAISHYASSIVLGHNHPSGNCTPSAQDTAISKKLKEAARWMDISLLDHIIVCGE
                     TYYSFADNGMI"
     gene            1984..2301
                     /locus_tag="PLI30_09190"
     CDS             1984..2301
                     /locus_tag="PLI30_09190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008146258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly protein"
                     /protein_id="HOI79765.1"
                     /translation="MNDELQKLQDKIVVLLRTVYDPEIPVNIYDLGLIYDVDIDDTNN
                     VTIEMTFTSPSCPAADFILMDVQMKIESIPEVNSVDLNLTFDPPWDKSMMSEEALLEL
                     GFM"
     gene            2305..3042
                     /locus_tag="PLI30_09195"
     CDS             2305..3042
                     /locus_tag="PLI30_09195"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007558082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008758 - UDP-2,3-diacylglucosamine
                     hydrolase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HOI79766.1"
                     /translation="MNKKVYFVSDAHLGSKMHADSVETERKLCRWLDFVKEDAQAIYL
                     LGDIFDYWFEYKTVVPRGFTRLLGKLSEITDSGVVVYFFIGNHDIWLTDYLQNECGLI
                     VHRKPFVTELSGKKFFIAHGDGLADKSASFRILRRIFHSTFLRRCFAAIHPRWTVGLA
                     HAWSNKSRESGGVQDFLGEDNEYLIQFAKQEIIRTPDIDYFVFGHRHLLLDLPLNETS
                     RVINIGDWIQHFSYGVFDGKEMELKKF"
     gene            complement(3043..5298)
                     /locus_tag="PLI30_09200"
     CDS             complement(3043..5298)
                     /locus_tag="PLI30_09200"
                     /inference="COORDINATES: protein motif:HMM:NF036578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-rhamnosidase C-terminal
                     domain-containing protein"
                     /protein_id="HOI79767.1"
                     /translation="MRKQGCLLVYFALVVFCTYAQNKPTGLMTDLLKKTGEVFINGYP
                     STLDHEEIDSAIEPVQTAKILSEYPSFSWIVPNKGKNTLQSGYRIILSDSLNLIQKGE
                     GNVWDSGTVNSGRSVSVAYRGRSLQPRKVYYWRVKAITDNSEESDWSDIKSFRTGDEL
                     KPYQSSSEVLVKSVEHPVLCSTRDDNWFFDFGKASFGQLLVRLTSETGNDTVIVNLGE
                     KVRDGRVDSRPGGTIRYQSYSLALLKGTHLYRIKVHKDKRNTLEVAVKMPAYIGEVVP
                     FRYAEVLHYGNKLDKEDVIRESVHYPFDDSTSFFRCDNDTLNQIWEMSKYTMKATSFT
                     GVYVDGDRERIPYEADILINQLSHYSVDREYSIARKSLEYILKKPTWPTEWILQAILI
                     AWYDYMYTGDARSLEKNYPLLKNRSLMQLKTSKGLISTTTGLQTSAFLQSINFNKPIQ
                     DIVDWPGEERDGFVFCDHNAVVNAFYYEALKIMEQIARVLNKQEDRLFYAKAHQEVYK
                     LFNDTFFDPKRKLYTDGDTTSHTSLHANMFAFVFGLVPQQHSQGVQDFIKSRGMACSV
                     YGAQFLMDALYNGGNGAYAEELLASTGERSWYNMIRSGSTVALEAWDIKYKPNLDWNH
                     AWGAVPANTIARYVVGIKPSAPGFESIEIKPHVYSLTSVESAVPTIRGNILFTYKTIN
                     NDEYELSVEIPPNTQAELYLPIKSGKRVREVFLDGKKITFAKGKKEPEHLYVGRITSG
                     KQVYRVMLSNR"
BASE COUNT         1516 a         1192 c         1147 g         1491 t
ORIGIN      
        1 cgaatacctc atacgtggaa agtgcttcgg gggtcgcagc cggtggaagg acaggactta
       61 ccagtgtgag cactacacta atgtttgtac tggccctttt ctttgccccg atattcttaa
      121 tggtgccagc tgcagcaaca gctccggcac tgatcattat tgggctattt atgatgagtt
      181 cggttgctga tatcgacttc aacgaaataa gcgaaggact tccggcattc gtcaccatca
      241 tatttatgcc gttcacatac agcattgcaa acggaattat tttcggaatg ttgagtttca
      301 cccttgttaa actcttatcg ggaaaagtaa aagacgtaag cattaccatg tatgtactga
      361 cgcttttttt cctgattaaa attattcttg acgcgtcaaa tgcatttgtt cactaatggc
      421 tcaaaaaacg cactcaaccc gttaatttat ttgctgttct ggaataaaac cattactttt
      481 gcggcctaat ccaaatataa ctcagacaaa tacaatttat ggcaacaaca gctgatttca
      541 gaaacggaat gtgcatcgat ttggaaggac aatattattt catcgtagaa tttcttcacg
      601 tgaaacccgg aaaaggtgca gctttcgtac gtacaaaact taaaaacgta acaacgggac
      661 gtgttctcga aaagactttc aactcgggtg tcagggtgga tgaggttcgc atagaacgcc
      721 gtccgttcca atacctttat caggacgata tgggatataa cttcatgaac accgaaactt
      781 tcgaacagat acctatccct gccgaacaga ttgagggagt gcagttcctg aaagagggcg
      841 accttgtgga agttcaaatc cattccgaaa gcggtaccat cttaacagca gaaatgccta
      901 cacacgtagt actgcaggtt acgtataccg aacccggtat caagggcgat acggctacta
      961 acacattgaa gccggccacc gttgaaaccg gagcggaagt ccgagttccc ctgttcatca
     1021 acgaaggtga aaaaattaag gtggatacac gcacgggaac atatgttgaa cgtgtgaagg
     1081 aataacacct gctattggag aaatttacca gcaagaagaa agacatcgta ttttacggtg
     1141 tctttttttg tgttatcttt gcgattgacc gagaattgaa aggaatgaaa aaaatcagca
     1201 taaaagagtg ggccgaagag gatcgtccgc gtgaaaaaat gatgctgaag ggtgtttcgg
     1261 cactgtcgga ttccgagtta ctagctattc ttatcggttc aggcaacgac aaggagagcg
     1321 cagtggaact atgcaaaaga attctgcaga aagccggtaa taacctcaac aaattaggcc
     1381 gtttcagcgt taacgatctg gtgactaatt tccgtggtat cggaccggca aaagcaatta
     1441 ctataattgc tgctctggag ctgggtagaa gaaggaaatc ggaaggagcg gttaacagaa
     1501 aaaaaatcac ctcttccgat gatgcctatg ccgttttcta tcctgtgctg gtagatttaa
     1561 atcacgaaga gacgtgggca ttgcttctcg accgatccaa taaagtggtt gccactttgc
     1621 aggtcagccg tggtggtata tcgggaacag tagttgacat acggttaatc ctccgcgaag
     1681 caatcagtca ttatgcaagc agtatcgtgt tggggcataa ccacccgtcg gggaactgta
     1741 cgcctagcgc acaggatacc gcaatctcaa aaaaactgaa ggaggcagcc cgatggatgg
     1801 atatttcttt acttgatcat ataatcgtat gtggtgaaac gtattacagt tttgccgata
     1861 atggcatgat ataagaagat tgaataagat agataacagc ttctcaatct cgttcaatct
     1921 tcttcaagct aaatcaatct acaattttaa taaacagttc aacgcacttg aaaaaataaa
     1981 aaaatgaacg acgaattaca aaaacttcaa gataaaatag tggttttgct ccgcacggtg
     2041 tacgaccccg aaattccggt aaatatctac gacctgggat taatatatga cgtggacatt
     2101 gacgatacca acaacgtaac cattgaaatg acctttactt ctcccagttg tcccgctgcc
     2161 gacttcatcc tgatggatgt acagatgaaa atagagtcca taccggaagt gaacagcgta
     2221 gaccttaacc ttacgtttga tccaccatgg gataaatcga tgatgagcga agaagctttg
     2281 ctggaacttg ggtttatgta aaaaatgaac aaaaaagtat atttcgtttc cgacgctcat
     2341 ttgggttcaa aaatgcacgc cgattccgtt gaaacggagc ggaaattgtg ccgttggctc
     2401 gattttgtaa aggaagatgc gcaagccatt tacctgctgg gcgatatctt cgattattgg
     2461 ttcgaatata aaaccgttgt acccagagga tttacgcgtc tgctgggtaa actgtcggaa
     2521 attaccgact caggggtggt tgtgtatttt ttcattggca atcacgacat ctggctcacc
     2581 gattatctcc agaacgaatg cgggttaatt gtccaccgca aaccgtttgt cacggaactt
     2641 tccggcaaga aattttttat cgctcacggc gacggattgg ccgataaatc ggcttcgttc
     2701 aggatacttc gtagaatttt tcacagtacg ttcttgcgac gctgttttgc ggctatccat
     2761 cctcgatgga ccgtgggcct ggcacatgca tggtcgaata aaagccggga atcaggcggt
     2821 gtacaggatt ttttaggtga ggacaacgaa taccttatcc agtttgcaaa gcaggaaatt
     2881 atccgaactc cggatattga ctattttgtt ttcggacaca ggcacctctt attggattta
     2941 ccgctgaacg aaacgtcgcg cgttatcaat atcggcgact ggatacagca tttctcgtac
     3001 ggtgtctttg acggaaagga aatggaactg aaaaagtttt aattacctgt tgctcaacat
     3061 tacccgatac acctgttttc ccgaagttat tcttccaaca tacaggtgtt ccggctcctt
     3121 tttcccttta gcaaaagtaa tttttttacc gtcgagaaaa acctctctga cccgtttacc
     3181 ggatttaatc ggtagataga gttctgcttg ggtatttggc ggaatctcta ccgacaactc
     3241 atattcatca ttattgatcg tcttgtaagt aaataaaatg tttcctctaa tggtgggaac
     3301 agcagattca accgaagtaa ggctataaac atgcggttta atttcaatgc tctcgaaccc
     3361 tggagcagat ggtttaatac caaccacgta tcgggcaatc gtgtttgcag gaaccgctcc
     3421 ccaagcatga ttccagtcca ggttaggttt atatttaatg tcccaggctt ccagcgctac
     3481 tgttgagcct gatcgtatca tgttgtacca gctcctctca ccggtcgatg ccagcaattc
     3541 ctccgcgtac gccccattac caccgttata caaagcatcc atcaggaact gagccccgta
     3601 aacgctgcac gccattcctc tggacttgat aaaatcctgc actccctgag aatgttgttg
     3661 aggaactaac ccaaaaacaa aggcaaacat attggcatgc aatgatgtat ggctggtggt
     3721 atcgccatct gtatacagtt tccgtttagg atcaaaaaaa gtgtcgttaa atagtttata
     3781 aacctcttga tgtgctttgg cataaaacaa cctgtcctcc tgtttattca atactcgggc
     3841 aatctgctcc attattttca gggcttcgta ataaaatgca tttaccaccg cgttatggtc
     3901 acagaaaaca aacccgtctc tttcttcccc gggccaatcc acaatatcct gtatgggttt
     3961 attgaaattt atagattgaa gaaaagcaga tgtttgcagg cctgtagtag tggaaatcaa
     4021 ccccttactg gttttaagtt gcatcaacga tctgtttttc agcaacggat aattcttttc
     4081 cagggatcga gcatcacccg tatacatata gtcataccaa gcgataagta tagcctgcaa
     4141 aatccattcg gtaggccagg tgggtttttt caagatatac tccagggatt tacgtgcaat
     4201 ggaatattcc ctatccacac tgtaatgact tagctgattg ataagtatgt ctgcttcata
     4261 cgggatacgt tctctgtctc catcaacgta aactcctgtg aatgaagttg ctttcatcgt
     4321 atacttactc atctcccaga tttgattcag ggtatcatta tcgcagcgga agaaggaggt
     4381 tgaatcatcg aatggataat gaacagattc ccttatgaca tcctctttat ccaacttatt
     4441 gccgtaatgc agcacttccg cataacggaa aggaactacc tcgcctatgt aagcaggcat
     4501 ttttacggca acttccaatg tgtttcgttt atctttatgc acttttatac ggtacaggtg
     4561 agtacctttc aataacgcca aagagtagga ttgataacga atggttcctc ctggccggga
     4621 gtccacacgc ccatcccgga ctttctctcc caggtttacg ataaccgtat cattcccggt
     4681 ttcagacgtc agtctgacaa gcaattgccc gaaggatgcc ttaccaaagt caaaaaacca
     4741 attgtcatcg cgggtcgaac acagaaccgg atgttcgaca gacttaacaa gaacctcact
     4801 cgaagattgg tatggcttta actcatctcc tgttctaaaa gatttgatat ctgaccaatc
     4861 gctttcttcg ctattgtcgg ttatcgcctt gactctccag tagtacactt ttcgtggctg
     4921 caacgacctg ccccggtacg caacggagac agacctgccg gagtttaccg tcccgctgtc
     4981 ccacacattc ccttcccctt tttgaataag gttcaaagag tccgacaaaa taatcctgta
     5041 accggattgt agcgtgttct tccctttgtt gggaacaatc caactgaacg atgggtactc
     5101 cgagaggatt ttggctgtct gaacgggttc aatagctgaa tcgatctctt catgatccag
     5161 ggtagaagga taaccgttga taaaaacctc ccctgttttt ttcaacaaat ccgtcatcaa
     5221 acccgtgggt ttgttttgcg catatgtgca gaaaaccacc agcgcaaaat acacaagcaa
     5281 gcatccttgt tttctcattt taaatcctgt tctttcttct tagagtttgg gtaacttcca
     5341 gggttc
//
LOCUS       DAOSDR010000224         5334 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrimonas sp. isolate AMR_MDS_1826
            NODE_5484_length_5334_cov_3.766622, whole genome shotgun sequence.
ACCESSION   DAOSDR010000224 DAOSDR010000000
VERSION     DAOSDR010000224.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863355
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrimonas sp. (wastewater metagenome)
  ORGANISM  Petrimonas sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Dysgonomonadaceae; Petrimonas.
REFERENCE   1  (bases 1 to 5334)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5334)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:10:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,720
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 35
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5334
                     /organism="Petrimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5484_length_5334_cov_3.766622"
                     /isolate="AMR_MDS_1826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2023866"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2340)
                     /gene="gyrA"
                     /locus_tag="PLI30_09205"
     CDS             complement(<1..2340)
                     /gene="gyrA"
                     /locus_tag="PLI30_09205"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005777867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HOI79768.1"
                     /translation="MIDQEDRVIKINIEDEMKSAYIDYSMSVIVSRALPDVRDGFKPV
                     HRRILFGMSELGNTSDKPHKKSARIVGEVLGKYHPHGDSSVYNAMVRLAQEWSMRYTL
                     VDGQGNMGSVDGDSPAAMRYTEARMNKLGEEMLRDIDKETVDFQFNFDDTLQEPTVLP
                     TRIPNLLINGASGIAVGMATNMAPHNLTEVINGIIAYIDDKEIDTEGLMHYIKAPDFP
                     TGGYIYGYKGVIDAFETGRGRIVMRGKAEIESGKNHDKIIISEIPYLVNKAELIKHIA
                     DLVGEKRIEGISNVNDESDRQGMRIVVDVKRDANANVVLNKLYKLTAMQSSFSVNNIA
                     LVNGRPEMLNLKRMIELFVEHRHDVVVRRTKYELRKAEERAHILQGLIIASDNIDEVI
                     AIIRGSSTPQEAIQRLIERFELSDIQARAIVEMRLRQLTGLEQDKLHAEFEEIQRLIA
                     HLNDVLNNEELRMQIIKEELIEVRDKFGDQRRSEIIFASEEMNPEDFYSDDEMIITIS
                     HFGYIKRTPLTEFRTQNRGGVGAKGSQTRDEDFVEYIYPATNHNYMLFFTQKGKCYWL
                     KVYEIPEGTKTSKGRAIQNLLNIDSDDSITAFVRVLTLDDEEYINSNYLVFGTQSGVV
                     KKTLLEAYSRPRQNGVIAIGLREDDQVISVRLTDGKKDIIMANRNGRAIRFNEEDVRP
                     MGRTATGVKGMTLDEDGEDYVIGMICMDPDSKDTILVVSEQGYGKRTMLNDEDGEPVY
                     RITYRGGKGVKTLNITEKTGKLVAIKSVTDDNDLMIINKS"
     gene            complement(2614..>5334)
                     /gene="secA"
                     /locus_tag="PLI30_09210"
     CDS             complement(2614..>5334)
                     /gene="secA"
                     /locus_tag="PLI30_09210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009319052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HOI79769.1"
                     /translation="GEGKTLVATLPVFLNALTHRGVHVVTVNDYLSKRDSEWMGPIYM
                     FHGLSVDCIDKHEPNSDARRKAYEADITFGTNNEFGFDYLRDNMAVSPKDLVQRIHNY
                     AIVDEVDSVLIDDARTPLIISGPVPKGDDQLFDLFRPRVEQVVKVQREFTTRLLADAK
                     AKMASADKKEQEEGALLLFRSYKGMPKYKPLIKFLSEQGVKAAMLKTEEFYMQEQSRN
                     MHIATDPLYFVIDEKQNSIELTDKGIDLLTGKSDDPYFFVLPDIASQLAELDNLPLSD
                     EEKAAKKDELLQNYAIKSERVHTVNQLLKAYTLFEKDDEYVVIDNKVKIVDEQTGRIM
                     EGRRYSDGLHQAIEAKERVKVEAATQTFATITLQNYFRMYNKLAGMTGTAETEAGELW
                     DIYKLDVVVIPTNRPIVRNDMNDRIYKTKREKYTAVIDEIENTISKGRPVLVGTTSVE
                     ISELLSRMLTLRKIKHNVLNAKLHQREAEIVANAGQRGMVTIATNMAGRGTDIKLTPE
                     VKDAGGLAIIGTERHESRRVDRQLRGRAGRQGDPGSSVFFVSLEDDLMRLFATERIAG
                     LMDRMGFKEGDMLEHDMLNKSVERAQKKVEENNFGIRKRLLEYDDVMNSQREVIYKRR
                     RHALMGDRIEIDVTNMITETAKNVVEANEDDFENMKLDLFRVMAVEIPFSEKEMKEMK
                     IDVQVEKVVQKAISGFKRKTDRLAEIAQPVIKDVYEKQGAMYENILIPISDGKRIYNI
                     ATNLKEAYESGSKALVKSFEKSILLHTIDEDWKEHLREMDELRHSVQNASYEQKDPLL
                     IYKLESFSLFKIMVNDINSKAVSILMRGQIPVQDPKNVRQAAPEQKTDYSKYRTQKDE
                     IEGGPSSNGSPRQPEQRKLEPVRVEKKVGRNDPCPCGSGKKFKNCHGQGA"
BASE COUNT         1264 a         1341 c         1084 g         1645 t
ORIGIN      
        1 cgatttgtta ataatcatta aatcattatc gtctgtcaca ctttttattg cgacaagttt
       61 tccggtcttt tctgtaatgt tcagcgtttt aacccctttc ccgcctcggt aggtaatcct
      121 ataaaccggt tccccatcct cgtcattaag cattgttcgc ttaccatacc cttgttcgga
      181 tacaaccagg atagtgtctt ttgagtcggg atccatgcaa atcataccga ttacataatc
      241 ttcgccgtcc tcatccaacg tcattccttt tactccggtt gcagtacggc ccatcggtct
      301 cacatcctct tcgttgaaac gaatagcccg gccatttcgg ttagccataa tgatgtcctt
      361 tttaccgtca gtcaacctta cggaaatcac ctgatcatcc tccctcaatc ctatggcaat
      421 caccccattt tgacgcggtc gcgaataagc ctccagcaat gttttcttaa caacccccga
      481 ttgtgttccg aaaaccaggt aattggagtt gatgtactct tcgtcgtcca gggtaagcac
      541 tcgtacaaaa gcggtgatgc tgtcatctga atcaatgttc agcaggtttt gaatggctct
      601 tcctttcgat gtcttggttc cttccggaat ttcgtaaacc ttcaaccagt aacatttgcc
      661 tttttgagta aagaagagca tataattgtg gttggtagcg ggataaatgt attccacaaa
      721 atcttcgtcc cttgtttgcg accctttagc tcctactcca cccctgttct gcgtgcggaa
      781 ctccgtcagt ggggtacgtt tgatgtaacc aaaatgagag atggtaataa tcatttcatc
      841 atcggagtaa aaatcttcgg gattcatctc ttccgaagca aaaatgattt cagaacggcg
      901 ttgatcaccg aacttgtcgc gcacttcaat caactcctct ttgataatct gcatacgcag
      961 ttcctcatta ttcaggacat cgtttaaatg agcgatcaac cgttgaattt cttcgaattc
     1021 ggcatgaagc ttatcttgct ccagcccggt aagttgcctt aaccgcatct ctactatggc
     1081 acgtgcctgg atatcggaca actcaaagcg ttcgatcaac cgttgaatgg cctcttgcgg
     1141 tgtgcttgaa ccgcggatta tggcaattac ctcgtcaata ttatcggagg caataatcag
     1201 accttgcaat atgtgtgccc tttcttctgc tttgcgaagt tcatattttg tacgtcgcac
     1261 aaccacatcg tggcggtgtt caacgaacag ttcaatcatc cgcttcaggt taagcatttc
     1321 cggacggcca ttcaccaacg caatattgtt cacactgaaa gacgactgca tggcagttaa
     1381 tttgtatagc ttgttaagaa caacgtttgc attagcgtcg cgtttgacat caacaactat
     1441 acgcattccc tggcggtccg actcatcatt tacgtttgat attccctcaa tcctcttttc
     1501 gcccaccaag tcagcaatgt gcttaatcaa ctccgctttg tttaccagat acgggatctc
     1561 tgaaatgatt attttatcgt ggttttttcc cgattcaatt tcggcttttc cacgcataac
     1621 gatgcgtccc ctacccgttt caaaagcatc aataacccct ttataaccat aaatataacc
     1681 acctgtggga aagtcgggag ccttgatgta atgcatcaac ccttcagtat ctatctcttt
     1741 atcatcaata tatgctataa ttccgttaat cacttccgtt aaattgtgcg gtgccatgtt
     1801 agttgccatt cctaccgcaa tacccgatgc gccgttgatg agcaggttgg gtatccgtgt
     1861 cggcaaaacc gttggctcct gaagagtatc gtcgaaattg aattgaaaat ctacagtttc
     1921 tttatcgata tcgcgcaaca tttcttcgcc aagtttgttc atcctggctt cggtataacg
     1981 catagccgca gggctgtcac cgtcaacact tcccatgttt ccctgaccgt caaccagcgt
     2041 gtagcgcatg ctccactctt gcgccaggcg caccatggca ttataaaccg atgaatcgcc
     2101 atgcggatgg tacttcccga gtacctcccc tacgattcta gccgatttct tgtgcggctt
     2161 atccgatgtg ttccctaatt cggacattcc aaaaaggata cgtctgtgca cgggtttaaa
     2221 tccatcacga acgtcaggta atgcacgtga aacgattacg gacatcgaat aatcgatgta
     2281 cgccgacttc atttcatctt caatgttgat cttaatgact ctgtcttctt ggtcaatcat
     2341 attaaaatgt aatttttttc ttcagacatg tcttatattt tgttgaaata cagaataata
     2401 aacaggcctt ttacttcttt ttaaataacg tactaaggta cggattttcc acggtttaac
     2461 aaaagaaatc caatcaaaaa tcgtttgcct cagaccgtaa ttaatgtttt ttattgtttg
     2521 ttccagaagg ttttttccaa caaaaaacaa cccgaagcaa gttaaatttg cttcgggttt
     2581 gtttgttctg ttgttttatc ttttagacta atgtcaggca ccttgtccgt gacaattttt
     2641 gaattttttc ccgcttccgc aagggcatgg atcgtttcga cccactttct tttccacgcg
     2701 gactggctct agttttcttt gttcaggctg tctaggtgaa ccattggatg agggcccccc
     2761 ttcaatttca tccttttgag tacggtattt gctgtaatcg gttttctgtt caggggcagc
     2821 ctggcgaacg tttttaggat cctgaacggg aatctggcca cgcatgagga tagaaaccgc
     2881 ctttgaattg atgtcgttga ccattatttt aaatagacta aacgattcaa gcttataaat
     2941 cagtagcggg tctttttgtt cgtaactggc gttttgaacc gaatgtcgta attcgtccat
     3001 ttcccggaga tgttccttcc agtcttcgtc gatggtgtgg agtaaaattg atttttcaaa
     3061 cgatttgacc agcgcttttg accccgattc atacgcttct ttcaggttgg tagcaatgtt
     3121 gtatattctt tttccatcgg aaatggggat aaggatgttt tcatacatgg caccctgttt
     3181 ttcgtacaca tccttgataa ccggctgggc aatttctgcc aatcgatccg ttttgcgctt
     3241 gaatcccgaa atagcctttt gaaccacttt ttcaacttgc acgtcgattt tcatctcctt
     3301 catctctttt tcggagaaag gaatctcaac tgccattaca cggaaaaggt ccagtttcat
     3361 gttttcgaaa tcgtcctcgt tagcctccac cacattcttg gccgtctccg ttatcatgtt
     3421 ggtaacatca atctcgatac ggtcgcccat caatgcatga cggcgacgtt tatagatcac
     3481 ttcgcgttga gaattcatca cgtcatcgta ttccaacaac cgtttacgaa taccaaagtt
     3541 gttttcctcc acttttttct gggcacgttc caccgatttg ttcaacatgt cgtgctcaag
     3601 catatcgcct tccttgaagc ccatccggtc cataagcccg gcaatccgtt cggtggcaaa
     3661 caagcgcatc aggtcatctt cgagagatac gaaaaaaacc gaagagcccg gatctccctg
     3721 acggccggca cggccacgta actgacggtc gacacggcga ctttcatgcc gttccgtacc
     3781 tataatagcc aaccctccgg catcttttac ctccggcgta agtttaatat cggtaccacg
     3841 ccccgccatg ttggtagcga tggttaccat tccacgctgt ccggcattgg caacaatttc
     3901 cgcttcgcgt tgatgtaatt tggcgttcaa cacgttgtgt tttatttttc gaagtgtaag
     3961 catcctgctt aacagttcgg aaatctctac tgaggttgtt cccaccagaa ccgggcggcc
     4021 tttagaaatg gtattttcta tttcgtcgat taccgctgta tatttctcac gtttggtttt
     4081 atagatgcgg tcgttcatat cgttacggac aatgggccgg ttggttggaa tcaccaccac
     4141 gtccagcttg taaatgtccc aaagttcacc agcctctgtt tctgctgtcc cggtcatacc
     4201 cgcaagcttg ttatacatcc tgaagtagtt ttgcaaggta atggtggcaa aggtttgcgt
     4261 cgcagcttcc actttcaccc gttcctttgc ctcaatagcc tgatgcaacc cgtcggaata
     4321 acggcgtcct tccatgatac ggcctgtttg ttcatccacg attttcactt tgttgtcgat
     4381 caccacgtac tcgtcgtctt tctcgaacag ggtgtaagct ttaagaagct ggtttaccgt
     4441 atgaacacgt tccgatttta tcgcataatt ctgtaataac tcatcctttt tagccgcttt
     4501 ttcttcgtcg gaaagaggta ggttatcgag ttcagccagt tgcgatgcaa tatcaggcaa
     4561 cacgaaaaag tacgggtcgt ccgacttccc ggtcagcaag tcgatccctt tatcagtcaa
     4621 ctcgatgctg ttttgttttt cgtcaataac gaagtacaac gggtcagtgg cgatatgcat
     4681 gttgcgcgac tgctcctgca tataaaactc ctcggtcttc agcatggccg ctttcacgcc
     4741 ctgttcactc aaaaacttga tgagcggctt atatttcggc atacctttgt aggaacggaa
     4801 cagcaataga gcgccttctt cctgttcctt tttatctgcc gaagccattt tggcttttgc
     4861 atcagcgagc aaccgggtag tgaactcacg ctggactttt accacctgct ccacccgcgg
     4921 acggaaaaga tcaaacaact gatcatcgcc tttcggaacg ggtcccgata tgatcaaggg
     4981 ggtacgcgca tcgtcaatta atacggagtc aacctcgtcc acgatggcat agttgtgtat
     5041 acgttgcacc agatctttgg gtgaaacagc catattgtcg cgaaggtaat cgaacccaaa
     5101 ctcgttgttc gtaccaaagg taatgtcggc ttcgtaagcc ttgcggcggg cgtcggagtt
     5161 gggttcatgc ttatcgatac aatctaccga aagcccgtgg aacatgtata ttggccccat
     5221 ccactccgag tcacgtttgg acaagtaatc gtttacggta accacatgca ctcctctgtg
     5281 tgtaagagca ttgaggaata ccggcagggt ggccaccaac gttttccctt cacc
//
LOCUS       DAOSDR010000225         5330 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrimonas sp. isolate AMR_MDS_1826
            NODE_5489_length_5330_cov_3.349953, whole genome shotgun sequence.
ACCESSION   DAOSDR010000225 DAOSDR010000000
VERSION     DAOSDR010000225.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863355
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrimonas sp. (wastewater metagenome)
  ORGANISM  Petrimonas sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Dysgonomonadaceae; Petrimonas.
REFERENCE   1  (bases 1 to 5330)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5330)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:10:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,720
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 35
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5330
                     /organism="Petrimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5489_length_5330_cov_3.349953"
                     /isolate="AMR_MDS_1826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2023866"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            182..1207
                     /locus_tag="PLI30_09215"
     CDS             182..1207
                     /locus_tag="PLI30_09215"
                     /inference="COORDINATES: protein motif:HMM:NF025465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PmoA family protein"
                     /protein_id="HOI79770.1"
                     /translation="MKINFFILMVLLFAVIFSCTSQPLQKVEFKNLPHDKKVEVHIDG
                     KFFTAYIYPDNMEKQSLYPIVSASGKTITRGYPMNPRPFERTDHPHHIGLWFNFGDVN
                     GLDFWNNSFAIKAADKPKYGTVKFKKIVSENPKKGELVTASEWVDVNNKVLLNEESTF
                     IFSGTDNMRTIERITKLTAVQPVTFTENKEGLIGLRLDRAFEEPATKPEKFLDANGIE
                     TEVPVLNNEGVNGVYRNAEGVSGGAVWGKRSPWVALRADKQGEIITIVILDHPKNPNY
                     PAWSHARGYGLFATNNLGGRAFDKNTSPVKLELQPGEKIVFKHKVVIGGDLTDEQIAE
                     LAVNFKN"
     gene            1233..2105
                     /locus_tag="PLI30_09220"
     CDS             1233..2105
                     /locus_tag="PLI30_09220"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HOI79771.1"
                     /translation="MQKKCVFCRIIVLMMVLTIFSCVSPKQKSFLTEIGVCASVSTAE
                     MLAAHGYTYIEESVGRFLMPDKSEEEFNEKLQQARNAVIPVKACNSFIPGSLKSVGPQ
                     AVHPEILEYMETAFRRAQKAGVEYIVFGSGGSRSIPEGFPREEAHRQFIALCSQMAPI
                     AAQYNVVVVLEPLNTKECNFINSVSEGGEIVEEVNHPNFRLLADIYHMLMDGESPESI
                     EKYGHLIQHTHIAEKEGRAAPGIHNEDFTAYFKALKNVRYNGKMSIECKWENLEVQAP
                     AAIETMKNQLSILK"
     gene            2156..3472
                     /locus_tag="PLI30_09225"
     CDS             2156..3472
                     /locus_tag="PLI30_09225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008154480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOI79772.1"
                     /translation="MKGTAAAAIAPLVLKTEKNAPIILSSSAKGSNDRIRIAVLGING
                     RGKSHIEEIMKLSEKANVEVAVLCDPDMNLLRERAKEFEDKYGKKVAIEQDFRKTYDD
                     RNIDAVALATPNHWHALQTIWACQAGKDVYVEKPATHNIYEGKKMIEAAYKYNRIVQH
                     GVQLRSSPAIREAVKHLEDGLIGRVYMSRGLVYRWRPDIGNKGIAKVPTGLNYDLWCG
                     PAPMRPFSRNLVHYNWHWHWDYGNGDVGNQGIHETDLCMWGLGVNSLPEKITSMGGKF
                     LWNDCKEVPEIQTSIYHYPKEKKIIQFEVRNWCTNLEDGAGVGNIFYGEKGYLVVKGY
                     DTYETYLGQNREKGPSRSEKGELTLHFQNWFDAIRARDMSIQNGPVQSGHLSSSLAHL
                     GNISYRLGRQLEFDPVAERFIGDGENEANSMLSREYRSPYLLPQNV"
     gene            3901..>5330
                     /locus_tag="PLI30_09230"
     CDS             3901..>5330
                     /locus_tag="PLI30_09230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019540079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOI79773.1"
                     /translation="MRFLLTNILIVFCAFTSFAQKNKQYDVVIYGGTSAGIASAIQTS
                     RMGKSVVLVEPTNRLGGLTTGGLGQTDIGNKHAIGGISREFYQKIRTYYDDPQNWQWQ
                     KRAEYMSDGQSRTEKGEDAMWTFEPSAALKVYEQMLNREKVEIVYGERLNRKTGVLKK
                     EGKVVSITMENGSKYTGKMFIDATYEGDLMAASGVSYSVGRESNSEYGETLNGVQANR
                     INRTLKWTLSRNAYNHNFIDGVDPYIIKGDPSSGLLPYIVEGGRPGIDGRGDKGIQAY
                     CFRMTLTNHPENRIPFKKPDNYNELNYELLFRNYEAAVGPIEEMYPYGDKLVPWINSP
                     MPNKKTDTNNQKGFSTDFIGQNYDYPEASYAEREKIVQAHRDYQQGLMWTLAYHPRIP
                     QKVREVVSTWGTCKDEYEPGSDGWQQQLYIREARRMKSDYVMSQKNCERIEVIDDPVG
                     MGAYGMDSHNVQRYVDANGFVQNEGNV"
BASE COUNT         1746 a         1034 c         1183 g         1367 t
ORIGIN      
        1 gctacagaga ctatgtaaaa aaggaacatt atctgaaacc gctgccactt gaacaagtaa
       61 atcgttttaa aaacatcact acggaaatgc ttactccagg atatgactat ggcgctctaa
      121 actaatctgg cattataaca tattcttgaa acaaaaatca tatatactaa aaccaccgac
      181 tatgaaaatt aatttcttca tattaatggt attactattt gcagtgattt tttcgtgtac
      241 ttctcaacct cttcaaaagg ttgaattcaa aaatctcccg catgataaaa aagttgaagt
      301 gcacattgac ggaaaatttt ttacggcata catttatcct gataacatgg aaaaacaatc
      361 actttacccg attgtcagtg catcaggaaa aaccataacc cgtggttatc cgatgaatcc
      421 acgcccattt gagcgaaccg atcatccaca tcatatcgga ttgtggttca actttggtga
      481 tgttaacgga ctcgacttct ggaataattc atttgccatc aaggcagccg acaagcccaa
      541 atacggaact gttaaattca aaaagattgt ttctgaaaat cctaaaaaag gagaattggt
      601 gacagcatcg gaatgggtag atgtcaataa taaagtttta ctcaatgaag aatctacctt
      661 tattttttca ggaacagata atatgaggac gattgaacgg ataaccaagc ttactgcagt
      721 gcaacctgtt acctttactg aaaacaaaga aggtttgatt ggattgcgcc tcgatcgtgc
      781 ctttgaagaa cctgccacca aacccgaaaa atttctggat gccaatggaa ttgaaacgga
      841 agtaccagta ttaaacaacg aaggtgttaa cggagtttac cgtaatgctg agggtgttag
      901 cggtggtgca gtttggggta aacgctctcc ctgggtagct ttgcgggccg acaaacaagg
      961 tgaaatcatc accatcgtta ttctcgacca tccaaagaat ccgaattatc cggcctggtc
     1021 gcatgcgcgt gggtatggct tatttgccac caacaatctt ggcggtcgtg ccttcgataa
     1081 aaatacttcg ccggtgaaac tggaattgca acctggagag aaaattgtat tcaaacacaa
     1141 agtggtgatt ggtggcgatc ttactgatga gcaaattgca gagttagctg taaattttaa
     1201 aaactaaaat cattgaaaaa tcaaaaaacg atatgcaaaa gaagtgtgtt ttctgcagaa
     1261 ttattgtatt gatgatggtc ctgaccatct tttcctgcgt ttcaccaaag caaaaatctt
     1321 ttttaactga aatcggtgta tgcgccagcg tttccactgc cgagatgctg gcggctcacg
     1381 gatatactta tattgaggaa agtgtggggc gatttttaat gcccgacaaa agcgaagagg
     1441 aatttaacga aaagctgcaa caggcccgga atgctgttat cccggtaaaa gcctgcaaca
     1501 gttttattcc cggaagttta aaaagcgttg ggccgcaagc agttcatccc gaaatcctgg
     1561 aatatatgga aacagcgttc cgcagggcgc aaaaagccgg agtggaatac atcgtttttg
     1621 gtagtggcgg ctcacgttct atccccgaag gatttccgcg cgaagaagca catcgccagt
     1681 tcattgccct ttgcagccag atggcaccca tcgcggcaca atataatgta gtggtggttc
     1741 tggaacctct caataccaaa gagtgtaact ttatcaattc ggtttccgaa ggcggggaga
     1801 ttgtggaaga agtgaatcat cccaacttca ggctgctggc tgatatttac catatgctga
     1861 tggatggcga aagccctgaa agtatcgaaa aatatggtca ccttattcag catactcaca
     1921 ttgcagaaaa agaaggacgt gcagcgcctg ggattcataa cgaagatttt actgcctatt
     1981 tcaaagcctt aaaaaatgtt cgttacaatg gaaaaatgtc aatcgaatgc aagtgggaaa
     2041 atttggaagt gcaggcaccc gctgcaattg aaacaatgaa aaatcagttg tcaattttaa
     2101 aataagaaaa atggaaaata ccaataagtc aagaagacat ttcttaacaa ccacaatgaa
     2161 agggaccgca gcagctgcta tagcaccttt ggttctaaaa accgaaaaaa acgctcctat
     2221 catactctcc tcttctgcaa aggggtccaa tgaccggatt aggattgcgg tgctagggat
     2281 taacggacgt gggaaaagcc atattgaaga aatcatgaag ctttccgaga aagcgaatgt
     2341 tgaagtggca gttttgtgtg atccagacat gaatcttttg agggaacgcg ccaaagaatt
     2401 tgaagataaa tacggaaaaa aagttgctat tgaacaagac tttagaaaaa cttatgatga
     2461 tagaaatatc gatgcagtag cccttgcaac gcctaatcac tggcatgctt tgcagacgat
     2521 ctgggcatgc caagcgggga aagacgttta cgttgaaaag cctgctacac acaatatcta
     2581 cgaaggaaaa aaaatgattg aggcagctta taaatacaac cgaattgttc aacacggagt
     2641 acaactccgc agctcaccag ctataagaga agcggttaaa catcttgagg atggtttaat
     2701 tggcagagta tacatgtccc gaggattggt ttatcggtgg cggccagata taggaaacaa
     2761 agggattgct aaagttccga caggattaaa ttatgattta tggtgtggac cagctcctat
     2821 gcgtccattc tctcgcaatc ttgttcacta caactggcat tggcattggg actatggcaa
     2881 cggagatgta ggtaatcagg gcattcacga aacagatctt tgcatgtggg gattgggggt
     2941 taatagtctt cctgaaaaaa tcacttcaat gggagggaaa tttctctgga atgactgcaa
     3001 agaagttcct gaaatacaaa catctatcta tcactaccca aaagaaaaaa agattattca
     3061 gttcgaagtt cgtaactggt gtaccaactt ggaagatggc gcaggtgttg gcaatatttt
     3121 ttatggagaa aaaggatatc tagtagttaa aggttatgat acctacgaaa cctatttggg
     3181 acagaatcgg gaaaaagggc ccagtagatc tgaaaaaggt gaattaacac ttcatttcca
     3241 aaactggttc gatgctatac gggcacgcga catgagtatt cagaatggtc ccgtacaaag
     3301 cggacattta tcctcttcgc tggctcattt gggtaatatc tcttacaggc tcggacgtca
     3361 gcttgaattt gatcctgttg cagaacgttt tattggtgat ggagaaaatg aagccaattc
     3421 aatgctcagc cgagaatatc gatcgcctta tcttttgcct caaaatgtat aatttattta
     3481 ctttcttaaa aaatgacagc agaaattccc ttagccagag agcttctgtt gttgagcgaa
     3541 ctacaacgaa atcaacctaa tacctttcag ctacttttaa agaagagaga aggcagtcgt
     3601 attggactgc cttctctcat ccctgtggag gaccgaaaca gacaaccatc tcttcggttg
     3661 ttaggaaaca ttttgttcaa attgagctgt ttctacagcc ggctgaccta cccttcctca
     3721 tccgctgtta agtatgttaa tgataagcat ttcggagacc gtaaatagaa cgatgaaggc
     3781 ggcttttgct ataattattc tgaattaaag cattttttaa agagccagtc tctcatcaat
     3841 caaaactatt tgatacttta cggataatct tatctaaaca atcataaaaa aactacaaac
     3901 atgaggttct tattaacaaa tattttgatc gttttttgcg cattcacatc ttttgctcaa
     3961 aaaaacaagc agtacgatgt ggtaatatac ggaggcacat ccgctggtat tgcatctgcc
     4021 atacaaacct cgagaatggg taaatcggta gtccttgttg agccgacgaa cagattaggc
     4081 ggtctgacaa ccggaggatt gggacaaacc gatatcggga acaaacatgc cataggagga
     4141 atttctcgag aattttatca gaaaatcaga acgtattacg acgatcccca aaattggcaa
     4201 tggcaaaaac gcgcagaata tatgagcgac ggacaatcac gcacagaaaa aggtgaagat
     4261 gccatgtgga cctttgagcc ttcggcggct ttaaaagtgt atgagcaaat gctgaatcgt
     4321 gaaaaagttg aaatcgttta tggagaaaga ttgaatcgga aaaccggagt tctaaagaaa
     4381 gaaggtaaag tggtttcgat aacaatggaa aatggctcaa aatataccgg aaaaatgttt
     4441 atcgacgcga cctacgaggg agatctgatg gcagcttcag gagtatccta cagcgttgga
     4501 agagagtcaa acagtgagta tggagaaacc ttgaacggag ttcaggcaaa ccgaatcaac
     4561 agaacattaa aatggaccct ttccagaaac gcatataacc ataattttat tgacggggta
     4621 gatccgtata ttattaaagg tgatccaagc agtgggttgt tgccctatat agtggaagga
     4681 ggacgtccgg gaattgacgg tcgcggtgac aaaggcatac aagcttattg tttccgaatg
     4741 acgcttacca atcatcccga aaacaggatc ccttttaaga aacccgacaa ctacaacgag
     4801 ctgaactatg aacttctatt cagaaattat gaagccgctg tcggtcccat tgaagagatg
     4861 tacccctatg gcgataaatt agtcccctgg ataaactccc ccatgccgaa taaaaaaacc
     4921 gataccaata atcaaaaagg gttttctacc gattttatag gacagaacta cgattacccg
     4981 gaagcaagtt atgcggagcg ggaaaaaatt gttcaagccc acagagatta tcagcaagga
     5041 ttaatgtgga cactggctta tcacccccga attcctcaaa aagtgagaga ggtagtttca
     5101 acgtggggca cttgcaaaga tgaatatgaa ccgggaagcg atggatggca acaacaactt
     5161 tacatcagag aagcacgtcg gatgaaaagc gactatgtaa tgtcacagaa aaactgcgaa
     5221 cgtattgaag ttattgatga cccggtggga atgggagctt atggaatgga ttcacataat
     5281 gtgcaacgat atgtggatgc caacggcttt gttcaaaacg aaggcaatgt
//
LOCUS       DAOSDR010000226         5327 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrimonas sp. isolate AMR_MDS_1826
            NODE_5495_length_5327_cov_4.120068, whole genome shotgun sequence.
ACCESSION   DAOSDR010000226 DAOSDR010000000
VERSION     DAOSDR010000226.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863355
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrimonas sp. (wastewater metagenome)
  ORGANISM  Petrimonas sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Dysgonomonadaceae; Petrimonas.
REFERENCE   1  (bases 1 to 5327)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5327)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:10:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,720
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 35
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5327
                     /organism="Petrimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5495_length_5327_cov_4.120068"
                     /isolate="AMR_MDS_1826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2023866"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(125..1378)
                     /locus_tag="PLI30_09235"
     CDS             complement(125..1378)
                     /locus_tag="PLI30_09235"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF015692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HOI79774.1"
                     /translation="MRKTILKIAVIIFTMSLTGCTSLTRKNDLDIKYRRLPVREYRDK
                     LQAGWLGQIVGVSWAAPTEFKWKDEIIPLEEMPLWGHEMINEAFNQDDLYVEMTFLRS
                     LEEYGISVSIRQAGIDFANTEYPLWCANETGRRNLRMGIAPPDCSHPAFSNNPHDIDY
                     QIEADYSGLIAPGMPNVPIALGDTFGRLMNYSDGVYAGQFVGGMYSEAFFEDDIIKII
                     EAGLACIPEGCQYAEMVRDVVSWYKANPTDWKATWELCQEKYRRNPEYQKNSNGGIDV
                     KINGAYILIGMLYGEKDIDQTIIISTRCGQDSDCNPSNAAGVLFTTMGTSNLPDRFKE
                     NLDQETKFSHTPYNFPKLLDVCEKLAREYVVQEGGRVETDSNGEEVFVIPVKTPTPSP
                     LVFSWDPEPIAKSLFTEKEMEKIKH"
     gene            complement(1459..2388)
                     /gene="rbsK"
                     /locus_tag="PLI30_09240"
     CDS             complement(1459..2388)
                     /gene="rbsK"
                     /locus_tag="PLI30_09240"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR02152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004747 - ribokinase activity [Evidence
                     IEA];
                     GO_process: GO:0019303 - D-ribose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HOI79775.1"
                     /translation="MQKNQIVVIGSSNLDYIVTTDHFPKGGETLEGLSFTQAMGGKGA
                     NQAVAACRSGGAVSFITCVGNDLNGKNALAYYNKEGIDVSLALVKENVTTGTASIWVD
                     KHGENSIIVIPGANQYLSSDYIIPHSNRLIQANTILLQMEIPYETVKTICKISTGHTK
                     VILNAAPAYSIEDEILQSIYMLVVNETEAETISKCQIEQIGEEEMVKTLLQKGVKNVI
                     LTLGSSGCIYSNGTDWLKIPAFKVQVKDTTAAGDTFCGALAVGIGKYSEIKQALIYAT
                     AAAALCVTSLGAQPSIPDEQDIVTFLNQNNIYL"
     gene            complement(2487..3566)
                     /locus_tag="PLI30_09245"
     CDS             complement(2487..3566)
                     /locus_tag="PLI30_09245"
                     /inference="COORDINATES: protein motif:HMM:NF014826.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HOI79776.1"
                     /translation="MKTKALPYIYICLFLSFFFNSTAQVQNDEVYEYPNLNSQDYRYN
                     IRIPDIDGYKTLKCDFHVHTVFSDGKVWPDQRVKEAWNEGLDVIAITDHIEYRANKKI
                     LISDHNKSYEIAKAQGDAIGMLVIKGAEITRQKPLGHLNALFIQDANKLERPDAIEAI
                     DEAMKQGGFILWNHPGWPDDKSTMYPIHRELIEKQKIHGIEVFNGWEAYPKVIDWCQE
                     FNLAPISNSDIHYTSANLYRGKLMRPMTLVFAKDYTEQGVKDAMFSGRTLAFYNNVLS
                     GDEGLLKSLIKASLSVRVINEKNGMIEITNLSDIAYEIKYGEYMYPMPLYPNQVLRAT
                     IPTGTDVEFSNCICGKDRHITLKLW"
     gene            complement(3651..5198)
                     /locus_tag="PLI30_09250"
     CDS             complement(3651..5198)
                     /locus_tag="PLI30_09250"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019540315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HOI79777.1"
                     /translation="MNQLKLFFFYGAIALLSCNVGDTFAKDYKISKGELLNKIKGGWA
                     GQVIGCTYGGPTEFKWNGTMIGEEIGIPWDGSRMSWYYKNSPGLYDDVYMDLTFVQVF
                     DKYGLDAPDSLHAKYFANAGYPLWHANQAARYNILNGIMPPESGHWKNNPHADDIDFQ
                     IEADFAGLMSPAMVNAASEIGWRIGHIMNYGDGVYGGIYVAAMYALAFVHNDIEFIVE
                     EALKSIPKQSNYYQCIADMIQCYREDPNDWKKAWFEAQKKWTSDIGCPDGVFMPFNID
                     ATINGAYIVIGLLYGKGDYGATIDISTRCGYDSDCNPANAAGILGTMIGYDKIPAYWK
                     QGLDKVEDLNFAHTEMSLNKVYETGLRHAGEMIVRNGGRLDGDMFTIKYQQPEPVPFE
                     KSFEGLYPVERRRIGSSLTRKNREVTFKINGSGFVLGGRAMKNNNLPDVVLEIEVYIN
                     GNLYEVAKIPTDNRVRRHELTWNYDLKEGENNITLKAKEIPDGYRIETQDVIEYSKNK
                     PGKLIYY"
BASE COUNT         1365 a         1216 c         1065 g         1681 t
ORIGIN      
        1 gtaagaagca cgttgttttt tcctttgtag gtctcgcgga gtcccggctg catatcggag
       61 ccgcaaaaag cgattatcca tcccgggaat cgttggtcca gatcaccctg aatattcacc
      121 atggctaatg tttaatcttc tccatttctt tttccgtgaa aagagatttg gctatcggtt
      181 ccggatccca actaaacacc aatggacttg gagtgggtgt tttaacggga atcacaaata
      241 cttcttctcc gtttgaatcg gtctctacac gtccaccttc ttgcacaacg tattctctgg
      301 caagcttttc gcaaacgtca agcaatttcg ggaaattgta aggcgtgtgg gagaatttgg
      361 tttcctggtc aagattttcc ttgaatcggt ctggcaggtt cgacgtaccc atggtggtaa
      421 acaacacacc tgctgcattt gaagggttgc aatcggagtc ttgtccacaa cgggtagaaa
      481 taataattgt ctggtctata tccttctctc cgtaaagcat accgataagg atatatgctc
      541 catttatttt cacatctata ccaccgtttg agttcttttg gtattcggga tttctccgat
      601 acttttcctg acataactcc caagtggctt tccaatcggt cgggttggcc ttgtaccatg
      661 aaaccacgtc gcgtaccatc tcggcatatt ggcatccctc ggggatacat gctaaccctg
      721 cctcgatgat ttttatgata tcatcttcaa aaaacgcttc actatacata ccgccgacaa
      781 attgccccgc atacacaccg tcgctgtaat tcatcagtcg tccgaatgta tcacccaagg
      841 ctatcggcac attgggcatt ccgggggcga taagaccgga gtaatcggct tctatctgat
      901 agtcaatatc atgagggttg ttgctgaaag ccggatgact gcaatcggga ggggcaatcc
      961 ccatgcgcag gttgcgtctg ccggtctcat tagcacacca taaagggtat tccgtatttg
     1021 cgaaatcaat acctgcctga cggatagaaa cacttatgcc atactcttcc agagagcgca
     1081 agaaggtcat ttccacatag aggtcgtctt gattaaaagc ttcattaatc atttcatggc
     1141 cccatagtgg catctcctct aaagggataa tttcgtcttt ccacttgaat tcggtggggg
     1201 cagcccaact aacccccact atctggccga gccagcctgc ctgtaattta tctcgatatt
     1261 cccggacggg tagcctccgg tattttatgt caagatcgtt ttttcgtgtt aaggaggtac
     1321 atcccgtcaa gctcattgtg aagataataa cagctatttt taaaattgtt ttccgcattc
     1381 ttaaaaggat cattatttat aatttataat aagaagtgtg tataatttac agtaacagca
     1441 aatatgcact ttgaggtttt acaggtagat attgttttga tttaaaaagg tgacaatgtc
     1501 ctgttcatcg ggtattgaag gctgtgctcc aagtgaggtg acacatagcg ctgccgctgc
     1561 agttgcataa ataagtgctt gtttaatttc gctgtatttc ccaattccga ctgcaagagc
     1621 cccacagaaa gtatctcccg ccgcggtggt atcttttacc tgtaccttga atgctggaat
     1681 ctttaaccaa tcagtgccgt tgctgtatat gcatccgcta ctgccaaggg tgaggatcac
     1741 gtttttcacc cctttctgta aaagagtttt taccatttcc tcttcaccaa tttgctcaat
     1801 ttggcatttg ctgatggttt ctgcttcagt ttcattcacc accagcatat aaatggactg
     1861 taaaatctca tcttcaatac tgtaagcggg agcggcattt aagattactt ttgtatgccc
     1921 cgttgatatt ttgcaaatcg tctttactgt ttcataagga atttccattt gaagcaaaat
     1981 agtattggcc tgaatgaggc ggttggagtg gggaatgata taatcggagg agaggtattg
     2041 gttggcaccc ggaatcacaa tgatgctgtt ctctccgtgt ttatctaccc agattgaggc
     2101 agtcccagtg gtgacattct cctttacaag agcaagcgat acatctatcc cttccttgtt
     2161 ataataagcc aaagcatttt tcccattgag atcgtttcct acgcaagtga tgaaacttac
     2221 ggctccgcct gatctgcagg ctgcgaccgc ctggttggcc cctttgcctc ccatggcttg
     2281 tgtaaatgat aatccttcaa gggtttcccc tcctttgggg aaatgatccg tagttacgat
     2341 atagtccaag tttgaacttc ctataacgac aatttgattc ttttgcatat tatatataag
     2401 atttgaccct gaaatgatat tcaggttggt aatgatcccg attatttttg ttagcttatg
     2461 tcgtacggtt taaaaaaagt gatttcttac cacaatttca acgtgatgtg acggtccttc
     2521 ccacatatac aatttgagaa ttcaacgtcg gtgccggtag ggatggttgc acgcaacacc
     2581 tgattcggat ataacggcat tgggtacata tactccccat acttgatttc ataagcgata
     2641 tcgctgagat tggtgatctc gatcatccca tttttctcgt taatcactct cacagaaagt
     2701 gaagctttga taagggattt cagtaatcct tcatctcctg acaaaacatt gttatagaat
     2761 gccagggttc tgcccgaaaa catcgcgtcc ttaactccct gttcggtata atctttagca
     2821 aataccaggg tcattggacg cattaacttc cccctgtaga ggttggcact ggtgtagtga
     2881 atatcagagt tggatatggg agccagatta aactcctgac accagtcaat aaccttcgga
     2941 taagcctccc aaccattaaa aacttctatt ccgtgaattt tctgcttttc aattaattct
     3001 ctatgaatag ggtacattgt gcttttatca tccggccaac cgggatgatt ccataaaata
     3061 aaaccacctt gtttcattgc ctcatcgatc gcttcaatcg catccggacg ctcaagcttg
     3121 ttcgcatctt gaataaaaag ggcgttcagg tgacccagcg gtttctgacg cgtaatttct
     3181 gctcctttta ttaccagcat cccaatggca tctccctggg cttttgcgat ttcataggat
     3241 ttgttgtgat cgctgatcaa tattttctta ttggcccggt attcaatatg gtccgttatg
     3301 gctattacgt ccagaccttc attccaggct tccttgaccc gttggtctgg ccaaactttt
     3361 ccatcggaaa aaacggtatg cacgtgaaaa tcacatttca gcgttttgta tccgtcaata
     3421 tcgggtattc tgatgttgta tcgataatcc tgactgttta aattcggata ttcgtagacc
     3481 tcatcgtttt ggacctgggc agtactgttg aaaaaaaaac tgaggaacaa acagatgtaa
     3541 atgtaaggaa gtgcttttgt tttcatatat tcattgtatt tttcttacaa cgtgaattta
     3601 tttttttgat taaattgtag atgtgattct tttctatcct ttatgactga ttaataataa
     3661 attaatttcc ccggtttgtt tttggaatat tcaatcacat cctgtgtttc tattcggtac
     3721 ccgtcaggga tttctttggc ttttaatgtg atgttgtttt cgccctcctt caaatcataa
     3781 ttccaggtaa gctcatggcg tcttacgcga ttatctgtgg ggatttttgc cacttcatac
     3841 aagttgccgt tgatatatac ttcgatttcc aggacaacgt cgggcaggtt gttattcttc
     3901 atagccctgc ctcccaaaac aaaacctgaa ccattgatct tgaatgttac ttctctgttt
     3961 ttacgggtaa gactgcttcc aatccttctt ctctcaaccg gatacaaccc ttcaaagctc
     4021 ttttcaaaag gaacaggttc tggttgctgg tatttgatgg tgaacatatc tccatccagt
     4081 cgtccaccgt ttcggacgat catttctccc gcatgacgta aacctgtttc gtagaccttg
     4141 ttaagggaca tctcggtgtg tgcaaaattt aaatcctcta ctttgtccaa tccctgtttc
     4201 caatatgccg gtatcttgtc gtacccgatc attgttccta gaattccggc agcattggcc
     4261 ggattacagt ctgaatcgta cccgcatcgc gtactgatat caatagtcgc gccataatca
     4321 cccttgccat aaagtaagcc aataacaata tatgcaccgt tgattgtagc atcaatgttg
     4381 aaaggcataa aaactccgtc cggacaaccg atatctgatg tccatttttt ttgtgcctca
     4441 aaccacgcct tcttccagtc attcggatct tcacgataac actgtatcat atcggcaatg
     4501 cattgatagt agttgctctg cttagggata gatttcaaag cttcttccac aatgaattcg
     4561 atatcattat gcacaaaagc cagtgcatac atggccgcca cataaatccc gccgtatact
     4621 ccatcgccat aattcatgat atgcccaatt ctccagccaa tctctgatgc ggcattgacc
     4681 attgccggag acatcaaccc ggcaaaatcg gcttcaatct ggaaatcaat atcatcggca
     4741 tgtggattgt ttttccaatg tcctgattcg ggcggcatga taccgttcag gatattgtat
     4801 ctggcagcct ggtttgcatg ccataacggg tagcctgcat tggcaaagta ttttgcatgt
     4861 aatgagtcgg gtgcgtccag tccatacttg tcaaagacct gtacaaatgt taaatccata
     4921 taaacgtcgt catacaagcc cggggaattt ttgtaatacc acgacatgcg tgagccgtcc
     4981 cagggaatgc cgatctcttc tccgatcatt gtcccgttcc atttgaactc agtagggcca
     5041 ccgtaggtac atccaattac ctggcctgcc caacctcctt tgattttatt cagcagctct
     5101 cctttgctga ttttataatc ttttgcaaac gtgtcgccca cattgcacga taaaagggca
     5161 atcgcaccat aaaagaaaaa aagcttaagt tgattcatga gaattcagtt attgggtgta
     5221 taattttgag tcgaatccgt tatcagctgt gcaaatatag aataaaaaag atttatgaac
     5281 aaacgtttgt tcattttttg agaaaaaaac agccgatttc agtaatt
//
LOCUS       DAOSDR010000227         5311 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrimonas sp. isolate AMR_MDS_1826
            NODE_5516_length_5311_cov_3.523021, whole genome shotgun sequence.
ACCESSION   DAOSDR010000227 DAOSDR010000000
VERSION     DAOSDR010000227.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863355
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrimonas sp. (wastewater metagenome)
  ORGANISM  Petrimonas sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Dysgonomonadaceae; Petrimonas.
REFERENCE   1  (bases 1 to 5311)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5311)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:10:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,720
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 35
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5311
                     /organism="Petrimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5516_length_5311_cov_3.523021"
                     /isolate="AMR_MDS_1826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2023866"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..717)
                     /locus_tag="PLI30_09255"
     CDS             complement(<1..717)
                     /locus_tag="PLI30_09255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI79778.1"
                     /translation="MQKQIYFSAVFLFFLFFTGVLSAQDRMVMILGDTLDCKITGVTP
                     KYLYFERMQNGVVIKDRIDRADVREWSKTEQPEAFGQAARPVWGVGRFRIALSGGYGI
                     RTANTKTARQEMERVGFTSQEAKKYYKKLQAGEQAGGQLHYMFLPDLGLGVDYHFYYA
                     SSSIYGNFDPQDGVFRFYGEISENIYTNFVGISCYGQQWLKPERLKAYSVISVGMALY
                     RNETLFVYNPMLISGKALGEN"
     gene            complement(1019..2962)
                     /locus_tag="PLI30_09260"
     CDS             complement(1019..2962)
                     /locus_tag="PLI30_09260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019151926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HOI79779.1"
                     /translation="MTTNILSRTILSVLFVGLGLTSYTQKADKGEELHRLQYRNEGLT
                     VDLAVGLWSSPLPMDFDKDGDIDLVISCTDVPYNGTYFFENISGEQSANTVFRKPVRI
                     GQGITNTTISYVDGKARILGSGVEFSDFIKFAYDKPVGISNTSEILSLHPKKRFNHWK
                     YVDYENDGDLDLIVGIDDWADYGWDNAFNSAGKWTRGDLHGYIYLLENEGGRYVLRGK
                     MMAGDRPIDVYGNTSPNIEDFDGDGDLDIICGEFVDRFTWFENIGSRTKPAYAPGKFL
                     GNSKGLIQMDLEMMMPVALDWNGDKKIDLIVGDEDGRIAFIENTGAVNQGMPVFKSPV
                     YFKQKADLVKFGALVTPFSVDWDNDGDEDLICGNSAGYIGFIENLGGGTTPRWNKPEY
                     LKADGEVIRIMAGENGSIQGPCERKWGYTTLSVADWDGDGQKDILVNSIWGKVEWYRN
                     VGREGSPKLTKMGGVKVDWRSDVIPKPAWNWWNPKKDELVTQWRTTPYAIDWNKDGLM
                     DLVMLDHEGYLTFFERFRKEGKLLLQPGKCIFKDADLDHGNGLLRLNNREAGGSGRRK
                     FCFTDWDKDGDLDLLLDGKNIVLLENIGEKDGNTIYKSRGDLFDLRLAGHDTSPTIVD
                     WNKDGKPELLIGAEDGHFYYVSE"
     gene            complement(3001..4392)
                     /locus_tag="PLI30_09265"
     CDS             complement(3001..4392)
                     /locus_tag="PLI30_09265"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HOI79780.1"
                     /translation="MINNKFIKNYLRIGKYVGISLSILPTISGYANSRTVHERANNKP
                     NVILILADDMGIGDISKFNHGLTKTPNLDKLVVESLYFNNAYSAAPVCAPARASLLTG
                     QYPHRTGCVTLNMERFPELSRIKSGIPTMADYFRNEGYVTGLIGKWHCGMGEDYHPLK
                     RGFDEFEGFIDPFLIKSYFEYTLNINGKNHQFNDKYLTDDLTDRALGFIKRNKDKPFF
                     LHLAHYAPHRPLSAPDLLIDYYQKKGYNKNTATIYAMIEIMDNGIGKLLDELEKMKLR
                     KNTIIVFVSDNGPDPITGERFNQDLKGTKYTVLEGGIRVPFIFNWSGQIKPGLSNELI
                     HFTDVLPTLADICGLTIPESATLDGGSFYGILFNEDYQLPEIRIWQWNRGVPYYTHNA
                     AIRKGDWKLVRPFINTDIPEEESSLKPQLFNIADDPQEKNNLIDKNREEYLTLKVLLE
                     QKSRELEYDRLKK"
     gene            complement(4500..>5311)
                     /locus_tag="PLI30_09270"
     CDS             complement(4500..>5311)
                     /locus_tag="PLI30_09270"
                     /inference="COORDINATES: protein motif:HMM:NF019592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HOI79781.1"
                     /translation="IIHSGKFSLLQNFQNVFGGLYDNVHDNNEEILFAIQFLSTAECG
                     QSGEGSDLVLRYGIRGANITPYSILQARANDVFMNDFIIYNGIDNPRYKTTFLTEYTD
                     AKGKVIHWGETSTFLKPHVRKLLSDYDYPNIESTGRTDYGDDVIVLRYADILLMYSEV
                     LNEIQGPSENTLYGVNKVRERVGLSTVKLSVTQEELRDMIFDERKWELAYEGHYYFDC
                     QRTGRLIDEIKRNWHGEREISQEMLTNKYYLMPIPFWAIEKNPSLKQNYGY"
BASE COUNT         1547 a         1228 c          849 g         1687 t
ORIGIN      
        1 gttttctccc aaagctttcc cggaaattaa catcggattg tacacaaaca gggtttcgtt
       61 gcggtacagg gccattccta ccgaaatgac cgagtaggcc ttcaatctct cgggcttcag
      121 ccattgctgg ccataacagg aaatgccgac aaaattggtg tagatatttt cgctgatctc
      181 gccgtaaaac cggaacaccc catcctgcgg atcaaagttt ccatagatac tcgacgaagc
      241 gtaatagaaa tgataatcga cacccaggcc caaatcgggc aaaaacatat agtgcagctg
      301 tccgccggcc tgctccccgg cctgcagctt tttataatac tttttggctt cctgagaggt
      361 aaagcccaca cgttccatct cttgccgggc tgttttggta ttggccgtcc ggatcccata
      421 ccctccgctc agcgctatcc tgaaccggcc gactccccag actggtcggg ccgcctgccc
      481 aaaagcctcc ggctgttcag tcttgctcca ttcccgaaca tcagcccggt caatcctgtc
      541 tttgatcacc acgccattct gcatcctttc aaaatagagg tatttaggtg tcaccccggt
      601 tattttacaa tcgagcgtat cgcccaaaat cataaccatc cggtcctgtg ccgacagaac
      661 ccccgtgaaa aataagaaga acaaaaaaac ggctgagaaa taaatttgtt tttgcatgtc
      721 gttggacgtt tagttgaacg aagttaactt taattaacag tttgcaaaaa aaagaaacgc
      781 ccattttaca tgttgtacaa aacatattga gaaaaaatgc caatcaactg agagaagata
      841 aaatacaccg aaaagccggc cacgttttcc ccgaagtttt tttcggcaaa acccatgacc
      901 ggccttcttc cggaggctgt tgcccctgta atctattacc ttgtttcggg atatcctttt
      961 ttaactatgg tagttgcaaa accccacaag cctatttgag acaaattttg cagtttgctt
     1021 attcggatac gtaataaaaa tgtccatctt ctgcacctat caacagttca ggttttccat
     1081 ctttattcca atcgactatc gtaggacttg tatcatgtcc cgctaatcgt aaatcaaata
     1141 aatctcctct ggacttatag attgtatttc catccttttc gccaatattt tctagtaaca
     1201 caatgttttt cccatcaagt aataaatcaa ggtcaccgtc cttatcccaa tctgtaaaac
     1261 aaaacttacg tcgtccgcta ccacctgctt ctctgttatt taagcgtaat aatccattcc
     1321 cgtgatcaag gtctgcgtct ttaaaaatgc acttcccagg ttggagcaat agctttcctt
     1381 cttttctgaa acgttcaaag aaagtcaggt atccttcgtg gtccaacatt acgagatcca
     1441 ttaatccgtc tttattccaa tctatggcat aaggagttgt tctccattgt gtaactaatt
     1501 catctttctt cggattccac cagttccatg ctggtttagg gattacatca cttctccaat
     1561 cgactttcac ccctcccatt tttgttaatt tcggagatcc ttctctacct acgtttctat
     1621 accattccac tttcccccag atcgaattta caagaatatc tttttgtccg tctccatccc
     1681 aatcagcaac cgaaagagtc gtataacccc atttgcgctc acagggcccc tgtatcgaac
     1741 cattttcacc agccatgata cgaataactt caccatctgc tttaaggtac tcaggtttat
     1801 tccagcgggg agtagttcct ccacccagat tttcaataaa accaatatac ccggcagaat
     1861 ttccgcaaat caaatcttcg tcaccatcgt tatcccaatc tacactgaat ggagtcacta
     1921 atgctccaaa cttgaccaag tctgctttct gcttaaaata gactggagac ttaaatactg
     1981 gcatcccttg atttactgca cctgtgtttt cgatgaaggc gatcctccca tcttcatctc
     2041 caacaatgag gtcaatcttt ttgtctccgt tccagtcaag cgcaacaggc atcatcattt
     2101 ccaagtccat ctgaatcaat cctttggaat tcccaagaaa ttttcctgga gcgtaggcgg
     2161 gcttagtgcg agaaccaata ttctcaaacc aggtaaaccg gtcgacgaac tcgccacaga
     2221 taatatccaa atcaccatcg ccgtcaaaat cctctatgtt cggggaagtg ttgccataaa
     2281 catcaattgg cctatctcca gccatcatct tccctcgaag aacataacgt ccaccctcat
     2341 tttcaagcag gtatatatat ccatgcaaat ctcctcgagt ccattttcct gcactgttaa
     2401 atgcattatc ccagccataa tcagcccaat cgtcgattcc aacgatgagg tccaggtcgc
     2461 catcattttc ataatcgaca tacttccaat gattgaatcg tttcttcgga tgtaacgaca
     2521 aaatttccga tgtatttgaa atccctacag gtttatcata tgcaaacttt atgaaatcgg
     2581 aaaattcaac tccggaacct aatattcttg cttttccgtc aacataggat attgtggtgt
     2641 tagtgattcc ctgtccgatt cttaccggct tacggaaaac agtattagcc gattgctctc
     2701 cacttatatt ttcaaagaaa tatgttccat tatatggcac atcagtacac gaaatgacca
     2761 gatcaatatc tccatccttg tcaaaatcca tcggcagcgg actggaccat aaaccgacag
     2821 ccaaatctac tgttaatcct tcgttgcggt attgcaagcg atgcagctct tctcccttgt
     2881 ccgctttttg agtataactc gttaacccga gtcctacaaa tagtactgat aaaattgtac
     2941 ggcttaaaat atttgttgtc attatttttt atttaagttg tttaaaaaaa atgtggcatt
     3001 tcactttttt aacctatcat actctagttc tctagacttt tgttccaaaa ggacttttaa
     3061 tgttaaatat tcttctcgat tcttatcaat caaattattc ttctcctggg gatcatctgc
     3121 aatattgaat aactgaggtt tgagagaaga ttcttcttcg ggaatatcag tatttataaa
     3181 aggtcttacc aatttccagt ctcccttccg aattgctgca ttatgagtat aataaggaac
     3241 acctctgttc cattgccaaa tccggatttc aggcaattga taatcttcat taaataatat
     3301 tccataaaag cttcccccat caagtgttgc actttcagga attgttaacc cacaaatatc
     3361 tgccagcgtt ggtaatacgt ccgtaaaatg gatcaattca ttacttaacc ctggttttat
     3421 ttgacctgac caattaaaaa taaaaggaac acgaattcca ccttctaaaa cggtatattt
     3481 tgtccctttc aaatcctggt tgaatcgctc tcctgtaatg ggatccggac cattatcgct
     3541 tacaaaaaca ataatagtat tctttctgag tttcattttt tccaattcat ctaataactt
     3601 acctatccca ttatccatta tttcgatcat tgcataaata gtagccgtat ttttattata
     3661 tccttttttt tggtaataat ctatcaacaa gtcaggagcg ctaagtgggc gatgtggagc
     3721 ataatgagca agatgtaaaa aaaatggctt atccttattg cgtttaataa aacctaaagc
     3781 tctatcggtt aaatcatcag taagatactt gtcattgaac tgatggtttt ttccattaat
     3841 attcagtgta tattcaaaat agctttttat taaaaaggga tctataaatc cttcaaattc
     3901 atcaaatccc ctttttaaag gatgatagtc ttctcccata ccgcaatgcc attttcctat
     3961 aagtccggta acatatcctt cattacgaaa ataatcagcc attgtaggta ttcctgattt
     4021 tattcgggaa agctccggga atctttccat atttagcgtt acgcaaccag ttctatgagg
     4081 atattggcct gttaagaggg aggctcttgc tggagcacaa accggggctg ctgaatatgc
     4141 gttattaaag taaagacttt ctacaacgag tttatcaaga ttaggagttt tggtaagtcc
     4201 atgattaaac tttgagatat ctccaatccc catatcatcg gctaaaataa gaataacatt
     4261 gggtttatta tttgcccttt catgaacagt ccgagaatta gcatatccgg agatagtggg
     4321 aaggatagac aaactaattc cgacatattt ccctattctt aaataatttt taatgaattt
     4381 gttatttatc atgtttctgc ctcataaaga taaaattgat tgacttattt tttctcaaaa
     4441 gcattgaagt taaagtagag ttggaaatta gaatccaact ctactatgaa tctttcagat
     4501 taatatccgt agttttgttt taaacttgga ttcttttcta ttgcccaaaa aggtatcggc
     4561 attaaataat atttatttgt aagcatctcc tgactgattt ctctctctcc atgccagttt
     4621 ctttttattt catcaatcag ccgcccagtt ctctgacaat caaaataata atgcccctcg
     4681 tatgctaatt cccattttct ttcatcaaaa atcatatcac gcaactcttc ttgggtaaca
     4741 gacaacttaa ctgtggaaag tccaacacgt tctctaactt tattcacccc atacaaggta
     4801 ttttctgacg gtccttgtat ttcattaaga acttcagaat acatgagaag aatgtcagca
     4861 taacgaagta caataacatc atctccataa tcggttcttc ctgttgactc gatattcgga
     4921 taatcataat cacttagtaa ttttcgtaca tgaggtttta aaaatgttga tgtctctccc
     4981 caatgaatca cctttccctt agcatctgta tattcagtaa ggaaggtagt tttatagcga
     5041 ggattatcaa taccattgta aataataaaa tcattcataa aaacatcgtt agccctagcc
     5101 tgaagaattg aatatggagt tatattagct ccacgaattc cataacgcaa tacaagatca
     5161 ctgccttctc cggattgacc gcattctgca gtactcaaaa actgtattgc aaataatatc
     5221 tcttcattat tgtcatgaac attatcgtat aaccctccaa aaacattttg gaaattttgt
     5281 agtaatgaaa attttcctga atgaataatt t
//
LOCUS       DAOSDR010000228         5267 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrimonas sp. isolate AMR_MDS_1826
            NODE_5574_length_5267_cov_4.649271, whole genome shotgun sequence.
ACCESSION   DAOSDR010000228 DAOSDR010000000
VERSION     DAOSDR010000228.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863355
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrimonas sp. (wastewater metagenome)
  ORGANISM  Petrimonas sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Dysgonomonadaceae; Petrimonas.
REFERENCE   1  (bases 1 to 5267)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5267)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:10:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,720
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 35
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5267
                     /organism="Petrimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5574_length_5267_cov_4.649271"
                     /isolate="AMR_MDS_1826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2023866"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1129)
                     /locus_tag="PLI30_09275"
     CDS             complement(<1..1129)
                     /locus_tag="PLI30_09275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019538904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4301 family protein"
                     /protein_id="HOI79782.1"
                     /translation="MFTKEDLALLRKKNISQQQIDAQLNWLKKGFPFLKILSVASVEK
                     GILRVSKADEEEYRMAWRDSLDAGKKVTKFVPASGAASRMFKDLFTFLDDGNDVPETA
                     FVKTFFNNIGKFAFYDALNETCRNNLGKTVPELIDSGKYKQVVRALLFPDGLNYGNLP
                     KGLLLFHSYEEGNRTPVGEQMTEGTLYAKDKNGVVNIHFTVSEEHKELFELLVAARRL
                     GYELKLGAKFRVSYSVQKPSTDTLAVDMENNPFRDEGGSLLFRPGGHGALIENLNDID
                     SDFIFIKNIDNVVPDRLKESEALYKQLLGGVLVKMQQRAFGYLGELTSGKCSNGKLQE
                     ILKFTENELCIASSNKSFADEEELRDYLIAKLDRPFRVCGMV"
     gene            1352..3274
                     /gene="dnaK"
                     /locus_tag="PLI30_09280"
     CDS             1352..3274
                     /gene="dnaK"
                     /locus_tag="PLI30_09280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012457876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HOI79783.1"
                     /translation="MGKIIGIDLGTTNSCVSVMEGNEPVVIPNNEGKRTTPSVVAFVE
                     NGERKVGDPAKRQAITNPKHTVYSIKTFMGETFDQVQKEIGRVPYKVVRGDNNTPRVD
                     INGQLYSPQEISAIVLQKMKQTAEDYLGQSVSEAVITVPAYFSDAQRQATKEAGEIAG
                     LTVRRIVNEPTAAALAYGLDKQHKDSKIAVFDLGGGTFDISILELGDGVFEVKSTNGD
                     THLGGDDFDHRVIDWLAEEFLKDEGLDLRKDPMALQRLKEAAEKAKIELSSTTSTEIN
                     LPYIMPVNGIPKHLVKTLTRAKFEQLIDDLLQKCVAPCQTSMKDAGLTNSDIDEVILV
                     GGSTRIPAVQALVERLFGKSPHKGVNPDEVVAIGAAIQGAVLTGEVKDVLLLDVTPLS
                     LGIETLGGVMTKLIDANTTIPTKKSETFSTAADNQPSVQINVLQGERSLARDNKQIGV
                     FNLDGIPPAPRGIPQIEVTFDIDANGILNVSAKDKASGKEQKIRIEASSGLSEEEIKR
                     MKEEAAINAEADKKEKERIDKLNQADSTIFQTEKQLKEYGDKIPADKKAPIENALNKL
                     KEAHKAQNIADLDTYMTELNTAFQAASQDMYNAANAQAGAQQAGPQDFSGQQPGGNPS
                     GSQEGDVTDVDFEEVK"
     gene            3569..4660
                     /locus_tag="PLI30_09285"
     CDS             3569..4660
                     /locus_tag="PLI30_09285"
                     /EC_number="1.13.12.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004296139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase family protein"
                     /protein_id="HOI79784.1"
                     /translation="MFKSFFIGEKEIMLPIIQGGMGVGISLSGLASAVANEGGIGVIS
                     SAGLGLLYRRKPGDYLKDCIWGLKEELRKSREKSKGTIGVNIMVALSNFADMVRTTIA
                     EKADIIFAGAGLPLDLPSYLSTSSDTKLVPIVSSARAAKVICEKWHTNYNYLPDAIVV
                     EGPKAGGHLGFKRENLEDANYSLEKLVPEVLAIATSYKDKKTIPVIAAGGISTGEDIL
                     RFIQLGASGVQIGSLFVPTDECDASDAFKQIYVNSSQSDLMIIQSPVGMPGRAFQGDF
                     LDSVSAGNEIPKSCPYHCIKTCDYSKSPYCIIKALYNASKGRMNRGYAFAGANAYLTE
                     KISSVREVISKLKKEFIAAEFLSGKEIAH"
     gene            4746..5171
                     /locus_tag="PLI30_09290"
     CDS             4746..5171
                     /locus_tag="PLI30_09290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008026293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HOI79785.1"
                     /translation="MIKKEFEKKRKEKKQEKQQRREHRKKSNAGKSFEDMIAYVDEFG
                     NITDTPPEPKKQEKVAIESIVISTPKKEEEEITPLTGKVDFFNSDRGFGFIKKTNSGE
                     KYFFHINNAFASIEEGNTVTFDLERGKMGMNAVNITILE"
BASE COUNT         1704 a         1156 c         1102 g         1305 t
ORIGIN      
        1 tcaccatgcc gcatacccga aatggacgat caagtttagc aatcaggtag tccctcaact
       61 cttcttcatc ggcaaaagat ttgttggaag aagctataca aagttcgttc tcggtaaact
      121 tcaaaatttc ctgcaacttt ccgttgctac attttcccga agtcaactct ccaaggtagc
      181 caaacgcccg ttgctgcatc ttcaccaaca ctccacccaa aagttgttta tataaggctt
      241 cgctttcttt taaccggtct ggaacaacat tatcgatgtt tttaatgaat ataaaatcgg
      301 aatcgatgtc attcaggttt tcgatcagcg caccgtgtcc cccgggacgg aaaagcaacg
      361 atccgccctc atcgcggaat ggattattct ccatatccac ggcaagggta tcggtgctag
      421 gcttttgaac gctgtatgag actctgaatt tagcaccgag ttttaattcg taccccaacc
      481 tccgggcagc gaccaacaat tcaaacaact ctttatgctc ttccgaaact gtaaaatgaa
      541 tattcaccac accgttttta tcttttgcgt ataaggtacc ttcggtcatc tgttcaccca
      601 ccggtgttct atttccttct tcgtaactgt gaaacaaaag caaccctttg ggcaggttcc
      661 cgtaattcaa tccgtcagga aagagcaagg ctctcaccac ttgtttgtac ttgcccgaat
      721 caataagttc cggaactgtt tttccaagat tgtttctgca ggtttcgttg agcgcatcgt
      781 aaaaggcaaa cttccctata ttgttgaaaa atgttttaac aaaagcagtt tcaggcacat
      841 cattaccatc atctaaaaag gtaaacaagt ctttaaacat acggcttgca gcacccgatg
      901 cgggtacaaa cttggtgact tttttacccg catccaaaga atcacgccaa gccatccgat
      961 attcttcctc gtcggcttta gatacacgca aaatcccttt ttcaaccgat gcaacactca
     1021 gtattttcag aaaagggaag cctttcttca accaattcaa ctgagcatca atttgctgtt
     1081 gtgaaatatt ttttttccgt aacaacgcta aatcttcctt tgtaaacata ctgtcttata
     1141 ttgtgttgaa aattattttc gattttaaat ctttcacaga aacacttcaa aggtaacgaa
     1201 aaactttaaa tacatatcca cacgtctgtt aaaatattac cgacaatctg tcagtttctt
     1261 ttcaaatatc gttttttggc ataagatttg aaaatgatta aaaagaagct atggatcaga
     1321 aattaatcaa ttaaatacat atatataaat tatgggaaaa ataataggaa tagacttagg
     1381 aaccacaaac tcttgcgttt cggtgatgga aggcaacgag cctgttgtaa tccctaacaa
     1441 tgaaggaaaa cgtaccacac catcggttgt ggcttttgtc gaaaacggag aacgaaaggt
     1501 gggcgacccg gcaaaacgtc aggcaataac caaccccaaa cacaccgttt actccataaa
     1561 aacgtttatg ggtgaaacat tcgaccaggt gcaaaaagaa atcggacgcg ttccctacaa
     1621 agtagttaga ggcgacaata ataccccacg tgtagacatt aacggacaat tgtattctcc
     1681 gcaggaaatt tcagcaatcg tgcttcagaa aatgaaacaa acagctgagg attatctcgg
     1741 acaatccgtt tcagaagccg taatcaccgt gcccgcatat tttagcgatg cgcaacgtca
     1801 ggctaccaaa gaagccggag agattgcggg gttaaccgtg cgccgcattg taaacgagcc
     1861 tacggcagcc gccctggctt acggtctgga caaacagcac aaggactcta aaattgccgt
     1921 gttcgacctg ggaggcggca cgttcgacat ttcgatcctg gaattgggtg acggtgtttt
     1981 cgaggtgaaa tcgaccaatg gagatactca cctgggagga gacgacttcg accatcgggt
     2041 tatcgattgg cttgccgaag aatttttgaa agatgaaggc ctcgacttgc ggaaagaccc
     2101 catggcgtta caacgactga aagaagcggc tgaaaaggca aaaatcgaac tatccagtac
     2161 aaccagcacg gagattaatt taccgtatat tatgccggta aacgggattc ccaaacattt
     2221 agtaaaaacc cttacccgcg caaaattcga gcaactgatc gacgacttgt tgcaaaaatg
     2281 tgtagcaccc tgccagacat caatgaaaga tgccggatta acaaattcag atatcgacga
     2341 agtcatcctt gtagggggct caacccgtat tcctgctgtt caggcattgg ttgaaaggtt
     2401 attcggaaaa tcaccgcaca aaggtgtaaa ccccgacgag gtagtagcta ttggtgcagc
     2461 cattcagggc gccgtcctca cgggagaagt gaaagacgtg ttgttgctgg acgttactcc
     2521 gctgtcgttg ggaatcgaaa ctctgggtgg tgtgatgaca aaacttattg atgctaacac
     2581 caccattccg accaagaagt cggaaacgtt ctctactgcc gccgacaatc aaccttctgt
     2641 acaaatcaac gtattacagg gtgaacgctc tctggcgcgc gacaacaagc aaatcggcgt
     2701 gttcaacctc gacggcattc cacctgcacc gcgtggcatt ccacagattg aagttacttt
     2761 cgatattgat gccaacggca tcctgaacgt ttcggcaaaa gacaaggctt ctggaaaaga
     2821 acagaaaatc cgtatcgaag catcttcggg tttgagcgaa gaggaaatta aacgaatgaa
     2881 agaggaagct gctatcaatg ccgaagccga caagaaagag aaagaacgta tcgacaaact
     2941 gaatcaggcc gactcaacca ttttccaaac cgaaaaacag ttgaaagaat acggtgacaa
     3001 gatcccggca gacaaaaaag cgcctatcga aaacgcgctg aacaagctga aagaagccca
     3061 caaggctcaa aacattgccg acctcgacac ctatatgacc gaactcaaca cagcatttca
     3121 ggctgcttcc caggacatgt acaatgctgc caacgcacaa gcaggggcac aacaagccgg
     3181 tccccaggat ttcagcgggc aacagcccgg aggcaatcca agcggaagcc aggaaggtga
     3241 tgtgaccgac gtcgacttcg aagaggtgaa gtaaagtcga ttaaaatcga taatcaaata
     3301 gtatcaaacc gcagcaaaca gatgatttgc tgcggttttt atttttcaga actacatttt
     3361 ctgaaataac tgaaaatcaa taaaaatgtt gtatctttgc agtctagaca atcaagttta
     3421 tattgatatc cttcacagca atgctttgca ttatcctcat cgggtaagtc ttttcatcgt
     3481 ctaaatcgac ctcaaaatca gctttcttgt ttggatcaac caggtgttca tgtgcaatac
     3541 atcaacatcg ttacacccat ttacagagat gttcaaatca ttttttattg gagaaaaaga
     3601 aattatgcta cccataattc agggcggtat gggtgttggt atatcacttt caggcttagc
     3661 ttcggcggta gccaacgaag gaggaattgg agtgatttca agtgcaggct taggattgct
     3721 ataccggcga aaaccaggcg attatctgaa agactgtatt tggggactga aagaagaatt
     3781 gcgcaaatct cgcgagaagt ccaaaggaac aatcggtgta aatatcatgg ttgctttgtc
     3841 aaatttcgcc gatatggtgc gcaccacaat tgctgaaaag gccgatataa tttttgcggg
     3901 tgcaggattg cccttggatt taccatccta cttatcaaca tcaagcgata caaaactcgt
     3961 tcccatcgta tcatcagcac gtgccgcaaa agtgatttgc gaaaagtggc acaccaatta
     4021 caattatctt ccggatgcca ttgtggtaga agggcccaaa gcaggaggcc acctcggttt
     4081 taagagagaa aatctggagg atgcgaatta ctccttggaa aagctagttc cagaagtgct
     4141 ggcaattgcc acaagttaca aagacaagaa aacaattccc gtaattgctg ccggcggaat
     4201 ttctaccggg gaagatattc ttcgttttat ccagcttggc gcatcaggcg tacagatagg
     4261 aagtctcttt gtgccaaccg atgagtgtga cgcttcagat gcatttaagc aaatttatgt
     4321 caactcctca caatcggacc tgatgattat tcaaagtccg gtgggtatgc cggggcgcgc
     4381 ttttcaggga gattttttag atagcgtcag tgctggcaac gaaataccca aatcgtgtcc
     4441 ttaccactgt atcaagacct gcgattattc aaaaagccca tattgcataa tcaaagcgtt
     4501 gtataatgct tcaaaaggca ggatgaaccg tggatacgca tttgccggag caaacgccta
     4561 tctgacagaa aaaatatcca gtgtaagaga agtgatttca aaactaaaaa aagaatttat
     4621 tgctgctgaa tttctatcgg gtaaggagat agcgcattaa atgactcaaa gaacaaaaaa
     4681 acatcgatta acgaacggtg tacccttatt acaaaccggt ttcggataat cgcaaattaa
     4741 ataagatgat taaaaaagaa ttcgaaaaga aaagaaaaga aaaaaaacag gaaaaacagc
     4801 agcgcagaga acatcggaag aaaagcaatg caggtaaatc atttgaagac atgattgcat
     4861 acgtggacga gttcggaaac attaccgaca ctccacctga acccaaaaaa caagaaaaag
     4921 tcgcgataga gagtatcgtc atttcaacgc caaaaaaaga agaagaggaa atcacaccgc
     4981 tgaccggtaa agtggacttt tttaactccg acagaggctt tggatttata aaaaagacca
     5041 attctggcga aaaatatttt ttccatatca acaatgcttt tgcctcaatc gaggaaggaa
     5101 ataccgttac tttcgatctg gaacggggga aaatgggaat gaacgcagta aatattacta
     5161 tccttgaata gaaaaataat aaagaaacat tattaaaatt aaacaacaat ttaaaaaaca
     5221 agaaaatgaa catttatgta tcaaatttaa gctacgacac aacaacg
//
LOCUS       DAOSDR010000229         5205 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrimonas sp. isolate AMR_MDS_1826
            NODE_5653_length_5205_cov_4.263107, whole genome shotgun sequence.
ACCESSION   DAOSDR010000229 DAOSDR010000000
VERSION     DAOSDR010000229.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863355
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrimonas sp. (wastewater metagenome)
  ORGANISM  Petrimonas sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Dysgonomonadaceae; Petrimonas.
REFERENCE   1  (bases 1 to 5205)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5205)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:10:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,720
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 35
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5205
                     /organism="Petrimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5653_length_5205_cov_4.263107"
                     /isolate="AMR_MDS_1826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2023866"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..598)
                     /locus_tag="PLI30_09295"
     CDS             complement(<1..598)
                     /locus_tag="PLI30_09295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019539975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Wzz/FepE/Etk N-terminal domain-containing
                     protein"
                     /protein_id="HOI79786.1"
                     /translation="MIYEKLKPNDDFIRVQDLFHIFLSKWYWFVLSLFITTALVILYL
                     LITPPIYTRTASILIKDNSKSGTSAGSMAEFDDMGIFRSNTNVNNELITLKSPSLMEE
                     VVTRLDLDKRYISRRGLRKIDLYKSSPVLVMLKNPNGQALQFDIELQPHGKVRLIDFI
                     HSGQALSGKIEGALSDTIATPFGVVMIIPTPYYNEEGGN"
     gene            complement(628..1407)
                     /locus_tag="PLI30_09300"
     CDS             complement(628..1407)
                     /locus_tag="PLI30_09300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006844039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015159 - polysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015774 - polysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis/export family
                     protein"
                     /protein_id="HOI79787.1"
                     /translation="MIVTVALLCSCVSVKNINYFQDLEPGVAEQTGAPLEIKIRSNDK
                     ISIVVNSKDAELSNLFNLPIVSHRIGASTGQSLSGNAQQVSGYTVDMYGDIDFPVVGK
                     VHVAGLTRAEIATHIKHTLITEDLIKDPVVTVEFMNLGFSVLGEVNNPGKFYIDRDGM
                     TLLDAIGMAGDLTIYGKREAVYVLRDEQGVQVPYRIDLTSARQMYFSPAFYLQQNDVV
                     YVEPNSVRARHSTVNGNNVRSTSFWISLASLLTTITVLFFK"
     gene            complement(1475..2527)
                     /locus_tag="PLI30_09305"
     CDS             complement(1475..2527)
                     /locus_tag="PLI30_09305"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019539977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MraY family glycosyltransferase"
                     /protein_id="HOI79788.1"
                     /translation="MKKALFDSHDERKIHSGVVPRLGGVAFFPALLFPFLVILSINLL
                     LQTSLVVSVNITLYIELMLALSAMLLIYFTGVGDDLMNIRYRIKLMIQIMTGILFTSS
                     GVWINNFYGLFGLNEINAAIGIPLTVVLIVFIINSINFIDGVDGLAAGIGMFAFLCYG
                     FIFLYMQSYVYSLLAFSSFGVLIPFFYYNVFGKVNRGTKIFMGDSGSLTIGMLLSVFA
                     VKVCHYNPGSLFQSSQSIVLAFSILLVPMFDALRVILLRICTGKSPFKPDKRHLHHQF
                     LQLGFSHRGIMLMILTIAAFFGTGSMLLAPYVDINLLLGLIILLWASMDIYLRIKIKH
                     AAQSISAKSKRIIKQV"
     gene            complement(2670..3194)
                     /locus_tag="PLI30_09310"
     CDS             complement(2670..3194)
                     /locus_tag="PLI30_09310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019539978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UpxY family transcription antiterminator"
                     /protein_id="HOI79789.1"
                     /translation="MKASDTSYWYALRVTYSREMMVKKHCDANNIINFIPLEYRMCER
                     NGVIIKKLQPAIRNLIFVKTSMSCINELKRKYPIRYIMNRGTGDPVIVPEKQMLDFIA
                     VAGNYDQQVVFLAPCELRTKTGSRVRIRDGIFKGVEGVLVRVRNNKRVVVQIEGLVMV
                     ATHYIHPSLLEYLE"
     gene            complement(3676..4887)
                     /locus_tag="PLI30_09315"
     CDS             complement(3676..4887)
                     /locus_tag="PLI30_09315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009317092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HOI79790.1"
                     /translation="MTTIRVKLRTSTVSGKAGSIYYQVIHCRKHVQLTSSVKLYPHEW
                     DAHHACVHTNGERTSFLLAAQRTINADLAVLHDIISRKMAMGTPYDAADIALSFRMLT
                     PVEEDFFSYMEKRIRKLEEENAHGTKLNYWRAYRSFATFLGKQYLPFPACTEMLIGEY
                     EKWLRQRKIKRNSSSFYMRILRAVYNLAVSDELTSTENPFRKVYTGIDTTRKRAVDKH
                     VIRQLKTLDLTENPTLAYARDLFLFSLYLRGMAFVDMAYLQKCDIRRGDVTYIRRKTG
                     VRLCVKMEPCLQKIVERYEEKTAHTLYVLPIITSVDEKQAYREYQNALSYYNKQLKRL
                     SEMLGYNIPLSSYTPRHTWATTARDSNIPLSVISAGMGHSSENITRIYLSSLDNSIID
                     KANCKILSVFD"
BASE COUNT         1610 a         1070 c         1024 g         1501 t
ORIGIN      
        1 gattaccccc ttcctcattg taatagggtg tagggatgat catcaccact ccaaaaggag
       61 tagcaatggt atcggacaaa gcgccttcta ttttaccgga aagagcttgc ccagaatgaa
      121 taaaatcaat taatctaact ttcccgtgag gctgcaattc tatatcgaac tgcagggctt
      181 gcccattggg atttttaagc atcactagta ccggcgaact tttatacaga tcgatttttc
      241 tcaaaccacg tcgggaaata taccttttat ccagatcgag ccgggttacc acctcttcca
      301 tcaatgaagg cgatttcaat gtgatcaact cattattgac gttggtgttg gacctgaaaa
      361 tacccatatc atcaaattcc gccatgcttc cagcggatgt cccgctcttg gagttgtctt
      421 taatcagtat ggaagcggtg cgggtgtata tgggcggagt gattaacaga taaaggatca
      481 ccagtgccgt agtgataaat aaactcagta caaaccagta ccatttggac agaaatatat
      541 ggaacaggtc ctgaacccta ataaaatcat cgtttggttt cagtttttcg tagatcatat
      601 agttaaatga atagtgaaaa ctaaaaatta tttgaaaaaa agaacagtaa tggtggtgag
      661 aagagacgcc agggatatcc agaatgaagt ggaacgcacg ttatttccat tgacggtgga
      721 atggcgcgcc cgtacgctat taggctctac atataccaca tcgttttgtt gtagatagaa
      781 tgccggagaa aaatacattt gcctggcgga agtaaggtct atacggtacg gtacctgaac
      841 tccctgttcg tcgcggagca catacactgc ctcacgtttt ccgtagatgg taaggtcgcc
      901 cgccataccg atggcatcca acagggtcat tccgtctcga tcaatataaa acttgcccgg
      961 attgtttact tcacccagca ccgaaaaacc caggttcata aattcaacgg taactaccgg
     1021 atcttttatc aggtcttcgg tgatgagcgt atgttttata tgggtggcaa tctccgcacg
     1081 agtaagcccc gccacatgga ccttacccac gaccgggaag tcgatatcac catacatatc
     1141 taccgtataa cccgatactt gttgtgcgtt gccggacaag gattgcccgg tggatgctcc
     1201 gatccggtgt gaaacaatag ggagattaaa aaggttggat aattccgcat ctttactgtt
     1261 cactacaata gaaattttat cattagaacg tatctttatc tctaaaggtg cgcccgtttg
     1321 ttccgcgact ccgggttcca ggtcctggaa atagttgata ttttttacgg atacacacga
     1381 acacaagagg gcaaccgtaa cgatcatcga aaaaatgttg taagtctttt tttgtttcat
     1441 ttgataaaat tgttaacagg ttgttattgg gcagctacac ttgtttgatt attcttttgc
     1501 ttttggcaga aatagattga gccgcgtgtt ttatttttat cctcaaatag atatccatac
     1561 tggcccataa taggataatc aatcccaata gcaggttaat atcgacatat ggagccagca
     1621 gcatactccc cgttccgaaa aaagcagcga tcgtcaggat catcaacata atacccctat
     1681 gggaaaaccc caactgcaga aactgatgat gtaaatgcct tttgtcgggc ttgaaaggac
     1741 ttttgccggt acagatcctc aataagatca ccctcagcgc gtcgaacatc gggacgagca
     1801 atatggagaa ggccaataca atcgattgcg acgactgaaa aaggctcccc gggttataat
     1861 gacatacctt aacggcaaaa actgagagaa gcataccgat cgtaagcgat cctgaatctc
     1921 ccataaatat tttagttccc ctgttcacct ttccgaatac attataatag aaaaatggga
     1981 ttagcacccc aaaagaactg aatgccaata aactataaac atatgattgc atatagagaa
     2041 agataaaccc atagcatagg aaggcaaaca ttccaatccc ggctgccaga ccgtcaacac
     2101 catcaatgaa gttgatggaa ttaataatga aaacgataag aacaacggta aggggaatac
     2161 cgatggcagc gtttatctcg tttaacccga aaaggccgta aaagttattg atccatactc
     2221 cggaggaggt aaataatatt cctgtcataa tctggatcat cagttttatc cggtagcgga
     2281 tattcatcag gtcgtccccc actcccgtaa aatagataag cagcatggca gacaaagcca
     2341 gcatcagttc gatgtataac gttatgttaa ccgaaacgac caaggatgtt tgcagtagga
     2401 ggttgatact gagaatcaca agaaagggaa ataacagggc ggggaaaaaa gcgacccctc
     2461 ctaacctcgg aacaactccc gaatgtattt tcctctcgtc gtgtgaatcg aacaatgcct
     2521 ttttcataca gatttgcaga atcttgggaa ggagtatcca tgaaaaaaat gcactgacaa
     2581 cggatacaga gaaaaaatta acaaataatt ccatctttta ggtattatat gctttatagg
     2641 cgacattaaa acagcgtatc gatgctctat tattccaaat attccagcaa agaaggatgt
     2701 atataatgcg tggccaccat taccagtcct tctatttgca caactacacg tttattgttc
     2761 ctgactctta ccaacactcc ctcaactcct ttgaatatac cgtcccttat gcgcaccctg
     2821 ctgcctgtct ttgtccttaa ttcgcaggga gctaaaaaaa cgacctgttg atcgtaattg
     2881 ccggcaacgg caataaaatc gagcatttgc ttttcaggaa caatcaccgg atcgccggta
     2941 cctctattca taatgtaacg tatcggatat ttccttttta attcattgat gcaagacatg
     3001 ctggttttca caaagatcaa atttcgtatc gcaggttgca actttttgat tataacaccg
     3061 ttacgttcac acattctata ttccaacggt ataaagttaa tgatgttgtt agcatcacaa
     3121 tgctttttga ccatcatctc cctgctatag gtaacccgta atgcatacca atatgaagta
     3181 tcactggctt tcatgttttc atttcgtata aggacatttt ccggggacac caatatttat
     3241 aaaacactga tttatagatt atctatatcc acccaaaaga taaagtacta tttctgctcc
     3301 agaaacagga ctttatctct tacaaagata tgaaagaaat ctgaactcaa gttacaaaat
     3361 attacatttt ttatgtaaag aaaagtttgt tttgagtgtt tatatctgat aactaacagt
     3421 ttatgtttat aaaagtatgt cgataaataa tattaattca agggctgagc gttaatgatc
     3481 ttttacaaat ggttttttta tacttcgtaa tattttttta gagaaaagaa ttattctaaa
     3541 aataatgggt tttgttatat aaaacgaaac gatagttctt atttatttta ttgttaatca
     3601 gaatatcaaa tctgtttatc ctttctttcc tgtttctgga gcagaataag gctttttttt
     3661 accacctaaa gcaacttaat caaacaccga caatatttta caatttgctt tatctatgat
     3721 cgagttatct aaggaagaga gataaatccg tgttatattt tcggatgaat gtcccatgcc
     3781 tgcgctgata accgacaaag ggatattact gtcgcgcgct gttgtcgccc aggtatgacg
     3841 aggcgtataa gacgaaagcg gaatgttgta ccccaacatt tccgacaacc ttttaagttg
     3901 cttgttgtaa taactcaaag cgttctggta ctcacggtac gcctgttttt cgtcgacgct
     3961 cgtaataata ggcaaaacat aaagcgtatg ggctgttttt tcttcgtagc gctcaacaat
     4021 cttctgtaaa cagggttcca ttttaacaca tagacgtacc ccggtttttc tgcggatata
     4081 agtcacatcc cctcgccgta tgtcgcattt ttgcaaataa gccatgtcca caaatgccat
     4141 tcctcgcaaa tagaggctga aaagaaagag atcgcgggca taagctaaag tcggattttc
     4201 tgttaggtcc agagttttta actgcctgat gacatgtttg tccaccgcac gtttacgggt
     4261 agtgtctata cctgtatata cttttcggaa aggattttct gtagaggtca attcgtcaga
     4321 caccgccaaa ttataaaccg cacgcaggat acgcatataa aatgagctac tgtttcgttt
     4381 tattttgcgc tgccgcaacc atttttcata ttcaccgatg agcatttcgg tacatgcggg
     4441 aaacggtaga tactgcttgc ccaagaacgt cgcaaaactg cgatatgccc gccagtaatt
     4501 gagtttcgtt ccatgagcgt tttcctcttc cagcttacgt atacgttttt ccatgtatga
     4561 gaagaaatct tcttctacag gcgtaagcat ccggaacgat aaagcgatat cggcagcatc
     4621 ataaggagtg cccattgcca ttttccggga aatgatgtca tggagaacag ccagatcggc
     4681 atttattgtc cgttgcgccg ctaacaggaa agaagttctc tcaccgttgg tatgaacaca
     4741 tgcgtggtgt gcatcccact catgaggata cagttttacg gaagaagtca actgaacatg
     4801 ttttctacaa tgaataacct gataataaat cgatcctgct ttacccgata cggttgatgt
     4861 tcttaatttt acgcgaattg ttgtcatagc ataatttttt taaattaaaa gaaatagtgt
     4921 gaataaatta aaaagactca tcctatatta taatgaaaag gtaaagcatt ttcaaattgg
     4981 aacgaattaa tctagcttat atacgctgaa gctgtaatga tagatacagc cagcattaat
     5041 taacccactc atgtagaaat tgaatcttac gtattcaaga tgacttgtaa aaatatgtat
     5101 gatgcagtag ccaggctcta gattgatata aggaaagacc tgtgggggtg gaagtaggaa
     5161 atcatacaaa aaaagggaac aaaaaattat gaaaccacaa ttaag
//
LOCUS       DAOSDR010000230         5204 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrimonas sp. isolate AMR_MDS_1826
            NODE_5655_length_5204_cov_4.093999, whole genome shotgun sequence.
ACCESSION   DAOSDR010000230 DAOSDR010000000
VERSION     DAOSDR010000230.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863355
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrimonas sp. (wastewater metagenome)
  ORGANISM  Petrimonas sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Dysgonomonadaceae; Petrimonas.
REFERENCE   1  (bases 1 to 5204)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5204)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:10:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,720
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 35
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5204
                     /organism="Petrimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5655_length_5204_cov_4.093999"
                     /isolate="AMR_MDS_1826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2023866"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            112..738
                     /gene="recR"
                     /locus_tag="PLI30_09320"
     CDS             112..738
                     /gene="recR"
                     /locus_tag="PLI30_09320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006843080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HOI79791.1"
                     /translation="MNSQYPSALLENAVNEFAKLPGIGRKTALRLVLHLLRQEDSQVE
                     EFAQALVTLKQDVKYCRNCHNISDSEVCGICSDKSRDASTLCVVENIREVMAIENTTQ
                     FKGLYHVLGGIISPIDGIGPSDLQITGLEERVKQGGVKEVILALSATMEGDTTNFYIF
                     RKLHPYDVKVSIIARGVAVGDEIEYADEITLGRSILNRTPFDESYKLK"
     gene            735..1169
                     /locus_tag="PLI30_09325"
     CDS             735..1169
                     /locus_tag="PLI30_09325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI79792.1"
                     /translation="MTKTIRELNRHYYISIVVLAVFFLIVIFRVIGFGTPVRVTMTAE
                     MYAIGITLLLIPVALKLFAEKVKKIPKGTGKARTMKYYKDAWLLRMYIVNAVTAGNIF
                     LYALSQSKNFIWLAVISFIVYMFCKPSCQELETVVGESEGVK"
     gene            1166..1693
                     /locus_tag="PLI30_09330"
     CDS             1166..1693
                     /locus_tag="PLI30_09330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019538489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOI79793.1"
                     /translation="MRDFFIRDTLFLRPLEPEDLDLLYQWENDTRLWKNGSTLTPFSR
                     FALRQYLADARQDIYQARQLRMMIVEKESNKAAGTIDLYDFDPLNGRAGVGILVDENF
                     RKKGHALQALLCMEDYAFSHLKLRQLYAFVPAGNIPSRALFEKANYEETAVLRDWISL
                     NRSFTDVIVMQKINK"
     gene            1698..2261
                     /locus_tag="PLI30_09335"
     CDS             1698..2261
                     /locus_tag="PLI30_09335"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005677067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HOI79794.1"
                     /translation="MQEDIKKACEVLRKGGVILYPTDTIWGIGCDATNEDAVKRVYEI
                     KKRDDSKSMLVLMENPVKLQNYITDVPDIAWDLIDLADKPLTIIYDGARNLAANLIAE
                     DGSVGIRITSELFSAELCKQFRKPIVSTSANLSGEASPSTFKEISEEIKNAVDYIVTY
                     RQKDKNKAQPSGIVKLSKNGTISIIRK"
     gene            2271..4061
                     /locus_tag="PLI30_09340"
     CDS             2271..4061
                     /locus_tag="PLI30_09340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019538491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HOI79795.1"
                     /translation="MIVSEIYNKIHNWRKINIKERHFLLFACFIVGILTALAAYLLKL
                     SIHFVELFLTNNFSQTGANYLYLVYPIIGILLAGLFVKYWVKDDISHGVTKILFSISQ
                     RKSRIKPHNMYTSLVASSITIGFGGSVGAEAPIVLTGSAIGSNVGRFFKLDQRNLMLL
                     VGCGAAGAIAGIFKAPIAGILFVIEVLLLDLTLSSILPLLVTAVTATTISYILTGTDA
                     MFAYSQVEAFTLQRIPYVILLGIICGFLSLYVTRTMSWSEDVFSRLKPWTRFALGGVS
                     LSILIFLFPPLFGEGYNSIETLLAGGHKFNELLNGRFFYNAQSRWGIVVFLGLILFFK
                     TFATSATNGGGGTGGVFAPTLFMGCILGFIYSYTMNELGLVTYLPQENFALMGMAGVM
                     AGVMHAPLTGTFLIAELTGGYELFLPLMIVSICSYATIVVFEKHNIYAMRLAKRGELI
                     THHKDKAVLTFLKVEDMLETNIPIVHPEMTLGDMVKVISSSQRNIFPVVNDDGTLQGL
                     VLLNDIRNIMFRPELYDTFKVKKFMVGSPAEIKIDTPMEKVMNTFEDTKAWNLPVVDD
                     NGIYKGILSQSSVFNYYRDVLVENYSEEEE"
     gene            complement(4073..>5204)
                     /locus_tag="PLI30_09345"
     CDS             complement(4073..>5204)
                     /locus_tag="PLI30_09345"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOI79796.1"
                     /translation="YNMEPNGHWAVRAWDVAATEEGSSGSPLLDKEKRVLGALTGGES
                     QCSSPPGPDVYSSLYKAWNITGSLGNPNSLKSYLDPGNSQFQQIDGFNPYLNQPYTRS
                     SNYVATDSVIQSYYNSVPMFATNNTFGYNEFAEEFYASTPARLNGVFVTSPITNNIQN
                     LNIRIKIYADNNGSPGTLLHETSLSYSFRYYQAGDFYETYRDMRNNVENYVKFSDPVE
                     VSGKFFISYTDVSPVPNGFSLLNVQPRKIGSGIVSTAWMKNATGWVRSSENIENPINT
                     SLLITPYVIGTGLTAPDDPSQKETEIKAYYNKAVNRIFIESNKDLIAWEIFYVSGQKV
                     FEEKAESSINRASFSVGHLAKGVYIVKVNVDGINKSLKVLVR"
BASE COUNT         1400 a         1051 c         1252 g         1501 t
ORIGIN      
        1 cttcgctcct cgattttcaa ttttgcgctc ggcaaatcaa ataaatttgc tctgcactcg
       61 cttaacaaaa atgttcgtat atttgtggtt tgaattagct tattaaaatt aatgaactcg
      121 caatatcctt cagctttact agaaaatgca gtcaacgaat ttgcaaaatt gcccggtatc
      181 ggcagaaaga cagcactaag gcttgtttta catttgctaa ggcaagaaga ttcacaggtt
      241 gaggaatttg cgcaagccct ggttacatta aaacaggacg taaagtactg ccgcaactgt
      301 cacaatattt ccgacagtga agtctgtggc atctgctccg acaaatcgag agatgcttca
      361 accctttgtg tcgtagagaa tattcgcgag gtgatggcca ttgagaatac tactcagttc
      421 aaagggttgt atcacgtgtt gggaggaatt atttcgccca tcgatggaat aggcccgtct
      481 gacctgcaaa ttaccgggtt ggaagaacgt gttaaacagg gcggtgtgaa agaggtgatt
      541 ttagcactga gtgcgaccat ggagggagac acgaccaact tttatatttt ccggaagcta
      601 cacccttacg atgtgaaagt gagcattatt gcgcgaggtg tagcggtcgg tgatgagatt
      661 gagtatgccg atgagataac gttgggcagg tctattttaa acaggacacc atttgacgaa
      721 tcgtataaat taaaatgaca aaaaccatca gggagttaaa caggcattat tacatcagta
      781 tagttgtttt ggccgttttc tttttaattg tgatctttag ggtcataggc tttggaacac
      841 cggttcgcgt aacgatgact gccgaaatgt atgccatcgg gataacgtta ttgctcattc
      901 ccgtcgcact taagctgttt gccgaaaaag taaagaaaat tccaaaggga accggcaagg
      961 ctaggacgat gaagtactac aaagatgcct ggttgttgag aatgtatatc gttaacgcgg
     1021 tgacggcggg aaatattttt ctatatgccc tgtcgcagag caaaaatttt atctggcttg
     1081 cggtaatttc ttttattgtt tatatgtttt gcaaaccttc ttgtcaagag ttggaaaccg
     1141 ttgtcgggga aagtgaaggc gtgaaatgag agactttttt atcagagaca ccctcttttt
     1201 aaggccgttg gaacccgaag atctcgatct tctttaccaa tgggaaaacg acaccaggct
     1261 gtggaaaaac ggatctaccc taacaccttt ttcccgattt gccctccggc aatacctggc
     1321 ggatgcccgg caagatattt atcaggcccg gcaacttcgg atgatgattg tggagaagga
     1381 aagtaacaag gcggcaggca cgattgacct gtacgatttt gatcccctga acggccgggc
     1441 cggagttggg attttggtgg acgaaaattt tcgaaaaaaa ggacatgcgt tgcaggcatt
     1501 gttatgcatg gaggattatg ccttcagcca tttaaaactc cgccagcttt acgcttttgt
     1561 ccctgcagga aatataccaa gccgggcctt gtttgaaaaa gcgaattatg aagaaactgc
     1621 cgttctgcgg gactggattt ctctaaaccg ctcgtttacc gatgtaatag taatgcaaaa
     1681 aattaacaag taagtgtatg caggaagaca ttaagaaagc atgtgaagtg ttgcggaagg
     1741 gcggtgtgat tttgtaccct accgacacga tctggggaat cggatgcgat gccaccaacg
     1801 aagatgctgt aaaacgggtt tacgaaataa agaaacgaga cgattcaaag tcgatgctgg
     1861 tgttgatgga aaatccggta aaactgcaga attatatcac ggatgtccct gacatagcgt
     1921 gggacctgat agacctcgcc gacaaaccgc ttaccattat atacgacggc gccagaaatc
     1981 tggcagccaa ccttattgcg gaagacggat ccgtcgggat ccggattacc tcggaactct
     2041 tttctgccga actttgcaaa cagtttcgca agcctattgt gtctacatca gccaacctaa
     2101 gtggagaagc ctcaccttca actttcaagg agatttcgga agagataaaa aacgcagtgg
     2161 attatattgt cacttaccgg caaaaagaca agaacaaagc ccaaccttcg ggtattgtta
     2221 aactgtctaa aaacggcaca atcagtatta tccgaaagta atttagtagt atgattgtca
     2281 gtgaaatata caataaaata cacaattgga ggaagataaa catcaaggaa cgccatttct
     2341 tgctgtttgc ctgttttatt gtaggtattc ttaccgcact ggcagcttat ttgctgaaat
     2401 tgtccatcca ttttgtagag ttgttcctta cgaataattt ttcgcaaacc ggagccaatt
     2461 atttgtacct ggtttatcct atcatcggga ttttgctggc cggattattt gtcaagtatt
     2521 gggtcaaaga cgatatcagc cacggagtaa caaaaattct tttctctatc tcacaacgaa
     2581 aaagccggat caaacctcat aacatgtaca cctcgctggt agcaagttcc atcactattg
     2641 gttttggcgg atcggtagga gcggaggctc ctattgtgtt aacaggatcg gctatcggat
     2701 cgaatgtggg gcgatttttt aaactcgatc agcggaacct gatgttgctt gtaggttgtg
     2761 gggctgcggg cgccatagcc ggaatattca aggctcctat tgcgggaatt ctttttgtga
     2821 ttgaggtgct tttgcttgat ttaacgttat cgtctatact tccattgttg gtaaccgctg
     2881 ttacggccac taccatatcg tatatcctta ccgggacgga tgcgatgttt gcctactcgc
     2941 aggtggaagc ttttaccctt cagcggattc cttatgtaat actgttgggg attatttgtg
     3001 ggtttctctc gctttatgtc acccgaacca tgagctggag cgaggatgta ttttccaggc
     3061 tgaaaccctg gacacggttt gctttgggcg gtgtctccct cagtatactt atttttctgt
     3121 ttccgccttt gtttggagaa ggatataatt ccattgagac gctcttggcc ggaggccata
     3181 aattcaatga actgttaaac ggtaggttct tttataacgc ccaaagccgg tggggaatag
     3241 ttgtttttct aggattgata ttattcttta aaacctttgc aaccagtgct accaacggtg
     3301 gaggaggtac cggaggagtc tttgcgccca cgttgtttat gggttgtatt ttgggcttta
     3361 tctattctta tacgatgaac gaactgggat tagtgactta tcttccgcag gaaaattttg
     3421 cactgatggg gatggctggg gtgatggccg gagtaatgca tgcaccgctt accggaacgt
     3481 ttctcatcgc agaacttacg ggagggtatg agctgtttct accgctgatg attgtctcta
     3541 tttgctctta tgcgaccata gtagtttttg aaaaacacaa tatttacgca atgcgtctgg
     3601 cgaaaagagg tgaactgatc acgcaccata aggataaagc agtcctcact ttcctgaagg
     3661 tcgaggatat gctggaaacc aatatcccta ttgttcatcc cgagatgacg ttaggcgata
     3721 tggtaaaagt gatatcaagc agccagagaa atatctttcc ggtggtgaac gatgatggaa
     3781 cactacaagg gcttgtgttg ttgaacgata tccggaatat tatgtttcgt ccggagcttt
     3841 acgatacttt caaggtgaag aaatttatgg taggttcgcc ggcagagatc aagatcgaca
     3901 ctccgatgga gaaagtgatg aatactttcg aagatactaa agcatggaac ttaccggttg
     3961 ttgatgacaa tggaatttac aagggaattc tctcacaatc atctgttttt aattactacc
     4021 gcgatgtatt ggtggaaaac tactccgaag aggaggagtg agtaaaatcc ggttatctta
     4081 ccaacacctt taagctttta tttattccgt caacattcac ttttacgata taaacccctt
     4141 tggccaaatg accaacggag aaggaggctc tgttgatact gctttcggcc ttttcttcaa
     4201 agactttttg tcctgataca tagaaaattt cccacgcgat cagatctttg tttgattcaa
     4261 taaaaattct gtttaccgct ttgttgtagt aggcttttat ctctgtttct ttttgtgaag
     4321 gatcatccgg ggcagtcagt cctgtaccaa tcacgtaggg ggtaattagc aacgaagtgt
     4381 tgatgggatt ttcgatgttc tcggaagacc ttacccaacc cgttgcattt ttcatccacg
     4441 cagtggatac gattcccgat ccaattttcc tgggttgtac attcagcagg gaaaatccat
     4501 tgggtaccgg gcttacatcg gtgtatgaga taaaaaactt tccggacact tccactggat
     4561 cagagaactt tacgtagttt tctacgttgt tcctcatatc gcggtaagtt tcgtagaaat
     4621 ctccagcctg ataataacgg aacgaatagc tgagcgatgt ttcgtgcagg agtgtaccgg
     4681 gcgatccgtt gttgtcagca tagattttga tccgaatgtt taggttttga atgttgtttg
     4741 tgatgggaga agtaacaaaa acaccattga gccgggcggg tgtggatgcg taaaattctt
     4801 cagcaaactc gttatatcca aacgtgttgt ttgtcgcaaa catcggcaca gagttgtagt
     4861 acgattgaat aacggaatcg gtggccacat aattggaact ccgggtgtag ggttggttca
     4921 ggtagggatt gaatccgtca atttgttgaa actgggagtt tcccgggtcc agataacttt
     4981 tcaatgaatt cggatttcct aacgaaccgg taatgttcca tgccttgtat aacgatgagt
     5041 agacatcagg gcctggggga gacgagcatt gcgactcacc accagtaagt gcccccagga
     5101 ctcttttctc cttgtccagc aagggagagc ccgacgatcc ctcttccgtt gcggccacat
     5161 cccatgcgcg aacagcccag tgcccgttgg gttccatgtt gtat
//
LOCUS       DAOSDR010000231         5201 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrimonas sp. isolate AMR_MDS_1826
            NODE_5661_length_5201_cov_2.173921, whole genome shotgun sequence.
ACCESSION   DAOSDR010000231 DAOSDR010000000
VERSION     DAOSDR010000231.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863355
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrimonas sp. (wastewater metagenome)
  ORGANISM  Petrimonas sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Dysgonomonadaceae; Petrimonas.
REFERENCE   1  (bases 1 to 5201)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5201)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:10:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,720
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 35
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5201
                     /organism="Petrimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5661_length_5201_cov_2.173921"
                     /isolate="AMR_MDS_1826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2023866"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..247
                     /locus_tag="PLI30_09350"
     CDS             <1..247
                     /locus_tag="PLI30_09350"
                     /inference="COORDINATES: protein motif:HMM:NF019011.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="M81 family metallopeptidase"
                     /protein_id="HOI79797.1"
                     /translation="SFGYCMLMHGRVERSVFDNYKNEILNEVRNARKLDAIYLGMHGA
                     EGNKVVVLKTLHSFVEIRDFKEAGLNPLDRNMTWLAK"
     gene            530..943
                     /locus_tag="PLI30_09355"
     CDS             530..943
                     /locus_tag="PLI30_09355"
                     /inference="COORDINATES: protein motif:HMM:NF020376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIRAN domain-containing protein"
                     /protein_id="HOI79798.1"
                     /translation="MWAAFLFSGKEIKSKNLLYDLKKVNDTVLLERELENKYDRFAIK
                     MLKDGRFLGYIAAYENIVMTMLMDQGVQLGASVSSVTENINETRYLDKVFSVQLFTKL
                     PVPYNYLEINNLRTKRADDAVAIYRKGTLINKEKL"
     gene            1197..>5201
                     /gene="cas9"
                     /locus_tag="PLI30_09360"
     CDS             1197..>5201
                     /gene="cas9"
                     /locus_tag="PLI30_09360"
                     /inference="COORDINATES: protein motif:HMM:TIGR01865.1"
                     /note="Cas9, originally named Csn1, is the large,
                     multifunctional signature protein of type II CRISPR/Cas
                     systems. It is well known even to general audiences
                     because its RNA-guided endonuclease activity has made it a
                     popular tool for custom editing of eukaryotic genomes;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0004520 - endodeoxyribonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II CRISPR RNA-guided endonuclease Cas9"
                     /protein_id="HOI79799.1"
                     /translation="MKTILGLDVGTNSIGWALIEHGPENRKGRIIGMGSRIISMGAEL
                     SNFERGQAQTKNANRRIARGARKLNKRYKQRRNKLIYALKELGMLPNQIQLVKDFFDP
                     NKIDKISIKPIKKRQPQLTAFDLLELRVKALSEKIELKDLGRIIYKYNQLRGYAGGNA
                     EPDKEDNNDDAEYQEKKNENYIAFARVLQLGIPEEVTIKNRKFNKRKISIETDDGILD
                     GETYLDSLKENDSLELQVNIRRSKSGESITVKLPDKTNWRKKMENLEKELKEKSKELG
                     REVYISEHLFDIVKENPRAKIRNNVILRSRYESEFDAIWDSQYKVNEDFKRLVDNKKL
                     LTKILCFIFPGTSKTQEQFRAEGLEKGLKYVLKNQIIFFQRELKDQSHLISDCRFEPK
                     EKVAPKSHPIFQEYKIWEQINKLLINTKTEVGVNRKGEQKYTYIDRPIPASLKVKLFD
                     DLQNKKEISYGVIYNELKKKYELREKVDFLNGLNVKAKLKGNETRIMLQKSLGNYLWE
                     ALNLDAIERQTELWNILYHGKGNEYDIHSERTSRVLRFLKEYGPGIDNVEDTAIRISK
                     IKFSRSYSNLSLKAIEKLLPLVRAGKYFDNAFSNEIHSRIVKLLNENVEDPFEKAAQE
                     FLEKNSMVLEEGGIVNAMAVILVYGKHTAKEFTEDELVKTPTGISFIKNGSLRNPLAE
                     QIINETLAMVKDIWRKYGGKPDEIRVELARELKSSAEERAKIYSVNQKNQKANEAVRE
                     KLIELKEELSIANIEKYKLWLSQENLQEEYIKHYSDPSKSEVEKLKLWEEQGHVSPYT
                     GQPIPLSHLFNKARYDIDHIIPKSRYFDDSFTNKVICEKAVNTDKGNRTAMEYFETGS
                     MTQKIFSKDDFIDHANKLFTGRKRKNLLATKVPEDPIQRQIKDTQYIAVKVREELNKI
                     VGNENVEATSGGVTDYLRNHWGLTDKFKSMLKERYDNIQPKLSEIAYNEYVKNIQTKQ
                     KEYAEKNIPFNDIVLNNELFVEKFNKEFFKKKNNKLVINGWSKRIDHRHHALDALIIA
                     CTQPPHIKRLNDLNKVLEDWLKKNKETFLPDFNGSKAELLDEVLNLDENKRKSIFKEL
                     DKFTDIQIPWQGFVEEAEEGLKQIIVSHKPKDKLLLQYDEKGNRQIKLRGQLHEGTLY
                     GKTNGRESYRIPLLKLAGKNFATEKIIEKIVDPFLKKQIREHLNQYKGKKDEAFSAEG
                     ILDLNNKLAERRGKNDNPAPHTPVSTIKIYYKDPDKNKNANDEEIITLQKLDRSKAFN
                     NSLYVKTGDNYLFAVMEKDYFNKKTRQTEKKRVFDLITFFDAANLLKGEFNKSSDKNT
                     FDKDLLFKKYFEEKN"
BASE COUNT         1970 a          876 c         1048 g         1307 t
ORIGIN      
        1 ttcattcggg tattgcatgc ttatgcatgg ccgggtggaa cgctccgttt ttgataacta
       61 taaaaacgaa atcctcaacg aagtaaggaa tgcgagaaaa cttgatgcca tttacctggg
      121 tatgcacgga gcagagggaa ataaagtcgt agtcttaaag acgctccatt cgtttgtaga
      181 gattcgtgat tttaaggaag ctgggctaaa tcctttggac aggaatatga cctggttagc
      241 aaaataaatt ctatgaacaa tggctgaagg ctacattggc cattcttgtc tgacaaagaa
      301 aaaggcgggt taccccgcct taaatatttt cacaacggaa taatccttat tgtgaattgc
      361 tttcaatagt gagcaaagat accaatttgt tgtcagatgt gcaacttttt cagaaaaaaa
      421 agttaacttt accccatatt attctaatac attttattac agtaaattaa aaatttctat
      481 caattcgttg ttgtgtggac agggtctttt cttaaaatta cgggaacaag tgtgggcagc
      541 ttttttattt tcaggaaagg agataaaatc gaaaaacctg ctatatgatc tgaaaaaagt
      601 gaatgacaca gttttattgg aacgtgaact ggaaaacaaa tacgaccgtt tcgctatcaa
      661 aatgttgaaa gatggacggt ttctcggtta cattgctgct tacgaaaata ttgtaatgac
      721 catgctgatg gatcagggtg tacaacttgg agcatcggta agctccgtaa ccgaaaacat
      781 aaatgagacg agatatcttg acaaagtttt ttccgttcag ttattcacca aattaccagt
      841 tccttataac tatttagaaa taaataatct gcgaacaaaa cgggcggatg acgctgtagc
      901 tatttacagg aaaggaacat taataaataa ggagaaacta taaaatttct ccttaaaaaa
      961 gcaattcaca acattcttct catacatcat ataatctatg agatttgtaa tgaaacttga
     1021 atatgcaaat tggttttttg cataagtgtg aggattggtg cacacacaag taaatattgt
     1081 catatctact ttcaagttta tttggcaaag atacacatta ttttcgtaat tatgagaaga
     1141 atgaaaatat atttatatct ttgcattgat aaaatattca aaaacattaa attaacatga
     1201 aaacaatttt aggattagat gtgggtacca acagcatcgg ttgggcactt atcgaacatg
     1261 gccctgagaa cagaaaggga agaattatcg gaatgggaag tcgaattatt tccatgggcg
     1321 cggagttgag taattttgag agaggacagg cccaaaccaa aaacgccaac cggcgcatcg
     1381 ccagaggggc aagaaaactc aataaaagat ataagcaaag acgcaataaa cttatttacg
     1441 cgcttaaaga gttgggaatg ctgcccaacc agattcaatt agtaaaggat ttctttgatc
     1501 cgaacaagat cgataaaatc agtatcaaac ccatcaaaaa gagacaacca caactaacgg
     1561 cttttgattt gttggagcta agggtgaaag ccctttcaga aaaaattgaa ctaaaagatt
     1621 tgggtcggat tatctataaa tataatcaac tcaggggata tgcaggcgga aatgcggagc
     1681 cggacaaaga agacaataac gatgacgcag aatatcagga aaagaaaaac gaaaactata
     1741 tcgcttttgc aagagtcttg caattaggca ttcccgaaga ggtaaccata aaaaacagaa
     1801 aattcaacaa acgaaaaata tcgattgaaa cggatgatgg gattcttgac ggcgaaacct
     1861 atctggattc gttaaaagag aatgattccc tggaattgca agtgaatatc aggcgttcca
     1921 aatcaggaga atccattacc gtaaaattgc ccgataaaac caactggcgc aagaaaatgg
     1981 aaaacctgga gaaagagttg aaagaaaaat ccaaagaatt gggccgggag gtttatattt
     2041 cggaacactt gttcgatatt gtaaaggaaa atccaagagc aaaaatccgg aacaacgtta
     2101 tcttacgttc gcgttatgaa agtgaatttg acgcaatttg ggattcccaa tataaagtaa
     2161 atgaggattt taaaaggtta gtggataata aaaagttatt gacaaaaatt ttatgtttca
     2221 tttttccggg aacgtcaaag actcaagagc aattccgtgc tgaaggatta gaaaaaggtt
     2281 taaaatatgt cttgaaaaat cagatcatat tttttcaacg tgaactcaaa gatcaatcac
     2341 acctaatttc tgactgccgt tttgaaccca aggagaaggt ggcgcccaag agtcatccaa
     2401 tatttcagga atataaaatt tgggaacaaa tcaacaagtt actcataaat accaaaacag
     2461 aagtaggcgt aaaccgtaaa ggagaacaaa aatatacata catagaccgt ccgattcccg
     2521 cttcgttgaa agtaaaacta ttcgatgacc ttcaaaataa aaaggaaata tcctatggtg
     2581 ttatctacaa tgaattgaaa aaaaaatatg agttgcgcga aaaagttgat tttttaaatg
     2641 gattgaacgt caaggcgaaa ctaaaaggca atgagactag gatcatgctt caaaagtcct
     2701 tgggtaatta cctttgggag gctttgaatc ttgatgccat tgagcgacaa acggagcttt
     2761 ggaatattct gtatcatggg aaaggaaacg aatacgacat ccatagcgaa agaacatccc
     2821 gggtgttgag atttttaaaa gaatacggtc ctggaattga taacgtagaa gatacggcta
     2881 ttcggatttc taagataaaa ttcagccgaa gctattcgaa tctaagtttg aaagcgattg
     2941 aaaaactatt accgctggta agggctggaa aatactttga caacgcattt tcaaatgaaa
     3001 ttcattcaag aatagtcaaa ctcttaaatg aaaatgtgga agatcctttt gaaaaagccg
     3061 cacaggaatt cttagaaaag aacagcatgg ttttagaaga aggtggtatc gtaaacgcta
     3121 tggccgtaat tttagtgtat gggaagcata ccgctaaaga atttaccgaa gatgaacttg
     3181 taaagactcc aaccgggata tctttcatta aaaatggttc gcttcgaaat cctttagcag
     3241 agcaaattat caacgaaact ttggcaatgg tcaaagatat ttggaggaaa tatggaggca
     3301 aacccgatga gatcagggta gaattggcgc gtgaactaaa aagcagcgct gaagaaagag
     3361 ccaaaattta ctccgtcaat caaaaaaatc agaaagcaaa tgaagcagta agagaaaaac
     3421 ttatcgagtt gaaagaagag ctttcgattg ccaatatcga gaaatacaaa ctttggcttt
     3481 ctcaggaaaa tttacaggaa gagtatatta aacattattc cgatccgtct aaatccgagg
     3541 tagaaaaatt aaagctctgg gaagaacaag gacatgtttc gccatacacc gggcagccga
     3601 ttccgttaag tcatctgttt aataaagctc gatacgatat tgaccacatc attcccaaat
     3661 cccgttactt tgacgattcc tttacaaaca aagtaatctg tgaaaaagcc gtaaacaccg
     3721 ataagggcaa ccggacagcc atggaatatt ttgaaacggg atcaatgacc caaaagattt
     3781 tcagcaaaga tgattttatc gaccatgcca ataaactctt cacaggcaga aagcgaaaaa
     3841 atcttttagc gacaaaagtt cccgaagatc caattcaacg gcaaataaaa gacacccaat
     3901 atattgccgt aaaagtaagg gaagaactga acaaaattgt gggcaacgag aacgtagaag
     3961 ccacttcggg aggcgttacc gattatttga gaaatcattg ggggctgacc gataaattta
     4021 aatcaatgct gaaagagcga tacgacaaca tacaaccaaa attatcggaa attgcctaca
     4081 acgagtatgt aaaaaacatt caaacaaaac aaaaggaata tgcggaaaag aatattccgt
     4141 ttaatgatat agtattgaat aacgaactgt ttgttgaaaa attcaataag gaatttttca
     4201 aaaagaaaaa caataaactc gtaataaacg gttggagcaa gagaatagac caccggcacc
     4261 acgcgttaga cgcgctaatt attgcttgca cgcagccgcc acatataaag cggctgaacg
     4321 atttgaacaa agtgttggaa gattggttaa aaaaaaataa agagacgttt ttgcctgatt
     4381 ttaacggctc taaagcagaa ttgctggatg aagtgttaaa cctggacgag aacaagcgta
     4441 aaagtatctt caaggaactg gataaattca ccgacattca aattccctgg caaggattcg
     4501 tggaagaagc agaagaagga ttaaaacaaa ttatcgtctc gcacaaaccc aaagacaaat
     4561 tactgttgca gtacgatgaa aaaggaaacc ggcagataaa attgcgtggg caactacacg
     4621 aaggaacgct ctatggcaaa acaaacggaa gggaaagcta ccgtatccct ctgttaaaac
     4681 tggcaggaaa gaattttgca accgaaaaaa taatcgaaaa aatcgtcgat ccgtttctaa
     4741 aaaagcaaat ccgggaacat ctaaatcaat ataaagggaa aaaagatgaa gctttttcgg
     4801 ccgaaggcat attagacttg aataacaaat tagcggagcg caggggtaaa aacgataatc
     4861 cggcacctca cacgccagtt tcgaccataa aaatatacta taaagaccct gacaagaata
     4921 aaaatgcaaa cgatgaagaa attatcacac ttcaaaaatt agaccgaagc aaggctttta
     4981 ataattcctt atatgtgaag accggcgaca attatctttt cgccgttatg gaaaaagatt
     5041 acttcaacaa gaaaacgaga cagacagaaa agaaaagagt tttcgatttg ataacttttt
     5101 tcgatgcagc caacttattg aaaggagagt ttaacaaatc ttccgataaa aacactttcg
     5161 acaaagacct tttattcaaa aaatattttg aggaaaaaaa t
//
LOCUS       DAOSDR010000232         5200 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrimonas sp. isolate AMR_MDS_1826
            NODE_5663_length_5200_cov_4.424684, whole genome shotgun sequence.
ACCESSION   DAOSDR010000232 DAOSDR010000000
VERSION     DAOSDR010000232.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863355
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrimonas sp. (wastewater metagenome)
  ORGANISM  Petrimonas sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Dysgonomonadaceae; Petrimonas.
REFERENCE   1  (bases 1 to 5200)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5200)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:10:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,720
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 35
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5200
                     /organism="Petrimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5663_length_5200_cov_4.424684"
                     /isolate="AMR_MDS_1826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2023866"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..557
                     /locus_tag="PLI30_09365"
     CDS             <1..557
                     /locus_tag="PLI30_09365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007558184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="C-terminal helicase domain-containing protein"
                     /protein_id="HOI79800.1"
                     /translation="GKKQKVKEIAKTLRKMGLSADAMHSDLEQTERNSVMHEFRNERV
                     DILVATDIVSRGIDIDDISLIINFDVPYDAEDYVHRIGRTARAGDSGMAITFVSPDEQ
                     LRFSRIEKFLQKNIYKIPVPPELGDVPEYNPEKRRKEGRKHSDKPQRGIGKKRTQEST
                     EKKAQKKTSKKTQSRPDKNNQTGS"
     gene            complement(574..1134)
                     /locus_tag="PLI30_09370"
     CDS             complement(574..1134)
                     /locus_tag="PLI30_09370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006842394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HOI79801.1"
                     /translation="MLQEIVISGFGGQGVLSMGKILAFSGIMEDKEVSWFPAYGPEQR
                     GGTANVTVIISDKPISSPVVNQYDVAIVLNQPSLDKFEEKIKPGGALIYDDYGIRHKA
                     RRKDINVYRIKAMDESMKMNNTKVFNMIVLGGLLKISPMVQLANVMEGLKKTLPERHH
                     KLLPMNEEAIMKGMDIIELEQEADCL"
     gene            complement(1145..1906)
                     /locus_tag="PLI30_09375"
     CDS             complement(1145..1906)
                     /locus_tag="PLI30_09375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006842395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HOI79802.1"
                     /translation="MDINSIVSPENLVYAKPEIMNDNHMHYCPGCSHGVVHKTIAEVI
                     KELDLQEKTIGIAPVGCAVFAYKYLDIDWQEAAHGRAPALATAAKRLNPDKMVFTYQG
                     DGDLAAIGTAETIHAANRGENIAIIFINNGIYGMTGGQMAPTTLSKMVTSTSPDGRDV
                     QTMGSPLKILNLLALLDGVCLATRVSVHTAAAVKKAKRMIKLAFENAMAGKGTSIVEV
                     VSTCSSGWKMTPAEANDWMAENMFPVFPIGDLKNI"
     gene            complement(2087..3169)
                     /locus_tag="PLI30_09380"
     CDS             complement(2087..3169)
                     /locus_tag="PLI30_09380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013815154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate dehydrogenase subunit
                     VorB"
                     /protein_id="HOI79803.1"
                     /translation="MTEDVTLMKGNEAVAYAAIRYGVDGYFGYPITPQSEIIETLMEA
                     APWKTTGMVVLQAESEVAAINMVYGGAACGKYSMTSSSSPGISLKQEGISYLAGAELP
                     CLIVNVQRGGPGLGTIQPSQSDYFQTVKGGGHGDYRLITLAPNSVQEMADFVSLGFDL
                     AFKYRNPAMILTDGVIGQMMEKVKLPEYRRRRTEQEIREQCPWATLGKTKDRGPNIIT
                     SLELDAALMEKNNERFQAKYRVIEQEEVRYQETACEDADYLLVAFGSAARICLKTIDM
                     ARKEGIKIGLLRPITLWPFPSNILNTYADKVKGMLTVELNAGQMVEDVRLAVNGKVPV
                     EHYGRLGGIVPTPDGVLDALKEKIVK"
     gene            complement(3230..3463)
                     /locus_tag="PLI30_09385"
     CDS             complement(3230..3463)
                     /locus_tag="PLI30_09385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005635283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HOI79804.1"
                     /translation="MAKVQGYVVVNTQRCKGCNLCVVSCPTDVLELQPREVNDRGYHY
                     VYMKNPEECIGCFNCGTVCPEGCLTVYRKRLDK"
     gene            complement(3704..4225)
                     /locus_tag="PLI30_09390"
     CDS             complement(3704..4225)
                     /locus_tag="PLI30_09390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008158604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase family protein"
                     /protein_id="HOI79805.1"
                     /translation="MSAINYDLTKIKSFIFDVDGVLSCQTVTLAEDGQPMRSTNVRDG
                     YAIHHAIRSGLDVAVITGGRSEIVRHRLESLGVRHIYLKSYDKTEQLKEYMDKTGFDP
                     EEIIYVGDDIIDYLVMKKVGLPVAPADACPEIKDISIYVSPVKGGEGVARDVIEQVMK
                     VQGKWMVNDAFFW"
     gene            complement(4275..4808)
                     /locus_tag="PLI30_09395"
     CDS             complement(4275..4808)
                     /locus_tag="PLI30_09395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005647698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HOI79806.1"
                     /translation="MQDFNTLLINRRSYRKYADGPLAGDAVQLILEAALLSPTSKNKH
                     SWEFVVVEDREMLAKLSRCKAQSAAFIADAALAVVVLGNPLETDAWIEDASIAAINMQ
                     LQAEELGIGSCWVQVRNRDFSETITSGEYINGILDVPMPLEVLCVISFGKKEKPRSPN
                     NGDQLLWEKVHLGKYTF"
BASE COUNT         1438 a         1240 c         1054 g         1468 t
ORIGIN      
        1 caggaaagaa acaaaaagtc aaggaaattg ccaaaacgct tcgtaaaatg ggactgtctg
       61 ctgacgccat gcattctgat ctggaacaga cagaacgcaa ttcagtcatg cacgaattcc
      121 gcaacgaaag agtggatatt ctggttgcta ccgacatcgt ttcacggggt atcgatatcg
      181 atgacatatc acttatcata aactttgatg taccctacga tgcagaggat tatgttcatc
      241 gcatcggacg tacggcacgc gcaggagatt cgggaatggc cataacgttt gtctcgcccg
      301 acgaacaact tcgtttttca aggatcgaga agtttctaca aaaaaacatc tacaaaatac
      361 ccgttccccc ggagttgggt gacgttcccg aatacaatcc cgaaaaacgg cgaaaagagg
      421 gcagaaaaca ttctgataaa cctcaaagag gcatagggaa aaaacgcacc caggaatcaa
      481 cggagaaaaa ggcacaaaaa aagacttcta aaaaaacaca aagccggcct gacaaaaata
      541 atcaaaccgg ctcctgattt cgatattaca ggattacagg caatctgctt cctgctccaa
      601 ctcaatgata tccattcctt tcataatggc ttcttcattc attggcaaaa gcttatgatg
      661 acgttcaggc aacgttttct ttaacccttc cataacgttt gccaactgaa ccatgggtga
      721 tatcttaagt aatccgccca atacaatcat attgaacacc tttgtgttgt tcattttcat
      781 ggattcgtcc atagccttaa tccggtaaac gttgatatcc ttacgcctgg ctttatggcg
      841 aattccgtaa tcgtcgtata taagtgctcc gcccggtttt atcttttctt caaacttatc
      901 cagcgaaggc tgattcaaca cgatggccac gtcgtactgg ttaacgaccg gtgaactgat
      961 gggtttatca ctgataataa cggttacatt ggccgttcct ccacgttgtt cgggcccgta
     1021 agccgggaac caggacacct ctttgtcttc cataataccg gaaaaagcaa gaattttacc
     1081 catcgataaa actccctggc cgccaaatcc cgaaataaca atttcttgca acatattttt
     1141 caagttaaat attctttaaa tctcctattg gaaacaccgg aaacatattc tcggccatcc
     1201 agtcgttggc ttcggcaggc gtcattttcc atccggagct acaggttgat acaacctcaa
     1261 cgatggaggt tcccttaccg gccatagcat tttcgaaagc cagctttatc atcctcttgg
     1321 ctttcttgac cgctgctgcg gtatgaacgc ttacgcgggt ggccaaacag acaccatcga
     1381 gcaatgccag caaattgagt attttaagtg ggctacccat ggtttgcaca tcacgtccgt
     1441 ccgggctggt ggaggttacc atcttactga gtgttgtcgg cgccatttgt ccgccagtca
     1501 tcccatatat tccgttattg atgaaaataa tagcgatatt ctctccacgg ttggcagcgt
     1561 ggatggtttc tgcagtacct atagctgcca ggtcgccgtc cccctgatag gtaaacacca
     1621 ttttgtcggg attcagccgt tttgctgcag ttgctaaagc gggcgcacgt ccgtgagcgg
     1681 cttcctgcca gtcgatatcc aggtatttgt atgcgaaaac cgcacatcca accggggcaa
     1741 ttccgatcgt cttttcctgt aggtcgagtt ctttaataac ttcggcaatg gttttgtgaa
     1801 caacaccgtg cgaacagccg gggcaatagt gcatatggtt gtcgttcatg atttccggtt
     1861 tggcgtaaac caagttttcg gggctaacta tactgtttat atccataatt ttatcttttt
     1921 gaaaatctgt ataaaaaata acaaacaacg gcgcaagccc ctgtaatcca cgtaagtttc
     1981 atgttttggc cagaaacgaa ataaatgact attgttgcta agaagaaaag caaaaacagc
     2041 atcgaaaata attgttgtaa ttttgggctc acggctgtac gtttttttat tttactattt
     2101 tttcctttaa tgcatccaat actccgtcgg gagtaggaac gattccaccc agacgtccgt
     2161 aatgctcaac gggtactttc ccgtttacgg ccaatcgcac atcttccacc atttgtccgg
     2221 cattgagttc cacagtcagc atacctttca ccttatcggc atatgtattg agtatgttgc
     2281 ttgggaaagg ccacaacgtt atggggcgga gaagcccaat ctttatgccc tccttcctag
     2341 ccatatctat ggttttaaga cagatacggg ctgccgaacc gaaggcaacc agcaaatagt
     2401 cggcatcctc gcatgcagtc tcctgataac gaacctcttc ctgttcaatt accctatatt
     2461 ttgcctgaaa acgctcgttg tttttttcca tgagagctgc atccaactcc agtgaagtta
     2521 tgatattggg gcctctgtct tttgtttttc caagtgttgc ccacggacac tgttcgcgaa
     2581 tttcctgttc ggtacggcga cgccggtact ccggaagttt cactttctcc atcatttgcc
     2641 cgataacacc gtcggtcagt atcatggccg gattacggta tttgaacgcc agatcaaaac
     2701 ccaacgatac aaagtctgcc atttcctgaa cggaattggg agccagcgtg attagcctgt
     2761 agtcaccgtg ccccccgccc tttacggttt gaaaataatc agactgtgaa ggttgaattg
     2821 tgcctaatcc cggtccgcca cgctgtacat ttacgataag acagggcaac tccgcgccgg
     2881 caaggtagga aattccttcc tgcttaaggc tgattcccgg actcgaggaa gaggtcatgg
     2941 aatattttcc gcaagcggca ccaccgtata ccatgttgat ggccgctact tcactttcag
     3001 cttgtaggac aaccatccct gtagttttcc atggagcggc ctccataagt gtttctataa
     3061 tttcggattg tggggtaatg ggataaccga agtatccgtc tacaccgtag cggattgccg
     3121 cataagcaac agcttcgttt cccttcatta atgttacatc ttctgtcata atttaaattc
     3181 agtttttttg atctccttga gtattagact taaaaaacaa aacgttcact tatttatcca
     3241 atcgtttacg ataaacggtt aaacatcctt cgggacaaac ggttccgcag ttaaagcaac
     3301 cgatgcactc ttccgggttt ttcatgtaaa cgtaatgata gccgcgatca ttcacttccc
     3361 gcggttgtaa ttccaacacg tccgtaggac atgacacaac gcaaagattg caacccttgc
     3421 aacgttgcgt attaacaact acataacctt gtacttttgc cattttattt atttgtggtt
     3481 tttgttggtt tttgcaaaat tagtcgatgt ttcaaatgtt ttcaactttt ctgtgttaaa
     3541 tatttccttt aagtatttta atactatcct ggtaaagagc cggattataa ttgaacctca
     3601 tctattcgcg acaaaaaagt ataaatttta tcaaaaaact ggaaacaaga accgaaccaa
     3661 cataaaaaag ttcgaaatat aacttgggta aatatttaat ttttcaccaa aagaacgcgt
     3721 cgttcaccat ccatttgccc tggactttca tcacttgttc aatgacatca cgggcaactc
     3781 cttcaccacc cttgacgggt gaaacgtaaa tggaaatatc cttaatttca ggacaagcat
     3841 ctgcaggagc caccggtaaa cccacttttt tcatgaccaa ataatcgata atgtcgtcgc
     3901 ccacatagat gatctcttcc gggtcgaagc cggttttatc catatattcc ttcaactgtt
     3961 cggttttatc gtacgatttc aggtagatgt gccttacacc gagtgactcc aggcggtggc
     4021 ggacaatttc cgaacgtcct ccggttatca ctgctacatc cagtccagaa cgtatcgcgt
     4081 gatgaatggc ataaccgtcc cgcacgttgg ttgaccgcat cggctggccg tcttcggcaa
     4141 gcgttacggt ttgacaggac aagactccgt ccacatcgaa gataaacgac ttaattttgg
     4201 taagatcgta attaattgcg ctcatgcaaa tttataaatt tttagacatt tagatttata
     4261 gattcagtgc actgtcaaaa cgtatacttt cccagatgca ccttttccca caacaactga
     4321 tcgccattgt tgggagaacg gggtttttct tttttcccga aagaaatgac acagagcact
     4381 tccagcggca tagggacatc gagtatcccg ttaatgtatt ctccggaagt gattgtttca
     4441 gaaaagtcac ggttcctgac ctgtacccag caactgccga tccccagttc ttcggcctgt
     4501 aactgcatat tgatggcagc gatagaggca tcttcaatcc aagcatcggt ttcaagcgga
     4561 tttccaagaa ccacaaccgc cagtgccgca tctgcaataa aagcagcgct ctgagccttg
     4621 cacctggaaa gttttgcaag catctcccgg tcttccacga ccacaaactc ccaggaatgt
     4681 ttgtttttgg aggtgggtga aagcaacgct gcttccagga taagttgaac agcatctccc
     4741 gccaagggtc catccgcata tttccggtaa cttctccgat tgatcagaag tgtgttaaag
     4801 tcttgcatag ttcctatttc tttcgacaca caaaaatagc cataaaaatt aagacaacac
     4861 agccgcaaac aggaaaaatg tttcggttgt aaaaaaagca agaaagctcc tgaaatcaag
     4921 ggtggtgaaa attctcatgc gttaatcatt tttaattaga atcaatctaa acaaaaaaag
     4981 ttatctttgt atccaaaact actttctatg tatcacgttt gccgaacata tataaaaccg
     5041 gggatgaaat tgtttgactt aatcgaggaa aacccttcgc ttttgctggt aatgcagcat
     5101 ttcaatttcg attttcgggt aggcgatttc accatcagcc aattatgcaa agagaaagga
     5161 atcagtgaga aactgtttat atcgattgcc aacctgtaca
//
LOCUS       DAOSDR010000233         5143 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrimonas sp. isolate AMR_MDS_1826
            NODE_5745_length_5143_cov_3.505700, whole genome shotgun sequence.
ACCESSION   DAOSDR010000233 DAOSDR010000000
VERSION     DAOSDR010000233.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863355
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrimonas sp. (wastewater metagenome)
  ORGANISM  Petrimonas sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Dysgonomonadaceae; Petrimonas.
REFERENCE   1  (bases 1 to 5143)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5143)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:10:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,720
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 35
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5143
                     /organism="Petrimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5745_length_5143_cov_3.505700"
                     /isolate="AMR_MDS_1826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2023866"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..828
                     /locus_tag="PLI30_09400"
     CDS             <1..828
                     /locus_tag="PLI30_09400"
                     /inference="COORDINATES: protein motif:HMM:NF016497.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HOI79807.1"
                     /translation="KVMGGVESYKSRDLKNWEGPVQVFTVPDDNWITGAVWAPEVHTY
                     NGKYYLFATLNSNIQWKKEQQGWPKYLFRGTQIFVSDHPDGPFLPYAKVPHTPMDQMA
                     LDGTLWVEEGRPYMIYCHEWVQVIDGTMVLVELSADLSAPAGDPLTLFHASAAGWSTG
                     SSHDNIPVKSYVTDGCFLYRTKTGKLLMIWSSFMNGEYAIGIAESVTGKIHGPWVQQE
                     KPLFTQNGGHGMIFRTFEGRLCMTFHRPNSPRGQERARIFELQDTGDTLQLLQELTL"
     gene            866..3136
                     /locus_tag="PLI30_09405"
     CDS             866..3136
                     /locus_tag="PLI30_09405"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: protein motif:HMM:TIGR01409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HOI79808.1"
                     /translation="MIERRDFLKKVAISGVALTSANGLTSFTSRFPDTPNNSEGQGII
                     EKEKDTLSAVWKFDFDERNAQLILQNLNVTLKGKLRFLSDDTPWMVCCSRDGVANRNT
                     LVDPRGDVQGYWVLNTVGTQLELLFYHRTAQRFAGRFSFEGTIFFSEDAFPCRTKPVV
                     GERVLNLMSGFADSLLNDSLFSPKDDLLLQFTAANFRIQTVVKGEFSFRLSGRIEDAA
                     ETVFVFNMEPGYFQKRYIPYYQPIDRKRCPVVPTGWMSWNTYFDKASAEDNLAEARIG
                     KKYLQPFGCEFWSIESWQGNSDQLPVRDFYNMNLEVNEKQFPKGMKALADEIRLLGFR
                     PGLWMAPFGTGNELFYKEHHSLFLHDKNGKPISSWNGKYTLDPTVREAREHLREIHRI
                     ASREWGYEFFKIDGMSGRNSGYCAHLYERPEIRACFNDPSCLNPFELCVQAFREGMGE
                     DRVFLACQGHTSGPEARYAEASRIGADIVHPNQPVVWSGVVNQASCLVNQVFTHNIVM
                     IADPDTLLVGDLPLEEARTSATIVALPGQMTFFGDKLAGLEERKMKILQQTLPVADVR
                     PGNLYPYFGTMPVWNLRIQHEFIGKYNVIAFFNWEDQRNFISTTPEELGIENQDYTGF
                     EFWEKQPFNYKSIEKQFSLEVPPHAVRLVAIHPIKDVPQWISSDRHITQNGMEVSAME
                     WESKRMLLNGTVRLVGSFPLSMYIKVPRRYTFGDITCKDAKCRVIRNQDVLSITFQGE
                     ESGETDFSIKFKQKIS"
     gene            3173..4033
                     /locus_tag="PLI30_09410"
     CDS             3173..4033
                     /locus_tag="PLI30_09410"
                     /inference="COORDINATES: protein motif:HMM:NF015000.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HOI79809.1"
                     /translation="MIMNADGRVKRMGFSFRVKLAILIILCCFFNSYAQVINVNIHAH
                     RGGMLEYEENTINALKETYERGIRGYEIDIRQTKDGKLVLLHDASLLRSVGIDRPIEY
                     LTLNEIRKLRTKQGNTIPTLEEVLDFFNDKEEVYVEFEMKTNNPMYDEATLKRYCDQL
                     CDKIYAAKPPTSTYVLTSFDKRPLKYLKENRPKTELMLIKSEGLSENLLQEANELGIK
                     RVACRIDGTTRGMVQHAKEEGFTVCLWPGTSVEDFLLGVALGGDHLCTDVPLAISKWV
                     KANVSWIKLK"
     gene            complement(4110..4183)
                     /locus_tag="PLI30_09415"
     tRNA            complement(4110..4183)
                     /locus_tag="PLI30_09415"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4147..4149),aa:Ala,seq:ggc)
     gene            4270..4578
                     /locus_tag="PLI30_09420"
     CDS             4270..4578
                     /locus_tag="PLI30_09420"
                     /inference="COORDINATES: protein motif:HMM:NF025479.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4286 family protein"
                     /protein_id="HOI79810.1"
                     /translation="MIIYNTTFSVPTELQNKFLDFIRNEYIPLSTQNHVMKEPRLTRV
                     FSKDDNGDASYALEFKSDTIEALEEWNKTVGRKLYFLITTRFGQNIQGFATLLQPIDL
                     "
     gene            4575..>5143
                     /gene="ruvC"
                     /locus_tag="PLI30_09425"
     CDS             4575..>5143
                     /gene="ruvC"
                     /locus_tag="PLI30_09425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006844542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction
                     endodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HOI79811.1"
                     /translation="MIQQPPIKERIILGIDPGTQVMGYGILKVLGNKPALEAMGVMQL
                     DKYENHYLRLAKIYSRVVSLIEEYLPDEMAIEAPFYGKNVQSMLKLGRAQGVAMAAAI
                     ARDVPIFEYAPLKIKMSITGNGRAAKEQVAYMLQKILQIPDANMLPQLDATDGLAAAM
                     CHYYQSLGVQSDGLKYKNWKDFAERNQTK"
BASE COUNT         1520 a         1060 c         1208 g         1355 t
ORIGIN      
        1 aaagttatgg gaggagttga gtcttataaa agtagggacc tgaaaaactg ggaaggtccg
       61 gtgcaggtgt ttactgtgcc ggatgacaac tggataaccg gagcggtttg ggctcccgaa
      121 gtgcacactt ataatgggaa atactacctt tttgccacgt taaattcaaa tattcagtgg
      181 aagaaagagc agcaaggatg gcctaaatac cttttccggg gaacacaaat ctttgtttcc
      241 gaccatcctg atgggccttt cctcccttac gcaaaagtac ctcatacacc tatggaccaa
      301 atggcgcttg atggaacatt atgggttgag gagggacgcc cctatatgat ttattgccac
      361 gaatgggtgc aggtgatcga cggtaccatg gtattggtgg aactgtctgc agacctttcc
      421 gctcccgcag gagacccgct gaccttgttt cacgcttcgg cagccggttg gtccacagga
      481 tcctcacacg acaacattcc cgtaaaatcg tacgttaccg acggatgttt tctttaccgc
      541 accaaaacag gtaaattact gatgatatgg tccagcttta tgaacggtga atatgccatt
      601 ggaatagcgg agtcggtaac cggaaaaata cacgggccat gggtacaaca agagaagcca
      661 ctgttcacgc aaaacggcgg acacggcatg atattccgca ctttcgaagg ccgtctatgc
      721 atgacttttc accgtcccaa ctcaccgaga ggccaggaac gtgcccggat attcgaactg
      781 caagataccg gagataccct gcaactgctt caggaattaa cgctttaacg ttatgcagta
      841 aattatttaa ctacatctgc aacccatgat tgaacggaga gattttttaa aaaaagttgc
      901 aattagcggt gtcgcactaa catccgcaaa tggattaaca agttttacct ctcgctttcc
      961 cgacacacca aataattccg aaggacaagg gattatcgag aaagaaaaag atacactttc
     1021 tgctgtgtgg aaatttgatt ttgacgagag aaatgctcaa cttatcctgc aaaatctaaa
     1081 tgtgacccta aagggaaaat tacgttttct gtcggacgat acgccctgga tggtctgctg
     1141 ctcccgggac ggagttgcca accggaatac cttggtggat cctcgaggcg acgtgcaggg
     1201 ttattgggtg ctcaatacag taggcacgca gctagagcta ctgttctacc accggacagc
     1261 acagcgattc gcagggaggt tctcttttga gggtactatt ttcttttcag aagatgcttt
     1321 tccctgtcgg accaaacccg ttgtcggaga gagggtgtta aatctgatga gtgggtttgc
     1381 cgattcgctc ctgaatgatt ccttattctc tccgaaggat gatcttttat tacagttcac
     1441 tgccgcaaat ttcaggatcc aaacggttgt caagggggag ttttcattcc ggttaagcgg
     1501 acgaatcgaa gatgccgctg agacggtttt tgtcttcaac atggagcctg gttactttca
     1561 aaaacgatat atcccctact atcagcccat tgacaggaaa cgctgtcctg tcgtccctac
     1621 cggatggatg tcctggaaca cctatttcga taaagcttcg gcggaagaca acttggccga
     1681 agcccggatt ggaaagaagt accttcaacc tttcggttgc gagttttggt ccatcgagtc
     1741 ttggcaagga aattccgatc aattgccagt gagggacttc tacaacatga accttgaggt
     1801 aaatgaaaaa caatttccca aaggaatgaa agcgttggcc gatgaaatac ggttgctggg
     1861 ttttcgtccc gggttgtgga tggctccttt tggcacggga aatgaattgt tttataagga
     1921 acaccatagc ttgttcttgc acgacaagaa cggaaaaccc atcagctcct ggaacggcaa
     1981 gtacactctg gatcccacgg taagggaagc gagagaacac ctgcgggaaa tacaccggat
     2041 cgcatccagg gaatggggat atgagttttt taagattgat ggtatgtcgg gacgaaattc
     2101 cggttactgc gctcatttat acgaacgacc cgaaatcagg gcgtgtttca acgatccgtc
     2161 ctgcctcaat ccttttgaac tctgcgtaca ggcattccgt gaaggaatgg gtgaggaccg
     2221 ggtatttctg gcctgtcaag gacatacttc gggtccggaa gcgagatatg cagaggcctc
     2281 ccgtatcggg gcagatattg tacatcctaa ccagccggtc gtgtggagtg gcgtggtaaa
     2341 tcaggcaagt tgtctggtta atcaagtatt tactcacaat atcgtaatga tagcagatcc
     2401 cgataccttg cttgtgggtg atcttccttt ggaggaagca cgtacctcgg ccacgattgt
     2461 cgctttaccc ggacaaatga cattttttgg tgataaacta gctggcctag aggaaagaaa
     2521 aatgaaaatt ctacaacaga cattgcccgt agcagatgtt cgtccaggga atctctatcc
     2581 ctattttggg acaatgcctg tgtggaattt gcggatacaa cacgaattta taggcaagta
     2641 taatgtgatc gcttttttta attgggagga tcagagaaac ttcatatcta caacgccgga
     2701 ggagttaggc atcgagaatc aggattacac cggttttgaa ttctgggaaa aacaaccttt
     2761 taattataaa tcgattgaga aacaattttc gttggaagtt cctcctcatg ctgtgagatt
     2821 ggtggccatc catccgataa aagatgttcc tcaatggatc agtagtgatc gtcacattac
     2881 ccaaaatggt atggaggtgt cggctatgga atgggaaagc aagcgaatgc tattgaacgg
     2941 aactgttcgg cttgtcgggt cgtttccttt atccatgtat atcaaagttc ctcggcgata
     3001 tacattcggg gacataacat gtaaagacgc caaatgtcgt gttattcgaa atcaggatgt
     3061 gctttcgatc acttttcaag gagaagagtc tggggaaacg gacttttcca tcaaattcaa
     3121 acagaagatt agttaatata tcttttttaa aaaaaattaa accataaaag tcatgatcat
     3181 gaatgcagat ggccgtgtaa aaagaatggg tttttcattc agagtgaaat tagcgattct
     3241 aataattctg tgttgttttt tcaacagtta tgcacaagta ataaatgtca atatccacgc
     3301 tcatcgtggt ggaatgcttg aatacgaaga aaatacgatc aatgctctga aagaaaccta
     3361 tgagcggggt attcgcggat acgaaattga tatacggcaa acgaaggacg gtaagttagt
     3421 ccttttacat gacgcatctt tgctccggtc ggtcggcatt gatcgtccca ttgaatattt
     3481 aacattaaat gagatcagaa aattaaggac gaaacaggga aatacaattc cgacattgga
     3541 agaggttctt gattttttca acgataaaga ggaggtatat gtggaattcg aaatgaaaac
     3601 aaataacccc atgtatgatg aagccacttt aaagcgctat tgcgaccagc tttgtgataa
     3661 aatatatgca gcgaaaccgc caacatccac atatgtattg acatcctttg acaaaagacc
     3721 actgaagtat ttaaaagaaa atcgtccgaa aacagaatta atgttaataa aatcggaagg
     3781 attgagtgaa aatctgctgc aggaggccaa cgaacttgga attaaaagag tcgcgtgcag
     3841 aattgatgga accacacgcg ggatggttca gcatgcgaaa gaagaaggat ttacggtttg
     3901 tctatggccc ggcacgtctg tagaggattt tttactaggg gttgccttag gaggtgatca
     3961 tttgtgtacg gatgttcctt tggcaatttc gaaatgggta aaagcaaatg tttcttggat
     4021 taagttaaag tgaaatcatt aatttaggca ctcataaacg acacctgcaa gcatcactca
     4081 cataaataaa catttaatcc tgagataagt ggagaatatc ggagtcgaac cgatgacctt
     4141 ttgactgcca gtcaaacgct ctagccagct gagctaatcc cccatgtttt ttgtttcggt
     4201 ttgcaaagat agagttttta tttaaatttg caacggtatt cgaaaagtaa atttacagaa
     4261 aagaacacaa tgattattta taacactact tttagcgttc cgacagaatt acagaacaag
     4321 tttctggatt ttatccgtaa cgagtacatc cccttatcca cccaaaacca tgtcatgaaa
     4381 gagccaaggc tcacacgtgt tttttctaaa gacgacaacg gggatgcctc ctatgccctg
     4441 gagtttaaat ccgatacaat tgaagccctg gaggaatgga acaaaactgt gggaagaaag
     4501 ctctattttt taataacaac aagatttgga caaaacattc agggatttgc aacacttttg
     4561 caacccattg atctatgata caacaaccgc ctataaaaga acgaattatt ctgggtatcg
     4621 acccggggac acaagttatg ggatacggaa ttctcaaggt cctcgggaac aagccggcac
     4681 ttgaagcaat gggtgttatg cagctcgaca aatatgaaaa ccattacctt cgtctggcaa
     4741 aaatttattc acgggttgtc agcttgatcg aagaatattt acccgatgag atggctattg
     4801 aagcaccttt ttacggtaaa aatgtgcaaa gcatgctcaa acttggccgt gcacaaggtg
     4861 ttgccatggc cgcagccata gcacgagacg ttcccatttt tgaatacgct ccgttgaaaa
     4921 taaaaatgtc cattaccgga aatgggcgtg cggcaaaaga acaggtggcc tacatgttgc
     4981 aaaagattct gcagatccct gacgcgaaca tgcttcctca gctggatgcc acggacggtc
     5041 tggctgcagc catgtgccac tattaccagt ctttgggtgt tcaatccgat ggtcttaaat
     5101 acaagaactg gaaagatttt gcagaacgta atcagacaaa aat
//
LOCUS       DAOSDR010000234         5134 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrimonas sp. isolate AMR_MDS_1826
            NODE_5765_length_5134_cov_3.756448, whole genome shotgun sequence.
ACCESSION   DAOSDR010000234 DAOSDR010000000
VERSION     DAOSDR010000234.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863355
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrimonas sp. (wastewater metagenome)
  ORGANISM  Petrimonas sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Dysgonomonadaceae; Petrimonas.
REFERENCE   1  (bases 1 to 5134)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5134)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:10:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,720
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 35
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5134
                     /organism="Petrimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5765_length_5134_cov_3.756448"
                     /isolate="AMR_MDS_1826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2023866"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..433
                     /locus_tag="PLI30_09430"
     CDS             <1..433
                     /locus_tag="PLI30_09430"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="HOI79812.1"
                     /translation="DGRIIMYIRASGGFQRLSYSSDRGQTWSHIEASNIPSPLSPATI
                     ERIPGTGDWLLVWNNNDGSNLEIKDKRTPITIAVSKDEGKTWEKIKNIHADPDGWYCY
                     IAIHFVDNKNILLSYCAGSQSQKTHLAVTENTLLHIKWLYE"
     gene            complement(481..1335)
                     /locus_tag="PLI30_09435"
     CDS             complement(481..1335)
                     /locus_tag="PLI30_09435"
                     /inference="COORDINATES: protein motif:HMM:NF024258.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HOI79813.1"
                     /translation="MKTPIFMTSSKQHFRLLVSFVGMLILSCTHSFAQSDSTVIVQIT
                     DTQFGFFENNTGFEKETELYTKAVRRINELKPDFVVITGDFVNDSKNILQIGEFKRIT
                     SLIHQNVPVYLSPGNHDLGQSPTKNDFNFYFSNYGRGTDRFSFKHKNLAFIGLNSVIV
                     KSNKNKKEEKKQFRWLRNQLRKAKKSGSIVLFTHYPFFLYDFNEKETYSNQSVEVRDR
                     YFQLFERYGVDAIFAGHLHNNAEASHNGISMITTNAVGKPLGNAEPGMRIITITSEGI
                     KSRYISIE"
     gene            complement(1468..1659)
                     /locus_tag="PLI30_09440"
     CDS             complement(1468..1659)
                     /locus_tag="PLI30_09440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI79814.1"
                     /translation="MKKISLLAALLFFSLIAVAQEKSFIVLRDIHYDLIEDHDMDWLK
                     TKPDDVRQVKEYTQVNSRC"
     gene            2060..4582
                     /locus_tag="PLI30_09445"
     CDS             2060..4582
                     /locus_tag="PLI30_09445"
                     /inference="COORDINATES: protein motif:HMM:NF025851.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 95 protein"
                     /protein_id="HOI79815.1"
                     /translation="MNTKSLILPLLFSYVAVFSQSGKIEPLKATSRTDNISAAWTDRV
                     LFSGKASKPEGRNVLWYREPAKVWEEALPLGNGRLGAMVFGGVADERIQLNESSLWDG
                     YSLDPNNPEGARALPEVQRLIFENKNNEAVQLAEKKMMGIPKGVRPYQSLGELWFDTP
                     HERADNYLRSLDLTTAIATIKYTFEGKEYVRENFVSAADGVMVVRFTSINGGKINASF
                     TLRRQQDAECKKHSSESNTLLLKGRLPIKDTKGNTRGISFAAQVKAITDGGTIGFNGN
                     IMSVAEANSLTLYVAGATDYPGLDNLGQTPLPGNPEQTCLEAINKASAKPYEMLRSAH
                     IADHQAYFNRVALDLGPESALPTDEQLKEAKRTGQPNANLVETYFQFGRYLLIASSRP
                     GTMPANLQGLWAWQMNPPWNADYHTNINLQMNYWPAEISNLAEMHLPLFDLCEALVPF
                     GRRSAKVIYGARGWVVHHLTDAWGFSAPADGPQGVWPMGAAWLAQHPWEHYCHTLDRS
                     FLAKRGYPLMKGAAEFILDFLVTAPEGTAFAGKLVTNPSYSPENAFILPNGERAVFTY
                     GATMDLQIIHDLLTNCIEASKILGVDKNFRSRCTQALKQLAPTRISKETGRIMEWAED
                     YKEVEPHHRHTSHLFALHPGNQISVTGTPDLAEAARKTLIARGDDGTGWSLAWKINMW
                     NRLHDGNHAYKLLSVLLGSKTLPNLFDTHPPFQIDGNFGATAAIAEMLIQSHVRTPDG
                     GFELHLLPSLPSAIPEGSVKGLRARGGFEVDIDWKAGKLNHVSVRSDKGEKLHLRYGG
                     KILTLETQKGQQFFLDEELKVKKRKRDSDQLLPMTNETSISF"
     gene            complement(4912..4983)
                     /locus_tag="PLI30_09450"
     tRNA            complement(4912..4983)
                     /locus_tag="PLI30_09450"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4948..4950),aa:Arg,seq:cct)
BASE COUNT         1546 a         1139 c         1066 g         1383 t
ORIGIN      
        1 ggacggtaga ataataatgt atattcgcgc aagtggaggg tttcagcggt tgtcttattc
       61 atcggacagg gggcaaacct ggagccacat tgaagccagt aatataccgt ctcccttatc
      121 accagcgact atcgaaagga ttcctggtac tggcgactgg ttgctggtat ggaacaacaa
      181 cgatggctcg aatcttgaaa tcaaggataa aaggacaccg ataacgattg ccgtttccaa
      241 agatgaagga aagacatggg agaaaatcaa aaatattcat gctgatccag acggatggta
      301 ttgctatata gcaattcact ttgtagataa taaaaatatt ttactgagtt attgtgctgg
      361 aagccaatct cagaaaacac acttggctgt cacagaaaat acgttattgc atataaaatg
      421 gttgtatgaa taatttttat aacggagctt ggaaatagca tcattaattt cagtagagac
      481 ctattcaata gaaatataac gactttttat accttcggaa gttattgtga ttattctcat
      541 ccccggctcc gcattaccca aaggttttcc aacggcattg gtggtaatca ttgaaatgcc
      601 gttgtgcgat gcttcggcat tattatgcaa atgtccggca aaaatagcat ccacaccata
      661 cctttcgaat aattgaaagt atctatccct cacctctacg ctttgatttg aataagtttc
      721 tttttcatta aagtcgtaaa gaaagaaggg ataatgtgta aataagacaa tactgcccga
      781 tttttttgct ttgcgcagtt ggtttctcaa ccaccggaat tgttttttct cctctttctt
      841 gtttttattc gacttaacaa tgaccgagtt aagaccaata aaagccaaat ttttatgctt
      901 aaatgaaaaa cggtcggttc ccctgccgta gttagaaaaa tagaaattaa agtcattctt
      961 tgtgggtgat tgccccaaat cgtgattacc cggacttagg taaaccggaa cgttctgatg
     1021 gatgagagac gtaattctct taaattctcc aatttgcaaa atattttttg agtcgttaac
     1081 aaaatcgccg gtaataacaa caaaatcagg cttcaattca ttgattcgtc gaactgcttt
     1141 tgtgtaaagt tcagtctctt tttcaaaacc tgtgttattc tcaaaaaatc caaactgtgt
     1201 gtcggtaatt tgcacgatca ccgtggagtc tgactgtgca aaagagtggg tacaagacaa
     1261 gatcagcatt ccgacaaagc tgaccagtag acgaaaatgt tgttttgagg aagtcataaa
     1321 aatgggagtt ttcattaatt aacgcttatt caacaattat ttgagatgat cggatataca
     1381 aatcctttca taaggtgccg aacttattgc cggataatat tcgaaaaata tttcatcgct
     1441 atcctccagc catatccggt atgaacttta gcagcggctg ttcacttgtg tatactcttt
     1501 tacttgccgt acatcatcag gctttgtttt tagccaatcc atatcgtgat cttcgataag
     1561 gtcgtaatga atatctctca aaacgataaa acttttttcc tgagcaacag caattagaga
     1621 aaagaataac aatgcagcaa gcaaggaaat cttcttcata aaacgaatta tttaatattt
     1681 attaggattc ttctgcattt ataccattat aaacatggca acagttcata ttgttgtaaa
     1741 aaaaagtaaa aactatttgt tacttcactc ctgcctcaca tcccggaaat gcaataattg
     1801 ttggtaagtt caattgaatg cttaccgtat cccaacatgt ttatgtgaaa attcaagtaa
     1861 attcttgaag aaatggacaa agcgaattaa taacttgaga tttattttaa ttcaattttt
     1921 aattatcttt gaaaatcagt ttcaactcta aagacaacta aagcatccgc tttataatta
     1981 cacaaatttt gtactgggct ccatttatta atacccgccc tttatttttg tatttatcaa
     2041 acatgaagaa aactcaaaca tgaatactaa atcattaatt cttcctttac tgttctcgta
     2101 cgttgccgtc ttttctcaat ccgggaaaat tgaacctcta aaagccactt cgcggaccga
     2161 caatatcagc gctgcatgga ccgatcgcgt attgttttca ggtaaagcgt ctaaaccgga
     2221 agggcgcaat gttctctggt accgtgaacc ggccaaagta tgggaagaag ctttaccgtt
     2281 aggcaacgga cgtttaggag cgatggtctt tggaggcgtg gctgatgaaa gaattcaact
     2341 taatgaaagt tctttgtggg acggttactc actcgacccc aataatcccg aaggagcaag
     2401 ggctttacct gaagttcaac ggcttatttt cgagaacaaa aacaacgaag ccgtacaact
     2461 ggccgaaaaa aaaatgatgg gcatcccgaa aggtgtgaga ccctaccagt cattgggcga
     2521 attatggttt gacactcctc atgagcgggc agacaactac cttcgcagcc tcgatttaac
     2581 aacagccatc gccacaataa aatacacgtt tgaaggcaaa gagtatgtcc gcgaaaactt
     2641 cgtgtcggca gcagatgggg ttatggtagt ccgtttcacc tcgataaacg gtggaaaaat
     2701 aaacgcatca tttaccttgc gtcgtcagca ggatgcagaa tgcaaaaagc attcgtcgga
     2761 atcgaacaca ttgcttttaa aaggccggct tcctataaaa gatacaaaag gaaatacacg
     2821 cggaataagt tttgcagcac aggtaaaagc cattaccgat ggaggaacaa tcggcttcaa
     2881 cggcaacatc atgtcggtag ctgaagccaa ctcattgaca ctttatgttg caggcgctac
     2941 cgattatccg gggttagaca atctcgggca gacccccctc ccgggtaatc ccgagcagac
     3001 ctgcctcgaa gcgatcaaca aagcttccgc taaaccctac gagatgttga ggtcggctca
     3061 catagctgac catcaggctt atttcaaccg ggttgccctc gacctcggcc cggaatcggc
     3121 ccttccaacc gacgaacaac taaaggaagc gaagaggaca ggacaaccca acgccaacct
     3181 ggtggaaacc tatttccagt ttggccgtta cctgcttatc gccagttcac ggccgggaac
     3241 catgcctgca aacctgcagg gactgtgggc atggcaaatg aatccaccct ggaatgccga
     3301 ttatcacacc aacataaatc tacagatgaa ttattggcct gctgaaataa gcaatctggc
     3361 agaaatgcac ctccccttat ttgatctttg cgaagccctt gttccatttg gccggcgcag
     3421 cgccaaagta atttacggag ccaggggctg ggtagtacat caccttaccg atgcgtgggg
     3481 attttctgcc ccggccgacg ggccacaagg cgtgtggccg atgggtgccg cctggctggc
     3541 acaacatccc tgggaacatt actgccacac actcgataga agttttcttg caaaacgggg
     3601 ttatccactg atgaagggcg cagcagagtt cattctcgac tttttggtaa ccgctcccga
     3661 aggtacggct ttcgccggaa aactggtaac aaacccttcc tattctcctg aaaacgcttt
     3721 tatattgccc aacggtgaac gtgcagtttt tacctatggc gccaccatgg atctgcaaat
     3781 cattcatgat ttgcttacaa attgcattga agcaagtaaa atactgggtg tagataaaaa
     3841 tttccgcagc cggtgtacgc aagcattgaa acagctggcc ccaacacgta tcagtaaaga
     3901 aaccggacgt ataatggaat gggcggaaga ctataaagaa gtagaaccgc atcaccgcca
     3961 tacatcacac ttatttgcat tgcatcccgg gaaccagatt tcagtcaccg gaacgcccga
     4021 cctggccgag gccgcacgca aaacacttat tgcccgcggc gacgacggga ccggctggag
     4081 cctcgcctgg aaaataaaca tgtggaaccg gcttcatgac ggaaatcatg cttacaagct
     4141 attgtcggta ttgttgggtt ccaaaaccct cccgaacctg ttcgacacac atcctccgtt
     4201 ccaaatcgat gggaatttcg gcgccaccgc cgccattgca gagatgttga ttcaaagcca
     4261 tgtccgcacc ccagacggag gattcgaatt gcaccttctc ccatcgctcc catcggcaat
     4321 acctgaaggc tctgtaaaag ggctgcgggc acgcggcggt tttgaggtgg atatcgactg
     4381 gaaagccggt aaacttaacc acgtcagcgt tcgctccgat aaaggggaaa agttgcactt
     4441 acgctacggg ggaaaaatcc tgacacttga aacccaaaaa ggccagcagt ttttcctgga
     4501 tgaggagtta aaagtaaaga agagaaaacg ggattctgat caattattgc caatgacaaa
     4561 cgaaacatca atcagctttt gaaatttccg tcaatcgtaa aaatgaaagg tcctgtcgtc
     4621 gctcatggca acaaatattt ttccgaaaaa agattctccg aaacttaggc agaaacatcg
     4681 tgacatcgat gattaaatat cgctgcggga gttcttctga cttttgttca ggttggtcca
     4741 ctgttcgtag gtaagccgac gttgccttat gccttcttta gccttatcga aattacaact
     4801 gcctcattta ttgattggtt agtgttacgg tgggaaaatg aggatacagc ataataaaat
     4861 cctccccctt ataatcaaca aaaataaagg ggaggattgt tttaatcttt gtagcccggc
     4921 cgggaatcga acccggatct aaagtttagg aaacttctat tctatccatt gaactaccgc
     4981 gccaaataca ctgcaaaagt aatgtttttt ccgatattct caaacatccg gacatatttg
     5041 ctcaaatcaa ttggcccccc aaacaatttt tcatttaaac tgtttagagt gtaaacaatt
     5101 tatttcaacc cataattttt cattcatgag ccat
//
LOCUS       DAOSDR010000235         5129 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrimonas sp. isolate AMR_MDS_1826
            NODE_5773_length_5129_cov_4.442452, whole genome shotgun sequence.
ACCESSION   DAOSDR010000235 DAOSDR010000000
VERSION     DAOSDR010000235.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863355
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrimonas sp. (wastewater metagenome)
  ORGANISM  Petrimonas sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Dysgonomonadaceae; Petrimonas.
REFERENCE   1  (bases 1 to 5129)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5129)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:10:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,720
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 35
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5129
                     /organism="Petrimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5773_length_5129_cov_4.442452"
                     /isolate="AMR_MDS_1826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2023866"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..290
                     /locus_tag="PLI30_09455"
     CDS             <1..290
                     /locus_tag="PLI30_09455"
                     /inference="COORDINATES: protein motif:HMM:NF040138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HOI79816.1"
                     /translation="KLSTTIVVIHTKIMKPTSNFTFSFDDADNKEVLFNNTSLMSEKY
                     QWNFGDGSAVSDDRSPSHVYSSKGTYTVTLTAIGITGNEDKSVKTEVITIE"
     gene            461..1906
                     /locus_tag="PLI30_09460"
     CDS             461..1906
                     /locus_tag="PLI30_09460"
                     /inference="COORDINATES: protein motif:HMM:NF012516.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004650 - polygalacturonase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 28 protein"
                     /protein_id="HOI79817.1"
                     /translation="MKYKYQIRITKFVHPVLFSLFFVSFFGILGCNSTLYNPEKYEHL
                     ISDEPRSEDYEVWINGTKAIVYVARVQDSPWEKERTRLDFGGNYSFTSFDMDKPVEVK
                     IKSTNKTLDHTILRPERVNVKNLKKSSTELSFIIEKPSQIIVEPDGKNGPLLLFANSK
                     DDFVPDLNDPNLIYFGPGIHHPDTALIKVGSNQTLYLAEGAILKAGVRVQGDNVTICG
                     RGIICGNEFVWGKFSRNLILIEKSNNVVVKDVILRGGASWTMPVRHSRNVVVDNVKIV
                     GGRAQNDDGINPCNSQDVLIKNCFIRTDDDCIAAKGTTYLPDTENNVERITVENSILW
                     CDRARIFLLGHESRAEYMRDMVFRNLDIVHFSMTAFLLEPGEDMRLENIVFDNIRIYG
                     EGQAELIRLKPVVNQYMRKKVPGYINNITFKNISVTDKEGPYKIQLIGADKQYTVSNV
                     VFDGVSVLNQKVTKEYGSLEMGDFVSNVEFK"
     gene            2141..>5129
                     /locus_tag="PLI30_09465"
     CDS             2141..>5129
                     /locus_tag="PLI30_09465"
                     /inference="COORDINATES: protein motif:HMM:NF028438.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5107 domain-containing protein"
                     /protein_id="HOI79818.1"
                     /translation="MEIEEEYATYPFSDPDPIPVFGKIYPYFRFDGYTTRSIKKKYKI
                     INLENDYLKIKIFPEIGGKIWSVVDKTCGKEMFYDNKVVKFRDISLRGPWTSGGIEFN
                     YGVIGHDPACSFPVNYLTAQNPDGSVSCWISNFDLITRTRWTVEINLPKDKGWFTTSS
                     FWHNSNSGSQPYYNWVNTGIKATEDLELIYPGTYSIGHGGETIPWPMDEERGKNLSRW
                     TENDFGGSKSYHVAGTYEPYFGAYWTSENFGMMHVTDRDEKVGKKFFTWALSDEGSIW
                     EELLTDTNGQYLEVQSGRLFNQNDAHSSRTPYKQVYFTPYSTDVWTEYWFPFKGTSGV
                     TDASLYGVININEGNGVLDFVISPLVQINDTLKFYDAEGEILSSQPISLSVARTFSTS
                     IRFPVERSVYKITLCNRTIWSKENKTLDRPNSETDNFDYNTAYGKYILGRDYSGMRLY
                     DQAERQIKESLELDPNYIPSLVEMSRLCYRRMNYDSAYFFARKALSFDTYHPDANFEY
                     GRSAKQLGKIFDSLDGFEIAAITTPMRSAAYTEISKIYFITKEYDKSEEYAQKSLINN
                     SLNIEGLQLLYLCYRQQNNLPKSDEIAERIKTIDPFNLLILFESTAPNTHLFLENIHT
                     EMPVQSFLELAIWYYSLGMEKECRAILKAAPENAELKYWLAFLSKDTPEEITLLQQAD
                     AQSPSFVFPFRNECKDVFEWAMSKSNSWKPAYYMALLQGSVNNTEAARTLLNKLGDQP
                     DFPPFYSLRASLSNKDIDKERDLKKAVTFAPTEWRYNQRLTNHYLGKQEYVKAIKTIE
                     PLYAKNKNHFPTVSLYARTLMSNKQYDEAEKLLNAIHILPFEGERGGRVMYREIKMML
                     ATHALAKGKTKRAGEKVLESLQWPRNLGVGKPFDDQIDTRLEDWMLAMIAIKSNKHAD
                     KEHYLKKVTQATQLSNNFSTLLQCLAWSQLGEQQKANDLFAKWSSLQSNPLAVEWAGR
                     FFEENRDKVYPFDVDEMIQLIKVI"
BASE COUNT         1696 a          937 c         1047 g         1449 t
ORIGIN      
        1 ctaagctaag tacgacgatt gttgtcatcc acacaaaaat aatgaaacca acttcaaatt
       61 ttacattttc ctttgatgat gctgacaata aagaagtttt gtttaacaat acttctctaa
      121 tgtcagaaaa atatcagtgg aattttggag atggttcagc agtatcagat gatcgatcac
      181 catcccatgt gtattcctct aagggaactt atacagtcac gctgacagct atagggatta
      241 ctggcaatga ggacaaatct gtcaaaactg aggttattac tatcgagtga tttaaattta
      301 gcaagcaaga gcatctgtaa cagtaaatat tgtctgtttc aggtgctcca gcttctgaaa
      361 attttccctt atatcaagat catgagctct tacaattaca agtaatcatc cataaacggc
      421 ttctttggtt atgaaaagga agtttaaaaa tcttaaaaat atgaaataca aataccaaat
      481 acgtattaca aaatttgttc atcctgtatt attttctctg ttttttgttt ctttttttgg
      541 aatcctcggc tgcaatagta cgttgtacaa tcccgaaaaa tatgaacacc tcatttcaga
      601 tgaaccccgt tcagaagatt atgaagtatg gatcaatgga actaaagcca ttgtatatgt
      661 tgcgagggtt caggattctc cgtgggaaaa agaaagaaca cggcttgatt ttggaggaaa
      721 ttattctttt acctcttttg acatggacaa accggttgaa gtgaagataa aatcaacaaa
      781 caaaacactt gatcatacga ttttacgtcc ggagagagtc aatgtaaaga atttaaagaa
      841 gagtagtacc gagttatctt ttatcattga aaaaccgtcc caaataattg ttgagccgga
      901 tggtaaaaat ggcccattac tcctttttgc aaattcaaaa gatgattttg taccggattt
      961 gaatgaccct aatttgatat attttgggcc gggtatacat catcctgata cagcattaat
     1021 caaagtgggt agcaaccaaa ctctttatct cgcggaaggt gccatattaa aagctggggt
     1081 cagagttcaa ggagataatg taaccatttg tggaagaggt attatttgtg gaaatgagtt
     1141 tgtatggggg aaattttcac gtaatttgat tttaatagaa aaaagtaaca acgtggttgt
     1201 aaaggatgtt atcttaagag gaggtgcttc atggaccatg ccagtaagac attccaggaa
     1261 tgtcgttgtt gataatgtga aaattgttgg aggaagagca caaaatgatg atggcatcaa
     1321 tccctgtaat tcacaagacg tattgataaa gaactgtttt atccgaacgg acgatgattg
     1381 cattgcagca aaaggtacca catatttacc tgatacagaa aataacgttg aacggattac
     1441 cgttgaaaat tcaatcctgt ggtgtgaccg ggcaaggatt tttcttttag gtcacgaaag
     1501 tcgtgccgaa tatatgagag atatggtatt tagaaacctg gatatcgttc attttagtat
     1561 gactgctttt ttattggaac caggagaaga tatgaggtta gaaaatattg tttttgataa
     1621 tattcggatt tacggtgagg gtcaagccga attgatcaga ttaaaacctg ttgtcaacca
     1681 atacatgaga aaaaaagttc ccggatacat taataacatt acattcaaaa acatttccgt
     1741 taccgataag gaagggccct ataagataca attaattgga gctgacaagc aatatacagt
     1801 aagtaatgtt gtttttgatg gggtttcagt actgaatcaa aaggtcacta aggagtatgg
     1861 tagtttggaa atgggagatt ttgtttccaa cgtcgaattt aagtgataat agacaggata
     1921 aaaagaagtc acatcatata gccggatctg tttgagattt taacagtcat ttgtgcaggt
     1981 tgtctgtaca ggagttcagc ctatgaataa gtcaataata tatatctatt caacaatgaa
     2041 tactatacag ccatactggc gttccttaaa aaaattaatt gcattaacct tctgtttact
     2101 ttgtatttta ggtttgaaag cacaaaacac gccacaaata atggaaatag aagaagaata
     2161 tgctacttat cctttttcgg atcccgaccc tatacctgtt tttggcaaaa tttatccgta
     2221 tttccgtttt gatggatata ccacacgatc tatcaaaaaa aaatacaaga taattaatct
     2281 cgaaaatgac tatttaaaaa tcaagatttt tcctgaaatc ggcggaaaaa tatggtctgt
     2341 agtggataaa acctgtggaa aagagatgtt ttatgacaac aaagtggtca aattcagaga
     2401 catctctctg cgtggaccgt ggactagcgg cggtatagag ttcaattacg gcgtaattgg
     2461 gcatgacccg gcctgctcat ttccagtcaa ttacctcacc gcgcaaaatc cagatggtag
     2521 tgtgagttgt tggatcagta atttcgattt gatcactcgt acccgttgga ccgtggagat
     2581 taatctgcct aaagataagg gatggtttac cacttcctcc ttttggcaca acagtaattc
     2641 gggaagtcaa ccctattaca actgggttaa tacaggaatt aaagcgactg aagatttgga
     2701 attgatctat cccgggactt attctattgg tcacggaggt gaaactatcc cgtggccaat
     2761 ggatgaagaa cgcggtaaga acctgtcgcg atggactgag aacgatttcg gcggcagcaa
     2821 atcgtatcat gtagccggca cctatgaacc atatttcgga gcatactgga ccagtgaaaa
     2881 tttcgggatg atgcatgtta ctgatcgcga cgaaaaagtg ggaaaaaaat tctttacctg
     2941 ggcactatcc gatgaaggca gtatttggga agaattattg accgacacca acggacagta
     3001 tcttgaggta caaagcggta gattgttcaa tcaaaacgat gctcacagca gccgcactcc
     3061 atacaaacag gtttatttta caccttacag caccgatgtt tggacagaat actggttccc
     3121 tttcaaaggt acaagtggcg ttaccgatgc aagtctttac ggggtaatca atatcaacga
     3181 agggaatggc gtattggatt ttgtaatttc ccctttggta cagatcaatg acacactcaa
     3241 attttatgat gcagaaggag aaattctttc gtcacaacca atatctcttt ctgtagcgag
     3301 gacatttagt accagtatac gttttcctgt tgagagaagc gtatataaaa tcactctttg
     3361 caaccgtaca atttggagta aagaaaataa aacattggac aggcctaact cagaaacaga
     3421 taatttcgat tataatacag cctatggcaa atacatatta ggaagagatt attcgggaat
     3481 gagactttat gaccaggcag aaagacaaat caaggaatcg ctagaattgg atcccaacta
     3541 cattccgtca ttggtcgaaa tgtcgcggct ttgctaccga cggatgaatt acgattcggc
     3601 atattttttt gcgcgtaaag ctttgtcttt cgatacatac cacccggatg ctaatttcga
     3661 atacggacgt tctgccaaac agttaggaaa aatatttgat tccctggacg gatttgaaat
     3721 tgcagcaatt accactccta tgcgcagcgc ggcttatacg gaaatcagta aaatatattt
     3781 tattacaaaa gagtatgaca agtctgaaga atacgcacaa aaaagcctaa tcaataatag
     3841 tcttaatatt gaagggttac agttattata cctttgttac agacagcaaa ataatttacc
     3901 gaagtccgat gaaattgccg aaagaattaa aacaatcgat cccttcaatc tattaatatt
     3961 gttcgaatca actgcgccca atacacacct gtttttggaa aatatccata ctgagatgcc
     4021 ggttcagtct ttcctcgaat tggccatatg gtattattcg ttggggatgg aaaaagaatg
     4081 ccgagcaatt ttaaaagccg ctccggaaaa tgccgaacta aaatactggt tggcctttct
     4141 tagcaaagac actcccgagg agatcacatt gttacagcaa gccgatgcac aaagcccatc
     4201 gttcgtattc cccttcagga acgaatgtaa agacgtattc gaatgggcaa tgtctaaaag
     4261 caacagttgg aagccagctt attacatggc tttattgcag ggatcggtca ataatactga
     4321 agctgcacgt actttactta acaaactagg cgatcaaccc gattttccac ctttctattc
     4381 gttacgtgca tccctttcca acaaggatat agataaagaa cgcgacctta aaaaagcagt
     4441 tacgttcgca ccaaccgaat ggagatacaa ccaacggcta accaaccatt acctcgggaa
     4501 acaggaatac gtaaaggcga tcaaaaccat tgaaccgctc tatgctaaaa ataagaatca
     4561 ttttcctaca gtatcgttgt atgcacgcac attgatgtcc aacaaacagt atgacgaagc
     4621 cgaaaaatta ttgaatgcca ttcatatttt acctttcgaa ggtgaaagag gagggcgtgt
     4681 catgtaccgc gaaataaaaa tgatgctggc tacccacgca ttggctaaag gaaaaacaaa
     4741 gagagcagga gaaaaagtat tggaatcgtt acagtggccc cgtaacctag gagtgggcaa
     4801 acctttcgac gaccagatag atacccgtct tgaagactgg atgctagcta tgatagcaat
     4861 aaaatcgaat aagcacgccg ataaggaaca ttatttaaaa aaagtaactc aagcaacaca
     4921 actgagtaat aatttttcta cgctgttgca gtgcctcgca tggtcacaac ttggcgaaca
     4981 acaaaaagca aatgatctgt ttgctaaatg gtcttcactt caaagcaatc ctcttgctgt
     5041 ggaatgggct ggccgtttct ttgaagaaaa tcgtgacaag gtgtatccct tcgatgttga
     5101 tgagatgatc cagctgataa aagtcatct
//
LOCUS       DAOSDR010000236         5105 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrimonas sp. isolate AMR_MDS_1826
            NODE_5810_length_5105_cov_4.104158, whole genome shotgun sequence.
ACCESSION   DAOSDR010000236 DAOSDR010000000
VERSION     DAOSDR010000236.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863355
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrimonas sp. (wastewater metagenome)
  ORGANISM  Petrimonas sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Dysgonomonadaceae; Petrimonas.
REFERENCE   1  (bases 1 to 5105)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5105)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:10:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,720
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 35
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5105
                     /organism="Petrimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5810_length_5105_cov_4.104158"
                     /isolate="AMR_MDS_1826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2023866"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            285..806
                     /locus_tag="PLI30_09470"
     CDS             285..806
                     /locus_tag="PLI30_09470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008861388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin"
                     /protein_id="HOI79819.1"
                     /translation="MVSKNLEKAINEQVNAEFWSAYLYLSMSAHFANEGLMGFANWFK
                     VQFQEEQDHALKFMDYLIAKGNKVELKPIEKVDTSWESILKAFEDTLAHEKVVTGLIN
                     NLVGLARSENDYATENMLQWFVNEQVEEEETAQGMIDSLKLIGTNGFGIYTMDKELAQ
                     RSYTPLNTATTGA"
     assembly_gap    948..957
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <958..1274
                     /locus_tag="PLI30_09475"
     CDS             <958..1274
                     /locus_tag="PLI30_09475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI79820.1"
                     /translation="NLKMERKYKKLFKCMVLDAIGMASAALPVIDVVWAPIAASISYR
                     MFGEKRGKYTSVITFIEEILPVTDVIPSFTIFWFLFDFLGVGKEAKTANPPVDTDFVE
                     IN"
     gene            1290..2195
                     /locus_tag="PLI30_09480"
     CDS             1290..2195
                     /locus_tag="PLI30_09480"
                     /inference="COORDINATES: protein motif:HMM:NF014066.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraB/GumN family protein"
                     /protein_id="HOI79821.1"
                     /translation="MNLRKLTGCLILFFTLTACANTTGETNALLWKISGNGLEFPSYL
                     FGTHHLVPLSFLDSIIGLEEAFEATEQTVGELDMSKMSEMQIKIMGAAIMPNEYDYRT
                     LISEADFQLLDKTLKELTGIGLDQLGRMKPAMLSNLISVSLYQKYYPALSGSTSIDQH
                     FQEEAARRSRPIRSLETAEEQIRLLLGTQTIERQAELLTCMVNHPGILKEQMDRLQTA
                     YMAQDIDALNALYEEELPGDPCPGTPEEKDAMNKARNERWLKLLPDIVREKPSFIAVG
                     CLHLVGKDGLIVGLQKMGYRVEPVK"
     gene            complement(2203..3615)
                     /gene="rlmD"
                     /locus_tag="PLI30_09485"
     CDS             complement(2203..3615)
                     /gene="rlmD"
                     /locus_tag="PLI30_09485"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008673884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HOI79822.1"
                     /translation="MAKKRKQLPLLEKVPVTGVAAEGKAIARVDGMTVFVPYAVPGDV
                     ADIQITKKKSNYAEGKVTRFESFSENRADPFCEHFGTCGGCKWQILPYGEQLKYKQKQ
                     VEDNLTRIGKVELPEISPILGAPETTFYRNKLEFTFSDKRWLTEKEISSESEPRQMDA
                     LGFHIPGMFDKVLDINKCWLQDDLSNQIRNAARDFCLKNKFSFFDLRNQSGLMRTLIL
                     RNTSVGEWMVIVVFYEDDAEKREMLLNFLAGKFPQITSLLYIINRKANDTITDQEVIC
                     WKGREYIVEEMEGLQFKIGPKSFYQTNSRQAYSLYKVAREFAGLSGSERVYDLYTGTG
                     TIANFVARNAGKVIGIEFVEEAIEDARKNSRNNNIENTSFFAGDMRKILTVDFIREHG
                     RPDVIITDPPRAGMHDDVIGAILFAEPQRIVYVSCNPATQARDLNLLDSKYRVARVQP
                     VDMFPQTHHVENVVLLERRT"
     gene            complement(3669..>5105)
                     /locus_tag="PLI30_09490"
     CDS             complement(3669..>5105)
                     /locus_tag="PLI30_09490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011202546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4982 domain-containing protein"
                     /protein_id="HOI79823.1"
                     /translation="QDMGVNAIRTSHNMPTPEYVRIADEMGMMLMVETFDEWAIPKVK
                     NGYNRYFNELAEKDLTNVLHHYRNNPSVVMWCIGNEVEEQSNKHGSRVARFLQDICHR
                     EDPTRPVTNGMDRPDHVLSNNMGAVMEVPGFNYRPFRYQEAYDRLPQQVILGSETAST
                     LSSRGVYKFPVERKSMAQYDDHQASSYDVEHCGWSNLPEDDFIQHEDLPYTIGEFIWT
                     GIDYLGEPTPYYSAWPSHSSLFGAVDLAGIPKDRFYLYRSHWNKEEETLHILPHWNWE
                     GREGEVTPVFVYTSYPSAELFINGKSQGKRTKELSVPLEGSYTTEAQKSFERQKRYRL
                     MWMDTRYEPGTVKVVAYDNNGKAVAEKEVRTAGKPYRVVMQADRNVINADGEDISFVN
                     VKLVDKAGNFCPVEDREISFTVKGAGVFKAVANGNATSLESFQLPRMKLFSGQLTALV
                     QAGEEPGTIVLEAKAKGVQSGRIEILVK"
BASE COUNT         1362 a         1285 c         1125 g         1323 t
ORIGIN      
        1 gattcatcag ataaaaatta aaatgagttt ggatatttta tctatttcca ataaaaacca
       61 ggatacaaag atacgaatat ttctcataat gcgaagtggg caggtaaaaa tatactttca
      121 aatcaaaaca actgataata tgacacttga cctcttttac ctttttgttt caggttattg
      181 aaaacccggt cgagtggcac aaaaagcaaa accaaacgta aacattttca tgagaaaaac
      241 gtttaacagg gtaggattaa cgaattatta aaaaaacaaa cactatggta agtaaaaatt
      301 tagaaaaagc tatcaacgag caagttaatg cagagttctg gtcagcctat ctgtatctgt
      361 ccatgtcggc tcatttcgcc aatgaaggat tgatgggatt tgctaactgg tttaaagtac
      421 agtttcagga agaacaagac cacgccctca aattcatgga ttacctgatt gccaaaggca
      481 acaaggtgga gttgaaaccg attgagaaag tagatacctc atgggaatct atattaaaag
      541 ctttcgaaga caccctggca cacgaaaaag tggtgaccgg actgattaac aatctggtag
      601 gcttagccag gagcgaaaac gactatgcga ccgaaaacat gcttcaatgg tttgtaaacg
      661 agcaggttga agaagaagaa accgcgcagg gaatgatcga cagcctgaaa ctgatcggca
      721 ccaacggctt cggaatctat acaatggaca aagaactggc acaacgcagc tatactccgc
      781 ttaacaccgc tacaactggt gcatagtttt ttcaaccaga cacactcagg agccttcttc
      841 gccgaaaggc tccttttttg tttggttaac tttttagcga agagagtctc tgaataaact
      901 ttttaggtta aacttcatct aacatcctgt tctttatcgg actttagnnn nnnnnnnaaa
      961 acttgaaaat ggaaagaaaa tacaaaaagc tcttcaaatg catggtgctc gacgctatcg
     1021 gtatggcgtc agcagcccta cccgtcatcg atgttgtgtg ggcacccatc gcggcatcca
     1081 tcagctacag gatgttcggg gagaaaaggg gaaaatacac atccgttatc acctttattg
     1141 aagaaatact acccgttacc gatgtaattc catcgtttac tattttttgg tttttattcg
     1201 attttctggg tgtcgggaaa gaagccaaaa cggccaatcc ccctgtggat acagattttg
     1261 tcgaaatcaa ctgataccgt tataatttta tgaatttacg aaaactaacc ggctgcctga
     1321 tccttttctt taccctgaca gcctgcgcga acacaacagg ggagacaaac gcgctattgt
     1381 ggaaaatctc gggaaacggc cttgaattcc cgtcctacct gtttggaaca caccacttgg
     1441 tcccgttgtc gtttttagac agcatcatcg gcctggaaga agcgttcgaa gcgactgaac
     1501 aaacagtggg tgagctcgat atgagcaaaa tgagtgagat gcaaataaag atcatgggtg
     1561 ctgccattat gcccaacgag tacgactacc gaacacttat aagcgaagcg gattttcagt
     1621 tgctcgacaa aacactgaaa gagcttaccg gtatcggact ggatcagctt ggcagaatga
     1681 aaccggccat gctgagcaat ttgatttccg tctctttata tcagaaatat taccctgctc
     1741 tatcagggag cacaagcatc gaccagcact tccaggaaga agcggccagg cgttcccgtc
     1801 ccatacgctc tttggaaacg gccgaagaac aaattcgcct attgttgggc acgcaaacca
     1861 tcgaaaggca ggccgaacta cttacttgca tggtgaatca tcccgggata ctgaaagagc
     1921 aaatggacag gttgcaaacg gcgtacatgg ctcaggacat tgacgcgctg aacgctttgt
     1981 acgaagagga gttgcccgga gatccctgcc ccggcacccc ggaagaaaaa gacgcgatga
     2041 acaaggcccg gaacgagagg tggttaaaac tccttccgga tattgtgcgg gaaaaaccat
     2101 cgttcatcgc tgtcggatgc cttcacctgg tgggtaaaga cggactgatc gtgggactgc
     2161 agaagatggg ataccgggtt gagccggtga aataggcaaa acctaggtcc gtctctctag
     2221 caagaccaca ttctccacgt gatgggtttg cggaaacata tccaccggct gcacgcgggc
     2281 gacccggtat ttactgtcca gcaggttgag gtcacgcgcc tgggtggcag gattgcaact
     2341 tacataaaca atccgctgcg gttcggcaaa aagaatggcc ccgatcacat cgtcatgcat
     2401 cccggctcgg ggaggatccg tgataatcac atcgggacgg ccatgctcac gaataaaatc
     2461 taccgtcagg atttttctca tatctccggc aaaaaacgac gtgttttcga tattattgtt
     2521 gcgtgaattc tttcgcgcat cctcgatagc ctcttccaca aattcaatcc cgataacttt
     2581 tccagcattt cgcgcaacaa aattggcaat ggttcccgtt ccggtataca gatcatacac
     2641 ccgctcactg cccgacagcc cggcaaactc ccgcgcaact ttatacaggc tgtaagcctg
     2701 acgactattg gtctggtaaa atgatttcgg gcctatttta aattgcaggc cttccatttc
     2761 ttccacaata tactcacgcc ctttccaaca gatcacttcc tggtcggtaa tggtgtcatt
     2821 ggccttccgg ttaatgatgt agagcagcga agtgatttgc ggaaacttgc cggctagaaa
     2881 attcaggagc atctcccttt tttcagcatc atcttcgtaa aacactacaa taaccatcca
     2941 ctctccaaca gaggtgttgc gcaggataag cgtacgcatc agcccggact ggttgcggag
     3001 gtcgaaaaac gagaatttgt ttttcaggca aaaatcccgg gcagcattcc gtatctggtt
     3061 agacaggtcg tcctgcaacc agcatttgtt tatatcaagc actttatcga acataccggg
     3121 gatatggaac cccaacgcat ccatctgcct gggctcactc tcggagctaa tctccttctc
     3181 cgtcagccac cgtttgtctg aaaatgtgaa ttcaagtttg ttccggtaaa aagtggtttc
     3241 cggagcaccc agaatgggtg aaatttccgg caattctacc ttgccgatgc gcgtcaggtt
     3301 atcctcaacc tgtttttgtt tgtatttcaa ctgttcccca taaggcagga tctgccactt
     3361 acagcccccg catgttccaa aatgttcgca aaaagggtcc gccctgtttt cggaaaacga
     3421 ttcaaaccgg gtcactttgc cttcagcgta attacttttc tttttggtga tctgtatatc
     3481 ggccacatca ccgggaacgg catacgggac aaaaaccgtc attccgtcca cccgggcaat
     3541 tgctttgcct tctgccgcta caccggtaac aggtaccttt tccaataaag gaagttgctt
     3601 tctctttttt gccattgcct atttttttga aggtgcaaag gtatgcaata ttccattcag
     3661 cccgtcatct attttaccag aatctcaatc cttccgcttt gtactccttt tgcttttgcc
     3721 tccagcacga tcgttcccgg ttcttctccg gcctgcacca acgcagtaag ctgtccgctg
     3781 aaaagcttca ttctggggag ttggaacgat tccagactgg ttgcatttcc attggcaaca
     3841 gctttaaata caccggcacc ttttaccgta aaagaaattt ccctgtcttc aaccgggcag
     3901 aaatttccag ctttatccac aagcttcacg ttgacgaacg aaatgtcctc gccgtcagcg
     3961 ttaatcacgt ttcgatcggc ctgcataaca acgcgatacg gttttccagc ggtacgcact
     4021 tctttctcgg caaccgcttt tccgttgttg tcgtacgcca ccaccttcac cgttcccggt
     4081 tcataacggg tatccatcca catcaaccgg tagcgcttct gccgttcgaa actcttttgc
     4141 gcttctgtcg tataacttcc ttcaagcggg acagacagtt ccttagtacg ttttccctgg
     4201 cttttcccgt tgataaacaa ctctgccgac ggataactgg tgtacacaaa aacgggcgtc
     4261 acttcccctt cacgcccttc ccaattccag tggggcagga tgtgcagcgt ttcttcctct
     4321 ttattccagt ggctgcgata gagataaaaa cggtccttgg gaattcccgc cagatcaacc
     4381 gcgccaaaaa gggagctgtg actgggccac gcgctgtagt acggcgtcgg ttcccccagg
     4441 taatcgatgc ccgtccagat aaactctccg atggtatacg gcaaatcttc gtgttgaata
     4501 aaatcatcct caggaagatt cgaccaaccg caatgctcta catcgtatga agaggcctga
     4561 tgatcatcgt actgcgccat ggatttacgc tccaccggaa acttgtatac ccctcgggaa
     4621 ctaagcgtag atgccgtttc actgcctaag attacctgtt gcggtaagcg gtcgtacgcc
     4681 tcctgataac gaaacggacg ataattgaat cctggaacct ccatcacggc ccccatgttg
     4741 ttggaaagca catggtcggg acggtccatt ccgttggtta ccggacgagt cgggtcttcg
     4801 cggtggcaaa tatcttgcag gaaacgtgcc acccttgaac cgtgcttgtt actttgctcc
     4861 tctacttcat tgcctatgca ccacatcacc acactgggat tgttccgata atgatgcagc
     4921 acgttcgtca ggtccttctc cgccaactcg ttaaaatagc ggttgtagcc gtttttcact
     4981 ttcggaatgg cccattcgtc aaacgtttcc accatcagca tcattcccat ttcatcggca
     5041 atccgaacat attcaggtgt aggcatattg tgtgaagtgc ggatagcgtt tacacccata
     5101 tcctg
//
LOCUS       DAOSDR010000237         5074 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrimonas sp. isolate AMR_MDS_1826
            NODE_5864_length_5074_cov_3.725443, whole genome shotgun sequence.
ACCESSION   DAOSDR010000237 DAOSDR010000000
VERSION     DAOSDR010000237.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863355
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrimonas sp. (wastewater metagenome)
  ORGANISM  Petrimonas sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Dysgonomonadaceae; Petrimonas.
REFERENCE   1  (bases 1 to 5074)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5074)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:10:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,720
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 35
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5074
                     /organism="Petrimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5864_length_5074_cov_3.725443"
                     /isolate="AMR_MDS_1826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2023866"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            245..1960
                     /locus_tag="PLI30_09495"
     CDS             245..1960
                     /locus_tag="PLI30_09495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019539489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005283 - amino acid:sodium symporter
                     activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:alanine symporter family protein"
                     /protein_id="HOI79824.1"
                     /translation="MQQINDFLTTLHNYIGGNYWFIFLLLGTGTFFTVYLRFPQIRYF
                     RHAIKIVKGKYDKSTDKGDTSHFQALATALSGTVGTGNIAGVALAVHLGGPAAIFWML
                     LTAFLGMCTKFVEVTLSHKYRDFDEKGMVAGGPMYYMKKRLNISIKNGYRIKTGYWMG
                     GFFAVATVLSSFGSGSLPQINSISNSVFATFGIKHVITGAIMAFFLALIIIGGIKRIA
                     KVTERLVPFMAIFYFVGALAVILFNYQNIIPSFASIFLDLFTGTAATGGFLGAGFAFA
                     FNQGVNRGLFSNESGQGSAPIAHAAAKAHEPVSEGMVAILEPFIDTIIICFLTGLVLL
                     SSGVWKEKLPNQFQKTDIEVLTAHYSENKPSDVSRLENHLNQTARLPLFSGKLDIKDG
                     QLQENVSIIHARSLASEVVVLESGQPFTGRIDVVNGKVTTTPYNVTFRGNSLIHSAPL
                     TTAAFSKSFFGDFGKYIVSIGLLLFAFSTSIAWSYYGDRAVTYLFGANYVLIYRIVFV
                     IAFFFASFTDTTIIWNVSLLTVAFMAIPNLFGLLVLHREVKSTIKDYWKGFRQEYPDE
                     KTPEK"
     gene            2099..2629
                     /locus_tag="PLI30_09500"
     CDS             2099..2629
                     /locus_tag="PLI30_09500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002559111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma-70 factor"
                     /protein_id="HOI79825.1"
                     /translation="MFNEYYDRFIRFAWGYVKEKQVAEDFVSEAFTTYWENKEDLLPD
                     TKPHAYILSIIKNKCINYLQHLQVRQRAEKEINAHAEWLLSTRINTLQACDPDFIFSD
                     EIQKIVESTLNKLPQKTRQVFILNRYQGLSYKDIANQMDLSTKAIEFHMSKALAQLRF
                     SLKDFIHLLLFLFYFQ"
     gene            2700..3620
                     /locus_tag="PLI30_09505"
     CDS             2700..3620
                     /locus_tag="PLI30_09505"
                     /inference="COORDINATES: protein motif:HMM:NF016648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR family protein"
                     /protein_id="HOI79826.1"
                     /translation="MNKELLYRFFEGNASVNEEKQIKQWLDASEANHRVFFQERKVYD
                     AILLTTRGTSLKQKRKVLLSPWATGAAAAIALLLIVSGLYLLIGKDSTGPYNTLFVPP
                     GQRINLILADNSNVWLNSNTEFRYPSSFSKKNRTVYLNGEAYFDVSKNEKKPFIVKTG
                     QGDVRVTGTSFNVEAYSRFNSFETSLFEGGVEIYKNEVKLAALKPNEKAVLSNNGLLV
                     VSKIEDTDEYLWRNGLIAFNNKQLEEILLSLEKYFDIKIQIDSKKLPQHTYTGKFRQS
                     DGVDYALRVLQRSIRFTYERNEETGTIYIR"
     gene            3774..>5074
                     /locus_tag="PLI30_09510"
     CDS             3774..>5074
                     /locus_tag="PLI30_09510"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HOI79827.1"
                     /translation="MKKNYSLGNHLTEKVPIKHFLLIMRTTFILLFTCIFCSMAEMSY
                     TQNARVTINKRNVTLKEVLNEIEKQTDYLFIYSDEVDTNEKVSVKSKQQAVSNVLHAL
                     LKNKDVNYALEGNHILLSKRAEGVEEGLADAQQQKRKITGTVVDNAGIPVIGANILEV
                     GTTNGNITDIDGNFSLTVANDAVITITYIGYLNQTIQTAGKSNFNIILMEDTQALDEV
                     VVIGYGTAKKKDLTGAISAVDGSIVSSQSVSTVSRALEGAVAGLQVSSVDGQPGMDMG
                     IRVRGVGSANLNSSVALVVIDGVPAQSDNPLSTINPNDIQSITVLKDAASTALYGSRG
                     ANGVVLVTTKRGKSGKTNISFSAKAGFNSVGPFDLGKIDNAKDFYEFAWKSIYNSYRY
                     GVNGTGQPQNWTTNVNNPNYSHEQAAEFASQHLFNDINSEST"
BASE COUNT         1548 a         1096 c         1053 g         1377 t
ORIGIN      
        1 caaaggtctt cattttgcaa gcaaggaaaa atacaagtac gattactctt gcaaagataa
       61 aaactttggt ttataatgat ccaaaatttc tttatatttt cttaagtctt ttggccggtc
      121 ttttaatgaa atatgtatat ttgcgaaaca tttggcgtca ctcactaaac cggttgccgt
      181 gaaagacggt tagcagggta acattatact cgcgaaagat ggctattaaa aaccacctta
      241 tcatatgcaa caaataaacg acttcttgac taccctgcat aactacatcg gaggaaacta
      301 ctggtttatt tttctcctgt tgggaacggg tacctttttt actgtttacc tccggtttcc
      361 ccagatcagg tatttccgcc atgccattaa aatagtaaaa ggcaagtacg ataaatcaac
      421 agacaaagga gacacctccc attttcaggc actggcgaca gcattatccg gaaccgtggg
      481 aacgggaaac attgcaggag tagctctcgc cgttcacctg ggagggcccg ccgccatctt
      541 ctggatgctg cttacggctt ttcttgggat gtgtacgaaa ttcgtggagg taacgctgtc
      601 tcataagtac cgtgatttcg acgaaaaagg catggtagcc ggaggcccga tgtattacat
      661 gaaaaaacgg cttaacattt ccataaaaaa cggataccgg ataaaaaccg gttactggat
      721 gggcggcttt tttgccgtag caactgtcct ttcatccttt gggtcgggaa gtttaccgca
      781 gataaacagc atatccaact ccgttttcgc tactttcggc atcaagcacg tcattacggg
      841 agccatcatg gcttttttcc ttgccctgat cattattggt gggatcaaac ggattgccaa
      901 ggttaccgaa cggttggttc cgtttatggc catcttttac tttgtgggag cattagctgt
      961 tatcttgttt aattaccaga atataatccc ctccttcgcc tctatctttc ttgacctgtt
     1021 cacgggaaca gctgctacgg gaggattctt aggtgcggga tttgcttttg ctttcaacca
     1081 gggagtaaac agggggttgt tttccaacga atccggacaa ggctcggcac ccattgccca
     1141 cgctgctgcc aaagcgcatg aacccgtttc cgaagggatg gtggctattc tggaaccctt
     1201 tatcgacacg atcattatat gtttcttgac gggactggta ttgttgtcat ctggggtgtg
     1261 gaaagagaaa ctgcccaacc agtttcagaa aacggatatt gaagtcttga cagcgcatta
     1321 cagtgaaaat aaaccgtccg atgtctcgcg tctggagaat cacctcaacc agacagccag
     1381 acttcccctt tttagcggaa agctggacat taaagacggt cagttgcagg aaaatgtatc
     1441 catcatccat gctcgttcat tggccagtga ggtggtggtc ctggaatcag ggcaaccttt
     1501 cacgggccgg atcgatgttg taaatggaaa agtgacaacc actccctata atgtaacgtt
     1561 ccgtgggaac tcacttatcc acagtgcccc tcttacaaca gccgcgttca gcaagagctt
     1621 cttcggcgat tttggaaaat acatcgtttc tatcggcttg cttttgtttg ccttttccac
     1681 gtccattgcc tggtcgtatt acggagacag ggctgtaact tatcttttcg gagccaatta
     1741 tgtactgatt taccgcatag ttttcgtgat cgccttcttt tttgcgtcgt ttaccgatac
     1801 tactatcatc tggaacgtgt cgctactcac ggtagcgttt atggccattc ccaatctttt
     1861 cggcctactg gtgttacaca gggaagtgaa atcaacaata aaagattatt ggaaaggatt
     1921 ccggcaagaa tatcccgatg aaaaaacacc ggaaaagtga cttgtctttg aatttgttgg
     1981 cctgatccta aacaatattt ttatcaaata atttcaatag acagtttttt gtgcctattt
     2041 ttgctttata aattcaggac tgatgaattc tttctcttcc gtacaacatt tcaataattt
     2101 gtttaacgaa tattatgatc gttttattcg tttcgcatgg ggatatgtaa aagagaaaca
     2161 ggttgcagaa gattttgtat cggaagcttt tacaacgtac tgggagaaca aggaagacct
     2221 tttaccggac acaaagcctc atgcttacat tttgtctata attaaaaata aatgcatcaa
     2281 ttatttacaa cacctccagg tgcgtcaacg tgccgagaaa gaaataaacg ctcacgctga
     2341 atggctatta tctacacgaa taaatacctt acaggcctgc gatccggatt tcattttttc
     2401 agacgaaata cagaaaattg tagaatccac ccttaacaaa ttaccccaga aaacacgcca
     2461 agtatttatt ttaaaccgat atcagggctt atcctacaaa gacattgcca accaaatgga
     2521 tttgagcact aaagctatag aatttcatat gtcaaaagcc ttggcccaac ttcgcttttc
     2581 gttaaaagac ttcattcact tgttgctttt tttattttat tttcaataaa aaatacattt
     2641 tccgttaggg taattcactc ctgaactgtt atacatatac aggataaaga aactaacgcg
     2701 tgaacaaaga acttttatac cgtttcttcg aggggaatgc ttcggttaat gaagaaaaac
     2761 agataaagca atggcttgat gcttctgaag ccaatcatcg tgtattcttt caggaacgaa
     2821 aagtctatga tgcaatctta ttaaccacac gagggacctc attaaaacaa aaaaggaagg
     2881 tcttgctgtc gccatgggca acaggagctg cagcggctat agccctgctg ctcatcgtta
     2941 gcggattata cttgctcatc gggaaagatt caaccggacc gtacaacacc ctatttgtac
     3001 cgcccggcca acgtatcaac ctcatccttg ccgataattc taacgtatgg ctcaattcga
     3061 ataccgaatt tcgctatccg tcctcatttt caaagaaaaa ccgaacggta tacctgaacg
     3121 gtgaagccta tttcgatgtt agcaaaaacg aaaaaaaacc gtttatagta aaaaccggcc
     3181 agggggatgt acgtgttacc ggcacatcgt ttaatgttga agcatactcg cgattcaatt
     3241 ctttcgaaac gagtcttttt gaaggaggtg tggagattta caaaaatgag gttaaactcg
     3301 cggctttaaa accaaacgaa aaagcagttc tgagcaacaa tggattatta gttgtgtcaa
     3361 aaatagaaga taccgacgaa tacctctgga gaaacggcct gatcgcattt aataataaac
     3421 agttagaaga gattctgtta tccctcgaaa aatatttcga tataaaaatt caaatcgatt
     3481 cgaaaaagtt accgcaacac acatacactg gaaaattccg tcaatccgac ggtgttgatt
     3541 atgcattgcg ggtgctgcaa agaagcatcc ggttcactta cgaaagaaac gaagaaacag
     3601 gaacaattta tataagataa aacgtactaa aacacgtgtg cctatgagat aaacgaatta
     3661 cagaccacct ggaaaacaag aatcggcaag cgctgcaacg ctcaccgatt atgcgttttc
     3721 gtcaacacaa ccaagaaata ttcaatgtat taactaaaaa caattacaaa tgtatgaaga
     3781 aaaactattc attgggtaat catttaaccg aaaaagtgcc cattaaacat tttttattga
     3841 ttatgcgaac aacctttatt ttattgttta cctgcatctt ttgctctatg gccgaaatga
     3901 gctacactca aaatgccagg gtgacgatca ataaacgaaa cgtaacactc aaagaagtat
     3961 taaacgagat tgaaaagcaa accgattatc tgttcatcta cagcgacgag gtagatacga
     4021 acgagaaggt atcggtaaaa tccaaacaac aagctgtttc caatgtactc catgcacttt
     4081 taaaaaataa agacgtcaat tatgcgttgg aaggcaatca catcctcctc tccaagagag
     4141 cagaaggagt agaagaagga cttgccgatg cacagcagca gaaaaggaaa attaccggga
     4201 cagtggtgga taatgcaggc atacctgtaa ttggcgcaaa tatcctggag gtaggcacca
     4261 ccaacggaaa cattacggac atagatggca acttttcctt aactgtagcg aacgacgcgg
     4321 taatcaccat tacctatatc ggctacctga atcaaacaat ccaaactgca ggcaaatcca
     4381 atttcaacat catcctgatg gaagacacac aagcgttgga tgaagtggtg gtgattggat
     4441 acggtactgc caaaaagaag gacttaaccg gagcaatctc cgccgttgac ggttcaatcg
     4501 tatcctctca gtcggtatct acggtatccc gcgctcttga aggtgctgta gcaggcttgc
     4561 aggtttcttc tgtagacggc cagcccggta tggatatggg tatacgtgta cgcggtgtgg
     4621 ggtcggcaaa cctaaacagt tctgtagcgc tggttgtgat tgacggagta cccgcgcaaa
     4681 gcgacaaccc actgtcaacc atcaatccga acgatattca aagcatcacg gtattaaaag
     4741 atgcagcatc tacggcacta tacggttccc gtggtgcaaa cggtgtggtg cttgtcacta
     4801 ccaagagggg aaaatcaggt aaaaccaata tctcattttc tgccaaagca ggtttcaatt
     4861 ccgtgggacc gtttgacttg ggaaagatcg ataacgcaaa agatttttat gaatttgcat
     4921 ggaaatcgat ttataactca taccgttatg gggtaaacgg tacaggacaa ccgcaaaact
     4981 ggaccaccaa tgtaaacaac cccaattatt cacatgagca ggccgcagaa tttgccagcc
     5041 agcatctgtt caacgacatt aattcagaat caac
//
LOCUS       DAOSDR010000238         5062 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrimonas sp. isolate AMR_MDS_1826
            NODE_5887_length_5062_cov_3.583184, whole genome shotgun sequence.
ACCESSION   DAOSDR010000238 DAOSDR010000000
VERSION     DAOSDR010000238.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863355
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrimonas sp. (wastewater metagenome)
  ORGANISM  Petrimonas sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Dysgonomonadaceae; Petrimonas.
REFERENCE   1  (bases 1 to 5062)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5062)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:10:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,720
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 35
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5062
                     /organism="Petrimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5887_length_5062_cov_3.583184"
                     /isolate="AMR_MDS_1826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2023866"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..448
                     /locus_tag="PLI30_09515"
     CDS             <1..448
                     /locus_tag="PLI30_09515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019538219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HOI79828.1"
                     /translation="VSGEYKSHAYDHYVMKQGYLSLSGISVVRVRVKGEKGYITVKGA
                     VGEGGITRREWEYEVPLRDAEEMLLLCEDAVIEKTRYLVKVGKHTFEVDEFGGENAGL
                     QIAEVELESEEESYEKPGWLGHEVTGNVRYYNSYLSIHPYREWAEQ"
     gene            complement(481..1500)
                     /locus_tag="PLI30_09520"
     CDS             complement(481..1500)
                     /locus_tag="PLI30_09520"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007210510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HOI79829.1"
                     /translation="MDTINWADLSFGYMKTDFNIRTYFKDGKWSEPQVDTSEFLNIHM
                     AATCLHYGQEAFEGLKAFKGKDGKIRIFRMRENALRLQSSCHGIMMAELPLELFEEMV
                     LLAVKKNERFVPPYESGASLYIRPLLIGTSAQVGVKPASEYTFLIFVTPVGPYFKEGF
                     KPTPMVILREYDRAAPLGTGTFKVGGNYAASLTAGEKAHKMGYSAVLYLDAKEKKYLD
                     ECGPANFFGIKNNTYITPESTSILPSITNKSLMQLAEDMGMKVERRRVPEEELATFEE
                     AGACGTAAVISPILRIDDLVENKTYAFCKDGKAGPISEKLYKKLRAIQYGDEPDTHGW
                     VTIVE"
     gene            1613..3667
                     /gene="dnaG"
                     /locus_tag="PLI30_09525"
     CDS             1613..3667
                     /gene="dnaG"
                     /locus_tag="PLI30_09525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006844570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HOI79830.1"
                     /translation="MIDSQTIERIQDTAQIVDVVSDFVTLRRRGVNFVGLCPFHDDRT
                     PSFYVSPAKNICKCFACGEGGSSVHFIMKHEQLSYYEALKFLAKKYNIEVVEKELTSE
                     EKQAQSDRDSMFILNEFARDYFVKKLHDDPEGKAIGLSYFKERGFRDDIIKKFQLGYS
                     LEQRDAFSSEAQRAGYRSDYLLKTGLSAGGENNSPLIDRFRGRVIFPVHTLSGKVVAF
                     GGRILKKAENTGKYVNSPESEIYSKSRELYGIFFSKSAIVKADKCFLVEGYTDVLSMH
                     QAGIENVVASSGTALTHGQIRMIHRFSENITVLYDGDAAGIKAALRGIDLLLEDGMNV
                     RVVLLPEGEDPDSFARKQSAEEFNRFIEEHEQDFIRFKTHLLLDEVGDDPIKKAGLIT
                     NIVQSIALIPDNIKRSVYIQDTATLLSTREDVVIAAVNKIRIRNYEKKKEQHEKEESR
                     ETAVGTASLPGDTADNGSRKILTARNPYEKPERELIRYIIRYGTLPIFVRYEKEKQKV
                     EDEEIEVEVTIEDGPSVIEVIRFDLMRDKFIFSSEAFFSNTLYRAIFDEACGKVSDES
                     FVCDRYFLTHHDPGISKLATDLISDKYQLSKIHAKSIGESEDEKSSRLRERNSLDKLV
                     IRATTELKNAHVMQRINEVKKNIETADAQQQMELMNELRQLQDLKKVLAKNLGERIIL
                     RY"
     gene            3692..4594
                     /locus_tag="PLI30_09530"
     CDS             3692..4594
                     /locus_tag="PLI30_09530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006801153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOI79831.1"
                     /translation="MMYLETTNVVKQYANHLALNKVSIRVPKGTVFGLLGPNGAGKTT
                     LIRIITRITAPDSGEVLINGRPSKSDDVFNIGYLPEERGLYKKMKVGEQAMYLAQLRG
                     LSKTDAHRELMVWFRRFDIANWYNRKVEELSKGMQQKVQFISTVIHNPDLLIFDEPFS
                     GFDPVNAEMIKNEMLRLKAEGKTIILSTHNMESVEALCDNIALIHRSQVVLQGKVFDI
                     RREHRTNIFRFRLRDSDFSFEHPSFTLVSKEPYHEFTDIRVRKQPEVATNELAKLIFD
                     KFDVVAYDEEIPTMNDVFIETVSS"
     gene            4599..>5062
                     /locus_tag="PLI30_09535"
     CDS             4599..>5062
                     /locus_tag="PLI30_09535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019538223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOI79832.1"
                     /translation="MNTLSLVIQREYLTRVRKRSFIIMTLLMPLLMVALSFVPLWLST
                     LNDGAVKNIAVIDNTGIYAPLLKSTDLYRFQIINDAQQGEYQSKIGKDLFAILQITED
                     LSRNSQAVTILSEKQTPQELRTMVESALDEKVMEQRLDDLSRQGNVNAEAIS"
BASE COUNT         1387 a         1146 c         1292 g         1237 t
ORIGIN      
        1 cgtcagcggt gaatataaat cacatgctta cgaccattat gtcatgaagc agggctatct
       61 ctccctgtcg ggtattagcg tagtgcgggt acgtgtgaag ggagagaaag ggtatatcac
      121 cgttaaaggc gctgtgggag aaggggggat cacccggcgt gagtgggagt atgaggttcc
      181 gctccgcgat gcggaagaga tgctgcttct ctgtgaagat gccgtgattg agaaaacccg
      241 ttacctcgtg aaggtgggaa agcatacctt tgaagtagat gagtttggcg gagaaaatgc
      301 agggctgcaa attgcggagg tggagctgga gagtgaagag gaatcctacg aaaaacccgg
      361 atggttgggt cacgaggtta ccggtaatgt ccgctattac aactcttatc tttccatcca
      421 tccctacagg gaatgggctg agcagtaaca aaaaaaagcg gcaatcgccg ctttttggtt
      481 ttattcaacg atggttaccc acccgtgcgt atccggctcg tcaccgtact ggatagcgcg
      541 aagtttctta tatagttttt ccgagatcgg gccggccttc ccgtctttgc agaatgcata
      601 ggttttattt tccaccaggt cgtcgatgcg caggataggg ctgattactg cggccgttcc
      661 gcacgctccg gcttcttcaa aggtggccag ttcttcttcg ggaacacggc ggcgttccac
      721 tttcattccc atatcttctg ccagttgcat caggcttttg ttggtgatgg aaggcaagat
      781 ggatgttgat tcgggcgtga tataggtgtt gtttttgatg ccgaaaaagt tggcaggccc
      841 gcattcatcc aggtattttt tctctttggc atccaggtaa agaaccgcag aataacccat
      901 tttgtgggct ttttcacctg ctgtgaggct tgcagcatag ttgccgccca ctttaaaggt
      961 cccggttccg agtggcgcag cacggtcgta ttcacgcaag atcaccatgg gtgtaggttt
     1021 aaacccttct ttaaaatagg ggcctacggg ggttacaaag atcaggaaag tgtattccga
     1081 tgctggtttt actcccacct gtgcactggt tccaatcaaa agcgggcgga tatataacga
     1141 tgcgccgctt tcgtaaggcg gcacaaaccg ttcgtttttc tttacggcca gtaaaaccat
     1201 ctcttcaaat agttccaagg gaagctctgc catcatgatc ccgtggcacg atgattgcag
     1261 acgtaacgca ttctcacgca tacggaaaat ccggattttt ccgtcttttc ctttaaaggc
     1321 ttttagtcct tcaaatgctt cctgcccgta atgcagacaa gtggcagcca tgtggatgtt
     1381 taaaaactcg gaggtatcga cttgtggttc actccatttt ccgtccttga aataggtgcg
     1441 gatattgaag tctgttttca tatagccgaa cgataaatcg gcccaattga tcgtatccat
     1501 ttttgttgtg tttaaaataa taaagtcagg ttttataccc tatgcaaaag taacatttat
     1561 tttcaaatta tcccttattg acgattaatt tcactatttt cgtattccca aaatgataga
     1621 ttcgcaaacc atagaacgga ttcaggacac ggctcagatc gttgatgtgg tgtctgattt
     1681 tgtcacgctt cgccggaggg gcgttaactt tgtggggctg tgtccgtttc acgacgaccg
     1741 gaccccgtcg ttttatgtgt cgcccgccaa aaacatctgc aaatgtttcg cttgtggtga
     1801 aggcggttct tctgtgcatt ttatcatgaa gcacgagcag ctttcctatt acgaagcact
     1861 gaaattcctg gcaaaaaaat acaacattga agttgttgaa aaagagctga ccagcgagga
     1921 aaagcaagcc cagagcgatc gcgacagcat gttcatcctg aatgagttcg cgcgtgatta
     1981 ttttgtgaaa aagcttcacg acgacccgga ggggaaagcc atagggttga gttatttcaa
     2041 agagcgcgga tttcgggacg acataatcaa aaaatttcag cttggttaca gcctcgagca
     2101 gcgcgatgcg ttttcctcgg aagcgcaaag ggccggttac agaagcgact atttgctaaa
     2161 aaccggactt tccgccggag gtgaaaataa cagcccgctg atcgatcgat tccggggaag
     2221 ggtcattttt cccgtgcata cgttatcggg gaaagtagtg gcctttggcg gccgtattct
     2281 caaaaaggcg gagaacacag gaaagtacgt aaattcgccg gaaagtgaga tctactccaa
     2341 aagtagggag ctttatggga tctttttctc taaaagtgcc attgttaaag ccgataagtg
     2401 ttttttggtt gaaggttata ccgatgtgtt gtcgatgcac caggcaggta tcgagaacgt
     2461 ggttgcatcg tcgggaacag cgcttacgca cggacagata cggatgattc accgcttttc
     2521 cgaaaacatc acggtcctgt acgacggcga tgcagccggt attaaagctg ccctgcgcgg
     2581 tatcgaccta ctgttggaag acggaatgaa cgtaagggta gtgctgctgc ccgaaggtga
     2641 agatcccgat tcgttcgccc gcaagcagag tgctgaagag ttcaaccggt ttatcgaaga
     2701 gcatgagcag gactttatcc gctttaaaac ccacttgctg ttggatgagg ttggggatga
     2761 tcctatcaaa aaggccgggc ttatcacgaa catcgtccag agtatcgcgc ttattcccga
     2821 caacatcaag cgctccgttt acatacagga tacggcaaca ttactcagca cgcgcgaaga
     2881 tgttgttatt gctgccgtca ataaaatcag gatcaggaac tacgaaaaga agaaagaaca
     2941 gcacgagaag gaggaatccc gggaaacggc cgttggaacg gcatccttac ccggcgatac
     3001 tgcagataac ggaagccgta agatcttaac ggcaagaaac ccttacgaga aaccggaacg
     3061 cgaactgatc cggtacatca tccggtacgg gacgctaccc atttttgtga ggtatgaaaa
     3121 agaaaaacaa aaagtggaag acgaagagat tgaagtggag gtaaccatcg aggacggacc
     3181 gtccgttatc gaagttatcc ggttcgatct catgagggat aaatttattt tttcaagcga
     3241 agcctttttt tccaacacac tataccgggc aatttttgac gaagcctgcg gaaaggtaag
     3301 tgacgagtcg ttcgtttgtg acaggtattt tcttacccat cacgatcccg gaatcagcaa
     3361 actggctacc gatcttatca gcgacaaata ccagttgagc aagatccacg caaaatcaat
     3421 cggagaaagc gaagatgaga aaagctcacg ccttcgcgaa cgaaattcac tggacaagtt
     3481 ggttatccgt gccaccacag agcttaagaa tgctcacgtg atgcagcgga taaacgaagt
     3541 gaaaaaaaac atagaaactg cagatgccca gcaacaaatg gagttgatga acgaacttag
     3601 gcaattgcag gatttgaaga aagtattggc caagaatctg ggagaaagga taatactgag
     3661 gtattaggta tctactgaat aactacagaa tataatgtat ttagaaacca ccaacgttgt
     3721 aaaacagtat gccaaccatt tggcgctgaa caaagtcagc atccgggttc ccaaggggac
     3781 agttttcggg ctgctaggac cgaacggagc ggggaaaacc acccttatcc gcatcatcac
     3841 gcgtatcact gcacccgaca gcggtgaggt gcttatcaac ggacgcccgt cgaagagcga
     3901 tgatgtcttc aatatcggat acctgcccga agagcgtgga ttatacaaaa aaatgaaagt
     3961 aggcgagcag gccatgtacc tggctcaact gcgcggatta tcgaaaaccg acgcacatcg
     4021 cgagctgatg gtttggttca ggcggttcga tatcgcgaat tggtacaacc gcaaggtgga
     4081 agaattatcg aaaggaatgc agcagaaagt gcagtttatt tccaccgtca tccacaaccc
     4141 cgacctgctg atcttcgatg aaccgttcag cggcttcgac ccggtaaacg ccgagatgat
     4201 aaaaaacgaa atgcttcgcc tgaaggccga agggaagacc attattctct ccacgcacaa
     4261 catggagtcg gtggaagcgt tgtgcgacaa tatcgcgctt attcaccggt cgcaggtcgt
     4321 cttgcaggga aaggtcttcg atatccggcg ggaacaccgg accaacattt ttcgttttcg
     4381 ccttcgtgac agcgacttct cgtttgaaca tccctccttc acgctggtct caaaagagcc
     4441 ctaccacgaa tttaccgata tccgggtcag gaaacaaccc gaagtcgcca ctaatgaact
     4501 ggcgaaactt attttcgata aattcgatgt ggtggcatac gacgaggaaa tacctacgat
     4561 gaatgatgtt ttcattgaaa ccgtatcaag ctaaaattat gaatacctta tcattagtta
     4621 tacaacgcga ataccttacc cgggtaagga aaagatcgtt tatcatcatg acgctgctga
     4681 tgccgttgct gatggtggcg ctgtcatttg tgcccctgtg gctctccacc ctgaacgacg
     4741 gtgcggtgaa aaacattgcc gtcatcgaca ataccggcat ctatgcaccg ttattgaaat
     4801 ccaccgacct ctaccggttt caaatcatca acgatgcgca gcaaggcgag taccaatcta
     4861 aaataggaaa agacctgttt gccatcctgc agattaccga agacttgagc aggaatagcc
     4921 aggcggtgac catattatct gaaaagcaga cgccgcagga actgcgtacg atggttgaaa
     4981 gtgcactgga cgaaaaggtt atggaacagc ggctggacga tttgtctcgg cagggcaacg
     5041 taaacgcgga agccatctcg ga
//
LOCUS       DAOSDR010000239         5031 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrimonas sp. isolate AMR_MDS_1826
            NODE_5925_length_5031_cov_4.134445, whole genome shotgun sequence.
ACCESSION   DAOSDR010000239 DAOSDR010000000
VERSION     DAOSDR010000239.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863355
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrimonas sp. (wastewater metagenome)
  ORGANISM  Petrimonas sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Dysgonomonadaceae; Petrimonas.
REFERENCE   1  (bases 1 to 5031)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5031)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:10:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,720
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 35
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5031
                     /organism="Petrimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5925_length_5031_cov_4.134445"
                     /isolate="AMR_MDS_1826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2023866"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..266
                     /locus_tag="PLI30_09540"
     CDS             <1..266
                     /locus_tag="PLI30_09540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008146753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HOI79833.1"
                     /translation="RQSVQFLIFSSKYEEIASHINAELHRGCTVIDGTGWYSKQPQKV
                     LVVLARKTESTPIFRLVKRIDKDAFISQSNVVGVYGKGFDQIK"
     gene            complement(286..1176)
                     /locus_tag="PLI30_09545"
     CDS             complement(286..1176)
                     /locus_tag="PLI30_09545"
                     /inference="COORDINATES: protein motif:HMM:NF014324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004620 - phospholipase activity [Evidence
                     IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase A"
                     /protein_id="HOI79834.1"
                     /translation="MMVTKNLLSATLALFLFIPEINAQLEASRTDSTTWPIKSLTQDR
                     QDLAMTEDEIISYMDELPPFSIYKDNYFITGIPLNRSIDRESADAKFQLSVRHRLTSS
                     RLPFNSFLYLTYTQKSFWDIYRESAPFKDSNYNPGIGLGRYIIVDNRLTGAIFLQLEH
                     ESNGRDSLESRSINWIGLSGKYFFTSNLAASFKITVPFMTGEDNSDLYDYRGTGYFTA
                     DYKTSDNQWWLSGTYSLRKSVKAINLKFTAGYKISEKFNQYIFGELYSGTGDSLLEYK
                     RKDVQLRVGICIKPDFYNVF"
     gene            complement(1189..2616)
                     /locus_tag="PLI30_09550"
     CDS             complement(1189..2616)
                     /locus_tag="PLI30_09550"
                     /inference="COORDINATES: protein motif:HMM:NF024484.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3943 domain-containing protein"
                     /protein_id="HOI79835.1"
                     /translation="MYRSILTYCVLLALPLYGQGRTDSVAEKNKGKSVSINIGLNMVM
                     RGFNRIILKDEYAQINLRSMRTNLKTWPVWDINRFSTNFIGHPYHGSMYFSSARTNGL
                     GFYQSSSVSMAGSLMWEYLMETKPPSQNDLWATTIGGTALGETLFRFSDLVLDNRTTG
                     LERVGRELLAGILAPTRLITRLTTGEAWRTGQSRGNFLHAPSYWLELYFGETATGTTG
                     SNPSMWRSALGIELLYGELFETVAGKPYDWFRLTGEIRAGNNYLHLSQANTLGLMLNK
                     ELLHNNETRVTVGLFQHFNYYDLNGRTNNNTAPVYISEAAAIGPGIIVQKKKDKSTVQ
                     FAVHLSGIILGASTSDHFKLEDRDYNFGSGFSLKLSSSLQFKQRLTISLFSEDYFLFS
                     WKGVDDYEVLKQLTINEIDFLNVQGDKSSTMLNLLGLTMKYSLNKRVHVVLRARSFNR
                     NTLYTYFSSVKHSLNELFFGMSVNV"
     gene            complement(2622..3758)
                     /locus_tag="PLI30_09555"
     CDS             complement(2622..3758)
                     /locus_tag="PLI30_09555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009317279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOI79836.1"
                     /translation="MKTLKFLIEKEFKQMFRNPLIPRLIVLFPTMVLLVFPWAVSFEI
                     KNIRVDVVDHSKSVYSKRLTDKIAASQYFILHDTPPDYNAAMLNMENDETDMILEIPA
                     SFDVDLVKRRESGVGVAVNSVNGTQGLLGSNYVMQIVNDFSSELRGELTQTLPPQTRV
                     SFMRLKKMNIVPQYKYNPALDYKKFMIPGFMVLLLTILCGILPALNIVMEKENGTINQ
                     INVTPISKMNFILAKLIPYWAVGLVVMIISITLSFLIYGLWPGGGVVAVLISAIVFIL
                     SVSGLGIIISNYSETMQQASFLVMFFILILVLLGGMFTPVSSMPAWAQAIAAINPFNY
                     LTTAFRMLYLNGSSLADVSGNLLALGAIAVVLNGWAVFSYKKRG"
     gene            complement(3773..4879)
                     /locus_tag="PLI30_09560"
     CDS             complement(3773..4879)
                     /locus_tag="PLI30_09560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008619170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOI79837.1"
                     /translation="MKQFIAFVKKEFRHIIRDNRTLLIILGMPVVEVLLFGFAVNMEV
                     QNIRVAVYDPTPDAFTTGIAERIRQNSYFNYRGSVQTMDEMEESMRKGELDLAVVFQQ
                     NFQEELVHSGEADVQLLSNSSDPNRGSIAATYATAIIAGFQREQTDLIQIPFQIQTEN
                     RMIYNPLMKSSYMFVPGIMGLVLMIICTIMTSVSIVREKERGTMEVLLASPLKQGTMI
                     FAKTIPYMVISFIDFVIILLLSYFVLDVPVNGSLLLLFFVAILYITLTLSYGLTVSTL
                     VDKQVNAVIFSAITAMIPVLMLSGMIFPVDNMPGALQALSAIVPARWFIEAVRKVMIQ
                     GLGMTIAWKEMLVLAGMTLFLLGVTIMKTKKRLE"
     gene            complement(4879..>5031)
                     /locus_tag="PLI30_09565"
     CDS             complement(4879..>5031)
                     /locus_tag="PLI30_09565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008660546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOI79838.1"
                     /translation="DEAEYCNGVSIMVDGRIDALDTPAELRRRFSADSMDEVFRQLAR
                     KAKRGD"
BASE COUNT         1258 a         1236 c         1177 g         1360 t
ORIGIN      
        1 caaggcagtc ggtgcagttc ctgatcttct cttcaaaata cgaggaaatt gcttcgcaca
       61 tcaacgccga gctccacagg ggatgcaccg ttatcgacgg aacgggatgg tattcgaaac
      121 agccgcaaaa agtactcgtc gtcttggccc ggaaaacgga atcgacacct attttccggt
      181 tggtaaaacg tatcgataaa gatgctttta tctcgcaaag caatgtggtc ggggtatacg
      241 gaaaagggtt cgatcagatc aaatgatttc cacgggatac tctcattaaa acacattgta
      301 aaaatcgggt ttgatacaaa tccctacgcg cagttgcaca tctttccgtt tgtattcgag
      361 caggctatcg ccggttccgc tgtatagttc accgaaaata tattgattga acttctctga
      421 gattttgtag ccggcggtga atttcaggtt tatggctttt acgctcttcc ggaggctata
      481 ggtgcccgag agccaccatt ggttgtccga ggttttgtag tctgcagtga agtatcctgt
      541 tcccctgtaa tcgtataaat cgctgttgtc ttctcccgtc atgaaaggga ctgttatttt
      601 gaaactggcc gcgaggttgg aggtgaaaaa atatttcccg gagaggccta tccagttgat
      661 gcttctcgac tcaagactgt cacggccgtt ggactcatgt tccaactgca gaaatattgc
      721 tcccgtaaga cggttgtcaa cgatgatgta ccttcccagt ccgatccccg ggttgtaatt
      781 actgtctttg aatggggcag actcccggta gatatcccaa aatgattttt gtgtgtaagt
      841 aaggtagaga aaagagttga agggcaacct gcttgaagta agcctgtgcc tgacgctaag
      901 ttgaaatttg gcgtcggcac tctccctgtc gatacttcgg tttaaaggga tgccggtgat
      961 aaaataattg tccttataga tggaaaaggg aggcaattcg tccatatagc tgatgatttc
     1021 atcttctgtc atggccagat cctgcctgtc ttgcgtaagg ctcttaatcg gccatgttgt
     1081 tgaatcggta cggcttgctt ctaactgagc atttatttca ggaataaata aaaacaatgc
     1141 cagcgtggcg gataacaaat ttttcgttac catcatgatt taaattgatc aaacatttac
     1201 gctcattcca aaaaaaagtt catttaagct gtgcttcaca ctggaaaaat acgtatacaa
     1261 ggtgttcctg ttgaaactcc gggccctaag cactacatga acccttttgt tcaggctgta
     1321 tttcatggtt aatcccagta aattcagcat ggtgctgctt ttatcgcctt gtacgttgag
     1381 gaaatcaatt tcatttattg tcaactgttt cagtacttcg tagtcatcca cacctttcca
     1441 cgagaaaaga aaataatcct cagagaataa agagatcgtc agcctctgtt taaactgtaa
     1501 ggaactgctc aatttcaggc taaagccgct cccgaaatta taatccctgt cttcgagctt
     1561 aaaatgatct gaggtgcttg ctcccaggat aatcccgctt agatgaaccg cgaattgtac
     1621 tgtgcttttg tcctttttct tctgaacgat tattcccggg ccaattgccg ctgcttcgga
     1681 gatataaacc ggtgccgtat tattgtttgt tctcccgttt aggtcgtagt agttgaaatg
     1741 ctggaacaaa cctacagtta cccgtgtctc gttgttgtga agcagttcct tgttgagcat
     1801 gagcccgagg gtattggcct gcgacaggtg gaggtagttg ttgcctgcac ggatttctcc
     1861 cgtcagccgg aaccagtcat acggctttcc ggcaacagtt tcaaacagct cgccgtagag
     1921 cagctcaatc cccagagcag atctccacat gctcgggtta ctgccggtag tacctgtcgc
     1981 tgtttctccg aaatacagtt ccagccagta agaaggagcg tggagaaagt ttcctctgga
     2041 ttggcctgtt ctccacgcct cacccgtggt cagccgggtg atgagccggg taggggccag
     2101 gatgccggca agaagctccc tgcccacacg ctccagcccc gttgtccgat tatccaatac
     2161 cagatcggag aaccggaaca gggtctctcc cagggccgtt ccgccgatcg ttgtagccca
     2221 caggtcgttt tgagagggcg gttttgtctc catcaggtat tcccacatca ggctgccggc
     2281 catactgaca gaggaagatt gataaaaccc caacccgttc gtccttgcac tgctgaaata
     2341 cattgacccg tggtagggat gtccgataaa gtttgtggaa aacctgttta tatcccatac
     2401 aggccatgtc tttaaattgg tccgcatcga ccttaaattg atctgggcgt attcatcctt
     2461 taggatgatc cggttgaacc ctctcatcac catgttcaaa cctatgttga tagagacaga
     2521 cttgcccttg tttttctcgg caacgctgtc tgtcctgccc tgtccgtaaa gtggtagggc
     2581 caacaacacg caatatgtca gtatcgatct atacacccac gttatcctct ctttttataa
     2641 ctgaatactg cccatccatt caatactacg gctattgctc ccaatgccag caggttcccg
     2701 gagacatcag ccaggctgct gccgttcagg taaagcatcc ggaaggctgt cgtcaggtag
     2761 ttgaaagggt tgatggccgc gattgcctga gcccatgcgg gcatgcttga gaccggggtg
     2821 aacatgcctc ccagcaggac caggattagg ataaagaaca tcaccaggaa actggcctgt
     2881 tgcatggtct ccgaatagtt ggaaataatg attcccaggc ccgacacgga gaggataaag
     2941 acaatggccg aaataagcac tgcaaccact cctccgccgg gccataggcc gtagatcaga
     3001 aacgacaggg taatggagat aatcatcaca acgaggccga cggcccagta aggtatcagt
     3061 ttggcaagga taaaattcat cttgcttatc ggtgtcacgt tgatctggtt aatggtgccg
     3121 ttctctttct ccattacaat attgagtgcc ggcaaaatgc cacacagaat cgtaagcaac
     3181 agtaccatga agcccgggat cataaatttt ttgtaatcca gtgcagggtt gtacttgtac
     3241 tgcgggacaa tattcatctt cttcaggcgc atgaaggaca cccgtgtttg gggcggcagt
     3301 gtctgcgtca attccccgcg cagctccgac gaaaaatcgt tcacgatttg catgacataa
     3361 ttgctgccca acagcccttg agtcccgttt accgagttca cagcaacccc tacgcccgac
     3421 tctcttcttt tcaccagatc cacatcaaac gaagcgggta tctccaggat catatcggtt
     3481 tcgtcgtttt ccatattcag catcgccgca ttgtaatcgg gtggggtgtc gtgcaaaata
     3541 aagtactgtg aagcagctat tttgtcggtt agtcttttgg aatagacgct tttgctgtga
     3601 tcaaccacat ccacacggat attttttatc tcaaaactta ctgcccacgg gaaaaccagc
     3661 aacaccatgg tgggaaacaa cacgattagc cttggaatca acggattcct gaacatctgt
     3721 ttaaattctt tttctatgag gaattttaat gtcttcattc ggattctatt ttttactcca
     3781 gcctcttttt agtcttcata atggtaactc ccagcaggaa cagcgtcatt cccgctaata
     3841 cgagcatttc tttccaggct atggtcattc ccagtccctg gatcatcact tttctcacgg
     3901 cttcaataaa ccaacgtgct ggtactatgg ccgacagtgc ctgcaatgcc ccgggcatgt
     3961 tatctaccgg gaagatcatc cctgatagca taagcacggg aatcatggcg gtgatagccg
     4021 agaagatgac cgcattgacc tgtttgtcta ccagtgtgga aacggtcaat ccatatgaca
     4081 aagttagggt tatgtagagg atagcaacga aaaacagcaa caggaggctg ccgttgacag
     4141 gcacgtccag cacaaaatag ctcagaagca gaataatgac aaagtcgata aaagagatca
     4201 ccatataggg aatggtcttt gcgaagatca tggttccctg tttcaacggc gatgcgagga
     4261 gtacctccat ggtccctctc tctttttcac ggacgattga caccgaggtc atgatggtgc
     4321 aaatgatcat cagtaccaac cccatgatgc ccggtacaaa catataggat gatttcatca
     4381 gggggttgta gatcatccgg ttctcagtct ggatctgaaa cggtatctgt atcaagtcgg
     4441 tttgttcccg ttgaaatccc gctatgatag ccgtggcata ggttgccgcg atggagcccc
     4501 ggtttgggtc ggaactgttg gaaagcagtt gtacgtcagc ttctcccgag tgaaccaact
     4561 cctcctggaa gttttgctga aagaccactg ccagatcgag ctcacctttc cgcatcgact
     4621 cttccatttc atccatggtt tgaacgcttc cccgataatt gaagtaggag ttctgccgta
     4681 tccgttccgc gatgcccgtg gtgaaagcat cgggagtggg gtcgtagacg gccacacgaa
     4741 tgttctgtac ctccatgttt acggcaaagc cgaacaacag aacttccact acaggcatcc
     4801 ccaggattat cagcaaggtc ctattgtccc ggatgatatg ccggaactct ttttttacaa
     4861 atgctataaa ttgtttcatt agtcccccct ctttgcttta cgtgccaatt gtcgaaatac
     4921 ctcatccatg gagtcggcgc tgaacctgcg gcgtaactcg gccggtgtgt ccaaggcgtc
     4981 gatacggcca tccaccatga tcgatacccc gttgcagtat tccgcttcgt c
//
LOCUS       DAOSDR010000240         5003 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrimonas sp. isolate AMR_MDS_1826
            NODE_5970_length_5003_cov_3.617421, whole genome shotgun sequence.
ACCESSION   DAOSDR010000240 DAOSDR010000000
VERSION     DAOSDR010000240.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863355
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrimonas sp. (wastewater metagenome)
  ORGANISM  Petrimonas sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Dysgonomonadaceae; Petrimonas.
REFERENCE   1  (bases 1 to 5003)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5003)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:10:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,720
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 35
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5003
                     /organism="Petrimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5970_length_5003_cov_3.617421"
                     /isolate="AMR_MDS_1826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2023866"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            33..845
                     /locus_tag="PLI30_09570"
     CDS             33..845
                     /locus_tag="PLI30_09570"
                     /inference="COORDINATES: protein motif:HMM:NF024777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LamG domain-containing protein"
                     /protein_id="HOI79839.1"
                     /translation="MKKRTFIYQLVLITVLGLVISSCFQDLGQNPPFDYPEEPPKPPI
                     SEDGQLFYMTFNDNYKDARSNKEASITGNPGFAGGKVEKAYAGAKDSYLTFKLSDMPA
                     ALGPAVTVGFWYKVNASPDRAGIIVIGPVSEGAPANAQNNRTSGFRIFRENAGGKQRI
                     KANVGNGTADGWLDGGDKADIDPTIFPWKYLALVLTEGKAFFYIDGEEVASSDLSKVS
                     WAGCDIMSIGSGEPRFNEWGHLSDNSLIDELRIYNKALTASQIKEIVNHDTE"
     gene            848..2212
                     /locus_tag="PLI30_09575"
     CDS             848..2212
                     /locus_tag="PLI30_09575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018463075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucoamylase family protein"
                     /protein_id="HOI79840.1"
                     /translation="MIRIFLPLITLLILLSGCSDKKNEQNETPKPETRPLTFASDEAF
                     LDFIQQAHINYMWDGAEKTSGLAPERMHIDGNYPENDADVITTGGSGFGIAGLVVGIE
                     RGFIPRTDGVARLHRIVDYLTKADRFHGIWPHWIHGPTGKVKPFGLKDNGGDLVESAF
                     LMQGLLCVREYFKDGSENEKQLATKIDGLWREMEWDWYLNGKDVLYWHWSPEYGWEMN
                     FPLEGYNECLITYILAASSPTHPISASAYHNGWARGGAIRSSAVAYGLPLILKHNGAE
                     EYGGPLFWAHYSYIGLNPKGLKDQYADYWEVNTNQARINYNYCKQNPKGFQGYGENCW
                     GLTASYSVNGYSAHMPVKNDLGVITPTAALSSFPYTPEESMKALKHFYVELGDSIWGK
                     YGFYDAFSIQDNWYPQRYLAIDQLTIAPMIENHRSGLLWNLFMGAPEIQKGLQNLGFE
                     FLNN"
     gene            2368..4695
                     /gene="bglX"
                     /locus_tag="PLI30_09580"
     CDS             2368..4695
                     /gene="bglX"
                     /locus_tag="PLI30_09580"
                     /EC_number="3.2.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006843908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucosidase BglX"
                     /protein_id="HOI79841.1"
                     /translation="MKGEIMKLSVISMTGVLAVILFASCGSPSAGKSGVGRWVSYSGD
                     KDVEKRVDSVLALMTLEEKIGQMTQFSANWSITGPVMSDDFKPYLEKGLVGSIFNATS
                     VEGIRKMQEVAVEKTRLGIPILFGQDVIHGYKTIFPIPLAEACSWDLEMMRRTAEIAA
                     IEAASDGINWTFAPMVDIARDARWGRVMEGAGEDPYLGSRIAEARVIGFQGGADGKAL
                     GELHTLLATGKHLAAYGAAESGRDYNPAELSEHTLRNIYLPPYKAAIDAGVGTMMASF
                     NEINGVPATADRWLMTDILRGEWDFTGFVVSDYTGINELVPHGVAEDEKQAAELAVNA
                     GIDMDMTGATFIKYLSQSVREGKVSEEVINNAARRILEIKFLLGLFDNPYRYLDEERA
                     KQNTMTPEFMETARKAVASSVVLLKNDNRLLPITKESGKTVALIGPLMNDSINLNGEW
                     AGLGDRNKSVPILKGLMEKYRDTSVKLLFAEGSSITGTTPGKIAEAVSVARRADLVIA
                     AMGEDFNWSGEAAVRTDIRLPEPQRELLKALKATGKPLVLISLSGRPLDLSWEDENMD
                     AILQAWFPGTQAGHGIADVIAGDYNPSGKLVMSFPRNMGQIPIYYNQKNTGRPVDLSN
                     ESTDYKSNYLDTSITPLYPFGYGLSYTTFSISNVSADKSSIRTTGDKMVVTAHVQNTG
                     DIDGELVVQVYVRDLVGSVTRPVKELKGFQKTALKAGETKQVNFELKSEDLAFYGTDG
                     KKKTEAGDFVLWVAQSSADNSNRIRFSANTPDNAR"
     gene            4816..5003
                     /locus_tag="PLI30_09585"
                     /pseudo
     CDS             4816..5003
                     /locus_tag="PLI30_09585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019537973.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
BASE COUNT         1387 a         1178 c         1327 g         1111 t
ORIGIN      
        1 aattattaac ctaaaaataa tatctcacga ttatgaagaa aagaacattt atttatcaac
       61 ttgtattgat aaccgtttta ggattggtga tctcttcctg tttccaggac ttgggccaaa
      121 atccaccgtt cgactatcct gaggagcctc cgaaaccgcc catcagtgaa gacggacaac
      181 tattttacat gacattcaac gacaattata aggatgctag aagtaataaa gaggcttcca
      241 taacaggaaa tcccggattt gcggggggaa aggttgaaaa agcctatgcc ggagctaaag
      301 acagctacct cacctttaag ttatccgata tgccggcagc gcttggaccc gctgtaacag
      361 taggattctg gtataaagta aatgccagtc ccgacagagc aggtatcatc gtgatcggtc
      421 ctgtatcgga aggagcgcct gcaaatgcgc aaaacaacag gacatcgggt ttccggatat
      481 tccgcgaaaa tgcaggggga aaacaacgaa tcaaagccaa tgtaggcaac ggaactgcag
      541 acggctggct tgacggaggg gataaagccg acatcgatcc caccatcttc ccgtggaaat
      601 accttgcgtt ggtattgact gagggaaaag cgtttttcta tatcgacggg gaggaagtag
      661 cctcatccga tttatcaaaa gtaagctggg ccggatgtga cataatgtct atcggatcgg
      721 gagaacctcg tttcaacgaa tgggggcacc tctcggacaa cagtttgatc gatgagctgc
      781 ggatatacaa taaggcgttg actgcatcac agatcaagga aattgtaaat cacgatacgg
      841 aatagtcatg atcagaatat ttttacccct tatcactctg ctcatcctct tgtcgggatg
      901 ttccgacaag aagaacgaac aaaacgaaac tcccaaaccg gaaacacgcc cgctcacttt
      961 cgcttccgat gaggcgtttc tcgactttat ccagcaagcg cacatcaatt atatgtggga
     1021 cggagcggaa aagacttcag gattagctcc ggaacgcatg catatcgatg ggaactatcc
     1081 cgaaaacgat gccgatgtaa ttaccaccgg aggcagcggt tttggaattg ccggacttgt
     1141 tgtcgggatt gagcgggggt ttattccacg taccgacggc gtagcacggt tacaccgaat
     1201 tgtcgattac ctgacaaaag ccgaccgatt ccacggcata tggcctcatt ggattcatgg
     1261 cccaaccggc aaagtgaaac cgtttgggct aaaagacaac ggtggcgacc tggtggaaag
     1321 tgcttttctg atgcagggat tgctttgcgt ccgggagtat ttcaaggacg ggagtgagaa
     1381 tgagaagcaa ctggccacca aaatcgacgg actttggcgg gagatggaat gggactggta
     1441 cctcaacggc aaagatgtat tatactggca ttggagtccc gaatacggat gggagatgaa
     1501 ttttccgctg gagggttaca acgagtgcct cattacctat atcctggccg catcctcacc
     1561 tacacacccg atctccgctt cggcctatca caacggctgg gcccgtggtg gagctatccg
     1621 gagcagtgcc gtggcctacg ggttgccact tattctcaaa cacaacggtg ctgaagagta
     1681 cggcggccct cttttctggg cgcactattc gtatatagga ctgaacccga aaggattaaa
     1741 agaccaatac gccgattact gggaggttaa tacaaaccag gcacgtatca attataacta
     1801 ttgtaaacaa aatcccaaag gattccaggg atacggggag aattgctggg ggttgaccgc
     1861 cagctactcg gtgaacggat actcggcgca tatgcctgta aagaatgatt tgggcgtcat
     1921 aacaccaacg gcggctttat ccagttttcc ttatacgccc gaagaatcga tgaaagcgtt
     1981 aaaacatttc tatgttgaac tgggcgacag tatctgggga aaatacggat tttacgatgc
     2041 attcagcatc caggacaatt ggtatccgca gaggtacctg gccatcgacc aactgaccat
     2101 tgccccgatg attgagaacc accgcagcgg actgctgtgg aacctcttca tgggtgcccc
     2161 ggagatacaa aaaggccttc aaaacctggg atttgaattt ttgaataatt gacttttcgt
     2221 gtgttcttat tgaaacgtat tcagttaaag ttaagacctg taaatgtttc gagagaaact
     2281 cattctgaga gacattgccg gttgaaaatt cagctgcata caaatatgca aaacttgaat
     2341 gaataagata caagactagt aacattgatg aaaggagaga tcatgaagct aagcgttatt
     2401 tcaatgacag gtgtgttggc ggtaattctg ttcgcatctt gcggatcacc ctcagccgga
     2461 aaatcaggtg taggaaggtg ggtgagctac agcggcgaca aagatgtaga gaaaagagtc
     2521 gattcggttc tggccctgat gaccctggag gagaagatcg gacaaatgac acagttctcc
     2581 gctaattggt ccatcacggg cccggttatg tcggacgact tcaagcctta ccttgaaaaa
     2641 ggacttgtag gcagcatttt caatgctacc tcggtagagg gtatacggaa aatgcaggaa
     2701 gttgccgttg aaaaaacacg attaggtatc cctatcctct tcgggcagga tgtaatccac
     2761 ggatacaaga ccatctttcc cattccactg gcggaagcct gttcctggga tctggaaatg
     2821 atgcgtagaa ccgcagaaat tgctgctatt gaagcggctt cggacggaat caactggact
     2881 tttgccccga tggtggatat tgctcgtgat gcgcgttggg ggcgtgtaat ggaaggtgcc
     2941 ggtgaagatc cctacctggg cagcaggatt gctgaagcac gcgtcatcgg atttcagggg
     3001 ggagcggacg gcaaggcact gggagagcta cacaccctgc tggccacggg aaaacacctg
     3061 gcagcttacg gtgccgccga atcgggcagg gactacaacc ctgccgaatt gtcggaacac
     3121 accctcagaa atatttatct cccgccctat aaggccgcaa tagatgccgg ggtgggcacg
     3181 atgatggcat ccttcaacga aatcaacggt gtgccggcaa cggcagacag gtggctgatg
     3241 accgacatac tgcgcggaga atgggacttt accggctttg tggtaagcga ctacaccggt
     3301 ataaatgagc tggtgcctca tggggttgcc gaagatgaga agcaggctgc agagctggcc
     3361 gtcaacgccg gtatcgacat ggacatgacc ggtgctacct ttatcaaata tctttctcaa
     3421 tcggtgaggg aaggaaaagt atcggaagaa gtgatcaaca acgcagcaag gcgtatcctg
     3481 gagataaagt tcctgttggg actgttcgat aatccttacc gctatcttga tgaagagcgc
     3541 gctaaacaga atacaatgac tccggaattt atggaaacgg cacgaaaggc cgttgcctct
     3601 tcggtggtgc tcctgaaaaa cgacaaccgg ctactgccca tcacaaaaga gagcggaaag
     3661 acggttgcgc tgattggccc cctgatgaac gactccatca acctgaacgg tgaatgggcc
     3721 ggcctggggg accgtaataa gagtgtccct attctcaaag ggctcatgga aaaatataga
     3781 gacacttcgg taaagttgct ctttgcagaa ggatcctcca tcaccgggac aacgcccggc
     3841 aagattgccg aagcggtgtc cgtcgcacgt agagcagact tggtgatcgc cgcgatggga
     3901 gaagatttca actggtcggg tgaagccgcc gtacgaaccg acatccgcct gccggagccg
     3961 caacgtgaac tgttaaaagc gttaaaggcg accgggaaac cccttgtgtt gatttccctg
     4021 agcggacgcc ctctcgacct gtcctgggaa gatgaaaata tggatgctat ccttcaggca
     4081 tggttcccgg gaacacaggc cggtcacggc attgccgacg tgatagccgg cgactacaac
     4141 ccctcaggaa agctggtaat gtcgttcccc aggaacatgg gacagatccc catctattac
     4201 aaccagaaaa ataccggcag accggtagac ctttcaaacg aatcgaccga ttacaagtcg
     4261 aactacctag acacctccat cacccccctc tatccctttg gttacggatt gagttacacc
     4321 acattttcca tcagcaacgt gagtgcagat aaatcatcga taaggacaac cggagacaag
     4381 atggtagtta cggctcatgt ccaaaacaca ggcgacattg acggagagct ggtggtacaa
     4441 gtctatgtgc gcgatctggt gggcagcgtc acccgtcccg tgaaagagct gaaggggttt
     4501 cagaagacag ccctcaaagc cggtgaaacc aagcaggtta atttcgagtt gaaatccgaa
     4561 gacctggctt tctacggtac cgacggaaag aagaagacag aggctggcga ttttgtactg
     4621 tgggtagccc agagttcggc agacaacagc aatagaattc gtttttctgc aaacaccccg
     4681 gataacgcga gataacatca ttttaatgaa tcttaagatg ttcatgcgaa tgaattcact
     4741 atttttgtag gttacaaaga ttaaagcatg gacgataaga ctcaacatcc gataaatgcc
     4801 gcgtaccggg gtacactcat ggatacgctg ggcatcacct tcacacacct gtctcccggc
     4861 cgcacggagg cagtaatgca ggtcgataaa cgagtatgcc agccgttcgg tttgttgcac
     4921 gggggcgcca cactggccct tgcggaatcg gtggccggac agggatcgtt aaccctttgt
     4981 gcgccggacg agatggcggc agg
//
LOCUS       DAOSDR010000241         4993 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrimonas sp. isolate AMR_MDS_1826
            NODE_5979_length_4993_cov_3.180437, whole genome shotgun sequence.
ACCESSION   DAOSDR010000241 DAOSDR010000000
VERSION     DAOSDR010000241.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863355
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrimonas sp. (wastewater metagenome)
  ORGANISM  Petrimonas sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Dysgonomonadaceae; Petrimonas.
REFERENCE   1  (bases 1 to 4993)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4993)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:10:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,720
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 35
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4993
                     /organism="Petrimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5979_length_4993_cov_3.180437"
                     /isolate="AMR_MDS_1826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2023866"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            229..2895
                     /locus_tag="PLI30_09590"
     CDS             229..2895
                     /locus_tag="PLI30_09590"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019540872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HOI79842.1"
                     /translation="MSLIKFQISYHTNFGQEIYVCGSIPELGNLDETGALKLTCEGEV
                     WSAETESKTTGQIEYYYFLKEQGKTIRKEWGSHRRLQVEKSKMFFVQDLWKNKPYHGY
                     LYSSAFTESIFFHEKANSTLSYFPNSILLNVICPFVKKGQKLVVSGEIDQLGNWALNK
                     AKPLDYVGNGEWQILLNAKSLPNEFYYKFVIVDEDTRQSIHWEDGGNRILSTQKAKNQ
                     NSVWVEMGLQFHYNYFLFKGTGTAIPVFSLRSESSFGIGDFLDLKKMIDWAALTHQQL
                     IQMLPINDTITTQTWRDSYPYSAISCYALHPIYLGLSAFPLKDQSKMNAFLEEARKLN
                     KLADLDYEKVLSLKKRYTEHLFEQEKDEILSSTAYQVFYEKNRFWLFSYAAYCYLRDK
                     LGTARFTDWGEFAVYNEQKLQQLIEEDPEAKIVTDFYAFTQYLLDKQLGEVQVYAHGK
                     GVALKGDIPIGINRDSIDAWTTGYLFNMDTQTGAPPDDFSFFGQNWGFPTYNWCAMEQ
                     EGYAWWKNRFCKMADYFDAYRIDHILGFFRIWEIPMHSVQGLLGYFSPALPYWPEELN
                     LAGIPFDEERMTKPFIHEAFLPEIFGEYTPEVTAEYLEVSGWQRFNLKKEYDTQRKIQ
                     HFFGGKADLKNLKIRDGLYALCNEVLFVRDRMNHNLFHPRITAQHSYSYRYLDDNVKS
                     AFNRLYDDFYYRRHNYFWREQAMRKLPQLISSTPMLVCGEDLGMVPDSVHSVMNELQI
                     LSLEIQRMPKDPNVSFSDLHSLPYLSVATTSTHDMSPIRLWWKENKEITQKYYNEILN
                     HEGEAPDECSPELCRQIIQQHLNSSAMWVILPWQDWISLSGELSRKDPAEERINVPAN
                     PEHYWRYRMHISLDDLLKKSEFNATIKLMVER"
     gene            2973..3443
                     /locus_tag="PLI30_09595"
     CDS             2973..3443
                     /locus_tag="PLI30_09595"
                     /inference="COORDINATES: protein motif:HMM:NF025613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4348 domain-containing protein"
                     /protein_id="HOI79843.1"
                     /translation="MKINPVVVFLFFVFLFVNCSGNGKGNREQEGKDTAFAQKNRKQT
                     RQEDFYQFLDKFNSKRIFQLERIIFPITVLAPDANRIALEHTEEKIEKRDWELLDLSY
                     DSTYTTRDFDKYTQTVNFRKDTAHVALRGIDNGIYADYYFKLIDGEWFLVTLVE"
     gene            complement(3462..3941)
                     /gene="cdd"
                     /locus_tag="PLI30_09600"
     CDS             complement(3462..3941)
                     /gene="cdd"
                     /locus_tag="PLI30_09600"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019539540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0008655 - pyrimidine-containing compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HOI79844.1"
                     /translation="MKEINLTTKISVYLLEECTETEKKLIEAAKQATKNAYARYSNFK
                     VGAALLLENGEVITGNNQENAAYPSGTCAERTAVFFANANYPDQTIIAIAVAAHHNGG
                     FTKDVVTPCGACRQVLLETETRYKAPIKILMYSDDGIYVVNSIKSLLPLSFGDEMLK"
     gene            3958..4920
                     /locus_tag="PLI30_09605"
     CDS             3958..4920
                     /locus_tag="PLI30_09605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005837422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HOI79845.1"
                     /translation="MKIIFLMSFHYKYRYISVLSLFLICLFAPGWVWGQSRLRVYEEY
                     IDNYSDIAVRHMNDYNIPASITLAQGLLESGAGMSDLARRSNNHFGIKCHRGWRGESV
                     YAADDTPNDCFRKYRKVEDSYEDHAQFLTVGGRYRELFEVSATDYKGWARGLQKTGYA
                     TDRAYANKLIKLIEDYELYRFDNKNYRKGITRRQREENLEQEVARANWTHQPYKTHGL
                     VYVIAVNGDSFAGIAHEFGFKENDLLKFNEVPEGFPLSEGDIVYFQKKKPRADKPYFE
                     HTVQIGESMYSISQKYGIQLRNLYRLNKKSYEYVPEEGDVLRLR"
BASE COUNT         1479 a          932 c         1136 g         1446 t
ORIGIN      
        1 aattgatgat tctgctgttt ctataaactc cgtctgatcg atttctagaa caggaacaga
       61 cgaacaggga acagggtctt cagctaagtg tgatgtctgc aatcgtttgc acgtgataat
      121 gtttaaaaaa aacttattct ggttatctat ccgtttgaaa tagttgtagt tttgttttca
      181 atcggcaggg gcgataaggt cggttacgtt taaagaaaat aagttagaat gagccttata
      241 aaattccaaa taagttatca cacaaatttt ggtcaagaaa tctatgtttg tggttcaatt
      301 cccgagttgg gcaaccttga tgagaccggt gcattaaaat taacctgcga aggggaggtg
      361 tggtctgccg aaacagagag taaaacaacc ggacaaatcg aatattatta tttcttgaaa
      421 gagcagggta aaactattcg aaaagagtgg gggagccatc gtagattaca ggttgagaaa
      481 tcgaagatgt tttttgttca agatttgtgg aaaaataaac cctatcacgg ttacctgtat
      541 tcatcggctt ttactgaaag tatctttttc cacgaaaaag ccaactcaac tttatcctat
      601 tttcccaaca gtattttatt gaatgtgata tgcccttttg tgaaaaaagg gcagaagttg
      661 gttgtttccg gtgaaattga ccaattggga aactgggctt taaataaagc caaaccactt
      721 gattatgtcg gaaacggaga atggcaaata ctcctgaacg ctaaaagcct tcccaatgag
      781 ttttattata agtttgttat cgtagatgag gatacccgcc agtccataca ttgggaagat
      841 ggcggaaaca ggattttatc aacgcaaaaa gcaaaaaacc aaaacagtgt atgggttgaa
      901 atggggttgc aatttcatta caactacttt ttgtttaaag gtacgggaac ggctattcct
      961 gtattttcgc ttcgaagtga aagtagtttc ggaattgggg attttctcga tctgaaaaaa
     1021 atgatcgact gggctgcgct gacccatcag caacttatcc agatgctgcc cattaacgat
     1081 actatcacta cccaaacgtg gagagactct tatccttaca gtgctatttc gtgctatgca
     1141 ttgcatccca tttatttggg actttcggct ttcccgctaa aggatcagtc aaagatgaat
     1201 gctttcctgg aagaagcccg gaaattgaat aaattggctg atcttgatta tgaaaaggtg
     1261 ttgagtttga agaaaagata tacagaacac ctttttgaac aggaaaagga tgaaatttta
     1321 tcatccacgg cgtatcaagt gttttatgaa aaaaaccggt tctggctttt ttcgtacgcg
     1381 gcatattgct atttgaggga taagttaggc acggcgcggt ttaccgattg gggagagttt
     1441 gcagtttata atgaacaaaa gttgcaacaa cttatcgagg aggaccccga ggcaaaaatt
     1501 gtgaccgatt tttatgcttt tactcagtat ttgctggaca aacagttggg tgaagttcag
     1561 gtgtatgctc acgggaaagg ggtcgcttta aagggagaca tacctattgg aataaatcgc
     1621 gatagcattg atgcctggac aacgggctat cttttcaata tggatacgca aacaggagca
     1681 ccgcccgatg atttttcctt tttcgggcaa aattggggat ttcctactta caactggtgc
     1741 gcgatggaac aggaaggtta tgcatggtgg aaaaaccggt tctgtaaaat ggcagattat
     1801 tttgatgctt accgcatcga ccatattttg ggctttttcc gcatttggga gattcccatg
     1861 cactctgtcc agggattatt ggggtatttt agtccggcct tgccttattg gcccgaagag
     1921 ttgaatctgg caggtattcc tttcgatgaa gaacggatga caaagccatt tatacacgaa
     1981 gcatttttgc ctgaaatatt tggtgaatat actcccgaag tgacggcgga gtacctcgag
     2041 gtttccggat ggcagcgctt caaccttaaa aaagaatacg atacacaacg gaaaattcaa
     2101 cacttttttg ggggtaaggc agaccttaaa aatctgaaaa tccgtgatgg gttgtatgct
     2161 ttgtgcaacg aagtattgtt tgtaagagac cggatgaatc ataacctgtt ccacccgcgt
     2221 attacggcgc aacatagtta ttcttatagg tatctcgacg ataatgtgaa aagtgctttt
     2281 aaccggcttt atgatgattt ttattaccgg cgtcacaact atttctggcg cgaacaggcc
     2341 atgaggaaat tgccacaact catttcttca actcctatgt tggtttgcgg agaagacttg
     2401 gggatggtgc ccgatagtgt gcattcggta atgaacgaac tccaaatact cagtctggaa
     2461 attcaacgga tgccgaaaga cccgaacgta tcgttttccg atctccattc attgccttat
     2521 ctttcggttg ccacaacgtc aacccacgat atgtcaccga taaggttgtg gtggaaggag
     2581 aacaaggaga ttacccaaaa atattacaac gagattctta accacgaagg ggaggctcca
     2641 gacgaatgtt ctcccgaatt gtgccggcag attatacaac aacacttgaa ttcgtcggcc
     2701 atgtgggtga ttttaccctg gcaggactgg atatcgcttt ccggcgagct ttcgcggaag
     2761 gatcctgcag aagaacgtat aaatgtgcca gctaatcccg agcattactg gcggtacagg
     2821 atgcatattt cgttggatga cttgttgaag aaaagtgaat ttaatgcaac cataaaattg
     2881 atggtggaaa gataaaatta tgcaggatta atggtgtaaa acccataatt atagtacctt
     2941 tgcaccatca aacgcataat ttaccgctca agatgaaaat aaaccctgtt gttgtctttc
     3001 ttttttttgt tttcttgttt gtaaactgtt ccggtaatgg caaaggtaat cgtgaacagg
     3061 aaggtaaaga cacagcattt gctcagaaaa atcggaaaca aacgagacag gaggattttt
     3121 atcaatttct tgataaattt aattcaaaaa ggatttttca gctggaaaga attattttcc
     3181 ctataacggt attggctcct gatgctaatc ggatagcgtt agaacatact gaggagaaga
     3241 ttgaaaaaag ggattgggag ctgctcgact taagttacga cagtacctat accacccgcg
     3301 attttgacaa atacacgcaa acggtaaatt ttagaaaaga taccgctcac gttgctcttc
     3361 gcggaatcga caacggcata tatgctgatt attattttaa gctgatcgac ggagagtggt
     3421 ttttggttac cttggtggag taaaaaagcg taaactcaaa attatttcaa catttcatcc
     3481 ccaaaactca acggcagcaa actctttatg ctgttgacca cataaatccc atcatcactg
     3541 tacatcaata tttttatagg cgctttgtac cgggtttctg tttccagcag cacctgcctg
     3601 caggctccgc aaggagttac aacatctttg gtgaatcctc cgttgtggtg tgcagcaacg
     3661 gctatggcta tgattgtctg atcgggataa ttggcgttgg caaagaaaac agctgtccgt
     3721 tcggcacatg tccccgacgg gtatgccgcg ttttcctgat tgttgcccgt tatgacttct
     3781 ccgttttcaa gtagcagtgc cgccccgacc ttgaagttgg aataacgtgc atatgcattt
     3841 tttgtggctt gttttgctgc ttcgatcagt tttttttccg tttcggtaca ttcttctaaa
     3901 agataaacgc ttatctttgt ggttaaattt atttctttca ttctgtttga ttgaaatatg
     3961 aaaattattt ttttaatgag ttttcattac aaatatagat atatttcagt tctttctctc
     4021 tttttgattt gtttatttgc tcccggttgg gtttggggac aaagccggtt aagggtttac
     4081 gaagaatata ttgataatta ctccgatatt gccgtgcgtc acatgaacga ttataacata
     4141 ccggcctcca tcacactggc acagggactg ctggagtcgg gagcgggtat gagcgacctg
     4201 gcgagacggt caaacaatca ttttggtatt aaatgtcacc gcggttggcg tggagaatca
     4261 gtgtatgctg cagacgacac tcccaacgac tgtttcagaa aataccgtaa agtggaagat
     4321 tcttacgaag accatgcgca atttcttacc gttggaggcc gttatcggga actttttgaa
     4381 gtatcggcca ccgattacaa aggatgggcg cgaggattgc aaaaaacggg atatgcaacc
     4441 gacagggctt atgccaacaa gcttattaag ttgattgaag attacgaatt atatcgattc
     4501 gacaacaaaa attatcgcaa agggatcacc cggagacagc gagaagaaaa cttagagcag
     4561 gaagtggcac gggctaattg gacgcatcag ccttataaaa cacacggatt ggtttatgtt
     4621 atagctgtga atggcgattc ctttgccgga attgctcacg aattcggttt caaggagaat
     4681 gatttgctca aattcaatga agtacccgaa ggttttccgc tgagcgaagg cgatatcgtt
     4741 tatttccaaa agaagaaacc gagagctgat aaaccctatt ttgagcacac ggtgcaaata
     4801 ggggagtcga tgtacagcat ttcgcagaaa tacggtattc agctaagaaa cttataccgg
     4861 ctgaacaaaa aaagttatga atatgtcccc gaagaagggg atgtgcttag gctcaggtag
     4921 cggaaaattc gacgtgaaaa atctttgttc tataattata aacaacagat aaaatcaaaa
     4981 aataaataaa ata
//
LOCUS       DAOSDR010000242         4917 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrimonas sp. isolate AMR_MDS_1826
            NODE_6086_length_4917_cov_4.161251, whole genome shotgun sequence.
ACCESSION   DAOSDR010000242 DAOSDR010000000
VERSION     DAOSDR010000242.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863355
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrimonas sp. (wastewater metagenome)
  ORGANISM  Petrimonas sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Dysgonomonadaceae; Petrimonas.
REFERENCE   1  (bases 1 to 4917)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4917)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:10:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,720
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 35
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4917
                     /organism="Petrimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6086_length_4917_cov_4.161251"
                     /isolate="AMR_MDS_1826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2023866"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..192)
                     /locus_tag="PLI30_09610"
     CDS             complement(<1..192)
                     /locus_tag="PLI30_09610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008776571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI79846.1"
                     /translation="MGKGNRHIAKKWFLLVLFASYVITISLFTHTHVVNRVTYVHSHP
                     FKKGEKAQHTHTENQLFLLA"
     gene            302..838
                     /locus_tag="PLI30_09615"
     CDS             302..838
                     /locus_tag="PLI30_09615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006841760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohydrolase"
                     /protein_id="HOI79847.1"
                     /translation="MYPLEIILKYYKPGTRLYDIYISHVSDVTGKALHVAQKHPELAI
                     DVKFLEEAAMLHDIGIFKTRAPNIACEGSLPYICHGYLGRELLTMEGFPRHGMVCERH
                     TGTGLSLEAIIQKKLPVPHRDMRPKSLEEKIICFADKFFSKSQLGKEKPLKKIRESLK
                     NHGTHQVETFDEWCELFL"
     gene            929..1513
                     /locus_tag="PLI30_09620"
     CDS             929..1513
                     /locus_tag="PLI30_09620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007659266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - chaperone binding [Evidence
                     IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HOI79848.1"
                     /translation="MKKNNDYTGPEELYEDDMNLEPAREDKEEVEEQKANVEDNLSDE
                     ADPVEELQVKYDDLNDNYLRLHAEFDNFRKRTLKEKADIIKSGGERVLTDMLPFADDF
                     ERALQALHTAEDKEAMVEGMDLIYSKFLNFLNQHGVKEIEALGQPFDADKFEAITTVP
                     VQDKSQKGVIIDCVQKGYQLNDKIIRYPKVIVGE"
     gene            1515..2666
                     /gene="dnaJ"
                     /locus_tag="PLI30_09625"
     CDS             1515..2666
                     /gene="dnaJ"
                     /locus_tag="PLI30_09625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006843326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HOI79849.1"
                     /translation="MATKRDYYEVLEVSKTATAEEIKKAYRKKAIQFHPDKNPGDATA
                     EEKFKEAAEAYEVLSDQNKRARYDQFGHAGMGGAASGGGFGGGMSMEDIFSQFGDIFG
                     GHFGGFGGFGGFGRPGRRTNRGSDLRVKVKLDLKEILNGVEKKIKVKKYVACSHCNGN
                     GSENGNSVSSCSTCNGSGVVTRVQNTILGQMQTTTTCPSCNGEGKTISKKCAHCNGDG
                     IVLDEEVISIKIPAGVEEGMQLSMSGKGNAARRGGVNGDLLILVEEEEDPNLIRDHND
                     VIYNLFVSFPTAALGGTVEVPTIEGVAKVKIDPGTQPGKVLRLRNKGLPTINGYGTGD
                     ELINVNVYIPENLSDAEKKKIEEMDKSPNFAPSKSARKSVFNKFRKMFD"
     gene            2872..4776
                     /gene="acs"
                     /locus_tag="PLI30_09630"
     CDS             2872..4776
                     /gene="acs"
                     /locus_tag="PLI30_09630"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019539306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003987 - acetate-CoA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0019427 - acetyl-CoA biosynthetic process
                     from acetate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HOI79850.1"
                     /translation="MSSYSIKSLEHYFKVYRQSVRKPKKFWSKIAENNFTWYRKWDKV
                     VEYDMERAEFTWFKNAKLNITKNCIDRHLANRSDQAAIIFEPNEPNEPAQHFTYSQLY
                     AEVSKMANVLRAQGVEKGDRVCIYLPMLPELAFSVLACARIGAIHSVVFAGFSANALA
                     TRINDSTCKLLITADGGYRGSKTIDLKRIVDEALHKSPTVEKVLVVKRTHQEIEMREG
                     RDLWLQPLLDEAPPTSRAEMMDAEDPLFILYTSGSTGKPKGMVHTTAGYMVYTAYTFK
                     NIFQYNEGDVYWCTADIGWVTGHSYIIYGPLLNGATTVIYEGTPSYPTHSRFWEVVQK
                     HRVNQFYTAPTAIRSLAREKIEYIQEFRLDSLKVIGSVGEPINEEAWHWYNDHVGNKR
                     CPLVDTWWQTETGGILISPIPFVTPTKPTYASLPFPGIQPVLMDELRNEIERNQQIGH
                     LCIRYPWPGMARTIWGDHQRFIDTYFTAFPGKYFSGDGALRDEVGYYRITGRVDDVII
                     VSGHNLGTAPIEDAINEHQTVAESAIVGFPHEIKGNALYGFVVLKETGLWRDRQNLMK
                     EINQLISDQIGPIAKLDKIQFVSGLPKTRSGKIMRRILRKIAVGESDFGDITTLINPE
                     IVEEIKKGRT"
BASE COUNT         1420 a         1068 c         1249 g         1180 t
ORIGIN      
        1 ggcaagaaga aaaagttgat tttcggtatg cgtatgttgt gctttttctc cctttttaaa
       61 cgggtgggag tgcacgtagg ttacccgatt gacaacatgc gtatgcgtaa aaagcgatat
      121 ggtgattacg taacttgcga acaagacaag cagaaaccat tttttcgcaa tatgtcggtt
      181 gccttttccc ataaccgcgc aaagataaac tttttttatt ttattcgaca agcgttatgt
      241 ggaatatttc caaataacaa ccgtttctct tttcgtaatc ttttgttacc tttgcaaact
      301 tatgtatccg ctcgaaatta tcctcaaata ttacaaaccg ggaacaaggt tatacgacat
      361 ttatatatct cacgtttccg atgtaaccgg caaggcgctg catgtcgcac aaaagcatcc
      421 tgagctggca atagatgtga aatttcttga agaagcagcc atgttgcacg acattggcat
      481 attcaaaacc cgggcaccga atattgcctg cgaggggagc ttgccgtaca tttgtcacgg
      541 ctatttaggc agggaattgc ttaccatgga agggtttccc agacacggaa tggtgtgtga
      601 acggcacaca gggacaggat tgagtttgga agccatcatc cagaaaaaat tacctgttcc
      661 acacagggac atgcgtccta aaagcctgga ggaaaaaatc atctgtttcg ccgataaatt
      721 tttctcgaaa tctcaattgg gaaaagagaa acccttgaaa aaaatccggg agagcctcaa
      781 gaaccacggt actcatcagg tagagacatt tgatgagtgg tgcgagttgt ttctgtaata
      841 gtttggcaaa ctatttgcag tgatgaagtc tgaacgatca agattttttt attgctgaaa
      901 ggcagacaca tatatttttt agatgattat gaagaaaaat aacgactata ccggcccgga
      961 agaattgtac gaagacgata tgaacctgga gccggcccgg gaggataaag aggaggttga
     1021 agaacagaag gccaatgttg aagacaattt gtcagatgag gccgaccctg tagaagaact
     1081 acaggtgaaa tacgatgact tgaacgataa ctatttacgc cttcatgccg agttcgataa
     1141 tttccgcaag cggaccctga aggaaaaggc cgacattatc aaaagcgggg gagaacgtgt
     1201 acttacggac atgcttcctt ttgccgatga tttcgaacgt gcactgcaag cacttcatac
     1261 ggcggaagac aaggaggcta tggtggaagg aatggatttg atttattcga aatttctgaa
     1321 tttcctgaat caacacggtg taaaagaaat tgaggccctt ggacagcctt ttgatgccga
     1381 caagtttgaa gctataacaa ctgtccctgt tcaggataaa tcgcaaaaag gagtgatcat
     1441 cgattgcgtg caaaaaggtt atcagttgaa cgataaaatt atccgttatc cgaaggtaat
     1501 tgtgggggaa taagatggca acgaaaagag attattacga agtactggaa gtttcaaaaa
     1561 cggctacagc tgaagagata aaaaaagcct atcgcaaaaa agccattcaa ttccaccccg
     1621 ataaaaatcc gggagatgct acagccgaag agaaattcaa agaagctgcc gaagcctacg
     1681 aagtactcag cgatcagaac aagcgtgccc gttacgacca gtttggccac gcaggaatgg
     1741 gaggtgctgc atcgggtggt ggctttgggg gcggaatgtc gatggaggat attttctctc
     1801 agtttggcga tatctttggc ggtcattttg gcggattcgg cggattcggc ggatttggca
     1861 ggccgggtag gcgtaccaac cgcggctccg accttcgggt aaaggtgaaa ctagacctga
     1921 aggagattct taatggggtt gaaaagaaga taaaggtgaa aaaatatgtg gcctgttcgc
     1981 actgcaacgg caacggttcg gaaaacggga actctgtttc cagctgttca acctgtaacg
     2041 gatcgggcgt agtaacgcgc gttcagaaca ctattctcgg acagatgcaa accacaacaa
     2101 cctgtccttc ctgcaacgga gagggaaaga caatttccaa gaaatgtgcg cattgcaacg
     2161 gtgatggaat tgttctcgac gaggaagtga tctccatcaa aatcccagca ggtgtggaag
     2221 aaggaatgca gctgtctatg tcggggaaag ggaatgcagc ccgtcgagga ggcgttaacg
     2281 gtgatttact tattttggtg gaggaggagg aggatccgaa cttgatccga gatcacaacg
     2341 atgtgatata taaccttttt gtgagtttcc caacagccgc attgggtgga acggttgagg
     2401 ttcccaccat tgaaggtgta gccaaagtga aaatcgatcc cggaacccag ccgggtaaag
     2461 tgctacgcct gcgtaacaag gggctgccga caataaatgg ttacggtacc ggtgatgagc
     2521 ttattaacgt gaatgtttat attcccgaaa acctgagcga tgccgaaaag aaaaaaatag
     2581 aagagatgga taaatcgccc aactttgccc cgtcgaagtc ggccaggaag agtgtgttca
     2641 ataaattccg gaaaatgttc gattaggaac agaattttca caattacata tccaataaag
     2701 cagcagcagg cctaaaaggt ttgactgctg ttttttttaa caacagcctt aattgttaaa
     2761 acaacgacga aaaaagctta aactgaaaca aaatgggaga aaaatgcgtt gaagtgttat
     2821 aaataataaa tattttttat ttatttgtga ttgttaaaaa tccagcttag tatgagtagt
     2881 tattccatca aatccttaga acactatttt aaggtgtatc ggcagtcggt ccgtaaaccg
     2941 aagaaattct ggagtaagat agcagagaac aatttcacct ggtaccggaa atgggataaa
     3001 gtggtggagt atgacatgga gagagcggaa ttcacctggt ttaagaatgc caagctcaac
     3061 attaccaaga attgcatcga ccggcaccta gccaacagga gcgaccaagc cgccatcatc
     3121 tttgaaccca acgaacccaa cgaaccggcc caacatttta cctacagcca gctctatgct
     3181 gaggtgtcta agatggccaa tgtgcttaga gcccaagggg tggagaaggg cgatcgggta
     3241 tgcatctacc tgcccatgct ccctgaactg gcattcagtg tgttggcttg tgcgcgcatc
     3301 ggggcaatac actccgttgt tttcgccggc ttttcggcca acgcgctcgc cacacgcatc
     3361 aatgattcta cctgtaagct cctgatcacg gcggacggag gctatcgcgg atcgaagacc
     3421 atagacctta aacggatcgt ggacgaagcc ctccataaat cgcctaccgt tgagaaagtg
     3481 ctggtggtaa agcgtaccca tcaggagatt gaaatgagag aggggcggga tctttggttg
     3541 cagccactgc tggacgaagc gcctcctacc agtagggcag agatgatgga cgctgaagat
     3601 cccctgttca tcctgtatac ttccggatct accgggaagc ccaaggggat ggtgcatact
     3661 acggcaggtt acatggttta cacggcgtac acattcaaga atatctttca atacaatgaa
     3721 ggcgacgtct actggtgtac cgccgatatc ggctgggtta ccgggcattc ctacatcatc
     3781 tatggcccct tgctaaatgg ggctaccaca gttatttacg agggaacccc ttcctatccc
     3841 actcacagtc gtttctggga ggtggtacag aaacaccggg tgaaccagtt ttataccgct
     3901 ccaacagcga tccgctccct ggccagggag aaaattgaat acattcagga gttcagactg
     3961 gactcgctca aggtgatcgg ttcggtgggg gagccgatta acgaggaggc atggcactgg
     4021 tacaacgatc atgtgggcaa caagcgctgc ccgctcgtgg atacctggtg gcaaacggaa
     4081 accggaggga tcctgatctc acccatccct ttcgttacac ctaccaagcc gacttacgct
     4141 tctcttcctt ttcccggaat tcaaccggtc ctgatggatg agctgcgaaa cgaaatagaa
     4201 cggaaccagc agatcggcca tctctgcatc cgctatccct ggccgggcat ggcacgaacc
     4261 atctggggtg accaccagcg gttcatcgat acctacttta cggcgtttcc cggtaaatac
     4321 ttctcgggcg atggagcgtt gcgtgatgaa gtgggttatt accgcatcac cggaagggtc
     4381 gacgatgtga tcatcgtctc ggggcacaac ttgggtaccg cacccatcga ggatgcgatc
     4441 aacgagcacc agacagtagc cgagagcgct atcgtgggat ttccgcatga gatcaaaggc
     4501 aatgcattgt acggtttcgt agtgctcaaa gaaaccgggt tgtggcgtga ccggcaaaac
     4561 cttatgaaag agatcaatca acttatctcc gaccagatcg gccccattgc taagctggat
     4621 aagattcagt tcgtaagtgg attaccaaag acccgttccg gaaagatcat gcgtcgcatc
     4681 ctgcgaaaaa tagccgtggg cgaatcagat ttcggcgata tcactacatt gatcaaccct
     4741 gaaattgtgg aagagataaa aaaagggcgg acataaggat gtccagaact actttccgtt
     4801 ttctgattcc gtacaggcca gtatatccca ggaaaacaag gctattcgct cgccgttatt
     4861 gaatgcggac cggttcatcc tgtttattgt ttcgatcaat gaggacactt tgttttt
//
LOCUS       DAOSDR010000243         4916 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrimonas sp. isolate AMR_MDS_1826
            NODE_6088_length_4916_cov_4.169924, whole genome shotgun sequence.
ACCESSION   DAOSDR010000243 DAOSDR010000000
VERSION     DAOSDR010000243.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863355
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrimonas sp. (wastewater metagenome)
  ORGANISM  Petrimonas sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Dysgonomonadaceae; Petrimonas.
REFERENCE   1  (bases 1 to 4916)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4916)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:10:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,720
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 35
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4916
                     /organism="Petrimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6088_length_4916_cov_4.169924"
                     /isolate="AMR_MDS_1826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2023866"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            154..579
                     /locus_tag="PLI30_09635"
     CDS             154..579
                     /locus_tag="PLI30_09635"
                     /EC_number="1.20.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030612 - arsenate reductase (thioredoxin)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase ArsC"
                     /protein_id="HOI79851.1"
                     /translation="MKILILCTGNSCRSQMAHGFLQSFNPDITVCSAGTEASGKLNPG
                     AVKAMAEVGIDISHHTSDPVGKYLNDAWDYVITVCGGANEVCPAFTGKVKKRLHIGFD
                     DPSHAVGTPEFIESEFRRVRHEIKEAFRKLYDEEIKAQL"
     gene            583..1626
                     /gene="arsB"
                     /locus_tag="PLI30_09640"
     CDS             583..1626
                     /gene="arsB"
                     /locus_tag="PLI30_09640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006843016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008490 - arsenite secondary active
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACR3 family arsenite efflux transporter"
                     /protein_id="HOI79852.1"
                     /translation="MKAKLKILDRYLTLWIFMAMLSGVLSGWLFPSIASFWDSLSVGT
                     THIPIAIGLIIMMYPPLAKVKYEELGDVFKNTRILGLSLVQNWIIGPVLMFFLAILFL
                     QFNVDYMYGLILIGLARCIAMVIVWNDLAHGDRQYAAGLVAFNSLFQILFFSLYAYFF
                     IAVLPGWFGLPTNDSVANITMAEIAKSVLIYLGIPMLAGFLTRYISLKVKDENWYNRK
                     IVPKISPFTLIALLFTIFVMFSLKGEYIVKLPLDVVRIAIPLILYFVIMFVVSFYMSK
                     RAGANYEQSTTLSFTAASNNFELAIAVAIAVFGIHSGEAFAAVIGPLVEVPVMIALVN
                     VAFWFKKRWFRSL"
     gene            1717..3747
                     /locus_tag="PLI30_09645"
     CDS             1717..3747
                     /locus_tag="PLI30_09645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006842191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcdA"
                     /protein_id="HOI79853.1"
                     /translation="MNFKRTITSLFFLFVLSQAFPQHVISWKFSLQDKGNGEIELIAD
                     ATIQQGWHMYDSKIPDNGPYPTSLNFDEIKGAEAVGDFNATGKQATVKYDPVFQMNIG
                     TFDNSARFIQRIKVTDKGSFLITGDVRAQACDDKSCTPPLPNEFSFTSADLPKTVTAT
                     ASTAASETEAPGTAVLPTGNLSQDSSLISSPAAVPISVDKELLWTPVTEELNAFGTQH
                     DGSSSLWSIFLKGLLWGFAALLTPCVWPMIPITVSFFLNRNKKVRRKAVADATLYGAS
                     IIFIYVALGLIITAIFGASALNNIATSAVFNLIFFALLLVFAMSFLGAFELVLPASWT
                     NKMDSKVDSTSGLISLFFMAFTLALVSFSCTGPIIGWLLVEAATQGSYLAPAIGMLGF
                     AIALAIPFTLFAVFPSWLKTLPKSGSWLNSVKVVLGFIVLAVSMKFLSVADLSNGWGI
                     LNRDIFLVLWIVIFGILGFYLLGKLKFHGDSDVPHVTIPRLFLSVFSFAFALYLVPGL
                     WGAPLKPISSILPPLSTQDFKLNEVSLNTVYTDYETGMAVAAQTGKPALVSFGGHGCV
                     NCHKMDATVLVDERVKGLIDNEFVYITLMVDERAKLPEMIEVEENGRKTKLKTVGDKW
                     SYLQRHKFAAQSQPYYVILDHSGKPVAPAHAFDENIVNYVNFLETGLRNFKK"
     gene            3752..4531
                     /gene="mazG"
                     /locus_tag="PLI30_09650"
     CDS             3752..4531
                     /gene="mazG"
                     /locus_tag="PLI30_09650"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019538797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HOI79854.1"
                     /translation="MNTRQQKLEAFGRLLDIMDELREKCPWDNVQTNESLRANTIEET
                     YELAEAIINNSDLEIKKELGDLLLHVVFYAKIGEEKQAFDIADVCHAICDKLIFRHPH
                     VFGDLHADSASFVEKSWEQIKLKEKGGNGTVLEGVPPSLPSLVKAYRIQDKARNSGFD
                     WNKRHDVWDKVKEEIGELETEIENLDKERTEQEFGDVLFSLINAARLYQVNPDNALER
                     TNQKFIYRFNYMEQKVKEQGRSLKEMTLDEMEQIWQEAKRV"
     gene            4528..4737
                     /locus_tag="PLI30_09655"
     CDS             4528..4737
                     /locus_tag="PLI30_09655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005776404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI79855.1"
                     /translation="MKNLLCPQCKIRRFYVLNAVGERLVVTVTEDKEIHPIHEAESLD
                     GFDTSLLYCLGCSWKGTVNMLTDKF"
BASE COUNT         1322 a         1160 c         1130 g         1304 t
ORIGIN      
        1 agatttcaaa aaaggagaaa caggaatttt cagcattacc gtatacggct ataagtcgtt
       61 ataattacat gaaaaatttt gacaatggaa gtccttatat attcaaattc acagtttaaa
      121 agacattaat aacattttaa aaatcaaatc agtatgaaaa tattaattct ttgtacaggg
      181 aacagttgcc gcagtcagat ggctcacggt ttcctgcaat cgttcaatcc ggatatcacg
      241 gtttgttcag caggaacaga ggcatcgggg aaattaaatc ccggtgcggt gaaagcgatg
      301 gcggaagtgg gtatcgacat cagccaccac acatccgacc cggtaggcaa atacctcaac
      361 gatgcgtggg attatgtaat taccgtttgt ggcggcgcta acgaagtgtg tccggcattt
      421 accggaaaag taaagaaaag gctgcatatc ggctttgacg atccgagcca cgccgtcggc
      481 acacccgaat ttatagaaag cgagtttcgc cgtgttcgcc atgagataaa ggaagcattc
      541 agaaaactct acgatgaaga aatcaaagct caattgtaaa gcatgaaagc aaaactaaaa
      601 atactcgacc gctaccttac gctctggatc tttatggcga tgctctccgg agtgttatcg
      661 ggatggctgt tcccctcaat cgccagtttt tgggattcgt tgtccgtcgg aacaacccat
      721 atccccatcg ccatcggact gattatcatg atgtatccac ctttggcaaa agtaaaatac
      781 gaagaactgg gggatgtttt caaaaacacc cggattttag gtttatcgct ggtccagaac
      841 tggatcatcg gtcccgtcct gatgttcttt ctggccattt tgtttttgca gttcaatgtc
      901 gattatatgt acggtcttat cctcatcggg ttggcccgat gcattgccat ggtgatcgta
      961 tggaacgacc tggctcacgg tgaccgtcag tatgccgccg gattggtggc tttcaactca
     1021 ctgttccaaa tccttttctt ttcgttgtac gcctattttt tcatagccgt cctgcccgga
     1081 tggtttgggt tacccaccaa cgactcggta gccaacataa cgatggcgga aattgcaaaa
     1141 agcgtcctta tctatttggg aatccccatg ctggcagggt ttcttacccg ctatatcagc
     1201 cttaaggtaa aggacgagaa ctggtataac cggaaaattg ttcctaaaat cagtccgttt
     1261 acgcttatcg cattgttatt cactattttt gtcatgtttt cactgaaagg cgagtacatc
     1321 gtaaaactgc cgctggatgt ggtccgtatt gccattcccc tgatactgta cttcgtgatc
     1381 atgtttgtgg tttctttcta catgagcaag agagcgggag caaattacga gcagtccacc
     1441 accctttcgt tcacggcagc aagcaataat tttgaactgg ctattgcggt ggccattgct
     1501 gtattcggca tccattcggg tgaagctttt gctgcggtta taggcccttt agtggaagtt
     1561 ccggtaatga ttgcattggt caacgtagcc ttttggttca aaaagagatg gttcaggagt
     1621 ttataactca ttttttcgat ttataaccct gctatccgta agatgtttat tcaactttta
     1681 ctatctttgt gcgttaaacc gtaaaaccac cggataatga atttcaaaag aacaattact
     1741 tcgttatttt ttctctttgt gttatctcag gcatttccac aacatgttat cagctggaag
     1801 ttcagcctgc aagacaaggg aaatggcgaa attgagctga tcgctgatgc aaccatccag
     1861 caaggctggc atatgtacga ctcaaaaatt cccgataacg gaccctaccc cacttcattg
     1921 aacttcgatg aaataaaagg ggcggaagcc gtaggtgatt ttaatgccac aggaaaacag
     1981 gcaacagtta agtacgaccc tgtctttcaa atgaatatcg ggacttttga caactctgcg
     2041 aggttcattc aacgcataaa agttaccgac aaaggtagct tcctcatcac aggcgatgta
     2101 cgcgctcagg cttgcgatga taagagttgc actcccccgc tgcccaatga attttctttt
     2161 acctcggcgg atctgcctaa gacggttacc gcaactgcat caaccgcagc atccgagaca
     2221 gaggctcctg gcaccgcagt tctccctacc ggaaaccttt cgcaggactc ttcactaatt
     2281 tcctcaccgg cagcagtacc catttcggtt gacaaagagt tgctctggac acccgttacc
     2341 gaagaattga acgcttttgg cacacaacac gacggcagct catcgctttg gagcattttc
     2401 ctgaaagggt tgctgtgggg ttttgccgca ctgcttaccc cctgcgtttg gccgatgatt
     2461 cccatcaccg tcagcttctt tctgaacagg aacaaaaaag tccgtagaaa agccgttgcc
     2521 gacgcaaccc tgtacggggc ctctatcatc tttatatacg ttgcgttagg ccttattatc
     2581 accgccattt tcggagcaag cgcacttaac aatatagcaa cgagcgccgt gttcaatctt
     2641 attttctttg cgttgctgct cgttttcgcc atgtctttcc tgggagcgtt tgaactggtg
     2701 cttccggcat cgtggaccaa caagatggac agcaaagtag acagcacctc aggcctgata
     2761 agcctctttt tcatggcttt cacgctggcg ttggtttctt tctcatgcac tggcccgatc
     2821 atcggatggt tgcttgtaga ggctgccaca caaggaagtt acctggcccc cgctatcgga
     2881 atgcttggct ttgccattgc tttagccata ccgttcacgt tgttcgctgt tttcccgtcg
     2941 tggctgaaaa cgcttccgaa atcgggaagt tggctcaact ccgtgaaggt agtgctcgga
     3001 ttcattgtgt tagccgtttc aatgaaattc ttgtcggtag ccgatttgtc aaacggatgg
     3061 ggaattctca accgcgacat attcctggtt ctgtggatcg ttatcttcgg tatcctggga
     3121 ttctacctgc tgggaaaact gaaattccat ggcgacagcg atgttcctca cgtgactatt
     3181 ccccggctgt tcctatccgt cttttcgttt gctttcgccc tataccttgt tccgggattg
     3241 tggggtgcac cccttaagcc catcagttct atcctgcctc cgctatccac acaagatttt
     3301 aaattgaacg aagtctcgct caacacagtt tataccgatt acgagactgg catggctgtc
     3361 gctgcacaaa ccggaaaacc cgcgttagtc agcttcggag gacacggatg cgtgaactgc
     3421 cacaaaatgg atgctacagt tttagtggat gaacgggtaa agggactgat cgacaacgaa
     3481 ttcgtgtaca tcacactgat ggtggacgaa agagccaaat tgcccgaaat gatcgaagtg
     3541 gaagaaaacg ggagaaaaac caaattgaaa accgttggtg acaaatggag ttacctgcaa
     3601 cggcacaaat ttgccgcgca atcgcaaccc tactacgtta ttctggacca ttccggaaag
     3661 ccggttgctc cggcgcacgc tttcgatgaa aacattgtca attatgtaaa tttcctcgaa
     3721 acaggactga gaaatttcaa gaaataaatc catgaacacc cggcaacaaa aactggaagc
     3781 attcggacga ctgctcgaca taatggacga actgcgtgaa aaatgcccgt gggacaatgt
     3841 gcaaaccaac gaaagtctgc gggccaacac catagaagaa acgtatgaac tcgctgaggc
     3901 catcatcaac aacagcgacc tggaaataaa aaaagaactc ggagacttgt tgctgcacgt
     3961 agtgttttat gccaaaatcg gcgaagagaa acaagctttt gatattgccg atgtttgcca
     4021 cgctatctgt gacaagctca tctttcgcca tccgcacgtg tttggagact tacacgctga
     4081 ctcggccagt tttgtcgaga aatcgtggga acaaatcaag cttaaggaaa agggtggaaa
     4141 cggcactgtg ctagaaggtg ttcccccttc cctgccctca ctcgtaaaag cgtaccgcat
     4201 ccaggataaa gcccggaatt caggtttcga ttggaataag cgtcacgatg tgtgggacaa
     4261 agtaaaggaa gagatcggtg aattggaaac cgagattgag aatctggaca aagagaggac
     4321 cgaacaagaa ttcggcgatg tacttttcag ccttattaat gcagccaggc tctaccaggt
     4381 taatcccgac aacgcgctgg aacggaccaa ccaaaaattt atttaccgtt tcaattacat
     4441 ggaacagaaa gtaaaggaac aggggcgttc actgaaagag atgacgctgg acgaaatgga
     4501 acaaatctgg caggaagcga aacgggtatg aagaatctcc tctgtcccca atgcaaaatc
     4561 cggcggttct acgttctgaa cgcggtaggc gaacggttag tggtaaccgt taccgaagat
     4621 aaagaaatac atcccatcca cgaagccgaa tcactggacg gattcgacac ttccctgttg
     4681 tactgtttgg gctgttcgtg gaaaggaacg gtcaatatgc ttacggataa gttctaggcc
     4741 tcacttccct accctccacc gggtaaggat attttttctt ttggcaaaga tgccgttctg
     4801 ttttgtacgc acatcctcaa tcacgtaaaa gatattggca tcaaaaccgg taagcgtatc
     4861 ttcaagggct ttcatttgat tacgatcgat aacggtttca attatatcca cttcat
//
LOCUS       DAOSDR010000244         4914 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrimonas sp. isolate AMR_MDS_1826
            NODE_6092_length_4914_cov_4.375180, whole genome shotgun sequence.
ACCESSION   DAOSDR010000244 DAOSDR010000000
VERSION     DAOSDR010000244.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863355
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrimonas sp. (wastewater metagenome)
  ORGANISM  Petrimonas sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Dysgonomonadaceae; Petrimonas.
REFERENCE   1  (bases 1 to 4914)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4914)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:10:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,720
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 35
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4914
                     /organism="Petrimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6092_length_4914_cov_4.375180"
                     /isolate="AMR_MDS_1826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2023866"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..363)
                     /locus_tag="PLI30_09660"
     CDS             complement(<1..363)
                     /locus_tag="PLI30_09660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI79856.1"
                     /translation="MKQFPKLKEYSDNELIMIVYDDKENWQKEAIIYAKKLLLDRGIS
                     EEYSKERIKEIRNEIEILWKQELKERETESYGVLGLAVMTLFWPKYILRDWHLKRDGY
                     HRMRNQRLFAIGIGFLIYF"
     gene            complement(417..962)
                     /locus_tag="PLI30_09665"
     CDS             complement(417..962)
                     /locus_tag="PLI30_09665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI79857.1"
                     /translation="MKYGYYVILLALAISIAGCATKKSILTSVPQNSNIRLDGKPITI
                     EITDLRNKTTQKQLRIPTITLPGQKDKVHPALTDYQRKLINEQILLHFNQSDPELKVK
                     CTILEGFQAFTAYAFHEREYVQVDIKIELLDPESNILEYGTSTAFFEVKSLDASNEFI
                     NELYHKAIRTAIHDCLIKLAD"
     gene            complement(1166..1834)
                     /locus_tag="PLI30_09670"
     CDS             complement(1166..1834)
                     /locus_tag="PLI30_09670"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase-like protein"
                     /protein_id="HOI79858.1"
                     /translation="MIRKRLIKQNRDLKFNCFLFDWDGCLVDTLPIWFEGMHKGLAHF
                     NIKAPEDMIKKGFQGWDIFTQLGVSDMGVFTKQVYRYINDNLCNVKFNEGVISTLIQL
                     KEKGIKSAVVTTTEREKVFSVLDRLNMMDAFDCVIGRNDVKKLKPDREAIDRALALIE
                     GDKTSTAIVGDSEVDILAGQNAGISTIWFSSAENKNFHPHINEQKLNPDFTINRFHEL
                     QKFF"
     gene            complement(1968..2987)
                     /locus_tag="PLI30_09675"
     CDS             complement(1968..2987)
                     /locus_tag="PLI30_09675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI79859.1"
                     /translation="MEDYKKRYFESLIEEEKNKISENFDTIQKFKEICASKGIQLTDS
                     NFRYHRTIGVVASYPDILLALNSQIKKDKEGLVNCNLLENLFEKKPRAHGFLYDTEFM
                     AMAHPFFRRGMNENANFAPRFVELFWQIKDNKIDLSIALDYNRVRINVSDSTIIELDT
                     WFGAKFNKNIEQISDGTVKLSPPMELDNLDISMFFAGAYSLDISWHTNNEIKTFQAEE
                     FKTENVKLKINNSVFHPVRYLHAEYDLKNKIFRHFDGAIHLYSETEYFQRRDSDFNYN
                     KKSQSPIKADSLKLFKMNGNVNIDSLILFSSHFFTGNPLMIEYFEGQYPEHVKEIIEN
                     IRQTK"
     gene            complement(3166..3414)
                     /locus_tag="PLI30_09680"
     CDS             complement(3166..3414)
                     /locus_tag="PLI30_09680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI79860.1"
                     /translation="MPVQLQSLNLSADRDAWREPDTLWVQRPISRLVCRWSVKPAAGW
                     LKLPKQNRSSLLLPGVHPKLISQPPFLLKAIVLSKPVL"
     gene            complement(3561..4004)
                     /locus_tag="PLI30_09685"
     CDS             complement(3561..4004)
                     /locus_tag="PLI30_09685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI79861.1"
                     /translation="MQKKGKLPLMVLFLVMGGMCFSGCDKNKEEDSLGASRPIPNMLT
                     DLSDGKTGLYTTEWFGDIAAFTLTITFDRTECTFIFTETKTGNKTITKYTYQFAYPTV
                     TLTPEKETERVIIGTVVSGRQLAADEISFVDEQNISAWMRVYRKK"
     gene            complement(4270..4566)
                     /locus_tag="PLI30_09690"
     CDS             complement(4270..4566)
                     /locus_tag="PLI30_09690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI79862.1"
                     /translation="MTKKILIGFALIFALSCNKKNDDDFIKQEKGNIWLSGGLAYCAE
                     QIHLDNGDTLIVNIKDVISYTSGDRVSVKYKELGVNKSCPPGIDCKIVEIKKIK"
BASE COUNT         1334 a          967 c          925 g         1688 t
ORIGIN      
        1 aaaatagatt aaaaagccaa tcccaattgc aaataatctc tgatttctca tcctatggta
       61 tccatctctt ttcaggtgcc aatcccttaa aatatatttg ggccaaaaaa gagtcataac
      121 tgctaaacca agaactccat agctttcggt ttctctttct tttaattcct gtttccagag
      181 gatttcaatt tcgtttctga tttcttttat tctttctttt gaatattctt cagatattcc
      241 ccggtctaaa agaagttttt tggcataaat aatcgcctcc ttttgccaat tttctttatc
      301 atcataaact atcatgatga gttcattgtc cgaatattct tttagtttag gaaattgctt
      361 catctgtctt cttcataatt gcatgtaaca tccggttggg cacagtttgg ttgaggttaa
      421 tccgcaagtt ttatcaggca gtcgtggata gctgtccgaa ttgccttatg gtacagctca
      481 ttaatgaatt cattggaggc atctaaggat ttgacttcaa aaaatgcagt ggaggtaccg
      541 tattctaaaa tattggattc cggatccagc agttcaattt tgatatcgac ttgaacgtat
      601 tcccgttcat ggaaagcata ggctgtaaac gcttgaaagc cttccaggat cgtacatttt
      661 acctttaatt ctggatctga ctggttaaaa tgaagcagaa tctgctcgtt aattaacttc
      721 cgctggtagt cagtcagggc cggatgtact ttgtcttttt gtcccggtaa agttatcgta
      781 gggattctca gctgcttctg cgtcgtttta tttcttagat cagtgatttc gatcgtaatt
      841 ggcttgccgt caaggcgtat gttgctgttt tgcggtacgg atgtcagaat ggactttttg
      901 gttgcacaac cggcaatcga gatggcaaga gccagtagga tgacgtagta gccgtatttc
      961 ataacgttct gtttttgatt cagcactgtg ttaaaatgat gccaacggtc gcaaattggg
     1021 tgcgtgcggg agttcgaaac cacacgcttg tcacacggta gtaaagctaa tgaaatgcct
     1081 ttaacgttcc aaaccagaat cgggctacct gcaattaatt tgctgttctc aaccgtgttt
     1141 ttccctgtaa acctgaaatg tcgaattaga aaaatttctg taattcgtga aaacggttaa
     1201 tcgtaaaatc gggattgagt ttctgttcgt tgatatgagg atgaaagttt ttattttcag
     1261 cagatgaaaa ccaaatagtt gaaattcctg cattttgtcc ggcaaggata tcaacttctg
     1321 agtcaccaac aattgctgtt gaagttttat caccttctat cagagccagt gccctgtcta
     1381 ttgcctcccg gtcgggtttt agttttttta cgtcattcct cccgataacg caatcaaacg
     1441 catccatcat attcaaccta tctaaaacag aaaaaacttt ttctctttcg gttgttgtca
     1501 caacagcaga cttaattcct ttttccttta actggattaa cgtacttatg acaccttcat
     1561 tgaattttac attgcaaaga ttgtcattaa tatagcggta tacttgcttt gtaaagaccc
     1621 ccatatccga aacacccaat tgggtaaaga tatcccatcc ttgaaaccct ttcttaatca
     1681 tatcttcggg tgccttgata ttgaaatgag caagaccttt gtgcatgcct tcaaaccaaa
     1741 ttggaagggt atctaccaaa cagccatccc agtcaaaaag gaaacagtta aattttaaat
     1801 ccctattctg ttttatcaat ctctttctga tcacgaaaag ttttttcaat tcaagttagc
     1861 agcgttggta atggtcactg gtatctggtc gggcaggatt tccaacagaa atcctttcaa
     1921 cccgctaaaa tccccgcctg ccctatacaa tttgttaatg cctgctttta ttttgtttgt
     1981 cttatatttt caataatctc ttttacatgc tcaggatatt gtccttcaaa atattctatc
     2041 atcaatggat tcccagtaaa aaaatgactt gagaacaaaa tcaatgaatc aatatttaca
     2101 ttcccgttca ttttgaaaag tttcaatgaa tccgctttta taggagattg agattttttg
     2161 ttgtaattaa aatcagaatc tcgtctttgg aaatactctg tttctgaata caaatggata
     2221 gcaccatcaa aatgtctgaa tattttattc tttaaatcat attctgcatg taggtatcta
     2281 accggatgaa aaacagagtt gttaattttt aattttacat tttctgtttt aaattcttcc
     2341 gcttgaaagg tctttatttc attgtttgtg tgccacgaaa tatctaatga atatgcaccg
     2401 gcgaaaaaca ttgaaatgtc cagattgtca agttccatgg gggggctcag tttcacagtg
     2461 ccatcggaaa tctgttcaat atttttgtta aattttgctc caaaccatgt gtccaattca
     2521 ataattgttg aatcggatac attaattcgt actctgttat aatcgagtgc gatggataaa
     2581 tcaattttgt tgtctttaat ttgccagaat aactcaacaa atcgtggagc aaaatttgca
     2641 ttttcattca ttcctctcct gaaaaacgga tgagccattg ccataaattc tgtgtcataa
     2701 agaaatccat gtgctcgtgg ttttttctca aatagatttt ccaaaagatt acaatttact
     2761 aatccttctt tatccttttt aatctgtgaa ttaagtgcta ataatatgtc tggataggaa
     2821 gctacaaccc caattgtgcg atgatatctg aagtttgaat ccgtgagttg gattcctttg
     2881 gaagcacaaa tttctttgaa cttttggatt gtatcgaagt tttcagaaat cttattcttt
     2941 tcttcttcaa tcaaactttc aaaatatctt ttcttgtaat cttccatttg taaattgtgc
     3001 agatttttta agcttggcgt taactcattt atacatccat ctcttccgtt tgcaaatacg
     3061 tacaagccgt tattcctgaa taaattgtgg agaaatcgtg gtgggcgagc ggtggataca
     3121 gttgagttga catcaggtta gtccggttta gatttgagct ccaaattaca aaacgggttt
     3181 ggataaaaca atagctttca gcaggaaagg aggctgggaa ataagctttg ggtgaactcc
     3241 cggcaacagg agggagcttc tgttttgttt ggggagtttc aaccatccgg cagccggttt
     3301 gacgctccat ctgcaaacca ggcgactgat tggccgttgc acccaaagag tgtctggctc
     3361 ccgccaggca tctctatccg cgcttaaatt cagcgactga agttggacag gcatacgtta
     3421 cggaagtttc tgctgaacca ggagcctgaa tcggataaaa acctctgctg accaatccag
     3481 gtaacgtttg gctgttgctc tttttaggcc cggcctattg ttcaatgtgt tgtcagaaac
     3541 ttcgctttcc ggctgtaggt ctatttcttt ctgtataccc tcatccaggc cgagatgttc
     3601 tgttcatcca caaacgatat ttcatctgca gccaattgcc tgccactcac gacggttccg
     3661 ataatcacac gctccgtctc tttttccgga gtcagggtaa cggtcggata cgcaaattga
     3721 taggtgtatt tcgtaatggt cttgtttccg gttttggttt cggtgaaaat aaaagtgcac
     3781 tccgttcggt cgaaagtaat cgtcaacgta aaagccgcga tgtccccaaa ccattccgtg
     3841 gtgtataatc cggttttgcc gtctgataag tcagttagca tgttgggaat ggggcgtgaa
     3901 gctcccagcg aatcctcttc cttatttttg tcacatccgc taaaacacat tccgcccatg
     3961 accaggaata aaaccatcag cggcagtttg cctttttttt gcatggtatg ctctttttcg
     4021 gttttttata aaagtagatt tttctggtaa aattaggcat gcaagagcca tttgtttttt
     4081 cgcaatgatg gcatttaaaa tgcgctataa cgcccggtgc ctgttgcaaa tcggattcct
     4141 ctgtctgcca acaggcatgg aagagcgttc ctgttggcga ggacggaacg tcgaagtgag
     4201 aatccgcaat tgcaaaccgc acgaacccca ctttgaaata tgtactttat tagcgagatt
     4261 ggttttgttt tatttaattt ttttaatctc gacaattttg cagtcaatac ctggtggaca
     4321 agatttattt acaccaagtt ctttgtattt cacgctaact ctatctccag atgtgtaaga
     4381 tattacatct ttaatattaa cgattaaggt atctccattg tcaagatgaa tttgttcagc
     4441 acaatatgct aaaccaccac taagccaaat gtttcctttt tcttgtttta taaaatcatc
     4501 gtcatttttc ttattacagg atagagcaaa tattagtgcg aatccgatta aaattttttt
     4561 agtcatagtc gatcagttta tattgtttct ttgatagatt atattttttt caattggttg
     4621 cgtagtatta attaaactgc ccgctaaacg gttgaaaata tgaaatgatg ggttttcgga
     4681 acgtatctgt atccgctgat gagaaacgtt gattgaaaac aaaatgtccg tatgtcactg
     4741 aactctcagt tgttttatat tgtgtcgggt attggtgttc tatgtccata ttttatgatt
     4801 taatatcctc atctgttatt acgccggttc ctaatcaaac aggttgtcaa acggaatttt
     4861 agttaaggat aagatgttat ctcctttttg ggccgctttt acaaaatacc ccgc
//
LOCUS       DAOSDR010000245         4876 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrimonas sp. isolate AMR_MDS_1826
            NODE_6150_length_4876_cov_4.560050, whole genome shotgun sequence.
ACCESSION   DAOSDR010000245 DAOSDR010000000
VERSION     DAOSDR010000245.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863355
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrimonas sp. (wastewater metagenome)
  ORGANISM  Petrimonas sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Dysgonomonadaceae; Petrimonas.
REFERENCE   1  (bases 1 to 4876)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4876)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:10:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,720
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 35
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4876
                     /organism="Petrimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6150_length_4876_cov_4.560050"
                     /isolate="AMR_MDS_1826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2023866"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..330)
                     /locus_tag="PLI30_09695"
     CDS             complement(<1..330)
                     /locus_tag="PLI30_09695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008862898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyltransferase/cytidyltransferase family
                     protein"
                     /protein_id="HOI79863.1"
                     /translation="MDNLESIPSPRLSVTPSRSSRIALFPGTFDPFTIGHESLVRRGL
                     ELVDEIVISIGVNEAKKAYFELEKRMEMIRSLYKDEPRVKVGSYDSLTIEFARKMDAK
                     FILRGIRS"
     gene            complement(371..2227)
                     /locus_tag="PLI30_09700"
     CDS             complement(371..2227)
                     /locus_tag="PLI30_09700"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006842790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HOI79864.1"
                     /translation="MTEFEREQAILNYSEENIVTLEWREHIRRRPGMYIGKLGDGSSS
                     DDGIYVLLKEVMDNSIDEYRMGFGKNIDICIDSGNVSVRDYGRGVPLGKVLDVSSRMN
                     TGAKYDSKAFKKSVGLNGVGIKAVNALSSRFLIESYREGESKSVEYERGHVVNDSPQQ
                     SSSQSNGTVIEFTPDTEIFGDYAYRDEHIEPLLKNYVFLNTGLTITFNGKKFYSKNGL
                     EDLLNDNLTTEILYPIIHLHGDDIEVAITHSNQYGEEYYSFVNGQHTTQGGTHLSAFR
                     EATARVIKEYFTKNFEYSDIRNGMVAAISVKIEEPVFESQTKTKLGSKDMTPDGVSIN
                     KYVSDFLKKELDNYLHKHLETADILQKKILDSEKERKAIAGVTKLARERAKKANLHNR
                     KLRDCRIHYNDSKGNNLDDSCIFITEGDSASGSITKSRDPNLQAVFSLRGKPLNSFGL
                     TKKIVYENEEFNLLQAALNIEDGMDGLRYNKVIIATDADTDGMHIRLLLLTFFLQFFP
                     DLVKKGHVYILQTPLFRVRNKKKTLYCYTEEERLAAIEELGPNPEITRFKGLGEISPD
                     EFKNFIGKDMRLDRVSMRKEDLIKELLEFYMGKNTPDRQTFIIENLVVEEES"
     gene            complement(2322..3452)
                     /locus_tag="PLI30_09705"
     CDS             complement(2322..3452)
                     /locus_tag="PLI30_09705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005859806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI79865.1"
                     /translation="MSLEIRQVKDKDALRQFVQFSIDIYEGNEFFVPPLVYDEIATLS
                     RDKNPAFEHCDAAYFLAYREEKIVGRIAVMINHKANNVWNEKNARFGFVDFIDDPEVA
                     DALFYEAENWARFRGMEKMHGPMGFTDFDYEGLLVDGFDQIGTMQTIYNYPYYVDHIE
                     RLGYKKDQDWYEFLIKIPKEIPERFVRVAEIVKKRFRLNVVKLESKKDVWPYAHEIFE
                     LLNRSYKDLYGFVPLSKAQIDYYVNMYIPMLRLNFLSLVLREEDNRLVGVGIGLPSLA
                     KALQKSGGKFLPTGWYHLYRALKGNDNKVLDLLLIGVDPEYQGKGVNALIFSQFIPEA
                     IRLGFEYAESNPELAVNNKVQSMWDDLEARHHKTRRAFIKDL"
     gene            3703..4629
                     /gene="nusB"
                     /locus_tag="PLI30_09710"
     CDS             3703..4629
                     /gene="nusB"
                     /locus_tag="PLI30_09710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007566761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HOI79866.1"
                     /translation="MINRILIRTRIVQIVYAWYQNTNKDLRTAEKELLFGLQKSYDLY
                     YYLLLLMVELTKAYDARVEAKRNKYLPTDEDLNPNTRLIENKFIGQLSQNHQFNKYLN
                     ERPMSWREHDAFVKNLLDEIIASDIYAEYANETKTDYNDDREFWRKIFKQFILDNEKL
                     EEILEDESIFWNDDIEIVQTFVMKSIRQFSEENGENQRLLPMFKDDEDRQFALKLLHD
                     TILNEQKYRQLIETHSEKWDFDRIAFMDMIIMQIALAEIHTFETIPTSVSLNEYIEIA
                     KSYSTPKSGTFVNGILDAIVADLRKNNIIFKH"
     gene            4657..>4876
                     /gene="yajC"
                     /locus_tag="PLI30_09715"
     CDS             4657..>4876
                     /gene="yajC"
                     /locus_tag="PLI30_09715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019538420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HOI79867.1"
                     /translation="MDILLQAASGASGGMGTSLLMMVAIIAVFYFFMIRPQQKKQKDV
                     QKAREAMKVGDKVVTAGGIHGRIKEIGET"
BASE COUNT         1383 a         1114 c          948 g         1431 t
ORIGIN      
        1 cgagcgtatt ccccgtaata tgaactttgc atccattttc cgggcaaact cgatggtgag
       61 agaatcgtag ctcccaactt tcacccgggg ttcatccttg tataagcttc tgatcatctc
      121 catccgcttt tctaactcga agtaagcctt ttttgcttcg ttcacaccaa tggaaattac
      181 aatttcgtct accaactcca atccgcgccg taccagcgac tcgtgcccga tagtgaacgg
      241 atcaaaagtg cccgggaata atgcaatccg gctacttctc gacggggtga cagatagacg
      301 gggagagggt attgattcta aattgtccat tttccggggt tatttatcat atttacaatt
      361 attccacaag ctacgactcc tcttcaacaa ccaggttttc tatgataaaa gtctgccggt
      421 cgggagtatt tttacccata tagaactcaa gaagctcttt gataagatct tcttttcgca
      481 tggataccct gtcaagccgc atatcttttc cgataaaatt cttgaactcg tcgggagaaa
      541 tttcaccaag acccttaaat cgggtaattt caggattagg accgagctct tcgatggcgg
      601 caagccgttc ctcctccgta tagcaataca gggttttctt tttgttacgc acacggaaaa
      661 gaggtgtttg aagaatataa acatgccctt tttttaccag atcggggaaa aattgcagga
      721 aaaacgtgag caacaacagg cgaatgtgca taccgtcagt atcggcatcg gtagcaatga
      781 taaccttgtt gtagcgcaat ccatccatcc cgtcctcgat attaagggca gcttgcaata
      841 agttaaactc ctcattttca taaacaattt tttttgtcaa cccaaaagag tttaacggct
      901 tgccccgaag gctgaaaaca gcttgcaaat tagggtctct acttttggtg atggaaccgc
      961 tggctgagtc accttccgtg atgaagatac aggaatcatc gagattattc ccttttgaat
     1021 cgttataatg gatgcggcag tcccgcagtt tccggttgtg caagtttgct tttttcgccc
     1081 gttcccgggc taattttgtt accccggcaa tggcttttcg ttctttttca gaatcgagaa
     1141 tcttcttctg aaggatatcg gccgtctcca ggtgtttgtg caggtagtta tccagctcct
     1201 tcttcaggaa gtcgcttacg tatttattga tcgaaactcc atcgggggtc atgtcctttg
     1261 atccgagttt tgtttttgtc tgcgactcaa aaaccggctc ttcgatcttc acgctgatgg
     1321 cagccaccat cccgttccgg atatcggaat attcgaaatt tttggtgaaa tattctttga
     1381 taacccgggc agttgcctca cgaaaagcgg aaagatgcgt cccgccttgt gtggtatgct
     1441 ggccgttgac gaaagagtag tattcttcgc catactggtt ggaatgagta atcgccactt
     1501 caatgtcatc accgtgaaga tggatgatgg gataaagaat ctcagttgtc aggttgtcgt
     1561 tgagcaggtc ttcgagcccg ttttttgaat aaaacttctt tccgttaaac gtaatggtca
     1621 gccccgtatt caggaaaaca taatttttaa gcagaggctc gatatgttcg tcacgataag
     1681 cataatcccc aaaaatttcg gtgtcgggag taaactcgat aacggttccg ttgctttgcg
     1741 aagaagattg ctgcgggcta tcgtttacta catgtccgcg ctcatattca acactctttg
     1801 attcaccttc cctataactt tcgatcagga acctcgaaga caaggcgttt accgctttga
     1861 tcccaacacc gtttaatccc acggattttt taaacgcctt ggagtcgtac ttggctcccg
     1921 tgttcatccg cgaggagaca tccaacacct ttcccaacgg cacaccccgt ccataatcac
     1981 gcacacttac attcccggaa tcaatacaga tgtctatgtt tttcccaaaa cccatccggt
     2041 attcgtcgat ggaattatcc attacttctt tcagcaggac atatataccg tcgtctgacg
     2101 aagaaccgtc tcccaatttt ccaatataca ttccgggacg tcggcggata tgttcacgcc
     2161 actccagggt tacaatgttc tcttcactat aattcagtat cgcctgttct ctttcaaact
     2221 cagtcacttg ttctctttct cctataattt ttcaggtgca gggcctgttt taaaaccttt
     2281 ctgcacttct taaagtcaaa atttgtttct tcttccgata attataaatc ttttataaaa
     2341 gcacggcgcg ttttatgatg tctcgcctcc agatcatccc acatggattg cactttattg
     2401 tttactgcca actcggggtt gctttccgca tattcaaatc ccagccgaat agcttcgggg
     2461 ataaactgac taaagataag ggcattcaca cctttgccct gatactcagg atcaacgccg
     2521 atcagcagca ggtcaagaac tttattgtcg ttccctttca aagccctgta taaatgatac
     2581 cacccggtag ggaggaactt tccgcccgat ttctgcagtg cttttgccag actgggtaat
     2641 ccgattccca ctcccacaag ccgattgtct tcttcacgca gcacgagcga caggaagttc
     2701 aaacgcaaca taggaatata catgtttacg taatagtcta tctgcgcttt ggacaacgga
     2761 acgaaaccgt ataaatcttt ataggaacgg ttcaacaact caaatatctc atgcgcataa
     2821 ggccagacat cctttttact ttcaagcttg acaacattta gtcgaaaacg tttttttacg
     2881 atttcagcaa cacgcacaaa ccgttcggga atttccttgg gtatcttgat cagaaactca
     2941 taccagtcct gatccttttt gtagcccaat ctttctatat gatctacata gtaggggtag
     3001 ttataaatgg tttgcatggt accaatctga tcaaagccat ctaccagcag accctcgtaa
     3061 tcgaaatcgg taaatcccat aggtccgtgc atcttttcca taccgcgaaa acgagcccag
     3121 ttttcagctt catagaaaag cgcatctgcc acttccgggt catcaatgaa atcgacaaaa
     3181 ccaaaacgag cgttcttttc gttccagaca ttattcgctt tatgattgat cattaccgca
     3241 atcctgccaa ctattttttc ttcccggtaa gccaagaaat aagcagcatc gcaatgctca
     3301 aaggccggat ttttatcccg gctcaacgtg gcaatttcat cataaacgag cggcggaaca
     3361 aagaattcgt ttccttcgta tatatcgata ctgaactgaa caaactgtct cagcgcatct
     3421 ttgtctttta cttgtctgat ttctaatgac atactcttta taattttcgc gtatatgaat
     3481 aattgatggt ttaattttca caaaatacta taaacggcaa agataatgaa ttttagagaa
     3541 tgtttgaaac atgatttcag aaaaaacaat atctctaccc gggtgtttat atctatttgt
     3601 atataagttg ttttgtcttc aatcaaatag ttttcaaaac cattttactt gtatatccaa
     3661 aaaaatggct aattttgcat tcttaaaatg ctcatttaaa aaatgattaa tcgaatttta
     3721 attcgtaccc ggatagtaca gattgtttac gcctggtatc agaatacgaa taaagattta
     3781 cgtacagccg aaaaagaact tctgttcgga ttacaaaaat cctacgatct ttattactac
     3841 ctccttttgt taatggttga gctgacaaaa gcttacgacg cccgcgtaga agccaagcgg
     3901 aacaagtatc ttcctacgga tgaggattta aatcccaaca cccggttgat cgaaaacaag
     3961 tttatagggc aactttcgca aaaccatcag ttcaacaagt acctaaatga gcgccccatg
     4021 tcttggagag aacatgatgc gtttgtaaag aacctgctcg acgaaatcat cgcatccgat
     4081 atttatgctg aatatgccaa cgaaacaaaa acggattaca acgacgacag ggagttttgg
     4141 cgaaaaatct ttaagcagtt tatcctcgac aacgaaaaac tggaagaaat tctggaagat
     4201 gaaagtattt tctggaacga tgatattgaa attgtacaga ctttcgtaat gaaatccatc
     4261 cggcaatttt cggaggaaaa cggagaaaat caacgtctgc tgccgatgtt taaagacgat
     4321 gaagaccgtc aatttgcatt gaaattgctt cacgacacca tccttaatga acagaagtac
     4381 cgtcaactaa ttgaaaccca cagtgagaag tgggatttcg accgtattgc atttatggat
     4441 atgattatca tgcaaattgc attggcagag atccatacct ttgaaacgat ccccaccagc
     4501 gtatcgctca acgaatatat cgaaattgca aaatcttaca gcactcccaa gagcggcacc
     4561 tttgtcaacg gaattttaga cgcaattgtt gccgatcttc gaaaaaataa tattattttt
     4621 aaacattaat ttcaaacact ctaaaacgtt attcgtatgg atattttatt acaagcagct
     4681 tcaggtgcaa gtggaggaat gggaacatcg ttattgatga tggtggctat cattgccgta
     4741 ttttatttct tcatgatccg cccacaacag aaaaaacaaa aagacgtgca gaaggctcgc
     4801 gaagccatga aggtgggcga caaagtggtt actgccggcg gtattcacgg acgcataaaa
     4861 gaaattggcg aaacct
//
LOCUS       DAOSDR010000246         4851 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrimonas sp. isolate AMR_MDS_1826
            NODE_6186_length_4851_cov_3.247915, whole genome shotgun sequence.
ACCESSION   DAOSDR010000246 DAOSDR010000000
VERSION     DAOSDR010000246.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863355
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrimonas sp. (wastewater metagenome)
  ORGANISM  Petrimonas sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Dysgonomonadaceae; Petrimonas.
REFERENCE   1  (bases 1 to 4851)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4851)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:10:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,720
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 35
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4851
                     /organism="Petrimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6186_length_4851_cov_3.247915"
                     /isolate="AMR_MDS_1826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2023866"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2737
                     /locus_tag="PLI30_09720"
     CDS             <1..2737
                     /locus_tag="PLI30_09720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010266448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HOI79868.1"
                     /translation="GHYLGDHKGGFTSFYMDATEAIRFGEENVIVVAVNNALNDRYNI
                     PPMNAGNWSVYGGIVRDVRLVITDRLNVPYQGSYKHEGGTFITTPFVSSKSADVQVKT
                     YVQNNFPEARETRVVTYLANSNNIVFDRQESRRMIRPGEIAEFVQHSAPVKNPNLWSP
                     QTPYIYNAYTEVYDGKRLADTYHSTFGIRSIAWDYDLHRLILNGEVTHLHGINRHEEY
                     IWLGQAFPKWIAERDLNDIAKGLEVNYMRTAHYPNAPYVYYFMDQNGICINEELPNIK
                     NQEFDPVVQEQNCREMIRRDRNHPSIIIWSMGNETDHACDSRYAWEEDKSRIITVRQP
                     YNDSYNPVYCPHTDAEMPVESFLRCTIRGWYDVDDRNQEPDDQQWAGTDNWQHVKSNE
                     YNMISNHNGTVWLYADHGADREYVGAPLKHVNPKGWVDSWRTPKYVYYLWQANFAKKP
                     MVFIMPHFWRSQYIGQKKDFTVDSNCEQVELFVNGKSQGILYPTKQNNYNVTFKDILV
                     VQGTIEAVASRKDGTRVTDKVVMAGEPAALTIVPTATRMMSSPDNIIEFRVDAVDKNG
                     VHVYGANPALKFHVEGPAKLIGPEIYLSDRDKTEEYEGTMYIDVPVTNLIRAVGKTGK
                     VKITVSATGLDPASTEIDVVAYQDPAPVSGIVEPVVDFSKSKPVSINTMQANYIKAPV
                     EMLPYQDEISFPIADQAKFRELASDYVVKRNPTIDTRSPEFGYVLDAFEAILNSTARY
                     TGKRGYVVADDSNFLAGQYNTSRAITKNISSKNLPRAYKDMLIEYYAHMIVAKGKDKN
                     YLSENYLIDRIPNGGTAIVIASKGDRQGVKYLNETDLKKVMPLLYPEFASLSQDKQKE
                     AMLLVTRINPGATFTSVRDKKTKNRTDFFTLAPGSVILIPDFKALTKSEFPDKEL"
     gene            complement(2862..4289)
                     /locus_tag="PLI30_09725"
     CDS             complement(2862..4289)
                     /locus_tag="PLI30_09725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010266457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylxylan esterase"
                     /protein_id="HOI79869.1"
                     /translation="MRRHYVFFLLAMTAIPLIAQEPEVEIYTKPLTEVLKQVEERYGV
                     QLIYEEKNVQNRLVHYADWRFYAGLEATLDNILKSQELRWDKKPDGTYEIKKWEYFRK
                     PFEEGRIHLEQLWASYGGLTGWEKRKTLLRDNILRQMKLLPFPKLTPLAPIVTNKRIY
                     DGYSVENVALEVLPGVYLSGSLYKPTKKSRKYPAMLSPHGHFYNKIDKMILNERGRYR
                     EDQQYRCAVLAQMGAYVFSYDMFGWGESDLQFPHKENHRTGLALTMQTWNSMRVIDFL
                     CSLPEVDQSRIGITGASGGGTQTLLAAALDDRISLSIPVVMTSSHFFGGCPCESGLPI
                     HIIPHGINSNNAEIAAMMAPKPQLLISDGKDWTVTTPDIEMPYLKKVYALFNKENEVQ
                     NVHLPDDDHDYGINKRLPMYHFVAQHFKMDISRVCDKNGTIDESRVTIEPAVKMLVFG
                     AERQLPENAVKEADRVWEIIRSLQQ"
     gene            complement(4451..>4851)
                     /locus_tag="PLI30_09730"
     CDS             complement(4451..>4851)
                     /locus_tag="PLI30_09730"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HOI79870.1"
                     /translation="LIITPNPEEEESGDRKFINQVMIVIEKNMSNESFNLQEFAKAMN
                     SSKSVLHSRLQNLTQQSPIEFLRTVRLKKAAQLLALNAYNITQVSYMVGFSDSRYFST
                     CFKKQFGMTPREYIKKYKQEENIEDQLKSI"
BASE COUNT         1294 a         1088 c         1178 g         1291 t
ORIGIN      
        1 cgggcattat ctcggcgacc acaaaggagg atttaccagt ttttacatgg atgccaccga
       61 ggctatccgg tttggtgaag aaaatgtgat tgttgtggcc gtcaacaatg cccttaatga
      121 ccgttacaat atcccgccca tgaatgccgg gaattggagt gtttatggcg gaattgtccg
      181 ggatgtgcgg ttggtgataa ccgatcgcct gaacgtaccc tatcagggat cttacaaaca
      241 tgaaggaggt acttttatta caacaccttt tgtgagttcg aaaagcgctg atgtacaagt
      301 aaagacctac gtgcagaata attttccgga ggcccgggaa acccgggtgg ttacttatct
      361 ggctaatagc aacaatattg tgttcgacag gcaggagagc cgcaggatga tcaggccggg
      421 agaaattgct gaatttgtac aacattcagc gccggtgaaa aacccaaatc tttggtcacc
      481 ccaaactcct tatatttaca atgcttacac cgaggtttat gacgggaaac ggctggctga
      541 cacctatcat tctacgttcg ggatccgttc aattgcctgg gattacgatc tccaccgcct
      601 gatccttaat ggcgaagtca cccatcttca cggcatcaac cgacatgaag agtatatctg
      661 gctgggacag gccttcccta aatggattgc cgaacgcgac ctgaacgata ttgcaaaggg
      721 actggaagtg aattatatga gaacagccca ctatccaaat gcgccttatg tatattattt
      781 tatggatcag aacggaatat gcattaatga agagcttccc aatatcaaga accaggagtt
      841 cgatccggtc gtacaggagc agaattgccg tgaaatgatc cgtcgcgacc gaaaccatcc
      901 gtcaattatt atctggagta tggggaacga aacggaccat gcctgcgatt cccgttatgc
      961 ctgggaagag gataaaagcc ggatcatcac ggtgagacag ccctataacg actcttataa
     1021 tccggtatat tgtccgcata ccgatgccga aatgcccgta gagagttttc tgcgttgtac
     1081 cattcggggt tggtatgatg tggacgaccg caaccaggag cccgacgacc agcaatgggc
     1141 gggtaccgat aactggcagc atgttaaatc gaatgagtat aatatgattt cgaaccacaa
     1201 tggtaccgta tggctctatg ccgaccacgg cgctgaccgg gaatatgtgg gagcccctct
     1261 gaagcatgtc aatcccaaag gatgggtcga cagttggcgg actccaaaat atgtttatta
     1321 tctctggcag gcgaattttg ccaaaaagcc gatggttttc atcatgcccc acttctggcg
     1381 cagtcaatac atcgggcaaa agaaagattt tacggtagat tcgaactgtg aacaggtgga
     1441 gcttttcgtt aacggtaaaa gccaggggat cctttatcct acaaaacaga acaattacaa
     1501 tgtgacattc aaagatattc tggttgtaca aggaacaatt gaagcggttg cttccagaaa
     1561 agatggcaca cgggtaaccg acaaggtggt tatggcgggt gagccagccg ccttgaccat
     1621 tgtcccgacc gcgaccagaa tgatgtcttc tcccgataat atcattgaat tcagggtgga
     1681 tgccgtagat aaaaacggag tacatgtgta cggtgccaac ccagcgctga aattccatgt
     1741 ggaaggccct gcaaaattga ttgggccgga gatatacctc agcgaccggg ataaaacaga
     1801 ggagtatgaa ggcacaatgt atattgatgt cccggtgacc aacctgattc gggctgtcgg
     1861 taaaaccggc aaagtgaaga ttaccgtttc ggcaaccggc ctcgatccgg catcaaccga
     1921 gattgacgta gttgcttacc aggatccggc accagtgtca gggattgtgg agcccgttgt
     1981 ggacttttct aagtcaaaac cggtatccat caataccatg caggcaaatt atatcaaagc
     2041 gcctgtggaa atgcttccct atcaggatga aatcagcttc ccgattgctg accaggccaa
     2101 attccgggaa ctggcatccg attatgtcgt gaaacgaaat ccgactattg atacccgttc
     2161 tccagagttc gggtatgtac ttgacgcatt cgaagcgatt ctgaattcaa cggctcgata
     2221 taccggaaaa agaggctatg ttgtggctga tgattccaat tttctggccg gacaatacaa
     2281 cacttcccga gcaataacga aaaacatctc ctcgaaaaat ctgccgcggg catataaaga
     2341 tatgctgatc gaatattacg cccacatgat cgtagccaaa ggcaaagaca aaaactatct
     2401 atcggaaaat tatttgatcg atcgtattcc aaacgggggg acagccattg taatagcttc
     2461 aaagggagat cggcaggggg tcaaatacct caatgaaacc gatttgaaga aggttatgcc
     2521 attgctttat cctgagtttg cttctctgtc tcaggacaaa caaaaggagg ccatgttgct
     2581 ggttacaaga attaatcccg gggcgacatt tacaagcgtc cgggataaga agacgaaaaa
     2641 tcgaaccgat tttttcactc tggcgcctgg aagtgttatt cttattcccg acttcaaggc
     2701 attgacaaaa agtgagttcc ctgacaagga gctttaaaaa cgagctgttt taatgttgta
     2761 aaatagccgg aagagttaat ttcagacact tccggctatt ttgattgcaa gatctaaggt
     2821 gaagtcaaac tgctatttcc ggtggttcgc agggctgcct attattgctg aagtgatctt
     2881 ataatttccc aaaccctgtc cgcctctttt acggcattct ccggcagctg cctctcagcg
     2941 ccaaaaacca gcattttaac ggcaggctcg atggtaaccc ggctttcgtc aatagttccg
     3001 tttttgtcac agacccgcga aatatccatt ttgaaatgct gtgctacaaa atggtacatt
     3061 ggcagccgct tgttaatgcc ataatcgtga tcatcgtctg gcagatgtac attctgtacc
     3121 tcgttctctt tgttgaaaag ggcgtaaact tttttgagat acggcatctc tatgtcgggg
     3181 gttgttaccg tccagtcctt tccgtcggag attaacaatt gtggttttgg agccatcatg
     3241 gctgctatct cagcattatt gctgttgatt ccatggggga tgatatgaat tggcaacccg
     3301 ctttcgcagg gacatcctcc aaaaaaatga gaagaagtca ttactaccgg gatacttagc
     3361 gaaatgcggt catccaatgc tgctgccagc agggtttggg ttccgcctcc ggatgctccg
     3421 gtgattccga tccggctttg atcaacctct ggaagcgagc agagaaagtc gatcacccgc
     3481 atactgttcc aggtttgcat ggtcagcgct aatccggtgc ggtgattctc tttgtggggg
     3541 aattgcaggt cggattctcc ccagccgaac atgtcgtagc tgaagacata ggcgcccatc
     3601 tgggccaaaa cggcacaacg gtattgctgg tcttcccggt atcttccccg ttcattcaga
     3661 atcattttgt caatcttgtt gtagaaatgt ccatgcggag agagcatcgc cggatattta
     3721 cggctttttt tcgttggctt gtacagggaa ccagaaagat aaactcccgg aagaacttcc
     3781 agcgccacat tttctacgct gtagccatcg taaatccgtt tgtttgttac aattggggcc
     3841 agcggggtca gcttggggaa tggcaataat ttcatttgcc ggagaatatt gtcccgcaat
     3901 aaggttttgc gcttctccca gccggtcaat ccaccataag aagcccaaag ttgttccagg
     3961 tggatgcgtc cttcctcgaa tggtttgcgg aaatattccc attttttgat ttcgtaagtt
     4021 ccatctggtt ttttgtccca acggagctcc tgggacttca gaatgttgtc aagagttgct
     4081 tccagtccgg cataaaaacg ccagtctgca taatggacaa gccggttctg aacgtttttt
     4141 tcttcgtaga tcaattgtac gccataacgt tcttcgacct gtttcaggac ttcagtcaat
     4201 ggtttggtgt aaatttcaac ttcaggttcc tgggcaataa gtggaatagc tgtcattgcc
     4261 agcagaaaaa atacgtaatg tcttctcatc gtaatattag cttatagttg ttacagtgtc
     4321 tgataacaaa aatagcggtc tggcagcaag tatgaccggc agaggcggat attcaacata
     4381 ctcagaaaaa atccgaacat ttccctgccg aagaggcacg gcgctcatgg aacaaaaaag
     4441 ggagatgtga tcagatgctt tttagttggt cttcaatgtt ttcttcctgt ttgtattttt
     4501 taatatactc acgaggagtc attccgaact gttttttgaa acaggtactg aaataacggg
     4561 agtcggaaaa gccaaccata tagctgacct gggtgatgtt gtatgcgtta agcgccagca
     4621 attgagcggc ttttttcagg cgtacggtcc gcagaaattc aatcggggat tgttgcgtga
     4681 ggttttgcag gcgggagtgg agtacggatt tgcttgaatt catcgccttg gcaaactcct
     4741 gtaaattaaa actttcgttt gacatgtttt tttcaattac aatcatcacc tggttgatga
     4801 acttccggtc tcccgactcc tcttcttccg gatttggcgt gataatcagg t
//
LOCUS       DAOSDR010000247         4842 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrimonas sp. isolate AMR_MDS_1826
            NODE_6200_length_4842_cov_3.752872, whole genome shotgun sequence.
ACCESSION   DAOSDR010000247 DAOSDR010000000
VERSION     DAOSDR010000247.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863355
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrimonas sp. (wastewater metagenome)
  ORGANISM  Petrimonas sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Dysgonomonadaceae; Petrimonas.
REFERENCE   1  (bases 1 to 4842)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4842)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:10:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,720
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 35
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4842
                     /organism="Petrimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6200_length_4842_cov_3.752872"
                     /isolate="AMR_MDS_1826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2023866"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..348
                     /locus_tag="PLI30_09735"
     CDS             <1..348
                     /locus_tag="PLI30_09735"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HOI79871.1"
                     /translation="KHNLNTAHIKKRKVQLELDEAREMIDLQIRQNRYKYNESIRKAE
                     LTQLSKAQAEENMKIAKENLLEGRTRLTELLEAQVQWENASSEYIDALIEIKTTRSEL
                     EKSTGEIYKYVGN"
     gene            399..1454
                     /locus_tag="PLI30_09740"
     CDS             399..1454
                     /locus_tag="PLI30_09740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004331990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HOI79872.1"
                     /translation="MQTKRTILYATCFAILLMGGCSKKTGSNNAEINEKKAVMATVKE
                     VVEMEYTPVLNFTGTAEANKEVNLGSAIPGRVERINFPKGSFVSKGAVIAEMSDEMLI
                     QAQIELEAIRKDFERISRLKEKGSVSVMDYDHIKAKFEASQTKVEMLKKNTSIIAPFG
                     GVLVDIMVNEGENYSFVPSVSPDLKVKSGIATLMQLNPLKVKIEINEKELNAIRQGQT
                     VEVIFDAYPGESVSGKIAHISPILSPLTRTSTVEVVIPNNGSKLKPGMFCRASIEMPA
                     AKGIFVPINAIHRQQGTGEDFVYVVNTDNTVTRTRVTRGESQNELIRIPELDKSAVVV
                     IDGKNKLEDGAKIEIVK"
     gene            1468..4626
                     /locus_tag="PLI30_09745"
     CDS             1468..4626
                     /locus_tag="PLI30_09745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007365303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HOI79873.1"
                     /translation="MKLPEIGVKRPVATVMLFVAIIMLGLVSLKMLPLDIMPEMEFPS
                     ITIITIYPGASANEVEEQVTKPLETILSAAEHLSEIKSTSKENVSFIQLSYSWGSDVT
                     SAANNARDLIELTKTKLPQAAHQPIIYKINSSMMPVLAYAVNAGVNYNGIENIVEEDI
                     ATVLRKVDGVGTVVYLGQPEREIKVSVNPQRLSAYGLSATQISTMLKADNISVPAGNI
                     NMGVYDFSVRVPGKFETVEEIGNTVLKAFNGQVIRLKDVAKVEDTFMEKEAFARNRTG
                     EGVALMIQKQSGSNTVAVVNAVRAKMAEIQKTLPSDIQVHEIISTDEVVTQSVNNLTS
                     SIWYALVFVTLVVLMFLREWKSSLIIFITMPVSLISAFIAMYAMGYTINIFSLMALVI
                     AIGMVVDNAIVVLENITQHIEKGSKPKQAAIFGALEMGMAIAASTATTLVVFLPMLFM
                     GGIVGIMFKQLAILTSVCLIVSLFTALTLTPMLSSKLLKEAPRNGKKQHRSKFYAASE
                     KVFTKIENGYKLLLGWVVFHKSLTIVIAFAVFVLTMIVGKNIGTDYIPDFDAGTVSVV
                     FETEVGTSARQTDSISQQILQIMLEDIPEIANEAAVSIAGQTKTGALTTVGFKEGKNV
                     GTVLCHLTPPDKRDRTAAEIAGAIRDRVESIPQIEKCRVQGGSALAAAVTGNKRPIEI
                     IVSGNDFNQLNAVASDLQEKMQAEKAFTDVATTVDPGKLEVQVIVDKEKASQMALNTA
                     MVGMQVRQNLYGAESGDFSEDGNDYGIVVQYAPEHRNEVNKLKEMQVSNLLGQQIPLS
                     AIADIVESLGPLEIQRQSQQRYVKTTADLNGVSLGEATKTAQEIIDNTEIPDGISVEI
                     GGQVNDQKSSFGSLFLIFFLGIALVYMVMAAQFESMKDPFIILFAIPFTLVGVILAFF
                     VTGITLSVTTFIGLIMLVGIVVNNGIVLVDYTNMLRKRNYPLRDAVMEAGRSRLRPVL
                     MTSLTTILGMLPMAVSNGMGREMYAPLGITIIGGLLVSTLVTLLLVPAMYTSLYHRTL
                     AKDRTTARLKKQRQAENKKSLE"
BASE COUNT         1511 a         1088 c         1154 g         1089 t
ORIGIN      
        1 aagcacaatc ttaatacagc ccatataaag aagcgaaagg tgcaactcga acttgacgaa
       61 gcacgtgaaa tgatagattt acaaattcgt caaaaccggt acaagtacaa cgaatccatc
      121 agaaaagcag agcttacgca actttcgaaa gcgcaagccg aggaaaacat gaagatagcc
      181 aaagaaaacc tgctggaagg caggactcgt ctgaccgaac tgctggaagc tcaagtacag
      241 tgggaaaacg catcgtcaga atacatcgac gcattgatag aaataaaaac aacacggtcg
      301 gaacttgaaa agtcgaccgg ggaaatttac aagtatgtcg gcaattaggc aacagatatc
      361 cgatagaaag agtgaaaaaa acgataaaaa tatagactat gcaaaccaag agaactattc
      421 tgtatgcaac atgcttcgcc atactgctga tggggggatg cagcaaaaaa acaggttcca
      481 acaacgcgga aataaacgaa aaaaaagccg ttatggctac tgtaaaagaa gttgtggaaa
      541 tggaatacac ccctgtgttg aactttacag gaacggctga agcgaacaag gaagtaaatt
      601 tggggtcagc cattccggga cgcgtggaga gaattaattt tccaaaaggt agttttgtgt
      661 ccaaaggtgc cgtaatagcc gagatgtccg atgaaatgct cattcaggca caaatagaac
      721 tggaagctat tcgtaaggac ttcgaaagga tcagccgttt gaaagaaaag ggcagtgtca
      781 gcgtaatgga ctacgatcac ataaaagcca agtttgaagc ctcccaaacc aaagtggaga
      841 tgttgaagaa aaacacttcg attatcgccc ctttcggcgg ggtattggtc gatattatgg
      901 tgaatgaagg cgaaaattat tcgtttgtac cttcagtaag ccccgatttg aaagtaaaga
      961 gcggtattgc aacacttatg caattgaatc ctctgaaagt aaaaattgag ataaacgaaa
     1021 aagagcttaa cgccatccgt caagggcaga ctgttgaggt tatcttcgat gcttatcccg
     1081 gcgaaagcgt ttcgggcaag atagcccata tatcccccat actgtcgccc cttacgcgga
     1141 catcaaccgt ggaagtggtg atccccaaca acgggagcaa attgaaaccg ggcatgtttt
     1201 gccgcgcttc catcgaaatg ccggcagcca aaggcatatt cgtccccatc aacgcgatac
     1261 accgccagca aggcacaggc gaagatttcg tatatgttgt gaatacagac aacacggtta
     1321 ccagaacccg agttacgcgg ggcgaatcgc aaaatgaact tatccgcatt cctgaactcg
     1381 acaagagcgc agttgttgtc atcgatggaa aaaacaaact ggaagacgga gccaaaatag
     1441 aaatagtgaa ataaaacagc aacaacaatg aaattacctg aaataggcgt aaaaaggcca
     1501 gtggctacgg tgatgctctt tgtagccatc attatgctag ggctggtatc gctgaaaatg
     1561 cttcctttag atatcatgcc cgaaatggag ttcccctcca tcaccataat caccatttat
     1621 ccgggtgcct cggcaaacga agtggaagaa caggtcacca aaccgttgga aacgatatta
     1681 tcggcagccg aacatctcag cgaaataaaa tcgacatcta aggagaatgt gtcgttcatc
     1741 caactcagct acagttgggg cagtgatgta acttccgcag caaacaatgc ccgcgacctc
     1801 atcgagttga cgaaaacaaa attgccgcaa gcagcacatc aacccatcat ttacaaaata
     1861 aactcctcaa tgatgcccgt gctcgcctac gccgtaaatg caggtgttaa ttacaacggc
     1921 attgaaaata ttgtggaaga agacattgcc acagtacttc gtaaagtgga tggtgtcggg
     1981 accgtggtat acctcggaca acccgaacgt gaaataaaag tgagtgtgaa tccacaacgg
     2041 ttatcggctt acggtttgtc ggctactcag atctctacca tgctcaaggc agataacatc
     2101 agtgtgccgg ccggaaatat caacatgggt gtttacgatt tctcggtacg tgtgccggga
     2161 aaattcgaaa cggtcgaaga gatcgggaac accgtactga aagcattcaa cggacaggta
     2221 atccggctga aagatgtggc aaaagttgaa gacaccttca tggaaaaaga ggcttttgcc
     2281 cgcaaccgta ccggtgaagg cgtagcattg atgatacaga aacagtccgg aagcaacacg
     2341 gtagccgtgg tgaatgccgt aagggccaaa atggcagaaa tacagaaaac gctgccatcc
     2401 gatattcagg tacacgaaat aatcagcacc gacgaagtag ttacccaatc ggtcaataac
     2461 ctcacttctt cgatctggta tgccctggta tttgtcacct tggtggtatt gatgttcctg
     2521 cgcgaatgga agtcgagtct gataattttc ataaccatgc ctgtgtcgct tatcagtgcc
     2581 tttatcgcta tgtatgccat gggatacacc attaatatct tttcgttgat ggcgttggta
     2641 attgctatcg gtatggtggt ggacaatgcc attgtggtgt tggaaaacat tacccaacac
     2701 atagaaaaag gttcaaagcc caaacaggca gccatttttg gtgccctgga aatgggaatg
     2761 gctatcgccg cctctaccgc cacaacatta gtagttttcc tacccatgct ttttatgggt
     2821 ggaattgtgg gcatcatgtt caaacagttg gccatactca cttcagtttg tttgattgtt
     2881 tcgttgttta ccgctctgac actcaccccg atgctttctt caaaattgct gaaggaagca
     2941 ccccgcaacg gtaaaaaaca acatcgttcc aaattttatg cggccagcga aaaggtattc
     3001 actaaaattg aaaacgggta caagctgttg ttgggctggg ttgttttcca caaaagcctg
     3061 accatagtca ttgcctttgc cgtctttgtt cttacgatga tcgtcgggaa aaacataggc
     3121 accgattata ttcccgactt cgatgccgga acggtctctg ttgtctttga aaccgaagta
     3181 ggaaccagcg cccggcaaac cgacagcatc agtcagcaaa tcttgcaaat catgctcgaa
     3241 gatatccccg aaatagccaa tgaagctgct gtttccattg ccgggcaaac aaaaacgggc
     3301 gccctgacca ccgtaggatt taaagaaggg aaaaacgtag gtacggtact ttgccacctt
     3361 accccacccg ataagcggga ccgcacggca gccgaaatag ccggagccat cagagacagg
     3421 gtggaaagca ttcctcaaat tgaaaaatgc cgcgtgcagg gcggtagtgc attggctgct
     3481 gcggttacgg gaaacaaacg ccccatcgag attattgttt cgggaaacga ttttaaccaa
     3541 ttgaacgccg tagcatcgga ccttcaggaa aagatgcaag ctgaaaaagc atttaccgat
     3601 gtggcaacca ccgttgatcc ggggaaactg gaagtgcagg taatcgtaga caaggaaaaa
     3661 gcatcgcaaa tggccctcaa caccgctatg gtaggcatgc aggtaaggca aaacctttac
     3721 ggagccgaat cgggtgattt cagcgaagac gggaacgact acggtatcgt agtgcaatat
     3781 gcaccggaac atcggaatga agtgaacaaa ctgaaagaga tgcaggtctc caacctgttg
     3841 ggacaacaaa ttccgctgtc cgccatagcc gacatagtgg aaagcctggg accgttggaa
     3901 atacagcgtc aatcgcagca acgatatgtt aaaaccactg ccgaccttaa cggagtctcg
     3961 ctgggtgaag ctaccaagac tgcacaagag atcatcgaca atacggaaat tcccgatggt
     4021 atatcggttg aaatcggagg acaggtaaac gaccagaaat catcgttcgg ctcgctcttt
     4081 ttgatttttt tcctcggcat tgcattggtt tatatggtta tggcagccca attcgagtcg
     4141 atgaaagacc ctttcatcat tctgtttgcc attccgttta cattggtggg agtcattctg
     4201 gcgttcttcg taacgggaat aacgctgagc gttaccacgt ttatcgggct gatcatgctt
     4261 gtaggtattg tggtgaacaa cggtatcgtt ctcgtggact acaccaacat gttgcgaaag
     4321 cgtaattatc cgctccgcga tgccgtaatg gaagccggac gttcacggtt gcgccccgta
     4381 ttgatgactt cactgacaac catcctaggc atgttgccaa tggccgtaag caacggcatg
     4441 ggacgtgaaa tgtatgctcc cctcggaatc accatcatag gaggattgtt agtatccacc
     4501 ttggttacgt tactgctggt tccggccatg tatacctcgc tttatcaccg cacactggcc
     4561 aaagaccgca ctacggcaag attaaaaaaa caacgccaag cggaaaataa aaaaagcctg
     4621 gaataaacgg ggaaaggcga aagcccgatt agaaaagaac atgtttaaca aaaaacaaaa
     4681 acaacatcaa aatgaaattc atatacatta catgcaacgt aagtatgcta gagactgtca
     4741 ccagtttgct tgatgaagaa gagattactg attaccaggt aatggaacag gtaacggcaa
     4801 aaagcaacta cagcctgcca cgtttaaaca cggcagtttg gc
//
LOCUS       DAOSDR010000248         4830 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrimonas sp. isolate AMR_MDS_1826
            NODE_6224_length_4830_cov_3.349110, whole genome shotgun sequence.
ACCESSION   DAOSDR010000248 DAOSDR010000000
VERSION     DAOSDR010000248.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863355
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrimonas sp. (wastewater metagenome)
  ORGANISM  Petrimonas sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Dysgonomonadaceae; Petrimonas.
REFERENCE   1  (bases 1 to 4830)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4830)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:10:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,720
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 35
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4830
                     /organism="Petrimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6224_length_4830_cov_3.349110"
                     /isolate="AMR_MDS_1826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2023866"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1856
                     /locus_tag="PLI30_09750"
     CDS             <1..1856
                     /locus_tag="PLI30_09750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009037474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="family 78 glycoside hydrolase catalytic domain"
                     /protein_id="HOI79874.1"
                     /translation="PVIFNSIYTAEHYEARKEQPGWDTPDFDAKGWRNIYTTGAPSNN
                     IVAQALHPIRNVREYRPVSLKKIDEYTWLYNFGQNMSGVTRMKVNGPQGTTIRLKHVE
                     RLDSAGRADMSNIDVHYRPTDDSDPFQTDILILSGKKEDDFMPRFNYKGFQYVEVTSD
                     NPLDLSEENLTAYFMHSDVPPVGKINSSNNTLNKAWQASNASYLSNLFGYPTDCPQRE
                     KNGWTGDAHIAIEVGLYNFDGITVYEKWLADHRDEQQPNGVLPAIIPSSGWGYQWANG
                     PDWTSTIAIIPWNIYLFYGDTRLLELCYDNIKRYVDYIDEQYPTGITDWGLGDWVPVK
                     SVTPKEYTSSTYFYIDALILAKTAKLFGKTSDFERYSILAEKIKAAINKKYLNSETGI
                     YGSGLQTELSVALHWNLVPEELRSKVADNLARRVEQDNKHIDVGLLGTKTILNALSEN
                     GYAQLAYEVASQETFPSWGWWIVNGATTFYENWPLDAGSDISLNHIMFGEVNAWYYKA
                     LGGIFPDESQPGFKNIVLKPNFVTGLSHFEASHESPYGNVVSSWLRKGRTIEYKVTVP
                     ANSTATLYLNGKSIRENNKPLEKNPLIELNKSDQDMHILRLKAGSYSFSIK"
     gene            1881..>4830
                     /locus_tag="PLI30_09755"
     CDS             1881..>4830
                     /locus_tag="PLI30_09755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009860361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HOI79875.1"
                     /translation="MKKLYLLLILIFVCLSGCTLTHEKNNPEKFYFNEVEKNFSNPGS
                     EFRSSPLLVFNEVITKPELDRMISELHEAGFGGFFVHPRPGLVTEYLSEEWFDLFKYA
                     TDKASELGMEAWIYDENSYPSGFAGGHVPAQMPESYNQGQGFKLTKYTLLPDSLPADY
                     LCLMNSNGKYIDITSQTTDYLGKEGDYYLYAKTFYRSSPWYAGYSYVDLLLPGVTEKF
                     IDVTMEGYNRVLGSEYNKTVRGIFTDEPNIVTSGGFRWTPDLFEVFKAKWGYDLKEYL
                     PLLSEETGDWKKVRYHYMETLLQLFIDRWAKPWFEYTEEKNLIWTGHYWEHGWPNMND
                     GPDNMAMYAWHQMPGIDMLFNQFNEDSPQAQFGNVRSVKEVRSVANQMGYKRTLSETY
                     GGGGWDVTFKDLKRLGDWEYVLGINFMNQHLSHQTLTGARKYDYPPVFTYHSPWWNNY
                     KYLNDYFGRLSYVLSEGVQKNDIVIIEPNSTLWSYYSHSGGNPQYPQLMEIGKNFQSF
                     VTKLEKSQVEFDLASENIIKDNGKVQNDKFSIGKAHYTTVIIPPLTENLSKPTVELLK
                     LFVASGGKLICFSLPNRVDGEENDELTNLLNQNTVQHVTDLNPEIINSLLSGQGCKIT
                     HVGGDLYHYRKSYSDGEMLFIVNSSLNEVSTGKVSLKGASLIELDALEGNMYTYPSEK
                     EEKGLLSFEFKLEPSGSLLLFSSKKTNHNYPLRESGQQTNQVKAETETKVSRLKDNVF
                     IVDFCDLVINNKSESNLHVSKATDIVFKTNGFENGNPWNSSVQYKKNTVERDTFSGGG
                     FEVTYRFKVTGNLDKDIKLVVERPNLFSVSVNGKKASAIPGEWWLDKSFGVYSVGEFL
                     KTGENSVELSIPTMSIFAEIEPVYLLGDFTVVPEKVGWSIDAAKKELTLGSWKEQGQP
                     FYSWDVKYSKTYSIDDTTRKHTLKLGKWNGTVCEVYVNNKKAGIIGFDPYTLDVSPWL
                     EKGKNQIDVCV"
BASE COUNT         1512 a         1041 c         1083 g         1194 t
ORIGIN      
        1 gtcctgtaat ctttaacagt atatacacgg ccgaacatta cgaagcccga aaagaacagc
       61 ccggatggga cactccggat ttcgacgcga agggctggag aaacatttac accacaggag
      121 caccttccaa taatatagtg gcacaagcat tgcacccgat ccgcaatgtc agggaatacc
      181 gtccggtaag cctgaagaaa attgacgaat acacctggct ctacaatttc gggcaaaaca
      241 tgtcgggcgt aacccgaatg aaagtaaacg ggcctcaggg tacaacaatc cggctgaaac
      301 acgtggaacg acttgactcg gccggacgtg cggacatgtc aaacatcgat gtgcattaca
      361 ggcctacgga cgattcagac ccttttcaaa cggatatcct tatcctgagc ggtaaaaaag
      421 aagatgactt tatgccacgc tttaattaca agggatttca gtatgttgaa gtgacctccg
      481 ataatccgtt ggatctgtcg gaagaaaacc tgactgccta ttttatgcac agtgatgtac
      541 cgccggtagg aaaaataaat tcttccaaca acaccctcaa caaggcttgg caggcatcca
      601 acgcatctta cttatccaac ttattcggtt atcctaccga ttgtcctcaa cgggaaaaaa
      661 acggatggac tggcgacgcc catatcgcca tcgaagtagg attgtataat tttgacggga
      721 ttaccgtgta cgaaaagtgg ttggcagacc atcgcgacga acaacaaccc aacggtgtgc
      781 ttcctgccat tataccgtca agcggatggg gttaccaatg ggcaaacgga ccggactgga
      841 caagcaccat cgccatcatc ccgtggaaca tctacctttt ttacggcgat acccggttac
      901 tggaattgtg ttacgacaat attaaacggt atgtcgatta tatcgacgaa caatatccta
      961 cgggcataac cgactgggga cttggcgact gggtacccgt aaaatcggta acaccgaaag
     1021 agtatacttc aagcacctat ttttatatcg atgcgcttat ccttgccaaa acagccaagc
     1081 tgtttgggaa aacgtcagat tttgagcgct attccattct tgctgagaaa ataaaagcgg
     1141 ccatcaacaa gaaatacctt aactccgaaa ccggaattta cggaagcggt ttgcaaaccg
     1201 aattaagtgt ggccctacat tggaatttgg ttcccgaaga gctaagaagc aaagtggccg
     1261 ataacctggc acgccgtgtg gaacaagaca acaaacacat cgacgtgggg ttgttgggaa
     1321 caaaaaccat cctaaacgcg ttgagcgaaa acggatacgc acaactggcc tatgaagtgg
     1381 catcgcaaga aactttcccg tcgtggggct ggtggatagt taacggagcc accacttttt
     1441 atgaaaactg gccactcgat gcaggaagcg atatctcgct gaatcacatt atgtttgggg
     1501 aagtaaatgc ttggtattac aaagcattgg ggggaatttt cccagatgaa agccagcccg
     1561 gatttaaaaa tattgtcctt aagcccaact ttgttaccgg attatcacat tttgaagcct
     1621 ctcatgaaag cccttacggt aacgtagttt cctcatggct gagaaaaggc agaacgatag
     1681 agtataaagt gacagttcct gcaaacagta ccgcaacatt gtatttgaac ggaaagagca
     1741 ttcgggagaa taataaaccc ctcgaaaaaa atccgttgat tgaactaaac aaatcggatc
     1801 aggacatgca tattttaaga ttaaaagcag gttcctattc gttctctata aaatgatcat
     1861 ccattaaata aatttccaat atgaaaaaat tatacctgtt acttatcctc atatttgtat
     1921 gcctctccgg atgtacattg actcatgaaa aaaacaatcc tgagaaattt tactttaacg
     1981 aggttgaaaa gaatttttcc aatccgggca gtgaattcag gtcgagcccc cttctggtgt
     2041 tcaatgaagt aataaccaaa cccgaattgg atagaatgat aagcgaattg cacgaagcag
     2101 gatttggcgg tttttttgtc cacccccgtc ccggattggt tacagaatat ctttcggaag
     2161 aatggtttga ccttttcaag tacgctacag acaaagcttc ggaactggga atggaggcct
     2221 ggatttatga tgaaaactct tatcccagcg gattcgccgg aggtcacgta cccgcacaaa
     2281 tgcctgagtc ttataaccag ggacaaggat ttaaattgac aaaatacacc ctccttccgg
     2341 attctcttcc tgcagattat ttgtgtttga tgaactcaaa cggcaagtat atagatatta
     2401 cttcacaaac aactgactat ctggggaaag aaggagatta ctacctttat gccaagacct
     2461 tttaccgcag ttcgccctgg tacgccggct attcctatgt tgatttattg cttcccggag
     2521 taacggagaa attcatcgac gtgaccatgg aaggctataa cagggttttg ggaagtgaat
     2581 ataacaagac agtaagagga atcttcacgg atgaacccaa tattgtaacc tccggtggtt
     2641 tcaggtggac tcccgatctt tttgaagttt ttaaagcaaa gtggggatac gacctgaagg
     2701 agtatttacc cctgctgagt gaagaaaccg gggactggaa aaaagtccgc taccactata
     2761 tggaaacatt gctacaacta tttattgaca ggtgggcaaa accgtggttt gaatatacgg
     2821 aagaaaaaaa tctaatttgg accggacact actgggaaca cggatggccc aacatgaacg
     2881 acgggcctga caatatggcc atgtacgctt ggcatcagat gccggggatt gacatgcttt
     2941 tcaatcagtt taatgaagac tcaccacaag cccagtttgg gaatgtacga tcggtaaaag
     3001 aagttcgaag tgttgccaat caaatgggat ataaaaggac cttgagcgag acgtatggcg
     3061 gaggcggatg ggacgtgacc tttaaggact taaaacgttt gggagactgg gaatatgtat
     3121 tgggcataaa cttcatgaac caacacctat cccatcaaac cctgaccgga gcacgaaaat
     3181 acgactaccc gcctgttttc acctatcatt ctccctggtg gaataattac aagtatctga
     3241 acgattattt cggcaggtta tcgtacgtat taagcgaagg ggtccagaag aacgatattg
     3301 ttattattga acctaactcc acgttgtgga gctattactc tcacagtggt ggcaatccac
     3361 aatatccaca attgatggaa attgggaaaa attttcaatc ttttgttaca aaattggaaa
     3421 aaagtcaggt agagtttgac ctggcctccg aaaacatcat caaggataat ggaaaagttc
     3481 agaatgacaa gttttccatc ggcaaggcac actacacaac cgtaataatc cccccgctga
     3541 ccgaaaacct cagtaagccg actgttgaac tgttaaagct cttcgttgca tccggcggca
     3601 aactgatctg cttttccttg cccaacaggg tggatggcga agaaaatgat gaattgacta
     3661 atctgcttaa tcaaaacaca gtacaacatg ttaccgatct caaccccgaa atcattaaca
     3721 gtttattgtc gggacaaggg tgtaaaataa cccatgtcgg tggcgacctg tatcattacc
     3781 ggaaaagtta ttcggacggt gagatgttgt ttattgtcaa ctcctccctg aacgaagttt
     3841 caaccggaaa agtttctttg aagggagcct cactgatcga gttggatgca cttgaaggca
     3901 acatgtatac atatccttct gaaaaagaag aaaaagggct gctctctttt gagtttaaac
     3961 ttgaaccttc cggcagcttg ctcttgtttt cctcaaaaaa aacaaatcat aactatccgc
     4021 ttagggaatc cggtcaacaa acaaaccagg tgaaagccga aacggaaaca aaggtctcac
     4081 gactcaaaga caatgtcttt attgtagact tctgcgatct cgtcattaac aacaaatccg
     4141 aaagcaacct gcacgtatcc aaagcaacgg atattgtttt caaaacaaac ggttttgaaa
     4201 acggcaatcc ctggaattcg tcggtacaat acaaaaagaa cacggtcgag agagacacct
     4261 tctccggcgg tggatttgaa gttacctatc gttttaaagt tactggaaat ctggacaaag
     4321 acattaagtt agtggttgaa agacccaatc tgttttctgt cagtgtcaac gggaaaaaag
     4381 cttcggcaat acccggagaa tggtggttgg ataaatcttt cggagtatat tccgtgggtg
     4441 aatttttaaa aaccggagag aacagtgtgg agttaagcat cccaaccatg agtatctttg
     4501 ctgaaatcga acccgtttac ctgttgggag actttacggt tgttcccgaa aaagtgggct
     4561 ggagcattga tgctgccaaa aaagagttga ccctgggaag ttggaaggag cagggccaac
     4621 ctttttactc ctgggatgta aaatacagca agacctattc cattgacgat acaacaagaa
     4681 agcacaccct aaaactcgga aaatggaacg gcactgtttg cgaagtgtat gtcaacaaca
     4741 aaaaggcagg aatcattggt tttgatccct acacgctgga tgtatccccc tggctggaaa
     4801 aaggaaaaaa tcaaattgac gtatgtgtaa
//
LOCUS       DAOSDR010000249         4814 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrimonas sp. isolate AMR_MDS_1826
            NODE_6246_length_4814_cov_4.044337, whole genome shotgun sequence.
ACCESSION   DAOSDR010000249 DAOSDR010000000
VERSION     DAOSDR010000249.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863355
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrimonas sp. (wastewater metagenome)
  ORGANISM  Petrimonas sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Dysgonomonadaceae; Petrimonas.
REFERENCE   1  (bases 1 to 4814)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4814)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:10:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,720
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 35
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4814
                     /organism="Petrimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6246_length_4814_cov_4.044337"
                     /isolate="AMR_MDS_1826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2023866"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            43..657
                     /locus_tag="PLI30_09760"
     CDS             43..657
                     /locus_tag="PLI30_09760"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019538703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOI79876.1"
                     /translation="MDVEIYIATSAYAKYAQEVCDLIYESAQARGTGIAKRSAEYVAE
                     KINAGKAVIALDGDKLVGFAYIETFSHQRFVANSGLVVHPDYRNMGLARKIKRKIFDL
                     SRKLFPHAKIFSITTGLAVMKMNTELGFKPVTFSELTDDEEFWKGCQGCRNFDILQRN
                     NYKMCLCTGLLYDPAQKEVAASSFAKKVVKPIIKKKAPNKMYKK"
     gene            741..1925
                     /gene="argG"
                     /locus_tag="PLI30_09765"
     CDS             741..1925
                     /gene="argG"
                     /locus_tag="PLI30_09765"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HOI79877.1"
                     /translation="MKQKVVLAFSGGLDTSFCVPYLINKRNLEVHTVIVNTGGFSDQE
                     LKGIEERSKILGALSHTTIDAVNDYYEKGIRYLIFGNILKNNTYPLSVSSERFFQALS
                     VLEHVKKIGAEYVAHGSTGAGNDQVRFDLVFEVLAPDVKIIAPIRELGLSRQEEIDYL
                     KSNGFNFSWEKSKYSINQGLWGTSVGGVETLKSSGILPEEAYPSQVSDHGVERIKIGF
                     EKGEPVSLNGEKMSPVNVIRTLHKWASRFGIGRDVHVGDTIIGTKGRVAFEAPAPLIL
                     VKAHHLLEKHVLTKQQLYWKDQLSNWYGQLMHEAQYLEPVMRNIETFLDDTQQFVTGE
                     VDVELRPYHFSVLACSTDYDLMNPVFGEYGEVNKSFTGQDVIGFTKVISNPLKIYYTI
                     HK"
     gene            1940..2914
                     /gene="argC"
                     /locus_tag="PLI30_09770"
     CDS             1940..2914
                     /gene="argC"
                     /locus_tag="PLI30_09770"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015547033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HOI79878.1"
                     /translation="MIKVSIAGGTGYTAGELLRILLNHPGVTLESVISSSSAGMPVAG
                     IHRDLLGETDITFSDNTGHPDVIFLCLGHGLSRAFLNENDIPAACKVIDLGNDFRNDP
                     VFGERNFVFGLCELNREQIRKANSVANPGCFSSSIMLALLPLAALNKLTDDIHVHAIT
                     GSTGAGKKPVGTTHFSYRDNNISVYKPFTHQHLAEIERTLKAAGNKVLPPIQWIPMRG
                     DFTRGIFASVYTRWDNLMSETEVINYYKEYYKTSPFVFVSDEPISLKEVVNTNKCLLH
                     IGFHNGYIHITSVLDNLLKGASGQAVQNMNLMFGLDETMGLRLKGSVF"
     gene            3005..4132
                     /locus_tag="PLI30_09775"
     CDS             3005..4132
                     /locus_tag="PLI30_09775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HOI79879.1"
                     /translation="MNLFDVYSLWNIEPVRAKGCRVWDRDGVEYLDFYGGHAVISIGH
                     SHPVYVKAIQDQAVKIGFYSNSVINSLQTELAEKLGEQSGYPDYSLFLCNSGAEANEN
                     ALKLASFHTGKKRIIAFSGSFHGRTSGAVAITDNPSIQSPFNTGHEVTFVPLNDTDKI
                     LRELEKGDVAAVIAEGIQGVAGIFVPENGFMQQLEKACHKHRVVLILDEIQSGYGRTG
                     RFFAHQHAGIRPDIITLAKGMGNGFPIGGMLISPEFKAKKGMLGTTFGGNHLACAAAI
                     AVLDVMKGEALVENASQAGNYLQDELSKLNGIKEIRGRGLMLGIDPAPEYSDIRDKLL
                     FKSHIFTGGAKNNVMRLLPPLSVKKEEIDIFLKETKKLLEV"
     gene            4150..>4814
                     /locus_tag="PLI30_09780"
     CDS             4150..>4814
                     /locus_tag="PLI30_09780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005784352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylornithine carbamoyltransferase"
                     /protein_id="HOI79880.1"
                     /translation="MKHFTCVQDIGDLSSALQKAKYTKENPFSDQHLGKNKTLMLIFF
                     NSSLRTRLSTQKAGINLGMNVIVLDINQGAWKLETERGVIMDGDKPEHILEAIPVMGA
                     YCDIIGVRSFAQFENKSDDYNELIINQFIQYSGRPVFSMEAATRHPLQSFADLITIEE
                     FKKTARPKVVLTWAPHPKALPQAVPNSFAEWMNATDYEFVITHPEGYELDDKFVGKAI
                     VEY"
BASE COUNT         1382 a         1065 c         1150 g         1217 t
ORIGIN      
        1 tccttctttt atttatcatt tataaaaaat tttattacac aaatggatgt ggaaatttat
       61 atagctactt ccgcttacgc aaaatacgca caggaagtgt gtgatttaat atacgaatcg
      121 gctcaggcac gcggtacagg tattgccaaa cggagcgcag agtacgtcgc ggaaaaaata
      181 aatgccggca aggcggtcat agcgctggat ggggataagc tagtgggatt cgcctatatt
      241 gagacgttca gccaccagcg gtttgttgcc aactcgggac ttgttgtgca tcccgattat
      301 cggaacatgg ggttagcccg gaaaataaaa cgaaaaattt ttgatctttc caggaagcta
      361 tttccacacg ctaaaatatt cagcattact accggactgg cagtaatgaa gatgaacact
      421 gaactgggct ttaagccggt aaccttttcc gagcttaccg acgatgaaga attctggaaa
      481 ggttgtcagg gttgccggaa tttcgatatt cttcaacgca acaactacaa aatgtgcctt
      541 tgcacagggc tattgtacga tccggcacag aaagaagttg cagccagttc gtttgcaaaa
      601 aaagtagtga aaccgatcat aaagaagaaa gctccgaata aaatgtataa aaaataataa
      661 tcacggttat ttgctgttcg cctctccggt tcaatgcagg gccaatagcc aacaacaggt
      721 aaccaaaaag cctaaaatat atgaaacaaa aagtagtttt ggcattcagc ggcggtctcg
      781 acacttcatt ttgtgtgccg tatcttatca acaaacgcaa tttggaggta catacagtaa
      841 ttgtcaacac tggcggtttt tcagaccaag agctgaaagg cattgaagag cgttccaaga
      901 tcctgggagc gttatcccat acaacaattg atgccgtaaa cgattattac gaaaaaggca
      961 tcaggtatct gattttcggc aatattctta aaaacaacac ctaccctctt tctgtcagtt
     1021 ccgaacgttt cttccaggcc ctatcggtgc tggaacacgt aaaaaaaatc ggtgccgaat
     1081 atgttgccca cggaagtacc ggtgccggga atgatcaggt acgcttcgac ctggtttttg
     1141 aagtgttggc tcccgatgta aagattatcg ctcctatccg ggagttaggg ttatcgcgtc
     1201 aggaagaaat tgattacctg aaatcgaacg gatttaattt ttcttgggaa aaatcgaaat
     1261 attccatcaa ccaggggttg tggggaacaa gtgttggtgg cgttgaaacg ttgaagtcat
     1321 caggcattct tcccgaagaa gcctacccct cacaagtctc tgatcacggc gtggagcgaa
     1381 taaagattgg ttttgaaaaa ggagaaccgg tttccctgaa cggtgagaaa atgtcacccg
     1441 taaacgttat ccgcacactg cacaaatggg catcccgatt cggtattggc agggacgttc
     1501 atgtaggcga caccattatc ggaacaaagg gaagggttgc cttcgaagct ccggcacccc
     1561 tgattctggt aaaagcacac cacttgttgg aaaagcacgt gcttaccaag caacaactct
     1621 attggaaaga tcagctatcg aactggtacg ggcagttgat gcatgaagcc cagtacctgg
     1681 aacccgttat gcgcaatatt gaaacgtttt tggatgacac ccagcagttt gttacgggtg
     1741 aagtggatgt agagcttcgt ccttaccatt tctcggtatt ggcttgcagc acggattacg
     1801 atttgatgaa tccggttttc ggagagtatg gtgaagtgaa caaatcgttt accggacaag
     1861 acgtcattgg ttttaccaag gtaatttcga atccgttgaa gatatattat acaattcata
     1921 aatagtctta atcgataaaa tgattaaagt atccattgcc ggagggaccg gctacactgc
     1981 cggcgaactg ctccgtatcc tgctgaatca tcccggggtg acgttagagt cggtaatcag
     2041 ttcgagctcc gccggaatgc cggttgccgg tatacaccgt gacttattgg gcgaaaccga
     2101 cataacgttt tccgacaata ccggacatcc ggatgtcatt tttctttgcc tcggccacgg
     2161 actttcacgt gcctttctta acgagaatga tatccctgcg gcttgcaagg taattgactt
     2221 gggaaatgat tttcgcaacg atccggtgtt tggtgagcgg aattttgttt tcgggttatg
     2281 cgaactcaat cgggaacaaa tccgcaaagc aaacagcgtg gccaatccgg ggtgtttttc
     2341 ctcgtcaatc atgttggctt tgctgccgct ggcagcgttg aataagttga cggacgacat
     2401 tcacgtacat gccatcaccg gatcgaccgg tgccggaaaa aagcctgtgg gcaccacgca
     2461 tttcagttat cgggacaaca atatctccgt ttacaaacct tttacccacc agcatttagc
     2521 ggaaattgaa cgaacgttaa aagctgccgg aaacaaggtg ctgccaccca tacagtggat
     2581 tcccatgcgc ggtgatttta cccgggggat tttcgccagt gtgtacaccc ggtgggataa
     2641 cctgatgtcc gaaaccgaag tgatcaatta ctacaaggag tattacaaga cctcgccttt
     2701 tgttttcgtt tcggatgagc caatcagcct gaaagaagtg gtaaacacca ataaatgcct
     2761 gcttcacatc ggatttcaca acggatatat tcacataact tctgttcttg acaacctgtt
     2821 gaagggagca tcaggacaag cagtacagaa tatgaacctg atgttcgggc tggatgagac
     2881 catgggatta agattaaaag gaagcgtatt ttgattcgct ctgccgactg aagccagact
     2941 ttaagatttt tgtatgtctg gtgtctgaag tctgctatca aataattaat aattcacaat
     3001 taaaatgaat ctgtttgacg tatattcttt gtggaatatt gaacctgtcc gcgcaaaggg
     3061 ttgccgcgta tgggacaggg acggtgtgga atacctcgat ttttacgggg ggcacgccgt
     3121 gatttccatc ggccattccc atcctgttta cgtaaaagcc attcaggacc aggcagtaaa
     3181 aatcgggttt tattccaact ccgttatcaa cagcctgcaa acagagcttg ccgaaaagct
     3241 gggggagcaa agcggttatc ccgattattc gctttttctt tgcaacagtg gggccgaagc
     3301 caatgaaaac gcattaaaac tggcctcttt ccacacgggg aaaaagcgaa taatcgcgtt
     3361 cagtggatcg ttccacggac gcacatcggg tgctgttgcc ataaccgaca acccgtcgat
     3421 ccaatcgcct ttcaacaccg ggcacgaagt aacattcgtc cccctgaacg ataccgacaa
     3481 gatactgcgt gaactggaaa aaggcgatgt cgcagccgtt atcgcggaag gaattcaggg
     3541 cgtagccgga attttcgttc ccgagaacgg atttatgcaa cagcttgaaa aggcctgcca
     3601 taaacatcgt gtggttctta ttctcgatga aattcaatcg ggatacggcc gtacaggacg
     3661 tttttttgcg catcagcatg ccggtatccg acccgacatc atcaccctgg cgaaaggaat
     3721 gggtaacggc tttccgatcg gaggcatgtt gatttcacct gaatttaaag caaaaaaagg
     3781 aatgttggga accaccttcg gagggaatca cctggcctgt gctgcggcaa tagccgtcct
     3841 ggatgtgatg aaaggagagg cgttggttga aaatgcatcc caggcaggta actatttgca
     3901 agacgaacta tcgaaattaa acggaattaa agaaattcgc ggccgtggat tgatgctggg
     3961 aattgatcca gcccccgaat actccgatat acgcgacaag ttattgttta aaagtcatat
     4021 ctttacaggc ggagcgaaaa ataacgtaat gcgtttacta cccccgcttt cggtaaagaa
     4081 agaagaaatc gatatttttt tgaaagaaac aaaaaagctc ttggaagttt aacgttaaaa
     4141 gtctatggta tgaaacattt cacctgcgtt caagacatcg gcgacctatc gtccgccctg
     4201 caaaaagcca agtacaccaa ggagaatccg ttttccgacc agcatctcgg aaaaaacaaa
     4261 accctgatgc ttatcttttt taattcgagt ttacgcacca ggttaagcac acaaaaagcc
     4321 gggataaacc tgggtatgaa cgttattgtg cttgatatca accagggggc atggaagctc
     4381 gaaacagagc gtggagtaat aatggatggc gacaagccgg aacacatcct cgaagctatt
     4441 cctgtgatgg gtgcgtactg cgacattatc ggtgtgcgtt cgtttgcaca atttgaaaac
     4501 aaatcagatg attacaacga attgattatc aatcaattta tccaatattc cgggaggccg
     4561 gttttcagta tggaagctgc cacgcgacac ccgcttcaaa gttttgccga ccttatcacc
     4621 attgaagaat tcaaaaaaac ggcccgaccg aaggttgtcc tcacctgggc accgcatccg
     4681 aaggcgctgc cccaggcagt ccccaactcg tttgccgaat ggatgaacgc aacggattac
     4741 gaatttgtaa tcactcatcc ggaaggatat gagctggacg ataagtttgt aggcaaagcc
     4801 atcgtggaat acga
//
LOCUS       DAOSDR010000250         4806 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrimonas sp. isolate AMR_MDS_1826
            NODE_6259_length_4806_cov_3.435908, whole genome shotgun sequence.
ACCESSION   DAOSDR010000250 DAOSDR010000000
VERSION     DAOSDR010000250.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863355
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrimonas sp. (wastewater metagenome)
  ORGANISM  Petrimonas sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Dysgonomonadaceae; Petrimonas.
REFERENCE   1  (bases 1 to 4806)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4806)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:10:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,720
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 35
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4806
                     /organism="Petrimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6259_length_4806_cov_3.435908"
                     /isolate="AMR_MDS_1826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2023866"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(350..3199)
                     /gene="gcvP"
                     /locus_tag="PLI30_09785"
     CDS             complement(350..3199)
                     /gene="gcvP"
                     /locus_tag="PLI30_09785"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006843821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase"
                     /protein_id="HOI79881.1"
                     /translation="MEMEHFKTRHIGIKPEDLGNMLQTVGVSSVDELINQTIPADIRL
                     KKPLSLPKAQSEMEYAEEIGRIASANRVFTSYIGMGWYDTITPAAIYRNVFENPVWYT
                     SYTPYQAEISQGRLEALLNFQTAVSELTGLPLANCSLLDEATAAAEAATMMYGLRSRD
                     QVKNNTEILFVDQYIFPQTLGVLRTRMGMAGIEIEVGDYKCFDAEKKYFGAIVQYPNS
                     NGNVEDYRSFVEKAHASDCKVAVAADLMSLVLLTPPGEWGADIAFGSSQRFGIPMFFG
                     GPSAAFFATRDEYKRSMPGRIIGISKDVYGKDALRMALQTREQHIKREKATSNICTAQ
                     ALLATMASFYAVYHGPEGLKKIAKRIHSVTSLVADELKELGFKQLNSSYFDTLKLQLP
                     ENITQGGVKDLAEMRNINLRYFDTGEIGISIDETTDGYRVHELLAVFAMAAGSSKVFM
                     IDEMPEKITLKGDQLRTSDFLSHPNFNNYHSETELMRYIKRLERKDISLAHSMISLGS
                     CTMKLNAASELLPLGHPGFQKIHPFAPEDQVQGYQEMIHELESMLAEITGFTATSLQP
                     NSGAAGEFSGLLVIASYLKSIGQGHRKTVLIPASAHGTNPASAVQAGFNPVTVATDMK
                     GNVDMEDLRKKVETHRDDLAAYMITYPSTHGIFEVEIREMCNIIHEAGGQVYMDGANM
                     NAQVGLTNPGTIGADVCHLNLHKTFAIPHGGGGPGVGPICVAEHLVPFLPGHPVMFES
                     GVNAVSAAPYGSAGVLAITYGYIKMMGTDGLTEATKIAILNANYLAEKLNDSYGIVYR
                     GANGRVGHELILECRGLKSVSGITETDIAKRLMDYGFHAPTLSFPVHGTLMIEPTESE
                     SLAELNRFVDAMQQIHAEIIEVSRGEYTAEDNVLVNAPHPEYESVADEWKHAYPRSKA
                     VYPLPFVAENKFWVNVARVDNAFGDRNLVACLCIM"
     gene            complement(3289..3939)
                     /locus_tag="PLI30_09790"
     CDS             complement(3289..3939)
                     /locus_tag="PLI30_09790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019540864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOI79882.1"
                     /translation="MKIKTFEFNPLGVNTYVLYDRTGECIVVDASCFFPDENKLLLNF
                     ILDNNLVVKHLVNTHLHFDHIFGVNFLASQFGLKLLVHRDDIVLLENLSQQLQLFGFN
                     TNVDYRPEIGGFLAENDVVSFGEQQLKVLHVPGHSPGSIVFYNEKEKLALVGDVLFNG
                     SIGRTDLLEGSFEQLTEGIRTKLFTFSDDTEVYPGHGPKTTIGQERRYNPFVGTGS"
     gene            complement(4092..>4806)
                     /locus_tag="PLI30_09795"
     CDS             complement(4092..>4806)
                     /locus_tag="PLI30_09795"
                     /inference="COORDINATES: protein motif:HMM:NF027708.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Sb-PDE family phosphodiesterase"
                     /protein_id="HOI79883.1"
                     /translation="GDTDDYLTVNTNETDREAVMKAEAQNAFIFWNHPGWQPGIAGSY
                     EWLPFIEDLYKNKALDGIEVVNGTGFHMKALDWCIDKNLTVMGSTDVHNLISLSYDNS
                     RDYVNRTMTLVLVRDNTPESVREALDARRTVAWASKYLMGKEENVRSLFNACVEVLPS
                     HHSETNREGKTIKYYQIRNNSDLYFEMERTAGNGPGRIVLYPQSSQVIAAESGQPVYS
                     VVTTYVRSDKHLSVNLLLP"
BASE COUNT         1248 a         1161 c         1037 g         1360 t
ORIGIN      
        1 atatcgtact ggagatattt ggatatctca acgtaaattt ctaacagcaa gtgatagggc
       61 tgttgtagct ttgaagtatg aatatcctta tgacgcagca actgacactt ctttaacgca
      121 tgaaataatt tatggccctc ccactcctta ggaacagtaa tattttagaa atttgaaaaa
      181 ttccgtcaac agataatgaa taaccttatg aaaattaaat tattaaccac tttcacatat
      241 tgatatcagc aaccattatt cggtgtaaga ctttcatccg tcggaaaatt tattaataca
      301 ctactgccta cttattaaaa taggcagtag tgtatagtat atcattatgc tacataatgc
      361 ataaacacgc taccaggttt ctgtcgccaa acgcattatc cacgcgggca acatttaccc
      421 agaatttgtt ttctgccacg aaaggcaacg gataaaccgc cttgctgcgc gggtaagcat
      481 gcttccactc gtcggcaacc gattcgtact cgggatgcgg agcatttacc agtacattgt
      541 cttcggcagt gtattcgcct cgtgagacct cgatgatttc ggcatggatt tgctgcatgg
      601 catccacaaa tcgattcaac tcggcaaggc tttcactttc ggtaggttcg atcattaacg
      661 tcccgtggac agggaacgag agtgtgggcg catgaaatcc gtaatccatc agtcgtttgg
      721 caatatccgt ttctgtgatt ccggaaacgg atttcaatcc ccggcattcc agaatcaact
      781 cgtgccctac cctgccgttc gctccgcgat aaacgatccc gtaagaatcg tttaatttct
      841 cagccagata gttggcgttc aagatagcta ttttagtggc ttccgttaag ccatccgttc
      901 ccatcatttt tatatatccg taagtaatag caagcacgcc tgcgctgccg taaggagcag
      961 cagagacggc attcacgccc gattcgaaca tcactggatg gcccgggagg aagggcacca
     1021 aatgttcggc cacgcaaata gggccaactc ccggcccacc accgccgtga gggatagcaa
     1081 atgttttgtg caggttgagg tggcaaacat cggctccgat agttccgggg ttagttagcc
     1141 cgacctgtgc gttcatgttc gcaccgtcca tataaacctg tccgcccgcc tcgtgaatga
     1201 tgttgcacat ttcccggatc tcgacttcga agataccgtg tgtagaggga taggtaatca
     1261 tgtaggcagc gagatcgtca cgatgtgttt ctactttttt ccgcaaatct tccatatcga
     1321 cgttcccttt catatccgtc gccacagtca ccgggttaaa accggcctgc actgcgctgg
     1381 caggattggt tccgtgtgca gaagccggga taagcactgt cttgcgatgt ccctggccaa
     1441 tgctcttcag gtaactggct ataaccagta acccggaaaa ttctccggca gcacccgaat
     1501 tgggttgtaa gctcgttgcc gtaaatccgg taatttcagc caacatggat tccaattcat
     1561 gaatcatttc ctgataaccc tgcacctggt cttcaggagc aaatgggtgt attttctgaa
     1621 atcccggatg ccccagaggg agaagctctg atgccgcatt cagtttcatg gtgcatgacc
     1681 cgagcgaaat catggaatgt gccagggata tatcttttct ttccaaccgc ttgatgtaac
     1741 gcatcaactc agtctcggag tgataattat tgaagttggg atgagacagg aaatccgagg
     1801 tgcgcaactg atctcccttc aacgttattt tctccggcat ttcgtcaatc ataaatacct
     1861 tggagcttcc tgccgccatg gcaaacacag ccagtaattc atgaacacga taaccgtctg
     1921 ttgtttcatc gatactgata cctatttctc cggtatcgaa gtaacggaga ttgatgttgc
     1981 gcatttcagc caggtctttc acaccgcctt gcgttatatt ttcgggaagc tgcagtttta
     2041 gcgtatcgaa gtagctacta ttaagctgtt taaacccgag ttcttttaac tcatctgcca
     2101 ccagcgaggt aacggaatga atacgtttcg caatcttttt caatccctcg ggtccgtggt
     2161 aaacggcata gaacgaggcc atagttgcca gcaatgcctg agcagtacaa atattggacg
     2221 tggccttttc gcgcttgatg tgctgttccc gtgtttgcag ggccatgcgc agtgcatcct
     2281 tgccgtaaac gtctttcgat atgccaatga tcctccccgg catactgcgt ttatactcat
     2341 cacgcgtagc aaaaaaagca gccgaaggac caccgaaaaa catgggaatc ccaaaacgct
     2401 ggctgctgcc gaaagcaata tcagctcccc actcgcccgg aggtgtcaac aatacgagcg
     2461 acatcaggtc cgcggcaaca gccactttgc aatccgatgc gtgtgctttt tccacaaaac
     2521 tccggtaatc ttccacattt ccgttgctgt tgggatactg tacgatggcg ccaaaatatt
     2581 ttttctccgc atcgaaacat ttgtaatccc ccacttcaat ttcgatgcct gccattccca
     2641 ttcgcgtgcg taagacgccc agtgtttgcg ggaaaatgta ttgatcaaca aaaaggattt
     2701 ccgtgttatt tttcacctga tcgcggcttc gcaacccgta catcattgtt gctgcttctg
     2761 cagctgcagt agcttcgtcc agcagcgaac aattggccag gggcaggccg gtcagttcgc
     2821 tcactgccgt ttgaaaatta agcaatgcct cgagccttcc ctgcgagatc tcggcctggt
     2881 atggcgtata agaggtgtac catacgggat tttcaaatac gttgcggtaa atggcagcag
     2941 gcgtaatcgt atcgtaccat cccataccga tgtatgacgt aaaaactctg tttgccgacg
     3001 caatacgtcc gatctcttcg gcgtactcca tttcggattg tgctttaggc aatgataaag
     3061 gcttctttag ccggatatct gccggaatag tttgattgat cagttcgtca acagaagaga
     3121 ctcctacggt ctgcaacatg ttgcccaagt cctccggctt aattccgata tgacgggttt
     3181 taaaatgctc catttccata tagttatttg tttaaagttt aattatgatt tacaaattac
     3241 gaattaagag catcttagct ccttatcctt tgtttttcac ccgtggtcct aactccctgt
     3301 tccaacaaaa gggttgtatc ttctctcctg tccgatagtt gttttggggc catgtcccgg
     3361 gtaaacttcc gtatcatcgg aaaaagtaaa gagttttgtc cgaatacctt cggtaagttg
     3421 ttcaaagctt ccttcgagca agtctgtgcg tccgatgctt ccgttgaaaa gtacatctcc
     3481 gaccagggca agcttctctt tttcgttgta gaaaacaatg cttccgggag aatgtccggg
     3541 gacgtgcaag accttcaatt gttgctctcc aaaagaaact acatcgtttt cagcaagaaa
     3601 accgccgatt tcaggccggt aatccacatt tgtattgaat ccaaacagct gaagttgttg
     3661 cgacaggttt tcaagcaaga caatatcgtc ccgatgcacc aggagtttca aaccaaattg
     3721 cgatgctaaa aaatttaccc cgaaaatatg atcaaaatgc aaatgagtgt ttacaagatg
     3781 tttaacaact aagttgttat ccagtataaa attaagcagc agcttattct catcggggaa
     3841 aaaacaggat gcatccacaa caatacactc acccgttctg tcatataata cgtaggtgtt
     3901 aactcccaat gggttaaatt cgaatgtctt aatcttcatc tatcggcaga aatttcttgt
     3961 aatgcgtttc ctattttgat acaaatatac ggctttcttt tcaaattgta gtgtaaaaaa
     4021 aagctattcc ggcgtcatct cgaaaatgaa aaccggaata gcatatttcg ttttatatct
     4081 ccctaaaaaa attaaggtaa cagtaaattc acagaaagat gtttgtcgct gcggacgtaa
     4141 gtggtaacca cgctgtatac gggttggccg ctctcggcgg caattacttg tgacgattgc
     4201 ggataaagga caatacgtcc ggggccgtta ccggctgtac gttccatctc gaaatacaag
     4261 tcgctgttgt ttctgatctg ataatatttg atggtttttc cctcgcgatt ggtttcggaa
     4321 tgatggctag gaagaacttc cacacatgca ttaaacagag agcggacatt ttcttctttt
     4381 cccatgaggt atttacttgc ccatgccact gttcttcgtg catccaacgc ttcccggaca
     4441 gattcgggag tgttatcacg gaccagaaca agggtcatcg tacggtttac ataatctcgg
     4501 ctgttgtcat aactaaggct gattaaattg tgaacatctg tcgatcccat tacggtcagg
     4561 tttttgtcga tacaccaatc cagtgctttc atgtgaaaac ctgttccgtt aacaacctct
     4621 attccgtcga gcgctttatt tttatacaaa tcttcaataa acggaagcca ttcatacgaa
     4681 ccggctattc cgggctgcca accgggatgg ttccagaaaa taaaagcatt ttgtgcctcg
     4741 gctttcatca cggcttcacg atccgtctcg ttggtgttca cggttaaata atcgtccgta
     4801 tcaccg
//
LOCUS       DAOSDR010000251         4735 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrimonas sp. isolate AMR_MDS_1826
            NODE_6375_length_4735_cov_4.586111, whole genome shotgun sequence.
ACCESSION   DAOSDR010000251 DAOSDR010000000
VERSION     DAOSDR010000251.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863355
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrimonas sp. (wastewater metagenome)
  ORGANISM  Petrimonas sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Dysgonomonadaceae; Petrimonas.
REFERENCE   1  (bases 1 to 4735)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4735)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:10:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,720
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 35
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4735
                     /organism="Petrimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6375_length_4735_cov_4.586111"
                     /isolate="AMR_MDS_1826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2023866"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            286..1623
                     /locus_tag="PLI30_09800"
     CDS             286..1623
                     /locus_tag="PLI30_09800"
                     /inference="COORDINATES: protein motif:HMM:NF017727.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NnrS family protein"
                     /protein_id="HOI79884.1"
                     /translation="MYSTMIDNQLYRYFFISALVTIFTLGCMWGAVNLLLIGLGESFH
                     SIDYSWVLAHGHAMIFGFVGLFIMGFSFQAMPRFAGVTLRNPRLAFSTLPLLITGILL
                     QALAHVMAPQPPYLPLGIFAGLLQLIAVAIFVWVMVRTLRGAPTKGYYNGFVYAALGW
                     FLVAAVANPIIFSLFEAAATPEEFLFNVSSFNIPYRDIQTLGIAVVMILGVSLHILPH
                     AYGFRTPSRRWRRFLLWGVNGAVLFGIISFTAGMTTGVHWWHAANGISYIIFLVAAIG
                     TPIQFRLFRPLPAGSSDRSLKFIRAAFLWFIVATLLLLFGPYYMFGIYLPMTGGENPF
                     SHAYFGAYRHALTVGFIMMMIVGVSAKVVPGFSGIDIRKTSSLWLVFILLNVGNTWRI
                     VSQITTDFYPEAFRFIGISGFIELTALGLWGYELIRNIRAGIRLKHSPQQQDC"
     gene            complement(1630..2727)
                     /gene="pdxA"
                     /locus_tag="PLI30_09805"
     CDS             complement(1630..2727)
                     /gene="pdxA"
                     /locus_tag="PLI30_09805"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005646528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050570 - 4-hydroxythreonine-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HOI79885.1"
                     /translation="MSEQIKVGITHGDINGVGYEILLKTFADERMQELFIPVIYGSSK
                     SASYHRKVLDHSPVSFHIINHVDECSPGKINLLNCVKEEVRIELGTATAEAGESAFIA
                     LDNAARDLAAKKIDVLVTLPINKDTIQNDKFHFPGHTEFLQDRFAENNKALMILASNS
                     LRIALVTTHIPLAEVSEKISKELITEKLSTLNFSLKRDFRIETPRIAVLGLNPHAGEN
                     GLLGQEELRIIAPAVKEAQDAGILCAGPFAADGFFGTGRYREFDAVLAMYHDQGLVPF
                     KTIAMDSGVNFTAGLPIVRTSPDHGTAYDIAGQNKASEESFRQAIYMAIDIFNNRRDY
                     DEAGENPLRKMFFDRGKDDVRLDLTEEEEEA"
     gene            complement(2765..3751)
                     /locus_tag="PLI30_09810"
     CDS             complement(2765..3751)
                     /locus_tag="PLI30_09810"
                     /inference="COORDINATES: protein motif:HMM:NF027451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4837 family protein"
                     /protein_id="HOI79886.1"
                     /translation="MRRIHLLLLPALAILLLSSCDGSGFLSASAMSSEVLVIMDEDEW
                     EGETGRALFDVLNSPAKGLPQFEPNFRVIQLTPENFTSTFKVARNIIMPEISSIYSVA
                     KLSSELDKYASGQVIMTVRAPDTAAFINFLKENKEGIVNYILNKEMERTAQWLIKDSG
                     TPQSHIKQVFGFNIYYPKGLSNITEHPNFYWATNSAGRARKDIVIYQFPYTSESVFEK
                     DSLIGIRNRVLGQYITGSFDSHMTTATQAYNPDYRKMEYNGIFRAELRGLWEMTSDMM
                     GGPFVSHAFVNEETNMVVVVEVFVFAPEADKRNLIRSMEGALYTISFPKAKK"
     gene            complement(3887..>4735)
                     /gene="rlmN"
                     /locus_tag="PLI30_09815"
     CDS             complement(3887..>4735)
                     /gene="rlmN"
                     /locus_tag="PLI30_09815"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007765286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HOI79887.1"
                     /translation="GRVEPVDVKTSVDGTQKMLFPVKNGKFIESVNIPEEERLTICVS
                     SQVGCKMDCSFCMTGKMGFLGSLSVNEILNQVYSVPNAEALTNLVFMGMGEPLNNFDN
                     VMKAIEVLTADYALAWSPKRITLSTIGVIPSLKRFLEESRCHLAVSLHSPLSVQREKL
                     MPIEKTFKAADIIELLREYDWSHQRRLSFEYIMFDGVNDAPVYARELSKFLSGLDCRV
                     NLIRFHKIPGSDLSPSTEETMIAFRDFLTAKGFTCTIRASRGEDIFAACGMLSTTKEP
                     RTGIQD"
BASE COUNT         1055 a         1168 c         1152 g         1360 t
ORIGIN      
        1 cctgtcccgg cagggtcaaa gtaataccgc agattatcag taatatggaa agaatttttt
       61 tcatttctta caaagaatcg gcgcacctgc aaagatactg attttctgga agtacgtttt
      121 ttttttcact aaaaactaca ggcctgtaaa aatataggaa ggtttttatt cgcgaagaat
      181 aaaaaattct tatacatttg tcgtatataa tcacagctgt ctgcctctgt tgggatttct
      241 gttcattgaa ccggagatct caattgtatc ccggacaata aattaatgta tagtacaatg
      301 atcgacaatc aattataccg ttatttcttc ataagcgcct tagttacgat attcacattg
      361 ggttgtatgt ggggagccgt caatctgctt ctgatcgggc ttggggaaag cttccacagt
      421 atcgactact cctgggtact ggctcacggc catgcaatga tctttggctt cgtggggctt
      481 tttattatgg ggttttcctt tcaggcaatg ccccggtttg cgggagtgac gcttcggaac
      541 ccccgtttag ccttttcgac gttaccgctc ctgattacgg gtatcctgct gcaggcgctg
      601 gcccatgtaa tggctcccca accgccgtac cttccgctcg gcattttcgc cgggttgctg
      661 caacttatcg cagtggccat ttttgtgtgg gttatggttc gtactcttcg tggcgcaccg
      721 accaaaggat attacaacgg ctttgtctat gcggcgcttg ggtggttcct cgttgctgct
      781 gtcgccaacc cgattatatt cagtttgttt gaagcggcag caaccccgga agaatttctc
      841 ttcaacgtct cctcgttcaa tataccctat cgtgacattc aaacattagg gatcgccgta
      901 gtgatgattc tgggggtgtc cctgcatatt ctcccgcatg cctatggatt tcgtacacct
      961 tcaaggcgct ggagaagatt tttattgtgg ggcgtgaacg gggccgttct ttttggcatc
     1021 atctcgttca cggccggcat gactaccggt gtgcactggt ggcacgccgc aaacggtatc
     1081 tcctacatca tattcctggt ggcagccatc ggtacaccca ttcagttccg cttgttcagg
     1141 ccgctgccgg caggcagtag cgaccgaagc ctgaaattta tccgggcagc ctttctctgg
     1201 tttattgtag ctacgctgtt gctgctgttt gggccctatt acatgttcgg catctatctt
     1261 cccatgacgg gaggggagaa tccattctcg cacgcttact tcggtgccta ccgccatgca
     1321 ttgacagtcg gctttatcat gatgatgatt gtcggtgttt ccgctaaggt cgtgcccggt
     1381 ttttcgggta tcgacattcg gaagacaagc tcgctttggc tggttttcat cctgctcaat
     1441 gtgggcaaca cctggcgtat tgtctcgcag attacgactg atttttaccc cgaagctttt
     1501 cggtttatcg gcatctccgg ttttattgag ctcacggcgc tgggcttatg ggggtacgaa
     1561 ctgatccgga atatccgagc aggtattcgt cttaaacact caccacagca gcaggactgc
     1621 tgattcgcat tacgcttctt cttcctcttc cgtcaggtct aaccttacgt cgtcttttcc
     1681 gcggtcgaag aacattttac gcaacgggtt ttcgcccgct tcatcgtaat cgcggcggtt
     1741 gttaaaaata tctatagcca tataaatcgc ttgccggaaa gattcttccg atgctttgtt
     1801 ttgccctgca atgtcgtacg ccgttccgtg atcgggagat gtccgcacga taggcaggcc
     1861 ggcagtaaaa tttacccccg aatccatggc gatggtctta aacggaacca gcccctgatc
     1921 gtgatacatg gctaacacgg catcaaattc cctgtacctt ccggttccga agaagccgtc
     1981 tgcggcgaaa ggcccggcac acagtattcc tgcatcctgc gcctctttta cggccggagc
     2041 aatgatccgg agttcttcct gtcccagcag tccgttttcc cctgcatgag ggttcaatcc
     2101 gagtacggca atgcgtggtg tttcgatacg gaaatcgcgt ttcagggaga agttgagtgt
     2161 ggacagtttt tcggtgatga gttctttcga tattttttcc gatacttccg ccagaggaat
     2221 atgggtggtg accaacgcaa ttcgtaatga attactggcc aggatcatca acgccttatt
     2281 gttttcggca aaacggtcct gcaaaaattc ggtatggccg gggaaatgga atttatcgtt
     2341 ttgtatggta tctttattga tggggagggt gaccagcacg tcgatttttt ttgcggcaag
     2401 gtccctggcc gcattgtcga gagcgatgaa tgccgactct cccgcttcgg ccgttgccgt
     2461 tcccaactca atcctcacct cttccttaac gcagttgagc aggttaatct tccccgggct
     2521 gcactcatca acatgattga taatgtggaa gctgacgggg ctgtgatcga gtactttacg
     2581 gtgataagag gccgatttgg aagagccgta gatgaccggg atgaaaagtt cctgcatgcg
     2641 ctcatcggca aaggtcttca gcaatatttc gtatcctacc ccgttaatat ctccgtgagt
     2701 gatacccact tttatttgtt ctgacattgt tcaaatttat ttagctgtta ttgcaatgag
     2761 tcgtctactt cttcgctttg ggaaaactta tggtataaag tgctccttcc atgctgcgga
     2821 tcaggttgcg cttgtcggct tccggagcga aaacaaagac ctctaccacc actaccatgt
     2881 tggtctcttc attcacaaag gcatgcgaaa cgaatggacc tcccatcata tcactggtca
     2941 tttcccacaa tccacgcagc tcggcacgga agatgccgtt gtactccatc ttccggtagt
     3001 cgggattata agcctgtgtg gcggtggtca tgtgcgagtc gaaagagccg gtaatgtatt
     3061 gtcccagcac ccggttacgg ataccgatga gggaatcttt ttcgaaaaca gactcggacg
     3121 tatagggaaa ctgatagatc acgatgtctt ttctggccct gccagcactg ttggttgccc
     3181 agtaaaaatt aggatgttcg gtaatgttgg ataatccttt tggatagtaa atgttaaagc
     3241 caaacacctg tttaatatgc gattgcggcg taccggaatc cttgataagc cattgggccg
     3301 tacgctccat ttccttgttt aggatatagt tgacaatacc ttccttgttc tcttttagaa
     3361 aattgataaa cgctgcggta tccggagccc tgacggtcat gattacctgt cccgatgcgt
     3421 acttgtcgag ttcggaagaa agtttggcaa cgctgtaaat ggatgatatt tccggcataa
     3481 tgatgttccg cgccactttg aatgtgctgg taaagttctc cggtgtgagt tgtatgaccc
     3541 tgaaattcgg ctcaaactgg ggcaacccct tggcagggga gttcagcacg tcgaagagtg
     3601 cacgccctgt ttcgccttcc cattcgtctt catccataat gaccaacacc tcgctcgaca
     3661 ttgcagaggc cgacagaaat ccgctgccgt cgcacgatga gagcaggagt attgccaaag
     3721 ccggcaacag gagtaaatga attcgtctca tactgttttc tttagaattt atttttaaca
     3781 aaattacaat tatttgattg taaatgaaat aaatcgtgtt gatttaggat tatttattat
     3841 ttacatgtag ttgttaagac gatgaatatc ccatgacggg cacctatcag tcttgtatcc
     3901 cggttcttgg ttcttttgtc gttgacagca tgccgcaggc ggcaaaaata tcttcgccac
     3961 gcgatgcgcg aatcgtgcag gtgaaccctt tggctgtgag gaaatcgcgg aacgcgatca
     4021 tcgtctcttc cgtggacggt gagaggtcgc tgccgggaat tttatgaaat cggatcaggt
     4081 tgactcgaca atccaatccc gataaaaact tactcagctc gcgggcatac accggggcgt
     4141 cgttcacccc gtcaaacatg atgtattcga acgataaacg gcgctgatgg ctccagtcgt
     4201 actcccggag cagctcgatt atatcggctg ccttaaacgt cttctctatc ggcatcagct
     4261 tttcgcgctg tacagagagc gggctgtgaa ggctcaccgc caggtggcac ctgctttctt
     4321 cgagaaacct tttcaggctg gggatcaccc cgatggtgga tagtgtgatg cgtttggggc
     4381 tccacgccag tgcgtaatcc gcagtgagca cctcaatggc tttcatgacg ttgtcgaaat
     4441 tgttcaacgg ttcacccatg cccataaaga ccaggttggt gagtgcttca gcattgggga
     4501 ctgaataaac ctgattcaat atctcattca ccgaaaggct ccccagaaac cccattttcc
     4561 ccgtcatgca gaagctgcaa tccatcttgc agcccacctg cgatgaaaca cagattgtca
     4621 ggcgttcctc ctccggtatg ttcaccgatt cgatgaactt gccgtttttt acggggaaca
     4681 gcatcttctg cgtcccgtca acggatgttt ttacgtcaac aggctcaacg cggcc
//
LOCUS       DAOSDR010000252         4718 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrimonas sp. isolate AMR_MDS_1826
            NODE_6406_length_4718_cov_3.967832, whole genome shotgun sequence.
ACCESSION   DAOSDR010000252 DAOSDR010000000
VERSION     DAOSDR010000252.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863355
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrimonas sp. (wastewater metagenome)
  ORGANISM  Petrimonas sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Dysgonomonadaceae; Petrimonas.
REFERENCE   1  (bases 1 to 4718)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4718)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:10:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,720
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 35
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4718
                     /organism="Petrimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6406_length_4718_cov_3.967832"
                     /isolate="AMR_MDS_1826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2023866"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..144
                     /locus_tag="PLI30_09820"
     CDS             <1..144
                     /locus_tag="PLI30_09820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006843456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HOI79888.1"
                     /translation="YLPEVYASKVIEISRSFNVDAQVVGRVEKSNIRELVIESEFGRF
                     VYS"
     gene            236..1321
                     /gene="prfA"
                     /locus_tag="PLI30_09825"
     CDS             236..1321
                     /gene="prfA"
                     /locus_tag="PLI30_09825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HOI79889.1"
                     /translation="MSENSLLDKLNHLVTRFEEIGTLITDPTIIGDMKRFVKLNKEYR
                     ELEEIVEARNEYKQALDSIEEAKIILETESDTDLRLLANEELSANTQKLPELEEKIKL
                     LLIPADPEDEKNVIMEIRGGTGGDEASIFAGDLYKMYTRYCESKGWKTEVSSYTEGTA
                     GGFKEIIFTVSGENVYGTLKYESGVHRVQRVPATETQGRVHTSAATVAVLPEAEEFDV
                     EINPADIDFHTARSSGAGGQNVNKVETKVQLTHKPTGIVVVCQQARSQLANRELAMQM
                     LRAKLYDIELTKRQGDIASRRKTMVSTGDRSAKIRTYNYPQGRITDHRINYTVYNLSA
                     FMDGDIQDVINQLIVSENAERMKEAEI"
     gene            1366..2085
                     /locus_tag="PLI30_09830"
     CDS             1366..2085
                     /locus_tag="PLI30_09830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI79890.1"
                     /translation="MKQKILLPTLLLLISVSLSAQTEYKIPRLRISTSGGLGYLVAEG
                     STGDAGSIVNVEKITQFNNDLRWTPSLNADVHYLFKDWGLGVKYIFNTSSATATDLIV
                     DVQDNQHYLVGDALEKMYVNYVGLSVSGQYRFGREERFQFFASSSGGYARLRDESHVL
                     MRNMLITGNAYGVNYEMGIEYFLNSNFSWGLNTAFFWTNFYTVKVTDGISTEVKKLGR
                     EDNINVSNVNLTTGLRYYINK"
     gene            2096..3814
                     /locus_tag="PLI30_09835"
     CDS             2096..3814
                     /locus_tag="PLI30_09835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI79891.1"
                     /translation="MKKKIIYLLTVVVLFISSSCNNDFLEENKKQVDGYHLDTPLFVQ
                     PVSAYTEASVTLPDLKNKGFRILQYPKIIHFETFDGHIDESGKLSFRIKVDAYDNPVQ
                     LEPQNLGSIILNVEEFGLLSINVQSINWGTPKASVRETLVDFDLFRDRKELRIENFAD
                     GILFYKLTKKPSWLEVSKSSWDSDNIKTGDVNYLEPNRYYTLFLIPDADELSPGVHEG
                     EIVFETSDPEHPLLKINVKIRERTYENPETMIPIEGEVVDAEFDKTTNTAVFITRNPA
                     KLVAFNADTGMKKEKLLDKNPYCVGLSAESNTILLGESGQMKFIDLSTFTVKEKVVLP
                     YIVADIVDGENGHYYYSNKEGEIYSFNLSSKEVKKQSIADSFGYGIKADILLKVGNKS
                     QLALTGSFVSPNGFYLIDAAVPDDLKLVHYWHESFGKKLLTSEDCKYLFSFTSSIYKF
                     PDENTGNTIHLLGELSLQSHTGITWMHHSALTSSVWISYSPYDPSQSWFKEVIAEYDD
                     KTFDLKRTIALNEYVATYNGTKDYYHTSARYFFSTKAGNKLFLIKNIDVASPPADTWH
                     IEIIDV"
     gene            3908..>4718
                     /gene="pyrF"
                     /locus_tag="PLI30_09840"
     CDS             3908..>4718
                     /gene="pyrF"
                     /locus_tag="PLI30_09840"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008759883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HOI79892.1"
                     /translation="MTKQQLFAQIQKKKSFLCVGLDTDIKKIPEHLLEKEDPVYAFNK
                     EIIDKTAPYCVAYKPNLAFYESQGVTGWLAFEKTVAYIRQRYPDQFVIADAKRGDIGN
                     TSEMYARTFFETARVDAVTVAPYMGEDSVTPFLNYNGKWVILLLLTSNKGSQDFQFVQ
                     DQNGERLFEKVLRRSQAWASEDQMMYVVGATQGKLFEEVRKLAPDHFFLVPGVGAQGG
                     SLEEVCRYGMNTACGLLVNSSRSIIYADSTETFAEAAGKEARKLQVEMAEML"
BASE COUNT         1457 a          942 c         1085 g         1234 t
ORIGIN      
        1 tacctgccgg aagtttatgc ttcgaaggtg attgagattt ccagatcgtt taatgttgat
       61 gcacaggtag ttggacgtgt ggagaaatca aatatccggg aattggtgat tgagagtgaa
      121 tttggaaggt ttgtttattc ctaaatcctc attcgttgta cctcccaatg gaaaaacata
      181 atatcatcaa acaaaacgat taaattcctt tcaagaaaat cagaggcatc atactatgtc
      241 agaaaactca ttattggata aactcaatca tttggtcacc cgtttcgaag agataggaac
      301 gttgattacc gaccctacaa tcataggcga tatgaagcgt tttgtaaaac tcaacaaaga
      361 ataccgggag ttggaagaaa ttgtggaagc ccgaaacgag tataaacagg cactagactc
      421 catcgaagaa gccaaaataa ttctggaaac cgaatcggac actgatttac gtttgcttgc
      481 caacgaggaa ctctctgcca acacccagaa acttccggaa ttggaagaga aaataaagct
      541 tttgcttatt ccggccgatc ctgaagacga aaaaaatgtg atcatggaaa ttcgtggcgg
      601 tacgggaggc gatgaagcat cgatttttgc aggggatctg tataaaatgt acacccggta
      661 ttgcgaaagc aaagggtgga aaaccgaagt atccagttac acagaaggta cagcgggtgg
      721 tttcaaggag atcatattta cggttagtgg cgaaaatgtt tacggaactt tgaaatacga
      781 gtcgggcgtt caccgggtgc aacgtgttcc ggcgaccgaa actcaaggcc gtgtgcacac
      841 ctcggctgcc actgttgccg tgctgcctga ggcggaagaa tttgatgtag aaatcaatcc
      901 tgccgatata gattttcata cagcacgttc cagtggcgcc ggagggcaga atgtaaacaa
      961 ggtggaaaca aaggttcagc ttacccataa acccaccggt attgtggtgg tttgccagca
     1021 ggcccgttcg caattggcta accgtgaact ggccatgcag atgttgcgtg ccaagctata
     1081 cgatattgaa cttaccaaac gccagggcga catagcttcg cggcgaaaga ccatggtgtc
     1141 caccggtgac cgttcggcta aaatccgcac gtacaactat ccgcagggac gcattaccga
     1201 tcaccgcatc aattacaccg tttataacct ttccgcattt atggacggtg acatccagga
     1261 tgtcatcaat cagcttatcg taagcgaaaa tgctgaacgg atgaaggaag cggaaatcta
     1321 gttgaatcta gacaaaaaca caagttatat ttaattatat gacctatgaa acaaaaaatt
     1381 ttgttaccga cacttctgtt gttaatcagc gtatctttat ccgcacaaac ggaatacaag
     1441 ataccgcgtt tacgcattag tacatcggga ggattgggtt atttggttgc ggaaggaagt
     1501 accggcgatg ccggttctat cgtcaatgtt gaaaaaatca cgcaattcaa caatgatttg
     1561 aggtggactc cctccttgaa cgcagatgtg cattatctat tcaaagattg gggattggga
     1621 gtgaagtata ttttcaatac ttcctctgcc acggcgacgg atctcattgt ggatgtccag
     1681 gataaccagc attatctggt tggcgatgca ttggaaaaga tgtatgtaaa ctacgtggga
     1741 ctttctgtat caggacaata caggtttgga agagaagaga gatttcagtt ttttgcttct
     1801 tcttcaggcg gttatgcccg attgcgggac gaaagccacg tattaatgag gaacatgttg
     1861 attacgggaa atgcttacgg tgtaaattac gagatgggaa ttgaatattt tttaaattcg
     1921 aacttttcat ggggattgaa taccgcattt ttctggacaa atttttatac ggtgaaagtg
     1981 acggacggta tcagtaccga agtgaaaaaa ttgggaagag aagacaacat caatgtttcc
     2041 aacgtaaatt taacaaccgg tctgcggtat tacatcaaca aataaatgaa gcgtcatgaa
     2101 aaagaagatc atttatctgc ttactgttgt tgtcctgttc atttcctctt cgtgcaacaa
     2161 cgattttctg gaagaaaaca aaaaacaggt tgatggatat catctcgata cacctctttt
     2221 tgtgcagcct gtctcagcat acaccgaagc ctcagttacc cttcccgatt taaaaaacaa
     2281 aggtttcagg atactccagt atccgaaaat tattcatttt gaaacttttg acggacatat
     2341 agatgagtcg ggaaaattgt cattccgaat aaaagtggat gcatacgata atcccgttca
     2401 gctggagcca caaaacctgg gaagcatcat tttaaatgta gaagaatttg gactcctgtc
     2461 tataaacgtt caaagcataa actggggaac gccaaaagca tccgttcgcg aaacgctggt
     2521 tgactttgat ttgttcaggg acagaaaaga acttagaatt gagaattttg ccgacggaat
     2581 acttttttat aaattgacga aaaagccgtc gtggctggag gtgagcaagt catcctggga
     2641 cagtgataat ataaagacag gagatgtaaa ctatctggaa ccaaacagat actataccct
     2701 ttttctaatt cctgatgccg atgaattaag cccgggagtg catgaaggtg aaattgtctt
     2761 cgaaacatcc gacccggaac accctcttct caagattaac gtaaaaatta gagaaaggac
     2821 atacgaaaat ccggaaacga tgatccccat agaaggtgaa gtggtggatg cagaatttga
     2881 taaaaccaca aacacagcgg tctttattac ccgcaatccg gcaaaactgg ttgcgttcaa
     2941 tgccgacacc ggtatgaaaa aagaaaaact gttggacaaa aatccctatt gcgttggctt
     3001 gtctgccgaa agtaacacaa ttctgctagg agaaagtggg cagatgaagt tcatcgattt
     3061 atccacgttc accgtaaaag aaaaggttgt tctaccgtat attgttgcgg atattgtcga
     3121 tggtgaaaat ggtcattact attattcgaa caaggaaggg gagatctact cctttaacct
     3181 ttcgtccaaa gaggtaaaaa aacagtccat tgccgattca ttcgggtatg gaataaaagc
     3241 ggatatccta ttgaaagtag gaaataaatc gcagttggca ttaaccggaa gttttgtttc
     3301 cccaaatggc ttttatctga tcgacgcagc cgtgccggat gatttgaaac tcgtccatta
     3361 ttggcacgaa agctttggaa agaaattact gacctccgaa gattgcaaat acctgttttc
     3421 ttttaccagt tccatctaca aatttccgga tgaaaatacc ggcaatacga ttcacctgtt
     3481 gggtgaatta agcttgcagt cacatactgg aattacctgg atgcatcatt cggccttaac
     3541 ctcttctgtt tggatctcct acagtcctta tgatccttcc caaagttggt ttaaagaggt
     3601 tattgcggaa tacgacgaca aaacctttga cctaaaacgt actatcgcac taaacgaata
     3661 tgtggcgaca tacaatggaa caaaggatta ttaccatacg tctgcccgtt attttttctc
     3721 caccaaagcg gggaataaat tatttctgat aaaaaatatc gatgttgcta gtcctccggc
     3781 cgacacttgg cacatcgaaa ttattgatgt ttaaaaatag cactatcgcg ttgtgccata
     3841 aaagcagcag ccaaacttgt ctgaattatg ccacggcgag caatattctg ataaaattga
     3901 acaagatatg accaaacaac agctttttgc acaaattcaa aaaaagaaat cttttctttg
     3961 tgtgggattg gataccgata tcaagaaaat tccggaacat ctgctggaaa aagaagatcc
     4021 ggtttatgcc tttaacaaag aaattatcga taaaacggca ccttattgcg tggcatacaa
     4081 gcctaacctg gctttttacg aaagtcaggg cgtgaccggt tggctagcgt tcgaaaaaac
     4141 ggtagcctac atcaggcagc ggtatcccga ccaatttgtg atagccgatg ccaaacgggg
     4201 cgatatcggc aataccagtg aaatgtatgc tcgtacattt tttgaaactg ccagggtgga
     4261 tgccgtaact gtagcaccgt acatgggcga agacagcgta actccttttc tgaattacaa
     4321 cgggaaatgg gttattctgc tccttcttac aagcaataaa gggtcacaag attttcagtt
     4381 tgttcaggat caaaacggtg agcgtttgtt tgagaaagta ctccgcaggt cgcaggcatg
     4441 ggcgtcggaa gaccaaatga tgtacgttgt gggcgctaca cagggaaaat tattcgaaga
     4501 ggtaagaaaa ctcgctcccg atcatttctt ccttgttcca ggcgtaggag ctcagggtgg
     4561 gtcgctggaa gaggtttgcc gatacggaat gaacacagca tgtggcttat tggtcaactc
     4621 ttcgcgcagt attatctatg ccgacagtac cgagacattc gcggaagctg ccggcaagga
     4681 agcccgaaaa ctgcaggtgg agatggcgga aatgctgg
//
LOCUS       DAOSDR010000253         4708 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrimonas sp. isolate AMR_MDS_1826
            NODE_6419_length_4708_cov_4.910380, whole genome shotgun sequence.
ACCESSION   DAOSDR010000253 DAOSDR010000000
VERSION     DAOSDR010000253.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863355
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrimonas sp. (wastewater metagenome)
  ORGANISM  Petrimonas sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Dysgonomonadaceae; Petrimonas.
REFERENCE   1  (bases 1 to 4708)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4708)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:10:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,720
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 35
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4708
                     /organism="Petrimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6419_length_4708_cov_4.910380"
                     /isolate="AMR_MDS_1826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2023866"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..943)
                     /locus_tag="PLI30_09845"
     CDS             complement(<1..943)
                     /locus_tag="PLI30_09845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010991856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOI79893.1"
                     /translation="MTHKYDFLIIGSGIAGMSYALKVAKHGKVAIIAKTTLEDANTYY
                     AQGGIASVTNPWDNFEKHITDTLDAGAGLCDVSVVERVVQEAPAQINELISWGVNFDK
                     DEKGNFDLHREGGHSEFRILHYKDSTGAEIQTSLIETIRNHPNIDVYDNHFTIEILTQ
                     HHLGQVVTRHTPGIECYGAYILDLQTREVLTFLSRIAMMATGGIGCIYQTTTNPLVAT
                     GDGIAMVARAKGEIKGMEFVQFHPTALYHPGVRPSFLITEAMRGYGGVLKTKDGKEFM
                     QKYDERLSLAPRDIVARAIDTEMKNRGDDHVFLDVTHK"
     gene            1188..2453
                     /locus_tag="PLI30_09850"
     CDS             1188..2453
                     /locus_tag="PLI30_09850"
                     /inference="COORDINATES: protein motif:HMM:NF025006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HOI79894.1"
                     /translation="MKNYRIINLLLLLLFSFQLFGDDVLQAQSYTLRGRVLASDTRDP
                     LSNASVTVGSLNISSVTNQDGYFTLRVPASAKNSRLIIRYLGYENLEVPVVTLIDKPN
                     NHIMLIPSFIELGPLEVVSGDGSELIREALSRIPQNYSTDPNMMVAFYRESIKKNANY
                     ISLVETVLDVYKSSYRSYESDQAKIYIGRKATDISPRDTVLLKFQGGISTALMLDVAK
                     NPEIVFGEKGDEYIFTIERMISINNKPHYEINFLPKNGIKDILFRGKVYLDVESLAIV
                     RIEFNMNVEGRKDASNIFIRRKPSKMKVDVEEARYIADFIENNGKWFFNYSSTEVRFR
                     VRWTNRFFGLFATNYTIGSEMAITDRYQESVNKFPRDERIRSTDVIAEKVEHFQDPEF
                     WGEYNVIEPDIEINNAIKRLSGRLMRRGE"
     gene            2541..2810
                     /gene="rpsO"
                     /locus_tag="PLI30_09855"
     CDS             2541..2810
                     /gene="rpsO"
                     /locus_tag="PLI30_09855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007763600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HOI79895.1"
                     /translation="MYLDSEKKKEIFAKHGKSNTDTGSPEAQIALFSYRISHLTEHLK
                     SNKKDYNTERSLKILVGKRRRLLDYLVEKDINRYRAIVKELGLRK"
     gene            complement(3089..3958)
                     /locus_tag="PLI30_09860"
     CDS             complement(3089..3958)
                     /locus_tag="PLI30_09860"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOI79896.1"
                     /translation="MIHYADNTTRQQVYDMWKTVFGDSDEYMEIYFREKYRNENTLIY
                     FESGKAVSSLQMLPFDFSFHGSEIPVAYFSGLCTLPEARKKGFMGALIKKSFGEMDEK
                     GIPLAILVPQDKTVMKFYRQFGFTQTFDAGAPLPDLQKIMVESENLHNAYEIFDSFFR
                     QRDMTVQKTPDDFRAIVEEAALFDFPVKKGLMAMSRITDAEKLLIIFAKKYPQIKVSV
                     KVSDPIIGKNNAVFVIKNGSVSKSSKKETTHFYVEIDALTQLLLGYRTSEFSNDYRLV
                     FPEKQPLIGFMME"
     gene            complement(3964..>4708)
                     /locus_tag="PLI30_09865"
     CDS             complement(3964..>4708)
                     /locus_tag="PLI30_09865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019538458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphatidylglycerol lysyltransferase
                     domain-containing protein"
                     /protein_id="HOI79897.1"
                     /translation="FIRFRDNNNRNSYLKPVGGDNIKRAIETIIDDHQQFDTTFQIRG
                     LTQEMINEIETAMPGAFDYKFNRSVSDYIYTTEKMINLTGKKLQSKRNHINRFKRENE
                     WKYKSLTGNPALVKECKEMLDQWMQINLEEKDPSLVYDDFATTLMLENFEYLELKGGL
                     ICVNNEIAAFTIGEPLTKDTFIVHVEKAFTTIHGAYNIINQQFIENEAADFTYVNREE
                     DMGIENLRNAKLSYQPDILLEKYNARLKN"
BASE COUNT         1290 a          961 c         1031 g         1426 t
ORIGIN      
        1 ccttgtgcgt tacatccaga aaaacatgat cgtcaccccg gtttttcatc tccgtatcga
       61 tggcacgggc aacgatatcg cgcggcgcaa gagaaagccg ttcgtcgtac ttctgcataa
      121 actctttacc gtcttttgtc tttaataccc cgccgtatcc ccgcatggct tcggtaatga
      181 gaaacgacgg acgcacgccg ggatgataca acgccgtggg atgaaactgt acgaactcca
      241 tcccctttat ttcgcctttg gcgcgtgcta ccatggctat gccatcgccg gttgcgacaa
      301 gtggattggt tgttgtttga tagatacaac cgataccgcc ggtcgccatc atggctatgc
      361 gcgaaagaaa cgtcagcact tcccgggtct gtaaatccag aatgtaggca ccgtaacatt
      421 ctattcccgg ggtatgccgg gtgacaacct ggcccaggtg gtgctgggta aggatttcga
      481 tggtgaaatg attgtcatag acatcaatat tggggtgatt ccggatggtt tctatcagac
      541 ttgtttgtat ttctgcacct gtgctgtcct tgtagtgtag gatccgaaat tcggaatgtc
      601 cgccctcccg gtgcaggtca aaatttcctt tttcgtcttt gtcaaaattt acaccccagc
      661 tgatcagttc attgatttgt gccggggcct cttgtacaac gcgttccact acgctgacat
      721 cacacaaccc tgcccccgca tcgagcgtgt ctgtaatgtg tttttcaaaa ttgtcccagg
      781 gatttgttac cgaagcaatg ccgccctgtg catagtaagt gttggcatct tctagcgttg
      841 ttttagctat tatagccact ttgccgtgtt tggccacttt cagggcgtaa ctcatccccg
      901 caatgcccga tccgataatt agaaaatcgt atttatgcgt catgctcttt ttcagatttt
      961 tttcaccaca aatttaggta ttaaagttta gaatttgttt tatatcatca tttaataatt
     1021 gaacttccgt tacggcatca gttaaatctt gcgaatgaca atgtattgtg accttataaa
     1081 ataatcatta aaactctgtg gtttagtttg attgtttaag taaaaaacgt ttactttgtg
     1141 tagcgtatgt aacccgatgg ataaacaatt taaaactttc caaaccaatg aaaaattata
     1201 ggattattaa cttattgctg ttgctgctct ttagttttca gctctttggg gatgacgtgt
     1261 tgcaggcaca atcgtatacg ctgcggggta gggtattggc aagtgacacc cgcgatccgc
     1321 tttccaatgc gagtgtaact gtaggaagcc tgaatatatc gagtgtcacc aaccaggatg
     1381 gatactttac gctcagggtt cctgcatcgg caaaaaactc cagactgatt atacggtatc
     1441 tggggtatga gaacctggag gttccggtgg ttacgctcat cgacaagccc aacaaccaca
     1501 ttatgttaat accttctttc attgaactgg ggccgcttga agtagtttcc ggtgatggaa
     1561 gtgagttgat aagggaagcg ttgagccgta taccgcaaaa ctattctacc gatcccaata
     1621 tgatggtggc gttttaccgg gaatcgataa agaaaaatgc aaactatatt tctttggtcg
     1681 aaaccgtact ggatgtgtat aaatcttcgt atcgttcgta cgagagtgac caggcaaaaa
     1741 tctacatagg ccggaaagca accgatattt cgccgcgcga tactgttttg ttgaagtttc
     1801 agggcggaat cagtacagca cttatgttgg atgtggcgaa gaatcccgag atcgtttttg
     1861 gtgaaaaagg ggatgaatat attttcacca ttgaaagaat gataagcatc aacaacaagc
     1921 cgcactatga gattaatttc ctgccgaaga acggtataaa agatattctg ttccggggaa
     1981 aggtttacct ggatgttgaa tccctggcta ttgtcagaat agaatttaac atgaatgtgg
     2041 agggccggaa agatgcttcc aatattttta tccggcgtaa accttcaaag atgaaagtgg
     2101 atgtggaaga agcccgctat attgccgatt ttatcgaaaa taacggtaaa tggtttttca
     2161 attacagcag cactgaagtc cggttccggg taaggtggac taaccggttc ttcgggcttt
     2221 ttgcaacaaa ctacacgatc ggttccgaga tggccattac ggacaggtat caggaaagtg
     2281 tcaataaatt tccgagggat gaacgaatcc gttctaccga tgtgattgcg gaaaaggtgg
     2341 aacacttcca ggatcctgag ttctggggcg aatacaacgt gattgaaccc gacatagaga
     2401 taaataatgc cataaaaaga ttgagtggga gattgatgcg ccgtggagaa taaatagttg
     2461 taaaatattt gtaatctgaa aatttagctg tatctttgcc tcccaaaaac tttttaaata
     2521 aatttttaca aacaagtttt atgtatttag attctgaaaa aaagaaagaa atctttgcca
     2581 agcacggaaa gtctaacact gatactggct cgcctgaagc gcagatagca ttgttttcat
     2641 accgtatttc gcatttgact gagcatttga agtcaaacaa aaaagattat aacacggaac
     2701 gttcattgaa aattttagtg ggtaaacgtc gtcgtttact ggattacctg gtagaaaaag
     2761 atatcaatag gtacagggct atcgttaaag aattaggcct gagaaagtaa aattttccgg
     2821 aaaaacgaaa aaaagggagt cgacgctatt cagcattact ctcttttttg ttttacatcc
     2881 gtgcggtcag aaatcataca gaattccagc agaaaaaatg gatggcaaat taaaaaatat
     2941 gttataaaac atgtaaatat attttgcagg tatgaaaaaa gaggcttatc tttgtatcga
     3001 taacctaaaa caatcttttt taccttattg acaataacag gataaaccga agacaaaaga
     3061 ggtcatctcg atcgaggtga cctttttttt attccatcat aaagccgatc aatggctgtt
     3121 tttcaggaaa aacaaggcgg taatcatttg aaaattctga agttctataa ccaagaagta
     3181 gctgggtcag cgcatctatc tctacataga aatgcgttgt ttcttttttg ctgcttttgg
     3241 agacactccc gttttttatc acaaaaacag cattattttt cccgataatg gggtctgaaa
     3301 cttttacaga aacttttatt tggggatatt tttttgcgaa aattatcaac agcttttctg
     3361 catctgtgat gcgcgacatg gccatcaggc cttttttgac tggaaaatca aacaatgcgg
     3421 cttcttccac aattgcacga aaatcatccg gcgttttttg cactgtcatg tcgcgttgac
     3481 gaaaaaaaga atcgaatatc tcgtaagcat tatggaggtt ttccgattca accataattt
     3541 tttgtaagtc gggaagaggg gcacctgcat caaatgtttg ggtaaatcca aattgcctgt
     3601 agaacttcat tactgttttg tcctgcggaa ccagaatggc aagcgggatt cctttttcat
     3661 ccatttcacc gaaagatttt ttgataagag cccccataaa tccttttttt ctggcttctg
     3721 gtagcgtgca tagcccggaa aaataagcta ctggaatttc tgaaccgtga aatgaaaaat
     3781 caaacgggag catctgcagg gaagagactg ccttcccgct ctcgaaatag atcagtgtat
     3841 tttcgttcct gtatttctcc cggaaataaa tctccatgta ttcgtcggaa tcgccgaaca
     3901 cggttttcca catgtcgtaa acctgctgtc tggtcgtatt gtcggcgtag tgtatcatgg
     3961 tgattaattc ttaaggcgtg cgttgtattt ctcgagtaga atatcgggct gataagacag
     4021 cttggcatta cgcaggtttt caatacccat gtcttcttcc cggttaacat aagtaaaatc
     4081 cgctgcttca ttctcgataa attgctggtt gatgatgttg taggcgccgt gtatagtggt
     4141 aaatgccttt tctacgtgaa caataaaggt gtcttttgtg aggggctcac cgattgtgaa
     4201 tgcggcgatt tcgttgttca cacatattaa cccgcccttc agctccaggt attcaaaatt
     4261 ctcaagcatc agcgtagtag caaaatcgtc gtaaaccagc gaagggtcct tctcttccag
     4321 gtttatctgc atccactggt cgagcatttc tttacattcc tttaccagtg ccggattccc
     4381 ggtcaacgat ttgtatttcc actcgttttc gcgcttaaac ctgttgatat gattcctttt
     4441 gctttgtaac tttttcccgg taaggttgat cattttttct gttgtgtaga tatagtccga
     4501 tacgctgcgg ttgaacttgt aatcgaaagc accgggcatt gccgtttcaa tttcattgat
     4561 catctcctga gtcaaccccc tgatctgaaa agtcgtatca aactgctggt ggtcgtctat
     4621 gatcgtttca atggctctct ttatattgtc gcctcccacc ggtttcaggt aggagttccg
     4681 gttgttgttg tccctgaacc ggataaaa
//
LOCUS       DAOSDR010000254         4706 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrimonas sp. isolate AMR_MDS_1826
            NODE_6421_length_4706_cov_3.810793, whole genome shotgun sequence.
ACCESSION   DAOSDR010000254 DAOSDR010000000
VERSION     DAOSDR010000254.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863355
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrimonas sp. (wastewater metagenome)
  ORGANISM  Petrimonas sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Dysgonomonadaceae; Petrimonas.
REFERENCE   1  (bases 1 to 4706)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4706)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:10:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,720
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 35
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4706
                     /organism="Petrimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6421_length_4706_cov_3.810793"
                     /isolate="AMR_MDS_1826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2023866"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(29..1414)
                     /gene="mnmE"
                     /locus_tag="PLI30_09870"
     CDS             complement(29..1414)
                     /gene="mnmE"
                     /locus_tag="PLI30_09870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011109330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HOI79898.1"
                     /translation="MNDIICAVSTPPGMGAIAVIRLSGEGSIAVTDTLFVSPSGKKLA
                     GAKANTVLFGQIVFNNEVTDEVLVTVFRAPHSFTGEESVEISCHGSVYIQQKIVEALI
                     ARGVRLAQPGEFTRRAFGNGKFDLSQAEAVADLIASASQASHRVAMNQMRGGFAGKLA
                     ELRDKLLQFVSLVELELDFPEEDVEFANRQKLYELTEEIEREIDKLARSFQLGNAIKN
                     GIPVAIIGETNAGKSTLLNLLLNEEKAIVSDIHGTTRDVIEDTVNISGVSFRFIDTAG
                     IRHTTDTIETMGIERTFQKIGQASIVLWMIDLTTPVEKIESLAESIVPKLADKHVLLL
                     FNKADLLPDAERVEKEHILPELKAERLFISAKKQQNTDVLQQLLLKAAAIPQIGEHDV
                     IVTNLRHYEALVKALEAVRRVKEGLEIGISSDFISQDIRECMFYLGEITGQISTDEIL
                     GNIFSKFCIGK"
     gene            complement(1427..3097)
                     /locus_tag="PLI30_09875"
     CDS             complement(1427..3097)
                     /locus_tag="PLI30_09875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009123658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside kinase"
                     /protein_id="HOI79899.1"
                     /translation="MTDTVRIHCLNTNEYKDVRVGSSLEELIEVFGVKKPYLIANAKV
                     NNKTESLTYQVYRPRRVEYVDISDSSAMRTYVRSLCFVLSKAVDDTLPDAEVYIEHAV
                     SRGYYFHIESNVEVGEKELMAVKKRMQEIIDADIPFIQVEEEIAEVVKLFRENGMKDK
                     ALLLETSDDLLYARYSKLDNYIDYYYGCLLPSSAYLHLFDVVPYNGGFLLVVPNRQNP
                     VELEPVIPQQKLLKVYREHLEFLKISKLDNVGDLNKAIRTNKISEIIQVSEAYQANEI
                     ADIAKEITERYNDGLRVVLISGPSSSGKTTFRKRLEVQLYVNRLKPVGISLDDYFIDR
                     DLTPFDEFGERDYESLYAIDLELFENQIIALLNEEEIELPSYNFVTGKREFRNRRLVM
                     DKKSVLIVEGIHALNPKLTEHIARDKKYMIYVSALTSISLDNHNWIPPADNRLLRRIV
                     RDNRYRGYSARDTISRWDSVRRGEEKWIFPYQENADVMFNSAMIYELAAIRRHAEPVL
                     QQVPRVVEEYSEAYRLLKFLRYFNYIADRELPPTSLLREFLGGSSFRY"
     gene            3183..>4706
                     /locus_tag="PLI30_09880"
     CDS             3183..>4706
                     /locus_tag="PLI30_09880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009860126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005436 - sodium:phosphate symporter
                     activity [Evidence IEA];
                     GO_process: GO:0044341 - sodium-dependent phosphate
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HOI79900.1"
                     /translation="MDYSFFDFLKLIGSLALFLYGMKIMSEGLQKLAGNKLRTILSAM
                     TKNRVMGVLTGVLITALIQSSSATTVMVVSFVNAGLLSLVQSIGVIMGANIGTTVTAW
                     IISLFGFGKFSISALSIPLMGIALPLIFSSKSKNKSTGEFIFGFAFLFMGLDFLKSAM
                     PDLQNNPEVLSFVQNFSDMGIGSVLIFLLIGSVLTILVQSSSATVALTLIMATKGWID
                     FPAAAAMIMGENIGTTITANLAAIPANLSAKRAAFAHFMFNVMGVIWMLFVFKYFVNM
                     VEGLVTSFGPANPAEMSAFISSLSPEEYTQITTLSKSELSADLVSKQNMYLSYQGATS
                     YGLSLFHTLFNICNVSIMIWFVRVYEKICLNVIRPKAGEEEDEEFVLQYISTGILSTA
                     ELSILQAEKETEVYAKRARKMFGFVKKMTNMDHNDKKFLKLYNRLEKYEDICDRMEVE
                     IGSYLGKVSEGRLSHQSKEHIRAILRAVTEIESIADSCCNIGRHLKRKMEANAVFEEN
                     "
BASE COUNT         1229 a         1193 c         1034 g         1250 t
ORIGIN      
        1 ttattttaac tatttaccaa ttatacagtt actttccgat acagaacttg ctaaagatgt
       61 tccccagaat ttcatcggtt gatatttgcc cggtaatttc cccgaggtag aacatgcact
      121 cacggatatc ctgtgatata aaatcgctgg agataccgat ctccagtccc tctttaaccc
      181 gacggacggc ttccagcgct tttaccaatg cctcgtagtg gcgcaggttg gtcacaatca
      241 catcgtgttc gcctatttgc gggatagccg ccgcctttaa cagcagctgc tgcaaaacgt
      301 cggtattctg ttgtttcttt gccgatataa acaggcgttc ggccttcagt tcaggcagga
      361 tatgttcttt ttcgactcgt tcggcatccg gcaggagatc ggctttgttg aacaacagca
      421 acacatgctt gtcagcaagt ttggggacaa tggattcggc aagcgactct attttctcca
      481 cgggagtagt tagatcaatc atccacaaca caatggaggc ctgcccgatt ttttgaaaag
      541 tgcgttcgat ccccatcgtc tcaatggtat cggtagtgtg acggatacct gcggtatcga
      601 taaaccggaa agagactcca ctgatgttta cggtatcttc aatcacgtca cgcgtggtgc
      661 cgtggatatc ggaaacaatg gctttttctt cgtttaacag caggttcagc aacgtggatt
      721 ttccggcatt ggtctcaccg ataatggcaa cgggaatacc gttcttaatc gcattcccca
      781 actgaaacga acgagccagc ttatcaatct cccgttcaat ctcttccgtc agttcgtaaa
      841 gtttctgccg gttggcaaac tccacatcct cttccggaaa atccagttcg agctcgacca
      901 acgacacaaa ctgcaacaac ttatcccgta actccgccag ttttccggca aacccgccgc
      961 gcatctggtt catggccaca cggtgcgaag cctgcgatgc ggaggcaatc agatcagcta
     1021 ccgcttctgc ctggctcagg tcgaatttac cgtttccgaa cgcacggcgt gtaaactcgc
     1081 ctggctgtgc caagcggaca ccccgggcaa tcagtgcctc cacaatcttc tgctggatgt
     1141 ataccgaacc gtggcacgag atctccacgc tctcctcacc cgtaaacgaa tgcggagcac
     1201 gaaaaacggt aacaagtacc tcatccgtaa cttcgttgtt gaacacgatc tgtccgaaaa
     1261 gtaccgtgtt tgcctttgcc ccagccagtt ttttaccgga tggcgaaaca aaaagggtat
     1321 cagtcacagc aatagatccc tctcccgata agcggataac ggctatcgca cccatcccgg
     1381 gcggagtgga taccgcacag ataatatcat tcatatgcgt tgtgaattaa taccggaaac
     1441 tactccctcc caaaaactcc ctcagcaggg aagtgggcgg caattccctg tcggcaatat
     1501 aattgaaata acgcaaaaac ttcagcaggc gataagcttc ggaatattct tccaccacac
     1561 ggggaacctg ttgcaaaacc ggctcggcat ggcgacggat ggcggccagc tcgtaaatca
     1621 tggccgagtt gaacatcacg tcggcgttct cctgatacgg aaaaatccac ttctcttcgc
     1681 cccggcgaac gctgtcccac cgcgaaatgg tatctcgagc agaatatccg cggtaccggt
     1741 tgtcacggac aattcttcgt aacagccggt tgtccgcagg cgggatccag ttgtggttgt
     1801 ccagcgagat ggaggtgagc gccgaaacat agatcatata ctttttatcg cgagcaatgt
     1861 gttccgtcag cttgggattg agcgcatgaa tcccctcaac gatcagcaca ctttttttat
     1921 ccattacgag acgacggttc cgaaactccc tttttccggt cacaaaattg tacgatggca
     1981 attcaatctc ctcctcatta agcaaggcga tgatctgatt ttcaaaaagc tccaggtcaa
     2041 tagcgtaaag cgattcgtaa tctctttcac cgaactcatc aaacggagtc aggtcgcggt
     2101 cgataaaata atcatcaagt gaaataccca ccggtttcag cctgttcaca taaagctgta
     2161 cttccagccg cttcctgaag gtcgtttttc cagacgatga cggccccgag atcagtacaa
     2221 cccgcaaacc atcgttgtat ctttctgtaa tttctttggc aatatcggca atttcattcg
     2281 cctggtaagc ttcggaaacc tgaataatct ctgaaatttt atttgttcgg atagccttgt
     2341 tcaaatcgcc cacattatcc agcttactta tcttcagaaa ctcgagatgc tccctataaa
     2401 ccttcagcaa tttctgctgc gggatgaccg gctccagctc aaccggattc tgccggttag
     2461 gcaccacaag caaaaagccg ccgttatagg gaacaacatc aaacaaatga agataggccg
     2521 atgaaggcag cagacaaccg taataataat cgatataatt gtccagtttt gagtaacgtg
     2581 catacagcaa atcatcagaa gtctcaagca atagcgcttt gtctttcatt ccgttttcgc
     2641 gaaacagttt taccacttcc gcaatctcct cttccacctg aataaacgga atatcggcat
     2701 cgataatttc ctgcatccgt tttttaaccg ccatcagttc cttttcccct acctcaacgt
     2761 tgctctctat gtggaaatag tatcccctcg aaacggcgtg ttcaatgtac acttccgcat
     2821 ccggcaaagt gtcgtcaacg gctttcgaca gcacgaagca taacgagcgg acgtatgtcc
     2881 gcatggccga agagtcactg atgtcgacgt actccaccct acgtggacgg tagacctggt
     2941 atgtcagcga ttctgtttta ttgttcactt ttgcattcgc aatgaggtat ggttttttca
     3001 ctccgaacac ttcgatcagt tcttccaagg aagaacccac cctaacatcc ttgtattcat
     3061 tggtgttaag gcaatgaata cgaacagtat cagtcatgtt tttttcaatt aattttcaca
     3121 aatatttgca atcaagccta ttttagctat atttgcacgc taatatacgc tatttttgga
     3181 aaatggacta cagtttcttc gactttttaa agcttatagg ctcacttgct ctcttccttt
     3241 acggcatgaa gattatgagc gaaggcctgc aaaaactggc aggaaataag ctcagaacaa
     3301 ttttgtcagc gatgacaaaa aaccgcgtca tgggtgtttt aacgggtgtc ctgatcaccg
     3361 cactcatcca atcttcgtca gccactacgg ttatggtggt gagttttgtt aatgccggat
     3421 tgctgagctt ggtccaatcc atcggtgtca tcatgggagc caacatcggg acaacggtta
     3481 cggcgtggat aatttctctg tttggattcg ggaaattttc catcagcgct ttatccatcc
     3541 cgttgatggg gattgctctc cccttgattt tctcatcgaa aagcaaaaac aaatctaccg
     3601 gtgaattcat ctttggtttt gcgtttcttt ttatgggcct cgatttcctg aaaagcgcaa
     3661 tgcctgacct tcagaacaat cccgaagtac tgtcgttcgt acaaaacttc tccgacatgg
     3721 gtatcggatc ggttcttatt tttctgctga taggatctgt tttgaccatc cttgtgcagt
     3781 cgtcgagcgc aaccgtggcc cttaccctga ttatggcaac aaaaggatgg atcgatttcc
     3841 cggctgcggc agcaatgatt atgggtgaaa acatcggcac cactataacg gcaaacctcg
     3901 cagctattcc ggcaaacctg tcggcaaaaa gagccgcctt cgcccatttc atgttcaatg
     3961 tgatgggcgt catctggatg ttgttcgtat tcaagtattt tgtaaatatg gtggaagggc
     4021 tggtaacgtc cttcgggccg gccaacccgg ctgaaatgtc ggcgtttatc tcatccctct
     4081 ctcccgaaga atatacacag ataacaacac tttcaaaatc ggaactttct gcggacctgg
     4141 tgtccaaaca gaacatgtac ctcagttacc agggagccac ttcatacgga ttatcgctgt
     4201 ttcacacgtt gttcaacatc tgcaacgtat cgataatgat ttggttcgtc agggtatatg
     4261 aaaaaatatg cctcaacgtc attcgtccga aagccggaga agaggaagat gaggaatttg
     4321 ttctccagta catttccacg ggaatcttgt ccacggcgga gctctctatt ctgcaagccg
     4381 aaaaagagac ggaggtttac gccaaacggg cacggaagat gtttggattt gtcaagaaga
     4441 tgaccaacat ggaccacaac gacaagaaat ttttgaagct gtacaaccga ctggagaaat
     4501 atgaagatat ctgcgaccgg atggaagttg aaattggatc ttacttaggc aaggtttccg
     4561 aaggaaggct cagccaccag agtaaagaac atattcgtgc cattcttcgt gccgtgaccg
     4621 aaatagaaag catcgcggat tcgtgctgca atatcggccg ccacctaaaa cggaaaatgg
     4681 aagcaaacgc tgtttttgaa gagaac
//
LOCUS       DAOSDR010000255         4686 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrimonas sp. isolate AMR_MDS_1826
            NODE_6450_length_4686_cov_4.355863, whole genome shotgun sequence.
ACCESSION   DAOSDR010000255 DAOSDR010000000
VERSION     DAOSDR010000255.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863355
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrimonas sp. (wastewater metagenome)
  ORGANISM  Petrimonas sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Dysgonomonadaceae; Petrimonas.
REFERENCE   1  (bases 1 to 4686)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4686)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:10:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,720
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 35
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4686
                     /organism="Petrimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6450_length_4686_cov_4.355863"
                     /isolate="AMR_MDS_1826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2023866"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1420
                     /locus_tag="PLI30_09885"
     CDS             <1..1420
                     /locus_tag="PLI30_09885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI79901.1"
                     /translation="IVRGTYEGESYYYKIDLIDNNKNRYNIERNYHYVVKIQSVTRAG
                     FSSFNDALAGASHNNTALDPVIEKYPMISDGNSKLEVEKTLVILTQPNQTFQVWAKYF
                     PDISSDEVNNTGVTVSLQTGNSAINAASLNFNPTTGIISGVSLGAFPQEPGVALIRVS
                     KGELARTIRVVFRTPFQFDPITINNQNPGSVTYGQSQDAILRFTIPDDFPEDLFPLPV
                     YIHTQGLYPAEAGLEMIVENGQINYIYRATYKGLHRVYFKTNKTVMEENVTLSADYFT
                     GGAVVYTSNLGNITYGSNTPIPSSTAISASVGTIQIVQEGKYLYIPPINPDIHAPVTL
                     TYDRQVASNTNSGIQQSFHERYTVSTTVGNLQNGNALNLGIGSYVVTGRIQYTTRTTN
                     YNSLQPVPSWATLTITESSATGVTAKMLETQRYELIIPATNAETATIRVNYTTGSNTY
                     WSQQDISTYRNTQYLRLRTNGP"
     gene            1612..2112
                     /locus_tag="PLI30_09890"
     CDS             1612..2112
                     /locus_tag="PLI30_09890"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HOI79902.1"
                     /translation="MNKLNNYFSPDSNKIIDTKEIAADYYNGVAQCVLHFSNGKYKVI
                     TNENPNDLLAVNVVSYNKTEQGFVELVLKHYKEATSLDDLAIRCDYKSTKTFTRHFKQ
                     KFNTTPKQWQLSIRKNEVIDYLENTDYPLKKIASLLGFSNVSHLRDFCLKKIGLPPEK
                     IRKLDK"
     assembly_gap    2162..2261
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(2585..>4686)
                     /locus_tag="PLI30_09895"
     CDS             complement(2585..>4686)
                     /locus_tag="PLI30_09895"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOI79903.1"
                     /translation="KQGSYQEALIFLNNAYNTLKETSDKLNWMEVCFTLGNTYKKLSQ
                     YGEAEKYLVEGLEIAKQLNLPFYLKNAYSDLSELYYLQNRPALAQQYHVVSHSFDNLI
                     NRNETQTILFNAFADYEKAESKKQLTGLKTVFDAKTNQQRILIVAFIVIIILLILIFL
                     IHNRYTTMKKQKEKSLVEITKLKSDFYTNITHEFKTPISIIIGLAEKLKKTIGESKSA
                     HNLIDLDIIGRQSENLLFLVNEILTVSKIQTRNSIHWVNGNIVSHLRYLHSCYVDFAE
                     TKKITYLFHSSTDEIIMDYSREQIRVIVNNLLTNAIKHCTAGNKILLMVREDKKQKKC
                     IIEVADNGEGITAKDLPHIFKTFYQGESDLREQMGVGIGLAFTKQVVKSLEGKIQVRS
                     VPFKETVFTVEIPIINHHRASGNPLAEETVPLPRRFEYDPLELEEEKEDSSKPLILIA
                     EDNRDMIFYLTTVLRDEFNIVVAHDGQQAMDFAEEKIPDLIISDLMMPLVDGNKLCSH
                     TKNSVMTSHIPIIVLTAKASDEDRIQLTKAGADAYMTKPFIEEELKAKINQLLKSRKE
                     LREKYGQVVLDSQNASPELTNDSNFEFLQKVTDIIYREMKNIDFFPQGLASEMCVSPS
                     QLNRKIKSISGLNATNYILKVRLNRAKKLLTTSQKPIGEIAMDCGFNDFAYFSRTFKK
                     EFGITPSKYQRMPHLSN"
BASE COUNT         1307 a          950 c          950 g         1379 t
ORIGIN      
        1 aattgtacgg ggtacgtacg aaggggaatc ctactactat aagatcgatt tgattgataa
       61 caataaaaac cgttataaca tcgaaagaaa ctaccattat gtagtaaaaa ttcaatcggt
      121 cacaagggca ggattcagca gttttaacga tgctctggca ggtgcctcgc acaacaacac
      181 agcccttgat ccggtgatcg agaaatatcc catgatctcc gacgggaaca gcaagctgga
      241 ggtggaaaaa acgttggtaa tcctcactca acccaaccag acctttcaag tatgggcaaa
      301 gtattttccc gatatcagca gcgatgaagt gaataatacg ggagtaacgg tctccttaca
      361 aaccggaaac tccgcgatta atgccgcttc ccttaacttc aatccaacaa cagggattat
      421 ttccggggta agtcttggag catttcccca agaaccggga gtagccctga tcagggtatc
      481 gaaaggcgaa ttggcacgta ccatccgcgt ggtgttcagg actcctttcc agtttgatcc
      541 gataacgatc aataaccaaa acccgggatc ggttacgtat ggtcaaagtc aggatgcaat
      601 cttgcgattc accattccgg acgatttccc cgaagacctc tttcctcttc ccgtatatat
      661 tcacacgcaa gggctttatc ctgctgaggc cggtttagag atgatcgtgg aaaacgggca
      721 gatcaactat atctaccggg caacttacaa agggttgcac agggtttatt tcaagaccaa
      781 taaaaccgtg atggaagaga acgtaacact gtcggcagat tatttcaccg gtggagcagt
      841 agtttataca tcaaatctgg gcaacatcac ttacggtagt aatactccga taccctcaag
      901 tacagctatt tctgcttcgg tggggacaat tcaaattgtc caagaaggga aatatctata
      961 tattccacct attaatccgg atatacacgc tccggtcaca ttgacgtatg atcgacaggt
     1021 ggcctctaac acaaatagtg gaatacaaca gtcgttccac gagaggtata ctgtttcaac
     1081 caccgtagga aacttgcaaa acggaaatgc tctcaatctg ggtattggtt cctatgttgt
     1141 aaccggcaga attcaataca caacacgaac gaccaattat aatagtttgc aaccagttcc
     1201 atcgtgggct acacttacaa ttacagaatc atccgctact ggcgtaactg caaagatgct
     1261 ggaaacgcaa cgctatgaac ttatcatacc tgctacaaat gccgaaacag ccaccatcag
     1321 agtgaattat acaactggaa gcaatacata ttggtctcaa caggatataa gtacttatag
     1381 aaatacgcaa tatttacgct tgaggactaa cggaccctaa tgtggagaca ttgcatccgc
     1441 aattaacaaa aaaacaagga atcactattg ggctcatatc taaacaaata ttgttcgatc
     1501 aagagtcttt aataggaaaa tctactgctc aaatcgtttg tttggaatat tcggggaaaa
     1561 acaatttttc tccgaatatc cgacgatcag aggtacaaat tatactaacg tatgaataaa
     1621 ttaaataatt acttttcacc agacagtaat aaaataattg atactaaaga gattgctgcc
     1681 gattattata acggtgtcgc gcaatgtgtg cttcatttca gcaacggtaa gtacaaagtt
     1741 atcacaaacg aaaacccgaa tgatctgctt gcagtgaacg tggtaagcta caataagacc
     1801 gaacagggat ttgtggaact ggtattgaaa cactacaagg aggccacgtc acttgacgac
     1861 ctggcaatcc ggtgtgatta caaatctacc aaaacattca cgcgccattt caagcaaaaa
     1921 ttcaacacca ctcccaaaca atggcagttg tcaatccgaa agaatgaagt catcgattac
     1981 ttggagaata ccgattaccc gttaaaaaaa attgcatccc ttttgggttt ttccaatgta
     2041 tcacacctgc gcgatttctg tctcaaaaaa atcggcttgc cgcccgagaa aataagaaaa
     2101 cttgataaat gaaaagagcg aactgtccca cactacagtt cgctctctgt ttaaatttcg
     2161 gnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2221 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn naataagttc tttgttaaat
     2281 ttattattca atttgctgtt cggtttaaac cagaccagtc aataaatcga acgagcggtc
     2341 aagaaactgt atataaagtg tttatatggt ttttttcttt cctgtgctag cttaaaagag
     2401 ttaaagtatg cattgaggtt aaacaatggc tttgcaatat tccttgcctc agatcgcatt
     2461 gatctggtgt aacttttagc gtcttcgaag cgttccattt aagaatagtt acggtgttag
     2521 tatttcaatc tttttcttca gaaaaataga aaaccggtat tcttttaagg attatgaatg
     2581 aaatttaatt tgataggtgg ggcatgcgct gatatttgga aggggtgatt ccaaattcct
     2641 ttttaaatgt tcttgagaaa taggcaaaat cattgaaccc acagtccatc gcaatttctc
     2701 cgataggttt ctgggaagta gtcagcagtt ttttggcacg gttcagccgt accttcagga
     2761 tgtaatttgt ggcattcaat ccagatatgg atttgatttt ccggttcaac tgagacgggc
     2821 tcacacacat ttctgaagcc agcccctgcg ggaagaagtc aatgtttttc atttcccggt
     2881 aaataatatc ggtaaccttt tgcaaaaatt cgaagttgga atcgttcgtc agttcaggtg
     2941 aggcgttttg actgtctaaa acaacctggc cgtatttttc ccttaattct ttcctgcttt
     3001 ttaagagttg gttgattttc gctttcagct cttcctcaat gaacggttta gtcatatagg
     3061 catcggctcc cgctttagtt aattgaattc tgtcttcatc agaagctttg gctgtaagca
     3121 cgattatagg aatgtggctg gtcataacag aatttttggt gtgggagcag agtttattgc
     3181 catctactag gggcatcatt aaatcggaaa tgatgaggtc gggaattttt tcttccgcga
     3241 aatccatcgc ttgctggccg tcgtgcgcca ctacgatatt gaattcatcg cgaaggacgg
     3301 tagtcaggta aaagatcatg tcgcgattat cttctgcaat cagaatcaac ggcttgctgg
     3361 agtcctcctt ctcttcttct aactccaagg ggtcgtattc gaaacgacgt ggcagaggta
     3421 ccgtttcttc tgctaacggg ttccccgatg cacggtgatg gttgataata ggaatctcta
     3481 ccgtgaaaac ggtttcttta aagggtacgc tgcgtacctg tattttccct tccaggcttt
     3541 tcacgacctg ttttgtgaag gcaagcccga ttcctacccc catttgctcc cgtaggtcgg
     3601 attctccctg atagaaagtt ttaaatatgt gtggtaggtc tttggcggtt attccttcac
     3661 cgttatctgc cacttcaata atgcatttct tttgtttttt gtcttccctt accatcagga
     3721 gtatcttgtt tccagcagtg caatgcttga tggcgttcgt cagcaggttg ttgacaatca
     3781 ccctgatttg ttcacgggag taatccatga taatttcatc cgtgctgctg tggaagaggt
     3841 aggttatttt ttttgtttcg gcaaaatcaa cataacagct gtgtagatac cttaaatgac
     3901 tcacgatgtt cccattcacc caatgaatac tgttccgggt ttgaatttta gaaacagtaa
     3961 gtatctcgtt aacaagaaac aagagattct cggattgtct tccgataata tccaggtcta
     4021 tcaggttgtg ggccgatttg ctttctccta tcgttttttt cagtttctcg gccaacccga
     4081 taataatgct tatgggcgtt ttaaattcat gggtaatgtt cgtgtaaaag tcggacttca
     4141 attttgttat ttccaccaac gatttctctt tctgtttttt catcgttgtg tagcggttgt
     4201 gaatcagaaa aatgaggatc aacagtataa ttattacgat aaaggcgaca atgagaattc
     4261 tttgctgatt cgttttggca tcaaacaccg ttttcaggcc tgtgagttgt tttttgctct
     4321 ccgctttttc ataatcggca aaagcattga acagaatggt ttgtgtttcg tttcggttaa
     4381 tcaggttgtc gaaactgtgc gatacaacat ggtattgttg ggcaagtgcc ggcctgtttt
     4441 gtaaataata gagttccgat aaatcggagt aagcattctt caggtaaaag ggcaggttta
     4501 actgtttggc aatttccagt ccttccacaa ggtacttttc agcttcgccg tattgggaaa
     4561 gctttttata tgtatttcct agagtaaaac atacctccat ccagtttaat ttgtcacttg
     4621 tttctttcaa tgtgttgtat gcattgttca aaaaaatgag cgcttcctga taactgccct
     4681 gcttcg
//
LOCUS       DAOSDR010000256         4683 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrimonas sp. isolate AMR_MDS_1826
            NODE_6458_length_4683_cov_4.076707, whole genome shotgun sequence.
ACCESSION   DAOSDR010000256 DAOSDR010000000
VERSION     DAOSDR010000256.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863355
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrimonas sp. (wastewater metagenome)
  ORGANISM  Petrimonas sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Dysgonomonadaceae; Petrimonas.
REFERENCE   1  (bases 1 to 4683)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4683)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:10:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,720
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 35
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4683
                     /organism="Petrimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6458_length_4683_cov_4.076707"
                     /isolate="AMR_MDS_1826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2023866"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..644
                     /locus_tag="PLI30_09900"
     CDS             <1..644
                     /locus_tag="PLI30_09900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005637692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SusC/RagA family protein"
                     /protein_id="HOI79904.1"
                     /translation="PSDGKKIAGRKHIGDYEWKNRPGSQTRNDKDYIDSQDQFFLGYT
                     VPHSTGGLNNNLTFRNISLNVFVDWALGHSINNNSEMRYFMNTFANNYTLIDKVKQTW
                     KAPGDKTKYARFTANDPDDGNANFSRTSNIFNYKGDYLCIREISLQYNVPSSKLGKLG
                     MQNLAITLAGNNLHYFTAVEGVSPEVGASTTYNAGYYNYPPIRKYSIGVKVTF"
     gene            668..2122
                     /locus_tag="PLI30_09905"
     CDS             668..2122
                     /locus_tag="PLI30_09905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007135915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HOI79905.1"
                     /translation="MKSKIIICLMSFFTLLTACHGDLDIVQKSEVSANSMWQDEGDAT
                     SAIYGLYNRFRSTFEQGYMYWGEYRTGLWADGLAGQTARDQIYQNQIPTNHGYANWTD
                     LYTTINQANLILKYTPEIEFVSEETKNKVLANAYFVRAFCYYWIGRIWGDAPVLLEGF
                     ESDKQEGLFPGRDAADLVFKQVGDDINAALSHMPASVTDRKLASQASINMLKADYNLW
                     MFKVRNGGDAALNNAATAVAAVLGNGNYSLVTDYSSIFKNEGGPEVIFAWSYLQDEHT
                     SGATNDWLVPSQYISPEYIENPVKVGSHQQWCFYTADYKALLSEYENDRRTIVSFQTF
                     FDDKKNGTFQWINKYAGSWVSGTRIFDSDVIVYRYADAVLFDAEIKNAKNDRNGAVTA
                     LNKIAKRAYGVDSFYSNTLTASEIDALILKERMKEFAAEGKLWWDYIRFDVVFEKNPF
                     LVGRENEQNILLWPVAQASINRNPNLNQTPGYDK"
     gene            2189..3646
                     /locus_tag="PLI30_09910"
     CDS             2189..3646
                     /locus_tag="PLI30_09910"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HOI79906.1"
                     /translation="MNINSSICNLKFGLFFHLLFLSCSNRDEAKEQIPNVLFIAIDDM
                     LPVLGCYGNSTALTPNIDTLANQGVLFMNAYCQVGLSNPSRASLMTGMRPDEIKVWTL
                     KPHFREEKPNVVTLPQFYKEKGYATRAVGKIYHDGAYHQDPVSWSNPSVLSVTRDGRG
                     HKYVLPENYMPKRSKATTTENANVQDTAYIDGKVCQAAIELLNQLTDSTFFLAVGFRR
                     PHLPFSAPKKYWDLYEREMFKKELKNPRRPARAPDFAFHNSNELRGYEDIGELGVIRE
                     EKQLELLHGYFASVSYIDAQIGKLIAELKRLGRYDNTIIVLYSDNGFHLGDFGLWGKT
                     TNYEASVNIPLIFCGKGIEQGAINRSMVELIDIYPTLIDLTHGEMKSSHDGISLLPIL
                     KGKSSKVKSYALSQIVRPYQDAINSRSPDSMGYSLRTEKYRYIEWRNTKNMSVFAREL
                     YRMDDALSEKENIASDIKNEALMNQFSDIVSRIRK"
     gene            3727..>4683
                     /locus_tag="PLI30_09915"
     CDS             3727..>4683
                     /locus_tag="PLI30_09915"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="HOI79907.1"
                     /translation="MRKNLLLISSILLVLPFLSLKEKEDIDQTVLWEQGMGEYNNYRI
                     PALVVTNEGTLLAFCEGRESGDTGDINLLMKRSEDNGMTWSNEQVVWDDAKNTCGNPC
                     PVVDEETGRIWLFMSWNNGKDKESDIINKTSLSSRLPYVCYSDDDGKTWSKPASLEET
                     CRDPSWGWYATGPGIGIQVKKGAYKGRLVIPANHSYDDPQGNLAGGPYGYGAHVIYSD
                     DHGKSWQISESIKPGCNESQVTELSDGTLLMNMRSYNNKQARAISYSTDGGQNWSEIE
                     HDFQLVESLCQAAVLNFGEVDGQGVHLFLNSAVPHGRDHLTLK"
BASE COUNT         1486 a          843 c         1064 g         1290 t
ORIGIN      
        1 tcccttcaga cgggaagaaa attgccggaa gaaagcatat cggagactat gaatggaaga
       61 acaggccagg tagtcagacc cgaaacgata aagattacat tgacagtcaa gatcagtttt
      121 ttctggggta cactgttcca cactctacag gaggtttaaa taacaatctt acattcagaa
      181 atatatctct aaatgttttt gttgattggg cattgggaca ctcaatcaat aacaactctg
      241 aaatgcgata tttcatgaac acctttgcca ataattacac attgattgat aaggtaaaac
      301 aaacatggaa agctcccggt gataaaacaa aatatgctcg ttttacagcc aatgatccgg
      361 atgacggaaa tgccaatttt agtcgtacat ccaacatctt caactataaa ggtgattacc
      421 tttgtattcg tgaaattagc ctgcaataca atgtaccttc ttcaaaatta ggtaaactgg
      481 gtatgcaaaa tctggccatt accttagctg gtaataatct gcattatttc accgcagtgg
      541 aaggcgtttc acctgaagta ggtgcatcca ccacatataa tgctggttat tacaattatc
      601 ctcctattcg taaatattct atcggagtaa aagttacatt ctaaatcaca ctacaaaaat
      661 gagaattatg aaaagtaaaa tcatcatatg cttaatgtcc ttttttacgt tattgacagc
      721 ttgtcacggt gatctggaca tcgtacaaaa gagtgaggta tctgccaact ctatgtggca
      781 ggatgaagga gatgctactt cggctatata tgggctatat aacagattca gatctacatt
      841 tgagcaaggc tacatgtact ggggagagta tcgaacagga ttgtgggccg acggacttgc
      901 tggtcagacg gcacgagatc agatttatca gaatcagatt cccaccaacc atggttatgc
      961 caattggact gatttatata cgacgatcaa tcaggctaat ctgatcctga aatatacacc
     1021 agaaatagaa tttgtcagtg aagaaacaaa aaataaggta ttggccaatg cttatttcgt
     1081 gcgtgcattc tgttattact ggataggccg aatttgggga gatgctccgg tattgctgga
     1141 aggttttgag tcagataaac aggaaggatt gttccccggt cgcgatgcgg cagatttggt
     1201 gtttaagcaa gttggtgatg atattaatgc tgcgcttagt catatgcccg cctctgtaac
     1261 cgaccgtaaa ttagcttcac aggcctctat aaacatgtta aaagctgatt ataatctttg
     1321 gatgtttaag gtacgtaacg gaggcgatgc ggcactcaac aatgctgcaa cggctgttgc
     1381 tgcagtactg ggaaacggca attacagctt agtaactgat tattcttcca tttttaagaa
     1441 tgaaggggga ccggaggtta tctttgcctg gagttatctt caggatgaac acacaagtgg
     1501 tgccacgaat gactggctgg tgccctccca gtatatttcc ccagaatata ttgaaaatcc
     1561 tgtgaaagtg ggaagtcatc aacaatggtg tttttatacg gccgattaca aagcattgct
     1621 ttcagagtat gaaaatgacc gacgaaccat tgtaagtttc cagacgttct ttgatgataa
     1681 gaaaaatggt actttccaat ggataaacaa gtatgctgga tcctgggtaa gcggtactcg
     1741 tattttcgat tcggacgtga tcgtatatcg ttatgcagat gctgtactgt ttgatgcaga
     1801 aattaaaaat gcaaaaaacg accgtaacgg agctgtgact gctctcaaca aaattgccaa
     1861 acgtgcctat ggagtcgata gtttttattc gaatacgctt acagcttccg aaattgatgc
     1921 attaatactg aaggagcgaa tgaaagaatt tgcagcagaa ggaaagcttt ggtgggatta
     1981 tatccgtttt gatgtagttt ttgaaaaaaa tccgttcctt gtaggtcgtg aaaatgaaca
     2041 aaacatcttg ttgtggcctg tagcacaagc atctatcaat cgaaatccca atctcaacca
     2101 gaccccgggt tatgataaat agaaaataat aagaaggaga ctctctaatt tcatgagtct
     2161 ccctcttatt taaaataacc ataaaagagt gaatatcaat tcgtccatct gtaacctgaa
     2221 atttggattg ttctttcatc tgcttttttt atcatgtagt aatagagatg aagcaaaaga
     2281 acaaataccc aatgtattat ttattgcgat agatgatatg cttccggtat taggttgcta
     2341 tgggaactca acggctctta cacccaatat cgacacgcta gcaaatcagg gagttttatt
     2401 tatgaatgcc tactgtcagg tgggactcag taatccttcc agagcttcat tgatgacggg
     2461 aatgcgaccg gatgaaatta aggtatggac gctaaaacct cattttcgtg aagaaaaacc
     2521 gaatgtagtc acattgcctc agttctataa agaaaaaggc tacgcaacac gtgcagtagg
     2581 aaagatatat catgatgggg catatcatca ggatcctgta tcatggtcga atccttccgt
     2641 actgagtgtt acgcgtgacg gaagaggaca taagtatgta ttacctgaaa attatatgcc
     2701 caaaaggtca aaagcaacaa ctacggagaa tgcaaatgta caggatacgg catatattga
     2761 tgggaaagta tgtcaggcag caattgaatt actaaatcaa ctgacagatt caactttttt
     2821 tcttgcagtg ggtttcaggc gtccccatct tcctttctcg gcgcctaaaa aatattggga
     2881 tctctatgag cgtgaaatgt tcaaaaaaga attaaaaaat ccgagaagac ccgcaagagc
     2941 accagatttt gcatttcata attccaacga gttaagaggg tatgaagata ttggagaatt
     3001 aggagttatc cgggaagaga agcagctgga gctcttgcat ggatactttg caagcgtaag
     3061 ctatatagat gcacaaatag gtaagttgat tgcagaatta aagcgactgg gtcgctatga
     3121 taatacgatc atcgttcttt attccgacaa tggttttcat cttggagatt tcggattatg
     3181 gggaaaaaca acaaattatg aagcttcggt aaatattcca ttaatattct gtggtaaggg
     3241 aattgaacag ggagcaatta atcgatcaat ggtcgagtta atcgatattt atccaaccct
     3301 aattgatttg actcatggag aaatgaaatc atctcacgac ggaatttctc ttttgccgat
     3361 ccttaaagga aagtcctcaa aagtgaaaag ttatgcacta agtcagattg taagacctta
     3421 tcaggacgcc atcaattcga gatcaccaga ttcaatggga tattcattga gaacggaaaa
     3481 atatcgttac atagaatggc gaaatacaaa aaacatgtcg gtgttcgccc gagaattgta
     3541 tcggatggat gatgctttgt cagaaaaaga aaatatagca tctgatataa aaaatgaagc
     3601 attaatgaat caattttccg atattgttag caggattaga aaataggaga atgtatgctg
     3661 tttgtgaaat caaacatcag ttaaagaata acaatacaac aaatggttct ttttaatcta
     3721 tatgatatga ggaaaaattt acttttaata tcgagtatcc tgcttgtctt accgttcctt
     3781 tctttgaagg aaaaggaaga tattgatcaa accgtcttgt gggaacaggg gatgggtgaa
     3841 tataacaatt acaggatacc ggcgctggtt gttaccaacg aaggaacact gctcgctttt
     3901 tgcgagggac gtgagtcagg tgataccgga gatatcaact tattgatgaa acgttcggaa
     3961 gataatggga tgacctggag caatgaacag gttgtatggg acgatgcaaa aaacacctgt
     4021 gggaatcctt gtcctgtggt cgatgaggaa accgggcgaa tctggttgtt tatgagctgg
     4081 aataatggga aagacaagga aagtgacatc atcaataaaa cgtcgttgtc ttctagatta
     4141 ccctatgtct gttattccga tgacgatggc aaaacgtggt cgaagccggc gagtctggaa
     4201 gaaacatgcc gcgacccttc atgggggtgg tatgctaccg gccccggaat agggatacaa
     4261 gtgaagaagg gcgcttacaa aggcaggctg gttattccgg cgaatcatag ttacgatgat
     4321 cctcaaggaa acctggcagg aggcccctat ggctatggag cccatgtgat ctactctgac
     4381 gatcatggaa aatcgtggca gataagcgaa tccatcaagc ctgggtgcaa cgaaagtcag
     4441 gtgactgaat tatccgacgg taccttgctg atgaacatgc ggtcctacaa taataaacag
     4501 gcaagggcaa tcagttatag cacggatggt gggcaaaatt ggtcggagat tgaacacgac
     4561 tttcagctgg tggagtcgtt atgccaggcg gccgtattga attttggtga ggtggacgga
     4621 caaggggtgc atttgtttct caattctgcc gtgcctcacg gaagagatca tctgacgctt
     4681 aaa
//
LOCUS       DAOSDR010000257         4679 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrimonas sp. isolate AMR_MDS_1826
            NODE_6465_length_4679_cov_3.965614, whole genome shotgun sequence.
ACCESSION   DAOSDR010000257 DAOSDR010000000
VERSION     DAOSDR010000257.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863355
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrimonas sp. (wastewater metagenome)
  ORGANISM  Petrimonas sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Dysgonomonadaceae; Petrimonas.
REFERENCE   1  (bases 1 to 4679)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4679)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:10:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,720
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 35
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4679
                     /organism="Petrimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6465_length_4679_cov_3.965614"
                     /isolate="AMR_MDS_1826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2023866"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(227..1498)
                     /locus_tag="PLI30_09920"
     CDS             complement(227..1498)
                     /locus_tag="PLI30_09920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018336901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOI79908.1"
                     /translation="MNEKIAIIEKYNLWGAKTFDFGFKREEYTEKIVDFIGNRLIKVL
                     VGQRRSGKSYILRQVGKQLIDNGVNPENTLFINREFADLDFLRTYKDLDELIKSYKKE
                     LKPKGKVYIFIDEVQIIEQWEKVINSYSQDYSDSYEIFITGSNSKMLSGELATLLSGR
                     YVSFEVFPYSYSEYLRITGQDKGKQSYMAYMDGGGLPELFMLQKQELKRNYISAIKDT
                     VLLRDIIQRNNIRDPKLLEDIFVFLVNNASNLISISSIVKYFKGQGRRISYDAISNYI
                     GYIEDTFLIHRCDRYDIKGKDTLSGNAKFYMNDLAYKNYLYPGFGYGFGYLVENLIYL
                     ELCRAGFDVYVGFLRNKEVDFVAKKADRVIYIQSTYLLSDESTVEREYSALESIGDNF
                     EKIVVSLDDVTLPLRGGIRHLQAWNLKEILR"
     gene            complement(1945..2017)
                     /locus_tag="PLI30_09925"
     tRNA            complement(1945..2017)
                     /locus_tag="PLI30_09925"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1982..1984),aa:Phe,seq:gaa)
     gene            complement(2105..3130)
                     /gene="thiL"
                     /locus_tag="PLI30_09930"
     CDS             complement(2105..3130)
                     /gene="thiL"
                     /locus_tag="PLI30_09930"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009319697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009030 - thiamine-phosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HOI79909.1"
                     /translation="MRTEISTLGEFGLIDHLTKNIILTQPTTKKGVGDDAAVIDNSDK
                     RTLVTTDLLLEGIHFDLTYVPLKHLGYKSAVVNFSDIYAMNGKPQQITVSLGISQRFS
                     VEDLETFYAGVLLACEIYGVDLVGGDTTSSLTGFSISITCVGTADEEKIVYRNGAKDT
                     DLIFVSGDLGAAYMGLQLLEREKAVFGEKADFQPDFSGKEYILERQLKPEARKDIVQF
                     LSGQEIVPTAMIDISDGLSSDLLHICKQSNTGCRIFEERLPIDYQTAVMAETFHMNVT
                     TVALNGGEDYELLFTVPLSLHEKVSALPGVHLVGHITKPEEGCYLVTRDGQEMELKAQ
                     GWNPIGK"
     gene            complement(3142..3954)
                     /locus_tag="PLI30_09935"
     CDS             complement(3142..3954)
                     /locus_tag="PLI30_09935"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005845051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HOI79910.1"
                     /translation="MLEAINQTADYLKNRIGDVPNTAIILGTGLGELAREINDKTEIP
                     YTEIPNFPVSTVEGHSGKLIIGTLGSKRVLAMQGRFHFYEGYNMKQVTFPIRVFKALG
                     VEYLFVSNAAGGMNPSFDVGDIMLIEDHINMFPEHPLHGKNYNELGTRFPDMSEAYNK
                     ELRLMAMEIAKEKNIKLQHGVYVGVSGPTFETPAEYHFFRVIGGDAVGMSTVPEVIVA
                     NHAKMKVLAFSIITDLGVVGKIVEVSHEEVQEAAKIAQPKMAEIMRAIVERM"
     gene            complement(3911..>4679)
                     /locus_tag="PLI30_09940"
     CDS             complement(3911..>4679)
                     /locus_tag="PLI30_09940"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: protein motif:HMM:NF014645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009029 - tetraacyldisaccharide 4'-kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HOI79911.1"
                     /translation="VDKNRRSAIEKIQSIDIEERPQVILLDDGFQHRYVTPSLSILLV
                     DSNRPVYEDKLLPAGNLREPLQGKDRASIVIVTKCSRDMQPIDFRIYENGLDLFPYQD
                     LYFTTFDYGSLIPVFPELQPEILEPDDLRKKHVFLITGIASPQPLVEKLELKTYNLYP
                     KYFPDHHFFKEEDIEEIVREMNTLDVDDDDKMIVTTEKDAVRLRALSFLDEREKKRLY
                     YIPIEVVFLEKNEKESFNKKINKHVRSYQSNSRLSKK"
BASE COUNT         1272 a          983 c          917 g         1507 t
ORIGIN      
        1 ctcatcaaat cttgttctat ctttggtatt ctgtatttga ttactgcttt ttccaagcag
       61 agacactcat tttatttttt caattaactt aattgttttg ttttcttttt aggtaccggc
      121 aaccttgtac gtaaatctgt atcttgcatt tttctaccca attcatcatc tttggctatc
      181 agaaatggat acgtataaga tacaaatatt ccgaatagtc attttttcat cgcaatattt
      241 cttttaggtt ccatgcctgc aaatgcctta ttccacccct tagaggcaat gttacatcat
      301 caagtgatac gacaattttc tcaaaattat caccaataga ttcgagagcg gaatattcac
      361 gctcaacggt agattcatcc gaaagcaaat acgtactttg tatataaatc acacgatcag
      421 ccttcttagc tacaaaatcg acttccttgt tccgtaaaaa accgacataa acatcaaatc
      481 cggcacgaca caactctaag tatattagat tttctacaag atatccaaaa ccataaccaa
      541 atcccggata aaggtaattt ttgtaggcta aatcattcat gtaaaattta gcattacccg
      601 ataacgtgtc ttttcctttt atatcatatc ggtcgcaacg atgaattaaa aatgtatctt
      661 cgatatatcc gatatagtta gatatcgcat catagcttat tcttcgccct tgtcctttaa
      721 aatactttac aatacttgat atggatatca ggttggaagc gttgttcacc aaaaacacaa
      781 aaatatcttc aagcagtttg ggatcacgaa tgttattacg ttgaattata tcccttaaca
      841 aaacagtgtc ttttattgca gagatataat tgcgtttaag ttcttgcttt tgaagcataa
      901 ataattcggg gagtccgccg ccatccatat aagccatata actttgtttg cccttgtctt
      961 gccccgttat tctcaagtat tccgaataac tgtaagggaa aacttcaaaa gatacatatc
     1021 gtcctgatag gagtgtggct aattcacctg aaagcatttt agaattggaa ccggtaatga
     1081 atatttcgta actgtccgaa taatcttgag agtacgaatt gatgactttt tcccattgtt
     1141 ctattatttg gacttcgtca atgaaaatat aaactttacc tttgggttta agttctttct
     1201 tataagattt tataagttcg tctaaatctt tgtatgtacg aaggaagtcc aggtcggcaa
     1261 attcacgatt aatgaacaac gtattttcag gatttacacc attgtcgata agctgtttac
     1321 ccacttgtcg caagatatag cttttgccgg aacgcctttg tccgacaagc actttaatta
     1381 gtctatttcc tataaaatcg actatttttt ctgtatattc ttcgcgttta aaaccaaagt
     1441 caaatgtttt agccccccat aagttatact tttctattat tgcaattttt tcgttcataa
     1501 tcgaatgttt tgcacaaaag tagttctttt tcgtttatgc acgaatatat ttaattattt
     1561 atttaatttt tatttgtcca taatcgaaaa aaatgtatat ttgcaagacc aagaaataga
     1621 tgtttggaga agctctttac gttttagaga aatacaatcg taggtttatc cgtaggggat
     1681 aagtattgtt tgagactgta atatatctga aatacagcgg attaattgat gggtgttccg
     1741 cacccgtcca taccgccaga agctcgtcta aatgaggggc ttttcttttg tagatgccgc
     1801 aagcagccac tttcccacat tttaccccgt ttgtacgaaa tatcattaac tatatggaaa
     1861 acatttcacc gttttcacct gtattttaac agaaattggc ataaaaaagg ggagctactt
     1921 ttttgtaact ccctgatttt caggtggtgc caccaagaat cgaactaggg acacacggat
     1981 tttcagtccg ttgctctacc aactgagcta tggcaccatc gcttttttaa ttgcgactgc
     2041 aaagataaaa ggatttttta caaataaaaa atttttggaa caaaaatatg cggaaattat
     2101 ccggttattt tccaattgga ttccatccct gcgctttcaa ttccatttcc tgtccatcgc
     2161 gggtaaccag gtagcaccct tcttcgggtt tagtgatgtg gcccacaaga tgtacgcccg
     2221 gcagggcaga cactttctcg tgaagagaca agggaacggt aaaaagcaat tcatagtctt
     2281 cgccgccgtt taacgcaacg gttgtgacat tcatatggaa tgtttctgcc attacggcgg
     2341 tttggtaatc gatgggtagc cgttcctcga aaatacggca acccgtgttg ctttgcttac
     2401 agatgtggag gaggtctgac gataatccat ccgagatatc aatcattgcc gtggggacta
     2461 tttcctgtcc ggacaggaac tgaacaatgt ctttccgggc ttccggcttg agctggcgtt
     2521 caaggatata ttctttcccc gaaaaatcag gttgaaaatc cgctttttct ccgaaaaccg
     2581 ccttttcacg ttccaacaat tgcaggccca tgtatgccgc accgaggtcg cccgaaacaa
     2641 atatcaggtc ggtatctttg gctccgtttc ggtatacgat tttttcttca tcagctgtgc
     2701 cgacacaggt tatggaaata gaaaacccgg ttagcgatga tgtcgtgtcg cctcctacga
     2761 gatcaacacc gtaaatttca catgcaagta ataccccggc gtagaaagtt tccagatctt
     2821 caaccgaaaa ccgttgagag atacccaacg aaaccgttat ttgctgtggt tttccgttca
     2881 tcgcataaat gtcggaaaag ttgacaactg ccgatttata tcccaagtgt ttcaacggaa
     2941 cgtaagtcag gtcgaaatga ataccttcca gcaataaatc ggtggtgaca agcgtccgtt
     3001 tgtccgagtt gtctataaca gcagcatcgt ctcccactcc tttttttgtt gtgggttgcg
     3061 tgagtattat atttttggtg aggtggtcta ttaatccaaa ctctcctaag gtggaaattt
     3121 ctgtacgcat aaattaactg cttacattct ttccacaatt gcccgcataa tttctgccat
     3181 tttgggctga gcaatttttg cagcttcctg tacctcttcg tgtgaaactt ccacaatttt
     3241 tccgactaca cctaggtcag taatgatgga aaaagccaat actttcattt tggcgtggtt
     3301 ggcaacgata acttcgggga cggtggacat gcctacggca tcaccgccga taacgcggaa
     3361 gaaatgatat tctgcaggcg tttcaaacgt cgggccggat acgcctacgt acacaccgtg
     3421 ctgtaacttg atgtttttct ctttggcaat ttccatcgcc attaaccgga gttctttgtt
     3481 gtaggcttcg ctcatatcgg gaaatcttgt tcccagttcg ttgtagttct tgccgtgaag
     3541 cggatgctcg ggaaacatgt taatatgatc ttcaatgagc atgatgtcgc ccacgtcgaa
     3601 gctcggattc attccgccgg cagcgtttga tacgaaaaga tattcaacac ccaaagcctt
     3661 gaaaacgcgg atgggaaaag ttacctgttt catgttgtat ccttcgtaaa agtgaaaacg
     3721 cccttgcatg gccagtactc ttttactgcc gagtgttccg atgatgagtt ttccgctatg
     3781 accctccacg gtcgaaaccg ggaagttggg gatttcggtg taaggaattt cagttttatc
     3841 attaatctcc cgggctagtt ctcccagtcc ggttcctaaa atgatagcgg tattgggaac
     3901 atcaccgatt ctatttttta gataatcggc tgtttgattg atagcttcta acatgtttat
     3961 tgattttttt attaaatgat tctttttcgt ttttttctaa aaatactact tctatgggga
     4021 tatagtacaa cctttttttc tccctttcgt caagaaacga caatgcacga aggcgtacag
     4081 catctttctc ggtagttacg atcatcttat cgtcgtcgtc cacatccagg gtgttcattt
     4141 cccgtacaat ctcctcgata tcctcttctt taaaaaaatg atgatcggga aagtatttcg
     4201 gatataaatt gtacgttttc agttccagct tttctactaa cggctgaggt gatgcaattc
     4261 cggtaatcag aaaaacgtgc tttttgcgca aatcatcggg ctcgagaatt tccggttgca
     4321 actcggggaa aacaggtatg aggctaccgt aatcaaaagt ggtgaagtac agatcctgat
     4381 aaggaaataa atcgaggccg ttttcataaa tacggaagtc gatgggttgc atatcgcggc
     4441 tgcatttggt gacaatgacg attgaagcgc ggtcttttcc ctgtaacggc tctcgcaagt
     4501 ttcctgcggg aagtaatttg tcctcgtaga ccgggcggtt actgtcgacc aataagatgg
     4561 agagagacgg agtgacgtag cggtgttgga aaccgtcgtc cagcagaatg acctgaggtc
     4621 gctcttctat gtcgatggat tgtattttct ctatggcgct tctcctgttt ttatccacc
//
LOCUS       DAOSDR010000258         4629 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrimonas sp. isolate AMR_MDS_1826
            NODE_6538_length_4629_cov_3.780280, whole genome shotgun sequence.
ACCESSION   DAOSDR010000258 DAOSDR010000000
VERSION     DAOSDR010000258.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863355
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrimonas sp. (wastewater metagenome)
  ORGANISM  Petrimonas sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Dysgonomonadaceae; Petrimonas.
REFERENCE   1  (bases 1 to 4629)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4629)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:10:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,720
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 35
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4629
                     /organism="Petrimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6538_length_4629_cov_3.780280"
                     /isolate="AMR_MDS_1826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2023866"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..180
                     /locus_tag="PLI30_09945"
     CDS             <1..180
                     /locus_tag="PLI30_09945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006801277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI79912.1"
                     /translation="TPGYRRFIQSNNLIVWSKASVTAVEMGSNLFPEEVIEAGEWGKI
                     SVLCRQGLAIIRNNR"
     gene            212..1669
                     /locus_tag="PLI30_09950"
     CDS             212..1669
                     /locus_tag="PLI30_09950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009132635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOI79913.1"
                     /translation="MSNLQTGKMTNHRWTIVVMLFMATTVNYLDRQVLSLTWADFITP
                     EFHWTNSDYGTITGLFSIFYAVSMLFAGKFIDWMDTKKGFLWAIGVWSFGAVMHAFCG
                     LLTAGITAGEWTMSFHGARQAIGTVGDVSLVVSVSVTLFVFARLVLAIGEAGNFPAAI
                     KATAEYFPKKDRAYATSIFNSGAQIGALLAPLTIPFIAKAWGWEMAFIVIGALGFIWM
                     GFWVFIYHKPEKSKKVNTLELAYINQDDATDATEGRVKADENAGKKVSFNKAFKYKQT
                     WSFAVGKFLTDGVWWFLLFWIPAYLSSVYGLDSTQSAPHVFVVYAISMISVFAAGYFP
                     AYFMEKKKLDPYQGRMRAMLLFAFFPLLILLAQPLGGFSVWLPVIIIGIAAAAHQSWS
                     ANIFTTVSDMFPKHAVGTITGIGGMAGGVGSFFINKSSGVLFDYAANTNLKFLGYEGI
                     ESGYFIIFIFCAVAYLLGWTIMKSLVPKYQLITDM"
     gene            complement(1679..3058)
                     /locus_tag="PLI30_09955"
     CDS             complement(1679..3058)
                     /locus_tag="PLI30_09955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019539159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOI79914.1"
                     /translation="MSWSKKQLSTLITVSITSFMGTFLISSINIALPAIEKSFALNAV
                     SLSWIITGFLVATGMFLLPVGKLGDISGNVRLFKLGLIVFTLASMLCAISPSGIWLIA
                     ARFVQGVGSAFTSTTGQAILVANFPARNRGQVIGISVASVYTGLATGPFIGGFLTQAL
                     GWHSLFYVAAVLGILSTAIAFSFLEKDVAVHGGEKKIDLRGTFVFMPGLIALVYGSSH
                     IPSSSGWALMAFGIILLALFWIIESRVVYPMFETRLFTHNKLFGFSNLAALINYTATS
                     AIVFFLSLYLQKIQGLSPRDAGAVIIAQPVMMALFSPVVGRLSDKIQPRYFATLGMAM
                     CSAGLFAMSFFTAATPLWLIVSILIWEGLGFAFFSSPNMNTIMSSVDKSRYGQASGTA
                     SSMRIFGQIAGMTIVTFFFAFYFGSNTVTEVTDTVFLTAMKWGFITFTLISLVGIYFS
                     FTRGNVERQ"
     gene            complement(3063..3581)
                     /locus_tag="PLI30_09960"
     CDS             complement(3063..3581)
                     /locus_tag="PLI30_09960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004291294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HOI79915.1"
                     /translation="MTIIKSVRGLTPEIGDNTFLAETAVVIGDVVMGKDCSIWYGAVL
                     RGDVNSIRLGDRVNVQDGAVIHTLYEKSVSILGSDVSVGHNAIIHGATIENGALIGMG
                     AIILDHAVVGEGAIIAAGALVLSGTQVEPGSIYAGVPAKFVKKVDPGQSKEMNQKIAA
                     NYLMYSGWYKEL"
     gene            complement(3645..>4629)
                     /locus_tag="PLI30_09965"
     CDS             complement(3645..>4629)
                     /locus_tag="PLI30_09965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019538760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aminopeptidase family protein P"
                     /protein_id="HOI79916.1"
                     /translation="AVCLTGHKINYKLYQTLSATCRIIDVPSPVDLMKAVKNETELNG
                     FRHAMVKDGVALVKFFMWLEKAVPEGSVTEITVDEKLREFRSQQELFVGESFSTIAGY
                     ARNGAVIHYRAQPDTCLEIEPKGLLLIDSGGQYKDGTTDITRTVAVGKLTKQMREDYT
                     NVLKGHIGIATVVYPEGTRGSQLDVLARKALWNNCQTYWHGTGHGIGHFLNVHEGPQN
                     IRLEENPTVLKVGMVTSNEPGIYRTNEYGIRIENLIVTQEYNKTEDFGTFYNFETITL
                     CPIDTKPIVKKLLTKKEIKWLNSYHEMVYSKLKKHLNKTEKEWLKEKTRPI"
BASE COUNT         1232 a         1171 c         1041 g         1185 t
ORIGIN      
        1 acccccggat accggcgatt tattcaatcg aataacctca tcgtctggtc caaggccagt
       61 gtcaccgccg tggaaatggg ttccaacctc ttcccggaag aagtgatcga agcaggcgag
      121 tggggaaaaa tctccgtatt atgccgccag ggactggcca tcattcggaa caacagataa
      181 tcattcatta acttaataac agaaataaat catgagtaat ctacaaacag gaaagatgac
      241 caatcaccgg tggaccatcg ttgtgatgct gtttatggcc accacagtga actacctcga
      301 caggcaggta ctgtcactga cttgggccga ctttatcaca ccggagttcc actggaccaa
      361 cagtgactac ggtaccatca cggggctctt ctccattttt tatgccgtat cgatgctctt
      421 cgccggtaaa tttatcgact ggatggatac caagaagggt tttctctggg ccatcggtgt
      481 atggtcgttc ggtgcggtga tgcacgcttt ctgcggcctc ttgactgccg gtattactgc
      541 aggcgagtgg accatgagct tccacggtgc acgtcaggct atcggcacag taggagacgt
      601 atcgctggtg gtttccgtga gtgtcaccct cttcgtgttt gcgcgactgg tactggccat
      661 cggcgaggca ggcaacttcc ccgccgccat caaggccacc gccgagtact tccccaagaa
      721 agacagggct tatgccacca gtatcttcaa ctcgggtgca cagataggtg ctctgttggc
      781 ccccctcacc attcctttca ttgcaaaagc atggggctgg gaaatggcct tcatcgtgat
      841 cggtgccctt ggcttcatat ggatgggctt ctgggtattt atttaccaca aaccggaaaa
      901 gagtaaaaaa gtgaataccc tcgagttggc ctatatcaac caggatgatg cgaccgacgc
      961 tacagaagga agggtgaagg ccgatgagaa tgccggcaag aaagtctcct tcaacaaagc
     1021 attcaaatac aaacaaacct ggtcgttcgc tgtgggtaaa ttccttactg atggagtatg
     1081 gtggttcctg ctgttctgga taccggcata cctcagttca gtttacggtc ttgactccac
     1141 tcagagcgca ccccacgtat ttgtggtgta tgccatctcc atgatatcgg tttttgcagc
     1201 cggctatttc ccggcctatt tcatggaaaa gaaaaagctg gacccttatc aggggcgcat
     1261 gcgtgccatg ttgctcttcg cctttttccc cctgctgatc ctgctggcac agccactggg
     1321 aggtttctcg gtctggcttc cggtaatcat tatcggtatc gctgctgccg cacaccagtc
     1381 ctggtcggcc aacatcttca ccaccgtgag tgacatgttt ccgaaacatg cggtgggcac
     1441 catcaccggt atcggtggta tggcaggagg tgtgggttcc ttcttcatca acaagagctc
     1501 cggcgtactg ttcgattatg cagccaacac caacttgaaa ttcctgggtt atgaaggaat
     1561 tgaatcggga tatttcatca tcttcatctt ctgtgccgta gcctatctct taggatggac
     1621 catcatgaag tcattggttc cgaagtatca actgatcaca gatatgtaac agaaggggtt
     1681 attgtctctc cacattgcct cgtgtaaatg aaaaataaat tcccaccagg ctgatcagag
     1741 taaaggttat gaaaccccat ttcatggcag taagaaaaac agtgtcggta acctctgtca
     1801 cggtgttgct tccaaaatag aaagcaaaaa agaacgtgac gatagtcatt cctgcaattt
     1861 gtccaaaaat acgcatcgaa gatgctgtcc ctgatgcctg accgtaacgg ctcttatcca
     1921 ctgaactcat aatggtattc atattgggcg atgagaaaaa ggcaaagcca agcccttccc
     1981 aaatcaatat cgaaacaatt aaccataacg gagttgcagc agtgaaaaaa gacatggcga
     2041 ataatccagc cgaacacatt gccatcccta gcgttgcaaa gtaacgcggc tggattttgt
     2101 cggaaaggcg gcccactacg ggcgagaaca gtgccatcat aaccggttgg gctataatga
     2161 ccgcacccgc atcacggggt gacagcccct ggattttttg cagatagagg ctcaggaaaa
     2221 acacaatggc agaggtagcc gtataattga ttagagccgc caggttagaa aagccaaaaa
     2281 gcttattgtg cgtaaaaaga cgtgtctcaa acatagggta aaccacccgg ctctcaatta
     2341 tccagaacag ggccaacagg atgataccaa aagccataag agcccaaccg gaactggagg
     2401 ggatatgcga tgaaccgtaa actaacgcta ttaaacccgg cataaacaca aacgtccccc
     2461 gtaaatcaat tttcttttcg cctccatgaa cagccacatc tttttccaga aaagaaaaag
     2521 ctatggccgt agacaatatt cccagaacag ctgccacata gaacaacgaa tgccagccca
     2581 aggcctgtgt taaaaatcct ccgataaacg gacctgttgc caacccggtg taaaccgacg
     2641 ccactgaaat acctatcacc tgtccacggt tacgagcagg gaaattagct accaggatag
     2701 cctgtccggt ggtactggta aaagccgacc ctacaccttg tacaaaacgg gcagcgataa
     2761 gccagatgcc tgaaggcgaa attgcgcata acatcgacgc aagcgtaaaa acgatcagtc
     2821 ccaacttaaa gagtcgtaca ttgcccgaaa tatcgcccag cttacctact ggcagaagaa
     2881 acataccggt ggccactaaa aagccggtaa tgatccagct gagtgaaacg gcattaagcg
     2941 cgaaactttt ttcaatagca ggcaaggcaa tattaatgga tgatatcaga aacgttccca
     3001 taaacgaggt aatggaaacg gtaatcaacg tggacaattg tttttttgac caggacatgt
     3061 gtttataact ctttatacca ccccgaatac atcaagtaat tagctgctat tttttgattc
     3121 atctctttcg attgtccggg atctactttc tttacaaatt ttgccgggac acccgcataa
     3181 atgcttccgg gctccacttg cgtgccgctc aagacaagtg cgccggctgc tataatggca
     3241 ccctcaccca ccacggcatg atcaaggata atagctccca tcccgattag tgcaccgttt
     3301 tcgattgttg cgccgtgaat gatagcgtta tgtccaaccg acacatcgct gccaaggata
     3361 gaaaccgatt tttcataaag cgtatgaata accgctccat cctgtacatt cacacgatcc
     3421 cccaggcgaa tggaattgac atcgccccgt aacacggctc cgtaccagat actacaatcc
     3481 ttgcccatca caacgtcacc gataacaacc gccgtttctg ctaaaaaagt attatcgcca
     3541 atttccgggg tgagtccgcg aactgatttt attattgtca tattaaatgt atgggttatt
     3601 aactttcaac ttccgatctt aactttcaaa ccaccaaccc caatttaaat aggccgtgtc
     3661 ttttccttca accactcttt ttctgtttta ttcaagtgtt tctttaactt actgtatacc
     3721 atttcgtggt atgagttgag ccacttgatc tctttttttg tcagtaattt cttcactatg
     3781 ggtttggtgt caatcgggca aagcgtgatg gtttcaaaat tatagaatgt accgaaatct
     3841 tctgttttat tatattcttg tgtaacaata aggttctcga tccggatccc gtattcgttg
     3901 gtgcgataaa tgcccggttc attggaggtg accataccca ccttcaaaac cgttgggttt
     3961 tcctcgagac gaatgttctg gggcccttcg tgcacattga gaaaatgtcc gatgccatgt
     4021 cctgttccgt gccagtaggt ctggcaatta ttccacaacg ctttacgagc gagaacgtcg
     4081 agttgcgaac cccgcgttcc ttcggggtaa actaccgttg caataccgat atgtcctttc
     4141 agtacgttgg tgtaatcctc cctcatttgt ttagtaagtt tacccacggc taccgtacgg
     4201 gtgatatcgg tggtaccgtc cttgtattgg ccaccggaat cgatcagcaa caaacctttt
     4261 ggctctattt caaggcaagt gtcgggttgg gcgcgataat ggataacggc tccgttccgg
     4321 gcatatcccg ctatggttga aaagctctca cccacaaaca attcctgttg tgaacgaaat
     4381 tcacgtaact tttcatctac cgtaatttcc gttacgcttc cttcgggcac cgctttttcg
     4441 agccacataa agaacttcac caaagccacc ccgtctttaa ccatggcatg gcggaatccg
     4501 ttgagttcag tttcattctt aacggctttc atcaagtcca ccggcgaggg aacgtcgatg
     4561 atcctgcacg ttgccgaaag ggtctggtac aacttataat tgattttatg gccggtaagg
     4621 caaacggca
//
LOCUS       DAOSDR010000259         4625 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrimonas sp. isolate AMR_MDS_1826
            NODE_6544_length_4625_cov_3.694311, whole genome shotgun sequence.
ACCESSION   DAOSDR010000259 DAOSDR010000000
VERSION     DAOSDR010000259.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863355
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrimonas sp. (wastewater metagenome)
  ORGANISM  Petrimonas sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Dysgonomonadaceae; Petrimonas.
REFERENCE   1  (bases 1 to 4625)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4625)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:10:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,720
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 35
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4625
                     /organism="Petrimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6544_length_4625_cov_3.694311"
                     /isolate="AMR_MDS_1826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2023866"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..681
                     /locus_tag="PLI30_09970"
     CDS             <1..681
                     /locus_tag="PLI30_09970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HOI79917.1"
                     /translation="QRLATLSAESIENRYRERWENAVVTITPPEATYSDIGITGHFLR
                     QLPGGSALHLANSQAVYLTQQFNLPAGVDLFCNRGTNGIEGSLSTAVGYAAASERLTF
                     LLTGDMSFFYDMNGLWNRHLSPHLRILLNNNGGGGIFHTLPGLNRSEALDGFVAAAHT
                     TEARTWAEQRGFLCLTARNTEELQQQLPLFMNHKGDRPVLLEVFTSMEENSKHIASYY
                     QQLKNNRK"
     gene            678..1499
                     /gene="menB"
                     /locus_tag="PLI30_09975"
     CDS             678..1499
                     /gene="menB"
                     /locus_tag="PLI30_09975"
                     /EC_number="4.1.3.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009126288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008935 - 1,4-dihydroxy-2-naphthoyl-CoA
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA synthase"
                     /protein_id="HOI79918.1"
                     /translation="MIRREWITIKEYEDILFDLYNGIARITINRPRYRNAFTPTTTGE
                     MSDALRLCREMEEVSVVVLTGAGDKAFCSGGDQNVKGKGGYIGKDGVPRLNVLDVQKQ
                     IRSIPKPVIAMVNGYAIGGGHVLHVVCDLTIASENAVFGQTGPRVGSFDAGFGSSYLA
                     RIVGQKKAREIWFLCRQYNAQEALDMGLVNKIVPFDRLEDEVVEWAETMMQHSPLALR
                     MIKAGLNAELDGQAGIQELAGDATMLYYLTEEAQEGKQAFLEKRKPDFKKFPKLP"
     gene            1512..2576
                     /locus_tag="PLI30_09980"
     CDS             1512..2576
                     /locus_tag="PLI30_09980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005816442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate synthase"
                     /protein_id="HOI79919.1"
                     /translation="MFTTEIIPHTLRFKQPAGTSRGVYREHRVWYVLFRNNNDPSHMG
                     IGECAPLPDLSCDYDDNYAETLSRFCRITEEQQQVETELLRRYPSILFGLETALRHYL
                     QHSWQLWNSPFSRGEEGIPINGLIWMGSFDRMKQQAKNLLEKGFRCIKLKIGAIDFDQ
                     ELELLRYIRISHPSLTLRVDANGGFAPQEAGEKLQRLAPLKIHSIEQPIRAGQWEEMA
                     RLCNATPLPIALDEELIGIHYPDEKRRLLETIQPQYIILKPSLHGGISGCNEWIALAR
                     KLGIGWWITSALESNIGLNSIAQWCATLGNPLPQGLGTGSLYLNNLPLPLEIRDDSLW
                     FKTSGNFPDKPLQEYGKHND"
     gene            2557..3642
                     /locus_tag="PLI30_09985"
     CDS             2557..3642
                     /locus_tag="PLI30_09985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019537978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HOI79920.1"
                     /translation="MESITIEGIRYPREELVSETPPRFATRSPFHHKLSLFLREWYSP
                     SLLLTLHTSGSTGKPKEIIVRKDQMLQSAAITCDYLGLKTGDTALLCLPLEYIAGKMM
                     VVRALYGGFNLLVADPSGHPLTEDRQQINFAAMVPLQVYNSLQRKEEKERLTRIDRLI
                     IGGGAIDPQLEEALKDLPNAIYSTYGMTETVSHIALRRISGKEADDRYTPLPGVQVKL
                     SAEGTLVINAPRVADKPLTTNDIAEIGTTGTFRILGRKDNVVISGGIKIQIEAVEATL
                     RPHIDGPVVFTSVPHPKLGEALVLLITPASAIARAREICSRHLPRLQQPLHILMVEAI
                     PQTGNGKTDRAAAKRMASKRIPMPGIS"
     gene            complement(3639..>4625)
                     /locus_tag="PLI30_09990"
     CDS             complement(3639..>4625)
                     /locus_tag="PLI30_09990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005863595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HOI79921.1"
                     /translation="SSGESLHKRGYRVAQTEAPLNEVLAAGMILKSGWRGESTFVDPM
                     CGSGTLLIEAAMIALGIPPGIHRQSFGFEKWSDFDEDLFSDIYNDDSGSRDFPYRIVG
                     SDISPKAISAAEKNIRNAGLKKQIDLSVKPFQQYVEPPAPKGVLITNPPYGERIKVDE
                     LTELYAMIGERLKHVFTGYDAYVLSYRKECFDSIGLKHSKRFFLFNGALECEMRGYEI
                     FSGKRDDRPKKEGYRHSRTAGEGNEFKPKGEFKSKREYDKPEKSFTPSRRPSGTESKT
                     GRPLFERDDRPARHMPERRSSGTESRSGKRTYGDDKKPRSFNSSRGEKGRKS"
BASE COUNT         1154 a         1294 c         1183 g          994 t
ORIGIN      
        1 caacggctgg caacattgtc ggccgaaagc atagaaaacc gctaccggga acgctgggag
       61 aacgccgtag tgacgatcac acctccggaa gcgacctact ccgacatcgg cataaccggt
      121 cacttcctac gacaactacc gggaggatct gccctacacc tagccaacag ccaggccgtc
      181 tacctgacgc agcagtttaa cctcccagcc ggtgttgacc tcttctgcaa ccgaggcacc
      241 aacggtatcg agggttcgct ctctactgcc gtgggttatg ccgccgcatc ggaaaggctc
      301 acctttctat tgacaggtga tatgagtttt ttttatgata tgaatggact ctggaaccgg
      361 catttatcac cccacctgcg catcttgctg aacaacaacg gtggaggagg tatcttccac
      421 accttgcccg gactcaaccg gtcagaagcg ttggatggtt ttgtggctgc tgcgcacact
      481 acagaggccc gaacatgggc agagcagagg ggcttccttt gcctcacggc ccgcaacaca
      541 gaagagttgc agcaacaatt gccactcttc atgaaccata agggagaccg gcctgttcta
      601 ctggaggtgt tcacctctat ggaggagaac agcaaacata tcgcatccta ttatcaacaa
      661 cttaaaaaca acagaaaatg atacggagag aatggattac catcaaagaa tacgaagata
      721 tcctctttga tctttacaac ggaattgcac gaatcaccat caaccggccg cgataccgca
      781 acgccttcac cccaaccacg accggagaga tgagcgacgc cctgcgcctt tgtcgtgaga
      841 tggaagaggt aagtgtagtg gtgctcaccg gtgcgggtga caaagccttc tgctcgggtg
      901 gcgaccagaa cgtaaagggt aagggaggct atatcggcaa ggacggtgtt ccacgcctca
      961 acgtgctgga tgtacagaaa cagattcgct ccattcccaa gccggtgatc gccatggtaa
     1021 acggctacgc catcggcgga ggacatgtac tgcacgtagt gtgcgacctg actatcgctt
     1081 ccgagaacgc cgtcttcgga cagacgggcc cccgcgtggg tagcttcgac gccggtttcg
     1141 gatcttcata cctggcccgc atcgtgggac agaaaaaagc acgggagata tggtttctct
     1201 gccgccagta caacgcacag gaggcgctcg atatgggact ggtgaacaag atagtgcctt
     1261 tcgaccgcct cgaggatgag gtggtggaat gggccgagac aatgatgcaa cacagccccc
     1321 tggcgctgcg catgatcaag gccgggctca acgccgaact ggacggacag gcaggcatcc
     1381 aggagctggc gggagacgcc accatgctct actacctgac cgaggaggca caggaaggga
     1441 aacaagcgtt cctggaaaaa cggaaacccg atttcaaaaa gtttcccaaa ctaccctgat
     1501 ccgtttacac cgtgtttact accgagatca tccctcacac acttcggttc aaacaaccag
     1561 ccggcacttc acgcggcgta taccgcgagc accgggtctg gtatgtgctg tttcgtaaca
     1621 acaatgaccc ttcccacatg ggtatcggcg agtgtgcacc gttaccggac ctgagttgcg
     1681 actatgacga caactacgca gagacactca gccgcttctg tcggatcacg gaggaacagc
     1741 agcaggtgga aactgaactg ctgcgccgct acccctccat cctcttcgga ctggagacag
     1801 ccctacggca ttacctgcaa cattcatggc aactgtggaa tagccctttc agccgcggcg
     1861 aggagggtat tcccatcaac ggactgatct ggatgggaag ctttgacagg atgaagcagc
     1921 aggctaagaa cctgcttgag aaggggttcc gctgcatcaa gctgaagatt ggggccatcg
     1981 acttcgatca ggagcttgaa ttgcttcgtt atatccgtat cagccatccc agtctaaccc
     2041 tccgagtgga tgccaacggt ggatttgcac cccaggaggc aggggagaaa ctgcaaaggc
     2101 tggctccatt gaaaattcac tccatcgaac agcccatccg ggcggggcag tgggaggaga
     2161 tggcccggct ctgtaatgcc acacccctgc ccatcgcgtt ggatgaggag ctcatcggta
     2221 tccattatcc ggatgagaag aggagactgc tggaaacgat acaaccgcag tatatcatcc
     2281 tgaaaccctc actgcatggc ggcatcagcg gctgcaacga atggatcgca ctggctcgaa
     2341 agctgggaat cggctggtgg attacctccg ccctggaatc caatatcggg ctcaacagca
     2401 tcgcccagtg gtgcgccacc ctcggcaacc ccctgcctca gggactcggc accggctcgc
     2461 tatacctcaa caacctcccc ctaccattgg agatcaggga cgacagtcta tggtttaaaa
     2521 cgtcagggaa ctttcccgac aaacccctgc aggagtatgg aaagcataac gattgagggg
     2581 atccgctatc cccgggaaga gttagttagt gaaactcctc cgaggtttgc cacacggtcg
     2641 cccttccacc ataaactctc gctttttctg agagagtggt actctccctc tctactgctc
     2701 acgttgcata catcaggctc caccggtaaa ccgaaagaga taatagtacg taaggatcag
     2761 atgctgcaga gcgcggcgat cacctgcgat tacctgggat tgaaaacggg tgacacggca
     2821 ctgctctgcc tgccgttgga gtacattgcc gggaagatga tggtggtgcg cgcgctctac
     2881 ggtggtttca atttgttggt agcagatccg tccggccatc cgcttacgga agacaggcaa
     2941 cagatcaact ttgccgcaat ggttccactg caggtataca actccctgca aaggaaagag
     3001 gagaaagaac ggctcacaag aatcgacagg ctgattattg gcggaggggc tatcgacccg
     3061 caactggaag aagcgctgaa agatctcccc aacgccatct actccaccta cggaatgacc
     3121 gagacggtat cacacattgc gttgcgcaga atcagcggca aagaggccga cgatcggtat
     3181 actcctctac cgggcgttca ggtgaaactg tcggccgaag gaacgctggt gatcaatgcc
     3241 ccgcgggtag ccgataagcc actaactacc aacgatatcg cggagatagg gacaacggga
     3301 actttccgca tcctgggtag aaaagacaat gtagtcatca gcggaggtat caaaatacag
     3361 atagaagcgg tagaagcgac attgcgtcca catatagacg ggccggttgt atttacctcc
     3421 gtaccacacc cgaagctggg cgaggcattg gtgttgctga ttacaccagc atcagctatt
     3481 gcccgggcca gggagatatg ctcgcgccac ttaccccgct tgcaacaacc gttgcatatt
     3541 cttatggtgg aggcaattcc acaaaccgga aacggaaaaa ctgatcgggc tgcagcaaaa
     3601 agaatggcat cgaaacggat tccaatgcca ggtatatctt aggatttcct ccctttctca
     3661 ccgcgggaag aattaaaact ccgaggtttt ttatcgtctc catacgtacg tttcccgctt
     3721 ctgctttcag taccggaaga acgtctctct ggcatatgtc tagccggcct atcatccctc
     3781 tcaaagaggg gtcttccggt tttgctttct gttccggagg gacgcctgct cggggtgaat
     3841 gatttctccg gtttatcgta ctcacgtttg gacttaaact cgccttttgg tttgaattca
     3901 ttcccttccc cggcagtcct ggaatggcgg tatccctctt ttttaggacg atcatccctt
     3961 tttcctgaaa aaatttcata tccccgcatt tcacattcca gcgcaccgtt aaaaagaaaa
     4021 aaacgtttgg aatgtttcaa cccgatactg tcgaagcatt ctttccgata actcagcaca
     4081 taggcgtcgt atccggtaaa gacatgcttc agccgctccc caatcatggc gtacagttcc
     4141 gtaagctcat ccacctttat cctttccccg tacggcggat tcgtgatcaa aactcctttc
     4201 ggggcaggtg gctccacata ctgctgaaaa ggctttacac ttagatcaat ctgtttcttc
     4261 aatccggcgt tccttatatt tttttctgca gcggaaatcg ctttaggtga aatatccgaa
     4321 cccacaatcc tgtacggaaa gtcacgcgaa ccactatcgt cgttgtaaat gtcactgaaa
     4381 aggtcttcat cgaagtcgct ccacttttcg aacccgaaac tctgccggtg gatgccggga
     4441 ggaattccca aagcgatcat ggccgcctca atcaacaatg tacccgatcc gcacatgggg
     4501 tcaacgaaag tgctttctcc gcgccagccc gacttgagaa tcatgccggc agccagcacc
     4561 tcattcagag gcgcttcggt ttgcgcaacc ctatagcccc gtttgtgcag cgattctccc
     4621 gaact
//
LOCUS       DAOSDR010000260         4598 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrimonas sp. isolate AMR_MDS_1826
            NODE_6609_length_4598_cov_4.087827, whole genome shotgun sequence.
ACCESSION   DAOSDR010000260 DAOSDR010000000
VERSION     DAOSDR010000260.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863355
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrimonas sp. (wastewater metagenome)
  ORGANISM  Petrimonas sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Dysgonomonadaceae; Petrimonas.
REFERENCE   1  (bases 1 to 4598)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4598)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:10:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,720
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 35
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4598
                     /organism="Petrimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6609_length_4598_cov_4.087827"
                     /isolate="AMR_MDS_1826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2023866"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..329)
                     /locus_tag="PLI30_09995"
     CDS             complement(<1..329)
                     /locus_tag="PLI30_09995"
                     /inference="COORDINATES: protein motif:HMM:NF012787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin family protein"
                     /protein_id="HOI79922.1"
                     /translation="MSSSIKTEKTSTGYYPGEQIPNIVLSGLEGQHLDLSEYKGRKVV
                     LSFWAAYDAQSRASNVQLYNYLKEAAPDVTFLSISFDENRNVSERTALLDKIDLNSQF
                     CDETGASS"
     gene            756..1433
                     /locus_tag="PLI30_10000"
     CDS             756..1433
                     /locus_tag="PLI30_10000"
                     /inference="COORDINATES: protein motif:HMM:NF018178.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1080 domain-containing protein"
                     /protein_id="HOI79923.1"
                     /translation="MQIKNSLYKRTTPYIVVAFLIGLSACVGNQKSKNATETIPQRVD
                     SGWVQLFDGKSLDGWQITNFGTQGTVQVIEGEIVLGMGDGCTGITWNGNFPKTDYEVK
                     LEAKKITGNDFFCGMTFPVKDSFCTLIIGGWGGPVVGLSNIDGSDASDNESHTLKKFD
                     HDTWYAIHLKVSSEKIEAWIDGEQVVDFSTSGKQLSVRSEVSLSKPFGICSWRTTSAL
                     RNIHLKE"
     gene            1822..2346
                     /locus_tag="PLI30_10005"
     CDS             1822..2346
                     /locus_tag="PLI30_10005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase"
                     /protein_id="HOI79924.1"
                     /translation="MDNRKYNRRNFLKISGLTTMGSLLTHRLPTNNKHIYSVLNQGTI
                     PADTPQNRIAEIVETKLVSKESGKYLIIGQSIDENGHPKATQPFIEPNRCLGWPSITK
                     TDKGELLIVYSGDRDSHVCPWGKTRLIRSTNNGKTWSEPETVNNSPLDDQETYSINRK
                     IKGDLHLSSQHTGN"
     gene            2375..3250
                     /locus_tag="PLI30_10010"
     CDS             2375..3250
                     /locus_tag="PLI30_10010"
                     /inference="COORDINATES: protein motif:HMM:NF014708.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HOI79925.1"
                     /translation="MSNIGLIIEERAASIGNFMVGRLLPFRQKRTVGPFAFIDHMGPA
                     TMQSGENLDVPPHPHIGLSTLTYLFDGAIMHRDSIGNEVEITPGAVNWMTAGRGVVHS
                     ERTPERLRDTKKVLHGMQIWVALPTAMEETESSFFHIPAEEIPAWEMHGIQTKLIAGE
                     VFGKKSPVPVYSPLYLIELKSDKPQMFNPDGELYGESGLYILKGKVRIENYSFGPGKL
                     LIAQNSRLCAFEMGPDTRVYLFGGEPFPERRYIDWNFVSSRRERLLQAKEDWVNRRFP
                     EVPGESEFVPYPEFK"
     gene            3260..3862
                     /locus_tag="PLI30_10015"
     CDS             3260..3862
                     /locus_tag="PLI30_10015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008823434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HOI79926.1"
                     /translation="MKTLLIGATGATGSDLLQLLLNDGEIESVEIFVRRDPQIRHAKL
                     TVNRIDFERPEQWAHRVKGDILFSCLGTTLKDAGSKAAQWKVDFDYQYRFAKVAKENG
                     MKCFVLVSSMNASSRSPFFYARMKGKLEEAVRKLEFPKLLIFRPPSLIRKQSNRTLEL
                     AGVKAIRFFNRLGLFPSLRPLPTDQLAKEMLRAAKESVVG"
     gene            3894..>4598
                     /locus_tag="PLI30_10020"
     CDS             3894..>4598
                     /locus_tag="PLI30_10020"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="HOI79927.1"
                     /translation="MYKKFIFCWQLSLVVANALIIGLLISCLSIRDANRLSDYSPNTV
                     LKLPPSSNNPRNSEGDFITLKDGRVLFVYSHYTSGSGGDHDPAFLAGRYSADGGRTWT
                     TEDETIIENEGGMNVMSVSLLRLQNGNIALFYLKKNSTEDCIPMMRISRDEAKSWSDA
                     VPCITDKKGYFVLNNDRVIQLQDGRLLMAVALHQIPGGKWENQADLYCYFSDDNGQAW
                     QSSSQVPNNTDIITQEP"
BASE COUNT         1402 a         1001 c         1015 g         1180 t
ORIGIN      
        1 gagctggctc ctgtttcatc gcaaaactgc gaatttaaat caatcttgtc caagagcgcc
       61 gttctttcag agacgttccg gttctcgtca aacgagatcg atagaaacgt aacgtcaggg
      121 gctgcttctt ttaaataatt atacagctga acgttgctgg ctcttgattg ggcatcgtaa
      181 gccgcccaaa agcttaatac tacttttctg cctttatatt cgcttaagtc gagatgttgt
      241 ccttccaatc cactgagaac aatatttgga atctgttcgc cgggataata acctgtggaa
      301 gttttttcgg ttttgattga gctcgacata aagaaaagcg aaacaattgc tacaagacaa
      361 aagttcacat acttcataaa aaatgtttta attaatactc aaacttactt gtgcttcacg
      421 catttaacta agtttattta ctgttgactt ttcgctaaaa gtcatcattt ccagggacaa
      481 aattacgtcc gtttttatta aaaaaaaact tttacgccaa aaactttgtt tccaagtgtt
      541 aaaatcaact taaataaaca tacgataacc tatctatcag ggatatacaa acaatcaccc
      601 cgaatcgtat tgaggtaaat ttccacaccc agtaacgaac atcaacaaag taccaaaaaa
      661 caggaacgat ccctttcaat taaaaaataa ttataccttt gttccaattg ttaagtagcg
      721 gctataagtg cataaaaaaa gcaaaacatt tcttcatgca aataaaaaat tcactttaca
      781 agagaacaac cccttatatc gtagtagctt ttctgattgg tttatctgct tgtgttggca
      841 atcaaaaaag taagaacgcc actgagacta taccccaacg tgttgattcc ggctgggttc
      901 agttgtttga tggcaaatca ctggatggct ggcaaattac caactttggt acgcaaggca
      961 cggttcaggt tattgaaggt gaaattgtgc ttggaatggg agatggctgt acaggtatta
     1021 cctggaatgg gaatttccca aaaacagatt acgaagtgaa gctggaagct aaaaaaataa
     1081 ccggtaacga ttttttttgc ggaatgactt tccctgtgaa agattcgttt tgcactttga
     1141 tcataggagg atggggcggt ccggtggtgg gtcttagcaa catcgacgga tcagacgcat
     1201 cggacaacga aagtcacact ttgaaaaaat tcgaccacga tacctggtac gccattcact
     1261 taaaagtatc ctccgaaaag attgaagcat ggattgatgg ggaacaagtc gtagattttt
     1321 ctacctcggg aaagcaactt tccgtccgca gtgaagtaag cctttcaaag ccctttggca
     1381 tttgttcgtg gaggacaacc tcagctttgc gaaatattca cttgaaagag taagtaagta
     1441 ttagctactt tttaacagtc actttttatc gtcaacccca attgtctgaa gaacaaagct
     1501 tggtaaaact caactaccac cacagatcta gctgcagtgc aaaatattgt tccatatgca
     1561 tagaaccgat accggatggc tccaaaactt ggttctattt acttataatc gagttgataa
     1621 acttgttttg tatttcctcc ggtgtttgga tttcacaatg gtaagtccga tatttatttg
     1681 aaaattatgg tatttttgtt ttttattcga acgaaacatc acttaattta actttccatg
     1741 agtcaaacaa aattaatcag ctcatttcta aacagataca aacctctacg caattgaata
     1801 tttattcgat aacagaaaaa gatggacaac agaaagtaca acagacgcaa tttcttaaaa
     1861 atctccggat tgacaacaat gggaagccta ctgacccatc gattgccgac aaacaataag
     1921 catatttatt ccgttttgaa ccaaggaaca atacccgcgg acacaccaca aaacaggatc
     1981 gctgaaatcg tggaaacaaa gcttgtctcc aaagagtccg gaaagtacct gattattggt
     2041 cagtcaattg acgaaaacgg ccatccaaaa gcaacccaac cttttatcga acctaaccgc
     2101 tgcctcggct ggccttcgat cacaaaaacc gataagggag agttgctgat tgtttactcg
     2161 ggagacagag attcacacgt ttgcccctgg ggaaaaaccc gactcatccg cagtacgaat
     2221 aatgggaaaa cctggtctga accagaaaca gtaaacaaca gtccattgga tgaccaggag
     2281 acgtattcta tcaacaggaa aatcaagggg gacctacatc tgtcttcaca acacactgga
     2341 aattaaaaca aggtaattca taaaataaaa taatatgtca aacattggat tgatcattga
     2401 agaacgggcg gccagtatcg gtaactttat ggtaggacgt cttctgcctt tccgacagaa
     2461 aaggacggta ggccctttcg catttatcga ccatatgggg cctgccacga tgcagtccgg
     2521 agagaacctg gatgtaccgc cacatcccca tatcggcctt tccaccctca cctacctgtt
     2581 cgacggggca atcatgcacc gtgacagcat cggcaatgag gtggagatta cacccggggc
     2641 tgtgaactgg atgacagccg gtagaggagt ggtccactcg gagcgaacac ccgagcgact
     2701 gcgcgacacc aaaaaagtgc tccacggtat gcaaatatgg gtggcactcc ccacagcaat
     2761 ggaggagaca gaatcttcat tcttccacat ccccgctgaa gagatacccg cctgggagat
     2821 gcacgggata caaacaaagt tgatcgccgg tgaagtgttc gggaagaaat caccggtacc
     2881 ggtctacagc cctctatacc tgatcgagct gaaaagcgac aagccgcaga tgttcaatcc
     2941 ggacggtgaa ctgtatgggg agagcggact ctatatcctg aaagggaagg tgcgaattga
     3001 aaactacagt tttgggcccg gaaagttgct gattgcacaa aacagccgtc tctgcgcgtt
     3061 cgagatgggc cctgacacca gggtctacct ctttggcggc gaaccttttc cagaacggcg
     3121 ttatatagac tggaacttcg tctcttcgag aagggagaga cttctacagg caaaagagga
     3181 ttgggttaac cgtcgcttcc cggaagtgcc gggagagtca gaatttgtgc cttatccgga
     3241 atttaaatag agaaaattga tgaaaacact gttaatcggt gccacgggtg ctacgggcag
     3301 cgacctgctg cagctgttgc tgaacgacgg cgagatagag agcgtagaga ttttcgtcag
     3361 gagagacccg cagatacgac acgctaaact gacggtaaac cggatcgatt ttgaaaggcc
     3421 ggagcaatgg gcccaccggg tgaaggggga tatcctcttc tcttgcctgg ggaccaccct
     3481 gaaagacgcc ggcagtaagg cagcgcaatg gaaggtggat ttcgattacc agtaccggtt
     3541 tgctaaagtt gccaaagaaa acggaatgaa atgctttgtc ctggtctcat cgatgaacgc
     3601 ctcctcgaga tcaccgttct tttatgcccg gatgaaagga aaactggagg aagcagtgcg
     3661 gaaactggaa ttcccaaaac tattgatatt tcggcctcca tcactgatac gcaaacagag
     3721 caatcggacg ttggagctgg ccggggtgaa agcgattcgt ttcttcaacc ggctgggact
     3781 cttcccctcg ctgcgaccgc taccgacaga ccagctggcc aaagaaatgc tccgtgctgc
     3841 caaggagagt gtcgtcggat gattcagaca aaataaaaat ttatagtatt tttatgtata
     3901 aaaagttcat attttgctgg caattaagtc ttgttgttgc caacgcccta attattgggt
     3961 tactcatttc ttgcttatcc attagagatg caaacagatt atccgattat tctcctaata
     4021 cagtattaaa actacctccc tcaagcaata atccccggaa cagtgaaggc gattttataa
     4081 ctcttaaaga cggcagggtt ttgtttgtgt acagccacta caccagtgga tccggaggag
     4141 atcatgatcc ggcctttctg gcgggccgat attctgcaga tggaggaaga acctggacta
     4201 ctgaagatga aaccatcatt gagaatgaag gagggatgaa tgtgatgtca gtttcgttgc
     4261 ttcgtttaca aaacgggaac atagccctct tttacttgaa gaaaaactca acggaagact
     4321 gtattcccat gatgcgaatt tctagagatg aagccaaatc ctggagtgat gccgtaccct
     4381 gcatcactga taaaaaaggt tattttgtcc tcaataacga cagggtcatc caattacaag
     4441 atgggcgatt attgatggct gttgcattac atcaaattcc tggagggaaa tgggagaacc
     4501 aagctgattt atattgttac ttttcggatg ataatggaca agcctggcaa tctagctccc
     4561 aagttcctaa taataccgac attatcactc aagaaccc
//
LOCUS       DAOSDR010000261         4585 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrimonas sp. isolate AMR_MDS_1826
            NODE_6638_length_4585_cov_3.938190, whole genome shotgun sequence.
ACCESSION   DAOSDR010000261 DAOSDR010000000
VERSION     DAOSDR010000261.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863355
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrimonas sp. (wastewater metagenome)
  ORGANISM  Petrimonas sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Dysgonomonadaceae; Petrimonas.
REFERENCE   1  (bases 1 to 4585)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4585)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:10:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,720
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 35
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4585
                     /organism="Petrimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6638_length_4585_cov_3.938190"
                     /isolate="AMR_MDS_1826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2023866"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(143..544)
                     /locus_tag="PLI30_10025"
     CDS             complement(143..544)
                     /locus_tag="PLI30_10025"
                     /inference="COORDINATES: protein motif:HMM:NF025092.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HOI79928.1"
                     /translation="MIVLFFALSMRVSGQNSTLKGVVKDVDGIPLIGVNVVEKGTTNG
                     TVTDIDGNFSINVKTHSTVIFTYIGFAEQELVWDGNSVMNVVLREDSELLDEVVVVGY
                     GTQRKVNLTGAVSQVSNEVLENRPAPNLTRL"
     gene            complement(840..1745)
                     /locus_tag="PLI30_10030"
     CDS             complement(840..1745)
                     /locus_tag="PLI30_10030"
                     /inference="COORDINATES: protein motif:HMM:NF040125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6528 family protein"
                     /protein_id="HOI79929.1"
                     /translation="MTKCKCVDWIALVFFLFVAVSCSESNSKRPREIIVGGDNVVMIL
                     DFANSADSVKKVNWYLKTSEVTNMPDTMIRHMRTVDDHKSVDNHTKILICSSSGGTLL
                     VDRATRKCLFYAITPNAHSVEYLPGNRIAVALSTADKGNRLEIYDAGRSNTVLFSDSL
                     YSGHGVTWMAGRNLLYALGYDELRAYSLVDWDTPNPGLQLEEKWTLPETGGHDLYASS
                     ENQLLITTSKKVWKFDLNSNAFEPFAPLANEPDVKSVYYDEKADHLIYTQGEISWWTH
                     NIYSINPTKKIMIPEIDVYKVRVIK"
     gene            complement(1753..3522)
                     /locus_tag="PLI30_10035"
     CDS             complement(1753..3522)
                     /locus_tag="PLI30_10035"
                     /inference="COORDINATES: protein motif:HMM:NF019592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HOI79930.1"
                     /translation="MKTIKYLHLTVIITAIIFLYSCNDDFLELKPLTKLAESEAFWEN
                     ESSIETFSNSFYNYIDRGDILADFTSDNCEHKTSPPALRRGVYSMPTALGSGGWNWSQ
                     LRNINYFIQNVNNSKLDPSVKNKHIALGKFFRAWFYYKKVQQFGDVPWYSQPLATSDE
                     DLIFKPRDPRTLIVDSIRKDIDFAIRYLPVEKTKNRISKWMGLALKSRILLYEGTYSK
                     YHGLRDANACKVLLQEAVDASKELMESGMYTIYSTGDPHRDYFNLFQPKDAYTEEVIL
                     ARSTQNQYFYYTPNFTSTSNGNYGATYSLISDYLMSNGETFQKRYPDESKRRALPYMD
                     EFKDRDPRLWQTLVFPGYVRVGTSGEALTDFNQNTTGYMIHKRVGPPIEDQGGGLRDV
                     IMIRYAEVLLNYAEAKAELGILTQEDLDISVNLIRKRVNLPPLSINSPLDPYLDDYYK
                     NTSDPMILEIRRERRVELAFEGFRTEDLKRWKEGQLFRDRYQGMYIKEFNTYIDLNND
                     GSPDLYVLKNNQTPPSDRIAGVQYFRLADVAALSEGEYGRIIPYSRELPEFKDWEYLN
                     PIPTEELTLNKMLKQNPGWDNLN"
     gene            complement(3534..>4585)
                     /locus_tag="PLI30_10040"
     CDS             complement(3534..>4585)
                     /locus_tag="PLI30_10040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="SusC/RagA family TonB-linked outer membrane
                     protein"
                     /protein_id="HOI79931.1"
                     /translation="GLDFSLFNSKFNTTLDVFRRDTKDMLANYRSLPSVFGASVPKEN
                     IASLRTKGWELNVNWNDSFTLLDDRFMYGVYFNVSDYMSQITDYYNPTNYLADYYVGQ
                     TIGEIWGLTTLGYFLTDEEAKKSAVLSSSSTSRVYASAGTIKFEDKNNDGVISWGDRT
                     LDNPGDYRVIGNETPRYQFGLTLNGAWKGFDMNVFFNGVGKRDIYPGAEAVNFWGPYN
                     RKYQVMTKHVIENRWTPENPNAYFPKPQGYIALGGNDLGQPQTKYMQNAAYIRLKNFS
                     FGYSLPHDLVKKIKLNSLRFFVSGQNLWEYTKLHFSLDPEGLMKDPDANQGSVGLGTA
                     YPIQRVYSFGAQIQF"
BASE COUNT         1244 a         1070 c          883 g         1388 t
ORIGIN      
        1 ttggatcacc ctccactccg tctatcaaaa ccaacgcact tcctcctgcg ccaattgagg
       61 ttatacccct gatattgtaa tccgccgttc ttgtaggact tccatccgac atcctgatat
      121 tgaggtttgg caaggttccc tgctaaagcc ttgtcaggtt tggggcaggc ctattttcaa
      181 gcacctcatt acttacttga gaaacagcac ctgtcaggtt tactttcctc tgggttccat
      241 aacctacaac aaccacttca tccaacaact ccgaatcttc acgaagaaca acattcatca
      301 cggaatttcc atcccagaca agttcttgtt ccgcaaatcc aatataagtg aatataacgg
      361 tagagtgtgt cttgacattt attgaaaaat ttccatcaat atccgtcact gttccattcg
      421 tagtcccttt ctctaccaca ttaaccccga taagtggaat gccatccaca tcttttacaa
      481 ctcctttaag agtactgttt tgtcctgaaa ctctcataga aagagcaaaa aagagtacaa
      541 tcaaacaaat aaaagtgttt gataaattaa ctttaaccat aaaatttcta tctaattata
      601 ttaaaaactc ggcggcaaag gaaaggtatt attgttacga ggtggtttcg tttatattaa
      661 gagtttgtta attaacatat ggattagcct gcatggctaa cctcataaaa cagtaggtga
      721 attaccgttg gatgcaggct tcactatcac tatcctgcat gaacgtcaac acccgtaaga
      781 ggggttataa aaccgactta cgggtgcttt agtgcagtgc tgattaaaac ttatccggat
      841 tatttgataa ccctcacctt gtacacatca atttcgggga tcattatttt tttggttgga
      901 tttatggagt atatgttgtg ggtccaccag cttatctcac cctgggtata gatgagatga
      961 tccgcttttt cgtcgtaata gaccgacttg acatcgggct cgttcgcaag gggagcaaaa
     1021 ggctcaaacg cattgctgtt taaatcgaac ttccatactt ttttcgacgt ggttatcagc
     1081 agctgatttt ccgaagaggc atacaggtca tgcccgcccg tttccggcaa tgtccacttc
     1141 tcctccagtt gtaaacccgg gttgggagtg tcccagtcaa ccagcgaata cgccctcagt
     1201 tcatcgtagc ccaacgcata gagcaagttc cttcccgcca tccaggtaac gccgtggccg
     1261 gaatacaacg aatcgctgaa caacacggta tttgaacggc ccgcatcgta aatctccaac
     1321 cggttcccct tgtcggcggt agacaatgca accgctatac ggttaccggg aaggtattct
     1381 acggagtgcg cattgggtgt gatggcgtaa aagaggcatt tccgggtcgc acggtccacc
     1441 agaagggttc ctccgcttga ggaacagatc aggatcttgg tatggttgtc taccgatttg
     1501 tgatcatcaa cggtcctcat gtgccggatc attgtatcgg gcatgttcgt gacttcggag
     1561 gtcttcaggt accaattgac tttctttacg ctgtcggccg agttggcgaa atccagaatc
     1621 atcaccacat tgtctccccc gacgattatt tcccgcggac gcttcgagtt tgattcgctg
     1681 cacgaaacgg caacgaaaag gaagaaaacg agagcgatcc aatcaacaca tttacatttt
     1741 gtcattgttc ttttagttta agttatccca tccgggattt tgtttcaaca ttttatttaa
     1801 cgtaagctct tcggtaggaa tcgggttgag gtattcccag tctttgaatt cgggcaattc
     1861 tcttgagtaa ggtattattc tgccatattc cccctcactc aatgccgcca catccgccaa
     1921 acgaaaatac tgaactcctg cgatgcgatc ggacggagga gtctgattat ttttcaggac
     1981 atataagtcg ggactcccat cattattcag atcgatatag gtgttgaatt cttttatata
     2041 cataccttga taacgatccc tgaagagctg tccttctttc catcttttta aatcttccgt
     2101 tctaaatcct tcaaaagcca actccaccct cctctctctt cttatttcaa gaatcatggg
     2161 gtctgaagta tttttataat aatcatccaa ataaggatcc agaggcgaat ttatggaaag
     2221 aggaggtaaa tttactcttt ttctgattaa attcacagaa atatccaagt cttcttgtgt
     2281 taaaatgcct agctcagctt tcgcctccgc ataatttaat aaaacctctg catacctgat
     2341 catgatcaca tccctaagtc cgccgccctg atcttctata gggggcccta ctcttttatg
     2401 tatcatataa ccggtagtat tctgattgaa atcggtcaaa gcttcgccac ttgtccctac
     2461 ccttacgtat ccggggaaaa ccagggtttg ccatagacgg ggatctctgt ccttaaactc
     2521 atccatataa ggtaatgcac gcctcttgct ctcatcgggg tatcttttct gaaatgtctc
     2581 cccattactc atcaagtaat cagaaattaa agaataagta gcgccatagt ttccattcga
     2641 agtggatgtg aaattaggag tataataaaa gtattggttc tgtgtggacc gggctaaaat
     2701 aacctcctcc gtataagcat ccttgggctg gaaaagatta aagtaatcac gatgcggatc
     2761 tcccgtagaa taaatcgtat acattccgga ttccattaac tccttgcttg cgtcaacagc
     2821 ctcctgaaga agtaccttgc aggcatttgc gtccctaagc ccatgatatt ttgaatatgt
     2881 tccttcgtat aacagtatac gggacttcaa cgcaagtccc atccattttg aaattctgtt
     2941 ttttgttttt tcgacaggaa gataccggat ggcaaaatct atatcctttc ttattgaatc
     3001 aacaattaag gtacggggat ctctcggttt aaagatcaaa tcttcatcac ttgtcgctag
     3061 cggctgggaa taccagggga catctccgaa ttgctgcact ttcttataat agaaccatgc
     3121 gcggaaaaat tttcctaaag ctatgtgttt attttttaca gacggatcca gcttagagtt
     3181 attcacgttt tgaatgaaat agttgatatt ccttaattga gaccagttcc atccgccact
     3241 gccaagtgct gtaggcattg aataaacgcc cctgcgaaga gccggaggtg aagttttgtg
     3301 ttcgcaatta tcgcttgtga aatctgcaag aatatcgcct ctatcgatgt aattgtagaa
     3361 actgttcgaa aaagtttcaa tgctactttc attttcccag aatgcctcgg attccgccag
     3421 ttttgtcaat ggttttaatt ccaaaaaatc gtcgttgcag gaatacagaa atattattgc
     3481 tgtaattatt acggtgaggt gtaaatattt tattgttttc atattttcat cttttaaaat
     3541 tgaatttgtg ctccgaaaga ataaaccctc tggatggggt acgcagtacc caggcctaca
     3601 cttccctgat tggcgtccgg atccttcatc aacccttcag gatcgaggct gaaatgcagt
     3661 tttgtatatt cccagagatt ttgcccggaa acaaagaatc tcaggctgtt cagtttgatt
     3721 ttcttcacta gatcatgggg taaggaatag ccgaatgaaa agtttttcaa ccgtatatag
     3781 gcggcatttt gcatgtactt ggtttgaggt tgccccagat cattcccccc taatgcaata
     3841 tatccctgtg gtttgggaaa gtatgcattc ggattttcag gtgtccatct attttctatc
     3901 acatgctttg tcatcacctg atatttccgg ttataaggcc cccagaaatt aacggcttcg
     3961 gcgccgggat aaatgtccct tttcccaacg ccattaaaaa acacattcat gtcgaaccct
     4021 ttccaggcac cattaagcgt taatccaaac tgataacggg gagtctcatt ccctataacc
     4081 ctataatctc caggattatc caatgtcctg tctccccaac taatgacacc gtcgttgttc
     4141 ttatcttcaa actttatcgt tcccgcactg gcatagaccc tgctggttga gctcgacgac
     4201 aagaccgcac tctttttagc ctcttcatcc gtaagaaaat accccaacgt agtcaagccc
     4261 cagatttctc caatagtttg tcccacgtaa taatctgcca aataatttgt cggattataa
     4321 taatccgtta tttgggacat gtagtccgac acgttaaaat acacgccgta cataaacctg
     4381 tcatccagca gagtaaaaga atcgttccaa ttgacattca actcccatcc tttcgttctt
     4441 aaagatgcaa tgttttcttt agggacactt gctccgaaaa cagatggtaa tgacctgtag
     4501 ttggcaagca tatctttggt gtctctcctg aaaacatcca atgtcgtatt gaatttactg
     4561 ttgaacaaag aaaaatccag accta
//
LOCUS       DAOSDR010000262         4576 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrimonas sp. isolate AMR_MDS_1826
            NODE_6668_length_4576_cov_4.306348, whole genome shotgun sequence.
ACCESSION   DAOSDR010000262 DAOSDR010000000
VERSION     DAOSDR010000262.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863355
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrimonas sp. (wastewater metagenome)
  ORGANISM  Petrimonas sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Dysgonomonadaceae; Petrimonas.
REFERENCE   1  (bases 1 to 4576)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4576)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:10:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,720
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 35
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4576
                     /organism="Petrimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6668_length_4576_cov_4.306348"
                     /isolate="AMR_MDS_1826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2023866"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..520)
                     /locus_tag="PLI30_10045"
     CDS             complement(<1..520)
                     /locus_tag="PLI30_10045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008768011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOI79932.1"
                     /translation="MDKYSEEQLLEKLRDPRTQRQGFAILVSEYSERLYWQIRKMVLS
                     HEDTNDILQDVFIKAWINIDNFRGESKLTTWLYRIAINESITFLNKKRNQNNVSVDDE
                     NSFLLNTLEGDKYFDGDEAQKLLQQAILTLPDKQRLVFQMKYFDEMKYEDMSEILGTS
                     VGALKASYHHAVK"
     gene            complement(560..1648)
                     /locus_tag="PLI30_10050"
     CDS             complement(560..1648)
                     /locus_tag="PLI30_10050"
                     /inference="COORDINATES: protein motif:HMM:NF024579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOI79933.1"
                     /translation="MKKILIILFLISIVILNTNGQGNNVQTLIHEGVALHDSAEYKKA
                     IEKFEQALKINPKSTLALYEISLSYLELKDYENASKYSTRVINSNDKNLSVGAYAVKS
                     EAMAEMKQIDNAIAILQEGLIKNGDSYLLHFNMALNYYKKGDIDKTLEHVSRAIELDK
                     TNSGAFLLNAYAQRDKGLWVRSIYSFQLFLLLEPDSKRSKNAFEEMLQTMLVKPVTEK
                     PVERSFIQQQLLRNMPENSVQQEMPPLSTEEGLNRKIIYNAIKFSMDSLKAAKKDTDV
                     YFVFTEVNKAILSALEKESGALKSGSFWTFHYPFFKSILNSNHYDTFCRYISVSYFPE
                     SLEWWENNKTDAENFINWFENGEDNGKN"
     gene            1984..2820
                     /gene="prmA"
                     /locus_tag="PLI30_10055"
     CDS             1984..2820
                     /gene="prmA"
                     /locus_tag="PLI30_10055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006842805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008276 - protein methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006479 - protein methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HOI79934.1"
                     /translation="MKYVEVNFICNPDSEIITDVLAAQLSDIGFESFVKSNTGLLAYV
                     PEPTFSTEKIDTLLQNFFLDAEIGYSFKTIEDRNWNEEWEKHYFQPIVFENKCIIHSS
                     FHQPGGDYNYRILIDPKMAFGTGHHQTTGLILKEILSMDLQNKSVLDMGCGTAVLAIL
                     ASMCGADPIIAIDIDEWAYQNALENVHLNRINNISVLCGDSGLLGNDMFDVIFANINR
                     NILLQDIPAYTKVLNPGGKLVMSGFYREDIPVIHEKCVQYGLTYRDFSEMENWVAVVC
                     EK"
     gene            2918..>4576
                     /locus_tag="PLI30_10060"
     CDS             2918..>4576
                     /locus_tag="PLI30_10060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005640306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative transporter"
                     /protein_id="HOI79935.1"
                     /translation="MEWINDLIFGSGVAHAIFVLALVISSGILLGKIKVFGVSLGITW
                     ILFVGIFLGHLGLGIDEHILHFIKEFGLILFIYSIGMQVGPSFFSSFKQGGITLNLLA
                     SSVVFLGVITTYVIHVITGLPISTMVGILSGAVTNTPGLGAAQQTYFDVTGSQDPSIA
                     MGYAVAYPLGVVGIILSLILFKSLFRINFARENEAISITNASKMTEAQMFSLQVHNPA
                     VFGKTIHEIKSLMDKEFVISRVLHNDSGILTVPLPHTVLNENDKILVVTNLQNIELIE
                     ALIGQKIDMDRNEWNKLDNQLISRRINVTKAGINGKSIGQLRLRNLYGVNITRVNRAG
                     VDLVADPRLQLQLGDRVTVVGSEASIANVEKFLGNSLKRLREPNLISIFIGIALGVLI
                     GSIPFMFPGIPQPVKLGLAGGPLIVAILISKFGPKYGLVTYTTMSANLMLREIGIALF
                     LACVGLSAGEGFVETVVNGGYKWIAYGAIITVVPLLIVGTIGRKVYKINYFTLMGLIA
                     GSMTDPPALSYANATAGNDFPAVSYATVYPLTMFLRVITAQLMIL"
BASE COUNT         1251 a          867 c          956 g         1502 t
ORIGIN      
        1 ttttaacggc atgatgatac gatgctttta gtgcacccac cgatgtgcca agtatctctg
       61 acatatcttc gtatttcatc tcatcgaaat acttcatttg aaacactagc cgttgcttat
      121 ccggtaaagt taaaatagct tgttgaagca gcttttgtgc ttcatcccca tcaaaatact
      181 tatctccctc gagtgtgttt aacaggaagg aattctcatc atcgaccgat acgttgttct
      241 ggttcctttt tttattcaga aatgtgatgc tttcgttaat ggcaatccgg taaagccacg
      301 tcgttaattt tgattctcca cgaaaattat caatattgat ccaggcctta atgaaaacgt
      361 cttgtagaat atcgttggta tcttcgtgcg acaataccat ttttcgaatt tgccagtata
      421 atctttcgct gtattctgaa acaagtatgg cgaagccctg tcgttgggtt ctggggtcgc
      481 gtaacttttc taataactgt tcttcgctat atttgtccat aaaagggttg aagctgagtc
      541 acaaaggtaa cgtttttttt caatttttcc cgttgtcctc tccattttcg aaccagttga
      601 tgaaattttc agcatcggtt ttgttgtttt cccaccattc cagtgattcc ggaaagtaag
      661 aaacgcttat gtaacggcaa aaagtatcgt aatgattgga gttgaggatg cttttaaaaa
      721 aaggataatg gaaggtccag aaactcccgc tttttagagc tcccgattct ttttcgagtg
      781 cgctgaggat ggctttgttt acttctgtaa aaacaaaata gacatctgtg tccttttttg
      841 ctgcttttag ggagtccatc gaaaatttta tggcgttgta aataattttt cgattcaatc
      901 cttcttcggt tgagagagga ggcatttctt gctgaacact gttctcaggc atgtttcgta
      961 acaattgttg ctgaataaat gaccgctcta ccggtttttc cgtcaccggt ttcacaagca
     1021 ttgtctggag catctcttcg aaagcattct tggacctttt actgtcgggt tccaataata
     1081 aaaacaactg aaaggaataa atgcttctta cccaaagccc cttgtctctt tgtgcataag
     1141 cattcaaaag aaaggcaccg ctgttggttt tatccaactc gatggcacgg cttacgtgtt
     1201 caagggtctt atctatatcc ccttttttgt aataattcaa tgccatattg aaatgcaata
     1261 aataggaatc gccattcttt atcaaaccct cctgcagaat cgcaattgcg ttatcgattt
     1321 gtttcatctc ggccattgct tcacttttaa cagcataagc tccaacggaa aggtttttat
     1381 cgtttgaatt aataacacgt gtgctgtatt ttgaagcgtt ttcataatct ttcagttcaa
     1441 ggtacgaaag tgaaatttcg tataacgcca atgtcgattt tggatttatt ttcaatgcct
     1501 gttcaaactt ttcgatggcc ttcttgtatt ctgctgaatc gtgtagcgca actccttcat
     1561 gaataagggt ttgcacatta ttaccttgcc cgtttgtatt taaaattacg atggaaatta
     1621 aaaagagtat gatgagaatt ttcttcataa attacttttt gtggggtaaa tgtaagacaa
     1681 aaaaagagat atttaaattt ttcattaaaa aaagcggttt attctaacaa tcgtaaacaa
     1741 tttgtcttgt atttaaatat tttttagaag tcagtgtgtc gaataatgtt gtatgtttgt
     1801 atcctgaaat tcctgttaaa cgagcatgat agctgttttc aaacaagaac agggttaaag
     1861 gcgaaacgac attcgacaac cggatgggag tctactgcca cttgttggtg ttctcactgg
     1921 aagttggcga tctcgcttca ttcaattcag tacggcactt ttaaaataac tagttttaat
     1981 catatgaaat acgttgaggt aaattttatt tgtaatcccg attctgagat aattaccgat
     2041 gtgttggcag ctcaactctc cgatatcggt tttgagagtt ttgttaaatc aaatacgggg
     2101 ctgctggcct atgttcccga gccaacgttc tccacagaga agattgacac cttgctccag
     2161 aatttcttct tagatgcaga gattggctat tcgtttaaaa cgattgaaga tagaaactgg
     2221 aacgaggagt gggaaaagca ttattttcaa cccattgtat ttgaaaacaa atgcattatt
     2281 cacagttcat tccatcaacc cggcggggat tataattatc gtatccttat cgatcctaaa
     2341 atggcatttg gaacgggcca tcatcaaacc acagggttga tattgaaaga aattttatcg
     2401 atggacctgc aaaacaagtc tgttttggat atgggttgcg gaacagctgt tcttgccatt
     2461 ctggcttcaa tgtgtggtgc tgatcccatc attgcaattg atattgatga atgggcatac
     2521 caaaatgctt tggaaaacgt tcacttaaac cgtattaata atatcagcgt gttatgcggt
     2581 gattccggac tgttaggaaa cgatatgttt gatgtgattt tcgccaacat taaccggaat
     2641 attttgttgc aggacattcc ggcttacacg aaagttctaa atcctggtgg aaagcttgtc
     2701 atgagtggtt tttacaggga agatattccg gtgattcatg aaaaatgtgt gcagtatgga
     2761 ttaacgtatc gtgatttctc tgaaatggaa aactgggtgg ctgttgtatg tgaaaaatga
     2821 gctttcctgc gaattatctt ttgttttacc cccaaataag ttgtaacttt gcaccttatt
     2881 tcgtgtattc acattttttg tttaggttat tttttttatg gaatggatta atgatttgat
     2941 cttcggatcg ggtgttgcgc acgccatttt tgtacttgcg ctggtaattt cttccggaat
     3001 cctgctggga aaaattaaag tcttcggtgt ttctctcgga ataacgtgga ttctgtttgt
     3061 aggtatattt ttgggtcatc tgggtttagg gattgatgaa catattcttc attttataaa
     3121 agaatttggg ctcatccttt ttatctattc tattgggatg caagtaggtc ccagtttttt
     3181 ttcttccttc aagcaaggcg ggatcaccct gaatttattg gcctcatcgg tagtatttct
     3241 gggcgttatc accacttacg taattcatgt tataaccgga ttacccattt ctaccatggt
     3301 gggaatatta tctggagcgg taaccaatac cccgggactg ggtgcggcgc aacaaactta
     3361 ctttgacgta accggctccc aggatccttc tatagccatg ggatatgcag tagcctatcc
     3421 ccttggtgtt gtaggtatta ttcttagtct tatactcttt aagtcgctgt tcaggataaa
     3481 ttttgccagg gagaacgaag caatcagtat aacaaatgct tctaaaatga ccgaagcaca
     3541 aatgttttca cttcaggtac ataatccggc tgtttttggc aaaaccatcc acgagataaa
     3601 aagcctcatg gataaggaat ttgtcatttc cagagtcctg cacaacgatt ccggaatatt
     3661 aacggtgcct ctgccgcaca ccgtattgaa tgagaacgat aaaatactgg tggttacaaa
     3721 cctacaaaat attgaactta ttgaagcttt gatcggtcaa aaaatcgata tggatagaaa
     3781 cgagtggaat aagcttgaca atcaattgat ttcgcgaaga ataaatgtaa ccaaggcggg
     3841 aattaatgga aaatctatcg gacaattgcg gttaagaaat ttgtacggag taaatattac
     3901 ccgggttaac cgcgccggcg ttgatctggt tgctgatccg agactacagt tgcagctggg
     3961 tgaccgggta accgttgtgg gttccgaagc atccatcgct aatgttgaaa aattccttgg
     4021 aaactcgttg aaaaggctgc gtgaacccaa ccttatatct atttttatcg ggatcgccct
     4081 gggagtttta atcggaagca tcccttttat gtttcctgga atcccccaac ccgtaaaact
     4141 aggactggcg ggtggccctc ttattgttgc aattcttatc agtaaattcg gacctaaata
     4201 cggattggta acctacacaa cgatgagcgc aaacctgatg cttcgggaaa taggtatagc
     4261 gttattcctt gcatgcgtag gattaagcgc aggagaaggc tttgttgaaa ccgtggtaaa
     4321 tggcggatac aaatggatcg catacggagc cattatcacg gttgttccac ttttaattgt
     4381 gggtactatc ggaagaaaag tttataaaat taattacttt acgctgatgg gattgattgc
     4441 gggaagtatg accgatcccc cggctctttc atacgctaat gcgactgcag gaaacgattt
     4501 cccagcagta agctatgcga ctgtctatcc gcttaccatg ttcctgcggg ttattacggc
     4561 gcaattgatg attctg
//
LOCUS       DAOSDR010000263         4549 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrimonas sp. isolate AMR_MDS_1826
            NODE_6707_length_4549_cov_4.092790, whole genome shotgun sequence.
ACCESSION   DAOSDR010000263 DAOSDR010000000
VERSION     DAOSDR010000263.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863355
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrimonas sp. (wastewater metagenome)
  ORGANISM  Petrimonas sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Dysgonomonadaceae; Petrimonas.
REFERENCE   1  (bases 1 to 4549)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4549)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:10:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,720
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 35
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4549
                     /organism="Petrimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6707_length_4549_cov_4.092790"
                     /isolate="AMR_MDS_1826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2023866"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1324
                     /gene="atpD"
                     /locus_tag="PLI30_10065"
     CDS             <1..1324
                     /gene="atpD"
                     /locus_tag="PLI30_10065"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019246075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HOI79936.1"
                     /translation="MDTTDGLRRGTEVKALGQPISMPVGEQVKGRLMNVIGETIDELK
                     PLDHSGAYPIHRPSPKFEDLTTNQEILSTGIKVIDLLQPYLKGGKIGLFGGAGVGKTV
                     LIMELINNIAKGYSGYSIFAGVGERTREGNDLLREMIDSGVIRYGEEFKKSMDEGNWD
                     LSKVDYNELRKSQATLVFGQMNEPPGARASVALSGLSIAESFRDQSGEGNDILLFIDN
                     IFRFVQAGSEVSALLGRMPSAVGYQPTLATEMGLLQERITSTKQGSITSVQAVYVPAD
                     DLTDPAPATTFSYLDATTVLSRKISSLGIYPAVDPLESSSRILDPLIVGENHYNTAMR
                     VKQLLQRYKELQDIISILGMEELSDEDRITVNRARKVQRFLSQPFFMAAQYTGQPGVM
                     VPIDETIRGFTMILNGELDSYPEMAFLNVGTIDEAIEKGKKLMDQSQL"
     gene            1347..1601
                     /gene="atpC"
                     /locus_tag="PLI30_10070"
     CDS             1347..1601
                     /gene="atpC"
                     /locus_tag="PLI30_10070"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019540406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit epsilon"
                     /protein_id="HOI79937.1"
                     /translation="MQLEIISPEKMIFTGSVSSVTLPGASGSFQILENHASIISSLVK
                     GRLSFVTHQNELIELLVEDGFVEKNNNKVTVCLESIIKEL"
     gene            1598..2035
                     /locus_tag="PLI30_10075"
     CDS             1598..2035
                     /locus_tag="PLI30_10075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI79938.1"
                     /translation="MKRLVRHFLIVHTVMLFAAGFGVWYILKIFSPDTLIDAYFVLPL
                     FFYITGLVFIFIMMKMPKDNPKEIVNMYMLLRVIKVFIGVIIITLYWFLDKEQIRSFA
                     IIFIIFYLIYLGIETYIYMKIEMYLKEEQKKNNPVKKKYLKDR"
     gene            2032..3147
                     /gene="atpB"
                     /locus_tag="PLI30_10080"
     CDS             2032..3147
                     /gene="atpB"
                     /locus_tag="PLI30_10080"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009133047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HOI79939.1"
                     /translation="MKYPFHIWMWVCGLFLMALPLSASPESSPDPQAEELNVKEMILE
                     HLADTYEWKITGWDNGHITVPLPVILHSKTSGWHLFLSSKLHNDENSFNGFYVTHKGK
                     YKGKIVERDASGAEIRPFDLSLTKNAASLIFSSILLIILVMSVARSLRRNPMEGKKGF
                     IGLMEMFIMSIHDDVIKPAVGKDHARYAPFLLTVFFFIFLNNLLGLVPVFPGGANVTG
                     NIAVTMVLAISTLLVVNLTGTKEYYKEIFWPDVPKWLKVPVPLMPVIELVGVFTKPFA
                     LMIRLFANITGGHSTVMGLTAIVFVTASMGATINASMTALSVIFTVFIMLVELLVAFI
                     QAYVFTMLSAVFIGMARVESHQKEKELVETKYKTINK"
     gene            3163..3417
                     /gene="atpE"
                     /locus_tag="PLI30_10085"
     CDS             3163..3417
                     /gene="atpE"
                     /locus_tag="PLI30_10085"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019540402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HOI79940.1"
                     /translation="MELLSILLQATGSGLDVFGAALGVGIAVLAAGWGIGKIGTSAME
                     GIARQPEAAGDIRMNMIISAALIEGVALFAVVVCGFILIK"
     gene            3425..3925
                     /gene="atpF"
                     /locus_tag="PLI30_10090"
     CDS             3425..3925
                     /gene="atpF"
                     /locus_tag="PLI30_10090"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005937695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HOI79941.1"
                     /translation="MSLLTPEPGLVFWMSLSFGMVAFILMKYGFPVILQMVNKRKGYI
                     DNALLAAKQAHEELDKVKENSQTILEQTRLEQAKILEAAAQSRDRILEEAKERARIEA
                     DKIITDAQKQIEIEKESAVRAIRGEIASLSVSVAEKILREKLNSEEEQSKMINRLMDE
                     VIISKS"
     gene            3937..4491
                     /locus_tag="PLI30_10095"
     CDS             3937..4491
                     /locus_tag="PLI30_10095"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF009964.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HOI79942.1"
                     /translation="MNTGLISTRYATALLDFAVEQNLQERVYSEIKTLLHMYSEVSNF
                     REALANPAISIPEKRKLIHTASGESISKPLEQFIDLLLKNKREDRLQYIALRFLELYR
                     KKFNILSGTLITATKVDKPTYNRLKSFIESKTGETLELERLTDASILGGFILQIGDNR
                     LDTSVSGQLLRIKSELKEVNRKIV"
BASE COUNT         1350 a          969 c         1058 g         1172 t
ORIGIN      
        1 catggatacc acagacggat tacggcgcgg gacagaagtg aaagcattgg gacaaccgat
       61 cagcatgcct gtaggagagc aggtgaaagg gcgtctgatg aacgtgatag gtgaaactat
      121 agatgaattg aaaccgctcg accactccgg agcttacccc attcaccgtc cttctcccaa
      181 gttcgaagac ctgactacaa atcaggaaat tctctccaca ggaataaaag taattgacct
      241 gcttcaaccg tatctgaaag gcgggaagat aggacttttc ggcggagctg gtgttggcaa
      301 aaccgtgctg atcatggagt tgatcaacaa cattgccaaa ggttacagcg gatattccat
      361 ttttgccggg gtgggagaac gcacgcgcga agggaatgat cttttgcgcg aaatgatcga
      421 ttcgggggtg atccgttacg gcgaagagtt taaaaaaagc atggacgaag gaaattggga
      481 tttgagcaaa gtagattaca acgaactaag gaagtcacaa gccacattgg ttttcggaca
      541 gatgaacgaa cctcccggtg cccgggcatc ggtagcgctg tcgggactca gtattgccga
      601 atcgtttcgt gaccaatcgg gtgaaggcaa cgacatcctc cttttcatcg acaatatctt
      661 ccggtttgtg caggccggtt ctgaagtttc agccctactc ggacgaatgc cttcagcagt
      721 aggctatcaa cctacactgg caacagaaat gggcctgctg caagaacgta tcacatctac
      781 caaacagggc tctatcacgt cggtacaggc tgtatatgtt ccggcggatg accttaccga
      841 tccggctccg gcaaccactt tctcatacct ggatgccacc accgtgctga gccgtaagat
      901 ttcgtcgctt ggcatctatc cggctgttga tcctctggag tcatcttcgc gtatcctgga
      961 tccgcttatc gtgggagaga atcactacaa cacggccatg cgcgtaaaac aacttctgca
     1021 acgttacaag gaacttcagg acattatctc catcctgggg atggaagaac tttcggatga
     1081 agaccggatt acagtaaacc gtgcccggaa agtgcagcgc ttcctctccc aacctttctt
     1141 catggcagca caatacacgg gacaacccgg agtaatggtt cccattgatg agactatccg
     1201 gggatttact atgatcctca acggcgaact tgactcgtat cctgaaatgg ctttcctgaa
     1261 tgtgggcacc atcgatgaag ccatcgaaaa agggaaaaaa ttaatggatc aatcacagct
     1321 gtaagagtaa tttacattct tcgatcatgc aattagaaat tatctctccc gaaaaaatga
     1381 ttttcaccgg cagtgtcagc tccgtcacac tccccggcgc atcgggttca tttcagatac
     1441 tggaaaatca tgcatccatt atttcatcac tggtgaaagg aagactttcg tttgtaaccc
     1501 accagaacga actgatcgaa ttgcttgtcg aagatggttt tgtggaaaag aacaataata
     1561 aggtaacggt ttgtttggaa tcaattatca aagaattatg aagagattag tcaggcattt
     1621 tctcatcgtt cacacggtta tgttgtttgc agcagggttc ggagtatggt atatattaaa
     1681 aatattttcc cccgacacat taattgatgc gtactttgtg cttccgcttt tcttctacat
     1741 aaccggatta gtatttattt tcattatgat gaaaatgcct aaagacaatc ccaaggaaat
     1801 agtgaatatg tacatgctgt tgcgggtgat aaaagtattt attggagtga taatcatcac
     1861 tctttactgg ttcctggaca aagagcaaat acgcagtttc gccattattt ttattatttt
     1921 ttacctgatc tatctcggaa tagaaaccta tatctatatg aaaatagaaa tgtatctgaa
     1981 agaagaacaa aagaaaaata atccggttaa aaagaaatac ctgaaagatc gatgaagtac
     2041 ccgtttcaca tatggatgtg ggtgtgtgga ctgtttttaa tggctctgcc cctttcggcc
     2101 tcaccggaat ctagtccgga cccacaagcc gaagaattga atgtgaaaga aatgattctt
     2161 gaacatctgg cagacacata cgaatggaaa attaccggct gggataacgg acacatcacc
     2221 gttccgcttc cggtcattct tcacagtaag acaagcggat ggcacctgtt cctttcgtcg
     2281 aaattacata atgatgaaaa ttcatttaac ggattctacg ttacccacaa aggaaaatac
     2341 aaaggaaaaa ttgtggaaag agatgcttcc ggagccgaga taaggccgtt tgacctttcg
     2401 ctgaccaaga atgctgcatc gcttatcttt tcatccattt tgctgattat tctcgtaatg
     2461 agcgttgccc gttcactgag acgcaatccg atggaaggga aaaaaggatt tatcggtttg
     2521 atggagatgt ttattatgtc catccatgat gatgttataa aacctgccgt cggtaaagac
     2581 catgctcggt acgctccatt tctgctcacg gtgtttttct ttatttttct gaacaacctc
     2641 ctggggcttg tccctgtttt tccgggtggg gcaaacgtca ccggaaacat agctgtaacg
     2701 atggttttgg ccatttcaac cctgcttgtt gtcaatctta ccggaacaaa agaatattac
     2761 aaggaaattt tctggcccga tgtacctaaa tggctgaaag ttccggttcc gttgatgccc
     2821 gttatcgaac tggttggggt ttttaccaag cctttcgcgc taatgatccg tctttttgcc
     2881 aatattaccg gcggacactc caccgtgatg gggttgacgg ccattgtttt tgtaacggca
     2941 agcatgggtg caaccataaa cgcgtccatg acggcattat cggttatctt cactgtgttt
     3001 atcatgctgg tggagttgct ggtagccttt atacaggcat atgtgttcac aatgctgtct
     3061 gccgtcttta tcgggatggc acgcgtagaa tcgcatcaga aagaaaaaga acttgtggaa
     3121 acaaaataca agacaataaa caaataaact gattaaaaaa ctatggaact actttcaatt
     3181 ttattacaag ctacgggctc aggcctggat gtttttggtg cagccttagg tgttggcatc
     3241 gctgttctgg ccgccggatg gggaatcggg aaaatcggca catccgccat ggaagggatt
     3301 gccaggcaac cggaagcggc aggagatatc cgcatgaaca tgattatttc agctgccctt
     3361 atagagggag tagccctgtt tgccgtcgta gtctgtggat ttatcctgat aaaatagcac
     3421 cggtatgtcc ctattgaccc ccgaaccggg acttgtgttc tggatgtcgc tctcgtttgg
     3481 aatggttgca ttcattctga tgaaatatgg atttcccgta atcctacaga tggtgaacaa
     3541 acggaaaggg tatatcgaca acgcgttgtt ggcagctaaa caggcgcatg aagagctgga
     3601 caaggtgaaa gagaacagcc agacaattct ggaacaaacc cgcctggaac aagcaaaaat
     3661 actggaggct gcagcacaat cgcgtgaccg gatacttgaa gaagccaaag agcgtgcccg
     3721 tatagaagcc gataaaatca tcacagatgc tcaaaaacag attgaaattg agaaagaaag
     3781 cgcagtacgg gccatccgtg gcgagatagc atccctttca gtgagtgtgg ccgaaaagat
     3841 acttcgggag aaactgaact cagaggaaga acaatcgaaa atgataaacc ggctaatgga
     3901 cgaagtaatt atttcaaaat cgtaattctt tccttaatga acacaggact tatttccacc
     3961 cgatacgcta ctgctttgct ggattttgcc gtagaacaaa acctgcagga aagagtgtat
     4021 tcagaaatca aaacacttct acatatgtat tcggaggtga gcaacttccg ggaagccttg
     4081 gcaaatccag ccataagtat accggaaaaa agaaaactga ttcataccgc ctcaggggaa
     4141 agtatctcta aaccgttgga acagtttatc gatctgttgc tcaaaaataa aagggaagat
     4201 cggctgcaat atatagcctt gcgctttttg gaattatacc ggaaaaaatt caacatctta
     4261 agcggaacgc ttatcacggc cactaaggtt gataaaccca cgtacaaccg attaaagtcg
     4321 tttatagaaa gcaaaacggg tgaaacatta gaattggaac gactgacaga cgcatccatt
     4381 ttgggaggtt ttatacttca aataggcgat aatcgattgg atacaagcgt atcgggacaa
     4441 ctactccgga taaagagcga attgaaagaa gtgaatagaa aaatcgtctg ataataaaaa
     4501 acatgtctaa caatataata agaacgagcg aagtatcgga agtgttacg
//
LOCUS       DAOSDR010000264         4539 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrimonas sp. isolate AMR_MDS_1826
            NODE_6720_length_4539_cov_3.306869, whole genome shotgun sequence.
ACCESSION   DAOSDR010000264 DAOSDR010000000
VERSION     DAOSDR010000264.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863355
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrimonas sp. (wastewater metagenome)
  ORGANISM  Petrimonas sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Dysgonomonadaceae; Petrimonas.
REFERENCE   1  (bases 1 to 4539)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4539)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:10:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,720
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 35
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4539
                     /organism="Petrimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6720_length_4539_cov_3.306869"
                     /isolate="AMR_MDS_1826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2023866"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..949)
                     /locus_tag="PLI30_10100"
     CDS             complement(<1..949)
                     /locus_tag="PLI30_10100"
                     /inference="COORDINATES: protein motif:HMM:NF025092.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HOI79943.1"
                     /translation="MKKKCIALGDNHYCRIRKYLFAMKLTFLSFFVGIIGVSAASVYA
                     QEKKISLNLEKASIVEVFHEIEAQSEFVFIYKNEAIDLGNTVSVKINNTTVDKILDSV
                     LKNSGVKYEILDRQIIITPDRNQAVAPKQTNTVSPSGNSQQPQKKQLSGTVHDEKGSP
                     IPGATVMVKGTSVGIIADMNGNFSISVPMDSKVLLISFVGMKTKEVNIGNQSQFKIVL
                     QEENVGIEEVVVVGYGVQKKESVVGAITQVDNTALLRTGSTSVTNAITGKLSGVLTIQ
                     NTGKPGQNDAEIIVRGVSSWNNSAPLVLVDGVERDFKSLD"
     gene            complement(1084..2292)
                     /locus_tag="PLI30_10105"
     CDS             complement(1084..2292)
                     /locus_tag="PLI30_10105"
                     /inference="COORDINATES: protein motif:HMM:NF016648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR domain-containing protein"
                     /protein_id="HOI79944.1"
                     /translation="MREGNKITFISLLQDEEFLDLARNYSWSDIRIQEYIARFPEQQE
                     TIGYAFQFVKSTAPHNTLSPADEAGMRNFILDYAEKKKQPARLRRLTVNLSKVAAVLI
                     LALTGAYFLNSYFLRNRTLEKIAQNQVEVGEEAVLVLSDGSKHSLAAKESQIEYNADG
                     TEIVVRDHKREQEKLSNTQNVPGVAVNQIVVPYGHRHSIVLSDGTKVQLNSGSKLVFP
                     AEFKGSTREVFLRGEAFFEVSKKQGKTFIVKTDFLDLKVLGTSFNVSSYQDEKIVTTV
                     LVEGKVVVSQHNKLFRSDKIELNPGQGCFYSVANSSSEVREVDVSDFVLWREGVFKFK
                     DQPLKEVVSRVQKYYNKTIYVDGEKLQETIISGKLVLPEDFDEMMNYLARTLEARYEI
                     DNDQTYILKK"
     gene            complement(2392..2979)
                     /locus_tag="PLI30_10110"
     CDS             complement(2392..2979)
                     /locus_tag="PLI30_10110"
                     /inference="COORDINATES: protein motif:HMM:NF019265.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOI79945.1"
                     /translation="MTERTEFNLWIRFRNGDQDALAEIYDRYVRQLHSYGCKVHTDDA
                     LVKDCIQEVFIQLIDKRDRLTITETTHLYLFKSLRNKILEELRTQNRKKEISQSITDF
                     SPLAASVEQSIVFKEEELFRRELMEMALSSLTDYQREAIILRYSQEFSYEEIAEMLEI
                     DVPSARTLVYRSLKKVREFLSSRTQIFLFYLLHRF"
     gene            complement(3611..4465)
                     /locus_tag="PLI30_10115"
     CDS             complement(3611..4465)
                     /locus_tag="PLI30_10115"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOI79946.1"
                     /translation="MKRLLLLVIAAFFASVATYAQDAAALINNANEALKNKQYAQAFE
                     LYEKAMSNLGEVQVDSAINFNIGFAALQANKLDAAIKYFDKAIDAGTNVAKCYEFKAN
                     TYSKMKDFAKALENFEKAIEIAAADDVPGLAYNAAVTAYQGNNPEKAVLYFDKAIQGD
                     FKATDAIYYKAVVLNKMGKDAEYKQTLVEGAEKFPTEKKITAALANVYVSEGNEFYKK
                     GVEIINAANEKVTQGTLKTDDAAYTAEVDKSKVEFRKAVEVLGNAIKLDATNQNAVKL
                     MDACKKIL"
BASE COUNT         1295 a         1041 c          804 g         1399 t
ORIGIN      
        1 ggtccaaaga tttgaaatca cgctcaacac catcaaccag taccaacggg gcgctgttat
       61 tccaactgga aaccccgcga acaatgattt ctgcatcatt ctgtcccggt tttccagtat
      121 tttggatagt caaaaccccg gacaatttac cggttattgc attggtgaca ctggtactcc
      181 ctgtacgtaa aagggcggta ttatcaacct gggtaatggc tccaaccaca ctttcttttt
      241 tctggacacc ataaccaacg acaacgactt cctcaatccc aacattttct tcctgaagta
      301 ctattttaaa ctgggattgg ttgccaatgt tgacctcttt cgttttcatc cccacgaagc
      361 tgattaaaag cacctttgaa tccatcggca cacttatgga gaaattacca ttcatatcgg
      421 caataatccc aacgctggtt cctttaacca taactgttgc tccgggtatt ggcgatcctt
      481 tttcgtcgtg caccgtacca cttagctgtt tcttctgtgg ttgctgtgaa tttccgctcg
      541 gtgaaacagt attggtttgt ttgggcgcta ccgcttgatt tcggtcagga gttatgatga
      601 tctgtctgtc cagaatttca tatttcacac ccgaattttt caagactgaa tcaagaatct
      661 tatcaacagt agtattgttg atttttacac tgaccgtatt tcccaggtca atcgcttcgt
      721 ttttatatat aaatacaaac tcactctgtg cttctatctc atgaaatacc tcaacaatcg
      781 atgctttctc aagattgagt gatatcttct tttcctgagc ataaaccgac gctgccgata
      841 ctcctattat cccaacaaag aaggataaga atgttagttt catagcaaat agatatttcc
      901 ttatgcgaca atagtgattg tcacctaagg caatacattt ttttttcata aattcgtaat
      961 gttaaaataa ttctacattt taataactta aaacatcgac cggtggatgg ttccagcatt
     1021 caccggtctt tttggtttag ttgaatcagg tttcactata tcataggcaa aagactttat
     1081 aaattatttt ttcaagatgt atgtttggtc attatctatc tcatatcggg cttcgagggt
     1141 acgggccagg tagttcatca tctcatcaaa atcttccgga agcaccagct ttccggaaat
     1201 aatcgtctcc tgaagcttct ccccgtcaac ataaattgtt ttgttataat atttctgaac
     1261 ccgtgaaaca acttccttta gaggctgatc cttaaacttg aaaactcctt ctctccacaa
     1321 aacaaaatca ctaacatcga cctctctaac ttccgaactg gaattggcca ccgaataaaa
     1381 gcatccctgc ccaggattca gttcaatttt atcgctcctg aacaatttgt tatgctggga
     1441 aacgacaact tttccttcga ccagcacagt tgttacaatt ttctcatcct ggtaggaaga
     1501 gacattaaat gaagtcccca aaactttcag atcaagaaaa tctgttttta caatgaaagt
     1561 ttttccctgc tttttggata cttcaaaaaa ggcctcgcct cttaaaaaaa cttcacgggt
     1621 acttccttta aattcagccg gaaataccag cttactgcct gaattcagct gaacctttgt
     1681 cccgtcgcta agtacaatgc tatgacgatg tccgtaaggc accacgatct ggttgactgc
     1741 cacaccggga acattctgcg tattggaaag cttttcctgt tctctcttgt gatcgcgaac
     1801 cacaatctca gttccgtcag cattgtattc gatctgcgat tcttttgctg ccaaactgtg
     1861 ttttgatcca tcggacaaaa caagaacggc ctcctctccc acctcaacct ggttttgcgc
     1921 aattttctcc agcgtgcgat ttctcagaaa ataggaattc aaaaaatagg cccccgttaa
     1981 tgccaaaatc aaaactgctg ctactttcga caggttaacc gtcaaccggc gtaaacgggc
     2041 aggctgcttt ttcttttcgg cataatccaa aatgaaatta cgcatccctg cttcatcggc
     2101 aggagacagc gtgttgtgcg gtgcggtgga ttttacaaat tggaaagcgt aaccaatcgt
     2161 ttcttgttgt tcgggaaacc gggcgatata ttcttgtatc cgaatatcgg accaactgta
     2221 atttctggct aagtccagaa actcttcgtc ttgaagcaga gaaatgaatg ttatcttgtt
     2281 gccttccctc atttagacaa taaatgatcg aaaagacgat ttatagacaa gatttactca
     2341 ctttttccgg cttaatttca aaatttataa aaccccctaa atatcaggaa attaaaatcg
     2401 atgtaacaaa tagaaaagaa agatttgggt gcgggaagaa aggaattccc tgaccttttt
     2461 caaagaccgg tacaccaatg tcctggccga aggaacatca atttcaagca tctctgctat
     2521 ctcttcataa ctgaactctt gagaatacct aagaataatt gcttcacgct gataatcagt
     2581 caaagaactc agggccattt ccatcaattc ccgtcgaaaa agttcttcct ctttaaaaac
     2641 aatcgattgc tcaacactag ccgccaaagg ggaaaaatca gttatcgatt gagaaatttc
     2701 ttttttacgg ttttgcgttc gcaactcttc caggatttta tttcgcaacg atttaaacag
     2761 gtataaatgg gtggtttcgg taatggttag ccggtcccgc ttatcgatta gttgaatgaa
     2821 tacctcctga atacaatctt taacaagcgc atcatcagta tgtactttac agccatacga
     2881 atgtaattgc cgaacatatc gatcatatat ttccgccaag gcatcttggt ccccgtttct
     2941 aaaccggatc cacagattaa attccgtacg ttctgtcact gacaatctct cttgattttc
     3001 cctgaattcc aaaatctcta ttctacggag aattttgaat cactccagta tcttttaata
     3061 cacaaaagcc gcaaaacggc ttctcataag tacataaaaa tgttgaattt tcactaacat
     3121 tctgaaaaaa caacacagaa ataatttcgt ggcaaacagc tgcaaaacca acacattaat
     3181 attttgctac aaatttaaaa ttcatgtgat acatgatcag gtaaataagg cagttttgca
     3241 aaaaaaaatg aatttcaacc ggcaatgaac cccgtatctg aaggcaggca gccatcaatc
     3301 attctattct cctcagagaa gtctggcttc tacattgcga tcagacagac aacttcgtaa
     3361 aaaaccagtt ttttacaaag ggcacaggtt agctaaacaa agaacttaca cgtgtccgat
     3421 tcaatccgga ttgtttatga gaaggggaaa cgaattcact actttttgat acaactctgg
     3481 gtgccaatat ttttaccaac cgacacaaag tcgccttgta aaacactgac acccagaact
     3541 atatcttgag aatatttttc taactgacaa tcagttatct gatattttta tcaagcacta
     3601 aataaaagaa ttataaaatc ttcttacagg catccatcaa tttaacggca ttctggttgg
     3661 ttgcgtccag ttttattgca tttcccagaa cttcaacggc cttgcggaat tcaactttcg
     3721 atttatccac ttcggcggtg taagctgcat cgtcggtttt cagagttccc tgggttactt
     3781 tttcattggc ggcattgatg atttcaactc cttttttgta gaactcattt ccttctgaaa
     3841 cataaacatt ggccagcgcc gcagtgatct tcttctcggt cgggaatttt tcggctccct
     3901 ccaccagcgt ctgtttgtac tcggcatcct tgcccatttt attcagtaca acagctttgt
     3961 agtagatggc atcagtggct ttgaaatctc cctgaattgc tttgtcgaaa tagagcacag
     4021 ccttttcggg gttgttgccc tggtaagcgg taacggcagc attgtaagcc agtcccggca
     4081 catcatcggc agcggcaatt tcaatagctt tctcgaaatt ctcgagcgct ttggcaaaat
     4141 ccttcatttt ggagtaggta ttggccttaa actcataaca ttttgccaca ttggtaccgg
     4201 cgtcaattgc cttgtcgaaa tacttgatgg cagcatccag tttgttggcc tgcaaggcag
     4261 caaaaccgat attaaaattt atggcgctgt caacctgaac ttcgcccaga ttggacatcg
     4321 ctttttcata caattcaaaa gcctgggcat actgcttgtt tttcaacgct tcgttggcgt
     4381 tgttgattaa agcggcggca tcctgggcgt atgtcgctac cgaggcaaaa aacgcagcaa
     4441 ttactaacag caacaatctt ttcatcttcc ttaaaatttt aacgattcgt aatttatact
     4501 attgatttaa ttatactaaa tagtcaacgc gacttccaa
//
LOCUS       DAOSDR010000265         4486 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrimonas sp. isolate AMR_MDS_1826
            NODE_6830_length_4486_cov_2.176710, whole genome shotgun sequence.
ACCESSION   DAOSDR010000265 DAOSDR010000000
VERSION     DAOSDR010000265.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863355
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrimonas sp. (wastewater metagenome)
  ORGANISM  Petrimonas sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Dysgonomonadaceae; Petrimonas.
REFERENCE   1  (bases 1 to 4486)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4486)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:10:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,720
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 35
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4486
                     /organism="Petrimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6830_length_4486_cov_2.176710"
                     /isolate="AMR_MDS_1826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2023866"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..913)
                     /locus_tag="PLI30_10120"
     CDS             complement(<1..913)
                     /locus_tag="PLI30_10120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019539101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HOI79947.1"
                     /translation="MKIYETAVKNPVGTSLVSIGLVLIGLMFYQQLPVDLFPEIELNM
                     VSVMTSYPGAGPDDIESNVTRPLENVLNSTENLEEISSVSKDNVSVIMLEFEWGSDMN
                     AIMNDVRDKVDLVSGALPDGTNDPMIIKFSSDMAPVMILSATARESSDALYKILDEQI
                     VAPLNRIQGVGTVALSGAPQREVQVNVIPAKLEAYNISLEQIARVIAAENLNVPAGDL
                     DIGSQTYMLRLEGEIEESRELNNIVVGSSLGQAIYLKDVATVNDTVQSRIMESYTNGE
                     RGATIMIQKQTGANSVAIVDAIAKQLPE"
     gene            complement(1012..2034)
                     /locus_tag="PLI30_10125"
     CDS             complement(1012..2034)
                     /locus_tag="PLI30_10125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019539116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HOI79948.1"
                     /translation="MKQNNVLKFTLLAALLLLASCGGGSTKNETQSETVKQNVKIEEV
                     RLTPVDQLSTFTATVEANTVNNIAPAIGGRRIRSIHVDVGNLVGKGQTLVTMDAANYS
                     QQETQLATLKRDYERYRELYEVGGVSKQQLDQLKAQLDVAQSALNTLDENTRLTSPIS
                     GVVTARNYDPGDVTAAMPILTIENINPVKVIVHVSESFYNQVVKGMPVHVQVDALANE
                     VFEGKVSLIHPTINPGSHTFPVEIEVNNNDLRLRPGMFSRVTINFGSYDRPLVPDMAV
                     LKQTGSNDRYVFVEKDGIAIYTLIQLGSRIEDKYEIISGLSAGDRVIVQGHTGLIDGA
                     EVQVVD"
     gene            complement(2056..3504)
                     /locus_tag="PLI30_10130"
     CDS             complement(2056..3504)
                     /locus_tag="PLI30_10130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019539115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HOI79949.1"
                     /translation="MKAKLLRIFLMMACGLFGLSTTTAQNSNSLIVDLKTAIDIALSE
                     NPSVKVADMEIEKKTYAQKSAYGALLPQIDLIGQFQHTLKRQTMYLDTDMGSLFGPGS
                     IDPGNFTPEELQVAEVLGKLMTPDPNAADEGIQVGRKNMWTGTLNVSLPLVVPSLWKN
                     IQMSKVDLEIAVEQSRSSKIDMTHQVKKAFYSILLAKDSYNLFRETYTTDSLNLVNIK
                     NKFEQGIVAEYDVITADVRLKSIIPNILQSESMLKIAELQLKMLMGIDAEIPVEIVGS
                     LADYEVWMFEAIVPSDNNLLENSELRQFDLQAMQARNAHEMQKLQRLPTLTSSFNYSY
                     ISQNNNFKFNTYRWDPYSMVGVTLAIPIFSGGQRYHNIKQSEIQLLQLQEQRHNLKRG
                     LQLSVKNSVEFINKSIEQVVAAESSVQQAKKGYEITQKRYDTGMGTIVDLNAAALAVT
                     NAELQYRNAIYDYLASKADLEKTLGYDINPTN"
     gene            complement(3646..4263)
                     /locus_tag="PLI30_10135"
     CDS             complement(3646..4263)
                     /locus_tag="PLI30_10135"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HOI79950.1"
                     /translation="MTLKERIVIKAGTLFYQYGIKNVSMDDLASSLGISKRTLYENFR
                     DKEEVLLTYMAGKRDERNNKIVEAINRSENMIEVFLYLIEYHKNKDLPSIKFYEDIYK
                     YYPRIYKLIQQDVENNKIHIKTYLQQGIDQGYIRGDLNVDVAAFLVEETTYTYIRAAY
                     LEKPPFSFQQLFFTMMVNFIRGISTYKGIQIIDDYLTNNNSSVFF"
BASE COUNT         1201 a          977 c          880 g         1428 t
ORIGIN      
        1 gttccggaag ttgttttgca atcgcatcaa caatagctac cgaattggct cctgtttgtt
       61 tttgtatcat gatggtggca ccgcgctctc cattggtata actttccatt atgcgagact
      121 gcaccgtgtc gttcacggtg gccacatctt tcagataaat ggcttgtcct aaactgctgc
      181 caaccactat gttattgagc tcgcgacttt cttcaatttc accttcgaga cgcaacatat
      241 aggtttgtga accaatatct aaatctccgg caggaacatt caggttctca gcggcaataa
      301 cccgcgctat ttgttccagg gaaatgttat acgcttccaa ttttgcaggt atcacattca
      361 cttgaacttc acgctgcggt gcacctgaaa gtgcaacagt tcccacaccc tgtatacggt
      421 tgagcggggc gactatctgt tcatcaagaa ttttatacaa cgcgtccgaa ctttcccggg
      481 cagtggccga aagaatcatt acaggtgcca tatcggaact gaacttaata atcatcggat
      541 cattggtgcc atcgggaagc gctcccgaaa ctaaatctac cttgtcgcgt acatcgttca
      601 tgatggcatt catatcagac ccccattcaa actcgagcat aattaccgag acattatctt
      661 tcgataccga tgaaatttct tcgagattct ctgtagagtt aagtacattt tccaacggcc
      721 ttgtgacatt cgattcaatg tcatcagggc cggcaccggg ataagaagtc atcaccgaca
      781 ccatatttag ttctatttcg ggaaacaaat cgacaggaag ctgttgataa aacataagtc
      841 cgataagaac aagaccgata gataccaacg atgttcccac aggatttttt accgcggttt
      901 catatatttt catttgttac tattttttct attgttaaaa ttgattgccg ctaaattaaa
      961 attaaaatta aaattcggaa atgtaaattt taaaattcat ggtttaagct gttaatcaac
     1021 cacctgcact tcggctccgt caattaaacc ggtatgtccc tgcacgataa ccctgtcgcc
     1081 tgcacttaaa ccggaaataa tctcgtattt atcctcgatt cggcttccca gttgaataag
     1141 cgtgtagatg gcgataccgt ctttttctac aaaaacgtat ctatcattgg agccggtttg
     1201 ttttaatact gccatgtcgg gtacgagtgg acgatcgtaa ctcccgaaat ttatggtcac
     1261 acgtgaaaac attcccgggc gaagacgcag atcgttgtta tttacttcta tttcaaccgg
     1321 gaaagtatgt gaacccggat ttatcgtggg atgaatcaat gacactttac cttcgaaaac
     1381 ttcgttggca agcgcatcaa cctgaacatg gacgggcatt cctttaacca cctggttgta
     1441 aaacgattcg gaaacatgta cgattacttt caccggattg atattctcaa tggtaagaat
     1501 aggcatcgct gctgttacgt ctcccggatc ataatttcgt gcggttacca ctccgcttat
     1561 cgggcttgtc aaacgggtat tttcatccag cgtattaaga gcagattgtg ccacgtcgag
     1621 ctgcgctttc aactggtcga gttgttgttt ggaaacgcca cctacttcat atagttctct
     1681 atatcgctcg taatcgcgtt tcagcgtagc caattgcgtt tcttgctgcg aataattggc
     1741 agcatccatc gtaacaagtg tttgtccttt gccaacaagg ttacctacat ccacatgaat
     1801 gcttcgtatg cgtcgtccgc ctatggctgg tgctatatta ttcactgtgt ttgcctcaac
     1861 cgttgcggta aaagtcgata gttgatcgac aggggtcaat cggacttctt ctatctttac
     1921 gttttgctta actgtttcag attgtgtttc attttttgtc gatcctcctc cacaagatgc
     1981 gagtaaaaga agagctgcca gcagcgtaaa tttcagaaca ttattctgtt tcataaatca
     2041 agttatttat attttttagt ttgtgggatt aatatcgtaa ccgagtgttt tttctaaatc
     2101 ggctttggat gccagatagt catagattgc attacggtat tgcagctcgg cattggtcac
     2161 tgcgagtgca gccgcgttta aatctacaat agttcccatt ccggtgtcgt atcgtttttg
     2221 tgtgatctcg taacctttct ttgcttgttg taccgacgac tcggcggcga caacctgttc
     2281 gatgctttta ttgataaact ctacactgtt tttcacagat agttgcagac cgcgtttcag
     2341 gttatgacgt tgctcttgca gttgtaataa ttgaatttcg gactgtttaa tgttgtgata
     2401 ccgttgtcct ccgctaaaaa tagggatggc aagcgtcaca cctaccattg aataaggatc
     2461 ccagcgatag gtattgaact tgaaattatt gttctggctt atataggaat agttgaatga
     2521 tgaggtaagc gtaggtaaac gttgaagttt ttgcatctca tgtgcatttc tcgcctgcat
     2581 ggcttgcagg tcgaattgtc tcaattcact attttcaagc aagttattat cagagggaac
     2641 aatggcctca aacatccata cttcataatc agcaagcgaa cctacgatct ccacgggtat
     2701 ctccgcatcg atgcccatca gcatttttaa ttgtagttcc gcaattttca acatgctctc
     2761 tgactgcaga atattgggaa taatgctttt gaggcggaca tcggcggtaa tgacatcgta
     2821 ttcggcaacg ataccctgct caaacttatt ttttatattc accaaattca aagagtcggt
     2881 cgtatatgtc tccctgaaca gattatagct gtcttttgcc aataatatac tgtaaaaggc
     2941 cttttttacc tgatgtgtca tatcaatctt cgatgagcgc gactgttcaa cggctatctc
     3001 caggtcgacc ttactcatct ggatgttttt ccataacgag gggaccacga gcggtaaact
     3061 aacattcaat gttcccgtcc acatgttttt tctgcctacc tgaatgcctt catcagcggc
     3121 attgggatcg ggcgtcatca acttgcccag tacttccgcc acctgtagtt cttcgggagt
     3181 aaaatttccc ggatcaatgg aaccgggtcc aaaaaggctg cccatatccg tatccaaata
     3241 catcgtctga cgtttcaagg tatgctggaa ctgtccgata agatcaattt ggggcaacag
     3301 ggcgccatag gcgctttttt gagcatacgt ttttttctca atctccatat cggcaacttt
     3361 taccgatgga ttttcactta atgcaatatc gatagcggtt tttaaatcaa cgatcaaact
     3421 atttgaattt tgtgccgttg ttgtggataa tccgaacaat ccacaagcca tcattaaaaa
     3481 gattcttaat agtttagctt tcataaaaag taattaattg ttttcgtatg ttttgtttta
     3541 atagttcggt aaatttttat ttaatgcgca cataatcagc aaattatccc cttcatttta
     3601 aaaggaagag tagacactga taatcaatca gttatgggcg attctttaaa aaaataccga
     3661 actgttgttg tttgtaagat aatcatcaat aatttgaatt cctttatagg tagaaattcc
     3721 gcgaataaaa ttcaccatca tcgtaaaaaa aagctgctga aaggaaaaag gcggtttctc
     3781 aagatacgcc gcacgaatat acgtgtatgt ggtttcctct accaggaagg ctgccacgtc
     3841 cacgttcagg tccccgcgga tataaccttg atcgattccc tgctgcaggt atgtttttat
     3901 atgtatttta ttgttttcaa catcttgctg aatcagcttg tagatccggg gataatactt
     3961 atagatatct tcgtaaaatt taatggaagg caggtcctta tttttatggt attcaatcaa
     4021 ataaagaaat acctcaatca tattttccga ccggtttatt gcctccacaa tcttattgtt
     4081 cctttcatcg cgcttgccgg ccatgtaagt caataagacc tcctccttat cccggaaatt
     4141 ctcgtaaaga gtgcgttttg aaatgcctaa agaagacgcc aagtcgtcca tagagacgtt
     4201 tttaataccg tactggtaaa aaagagtacc tgcttttatt actattcttt cttttagtgt
     4261 catcgtattt aaaaagacgc cacaaaggta atccgaaaaa ctcaaaacat caaatcggtc
     4321 ttccaggttt taacaaactt tacaataaga tcttgttaca agcccagctt ttcccgaagc
     4381 cgtttatatc cggaagcact gatttttatt ttatcgccgt tgctcagggt gagcagttga
     4441 ttttgtttct cgtacaattc gatgcgcaat atttttaccg tgttga
//
LOCUS       DAOSDR010000266         4455 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrimonas sp. isolate AMR_MDS_1826
            NODE_6886_length_4455_cov_3.910909, whole genome shotgun sequence.
ACCESSION   DAOSDR010000266 DAOSDR010000000
VERSION     DAOSDR010000266.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863355
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrimonas sp. (wastewater metagenome)
  ORGANISM  Petrimonas sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Dysgonomonadaceae; Petrimonas.
REFERENCE   1  (bases 1 to 4455)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4455)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:10:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,720
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 35
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4455
                     /organism="Petrimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6886_length_4455_cov_3.910909"
                     /isolate="AMR_MDS_1826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2023866"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2361
                     /locus_tag="PLI30_10140"
     CDS             <1..2361
                     /locus_tag="PLI30_10140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019540674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HOI79951.1"
                     /translation="TESAILEDLLKKMPGIEIDSEGKITVNGREIKKIMVDGEEFFST
                     DPKVASKNLPAKMVEKLQVLDRRTDMAQMTGFDDGEEETIINLMVKPGMKEGLFGNAF
                     VGYGTKDRYEGNAMVNYMKDKNQYTVLGGFNNTNNAGFSDLASSMFGSGGGGGRRMFF
                     GGRSGITTSGNAGFNFSQQFTNKLKLGGNLRYGNTDNNTLSKTHTQNILSSGNTLEGE
                     NNSSNNYSQNFNMDLRLEWTPDTLTRIIFNPEGSVYNNRRTELSDFLTTTETLEDSIN
                     YGDSRYNSTGDGKNLSARLDVSRTLGKKGRILSVQLRGGMSDSENEGTNLSNTFYNGT
                     KPDDLIDQRFVNTNDSKNWRGYVSYVEPLGNNNAIQFAYQYRQNISGSDKDTRVKDES
                     GNYTVLDEQYSKRLENNFTNQEIEFNFQSVRQKYDYTIGFSVQPSSSQSKTFIGDNKI
                     SDFTRDVVNYAPMAQFNYRWTRQHNLRLRYFGNTDQPSVTQLSPVVDVSNPLNITYGN
                     PDLKPSFEHRLNLRYQNFNPEKNRSMGFFGDIRYLTNDIVSSTMTDRETGRRETTYEN
                     VTGNWNANGRMMMNIPLKNIRFSVFSMSFASYNHANGFSNLEKNLSRRLNLGETLGLN
                     YRSDLIDFGIRGNISYNKVKNSLEGQRDQEFLNYGGNANTTLYLPWDMSIESDINYST
                     NSGYSDGFTQDEWLWNASIQKQLFKQKNGTIRLKIYDILQQRSNISRSVTSNYIRDTT
                     TNTLTTYFMVHFVYRFNIFKNGATREDMMPQHGPGPGRGFGRGHGG"
     gene            2409..3482
                     /locus_tag="PLI30_10145"
     CDS             2409..3482
                     /locus_tag="PLI30_10145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005786905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HOI79952.1"
                     /translation="MENRIRNNTTNTYAVILVHILLWSMIVVAPYFFIDRERLFRWDL
                     FIRSTPDTLGLLLVFYINYFLLIKQFLFKGRTREFIFYNLLLIVAAAVLMHFGNDLLR
                     WISPEHMPRRGGRKFRPSPWLFVIRNLTTLVTMMGLSVALKMTFRWFEVESERKELEK
                     AKSEAELQNMKNQISPHFLLNTLNNIYALVEFNPSKAQQAVLDLSKLLRHILYDNDKP
                     LVPLHQEVDFIKNYVDLMRIRLSENVKLNTQLSVKTNSGTPIAPLIFISLIENAFKHG
                     ISGNKPSFIDISLSETPDGKIEFVSRNSYYPKSEADKSGSGIGLGLVKKRLEMAYPGR
                     YQWDSEVTGDTYSTTLIINTKED"
     gene            3490..4200
                     /locus_tag="PLI30_10150"
     CDS             3490..4200
                     /locus_tag="PLI30_10150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009120412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HOI79953.1"
                     /translation="MKLNCWIVDDEPLALELLDSYVQKTPFLNSTGKYSSAIQAMNNM
                     PNERIDVIFLDIQMPEVNGMEFARFLDKNTRIIFTTAFSEYALDGYKVNALDYLLKPF
                     SYSEFLSASKKALEWFEMKAASEEKGESEVSGIFVRSDYKLVHVLFDDILLVEGLKDY
                     IKIFTGKDPKPILTLMSMKSMEDELPATRFIRVHRSFIVNRDKIVRVEKNRIIIGKHE
                     IPIGETYRKAFMEEINGK"
     gene            complement(4213..>4455)
                     /locus_tag="PLI30_10155"
     CDS             complement(4213..>4455)
                     /locus_tag="PLI30_10155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005864731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macro domain-containing protein"
                     /protein_id="HOI79954.1"
                     /translation="RNEESLLKECYVNSLKLADEQGLKSIAFPNISTGVYRFPKERAA
                     EIAVDAVKNFPAKFVEEVTFVCFDEENFEIYKRRLE"
BASE COUNT         1354 a          936 c          977 g         1188 t
ORIGIN      
        1 accgaaagtg ctattctcga ggatctgctg aaaaagatgc cggggattga aatagattcg
       61 gaagggaaga taaccgttaa cggccgtgaa ataaaaaaaa tcatggtaga cggtgaagag
      121 tttttcagca ctgacccgaa agtggcctcg aagaatctgc ccgccaaaat ggtcgaaaag
      181 ttgcaagtcc ttgaccggag aacagatatg gcgcagatga ccggatttga tgacggagaa
      241 gaagagacca tcatcaacct gatggtaaaa cccgggatga aagaaggttt gtttggtaac
      301 gcctttgtgg gatacggcac aaaagaccgg tacgaaggga atgcgatggt caattatatg
      361 aaggacaaga accaatatac tgttttgggt ggttttaaca acaccaacaa tgccggattt
      421 tccgatttgg catcttcgat gtttggaagt ggaggtggtg gaggtagaag gatgttcttt
      481 ggcggacgaa gcggaatcac cacatccgga aatgcaggtt ttaatttcag ccagcaattt
      541 accaataaac tgaagctcgg cggtaatctc cggtatggaa atactgacaa caatacactt
      601 tccaaaacac atacacaaaa tattctcagc agtggtaaca cattggaagg cgaaaataat
      661 tccagcaaca attacagcca gaatttcaac atggacctgc gcctggaatg gacacccgat
      721 acgttgacaa gaattatttt taatcctgaa ggatcggttt acaacaatcg aaggactgag
      781 ctcagcgact ttctgaccac aaccgaaacc ctggaggatt ccataaatta cggagattca
      841 agatataatt ccacgggtga tgggaagaac ctttctgcgc gtttggatgt gagccgtaca
      901 ctcggtaaaa aaggccgtat attgagtgtc cagttgcgtg gaggaatgtc cgattctgaa
      961 aatgaaggga caaatctatc caacactttt tacaacggga cgaaacccga tgaccttatt
     1021 gatcagcggt ttgtcaatac caatgattcc aaaaactggc gcggttatgt atcttacgtt
     1081 gagccgttgg gaaacaacaa cgctattcag tttgcctatc agtatcgcca aaatatttcc
     1141 ggatcggata aagatacccg tgttaaggat gagtcaggta attacacggt tttagatgaa
     1201 cagtacagca aacgtctgga aaataatttc accaatcagg agattgagtt taactttcag
     1261 tctgttcgtc agaagtacga ttatacgatt gggttctctg tacaaccttc aagctcacaa
     1321 agcaaaacgt ttataggaga caataaaatc agtgatttta cccgagatgt ggtcaattat
     1381 gcacccatgg cgcagttcaa ctacagatgg acacgacagc ataacctgag gctccgttat
     1441 tttggcaata ccgatcaacc ttcggtaacc caactgtctc cggtggtgga tgtttcgaac
     1501 ccgctgaaca tcacttatgg gaatcccgat ttgaaacctt cttttgaaca ccggctaaac
     1561 cttcgttacc agaattttaa tcccgagaag aaccgttcca tgggtttttt tggagacatc
     1621 cgttacctga ctaatgatat tgtctcatcc accatgaccg accgggaaac gggacgcagg
     1681 gaaaccacgt atgaaaacgt gacagggaac tggaatgcca acggccgtat gatgatgaat
     1741 atcccgttga agaacatcag attctctgtt ttctccatgt catttgccag ctataatcat
     1801 gcaaacggtt tctctaatct ggagaaaaac ctgagccgca ggctcaatct gggtgaaacg
     1861 ctcgggttaa actaccggtc ggacctgata gactttggga tccgcggtaa tattagctat
     1921 aataaagtaa aaaacagcct ggaaggacaa cgcgaccagg agttcctgaa ctacggggga
     1981 aatgccaata ccacccttta ccttccctgg gatatgtcca ttgagagcga tatcaactac
     2041 agtaccaatt caggttattc cgatggtttt acgcaagacg agtggttatg gaatgcttcc
     2101 attcagaagc aattgtttaa acaaaagaac ggcaccatcc gtttaaagat atatgatatt
     2161 ctgcaacaac gtagcaacat cagtagatct gtgacgtcca actatattcg tgacacaaca
     2221 accaacacgc ttaccaccta cttcatggtg cactttgttt atcgtttcaa tatttttaaa
     2281 aatggagcaa cacgagaaga catgatgcct cagcacggtc ccggtcccgg aagaggtttt
     2341 ggacgtggcc atggcggtta atgctacgga gacaccttta attacccaaa ttttaaactg
     2401 gatttacaat ggaaaatcgc atcaggaaca acacgaccaa tacatacgca gtcattcttg
     2461 tgcacatttt attgtggagc atgattgtgg ttgcacccta tttttttatt gaccgggaaa
     2521 ggctttttcg ctgggacctg ttcatccgtt caacacccga tacattgggg ttgttgctgg
     2581 ttttctatat caattatttt ctgctgataa aacagttttt atttaaaggg agaacaaggg
     2641 aattcatttt ctataacttg ctgttgatcg ttgccgctgc cgtactcatg cattttggaa
     2701 atgatttgtt gcgatggata agcccggaac atatgccgcg acgcggtggc cgtaaattca
     2761 ggccttcgcc ctggcttttt gtcatccgga acttaactac tttagtaacc atgatgggat
     2821 taagcgttgc cctgaaaatg acttttcgtt ggtttgaagt ggagagtgag cggaaagagc
     2881 tggaaaaagc aaaatcggaa gctgaattac aaaacatgaa aaatcagatc agccctcact
     2941 ttctgctcaa caccctcaat aacatctatg cgctggtgga atttaatcct tccaaagcac
     3001 aacaggctgt gctagatttg agtaaactat tgcgtcacat cctttacgac aacgacaaac
     3061 cgcttgttcc tttgcatcag gaagtggatt ttatcaaaaa ttacgttgac ctgatgcgga
     3121 tcaggctgtc tgaaaatgta aagctgaaca cacaactttc agtaaaaaca aacagcggta
     3181 cacccatagc accgttaatt tttatatctt tgattgaaaa tgcttttaaa cacgggatca
     3241 gcggcaataa accatcattt atcgatattt ctttgtcgga aacacccgat ggaaaaatcg
     3301 aatttgtttc ccggaacagt tactatccca agtcggaggc agacaagagc ggtagcggaa
     3361 tcggactagg gctcgttaaa aaacggttgg aaatggctta tccagggcgg tatcaatggg
     3421 attccgaagt taccggagac acctactcca ccactttgat catcaatacg aaagaagatt
     3481 aataacgtta tgaaactgaa ttgctggata gttgacgatg agccactggc attggaattg
     3541 cttgattctt atgttcagaa aacacctttt ttaaactcga ccggcaaata ctccagcgcc
     3601 atacaggcaa tgaacaatat gcccaacgag cgtatagatg tgatttttct cgacattcaa
     3661 atgcctgaag tgaacggcat ggaatttgcg cggttcctgg ataaaaacac tcgtattatt
     3721 ttcactaccg ctttcagcga atatgctctg gatgggtaca aggtcaatgc tttggattat
     3781 ctactgaaac ctttctccta ctccgaattt ttaagtgctt cgaaaaaggc attggaatgg
     3841 tttgaaatga aagccgcttc ggaggagaag ggagaatctg aagtttcggg aattttcgtc
     3901 cgctccgact ataagctggt gcatgtcctt tttgatgaca tcctattggt tgaggggttg
     3961 aaagattata ttaaaatttt caccggtaaa gatcccaagc ccatccttac gctcatgagt
     4021 atgaaatcga tggaagatga attgccagct acacggttta ttcgtgtaca tcggtcgttt
     4081 atcgtgaatc gcgataagat tgtccgggta gaaaaaaacc gcattatcat cggaaagcac
     4141 gaaatcccta tcggtgaaac ttaccgcaaa gcgtttatgg aagaaattaa tgggaagtaa
     4201 ataaaaaata ttctattcca atcgccgctt ataaatttca aaattctctt catcaaaaca
     4261 cacgaaagta acttcttcca caaatttagc cggaaaattt ttcactgcat ccacagctat
     4321 ttcagctgct ctttccttcg gaaaccggta aaccccggta ctgatgttgg gaaaagctat
     4381 cgatttcaat ccttgctcat cggcaagttt aagtgaattt acataacact ccttcagcag
     4441 actctcttcg tttcg
//
LOCUS       DAOSDR010000267         4450 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrimonas sp. isolate AMR_MDS_1826
            NODE_6895_length_4450_cov_3.492833, whole genome shotgun sequence.
ACCESSION   DAOSDR010000267 DAOSDR010000000
VERSION     DAOSDR010000267.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863355
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrimonas sp. (wastewater metagenome)
  ORGANISM  Petrimonas sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Dysgonomonadaceae; Petrimonas.
REFERENCE   1  (bases 1 to 4450)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4450)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:10:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,720
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 35
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4450
                     /organism="Petrimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6895_length_4450_cov_3.492833"
                     /isolate="AMR_MDS_1826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2023866"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..145)
                     /locus_tag="PLI30_10160"
     CDS             complement(<1..145)
                     /locus_tag="PLI30_10160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015531961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="THUMP domain-containing protein"
                     /protein_id="HOI79955.1"
                     /translation="MIQSENYPMIAKTMAELEDVLAEELITLGANDVEIGTRMVSFTG
                     DKAL"
     gene            complement(184..1707)
                     /locus_tag="PLI30_10165"
     CDS             complement(184..1707)
                     /locus_tag="PLI30_10165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009860003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 10 glycosylhydrolase"
                     /protein_id="HOI79956.1"
                     /translation="MNYFRITLIAFTALLVLSCGASRQSLTRTVGTAPKREFRGVWVQ
                     TVGQSRYQQMNSAAMKHYLSEMVRKFDEAGINALIFQVRPEADTFYPSDLEPWSRFLT
                     GVQGKSPDDPSFDPLAFLIEECHKRGMELHAWLNPYRVKTNINNPLAPSHIYWKYPER
                     FVQYGNQLFFDPGLPENRSFICQVVRDIVSRYDVDAVHMDDYFYPYPIAGTPFPDDNS
                     FNAYAGSQGFSAGQRADWRRNNVNLLIQQLKYTIAGTKPWVRFGVSPFGIYRNKRNTP
                     DGSGSDTNGLENYGDLYADIKLWVEKGWIDYNLPQLYWEIGHERADYTTLLQWWNANN
                     FGQPLYIGQDLQRSMDKNELDEKIRQSREMSFVHGSCFWYGYLILENSGGIADALKAG
                     AHRAKALIPAYTHLHKGRPVKVKKLTQIFTEDMHFLAWEHRRQPQNPETARQFVIYRF
                     SANEKVDLDRAENIVAVTPDNFYVLPYEGGGNKYTYAVTALDAFKNEGKAAKIKVKL"
     gene            complement(1711..3792)
                     /locus_tag="PLI30_10170"
     CDS             complement(1711..3792)
                     /locus_tag="PLI30_10170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019538035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HOI79957.1"
                     /translation="MNDFFSDITHEFTLPFTNPVLIFAVLLAIVLLAPILLKRFNVPS
                     IIGLIVAGVIIGPFGLNLIDNNHPGVSMFSTIGLLYIMFIVGLELDLNEFVANKNKSL
                     VFGLFTFILPLSIGFPVCHYLLGYDINASFLTASMFATHTLVTYPIVSRMGVAKNQAV
                     AITVGGTILTDTAVLIILALILSSSDGGLNLIFLLKLLVSLIVFSAIVFLLIPRITKW
                     FFQKAEGEKYLHYIFVLFVVFFCGFLAELGGIEPIIGAFAAGLALNRLIPHSSALMNR
                     IEFFGNSLFIPIFLISVGMLVNISVVFNGLTTVLIALALTVTAVFGKWVAAFFTQVIF
                     KYSVTQRNIIFGLSSSHAAATIAVIIVGYNAGILDEYILNGTIILILLTCIVASLVTQ
                     KSAKELAISQENAPFAESDLGAFAQEKILVPIANPSNIGHHVELALLMKDKRSVNAVS
                     LLGVVPNNEEAEKNIVSFRKQLKEFVQNAVAAEIDVDIVTTIDHNAASGIVRTARELM
                     TDIVILGWPGKTGVLEKLLGDKVDLIIKNVDKNMFICHIEQELITHKRIVVISPPLVE
                     RELGFDLWLQKVVKLSQELSVPVLHLGHPDTQAVISSKKNGGAPFIFKQFVDWHDPLS
                     CGDNIREDDMIIFVSAHQGYLSHMSILDHLPTRLEERFPHHSRIVIYPKQRVVEGLLE
                     SDDSLFVPSNF"
     gene            3890..>4450
                     /locus_tag="PLI30_10175"
     CDS             3890..>4450
                     /locus_tag="PLI30_10175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016266970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit A"
                     /protein_id="HOI79958.1"
                     /translation="MSSEKQIELKGVRVNNLKNIDVIIPRNAFTVITGLSGSGKSSLA
                     FDTLYAEGQRRYVESLSAYARQFLGRMNKPECDFIKGIPPAIAIEQKVNTRNPRSTVG
                     TSTEIYEYLRLLFARIGKTISPVSGDEVKKHTVSDVIAKMLQYREGTRMAVLSPIQLR
                     NGRTLPEQLDVLMKEGFSRVDVGDTFY"
BASE COUNT         1278 a         1055 c          972 g         1145 t
ORIGIN      
        1 tcaatgcctt gtcgccggta aaggagacca ttcttgtgcc aatttccaca tcgttggcac
       61 ccaaagtgat cagctcttca gccagtacgt cttcgagctc tgccatggtc ttggcaatca
      121 tgggataatt ttccgattga atcattcttt tgttttcaat aaattaacat aaccaatttc
      181 acatcaaagc ttaaccttga tctttgccgc ttttccttcg ttcttaaacg catctaatgc
      241 cgtcaccgca taagtatatt tgtttcctcc tccttcgtag ggcaacacgt agaaattgtc
      301 gggagttacc gctacaatat tttcggcccg atcgaggtct actttttcgt tggcactgaa
      361 ccgataaata acaaactgcc tggcggtttc gggattttgg ggctgtcttc ggtgctccca
      421 ggcaagaaag tgcatatctt cggtaaaaat ctgcgtaagt tttttcactt tgacaggacg
      481 tcctttgtgt aggtgagtat aggccggaat aagcgctttg gcacgatgag cccctgcttt
      541 gagcgcatcg gcaattcctc cggaattttc cagaataagg taaccgtacc agaagcagct
      601 accgtgtaca aatgacattt cacgcgattg acgaatcttt tcatccagtt catttttatc
      661 catgctgcgc tgcaaatctt ggccgatata gagtggttga ccgaagttgt tggcattcca
      721 ccattgaagc aacgtggtgt agtcggcacg ttcgtgtccg atttcccaat aaagttgcgg
      781 gagattataa tcgatccatc ctttttccac ccacagcttg atatcggcat agaggtctcc
      841 gtagttttcc aatccattgg tatcgcttcc ggagccgtcg ggtgtgtttc gcttgttgcg
      901 gtaaataccg aacgggctaa caccgaaacg tacccacggt tttgttcccg caatggtgta
      961 tttgagttgt tggataagta gattcacgtt gtttctcctc caatcggcac gctgtcccgc
     1021 agaaaatccc tgagaaccgg catacgcatt gaaactgtta tcatcaggaa aaggagttcc
     1081 ggcaatggga tagggataaa aatagtcgtc catatgaaca gcatccacat cataccggga
     1141 aacgatatcg cgaacaacct ggcaaataaa gctccggttc tccggcaacc cgggatcaaa
     1201 aaaaagttga tttccgtatt ggacaaatcg ttcaggatac ttccagtaga tatggctggg
     1261 agcaagcggg ttattgatat ttgtttttac ccggtaaggg tttagccaag catgcagctc
     1321 cattccccgt ttatgacact cttctatgag gaaagccagg gggtcgaaac tgggatcatc
     1381 gggagattta ccttgtacac ccgtcaagaa acggctccag ggttcgagat ccgacggata
     1441 gaaagtatcc gcttccggac gaacctggaa aataagtgcg ttgatacccg cttcgtcaaa
     1501 cttacgtacc atttcggaca aatagtgttt catggctgca ctgttcatct gctgataacg
     1561 ggattgcccg acggtttgca cccaaacccc tcgaaactcg cgcttgggag cagtgcccac
     1621 agtgcgggtc aacgattgtc ttgatgctcc acaacttaat accaggagcg ctgtaaaagc
     1681 gattaacgta attcgaaaat agttcattac ttaaaaatta gagggaacaa acaatgagtc
     1741 gtcactttcc agcaaccctt caactacccg ttgtttagga taaataacaa tacggctgtg
     1801 gtggggaaaa cgctcttcga gacgggtagg taaatgatca agtatggaca tgtgtgaaag
     1861 gtatccctgg tgtgccgata cgaaaataat catatcgtct tccctgatat tgtcgccaca
     1921 ggaaagcgga tcgtgccaat cgacaaactg tttgaagatg aagggggctc caccgttttt
     1981 cttgcttgat atcacggctt gtgtatcggg atgtccaagg tgcagaacgg ggacagacaa
     2041 ctcctgagac agcttcacca ctttttgaag ccataagtcg aatccgagtt ctctttccac
     2101 aagcggcgga gaaatcacta caatgcgctt gtgagtaatc aactcctgtt cgatgtggca
     2161 tataaacatg ttcttatcca cattcttgat gatcaaatct actttatctc ccaacagttt
     2221 ttccagcact cctgtttttc caggccaacc caaaatcact atatccgtca tcaactcacg
     2281 ggcagtacga acaattccgc ttgccgcatt atggtcaatg gtagtaacaa tgtccacatc
     2341 aatttcagca gccacggcgt tttgtacgaa ttccttcaac tgtttgcgaa aactcacaat
     2401 atttttttcg gcctcttcat tattgggcac cacgcctagc aatgaaaccg cattcaccga
     2461 tctcttgtct ttcatcagca aagcaagttc cacgtggtgt ccaatatttg atggattagc
     2521 aatgggtacc agaattttct cctgcgcaaa agcgcctaag tcggattcgg caaacggagc
     2581 attctcctgg ctgatagcaa gttctttggc cgatttctgc gtaacaagcg aagcaacaat
     2641 acaagtcagt aagatcagaa taatcgttcc gttcaaaata tattcatcca ggatacccgc
     2701 attatatcct acaataataa ccgcgatggt agctgcagca tgagaactgc ttaatccgaa
     2761 gataatgttc cgctgtgtta ccgagtactt aaagataacc tgcgtaaaaa aagcggctac
     2821 ccattttccg aaaacagcag taacggtaag tgccaaagca attaacacgg ttgttaatcc
     2881 attgaatact accgatatat tcaccaacat ccctacggag atcaaaaaga taggaataaa
     2941 caatgagttt ccgaaaaact ctatccggtt catcaatgcc gatgaatgcg gaatcaacct
     3001 gttcagagcc agtcccgcag caaaagcacc aataatcggc tcaattcccc cgagttccgc
     3061 caaaaagccg cagaagaaca ccacaaaaag tacaaaaatg taatgcaggt atttctcccc
     3121 ttcggctttc tggaaaaacc atttggtaat tcgcggaatg agcaggaaaa caatagcgga
     3181 aaaaacgatc agcgacacca gcaatttcag caggaagatc agatttagcc cgccatcact
     3241 gctggataag ataagtgcaa gaataatcaa aacggccgta tcagtcagga tggtacctcc
     3301 gaccgtaatg gcaaccgcct gatttttagc gacccccatt ctgctcacga taggataggt
     3361 gaccaatgtg tgcgtagcaa acatactggc cgtaaggaaa ctagcgttta tgtcgtaccc
     3421 cagcaggtag tggcataccg ggaatccaat ggaaagcgga aggataaagg tgaagagacc
     3481 aaagacaagg cttttgtttt tgttggcgac aaactcgttc aaatcaagtt ccaaccctac
     3541 gataaacata atgtaaagca atccgatcgt cgagaacatc gacacacccg ggtgattatt
     3601 atcgataaga ttcagtccga acgggccaat gatcactccc gcaacaataa gtccgataat
     3661 actcggcaca ttgaagcgtt tcagcaaaat cggagccagc aaaacaatag ccagcaacac
     3721 cgcaaagata agaacgggat tggtaaacgg cagtgtaaat tcgtgcgtta tatccgaaaa
     3781 aaaatcgttc atcggtggtg ttattctgat tcttccaaat ttcgtgattt gcaaaaatac
     3841 ggaaaatatt tcaataaagt cgttatcttt gcaggttgaa ttccaattta tgagcagcga
     3901 gaagcaaata gaactaaaag gggtacgcgt caataatctc aaaaacatcg acgtaattat
     3961 tccccggaat gcatttaccg ttatcacggg tctctccggt tcagggaaat catccctggc
     4021 attcgatacg ctttatgccg agggccagcg ccgctatgta gaaagcctct cggcttacgc
     4081 ccgccagttt cttggcagga tgaacaaacc cgagtgtgat tttattaaag gaattccacc
     4141 ggccatagcc attgaacaaa aagtaaacac ccgaaatccc cgctcaaccg taggtacctc
     4201 taccgaaatt tatgaatacc tgcggctact cttcgcccgg atcggtaaaa caatttcgcc
     4261 cgtttccggt gatgaggtaa aaaagcacac tgtcagcgat gtcattgcca aaatgcttca
     4321 ataccgggaa ggaacacgta tggcggtgct ttcgcccata caactgcgaa acggaagaac
     4381 gttaccggag caactggacg ttttaatgaa agagggcttt tcgcgtgtgg atgtgggcga
     4441 taccttttat
//
LOCUS       DAOSDR010000268         4370 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrimonas sp. isolate AMR_MDS_1826
            NODE_7062_length_4370_cov_3.722132, whole genome shotgun sequence.
ACCESSION   DAOSDR010000268 DAOSDR010000000
VERSION     DAOSDR010000268.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863355
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrimonas sp. (wastewater metagenome)
  ORGANISM  Petrimonas sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Dysgonomonadaceae; Petrimonas.
REFERENCE   1  (bases 1 to 4370)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4370)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:10:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,720
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 35
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4370
                     /organism="Petrimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7062_length_4370_cov_3.722132"
                     /isolate="AMR_MDS_1826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2023866"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..693
                     /locus_tag="PLI30_10180"
     CDS             <1..693
                     /locus_tag="PLI30_10180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005818591.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HOI79959.1"
                     /translation="YLAYFQSYTNTYDNVEKLIEMYEEALAYPDVVGIIIGTRPDCMP
                     DELLDYFAELNKKTFVLIEYGVESTLNKTLERVNRQHTWEESVEAITRTAEREVPVGV
                     HLILGLPGETDEDILSHAVEISKLPLTTVKLHQLQIIRGTAMAKEFRLLPESFHLFGL
                     EEYVDLCVDFAERLNPDFYIERFASQSPKSLLIAPDWGLKNYELTEKVKKRFKGKNTF
                     QGKLHSHKNHYK"
     gene            760..2847
                     /locus_tag="PLI30_10185"
     CDS             760..2847
                     /locus_tag="PLI30_10185"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOI79960.1"
                     /translation="MHFRKSFSLQKSGILFLFLYLIIPISAIEKDSLRHYNLDTLVVS
                     TFKHENNKLMRPMAASVVTSQLMERQQISEMKGFTGIIPNFMIIDRDTRLTSSVFIRG
                     VGALINTPGVAMYVDGIPHFEKSSFDINLLDIDKVEFLRGPQGTLYGRNAMGGTILVH
                     TRSPFRYQGTRLQLRYGSYDETSVTLSHLGKANDKFAHSIAGNYNYYGGHITNVHTGK
                     KANYLGSASVNTRFEWRPLPQMNIRLTNNFENTHQGAFSYGDVNNKTLLVDSVSTDHP
                     SYYKRKLYDGGLQINYYNDYIAIRSQTSVQHLNDTYEVDQDGSPKNLYFAIQSEKQRL
                     ISEEIILKGLDDNIYGWNFGVFAFNHGIDRATDVFMNLRTPKYKLEKRYDDYSRGIAL
                     FHQSTLNITSKLRVEAGLRYDYEKANSIHEEHKIIESEAQLINDFDSPLTFSQWTPKA
                     SLQYLPTHQSQFYLTVAKGYKTGGFNTVFDTPLQRTFEPEYSWNYELGSKGWIIPEKL
                     FAEVALFCINTYNQQIKQHLDQQGIKIFNAGSSVSQGFEATLNATPFKNFGIDVAYGF
                     THATFTKYQYSNDIDYSGKFLPFVPRNTVSVNADYTIHTTNEFSDEISFNANYTGIGV
                     MFWHESNTVQQPFYGLLNTSVTARNGNVSFSLWGKNITNTQYLGYFFEVGGRKRGKQG
                     KPLSVGISLTLNF"
     gene            2855..4102
                     /locus_tag="PLI30_10190"
     CDS             2855..4102
                     /locus_tag="PLI30_10190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018028855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOI79961.1"
                     /translation="MKIDKSYNLFTFFTLYIAQSIPMSFFSSVLPILMRQGDYSLVAI
                     ALLKLIKLPWIMKFLWSPIIDRKCDSINDYKRWILGSEIVYALLIFVVAMLNLQTNFT
                     LIIGLILLAFISSATQDIATDALAARSFERRDNSLLNSMQSMGSFTGSMVGGGFLLLL
                     FQQIGWGKMLPWVAIFVLLALVPLQLNINLRLRERQNKSKARPQDMYLFFKQKGIGKH
                     ALFLFLFYSGVIGILSILRPLMVDLGYDMKEIGAMIGVFGTATGIFCSFLSGLFIRKI
                     GREKSRRIISLLIVATASYFLWVHASGNVHSLVAIMAGIALVWGTYGMASTIVYTTSM
                     DIVREGREGTDFTMQIVITHLSSMIVAIICSRIGDIFGYDGLFALEISLAVISFVFVW
                     FYRPPFSMDKLEMEQIPNTSHNE"
     gene            4104..>4370
                     /locus_tag="PLI30_10195"
     CDS             4104..>4370
                     /locus_tag="PLI30_10195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010802295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="coproporphyrinogen III oxidase"
                     /protein_id="HOI79962.1"
                     /translation="MDHLISKYNTPVPRYTSYPPANFFHDNFAESQFWEAITHSNTQE
                     PNHLSFYIHIPFCKRMCYYCGCNSFPMQQEQEVREYRDAIKREIN"
BASE COUNT         1332 a          956 c          861 g         1221 t
ORIGIN      
        1 tacctggcat actttcaatc gtacaccaac acgtacgata acgtagaaaa actcatcgag
       61 atgtacgaag aagcgcttgc ctatcctgat gttgtgggca tcatcatagg cacccggccc
      121 gattgcatgc cggatgagtt gctggattat tttgcagagc tcaacaaaaa aacgttcgtt
      181 ttaatcgaat atggagtaga aagtacatta aataaaacgc tggagcgtgt aaaccgtcaa
      241 cacacctggg aggaatctgt tgaagccatt acaagaacag cagaaaggga agttccagta
      301 ggtgtacatt taattctggg gttaccggga gaaacagatg aagacattct aagtcacgcc
      361 gtagaaatct caaaattacc gctgacaacc gtaaaactcc accagttaca aattattaga
      421 gggacggcta tggccaaaga gttcaggttg cttccagagt cgtttcacct tttcggactt
      481 gaagagtatg tggatttatg cgttgatttc gcagaacggc tgaatcccga tttttatatt
      541 gaacgatttg cgtcccaatc accaaaatca ctgcttattg cacctgattg gggattgaaa
      601 aattatgagt tgacagaaaa agtaaagaaa cgatttaagg ggaaaaatac gtttcaggga
      661 aaactccact cccataagaa tcactacaag tagtgatttt ttttttaaca aaaacgcatt
      721 tactttgtac gttgaattta aaatttatac acaaatcaaa tgcactttag aaaatcgttt
      781 tcactacaaa aaagcggtat tttattcctt tttctttacc ttatcattcc aatttctgct
      841 attgaaaaag actccctacg acattacaac ttagatacgt tggtggtttc cacattcaag
      901 catgaaaaca acaaactcat gaggccaatg gcggcaagcg tggttacctc acaacttatg
      961 gagcggcaac agatcagcga aatgaaaggt tttaccggaa ttattcccaa ctttatgatc
     1021 atcgaccgcg acacacgcct cacctcctcg gttttcatca gaggtgttgg tgcgcttata
     1081 aacactcccg gcgtagcgat gtatgttgat ggaattccac attttgaaaa atcatcgttc
     1141 gatatcaatt tgttggatat tgacaaagtg gagttcctgc gaggaccaca aggcacactt
     1201 tacggcagaa atgccatggg gggcaccatt ctcgtacata ctcgttctcc tttccgctat
     1261 caaggcaccc gtcttcaact tcgctacggg agttacgacg aaacttccgt tacgctttct
     1321 catctcggca aagcgaacga caaattcgcc cacagcattg ccggaaacta caattactac
     1381 ggcggtcaca tcacaaatgt tcatacgggg aaaaaggcca attatctagg ttcagcttct
     1441 gtaaatacac gctttgaatg gcggccttta ccacaaatga atatccgcct aactaataat
     1501 ttcgagaata ctcaccaagg tgctttttct tatggagatg taaataacaa aacacttctc
     1561 gttgattctg taagcacaga tcatcccagc tattataaac gaaaactata cgatggaggc
     1621 cttcagataa actattacaa cgattacatc gccatcagat cgcaaacctc cgttcagcac
     1681 ttaaacgata cgtatgaagt tgaccaggac ggctcaccca aaaatctgta ttttgccatt
     1741 caatcagaaa aacaacgact aatatccgaa gaaatcatct tgaaaggact tgacgacaat
     1801 atttacggat ggaattttgg cgtgtttgct ttcaaccacg gtatcgatcg tgctaccgat
     1861 gtatttatga acttgaggac cccaaagtat aaactcgaaa aaagatacga tgattatagc
     1921 cggggaattg ccctgtttca tcaatccaca ctcaacatta cgtctaaact ccgtgttgaa
     1981 gccggtttac ggtatgatta tgagaaagca aattctattc acgaagaaca taaaataatc
     2041 gagagtgaag cacaattaat taacgatttc gattcgccac ttaccttttc acaatggaca
     2101 ccgaaagcat cactccaata tttacccact catcaatcac aattctatct taccgttgca
     2161 aaaggctata aaacaggcgg atttaatacc gttttcgata cacccctgca acggacattc
     2221 gaacccgaat acagttggaa ctatgaactg ggaagcaagg gttggatcat acccgaaaaa
     2281 ctttttgcag aggtagctct gttttgcatc aacacctaca atcaacaaat taagcaacac
     2341 ctggaccaac aaggaataaa aatatttaac gccggtagtt ctgtaagcca aggatttgaa
     2401 gctacactta acgcaacccc ttttaaaaac ttcgggatag atgtcgcata cggctttacg
     2461 catgcaacgt ttacgaaata tcaatacagt aatgatatcg attatagcgg taagttttta
     2521 ccttttgtcc cgcgtaatac agtttcggta aacgctgact atacgatcca cacaaccaac
     2581 gaattctccg atgaaataag ttttaacgca aactataccg gtattggtgt aatgttctgg
     2641 cacgagagca ataccgtaca acaaccgttc tatggcttac ttaatacaag cgtaaccgca
     2701 agaaacggca atgtaagctt ttcactctgg ggaaaaaaca tcaccaacac tcaatattta
     2761 ggttactttt ttgaggtagg cggacgaaaa cgtggaaaac aaggcaagcc tttatccgtt
     2821 gggatcagct tgactttaaa cttctgaagc aaaaatgaaa atagataaat cgtacaatct
     2881 ctttaccttt ttcacactct atatcgccca atccattccg atgagctttt tctcttcggt
     2941 tttacctatc ttaatgcggc aaggcgacta ttctctcgtg gccatcgcgc tgctgaaact
     3001 aataaagttg ccgtggatca tgaaatttct gtggtcaccg ataatcgata gaaaatgcga
     3061 tagcataaac gattacaagc gatggatttt gggttcggaa atagtctacg ccctgctcat
     3121 ttttgttgtg gctatgctca acctgcaaac aaatttcacg ctcatcatcg ggttgattct
     3181 tcttgcattt atttcatcgg ctactcagga tattgccaca gatgcgttgg ctgccaggtc
     3241 ttttgaacgc cgcgataaca gcttgctcaa cagcatgcag tctatgggtt cgtttacagg
     3301 aagcatggta ggcgggggat tcctcctact gctctttcag caaatcggat ggggtaaaat
     3361 gctaccgtgg gttgccatat ttgtattact ggcgcttgtt ccactgcaat taaatataaa
     3421 cctaagatta cgcgaacggc aaaataaaag taaggctcgc ccacaagaca tgtacctgtt
     3481 ctttaaacag aaaggaattg gcaaacacgc gttattcctc tttttgtttt attcgggagt
     3541 gatcggcatt ctctccattc tccggccgtt gatggtagat ctgggctacg atatgaaaga
     3601 aatcggggca atgataggtg ttttcggaac ggctaccggc atcttctgtt cgttcctttc
     3661 ggggctgttt atccgaaaga tcgggcgaga aaaatcacga cgaatcatat cgctgcttat
     3721 cgttgccacg gcaagctatt tcctgtgggt gcacgcttcg ggaaacgtcc acagtctcgt
     3781 ggcaataatg gcaggaattg ctctcgtatg gggcacttac ggtatggctt ccactatagt
     3841 ttataccaca tcgatggaca ttgtgcgaga aggcagggaa ggtactgatt ttactatgca
     3901 aattgttatt acgcatctaa gttctatgat cgttgccatt atttgttcac gaattggaga
     3961 tattttcgga tacgacggat tattcgcatt agagatttct ctggcagtaa tttcgttcgt
     4021 tttcgtgtgg ttttatcgtc ctcctttttc aatggataaa cttgaaatgg aacaaattcc
     4081 aaatacttca cataacgaat aaaatggatc atttaatttc aaaatacaat actcctgtac
     4141 cccgctatac cagttatccg cctgcaaatt tctttcacga taattttgca gagtctcaat
     4201 tttgggaagc tattacacac tccaacactc aagaacccaa tcacctctct ttctacatcc
     4261 atatcccgtt ttgcaaacga atgtgctatt attgcggatg caactctttt cctatgcagc
     4321 aagagcaaga ggtaagagaa tacagggacg ccataaaaag agaaataaat
//
LOCUS       DAOSDR010000269         4345 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrimonas sp. isolate AMR_MDS_1826
            NODE_7108_length_4345_cov_3.827739, whole genome shotgun sequence.
ACCESSION   DAOSDR010000269 DAOSDR010000000
VERSION     DAOSDR010000269.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863355
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrimonas sp. (wastewater metagenome)
  ORGANISM  Petrimonas sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Dysgonomonadaceae; Petrimonas.
REFERENCE   1  (bases 1 to 4345)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4345)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:10:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,720
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 35
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4345
                     /organism="Petrimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7108_length_4345_cov_3.827739"
                     /isolate="AMR_MDS_1826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2023866"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..590
                     /locus_tag="PLI30_10200"
     CDS             <1..590
                     /locus_tag="PLI30_10200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009275898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1080 domain-containing protein"
                     /protein_id="HOI79963.1"
                     /translation="RQYNGNSVPENWIIEDGTMEVFTAPNARPGQGVGGDLIYFASKF
                     KNFELSIDWKAEKEANSGIFYNVREEKGRAIYSSAPEVQILDNENASDNKIDSHLAGS
                     LYDMLPADPKTVNPYGEWNTIVIRVDNGKVTHTQNGVKVVEYRLWTKDWDRMVENSKF
                     KSFPGFQEGVSREGYIGLQDHGYAIWFRNVKIREL"
     gene            complement(663..2204)
                     /locus_tag="PLI30_10205"
     CDS             complement(663..2204)
                     /locus_tag="PLI30_10205"
                     /inference="COORDINATES: protein motif:HMM:NF024181.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SusD/RagB family nutrient-binding outer membrane
                     lipoprotein"
                     /protein_id="HOI79964.1"
                     /translation="MNAKLFKITATLLVAVFIGSCSNFETINTNPDQPSVASAPWLAT
                     NLITTVTSSATSTTKGFTEPYQQAKYLLFTERKEGTQYNYMGRANFSSRLTVLKNVNP
                     MIEYAKKLSPELANSYEALGHFIRAWQFFHLTMSVGDIPYSEAIQGNEGIIKPKYDTQ
                     KDVFKGILNELDQANDLFSKGTDISGDFIYKGNVEKWRRLVNSFELYVLINLYKKTSD
                     NDLNVIAKFKEVAQRPLMRDFNDNFAITYNTSKGYCYPWSNTPTDLNPFTQYTMLSTT
                     YIDLLKQNQDRRLFYVAEPAESLLAQGKIASNFDAYIGVEPSDEYNSTIDKKNMNEYS
                     DLNKRYVESATAEPVGLLCHWDLEFILAEAAVRGWIDSDAQTHYANGIRSSMRFYAHY
                     VPDNYTHNVSMDDPYIASYPASVALAGNTENKIKQIISQKYMAGFFHNGYLAWFEFRR
                     TGYPEFVLNPNSNMNPTDNTKFPTRWQYPQNEINYNTDNLNEAVQRQYNGSDDFNGIM
                     WILKE"
     gene            complement(2228..>4345)
                     /locus_tag="PLI30_10210"
     CDS             complement(2228..>4345)
                     /locus_tag="PLI30_10210"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOI79965.1"
                     /translation="GENGYFRTGLNYVYNKGQYPNQTLNKFNYSLSGEIKLGDKLLVE
                     SQMGYMRSTAPQIWGGGYGNQGYIYQILMWTGPDYDIRQYRDYWVTPNEKQNWLYNAW
                     YDNPYLIAYEKLLGVEENKLNASLTVNYNILDNLKVIFRNGYDFYKNEDKLRNPAGIY
                     STRGPSVSGNYWGWNGKGMYGMNQRWGHSINSDLMLTYNKSFGKFDFDLLTGGSLYYY
                     QDRYQGARTANGLAVPGWYSLANAVPSTAAGVNSIENIVGTYAREVNSIYGKLSLAWN
                     NAIYVDLTGRNDWSSTQPKEEMSYFYPSVVGSVVLSEFFQAPSWLSMWKIRGSWTIAK
                     SPLGVYENNLPYSTYNSWGYIGASLAANLKGTDLLPSETRTWETGTAAYFFGKRLYLD
                     IAYFDKLYYNRQIKQDIAPSSGFNSTLINTEETYARRGVEITVSGSIIKNKEFEWNSM
                     INYSNQHRYYVDIDPVYSQKDQWTKSGKRLDYYAQTEKVLRDPEGNMIHSADGNVWLN
                     NYRQLYGYRDPNFTFGFINNFTWRNLTMGISLDGRIGGLMDNYIYGKMFDTGSAPETD
                     TQDRYDEVVNGKKYIGKGVKVISGTAKYDTDGNIIEDTRQYASNDIPVSYQEYMRLWG
                     NSWEERIRNQTFIKLREVSIAYSFPKKLLGKTFLNQASIALTGQNLLLWTKDFKYSDP
                     DVGSEDMNAPSQRMIGFDLKIGF"
BASE COUNT         1143 a          912 c          925 g         1365 t
ORIGIN      
        1 ggcgccagta taacgggaac agtgtgcctg agaattggat catcgaagac ggtaccatgg
       61 aagtgtttac ggccccgaat gcacgtccgg ggcaaggggt aggaggcgat ctgatttact
      121 tcgccagtaa attcaaaaac tttgaactct ccattgactg gaaggccgaa aaagaggcca
      181 actccggtat tttctacaac gtccgcgaag aaaaagggag ggccatttac agctcggcgc
      241 ccgaagtcca aattctggat aacgagaatg ctagcgacaa caaaatagac agtcacctcg
      301 ccggatcgct ttacgatatg ctgcctgcgg atccaaagac cgtgaaccct tacggggaat
      361 ggaacacgat tgttattcgg gtggacaacg gcaaggttac ccatacgcaa aacggggtga
      421 aagtagttga ataccgcctg tggacaaaag attgggacag aatggtggaa aacagcaagt
      481 tcaaaagttt tcccggattc caggaaggtg tttcccggga aggttatatc gggttgcaag
      541 atcacggata tgccatctgg ttccgtaacg tgaaaatcag ggagttgtaa agattaacca
      601 agtaaaaaat caaaaggcgt ttacgaaagt agtaaacgcc ttttggtttt tattaggtga
      661 agctattctt ttaatatcca catgataccg ttgaagtcgt cactgccgtt atattgtcgt
      721 tgaacggctt cattgaggtt gtcggtattg tagttaatct cgttttgtgg atattgccac
      781 ctcgttggaa attttgtatt atccgtcgga ttcatatttg agttaggatt taaaacaaat
      841 tcaggataac ctgtacgtct gaactcaaac catgccaagt agccattatg aaagaatccc
      901 gccatgtatt tctggctgat tatttgtttg attttgtttt ccgtatttcc cgcaagtgct
      961 acagaagcag gataagatgc aatgtatgga tcgtccatac taacgttgtg tgtgtagtta
     1021 tcaggcacgt agtgtgcgta gaatctcatg gaactcctaa tcccgttagc atagtgggtt
     1081 tgggcatcac tgtcaatcca tcctcttact gcggcttcag ctaaaataaa ttcaagatcc
     1141 cagtggcaca atagccctac cggttccgct gtggccgatt ctacgtatct cttatttaga
     1201 tcgctgtatt cattcatgtt tttcttgtca atggtgctgt tgtattcatc tgatggctcc
     1261 actccaatat atgcatcaaa gttcgaagca atcttgcctt gtgctaggag tgactcggca
     1321 ggttctgcca cgtaaaacag tcgacggtcc tgattctgtt tcaataagtc tatataggtt
     1381 gtggaaagca tggtgtattg agtgaacgga ttcaggtctg tgggagtgtt gctccacgga
     1441 taacaatatc ctttggaggt attgtatgtt atggcaaaat tatcgttaaa gtctctcatt
     1501 aacggacgtt gtgccacttc cttgaatttg gcaatgacgt ttaagtcgtt gtcggaggtt
     1561 tttttataca ggttaatcaa tacatacaac tcaaagctat taactagacg acgccacttt
     1621 tcgacattgc ctttgtaaat aaaatcgccc gatatgtcag ttcctttgga gaacaagtcg
     1681 ttggcctgat ccaactcatt taggattcct ttgaatacat ctttttgagt atcgtatttg
     1741 ggtttgataa ttccttcatt gccctggata gcctctgaat acggaatatc tcctactgac
     1801 atggtgagat gaaaaaactg ccaggcacgg ataaaatggc ccaatgcttc gtatgaattt
     1861 gctaattccg ggcttagttt tttagcatat tcaatcatgg gatttacatt ctttaggacg
     1921 gtcaacctgc tggaaaaatt tgcccttccc atatagttgt attgtgtacc ttctttcctt
     1981 tcggtaaaaa gtaagtattt ggcttgctga taaggttccg taaatccttt tgtggtagac
     2041 gtagcggacg acgtcactgt tgttatcaga ttggtggcca gccagggtgc agatgctact
     2101 gaaggttggt cgggatttgt attaattgtt tcaaaattac tgcaactgcc gatgaaaact
     2161 gcaaccaaca aagtagctgt tattttgaat agttttgcat tcataattat aaagtttatt
     2221 tgattaatta aaagccaatt ttaagatcaa aaccgatcat acgctgtgag ggggcattca
     2281 tgtcttctga acctacatcc ggatcggaat atttaaaatc cttagtccag agaagcaaat
     2341 tttgtccggt aagcgctata gaagcttgat tgagaaaggt cttgccaagt aattttttgg
     2401 gaaaactgta agcaatagat acttccctta acttaatgaa cgtttggttg cgaatacgtt
     2461 cttcccagga attaccccac aacctcatat attcctggta gctcaccgga atgtcgttgg
     2521 aggcatactg ccgtgtatct tcaataatat tgccatcggt atcatatttt gctgtgccgc
     2581 tgatgacttt tacccctttc cctatgtatt ttttcccgtt taccacttca tcgtaacggt
     2641 cttgggtgtc tgtttccgga gccgatccag tatcaaacat cttgccatat atgtaattat
     2701 ccattaaccc ccctatgcgg ccatccaaac taatacccat agttaaattc cgccaggtga
     2761 aattattgat aaagccaaag gtaaagttgg gatctctgta tccgtacagt tgtctgtagt
     2821 tgtttaacca cacgtttcca tcagcactgt ggatcatatt gccttcagga tctctgagca
     2881 ccttttctgt ttgtgcgtaa tagtccagtc ttttgccgga tttggtccac tggtctttct
     2941 gcgagtatac cggatcgata tccacatagt acctgtgttg atttgaatag ttaatcatgg
     3001 agttccattc gaactccttg ttttttatga tgctgcccga taccgtaatt tcaacacctc
     3061 gcctggcata ggtttcttca gtattaatca gtgtgctgtt aaaaccggaa gatggagcaa
     3121 tgtcttgttt aatttgcctg ttataataaa gtttatcgaa ataggcaatg tccaggtata
     3181 gtcttttgcc aaagaagtac gcggccgtac cggtctccca ggtgcgggtt tccgaaggga
     3241 gaagatcggt tccttttaaa ttggcggcaa gcgaagctcc gatatatccc catgaattgt
     3301 atgtactgta aggcaggttg ttttcgtata cccccagtgg agatttggca attgtccatg
     3361 atcccctgat tttccacatg ctgagccatg aaggtgcctg aaaaaattca gacaatacaa
     3421 cgcttcctac aacggaagga tagaaatagg acatctcttc tttcggctgt gttgaactcc
     3481 agtcatttct tcctgttaag tctacataaa ttgcattgtt ccatgccagt gataactttc
     3541 cgtatatact gtttacttcg cgtgcatatg tgcctacaat attttctatg gaattaacac
     3601 ctgcagccgt ggagggtaca gcattggcca atgagtacca tccgggtacc gccagtccat
     3661 ttgccgtcct ggctccctga tacctgtcct ggtagtagta caggcttcct ccggtcaata
     3721 aatcaaaatc aaattttccg aaagatttgt tatatgtaag cattaagtcg ctattaatac
     3781 tgtgtcccca cctttggttc ataccgtaca tgcctttccc gttccagccc caataattgc
     3841 cgctgacgga tgggcctctg gtggaatata tgccggcagg atttcttaat ttatcttcat
     3901 tcttatagaa atcatatccg ttccggaaaa taacttttaa attatccagg atattgtagt
     3961 tcacggtaag gcttgcgttt aacttattct cttcaactcc cagcagtttc tcataggcaa
     4021 tcaaataagg attatcgtac catgcgttat aaagccagtt ttgtttttca ttgggcgtaa
     4081 cccaataatc cctgtattga cgaatgtcat aatcgggtcc tgtccacatc aatatctggt
     4141 aaatgtatcc ttgattacca tatccacctc cccaaatctg gggagccgtt gaacgcatat
     4201 agcccatctg gctttctacc agtaatttat ctcccaattt tatttcaccg ctaagtgaat
     4261 aattgaattt gttgagtgtt tggttgggat attgcccttt gttatacaca tagttaagtc
     4321 ctgttctaaa ataaccgttt tcccc
//
LOCUS       DAOSDR010000270         4326 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrimonas sp. isolate AMR_MDS_1826
            NODE_7158_length_4326_cov_3.687661, whole genome shotgun sequence.
ACCESSION   DAOSDR010000270 DAOSDR010000000
VERSION     DAOSDR010000270.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863355
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrimonas sp. (wastewater metagenome)
  ORGANISM  Petrimonas sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Dysgonomonadaceae; Petrimonas.
REFERENCE   1  (bases 1 to 4326)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4326)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:10:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,720
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 35
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4326
                     /organism="Petrimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7158_length_4326_cov_3.687661"
                     /isolate="AMR_MDS_1826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2023866"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..222
                     /locus_tag="PLI30_10215"
     CDS             <1..222
                     /locus_tag="PLI30_10215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004315773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HOI79966.1"
                     /translation="VVSLQNDATTSYKTYIAVQNELVKAYNELRQEGSKKYFNSDYEN
                     LTDDQKEQINKLYPQRISEAEPKNFMGGN"
     gene            224..682
                     /locus_tag="PLI30_10220"
     CDS             224..682
                     /locus_tag="PLI30_10220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005637276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HOI79967.1"
                     /translation="MGKFSKSGGREMPELNTASMPDLVFAILFFFMVTTTMRSEELMV
                     RLKLPSATEIQKLEKKSLVTYINIGPAMDPRFGTGTQMQLNDKFAEVEDIQDYIAQEK
                     ASMNEADQQLMTVSIKADEQTRMRYISDVKQALRKAYALKISYSARKAEN"
     gene            complement(716..1948)
                     /locus_tag="PLI30_10225"
     CDS             complement(716..1948)
                     /locus_tag="PLI30_10225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006745530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfatase-maturation protein"
                     /protein_id="HOI79968.1"
                     /translation="MSVFAPFAKPLYVMLKPVGSRCNLDCDYCYYLEKANLYTEKKNH
                     VMSEELLEKFIKEYIESQTMPQVLFTWHGGETLMRPLSFYKKAVELQKKYAGGRQIDN
                     SIQTNGTLLNDDWCRFFKDNNFLVGISIDGPQEFHDEYRRDKMGRPSFHKVMRGMELL
                     HKHGVEYNCMAVVNDYNADFPLEFYRFFKTTGSRFIQFTPIVERIRKKNSPLKLATAE
                     DRVDAVELAPFSVSPEKWGSFLCAIFDEWVKEDVGQVFVQLFDSTLANWVGEQPGLCT
                     MAKTCGHAGVMEFNGDVYSCDHFVFPEYRLGNIYAKPLASMMYSNEQLTFGNDKFDKL
                     PRQCRECDFLFACYGECPKNRFVRDKYGIPGLNYLCKGYYTFFNHVAPYMDFMKRELL
                     AERPPANVMEWIKKTGSR"
     gene            2033..2449
                     /locus_tag="PLI30_10230"
     CDS             2033..2449
                     /locus_tag="PLI30_10230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019537746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HOI79969.1"
                     /translation="MRKDKNLLQAGLLLLRVGIGVSIFFHGLPKITGGPETWTAIGGT
                     MSNLGINFAPTFWGFMAAFAETIGGILFTLGLFFRPAALLLIGTMTVALVMHFSQGDD
                     FMKYGHALDLLIVFAAALLTGPGKYSFDAKYIPRIA"
     gene            2683..3906
                     /gene="pepT"
                     /locus_tag="PLI30_10235"
     CDS             2683..3906
                     /gene="pepT"
                     /locus_tag="PLI30_10235"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007484620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045148 - tripeptide aminopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HOI79970.1"
                     /translation="MNIVERFIKYVKIDTQSDENSSQTPSTQKQFNLAKEVEREMLEM
                     GLTEVSLDNNCYLMGTLPSNTDEQVPTIGFIAHFDTSPDMSGENVNPRIVKNYDGKEI
                     VLNEAQQIISSPADFPELLKFIGDDLIVTDGTTLLGADDKAGIAEIMTAMEYLIDHPE
                     IKHGKIRVGFTPDEEIGRGADKFNVDKFGAEWAYTMDGSEIGELEYENFNAASAKISI
                     QGRNVHPGYAKNKMINALHVANELVQMLPVNGRPEYTEKYEGFFHLIAINGTVDEAAV
                     SYIIRDHDRQIFENRKKEMEQAVAFLNARYNNRLKLEMRDQYYNMREKVEPVKYVVDY
                     AFRAMEEANITPIVKPIRGGTDGARLSFMGLPCPNIFAGGMNFHGRHEFIPIRAMEKA
                     TEVIVKIAGLVAKEA"
     gene            3927..>4326
                     /locus_tag="PLI30_10240"
     CDS             3927..>4326
                     /locus_tag="PLI30_10240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007212859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HOI79971.1"
                     /translation="MKKTPFTDTHIALGAKMHEFAGYNMPIEYSGIIDEHLTVVNAVG
                     VFDVSHMGEFWVKGPKALEFVQKVTTNDASKLQVGKIQYTCFPNEQGGIKDDFLLYHF
                     EPEKYLMVVNAANIEKDWEWCQKQNTMGAVI"
BASE COUNT         1276 a          995 c         1018 g         1037 t
ORIGIN      
        1 gttgtgtcgt tacaaaatga cgctaccacc agttacaaaa cctatatcgc ggttcagaac
       61 gaactggtaa aagcgtataa cgagttgcgg caggaaggat cgaagaagta tttcaactca
      121 gactacgaaa atctgaccga cgatcaaaag gaacagataa ataaattgta tccgcagaga
      181 atttctgagg ccgaacctaa aaactttatg ggaggaaatt aatatgggaa aatttagtaa
      241 aagcggcggg cgtgagatgc ccgaattgaa cacagcttca atgcctgacc tggtatttgc
      301 catcttattc ttctttatgg ttacaaccac catgcgctct gaggagttaa tggtaaggtt
      361 gaaattgcct tctgccactg aaattcagaa actggagaaa aaatcgctgg taacctacat
      421 caatatcggg cctgcaatgg acccaagatt cggtacggga acccagatgc agctgaacga
      481 caagtttgcc gaagtggaag acatccagga ttatatcgcc caggaaaaag cgagtatgaa
      541 tgaggcggac cagcaattga tgacggtatc catcaaggcg gacgaacaaa caaggatgcg
      601 ctacatcagc gatgttaaac aggcactccg taaagcatat gctttgaaga tcagttattc
      661 ggcacgaaaa gctgaaaact aaggagcatc tttctacaat agaaaaaccg ggtcactatc
      721 gtgatccggt ttttttgatc cattccatca cattggccgg tggccgctca gccaggagtt
      781 cacgcttcat aaaatccatg tagggtgcaa cgtggttaaa gaatgtatag taacctttac
      841 aaaggtaatt taatccggga atcccatact tatctctcac aaatctattt ttggggcatt
      901 ctccgtaaca ggcaaaaaga aaatcacact ccctgcattg ccggggaagt ttatcgaact
      961 tgtcgttgcc aaaggtgagc tgctcgttgg aatacatcat tgaagcgagc ggcttggcgt
     1021 aaatattgcc caaacggtat tccgggaaaa caaaatggtc gcacgaatag acatccccgt
     1081 tgaactccat caccccggca tgtccgcaag tcttggccat tgtacaaagc ccgggttgct
     1141 ctcccaccca gttagccagc gtggagtcga aaagctgaac aaaaacctgc cccacatctt
     1201 ctttcaccca ttcgtcgaaa atagcgcaga ggaaactgcc ccacttctcc ggagaaacac
     1261 tgaaaggcgc caactccacc gcatccaccc tatcttctgc cgtggccagc tttagcggag
     1321 aattcttttt tcgtattcgt tcgacgatag gggtaaactg aatgaagcgg ctaccggtag
     1381 ttttgaaaaa acgataaaac tccaacggga aatcggcgtt gtaatcgttc accacagcca
     1441 tacagttata ttccactccg tgcttatgaa gaagctccat cccgcgcatt accttatgaa
     1501 acgacgggcg gcccatctta tccctccggt actcatcgtg aaactcctgc ggcccatcaa
     1561 tggagatacc caccaggaaa ttgttatcct tgaaaaagcg gcaccagtca tcgttaagca
     1621 acgttccgtt ggtttgaatg gaattatcaa tctgccgtcc tccggcatat tttttctgca
     1681 gttcgactgc ctttttgtag aaacttaacg ggcgcatcaa cgtttcaccg ccgtgccagg
     1741 taaacagcac ctgcggcatg gtctgcgact ctatgtattc tttgatgaac ttttcgagca
     1801 gttcttcact catcacgtgg ttcttcttct ctgtatacag gtttgccttc tcgaggtaat
     1861 agcaataatc acaatcgagg ttacaacgcg accctacggg tttgagcatc acatataacg
     1921 gtttggcaaa gggagcaaaa acagacatat caattttatt tattggacaa atataaacaa
     1981 tttgtagggt tttggagttt aggccttgaa tataatttaa aataatgaca acatgagaaa
     2041 agataaaaat ttattgcagg ccggattgct cctcctgcga gtaggtattg gcgtttcgat
     2101 atttttccac ggtttaccca aaattacggg aggcccggag acgtggacgg ccatcggcgg
     2161 caccatgtcg aacctgggca taaactttgc tcccacgttc tggggattta tggcggcctt
     2221 tgccgagact atcgggggaa ttttgtttac cctgggactg tttttccgtc cggcagccct
     2281 cttgcttatc ggaaccatga cagtagcgct ggttatgcac ttttcacagg gcgatgattt
     2341 tatgaaatac gggcatgcac tcgacttgct gattgttttt gcagcggctt tgctcacggg
     2401 ccccggaaaa tattccttcg atgcaaaata tatccctcgc attgcttaag gccctctgtc
     2461 ccgtgtaccg agcagaataa aaaaagggta agcttactat atcgcgccgc tacaaccaaa
     2521 tacccttgct tcggtcaaga cctgggggat tcaatgggag ctggccgtat aggacttacc
     2581 caactgcaaa gatacggttt tttttttagt tacaaaacgg ttgatttgat tttctgaaat
     2641 aaaaggcttt tgttatcttt gcggttacta aaagaaaaaa caatgaacat tgttgaacga
     2701 tttattaagt acgtaaagat tgatacccag tctgacgaaa atagttcgca aacccccagt
     2761 acacagaaac aatttaacct ggctaaagag gtggaacgcg aaatgctgga gatgggttta
     2821 acagaagttt ccctcgacaa caactgctac ctgatgggca ccctaccctc aaataccgat
     2881 gaacaagtgc ccactatcgg attcatcgca cactttgata caagtcccga catgagcggg
     2941 gaaaacgtaa atccgcgtat cgtcaaaaac tacgacggaa aagagatcgt actgaacgaa
     3001 gcacagcaga ttatctcctc gcctgccgac tttcccgagt tgttaaaatt tatcggggat
     3061 gacctgatcg taaccgacgg gacaaccttg ctcggcgcag acgacaaggc aggtattgcc
     3121 gagatcatga ccgctatgga atacctgatt gatcatcccg agatcaagca cggaaagata
     3181 cgtgtaggat tcacgcccga cgaagagatc ggccgcggag cggataaatt caacgtggat
     3241 aaatttggtg cagaatgggc ctatacgatg gatggcagcg agataggcga gctggaatat
     3301 gaaaacttca acgctgcatc ggcaaaaata tcgatccagg gaagaaatgt tcatcccgga
     3361 tatgccaaaa acaaaatgat aaacgcactg catgtggcca acgaactcgt gcagatgctg
     3421 cccgtcaatg gccgccccga atacacggaa aaatacgaag ggttcttcca cttgatcgca
     3481 atcaacggaa cggtggacga agcggcggta tcctatatta tccgcgatca cgacaggcag
     3541 atttttgaaa atcggaagaa agagatggag caggccgtag cgttcctaaa cgcccgttac
     3601 aacaaccggt tgaagctgga gatgcgcgac cagtactaca acatgcgtga aaaagtggag
     3661 ccggtaaagt atgtggtgga ttacgctttc agggctatgg aagaagcaaa cataacgccg
     3721 attgtaaagc ctatccgtgg aggaacggac ggagccagac tctcttttat gggcctgcct
     3781 tgcccgaata tttttgccgg aggcatgaat tttcacggcc gtcacgagtt tatacctatc
     3841 cgggcgatgg aaaaagcaac ggaggtcatc gtgaagattg ccggactggt agctaaagaa
     3901 gcataagttt taacacgcaa tatgatatga aaaaaacacc ttttacagac acacacatcg
     3961 cactgggtgc aaaaatgcat gaatttgcag ggtacaacat gcccatcgag tattcgggta
     4021 ttatcgatga acacctgaca gtcgtaaatg ccgtgggcgt tttcgacgtt tcacacatgg
     4081 gcgaattttg ggtgaaaggc cccaaagcgc tggaatttgt tcaaaaggta accaccaacg
     4141 acgcctcaaa gctgcaggtg gggaagatac aatacacctg ttttccaaac gagcagggtg
     4201 gcataaagga tgacttcctg ctttaccatt tcgaacccga aaaatactta atggtggtaa
     4261 acgccgccaa catcgaaaaa gactgggagt ggtgtcaaaa gcaaaacacg atgggtgcag
     4321 tgatcg
//
LOCUS       DAOSDR010000271         4283 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrimonas sp. isolate AMR_MDS_1826
            NODE_7258_length_4283_cov_3.381031, whole genome shotgun sequence.
ACCESSION   DAOSDR010000271 DAOSDR010000000
VERSION     DAOSDR010000271.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863355
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrimonas sp. (wastewater metagenome)
  ORGANISM  Petrimonas sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Dysgonomonadaceae; Petrimonas.
REFERENCE   1  (bases 1 to 4283)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4283)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:10:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,720
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 35
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4283
                     /organism="Petrimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7258_length_4283_cov_3.381031"
                     /isolate="AMR_MDS_1826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2023866"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..263)
                     /locus_tag="PLI30_10245"
     CDS             complement(<1..263)
                     /locus_tag="PLI30_10245"
                     /inference="COORDINATES: protein motif:HMM:NF024257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HOI79972.1"
                     /translation="MKQLVIIMMAVLVLYSCGNKSSKNESRKPASKITLTAAGATFPM
                     PYYNMVFSQYTQSTGTQLTYGGIGSGGGIRSLTDKVIDFGATDA"
     gene            complement(297..1283)
                     /locus_tag="PLI30_10250"
     CDS             complement(297..1283)
                     /locus_tag="PLI30_10250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI79973.1"
                     /translation="MKKSLSLLLLAILAALNTWGQTTTETFKPGGKPTALIFTNFNTA
                     FLDGESTPAFEITRAYLGYEYNFSQNWYGRLTVDVGDPKVGNLQYTAFLKFAYMQYKN
                     ENFKALFGMIPTTQFKYSEDIWGLRYIEKVFMDAYFLYLSADLGVTANYTFTKNISAD
                     LSVFNGEGYKSHQRDEFLRPALGITVKDLGSFTARIYADYMGKEVKQTSFAGFAAYAG
                     KKFVAAAEYNYQWNTKMIEGQDMYGTSVYTTWKPGEKIKYFARFDDLQSKPLKGQTTP
                     WQLAKDGQLLMGGLEFSPVKGIRVSPNIRYWNPADEAQKATTYAFINVEIRY"
     gene            1723..1851
                     /gene="kdpF"
                     /locus_tag="PLI30_10255"
     CDS             1723..1851
                     /gene="kdpF"
                     /locus_tag="PLI30_10255"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR02115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit F"
                     /protein_id="HOI79974.1"
                     /translation="MTTTILMAVQQTDNFLGYIFGATIALFLLGYLFYSLVKPEKF"
     gene            1855..3546
                     /gene="kdpA"
                     /locus_tag="PLI30_10260"
     CDS             1855..3546
                     /gene="kdpA"
                     /locus_tag="PLI30_10260"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpA"
                     /protein_id="HOI79975.1"
                     /translation="MTTQDAILLILFISILAGLTPVVGNYLFMVFTDGKHFMRPVLGW
                     LESVTYKFAGINPKEETNWKTYTSNLLLFNLIGIVFLFVIQLTQAYLPLNPAHLGNTS
                     WHLAFNTAVSFVTNTNWQSYAGETTQSYLVQMLGFTVQNFVSAATGMAVLLAVIRGIS
                     RKTTDKIGNFWTDLTRTTIYILLPASVVLAIVLAGQGVIQNFKTYETVQTVEGSLQIL
                     PMGPAASQIAIKLLGTNGGGFFNANSAHPFENPTPLSNFLQMLAIFIIPMGLTYTYGK
                     MVGSLRHGRTIFLTMGILFLLGLGISLYSEYSSNPIFGNLPLMEGKETRFGITNSVLF
                     SVVTTNASCGAVNAMHDSLSPISGMIALINMMLGEVIFGGVGAGLYGMIIFIILTVFI
                     AGLMVGRTPEYLGKKIEAFEVKMAIVAVLAPSLVILLFSAWASVSPVGLAGLNNPGPH
                     GLSEILYAYSSAAGNNGSAFAGLNANTIFYNLTTGIGMLIGRFGIIVPVLAIAGNMAQ
                     KRVTPLSSGTFRTDNWLFASLLTAVILIVGGLTFFPALSLGPVVEHLLMNSGLTF"
     gene            3557..>4283
                     /locus_tag="PLI30_10265"
     CDS             3557..>4283
                     /locus_tag="PLI30_10265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HOI79976.1"
                     /translation="MSTKPEIKLFNKRLLRQAVQESFVKLNPVLLIKNPVIFIVAIGS
                     VLTTLVVFMDIFNGSFSFFNFQIAVWLWFTVLFANFSEAVAEGRGKAQADNLRKNRTQ
                     TQARKMTGKHEVLVFATELRKGDIVLCKAGDIIPSDGEVIEGIASVDESAITGESAPV
                     IRESGGDRSAVTGGTKVISDQIVIRITSDPGSTFIDRMIALVEGAKRQKTPNEIALSI
                     LLSGLSIIFLLAVTTLPAFFAYSL"
BASE COUNT         1103 a          983 c          919 g         1278 t
ORIGIN      
        1 gcatcagttg ccccaaaatc tatcacttta tcggtcaaag agcggatacc gccacctgaa
       61 ccaattccgc cgtaagtgag ttgggttcca gttgactgag tatattggga aaagaccata
      121 ttatagtatg gcatcgggaa agtggctcct gctgcagtga gcgtgatttt tgatgcaggc
      181 tttcgtgact cgtttttgct tgacttgttt ccgcacgaat agagtacaag cacagccatc
      241 atgataatta ccaattgttt catttttcta atttttaaat tcaaaaatta ttacacttaa
      301 tatcttattt ctacattaat aaacgcgtag gttgttgctt tttgagcttc gtcggccgga
      361 ttccaatacc tgatgttcgg agaaaccctg atgcctttca ccgggctgaa ttcaagtccg
      421 cccatcagta actgaccgtc tttggcaagt tgccacggag tggtttgtcc tttcagcggt
      481 ttcgattgta aatcatcaaa acgggcgaag tatttaattt tttcgccggg tttccaggtt
      541 gtgtaaaccg atgttccgta catgtcctga ccttcgatca ttttggtatt ccactggtag
      601 ttatattcgg cagcagctac aaatttttta cctgcataag cggcaaaacc tgcaaaagag
      661 gtttgtttaa cttctttacc catgtaatct gcataaatac gtgctgtaaa acttccaagg
      721 tcttttacgg taattcctaa cgccgggcgc agaaattcgt cgcgctgatg cgatttgtaa
      781 ccttcgccgt taaaaacaga aagatcggca ctgatatttt ttgtaaaagt atagttagcg
      841 gttaccccca aatcggcact taagtataaa aagtaagcgt ccataaacac tttttcaata
      901 taacgcaagc cccagatatc ttcggagtat ttaaactgtg tggttggaat catcccgaat
      961 agtgctttaa agttttcgtt tttatactgc atatacgcaa atttcaggaa tgcggtgtat
     1021 tgcagattgc caactttggg gtcgcccacg tcaacggtta aacgtccata ccagttctgg
     1081 ctgaaattat attcgtaacc caggtaggca cgtgtaattt caaaagccgg tgtggattcg
     1141 ccatccaaaa atgcagtatt gaagttggtg aaaatcaaag ctgttggttt tcctcccggt
     1201 ttaaatgttt cggtggtggt ttgcccccag gtattcagtg ctgctaaaat tgccagcaaa
     1261 agaagtgata atgatttttt catttatttc aatatgaata attttatgct gcaaaaatga
     1321 aatatgtaca ttacaaccct gttactaaaa tattacaaaa cgattaaatt ttacggattg
     1381 tactgtgtaa agaatttttt aacaggaagt tataccggtt tcatttttgt tacaaaactg
     1441 taaaccagtg ctgaataggt cttttcgttt tcagaatatc cctcgctccc acgcgattgc
     1501 cccttaaatc cgcatgtcga gattccattt tcttcatccg ccccctcgtc tggcctgaag
     1561 atgcgaggct ataacacctg gattttaaaa attggggggc accctccaac aggtggcgcc
     1621 gtattctttc gtctttataa aacctttacg tccttcccca aaatccttat aaaaccctta
     1681 taagtttcac tgcaactttg tattgaatta aagtgacagt gtatgacaac tacaatttta
     1741 atggcagtac agcaaacgga taattttttg ggttacattt ttggagcaac cattgccctg
     1801 tttttactgg gttatctttt ctattcgctg gttaaaccgg aaaaattttg agccatgaca
     1861 acacaagatg caatcctgct gattttgttt atttccattt tagccggtct aactccggtg
     1921 gtaggaaact acctgtttat ggtttttaca gatggcaaac attttatgcg acctgttttg
     1981 ggttggctcg aaagcgtgac ttacaagttt gccggaataa accccaaaga agaaaccaat
     2041 tggaaaacat acacctcaaa tctattgcta ttcaatctga ttggtattgt tttcctcttt
     2101 gtaatccagc tcacccaggc ctatttacca ttaaatccag cccatctggg aaatacctcc
     2161 tggcatttag cctttaacac ggctgtgagt tttgtgacca acaccaactg gcaaagttat
     2221 gccggcgaaa cgacacaaag ttatttggtg caaatgcttg gatttactgt tcagaatttt
     2281 gtaagcgcag ccacggggat ggctgttctg ctggctgtca tcagaggaat ctcgcgcaaa
     2341 acaactgata aaataggaaa cttctggaca gacctgacac gcacaaccat ttacatcctc
     2401 ctgcctgctt ctgttgtact ggccattgtg ctggccggac agggagttat tcagaatttc
     2461 aaaacttacg aaacagttca aacggttgaa ggctcgctgc aaatacttcc catgggacct
     2521 gccgcctccc aaatagcaat aaaactcctg gggaccaacg gcggcgggtt ctttaatgcc
     2581 aacagcgctc atcctttcga aaacccgact ccgctaagca actttttaca aatgttggcc
     2641 atttttatta tcccgatggg attgacttac acctatggaa aaatggtcgg ttccctacgt
     2701 cacgggcgaa ccatctttct gacgatggga atcctgtttc ttctcgggct ggggatatcc
     2761 ctctattccg aatattcttc caatccgatt tttggaaatc tgccattaat ggaaggtaaa
     2821 gaaacccggt ttggaattac caacagtgtt ttattctctg tagtaaccac caatgcttcc
     2881 tgcggcgctg taaatgccat gcacgacagt ctttcaccca tttccggcat gattgccctc
     2941 atcaacatga tgctcggcga agtgattttt gggggtgtgg gcgccggact gtatggcatg
     3001 attatcttca tcatccttac cgtatttatt gccggcctta tggtgggccg gacacccgaa
     3061 tatttaggaa aaaagattga agcctttgag gtcaagatgg ccattgtcgc cgttctggcg
     3121 ccaagcctgg tgatactgct tttttctgcc tgggcatcgg taagtcctgt tggtttagcc
     3181 gggcttaaca atcccggtcc gcatggactt tcggaaatat tgtacgccta cagttcggca
     3241 gccggaaaca atggtagcgc tttcgccgga ttaaatgcca atacaatatt ttacaacctt
     3301 accactggaa ttgggatgtt gattggccgg ttcggcatca tcgttccggt tcttgcgatc
     3361 gccggcaata tggcacagaa gagagtaacc cccttgtcgt cgggaacttt ccgcaccgat
     3421 aactggctgt ttgccagcct gctcactgca gttattctca ttgttggcgg ccttaccttt
     3481 ttcccggccc tctcgcttgg cccggtggtt gaacatttac tgatgaacag cggattaacc
     3541 ttctgatttt acaaccatga gcacaaaacc tgaaataaag cttttcaaca aaaggttgtt
     3601 gcgacaggca gtccaagaga gctttgtcaa gctgaacccg gttcttttga taaaaaatcc
     3661 tgttattttc attgttgcca tcggatcggt tcttacaacc ctggttgttt ttatggacat
     3721 tttcaatggc agtttttcct ttttcaactt ccaaatagct gtttggcttt ggtttacggt
     3781 gctgtttgcc aatttttcag aagctgttgc cgaaggacgg ggaaaagcgc aggccgacaa
     3841 cctgcggaaa aatcgcaccc aaacgcaggc ccgtaaaatg acagggaagc acgaagtttt
     3901 ggtttttgcc actgaactcc ggaaaggaga tattgtcctc tgcaaagcag gcgacatcat
     3961 cccttctgac ggcgaagtga ttgaaggtat tgccagcgtt gatgaatcgg ccataacagg
     4021 cgaatcagcc cctgtgatcc gcgaaagcgg aggcgaccgc tcagccgtta ccggtggcac
     4081 gaaagttatc agcgaccaga tcgtcatccg gatcacctca gaccccggca gtacatttat
     4141 cgaccgcatg atcgccctgg tagaaggggc caaacgccag aaaaccccca atgaaattgc
     4201 gctgtcgatc ctgctttcgg gcttgtcaat cattttcctg ttggccgtaa cgacccttcc
     4261 ggcatttttc gcatatagtt tgt
//
LOCUS       DAOSDR010000272         4281 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrimonas sp. isolate AMR_MDS_1826
            NODE_7262_length_4281_cov_3.329153, whole genome shotgun sequence.
ACCESSION   DAOSDR010000272 DAOSDR010000000
VERSION     DAOSDR010000272.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863355
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrimonas sp. (wastewater metagenome)
  ORGANISM  Petrimonas sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Dysgonomonadaceae; Petrimonas.
REFERENCE   1  (bases 1 to 4281)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4281)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:10:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,720
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 35
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4281
                     /organism="Petrimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7262_length_4281_cov_3.329153"
                     /isolate="AMR_MDS_1826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2023866"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..228
                     /locus_tag="PLI30_10270"
     CDS             <1..228
                     /locus_tag="PLI30_10270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI79977.1"
                     /translation="DMWEQFYDSAATNFQSALKYMQKNNLLDDFKLRCVDCVKYASPC
                     GYGFADEIAEIFYEYYGEGAKFTNKYGDKYE"
     gene            221..838
                     /locus_tag="PLI30_10275"
     CDS             221..838
                     /locus_tag="PLI30_10275"
                     /inference="COORDINATES: protein motif:HMM:NF015629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0158 family protein"
                     /protein_id="HOI79978.1"
                     /translation="MSNTLDQILAIIRSVMDDKQKLNRILEFLEEEILIEEEFKEIEI
                     PEKFKPCITEIAGSIDAGLVCFFNPETLAIDAYPRDLLHEVDLFGDEPEEVKKNLSEL
                     YGWDDVKVLDWLIFIEFTPPDSHESFRIMEAFAERLKNADKLKNKLIDALNNRKPFAN
                     FKNIIDNSDHRQDWFDFKVRWLENSVAAQLMEELDNFQCEAFEKI"
     gene            complement(870..2576)
                     /locus_tag="PLI30_10280"
     CDS             complement(870..2576)
                     /locus_tag="PLI30_10280"
                     /inference="COORDINATES: protein motif:HMM:NF017416.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HOI79979.1"
                     /translation="MFKKSDENPQLGIFSSPTEYFRDSKKKEYLKNDSWHNRFRNHVV
                     MRVDESIFRPLYSNGTGAPNASIRILVGMMILKEGQGWSDAQLFENCAYNLLVRSALG
                     LMSLEDAEPVSSTYYLFRRNLVDYAREHGEDLFKKCQGQITRDQLLEFEVSGKQVRMD
                     SKLVGSNIAWYSRYELIHETLRLFINEREEHIFNKSLSKEMFSLIESIQGETGNKVVY
                     RSTKAEVDARFVELGKLMYRFIGLFKKHDYGHYQTLKAVFEQQYSVGRDKTVVPLEKE
                     KVSAKSIQSPHDTDCHYRDKDGNKVKGYSVNVTETCDQPGDDKDTALNLITDTEVTVA
                     SAPDNAFLEQALDRTQEIISDKIEKVYADGAYHSADNQEYCQNDKNGIELILTGMQGP
                     APRYDIRLDEQNEINLIVTDNKTGTTIQAQRVKLRKDKTQRKWRIKTEEGKYRYFDED
                     NLRAAALRKKLDDIPIDEKNIRNNVEASIFQLGYHYPNDKSRYRSLAKHKLWAYSRSL
                     WINFVRIVNYMTRISQRALFWQKLPQYIINLCFNMYIIVNILSINKNNHICPVKLHFS
                     AI"
     gene            2757..>4281
                     /locus_tag="PLI30_10285"
     CDS             2757..>4281
                     /locus_tag="PLI30_10285"
                     /inference="COORDINATES: protein motif:HMM:NF016330.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SWIM zinc finger family protein"
                     /protein_id="HOI79980.1"
                     /translation="MTKAKKTLPPYEHLFSAQSLKRAYEYVDYVENVQFSQNGISCKV
                     EGSYPYKVKIAWNDNDYTELSCTCPYDYEGYCKHIAAVLMYIEEEEEFLEIPTATKKQ
                     KKPEWRQIIEKLSKEELQAFLIEYATDDESLINTIMIKYATPIDRVDIQKYKKIVNNI
                     FSSASDDFGFIHYQDVYGVSHLISNLSDKAISTRKKAKFEESFSISAAMCEVCIDYIQ
                     NLDDSSGWIGGLIYDTFRNIENVFHECKNETLENLVFEWLSKQMKNPDYSDYGCDEGL
                     ESIYFELGSNSPYTESTSRFIDEQLSQYEKGTDWSSKYRYNKFLMHKLALLEKQEKTE
                     EAQLLIEENLQLSDIRKIKVNQLLEQKDFFKAIACIKEGIGISEQENLAGVTRNWNNL
                     LLQIYQEQNMQYELHYLARSMFLNHSESMEYYRIYKRTVEKAKWSAELASIIDELKKR
                     RKTNAWHYHFSYDLANIYIEEKMWGELFIEVKDANDISVTSRYAKYLQDGFSPQLIDI
                     "
BASE COUNT         1310 a          843 c          881 g         1247 t
ORIGIN      
        1 gatatgtggg aacagtttta tgacagcgcc gcaacaaatt ttcaatcggc attaaaatat
       61 atgcagaaaa acaatttatt ggatgacttc aaattgcgtt gcgttgattg cgtaaaatat
      121 gcttcgccct gcggatatgg ttttgccgat gaaattgctg aaatatttta tgaatattac
      181 ggtgaaggtg ccaagtttac aaacaaatac ggagataaat atgagtaaca cattagacca
      241 aatactcgcc atcatccgtt cggtaatgga tgataaacag aagctcaacc ggattttgga
      301 gtttctggaa gaggaaattc tcattgagga ggaatttaaa gaaattgaga ttcctgaaaa
      361 atttaaaccc tgcattaccg agatagccgg cagtattgat gccggccttg tatgtttttt
      421 caatcccgaa acgcttgcga tagacgctta tcctcgcgat cttttgcatg aagttgattt
      481 gtttggtgac gaaccggagg aggtgaaaaa gaacttgtcg gaattatacg gttgggacga
      541 cgtaaaagtg cttgattggc taatctttat cgaatttact ccgcccgact ctcacgaaag
      601 cttccgtatt atggaagcat ttgctgaaag actgaaaaat gcagacaaac tgaaaaataa
      661 actcattgat gcgctgaata atcgcaaacc gtttgccaat ttcaagaata tcatcgacaa
      721 cagcgaccat cggcaagatt ggttcgattt caaggttcga tggttagaga atagtgtggc
      781 cgcacaattg atggaagagt tggataattt ccaatgtgag gcttttgaaa aaatataaag
      841 tatgagtctg ctctgaaaac cccatttttt caaattgctg aaaaatgaag tttaaccgga
      901 caaatgtgat tgtttttatt aattgaaagt atgtttacaa taatatacat attgaaacat
      961 aaattaatta tatattgtgg cagtttttgc caaaaaagcg ctctttgact tattcgcgtc
     1021 atataattca ctatcctgac gaagtttatc cacaacgagc gggagtatgc ccatagtttg
     1081 tgcttggcaa gtgacctgta acggctcttg tcattcgggt aatggtatcc taactggaaa
     1141 atgcttgctt cgacattgtt cctgatattt ttttcatcta tcgggatatc gtccaatttc
     1201 ttcctcagag cggcagccct gaggttatcc tcatcgaagt agcggtattt tccttcttca
     1261 gtcttgatcc tccatttcct ctgtgttttg tctttgcgga gttttacccg ttgtgcctgt
     1321 attgttgttc ctgttttgtt gtcggttact attaaattga tttcattctg ttcgtccaaa
     1381 cggatgtcat atctcggtgc ggggccttgc atgcctgtga gtatcagctc gatgccattt
     1441 ttatcgtttt ggcaatactc ctgattatca gcactgtggt aagccccgtc ggcatatact
     1501 ttctctattt tgtcggatat tatttcctgt gtccgatcca gggcttgttc aaggaaggcg
     1561 ttgtccggag ccgatgccac cgttacctcg gtatcggtta tcaggttcaa tgccgtatct
     1621 ttgtcatctc ccggttggtc gcatgtctcg gtgacgttca ccgaataccc tttgaccttg
     1681 ttgccgtctt tgtcacggta atggcagtcg gtgtcatggg gtgactgtat ggatttggcg
     1741 ctgactttct ccttttccag aggaacgaca gttttgtccc tgccaaccga gtattgttgt
     1801 tcgaaaaccg ctttaagggt ctggtaatga ccatagtcat gcttcttgaa gagccctatg
     1861 aaacggtaca tcagtttgcc cagttcaacg aacctggcat caacctcggc ctttgtactg
     1921 cggtacacca ccttgttgcc cgtttcaccc tgtatgcttt cgataaggga aaacatctct
     1981 ttggacaggc tcttattgaa gatatgttcc tccctttcat tgataaacag acgcagggtt
     2041 tcatgaatca actcgtaacg ggagtaccag gcgatattgc tgccgaccag tttactgtcc
     2101 atgcgcacct gcttgccgct cacctcaaac tccaatagct ggtcacgggt gatttgcccc
     2161 tggcactttt tgaacaggtc ttcgccgtgt tcccttgcat agtcaaccag attgcgacgg
     2221 aaaaggtagt aagtggatga aacaggctca gcgtcctcta acgacatcag acccagagca
     2281 ctgcgaacaa gtaaattgta tgcgcagttc tcaaacaatt gtgcgtcgct ccatccctgg
     2341 ccttccttga ggatcatcat accgaccaaa atacggatag aggcattagg agcccccgtt
     2401 ccattagaat aaagtggtct gaaaatagac tcatccacac gcattacaac atggtttcgg
     2461 aaccggttat gccaggagtc attttttagg tattctttct tcttagagtc tctgaagtac
     2521 tccgtcggtg atgaaaaaat gcccaattga gggttttcgt ccgatttttt gaacatatta
     2581 tacagtttta agccctacta aggtagtgaa taatagctat atacgcaaat atttcaaaga
     2641 acttttcagg tgaaatttac ctgatttata aaatttattg tacaaaaaat tttaaactaa
     2701 attatcactc cgacttttcg gagcggactc aagtattaaa aaaaacaata cgggcaatga
     2761 ctaaagcaaa aaaaacgctt cccccctacg aacatctctt ttctgctcaa tcattaaaaa
     2821 gagcttacga atatgtggac tatgttgaaa atgtgcagtt ttcccaaaac ggtatttcct
     2881 gtaaagtgga aggtagttat ccatacaaag taaaaattgc ctggaacgat aacgattaca
     2941 cagaattatc ttgcacttgc ccatacgatt acgaaggtta ttgtaaacac attgccgccg
     3001 tgttaatgta tatagaggaa gaggaggaat ttctcgaaat accaaccgca acaaaaaaac
     3061 aaaaaaagcc ggagtggaga caaattattg aaaaattatc gaaagaagag ctacaagctt
     3121 tcctgatcga atatgctacc gatgatgaaa gcctgataaa cacgataatg ataaaatacg
     3181 caacgccaat tgacagggtg gatattcaaa aatacaagaa aattgtaaac aacattttca
     3241 gttctgcttc cgatgatttc ggatttatcc actaccaaga cgtatatggt gtttctcatt
     3301 taatatccaa tttatcagac aaagcgattt ctactagaaa aaaagccaaa tttgaggaat
     3361 cgttcagcat tagcgcagcc atgtgcgaag tatgcatcga ttatattcaa aacctggacg
     3421 actcaagcgg atggattggg ggacttattt atgacacgtt ccggaatatt gaaaatgttt
     3481 ttcacgagtg taaaaacgaa acactcgaaa acttggtttt cgaatggctt agcaaacaaa
     3541 tgaaaaatcc ggattacagc gattacggct gcgatgaagg attagaatcc atttatttcg
     3601 agctggggag taattcacca tacacagaat cgacatctcg gtttatcgat gaacagttat
     3661 cgcaatatga aaagggaacc gactggagtt ctaaatacag gtataacaag tttctaatgc
     3721 acaaattagc attacttgaa aaacaagaaa aaacagaaga agctcaatta ttgatcgaag
     3781 agaatttgca actgtccgat atccggaaaa taaaggtaaa tcagttactc gaacaaaaag
     3841 acttctttaa agcaattgcg tgtatcaaag aaggaattgg aatatcggag caagagaatc
     3901 ttgccggcgt tacccgaaac tggaataatc tgctgcttca aatctatcaa gaacaaaaca
     3961 tgcaatacga gctccattat ttggcaagaa gcatgttttt aaatcattca gaatctatgg
     4021 aatattaccg tatatataaa cgaaccgtag aaaaagcaaa atggagcgca gagttggcat
     4081 cgataatcga tgaactgaaa aaacggagaa aaacaaacgc atggcattac cacttctcgt
     4141 atgatttagc caatatttac atcgaagaga aaatgtgggg agaacttttt atcgaagtaa
     4201 aagatgcaaa cgatatctct gtaacaagta gatatgccaa atatcttcaa gacggctttt
     4261 ctccgcaact tattgatatt t
//
LOCUS       DAOSDR010000273         4271 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrimonas sp. isolate AMR_MDS_1826
            NODE_7288_length_4271_cov_3.714184, whole genome shotgun sequence.
ACCESSION   DAOSDR010000273 DAOSDR010000000
VERSION     DAOSDR010000273.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863355
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrimonas sp. (wastewater metagenome)
  ORGANISM  Petrimonas sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Dysgonomonadaceae; Petrimonas.
REFERENCE   1  (bases 1 to 4271)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4271)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:10:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,720
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 35
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4271
                     /organism="Petrimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7288_length_4271_cov_3.714184"
                     /isolate="AMR_MDS_1826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2023866"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            334..2607
                     /locus_tag="PLI30_10290"
     CDS             334..2607
                     /locus_tag="PLI30_10290"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOI79981.1"
                     /translation="MCWTKIQKEHLPYVTVQLKGTTLGTTTDATGHYFLKNLPTGNFT
                     LEVKMIGYKTITKEVKIIANTTQEIDFEMEEESIPLNAVVVSANRNETNRMLAPSLVS
                     VVDIKMLEATNSKSLAQALNFQPGLRVEDNCQNCGFSQVRINGLEGAYSQILIDSRPI
                     YGALTGVYGLEKIPSNMIDRIEVIRGGGSALFGSSAIGGVINVITKEPSRNSGDIVHM
                     FSSINGKAAENNTMFNASILTDNRKAGIMLYGQNRDRQGYDHDGDSFTEMPMLKNRSF
                     GFRSFLKTGLYSKLSIEYHNMHEFRRGGDSLKLQPFQSKITEQLEHYINGGGINYVQN
                     SADMKNKFNLYASAQHTLRKSYYGGGDPVEVIPGSNQNQEVIDNLNNRLGSFGRSTEL
                     IHHLGGQYTRSMDHLLFMPAELTAGAEYLNNTLTDVSGYRTHDVDQKTHTASGFLQNE
                     WKDDRWSILLGGRLDKHSLVKSAIFSPRANIRYNPNGKVNFRLSYSEGFRAPQYFDED
                     LHVDIAGGEHIVRVLSKDLQEERSRSVSGSIDFYHTTDNLQLNALAEGFFTHLKNKIT
                     AIDDPEVEGQKLVVNAKGENAKVYGINLEGRMAYGRLLDLQLGVTFQKSLFDQAQEPI
                     EGVGGYREFMRTPDTYGYFVTTWKPATRFSATLSGNYTGRMYVPHVKGEGDPAKDRFA
                     SENKIERVNSFFELHTKLSYDIPVLEYSTLQLNAGVQNIFNAYQKDFDTGAGRASDYI
                     YGPSAPRSVFAGIKLTL"
     gene            complement(2669..3721)
                     /locus_tag="PLI30_10295"
     CDS             complement(2669..3721)
                     /locus_tag="PLI30_10295"
                     /inference="COORDINATES: protein motif:HMM:NF025420.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4249 domain-containing protein"
                     /protein_id="HOI79982.1"
                     /translation="MNKKSIFCIILLCALLFCACEKEIEFKGDVKKPVLVLNGYLTPD
                     ENVTVHLSKSRFVMDDHTPYVSIENASVELYVNGVLKEKLVYGNEGNYAGQYRPRIGD
                     EIEVRASAPGFEAVKAKTGIPQAPVFELADSTLTHTVKDVSSQFTDDPGYTFKQWEHV
                     NKIRLRLKEEKNEENFYFIKAEHNLYDKDKSIGKELQKVELSKVLKGNISDTGNPLEE
                     VLWGEEDSGYKRQLENIFSDALVNGRELFFNFEYYENLKTVMKRPNGNEEIINAGGPF
                     TKEYVIALSSMSSDYYQFVVSAAKAYNVDENPFVEPVQVRTNVENGLGILGAYTTSRF
                     VFRFEKYTEGGYSIQW"
     gene            complement(3754..>4271)
                     /locus_tag="PLI30_10300"
     CDS             complement(3754..>4271)
                     /locus_tag="PLI30_10300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019539186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOI79983.1"
                     /translation="WSKADRIFDRPGHEINYGKVFPAKYDRRHDVSLTLMHKFSDRFD
                     ISGSWVYSTGNAATFGYQSYRTVPAPNYQESHYALQSVTYVESRNNFRYNNYHRMDLG
                     VNFHKTTRRGWKRTWNISVYNVYNQLNPFFVYPSSKTEQTGPSESRSKTVLKQVSIFP
                     VLPSVTYVVRF"
BASE COUNT         1191 a         1058 c          962 g         1060 t
ORIGIN      
        1 ctcgttcatt aactatacta tactttacga aggattacat cctgttaaaa atcaaacccc
       61 gactctcctc agagctcctc ccgcagcttg attttctcct gtttttaaga aacagtctct
      121 gaatcgtgtc cgttttcaca acggatagga tctttttatg cgtggcgaca agtgggcgac
      181 cacggagatt ttcaagttac aataaaacca actagataaa tagcattctt atgaaaaaaa
      241 gatacatact ccttctatgc ctatccactc tttttatatg gagacaggca aagggaaacg
      301 aaccctataa aactgacgca aacatcttcg gacatgtgtt ggacaaaaat acaaaaagag
      361 cacttgccgt acgttactgt acagctgaaa ggcaccaccc ttggaacaac aaccgatgca
      421 acgggacact acttcctgaa aaacctgcca acgggcaatt tcacactcga agtaaaaatg
      481 atcgggtata aaaccatcac gaaagaggtg aagatcatag ccaacaccac ccaggagatc
      541 gatttcgaga tggaagaaga aagcatcccg ctcaacgccg tggttgtgtc ggccaaccgg
      601 aacgaaacca accggatgtt ggccccctcg ttggtgagcg tggtagatat aaagatgctg
      661 gaagccacca actcgaaaag cctggcacaa gcattgaatt tccagcccgg cttacgcgtg
      721 gaagacaatt gtcagaactg tggtttctca caggtgagga tcaatggcct tgagggggct
      781 tactcgcaga tactcattga ctcccgcccc atttatggtg cattgaccgg tgtttacggg
      841 ctggaaaaga ttccctccaa catgatcgac cggattgaag taatccgcgg gggagggtcc
      901 gcactgttcg gttcgtcagc catcggcggt gttattaacg tgataacgaa agaaccttcc
      961 cgcaacagcg gggatattgt ccacatgttc tcgtccatca acgggaaagc tgcggaaaac
     1021 aatacaatgt tcaacgcatc gatccttacc gacaaccgga aagcgggaat catgctctac
     1081 gggcaaaacc gcgaccgcca aggttatgat cacgacggtg acagttttac ggagatgccc
     1141 atgctcaaaa accggtcgtt cggcttccgg tcgttcctga aaacagggct atactccaaa
     1201 ctttccatag agtaccacaa catgcatgag ttccgccgcg gaggcgacag cctcaagctg
     1261 caaccgttcc agtcaaaaat taccgaacaa ctggaacact atatcaacgg aggaggcatc
     1321 aattatgtgc aaaattcggc agatatgaaa aacaagttca acctctacgc ttcggctcag
     1381 cacacacttc gcaaaagcta ttatggcggg ggcgacccgg tggaagtaat acccgggtca
     1441 aaccaaaacc aggaagtgat cgacaacctg aacaaccggt taggctcgtt cgggcgttcc
     1501 accgaattga tccatcatct gggtggacaa tatacccgaa gcatggacca tcttttgttt
     1561 atgcctgccg agctgactgc cggagccgag tacctgaaca atacactgac cgacgtaagc
     1621 ggataccgca cccacgatgt ggatcagaaa acccataccg cgagcggttt tctacaaaat
     1681 gaatggaaag acgacaggtg gagcattctt ctgggcggcc gcctcgacaa gcactcgctg
     1741 gtaaaatcgg ctattttcag tcccagggca aacatccgtt ataacccaaa cgggaaagtc
     1801 aacttccgcc tgtcgtatag cgaagggttc cgcgcgccgc agtattttga tgaagatcta
     1861 cacgtagaca ttgccggcgg agaacacatc gttcgtgtcc tgtcgaaaga cctgcaagaa
     1921 gagcgttcac gcagcgtgag tggctcgatt gacttttacc acaccacgga caacctccag
     1981 ttaaacgcac tggcggaagg ttttttcacg cacttgaaaa ataagattac tgccattgac
     2041 gacccggagg tggaaggaca aaaactggtg gtaaatgcca aaggtgaaaa tgcgaaagta
     2101 tacggcataa acctggaagg aagaatggca tacggccgcc tgttggatct gcaactgggg
     2161 gtaacgtttc agaaaagttt gtttgaccag gcccaggaac cgatagaagg tgtaggcggg
     2221 tatcgcgagt tcatgcgcac tcccgatact tacgggtatt tcgtcaccac gtggaaaccg
     2281 gcgacccgct tttcagcaac gctttcggga aactataccg ggcggatgta cgtgccccac
     2341 gtgaaagggg aaggcgaccc tgccaaagac cggtttgcat cggaaaataa aattgaaagg
     2401 gtaaattctt tttttgagtt gcatacaaaa ttgtcttacg acattcccgt cctcgagtat
     2461 tccacgctgc aattgaacgc gggagtgcag aacattttca atgcctatca aaaagatttc
     2521 gataccggtg caggaagggc atccgattat atctacggcc catcagctcc gcgaagcgtg
     2581 tttgcaggga taaaactaac gctttgaaac atattgaatt ctgaactcca ggaaattaca
     2641 actcctggag tttaggtttc gatcaaacct accactgtat tgaatagcct ccctccgtgt
     2701 atttttcgaa tctaaaaaca aaccggcttg tggtatatgc acctaaaatc cccaatccgt
     2761 tttccacgtt tgtccgtact tgtactggct ccacaaaagg gttttcatcc acattatacg
     2821 ctttggcggc agaaaccaca aattgataat aatcggaaga catactggat aaggcgataa
     2881 catattcttt cgtgaaagga ccaccggcat tgatgatttc ttcgtttcca tttggccttt
     2941 tcatgacggt tttgaggttc tcatagtatt caaaattaaa aaacagctct cttccgttca
     3001 ccaatgcatc ggaaaaaata ttctccagtt gccttttata accggaatct tcttctcccc
     3061 acaaaacttc ttcgagcggg ttacccgtat ccgaaatgtt tcctttcaac actttgctca
     3121 actctacctt ttgcaactct tttccgatgg acttatcctt atcgtacaaa ttgtgttcgg
     3181 ctttgataaa ataaaaattc tcctcgttct tttcctcttt taagcgtaag cggatcttgt
     3241 taacatgctc ccattgctta aatgtatacc ccggatcatc ggtgaattgg ctggaaacat
     3301 cttttaccgt atgcgtaagg gtagaatccg ccaattcaaa caccggagcc tgcggaatcc
     3361 cggttttcgc ttttaccgct tcgaaacccg gagccgatgc gcggacttca atctcatcgc
     3421 caattcgcgg acgatattgc ccggcgtaat tcccctcatt gccgtaaaca agcttttcct
     3481 tcaacacccc gttcacgtat agctctacgg aagcgttttc gatagacacg tagggcgtat
     3541 ggtcatccat gacaaaacgg ctttttgaaa gatgtaccgt tacattttca tcgggcgtga
     3601 gataaccgtt caataccaga accggttttt ttacatctcc tttaaattcg atttcttttt
     3661 cgcaggcgca gaacaggagt gcgcagagca aaataataca gaatatggat tttttattca
     3721 ttgttgattg ttgtttcatt gaccgttaac tgttcaaaac cttaccacat acgtcaccga
     3781 cggcaatacc ggaaagatgg acacctgctt gagcaccgtt ttcgaacggc tctcgcttgg
     3841 ccccgtctgt tccgtttttg acgaaggata cacgaaaaac ggattcagct gattatacac
     3901 gttatatacg ctgatgttcc acgtacgctt ccacccgcgt cgggtagttt tgtgaaagtt
     3961 tacgcccaaa tccatgcggt gatagttatt gtaccgaaag ttgttgcggc tctccacgta
     4021 agtgacagat tgcaaggcgt aatgcgattc ctgataattg ggtgcgggca cagtgcggta
     4081 ggactgatag ccgaacgtgg cagcgttacc cgtgctataa acccaagatc ccgaaatatc
     4141 aaaccggtca gagaacttat gcatgagggt aaggctcaca tcgtgcctgc ggtcatactt
     4201 ggcaggaaaa acctttccgt agtttatttc atgtccgggc cgatcaaaaa tacgatcggc
     4261 tttcgaccac g
//
LOCUS       DAOSDR010000274         4258 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrimonas sp. isolate AMR_MDS_1826
            NODE_7323_length_4258_cov_3.326671, whole genome shotgun sequence.
ACCESSION   DAOSDR010000274 DAOSDR010000000
VERSION     DAOSDR010000274.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863355
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrimonas sp. (wastewater metagenome)
  ORGANISM  Petrimonas sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Dysgonomonadaceae; Petrimonas.
REFERENCE   1  (bases 1 to 4258)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4258)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:10:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,720
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 35
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4258
                     /organism="Petrimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7323_length_4258_cov_3.326671"
                     /isolate="AMR_MDS_1826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2023866"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..505)
                     /locus_tag="PLI30_10305"
     CDS             complement(<1..505)
                     /locus_tag="PLI30_10305"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019539395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOI79984.1"
                     /translation="MNVLIISTSDIHGGAAIAAFRLMNALRAKGVRAKMLVRDKRSDN
                     EYIMQAGGKIQNNLHFYGERGQIFLQNRLSRENLFDVSIANTGISVTRLPEFKRADII
                     HLHWINQGMLSISEIGEILASGKKVVWTMHDMWPFTGICHHAGPCARFHHSCGNCPYL
                     KAPLENDL"
     gene            complement(502..1662)
                     /gene="wecB"
                     /locus_tag="PLI30_10310"
     CDS             complement(502..1662)
                     /gene="wecB"
                     /locus_tag="PLI30_10310"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005802409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HOI79985.1"
                     /translation="MRKKIMLVFGTRPEAIKMAPLIREFQKYPDKFHVLVCVTGQHRQ
                     MLDQVLRLFEIRPDYDLNVMKSNQDLYDITSSILTGMRDVLKQSQPDWVIVHGDTATS
                     TVTALAAFYQKIPVAHVEAGLRTHNMYSPWPEEINRQITSRIATYNFAPTPLSKQNLL
                     DEGIKENSISVTGNTVIDALYWVTQKIKTDKNLRKEIEETLIRSGLPEKMGGSLTPQL
                     VLITGHRRENFGEGFTHICQAIKTLSEKFPETHFIYPMHLNPNVRETIREVFSDDKHE
                     NLHLIEPLDYLPFVYLMEKSRLILTDSGGIQEEAPGLGKPVLVMRDTTERPEALEAGT
                     VRLVGTNYSLIVSETSRLLSDDSYYYSMSTRTNPYGDGKACERIVEKMLEIA"
     gene            complement(1667..2872)
                     /gene="wecC"
                     /locus_tag="PLI30_10315"
     CDS             complement(1667..2872)
                     /gene="wecC"
                     /locus_tag="PLI30_10315"
                     /EC_number="1.1.1.336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004293527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0089714 - UDP-N-acetyl-D-mannosamine
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetyl-D-mannosamine dehydrogenase"
                     /protein_id="HOI79986.1"
                     /translation="MKACFIGLGYIGLPTAVIAADNGIEVIGVDINPEVVKSVNSGKV
                     HFSEPGLDKICKKVTSNGTLKAYTEPQTSDVYVIVVPTPFKGNHEPDVSYVESAVTTV
                     LPYLKEGDLCIIESTCPVGTTEKMAELIYSEKPALKDKIYLSYCPERVLPGNTVYELI
                     HNDRVIGGIDEGSSEKAAGFYSHFVKGSLYKTNARTAEMCKLTENASRDVQIAFANEL
                     SIICEKAHINVWELIELANKHPRVNILQPGSGVGGHCIAVDPYFITSEFPIESQLIGK
                     AREINNYKAFWCSEKILSTMKDFNLREGREPVVALMGLSFKPNIEDLRESPAKYIVSK
                     VMQGHNNADFLIVEPNIETHKVFKLTDYAEAYDTADIVAFLVAHKEFKNLIFRDDKVI
                     LDFCGVFKK"
     gene            complement(2974..4182)
                     /locus_tag="PLI30_10320"
     CDS             complement(2974..4182)
                     /locus_tag="PLI30_10320"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013546489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HOI79987.1"
                     /translation="MKVLYVFGGIPPYANALLNKLADKGVEVVVVVPPKKSTMIGKGV
                     RVIGREVANYRVITSEERKSLLGKTGFRELPSIIYREKPDIFVVGWPFFLQYFTQPEL
                     RKALKETKSKFVMREIPFQVPPYGKLSSFYKENPVYDEDMNLLNKGLFFHIRQKLVAE
                     IRKYCYSHADGAVVYSSVGKEILPTYNLSEKSVFVTYNSNNSEELLRIRQELSKEQPL
                     LPQNDHRMIHIGRLVKWKRVDLLMDAFSKVLSHFPGAELVIVGDGPEMENLKKQARDL
                     KIGSRVIFTGEIYDNLLIGRYFLASSVYVLAGMGGLSINDAMTFSLPVICSVCDGTER
                     DLVYEGSNGFFFRENDAVDLSEKIIALFNDPGLRKKMGEHSFEIIKNKVNLDTVSDRY
                     VNAYKTILSN"
BASE COUNT         1151 a         1024 c          831 g         1252 t
ORIGIN      
        1 aaagatcgtt ctccaatgga gctttcaagt atggacaatt tccgcatgaa tgatgaaagc
       61 gtgcacacgg tccggcatga tgacaaattc cggtgaaggg ccacatatcg tgcatcgtcc
      121 acaccacctt tttaccgctg gcaaggatct ctccgatttc gctgatcgat aacatcccct
      181 gattgatcca gtgtagatga ataatgtcag ctcgcttaaa ttcgggaagc cgggtaacgg
      241 aaattccggt attggcgatg gaaacatcaa aaaggttctc ccgcgataac cggttttgca
      301 aaaatatttg tccgcgctcc ccataaaaat gcaggttatt ttgaattttg ccgcctgctt
      361 gcataatata ctcattgtca gaacgtttat cacgaaccag catcttcgcc cttaccccct
      421 tggcacggag agcattcatc aaccggaaag ctgcaatggc tgccccgccg tgaatatcgg
      481 atgtgctgat tataagtacg ttcatgctat ttccaacatt ttctcaacga tacgttcaca
      541 tgccttgccg tcaccgtatg gattggttct ggttgacata gaataataat agctatcgtc
      601 cgataaaagc cgggaggttt ctgacacaat caggctgtag ttcgttccga ccagcctgac
      661 tgtcccggcc tccagtgctt cgggacgctc cgtggtatcc cgcatgacca gaaccggctt
      721 acctaagcct ggagcttctt cctgaattcc tccactgtcg gtcaggatca acctgctctt
      781 ttccatgagg tagacaaaag gcaggtaatc caggggttct attaaatgga ggttttcgtg
      841 cttatcatca ctaaacacct cacgaatggt ttcgcgcaca ttcggattaa ggtgcattgg
      901 gtaaatgaaa tgagtttcgg ggaacttttc cgataacgtt tttattgctt ggcaaatatg
      961 tgtaaatcct tcaccaaaat tttccctcct gtggccagta attaatacaa gttgcggggt
     1021 aaggcttccg cccatttttt cgggcaaccc actccggatt agggtttcct cgatctcttt
     1081 ccgaaggttt ttatcggttt ttattttttg agtaacccaa tacagcgcat caataaccgt
     1141 atttcctgtt actgaaatac tgttttcttt gattccctca tccaacaggt tctgcttgct
     1201 taagggagta ggtgcaaaat tgtatgtggc tatccggctt gtgatttgac ggttaatttc
     1261 ttccggccaa gggctgtaca tgttgtgtgt tctcagccct gcttcgacat gcgctaccgg
     1321 tattttctgg taaaaagcag ccaacgcggt taccgtggaa gtggctgtgt cgccatgtac
     1381 aatcacccaa tcgggttgcg actgcttcag cacatccctc atacccgtta atatgctgga
     1441 tgttatgtcg tataagtcct gattgctttt cataacattc aagtcataat cgggtcttat
     1501 ttcaaacaga cgaagaacct gatccaacat ttgccggtgt tgcccggtta cacaaacaag
     1561 aacatgaaat ttatcgggat atttctgaaa ctccctaatc aacggagcca tttttatagc
     1621 ctcgggacgg gttccaaaaa caagcattat tttttttcgc ataccctcat tttttaaaaa
     1681 ccccacagaa atcaaggatc accttatcat ctctgaaaat caaattctta aactccttat
     1741 gagctaccag aaacgcaact atatcggccg tgtcatacgc ttccgcataa tcggtaagct
     1801 taaaaacctt atgcgtctct atattgggct caacgattaa gaaatcagcg ttgttatgcc
     1861 cttgcattac tttcgagaca atgtatttgg caggtgactc cctcaaatct tcgatgttcg
     1921 gcttaaaaga aagccccatc aaagccacaa caggttcccg gccttcgcgt aaattgaaat
     1981 ccttcatcgt ggaaagaatt ttctctgaac accaaaaagc tttgtaattg tttatttcac
     2041 gggcctttcc tatcagttga gactcaatcg gaaattctga agtgatgaaa tagggatcca
     2101 cggcaataca atgtcctccc accccacttc cgggttgaag aatgtttaca cggggatgtt
     2161 tgttggctag ctcgatcagt tcccagacat tgatatgggc tttctcacat atgatggaaa
     2221 gctcgttagc aaaagcaatc tgcacatcgc gagaagcatt ctctgtcagt ttacacattt
     2281 cggcagtacg cgcatttgtc ttgtacaggg atcctttcac aaaatggctg tagaagccgg
     2341 cagctttttc cgaggatcct tcgtcgatac ctcctatcac acggtcgttg tgaattaatt
     2401 cataaacggt atttcccggc aatactcttt caggacaata actcaaatag attttatctt
     2461 ttaacgcagg cttttcggaa taaatcaact ctgccatctt ttccgtggtg cccacagggc
     2521 atgttgactc aataatacac aaatctcctt ccttcaagta aggaagaacg gtggtgacag
     2581 cagactccac gtacgagacg tcgggttcat gatttccttt aaacggggtg ggcaccacaa
     2641 tgacgtaaac atcacttgtt tgcggctcag tatatgcttt aagggttccg ttggaagtga
     2701 cttttttgca tatcttatcc aatccgggtt ctgagaaatg aactttcccg gaattcacac
     2761 ttttcactac ttcgggatta atgtctacac cgattacttc aataccgtta tcagccgcga
     2821 ttacagctgt cggcaaacct atataaccca atccaataaa acaagctttc atatactaat
     2881 ccattaattc aactacaaaa ataagcgtaa aattcctaat aaaacggtta acgccacata
     2941 tatacccatt ttctttgacg ggagaaagac aaatcaattc gataaaatag ttttgtaggc
     3001 atttacatac ctatcgctga ccgtatccag gttgacctta tttttgatga tttcgaagga
     3061 atgttctccc attttttttc ttaagccggg atcattaaac agcgcaatta tcttttcgga
     3121 cagatctacg gcatcatttt cccggaaaaa gaatccgtta ctcccttcgt aaaccagatc
     3181 ccgctcggta ccgtcacata ccgaacatat taccggaagg gagaacgtca tggcatcgtt
     3241 gatggacaac ccccccattc ctgccaaaac ataaaccgat gacgccaaga agtatcttcc
     3301 tatcaataaa ttgtcataaa tctctccggt aaaaataact cggctgccga tcttcaagtc
     3361 acgggcttgt tttttcagat tttccatttc ggggccatct cctacgatca ccaactccgc
     3421 accggggaaa tgcgatagta cttttgaaaa ggcgtccatc aataaatcca cccttttcca
     3481 tttcaccaac cttccgatat gaatcatcct gtgatcattt tgaggcaaca gaggttgttc
     3541 tttggacagt tcttgtctga tccggagcaa ttcctcggaa ttgtttgagt tgtaggtaac
     3601 aaacaccgac ttttcggata ggttataagt aggcaagatc tctttcccta cagacgaata
     3661 gactaccgca ccatcagcgt gtgagtaaca gtatttcctg atttctgcaa cgagtttctg
     3721 ccgtatgtgg aaaaacaacc ctttattcag caggttcata tcttcatcgt atacagggtt
     3781 ttctttgtag aaggagctga gttttccata gggtggaacc tgaaaaggga tttcgcgcat
     3841 cacaaatttg gatttggtct cctttaaagc tttccttaat tcaggttgag tgaaatactg
     3901 caagaaaaaa ggccagccta cgacaaaaat atcaggcttc tctctgtata tgatggaagg
     3961 aagttccctg aagccggttt tgcccagcag cgattttctt tcttcactcg ttataaccct
     4021 gtaatttgcc acctcccggc caatcaccct tacgcctttt ccaatcattg tacttttttt
     4081 cggaggaaca accaccacaa cttccactcc tttatcagcc agcttattca acaaagcatt
     4141 ggcataaggt ggaattcctc caaaaacgta caatactttc atttacagcg gaatttaaaa
     4201 ttcaattttc gaggacaaca cattccggcg gaaaacgcct aaatttatat tcactccc
//
LOCUS       DAOSDR010000275         4254 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrimonas sp. isolate AMR_MDS_1826
            NODE_7333_length_4254_cov_4.441296, whole genome shotgun sequence.
ACCESSION   DAOSDR010000275 DAOSDR010000000
VERSION     DAOSDR010000275.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863355
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrimonas sp. (wastewater metagenome)
  ORGANISM  Petrimonas sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Dysgonomonadaceae; Petrimonas.
REFERENCE   1  (bases 1 to 4254)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4254)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:10:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,720
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 35
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4254
                     /organism="Petrimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7333_length_4254_cov_4.441296"
                     /isolate="AMR_MDS_1826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2023866"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1006)
                     /gene="gnd"
                     /locus_tag="PLI30_10325"
     CDS             complement(<1..1006)
                     /gene="gnd"
                     /locus_tag="PLI30_10325"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylating NADP(+)-dependent
                     phosphogluconate dehydrogenase"
                     /protein_id="HOI79988.1"
                     /translation="MKQKADIGLIGLAVMGENLVLNMESKGYTVAVYNRTVEKVDNFV
                     SGRGKGKNFIGAHSLEELVDSLERPRKVMMLVKAGKPVDDFIEQLIPLLEPGDIIIDG
                     GNSHFPDTVRRTKQVESKGLLYIGTGVSGGEEGALLGPSMMPGGSPEAWPFVKEIFQA
                     VAAKVEDGSPCCDWVGEDGAGHFVKMVHNGIEYGDMQIINEAYHLMKELLGMDAFEQH
                     EVFKKWNAGKLDSYLIEITTDILAYKDEDGSPLVEKILDTAGQKGTGKWTAVTALDLG
                     IPLTLIGESVFSRCLSAQKDLRVKASKVIAGKKPAFKGDRQQFINDLEQAIYGAKIIS
                     Y"
     gene            complement(1029..2105)
                     /locus_tag="PLI30_10330"
     CDS             complement(1029..2105)
                     /locus_tag="PLI30_10330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019538275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HOI79989.1"
                     /translation="MLQLKRECKYSLIVASSMGVRITPVNAQPVHSSKIFEMQATSAE
                     TNVASISSSLGLPVKVLTTFVKDSPIAAFIKSDLRARNIDFEGPEIPQGGPWGYRHQF
                     NIADSGFGLRGPRVQNDRAGEVGKTLNVKDFDLERIFEKEGVQIMHLSGLIAALSPET
                     SDFCLQLARYAKRHGTLISFDLNHRATFWKGREKELKEAFAEIASLSDILIGNEEDFQ
                     LCLGVQGPKAGGENIEDTIDAFKGMILNARKAFPNVSVFANTLREVISANEHLWGAIV
                     YENENWHVAPLRKINVVDRIGGGDGFVGGLLYSILKEMPSEKWIQFAWASGALATTFL
                     TDYAQPADEEMIWSIWKGNARVKR"
     gene            complement(2129..2941)
                     /locus_tag="PLI30_10335"
     CDS             complement(2129..2941)
                     /locus_tag="PLI30_10335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008643544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOI79990.1"
                     /translation="MYELFNIQGKVIVVTGGTGVLGASMVEYLALHGAKVAVLARNKE
                     KGDEQIEHVRSKGGDALFLQTDVTDEAVLRQNAEAIIARYGKIDALINGAGGNMPGAT
                     IGPKNTIFDLKMDDFRKVVDLNLMGTVVPTVIFAEYMVKEKKGNIINISSASALRPLS
                     RVAGYGAAKAAVTNFTKYMAGELAIKFGEDFRVNALCPGFFITEQNRNLLTNPDGTYS
                     DRGNTIIAHTPFRRFGDPTDLLGTLHYLVSDASRFVTGTVAVVDGGFDAFSI"
     gene            3159..4232
                     /locus_tag="PLI30_10340"
     CDS             3159..4232
                     /locus_tag="PLI30_10340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019540251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HOI79991.1"
                     /translation="MKKRIRIKDIAAQAGVSSGTVDRILHNRGNVSDKARIAVEKVLK
                     QVGYKPNIHLSGLSLKKTYEIVITTPHASRGEYWESIHNGIQEAIDQHESLHIKTTYL
                     TYDQYNIYSCQNVFTQILILEPDAVIIGPTFKDETIALANKLTEKNIPFTFVDSMVEG
                     TSPLAFYSANHYICGYLMCKLLKSLIPESSDIGVLQAIRIGDQSANTSILRRKGAYDY
                     LTETNFSNRIHHIQFSVYEAQRNEEKMRDFFSSHPNVKGIIVLNSRGNVIADFLEDNN
                     INDVKLVGVDLTKPNICALKNERIIFLIDQNPDYQGYMAMKTLLEYLILQTKPKVENY
                     MPLNIVTKETIDLQLEFNFLKKI"
BASE COUNT         1154 a         1051 c          832 g         1217 t
ORIGIN      
        1 catacgaaat gattttcgct ccgtaaatgg cctgttcgag gtcgttaatg aattgttgcc
       61 tgtctccttt gaatgctggt ttttttcctg caatcacctt cgatgctttt acacgaaggt
      121 ctttctgagc cgaaaggcag cgcgaaaaca cggactcgcc gataagcgta agcgggattc
      181 ccagatcgag cgccgttacg gctgtccatt tcccggtacc tttctgtccg gccgtatcga
      241 gaattttttc taccaaaggg ctcccgtctt cgtctttgta cgccaggata tcggtggtga
      301 tttcaataag atacgaatcg agttttccgg cattccattt tttaaacact tcgtgttgtt
      361 caaaagcatc catccccagt aattccttca tcaggtgata tgcctcatta attatctgca
      421 tgtccccgta ttcgataccg ttatgtacca ttttcacgaa atgtccggca ccgtcttcgc
      481 ctacccagtc gcaacacgga gatccatcct ctacttttgc cgctaccgcc tgaaaaattt
      541 cttttacgaa aggccatgct tccggcgatc cgccgggcat catcgaaggc ccgagcaatg
      601 caccttcttc acccccggaa acaccggtac ctatgtacag caatccttta ctttccacct
      661 gttttgtacg gcggacggta tccgggaaat ggctgtttcc cccatcaata atgatatctc
      721 cgggttcaag gagcggaatg agctgttcaa tgaaatcatc taccggcttt ccggctttta
      781 ccaacatcat taccttacgc gggcgttcga gggaatccac caattcctca agtgaatgtg
      841 ccccgataaa attcttgcct ttacctctgc cgctcacaaa attatctact ttttcaaccg
      901 tgcggttata aacggctact gtgtaacctt tactttccat gttcaggacg aggttttcgc
      961 ccattactgc aagcccaatg agtccaatat ctgctttttg tttcatatta tttttttgat
     1021 aggttgtttt atcgtttcac ccgcgcattt cctttccata tgctccagat catttcttca
     1081 tcggcaggct gggcataatc ggtgaggaaa gtggtggcca aagcgccact ggcccacgca
     1141 aactggatcc acttttcaga cggcatttcc ttcagaatac tatagagcaa tccacccacg
     1201 aaaccgtcac cccccccaat acgatccacc acgttaatct tgcgcaacgg ggcaacgtgc
     1261 cagttctcat tttcataaac aattgctccc caaagatgtt cattggcact gatcacctcc
     1321 cggagcgtat ttgcaaatac tgaaacgttg ggaaatgctt ttcttgcatt caaaatcatc
     1381 cctttaaagg catcaatggt gtcttcaata ttttcacccc ctgctttcgg accctggaca
     1441 cctaaacaga gttgaaaatc ttcctcgttt ccaattaaga tatccgacag ggaagcgatt
     1501 tcagcaaacg cctctttcaa ctccttttcc cggcccttcc agaatgttgc cctgtggttc
     1561 aggtcaaaag agataagggt gccgtgtctt ttggcatagc gggctagctg gaggcagaaa
     1621 tcacttgtct cgggcgataa ggcagctatc aatcccgaca aatgcatgat ctgaactccc
     1681 tctttttcaa aaatccgttc caaatcaaaa tctttcacgt tcaaggtctt tcctacttcc
     1741 cctgccctgt cgttctggac acggggccca cgtaatccaa aaccactatc ggcaatattg
     1801 aactggtgac ggtaacccca cgggcctccc tgcgggatct ccggcccttc gaagtcaatg
     1861 tttcttgccc tcaggtcgga tttgataaaa gccgcaatcg ggctgtcttt tacaaaggta
     1921 gtcagcacct tgaccggaag tccgagagaa gatgaaatac tagccacatt tgtttcggcg
     1981 ctcgttgcct gcatttcgaa aatcttgctg ctatgtaccg gctgggcatt taccggcgta
     2041 attctcactc ccatactgga agcgactatc agtgaatact tacattctct tttaagttga
     2101 agcataatgg tgaagaagtt aagtctgttt aaatactaaa ggcatcaaaa ccgccatcca
     2161 caacagccac ggtaccggta acgaatctag aggcatcgct taccagatag tgcagtgtac
     2221 caagcaggtc tgtcggatca ccgaaacgcc tgaaaggggt atgggcaatt atggtatttc
     2281 ctctatcgga ataagtcccg tcaggattag taagcaggtt tctgttctgt tcagtaatga
     2341 aaaatcccgg acaaagtgca tttacacgga aatcttcacc aaacttaata gccagttcac
     2401 cggccatgta tttggtaaag ttggtaacag ctgcttttgc tgcgccgtat ccggccacac
     2461 gtgataacgg acgcaacgcc gaagcggatg aaatattgat gatgtttccc tttttctctt
     2521 tcaccatata ttcggcgaag ataactgtcg gaacaacagt tcccatcagg ttcaggtcta
     2581 ccaccttacg gaaatcatcc attttcaaat cgaaaatagt gtttttcgga ccgatagtgg
     2641 caccgggcat attgcctcca gctccgttaa taagggcatc tattttaccg tatctggcaa
     2701 tgatcgcctc tgcgttttgc cttaatactg cctcatcagt cacatcggtt tgaaggaaca
     2761 acgcatctcc accctttgat cttacatgct caatctgctc gtcccccttt tctttgttcc
     2821 gggccaatac ggctaccttc gctccgtgca aagccaagta ctccaccatc gatgcgccca
     2881 aaacgcctgt tccgccggta acaacaatta ctttgccttg aatgttaaat aattcgtaca
     2941 tgatttattt ttgtttattg attgaattca cgcctacttg tgtacgtgca caaagttaac
     3001 acgaatagac ttacagcttt cattataaat cattaattat tcttaaatat tttataattt
     3061 tactaatgta aaaagatggt tgtgtattta cacatttgca gaaacttttt ctatatttgc
     3121 attttaattt gtaaatcgat aaatatccca agtcctcaat gaaaaaacgt atcagaatca
     3181 aagacatagc tgctcaggct ggagtatcct cgggtacggt ggacaggatc ctgcacaacc
     3241 gcggcaatgt atcggataaa gcaaggatag ctgtggagaa ggtgttgaaa caggtgggtt
     3301 ataaacccaa tatccacttg tcgggtcttt cgttgaaaaa aacgtatgag atagtgatta
     3361 ccactcctca tgcttctcgc ggtgagtact gggaaagtat tcataacggt attcaggaag
     3421 cgatcgatca gcacgaaagc ttgcatatca aaaccaccta cctgacctac gaccaataca
     3481 acatttattc ttgtcagaat gtctttaccc aaatccttat ccttgaacct gatgcggtta
     3541 tcattggtcc cacttttaaa gacgaaacta ttgccctcgc aaataaactg acagaaaaaa
     3601 atatcccgtt tacatttgtc gactccatgg tcgaagggac atcacccctg gcattttatt
     3661 cagctaacca ctacatttgc ggctacctga tgtgtaaatt gttaaaatca cttatcccgg
     3721 aatcaagtga tatcggggta cttcaagcca tccggattgg ggaccaaagt gcaaatacaa
     3781 gtatcctccg acgaaaaggc gcatacgatt atttgactga aaccaacttc tccaaccgga
     3841 tacaccatat ccagttttct gtttatgaag cccagcggaa cgaagaaaaa atgagggatt
     3901 ttttctcttc gcatcccaac gtaaaaggca taatagtgct gaactccagg ggaaatgtaa
     3961 tagcagattt tttggaagac aacaacataa acgacgtaaa gctggttgga gtggatttga
     4021 caaaacccaa tatctgtgct ttaaaaaatg aacgaattat atttctcatt gatcaaaatc
     4081 ctgattatca gggatatatg gcaatgaaaa cactgcttga atacttgatt ttgcagacca
     4141 aacccaaggt tgaaaattac atgcctttga atattgtaac caaagagacc attgacctgc
     4201 aacttgaatt taattttttg aaaaaaattt agaaacattt ctcttactcc aacg
//
LOCUS       DAOSDR010000276         4245 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrimonas sp. isolate AMR_MDS_1826
            NODE_7352_length_4245_cov_4.373747, whole genome shotgun sequence.
ACCESSION   DAOSDR010000276 DAOSDR010000000
VERSION     DAOSDR010000276.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863355
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrimonas sp. (wastewater metagenome)
  ORGANISM  Petrimonas sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Dysgonomonadaceae; Petrimonas.
REFERENCE   1  (bases 1 to 4245)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4245)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:10:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,720
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 35
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4245
                     /organism="Petrimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7352_length_4245_cov_4.373747"
                     /isolate="AMR_MDS_1826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2023866"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..754
                     /locus_tag="PLI30_10345"
     CDS             <1..754
                     /locus_tag="PLI30_10345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019538190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI79992.1"
                     /translation="GSTEINSAYSYGGDNVSDSSGELTYVKIEYTGARSSANVEHNGL
                     TLNGVGNGTKIENIYIPYGADDGIEFFGGSVNVKNLLVVDSDDDMFDFTQGYTGTLEN
                     AYGIWQKGYSSSESDPRGIEADGNLDGLNPDEKGQSDFTVKNMTIELKLDASSDVKMQ
                     MQDVIKIRRGAKTTVTNALVKGVGTAMDLVDCTDGKGDAAAGTVINLTNSLTSPITGK
                     DIKPGANAATIDIKTGNTGCPTDVFAWTGYKF"
     gene            877..1362
                     /locus_tag="PLI30_10350"
     CDS             877..1362
                     /locus_tag="PLI30_10350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007658826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin system YwqK family antitoxin"
                     /protein_id="HOI79993.1"
                     /translation="MKNKTILLLGVFLLTLSVQAQDQQGNPQIYQNEGVYYRDKKQNE
                     LYTGDYREYYDNGTLKLEMQIKNGLPEGAYLIYFDNRKPKEVRSYKEGKLHGLWRTYD
                     ISGQLISEAEYKNGAKHGTWRIWDELGNQRYEMNYYEGNKTGLWRMWDDKGNLLDEKK
                     Y"
     gene            1458..1817
                     /locus_tag="PLI30_10355"
     CDS             1458..1817
                     /locus_tag="PLI30_10355"
                     /inference="COORDINATES: protein motif:HMM:NF025092.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HOI79994.1"
                     /translation="MKTILVLIATVLLSVSVYAEDNKNDKNVKSTAVTEAVRKVNTVD
                     ISGSVVDNKSNESLAGAAIFIDGNKYYSDLDGNFSVSDLKPGKHTVRVELISYQPSEM
                     QVDLQKDKEINIGLIQE"
     gene            2082..2252
                     /locus_tag="PLI30_10360"
     CDS             2082..2252
                     /locus_tag="PLI30_10360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI79995.1"
                     /translation="MAKRTFSQTQFGFIAILWVILAGYILMNAEINAITIISLIMSGI
                     IVFVPVYRNLRK"
     gene            2371..3705
                     /locus_tag="PLI30_10365"
     CDS             2371..3705
                     /locus_tag="PLI30_10365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010993399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HOI79996.1"
                     /translation="MKYEEIISLVKRRFPNEPEYLQAVEEVIESIEEVYNQHPEFEKA
                     NLVERLIIPDKIHTFRVTWVDDKGNVQTNMGYRIQHNNAIGPYKGGIRFHASVSPSIL
                     KFLAFEQTFKNALTTLPMGGAKGGSDFSPRGKSEGEVMRFCQAFTEGLWRVIGPDTDV
                     PAGDIGVGGREVGYMFGKYKKLAGEFTGTFTGKGREFGGSLIRPEATGYGNVYFLLEM
                     LKTKNIDIKGKRCLVSGSGNVAQYTCEKLIELGAIPVTLSDSDGYIYDPDGITSEKLK
                     FVMDLKNLYRGRIREYAEEYGCKYVAGARPWGEKADIALPSATQNEIDGDDAQSLVNN
                     GIFAVSEGANMPSTPEAIEIFQKHKILYAPGKAANAGGVSVSGLEMTQNSERYSWSAE
                     QVDEKLKWIMGNIHENCVKYGTEPDGYVNYVKGANIAGFMKVAKAMMGQGIL"
     gene            3794..3867
                     /locus_tag="PLI30_10370"
     tRNA            3794..3867
                     /locus_tag="PLI30_10370"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3828..3830,aa:Arg,seq:tct)
BASE COUNT         1311 a          807 c          962 g         1165 t
ORIGIN      
        1 agggtctacc gaaattaaca gcgcctatag ttacggaggc gataacgttt cggatagctc
       61 aggtgaattg acatacgtta agatagaata taccggcgcc cgttcgagtg caaacgttga
      121 acacaacgga cttacgctga acggcgttgg aaatggcacc aagatcgaaa acatttacat
      181 cccttatggg gcagacgatg ggatagaatt ttttggcggt agcgtaaacg tgaaaaacct
      241 gttggtggtt gactcggacg atgacatgtt cgactttact caagggtaca caggaacact
      301 ggaaaacgca tacggaatct ggcaaaaagg ttactccagc tcagaatcag accctcgcgg
      361 aattgaagct gatggaaacc tggacggttt aaatcccgat gagaaagggc agtccgactt
      421 caccgtcaaa aacatgacta tcgaacttaa gctggatgcg agcagcgatg tgaaaatgca
      481 aatgcaggat gtcatcaaga tccgtcgtgg agccaagact accgttacca atgctctggt
      541 gaaaggcgtt ggcacagcta tggatttagt tgactgtaca gacggtaaag gtgatgccgc
      601 tgcgggaaca gtgataaatc ttacaaatag cttaacctct cccattaccg gcaaagacat
      661 aaaaccgggt gcaaatgccg caacgataga tattaaaacc ggaaataccg gctgcccaac
      721 cgatgtattt gcttggaccg gatacaaatt ttaatcagaa caatcggttt ataaaaacag
      781 gaggtatttc gtgtaaaaac cggagtacct ttttgttttt atgcaccagg aaattgtatc
      841 tttgctatgg aggtacaact taaaaacaag aatcgtatga aaaataaaac aatactattg
      901 ctgggtgttt ttcttttaac gttaagtgtt caggctcaag atcagcaggg taatcctcaa
      961 atctatcaga atgaaggagt ctattacaga gacaagaaac aaaacgaact ttataccgga
     1021 gattatcgtg aatattacga taacggcaca ctcaagcttg agatgcagat taaaaacggc
     1081 ttacccgaag gagcttacct gatttatttc gataaccgta aacccaaaga agtgcgttct
     1141 tacaaggaag gtaaattaca cggcctgtgg cgtacgtacg atatttcggg acagctcatt
     1201 tcggaagctg aatacaagaa tggcgcaaaa cacggcacct ggcgtatttg ggacgaattg
     1261 ggaaatcagc gttacgaaat gaattactac gaaggcaata aaaccggctt gtggcgtatg
     1321 tgggacgaca agggtaatct attggatgag aaaaaatatt aacaaaaact taaccgaaac
     1381 ttaaccgttc tgtaacattt atgcctcacc tttgtatcgg tttaaaacgg aaaattttca
     1441 ataattagat acataggatg aaaacaattt tggtattaat cgcaacggtt ttattgtctg
     1501 tttcagtgta tgctgaagac aacaaaaacg acaagaatgt aaaaagtact gctgttaccg
     1561 aagctgtacg gaaagtgaat acggtggata tatcgggttc cgttgtcgac aacaaaagta
     1621 acgaatcgtt ggctggtgca gctatcttca ttgacggtaa caaatattat tcggacctgg
     1681 acggaaactt ttcggtttcg gatcttaaac cgggtaaaca taccgtacgt gtggaattga
     1741 tttcgtatca accttctgaa atgcaagtag atttgcagaa agataaagaa atcaacattg
     1801 ggctgataca ggaataggag atcagttgag aaaacattga aaagtttgct tcatagcaaa
     1861 ctttttgttt tttatgtcag gaaaagttaa ccaaaactta actgaaatgt agcgggttcg
     1921 taacaaaaga taccgatatt tgcaacgttg aaacaatatt catttggttc aaaaatagca
     1981 aaaaatcaat ttttcattaa ctctctatgg gtctttgtaa atggtgaaga tgtgaatctg
     2041 caccattttt tattatcttt gtaaaccaca ttgttcccat tatggcaaaa cgaacatttt
     2101 cacaaacgca attcggcttc atcgccatac tttgggtgat tttagccggg tatatcctta
     2161 tgaacgcaga gataaacgct atcacgatta tttccctgat catgtcgggg attatcgtat
     2221 ttgttcctgt ttacagaaat ctacgaaaat aaactttttt ctgtcaaaac ataaaaaaat
     2281 gatgaaatat tctttcattt aaatttttga tactattttt gtatttcaaa gtgattgttt
     2341 acaaagttat cacaattaaa tccttttttt atgaagtacg aagaaattat cagtctggta
     2401 aaaagacgtt ttccaaatga gcctgaatat cttcaggctg ttgaagaagt gattgagtcg
     2461 atcgaagagg tgtacaacca acatcctgaa ttcgaaaagg cgaacctggt cgaacggttg
     2521 attattcccg acaagattca tacgtttcgt gtcacctggg tagatgacaa gggtaacgta
     2581 caaacgaaca tgggataccg tatacagcac aacaacgcta ttggccccta caaaggagga
     2641 attcgttttc acgcatccgt gtctccttcc atcttgaaat tcctggcttt tgaacaaacc
     2701 ttcaaaaacg cgcttaccac acttcccatg ggtggtgcca agggcgggtc agacttttct
     2761 ccacgcggta aatcggaagg tgaagtcatg cgcttctgcc aagcttttac cgaaggatta
     2821 tggcgggtta tcgggcccga taccgatgtc ccggccggcg atattggtgt gggcggccgc
     2881 gaagtgggct atatgtttgg gaaatacaaa aagctggccg gtgagttcac gggaacgttt
     2941 accggaaaag ggcgtgagtt cggcgggtca cttattcgtc cggaagctac tggttacgga
     3001 aacgtttatt tcctgctgga aatgctgaaa accaaaaata tcgatataaa aggaaaaaga
     3061 tgcctggtat ctggctcggg gaacgtggct caatatacgt gcgagaagct gatcgagcta
     3121 ggtgcaattc cggttaccct ttccgactcc gacggataca tttacgatcc tgacggaatt
     3181 acatcggaaa aactgaaatt tgtgatggat ttgaaaaacc tctatcgcgg ccgtatccgt
     3241 gagtatgcag aggaatacgg ttgcaagtac gttgctggtg cccgcccgtg gggtgagaaa
     3301 gccgatattg cactgccttc tgccacacag aacgaaatcg acggagacga tgcccagtca
     3361 ctcgtgaata acggaatctt tgccgtatcg gaaggtgcca atatgccgag caccccggag
     3421 gcgatcgaga ttttccagaa acacaagatc ctctatgctc ctgggaaggc agccaatgcc
     3481 ggtggagtat cggtgtcggg cctggaaatg acgcaaaatt ccgaacgtta cagctggagc
     3541 gctgagcaag tggacgaaaa gctaaaatgg attatgggca atattcacga aaactgtgta
     3601 aaatacggta ccgaaccgga tggttatgta aactacgtga aaggggctaa tattgccgga
     3661 tttatgaaag tagccaaggc gatgatgggg cagggaattt tgtaatttta tcgatttgcc
     3721 ttaaattcag tttcataaat tttttttatt ctcagaattg ccgtattttt gcagcctgaa
     3781 tttgcacaag gagggtcggg tagctcagct ggatagagca acagccttct aagctgtggg
     3841 tcctgggttc gaatcccagc ctgatcacgt tagaaacgaa acacttatca atgaatgttg
     3901 gtaagtgttt tttctttttc aaaaaaatgt ggaaaaatta cacactcttc cgtttttttg
     3961 taagaagccg gtttgatttt gttttatcga ttacctttgt ttcggattca ttaataaaca
     4021 atagaattac gcgcattgat ttttatttta atccaaccaa aatcttaact atgtttttat
     4081 ttttttcgta gtgattacgg gagcttaaaa acatagtttt tttattctta tttatcccca
     4141 acactttgtt gatctcgttt gttatatcgt aaaatgcaac cggtatgggc tacgaaatcg
     4201 aacgtaaatt cctcgtcagc ggtgaatata aatcacatgc ttacg
//
LOCUS       DAOSDR010000277         4241 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrimonas sp. isolate AMR_MDS_1826
            NODE_7360_length_4241_cov_4.216436, whole genome shotgun sequence.
ACCESSION   DAOSDR010000277 DAOSDR010000000
VERSION     DAOSDR010000277.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863355
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrimonas sp. (wastewater metagenome)
  ORGANISM  Petrimonas sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Dysgonomonadaceae; Petrimonas.
REFERENCE   1  (bases 1 to 4241)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4241)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:10:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,720
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 35
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4241
                     /organism="Petrimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7360_length_4241_cov_4.216436"
                     /isolate="AMR_MDS_1826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2023866"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..382
                     /locus_tag="PLI30_10375"
     CDS             <1..382
                     /locus_tag="PLI30_10375"
                     /inference="COORDINATES: protein motif:HMM:NF012335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HOI79997.1"
                     /translation="FGFNDKTFGGHGEYLTIAENDAVINMPENMSFDEAAAITEGSHY
                     ALVNIRAAKVERGQKVLVYGATGAIGSAAVQLLKHFGAIVTAVCNTKNVDLVRSLGAD
                     TVIDYQTQDFTKTEDKFAFIFDAV"
     gene            766..1863
                     /gene="bla"
                     /locus_tag="PLI30_10380"
     CDS             766..1863
                     /gene="bla"
                     /locus_tag="PLI30_10380"
                     /EC_number="3.5.2.6"
                     /inference="COORDINATES: protein motif:HMM:NF033088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="subclass B1 metallo-beta-lactamase"
                     /protein_id="HOI79998.1"
                     /translation="MKKVIAAINMTLDGFCDHTSGIPDDEIHQHYADLLRSADTALYG
                     RITYQLMEFWRTVLEKCKISKSRPGGRNMKILLMICCIVFVTDVVGQTVVVNPDGTHS
                     TVHGNVIVNPNGTHSTVHGNIIVNPDGTHSIAHGNVNIDRTTENRAYKSASLIINQVS
                     EHVYQHISFLDFGNFEKISCNGMIIVNNNEAVVFDTPTDNETSRELIDWLTQSLKCKI
                     IAVVPTHYHIDNLGGLEEFHRQDIISYAYNRTIQVLKENGSTMPKHGFDEHFELKVGN
                     EEVYVDFLGEGHSCDNIIAYFPLEDIMFGGCLIKEAGAGKGNLAEANVEEWPNTVRKV
                     KMKYPEVKKIIVGHGKSGGIELLDYTIKLFE"
     gene            1982..2683
                     /locus_tag="PLI30_10385"
     CDS             1982..2683
                     /locus_tag="PLI30_10385"
                     /inference="COORDINATES: protein motif:HMM:NF025687.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4386 family protein"
                     /protein_id="HOI79999.1"
                     /translation="MEETEYKSNEELNWNKLYKVGGVAAIVIVLIIPIQLVIFTLFPP
                     PETTMGFFELFRKNWLIGLLSLDLLYYINNGILILVYLGLFAALRKVNFTNMLIATII
                     GLTGIAVYYVSAVGFEVLSISKQYFSAESMEYKQQLLAVGQGLIARYKGTAFDVYYVF
                     NAITLILISMTMYKSRDFKRSSATWGLIAGIFMIIPSTAGTLGLICSLLSLVPWIVFS
                     VLIGRRLIQMAKRQV"
     gene            2699..3154
                     /locus_tag="PLI30_10390"
     CDS             2699..3154
                     /locus_tag="PLI30_10390"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HOI80000.1"
                     /translation="MKSKMMKYPEPTVGAIIFNPDNKVLLCKAHKWNDKYIIPGGHIE
                     LGEKMEEALKREILEETGLKIYDIHLISIKESVYNDSFHEKRHMIFIDYICKTDSYDV
                     SLTDEAQEYEWVDLKDIDNYELGGFLKPFFEELKNKKESRHKTEILYNY"
     gene            3260..3505
                     /locus_tag="PLI30_10395"
     CDS             3260..3505
                     /locus_tag="PLI30_10395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI80001.1"
                     /translation="MRQLLIWREPLSKHGMMVIPHPISTLPTKNGSTETMAVLTYNLL
                     SYSGEAIWKENYRKTAILHGTSHVFSKYFLNFVHYTC"
     gene            3520..>4241
                     /locus_tag="PLI30_10400"
     CDS             3520..>4241
                     /locus_tag="PLI30_10400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006842549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF853 family protein"
                     /protein_id="HOI80002.1"
                     /translation="MFDNSKRAFIAINDEAEVCLIPKMANRHGLITGATGTGKTVTLQ
                     TLSETFSEMGVPVFAADMKGDLSGVAKTGGNKESVSKRVDGYKLGKKGFEFKGFPVRF
                     WDVFGEQGHPVRTTVTEMGPMLLERLLQLNETQGAVLTMVFKIADDNNLLLLDLKDLQ
                     KMIQFVGDNRAKYTTEYGNISPASIGAIQRALLRLESEGADKFFGEPELVITDFMQTE
                     QGRGVINILAADKLMNSPRVYTT"
BASE COUNT         1414 a          786 c          931 g         1110 t
ORIGIN      
        1 ttttggcttc aatgataaaa cctttggtgg gcacggagaa tatctgacaa tcgctgaaaa
       61 cgatgcagtt ataaatatgc cggagaatat gagttttgat gaagcggcag ctattacgga
      121 agggtctcat tatgccttag taaatattcg ggcagcaaaa gtagaacgcg gacaaaaagt
      181 tctagtctac ggagcaacgg gggctatcgg ttctgcagcc gtacaattat taaaacactt
      241 tggagcaata gtaactgctg tctgtaatac aaaaaacgta gatctagtga gatctctcgg
      301 tgcagacaca gtcattgatt atcagacaca ggattttaca aagacagaag ataagtttgc
      361 tttcatcttt gacgcagtgt gaaaaagttc atttggtaag tgcaaacctt tattaactga
      421 aaaaggaata tacatctcaa cggaatttgg gaaaaacgca gaaaatatat tatttgcatt
      481 gaccacacca ctttggggag gtaaaagact tttgtttcca attccgtcta tcaccaagca
      541 agatataata tttttgaaag agcttgttga aaaaggtgaa ttcaagcctg taatagacag
      601 attttatacg ttaggccaaa ttgtggatgc ttacagatac gttgaatcgg ggcaaaaaac
      661 aggtaatgtt attttaaaga tacgtgaatg ataagaacac tagccactaa caaccgttac
      721 tagtcattgt aggacgattc acaaaacaga ctgacaaaaa aaattatgaa aaaagtaatt
      781 gcagcaatca atatgacgct tgacgggttt tgtgaccata cctcaggtat ccctgacgat
      841 gaaatacatc agcattacgc tgacctcttg agaagtgctg acacagcgtt atacggaagg
      901 ataacctacc agcttatgga gttttggcga accgtgctgg aaaagtgcaa aattagcaag
      961 tcaaggcctg gaggaagaaa tatgaaaatt ttattgatga tttgttgtat tgtatttgtg
     1021 acagatgtgg taggacagac agttgtagta aatccagacg gaacgcattc tactgttcat
     1081 ggcaacgtaa ttgtaaatcc aaacggaacg cactctaccg ttcatggcaa cataattgta
     1141 aatccggatg gaactcactc tatagctcat ggcaatgtca acatagatcg aacaacggag
     1201 aacagggcat acaagtcagc gtctttgata atcaatcagg tttcggagca tgtatatcag
     1261 catatttcat ttcttgactt tgggaacttt gagaaaattt cgtgcaacgg aatgattatt
     1321 gtgaacaaca atgaagctgt tgttttcgat acaccgacag acaatgaaac atctcgcgag
     1381 ctgattgact ggttaacaca atcgttgaaa tgcaaaatta tagcggttgt tccaacgcat
     1441 tatcatatcg acaacttggg aggcctggag gaatttcaca gacaggacat tatttcttac
     1501 gcctacaaca gaacgataca agtactgaaa gaaaatggtt ctactatgcc gaagcacggg
     1561 tttgatgaac attttgaatt gaaagtcggc aacgaagaag tgtatgttga ttttctggga
     1621 gaaggtcatt cctgcgataa cattattgct tatttccccc tggaagatat tatgtttggg
     1681 ggttgtttaa taaaagaagc aggagcgggg aaaggtaatc ttgcggaagc aaatgtagaa
     1741 gagtggccga atacagtaag aaaagtaaag atgaagtatc cagaagtaaa aaaaattata
     1801 gtcggacacg ggaaatccgg cggaatcgag cttttggatt atacgattaa gctgtttgaa
     1861 tgaaaaaatt gccgctgata tagcctacac tgttccgcgc aagaataaga cgacataaga
     1921 aaccatatga ttctttggat aaatagcggc ctttgtaaac ggaacaaaca aaataacaaa
     1981 aatggaagaa acagaataca agtccaacga agagttaaat tggaataagt tgtacaaggt
     2041 tggcggagtc gcagctatag taatcgtttt aataataccg atacaactgg ttatattcac
     2101 cctctttcct ccccctgaaa ccacaatggg attttttgaa ctgttccgta aaaattggct
     2161 cattggatta ttgagccttg atctcttgta ctatataaat aacgggattt taatcctggt
     2221 gtaccttgga ttatttgcag cattaaggaa agtaaatttc accaatatgc ttatcgccac
     2281 gataatcgga ttaacaggaa ttgcagttta ttatgtatct gccgttggat ttgaagtgtt
     2341 atcaatcagc aaacaatatt tctccgcgga atcaatggag tataaacagc agttgctggc
     2401 ggtagggcaa ggcttgatag caagatataa aggaacggca tttgatgtat attatgtgtt
     2461 taatgctatt acgctcattt tgatttccat gacaatgtat aaatcaaggg attttaaaag
     2521 atcttcagcc acgtggggcc tgattgcagg gatttttatg ataattccct cgacagcagg
     2581 gaccctggga ctgatttgct cattactctc tcttgtcccc tggattgtct tttctgtctt
     2641 gatagggaga agactcatac aaatggcaaa acgacaggtc tgaatttgaa ataaaaaatt
     2701 gaagagtaaa atgatgaaat atcccgaacc tactgtgggg gcaattattt ttaatccgga
     2761 taataaggta ctgctttgca aagcccataa atggaatgat aaatatatta ttcccggagg
     2821 ccatatcgaa ttgggagaaa aaatggaaga agcattaaaa agggagatat tggaagaaac
     2881 cggcctgaaa atttacgata ttcacctgat cagtataaaa gagagtgtgt ataatgattc
     2941 ttttcatgag aaaagacaca tgatatttat tgattatata tgtaagacag attcatatga
     3001 tgtgtcttta actgatgaag ctcaagaata tgaatgggtg gatttaaaag atatcgataa
     3061 ctatgaactg ggaggatttc ttaaaccgtt ctttgaagaa ctaaaaaata aaaaggaatc
     3121 gagacataag actgagattc tttataacta ctaacatgaa gatacggata aacaattatc
     3181 tggaaacctc cattgcccgg agaaaaacct gaacaactat caactaaata taaaaacatc
     3241 atggaaaaac gtaccattag tgagacaatt attgatttgg agagagccgc tctcgaagca
     3301 tggcatgatg gtaattcctc accctatttc gaccctgccc acgaaaaacg gatcgaccga
     3361 aacgatggct gtcctgacct ataacctgct ctcatattca ggtgaggcaa tctggaaaga
     3421 gaattatcgg aaaaccgcca ttcttcacgg aacttcacac gtcttttcca aatatttcct
     3481 taactttgtc cattatacct gctaaaactt tacaaaccta tgttcgacaa cagcaaacgc
     3541 gcttttatag ccatcaacga cgaggctgaa gtttgcctta tccccaaaat ggcaaaccgc
     3601 cacggactaa taacaggtgc cacaggaaca ggaaaaaccg tcaccttgca aacactttct
     3661 gaaacattca gcgaaatggg agtacccgtc tttgccgccg acatgaaagg cgacctctcc
     3721 ggcgtggcca aaacaggcgg aaacaaggag agcgtttcca aacgcgtgga cggttataag
     3781 ttaggtaaaa aaggatttga gtttaaaggc ttcccggtga ggttctggga cgttttcggc
     3841 gaacaggggc atccggtgcg gacaaccgtt accgaaatgg ggccgatgtt gctcgaacga
     3901 ctgctgcaac tgaacgaaac tcagggagcg gtactgacta tggttttcaa aattgccgac
     3961 gataacaacc tgctgctcct cgacttgaaa gacttgcaaa agatgatcca gttcgtagga
     4021 gacaaccggg caaaatacac caccgaatac gggaacatct ctccggcaag catcggcgcc
     4081 atacaacgcg cgctgctacg cctggaaagc gaaggcgccg acaaattctt cggtgagccc
     4141 gaactggtga ttaccgattt tatgcaaacg gagcaaggaa gaggagtaat caacattttg
     4201 gccgccgaca aactgatgaa ctccccacgg gtgtatacca c
//
LOCUS       DAOSDR010000278         4222 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrimonas sp. isolate AMR_MDS_1826
            NODE_7398_length_4222_cov_3.687305, whole genome shotgun sequence.
ACCESSION   DAOSDR010000278 DAOSDR010000000
VERSION     DAOSDR010000278.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863355
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrimonas sp. (wastewater metagenome)
  ORGANISM  Petrimonas sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Dysgonomonadaceae; Petrimonas.
REFERENCE   1  (bases 1 to 4222)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4222)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:10:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,720
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 35
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4222
                     /organism="Petrimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7398_length_4222_cov_3.687305"
                     /isolate="AMR_MDS_1826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2023866"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1282)
                     /locus_tag="PLI30_10405"
     CDS             complement(<1..1282)
                     /locus_tag="PLI30_10405"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOI80003.1"
                     /translation="MDEELDDISSLVEKYEQMSMFGRKIYFDADEFAVLADHYNNLGD
                     NELAEEIIEEGLKMHPASPELMILKAKTLVYSELYDEALSYLNNIPGEGDIELPLLRI
                     ESLLHLEKTDEANEVINETMNRELSIDDLYVFITEVGYVMNDVEHFDRAISYLEESLK
                     IDESNPDVLIDLAYANEMKGDFARAIEYNNRLLDLDPYSFEGWVNIGKLYSMNEQYEK
                     AIDSFDFALTINENDVSALKMKALSLYLNDNAPEAIRIFEECIRISPDDESLYDSLLE
                     GYEAMEQYDQMMRVIDIKEKRFGPKGIATRRAFVELLKDNMASARELYNNIPADEKES
                     LDYYMLEGELMFIDGDLRGAEAAYIKAALLSEDNEEIIDRLANVSVAQEKYEQAAEYL
                     EQLLKIDSDYPTAKSRLAFIRFEIGNKEPFDEIMK"
     gene            complement(1284..2981)
                     /locus_tag="PLI30_10410"
     CDS             complement(1284..2981)
                     /locus_tag="PLI30_10410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004819 - glutamine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006425 - glutaminyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase/YqeY domain fusion
                     protein"
                     /protein_id="HOI80004.1"
                     /translation="MSQKEIPAVEENKKSLNFIEQIVENDLKEGKNDGRVQTRFPPEP
                     NGYLHIGHAKAVCIDFGIAERYGGICNLRFDDTNPVKEDVEYVDSIMEDIQWLGFQWK
                     NVFYASDYFQQLWDFAVQLIREGKAYIDEQSAEEIARQKGTPTVPGTPSPYRERPVQE
                     SLDLFQKMNSGDVPEGKMVLRAKIDMASSNMHFRDPILYRVLHIPHHRTGTAWKAYPM
                     YDFAHGQSDYFEGVTHSICTLEFVVHRPLYDWFIDQLATDDYRPHQYEFNRLNMTYTM
                     MSKRKLLQLVQEKLVSGWDDPRMPTLCGLRRRGYTPQSIRNFVDSIGYTKYDGMIDVS
                     LLEFAVREDLNKKAVRVSGVIDPVKLILTNYPEGQTEEMEAINNPEDESMGSRRVKFS
                     RELWIERDDFMEDAPRKYFRLTPGNEVRLKNAYIVKCTGCKKDENGNVTEVYAEYDPQ
                     TRSGMPEANRKVKGTIHWVSVPHAIDAEVRLYDRLFIVEDPAGEKEKDFRELLNPDSL
                     IVKKNCKVEEYLAGAAPLDSFQFQRIGYFNVDTDSTGEKLVFNRTVALKDSWKKQNS"
     gene            complement(3013..4029)
                     /gene="tsaD"
                     /locus_tag="PLI30_10415"
     CDS             complement(3013..4029)
                     /gene="tsaD"
                     /locus_tag="PLI30_10415"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005841268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HOI80005.1"
                     /translation="MITILGIESSCDDTSAAVIRDGVILSNVIASQAVHERYGGVVPE
                     LASRAHQQNIIPVVHDALKQAGVTKQEISAVAFTRGPGLLGSLLVGTSFAKGLSSALN
                     IPMVEVNHLQAHVQAHFIKETPDDTNQPEFPFLCLLVSGGNSQIILVKSHRQMEIIGQ
                     TIDDAAGEAFDKCAKVMGLGYPGGPVVDKLAKQGDPGRFTFSKPHIAGYDYSFSGLKT
                     SFLYFLRDELKTDPGFIGKNKEDLCASLQKTIVDILMDKLYRASKELKIKEVAVAGGV
                     SANSGLRAAFEDYSRRYGWKIHIPKFAYTTDNAAMVAISGYYKYLDGEFCGKDVAPYA
                     RVSI"
BASE COUNT          966 a         1100 c         1002 g         1154 t
ORIGIN      
        1 gtttcatgat ttcatcgaaa ggttctttgt ttccgatctc gaaacggatg aaggccaagc
       61 gcgattttgc cgtcggataa tcggagtcga tcttcagcaa ctgttccaga tactccgcag
      121 cctgctcgta tttctcctgg gcaacactca cgttggccag gcggtcaatg atctcctcgt
      181 tgtcttccga caatagcgca gcctttatat atgccgcttc cgcccctcgc aaatcgccgt
      241 cgataaacat cagctctcct tcgagcatgt agtaatcgag cgattccttt tcatcggcgg
      301 gaatgttgtt gtataattcc cgggctgaag ccatattgtc tttcaacagt tcaacgaatg
      361 cccggcgcgt agcgatccct ttcggtccga aacgcttttc cttgatatcg ataacgcgca
      421 tcatctggtc gtactgttcc atggcctcat acccctcgag tagcgaatca taaagcgact
      481 cgtcatcggg cgatatgcgg atgcattctt cgaagatacg gatggcttcg ggtgcattat
      541 cattcaggta gagcgacaac gctttcatct tcaaggccga gacatcgttt tcgtttatgg
      601 tcagcgcaaa atcgaaggaa tcaatggctt tctcgtactg ttcattcatc gaatagagct
      661 tgccgatgtt tacccaacct tcaaacgaat agggatccaa gtctagcaac cggttgttgt
      721 attctatggc tcgcgcgaaa tcgcctttca tctcgttggc atatgccaga tcgataagca
      781 catcgggatt ggattcatca atcttcaggc tctcctcaag gtaggaaatg gcccggtcga
      841 aatgctccac atcgttcatc acataaccaa cctcggtaat aaaaacgtac aaatcatcaa
      901 tggacagctc cctgttcatc gtttcattga tcacctcatt ggcttcatcc gtcttttcca
      961 ggtgcagcag gctctcaatc cgaagcaggg gaagctcgat atctccctcc ccggggatat
     1021 tgttcaggta cgacaacgcc tcgtcataca actcggagta gaccaatgtc ttggccttta
     1081 aaatcatcaa ctccggactg gctggatgca tcttgagtcc ctcctcaata atctcttcgg
     1141 ccagttcatt gtctcccaga ttgttgtaat gatcagcaag cacagcaaac tcgtcggcat
     1201 cgaaataaat tttcctgccg aacatactca tttgctcgta tttttccacc agggaagata
     1261 tgtcgtccag ttcctcgtcc attttaacta ttttgtttct tccacgaatc cttcaaggct
     1321 actgtccggt tgaagacaag tttttcgccc gtagaatcgg tatctacgtt aaaatacccg
     1381 atacgctgga attgaaagct gtctagcggt gcggcgccgg ccaggtattc ttccaccttg
     1441 cagtttttct tcacgataag cgaatcggga ttcagcagtt cgcgaaagtc cttttctttc
     1501 tctccggccg gatcctccac gataaacagc cggtcgtaaa gccgtacttc agcgtcgatg
     1561 gcatgcggta ccgacaccca gtggatggtt cctttcactt tgcggttagc ctcaggcatc
     1621 ccgctccggg tctgcggatc atactcggca tatacttccg taacgtttcc gttttcgtct
     1681 tttttgcatc cggtacattt cacgatgtag gcatttttca ggcgtacttc gtttcccggc
     1741 gtaagacgga agtatttacg gggtgcgtct tccataaaat cgtcgcgttc gatccagagt
     1801 tcgcgcgaga atttaacccg gcggcttccc atcgattcgt cctcggggtt gttgatcgcc
     1861 tccatctctt ccgtttgtcc ttcgggataa ttcgtgagga taagctttac ggggtcgata
     1921 acgcccgaca cgcggacggc ttttttgtta aggtcctcgc gcacggcaaa ctcaaggagc
     1981 gacacgtcga tcatcccgtc gtatttggta tagccgatac tatcgacaaa gttgcgtata
     2041 gattgtggtg tgtagccgcg gcggcgcagt ccgcaaagcg tgggcatgcg cgggtcgtcc
     2101 catcccgaaa ccagcttctc ctgtacgagc tgaagcagct tgcgcttgct catcatggtg
     2161 taggtcatgt tcaggcggtt gaactcgtac tggtgcggac ggtaatcatc ggtggccagc
     2221 tggtcgataa accaatcgta aagcgggcgg tgtaccacaa attccagggt gcagatagaa
     2281 tgggtcacgc cttcgaaata gtcggattgc ccgtgcgcaa aatcatacat ggggtaggct
     2341 ttccaggcag tccccgtacg gtggtgcggg atatggagga cccggtatag gatggggtca
     2401 cggaagtgca tgtttgacga cgccatatct attttggcgc gcagcaccat ttttccttca
     2461 ggaacgtcac cgctattcat cttttgaaac aggtcgagcg attcctggac gggacgttcg
     2521 cggtaagggc tgggcgtccc gggaacggtt ggcgtccctt tttggcgcgc aatctcttcg
     2581 gccgattgct catcgatata ggcttttcct tcccggataa gctggacggc gaaatcccat
     2641 aactgctgga agtaatcgga ggcgtaaaac acgttcttcc actgaaatcc gagccactga
     2701 atatcttcca taatggaatc cacgtattca acgtcttcct tgaccggatt ggtatcatcg
     2761 aaccggaggt tgcaaatgcc accgtatctt tcggcaatac cgaagtcgat acacacggct
     2821 ttggcgtgcc cgatatgcag gtacccgttg ggttccggcg gaaagcgggt ttgcacacgg
     2881 ccgtcgtttt taccttcttt taaatcgttt tcaacaatct gttcaatgaa gttgagactt
     2941 ttcttatttt cttccacagc aggaatctct ttttgtgaca taatatatgg ttggttaatt
     3001 tttaaaatac tgttatatgg ataccctggc gtaaggcgcc acgtcttttc cgcaaaactc
     3061 accgtcgaga tacttgtagt accctgagat agctaccata gccgcattat cggtagtata
     3121 agcaaatttc gggatatgta ttttccatcc ataacggcga ctataatctt caaaagcagc
     3181 gcgcaagcct gaattggcag acacaccgcc cgccacggcc acttccttta tttttaactc
     3241 ttttgacgcg cggtacaact tgtccatcaa tatatcgacg atcgtttttt gcagggacgc
     3301 gcagagatct tccttgtttt tcccgataaa gccgggatcg gttttcagtt cgtcgcgcag
     3361 gaaatagaga aacgatgttt tcaggccgct gaagctataa tcgtatcccg cgatatgggg
     3421 cttactgaaa gtaaaccggc cgggatcgcc ctgtttggct aatttatcca cgacaggccc
     3481 tccgggataa cccaggccca tcaccttggc gcatttgtcg aaggcttcac cggcggcatc
     3541 gtcgatggtt tgcccgataa tctccatctg ccggtgcgat ttcacgagaa taatctgtga
     3601 gttaccgccc gaaaccagca agcacaaaaa cgggaactcc ggttggttcg tatcgtccgg
     3661 agtttctttg atgaaatggg cttgtacgtg tgcttgcaaa tggttcacct ccaccatcgg
     3721 gatattaaga gcagaggaga gccctttcgc aaaggaggtt cctaccaaca acgagcccaa
     3781 caatcccggc ccgcgcgtga aagccaccgc cgaaatttcc tgttttgtga cgcccgcttg
     3841 tttcagggca tcgtgtacca cgggaatgat gttctgctga tgtgcgcggg atgccagctc
     3901 gggcaccacg ccgccatacc gctcgtgtac tgcctgcgag gcgatcacgt tcgacaaaat
     3961 gacaccgtcg cggataaccg ctgccgaggt gtcgtcgcag gaagattcta tacctaatat
     4021 cgtaatcatt ctatccggtt gtaattcact tagggacgta aaacaaataa cttataacaa
     4081 caaacttgtt ctttttgcat tttacaatga caacttattt atttcacatc ccttactccg
     4141 tttgtgtaat ccgttgcggc tatgaattga ataccgccaa acgaattata atcgggttac
     4201 ggtgtaaccg ataacaaagt gc
//
LOCUS       DAOSDR010000279         4220 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrimonas sp. isolate AMR_MDS_1826
            NODE_7402_length_4220_cov_2.848499, whole genome shotgun sequence.
ACCESSION   DAOSDR010000279 DAOSDR010000000
VERSION     DAOSDR010000279.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863355
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrimonas sp. (wastewater metagenome)
  ORGANISM  Petrimonas sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Dysgonomonadaceae; Petrimonas.
REFERENCE   1  (bases 1 to 4220)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4220)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:10:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,720
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 35
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4220
                     /organism="Petrimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7402_length_4220_cov_2.848499"
                     /isolate="AMR_MDS_1826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2023866"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(146..1027)
                     /locus_tag="PLI30_10420"
     CDS             complement(146..1027)
                     /locus_tag="PLI30_10420"
                     /inference="COORDINATES: protein motif:HMM:NF014625.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HOI80006.1"
                     /translation="MKYTVVFILFLFSCSLSYSQNSQINQDNRINVIVFGAHPDDPDS
                     KVGGTAIKFAKLGHNVLFVSLTNGDAGHQSMGGGALAKRRRAEAMEAGRRLGVTYKVL
                     ENHDGELLATLDLRKEVIRLIRQWNADIVIGPRPYDYHPDHRNAAIVIQDAAYMVIVP
                     NVVSDTPPLKKNPLFLYKQDRFMKPYPFKPDITIDITDVIDQKILAMTAHESQYFEWL
                     PWTSSIEAPSDKKGRVQWLKIFRELPITPSIRESLVKWYGEEVGNSITAAEAFEICEY
                     GKQPTEEEIRELFPMLK"
     gene            complement(1067..2548)
                     /locus_tag="PLI30_10425"
     CDS             complement(1067..2548)
                     /locus_tag="PLI30_10425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019245610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOI80007.1"
                     /translation="MSSNISRRKFIQSGTTVAAGLTVVPSAVLGKSKGHISPSDKLNI
                     AGVGVGGRGFTVLRGMESQNIIALADVDWNYSKRVFDYFPNAKRYYDYRKMYDELGKS
                     IDAVMIATSDHTHAVIAADAITMGKHVYVEKPLTHSVYESRLLTRLAEKFKVATQMGN
                     TAASGPGVRQIIDWIWDGQIGEVRRVDAYTDRPIWPQGLQRPMQKESIPETLKWDLFI
                     GPAPMRPYNSIYTPWNWRGWWDFGTGALGDMACHILNPVFKGLNLLYPTKVQGSSTML
                     MAESCPAAQMFKYVFPARDNMPKVGMPEVEITWYDGGLQPFRPDGMPIGKSLNVGGGA
                     AIFYGSKDTLICGNGGREPWLISGRTPNSPKAQREITISHEMDWVRACKESPEHRSKP
                     ASDFSIAGPLNEMVVMGVLAVRLQGLNQELIWDGENMKFTNIPEDATVKSIIEDGFKI
                     SDGHPTFNKTWTEPQKAEVFAQELIKHTYYNGWKLPDMPQGRS"
     gene            complement(2664..4145)
                     /locus_tag="PLI30_10430"
     CDS             complement(2664..4145)
                     /locus_tag="PLI30_10430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005650612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HOI80008.1"
                     /translation="MKKILQLGSMVFIFLIIYSCSDFLDLVPKDRLSEEVYYETPEQL
                     EFALNGAYSILRSNGVTKNWYVLAEIPSDNTYGVLSGTVTDRDEFDKFYLRSLNPFLS
                     GFWDDAYFGINRANKVIEELIDSDVEESLKNRYTLEAKFLRAFLHFNLVRIFGDVPLM
                     TKTVTIPESYNVLRTKKNEVYAQIIADLTDATALPKSYSSPADIGRATSGAAKALLGK
                     VYLTMGDYSKAAVPLKEVIDSKQYRLIENNTPGTCDGFEKYFDPNNHNNEEAIFEAQF
                     KKGGFGTGSGWVNSFSPGASGDNVVAVGGSGDDNIPEEGMWESWDEGDFRRDFSMALG
                     YTNKKTGQWVVARHVRKYRDIPYANGDANNNWPFLRYADVLLMYAECLIQTGDLTSAA
                     EYINQTRRRGFGYQSTEVGSPADIPAVSKDQLFLAVEKERRNELAFEGHRWFDLVRTG
                     RALTVLRATGQKLNETNFIAPIPQNQVDVNPNIEQNDYRIEPK"
BASE COUNT         1230 a          937 c          742 g         1311 t
ORIGIN      
        1 cgcacactct tttgacaaga aaacagcaaa aaaaatatta aaaaaattat aatagtttct
       61 tttgatttca taatactgtg aaaattttta atttaactta atattccaat tgattattac
      121 ctcgtgattg ataaaatcaa agttactatt tcaacatagg gaacaactcc ctaatctcct
      181 cttcggtagg ttgttttcca tattcacata tttcaaatgc ctcagcagct gtaatggaat
      241 ttcctacttc ttctccgtac catttaacaa gagactctct gatggaggga gtgatgggta
      301 gttcacgaaa tatcttgagc cactgaaccc ttcctttttt atcactgggc gcctcgattg
      361 aactagtcca tgggagccac tcaaaatatt gagattcatg tgcagtcata gccaatattt
      421 tctgatctat cacatccgta atgtcaatgg tgatatcagg tttaaaagga taaggcttca
      481 taaaacggtc ttgtttataa aggaacaaag ggtttttttt caatggaggt gtatcagaca
      541 ctacgtttgg aacaataacc atgtaggctg catcctgtat gacaattgct gcatttctgt
      601 gatccggatg ataatcatat ggccggggac caataactat gtcagcattc cactgcctga
      661 tcaatctgat cacctctttc ctcagatcca gggtggcaag cagttcacca tcatgatttt
      721 ccagaacttt gtaagtaact cctaatcgcc tgccggcttc catagcctcg gctcttcttc
      781 tttttgccaa tgcgcctcct cccattgatt gatgtccagc atcaccattt gtgagcgaaa
      841 caaagagtac attatgtcca agctttgcaa acttaattgc agtgccgcct actttactgt
      901 caggatcatc agggtgtgct ccaaaaacta tcacattgat tctattatcc tgattaatct
      961 ggctgttctg actgtaactc agagaacatg aaaatagaaa taaaataaat acaaccgtat
     1021 acttcatgaa tatttatttt taattaatta ctttcacttt acctacttac gacctcccct
     1081 gtggcatgtc tggtaatttc caaccgttat aataggtgtg tttgatcaat tcctgtgcga
     1141 acacctcagc tttttgaggt tctgtccatg tcttattaaa agtagggtga ccatccgaga
     1201 ttttgaagcc atcttcaata atggacttaa ctgttgcatc ctcaggaata ttggtgaact
     1261 tcatattttc accatcccaa attaattctt gatttaatcc ttgcaagcga acagcgagaa
     1321 cgcccatcac aaccatttcg tttaaaggtc ctgctataga aaaatctgaa gcaggctttg
     1381 accggtgttc ggggctttcc ttgcatgcac gtacccaatc catttcatgt gaaattgtta
     1441 tttctcgttg tgctttagga gaatttgggg ttcgtcctga gattagccaa ggttctcttc
     1501 ctccatttcc acaaattaaa gtatcctttg aaccataaaa aattgcagca ccacctccaa
     1561 catttaaact tttaccaatt ggcattccat cgggtcgaaa tggttgtaaa cctccatcgt
     1621 accaagtaat ttccacttcg ggcataccta ccttaggcat gttatcacga gcaggaaaaa
     1681 catatttaaa catttgtgca gcaggacaac tttcggccat tagcatagtt gaagaaccct
     1741 gtacttttgt tggatataat agatttaatc ctttaaatac aggatttaaa atatggcaag
     1801 ccatatcgcc cagcgctcca gttccaaaat cccaccagcc acgccagttc cacggtgtat
     1861 atatactgtt gtatggtctc atgggggcag ggcctataaa cagatcccat tttaacgttt
     1921 cggggattga ttctttttgc ataggacgtt gcaatccctg aggccaaata ggcctgtctg
     1981 tataagcgtc aactcttcta acttcaccaa tttgaccatc ccaaatccaa tcgataatct
     2041 gacgaacacc aggcccggag gctgcagtat tgcccatttg agtagcaact ttaaatttct
     2101 ccgccaatct tgttagtaat cgcgattcgt aaacactatg tgtcaacggt ttttcaacgt
     2161 atacatgctt acccattgtt atggcatcag ctgcaataac cgcatgggta tggtcagatg
     2221 tagcaatcat cacagcatct atgctttttc ctaactcatc atacatttta cgataatcgt
     2281 agtatctctt ggcatttggg aagtaatcaa aaacacgttt gctatagttc caatccacat
     2341 cggcaagcgc tataatgttc tgactctcca tgcctctaag cacagtgaat cctcttcctc
     2401 caacaccaac acctgcgata tttaatttat cagacgggga tatgtgtccc ttactttttc
     2461 ctaatacagc tgaggggact acagttaacc ctgcagctac agttgtaccg gactgaatga
     2521 attttcttct tgaaatgttt gacgacataa ttttttttca atgttatata aattgcaaat
     2581 aactgtctaa ttttttcata atcaatcaga cagttattta caaagaatag cgaatacgct
     2641 ctaagattta gaaaacaggt attctatttt ggttcaatac gatagtcatt ttgctcaatg
     2701 ttcggattta catcaacctg attttgcggt atcggggcaa tgaaattagt ttcgtttaac
     2761 ttttgacctg tggctctcaa cacggtaagt gcacgtccag tccgtaccaa atcaaaccat
     2821 cgatgaccct cgaatgccag ttcgttacgg cgttcttttt caactgccaa aaaaagctga
     2881 tctttactga cagcgggaat atccgccgga gaacccactt ctgtgctttg ataaccaaaa
     2941 ccacgacgac gagtttgatt aatatattca gctgctgaag ttaagtcgcc agtctgaatt
     3001 aaacattctg cgtacatcaa aagaacatcc gcatacctaa gaaatggcca attgttatta
     3061 gcatcaccat tggcataagg tatgtcccta tatttacgaa cgtgacgggc tactacccat
     3121 tgtcctgttt ttttattcgt ataccccaaa gccatagaga agtctctgcg aaaatcacct
     3181 tcgtcccaag attcccacat tccttcttca gggatattgt catcaccgga accaccaaca
     3241 gctactacat tatcacccga agcgccagga gagaacgaat ttacccagcc acttccggta
     3301 ccgaacccgc ctttcttaaa ttgagcttca aaaatggctt cttcattgtt atgattgttg
     3361 gggtcaaaat atttctcaaa tccatcacag gttccgggtg tgttattttc aatcaggcga
     3421 tactgcttgg aatcaataac ctctttgagg ggcacggctg ctttggaata atctcccatg
     3481 gttaggtata ccttgccgag tagtgccttt gcagctcctg atgttgcccg gccaatatca
     3541 gccggcgaag agtacgactt aggaagagct gtagcatcag tcaagtctgc tataatctgt
     3601 gcatatactt catttttttt cgtacgtaac acgttgtaag attcaggaat agttacagtc
     3661 tttgtcatta aggggacatc cccgaaaata cggactaagt tgaaatgcag aaaggctcgc
     3721 aaaaactttg cttccagagt atagcggttt tttaatgatt cctctacatc tgagtctatc
     3781 agttcctcta tgactttatt agcccgattg ataccaaaat aagcatcatc ccaaaaaccg
     3841 gataaaaatg ggtttaatga acgaagataa aacttatcga attcatctct gtctgttact
     3901 gtaccagaca acactccata tgtattgtcc gaaggtatct cagccaatac gtaccaattt
     3961 tttgtcacgc cattgcttct gagtatagaa taagcaccat ttaaagcgaa ctccaattgt
     4021 tcgggtgttt cataatacac ctcttcgctt aacctgtctt taggaacaag atccaagaaa
     4081 tcggaacaag aatatatgat aaggaagata aaaaccatac ttcccaattg caatattttt
     4141 ttcataggaa taatttttat aattaattat atcaaaaagt tatatttaac ccaaagctat
     4201 aagacttgga cgttggataa
//
LOCUS       DAOSDR010000280         4212 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrimonas sp. isolate AMR_MDS_1826
            NODE_7419_length_4212_cov_4.405581, whole genome shotgun sequence.
ACCESSION   DAOSDR010000280 DAOSDR010000000
VERSION     DAOSDR010000280.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863355
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrimonas sp. (wastewater metagenome)
  ORGANISM  Petrimonas sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Dysgonomonadaceae; Petrimonas.
REFERENCE   1  (bases 1 to 4212)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4212)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:10:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,720
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 35
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4212
                     /organism="Petrimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7419_length_4212_cov_4.405581"
                     /isolate="AMR_MDS_1826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2023866"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            2..451
                     /locus_tag="PLI30_10435"
     CDS             2..451
                     /locus_tag="PLI30_10435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019538294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HOI80009.1"
                     /translation="MAKSIRNLFDDNADLYNVDTIKIDDIKPYKYLILGTSTTGIGDL
                     QDDWDSFLPVFSKADLSDKTVAIFTLGDSASFSSSFAEAMKVIYDAIKDKTKVVGFVD
                     DEGYTYDDSTAVVDGRWVGLPIDEDNEYDQTDARLKAWVEELKKEFI"
     gene            576..3371
                     /locus_tag="PLI30_10440"
     CDS             576..3371
                     /locus_tag="PLI30_10440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014224313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HOI80010.1"
                     /translation="MQRFFYYRPFVFITLLTLSLSSFSQKKQLDHSVYDNWKSLQEIS
                     ISNDGRFINAVISPQEGDSTLYIYDSKKEKELLIHRVNKYTLSPDGRYTVALLKAPFS
                     EIRQAKIKKKKADDFPKDSLVIVDNEAFTLYKIADVKSYATSTEMAGHIAYKKAAPKD
                     TAKNKPNKPADLLIIRNLNTSAEDTVKNSKEFAFNKFGNSLAVSVEPEKKDSTDTHKV
                     LFFDLKNGNKKQISGEKMEYRSFSFDEPGNQLVYLATKDTSKIEQKVFDVRYFKNTMD
                     SAVVIASKTSRGLPENWIFNENSKPSFSKNGQRILVGAAPRQTPKDTTLVDFETAALD
                     IWHWKDPVVQPQQLSQLKNELRRTYTGIIDPNRPREFISVANEQMPNASFSDEGNGRF
                     VLLTSGLPYEIESQWDISSKMDTWIYDTQSNQLTVIAQPVSGRPQISPSGNFTYWWNA
                     SEKQWFAFDNKTGKTIGLTQEIPVNFWNEKNDTPSEPGAYGIAAWGEGDKFVLMYDAF
                     DIWKLDPSGKQKPVNITNNAGRTDSITFRYINTDPDKRFVENKDVLLLSAFDNISKEA
                     GFYTLRLQGRNPLLKRELDKYGYTSVKKAKNTDLFVFQKSNFNTSPDLYITQNLWQSA
                     KKLTDINPQMKEYNWGTTELIRWTAFDGKQSQGILYKPENFDPSKKYPVMIYFYEKHS
                     DELYRYFAPAPSRSVINIPFFVSRGYIVFTPDIHYGVGQPGPDAYNYVVSGAQALAKN
                     QWVDSENMAIQGQSWGGYQVAYLITQTNMFKAAGAGAPVSNMTSAYGGIRWETGRSRQ
                     FQYEQTQSRIGATMSDSLQLYIENSPVFFANKVNTPLLIMHNDNDGAVPWYQGIEYFM
                     SLRRLGKPVWMLQYNKEAHNLNQRRNAKDLSIRLQQFFDHYLKGAPAPVWMTRGLPAT
                     EKGKSWGYDLEAGQPGN"
     gene            3400..3978
                     /locus_tag="PLI30_10445"
     CDS             3400..3978
                     /locus_tag="PLI30_10445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019539447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma-70 factor"
                     /protein_id="HOI80011.1"
                     /translation="MTSEVEELIALKNGSHKAFEAIYNRYAGKLYNFIMTISHGDKYM
                     SEEVIQSAFVRLWETKERIDPEKSILFYLSTISRNLLFNRYQRQTIEFLYQKLMINEQ
                     KPYHRQTENEIDRKWLESFVDELIDQLPPARKKIFILRKKEDLSTREIAEAMNISVST
                     VETQLSLAMKFMKAEFAKNYDKLFLAVFFLLI"
BASE COUNT         1336 a          953 c          918 g         1005 t
ORIGIN      
        1 cgtggctaaa agcatccgga accttttcga cgataatgcc gacctttaca acgtcgatac
       61 gattaagatt gacgacataa aaccttataa atacctgatc ctgggcactt caactacagg
      121 aattggcgat ttgcaggacg actgggacag ttttcttccc gttttttcga aagccgacct
      181 atcggataaa accgttgcaa tctttacgct gggtgacagc gcctcgtttt cgagcagttt
      241 tgccgaggca atgaaagtaa tttacgatgc cataaaggat aaaacaaaag tggttggctt
      301 cgtcgacgat gagggataca cctacgatga ctctaccgct gttgtagacg gacgttgggt
      361 aggccttccc attgatgagg ataatgaata cgatcaaacc gacgcccggc ttaaggcttg
      421 ggtagaggaa ttaaaaaaag agtttatttg atcaaactgt tttaaggata cggacagggc
      481 tgagttgtac aggcgattca gccctgtttc ttttatgggg tttcccttgt tcaagttgaa
      541 gttttatcgt aatttcgttg ccaaaaataa attttatgca acgtttcttt tactacaggc
      601 cctttgtatt tattactttg cttacgctca gtctatcgag cttctctcag aaaaaacaac
      661 tcgatcactc cgtttacgac aactggaaaa gcctccagga aatttccatc agtaatgacg
      721 gcaggtttat caacgccgtt atcagcccgc aggaaggcga cagcacactt tatatctatg
      781 attcaaagaa agaaaaagag ttactaattc atcgggtaaa caaatatacg ctgtcgcccg
      841 acggacgata cacggtcgct ttgttgaaag cccctttttc agaaattcgt caggcaaaga
      901 tcaagaaaaa gaaagccgat gattttccca aagactcgtt ggttattgtc gataatgaag
      961 catttacgct ttacaaaata gccgatgtaa aatcttatgc cacatctaca gagatggctg
     1021 gacacattgc ctataaaaaa gccgcaccga aagatacggc aaaaaacaaa ccaaacaaac
     1081 cggcagacct gctaattatc cgaaacctaa acacctctgc ggaagatacc gttaagaact
     1141 ccaaagagtt tgcttttaac aagttcggaa attcactggc cgtctcagtg gagcccgaaa
     1201 aaaaagattc caccgatacg cacaaggtac tttttttcga tttaaaaaac gggaacaaaa
     1261 agcagatctc cggcgaaaaa atggaataca gatccttttc cttcgacgag ccaggaaacc
     1321 aactagtcta tttagccaca aaggacacct ccaaaattga acaaaaagta ttcgacgtga
     1381 ggtatttcaa aaacaccatg gattcggccg ttgtgattgc ttccaaaacg tcaagggggt
     1441 tgccggaaaa ctggatcttc aatgaaaact caaagccttc tttcagtaaa aacgggcaac
     1501 ggatccttgt tggcgctgct ccaagacaaa cgcccaaaga tactaccctt gttgacttcg
     1561 aaacagcagc ccttgatatt tggcactgga aggatccggt tgtacagcca caacaactct
     1621 cccagctgaa aaacgaattg cgcaggacct ataccggcat tatcgacccc aatcgtccac
     1681 gcgagttcat ttctgttgcc aacgagcaaa tgccaaacgc atcgttctcc gacgagggaa
     1741 acggacggtt tgtcctgctc acttccggct tgccgtacga aattgaaagc caatgggaca
     1801 tttcctccaa aatggatact tggatttacg atacgcaatc caatcaattg accgtaattg
     1861 cccaacctgt ttcgggaagg ccacagattt caccctcggg taatttcacc tactggtgga
     1921 atgcttcgga aaaacagtgg tttgctttcg acaacaaaac cggaaaaacc atcggcttga
     1981 cacaagaaat tccggtcaac ttctggaatg agaaaaatga tacaccaagc gaaccgggtg
     2041 catacggaat tgcagcatgg ggagaggggg acaaattcgt acttatgtac gatgccttcg
     2101 acatctggaa attagatcct tccggaaaac agaaaccggt gaacatcacc aacaatgccg
     2161 gaaggactga ttccattact ttccggtaca tcaacaccga tccagataaa cgtttcgtcg
     2221 aaaacaaaga tgtgttgctc ttatcggcgt tcgacaacat aagtaaggag gccggtttct
     2281 acacgctaag actacaaggt cgaaatccac tgttaaagag ggaactggat aaatatggat
     2341 acacttctgt taagaaggca aaaaacaccg acctgtttgt gtttcaaaaa agcaatttca
     2401 atacgtcgcc agatttgtat atcacccaga atttatggca atcggcgaaa aaactcaccg
     2461 acatcaatcc tcagatgaaa gagtataact ggggtacgac agaactaatc aggtggactg
     2521 ctttcgatgg aaagcagtcg cagggaattt tgtataaacc cgagaatttt gacccttcca
     2581 aaaaatatcc ggtaatgatc tatttctatg aaaaacattc cgatgaactt tatcgctatt
     2641 ttgcgcccgc cccaagccga tctgttatca acataccgtt cttcgtcagt cgcggctaca
     2701 tcgttttcac tcccgacatc cattacggag tgggacaacc cggccctgac gcatacaact
     2761 acgttgtttc gggagcgcag gcattggcaa aaaaccaatg ggtagattcg gaaaacatgg
     2821 ccatacaggg acaaagctgg ggaggctacc aggtggccta tctcatcacc cagacaaaca
     2881 tgtttaaagc tgcaggagcc ggagcacccg tatcaaacat gacgagcgcc tacggcggaa
     2941 tcagatggga aacgggtcgc agccgccagt tccagtacga gcagacacaa tcgaggattg
     3001 gagccacaat gagcgattcc ctgcaactgt atattgaaaa ctcccccgtg ttctttgcca
     3061 ataaggtaaa cacgccactg ctcattatgc acaacgataa cgacggcgca gtgccctggt
     3121 atcagggaat tgaatatttc atgtcactgc gacgcctggg caaaccggtt tggatgctcc
     3181 aatacaacaa ggaagcccat aacctgaacc agcgtcgtaa cgcaaaagac ctttccatcc
     3241 ggctccaaca atttttcgac cactacctga aaggagctcc cgctcctgtt tggatgacac
     3301 gtgggttgcc ggctactgaa aaaggaaaat cgtgggggta tgatctagag gccggacaac
     3361 ccgggaattg agcggcagga agatggagaa aagtagtcca tgacaagtga agtagaagaa
     3421 cttatcgcgt tgaaaaacgg ttcgcataaa gctttcgaag ctatttacaa cagatatgcg
     3481 ggaaagcttt acaacttcat catgaccatc tctcatggcg acaagtacat gtcagaagaa
     3541 gtcatccaat ctgctttcgt tcgtttatgg gaaacaaaag aaagaataga tcctgaaaag
     3601 tcaatattgt tctacttgtc cacaatctcg agaaatctat tgttcaacag atatcaacgt
     3661 caaacgatcg aatttttgta tcaaaaatta atgatcaatg agcagaagcc gtatcaccga
     3721 caaacagaaa atgagataga ccggaagtgg ctcgagagct ttgtggatga acttattgac
     3781 caacttccgc ctgcgaggaa aaagatcttt atattacgca agaaggaaga cctttccaca
     3841 agggaaattg ctgaagcaat gaacatatcc gtcagtacgg ttgaaacgca attatcgctc
     3901 gcgatgaagt tcatgaaagc cgaattcgct aaaaattacg acaaactgtt tctggcggtg
     3961 ttctttttgt taatttaatg taatgtaaaa atttgattag cggaaaagca gtacccgatt
     4021 gtatcataac agagaggcta aataaaaaat gaccgataaa aactacatgg tattgttcga
     4081 gaaatatctc cggggtgagt ccaccgccga agagacagaa gcattaatcg acctcatcgg
     4141 ctccgaagat aaattcaagg agatgttgga ggtcgaactg atgaatgcag atccaaccat
     4201 tgacgaaacg at
//
LOCUS       DAOSDR010000281         4209 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrimonas sp. isolate AMR_MDS_1826
            NODE_7425_length_4209_cov_4.400818, whole genome shotgun sequence.
ACCESSION   DAOSDR010000281 DAOSDR010000000
VERSION     DAOSDR010000281.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863355
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrimonas sp. (wastewater metagenome)
  ORGANISM  Petrimonas sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Dysgonomonadaceae; Petrimonas.
REFERENCE   1  (bases 1 to 4209)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4209)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:10:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,720
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 35
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4209
                     /organism="Petrimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7425_length_4209_cov_4.400818"
                     /isolate="AMR_MDS_1826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2023866"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..695
                     /locus_tag="PLI30_10450"
     CDS             <1..695
                     /locus_tag="PLI30_10450"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF020459.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GSCFA domain-containing protein"
                     /protein_id="HOI80012.1"
                     /translation="CMENISRMFAESAAFIPRTDVFFITFGTAYVYKLKSTGEVVANC
                     HKFPPDTFIRERLTVEAIVKEWSGVINTISALNPAARFIFTVSPIRHWKDGAHENQIS
                     KSILHLAIDELQEMFTSALTYFPAYEILLDELRDYRFFAEDMMHPSGVATDYIWERFC
                     KTFFRRETQDAISEWNQISRSLNHVPLNESTENYRQFLKQTLQKLILFRQNHPRIDCR
                     RETEELTKKIKQ"
     gene            692..3160
                     /locus_tag="PLI30_10455"
     CDS             692..3160
                     /locus_tag="PLI30_10455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005859730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     UDP-N-acetylmuramoyl-tripeptide:D-alanyl-D-alanine
                     ligase/alanine racemase"
                     /protein_id="HOI80013.1"
                     /translation="MKYSILKIASILGIKNQNIAEGEISLLLTDSRKLSSPQETLFFA
                     IETKQNDAHKFISDLYGSGVRNFVVTKLFPEWKSFTDVNFLKVTNTLHALQKLAAYHR
                     KQFSIPVIGITGSNGKTIVKEWLYQLLEEDYNITRSPRSYNSQTGVPLSVWEMTENTT
                     LAIFEAGISQPDEMKYLEPVIRPTIGVFTKLGEAHQENFSSQQQKCMEKMELFVNSNV
                     FIYEEDNKIINQSADRMVLSQKSFCWSRKDPDAPLYIRKTEKNGNKTTIHYSFLNFEY
                     SVSIPFTDEASIENAISCLAVMLYLNVKPGTISERMMKLEPVAMRLDVRQGKNNCTII
                     NDTYNSDINSIKIALDFQQQRKVEQSLKKTLIISDILQTGLLPKSLYKHVAELVQQSG
                     IEKLIGIGHDICENSEVFTVREKHFFRSTEDFIRSNIWKEFENELILLKGARRYHFEQ
                     INELLEQRIHETVLEVDLDALVHNFNFYKSRISPQTKLICMVKANGYGAGAVEIAKTL
                     QYHRCAYFAVAVAEEGIELRKEGISTPIIVLNPEVNGFEELFSKDLEPEVYNFRILDA
                     FIREAERRGITNYPIHLKIDTGMHRLGFLPAQIPELLERLKSQKGLKVKSVFSHLAAS
                     ESWMFDSFTQEQIDIIDVAHREIGEKLGYPVWKHILNSAGIERFINAQWDMVRLGIGL
                     YGVSASGLNGLKNVSTLKTTILQIQEIPNHETVGYGRKEALNRDAKIATIRIGYADGL
                     DRKSGNRKGKVWINGKYAPIVGNVCMDLCMIDITGIDAKEGDSVIVFGENLPVLEVAE
                     SIDTIPYEILTSISPRVKRIYVKE"
     gene            3182..3583
                     /locus_tag="PLI30_10460"
     CDS             3182..3583
                     /locus_tag="PLI30_10460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007748058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2721 domain-containing protein"
                     /protein_id="HOI80014.1"
                     /translation="MEQLSLTTPSLLFSAISLLMLAYTNRFLSYAQLVRNLKEKVIED
                     KSSVALPQIANLRKRLNLIQSMQVYGIMSLLLCVASMFFIYIKMYNAAGYIFGIALIL
                     LIVSLAICVREIQISMRSLNLHLHDMEEIEK"
     gene            complement(3658..3849)
                     /locus_tag="PLI30_10465"
                     /pseudo
     CDS             complement(3658..3849)
                     /locus_tag="PLI30_10465"
                     /inference="COORDINATES: protein motif:HMM:NF014620.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NifB/NifX family molybdenum-iron cluster-binding
                     protein"
     gene            complement(3863..>4209)
                     /locus_tag="PLI30_10470"
     CDS             complement(3863..>4209)
                     /locus_tag="PLI30_10470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI80015.1"
                     /translation="FNHEIIFAFGSEQLFVYSGCAHRGLLNILDSVRLSTGKKVGTLM
                     GGFHLLDSEKGRLYESPDEIDSIALALKTNYPETSFITGHCTGEKVYERLRNKLGNQL
                     NRFYTGYTTIIE"
BASE COUNT         1280 a          993 c          906 g         1030 t
ORIGIN      
        1 agtgcatgga aaacatttcc cggatgtttg ccgaatcagc agcatttatt ccccggacag
       61 atgtattctt catcactttt ggcacggctt acgtttacaa actgaaatcg acaggagaag
      121 tcgtggccaa ttgccacaaa tttccaccgg acacctttat aagggaacga ttaacggtcg
      181 aagcaatcgt aaaggaatgg agcggcgtca tcaacaccat ctcggcactt aatcctgctg
      241 caagatttat ttttaccgtt tcacccatcc ggcactggaa agacggtgcc cacgaaaatc
      301 agatcagcaa atccatcctt catctggcaa tagacgaatt gcaggaaatg tttacatccg
      361 cactcactta ctttccggcc tatgagattt tattggacga actgcgtgac taccgtttct
      421 ttgcagagga catgatgcat ccatctggtg ttgccaccga ttacatatgg gagcgctttt
      481 gcaaaacctt tttccggaga gagacccaag acgccatctc agaatggaat cagataagcc
      541 gctctctaaa ccacgtaccg ttgaatgaat caaccgaaaa ctaccgtcaa tttttaaagc
      601 aaacgttaca aaaactgatt ctgttccggc aaaatcaccc ccgaatagat tgtcgccggg
      661 aaacggaaga attaacaaaa aaaataaaac aatgaagtat tccatcctta aaatagcctc
      721 tatactgggc ataaagaatc aaaatattgc cgagggtgaa atatcgctgt tgcttaccga
      781 cagccgaaaa ctaagctctc cgcaggaaac actgttcttc gccatcgaaa ccaaacaaaa
      841 cgatgcgcac aaattcattt ccgatttata cggatcgggc gtaaggaact tcgtggtgac
      901 caagcttttt ccggaatgga aatcctttac ggatgtcaac ttcctgaaag ttaccaacac
      961 gcttcacgca ctgcagaaac tagcggccta tcaccggaaa cagttcagta tccccgtcat
     1021 cgggataacc ggaagtaacg gaaaaaccat tgtcaaagag tggctgtacc agcttctgga
     1081 ggaagactac aacatcaccc gctcaccccg gagctacaac tcccaaaccg gcgttccgtt
     1141 gtccgtctgg gaaatgaccg aaaacaccac cttagccata ttcgaagccg ggatctccca
     1201 acccgatgag atgaagtatc tggaaccggt tatccgcccc acgatcggtg ttttcacgaa
     1261 gctgggggaa gcacatcagg aaaatttctc ctcgcagcaa cagaagtgca tggagaaaat
     1321 ggaacttttc gttaacagca acgtgtttat ttacgaagag gacaacaaga tcattaatca
     1381 atcagccgac aggatggtat tgtcgcaaaa atcgttttgt tggtctcgaa aagaccccga
     1441 tgccccgctt tacatccgta aaacggaaaa aaacggaaac aaaaccacca ttcattactc
     1501 gttcctcaac ttcgagtact ccgtctccat accatttaca gatgaggcat ccattgagaa
     1561 cgccatcagc tgcctggctg ttatgcttta cttaaacgtt aagcccggga caatctcaga
     1621 acgcatgatg aaactggagc ctgtagcgat gcgcctggac gtacgtcaag gaaaaaacaa
     1681 ctgcaccatc atcaacgaca catacaattc agacattaat tccataaaga tcgcgctcga
     1741 tttccagcaa cagcgaaaag tagaacaatc gttgaaaaaa acgctgatta tctccgatat
     1801 cctgcaaacg gggttattgc cgaaatcgct atacaaacat gtagcagaac tggtgcagca
     1861 aagcgggatc gaaaagctta tcgggatcgg acacgatatt tgtgagaaca gtgaggtatt
     1921 cactgtccgg gagaaacact ttttccgctc taccgaagat ttcatccggt ccaatatatg
     1981 gaaagaattc gaaaacgagc tgattctgtt gaaaggcgcc cggagatatc atttcgagca
     2041 gatcaacgag cttctggaac aacggattca cgaaaccgta ctggaggtcg acctggatgc
     2101 cctcgttcat aacttcaact tctataaatc gagaatatcc ccgcaaacga aactcatctg
     2161 catggtgaaa gccaacggat atggagccgg cgccgtggaa attgccaaga ctcttcaata
     2221 tcaccgttgc gcatattttg ccgtcgccgt tgcggaggaa ggaattgagt tgagaaagga
     2281 aggcatttcc actccgataa ttgtgttgaa cccggaagtt aacggatttg aggagttgtt
     2341 ttcgaaagac ctggaacccg aagtgtataa tttcaggata ttggatgcat ttatccggga
     2401 agccgaaaga cgcggcataa ccaactatcc cattcacctg aagattgaca ccggcatgca
     2461 ccggttggga ttccttcccg ctcagatacc ggaactcctg gaaaggttaa aatcacaaaa
     2521 aggattaaag gtgaagtcgg ttttctccca cctcgctgct agtgaaagct ggatgttcga
     2581 cagctttaca caggaacaga tcgatattat tgatgtcgcc caccgggaaa ttggggagaa
     2641 actgggatat cctgtctgga aacacatcct aaattccgca ggcattgaac gatttatcaa
     2701 cgcacaatgg gatatggttc gcctgggaat aggattatac ggagtgagcg caagcggatt
     2761 aaacgggctg aagaacgtct ccacactcaa aactaccatt ctgcagatcc aggagatccc
     2821 aaaccacgag acggtaggct acggaaggaa agaggcccta aaccgcgatg cgaaaatagc
     2881 cactatccga attggctatg ccgacgggct ggacagaaaa tcaggaaaca ggaaaggaaa
     2941 agtatggata aacggcaaat atgcccccat cgtgggaaat gtctgcatgg acctctgtat
     3001 gattgacatt accggcatcg atgcgaagga aggtgattcg gtgatcgtct tcggtgaaaa
     3061 cctgccggta ttggaggtag ccgaaagcat agacaccatc ccttacgaaa tcctgacttc
     3121 catttctccc cgtgtaaagc gtatatacgt aaaagaataa aacaatccaa ttataaacaa
     3181 gatggaacaa ttaagtttaa ctacgccgtc gctgttgttt tcggctatat ccttactgat
     3241 gcttgcctac accaaccggt tcttgtctta tgcacaactg gtgagaaacc tgaaagagaa
     3301 agtgatcgaa gataaaagtt cagtagcgct tccgcaaatt gccaatttac gaaaaaggct
     3361 aaacctcatc cagagcatgc aggtttacgg aataatgagc cttttgttgt gtgtggcaag
     3421 catgttcttc atttatataa aaatgtataa tgcagccggc tacatctttg gcattgcatt
     3481 gatcctgctt atagtttctt tagccatatg cgtacgcgag atacaaattt cgatgcggtc
     3541 gctaaacctg catttacacg acatggaaga aattgaaaaa taaaccagcc ccatacgctc
     3601 ttgggctact ccacaggatc ctgcagcaca aacaatggca tatagcccgc cggcaagcat
     3661 catctttaca tccatttggg acagatcaaa agcaatattc gacttacaac cacaaccggc
     3721 tggtgaacga agtatttgtt tatctgttat ttcgttgttt tcagaaaccg tatagatggt
     3781 gtaaaattca caatgcccga aatgcgcatc gatttgattg ttctcttttg taggtaatgc
     3841 aattttcatg cttcagtttt ttttattcaa ttattgttgt gtatccggtg taaaaccggt
     3901 tcagttgatt tcccaattta ttccggagcc gttcataaac cttttctcca gtacaatgtc
     3961 ctgtaatgaa gctggtctcc ggataattcg ttttaagcgc taaagcaatt gaatctattt
     4021 catccggaga ttcatacagc cgtccttttt cactatcgag cagatgaaag ccgcccatca
     4081 gtgttcccac ttttttcccg gtggacaacc tgacggaatc caggatattc aagagccccc
     4141 tatgtgcgca accgctatag acaaacagtt gctccgaacc aaaagcaaaa atgatctcat
     4201 ggttgaaat
//
LOCUS       DAOSDR010000282         4201 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrimonas sp. isolate AMR_MDS_1826
            NODE_7444_length_4201_cov_4.438013, whole genome shotgun sequence.
ACCESSION   DAOSDR010000282 DAOSDR010000000
VERSION     DAOSDR010000282.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863355
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrimonas sp. (wastewater metagenome)
  ORGANISM  Petrimonas sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Dysgonomonadaceae; Petrimonas.
REFERENCE   1  (bases 1 to 4201)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4201)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:10:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,720
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 35
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4201
                     /organism="Petrimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7444_length_4201_cov_4.438013"
                     /isolate="AMR_MDS_1826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2023866"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..355)
                     /locus_tag="PLI30_10475"
     CDS             complement(<1..355)
                     /locus_tag="PLI30_10475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019538656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HOI80016.1"
                     /translation="MKSKNLFSTKGFFTLLLSVFAFSISAQNVTVSGTVTDDTGLEVI
                     GATVIVAGDASRGTVTDFDGKYTLNNVPSNGSLQISYVGMVTQTIPVNGRTTIDVVLT
                     SDSELLDEVVVTALGI"
     gene            complement(649..1590)
                     /gene="trxB"
                     /locus_tag="PLI30_10480"
     CDS             complement(649..1590)
                     /gene="trxB"
                     /locus_tag="PLI30_10480"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006841829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HOI80017.1"
                     /translation="MRNKVRCLIIGSGPAGYTAAIYASRANLEPVLFEGLQPGGQLTT
                     TTEVENFPGYPEGITGPELMEDLKKQAQRYGTEIFPGRATSVDFSVRPFKVTIDNEHM
                     IEADTVIISTGATAKYLGLPDETKYAGQGVSACATCDGFFYRKKKVAVVGGGDTACEE
                     ATYLSGLADKVYLVVRKPYLRASQIMQERVMNNPKIEILFEHNAVGLYGEKGVEGVHL
                     VKRMGEPDEEKIDLAIDGFFLAIGHTPNTDIFKDFLELDEVGYIKIDNPTSKTNIEGV
                     FAAGDVADPNYRQAITAAGMGCRAAMDAERYLSEKGL"
     gene            complement(1714..2358)
                     /locus_tag="PLI30_10485"
     CDS             complement(1714..2358)
                     /locus_tag="PLI30_10485"
                     /inference="COORDINATES: protein motif:HMM:NF015510.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LolA-like putative outer membrane lipoprotein
                     chaperone"
                     /protein_id="HOI80018.1"
                     /translation="MKKYILAIAALLTVSTGFAQPAANARNILDKAYSTYENSNGIKM
                     TFNITTTDENGTAYQPQKGTARVKGNKFKIEMPTIDTWFDGKTQWVLMKDLNEVNISH
                     PSNEEIASISPLALLSMYKTGFTLDTPASGIINGKNAFVINMSPTGSKSDFKKISVGI
                     DKKTNSVLQVDITLKNGMRNKIDINDYNTNYNFTDTEFIFNKDQHKGVEVVDLR"
     gene            complement(2450..>4201)
                     /locus_tag="PLI30_10490"
     CDS             complement(2450..>4201)
                     /locus_tag="PLI30_10490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005775772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015616 - DNA translocase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HOI80019.1"
                     /translation="TDKPETETEPEVLLQNREQTPLPVTQNTVPPIRKKEPANDVFEV
                     FVPKDEIQVAENKPEEKASPLAESDTNEETDPDVEILEDYDPKKDLSSFAFPTMDLLK
                     VYNTGNRQVDMQEQNENKDKIIRTLRNYGIEITSIKATVGPTITLYEIVPQAGVRISR
                     IRNLEDDIALSLSALGIRIIAPMPGKGTIGIEVPNKEPQIVSMQSVIASVKFQECTYD
                     LPVALGKTITNEVFIFDLAKMPHLLVAGATGQGKSVGLNAIITSLLYKKHPSEMKMVL
                     IDPKMVEFNIYSTIEKHYLAKLPDEEKAIITDVTKVTQTLNSLTKEMDDRYELLMRAH
                     VRAIKEYNEKFIKRKLNPKNGHRYLPYIVVVIDEFGDLIMTAGKEIEMPIARIAQKAR
                     AVGMHMVIATQRPTTNIITGTIKANFPARMAFRVTSQIDSRTILDATGANQLIGRGDM
                     LFSQGSNLIRIQCAFVDTPEIEDISQYIGKQRGYESAFALPEVVSADSEDKPGAVDLN
                     ERDTLFDEAARLIVIHQQGSTSLIQRKFSIGYNRAGRLMDQLETAGIVGPAQGSKPRD
                     VYISDEYSLEKLLDSLR"
BASE COUNT          931 a          953 c          994 g         1323 t
ORIGIN      
        1 tgatccccaa cgccgtcacg actacttcgt ccagtaattc actgtcggat gtgagtacta
       61 cgtcaatggt tgtacgccca ttaaccggaa tggtttgagt gaccattccc acatagctga
      121 tctgcaacga accgttggat ggcacattgt ttaatgtata cttgccgtcg aaatccgtta
      181 cggttccacg acttgcatcg ccagcgacaa ttactgttgc gccgatgact tcaagccccg
      241 tgtcgtccgt tacagtacca cttacggtta cgttttgagc agaaatagaa aaggcaaata
      301 cactcaacag gagtgtaaaa aagccttttg tcgaaaaaag atttttactt ttcattccct
      361 ctagttttta aattgattta ataagttatc cagattttta gcgtgcgttt taaaatattt
      421 ttttgtcttt ttgttgttgt ggcttttgtt tgacaggacg gtttcttgtt ataattgaca
      481 aaaagaagct aaaatatttc aaaatgctcg caaagatagg ttgttttgtt ttattgcaga
      541 aatattttaa caaaaaaata gttgtttaaa attaaacaac tattcactat cgagcctttg
      601 ttcttaaaaa taattaatta gaaaggccgc tttaactgct gaagagaatc acaatccttt
      661 ttccgacaga tacctctcgg catccatggc ggcgcggcat cccattccgg cagccgtaat
      721 ggcctggcgg taattcgggt cggctacatc acctgcggca aaaacacctt cgatgttggt
      781 ttttgatgtt ggattgtcta tcttgatgta tcccacctcg tcgagttcca aaaagtcttt
      841 aaagatatcg gtgttgggtg tgtgtccgat agccaggaaa aatccgtcga tggcgagatc
      901 aattttctct tcatcgggtt cgcccatacg ttttaccaga tgtactcctt ccacgccttt
      961 ttctccgtac agccctacgg cattgtgttc gaaaagaatt tcgatttttg gattgttcat
     1021 taccctctcc tgcatgatct gagatgcgcg caggtaaggc ttacgcacga ccaggtagac
     1081 cttgtctgcc agtcccgaca ggtaagtcgc ttcttcgcaa gcggtatccc caccgcctac
     1141 cacggccacc ttttttttgc ggtagaaaaa accgtcgcat gtggcgcagg cggaaacacc
     1201 ttgtccggcg tatttggttt catcgggcaa ccccaggtac ttggcggttg caccggtaga
     1261 aataattacc gtatcggctt caatcatgtg ctcgttgtcg atcgttacct taaacgggcg
     1321 taccgaaaaa tccacggagg tggcccttcc cggaaaaatc tcagtcccgt atcgctgagc
     1381 ttgtttttta aggtcttcca tcagttccgg cccggtaatt ccttcgggat agccggggaa
     1441 attttccact tcagtagtgg ttgtcagctg cccaccgggc tgtagtcctt caaataagac
     1501 gggctccaga ttggcacgcg aagcatagat agctgctgtg tatcccgcgg gtcccgaccc
     1561 gatgatcagg catcttactt tattcctcat agtcgtaata tgtttaaaaa accttccctt
     1621 tgtttgagag aaggcagtac tgttaataat tggctcaaag gtacgattat tcttctatac
     1681 gtgaaatgga tatagtaaat ttctatcgtc catttatctc aaatccacaa cttcaactcc
     1741 tttgtgttgg tctttgttga agataaattc agtatcggtg aaattataat tggtgttgta
     1801 atcgttgata tcaattttgt ttctcatccc gtttttcagg gtgatatcca cttgtaaaac
     1861 ggagttggtt tttttatcaa tacccacgga tatttttttg aaatcacttt tgcttcccgt
     1921 tggagacatg tttataacga atgcattctt cccattgatg attcccgatg caggggtgtc
     1981 tagcgtgaac ccggttttgt acatgctcag caacgccagt gggctgatgg aggcaatctc
     2041 ttcgtttgat ggatggctga tatttacttc gttgaggtct ttcatcaaaa cccattgggt
     2101 ttttccatcg aaccaggtat cgatggttgg catttcgatt ttaaatttat tgccttttac
     2161 ccgtgccgtc cccttctggg gttggtatgc cgttccgttt tcgtcggtgg tggtaatgtt
     2221 aaatgtcatt ttgatgccgt tggaattttc ataggtgctg tatgctttat ctaagatatt
     2281 ccgggcgttt gcggcaggtt gtgcgaatcc agtagatacg gttaataatg cggcaatggc
     2341 gagtatatat tttttcatat tgttctcctt ttaacgggtt ttctgatggg ttggactaaa
     2401 aagagggtgt ttggtttatt ttgcgatccg ggtttccggt ttccggattt tacctcaagg
     2461 aatccagtaa tttttccagc gaatattcat ccgaaatata tacatcgcga ggcttgcttc
     2521 cttgtgcagg gcctacgatt ccggcggttt ccagttggtc cataagccta cctgcccggt
     2581 tgtacccgat ggaaaatttc cgttggatca gtgacgtgga gccttgctgg tgaattacaa
     2641 taagccgggc ggcttcatcg aagagtgtat cccgctcgtt caggtctact gcgccgggtt
     2701 tatcctcgct atccgccgat accacttcgg gcaaggcaaa tgcggattca taaccccgtt
     2761 gtttacctat gtattgggaa atatcctcta tctcgggcgt atctacaaag gcgcactgga
     2821 tacgaatcag gttgctcccc tgcgagaaaa gcatgtcacc ccgcccaatc aactggttgg
     2881 caccggtagc atcgagaatg gttcttgagt cgatctgtga ggttacacga aaagccatcc
     2941 gggcggggaa gttggccttg atggttccgg taatgatgtt ggttgtgggg cgttgtgtgg
     3001 cgatcaccat gtgcataccc acagcgcgtg ccttttgtgc gatacgtgcg atgggcatct
     3061 caatttcttt tccggcagtc atgatcaagt cgccaaactc atcgatcacc accacgatat
     3121 acggcaggta cctgtgtccg tttttgggat tgagcttccg tttgatgaac ttctcgttgt
     3181 attcctttat ggcacggacg tgggcccgca tcaacagttc gtagcggtcg tccatctctt
     3241 tggtgagcga gttgagtgtt tgggttacct tggttacatc ggtaatgatg gctttctcct
     3301 catcgggaag ctttgccagg taatgttttt caatggtgga gtagatgttg aattccacca
     3361 tttttgggtc gataagcacc atcttcattt ccgacggatg ctttttgtac agcagagatg
     3421 tgatgatcgc attcaagccc accgactttc cctgtccggt tgctccggcg accagcaggt
     3481 gcggcatttt ggccaggtca aaaataaaca cctcgttggt gatggttttt cccagcgcta
     3541 ccggcaaatc atatgtgcat tcctgaaatt tgacggaagc gataaccgat tgcatggata
     3601 caatctgtgg ctctttgttg ggtacttcta taccgattgt ccctttaccg ggcattgggg
     3661 cgataatacg aatacccagc gccgaaaggc tcaaggcaat atcgtcttcc aggtttcgaa
     3721 ttcgtgagat ccggactcct gcctgcggca caatttcgta gagtgtgatc gttggaccga
     3781 cagtcgcttt tatggaggtg atctctatcc cgtagttacg gagggtgcgg ataattttgt
     3841 ctttattctc gttttgctcc tgcatgtcca cctgtctgtt gccggtattg tataccttga
     3901 gcaggtccat ggtagggaat gcaaatgagg acaagtcctt tttagggtcg tagtcttcca
     3961 gaatttccac atccggatcc gtttcctcgt ttgtatccga ctcagccagc ggtgaagcct
     4021 tctcttcggg tttgttttcg gcaacctgta tttcgtcttt cggaacaaaa acctcgaaaa
     4081 cgtcgttcgc cggctctttt tttcggatgg gcgggactgt gttctgcgta acgggaagag
     4141 gtgtttgttc cctgttttgc agcaaaactt ccggttcggt ctctgtttcc ggcttgtccg
     4201 t
//
LOCUS       DAOSDR010000283         4200 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrimonas sp. isolate AMR_MDS_1826
            NODE_7447_length_4200_cov_4.233776, whole genome shotgun sequence.
ACCESSION   DAOSDR010000283 DAOSDR010000000
VERSION     DAOSDR010000283.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863355
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrimonas sp. (wastewater metagenome)
  ORGANISM  Petrimonas sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Dysgonomonadaceae; Petrimonas.
REFERENCE   1  (bases 1 to 4200)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4200)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:10:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,720
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 35
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4200
                     /organism="Petrimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7447_length_4200_cov_4.233776"
                     /isolate="AMR_MDS_1826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2023866"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            578..1996
                     /locus_tag="PLI30_10495"
     CDS             578..1996
                     /locus_tag="PLI30_10495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019540488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOI80020.1"
                     /translation="MKENRRHFLKQITAGGIGLSFIPGLRAEKSISEPYEILSKETGR
                     RQYFNMCGYRAPKLDIVRVGFIGIGNRGYANLNQMTFLEGVHIKAICDIVQFRIDNVQ
                     QLLRKRGLPEAEVHTGREDAWRKLCEDPNIDLVSIAVPRGPLHARISVYAMECGKHVA
                     VEVPAIATVEEAWEVIEASEKTRKHCYMMENCCYDFFELLTLNMVRQGFFGDIIHADA
                     AYIHHQSLYEKTKDNNMWRLEESQRHNGNLYPTHGLGPVCQVMDINRGDKLDYMTSMS
                     SNDFMLGKKVSELAQSDPFYKKYDTNSYRGNMNTSIIRTEKGRTIMLQHDITSPRVYS
                     RIHMISGTKGAVQKYPEPGRISLGSEWLDNEKMNEINDKYKPEIVKRIGDMAQKVGGH
                     GGMDFLMTWRLIDCLRNGLPMDIDVYDAASWSVIIPLSEKSVSNRSKPVEIPDFTRGA
                     WRTNQPVDISISEGNTTTIRRS"
     gene            complement(2465..3046)
                     /gene="pnuC"
                     /locus_tag="PLI30_10500"
     CDS             complement(2465..3046)
                     /gene="pnuC"
                     /locus_tag="PLI30_10500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005778616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015663 - nicotinamide mononucleotide
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015890 - nicotinamide mononucleotide
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HOI80021.1"
                     /translation="MQTLEIIGAVIGLIYLYLEYKANRWLWLFGVIMPVVYIVIFYQS
                     KFYADMGINIYYFFASIWGWMVWTKKKKNSAKNGKISHTPTRFIVPLTLAGIAIFALI
                     AFILVNYTDSPVPYGDAFTTALSIIAMWMLAFKYIEQWGLWFVVNSISCGLYFWKDLH
                     YTSGLFAIYAVISIFGYFKWKRMMKDEFRPFTV"
     gene            complement(3125..>4200)
                     /locus_tag="PLI30_10505"
     CDS             complement(3125..>4200)
                     /locus_tag="PLI30_10505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019537873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOI80022.1"
                     /translation="NIPEDYEWYRNVGKKGEFNFFTKAEYRLNEKVSLFGDLQYRHID
                     YDFSGIDDDLADITSHFKYSFFNPKAGASYRIDNRNNLYASVAVGQREPLRTDLKESI
                     KGGGGEERIRPEKMIDYELGYRFSHENGVSLGANIYYMDYKNQMVQTGKLNDVGYKLM
                     ENVKDSYRTGVELEASVPLANDKFRFDANATLSRNKINNYTAYFDLYDNSNDWNEVYI
                     EKNGKQVRKQTRKELGTTHISYSPNLVGMASLTYQPLPALYFNLLGKYVSKQYLDNTS
                     DNAKSIDGYFVSNFSAGYTFKKTPLGSIALQLFVNNLFNKEYIANGWASTEVFEEDGS
                     SVNWIGYYPQATRNYMARLTVSF"
BASE COUNT         1222 a          821 c          934 g         1223 t
ORIGIN      
        1 ttatttataa ctttttcccg ctaaattctt taggaataac tgcggataat catatttatt
       61 tgatttctga tttttaatat cttctctcct ctgcctgaat aaacatccgc aggcgtaagg
      121 ttgtcaagcg attcgggata acgttcatac cttgtttttg cataaagtta gtttgttata
      181 aaattagttg tttttgtgaa attactaaac taaatttgca cttaaccaac cggtaaactt
      241 tatactgaag atttacagtt tggagcgatt aacggaagat ttctgtttca ggtagattga
      301 tgtttgtgca agattgggaa taagccattt tcaactcgat tggggttggc aggaatcaga
      361 taaatggata cttaaccgct cgcctttatg gatgtctctg atttaccgga agatactttt
      421 gattcatccg atatcattaa aaaatacaaa agcatccggc ataatttcat aaataaaaga
      481 aaattgggct tttttaaggt tagacttatt aaaatggttt tggactaaat tgggctaatt
      541 gagaaataat atttctattt acaaataaag taatcgaatg aaagaaaacc gacgtcattt
      601 tttaaaacag ataacagcag gaggtattgg cttaagcttt attcctgggc ttcgagctga
      661 aaaatctatt tcagaaccat atgaaattct ttccaaggaa actggaagaa gacagtattt
      721 taatatgtgt ggttaccggg ctcccaagct ggatatcgtt cgtgtaggat ttatcgggat
      781 agggaatcgt ggttacgcaa acctgaacca gatgaccttt ttggaaggag ttcatataaa
      841 ggcaatttgt gatattgtac aatttcgcat tgacaatgtt caacagttgc tgagaaaacg
      901 gggattgccg gaagcagagg tccataccgg acgtgaagat gcatggagaa agctatgtga
      961 agacccaaac atcgacttgg taagtattgc tgtgccacgg ggaccgctcc atgcacgaat
     1021 atctgtgtat gctatggagt gtggtaagca tgtggctgtt gaagtacctg caatagctac
     1081 cgttgaagag gcatgggaag tgattgaggc ttcggagaag accagaaagc attgttatat
     1141 gatggaaaac tgttgttatg attttttcga gctactaacg ttaaatatgg tgcgacaagg
     1201 atttttcgga gatattattc atgccgatgc tgcatatatt catcaccagt cactttatga
     1261 aaagacaaaa gataacaata tgtggcgttt ggaagaaagt cagcgccata atggaaattt
     1321 gtatcctacg catggattag gacccgtatg ccaggttatg gacataaaca gaggagacaa
     1381 actggactac atgacctcaa tgtcttcaaa tgattttatg ttgggaaaga aagtttcgga
     1441 actggcacaa agtgatccgt tttataaaaa gtatgatact aactcctata gaggaaatat
     1501 gaatacttca ataatcagga cggagaaagg gcgtaccatc atgcttcaac acgacattac
     1561 cagtcctcgg gtctactccc gaatacacat gattagcggc acaaaaggtg ctgttcagaa
     1621 atatcctgaa cccggtagga tttcacttgg cagtgaatgg cttgataatg aaaagatgaa
     1681 tgaaattaac gacaaatata agccggagat tgtaaaacga ataggtgata tggctcaaaa
     1741 agtaggagga cacgggggaa tggatttttt aatgacatgg cggcttattg attgtttgag
     1801 aaacgggctt ccgatggata tcgacgtgta tgatgctgct tcttggagcg tgattatccc
     1861 gttaagtgaa aaatcggtat cgaatcgttc aaaacccgtt gaaattcctg attttacacg
     1921 aggagcctgg agaacaaatc aacctgtaga tatctccata tcggaaggga acacaacaac
     1981 gataaggcgc tcttaagtag agtgtcgata taatatcccg ggtagtaatc cagaaaactg
     2041 tgttttttat ataacttcac gaccatagtt ttttaattat gtgctttttc gctaataatc
     2101 aaactaataa gtgaagtcct gggctgattt ctaaattatg aaaatcgaga ctaagcttga
     2161 caagttttta acaaattaac taacttgtaa tctcaaatct tgatttttat gactttaaaa
     2221 gcatcatcag acatcagacg caaactacgt gttcttactt atgaatatgg agtctatcga
     2281 ccttcccaaa acagcataat gaactcctat tctgacgaat ttaacgctcc ctccagactt
     2341 gctatctaca aaagaattat ggagctgtca ggagaaggtt acagctacgg caaatttata
     2401 gagtctgatg caattaacag gagtgcaggt acccgaagtg aaccttaggc gcctccggtt
     2461 gtagttagac ggtaaaaggg cgaaattcat ccttcatcat ccgcttccat ttgaaatatc
     2521 caaaaatgga aataacggca tagatggcaa acaatccgct ggtgtagtgt aggtctttcc
     2581 agaagtaaag gccgcaggaa atactgttca ccacaaacca caatccccac tgttcgatgt
     2641 atttgaaagc caacatccac atggcaatta tgctcagggc tgtagtgaat gcatctccat
     2701 acggtaccgg gctgtctgtg tagttgacca ggataaaggc tataagtgca aaaatagcaa
     2761 tacccgccaa cgttaacggt acaatgaaac gggtgggagt atgggagatc ttcccgtttt
     2821 tcgctgaatt cttcttcttc tttgtccaga ccatccatcc ccagatactt gcaaagaaat
     2881 agtagatgtt tatgcccatg tcggcataaa acttggattg gtagaagatg acaatataca
     2941 ccaccggcat aataacgccg aagagccaca accagcggtt ggctttgtat tcgagataga
     3001 ggtagataag cccgataacg gctccaataa tttcaagggt ctgcatatga attgcgggtt
     3061 atattttgca ggccgggccg ggtgagccgc agcccgcaaa aacttctttt caggcgaaca
     3121 gggtttagaa actaaccgtt agacgtgcca tataattccg ggtggcttgc ggatagtagc
     3181 caatccagtt tacgctgcta ccatcctctt caaatacttc ggtagaagcc cacccgttgg
     3241 caatgtattc tttattgaac aggttgttca cgaagagctg cagtgctata ctgcccaacg
     3301 gtgttttctt gaatgtgtat ccggccgaga agttgcttac aaaatagccg tcaatcgatt
     3361 ttgcattgtc cgatgtgttg tcgagatact gtttgctcac atatttcccc aacaaattga
     3421 aatagagcgc cggaagaggc tgataggtca ggctagccat acccaccagg ttgggagaat
     3481 aggaaatgtg ggttgtcccc aactctttac gggtctgttt gcgtacctgc ttgccgtttt
     3541 tttctatata aacttcgttc cagtcattgc tgttgtcgta cagatcgaaa taggccgtgt
     3601 aattgttgat tttgttccgg ctcaatgtgg cgttggcatc aaaacgaaat ttgtcgtttg
     3661 ccaacggtac cgatgcttcc agttccacac ctgtccggta actgtctttc acgttctcca
     3721 tcaacttgta tcccacgtcg ttgagctttc cggtttgcac catctggttt ttgtaatcca
     3781 tgtagtaaat gtttgcaccc aggctcacac cgttttcgtg ggagaaacga tatcccaact
     3841 catagtcgat cattttttcg ggacggatcc gctcttctcc tccgcctccc ttgatgcttt
     3901 ccttcaggtc ggtccgaagc ggttcgcgct gcccaacagc caccgaggca tacaggttgt
     3961 tgcggttatc tatccggtag gaggctccgg ctttgggatt aaagaaggag tatttgaagt
     4021 ggctggtgat atccgccagg tcgtcatcaa ttcctgaaaa atcataatcg atatgcctgt
     4081 actggagatc gccgaagagc gaaacttttt cgttgagccg gtattccgct ttcgtgaaga
     4141 agttgaattc acccttcttc cctacatttc ggtaccattc gtaatcttcg gggatgtttt
//
LOCUS       DAOSDR010000284         4195 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrimonas sp. isolate AMR_MDS_1826
            NODE_7462_length_4195_cov_4.087923, whole genome shotgun sequence.
ACCESSION   DAOSDR010000284 DAOSDR010000000
VERSION     DAOSDR010000284.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863355
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrimonas sp. (wastewater metagenome)
  ORGANISM  Petrimonas sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Dysgonomonadaceae; Petrimonas.
REFERENCE   1  (bases 1 to 4195)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4195)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:10:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,720
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 35
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4195
                     /organism="Petrimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7462_length_4195_cov_4.087923"
                     /isolate="AMR_MDS_1826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2023866"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..459
                     /locus_tag="PLI30_10510"
     CDS             <1..459
                     /locus_tag="PLI30_10510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI80023.1"
                     /translation="KMIADRGGADICYSGPGWTGHLAFIEPDAPEFRGSLEDWKQMGA
                     RVCTLSPFTLAQNSLHGVFGKSGNLAAVPPKAATIGPADVINAKHRFDTHAITVHGTT
                     TAWQRFTTRLVLHGPVTPLVPESILQTLRTDVYVSETIAQNIETDWNKGY"
     gene            471..1787
                     /locus_tag="PLI30_10515"
     CDS             471..1787
                     /locus_tag="PLI30_10515"
                     /inference="COORDINATES: protein motif:HMM:NF016612.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="neutral/alkaline non-lysosomal ceramidase
                     N-terminal domain-containing protein"
                     /protein_id="HOI80024.1"
                     /translation="MTELKAGAATVNITPQESHFLFGYPFVDRMSTGTHDDLLSSALY
                     LTDGKNQVLFISNDVIYVSKASTARIREGISKETGIQASNILVAATHTHSAPVTVDVV
                     ISANDPIVPKVDRNYLKFMEESTVKAACQAVENATLAEIAVVVGDATGVGTNRHNPEW
                     AKDTDVPAVFVKNKYNDEFISCMLICNMHPTILHENSTLYSSDFPHFVRKTLQEVVLG
                     NDRPVIYFTGTAGNQSPRHVTKSNTFEEAKRIGQIVADSISSKLTETVTFSSHIPVSA
                     AQKFVDLPKRAFPSIEWAVEHRDKTKKRFEELKKNSEIPQEVRTAEVNWFGSEELLYL
                     SKLAQDNKLRKAYQSSLPAEIQIIKVGEWKFVAWPGEVFVEYGIELKNHAKEIALITY
                     ANGELQGYLVTQEAQDGGYYEAGNSFFDYKAGKLMLAETIKELKSM"
     gene            1789..3204
                     /locus_tag="PLI30_10520"
     CDS             1789..3204
                     /locus_tag="PLI30_10520"
                     /inference="COORDINATES: protein motif:HMM:NF012589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HOI80025.1"
                     /translation="MGFYLGIDLGTSYFKAGIFDEQGNLAGLGRCLVIKQTDDGKICE
                     LPIADFWVTLRKCINRAIEKAEINPNELIAVSYSSQANSFILLDETDHPLTPLILWPD
                     KRAGQISSVLDSLLENPNFLKKTGLGILPGLQSMAAKIVWFQENKPEIWEKVNSIASV
                     SDYLTFSLTGQKVSDFSTSSMTGLFDLLEEKWWNEALETLNINKKFLFSPKRTGSFVG
                     TLAGERAIQLGLSADTKFFLGGLDHHIVAVGAGLTSSTQISESTGTVLACVNYHQGFS
                     PRAGINIAPGLRKEYYFQMAFNENGATALEWYQKKFAPELSIEELLKMAEGIAPGSDG
                     LKASPCVDKFESLSGFQNITNKHSHGHFVRAILESTGLSLRDLICSLDKENVSEVLIA
                     SGGGARSLLWMQIKADILNKVFMVPECSELACQGAALIGAIGSIQQEDREKYFEKQAR
                     YAQLINPNPADVEKYKLENKL"
     gene            3201..3806
                     /locus_tag="PLI30_10525"
     CDS             3201..3806
                     /locus_tag="PLI30_10525"
                     /inference="COORDINATES: protein motif:HMM:NF013539.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HOI80026.1"
                     /translation="MKELTEDAILQRAIQSLRIESKAISDIIDYLDKESFLKAVEVLS
                     SCPKIITCASGSSGIAAKKFAHSLCCIERNAQFLSPAEAIHGGMGCMKKGDAVVMVSR
                     GGKTAELLPIIEVCNKKEVILIGVTENLDAPLAKNSQIVVPMKIEKESDGLNVMATAS
                     FVATIAIFDAMLASIMETTGYSLEQFALIHPGGAVGSRLNG"
     gene            3846..>4195
                     /locus_tag="PLI30_10530"
     CDS             3846..>4195
                     /locus_tag="PLI30_10530"
                     /inference="COORDINATES: protein motif:HMM:NF012349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HOI80027.1"
                     /translation="MYKGLTIKPPFFEIGPKAYMYGERMLRLAKAADEASVKYDVRII
                     LTLQPSDIYLLARETEHVLIFAQHMDPVPIGRGNGSILPEALKAAGAVGVILNHAEKP
                     MTLADLSQAIKRAD"
BASE COUNT         1300 a          890 c          999 g         1006 t
ORIGIN      
        1 aaaatgattg ctgaccgtgg tggggccgac atatgctaca gtgggcccgg gtggacaggc
       61 catttagcct ttatcgaacc ggatgcgccc gaattccggg gttccctgga agactggaaa
      121 cagatgggag caagggtctg cacgttaagt cctttcactc tcgctcaaaa ctcattgcac
      181 ggtgtttttg gcaagagcgg aaatttggcg gcagtgcctc caaaggctgc gacaataggc
      241 cccgctgatg taataaatgc caaacaccgc tttgacacac acgctattac tgtacacggt
      301 acaaccaccg catggcaacg atttactacg cgtctcgtac tgcacggacc ggtaacgccg
      361 cttgtgcccg aatccatcct tcagacattg aggaccgacg tatacgtatc ggaaaccatc
      421 gcacaaaaca tagaaacaga ctggaacaaa ggatattaaa aaaaccaaag atgactgaat
      481 taaaagccgg cgcagcaacc gtaaatatta ccccgcaaga atcacatttc ttgttcggtt
      541 acccgtttgt tgatagaatg agtaccggca cacacgatga tttgttaagt tcagctttgt
      601 atttaacgga tgggaagaat caggttttgt ttatttccaa cgacgttata tacgtgagta
      661 aagcatctac agctcggata agagaaggaa tttctaaaga aaccgggata caggcttcca
      721 atatactggt agcggcaaca catacacatt cagctccagt tactgtagat gtggtaatca
      781 gtgcgaacga cccaatcgtt ccaaaagttg atagaaacta cctgaaattc atggaggaga
      841 gcaccgtaaa agcagcttgc caggcagttg agaatgcaac gttggctgag attgccgttg
      901 ttgttggaga cgcaaccggt gtgggaacca atcgacacaa tcctgagtgg gcaaaagaca
      961 cggatgttcc ggcagtattt gtcaaaaata agtacaatga tgaatttatc agttgtatgc
     1021 tgatttgcaa catgcaccct accatattgc acgaaaactc aacactgtac agcagtgatt
     1081 ttccgcattt tgtccgaaaa acccttcagg aagtagtttt aggaaatgac cgtccggtca
     1141 tctattttac cgggactgcc gggaatcaaa gcccgagaca cgtgacaaaa tccaacacat
     1201 ttgaagaggc caaacgaata ggacagattg ttgcagattc catcagctcc aagctaacgg
     1261 agacggtgac tttctcctcc catatacctg tttcagccgc tcaaaaattc gtcgatttgc
     1321 ccaaacgggc ttttccctcg attgaatggg cagttgaaca tcgcgacaaa accaaaaaac
     1381 gtttcgaaga gcttaaaaag aattcagaaa ttccgcagga ggtccgcacg gcagaagtga
     1441 actggtttgg ttcagaagaa ctcctgtatt tgtcgaaatt agctcaggac aacaaattga
     1501 gaaaagcata ccaaagctct ctacctgctg aaatacaaat aattaaagtg ggagaatgga
     1561 aatttgtagc ctggccaggg gaggtgtttg tagagtacgg cattgaactg aaaaatcatg
     1621 ctaaagagat cgctttgata acctatgcca atggcgaact tcaaggttat ctcgttaccc
     1681 aggaagccca ggacggaggt tattatgaag ctggaaattc tttcttcgat tacaaggccg
     1741 gtaaattaat gctggccgaa acaatcaagg aattaaaatc gatgtgaaat gggattctat
     1801 ctcggaatag atttaggaac aagttacttc aaggcgggca tattcgatga acaaggaaat
     1861 ctcgccggac tgggacgttg tcttgttatt aaacaaacag acgatggaaa gatctgtgaa
     1921 ttgccaatcg ctgatttttg ggttacacta cgtaaatgca ttaatcgggc aattgaaaag
     1981 gcagaaatca acccaaacga gctaattgct gtttcctatt catcgcaagc caacagcttt
     2041 atcctgttag atgaaacgga tcatccgtta actccattga ttctgtggcc ggacaaaaga
     2101 gccggacaaa tttcttccgt tctggactct ttattggaaa accctaattt tctaaagaaa
     2161 acaggattag gaattttgcc cggtttgcaa tcaatggcag ctaaaatagt ttggtttcaa
     2221 gagaacaagc cggaaatctg ggagaaggta aacagcattg cttccgtttc agactacctg
     2281 acattctcgt taaccggaca aaaagtaagc gacttcagta catcatccat gacaggactg
     2341 ttcgatctac ttgaagaaaa gtggtggaac gaagcattag aaacactgaa tatcaacaaa
     2401 aaattcttgt tttctcccaa aagaacagga agttttgtcg gcactttagc cggagaaaga
     2461 gctatacaat tgggattgtc tgccgacacg aaattcttcc tgggaggatt agaccaccat
     2521 attgttgctg taggtgctgg cttaacgagt tcaacccaaa tttccgaatc aacaggcact
     2581 gtgctggcat gcgtgaatta tcatcaagga ttctctccga gagcgggaat aaacatagct
     2641 ccaggcttaa gaaaagaata ttattttcaa atggctttca atgaaaatgg agctacagca
     2701 ctagagtggt atcaaaaaaa gtttgctcct gaattatcca ttgaagaact gttaaagatg
     2761 gccgaaggca ttgccccggg aagtgatggt ttaaaagctt ctccctgtgt tgataagttt
     2821 gaatcacttt cgggattcca gaacattaca aacaagcatt cccacggaca ttttgtaagg
     2881 gcgattctcg aatcgactgg tttaagttta cgagacttga tttgcagtct ggataaagaa
     2941 aacgtatcag aggtgttaat agcttcagga ggaggagccc ggagcctttt gtggatgcaa
     3001 ataaaggccg atatcctcaa taaagttttt atggtacccg aatgcagtga attggcctgc
     3061 cagggagccg cattgatcgg agcgataggg agtatccaac aggaagacag ggaaaaatat
     3121 ttcgaaaaac aggcaagata tgctcagctg ataaacccaa atccagcgga tgtagaaaaa
     3181 tataaattgg aaaataaatt atgaaagaat taacagaaga tgcgatcctg caacgcgcaa
     3241 tacagtcctt aaggattgaa agcaaggcca tttcggatat cattgattac ctggacaaag
     3301 agagcttttt aaaagccgtt gaagtattga gcagctgtcc caagataata acctgcgcca
     3361 gcgggtcgtc cggtatcgct gcaaaaaagt tcgcacactc gttgtgctgc atcgagagaa
     3421 acgcacaatt cctgtcgccg gcggaagcca tccacggagg aatgggatgc atgaaaaagg
     3481 gagatgcggt agtgatggtc tcccggggag gaaagaccgc cgagctgtta cccatcatcg
     3541 aggtgtgcaa taaaaaagag gtgatcctta tcggggttac cgagaacctg gatgctcccc
     3601 tggctaaaaa ttcacaaatc gttgtcccca tgaaaatcga gaaggagagt gacgggttga
     3661 atgtcatggc aacggccagc ttcgtagcca cgattgccat ttttgacgcc atgcttgcct
     3721 ccattatgga aaccaccggg tattcgttgg agcagtttgc cttaatccac ccgggcggcg
     3781 ctgtcggcag caggcttaac gggtaaatag agtatctgaa accaaaaata aaattaccaa
     3841 ataatatgta taagggatta acaataaaac cgccattttt tgaaattgga cctaaagctt
     3901 acatgtatgg ggaaaggatg ctgaggctgg caaaagcggc cgatgaagct tccgtcaagt
     3961 acgatgtgag aataattcta acactgcaac cgtcggatat ttacctgctg gccagggaga
     4021 cggaacacgt gctcattttt gctcaacaca tggatccggt cccgataggc aggggaaacg
     4081 gctccatcct tccagaggcg ctaaaagcag ccggcgctgt cggtgtcatt ctcaaccacg
     4141 ccgaaaaacc gatgaccctg gctgatttgt cccaggcaat taaacgggcg gatga
//
LOCUS       DAOSDR010000285         4185 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrimonas sp. isolate AMR_MDS_1826
            NODE_7493_length_4185_cov_4.221065, whole genome shotgun sequence.
ACCESSION   DAOSDR010000285 DAOSDR010000000
VERSION     DAOSDR010000285.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863355
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrimonas sp. (wastewater metagenome)
  ORGANISM  Petrimonas sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Dysgonomonadaceae; Petrimonas.
REFERENCE   1  (bases 1 to 4185)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4185)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:10:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,720
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 35
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4185
                     /organism="Petrimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7493_length_4185_cov_4.221065"
                     /isolate="AMR_MDS_1826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2023866"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(116..895)
                     /locus_tag="PLI30_10535"
     CDS             complement(116..895)
                     /locus_tag="PLI30_10535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI80028.1"
                     /translation="MKNKLYIGLAWATIFFASSSCSEDYIIGGEVNETKQLNMTTFDL
                     LKSMEETKNVAILFERAGMKELVNGDVTIISPSQWSINRYLRRKHNQNLRNNPSAPPV
                     TMDDISAQELQKMGMYILPGKYWRETIPAEGINLVAHDGTEVRLTLDKTNTDPGAAWD
                     GSGVPGSGYQYSNFMQQVPYLVKVHFKRGANWEMTPDDRNRLTGYYDNPECDHVYRMY
                     VSDILTSTGVVHVIYSGDYGYSDHYYYHSLFFFGLRTDDKL"
     gene            complement(922..2538)
                     /locus_tag="PLI30_10540"
     CDS             complement(922..2538)
                     /locus_tag="PLI30_10540"
                     /inference="COORDINATES: protein motif:HMM:NF019592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HOI80029.1"
                     /translation="MKRIYIFLLLFPFLLNGCTDFLEPDQVNLVYNEVFWKNQNDAER
                     ALNGVYALYRGVMVDGNWYERGDATTGFFNRGWNGGSSDALYKPGDFSSASSTQRSWG
                     NLEGLSDWSDFYKVIAHANLVIKKIEGMSDDLFQEGKKQQFLGEAYFMRGLVYFNILR
                     IWGNAPYISDAIESSTQVINDDLTPVLIGRTKDTDLAANILEDIEKAIQMLDYQRPGT
                     ADWGIRASRASAEALSGHANLWMHFLATRDNLGNPGQYLTKAITSLESLKNKGGYILE
                     SYEGADALTRLYKGQSSEAIFELNISSEQNESYRIDHGGVVRLTCKVVSLDGDATKDR
                     SSSVNFVPRSQKELIYPDYDFDQRTGDRRANLFFDVWYSHYNEPFSDVSSTSTDRNLV
                     TWMKKYSMMTIDASRAWNEYSAYFAEANIPVFRYTDAILLLAEAYAKSGQPGKAVDIV
                     NTIRERAGLTPYSGDNLLNEVLQQRISELIGEGQIYFDMVRNNYFPNPQVMDQARYQQ
                     QGYYWPVSGNILVSNKMVSQTPYWNGKTSW"
     gene            complement(2551..>4185)
                     /locus_tag="PLI30_10545"
     CDS             complement(2551..>4185)
                     /locus_tag="PLI30_10545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI80030.1"
                     /translation="TFLAGTELNSDVQQMSWITADNGTSDYLKVIQGFQKVDINGYSN
                     IVTTNMLSFFGSASYGFKENKYQVEGVIRRDASSRFGANNKWATFPSVKVHWVFSKEP
                     WMEAQSDWLDFGKIRVSYGTSGSIAGDPLLQYNSLISTNNVGAGMNNIYANKMDVKTY
                     GGQTILISDFNKVSNKSLSWSKSEEINYGIDLELLGKRLFVTGDIYSKYISGLVYTSY
                     LPPYLGFSSIQSNLVDMISNGYEMSFTGYLFPRTSDFQWEWTANFSRNKTIIANLGNG
                     GRDYIDGDYAFVVGRPAFQYYTYEYIGALDSYDDLPVNPATGEALKYLWADAGLALGL
                     QGKIFPGMPLFTDVNGDYMIDGGDYGYDKKIIENKSPEPDIMGGLHTTLRYKGLSLRV
                     QSSFAFGHHIFNTTLQEQLSTYDDNVAFFTKALYKVDSDKFWKESGDNSYYPMRYVSY
                     SDGGSVRAFRRSSMFLEKGDYWSLDNITLSYNLPKKLLSSFKIRGINMYATARNVFLW
                     KKSGVLDPRAVSKTGYYNGNGYPISRSVIMGFQFQF"
BASE COUNT         1121 a          986 c          900 g         1178 t
ORIGIN      
        1 ggtatatatt atcactcaaa aaacctgcac cctctggctc cttaaagcag gaaacgagta
       61 atatcgccaa taaaacaaga gttatattct taatcatagc aaaataattt taaggttata
      121 gtttatcatc cgttcttaat ccgaagaaga atagtgaatg gtaataatag tggtcggaat
      181 agccgtaatc gccggagtag atgacatgta ccactccggt tgaggtgaga atatccgaaa
      241 catacatgcg gtaaacatga tcacactcag gattgtcgta ataacctgtc aaccggttac
      301 ggtcatctgg ggtcatctcc cagttggccc ctcttttaaa atgaacttta accaggtaag
      361 gcacctgctg catgaagttg gaatactggt atccgcttcc cggtactccg gaaccatccc
      421 aggcggctcc cggatccgtg ttggttttat caagggtaag gcgaacctcc gttccgtcat
      481 gggcaaccag gttgatgcct tcggctggaa ttgtttcacg ccagtatttg ccaggcaaaa
      541 tgtacattcc cattttctga agctcctgag ccgaaatgtc atccatcgtt acaggtggcg
      601 cagaaggatt gtttctgaga ttctggttgt gtttgcggcg caggtagcgg ttgatagacc
      661 actggctggg agaaatgatg gtgacgtcgc cattcacaag ctccttcatg cctgcccgtt
      721 caaaaagtat tgccacattt ttggtctctt ccatcgattt tagcaggtcg aaggtggtca
      781 tattaagttg tttggtttcg ttcacctctc caccaataat ataatcctcc gaacaagaac
      841 tacttgcaaa aaatatagta gcccaagcaa gacctatata taatttgttt ttcataacat
      901 tcaatttttt atttcgatta actaccatga agttttacca ttccaataag gggtctgtga
      961 taccattttg tttgatacca gaatgttgcc agacaccggc cagtaatatc cctgctgctg
     1021 atagcgcgcc tggtccatta cctgcggatt cgggaaatag ttgttgcgaa ccatatcaaa
     1081 atagatctgg ccttcaccaa tcaattcact gatcctttgc tgcagtactt cattaagaag
     1141 attgtcgccg gagtagggag tcaggcctgc ccgctctctg atcgtattca caatatcaac
     1201 tgcttttcct ggttgccccg actttgcata ggcctcggcc agcaggagaa tagcatccgt
     1261 gtatctgaaa accgggatat tggcttcggc aaagtaagcg ctgtactcat tccaggcccg
     1321 cgaagcatca attgtcatca tcgaatactt tttcatccac gttaccagat tacggtcggt
     1381 ggaggtcgaa cttacgtcgc taaacggctc attgtaatgt gaataccaga catcgaaaaa
     1441 caggtttgcc cgccggtctc ccgttctctg atcaaaatcg taatcgggat agatcagctc
     1501 tttttgtgac ctgggcacga aatttacgct ggaggatctg tctttggtgg catcaccgtc
     1561 gagtgaaaca accttgcagg ttaaacgaac taccccgcca tgatcaattc tgtacgactc
     1621 attctgttcg gatgaaatat tcaattcgaa aattgcttcc gaagattgtc ctttgtacaa
     1681 acgagtaagg gcatcagctc cttcgtaact ctccaggata tacccgccct tatttttcaa
     1741 ggattcgagc gaagtgatcg ctttggtcag atattgacct ggattgccca gattatcccg
     1801 ggtagccaaa aagtgcatcc acaggttggc gtgtccggac agtgcttctg ctgaagcacg
     1861 gctggccctg attccccaat cagcggttcc gggccgctgg tagtcaagca tctggatggc
     1921 tttttcgata tcttccagaa tgttagcagc caaatcggta tccttggtcc tcccgatcaa
     1981 taccggtgtt aagtcgtcat tgataacctg ggttgaagat tcgatggcat cactgatgta
     2041 gggagcgttt ccccagattc gcagaatatt gaaataaacc aaccctctca tgaaataggc
     2101 ttctccgagg aattgctgtt ttttaccctc ctggaacaga tcatcggaca ttccttcgat
     2161 ctttttgatc accagattcg cgtgggcaat gaccttgtaa aaatcggacc aatcagacag
     2221 cccttccagg ttaccccagg atctttgggt ggatgaagca ctgctgaaat ctcccggttt
     2281 gtataacgca tcgctggagc cgccattcca tcccctgttg aaaaaaccgg tagtggcatc
     2341 acctctttca taccagtttc cgtcaaccat caccccgcga tataaagcat aaaccccgtt
     2401 aagcgcacgt tctgcatcat tctgattttt ccagaaaacc tcattgtaaa ccaggtttac
     2461 ctgatccggc tccagaaaat cggtacatcc attcaataaa aatggaaata ataaaaggaa
     2521 aatatatatt cttttcatgt tgcttaattt ttaaaattga aactgaaatc ccataattac
     2581 actacgtgag atcggataac catttccgtt atagtaacct gttttggaca cggcgcgtgg
     2641 atccaatact ccggactttt tccacaagaa cacattccgg gcagttgcat acatgtttat
     2701 tcccctgatt ttgaaagagg ataataattt ctttggaaga ttataggata acgtaatgtt
     2761 gtccaggctc cagtagtcac ctttttcaag gaacatggat gatctgcgga acgccctgac
     2821 tgaaccacca tcggaataac ttacgtatcg cataggataa taggaattat ctcctgattc
     2881 tttccagaac ttgtcactgt cgaccttgta caatgctttg gtgaaaaagg ccacgttatc
     2941 atcgtaagta gacaactgtt cctgcagggt ggtattaaag atatgatgtc cgaaggcaaa
     3001 agaagactga acccttaacg acaatccttt atacctcagt gtggtgtgaa gtcctcccat
     3061 gatatctggc tccggagatt tgttttcgat aatcttctta tcatatccat agtcacctcc
     3121 atcaatcatg tagtcaccat ttacatccgt aaacagcggc atgccgggga agattttccc
     3181 ttgaagaccg agcgccaaac cggcatctgc ccataaatat ttcaaggctt caccggtcgc
     3241 cggatttacc gggagatcat cataggaatc gagggcgcca atatattcgt aggtgtaata
     3301 ctggaacgct ggccttccta ccacaaaggc gtaatcgccg tcgatgtaat cacgtccccc
     3361 atttccaaga tttgcaataa tggttttgtt tctggagaag ttagctgtcc attcccactg
     3421 gaaatcactg gtccgcggga ataaatagcc ggtaaagctc atttcataac cattgctgat
     3481 catatccaca agatttgact gaatggaaga gaaccccagg tagggaggca ggtaggaagt
     3541 gtaaaccagg ccggagatgt atttggagta gatgtctccg gttacgaaca ggcgtttccc
     3601 aagcagttca aggtcaatac cgtagttgat ctcttctgat ttgctccacg agaggctctt
     3661 gttggacacc ttgttaaagt cggaaatcaa aattgtttgg cctccgtaag ttttgacatc
     3721 cattttgttg gcataaatat tattcatgcc tgcccccaca ttgttggtgc tgatcagaga
     3781 gttatactga agcagcggat cgccggcaat agaaccagag gtaccgtaag aaacgcggat
     3841 tttcccgaag tcgagccaat ctgactgagc ctccatccag ggttctttcg agaataccca
     3901 atgaactttt accgaaggga aggttgccca tttgttgttg gcgccaaatc tggaagaggc
     3961 atctcggcgg atcactcctt caacctggta cttgttttct ttaaacccgt agcttgctga
     4021 tccgaaaaac gataacatat tggtggttac tatatttgaa taaccattaa tgtccacttt
     4081 ttgaaatccc tgtattactt tcaaatagtc cgacgtccca ttatccgccg taatccagga
     4141 catttgctgc acatctgaat ttaactcggt accggcaaga aaggt
//
LOCUS       DAOSDR010000286         4158 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrimonas sp. isolate AMR_MDS_1826
            NODE_7558_length_4158_cov_2.397270, whole genome shotgun sequence.
ACCESSION   DAOSDR010000286 DAOSDR010000000
VERSION     DAOSDR010000286.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863355
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrimonas sp. (wastewater metagenome)
  ORGANISM  Petrimonas sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Dysgonomonadaceae; Petrimonas.
REFERENCE   1  (bases 1 to 4158)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4158)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:10:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,720
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 35
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4158
                     /organism="Petrimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7558_length_4158_cov_2.397270"
                     /isolate="AMR_MDS_1826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2023866"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1000
                     /locus_tag="PLI30_10550"
     CDS             <1..1000
                     /locus_tag="PLI30_10550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009276496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HOI80031.1"
                     /translation="YLPGQGDLTEETYKNFSEFLDGCENNPFFLWIGFVDPHRPYNRS
                     VCPERNNPDDVIVPPFLIDGPDTRRDLADYYDEITRMDENMGKMISILEEKGKLDNTI
                     VVFLSDNGKPFPRCKGSLYDSGIQTPLIFMWKEKINPNTVHDNGLISCVDLAPTLLGL
                     VDIPLPQNIYGNSFHEIVFDHTKRGRDYIFAERNWHDTDEYIRCIRTEKYKLIYNAYF
                     EVPHGTASDLSSSASWYELRKQQNNGLLTKDQMQVFTAPRPMVELYDLQNDSLELNNV
                     ADVQEHIQEGRKLAKLLVQWQKETLDHPSWKRKRADKNDRITGFLLFDEIPELREE"
     gene            1135..1938
                     /locus_tag="PLI30_10555"
                     /pseudo
     CDS             1135..1938
                     /locus_tag="PLI30_10555"
                     /inference="COORDINATES: protein motif:HMM:NF027552.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4886 domain-containing protein"
     gene            1963..3030
                     /locus_tag="PLI30_10560"
     CDS             1963..3030
                     /locus_tag="PLI30_10560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008024331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HOI80032.1"
                     /translation="MSEYSWRTIAPYFYPPKEYEYSFHGYRSPLQFYDGTLVKDRTDW
                     RKRREEIRSRWMEMLGEWPPILDQQEFKIIDKEKREDFTQYRVRFYWTPNEQTEGYLL
                     IPDKKGQKPAVISVFYEPETAVGIGGKPYRDFAYQLVKKGFVTLSLGTSETTKNKTYS
                     IYYPNRESAIIQPLSALAYAAANALEALTKVEDVDAERIGIVGHSYGGKWAMFASCLY
                     DKFACAAWVDPGIVLDETKGSGVNYWEPWYLGYYQPPWKQTWSKTGENGRGVYPKLKK
                     EEYDLHELHALMAPRPFLVSGGSSDQVERWIPLNHTVAVNKLLGYTHRVAMTNRPEHS
                     PNAESNKVLYAFFEWFLTPNE"
     gene            3387..3665
                     /locus_tag="PLI30_10565"
                     /pseudo
     CDS             3387..3665
                     /locus_tag="PLI30_10565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018360703.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acetylgalactosaminidase"
BASE COUNT         1311 a          725 c          879 g         1243 t
ORIGIN      
        1 atatcttcca ggccaaggtg atttgacaga agaaacctat aaaaattttt ctgaattttt
       61 ggatggttgt gagaataatc ctttttttct ttggataggt tttgtagatc cacaccgtcc
      121 ttataataga agtgtttgtc ctgaaaggaa taatccggac gatgtgattg ttcctccttt
      181 cttgattgat ggtccggata cccgtcggga tctggcagat tactatgatg agataacgag
      241 aatggatgaa aatatgggca aaatgatatc catcttggag gagaaaggaa aacttgataa
      301 tacaattgtt gtttttctta gtgacaatgg aaaacccttt ccaaggtgta aaggttcact
      361 ctatgattct ggaattcaga ctccgcttat ctttatgtgg aaagagaaaa ttaatcctaa
      421 tacagtacat gataatggtt taatcagttg tgttgatcta gctcctacgc ttttaggcct
      481 tgttgatatt ccgttacctc agaatattta cgggaacagc tttcatgaga ttgtttttga
      541 tcatactaaa aggggaagag attacatctt cgctgaacga aactggcacg acacagatga
      601 atacatccgc tgtattcgca cagagaaata taaacttatt tataacgcct actttgaagt
      661 tccgcatgga acagcatctg atttgagttc aagcgcaagt tggtatgaat tgagaaagca
      721 gcaaaataat ggtctcttga caaaagatca aatgcaagta tttactgctc cccgtccgat
      781 ggtagaactg tatgatttgc agaatgattc tcttgaattg aacaatgttg ccgatgtaca
      841 agaacatata caggagggta ggaaattagc caagttgctc gtccaatggc aaaaggaaac
      901 tcttgatcat ccatcctgga aaagaaaacg ggccgataaa aatgatcgta taaccggatt
      961 tttattgttt gatgaaattc ctgaactgag agaagaataa tgcaattaaa tgatgtttgt
     1021 taaggtgaaa tctgctaggt tgtttgattg aagtgagttg atttttgata gcctgtgggc
     1081 ttttatcgta acacaggtga aagtataata aacagattat aatccataat gatgatgaac
     1141 aaattcaccc tgttttttct tgtgtttggt gtctcaattt tatctgttca tgcgaaacag
     1201 ataaaactcc ttgcgatagg aaacagcttc tcagacgatg caattgaata ttatctctcc
     1261 ggattagctg aagcaaatgg agataccatt atactcggaa acatgtacat tggcgggtgc
     1321 tctttagaaa agcattacaa caattctgta aataattccc ccgactactc atatcgaaaa
     1381 attgttaacg gggttaagac tacgacaccc ggttacacgc tgatcgatgc ttttggagat
     1441 gaagagtggg actacatctc ctttcaacaa gtaagttccc tttccggaca atatgagtcc
     1501 tatttcccat accttgaaga actgattagt tttgcagaaa aatactcctc caatccggat
     1561 atggagccca ttttacacgc tacctgggca tatgcacaaa cttctacaca tgcgggtttt
     1621 cagaattaca gtaacaatca gggttcgatg tacaatgcca ttattgatgc ctctggccgt
     1681 gcagcacaaa aaacgggaat caagattatt atcccggccg gtacggctat acaaaacggt
     1741 agaacaagta gtctgggaga cactttttgc agagatggct atcatctgga actgaactac
     1801 ggaaggtata cggcttcttg tgcatggtac gagaaactat ttaaaaaaac ggttgtagga
     1861 aacagttatg tgccctgtac ggttactccc tttcaggcaa agatagctca gctttccgcc
     1921 cattatgctg tagccaatcc cgataaagta acgcctgttg aggtgagtga atattcctgg
     1981 cgtactattg ctccctattt ctatccaccc aaagagtacg agtatagttt ccacggatat
     2041 cgctctcccc tgcagtttta cgacggtact cttgtaaagg acagaacaga ttggagaaag
     2101 aggcgtgaag agatccgttc ccgctggatg gagatgttgg gagaatggcc acctatattg
     2161 gatcaacagg aatttaaaat tatcgacaaa gagaaaagag aagattttac acaatacaga
     2221 gtccggtttt attggacacc caatgaacaa acggaaggat atttactgat tcctgataag
     2281 aaaggtcaaa agcctgccgt gatatctgtt ttctatgaac cggaaacagc agtgggaata
     2341 ggaggaaaac cttataggga ttttgcgtat caattggtaa aaaaaggatt tgttactctc
     2401 tccctaggta cttctgaaac aacgaaaaac aaaacatatt ccatctatta tccaaataga
     2461 gagtctgcaa tcatacaacc tttatcagca ttggcctacg ctgcagcaaa tgcgctggaa
     2521 gcactaacaa aagtagagga tgtagatgcg gaacggatag gaattgtcgg gcactcatac
     2581 ggagggaagt gggctatgtt tgcctcctgt ctgtacgata agtttgcttg tgcagcgtgg
     2641 gttgatcccg gcatcgtttt ggatgagacc aagggttccg gggtaaatta ttgggaaccg
     2701 tggtacttgg gttattatca accgccttgg aaacaaacat ggagcaagac aggtgaaaat
     2761 ggccgggggg tgtatccaaa gcttaaaaaa gaagaatacg accttcatga actacatgcc
     2821 cttatggctc cacgtccttt tcttgtgtct ggaggatcgt ccgaccaggt tgaacgttgg
     2881 attcctctga atcataccgt tgctgttaat aaattgttgg gctatacaca ccgggttgct
     2941 atgaccaacc gccctgaaca tagcccaaat gcggaatcaa ataaagtgtt gtatgctttt
     3001 tttgaatggt ttctcacacc aaacgaataa tcatttatta ctttttcagc tatttttccg
     3061 ttttttaagg aatagtctgc ggattaatct gatctatctt tttcattcct ttcttccttt
     3121 gtaatttctg acattgacta tttgggtgca attaaaaagg ggataaatgc ttaatatcgg
     3181 cttctcctct ttttctgctt tattgtccat aaggggagca atgtttttta actttcaaat
     3241 tttgtttaga aatgacagaa catcttgctt cccaggttga tgcaatgaaa ttaaaagaaa
     3301 ggaaaatata aagaatagat aataaaggaa ataaatatag taggctatag ctataaatat
     3361 actatagtaa tagaacgttt aatgacctga tgagacagta tgagcacccc ttcataaaag
     3421 agataggaga gaaagctaaa aaagtgggcg gacacggagg aatggactac atgatggatt
     3481 atcgtttgat ttattgctta agaaatggct tgcctcttga tatggacgta tatgatgccg
     3541 cagaatggtc atgtctcgta gaacttacaa aaacatccac taccaatgga gggcagccgg
     3601 tgaaaattcc cgatttcacc aggggagatt ggaatgagtt acaaggattg gaattctttc
     3661 agtaaaaata gtgtaaatcg gaaaaaaagt ggactttctg ttggtgttgt gggaattcaa
     3721 caaaaacgaa acaacagaca ttactacatg gttaccaaaa aatatcaggt tggaatttac
     3781 acctcttttg ggtgatggca aaaaagtaga caaacggtaa aagacaacgg cggactttct
     3841 gttaaattac ccaaagaaaa tagctttgtc ttatataagt ataacataat taattgatta
     3901 ttatataatc aatcgatgtg gttgtagact ttttttgaat aaatatcagt ttgtaaataa
     3961 aaacgttttt tgaagatttc ttttcgcaaa aacaggagat tttatctttt aaaatatatt
     4021 cctttatatt taataatttt aatgtattga atttaaatct gtcaagctat atgcatcaga
     4081 aacaggccct tttgtttcac agttagttaa tgattcatat gcacatgata agggtctgcc
     4141 gtttcatgat ttttgtgg
//
LOCUS       DAOSDR010000287         4155 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrimonas sp. isolate AMR_MDS_1826
            NODE_7569_length_4155_cov_4.322927, whole genome shotgun sequence.
ACCESSION   DAOSDR010000287 DAOSDR010000000
VERSION     DAOSDR010000287.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863355
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrimonas sp. (wastewater metagenome)
  ORGANISM  Petrimonas sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Dysgonomonadaceae; Petrimonas.
REFERENCE   1  (bases 1 to 4155)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4155)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:10:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,720
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 35
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4155
                     /organism="Petrimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7569_length_4155_cov_4.322927"
                     /isolate="AMR_MDS_1826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2023866"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1439
                     /locus_tag="PLI30_10570"
     CDS             <1..1439
                     /locus_tag="PLI30_10570"
                     /inference="COORDINATES: protein motif:HMM:NF017280.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="acetylxylan esterase"
                     /protein_id="HOI80033.1"
                     /translation="GIQTLLTGSSISDYFIWDGVRAVDFLETRKEVDMNRIGITGRSG
                     GGTQTALIAAYDERIHAAAPECYITTFKRLLQSIGPQDAEQNPYMSIKKGIDIPDLLH
                     MRSPKPTLIVTTTHDFFSQQGARETFKEAKKSYNALGIADNILFVEDMGGHQSTKKNR
                     EAVNSFFMKFLNLPGDPTDHEVTLFKDEELWATSTGQVASSLKGNTVFELNQRYYSAK
                     SLPQSSVKDKIKTTAGINFDRKLTAAVFTGKFSKGETVVEKYFIENDKNDYALPVYVI
                     KKEGMKAQNVVVWNHPSGKEKLLEEPTLMNILNAGNVVVSADLPGIGELYDPEFRGDG
                     FVQGVPFNYIFIANLTGKSIPGVQAEAIDLMMQFVASNDNFNDATLNVVTEGTLISPS
                     LLHAIFKNTFKKIAVLDPPPPGSYFVETKYYDPLFAFSIVPGSVGSYEWKDLLAFLPN
                     TSVKIFAAKNKQQPDKINQLEIVDFLRK"
     gene            1467..2693
                     /locus_tag="PLI30_10575"
     CDS             1467..2693
                     /locus_tag="PLI30_10575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HOI80034.1"
                     /translation="MKTNLIQRLFLLSLLTGLLAVNSCTSKEKKNSVNVTQTDSTMLC
                     VGYYWTEAEGKEFLEKQRREYTTAEDWKRRAEKIRNQILKGTGLEKFPEKCPLNPIFG
                     DKRTYEGYQVQNVAFESLPGVYVTGSLYTPANAAKNLPGILNTHGHWAELDDYGRYRP
                     DAQKRFAAMARMGAMVFAYDMVGYGQLAREHGWIHKHPEALKLQLWNSIRSIDFLLSI
                     GADPERIAITGASGGGTQAFLLTAVDDRIAVSVPVVQVSAHFFGGCVCESGMQIHKTD
                     NFQTNNVEIAACAAPRPMLVVSDGDDWTKNTPSVEYPHLQYIYGLFGKFQVVENAHLP
                     ADKHGYDYNKRVAVYPFLAKHLGLDVTKAVNPDGSLNEQNIVIEDIEALYPFDEKRPF
                     PAHGIKTNDAVIWNSN"
     gene            2810..4063
                     /locus_tag="PLI30_10580"
     CDS             2810..4063
                     /locus_tag="PLI30_10580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010266639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOI80035.1"
                     /translation="MTSRRQFLKTLGGVTLLTVVPRNVLGKGFVAPSDELTKGIIGVG
                     GMGRGHISYDKTRVVAMCDVDKNHLNQAAALVKDNISLYHDFRELILDKNVDIVHIAT
                     PPHWHGIMSVEAAKAGKDIFCEKPMTRTIGESKRVVEAIQQYGNIFRLNTWFRFKDPF
                     YGLGTPVKPLKKLVDSGLLGWPLKVTISKHTGFDWKFYWVGKTHLEPQPIPEELDYDM
                     WLGPAPYKPYNAHRVHGTFRGYWDYDGGGLGDMGQHYIDPVQYLLGKDNTSPVKVEVD
                     APQQHPDAVGTWRSITYTYDDGCQIILWGGDYGEPNTPYIAGPNGNVYRNFVCDIPDW
                     QKKLADFPEPTEQNTNFIESIRNRQKFALNELNGHRSATIVNMGAVALRLNRTIEYDP
                     VKEIFVNDEEANRLSDQPMRAPWSI"
BASE COUNT         1284 a          896 c          930 g         1045 t
ORIGIN      
        1 ccggaatcca aaccttattg acaggatctt caatttcgga ctatttcatt tgggacggcg
       61 tacgggctgt tgactttctt gagacccgaa aagaggtgga tatgaaccgg ataggcatta
      121 ctggtcgctc aggaggaggc acgcagactg cattgatagc tgcctacgat gaacgtatac
      181 acgcagccgc ccccgaatgc tacattacta cattcaagag gttgctgcaa tcaattgggc
      241 ctcaagacgc agaacagaat ccttatatgt cgatcaaaaa gggaattgac attcccgatt
      301 tgctgcatat gcgctcgccc aagcccacat tgatcgtaac gactacacac gattttttta
      361 gtcaacaagg agcccgggaa actttcaaag aggcaaagaa atcatacaac gcactgggta
      421 ttgctgacaa cattctgttc gtagaagata tgggagggca tcaatcaaca aaaaaaaaca
      481 gggaggcagt taattcattt ttcatgaaat tcttaaacct gccgggagat ccaacagatc
      541 atgaagtaac tctctttaaa gacgaggaac tttgggcgac gtctacagga caagttgcca
      601 gttcattaaa aggcaacact gtttttgaat tgaatcaacg gtattattca gccaagagct
      661 taccgcaaag ctctgtaaaa gataaaatta aaaccacggc aggcattaat tttgatagaa
      721 aattaacggc tgcagttttt acgggtaaat tctcaaaagg agagaccgtt gtagaaaagt
      781 actttattga aaacgataag aacgactatg ctcttcccgt atatgtaatt aaaaaggagg
      841 gtatgaaagc acagaatgta gtggtgtgga atcatccttc aggaaaagag aagcttctgg
      901 aagaacccac cctgatgaat atactgaacg caggcaatgt cgttgtttca gcagatttac
      961 cgggtattgg cgaattatat gacccggagt tcagaggcga cgggtttgta cagggagtgc
     1021 cgtttaacta tatcttcatt gctaatttaa caggaaaaag cattccgggg gttcaagccg
     1081 aagcgattga tttgatgatg caatttgttg cgagtaacga caattttaac gatgcaaccc
     1141 tgaatgttgt aacagaaggc acattgattt ctccatcttt gctccatgca atatttaaaa
     1201 acaccttcaa gaaaattgcg gtgctggatc cgccgcctcc gggcagttat tttgtagaaa
     1261 cgaaatatta cgatcctctt ttcgctttct ctattgttcc ggggagcgtt ggttcctatg
     1321 agtggaaaga cttgcttgcc tttttaccaa atacctcggt caaaattttc gcagcaaaaa
     1381 acaaacaaca acccgataaa atcaatcagc ttgaaatcgt cgatttctta cgaaaataat
     1441 aactttttca tcattattca ttgaaaatga aaacaaacct tattcaaaga ctttttttat
     1501 tatccttgct gactgggctt ctcgctgtaa attcctgtac atccaaagag aaaaaaaaca
     1561 gcgtaaacgt aacacaaacg gattctacca tgctttgcgt tggatactat tggaccgagg
     1621 cagaaggaaa agaatttctg gagaaacaac ggagagaata cactactgct gaggattgga
     1681 aaagacgcgc ggaaaagata agaaaccaga tcctgaaagg aacggggttg gaaaaatttc
     1741 ctgagaaatg tccccttaat cctattttcg gagataaacg cacttacgag ggttaccaag
     1801 tacaaaatgt tgcctttgaa agtctgcctg gagtatatgt taccggaagc ctttatacac
     1861 cggcaaacgc tgctaaaaat ttaccgggaa tcctcaatac gcacggacac tgggcagagc
     1921 ttgatgatta cggccggtat cgtcctgacg cacaaaaaag gtttgcagct atggccagga
     1981 tgggcgcaat ggtttttgcg tacgacatgg tcggatatgg acaactggca cgggaacatg
     2041 gatggataca caaacacccg gaggcattga aattacaact ctggaacagc atccgaagca
     2101 tcgatttttt actttccatc ggtgccgatc cggaaaggat tgccattacc ggagcttcgg
     2161 gcggcggcac gcaagcattc ttactcacgg ctgtcgatga cagaattgcg gtttctgtcc
     2221 ccgtagttca ggtctctgcc catttttttg gtggatgcgt ttgtgaaagc ggtatgcaga
     2281 ttcacaaaac agataatttc caaaccaata atgtagaaat tgcagcctgc gctgctccgc
     2341 gaccaatgtt ggtggtttct gacggtgatg actggacaaa aaacactcct tctgttgaat
     2401 accctcacct tcagtacatt tacggacttt ttggcaagtt tcaagtcgtt gaaaacgctc
     2461 atttacctgc cgacaaacac ggttacgatt acaacaaacg ggtcgcggtt tatccctttt
     2521 tagctaaaca cctggggtta gacgttacga aagcagttaa tccggatggg tctttaaatg
     2581 agcagaatat cgttattgaa gatatcgagg ctctgtaccc ttttgatgaa aaaagacctt
     2641 tcccggctca cggaataaaa acaaacgatg ccgttatctg gaactcgaat tagtcggaaa
     2701 aactcaaaag aaaataaaag tatttaacaa gtcacaacaa aagatgagca tattcaaaaa
     2761 ttgattatat ttacaaaatt taattttatt atacattaca acttaattta tgacctccag
     2821 aagacaattt ttaaaaaccc tcggtggggt aacgcttctc actgtggttc cccgaaatgt
     2881 attggggaaa gggtttgtcg cacccagcga tgaactgaca aaaggtatta tcggcgtagg
     2941 cggaatgggt cgcggacaca tttcttatga caagacgcgc gttgtagcca tgtgcgatgt
     3001 cgataagaac caccttaacc aggccgcagc cctggtaaaa gacaacatca gcctctatca
     3061 cgactttcgt gagcttatcc tcgacaaaaa tgtggatatt gtgcatattg cgactccgcc
     3121 ccactggcac gggattatgt cggtagaagc agccaaagca ggaaaagata ttttctgcga
     3181 aaagccgatg accagaacta tcggcgaaag caagcgtgtt gttgaagcca ttcagcaata
     3241 cggaaatata ttcaggttga atacatggtt ccggttcaaa gacccgtttt atggactggg
     3301 cacgccggta aagccactca aaaagcttgt cgacagcgga cttctcggct ggccgttaaa
     3361 agttactatc agcaaacata ccggttttga ctggaaattc tactgggtag gaaaaacaca
     3421 tcttgaaccg caacccattc ccgaagaact cgattacgac atgtggttgg gcccggctcc
     3481 ttacaaacca tacaatgcac atcgcgtgca cggaacattc cgcggatact gggattacga
     3541 cggcggtggt ctgggtgata tgggtcaaca ctatatcgat ccggttcagt atttgttagg
     3601 aaaagacaat acaagcccgg taaaagtaga agtggatgct cctcaacaac atcccgacgc
     3661 agtaggtacc tggcgttcca ttacatacac ctatgacgac ggctgccaga ttattctgtg
     3721 gggaggcgac tacggtgagc ctaatacgcc ctatattgcc gggccaaacg gaaatgtcta
     3781 cagaaacttc gtctgcgaca ttcccgactg gcagaaaaaa ctggccgatt tccccgaacc
     3841 gacagaacaa aataccaatt tcatcgaatc aatacgcaac cgacagaaat tcgctttgaa
     3901 cgaattgaac ggacaccgtt ccgctactat tgtgaacatg ggtgccgtag cactccggtt
     3961 aaacagaaca atcgaatacg atccggtgaa agagattttt gtgaacgacg aggaagccaa
     4021 tcgcttatcc gatcagccaa tgcgtgcgcc gtggagtatt tagattgaac aaggtcgaac
     4081 tagattgaat aagattgaaa cataggcagc ccgccatcta tctttaccaa tctttgtcaa
     4141 tcttataaaa caatt
//
LOCUS       DAOSDR010000288         4122 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrimonas sp. isolate AMR_MDS_1826
            NODE_7634_length_4122_cov_4.590607, whole genome shotgun sequence.
ACCESSION   DAOSDR010000288 DAOSDR010000000
VERSION     DAOSDR010000288.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863355
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrimonas sp. (wastewater metagenome)
  ORGANISM  Petrimonas sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Dysgonomonadaceae; Petrimonas.
REFERENCE   1  (bases 1 to 4122)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4122)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:10:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,720
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 35
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4122
                     /organism="Petrimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7634_length_4122_cov_4.590607"
                     /isolate="AMR_MDS_1826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2023866"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1387)
                     /locus_tag="PLI30_10585"
     CDS             complement(<1..1387)
                     /locus_tag="PLI30_10585"
                     /EC_number="2.7.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019537839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008741 - ribulokinase activity [Evidence
                     IEA];
                     GO_process: GO:0019569 - L-arabinose catabolic process to
                     xylulose 5-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulokinase"
                     /protein_id="HOI80036.1"
                     /translation="MEKLVIGIDYGTDSCRALVVDAVSGKEMAAHVAFYPRWKKALYC
                     DASKNQYRQHPQDYIDTLEQAVKGALGQLPADAARQVIGIGIDTTGSTPCLTDRQGTP
                     LALLPDYADNPNAMFILWKDHTSIQEAGEINQLAYRWEIDYTSRSGGIYSSEWFWAKA
                     LHALREDESIRKDAYSIVEHCDWMPALLTGNLKPEELKRSRCAAGHKCMWAEEWDGYP
                     SQEFLSELDPLLNGFAAHLSAYTYTSDASAGKLSEEWAGRLGLPGGIDVAVGIIDAHA
                     GAIGAEIKANTMVKILGTSTCDIVVTPKSDIEDRLIPGISGQVDGSVIPGLVGLEAGQ
                     SAFGDVYAWLRDLLSWSFAVLPAGDVENATGKILQKLTEEAQDLPLTDDGITSSDWFN
                     GRRTPFADQTLKGGITGLTLGTTPAQIFRSLVEATAFGSKAIMDHIEKEGVKVTEVIG
                     VGGISLKSPYVM"
     gene            complement(1387..2832)
                     /gene="fucP"
                     /locus_tag="PLI30_10590"
     CDS             complement(1387..2832)
                     /gene="fucP"
                     /locus_tag="PLI30_10590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019538170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015535 - fucose:proton symporter activity
                     [Evidence IEA];
                     GO_process: GO:0015756 - fucose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose:H+ symporter permease"
                     /protein_id="HOI80037.1"
                     /translation="MNSNVEKHRLVPAGIVFPFILLTSLFFIWAVPNNMTDTMLAAFK
                     RIMSLSDTQTAWIQVVCYLLGYGCFALPGAIFIKKHTYKYGVLLGLGLCIFGGVLFYP
                     AMLANNISSPLSFATYLLAIFVLFAGLSVLETSANSYVYALGDPHTATRRLNFSQSFN
                     PFGALTGVLASQIFVLSQLNTMSAAERAVLNEAELVNVQNAELNAVTIAYLILGLVML
                     ALFLLIFFTKMPQAQDEDKTLNLKATFNRLKRNKNYVWGVVAQFFYVGAQIAVWSYII
                     RYAMQQLQFDAVISGLGNNASQESIIAALRSVEPIAGGFYNLAESLGIDALLPRTAEQ
                     AGATYYVLSLVLFVVGRFVCTFLMKWTRPVNLLSALSVLAVVCSLVAIYGQGFIGVYA
                     LMGISGCMSLMFPTIYGIGMKGLGEDTKIAGSGMVMAIAGAALLTQMQGILSDQAGSI
                     EFAYWVPTIAFIIIAFYSFTVARSKKIKING"
     gene            complement(2932..>4122)
                     /locus_tag="PLI30_10595"
     CDS             complement(2932..>4122)
                     /locus_tag="PLI30_10595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019538171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI80038.1"
                     /translation="SCTGNAKKSSDNMKKEYEKGTFGYDLNYLSKKDSLIVLKSDDGK
                     AQVIVSAKYQAKVFTSTANGEEGNSLGFVNYKFFDAGTVDEHMNGFGGENRLWLGPEG
                     GQYSIYFKPGKEQVFDNWHTPKAIDIEEWKVDRATQEETVLTKYMELENYQGSILNIT
                     VERKISLVTEAEMASKLGVTLPDGVSAVGYATENKITNKNDFEWTSKTGTVCIWMLDM
                     FNPSDSAVTVVPYNEGDEGQLGTLATTDYFGEIPADRLKAENGTLYLKTDGKFRSKLG
                     MNAKRTKAIAGNYDPISKRLTVITFDVDPSAVYLNQEWNPARNPLTGDALNAYNDGPL
                     EDGSIMGPFLELESCSPAAFLKPGESLSHVHNVYHFVGDEAVLSPVCEKLLGVSIHQV
                     TTIF"
BASE COUNT         1070 a         1071 c          867 g         1114 t
ORIGIN      
        1 gcatcacata aggcgatttt aatgaaatcc ctcccacgcc aatcacttcg gtcactttca
       61 ccccttcttt ttcaatatga tccataatgg ctttcgaacc aaaggcggta gcttccacca
      121 gactcctgaa aatctgagct ggtgttgttc cgagtgtcaa tccggtaatt ccccccttta
      181 aggtctggtc ggcaaagggt gtgcggcggc cgttgaacca atcgctggag gtgatcccgt
      241 catcggttaa cggaagatcc tgcgcctctt cggtcagttt ctgaagaatt ttcccggtgg
      301 cattctccac atcccctgcc ggcaaaactg caaaactcca cgaaagcaaa tcccgtaacc
      361 aggcatatac atcgccgaat gccgattgcc ctgcttccag tccgacaagg cccggaatga
      421 cagagccgtc tacctgccca ctgatgccgg ggatcaacct gtcctctatg tcggacttgg
      481 gagtaaccac aatatcgcac gtggaggttc ccagaatctt aaccatcgtg tttgccttta
      541 tttctgctcc gattgctccg gcgtgagcat cgatgatgcc tacagccaca tcaatccctc
      601 cgggcaatcc gagtcgccct gcccactctt cagacagttt gccggcactt gcgtcactcg
      661 tgtacgtata agcgctcaaa tgggctgcaa acccgtttag cagcggatcc agttccgaga
      721 ggaattcctg ggaaggataa ccgtcccatt cttcggccca catgcatttg tgtccggccg
      781 cacagcggct acgtttcagt tcttcgggtt tcaaattccc ggtaaggagt gccggcatcc
      841 aatcacaatg ttcaacgatg gaataggcat ctttacgaat cgattcatct tcgcgcaagg
      901 cgtgcaatgc cttagcccag aaccattccg atgaataaat tcctcctgaa cgggaagtat
      961 aatcaatctc ccaccggtaa gcgagttggt tgatttcccc agcctcctgg atggaagtat
     1021 gatctttcca caaaataaac atggcgttgg gattatcggc ataatcaggc aacagggcca
     1081 ggggagttcc ctgtctgtcg gtcagacagg gtgtgctacc agtggtatcg atgccgatac
     1141 cgataacttg ccgggccgca tccgcaggaa gttgtcccaa tgctcctttt acagcctgtt
     1201 ccagcgtatc gatataatct tgcggatgct ggcggtattg attcttggaa gcatcgcagt
     1261 acaatgcttt tttccaccgg ggataaaatg caacatgtgc agccatttct tttcccgaaa
     1321 cagcatcaac gactaacgcc cggcaggagt ccgttccgta atcgattcct ataactaatt
     1381 tttccattat ccgttaattt tgattttttt acttcgtgct accgtaaagc tgtaaaacgc
     1441 aatgatgatg aaagcgatgg tgggcaccca atacgcaaat tcgatacttc ctgcctggtc
     1501 ggaaagaatg ccctgcattt gagtcaatag tgcagcaccc gcaatagcca tcaccatccc
     1561 cgatcctgca atcttggtat cttcccccag tcctttcatc ccaattccat aaatggtggg
     1621 aaacatcagc gacatacagc ccgatattcc cattaaggca taaacaccaa taaatccctg
     1681 accgtaaata gcaaccaatg aacaaaccac cgcaagtact gaaagggctg aaagtaaatt
     1741 taccggcctg gtccatttca tcaggaacgt gcacacaaaa cgtcctacta caaacaaaac
     1801 aagagaaagt acgtaatagg tggccccagc ctgttcggca gttctcggca gcaaagcgtc
     1861 aatccccagc gattccgcca agttataaaa tccgccggca atgggttcaa cgcttcgaag
     1921 agctgcaata attgattcct gagatgcgtt atttcccaag cccgagatta cagcatcaaa
     1981 ctgcaattgc tgcatggcgt accggatgat atacgaccaa actgcaattt gcgcaccgac
     2041 gtaaaaaaac tgtgctacaa caccccaaac gtagttttta tttcttttca agcgattgaa
     2101 ggtcgctttc aggttcaggg ttttgtcctc atcctgtgcc tgaggcattt tagtaaaaaa
     2161 gattaacagg aaaagggcaa gcattaccaa acccagtatg agataggcta tggtgaccgc
     2221 attcaactct gcattctgaa cgtttaccaa ctcggcttcg ttcaacactg ctctttccgc
     2281 tgcactcatc gtatttaatt gcgagagtac aaagatctgg cttgccagta ctccggttaa
     2341 tgcaccgaaa gggttgaatg actgcgagaa attcagtcgt cgcgtggccg tatgcggatc
     2401 gcctaatgca tacacgtagg aatttgccga tgtttccaat accgacagtc ccgcaaaaag
     2461 gacaaaaata gccagcaaat aggtagcgaa gctaagcggt gagctgatgt tgttagccaa
     2521 cattgccgga taaaacaata ctcctccgaa gatacataat cccaatccca acaatacacc
     2581 atacttgtac gtgtgttttt taatgaaaat ggcacccgga agggcgaagc acccataacc
     2641 gagcaaataa caaaccacct ggatccaagc tgtttgcgta tccgacaagc tcatgatccg
     2701 cttgaaagcg gccagcatgg tatcggtcat gttgttggga accgcccaaa tgaaaaatag
     2761 ggaagtaagc aggataaacg gaaacacaat tcccgcaggc actaaacgat gtttttctac
     2821 gttgctattc atacgaataa aattattgat tttaaatgtg ttgcatggaa cttgctttta
     2881 atcatttgct cgggtgcaac tgatttacat gctcgttcca tactgttttt ttcagaagat
     2941 ggttgtaact tgatggatac ttactccgag caatttttcg caaacaggcg acaagactgc
     3001 ttcgtcacct acaaaatggt aaacattgtg tacatgcgac aatgattctc ccggttttaa
     3061 aaatgctgcc ggagaacagc tttccagttc caggaaaggc cccatgatgc tgccatcttc
     3121 cagcgggcca tcgttatacg cattcagggc atctcctgta agtggatttc ttgccggatt
     3181 ccattcctgg ttcaggtaca ctgcagaagg atcaacatcg aacgtaatta ccgtaagtct
     3241 cttggaaatc gggtcgtaat tgcccgcaat agcttttgtt cgtttagcat tcatccccag
     3301 tttgcttctg aattttccgt cagttttcag gtagagcgtt ccgttttcgg ctttcagcct
     3361 gtcagctgga atttcaccga aatagtccgt cgttgccagt gtccccagct ggccctcatc
     3421 tccctcgtta tatggaacaa cggttactgc ggaatccgaa ggattgaaca tatccaacat
     3481 ccatatacaa acggttcctg ttttgctggt ccattcaaaa tcattcttgt tggtgatttt
     3541 attctcggta gcataaccaa cagcgcttac tccatcgggc agggttacac ccagtttgct
     3601 tgccatttct gcttcggtaa caagcgaaat tttcctctct acggttatat ttaagatgct
     3661 tccctgataa ttttcaagtt ccatatattt ggttaacaca gtttcttcct gagtagcacg
     3721 gtccactttc cactcctcta tgtctatggc tttaggggta tgccaattat cgaaaacctg
     3781 ttcttttccc ggtttgaaat agatagaata ctgtcctccc tcggggccca gccacaaccg
     3841 attctcccca ccaaatccgt tcatatgctc atccactgtt ccggcatcaa agaacttgta
     3901 attcacaaaa cccagactat ttccttcttc accgtttgcc gttgaggtaa agacttttgc
     3961 ctgatatttg gcagacacga tcacctgtgc ttttccgtcg tcggacttca gcacaatgag
     4021 gctgtctttt ttcgatagat aattcagatc gtatccaaat gtcccttttt catactcctt
     4081 tttcatatta tccgatgatt ttttagcgtt acccgtgcag ga
//
LOCUS       DAOSDR010000289         4096 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrimonas sp. isolate AMR_MDS_1826
            NODE_7700_length_4096_cov_3.086365, whole genome shotgun sequence.
ACCESSION   DAOSDR010000289 DAOSDR010000000
VERSION     DAOSDR010000289.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863355
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrimonas sp. (wastewater metagenome)
  ORGANISM  Petrimonas sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Dysgonomonadaceae; Petrimonas.
REFERENCE   1  (bases 1 to 4096)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4096)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:10:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,720
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 35
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4096
                     /organism="Petrimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7700_length_4096_cov_3.086365"
                     /isolate="AMR_MDS_1826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2023866"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..657)
                     /locus_tag="PLI30_10600"
     CDS             complement(<1..657)
                     /locus_tag="PLI30_10600"
                     /inference="COORDINATES: protein motif:HMM:NF025680.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HOI80039.1"
                     /translation="MKKYKNIQYCLIAFLLSFILSSCENALDQKPVDSFNEESLFQDI
                     NLAEAFLYQCYDVMGGDREEVLGMREDLLSSSTDELLNIHRAGQVTFTKGNLTPDYLG
                     HFGNWRYGWILWDFNYSNIKNVNTLLGGIDEVPTQSKADEDKIEQIKAEAYFIRAFNY
                     TNLLRSYGGVVLIDKKFNLDDDFLSQERASLEETLNFILVDLDKAIAGLPDKNEIAQG
                     R"
     gene            complement(688..4044)
                     /locus_tag="PLI30_10605"
     CDS             complement(688..4044)
                     /locus_tag="PLI30_10605"
                     /inference="COORDINATES: protein motif:HMM:TIGR04056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOI80040.1"
                     /translation="MKISFLMLFLCFFTITAGNVFSQQKELSLELNNVTLKKAISEIE
                     KTSDYVFLITDEARRELNRKISVRAKKESIQTILKSIFNDTNLDYTTIERQISVYKNH
                     KPKTEESEKVAKIEPEQQKRTITGIVVDATGVSIIGANIIEAGTTNGTVTDVDGKFSL
                     SVDNNATIRISYIGYLEQNIGTAGQNSFTITLLEDVQTLQEVVAIGYGTQRKENLVGA
                     VSAVSGEEIQAVHSGDVTNAVSGRLPGAIVMQPSGEPGRNEAKILVRGRTTLGDNTGP
                     LVVIDGIPDRSLYEIDPNDIESISLLKDASAAIYGSQAANGVILVTTKSGSEGNWMSY
                     RFYQGFKTPTVLPKVANAGEYAQYISDYQDYEGLARLYSDKDVELYKNGKDPWEHPQT
                     DWMSDLVRKWTTESNHHLSLSGSHNKMRYYASLGFRNDQAMYAQESTNYKQYNLRLKL
                     DIPVTDWLETSVYYGGFMTTRKYPTTSTYQLVGWATMALPTQPSFWPTGEPGPDFEGG
                     VNPVVNTSFDAGYDQQDNYKNEVTFRGTFKPKKIKGFTLNGFYTFDVNNQNRKLFRKP
                     WTLYYPDWDSAVRNSEGYITSMELTPRKRGLENPELTEANFRYLKKMYNINATYEKDL
                     GDHAFSIFGAFEELKENIYGFDAYRKYFISDLVQTLDAGGEKDKTNSGQMSIYARQSW
                     IGRLNYNFREKYLLEFILRRDGSLKFPPNSRWGNFPAVLFAWRASEEDFWKNNLSVID
                     YFKLRTSYGQMGMDPGDPFQYINKYSLGTGMTFGSNKDVVTKIYQSVIANPKITWEKQ
                     TTFNFGFDSQFLNQMFHLNTEFFYNKRSDILAARDASVPAYTGLALPLENIAIVDNRG
                     FEIDAGFHKKITSDLSLDLTGNVSWNKNKIVFMDEPERAVPWQRLTGHPYGAQLVYKA
                     IGIFKTQAEIDAYPHWSGAKPGDVIFEDVSKDGKINADDRILLDKTDAPQVFYGMILD
                     LTYKNWALSLQGQGQGTYYKSPIEGNRGIGQNVYKWMATDYWTPENSNSNKARPFHRA
                     DQYWSYISNANTYWYDNMAYFRLKNVVVSYNLPKELTSKAGLANVNIFASGYNLFLIY
                     SKQRNYDPEVGNPQYYPAMRTVSLGVKVNF"
BASE COUNT         1125 a          840 c          731 g         1400 t
ORIGIN      
        1 tctgccctga gctatctcgt ttttatcagg taatccggca atcgctttgt ctaagtcgac
       61 taaaataaaa ttcaacgttt cttccaatga agctctttcc tgggataaaa aatcatcgtc
      121 taaattaaac tttttatcga taagcactac tcctccgtat gaacgaagga ggtttgtata
      181 attaaaagct cttataaaat aagcttccgc tttaatctgt tcaattttat cctcatcggc
      241 ttttgattgt gtaggaactt cgtcgatacc tcccaaaaga gtgttaacgt ttttaatatt
      301 agagtaatta aagtcccaca gtatccatcc atatcgccaa ttaccaaagt ggcctaaata
      361 atcgggagta aggttccctt ttgtaaaagt gacttgtcca gctctatgaa tattcagaag
      421 ttcatccgtt gaagatgaca ataaatcttc acgcatccct aaaacttctt ccctgtctcc
      481 gcccatcaca tcgtaacatt ggtatagaaa tgcttccgca agatttatat cctgaaagag
      541 cgactcttca ttgaatgaat ctacaggctt ttgatccaat gcgttctcac aagaggaaag
      601 aatgaaggag agtagaaatg caattaaaca gtattgaatg tttttatatt ttttcatatt
      661 atttgtgtga ttacagggtt atttatatta aaaattaacc tttactccca gtgatactgt
      721 tctcattgcc ggataatatt ggggattccc aacttccgga tcgtaatttc tttgcttgga
      781 gtaaatcaaa aataaattgt aacccgaggc gaaaatgttc acatttgcca atccggcttt
      841 acttgttaat tcttttggta aattataact tacaactaca tttttcaagc ggaaataagc
      901 catattgtca taccagtatg tatttgcatt gctgatataa gaccagtatt ggtcggctct
      961 atggaacgga cgagccttat tggaattact gttttccgga gtccaataat ctgttgccat
     1021 ccatttgtaa acgttttgtc cgattcctct gttgccttca attggacttt tataatatgt
     1081 accttgtcct tgtccttgta aagaaagcgc ccaattttta taagttaggt ccaagatcat
     1141 tccataaaaa acttgtggag catcggtttt gtccaaaaga atcctgtcat ctgcatttat
     1201 ttttccgtct ttacttacgt cctcaaaaat aacatctccc ggttttgccc cactccaatg
     1261 tggataagca tctatttcgg cttgtgtttt aaatatgcca atggctttgt aaaccagttg
     1321 tgctccgtat ggatgtcctg ttaatcgttg ccaggggaca gctctttcag gttcatccat
     1381 aaaaacaatt ttattcttgt tccagcttac gtttccagtt aagtccaaac ttaaatcaga
     1441 cgtaatcttt ttatggaatc cggcatcaat ttcaaatcct ctgttatcaa caattgcaat
     1501 attctccaac ggtaaggcta gacctgtata tgcaggaaca gatgcgtcgc gcgcagctaa
     1561 aatatcagaa cgtttgttat agaaaaactc ggtattgagg tggaacattt gattcaagaa
     1621 ttgagaatcg aagccgaaat tgaatgtcgt ttgtttttcc catgttattt tcggattggc
     1681 aataacagat tgatatatct ttgttactac atctttatta ctaccgaatg tcattccagt
     1741 tcccagactg tatttattga tatattgaaa agggtctccc gggtccatac ccatttgtcc
     1801 gtatgatgtt cttagtttaa aatagtcaat tacagaaagg ttgtttttcc aaaaatcttc
     1861 ttcagaagca cgccaagcaa acaaaactgc gggaaaattt ccccatctgc tgtttggtgg
     1921 aaatttcaag gagccgtctc ggcgtaagat aaactcgaga agatattttt ctctgaaatt
     1981 ataatttagt cttccaatcc acgattgtct tgcataaata ctcatctgcc cggagtttgt
     2041 tttgtctttt tctccccccg catcaagagt ttgaacaaga tcggaaataa agtattttct
     2101 gtacgcatca aatccgtata tattttcttt taattcttcg aaagcgccaa aaatagaaaa
     2161 agcatgatcg cctaggtctt tctcataggt tgcgtttatg ttgtacattt ttttcaaata
     2221 cctaaaattt gcttcggtaa gttccggatt ctccaatcct ctttttctcg gtgttaactc
     2281 catactggta atatacccct cagaattgcg caccgcggaa tcccaatctg ggtaatataa
     2341 agtccagggt ttcctgaaga gttttctatt ttgattgttc acatcaaaag tgtaaaatcc
     2401 gttaagagta aaacctttta tttttttcgg cttaaaggtt cctctgaatg ttacctcgtt
     2461 tttataatta tcttgctgat catagcccgc atcgaaactt gtatttacca ctgggttgac
     2521 tcctccttca aagtcagggc ccggttcccc ggtcggccaa aaagaaggtt gtgtgggtaa
     2581 tgccattgtt gcccaaccca ctaattgata agttgaggta gtgggatatt ttcttgtggt
     2641 catgaaccct ccataataaa ccgacgtttc cagccaatca gttacaggaa tatccaactt
     2701 taatcttaag ttatattgct tgtaatttgt agattcttgg gcgtacattg cttggtcgtt
     2761 tctgaagccg agagatgcgt aatatctcat tttattgtgt gaaccgctta aggatagatg
     2821 atggttgctt tctgtcgtcc attttctaac aaggtcactc atccaatcag tttgtgggtg
     2881 ttcccaggga tcctttccat ttttatataa ttcaacatct ttatcagaat aaagtcttgc
     2941 aagaccttca taatcttgat aatcgctgat atattgagca tattctccag cattggcaac
     3001 tttaggcaga accgtaggtg ttttaaatcc ctgataaaac ctgtaactca tccaattacc
     3061 ttctgagccg cttttggtcg ttactagaat tacaccatta gctgcctgag atccgtaaat
     3121 ggcggcagag gcatctttca atagagaaat actttcaata tcatttggat ctatttcgta
     3181 taagcttctg tctggaattc catcgattac aacaagtggt cctgtattat cgccgagagt
     3241 tgttctaccg cgtaccaaaa tttttgcttc attgcgacct ggttctcccg aaggttgcat
     3301 aacaattgct cccggtaaac gtccagagac agcgtttgtt acatcaccag aatgaactgc
     3361 ttgaatctct tcgcccgaaa cagcagatac agctccaacg agattttctt tacgttgagt
     3421 cccgtatccg atagctacaa cttcctgcaa tgtctgcaca tcttctaaca aggtgatagt
     3481 gaatgaattt tgtccagccg taccaatgtt ttgctctaaa tatccaatat aagaaatacg
     3541 aatagtggca ttgttatcca cgcttaatga aaatttcccg tccacatcgg tgaccgttcc
     3601 gttggttgtt ccggcttcaa taatgttagc cccgataatg gatacacctg ttgcatccac
     3661 gacgatcccg gtaattgttc ttttttgctg ttcaggctca attttggcaa ctttttcact
     3721 ctcctccgtt ttgggtttat gatttttata taccgaaatc tgtcgttcta tagtcgtata
     3781 atccaggtta gtatcgttaa agatactttt caggatggtt tgaatactct ctttctttgc
     3841 ccgcaccgat atttttcggt tcaattctct ccgtgcttcg tcggtaataa gaaaaacata
     3901 atcgcttgtc ttttcgattt ccgaaatagc tttttttagg gtgacgttat tcagctccaa
     3961 tgagagctct ttttgctggg agaaaacgtt acctgcggta atggtaaaaa agcagagaaa
     4021 aagcattaaa aatgaaatct tcatgatttt tagggttttg tcgcactttt taaatgctct
     4081 ctggttaaat cttatt
//
LOCUS       DAOSDR010000290         4088 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrimonas sp. isolate AMR_MDS_1826
            NODE_7723_length_4088_cov_4.247211, whole genome shotgun sequence.
ACCESSION   DAOSDR010000290 DAOSDR010000000
VERSION     DAOSDR010000290.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863355
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrimonas sp. (wastewater metagenome)
  ORGANISM  Petrimonas sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Dysgonomonadaceae; Petrimonas.
REFERENCE   1  (bases 1 to 4088)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4088)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:10:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,720
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 35
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4088
                     /organism="Petrimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7723_length_4088_cov_4.247211"
                     /isolate="AMR_MDS_1826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2023866"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..323
                     /locus_tag="PLI30_10610"
     CDS             <1..323
                     /locus_tag="PLI30_10610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009121808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1343 domain-containing protein"
                     /protein_id="HOI80041.1"
                     /translation="SFTPRSVPGAKNPPLLNRKCYGVDLRNVSYEQIWEYGFDLSYVI
                     DAYKNLKMGDKFFTNFFEKLVGVDYIRQDIIAGKSAREIKEKWFCDVLRFKQQRRPYL
                     LYDE"
     gene            431..1096
                     /locus_tag="PLI30_10615"
     CDS             431..1096
                     /locus_tag="PLI30_10615"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009137784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic pyrophosphatase"
                     /protein_id="HOI80042.1"
                     /translation="MVDKLSDPIGRLMGLRYKSHPWHGISIGDHAPEEVTAFIEVVPT
                     DTVKYEIDKISGYLRVDRPQKFSNVVPALYGFIPQTYCGKRVGDFCNKKTGRTDILGD
                     GDPMDICVLTEKDLSHGDLLVNAIPIGGFRMIDGNQADDKIIAILKNDTVYGHYDNID
                     DVPKIVIQRLEHYFLTYKDMPGEDRNTEITHVYGHDEALEVIRYAVQDYNERFENIGL
                     ILS"
     gene            1307..2371
                     /locus_tag="PLI30_10620"
     CDS             1307..2371
                     /locus_tag="PLI30_10620"
                     /inference="COORDINATES: protein motif:HMM:NF018178.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1080 domain-containing protein"
                     /protein_id="HOI80043.1"
                     /translation="MKSLTTFNLLLVIVLGCFWGSCSERKQDSGIAVPQNDVSSYLGQ
                     WTFDFGQRSVGWLQVRQEEGYLDADLMWGGGGVFYGLPYVYVAGGNLYLGRNSRNTVL
                     TRDAEGQPLLSRSYPAWIELTREGDSISGYYLSPKVDGTGLDSIYIRGAKLPEMQLAP
                     DLSKLSFGEPVRLIRNNGDLTGWRLIEEDLKNGWTMNDGVLTNNPVQTEGEEHIRYGN
                     LRTEDVYEDFNLKLEVNAPRGSNSGVYLRGMYEVQVADSYDRPLDWGGSMGAIFTRIT
                     PTVKAEKPAGEWQDLDITLYKRHITVKLNGVTIIENQPVYGATGGAIQSDITAPGPIY
                     LQGDHTAISYRNIVLTPIIN"
     gene            2394..3752
                     /locus_tag="PLI30_10625"
     CDS             2394..3752
                     /locus_tag="PLI30_10625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006843038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOI80044.1"
                     /translation="MKSENSKRISRREFIGTSALAIGGLTLVPAHAVTGLGHVAPSDK
                     LNVAGIGIGGMGSGDLEDVAKTENIVALCDVDWSSQVENVFKLYPKAQRYKDYRVMLD
                     REKDIDAVIVATPDHTHAVISMEAIRRGKHVYTEKPLTHTVYEARMLTEAARKHNVAT
                     QMGNQGQAGDGPRRLQEMIHDGAIGAIEEVHVWTDRPNRGLSDTYWPQGVKRPEDMPP
                     VPETLDWDLFTGPAPLRPYHPSYHPFRWRGWLDYGTGALGDIGCHSFDSIFRILKLKY
                     PISIQGTSTLVNGDSFPLGSMVTYDFPARGDMPPLRLSWYDGGLRPPRLAEIVPGVEM
                     GAGGVLYVGTKGVILGDRLLPQSLDDTYKRPEPYLKSSPGHRQEWILACKGGDPAGSN
                     FDWAGPLTETVLLGNIALRPELREKMSYQSLNFDPETLSFPNMPEADQFLHYEYRSGW
                     TL"
     gene            complement(3768..>4088)
                     /locus_tag="PLI30_10630"
     CDS             complement(3768..>4088)
                     /locus_tag="PLI30_10630"
                     /inference="COORDINATES: protein motif:HMM:NF013584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase family protein"
                     /protein_id="HOI80045.1"
                     /translation="MLKGARDNGIDKIMDTHRLDAIIAPTGSPAWKTDWVNGDNFGIS
                     SSSAAAIAGYPAISLPMGFVSGLPVNITFYGKAWSEPTLIEMAFAFEQGTKHRRKPVL
                     NTEN"
BASE COUNT         1082 a          884 c         1075 g         1047 t
ORIGIN      
        1 tttcctttac tccccgcagc gttccgggag ctaagaaccc gccgttgctc aacagaaaat
       61 gttacggtgt ggacctccgg aatgtttcgt acgaacagat ctgggaatac ggtttcgatc
      121 tctcctacgt tatcgatgcc tataaaaacc tgaaaatggg cgataaattt ttcaccaact
      181 tttttgaaaa attagtggga gtggattata tccgtcagga tattattgcc ggaaaatccg
      241 ctcgggaaat aaaagagaaa tggttttgtg acgtattgag gtttaagcag caacggcgcc
      301 cttatctgtt atacgatgaa tgatgggttt tttgaggatt taggtagaaa ttggctttag
      361 ctctgaaaac cctgccccga gatctcaaat tcgaaatagt ttttgtactt ttgtaaataa
      421 aagataattt atggttgata aactttcaga cccgattggc aggttgatgg gattacgtta
      481 caaatctcat ccgtggcacg gcatttctat cggtgatcat gctcccgaag aggtgacggc
      541 atttattgag gtggttccta ccgatacggt aaaatacgaa atagataaaa taagcggata
      601 cctacgggtg gatcgtccac aaaaattttc aaatgtagtt cccgctctgt acggatttat
      661 tccgcaaacc tattgtggaa aacgagttgg cgacttctgc aataaaaaaa ccggaaggac
      721 agacatcttg ggtgatggag accctatgga tatttgcgtg ttaacggaaa aagacctctc
      781 gcacggtgac ttgttggtaa atgctatccc tatcggaggt tttcggatga tcgacggtaa
      841 tcaggcagac gataagataa tcgccatcct gaagaacgat accgtttatg gacattacga
      901 caatatcgat gatgttccta aaattgttat tcaacgcctg gaacactatt tccttactta
      961 caaggatatg cctggcgaag accgcaacac cgagatcacc catgtttatg gacacgatga
     1021 ggcccttgaa gttatccgct acgctgttca ggactataac gagcggttcg aaaatatagg
     1081 actgattttg tcttgatcta ccgcattttg cgctttctat taactcccgt ctttctgtgg
     1141 gagtactgtt cttccgatta ccgggatgct taggaatacc atgaatcatg ctaaacgggc
     1201 tctttgccgg tattcagcct gacagatagc ccgggaagag gtgtctgaac gaccttgtat
     1261 tgctatacca agtgagaaat gaacgtaact ttttaaaact tattctatga aatcattaac
     1321 aacttttaat ttattgttag ttattgtgtt gggatgcttc tggggctcgt gcagtgaaag
     1381 aaaacaggac tccggaattg ctgttcctca aaacgatgtt tcttcctacc tgggacaatg
     1441 gactttcgat tttggtcagc gatccgtggg gtggctgcag gtgaggcagg aagaaggtta
     1501 tttggatgcg gacctgatgt ggggtggcgg aggtgttttt tatgggttgc cttatgttta
     1561 tgttgcagga gggaacctgt acttgggaag aaattcccgg aacacagttc tgaccagaga
     1621 tgctgaaggc caaccgctgt tatcgagaag ctatcctgcc tggattgagc tgacaaggga
     1681 aggggacagt atttcgggct actatttaag cccgaaggtt gatggaaccg gacttgattc
     1741 gatttacata agaggagcta aattacccga gatgcagctt gcaccggacc tgagcaagtt
     1801 gagtttcgga gaacctgtac ggttaatcag gaacaacggc gacctaaccg gatggaggct
     1861 gatagaagaa gacctgaaga acggctggac aatgaatgac ggtgtgctta cgaataatcc
     1921 ggttcaaaca gaaggagagg aacatatccg ttacggaaat ctccgtacgg aggatgtgta
     1981 tgaggatttt aaccttaagc ttgaggttaa tgcccctcgg ggaagcaaca gcggggtata
     2041 cctccgcggg atgtacgagg tgcaggtggc agactcctac gataggcctc tcgactgggg
     2101 cggcagtatg ggtgctatat ttaccagaat cactcctacg gttaaggctg aaaaaccggc
     2161 cggagaatgg caggatctag acattacgct atacaagcgt cacattaccg ttaaattaaa
     2221 cggagtaacc attatcgaaa atcaacccgt gtatggggca accggcgggg ctatccaatc
     2281 ggatattacc gctccgggcc cgatctatct tcagggagat catacagcta tttcttacag
     2341 gaacatagtg ttgactccca taatcaattg atcgttaata aatatctttt gtcatgaaat
     2401 cagaaaactc aaaaagaatc agtcggagag agtttattgg aacctctgcc ttggccattg
     2461 gcggactgac ccttgttccg gctcatgctg tcaccggact ggggcatgtg gccccgagcg
     2521 ataagctgaa cgttgcagga attggaattg gtggaatggg aagcggcgac ctggaggatg
     2581 tagccaaaac agaaaatatt gtggcactct gcgacgttga ttggagtagt caggtggaaa
     2641 atgtatttaa gctctacccg aaagcccaaa gatacaagga ctatcgtgta atgctggacc
     2701 gggaaaagga tatcgacgca gttatcgttg ccacacccga ccacacacac gctgttatca
     2761 gcatggaagc catcaggagg ggtaagcacg tttacacgga gaagcccttg acacatacgg
     2821 tgtatgaggc aagaatgctg acggaggcag ctcgcaagca taacgtggct actcagatgg
     2881 gaaatcaggg acaagccggt gacggcccgc gaagattgca ggagatgatt cacgatggag
     2941 ccatcggggc tattgaggag gtccatgtgt ggaccgatcg gcccaaccgg ggattatccg
     3001 atacatactg gccgcaagga gtaaaacgcc cggaagatat gcctccggta cctgaaaccc
     3061 ttgactggga tttgtttaca ggaccggcgc cgttacgtcc gtatcatcct tcttatcatc
     3121 ctttcaggtg gcgcggatgg ctagattacg gcactggagc gcttggggat atcggctgtc
     3181 actccttcga ttcgatattc aggatactga agctgaaata ccccataagc attcaaggca
     3241 cttccacact ggttaacgga gactctttcc cgctaggatc catggttacc tatgattttc
     3301 ccgctcgggg agatatgcct ccgctacgcc tgagctggta cgacggagga ttacgccctc
     3361 cccgcctggc cgaaatagtg cccggtgttg aaatgggagc gggaggggtg ctctatgtag
     3421 gcactaaagg agtgatactg ggtgaccgcc tcttgcctca gtcattggat gatacctaca
     3481 aaagaccgga gccgtatctt aaatcttcgc ccggtcatcg tcaggagtgg atccttgcgt
     3541 gtaaaggggg tgatcctgcc ggatcgaatt ttgactgggc agggccgctg accgaaaccg
     3601 tgttgctggg aaatatcgca ttaagacctg aattgagaga gaagatgagc tatcagagcc
     3661 tgaatttcga tccagaaaca ctttcgttcc ccaacatgcc ggaggcagat cagttcctgc
     3721 attatgaata caggagcggg tggacactct gaacgttgtt atggccttca attttctgta
     3781 tttaacaccg gtttgcgccg gtgttttgtt ccttgttcga aggcaaatgc catttcaata
     3841 agggttggct cactccacgc tttgccataa aaagtgatat tgacgggtag tccggagacg
     3901 aaacccatgg gcaggctgat tgccggatat ccggcaatgg cagcagccga gcttgaagag
     3961 ataccgaaat tatcgccatt cacccaatcg gttttccatg ctggagaacc ggtgggtgcg
     4021 attatcgcgt ccagcctgtg agtgtccatt attttgtcga tcccgttatc ccgtgcacct
     4081 ttcagcat
//
LOCUS       DAOSDR010000291         4077 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrimonas sp. isolate AMR_MDS_1826
            NODE_7748_length_4077_cov_4.123570, whole genome shotgun sequence.
ACCESSION   DAOSDR010000291 DAOSDR010000000
VERSION     DAOSDR010000291.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863355
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrimonas sp. (wastewater metagenome)
  ORGANISM  Petrimonas sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Dysgonomonadaceae; Petrimonas.
REFERENCE   1  (bases 1 to 4077)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4077)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:10:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,720
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 35
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4077
                     /organism="Petrimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7748_length_4077_cov_4.123570"
                     /isolate="AMR_MDS_1826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2023866"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..382)
                     /locus_tag="PLI30_10635"
     CDS             complement(<1..382)
                     /locus_tag="PLI30_10635"
                     /inference="COORDINATES: protein motif:HMM:NF014627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HOI80046.1"
                     /translation="MNLPAKKYLKAFEWRDYLLVVIGLVLYAIGLAGFLIPNKIVTGG
                     LAGVSLLINYSTGVPVWFSILVINCILLIIAWFILGRGFVFKTLFGAFGLTFIIGIAE
                     KYLTHSLLPSADPLMASIIGAICCG"
     gene            complement(428..3181)
                     /gene="leuS"
                     /locus_tag="PLI30_10640"
     CDS             complement(428..3181)
                     /gene="leuS"
                     /locus_tag="PLI30_10640"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005849960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HOI80047.1"
                     /translation="MEYNFGEIEKRWQQYWADREVYRVTEDTTKPKFYVLDMFPYPSG
                     AGLHVGHPLGYIASDIYSRYKRLQGFNVLHPMGYDAYGLPAEQYAIQTGQHPAITTKN
                     NIARYREQLDKIGFSYDWSREVQTCDPEYYKWTQWAFVKMFRSYYCNQAQQARPLDEL
                     IEVFSQQGTTGLDLACTEPMSFTADEWNAKTEKEQQEILMNYRIAYLGDTLVNWCPQL
                     GTVLANDEVSEGLSIRGGYPVEQKKMRQWCLRVSAYAQRLLDGLDDVDWTDSLKETQR
                     NWIGRSVGAVMKFKTPGGVSFDIFTTRADTIFGVTFMVLAPESELVEQLTTPEQRNAV
                     DAYILKTKKRTERERIADKSVSGVFSGSYALHPFTGDLLPIWISDYVLAGYGTGAIMA
                     VPAHDSRDYAFAKHFNLSIVPLIEGCDISEESFDAKEGILMNSGFLNGLPVKEAIVKA
                     IEEVEERNLGFRKVNFRLRDAIFSRQRYWGEPFPVYYKDGMPYTLDEGELPLELPEVD
                     KYLPTESGEPPLGRARNWQTREGYPLELSTMPGFAGSSAYYLRYMDPRNSQALVSKEA
                     NSYWRSVDLYIGGTEHATGHLIYSRFWNKFLFDLGVSCEQEPFRKLVNQGMIQGRSNF
                     VYRIKETNTFVSLNLKDQYDVTPIHVDVNIVHNDVLDVEAFRNWNPEYKNAEFILEDG
                     KYICGWAVEKMSKSMFNVVNPDVIVENYGADTLRLYEMFLGPLELSKPWDTNGIDGVH
                     RFLRRLWNLFHTNGEFLVSDEDPTKEELKSLHKLIKKVSFDIENFSFNTSVSAFMICV
                     NELSQLKCNKKAILSDLLVCLAPFAPHIAEELWHKLGNEQTICDAKYPELNEEYLKEN
                     VFSYAISFNGKSRFVLEFPADASNAEIEKTVLEHPYSQKWLDGKSPRKVIIVPRKIVN
                     IVV"
     gene            complement(3233..>4077)
                     /locus_tag="PLI30_10645"
     CDS             complement(3233..>4077)
                     /locus_tag="PLI30_10645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006799502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HOI80048.1"
                     /translation="FGVALKFFLYKLTIFYLIKKVVGIDRGNFFPVAGAPLSDKINEF
                     MQSIDVHLIYGYGLSETTATVSCFLEKGFTIGTVGKVMPEVEVKIGTNDEILVKGKTV
                     MKGYYKKPVETAAIFTEDGFFRTGDAGRLTGNNEIILTERIKDLYKTSNGKYIAPQMI
                     ETRMTEDKFIDQIAVIGDERKFVSALIVPDFGHLKQYALEHQIPCGSNEELVENKEIH
                     DYMFGRIELLQAQFTSYEKIKKITLLPHPFTMESGELTNTLKLRRKIVLQRYAAEIEK
                     MYAE"
BASE COUNT         1014 a         1050 c          915 g         1098 t
ORIGIN      
        1 caccgcagca gattgcgcct atgatactcg ccatgagtgg atctgcgctg gggagtagcg
       61 aatgtgtcag gtatttctca gcgataccga taatgaaggt cagcccgaat gcgccgaaaa
      121 gtgttttaaa cacaaaacca cgccccaata taaaccacgc gatgatcaac aagatacaat
      181 tgataaccag aatggaaaac cacacgggga cgccggttga gtagttgatg agcaaagaga
      241 caccggccag tccaccggta acgatcttgt tgggaatcag gaaaccggcc agtccgatgg
      301 cgtagagaac cagcccgatt acgaccaaca ggtaatcgcg ccattcaaat gctttaaggt
      361 atttttttgc aggaaggttc attgacattc aatgttttag tcctgaattt ataaatggct
      421 ataagcgtca gacaacgata tttacaattt tccgcggcac aatgatcact tttctcggtg
      481 attttccgtc cagccatttc tgtgaataag gatgttccag caccgttttt tcaatttcag
      541 cgttggatgc atcggccgga aattccagca caaagcgtga ctttccgtta aatgagatgg
      601 cgtaagagaa cacgttttct tttaaatatt cttcgttaag ttcgggatat tttgcgtcgc
      661 aaatggtctg ttcatttccc aacttgtgcc acaactcttc ggcaatgtgc ggagcgaaag
      721 gagccagaca gaccaacaaa tcgctgagga tggctttttt attacatttt agctgagaca
      781 gttcgttgac gcaaatcata aacgccgaca cggatgtgtt gaacgagaaa ttctcaatat
      841 caaacgacac tttcttgatg agtttatgca acgatttcaa ttcctctttt gttgggtctt
      901 cgtcagaaac caaaaactcc ccgttggtgt gaaacaagtt ccaaagcctg cgcaaaaaac
      961 ggtgtacacc gtcgatgccg ttcgtatccc acggtttgga aagctccaga ggccccagga
     1021 acatctcgta caaccgaagc gtatctgcac cgtagttttc cacaatcaca tcgggattca
     1081 ccacgttaaa catcgatttc gacatctttt cgacggccca cccgcaaatg tacttcccgt
     1141 cttccaaaat aaattcagcg ttcttatact cggggttcca gttgcgaaaa gcctccacgt
     1201 ccagaacatc gttatgaaca atgtttacat ccacgtgtat gggcgtgaca tcgtactgat
     1261 ctttaaggtt cagcgagaca aatgtgttgg tctccttaat ccggtacaca aaattgcttc
     1321 gtccctggat cataccctga ttaacgagtt tccggaacgg ctcctgctcg caagagacgc
     1381 ccaggtcgaa caggaattta ttccagaacc ggctataaat caagtgtccc gtcgcatgct
     1441 ctgttccacc gatataaagg tctacgcttc gccaatagct attggcttcc ttcgacacca
     1501 aagcctggct gtttcgcgga tccatgtacc gcaagtagta ggccgatgag cctgcaaatc
     1561 cgggcatcgt actcaactcc aacggatacc cctcccttgt ctgccagttc ctggcacggc
     1621 caagcggggg ttctcccgat tctgttggga ggtatttatc cacttcgggc agttcgagcg
     1681 ggagttcgcc ctcatccagc gtgtagggca tcccgtcttt gtaataaacg gggaacggtt
     1741 cgccccaata acgttgacgc gagaaaatgg catccctcaa ccggaagttc actttccgga
     1801 aacccaggtt gcgctcttcc acctcctcaa tggctttaac aatcgcctct tttaccggaa
     1861 gcccgtttaa aaaaccggaa ttcatcagga taccttcctt ggcatcgaaa ctctcttccg
     1921 atatatcgca gccctcgatc aagggaacaa tggacaagtt aaaatgtttg gcgaaagcgt
     1981 agtcgcggct gtcgtgagcg ggaacagcca taatcgctcc ggttccgtaa ccggccaaca
     2041 cgtaatccga aatccagatt ggtaaaagat ctccggtaaa cggatgtaat gcataggatc
     2101 cggaaaaaac accgctgacc gatttgtcgg caatgcgctc ccgctccgtg cgtttttttg
     2161 tttttagtat gtatgcatct acagcgttgc gctgttcggg ggtggtgagt tgttcaacca
     2221 gttcactttc cggtgcaagc accatgaagg tgactccgaa aatagtatcg gcccgggtgg
     2281 taaaaatatc gaaggagaca ccgccgggtg ttttaaattt catgactgct ccaacagaac
     2341 gcccaatcca gttccgctgt gtttctttca acgagtccgt ccaatccaca tcatcgagcc
     2401 cgtccagtaa ccgttgcgcg taggcggata cacgcagaca ccactgacgc atcttcttct
     2461 gctcaaccgg atatcctccg cggatagaca gcccttcgct cacttcatca ttggccagca
     2521 ccgttcccaa ctgcgggcac cagtttacca gggtatcgcc caggtaggcg atgcggtaat
     2581 tcatcaggat ttcctgttgc tctttttcgg ttttggcatt ccactcatca gcggtgaagc
     2641 tcatcggctc tgtacaagcc aggtccaacc cggtagtgcc ttgttgtgaa aagacctcga
     2701 tcagttcatc caacggcctt gcttgttgtg cctggttgca gtagtaggaa cggaacattt
     2761 ttacaaatgc ccattgcgtc catttataat attcaggatc gcaggtttgc acctcgcggc
     2821 tccaatcgta agaaaatcct attttgtcca actgctcgcg gtagcgggca atgttgtttt
     2881 tggtagtgat ggccggatgc tggccggttt ggatagcata ttgttccgca ggaaggccat
     2941 atgcgtcgta acccataggg tgaagcacgt tgaatccctg cagccgttta tacctcgaat
     3001 aaatatcgga agcgatataa cctagcggat gccccacgtg caacccggct cccgaggggt
     3061 acgggaacat atccagcaca taaaacttgg gtttggttgt atcttctgtt acccggtata
     3121 cctcacggtc ggcccaatac tgctgccacc gtttttctat ttccccaaaa ttatattcca
     3181 ttttttcact tttaaaaaac ggtgcaaaga taatgatttt cccgcctaaa agctattctg
     3241 catacatttt ttcaatttca gcggcatacc gttggagcac gatttttctt ctcagtttta
     3301 acgtattggt cagttcgccc gattccatcg taaaaggatg gggcagcaac gttatttttt
     3361 tgattttttc ataggaggtg aactgagcct gtagtagttc gatccggcca aacatataat
     3421 cgtgaatctc tttattttcc accagctctt cgtttgaccc gcagggaatc tgatgctcga
     3481 gggcgtattg tttcagatgt ccgaagtccg gaacaatcaa cgcgcttaca aattttcgtt
     3541 cgtcgccaat tacggcaatc tgatcgataa acttatcctc ggtcatccgg gtctcaatca
     3601 tctgtggagc aatatatttc ccgtttgagg ttttgtaaag gtcttttatt ctttcggtca
     3661 ggataatctc gttgttcccg gtcagccttc ccgcatcacc ggtcctgaaa aaaccgtctt
     3721 ccgtgaatat cgcggccgtc tcaacgggtt ttttgtaata ccccttcatc accgtcttgc
     3781 ctttcaccag gatctcgtcg tttgtcccga tcttcacctc cacctcgggc atgaccttgc
     3841 caacagttcc aatcgtgaac cctttttcca gaaaacaact aaccgtcgcg gtcgtctcgg
     3901 agaggccgta gccgtatatc agatgtacat cgatcgattg catgaactcg ttaatcttgt
     3961 ccgatagagg agctcctgcc acggggaaaa agtttccccg gtcgataccc accaccttct
     4021 tgatcaggta aaagatagtt agtttataga ggaagaattt gagcgcaaca ccaaacg
//
LOCUS       DAOSDR010000292         4059 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrimonas sp. isolate AMR_MDS_1826
            NODE_7795_length_4059_cov_4.068182, whole genome shotgun sequence.
ACCESSION   DAOSDR010000292 DAOSDR010000000
VERSION     DAOSDR010000292.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863355
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrimonas sp. (wastewater metagenome)
  ORGANISM  Petrimonas sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Dysgonomonadaceae; Petrimonas.
REFERENCE   1  (bases 1 to 4059)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4059)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:10:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,720
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 35
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4059
                     /organism="Petrimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7795_length_4059_cov_4.068182"
                     /isolate="AMR_MDS_1826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2023866"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..212)
                     /locus_tag="PLI30_10650"
     CDS             complement(<1..212)
                     /locus_tag="PLI30_10650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019537729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HOI80049.1"
                     /translation="MTKGTVKGIISNLVIVEVDGPVAQNEIAYINLDGTHLMSEVIKV
                     IGKNVYVQVFESTRGLKVGAGVEFRG"
     gene            complement(268..1116)
                     /locus_tag="PLI30_10655"
     CDS             complement(268..1116)
                     /locus_tag="PLI30_10655"
                     /inference="COORDINATES: protein motif:HMM:NF022415.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2764 family protein"
                     /protein_id="HOI80050.1"
                     /translation="MLFKHQYYCFIAGLPDFSFDSMKLPFTVEEFKRMLDEELKPDDK
                     RLLSKYFLKYDNDNLLYLLKNKDAELNPMGSISREEIQETIGRIKEDLPVKNRKVPDF
                     HEKFIRTWLDEEVQAENKLWDDLMASLYMDYGTEVKNSLMSRWFELNLNIGNLLSAIY
                     ARKYGLDVARVVVGHNPVAQLIRENANARDFGLSQELEIFDAVVRLSEETDIYERERK
                     MDKFRWDWLEENTVFDYFNIEYIFAYLCKLQMLERWVKLNAEEGERVFRELIAGLKGD
                     VKMPEE"
     gene            complement(1257..1844)
                     /locus_tag="PLI30_10660"
     CDS             complement(1257..1844)
                     /locus_tag="PLI30_10660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008779777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI80051.1"
                     /translation="MDKIQEITSKLYAEGIEKGKEEANKIVAEAKVLRDQIVEEAKKE
                     AEQIVVSAKKETHEWKSNTESELKLFASQAVEALKSEITNLVTGKLSASNVKAALEDK
                     TFMQKLITELVQNWSKNETLTIGVENSEELKQYISENAKYLLDKGLKIESVNGLKTSF
                     ILSPEDGSYRVKFGEEEFIEYFREFLRPQIQKLLF"
     gene            2026..3324
                     /locus_tag="PLI30_10665"
     CDS             2026..3324
                     /locus_tag="PLI30_10665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016268655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HOI80052.1"
                     /translation="MDKKIFRLAIPNIITNITVPLLGMIDIAIAGHLGSAVYIGAIAL
                     GANIFNMIYWNFGFIRMSSSGFAAQAYGARDFTEAMNVLIRSLIVGVGIGLLIVLLQY
                     PIGKFALSLIKSGSESKAAVETYFRICVWSAPAVLGTYAFKGFFIGMQNAKTPMVIAI
                     LNNVLNIVLSMLFVFGFGMKVAGIALGTMLSQVITLVVCVLMWLKFYGRLRKYIVRSS
                     VLETAAIRSYFRVNGDVFVRTFLLTLVTTFFTFVSSGMGDMILAANALLMQFFMLFSY
                     FMDGFAYAGEALTGRYIGADNKVLLAGMLRRLFFWGFIVSAVSVMIYLFFPDPILKML
                     TNDKAVIETTKSFLFWTVLIPVTGFTAFLWDGVFIGATASKEMRNAMVFSAVVFFACY
                     YIAVPVLGNNGLWLAFILYLSVRGILQTVWAKKALKMAQS"
     gene            3342..>4059
                     /locus_tag="PLI30_10670"
     CDS             3342..>4059
                     /locus_tag="PLI30_10670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI80053.1"
                     /translation="MKQFESITELKRFLTVPYVEEIAVQSLRLTEIEPLMLNIRFSRC
                     LFLGCSMSDDLLHHLLPGNFIFPLLDVPFNTYPSRLYDTDSLYAGFNRHKPKTYLKTP
                     DKVVYDYYRESRKNLSIKDTLAQRLHDHSITDSLHEYIASFDERKLVAIMGGHGILRT
                     EHIYRQVVLLSKSLTEQGYLMLSGGGPGAMEATHLGAWMAGRGDNECLRAVGILSAAP
                     RYSDEGWLSSAFEVMERFPDP"
BASE COUNT          952 a          942 c          885 g         1280 t
ORIGIN      
        1 tgtccccgaa actccacccc ggcgcctacc ttaagaccgc gcgtgctttc aaaaacctgg
       61 acatacacgt tttttccgat aaccttaatg acttccgaca tcagatgcgt accgtcaaga
      121 ttgatatagg caatttcgtt ctgcgcaaca gggccatcta cctccacaat taccaggttg
      181 gaaatgatcc cctttactgt tccttttgtc atattataat tctttgttat ttgcttcgtg
      241 ttatgcaatg gaaattcaac ttctttttta ttcctcaggc atttttacat cgccttttaa
      301 tccggcgatc agttcacgga aaacacgttc accttcttcg gcattcagtt ttacccagcg
      361 ttccagcatc tggagcttgc acaggtaggc aaaaatatat tctatgttga aataatcgaa
      421 aaccgtgttt tcttccagcc aatcccaacg gaatttgtcc attttccttt cgcgttcgta
      481 tatatccgtc tcttcggaga gccgcacgac agcatcaaaa atctccagtt cctgactcaa
      541 cccgaaatcg cgggcattcg cgttctcacg gatcagctga gctaccgggt tatggccaac
      601 gaccacccgg gccacatcca atccatactt tcgggcataa attgccgaca acaggttgcc
      661 tatattcaga ttgagctcaa accatcgcga catgagcgaa tttttcacct ctgtgccgta
      721 atccatatac aaagaggcca tcaggtcgtc ccacaatttg ttttctgcct gaacctcttc
      781 gtccagccat gttcggataa atttttcgtg aaaatccgga accttcctgt tttttaccgg
      841 taagtcctct ttgattcttc cgatcgtttc ctgaatctct tctcgggaaa tactgcccat
      901 cggattcagt tcggcatctt tgtttttcaa caagtacagt aggttgtcat tgtcgtactt
      961 caggaaatat ttactcagca accgtttgtc gtccggtttc aattcttcgt cgagcattcg
     1021 tttaaactct tccacggtaa acggaagctt catgctgtcg aaagaaaaat cgggcaaccc
     1081 tgctataaaa cagtaatatt gatgtttgaa aagcatatgt ttcacagttt atttacctgc
     1141 gttgtaagtt ggcttcgccg taatttgcgt tttgtatact attgagagtg acttaagccg
     1201 tttaacgaaa caacggatta caatagaaca ggtgttgtat tacagctgaa taccttttaa
     1261 aaaagcagtt tttgtatttg cgggcgaagg aattcacgga aatattctat aaactcttct
     1321 tcgccgaatt ttacccggta agaaccgtct tcgggggata agataaagct ggttttcaat
     1381 ccattcacgg attcaatctt caggcctttg tcaagcaggt atttagcgtt ttcggaaatg
     1441 tattgcttca attcctcgga attttctact ccgattgtta atgtttcgtt cttcgaccaa
     1501 ttttgcacaa gttcagtgat cagtttttgc atgaacgttt tgtcttccaa cgctgctttc
     1561 acgttggatg ccgataactt accggtcacc aggttggtga tttcgctctt cagcgcctcg
     1621 accgcctgag atgcgaaaag cttaagttca gactccgtat tgcttttcca ttcgtgggtt
     1681 tctttctttg ctgacacaac gatctgttcg gcctcttttt ttgcctcctc cactatctga
     1741 tcacgcaata cttttgcttc ggcaacaatt ttattggcct cttccttacc tttttctatt
     1801 ccttccgcgt agagtttaga ggtgatttcc tgaattttgt ccataaattt tttctgttat
     1861 tgagcaacaa ggttaatcgc ttattggcta tcaaaatttt atagaacaaa gatataaaaa
     1921 aggaagataa tcatcacaaa atattttctt ttattttttg tggatctttt tttctttcaa
     1981 aatagttttt tacccgtgtc aatgttttgt attatttttg cagccatgga taagaaaatc
     2041 tttcggcttg ccatacccaa tataatcacc aacattactg ttccgctgct tgggatgatc
     2101 gatatcgcca ttgcggggca tttgggttcg gcagtatata taggtgccat agctctcggc
     2161 gccaatatct tcaacatgat ttactggaat tttgggttta tccggatgag ctccagcgga
     2221 tttgccgcgc aggcctatgg agcccgcgat tttaccgaag ccatgaatgt gttgatacgt
     2281 tcgcttatcg tgggtgtggg aatcgggctg ctgattgtct tgttgcaata ccccatcggg
     2341 aagtttgcct taagcctgat aaagtccggt tccgaaagta aagcagctgt tgaaacctat
     2401 ttccgtattt gtgtctggag tgccccggcc gtactcggga cgtatgcttt taaaggtttt
     2461 ttcatcggaa tgcagaatgc taaaacacca atggtaattg ccattctcaa caacgtgctg
     2521 aatattgtcc tgagcatgtt gtttgttttc ggatttggca tgaaggtggc aggaattgcc
     2581 cttgggacaa tgttatcgca ggtgataaca cttgtcgttt gtgtgctgat gtggctgaaa
     2641 ttttacggcc ggctgagaaa atacattgtc aggtcttctg ttttggaaac cgctgccatc
     2701 cggtcttatt tccgggtaaa cggcgatgtt tttgtacgca cgttcctgtt gacgctggtt
     2761 accacgtttt tcacgtttgt gtcatcgggt atgggagaca tgattttagc tgccaacgcc
     2821 ctgttgatgc agtttttcat gcttttctcc tactttatgg atggatttgc atacgcgggg
     2881 gaagcattaa cgggaagata cataggggca gataacaagg tgctgcttgc cgggatgcta
     2941 agaagattgt ttttctgggg atttatcgtg agtgccgtat cggtaatgat ttacctgttc
     3001 ttccccgacc cgattttgaa aatgctcacc aacgataaag cggtcattga aaccaccaaa
     3061 tcgttccttt tctggacagt gcttatcccg gtaaccggtt ttactgcttt tctctgggac
     3121 ggtgttttca ttggggcaac agcctcgaaa gagatgcgca acgctatggt gttctctgct
     3181 gtcgttttct ttgcctgtta ttatatcgcc gtgccggtac tcggaaacaa cggcctgtgg
     3241 ctggctttta ttttgtattt gtccgttcgg ggaattttac aaaccgtatg ggcgaaaaag
     3301 gcgttaaaaa tggcacaatc ctaaacctgt aattcaaaga aatgaaacaa ttcgaatcca
     3361 taaccgaact gaaacgtttt ttaacggttc cttacgtaga agagattgcc gttcaatcgc
     3421 ttcgcctgac cgagattgaa ccgctaatgc tcaatatccg tttttccagg tgtttgtttc
     3481 tgggttgttc gatgtctgac gacctgttgc accatttact gcccgggaat tttatttttc
     3541 ccctcctgga tgttccgttc aatacctatc cgagccggtt gtacgacaca gattcgcttt
     3601 atgccggttt taaccgccac aagcctaaaa cgtacctgaa gactcctgat aaggttgttt
     3661 acgattatta ccgggagtcc cggaaaaacc tgtcgataaa ggatacgctg gcgcaacgtt
     3721 tgcacgacca ctccatcacc gattcgctgc acgagtacat cgccagtttt gatgaacgca
     3781 agctcgtggc aataatgggc ggacatggca tcctgcgaac cgaacacatc taccggcaag
     3841 tggtcttgct gtcgaaatca ctcacggagc agggttacct gatgctttcc ggaggcggcc
     3901 ccggggcaat ggaagccacg cacctgggag cgtggatggc cggaaggggc gacaacgaat
     3961 gtcttcgggc cgtcggcatc ttgagtgcgg ccccccgtta ctccgatgag ggatggcttt
     4021 cttcggcatt cgaggtaatg gaacgtttcc ccgacccgc
//
LOCUS       DAOSDR010000293         4057 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrimonas sp. isolate AMR_MDS_1826
            NODE_7804_length_4057_cov_3.315592, whole genome shotgun sequence.
ACCESSION   DAOSDR010000293 DAOSDR010000000
VERSION     DAOSDR010000293.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863355
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrimonas sp. (wastewater metagenome)
  ORGANISM  Petrimonas sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Dysgonomonadaceae; Petrimonas.
REFERENCE   1  (bases 1 to 4057)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4057)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:10:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,720
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 35
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4057
                     /organism="Petrimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7804_length_4057_cov_3.315592"
                     /isolate="AMR_MDS_1826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2023866"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2949)
                     /locus_tag="PLI30_10675"
     CDS             complement(<1..2949)
                     /locus_tag="PLI30_10675"
                     /inference="COORDINATES: protein motif:HMM:NF039534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HYR domain-containing protein"
                     /protein_id="HOI80054.1"
                     /translation="MTALLAGQTYPVEVVAKTNNLYKEYVKIWFDFNGNGVLSDPNEM
                     VFAAGNSFNGTYVYAGNITIPSDAFNGEVYIRVVMVYNNSPALCGSYQYGTTMDIKAT
                     IMGGKVPRKLTVKVGEGEELRGRVESTPSGINTDKGLNSANFADSSVVTLTAFPAANA
                     EFVNWEGDASGTNPVLTVNMDAAKDITARFALLPPPPSPTAITTASPAICEGETALLT
                     VENAVGTVHWYTGSCGGDLVVTGNPISVKPSSSTVYYARNYTAGGFSAECASIEITVA
                     PKGVGGTISGENALICNGKSTGQMTLTGNVGVVQRWEKRVDGGEWQPITHTGEDYSET
                     PSSVGAWDYRAVIKNGICSEVNSALWSVHVDGSAPIMTTPAADLTVCDQAGSNDLLNT
                     WLANHGGANATDDYHSVTWSNNFDADNWTAICKNVKSQQVVFTATDDCGYQSTTSAVF
                     TVEDTAAVSIVCKDITLYLDASGSVSVPIDQMADVAVESCGFVSISVSPDAFYCSDVG
                     ENTVTLSVRDFCNNLTTCSAVVTVRDTLAPVFSKPVVPVLPKTISQLGHKSTYSNVRL
                     NGGSNLLTVSPGAQVTLTANWSAAYDGGGGCGGCITQHHIGLGNIFSECHAMSSGTGT
                     INKTFTAPVVPGIYYINQQATWWFYCGQFARPVHNNDHNTAIAVLVVADESFGACIPD
                     FTVKTDPGSRRAVVNYFPPVAVDNCSSTTTQTEGLPSGSEFEFGTTLNTFVATDDAGN
                     TSSCSFVVTVVDKEPPVALCKDVTVYLDEAGQASIDHHAVDNGSNDLFGIYSLVTSKV
                     NFTCSDECSGSEESCPEVTLTVTDMNGNVSTCTSQVTVLDTISPKALSKDITVFLDET
                     GNVLITAADVDGGSSDACGIASMSVSPNTFTCEEVGENSVVLTVTDNNGNTSTATAVV
                     TVEDKIAPEAVGKNITIQLDASGAALIVPPHIEAGSSDACGIASISVSPNVFTCEEVG
                     ENSVVLTVTDN"
     gene            complement(3166..3606)
                     /locus_tag="PLI30_10680"
     CDS             complement(3166..3606)
                     /locus_tag="PLI30_10680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI80055.1"
                     /translation="MRTKFRLAFFLLGLLGSISASSHAQAVFDPRSSEIIMKFEDFDF
                     AGKFAALVQKDEESNYFLVDVTKLPTQFELMYFLKLTREVGYMALTDHGLTKQKAWFQ
                     VNKKYQEAEILKMFSELKNKVEEKATLFSETEKEQWIKDSVSGK"
BASE COUNT         1139 a         1034 c          851 g         1033 t
ORIGIN      
        1 gttgtcggtc acggtcagca cgacgctgtt ctcgcccacc tcttcgcagg taaacacgtt
       61 gggagaaaca ctgatcgaag caattccgca ggcgtcggac gaaccggctt caatatgggg
      121 aggaacaatc aaagccgcac cactggcatc gagctgaatg gtgatatttt taccaacagc
      181 ctccggcgcg atcttgtctt cgaccgtcac cacagcggtt gcggtgctgg tatttccatt
      241 gttgtcggtc acggtcagca cgacgctgtt ctcgcccacc tcttcgcagg taaacgtgtt
      301 gggagaaaca ctcatcgaag caattccgca ggcgtcagac gaaccgccgt caacgtcggc
      361 agccgtaatc aagacgtttc ctgtctcatc caggaaaacg gttatatctt tagacaacgc
      421 tttcggggaa atggtatcca gaacggtcac ctgcgaagta caggtagaga cgttgccgtt
      481 catgtcggtc acggtcagcg tcacttccgg acaagactct tcacttccgg aacattcgtc
      541 ggaacaggtg aaatttacct tactggtcac cagagaatag attccaaaca gatcattgga
      601 gccgttgtcg actgcgtgat gatcaatcga agcctgaccg gcctcatcca ggtaaacggt
      661 gacatccttg cacaaggcaa caggaggttc tttgtcgacg actgttacga caaaactaca
      721 cgagcttgta tttccggcat catccgttgc gacaaaggta ttcagcgttg ttccaaattc
      781 aaactcactt ccgctgggta aaccttcggt ttgagtggta gttgaactgc aattgtccac
      841 cgcaacaggt ggaaaatagt tcaccactgc gcgccgtgac cccggatcgg tttttaccgt
      901 aaaatcggga atacaagccc caaaactttc atccgcaacc accaaaacag caatagcagt
      961 attatggtcg ttgttgtgaa ccggacgtgc aaattgtccg caataaaacc accaggttgc
     1021 ctgctgattg atataataaa ttccgggaac aaccggtgcg gtaaatgttt tattgattgt
     1081 tcctgttcca gaactcatgg cgtggcattc cgaaaagata ttccccaacc caatatgatg
     1141 ttgagtaatg catccgccac aacctccacc tccatcataa gcagcggacc aattggcagt
     1201 caaggttacc tgcgcaccgg gggacaccgt caataagttg gacccgccgt ttaggcggac
     1261 gttgctgtag gttgatttat gccccaactg ggagatggtt ttaggcaaaa ccggcacgac
     1321 gggtttacta aaaaccggag ccagggtatc ccgtacggtt acaaccgcgg aacaggtcgt
     1381 cagattattg cagaaatccc tgacagacag cgttaccgtg ttttctccaa catcagaaca
     1441 ataaaaagca tccggagaca cacttattga gacgaacccg cagctttcaa cagccacatc
     1501 ggccatctgg tcgataggaa ctgaaacgct accactggca tcgagataaa gggttatatc
     1561 tttacagaca atgcttactg cagcggtatc ttccaccgta aaaacagctg aggtagtcga
     1621 ctggtagcca caatcatcgg tggcggtaaa aaccacctgc tgggatttga catttttgca
     1681 gatcgccgtc cagttgtcgg catcgaagtt atttgaccag gtgacactgt ggtaatcgtc
     1741 ggtagcattt gcgcctccgt gattggccaa ccaggtattg agcaggtcgt tactcccagc
     1801 ttgatcgcaa accgttaaat cagcggctgg ggttgtcata atgggagctg aaccatccac
     1861 atgaaccgac cacagtgcag agttcacttc agaacaaata ccatttttga tcactgcccg
     1921 gtaatcccag gctccaaccg aagaaggagt ttcgctgtaa tcttctccgg tatgggttat
     1981 cggttgccat tcgccgccgt caactctttt ttcccatcgc tggacgaccc ccacattccc
     2041 ggtcaacgtc atttgtcctg tagacttacc attgcagatc aaggcatttt ccccgctaat
     2101 ggtaccacca actcctttcg gagccaccgt aatttcaatt gaagcacact ctgcgctaaa
     2161 tccgcctgca gtataatttc gtgcataata gaccgtggat gaagagggct ttacgctaat
     2221 cgggtttccg gttacaacca aatccccacc acaactaccc gtgtaccaat ggactgtccc
     2281 cacggcattt tctactgtta aaagcgcggt ttctccctca caaatagccg gcgaagcagt
     2341 tgttatggca gtgggagacg gcggcggtgg caggagggca aaccgggccg taatatcttt
     2401 tgcagcatcc atatttacag tcaaaaccgg attggttccc gaagcatcgc cttcccagtt
     2461 tacaaattca gcattagctg ccggaaaagc ggtaagtgta acgaccgaac tatcggcaaa
     2521 attggcagaa tttagtccct tgtcagtgtt aattccagaa ggggtgctct ccacacgtcc
     2581 tctcagttct tccccctcac caaccttaac cgtgagtttt ctgggcactt ttcctcccat
     2641 aatggtcgcc ttgatatcca ttgtggtacc atattgatag gaaccacaca aagctggaga
     2701 attattataa accattacca cccgaatata cacctctcca ttaaaggcat ccgacgggat
     2761 cgtaatattc cctgcataca cataggtgcc attaaaagaa ttgcccgctg caaagaccat
     2821 ttcattggga tctgagagca caccattccc gttaaaatca aaccagattt taacatattc
     2881 cttatacaaa ttgttggttt ttgcaactac ctcaacggga taagtttggc cggctaacaa
     2941 agcagtcaca ctggaaccgg tataatcaaa atatccgggc cccgagctaa aacctgttgc
     3001 atcaggggcg ccagccaacg ggactccgtt aatgcgaacc tcggaaatat actcacaaca
     3061 ataagtagtc ggaaaagagg gacataatgg agattgtgcc attaaactga ccacgacccc
     3121 ataaacaaaa catattagaa gtacagcttt tttcatatct gatttttatt ttcctgaaac
     3181 actatctttt atccactgtt ctttttcagt ttcactaaac aaggttgctt tttcctcaac
     3241 cttatttttc agctcagaaa acattttcag gatttcagcc tcctgatatt ttttgtttac
     3301 ctggaaccat gccttttgtt tggtcagccc atgatccgta agcgccatat aaccaacctc
     3361 ccgggtcagc ttcagaaaat acatcaactc aaactgggtt ggcagtttgg taacatctac
     3421 caggaaataa ttactttcct catctttttg gaccagcgcc gcaaattttc cggcaaagtc
     3481 gaaatcctca aatttcatga ttatttcaga agagcgcgga tcaaataccg cttgtgcatg
     3541 gctgctggca gaaatagatc caagcagccc caatagaaaa aaagcaagtc gaaattttgt
     3601 tctcattttg caatagtcta gataaaacaa attatgtata aacattaaaa aacgttgacg
     3661 acatccctta cagtcaactc aataactgca aaaccaacaa aacaaatttc tgtctcctat
     3721 cgtgaaataa tcaaacgatt agttccgtcc ctgctactaa tctgtatcta tgatttgtaa
     3781 tcatcaaaaa taaaaattat caaaaccaga caacctgata aaaaagtttt tttcaaaaaa
     3841 attatcggcc gtatccccaa tctttacgaa gtaatccaaa taaaaaaggg taacatttgc
     3901 ttacaaacat tacccttttt aaataattca atcgtggcaa agcaattcac ttatgcctgc
     3961 cagccgggaa actattgtgc ctcgagcacc agtttttgta cggtcggaat gccgtcaacg
     4021 gtgatcttaa ccagataaag gcctgtcaca tacggcg
//
LOCUS       DAOSDR010000294         4053 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrimonas sp. isolate AMR_MDS_1826
            NODE_7811_length_4053_cov_3.872186, whole genome shotgun sequence.
ACCESSION   DAOSDR010000294 DAOSDR010000000
VERSION     DAOSDR010000294.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863355
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrimonas sp. (wastewater metagenome)
  ORGANISM  Petrimonas sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Dysgonomonadaceae; Petrimonas.
REFERENCE   1  (bases 1 to 4053)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4053)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:10:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,720
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 35
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4053
                     /organism="Petrimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7811_length_4053_cov_3.872186"
                     /isolate="AMR_MDS_1826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2023866"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(163..2568)
                     /locus_tag="PLI30_10685"
     CDS             complement(163..2568)
                     /locus_tag="PLI30_10685"
                     /inference="COORDINATES: protein motif:HMM:NF018234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HOI80056.1"
                     /translation="MINNKRLKVMRMQFVFLLLCIAGSTFSKTSNVDPREKRGIRIAT
                     FDVDATPPIGSRLTYDPMINSGEQTLRAKGIVLFGEGDPIVLCSVDWIGIANESQDAF
                     KEAMAEAAGTIPNRVVVHTVHQHDAPICDFTAEKILKENNIPAGCFDGTFARELIQRL
                     QSSIRLSLKDVKEVTEIGIGSAEIHQVASNRRVYKKEGRIVTMRGSSSKDSLLRSMPE
                     GLIDPDVSLVSFWNENTPLAVLSFYATHPQSYYLTKTTNPDFPGIARFLRQLAVPDAL
                     HVHFNGAGGNVAAGKYNDGSHETRFILAKRMADGMERAWNNTQKFSVQSEDLGWDTEN
                     LLLPYRDKIKEIETTMNQMDSRLLANNMGRLGWYKRRVAGKGIEIACLRIHNARLLFM
                     PGELFVEYQLAAKKMAPDHFVAMAAYGDYGPFYIGTEAAYAEGGYEIESSPVTAESEK
                     LILTKIKRLLDRNKANQPRELLTYKDRSGQIFPVTNKKEWEKKREEVLNNMQLVMGKV
                     PPRQKKKLHVIYTDSAVYEKYVRYSINFEAAPSEKVFAYLYRPKNKSMLSPAMLALHG
                     TGIEGKKIIDGVTSIKNRAYAKELAERGYVVISPDYPSMGELENYDFANDRYDSGTMK
                     GIFNHISCVDILQSLPYVDKKRIGVIGHSLGGHNALFVAAFDPRIKVIVSSCGWTLMD
                     YYHPGEQVTAGYGGRLGPWAQERYMPFIRDKYNLDVEKIPFDFDEIIASLAPRPFYSN
                     SPVSDLNFSIEGVRKGISHVREVYRFLDSEDHLKIVYPQASHDFPSDIREDAYRFIDK
                     YLK"
     gene            complement(2630..>4053)
                     /locus_tag="PLI30_10690"
     CDS             complement(2630..>4053)
                     /locus_tag="PLI30_10690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI80057.1"
                     /translation="YNKIKSLSFEKINIMKKNYLFYSAILLLALFFTQCVQQNKQANL
                     SEISSGNQTDTMIAKRYFSGFEDAHDTYNAITEASDGKIYYILSSTRHDVGGQMYSYD
                     PQLDKTEFIADLSEALGEKEQNYIAQGKSHTEFYEHDNKLYFSTHVGYYEMIDGAEHL
                     PKNAPEGYKLYPGGHFLYYDMSTKKLVSLGLAPDGEGILTMTMDKERGHLYGITWPKG
                     YFIDYDLNTNKMNNLGLVSEQGENGVIGKDYRVLCRSMFVDPRDGNVYYSVAEGDIYY
                     YHPSDPQLKKMDGVDLRLDYFGKFDASDAGSMGYNWRKIYWYEPENKAYGVHGNSGYL
                     FTFDPLNKKVEIVDRITSEPSRKSGMSDQFSYGYLGFKIKDGVIYYLTGAPIYKDGVR
                     VAGLDKINMGAAKGLEHLHVVTYNIPEKKYKDLGPVFYEDGSFPTYVNSIAMGSQNDI
                     YTLARFENNGKVIEDLVRIDIKR"
BASE COUNT         1087 a          934 c          791 g         1241 t
ORIGIN      
        1 aaatagcttc ccaaaggcta taaacacttt aagcaaaaaa aagtacttga tttctcggtg
       61 aatagagaga aaagagatat ctgctatcaa gactaatttt cgaagacatt tgtgtcaggt
      121 ataccctcag ttattaggta taaattccca tttacaacta acttatttta aatatttgtc
      181 gataaatcgg tacgcatctt cccttatatc agagggaaaa tcgtgacttg cttgagggta
      241 aacgattttt aaatgatcct cactatccaa aaaacgataa acctccctga cgtgactgat
      301 gccttttctg acgccttcaa tagaaaaatt gaggtcagat acaggagaat ttgaataaaa
      361 aggcctgggg gcaagtgatg caatgatttc atcgaaatca aaagggattt tttcaacatc
      421 cagattgtat ttatccctta taaagggcat atatcgctct tgagcccacg gacccagacg
      481 gccaccgtat ccggcagtta cttgttcacc ggggtggtag tagtccatca atgtccatcc
      541 acaactggag acgattactt ttatccgggg atcaaaggca gcaacaaaca gggcattatg
      601 accacccagt gagtggccta taaccccaat tctcttcttg tcaacgtaag gcaggctttg
      661 cagtatatcg acacaagaga tatggttgaa gatccctttc attgttcccg aatcatagcg
      721 gtcattagca aagtcgtaat tttccagctc acccatactc ggatagtctg gtgaaataac
      781 tacatatcct ctttctgcaa gctccttggc gtaagccctg tttttaatac tggtaacgcc
      841 atctattatc ttcttgcctt caatgccggt tccgtgaaga gcaagcatag caggagataa
      901 catcgattta ttttttggcc ggtagagata ggcgaacact ttttcagagg gtgctgcctc
      961 aaagttgata ctataacgta catatttttc atacacagca gagtcagtat agatcacatg
     1021 caattttttc ttctgccgtg gcgggacttt tcccatgaca agttgcatat tgtttagtac
     1081 ctcctctctt tttttctccc attctttttt atttgtgaca ggaaaaattt gaccgctacg
     1141 atctttgtag gtcaacaact cccggggttg gttggctttg tttcggtcta aaagccgctt
     1201 aatcttcgtc aggatcaact tttccgattc tgcggttaca ggactggatt caatctcata
     1261 ccctccttca gcgtaggctg cctccgttcc gatataaaat gggccgtaat caccgtaagc
     1321 tgccatcgca acaaaatgat ccggcgccat ctttttggct gccagctgat actccacaaa
     1381 gagttcaccc ggcataaaga gcaaacgggc gttatgaata cgcaaacagg cgatttctat
     1441 cccctttcct gcaaccctcc ttttatacca tcccaaacgc cccatattgt tggcaagcaa
     1501 acggctatcc atttgattca tggtcgtctc tatctctttg attttatccc gatagggtag
     1561 caacaggttt tcggtatccc aacccagatc ttctgattga acactgaact tttgggtatt
     1621 attccacgcg cgctccatgc cgtctgccat tctctttgcg aggatgaaac gggtttcatg
     1681 gctgccatcg ttatactttc cggctgcaac attacctcca gccccgttga aatgaacatg
     1741 caaggcatcc ggtacagcca attgacgcaa aaaacgggca ataccgggaa aatcagggtt
     1801 ggttgttttg gtcaggtagt aactctgcgg atgggttgca taaaaactca atacagccag
     1861 cggagtattc tcattccaga aacttacaag cgacacatcc ggatctatta atccctctgg
     1921 catggaacgg aggagagaat ccttactcga agagccacgc atcgtaacaa tccgtccctc
     1981 ttttttgtaa actcttctgt ttgaagctac ctgatgtatc tccgcactac cgatacctat
     2041 ctctgtcacc tccttcacgt ctttgagtga taaacggatt gatgactgaa gcctttgaat
     2101 caactccctt gcaaaagtgc cgtcaaaaca gcctgctgga atgttgtttt cttttaaaat
     2161 tttttctgca gtgaaatcac agatgggagc atcgtgctga tgaactgtat gtaccaccac
     2221 ccggttagga atagtacccg cagcttcggc catggcttcc ttgaaggcat cctgtgactc
     2281 gttagcaata cctatccagt caacagaaca caacacgata ggatcacctt cgccaaataa
     2341 aacaatgccc tttgccctca atgtttgttc tccggagttt atcatcggat cgtaagtgag
     2401 tctgctccct ataggtggag tagcatccac atcaaaagta gcaatcctta ttcctcgttt
     2461 ctcccgggga tcaacatttg aagtctttga aaatgttgat cccgcaatac aaagaagaag
     2521 aaaaacaaat tgcattctca ttactttcag ccgtttgttg ttaatcatcc catctttttt
     2581 ttataaattc aacttctgtg agtttcctca gcctgtcagt aacagtcttt tatcgtttta
     2641 tatcgattct taccagatct tcaatgactt ttccgttgtt ttcgaatctg gccagtgtgt
     2701 aaatgtcgtt ttggctcccc attgcgattg agttgacata agtagggaaa gaaccatctt
     2761 cataaaagac cggtcccagg tctttgtatt tcttttcggg aatattgtat gttaccacat
     2821 gcaggtgttc caggccttta gctgcgccca tgttaatttt atcaagaccg gcaactctaa
     2881 cgccatcctt gtagatagga gctccggtaa gataatagat aacgccgtct ttaattttga
     2941 atccaagata accatagctg aactggtcgg acattccact ttttctggac ggttccgaag
     3001 taattctatc cacaatctcc actttcttgt taaggggatc aaaggtgaaa agataaccgg
     3061 agttgccatg cacgccgtat gctttatttt ccggttcgta ccagtatatt tttctccagt
     3121 tatatcccat actgccggca tcactggcat caaatttccc aaaataatcg agtctcaaat
     3181 ccactccatc catttttttc agttgaggat cggaaggatg gtaataatag atgtctcctt
     3241 cggcaacaga ataatagaca ttgccatccc ttggatctac gaacatagag cggcagagca
     3301 ccctgtagtc tttcccgatt acaccgtttt ctccctgttc actaacaagg cctaaattgt
     3361 tcatcttgtt ggtattgagg tcgtagtcga taaaatatcc tttaggccag gtaataccat
     3421 aaaggtgtcc cctctctttg tccatggtca tggtgagaat cccctctccg tcgggggcaa
     3481 gtccgagact taccagtttt ttggtgctca tgtcataata gagaaaatga ccacccgggt
     3541 aaagcttgta gccttccggt gcatttttcg gcaaatgttc cgcaccgtca atcatttcgt
     3601 agtaacctac atgagttgag aagtacagtt tattgtcatg ttcgtaaaat tcggtatgac
     3661 tttttccctg cgcaatgtag ttttgttctt tctctcccaa tgcttcagat aggtcggcta
     3721 taaactctgt cttatctagc tgaggatcat atgaatacat ctgaccgccc acatcgtgac
     3781 gagtagagga gaggatatag taaatctttc cgtcgcttgc ttccgtaatt gcattatagg
     3841 tatcgtgtgc atcctcaaaa cctgaaaaat accgtttggc aatcattgta tcggtttgat
     3901 tcccagagga aatttcactt agatttgctt gcttgttttg ttgaacgcat tgagtaaaaa
     3961 acaacgctag taatagtata gcactgtaaa acaaataatt tttcttcatg atgttaatct
     4021 tttcaaatga taatgatttt attttgttgt aat
//
LOCUS       DAOSDR010000295         4040 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrimonas sp. isolate AMR_MDS_1826
            NODE_7845_length_4040_cov_4.556085, whole genome shotgun sequence.
ACCESSION   DAOSDR010000295 DAOSDR010000000
VERSION     DAOSDR010000295.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863355
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrimonas sp. (wastewater metagenome)
  ORGANISM  Petrimonas sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Dysgonomonadaceae; Petrimonas.
REFERENCE   1  (bases 1 to 4040)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4040)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:10:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,720
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 35
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4040
                     /organism="Petrimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7845_length_4040_cov_4.556085"
                     /isolate="AMR_MDS_1826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2023866"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            64..>560
                     /locus_tag="PLI30_10695"
     CDS             64..>560
                     /locus_tag="PLI30_10695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009017033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HOI80058.1"
                     /translation="MEISSKYNPAEVEDKWYSYWMEHKLFRSKPDEREPYTIVIPPPN
                     VTGILHMGHMLNNTIQDILIRRARMEGKNACWVPGTDHASIATEAKVVAKLAAEGIKK
                     SDLTREEFLSHAWEWTHKHGGMILEQLKKLGASCDWDRTCFTMDEKRSESVINVFVDL
                     YNKGLV"
     assembly_gap    561..570
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <571..2685
                     /locus_tag="PLI30_10700"
     CDS             <571..2685
                     /locus_tag="PLI30_10700"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009017033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HOI80059.1"
                     /translation="GVRMVNWDPQALTALSDEEVVHKEVNGKLYYLRYKIEGEETYAV
                     VATTRPETIMGDTAMCIHPNNPKTAHLKGKKVIVPLIGRVIPVIEDEYVDMEFGTGCL
                     KVTPAHDVNDYVLGEKYNLPSIDIFNDNGTLNENGAMYAGKDRFEVRKLIEKDLENAG
                     LIEKTEPYTNKVGFSERTNVAIEPKLSMQWFLKMEDIAKPALDAVENDTIKFHPVKYK
                     NTYRHWMENIKDWCISRQLWWGHQIPAYYLPKGGVVVAVTKEEAYQKALQTVDYPLTI
                     DDLRQDEDCLDTWFSSWLWPISVFDGINQPDNPDISYYYPTNDLVTGPDIIFFWVARM
                     IMAGYEYRQSLCFSNVYFTGIVRDIQGRKMSKQLGNSPDPIELMARHGADGVRMGMLL
                     ASSAGNDLLFDESLCEQGRNFNNKIWNAFRLVKGWEVNDTNEQPETAKIAINWFGNVL
                     SKTIVELDDLFSKYRLSEALMMVYKLFWDEFSSWYLELVKPAYRQPIDRATYQATLAF
                     FDSLLRLLHPFMPFITEELWQALNERKPGESITIAQQPKANRVDEKLIADFEITKQII
                     AGIRTIRLQKNIPNKDELTLQVIGNHDSRFDTAVAKMANLSSIENLSEKPAVTIGFLV
                     GTTEFIVPMSDKMDVEAELKKMKAEREYYAGFLQSVMKKLGNERFVQNAKPEIVENER
                     KKQADAESKIALLEESIKALEQ"
     gene            2692..3549
                     /locus_tag="PLI30_10705"
     CDS             2692..3549
                     /locus_tag="PLI30_10705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019538120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HOI80060.1"
                     /translation="MQTILSHISQEIGEGIASYRSIPGGDISSAYQLQTETKKYFLKV
                     NTNPFALAMFHAEQKGLQTIEKTKTIAIPHVYVVGSLEGKSFLLMDFVEAKRPDANDF
                     RQFGTQLAQLHRCTQKDFGFLSDNFIGSLPQSNRLHPDWAEFYWHERISPQLKLAYDH
                     QLLHKKEIPSLENALPVFREIFGVVKPSLLHGDLWAGNYLISTDGTPYLIDPAAYYGH
                     SLVDIAMSKLFGGFTSSFYDAYHEIIPKSENYSQQIELYQLYYLLVHLNLFGSGYHSG
                     VSAILKRYF"
     gene            3589..>4040
                     /locus_tag="PLI30_10710"
     CDS             3589..>4040
                     /locus_tag="PLI30_10710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI80061.1"
                     /translation="MYILTIGETEREIHWMCFDSLEAGRQFLSLLPGYGCELNEESGN
                     IEREWLRPSEFPDYGEIDFRGNKMPVSRFMFQDQEKVEVYFREIPNLSVEKQGLIDGV
                     TSVDAYSIPNEETRDYISTRENTFKVVKSILAERGIETERAFHGSEDG"
BASE COUNT         1189 a          920 c          958 g          963 t
ORIGIN      
        1 cacgaaaagg atttcattac gaaatccttt ttttgtattt ttgcacttta attcagaagc
       61 gtaatggaaa tctcaagtaa gtacaaccct gcggaggtag aagacaaatg gtattcatac
      121 tggatggaac acaaactgtt tcgttccaag cccgatgaac gtgaacctta tacaattgtc
      181 attccacccc cgaacgtcac cggtatcctg cacatggggc atatgctcaa caacaccatt
      241 caggatatcc tgatacgacg tgcacggatg gagggaaaaa atgcctgttg ggttcccgga
      301 accgatcatg cctccattgc taccgaagcg aaagtggttg ccaagcttgc tgcggaagga
      361 ataaaaaaat ccgaccttac gcgcgaagaa ttcctgtccc atgcctggga gtggacacac
      421 aagcatggcg gaatgattct ggaacagttg aaaaagctgg gtgcatcgtg cgactgggac
      481 agaacctgct ttaccatgga cgaaaaacgt tccgaaagtg taatcaacgt tttcgtggat
      541 ctgtacaaca aagggctcgt nnnnnnnnnn ggtgtccgta tggtaaactg ggatccgcag
      601 gcacttaccg cactctcgga tgaagaggtg gtgcacaagg aggtgaacgg gaaactttat
      661 tacttgcgtt ataaaatcga aggagaagag acctatgcgg ttgtagccac gacccgtccg
      721 gagaccatta tgggcgacac ggcgatgtgt atccatccga ataatcctaa aacggcacat
      781 ttgaaaggga aaaaagttat tgtcccgctc atcggccgcg ttatccccgt tatcgaagac
      841 gaatacgtgg atatggagtt tggaacggga tgtctgaaag ttacgccggc ccacgatgtc
      901 aacgattatg tgctgggaga aaaatacaac ctgccgtcca tcgacatttt taacgataac
      961 ggtacgctca acgaaaacgg agcgatgtat gctggaaaag accggttcga agttcggaaa
     1021 ctcatcgaaa aagacctgga aaatgcgggc ctgattgaga aaaccgaacc ctataccaat
     1081 aaagtcggtt tttctgaacg aaccaacgtc gccattgaac ccaaactctc catgcagtgg
     1141 ttcctaaaaa tggaagacat tgctaaacct gcccttgatg cggttgaaaa cgataccatt
     1201 aaatttcatc cggtgaaata caaaaatacc tatcgccact ggatggaaaa tatcaaggat
     1261 tggtgcatca gcagacaatt gtggtgggga catcagattc cagcatatta tcttcccaaa
     1321 ggcggagtgg tggtagccgt gacaaaagag gaggcttacc aaaaggcgct tcaaacggtg
     1381 gattacccac tcaccataga cgacttgcgt caggacgaag attgcctcga cacctggttt
     1441 tcatcgtggc tatggcccat ctcggttttc gacggaatta accagcccga caatccggat
     1501 atcagttact attatcctac aaacgatttg gttaccgggc ccgacatcat tttcttctgg
     1561 gtggcccgaa tgattatggc gggatatgaa taccgccagt ctctctgttt cagcaacgta
     1621 tacttcacgg gaatcgttcg cgacattcaa ggccgaaaga tgtcgaaaca gcttggaaat
     1681 tcacccgatc ccattgaatt gatggcaaga cacggcgccg acggcgtccg aatgggtatg
     1741 ttgctggcct catcagcggg aaacgatttg ctgttcgatg aatcactctg tgaacagggc
     1801 agaaacttca acaacaaaat ctggaacgct ttccggctgg taaaaggctg ggaagtaaac
     1861 gacacaaatg aacagccaga aacggcaaaa attgccatca actggttcgg gaacgtattg
     1921 tcgaaaacca ttgtcgagct ggatgattta ttcagtaagt acaggctttc ggaggcattg
     1981 atgatggtct ataaactatt ctgggacgag ttttcatcgt ggtacctcga attggtaaaa
     2041 ccggcttacc gacaacccat agaccgggca acctatcagg cgacactagc gttcttcgac
     2101 agcctactgc gcctgttaca cccgtttatg cccttcatca ccgaagaatt gtggcaggcc
     2161 ctgaacgaaa ggaaaccggg tgaaagcatc acgatcgctc aacagccaaa ggctaatcgg
     2221 gtggatgaaa aactgatcgc cgatttcgaa attaccaaac aaattattgc cggcatccgt
     2281 actatccgtt tgcagaagaa tattcccaat aaagatgaat tgacactgca ggtgatcggg
     2341 aatcacgata gccggttcga tacggctgtt gccaaaatgg caaacctctc atcgatcgag
     2401 aacctatcgg agaagcctgc cgtcaccatc ggttttttgg tgggtaccac cgagttcatc
     2461 gtcccgatga gcgataagat ggacgtggaa gccgaattaa aaaagatgaa agccgaacgg
     2521 gagtactacg caggcttctt gcaatcggtg atgaaaaagc tgggtaacga acgtttcgtc
     2581 caaaatgcga aacccgaaat cgtggaaaat gagcgcaaaa aacaagccga tgcggaaagt
     2641 aaaattgcgt tgctcgaaga aagtattaag gcgttggagc aataaccaat aatgcaaaca
     2701 atcctatctc atatttccca ggaaatcgga gaaggcattg cctcataccg ctccattccc
     2761 ggaggcgata tttcttcagc atatcaactg caaaccgaaa ccaaaaagta ttttctcaaa
     2821 gtaaacacca acccgtttgc gttggcaatg ttccatgcgg aacaaaaagg gttgcagacg
     2881 attgaaaaaa caaagactat tgccataccg cacgtgtatg tagtggggtc gcttgaagga
     2941 aaatcatttc tgctgatgga tttcgtggaa gccaaacgtc ctgacgcaaa cgatttcagg
     3001 cagtttggca cgcaactcgc ccaattgcat cggtgtactc aaaaagactt tggttttttg
     3061 tcggataact ttatcggaag cttgccgcaa agcaaccgcc ttcatcccga ctgggcagaa
     3121 ttttactggc acgagcgtat ttccccgcaa ttaaagctgg cttacgacca ccagttactc
     3181 cataaaaagg agattccctc gcttgaaaac gcacttccgg tttttcgtga aatttttgga
     3241 gttgtaaagc cgtcactctt gcacggagac ttgtgggctg gaaattacct catctccacc
     3301 gacggaaccc cttaccttat cgatcctgcc gcctattacg gacactcact ggtagatata
     3361 gccatgagca aacttttcgg cggtttcacc tcatcattct acgacgccta tcacgagatc
     3421 attcccaaat ctgaaaacta ttcgcagcag atagaactct atcaattata ttatctgctt
     3481 gttcatttaa atttgtttgg aagcggatat cactccggtg tttcggctat tttgaaaaga
     3541 tatttctaac tctgcaactg ctgttaattt aatcgcataa gaaaaatcat gtatatactc
     3601 actattggag aaaccgaacg agaaatacat tggatgtgtt tcgattcgtt agaagccggc
     3661 agacagtttt tgtcgctgct tccgggctac ggctgcgaac tgaacgaaga gagcggaaat
     3721 atcgagcgtg aatggcttcg tccgtccgaa tttccggatt acggggaaat cgacttccgg
     3781 ggaaacaaaa tgcccgtcag ccgatttatg tttcaagacc aagagaaagt cgaggtttat
     3841 tttcgcgaaa tccccaatct aagtgttgaa aaacaagggt taattgacgg cgttacttcc
     3901 gtggatgctt actccatacc caacgaagag acgcgagatt atatttcgac aagagagaat
     3961 acatttaaag tcgtgaaaag catattggct gagaggggca ttgaaacgga gcgggctttt
     4021 cacgggtcgg aagatggcga
//
LOCUS       DAOSDR010000296         4029 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrimonas sp. isolate AMR_MDS_1826
            NODE_7868_length_4029_cov_4.088324, whole genome shotgun sequence.
ACCESSION   DAOSDR010000296 DAOSDR010000000
VERSION     DAOSDR010000296.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863355
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrimonas sp. (wastewater metagenome)
  ORGANISM  Petrimonas sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Dysgonomonadaceae; Petrimonas.
REFERENCE   1  (bases 1 to 4029)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4029)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:10:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,720
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 35
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4029
                     /organism="Petrimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7868_length_4029_cov_4.088324"
                     /isolate="AMR_MDS_1826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2023866"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            75..968
                     /locus_tag="PLI30_10715"
     CDS             75..968
                     /locus_tag="PLI30_10715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006801485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HOI80062.1"
                     /translation="MKENNKALLFTVIAVASWSTVASAFKIGLRHYGYFELILVSAVT
                     AMLIFAGVITVQKKWPLLRRLTLKETGSYAFTGLLNPAIYYLILFKSYDLLPAQIAQP
                     INYFWPILLALLLAVIEKKPVPLFKFIGMTISFGGVVLISVGTEGIAGVELSKPGVLL
                     AFFSAFLWASFWIVNRRNKNVDGILGLFLSFMFGSVYLSLATLFVPVSLGSLQGFLSA
                     VYVGLFEMAVPFIFFGLALQKTTNPALTNQLCYLSPFISLFLIHAVLGETIYFTTYMG
                     LFLIVFGILLNEYLSKRRVAV"
     gene            1163..1897
                     /locus_tag="PLI30_10720"
     CDS             1163..1897
                     /locus_tag="PLI30_10720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019541218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NigD-like protein"
                     /protein_id="HOI80063.1"
                     /translation="MKITPFLLTAGMLLGFLLLPSCLDDDNDVNRLYPNALVTVKHAG
                     DKTVFLQLDDKTTLLPVNLTTSPFGEKEVRALVNYEEVDKPGSGYDQAVHVNWIDSIR
                     TKPMAPNLGTENDTKYGIDPVEIVGDWVTVAEDGYLTLRFRTQWGYPRVVHSVNLIPA
                     NNPENPYEVEFRHNSNGDIGERFGDGLVAFRLNSLPDTEGNTVKLKLKWKSFSGEKSV
                     EFDYKTRAATPASSAIAAERSVIPLQ"
     gene            2047..2616
                     /locus_tag="PLI30_10725"
     CDS             2047..2616
                     /locus_tag="PLI30_10725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019541217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HOI80064.1"
                     /translation="MIRDENKEQALLQQIVRGNTMAMKKFYDTYSGYLTAVCSRYIAD
                     KEDVKDILQESFIKIFKAVDKFEYKGAGSLKAWSARIVVNEALRHIRESNKLKVINLP
                     GEELPDMADDREPDFNEIPTQVILEMIRKLPVGYRTVFNLYVFEEKSHKEIASILSIS
                     ESTSASQLHRAKGLLMQEIDLYRFKKMAL"
     gene            2613..3869
                     /locus_tag="PLI30_10730"
     CDS             2613..3869
                     /locus_tag="PLI30_10730"
                     /inference="COORDINATES: protein motif:HMM:NF024895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HOI80065.1"
                     /translation="MNNQWQDNLRNRMEHHEEPVPEGLWESVEQVISAVSSVGTKPAG
                     QRIRLWDKRIGAVAAVGIVLFFIGLYTLKENQKNIQLVEQRQPELPAPVAHPDKEPLL
                     AHRTENEKPETEKKLRGAKGEETAKTAETVRTADVVEAAERQSEEIPRQGEPENSPSP
                     RVDRKYSPETNDGQAFRLPARRRNGRPAKWQTDLYASNIPSGSSKHYDGYRSFTPFGL
                     KSEEEEHVLAAGSGLPGDFIVGKEYRHVYTDVKHFQPVTLGVSLKCNLSDSWSITSGL
                     NYTVLSSQLSTGSYNHYYNSRQTLHYLGLPVTVNYTVWRSGQLSTYISGGGMVEKNVA
                     GALSTDYIVDNKIEERSQEGLSVKPLQWSVSSAAGMEYRLWKGIGLYAEPGVTYHFRN
                     SSGVETIYREKPLNFNIRLGLRFSFF"
     gene            complement(3872..>4029)
                     /locus_tag="PLI30_10735"
     CDS             complement(3872..>4029)
                     /locus_tag="PLI30_10735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005856895.1"
                     /note="uses the energy from reduction of ubiquinone-1 to
                     ubiquinol to move Na(+) ions from the cytoplasm to the
                     periplasm; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit F"
                     /protein_id="HOI80066.1"
                     /translation="QVIHDTYLKDHDAPEDIEYYMCGPGPMAAAVQRMLDSLGVPPEM
                     IMFDDFG"
BASE COUNT         1008 a          940 c         1093 g          988 t
ORIGIN      
        1 cgccgcacag gaagtgtcgg aacagaaaga aattatcact atttttgcgt gccgcattat
       61 tcgggttgct tcgaatgaaa gaaaacaata aagcactttt gtttactgtt attgctgttg
      121 ccagctggtc gacagttgct tctgctttca agatcgggct gcgtcactac ggctactttg
      181 agctgatcct ggtttcggcc gtcacggcga tgctgatctt tgcgggggtt atcacggtcc
      241 agaaaaaatg gcctctgttg cggaggctga cccttaagga gacggggagc tatgctttta
      301 ccggcttgtt gaatcctgcc atttattacc tgatcctttt caagtcgtac gatttgttgc
      361 cggcgcagat tgcgcagccc atcaattatt tctggcccat cctgttggcc ctgttgttag
      421 cggtgatcga gaaaaagcct gttcccttgt tcaagtttat cgggatgaca atctcgtttg
      481 gcggcgtggt gctgatctcg gtgggaaccg aagggatcgc cggagtagag ctctcaaaac
      541 cgggagtctt actggctttc ttcagtgcgt tcctgtgggc ttccttctgg atagtgaacc
      601 gacggaacaa aaacgtggac ggtatcctgg gtttgttcct gagttttatg ttcggttccg
      661 tctatctgtc gctggccaca ctctttgtcc cggtcagcct gggttccttg caggggtttt
      721 tgtcggccgt ttacgtgggg ttgtttgaaa tggcggtgcc gtttatcttt ttcgggctgg
      781 cgttacagaa gaccaccaat cccgcactta ccaatcagtt gtgttacctt tctcccttca
      841 tttccctttt tcttatccat gcggttttgg gtgaaaccat ctattttacc acctacatgg
      901 gcttgttcct gatcgttttc ggcattctgc taaacgaata tttaagcaag cgcagggttg
      961 ccgtttaagc tgaaaatatc aaccgttgtg aggcaagctc gcttggatcc atgctggacg
     1021 gtggcgaaat ctgtgaggcg aattctttaa ggctattagg attaagaaaa aataacattt
     1081 tctcgtgcaa ggtttcggct tttgccggat ttatagggta actgtacaaa cacgaatgca
     1141 aaaattaaaa acaatgaaaa ctatgaagat tactcctttt cttttaacag cgggaatgct
     1201 gttgggcttt ctgcttttac cgtcctgcct ggacgacgac aatgatgtga accgcttata
     1261 tccgaatgcg ctggtgaccg ttaagcatgc gggggataaa actgtttttc tgcagttgga
     1321 cgacaagacg acactgcttc ccgtgaacct gacaacctca ccctttgggg aaaaagaagt
     1381 gcgggcgctg gtaaactacg aagaagtgga caagccaggc agcggatacg accaggcggt
     1441 acacgtgaac tggatagaca gtatccgtac gaaaccgatg gcgcctaatc tggggactga
     1501 aaacgatacg aagtacggta tcgaccccgt tgagattgtg ggcgactggg tgaccgttgc
     1561 cgaagacggc tatctcacgc tgcgattcag gacgcagtgg ggctatccgc gggttgtcca
     1621 ctccgtgaac cttatcccgg ccaacaatcc cgaaaacccg tatgaggtgg aattccgtca
     1681 caactccaac ggagatatag gagaacggtt tggcgacggc ctcgtggctt tccggctcaa
     1741 cagcctgccc gacacggaag ggaatacggt aaagctgaag ttgaaatgga aatcgtttag
     1801 cggcgagaaa tcggttgagt tcgactataa aacgcgtgcc gcgactcccg ccagctctgc
     1861 catcgctgct gagcgcagcg tgatcccatt gcaataaccg cttgccggga gcatccgctt
     1921 cggcaaccgt gccacaaagg gttgtttcaa aaatcgacaa aaggctgtac ctttgcgtga
     1981 taaaggtaca cctttttcgt aaagggagag cgtgtatttt agtgttggtt cgaacaaaat
     2041 ccgttaatga tcagagacga aaacaaagag caggcccttc ttcagcagat tgtacgcggt
     2101 aatacgatgg ccatgaagaa gttttacgac acctattcgg ggtatcttac cgcggtatgc
     2161 tctcgttaca tcgctgacaa agaggatgta aaagatatct tacaagaaag cttcatcaaa
     2221 attttcaaag cagtagataa gttcgagtat aaaggggcgg gatcgttaaa agcgtggtcg
     2281 gcccgcatcg tagtcaatga agcgttgaga catatccggg aaagcaataa gctgaaggtt
     2341 ataaacctcc ccggtgagga gctgcccgac atggccgatg accgtgagcc tgatttcaat
     2401 gagataccca cgcaggttat cctggaaatg atccggaagt tacctgtggg ctaccggacc
     2461 gtatttaacc tgtatgtttt tgaggaaaaa agtcacaagg aaattgcttc gatactgagc
     2521 atttctgaaa gtacttctgc ctcgcagtta cacagagcca aaggcttgtt gatgcaagag
     2581 atagatttgt atcggtttaa aaaaatggca ctatgaataa tcaatggcag gataacctgc
     2641 gcaatcggat ggagcatcac gaagagcctg ttcccgaagg tttgtgggag agcgtcgagc
     2701 aggtgattag cgccgtgagc tccgtaggga caaagccggc aggacaacgg atccggttgt
     2761 gggacaagcg tatcggggca gtagcagcag tggggattgt tttgtttttt atcgggttgt
     2821 acacgttgaa ggaaaatcaa aaaaacatac agctcgtaga acagagacag ccagaactgc
     2881 ctgctcccgt tgcgcatccc gacaaagaac cgctcttggc gcaccgtact gaaaatgaaa
     2941 aaccggaaac ggaaaaaaag ttgcgtggag ctaaaggaga ggaaacagct aaaacagcgg
     3001 aaacagtgag gacagcggat gttgttgaag cggctgaacg gcaaagcgaa gaaatcccgc
     3061 gtcagggaga gcccgaaaat tctccttctc cccgtgttga ccggaaatac agtccggaaa
     3121 ctaacgacgg gcaggcgttt cgcttgccgg cacggcggcg taacggcagg cccgcaaaat
     3181 ggcagacgga cctttatgcg tcgaacatcc cgtcgggttc ctccaaacat tacgacggat
     3241 accgcagctt tacccctttt gggttaaaat cggaggaaga ggagcatgtg ctggccgcag
     3301 gttcgggttt gcccggcgat tttattgtgg ggaaggagta ccggcacgtc tataccgacg
     3361 tcaagcattt tcagccggtc acgctgggtg tttcgctgaa gtgcaacctc agcgacagct
     3421 ggagcatcac gagcggcctc aactataccg ttctctcctc gcagctaagc acgggaagct
     3481 acaatcatta ctacaacagc cggcagacgc tgcactacct gggcctcccg gtaactgtta
     3541 actacaccgt ctggcgctcc ggccaattat caacgtatat ctccggcggt gggatggtgg
     3601 agaagaacgt cgcgggcgca ctgtccaccg attatattgt ggacaataaa attgaagagc
     3661 gtagtcagga agggttgtcc gttaaaccgc tgcaatggtc tgtaagctcc gccgccggca
     3721 tggagtaccg gttgtggaaa ggcatcggat tgtatgccga gccgggagtg acctaccatt
     3781 tcaggaacag tagcggagtg gaaacgattt atcgggaaaa gccgttgaac ttcaacatca
     3841 ggctgggact gcgcttctcc tttttttagg tttatccgaa atcgtcgaac atgatcattt
     3901 ccggcggaac gccgagactg tcgagcatcc gctgtacggc tgccgccatt ggtccggggc
     3961 cgcacatgta gtattcaata tcttcaggag catcgtgatc cttcaagtac gtgtcgtgaa
     4021 tcacctgat
//
LOCUS       DAOSDR010000297         4020 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrimonas sp. isolate AMR_MDS_1826
            NODE_7889_length_4020_cov_4.438588, whole genome shotgun sequence.
ACCESSION   DAOSDR010000297 DAOSDR010000000
VERSION     DAOSDR010000297.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863355
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrimonas sp. (wastewater metagenome)
  ORGANISM  Petrimonas sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Dysgonomonadaceae; Petrimonas.
REFERENCE   1  (bases 1 to 4020)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4020)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:10:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,720
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 35
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4020
                     /organism="Petrimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7889_length_4020_cov_4.438588"
                     /isolate="AMR_MDS_1826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2023866"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            483..1571
                     /gene="pdhA"
                     /locus_tag="PLI30_10740"
     CDS             483..1571
                     /gene="pdhA"
                     /locus_tag="PLI30_10740"
                     /inference="COORDINATES: protein motif:HMM:TIGR03181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045250 - cytosolic pyruvate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0004739 - pyruvate dehydrogenase
                     (acetyl-transferring) activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring) E1
                     component subunit alpha"
                     /protein_id="HOI80067.1"
                     /translation="MIFEEFNPSEDKILRIIDNDGRILRPDLFPEISDDTIVKAWKAM
                     LFERVADEMAVSFQRQGRMFTYPPNKGQEAIHIAAGNVIRENDWLVPAFREVGLMLAK
                     GVTMKEIFLYYNGNEQGSNFKNAPRVLPINVPIGTQLPHAVGIGYSIKYRKEGDIVFT
                     YIGDGGTSEGDFSEALNFAGVWQVPVVFTIQNNQYAISVPVTTQTKSINLAVKSVAFG
                     IPGIKVDGNDFFAMYLVYQKASEHARTGKGPVLIEALTYRCGAHTTSDDPSRYRTKEE
                     EAAWELSDPLFRLKKFMEHKGIFRENEEKLREQYKKEVEKQFLDAENVAPYALEDVFN
                     YMYVEMPDDLKKQKEEYQQFLAWKGNRK"
     gene            1568..2545
                     /locus_tag="PLI30_10745"
     CDS             1568..2545
                     /locus_tag="PLI30_10745"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019541064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HOI80068.1"
                     /translation="MSVMTMVQAINNALDIKLAEDNGVVVYGEDVGVVGGVFRVTEGL
                     QKKYGVERVFDSPLAESAIVGTGVGMALSGLRPVVEMQFEGFSYPAFNQIISNVSRMH
                     NRSRGRFRIPMVIRFPYGGGINAIEHHSESPEALYSHIPGLKVVVPSTPHDAKGLLIS
                     AIESDDPVIFMEPKRIYRAIKQEVVTGKFTIPIGKASVLTEGTDVTVVAFGAMIRECQ
                     RAMVMAKEAGISVELIDLRTIYPIDKETIRNSLQKTGRLVVVAEAQRSFSVGSELIAI
                     ANEEAFLYLEAPPKRVMGFDTIIPLAQGEKYFMITPERIFYEIEKTVKF"
     gene            2652..3926
                     /locus_tag="PLI30_10750"
     CDS             2652..3926
                     /locus_tag="PLI30_10750"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012424.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase family
                     protein"
                     /protein_id="HOI80069.1"
                     /translation="MRYIFNFPDIGEGLEEGTILEWYVTKGQAVSTGDSLVKMETDKV
                     VADIPSPRTGIVAARFGEVGDVIKVGSPLVEIEIEGVFAEEAQAEAKRGFDPEPEEKA
                     ETVVGTMEVASRNAFLPASNEGTTEQPPVTEKPRRKALSTPVARAMARDLGIDINEVC
                     GTGPSGRVTKADVENHKSVRKPSSLRVSEQEDDEVTYEPLTQIRKAIARNMIQSKHNA
                     AHMSLFEEVEISDLVRVREKYKGKFAERGVKLTYLAFIVKAVVNALKQHRQLNSQIDL
                     ENDRMIFNNRYNIALAVDTPAGLVVPVVRDADRLSIFEIAQRIDEISEKARKRDLTLE
                     DMKGGTFTITNYGSIGGIFATPVINYPQAAILGVGRILKTPVVKDDAIVVGRVMALSL
                     SVDHRIVDGGEVTRFINKVMEYLADPMALLME"
BASE COUNT         1140 a          830 c         1050 g         1000 t
ORIGIN      
        1 atggatttta tcggttttca acttgcggat aatgtctttc agcacagtta aggtaacttc
       61 cccgccttcc ggtgcccgtg tatcggccac ctcttctttt atcaggtttt gcccttctat
      121 taaaccgcct ttgatggtgg ttccacccaa atctattccg atgatctgtt tcattgtgtt
      181 gtattttaat ggttgagatg tacaaaacta agcaatattg gtgaattaaa cgaatgttat
      241 ggacaaagtc tctggcttaa gcggcttaac aggtcgtcaa gccttaactt gagactgaag
      301 aagtgaacaa aattccgaaa ataacgataa acagtgccgg aaaatgaata aatttacggt
      361 ttgggcaaac cttaaaccaa gttattcgtt atagtctgta gtttttacat tttaaaatca
      421 ttccaaataa ttcactttcg tatgttttct gacgagatga atgcagctaa agataagacc
      481 ttatgatttt tgaagagttc aatccatcgg aagataagat cctcagaatt atcgataacg
      541 atggtcgtat tttacgtccc gacctattcc cggaaatatc cgacgatact attgtgaaag
      601 cctggaaagc catgctcttt gaacgggtag ccgatgagat ggctgtatcc tttcaacgcc
      661 agggacgcat gttcacttat ccccccaaca aaggacaaga agctattcat attgcggccg
      721 gcaacgtgat acgcgaaaac gactggctcg ttcctgcttt tagggaagtg gggctaatgt
      781 tggctaaagg cgttacaatg aaagagattt tcctgtatta caacggcaac gaacagggga
      841 gtaactttaa aaatgccccg cgtgtattac ccataaacgt tcctatcggt acacagttgc
      901 cccatgccgt tgggatagga tattccatta aatacaggaa ggaaggcgat attgtgttta
      961 cttatattgg ggatggcggt acgtcggaag gtgacttcag cgaagcgttg aactttgccg
     1021 gtgtgtggca ggtcccggta gtgtttacca tacaaaacaa ccagtacgct atttccgttc
     1081 cggtaaccac gcaaaccaaa tctataaatt tagctgttaa atcggtagcg tttggcattc
     1141 cgggcatcaa agtcgacgga aatgatttct tcgccatgta cctggtctac caaaaggcat
     1201 cggaacatgc gcgaaccggt aaagggccgg tgcttattga ggcacttacg tacaggtgtg
     1261 gcgcccatac cacttccgac gatcccagcc gctatcggac aaaagaggag gaggcagcgt
     1321 gggagctgtc ggatccgttg ttccggttga aaaaatttat ggaacacaaa gggatattta
     1381 gggagaacga agaaaagctg agggagcagt ataaaaaaga agtggagaaa caatttctgg
     1441 atgctgaaaa cgttgccccg tatgctttgg aggatgtttt caattacatg tatgtggaaa
     1501 tgcctgacga cctgaaaaaa cagaaagaag aatatcaaca atttttagct tggaaaggga
     1561 atcgaaaatg agcgtaatga caatggttca ggcgatcaac aacgcgttgg atattaagct
     1621 tgccgaggat aacggtgttg tggtttatgg ggaagatgta ggagttgtgg gaggagtatt
     1681 ccgggtaacc gaggggttgc agaaaaaata cggtgtggag cgtgtgttcg attcgcctct
     1741 ggcggagtcg gctatcgtgg gaacaggagt gggtatggcc ctttccggct tacggcccgt
     1801 ggtggaaatg caattcgaag ggttttccta tcctgcattt aatcagataa tctccaatgt
     1861 atcgcgcatg cacaaccgtt cacgcggaag gttcaggatt cccatggtca tccggtttcc
     1921 ctacggtgga ggcatcaatg ccatcgaaca ccattcggaa agccccgaag cactttacag
     1981 ccatattccc ggtttaaaag tagtggttcc ttcaacgccg cacgatgcaa aagggttgct
     2041 gatatctgct attgaaagtg acgatccggt tatttttatg gagcccaaac gaatctatag
     2101 ggccatcaag caggaggtgg ttaccggtaa atttaccatt ccaattggaa aagccagcgt
     2161 gcttaccgag ggtacagatg ttaccgtcgt ggctttcgga gcgatgattc gcgaatgcca
     2221 gcgggcaatg gtaatggcga aggaggcagg tatatcggtg gagttaatcg atctccggac
     2281 gatctatccc atagacaagg agaccatccg gaactcgttg cagaaaacag gccggttagt
     2341 ggttgtggca gaagcacaac gatcattcag cgtgggttcg gagttgatag ccatagccaa
     2401 cgaagaggct ttcctctacc tggaagctcc tccaaaaagg gtgatgggtt ttgataccat
     2461 tattccgttg gcgcaaggtg agaaatattt tatgataact cccgaaagga ttttttatga
     2521 aatagaaaaa acggttaagt tttaaaaaca gcatgaatga agtaactgtg cgaagcgagt
     2581 aacacgaagt gaatttcgcc gtaggccaat tacaattaat gttcaaacca taaatgatta
     2641 aacgaactta aatgaggtat atttttaatt ttcccgatat aggagaggga ctcgaggaag
     2701 gaaccatcct ggagtggtac gttacgaaag gacaggcggt aagtacgggc gattccttgg
     2761 tgaagatgga aaccgacaag gtagtggccg atattccgtc accgagaacg ggaatagttg
     2821 ctgcccggtt tggagaagta ggtgatgtca taaaagtagg cagcccgcta gttgaaattg
     2881 aaatagaagg agtttttgcg gaagaggctc aggccgaggc gaaaagagga tttgaccctg
     2941 aaccggagga gaaagccgaa acggtggtgg gtacgatgga ggtggcttcg cgaaatgctt
     3001 ttttgcctgc gagcaacgaa ggcacaacgg agcagcctcc cgtaacggaa aaaccacgcc
     3061 gtaaagcttt gtctacgccc gttgcgcggg ctatggccag ggatttaggc atcgatataa
     3121 atgaagtctg tggcacaggg ccttcggggc gggtcacaaa agcagatgtt gaaaaccata
     3181 agtccgtcag aaagccatcc tccctccggg tttcggaaca agaggacgat gaggtaactt
     3241 atgagcccct tacccagatc cgcaaggcta ttgcccggaa catgatccag tccaagcaca
     3301 acgctgccca catgtcgttg ttcgaagaag tggagatatc cgatctggta agggttcggg
     3361 agaagtataa ggggaaattt gccgaaagag gggttaagct gacctacctc gcttttattg
     3421 taaaggctgt cgtcaatgcc ctcaagcaac acaggcagtt aaattcgcag atcgacctgg
     3481 agaatgaccg gatgatcttc aacaaccgct ataacatagc gttggccgtg gatacccctg
     3541 ccggactggt ggtccctgtt gttagggatg ccgaccgttt aagcattttt gaaattgctc
     3601 aacgcatcga cgaaatatcg gagaaagcca gaaaaagaga ccttaccctg gaagacatga
     3661 aaggcggaac atttaccatt accaactacg gttccatcgg aggaatattt gccacgccgg
     3721 tgattaatta tccccaggcg gctattttgg gagtgggcag gatcctcaaa acccctgtgg
     3781 tgaaagacga tgccattgtt gtaggccgtg taatggcatt gtcgctctcg gtagatcacc
     3841 gtatcgttga tgggggagaa gttacccggt ttatcaacaa ggtaatggaa tatcttgccg
     3901 atcccatggc gttactgatg gaataacaca ccttatttga aacacaaaac aactcatagt
     3961 atggattacg acgtattgat catcggtgcc ggaccatccg gttatgtagc cgccatccgg
//
LOCUS       DAOSDR010000298         4015 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrimonas sp. isolate AMR_MDS_1826
            NODE_7901_length_4015_cov_4.027525, whole genome shotgun sequence.
ACCESSION   DAOSDR010000298 DAOSDR010000000
VERSION     DAOSDR010000298.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863355
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrimonas sp. (wastewater metagenome)
  ORGANISM  Petrimonas sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Dysgonomonadaceae; Petrimonas.
REFERENCE   1  (bases 1 to 4015)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4015)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:10:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,720
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 35
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4015
                     /organism="Petrimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7901_length_4015_cov_4.027525"
                     /isolate="AMR_MDS_1826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2023866"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(30..539)
                     /locus_tag="PLI30_10755"
     CDS             complement(30..539)
                     /locus_tag="PLI30_10755"
                     /EC_number="2.7.1.156"
                     /EC_number="2.7.7.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019538980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0008820 - cobinamide phosphate
                     guanylyltransferase activity [Evidence IEA];
                     GO_function: GO:0043752 - adenosylcobinamide kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional adenosylcobinamide
                     kinase/adenosylcobinamide-phosphate guanylyltransferase"
                     /protein_id="HOI80070.1"
                     /translation="MRKIIFITGGQRSGKSSFAQRLAEKYATDPLYLATARHWDEDFE
                     KRIQRHQADRGETWTTVEEEKLLSRHDFNGKTVLVDCVTLWLTNIFHDNGFNPETSLE
                     EAKAEWDRFINQDFTLIAVSNEIGMSLHAPGETARHFTDLQGWVNQHIARTADEVFFM
                     VSGIPVQIK"
     gene            complement(527..916)
                     /locus_tag="PLI30_10760"
     CDS             complement(527..916)
                     /locus_tag="PLI30_10760"
                     /inference="COORDINATES: protein motif:HMM:NF025833.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4430 domain-containing protein"
                     /protein_id="HOI80071.1"
                     /translation="MKLKIISLLTTSLLVLTLQAQEKVTVTINYGEEKLSQAFQVNWQ
                     EGMTAMLAVQSCANVTTHPVKDYIFVSTINGISTVAGKKAWYYTVNNESTHKLAFRYP
                     VNPGDTVEWIYKKDVCSAIKQKQPCEK"
     gene            complement(928..3060)
                     /locus_tag="PLI30_10765"
     CDS             complement(928..3060)
                     /locus_tag="PLI30_10765"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOI80072.1"
                     /translation="MYKKILSGVAALLAFAGMLQAQETVKDYDLDEVVVTATRMNLPL
                     KSIPQKVEIIDKDKILSIPAENAAELLKRATNLDMIQYPGASATVGMRGFSPTAHARS
                     YTLVMIDGLPSGTSNLTTLPTSVIERIEVIKGPYSVLYGTDAMGGVINIITKKATLQK
                     TGEVSLKGGSFGTTSFDANAAGMLSDRVGVVVGFSNQIQQKDYRIGGKNLLKISKTEK
                     LILDEKSYDDVYPNTTFQMSQLFGKLNYSVNNQWNVSVSSFYNLASDVETPGNYFTQI
                     ASKKDIGRLNLFSEISHTSENNKLIIAPYYSNEKNWNYETAASDKTNFISFKDDVSEY
                     GVRTNNTYYLNHITLLAGMDYDVLDYQSTRQSKKGNEAGPYKPNNRNQRLSAFVQTNY
                     ELGDLSINGGLRANYIRYKIFKHDSLSNKSTSDNYFYLIPSLGLKYNLPKGFNVHASV
                     GKAFSIPDAFATSGFYEVNTLVGEWPFRMAYVGNADLKPETSITYEGGMGYYRDTFYF
                     DLTYFNTTHKNKIVTRRTSGMGNEADSTHYENAKSASMSGLEFIGSINIGQLVGWNNK
                     VEIYGGLTYMFNNTFTEFATEKVKDLLFVNKANGNFGISYDNRKGFTTRLHARYKGRR
                     LERDGFSRARPLITKENYYTKGGYKETDKILQFSDHLIFDYSAFYNLTPKARIGISVS
                     NLFDENYTEKDGYNMPGRNIMGHASYFF"
     regulatory      complement(3198..3401)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            complement(3457..>4015)
                     /locus_tag="PLI30_10770"
     CDS             complement(3457..>4015)
                     /locus_tag="PLI30_10770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019538995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOI80073.1"
                     /translation="QFTETTHITVEEYVLMGRMPYRKPFQFFDKAEDIDIAHHYMQLT
                     NTYRLRDKSMAELSGGEQQMASIASALCQNPALLLLDEPTSHLDITHQVKFMNLMQKL
                     NDELKLSVIMIVHDLTLAAEYCDYLIMMKNGRIHRKGSPENVLTYENIEHVYDTVVVV
                     KINPVSGKPVVFPVSERRLRELNIP"
BASE COUNT          972 a          847 c          883 g         1313 t
ORIGIN      
        1 ttcttcctgt atcataattg tttgatttat cattttattt gtacggggat acccgaaacc
       61 ataaaaaaca cttcatccgc agtcctggct atgtgttggt ttacccaacc ctgcagatcg
      121 gtaaagtggc gggcggtttc accgggagcg tgcaggctca tgcctatctc gttgcttacc
      181 gcgataagcg tgaaatcctg gttgatgaat ctatcccatt cggcttttgc ttcttccaat
      241 gaggtctccg ggttgaaccc gttatcgtga aagatgttgg tgagccacag cgtcacgcaa
      301 tccaccagca cggttttacc gttgaaatcg tgacggctca acagtttttc ttcttcgacg
      361 gtggtccagg tttcgcctct gtcggcctgg tgcctctgga tgcgtttttc gaagtcctca
      421 tcccagtgcc gggcggtagc cagatatagc gggtcggtag cgtatttttc tgccaatcgt
      481 tgtgcgaatg aacttttacc cgaacgttgt ccgcctgtta tgaaaattat ttttcgcatg
      541 gttgtttttg ctttatggcg gagcagacat cttttttgta tatccattcc accgtatcgc
      601 ctggatttac agggtatcga aaagcgagtt tatgtgtact ttcgttattc acggtatagt
      661 accacgcttt tttaccggca acggtactta ttccgttgat ggtagataca aaaatataat
      721 cttttaccgg atgtgttgtt acattggcgc aactttgtac ggccagcatg gccgtcattc
      781 cttcctgcca atttacctga aaggcttggg aaagcttttc ttctccgtaa tttatggtta
      841 cggtaacttt ttcctgggct tgtagagtta agacaagcag gcttgttgtc agtaaagaaa
      901 taatttttag tttcatgatt tttttattta aaagaaataa gatgcatgac ccataatgtt
      961 ccgtcccggc atgttgtatc cgtctttctc ggtgtagttt tcatcgaaaa ggttggaaac
     1021 ggagatgccg atacgggctt tgggggtaag attgtagaaa gccgaataat cgaaaataag
     1081 atgatctgaa aattgtaata tcttgtcggt ttctttgtaa cccccttttg tatagtagtt
     1141 ttctttcgta atcagaggtc ttgctctcga aaaaccgtct ctttccaacc gacgcccttt
     1201 atatcttgca tgcaggcggg tggtaaaacc tttgcggttg tcgtaggaga ttccgaaatt
     1261 accgttggct ttattcacaa acaagaggtc tttcactttc tcggttgcga attctgtaaa
     1321 tgtattgttg aacatgtagg ttaatcctcc gtatatttcc actttgttgt tccacccaac
     1381 cagttgtcct atatttatcg aacctataaa ctccaatccg ctcattgatg ccgatttcgc
     1441 attttcataa tgagtggaat ctgcttcatt gcccataccg gatgttcttc gtgttacaat
     1501 tttatttttg tgggttgtgt tgaaataggt taggtcaaaa taaaatgtat cccgataata
     1561 ccccattcct ccttcgtagg tgattgacgt ttcgggtttc aagtctgcat ttcccacata
     1621 ggccatcctg aatggccact cgccaaccaa ggtatttacc tcataaaagc ccgatgtggc
     1681 aaatgcgtcg ggaatactga atgcttttcc cacacttgca tggacgttga accctttagg
     1741 caggttgtat tttagtccca gagatggaat cagataaaaa taattgtccg aggtgctctt
     1801 attactcagg ctatcatgtt tgaagatttt ataccggata tagtttgctc ttaatcctcc
     1861 atttatggat aagtctccca actcgtaatt tgtttgaaca aaagccgata accgttgatt
     1921 tctgttgttt ggcttatagg ggcctgcttc atttcctttt tttgattggc gggtagattg
     1981 ataatccagc acatcatagt ccatgcctgc caacagggtt atatgattga gataatacgt
     2041 attgtttgtt ctcacaccat actcactcac atcatcctta aaagagatga aattggtttt
     2101 gtcgcttgcc gctgtttcgt aattccagtt tttttcgttg gaatagtaag gtgcaatgat
     2161 taatttgttg ttttcagacg tgtgtgatat ttcgctgaac agattgagtc tgccgatatc
     2221 ttttttggat gcaatctgag tgaagtaatt tccgggtgtt tccacatccg aagccaggtt
     2281 gtagaacgac gatacagaca cgttccattg gttgttcacg gaatagttta gttttccaaa
     2341 gagttgagac atctgaaacg tggtgttcgg atacacatcg tcatatgatt tttcgtcgag
     2401 gatgagcttc tccgttttgg atattttcaa caaatttttt ccgcctatgc ggtaatcttt
     2461 ttgctgtatc tggttcgaaa aacccacgac gacaccaact ctgtcagaaa gcatgcccgc
     2521 cgcattggca tcgaacgaag ttgtgccaaa acttccgcct ttgagcgaaa cttccccggt
     2581 tttttgtaac gttgcttttt ttgtgatgat gtttattacg cctcccatgg catcggttcc
     2641 gtagagaacc gaataggggc ctttgatcac ttcgatacgt tcaataaccg atgtgggtaa
     2701 ggttgtcaaa ttgcttgtcc cggatggtaa tccgtcaatc attacaaggg tatagctgcg
     2761 tgcgtgcgct gtaggtgaaa atcccctcat acccacggtg gctgacgcac cgggatattg
     2821 aatcatgtca agatttgttg ctctctttag aagctcagct gcattttcgg caggaataga
     2881 caggattttg tctttgtcaa taatttccac tttttgtgga atacttttaa gtggcaagtt
     2941 cattcgtgtt gccgtaacaa caacctcgtc gagatcgtaa tccttaacgg tctcctgggc
     3001 ttgtagcatc cctgcaaaag caagcagggc cgcaactccc gataaaattt ttttgtacat
     3061 aaataaaaaa atttacagtt aaacaattaa tgatttgtgt aaccataaac tttcgagaag
     3121 gaaaagggaa tggcaggcat gccataacct gattgggtct tgtagaaaac accttgcttt
     3181 tatccccgaa agcgtatgat agatgatggc tttcggacag gtcttccggc tcgtttctgt
     3241 tttctgatgc cttcccatcc cggacaacgg aacagtgaca atacaactca gaaaacattt
     3301 tgaaacatac ggctgcgggt acagctttgg attcttccgc ttgaggcggg attaccaaat
     3361 tccatgatgt ctaaaagcgg gtacaaaggt aatgttttat tttaatttca gaaagaaaac
     3421 cctttttatt aaatagagaa cctggtggtt ctggccttat ggtatgttta gctctcttaa
     3481 tcttcgctcc gagacaggaa agaccaccgg ttttcccgat acgggattta tttttacaac
     3541 cacaacggta tcgtaaacat gctcgatgtt ctcgtatgta agaacgtttt cgggactccc
     3601 tttcctgtgg attctaccgt ttttcatcat gatgaggtaa tcgcagtatt cggcagccaa
     3661 tgttaagtca tgcacaatca tgattacgga taatttcagc tcatcgttaa gcttttgcat
     3721 caggttcatg aacttcacct ggtgggtgat gtcgaggtga gacgtgggtt catccagcag
     3781 taaaagggcc gggttctggc agagtgccga agcaatgctt gccatttgtt gttctcctcc
     3841 gctcagttca gccatggatt tgtcgcgaag ccggtacgta ttggtcaact gcatgtagtg
     3901 atgtgcaata tctatatctt cggccttgtc gaaaaactga aacggtttcc tgtagggcat
     3961 tcttcccatc aatacgtatt cttcgaccgt gatgtgggtt gtttccgtaa attgc
//
LOCUS       DAOSDR010000299         4006 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrimonas sp. isolate AMR_MDS_1826
            NODE_7920_length_4006_cov_2.636801, whole genome shotgun sequence.
ACCESSION   DAOSDR010000299 DAOSDR010000000
VERSION     DAOSDR010000299.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863355
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrimonas sp. (wastewater metagenome)
  ORGANISM  Petrimonas sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Dysgonomonadaceae; Petrimonas.
REFERENCE   1  (bases 1 to 4006)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4006)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:10:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,720
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 35
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4006
                     /organism="Petrimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7920_length_4006_cov_2.636801"
                     /isolate="AMR_MDS_1826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2023866"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..962)
                     /locus_tag="PLI30_10775"
     CDS             complement(<1..962)
                     /locus_tag="PLI30_10775"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOI80074.1"
                     /translation="MKRRIFLKNVAVTGVSLTILPSYAVSGLGHVAPSDKLNIAGIGI
                     GGKGKVNLRNMVGQNIVSLCDCDYNYARDVFKSYPKASRYKDFRLMLEKQKDIDAVVI
                     ATPDHTHAVTAAAAMELGKHVYLQKPLTHSVWESRYLSQLAKKTGVITQMGNEGHSSD
                     TVYEVTEVVQSGCLGTVREVHVWTNRPIWRQGMPKPLIKVPVPDLLDWDLFIGPAPMR
                     EYNPEYHPWIWRGWWDFGTGALGDMGCHLLDVPYYALKLGQPVAFQAASSLVNTQSAP
                     ISAKVSYKFPARDNLPYCQLPELELTWYDGGLMPSRPYNLPEDAP"
     gene            complement(1057..2685)
                     /locus_tag="PLI30_10780"
     CDS             complement(1057..2685)
                     /locus_tag="PLI30_10780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009122761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tat pathway signal sequence"
                     /protein_id="HOI80075.1"
                     /translation="MDRREFINKTGWTFVGLAATGSLLQGCPPKSKEAKKTLPSSHNL
                     KLYWGDIHNHCNLTYGHGDMRDAFEAAREQLDFVSVTPHAMWEDIPGRNDMRLQWVID
                     YHTKAFDALRAGKWDEYVKMTSGYNKENEFLTFLSYECHDMAHGDHVALLYDVNGTMA
                     EPSNAMELKARLKGQKAFVTPHHMGYQTGFRGYNWKSFQEDGQTPFVEMYSRHGLAES
                     DDGDYNYLHDMGPRQWEGTVLYGLEQGHKFGIIGSTDQHAGYPGSYGDGRVMALAPSL
                     KRDDIWNALQNRNVGCATGDKIKIDFRINDAIMGEVIRGNSRRIYLNVEGENSVDYVD
                     IIKNGKCIARMNGPLIPTVPKEDPVRCKIKVEFGWNREEEYIHWNGRLSIDKGIINRI
                     TPCFRGAAYTSPQPGEKAFKTRVNRVLSSSEKETILDVYSTKNPNTMTPTVQGVILDV
                     TMPKAGNLTSDFNGKKITHSIGELLQGSKSHFMIGWLSEAILFGRAIPENAFQVEHYM
                     EDTVAEREIDYYYVRVRQKNQQWAWSSPIWVEKR"
     gene            complement(2696..>4006)
                     /locus_tag="PLI30_10785"
     CDS             complement(2696..>4006)
                     /locus_tag="PLI30_10785"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOI80076.1"
                     /translation="KKRVIGANDKVRVGFIGVGNRGTQLLHLFMEEPDCEVAALCDVY
                     EPYVTRDYSAVLDRYKKDMGTRIPKMGENFPSNVKQYKDYREMLGDKSIDAVCIATPD
                     HWHALQTIDAINAGKDVFVEKPLSKTIAEGRRMVEVGNSSRQIVTVGLNRRGAPTFQR
                     LAKEIPAGKIGKVTFASACHVSNMYPSGIGKLQPETPPRNFDWDMWLGPRAYRPYQYN
                     IAPYMFRWWEDYANQISNNGVHYLDLLRWLIDEEAPTAISAHGGKFVLNDDRTIPDTM
                     HVTYEFKSGVLVTLTILEASTGTFIPHGFLELRGTKGTIHTGENDYKITPASAGQFQT
                     WKPMIEPEDYSLAKDDTLLIDGSYKNSASSLVRNFLDCVKSREQPWATLEIGHRSTTM
                     AHLATIAMMTRERLEWDAVNERFTNSEVANKYLSYEYRKPWKLD"
BASE COUNT         1028 a          963 c          759 g         1256 t
ORIGIN      
        1 ggagcatctt cagggagatt ataggggcgt gagggcatca aacctccgtc ataccacgtc
       61 aattccagtt cgggcagttg acagtagggt aaattatctc tggcgggaaa tttgtaacta
      121 acttttgccg atataggcgc actttgtgta ttaacaagag atgatgctgc ttggaatgca
      181 acaggttgtc cgagttttaa tgcataataa ggtacatcca gcaaatgaca tcccatatcg
      241 ccaagagctc ctgtaccaaa atcccaccac cctctccaaa tccatggatg atattcagga
      301 ttgtattctc gcatgggtgc aggacctatg aacaagtccc agtctagtaa atccggaaca
      361 ggtactttta ttaaaggctt tggcatacct tgtctccata tgggcctgtt tgtccacaca
      421 tgcacctcac gtacggtacc taaacaacca ctttgtacta cttctgtcac ctcatacacg
      481 gtgtcagaag aatggccctc atttcccatt tgagttatta ctcctgtttt ttttgctaac
      541 tgagagagat atcttgattc ccaaacagaa tgagtcagag gtttttgaag atagacatgt
      601 ttacctaatt ccattgcggc tgcagcagtt acagcatgtg tatggtcagg tgtagcaatt
      661 acaactgcat caatgtcttt ttgtttctcc agcattaacc gaaaatcttt atatcgggaa
      721 gcctttggat aagatttgaa aacatccctg gcgtaattgt aatcacaatc gcatagagaa
      781 acaatgtttt gcccgaccat atttctcagg ttcacttttc ctttgccccc aataccgatt
      841 ccagcaatat taagcttatc gctcggagcg acgtgcccta atcccgagac agcataactt
      901 ggaagaatag ttaacgatac gccggttacg gccacattct ttaaaaaaat ccttcgtttc
      961 atctattaac taatttattt aattggccaa aaatataaaa aactgattat caaataatta
     1021 attggagttt tgcaaattta agcaaattac agtactttac cttttttcca cccagatagg
     1081 agaactccaa gcccattgct ggtttttttg tcttacgcgc acatagtagt aatcgatctc
     1141 tctttctgca acagtatctt ccatataatg ttctacctga aaagcgtttt cagggattgc
     1201 cctgccgaac aatattgctt cactcaacca gcctatcata aagtgtgatt ttgaaccttg
     1261 taataattct ccgatagaat gcgtgatctt tttcccattg aaatcggacg ttaagtttcc
     1321 cgctttaggc atggttacat ctaaaataac accctgaact gttggagtca tagtattggg
     1381 gttcttggtg ctatacacat ccagtattgt ttctttttca gaagaggata aaaccctgtt
     1441 tacccgggtc ttaaatgctt tctcgccagg ctggggagaa gtatatgccg ctcctctgaa
     1501 acagggcgtt atcctattta ttatcccttt atcaattgat aatctaccgt tccaatgtat
     1561 atattcttct tcacggttcc atccgaattc tacttttatt ttacatctca ccggatcttc
     1621 cttaggtacc gtgggaataa gcggcccgtt catgcgtgcg atgcatttac cgttcttgat
     1681 aatatctacg taatcaaccg aattctctcc ctctacattc aggtaaattc ttcgactgtt
     1741 tcctctgatg acttcgccca tgatggcgtc gttgatccga aaatctatct ttatcttgtc
     1801 acccgtggca catcctacat ttctgttctg caaagcattc cagatatcat cacgcttgag
     1861 agatggggcg agggccatca cccgtccgtc accatagctt ccgggatagc cggcatgctg
     1921 atctgtagag ccaataattc caaatttatg tccttgttcc aatccgtata acacggttcc
     1981 ttcccattgc ctgggcccca tgtcgtgaag atagttataa tcgccgtcat cgctttctgc
     2041 cagcccatgc cgggagtaca tttcaacgaa aggcgtctgt ccgtcttcct gaaatgactt
     2101 ccagttatac ccccgaaaac cggtctgata tcccatatgg tgaggggtaa caaatgcctt
     2161 ctgtcctttg agcctcgctt tcagctccat cgcatttgaa ggctccgcca ttgtcccatt
     2221 cacatcatat aataatgcta catgatcacc atgtgccatg tcgtgacatt cgtagctcaa
     2281 aaatgtaagg aactcatttt ctttgttgta tccgctcgtc attttcacgt attcgtccca
     2341 ttttcctgct cgcaatgcat cgaatgcttt agtgtgataa tcgataaccc attgcaggcg
     2401 catatcattc ctgccgggaa tatcttccca cattgcgtga ggggtgaccg aaacaaagtc
     2461 gagctgctcc ctggctgctt caaacgcatc tctcatatct ccgtgcccat aggtgagatt
     2521 gcagtggtta tgtatatcac cccaatagag tttcaggtta tgggaagatg gtaatgtttt
     2581 ttttgcctct ttggattttg gaggacaacc ttgcaacaag ctacctgttg cggctaaacc
     2641 aacaaatgtc catcccgtct tattgataaa ttctctcctg tccatctcgt aagaactaat
     2701 ccaatttcca aggttttcta tactcgtacg acaggtattt gttagcgacc tcagagttag
     2761 taaatctttc gtttaccgca tcccattcaa gcctttcccg tgtcatcata gcaattgttg
     2821 ccaaatgcgc catagtggtt gatctgtgtc caatctccaa ggtagcccaa ggttgttcgc
     2881 gtgattttac acaatcgagg aagttacgca caaggcttga cgcactgttt ttataactac
     2941 catcaattaa cagcgtgtca tctttagcca aagagtaatc ttcgggctcg atcattggct
     3001 tccatgtctg aaattgtccc gcactggcag gagtaatctt ataatcattt tctcctgtat
     3061 ggattgtccc ctttgtacct ctcagctcga gaaatccgtg gggaataaat gtgccggtac
     3121 ttgcttcaag tattgttaaa gtaacaagta ctcccgattt aaattcgtat gttacatgca
     3181 ttgtatctgg aatggtacga tcatcattca atacaaattt acctccatgt gcactgatgg
     3241 ccgttggagc ctcttcatct ataagccatc tcaacagatc gagataatga acaccgttat
     3301 tagaaatctg gtttgcatag tcctcccacc agcggaacat gtagggagca atgttatact
     3361 gatacggccg gtaagctctt ggacctaacc acatgtccca atcaaaattt cttggtgggg
     3421 tctcaggctg taatttccca atgccgctag gatacatgtt gcttacatga caagccgacg
     3481 caaaagttac ttttcctatt tttcctgcgg ggatttcttt tgctaatctc tgaaaagttg
     3541 gggcgccacg cctgtttaaa cccaccgtca cgatttgcct gctgctgttg ccaacttcaa
     3601 ccattcttcg gccttcagca attgtttttg ataaaggttt ttcaacaaat acatctttac
     3661 ccgcatttat agcatcaatt gtttgcaaag cgtgccagtg gtcgggtgtt gcaatacaaa
     3721 cagcatcaat acttttgtct cctaacatct cccgataatc tttgtactgc tttacgtttg
     3781 aagggaaatt ttctcccatc ttgggtattc tcgtccccat atctttcttg taacgatcga
     3841 gtacagcaga ataatctctt gtcacgtatg gctcatatac gtcacagaga gctgctactt
     3901 cacagtcagg ttcttccata aagaggtgta aaagttgtgt tccgcgatta cctactccaa
     3961 taaatcctac ccgaaccttg tcattagcac cgattactct tttctt
//
LOCUS       DAOSDR010000300         3991 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrimonas sp. isolate AMR_MDS_1826
            NODE_7961_length_3991_cov_3.998730, whole genome shotgun sequence.
ACCESSION   DAOSDR010000300 DAOSDR010000000
VERSION     DAOSDR010000300.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863355
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrimonas sp. (wastewater metagenome)
  ORGANISM  Petrimonas sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Dysgonomonadaceae; Petrimonas.
REFERENCE   1  (bases 1 to 3991)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3991)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:10:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,720
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 35
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3991
                     /organism="Petrimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7961_length_3991_cov_3.998730"
                     /isolate="AMR_MDS_1826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2023866"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(167..1366)
                     /locus_tag="PLI30_10790"
     CDS             complement(167..1366)
                     /locus_tag="PLI30_10790"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004293154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008776 - acetate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006085 - acetyl-CoA biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HOI80077.1"
                     /translation="MKILVLNCGSSSIKYKLFDMRSNEVIAQGGVEKIGMKGSFLKLT
                     LPDGQKVQLEGEILEHRAGIEYIFGVMLSEKYGCIRSLDEIDAVGHRVVHGGERFNKS
                     VLITEEVIEMLKECIELAPLHNPPNLKGIYAIQELLPHTPQVSVFDTAFHQTMPDYAY
                     VYGLPYSLYEKYGIRRYGFHGTSHRYVSKRACEFLNVPYESQRIITAHIGNGVSITAI
                     KNGKSVDTSMGMTPVEGLMMGTRSGDLDPGVISYIMEKEHMSASGISTLLNKFSGVLG
                     ISGISSDMREIEVGIKENNPRAILALNTYDYRIKKYIGAYSAVLGGVDILVFTGGVGE
                     NQAITRSVVCKNMEYMGIELDEELNRSVRAKEVVISKPSSKVKVLIIPTDEELTIAKD
                     TMQILGK"
     gene            complement(1395..2369)
                     /locus_tag="PLI30_10795"
     CDS             complement(1395..2369)
                     /locus_tag="PLI30_10795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019538738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase family protein"
                     /protein_id="HOI80078.1"
                     /translation="MSELIIEPIEKYGLNISHEKKALFSRIGVVGAGKEGRTIISLTA
                     SAGMDVVFMDESQERIDIVFEELNRVMDQKISKWGLTQSEKKVIMNRITPTLSYDDFT
                     GCDLVIECTRYSESGRRSTPVRKNIFKILDEILEPDAIIATNGPTVIISELAADLVHK
                     ERCVSLYFPVSHPDARILEIVKGMYTSEEVYNRMEIFAQLINYRPHRINESNGNVSIR
                     LLTTMLNESCQMLLERVSSMQSIEETFTIIYGQRYGVFRMADIIGIERIVTIMEAMFN
                     DFGETHYKPNPLLWKLYRSNQLGVRTSKGFYIYDENGNVISENKSLFN"
     gene            complement(2389..3402)
                     /gene="pta"
                     /locus_tag="PLI30_10800"
     CDS             complement(2389..3402)
                     /gene="pta"
                     /locus_tag="PLI30_10800"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009016811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008959 - phosphate acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HOI80079.1"
                     /translation="MNLINEIIERAKADKQRIVLPEGTETRTLQAADQLVRDRVAEII
                     LLGNPQEINLLANQLELKNIGKTLVIDPKNHDKKQTYIDLLVKLRQAKGMTPEKAAVL
                     VEDPLYLACLMIKNGDADGEIAGAQNTTGDVLRPALQIIKTSPGVSVVSGAFIMFTKT
                     TQYGKDGILIFADCAVMPNPTAQELASIAIASAQTARGIVGVEPRVAMLSFSTKGSAQ
                     HEMIDKVAEATRIAKEIAPDLLIDGELQADAALVPSVGASKAPGSAIAGQANVLVFPT
                     LEAGNIGYKLVQRLGNAEAVGPILQGMAAPVNDLSRGCSVDDIYKMVAIAANQSIAAK
                     SKK"
     gene            complement(3548..>3991)
                     /locus_tag="PLI30_10805"
     CDS             complement(3548..>3991)
                     /locus_tag="PLI30_10805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019538710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HOI80080.1"
                     /translation="KNPDVWELIRAHSNRLQSLPNEIALMTTNLPHGYHRDFQLLKET
                     LFPALEALHTLFEMTHFMLSHISVNKNILKDEKYRYLFTVEKVNELVLQGIPFREAYK
                     QIGQEVQEGTFAFQPVLNHTHAGSIGNLCTNEIRTKMENAMKGIC"
BASE COUNT          979 a          940 c          867 g         1205 t
ORIGIN      
        1 cccgtacgtt caaaaggcat acaggagtta ctccatcaca ttatcgcaat tttgttcaaa
       61 gaacctgaat cggttgtata aatataaact ctactctaaa atttcctatg taaaaacgcc
      121 gccaggtgga ggttatcctc tggcgacgtt ttttgatagt taaacgttac ttccccaata
      181 tctgcatagt gtcttttgcg atggtcaatt cttcgtcggt ggggatgatg agtactttca
      241 cttttgacga cggtttgctg atcacaactt ctttcgcacg aaccgatctg tttaattctt
      301 catcaagttc aatgcccata tactccatgt ttttacaaac gacactccgg gtaattgcct
      361 ggttttcacc aacgccaccc gtaaatacca agatgtccac tccgcctaaa acagcgctgt
      421 atgcacctat gtattttttt atgcggtagt cgtaagtgtt tagtgccaga atcgctctcg
      481 ggttgttttc ttttattccg acctctatct cacgcatatc cgatgagatt cctgagattc
      541 ccagaacacc cgaaaatttg ttcaacaggg tggaaatgcc tgaagcgctc atgtgttctt
      601 tttccataat ataggatata acccccggat ccaggtctcc tgaccgggtt cccatcatca
      661 gcccttcaac gggagtcatc cccatacttg tatccactga tttgccgttt tttatagccg
      721 ttatggaaac gccgttacca atatgggcag tgatgattcg ttgagattcg taggggacat
      781 tcaggaactc gcaggctctt tttgaaacgt accgatggct cgtgccgtga aatccatatc
      841 ggcgaatgcc gtatttttca taaagcgagt agggcaatcc gtaaacatag gcataatcgg
      901 gcatggtctg atggaaggcc gtatcaaaga cacttacctg tggcgtatgg ggcaacagtt
      961 cctgaatggc gtaaatacct ttgaggttag ggggattatg taagggcgcc aactcaatac
     1021 actctttcag catttctatg acctcttcgg tgatgagcac gctcttgttg aaacgttccc
     1081 cgccgtgtac cacgcggtgt ccgacagcat caatttcatc gagtgaccgg atgcatccgt
     1141 atttctcact caacatgaca ccgaaaatgt attcaatccc tgcacgatgc tccagaattt
     1201 ctccttcgag ctgaactttc tgtccatcgg gaagtgtgag tttcaagaat gagcctttca
     1261 tgccaatctt ttccacaccg ccttgtgcaa taacctcgtt gcttctcata tcgaaaagct
     1321 tgtattttat ggaagaactt ccgcaattta atactaatat cttcatttca acaacttatg
     1381 atttctgact tttctcaatt aaacaacgat ttattttcgg aaattacgtt tccgttctca
     1441 tcatagatgt agaagccctt acttgtccga acgccaagct gattggaacg gtaaagtttc
     1501 cacaacaacg gattgggttt gtaatgtgtc tctccaaaat cgttaaacat ggcttccatt
     1561 atagtgacaa tacgttcaat accaatgatg tccgccattc tgaaaacacc gtaccgctgt
     1621 ccgtaaatga tggtaaacgt ttcttcaatg ctctgcatgc tgctcaccct ctccaacaac
     1681 atctggcacg attcgttgag cattgtcgtc agtaagcgta tgcttacatt accgttgctt
     1741 tcatttatcc ggtgaggccg gtaattgata agttgggcga aaatttccat tctattgtac
     1801 acctcttccg atgtgtacat tccctttacg atctcgagaa tccgggcatc gggatgagat
     1861 acgggaaaat agaggctgac gcagcgctct ttatgaacaa ggtcagctgc caattcactg
     1921 ataatgacgg tagggccgtt agtagcgata atggcatcgg gttccaggat ctcatccagt
     1981 attttaaaaa tattctttcg aacgggtgtg ctgcggcgtc ccgattctga atatcgggta
     2041 cactcaatga ccaggtcgca tccggtaaaa tcatcgtagc tcagtgtggg agtgatccgg
     2101 ttcatgataa cctttttctc cgactgcgtc aatccccatt tgctgatttt ctgatccatt
     2161 acccggttca gttcttcaaa aacgatgtcg atacgttctt gagattcatc cataaaaacc
     2221 acatccatcc ctgccgaagc tgtcaggctg attatggttc gcccttcttt tccggcccct
     2281 acaacaccta ttctagagaa gagcgctttc ttctcatggc ttatgttgag tccgtacttc
     2341 tcgataggtt caatgattaa ttcactcatt attctatttt tttgaattct atttttttga
     2401 ttttgcagca atggactgat tagctgctat ggcaaccatt ttgtaaatgt catcaaccga
     2461 acatccgcgg gataaatcgt ttaccggagc tgccatccct tgtaaaatgg ggccaaccgc
     2521 ttcggcattg cctaaccgct ggacaagttt gtatccgata ttcccggctt ccaacgtggg
     2581 aaaaaccaat acgttggcct gccctgcaat cgcacttccg ggagctttgc ttgcaccaac
     2641 agatggaacc agcgcagcat cggcctggag ctcaccgtca atgagcagat cgggagcgat
     2701 ctctttggca atgcgggtag cttccgccac cttatcaatc atttcgtgct gggcacttcc
     2761 tttggtcgaa aaacttaaca tagccacgcg cggttcaacc ccaacaatac cgcgtgcggt
     2821 ttgtgccgat gctatggcaa tggatgccag ctcctgtgca gtaggattgg gcatgacggc
     2881 acaatcggca aaaataagga tgccgtcttt cccgtattgc gttgttttcg taaacataat
     2941 aaatgctccg gatactacgc ttactcccgg tgatgttttg atgatctgca aagccgggcg
     3001 gagcacatcg ccagtagtat tttgtgcgcc ggcaatttcg ccgtccgcat caccgttttt
     3061 aatcatcagg catgctagat agagcgggtc ttccaccaaa acggccgctt tctcgggtgt
     3121 cattcctttg gcctgccgga gtttcaccaa caggtcaatg taggtttgtt ttttgtcgtg
     3181 gttcttagga tcgattacga gggttttccc gatatttttc aattcaagct gattagccag
     3241 caagttaatt tcttgcggat ttccgagaag aattatttct gccaccctat cccggacaag
     3301 ctggtccgca gcctgaagag ttcgagtttc cgtcccttca gggagaacga ttcgttgttt
     3361 atcggctttt gcacgctcga taatttcgtt aattaagttc atagattttg tatgacgatt
     3421 gagtttttgg aataagatat ccgggaggta aatcctgttc gatctaccgc ccgtttcaat
     3481 ttgaaattaa tcttctgcaa aagtaataaa aaaagcgata tgaggcttgc aaatggttag
     3541 gagaaaatta gcagattcct ttcattgcgt tttccatttt tgttcgaatc tcattcgtac
     3601 ataaatttcc aatgcttccc gcgtgtgtgt ggttaagaac aggttgaaag gcaaaagtac
     3661 cttcctgcac ctcctgcccg atttgtttgt aggcttcacg aaacggaata ccctgcagga
     3721 caagttcgtt gactttttcc accgtgaaga ggtagcggta tttttcgtct ttcaaaatat
     3781 tcttgtttac tgaaatgtgt gaaagcataa aatgagtcat ttcgaagagc gtgtgcaacg
     3841 cttcaagtgc cggaaaaaga gtttctttca gcaattggaa gtcacgatgg tatccgtggg
     3901 gcaggttggt ggtcatcagg gcgatttcgt tgggaagact ttgtaaccga ttgctgtgcg
     3961 cgcggataag ctcccagacg tcggggttct t
//
LOCUS       DAOSDR010000301         3990 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrimonas sp. isolate AMR_MDS_1826
            NODE_7963_length_3990_cov_4.717408, whole genome shotgun sequence.
ACCESSION   DAOSDR010000301 DAOSDR010000000
VERSION     DAOSDR010000301.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863355
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrimonas sp. (wastewater metagenome)
  ORGANISM  Petrimonas sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Dysgonomonadaceae; Petrimonas.
REFERENCE   1  (bases 1 to 3990)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3990)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:10:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,720
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 35
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3990
                     /organism="Petrimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7963_length_3990_cov_4.717408"
                     /isolate="AMR_MDS_1826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2023866"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..262
                     /locus_tag="PLI30_10810"
     CDS             <1..262
                     /locus_tag="PLI30_10810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006843282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HOI80081.1"
                     /translation="VGSYDSLTIEFARKMDAKFILRGIRSVNDFEYEKTIADMNRMIS
                     GIETFVLFTEPELTHISSSHVRELLRYGHDVSAFVPKGMILG"
     gene            276..1889
                     /locus_tag="PLI30_10815"
     CDS             276..1889
                     /locus_tag="PLI30_10815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018074904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HOI80082.1"
                     /translation="MSKTTFFLATLLLVSSLSFGQSRLSPDGVKLERTLQMINGLYVD
                     DVETSHLTEAAIRAMLLELDPHSTYLNKDEVKAMNEPLQGNFDGIGISFNMLTDTLYV
                     MEVISGGPSQKVGLLPGDKIIYVNDSLIAGIKKDNQDVIKMLRGPKGTTVNVRVLRRG
                     TPQLLDFRIVRDKIPIYSIDAKYMVDHHTGYIRLSRFGVSSSEEFLAAEKELKAKGMK
                     NLILDLTGNGGGILQTASDIVDEFLGAGKLVVYTEGKNQPRYDLRTTSAGSFEQGSLV
                     VLVDEGSASASEIVAGAVQDWDRGVVVGRRTFGKGLVQRQLPLPDGTMIRLTVARYYT
                     PTGRSIQKPYEEGNIDAYNRDFFNRVQHGEMWHADSIRFPDSLRFKTLVNKRTVYGGG
                     GIMPDYFVPVDTNSVTDLHRNLIGRGIVNKLAIAEVDKNRARLLKSYSHVEKFKSDYR
                     IPESMITRMKEMAKGEKMEWDDEQFRKSEQFLKIQLKALMARDLFDSSAYFVIINDEN
                     DLFREGLRIISNPGVYDELLNGSFSVARK"
     gene            2009..3244
                     /locus_tag="PLI30_10820"
     CDS             2009..3244
                     /locus_tag="PLI30_10820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019541213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1080 domain-containing protein"
                     /protein_id="HOI80083.1"
                     /translation="MTMVFASCEPSAKHNVLTEAEKAEGWQLLFDGETLNGWRDYNGD
                     SLTAPWFAEDGMIQAKGEGADEHGYIVTDKLYENFELVWDWKVADGGNSGVLYHVVEN
                     PKFTVPYVTGPEYQLIDNLGFPDPLEEWQKTAADYAMYVPDTARLIIKPAGEWNTSKI
                     VFDNGHVEHWLNGEKVIEFEAWTEDWFARKNSGKWENAPEYGLARKGVICLQDHGSAA
                     WFRNVKIKELPRKTQEVNLFNGVDLTGWEAYGTEKWYVQDGLLVCESGPDKQYGYLAT
                     REYYDNFDLSVEFKQEADGNSGVFIRSFIEPGAIVNGWQVEVAPKGNDTGGIYESYGR
                     GWLVQIPDEKEEILKVGDWNTLRIVINGDNVKTYLNGEEMVDLTDEKIGKAQGRIALQ
                     IHDGGGIKVLWRNLKLQTL"
     gene            complement(3345..>3990)
                     /locus_tag="PLI30_10825"
     CDS             complement(3345..>3990)
                     /locus_tag="PLI30_10825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019541239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HOI80084.1"
                     /translation="TGIDGIEVNLYQTPVDFSSSSESVENNQIAIVEEVKKAVSVPVS
                     VKLSSDYTNILHFAKRLDEAKVGSLVLFNAFFQPDIDIQKEKHKKVFNLTQKGDYKKT
                     LRYTGMLYGNIKADICSSRGVFTGEDVVKLILSGATTVQVVSSVYKHGVERIASIKNG
                     ITEWMQQKGYNNLDEFRGKLANVNLDKNEHSLVYKRAQYVDLMLTSETIFGNFQ"
BASE COUNT         1124 a          831 c          972 g         1063 t
ORIGIN      
        1 agttgggagc tacgattctc tcactatcga gtttgcccgg aaaatggatg caaagttcat
       61 attacgggga atacgctcgg ttaatgattt cgagtacgag aaaaccatag ccgatatgaa
      121 ccggatgatt tcgggaatag aaacatttgt attatttacc gagcctgaac ttacgcacat
      181 cagttcctcc cacgtacgtg aattattgcg ttacggacac gatgtgagcg cttttgttcc
      241 gaaagggatg atacttggat aacaattgaa taacaatgtc taaaacaact ttttttcttg
      301 caacactttt actggtatcc tccttgtcgt ttggccaatc tcgtttatcc cctgatggag
      361 taaagctgga gaggacgctc cagatgataa acggtttata cgtcgatgac gttgaaacct
      421 ctcatctgac agaagcggcc atccgtgcca tgttgcttga actcgaccca cactccactt
      481 acctcaacaa ggatgaggtg aaagcgatga acgaaccgtt acaaggcaat tttgacggta
      541 ttggaatctc gttcaatatg cttaccgata cactttacgt aatggaagtc atttcaggcg
      601 gaccttcaca aaaggtgggt ctcctgcccg gcgataaaat tatttacgta aatgattcgc
      661 ttattgccgg tataaaaaag gataaccagg atgttataaa gatgctccga ggtcccaaag
      721 gcacaaccgt gaacgtgagg gttttgcgtc gtggaactcc gcagttgctg gacttcagaa
      781 tcgttcgcga caagatcccc atttacagta tcgatgccaa atacatggtg gatcatcata
      841 ccggatacat tcgtttaagc cgctttgggg tcagttcatc cgaagaattt ctggcagcag
      901 agaaagaact gaaagcaaaa ggaatgaaaa accttattct cgacctgacc ggaaacggag
      961 gaggtatttt gcaaacggca tcggatattg tggatgaatt cctgggtgcc gggaaattgg
     1021 tggtatatac cgaagggaag aaccagcctc ggtatgattt acgcacaacg tcggcaggaa
     1081 gctttgaaca ggggagcctg gttgttctcg tggatgaagg ttcggcgtcg gcaagtgaaa
     1141 tagtggccgg ggccgttcag gattgggatc ggggtgtagt ggttggccgc cggactttcg
     1201 gaaaggggtt ggtgcaacgt caacttccgc ttcccgacgg aacgatgatc cggttgaccg
     1261 ttgcccggta ttacacacct acgggcagaa gcatccagaa gccttacgaa gaaggcaata
     1321 tcgacgcata caaccgtgat ttcttcaacc gggtacaaca cggtgaaatg tggcacgccg
     1381 acagtatccg tttccccgat tcgttaaggt tcaagacgct tgtcaacaag cgcactgttt
     1441 acggtggagg tgggatcatg cccgactatt ttgtacctgt ggacacgaac tcggtaacgg
     1501 atttacaccg taaccttatt ggccggggaa tagtcaacaa actcgctatt gccgaagtgg
     1561 acaaaaaccg ggcaagattg ttaaaatcat attcccacgt cgaaaaattc aagtcggatt
     1621 accggattcc cgaatccatg ataacccgga tgaaggaaat ggcaaaagga gaaaaaatgg
     1681 aatgggacga tgagcaattc cggaagtcgg aacaattttt gaaaatacag ctcaaggcac
     1741 tgatggctag ggatctgttt gattcatccg cctattttgt tatcatcaat gacgaaaatg
     1801 atcttttcag ggaaggcttg cgtatcatca gcaatcccgg ggtttatgac gaactcttaa
     1861 acggatcgtt ctctgtagcc cgaaagtaac cggcgacggg aacagttgta taattttcat
     1921 aaaatagata cttttgtttt cctaacgttt aattataaaa tcagaaaaaa tgaaatcaag
     1981 atttttttta ttgtgcacaa cactgcttat gacaatggta tttgcctctt gtgaaccttc
     2041 cgcgaaacac aatgtattga cggaagctga aaaagccgaa ggctggcagc tccttttcga
     2101 tggagaaacc ctgaacggtt ggcgcgatta caacggtgat tcattaaccg ctccgtggtt
     2161 tgccgaagac ggtatgattc aggcaaaagg cgagggcgct gatgaacacg gttatattgt
     2221 aacggataaa ttgtatgaaa actttgaatt ggtgtgggac tggaaagtcg ccgacggtgg
     2281 aaacagtggg gtgctgtacc acgtggttga aaatcccaaa tttacagtac cgtacgttac
     2341 gggaccggaa tatcagttga ttgataatct tggatttccc gacccgctgg aagaatggca
     2401 gaaaacggct gctgattatg ccatgtatgt tcccgatacg gcgagactta ttattaaacc
     2461 ggccggagaa tggaacacat cgaaaatcgt cttcgataac ggacatgttg agcattggct
     2521 gaacggtgaa aaggtgatcg aattcgaagc atggacagaa gattggtttg cacgcaaaaa
     2581 cagcggaaaa tgggaaaatg cacccgaata cggcctggct cgtaaaggtg ttatctgttt
     2641 gcaagaccac ggatcggcag cctggttccg caacgtgaaa atcaaagaac ttccccgtaa
     2701 aacccaggaa gtaaatcttt tcaacggtgt tgacctgact ggatgggagg cttacggaac
     2761 cgaaaaatgg tatgttcagg acggattgtt ggtttgtgaa agcgggcctg ataaacaata
     2821 tggctatctg gctactcgtg agtattacga caatttcgac ctttcggtag aatttaaaca
     2881 ggaagccgat ggaaactcgg gagttttcat tcgttcattt attgaaccgg gggctattgt
     2941 gaatggctgg caagttgaag ttgctcccaa aggaaatgat acaggaggca tttatgagtc
     3001 ttacggaagg ggttggctcg tacagatccc tgacgaaaaa gaggagatcc tgaaagtggg
     3061 cgattggaac accttgcgta ttgttattaa tggtgataac gtgaaaactt atctcaacgg
     3121 tgaggaaatg gtggatctga ccgacgagaa aatcggaaag gctcagggtc gtattgcctt
     3181 gcaaatccac gatggcggtg gtatcaaagt gctttggaga aatttgaagt tgcagacgct
     3241 gtaatccgac ctgtaatccg gccttacgtc ttgttacgct ggaaataaac aaaacccact
     3301 gagccctttt cgcttagtgg gttttgtaat atgaatacag gggattattg aaaatttccg
     3361 aaaatagttt ccgaagtcag catcaaatca acatattgcg cccttttata aaccagtgaa
     3421 tgttcgtttt tgtctaaatt gacattggcc agttttcccc tgaactcatc aaggttgttg
     3481 tatcccttct gttgcatcca ctctgttatg ccgttcttta tggaggcaat tctttctacg
     3541 ccgtgtttat ataccgagct gacaacctgc actgttgttg cacccgacag gatcagcttc
     3601 accacatctt ctccggtgaa aactccccgg ctactgcaaa tatcagcttt aatattgccg
     3661 tacaacattc cagtatacct cagcgttttt ttgtaatcgc ctttttgggt aaggttaaaa
     3721 acctttttat gcttttcctt ctgaatatca atatcgggtt ggaaaaatgc attaaaaagg
     3781 actaaggaac ctacctttgc ttcgtccaac cttttcgcga aatgaaggat gttcgtataa
     3841 tccgaactca acttcacgct tacgggaacg gacacggctt tctttacctc ttcaacgata
     3901 gctatttgat tgttttcaac actttcactg ctgctactaa aatcgacggg ggtctggtaa
     3961 agatttactt caatcccgtc aatcccggtt
//
LOCUS       DAOSDR010000302         3960 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrimonas sp. isolate AMR_MDS_1826
            NODE_8048_length_3960_cov_3.979770, whole genome shotgun sequence.
ACCESSION   DAOSDR010000302 DAOSDR010000000
VERSION     DAOSDR010000302.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863355
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrimonas sp. (wastewater metagenome)
  ORGANISM  Petrimonas sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Dysgonomonadaceae; Petrimonas.
REFERENCE   1  (bases 1 to 3960)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3960)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:10:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,720
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 35
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3960
                     /organism="Petrimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8048_length_3960_cov_3.979770"
                     /isolate="AMR_MDS_1826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2023866"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1700)
                     /locus_tag="PLI30_10830"
     CDS             complement(<1..1700)
                     /locus_tag="PLI30_10830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019539573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HOI80085.1"
                     /translation="MKSNQILTIKNTLTLLFGILVLSISAQNNTIRGTVTYATSGDPV
                     IGATVRLQSATGSGTVTDVDGNYVLNNAPSNGTLEFSYVGMKTQSVQVNGRTVIDIVM
                     AEDSELLDELVVTALGIKRDKKALGYAVQGVSSDVFKTRPTNTLSALAGVVSGLQVIS
                     SGGNMGGSSRVTLRGINSITGNNQPLYVIDGVPLDNTDLNTSLTINGSAGKDVGSTIQ
                     DINPDDVAEVSVLKGPSAAALYGSRAANGVILITTKRGTTEQGKINVELNTGIEFENI
                     ARLPERQKLYGGGYNTTFSTATIDGREYKIVDYASDESWGPKLDGTPVLHWYNLDPEY
                     PSDYLKPEPWVYPKHDVTYFFKTGVSNTNNISLSQSSDKHSIRLSLTNRNVTGTVPNS
                     SLDRNSVNISGSTNGKYVSFFGTFNYLKNKSLGRPWTGASNRNIILEAYQWGMVQIDY
                     KKLQEYKRPDGTPRAWNRTGYRNTVEDERTKYIDNPYWSAYESYLEEDRDRLYGNVGI
                     EITPSDWLSVTGRINGDVYQYNSQDRIAVYSRSQSSYSEYSQKYNEFNYELLATITKN
                     "
     gene            complement(2306..2377)
                     /locus_tag="PLI30_10835"
     tRNA            complement(2306..2377)
                     /locus_tag="PLI30_10835"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2342..2344),aa:Arg,seq:ccg)
     gene            complement(2383..2997)
                     /locus_tag="PLI30_10840"
     CDS             complement(2383..2997)
                     /locus_tag="PLI30_10840"
                     /inference="COORDINATES: protein motif:HMM:NF023074.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3256 family protein"
                     /protein_id="HOI80086.1"
                     /translation="MKKIVTVLFIFIATSAFPQKIDDVFKTMPNSILPGLSDGNRTML
                     LVDTGKTVIPYSLGEIEKLAYAPDFLKIKTSGIGSTQLKLLPLINDTQIICVIRTVCG
                     KACDSNIRFYSTDWKELEAKTLLSHISAASFFDSSKKDSENYKFALSLPDIYPVSAEF
                     ENGSNALTLKLDLEGYLSDEQLAEVKPFIKSETITLNWNNISFR"
     gene            complement(3004..3891)
                     /locus_tag="PLI30_10845"
     CDS             complement(3004..3891)
                     /locus_tag="PLI30_10845"
                     /inference="COORDINATES: protein motif:HMM:NF012956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="discoidin domain-containing protein"
                     /protein_id="HOI80087.1"
                     /translation="MDVSIRPGWFYHPEQDHQVKTLQQLVDIYYQSVGMNSVLLLNIP
                     PDKRGLLHEADVARLKEFGAYIRSTFENNRINTGNTAWTANNGEFREFTVTGDTVNTV
                     MLQEDIKKGQRVEVFKVEGFIHNEWKKLAEGTTIGYKRLLKFDDCAPSRIRVTVTETR
                     EKAHILAVGAYRAPQISETSGELKLSDVSKEKWTVKSHSPLIVDMGEVVSVKGFTYKP
                     ISEKEAVFNYAFSMSNDGKTWTGLKKGEFSNIKNNPIPQFVRFDNDLKARFFRFETII
                     GTEGGKPGVSIDQIGILTQ"
BASE COUNT         1057 a          823 c          803 g         1277 t
ORIGIN      
        1 cagttttttg ttattgtagc caataattca taattaaact cgttgtactt ttgactgtac
       61 tcagaatagg atgattgaga acgcgaataa acggcgatac ggtcttgtga gttgtattga
      121 tatacgtcgc cattaattcg tccggtaaca gataaccaat ccgagggcgt aatttcgatc
      181 cctacattac cgtacaacct gtcgcgatct tcttccagat aactttcata tgccgaccaa
      241 tacggattat cgatatactt tgtccgttca tcttcaacgg tgtttctgta tccggttcgg
      301 ttccatgcac gaggtgtgcc atcgggacgc ttatattcct gtaacttttt ataatcaatt
      361 tgtaccattc cccactggta tgcttcaagt atgatgtttc ggttggaagc tcctgtccac
      421 ggacgcccca gtgacttgtt ttttaaataa ttaaaagtac caaagaacga cacatacttt
      481 ccattggttg aacccgaaat atttaccgag tttctgtcta aagatgaatt gggaaccgtt
      541 ccggttacat tcctgtttgt caacgacaaa cgaatggagt gcttgtcgga agactgtgac
      601 agtgaaatgt tgttggtatt ggaaacgccg gttttaaaga aatacgtcac atcgtgcttt
      661 ggataaaccc aaggttcagg ttttaaataa tcgcttggat attccggatc aagattatac
      721 caatgcaata caggcgtacc gtctaatttt ggcccccagc tttcgtctga agcataatcc
      781 acaattttgt attcccgccc atcaatcgtt gctgtagaaa aagttgtatt gtaacctcct
      841 ccgtaaagct tctggcgctc gggcaaacgt gcgatattct cgaattctat tccggtgttc
      901 agttcaacat ttatcttccc ttgttcggtt gttcctcttt tggtagtaat gagtattact
      961 ccgttagcag ctctggaacc gtacaaggcc gcagcggaag gccctttcag tacgcttact
     1021 tcggcaacat catcgggatt gatatcctga atggtacttc ccacatcttt tcccgcgctt
     1081 ccgttaatgg ttaacgaggt gttcaaatcg gtattgtcga gcggtacgcc atcaataaca
     1141 taaagaggct ggttgttacc cgtaattgag ttgattcctc gcaatgtaac gcgcgacgat
     1201 ccacccatat tgcctccgct tgaaattacc tgaagaccgg atacaacacc tgccagtgcg
     1261 ctcaacgtat tggtagggcg tgttttgaac acatcgcttg atacaccctg gactgcatat
     1321 cccaaagctt ttttatctct tttgatcccc agcgcagtaa ccaccagttc gtccaataat
     1381 tcactgtctt ctgccatgac aatatcaata actgtacggc cattgacttg aaccgactgc
     1441 gttttcattc caacataact aaactctaat gttccgtttg aaggagcatt gttcaataca
     1501 taatttccat caacatcggt taccgtacct gagcctgttg cgctttgtaa acgcacagta
     1561 gccccaataa cgggatcacc ggatgttgcg tacgttacag tacctcttat cgtgttattc
     1621 tgagccgaaa tagataagac aagaatacca aacaacaacg taagggtatt cttgattgta
     1681 agaatttgat tgcttttcat cctttttgtt ttaaatgatt tttaaaatgt tttattaaat
     1741 acaatgtttc ctataagctt acattacaat tattaagatt ttatcatcca tatatcttta
     1801 attttaattt atttatgttt tattttttta gtaattacaa tgataaatac ttctataata
     1861 gatatttatc aataaattat gcctgattag ggcacgacaa agtttgagaa taagaaaggg
     1921 gtaggggaga attgtcagag acacattcgg gagaatacag ataacctaca acaacagcac
     1981 gaagtttcgt gcaacttgaa cgttttttta ttgttgattt gatggatatt cacgatcatc
     2041 tgtttttttg acaatacaaa ggtaaaacat tttttttatt tacaaaatat cattgatata
     2101 atgtttttgg tgtcaattgt gtattttttc tttaggggaa tttttttaac ttatgagtct
     2161 aaaaaaaata ttgttgttgt tgaagtattt cacacgaaga aaacgattgt gtagctgtgc
     2221 aaataaattc gtggtatcaa tcgtggatat gtttaaaggg ttagtggcaa taggttgtat
     2281 atcagtgatg tatttttttt gagagtacac ccggcgagat tcgaactcgc atcgtcggaa
     2341 ccggaatccg atattctatc cattgaacta cgggtgcaga aattacctga aagaaatatt
     2401 gttccagttc agcgtgattg tttcactttt tataaacggc ttaacctctg ctagttgctc
     2461 atcagaaagg tatccctcca gatcgagttt cagtgttaac gcgtttgagc cgttttcaaa
     2521 ttcagccgaa acgggataaa tatccggcaa agatagtgca aatttataat tttcggaatc
     2581 tttttttgat gaatcgaaaa aagatgctgc agaaatatgc gataaaagcg tttttgcttc
     2641 caattctttc caatcggtag agtaaaaccg gatgttgctg tcgcatgctt ttccacaaac
     2701 ggttctaatg acgcaaataa tctgggtgtc gttgatcagc ggtaatagtt tcagttgcgt
     2761 tgagccaatc ccggaagttt ttatttttaa aaaatccggt gcataagcca gtttctctat
     2821 ttccccaagg gagtagggga tgaccgtttt tccggtatcc accagaagca tggtcctgtt
     2881 gccgtccgat aatcccggta agatggagtt gggcattgtt ttaaaaacat cgtctatttt
     2941 ctgcggaaat gcagaggtag cgataaaaat aaataatacg gtaactattt ttttcattgg
     3001 atgttattga gtgagaatac ctatttgatc aatggataca cctggttttc caccttctgt
     3061 cccgataatg gtttcgaacc ggaaaaacct tgccttcagg tcattatcga accttacaaa
     3121 ttgtggaata ggattgtttt tgatgttgct gaactcacct tttttcaatc cggtccatgt
     3181 ctttccatcg ttgctcatgc tgaaagcata gttgaagacc gcttcttttt cactgatggg
     3241 tttgtaggtg aagcctttaa cggaaacaac ctcgcccata tccacaatca acgggctgtg
     3301 gcttttgacg gtccattttt ctttggacac gtcgctcagc ttcaattcgc cggatgtttc
     3361 tgaaatctga ggggcgcgat aagcacctac ggcaaggata tgagcttttt cgcgggtctc
     3421 ggtgacggtt acccggatcc tggaaggagc gcaatcatca aatttaagca atcgcttgta
     3481 tccgatggta gtcccttctg ccagtttttt ccattcattg tgaataaatc cttccacttt
     3541 aaacacctcc acgcgttgac cttttttaat gtcttcctgc aacatcacgg tgttgacggt
     3601 gtcgccggtt acggtaaatt ccctgaattc tccgttgttg gcggtccagg cagtgtttcc
     3661 ggtatttatc ctgttgtttt caaaagtact cctgatgtat gcgccaaatt ctttcagcct
     3721 agccacatcc gcttcgtgca gtaagccgcg tttgtcggga ggaatattca gcaggaggac
     3781 cgaattcatc ccaaccgatt gataatagat atcgaccagc tgctgcagcg tcttcacctg
     3841 gtggtcctgc tcaggatgat aaaaccatcc cggacgaatg gaaacatcca cttccgaagg
     3901 gtaccaatag agttttttag ctcgccaata aggttgcggc tacctaaatc cttggacatg
//
LOCUS       DAOSDR010000303         3935 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrimonas sp. isolate AMR_MDS_1826
            NODE_8112_length_3935_cov_4.054897, whole genome shotgun sequence.
ACCESSION   DAOSDR010000303 DAOSDR010000000
VERSION     DAOSDR010000303.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863355
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrimonas sp. (wastewater metagenome)
  ORGANISM  Petrimonas sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Dysgonomonadaceae; Petrimonas.
REFERENCE   1  (bases 1 to 3935)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3935)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:10:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,720
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 35
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3935
                     /organism="Petrimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8112_length_3935_cov_4.054897"
                     /isolate="AMR_MDS_1826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2023866"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            38..958
                     /locus_tag="PLI30_10850"
     CDS             38..958
                     /locus_tag="PLI30_10850"
                     /inference="COORDINATES: protein motif:HMM:NF016895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HOI80088.1"
                     /translation="MNQLISHIEYLLHTHNCVIVPSLGGFVVNTAPAHRNGLSVFNPP
                     ECELVFNRELSHNDGLLVESYMRIDGISFENATQKINDSVRQIKNQLRSEKSVDLGDL
                     GSFRMVDDQRFVYQSKNFVRPEHFGLSSASLKPVIQLQPAAQLVEIPESSKKSIVRKA
                     AIGAAAAAVIAAIMLLFPIQDSTLRHQTAQFFSLGSVPSVAAGVVSGETPDLSENITT
                     TQPEVEATLSSEGPKFYIVTGVYQVRNVADDMIALLHSEGFSAASVIDRPNRIDVYAL
                     SFSTREEAEQNLKQLKKDFPNHRDAWVLKR"
     gene            complement(963..2462)
                     /locus_tag="PLI30_10855"
     CDS             complement(963..2462)
                     /locus_tag="PLI30_10855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007563741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HOI80089.1"
                     /translation="MHIIRKVCFLVLLIGSLPVGAQNKVSKKIMEIGKTDNRTMQHLD
                     ILTNRIGGRPIGSDAYENATYWVAGKLKEWGLEVEIQEVGEMPVGFNRGPWFGKMYSD
                     TSMSLDFVTPSYTSGTKGVQRGHVVVEPKTRQEFERMKGKLKGAWVLIEGQNTGFPID
                     HSVSGDSLRSAIIAKKEASPNDSDKITEPALFYKEMREAGILGTIQSAPVPLVAMYDR
                     KNIYSLSFDNLPTIPDIKLNEDQYNIIRKKVERREFVMLEFDIRNHFKPGPVKYHNVI
                     GKIRGTKYPDEYVMSGCHLDSYDSSTGAVDCGTGVAPNLEMARMIMAAGGNRPDRTIL
                     FCFWAGEEFGLLGSKFWVENNPDKLKKISNYFNRDGGPTAASGLNVPENMYDDIVKCT
                     EGLADYNPQIPFTVEKSTQPPRAVPITAGGSDHAYFAMNGVPTLAFSNTDPLGYNFSY
                     REIWHTTRDLYNLSIPEYMDYTSVTQAVTVYNMANLKNLLPRDGIYIQE"
     gene            2710..3603
                     /locus_tag="PLI30_10860"
     CDS             2710..3603
                     /locus_tag="PLI30_10860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI80090.1"
                     /translation="MKIPQQLLLLFFLMFLVFYSVCAQENPIRKDSLRIIPKNPLSLP
                     SDSAAPGGVSVIIPTIHPIVGKDGLQYNRFNDFTVNPYLMSRTQPSGVQFYGTGSDNI
                     NSKSRTAVATYSPVPRISLYSAATLGLVETPFFGKGNYYILNAGASYLLTPNLNAGLS
                     GMYNSNFNVIPFWSVSADLQYLAGRNLMLEGSAGFMKTGANMFNLNQSAVLIDLHARY
                     RLSDDWFLNAYGGFPVTQKTNRPGVPMMPMMDNTHYGGTVEYWFQPTVGAEAGLIWVR
                     DMFSGKMRPQPKLELKFRPGR"
     gene            complement(3627..>3935)
                     /locus_tag="PLI30_10865"
     CDS             complement(3627..>3935)
                     /locus_tag="PLI30_10865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019540673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative sulfate exporter family transporter"
                     /protein_id="HOI80091.1"
                     /translation="SNIRPSGSVLWERFPKFVLGFLVASLIFSFAFEPATGKSLSKIA
                     QESRGLLFSLAFVCIGLETSFRDIFKKENSKYIWSFLIAQGFNIVLTLVVAWFLFRSF
                     "
BASE COUNT         1052 a          964 c          830 g         1089 t
ORIGIN      
        1 attttcaaat ccataatcat ttttttatct ttgcgttatg aaccagctga tttcccatat
       61 agaatatctt ttacatacac acaattgtgt aattgttcct tcactgggag gatttgttgt
      121 gaatacggct cctgcccaca gaaacggact ctcggttttc aatccaccgg agtgtgaact
      181 ggtatttaat cgagagctct cccacaacga cggattattg gtggagtctt acatgcgtat
      241 tgacggcatt tcttttgaaa acgccacaca aaaaatcaat gactccgtgc gacagattaa
      301 aaaccagtta cgttctgaga aatctgtcga tctgggcgat ctgggaagtt tccggatggt
      361 ggatgatcag cggtttgttt atcagtcaaa aaattttgtg cgacccgaac actttgggct
      421 ttccagtgct tccttgaaac ccgtaattca gctgcagccg gctgcacagt tagtcgaaat
      481 accggaaagc agcaagaaat ccattgtaag gaaagcagcc attggtgcag ctgcagcagc
      541 ggttattgcc gctataatgc ttcttttccc cattcaggac agtactcttc gccatcaaac
      601 ggctcaattc ttctccctcg gttcagtccc atccgttgcg gctggagttg tttccgggga
      661 aacgccggat cttagtgaaa acattaccac aacccaaccc gaagtagaag ctacgctgtc
      721 atcggaaggc cccaagtttt acatcgtgac cggtgtttat caggttcgca atgtagccga
      781 tgacatgatt gccctgttgc attccgaagg attttcagct gcttccgtta tcgatcgccc
      841 caatcgtatc gatgtttacg ccttgtcttt ctctacacgc gaggaagctg aacaaaacct
      901 gaaacagctc aaaaaggatt ttcccaatca ccgggatgcc tgggtattga aaaggtgatc
      961 gtttactcct gaatataaat cccgtcccgc ggtaacaaat tctttaaatt agccatgtta
     1021 taaactgtaa ccgcctgcgt tacggatgta tagtccatgt actcaggaat gcttaagtta
     1081 tacaggtcgc gggtagtatg ccagatttcc cggtaactga aattataccc taacggatcc
     1141 gtattgctga aagcaagagt cggaacgcca ttcatagcaa aataggcatg atcgctacct
     1201 ccggcagtaa tggggacagc acgaggaggt tgtgtgcttt tttcaacggt gaaaggaatc
     1261 tgaggattgt aatcggctaa cccctccgta cattttacaa tatcgtcgta catattttcg
     1321 ggaacgttca accccgatgc ggcggttgga ccgccgtcac ggttaaaata attggatatc
     1381 tttttcaatt tatccggatt gttctccacc caaaatttag agcccaacaa cccaaactct
     1441 tctcctgccc aaaaacaaaa caaaatcgtc ctgtcgggac ggtttcctcc ggcagccatg
     1501 atcatccgcg ccatttccag attaggagca acgcccgtcc cacaatcaac cgccccagtg
     1561 gaggaatcgt aggagtccag atgacagccg ctcatcacgt attcatccgg atatttcgtc
     1621 ccccggatct ttcctatcac attgtggtat tttaccggtc cgggtttgaa atggttgcgt
     1681 atgtcaaatt ccaacattac aaactccctc ctttccactt tttttcgaat aatattatac
     1741 tgatcttcat tcaattttat atcgggaatg gtgggcaggt tatcaaacga caagctgtaa
     1801 atatttttcc ggtcatacat cgccaccagt ggaaccggag ccgactggat cgttcccaga
     1861 attccggctt ccctcatctc cttatagaat aatgccggct cggtgatttt gtcagaatcg
     1921 tttggagatg cttctttttt ggcaatgatg gcactccgca gggaatctcc gcttacagag
     1981 tgatcaatag gaaaaccggt attctgtcct tcaatcaaca cccatgctcc tttcaatttc
     2041 cccttcatcc gttcaaactc ctgccgggtt ttgggttcaa caaccacatg tccgcgttgt
     2101 actccttttg ttccggatgt atagctaggc gttacaaaat cgagtgacat ggaggtatca
     2161 ctgtacattt tcccaaacca ggggccccgg ttaaaaccca cgggcatttc tcccacttcc
     2221 tgaatttcca cttccaaccc ccactccttc agtttaccgg ccacccagta cgtagcattt
     2281 tcataagcgt cggaacctat cggcctaccg ccgatccggt tggtgagtat atccaaatgt
     2341 tgcatggtgc ggttatccgt tttcccaatc tccatgattt ttttgcttac tttattttgc
     2401 gctccgacag gtagacttcc tatcagcaga acgaggaagc aaacttttct gataatatgc
     2461 atttgaaatg tattttgatt ttttttacaa agatattcat atttgcgtaa aatccgccat
     2521 caaacttcct ttcaaaaagg aaacctcccg aatactttta acttttctta tgcagtgatt
     2581 ttgaaaaatt gggcatctct taagaaacaa atgattaatt ttgttataga tagcaggtaa
     2641 cagatattag actgaaatct gtcagaaagg cggtctgatt ttgatagtgt aacggccaaa
     2701 atatacaata tgaagattcc gcaacaactt ttacttctgt ttttcctgat gtttctggtg
     2761 ttttattcgg tgtgcgcaca agagaacccc atcagaaaag acagtttaag gataatcccg
     2821 aaaaatccgt tgtccttgcc ctccgattct gctgcaccgg gtggtgtttc cgtcattatt
     2881 cccaccatac acccgatcgt gggaaaagac gggcttcaat acaaccgctt caacgacttc
     2941 acggtaaatc cttaccttat gtctcgcaca caaccatcgg gcgttcagtt ttacggtacc
     3001 ggatcggata atattaattc caaatcacga acagccgtag caacctattc accggttccg
     3061 agaataagcc tttattctgc agctacactg ggattagtgg aaaccccttt ttttgggaaa
     3121 ggaaactatt atatactgaa tgccggggct agctatctgc ttacacccaa tctcaatgcg
     3181 gggttatcgg ggatgtataa ttcaaatttt aacgttattc ccttctggag cgtctctgca
     3241 gatttacagt atctggcagg caggaacttg atgttagaag gcagtgccgg ctttatgaag
     3301 accggcgcca acatgtttaa cctgaaccaa tcggcggtgc tgatcgacct tcacgcccgg
     3361 taccgtttgt ctgacgactg gtttctgaat gcctacggcg gatttccggt gacgcaaaag
     3421 accaaccgcc ctggtgtacc catgatgccg atgatggaca acacgcacta cggcggcact
     3481 gttgagtact ggttccaacc cactgtgggt gcagaagcag ggttgatttg ggtacgcgac
     3541 atgttttcgg gcaagatgcg cccccagccg aaactggaac ttaagttcag gccgggaagg
     3601 tagaaaaaaa agctgtctgc taaaaatcag aaacttctga acagaaacca cgcaacaaca
     3661 agcgtcagga caatattaaa tccctgcgca ataagaaacg accagatata ttttgaattt
     3721 tctttcttga agatatccct gaagctggtt tccaggccaa tgcaaacaaa agccaacgag
     3781 aagagcaacc cgcgcgattc ctgggcaatt ttcgagagac ttttcccggt tgccggttca
     3841 aaagcaaagc tgaagatcag cgatgccaca agaaacccaa ggacaaattt tgggaaacgc
     3901 tcccacaaaa cactccccga aggacgaata ttgga
//
LOCUS       DAOSDR010000304         3922 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrimonas sp. isolate AMR_MDS_1826
            NODE_8152_length_3922_cov_4.096199, whole genome shotgun sequence.
ACCESSION   DAOSDR010000304 DAOSDR010000000
VERSION     DAOSDR010000304.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863355
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrimonas sp. (wastewater metagenome)
  ORGANISM  Petrimonas sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Dysgonomonadaceae; Petrimonas.
REFERENCE   1  (bases 1 to 3922)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3922)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:10:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,720
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 35
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3922
                     /organism="Petrimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8152_length_3922_cov_4.096199"
                     /isolate="AMR_MDS_1826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2023866"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..769)
                     /gene="arsM"
                     /locus_tag="PLI30_10870"
     CDS             complement(<1..769)
                     /gene="arsM"
                     /locus_tag="PLI30_10870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009860065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenite methyltransferase"
                     /protein_id="HOI80092.1"
                     /translation="MNAKDLKVVVKEKYGSIAKQSLSNDKIKSSCCGTSGCCEKIEFS
                     MIGDDYNTVEGYNPDADLGLGCGLPTQFANLKTGDCVLDLGSGAGNDCFVARAIVGET
                     GKIVGLDFTDMMVAKAIENNRKLGFMNVEFVQGDIEEMPFSDNDFDVIVSNCVLNLVP
                     NKHKAFAEMIRVLKPGGHFCVSDVVTKGELPETLRSDAEMYVGCVSGAITRDQYINII
                     HDNGFENVAIHKLKPIPIPYKVLSKYMSEEEIKDLKKG"
     gene            complement(799..2118)
                     /locus_tag="PLI30_10875"
     CDS             complement(799..2118)
                     /locus_tag="PLI30_10875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011967355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HOI80093.1"
                     /translation="MEIRKEFKILVTFVVVFLAIYFLPIHSNVFNTAMDATFDLSKWY
                     AREHVVLCLLPAFLIAGVISVFISQGSVIRYFGAKAKKWVAYTVAAVSGTVLAVCSCT
                     ILPLFSSIHKRGAGLGPAIAFLYSGPAINILAIILTARILGWELGAARVIGAVSFSII
                     IGLIMAFIYRKEEKVRADEQMNIIPEPEKRPLWQTSIFFFTLVLILVFANWGKPTSDD
                     TNSFWHFIWENKWYITGVFSLVLATALIFILKMKTWKVVAAVVVTAVSTFIFRNPVIS
                     MVIGIAAVAFLGLTDKADDEENKKWILSSWEFTKQIVPLLAIGVVIAGFLLGSTHDNV
                     AIPGIIPNEWIAWAVGGNSFKSNFLSSVVGAFMYFATLTEVPIIQGLIASGMGKGPAL
                     ALLLSGPSMSLPNLLVIRGIIGTQKTVVYAALVIILSTLSGLIFGSL"
     gene            complement(2122..2835)
                     /locus_tag="PLI30_10880"
     CDS             complement(2122..2835)
                     /locus_tag="PLI30_10880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018462486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic aminobenezylarsenical efflux permease
                     ArsG family transporter"
                     /protein_id="HOI80094.1"
                     /translation="MEQFLQNLLESSNIPFITAFILGLLTAISPCPLATNITAIGYIS
                     KDIEDKKRIFRNGLLYTLGRIVSYTALGIILIFILKQGSSLYGIQKFIAKFGGVIIGP
                     LLIIIGLFMLYGHKLNLPKFGFSGKNSEKIRKKGGWGALWLGVLFALTFCPTSGIFYF
                     GMLIPMSAMEAGGYFFPVVFAIATALPVIIVAWLLAFSFAEVGRFYNSVQKFEKWFSK
                     AIALIFIIIGIYYTYSFYF"
     gene            complement(2843..3292)
                     /locus_tag="PLI30_10885"
     CDS             complement(2843..3292)
                     /locus_tag="PLI30_10885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007218597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrophenyl compound nitroreductase subunit ArsF
                     family protein"
                     /protein_id="HOI80095.1"
                     /translation="MKQLIFICLIGLFTISCNQNKKAEQTTNQSTDEFAPSGDVVEVI
                     YFHGTQRCPTCQAIEAETKSLIESSFADAINSKKLIFKIVDISKPENKAIADRYEVAW
                     SSLFVNDWKKDQESVNNLTEFAFTNARNNPDAFKRGLSEKINEFLAN"
     gene            complement(3297..3530)
                     /locus_tag="PLI30_10890"
     CDS             complement(3297..3530)
                     /locus_tag="PLI30_10890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005839471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HOI80096.1"
                     /translation="MEIKILGTGCPSCKTLYETTKQAVIELGIEANVVKEEDMVKIMQ
                     YNVFSVPALVINEKVVSSGKKLSLEQVKNLLKK"
     gene            complement(3600..3878)
                     /locus_tag="PLI30_10895"
     CDS             complement(3600..3878)
                     /locus_tag="PLI30_10895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015547915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HOI80097.1"
                     /translation="MAAFAKALGHPTRVAIIQFLLSQESCYFGDIHDVFPNAKATVSQ
                     HLKELKNAGLIQGEIETPKVKYCINKENWEIARNLFGNFFASEIKNNC"
BASE COUNT         1136 a          869 c          736 g         1181 t
ORIGIN      
        1 ctcctttttt taaatcttta atttcttcct cagacatata tttcgaaaga actttatagg
       61 gaattggtat tggcttcaac ttgtgaattg ctacgttctc aaaaccgttg tcatggatga
      121 tattgatata ttgatcccgc gtaatggcac ccgaaacaca tcctacatac atttctgcat
      181 cagagcgcag ggtttcaggc aactcacctt tggtcactac atcggaaaca caaaaatgtc
      241 ctccgggttt cagtacgcga atcatttcag caaatgcttt gtgcttattg ggcaccagat
      301 ttagaacgca gttcgataca atcacatcaa aatcgttgtc agaaaaaggc atttcttcga
      361 tgtcgccttg cacaaattcc acattcatga atccgagttt ccgattgttt tcaatagctt
      421 tggcaaccat catgtccgtg aaatccagac ccacgatttt gcctgtttct cccacaatgg
      481 cgcgggctac aaagcaatcg tttccggcac cgcttcccaa gtcgagcaca cagtcgccag
      541 ttttcagatt ggcaaactga gtagggagtc cgcaccccaa gcccaaatct gcatcgggat
      601 tatagccttc tacggtgttg tagtcatcgc caatcatgct gaattcaatt ttctcacaac
      661 aacccgaagt tccgcaacag gatgatttaa tcttatcatt tgacaagctt tgtttggcta
      721 ttgaaccata tttttccttt acaaccactt ttaaatcttt agcattcata tatttaattt
      781 gattttagct ttgcttaatt ataaacttcc aaaaattaat ccggataggg ttgacaaaat
      841 aatgactaac gcagcataga caactgtttt ctgtgttcca ataattccac ggataaccaa
      901 taaatttggc aaagacatcg atggccctga aagtaacagt gccaatgccg gacctttccc
      961 cattcccgag gcgattaatc cctgaataat tggaacttct gtaagtgtgg caaaatacat
     1021 aaacgcaccc acaacggagg aaagaaaatt cgatttgaat gagtttccgc ccaccgccca
     1081 ggcaatccat tcgttcggaa ttattccagg aatagccaca ttatcgtgcg tggaacccag
     1141 caaaaatccg gcaatgacca cgcctattgc caataaggga acaatttgtt tcgtaaactc
     1201 ccacgatgaa agaatccact tcttgttttc ttcgtcgtcc gctttgtccg ttaatccaag
     1261 gaatgcgacg gcagcaattc ctattaccat tgaaatgact ggattcctga aaataaatgt
     1321 acttacggct gtaaccacta cggcagcaac tactttccat gttttcatct tcagaatgaa
     1381 aatcagggct gtagccagta ccagactaaa aacaccggta atgtaccact tgttttccca
     1441 tataaaatgc caaaagctgt ttgtgtcgtc tgatgtgggt tttccccaat tggcaaaaac
     1501 caaaatcagt accagtgtga aaaagaaaat agatgtttgc cacaacggtc gtttttcggg
     1561 ttcaggaata atgttcatct gttcatcggc ccgtactttt tcctcttttc ggtaaatgaa
     1621 cgccataatc aaaccgatga taatgctgaa cgaaacggct ccaataacgc gtgctgctcc
     1681 caattcccaa cctaaaattc gggcggtaag aattatagca agaatgttaa tggcaggacc
     1741 tgaatataaa aaagcgatag cgggtcctaa tccggcacca cgtttatgaa tactcgaaaa
     1801 caagggtaaa atcgtgcagg aacaaaccgc cagaacagtt cccgaaacag cagccaccgt
     1861 ataggcaacc catttttttg cttttgctcc aaaatatctg ataacggagc cttgactgat
     1921 aaaaaccgaa ataactcccg ctattaaaaa agcgggtaac aaacaaagta ccacgtgctc
     1981 ccgggcgtac catttcgata aatcgaaagt ggcatccatg gccgtgttga acacgttgct
     2041 gtgaataggc aagaaatata tcgccaagaa cacaacaaca aaagtgacaa gtatcttaaa
     2101 ttcttttctg atttccattt tctaaaaata aaagctgtac gtgtaataaa ttcctataat
     2161 gataaagatt aaggcaatgg ctttactaaa ccatttttcg aatttctgaa cgctgttgta
     2221 aaaccgccca acttcggcaa aactgaaagc cagtagccac gccacaataa ttacaggcaa
     2281 tgctgttgca atagcaaata caactggaaa aaaatatcct cctgcttcca ttgccgacat
     2341 cggtataagc attccgaagt agaatattcc gcttgtcgga caaaatgtca aggcgaaaag
     2401 tacacccagc caaagtgcac cccaaccacc tttttttctg attttttcag agtttttccc
     2461 actgaacccg aatttgggca aattcagctt gtgtccgtac aacataaaca gcccgataat
     2521 aattaatagt gggccgataa tgacaccgcc gaatttcgca atgaactttt gaattccgta
     2581 aaggcttgaa ccttgtttga ggatgaaaat cagaatgatg cctaatgccg tgtatgaaac
     2641 tattctgccc aatgtgtata gtaacccgtt tctgaaaatt ctttttttat cttcaatgtc
     2701 tttacttatg tagccaatag cggtaatgtt ggtagctagc ggacaaggac tgattgcggt
     2761 aagcaacccg agaatgaatg ccgtaataaa cggaatgtta ctgctttcga gtaagttttg
     2821 aagaaactgc tccatataag gattaattgg ccaaaaattc gtttattttt tcgcttaatc
     2881 cccttttgaa ggcatcggga ttgttgcggg cattggtaaa agcaaactct gtgagattgt
     2941 ttactgattc ctgatctttt ttccagtcgt tgacaaaaag cgatgaccac gccacttcgt
     3001 acctgtcggc gatggcctta ttttccggct tggagatatc cactattttg aatatcaatt
     3061 ttttgctgtt aatggcgtca gcgaaactgc tttcgatcag acttttggtt tctgcttcga
     3121 tggcctgaca ggttggacaa cgctgtgtgc cgtgaaaata aattacctcc accacgtcgc
     3181 ccgatggagc aaattcatct gtggattgat tggtggtttg ttccgctttt ttattttgat
     3241 tacaacttat cgtgaatagt ccgataaggc agataaagat taactgtttc attgcgctac
     3301 tttttcaata gattttttac ttgttcgagt gataattttt ttcctgacga taccactttt
     3361 tcgttgataa ccaatgccgg aacggagaac acattgtact gcataatttt taccatgtcc
     3421 tcttctttta ccacattagc ctcaatacca agttcaatta ccgcttgttt tgtagtttcg
     3481 tacagtgttt tgcaacttgg acatcctgtt cctaaaattt taatttccat aatttctgta
     3541 ttttattgat tttattgttt atagttcgtc aaacaacgaa cattgtttgt aaaaaatttt
     3601 tagcaattat ttttgatttc cgatgcgaaa aagtttccaa acaggttgcg ggcaatttcc
     3661 cagttctctt tgttgatgca atacttcact ttgggagttt caatttcacc ctgaataagc
     3721 ccggcgtttt tcaactcttt caggtgttgc gatacggttg ccttggcatt gggaaacaca
     3781 tcgtggatat cgccgaaata acacgactcc tgcgacagca aaaactggat gatcgccacc
     3841 cgggtaggat gtcccagcgc tttagcgaat gctgcaattt gtttgtctgt tgcttgttgg
     3901 gcactctctt ttttcttaac ca
//
LOCUS       DAOSDR010000305         3919 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrimonas sp. isolate AMR_MDS_1826
            NODE_8164_length_3919_cov_3.631470, whole genome shotgun sequence.
ACCESSION   DAOSDR010000305 DAOSDR010000000
VERSION     DAOSDR010000305.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863355
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrimonas sp. (wastewater metagenome)
  ORGANISM  Petrimonas sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Dysgonomonadaceae; Petrimonas.
REFERENCE   1  (bases 1 to 3919)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3919)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:10:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,720
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 35
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3919
                     /organism="Petrimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8164_length_3919_cov_3.631470"
                     /isolate="AMR_MDS_1826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2023866"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..827)
                     /locus_tag="PLI30_10900"
     CDS             complement(<1..827)
                     /locus_tag="PLI30_10900"
                     /inference="COORDINATES: protein motif:HMM:NF018072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HOI80098.1"
                     /translation="MKIGKLFLAGIVALGLGLTGCNNDDVPELSGEKEATISIKVLPS
                     SNGSLLRSTGNLNGDGVLAKGLAQESAIKSLEVWIFKGDVLDAYKSAAAAEVTEIEAR
                     IGARTVVVVANAEIGQKNSKAELLATIKGLPADVAGKGLVMTAEPFDVTLVKGNNYYG
                     YTDAEVAAKTGENKNHLNDNKKPLPITRVNARVAIVSAVLDLASVPANQKVVFDALKD
                     VEVAMFNVPKETKLFGSDLAKNENYLFGAKWTSPLSTYVGSADASAVATASLTDAVTG
                     "
     gene            complement(930..2396)
                     /locus_tag="PLI30_10905"
     CDS             complement(930..2396)
                     /locus_tag="PLI30_10905"
                     /inference="COORDINATES: protein motif:HMM:NF024386.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3868 domain-containing protein"
                     /protein_id="HOI80099.1"
                     /translation="MKRKSIYRLLVTMTALFVSHEVVTAQVNYTDQIVIENQAISKKG
                     GVTSVTIDMNLDNLRLNKNDMLIITPVIVSNQNEVELESIAVKGTLRNKVLQRPFEWN
                     GKTHLSMPVEKQLVRRNGTAQSMHYTATLPYSDWQRNARLLLRTEVVGCADCSDMQPD
                     KLVSQRILADKFIPDFRMSYIVPEVEEVKVRSEIFSAHLNYIVGRYDLLPDFQNNAEQ
                     LARVDSVVHALKADSDLSITNFTISGYASPEDTYERNMLLSQRRAETFARYMEKKYGY
                     ARDRFNVQWFGEDWEGLRKAVEGSSLTDKEAVLNIIDNVGINEGREKRLMELNGGSTY
                     RLMLREYFPPLRRNDYEVTFVSRTFNVEEAKELIKTKPKVLSLNEMYLVANTYPADSP
                     QYREVFDIACRTFPDAEVACLNAAVGELRANRPDAALAYLEQYNESPAAMNLMGVAYA
                     QKRDTARAKQYFNRAIQAGNADAEYNAKQLQQYIEDNL"
     gene            complement(2413..2991)
                     /locus_tag="PLI30_10910"
     CDS             complement(2413..2991)
                     /locus_tag="PLI30_10910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004584647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3575 domain-containing protein"
                     /protein_id="HOI80100.1"
                     /translation="MLKHNKLILIIVLILGTYQLHAQTVGVKTNFAHWAAAGTPNVGL
                     EFALNRKYTLEVGGGFNPFVFNDNKKAKHWIVQPELRYWLCESFNGHFLGLHALAGEY
                     NIGGIDIPVGRLSNLNEFRYEGFAYGAGISYGHQWLISPRWNFELSIGAGYAYLTYDK
                     FTCVKCGSKISSDTQNYLGVTKAAISLIYLIK"
BASE COUNT         1047 a          842 c          762 g         1268 t
ORIGIN      
        1 ccggttacag cgtcagtcaa agaggctgtg gcgactgcag aagcatcggc agatcccaca
       61 taagtgctta gcggtgaagt ccattttgca ccaaacagat agttctcgtt cttagccaaa
      121 tcgctgccaa acagttttgt ttctttcggg acgttaaaca tggccacttc cacatctttc
      181 aaagcatcga ataccacttt ctggttagct ggtacacttg ccagatccaa cacagcacta
      241 acaattgcta cgcgggcatt gacacgggtg attggaagag gttttttgtt atcgttcaaa
      301 tggtttttat tttcgccggt tttagcagct acttctgcat ccgtataacc gtaatagtta
      361 ttgcctttta caagagttac atcaaacggt tctgccgtca tgaccaatcc ttttccagca
      421 acatcagccg gcaacccctt gattgtcgca agaagttctg ctttcgagtt cttttgtcca
      481 atctctgcat tggccacaac caccactgtg cgggctccga tgcgggcttc aatttcagtt
      541 acttcagctg cggcagcact cttataggcg tctaatacat cgcctttgaa gatccatacc
      601 tccaaggatt tgatcgcgct ctcttgagcc aatccttttg ccagaacacc gtcaccgttc
      661 aaattgccgg tagaacgtag taaagagccg ttgctggaag gtaatacctt gatcgagatg
      721 gtagcttcct tttcaccgga cagttccggt acgtcgtcgt tgttacatcc tgtaagccct
      781 aatcccaagg ctacaattcc tgctaaaaat aattttccga ttttcataag ttgataattt
      841 aaaaagtgaa taatttagtt taatttgatc tgtttaatac gtttgtttac ttttaatgtt
      901 tattcttaag actctcatct tcaagctctc tacaagttat cttctatata ttgttggagt
      961 tgttttgcgt tgtactctgc atcggcattg cctgcctgaa tagctctgtt gaagtattgt
     1021 ttggctcggg cagtatctct tttttgggca tatgctaccc ccattagatt cattgctgca
     1081 gggctctcgt tatactgctc cagataggcc aaggctgcgt cgggcctgtt tgcacgtaac
     1141 tcacctacag cagcgtttaa gcaggcaact tcggcatcgg gaaaagttcg acaggcaata
     1201 tcaaatactt ccctgtattg cgggctatct gctggatagg tattggccac cagatacatc
     1261 tcattgagac ttaatacttt aggttttgtt ttaataagtt ctttggcttc ctcaacgtta
     1321 aacgtacgtg aaacgaacgt cacctcataa tcgttgcggc gaagcggtgg aaaatactcc
     1381 ctgagcatga gcctgtaggt tgaaccgccg ttcaactcca tgagtcgttt ttcgcggccc
     1441 tcgtttatac ctacgttgtc gataatgtta aggacagctt ctttatcggt caaagaggaa
     1501 ccttcaacag ctttccgtaa tccttcccag tcttcgccga accactgtac gttaaaccgg
     1561 tcccgtgcat agccgtattt cttctccata taacgggcaa acgtttcagc acggcgttga
     1621 gagaggagca tattgcgctc gtacgtgtct tcaggggaag catagccgga gatagtgaaa
     1681 ttagtaattg agagatcaga atcggctttc agtgcatgaa ctacactgtc tactctggcc
     1741 agttgctcag cattgttctg gaagtccggc agtaaatcgt aacgtcccac gatgtagttg
     1801 aggtgtgcgc tgaatatttc gcttcgcact tttacttctt ccacttcggg tacgatatag
     1861 gacatgcgga aatcgggaat gaacttgtcg gccaggatcc tctgcgaaac caatttatcg
     1921 ggttgcatgt ccgagcaatc ggcacaaccc actacttccg ttctcagtag caaccgtgca
     1981 tttcgttgcc agtccgaata tggcagggtg gcggtataat gcatcgactg ggccgtaccg
     2041 ttccggcgca ccaattgttt ttccaccggc atggaaaggt gtgttttccc attccactca
     2101 aacggccttt ggagcacttt attccgtagt gtccctttca cggctataga ttctaattct
     2161 acttcatttt gattggagac tattacaggt gtgataatca gcatatcatt tttgttgagc
     2221 ctcaagttgt cgaggttcat atctatggtc acgcttgtta cgcccccttt cttagatata
     2281 gcctggtttt caataactat ctgatccgtg tagttgactt gagccgttac tacctcatgg
     2341 cttacgaaca aggcagtcat cgtaacaagc aatctgtata tacttttccg tttcatacgt
     2401 acttccattt ttttatttta taagataaat aagggatatt gccgctttag ttacacccaa
     2461 atagttttgg gtatcgctgg atattttgct tccgcacttc acacaggtaa acttgtcgta
     2521 cgtcaggtaa gcatagcctg cgccgatgct gagctcaaaa ttccacctgg gtgagattaa
     2581 ccactgatgc ccgtaactga tgccagctcc gtaagcaaaa ccttcgtagc ggaattcgtt
     2641 taaattggat aaacgaccta ctggaatatc tattccgccg atgttgtatt cgcctgccaa
     2701 tgcatgcaat cccaaaaaat ggccgttgaa actttcgcaa agccaatacc tcagctcggg
     2761 ctgaacaatc cagtgttttg cttttttgtt gtcgttaaat acaaaagggt tgaatcctcc
     2821 tcctacttcg agtgtgtact tacgattaag ggcaaattct aaaccaacat ttggtgtacc
     2881 tgctgccgcc caatgagcga aattggtttt gactccgact gtctgggcat gtaattgata
     2941 cgttcccagt atcaatacta taatcagaat taatttatta tgcttgagca ttttatttgt
     3001 aatattgggt ttgtaaatgt gtgaaactgc aaaaaacaga cacaaagttg tatttttcgg
     3061 actcataaat gactgataaa tctcctattt tataaaatag tgtatctttt ccacattttt
     3121 ttctcataaa tgaacccttt tagtcacctt attttttacc tttatgaata aaggcgggaa
     3181 aggtctaaat aatttttaat agggtttttt attgagaatt catttttttg ttgatgcaaa
     3241 attaggatag cccaaaagaa aaagttataa ctaaaacgac ttttttttgc acttttgaga
     3301 cacttcagga agttaaattt ggactaaacg agcatgatga aaaaatcata cttctgataa
     3361 tgacaaggtt aaaaagtaac tttttataca aatccgataa cgaaaaaaag tcaaaatatt
     3421 taataaaaag gacacattcg agaaaatcag cgaaaataac cgttaaaaca caactgttta
     3481 tatattagat tcttgatgtt ttttcttcat aattgacagc tttttttgaa gacaatcttt
     3541 tttcctattt cataaaatat tcccaaatcg tcaaaaaaat tctcaaacgg taaaaaataa
     3601 aagaataaaa ggagatgaaa tttgcagcag atagattctt tttttaattt tgtcctaaat
     3661 ttgttgccat gttataccct attttttcta tcctgcccgc agttgtatgt gtgttttgga
     3721 tatttttact gctcgttgat aagcaaaaaa acaggtcgaa aaagtttttt atcctactgc
     3781 tgatctttat actggtcaac tttattgctc atgccgcttt tttcaatcat aaatatgaat
     3841 tatataccgt gctagacagc atatgggtgt ttacttcatt gcttattttt ccactgtact
     3901 actattactt aaggatatt
//
LOCUS       DAOSDR010000306         3914 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrimonas sp. isolate AMR_MDS_1826
            NODE_8179_length_3914_cov_3.168956, whole genome shotgun sequence.
ACCESSION   DAOSDR010000306 DAOSDR010000000
VERSION     DAOSDR010000306.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863355
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrimonas sp. (wastewater metagenome)
  ORGANISM  Petrimonas sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Dysgonomonadaceae; Petrimonas.
REFERENCE   1  (bases 1 to 3914)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3914)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:10:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,720
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 35
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3914
                     /organism="Petrimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8179_length_3914_cov_3.168956"
                     /isolate="AMR_MDS_1826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2023866"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..241
                     /locus_tag="PLI30_10915"
     CDS             <1..241
                     /locus_tag="PLI30_10915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019538351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HOI80101.1"
                     /translation="DLLNCKYDKSQVGLFVWGKLPESVMSSKDFIDDILYNARVFITP
                     GVIFGSNGEGYIRISLGASVEKMEEAYNRILTYNT"
     gene            253..1323
                     /locus_tag="PLI30_10920"
     CDS             253..1323
                     /locus_tag="PLI30_10920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018108343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-deoxy-7-phosphoheptulonate
                     synthase/chorismate mutase type II"
                     /protein_id="HOI80102.1"
                     /translation="MEFESILLPGVEDKRPIIISGPCSAETEEQVMETAKKLAEKGVK
                     IFRAGVWKPRTKPGGFEGVGTPALKWLQQVRKETGMYVATEVATEKHVYESLKYNMDM
                     LWIGARTTANPFAVQEIADTLRGVDIPVLIKNPVNPDLELWIGAIERLYNAGIKKIGV
                     IHRGFSTNDKTVYRNIPQWHIPIELKRRYPDLPVICDPSHIGGKRSMIKTISQQAMDL
                     NYYGLIIESHRNPDDAWSDSSQQISPETLAEILNELVIRDKVQSTEDLSDLRRQIDDL
                     DNEILQLLSKRMRVSREIGLYKLEHDMPVLQTGRYDHIRKDRVEQAEKMEMAGEFALK
                     ILEAIHTESVRQQFEVMERAKK"
     gene            1320..2093
                     /locus_tag="PLI30_10925"
     CDS             1320..2093
                     /locus_tag="PLI30_10925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010536552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004665 - prephenate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0008977 - prephenate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0006571 - tyrosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase/arogenate dehydrogenase
                     family protein"
                     /protein_id="HOI80103.1"
                     /translation="MKILILGAGKMGSFFADVLSFDHEVAVLDTDPQKLRFIYNTLRM
                     TKPEEVRDFAPELVINAATLKYTVEAFHSVLPYLPQTCILSDIASVKTGLEEFYRERT
                     RPYVSTHPMFGPTFASLSDLSTQSAIIISESSHLGKVFFRDLYRRLKLNVFEYTFREH
                     DETIAYSLSIPFASTLVFASVMKHQDAPGTTFKKHMNIAQGLLSEDDFLLTEILFNPY
                     TPDQLVKIREKLKELLAIIEVRDSEAMKVFLTQVRKNIE"
     gene            complement(2099..2911)
                     /locus_tag="PLI30_10930"
     CDS             complement(2099..2911)
                     /locus_tag="PLI30_10930"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007659164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0016018 - cyclosporin A binding [Evidence
                     IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HOI80104.1"
                     /translation="MKKYILSTVLFAFSLLANAQTEYPVIIIETNYGTMKAVLYDDTP
                     NHSNHFLKLVKQGYFNGTLFHRVVREFMIQGGAQDSKNAPAGAQIGGGRTDMDIMPEF
                     RDNHFHKKGALAAPRRGDNENPQKKSDASQFYIVHGKVYSPGRLDSMEMAVNVPIKNK
                     IIRDHYLPHKDDLAKLKATDASAFNAMLDSVLHVVDSLYEAAPGKFFLPEELKETYST
                     IGGAHHLDGEYTVFGEVTEGLEVIDKIAALPIDSNSRPRSDAKIVRIYVESK"
     gene            complement(2973..>3914)
                     /locus_tag="PLI30_10935"
     CDS             complement(2973..>3914)
                     /locus_tag="PLI30_10935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019538355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LptF/LptG family permease"
                     /protein_id="HOI80105.1"
                     /translation="LKEIIFNYYINFVPYYANLFSPLFIFLAVIFFTSKMASSSEIIA
                     LLSNGVSFKRLLKPYMVSAAIIAVFSFVMGSFIIPPANKTRIDFEQKYIDPRKRKTGD
                     RDIQFKVAEGTIVYFGNFDMESKTGYNFSIDHFDSLKLVSRLTAQSIKYDSLHHWTIS
                     NYQIRDFDGLEEKITHGTTTDTIIQIVPNDFIVAKTDQQMMTTPQLLRHVRSQKRRGL
                     GNIQAFQIEYHSRYASIFSAFILTIIGASLSARKVKGGMGLNIGIGLALSMAYILFMT
                     ISSSFAVKGSMSPFVAAWIPNIVFIGIAFFLYKKAPN"
BASE COUNT         1072 a          895 c          953 g          994 t
ORIGIN      
        1 ggatctgctg aactgcaagt acgataaaag ccaggttggc ctttttgttt ggggaaaatt
       61 gccggaatct gtgatgtcga gcaaagactt tatcgatgat atcctgtaca acgcccgcgt
      121 ttttattacc cccggtgtaa tcttcggaag caatggcgaa gggtatatcc ggatttctct
      181 cggcgcatcc gttgaaaaaa tggaggaagc ctacaatcga attttaactt acaacacatg
      241 acaaagaaaa atatggaatt tgaatcaatt ttattacccg gggtcgagga caagcgcccg
      301 atcattattt ccggcccatg tagcgcggaa acggaagagc aggtcatgga gacggcaaaa
      361 aaacttgctg aaaaaggggt gaagatcttc agggcgggtg tgtggaagcc acgcacaaag
      421 cccggtggat ttgaaggggt cggcaccccg gcattaaaat ggctccagca ggtacgcaaa
      481 gagaccggta tgtatgtggc gaccgaggtg gccacggaaa aacatgtgta tgaatctctg
      541 aaatacaaca tggatatgtt gtggatcgga gcccgcacca cagcaaatcc ttttgccgta
      601 caggaaatag cagatacatt aaggggtgtg gacatcccgg ttctgataaa aaatccggtg
      661 aatcccgacc tggagctatg gatcggtgcc attgaacgtt tatacaacgc aggcattaag
      721 aagatcgggg tcattcaccg cgggttcagt accaacgaca agaccgttta tcgcaacatt
      781 cctcaatggc acatccccat cgaattgaaa cgccgttacc ctgaccttcc cgtcatttgt
      841 gatccaagcc atattggtgg aaaacgctcc atgattaaaa cgatttccca acaggccatg
      901 gacctgaatt actacgggtt gatcatcgag tctcaccgta accccgatga tgcctggagc
      961 gacagttccc agcaaatctc tcccgaaacg ctggctgaaa ttctgaatga actggttatt
     1021 cgcgacaagg tgcaatcgac ggaggactta tccgacctgc gccggcaaat agatgacctg
     1081 gacaatgaaa tcctgcaact cttatcgaaa cgtatgcgcg tatcccgcga aatagggctc
     1141 tacaagctgg agcacgatat gcccgtactc cagaccggac gctacgatca tatccgcaag
     1201 gaccgcgtgg agcaagccga gaaaatggag atggcgggtg aatttgcctt gaaaatcttg
     1261 gaagccattc acacggaatc cgttcgtcag cagtttgaag taatggaaag agcaaaaaaa
     1321 tgaaaatact catcttagga gctggcaaaa tgggatcgtt ctttgccgat gtgctcagtt
     1381 tcgatcacga agtagccgta ttggataccg atccccaaaa actacggttc atatacaaca
     1441 cactccggat gacaaagccg gaggaggtgc gggattttgc ccccgaacta gtgatcaatg
     1501 ccgctacgct gaaatacacc gtcgaggcgt ttcacagtgt gctgccttat ttgccgcaaa
     1561 cgtgtatcct gagtgatatt gcatcggtaa aaaccggatt ggaagaattt taccgggaac
     1621 gtacccgccc gtatgtttcc actcacccga tgttcgggcc tacgtttgcc agcctgagcg
     1681 acctcagcac gcaaagtgcc attatcatat cggagtcgtc gcacctggga aaggtttttt
     1741 ttcgcgatct gtaccgccgg ctcaagctaa acgtgtttga atacactttc cgggagcacg
     1801 acgaaaccat cgcttactcg ctttccatcc cgtttgcatc cacattggtg tttgcatcgg
     1861 taatgaagca tcaggatgcg ccgggaacca cgttcaaaaa gcatatgaac atagcacagg
     1921 ggctgctttc ggaagatgat tttctcctta cggagatctt gttcaaccct tatactcccg
     1981 atcaattggt gaaaatcagg gagaagctga aagaactatt ggctattatc gaagttaggg
     2041 acagcgaagc catgaaggta tttttgacgc aggtaagaaa gaatattgag tagggtggct
     2101 atttagactc cacataaatc ctcacaatct tcgcatctga tctagggcgg ctgttgctgt
     2161 cgataggcag ggcggcaatc ttgtcaataa cctccagccc ttcggtgact tcgccaaaaa
     2221 cggtgtattc accgtccaga tgatgtgctc cgccaatcgt ggaataggtt tctttgagtt
     2281 cttcaggaag aaaaaacttg ccgggagcgg cttcatacaa cgaatccacc acgtggagga
     2341 ccgaatccag catagcgtta aaggcactgg catcagtggc ttttaattta gccaggtcgt
     2401 ctttatgcgg taagtaatgg tcgcggataa tcttgttttt gatgggcacg tttaccgcca
     2461 tttccatgga atccagccgt cccggtgaat ataccttccc gtgcacgata tagaactggg
     2521 aggcgtctga ttttttctgc gggttttcgt tatctcccct tctcggagca gccagagctc
     2581 cttttttatg aaaatgattg tccctgaact cgggcataat atccatatcg gtacgtccgc
     2641 cgccgatctg tgctccggca ggagcattct tcgaatcctg tgctcctccc tgaatcataa
     2701 attccctaac tacccggtga aacaacgttc cgttaaagta tccctgtttt actaatttca
     2761 ggaaatggtt gctgtggttg ggggtgtcgt cgtacaaaac ggctttcatt gtaccgtaat
     2821 tggtttcgat aatgataacc gggtattccg tttgagcatt ggcgagcaat gaaaatgcga
     2881 acagaacggt ggaaaggata tattttttca tgtctcaact gattttttga attacagatc
     2941 gcaggcatgc ctgctgcaac ggattgtaaa tcctaatttg gcgccttctt gtaaaggaag
     3001 aacgcgatgc cgataaagac aatattgggt atccatgccg ccacaaaagg actcatgctc
     3061 ccttttacgg cgaaagatga actgatggtc atgaacagaa tataagccat actcaaagcc
     3121 agtccgattc caatgttcag ccccatgccc cccttcacct tccgtgctga gagggaagct
     3181 ccgataatgg tcaagataaa agcagaaaat atagaagcgt agcgcgaatg gtattcaatc
     3241 tgaaacgctt gaatattacc taatcccctt ctcttctggc ttctgacgtg gcggagcaat
     3301 tgcggggtag tcatcatctg ctggtctgtt ttggccacga taaaatcgtt ggggacgatt
     3361 tgtatgatgg tatcagtggt ggttccgtgg gtgattttct cttccagccc gtcgaaatcc
     3421 cttatctggt aattgctaat tgtccagtga tgcaacgaat cgtacttaat ggattgggcg
     3481 gtcagccggg agaccagctt caggctgtcg aaatgatcaa tggaaaaatt atagcccgtt
     3541 ttagactcca tatcgaaatt accgaaatag acaatcgtcc cttcggctac cttaaactgt
     3601 atatccctgt cacccgtttt ccgtttacgc gggtcgatgt atttttgttc aaaatcaata
     3661 cgcgttttgt tggcgggggg gataataaag ctgcccatca cgaaggaaaa aacagcaata
     3721 atggctgctg aaaccatgta gggtttgagc aaccgtttaa aactgactcc gttggataaa
     3781 agagcaatta tctccgaact ggaagccatt ttggaggtga aaaagattac tgccagaaag
     3841 ataaaaagcg gactgaaaag attggcgtag tacggaacga aattgatgta ataattaaaa
     3901 attatttctt tcag
//
LOCUS       DAOSDR010000307         3911 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrimonas sp. isolate AMR_MDS_1826
            NODE_8185_length_3911_cov_3.620851, whole genome shotgun sequence.
ACCESSION   DAOSDR010000307 DAOSDR010000000
VERSION     DAOSDR010000307.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863355
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrimonas sp. (wastewater metagenome)
  ORGANISM  Petrimonas sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Dysgonomonadaceae; Petrimonas.
REFERENCE   1  (bases 1 to 3911)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3911)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:10:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,720
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 35
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3911
                     /organism="Petrimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8185_length_3911_cov_3.620851"
                     /isolate="AMR_MDS_1826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2023866"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..408)
                     /locus_tag="PLI30_10940"
     CDS             complement(<1..408)
                     /locus_tag="PLI30_10940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit I"
                     /protein_id="HOI80106.1"
                     /translation="MKKFTFLAYYRDYDAFLHDLRDLGLIHVAASDRQVEDNEQLDEF
                     LVKLKQLREAQKILNRQIDKKSAAPLNEPDIERGKTIPREIELIQNATASLNQQLQVS
                     IKERELLRPWGNFDPENIERLRKAGYLLDFFIVP"
     gene            complement(414..1028)
                     /locus_tag="PLI30_10945"
     CDS             complement(414..1028)
                     /locus_tag="PLI30_10945"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019537731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HOI80107.1"
                     /translation="MAIKFQYNKTSRQQLEKQLKIRERALPTLQNKETALRMEVKRAK
                     NEIDELEKELEKQIRSYQKMVGMWLEFDPSLLRVKDVHFSKKKIAGVLIPILETVDFE
                     TRPFSVFNRPKWYLDGVRTLETLAEIGIRRDFHELRIQRLDYARKKTTQKVNLFEKVQ
                     IPGYQDAILKIKRYLEDEENLSKSSQKIMRAHLEKKKEEEDSAW"
     gene            complement(1041..2360)
                     /locus_tag="PLI30_10950"
     CDS             complement(1041..2360)
                     /locus_tag="PLI30_10950"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002562682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HOI80108.1"
                     /translation="MMAKAFQKIYTKITQITKATCSVKATDVGYDELATVDGRLAQVV
                     KIINDEVTLQIFEGTEGIPTNAEVVFLGKAPSLKVGDQLAGRFFNAYGDPIDGGPVPE
                     GEERQIGGPSVNPVRRKQPSELIATGISGIDLNNTLVSGQKIPFFADPDQPFNQVMAT
                     VALRAKADKIILGGMGMTNDDYLYYKNVFSNAGALDRIISFMNTTENPAVERLLVPDM
                     ALTAAEYFAVDKNEQVLVLLSDMTSYADALSIVSNRMDQIPSKDSMPGSLYSDLAKVY
                     EKAAQFPDGGSITIIAVTTLSGGDITHAVPDNTGYITEGQLFLRRDSDVGKVIVDPFR
                     SLSRLKQLVQGKKTREDHPQVMNAAVRLYADAANAKTKLENGFDLTDYDQRALSFARD
                     YSEYLLAIDVNLDTVEMLDTTWTLLSKHFKPQEVNMKREMVDRYWKQ"
     gene            complement(2364..>3911)
                     /locus_tag="PLI30_10955"
     CDS             complement(2364..>3911)
                     /locus_tag="PLI30_10955"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019537729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HOI80109.1"
                     /translation="VEFRGHMLEVVLGPGLLERNLDGLENDLDKMEGVFLKRGQYTFP
                     LDEEKKWAFKPIAQVGDNVSGGSWLGEVDENFQPHKIMVPFVMTSEYKVKSIAPEGEY
                     NIYHTVAVVEDKNGEEHKLNMIQKWPVKVPVTLYKEKPRPFKLLETGVRTIDTLNPIV
                     EGGTGFIPGAFGTGKTVLQHAISKQAEADIVIIAACGERANEVVEIFTEFPELDDPHT
                     GRKLMERTIIVANTSNMPVAAREASVYTAMTIGEYYRSMGLKVLLMADSTSRWAQALR
                     EMSNRLEELPGPDAFPMDLSAIISNFYGRAGHVTLNNGKEGSITFIGTVSPAGGNLKE
                     PVTENTKKVARCYYALEQDRADRKRYPAINPIESYSKYIEYPEFEEYISKEISGDWTT
                     KVNDIKTRLLRGKEIAEQINILGDDGVPVEYHITFWKSELIDFVILQQDAFDEIDSVT
                     PIERQKYILDMVVDICRAEFEFENFNQVAEYFKKLINVCRQMNYSEFKSDKFNDYHRQ
                     LEELINN"
BASE COUNT          849 a          998 c          906 g         1158 t
ORIGIN      
        1 ggggactatg aagaaatcca gtaagtatcc tgcttttcgt agccgttcaa tgttttcagg
       61 atcgaagttt ccccagggtc gcagtaattc gcgttctttg atgctcacct gcaattgctg
      121 gttcaacgaa gccgttgcgt tctggataag ttcaatctcg cggggaattg tttttccccg
      181 ttcgatatcg ggttcattta gcggggccgc tgatttttta tcgatttgcc tgttcaatat
      241 tttttgtgcc tcccgcaact gttttaactt caccaggaac tcgtccagtt gttcgttatc
      301 ttccacctgc cggtccgacg cagccacatg aataagcccc agatcgcgca aatcatgcaa
      361 aaacgcatcg taatccctgt aataggcaag gaaagtgaat tttttcattt ttgctaccat
      421 gcgctatcct cctcttcctt ctttttctct aaatgagcac gcataatctt ttgcgatgac
      481 ttggagagat tttcctcgtc ctcgaggtat cgttttattt tcaggatggc atcctgataa
      541 ccgggtatct gaactttctc gaacagattt actttctgag tcgtcttctt tctcgcgtaa
      601 tcgagccgct gaatccgtaa ctcgtgaaaa tctctccgta tacctatttc ggcaagcgtt
      661 tcgagtgtcc ggaccccgtc caggtaccat ttcggacgat taaaaacact gaacggccgg
      721 gtttcgaaat ccaccgtttc cagtatcggg atcaatacgc cggctatttt cttttttgag
      781 aaatgaacat cttttacgcg gagaaggctc gggtcgaatt cgagccacat tcccaccatt
      841 ttttggtaag accggatctg tttttcgagt tctttttcca gctcatcgat ttcatttttt
      901 gcccttttta cctccatacg caaggcagtt tccttgtttt gcaacgtagg aagtgcacgc
      961 tcgcgtatct tcagttgttt ttccaactgc tgacgcgacg ttttgttgta ttgaaatttt
     1021 atagccataa actttacctg ttactgcttc cagtacctgt ctaccatttc gcgcttcatg
     1081 tttacttcct gcggtttgaa atgcttcgat aacaacgtcc aggttgtatc cagcatctca
     1141 acggtgtcga ggtttacgtc tatggcgaga aggtattcgg aatagtccct ggcaaacgac
     1201 aatgcccgct ggtcataatc ggtcaggtcg aagccgttct cgagtttggt tttcgcattg
     1261 gcggcatcag cgtacaagcg tacggcagcg ttcatcacct gcgggtggtc ttcacgtgtc
     1321 ttcttgcctt gaaccagttg tttcaatcgc gaaagcgaac ggaaaggatc cacgattacc
     1381 ttacctacat cgctgtcccg gcgaaggaac agctgtcctt cggtaatgta tccggtatta
     1441 tcgggaacgg cgtgtgtgat atcgccgccc gaaagcgttg taacagcgat gatcgtgatg
     1501 gaaccgccat cggggaattg agccgctttc tcatacactt ttgccaggtc tgaatacaac
     1561 gatcccggca tggaatcttt cgacggaatc tggtccatac ggttagacac gatggacaac
     1621 gcatcggcat acgaggtcat gtccgacaaa aggaccagca cctgttcgtt tttatctacg
     1681 gcaaaatatt ctgcggccgt caatgccata tcgggaacga gcagccgttc aacggctgga
     1741 ttttccgtcg tgttcataaa gctgatgatg cggtcgagcg cgccagcgtt gctgaacaca
     1801 tttttgtaat agaggtaatc gtcattcgtc atccccatcc cgccgagaat aattttatcg
     1861 gccttggcac gaagggccac cgttgccatt acctggttga aaggctggtc gggatcggca
     1921 aagaacggga ttttctgtcc cgagacgagc gtgttgttca ggtctatacc cgagatgccc
     1981 gtagcgatga gttccgacgg ttgtttcctt cttaccgggt taaccgatgg cccgccaatc
     2041 tggcgttctt ctccttcggg tacaggcccg ccgtcgatgg ggtcgccgta ggcattaaaa
     2101 aaacgtccgg caagttggtc tcccactttc agcgaaggcg cttttccgag aaagaccact
     2161 tctgcattgg tcgggatgcc ttccgttcct tcgaaaatct ggagggtaac ctcgtcgttg
     2221 atgattttca ctacctgggc caatcgtccg tctacggtgg ccagttcgtc ataacccaca
     2281 tcggtggctt ttacagaaca cgtggctttg gtgatctgtg tgatcttggt gtatattttt
     2341 tgaaatgctt tagccataat ttttcaattg ttaattaatt cctccagttg cctgtggtaa
     2401 tcgttgaatt tatccgattt gaactccgaa taattcatct gcctgcatac gttgatcagt
     2461 ttcttgaagt attccgctac ctggttgaaa ttttcaaatt cgaattcagc acgacatatg
     2521 tctactacca tatccaggat atacttctgc cgttcgatcg gtgttaccga atcaatttcg
     2581 tcaaaagcgt cctgttgcag gataacaaaa tcgatcaact ccgatttcca gaatgtgatg
     2641 tggtattcaa cggggacacc gtcgtcgccg agaatattaa tttgctcggc aatttccttt
     2701 ccccggagca accgcgtttt aatgtcgttg acctttgttg tccagtctcc ggatatctct
     2761 tttgagatat attcctcaaa ttcgggatat tcaatgtatt tggaataaga ttcgatgggg
     2821 ttgatggccg ggtagcgttt tctgtcggcg cgatcctgct cgagtgcgta ataacaacgg
     2881 gctactttct tcgtgttttc ggtaacgggt tctttcaggt taccgccagc aggcgacacg
     2941 gttccgataa aggtgatgga accttccttg ccgttattca gcgtaacgtg tccggcacgt
     3001 ccgtagaagt tcgaaatgat cgccgacaag tccatcggga aagcatcggg tccaggaagc
     3061 tcctccagcc ggttgctcat ctcgcgtaac gcttgcgccc aacgcgatgt ggagtcggcc
     3121 atcagcaaca ccttcagccc catggaacga taatattctc cgatggtcat ggccgtgtaa
     3181 accgaggctt cgcgtgcggc aacgggcatg ttggacgtgt ttgcaacgat aatggtccgc
     3241 tccatcagtt tccgtccggt atgcgggtca tcaagttcgg gaaattcggt gaaaatttcc
     3301 accacctcgt tagcacgttc accacaggca gcgataataa caatatcggc ctccgcttgt
     3361 ttggaaatgg cgtgctgcag caccgttttc ccagttccga aagcgccggg tataaatccg
     3421 gttccgcctt ccacaatcgg gttcagtgta tctatcgttc tcacgccggt ttccagcagt
     3481 ttaaacggcc tgggtttttc cttgtacagg gtaacgggca ctttcaccgg ccatttctga
     3541 atcatgttca gtttatgctc ctccccgttt ttatcttcaa ccaccgctac ggtgtggtaa
     3601 atgttgtact ctccttccgg tgcgatactt ttcaccttgt attcgctggt catcacgaac
     3661 gggaccatta tcttgtgggg ttggaaattc tcatccactt cacccaacca tgatcctccg
     3721 gaaacgttgt cgccaacctg tgctatgggc ttaaaagccc attttttttc ctcgtcaagc
     3781 ggaaaagtat attgtcccct tttcagaaag acaccctcca tcttgtccag atcgttttcc
     3841 aagccgtcca ggtttcgttc aagaagaccc ggtccgagca caacttcgag catatgtccc
     3901 cgaaactcca c
//
LOCUS       DAOSDR010000308         3839 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrimonas sp. isolate AMR_MDS_1826
            NODE_8372_length_3839_cov_3.666490, whole genome shotgun sequence.
ACCESSION   DAOSDR010000308 DAOSDR010000000
VERSION     DAOSDR010000308.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863355
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrimonas sp. (wastewater metagenome)
  ORGANISM  Petrimonas sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Dysgonomonadaceae; Petrimonas.
REFERENCE   1  (bases 1 to 3839)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3839)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:10:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,720
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 35
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3839
                     /organism="Petrimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8372_length_3839_cov_3.666490"
                     /isolate="AMR_MDS_1826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2023866"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..974
                     /locus_tag="PLI30_10960"
     CDS             <1..974
                     /locus_tag="PLI30_10960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009129911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI80110.1"
                     /translation="FRKDTADHPTLWKDPRGQREDGINPIMQLHYIFNLQVRFASGPM
                     KLMQSENDKMTDTPGFFQGVELFMDILRQADKPVEVLSFGSARVIAVAYNRAPELLKK
                     KIKRIHLSAGTAAPDYEMGSDAGANMIPGGEWNVALDVFAFTRLLRSPLPIALYACAG
                     KDGGFIKSTNNTYWQLKDMSFLKEMHPQLQCYIDFAFDKKQRTDFLKAMDKGSPYNEN
                     IKIQFDTFHVWESAIWLEVTNRKLVKDPAGNYKLVREKELKHSDTILESGLRPCRFSE
                     IRNDGRFIFEYVNHPSNTSIYYRADPEENEAALNVVIPELFKSYSPN"
     gene            983..2029
                     /locus_tag="PLI30_10965"
     CDS             983..2029
                     /locus_tag="PLI30_10965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016660986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HOI80111.1"
                     /translation="MKKISVIIAFLFLLAGGPGGAAQASCEEEKVNVIFDTDMGNDVD
                     DALALDMLYKYLELKKADLLGIMISKNNRFAAEFTDIMASWYGHTDIPVGIVKNGTDD
                     ELEAVNYAQIVSEMQENGAPVFERFGNSYELLPEASKLYRKILSTQPDHSVTIISVGF
                     FTNLAQLLETDADEFSDLNGKDLVTKKVKYLTVMAGSFGKNAIASYNVRKDIQSAAKV
                     FSEWPTRIVASPAEVGIKIRYPASSIMNDFNWTKLHPMVEAYKVYLPMPYDRPTWDLT
                     NLLYAIDNSYSYLEESAPGTIIVDEQGYTHFHSVTDGKHCYLSVSGDKVDVIRDYFVN
                     LIRQKPLKYKQDNE"
     gene            2077..3120
                     /locus_tag="PLI30_10970"
     CDS             2077..3120
                     /locus_tag="PLI30_10970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007211216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HOI80112.1"
                     /translation="MIKIYLAVITTFLMVGCSTKTQQSTENKESKMKVIFETDMGNDV
                     DDALALDMLYKYQDAGKIDLIGIMTNKESPYSAEYIDVMNTWYGYSQIPVGIIHQGAD
                     CEKDAINYAQSVCMLQDKDGKPVFMRSLSDYSQLPEAHMLYRKILAEQPDTSVTVISV
                     GFSTNLARLLETPADDLSPLTGKELVAQKVKLLCTMAGCFNNPNLFEYNVVKDIPAAK
                     KVFEEWPTRLVTSPFEVGIAINYPASSIENDFGWTPMHPMVEAYKSYQKMPYDRPTWD
                     LTSVLYSVEGPSYFTVSPAGTINVTEKGATEFSQQAKGNRYYLMVDSIQAANIKQHFI
                     ELIKKQPTHFNKK"
     gene            3126..>3839
                     /locus_tag="PLI30_10975"
     CDS             3126..>3839
                     /locus_tag="PLI30_10975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016660985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding protein"
                     /protein_id="HOI80113.1"
                     /translation="MKRYLLGIIALWLTYSCSEGGSGDLPPVDPPGPDTPTISMSKPE
                     FVFGFVGQLKYSYCPSTLKQEDGSVHMFFCGNPQNMIMVDNIYHVKINADGSKTSPKS
                     VLQPGTSSSWDDHHTCDPSVVEGRFTWENTTYKYAMFFLGNKYGVYYNEIGVAFSNEL
                     DADSWVKYPAQIVKKTWTSDSDQSLGGGGKSWGVGQPSVISLDGKGKLLLTYTVGDFS
                     GTRIVWSEGEFSDVANFTLT"
BASE COUNT         1154 a          831 c          899 g          955 t
ORIGIN      
        1 cattcaggaa agatacagcc gatcacccaa cattgtggaa agatccgcgc ggacaaaggg
       61 aagacggcat taacccaatt atgcaactcc actacatttt taacctccag gtcaggtttg
      121 ccagcgggcc gatgaaactg atgcaaagtg aaaacgataa aatgacggat actcccggtt
      181 ttttccaggg agtagagttg tttatggata tcctgaggca ggcagacaag cccgtggaag
      241 tgctctcttt tggttcggca agggtgattg cggttgctta caacagggca cctgaattgc
      301 ttaaaaagaa aattaaaaga attcacctca gcgccggaac ggctgcccct gattacgaaa
      361 tggggtcaga tgcaggtgcc aatatgattc ccggaggcga atggaatgta gcgctggatg
      421 tatttgcttt tacaagactg ttacggtccc ccctgcccat tgcactttat gcgtgcgctg
      481 ggaaggacgg tggcttcatt aaatcaacca acaacacgta ttggcaatta aaggatatgt
      541 cgttcctgaa agagatgcat cctcaactac aatgttatat tgattttgca tttgataaaa
      601 aacagcggac cgatttcctg aaggccatgg acaaaggaag cccatacaat gaaaacatca
      661 aaattcagtt tgacacattt catgtttggg agtccgccat ttggctggaa gtcacaaaca
      721 ggaagctggt taaagatcct gccggaaatt ataagttggt cagggaaaaa gaattaaagc
      781 attcggatac aatattggaa agcgggcttc gtccctgccg gttctctgaa atccgcaatg
      841 acggacggtt tatttttgag tatgtaaatc atccttcgaa tacatcaatt tactaccgtg
      901 ccgatccgga ggagaacgag gcggcgttga atgttgtaat tcccgagtta tttaaatcct
      961 attcgcccaa ttaaaacaca tcatgaaaaa aatcagcgtc ataattgcat tccttttcct
     1021 gctggccgga ggcccgggtg gagccgccca agcatcctgc gaggaggaaa aagtgaacgt
     1081 gattttcgac accgatatgg gtaatgacgt ggatgatgcc ttagctttgg atatgctgta
     1141 taaatatctc gagctaaaaa aggctgattt actggggata atgatcagca aaaacaaccg
     1201 gtttgcagct gaatttaccg atatcatggc ttcctggtac ggacataccg acattccggt
     1261 tgggatagtt aaaaacggaa ctgacgatga gctggaggca gttaattacg cgcagatcgt
     1321 gagtgaaatg caggagaacg gagctccggt ttttgaacgc ttcggaaata gttatgaatt
     1381 actgccagaa gcatcgaagc tgtaccgtaa aattttatcc acacaacccg accattcggt
     1441 tacgataatt tcggttggat ttttcaccaa cctggcgcaa ttgctcgaaa cggatgctga
     1501 tgaattttct gatctgaacg gaaaagacct ggtgactaaa aaagtaaaat acctgacggt
     1561 aatggccggc agttttggca aaaatgcaat tgccagctac aacgtcagga aagatattca
     1621 atccgcagca aaagtatttt cagagtggcc aacccgtatt gtggcctctc ctgccgaggt
     1681 cgggataaaa atcagatatc ctgcatccag cataatgaac gatttcaact ggacaaaact
     1741 acatccgatg gttgaggcat acaaagtcta tctacccatg ccttatgacc gtccgacctg
     1801 ggatttgaca aacctgctgt atgcaattga caacagctat agctatctgg aagagtctgc
     1861 tccgggaacc attattgtgg acgaacaggg atatacccat tttcacagcg taacggatgg
     1921 caaacactgc tacctaagcg tgtccgggga taaggtggat gtaatcaggg actactttgt
     1981 aaacctgatc agacaaaaac cattgaaata caagcaagac aatgaataga accatttgat
     2041 tttaatagaa atttataaca gtaaaaaaca accgagatga ttaaaattta tttagccgta
     2101 attaccacat ttctgatggt tggatgctct accaaaacac agcaatcaac ggaaaacaaa
     2161 gaatcaaaaa tgaaagtgat ctttgaaaca gatatgggca atgatgtgga tgatgccctg
     2221 gcgctcgaca tgctttacaa atatcaagat gccggaaaaa ttgatctgat cggcattatg
     2281 accaataagg aaagcccata ctctgcggag tacatcgatg tgatgaacac atggtatggt
     2341 tattctcaaa tccctgttgg gattattcac cagggggcag actgcgaaaa ggatgccatc
     2401 aactatgccc aaagcgtttg tatgctacag gataaggatg gaaagcctgt atttatgcga
     2461 tctctgtcag attattccca gttgccggag gcacacatgc tctaccgcaa aatactggca
     2521 gaacagcctg acacttctgt tacggttatt tccgtaggct tttccacaaa tctcgcccgc
     2581 ttgttggaaa caccggccga cgacctctct cctctgaccg ggaaagaact ggtggcccaa
     2641 aaagtgaagc tattgtgtac gatggcaggt tgctttaata acccaaattt atttgagtac
     2701 aacgttgtga aagacattcc ggctgccaag aaggtttttg aagaatggcc aacccgcctg
     2761 gtaacctccc cgttcgaagt tgggatagcg atcaactacc ccgccagcag cattgaaaac
     2821 gactttggct ggacgccaat gcatccgatg gtggaagcct ataaaagcta ccaaaaaatg
     2881 ccttatgacc gccccacctg ggatttaact tccgtattgt attcggtgga aggaccttca
     2941 tattttaccg tctcgcctgc aggaacaatc aatgtaacgg agaaaggggc tacggaattt
     3001 tctcagcagg caaagggaaa tcgctattat cttatggttg attccattca ggctgccaac
     3061 atcaaacaac atttcataga gttgatcaaa aagcaaccga ctcatttcaa taaaaagtaa
     3121 acaaaatgaa aagatactta ttggggatca ttgcgttatg gctgacctac tcctgttccg
     3181 aagggggatc gggtgacctt ccgcctgtcg acccaccagg cccggatact ccgacgatat
     3241 ccatgtcgaa gccggaattt gtctttgggt ttgtgggaca actcaagtat tcatactgtc
     3301 ccagcaccct gaaacaggaa gatggcagtg tccatatgtt tttctgcgga aacccgcaaa
     3361 acatgattat ggtcgacaac atttaccatg tcaaaataaa tgcggatggt tctaaaacat
     3421 ctccgaaaag tgttctgcag ccgggcacgt caagttcgtg ggacgatcat cacacctgtg
     3481 atcccagcgt tgttgaaggc cggtttacct gggagaatac aacctataaa tatgcgatgt
     3541 tctttttggg caataaatac ggcgtctatt acaacgaaat tggcgtagcc ttcagcaatg
     3601 aactggatgc agatagctgg gtaaaatatc ctgcgcaaat agtgaagaag acctggacaa
     3661 gcgacagcga ccagtcactt ggaggaggtg gtaaatcttg gggagttggc caaccttcgg
     3721 tcatatcatt agatggaaaa ggaaaacttc ttctgaccta tactgtcggt gattttagcg
     3781 gcacccgcat cgtctggtcc gaaggggaat tcagtgatgt ggccaacttc acgttaacc
//
LOCUS       DAOSDR010000309         3836 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrimonas sp. isolate AMR_MDS_1826
            NODE_8387_length_3836_cov_2.616239, whole genome shotgun sequence.
ACCESSION   DAOSDR010000309 DAOSDR010000000
VERSION     DAOSDR010000309.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863355
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrimonas sp. (wastewater metagenome)
  ORGANISM  Petrimonas sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Dysgonomonadaceae; Petrimonas.
REFERENCE   1  (bases 1 to 3836)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3836)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:10:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,720
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 35
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3836
                     /organism="Petrimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8387_length_3836_cov_2.616239"
                     /isolate="AMR_MDS_1826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2023866"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            125..2206
                     /locus_tag="PLI30_10980"
     CDS             125..2206
                     /locus_tag="PLI30_10980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019244638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HOI80114.1"
                     /translation="MMTANMSNFCKLLFATLLLLFVLLPRSSTAQQKIRFSNKPVEIT
                     RWIHEAFAKGKIPPFSFELDGKESSDFIKSWDYSFEKRVSTEVNVVRYRITYESKQAG
                     LRIDCEVEGYTDYDAVKWLLHFTNLQDEDSKVISQVKVINVDFEYACSGDFILHTADG
                     NHISKADFHPRSLVMNSGEEHHFFPEGGRSSETSFPFFNIESPANQGVVFAIGWSGTW
                     ISDFVKKTDSTFSFNSGMKRFESYLKSNETFRTPSMCLLFWNAENRMEGHNKFRRFVL
                     AHQSRKINGKFAAYPLSSGFNYHDPEPCTEYSCLTEDYAIAMIRRYTQFGLIPEVYWL
                     DAGWYSGAANFEQGQTWANTVGNWTVDTTRFPRGLKPIAEEAHKVGAKFMVWFEPERV
                     IEGTQWAREHPEWMLRHGEGDWLLFDLGNKDARKWMTRKIIDLIRDNGIDYYRQDFNM
                     RPDVYWEANDEPGRVGMKEVRHIEGLYAFWDDLLTEFPNLLIDNCASGGRRLDMETIP
                     RSAPLWRSDYYHYNDPDGYQGHTYGLNFFLPLHGTGILQTDRYSFRSSMSSALIYNWK
                     ITDKNVSYIDMQNCLKEFASVRPYYYEDYYPLVGIEDLTRDNIWIAYQLHRPTDESGI
                     IVAFRREKSPDNRLTVKLGGLKKDGNYLITSMDDESFKCFKSSKELNEGFDLMIEEPR
                     GSLLLKYSIVK"
     gene            2219..>3836
                     /locus_tag="PLI30_10985"
     CDS             2219..>3836
                     /locus_tag="PLI30_10985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019538993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HOI80115.1"
                     /translation="MKKYIASWVILCLVWMAAPAYGQSLSRFGNKNVKVGDWVDQQFR
                     KGTTPPFSFRYNGNHSSDFIGKWQFTAKKGGQNAQGVVNNIYSWQDRKTGLNVTCDLK
                     YYPEYEAVEWVLHFTNGSGADSPLITQVRVIDMDWRYGADGDLILHYANGSSASRGDF
                     NPREQVLAGGEPIELSPLNGRSSDHAFPFFNIESTTGQGTLLSVGWTGTWYARFEKNG
                     DNGLSLHAGMKRLDSYLHANESIRTPSICLLFWRGEDRMTGHNAFRRFMINHQAPKIN
                     GKAAVYPVSTSFNYGDPAPCNEYTCLTTDYALAMINRYKQFKLVPEVFWLDAGWYKHA
                     NEVENNKNWANTVGNWEVDTDRFPGGLKPISDAVHEAGAKFMVWFEPERVIKESKWGR
                     EHKEWMLDAKGSDAYLFDLGNKEALQWFCNYIGDFMEKNGIDYYRQDFNMRVDNFWEE
                     NDEPGRTGIREIRHIEGLYAFWDYLLQRFPEAIIDNCASGGRRLDFETMKRSAPMWRT
                     DYSYGEPVGYQTHTYGLNFFLPLTGTGVQKS"
BASE COUNT         1092 a          749 c          994 g         1001 t
ORIGIN      
        1 gttgtctccg acagttttgg tacccagaca gctgccatga tgctgatcac cttggtgtgg
       61 ctctacatgg tctggctgat tcgaacggtg agcaataagc aagaaaaatc attaacaaat
      121 tgacatgatg actgcgaata tgtcgaactt ctgcaaattg ttgtttgcaa cgctattatt
      181 acttttcgtt cttctaccaa gatcgtctac tgcacaacaa aagatcagat tcagcaataa
      241 accggtggag ataacccgat ggattcacga agcgtttgcg aaagggaaga tccctccatt
      301 ttcgtttgaa ttagatggta aagagtcttc agatttcatc aaatcttggg attactcttt
      361 tgaaaaacgg gttagtactg aagtgaatgt ggtaagatat cgtattacct atgaaagcaa
      421 acaggccggt ttgagaattg actgtgaggt tgaggggtat accgattacg atgctgtcaa
      481 gtggttgtta catttcacaa acttacaaga tgaagattca aaagtgattt cgcaggtgaa
      541 agtgatcaat gtagattttg agtatgcgtg tagcggtgat tttattttgc atacggctga
      601 cggtaatcat atatcgaaag ctgattttca cccgaggtcg ttggtgatga attcagggga
      661 agagcatcat ttttttccag aaggaggtcg ctcatctgag acatcgttcc cattctttaa
      721 tattgaatca cctgccaacc agggggttgt ttttgcaatt ggatggtctg gtacctggat
      781 aagcgacttt gtgaaaaaaa cagacagtac tttttctttc aactctggga tgaaacgatt
      841 cgaaagttat cttaagtcga atgagacttt tcgtacgcct agtatgtgcc tgctcttttg
      901 gaatgctgag aatcgaatgg aaggacacaa caaattcaga aggtttgtgc ttgctcatca
      961 aagtcgaaaa attaatggaa agtttgcagc atatccgctc tctagtggtt tcaactatca
     1021 cgatcctgaa ccctgtaccg aatattcttg cctgacggaa gattacgcga tcgccatgat
     1081 acgcagatat acacaatttg gtctcatacc cgaagtttat tggcttgatg ccggttggta
     1141 tagtggagcg gctaactttg aacagggtca gacatgggcc aatacggtgg gtaactggac
     1201 ggttgataca acgaggttcc cgaggggact gaaaccaatc gctgaagagg cacataaagt
     1261 tggagcaaaa tttatggtat ggtttgaacc ggagagagtg attgaaggaa cacaatgggc
     1321 cagagaacac ccggaatgga tgctacgaca cggagaagga gattggttac tttttgatct
     1381 agggaacaag gacgcaagaa aatggatgac ccggaagatc atcgatctga ttcgagacaa
     1441 cggtattgat tactatcggc aagattttaa catgcggccg gatgtttatt gggaggcaaa
     1501 cgatgaaccg ggacgagtag gaatgaagga ggttcgtcat attgaaggac tatacgcttt
     1561 ttgggatgat ttattgacag aattccctaa cttgttgata gataattgtg caagcggagg
     1621 acgacgtctg gatatggaga ccattccgag aagtgctcca ctatggagaa gtgattatta
     1681 tcactataac gatccggatg gataccaggg acatacatat ggacttaatt tctttcttcc
     1741 attacatggt acaggtatat tgcaaacaga ccgttattca tttcgttcga gcatgagcag
     1801 tgcattgatt tataactgga agataacaga caagaatgtc tcttacatcg acatgcagaa
     1861 ttgcctgaaa gaatttgctt cggtaagacc ttattactac gaggattatt atccgcttgt
     1921 cggaattgaa gacttgacac gagacaacat ctggattgcg tatcaattac acagacctac
     1981 tgatgagagc ggaatcattg tagctttcag acgtgagaaa tcacctgata atcgattgac
     2041 agttaagttg ggaggtttaa aaaaggatgg aaactatttg attaccagta tggatgacga
     2101 gtcgtttaaa tgctttaaga gtagcaagga attgaacgaa ggttttgatt tgatgattga
     2161 ggagccaaga ggctcattgt tattaaaata ttcaattgta aagtagatat ttgtgtatat
     2221 gaaaaagtat attgcttcct gggtaattct gtgtttggtg tggatggcgg ctccagccta
     2281 tggacagtcg ctttcccggt tcgggaataa aaatgtgaag gtgggtgatt gggtagatca
     2341 gcaattcaga aaagggacta cccctccctt ctcgttccgc tacaacggga atcactcatc
     2401 cgattttatc gggaaatggc agttcacagc caaaaaaggg ggccagaatg ctcagggtgt
     2461 ggtgaacaac atctacagct ggcaggaccg caaaaccggg ctgaacgtga cctgcgacct
     2521 gaagtattat ccggaatacg aagcggtgga atgggtcctt catttcacga atgggtcggg
     2581 cgctgactcg cctttgataa cacaggtgag ggtgatcgat atggattgga ggtacggcgc
     2641 tgacggtgat cttatcttac actatgctaa cgggagttct gcctcgagag gggacttcaa
     2701 cccgcgtgaa caagtcctgg ccggagggga acccattgaa ctatctccct tgaatggtcg
     2761 ctcttccgat cacgcctttc ccttcttcaa cattgaatcg acaaccggac agggtaccct
     2821 gttgtcggtg ggatggaccg gtacctggta tgcccggttt gaaaagaatg gggacaacgg
     2881 gttatccctc catgcgggga tgaaacggct ggatagctac ctgcatgcca acgaatctat
     2941 tcggacacca agtatttgcc tgttgttctg gagaggggag gatcgcatga ccgggcacaa
     3001 cgccttccga cggttcatga tcaatcacca agcaccaaaa attaatggaa aggctgcggt
     3061 gtatccggtt tctaccagct tcaattacgg tgatcccgcc ccctgtaatg aatatacctg
     3121 cctcactacc gattatgcat tagccatgat caatcgctac aagcagttta aactggttcc
     3181 cgaagtgttc tggctggacg ccggatggta taaacacgcg aatgaagttg agaataataa
     3241 aaactgggcc aataccgttg gcaattggga agtggatacg gacaggtttc ccggcggcct
     3301 aaaacccata tcggatgcgg ttcatgaagc tggtgccaaa ttcatggtgt ggttcgaacc
     3361 ggaaagggtg atcaaagaat ccaaatgggg cagggagcac aaagagtgga tgctggatgc
     3421 gaaaggaagt gacgcctacc tgttcgatct gggaaataag gaggctttgc aatggttctg
     3481 caactatatc ggtgacttca tggaaaaaaa cggaatcgat tattatcgcc aggattttaa
     3541 catgagagtc gataacttct gggaagaaaa cgatgaaccg ggtcgaacgg ggataaggga
     3601 aattcgccat atcgaagggc tctatgcttt ctgggactac ctgctgcagc gatttcccga
     3661 ggcgatcatt gacaactgcg caagcggggg caggcgcctc gactttgaaa ccatgaaaag
     3721 aagcgctccc atgtggcgga ccgattacag ttacggggaa ccggtcgggt accagacaca
     3781 tacctacgga ctgaacttct tccttccgtt gaccggtaca ggtgtacaaa aaagcg
//
LOCUS       DAOSDR010000310         3828 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrimonas sp. isolate AMR_MDS_1826
            NODE_8411_length_3828_cov_4.032070, whole genome shotgun sequence.
ACCESSION   DAOSDR010000310 DAOSDR010000000
VERSION     DAOSDR010000310.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863355
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrimonas sp. (wastewater metagenome)
  ORGANISM  Petrimonas sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Dysgonomonadaceae; Petrimonas.
REFERENCE   1  (bases 1 to 3828)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3828)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:10:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,720
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 35
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3828
                     /organism="Petrimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8411_length_3828_cov_4.032070"
                     /isolate="AMR_MDS_1826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2023866"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1815
                     /locus_tag="PLI30_10990"
     CDS             <1..1815
                     /locus_tag="PLI30_10990"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HOI80116.1"
                     /translation="QQKVQENVDLQSGYYVVYGGQYQNLKNASTRLMIIVPIALALIF
                     LLLNFAFNSLKETIIIFSAIPLSIVGGILLLWLRGMPFSISAGVGFIALFGVAVLNGI
                     VLIEHFKELRHKNGGMKMHDIIVQGSVDRLRPVVLTAASAAMGFLPMAISTSAGAEVQ
                     RPLATVVIGGLATSTFLTMIVLPLLYGYFMNDERRGRKNRSRKRNVTKAKFWLVSVLM
                     TVSVTAFSQSSELSLEEAIATGIANNRQLKTFELQVQRSKAFVPTALNIGKTGVYYGR
                     DANNIAENNHPLYVVGVSQEINFPTVYAAQYKANRAGVSVSQAAYELERQRLSKNITQ
                     AYYQVIYLQNKQQQYRFIADIYRKFSRSADAQYKQGEIAYLEKLNATAKQQQVDIQQK
                     QIDADLQIAYRQLSLFLQPKEDIRVPLQELEPVLLNKDIQNNAGLKLANEQLSYERAL
                     LDVERNLCLPDISVEVFNGTNRFANAKSYWGWQVGISVPLFFGEQKARVASQKHSVMM
                     AGYSRQQYEVRYNSTYEQLRNELEKYRQNIDYYQTSGKQISDELIKFATSSYEIGEID
                     FFRYIQSLENATQLRLDYLDNLAKYNQTVIALNYLIVE"
     gene            1865..2998
                     /locus_tag="PLI30_10995"
     CDS             1865..2998
                     /locus_tag="PLI30_10995"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HOI80117.1"
                     /translation="MRFYKHFFWGVLVSVALLGCGNKKSGSEAGDSTGDSALVAISNR
                     QFEAMQMEFGELQEAQFHESVKVNGHVISSVRGQAQVTSRIPGTIRIISRELGDFVSA
                     GSVLCTVESNDFIVLQQDFATVSNQFNEAKKAYERLQTLYRDSIAARKDFIAAESAYN
                     SLLAQYNGMKHRLSLLNVDPAQIEKGDFYRILEVRSPIDGYITQVNSMIGEYVTPEKM
                     LMTIVNANQFYLQLDVYEKDIAQLAVGQNIWFYNSSNPSEKFSAKLTRTGKSIDPEKK
                     TIVCTAQIESINSGFVNNMYVEAEVSVKEYTAKGIPGDALEALADGTYRIYLLEKKSG
                     DNIYLKSVSVTTGLTSNNMVEIKTPLPTQQVLVKGVYNLPATE"
     gene            3060..>3828
                     /locus_tag="PLI30_11000"
     CDS             3060..>3828
                     /locus_tag="PLI30_11000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI80118.1"
                     /translation="MELTRKIYPLCLLGVLLLVSCRSAKLSDARAQYIRGEYFVASET
                     YRKVYRNIKPDQHPLRGVVAYEMAEAYRRMNVASRSANAYANAIRYKYPDTLVYLRYA
                     QMLHKEGRYLQAQKAYEDFLALDPVNLLGISGLQGVKDAVEKKKTPSRYVVKRMDLFN
                     SNRGEFSPAFAKDDAVLYITSSREDARGDSISNITGMKNNDIFIVTRNDKGGWMKPEI
                     LESEINTRFDEGTPSVSADGNYLFYTFSPVYSHRATTP"
BASE COUNT         1097 a          792 c          967 g          972 t
ORIGIN      
        1 cagcaaaaag tgcaggagaa cgtagattta caatcggggt attatgtggt ttatggaggc
       61 cagtatcaga accttaagaa tgcatctacc cggttgatga ttattgtgcc tatcgccctt
      121 gcacttatat ttttattgtt gaacttcgct ttcaattcgt tgaaagaaac gataataatc
      181 ttttcagcca ttccgctttc tattgtcgga ggtatactcc tgctctggtt aaggggaatg
      241 ccgttcagca tctcggccgg ggtagggttt attgcgttgt ttggtgtggc tgtgctgaac
      301 ggaattgttt taattgaaca ctttaaagag ttgcgccata aaaacggagg aatgaaaatg
      361 cacgacatta ttgtacaagg ctctgtcgac aggcttcgac ccgttgttct tacagcggct
      421 tctgctgcga tgggattcct gcctatggct atttccacat cggcaggagc ggaagttcaa
      481 cgcccgctgg ccacggttgt tattggtggg ttagctactt ccactttcct tacgatgatt
      541 gtcttgccgc tgctttacgg atattttatg aatgacgagc gcagagggag aaaaaaccgt
      601 tcaaggaaaa gaaatgtgac gaaagcgaag ttttggcttg tctcggtttt gatgacagtt
      661 tctgttacag ctttttccca atcgagtgaa ctttcgctgg aggaagccat cgcaaccgga
      721 atagccaaca accggcaact gaaaactttc gaattgcagg tacagcggag caaggcgttt
      781 gtccccacag ctttgaatat agggaaaacc ggggtgtatt acggcaggga tgccaataac
      841 attgccgaaa acaatcatcc gctatatgtg gtcggggtat cgcaggaaat aaatttccct
      901 accgtttatg ccgcgcaata caaggccaac cgggcagggg tttcggtttc acaagcagct
      961 tatgaacttg aacgacaaag gctcagtaag aacataacgc aagcgtatta tcaagtgata
     1021 tatctgcaaa acaaacaaca acagtatcgt tttatcgccg atatttaccg caagttttca
     1081 cgatcggctg atgcgcaata caagcaaggt gaaattgcgt acctggaaaa actgaatgca
     1141 actgctaaac aacaacaggt tgatattcag caaaaacaaa ttgacgccga cctgcagatc
     1201 gcttaccggc agttaagctt gtttttacag cccaaagaag atatccgggt tccacttcag
     1261 gaactggagc cggttttgtt gaacaaggac atccagaaca atgccgggct caaactggcc
     1321 aatgaacaac tcagctacga gcgtgcctta ctggatgttg agagaaattt gtgtctgccc
     1381 gatattagcg tcgaggtttt taacggaacg aaccggttcg caaacgcgaa aagttattgg
     1441 ggctggcagg ttggaatttc tgtcccgctg ttctttggag aacagaaagc cagggtcgct
     1501 tcgcaaaaac acagcgttat gatggccggt tactccaggc agcagtatga ggtgcggtac
     1561 aactctacgt atgaacaact gcgcaacgaa ctcgaaaaat accgtcagaa tattgattac
     1621 taccaaacgt cgggaaagca gatcagtgat gagctgatta aatttgccac gtcaagttac
     1681 gaaatagggg agattgactt cttccgttat atacagagcc tggaaaatgc tacgcaattg
     1741 aggttggatt atttagacaa cctggcgaaa tacaaccaga cggtaattgc gctcaattac
     1801 ttgatagttg agtgaaccgg cggataaagg gcaagaggta agaaaaacgt aaaaagacag
     1861 caagatgcga ttttataaac atttcttttg gggagttttg gtttccgtag ctcttttggg
     1921 ttgcggtaac aaaaagtcgg gtagcgaagc cggagattct accggagatt ctgcgttggt
     1981 agcaatttcc aaccgccagt ttgaagcgat gcaaatggaa tttggtgaat tgcaggaagc
     2041 gcaattccac gaaagtgtga aggtgaacgg gcatgtcatc tcatcggtaa gagggcaggc
     2101 ccaggttacc agccgaattc cggggacgat aaggattatt tcccgtgagt tgggtgattt
     2161 tgtctctgcg ggaagtgtgc tttgcactgt cgaaagtaac gattttattg tgctgcagca
     2221 ggattttgct acggtaagca atcagttcaa cgaagcaaaa aaggcttacg aacgattgca
     2281 aacgctttat agggacagca tcgcagcccg taaagatttc atcgctgccg aaagtgctta
     2341 caacagtttg ttggcacaat acaacgggat gaaacatcgg ttgagtctgc ttaacgtaga
     2401 tccggcgcaa atagaaaaag gggattttta tcggatactc gaagtccgtt cgcctattga
     2461 cggatatatt acgcaggtca acagtatgat cggagaatac gttacgcctg aaaaaatgct
     2521 gatgactatt gtcaatgcca atcagttcta tcttcaactg gatgtatacg agaaggatat
     2581 cgcacaattg gctgtcggtc aaaatatctg gttttataac tccagtaatc cgtccgagaa
     2641 gttcagtgcg aaactgacca gaacagggaa atctatcgat ccggaaaaga aaaccattgt
     2701 gtgtacagcg caaatagaat cgattaacag cggttttgta aacaacatgt atgttgaggc
     2761 agaggtgagt gtgaaagaat atacggcaaa agggattccc ggggatgccc ttgaagcgtt
     2821 ggcggacgga acgtaccgga tttatttgct tgaaaagaaa tcgggagata atatatacct
     2881 taaatccgtc tccgtcacca ctgggttgac atccaacaac atggtggaga taaaaacgcc
     2941 gcttcccaca caacaggttt tagtaaaagg agtatataac cttccggcaa cggaatagtc
     3001 cccacggttt tcacacaata tttccgtcaa aaacgagtta ttaaggaaaa caaccggatt
     3061 tggaactaac tcgaaaaata taccctttat gcctcttggg ggtgctatta ctggtttcgt
     3121 gccgctcggc aaaactgagc gatgcccgtg cacaatacat tcggggagag tattttgtgg
     3181 cttctgaaac ctatcggaaa gtgtacagga acattaagcc tgatcagcat ccgctgcgcg
     3241 gagtggtagc ttacgaaatg gcggaggcct atcgccgaat gaatgttgct tcccgttcgg
     3301 ctaacgcgta tgccaatgcc atccggtaca agtatcccga tacgctggtt tatttgcggt
     3361 atgcgcaaat gttgcacaaa gaaggaagat acctacaggc acaaaaggct tacgaagatt
     3421 ttctggctct ggatccggta aatttattgg gaataagtgg attgcagggg gtgaaggatg
     3481 cggtggagaa aaagaaaacg ccgtcgcgtt atgtagtaaa acggatggac ctgttcaact
     3541 cgaaccgcgg agagttcagt ccggcgtttg ctaaagacga tgccgtgctt tatatcacct
     3601 cttcgcgcga ggatgctcgg ggtgattcga tcagtaacat cacggggatg aagaacaacg
     3661 atattttcat tgtcaccaga aacgataagg gcggctggat gaagccggag attttggagt
     3721 cggaaatcaa tacccggttc gatgaaggga caccctcggt ttccgcagat ggcaattacc
     3781 ttttctatac cttctctccc gtttattctc accgggcaac cactccca
//
LOCUS       DAOSDR010000311         3810 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrimonas sp. isolate AMR_MDS_1826
            NODE_8462_length_3810_cov_5.029294, whole genome shotgun sequence.
ACCESSION   DAOSDR010000311 DAOSDR010000000
VERSION     DAOSDR010000311.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863355
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrimonas sp. (wastewater metagenome)
  ORGANISM  Petrimonas sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Dysgonomonadaceae; Petrimonas.
REFERENCE   1  (bases 1 to 3810)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3810)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:10:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,720
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 35
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3810
                     /organism="Petrimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8462_length_3810_cov_5.029294"
                     /isolate="AMR_MDS_1826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2023866"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2564)
                     /locus_tag="PLI30_11005"
     CDS             complement(<1..2564)
                     /locus_tag="PLI30_11005"
                     /inference="COORDINATES: protein motif:HMM:NF014758.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HOI80119.1"
                     /translation="MIKKVLNVNGVQRTLIVDQEALLSDVLRKQLHLTGTKVGCGTGQ
                     CGACNVIVNGKLIRSCVTKMKRIDNFSEITTIEGIGTPLNLHPLQKAFIFHHAIQCGF
                     CTPGFIVSAKALLDENPKPTREDVRDWFQKYRNACRCTGYIMIVDAVMDAAKVLRGEM
                     SEAALEYKQPADGRVYGTRMPRPTFVAKACGLWDFGEDMATQLPENTLQCALVQPKVS
                     HAIIKGIDTTEAEKMPGVFKVVTHKDVKGKNRITGLITFPTNKGDGWDRPILNDDKIY
                     QYGDVVAIVCADTKENAQAAVEKVKVDLEVLPAYMNAPAAMAEDAIEIHPGTPNVYYT
                     CYGKKGADVKPIIEKAAHTFTGEYYLQRQPHMPIEPDVGFAYIDDQGRCVVHSKSIGI
                     HLHLYMIAPGLGLEPDQLVIVQNPTGATFGYKFSPTMEALVGAAAMATGRPCFLGYDW
                     YQQQTYTGKRSPSYLRGTIACDAEGKLLAIDASWDLDHGPYSEFGDLVTLRGQQFVGA
                     GYDIPNLNTEGHTVCTNHGWGSAFRAYGSPQAFLFGESIVDELARKAGFDPFEFRYKN
                     IYRPGATTPTGQVPEVFCFEEMFNLMRPKYQEAKAKAKLESTDTVKRGVGVSLGIYGC
                     GLDGPDTSNAWAELLPNGDVMIGNAWEDHGQGSDMATVAFAHEALRALNLKPSQIKLS
                     MNDTSLHPNAGPSGGSRQNVVTGNAIVAACRLLLNAMKKSDGTFRTYDEMVKEKIETK
                     YTGSWTTPCTHQDDNGIGKPFCIYMYALFCSEVAVDITTGKVEVVKMTYVGDNGTITN
                     RGTVDGQVYGGLVQGIGLALTEDFEDLKKHTTLIGSGFPFIKDVPDNIELMYVESPR"
     gene            3436..>3810
                     /locus_tag="PLI30_11010"
     CDS             3436..>3810
                     /locus_tag="PLI30_11010"
                     /inference="COORDINATES: protein motif:HMM:NF012492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HOI80120.1"
                     /translation="MSFQTFDFHPHIAAGVEAAGYVNPTAIQSQAIPPVLAGRDVMGL
                     AQTGTGKTAAFALPILHRLMKGDRGKVRALVVAPTRELAEQIHQSIENLGRKTRLRSV
                     TVYGGVKINPQTEKLKRGAEIVV"
BASE COUNT          825 a          988 c         1080 g          917 t
ORIGIN      
        1 cgaggagatt caacatacat taattcgatg ttgtcgggta catccttgat gaacgggaag
       61 cctgagccga taagggtggt gtgcttcttc aggtcttcga agtcctcggt gagggccaga
      121 ccgataccct ggacgaggcc accgtatacc tggccgtcaa cggtgccgcg gttggtgatg
      181 gtgccgttat cgcccacgta ggtcatcttc acgacttcaa ccttgccggt ggtgatatcg
      241 acggcgactt cggaacagaa cagggcgtac atgtagatac agaaaggctt gccgattccg
      301 ttgtcgtcct gatgtgtaca cggggtggtc caactgccgg tgtacttggt ttcaatcttt
      361 tccttgacca tttcatcata agtacggaag gtaccgtccg acttcttcat ggcgttgagg
      421 agcagtctgc atgccgcgac aatggcgttg ccggtaacaa cgttctgacg gctgccgccg
      481 gaagggccgg cattcgggtg gaggctggtg tcgttcatgg acagcttgat ctgcgacggt
      541 ttcaggttga gggcacggag cgcctcgtgg gcgaatgcca ccgttgccat atcggagccc
      601 tggccgtggt cttcccatgc attgccgatc atgacgtcgc cgttgggcag gagttcagcc
      661 caggcattgg aggtgtcggg gccgtcaagg ccgcagccgt agatgccgag ggaaacgccg
      721 acgccgcgct tcaccgtgtc ggtggactca agcttggcct tggctttggc ttcctggtac
      781 ttgggacgca tgaggttgaa catctcttcg aagcagaaca cttccggaac ctggccggtc
      841 ggggtggttg cgccgggacg gtagatgttc ttgtaacgga attcaaacgg gtcgaatccg
      901 gccttgcgag caagttcgtc gacaatggat tcgccgaaca ggaatgcctg cggagaaccg
      961 taagcgcgga acgcggaacc ccagccgtgg ttggtgcaaa cagtgtggcc ttcggtgttc
     1021 aggttcggaa tgtcatagcc ggcgccgacg aactgctggc cgcgcagggt gaccaggtcg
     1081 ccgaattcgg aataggggcc gtggtcaaga tcccagcttg cgtcgatggc gagcagcttg
     1141 ccttccgcgt cgcaggcaat tgtgcccctg agataggaag gtgaacgctt gccggtgtag
     1201 gtctgctgct gataccagtc atagcccagg aagcagggac ggccggtcgc catggctgcg
     1261 gcgcccacca gggcttccat ggtcgggctg aacttgtagc cgaaggtggc gccggtcggg
     1321 ttctggacga tgacgagctg atccggctca aggccgaggc ccggggcgat catgtacagg
     1381 tgcaggtgga taccgatgga cttggagtgg accacacacc tgccctggtc gtcgatgtag
     1441 gcgaatccga catccggctc gatgggcata tggggctgac gctgcaggta gtattcaccg
     1501 gtgaatgtat gtgccgcctt ttcgataatc ggttttacat ccgcgccttt tttgccgtag
     1561 caggtgtaat agacgttcgg cgtgcccgga tgaatttcga tggcgtcttc ggccatggca
     1621 gccggagcgt tcatgtaggc aggaaggact tccaggtcaa ccttgacttt ctcgactgct
     1681 gcctgtgcgt tttccttggt gtcggcgcat acgatggcga caacgtcacc gtactggtaa
     1741 atcttgtcgt cgttcaggat aggacgatcc cagccgtcac ccttgttggt ggggaaagtg
     1801 atgaggccgg tgatgcggtt tttgcccttg acgtccttgt gggtaacgac cttgaacacg
     1861 cccggcattt tttccgcttc ggtggtgtcg atgcccttga tgatggcgtg ggaaaccttg
     1921 ggctgtacca gtgcgcactg cagggtgttc tcgggaagct gggtagccat atcttcgccg
     1981 aaatcccaca gaccgcaggc cttggctacg aacgtgggac gcggcatgcg ggtgccgtag
     2041 accctgccgt cggccggctg cttgtattcg agagctgctt cggacatctc gccacggagc
     2101 accttggcgg catccatgac cgcgtcgacg atcatgatgt aacctgtgca gcgacaggcg
     2161 ttgcgatact tctggaacca gtcgcgaacg tcttcgcggg tcggtttcgg gttttcgtcc
     2221 aggagcgcct tggcggaaac gatgaagccg ggggtacaga agccgcactg aatggcgtga
     2281 tgaaagatga acgccttctg aagaggatgg agattgagcg gtgtgccaat cccttcaatt
     2341 gttgtgatct ctgaaaagtt gtcgatacgc ttcatcttgg taacgcagga acggatcagc
     2401 ttgccgttca cgatcacgtt gcaggcgccg cactggccgg tcccgcatcc aactttggtt
     2461 ccggtcagat gaagctgctt gcgcagcaca tcggacagga gggcttcctg gtcaacgatc
     2521 agcgtccgtt gtacgccatt gacattgaga accttcttaa tcatacattc tcctcctttt
     2581 ggtatgttta gacgggcagt gcccggttaa acgcgcgttc catggaagcc cgccttttcc
     2641 atgggcgaca agaaccgttt tttcccacaa ggacacagga cggtcgattt ttcttttcag
     2701 tttgccgtca tccctcagcc acagtggccg aaacggaaaa acccgcacca atactgaaga
     2761 gaactcagta tcaatgcggg tttcgttagg tccgccgaag gcggaggacg aaataggcgc
     2821 ttgtcttgtt tttattacct tattgttact ttactgttaa tcggaccgca gattcatttt
     2881 actgtaattt aatttttaaa atcttccatc gagcgttatt tatcatatta cacagcgagc
     2941 ttgcaagcga tatttcaatt taattgaaaa tttgtccttc aaaacggata gctgtccttt
     3001 gcgctatttc tcaataaata tttcgaagat cgttttgctt acttatttat tcataatatc
     3061 aaatacttta aaaagtgtac cgcatcacaa tccgattcta tttattgcca cttttttaca
     3121 ccccaacaca atattttaat tttactaaat attacataaa gcgaattttt cggacgagca
     3181 ttccagctga aatcatattt ttcatattaa taaatatttg aaatactcac tgaacaggaa
     3241 catgggatca aatcaaaaca ttccataatg acaaacgccg cacatccgaa acgcggcaaa
     3301 ggaatatctt gacatgaccg gcccgaactg ctacggtcat cccaacgaaa cgaatcagcc
     3361 ggaacacaac atcactgtaa gccccggaaa tctccggggc tttttttata ccctcacaca
     3421 atagtaagga gtttcatgag tttccagaca ttcgattttc atccccacat cgcggccggc
     3481 gtagaagccg cgggctacgt aaaccctacc gcaatccaga gtcaggcgat tccaccggtc
     3541 cttgcaggac gggatgtcat gggactggcc cagacaggaa ccggcaaaac cgccgccttt
     3601 gccctgccga ttctccatcg actgatgaag ggcgatcgtg gcaaggtccg tgcgctggtc
     3661 gtcgctccca cccgtgaact ggcggaacag attcaccaat ccatcgaaaa ccttggccgg
     3721 aagacccgcc tccggagcgt gacggtttac gggggcgtca agataaatcc ccagacggag
     3781 aagctcaagc gcggggctga gattgtcgtg
//
LOCUS       DAOSDR010000312         3808 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrimonas sp. isolate AMR_MDS_1826
            NODE_8466_length_3808_cov_3.582201, whole genome shotgun sequence.
ACCESSION   DAOSDR010000312 DAOSDR010000000
VERSION     DAOSDR010000312.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863355
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrimonas sp. (wastewater metagenome)
  ORGANISM  Petrimonas sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Dysgonomonadaceae; Petrimonas.
REFERENCE   1  (bases 1 to 3808)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3808)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:10:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,720
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 35
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3808
                     /organism="Petrimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8466_length_3808_cov_3.582201"
                     /isolate="AMR_MDS_1826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2023866"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(6..881)
                     /locus_tag="PLI30_11015"
                     /pseudo
     CDS             complement(6..881)
                     /locus_tag="PLI30_11015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009017319.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="carboxynorspermidine decarboxylase"
     gene            complement(976..3564)
                     /gene="clpB"
                     /locus_tag="PLI30_11020"
     CDS             complement(976..3564)
                     /gene="clpB"
                     /locus_tag="PLI30_11020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011966358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HOI80121.1"
                     /translation="MNFNNFTIKAQEAVQKAIDLTQASNQQMIEPAHLLKGVMLVGEN
                     VTHFLFQKLGVNVRNLEATVDKEIESYPRVSGGDPMLSRETNAVFQKSTDFANKMGDQ
                     FVSLEHLLLAIISEKNTASQLMKNAGISQNTLQSAINELRKGEKVTSQSAEDTYQSLG
                     KYAVNLNDRARNGKLDPVIGRDEEIRRVLQILSRRTKNNPILIGEPGTGKTAIAEGIA
                     HRIVRGDVPENLKSKEIYSLDMGALIAGAKYKGEFEERLKSVVNEVVKAEGEIILFID
                     EIHTLVGAGKSDGAMDAANILKPALARGELRAIGATTLDEYQKYFEKDKALERRFQTV
                     VVDEPTESDSISILRGLKEKYENHHKVRIKDEAIIAAVQLSHRYITDRFLPDKAIDLM
                     DEAAAKLRMEVDSVPYELDEIMRRVKQLEIEREAIRRENDTPKEEMLTRQIENLREEE
                     NVLKARWQSEKELINRIQQNKIDIEDAKFQAEKAEREGDYGKVAELRYGKIKEKEAEI
                     EQLKNQLHETQGGSAMIKEEVDAEDIADVVSRWTGIPVSKMLQSEREKLLHLEEELHK
                     RVVGQEEAITAVADAVRRNRAGLSDPKRPIGSFIFLGTTGVGKTELAKALADYLFDDE
                     NMMTRIDMSEYQEKFSATRLIGAPPGYVGYDEGGQLTEAIRRKPYSVVLFDEIEKAHP
                     DVFNILLQVLDDGRLTDNKGRVVNFKNTIIIMTSNMGSNVIRENFEKITERNREEIVE
                     NTKNLVMNMLKQTIRPEFLNRIDDIIMFAPLKQEEIAQIVRIQLNSVKKMLAENGVAL
                     EYTDEAVRSISEAGYDPEFGARPVKRVIQRKVLNQLSKDILSGKVDNSRPIVVDAIDE
                     NVYFRN"
BASE COUNT          850 a         1029 c          829 g         1100 t
ORIGIN      
        1 tgttacgcac cacggatccg cggtttgtag ggcatctcta agcagtcggg catatgacag
       61 gagaaagaaa cgttaagcat agcggtcagg acaccttcgt tttccactat atcggcaacg
      121 tcggccacca agactcccgt ctgccacgca aacgcgctgc cgggctccat aatgatctcc
      181 aggttgggat attttttctt aaaatggata agcaacccga ttaaatgttc cacatcgtaa
      241 tcttcgtgtg tcatcagatg gcctccgccc aagttcagcc actccacctt tttcagaaaa
      301 tgcccaaact tccgttctac agcctccaat gttttttcca ggtcgtatga attattttca
      361 cacaagttgt gcaggtgtag cccggaaact ccttccggca gggtatcacc catatttttt
      421 gccgtaaccc ccaaccgtga acccggcatg ctgggattgt agagatcggt ctccaccacc
      481 gaaaattcgg gattgatgcg gatcccgcat ttaattgcac cggcagaagc aactacctgc
      541 gggtaaaacc gatgaaactg agacaatgag ttgaaagtga tgtgactgct atatttcaga
      601 aacagcggaa aatcacaatc ggtatacgac ggtgcataag tatgcgcagg actgcccatt
      661 tcctcgaagg ccagccgtgc ttcggctatc gagcttgctg tggaatggcc aatgtattcc
      721 cgtacaatgg gaaatgcttt ccacatggca aacgctttaa atgccaatat aatattaact
      781 cctgccctct cttctaccga ttttatccgt tgcaaatttt tgcggagcaa ccgctcttcc
      841 attacatagc acggagaagg aattttactg aggtcgatca tatttatttt tttcgtaacg
      901 caaagctaca caaaaaatcg atgaacagga gtctgcatat aaaaaacaaa cgatcaaaat
      961 tgagatggca gcgggctagt tcctgaaata cacgttttca tcaatagcgt caaccacaat
     1021 cggcctggag ttatccacct taccggaaag gatatctttc gacaactgat tgagcacttt
     1081 acgctgaata acccgtttca cgggacgcgc accaaactcg ggatcgtatc ccgcttccga
     1141 tatgctccgt actgcctcgt cggtatattc cagtgcgaca ccgttctccg ccagcatttt
     1201 ctttacgctg ttcagctgaa tacgtacgat ttgtgctatt tcctcctgtt tgagcggggc
     1261 aaacataata atatcgtcta tacggttcag gaattcgggg cgaatggttt gcttcagcat
     1321 attcatcacc aggttcttgg tgttttcgac aatctcttcc cgattacgtt cggtgatttt
     1381 ctcaaaattc tcccggataa cgttcgaccc catgttggaa gtcatgatga tgatcgtatt
     1441 cttgaagttc accaccctcc ccttgttgtc ggtcagccgg ccatcgtcca gcacctgcag
     1501 caggatattg aaaacatcgg gatgtgcttt ctcaatttca tcaaacagca ccacggaata
     1561 gggttttcgg cggatggctt cggtcaactg ccccccttca tcgtaaccca cgtatccggg
     1621 aggcgccccg atcagccgtg ttgcgctgaa tttctcctga tattcgctca tgtcgatgcg
     1681 ggtcatcatg ttttcatcgt cgaacagata atcggccagg gcttttgcca gctcagtttt
     1741 tcccactccg gtcgttccca ggaagataaa cgaaccgatc gggcgtttcg ggtcgctcag
     1801 tccggcacga ttacggcgta cggcatcagc aacggcggta atggcttctt cttgtccgac
     1861 aacacgttta tgcagctctt cctccaggtg gagcagtttc tcgcgttcgc tttgcaacat
     1921 tttgctcacg ggaattccgg tccaccgtga aaccacgtct gcaatatctt cagcatccac
     1981 ttcttcttta atcatggcgc tgccaccctg tgtttcgtgc aactgatttt tcagttgctc
     2041 gatttccgcc tccttttcct ttattttccc gtatcgcaat tcggccactt ttccgtagtc
     2101 accttcccgt tcggcttttt ctgcctggaa tttagcatct tcaatgtcaa tcttgttctg
     2161 ctgtatccgg ttgatcagct ccttctccga ttgccaccgc gccttcagca cattttcctc
     2221 ttcgcgcagg ttctcgatct gcctggtcag catttcttcc ttgggtgtat cattctcgcg
     2281 acggatagct tcacgttcaa tttccaactg cttaacgcga cgcataatct cgtccagttc
     2341 ataaggtact gaatccacct ccatgcgtaa ttttgccgct gcctcgtcca tcaggtcaat
     2401 ggccttgtcg ggcaggaacc ggtcggtgat gtatctgtgc gacagttgca cggcagcaat
     2461 gatcgcttcg tccttgatac gcaccttgtg gtggttttca tacttttctt tcaatccccg
     2521 caggatggaa atactgtccg attccgtggg ctcatccacc acaaccgttt ggaagcgtcg
     2581 ttctagcgcc ttgtccttct caaaatactt ctgatattca tccagcgtgg tagcaccgat
     2641 agcgcgcaac tcaccccgtg ccaacgcagg tttcagtatg tttgcagcat ccatggctcc
     2701 atcgcttttt ccggcaccca caagcgtgtg gatttcatcg ataaaaagaa taatctctcc
     2761 ctctgccttc accacctcgt tgaccaccga tttcaaccgt tcctcaaact ctcctttata
     2821 ttttgccccg gcaatgagcg cacccatatc gagggaataa atctctttgc tcttgaggtt
     2881 ctcgggtaca tctccacgaa cgatacggtg tgcgattcct tcggcaatgg ctgtttttcc
     2941 ggttcccggc tcaccgatga gaatggggtt atttttagta cgcctgctca ggatctggag
     3001 cacgcggcga atctcctcat cgcggccaat aacggggtcg agcttgccgt ttctggccct
     3061 gtcgttcaga ttcacggcat attttcccag cgattgatag gtatcttccg cagattggct
     3121 ggtcactttt tcacctttgc gcaactcgtt aatggccgat tggagcgtgt tctgtgaaat
     3181 tccggcattt ttcatcaact gcgaagcagt gttcttctcg ctgatgattg ccagcagtaa
     3241 atgctcgagc gacacgaact ggtcgcccat tttgttcgca aaatcggtag atttctgaaa
     3301 aaccgcattg gtttcacggc tcagcatcgg atctccgcct gaaacgcgtg ggtacgattc
     3361 aatctcctta tccacagttg cctccaagtt tctcacattc actcccaact tctgaaacag
     3421 aaaatgggta acgttttcgc ccaccagcat cacacctttg agcaggtgag cgggctcaat
     3481 catctgctga ttgcttgcct gcgtcagatc aatggccttc tgaacggctt cctgggcttt
     3541 gattgtaaaa ttgttaaagt tcatatgttg tttttttaga ttttcaaacc tcggcgccaa
     3601 gaatcaagaa agacgaaaag agactgtcag tcgggtagat ctttttgtta aaagtcttgt
     3661 ttcttgcatc ttggcgttct acttttcagg tgcaattttc ttgccaaaga gaaatttatg
     3721 acgatttggc agggactacc ttctccaact gttccacggc ttctcccaac cggttaaact
     3781 cgtgcggata cccgttcgga tggttgcc
//
LOCUS       DAOSDR010000313         3782 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrimonas sp. isolate AMR_MDS_1826
            NODE_8544_length_3782_cov_3.995170, whole genome shotgun sequence.
ACCESSION   DAOSDR010000313 DAOSDR010000000
VERSION     DAOSDR010000313.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863355
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrimonas sp. (wastewater metagenome)
  ORGANISM  Petrimonas sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Dysgonomonadaceae; Petrimonas.
REFERENCE   1  (bases 1 to 3782)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3782)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:10:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,720
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 35
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3782
                     /organism="Petrimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8544_length_3782_cov_3.995170"
                     /isolate="AMR_MDS_1826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2023866"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2190)
                     /locus_tag="PLI30_11025"
     CDS             complement(<1..2190)
                     /locus_tag="PLI30_11025"
                     /inference="COORDINATES: protein motif:HMM:TIGR01451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI80122.1"
                     /translation="MKKIYILKKAAGVVGYFTFILTLIAGNFDQSEAQVSVPFAERTS
                     VYTPTQTIYDIKGDFTMIGNTNMTLANYSDGGNNSSDMRYVDIDGDLNTLNSSSATLQ
                     FSTENGAIPECSNIIYAGLYWTGRAHNGTSPNTFSVTKEVPGAGTPQTINNNQTVSGG
                     QTITYTNYSLTVTRSGNTNNRTITYTFNTSAVGDKVAFIYAHNSGSPTLMVSVNDGPA
                     TAVSTSSIDASNAYLATPYIIFSETGGITFTVNRFYRNGSNSQSDDARAYTNISGTYR
                     PPLSVTKNFDKGVVYLKHVSAAGYTQINAADANFTKEIYYPTSSDGYMYSAYAEVTDY
                     VRQYGLGEYFVADIALQEGDGGNTGFYGGWGMIVIYENSKMSWRNVVVFDGHAYVQGN
                     TTINYELPVAGFSTVQSGEVNMKLGLMAGEGDRSISGDYFNIRNHSNTTWVDLSHSGN
                     NSTNFFNSSINSGSNPRNPSILNNTGVDIVMFDIPNPGNTVITNNQTSTRFRYGSTQD
                     TYVIFCLAMAVDAYVPDLEAHNQTISIEGVPVPPNTPIDVEPGQEIEYSIEIKNKGTE
                     PINNAVVSIPIPYTTSYISSSAVYYEGLTGPQPVFNPGSGATGSILWNIGNIPLPANS
                     ETLLAKMTYKVRVTTDCFILSNPNCDPLVTINGGISGTGATSGVSFEGRSFIQGYQTN
                     GVCTGEPITDPVAISIDRENYIATNCTGEDYTTRAFTYCNTIGSTIPF"
     gene            complement(2217..2789)
                     /locus_tag="PLI30_11030"
     CDS             complement(2217..2789)
                     /locus_tag="PLI30_11030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI80123.1"
                     /translation="MKKHLFCDRSLTYRKSISFVTLWLLVGLYAFSTANVFGQEVRQL
                     DNFLSTHSSSTEKEQQEKANLEQLLLGLRPTLYLQSGELIQPGQSAALRITTDVQSAK
                     LLNEENPLFSKVEVVRINIDSQGDLTFSFNLDNLPSLENLKYVVFRCSFDICEGQPEK
                     NACEVKKISTMVHSASGKAITILYQIISPS"
     gene            3576..>3782
                     /locus_tag="PLI30_11035"
     CDS             3576..>3782
                     /locus_tag="PLI30_11035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI80124.1"
                     /translation="MKHSFVLFWNVDTQNDFVEPWGKLYVPGAELLKPVWAQITGLAQ
                     RQEIRVVNTADHHYIYSAEIDEHPD"
BASE COUNT         1033 a          735 c          847 g         1167 t
ORIGIN      
        1 aaatggaatg gtggagccga tggtattaca gtaggtaaaa gcacgggtgg tataatcctc
       61 accagtacag ttggttgcga tataattttc cctgtcaatc gaaattgcta ccggatcggt
      121 aataggttcc cccgtacaga ctccatttgt ttggtaacct tggataaaag aacgcccttc
      181 aaaagagaca ccagaggtgg cgcctgtgcc actgatgcca ccgttgatgg tgaccaacgg
      241 gtcacaattg ggattagaaa ggataaaaca atctgtagtg actcttactt tataggtcat
      301 tttcgcaagc agggtttcac tgtttgcagg caaaggaata tttccaatat tccataaaat
      361 agatccggtt gccccggatc ccgggttaaa tacgggctgt ggcccagtca aaccttcata
      421 atatacagca gaactactta tataggatgt agtataagga atggggattg aaacgactgc
      481 attgtttatg ggttcagtcc ctttgttttt gatttcgata ctgtattcaa tttcctgccc
      541 cggctctaca tcaatagggg tattaggagg tacgggcaca ccctctatag aaatggtctg
      601 gttgtgagct tccagatcgg gtacgtaggc atcaacagcc atggctaaac agaatatcac
      661 ataagtgtcc tgtgtggagc catatctgaa acgggtagat gtttggttat tggtaataac
      721 cgtattcccg gggtttggga tatcaaacat tacaatgtca actcccgtat tatttaagat
      781 actgggattt cgaggattgg aaccggagtt aatcgagctg ttgaaaaagt tcgttgaatt
      841 attgcccgaa tggcttaagt caacccaggt ggtattagaa tggtttcgga tattaaaata
      901 atcgccggat atactccggt cgccttctcc ggccatcaac ccaagtttca tgtttacttc
      961 cccactctgg acagtagaaa agccggcaac cggtaattcg taatttattg ttgtatttcc
     1021 ctgtacataa gcatgtccgt caaaaacaac aacgttccgc cagctcattt tggagttttc
     1081 ataaattacg atcatacccc atccgccata aaaaccggta ttgcctccat ccccttcttg
     1141 cagagctatg tcggccacaa aatattcgcc cagcccatac tggcgcacat aatcggtaac
     1201 ttctgcatag gccgaataca tataaccatc agaagaagta gggtaataaa tttcctttgt
     1261 gaaatttgca tctgcggcgt ttatttgggt gtaacccgct gcgctaacat gttttaggta
     1321 aaccacgcct ttgtcaaaat ttttggtaac ggatagcggt gggcggtagg tgcctgaaat
     1381 attggtatag gcccttgcgt catcactctg agaattgctc ccgtttctgt aaaaacggtt
     1441 tacagtaaaa gttatccccc ctgtttcgct aaaaataatg taaggggtgg ccagatatgc
     1501 gttagacgca tcaatagagg aagtggagac cgctgtggca ggcccgtcat taacgcttac
     1561 cattaaggtc gggctccctg aattatgagc ataaataaat gcaactttat ctcccacagc
     1621 ggaggtgtta aacgtgtacg taatggtcct gttgttggta tttccacttc ttgtaaccgt
     1681 caaagaataa ttagtgtagg ttatggtctg tccacccgat actgtctgat tattatttat
     1741 ggtttgcggg gtgcctgcgc caggtacttc tttagtgacg ctgaacgtat tggggctggt
     1801 cccattgtgg gcccgtcccg tccagtataa tccagcataa ataatgttgg aacattccgg
     1861 tatagcgcca ttttcagttg aaaactgtag ggtggccgaa gaggaattta aagtattaag
     1921 gtctccgtca atatctacat acctcatgtc gctgctgttg tttccaccgt cggaataatt
     1981 agcaagcgtc atattggtgt tcccgatcat ggtaaaatca cctttgatgt cgtagatagt
     2041 ttgagtaggg gtgtataccg aggtacgctc ggcaaaaggc acactcacct gagcttcaga
     2101 ttggtcaaaa ttaccggcaa tcagcgtaag gatgaaagtg aaataaccaa ctacccctgc
     2161 tgcttttttt aggatgtaaa tttttttcat gtttgttttt tttgtcggtt aagggattaa
     2221 gaaggagata tgatttgata caaaatggtg attgcctttc cggaggccga atgaaccatt
     2281 gttgatatct ttttaacttc gcaggcgttt ttctcaggtt gtccttcaca gatatcgaat
     2341 gaacatctga atacgacgta tttcaggttt tccagagaag gtagattgtc caagttgaag
     2401 gagaacgtta agtccccctg cgagtcaatg ttgatcctca ctacttcaac ttttgaaaac
     2461 aaagggtttt cttcattaag taatttcgca gattgaacat cggtggttat tcggagtgca
     2521 gccgattggc ctggttgtat taactctccg ctttgcagat aaagggtcgg tcttagaccc
     2581 aatagcaact gttcgagatt tgctttttct tgttgttcct tctcagttga agaactatgt
     2641 gtagacagaa aattatctaa ttgccgtacc tcctgtccaa acacgtttgc cgttgaaaaa
     2701 gcgtacaatc caacaagtag ccacaaggtt acaaaactta tactttttct gtaagtcaat
     2761 gatctgtcgc aaaataaatg ttttttcata gtcccttaat tttacttgga ttgttaatga
     2821 tgaattggat aaatatttat tagatggtta ttgtgagtat ttgtaaaaag taagttgacc
     2881 cccttactga agaggttttt agcggtttat tctgagaagg gaaaatgctc ggaatagccg
     2941 gatggcatga attttcagat gtagctgtta tgcccctttt tgtcataata atacttgttg
     3001 cagggagagg gggcagccaa cgcttctgta acgaaaacgt tatattgccc ttatccctta
     3061 atttgcattt ttttaatccc atagctaatt tagtgctttt tggtatctaa ctccaaattt
     3121 tttttagatt ctttgaggcc agtgggaaaa acctttcaga tcatcgcttt tgcaggtctt
     3181 ttgattgcta ttttctgaaa agtttgtaac ccgcgttaag atcaaaaccg caatttttaa
     3241 attccggaat agattttggc aaaaataatg ccgtgtcgtg taagcttctc tttgttagat
     3301 atttgtattt ttgtatagag taagtggaac tcccgaaaaa gaaacgtcat tcccaaattt
     3361 agaatcattc tggataattg ttattgaaaa atagaaggag tgaatatatt gttggtaaag
     3421 tttgggaaag aactgttaag gctcgttttt agaattcccg cggcagatcc tgtttgttat
     3481 caatccggag gagagctttc agataaacgg catttgtggg ctgtttttga ccagtttggt
     3541 gaataaactt ctaaatttga agaaaaaatc cggctatgaa acattcattt gtgctgtttt
     3601 ggaatgttga cacccaaaat gattttgtgg agccctgggg aaaactgtat gtgccgggcg
     3661 ctgaattgct gaaaccggtt tgggcccaaa taaccggatt ggcccagcgt caggaaatcc
     3721 gggtggtaaa tacggccgac catcattaca tctattccgc agagatcgat gaacatcccg
     3781 at
//
LOCUS       DAOSDR010000314         3773 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrimonas sp. isolate AMR_MDS_1826
            NODE_8578_length_3773_cov_6.590909, whole genome shotgun sequence.
ACCESSION   DAOSDR010000314 DAOSDR010000000
VERSION     DAOSDR010000314.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863355
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrimonas sp. (wastewater metagenome)
  ORGANISM  Petrimonas sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Dysgonomonadaceae; Petrimonas.
REFERENCE   1  (bases 1 to 3773)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3773)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:10:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,720
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 35
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3773
                     /organism="Petrimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8578_length_3773_cov_6.590909"
                     /isolate="AMR_MDS_1826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2023866"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            410..2086
                     /locus_tag="PLI30_11040"
     CDS             410..2086
                     /locus_tag="PLI30_11040"
                     /inference="COORDINATES: protein motif:HMM:NF012234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOI80125.1"
                     /translation="MDNITTQAQERGKIPSLQPDNLLKNIEQIIELSENSELSDEFFD
                     QAKPYINFVKDKLQLTDMAALFFAVFVDNNDTGYSSVKKLSNHFKCRKIHIMRYGDSF
                     LELERRRLIRCKRSDNEIGYRIPAEVIYALHDGREIEPREISDLSQDDFFSRVNELFT
                     ERKESEIRYDHLVIEMNELVNANLHLNVCKIIKKHRYTDIEEILLLYICHALVNNDDD
                     FVSFFEMQLIFEFKDLSAIKKSLKRGNNSLIEDLWIENIRSEGFSDVNHYQLTDKAKR
                     ELIGSNLLQKDAIEDGDNFIMHGTIVKKDLFFDTQVQNQIEQLTNLLIPENFAAVKRR
                     LSSNGMRNGFSCLFYGPPGTGKTECVYQIARKTGRNILTVDYSEIKSKWVGESEKSIK
                     RIFDIYRECVNSSETAPIFLFNEADALLGTRLETLRSVDKMENALQNIILQEMENLNG
                     IMIATTNLTRNFDEAFERRFIYKIKFLNPHWDAKRKIWQAMLQSLPDAHAEELAARFD
                     FSGGQIENIVRKITVELVLTGKDLSLDMIREFCRNEQFNHFDRRPRIGFN"
     gene            2109..3047
                     /locus_tag="PLI30_11045"
     CDS             2109..3047
                     /locus_tag="PLI30_11045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004315852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WYL domain-containing protein"
                     /protein_id="HOI80126.1"
                     /translation="MATSRKNNKERTSTLFNRYVWLVETILREGRITFEEINDKWNRS
                     RLNYSGDDIPLRTFHNHRQAIEEMFDINIECDKRNGYVYFIENADDIHRNGMRSWMMN
                     AFYVGNLLNESREMKDRILFEKIPSGHQFLTSFLEAIRDNLTVEITYQSFEKDVPYTF
                     PVEPYCLKVFRQRWYVLARTPHYNELRVYSLDRLHQVVITNTPFIYPVDFSPENYFHH
                     NFGIIHSGSEVPDTVVVKVYDNQRRYFETLPLHHSQTLVEDQIEYALFRYLLHPTYDF
                     VQELLSHGENVEVLKPIWLRQEIADVVQQMSVMYKS"
     gene            3138..3632
                     /locus_tag="PLI30_11050"
     CDS             3138..3632
                     /locus_tag="PLI30_11050"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007661946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HOI80127.1"
                     /translation="MKNFAAIDFETANSQLTSVCSVGVVIVRDGVITERFYSLIRPEP
                     EYYSYWNKRVHGITYDDTVNAPIFPQVWRQIEPLVEGLPLVAHSKRFDENCLKACFRT
                     YQMDYPDYEFHCTLQGARSQLKGLPDYHLDTVSAHCGFFLENHHHALADAEACARIAI
                     EIFK"
BASE COUNT         1151 a          776 c          789 g         1057 t
ORIGIN      
        1 ccacttttcg taattctttc ctgaagcgga attgccaaat taactaccta tctgaaccga
       61 ctctttcaat tgttcatatt tttcaacagt tctataattc taatagaaat actaaatttg
      121 aattagtaat tgcttaataa acttcattta tcattttatt ttcttgctga tcatattttt
      181 ttctttgctt gtcaatcccc actcctccgc aaagatactg ttgctttcac tataatattg
      241 caaggtcaaa ccttccggat ttcacttatt tcttctaccc ttcaatgtaa ggtacatgta
      301 cacacattac cggatgtttt cagaaatttt tacaaatatt tatatgatgt gccattattc
      361 tgcataactt taccctacat ttgcattgta acgataatga aacagggcaa tggacaatat
      421 cacaacacaa gcacaagagc ggggtaagat tccctctctt caaccagaca acttgttgaa
      481 aaacattgag cagattattg agttgtcgga aaattccgag ttgagtgatg agttttttga
      541 tcaagcgaaa ccttacatca attttgtaaa ggataaattg caactgactg atatggcagc
      601 cctttttttt gctgtttttg tggataataa cgataccggt tactcatcag tcaaaaagtt
      661 gtccaatcat tttaaatgcc gtaagatcca tatcatgcgc tacggggatt cgttccttga
      721 gctcgaaagg agacgcctta tccgttgcaa aagaagtgac aatgaaatcg gttaccgtat
      781 tccggctgaa gttatctatg cattacatga tggcagagag atcgagccaa gagagatttc
      841 tgacctgtcg caggatgatt ttttcagtcg ggtgaatgaa ctttttacag aaagaaaaga
      901 atctgagatc cgttatgacc atctggtaat tgaaatgaat gagttggtga atgccaatct
      961 tcatcttaac gtatgcaaaa tcatcaagaa gcatcgctat acggacatag aggagatcct
     1021 gctgttgtat atatgccatg cgttggtgaa taatgacgat gattttgtca gttttttcga
     1081 aatgcaacta atttttgaat ttaaagactt atcagctatt aaaaagtcat taaaaagggg
     1141 aaacaatagc ctgattgaag atctgtggat tgaaaatatt cgtagcgaag gtttctccga
     1201 tgtaaatcat tatcaactga cagataaagc caagcgggag ttgatcggaa gcaatctttt
     1261 acaaaaggat gcaattgaag atggtgataa ttttatcatg catggtacga tagttaaaaa
     1321 agaccttttt ttcgacacgc aggtgcagaa tcagattgaa cagttgacca atctgctgat
     1381 acctgagaat tttgcagcgg taaagagaag actttccagc aacggaatgc gaaacggttt
     1441 ttcctgcctc ttttacggtc cacccggtac cggtaaaact gaatgtgtct accagatagc
     1501 acgcaaaaca ggtcgcaata tcctgacagt cgactattca gagataaaaa gcaagtgggt
     1561 tggagaatca gaaaagagta ttaaaagaat tttcgatatt tatcgagaat gtgtaaatag
     1621 ttctgaaaca gctcccatct ttctgttcaa cgaagcggat gccctgctcg ggacccgact
     1681 ggagacactg cgttcggtag acaagatgga aaacgcactg cagaacatca ttctgcagga
     1741 gatggagaac ctgaatggga tcatgattgc cactaccaac ctgaccagaa atttcgatga
     1801 ggctttcgaa agaaggttta tctacaaaat taaattttta aatccgcatt gggatgcaaa
     1861 acgaaagatt tggcaagcga tgcttcagag ccttcccgat gcacatgcag aagaattggc
     1921 tgcacggttt gatttctccg gagggcaaat agagaatatc gtcagaaaaa tcactgttga
     1981 actggttctt accggtaaag acctttcgct tgatatgatt cgggaattct gtcggaacga
     2041 acagtttaat catttcgatc gccgacccag aatcggattt aattagcatg taacaagaag
     2101 taatataaat ggcaaccagc agaaaaaata acaaagagcg tacctccacc ctgtttaaca
     2161 gatatgtctg gttggtggag accattctaa gagaaggtag aattaccttt gaagagataa
     2221 acgacaagtg gaaccgttcc cgcctgaact actcgggaga tgacattccc ctgcgtacct
     2281 tccacaatca ccgccaggcc atcgaggaga tgttcgacat caacatcgag tgtgacaagc
     2341 ggaatggata cgtctatttc atcgaaaacg cggatgacat ccatcgaaac ggcatgcgat
     2401 cctggatgat gaacgctttc tatgttggaa atctgctcaa tgagagccgc gagatgaaag
     2461 accggatcct gttcgaaaag attccttccg gtcaccagtt cctgacatca tttctcgaag
     2521 ccatccggga caaccttacc gttgagatta cctaccagag ctttgagaaa gatgtacctt
     2581 acacctttcc ggttgaaccc tattgtctga aagtgttccg gcagcggtgg tacgtgctgg
     2641 cccgaactcc tcattacaac gagttacgcg tttactcatt agatcgcttg catcaagtgg
     2701 taatcactaa cactccattc atatatccgg tagatttctc accggaaaat tattttcacc
     2761 acaactttgg cattatacac agcggaagtg aagttcctga tacggttgtg gtcaaagtgt
     2821 acgataacca gcggcgatat tttgagacgc tacccctgca tcattcgcaa acgttggtgg
     2881 aagatcagat tgagtatgcc ctctttcgat atctgctgca tcccacctac gattttgtgc
     2941 aggaattgtt atcgcacggc gaaaatgtag aagtgttgaa accaatttgg ttgagacagg
     3001 aaatcgctga tgttgtacag caaatgtcgg taatgtataa aagttaagaa attaatattt
     3061 ttcttttcaa tgttgaagtg aataagaaaa gaaatattta catttgaact attaaaatga
     3121 aaaacaattt gttgagcatg aaaaactttg ccgctatcga ttttgaaaca gccaactcac
     3181 aacttacgag cgtttgcagc gtgggtgtgg taattgtccg cgacggagtg atcacagaga
     3241 ggttttacag cctgatccgc cccgaaccgg agtattacag ttattggaac aagcgggtgc
     3301 atggcatcac ctacgatgat accgtgaatg ccccaatctt tccgcaagta tggcgtcaaa
     3361 ttgaaccact cgttgaaggt ctgccgctgg ttgcgcatag caagcgtttc gacgagaatt
     3421 gtctgaaagc ttgtttccgt acctaccaga tggattaccc cgattatgag ttccattgta
     3481 ccctgcaggg tgcgagatca caactgaaag ggttacccga ctaccatctt gacactgtat
     3541 ccgctcattg tggtttcttc ctcgagaacc atcaccatgc cctggccgat gcagaagcgt
     3601 gtgcgagaat agcgattgag atattcaagt aaaaataact gattttttta ccatgtcaaa
     3661 agtattcaaa gtaacaccaa aacgcatcaa acgtaccaac ggtactgtgt taaccccgca
     3721 gatgacagtg actgtaacca cgctacagca cacccacgat ccattctaca acg
//
LOCUS       DAOSDR010000315         3769 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrimonas sp. isolate AMR_MDS_1826
            NODE_8589_length_3769_cov_4.054658, whole genome shotgun sequence.
ACCESSION   DAOSDR010000315 DAOSDR010000000
VERSION     DAOSDR010000315.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863355
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrimonas sp. (wastewater metagenome)
  ORGANISM  Petrimonas sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Dysgonomonadaceae; Petrimonas.
REFERENCE   1  (bases 1 to 3769)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3769)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:10:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,720
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 35
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3769
                     /organism="Petrimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8589_length_3769_cov_4.054658"
                     /isolate="AMR_MDS_1826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2023866"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            103..486
                     /locus_tag="PLI30_11055"
     CDS             103..486
                     /locus_tag="PLI30_11055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013617307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI80128.1"
                     /translation="METRINKLSEIDRRNPFTVPEDYFSRFNEHIMARLPEKEMVEPK
                     KVSMWDKAKPWVYMAAMFMGIFFTIQLLTRNTTNQVATISSTQFTDDYWSTVQITEEE
                     FYQYLEEQLVNDGYYDYMYNQVYMN"
     gene            559..1005
                     /locus_tag="PLI30_11060"
     CDS             559..1005
                     /locus_tag="PLI30_11060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI80129.1"
                     /translation="MRKSITLLCFTLIVSAFTFASFAQVRKMNMADYEKRKMEYIQKE
                     AGLTKEEANRYFPLYNDLSKKKFELHKQHRDKVEKMKQRNKNMSNEEYRQLLENDVDV
                     KLKEAELDKQYSEKLEKILSPEKLYRAQQAERKFMQREVMKFRGSE"
     gene            1281..2477
                     /locus_tag="PLI30_11065"
     CDS             1281..2477
                     /locus_tag="PLI30_11065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005837410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HOI80130.1"
                     /translation="MPQISERGLQMPASPIRKLAPLSDAAKARGVKVYHLNIGQPDLP
                     SPQVALDAIRSIDRTTLEYSPSDGFRFFRENLVNYYAKFNIHLLPEEIIVTAGGSEAV
                     LYAFMACLNPGDEIIVPEPAYANYMAFAVSAGAIIKTIPTSIDTGFKLPEIEKFEALI
                     HSKTKGILICNPNNPTGYVYTAEEMEQIRLLVKKHNLYLFSDEVYREFIYSDAPYISA
                     CHLKDIEENVVLIDSVSKRYSECGIRIGCLITKNKKLHDTVMKFCQARLSPPLIGQIA
                     ANASLQENGAYMQQNFDEYKSRRDFLIERLNKIPGVYSPQPEGAFYTLASLPVDDTDA
                     FCAWCLSDFRYENQTIFMAPASGFYVTPGLGKNEVRIAYVLNKEDLNNALIVLEKALE
                     AYNNKQ"
     gene            2500..3747
                     /locus_tag="PLI30_11070"
     CDS             2500..3747
                     /locus_tag="PLI30_11070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005864454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOI80131.1"
                     /translation="MNTELFIAKRIYRGDKKNEKRVSSPAIKIAISGIALGLAIMLAA
                     VCIIIGFKKEIREKVIGFGSHIQITSFDSNSSYEHHPISFSDTLKNRLLSNPKIARVQ
                     KFITKPGIIKTDEDFLGIVLKGISDDYDWQFFKKNLIEGAIINPNDTSGINRAIISQN
                     IANKLHLKLGDHFTTYFVQEPVRARRFTVSGIYETNFEDYDKLYVLTEAKILSGLNDW
                     EDDMVSGVEVLVKEYNQLDQTAQDLFFEMSNYQDRFGNRLYTRSIKDINPMIFNWLNL
                     LDMNVWVILILMILVSGFTMISGLLIIILERTNMIGMLKSMGARDFSIRKVFLYLSAF
                     LIGQGMLWGNVMGLTFCILQKEFNILRLDSATYYLSAVPIDLNPWYVILLNVGTLIVS
                     LIMMIAPSYLVAKITPAKSIRFE"
BASE COUNT         1215 a          775 c          766 g         1013 t
ORIGIN      
        1 atcatgccgt taaaaagatt gaaaattttt taacagattc caattaaacc tttatatagg
       61 gagagagtct actcatcgta gtacagcgca aaaaaaaatg gtatggaaac aagaataaat
      121 aaattgagcg agatcgacag aagaaatcca tttactgtac ccgaggatta tttttcgagg
      181 tttaacgaac acataatggc tcgtttaccc gagaaagaga tggtagaacc aaaaaaggtt
      241 tccatgtggg acaaagccaa accctgggtt tatatggctg ctatgtttat gggtatcttt
      301 tttactatcc aattgctaac aagaaacacc acaaatcagg tcgctacaat atcatctacg
      361 cagtttaccg atgattactg gtcgaccgtg cagataacag aagaggaatt ttatcaatat
      421 ttagaagaac aattggtgaa tgacggttac tacgattata tgtacaacca ggtttatatg
      481 aactaagcaa tgagagcggg caatttcgct tcgctcaatt gccatacggc tacaaagaaa
      541 aaaataaata acaattgtat gagaaagagc atcacacttc tttgttttac cctcatagtc
      601 agcgccttca cgttcgcatc ctttgcccag gtccgtaaaa tgaatatggc cgattacgaa
      661 aaaaggaaaa tggaatacat tcagaaagaa gcgggattaa ccaaagagga agctaaccga
      721 tattttccgc tgtataacga tttgtccaag aaaaaattcg aacttcacaa acaacaccgg
      781 gataaagtgg agaagatgaa acaacgcaat aaaaacatgt ctaacgaaga ataccggcag
      841 cttctggaaa acgatgtaga tgtaaaacta aaagaagccg agctggacaa gcaatattcc
      901 gaaaaactgg aaaaaatcct ctctcccgaa aaactctatc gggcacaaca agccgagcgg
      961 aaatttatgc aaagggaagt gatgaaattt agaggaagcg agtaaaaatt caacgagatt
     1021 aataacacct atttcccagg gattgtacag gcacacatcg actggactat ccgtacctgt
     1081 aggggtttcc ccgtaaagca cttgaacgaa ttttgttgac accgtattag aaaattagga
     1141 ctgatgagtc ataacatagt taatttgtaa aaaaaacggg attaaccgtt acaaaataaa
     1201 aggaatccat ccaattttat taactttgca ccttgtttag cggaaatcat tagatttttt
     1261 ccgtaattag ttgtaagaat atgcctcaga tttcagagcg gggcttacag atgcccgcct
     1321 ctcccattag aaaattagct ccactatcgg atgcagcaaa agctcgcgga gtaaaagtat
     1381 atcacctgaa tatcggtcag cccgatttgc catcgcctca ggtagccctg gacgctattc
     1441 gaagtatcga caggaccacg ttggaatata gtcccagtga cggatttcgt ttttttcgcg
     1501 aaaatctggt gaactattat gcaaaattca acatccatct tttacctgaa gaaattatcg
     1561 ttaccgcagg tggttcggaa gccgtgctgt atgcctttat ggcttgtcta aatccgggcg
     1621 atgaaatcat tgtccctgag ccggcatatg ccaattacat ggcttttgcc gtatctgcag
     1681 gtgctattat caaaaccatt ccaacgagta tcgataccgg atttaagctc ccggaaatag
     1741 aaaaattcga agcgctcatc cattcgaaaa ctaagggtat ccttatctgc aacccgaaca
     1801 atcccaccgg atatgtttat acagcagagg agatggagca aatcaggctg ttggtaaaaa
     1861 aacacaacct ttacctgttt tccgacgaag tataccgaga gtttatttat agcgacgcgc
     1921 cttacatctc ggcttgccac ttgaaggata ttgaagagaa tgtcgttctt atcgattctg
     1981 tatcgaaacg atacagtgag tgtggaattc gtataggatg cttgattacc aaaaataaaa
     2041 aactgcacga cacggtgatg aagttctgtc aggcacggtt gagccctccg ttgattggac
     2101 aaattgccgc aaatgcttcg ttacaggaaa acggagcgta catgcagcaa aattttgatg
     2161 aatacaaaag tcgccgtgat ttcctgatcg aacggctcaa caaaattccc ggtgtttatt
     2221 cgcctcagcc cgaaggcgcc ttctatacat tagcgagcct tcccgtggac gatacggatg
     2281 cattttgcgc atggtgcctt tcggatttcc gatacgaaaa ccaaaccatc tttatggctc
     2341 cggcatcggg attctacgtc actcccggat tagggaaaaa cgaagtccgc atagcttatg
     2401 tactcaacaa agaagattta aacaacgcgt tgattgtgtt ggaaaaagca ttggaagcct
     2461 ataataacaa acagtagtgc tcaacgctca aaacaaaaaa tgaacacaga gctttttatc
     2521 gccaaacgga tctacagagg tgacaagaaa aacgagaaac gtgtttcatc gcctgctata
     2581 aaaattgcta tctccggcat agcgttaggc ttagcaataa tgttagcagc tgtgtgcatt
     2641 attattggtt ttaaaaaaga aattcgtgaa aaggtgatcg gttttggttc tcatatccag
     2701 attaccagtt ttgacagcaa cagttcttac gaacatcatc ctatctcttt ttccgataca
     2761 ttaaaaaaca gactgttaag caatccaaaa attgcccggg tacaaaagtt cataactaaa
     2821 cccggtatta tcaaaaccga tgaagatttt ttaggtatcg tgctgaaagg gatatccgat
     2881 gattacgatt ggcaattttt taaaaaaaac ctgatcgaag gagccattat aaacccaaac
     2941 gacacatcgg gtatcaaccg ggccataatt tctcaaaaca ttgccaacaa acttcacctt
     3001 aaattaggcg atcattttac tacttacttt gttcaagagc ctgttcgtgc cagacgattt
     3061 accgtttcgg ggatttacga aaccaatttc gaggactacg acaaactgta cgtcctgacc
     3121 gaagcaaaaa tcctgtccgg attaaacgac tgggaagacg acatggtaag cggtgtggaa
     3181 gtattggtaa aagagtataa ccaacttgat caaacggctc aggatctgtt tttcgaaatg
     3241 tcaaactacc aagaccggtt tggcaacagg ctttacactc gctccataaa agacatcaat
     3301 cccatgatct ttaattggtt gaaccttctg gatatgaatg tttgggtaat cctgatattg
     3361 atgattctgg tgtctggttt tacgatgatc tcgggacttc tcatcatcat cctggagcgg
     3421 acaaatatga tcggcatgct caaatcgatg ggggcacgtg atttcagtat ccggaaagta
     3481 tttctttacc tttcggcttt tctcatcgga caaggaatgc tctggggtaa cgttatggga
     3541 ctaaccttct gtattttaca aaaagaattc aatatcctaa gactcgattc tgccacttac
     3601 tatctctctg ccgtacccat cgatctgaac ccttggtatg tgatcttgct aaatgtgggg
     3661 acactgattg tttccttgat aatgatgatt gctccctcct atcttgtggc aaaaattacg
     3721 cccgcaaaat caatccgttt cgaataaata accttactgg ctgattata
//
LOCUS       DAOSDR010000316         3765 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrimonas sp. isolate AMR_MDS_1826
            NODE_8599_length_3765_cov_4.201348, whole genome shotgun sequence.
ACCESSION   DAOSDR010000316 DAOSDR010000000
VERSION     DAOSDR010000316.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863355
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrimonas sp. (wastewater metagenome)
  ORGANISM  Petrimonas sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Dysgonomonadaceae; Petrimonas.
REFERENCE   1  (bases 1 to 3765)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3765)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:10:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,720
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 35
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3765
                     /organism="Petrimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8599_length_3765_cov_4.201348"
                     /isolate="AMR_MDS_1826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2023866"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(122..1144)
                     /locus_tag="PLI30_11075"
     CDS             complement(122..1144)
                     /locus_tag="PLI30_11075"
                     /inference="COORDINATES: protein motif:HMM:NF025465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PmoA family protein"
                     /protein_id="HOI80132.1"
                     /translation="MKKTVKALIIAALIMHVSCQGTSTNVRFNVSEEKKAVEVYVNDI
                     YFTSFIYPENLEKPCLYPIKTPSGKFITRGFPLNPRPFERTDHPHHIGLWFNFGDVNG
                     LDFWNNSYSIKPEDKDKYGIILFDKVIEADNRKGRLVVQSKWTDNNGSILLNEKTTYI
                     FEGLKNDIRTIERVTELTANKEVTFTGNKEGLIGLRVDRTFEEPTQKAEKFLDANGNV
                     TETPALNNEGVNGFYRNAEGLTRGDVWGKRSKWVALKADKEGETITIVIMDNKNNPNY
                     PAWSHARGYGLFAANNLGGKVVDKNSDPVELKLSSGEKVTFKHKVIIAGDMSDQEINR
                     FSDDFN"
     gene            complement(1260..2621)
                     /locus_tag="PLI30_11080"
     CDS             complement(1260..2621)
                     /locus_tag="PLI30_11080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019538156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOI80133.1"
                     /translation="MKNSRRDFIKKSALGVAGITLGSTMNMSAKSYANIIGSNDKVRV
                     GILGFSNRFRGALGKSFLKYAKEMNFELFTVCDIWNRRRDEGQAWYKEQAGGDLRTAR
                     NTDELWSQKPDAVIISTADFQHALHTAEAVRAGCDVYVEKPFAETMDDANFALKAAKE
                     TNKVIQVGSQRRSGTNYHAAYDYIKSGKFGKIIAVEMTWNVNQPGRWRLPQLTREIRE
                     QDTDWKRFLMNRPYEPWDPRKYLEYRLFWPYSSGIPGQWMAHQIDTVHWFSELDYPRS
                     VVANGGIYMWNDGRTNYDTMTAVFDYGHAKEKEPGEGFQVIYSSRQNNSSGGTKEWYF
                     SNGGKLDLDTNIINNDGGLTERHAGDMGMKPFMLNTFELPKISVETSSDTGSDPLTNA
                     HMKNWMDCVRAGNVKTNAPVEAGYNHSIADIMVTAALRTGQRAIFDEKQKQVMAGGKV
                     FKY"
     gene            complement(2660..>3765)
                     /locus_tag="PLI30_11085"
     CDS             complement(2660..>3765)
                     /locus_tag="PLI30_11085"
                     /inference="COORDINATES: protein motif:HMM:NF039847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOI80134.1"
                     /translation="TRAMQNFSKHISYQPKSENDIRRVLDDKKVDAVFNATPDHWHTP
                     GSVMAMKAGKHVYVEKPCSHNMFENEILVKAAQKFNKIVQMGNQQRSSDHTVEIMREI
                     HRGVIGNSYKAVAFYANTRGEAPLQKKAAIPAGLDWELFQGPAPRRDYTEETWDYNWH
                     WYGWDYGTAEAGNNATHELDVARWALNVDFPLNVEVEAAKQHFTNDGWEMYDTMDATF
                     RFADDKIIKWDGKSRNGHKTYGADRGTIIYGSEGTVFVNRDKYILYDRGGKVMKERTS
                     TSSEAGTALGGGGDMTTGHVINFFDTIRGKSKLTAPIDDASISNAMVHYANVAYRIGK
                     GFDIDDKTGRMFDREAMKLWNREYEPGWEPTLK"
BASE COUNT          847 a          900 c          776 g         1242 t
ORIGIN      
        1 tgtgagaggt ttaaggtgaa atccctttta tctactcgac tgagaggtcg gaaaataaaa
       61 taggaggaaa actattttat tttcctccta ctcgattacg tgaacgatgt gtaatccacg
      121 gtcaattaaa atcgtcagaa aacctgttta tttcctgatc actcatatct ccggcaataa
      181 taaccttgtg tttaaaagtt actttctcac cgctggacaa cttcaattca accgggtccg
      241 aattcttatc cacaaccttt ccccccagat tgttggctgc aaataatcca taacctcttg
      301 cgtgagacca tgccggataa ttagggttgt ttttattgtc catgataacg atggtgattg
      361 tttcgccttc cttatcagct tttaaagcaa cccatttgct cctttttccc cagacgtctc
      421 cccttgttaa accttctgca tttctgtaga atccatttac accttcgtta ttcagggccg
      481 gagtttctgt tacatttccg tttgcatcta aaaacttctc agctttttga gtcggttcct
      541 cgaaagttct gtctaccctc aaaccgatca gcccttcttt gttccctgta aaagttactt
      601 ccttatttgc tgtcagttct gtaactcttt caatcgtcct gatatcgttt ttcagccctt
      661 caaagatgta ggtcgtcttt tcattcaata aaatacttcc attattatct gtccattttg
      721 attgaacaac caatctccct ttccggttat ctgcttcaat aactttatca aacaaaatta
      781 tcccatattt atctttatct tccggtttta tggaatagga gttattccag aaatcaagtc
      841 cgtttacatc cccaaaatta aaccataatc caatgtgatg tggatgatcg gttctctcaa
      901 aaggccttgg attcaaggga aaccctcttg tgataaattt tccggagggt gtttttatag
      961 gataaagaca tggcttttcc aggttttccg gataaataaa cgatgtaaaa taaatatcgt
     1021 ttacgtatac ctcaactgcc tttttttctt cgctgacatt aaacctgacg tttgttgaag
     1081 tgccttggca gcttacatgc attatcagcg ctgcaatgat tagggcttta actgtttttt
     1141 tcatcttttg aattatagca acttattgac tttgtttcgt atgttcaata attgcaacaa
     1201 catatgtaaa agttcagttt aatacatacc tgttacaacc gcttgtattc tttttttcat
     1261 taatacttga atactttccc tcctgccatt acctgttttt gtttttcatc gaatatggcg
     1321 cgttgtccgg tgcgtaaggc ggccgtaacc ataatgtcgg cgatagagtg gttgtagcct
     1381 gcttccacgg gagcatttgt ttttacattg ccggccctca cacagtccat ccagtttttc
     1441 atatgtgcat ttgtgagcgg gtctgagcct gtatctgagc ttgtctccac acttattttc
     1501 ggtaattcga acgtgttaag cataaagggt ttcattccca tatcgcctgc gtgcctttct
     1561 gtcaatccgc catcgttgtt gatgatgtta gtgtccaggt caagttttcc tccgttagag
     1621 aaataccact cttttgtccc tcctgacgag ttattttgcc tggaggaata aattacctgg
     1681 aatccttctc ccggttcttt ttctttggcg tgtccataat caaaaacagc agtcatggta
     1741 tcgtagttgg ttcttccgtc gttccacatg tagatcccgc cgtttgccac taccgaccgg
     1801 ggataatcca gttctgagaa ccagtgtacg gtatcgatct gatgtgccat ccattggccg
     1861 ggtatgccgg atgaataggg ccagaacagg cggtattcca ggtattttcg cggatcccac
     1921 ggttcgtagg ggcggttcat caaaaagcgt ttccagtcgg tatcctgttc gcggatttct
     1981 ctggtgagtt ggggtaaacg ccatcgtccg ggctggttga cgttccatgt catttccacg
     2041 gcaatgatct tgccgaattt tcccgattta atgtagtcgt atgcagcgtg gtaatttgtt
     2101 ccgctccggc gttgtgatcc aacctgaata accttgttag tctctttggc tgctttcaac
     2161 gcaaagttag catcgtccat ggtttcagcg aaaggttttt ccacatatac atcacatccg
     2221 gccctgactg cttcggcggt atgaagcgca tgttgaaaat ctgcggtact gataattacg
     2281 gcgtcgggct tttgagacca gagctcatcg gtgtttctgg cggttctgag gtctcctccg
     2341 gcttgctcct tgtaccaggc ttgtccttcg tctcggcgac gattccaaat gtcgcagacg
     2401 gtaaagagtt cgaagttcat ctctttggca tatttgagga atgatttccc gagtgcacct
     2461 ctgaatcggt tggaaaaacc caatatgcct accctcactt tatcgttgga gccgatgata
     2521 ttggcataac ttttagccga catattcatc gtgcttccca gcgttattcc ggccacaccg
     2581 agtgccgatt tttttataaa atcacgtctt gaatttttca tctcatttaa agcttaaaat
     2641 tgatcgtatt gaaattaaat tatttcagtg ttggctccca tccgggttca tattcccggt
     2701 tccatagttt catagcttcc cggtcaaaca tacgcccggt tttgtcatcg atatcaaaac
     2761 ccttaccgat acgatacgct acattggcat aatgtaccat agcattactg atggacgcat
     2821 cgtcaatggg tgcggtaagt ttcgacttac ccctgatagt atcgaagaag ttgatcacat
     2881 ggccggtagt catgtcgcca cctccaccaa gtgccgtacc tgcttcactt gaagtagaag
     2941 ttctttcttt catcactttc ccccctcggt cgtaaagaat gtatttatcc cggtttacga
     3001 agacggtacc ttcactgcca taaataattg ttcctctgtc cgctccgtag gtcttgtgcc
     3061 cgttccggct tttgccgtcc catttgatta tcttgtcatc ggcaaaacgg aatgtggcat
     3121 ccatagtgtc gtacatctcc catccgtcgt tggtgaaatg ttgcttggcg gcttccactt
     3181 caacgtttag ggggaaatca acgttaagcg cccagcgtgc tacatccagt tcgtgtgtgg
     3241 cattgttgcc tgcttcggcc gttccgtaat cccagccata ccagtgccag ttataatccc
     3301 aggtctcttc agtataatcg cggcggggtg ccggcccctg aaacaactcc caatccagtc
     3361 cggcgggaat ggccgctttt ttctggagcg gtgcttcacc tcttgtattg gcataaaagg
     3421 ctacagcctt ataggaattt ccgataacgc cacggtgtat ctccctcatg atttcaacag
     3481 tgtgatccga ggaacgttgc tggttaccca tctgcactat cttgttgaat ttttgggcgg
     3541 cttttaccag aatttcattt tcgaacatgt tgtggctgca tggtttctca acatacacat
     3601 gtttgccggc tttcatggcc attacggaac cgggcgtgtg ccagtggtcg ggtgttgcgt
     3661 tgaatacagc atccactttt ttatcgtcga gcacccggcg tatatcgttt tcggatttcg
     3721 gttggtagct gatgtgttta gaaaaattct gcattgcccg ggtac
//
LOCUS       DAOSDR010000317         3755 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrimonas sp. isolate AMR_MDS_1826
            NODE_8634_length_3755_cov_4.275946, whole genome shotgun sequence.
ACCESSION   DAOSDR010000317 DAOSDR010000000
VERSION     DAOSDR010000317.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863355
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrimonas sp. (wastewater metagenome)
  ORGANISM  Petrimonas sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Dysgonomonadaceae; Petrimonas.
REFERENCE   1  (bases 1 to 3755)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3755)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:10:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,720
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 35
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3755
                     /organism="Petrimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8634_length_3755_cov_4.275946"
                     /isolate="AMR_MDS_1826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2023866"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..913)
                     /locus_tag="PLI30_11090"
     CDS             complement(<1..913)
                     /locus_tag="PLI30_11090"
                     /inference="COORDINATES: protein motif:HMM:NF024863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HOI80135.1"
                     /translation="MKYLFSFYIFIVGCATVASAQQTNIVFIGNSITQGVILDNPHEE
                     APPVQAVRWLEQRIPGKIEFRNCGISGSTTVNFLPASNTFFTRVKAAADELSKEGGTL
                     LFSIMLGTNDSALRGNLKSRMQPEQYYTNMKAITDELLRLYPSSRIVLNRPIWYSPTT
                     HNRSTYLQEGLEVLQSYFPELKKLAADYGSIFPGQVMLGDTAAFDHFKDNKPLFFKEE
                     GNSGTFYLHPNKTGAQKLGEFWGKAIKQAVQPKEWAEYSLGTVKDTPAMDKEDSRGML
                     APSVRVYLPDPDKATGRAVIALPGGGYQ"
     gene            1218..3116
                     /locus_tag="PLI30_11095"
     CDS             1218..3116
                     /locus_tag="PLI30_11095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019539737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HOI80136.1"
                     /translation="MSNQKEKLFTDFPPVSTEEWMEVINKDLKGADFQKRLVWKTNEG
                     FNVNPFYRAENIEGFSSAGNLPGQFPYVRSTRKDNVWYVRQDIDVNDYADANRKALSL
                     LDKGVTSLGFHLPKNDLSAENLKVLLNGIAPDKVELNFRTCISKTHELARLVVAYVTS
                     QNLDLLRCFGSIEYDPFRKILKKGVDVPNWMDDAAEMVKITASLPRYRSISITGNLLN
                     DAGAYSYQELGFSLSYANQLLGALIGKGLAPSLVAKKIKFELGVGSNYFMEIAKFRAG
                     RWLWAEIVNAYKPPCPPYIECENTAADGTCLCAAKMNIHASTSRFNQSLYDPYVNLLR
                     TQTEAMSATLGAVDSLTVRPFDEAFETPSEFAKRIAVNQQLLLKEEAHFDKVTDPSSG
                     SYYIETLTVSLAEQAWKLFLETEEKGFYEALKAGTVQDAIAASCDARFNAVANRKEIL
                     LGTNQYPNFTEKMAEKLADPKDHSCGCGTGDKTALKPLVIRRLAEPFNTLRLATEKSG
                     KTPKVFMLTIGNLAMRLARSQFSSNFFSCAGYQIIDNNGFPTVEDGVTAARNAGADVV
                     VLCSSDDEYVELAPKAFDLLKGGKEIFVVAGAPACMDDLKAHGIEYFIHVRSNVYETL
                     KGFNKRLL"
     gene            3132..>3755
                     /locus_tag="PLI30_11100"
     CDS             3132..>3755
                     /locus_tag="PLI30_11100"
                     /inference="COORDINATES: protein motif:HMM:NF013226.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prenyltransferase"
                     /protein_id="HOI80137.1"
                     /translation="MPKLKHWLMVAHPWSWPASGSPAMIAFSYVFYMYKTGVVAEVNW
                     LFGMLAIIGAVIFHAAGNLISEYHDYVRGVDKLEKTGPVRLIVLGIFEPKPVLLYGYL
                     VLSAGILLGIYLLVNTGFPFLIIGTIGIISSTLYYKFKYAGMSDLVIFICYGLSITLG
                     VVYVMTTELYWPILLISTPVGMLIVAILHANNTRDMLQDKEAGIKTQA"
BASE COUNT         1032 a          839 c          877 g         1007 t
ORIGIN      
        1 cctgataccc tccgccgggt aaggcaataa ctgctcttcc tgtagcttta tcgggatcgg
       61 gcaggtatac gcgcacagaa ggagcaagca tgcctcttga atcctccttg tccattgccg
      121 gagtgtcttt tacagttccc aacgagtatt ctgcccattc tttgggttga accgcctgtt
      181 tgattgcctt cccccagaat tcaccgagtt tttgcgcacc tgtcttgttc ggatgaaggt
      241 aaaacgttcc tgaattacct tcttctttga aaaagagcgg tttgttgtcc ttgaaatgat
      301 cgaatgcggc tgtgtctccc agcattacct gtccggggaa aatggagcca taatcggctg
      361 ccagtttctt caactccgga aaataagact gcaatacctc cagtccttcc tgcagataag
      421 tggatctgtt gtgtgtggtg gggctgtacc atatgggccg gttgagcacg atccggctgg
      481 aaggatataa cctcaggagc tcatccgtga tcgccttcat gtttgtataa tattgttccg
      541 gctgcatgcg agacttgagg tttccacgca acgcgctgtc gttagttcct agcataatgg
      601 agaagagaag cgtcccgcct tctttcgata actcgtcggc agcagctttc actcttgtga
      661 aaaaagtgtt ggaagcgggt agaaagttca ctgtagtcga gccacttata ccacaattgc
      721 gaaattcaat ttttcccggg attcgttgct caagccaccg tacagcctgc acgggaggcg
      781 cttcttcgtg cgggttatcc agtataactc cctgcgtgat gctgttaccg ataaatacga
      841 tgtttgtctg ttgtgcagag gcaacggttg cacagccgac gataaaaatg taaaaagaaa
      901 aaagatattt catacgatta aaattaaagc gaaaacccga gttaaaccat aacctcatcc
      961 ggaccgagga ttcagaataa ccgttttttc gtccaaattt acaaaacatt cgctaacaac
     1021 aataagcaaa agaaagaaaa ctattttttt gctgttaact tctcaaaaac aaatccctaa
     1081 aacacttttt taactaagca aaaaagagta attttgcaag cttattgaga gtaagaacaa
     1141 agacaatatt tttttctatc atcccttact ttccttgtta gcatgaaaag acctaaaata
     1201 ataaatatga gtaaattatg agtaatcaga aagaaaaact ttttaccgat tttcctccgg
     1261 tatccacgga agagtggatg gaagttataa acaaagacct gaaaggggct gactttcaaa
     1321 aaagactggt gtggaaaaca aacgaaggtt ttaatgtaaa tcctttctac agggccgaaa
     1381 acatcgaggg gttttcttcg gccggaaatc tgccgggaca attcccttac gtgcgcagca
     1441 caagaaagga caatgtttgg tatgtgcgtc aggatatcga cgtgaatgat tatgccgatg
     1501 ccaacaggaa agccttgtcg ctgttggaca aaggtgttac atcacttggg tttcatcttc
     1561 cgaagaatga cctttcggcg gaaaacctca aagttcttct caacggcatc gctcccgata
     1621 aagtggagtt gaacttcaga acctgtatct ccaaaacaca cgagcttgcc cggctggtgg
     1681 tggcatacgt tacttcgcaa aacctggacc ttctgagatg ttttggctcc atcgagtacg
     1741 atccgttccg gaagatcctg aaaaaaggag tggatgtacc caactggatg gacgatgctg
     1801 ccgaaatggt gaaaatcacg gcttcgcttc cccgctacag aagtatttcc attaccggaa
     1861 acttgctgaa cgatgccggg gcatacagct accaggagtt gggcttcagc ctttcctacg
     1921 caaatcagtt gttgggtgca cttatcggaa aagggcttgc cccgtcgtta gtagccaaaa
     1981 aaattaaatt tgaattgggt gtagggtcca actattttat ggaaattgcc aaattccgtg
     2041 cgggacgttg gttgtgggcc gagattgtga atgcatacaa gccgccttgc cctccttata
     2101 ttgaatgtga aaacactgct gcagacggca cttgcctttg tgcggccaaa atgaatattc
     2161 acgcttccac atcgcgattt aatcagtcgc tttatgaccc gtacgtgaat ttgctacgga
     2221 cacaaaccga agctatgtcg gctactcttg gtgcggtgga ttcgctgaca gtacgccctt
     2281 ttgacgaagc gtttgaaacg ccatccgaat ttgcgaaacg cattgccgtg aatcagcaac
     2341 tgttgttaaa agaagaagca cacttcgata aagttaccga tccttcttcg ggctcttact
     2401 atatagaaac attaactgta tcgcttgccg aacaagcctg gaaattattc ctcgaaactg
     2461 aagaaaaagg tttttacgaa gctctcaaag caggtactgt acaagatgcc atcgcagcat
     2521 cgtgcgatgc tcgttttaat gcagtggcca acagaaaaga aattctgctg ggaaccaacc
     2581 agtatccaaa tttcacggaa aaaatggctg aaaaattagc tgatcctaaa gatcattcgt
     2641 gtgggtgcgg tactggagac aagacggcac tgaagccact tgtgatacgg cgtttggccg
     2701 aaccgttcaa cacgcttcgt ttggctaccg aaaagagcgg taaaacaccg aaagtgttta
     2761 tgcttactat cgggaacctg gctatgcgcc tcgctcgttc gcaattctcc agcaacttct
     2821 tctcgtgtgc agggtaccag atcatcgata acaacggttt tccaacagtg gaagatggcg
     2881 taactgctgc ccgtaatgca ggagccgacg tggtggttct ctgttcaagc gacgacgagt
     2941 atgtggagtt ggctcccaag gcttttgatc tgttaaaagg cggaaaagag atctttgtag
     3001 ttgcaggcgc tcccgcttgt atggacgatt tgaaagctca cggaattgaa tacttcatcc
     3061 atgttcgcag caacgtgtat gaaacgttga aagggtttaa caagagatta ctctaacaat
     3121 ccaaacaaaa tatgcccaag ctcaaacact ggttaatggt tgcacatccc tggtcctggc
     3181 ccgcgtcggg atcaccggca atgatcgctt tttcgtacgt gttttacatg tacaaaaccg
     3241 gtgttgtggc tgaagtgaat tggctctttg gcatgctagc gataatcgga gcagtaatat
     3301 ttcatgcagc cggaaacctc atcagtgaat atcacgatta cgtaagagga gtggataaac
     3361 tggaaaaaac aggccccgta cgccttatcg ttctcggtat ctttgaaccg aagccggtgt
     3421 tgctgtacgg gtacttggta ctgtctgcag gtatcctcct gggtatttac ctgttggtaa
     3481 acacagggtt tccttttctt attataggaa caatagggat tatcagctcc acactttatt
     3541 ataagttcaa gtatgccggt atgagtgact tggtaatatt catctgttat ggattgtcta
     3601 tcactttagg cgtggtgtat gtcatgacaa cggaactcta ctggccgata ttgctgatca
     3661 gcacacccgt gggaatgctt atcgtagcta tactgcacgc taacaatacc cgcgatatgc
     3721 ttcaggacaa ggaagccggc attaaaacgc aggcg
//
LOCUS       DAOSDR010000318         3744 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrimonas sp. isolate AMR_MDS_1826
            NODE_8656_length_3744_cov_4.288967, whole genome shotgun sequence.
ACCESSION   DAOSDR010000318 DAOSDR010000000
VERSION     DAOSDR010000318.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863355
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrimonas sp. (wastewater metagenome)
  ORGANISM  Petrimonas sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Dysgonomonadaceae; Petrimonas.
REFERENCE   1  (bases 1 to 3744)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3744)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:10:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,720
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 35
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3744
                     /organism="Petrimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8656_length_3744_cov_4.288967"
                     /isolate="AMR_MDS_1826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2023866"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            383..2809
                     /locus_tag="PLI30_11105"
     CDS             383..2809
                     /locus_tag="PLI30_11105"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOI80138.1"
                     /translation="MGSDNSLEHRIGVKISLLFLIIILYVTGIFVYSFNLKKYVDTQK
                     EEISGAYNVLAQSNQLVLSVQEAQESLNAYLINPHRKIQLKYDSISTTISLQITELKK
                     LASQRNQDVYLESIDSLLHEKNKIVNQLIVQFRSRSPISELDKKIETYDQFIQDSVVV
                     TTNQDTTIVVKDKKGFWTRFKNLVNPNRAPDTIVSIARVEQETRLTSRIDTGVYADLK
                     NMSQAATKTYSSQLSGIEKQVRELILAEQNISLQISQLLTKFYNETTRVTREGIQSSE
                     LLTRKIFTFAVVVGALSLVLILIIIFLIVDDLKKGQKARLDLAREKQLTESLMESRHK
                     LLLSVSHDIKTPLSSMMGYMEMWQGEKIPESMKKQLLSAQNSGKHILSMLINLLEFSR
                     LERNAGELHNSRFNLTELVDEIIQMFRPLTEGKELDIEFENRVESPFYVETDHTVLRQ
                     ILINVISNAVKYTVKGRIDVRLSYEISLIFSISDTGIGMHESDIREIFKPFSRVKNPL
                     KAEGNGFGMYVTHGLIHSLKGEINVQSKKDEGTCVTITLPLHPAEKIPDESKNSHQDS
                     NNVYRKVLVFEDDITLGKLINEFLSKQGFKVKICNNTRDVDGFINHISLFDIVFTDMQ
                     MPDITGNEILLKIRRINSCIPVWLMSAYDDYNTEKAVSEGFSGFITKPIRMGKFIDVL
                     SGETQVITNHPPSLGEKFPQLASLFGDDTETIKKILSTFVTTVYKDTEHLTGLIEASD
                     FQGAQQLCHKIHPFISQIDAPHLCEVLIKMDSLRGEEETTYSSWKEDLSLSIRQIRAF
                     ADAIKKEYLF"
     gene            complement(2826..>3744)
                     /locus_tag="PLI30_11110"
     CDS             complement(2826..>3744)
                     /locus_tag="PLI30_11110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006843687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HOI80139.1"
                     /translation="VEGCSPLSTRMYSFINTVAPTSMAVMIIGESGTGKEHAARMIHE
                     RSQRAHGPFIAVDCGSLSIELAPSELFGHKKGSFTSAIEDKVGFFAEANGGTLFLDEV
                     GNLPYGVQMQLLRTLQEKKIRPVGATTDLEVDVRIVTATNENLEKAIAHGRFREDLFH
                     RLNEFMIEVPPLRSCKDDIPLYAEHFVDEANAELDKKVRFISAKVFSRLETYSWPGNL
                     RELRNVIRRAVLFSPGDTITLESLPFLSEKSVEENKEADVSLNVSPEEEKEKIVRALE
                     KTKGNKSRAAILLRIDRKTLYNKIRKYGL"
BASE COUNT         1158 a          845 c          764 g          977 t
ORIGIN      
        1 tttttactgg actacctatc cgtcttttcc aagtttaaac ataggaacta attttagaaa
       61 ttctcaatta agttttttct gtttaattaa acgtacaaaa aaagataatc aaataccgta
      121 caaaaaaact tcacaagtat gcagcggaag catatccatt taagaaacag cgaattatat
      181 tgaaaatagg acaaagcacc gtaccttaaa aactgtggac cgggtgtaga agtgtagaaa
      241 atctccacat ttttcggatg aaattccaca taaaatccag aggtgcatga caataagggg
      301 aagaaatgaa cacaagattc aggcaacggc acaacgaaac aaaaaatatt gtgtattttt
      361 gagcgaaaaa cagataatgc aaatgggatc ggataattct ttagagcatc ggataggagt
      421 gaaaataagt ttactgtttc tcattatcat tctttatgtc acagggattt ttgtctactc
      481 cttcaacctg aaaaagtatg tagacactca aaaagaagaa atttccggtg cttacaatgt
      541 tttagcgcaa agcaaccagc tggtactttc tgttcaggaa gcccaggagt cattgaacgc
      601 ctatttaata aatccgcacc gaaagattca gttaaaatac gattctatct cgacaacaat
      661 ttctctccag ataaccgaat taaaaaaact ggcatcacaa agaaatcaag atgtctacct
      721 ggaaagcatc gattcgctgt tacacgaaaa aaacaaaatc gttaatcagc ttatcgttca
      781 gttccgttcc cgaagtccta taagtgaact cgataaaaaa attgagacgt acgatcagtt
      841 cattcaggat tctgtggtgg ttacgacaaa ccaggacacc accatcgttg taaaggataa
      901 aaaaggattt tggacccgtt ttaaaaattt agtcaacccg aaccgtgctc ccgataccat
      961 cgtaagcatt gctcgggtgg aacaggagac ccggctcacg tccaggatcg ataccggagt
     1021 atacgccgat ttgaaaaaca tgtctcaagc tgccacaaaa acctattcat ctcaactgtc
     1081 gggcatcgaa aaacaggtac gggaacttat tctagccgaa caaaatattt ccttgcaaat
     1141 atcccagctg ttgactaaat tttacaacga gacaacacgc gtcacacggg aagggatcca
     1201 gagcagtgaa ctgctcacac gcaaaatctt cacgtttgcg gttgttgtag gtgccctgtc
     1261 gttggtattg atccttatta tcatttttct catcgttgac gacctgaaaa aagggcagaa
     1321 ggcccgcctc gatctggcca gggagaaaca gcttaccgaa tcgttgatgg aaagccgcca
     1381 caagttactt ctctccgttt cacacgacat taagactcca ctcagctcca tgatgggata
     1441 tatggaaatg tggcagggag aaaaaattcc ggaaagcatg aaaaaacaat tgctatcggc
     1501 ccaaaactcc ggaaaacaca tcctgagcat gctcatcaat ttactcgaat tctcccggtt
     1561 ggaacgaaat gcgggagaac tacacaacag ccggttcaat ttaacagagc tggtggacga
     1621 aattatacag atgttcaggc cgctgaccga agggaaagaa ctggatatcg agtttgaaaa
     1681 ccgggttgaa tcgcctttct atgttgaaac agaccacacg gttctcaggc agatattgat
     1741 aaacgttatc tccaatgcgg taaaatacac cgttaaggga agaatcgatg tccggttaag
     1801 ttacgaaatc agcctgattt tttccatcag tgacaccgga atcggcatgc atgaaagcga
     1861 tatccgggag atattcaaac cattttcccg tgtgaaaaat ccgctaaaag ctgagggaaa
     1921 cggctttgga atgtacgtaa cgcatgggct tatccattcg ctgaaaggtg aaataaacgt
     1981 ccagtcgaaa aaggacgaag gcacctgcgt tactataaca ctgcccctgc accccgcaga
     2041 aaaaataccg gatgaaagta aaaacagcca tcaggacagc aacaacgttt acaggaaagt
     2101 actcgttttt gaagacgata taaccttggg gaaactgata aatgaatttc tctcgaaaca
     2161 gggatttaag gtaaaaatct gcaacaacac ccgtgacgtg gacggcttta taaatcacat
     2221 atccctgttt gacattgtat tcacagacat gcagatgcct gacatcaccg gaaatgagat
     2281 tttgctcaaa atccgtagaa ttaattcctg cattcccgtc tggctgatga gtgcctacga
     2341 tgattacaac accgaaaagg ccgtttcgga aggattcagc ggatttataa caaagcccat
     2401 ccggatgggc aagtttattg atgttctctc cggagaaaca caggtcatta cgaaccatcc
     2461 tccctcgctt ggtgaaaaat ttccgcaact ggcttcttta ttcggagatg acaccgaaac
     2521 catcaaaaaa atcctatcaa ccttcgttac aaccgtctat aaggataccg aacatctgac
     2581 cgggttgatt gaagcatccg actttcaggg agctcaacaa ttgtgccaca agatccatcc
     2641 ttttatcagt cagattgatg cccctcattt gtgtgaggtt ttgataaaaa tggactcact
     2701 caggggagag gaagaaacta cctattcttc ctggaaagaa gatttgtcgt taagcattcg
     2761 tcaaataaga gcgtttgccg atgctattaa aaaggaatat ttattctgac aatcgtcatt
     2821 ttccgctata agccgtactt gcgaatcttg ttgtacagag tttttcggtc tatacgcaat
     2881 agaattgctg cccgggattt gtttcctttg gttttttcca gagcacgtac tatcttttct
     2941 ttttcttctt cgggcgagac gttcaaggat acatctgcct ctttattctc ttcaacactc
     3001 ttttccgata aaaacggtag actctccaac gtaatcgtat caccgggtga aaaaagtacc
     3061 gcacggcgaa ttacatttcg cagttccctt aggttgcctg gccaagaata ggtttccaac
     3121 cgggaaaaga ccttagccga gatgaacctc acttttttat ccagttcggc atttgcttcg
     3181 tcaacaaaat gttcggcata caacggaata tcgtctttgc aactcctcag tggcggaacc
     3241 tcaatcatga actcattgag tcgatgaaac agatcctctc taaaacggcc atgggcaatc
     3301 gctttctcga gattttcatt ggtagccgta acaattcgca catctacctc caaatcggta
     3361 gtcgctccca ccggccggat ctttttttcc tgcaaggtcc tgaggagctg catctggacg
     3421 ccgtacggca agtttcccac ttcatccaga aataacgtac cgccatttgc ttctgcaaaa
     3481 aagcctactt tatcttcaat ggcggaggta aaagaacctt ttttgtgtcc gaacagctca
     3541 cttggcgcca gttcaatgga taagcttcca caatctactg cgataaacgg gccatgggca
     3601 cgctggctcc gttcgtgaat cattcttgcc gcatgttcct ttcccgtacc gctttcgcca
     3661 ataatcatca cggccattga agtgggagca acggtgttta taaaagagta catacgggtg
     3721 gacagcggac tgcatccttc tacc
//
LOCUS       DAOSDR010000319         3743 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrimonas sp. isolate AMR_MDS_1826
            NODE_8667_length_3743_cov_4.341920, whole genome shotgun sequence.
ACCESSION   DAOSDR010000319 DAOSDR010000000
VERSION     DAOSDR010000319.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863355
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrimonas sp. (wastewater metagenome)
  ORGANISM  Petrimonas sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Dysgonomonadaceae; Petrimonas.
REFERENCE   1  (bases 1 to 3743)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3743)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:10:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,720
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 35
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3743
                     /organism="Petrimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8667_length_3743_cov_4.341920"
                     /isolate="AMR_MDS_1826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2023866"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(1..628)
                     /locus_tag="PLI30_11115"
                     /pseudo
     CDS             complement(1..628)
                     /locus_tag="PLI30_11115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005857688.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TGS domain-containing protein"
     gene            complement(710..1771)
                     /locus_tag="PLI30_11120"
     CDS             complement(710..1771)
                     /locus_tag="PLI30_11120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019539422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HOI80140.1"
                     /translation="MTTIIRTGIIGYGLSGRVFHAPFVDVVDGYELTKISTRKPDSIS
                     MINKRYPSTVIVPDGQDIIDDPDIDLAIVTSPNTDHFRWAKAALLVGKHVLVEKPFTV
                     TVAEADELIELAKKQGKVLTVYHNRRFTSDTKTVRKLLDSGLLGEVRDYASHFDRYRP
                     EPRPFGAWREKPLPGSGIFYDLGAHLIDQALWFFGMPEAVTAEINSQRPWAKADDHFD
                     VRLHYPAFTATLKSGMLCKIPGPTYMIHGTNGSFVKYGLDVQEATLDGGAIPEGKNWG
                     REPEEIWGTVNAEYKGIKIQGKLESEHGDYREYFMNLRDAIWGKAAIAVKPEEARNVM
                     RIIELSFRSSEERRTISVK"
     gene            complement(1812..2387)
                     /locus_tag="PLI30_11125"
     CDS             complement(1812..2387)
                     /locus_tag="PLI30_11125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005639618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009143 - nucleoside triphosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-canonical purine NTP diphosphatase"
                     /protein_id="HOI80141.1"
                     /translation="MKIVFATNNKHKLHEIRKITAGSMEILSLSEINCHEEIPETGAT
                     LEENALMKAQFIKDKFGFDCFADDTGLEVDALDGAPGVYSSRYAGEACDPQDNMDKIL
                     RVLQGERNRNARFRTVIALLLNGERHFFEGEIKGQIIEEKRGTAGFGYDPVFLPDGYK
                     QTFAELGDDEKNKISHRAIATRKLVEFLLSN"
     gene            complement(2384..>3743)
                     /locus_tag="PLI30_11130"
     CDS             complement(2384..>3743)
                     /locus_tag="PLI30_11130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI80142.1"
                     /translation="VYNRHLTDFPESIRRKEVYHQLYQLYLRMGNRAMAKSLKDKIIS
                     EFPQSEYAAAMSDPDYEHVVRNYARVQDSLYQQTYLAYQQGQVETVQGNFKRAQKLFF
                     NGHLMPKFMLLNALSLAQSGNPEQLNTALSELIQKHPGTEETKMAQQILAGLSEGKTL
                     VANVQPFSGIDWESHIVAGNAADSVCFEKEKNLPHSLLLLFSPNSLRKNELLFAVSDF
                     NFSNFQIRSFHTDYTYVFPYEALQIKPFRSFEEAGRYASMLAGDSIFRQNITTGIVPL
                     VISDKNLEILRDGEPLENYISFVTNELSDGLSDSIPLIVSGKIPQTITETEKNVPLEP
                     EKTGKPDIASKKPTPKQSERLTDEQRLEELVRKAEEALRQTGDVFSKRDRERILKERE
                     EARKEWIKQRERDLKQREKVRKEILKQRERERKQKLKEQERLRKEKLKERERIVQQRN
                     EK"
BASE COUNT          864 a          942 c          786 g         1151 t
ORIGIN      
        1 ttcatcgcca cgccaatatg ctcctgctac gctcaacact tttactgact ttatgtacga
       61 ggtattgggt aaatgcgggc cacgacataa atcggtaaaa tccccctgag tatatgtggt
      121 tggttccgtc ttccagttca ctgataagtt ccactttgta gttctcattg cggtcggaga
      181 acattttaat ggcatcagct ttcgatatat tttttcgcct gatctcttcc ttggccgcga
      241 taagttccat cattttcttt tcgatagcgg gaaaatcaga ttctttaatg gatacgccct
      301 ctcccggatc aacatcgtaa taaaatccgt tttcgatggc cggcccgatg ccgaatttaa
      361 tgcctggata aagtgcctgc agcgcttctg ccatcaagtg cgccgacgag tgccaatagg
      421 catgtttacc ttcggtatcc tcccacttga gcaattgaat ggcagcatct tcactgatgg
      481 gtcgggttag atcccacgtt tcaccgttta cgcttgccgc caggacttct tgggccagtc
      541 gtgaactaat actctctgca atttgtaatc ctgtaattcc ttcttcgtat tcccgaaccg
      601 aattatcggg aaatgtaatt tttatcatag cgtgtatatc gttttcttcc aatttttttt
      661 cgaactgcaa atgtaatgat tttgtggatt atagcgtact tatttttttt tattttacgc
      721 ttatcgttcg cctttcctca ctgcttcgaa atgacaactc aataatcctc attacgttgc
      781 gggcctcctc gggttttacg gcaatcgctg cttttcccca aatagcatcg cgaagattca
      841 tgaaatactc ccggtaatca ccgtgctcac tttctagttt tccctgaatt ttaatgcctt
      901 tgtattccgc attcaccgta ccccagattt cctcaggttc gcgcccccag ttttttcctt
      961 cgggaatagc tcccccgtcg agggttgcct cctgcacatc caatccgtat ttcacaaacg
     1021 aaccgttcgt cccgtggatc atgtatgtcg gccccggaat tttacaaagc atccccgatt
     1081 tcaacgtagc ggtaaaagca ggataatgca acctcacgtc aaaatgatca tcggcttttg
     1141 cccaggggcg ttgcgaattt atttcagcag taactgcctc gggcatcccg aaaaaccaaa
     1201 gagcctgatc gataaggtgt gcccccaagt cgtaaaaaat tcccgacccg ggaagtggtt
     1261 tttccctcca agctccgaaa ggacggggct ccgggcgata acggtcgaag tgtgacgcgt
     1321 aatcgcgcac ttcacccagt aatccgctgt cgagcaattt ccttaccgtt ttggtgtcgc
     1381 tggtaaaacg gcgattgtga tagacggtta gcaccttgcc ctgttttttc gccaactcga
     1441 ttaattcgtc ggcttcggcg accgtcacgg taaacggctt ttccaccagg acatgcttac
     1501 ccacaagcaa agccgccttt gcccaacgga aatggtcggt attgggcgat gtcacgatag
     1561 ccaaatcgat atcgggatca tcgataatat cctgcccatc aggaacaata accgttgatg
     1621 gatacctctt gttgatcata ctgatactgt cgggcttacg tgtactgatt ttggtgagtt
     1681 cgtaaccgtc cactacatct acaaacggtg cgtggaaaac tcttccggat agtccgtatc
     1741 cgataatgcc tgtgcgaatg atagttgtca tgtttgtcag tttaagtcgt tggttattca
     1801 aaggtaatta attaattcga caaaagaaac tccaccagtt tgcgggtagc tatagccctg
     1861 tggctgattt tgtttttttc gtcatctccc agctctgcaa atgtttgttt gtaaccgtcc
     1921 gggagaaaga ccggatcata cccaaacccg gctgtcccgc gtttttcttc tataatctga
     1981 ccttttattt ctccttcgaa gaaatgcctc tccccattca acaacagggc aataacagtt
     2041 ctgaaccggg catttctgtt tctttctccc tgcaacacac gcaagatttt atccatatta
     2101 tcctgcgggt cacaagcttc gccggcatat cgcgacgaat aaacaccggg cgctccgtcc
     2161 aatgcatcca cttccagtcc ggtatcatcg gcaaagcaat cgaagccaaa cttatccttt
     2221 ataaactgcg ccttcatcaa tgcattctct tcgagggtcg ctcctgtctc agggatttct
     2281 tcgtgacagt tgatttccga cagacttaaa atctccatgc tgccagcggt tatctttcgg
     2341 atctcatgga gtttgtgctt gttgttggtg gcgaatacaa ttttcatttt tcgtttcttt
     2401 gttgcacgat acgttcgcgc tcttttaatt tttctttgcg caaccgctcc tgctctttca
     2461 atttttgctt gcgctcacgc tcgcgttgct tcagaatttc ttttcggact ttttcccttt
     2521 gtttcaaatc ccgttcacgt tgttttatcc actctttacg ggcttcctca cgctctttca
     2581 atatcctctc cctgtctctt ttcgagaaaa cgtcaccggt ttgccgaagc gcttcttccg
     2641 cctttcttac caattcttcc aggcgttgct catcagtcag ccgttcggat tgtttaggtg
     2701 ttggcttctt tgaagcaata tccggtttac cggttttttc aggttccaaa ggtacgtttt
     2761 tttctgtttc cgtaatcgtc tgcggtattt tccccgaaac aataagcgga atagagtcag
     2821 acaggccatc acttaactca ttagtaacga aagatatata attttccaac ggttctccgt
     2881 cccgaagaat ttccaggttt ttatcggaaa taactaacgg aacaataccg gtggtgatat
     2941 tttgccgaaa aatggaatct cctgcaagca tgctggcgta tcggccggcc tcttcaaacg
     3001 aacggaacgg tttaatctga agcgcctcgt acggaaagac ataggtataa tccgtgtgga
     3061 aagaccggat ctggaaattg gagaaattga aatcggaaac ggcaaagagc aactcatttt
     3121 tacggagcga gtttggtgag aacagaagca ataaactgtg ggggaggttt ttctcctttt
     3181 caaaacaaac ggaatctgcg gcattacctg ccacaatgtg gctttcccaa tcgattcccg
     3241 aaaagggttg tacatttgca actagcgttt tcccttccga aagacccgcc aggatttgtt
     3301 gagccatttt cgtttcttcg gtacctggat gtttttgaat gagctcgctt aaagcagtat
     3361 tcaactgttc cggattaccc gattgtgcca acgataaagc gttcagcaac ataaatttcg
     3421 gcatcaggtg tccgttgaag aacagctttt gtgcccgctt gaaatttccc tgaaccgttt
     3481 ccacctggcc ttgctggtat gccagatacg tctgttggta aagcgaatcc tgaacccggg
     3541 catagttacg cacgacatgt tcgtaatccg ggtcactcat ggccgcagca tattcacttt
     3601 ggggaaattc agaaattatt ttgtccttta agcttttagc cattgcccgg tttcccattc
     3661 tcaggtaaag ctggtaaagc tggtgataaa cctcttttcg ccggatgctt tccggaaaat
     3721 ccgttaagtg gcggttgtaa acc
//
LOCUS       DAOSDR010000320         3737 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrimonas sp. isolate AMR_MDS_1826
            NODE_8684_length_3737_cov_3.852797, whole genome shotgun sequence.
ACCESSION   DAOSDR010000320 DAOSDR010000000
VERSION     DAOSDR010000320.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863355
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrimonas sp. (wastewater metagenome)
  ORGANISM  Petrimonas sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Dysgonomonadaceae; Petrimonas.
REFERENCE   1  (bases 1 to 3737)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3737)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:10:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,720
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 35
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3737
                     /organism="Petrimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8684_length_3737_cov_3.852797"
                     /isolate="AMR_MDS_1826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2023866"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..528
                     /locus_tag="PLI30_11135"
     CDS             <1..528
                     /locus_tag="PLI30_11135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI80143.1"
                     /translation="SIANLYNGFKPKENPIDSVDDVKQIVRFLKNSHDYYRNDKYPQI
                     SAYIMQLQKNHPEKELKLLEQFFNEYFAEVIDHLDYEDNVAFPYFIEFLGNRDNLESN
                     AYSAQEYSEHHTDIELKIKDIKNLLLKYVVINDDLNLRRKLLFSLCELEYDLYIHSLI
                     EETILIPFGYNMEKA"
     gene            525..1133
                     /locus_tag="PLI30_11140"
     CDS             525..1133
                     /locus_tag="PLI30_11140"
                     /inference="COORDINATES: protein motif:HMM:NF012422.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HOI80144.1"
                     /translation="MNRHRLHIAIMVHSQIIYEGLHAVLSQSDIDCVICKVDSLDDLE
                     NILPSKKVDLLIINPLLLVNREKDVRRIRKNHPNFSIVGIHLGIVDNHSLALLDSSFT
                     IFDTSEQVLSRLQKTGTNSELKFPSNDDNLTERELDVLTQLVHGYSNKEIADSLNISI
                     HTVVTHRKNITAKTGIRSQSGLTIYAISKKIIQIEDVDLQSH"
     gene            complement(1138..1665)
                     /locus_tag="PLI30_11145"
     CDS             complement(1138..1665)
                     /locus_tag="PLI30_11145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI80145.1"
                     /translation="MEPQKKKQNIYHIMRVLHRDIGFLTIGLTLVYALSGILLIYRNT
                     DLLKTEKVEEKQLATNLSGNDLGQQLRIRNFKVEREEGETIYFKEGFYHAATGKSVVT
                     RKVYISPFDKLVDLHKITGTHRVSVLSLVYGFMLCFLAVSSLFMFKFGSRKSRRGIAM
                     IALSIVITVAIVALV"
     gene            complement(1684..>3737)
                     /locus_tag="PLI30_11150"
     CDS             complement(1684..>3737)
                     /locus_tag="PLI30_11150"
                     /inference="COORDINATES: protein motif:HMM:TIGR01783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0015343 - siderophore-iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TonB-dependent siderophore receptor"
                     /protein_id="HOI80146.1"
                     /translation="RERPEKMEALTRIPLRLDEQVQSVSVISQKMIAEQGAMTLNDAV
                     RNAPGLGTFATYGNTSESITSRGYRGIPVVKNGVRIHSDFRGQGFLSDMQGVETVQVL
                     RGSAAIAQGIGNDIGSAGGVVNVATKTPRFVNSGNLGLRIGSWGQVRPTFDIQRVLDV
                     HGHAAFRINGAYERADNYRVHVSKDRFYVNPSFAWRPDEKTNIVLEMDYLHDSRTPDQ
                     GTVNLSADSVNNIYKMPADKFLGFSSDRQFTNTLTYMAHLTRELGNGFSVRVAYAGAD
                     LNSRSVRGHVSALRSAGTTGKYNLRSRNYSGGQRSDKNGVFQADLIGKDIQTGSVKHT
                     FNVGFDYRWSDVSTVSTNSVTVDTIDVLQAIPNVMPNVVLKDSDPSTSQSYSYGLLLQ
                     EVMTFNRYLKVSLGLRYSQVNGLSDNVVSSTGGDVWDPLFGIIVTPYENFNLFASYTT
                     TTSLRGAANLLEDRVTPVGPTREKQVEVGLKTEWLDNRLRFNATYFHIQNNNLTYSLL
                     NDAGQNTGYYMKAGNLKREGVELELTGRLLKNLDVVAGYSYLDAAYHDSPYYHAGSAP
                     MNTAKNTANGWVNYSIFDGVLCNLSFGLGMYYVGQRPLAEYTYRVVPGHAVQANTRPF
                     LADAYTTVNAQVGYRFRNYRIRVFMNNLFDSTGYTAYYRGGFLNPTDPRNVAVALNYQ
                     F"
BASE COUNT         1028 a          951 c          825 g          933 t
ORIGIN      
        1 tcgattgcca acctgtacaa cggcttcaaa cccaaagaaa atcccattga ttccgttgac
       61 gatgtaaagc agattgtacg ctttttgaaa aacagtcacg actattaccg caacgataaa
      121 tatccgcaaa taagtgccta cataatgcag ctgcagaaaa accatcctga aaaggaatta
      181 aagttgctgg agcagttttt taacgaatac tttgctgagg ttatcgacca cctggactac
      241 gaggacaatg tggcttttcc gtattttatc gagttcctgg gcaacaggga taatctggag
      301 agcaatgcat actcggccca ggaatacagc gaacaccaca ccgatataga gctgaaaata
      361 aaagacataa aaaacttatt gttgaaatac gtagtaatca acgacgacct gaacttacgc
      421 cgtaaactgc ttttctcttt gtgtgaattg gagtacgacc tctatatcca ctcattgatt
      481 gaggaaacca ttttaattcc tttcggatac aatatggaga aagcatgaac agacaccggt
      541 tacatatcgc catcatggtg cattcgcaga ttatttacga aggattacac gcggtacttt
      601 ctcaatcgga tattgactgc gtaatctgta aagtggattc actggacgat ttggaaaata
      661 ttctcccttc aaaaaaggta gatttattga ttatcaaccc tctactattg gtgaacaggg
      721 agaaggacgt aagaaggatc cggaaaaatc accccaattt ctccatcgtt ggaattcact
      781 taggcattgt cgataaccat tcactggcgc tgctcgattc gtcttttacc attttcgaca
      841 catcggagca ggttttgtcc aggttacaaa aaacgggaac caacagtgaa ttgaaattcc
      901 cgtcaaacga tgataacctg actgaaagag aacttgatgt acttacccaa ttggttcacg
      961 gttactccaa caaggaaatt gccgactcgc tcaacatcag tatccatacg gtagttaccc
     1021 accggaaaaa cataacagcc aaaaccggca tacggagcca gtcgggatta accatatacg
     1081 ctatctccaa aaaaattatt caaatcgagg atgttgacct gcaaagccat tgagtcgtca
     1141 caccaacgcc acaatcgcca ccgtaataac aatgcttaat gcaatcatcg ctattcccct
     1201 ccggcttttc cggcttccga acttaaacat aaacaacgag gaaaccgcaa ggaaacaaag
     1261 cataaatccg taaacaagag acagcacgga cactctatgc gttccggtaa tcttgtgcaa
     1321 atccaccaac ttgtcaaacg gagagatata cactttgcgc gttacgactg attttccggt
     1381 agcagcatgg taaaaacctt ccttgaaata aattgtttcc ccttcttccc tttccacttt
     1441 aaaattccga atacggagct gttgtcccaa atcattcccc gaaagattcg ttgccagttg
     1501 cttttcctct actttctccg tcttcagcaa gtcggtattc cgataaataa gcaaaattcc
     1561 cgaaagcgca taaacaaggg tcaacccgat ggttagaaat ccgatatcgc gatgtaatac
     1621 ccgcatgatg tgataaatgt tctgcttttt cttttgaggt tccatttttt tctaccttag
     1681 ctgttaaaac tgataattca gcgccaccgc aacattccta ggatctgtcg gattgaggaa
     1741 accaccgcga taatatgctg tgtagcccgt ggaatcgaac agattgttca taaacacccg
     1801 gatgcggtaa tttctaaacc ggtaacctac ctgcgcattc acggtagtat atgcatctgc
     1861 aagaaatgga cgcgtgtttg cctgcacagc gtgtccaggc acaacgcgat aggtatattc
     1921 ggccagcggg cgttggccca cgtaatacat gcccaagcca aaggaaagat tgcataagac
     1981 gccatcaaaa atactataat ttacccatcc gttggcagta ttcttggcgg tgttcatcgg
     2041 agcagaccct gcgtgataat aggggctatc gtgataagcg gcgtccaggt aggaatagcc
     2101 cgccacgaca tccaggtttt tcagcaaacg ccctgtaagt tccagctcca caccttccct
     2161 cttcagattt ccggctttca tgtagtaacc cgtgttctgt ccggcatcgt taagcaacga
     2221 ataggtcagg ttgttgttct ggatgtggaa ataggtcgca ttgaaacgaa ggcggttgtc
     2281 aagccactcc gttttcagcc ccacttcaac ctgcttttca cgcgtggggc cgacgggcgt
     2341 aacacggtcc tcgaggaggt tcgcggcgcc acgcaacgat gtagttgtcg tatacgatgc
     2401 aaaaaggttg aaattttcgt acggtgtaac gatgatgcca aacagcggat cccacacatc
     2461 accgccagta gacgagacaa cgttgtcgct caatccgttc acctggctat atcgtaatcc
     2521 tagcgacact ttcagataac ggttaaacgt cattacttcc tgcaacagta aaccgtagga
     2581 gtaggattgc gacgtggacg gatcgctatc cttaagcacg acattgggca tgacattggg
     2641 aatagcttgc agcacgtcaa tcgtgtctac ggtaacggaa ttggtgctga cagtggaaac
     2701 atccgaccag cgataatcga accccacatt aaaggtatgc ttcacagagc cggtttgaat
     2761 atctttacca atgaggtctg cctgaaagac tccgttcttg tcgcttcgct gtccgccgct
     2821 gtaatttctc gatcgcaggt tgtacttacc tgttgtgccc gcagatcgca atgccgaaac
     2881 atggccccgg acggaacgcg agttcaggtc ggcaccggca tacgccacgc gcacgctgaa
     2941 accgtttcca agttcgcgcg tgaggtgcgc catgtatgtc agggtgttgg taaactgacg
     3001 gtcgcttgaa aaacccagga acttatcggc cggcattttg tagatattgt tcacactgtc
     3061 ggcactcagg ttcacggttc cctggtccgg ggtacgcgaa tcgtgcaggt aatccatttc
     3121 aagcacaatg ttggtttttt catcgggtct ccacgcaaat gacggattca cgtaaaagcg
     3181 gtctttcgag acatgtacac ggtaattatc cgcccgttca tacgctccgt tgatgcggaa
     3241 agcggcatgt ccgtgcacat ccaacacacg ctggatatca aacgtggggc gaacctgtcc
     3301 ccaactgccg atacgcaagc ccaggttccc ggaattgaca aaacggggtg ttttggtagc
     3361 aacgttcact accccacctg ccgaaccaat atcgttcccg atgccttgcg caatggcggc
     3421 agaaccgcgc aacacctgca cggtttcaac cccctgcatg tccgaaagaa aaccctgccc
     3481 acgaaaatcg gaatggatgc gcaccccgtt tttcaccacg gggatccccc gataaccacg
     3541 cgaagtaatg ctttccgatg tattgccgta agtggcaaaa gtccccagtc cgggtgcatt
     3601 tcgcacagca tcgttgagcg tcatcgcgcc ttgttcggca atcatttttt gcgatatcac
     3661 agatacactt tgcacctgct cgtccaacct aagcggtata cgcgtgagcg cttccatttt
     3721 ttcgggacgt tcgcgcc
//
LOCUS       DAOSDR010000321         3732 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrimonas sp. isolate AMR_MDS_1826
            NODE_8695_length_3732_cov_2.903182, whole genome shotgun sequence.
ACCESSION   DAOSDR010000321 DAOSDR010000000
VERSION     DAOSDR010000321.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863355
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrimonas sp. (wastewater metagenome)
  ORGANISM  Petrimonas sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Dysgonomonadaceae; Petrimonas.
REFERENCE   1  (bases 1 to 3732)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3732)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:10:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,720
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 35
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3732
                     /organism="Petrimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8695_length_3732_cov_2.903182"
                     /isolate="AMR_MDS_1826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2023866"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(130..744)
                     /locus_tag="PLI30_11155"
     CDS             complement(130..744)
                     /locus_tag="PLI30_11155"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018109334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006486 - protein glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HOI80147.1"
                     /translation="MYRNFIKPFFDFLISITLLLVLSPIFLVLIIVLTLNNSGKPFFF
                     QTRPGKNARLFKVIKFKTMNDKKDVSGTLLPPAQRITSVGRFIRSTSLDEIPQLLNVI
                     KGDMSLVGPRPLRVEYLPLYSAFQARRHEVKPGITGWAQVNGRNSLTWEEKFKLDVWY
                     VDNQSLWLDLKILFLTFWKVIARKDINTSNEIIMEPFKGAKLDG"
     gene            complement(756..1969)
                     /locus_tag="PLI30_11160"
                     /pseudo
     CDS             complement(756..1969)
                     /locus_tag="PLI30_11160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009018255.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
     gene            complement(2664..3674)
                     /locus_tag="PLI30_11165"
     CDS             complement(2664..3674)
                     /locus_tag="PLI30_11165"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOI80148.1"
                     /translation="MLLTIEASNIYFRGGFVLLEQLLEYCEQHSLEANVYIGYQEVFD
                     YFGKKKYKNISLIKTNGLKTFLRYFWKRSNTLFFCNLPPFVKCEKSVLYAHNILFFES
                     PRPVKGAALVFNLKKFVYFYWIKYFARNVDVVACQTEAVQKSLWENMQVKSELYPFYR
                     EASPLPLERVYEFCYVGSGAPHKNNNRLLEAVDKLSEKYSFRLVMTINDSDTGNVLNE
                     KIDAINRKYKRTVVVNKGYMPYDEVREIYSSTRALIFPSQAETIALPLIEGLQFGLKI
                     LSSDLPFTHQVVENPIVFNPESVDEIVNVMENQLKGRYKDIIQKNRIPNKLPELINCL
                     RY"
BASE COUNT         1044 a          859 c          672 g         1157 t
ORIGIN      
        1 agcccattat aaatccttcc aattttttca gtatcatcat caaggaaacc tatcagatta
       61 taggctcctg tatctttgat atacgaagca taaacctgac cataagttcc tgcgccaaca
      121 ataacagctt tatccatcaa gttttgcacc tttaaatggt tccattataa tttcattgct
      181 cgtatttatg tctttccggg caataacttt ccaaaaagta agaaataata tttttaaatc
      241 cagccaaaga ctttgattat caacgtacca aacatctaat ttaaattttt cctcccaggt
      301 caatgaattg cgcccattca cttgcgccca gccggtgata cccggtttaa cttcgtggcg
      361 acgcgcctga aaagccgagt acagtggtaa atattccaca cgtaaaggcc tgggaccaac
      421 caaactcata tcccctttga ttacgttcag cagttgaggt atttcatcaa gcgatgtgga
      481 ccggataaac ctgcctacag aagtaattcg ttgtgctggt ggcaagagtg tacctgagac
      541 atccttttta tcgttcattg ttttgaactt gatgacctta aaaagacggg cattttttcc
      601 aggacgtgtt tgaaagaaaa atggtttacc agaattattc agcgtcagaa caattattag
      661 aactaaaaaa ataggggaca atactaacaa taaggtaata cttatcaaaa aatcgaagaa
      721 gggttttatg aaattgcgat acattagttg cttccttatt gatcactctc aaaatgagac
      781 atgatgatat tataactttg cgcggctgta tagttgttaa tcagatagtt atatccattg
      841 tttcccattc ggttgattaa ggccggatct tgtattagcc tggtaatgtt cgatttaaaa
      901 ccctctgcgt cgccattaag cacccaaaag ccgtaattgt tttcagccga tattttacct
      961 atgtctgtat ttgagtcggt tgctgaaatc acaggcatgt ggttttccag atacgaaagc
     1021 aagcgggagg ggtaattcgg aatggtgaat cttttgtcaa gaaatattaa tccgacatcg
     1081 catgcacgta ccagccgatc gtattcgggt tttgtaatgc ctttattaat tgggcattcg
     1141 aaggcatttt ctgatcaaac cagcgtttga tttttgcata ttccgttccg gatcctgcaa
     1201 caataaaata tgcctcctca attggtgtgc atgcctccat catctccaat aagaaatcaa
     1261 gaccctgtgg tttgcccaga tttccaccat aaataaatat tgttttattc tccggaagac
     1321 ccaattctct gcgaagttgc atcttgtccg gctctgcttt agaatcctct gccagctcaa
     1381 tgctgttcgg gtttacttca acatttttgg agtaaatttt cggattgtgc tgtagcaaat
     1441 attgaacatt ggcaggagac atgcagccga tatagtccga aaccttgtac agctgtttct
     1501 ctttcctctg gaaaaagcgg tgcaggatac aatccctttt catcatgccc agatcaaccg
     1561 cattctgtgg aaaaatatct ttcaacagca aataactttt agcgttatct ctttttttaa
     1621 taaactgaat aaccctgctg aaggttatag gtggagtaga ataaagcacc aggtcaaatt
     1681 tcttcgtgct caggtatttt ttaatagccg ataagaactg gtactccagc agtaccgtac
     1741 ccacaccttt ttctatcaga tttgttttgg ttacattcag ggtttttacc tgcaggatag
     1801 aaactccgtc tttctgcctg taattggcag ctatcccttt tcggcgttcg acgggtgtca
     1861 ctatcgtaac atcgtgtcct ttattccgaa actgacgcag caaatcctgg taaattcccc
     1921 tgtcttcgat ggagtttatt ccaaccagcg ttacaaaaag tacattcatc tgttttggtt
     1981 tatactacgt gtgttctacg tatttttttt tgcatatgtt ttttagttcc ctcactcctc
     2041 tgcgttccgt tcgggatgac agccgttccg cttagtcaaa taccgtcgcc ctatccattc
     2101 tacattcgtc cagttcctac cggcgacggt gcgaaattct ccgagtaccc gtcgtgctaa
     2161 aaactacatt atacactaca atccgtatcg acgggtaata gataatgaat gaccctgtca
     2221 ttccgaacgc agtgaggaat ctgatttgat ccctccttac agtctgcctg ccttttagtt
     2281 cccctcactc cactgcgttt cgcttcggga tgacagtcgt tccgcttagt caaataccgt
     2341 caccctatcc attctacatt catccagttc ctaccggcga cggtgcgaaa ttctccgagt
     2401 acccgtcgtg ctaaaatcta cattatactc acaatccgta tcgacggtta ataaataaaa
     2461 agtgaccttg tcattccgaa cgcagtgagg aatctaattt gttccctcct tacagtctgc
     2521 ctgccttttt tttccctcac tccacagagt taccccgcta ccgcgcgatt tttatcgtgt
     2581 ggttatatta ctttaaatat tttccacaca atacaattcc cgtaataaac gggacaagct
     2641 atgcggcagc ccgggcagat ttctcaatat cttaaacagt ttatcaattc aggcagttta
     2701 ttgggaattc tatttttctg aataatatct ttgtatctcc ccttcaactg attttccatg
     2761 acatttacaa tttcatctac actttcggga ttaaaaacaa tcggattttc aacaacctga
     2821 tgcgtaaaag gcaaatccga cgacagtatc tttaagccaa actgcaaacc ctcgataagg
     2881 ggcaacgcaa tggtttcagc ctgcgacgga aagatgagcg cacgggtaga ggagtaaatt
     2941 tcccgtactt catcgtaagg catatagcct ttattcacta ccaccgtgcg tttgtattta
     3001 cggtttatcg catctatttt ttcattcagt acattgcccg tatcgctgtc attgatggtc
     3061 atcactaagc gaaaggagta tttttccgac agcttatcca ccgcttccag caaccggttg
     3121 ttgtttttat gcggagcgcc cgaacccaca tagcaaaact catacactct ttctaaaggg
     3181 agcggagaag cttcacggta aaacggatac aactccgatt tcacctgcat attctcccac
     3241 aacgatttct gtaccgcttc ggtctggcaa gccactacat ccacattacg ggcaaaatac
     3301 ttaatccagt agaaatagac aaactttttc agattgaaaa ccaatgctgc cccttttacc
     3361 ggacgaggtg actcgaagaa aagtatatta tgtgcataca acaccgattt ttcacacttt
     3421 acaaaaggtg gcagattaca gaaaaacagc gtattacttc ttttccagaa ataacgcagg
     3481 aaagttttca gtccgtttgt tttgatcaac gaaatatttt tgtacttttt ctttccgaaa
     3541 tagtcaaata cctcctgata accaatataa acgttggctt ccagtgagtg ctgctcacaa
     3601 tactccagca actgctccag cagaacaaat ccgccacgga aatagatgtt ggaggcttca
     3661 atggtcagta acattaaagc atttttttta gacataacaa tttaaaaaac atcatataat
     3721 tcttcacaaa ac
//
LOCUS       DAOSDR010000322         3719 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrimonas sp. isolate AMR_MDS_1826
            NODE_8742_length_3719_cov_3.680950, whole genome shotgun sequence.
ACCESSION   DAOSDR010000322 DAOSDR010000000
VERSION     DAOSDR010000322.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863355
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrimonas sp. (wastewater metagenome)
  ORGANISM  Petrimonas sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Dysgonomonadaceae; Petrimonas.
REFERENCE   1  (bases 1 to 3719)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3719)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:10:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,720
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 35
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3719
                     /organism="Petrimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8742_length_3719_cov_3.680950"
                     /isolate="AMR_MDS_1826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2023866"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(122..661)
                     /gene="hpt"
                     /locus_tag="PLI30_11170"
     CDS             complement(122..661)
                     /gene="hpt"
                     /locus_tag="PLI30_11170"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019537756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HOI80149.1"
                     /translation="MNEIVTIKDKQFEKFIEFEQIQAAINRIAEKMDRDLRDKNPVFI
                     AVLNGAFMFAGELMKEVSVPCEITFVRLASYQGTTTTNKVQEVLGLNESIENRTVVIV
                     EDIVDSGNTMVALKEELNKLNPKEIKIATLLLKPDALKQEIQLDYVALEIPNDFIVGY
                     GLDYDGYGRNLKDIYKIRP"
     gene            860..2020
                     /locus_tag="PLI30_11175"
     CDS             860..2020
                     /locus_tag="PLI30_11175"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HOI80150.1"
                     /translation="MKKYVSLLPAVLLTAAVLLSCQSEKTFEVKGELSAAGDQTLYLE
                     HRGLGGVELLDSVKLKENGKFAFKEKAPVNPEFYQLRVGSQVAVFAIDSIETLQVRGD
                     AKDLASTLSIENSPVNEQIRQIDSQTRQVNIRISEAEKKHTAKAIDDVAYLAELDSVL
                     TEYKAEITKLILGNPSGAAAYYAVFQKVDGYLIFDPYNKQDYAMFGAVATSWDRYYPG
                     TPRSKHLYDFTMNALKTRKQQEQQAALLENVPVVSGLSLPDIVLTEVNGEKITLSSQK
                     GKVVVLDFTVYKSDFSPAHNMELNKIYSQHKAKGLEIYQISFDSDEHFWKNAASNLPW
                     ITVRDPQNVYSRLLSTYNVRDIPTAFVINRDGDVVARVENYATLPGEVGKVL"
     gene            2179..2649
                     /gene="greA"
                     /locus_tag="PLI30_11180"
     CDS             2179..2649
                     /gene="greA"
                     /locus_tag="PLI30_11180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008147156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HOI80151.1"
                     /translation="MAITYMTEEGLRKLKEELVHMESVQRPEISRQIAEARDKGDLSE
                     NAEYDAAKEAQGLLEGKIAQLKGLIATARIIDESAISTDEIQIMNKVTIKNLKTKKDM
                     TYMLVSESEADLKSGKIAVNTPIAQGLLGKKVGDKVDIQVPSGTLSFEVLDISI"
     gene            2654..3049
                     /locus_tag="PLI30_11185"
     CDS             2654..3049
                     /locus_tag="PLI30_11185"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009132533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HOI80152.1"
                     /translation="MATIFSRIIAGEIPSYKIAEDDRFFAFLDINPMAKGHTLVVPKQ
                     EIDYIFDLDDSLLAGMALFAKKVAAAIQAVIPCSRVGMMVIGLEVPHAHIHLIPIQKE
                     GDMSLANPRIRLSKEEFAEIAEKISSRIN"
     gene            complement(3068..>3719)
                     /gene="coaA"
                     /locus_tag="PLI30_11190"
     CDS             complement(3068..>3719)
                     /gene="coaA"
                     /locus_tag="PLI30_11190"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014225555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HOI80153.1"
                     /translation="KSTTARVLQKLLSILPEKPKVELITTDGFLYPNAELEKRGILNK
                     KGFPESYDAKNLLAFLAGVKSGRETLEAPVYSHLYYDIIPNEIQQIGKPDILIVEGIN
                     VLQVSLNKKPRRRLFVSDFFDFSIYVDAAEKDIRQWYLERFKTLQKTAFTNPESYFHQ
                     YASYREEELMKFANEVWEEINLPNLEQNILPTRFRADLILEKSEHHFVRGVRIRKI"
BASE COUNT          977 a          830 c          927 g          985 t
ORIGIN      
        1 cccgtttttg gtgttaccat taaattgata taaaaggaga cggcaaacga ttaatatatt
       61 tcaattttaa cgttttatcg ggaaattttt gaatcaggtt gtgacgactg atgcggtatt
      121 gtcagggcct aatcttgtaa atatctttca aattcctgcc gtaaccgtcg tagtccaggc
      181 catagcccac gataaaatcg ttggggattt ccagtgctac gtaatcgagt tgtatctcct
      241 gtttcagcgc gtccggcttc agtagcaggg tggcaatctt tatctctttg gggttgagct
      301 tgttgagttc ttctttgagc gccaccatcg tatttcccga atccacgatg tcttccacaa
      361 tcacaaccgt acggttctcg atactttcgt tcaaacccag cacttcctgt actttattgg
      421 tggtggttgt tccctgatag gaagccaatc gtacaaaagt tatctcgcag gggacgctca
      481 cttctttcat cagctctccg gcaaacataa aagcgccgtt gagtacggca ataaaaacag
      541 ggtttttatc gcgtagatca cggtccattt tttctgcaat ccgattgata gcggcttgaa
      601 tctgctcgaa ttcgatgaat ttttcaaact gcttgtcctt gatggttact atttcattca
      661 tgtgttgtgg attttaaaac cacaaaagta gcgctttttt tctttttctg taacaaaatt
      721 caacactttc tgtaattcgg cctgagttcc gggggcgggc aacctggatg tcccagcaga
      781 acaataaaat tgaacattct tccaaaattg acgaaaaatt gttaagtttg cgccctgaaa
      841 ttcttacgca atttttgata tgaaaaaata cgtttctttg ttacccgcag tgttgttaac
      901 agctgctgtc ctgctgtcct gtcagtcgga gaaaaccttt gaggtaaaag gagaactctc
      961 tgctgccggc gatcagacct tatacctgga gcaccgtggc ctgggtggtg ttgaactgct
     1021 cgattcggtt aagctgaaag agaacgggaa gtttgcattt aaagaaaagg cccccgtaaa
     1081 tcccgaattt taccagttga gggtagggag ccaggtggct gtttttgcca tcgactcgat
     1141 agagactttg caggtcaggg gcgatgcgaa agacctggcg agtacgttgt ccatcgaaaa
     1201 ctcgcccgtg aacgagcaga tcaggcagat cgattcccag acccgtcaag tcaacatccg
     1261 gatcagtgaa gcggaaaaaa aacatacagc caaggctatt gacgacgtgg cttacctggc
     1321 cgagctcgac tccgtcctga cagagtacaa ggcggaaatc accaaactga tactgggcaa
     1381 tccttcgggt gcggcggcat actatgcggt ttttcagaag gtggacggct acctgatatt
     1441 tgatccttac aacaagcagg attacgccat gtttggagcg gtggccacgt cgtgggaccg
     1501 gtactatccc ggcacccccc gatcgaagca cctgtacgac ttcacgatga atgccctgaa
     1561 aacaaggaaa cagcaggagc aacaggctgc cctgctcgag aatgttccgg ttgtttccgg
     1621 gttatcgctg ccggatatcg ttctgacgga agttaacggg gaaaaaataa ccctctcctc
     1681 gcaaaaggga aaagtggttg tactggactt caccgtgtat aaatcggact tctcccctgc
     1741 tcacaacatg gagttgaaca aaatctattc gcagcacaag gctaaagggt tggagatcta
     1801 tcagatctct ttcgattcag acgagcattt ctggaagaac gcggcgagca acctgccctg
     1861 gattaccgtt cgtgatccgc aaaacgtata ttcccgcctg ctctccacgt ataatgtccg
     1921 cgatatacct acggcatttg ttatcaacag ggatggtgat gtggtggcac gtgttgaaaa
     1981 ttacgcaacc cttcccggcg aagtggggaa agtattgtaa aatttggcaa aaagtttttt
     2041 taatttcgga gaaaggagta tctttgcagt aaacgtaaaa gattgaataa aggggattcc
     2101 ggtctgtaaa gcagccggaa ttctttttct ttaatctata tctgtgtcaa acatttaaaa
     2161 aataggagag ttaaaaaaat ggcaattact tatatgaccg aagaaggtct tcgaaagctg
     2221 aaagaggaac ttgtgcatat ggaatccgta caaaggccgg agatttcgcg tcagattgct
     2281 gaagcacggg acaagggcga tttatcggaa aatgcggaat acgatgccgc caaagaagcc
     2341 cagggattac tggagggaaa aatcgcacag ctgaaagggc tgattgccac tgcccgtatt
     2401 attgatgaga gtgctatcag caccgatgag attcagatca tgaacaaggt gaccatcaag
     2461 aacctgaaaa cgaaaaaaga catgacctat atgctggtgt cggaaagcga agcagacctg
     2521 aaaagcggca agatcgctgt gaatacgccc attgcacaag gcctgctggg taaaaaggta
     2581 ggagataagg tagatatcca ggtgccgtcg ggtacgctga gttttgaagt gttggatatt
     2641 tcgatttaat catatggcaa ctattttcag caggatcata gcgggagaga ttccctcata
     2701 caagatagcc gaggacgacc gttttttcgc ttttctggat atcaatccca tggcgaaggg
     2761 gcatacattg gtggtgccga aacaggagat cgattatatt tttgacctgg acgacagcct
     2821 gttggctggg atggcccttt ttgccaaaaa agtggctgca gctatccagg cggtgatccc
     2881 ttgcagccgc gtggggatga tggttatcgg gctggaggtc ccgcatgcgc acatacacct
     2941 gatccccatt cagaaggagg gagacatgag cctggccaat ccgcggattc ggctttcgaa
     3001 agaagagttt gcggagattg ccgaaaaaat aagcagccgg attaactgat tccccgcccc
     3061 gccggcctta tatcttccta atccttactc ccctcacgaa gtggtgctcg ctcttttcca
     3121 atatcaggtc ggcacggaaa cgggtgggga gaatgttttg ctccaggttg ggcaggttta
     3181 tctcctccca cacttcgttg gcaaacttca tcaactcttc ttcccggtac gaggcatatt
     3241 gatgaaaata ggattcggga ttggtaaatg ctgttttttg tagtgtcttg aaccgttcga
     3301 ggtaccattg ccggatatct ttttcagccg catccacata aatggagaaa tcaaaaaaat
     3361 cggagacgaa cagccgcctt cggggtttct tattgagaga tacctgcaag acgttgatgc
     3421 cttccacgat caggatatcg ggcttaccga tctgctgaat ctcgttggga ataatatcgt
     3481 aatacaggtg cgaatagacg ggagcttcca gcgtttccct tcccgacttc accccggcca
     3541 gaaaagcaag gaggttttta gcatcgtaac tttcggggaa tccctttttg tttaaaatcc
     3601 cccgcttctc aagttccgcg ttcgggtata ggaacccgtc ggtggtgatt aactctactt
     3661 tcggtttttc ggggagtatc gacaggagtt tctgcagcac ccgggcggtg gtgcttttg
//
LOCUS       DAOSDR010000323         3692 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrimonas sp. isolate AMR_MDS_1826
            NODE_8822_length_3692_cov_3.646962, whole genome shotgun sequence.
ACCESSION   DAOSDR010000323 DAOSDR010000000
VERSION     DAOSDR010000323.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863355
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrimonas sp. (wastewater metagenome)
  ORGANISM  Petrimonas sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Dysgonomonadaceae; Petrimonas.
REFERENCE   1  (bases 1 to 3692)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3692)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:10:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,720
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 35
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3692
                     /organism="Petrimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8822_length_3692_cov_3.646962"
                     /isolate="AMR_MDS_1826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2023866"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..869)
                     /locus_tag="PLI30_11195"
     CDS             complement(<1..869)
                     /locus_tag="PLI30_11195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005645846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA modification/repair radical SAM
                     protein"
                     /protein_id="HOI80154.1"
                     /translation="MNEKTLDKLKILADAAKYDVSCASSGTSRGSVKNGIGTAAGWGI
                     CHSFTPDGRCISLFKILLTNHCIYDCAYCSNRRSNDVVRSAFTAEELAELTIEFYRRN
                     YIEGLFLSSGVMRNPDYTMEQMLRVVRLLRETHRFNGYIHMKSIPGASRELIARAGTY
                     VDRMSVNLEIPSEQNLKLLAPEKDYESVFAPMRFIQQGMLESAEDRRKFRSAPKFVPA
                     GQSTQVIIGATPDSDKQILTLASALYKRPSFKRVYYSGYIPVNKNDNRLPIIATPPLV
                     RENRLYQADWLMR"
     gene            1091..1486
                     /locus_tag="PLI30_11200"
     CDS             1091..1486
                     /locus_tag="PLI30_11200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006800284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6249 domain-containing protein"
                     /protein_id="HOI80155.1"
                     /translation="MEELWVPIIAILCTIGLPVGFGMYLGLTSIRAEHKERMDLIRQG
                     IIPPERMKKKANPNRLVSLRNGIVLIALGIGVIVGFLCSENLTIGHDNEFWIIGASIV
                     FFLGLGYLTYFLVTQKMAIAQPREEPEQE"
     gene            1496..2053
                     /locus_tag="PLI30_11205"
     CDS             1496..2053
                     /locus_tag="PLI30_11205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019540001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HOI80156.1"
                     /translation="MNENERVQQVLSGKTSAFTYFVETYQDMAFTIAHRVCGNVQDAE
                     DVVQESFVKVYRNLHTFRNESKFSSWLYRIVYNTAITHSKSKLWLYDQETSLSNVQEI
                     SDFDTETQIIDSERTEAVANVLNKMSKGEALLLTLYYLEDNPVKEIAQITGLNESNVK
                     VKQFRARQSFKELYARYERVIPDLQ"
     gene            2068..2460
                     /locus_tag="PLI30_11210"
     CDS             2068..2460
                     /locus_tag="PLI30_11210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI80157.1"
                     /translation="MNEKNNIVHDDEEFIRGMMQDARMKAPENLKYRIMHQIETENAL
                     KPQQTPVRLSERKVLKDFVGIFGLMYAVLAALIGGAYLLKGKEFALSSGFLWTVILVA
                     FVFSFFWLMTRVDERLNDKRKGNHIPSK"
     gene            complement(2478..3350)
                     /locus_tag="PLI30_11215"
     CDS             complement(2478..3350)
                     /locus_tag="PLI30_11215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019540003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 2 family protein"
                     /protein_id="HOI80158.1"
                     /translation="MDFDYGKFKVKPGSKVNLKNFATSEDGGFDKKSARKEIENNIKQ
                     LKDLQEMFYADDRYSLLIILQARDAAGKDGVIRHVMSGINPQGCRVHSFKTPTKNELE
                     HDYMWRHYRALPERGMIEIFNRSHYENILATKVNPQWILNERIPGYSSVDEIDQQFWE
                     NRYAAVNAIEKHWHDNGMRILKFFLNVSKEEQKERFLARLDEPDKNWKFSSADLQARS
                     QWNKYDEAFEEMLEKTSKPYAPWYVIPADKKFFARVAVGDIILELFKSLDLHYPPAES
                     PEVLARAREQLMSE"
BASE COUNT         1035 a          847 c          820 g          990 t
ORIGIN      
        1 aaacgcatca accaatctgc ctggtagaga cggttttcac gaaccaaagg cggtgtcgcg
       61 atgatcggta gccggttgtc gtttttattt acaggaatat accctgaata atagacgcgt
      121 ttaaaagaag ggcgcttata cagggcagaa gccagcgtca gtatctgctt atcggagtcc
      181 ggcgtagcac caatgatgac ttgtgtactc tgcccagcag gcacaaactt aggggcagaa
      241 cgaaacttcc tgcggtcttc cgcgctttca agcatcccct gctgaataaa gcgcatgggc
      301 gcaaaaacac tttcgtaatc tttctcaggc gcaagcaatt tcagattctg ttccgatggg
      361 atctcgaggt tcacgctcat ccggtctaca tacgttcccg cccgggcaat gagttcccgg
      421 ctagcacccg gaatactctt catgtggata tatccgttaa aacgatgtgt ttcgcgcaac
      481 aacctcacca cacgaagcat ctgttccatc gtataatcgg ggttacgcat aacgcccgaa
      541 ctcaaaaaaa ggccttcgat gtaatttctc cggtaaaact caatagtcag ttcagccaat
      601 tcctcggctg taaaagcaga gcgcacgaca tcgttactgc gtcggttgga acagtatgcg
      661 cagtcataaa tacagtgatt ggtcaggagt atcttaaaca acgagatgca tcgcccgtcg
      721 ggagtgaagc tgtgacaaat gccccagccg gcggctgtgc ctatcccatt cttcacgctt
      781 ccacgggaag ttccgctcga tgcacacgac acatcgtact tggcggcatc ggcaaggatc
      841 ttcaatttat cgagcgtttt ttcgttcatg tcggggttgc ggattatgct ttacaaagaa
      901 aataaataga aatgaatccc acgaattcaa aatcataaaa atatttgtct tgttgataaa
      961 tatttcaaca acctgatttt caaacaaaaa ttaatcttat taaaaatatt tttctatttt
     1021 tgtaaccgaa tgttgttttc ctctgtctta tggatgtaaa caaattattc acttttaaaa
     1081 aattgtaatt atggaagaat tatgggtgcc aatcatagct attctctgta caatcgggtt
     1141 accggttggt ttcgggatgt atttggggtt aacctccatc agggcagagc acaaagaacg
     1201 aatggacctt attcgccagg ggataatccc cccggagcga atgaaaaaga aagccaatcc
     1261 caaccggctg gtatctctca gaaacgggat cgtacttatt gcactgggta ttggagtaat
     1321 cgtagggttt ctctgttcgg aaaacctgac tatcggtcat gataatgaat tctggatcat
     1381 tggagcaagc attgtcttct tcctcggttt gggatacctg acctatttcc tcgtgacgca
     1441 aaaaatggcg atagcccaac caagagaaga acccgaacag gaataattta ccgcgatgaa
     1501 cgagaatgag cgtgtacaac aggtgctttc gggcaaaact tccgcgttca cgtactttgt
     1561 cgaaacctac caggacatgg cgtttaccat cgcccaccgg gtttgcggca atgtacagga
     1621 tgcggaagac gttgtgcagg aaagtttcgt aaaggtatac cgcaacctgc acacgttcag
     1681 gaacgaaagt aaattctctt cgtggcttta ccgcattgta tacaacacgg caataacgca
     1741 ctctaaatca aaactatggt tgtacgacca ggagacgtca ctttcgaacg tccaggagat
     1801 ctcggatttc gataccgaaa cgcaaatcat cgattccgaa cgaaccgaag ccgtcgccaa
     1861 tgtgttgaac aagatgtcga aaggcgaagc gctgctgctt acgttgtact acctggaaga
     1921 caatccggta aaagagattg cccagatcac cggactgaat gaatccaacg tgaaggtgaa
     1981 acagttcagg gccaggcagt cattcaaaga gttatatgca agatatgaac gggtcatacc
     2041 cgatttacaa taaaaacgaa accgatcatg aacgaaaaga acaacatagt gcacgacgat
     2101 gaggagttta tccgcggaat gatgcaggat gcaagaatga aagcacctga aaacctgaaa
     2161 tacagaatta tgcatcagat tgaaaccgaa aacgccctca aaccccaaca aacaccggtg
     2221 agactaagcg aacggaaagt acttaaagat ttcgttggca ttttcggact gatgtatgcc
     2281 gttctggctg ctctaatcgg tggggcttac ctactcaagg gaaaagagtt tgccctctct
     2341 tccggatttc tctggacggt tattcttgtt gcttttgttt tcagcttctt ctggctgatg
     2401 acacgggtag atgaacgact gaatgacaag agaaaaggga atcatatccc ttcaaaatag
     2461 aaagaggccg gtccgatcta ttcgctcatt aattgctccc gtgccctggc gagcacttcg
     2521 ggtgactctg caggaggata gtgaaggtca agcgatttaa acaattccag aattatatcg
     2581 ccgacagcaa cacgggcaaa aaatttcttg tcggcaggaa tcacgtacca cggagcatag
     2641 ggtttcgacg ttttttccaa catctcttca aacgcttcat cgtacttatt ccattgcgag
     2701 cgggcctgca agtcggcaga actgaatttc cagtttttat ccggctcgtc aagccttgcg
     2761 aggaaacgct ccttttgttc ttctttcgat acgttgagga aaaactttaa aatgcgcatg
     2821 ccgttgtcgt gccagtgctt ttcaatagca ttgacagccg cgtaacggtt ttcccaaaat
     2881 tgttgatcaa tctcatctac agaactgtac ccggggatac gttcattgag aatccactgg
     2941 ggatttactt tggtggctaa tatgttctcg taatgtgagc ggttgaagat ctcaatcatc
     3001 ccccgctcag gcaacgccct gtagtggcgc cacatgtaat cgtgttccaa ctcgttcttc
     3061 gtgggagtct tgaagctgtg cacccggcat ccctggggat tgatgcccga catcacgtga
     3121 cggataaccc cgtcctttcc cgctgcgtcc cgcgcctgca aaatgattaa caacgaatag
     3181 cggtcatcgg cgtaaaacat ctcctgcagg tctttcagtt gcttgatgtt gttttcaatc
     3241 tcttttctgg cagatttctt atcgaacccg ccatcctcgg aggtggcaaa attcttcagg
     3301 ttcacttttg aaccgggctt taccttaaat ttaccgtaat caaaatccat tttttgggca
     3361 gtattagcgc ctaccggata tccggagact tcggttttta ttcaatatcc acaaagtaaa
     3421 taaaaaaaag aatgtaacgg caaaactcga cggaaaaaga taaatctgac tgtttgagaa
     3481 gtttgtcctt accagatgat agaaacaggc ggtttttatt tataaccgat tgcctgtttc
     3541 gtataaccag actgcggttg tcaaagctaa aattgatact atcggaattt tgatactgtt
     3601 tcctttcaat ttctcctact ttgctcatgg ttggatttat ttttaatctt caaataagtt
     3661 tatataatct tcaaacaggt aaatccttcc tc
//
LOCUS       DAOSDR010000324         3685 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrimonas sp. isolate AMR_MDS_1826
            NODE_8844_length_3685_cov_3.734435, whole genome shotgun sequence.
ACCESSION   DAOSDR010000324 DAOSDR010000000
VERSION     DAOSDR010000324.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863355
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrimonas sp. (wastewater metagenome)
  ORGANISM  Petrimonas sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Dysgonomonadaceae; Petrimonas.
REFERENCE   1  (bases 1 to 3685)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3685)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:10:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,720
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 35
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3685
                     /organism="Petrimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8844_length_3685_cov_3.734435"
                     /isolate="AMR_MDS_1826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2023866"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..426
                     /locus_tag="PLI30_11220"
     CDS             <1..426
                     /locus_tag="PLI30_11220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019538162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoPQ-activated protein PqaA family protein"
                     /protein_id="HOI80159.1"
                     /translation="NLLHYVPNAGHGLGDKIQAFGALSSFFAHMLYKTDYPTCEWNLT
                     GKGKNIDLKVKASPDNLVGATLWKSVSDSRDFRKATWTKSDIQLNAKNRSAVDIRIQY
                     PKKGFQAFYVDLVYKDPHGDEYTVSTRTFVSDNKKVFVE"
     gene            656..1573
                     /locus_tag="PLI30_11225"
     CDS             656..1573
                     /locus_tag="PLI30_11225"
                     /inference="COORDINATES: protein motif:HMM:NF016668.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HOI80160.1"
                     /translation="MRGILILGLLFFIGSLPAQTLAERLGYKKTDKLLIINNDDAGMC
                     HAANTATIEGMENGFISSATIMMPCSWSNEIVKYAKENPDKGFGVHLTLTSEWKNYRW
                     GTVAPRNEVPGLYDGQGYMWKGVREVYNASNTEEALIEGRAQIRKALDAGVRVTHIDS
                     HMGTYQYSPDYMYVYLQLAEEFNLPVRMPSQSTLDNMGAPPFRDICKEKGIIHPDYFI
                     HEELQNYKTENLEEFWTGYIKNIQPGVTEIYIHAATEGDEIRAITGSAPKRIKELEFF
                     TSDRFRQLIEKEGIIVISYHPLFELQRKK"
     gene            1570..>3685
                     /locus_tag="PLI30_11230"
     CDS             1570..>3685
                     /locus_tag="PLI30_11230"
                     /inference="COORDINATES: protein motif:HMM:NF016612.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="neutral/alkaline non-lysosomal ceramidase
                     N-terminal domain-containing protein"
                     /protein_id="HOI80161.1"
                     /translation="MKKIKIIGISLLVLLLLACIYSYTNMRDRHPGYSIDLKIESKEP
                     GVMRAGFAAVTITPEYMEPWNDMDNNARYEPKKGDTYEDLNGNGKFDTYWIAGFGNRV
                     AAQGVHDDLWARTMVLDDSNTRLAVVTVDVIGMFHPMVIDIRKMLPEEAGITYLVITS
                     THTHEAPDLLGLWGKSPFKSGVGKEWKEYIKKRVAQSVVEAVDALRPAHFRFSQNLTE
                     GMVTLKDTREPYVFDEGLRMMQVTDAETSQTLGTLIQWANHPETLWSKNLLISSDFPH
                     YLREAVEKGVYHGDSLVREGVGGVALYVNGALGGLMTTHASMEVHDPFRDTVYVEPSF
                     DKIRAQGDTLGLIILRTMQEEAVEVREAGINLRAKTFEIPLKNKLFLLAAAIGIMDAD
                     MTGWMKKRTEAAAWSIGPAGFITFPGELYPEILNGGVVALPGRDFPVDPLETPPLRDL
                     MQGEFRFGIGLANDEVGYIIPKSQWDVKEPYVYRDKAYYGEQNSLGPETAPLVYRELR
                     QLLEELPVTPPLPSVIEQARDALLERIISEIPAGKLNGLTHQQLLGMITEEEKEIFAN
                     DHWRFTVDNPALVSVMRHKGQEIVPFWLEEKGFHKTDMSVSNENYDYEVWQKEFPAGE
                     INLGINGFDLHRVVYFVTIGPVAGNQMPKILHHSPARWTVTPMEKGAYTYNDWDELVI
                     EQLPEELEGHILFTTIRGRAREA"
BASE COUNT         1042 a          783 c          978 g          882 t
ORIGIN      
        1 aatctgttgc attacgttcc caatgcaggc cacggtttgg gagataagat acaggctttt
       61 ggagcgctca gttcattttt cgcgcatatg ttgtacaaga cggattatcc tacctgtgag
      121 tggaacctga cggggaaagg taaaaatatt gacctgaagg taaaagcctc tcccgataac
      181 ttggtagggg ctaccctatg gaaatcggtc tcggacagca gggatttccg taaagctacc
      241 tggactaaaa gtgatataca gcttaatgcc aagaacaggt cggctgtcga cataaggatc
      301 caatatccga aaaaaggatt ccaagccttt tatgtggacc tggtctataa agatcctcat
      361 ggcgacgaat acacggtcag tacacggacc tttgtctcgg acaacaaaaa agtatttgta
      421 gagtaatggc tgatttttag aaactgtttt gatttttttc atcaattgtt ttttagtaat
      481 tttttgatgg atttggattt tcatttattg aaatgtagcg ggctacttga aagaaatgaa
      541 aattcgaagg cgcaaaaaag ggcaaaaaac aagatgaaag agtgaaaaaa taagtgtaga
      601 ataattactc gtaaaattca aaacagtttc ttaattaata aatagatcaa aagtgatgag
      661 aggaattctg atactgggtt tattgttttt tataggaagc ctgcccgccc aaacgctggc
      721 ggaacgcttg ggatataaaa aaacggataa actgctaatt atcaacaatg acgatgccgg
      781 tatgtgccat gctgccaata ccgctacgat tgaaggaatg gagaatgggt ttatcagctc
      841 ggctaccatc atgatgccct gttcctggtc gaatgagatc gtaaagtatg cgaaagaaaa
      901 tccggataaa ggatttggtg tgcacctcac attgacctcg gaatggaaga actaccgatg
      961 gggaaccgtg gcgccgcgca atgaagtccc ggggttatac gacgggcagg gctatatgtg
     1021 gaaaggtgtg agggaggtgt acaacgcctc caataccgaa gaagccttga ttgaagggag
     1081 ggcgcagatc cgtaaggcgt tggatgcagg ggtacgggtc acccatatcg actcccatat
     1141 gggaacctat cagtactcac cggattatat gtatgtctac ctgcaattgg cagaagagtt
     1201 taacctgccg gtgcgtatgc cgtcgcaatc cacactggac aatatgggag cacctccttt
     1261 cagggatatc tgtaaggaga aaggaattat tcatcccgat tactttattc acgaggagct
     1321 gcaaaactac aaaacagaaa atctggagga gttctggaca gggtatatta aaaatataca
     1381 gcccggagtg acggaaattt atattcatgc ggccacagag ggggacgaga tacgggcgat
     1441 caccggttct gctcccaaac gcataaagga attggaattt tttaccagtg accggttcag
     1501 gcaattgatt gaaaaagagg gtatcattgt tatcagttac catcccttgt ttgaattgca
     1561 aagaaaaaaa tgaaaaaaat aaaaatcata gggatctcgt tgctggtgtt gcttctcctg
     1621 gcctgtattt acagttacac caatatgcgc gaccgtcatc cgggatatag tatcgacctg
     1681 aaaatcgaaa gtaaggaacc gggagtcatg cgtgccggct ttgccgcggt tacgatcacg
     1741 cctgagtata tggaaccgtg gaacgatatg gacaacaatg cacgttatga gccgaagaaa
     1801 ggagatacct atgaggatct gaacggaaac gggaaattcg atacctactg gatcgccggt
     1861 ttcggaaacc gggttgctgc acagggtgtg catgacgact tgtgggcgcg gacaatggtg
     1921 ctggatgata gcaatacccg ccttgcagtg gtgacagtgg acgtgatcgg tatgtttcac
     1981 ccgatggtaa tcgatatccg gaaaatgctg ccggaagaag caggcatcac ctatctcgtg
     2041 attacatcca cccacaccca cgaagcccca gatctgctgg gactttgggg taagtcaccc
     2101 ttcaagagcg gtgtgggcaa ggagtggaaa gagtatataa agaaaagggt ggcccaatct
     2161 gtcgtggagg ccgtggatgc attgcgtcct gcccatttcc gtttttccca aaacctcacg
     2221 gaggggatgg tgacgctgaa agacacgcgt gaaccttatg tctttgacga aggcctcaga
     2281 atgatgcagg tcaccgatgc cgaaacctca caaacgcttg ggacactgat ccaatgggcg
     2341 aaccatcccg aaacattgtg gagtaaaaac ctgcttatct cctccgattt tcctcactac
     2401 ctgagggaag ccgtcgagaa aggagtctat cacggagaca gccttgtcag agagggggtc
     2461 ggcggcgttg ccctttatgt aaacggggca ctgggtggat taatgactac ccatgccagc
     2521 atggaggtac atgatccgtt ccgcgatacg gtgtatgtag agccgtcgtt cgataagata
     2581 cgtgcccagg gagataccct tggattgatc atactccgta cgatgcagga agaggctgtt
     2641 gaagtgagag aagcaggcat caacctacgt gcgaagacgt tcgaaattcc cctgaaaaat
     2701 aagctgttcc tccttgctgc cgctatcggg ataatggatg cggatatgac cggatggatg
     2761 aaaaagcgta ccgaagctgc tgcctggagc atcgggccgg ccggttttat cactttcccc
     2821 ggagaactct atccggagat attgaacgga ggggtagtag cccttcccgg ccgggatttt
     2881 ccggtcgatc ccctggaaac gccacctctg cgtgacctga tgcagggaga gttccgtttt
     2941 ggcatcggtc ttgccaatga cgaggtcgga tatataattc ccaaaagcca gtgggatgtg
     3001 aaggagccgt atgtataccg ggacaaggct tactacggtg aacagaattc gctcggtccc
     3061 gagaccgctc cacttgtata tagagaattg cgccaactcc tggaagagtt acctgttact
     3121 cccccactcc cttctgtgat cgaacaggcg agggatgcac tgttggaacg gattatttct
     3181 gaaattccgg cagggaaatt gaatgggttg acccatcagc aactattggg aatgatcact
     3241 gaagaggaaa aagagatctt cgccaatgat cattggcgtt ttacagttga taatcctgcc
     3301 ctggtgtcgg taatgcgtca taaagggcag gaaatcgttc ctttctggct ggaggaaaag
     3361 ggattccaca agaccgatat gtcggtatcc aacgaaaatt acgactatga agtatggcaa
     3421 aaggagtttc ctgccggaga gattaacctc gggatcaacg gatttgacct gcaccgggtg
     3481 gtctattttg tgacgatcgg tccggttgcc ggaaatcaga tgccaaaaat tttacatcat
     3541 tcccccgcca gatggacagt gaccccgatg gaaaaaggag cttataccta taacgactgg
     3601 gatgaattgg tgatcgaaca gctacccgaa gagcttgaag ggcatattct ctttaccact
     3661 atacggggac gtgcacgtga agccg
//
LOCUS       DAOSDR010000325         3682 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrimonas sp. isolate AMR_MDS_1826
            NODE_8856_length_3682_cov_3.897436, whole genome shotgun sequence.
ACCESSION   DAOSDR010000325 DAOSDR010000000
VERSION     DAOSDR010000325.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863355
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrimonas sp. (wastewater metagenome)
  ORGANISM  Petrimonas sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Dysgonomonadaceae; Petrimonas.
REFERENCE   1  (bases 1 to 3682)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3682)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:10:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,720
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 35
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3682
                     /organism="Petrimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8856_length_3682_cov_3.897436"
                     /isolate="AMR_MDS_1826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2023866"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..622
                     /locus_tag="PLI30_11235"
     CDS             <1..622
                     /locus_tag="PLI30_11235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019539154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF853 family protein"
                     /protein_id="HOI80162.1"
                     /translation="NPADIPPVILGQLGNRVQHALRAYTPNDQKAVKVAANTFRSNPK
                     FDTEEAITQLNTGEALVSFLDEKGAPQMVQRANILPPQGQIGPITAGERDQIMKQSLI
                     YGVYEKLVDRESAFEILSQKQELLAEEREQAEAEKERIRLEKEERRLQAEAERERRAE
                     ARRKKEERGIVGDLLEQVGRSATRQISSQLGRTITRSIFGAFFGKK"
     gene            643..2124
                     /locus_tag="PLI30_11240"
     CDS             643..2124
                     /locus_tag="PLI30_11240"
                     /inference="COORDINATES: protein motif:HMM:NF039827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5687 family protein"
                     /protein_id="HOI80163.1"
                     /translation="MYSTFFKHYWLKSVRAPGYYKNLIVNIFVGLSAVYFLVIFVLLG
                     FMMPRILAEAAPKLDPALTFNGILMYVTVLALLFRFLFQPLSTINLQSYQVLPVKRSK
                     LVNYLLIKPLLNPANYLTLCFAIPFSVTGIHPAYGLGGAFRFVLIVIFLIWFNTLFAS
                     FLKRKFGSSITGIASFLLIVAGLAALEYFNIYSLFALSQQVFGFLIENPIGWIIVLVF
                     SGAAFLLNKGFFAQNYYPENFDRKTIRKQAETQNFTFMERFGKIGEIISLEMKLVLRH
                     KRTKNVLYTAVLFLLYGLLFYPSDMYKENDAMLMFVAIFVTGIGMILFGQWIINWEGS
                     YFDFLMTRDIDTQSYIKANYFLLLSLSIISFILTTPYFLFGRDILINHSVAFLYNAGV
                     NIHVYLFGATFNTKRLELSKGSAMNMQGVSYKNFIIMIPLLVVPMGLIGIFSLFSAKH
                     IALIILAVLGLAGIVFWKQLLEITEKQFLRRKYALCEGFRKKE"
     gene            2158..2931
                     /locus_tag="PLI30_11245"
     CDS             2158..2931
                     /locus_tag="PLI30_11245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019539151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOI80164.1"
                     /translation="MNAEEIQPTLDTAISMPPPVPIAANTVIKVTDLKKIYNGVAVLN
                     IPEITVKNGESFGLVGNNGAGKTTFFRLILDLIEASSGEVRIDGERVARRDDWKSKVG
                     SFLDESFLIDFLTPEEYFAFTGKVYGKSEGDITAFLESMKEFFNGEILGSKKLIRDLS
                     KGNQKKTGIAAALLSDPQILVLDEPFTALDPTSQIRLKRMLNELKSTRSMTMLISSHD
                     LNHVTEVCDRIVVLEKGLVVRDTQTTQDTLKELESYFAV"
     gene            complement(2937..>3682)
                     /locus_tag="PLI30_11250"
     CDS             complement(2937..>3682)
                     /locus_tag="PLI30_11250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019540429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="neutral zinc metallopeptidase"
                     /protein_id="HOI80165.1"
                     /translation="KNPFQAIDMVNSVVPGQTSEEVVDPSRMNENEDLKVFTLGVFNS
                     ANDVWTEIFRTQMGESYRNPVLVNFTDQTTSACGGATAQVGPFYCPGDEKVYIDLNFF
                     HQLTRDYGARGDMAMAYVTAHEVGHHVQKLLGIVDQVNQYRGRISEAEYNKIMVKMEL
                     QADFFAGLWANQASKLGIIQLEPGDLESAISATTAVGDDTLQKEAMGYTVPDSFTHGT
                     AAQRTYWFRKGYQTGDIRQGDTFNDPSLR"
BASE COUNT          957 a          942 c          878 g          905 t
ORIGIN      
        1 aaatcccgcc gatatcccgc ccgtaattct cggacaactc ggcaaccgtg tgcaacacgc
       61 gctccgagcc tacacgccca acgatcagaa agcggtaaag gtggcggcca acacgttccg
      121 gtccaatccg aagttcgaca cggaagaggc tatcacacaa ctgaacaccg gcgaagcgct
      181 ggtgtcgttt ctcgacgaaa aaggtgcgcc ccaaatggtg caacgcgcca atatcctgcc
      241 gccgcagggc cagatcggac cgattacggc aggggaaagg gaccagataa tgaagcaatc
      301 actcatttat ggagtgtatg aaaaactggt cgaccgggaa tccgctttcg aaattctttc
      361 ccaaaaacag gaactgctcg ccgaagaacg tgagcaggca gaagccgaga aagagcgtat
      421 ccgtctggaa aaagaggaac ggaggttaca ggcggaagcc gaacgtgaaa gacgcgccga
      481 agcccgcagg aaaaaagaag agcggggcat cgtgggcgat ttgctggagc aagtgggcag
      541 aagtgccaca cggcaaataa gttcgcaact cggcagaacc atcacccgaa gcattttcgg
      601 cgcatttttc ggaaaaaaat aaaagcatca gactaaaact tcatgtattc aacatttttc
      661 aaacactatt ggctgaaaag tgtacgcgca cccggatatt ataaaaacct gatcgttaat
      721 atattcgtcg ggttgagcgc tgtatacttc ctggtaatct tcgtactcct gggttttatg
      781 atgcccagaa tcctggcaga agccgctccg aaactcgatc ccgcactcac gtttaacggt
      841 atcctgatgt acgtaaccgt cctggcattg cttttccggt tcctgtttca accgttgagc
      901 accatcaacc tgcaaagcta tcaggtgttg ccggtgaaga gaagcaagct ggtgaattac
      961 ctgttgatca aacccttgct caacccggca aactacctca cactttgttt tgccatacct
     1021 ttttccgtca cgggtattca cccggcatac ggtttaggcg gagcgttccg gtttgttctg
     1081 atcgtcattt tcctgatctg gttcaacacg ctttttgctt ctttcctgaa acgtaaattc
     1141 gggtcatcca ttacgggaat cgcctctttt ctcctgatcg tcgccggact ggcagcattg
     1201 gaatatttca acatctactc ccttttcgcg ttatcgcagc aggtgttcgg cttccttatc
     1261 gaaaatccga ttggatggat aattgttttg gtattcagcg gagcagcttt cctgctgaac
     1321 aaagggttct tcgctcagaa ttattatccc gagaacttcg acagaaaaac aatccggaag
     1381 caggccgaaa cccaaaactt cacgttcatg gaacgttttg ggaaaatagg agagatcatt
     1441 tccctggaga tgaaactcgt gttaaggcat aaacggacca agaacgtgct ctacacagct
     1501 gtacttttcc tgctctacgg gttgctgttt taccccagcg acatgtacaa ggaaaacgat
     1561 gccatgctga tgttcgtcgc tatcttcgtt accggcatag gcatgatcct gttcggacaa
     1621 tggatcatca actgggaagg ttcctatttc gatttcctga tgacaagaga catcgatacc
     1681 cagtcgtaca tcaaggccaa ttattttcta ctgttgtcgc tcagcatcat ctccttcata
     1741 cttaccacgc cctatttcct gtttggaagg gatattctga tcaatcactc ggttgctttc
     1801 ctgtataacg caggggtgaa catccacgtc tacctctttg gggccacgtt caataccaaa
     1861 cggttggaac tctcaaaagg ctctgccatg aacatgcagg gagtgagcta caaaaacttc
     1921 atcatcatga ttcccctgct ggttgttccg atgggattaa tcggtatttt ctcgttgttt
     1981 tccgctaaac acattgctct catcatcttg gccgtcctgg ggctggccgg cattgttttc
     2041 tggaaacaac tgctcgaaat taccgaaaag cagttcttga ggcggaaata cgcgctttgc
     2101 gaaggattca gaaagaagga atgaacttac cacgaacaaa taattaaaat taacgatatg
     2161 aacgctgaag aaattcaacc aaccctggat accgcaattt caatgccgcc acccgtaccc
     2221 attgcagcaa atactgtgat taaagtaacc gatctcaaga aaatatacaa cggcgtagct
     2281 gttttaaata ttccggaaat aaccgttaag aacggcgaaa gcttcggact ggttggaaac
     2341 aacggcgcag gaaaaaccac ttttttccgg ctgatactcg acctgattga agcgagctcg
     2401 ggcgaagtac gtattgacgg ggaaagagtt gcccgtcgcg acgactggaa atcgaaagtg
     2461 gggtcgttcc tcgacgagag tttcctgatc gactttctta ctcccgagga atattttgct
     2521 ttcacgggaa aagtctacgg aaaatcggaa ggcgatatta ccgccttttt agagtcgatg
     2581 aaggagttct ttaacggcga gatcttgggt tcgaagaagc tgatccgcga cctctcgaaa
     2641 gggaaccaga aaaagaccgg gatagccgcc gcacttctca gcgatccgca gatacttgtt
     2701 ctcgatgagc cgttcaccgc gctcgacccc acttcgcaaa tccgcctgaa acggatgctc
     2761 aatgaactga aaagtaccag gagcatgacg atgcttattt cgagccacga cctcaaccac
     2821 gttacggaag tctgtgaccg cattgttgtt ctcgagaaag ggctggtggt gagggatacc
     2881 caaaccaccc aggacaccct gaaagaattg gaaagttatt ttgccgtgta acggatctat
     2941 ctgaggctcg gatcgttgaa cgtgtcacct tgccggatat cgccggtctg gtaacctttg
     3001 cggaaccagt atgtccgttg ggcagccgtt ccgtgcgtga atgaatcagg gacggtgtaa
     3061 cccatcgcct ccttttgcag ggtatcatca ccaacggcgg tcgtggcgct gatggccgac
     3121 tccaagtctc ccggctcgag ttgaattatt cccagtttgc tggcctgatt ggcccacaac
     3181 ccggcgaaaa aatcggcctg cagttccatc ttgaccatga tcttgttgta ttcagcttca
     3241 ctgatacggc cccggtattg gtttacctga tcgacaatac ccagcagttt ctgcacgtga
     3301 tgccccactt catgagccgt aacgtaagcc attgccatgt cgcccctagc accgtaatcg
     3361 cgtgtcaact ggtgaaagaa gttgagatcg atatacactt tttcatcgcc gggacagtaa
     3421 aaaggcccca cctgagccgt tgcgccgccg caggcagagg tggtctgatc ggtaaaattc
     3481 acaagaaccg ggttacggta actttcaccc atctgagtcc tgaaaatttc cgtccacaca
     3541 tcgttggcgc tgttaaaaac accaagcgtg aatactttca ggtcttcgtt ttcgttcatg
     3601 cgcgaaggat ccaccacctc ttccgatgtt tgtccgggca ccacactgtt taccatatcg
     3661 atggcctgaa aagggttctt cc
//
LOCUS       DAOSDR010000326         3670 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrimonas sp. isolate AMR_MDS_1826
            NODE_8883_length_3670_cov_2.484371, whole genome shotgun sequence.
ACCESSION   DAOSDR010000326 DAOSDR010000000
VERSION     DAOSDR010000326.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863355
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrimonas sp. (wastewater metagenome)
  ORGANISM  Petrimonas sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Dysgonomonadaceae; Petrimonas.
REFERENCE   1  (bases 1 to 3670)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3670)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:10:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,720
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 35
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3670
                     /organism="Petrimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8883_length_3670_cov_2.484371"
                     /isolate="AMR_MDS_1826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2023866"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..295)
                     /locus_tag="PLI30_11255"
     CDS             complement(<1..295)
                     /locus_tag="PLI30_11255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006799138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HOI80166.1"
                     /translation="MKEKAKLSTSINLKLTEADFNKIKDKAEKLGMKATQYVREMVLK
                     GSVKSRFTLEELDLMRKLAGIANNLNQIAKKANQAGYIMEAIEIMKIMNQIKRM"
     gene            420..641
                     /locus_tag="PLI30_11260"
     CDS             420..641
                     /locus_tag="PLI30_11260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI80167.1"
                     /translation="MKRRLAGQSQSNETIGSIAKGLSSDLEPADHRELVLKVAAPQGK
                     VCTGVTGKDPQGFLGYPCDYLAPEHPMFR"
     gene            complement(690..1253)
                     /locus_tag="PLI30_11265"
     CDS             complement(690..1253)
                     /locus_tag="PLI30_11265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006844232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3408 domain-containing protein"
                     /protein_id="HOI80168.1"
                     /translation="MERKEANNAMQRKGSEKKQVPDDFNPLMPGSVKRRTPQDADSSI
                     KKFLSNDLIDSFSKDADDASGTNDSVYTNDTVNTTDTQKTIEADETVSSEPKVSTTPI
                     VPTVPKRISSKHRKESLDEYRDTFLQVPKLDDRKPVFVSREVRDRLDEIVRKLGGRRL
                     SVSGLIENLARHHLSAYHEDIEVWRKL"
     gene            1609..1923
                     /locus_tag="PLI30_11270"
     CDS             1609..1923
                     /locus_tag="PLI30_11270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006844230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOI80169.1"
                     /translation="MDIVNIEAKTFEAMLTKFESFAARMEHLCRLHSDKDMKEWLDNQ
                     DVCLLLNISPRTLQTFRDNGTLAYSQINHKIYYKPADVERILPLVEKKRKQVAYKTGR
                     KI"
     gene            1942..2241
                     /locus_tag="PLI30_11275"
     CDS             1942..2241
                     /locus_tag="PLI30_11275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002561205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOI80170.1"
                     /translation="MSNELITSENNERVKSFFLSLDRLASAMERLFVSRKPTLNGESF
                     YTDEELSKKLKLSRRSLQDYRNEGRIPYIKLGGKILYRASDIEKLLEEGYRDSWK"
     gene            complement(2276..3523)
                     /locus_tag="PLI30_11280"
     CDS             complement(2276..3523)
                     /locus_tag="PLI30_11280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010538624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HOI80171.1"
                     /translation="MNVIKSTFNLLFVIRKHRLLKNGEAPIYLRVTVNGEVADITTKR
                     SIHVKMWDQRRESSSGKTVKDRELNHYLETLKMRMYQIHRTFEIDGRQITAKALRDSF
                     YGRDENDKTIVDVYREHNKKCRALIGIDFTKSTVEKFDTSLSHLQEYIRHQYGKDDML
                     LSEINGQLVSDFDFYLKTVRKCQNNSSIKHLKNLKKVIRIALANDWMKKDPFFGIQFK
                     HDETHVEFLTQEELGRIMNKEFTLPRLALVRDVFVFCAFTGLAFIDVKNLTSEHLMKD
                     NNGALWIRKPRQKTGNMSNIPVLSVTKGLIEKYANHPECLKKNVLLPVMSNQKLNAYL
                     KEIADLCGIKKKLSTHVARHTAATVVFLANNVSMENVAKILGHSNTKMTQHYAKVLDQ
                     SIMRDMADVEKCFGGLSVEKGKE"
BASE COUNT         1042 a          792 c          713 g         1123 t
ORIGIN      
        1 gcatcctttt gatttggttc ataattttca ttatctcaat cgcttccata atatatccag
       61 cctgatttgc ctttttagca atctgattca gattgtttgc tatccctgcc aacttacgca
      121 tcaggtcgag ttcttctaac gtaaaacgag atttaacgct ccctttaagt accatttccc
      181 gaacatattg tgtggctttc atacctaatt tttccgcttt atcctttatt ttattgaaat
      241 ccgcctcggt caatttgaga ttaatcgaag tggatagttt tgccttttct ttcacaggac
      301 gacctcgttt tcgcctattt ctctctctgt ttcccataac atttatcctt taatggttat
      361 ggaatctttc gggctatgca aatagccgtg cgagccaaag aggaagcgac caacgggaga
      421 tgaaacgcag acttgcagga caatcccaaa gcaatgagac catcgggagc atcgcaaagg
      481 gattgtcaag cgaccttgaa cctgccgacc atcgggagct ggttttaaag gtcgcagccc
      541 cgcagggcaa ggtatgcaca ggagtaaccg gaaaagaccc tcagggattt ttgggttacc
      601 cgtgcgatta ccttgctccc gaacatccca tgttccggta gaaaagataa gctgcaaaat
      661 atcacagctt atcctttcgt aaccggaggt tacaactttc gccatacctc gatatcttca
      721 tggtatgcgc ttaggtgatg acgagctaaa ttctctatca agcccgacac actcaatcgt
      781 cgtccaccta atttacggac gatctcatcc aacctgtccc gcacctcacg gctgacaaag
      841 accggcttgc ggtcgtccag cttcggaact tgcaagaagg tgtcacgata ttcatccaat
      901 gattctttcc gatgtttgct actgatacgc ttgggcacag tcggaactat tggtgtggta
      961 gatacttttg gttctgaaga cacagtttca tccgcttcaa tcgttttttg agtatcggtt
     1021 gtgttgacag tatcattagt ataaacacta tcgtttgtac cacttgcatc atctgcatct
     1081 ttactaaagc tatctatcaa atcatttgag agaaattttt taattgagga atcagcgtct
     1141 tggggtgttc ttcgttttac gctccctggc atcaggggat taaaatcatc aggtacttgt
     1201 ttcttctcac taccttttct ttgcattgca ttgttcgctt cttttctttc catacttttc
     1261 agtcttttat aagtgaatat tacggctgct gtatatgcag taaaccgatt gtcgcttgca
     1321 aagaaagatc tattaatcag gattgtattt acactggatt catatgaaat attctgcaat
     1381 atatttcaat attggtctca ttgcagaggg tacaaatttc aacattatgc agtgtagaca
     1441 agaatcagtc ctactaatct ccctattcga gatactttgt ctatcaaatg ttggcttcat
     1501 acatagtcaa atcgacgcca cacacttcca tctcattacc aactcattgt gatatgatat
     1561 tttcttcttt ccttcgtcaa aaaatatcaa gaacaaaaac taaaacatat ggatatcgta
     1621 aatattgaag ctaaaacatt cgaggcgatg cttacgaaat tcgagagctt tgcagccaga
     1681 atggaacatc tctgccgctt acatagcgac aaggacatga aagagtggtt ggataatcag
     1741 gacgtgtgcc tacttctgaa tatatctccg agaacgttac agacatttcg ggataatgga
     1801 acgctggcct attcccaaat caatcacaag atttattaca aacctgccga tgtggaacgt
     1861 attctaccac tcgtggaaaa aaagcggaaa caggtggcat ataaaacagg gaggaagata
     1921 taaccaatta aaaacaagaa tatgagcaat gagttaatta catcagagaa caatgaaagg
     1981 gtaaaatcat tttttctttc attggatcgt cttgcgtcgg caatggaacg cctattcgtc
     2041 agtcgcaagc cgacattgaa cggagagagt ttctatacag acgaagagtt atcaaagaaa
     2101 ttgaaattaa gccggagaag tctccaagac tacagaaatg aaggacgtat cccttatatc
     2161 aagttgggag gaaaaatttt gtatcgggct tcggatatag agaaattgct ggaagaaggg
     2221 taccgggata gctggaaata actgactgtc tccaaactac cccttacctc cttgattatt
     2281 cttttccttt ttcgactgat aaaccaccaa agcatttttc cacatcagcc atatcgcgca
     2341 taattgactg gtcgagaacc ttggcgtaat gctgggtcat tttcgtattg gagtggccga
     2401 gtatcttggc aacattctcc atcgacacat tattggctaa gaaaacaacg gtagctgcgg
     2461 tatgtcgggc tacgtgagtg ctaagtttct tcttgattcc gcacaaatct gcaatttcct
     2521 tcagataggc attgagtttt tgattgctca ttacaggcag taacacattc ttcttcaagc
     2581 attcagggtg attggcatat ttttctatca atccctttgt aacagacaat accggaatat
     2641 tgctcatatt cccggtcttc tgccgtggct tccgaatcca taatgctccg ttattgtctt
     2701 tcatcagatg ttccgacgtg agattcttga catctataaa tgccagaccg gtgaaagcac
     2761 aaaacacgaa aacatccctc acaagggcga gccgtggcag agtgaactcc ttattcatga
     2821 ttcgtcccaa ttcttcctgc gtaagaaatt ccacatgggt ttcatcatgt ttgaattgga
     2881 ttccaaagaa agggtctttt ttcatccaat cgttagccaa agcaatacgg atgacttttt
     2941 tcaggttttt caaatgcttg atactggaat tattctggca cttacgaacg gttttcagat
     3001 agaaatcgaa atcgcttaca agctgaccgt tgatctccga taagagcata tcatctttgc
     3061 catattggtg tcgaatgtat tcctgaagat gggataacga ggtgtcgaat ttctcaacgg
     3121 tagatttagt aaaatcaata ccaatcaatg cccggcattt tttgttatgc tcccgataaa
     3181 catctacaat ggttttatca ttttcatccc gaccataaaa gctatcacgc aaagctttcg
     3241 ctgttatctg cctgccgtca atctcaaagg tacggtggat ttgatacatc cgcattttga
     3301 gcgtttcgag atagtgattc aactcacggt cttttaccgt ctttccggaa ctggattccc
     3361 gtcgttgatc ccacatcttg acatggatac tccgtttagt ggtaatatcg gcaacctctc
     3421 cgtttaccgt aacacgtaaa taaatagggg cttctccatt tttaagaagc ctgtgctttc
     3481 tgattacaaa aagcaaattg aacgttgatt tgattacatt catgggcttt aaaattttgt
     3541 tttgcaaagt tactttatat agcccattaa caagataagc aaattattga aaatcaacga
     3601 ataaaaaccc aaatcgtggg caaaaaatca ggattaaaaa agcccacgaa ttgtgctgtg
     3661 aatagtgcgt
//
LOCUS       DAOSDR010000327         3633 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrimonas sp. isolate AMR_MDS_1826
            NODE_8999_length_3633_cov_4.455841, whole genome shotgun sequence.
ACCESSION   DAOSDR010000327 DAOSDR010000000
VERSION     DAOSDR010000327.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863355
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrimonas sp. (wastewater metagenome)
  ORGANISM  Petrimonas sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Dysgonomonadaceae; Petrimonas.
REFERENCE   1  (bases 1 to 3633)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3633)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:10:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,720
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 35
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3633
                     /organism="Petrimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8999_length_3633_cov_4.455841"
                     /isolate="AMR_MDS_1826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2023866"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1358)
                     /locus_tag="PLI30_11285"
     CDS             complement(<1..1358)
                     /locus_tag="PLI30_11285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011966593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HOI80172.1"
                     /translation="MLNKIIKYFLNNRFITILLLITIIVWGISVAPFNWGSILPKDPV
                     PVDAIPDLSDNQQIVATEWMGRSPKDIQEQITYPLTTSLLGVPGVKTIRSTSMFGMSF
                     IYIIFEENIEFYWSRSRILEKLNSLPPGTLPQGVQPSLGPDATALGQIFWYTLEGRNP
                     ETGKPSGGWDPDELRTVQDFYIRYSLMSAEGVAEVASVGGFVKEYQVDVNPNSLRAYN
                     LTIMDVMNAIQKSNLDIGAETVEINKAEYLVRGLGYIKKVSDLEEAVVTVRDGIPVKI
                     KDLAFVTLGPATRRGGLDKEGVEAVGGVVVARHGSNPLQVIDNVKETIKEMDAGLPQK
                     TLADGSVSKVTVVPFYDRTALIHETIGTLETSLTHETLIAIIVVIVLVLNLRASVVIS
                     SILPVGVLATFIIMKYAGIQANIVALSGIAIAIGIMVDVGVVFVENTLRHMDMEENKG
                     IR"
     gene            complement(1516..1962)
                     /locus_tag="PLI30_11290"
     CDS             complement(1516..1962)
                     /locus_tag="PLI30_11290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI80173.1"
                     /translation="MALFLLNIMALVAVQPVIAMHFCEGELYSWGLFASNDESSCCQP
                     GTKGHSCCGTHSSDNPNCSLNKALDDCCDFETIQVATDDYQKQIQEFNSDKLSLTFEN
                     VWLTLYNLLGGTIAEPNTLLLQNDFPPKGLFLQDVSIRDYICIYRI"
     gene            2191..3126
                     /locus_tag="PLI30_11295"
     CDS             2191..3126
                     /locus_tag="PLI30_11295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019537734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HOI80174.1"
                     /translation="MKTRSLKKFMYLSIAAAVVTIGLKFTAYLKTGSMGFFSDALESF
                     VNLFAAVIALILLHISEKPADEGHEYGHGKAEYFSSAIEGALILIAAFSIIYTSIPRI
                     IHPQPLENLGIGTLFSVGASLVNLFAGTLLIQNGKKHRSMVLEADGKHLMTDVWTSAG
                     VIAGVLVVKFTGLLVLDPIIAILVALNIVYTGYKLISRSASGLMDASIPQEEIKQITG
                     YFDSLKGKNIDYHSLLTRQAGHRKFISFHLLVPGDWTVKEGHDYAEIIEQRIENMFDD
                     TVTVTTHIEPIEDPLSLNDIKIDRMNYSDDDDFDT"
     gene            complement(3127..>3633)
                     /locus_tag="PLI30_11300"
     CDS             complement(3127..>3633)
                     /locus_tag="PLI30_11300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI80175.1"
                     /translation="TVEDNGNYTLTDGTNQYVITLEPVTGNKIFMVKDGFRWMQLNYF
                     VKSSDKPSSPVSISTDFLSRSELLSYREKIRIDKNTYKNKFRNGFYQSDFNPEFTFRA
                     HDDGTYSIRFYSLELSNGTWEKEDNFLKLKDDNLAAYFFVAVEPEDKLKSILMPGDFS
                     LTRFSKVS"
BASE COUNT         1004 a          825 c          767 g         1037 t
ORIGIN      
        1 tttctaatgc ccttattctc ttccatgtcc atgtgtctga gcgtattctc gacaaatacc
       61 actcccacat caaccatgat tccgattgcg attgcaatac ccgatagtgc tactatgttg
      121 gcttgtattc ccgcatattt cattatgata aaagtggcca gtactcccac aggtaaaatg
      181 ctggaaataa caaccgaagc cctgaggttt aagaccaaaa caattacaac aatgatggcg
      241 ataagcgttt catgggtaag cgaagtctcc agcgtaccga tcgtttcgtg aatcagcgca
      301 gttctgtcgt aaaaaggaac aacggtcact ttagaaacag atccatctgc aagtgtcttt
      361 tggggtaatc ctgcgtccat ctctttgatt gtctccttca cgttgtcgat aacctgaagc
      421 ggattggaac catggcgtgc caccaccaca ccacccacgg cctctacccc ttccttgtcg
      481 agaccgcccc gacgtgtagc aggaccaagg gtgacgaatg ccaaatcttt aatctttacc
      541 ggaataccat ctcttacggt aaccactgct tcttccaggt cggatacttt ttttatgtaa
      601 ccaagtccgc gaacaaggta ttccgcttta ttgatttcaa cagtttcggc accgatatcg
      661 agattgctct tctgtatggc gttcattaca tccataatag tgaggttgta tgcgcgcaga
      721 gaattgggat ttacatctac ctggtactct ttcacgaaac cacccaccga agccacctca
      781 gccactcctt ctgccgacat taacgagtat ctgatataga aatcctgcac tgtcctcaat
      841 tcatccggat cccagccacc tgaaggttta cctgtttcag ggtttctccc ttccagggtg
      901 taccagaaga tttgtcccaa agcagttgca tccggcccca ggctcggttg tacgccctga
      961 ggcaatgtgc cgggaggcaa tgagttcaat ttttccagaa tccgcgagcg gctccagtaa
     1021 aattcaatat tttcttcgaa gatgatgtag ataaaagaca tcccaaacat ggatgtgctt
     1081 cggatggttt taaccccggg gacacccagt aatgaagtag tcagcgggta ggtaatctgc
     1141 tcttgtatat ctttgggaga acgtcccatc cattctgtcg caacaatctg ttgattgtca
     1201 ctgagatcgg ggattgcatc tacaggaacc ggatcctttg gcagaatgct accccagtta
     1261 aaaggtgcaa ctgatattcc ccatacgata atagttataa gtaacaggat ggtaatgaat
     1321 ctgttattca ggaaatactt gattattttg tttagcataa tgattctatt cgattgttga
     1381 acaacttatc tgtttgaaaa taaaagcatg cattgtattt tttcaacttc aatccttgtc
     1441 ggtatgaaaa catgaaaaat gcacataagt gtcctgtcaa taaccggtaa ttgcgatcat
     1501 tgacaatcga aaaaatcaaa tgcggtagat gcagatgtaa tcgcggatac ttacatcttg
     1561 cagaaaaagc cctttgggcg gaaagtcatt ttgaagtaaa agggtattgg gttcggcaat
     1621 tgtcccccca agcaggttat acagggtaag ccatacattt tcaaacgtga gggacaattt
     1681 atctgaatta aactcctgta tttgtttctg ataatcgtct gttgcaacct gaatggtttc
     1741 aaagtcgcag caatcatcca gggctttatt caaactgcaa ttcggattgt cagaagaatg
     1801 ggttccgcag caggagtgtc cttttgttcc cggctggcag cacgacgact cgtcgttact
     1861 agcaaacaac ccccaggaat acaactctcc ttcacagaaa tgcatggcta taaccggttg
     1921 aacagcaacc agcgccatga tattcagtaa aaatagagcc aacccttttt tcattttagt
     1981 tccttcaatt attatggtat gaacaacaac tgctcaagaa agttcacaaa aatacgtttt
     2041 attttggaat actgcaaatc cgggaagatc gaacagtttc cgattttgtc aatgaatgga
     2101 cctgtttaac gatgaattgc tgtaattttc cggagatttt aggtcacaat atttaaaatg
     2161 attaattttg caacacttat ttgtaaaaaa atgaaaaccc ggtcgctgaa aaaattcatg
     2221 tatctgtcca ttgctgcagc agtggtaact atcggtttga agtttacggc ttatttaaaa
     2281 accgggtcaa tgggtttttt ctccgatgcg ttggagtctt ttgtgaacct ttttgccgcg
     2341 gttatagccc ttattctcct gcatatttcc gaaaaacctg ccgatgaggg acacgaatac
     2401 gggcacggaa aagccgaata tttctcaagc gccattgagg gtgcgcttat cctgattgcg
     2461 gccttcagca ttatttacac gtcaatcccg cggattatcc atccgcaacc gctggaaaac
     2521 ctgggtatag gaacgttgtt ctctgttggg gcatccttgg tgaacctgtt tgcaggaact
     2581 ctattgattc aaaacggaaa gaaacaccgg tcaatggtgc tcgaggctga cggaaagcac
     2641 ctgatgacag atgtgtggac ttcagcaggg gttattgcgg gggttttggt ggtgaaattt
     2701 acagggcttc tggtactcga ccccatcatc gctattcttg ttgcgttgaa cattgtttac
     2761 acagggtata aactcatcag caggtcggca agcggactga tggatgcttc catcccgcag
     2821 gaagaaatca aacagattac gggttatttc gattcactga aaggaaagaa catcgattat
     2881 cattcgttgt tgacacgtca ggccggacac cggaaattca tatcgtttca tttgctggtt
     2941 ccgggagact ggacagtaaa agaaggccac gactacgccg aaataattga acagcgcatc
     3001 gaaaatatgt tcgatgatac ggttaccgtc accacccaca ttgagcccat tgaagatcca
     3061 ttgtcgctga acgacatcaa aatcgaccgg atgaattatt ccgacgatga cgattttgat
     3121 acctgatcaa ctgacttttg aaaaccgggt cagggaaaaa tccccgggca tcaatatgct
     3181 ctttaattta tcctccggct ccaccgccac gaaaaagtat gctgccaggt tatcgtcttt
     3241 cagcttcagg aaattatcct ctttttccca cgttccattg gaaagttcca acgaataaaa
     3301 ccgtatggaa taggttccgt catcatgcgc acgaaacgta aactccggat tgaaatcgga
     3361 ttgataaaaa ccatttctga atttattttt gtaagtattc ttatctatcc ttatcttttc
     3421 cctgtaactg agcagctcac tgcgcgacag gaaatcagtt gaaattgata cgggggacga
     3481 gggcttatcc gagcttttta caaaataatt taattgcatc catctgaatc cgtccttcac
     3541 cataaaaatc ttgtttcccg taacaggttc cagggtaatg acatattgat ttgtcccgtc
     3601 ggtcagcgtg tagttgccgt tatcttccac cgt
//
LOCUS       DAOSDR010000328         3631 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrimonas sp. isolate AMR_MDS_1826
            NODE_9005_length_3631_cov_4.322987, whole genome shotgun sequence.
ACCESSION   DAOSDR010000328 DAOSDR010000000
VERSION     DAOSDR010000328.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863355
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrimonas sp. (wastewater metagenome)
  ORGANISM  Petrimonas sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Dysgonomonadaceae; Petrimonas.
REFERENCE   1  (bases 1 to 3631)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3631)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:10:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,720
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 35
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3631
                     /organism="Petrimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9005_length_3631_cov_4.322987"
                     /isolate="AMR_MDS_1826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2023866"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            262..912
                     /gene="rpe"
                     /locus_tag="PLI30_11305"
     CDS             262..912
                     /gene="rpe"
                     /locus_tag="PLI30_11305"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005842952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HOI80176.1"
                     /translation="MNHLVAPSLLSANFLNLEKDIEMLNRSEADWIHLDIMDGVFVPN
                     ISFGFPVLDLLKERTSKSLDVHLMIVQPEKFVDEIAAAGTEYMNVHYEACTHLHRVVS
                     EIKKRGMKAGVTLNPHTPVLLLEDIIRDLDMVLLMSVNPGFGGQKFIEHSLEKVKVLK
                     ELILRKNARALIEVDGGVNLETGKRLVEAGADVLVAGNFVFSSQDPIHTIHQLKSL"
     gene            949..2910
                     /locus_tag="PLI30_11310"
     CDS             949..2910
                     /locus_tag="PLI30_11310"
                     /inference="COORDINATES: protein motif:HMM:NF015714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0030420 - establishment of competence for
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HOI80177.1"
                     /translation="MSDLVAKIPFIRLLLPAVASISISAFLIELTYSWVICMAGVGIM
                     AVSFFISERKQFTCRWLFGAGLFIFVFGLFSLLFQQKIEESQWRFADKPTVCIGTVVD
                     VPREKPRSFACNVKISYPVSHKIVAYLQKEDRVRNITPGDEIVFVAQIRTFKNFGNPD
                     DFDYVRFMRNNGFSGTTYLSSSNWHPTGKEHASLYVSAQKVRKKALEFYRMFELDNDA
                     YSFISALTLGYKHYLTNELLEAFRASGTAHVLAVSGLHVGIVYGVFAFLFSFLGKTGR
                     RSVIKQIMVIAALWLYAFVTGLSPSVLRATLMLTIASVGWMRGRSGFTLNTLAAAAFF
                     ILFLNPMSLFNVGFQMSFIAVLAILSFSPVLDSLYQPKNKIIKKSWGLFSVSISAQAG
                     IFPIALHYFGTFPTFFFIANMLIVPLIGVIIYACIPVILLTGLKPFQFVIVDWLYPVF
                     GWILKGLIFVVLKVVCFIETLPYAQLSDKPISTLQMMMLLFIVVTVFKFFTHKRVASL
                     IAGLTCSLFFILTFTYAELSRKPVQLAVFNKPGFSDIGLYVDEKRVYFDVKENGFIQH
                     PSTSILRLSGSSYSHVETSRPLEIDVLILSHDPAFSMMQLTNIFRTGQIVLDSSIPLY
                     GRIRLMRECEKLGISCHDVGEDGAYLINL"
     gene            2935..>3631
                     /locus_tag="PLI30_11315"
     CDS             2935..>3631
                     /locus_tag="PLI30_11315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019540209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HOI80178.1"
                     /translation="MNYFEPLSEIIDKSSVQQVQELIENSSQILITTHLSPDGDALGA
                     SLGLYHFLKLKKKDVKLMVPNSFPYFLKWMTGSGEILVYEYNPKAAQVIFDHADLIFS
                     LDYNIPKRVGNMASLLEKSSARKVLVDHHLFPGDNYDVVISYPGISSTSELIFRLLYQ
                     ADRYEEIDKTVAECIYCGMMTDTGGFTFNSNNPEIYLIISLLLRKGIDKDRIYSLVYN
                     NLTEDRFRLLGFTL"
BASE COUNT          930 a          710 c          821 g         1170 t
ORIGIN      
        1 atcttgaact tagtccctga taataaatga atgataaagc cgttttaaaa taaaagagac
       61 agaaatggct aatcctgatt ttatccgacg attctccttg tcccacgaat ccgatgcaaa
      121 agttccggac cgtttaagca tatgtattgt tcttcaatat gcaagatcgc tgcgtataga
      181 gaaaaaactg cctactcatt taggtttgtt catcatcaat tgattttttt attatctttg
      241 acactcaaaa tggataacga tatgaatcat ctggttgcgc cgtcattgct ctcggcgaat
      301 ttcctaaatc ttgaaaaaga tatcgaaatg ctgaatcgga gtgaagccga ttggatacac
      361 cttgatatca tggacggtgt ttttgttccc aatatttctt ttgggttccc ggttctggac
      421 ctgttgaaag aaaggacgag caagtctctg gatgtgcact tgatgattgt tcaacccgag
      481 aaattcgtag acgaaattgc cgctgcgggt acggaataca tgaatgtcca ttacgaggca
      541 tgtacacatt tgcatcgtgt ggttagcgag atcaaaaagc gcggaatgaa agccggggtt
      601 actcttaatc cgcacactcc ggttttgttg ctcgaagaca ttatccgaga tttagatatg
      661 gttttactca tgtcggttaa tcctggtttt ggcggtcaaa aattcatcga acattcgctg
      721 gagaaagtta aggtgttgaa agaattgatt cttcggaaaa atgcccgggc acttattgag
      781 gttgacggag gagtgaatct tgaaaccgga aaaaggctgg tagaggctgg agctgatgtg
      841 ttggttgccg gaaattttgt tttctcttct caagatccca tacatactat tcatcaactg
      901 aaatcgttgt aattctctga aaaacactcc ctcataagcg tgacagttat gagcgatctt
      961 gtggctaaaa taccctttat caggttgttg cttccggcag tagcctctat atcgatatcg
     1021 gcttttctga ttgaactgac ttattcctgg gtaatctgca tggcaggagt gggaataatg
     1081 gcagtctctt tttttatttc ggagagaaaa cagttcacct gtcgctggct ttttggagca
     1141 ggtttgttta tttttgtttt tggcttgttt tctcttttgt tccagcaaaa aatagaggaa
     1201 tcccaatggc gttttgcaga caaaccgacg gtctgtatag gtacagttgt tgatgttccg
     1261 cgggaaaaac cgcggtcatt tgcctgtaat gtgaaaatca gctatccggt aagccataaa
     1321 atcgtggctt acctgcaaaa ggaggataga gtgaggaaca ttacccccgg ggatgaaata
     1381 gttttcgttg cccagatccg gacttttaag aacttcggaa atcccgatga cttcgactac
     1441 gtccgattta tgcgaaacaa cggtttttca ggcaccacct atttgtcttc atctaattgg
     1501 catcccaccg gaaaagagca tgcctcgttg tatgtttcgg cgcagaaagt tcgaaaaaag
     1561 gcattggagt tttaccgtat gtttgagttg gataacgacg cttattcgtt tatttccgct
     1621 cttacgctgg gttacaagca ctatttgaca aacgaactgc tggaggcttt tcgtgcgtca
     1681 ggaacagctc acgtgttggc tgtaagtggg ttgcatgtgg gaattgttta tggtgttttt
     1741 gcatttctgt tttctttttt agggaaaaca ggcaggaggt ccgtgataaa acaaatcatg
     1801 gtcattgccg ctttgtggct ttacgctttt gttacggggc tttctccatc ggttctccgg
     1861 gcaaccctga tgctcacgat tgcttctgtg ggatggatga ggggtagaag tggctttacg
     1921 ctaaatacgt tggctgcagc tgcctttttt atcctttttt taaatccgat gagtcttttc
     1981 aacgttgggt ttcaaatgag cttcatagct gttctggcta tcttatcctt tagtccggta
     2041 ctggatagcc tgtatcagcc taagaataag ataataaaaa aaagctgggg tttattttct
     2101 gtttccatat ccgctcaagc aggtattttc cccattgctt tgcattattt cggtactttc
     2161 ccaacctttt tctttatcgc caatatgttg atagtgccgt taataggtgt gattatctat
     2221 gcttgtattc cggtgattct ccttacagga ttgaaaccct tccagtttgt aattgtcgac
     2281 tggctttatc cggtttttgg atggattttg aaagggttaa tcttcgttgt attgaaggtc
     2341 gtctgcttta tcgaaaccct tccatacgca caattgtcag ataagcctat ttctacacta
     2401 cagatgatga tgttgctctt tatcgttgtt actgttttta aatttttcac acataagaga
     2461 gttgctagcc tgattgctgg tttaacatgc tccctgttct ttatcttgac attcacgtac
     2521 gctgaactat cccggaagcc tgttcaatta gccgtattta ataaacccgg ctttagcgat
     2581 atcggtcttt acgtggatga aaaacgggtt tacttcgacg taaaagaaaa cggttttatc
     2641 caacatccat cgacgtcgat actgcgtttg tcagggagta gttattcgca tgtggaaaca
     2701 tcccggccgt tggaaatcga tgtgttgatc ctgtcgcatg acccggcctt ctcaatgatg
     2761 caactgacga atattttccg taccggacaa atcgtgctag acagttcaat tcctctgtac
     2821 ggcagaatac ggttaatgag ggagtgtgaa aagctgggga tttcctgtca cgatgtggga
     2881 gaagatgggg catatctcat aaatttgtag taattttgat cttttctata aaaaatgaac
     2941 tatttcgaac ccttatcgga gattattgac aagtcatccg tacaacaggt gcaagagctg
     3001 atcgaaaaca gcagccagat attgattacc acacacctct ctcccgatgg cgatgcattg
     3061 ggggcctccc tagggctgta tcattttctg aagttgaaaa agaaagatgt caagctgatg
     3121 gttcccaatt cgtttcctta ttttttgaaa tggatgaccg gatccggtga aattcttgtt
     3181 tacgaatata atccgaaagc agcacaggtt attttcgatc acgctgatct gattttcagt
     3241 ctcgattaca acatacccaa acgagtaggg aatatggcat ctcttttgga aaaatccagt
     3301 gcccgtaaag tgcttgttga tcatcatctt tttcccgggg ataattacga tgtggttatc
     3361 tcttatcctg gaatttcatc gactagtgag ctgattttcc gtttgctgta ccaggcagac
     3421 cggtacgaag aaatcgacaa aacggttgca gagtgtattt attgtgggat gatgaccgat
     3481 accggtggtt ttacgtttaa ctccaataat ccggaaattt atctgataat cagtttattg
     3541 cttaggaaag ggattgataa ggaccggatt tattcactgg tttataacaa ccttacggaa
     3601 gaccggttcc ggttgctggg cttcaccttg t
//
LOCUS       DAOSDR010000329         3613 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrimonas sp. isolate AMR_MDS_1826
            NODE_9068_length_3613_cov_3.625070, whole genome shotgun sequence.
ACCESSION   DAOSDR010000329 DAOSDR010000000
VERSION     DAOSDR010000329.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863355
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrimonas sp. (wastewater metagenome)
  ORGANISM  Petrimonas sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Dysgonomonadaceae; Petrimonas.
REFERENCE   1  (bases 1 to 3613)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3613)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:10:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,720
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 35
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3613
                     /organism="Petrimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9068_length_3613_cov_3.625070"
                     /isolate="AMR_MDS_1826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2023866"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1570)
                     /locus_tag="PLI30_11320"
     CDS             complement(<1..1570)
                     /locus_tag="PLI30_11320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI80179.1"
                     /translation="MKKIIIPLFVLINIILMSCVQKNESGNLENDVVIENEQFKLVIG
                     NNAAAKSLKLKSTGEECLMQGENMAIISVTQERPYHNEIKLGHPNKKTTYQADTIYRE
                     GDKLIVGFELIPYKAVIQVKETPDYIGFSLYDFITDGVYPSYLKITPPPATEVCILQL
                     PIRNRENFGEWLNVSWDNKAAINVLATDEYAYIDFEKRNGYKILKASAIKDIKFKGTG
                     AALIVSESDKLLDNIAQIENDFNLPKGVESRRNDFINASYYWTANINPTNVDQHIKYA
                     KMGGFRLMNIYYPSFEGGGGYGIIGSYEIDKTLYPNGKEDLQKMLDKIKAEGIIPGVH
                     FLHSHIGRKSQYVTPVPDYRLNLVKTFNLSENLGRDDTTVYVEQNPEGSVMAEGCRVL
                     KVGTELISYENFTTVPPYKFTGCKRAIDNTTVNSLPKGYSIGILDVSEFGATSVYINQ
                     KTSLQDEIAEKIAAIYDAGFQFFYFDGSEGVDPPFGINVALAQYRVFKRLKPQPLFAE
                     GAAKTHFSWHMLSGG"
     gene            complement(1594..>3613)
                     /locus_tag="PLI30_11325"
     CDS             complement(1594..>3613)
                     /locus_tag="PLI30_11325"
                     /inference="COORDINATES: protein motif:HMM:NF013300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004565 - beta-galactosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="beta-galactosidase trimerization
                     domain-containing protein"
                     /protein_id="HOI80180.1"
                     /translation="AFPFYFFVNGLREVRNMSKIYYMKHKPKYCLFYDNHTMKAIPDV
                     GENFNVEVFTDRIKACGVDYLVFHARCNQGLAYYNTRIGTRHPSLKYDLFGKLSEACQ
                     RKGIALGAYFNAGISSEEGLQHREWTTLYFDGRSLREPRFTPHVRTMCYNSGYRDHLI
                     EMVKEVLYQYPVGGLMIDCLINYPCVCPVCVQEMKKRNMDWSDLSAVTRFAEISAVRL
                     AKDIAEEAMKINPDLLISNNYPSYEELLGINTYFDVVSLPASDSGYEFMPVMAHYLRT
                     LGDYPVLNMTGRFYDWGDFGGLRPGEAIKSELLYGLANGMRPNIGGHFHPRGDLENAV
                     LDRVERIYRELQTMEPWFDNAKNITEIAIVYPKKIANIRGDRQLKSAVRMLSELKQQF
                     DVVTLASDWSKYKVLIFPDDILFDEETARRVKKHVNEGKAVISTGFSGLDPEKRHFVL
                     EQEWGIKFLGENPFDPAYFTVGENCNEGLPDMPLSLYSNGIEVEASGKTRTEARLVKP
                     YHNRDWDGEYAFYYNPPDRVTEKPALTINGQVAHFSHRIFSGYYDKASVELRTVFSNV
                     LDRFLAKPLVKTKNLPSFSRVFVTEQPKRRMVHLLSYLPEMRGNTQMIEEPIELDNIT
                     LSLRHDDRNFKNIYLAPEKVRLSYTLEGDYVHINVPKSKGYSLLVIEE"
BASE COUNT          970 a          860 c          681 g         1102 t
ORIGIN      
        1 ttcctccact caacatatgc cagctaaaat gtgttttagc agccccttct gcaaataaag
       61 gctggggttt taaccgttta aataccctgt actgcgctaa agcaacgttg ataccgaatg
      121 gaggatccac cccctctgaa ccgtcaaaat agaaaaattg aaatccggca tcataaattg
      181 ccgcaatttt ttcagcaatt tcatcttgta agctggtttt ctggtttata taaacactgg
      241 tagcgccgaa ttcactgaca tccagtatac caatggagta tcccttggga aggctgttca
      301 ccgtagtgtt atcgatcgcc cttttgcatc ccgtaaattt ataaggtggt acagtagtaa
      361 aattctcata tgaaataagt tccgttccta ccttcaaaac ccggcatccc tccgccatga
      421 cggatccttc gggattttgt tctacataca cagtcgtatc gtcccttccc aggttttccg
      481 acagattaaa tgtttttacg agatttagcc ggtaatcggg aacgggagtt acgtactggc
      541 tctttctgcc gatatgtgaa tgaagaaaat gaacacccgg aatgatacct tccgctttaa
      601 tcttatccaa cattttctga agatcttctt ttccattggg atataacgtc ttgtcaattt
      661 cataacttcc aatgataccg tatcctcctc ctccttcaaa actggggtaa tagatattca
      721 tcaacctgaa accacccatc ttggcgtatt tgatgtgttg atcgacattc gttggattta
      781 tgtttgccgt ccaatagtat gaagcattga taaaatcatt tctacggctt tctacgcctt
      841 tgggcagatt aaagtcattc tctatctgag caatattatc cagcaattta tcggattcag
      901 aaacaattaa tgcagccccg gtgcctttaa attttatatc cttaatggca cttgccttca
      961 atattttata cccgtttcgt ttctcaaaat cgatataggc gtattcatct gtggctaaca
     1021 cattaatagc agccttatta tcccaactga catttagcca ttctccaaaa ttctcccggt
     1081 tacggatggg taattgtaaa atacatactt cagtagcggg aggaggagtt attttaaggt
     1141 acgagggata aacgccgtcc gtaataaaat cgtaaaggct gaaacctata taatcaggtg
     1201 tttccttgac ttgaataacc gctttgtacg gaattaattc aaatccaaca atgagtttgt
     1261 ctccctcccg ataaatagta tcggcctggt atgtcgtttt cttattgggg tgacccaatt
     1321 ttatttcatt gtggtacggc ctttcctgag tcactgagat aattgccata ttttctccct
     1381 gcatcaaaca ctcctcgccg gtagacttca gtttcaaact cttagcagcg gcattattac
     1441 cgataactaa tttaaattgt tcattttcga ttacaacgtc attttcgagg ttgccacttt
     1501 catttttttg cacacatgac attaaaatga tgttaatcaa tacaaataat ggaataataa
     1561 tttttttcat aactgcatat ggttgttatt tgtttattct tcgataacca gcagagagta
     1621 tcctttgctt ttaggtacgt tgatatggac ataatcacct tcaagggtat aagacagcct
     1681 caccttttct ggtgccaggt agatgttctt gaaatttcta tcatcgtggc gaagggatag
     1741 ggttatgttg tcaagttcaa tgggttcttc gatcatttgc gtatttcccc tcatctcggg
     1801 cagataagaa agcaaatgca ccattcttct tttgggttgt tccgtgacaa aaacccggga
     1861 gaatgacggt aaattttttg tctttactaa cggtttggca agaaaccggt cgagcacgtt
     1921 ggaaaagact gttcttaact ccacggatgc cttatcgtaa tacccggaga atatacggtg
     1981 gctgaaatgg gccacctgcc cgttaattgt aagcgccggc ttctccgtca ccctgtctgg
     2041 cggattataa tagaaagcat attccccatc ccaatcccgg ttatgatatg gttttacaag
     2101 acgtgcctcc gttcttgttt taccggacgc ttccacttca atgccgttgg agtataaaga
     2161 gagcggcatg tccggcaacc cttcattaca attttcccct acggtaaaat aagccggatc
     2221 aaacggattt tctcctaaaa atttgatgcc ccattcctgt tcgagcacaa aatgtctctt
     2281 ctccggatca aggccggaga accccgttga aatgaccgcc tttccttcat tgacgtgttt
     2341 cttcacgcgt cttgccgttt cctcgtcaaa gagaatgtcg tcgggaaaga tcagcacctt
     2401 gtacttgctc cagtccgatg ccagcgtcac cacatcgaac tgctgcttca attcgctcaa
     2461 catgcgcacc gcgcttttga gttgtctgtc gccccgtatg tttgcaattt tctttggata
     2521 gacgatggct atttcagtta tgttcttggc gttgtcgaac cagggttcca tggtctggag
     2581 ttccctgtat attctttcca ccctgtcaag gacggcattc tcgagatccc ccctggggtg
     2641 aaaatggcct ccgatattgg gacgcatccc gttggccaac ccgtaaagca actccgactt
     2701 gatggcttcc ccgggacgta atcctccaaa atccccccaa tcgtaaaacc ggcccgtcat
     2761 attcaaaacc ggatagtcgc ccagcgtcct taaatagtgg gccatcacag gcataaactc
     2821 gtatccgcta tccgatgccg gtaaagacac cacatcgaaa taggtattta ttcccaataa
     2881 ctcttcgtaa ctcggataat tattggaaat aagtaaatcg gggtttattt tcatcgcttc
     2941 ctccgcaata tcttttgcta aacgtactgc tgaaatttcc gcaaaacgag tgaccgcgct
     3001 taaatccgac caatccatat ttcttttctt catctcttgt acacagacag gacatacaca
     3061 gggataattg attaagcagt caatcatcag ccccccaacg ggatattggt aaagtacctc
     3121 tttaaccatt tcaatcagat gatccctata tcccgagttg taacacatgg tacggacatg
     3181 cggcgtaaaa cgtggctcac gcaacgacct gccatcgaaa tacaaggttg tccactcccg
     3241 atgctgcaat ccttcctcac tactaatacc cgcattaaag tatgctccca atgcaatccc
     3301 ttttcgttga caagcttctg acaatttgcc gaaaagatcg tattttaagg aagggtgtct
     3361 tgtcccaatt cgggtattat aatatgcaag cccttgatta caacgagcat gaaacacgag
     3421 ataatcaact ccacaagctt tgatcctgtc agtaaacacc tctacattga agttttctcc
     3481 cacatcggga atagccttca tcgtatgatt atcatagaat aaacagtatt taggtttatg
     3541 tttcatgtaa taaatcttac tcatgtttcg cacttccctc aaaccattga caaagaaata
     3601 gaagggaaat gca
//
LOCUS       DAOSDR010000330         3607 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrimonas sp. isolate AMR_MDS_1826
            NODE_9088_length_3607_cov_3.435811, whole genome shotgun sequence.
ACCESSION   DAOSDR010000330 DAOSDR010000000
VERSION     DAOSDR010000330.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863355
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrimonas sp. (wastewater metagenome)
  ORGANISM  Petrimonas sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Dysgonomonadaceae; Petrimonas.
REFERENCE   1  (bases 1 to 3607)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3607)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:10:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,720
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 35
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3607
                     /organism="Petrimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9088_length_3607_cov_3.435811"
                     /isolate="AMR_MDS_1826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2023866"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(219..291)
                     /locus_tag="PLI30_11330"
     tRNA            complement(219..291)
                     /locus_tag="PLI30_11330"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(256..258),aa:Thr,seq:cgt)
     gene            complement(353..1903)
                     /locus_tag="PLI30_11335"
     CDS             complement(353..1903)
                     /locus_tag="PLI30_11335"
                     /inference="COORDINATES: protein motif:HMM:NF024753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HOI80181.1"
                     /translation="MKKTLFPFLFLCFTVYINAQRTANGLFVTKDTVIGKAFDGNDIV
                     AKCYQPQHKIYRWHWDGFSDNLLLELRQANKKGTSFKNEGNVVMIDLEDKSVKWSRNV
                     NYNNSETKQQGRYLFLAEKKKNLCLDPETGKVLWENKNDFYFIDPELNIGVGYPLQSM
                     SNNLSAIDLSNGNILWTKPVDRTRGWDDAYMLTDSILLVSVNGIQALELPTGKSWSYK
                     ATTSQKKIGKMIATNAAGILLGVLTGTVMYQTNPDEVTEMVSNMLIDEENTIVLASRD
                     MISRVGNSGEIQWSIPLPEKITSKSSLFLKDSVIYMINRGYAFYNGGFSTVGDPYFAA
                     FNLNDGTQRYLNMIPEKKEFIRNFQVINDVLFLVFENKIVTYSLADGSLLTEKIIGLE
                     KGERLDTFVESGIYALQGDSTFMDLAAAYPEQNLIMTSENRVISLTDSLETLITYDKK
                     DVFKKTIDNGNYKFLTNDEKEFTVCDYSGKALMKFRASPNMFMRNNKLYAFDKDLFWE
                     LSLSAFVR"
     gene            complement(2015..>3607)
                     /gene="secF"
                     /locus_tag="PLI30_11340"
     CDS             complement(2015..>3607)
                     /gene="secF"
                     /locus_tag="PLI30_11340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019540408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HOI80182.1"
                     /translation="GRFSAEEAGDLENVLKSGKMKAGVRIVQEDVVGPSLGQEAIRDG
                     FVSFVIALIVLFIFTCLLYGIVPGMVINGALLINFFFTLGVLAAFQAVLTLPGIAGMI
                     LSLAMAVDANVLINERIKEELANGKSLRKALEDGYKNAFSAILDSNLTTIITGIILAI
                     FGTGPIKGFAVTLIIGISVSFLTAVFLTRLVYEYQFEKNRWQKLTFNTGFSRAIFNVY
                     NFDFIKNSKKIILAILIFGVVSIGSLFTLKLNTGIDFTGGRNYIVRFDQPVKTGDIEQ
                     ALQPVLGGSAQVITIGSSNQVRISTNYMIDSESATVEDDLVTLLGEGLKDYIAPGSSI
                     NDHILSSQKVGPSVAEDTMKNAFLALTIALICMGIYIFARFRNWAFSVGTVAALSIDT
                     FAVIGFYSLLWKVMPFSMEIDQTFVAAILTIVGYSINDKVVVFDRFREVHQLYPKREL
                     RALFNDSMNAVLARTINTGLSTILVILCILFLGGDAVRSFVFAMLIGVVVGTVTSLFI
                     ASPVAYSIMRTQQEKKQLEPKK"
BASE COUNT          990 a          809 c          801 g         1007 t
ORIGIN      
        1 ggagcaggag tttaatgctt atgatgccag cctttccgga gcgggaataa gcagtacgaa
       61 atttaaactg aacaaactgt tgaactttat accgcattac cggcccgatg aaaacttccg
      121 ggattgggtg catcaggcgg aagaacggtt tatccgggag atctgcggca ccaactaaaa
      181 aagcccttag ccgggcggct aaaggctttt ggtgaaagag ccacaagcga gattcgaact
      241 cgcgaccgac gcgttacgaa tgcgttactc tacctactga gctattgtgg caacgtttcg
      301 ggttgcaaaa ttataaaaaa taaccgaaac cgactaaaat tacatcaagt ttttatcgta
      361 cgaacgcact taggcttaat tcccaaaaca aatctttatc gaaagcatac aatttgttgt
      421 ttctcataaa catattgggt gatgcccgaa atttcattaa cgcttttccc gaataatcac
      481 aaacggtgaa ctctttttca tcgttagtca ggaatttata attgccgtta tcgattgttt
      541 ttttgaaaac atccttcttg tcatacgtta ttagtgtttc taaactgtca gtcagcgaga
      601 tgactcgatt ctcagacgtc attatcaggt tctgttcggg gtaagcggca gccaaatcca
      661 taaaagtaga atcgccctgc agggcataga ttccggattc cacaaaagta tccagtcgtt
      721 cacctttctc gagaccgatt attttttccg tcagcaggct gccgtcggca agggaatagg
      781 tgacgatttt gttttcgaaa accaggaaaa gcacgtcgtt gataacctga aaattccgga
      841 tgaactcttt tttctcggga atcatgttta aataccgttg ggtgccgtca ttaaggttaa
      901 aagcagcgaa ataagggtcg ccaaccgtgg aaaaaccacc gttgtagaag gcataaccgc
      961 gattgatcat ataaatgacc gaatctttca ggaaaaggga cgatttcgat gtgatttttt
     1021 caggtagcgg gatactccac tgaatttctc ccgagtttcc tacccgggaa atcatatctc
     1081 gggaagcgag aacgatcgtg ttttcttcat cgatcagcat atttgaaacc atttccgtaa
     1141 cctcgtcggg gtttgtttga tacatgacgg ttcccgtgag aacccccagc aaaatacctg
     1201 cggcgttggt tgcgatcatt ttcccgatct tcttttgact ggtggttgct ttgtacgacc
     1261 agctctttcc tgtaggcagt tcaagtgcct gaattccgtt gacggacacc agaagaatgg
     1321 agtcggtcag catgtaagca tcgtcccagc cgcgtgtccg gtcaaccggc ttggtccaca
     1381 gaatatttcc gttggataaa tcgatggccg ataagttatt cgacatgctt tggagcggat
     1441 aacccacgcc tatgttcagc tccgggtcta taaaataaaa gtcatttttg ttctcccaaa
     1501 gtacctttcc tgtttcggga tcgaggcaaa ggtttttctt tttttcggcg agaaagaggt
     1561 acctcccctg ttgttttgtt tcggagttgt tgtaattgac attccggctc cattttacag
     1621 atttatcttc cagatcgatc atcacgacgt ttccttcatt cttgaacgag gttcctttct
     1681 tgtttgcctg tctcagttcg agtaataggt tgtctgaaaa accatcccaa tgccaccggt
     1741 aaattttgtg ttgtggttgg taacatttgg ccacaatatc gtttccgtca aacgcttttc
     1801 ctataacggt atcttttgta acaaaaagcc cgttggcggt acgttgcgca tttatgtata
     1861 cggtaaagca taaaaaaaga aagggaaata gcgtcttttt cataatcttg tgatttatta
     1921 cgcggtaaaa ttagtacttt gttctcaaat gcaaaaaaaa acgggttgcc tggcaacccg
     1981 ttttaacata ataataccta attgaatata ttgtttattt tttaggttcc aattgtttct
     2041 tttcctgctg cgttctcatg atcgagtaag ctacaggtga agcaatgaac aaacttgtta
     2101 cagttcctac aactaccccg atcaacatgg cgaacacgaa acttcttacg gcatcacctc
     2161 ccaggaacag aatacacaag ataaccagga tcgtactcaa accggtgttg atggtacgtg
     2221 ccaatacggc gttcatagag tcgttgaaca gtgcgcgcag ttcgcgtttg ggatacaact
     2281 ggtgcacttc acggaaacgg tcgaacacaa ccactttgtc gttgatggag taacccacaa
     2341 tggtcaggat agccgcaaca aatgtctggt cgatttccat tgagaacggc attaccttcc
     2401 aaagaagtga ataaaaaccg ataacggcaa acgtgtcgat agatagtgct gcaactgttc
     2461 ctacggagaa tgcccaatta cggaaacggg caaaaatgta gatacccata cagattaaag
     2521 cgatggtaag cgccaggaag gcattcttca tcgtgtcttc ggcgacactt gggcccactt
     2581 tctgtgaact caggatgtgg tcgttgatgc tgcttccggg tgcaatgtaa tcttttaacc
     2641 cttccccaag cagtgtaact aaatcgtctt ctacggttgc gctttcggaa tcaatcatat
     2701 agttggtaga aatacgcacc tgattgcttg agccaatggt gatcacctgc gccgaaccgc
     2761 ctaagaccgg ttgcaaagct tgttcaatat ctcctgtttt taccggctgg tcgaaacgca
     2821 caatgtagtt gcgtccaccg gtaaagtcga tacccgtatt caatttcaag gtaaagagtg
     2881 aaccgatgct gacaactcca aaaatgagta ttgccagtat gattttcttg ctgtttttga
     2941 tgaaatcgaa gttatatacg ttaaagattg ctcttgagaa tccggtattg aaagtgagtt
     3001 tttgccacct gtttttttca aattgatact cgtaaactaa acgagttaag aaaacggcgg
     3061 ttaggaacga aacggaaata ccgatgatca gtgtaacggc aaatcctttg ataggacctg
     3121 ttccgaagat cgccaggatg ataccggtaa taatggttgt caggttagag tcgagaattg
     3181 cagagaaagc attcttataa ccgtcttcca gtgctttacg caacgatttt ccattggcca
     3241 gttcttcctt gatacgttcg ttaatcagca cgttggcatc cacagccata gccagagaca
     3301 agatcatccc cgcgatgccc ggcagtgtga ggacagcctg gaatgccgcc agaacaccca
     3361 gcgtgaagaa gaagttgatg aggagtgcac cgttgattac cattcccgga acaattccgt
     3421 acagtaaaca ggtgaagata aacaatacga taagggcaat gacgaacgat acaaaaccgt
     3481 cgcggatggc ttcctgcccc agtgagggtc ccacaacgtc ttcctgaacg atgcgtacac
     3541 ccgctttcat tttgcccgac ttcagcacgt tctccaagtc tcccgcctct tctgcggaga
     3601 aacgccc
//
LOCUS       DAOSDR010000331         3579 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrimonas sp. isolate AMR_MDS_1826
            NODE_9170_length_3579_cov_3.623156, whole genome shotgun sequence.
ACCESSION   DAOSDR010000331 DAOSDR010000000
VERSION     DAOSDR010000331.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863355
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrimonas sp. (wastewater metagenome)
  ORGANISM  Petrimonas sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Dysgonomonadaceae; Petrimonas.
REFERENCE   1  (bases 1 to 3579)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3579)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:10:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,720
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 35
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3579
                     /organism="Petrimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9170_length_3579_cov_3.623156"
                     /isolate="AMR_MDS_1826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2023866"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..490)
                     /locus_tag="PLI30_11345"
     CDS             complement(<1..490)
                     /locus_tag="PLI30_11345"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HOI80183.1"
                     /translation="MIVTMNIKPRQLEIIEATGKLLTTSGLNGLTIKNLALEMNFSES
                     AIYRHFSSKEEIIITLLKYLNETVNKIFKESSASGDFEKDFTALFRKLTTYFMENPFY
                     VVAVFSEGLMNESARIGERILQLMNNLTKHVRTIIGEGQKKGLITRSLTSEQIVEIII
                     PAF"
     gene            complement(551..1195)
                     /locus_tag="PLI30_11350"
     CDS             complement(551..1195)
                     /locus_tag="PLI30_11350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI80184.1"
                     /translation="MKITILQSTLLAWMVSLGANLSAQTDEWKTALSKNGKITAKYNF
                     SSRINEDDDKVPVVKTITTTTESIGIDNCIALMKNIAKHKDFRGEKSSKLINKISENE
                     WSIYYYNDGMLLTPAVDGSYRMIFEEDRKNKTATFTLTADPTLIEKTDMARLTYAKEI
                     YSFKEVEGNQLEITIKTTVSPGFKVPFLMMKKAFPRKSFEHMEKFLEAAKKEKP"
     gene            complement(1256..1576)
                     /locus_tag="PLI30_11355"
     CDS             complement(1256..1576)
                     /locus_tag="PLI30_11355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI80185.1"
                     /translation="MIERKAFFISGERLTSIEHKKKILVIFDMSRDEVVGVETVDIED
                     RFVSTEELLLFLQKKYIRVIYVSEIDSKTENLFLDSGLVVKTSMTIKNDKLFNSLYLS
                     PPIF"
     gene            complement(1834..>3579)
                     /locus_tag="PLI30_11360"
     CDS             complement(1834..>3579)
                     /locus_tag="PLI30_11360"
                     /inference="COORDINATES: protein motif:HMM:NF039873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HOI80186.1"
                     /translation="DFDDLPIPFGCVAADVRSGREVVLREGSLPEAIRASMAIPGMFT
                     PVEKDSMLLIDGGVINNFPVDLVRSMGADRVIGVIFPPDEEAMEKSRGSITEVTEQLW
                     NFVGQEKRRRNIADTDLLITPEVHSFGMMDFQRPAIDTIISRGKEAAAAKRDELIALK
                     QSLGTEEAAPAVTSDKNPYIGLDTLVINSIRIEGVSPRETSQIARWIDIDKNSVTREE
                     LDEMTARIYSSGWFSRVYYRLEGEHPFDLVFTVEVKESNTLNLGIHFDSNDMAAILAN
                     TTIRLNTSLNSLFDITARLSRDPYLMIDYSINKGIFYKGGISYKISSNDLSIYERGSL
                     SYNLGVTRNALNLVFSEFYFGNIMLQLKAAMEHFHFYKALRSEFDPRHTEPNDQLYIN
                     YLINGVYDNLNRIDFPTSGQYFSFQYSLHTDNFVQLKNEIPLSVLKMNFYKPLPLTQK
                     ICITPRINARYVMNDSVPHMYRNLAGGRFDGHYLPQQISVQGSAGMEFLNNMVLLTDV
                     TLYYNFTPKNYLYANINFTADSHHLHHLLEGKSYLGANLGYSYLSVAGPLRVELGYSS
                     LSRRFHPYLSFGHYF"
BASE COUNT          923 a          796 c          761 g         1099 t
ORIGIN      
        1 tgaaggcagg aatgataatc tccacgatct gctccgacgt cagcgaacgg gttataaggc
       61 cttttttctg gccttcgcct ataatggttc gtacatgttt cgttaaatta ttcatcagct
      121 gaagaattct ctctccaatc cgggcactct cattcatcag cccttcagaa aagacagcaa
      181 ctacatagaa tgggttctcc ataaaataag tggtcagttt tctgaaaagt gccgtaaaat
      241 ctttttcaaa atctcccgat gcggaagatt ctttaaaaat cttgttcaca gtttcattca
      301 agtatttcaa caaagtgatg atgatttctt ccttacttga aaaatgcctg taaatagcac
      361 tttcggagaa attcatctcc aaagccaggt tcttgatcgt caggccgttt aaacccgaag
      421 ttgtgaggag tttcccagtt gcctcgataa tttccagttg cctcggttta atgttcattg
      481 ttactatcat tgttcatcct tttttagtag taaagaattg ttttattcct ggttggcaga
      541 gaatcagcgt tcagggcttc tccttttttg cagcctcgag aaatttttcc atatgctcaa
      601 aagattttct gggaaatgct ttcttcatca ttagaaacgg cactttaaat cccggcgaaa
      661 ccgtagtttt gatggttatt tccaattgat tcccttctac ctccttaaat gaatagatct
      721 cttttgcata ggtcaaacgg gccatgtccg tcttttcaat caatgtaggg tctgccgtaa
      781 gagtaaacgt ggccgtttta tttttcctgt cttcttcaaa tatcattcgg taggaaccat
      841 ccactgccgg ggtaagcagc attccatcgt tgtaataata gatggaccat tcattttctg
      901 agattttatt gatgagttta ctcgattttt ctccgcggaa atctttatgc tttgcaatat
      961 ttttcattaa tgcgatacag ttgtcaatgc cgatactttc tgttgttgta gtaatagttt
     1021 ttactacagg gactttatcg tcgtcttcat ttatgcgcga cgaaaaattg tatttcgccg
     1081 ttattttgcc atttttactg agtgcggttt tccactcgtc ggtttgggca gatagatttg
     1141 cgcccaaaga gaccatccat gccagaagcg tactttgtaa gatcgtaatt ttcattgaga
     1201 tttttgattt tttttagtaa ttgaataagc tgatttttat actgtttatc catttttaga
     1261 aaatcggagg agacaaatag agcgaattaa atagtttgtc gttttttatc gtcatggacg
     1321 ttttcacgac caggccggag tctaaaaaca aattttccgt tttactgtct atttccgaaa
     1381 cataaattac acgaatatat tttttttgaa gaaagagcaa caactcttcg gttgaaacga
     1441 atctatcctc tatatcaacg gtttctacac caacaacctc atcccgactc atatcaaaaa
     1501 tgaccaatat cttttttttg tgctcaatac tggtgagtct ctccccggaa ataaagaatg
     1561 ctttcctttc aatcatatcc cgattatttt tctccatttt caacattcaa acataaactg
     1621 atgaacaaat aatttatgcc gagaagagta agtgaataga catcaactaa aattccaaac
     1681 ataaaatgac aatcgattaa ttaatctcat aataagtaag tgagcaatca ctcacaaaga
     1741 tacaatcgaa attaaaaaat gcaacaggtt ttgaattatt taacgcaact ttccggttta
     1801 ttaacacaat gtgcgggcct ttcaactacg cggtcagaag taatgtccga aactaagata
     1861 agggtgaaac ctcctggaga ggctggaata ccccagttca acccgcaatg gtccggctac
     1921 cgaaaggtag gaatagccta agtttgcccc caggtaagat tttccttcaa gcaggtgatg
     1981 caggtgatgg ctgtctgcgg tgaaattgat gttggcgtag aggtaatttt tcggggtgaa
     2041 attataatac aatgtcacat ccgtcaacag caccatgttg tttaaaaact ccatgccggc
     2101 agagccctgc acggatatct gctgaggcag gtaatgtccg tcgaacctgc ctcccgctaa
     2161 gttgcggtac atgtgcggta cactgtcgtt cataacatac ctggcgttga tgcggggagt
     2221 gatgcagatt ttttgggtta acggtaacgg tttgtagaaa ttcattttca ggacgcttaa
     2281 tgggatttcg tttttcagtt gaacgaaatt gtcggtatgc agtgagtact gaaaagagaa
     2341 atattgcccc gatgtgggga agtcgatgcg gttgaggttg tcgtacactc cgtttatcag
     2401 gtagttgata tacagctgat cgtttggctc tgtatggcgg gggtcgaact cgcttcgcaa
     2461 cgctttataa aagtgaaaat gttccattgc tgcctttagc tgcagcatga tgttgccaaa
     2521 atagaattcg gaaaagacca ggttcagcgc gttccgtgta acgcccaggt tgtacgataa
     2581 gctacccctt tcgtaaatgc tcagatcatt gctgctgatc ttgtaactga tccccccctt
     2641 gtagaaaatc cctttgttga tggagtaatc gatcatcagg tagggatcgc ggctcaacct
     2701 ggcagtgata tcaaacagtg aattgagtga agtgttcaac cggatggttg tgttggcaag
     2761 tattgctgcc atatcgttgc tgtcgaaatg tatgcccagg tttagggtgt ttgactcttt
     2821 cacttcaaca gtgaaaacaa ggtcaaacgg atgttccccc tcgagccggt agtaaaccct
     2881 ggagaaccat ccgctactgt atatgcgtgc cgtcatctca tccagctcct cccttgtaac
     2941 gctgttcttg tcgatgtcga tccaccgggc aatctgactt gtctcccgtg gtgagacacc
     3001 ttcgatccgt atggaattaa tgaccagcgt gtcgagtccg atgtaggggt ttttatccga
     3061 tgtaacggcc ggagcagcct cttctgtgcc caacgattgt ttcaaggcaa tcagctcgtc
     3121 ccgcttagcg gctgctgcct ccttgccccg ggagataatg gtatcgatgg cgggacgttg
     3181 aaaatccatc attccgaacg agtgcacctc gggagtgatc agcaggtcgg tgtccgctat
     3241 gtttctcctg cgtttttcct ggccgacaaa gttccagagc tgctccgtca cttcggtaat
     3301 gcttccccgg ctcttttcca tggcctcttc gtcgggagga aagataactc cgatcaccct
     3361 gtctgccccc atgctgcgta ccagatccac cgggaaatta ttgatcaccc ctccgtcaat
     3421 cagcaacatg ctgtctttct caaccggtgt gaacatgccg ggtattgcca tgcttgcccg
     3481 tatcgcttct ggcaagcttc cctccctcag gacgacttcc ctgccgctgc ggacatcagc
     3541 tgccacacac ccgaaaggga tgggaagatc atcaaaatc
//
LOCUS       DAOSDR010000332         3573 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrimonas sp. isolate AMR_MDS_1826
            NODE_9190_length_3573_cov_3.599488, whole genome shotgun sequence.
ACCESSION   DAOSDR010000332 DAOSDR010000000
VERSION     DAOSDR010000332.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863355
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrimonas sp. (wastewater metagenome)
  ORGANISM  Petrimonas sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Dysgonomonadaceae; Petrimonas.
REFERENCE   1  (bases 1 to 3573)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3573)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:10:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,720
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 35
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3573
                     /organism="Petrimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9190_length_3573_cov_3.599488"
                     /isolate="AMR_MDS_1826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2023866"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..948
                     /locus_tag="PLI30_11365"
     CDS             <1..948
                     /locus_tag="PLI30_11365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019246119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HOI80187.1"
                     /translation="DTPVVMISGHGNIDTAVESIKKGAFDFIEKPLDLNRLLITIRNA
                     MDKSHLVTETKILKKKVNKKFEIIGESAAIHKVIEMADRVAPTDARVLITGSNGTGKE
                     LVARRIHDMSHRAAGPFVEVNCAAIPSELIESELFGHEKGAFTSAVKQRKGKFEQATG
                     GTLFLDEIGDMSLSAQAKVLRALQENIISRVGSDGAIKVDVRVVAATNKNLSEEIEKG
                     NFREDLYHRLSVILIRVPTLNERIEDVPLLAAHFISQICGEYGMPEKTILPEAIAELQ
                     NIQWTGNIREFRNVIERLIILCDKKITAQDVLNFAAPLK"
     gene            1420..2319
                     /locus_tag="PLI30_11370"
     CDS             1420..2319
                     /locus_tag="PLI30_11370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI80188.1"
                     /translation="MAQSGKFAKHCTLFLLFFTRQPATLGIPKVLLNAKSSQLAITTT
                     KKLKNMKSETLNFIKGIIIFFFIAVPLISFAQEKVTWDYPVKPGSAEWKKLSSHQEMV
                     QICQIPAGTLSFISTKELAQICLNYPLFFTMKAFNNLQLGFNQVSTEFNGFQELFKRD
                     DAGNVLLELYKAIKPQVILFKTTSLEKGKHIQNIFFIEFVLSQKEIIEKFTKAEIKQL
                     LEESLVKLEEKKQYGFSTFSTQTTSLVMCRVLENIKFDKYIQINTVFEKYKYFSNAII
                     LSDKEMIEDLKKITIDYLNDNSN"
     gene            2457..3092
                     /locus_tag="PLI30_11375"
     CDS             2457..3092
                     /locus_tag="PLI30_11375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI80189.1"
                     /translation="MEEWKELNSHQAMVEVCQIPKEILNTMCTIGLVDTYLDYPILFT
                     IFAFNNINEGLSQAVSEFNGFGQLLQKKDCATKFLSRYEPINPANIDPSWTSIEKGRF
                     MQTLRIMEVTLAFDPMGRNLTKEERKKLIEIGMEKLKIKEMHDYSGPSQITNLYLIGN
                     ILKLDGYQPFLDFLQRRNDIKAFLKGDLNSLYYVADGDTIKKYVESFLKQH"
     gene            3103..>3573
                     /locus_tag="PLI30_11380"
     CDS             3103..>3573
                     /locus_tag="PLI30_11380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI80190.1"
                     /translation="MNKHILVLIVLLSTCLMVKGQYDYEYETTIVQTPTGVNVDALSF
                     RYWIYNDFDANDIAIENFNWTNGYNCEIIENSTKYYNCHGYVWYNVEGNMSQVNLRWI
                     NNIDYYGNPTYNVQKYYSSSYSGGNPSYRQVSTNNRAYLKVSYFPRDHSALTTSN"
BASE COUNT         1148 a          738 c          731 g          956 t
ORIGIN      
        1 gacacgccgg tggtgatgat ctcgggccac gggaatatcg ataccgccgt ggaatcgatc
       61 aaaaaagggg cgtttgattt tatcgagaag ccgctcgacc tgaaccgcct gctgattacc
      121 atccgcaatg cgatggacaa atcacacctg gttaccgaaa ccaaaatcct gaaaaagaaa
      181 gtcaacaaga agtttgagat cattggcgaa tcggccgcaa tccacaaggt tattgagatg
      241 gccgaccgcg tggcgcccac cgacgcgcgg gtgctgatta ccggctccaa cggaaccggc
      301 aaagagctgg tggcccgtcg catccacgac atgagccacc gggctgccgg cccttttgtt
      361 gaggttaact gcgccgccat cccgtcggaa ctgatcgaaa gcgaactttt tggccacgaa
      421 aaaggggcgt ttacatcggc ggtgaaacag cgcaaaggga aatttgaaca ggctaccggc
      481 gggacccttt tcctggatga gattggcgac atgagcctgt cggcgcaggc aaaagtgctg
      541 cgcgcccttc aggaaaacat catcagccgt gtggggagcg atggcgccat taaggtggac
      601 gtgcgcgtgg tggccgccac caacaaaaac ctctcggaag agatcgaaaa gggcaacttc
      661 cgcgaagacc tctaccaccg cctcagcgtt atccttatcc gggtgccaac cctgaacgaa
      721 cggatcgagg acgttccgtt gctggccgcc catttcatca gccagatttg cggggaatac
      781 ggaatgcccg aaaaaacaat cctccccgag gccattgccg agttgcagaa tattcagtgg
      841 accggcaata tccgcgaatt ccgtaacgtc attgagcggc tgatcatcct gtgcgacaaa
      901 aagattaccg cccaggatgt gctcaatttt gccgccccgt tgaaataggc cactcccggc
      961 ctccgtacca gacgggctgt ctgaaaagtc aggtttttat ttacagaact tgcaaattgt
     1021 aatcgcgcct ttgttttacc cctcccaact tcccctgaag gggcggctgg gaagtctcct
     1081 gcagggaatt gaggggtttt aaagatattg ttacttacca agtctaagcg attgaaaacc
     1141 taaaatctct tttcagacag cctcgttttt tttaccagcc ccacgcaacc tttccccagc
     1201 ttttcccatc ttacccaaaa caacgaaccg gcagattact gcgcataact tagtcctgtt
     1261 gtaaaacagg agtttaaatg ccaatcacaa cgattaattg gctaccttgt aactaccaaa
     1321 ccagacggaa atggatcctg caaaaacact ccctcgcccc cgaagccccg ccgacagcgg
     1381 gccaataaga agccgaacat tttactggat ggtgaccaaa tggctcaatc cggaaaattt
     1441 gccaagcact gcaccttgtt tttgctcttt tttacccggc aacctgctac gctgggaata
     1501 ccaaaagtac ttttgaatgc caagagctca cagcttgcga tcacaacaac aaaaaaacta
     1561 aaaaacatga aatcagaaac tttaaacttt ataaagggaa taattatctt tttttttatt
     1621 gcagtcccac taatttcatt tgcccaggaa aaggttacct gggactaccc ggtgaagccg
     1681 gggagcgcgg agtggaaaaa gcttagctca catcaagaaa tggttcaaat ttgtcagatt
     1741 ccagctggaa ccttatcctt tatttctaca aaagaattgg ctcaaatttg tttaaattac
     1801 cctctgtttt tcacaatgaa agcttttaat aatcttcaat taggatttaa tcaagtgtca
     1861 accgaattta atggttttca ggagttattt aaaagggatg atgctggaaa tgtattgttg
     1921 gagttataca aagcaataaa acctcaagta attttgttca aaacaacatc acttgaaaag
     1981 ggaaagcata ttcaaaatat cttctttatt gaatttgttc tttctcaaaa agaaattatt
     2041 gaaaaattca cgaaagcaga aataaaacaa ttgttagagg aatcattggt aaaattggaa
     2101 gagaaaaaac aatatggttt ttcaacattc agcacccaaa caacttctct cgtaatgtgc
     2161 agagttcttg aaaacataaa atttgataaa tacattcaaa taaacacggt atttgaaaaa
     2221 tacaaatatt tctcaaatgc tatcattctt tcggacaaag aaatgattga ggatttaaaa
     2281 aaaataacca ttgactatct taatgataat agtaattgaa aatgaaaaac ttgagtgttt
     2341 ttgccataat aagttttgtt ttgattattt catgcgaaaa ggaaacatta aaatgttgtt
     2401 cctgtataga ggctgaaagc gtttctgaca aatatatttt tccaatcagg ccaggcatgg
     2461 aagaatggaa agaactcaat tcccaccagg cgatggttga ggtatgtcag atacccaaag
     2521 aaatcctgaa cactatgtgt accattggat tggttgatac ttatcttgat tatcccattc
     2581 tgtttacaat ttttgcattt aataacataa acgaaggatt aagccaggca gtaagtgagt
     2641 tcaatggttt tgggcaattg ctgcagaaaa aagattgcgc tacaaaattt ttatcaagat
     2701 acgaaccgat caatccagcc aacatcgacc cttcgtggac aagtatcgag aagggaaggt
     2761 tcatgcaaac tttacgaatt atggaagtaa ccctggcatt tgatccaatg ggaagaaatc
     2821 ttacgaaaga agagcgaaag aaacttattg aaattggaat ggagaaattg aaaataaaag
     2881 aaatgcatga ctatagcggc ccttcgcaaa taacaaacct ataccttatt ggcaacattt
     2941 taaagctcga cgggtaccaa ccatttttag attttctcca acgccgtaac gatataaaag
     3001 cttttttgaa gggagattta aatagccttt attatgttgc ggatggcgat acaattaaaa
     3061 aatacgtaga atctttttta aagcaacatt aaattttata ctatgaataa acatatttta
     3121 gttttaatag tattgctttc aacttgccta atggtaaaag ggcaatatga ttatgagtat
     3181 gagacaacaa tagtacaaac tcctaccgga gtaaatgttg atgcgctgag ttttagatac
     3241 tggatttaca atgattttga tgcaaatgac attgctattg aaaattttaa ttggacaaat
     3301 gggtataatt gcgagataat agaaaattca acaaaatatt ataactgtca tggttatgtg
     3361 tggtataatg ttgaaggcaa tatgtctcaa gttaatttac gctggataaa taatattgat
     3421 tactatggga atccaacgta caatgttcaa aaatattatt cttcaagtta ttcaggcggc
     3481 aatccatctt ataggcaggt aagcacaaat aaccgtgctt accttaaggt ttcttatttt
     3541 cccagagacc attctgcttt aaccacatca aat
//
LOCUS       DAOSDR010000333         3571 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrimonas sp. isolate AMR_MDS_1826
            NODE_9194_length_3571_cov_4.487486, whole genome shotgun sequence.
ACCESSION   DAOSDR010000333 DAOSDR010000000
VERSION     DAOSDR010000333.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863355
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrimonas sp. (wastewater metagenome)
  ORGANISM  Petrimonas sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Dysgonomonadaceae; Petrimonas.
REFERENCE   1  (bases 1 to 3571)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3571)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:10:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,720
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 35
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3571
                     /organism="Petrimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9194_length_3571_cov_4.487486"
                     /isolate="AMR_MDS_1826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2023866"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..763)
                     /locus_tag="PLI30_11385"
     CDS             complement(<1..763)
                     /locus_tag="PLI30_11385"
                     /inference="COORDINATES: protein motif:HMM:NF017390.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M56 family metallopeptidase"
                     /protein_id="HOI80191.1"
                     /translation="METQFFIYLAKVNGALLLFYLLYIAVFRHDTFIRLRRSYLLSAV
                     LFSLLYPLFTVDSLGGLFSILQSRQPVEATIELGEPTALLLESDAAGFTVPWRFIVQS
                     ILLLGSLFFAFRLIMQVVSIVRIRRRCREEVISGYKILNASHEITPFSFFKWIIVNTD
                     AHSEEELQQILIHESTHVRQWHSADVLLGELLSVFFWWNPAVWLLKREIVINLEYLAD
                     NDVLQHGVNSREYQYHLLKLTYHETAVLLSNHFNVS"
     gene            complement(838..1200)
                     /locus_tag="PLI30_11390"
     CDS             complement(838..1200)
                     /locus_tag="PLI30_11390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019538484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HOI80192.1"
                     /translation="MERLTPQEENIMLHVWQIGECAIKDVHDRLEEPKLPYTTVASVF
                     NNLENKGYLNKSRSGNMKVFTPRISEATYKRHFLSGVVKSYFDNSYKELVSFFAKEQK
                     ISSEELEEIIRLIEKKQK"
     gene            complement(1335..2267)
                     /locus_tag="PLI30_11395"
     CDS             complement(1335..2267)
                     /locus_tag="PLI30_11395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008142168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HOI80193.1"
                     /translation="MKFLIATEKPFAPAAVKQIREIIENAGHETVLLEKYTDKNELLH
                     AVSTADAMIVRSDVVDKEVLDAAGNLKIVVRAGAGYDNVDLHAATANNICVMNTPGQN
                     SNAVAELVFGMLLYFARCSFDGSTGSELKGKRLGLHAFGYIGKQVAKIANGFGMEVLA
                     YSPSLALNPEKGKAFNVTAVQSPGELYEKSEIISLHMPANDQTRGSIDYDLLSKLPQK
                     GIVINTARKEVVNEADMLRIMEERPGFKYATDIKPVNHDKMARKFGNRYLASAKKCGA
                     QTSEANVNAGLAAAEQIVKFFADGDETFRVNPIK"
     gene            complement(2387..3454)
                     /gene="serC"
                     /locus_tag="PLI30_11400"
     CDS             complement(2387..3454)
                     /gene="serC"
                     /locus_tag="PLI30_11400"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013548159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004648 -
                     O-phospho-L-serine:2-oxoglutarate aminotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
                     /protein_id="HOI80194.1"
                     /translation="MKIYNFSAGPSILPQSTIENTAEAVKNFNGIGLSILEISHRSKD
                     FQAVIDEAVALFKELLDIPEGYSVLFLGGGASMQFCMVPYNLLEKKAAYLNTGTWASK
                     ALKEAKLFGEIVEVASSKEALFNYIPKDYTVPADADYFHITTNNTIYGTEIRKDIDSP
                     VPLVADMSSDIFSRPLDVSKYGLIYGGAQKNLAPAGVTFVIVKEELLGKVTRPIPTML
                     DYRTHIKEGSMFNTPPVLPIYSAMETLRWLKGLGGLAAMEKINLEKAALLYDAIDSSK
                     IFTGTAKEEDRSIMNVCFVMKDEYKELEPEFQKFATERGMSGIKGHRSIGGFRASIYN
                     AMPKSGVQALVDCMKEFERNH"
BASE COUNT          943 a          773 c          798 g         1057 t
ORIGIN      
        1 gtgatacatt aaagtgatta cttaacagaa cggccgtttc gtgataagtc agtttgagca
       61 ggtggtattg gtattccctg ctgtttacac cgtgttgaag cacatcgtta tcggccaggt
      121 attcgaggtt gatgacgatt tcgcgcttca acagccagac tgccggattc caccagaaaa
      181 agacggaaag caactcgccc aacaacacat ctgccgaatg ccattggcgg acatgggtgc
      241 tttcgtggat gaggatctgc tgtagctctt cttcggagtg tgcatccgta tttacgatta
      301 tccatttgaa aaacgagaag ggagttattt cgtgtgaagc gtttaggatt ttgtatcccg
      361 aaataacctc ttcccggcac cggcgccgga tccggacaat ggaaacgact tgcatgatca
      421 accggaaagc aaaaaaaagg cttcctaaaa gaagtatgct ttgaacaata aacctccatg
      481 gaaccgtaaa tccggcagcg tcactttcca gcagaagagc ggtaggttca ccgagttcaa
      541 tagtcgcttc cacgggttga cgtgactgca agattgaaaa taatccaccc agggaatcta
      601 ctgtaaaaag aggataaagc aacgaaaaca gaacagccga caataggtac gacctccgta
      661 gccggatgaa cgtatcgtgc cgaaaaaccg caatatacag caggtaaaaa agcagcaaag
      721 ctccgtttac tttggccagg tatatgaaaa attgcgtttc cattttgcgt ttgatttcag
      781 acttcagagc acagacacga gacggttagg agcgaagcgg tctgttatct gatgaagcta
      841 tttctgtttt ttctcaatca gacgaataat ttcttccagt tcctcggacg aaattttctg
      901 ctccttggca aagaacgaca ccagttcttt atacgaatta tcgaaatagc ttttcactac
      961 tcccgacaga aaatgtcttt tgtacgtcgc ttccgagatc cttggggtga aaactttcat
     1021 gttgccggaa cgtgatttat tgagataacc cttgttctca aggttgttga atactgaagc
     1081 cacggtggtg tagggtaatt taggctcctc gagcctgtcg tggacatctt ttatggcaca
     1141 ttcgccaatt tgccacacgt ggagcataat gttttcttcc tgaggtgtta atctttccat
     1201 atctttttca attgataaat ttactacgat acaaaagtac gaatatatcg taaataaaga
     1261 caactttttg aattatttat ggttaaaaaa ataaaaatcc cgataaaaat tcgttatcgg
     1321 gatataggtt gcttttattt tatcggattt accctgaacg tttcatctcc atcggcaaag
     1381 aattttacga tttgttctgc tgcggcaagc cctgcgttta cgttggcttc actggtttgt
     1441 gccccgcatt ttttggcaga tgccaggtag cggttaccaa actttcgcgc cattttatcg
     1501 tgattcaccg gtttaatatc ggtagcgtat ttgaaccccg gccgttcttc catgatacgt
     1561 aacatgtcgg cttcgtttac cacctctttg cgtgcagtat tgatcacgat gcctttttgc
     1621 ggtaacttgc tcaataaatc gtaatcaata ctacccctgg tttggtcgtt tgccggcatg
     1681 tggagcgata tgatttcact tttctcatac aactcaccgg gcgattgcac agcggttacg
     1741 ttgaatgctt ttcccttttc aggattgagg gccagcgaag gggagtaggc caacacttcc
     1801 attccgaagc cgttggcgat ctttgcgacc tgttttccaa tgtatccgaa agcgtgaaga
     1861 cccagtcttt tccctttgag ttccgaaccg gtagatccgt caaaactgca acgggcaaaa
     1921 tagagaagca taccgaaaac cagttcggct acagcattgg agttttgccc gggtgtattc
     1981 atcacgcaaa tattgttggc ggtggctgca tgaagatcaa cgttgtcgta tccggctccg
     2041 gcgcggacga caattttcag gtttccggcc gcatccagta cttctttatc gacaacgtca
     2101 ctgcggacaa tcatcgcatc tgcggttgat acagcatgta gaagttcgtt tttgtccgtg
     2161 tatttctcca gcaaaacagt ttcatggcct gcattttcaa taatttccct gatttgttta
     2221 actgcagctg gcgcaaaggg tttttcggtt gcaattaaaa atttcataat agttgtggtt
     2281 taataagtct gtgattaaat taaaaaacgc ggggataggt ttcaacgtac cggataaaag
     2341 gttttgatta tccggtacgt taattgtgtg gctttacaag aaacaactaa tggttccttt
     2401 cgaattcttt catgcagtca accagggctt gtacgcctga ttttggcatt gcgttgtaga
     2461 ttgacgcgcg gaaccctccg attgagcggt gtcccttgat gcccgacatt cctctttcag
     2521 tagcaaattt ctggaactcg ggttcaagtt ctttgtattc gtctttcatc acgaagcata
     2581 cgttcatgat ggagcggtct tcttcttttg ccgttccagt aaatatttta ctgctgtcga
     2641 tggcatcgta cagcagggct gctttttcga gattgatctt ttccattgct gccagacctc
     2701 ccaggccttt cagccaacga agagtttcca tcgctgaata aataggcaat acgggaggcg
     2761 tattgaacat ggagccttct ttgatgtgtg tgcgatagtc cagcatggtc ggaatcggac
     2821 gggtgacttt tcccaagagt tcttccttta caataacaaa ggtgacacca gccggtgcca
     2881 ggttcttttg tgctccgccg taaattaaac cgtacttgga tacatcgagc ggacgggaga
     2941 aaatatcgga agacatatcg gcaaccaggg gtaccgggct gtcaatatct ttccggatct
     3001 cggttccgta gatggtgtta ttggtggtaa tgtgaaaata atcagcatcc gcaggaacag
     3061 tataatcttt tggaatatag ttgaaaagcg cttccttgga agaagcaacc tctactatct
     3121 ccccaaacag tttagcctcc ttaagtgctt tgctggccca tgttcctgta ttcaggtagg
     3181 ctgccttttt ctccaacagg ttatagggaa ccatacagaa ctgcatgctt gcacctccac
     3241 cgaggaacag tactgagtat ccttcgggaa tgtctaacag ttccttaaaa agagccacag
     3301 cctcgtcaat aactgcctga aaatctttgg accggtgact gatttcaaga atggacaggc
     3361 cgattccatt gaaatttttc acagcttctg ccgtgttctc aattgtcgac tgcggtaaaa
     3421 tagatggacc ggcgctaaaa ttataaattt tcataaaaaa taatttttaa tgttttgtat
     3481 ttaaaggtta tgtggtttaa gtttcaatca aattcagaac aaagatagaa gtttatttgt
     3541 aagtttcaat gcgaaaagct gaaaataatt t
//
LOCUS       DAOSDR010000334         3556 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrimonas sp. isolate AMR_MDS_1826
            NODE_9248_length_3556_cov_2.463867, whole genome shotgun sequence.
ACCESSION   DAOSDR010000334 DAOSDR010000000
VERSION     DAOSDR010000334.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863355
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrimonas sp. (wastewater metagenome)
  ORGANISM  Petrimonas sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Dysgonomonadaceae; Petrimonas.
REFERENCE   1  (bases 1 to 3556)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3556)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:10:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,720
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 35
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3556
                     /organism="Petrimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9248_length_3556_cov_2.463867"
                     /isolate="AMR_MDS_1826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2023866"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1321)
                     /locus_tag="PLI30_11405"
     CDS             complement(<1..1321)
                     /locus_tag="PLI30_11405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008658220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HOI80195.1"
                     /translation="MKKKNIIYIVAAILAFSSCNDFLDKSPLDTFTNDNFWTNESNVT
                     GYANTFYQDFLGYGNAGGSGLFYFRTLSDDQAGGSFADWTFQNVPASSTNWRDGWIEI
                     RRANILLENVDKVNMSDEAKNHWRGVGRLMRAWHYYQLVRAYGDVPWVDKSLDITDEG
                     YLYGVRENRDAVMDHVLEDLNFACNNMNDNNSKIKLNKNVANAMKAEITLFEGSFRKY
                     RKAEDGQSAPDMAGAGKFFTETKTAAAFLMGKNFTLSPTYQGNYNSVNLADNPEMIFY
                     KAYKQNVQHHSLIAYISSSTQISGMSKNAFESYLFTDGKPLSLTSLDKSDAAVMKIGT
                     KTVGGNVVPDTVMSIKKILDMRDKRLSQTIDTALCYVGRGFTRFGTGISMTSSTGYGV
                     NKYDNKSLDNMYRNQTSANFTHAPIFWLSVVYLQYAEACAELGSITQD"
     gene            complement(1353..>3249)
                     /locus_tag="PLI30_11410"
     CDS             complement(1353..>3249)
                     /locus_tag="PLI30_11410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009291443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0071702 - organic substance transport
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SusC/RagA family TonB-linked outer membrane
                     protein"
                     /protein_id="HOI80196.1"
                     /translation="DNNSFIRMGTFAKAILAKGLTLNADYTYNVNNYTYKAAYIPLGG
                     WNSWGGAIEKTTLYTSSSWLEQQSDQDKTFALNIYGNYEFSLPYRHHFNLMLGGNAEG
                     NTYFNHYSERNDLLDYNLPEFDLATGSQYVDGAHSHWATAGYFGRLNYNYDGKWLLEL
                     NGRYDGSSRFPVNDRWAFFKSASAGYRISEEAFFDPLKESINNLKLRASYGEIGNQAV
                     GSNMYLSTLTKRDASNKPADDVKWLTDEGVPVVAYNMPKLVSSTLKWERIQTLDFGAD
                     LGLFNNELNFTFDWYQRTTKDMLAPGQTMPQVLGATAPYVNAGTLRTRGWELTIDWRH
                     RFNDVLLYANANVGDFVTDIVKWDNETKLLNQNYSGKRYGDIWGFETDRFFTEEDFNA
                     DGTYKTGIASQVGLQQGTFVFGPGDIKFKDLDGNEIIDGGKGTADDHGDLKVIGNTTP
                     RYQYSFRLGAEWRGFDVDMFFQGVGKRSVWTQSAFVMPMMRGADAIYENQTNYWTEEN
                     PDFKADFPKLFPGNAGRGTIAVLELGNHNFYPQTKYIVNMAYLRFKNLTLGYTLPTDM
                     ASRIYMQRARIYFSANNLMELINKSNAPVDPEVNDKEEGVSLGNATWGRIDPMYRTIS
                     FGVQLTF"
     assembly_gap    3250..3259
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3260..>3556)
                     /locus_tag="PLI30_11415"
     CDS             complement(<3260..>3556)
                     /locus_tag="PLI30_11415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009291443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI80197.1"
                     /translation="TSGKTSYYMSVGYNHEEGVMTYFPEKMDKWTAMLNVTTDVKDWL
                     QIGARFNYSNKDFIGPATRRTTYQYMWRWGSFFGPYGTYQGTDFRNDIAYRKQAG"
BASE COUNT          883 a          825 c          755 g         1083 t
ORIGIN      
        1 cgtcttgggt gattgaacct aactctgcac aagcttccgc atattgtaaa tatacaacag
       61 ataaccagaa tataggagcg tgggtaaaat tagcactggt ttggttcctg tacatattat
      121 ccagactttt gttgtcatat ttgtttaccc cgtatcctgt ggatgacgtc attgaaatgc
      181 cggttccaaa ccgggtgaat ccccgtccca catagcatag tgccgtgtcg atggtttgac
      241 tgagccgctt atcacgcata tccaatatct ttttaatgga cattaccgta tcgggaacta
      301 catttcctcc tacagtttta gtccctatct tcattactgc ggcatccgat ttatccagag
      361 atgtcagtga caatggttta ccgtcggtga aaagataaga ttcgaatgca ttcttgctca
      421 tgccgcttat ttgggttgat gagctgatat aggcaatcag ggagtgatgc tgcacatttt
      481 gtttgtaggc tttgtaaaaa atcatttcag gattgtcagc cagattaacg gaattgtaat
      541 tcccctggta agtaggactt aacgtgaaat ttttgcccat caggaatgcg gctgcggttt
      601 tcgtttccgt gaagaatttg cctgcgcccg ccatatcggg ggcagactga ccatcttctg
      661 ctttacggta tttacggaaa ctgccttcga aaagcgtgat ttcggctttc atggcgttgg
      721 cgacattttt attcaatttt attttggaat tattatcgtt catattgttg caagcgaaat
      781 ttaaatcttc caacacgtga tccatcactg catcccgatt ttcccggaca ccgtataaat
      841 acccttcgtc tgtgatgtcc aatgatttat ccacccaggg gacatcccca taggcgcgta
      901 caagctggta ataatgccag gctctcatta accgaccaac gcctctccag tgatttttag
      961 cttcatcgct catattcact ttgtccacat tctcaagtag gatattggcc cgtcggattt
     1021 caatccatcc atcccgccag ttggtgctgc tggccggcac gttttgaaat gtccagtcgg
     1081 caaaacttcc gcctgcctgg tcatcggaaa gggtccgaaa ataaaagaga cccgaccctc
     1141 cggcattccc ataccctagg aaatcctggt agaaggtatt ggcgtaaccg gttacattac
     1201 tttcatttgt ccagaagtta tcgttggtaa aagtatccaa cgggcttttg tccagaaagt
     1261 cgttacaaga agagaaggcc aagattgcag caacgatgta tattatattt tttttcttca
     1321 tatttctgtt tttttataat gaatacaaca gtttagaatg tgagctgaac accaaatgat
     1381 attgtcctgt acatggggtc tatacgtccc caggtcgcat tacccagact tacaccttct
     1441 tctttgtcat tgacttcggg atcgacgggc gcgttactct tgttaatcag ctccatcagg
     1501 ttatttgcac tgaaatagat cctggctctt tgcatatata ttctcgatgc catatcggta
     1561 ggaagcgtgt aacctagtgt taggttcttg aaacgcagat aggccatgtt cacaatatat
     1621 ttagtctgag ggtaaaaatt atggttccct aattccagaa cagcaatcgt acctcttccg
     1681 gcattacccg ggaataattt cgggaaatcg gctttgaaat cgggattttc ttccgtccag
     1741 taatttgtct ggttttcata aatggcgtca gcacctcgca tcatcggcat gacaaatgca
     1801 ctctgtgtcc acaccgagcg ttttccaact ccctggaaaa acatatccac atcgaatcct
     1861 ctccattcag cacccaagcg aaaactgtac tggtaacgcg gagtggtgtt gccaatcact
     1921 ttcagatcgc cgtggtcatc ggcagttcct tttccaccgt caataatttc gtttccatct
     1981 aaatccttga acttgatgtc accggggcca aatacaaatg ttccttgttg taatccgact
     2041 tgagaggcaa tccctgtctt ataggtgccg tctgcattaa aatcttcctc tgtaaaaaaa
     2101 cgatctgtct cgaatcccca gatgtcgcca taccttttac cactgtaatt ctggtttaat
     2161 aatttggttt cattgtccca tttcacaatg tctgtgacaa aatctcccac attagcattg
     2221 gcatatagca atacatcgtt gaagcggtgg cgccagtcaa tcgtcaattc ccatccacgt
     2281 gtgcgaagag ttcctgcgtt gacatagggt gcagtggctc cgagtacctg tggcattgtt
     2341 tggcctggtg cgagcatgtc tttcgttgta cgttgatacc aatcgaatgt gaaattcagt
     2401 tcgttgttaa agagccctaa gtcggcacca aaatcgagcg tttgtatacg ctcccatttt
     2461 aatgtggagg aaaccagttt gggcatattg tatgcaacaa caggcacacc ttcatcagtt
     2521 aaccacttta cgtcatcagc aggcttgttg gatgcatcgc gttttgtcag tgtggaaagg
     2581 tacatgttgc tacctactgc ctgattgccg atttctccgt atgaggctct taacttgaga
     2641 ttattgatcg attcttttaa aggatcgaag aaagcctctt cgctgatcct gtatccggct
     2701 gatgccgatt tgaaaaaagc ccagcggtcg ttaaccggaa accgggagga gccatcataa
     2761 cgtccgttta actcgagcag ccactttccg tcatagttgt agttcaagcg gccaaagtat
     2821 ccggctgtag cccaatgcga atgtgccccg tcaacgtact ggctgcctgt ggccaggtca
     2881 aattccggca gattatagtc tagcaggtca tttctttcag aataatggtt gaagtatgta
     2941 tttccttccg catttcctcc caacataaga ttgaaatggt ggcgataggg taaagaaaat
     3001 tcataattac catagatatt cagtgcaaag gtcttgtcct gatcggattg ttgttccagc
     3061 caggagctac tggtatacaa cgttgtcttt tcgatggctc ctccccagga attccatcca
     3121 cccaaaggta tgtaggccgc tttataggta tagttattca cgttataggt atagtcggca
     3181 tttagggtta atccttttgc cagaattgcc tttgcaaaag ttcccatacg tatgaaacta
     3241 ttattatcgn nnnnnnnnng cccgcttgct tacggtaagc aatgtcgtta cggaagtcgg
     3301 taccctgata agtcccataa gggccgaaga aacttcccca gcgccacatg tactgatagg
     3361 tcgttctgcg tgtggcaggt ccaataaaat ctttattgct gtaattgaac cgggctccga
     3421 tctgtaacca atctttcaca tcggttgtta cgttcaacat agctgtccac ttgtccattt
     3481 tttcggggaa atatgtcatg acaccttctt cgtggttgta tcctaccgac atgtagtagg
     3541 acgtttttcc acttgt
//
LOCUS       DAOSDR010000335         3538 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrimonas sp. isolate AMR_MDS_1826
            NODE_9316_length_3538_cov_3.159345, whole genome shotgun sequence.
ACCESSION   DAOSDR010000335 DAOSDR010000000
VERSION     DAOSDR010000335.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863355
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrimonas sp. (wastewater metagenome)
  ORGANISM  Petrimonas sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Dysgonomonadaceae; Petrimonas.
REFERENCE   1  (bases 1 to 3538)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3538)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:10:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,720
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 35
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3538
                     /organism="Petrimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9316_length_3538_cov_3.159345"
                     /isolate="AMR_MDS_1826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2023866"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..626)
                     /locus_tag="PLI30_11420"
     CDS             complement(<1..626)
                     /locus_tag="PLI30_11420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006842273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HOI80198.1"
                     /translation="MRIVDTHAHLYLEDFEKDLDDVVARARSIGVEKVLLPNIDETTV
                     SDLKQTVDKYPSFFIPMMGLHPTNVREDWEQQLEIIYDELSRPGYIAIGEIGIDLYWD
                     KSSKERQVAAFERQLTWSIEKKLPVAIHSREAIPEVIESIKKTGEKDLFGVFHSFGGS
                     IGELKEILELGNFYIGINGVVTFKKSGLMETLENCVLENIILETDSPYL"
     gene            complement(623..1318)
                     /locus_tag="PLI30_11425"
     CDS             complement(623..1318)
                     /locus_tag="PLI30_11425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019537753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI80199.1"
                     /translation="MPYRRLPNTDLSRIKALKTAIEKAAGTDFQDVAISMKTLSRARS
                     VVEKFERLSLKYQQTLDTQVKANIAFQSKVKNARMYLSHFIQVLYMSVMRSEIKPEHL
                     ELYGLQDTNFVVPDLSSNEQLLQWGQRIIAGENKRVARGGVPIYNPGIAKVSVMYILF
                     KEGYQTQQIHQKATARTLDEVSEFRSEVDGVIFDIWEEVERFNAELPGNERLDKNREY
                     GLIYYYRKGETIG"
     gene            complement(1328..2308)
                     /locus_tag="PLI30_11430"
     CDS             complement(1328..2308)
                     /locus_tag="PLI30_11430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007766784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HOI80200.1"
                     /translation="MILHSADTLSKKIEEAIQNISFDLKPASLFEPISYSLSVGGKRV
                     RPLLALLATDMFGKEVDQSLKPAIGIEIFHNFTLLHDDLMDNSDMRRGMPAVHKKWDT
                     NTAILSGDAMLIESYKYVSSVPPAVLPEILDLFSKTAMEVCQGQQLDMDFEKRTDVTE
                     SEYLEMIVLKTAVLLACSLKMGALLAGASPRDAELLYTFGINIGLAFQLKDDLLDVYG
                     DVKTFGKNIGGDIVSNKKTYLLIKALKTSGERQRTELLKWITAEKFDKNEKIESVKNI
                     YNDLNLKSISENLIEKYYLAALDSLSLVHVADERKKELISLSENLMYREK"
     gene            complement(2390..3085)
                     /locus_tag="PLI30_11435"
     CDS             complement(2390..3085)
                     /locus_tag="PLI30_11435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019537755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HOI80201.1"
                     /translation="MNDEVKKSPKANIETLKLTSILMGMVVGVALLFFAFEWSSETKK
                     LDESVIVQDVLAEEEIEITRRDPTPPPPPPPPAPETPEIIQVVEEKVETRIEINTEDD
                     ASKRQMEAFVPPPPPKPKVEEVTEEIFVVVEEQPEFPGGNTAMMKFLSDNIKYPVIAQ
                     ENGIQGRVITNFVVERDGSITDVQVVRGVDPSLDKEAIRVIQSMPKWKAGRQRGSAVR
                     VRFTLPVVFRLQQ"
     gene            complement(3455..>3538)
                     /locus_tag="PLI30_11440"
     CDS             complement(3455..>3538)
                     /locus_tag="PLI30_11440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006800094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HOI80202.1"
                     /translation="PNDFIVGYGLDYDGYGRNLKDIYKIRP"
BASE COUNT          842 a          840 c          743 g         1113 t
ORIGIN      
        1 agataggggg agtcggtttc gaggatgatg ttctccaaca cacaattttc cagtgtttcc
       61 atcaatcccg attttttgaa ggtgaccact ccgtttatcc cgatgtaaaa attcccaagt
      121 tccaggatct ctttcagctc gcctatgctt cctccaaaac tgtggaagac tccgaaaagg
      181 tctttttctc cggttttttt tatcgattct atcacttccg ggatagcctc tcttgaatga
      241 atggctaccg gcagtttttt ttcaatgctc caggtgagct gcctttcaaa agcggctacc
      301 tgtctttctt ttgagctttt atcccaatac aagtcgatac ctatctcacc gatggctata
      361 tatcccgggc ggcttagctc gtcgtaaatg atttccagct gttgttccca gtcctcccgc
      421 acgtttgtag gatgcaaccc catcatcggt ataaaaaacg acgggtattt atcgacggtt
      481 tgctttaaat cagaaacggt tgtttcgtct atgttgggaa gcagcacttt ctctactcct
      541 atcgacctgg ctctggcaac tacatcgtcc aggtcttttt caaaatcttc cagatacagg
      601 tgggcgtgcg tatcaacgat tctcatccaa tggtttctcc tttgcggtaa taatagatca
      661 gaccgtattc cctgttttta tccagccgct cgtttcccgg aagttcagca ttaaatctct
      721 ccacctcttc ccagatatca aaaataacac cgtccacctc acttctgaac tcggaaactt
      781 catccagggt tcttgcggtg gctttctgat ggatttgctg ggtttggtaa ccctctttaa
      841 acagaatata catgacactc actttggcaa tgcccggatt gtatatcggc acaccacccc
      901 gcgccacccg cttgttctct ccggcaatga ttctctggcc ccattgcagc agttgttcgt
      961 ttgagctcag gtcgggaacg acaaagtttg tgtcctgcaa accgtaaagc tccaggtgtt
     1021 ccggttttat ctcggagcgc atgacgctca tgtacaagac ctgaatgaaa tgggagaggt
     1081 acatcctcgc attcttcacc ttgctttgaa aggcaatgtt cgcttttacc tgggtatcca
     1141 gggtctgctg gtatttcagg gaaagacgtt cgaatttttc caccacgctt cttgccctcg
     1201 acagtgtttt catggatatg gcgacatctt gaaaatcagt accggctgct ttctcgatag
     1261 ctgttttcaa cgctttaatc cgcgacaggt ctgtattggg taatctgcga tagggcattt
     1321 agggaaatta tttttctctg tacatcaggt tttccgacaa ggaaatgagt tcttttttcc
     1381 tttcgtcagc cacatgcacc aacgaaagag aatccaacgc tgcaagataa tacttttcga
     1441 tgagattttc cgaaatcgac tttaaattta aatcattgta aatattttta acggattcaa
     1501 ttttttcgtt tttgtcgaat ttttctgcgg taatccattt tagtaactcc gtgcgttgcc
     1561 tttcgccgga tgttttcagc gcttttatca gcaggtaggt ttttttattg ctgacaatgt
     1621 ctccgccgat atttttccca aacgttttaa catctccgta aacatccaac aaatcatctt
     1681 tcagttgaaa agccagtccg atgtttatgc caaaggtgta gagcagttcc gcatcacgcg
     1741 gggaggctcc ggccagcaag gctcccatct tgagcgaaca ggctagaagt accgccgtct
     1801 ttagcacgat catctccagg tactccgact ctgtgacatc tgttcttttc tcgaaatcca
     1861 tatccagctg ttgtccctga cacacctcca tggccgtttt tgaaaagagg tcaaggattt
     1921 ccggcaggac agcgggtggc acggaactta cgtatttata cgattctatc agcatggcgt
     1981 cacccgagag aatggcggtg ttggtatccc acttcttgtg gaccgccggc atgcctctcc
     2041 gcatatccga attgtccatc aggtcgtcgt gcaacagggt gaagttgtga aaaatctcta
     2101 tccctatggc cggcttcaac gattggtcca cctccttgcc aaacatatcc gtggctagca
     2161 aggccaacaa tgggcgtacc ctctttcccc caacggagag ggaatagctg ataggttcga
     2221 aaaggctcgc tggttttaaa tcgaaagaga tattttggat ggcttcctca attttcttgc
     2281 ttaaggtgtc ggcgctgtgt aaaatcatgt tggtgaaatt taatttagaa caaaaagctg
     2341 cctttgggac aaaggcagct ttcgatattt caagaattac tgtagttgat tattgttgca
     2401 ggcggaacac cacaggaagg gtgaatcgta cgcgcactgc agagccacgt tgccttccgg
     2461 ctttccattt gggcatcgac tggataacgc ggatagcctc tttatcgagt gatgggtcaa
     2521 ctccgcgaac tacctgaacg tcagtaatac ttccgtctct ctctaccaca aaattggtga
     2581 tgacgcgtcc ctggatgccg ttttcctgag caattaccgg gtatttaatg ttgtcgctta
     2641 agaacttcat catggctgta tttcctcccg ggaattccgg ttgttcttct accacaacga
     2701 aaatttcttc ggttacttct tctactttag gtttgggtgg tggtggcgga acaaaagctt
     2761 ccatctgcct cttacttgca tcatcctccg tgttgatctc gattcttgtt tccacttttt
     2821 cttccactac ctgaatgatt tccggagttt ccggtgccgg tggcggtggt ggcggtggcg
     2881 gagtcggatc gcgacgagtg atttcaattt cttcttctgc caaaacgtcc tgaacgataa
     2941 cactctcgtc cagttttttg gtttctgaag accattcaaa agcgaaaaat aacaacgcta
     3001 ctcccactac catacccatc aggatggaag tcagcttgag cgtttctatg ttcgctttgg
     3061 gtgatttttt tacttcatca ttcattttga ttctactttt ttatgtgttt tactgactga
     3121 atatacccac gacatcgggt gcaaaaatag gcaaattttt ttatactgtc gtatttcgcc
     3181 gataaaattc tcaaaaaaat gtcattttct cgctagattg accaaatccc gttacaaata
     3241 tcgttttaat tagcgtattt gaattcacct caaatgtaca aattagacgg caaaatcggc
     3301 gttttgttta gctaataaat atcaaaatgc aagcttgttt ttgattttaa cattaaatag
     3361 acgtaaaatg ggctggtaaa cgattaatat atttcaattt taacgtttcg ttaggaatat
     3421 tttgaatcaa gccgttacgg gcgatgccgc attatcaggg cctgatcttg taaatgtcct
     3481 ttaagtttct tccgtaaccg tcgtagtcca ggccatagcc cacgataaaa tcgttggg
//
LOCUS       DAOSDR010000336         3534 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrimonas sp. isolate AMR_MDS_1826
            NODE_9322_length_3534_cov_2.875826, whole genome shotgun sequence.
ACCESSION   DAOSDR010000336 DAOSDR010000000
VERSION     DAOSDR010000336.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863355
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrimonas sp. (wastewater metagenome)
  ORGANISM  Petrimonas sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Dysgonomonadaceae; Petrimonas.
REFERENCE   1  (bases 1 to 3534)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3534)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:10:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,720
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 35
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3534
                     /organism="Petrimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9322_length_3534_cov_2.875826"
                     /isolate="AMR_MDS_1826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2023866"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..434)
                     /locus_tag="PLI30_11445"
     CDS             complement(<1..434)
                     /locus_tag="PLI30_11445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI80203.1"
                     /translation="MNKFGIALFLLFFFFLQTACSEGDDPEENEIPISEDSYPGLSVS
                     PTGQLLLNGEPFYGIGVNYFNAFTRTLEQGKENDKSYEIGFAYLKERNIPFIRFSLNG
                     YWPRDWDTYKNNPDRFYKNLDAFVKAAEEYGIGLIPSFFWHNP"
     gene            complement(508..2382)
                     /locus_tag="PLI30_11450"
     CDS             complement(508..2382)
                     /locus_tag="PLI30_11450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007572565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HOI80204.1"
                     /translation="MKNIYILIIALITLFLNSCNDVLDQVPSDRYTDALVWNDPYLLD
                     THLAELYACTPVMIQDAITLMNSWNGAPMNRDDNGWTAIMGFSAQMEGTVRTLEITDE
                     AKYNFGGQAHLLSMKENGIQSNGTELQWRGNAYYTIRLLNNFIDRAGESSISNVEERI
                     AEARFLRAFNYFAMAKRYGGVPLITKVQSLDDDEDVLYPKRATEKEIFDFILKETDEI
                     SEVLPSANDAGRANKWAALALRSRAALYAASVAQFGTQQLNGLLGFPAGDDAYYYQIC
                     FDASKKIMTEGPFMLYDEDADKIQNFKNIFLKKGNSEAIMVKQHSGKGFNDGGTNRWS
                     WDMVECPRPNVWGVGNYHGPYLDLVEEFEYKDGTPGVIDREYAKNKLWTMEELWGEKD
                     PRFFASIWTNGTSWQGAVGGVLGENTIDMHNGIMTAEGTLVDGRYDTYNGIPAVGDQL
                     VRFKEGNTPNTGFGVMKYLDPNANNFNWFSESTTDYLIFRYGEILLNFAEAAFELGKP
                     AEALDAINQIRDRAGIARLTSVDREKIRHERKVELAFENHRYWDLRRWRIAVQSLTRT
                     YSGLRYIYDAASSKYRLDFIDNIDGRAKTPTFPEKNYYFPIGQGRIAANPNLVENPGY
                     "
     gene            complement(2395..>3534)
                     /locus_tag="PLI30_11455"
     CDS             complement(2395..>3534)
                     /locus_tag="PLI30_11455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019538984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SusC/RagA family TonB-linked outer membrane
                     protein"
                     /protein_id="HOI80205.1"
                     /translation="SYGSSGYDAVANFAYLAGYAYDATYTIGNELISALVPTGLPNIN
                     LTWETMKIYNAGVDFSLLNRKLYGEVEFFYRIRSGIPGSRTKSLPSTFGAELPVENLN
                     SQSAKGFELRLGTAGKLGNLTYDITGNIAQSRSKWVDIDEPTYTDPDEIRMYKNSGRY
                     TDRRYGYVFDGLFTSQEEINEWPYTFETLNNDNSSLRPGDVKYKDLNGDGVINWRDQR
                     EIGKGAVPHWTYGLNMLFKYNNFDFSALFQGAFDYTTYIDLEGAPTSVFYSNYWHSER
                     NNSSTALVPRPSGSSTNWFYSDYRNHNTAYLRLKNMSLGYELPNLLLSEIGINRLRIY
                     VAGTNLFTLSTLDKFGIDPEMPEGYGIGIYYPQQFTMSVGCNLTF"
BASE COUNT         1037 a          807 c          641 g         1049 t
ORIGIN      
        1 ggattatgcc agaaaaagga agggataagc ccgatgccat attcttccgc tgctttcaca
       61 aaagcatcca gatttttata gaacctgtcg gggttatttt tgtaggtatc ccaatctctt
      121 ggccaatagc cattcagtga aaagcgaata aacggaatat tgcgttcttt cagataggca
      181 aaaccaatct catacgattt gtcattctcc ttcccttgct ccagggtacg cgtaaatgca
      241 ttgaagtaat tcacgccaat cccataaaat ggttctccat tcaataataa ttgtcctgtt
      301 ggagagacgg agagtccagg ataggaatct tcgctgattg ggatttcatt ttcttccgga
      361 tcatctccct cactacaagc ggtttgcaaa aaaaagaaga aaagtagaaa gagagctatt
      421 ccaaatttat tcatatcaat ttcaaatcaa aaaggaccga cgttcatcaa tccattcatc
      481 cggttgatga acgtcggaaa gtttatatta ataacctgga ttttcaacta gatttggatt
      541 tgcggcaata cgaccctgtc caatcgggaa atagtaattt ttttccggga aagtgggtgt
      601 tttggctctt ccatcaatat tatcaataaa atcaagtcgg tatttactag aagcagcgtc
      661 ataaatgtaa cgtaatcctg aatatgtacg tgttaaggat tgcacagcga ttctccaacg
      721 tctcagatcc caatatctgt ggttctcaaa tgccaactca acttttcgct cgtgtctgat
      781 tttctctcta tcgaccgagg tgagccttgc aatgcctgcg cgatcacgta tctggttgat
      841 tgcatctaaa gcttcagccg gttttcccaa ctcgaaagca gcttcagcga agtttagcaa
      901 aatctcacca taacggaaaa tcagataatc agttgtcgat tcagaaaacc aattgaaatt
      961 gtttgcattg ggatccaggt acttcatcac accaaagcct gtattggggg tatttccttc
     1021 tttgaagcga accaactgat cccccacagc aggaattcca ttataggtat cataacgacc
     1081 atcgacaagg gttccttcag ctgtcatgat tccattgtgc atgtcaattg tattctctcc
     1141 caatacacct ccaactgctc cttgccatga tgttccgtta gtccatatgg aagcaaaaaa
     1201 acgtggatct ttttcgcccc acagttcttc catggtccat aatttatttt ttgcatattc
     1261 cctatcaatg acacctggag ttccatcctt gtattcaaat tcttcaacaa gatccagata
     1321 agggccatga taatttccga caccccaaac atttggacgc ggacattcta ccatatccca
     1381 agaccatctg ttggtgccac catcattgaa acccttgcct gaatgctgtt tcaccatgat
     1441 agcttcactg ttcccctttt ttaagaatat atttttaaag ttctgtattt tatcagcatc
     1501 ttcgtcatac agcatgaaag ggccttcggt catgatcttt ttgctggcat caaaacagat
     1561 ttgataataa taggcatcat caccagcggg gaagcccaat aaaccattta gttgttgagt
     1621 gccaaactgg gcaacactgg cggcatacaa tgcagctctg cttcgcagag ccaatgctgc
     1681 ccacttgtta gctcgtccgg catcattggc tgaaggaaga acttcagata tctcatccgt
     1741 ttccttaagg ataaaatcaa aaatctcttt ttcagtagct cttttgggat acaatacatc
     1801 ttcatcatca tccaaagact gtactttggt aatcaaagga acacctccat agcgtttggc
     1861 catggcaaaa tagttaaatg cccgtaagaa acgtgcttct gcaatccgtt cttccacgtt
     1921 tgagatcgaa gattcgccgg cacgatcaat aaagttattc aacaaacgga tggtataata
     1981 cgcattaccc ctccactgta gttctgtccc atttgattga atgccatttt ccttcatact
     2041 tagcaagtgt gcctgtccac caaaattgta tttggcttca tcagtaattt caagggttct
     2101 gacagttcct tccatttgag cgctaaaccc cataatagca gtccagccgt tgtcatcccg
     2161 gttcatagga gcaccattcc atgaattcat taaagtaatg gcgtcttgaa tcattaccgg
     2221 cgtacatgca taaagttcgg ccaagtgcgt atccaagaga taaggatcgt tccataccag
     2281 tgcatctgtg tacctgtcgc tcggcacctg gtccagtaca tcattacagg aattcaaaaa
     2341 taatgtaatt aacgcaataa ttaatatata tatatttttc ataacttaaa ttctttaaaa
     2401 agtaagatta caaccgacac tcatcgtaaa ttgctgtgga taataaattc caataccgta
     2461 tccttctggc atctcggggt caatgccaaa cttatcaaga gtacttaagg taaacaggtt
     2521 cgtgcctgcc acataaatcc ttaatctgtt aataccaatt tctgacagca ataagttggg
     2581 cagttcataa ccaagagaca tgtttttcag acgcagatac gctgtgttgt ggtttctgta
     2641 gtcggagtaa aaccaattcg tggatgatcc cgaaggcctc ggaaccaaag ccgtagatga
     2701 gttatttctc tctgaatgcc agtaattact gtagaaaaca gaagtagggg ctccttccag
     2761 gtcgatgtaa gttgtataat caaacgcacc ctggaagaga gcagaaaaat caaaattgtt
     2821 gtatttaaag agcatattta aaccgtaagt ccaatgagga acagcacctt tcccaatttc
     2881 tctctggtca cgccaattaa tcactccatc tccatttaaa tctttatact taacatctcc
     2941 cggtctcagg gaagaattat cattattcag cgtctcaaat gtataaggcc attcatttat
     3001 ctcttcctgg ctggtaaata atccatcaaa cacataaccg taacgcctgt ccgtatacct
     3061 tccgctattt ttgtacatcc taatctcatc cgggtcagta taggtaggtt catcaatatc
     3121 aacccatttt gaccttgatt gtgcaatgtt tccagtaata tcataggtca aatttcccaa
     3181 ttttccagct gtaccaagcc taagttcaaa acctttcgca ctttgactgt ttaaattctc
     3241 aacaggtagc tcagcaccaa aagttgaggg tagagatttg gttctgcttc ccgggattcc
     3301 tgagcgtata cggtagaaaa actcaacttc accatacaat ttccggttca acaatgaaaa
     3361 atcaaccccg gcattgtaaa ttttcattgt ctcccaagtc aaattaatgt tgggtaatcc
     3421 tgtggggacg agtgcagaaa tcaattcgtt tccaattgta taagtagcat cataagcata
     3481 ccctgcaaga taagcaaagt tagcaactgc atcgtacccg gatgatccgt aact
//
LOCUS       DAOSDR010000337         3527 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrimonas sp. isolate AMR_MDS_1826
            NODE_9353_length_3527_cov_4.484735, whole genome shotgun sequence.
ACCESSION   DAOSDR010000337 DAOSDR010000000
VERSION     DAOSDR010000337.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863355
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrimonas sp. (wastewater metagenome)
  ORGANISM  Petrimonas sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Dysgonomonadaceae; Petrimonas.
REFERENCE   1  (bases 1 to 3527)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3527)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:10:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,720
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 35
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3527
                     /organism="Petrimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9353_length_3527_cov_4.484735"
                     /isolate="AMR_MDS_1826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2023866"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(1..556)
                     /locus_tag="PLI30_11460"
                     /pseudo
     CDS             complement(1..556)
                     /locus_tag="PLI30_11460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005654010.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(912..1637)
                     /locus_tag="PLI30_11465"
     CDS             complement(912..1637)
                     /locus_tag="PLI30_11465"
                     /inference="COORDINATES: protein motif:HMM:NF022441.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head GIN domain-containing protein"
                     /protein_id="HOI80206.1"
                     /translation="MKHLVFTNLLIFSFVLFANNACAQSNRITNRNYDVTAFSAMVSN
                     TVGNIEYTPSSHYSVSAEGNEEMVNNLVVTVQNSQLNLSMKKNLKRLFGNRRSGKLVI
                     RISSPSLNFIESNGVGNIELKGTLDTPQFKIVSTGVGNIAAQHFTSGNIDITSEGVGN
                     IEIKGSATSVSVVSKGVGNVKLENLKAARVRIESDGVGNVSCHATESVDINTDGIGNV
                     TYYGNPRTKNISKGGIGKVRPGD"
     gene            complement(1698..2999)
                     /locus_tag="PLI30_11470"
     CDS             complement(1698..2999)
                     /locus_tag="PLI30_11470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HOI80207.1"
                     /translation="MRTFTIIAIFCCLSTMAQAQQYTLEECINIALQNNRNIKQQELS
                     KAQRQIAYSQARNDLLPSVNASAGQNFVFGRSIGLDNTYQNTNSSQSSFGVGADITLF
                     DGLRMKHNIDAKRADLSAAEADLEKFKDEIEMSVATAFLQVLLQKELLQIASEQIQLT
                     DSNMNRRKELIKSGKMAHGEIYELEAQRAREEQNRVQAESNLKLALLDLAQIMELEDF
                     SDFNVSAPSVESILSEGVLLSTSDIFQTALLTRPEIKAAEYRLQSSEKEVLMAKSQLY
                     PSLSFGANFGTGYYNMSGRSNDPFHTQIRNNMSNSLGFSLRIPIFNKFQTKNSIRTAE
                     LAAENNRLEIDKVKIDLRKRIEQAYHNALGAQSKWKATQKSEISGQEAFRFAQEKFDN
                     GRANSYELFQAKSNLTQTLSDQAQAKYEYAFRLKILELLKK"
     gene            complement(3005..>3527)
                     /locus_tag="PLI30_11475"
     CDS             complement(3005..>3527)
                     /locus_tag="PLI30_11475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019539938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HOI80208.1"
                     /translation="NSVIQSNNFNDGTTIASVANLSDMLFVGKIDETEVGKLSVNMPM
                     EITIGAIQDKKIPAKLEYVSPKGVEESGAILFEMKAAMEMPADVFIRAGYSANADIIL
                     DRRDSVLSISESCVEFSNDSAFVYTVKSEKPQEFDRKYVKAGLSDGINIEILEGLKMN
                     DKVRGNEIIETKK"
BASE COUNT          790 a          826 c          807 g         1104 t
ORIGIN      
        1 gttaacattg cttccatacc catacccgta accgtaaccg tatccgtacc cctgtttttc
       61 gggatctatt ccgttgagca gcacgctcat gttcggcatc ttccccgatt cgtacagctc
      121 ttgaaggtcg ggaagcgcgc gtttgtcaaa cagtcccgag cgcaccacat agattgtccg
      181 gtccgcgaca cgggcgcata acaaggcgtc cacgacaacg ccatagggtg gattgtccag
      241 caggatatag tcatatcttc ctcttagctc ctcaatcatt gagtcaagcc gctcgctttt
      301 caacagttcg gccgggttgg ggggcagtgt tccggaagtg atgacatcca accggctcgt
      361 tctcttcctt cttgttcagc aggtataggt acaactcctc ctttattttc tgctgccgct
      421 ccacgctgat cacgtatttt tgttgtgtgg gcacgacgaa ggctatcttt cccctgtttt
      481 cccgctcctt gttcttcatg ttttccgtct gtatctttag gccggcgatc aggttgtcca
      541 cggctttgga tcttgcctgt aatttgagtt gatcgatttt gtgctgctca gtggcgcaac
      601 ctattgtttt cgccaccttg tatctgccgc gacacttgct aattatctga atgctaacga
      661 tcccagagcg gtttttcttt tctcggatga acatgttgaa aaaatcggga cacccaaaat
      721 cagtaaatta ttttcatatt ccgttgatta acagtgaaat aatttgggtg tcccgaaaaa
      781 gtgcggaaga caggaaaaat ggtggagtga cggatcgtga tagtggcata caaataaaaa
      841 cgggagcttt caaacaagcg cccgtttttt tgattagagt ttaatctaaa agtagagtgt
      901 tatattgttc gttaatctcc tggccttact ttcccgattc ctcccttgct gatgtttttg
      961 gtgcgtgggt tgccgtagta agtgacattc ccgattccgt ctgtattgat gtccaccgat
     1021 tctgtagcgt ggcacgaaac atttcccaca ccatccgatt cgattcgcac acgagcggct
     1081 ttcaggtttt caagctttac atttcccacg ccttttgata cgacagatac cgaagtggcc
     1141 gatcccttta tttctatatt ccctacccct tcagaagtaa tgtcgatatt gccggaggta
     1201 aaatgttgag cagcgatgtt tcccacgccc gtggagacaa tcttgaattg aggtgtatcc
     1261 agggtccctt tcaattcaat gtttcccacg ccgtttgatt caataaaatt gagggagggt
     1321 gaacttatcc tgatgaccag ttttccggat cgccggttgc cgaatagcct ttttaggttt
     1381 ttcttcatcg ataggttgag ttggctgttt tgtacggtta ccaccaagtt gttaaccatc
     1441 tcttcgttgc cttcggcgct tacactgtaa tgggaagacg gagtgtattc tatgttgccg
     1501 accgtgtttg ataccatggc ggaaaatgcc gtcacatcgt aatttctgtt cgtgatacgg
     1561 ttgctttgag cacatgcgtt gttcgcaaag agcacaaagg agaaaatcaa tagatttgta
     1621 aatacgagat gtttcataat gggagtcatt aatgtttaca tataagacgt tcggttttca
     1681 gtttcggtta caagaaatca tttttttaac aattccagta tctttaggcg aaaagcatat
     1741 tcgtattttg cctgcgcctg atcggataat gtttgtgtga ggttgctttt cgcttgaaaa
     1801 agttcgtagg agttagcgcg tccgttatca aatttctctt gtgcgaaacg gaacgcttcc
     1861 tgtcccgata tttctgattt ttgcgttgct ttccatttgc tttgagcacc taaggcgttg
     1921 tggtacgcct gctcaatgcg tttgcggaga tcgattttca ctttatctat ctccaaccgg
     1981 ttgttttctg cggcaagttc tgccgtgcgg atactgtttt ttgtctggaa tttattgaag
     2041 atagggattc gcaggctgaa cccgagcgaa ttgctcatgt tgttccggat ttgagtgtga
     2101 aacggatcgt tcgagcgtcc gctcatgttg tagtatccgg ttccgaagtt agcgccgaaa
     2161 gagagggagg ggtagagttg cgacttagcc atcaatactt ctttttcgct actttgaagc
     2221 cggtattcag ctgctttgat ttcgggacgg gtaagcaacg cagtttgaaa aatatcggag
     2281 gttgagagta gtactccttc actgagaatt gactccaccg atggtgccga gacattaaaa
     2341 tctgaaaaat cctccagctc cataatctgg gccagatcaa gcaacgccag cttgaggttg
     2401 ctttctgcct ggaccctgtt ctgttcttct cgggcacgct gggcttccag ttcataaatc
     2461 tcgccatgag ccatcttgcc actcttgatc agttccttcc ggcgattcat attggagtcc
     2521 gtcaattgaa tttgttcgct ggcaatctgc aacagctcct tttgcagcag cacctgtaga
     2581 aaagcggtcg ccacgctcat ttctatttcg tctttaaact tctccagatc ggcttcggca
     2641 gcacttaaat cggcgcgttt agcatcgata ttgtgtttca tccgcaatcc gtcaaacaac
     2701 gtaatgtccg cgcccactcc aaacgagctc tgagatgagt tggtgttttg ataggtattg
     2761 tcaagtccta tagatcggcc aaaaacaaag ttttgtcctg cggatgcgtt tacactgggt
     2821 aacagatcgt ttcttgcttg tgagtatgcg atttggcgct gtgccttgct cagctcttgc
     2881 tgcttgatgt ttctgttgtt ttgcaaggca atattgatgc actcttcaag cgtatattgc
     2941 tgcgcttggg ccatcgttga caaacaacaa aaaatagcga taatggtaaa tgtcctcata
     3001 cttcttattt cttcgtttca atgatctcat tgccacgtac cttatcattc attttcaacc
     3061 cttcaaggat ttcaatattg atcccatcag aaaggcccgc ttttacgtac ttgcggtcaa
     3121 attcctgtgg cttctcgctt tttacggtat acacaaacgc cgaatcattg ctgaactcaa
     3181 cacagctctc ggaaatggaa agcacactgt cgcgacggtc gaggatgata tcggcgttgg
     3241 cgctgtatcc ggcacggata aagacatccg ccggcatctc catggcggct ttcatctcaa
     3301 aaaggatagc tccgctctct tccacgcctt tgggagaaac atattccagt tttgccggaa
     3361 ttttcttgtc ctgaatggct ccgatggtga tttccatagg catattcaca gacagttttc
     3421 ccacctcggt ttcgtcaatc tttcctacga aaagcatatc gctcaggttg gcaacggatg
     3481 cgatggtagt gccgtcgttg aagttgttgg actgtataac cgagttt
//
LOCUS       DAOSDR010000338         3506 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrimonas sp. isolate AMR_MDS_1826
            NODE_9423_length_3506_cov_3.811649, whole genome shotgun sequence.
ACCESSION   DAOSDR010000338 DAOSDR010000000
VERSION     DAOSDR010000338.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863355
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrimonas sp. (wastewater metagenome)
  ORGANISM  Petrimonas sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Dysgonomonadaceae; Petrimonas.
REFERENCE   1  (bases 1 to 3506)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3506)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:10:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,720
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 35
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3506
                     /organism="Petrimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9423_length_3506_cov_3.811649"
                     /isolate="AMR_MDS_1826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2023866"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(125..547)
                     /gene="tsaE"
                     /locus_tag="PLI30_11480"
     CDS             complement(125..547)
                     /gene="tsaE"
                     /locus_tag="PLI30_11480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006847848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HOI80209.1"
                     /translation="MQLKISDISEIHKAAGQFIAGMGNDKVFAFYGAMGAGKTTFIKA
                     VCEGLGVQETITSPTFAIINEYRDNEGNSIYHFDFYRIHKLEEAFDFGYEDYFYSGNL
                     CFIEWPELVEPLLPGNTVKVYITGTDAGTRTIETKKPD"
     gene            complement(571..2115)
                     /locus_tag="PLI30_11485"
     CDS             complement(571..2115)
                     /locus_tag="PLI30_11485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006841647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PglZ domain-containing protein"
                     /protein_id="HOI80210.1"
                     /translation="MKKAHILWADDEIDLLRPYVLFLEEKGYGVDTVNSGRDAIEMCR
                     SSVYDIIFLDEHMPGLTGLETLSEIAASHPELPVVMITKSEDEGIMDKAIGKKIADYL
                     IKPVNPNQILMTIKKKLEKNEIISETNTIHYREEFGRLSSEMNNCRSAEGWTALYKKL
                     VSWEVELALLQHPLQELLTVQKTEANAGFGKFVKRNYENWLLNAGSGPLLSNEVFQQR
                     VFPVLDKGEKLFFILIDNFRFDQWLVIKDLVSDYFTYTEDTYFSILPTATQYARNAIF
                     SGLMPLQLSKKFAGLWVDEVEDEGKNLSEELLVRSQLERFRRKERFSYNKINSNTEGE
                     RLVQNFTGLEHNELNVVVFNFIDMLSHARTESKMIRELAPDEPAYRSLTRSWFRHSPL
                     FGLLRKISEKKYRVMLTTDHGTIRVRHAQKVEGEKNTNTSLRYKVGRNLSYDPKKVFS
                     VTHPEKVGLPSPNISTRYIFALGDDFFVYPNQFNHYVSYYENTFQHGGISMEEMIVPL
                     VTLQAR"
     gene            2306..2944
                     /locus_tag="PLI30_11490"
     CDS             2306..2944
                     /locus_tag="PLI30_11490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006798605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1080 domain-containing protein"
                     /protein_id="HOI80211.1"
                     /translation="MKKTNLLVFAVLIVLASGCAQDNGKIFNGKDLSNWNFVVENGAV
                     PGEQVYTVKDGQIAIKGEPLGYMYTKEKYANYTLELEYRWAEEASNSGIFVLIEDPKN
                     PFATGIEVQLAAGKAGDFVLLAGSDMKEYTLPEGVTERPKFPVIQKKQPSSENPAGEW
                     NKVKITVQDGVVDVYVNDVHQNTGTGLVKEGNIGLQSEGKEILFRNLVLTKM"
     gene            2976..3455
                     /locus_tag="PLI30_11495"
                     /pseudo
     CDS             2976..3455
                     /locus_tag="PLI30_11495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875102.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
BASE COUNT          986 a          802 c          728 g          990 t
ORIGIN      
        1 tattcacctg cgaagagaca aaaagtgcgg acagaaacat acaaacggat atagctattt
       61 ttctcatgta tatggtaatt ctttttactg aactgactat tggatcattt aaatctccat
      121 tcgtttaatc gggctttttt gtttcgattg tccgcgtgcc ggcatcagtt ccggtaatat
      181 aaacttttac ggtattgccg gggagaagcg gctctaccaa ttccggccat tcgataaaac
      241 aaagatttcc gctgtaaaaa taatcttcgt aaccgaaatc gaaagcttct tcaagtttat
      301 ggatgcggta aaaatcgaaa tgataaatgg aatttccttc gttatccctg tactcattga
      361 taatggcgaa tgtgggactg gtaatcgttt cctgcacgcc caatccttca caaacggctt
      421 tgataaaagt ggttttaccg gcacccattg ctccatagaa agcaaaaacc ttatcgttcc
      481 ccattccggc aataaattgt ccagctgcct tatgtatctc ggatatatca cttattttca
      541 attgcatact cttttccttt tattttttat ttacctggct tgcaacgtta ccaacggaac
      601 aatcatctct tccatggaaa ttccaccgtg ctgaaacgta ttttcgtaat acgaaacgta
      661 gtggttgaat tgattcggat acacgaaaaa atcgtcgcca agagcaaaaa tataccttgt
      721 actgatgttt ggtgagggta agcctacttt ttcgggatgg gtaacgctaa acactttttt
      781 gggatcgtat gacaggttcc gacccacttt gtaacgcaaa ctggtattgg tatttttttc
      841 accttccact ttttgcgcat gacgcacacg gatagtcccg tgatcagtgg taagcattac
      901 ccggtatttt ttttccgata tctttctcaa caacccaaac aaaggcgaat ggcgaaacca
      961 cgaacgcgtt agcgaccgat aggccggctc gtcgggtgcc aactcacgga tcattttcga
     1021 ttcggtccgt gcatgagaaa gcatatctat aaaattaaaa actaccacat tcagttcgtt
     1081 gtgttccagt ccggtaaaat tttgcacaag cctttcccct tcagtatttg aattgatctt
     1141 gttgtatgaa aaccgctcct ttcgcctgaa tctttccagt tgtgaacgaa ccagcagttc
     1201 ttccgacaaa ttcttaccct cgtcctcaac ttcatccacc cacagtccgg caaacttttt
     1261 cgacagctgc aacggcatta accccgaaaa aatggcatta cgggcgtatt gggttgcggt
     1321 gggcaaaata ctgaaataag tatcttcggt atatgtaaaa taatccgaca ccaggtcttt
     1381 gatcaccagc cattgatcga accggaagtt atcgatcagg ataaaaaaga gtttttcacc
     1441 cttatccaac accggaaaaa cccgttgttg aaaaacttca ttacttagca atgggcctga
     1501 tccggcattc aacagccagt tttcgtaatt tcgtttcacg aactttccaa atccggcgtt
     1561 tgcttccgtt ttttgcacgg tcagcagttc ctgcagcgga tgttgcaaaa gagctaattc
     1621 aacctcccag gaaacaagtt ttttatacaa tgcggtccaa ccttctgccg aacggcaatt
     1681 attcatttca ctgcttagcc gaccaaactc ctcccggtaa tgaatagtgt ttgtttcact
     1741 gattatttcg tttttctcca gttttttttt gatggtcatc aggatctgat tgggattgac
     1801 cggctttatc aggtaatcag caattttctt gccgatggcc ttgtccataa taccttcatc
     1861 ctcacttttc gtgatcatca ctaccggcag ttccggatga gaagcagcaa tctcggagag
     1921 tgtctccaag cccgtcaatc cgggcatgtg ttcatccagg aaaatgatat cgtaaaccga
     1981 gctccggcac atctcaatgg catcacgacc gctattcacg gtatccaccc cgtacccctt
     2041 ttcttccaaa aaaaggacat aaggtctgag taggtcaatt tcgtcgtccg cccataggat
     2101 gtgcgctttt ttcatttttc ctttaatgat ttacctgcag aaaattgagc aaaaatatgc
     2161 atttattgcc tatctcacaa tcattttttg cttttttagg actatatccc gtatcgtccg
     2221 acttttatta cccaaacctg tctatattct cagcaatttt acttaacttt gttaacaaga
     2281 tatcaaccat ctatttatac aacaaatgaa aaagacaaat ctacttgtat ttgcagtatt
     2341 aattgttctt gcttcaggct gtgcgcaaga caacggaaaa attttcaacg gaaaagattt
     2401 atcgaactgg aactttgtcg tcgagaacgg tgctgttccg ggagaacaag tgtacacggt
     2461 aaaagacgga cagatcgcta ttaaagggga gccattgggt tatatgtaca cgaaagaaaa
     2521 atatgccaac tacaccctgg aactggaata ccgttgggct gaagaagcca gcaacagtgg
     2581 gatttttgtg cttatcgagg atcccaaaaa tccatttgcc acaggcattg aagtgcaact
     2641 ggcagcaggc aaggccggcg actttgtatt gcttgccgga tccgacatga aggagtacac
     2701 gctcccggaa ggtgtgacgg agcgtccgaa attcccggtc atccagaaaa aacaaccttc
     2761 gagcgaaaat cccgccggcg aatggaataa agtaaaaatt accgttcagg atggagtggt
     2821 agacgtttat gtgaacgacg tgcatcaaaa cacgggaacc ggactggtta aagaaggaaa
     2881 catcgggttg cagagtgaag gaaaagagat tctgttccgg aacctggtcc tgaccaagat
     2941 gtgaagtaat caaaatcact atttatgtta atcacatgtt aatcacagaa aaagcacaac
     3001 aatttatcga tcaggaggac agattcgggg cgcacaatta tcacccgctg cccgtagtac
     3061 tgtcaagggg gaaaggtgta tatgtctggg atgttgatgg caaaaaatac ttcgacttcc
     3121 tttccgccta ttctgccgtg aatcagggac attgtcaccc gaagatcgtg agggcactga
     3181 ccgaacaagc ggaaacattg tgccttactt cgcgcgcatt ttacaacgac tgcctgggcc
     3241 cgtacgaaga gtttgtccac cgttacttcg gttacgacaa gatgttgccc atgaattcgg
     3301 gagcagaagc cgttgaaacc ggattgaagc tcgcccgaaa atggggttac ctgaaaaaag
     3361 gaatccccga aaatgaggcc attattgtcg cttgcgaagg aaattttcac ggacgtacca
     3421 ttacaatcgt ttccctttcc accgatgggg aagcccgtga aaatttcctt cgcaagcgac
     3481 aataatggcc tcattttcgg ggaaat
//
LOCUS       DAOSDR010000339         3495 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrimonas sp. isolate AMR_MDS_1826
            NODE_9470_length_3495_cov_4.311047, whole genome shotgun sequence.
ACCESSION   DAOSDR010000339 DAOSDR010000000
VERSION     DAOSDR010000339.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863355
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrimonas sp. (wastewater metagenome)
  ORGANISM  Petrimonas sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Dysgonomonadaceae; Petrimonas.
REFERENCE   1  (bases 1 to 3495)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3495)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:10:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,720
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 35
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3495
                     /organism="Petrimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9470_length_3495_cov_4.311047"
                     /isolate="AMR_MDS_1826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2023866"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1235
                     /locus_tag="PLI30_11500"
     CDS             <1..1235
                     /locus_tag="PLI30_11500"
                     /inference="COORDINATES: protein motif:HMM:NF019592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HOI80212.1"
                     /translation="RLLSFVSGELMNGGYESSNDLVSFQDNNRRQRLTDAKNAAKDII
                     DGKFGHYALSGATHEPPLNMTNEELMAYAENYSNIFLQKGAWNDEVIFGIQYLNRQGN
                     RAVNNLYWGPNGYNCWGNNEPTEHIVRQFEMEDGTSFKWDKYNPGQINIREFTKTQLD
                     ANPELNPYVGREPRFYANILFDGAPWRDRSSTNNKIQIASQVDAPGASIMGAKSVAEK
                     MQAIATLEPVITGGNDSRQAANQAWNGTKTGYYLRKMLDISIDGEIDNNENTWLEMRF
                     AEVLLDYAEACIELGEIEEGITTINKIRNRAGLPGRSITVSQEQAREWYRHERLIEMF
                     AEGDRWYSIRKWMICDKVIADFHPMYIYHFNDGVSMYIYNTTALADTRKWDNKNYWLP
                     ISRTEINKAPQLKQNPGY"
     gene            1253..2575
                     /locus_tag="PLI30_11505"
     CDS             1253..2575
                     /locus_tag="PLI30_11505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019538107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOI80213.1"
                     /translation="MNYSRRKFIKTAGLATAGTIISSRVALGSNSIWTSNSNTLKVGL
                     IGCGGRGTGAAGEALNADPNVILTAMADAFEDQLQTSFNTLKEKYGNKVQVPESNKFA
                     GLDAFQKLIDSDVDVVLLAAPPCFRPQHLEAAVNANKHVFCEKPFAVDAPGLRRVMDA
                     AKKAKAKNLSLVSGFCWRYHQPKRDTFGRVLNGQIGEILGGEATYNTGELWFKERQRG
                     WSDMEYKLRNWLYYNWLSGDHLIEQAIHSIDMFSWAMGDKAPLKISGTGGRQKRTDPK
                     FGNVYDHFGLTYEYDNGVKIYFFCRQQAETTPSYAVELIGKDGRCYVDCRTGEHKITG
                     KNAWNLADATKFTDAEAYKQTKVRGMYQVEHDELFASIRANKPMNDGEWMTRSNLLAL
                     AGRMAAYSGQTITFDQALNSSEVLFPGDISRDTKYDLPIAIPGVTAFK"
     gene            2582..>3495
                     /locus_tag="PLI30_11510"
     CDS             2582..>3495
                     /locus_tag="PLI30_11510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019538106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HOI80214.1"
                     /translation="MNKRDFIKSAAVMAGVSAITPFANVSGKNKTPSAAAQQKSKVSL
                     KKALVFGMIQDKGSLTDKFKIARDAGFDGVELNSPTDLSLTDLLQAKRNAGIELPSVI
                     NKDHWSKPLSDPDQQIRQQCIESVIKSMHEVKELGGDTVLVVPGVVNEKVSYEQAYIT
                     SQSAIRELIPHAEKTGVQIALENVWNNFILSPVEAKRFIDELNHPLVGWYFDIGNVLR
                     YGWPEHWIHTLKSRIMRLHIKEFSRENMNSKGLREGFKVELLEGDNNWPVVMKAVRDT
                     GHKGGWLTAEVPGGDLTHLKKISALMDK"
BASE COUNT         1112 a          758 c          858 g          767 t
ORIGIN      
        1 caagactttt aagttttgtt tcaggagaat tgatgaatgg cggatatgaa tcttcgaacg
       61 atttagtttc ctttcaagac aataacagaa gacaacgact tacggatgcg aaaaatgcgg
      121 ccaaagatat tattgacgga aaatttggac attatgccct ttcaggtgct acccatgagc
      181 cccctttaaa tatgacgaat gaagaactga tggcttacgc tgaaaattat agcaatattt
      241 ttctacaaaa aggagcttgg aatgacgagg ttatttttgg aatacaatac cttaacaggc
      301 agggaaacag agccgtcaac aacctgtatt ggggaccaaa cggatacaat tgttggggga
      361 ataacgaacc cacagaacat attgttagac aatttgaaat ggaagatgga acttcgttca
      421 aatgggataa gtacaatccg ggacaaataa acattcggga atttacaaaa acgcaattgg
      481 atgcaaatcc ggaactaaac ccttatgttg gcagagaacc tcgtttctat gcaaatattc
      541 ttttcgatgg agctccttgg cgcgatagat catcaacaaa taacaagatt caaatagcct
      601 cacaagtaga tgctcccgga gcaagtatta tgggtgcgaa aagcgtggcc gaaaaaatgc
      661 aagccatagc aacgcttgaa cctgtcatta cgggaggaaa cgactccaga caagccgcta
      721 accaagcatg gaacggaaca aaaacaggtt attacctaag aaaaatgctg gacattagta
      781 ttgatggaga gatagataat aatgaaaata cttggctcga aatgcgtttt gcagaagtat
      841 tgttggatta tgccgaagcc tgtatcgaat tgggagaaat agaagaaggc attacaacca
      901 tcaacaaaat cagaaaccgt gccgggctac caggacgttc aattaccgtt agtcaagagc
      961 aggcaagaga atggtataga cacgaaagat taattgaaat gttcgccgaa ggagatagat
     1021 ggtacagtat acgaaaatgg atgatttgcg ataaagtaat tgcagatttc catccaatgt
     1081 atatttacca ctttaatgac ggagtatcaa tgtacatata taatacaaca gctctggcag
     1141 atacaagaaa atgggataat aagaattatt ggctgcctat ttcaaggaca gaaataaaca
     1201 aggctcccca gttaaaacaa aatccgggtt actaaatcaa aataaaacaa caatgaacta
     1261 ttcaagaaga aaatttatca aaactgcagg actggctact gccggaacaa ttatctcttc
     1321 cagggtcgcc ttggggtcta actccatctg gacgtccaac agcaacacgc tgaaagtggg
     1381 gctgatcggt tgtggtggcc gcgggacagg tgccgcagga gaagcattga atgccgatcc
     1441 gaacgtcatc ttaaccgcaa tggcggatgc ttttgaagat cagctgcaaa catcattcaa
     1501 cacccttaaa gaaaagtacg ggaataaagt gcaggtgccg gaaagcaata aattcgccgg
     1561 tctcgatgcc ttccaaaaat tgatcgactc cgatgtggat gttgtcctgc ttgccgcgcc
     1621 cccctgtttc aggcctcaac acctggaagc agccgttaac gccaacaagc atgtcttctg
     1681 tgagaaaccc tttgccgtgg acgctcccgg tttacgaagg gtgatggatg ccgcaaagaa
     1741 ggccaaggca aaaaacctgt ccctggtttc cggattctgc tggaggtacc atcaacccaa
     1801 aagagatacc ttcggaaggg ttctgaacgg tcagatcgga gagatcctgg gtggggaggc
     1861 aacttacaac accggtgaac tgtggttcaa ggaaagacaa cgcggctggt cagacatgga
     1921 gtacaaactc cgtaactggc tttattacaa ctggctttcg ggagaccacc ttattgaaca
     1981 ggccatccac agtatcgata tgttctcctg ggcaatgggg gacaaagctc cgctgaaaat
     2041 cagcggtacc ggcggaaggc aaaagagaac cgatccgaag ttcggaaatg tatacgacca
     2101 tttcggactg acctacgaat atgataacgg cgtgaaaata tatttcttct gccgtcaaca
     2161 agcggaaaca accccttcct atgcggttga attaattgga aaggacggta gatgctacgt
     2221 agactgtcga accggcgaac ataaaattac cggaaaaaac gcgtggaacc tggctgacgc
     2281 gaccaaattc acagatgcgg aagcttacaa gcagacaaag gtaagaggca tgtaccaggt
     2341 ggagcacgac gaattgtttg cttccatacg ggcaaacaag ccgatgaacg acggcgagtg
     2401 gatgacacgc tccaacctgc tcgctctggc cggccgcatg gctgcctact cgggacagac
     2461 catcaccttt gaccaggcgc tgaactcatc ggaggtattg ttcccgggag atatttcccg
     2521 ggatacaaag tacgaccttc ccattgcgat accgggggtt acagcattta aataaaccaa
     2581 catgaacaaa agagatttta tcaaatcggc agcggtgatg gcgggagttt ctgccatcac
     2641 cccgtttgca aacgtatcgg gcaaaaataa aacaccatct gcggcggcac agcaaaaatc
     2701 caaggtgtcg ttaaaaaaag cgttggtctt cgggatgatc caggacaagg gttcactcac
     2761 ggacaagttc aagattgccc gggatgcggg ttttgacgga gttgagctca atagtccgac
     2821 cgacctttcc ttaaccgatc tccttcaggc aaaacggaat gccggcatag aactgcccag
     2881 cgtgatcaat aaggaccatt ggtcaaaacc attatcggat ccggatcaac aaatcaggca
     2941 gcaatgcatt gaatcggtga tcaagtcaat gcatgaagtg aaagaactgg gtggagacac
     3001 cgtattggtc gttccaggcg tagtgaatga aaaggtatcc tatgaacaag cgtacataac
     3061 ctcgcaaagc gcgatcagag aactgattcc ccatgctgaa aaaacgggtg tgcaaattgc
     3121 cctggaaaac gtttggaaca actttatcct cagccccgta gaagcaaagc gatttatcga
     3181 tgaactcaac cacccgctgg tcggctggta tttcgatatc ggaaatgtgc tccggtacgg
     3241 atggccggaa cactggatcc acaccttgaa gtcccgtatc atgcggctgc acatcaagga
     3301 gttcagccgt gagaacatga actcgaaagg attaagggag ggctttaagg tagaactcct
     3361 tgagggagac aacaactggc cggtggtcat gaaagccgta cgggacactg gccataaagg
     3421 aggttggctg accgctgaag ttcccggtgg agatttaacg catttaaaga aaatctccgc
     3481 actgatggac aagat
//
LOCUS       DAOSDR010000340         3490 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrimonas sp. isolate AMR_MDS_1826
            NODE_9494_length_3490_cov_4.039010, whole genome shotgun sequence.
ACCESSION   DAOSDR010000340 DAOSDR010000000
VERSION     DAOSDR010000340.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863355
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrimonas sp. (wastewater metagenome)
  ORGANISM  Petrimonas sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Dysgonomonadaceae; Petrimonas.
REFERENCE   1  (bases 1 to 3490)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3490)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:10:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,720
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 35
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3490
                     /organism="Petrimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9494_length_3490_cov_4.039010"
                     /isolate="AMR_MDS_1826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2023866"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..825
                     /locus_tag="PLI30_11515"
     CDS             <1..825
                     /locus_tag="PLI30_11515"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HOI80215.1"
                     /translation="GILLMKGNLQQHEILENELQRNYDNVQTYVQNGVANDVDLSTVK
                     VEQLKAGQQRIQLESTLDAYIRMLSVLTGEPLEKNTTFVKPNPEVELISPIINRPELK
                     MFTAQEEAAESQKSLLKAKNMPKLSAFAQGGYGKPGLNMFDTKFSPYFLGGIRLSWNI
                     GNLYTYGNDKKKIDLQKQSVNTQKETFLYNLKVQIPQQQLEIEKYKKTMLDDDEIIRH
                     QTLIREAAEVKVENGTLTVSDLMKEVNSEEAVKQAKTLHEVQYLMSIYSLKHTINQ"
     gene            1051..1947
                     /locus_tag="PLI30_11520"
     CDS             1051..1947
                     /locus_tag="PLI30_11520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005788227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HOI80216.1"
                     /translation="MKLFRLLTTLSALFLLFSCSNGKGDYDATGSFEATEIIVSSQAN
                     GRILELGLNEGDQLEQGQEVGLIDSTQLYLQKMSLLSNAKGVRAQQPDIKAQTAALQE
                     QIKSLEREKARLGRLVTANAANQKQLDDIDSQLEVVRRQLSALTSTLQKSSDNISAQS
                     STLDIQVAQLEDQLDKTRIVSPISGTVLNRYAEAGELATIGTPLFKIADTGILFLRAY
                     VTNDQLAQIKLNGEVTVRVDDGHGGMKSYKGRINWISDKSEFTPKTIQTKNERANLVY
                     ALKIAVPNDGFLKIGMYGEVKL"
     gene            1960..2865
                     /locus_tag="PLI30_11525"
     CDS             1960..2865
                     /locus_tag="PLI30_11525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018108696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOI80217.1"
                     /translation="MIEVKDLHKSYGRQGKVNALNSISFTVDDGEIFGVIGPDGAGKS
                     SLFRILASLILPDSGSAIMNGHDVVKDYRKIRQIIGYMPGRFSLYQDLSVEENLVFFA
                     TLFNTTVEENYHLIKDIYQQIEPFKKRRAGALSGGMKQKLALSCALIHKPEILILDEP
                     TTGVDPVSRKEFWDMLGRLKEQGITILVSTAYMDEASRCDRIALIREGEFIANDTPQG
                     IIDDYPEILWAVEGTGMSALLGELRAHDKVKSSFAFGDKIHITVEKSLQTEELKRYLA
                     NKKFNDVRIAPIAPTVEDCFMALTS"
     gene            2869..>3490
                     /locus_tag="PLI30_11530"
     CDS             2869..>3490
                     /locus_tag="PLI30_11530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009317277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOI80218.1"
                     /translation="MSKKVIEVFNLTKKFGNFTAVDRISFDVKEGEIFGFLGANGAGK
                     TTAMQMLCGISMPTAGEGTVAGYDIIKENERIKRNIGYMSQKFSLYEDLKVWENIRLF
                     GGIYGLSDKTIAEKTNELLKQLDFEQERNTLVKYLPLGWKQKLAFSVAIFHEPRIVFL
                     DEPTGGVDPAMRRQFWEMIYDAADRGITVFVTTHYMDEAEYCNRVSI"
BASE COUNT         1054 a          777 c          890 g          769 t
ORIGIN      
        1 ggcattctgc tgatgaaggg aaacctgcag caacatgaga tcctggaaaa tgaactgcag
       61 cgcaactacg ataacgtgca aacctatgtg caaaacggcg tagctaacga cgttgacttg
      121 agcacagtaa aggtagaaca gctgaaagca ggacaacagc ggattcagtt ggaatctacg
      181 ttagatgctt atatacggat gttatccgtg ctcaccggag aaccgctcga aaagaatacg
      241 acatttgtaa aaccaaatcc cgaagtagaa ttgatatctc ccatcatcaa tcgtcccgag
      301 ttaaagatgt tcaccgcaca ggaggaggcc gctgaatcgc aaaaatcgct cctgaaagcc
      361 aaaaacatgc caaagttgtc cgcctttgca cagggaggtt acggaaaacc gggactcaac
      421 atgttcgaca ccaaattttc cccctacttt ttgggaggga tccgcctctc atggaacatt
      481 ggcaatctat atacatacgg taacgacaag aagaagatcg atctgcaaaa acagtcggtg
      541 aacacgcaaa aggagacttt cctgtataat ctgaaagtac agataccgca gcagcagttg
      601 gagattgaaa aatataaaaa gaccatgctg gacgatgacg agatcatacg ccatcaaacg
      661 ctgatccgtg aagctgccga ggtgaaagtg gaaaacggca ccctgacggt atccgacctg
      721 atgaaggagg tcaattcgga agaggcggtc aagcaggcga aaacattgca tgaagttcaa
      781 tacctcatgt cgatctattc actgaaacat acaataaatc aataaaatgg aggctgtcta
      841 tatgtgacta aatcatcgtc tgacgatatt ttgcgaccca ttctcgctcg tgtcaactaa
      901 aagagtagaa actcgcttag ctcaaacaac tactcttttt tacgtttatc gcgagctatg
      961 ggtatcccgt aaatagctaa aaggtctctg ttttagtcgc ataagctatc tgaggatatc
     1021 tactaaaatt caataaaaca acattaaacg atgaaactat ttagattact aacaacatta
     1081 tccgccttgt tcctgctctt ttcatgcagc aacggcaaag gcgactatga cgctaccgga
     1141 tcgtttgaag caacggaaat tatcgtctca tcacaggcga atggacgcat tttagagctg
     1201 gggctgaatg agggagatca gctggaacaa gggcaagaag tgggactcat cgacagcacc
     1261 cagctctatc tgcaaaaaat gtccttgctc tccaacgcga aaggtgtccg tgcccagcag
     1321 cccgatataa aagcgcagac ggcagcttta caggagcaaa tcaaatcatt ggagcgggaa
     1381 aaagcacggt tagggagatt ggttaccgcc aatgccgcca atcagaaaca gctggacgac
     1441 attgactccc aattggaggt tgtgaggagg cagctatcgg cgctgacatc cacattacaa
     1501 aaaagcagtg ataatatttc cgcacaaagc tccacgctcg atattcaggt cgcacaactg
     1561 gaagatcaac tggacaagac ccgtatcgtc agccctattt cggggaccgt actcaacaga
     1621 tatgcagagg caggtgagct ggctaccata ggtactcccc tattcaaaat tgccgatacc
     1681 ggaatcctat tcctgagggc ctacgtgaca aatgatcagc tggcacaaat caaactgaac
     1741 ggtgaggtga cggtacgggt tgacgacggg cacggcggaa tgaagtcata caagggccgg
     1801 atcaactgga tatccgataa atcggagttt actcccaaga ccattcagac caagaatgaa
     1861 cgggctaact tagtgtatgc gctgaaaatt gccgtcccga acgatggttt cctgaagatc
     1921 ggcatgtatg gagaggttaa gctttaattt gcaacaaaaa tgattgaagt aaaggattta
     1981 cataaaagtt acggcaggca gggtaaggtg aatgccctga acagcatctc tttcacagta
     2041 gatgacgggg agatattcgg tgtgattggt ccggacggtg ccgggaaatc gtcgctcttc
     2101 cggatcctgg catcactgat cctgcccgat agcggttcgg caataatgaa tgggcacgac
     2161 gtggtgaagg attaccggaa gatacgacag attatcggtt acatgccggg ccggttctca
     2221 ctctatcagg atctgagcgt ggaggagaac ctagtatttt tcgccacgct cttcaataca
     2281 accgtagaag agaactacca cctgatcaaa gatatttacc aacaaatcga gccctttaaa
     2341 aaacggcgtg ccggagcgct ctcgggcgga atgaaacaga agctggccct tagctgcgca
     2401 ctgatccata aaccggagat cctgatcctg gacgagccga ccacaggggt agacccggtc
     2461 tcaagaaagg agttctggga tatgctgggg cggctgaagg agcaggggat taccattctg
     2521 gtgtcgacag catatatgga tgaagcgagc cgttgcgaca ggatagcgct gatccgtgaa
     2581 ggggaattta ttgcaaacga cactccacaa ggaatcattg atgattatcc ggagatactc
     2641 tgggcagtgg aaggaaccgg catgtcggca ctgctcggtg agctgcgcgc tcacgataag
     2701 gtaaagagca gtttcgcgtt tggcgacaag attcacatca ccgtagaaaa aagcctgcag
     2761 acggaagagt tgaagcgata cctggcgaac aaaaaattca acgacgtacg tatcgctccc
     2821 atagcaccca ctgtggagga ttgttttatg gcattgacaa gttaggacat gagcaaaaaa
     2881 gttatcgagg tattcaacct gacaaaaaag tttggcaatt tcacggccgt ggaccgcatc
     2941 tcttttgatg tgaaggaagg tgagattttt ggttttctgg gtgccaacgg cgcgggtaaa
     3001 accactgcca tgcaaatgct ttgcggtatc agcatgccga cggcaggaga aggaaccgtg
     3061 gctggttatg atatcattaa agaaaatgaa cggattaaac ggaatatagg atacatgagc
     3121 cagaaattct ccctgtacga agacctgaaa gtttgggaga atatccgcct gttcgggggt
     3181 atctacgggt tatctgataa aacaattgcg gaaaagacga acgagttgtt gaagcaattg
     3241 gattttgaac aggagcgcaa caccctggtg aaatacctcc cgttgggatg gaaacaaaaa
     3301 ctagcttttt ccgtggccat ctttcatgag cccaggattg tctttctgga tgaacctacg
     3361 ggcggagttg acccggccat gcgtcggcag ttctgggaga tgatctacga tgcggccgac
     3421 cgggggatca ccgtttttgt aaccacgcac tacatggacg aagctgaata ctgcaacagg
     3481 gtatcgatca
//
LOCUS       DAOSDR010000341         3490 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrimonas sp. isolate AMR_MDS_1826
            NODE_9495_length_3490_cov_3.619214, whole genome shotgun sequence.
ACCESSION   DAOSDR010000341 DAOSDR010000000
VERSION     DAOSDR010000341.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863355
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrimonas sp. (wastewater metagenome)
  ORGANISM  Petrimonas sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Dysgonomonadaceae; Petrimonas.
REFERENCE   1  (bases 1 to 3490)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3490)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:10:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,720
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 35
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3490
                     /organism="Petrimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9495_length_3490_cov_3.619214"
                     /isolate="AMR_MDS_1826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2023866"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..904
                     /locus_tag="PLI30_11535"
     CDS             <1..904
                     /locus_tag="PLI30_11535"
                     /inference="COORDINATES: protein motif:HMM:NF016013.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF389 domain-containing protein"
                     /protein_id="HOI80219.1"
                     /translation="SSKLKGNVIPGVAIATALMPPICTAGYGLATLQFNFFFGALYLF
                     TINTVFIAFAALVVCQYLKFPIGSIVDPDRKKRVNRALTAVILITLVPSVYFGYQLVQ
                     NENFSQHAEYFISQVSVVEGNYLQKSNINPSNKNVDLVYNGYGLSPKHIDSIKLVAFF
                     NKIDTAKLSISQGFEASFVQENTRRYQSETEVLNNRLNATTFALKQAENRIDSIRNIP
                     ALGEKLLSEIQPLYPQISGCSYTETFSYFMRKDSILRSNRTPLVIFTVSRNTLRTADK
                     NKIGEWLKSRLEKPEVLTYYDEIR"
     gene            1006..2463
                     /locus_tag="PLI30_11540"
     CDS             1006..2463
                     /locus_tag="PLI30_11540"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOI80220.1"
                     /translation="MARLLILGAGISGHTAALSARRKLGKEHEVVVVSPNAYYQWVPS
                     NIWVGIGLMKVKQVIFELAPVYKKNHIVFKQALALSIHPEGNAETDKGFVKIRYVSED
                     RKDREEILEYDYLINATGPKLNFGATEGLGPEFYTDSVCTYQHAALTWEHLQACFAKM
                     EKGERQRIVVGTGHPLATCQGAAFEFALNIAFEVARRKLDHLAEISWISNEYELGDFG
                     MGGAFIKRSGYITPTKILAESLFTEYGITWTVGAGVKKIEPGIIHYETLAGEELIKEF
                     DFAMLIPGFAGVGLKAYNKSGDDITTVLFAANGFLKVDADYTLKPYSEWKPSDWPSIY
                     ENPTYNNVFATGIAFAPPHAISKPMVSTKGTAISATAPRTGMPSGVIGKVVAENIADR
                     IKTGRREYKHKASMARMGAACVISAGFGIKKGKAASMTVFPIVPDWKKYPKWGRNLRY
                     TLGEIGLAGHWFKLIMHYMFLYKAKANPFWWLIPE"
     gene            2491..2649
                     /locus_tag="PLI30_11545"
     CDS             2491..2649
                     /locus_tag="PLI30_11545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI80221.1"
                     /translation="MDNKNTIPYSLESFATVPTGWTKFWRTCILYQTVRFFVIGFKVM
                     RIVAGGHS"
     gene            complement(2658..>3490)
                     /locus_tag="PLI30_11550"
     CDS             complement(2658..>3490)
                     /locus_tag="PLI30_11550"
                     /inference="COORDINATES: protein motif:HMM:NF039501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF5686 family protein"
                     /protein_id="HOI80222.1"
                     /translation="QNSGIKFEDISVNYFKDYYVKLFNDYELFNGFVSTIGIEYHIRQ
                     PKKNGQPLLSPVTLESNPIENILVTKKAFAPYLRLSWTPEQYYRYEGRQKIYVRSRFP
                     TFKIELSRSYRNVFGSTSQYNRVEFDMSQNIPLGPMSSFKYRVGAGRFVNQSTEYFAD
                     FTFFAKNNFPDNWQDGIGGTFSLLNRHLYNASDSYVQGHIMLETPFLIIRNIPLISDY
                     INRERLYISQLYTPYIVSYTEFGYGVGNRFFKTANAAIFCSFHKTKFREIGVKASFEL
                     "
BASE COUNT         1013 a          710 c          823 g          944 t
ORIGIN      
        1 cagcagtaag cttaaaggta atgttattcc cggagtagct atagctacag cacttatgcc
       61 gcctatctgt actgccgggt acggattggc cacgctacaa ttcaactttt tcttcggagc
      121 cctgtacctc ttcacgatca atacggtttt tattgccttt gcggcattgg ttgtgtgtca
      181 atacctgaag tttcccatcg gaagcattgt cgatcccgac agaaagaagc gggtaaatcg
      241 agcccttaca gccgtaattc ttatcacgtt agttccgagt gtttattttg gatatcaact
      301 cgtgcagaat gaaaatttta gccagcatgc cgaatatttt atcagtcagg tttctgtagt
      361 ggaggggaat tacctccaaa aaagcaacat taatccatca aataagaatg tggatctggt
      421 gtataatggc tacggacttt ccccgaaaca catcgattcc attaaactgg tggctttttt
      481 taataaaatc gatacagcaa aattaagcat cagtcagggt tttgaggctt ccttcgtaca
      541 ggaaaataca agaagatatc agtcggagac cgaagtcctt aacaacagac tgaatgctac
      601 aacgtttgcc ctgaagcaag ctgaaaacag aatagacagc attcggaata ttcctgcttt
      661 gggagaaaag ctgttatcgg aaatacaacc gctgtatccg cagataagcg gttgttcgta
      721 tacggaaact ttttcttact ttatgcgaaa agacagtatt cttcgcagca acagaacccc
      781 gcttgttatt tttaccgtgt cccgaaacac attgcgtact gccgataaga acaagatagg
      841 ggaatggctc aaaagccggt tggagaaacc ggaagtgctt acctattacg atgaaatacg
      901 ataaacgaaa aaaagagtgc ttaagtcgtt ataaacaaaa gttttattgc tacatttgca
      961 gcaatatgtc ttaatcttca atgtttaact aatccaagaa atactatggc aagattactc
     1021 atcttaggcg ccggtatatc cggccacaca gcggcattgt cggcaagacg gaaattggga
     1081 aaagaacacg aagtagtggt tgtcagccca aatgcctact accagtgggt tccttccaat
     1141 atctgggttg gaatcggttt aatgaaagta aaacaagtta ttttcgaact ggctccggtt
     1201 tataaaaaga accacattgt ttttaagcag gcgctggcgc tctccattca tcccgaagga
     1261 aatgcggaaa ccgataaggg ttttgtgaaa atcaggtatg tttctgaaga tagaaaagac
     1321 cgggaggaaa tcctggaata cgattacctg attaacgcta ccggacccaa gcttaatttt
     1381 ggagccaccg agggtctggg gccggaattt tacaccgatt ccgtatgtac ttatcaacat
     1441 gcggccttga cgtgggagca cctacaagcc tgttttgcca aaatggaaaa aggcgaaagg
     1501 cagcggatag ttgtcggaac gggacatcct ctggccactt gtcagggagc ggcttttgaa
     1561 tttgcgttaa acatcgcatt tgaggtagcc cggaggaaac tcgatcatct ggcagaaata
     1621 agttggatct ccaacgaata tgaattaggc gatttcggta tgggaggggc ttttataaaa
     1681 cgaagcggtt acattacccc taccaagatt cttgccgagt cgctcttcac tgagtatggc
     1741 attacctgga ctgtgggcgc gggagtcaag aaaattgaac cgggaataat ccattacgag
     1801 acactggcgg gagaagaatt gatcaaagag tttgattttg ccatgctcat ccccggtttt
     1861 gcaggagttg gtttaaaggc ttataacaaa agtggagacg atataacaac agttctgttt
     1921 gctgcaaacg ggtttctcaa ggtggatgcc gattatacgt taaaacccta cagtgaatgg
     1981 aaaccatcgg attggccttc tatctacgag aaccccactt acaacaatgt ttttgctact
     2041 gggattgcgt ttgctcctcc gcatgctatt tctaaaccca tggtgagcac gaaaggaacg
     2101 gcaatctcag ccactgctcc ccgtacagga atgccttcgg gggttatcgg aaaagtggtt
     2161 gctgaaaata ttgccgacag gattaaaaca ggcagacgcg agtacaaaca caaggcttcc
     2221 atggcacgta tgggggctgc ctgcgttatc tctgccggat ttggaataaa gaaagggaag
     2281 gccgcatcaa tgacggtttt tcctattgtc cccgattgga aaaaatatcc gaaatggggc
     2341 aggaacctcc gttatacctt aggcgaaata ggacttgctg gacattggtt taagctgatt
     2401 atgcactata tgtttttata caaggcaaag gcaaaccctt tttggtggct gattcctgaa
     2461 tgatttcgat aaccattaga aaacacggat atggataata aaaatacaat accctatagc
     2521 ctggaatcgt ttgccacggt tcccacaggc tggacaaaat tctggcgaac ctgcattttg
     2581 tatcagacag tgcggttttt tgtcataggc tttaaagtga tgcgtatagt agcaggaggc
     2641 cactcgtaag cagtaggtta cagctcgaaa gatgccttga cgccgatttc cctgaatttg
     2701 gttttatgaa acgagcagaa aatcgcagca tttgcagttt tgaaaaaccg gttgccgaca
     2761 ccgtatccaa attcagtgta cgaaacaatg tagggggtat aaagctggct tatgtacaaa
     2821 cgttcccggt tgatgtaatc agagattaaa ggaatgttcc ggatgataag aaaaggggtc
     2881 tcaagcataa tatgtccctg cacataagag tcggaagcgt tgtacaggtg gcggttcagc
     2941 aaactgaatg tcccacctat gccgtcttgc cagttgtcgg gaaagttatt tttggcaaag
     3001 aaggtaaaat cggcaaaata ttcagtgctc tggttaacaa accgtcctgc acccactcga
     3061 tacttaaatg aactcattgg cccgagagga atattttggc tcatgtcgaa ctccacccgg
     3121 ttgtattgtg atgtgctacc aaaaacattc cggtagcttc ttgagagttc tatcttgaaa
     3181 gtgggaaacc gggagcggac gtaaattttt tggcgtcctt cataacggta atattgttcc
     3241 ggagtccagc tgagcctcag gtaaggcgca aacgccttct ttgtgacaag gatattttct
     3301 atcggattac tttctagggt gacaggggat aataacggtt gaccgttttt tttgggttgt
     3361 cggatgtggt attcaatgcc gatcgtagat acaaaaccgt tgaatagttc atagtcgttg
     3421 aataatttca cgtaataatc cttgaagtaa tttacggaaa tatcttcgaa cttgataccg
     3481 ctgttttgca
//
LOCUS       DAOSDR010000342         3485 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrimonas sp. isolate AMR_MDS_1826
            NODE_9517_length_3485_cov_3.937901, whole genome shotgun sequence.
ACCESSION   DAOSDR010000342 DAOSDR010000000
VERSION     DAOSDR010000342.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863355
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrimonas sp. (wastewater metagenome)
  ORGANISM  Petrimonas sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Dysgonomonadaceae; Petrimonas.
REFERENCE   1  (bases 1 to 3485)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3485)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:10:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,720
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 35
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3485
                     /organism="Petrimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9517_length_3485_cov_3.937901"
                     /isolate="AMR_MDS_1826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2023866"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1472
                     /locus_tag="PLI30_11555"
     CDS             <1..1472
                     /locus_tag="PLI30_11555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006843592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycoside hydrolase family 92 protein"
                     /protein_id="HOI80223.1"
                     /translation="MEGKHLGFFTEFATKDREEVLLKAGISFVSLEGAEKNLQAEISD
                     WDFDRIHVLARELWNEALEKISVEGGTKDEKTIFYTALYHTLIDPRTFTDVDGNYPGG
                     DGKIRQRTTFTKRTIFSGWDVFRSQFPLQTIINPEVVSDMINSLVTLAEESGNDYLER
                     WEFLNAYSGCMIGNPAVSIITDAYAKGIRNFDIAKAYTYSRQTVEKFGNGEKGFSDGI
                     STTLEHAYFEWCLSQLAHHLNKPEDEKLYAVRAQSYRNIFDPSVNWFRPKKGSGDWLP
                     WPEKGRLTQNYGCVESNPYQQGWFVPHDLEGMANLMGGREKVIKDLISFFENVPEDML
                     WNDYYNHANEPVHHVPFLFNRLGAPWLTQRWTREICSRAYHNSVEGLVGNEDIGQMSA
                     WYVLAASGLHPVCPGETRLEITSPVFSKITIRLDPRYAQGKTFTIEAQDNSVKNRYIQ
                     GATLNGKTWNKCYIDYADIIRGGTLTLQMGAGPNKEWGN"
     gene            1632..1943
                     /gene="rplU"
                     /locus_tag="PLI30_11560"
     CDS             1632..1943
                     /gene="rplU"
                     /locus_tag="PLI30_11560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005861907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HOI80224.1"
                     /translation="MYVIVDIQGQQFKVQQDQKLFVHRINADQGSEVEFGKVMLIDND
                     GDVQVGAPVIEGAKVIVEVLSHVKGDKVLVFKKKRRKGYRKLNGHRQQFSEVRVKEII
                     A"
     gene            1964..2233
                     /gene="rpmA"
                     /locus_tag="PLI30_11565"
     CDS             1964..2233
                     /gene="rpmA"
                     /locus_tag="PLI30_11565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005861909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HOI80225.1"
                     /translation="MAHKKGVGSSKNGRESESKRLGVKIFGGETTKAGNILVRQRGTV
                     HHPGENVGIGKDHTLFALIDGVVVFRKRHNNRSFVSVLPKVEAEA"
     gene            2344..>3485
                     /locus_tag="PLI30_11570"
     CDS             2344..>3485
                     /locus_tag="PLI30_11570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007660836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOI80226.1"
                     /translation="MKLTQQQKNFIRQNEQHDVRELALKLDNGKQRELNVEFILKQIS
                     GRQTAKEKFPFFYANDEIIYPVHLSLEQASSEATANYKVSLIPDGYKTFVDLTGGMGV
                     DFTILSQRFILSVYVEQDPELCEIAANNFQTLGLQGFVVKNEASETFVEKMSLTDMVF
                     LDPARRDDAGRKVIRIEDCSPDVSEIQNALLTKAKLVVIKFSPMLDISSAVRSLKKVS
                     EVHVVSVENECKELLFLLSSQAEDTVYYAVNLNKGGNIEVFSFTPEEERSLEIPFTRT
                     IGKYLYEPNASILKAGAFKSVAQQYAVQKLHVNSHLYTSNNQISTFPGRVFEVKEWFV
                     PNKKNVKSFLSLTKKANIAVRNFPLSVAEIRRKTGLREGGDVYLFAT"
BASE COUNT         1041 a          720 c          847 g          877 t
ORIGIN      
        1 agatggaagg aaagcacctg ggttttttca ctgaatttgc caccaaagac cgggaagagg
       61 ttttgctgaa agccggcatc tcgtttgtca gcctggaggg ggccgaaaag aacctgcagg
      121 cggaaattag cgactgggat tttgaccgaa tacatgtcct tgccagggag ttatggaatg
      181 aagcacttga aaagattagc gtggaagggg gcaccaaaga tgaaaaaacg attttctata
      241 ccgcccttta tcacaccctg atcgatccgc gaacatttac cgatgtggac ggaaactatc
      301 ccgggggtga cgggaaaatc cgtcaacgca ccacatttac caaacgcacg attttcagtg
      361 gctgggatgt tttccgaagc cagtttcctc tgcaaacgat catcaacccg gaggtggtca
      421 gtgacatgat caactcgttg gtgacccttg ccgaagaaag cggaaacgat tatctggagc
      481 gctgggaatt tctgaacgct tatagtgggt gcatgatcgg caatccggcc gtttctatca
      541 tcacggatgc ctatgccaag ggaatacgga attttgatat cgctaaagct tatacatact
      601 cccggcagac ggttgaaaaa ttcggcaacg gtgaaaaagg attctctgac gggatttcaa
      661 caaccctcga acatgcttat ttcgaatggt gtctttcgca actggcccat cacctgaata
      721 aaccggaaga cgaaaagctt tacgctgtca gagcacaaag ctaccggaat attttcgatc
      781 cttctgtcaa ttggttcagg ccgaagaaag ggagcggtga ctggcttcct tggcctgaaa
      841 aagggaggtt gacccagaat tacggctgtg tggaaagcaa cccctaccag cagggatggt
      901 ttgtgccgca tgacttggaa ggtatggcca acctgatggg tggccgggag aaggtaatca
      961 aagaccttat ctcctttttt gaaaatgtac cggaagatat gttatggaac gattattaca
     1021 accatgccaa cgaacctgta catcatgtgc cgtttctgtt taaccggctg ggtgctccct
     1081 ggctaactca aagatggacg cgcgaaatct gttcgcgtgc atatcacaac agtgtggagg
     1141 ggttggtcgg aaacgaggat atcggacaga tgtcggcctg gtacgtgctg gctgcatcgg
     1201 gacttcatcc cgtgtgtccc ggtgaaaccc gtctagagat taccagtccg gtgttttcaa
     1261 aaataactat caggttagat ccccggtatg cacaggggaa aacatttacc atcgaagccc
     1321 aagataattc tgtgaagaat cgctatattc agggtgctac cctcaacgga aaaacgtgga
     1381 ataaatgtta tatcgactac gctgacatca tccggggcgg aacattgacg ctacagatgg
     1441 gagccggccc gaataaggaa tggggaaact gaaaaatgaa cttacatatc atcctgtcgg
     1501 taaatttata cttcacatat ttggcaattt cgtaaaaatt atgtactttt gcacctcgat
     1561 tcgctaatag gctcaataag aaggggcaca acgccgctcg cctcaggcat ataacgtata
     1621 agttatttaa tatgtacgta attgtagata tccaaggtca gcagtttaaa gttcagcaag
     1681 accaaaagtt atttgtccac agaatcaatg ccgatcaggg cagtgaggtg gaatttggaa
     1741 aagtgatgct catcgataac gatggcgatg tacaagtggg tgctcccgtc attgaaggag
     1801 caaaagtgat tgtagaagtt ctttcgcacg taaaaggaga taaagttctc gttttcaaga
     1861 agaaaagaag aaaaggttac cggaaactga acggccaccg tcaacagttt tcagaagtaa
     1921 gggttaaaga aattattgct taatcagtta aaatcagaag aaaatggcac acaagaaagg
     1981 tgtaggaagt tcaaagaacg gccgcgaatc ggaaagtaaa cgattgggcg ttaaaatatt
     2041 tggtggagaa accacaaaag cgggcaacat tttggttcgc cagcggggaa cggttcatca
     2101 cccaggagaa aacgtaggaa tcggtaaaga tcatacgctt tttgcgttga tcgacggtgt
     2161 tgtggttttc cgtaaacgtc acaacaaccg ttcattcgta tcggttcttc cgaaagtgga
     2221 agcagaagca taaggctttt gcctgcttga aatcaagaat acttgatcca taaaataata
     2281 attttttagt tgataggaac aacctgctat ttcgatagtc aggttgtttt ttattttcac
     2341 aaaatgaaac tgactcagca gcaaaagaat tttatccgcc agaacgaaca acacgatgta
     2401 cgggaactgg cgttgaaact cgataacggg aaacagaggg aattgaatgt tgagtttatt
     2461 ttaaaacaaa tctcgggacg ccaaacagca aaagagaaat ttcctttttt ttatgccaac
     2521 gatgagatca tttatccggt tcatttatcg ttggaacaag catcgtctga agccactgcc
     2581 aactataagg tttcattgat tcctgacgga tacaaaacct ttgtggatct gaccggcggg
     2641 atgggggtgg atttcaccat tctctcccaa cgatttatac tgtcggttta cgtggaacaa
     2701 gacccggaac tttgtgagat agccgccaat aatttccaaa cacttggcct gcaaggcttt
     2761 gtggtaaaga acgaagcaag tgaaactttt gtagaaaaaa tgtcccttac agacatggtt
     2821 ttcctggatc cggcgcgccg ggatgatgca ggacgcaaag tgatccggat agaagactgt
     2881 tctccagatg tgtctgaaat tcagaatgct ttgctgacca aagcaaagtt ggttgtgatt
     2941 aaattctcac ccatgctcga tatttcatca gcggtaagaa gcctgaaaaa ggtgagtgaa
     3001 gttcatgttg tctccgtgga gaacgaatgc aaagagttgc tgtttttact ttcttcccag
     3061 gctgaggata cggtgtacta tgctgttaat ctgaataaag gaggcaacat tgaagtgttt
     3121 tcctttactc cggaggaaga gcgcagcctc gagattcctt ttaccagaac tatcggtaaa
     3181 tacctttacg aacccaatgc ttccatcctc aaagcgggag ccttcaaaag tgtagcacaa
     3241 cagtatgctg ttcaaaaact gcacgtaaac agccacctgt atacttccaa taaccaaatt
     3301 tccacttttc ccggacgcgt gtttgaagtg aaggaatggt ttgtaccgaa caaaaaaaac
     3361 gtaaaatcat ttctatcact gacaaaaaaa gcgaacatcg ctgtccgcaa ttttccattg
     3421 tccgttgccg agatccgacg aaaaaccgga ttgagagagg gtggggacgt gtaccttttt
     3481 gctac
//
LOCUS       DAOSDR010000343         3476 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrimonas sp. isolate AMR_MDS_1826
            NODE_9549_length_3476_cov_3.335574, whole genome shotgun sequence.
ACCESSION   DAOSDR010000343 DAOSDR010000000
VERSION     DAOSDR010000343.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863355
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrimonas sp. (wastewater metagenome)
  ORGANISM  Petrimonas sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Dysgonomonadaceae; Petrimonas.
REFERENCE   1  (bases 1 to 3476)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3476)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:10:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,720
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 35
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3476
                     /organism="Petrimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9549_length_3476_cov_3.335574"
                     /isolate="AMR_MDS_1826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2023866"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..636
                     /locus_tag="PLI30_11575"
     CDS             <1..636
                     /locus_tag="PLI30_11575"
                     /inference="COORDINATES: protein motif:HMM:NF015692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HOI80227.1"
                     /translation="GVQPDLAGGVEETSNGNGSLMRILPLLFYLLDKPVSERFSLTQQ
                     VSSVTHGHIRSVIACFYYLEFARQIFQGKDKFEIYSNLQSEVSNHLTSLSVDPGEIAR
                     FDRLLKSDIAKLPEEKIYSSGYVLHTLEASIWCILTTHSYPEAVLRAVNLGEDTDTTG
                     AVTGGLAGLLYGISHIPENWLRQIARQDDMENLAERLGDKIAGIDRSVAVD"
     gene            952..1677
                     /locus_tag="PLI30_11580"
     CDS             952..1677
                     /locus_tag="PLI30_11580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI80228.1"
                     /translation="MKKLTVLFSFLLIGLLLNGQSLKRLKNGVLIKENQYLENEYKVE
                     FQVFDVGSVTVEIAQIFRKEFDRNDFNCKAIIQTKEKGKILDELYYENIEAVGSSYGI
                     CFNEKQPDENFLIGSKYGDYAGQLIIIDNRGRIIKKPGGDYFTSTNKKHLISDWYSDL
                     SGITIFDFETGETVYSNELPVFLSKWYENTGKYYAAEWEGNRETNNIYEFNLDNLSLV
                     KTDLDLEKLHQFEEVNLTGCKPN"
     gene            1841..2446
                     /locus_tag="PLI30_11585"
     CDS             1841..2446
                     /locus_tag="PLI30_11585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI80229.1"
                     /translation="MRQIFIVTLLIIPIVMSCEKDDFQNDCRNRFYYYNSEKIMLTEI
                     PYQGSVSFYDTLAAETIHQIMEQYQGIRVLSVLSNSNRAVIAVESENCNAADKLLAAV
                     KSDSRIRNCSKFLVSDKGFTLGITDVFVCRLKSSASPDQIAALINDNQVEIVKADHSG
                     YYLLSVDKNCNGDALDLANKFFESGFFDYAEPEWIANYGTY"
     gene            2536..3309
                     /locus_tag="PLI30_11590"
     CDS             2536..3309
                     /locus_tag="PLI30_11590"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HOI80230.1"
                     /translation="MESSGDLKIKPMGLMPSLLFFGIPAAIFSLAIYYVMQKFHQNGI
                     NELTNFFVSMVSPLALMLLAALVAFKVEGNSFKWSALSKRFRLNRMSKSDWIYTLGLL
                     AVMILIYETLSFTSKWLIQFKLFAPPEFLLPAVDPRLEPTFLLDTFMGIPLKGQWWIA
                     VVYFIFLLFNILGEELWWRGYIFPRQELAFQKWTWVIHGTLWGLFHIFWKWNLIILLP
                     SCLALSFVIYKRKNTWIGIVAHMAFNSAPLIGLIIGIIG"
BASE COUNT         1018 a          672 c          807 g          979 t
ORIGIN      
        1 ggggtacagc ctgatttggc cggtggcgtt gaagaaactt caaacggaaa tggctcgctg
       61 atgcggatcc tgccgttgct tttctatttg cttgacaaac ccgtcagcga acgctttagc
      121 ctgacccaac aggtctcctc cgttacccac ggacatatcc ggtcggtcat cgcctgtttt
      181 tactacctcg aattcgccag gcagattttc caggggaaag acaaatttga aatttacagc
      241 aacctgcaat cggaggtttc caatcactta accagcctgt cggtcgatcc cggagagatt
      301 gcacggtttg accggttgtt gaagagcgat atcgccaaac ttccggagga gaaaatttac
      361 agcagcgggt atgtgctgca cacccttgaa gccagtatct ggtgtattct taccacccac
      421 agttacccgg aagccgtttt gcgggccgta aatttaggtg aagataccga cacaaccggc
      481 gccgtcaccg gcggacttgc cggcctgctt tacggaatca gccatattcc tgaaaactgg
      541 ctgcgacaaa tagcgcgaca ggacgatatg gagaacctgg ccgaacgcct gggtgacaag
      601 atcgccggca ttgaccgttc agtggcggtt gactgaagaa aacgccatga aaaacccgct
      661 ctcccgttgg tgttatcgct gattgttaca gcatcgtttg tgagtaaaag tgataacttt
      721 gaaagcggat gtcaaaaatt caacccaatg aatatgaaga tttcgataaa tcacgctgcg
      781 catggaaggt caatgaacca gattgaaatt gaagcgctgg tagagcttgg gtttggtaaa
      841 aaaagagaca aacaaccgga ataaaacatg agaccaacag cagcaacccg aagcagcaga
      901 caggaatatc cgctccgcag cacgcatcaa ccgttatggc tcatgataac catgaagaaa
      961 ctaacggtat tattcagctt tttattgatc ggacttttac tgaatggaca aagcctgaag
     1021 agacttaaaa acggagtttt aataaaagaa aatcaatatt tggagaatga atataaggtt
     1081 gaatttcagg tttttgatgt aggttcggta acagttgaaa ttgcgcagat attcagaaaa
     1141 gaatttgatc gtaatgactt taattgcaaa gccataattc agacgaaaga aaaaggtaaa
     1201 attttggatg aattgtacta tgaaaatatt gaagcagtag gaagttcgta tggaatctgt
     1261 tttaatgaaa aacaaccaga tgaaaatttt ctgattggtt caaaatatgg ggattatgcc
     1321 ggtcagctaa taatcataga caatagaggg agaattataa agaaaccggg aggtgattat
     1381 tttacctcaa ccaacaaaaa acatctaatc tcagactggt attcagattt gtcgggaatt
     1441 acaatttttg attttgaaac aggtgaaact gtttattcga atgagctgcc ggtttttctt
     1501 tcaaagtggt acgaaaatac aggaaagtat tacgctgcag aatgggaggg caacagagaa
     1561 acaaataaca tttatgagtt taatctggac aacctttctc ttgtgaaaac ggatctggat
     1621 ttagagaagc tacatcagtt tgaggaagta aacctcacgg gatgtaaacc aaactgataa
     1681 aatggaagaa ggtttcgtcc ggccggtgat tgacgtgctt ttcagcctgg cggcaggcag
     1741 gaaaacctgt tttgcaacat aatcagctgt ttgcgccagg ttcgacgcaa cccaactccg
     1801 tgattttcga tctgtaataa agaatgtaaa aagtgatgtt atgcgacaaa tattcattgt
     1861 aacgctcctg attatcccaa tcgttatgag ttgtgaaaaa gatgattttc agaatgattg
     1921 cagaaatcgt ttctattact acaattctga aaaaatcatg ctcactgaaa ttccttatca
     1981 gggttctgtt tcattttacg acacgcttgc tgccgaaacg atacatcaga tcatggaaca
     2041 ataccaggga attcgcgttc tttccgttct gtccaacagt aaccgcgctg ttattgctgt
     2101 tgagtcagaa aactgtaatg cagcagataa gctgctcgcc gcggttaaaa gcgattcaag
     2161 gattaggaac tgtagtaaat tcctcgtttc ggacaaaggt tttacccttg gaattaccga
     2221 tgtatttgtg tgcaggttaa agagtagtgc ttccccggat caaattgctg ccttaatcaa
     2281 tgacaatcag gttgaaatag tgaaggctga tcattccggc tattatctgc tcagcgttga
     2341 taaaaactgt aacggtgatg cattagacct ggccaacaaa ttctttgaaa gtggtttttt
     2401 tgattatgca gagccggaat ggattgccaa ttatgggaca tactgaaaaa caggcgttaa
     2461 aattttgcaa attgcattgc ggggacgtat tggctcggga cgtagtgtgt tcaccatttt
     2521 aaattagtct tagcgatgga gagctcaggt gatttgaaaa tcaaaccgat gggattaatg
     2581 ccctctcttt tgttttttgg gattccagct gcaattttca gtttagcgat ctattatgtc
     2641 atgcagaaat tccaccagaa tggaatcaat gagttgacga acttttttgt ctctatggta
     2701 agtccattgg cactgatgct gttagccgca ctggttgcct tcaaggtgga ggggaatagc
     2761 tttaaatggt ccgccctttc aaaaagattc agactaaacc ggatgagtaa gtcagactgg
     2821 atatatactt tgggactact tgcagtaatg attctaattt atgaaacgtt atcctttact
     2881 tcaaaatggt tgattcagtt taaattattt gctcctcctg aatttctttt gccagcggtt
     2941 gacccgagac ttgaaccgac atttcttttg gacactttta tgggcatacc tctgaaagga
     3001 cagtggtgga ttgctgtcgt atatttcatt tttttactat tcaatatttt gggcgaagag
     3061 ttatggtggc gtggatatat tttcccccgg caggaattag cctttcaaaa atggacctgg
     3121 gtgatccacg gaaccctttg gggattattt catatttttt ggaaatggaa tctgatcatt
     3181 ctgttgccat cctgtttggc gctttcgttt gtaatttata aacgaaaaaa tacctggata
     3241 ggaattgtag ctcacatggc attcaatagt gcgcctttaa ttggactcat aatagggatc
     3301 atcggataaa ccaaaaaacg gcctgcctct tttgacggag acaggccgtt tgccgaaaag
     3361 cggggagggt tatttttccc agttgtcggt gcggaatgag gaggccggca acccttcggt
     3421 attgaacagt tcgccggtca caaaatcgtc gaaggcgtaa cgtacggcca ccggct
//
LOCUS       DAOSDR010000344         3447 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrimonas sp. isolate AMR_MDS_1826
            NODE_9662_length_3447_cov_3.973467, whole genome shotgun sequence.
ACCESSION   DAOSDR010000344 DAOSDR010000000
VERSION     DAOSDR010000344.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863355
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrimonas sp. (wastewater metagenome)
  ORGANISM  Petrimonas sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Dysgonomonadaceae; Petrimonas.
REFERENCE   1  (bases 1 to 3447)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3447)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:10:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,720
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 35
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3447
                     /organism="Petrimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9662_length_3447_cov_3.973467"
                     /isolate="AMR_MDS_1826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2023866"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..449)
                     /locus_tag="PLI30_11595"
     CDS             complement(<1..449)
                     /locus_tag="PLI30_11595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012056148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold nucleic acid binding domain-containing
                     protein"
                     /protein_id="HOI80231.1"
                     /translation="MKQLKRTTIADALKSAQTGTEVNVKGWVRTRRGNKNVSFVALND
                     GTTINNIQVVIDIAQFGEEYLKPITTGACLNVNGKLVESQGQGQSVEIQATGIEIYGT
                     ADPATYPLQKKGHSLEFLREIAYLRPRTNTFGAIFRMRHHMSYAIHK"
     gene            complement(561..1667)
                     /locus_tag="PLI30_11600"
     CDS             complement(561..1667)
                     /locus_tag="PLI30_11600"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019540596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HOI80232.1"
                     /translation="MATNNNDSRPSRSYGNANNERHSSSNRNYNVERRPQREDGNYRP
                     SYEENYNRNYESNPNSPEGSKKRRPRVGERVSSGAHGGGGERNYNAYGNAPGRPRNAQ
                     PVNQGPKKLVKKKKIFKQIKYKENADPTAPIRLNKYLANAGVCSRREADEFIQAGVVK
                     VNGEVVTELGTKITRADEVMFNDQVVSLESKVYVLLNKPKGFVTTTDDPENRKTVMDL
                     VKAACKERIYPVGRLDRATTGVLLLTNDGDLASKLTHPKYEKRKIYQVWLDKPVAMEH
                     MQAIADGIELEDGEIHADAISYVTEEDLSQIGIEIHSGKNRVVRRIFEKLGYRVLKLD
                     RVYFAGLTKKNLSRGKWRYLSEKEVNMLRMGAFD"
     gene            complement(1775..3118)
                     /gene="purB"
                     /locus_tag="PLI30_11605"
     CDS             complement(1775..3118)
                     /gene="purB"
                     /locus_tag="PLI30_11605"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005829591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0006188 - IMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HOI80233.1"
                     /translation="MKLDELSAISPIDGRYRDKTRELALYFSEYALIKYRTQVEVEYF
                     IALCELGISPLDKVEKPVFGVLRKMAEDFSEQDAQRIKEIEKVTNHDVKAVEYFLKEK
                     FDELHLDAFKEFIHFGLTSQDINNTATPMMWKEALQNTYIPQLEELTALIGKFAEEWK
                     EVPMLARTHGQPASPTRLGKEIQVFEYRLRNQTEVLKRIPAKAKFGGATGNFNAHRVA
                     YSHIDWKEFANSFLSNKLGLVREEYTTQISNYDALAASCDALKRINTILIDFTRDVWQ
                     YISMEYFKQRIREGEVGSSAMPHKVNPIDFENAEGNLGIANAWLEHLAAKLPISRLQR
                     DLTDSTVIRNIGMPLAHGLIAIKSTTKGLHKLLLNEEAIERDLENNWAVVAEGIQTIL
                     RREGYPKPYEALKALTRTNRHITKESISDFIDTLEVNEEIKKELKAISPKNYTGI"
BASE COUNT          721 a          908 c          763 g         1055 t
ORIGIN      
        1 aacttgtgaa tggcatacga catgtggtga cgcatacgga aaatagctcc aaacgtgttt
       61 gtcctcgggc gcaggtaagc gatctcccgc aggaactcca gtgaatggcc cttcttctgc
      121 agcggatacg ttgccggatc tgccgttccg tagatttcga tcccggttgc ctggatctcc
      181 acagactgtc cctgtccctg cgattccacc agcttgccgt tcacattcag gcaggccccg
      241 gtggtgatgg gtttaagata ctcttcacca aactgggcta tatctatgac tacctgtata
      301 ttatttatgg tcgtcccatc atttaacgct acaaagctca catttttatt tccgcggcgg
      361 gtacgcaccc atccctttac atttacttct gttccggttt gcgccgattt taacgcatcg
      421 gcaatcgttg ttcttttcag ttgtttcata ttactcctaa tcccttgtat cctcgtcaga
      481 ttcaagggaa tataatttaa atattcgttt tacagatatc tccccgccat ccggcggacc
      541 ggcctgcagg aggccgggag ctaatcaaat gcacccatcc tcagcatgtt cacctctttt
      601 tccgacaagt aacgccattt tccgcgcgac aggtttttct ttgtcagtcc ggcaaagtac
      661 acccggtcga gcttcagcac ccggtaaccc agcttttcaa aaatacggcg tacaacgcgg
      721 ttttttcccg agtggatctc aatgcctatc tggctcaggt cctcttcggt aacgtagctg
      781 atggcgtcag catggatttc accgtcctcc agttcaatac cgtcggcgat agcttgcata
      841 tgctccatag ccacgggttt atccaaccat acctggtaga ttttccgttt ctcgtacttg
      901 gggtgggtca gctttgatgc caggtcgccg tcgtttgtca gcagcagtac tccggtagta
      961 gccctatcga ggcggccaac aggataaatc cgctctttgc aggcagcttt caccaggtcc
     1021 atgaccgttt tccggttctc gggatcatcg gtagtggtga caaaaccctt gggtttgttc
     1081 agcagcacgt aaaccttgct ttccagcgat actacctggt cgttgaacat cacctcgtcg
     1141 gcacgggtga tctttgtgcc cagctcggta accacctcgc cgttcacctt cacaactccg
     1201 gcctggataa actcatcggc ctcacggcgc gagcaaactc cggcattggc caggtatttg
     1261 ttgagacgga tcggtgccgt tggatcggca ttttccttgt atttgatctg cttgaagatt
     1321 tttttcttct tcaccagctt tttcggtccc tggttcacag gttgtgcgtt acggggcctt
     1381 cccggcgcgt tgccgtaagc gttataattt ctttcacctc ctccaccatg ggcacccgaa
     1441 gagactctct cccccactct cggacggcgt tttttgctac cttcagggct attggggttg
     1501 ctttcatagt ttctgttgta attttcttca tacgatgggc gatagttgcc atcttcccgc
     1561 tgtggtcttc tttctacgtt gtaatttctg tttgaagaag agtgtctctc attgttcgcg
     1621 ttgccataac tccggctggg tcgcgaatcg ttgttgttcg ttgccatttt tcttttttaa
     1681 attttaattt tcaataacct cacggtttcc ttttaaacaa tacctaaaac tttttcgttt
     1741 ccatgttcct tgcagaaaaa agtctttctg tcaatcaaat acctgtatag ttttttggcg
     1801 aaatcgcctt gagttctttt tttatttctt cgtttacctc caacgtatcg atgaagtccg
     1861 aaatgctttc tttcgttatg tgccggtttg tgcgcgtcaa cgctttcagt gcctcataag
     1921 gttttggata gccttcccgg cgcaggatgg tttgaatccc ttctgccacc actgcccaat
     1981 tgttttccag atcccgctct atcgcctcct catttaacaa caatttatgc agcccttttg
     2041 ttgtgctttt tatcgcaatt aatccgtgcg ccagtggcat tccgatgttg cggatcaccg
     2101 ttgagtcggt caggtcccgc tgcagcctcg agatcgggag tttagccgcc aggtgttcca
     2161 gccatgcatt ggcaatcccc aggttccctt cggcattttc aaaatcgatg ggatttacct
     2221 tatgcggcat ggccgatgag cccacttccc cttcccggat acgttgttta aaatattcca
     2281 tcgatatata ctgccacaca tcacgtgtga aatcgataag gatggtgtta atccgcttca
     2341 gggcgtcgca ggaggccgcc agcgcatcgt aattggatat ctgtgtggta tactcctcgc
     2401 gtaccagtcc cagcttgttc gacaggaaac tgtttgcaaa ctctttccag tcgatgtgcg
     2461 aataagccac ccgatgagcg ttgaagttcc ctgtcgcgcc gccgaacttc gctttcgccg
     2521 ggattcgttt cagcacttcg gtctggttcc tcagccgata ttcaaaaact tgtatttcct
     2581 ttcccagccg tgtaggcgat gccggttgcc cgtgtgtgcg tgccagcatg ggcacctctt
     2641 tccactcttc ggcaaacttg ccgatcagtg cggtcagctc ctccagttgc gggatgtacg
     2701 tgttctgtag cgcctctttc cacatcatcg gcgtagccgt attgttgatg tcctgcgagg
     2761 tgagcccgaa atggatgaac tccttgaatg catcaaggtg cagctcgtcg aatttctctt
     2821 tcaagaaata ttccactgcc ttaacgtcgt ggttggtaac cttctctatc tccttgatcc
     2881 gttgcgcgtc ctgttccgaa aagtcttccg ccattttcct cagcacaccg aaaacgggct
     2941 tttccacttt atcgagcggg gagatgccca gttcacaaag tgcaataaaa tattccacct
     3001 ccacttgcgt gcggtatttt atcagtgcat attccgagaa ataaagagcc aattcgcggg
     3061 ttttatccct gtatcgtccg tcaataggag atatggcgga aagttcgtcg agtttcattg
     3121 ttaacgcatt ggttttatag atttcgaaac aggcgaaaaa tcacttccta ccctgcttca
     3181 ctcatcataa ttttgatttt cagtacgtgc cgaatcagta aatttttatc tttcttttta
     3241 ttgagagtac aaaagtagtg ataaatacag tgccgtaaaa atttttgtaa aaatatttac
     3301 gaattatttt gcagcaaacg aaaaacgact tatctttgca tcactttttc agaaagcaac
     3361 ctccgggagc ggacaaaaag cagaagaagt aaccgggcgt ttagctcagc tggttcagag
     3421 catctgcctt acaagcagag ggtcggc
//
LOCUS       DAOSDR010000345         3441 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrimonas sp. isolate AMR_MDS_1826
            NODE_9688_length_3441_cov_3.936503, whole genome shotgun sequence.
ACCESSION   DAOSDR010000345 DAOSDR010000000
VERSION     DAOSDR010000345.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863355
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrimonas sp. (wastewater metagenome)
  ORGANISM  Petrimonas sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Dysgonomonadaceae; Petrimonas.
REFERENCE   1  (bases 1 to 3441)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3441)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:10:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,720
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 35
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3441
                     /organism="Petrimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9688_length_3441_cov_3.936503"
                     /isolate="AMR_MDS_1826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2023866"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..328)
                     /locus_tag="PLI30_11610"
     CDS             complement(<1..328)
                     /locus_tag="PLI30_11610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018462825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI80234.1"
                     /translation="MRVSYFILIILLLGCNNPANKNRINRKEVVSRHNIVSEKFSKRS
                     PAQVGNGEIAFGVDITGLQTFVPFNTLSQWGWHSDPVPEGVDISNFKGQQVLHNGRMV
                     TYPLRNE"
     gene            complement(340..2670)
                     /locus_tag="PLI30_11615"
     CDS             complement(340..2670)
                     /locus_tag="PLI30_11615"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009135396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH92 family glycosyl hydrolase"
                     /protein_id="HOI80235.1"
                     /translation="MIYKTMSMCNKTIITLLCAVSVFGSLSAQQKTEDLTQYVNPMIG
                     TQEMGHVFPGSTVPFGMVQLSPDTDTIPYAVDGKYTGTVYRYCAGYQYNDPTIVGFSH
                     THFSGTGHSDLGDFLIMPTTGKLQLNPGTSDKPENGYRSRFSHETETATPGYYRVTLD
                     DYNIDAELTTTEHVGFHQYTFPESEDAHIVLDLTHGIYNYDGKVLWSQVWVHNDTLVT
                     GYRITSGWARTNYLYFAMVFSKPIENYGSRNEEELVYRGFWRKFDQENNFPEMGGRKL
                     KTHFDFKTEAGEKIKIKFALSAVSTEGALKNLYAEVPHFDFDRVKNEAKEKWQKELER
                     IRIKATPEKKETFYTSLYHTFINPVEYMDVDSLYRGIDHQIHKAEGFVNYSVFSLWDT
                     YRALHPLLTIIQPERTSDMVNSMLAHHDQSVHKSLPVWSHFGNENWCMIGYHAVPVIA
                     DAWVNGIRGFDPQRALEASVSSATYPNYGNLDDYMKLGYVPFDKSVYGSSMTLEYAYD
                     DWAIAQLAESIGNKEVAAKFNERAHNWKNLFNNNTGFVGAKNSDGSWKAPFDPLHTAN
                     EGFIEGNSWNYSLYVPHDVPALIQRMGGNERFSQYLDTLFTMYLPDKYFAETEDVTRE
                     GLIGCYVHGNEPSHHVPYLYNWAGKPWKTQERIHQIKNSMYLNKPDGLCGNDDCGQMS
                     AWYVFSSLGFYPVAPASGQYAIGSPSVKEATLQLPNNKELVVKVENYDEKNIYVQHVE
                     INGKPVRDYTLLHRDLIKGGELLFKMGRKPMKKRFK"
     gene            complement(2679..>3441)
                     /locus_tag="PLI30_11620"
     CDS             complement(2679..>3441)
                     /locus_tag="PLI30_11620"
                     /inference="COORDINATES: protein motif:HMM:NF024627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HOI80236.1"
                     /translation="NEIFNAPHVAILLSGNDHLIEYNEIHHVCMETDDAGAIYYGRNP
                     SERGHLVQYNFIHHLPERYRTTAIYHDDAACGMKVHGNVFYKAGAFPVLIGGGSDNPY
                     TNNIFIDCPVGIKVDNRLQAFDWAKPMIAPGGIIEQRLNEIKFDRPPYCTNYPELAKY
                     WEEDPSFPKRNRVDRNLFVNVGQTVLKVDDGVNADKQFLDFTTNNLITREDPGFIDKN
                     RMNFKLTETSAVFEKIRGFEAVPFEKMGTYAIGNK"
BASE COUNT          801 a          811 c          811 g         1018 t
ORIGIN      
        1 cctcgtttct gaggggatag gtgaccatcc ttccgttgtg aagtacttgc tgtcccttga
       61 aattgctaat atccacacct tcgggtaccg ggtccgaatg ccatccccat tgcgacaggg
      121 tattgaaagg aacaaaagtc tgtaatccgg tgatatccac tccaaaagcg atttcaccgt
      181 ttcccacttg tgccggactg cgtttgctga atttttcgga tacgatgttg tgtcgcgaga
      241 ccacctcctt gcggttgatt ctgttcttat tagctggatt gttacagccc aatagcagga
      301 tgatcagtat aaaatagctt accctcataa ggattcgtat tatttaaatc ttttcttcat
      361 cggtttcctg cccatcttga acagcagctc accccctttg atcaggtctc tgtggagcaa
      421 ggtgtagtcc ctgactggct tgccgtttat ttccacatgc tggacgtaga tgtttttttc
      481 atcgtaattc tccactttga ctaccagttc cttgttgttc ggaagttgca gggttgcctc
      541 tttgacggag ggtgagccaa tggcgtattg tcccgatgcg ggtgcaaccg ggtagaagcc
      601 cagcgaagag aaaacatacc aggcagacat ctggccacag tcatcgttgc cacacaatcc
      661 gtcgggtttg ttcaggtaca tgctgttttt gatctggtga attcgttcct gtgttttcca
      721 gggtttgccg gcccaattgt agaggtaagg cacgtgatgg ctaggttcgt tgccgtgcac
      781 gtagcaacca atcaatccct cccgggttac atcttccgtt tcggcaaaat acttatccgg
      841 caggtacatg gtaaaaaggg tgtccaggta ttgcgaaaaa cgttcatttc cacccattct
      901 ctggatgagg gcaggtacat cgtgcggtac atagagcgaa taattccatg aattgccttc
      961 gataaaccct tcattggccg tatggagtgg atcaaaggga gctttccagc ttccgtcact
     1021 gttctttgca cccacaaacc cggtgttgtt gttgaacagg tttttccagt tgtgagcccg
     1081 ttcgttgaat tttgcggcta cctccttgtt gccgatcgat tccgccagtt gagcaattgc
     1141 ccagtcgtca taggcatatt ccagggtcat ggaggagccg tatacacttt tgtcaaacgg
     1201 cacatatccc agtttcatgt agtcatcgag gttcccgtag ttggggtagg ttgccgagga
     1261 taccgatgcc tccagtgccc gctgcgggtc aaaaccccgg ataccgttta cccaggcgtc
     1321 agcgatcacc ggcacggcat gataaccgat catgcaccag ttttcattac caaaatgact
     1381 ccataccggc agggatttat gtacactttg gtcatgatga gccagcatgg aattaaccat
     1441 atcggaagta cgttccggtt gtatgattgt caacaacgga tgcagtgccc ggtaagtgtc
     1501 ccacaaggag aaaacagagt aattgacaaa tccttccgcc ttgtgtatct ggtggtcgat
     1561 tcccctgtac aggctatcta catccatgta ttcaacagga ttgataaacg tgtggtacaa
     1621 tgaagtgtag aatgtttctt tcttttccgg tgtagcttta atcctgattc tctccagttc
     1681 cttttgccat ttctctttgg cttcattttt cacccggtcg aaatcaaaat gaggcacttc
     1741 ggcatagagg tttttcagtg ccccttcggt gctgacagca gaaagggcaa atttgatctt
     1801 gattttctct cccgcttccg ttttgaagtc gaagtgggtc ttcagttttc tgccacccat
     1861 ttcagggaaa ttgttttcct ggtcaaactt acgccagaaa ccgcgataaa ccagctcttc
     1921 ttcattgcgg gacccgtaat tttcaatggg cttggaaaag accatggcaa aatagaggta
     1981 attggtccgc gcccatccgc tggtgatgcg gtagcctgtc accaatgtgt cgttgtgtac
     2041 ccatacctgt gaccacaata cttttccgtc ataattgtag attccgtgtg tcaagtcaag
     2101 gacgatgtgc gcatcttcac tttctggaaa ggtatattgg tgaaaaccca catgttcagt
     2161 agtggtcaac tcggcatcga tattgtaatc gtccagtgtt acgcgatagt agccgggtgt
     2221 agctgtttcg gtttcgtggc tgaatcgcga ccggtatcca ttctccggtt tgtcggacgt
     2281 gccggggttc aactgcaatt tcccggtagt gggcataatc agaaaatccc ccaggtcgga
     2341 atggcccgta ccactgaaat gggtatggct gaatcctact atggtggggt cgttgtattg
     2401 ataaccggca cagtaacggt acacggtgcc tgtatatttc ccatctacgg catacgggat
     2461 ggtatcggtg tccgggctga gttgtaccat accaaaaggg acggtagaac cgggaaatac
     2521 atgccccatt tcctgagtgc cgatcatggg attcacatat tgcgtcaggt cttcagtttt
     2581 ctgttgtgcg gataatgacc caaagacgga tactgcgcac agaagtgtaa taattgtctt
     2641 gttgcacatg ctcattgttt tataaatcat attgttgatt acttattacc gattgcatag
     2701 gtacccattt tttcaaaagg aacggcttca aagcccctga ttttttcaaa tactgctgaa
     2761 gtttccgtta acttgaaatt catcctgttt ttatctataa atcccggatc ttcacgggtg
     2821 ataagattat tggtggtgaa atcgaggaat tgtttatccg catttacccc atcgtctact
     2881 tttagtacgg tctgtccaac atttacaaac aggttcctgt ccacccggtt cctttttgga
     2941 aaagagggat cctcttccca gtattttgcc agttccggat agtttgtaca gtagggaggc
     3001 cgatcgaatt ttatttcatt caatcgctgt tcaataatcc ctccgggagc gatcatcggt
     3061 tttgcccaat caaaagcctg taagcgattg tctactttaa tcccgaccgg gcaatcaatg
     3121 aaaatgttgt ttgtgtaggg attgtcacta cctccgccaa tcaatacggg aaaagcgccc
     3181 gccttgtaga acacgttccc gtgcactttc atcccgcagg ccgcatcatc gtgatagatg
     3241 gctgtggtac gatagcgttc cggcaggtga tggatgaaat tatattgcac caggtgtcct
     3301 ctttcagagg gattacggcc gtaataaatt gcacctgcgt cgtccgtctc catacaaaca
     3361 tgatgaatct cgttatactc aatgaggtga tcatttccgg agagtagaat ggctacatgc
     3421 ggagcattaa atatttcatt a
//
LOCUS       DAOSDR010000346         3430 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrimonas sp. isolate AMR_MDS_1826
            NODE_9726_length_3430_cov_3.682074, whole genome shotgun sequence.
ACCESSION   DAOSDR010000346 DAOSDR010000000
VERSION     DAOSDR010000346.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863355
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrimonas sp. (wastewater metagenome)
  ORGANISM  Petrimonas sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Dysgonomonadaceae; Petrimonas.
REFERENCE   1  (bases 1 to 3430)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3430)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:10:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,720
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 35
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3430
                     /organism="Petrimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9726_length_3430_cov_3.682074"
                     /isolate="AMR_MDS_1826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2023866"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1246)
                     /locus_tag="PLI30_11625"
     CDS             complement(<1..1246)
                     /locus_tag="PLI30_11625"
                     /EC_number="3.4.21.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018361041.1"
                     /note="PtrB; oligopeptidase that cleaves peptide bonds
                     following arginine and lysine residues; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptidase B"
                     /protein_id="HOI80237.1"
                     /translation="MKSWNYILSIVAAALMTACNPSKKETVMLKQSDFPAPPVAEVIP
                     DTFSNFGQTRIDNYYWLKDKNNPKVIDYLKAENAYTDTVMASTKALQQTIYDEIIGRI
                     KEDDESYPSFSDGYYYYSRTEKGKQYRTYCRRKGTLDAPEEIIFDVNKMAEGKPAYIF
                     RGYSISPDNSKAAYFYNETGSYAEFIMKVKDLASGKDVGFTVNGATSVAWANDNKTLF
                     YGIVDNTLRSYRILRQVLDGSRADLVYEETDARFSTYVSGSKTKQYIFISSASSTTAD
                     ERYIPADKPLEQFKVFLPRVQDVEYSVYPHKEKFFVRYKDKENLNGKIYETPLTSHED
                     RNTWKEFLAHDKNVRIEGIDILRDYVTIELRRNGLSEIIAKPASGIGEIKTIAFPEPV
                     YSAGLNGNPEYDATTFRYSYTSL"
     gene            1466..2356
                     /locus_tag="PLI30_11630"
     CDS             1466..2356
                     /locus_tag="PLI30_11630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HOI80238.1"
                     /translation="MQHHNHTHHNEDVKNIRVAFFLNLLFTVIEIVGGILTNSVAILS
                     DAVHDLGDSFSLGLSWYFQKIAKRPRTRNYTYGYKRFSLMGAVINSVILTVGSVLILM
                     NAIPRLFNPQQPDVKGMLLLAVLGVVVNGAAVLKLRKGRSLNEKVVSLHLLEDVLGWL
                     AVLLGAGVMYFVDAPFIDPLLSIAISVFILFNVYRNIRESLRIILQGSPDLINIDEIE
                     KTVMALMNVKNVHDLHVWSVDGEYNVLTIHVVLTKPLSMAELHSLKKEIRRRLFSLNV
                     QHSTIEFETPDEDCELEECC"
     gene            complement(2406..>3430)
                     /locus_tag="PLI30_11635"
     CDS             complement(2406..>3430)
                     /locus_tag="PLI30_11635"
                     /inference="COORDINATES: protein motif:HMM:NF012516.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004650 - polygalacturonase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyl hydrolase family 28 protein"
                     /protein_id="HOI80239.1"
                     /translation="ILDNCTIQLSDECRDNMFRSENVGTGIEHPVWLNNIHLIGVGKV
                     LLKGADNPRATGDAYRTLVMGTGKGRISYGSDAGKKGEKQRGDWRNNLIQIALVDEFS
                     IKNVTIQNSHAWAMSFERTKNAEISAIRFNNPEIIEINGKKVSVYNKDGIDLRHGCKY
                     FKIDDISGLTGDDFIALSSLDIEPYYHSNGDINSYQVTSTRWNGPEDDTEQIFITNCR
                     TNYPGVAIRASDNASIHHVYIDGIVVTNRPDSQAPYMGSPYTLYIGGRGYGKLSIAGK
                     INNIYASNLTGDGKSLITIEAPITDCLFMNGIYTGTAPSAITYKIDKSETKNIIEVNL
                     IKTSKD"
BASE COUNT          837 a          717 c          761 g         1115 t
ORIGIN      
        1 tgagtgatgt ataggaataa cggaaagtgg tggcatcgta ttctggattt ccgtttaatc
       61 ctgccgagta aaccggctca ggaaaagcta tggttttaat ttcccctatg cctgaagcag
      121 gtttcgcgat aatctcactc aacccatttc tgcgcagttc gattgtcacg taatctctca
      181 ggatatcgat accttcgatc cgaacgttct tgtcgtgtgc tagaaactct ttccaggtgt
      241 tgcggtcttc atggcttgtc aagggtgttt cgtaaatttt tccgttcagg ttttccttgt
      301 ctttgtatcg gacaaaaaac ttctccttgt gcggataaac tgaatattcc acgtcttgga
      361 cacggggcag aaacaccttg aactgttcga gcggtttatc tgcaggaata tagcgttcgt
      421 cggcggtagt ggagcttgcc gatgagataa aaatgtattg tttggtcttg ctccccgaaa
      481 cgtaagtaga gaaccgtgcg tccgtttcct cgtataccag gtcggccctg gaaccatcca
      541 gtacctgacg taaaatgcgg taggagcgta acgtgttgtc gacaataccg taaaaaagtg
      601 tcttgttatc gtttgcccag gccacggaag tagcgccgtt aaccgtaaat ccaacatcct
      661 tgcccgaagc aaggtctttc actttcataa taaattccgc ataggaaccg gtttcgttgt
      721 agaaataggc tgctttgctg ttgtcgggac taatggaata tccccggaaa atatatgccg
      781 gcttcccttc cgccatttta ttgacgtcga aaataatttc ttcgggcgca tccaatgtgc
      841 ctttgcgccg acaataggtc cggtattgtt tccccttttc ggtgcggctg taataatagt
      901 atccgtcgga aaacgacggg taactttcat cgtcttcttt tatccggccg ataatttcgt
      961 cgtaaatggt ttgttgcaac gcttttgttg atgccataac cgtatcggta taagcattct
     1021 ccgctttcag gtaatcaatc accttcggat tatttttgtc cttcagccaa taataattat
     1081 cgatacgcgt ttgcccgaaa ttagagaatg tgtcgggaat tacttctgct accggtggtg
     1141 cgggaaaatc tgattgtttc aacatgacag tttctttttt cgaagggttg caggcggtca
     1201 tcaacgctgc agcaactatt gaaaggatat aattccagct tttcattatt gttggattta
     1261 ttcgtttaca atatggagtt caatttatat gcttttttgt cagcattttt tagaaaaaca
     1321 gtatcccggt tgagccggcc gtataaaaat tgtttgttta tatggatttt aaacgatgca
     1381 aatgtatgat ttattattga taattgtgtt ttatgaacaa aaaaattatc cttatgttat
     1441 atcaaataac tcatttaaca agtaaatgca acaccacaat catactcatc acaacgagga
     1501 cgtcaagaac atccgggtgg cattttttct taatctcctt tttacggtaa ttgagatcgt
     1561 aggtggcata ctgacaaaca gtgtcgctat cctgtctgat gcggtacatg atttaggcga
     1621 cagtttttcg ctgggcttgt cgtggtactt tcagaaaatt gccaagcgtc cgcgtaccag
     1681 gaactacacc tacggttaca aacgcttttc cctaatgggt gctgtgatca actcggtgat
     1741 attgactgtg ggcagcgtgc ttattttgat gaatgccatt ccgcggctgt tcaacccgca
     1801 gcaacccgat gtaaagggaa tgcttttact tgccgtgctg ggtgttgtgg tgaacggcgc
     1861 agcagtgctg aagctgcgta aaggacgctc attgaacgaa aaagtagttt cgttacatct
     1921 gcttgaagat gtactgggat ggctggccgt ccttcttggt gccggcgtca tgtatttcgt
     1981 ggatgcaccg tttatcgatc cgctgctttc cattgccatt tcagtgttta ttcttttcaa
     2041 cgtataccgg aatattcgtg agagtttacg gattattttg caaggcagtc ccgatttgat
     2101 caatatcgat gagattgaaa agacggttat ggcgcttatg aacgtaaaaa atgttcatga
     2161 cctgcatgtc tggtcggtgg acggagaata taacgtactg accatccatg tagtgcttac
     2221 aaaaccgttg tccatggctg aactgcatag tctgaagaaa gagatccgga ggagactgtt
     2281 ctcgctaaat gtacaacatt ctaccatcga gtttgaaaca cccgatgaag actgtgagct
     2341 ggaggagtgt tgctaaagca tgaagtcgct tcgcaagccg tttgcttatt ttatacattg
     2401 ttttttcaat cttttgaagt ttttatcaag ttgacttcga taatattctt tgtttccgat
     2461 ttatctattt tatacgttat cgccgaaggt gctgttccgg tataaattcc attcataaat
     2521 aagcaatcgg ttatgggagc ttcaatagta atcaacgatt tcccgtctcc tgttaaattg
     2581 gatgcataga tattgttgat ttttccggca atggataatt taccgtatcc ccggcctcct
     2641 atgtataggg tataagggct tcccatatac ggtgcctggc tgtcaggcct gtttgttaca
     2701 acaattccgt cgatgtatac gtgatgaatg cttgcgttat cgctggcacg tattgctact
     2761 cccggataat tggtccggca gtttgtgatg aatatttgtt cggtgtcgtc ctcgggaccg
     2821 ttccagcggg tagaggtcac ctgatacgag tttatatctc cgtttgagtg ataataaggt
     2881 tcaatatcca aacttgataa agcgataaaa tcatcaccgg ttaaaccgga aatatcgtct
     2941 atcttaaagt atttacatcc atgccttaaa tctataccgt ctttattata tacactcact
     3001 ttttttccgt taatttcaat gatttccgga ttattgaaac ggatggccga tatttcggca
     3061 ttttttgtcc tttcaaaact catggcccag gcatgggagt tttgaatcgt gacatttttt
     3121 atggaaaatt cgtccactaa ggcaatctga atcaggttgt tcctccagtc gcctcgttgt
     3181 ttttcgcctt tctttccagc gtcactgccg tagcttatcc gtcctttgcc ggttcccatt
     3241 accagtgtgc ggtaggcatc tcctgtggcg cggggattat cggctccttt cagcaggact
     3301 tttcctactc cgataaggtg tatgttattc aaccatacag gatgttcaat tcctgttcct
     3361 acattttcgc ttctgaacat attgtcgcgg cattcatcgg aaagttggat ggtgcaattg
     3421 tctaaaatga
//
LOCUS       DAOSDR010000347         3419 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrimonas sp. isolate AMR_MDS_1826
            NODE_9769_length_3419_cov_3.064804, whole genome shotgun sequence.
ACCESSION   DAOSDR010000347 DAOSDR010000000
VERSION     DAOSDR010000347.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863355
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrimonas sp. (wastewater metagenome)
  ORGANISM  Petrimonas sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Dysgonomonadaceae; Petrimonas.
REFERENCE   1  (bases 1 to 3419)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3419)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:10:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,720
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 35
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3419
                     /organism="Petrimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9769_length_3419_cov_3.064804"
                     /isolate="AMR_MDS_1826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2023866"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(102..1520)
                     /locus_tag="PLI30_11640"
     CDS             complement(102..1520)
                     /locus_tag="PLI30_11640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI80240.1"
                     /translation="MGLLGGATLLSPGCIPKKNNKTETASEPAKTLSLKYVRGNIKII
                     GVDSRFEREPLRNPFGFKGGYLSELWQTVSYLRSRNNNHGIGLGTQSVLWSDDLIFSS
                     HSESGGNALMYSLTERALQLLKNQSFNNPTELIEPLYQEVHEYGKSVTNNSDLRKTFA
                     LNALVSVDNALWLLYAKENGITNFDQLIPDEFKTTFSGKHEKLAAIPLISYNVTPEEI
                     KKEVEQGYFFMKIKIGQSGTQSEMLEKDKNRLLEIHNVLKDIRSPYSKTGRLPYYFDA
                     NGRYEAKDTFNRFLDYARQIGAFDQIAMVEEPFPEKMEMDVADVPVRLAADESAHTDK
                     DTIKRIQMGYKAVALKPIAKTLSMTMKIAKAAAERNIPCFCADLTVNPILVEWNKNVA
                     ARLKSFPGLDNLGLVESNGHQNYVNWHQMKKYLPQCRASWVDVKDGFYHTNKEYFNNG
                     GGIFDSIPHYEEMFKKTTGNIS"
     gene            complement(1881..>3419)
                     /locus_tag="PLI30_11645"
     CDS             complement(1881..>3419)
                     /locus_tag="PLI30_11645"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HOI80241.1"
                     /translation="ERIGACIPSSGGTGAEVPWRYNAHKYDVEDIALLSCAQPAWLHP
                     RLRFFVGRENKLPIDQNSFMALIAPRGLMISTATTESASNIFGIEQAYHSSKRVYQFL
                     NAGDNIAITSRYGLHGVNANDIEGYIDFFDFVFNRTDRKPGNRLLHNYSFETWCALSN
                     QSVTPHDHGLATIDLDVYANNRNKWKDSKQIIKNNLRWMLGDEPAGVTNPGPRSLAKG
                     DIGEERFGSFLSRPRETKSMKIMAITPYDGFGDNLFGYLYYPVDETGKLLGEDLPVVI
                     YLHEYDYSKGFSSMSYDHEIQSVFEDLTKLGYGVFAFDMIGFGNRLEEGVNFYDRYPL
                     WSKMGKMVADMKGAVDAISNLDFVDRSRIVVSGYSLGGTVALLSAALDERIAGVVSIA
                     GFTPMRTNTLDRGTEGIKAYSHLHGLIPKLGFFVGHESEIPVDFDQIIGCIAPRPVLL
                     IAPERDKDAHLDDIKKCVGQVGQIYGLYSSKDNIQLYTPNDYNRFSTFMRQKMFYWLK
                     TLKE"
BASE COUNT         1001 a          740 c          593 g         1085 t
ORIGIN      
        1 gtagttttag ttaccccggc gacagatatt ccggcgacag ccatcgatga ttttttgaga
       61 aaatccctac gtgttgtttt gtttttcata aatatctttg tttagcttat atttcctgtt
      121 gtttttttga acatttcttc ataatgaggt attgaatcaa aaattccacc tccattattg
      181 aagtattctt tattggtgtg gtaaaatcca tcttttacat caacccaaga agctctgcac
      241 tgagggaggt attttttcat ttggtgccaa tttacatagt tctgatgtcc gttactttct
      301 accaaaccga gattatcaag gccggggaac gactttaacc gagccgctac atttttgttc
      361 cactctacca aaataggatt aacagtcagg tcagcacaaa agcagggaat atttctttct
      421 gccgcagctt ttgcaatctt cattgtcata ctcaatgtct tagcaatagg tttcaaagcc
      481 actgccttat atcccatctg aatacgttta atagtgtcct tgtcagtatg ggcactctca
      541 tcagctgcta atcgaacggg gacatccgca acatccatct ccattttttc aggaaaaggc
      601 tcttcaacca tcgctatttg atcgaatgcg ccaatctgcc gggcataatc taaaaaacga
      661 ttaaaggtat ctttggcttc gtatctgcca ttagcatcaa aatagtaagg taacctcccg
      721 gtcttggagt atggtgatct tatatctttt aaaacattgt gtatttcaag tagccggttt
      781 ttatcttttt caagcatttc tgactgcgta cccgactgac caatttttat tttcataaag
      841 aagtatccct gttctacctc tttttttatc tcctctggtg taacattata tgagattaaa
      901 ggtattgctg caagtttttc atgtttccca gaaaaggtgg ttttaaattc atcaggaatc
      961 aattgatcaa aattcgtaat tccattttct ttagcataaa gcagccaaag ggcgttatct
     1021 actgagacca gtgcattaag agcaaaagtc tttctcaagt cagaattgtt tgtgactgat
     1081 ttgccatact catgaacctc ttgatataaa ggctcaatca actcagtagg attattgaat
     1141 gattgatttt tcaataactg aagtgctcgc tcagtcaagg aatacataag agcattacct
     1201 cctgattctg aatgagagga aaatatcaaa tcatccgacc acaaaacact ttgtgtgcct
     1261 aaaccaattc catggttatt gtttctactt cgtaaataac ttacagtttg ccaaagttca
     1321 gaaaggtagc cacctttgaa tccaaatgga tttcggagag gctctcgttc aaaccgggaa
     1381 tcaacaccaa taatttttat atttcctctt acatatttta aagataaagt cttcgcaggt
     1441 tcagatgctg tttccgtttt gttgtttttt tttggtatac agccaggact taacaacgtg
     1501 gcaccgccca ataggcccat gctgtttgtt aagaattgtc ttcgtgatat cataagcctc
     1561 agtcaattgt ttcgaagaaa tttcacagta taaagaaata gtataaaaat aataatttta
     1621 cttaacattc cgcacaacaa ccctcaaaaa acgtaattac ctcatttcag ttctgcttgc
     1681 aaagcatctg ttttagatac attttacata tccaattcat tgtaacggtt tattactgtg
     1741 tcgacctcga aatatgttaa caacctataa atgagattta atgatgttct atgcgtaata
     1801 aaagaagcat aaggccttcg catcctttat gcctgaaaag cataatacga ggcatttttt
     1861 tttgatagac ggcatcggat ttattccttc agagttttta accaataaaa catcttctgc
     1921 cgcataaatg tggaaaatcg attgtaatca ttcggggtgt agagttgaat attatctttc
     1981 gaggaatata atccataaat ttgccctacc tgtccaacgc attttttaat atcatccagg
     2041 tgcgcatctt tatcccgttc tggagcaata agtaaaaccg gacggggagc gatacaacct
     2101 ataatttgat cgaaatcaac aggtatttca ctttcatgcc cgacgaaaaa ccccaacttt
     2161 gggataagcc catgcaaatg agaatacgcc ttaattcctt ctgtcccccg atccagtgtg
     2221 ttcgtacgca tgggagtaaa accggctatg gatacaacac cggcaattct ctcatccagc
     2281 gctgccgata aaagagctac ggtacctcca agagaatatc cagacacgac tattctcgaa
     2341 cgatccacga aatcaaggtt tgaaattgca tcgacagccc ctttcatgtc agcaaccatt
     2401 ttccccattt tcgaccatag gggataacgg tcataaaaat tgactccttc ctccaatcga
     2461 tttccaaaac caatcatatc gaatgcaaaa acaccatatc caagtttggt caaatcctca
     2521 aaaaccgatt gaatctcgtg atcgtaactc atggaagaaa aacctttcga atagtcgtat
     2581 tcatgaaggt aaatcaccac aggaagatct tcacctaaga gcttacccgt ttcatccaca
     2641 ggataataaa gatagccgaa caggttgtca ccaaatccat cgtaaggtgt gatagccatg
     2701 attttcatcg atttggtttc cctgggtctg gagaggaagg atccgaatcg ttcttcacca
     2761 atatctcctt ttgctaggct tcgcggtccc gggtttgtaa cacccgccgg ttcatcgccc
     2821 aacatccatc ggaggttatt ttttatgatt tgtttactat ccttccactt attcctgttg
     2881 tttgcgtata catccaggtc gatagttgct agtccgtgat catggggtgt cacggactga
     2941 ttacttagag cacaccatgt ttcaaaagag taattatgca ataatctgtt tcctggcttt
     3001 ctatcggttc tattaaatac aaaatcaaaa aaatcaatgt aaccttcaat atcgtttgca
     3061 ttcactccat gtaacccgta acgtgacgtt atcgcgatat tatcacccgc attcagaaat
     3121 tgatatactc tcttactgga atgataagct tgctcaattc caaaaatatt acttgccgat
     3181 tcagttgttg ctgtacttat cattaaccct cgtggagcaa ttaatgccat aaatgaattt
     3241 tgatctatag gcaatttatt ttctcttcca acaaagaatc ttaatctggg atgtaaccag
     3301 gccggttgtg cgcaagaaag cagcgcaata tcttcaacat catatttatg ggcattataa
     3361 cgccagggta cttcggcacc cgtgccacca ctgctgggaa tacaagcacc aattctctc
//
LOCUS       DAOSDR010000348         3388 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrimonas sp. isolate AMR_MDS_1826
            NODE_9889_length_3388_cov_3.111311, whole genome shotgun sequence.
ACCESSION   DAOSDR010000348 DAOSDR010000000
VERSION     DAOSDR010000348.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863355
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrimonas sp. (wastewater metagenome)
  ORGANISM  Petrimonas sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Dysgonomonadaceae; Petrimonas.
REFERENCE   1  (bases 1 to 3388)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3388)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:10:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,720
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 35
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3388
                     /organism="Petrimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9889_length_3388_cov_3.111311"
                     /isolate="AMR_MDS_1826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2023866"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1673
                     /locus_tag="PLI30_11650"
     CDS             <1..1673
                     /locus_tag="PLI30_11650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019538965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HOI80242.1"
                     /translation="IKAILNELSKNPNIILFIDEIHTIVGAGGAAGSLDAANMLKPAL
                     ARGEIQCIGATTLDEYRKNIEKDGALERRFQKVIVDATTPEETLQILRNIKERYEEHH
                     NVRYTDEALEACVKLTERYVSDRTFPDKAIDALDESGARVHISNISIPKVIEEMEEEL
                     KVVEQMKNDAVKAQKYELAASYRDKQRQLLLALEAEEQRWQKEIKEKPEIVDEEKIAE
                     VVAMISGVPVQRIAQAEGQRLLEMKNELKAQVIGQDEAVDKIVKAIQRNRVGLKDPNK
                     PIGTFMFLGPTGVGKTHLAKKLAEFLFDSHENLIRVDMSEYMEKFNVSRLVGAPPGYV
                     GYEEGGQLTEKVRRRPYSVVLLDEIEKAHPDVFNILLQILDEGHITDSLGRKIDFKNT
                     ILIMTSNIGTRQLKDFGRGIGFSTNSDVSDTEYSRSVIQKALNKAFAPEFLNRVDDIV
                     MFDQLSKDSIFKIIDLELNGLYKRIKELGYCVELTTEAKEFLADKGYDVQFGARPLKR
                     AIQKYVEDEMAEVILRTGIKEGETVVVDFNKEQQKIEIKIAKHEKIEQ"
     gene            1920..2636
                     /locus_tag="PLI30_11655"
     CDS             1920..2636
                     /locus_tag="PLI30_11655"
                     /inference="COORDINATES: protein motif:HMM:NF013488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family oxidoreductase"
                     /protein_id="HOI80243.1"
                     /translation="MKVEVWTDIMCPYCYIGKIHYEQAMKQFAHADEVELVIKSFRLN
                     PDLPGKGNGYSVVDYLLKTAGLPENKLKQMFAYIEQLAKNAGVRFNLPNAFAANTLDA
                     HRLVKLAAKRNLSSNVLSLISKAYFEDAQDYSDIRILTEIGVKAGLDATEIARLFAGD
                     DFIAEVERDVQEAHQLGIDTVPTFLFERKQAIIGSEPVQVFLDTLNQAYESWKKANTT
                     LGNMEVKKGKSCNADGTCEI"
     gene            2654..3202
                     /locus_tag="PLI30_11660"
     CDS             2654..3202
                     /locus_tag="PLI30_11660"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOI80244.1"
                     /translation="MEFLRIEQSDKSRWNKVWELYESSFPIAERRKMKDHVRACSDPQ
                     FHPVSAWEDGELIGLLFYWEWDMYRYLEYLAIAPGLRGHGFGSQLLRYLRDSDHTIIL
                     EIDPLINELSVRRLQFYERAGFTLTPYRFMHLPYRLEGQPIELLILSYPKMITKEQHN
                     DFLKFLGKRVILYCEGYPFDQR"
BASE COUNT         1024 a          770 c          847 g          747 t
ORIGIN      
        1 ggatcaaagc catactgaac gaactgtcta aaaatccgaa catcattctc tttatcgacg
       61 aaattcacac cattgtaggt gccggaggag ctgccgggtc cctagacgcg gcaaacatgc
      121 tgaaacctgc gctggctcga ggtgagattc agtgtattgg cgctaccacg ctagacgaat
      181 atcgcaagaa cattgaaaaa gacggtgcac tggaacgtcg tttccagaaa gtaatcgttg
      241 acgctaccac ccccgaagag accttgcaaa tcctccggaa cattaaagag cggtacgaag
      301 aacatcacaa cgtccgttat accgacgaag cgttggaggc atgcgtgaag ctgacagaaa
      361 ggtatgtatc cgaccgtact tttcccgata aggccatcga tgccctggat gaatccggcg
      421 cgcgtgttca tatttccaac atctccattc ccaaagtgat tgaagagatg gaggaagagc
      481 tgaaagtggt ggaacagatg aaaaacgacg cggtgaaagc ccagaaatat gaattggcgg
      541 caagctatcg cgacaaacaa cgccagcttt tgctggctct ggaagctgaa gagcaacgct
      601 ggcaaaaaga aataaaggaa aaaccggaaa tcgttgacga agaaaaaatt gctgaagtgg
      661 tggccatgat atcgggcgtg cctgttcagc ggattgccca ggccgaaggc caacgcttgc
      721 tggaaatgaa aaacgagctc aaagcgcagg tcatcggcca ggatgaagcc gtggacaaga
      781 tcgtgaaggc tatccagcgg aaccgcgtgg ggctgaaaga tcccaacaag cctatcggca
      841 cgtttatgtt cctgggacca acaggcgtcg ggaaaacaca cctggcaaaa aagctagcgg
      901 agtttctgtt cgactcgcac gaaaacctta tccgcgtaga catgagcgaa tatatggaga
      961 agttcaacgt ttcccgcctg gtaggagcgc ctcccggata cgtggggtac gaagaaggcg
     1021 gtcagttgac cgaaaaagtg cgtcgccgcc cctactccgt tgtgctgctg gacgaaatag
     1081 aaaaagcgca tcccgatgta ttcaacatct tactgcaaat tctggatgaa gggcatatca
     1141 ccgatagcct gggacgtaaa atcgatttca aaaatacgat cctcatcatg acctccaata
     1201 tcgggacacg acagctaaag gatttcggcc gcggaatcgg gttcagcacc aacagcgatg
     1261 tctccgacac cgaatattcg agaagtgtta tacaaaaagc gctgaacaaa gcgtttgccc
     1321 ccgagttctt gaatcgggta gacgacatcg tgatgtttga ccagttaagc aaagattcta
     1381 tcttcaaaat tatcgacctg gaacttaacg gactatacaa gcgtatcaaa gagttgggtt
     1441 actgtgttga actcactacc gaagccaaag agtttcttgc cgacaaaggt tatgatgtac
     1501 agttcggagc acgtccgcta aaacgtgcca tccagaagta cgtggaagac gaaatggctg
     1561 aagtgatcct gcggacaggc atcaaggaag gcgagaccgt agtggtggat ttcaacaagg
     1621 aacaacaaaa aatcgagata aaaatcgcca agcacgagaa gatagaacaa taatccgcac
     1681 aagagacggg gcagccaaca ggttgtcccg tttttatcaa ttgctgggcg tacaaaccca
     1741 gaaaatggct aaagaaaacg gcctgaaacc agccaaagaa ctccggaaat aaagagcagc
     1801 ctctcaggag gaatcgaata gtcgttcggc aaatttgttg ttttaaataa gtgtattata
     1861 aacaaaaatt cagatcgggt gtttaaaggg ttgtaaaaag caattattaa ataaaacaga
     1921 tgaaagttga agtttggaca gatatcatgt gtccctattg ttacataggc aagattcatt
     1981 acgaacaggc catgaagcaa ttcgcgcacg ccgatgaagt tgaattggtc ataaaatcct
     2041 tccggttaaa cccggatttg cccggcaaag gaaacggcta ctcggtagtg gattatttat
     2101 taaaaaccgc cggacttccg gaaaacaagt taaaacagat gtttgcttac atcgagcaac
     2161 tggcaaaaaa tgcgggagtg aggttcaacc tgccaaacgc tttcgcagcg aatacactcg
     2221 acgcccacag gctggtaaaa ctggctgcaa agagaaacct ctcctcgaac gttttatcgc
     2281 tcatatcgaa agcatacttc gaggatgcac aggattacag cgacatcagg atcctgacgg
     2341 aaattggagt gaaagcaggg cttgacgcaa cagagattgc ccggctcttt gccggagacg
     2401 actttatagc cgaagtggaa cgggatgttc aggaggcgca tcagctggga atagatacgg
     2461 tccctacttt tctgttcgaa cgtaaacagg ccatcatcgg atcggaaccg gtacaggttt
     2521 tcctggatac actcaatcag gcttacgaaa gctggaaaaa ggcaaatacc accctgggca
     2581 atatggaggt aaagaaaggc aaatcgtgca atgcagacgg gacatgtgaa atatagagta
     2641 aaaataatta atcatggagt ttttacgcat agagcaatcg gacaaaagca ggtggaacaa
     2701 ggtctgggaa ttgtatgagt cgagctttcc catcgctgaa aggcgcaaaa tgaaagatca
     2761 cgtccgtgcc tgttccgatc cgcaatttca tccggtatcg gcgtgggaag acggtgaact
     2821 gatagggttg ttgttttatt gggaatggga catgtaccgg tacctggaat atttggccat
     2881 tgcccccgga ttacgcggac acggttttgg gtctcaactg cttcgttatt tgcgcgattc
     2941 cgaccacacc atcattctcg aaatagatcc gttgataaat gagttgtccg taaggcggct
     3001 ccaattttac gaacgtgccg gatttaccct tacgccttac cggtttatgc acctacctta
     3061 ccggttggaa gggcaaccca ttgagctact catcctcagt tatccgaaga tgattaccaa
     3121 ggaacaacat aacgatttcc tgaaatttct gggcaaacgc gttattctgt attgtgaagg
     3181 gtatccgttt gatcagagat aagtcaaccg ccttataggt tatccctgcg ggttcagcac
     3241 cgatttcaat aaccggagcg tttggagcaa gatcttctct tctctttttg aatccgcatt
     3301 catcgggtag acaatcgctt ccgccttgcc atcgtccaga agcctgacat atccgcaaac
     3361 gccccacgac ataatccggc aatcggtc
//
LOCUS       DAOSDR010000349         3386 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrimonas sp. isolate AMR_MDS_1826
            NODE_9898_length_3386_cov_3.973281, whole genome shotgun sequence.
ACCESSION   DAOSDR010000349 DAOSDR010000000
VERSION     DAOSDR010000349.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863355
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrimonas sp. (wastewater metagenome)
  ORGANISM  Petrimonas sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Dysgonomonadaceae; Petrimonas.
REFERENCE   1  (bases 1 to 3386)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3386)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:10:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,720
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 35
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3386
                     /organism="Petrimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9898_length_3386_cov_3.973281"
                     /isolate="AMR_MDS_1826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2023866"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            103..810
                     /locus_tag="PLI30_11665"
     CDS             103..810
                     /locus_tag="PLI30_11665"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070007 - glutamic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PrsW family glutamic-type intramembrane
                     protease"
                     /protein_id="HOI80245.1"
                     /translation="MDLLLISLAPVAIIAGYVWFRDKYEREPLRMLVWALLAGALITL
                     PVVVAEQLISAAGAGLYGMAGVAWQAFAVAAFTEELFKFAALYLLIWRSPEFNDKFDG
                     IVYATFVSLGFAGVENVLYVLEGGWTTGLMRAWTAVPAHAIFGVTMGFYFGMARFYPQ
                     KQQQLKLKAWWMPFLLHGIYDFILMTGIQWLWILFLAFVGFLYLSGWRRMKTLSDQSF
                     YRTDYQLLNRKFNPNNH"
     gene            1036..1686
                     /locus_tag="PLI30_11670"
     CDS             1036..1686
                     /locus_tag="PLI30_11670"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOI80246.1"
                     /translation="MKVILVENALMFRIGIKILLEKGRNLEYIGDVQDGEELLKAIEK
                     KTPDVVIFDGIFTPKKAVPAIKTLVSHHPSIPFLVITELKGFKNLEKYIKLGAKALVF
                     SDATDEELLNAINMTAQGYEYYTVRIVRNISNLLHHYFERRDFEDNFFDGLSKREREV
                     LELFAQGKSYKEISVLLNISIRTVETHKKNIMSKLKLQSTADIVKYALKYNLLHFQ"
     gene            1967..>3386
                     /locus_tag="PLI30_11675"
     CDS             1967..>3386
                     /locus_tag="PLI30_11675"
                     /inference="COORDINATES: protein motif:HMM:NF039534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprB repeat-containing protein"
                     /protein_id="HOI80247.1"
                     /translation="MKKIYRSFRRTAQIFSLFLAVMLVSFMSTAQTITIDGNPSDWPA
                     VLQNTPGVVAWSFVHDANSTNDDQFTTGSQDPDLISEWGWVLGNTNDKGDISNVGAVV
                     IGNYLYFFGDRTAINGDAQIGFWFFLGGVAKSGTGASNSPFSNEHVYGDLLLLSNFTN
                     GGGNVNLRVYMWVGSGGSDGSLDFLGTTTDAAVNSADYPVPVYPNWSYTPKSGTGYVT
                     GSFYEGKIDLGGLTGGICFSSFLFETRNSQSVNASLQDFSGGSFGLKPLPPVTVSDSR
                     CGAGIVNLTASAPAGMTIKWYDDEALTHQVATGTSYSPNLTVTTTYWVTVTNATNCTS
                     DPSTVVGTIYPTPSASAVATNPLCYGGTGSIDLTVTGGTPPYTFLWSNGAIIEDPSGL
                     AAGTYNVTVTDAHQCTTTASATISIPSDIALSLNKTDVLCFGSSDGTITATFSGGTPP
                     YMARIDGGSYAAATSPKAFTGLA"
BASE COUNT          827 a          793 c          848 g          918 t
ORIGIN      
        1 aaagccgaac ccaaaatgac gctggtcatg aagagactgg tggagtcgtt gtaacctcgc
       61 tgactttacg ggatccaatt tttgtcgttt taccccattg tcatggattt gctcctgatc
      121 tccctggcgc cggtcgccat cattgccggc tacgtctggt ttcgtgacaa atatgaacgc
      181 gagccgctcc gtatgctggt gtgggcgttg cttgccggcg ccctgatcac ccttccggtg
      241 gtggtggccg aacagctgat ttcggctgcc ggcgccggct tgtacggaat ggccggcgtc
      301 gcgtggcagg cttttgcggt ggctgctttc accgaggagc tgtttaaatt tgcagccctt
      361 tacctgctca tctggcgaag ccctgaattc aatgacaaat tcgacggcat tgtgtatgcc
      421 acctttgttt ccctcggatt tgccggggtc gaaaatgtgc tttacgtcct cgaaggcggc
      481 tggactaccg gcctgatgcg tgcctggact gccgttccgg cgcacgctat ttttggcgtt
      541 accatggggt tttatttcgg gatggcccgg ttttacccgc aaaaacagca acagcttaaa
      601 ttgaaggcgt ggtggatgcc tttcctcctg cacggaattt atgattttat cctgatgacc
      661 gggatacagt ggctgtggat attgttcctt gcatttgtcg gttttctcta cctctcgggg
      721 tggcgccgca tgaaaacgtt gtccgatcag tcgttctacc gtaccgacta ccaactgcta
      781 aatcgaaaat tcaatcccaa taaccattga tttgatagcc tgaaaaaaac agcttcccct
      841 ttttattgaa gccttctgct aagatcatat gttactattt ttatcgtgca aacgatttct
      901 ttatcaggcg aataatggta aaaatatctc cgagatatct tgttgttgaa tgggattttt
      961 ttcgtaaatt tctatttagt aattagttaa aggatatttg ttgagggctt caagggggat
     1021 caaatgggac tgtgtgtgaa ggttatactg gttgaaaatg ccctgatgtt tcggataggg
     1081 attaaaatac ttctggaaaa gggcaggaac ctggaatata tcggcgacgt tcaggatggg
     1141 gaagaacttt tgaaagcgat tgaaaaaaaa acgcctgacg tggtcatctt cgacggaatt
     1201 ttcaccccga aaaaagctgt tcccgccatt aaaacattgg tcagccatca cccttcgatc
     1261 ccctttctgg taatcaccga actcaaaggg tttaaaaatc tggaaaaata cattaagctt
     1321 ggtgccaagg cgttggtttt cagcgatgct accgatgagg agctccttaa cgccatcaat
     1381 atgacggcac agggatatga atattatacc gttcgtattg tccggaatat cagcaatttg
     1441 cttcatcact attttgagcg gagagacttc gaggacaact ttttcgacgg attgtcgaaa
     1501 cgtgaaagag aggtgctgga gctatttgca cagggaaaaa gttacaagga gatttctgtg
     1561 ctgctgaata tcagtattcg aacggtggag acccataaaa agaatatcat gtcgaaactg
     1621 aaactgcaat ccactgcgga cattgtaaaa tacgccctca agtataatct tcttcatttt
     1681 cagtaggatg tgtgggctta ttaataatta ttcttaattc cgggattgca aaaatcagga
     1741 attctacgga ttgtgtacgg ccctgtttcg tagtaacttt tcattcctgt ttggaaaaca
     1801 aaaaaaagga aacagagaag tgacggactt gaattttcac cccggaaatt taagttgaga
     1861 accaatcatg agttacggga gaaaggcagt taccgtagag gaagattttt aaaggtgaga
     1921 tgaagatctt gtaaatgatg tttttgaatt tcaaaatttc atgtttatga aaaaaattta
     1981 ccggagcttc agaagaactg ctcagatttt ttccctcttt ttggcagtaa tgctggttag
     2041 tttcatgtca acggcccaaa cgatcacaat cgacggaaat ccttccgatt ggcctgcagt
     2101 gttacagaac acgccgggcg tggttgcgtg gagttttgta catgacgcca acagtaccaa
     2161 tgatgaccag tttaccacag gttcgcaaga ccccgattta atcagcgagt ggggatgggt
     2221 attggggaac accaacgaca agggggatat ttccaacgta ggggctgttg taatcggcaa
     2281 ttacctctat ttcttcggcg accggaccgc catcaacggg gatgcccaga ttggcttctg
     2341 gtttttcctg ggcggcgtag ccaaatccgg gacgggcgct tcaaacagcc cgttcagcaa
     2401 tgaacatgtc tatggcgact tgctgctgtt gagtaacttt acaaatggcg gcggaaatgt
     2461 aaacctgcgg gtttacatgt gggtcggctc cggcgggagc gacggctcgc tggacttttt
     2521 gggaactaca accgatgctg ccgtgaattc cgctgactat cctgtaccgg tttatccgaa
     2581 ctggagttat acgcctaaaa gtgggaccgg atatgtaacc ggctccttct atgaaggcaa
     2641 gattgatctt ggcgggctga caggaggcat ctgcttctcc agctttttgt ttgaaaccag
     2701 aaactcccag tccgtaaatg cctccctgca ggatttttcc ggagggtcat tcgggttgaa
     2761 acctctcccg ccggtaaccg tttccgactc ccgttgcggc gccggaattg ttaatctgac
     2821 ggcttccgca cccgcaggca tgaccataaa atggtacgac gatgaagcgc tgacccatca
     2881 ggttgccacc gggaccagtt acagtcccaa tctcaccgtt accaccacct attgggttac
     2941 ggtcaccaat gcgacaaatt gtaccagcga tccttcaaca gtggttggaa cgatctatcc
     3001 gacgccttct gcctctgcgg tagcgaccaa cccgctttgt tacggtggta cgggaagcat
     3061 cgaccttacc gtgaccggcg gtacgccgcc ttataccttc ctttggtcaa acggagcaat
     3121 cattgaagat cccagcggac tggcagccgg aacgtacaat gtaaccgtta ccgatgctca
     3181 ccagtgtacc actactgctt cggcaaccat ttctattcct tcggatattg cgctgagtct
     3241 caataaaacc gatgtcttat gttttggcag cagcgatggt acgattacgg ccaccttcag
     3301 cgggggcact cctccgtaca tggccaggat cgacggcggc agctatgccg cagccacttc
     3361 gccgaaggcc tttaccggcc tggctg
//
LOCUS       DAOSDR010000350         3377 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrimonas sp. isolate AMR_MDS_1826
            NODE_9924_length_3377_cov_3.604154, whole genome shotgun sequence.
ACCESSION   DAOSDR010000350 DAOSDR010000000
VERSION     DAOSDR010000350.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863355
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrimonas sp. (wastewater metagenome)
  ORGANISM  Petrimonas sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Dysgonomonadaceae; Petrimonas.
REFERENCE   1  (bases 1 to 3377)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3377)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:10:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,720
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 35
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3377
                     /organism="Petrimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9924_length_3377_cov_3.604154"
                     /isolate="AMR_MDS_1826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2023866"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            61..423
                     /locus_tag="PLI30_11680"
     CDS             61..423
                     /locus_tag="PLI30_11680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019539495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HOI80248.1"
                     /translation="MAQHNELGKRGEEEAVRYLKSKDYKIVCRNWRFYGYEIDIVAEN
                     EEFIVFVEVKTRTSVQWGNPVGFVNKLRMRRMLDAADHYLIEMDIDKPARFDIIGLVW
                     NGREFDLEHIDDAFLPFL"
     gene            465..827
                     /locus_tag="PLI30_11685"
     CDS             465..827
                     /locus_tag="PLI30_11685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014224095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HOI80249.1"
                     /translation="MNIEELYDYCLSIPGAEVTTPFDDVTLVMKVCDKMFALIPLDAD
                     RLSVALKCEPEKAVRLREEYRCVEPAFHMNKTYWNTIYITGEMPDEELKIWIRHSVEE
                     VIKKLPKIKQKEYYGSVE"
     gene            811..1560
                     /locus_tag="PLI30_11690"
     CDS             811..1560
                     /locus_tag="PLI30_11690"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019539497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HOI80250.1"
                     /translation="MDQLSEDRKIVRLEETESTNSYLKELLRTEHLEEGSVVVADFQT
                     AGRGQMGNSWYSTKGDNLLFSLLIYPIEIPANEQFIISRIISLAIKNTLDQFTDDIRI
                     KWPNDIYWKDRKIAGILIENSLQGKIIENSIIGIGLNLNQQVFPPELPNPVSLRQITA
                     TEQDKDYILDLLMKEFFLLYRGLQRGEKQVIEDEYMLDLYRVNGYYWFEDANGKFQAK
                     IDNVLPSGHLVLKTLETNEERMYAFKEIAFI"
     gene            1572..2279
                     /gene="pyrH"
                     /locus_tag="PLI30_11695"
     CDS             1572..2279
                     /gene="pyrH"
                     /locus_tag="PLI30_11695"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006843276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HOI80251.1"
                     /translation="MKYNRILLKLSGESLTGEKGYGIDEVRLNDYARQIKEAAQMGVQ
                     VGIVIGGGNIFRGLSGSQKGFDRVKGDQMGMLATVINSLALSSALTAIGQKNRVYTSI
                     RMEPVGELYSKWKAIESMQNGEVAILSGGTGNPFFTTDTASALRGIEIEADAMFKGTR
                     VDGVYTADPEKDPSATKFDTISFDEVYDRGLRVMDLTATTMCKQNNLPIVVFDMDTYG
                     NLKKVLSGDNIGTLVHV"
     gene            2336..2893
                     /gene="frr"
                     /locus_tag="PLI30_11700"
     CDS             2336..2893
                     /gene="frr"
                     /locus_tag="PLI30_11700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019539500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HOI80252.1"
                     /translation="MDISTIKKDAEEKMQMTLEFLEETFARIRAGRANVRILDGVRVE
                     YYGSHVPLSNVSTVSTPDAKTILVQPWEKNMLKVVEKAIMDSDVGITPENNGEVIRLG
                     IPPLTEERRRQLVKQTKQESEDAKISIRNSRREGIDEVKKAVKDGLPEDMGKDVENEL
                     QKLHDKFIKRIDDKFAEKEKEILTV"
     gene            2900..>3377
                     /gene="rsgA"
                     /locus_tag="PLI30_11705"
     CDS             2900..>3377
                     /gene="rsgA"
                     /locus_tag="PLI30_11705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013617386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase RsgA"
                     /protein_id="HOI80253.1"
                     /translation="MSEEGSKRGLVIRNTGNNYLVRTDEGTDMSCLAKGNFRLKGIRS
                     TSPVVVGDRVKMDINPDGTAYITEIEDRKNYIVRKASNLSKHSHILAANIDLALLCVT
                     VRFPETTTVFIDRFLVTAEAYSVPVVLVFNKTDIYDSDDREYVDGLVHLYSTVGYTC"
BASE COUNT         1035 a          611 c          859 g          872 t
ORIGIN      
        1 tgcataaatt tgtatttcta agcatttgtt ggttttataa ccggccgata tttcaaaaaa
       61 atggcacaac acaatgagtt aggcaaaaga ggcgaagagg aggccgtaag atacctgaaa
      121 tcgaaagatt ataaaattgt ttgtcggaac tggagatttt acggctacga gattgatatt
      181 gttgcggaga acgaagaatt tattgtcttt gtcgaggtga aaaccagaac gtctgttcaa
      241 tgggggaatc cggtgggttt tgtgaataag ctccgaatgc gtagaatgtt ggatgctgcc
      301 gatcattatc tgattgaaat ggatatcgac aagcctgccc ggttcgatat tatcggtctg
      361 gtctggaatg gaagagaatt tgacctggag catatcgatg atgctttttt gcccttcctg
      421 tgagcattgg ttatttacct tacgcgatta tcaatcagga atatatgaat atagaagagc
      481 tttatgatta ctgtctttcc attccgggtg cggaagtaac aacgccgttt gacgatgtta
      541 cattggtgat gaaagtgtgt gacaagatgt ttgcccttat cccgttggat gctgacagat
      601 tgtctgtcgc gctcaaatgt gaaccggaaa aagcggtgcg gttgcgtgaa gaatacagat
      661 gtgtggagcc tgcttttcat atgaacaaaa cgtattggaa cacaatttat ataactggag
      721 aaatgccgga tgaggaatta aaaatatgga tcaggcactc ggtggaagaa gtcattaaga
      781 aattacccaa aataaagcag aaagagtact atggatcagt tgagtgaaga cagaaaaatt
      841 gtgcggttgg aggaaacgga atcgacaaat tcctacctga aagagttatt aagaacagaa
      901 cacttggaag aaggttctgt tgtggtggct gattttcaaa cggcaggccg tggacagatg
      961 ggcaacagct ggtattcgac aaaaggagat aatttgcttt tcagtctgtt gatttatccg
     1021 atagagattc ctgctaacga gcagtttatt atctcccgga taatttcact ggccataaaa
     1081 aataccctgg atcagtttac ggatgatatc cggatcaaat ggcccaacga tatttactgg
     1141 aaagaccgga aaattgccgg tattctcata gaaaacagtt tgcaggggaa aataattgaa
     1201 aactccatca ttggaatcgg gttgaacctc aaccagcagg tgtttccgcc ggaattgccc
     1261 aatccggtat cgttgaggca aatcactgca accgaacagg ataaagacta catcctggat
     1321 cttttgatga aagagttttt tctgttatac agggggttgc agcgcggaga aaaacaagtt
     1381 attgaagatg aatacatgct tgatttgtac cgggtaaacg gttattactg gtttgaagat
     1441 gcaaacggca agtttcaggc aaaaatcgat aacgtgcttc cgtccggtca tcttgtattg
     1501 aagacgcttg agaccaatga ggagcgaatg tatgcgttta aggaaatagc ttttatatag
     1561 aacagatata gatgaaatac aacagaattt tactgaaact cagcggtgag tcgctgacag
     1621 gagaaaaagg gtacgggata gatgaagtcc ggttgaatga ctatgcccga caaataaaag
     1681 aagctgcaca aatgggagtg caagtcggaa ttgttatcgg tggtggaaat attttccggg
     1741 gcttgagcgg ctcgcaaaaa gggttcgacc gggtaaaagg tgaccaaatg ggtatgcttg
     1801 ccaccgttat caatagcctg gcattgagtt ctgcattgac ggccattggt cagaaaaaca
     1861 gggtttatac ctccattcgt atggaacctg tcggcgagct ttactccaaa tggaaagcga
     1921 ttgagtccat gcaaaatggt gaagtggcta tcctttccgg aggaacggga aacccgttct
     1981 ttaccaccga caccgcttct gctctgcgtg gaatagagat tgaggctgat gccatgttta
     2041 aaggcacccg tgttgacggt gtttatactg ctgaccccga aaaagatccg tcggcaacca
     2101 agttcgatac gatctcattt gatgaagttt acgaccgggg cttgcgggta atggatctta
     2161 cggcgacgac aatgtgcaag caaaataacc tgcccattgt tgttttcgat atggacacat
     2221 acggaaacct gaagaaggtc ctttccgggg ataatattgg aaccctggtg cacgtgtgaa
     2281 agatttattc aaatattttc actatttttg ctaacataac atcaaacaaa aagatatgga
     2341 tatttcaaca attaaaaaag atgccgagga aaagatgcag atgaccttgg aatttctgga
     2401 agaaactttc gcacgaattc gtgcagggag agctaatgtg cgtattcttg acggggtgag
     2461 agtagaatat tatggcagtc acgttccgtt gtcgaatgta tcaacagtct ctactcctga
     2521 tgccaaaact attctggttc aaccgtggga aaaaaatatg ctgaaagtag ttgaaaaagc
     2581 gattatggat tctgacgtgg gaattacccc cgagaacaac ggtgaagtta tccgcctggg
     2641 aatcccgccg cttaccgaag aacgccgcag gcagcttgtg aagcagacca agcaggaatc
     2701 ggaggatgcc aagatcagca ttcggaactc ccgccgcgaa gggattgatg aggtgaaaaa
     2761 ggctgttaaa gatggacttc ccgaggatat gggaaaagac gttgaaaatg agcttcaaaa
     2821 acttcacgat aaattcatca aaagaattga tgataagttc gccgaaaagg aaaaagaaat
     2881 acttaccgta taaatagcga tgagcgagga gggaagtaag aggggactgg taatcagaaa
     2941 tacgggcaat aactatctgg taagaacgga cgaagggacg gacatgtcgt gtctggcaaa
     3001 agggaatttc cgtcttaagg gtatccggag cacgagccct gtggtagtgg gagatagggt
     3061 gaaaatggat ataaaccccg acgggactgc atatattacc gagattgaag accgtaaaaa
     3121 ctatattgtc cgcaaggcgt ccaacctttc caaacactcg catattcttg ccgccaacat
     3181 tgatctggct cttctttgtg ttactgtaag gtttccggaa acaaccacag tatttatcga
     3241 ccggtttttg gttaccgcag aggcatacag tgttcctgtt gttcttgttt tcaacaaaac
     3301 ggatatctac gacagcgatg acagggaata cgtggatggg ttagttcact tgtattcaac
     3361 tgtcgggtat acatgca
//
LOCUS       DAOSDR010000351         3371 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrimonas sp. isolate AMR_MDS_1826
            NODE_9946_length_3371_cov_2.870929, whole genome shotgun sequence.
ACCESSION   DAOSDR010000351 DAOSDR010000000
VERSION     DAOSDR010000351.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863355
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrimonas sp. (wastewater metagenome)
  ORGANISM  Petrimonas sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Dysgonomonadaceae; Petrimonas.
REFERENCE   1  (bases 1 to 3371)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3371)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:10:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,720
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 35
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3371
                     /organism="Petrimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9946_length_3371_cov_2.870929"
                     /isolate="AMR_MDS_1826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2023866"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1530)
                     /locus_tag="PLI30_11710"
     CDS             complement(<1..1530)
                     /locus_tag="PLI30_11710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008776875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HOI80254.1"
                     /translation="MKLTIYKSIQLILLIVSAFGIISCTDFLQEDPKGRLATNAFFTN
                     KGDLDASLNALYAIVADAQYANHHTGTNFTVGDDISTHPASNKQPLREHDQFNVSDNN
                     PWMPYLWERRWRIVKAANFVINNVERTPDVSQAEIASAIAQAKYWRAYAYFYIVTTWG
                     PVPIMLDEEVNYESELNSEQQIYDLIVEDLKYAETNLPIMHTKEPYARGGVNIAVGQA
                     AAKATLSYVYMCMAGWPLNKREYYQLAADKAKEVIDAVNNGTYNYKLLDEYWKVYSWE
                     YNNSNPEVLLGIYYNRDRTANMSTVTDFLQDMKQAGWGDMNGEIKFWKDFPDGPRKEA
                     TYFPKIMLADGKLYDWWYDTEPASRAVVAPCFIKSAEGAVRGTEFDYADPSAISYAGE
                     KQHQVIRLAEVYCIYAEAVGRSGQINALAVDVLNKVRNRADGTLSNIYSTSMTPNELA
                     EAGYNEHGWEIAGYCWGGLTSRARNMFRMYRYKDHFEFRKLNPLIEVAPGIYRKEAVP
                     VT"
     gene            complement(1566..>3371)
                     /locus_tag="PLI30_11715"
     CDS             complement(1566..>3371)
                     /locus_tag="PLI30_11715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009001008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0071702 - organic substance transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SusC/RagA family TonB-linked outer membrane
                     protein"
                     /protein_id="HOI80255.1"
                     /translation="TPAMEMRNEVTGVYNMDPYNSVDANPYAAKMVNVSDNFRYHINA
                     NTTLLFKLYPELTLSVQGGYNYQHNPNYSFTSSLYRPAQIPTASNGSGMTNYWQNTNN
                     LTYQKNFGNHNLIATAVWEISNNQYSGLWGSGSNLKNESVGYWNLANGGTLSTGNDYS
                     EESIASGLGRVMYNYNNRYFVTGTFRADGSSKFQGDNKWGYFPSASVAWDAAREEFMK
                     DQNIFQQMKLRASYGITGNQAIDRYSTLGMLSATDYGFGTSSPFAGYWGYTFATPDVS
                     WEKTYQYDLGADFSILQGKVNVTMDWFLKQSKDLLFQKAVPMYNGGGSFWVNQGEVKN
                     SGFELGITAYPLREGGAINWETSFNGTYIKNEIVDLAGQDFILDATYSNYGGSMQILK
                     PGYPYGSFYLYQWEGFNDEGANLYRAADGTLTTSPTADDQYVMGQASPKFTLGWNNMI
                     SWKNWTINVFINGAFGADRLNLTRYALSSMIGMYRFISLRDSYFKGWDKVSNKADAMF
                     PSHTNSDNKYYGNSTYWLEDASFIKLKNISVGYTLPKEITRIFDLQFSVSAQNIYTLT
                     KYKGMDPEAYNAYNGLDLGAYPVPRTVTFGAKLTF"
BASE COUNT          945 a          741 c          617 g         1068 t
ORIGIN      
        1 ggttacaggt actgcttctt ttcgatatat gcccggagct acttcgatca atggatttag
       61 tttcctgaat tcgaaatgat ctttgtagcg atacatacgg aacatattgc gagcacgtga
      121 ggttaatcca ccccaacaat aaccagcgat ttcccatccg tgttcattgt atccggcttc
      181 cgcaagttca ttaggagtca tgctggttga atagatatta gataatgtgc catctgccct
      241 gttacgcacc ttgtttaata catccacggc taaggcatta atctgtcctg atcgacctac
      301 agcctcagca tatatgcaat atacttctgc aagtctgatt acctgatgtt gcttttctcc
      361 cgcgtagctg atagcacttg gatcggcata atcaaactct gtgcctctaa ccgctccttc
      421 tgcagattta ataaaacatg gagcaacaac ggctcttgac gcaggctcag tatcatacca
      481 ccagtcatat agctttccgt cggctaacat gatttttggg aagtaagtgg cttctttacg
      541 tggtccatcg ggaaagtctt tccaaaactt aatttcaccg ttcatatcac cccaaccggc
      601 ttgtttcata tcttgcaaaa agtccgttac agttgacatg ttagccgttc tatccctatt
      661 gtaataaatt ccgagcagta cttccggatt gctattgttg tactcccatg aatacacctt
      721 ccaatattcg tctaacaact tataattata agtgccatta tttacagcat caatgacttc
      781 tttcgctttg tcggcagcca attgatagta ttccctttta tttaaaggcc agccagccat
      841 acacatataa acatatgaaa gtgttgcttt agcggcggcc tgacctacgg caatatttac
      901 accgcctctg gcgtagggct cttttgtatg catgatagga agattggttt cggcatactt
      961 taaatcttca actatcagat cataaatctg ttgttctgaa ttaagttctg actcataatt
     1021 tacctcttcg tccaacataa taggcaccgg tccccatgtg gttacaatgt aaaaatatgc
     1081 ataagcacgc caatatttcg cttgtgcgat agcagaagcg atttctgctt gcgaaacatc
     1141 aggagttctt tctacgttat taatcacgaa attagcagct tttactattc tccaacgtcg
     1201 ctcccataaa tatggcatcc agggattgtt atcagatacg ttaaactggt cgtgttcacg
     1261 caggggctgt ttattacttg ccggatgcgt ggaaatatca tcaccaacag taaaattagt
     1321 tcctgtatga tgatttgcat actgcgcatc agctacaatg gcgtacaaag cgttcaatga
     1381 agcatccaaa tcacccttgt tcgtgaagaa tgcattcgtg gccaatcgcc cttttgggtc
     1441 ttcttgtaag aaatcagtac aagaaattat tccgaatgct gaaactatca ataatatgag
     1501 ttgaattgat ttatatatag ttaatttcat catatgtaat atttaaaata taattattcc
     1561 tgccgttaaa aagttagttt tgcaccaaat gttactgtac gtggtactgg ataggcacct
     1621 aaatccaatc cgttataagc attatatgcc tcaggatcca ttcctttata ttttgttagt
     1681 gtatatatat tttgtgcact aaccgaaaat tgaagatcaa aaatgcgtgt aatttctttc
     1741 ggcaaagtat atccgacact gatattcttt aatttaataa aggaagcgtc ttccaaccag
     1801 taagtagaat tgccataata tttattatca gaattggtat gacttgggaa catagcatct
     1861 gccttgtttg aaactttatc ccatccttta aaataggaat ctcttaggga gataaatcta
     1921 tacataccga tcattgaaga aagggcatag cgagttaagt ttaatcgatc cgcaccaaag
     1981 gctccattga taaaaacgtt tatggtccag tttttccagc taatcatgtt attccatccc
     2041 agcgtaaatt tcggactggc ttgtcccatt acatactgat catcggccgt gggactggtt
     2101 gtcaaagtgc catcggccgc cctgtaaagg ttagctcctt catcattgaa accttcccat
     2161 tgatacaaat aaaacgaacc gtatggatac cctggtttca agatctgcat agatccgcca
     2221 taattgctat atgttgcatc taatataaaa tcctggcctg ccaagtccac aatttcattc
     2281 tttatgtagg tgccattaaa actggtttcc caatttatag ctcctccctc tctcagcggg
     2341 tacgcagtga taccaagttc aaagccagag ttcttcacct ctccctgatt aacccagaaa
     2401 ctaccccccc cgttatacat aggcacagcc ttttggaaaa gaagatcttt tgattgtttt
     2461 aaaaaccagt ccattgttac atttactttc ccctgtaaga tactgaaatc cgcacctaag
     2521 tcatattggt acgttttttc ccaagagaca tcaggcgttg caaacgtgta tccccaataa
     2581 ccggcaaacg gagatgatgt tccaaatccg tagtcagtag ctgaaagcat tcccagggta
     2641 ctgtatcgat caattgcctg attacccgtg ataccataac ttgctcttaa tttcatttgt
     2701 tggaatatat tctgatcttt catgaattct tctcttgcag catcccaggc taccgaagcc
     2761 gatggaaagt aaccccattt gttgtctccc tggaattttg aagatccgtc tgccctgaat
     2821 gtgccggtta caaaataacg gttattatag ttatacataa cacgtccaag acctgaagca
     2881 atagattctt ccgaatagtc attacctgtg gatagtgtgc caccattagc aaggttccag
     2941 tatccgacac tttcgttttt caaatttgaa cctgagcccc ataaaccgga atactgatta
     3001 ttactgatct cccaaacggc agttgcgatg agattatgat ttccgaaatt tttctgataa
     3061 gtcaaattat ttgtattttg ccaatagtta gtcatccccg atccatttga ggcagttgga
     3121 atttgtgccg gacgatacaa agacgaagtg aaggagtaat ttgggttatg ttggtaatta
     3181 tatcctccct gtaccgatag cgtaagttct gggtaaagct tgaacaataa cgtggtattt
     3241 gcatttatgt gatatcggaa attatcgcta acgtttacca ttttagcggc ataaggattg
     3301 gcatctaccg agttatacgg gtccatatta tatacgcccg ttacttcgtt tctcatttcc
     3361 atagcaggtg t
//
LOCUS       DAOSDR010000352         3331 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrimonas sp. isolate AMR_MDS_1826
            NODE_10116_length_3331_cov_2.750916, whole genome shotgun sequence.
ACCESSION   DAOSDR010000352 DAOSDR010000000
VERSION     DAOSDR010000352.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863355
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrimonas sp. (wastewater metagenome)
  ORGANISM  Petrimonas sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Dysgonomonadaceae; Petrimonas.
REFERENCE   1  (bases 1 to 3331)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3331)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:10:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,720
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 35
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3331
                     /organism="Petrimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10116_length_3331_cov_2.750916"
                     /isolate="AMR_MDS_1826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2023866"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1002)
                     /locus_tag="PLI30_11720"
     CDS             complement(<1..1002)
                     /locus_tag="PLI30_11720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019539637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HOI80256.1"
                     /translation="MNNPELETQNSKLRTDNSFHESRDSAMDKRLPKRSFFQKYKYYL
                     LAGAAFVAFLVFVIVSVSGGRKLRVDDEKIVIANVAEAPFLDYVDAEGIVQPIQTIKL
                     NALESGMVQEVVAEEGAMLKKGDVILVLQNPELERIIEEQQAEWEKQRILYEEKKLEM
                     EQKTILLKQQTLQARYELSRLQKDFALGEEEFKMGVKSKAQLNVQREEFTYKTQSTAL
                     QLDGLRQDSAATHLRRTLMDNDLERVRKTTSHARGRMDNLIVRAPMDGQLSFLNVTLG
                     LRVGQSESIGEIKVMDNFKIHTRLSEYYIDRVQVGLPASVTYQGKKYPLRVSKVVPEV
                     "
     gene            1178..2317
                     /locus_tag="PLI30_11725"
     CDS             1178..2317
                     /locus_tag="PLI30_11725"
                     /inference="COORDINATES: protein motif:HMM:NF025019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4143 domain-containing protein"
                     /protein_id="HOI80257.1"
                     /translation="MLSRKITIQDIENESIFLWGARQTGKSTLLKMLFPDKRIIDLLK
                     SDEFERYNRRPALLREELSLLPVNELVIIDEIQKIPALLDEVHWLISNHNLRFILSGS
                     SARKLRRSGVNLLGGRAIRKHLYPFVSAEIPDFDLVKACNNGMLPRHYLVENATNRLH
                     AYVGDYLQQEIKAEALTRNLNTFSRFMEIAALSNGEILNYNNIASECGVSAPTVKEYF
                     SILEETLIGYTIPAFTRNVKRRVIQSPKFYYFDVGIANFLLKRTEINPGSPEFGHAFE
                     HLIMQELIAYLGYFKPQLSLSYWRTTSGYEVDAIIGNAETAIEIKSSDEVQSHHTKGL
                     KAFSEEFPDCRLIIVSLDKNPRRMNNVEIYPVLQFLSMLWSGDFF"
     gene            complement(2386..>3331)
                     /locus_tag="PLI30_11730"
     CDS             complement(2386..>3331)
                     /locus_tag="PLI30_11730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019539636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HOI80258.1"
                     /translation="ATAQSNLRSANYNYANADTLYTYAKGRYEIGTITENEMLQLEIN
                     YLSEQTNRLNARIEMDDRIQDLRSFLGITDNVEIVVQVSEAVPEFMVPVNEALELARQ
                     NSPDMELLALQKLQSESAVAYAKASRGFKADLYMQFGLSQTDRSFANVYHNPLNQQLV
                     SLGIRIPILDWGVGRGRVEVAKSNLEKVKIDIAQARTDFDSNVIKLVKQFNLQADKVA
                     IAQKTSERAARRNDVAYRLYLLGKSTVLDLNAATAEKDRSQRDYIRELQNYWSLYYGL
                     RSITGWDFEKNSPILNVSVQKIEYRCQKTEDNFYSLIK"
BASE COUNT          808 a          772 c          756 g          995 t
ORIGIN      
        1 cacttcggga acgactttcg acacgcgtag cggatatttc tttccctgat aggttaccga
       61 tgcaggcaat cccacctgta cgcggtcgat gtaatactcg ctcaatcggg tatgtatctt
      121 gaaattgtcc atcactttta tttcgccaat gctttccgat tgtcccaccc gcaggccgag
      181 cgtaacgttg aggaaactca attgtccgtc catcggggcg cgcacaatta aattatccat
      241 tcgtccgcgg gcgtgtgagg tggtttttcg tacccgctcg aggtcattgt ccatcagcgt
      301 tcggcgcagg tgtgtggcgg cgctgtcttg tcttagcccg tctaattgca gggcggtgct
      361 ttgggttttg taggtaaatt cttcgcgttg cacattgagt tgcgccttgc tttttacacc
      421 catcttaaat tcttcttcgc ccagcgcgaa gtctttttgc aaacggctca gttcgtatcg
      481 cgcctgaagt gtttgctgct tgagcaggat ggttttctgc tccatttcca gctttttttc
      541 ttcgtaaaga atgcgctgtt tttcccattc ggcttgctgc tcttcgatga tgcgctctaa
      601 ttcgggattt tgcagcacga gaatcacgtc gccctttttc agcatcgccc cctcttcggc
      661 aaccacttcc tgcaccattc cgctttccag cgcgttgagc ttgatggttt ggatgggctg
      721 cacgatgcct tcggcatcca cgtaatcgag aaacggcgct tccgccacgt tggcaatcac
      781 gattttttcg tcgtccacgc gcagttttcg tccgcccgac acgcttacga taacgaatac
      841 gaggaacgcc acaaacgccg ctccggccag caggtagtat ttgtattttt ggaaaaacga
      901 gcgtttgggc aagcgtttgt ccatagcgct gtcgcgggat tcgtggaaag agttatcggt
      961 tctaagtttt gagttctgag tttcgagttc cgggttgttc atttttatgt tggccattgg
     1021 ccattggcgt taaaagtcta ttatctgaaa tctggatgaa aaaacaaagt tgtactttag
     1081 atattcatga aaaaacaaag ctatacttta gatattcatg aaaaataatg tatctttgta
     1141 acggttttac cttattattc acaaaaaaca aatcagaatg ttatcacgaa aaataacaat
     1201 tcaggatata gagaacgaaa gcatttttct ttggggtgcg cggcaaaccg gtaagagtac
     1261 gctgctgaaa atgctgtttc ccgacaaacg aatcatcgat ttgttgaaat cggatgaatt
     1321 tgaacgttat aaccgccgtc cggcactgtt acgcgaagaa ctaagcctgt tgcccgtaaa
     1381 cgagttggtt atcatcgacg aaattcagaa aatccctgct ttacttgacg aagtgcattg
     1441 gttgataagc aatcataatc ttcgttttat cctgagcggc tccagtgcac gcaagttacg
     1501 ccgaagcggt gtgaatttgc ttggaggcag agctattcga aagcacttgt atccgtttgt
     1561 cagcgccgaa attcccgatt tcgaccttgt aaaagcctgc aataacggta tgctgccgcg
     1621 ccactattta gtggaaaacg ccacaaaccg gcttcacgct tacgtgggcg attatttgca
     1681 acaggaaata aaagccgaag cacttacgcg aaacctgaat actttttctc ggtttatgga
     1741 gattgcggct ttgagcaatg gtgaaattct caattacaac aacattgctt cggaatgcgg
     1801 cgtgagtgca cctacagtga aagagtattt ttctattttg gaagaaacgc tgataggtta
     1861 taccattccc gcttttaccc gaaacgtgaa acgtcgtgtt atccaatctc ccaagtttta
     1921 ctattttgat gtaggcattg ctaattttct tctcaaaaga actgagataa accccggttc
     1981 acccgaattt ggacatgctt tcgaacattt aatcatgcaa gaacttatag cctaccttgg
     2041 ttatttcaag cctcaactca gcttgtccta ttggcgcaca acatcgggtt acgaagtgga
     2101 cgccattatc ggaaatgccg aaacagccat tgaaataaaa tcatccgacg aagtgcaatc
     2161 gcatcacaca aagggattaa aggcattttc ggaagaattt cccgattgcc ggctgattat
     2221 tgtttcgttg gataaaaatc cgcgaaggat gaacaatgtg gagatttatc ccgtgttaca
     2281 attcttgtcg atgctttgga gcggcgattt cttttgatct gatcaatttt cgcttatatt
     2341 attatttcaa tttttgaagt agagcaacgc tttgctgtac cggttttatt taattaaact
     2401 ataaaaattg tcttccgtct tctgacatct gtactctatt ttctgtactg acacattcaa
     2461 aatcggacta ttcttctcaa aatcccatcc cgtgatgctc cgcaatccgt aatagaggct
     2521 ccagtagttt tgcagttcgc ggatatagtc gcgttgcgaa cggtcttttt cggcagtggc
     2581 ggcgttgagg tcgagaacgg tggatttgcc taataaataa agacggtagg ccacatcgtt
     2641 ccggcgagcg gcacgctccg atgttttttg ggcaatggca accttatcgg cttgcaggtt
     2701 aaactgcttc acaagtttta tcacgttgga atcaaaatcg gttcgcgctt gcgcaatgtc
     2761 tatttttact ttttcgaggt tgctttttgc cacttccacc cgtccgcgcc ccacgcccca
     2821 atccaaaatc gggatgcgaa tgcccaaact aaccaattgc tggttaagcg gattgtgata
     2881 gacgttggca aaactgcggt cggtttgcga caagccgaac tgcatataca aatcagcctt
     2941 aaatccgcgc gatgccttgg cataagccac cgcactttcg ctttgaagtt tttgcaaggc
     3001 aagcagttcc atatcgggac tgttttggcg ggcaagttca agcgcttcgt tcacaggcac
     3061 cataaattcg ggtaccgctt cgcttacctg caccacaatt tccacattat ccgttattcc
     3121 cagaaaactg cgcaaatcct gaatgcggtc gtccatttca atgcgggcat tcaggcggtt
     3181 ggtttgttcg gaaaggtaat tgatttccag ttgcagcatc tcgttttcgg ttatggtgcc
     3241 tatttcgtaa cgtcctttgg cgtaagtata aagggtatcg gcattggcgt aattgtagtt
     3301 tgccgaacgt aaattgcttt gcgccgtagc c
//
LOCUS       DAOSDR010000353         3308 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrimonas sp. isolate AMR_MDS_1826
            NODE_10194_length_3308_cov_3.624962, whole genome shotgun sequence.
ACCESSION   DAOSDR010000353 DAOSDR010000000
VERSION     DAOSDR010000353.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863355
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrimonas sp. (wastewater metagenome)
  ORGANISM  Petrimonas sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Dysgonomonadaceae; Petrimonas.
REFERENCE   1  (bases 1 to 3308)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3308)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:10:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,720
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 35
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3308
                     /organism="Petrimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10194_length_3308_cov_3.624962"
                     /isolate="AMR_MDS_1826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2023866"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(465..1427)
                     /locus_tag="PLI30_11735"
     CDS             complement(465..1427)
                     /locus_tag="PLI30_11735"
                     /inference="COORDINATES: protein motif:HMM:NF012428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HOI80259.1"
                     /translation="MKNPIIPLYKQVNRLIVRSEGCCVFDSDNKQYLDFESGDWAANL
                     GHSNVKINERIKQQIDTLIHDGLRFRNNESEQLALKLLEKLGLIGGQCAFLNSGSEAV
                     NLGITIAKKLTGRKKVLKMDCSYLSAFGHGQVSDANADLINIQIDNIDAVSDLNFSEI
                     AVFVFEPGNSSSLIKYPDNDFIKAIVSEIKRHNGLLMANEVTTGFGRTGKWFGFQHYD
                     YHPNIVSVGKALGNGYPISGVAISSKMAELFQKSPFRYAQSHQNDPLGCAVGLEVINI
                     IEKDNLINQSFEKGEYFKQRLVQIQRKHDNEIIDVRARGLMIAI"
     gene            complement(1587..2138)
                     /locus_tag="PLI30_11740"
     CDS             complement(1587..2138)
                     /locus_tag="PLI30_11740"
                     /inference="COORDINATES: protein motif:HMM:NF013921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HOI80260.1"
                     /translation="MSSDNYLIANPNAVYFLTFTVTDWVDVFTRQAYKYEIVHSLNFC
                     VGNKGLTIFAWCLMSNHIHLVCRAKEGFRISEIIRDFKKFTSKSIVKLIGEIPESSRE
                     WLLYRFEYAGKFDNRITKYKFWQETNHAILLDSNSMIDQRINYVHENPVRALVVAAPH
                     EYLFSSAIDYAGGKGYVEVQVEV"
     gene            complement(2474..>3308)
                     /locus_tag="PLI30_11745"
     CDS             complement(2474..>3308)
                     /locus_tag="PLI30_11745"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HOI80261.1"
                     /translation="TAPHTPWMPTPEFRGKSQVDWYGDFVAQVDDAIGRIVRTIDDAG
                     LGNDTLVIFTSDNGGVSAGDAFSTSNLPLRGGKGYQFEGGIREPYFIKVPWLAAGGQK
                     CDTPVTGTDFYPTLLELAGIPLKPYEHADGMSLVPLLKGKTIPGRQLVWHYPHYGNQG
                     GEPSSVIRLGDWKLIHYYEDGRQELYNLRIDGQESTDVARSHPAVVGRLSKRLFAYLK
                     EVGARFPEKDPEYREELEKKHLEEVLKNRLPQLEKQRLLFLSKDFDPGNNWWGSKVTR
                     D"
BASE COUNT          842 a          815 c          667 g          984 t
ORIGIN      
        1 attatcctca acagatccgg atgcgccggc ttgccgtgcc cgtatcgggg tttaaactca
       61 actttatact ccagaaacag gttttgaaat tttggattca aaaacctgta aacctgcttc
      121 atcctgtctt gaaacttatt cattgttacc gtatcttttt tcccatttct cctgcaaaat
      181 agttcacctg cctgttgcag agatgttcag ttttttactt cagtgccctc gttttcacct
      241 caattccgcc tattgaactt aagctgcaaa caaggggtag taaagaagct taattgttag
      301 gtgccggtta ttatttcatc tattgtttct gtcaaattat caatgtgtgc tttttcaatt
      361 atcaaaggcg gcataaacct aatcgttttt tcttttaatc ctgtcagaaa tccgctgtca
      421 atcaactgac tgtaaacacc atctgcaatt tccggggttt taagctaaat ggcaatcatt
      481 aatcctcgtg ctctgacgtc aattatttca ttgtcgtgtt tcctttgtat ttgtaccagt
      541 cgttgtttga aatattctcc tttctcaaaa ctctgattta ttaaattatc tttctcgatt
      601 atatttatca cttctaatcc gaccgcacaa cccaatgggt cattctggtg agattgggca
      661 tatcgaaagg gtgatttctg aaacaattct gccatctttg aagatatggc cactccgctt
      721 atggggtagc catttcccaa tgctttccct acggacacta tattgggatg atagtcataa
      781 tgctgaaaac cgaaccattt tccggtccga ccaaacccgg tcgttacttc gtttgccatt
      841 agtaaaccgt tatgccgttt aatttcagat actatcgcct ttataaagtc gttatccgga
      901 tatttaatca ggcttgacga atttccaggt tcaaagacaa ataccgcaat ctcactaaag
      961 tttaaatctg aaacagcatc aatgttatct atctgaatat taattaaatc ggcattggcg
     1021 tcagaaacct gtccgtgtcc aaaagccgat aaataggaac agtccatttt taaaaccttt
     1081 ttcctgcccg tcagtttttt agcaatggtt attccaagat taactgcttc agaacctgaa
     1141 ttgagaaaag cacattgccc gccgattagc cctaattttt caagtaattt taaagctaat
     1201 tgctccgatt cattatttct aaaccttagt ccgtcatgta tgagcgtgtc aatttgttgt
     1261 tttattcgtt cgttaatctt tacattggag tgcccaagat ttgcagccca gtcgcctgat
     1321 tcaaaatcaa gatactgttt attatctgaa tcaaacacac aacacccctc cgacctaaca
     1381 atcaacctat taacttgctt atacaatggt attattggat ttttcattct ttattttttc
     1441 ttgtttacag aacgttcttt aaactgacat ataacagttt ggtgtaaatt tttgtagcgg
     1501 attctccatc tgcccccatc taccccatct acccctcgtt tgtaacgagg gggaagttgt
     1561 ggatcgtttt aaacgataca attctttcat acttctacct gcacttccac gtatcctttc
     1621 cctcctgcat aatcaattgc cgaactaaac aaatattcgt gtggcgcagc aacaaccagc
     1681 gcccttaccg gattttcatg aacatagttg atccgctggt caatcatact gttgctatcc
     1741 agtaaaatgg catggttggt ttcctgccag aacttatatt ttgttatccg gttgtcaaat
     1801 ttcccggcat attcaaaccg gtacaataac cactcgcgac tactttccgg aatttcacca
     1861 atgagtttta cgattgactt ggaggtaaat ttcttaaaat cccggatgat ttcacttatt
     1921 ctaaatcctt cttttgccct gcaaaccaaa tgtatatgat tcgacattaa gcaccaggca
     1981 aaaatggtca accccttatt accgacacaa aaatttaacg aatgaacaat ttcgtattta
     2041 tacgcttgcc gtgtaaaaac atctacccag tcggttacgg tgaaggttaa aaaatatacc
     2101 gcattgggat tggcaatcaa ataattatca cttgacattt tcagatataa actctcaatt
     2161 ttcaactatt cccttgcggg cataaattcc tgcattacaa atgccagaca atgcacccct
     2221 cgttacaaac gaggggtagt gaagggtggg cgatcggagc agttggagtg acagcatgtt
     2281 agtccggttt agatttgact tccaaattac aaaaccggac tggctaaaac aatagttttc
     2341 agggaaaaat gaagctaaat tctaaggagt gtactgaggg aaaaaccgct cagctggctt
     2401 cgacggcaag cagccgctgc agccgccggc agaacatagc aaccctggcg cacgggagtg
     2461 tcaaaaccgg gagttagtcg cgggtaacct tactccccca ccagttgttt ccggggtcaa
     2521 agtctttcga tagaaacagc agccgctgtt tttccagctg cggcaaccgg tttttcaaga
     2581 cctcttccag gtgctttttc tcaagttctt cgcggtattc cgggtctttt tcggggaaac
     2641 gggcgcccac ctccttcagg taagcaaaca gccgcttgct gagccggcca accacggcgg
     2701 ggtgactgcg ggccacatcg gtgctctcct gcccgtcgat gcggaggttg taaagctcct
     2761 gccgtccgtc ttcgtaatag tggatcagct tccagtcgcc cagccggatc accgacgaag
     2821 gttcgccgcc ctggtttccg taatgcggat agtgccacac caactgcctt ccggggatgg
     2881 ttttaccttt cagcagcggc accaggctca tgccgtcggc atgttcgtac ggtttcaacg
     2941 ggattcccgc caattcgaga agggtaggat aaaaatcggt gccggtaacc ggcgtgtcgc
     3001 atttctgtcc gccggcagcc agccacggca ctttgataaa atagggttcg cggatcccgc
     3061 cttcaaattg gtaccctttt ccgccccgta gcggaaggtt tgaagttgaa aaggcgtcgc
     3121 cggccgaaac gcctccgttg tcggaggtga agatgaccag cgtgtcgttg cccaagccgg
     3181 cgtcgtcaat cgtccgcacg atccgaccga tcgcgtcgtc cacctgcgca acgaaatcgc
     3241 cataccagtc gacctggctc ttgcctcgaa actccggcgt cggcatccag ggcgtgtgcg
     3301 gcgcggtc
//
LOCUS       DAOSDR010000354         3302 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrimonas sp. isolate AMR_MDS_1826
            NODE_10212_length_3302_cov_3.848476, whole genome shotgun sequence.
ACCESSION   DAOSDR010000354 DAOSDR010000000
VERSION     DAOSDR010000354.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863355
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrimonas sp. (wastewater metagenome)
  ORGANISM  Petrimonas sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Dysgonomonadaceae; Petrimonas.
REFERENCE   1  (bases 1 to 3302)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3302)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:10:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,720
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 35
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3302
                     /organism="Petrimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10212_length_3302_cov_3.848476"
                     /isolate="AMR_MDS_1826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2023866"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            141..908
                     /locus_tag="PLI30_11750"
     CDS             141..908
                     /locus_tag="PLI30_11750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019539991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03915 family putative DNA repair protein"
                     /protein_id="HOI80262.1"
                     /translation="MIIFTYDKTFDGLLSCVFFAYEQKKFPDFILSESDQKPLFVDEQ
                     YRIITEKEKSLRVWKALEKKLSKIAQNMMLSVWLSELPETEMLLFRYIRKNIDHPEGV
                     EMNFGDDDVLRIKEIAQKVAKEAEQLRQFVRFQETADGIYFAPVSPRYDVLSLIVSHF
                     QSRYAGQPWIIYDTNRNTGLYYDTRSVVEVSFSQKDLSNLRLGVLDEEKLSSDETFFQ
                     QMWKEYFKSTTIKERINLKLQRQHMPRRYWRYLTEMQ"
     gene            complement(966..1382)
                     /gene="mscL"
                     /locus_tag="PLI30_11755"
     CDS             complement(966..1382)
                     /gene="mscL"
                     /locus_tag="PLI30_11755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019539990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="large-conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HOI80263.1"
                     /translation="MSFRTELKEFLMRGNVVDMAVGIVIGAAFGKIVTSFVNDILMPP
                     IAMLVGDSKFAELKVVLKEAVMSGADVVAPAITWNYGAFIQAVVDFVIVGTAIFLVIK
                     AMNSMKRKKEEAPAAAPEPTKEETLLTEIRDLLKNK"
     gene            complement(1478..2380)
                     /locus_tag="PLI30_11760"
     CDS             complement(1478..2380)
                     /locus_tag="PLI30_11760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019539989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase-related protein"
                     /protein_id="HOI80264.1"
                     /translation="MQTTSRILMVRPARFDFNEETAKNNYFQQKTGQEGVSEKALEEF
                     DAFVNLLRRNDVEVFVMQDTEEPWTPDSLFPNNWFSSHLSGELVLYPLFAENRRRERK
                     PHVLDFLRRKMNHTKLIDLTPWEEKGEFLEGTGSMVLDRDKRIAYCCRSPRSSEKVLA
                     DFCSRMNFDAVVFDAVDKNGNLIYHTNVMMEVGTQVAVVCLESIRNGEERQKVESRLS
                     ATGKVIVEISPNQVEHFAGNMLELKSRNGAPLMIMSTTARKSLTMQQEKTISTYNKIL
                     SPELTTIETNGGGSARCMIAELFH"
     gene            complement(2456..>3302)
                     /gene="rocD"
                     /locus_tag="PLI30_11765"
     CDS             complement(2456..>3302)
                     /gene="rocD"
                     /locus_tag="PLI30_11765"
                     /EC_number="2.6.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004587 - ornithine-oxo-acid transaminase
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ornithine--oxo-acid transaminase"
                     /protein_id="HOI80265.1"
                     /translation="GIPENEAIIVACEGNFHGRTITIVSLSTDGEARAEYGPYTPGIE
                     VIPYNDVDALQQIFEVKGDKIAGLLVEPIQGEAGVFVPDDGYLKACKHLCEQHDILFI
                     ADEVQTGIARTGKLLCCDHEGIRPDIVILGKAISGGVMPVSAVLADDEIMMTIKPGQH
                     GSTFGGFPIACKVATAALEVVKEERLAEKAGYLGEIFRNEMRKIDSPFVHQVRGKGLL
                     NAIVIEPHNGKEAWDVCLKMAENGLLAKPTHQHIIRFAPPLVITEEEIREAIEIIKES
                     IKMLE"
BASE COUNT          838 a          822 c          747 g          895 t
ORIGIN      
        1 catcgtgtgc cgcgaactgc caatgcgcac cgtgaacgaa ctcacacctc agtatgtccg
       61 tcgacaggtt tcccaaaaac agaaaaaaca ggctgcagat ttgttgcagt attcgattaa
      121 ctggggagag tgatagcaca atgataattt ttacctacga taaaactttc gacggccttc
      181 tctcctgcgt gtttttcgca tacgagcaga agaaatttcc cgattttatc ctttcggaat
      241 ccgatcaaaa gccgttgttc gttgacgagc aatatcgtat aattacagaa aaggaaaaat
      301 cgctacgtgt atggaaagca ctggaaaaaa aactttcgaa aattgcgcag aacatgatgt
      361 taagcgtttg gctgtcggaa ttacctgaaa ccgaaatgtt gcttttccgc tacattcgta
      421 agaatatcga ccatccggag ggtgtcgaaa tgaattttgg cgacgacgat gtgcttcgta
      481 ttaaagaaat tgcgcaaaaa gtagctaaag aagccgaaca gcttcggcaa ttcgttcggt
      541 ttcaggaaac ggccgacggg atttatttcg ctcctgtatc accgcgttac gatgtgctct
      601 cgttgatcgt ttcgcatttt caatcccggt atgccggcca gccttggatt atctacgaca
      661 caaaccgcaa cacaggcttg tattacgata cacggtccgt tgttgaggtt tccttttcgc
      721 agaaggacct ctcgaattta agactcgggg ttctcgacga agaaaaactt tctagcgatg
      781 aaacattttt tcagcaaatg tggaaagaat attttaaaag cacgactatt aaggagcgca
      841 tcaatctgaa actgcaacgg cagcacatgc cccgtcgtta ctggaggtac ctgacggaga
      901 tgcagtgaaa aaccacccgg ccaataggcg gcccggcggt tcaaaaaagt tacccggatc
      961 ttttcttact tgtttttcaa tagatcgcgg atttcggtca gcaacgtttc ttccttggtg
     1021 ggttccggag ctgcagccgg agcttcttct tttttacgct tcattgagtt catggctttg
     1081 atcaccagga agatagctgt gcccacaatc acaaagtcaa caaccgcctg gatgaacgcg
     1141 ccgtaattcc acgttatggc cggagcaaca acgtctgctc cgctcatgac cgcttcttta
     1201 agaaccactt tcagctcggc aaacttgctg tcaccgacca gcatagcgat gggcggcatc
     1261 aggatgtcgt taacaaaaga ggtgacaatc ttgccgaaag cagctccgat aacaataccc
     1321 acggccatat ccactacgtt gccgcgcatt aaaaattcct taagctctgt tctgaatgac
     1381 ataatactcg tttattaaaa ggttaatttt ccatttcatc acaaagaaaa caaaaaaatg
     1441 tgaatatatg gtttttatcg ataaatttca ctccttatca gtggaatagt tccgcaatca
     1501 tacaccgggc agagccaccg ccgttggttt cgatagtggt aagttcaggc gaaagaattt
     1561 tattataggt cgaaatagtc ttttcctgct gcatcgtcag cgacttccgg gcagtagtcg
     1621 acatgatcat caggggagct ccgttcctgc tctttaactc cagcatgttc cctgcaaaat
     1681 gttccacttg gttcggggag atctccacga taacttttcc cgttgcactc agtctcgatt
     1741 ccactttctg gcgttcctcc ccgttcctta ttgattcaag gcatacaacg gcaacctgag
     1801 tacctacctc catcattacg ttcgtatggt agataaggtt tccgtttttg tcgacggcat
     1861 caaaaaccac cgcatcgaaa ttcattcgtg aacaaaaatc agccagcact ttttccgacg
     1921 accgcgggga gcggcagcag taggcaatcc gtttgtcgcg gtcaaggacc atgctccccg
     1981 taccttccag aaactcgcct ttctcctccc acggtgtgag atcgataagt ttagtgtggt
     2041 tcatcttccg gcgcaggaaa tccagcacgt gtggcttcct ctcccggcgg cggttttcgg
     2101 caaacagggg gtaaagaacc agctcccccg aaaggtgaga ggagaaccag ttgttgggaa
     2161 acagggagtc gggcgtccag ggttcttccg tatcctgcat gacaaacacc tccacgtcgt
     2221 ttctccggag tagattcacg aaggcatcaa actcctccag cgctttttcc gacactcctt
     2281 cctggcctgt cttttgctgg aaatagttgt tttttgccgt ttcttcgtta aaatcgaacc
     2341 gggccggacg gaccatcaat atccttgacg ttgtttgcat aataataaga ttgaattagg
     2401 ttgaagcaga ttgactgaga ttgattaagg acggtttcaa tctttctcaa tctatttact
     2461 ccaacatttt aattgattcc ttaataattt caattgcttc cctgatttct tcttccgtga
     2521 ttaccaacgg cggagcaaag cggatgatgt gttggtgtgt aggttttgcc aacaagccgt
     2581 tttcggccat tttcagacag acatcccagg cttctttacc gttgtgaggt tctataacaa
     2641 tggcattgag caatcccttt ccgcggacct ggtgtacaaa cggactgtca atcttcctca
     2701 tttcgttgcg gaagatctct cccaggtacc cggctttttc cgccagcctt tcctctttta
     2761 ccacttccag cgcagcagta gctactttac aggcaatcgg gaatcctcca aaagtagatc
     2821 cgtgctggcc gggtttaatg gtcatcatga tctcgtcgtc ggcaagcacg gcagaaaccg
     2881 gcataacgcc accggagatg gctttaccca gaataacgat gtcgggacga attccctcgt
     2941 ggtcacaaca caaaagcttg cctgtgcgtg cgattccggt ttgcacttca tcagcgatga
     3001 acagtatgtc gtgctgttca cagaggtgtt tacaagcttt caggtatccg tcatcgggaa
     3061 cgaaaacacc ggcttcaccc tgaatgggtt caacgaggag tccggctatc ttatctcctt
     3121 tcacttcaaa aatctgctga agagcatcca catcgttgta ggggatgact tcaatccccg
     3181 gcgtgtacgg gccgtattcg gcacgggctt ccccatcggt ggaaagggag acgattgtaa
     3241 tggtacgccc gtgaaaattt ccttcgcaag cgacaataat ggcctcattt tcggggattc
     3301 ct
//
LOCUS       DAOSDR010000355         3279 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrimonas sp. isolate AMR_MDS_1826
            NODE_10317_length_3279_cov_4.077854, whole genome shotgun sequence.
ACCESSION   DAOSDR010000355 DAOSDR010000000
VERSION     DAOSDR010000355.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863355
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrimonas sp. (wastewater metagenome)
  ORGANISM  Petrimonas sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Dysgonomonadaceae; Petrimonas.
REFERENCE   1  (bases 1 to 3279)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3279)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:10:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,720
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 35
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3279
                     /organism="Petrimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10317_length_3279_cov_4.077854"
                     /isolate="AMR_MDS_1826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2023866"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..765)
                     /locus_tag="PLI30_11770"
     CDS             complement(<1..765)
                     /locus_tag="PLI30_11770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009133036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HOI80266.1"
                     /translation="MDRRSFIIRSAALSAGLVATGGVPLLGSDTLVDTNGKRRANIYT
                     TFKNPDAKYHPFVRWWWNGNKVEKNEIIRELRLLKEAGVGGVEINPIDFPSTGDDLGI
                     KSIRWLSDEWIDLLQVTFNEAKKLDMTCDLIVGSGWPFGAETLPHDERAQVALILAEE
                     LEGPMDYEVSRYHIFNETDPGVTDTFPGRTFEILSLKMVPEPMESLDQVIELSDQRHE
                     ELIQVKVPEGKHVFYALVKVNSFASVINGAPGAAGPI"
     gene            complement(829..2445)
                     /locus_tag="PLI30_11775"
     CDS             complement(829..2445)
                     /locus_tag="PLI30_11775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007658333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI80267.1"
                     /translation="MCFLVSWGILLSLQVNSAEPERRYRIIHNNDGSDALGNYWFHKR
                     PLTVHDVNAYVDMVSHSQVTTYMMCTGSDFFYYRSKHGRILGDDLDGKLDCGNDTAAT
                     HHYKSFYLNHLNLEKDGTDLIEASLKRAKMHGMEAFITYRMNDLHFNDTTSNCPITYP
                     EFWYTHPEFWTNDDTPGLNGSRALDFAHPEVRAHKLAIITEQLERYEMIDGLDLDFMR
                     FIVYFKQGEGKKNAFLMTELMKEVKAKVDELSEKRGKKILLSVRVPLTVRDCLDKGLD
                     IQEWIRLGLVDFITIGVHWRGNPSLPVAQFIKELGSTTVPVYATIDDGGYKPRETFSH
                     GMFRGMASHILSQGADGIYLFNHYYGAFNSTYQGLLHLEEGGQVCRVISPHLIQELGS
                     PETLAYRNKIYCLSDGVTEYGIKHISSLPLEVRSDDFSKAEIFIGDDPGKTVPEEVIL
                     FVRSSKQASFTLKVNGRDITHSEPDYVSLYDKGRGLMEGECQYAFVLPVESIQQGYNT
                     VAFHAADSSLLVKRVEIAIKYGDVKTHGYF"
     gene            complement(2623..>3279)
                     /locus_tag="PLI30_11780"
     CDS             complement(2623..>3279)
                     /locus_tag="PLI30_11780"
                     /inference="COORDINATES: protein motif:HMM:NF019592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HOI80268.1"
                     /translation="WVDGNGAQTYQFSNFNCFLEGNGGLSAYHMRIDNRLYEKMDDND
                     YRKSRFMTTPVAYTYPTDNRERVLPKYTNLKWGATIAKQHNVRNNQLNCDYTYIRSSE
                     VLLMLAEAQALAGQEGAAKATLNKLLAARTKEGSPTLTCDNYSSMAGLSVLDMVKLQW
                     RIEMWGENGLEYSNSKRWNVDIDRNGSTVHWSSGKIYKVEHMTYEIPIQETSTNPNWF
                     "
BASE COUNT          840 a          759 c          723 g          957 t
ORIGIN      
        1 gataggccct gcagcaccgg gtgcaccgtt gatcacagag gcaaaggagt tgactttcac
       61 caaggcataa aagacatgct tcccttccgg caccttaacc tggataagtt cttcatgccg
      121 ctggtccgat aattcaatca cctggtcgag actctccatg ggttcaggaa ccattttcag
      181 ggaaagaatt tcaaaagtcc tgcccggaaa ggtgtcagta actccgggat ccgtctcgtt
      241 gaaaatatgg taacgcgaga cttcatagtc cattggccct tccaattcct cagcgagtat
      301 cagtgccacc tgggcacgct cgtcatgggg taacgtttct gcaccgaaag gccaacctga
      361 acctacgatc agatcacagg tcatatccag ttttttagct tcattgaagg taacctgcaa
      421 caggtcgatc cattcgtcac tgagccaccg tatcgatttg atgccgagat catctccggt
      481 tgaagggaag tcgatagggt tgatctcaac cccacccaca cccgcttctt tcaacaggcg
      541 taactctctg ataatctcat ttttttctac tttgtttccg ttccaccacc acctgacgaa
      601 aggatggtat ttggcatccg gattcttaaa tgtggtatag atattggctc tacgctttcc
      661 gtttgtatcc acaagtgtgt cggatcccaa caggggaaca cccccagtgg ctaccagacc
      721 ggcggaaagt gccgcactac gaatgataaa agatcgtctg tccatatttt ttttaatact
      781 attcgagttg tttttagaaa ctgtttaaat tttttggttg caggttcgtt aaaaatatcc
      841 gtgtgttttt acatctccgt atttaattgc gatttccaca cgtttcacca ggagagatga
      901 gtctgctgcg tggaaagcga cggtattgta tccttgttga atactttcta cgggtaggac
      961 aaatgcatac tggcattctc cttccatcag tcccctacct ttgtcataaa gtgagacata
     1021 atccggctcc gaatgggtta tgtccctgcc gttgactttt aatgtaaagg atgcctgctt
     1081 actgctcctg acaaagagta tcacctcttc gggaacggtc tttccgggat catccccaat
     1141 aaaaatttcg gctttggaga aatcatcgga tcttacctcc aagggtaagg aggagatgtg
     1201 tttgatgcca tattcggtta caccatcgga gaggcaatag atcttgttcc tgtaggcaag
     1261 tgtctccggg gatcccagtt cctgaataag atgaggtgag atgacccggc atacctggcc
     1321 tccctcttca aggtgcaaca atccttggta agttgagtta aaagctccat agtaatgatt
     1381 gaaaagataa ataccatcgg caccctgcga taagatatgg gaagccattc ccctgaacat
     1441 gccgtgcgaa aaggtctctc gaggtttgta ccctccgtca tcgatggttg catagaccgg
     1501 gacagttgtt gatcccagtt ctttgataaa ttgtgcaacc ggaagggaag ggttgccacg
     1561 ccagtgcact ccgatggtta tgaagtcgac caaccccaga cggatccact cctggatgtc
     1621 gagccccttg tcaaggcaat cccggaccgt gagtggtacc ctgacggaaa ggagtatttt
     1681 ttttcctctt ttttcagaga gttcatccac tttggctttt acctctttca tcagttcggt
     1741 catcaggaaa gcgtttttct ttccttcgcc ttgtttaaaa tagacgatga aacgcataaa
     1801 atccagatcc aatccgtcaa tcatctcata tctctccagt tgctcggtaa tgatcgccaa
     1861 tttatgggca cgtacttccg gatgtgcaaa atccaatgcc ctggaaccat ttaatccggg
     1921 ggtgtcatcg ttggtccaaa actcaggatg tgtataccag aattcgggat aagttatggg
     1981 gcaattggac gtagtatcat taaaatgaag atcgttcatc ctgtaggtga taaaagcttc
     2041 cataccgtgc atctttgctc gtttaagtga tgcttcaatc aggtcagtac catctttttc
     2101 gagattcagg tggtttaggt aaaaactttt gtaatgatgg gtagcagccg tgtcattacc
     2161 acaatccagc tttccgtcaa gatcatcacc caatatacgg ccatgctttg agcggtaata
     2221 aaaaaaatcg ctgcctgtgc acatcatgta ggtagtcact tgtgaatggc tgaccatgtc
     2281 gacgtaagca ttcacatcat gcacggtgag gggccgtttg tggaaccaat aattgcccag
     2341 agcatcactt ccgtcgttgt tgtgaataat ccggtatctc ctttctggtt cagctgaatt
     2401 aacttgtaag gatagaagta tgccccaaga taccaaaaag cacacgatat attttttttg
     2461 catagtactg cccggatgat aagggtgttt tgaaattgtt acaaaaaata ccggatctga
     2521 ttcgaggaat tgcacaaatc agataatttt agaatgaaga gggtagggat taagaaatct
     2581 ctaccctctt tattaattag aaaatttatg agaacataga atctagaacc aattcgggtt
     2641 tgtagaggtc tcctgaatag ggatttcata ggtcatatgt tccactttgt agatttttcc
     2701 ggatgaccag tgaaccgttg atccgttacg gtcaatatct acgttccagc gtttgctgtt
     2761 ggaatattca agcccgttct ctccccacat ttcgatcctc cactgcagtt tgaccatatc
     2821 cagcacagaa aggccagcca tagacgagta attgtcacac gtcaatgtcg gtgaaccctc
     2881 ttttgttctt gcggcaagta atttgttaag cgttgctttt gcagcaccct cctggccggc
     2941 aagagcctgt gcttcagcca gcatcaacaa tacttccgaa gaacggatat aggtatagtc
     3001 acagtttagt tgattgttcc tgacgttatg ctgtttggca atggtagcac cccatttcag
     3061 gttggtgtat ttgggaagca ccctttctct attgtccgta ggataagtat aagctaccgg
     3121 tgttgtcata aacctgcttt tacggtaatc gttgtcatcc atcttttcat acaacctgtt
     3181 gtctattcgc atatggtatg cacttaaacc tccgttgcct tccaggaagc agttgaaatt
     3241 gctaaactga taggtttgag ctccgtttcc gtcaaccca
//
LOCUS       DAOSDR010000356         3274 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrimonas sp. isolate AMR_MDS_1826
            NODE_10342_length_3274_cov_4.257223, whole genome shotgun sequence.
ACCESSION   DAOSDR010000356 DAOSDR010000000
VERSION     DAOSDR010000356.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863355
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrimonas sp. (wastewater metagenome)
  ORGANISM  Petrimonas sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Dysgonomonadaceae; Petrimonas.
REFERENCE   1  (bases 1 to 3274)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3274)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:10:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,720
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 35
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3274
                     /organism="Petrimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10342_length_3274_cov_4.257223"
                     /isolate="AMR_MDS_1826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2023866"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..196)
                     /locus_tag="PLI30_11785"
     CDS             complement(<1..196)
                     /locus_tag="PLI30_11785"
                     /inference="COORDINATES: protein motif:HMM:NF013530.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HOI80269.1"
                     /translation="MKTILITGGSGLIGRKLSRLLVEKGYKVIWLSRERYVKDDIPRY
                     RWDYRRNEIEKEAVELADIIV"
     gene            complement(264..980)
                     /locus_tag="PLI30_11790"
     CDS             complement(264..980)
                     /locus_tag="PLI30_11790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019540384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HOI80270.1"
                     /translation="MKTIYHAAGSRGFADHGWLKSNHTFSFANYYNRDRMHFGVLRVI
                     NDDFVDKGKGFGTHPHNDMEIISLPLEGELEHRDSMGNGSIIRQGDVQVMSAGTGITH
                     SEFNARQDQPVEFLQIWVFPREKGVKPRYDQVRIADLAKPNDFQQIVSPNPDGDGVWI
                     HQDAWFHLANFDKGVTKEYDVKKKGNGVYLFVIRGKAKVSTQTLNERDGYGIWDIGSF
                     TLEALEDSEILLMEVPMELP"
     gene            complement(1373..2056)
                     /locus_tag="PLI30_11795"
     CDS             complement(1373..2056)
                     /locus_tag="PLI30_11795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019540383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HOI80271.1"
                     /translation="MSAVWFLLILIAVAGFGVQAMLQSRFKKYSRIPLRNGMTGRDVA
                     EKMLHDNGIYDVQVISVRGQLTDHYNPTNKTINLSEPVYGSNSIAAAAVAAHETGHAL
                     QHAKGYAPLKMRSALVPVVSASSRWVTWILLLGIVTVSTFPALLWMGIGLFALTTLFS
                     FVTLPVEKNATNRALRWLSSAGVTDVSNHSQATDALRWAGYTYVVAALSSLATLLYYI
                     MIAMSGSRR"
     gene            complement(2138..2434)
                     /locus_tag="PLI30_11800"
     CDS             complement(2138..2434)
                     /locus_tag="PLI30_11800"
                     /inference="COORDINATES: protein motif:HMM:NF013315.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI80272.1"
                     /translation="MDEPLPIASGQTISQVTTVALQTGLPRWAPFNGILITCGAPKIP
                     EELLGQLVTGGRMVVPIGEKLREMVVVEKTGETAYTTTGEGFFRFVPMLKGTVE"
     gene            complement(2455..>3274)
                     /locus_tag="PLI30_11805"
     CDS             complement(2455..>3274)
                     /locus_tag="PLI30_11805"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005858796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="adenylosuccinate synthetase"
                     /protein_id="HOI80273.1"
                     /translation="GDLFNNFEEKYKKATARHIEMLAQYDFEYDLEKIETEWFAGVEK
                     LKSFQVIDSEHFINKSLTSGKGVLAEGAQGSMLDIDFGSYPFVTSSNTICAGACTGLG
                     VAPRKIGEVFGIFKAYCTRVGSGPFPTELFDKDGQQMRDLGREYGSVTGRPRRCGWID
                     LVALRYAIMLNGVTKLVMMKSDVLDTFETIKVCVAYNINGQETEDLPFDITGNIEPVF
                     VELAGWKTDMTNMQSEDEFPEEFNAYLSFLEEELGVPVAIVSVGPNRAQTIIRG"
BASE COUNT          842 a          850 c          651 g          931 t
ORIGIN      
        1 ggacaataat atcggccagt tccacggcct ctttctctat ttcgtttcga cgatagtccc
       61 acctgtaccg gggtatatca tcttttacat atcgctcacg gctaagccaa atcactttgt
      121 atcctttttc aacaagcaac cgtgaaagtt ttcttcctat taaacctgat ccacctgtta
      181 ttaaaattgt tttcatcgca gtgtagttaa tttcatggta aacaaataaa gacagcaatt
      241 ggttgccgtc tttatccata atttcaaggt aattccatgg ggacttccat caaaagaatc
      301 tcggaatcct caagagcttc aagcgtaaaa ctaccgatat cccaaattcc ataaccgtca
      361 cgctcgttca acgtttgggt cgaaactttg gctttgcctc taatcacaaa tagatatact
      421 ccgttacctt tcttctttac gtcatattct ttcgtaaccc ccttgtcaaa attggcgagg
      481 tgaaaccatg catcctgatg tatccataca ccgtcgccgt ccggattcgg agaaacgatt
      541 tgctgaaaat cgttgggttt ggctaaatcg gcaatacgga cctgatcgta acgcggcttg
      601 acgcctttct ctctcgggaa cacccagatt tgcaggaatt ctaccggctg atcctgacgg
      661 gcattaaatt cactgtgcgt aatccctgtt ccagccgaca taacctgcac gtctccctga
      721 cggataatgc ttccgttgcc catactgtcg cgatgttcga gctcaccctc gagcggcaag
      781 gatattattt ccatatcgtt gtgcgggtgc gtaccgaaac cttttccctt atccacgaaa
      841 tcatcattga ttacccgcaa aacaccaaaa tgcattctgt cgcgattgta ataattggca
      901 aaactgaatg tatgattact tttgagccat ccgtgatcgg caaagccgcg gctacctgca
      961 gcatgataaa ttgttttcat tttttctttt ttgtgatttt taaaatattc tccatgcaaa
     1021 gataaccgct ctttttatag tgtgcattga tgtatgttaa gaaaagactt caggcttagc
     1081 gtgggcgata gtttcgctct gccagctctc ccctcacccg gctcaaactt tcaggagtaa
     1141 tgcccagata ggcagcaatc atccttgctc ttcagccgaa gcacctagca gttgggttac
     1201 ccgcgtttgc aggtttaaaa tatgactgtg aagcaaactc gcctttccta agtgtgagcg
     1261 ttttacaact ttccggcaac aaactgatag cctgctcgtc aatatctata ttcgattcga
     1321 gaatgttctc cgcaccgcgt ccaatattat tttgcccccg tcaattaaaa atttatctcc
     1381 tgctcccgct catggcaatc atgatgtaat acagaagagt tgccagcgaa cttaatgcag
     1441 ccacaacata agtatacccg gcccaccgta aagcatcggt ggcctgcgag tggttgctta
     1501 catcggtaac accggcgctg cttagccacc ttaacgccct gttggtcgca tttttttcta
     1561 cgggaagcgt cacaaaacta aacagggttg tcaaggcaaa caagccgatc cccatccaca
     1621 acaatgccgg gaaggtagat actgttacaa ttccaagcaa caatatccac gtaacccacc
     1681 ttgaagaagc gctgacaacg ggtaccaaag ccgaacgcat tttcagaggg gcataccctt
     1741 tggcgtgctg caaggcgtga cctgtttcat gagcagccac tgcagctgca gcgatactgt
     1801 tgctcccgta aaccggttcg ctcaggttga tggttttatt ggtggggtta tagtgatcgg
     1861 taagctgacc gcgtaccgaa atgacctgga catcgtatat gccgttgtcg tgaagcattt
     1921 tttctgccac gtcacgaccc gtcattccgt ttctcagggg aattcttgaa tattttttaa
     1981 accggctctg gagcatagct tgtacaccaa agccagctac agcaattaaa attaatagaa
     2041 accaaactgc actcatgcga ttcttttttt atttagtttt tttgttaaac gtaaaacagc
     2101 tataaaatgt tttctacgaa ttagacctgt ttgtttttca ctccaccgtt cctttcagca
     2161 tgggtacaaa ccgaaagaag ccttcaccgg tagtcgtgta ggccgtttca ccggtttttt
     2221 caaccaccac catctcccgc aatttttctc ctatcggcac taccatcctt cctcctgtca
     2281 caagttgtcc cagcagttct tccggaatct tgggtgcccc acaggtaatt aatatcccgt
     2341 tgaaaggagc ccatcgaggc aacccggtct gcagggctac ggtagtaacc tgagaaatag
     2401 tttgtcccga cgcaataggt aacggctcat ccaagtaagc cagatcggat gtttttatcc
     2461 cctgatgatt gtctgagctc tgttcggccc tacagaaaca atagcaacag gcacacccag
     2521 ctcttcttcc aaaaacgaaa gataagcgtt aaattcttcg ggaaactcgt cttccgactg
     2581 catgttcgtc atatcggtct tccaacccgc cagttctacg aaaacaggtt caatgtttcc
     2641 tgtaatgtcg aaaggcaagt cttcggtttc ttgtccgttg atgttgtatg ccacgcacac
     2701 cttgatggtt tcgaaagtat ccagcacatc acttttcatc atcaccagct tggtcacccc
     2761 attgagcatt atcgcatagc ggagtgctac caggtcaatc catccgcaac gacgtggacg
     2821 tccggtaacg gaaccgtatt cacgacctag atcacgcatc tgctgtccgt ctttatcgaa
     2881 cagttctgta gggaaagggc cacttcccac ccgggtgcag tatgccttaa aaatcccgaa
     2941 cacttcgcca atttttcgcg gagccacacc caatccggta caggcgccgg cgcaaatagt
     3001 gttggatgaa gtaacaaacg gataggaacc aaaatcaata tcaagcatag atccttgcgc
     3061 accttcagca agcactccct ttcccgatgt gagtgacttg ttgataaaat gttccgaatc
     3121 gataacctgg aatgatttca atttctcaac tccggcaaac cattcggttt caatcttttc
     3181 caagtcgtac tcaaaatcgt attgcgccag catctctatg tgtcttgccg tagctttttt
     3241 gtatttctct tcgaaattat tgaataaatc tcct
//
LOCUS       DAOSDR010000357         3273 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrimonas sp. isolate AMR_MDS_1826
            NODE_10348_length_3273_cov_4.502797, whole genome shotgun sequence.
ACCESSION   DAOSDR010000357 DAOSDR010000000
VERSION     DAOSDR010000357.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863355
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrimonas sp. (wastewater metagenome)
  ORGANISM  Petrimonas sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Dysgonomonadaceae; Petrimonas.
REFERENCE   1  (bases 1 to 3273)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3273)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:10:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,720
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 35
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3273
                     /organism="Petrimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10348_length_3273_cov_4.502797"
                     /isolate="AMR_MDS_1826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2023866"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..368
                     /locus_tag="PLI30_11810"
     CDS             <1..368
                     /locus_tag="PLI30_11810"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016268365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOI80274.1"
                     /translation="GIADRFHFTGFLRGEQVYEMLKSSDVYVMPSVSEPFGISPLEAM
                     QCGVPSIISYQSGCAEILNNVIKTDYWDVDAMADAMYSICTYPGMAEFLKEEGKREVD
                     SIVWEDAGKKIRAIYDSLV"
     gene            408..1736
                     /locus_tag="PLI30_11815"
     CDS             408..1736
                     /locus_tag="PLI30_11815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009318561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 57 protein"
                     /protein_id="HOI80275.1"
                     /translation="MKTICFYFQIHQPYRLKRYRFFNIGRDHYYYDDYSNEDIMQQIA
                     ARSFIPANRMLLDLINTYKGKFKFAISVSGVALDQMEVYAPEVIDGLKELSRTGAVEF
                     LAETYSHSLSSLIDPVEFQSQVQQQTQKIKLLFDQKPKVLRNTEMIFSDDIAEMAYNM
                     GYTKMVSEGAKLILSWRSPNYVYKSENQPNMKLLLRNSQFSDDISLRFSDYTWKEYPL
                     TAEKYISWIAATSPQEEVFNIFMNYETFGNLQPAHSGIFEFMKALPKFAFDKGIEFST
                     PSQIMDTHKPVGTVSVPLAISWSDEERDLSRWLGNKLQKSAFNTLYEISERIRLANDR
                     RLKKDWQYLQSSDHFYYMSTKHFYDGSMHSKFSPYQSPYDAFNNYMNVLSDFIDRVKA
                     QFPDSVDNEELNSLLTTIHNQEREIKKLQSELKKVLTENEEKLVEKTPVK"
     gene            1871..>3273
                     /gene="nhaD"
                     /locus_tag="PLI30_11820"
     CDS             1871..>3273
                     /gene="nhaD"
                     /locus_tag="PLI30_11820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010800010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter NhaD"
                     /protein_id="HOI80276.1"
                     /translation="MIYAMILLFIAGYFFIAMEHTIKVNKASVALLIGTLLWVIYVYL
                     APSTVLSVSPDAFNHYIHSHPELQHHAFGIQVRHFVIDHQILESIGDISEMLFFLLAA
                     MTIVELIDVHGGFTYITEKITTRKKRRLLWLISFITFFMSAVLDNLTTTIVMIMLTRR
                     IIREPRERWLFGSIIVIAANSGGAWSPIGDVTTIMLWVKGNITASIIPQLFFPSLVSM
                     IAPVFVAQFLLKGNIQEEEISQTEEKENSELLLHMQKKDRLGILIFGVSILVLSPVFK
                     GVTHLPPFIGLLLGVSLLWIYTEILYNQKNQLNENLKLRVSKVLGRIDFSTLMFFLGI
                     LLAVDALQSAGILQQSSAFMAQHLPNVYAQAYAIGLLSAIVDNVPLVAAAIGMYPVLD
                     PAALSTMADPVFMQNFVEDGVFWHFLAYCAGVGGSILIIGSAAGVVFMGLEKVPFGWY
                     LKRISLIALIGYTFGAGA"
BASE COUNT          938 a          710 c          697 g          928 t
ORIGIN      
        1 ggggaattgc tgacagattc cactttaccg ggttccttcg aggcgaacag gtctatgaaa
       61 tgctgaaatc gagcgatgta tatgttatgc cttcggtatc agaacctttt ggtatttcac
      121 cgcttgaagc tatgcagtgc ggtgtgccaa gtataatctc ctaccagtcg ggttgtgcag
      181 agatactgaa taacgtcatt aaaaccgact actgggatgt tgacgccatg gcggacgcca
      241 tgtattcgat ttgtacgtac ccgggtatgg cggaatttct caaagaggag ggaaaacgag
      301 aggtggacag tatcgtttgg gaggatgccg gaaaaaaaat ccgggccatc tacgacagcc
      361 tggtgtgaag acaagaagac agtaaaaatc taacagtcta aaaaaatatg aaaacaattt
      421 gcttttattt tcaaatccat caaccgtacc ggttaaaaag ataccgtttc ttcaatatcg
      481 gaagggacca ctattattac gatgattatt caaacgaaga catcatgcag cagatagctg
      541 cccggtcttt catcccggca aaccgcatgc tgctcgattt aataaacacc tataagggga
      601 agtttaaatt tgccatatcg gtttcaggag tagctcttga ccagatggaa gtgtatgctc
      661 cggaagtaat cgacggactg aaggaattaa gcagaaccgg cgctgttgag tttttagccg
      721 aaacatactc acactccctt tcaagcctga tcgatcctgt agaatttcaa agccaggtgc
      781 agcaacaaac gcagaagatc aaattacttt tcgatcagaa accaaaagtt ctgcgaaata
      841 cggaaatgat cttttcagac gatatcgctg aaatggctta caacatgggc tacactaaaa
      901 tggtttctga aggagctaaa ctcatcctct cctggagaag ccccaactat gtgtacaaat
      961 ccgaaaatca acccaacatg aaactattgc tgcgcaactc acagttcagc gacgacattt
     1021 cgctccgatt ttcagattat acgtggaaag aatatccgtt aacggcggaa aaatacatat
     1081 cgtggattgc tgcaacttca ccgcaggaag aggtattcaa tattttcatg aattatgaaa
     1141 cgttcgggaa cctccaaccg gctcattcgg gaatatttga atttatgaaa gcactgccta
     1201 aatttgcttt cgacaaaggc attgagtttt ccactcccag tcaaattatg gacacccata
     1261 aaccggtggg caccgtatcg gttcccctgg ctatttcctg gtcggacgaa gaacgcgatt
     1321 taagccggtg gctgggaaac aagcttcaaa aaagtgcttt caacaccctc tacgagatca
     1381 gtgaaagaat tcgcttggcc aacgacaggc gcttgaagaa agactggcaa tacctgcaat
     1441 cgagcgatca tttttattac atgtctacca agcattttta cgacggttca atgcacagta
     1501 aattcagccc ttaccaatcg ccctacgacg cgtttaacaa ttacatgaat gtcttaagcg
     1561 attttattga ccgggtcaaa gcacaattcc cggattcggt agacaatgaa gaattaaatt
     1621 cgcttctcac cactattcac aaccaggagc gtgaaattaa gaaactccaa tccgaactga
     1681 agaaagtact gacagagaat gaagaaaaat tagtggaaaa aactccggta aaataaatcg
     1741 gaccaggcta ttctctaccg gataaagcac aaagttaagg tttaaagatt agaaaccgtt
     1801 ttgaatttta tatttctatt tttttcgatt taatagttaa ctttgcacga tttttttaaa
     1861 agactgcaac atgatttatg caatgatcct ccttttcatc gcaggttatt tttttatcgc
     1921 gatggaacat accataaagg ttaacaaagc cagcgttgct ttactaatcg gtacgctttt
     1981 atgggtaatt tacgtgtatc tggcaccaag cacagtcctc agtgtctctc ccgatgcttt
     2041 caaccattat atccattcgc atcctgaact ccaacaccac gcttttggaa ttcaggtacg
     2101 ccactttgta attgatcatc agattctgga aagtattggg gacatcagtg aaatgttatt
     2161 ctttttgctt gccgctatga ccattgtgga gctcattgac gttcacggcg gattcacgta
     2221 tattactgaa aaaattacga cacgaaaaaa acgtcggtta ctctggttaa tcagctttat
     2281 cacgtttttt atgtctgccg ttctcgacaa cctgactaca accattgtaa tgattatgct
     2341 tacccgtcgt attatccgtg aaccccgtga acgatggctt ttcggcagta tcattgttat
     2401 tgcggctaac agcggcgggg cctggtctcc gatcggtgac gttacgacca tcatgttatg
     2461 ggttaaggga aacatcactg catccattat acctcagttg ttctttccaa gccttgtatc
     2521 catgatagcc ccggtttttg tagcacagtt tttattaaaa ggcaatatcc aggaagaaga
     2581 aatttcccaa acagaagaaa aagaaaactc cgaattattg cttcatatgc aaaaaaaaga
     2641 ccggctgggg atactgattt tcggggtttc catccttgtt ttatctcccg tgttcaaggg
     2701 agttacccac ctgccgcctt tcataggttt attactgggt gtaagcttat tatggattta
     2761 taccgagatt ctgtacaacc aaaaaaacca gctcaacgaa aacctgaagc ttcgtgtttc
     2821 aaaagtattg ggacgaatcg atttttctac cttaatgttt tttctgggaa tattgctggc
     2881 tgttgacgcc cttcaaagcg caggaattct tcaacaatct tccgccttta tggcacaaca
     2941 tcttcccaat gtctatgctc aggcctatgc cattggatta ttgtctgcca ttgttgataa
     3001 cgtccctttg gttgcagcag ctatcggtat gtacccggtg ctggatccgg ccgctttaag
     3061 tacgatggcc gatccggtgt ttatgcaaaa tttcgtggaa gacggtgttt tttggcattt
     3121 tctcgcctat tgcgcagggg ttggcggaag catccttatc attggttctg cggcaggtgt
     3181 ggtttttatg ggactggaaa aagtaccgtt tggctggtac ctgaaacgta tttcactcat
     3241 cgcacttatc ggatacacgt tcggggcagg ggc
//
LOCUS       DAOSDR010000358         3247 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrimonas sp. isolate AMR_MDS_1826
            NODE_10449_length_3247_cov_3.990915, whole genome shotgun sequence.
ACCESSION   DAOSDR010000358 DAOSDR010000000
VERSION     DAOSDR010000358.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863355
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrimonas sp. (wastewater metagenome)
  ORGANISM  Petrimonas sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Dysgonomonadaceae; Petrimonas.
REFERENCE   1  (bases 1 to 3247)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3247)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:10:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,720
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 35
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3247
                     /organism="Petrimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10449_length_3247_cov_3.990915"
                     /isolate="AMR_MDS_1826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2023866"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1687
                     /locus_tag="PLI30_11825"
     CDS             <1..1687
                     /locus_tag="PLI30_11825"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019541382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NAD(+) synthase"
                     /protein_id="HOI80277.1"
                     /translation="IKKKPSLIAIVGLPLLLKNSLYNVAAVISHEGVLGFVPKTYIPN
                     NNEFYEKRWFAGSRDLNDFFVEINGESIPVSSDGIIFHTPFASFGIEICEDLWMPVPP
                     SSKLAMQGADIIFNLSASNELVGKNAYRKSLVLQQSGRCNAAYVYASAGYGESSTDLV
                     FSGAAILAENATLLAEGKRFSPDNEMIISDIDVVALRGDRLKNSNFIPPQEESVSEIE
                     CALEAVSTGKWYRKFNPYPFIPSPEKEDEYLSEVFNIQTAGLAKRLQHTGIKTVTMGI
                     SGGLDSTLALLVTTKTFDFLGLPRENIIGITMPGFGTTGRTYNNAVELMKSVGVTVKE
                     IPIVNAVTQHLKDIEHDINLHDITYENAQARERTQILMDYANKINGLVVGTGNLSELA
                     LGWATYNGDHMSMYGVNASVPKTLVKTLVRWIADTQMGDQPRKILHDILDTPFSPELL
                     PADKEGKIAQKTEHFIGPYELHDFFLHRMLRYGDSPKRIYFIAQQAFVNQYSPEEILK
                     WLKVFYKRFFAQQFKRSCMPDGPKVGSVNLSPRGDWRMPSDAVVNVWLKELEN"
     gene            1692..3119
                     /locus_tag="PLI30_11830"
     CDS             1692..3119
                     /locus_tag="PLI30_11830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018712075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HOI80278.1"
                     /translation="MRMKELVQLFKNYTGKSAPKIDPLPSSGSNRRYYRLRQDEVSVV
                     GVYGQSKEENHAFIELSRHFHKQRLNTPRVLAVSGDEMFYIQEDLGDSVLFDALKAGR
                     LTGVFSTEEKEILHKTIALLADFQVLGAKDLDFSVCYPQAEFNHRSVHWDLNYFKYNF
                     LKTTGMEFQEDLLENDFDKLSQHLLQDESDTFLYRDFQSRNVMLVDGRPYFIDYQGGR
                     KGPVHYDVASFLWQAKANFPDDLRDELIKTYIASLKKYREVDEAEFIEKLRLFVLFRT
                     LQVLGAYGFRGYFEKKPHFIQSIPFALNNLRELLKGGFNEYPYLTGMLNEMVGLKQFA
                     DTQKRELEVRVFSFAYKKGIPNDVSGNGGGYVFDCRAINNPGKYERFSHFTGLDEEVI
                     KFLEEDGEMELFLDNIYPLVDSHVKRYMERNFTSLMVSFGCTGGQHRSVYAAQRMAEH
                     ISKKFGIKVSLVHREQNLEQEFRKK"
     gene            3158..>3247
                     /locus_tag="PLI30_11835"
     CDS             3158..>3247
                     /locus_tag="PLI30_11835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004300821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HOI80279.1"
                     /translation="MKAMIFAAGLGTRLKPLTDTMPKALVPVAG"
BASE COUNT          904 a          790 c          796 g          757 t
ORIGIN      
        1 cataaagaag aagccttcgc tcatcgccat tgtgggactt ccgttgctgc taaaaaacag
       61 cttgtacaat gtggctgcgg ttatttcaca cgaaggggta ttgggttttg tccccaaaac
      121 gtatattccc aacaacaacg agttttacga gaaacgatgg tttgcgggaa gccgtgacct
      181 gaacgatttt tttgtcgaga tcaatgggga aagcatacct gtctcttccg acgggataat
      241 ttttcacacg ccgttcgcgt ctttcggaat cgagatctgt gaagaccttt ggatgccagt
      301 gcccccttct tcgaaactag cgatgcaggg tgccgacatc atcttcaacc tttccgccag
      361 caacgagctg gtaggcaaaa acgcgtaccg gaaatcattg gttttacagc agtcggggcg
      421 ttgcaatgcc gcgtatgtct atgcttctgc cggatacgga gagtcatcca ccgatctggt
      481 tttttccggt gcggcaatcc ttgccgaaaa cgccacgctg cttgccgaag ggaaacgttt
      541 ctccccagat aacgagatga tcatttccga catcgatgtc gtggctttac gaggcgaccg
      601 gctaaagaac agcaatttca tccctccaca ggaggagtct gtttcagaga ttgaatgtgc
      661 gctggaagcg gtttctacgg gaaaatggta ccggaagttc aacccgtatc ccttcatccc
      721 gtcaccggaa aaagaagatg agtatttaag cgaagttttc aacatacaaa cggcggggct
      781 agcaaaacga ttacaacaca caggaatcaa gacagtaact atgggtattt cgggcggact
      841 ggattctacg ctggcactgc tggttactac aaaaaccttt gattttctcg gacttccacg
      901 cgaaaatata atcgggatca ccatgcccgg atttggaaca accggcagaa cttataataa
      961 tgctgtggaa ttaatgaaat cagttggagt aacggtaaaa gaaatcccga ttgtcaatgc
     1021 cgttacccaa cacctgaaag atattgaaca cgatatcaac cttcacgata tcacctacga
     1081 aaatgcccag gcgcgcgaac gaacacaaat tttgatggat tatgccaata aaatcaatgg
     1141 tttggtggtg ggaaccggaa atttatcgga actggcgttg ggttgggcaa cctacaacgg
     1201 cgaccacatg tcgatgtacg gagtaaacgc aagcgttcca aaaacattgg ttaagacgct
     1261 ggtacgctgg attgccgaca cgcagatggg cgaccaaccc cggaaaattc ttcatgacat
     1321 cctcgacacg cccttcagtc cggaactgct tccggcagac aaagagggga aaattgccca
     1381 aaagaccgag catttcatcg gcccatacga actacacgac tttttcctgc accggatgct
     1441 tcgttacgga gattccccca aacgaatcta tttcatcgca caacaggcat tcgtcaatca
     1501 atattccccg gaagagatcc tgaaatggct gaaagttttc tataaacgtt tttttgccca
     1561 gcaattcaag cgctcctgca tgcccgacgg ccccaaagtg ggctccgtca atctctctcc
     1621 ccgcggcgac tggcgtatgc ctagcgatgc cgtggtaaat gtatggttaa aagaactgga
     1681 gaattaagac catccgcatg aaagaattag tacaattatt taagaactat accggaaagt
     1741 cggcccccaa aatagaccct ctgccttcgt caggatccaa ccgcaggtat taccggttaa
     1801 ggcaggacga agtatctgtt gtaggggtat acggacaatc caaagaggag aatcacgctt
     1861 ttatcgaact ctcgcgccat tttcacaaac agcggctaaa taccccccgg gtgctcgctg
     1921 tttccggtga tgaaatgttt tacatccagg aagatttggg agacagcgta cttttcgatg
     1981 cgctgaaagc cgggcgactg acgggcgtgt tcagtacgga agaaaaggag atactgcaca
     2041 agaccattgc cctgttggcc gattttcagg tgctgggggc taaagatctc gatttcagcg
     2101 tgtgttatcc tcaggccgaa ttcaatcacc gttcggtaca ctgggattta aattacttca
     2161 agtacaactt cctaaaaacc accggtatgg aattccagga agatcttctg gaaaacgatt
     2221 ttgataaact atcccaacac ctgcttcagg acgagtcgga cacgtttttg taccgtgact
     2281 tccagtcgcg caacgtgatg cttgttgacg gccggcctta cttcatagac taccagggag
     2341 ggagaaaagg tccagtgcac tatgatgtgg cttcgttttt gtggcaggca aaagccaatt
     2401 tcccggatga cctgcgcgat gaactcatca agacatacat cgcttcactc aaaaagtaca
     2461 gggaggtaga cgaagccgaa ttcatagaaa agctccggct gttcgtactg ttccgcacgt
     2521 tgcaggtcct tggcgcctat ggattcaggg ggtatttcga aaagaagcca cactttatcc
     2581 agagtattcc gttcgcgctc aacaaccttc gggagctact caagggtggt ttcaacgaat
     2641 atccctatct gaccggaatg ctgaacgaga tggtcggcct gaaacagttt gccgacactc
     2701 aaaaacgcga actcgaggta cgggtattca gcttcgcata caaaaaagga atccccaacg
     2761 atgtatcggg aaacggcggc gggtatgtgt tcgattgccg tgcgataaac aatcccggga
     2821 agtacgaacg gttcagccat ttcacgggac tggacgaaga ggtcattaag tttctggagg
     2881 aggatggcga aatggaactg tttttagaca acatttaccc gctggtcgac agccacgtga
     2941 aacgatatat ggaaaggaac ttcactagcc tcatggtctc gttcggctgc acaggcggac
     3001 aacaccggtc ggtttacgcc gcacagagaa tggcggaaca tatctcaaaa aaattcggaa
     3061 tcaaagtgtc tctcgttcac cgggagcaga acctggaaca ggagtttaga aagaaatagc
     3121 ctaataaact caagccctaa aaaccagaaa cctctcaatg aaagcaatga ttttcgctgc
     3181 cggactcggc acgaggctta agcccctgac ggataccatg cccaaagcgt tggttcccgt
     3241 ggcggga
//
LOCUS       DAOSDR010000359         3244 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrimonas sp. isolate AMR_MDS_1826
            NODE_10458_length_3244_cov_4.304798, whole genome shotgun sequence.
ACCESSION   DAOSDR010000359 DAOSDR010000000
VERSION     DAOSDR010000359.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863355
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrimonas sp. (wastewater metagenome)
  ORGANISM  Petrimonas sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Dysgonomonadaceae; Petrimonas.
REFERENCE   1  (bases 1 to 3244)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3244)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:10:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,720
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 35
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3244
                     /organism="Petrimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10458_length_3244_cov_4.304798"
                     /isolate="AMR_MDS_1826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2023866"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1239)
                     /locus_tag="PLI30_11840"
     CDS             complement(<1..1239)
                     /locus_tag="PLI30_11840"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HOI80280.1"
                     /translation="MRLKMKVTRLFALMFVLLISSVTSAQPLTKVFSNETLKNVLKEI
                     ENQTGLSVIYRNSDLNESKRITASFDDASLSEVMNAILDGNLQYSIQNNILVISRKET
                     TPVTEVEKVQQGKTIKGKVTDATGEPIIGATLTVAGTTKGTATDYNGNYIIEGVSDSD
                     VISVSFIGYISQELSATSPQLSNIVLQEDNKLLEEVVVTALGIKREEKSLGYAVQNVS
                     GNSLQTIKGVDMTASLTGKVAGLVIKNSTEFNGTSGIEMRGESPLLVINGVPYGNMSL
                     RDIPADEIENITTLKGATAAALYGARGSAGAIMITTKRGANKGLSIDVNSNTMFRAGW
                     VAIPKAQTSYGHGLNGELADDYVWGPKLDIGNTALQWNPTTKQNEVMPLVSSGKNNFR
                     NFLEPGLITNNSINVTQTGEN"
     gene            complement(1247..2230)
                     /locus_tag="PLI30_11845"
     CDS             complement(1247..2230)
                     /locus_tag="PLI30_11845"
                     /inference="COORDINATES: protein motif:HMM:NF016648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR domain-containing protein"
                     /protein_id="HOI80281.1"
                     /translation="MEEQQQHIKLLAFRYFEGKSSREDAEKIFAFLKEDDSHHSIFRK
                     WENDWINSLELHPELNERWEQIKLANRNTEFIKKSVPVIKPYRNYIYAVASIAVLILG
                     VFFALMSMQDLQPEEFFILETPLGQKSKITMPDGSNVWLNSGSSLIYSNKYGKKNREV
                     QLSGEAYFEVRKGLKDFIVRINEYSVIVKGTRFNVSAYRDDQLLTTTLLEGSVEFNYI
                     QGSLEMNPGETMEMNLETNSITCKKSQHSTNAWTRNRLEYENIELEELLKKLSREYAR
                     PIFLESDSLSKQSFSISIDTNKDLSDVLNAIAEIIPIKTIHQNDTIKLKPI"
     gene            complement(2320..2940)
                     /locus_tag="PLI30_11850"
     CDS             complement(2320..2940)
                     /locus_tag="PLI30_11850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009040048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma-70 factor"
                     /protein_id="HOI80282.1"
                     /translation="MVDFSSNYDLFNEVKKGNEKAFEYVFKRFYFRLKGFALRFVGDP
                     DVVEDIIQECFLTLWEKRERLALISLPSLLFIMVRNSCLNHLKHVSVKNKLISGIHPL
                     TEIEERLYYADFELDASQKLLYDELDEQVNKVIEVLPDRCKEVFVLSRNEGLKNREIA
                     EKLKISTTAVEKHISKALSIFSFHLKEKYPVDYTFFFLVFSPMLFA"
BASE COUNT          891 a          661 c          641 g         1051 t
ORIGIN      
        1 gttttccccg gtttgtgtta cattaatgct gttgtttgtt atcaatccgg gctccagaaa
       61 attgcgaaaa ttatttttcc cgctcgaaac cagtggcatc acttcgtttt gtttggtagt
      121 cggattccat tgaagagctg tattgcctat atcaagttta ggtccccaga cataatcgtc
      181 ggccaattct ccattcaaac catggccgta tgaggtttgt gccttaggta tggctaccca
      241 accggccctg aacatcgtat tgctgttgac atcaatagac aaccctttgt ttgctccacg
      301 ctttgtggtt atcataattg ctcctgccga gcctctcgca ccgtacaatg cggcagccgt
      361 ggcccctttc agagtggtta tattttcaat ttcgtcggcc ggtatgtcac gaagcgacat
      421 gttcccgtag ggcacgccat tgataactaa aagcggagat tccccacgca tctctattcc
      481 cgatgttcca ttaaattcag ttgagttctt aattacaagt ccggcaactt ttccggtcag
      541 tgaagctgtc atatctaccc ctttgatggt ttgcaaggag tttccggaga cattttgcac
      601 ggcataaccc agtgattttt cttcccgctt tattcccaaa gccgtaacca caacttcttc
      661 cagtaatttg ttgtcttcct gtaaaacaat attggagagt tgggggcttg tggcagaaag
      721 ttcctgtgaa atataaccga tgaacgatac ggaaatcaca tcggaatcgg aaacaccttc
      781 aataatgtag tttccgttat aatccgttgc agttcctttt gtagtccctg ccacggttaa
      841 ggtggctccg ataatgggtt ctcccgttgc atccgtaact ttccccttaa tggtttttcc
      901 ttgttggacc ttttcaactt ccgttactgg tgttgtttct ttcctggaaa taacaagaat
      961 attgttttga atgctgtatt gcaaattgcc gtccaatatg gcgttcatta cttcagaaag
     1021 agaggcatcg tcgaacgatg cggtgattct cttgctttca ttcaaatcgc tgtttctgta
     1081 aataacagac aggccggttt gattttcaat ttcttttaaa acatttttca acgtttcgtt
     1141 gctgaaaact ttcgttaatg gctgtgcact cgtcacggag ctgataagca gaacaaacat
     1201 gagtgcaaac aaacgggtta ctttcatttt taatctcata attagtttaa atgggtttta
     1261 atttaattgt atcgttctga tgaatcgttt ttataggtat tatttcggcg atagcgttca
     1321 acacatccga taaatctttg tttgtatcga tactgataga aaaggactgt ttgcttagcg
     1381 agtcgctctc cagaaatatt ggccttgcgt attccctcga taatttctta agcagttctt
     1441 caagctctat gttttcatac tccaggcgat tccgggtcca ggcattggtg gagtgctgac
     1501 tttttttgca tgtgatagaa ttggtttcaa gattcatttc cattgtttcg ccgggattca
     1561 tttccagtga gccttgtatg tagttaaatt ccacactccc ttccagtaac gttgtagtaa
     1621 gaagttggtc gtcgcggtag gcagatacgt tgaaccgggt accttttaca atgacgctgt
     1681 attcattaat tcgtacaata aaatccttga gcccttttct tacttcgaaa taagcttcac
     1741 ccgataattg aacttcgcgg tttttctttc cgtacttatt ggaatagatt agcgaagatc
     1801 cagaattcaa ccacacatta ctgccatctg gcatggtgat tttacttttt tggcccagcg
     1861 gagtttccag gatgaaaaat tcttcgggct gaagatcctg catgctcatg agcgcgaaaa
     1921 aaacgccgag gataagaact gcaattgagg caaccgcata aatataattc cggtagggtt
     1981 ttatgacggg tacggatttc ttgataaatt ccgtatttct gttcgccagt tttatttgtt
     2041 cccacctctc gttcaattcc gggtgaagct ccagggagtt gatccagtcg ttttcccatt
     2101 ttctgaaaat cgagtgatgg gaatcatctt ctttcaggaa agcaaatatt ttttcggcat
     2161 cctcccggga gctttttcct tcgaaatacc gaaaagccaa taattttata tgttgctgtt
     2221 gttcttccat gttgtcctaa agatataaca accggatagt attttaccca acctcccaca
     2281 atgttaaaac ttttttttaa agaataacaa gaaagaatgt caggcaaaca gcatagggga
     2341 aaaaaccaga aaaaagaagg tgtagtctac ggggtatttc tctttcaaat ggaacgaaaa
     2401 aatactcaat gccttgctaa tgtgtttctc tacggctgtg gtggatattt ttaatttttc
     2461 ggcaatctcg cggtttttca ggccctcatt ccggcttaat acaaacactt ccttacatcg
     2521 atcggggagt acttctatta ccttattcac ttgttcgtcc agttcatcgt aaagtagttt
     2581 ctgagaagca tcaagttcaa aatcagcgta atagagccgt tcttcgattt ccgttaaagg
     2641 gtgaatgcct gagatgagtt tgtttttcac cgaaacgtgt tttaaatggt tcaggcaact
     2701 gtttctcacc ataatgaaaa gaagcgaagg aagggatatc aaagccagcc tttctctttt
     2761 ttcccataac gtaagaaaac actcctgaat aatgtcctca accacgtcgg gatcaccaac
     2821 aaaacggagg gcaaaccctt ttaaacgaaa ataaaaacgt ttaaagacat attcaaatgc
     2881 cttttcgttg ccctttttaa cttcgttgaa taaatcgtag ttgctggaga aatctaccat
     2941 aaaagtaaaa aactatgaat tctttacaaa gaaataaaaa aatgttgtaa agtgaggaat
     3001 atgacaattt ttttaataaa attataaaaa aacgtaggtg taacgtgccg gaattatttt
     3061 aatgtatggc ctttgcctgg aaggaacaat cgattgttct atggatttga aaattgcttg
     3121 tgatttggtg ctttatcccg aacaaaaaat gtagatttgc gtttgaatta aaagtataat
     3181 caaaaccaaa aatacgttcg gagcaaaccg acacgtaaaa agaattatcg tatgaaatgg
     3241 ataa
//
LOCUS       DAOSDR010000360         3235 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrimonas sp. isolate AMR_MDS_1826
            NODE_10499_length_3235_cov_4.596855, whole genome shotgun sequence.
ACCESSION   DAOSDR010000360 DAOSDR010000000
VERSION     DAOSDR010000360.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863355
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrimonas sp. (wastewater metagenome)
  ORGANISM  Petrimonas sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Dysgonomonadaceae; Petrimonas.
REFERENCE   1  (bases 1 to 3235)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3235)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:10:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,720
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 35
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3235
                     /organism="Petrimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10499_length_3235_cov_4.596855"
                     /isolate="AMR_MDS_1826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2023866"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..352)
                     /locus_tag="PLI30_11855"
     CDS             complement(<1..352)
                     /locus_tag="PLI30_11855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019539929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family membrane protein"
                     /protein_id="HOI80283.1"
                     /translation="MKFLTELSRIILGITFAFSGFVKAVDPLGFTYKIQDYLVSFDLP
                     GLFPLALPAAITLVVLEFSLGVFLLLGIYRKPTVRLAAVFMAIFLPLTLWIALKNPVK
                     DCGCFGDALIISNWE"
     gene            complement(413..958)
                     /locus_tag="PLI30_11860"
     CDS             complement(413..958)
                     /locus_tag="PLI30_11860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007655754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1599 domain-containing protein"
                     /protein_id="HOI80284.1"
                     /translation="MSETNRQFDEVIAICRNMFEKKSSDYGPTWRILRPESVTDQLLI
                     KANRIRSLEIKKESKVGEGIFPEFIGIVNYGIMGLIQLELGYADSVDITNETALQLFD
                     KYITAAKELMYAKNYDYDEAWRSMRVSSYTDLILAKLFRIKEMENKQGKTIVSEGIDA
                     NYTDIVNYALFGLIKLHFGEE"
     gene            complement(1069..2082)
                     /locus_tag="PLI30_11865"
     CDS             complement(1069..2082)
                     /locus_tag="PLI30_11865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019539931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HOI80285.1"
                     /translation="MERDKNLSKVKVLFSVSLVFFTLSAFAQSKVELSAQSRPEVNYK
                     QLHSASLGNQEGLFADGIKLKMDLSVLEEAEAAREAFDVNEIPCDDIYGGVWKNAHVD
                     IYGALKNAPDSFIVNLTNFSMPHVGRMTSNFGRRGSRRYHYGIDLKLQVGDTIYAAFD
                     GKVRVQQYERRGYGYYAVIRHVNGLETVYGHLSKFLVKENDFVKSGQPIALGGNTGRS
                     TGPHLHFETRFLGKPINPNYLIDFNNKVCHRDEYLVTNSSYRKTSQSSRVLTNASNYK
                     EPTSTANNKYVSGTVKYHRIKSGDTLGAIARRHGLSVSRLCSMNNITPKTTLRIGKSL
                     RVS"
     gene            complement(2137..2598)
                     /gene="ndk"
                     /locus_tag="PLI30_11870"
     CDS             complement(2137..2598)
                     /gene="ndk"
                     /locus_tag="PLI30_11870"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006801340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0006165 - nucleoside diphosphate
                     phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HOI80286.1"
                     /translation="MEKTLVILKPSAVQRSIVGEVVSRFEKKGLQIVGMKMVSLSDSI
                     LEEHYSHLKEKPFFKRIKDSMQVSPVIVMALEGLEAVEVVRMMAGPTNGRKAQPGTIR
                     GDYSVSVQENIIHASDSPETAEAELKRFFSENEIFNYPSPAFVNLYANDEV"
     gene            3024..>3235
                     /locus_tag="PLI30_11875"
     CDS             3024..>3235
                     /locus_tag="PLI30_11875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008773141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2723 domain-containing protein"
                     /protein_id="HOI80287.1"
                     /translation="MKKYKLINTISGWVVFVIAAVVYLMTIEPTASFWDCGEFISSAY
                     KLEVGHPPGAPIFMLLGNLFTQFTNDP"
BASE COUNT          892 a          772 c          601 g          970 t
ORIGIN      
        1 tctcccaatt actgatgatt agcgcatcgc caaaacaacc gcaatccttg accggatttt
       61 tcaacgcaat ccagagcgtg agcggaagga agatagccat aaaaacagcc gcgagccgga
      121 cagtgggctt gcgatagatc cccagcagca aaaaaacacc cagcgaaaac tcaaggacaa
      181 ccaaggtaat ggctgccggc agcgccaggg gaaacaggcc cgggaggtca aaagaaacaa
      241 gataatcctg aatcttatag gtgaaaccca acgggtcaac ggctttcaca aatcccgaaa
      301 aagcaaaggt aatcccaaga ataatccggg acaattccgt cagaaatttc acaagctttt
      361 cacgggaatt catcgttgag cctctgtact gattaatgat aattataaaa aactattctt
      421 ccccgaaatg gagttttatc aatccaaaca aggcatagtt cacgatatcc gtatagttag
      481 catcaatacc ttcagacaca atggttttcc cttgcttgtt ctccatctct ttgatccgga
      541 acagtttcgc cagtatcaag tcggtgtatg agctgacacg catactgcgc caagcttcat
      601 cgtaatcgta gttcttggcg tacatcaact ccttagctgc ggtgatgtat ttatcgaaaa
      661 gctgcagtgc tgtttcgttg gtaatgtcca cactgtcggc ataccccagc tcgagctgta
      721 tcaaccccat aatgccgtaa ttgactattc cgatgaattc cggaaagata ccctcgccca
      781 ccttgctttc cttcttgatc tccagtgaac gaatccggtt ggctttaatc aacaactgat
      841 ccgttaccga ttcaggacgt aaaatccgcc atgtaggccc gtaatcactc gacttcttct
      901 caaacatgtt tctgcaaatg gcgatcacct catcgaattg ccggtttgtt tcactcatag
      961 gaatagcact gtttttttat ctttaatgaa gccacaaagt taaacaaaaa aagaaaaagg
     1021 tacaagctca acctcctgca cctttccctc ttaattagaa attctacttt atgaaactct
     1081 taacgatttc cctatccgca gcgtggtctt cggggtaatg ttgttcatgc tgcacaacct
     1141 cgatacggat aagccgtgtc ttctggcgat agctcccagc gtatctccgc ttttaatccg
     1201 gtgatatttc actgttcccg aaacatattt gttgttggcg gtagaggtgg gttctttata
     1261 attgctggca ttggtaagca ccctgctgct ttgggatgtt tttctgtagc tcgaattggt
     1321 aaccaggtac tcgtcgcgat ggcacacctt gttgttgaag tcgatcaggt aattcggatt
     1381 gatgggtttt cccaggaacc gggtctcgaa gtgcaggtgc ggaccggtag accttccggt
     1441 gttcccgccc aaagcgatgg gttgccccga cttgacaaag tcgttttcct tcaccaaaaa
     1501 cttcgataaa tgtccgtaaa ccgtttccaa tccgtttacg tgacgtatta ccgcataata
     1561 tccataccct ctccgttcat attgctgcac cctgaccttt ccgtcgaaag cagcgtagat
     1621 ggtgtcgccc acctgcaatt ttaaatcgat accgtaatgg tagcggcggc tcccccttct
     1681 tccgaagttt gaagtcattc ttcccacgtg aggcatggaa aagttagtca ggttgacgat
     1741 aaaagaatcg ggagcatttt ttaaagcacc gtaaatatcc acatgggcat ttttccacac
     1801 tcccccgtaa atatcgtcac acggaatttc gtttacatcg aatgcttcac gagccgcttc
     1861 ggcttcctca aggacagaca agtccatttt gagctttata ccgtcggcaa ataatccttc
     1921 ctgatttcct aaacttgcac tgtgtaattg tttgtaatta acttccggtc tggattgtgc
     1981 ggataactcc acttttgatt gagcgaacgc gctcaacgta aaaaatacca aagagacgga
     2041 aaacaatact tttaccttag ataaattctt atctctctcc ataataagat atagctgaga
     2101 atggccttta gttacaactt aattaattta ctgatgtcag acttcatcgt tagcgtaaag
     2161 attaacgaaa gccggtgatg gatagttaaa aatctcgttt tccgaaaaaa agcgtttcaa
     2221 ttctgcctct gccgtctccg gagaatcaga tgcatgaata atattctcct gcacactgac
     2281 gctgtagtct ccccgaatgg ttcctggctg agcttttctt ccgtttgtcg gccctgccat
     2341 catcctcacc acttcaaccg cttctaaacc ttccaacgcc ataacaatga ccggacttac
     2401 ctgcattgaa tctttgatgc gtttgaagaa tggcttttcc tttaagtgag aataatgttc
     2461 ttccaatatt gagtcagaca aagacaccat cttcattccc acaatttgaa gccctttttt
     2521 ttcaaaccgc gaaacaactt cacctacaat gctccgttgt accgccgaag gcttaagaat
     2581 tactaatgtt ttctccatta aatctgacaa taaaaaaaga aaatgacctt tgaataacga
     2641 tttttttcct taatccttac caaagtaaat tgtttccttt tttttgacca aattatcttt
     2701 caataaattt tggcactcaa accctaacaa agttaaattt ttgttaacaa aatgacgcaa
     2761 atatctcatt ttctgttatt taaaatgaaa catgttttaa aacataaatc gccgctttta
     2821 acttttgcat agttcagtta acatttgttt gagtgcaatt gattatttac gacaaggata
     2881 acttttcgcc caccctccat tgttttttgg taagcaactc attgacactg catcgctttt
     2941 tttctttttt taataagatc tgctaattgt ttttcttacc tttgtccaaa ttttcggttg
     3001 agtatcattt ttatcacgaa ataatgaaaa aatacaaact tatcaataca atttcaggtt
     3061 gggttgtttt tgtaattgcc gctgttgttt atctgatgac cattgaacca acggccagtt
     3121 tctgggactg cggcgaattt atctcatcgg cctacaaact ggaggtaggg catcctcccg
     3181 gcgcacccat ttttatgctt ctcgggaact tgttcacaca attcaccaac gatcc
//
LOCUS       DAOSDR010000361         3209 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrimonas sp. isolate AMR_MDS_1826
            NODE_10614_length_3209_cov_5.247939, whole genome shotgun sequence.
ACCESSION   DAOSDR010000361 DAOSDR010000000
VERSION     DAOSDR010000361.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863355
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrimonas sp. (wastewater metagenome)
  ORGANISM  Petrimonas sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Dysgonomonadaceae; Petrimonas.
REFERENCE   1  (bases 1 to 3209)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3209)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:10:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,720
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 35
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3209
                     /organism="Petrimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10614_length_3209_cov_5.247939"
                     /isolate="AMR_MDS_1826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2023866"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(108..533)
                     /locus_tag="PLI30_11880"
     CDS             complement(108..533)
                     /locus_tag="PLI30_11880"
                     /inference="COORDINATES: protein motif:HMM:NF016087.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper resistance protein NlpE"
                     /protein_id="HOI80288.1"
                     /translation="MMKKLSILLFAFVAIVFISCNNAKQKNAETPDMHTSEMALDYLG
                     VYEGILPCADCEGIKTQLTIHDDNTFTLVSDYLGEKEAKFEDKGSYFIESGEILVIQD
                     EDGDQKYFKLQEGSLAQLNADKKMIEGELAPFYVLTKVR"
     gene            complement(606..2663)
                     /locus_tag="PLI30_11885"
     CDS             complement(606..2663)
                     /locus_tag="PLI30_11885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019540110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI80289.1"
                     /translation="MKPLLSVSLLLALLVSCNTHPSKKENDMSESRFIAEAQRNEVIS
                     MLKDSLGEPAYFRIERGVRQVADLWREPDGTAGEFAEFCKRSFVADEARLDQLFHTLE
                     RNFEVFDGYFHKIDVLLKEPLQLEGEEITPVDMMFGSYDVTAHLNDDLYANKIAFITA
                     LNFPFYSLQEKTGLGEKWSRKEWAYARMGDRFTARVPATVQQNISKTITEADAYISDY
                     NLFMGKLRNDNGEQLFPDDMKLISHWGLRDELKSNYADTVRGLEKQRMIYTVMKRIID
                     QTIPQQTINSDKFTWNPGTNQLFENNAEASASPEPDTRYKIFLANFHAMRQLDTYFPH
                     YPTQLSRAFDATMEIPQKDVETLFKGLLSSPEVKEVAAFIESRLGRKLEPFDIWYNGF
                     KARGGIPEEQLTAITSKKYPNPKALETDLPNILVKLGWKPDRAREISSLIVVDPSRGA
                     GHAWGATMRNDIAHLRTRIGAGGMDYKGYNIAVHEFGHTVEQTITMNDVDYYVLNGVP
                     NTAFTEAVAFLFQKRDLELLGLKNPNPEDEHYLALDNFWSSYEIMGVSLVDIRVWEWL
                     YNHPDATPDQLKEAVITAARDVWDTYYADILGGKGETLLGIYSHMIDYPLYLPNYPMG
                     HLIDFQIGQQVRGKNLADEMDRMYTQGRIIPQLWMKNAVGSGISIEPLLASTKAALYA
                     LKK"
     gene            2781..>3209
                     /locus_tag="PLI30_11890"
     CDS             2781..>3209
                     /locus_tag="PLI30_11890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019540108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HOI80290.1"
                     /translation="MQEINNYVETHKQRFLDELFELIRIPSISSLPEHKPDMYRAAEK
                     WKEILLAAGADKAEVYETDGNPVTYGEKIIDPKAPTVMVYAHMDVMPVDPVGLWKTDP
                     FEPVVKEGKIWARGADDDKGQGFMHAKAFEYLVKSEKLNCN"
BASE COUNT          815 a          745 c          726 g          923 t
ORIGIN      
        1 gaacccttgg tacccgtatg agtacaacgg ttttcgagac cgccccgatc gaccactccg
       61 gcatctttcc ttttaaagcc tattcgctat tggctttatg tcaagtctta ccttactttt
      121 gtaagaacgt aaaaaggagc caattctcct tcaatcattt ttttatcggc attcaactga
      181 gccagacttc cttcttgcag tttaaaatat ttttgatctc cgtcttcgtc ctgaataact
      241 aaaatttctc cactttcgat aaaataggag cctttatctt caaacttagc ttccttttca
      301 cccaggtaat cgctgacgag agtgaatgtg ttgtcgtcgt ggattgtcag ttgggtttta
      361 atgccctcac aatcagcaca aggaagtatg ccttcgtaaa cgccaagata gtctaaagcc
      421 atttcagatg tatgcatatc gggagtctcg gcattttttt gttttgcgtt attgcagctg
      481 atgaagacaa ttgcaacaaa agcaaataat aaaatactta actttttcat cattaaaata
      541 tggttttaaa aaacgataca aaggtacaat atttttggat atttgcatgt tgtggcccct
      601 gaaagttatt tctttaaagc atataacgct gcttttgtgg atgccagaag cggctcaata
      661 gatatgccgg agccaacagc gttcttcatc cacagctgtg gaatgattct gccctgggtg
      721 tacatccggt ccatttcgtc ggcaagattt tttccgcgca cctgttggcc gatctgaaaa
      781 tcaatgaggt gtcccatcgg atagttcggt agataaagcg ggtagtcgat catgtgcgag
      841 taaatcccga gaagcgtctc tcccttccct cccaaaatat cggcataata ggtgtcccac
      901 acatctcttg cagccgttat aaccgcttct ttcaattgat cgggagtggc atccggatgg
      961 ttgtaaagcc attcccaaac gcggatatcc accagggaaa ctcccatgat ctcgtaactc
     1021 gaccagaagt tgtccaaagc caggtagtgc tcgtcttcag gatttggatt tttgaggccc
     1081 agcagttcca agtctctttt ctggaaaagg aaagccactg cttcggtaaa tgctgtgttg
     1141 ggtacaccgt tgagaacata gtagtctaca tcattcatcg taatggtttg ctccaccgta
     1201 tgaccgaact cgtgtacggc aatattatag cctttataat ccattccgcc agcaccgatt
     1261 cttgtacgca gatgagctat gtcgttacgc atcgttgccc cccaggcatg cccggcgcca
     1321 cgtgacgggt caaccacaat gagtgatgaa atttcccggg ccctgtctgg cttccatccc
     1381 agtttaacca ggatattggg gagatcggtc tccaatgctt ttgggttcgg gtatttttta
     1441 gaggtgatgg ctgtaagctg ttcctcgggt attcccccgc gggctttaaa tccgttgtac
     1501 caaatatcga acggctccaa tttccgtcct aagcgcgact cgataaatgc ggctacttct
     1561 tttacttcgg gtgaagagag caacccttta aaaagggttt ccacgtcttt ttgcggaatc
     1621 tccatggtcg catcaaaggc tcgcgagagt tgagtgggat aatgcggaaa ataggtgtcg
     1681 agctggcgca tagcgtggaa attggcgagg aaaatcttgt accgggtatc gggttcggga
     1741 gaagctgatg cctcggcatt gttttcaaac aattgattcg tacccgggtt ccacgtgaat
     1801 ttgtcgctgt taatggtttg ctgaggaata gtctggtcga tgatacgttt catcaccgtg
     1861 tatatcatgc gttgtttttc tagccccctg actgtatcgg catagttcga tttcagttca
     1921 tcgcgtaacc cccaatgtga aatcagtttc atatcgtccg ggaaaagctg ttcaccgtta
     1981 tcgttgcgaa gctttcccat aaaaagattg tagtcggaaa tgtatgcgtc ggcctcggta
     2041 attgttttcg atatgttttg ctgaacagtt gccggcacac gcgcggtgaa acggtcgccc
     2101 atgcgggcgt aggcccattc ttttcttgac catttctccc ccagccctgt cttctcctgt
     2161 aacgaataga atgggaagtt aagtgctgta ataaaggcaa ttttattggc atataggtca
     2221 tcgttcaggt gtgctgttac atcgtagctt ccaaacatca tgtctacggg ggtaatttct
     2281 tcaccttcca gttgtagcgg ctctttcaac aggacatcga ttttgtggaa gtatccgtcg
     2341 aatacttcaa agtttcgctc cagtgtatga aaaagctgat cgagtcgagc ttcatcagca
     2401 acaaaactcc gcttgcagaa ctcggcaaat tctcctgccg ttccgtccgg ttcccgccaa
     2461 aggtcagcaa cctgccggac accgcgttca atacggaaat aggccggttc tcccaacgaa
     2521 tccttcaaca ttgaaattac ttcgttgcgc tgcgcttctg caataaaacg gctttctgac
     2581 atatcattct ctttttttga tgggtgggta ttacaagaaa caagtaatgc caacagtagt
     2641 gaaacggata gtagtggctt catctttttc atgtttaaac tttaacatta tgttgcaaag
     2701 gtaattaaaa agcgcttcat ccgcttctgg atgcaatttt ttttgtagtt ttacgcccaa
     2761 accctaaaac taaattaatt atgcaggaga ttaacaacta cgtggaaacc cataagcaac
     2821 gatttttaga cgagctcttc gaactgatcc gaatcccatc catctcatca cttcccgagc
     2881 acaaaccgga catgtaccgc gccgccgaaa aatggaaaga aatcctgctt gctgccggtg
     2941 ctgataaggc ggaggtatat gaaaccgatg gaaatccggt gacatatggc gagaagataa
     3001 tcgaccccaa ggcacccacc gtaatggttt acgctcatat ggacgttatg ccggttgatc
     3061 cggtcggctt atggaaaacc gacccctttg aaccggtggt gaaagagggg aagatctggg
     3121 cgcgtggcgc cgatgacgat aaaggccagg gatttatgca cgccaaagcg tttgaatacc
     3181 tcgtgaagtc ggaaaaattg aattgtaac
//
LOCUS       DAOSDR010000362         3203 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrimonas sp. isolate AMR_MDS_1826
            NODE_10651_length_3203_cov_3.636595, whole genome shotgun sequence.
ACCESSION   DAOSDR010000362 DAOSDR010000000
VERSION     DAOSDR010000362.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863355
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrimonas sp. (wastewater metagenome)
  ORGANISM  Petrimonas sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Dysgonomonadaceae; Petrimonas.
REFERENCE   1  (bases 1 to 3203)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3203)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:10:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,720
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 35
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3203
                     /organism="Petrimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10651_length_3203_cov_3.636595"
                     /isolate="AMR_MDS_1826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2023866"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..943
                     /locus_tag="PLI30_11895"
     CDS             <1..943
                     /locus_tag="PLI30_11895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006842850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HOI80291.1"
                     /translation="KLSVRQGYNNASFSSNGKFFINRFSDTHTPTLITVHDATGKELR
                     VLEDNKQAAALWTSAQFPNKEFISIPAADGITQLNGWIIKPRNFDASKKYPVVMIQYS
                     GPNSQQVLDRFGADWHYALANEGFLVVSVDGRGTGARGEEFRKQTYMNLGIKESDDQA
                     SAARYLATLPYIDGSRIGIWGWSYGGYNVLMSMSRGNGVFKAGVAIAPVTDWRFYDTV
                     YTERFMRTPQQNASGYTAGSAVALASQLQGKLLLIHGTTDDNVHFQNAIEYSRALIAS
                     GKHFDMFYFPDKDHFISGENSRLYLYEKVIEFYRKNL"
     gene            1207..2307
                     /locus_tag="PLI30_11900"
     CDS             1207..2307
                     /locus_tag="PLI30_11900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI80292.1"
                     /translation="MGEGSIIIKQFRNPAYGLVPLLVFTILLGWVNAGTAMCIALGLS
                     LAGVFLVRKHSRMLYDISAITFSVALLLLLFYHPLDTFGKFVIVEVIFVVALIISRLL
                     RTRVILLLSKNKKPNVRNYLSESFRVAFQVQYAFFFHLLLILVYFTVSTIKTPWISKQ
                     EIILIAQLILLTIIFLKVIRFLFLDKKLRKEEWLPVVTESGEVTGKVAKSVTKEMRNK
                     FMHPVVRVALISNGKIYLKKRDASRLLNPGALDYPFEKYMQYNHDINEAVHNTISKEI
                     GTKDIPLRFLLKYIFENSNTKRLIFLYVSIIDDEEKFNKLQLRDGKLWTEKQIEDNRN
                     SHIFSECFELEFEYLKNTVLLNYKLKPAETSC"
     gene            2416..>3203
                     /locus_tag="PLI30_11905"
     CDS             2416..>3203
                     /locus_tag="PLI30_11905"
                     /inference="COORDINATES: protein motif:HMM:NF017021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HOI80293.1"
                     /translation="MKLAKKIVRIFAIIIVCVIVLNIILFLTFNIPSVQKFAADFAIG
                     KLKPKVKTEMSIDKIRIKLFNSVEIGGLYVEDQEKDTLLFADKLSARINIWNLLDNHL
                     SIESVRLDNFTAKVYRESKDTTFNFQFIIDAFAPTDTLKKKSEKAPLKISINDIKLSN
                     GTLRYDILSRPEAPGKFNPDHFFVKNFNLNADISSLDIKHLVADIQTLSFDEQKAGVK
                     VYDLQGMVRSDGTKLWSDRLSLTLNNSHLDVSKAVYDIDSKAFNL"
BASE COUNT          941 a          625 c          682 g          955 t
ORIGIN      
        1 aaagctatcc gtgcggcagg gttataataa tgcgtcgttc agcagtaacg gcaagttttt
       61 catcaaccgg ttttcggata cccatacacc caccctcatt actgtacatg acgccaccgg
      121 taaggaactt cgcgtcctcg aagataataa gcaggcggcg gccctgtgga catctgcaca
      181 attccctaat aaagagttta tcagcatccc tgccgccgat ggcatcacac agttgaacgg
      241 ttggattatt aaaccccgta attttgatgc ctctaaaaaa tatccggtgg ttatgataca
      301 gtacagcggc cctaattcgc agcaggtgct ggaccggttt ggtgcagact ggcactacgc
      361 tctggcgaac gaaggttttt tggttgtatc ggttgacgga cggggaaccg gcgcgcgcgg
      421 cgaagagttt cgaaagcaaa cctacatgaa tttgggtata aaagagtcgg atgatcaggc
      481 atccgctgca cgctatctgg ctacacttcc ttatatagac ggtagccgta ttggcatctg
      541 gggttggagt tacggaggct acaatgtttt gatgagtatg agccggggga atggtgtttt
      601 taaagcagga gtagccattg cacctgtgac tgattggcgg ttttatgaca ctgtttatac
      661 cgaacgtttt atgcggacac cgcaacagaa tgcatcggga tacactgctg gctcggcggt
      721 tgctcttgct tcgcagttac agggaaagtt gttgctcatc catggaacga ccgatgataa
      781 tgtccatttt cagaacgcca tagagtattc gcgggcatta atcgcttccg gtaaacattt
      841 cgacatgttt tactttcccg ataaagatca ttttatctcc ggcgaaaact ctcgtttgta
      901 cctatacgaa aaagtaattg agttttacag gaagaatctg taaaacatat gtagaaactt
      961 gtgtacccgt acaattgtag tgtggtgctt gtcctgacgg agtgatggtt agtcgttccg
     1021 tcagtttttt atgacctgag ggaaagccgt aattttttgt atccctaaaa caaaaaactc
     1081 agctgggtgt tattatgaca ggtttaaaaa ttgatttaaa gcagttttgc atcaggatac
     1141 aatgaagcaa tttcttcaaa acagtttctg aatcacgatt ttttttaagc tgatcgaatt
     1201 tcgattatgg gagaaggcag tataatcata aaacaattta gaaacccagc ctacggactc
     1261 gttccgttac ttgtgtttac gattctttta ggatgggtaa atgcaggaac agctatgtgt
     1321 attgcgttgg gattatcatt ggcaggtgtt ttcctggttc ggaaacacag ccggatgttg
     1381 tatgatatct cggccatcac gttttccgtt gcacttttat tgcttctttt ttatcatccg
     1441 cttgatacgt ttggaaaatt tgtgattgtt gaagtaatct ttgttgtagc attgattata
     1501 tcccgtttac ttcgtacccg ggtaatattg ttgctgtcaa aaaacaaaaa acccaacgtc
     1561 aggaactacc tgtcggaatc ttttcgggtt gcttttcaag tacagtatgc gttctttttc
     1621 catttgttac tgatattggt atattttacc gtttcaacaa tcaagactcc ttggataagt
     1681 aaacaggaga ttatactgat cgcgcaactt atcctgctga ccataatctt tctgaaggtt
     1741 atccggtttt tgttcttaga taaaaaactg cgaaaagagg aatggctccc cgtggtaaca
     1801 gaatcgggag aagttaccgg gaaagtggcg aaatccgtta cgaaagagat gaggaataag
     1861 tttatgcatc cggtggtgag ggtggcattg ataagtaacg gcaaaattta cttgaagaag
     1921 cgcgatgctt cccgattact aaacccggga gccctcgatt atccatttga aaaatacatg
     1981 cagtataatc acgatataaa tgaagcggtc cataacacga ttagtaaaga aattggaacc
     2041 aaggatattc cattacgttt cttgcttaaa tatattttcg aaaacagcaa caccaagcgt
     2101 ttgatttttt tgtacgtttc tattatcgac gatgaggaaa agttcaacaa attgcaattg
     2161 cgggacggaa aactatggac agaaaagcag attgaagata atagaaactc tcatattttc
     2221 tccgaatgtt ttgagcttga gtttgaatac ctgaagaata cagtcttgct gaattataaa
     2281 ctaaaacctg ctgaaacgtc gtgttgaaac atcctcaact ccgccttaat ctaagttaaa
     2341 atgccttttt cccatcttta aaaattaaat ttttagtaat attgcacgtt taaccgacac
     2401 tatcggtaaa atcaaatgaa attagcaaag aaaattgtcc ggatttttgc cattattatc
     2461 gtttgtgtaa tagtattgaa tattatactt tttctgacat tcaatatccc atccgttcaa
     2521 aaatttgctg ctgattttgc tattgggaaa cttaaaccca aagtaaaaac cgaaatgtcc
     2581 attgacaaga ttcggatcaa actctttaat agtgttgaaa tcggaggttt atatgtagaa
     2641 gaccaggaga aagatacgtt gttgtttgcc gataaattat ccgcgcgcat aaatatctgg
     2701 aatttattgg acaatcattt gtctatcgag tcggtccgtt tggataattt taccgcaaaa
     2761 gtatataggg aatctaaaga cacaactttt aacttccaat tcatcattga tgcatttgcg
     2821 cctaccgaca cgttaaaaaa gaaatccgaa aaagctcccc ttaagatttc cataaacgac
     2881 attaaacttt ccaacgggac gctgcgttac gatatccttt caaggccgga agctccggga
     2941 aagttcaatc ccgatcactt tttcgtaaaa aacttcaatc tcaatgcgga catttcatcg
     3001 ctcgacatca aacatcttgt cgcggatatc caaaccttga gttttgatga acaaaaagcg
     3061 ggagtaaaag tgtatgacct acagggtatg gttcgctcag acggcacaaa actgtggagc
     3121 gacagactct cgctcacgct gaataattct cacttggatg taagcaaagc cgtttacgat
     3181 atagacagca aagcatttaa cct
//
LOCUS       DAOSDR010000363         3175 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrimonas sp. isolate AMR_MDS_1826
            NODE_10775_length_3175_cov_3.658013, whole genome shotgun sequence.
ACCESSION   DAOSDR010000363 DAOSDR010000000
VERSION     DAOSDR010000363.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863355
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrimonas sp. (wastewater metagenome)
  ORGANISM  Petrimonas sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Dysgonomonadaceae; Petrimonas.
REFERENCE   1  (bases 1 to 3175)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3175)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:10:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,720
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 35
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3175
                     /organism="Petrimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10775_length_3175_cov_3.658013"
                     /isolate="AMR_MDS_1826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2023866"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1117
                     /locus_tag="PLI30_11910"
     CDS             <1..1117
                     /locus_tag="PLI30_11910"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HOI80294.1"
                     /translation="KVFSNPHFDVSGMRLSRKRNYEIDYFAYEGEKYVILPQSTFFKD
                     FSRKMANEFKDVEYYVTDFDDDETKFLIVVQSDRVYGKYYEYDVRTGEFSLLYDLMPQ
                     LDPDDMAEMKPITFKSRDGLTLHGYITLPKAALEGEKVPLVVNPHGGPQGVRDSWGFN
                     PEAQLFASRGYATLQVNFRISGGYGKEFLRNGFKQIGRKVMDDVEDGVKHVIGQGWVD
                     PAKVAIYGGSHGGYATLMGLVKTPGLYTCGVDYVGISNIETFFASFPEYWKPLTEMAK
                     EIWYDLDNPEEAKIAREVSPIYQTDKIIRPVFVVQGANDPRVNINESDQIVTALRAKG
                     LYVPYMVKYNEGHGFYREENRMDFYGAMMGFLAKYLK"
     gene            complement(1123..1782)
                     /locus_tag="PLI30_11915"
     CDS             complement(1123..1782)
                     /locus_tag="PLI30_11915"
                     /inference="COORDINATES: protein motif:HMM:TIGR02453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2461 domain-containing protein"
                     /protein_id="HOI80295.1"
                     /translation="MNFNQIFNFLVHLEANNNRNWFNEHKSDYNQARVSFEAFVNTLI
                     VPLSQLDPSIGTITASECMFRIYRDIRFSNDKTPYKTHFSAFIANGGRKTRMAGYYVH
                     LQPDESFIAGGIYAPGPTVLESIRNSIYQHPEEIRRILDDKRFCELFPALSNEEKLKN
                     PPKGYPKDFKEVELLKNKHFVTHRALDKEFFEKADVIGRLMELFKVQYPFNDFLNRAV
                     H"
     gene            complement(1787..2365)
                     /locus_tag="PLI30_11920"
     CDS             complement(1787..2365)
                     /locus_tag="PLI30_11920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006744809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HOI80296.1"
                     /translation="MKQISFIKEIDQLKYIQRKTRLFNSNRQENDAEHSWHLAMMALI
                     LAEHSDKPVDMLKVLKMVMIHDIVEIDAGDTFIYDTSKNHTNTQEELAAAKRIFGLLP
                     KKQAEEFVEIWKEFEEGTSDEAKFARSMDRLEPLLQNISNKGGTWVEFNVDYRKVYEK
                     KKAIKEGSAALWNYAENLINDSVKEGILKKNP"
     gene            complement(2434..>3175)
                     /locus_tag="PLI30_11925"
     CDS             complement(2434..>3175)
                     /locus_tag="PLI30_11925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019539835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HOI80297.1"
                     /translation="FHYILLAIQYIGSALIYLAVRPFNETLAQAVMICVLAPTATSAP
                     VVASILGGNIASVAAFSMFSNLSVAFVAPLYLSLIGQTGSEVPFITSFWYIFRKVVPI
                     IVLPFFVALFLKKASPDLHRKVRSAQIVSFYIWAAALTVVIGNVANFVIAQDDGKYTL
                     EIVIGLISLAVCLLQFGTGRMIGSRFDRTIAGGQGLGQKNTILAIWLTQTYLNPIASL
                     GPGLYVLWQNLVNSYQIWRKNKKTEKLF"
BASE COUNT          789 a          693 c          796 g          897 t
ORIGIN      
        1 aaaagtattt tccaatcccc attttgatgt ctcggggatg aggctttcgc ggaagaggaa
       61 ttacgaaatc gattactttg catacgaggg agaaaaatac gtgattctgc cccagagcac
      121 gttctttaaa gatttttcca ggaagatggc taacgaattc aaggacgtgg aatattacgt
      181 gactgacttt gacgacgatg aaacgaagtt ccttattgtg gtacaaagcg acagggtgta
      241 tgggaagtac tacgagtacg atgtccggac cggagaattc agcctgctgt acgacctgat
      301 gccccagctc gatcccgacg acatggcgga aatgaagccg atcactttta aaagccgtga
      361 cggacttacg ttacacggat acattacctt gccgaaggca gcgctggaag gagaaaaagt
      421 ccctttggta gtgaatccgc atggcggccc gcagggagtc cgcgattcgt ggggctttaa
      481 tccggaggcc caactttttg ccagtcgtgg gtatgccacg ttgcaggtta atttccgaat
      541 atcgggcgga tacgggaagg aatttctgcg taacggcttc aagcaaatcg gccggaaggt
      601 gatggacgat gtggaggatg gcgtgaagca tgtgatcggc caaggatggg tggatcctgc
      661 taaagttgcc atttacggag gtagccacgg cggttacgcc accttgatgg ggttagtcaa
      721 gactcccggt ctctacacgt gtggcgtgga ctacgttggg atttccaata tagaaacatt
      781 ttttgcctct tttcccgagt actggaaacc acttacagag atggctaaag agatttggta
      841 cgacctggat aatcccgaag aggcaaaaat tgcccgggag gtttcgccga tttaccaaac
      901 cgacaagatc atccgtccgg tttttgtggt gcagggggcg aatgatccga gagtgaacat
      961 caacgaatcc gaccagattg ttactgcgtt gcgggccaaa ggcctgtatg taccttacat
     1021 ggtgaagtac aatgaaggac acggattcta ccgggaggag aaccggatgg atttttacgg
     1081 tgcgatgatg gggtttctgg ccaagtattt gaaatgaagc ttttagtgta ctgctctgtt
     1141 taggaagtcg ttgaacggat actgcacttt gaataattcc atcaaccgcc caatgacatc
     1201 cgccttctcg aaaaattcct tgtcgagtgc ccggtgcgtc acaaagtgtt tgtttttcaa
     1261 cagctcaacc tccttgaaat ccttcggata ccctttgggc gggtttttga gcttttcctc
     1321 gttcgagagg gcgggaaaaa gttcacaaaa ccgtttgtcg tccaaaatcc tgcgtatctc
     1381 ttccggatgt tgatagatcg agttccgtat agattccagc acggtagggc cgggagcata
     1441 aatacccccg gcgataaatg attcgtcggg ttgcaggtgg acgtaatacc cggccatgcg
     1501 ggtttttctt ccgccattgg cgataaaagc gctgaaatgg gttttgtagg gtgttttatc
     1561 gttcgagaaa cgaatatccc ggtaaatacg gaacatgcat tcactagcag ttatcgttcc
     1621 gatgctcggg tcgagctgac tgagaggcac gataagtgtg ttgacaaatg cttcgaacga
     1681 tacacgtgcc tggttgtaat cgcttttatg ttcgttgaac cagtttcggt tgttgttggc
     1741 ctccaggtgg accaggaagt tgaatatttg gttaaagttc atttttttac ggattttttt
     1801 taagaatacc ttcttttaca ctgtcgttta tcaagttctc cgcatagttc cagagggcgg
     1861 cagatccttc tttaatggct tttttctttt cgtacacttt ccggtaatcc acattgaatt
     1921 ccacccacgt cccgcccttg ttggagatgt tttgcagcaa gggttcgagc cggtccatcg
     1981 atctggcaaa ctttgcttca tcactggttc cttcttcgaa ttctttccat atttctacga
     2041 attcttcggc ttgtttttta ggtaacagac cgaaaatgcg tttggctgca gccagctctt
     2101 cctgtgtatt ggtgtgattt tttgatgtgt cataaatgaa tgtgtcgcct gcgtcaattt
     2161 ccacgatatc gtgaatcatc accattttca gtactttcag catatcaacc ggcttgtcgg
     2221 aatgttctgc caggataagc gccatcatgg ccaggtgcca gctgtgttca gcatcgtttt
     2281 cctgcctgtt gctgttgaac agtctcgttt tacgttgaat gtattttaat tggtcaatct
     2341 cttttatgaa actgatttgc ttcaataagt gatttttttc tgtttccgtt tgattttttt
     2401 gtttttcaga ctgcatggct gataatgctt tattcaaaac agtttttccg tttttttatt
     2461 ttttctccag atctggtagg aatttaccaa attttgccac agtacgtata gtccgggacc
     2521 gagtgatgct atcgggttca gataggtttg ggtaagccag atagcaagaa ttgtgttttt
     2581 ttgccccaat ccctgccctc cggcaatggt cctgtcaaat ctagacccga tcatccgtcc
     2641 tgttccaaat tgcaataggc aaacggctaa tgatatgagc ccgataacaa tctcaagggt
     2701 gtatttccca tcgtcttgcg caattacaaa attggccacg ttcccgatga ctactgtaag
     2761 ggctgccgcc cagatataaa aggaaacgat ttgcgccgag cggactttcc ggtggaggtc
     2821 gggcgatgct ttcttcaaaa acaaggccac gaaaaagggg agaacaatta tgggaactac
     2881 tttccggaaa atgtaccaaa acgaagtgat aaacggaacc tcactacccg tctggccaat
     2941 cagcgacagg taaagcgggg ctacaaaggc cacggaaaga ttactgaaca tggagaacgc
     3001 tgcaacagaa gcaatgtttc cacccaagat gcttgcaaca accggagccg aagtggctgt
     3061 aggggcaagt acgcaaatca tgaccgcctg tgccagtgtt tcattgaacg gcctgacggc
     3121 aagatagatc aatgcgcttc cgatgtattg gatagccagc aaaatataat ggaat
//
LOCUS       DAOSDR010000364         3173 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrimonas sp. isolate AMR_MDS_1826
            NODE_10787_length_3173_cov_4.061578, whole genome shotgun sequence.
ACCESSION   DAOSDR010000364 DAOSDR010000000
VERSION     DAOSDR010000364.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863355
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrimonas sp. (wastewater metagenome)
  ORGANISM  Petrimonas sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Dysgonomonadaceae; Petrimonas.
REFERENCE   1  (bases 1 to 3173)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3173)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:10:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,720
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 35
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3173
                     /organism="Petrimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10787_length_3173_cov_4.061578"
                     /isolate="AMR_MDS_1826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2023866"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(78..689)
                     /locus_tag="PLI30_11930"
     CDS             complement(78..689)
                     /locus_tag="PLI30_11930"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOI80298.1"
                     /translation="MITVHIVDDHKILVEGLQKLVDNSGFAQTTAVSYTGEDCLWKLS
                     FKQPHVILLDINLPDWSGIDLCSVIKEKYPQVKIVALTSYSEYSIVRQMLENGASGYV
                     IKNAMPEEILLGIQTVADNETFLCEEIDFLMNKRSVEQVWLTPREKILLQHIVNGYTN
                     PEIAEQMLLAVDTINNYRKKLLCKLNARNTAVLVRIALEQKLV"
     gene            complement(727..2649)
                     /locus_tag="PLI30_11935"
     CDS             complement(727..2649)
                     /locus_tag="PLI30_11935"
                     /inference="COORDINATES: protein motif:HMM:NF019350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOI80299.1"
                     /translation="MITFRKRKPTTILFLGLYLLCMNGFAQNLDSLENVLNGSDLSSE
                     EFLTVCDDLSWNYLDDNFEKSIKYAVRGIERAKSDQNLLMEARLYRNLGVAYYMNSQM
                     DTAKLFLNRSLEKAILLQDEQLEAAVYGAIGNVHNVSGNYQEALTYYLKALPMFEKHN
                     NKERIRSLLGNIATLYYSLINLSQAERYYSELEKESIEANDLYNIGRAYEGFSRIALK
                     RKDFKSSLEYSEKAAEIFHKGGYKASEAIAIQGIAMVYYLDYNNYPKAKEYALKALDL
                     TREIGYDTQIAGSLNILSNIYFNEKDYINCLKSAREAIEADTTDANVTSNLYANMVRA
                     GIFLNHHQETLRYFDKYRKLIDTRSTAEFEQAMAELEKKYQTEKKELRIRTLEKEKKL
                     NSIIFLVSTVGLFLFLLVLYLRNRTIKTNEELNKQKILQLEQENQLIAAQAVLNGETA
                     ERTRLARDLHDGLGGMLSAVKLNLFDMKQDVIIEEEDVLRFNRVMEMLDNSIGELRRV
                     AHNMMPEALSRYGLKTALSDFCKNFRNVKFHYFGAEQRVEKKLETVIYRAATELINNA
                     LKHSDAAAVNVQLVCGTDLISLNVQDNGKGFDATAETLGTGLNNIRTRVAAVNGTMSI
                     HSSPDEGTEVDVEIKI"
     gene            2852..>3173
                     /locus_tag="PLI30_11940"
     CDS             2852..>3173
                     /locus_tag="PLI30_11940"
                     /inference="COORDINATES: protein motif:HMM:NF016764.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0031267 - small GTPase binding [Evidence
                     IEA];
                     GO_process: GO:0006888 - endoplasmic reticulum to Golgi
                     vesicle-mediated transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Yip1 family protein"
                     /protein_id="HOI80300.1"
                     /translation="MEIISKVKNILLNPKTEWEVISEKNDTHLKVLTAYLIPLALIPA
                     IAGFIGYGIFGFNLMGIHASFANIGIRHAFTSFISTLGGAYLSAWIIAFLADKFESIK
                     NFDKA"
BASE COUNT          802 a          748 c          626 g          997 t
ORIGIN      
        1 cattcgtgga atacaagcct tttcgggaaa acatcgaaaa ccattaaacg gacaagggat
       61 ctacagcaca aaactgatca taccagtttt tgttccaggg ctattcgtac caggacggct
      121 gtgtttcggg cgttaagctt acataagagt ttcttccggt agttattgat ggtatccacg
      181 gcaaggagca tctgttctgc aatttcagga ttggtatatc cattcacgat atgttgcaac
      241 agtatctttt cccgtggagt cagccaaacc tgttccacgg agcgtttgtt catcagaaag
      301 tcaatttctt cacacaaaaa agtttcgttg tcagctactg tttgaatacc caacagaatt
      361 tcttcgggca tggcattttt aatgacatag cccgaagcgc cgttttcaag catttgccgc
      421 acgatagaat attccgaata actggtgagc gctactattt tcacctgcgg atatttctct
      481 tttattacgc tgcaaaggtc aatgccactc caatcgggaa gattgatatc cagcaagatg
      541 acatgcggtt gtttgaaact taatttccac aggcaatctt ctccggtata actcacggcc
      601 gtggtctggg caaatccgga attatccacc aacttttgca acccctctac caggatctta
      661 tggtcgtcta ctatatgaac ggttatcatt ttgctttttc ttgttttctg ctggcctgtc
      721 cccccgctat atctttatct ccacatccac ctcggtcccc tcgtccggag aagaatggat
      781 gctcatggtt ccgttcactg ccgcaacgcg tgtccggata ttgttcaggc ctgtccccaa
      841 agtttcggcg gtcgcatcaa accccttacc attgtcctgg acgttgaggg agatcagatc
      901 ggtgccacac accagctgca catttacagc agctgcgtcg gaatgcttta aagcattgtt
      961 tatcaactcg gtcgcggctc gataaataac cgtttcgagt ttcttttcaa cccgctgttc
     1021 tgcaccgaag taatgaaatt ttacgtttct gaagttttta cagaagtcgc tcaaggctgt
     1081 cttaagccca tagcgcgaga gtgcttcggg catcatgttg tgtgccacac gccggagctc
     1141 cccgatggaa ttgtccagca tttccatcac tctgttgaaa cgtaaaacat cttcctcttc
     1201 gataattaca tcctgcttca tatcgaataa atttaatttt actgccgaga gcatccctcc
     1261 caatccatcg tgaaggtcgc gtgcaagccg ggtacgctct gcagtttcgc cattcagtac
     1321 ggcttgcgcg gcgatgagct ggttctcctg ttcgagttga agaatttttt gcttgttaag
     1381 ttcctcgttc gtttttatcg ttctgttgcg taaatacaat accaacaaaa agagaaacaa
     1441 cccaaccgta ctcaccagga agataatgga attaagtttt ttttctttct ccagcgtgcg
     1501 tatcctgagc tcttttttct cggtctggta cttcttttcc agttccgcca ttgcctgctc
     1561 aaactctgcc gttgagcggg tatcgatcag cttccggtac ttgtcgaagt atcgcaacgt
     1621 ttcctggtga tggtttaaaa atatccctgc ccttaccatg ttcgcataca ggtttgaggt
     1681 tacgttggca tccgttgtgt ctgcttctat tgcttctctg gccgacttca agcaattgat
     1741 gtaatctttc tcgttaaaat aaatgttcga caggatattc aacgaaccgg caatctgtgt
     1801 gtcataccct atctcccggg ttaaatccaa tgctttcagt gcgtattctt tagctttcgg
     1861 gtaattgtta taatccaggt aataaaccat ggctattccc tgaatcgcaa tagcttccga
     1921 tgccttatac cctcccttgt ggaatatttc cgccgccttt tcagaatatt ccaacgatga
     1981 tttaaaatct tttcgtttca gggctatcct gctgaatccc tcgtatgcac gccctatatt
     2041 atacagatca ttggcctcta tactctcttt ttccagttcc gaatagtatc tttcggcctg
     2101 gcttaagttt atcaaactgt aatataaagt agcaatgttt ccgagcagcg accgtatgcg
     2161 ttccttgttg ttgtgtttct caaacatagg gagagctttc aggtaatagg taagggcttc
     2221 ctgatagttc cccgatacgt tgtgcacatt tccgatagcc ccgtaaacag cagcttccag
     2281 ttgttcatct tgcaggagga tagctttttc aagcgatctg tttaaaaaca gtttagccgt
     2341 atccatctgg ctgttcatgt aataggctac tcctaaattc cggtaaagcc gtgcctccat
     2401 aagcaggttc tggtccgact ttgctctttc aataccccgc acagcgtatt ttattgattt
     2461 ttcaaaatta tcgtcgagat agttccagct taagtcgtcg cataccgtca ggaactcttc
     2521 cgatgacagg tcactgccgt tcaacacatt ttccaaacta tctaaattct gagcaaaccc
     2581 gttcatgcaa agcaagtaca gcccaagaaa caggatagtc gttggttttc ttttcctgaa
     2641 agtgatcatt gtgtttagtt attagcattt tagtacgttg acagcagcaa atgtagattg
     2701 tattgccgac acaaaattaa atttattcac agtaattaaa aactcccttt tgggtctatt
     2761 ttttactttt cccagtccgt aaatttgtat cctaagaatc ggataacgtg agaaaaatac
     2821 ttcaataaac aaaagtaaaa atcagtgttt tatggaaatt attagcaaag taaaaaacat
     2881 tttgttgaat cccaaaacag aatgggaagt aatatcggaa aaaaacgata cacacttaaa
     2941 agtattgact gcttacctga ttcccttggc gctcatccct gctattgcag gattcattgg
     3001 atacggaatt ttcggattca acctgatggg aattcatgcg agtttcgcaa atatcggtat
     3061 ccgtcacgca tttacttcat ttatctctac actaggagga gcttacctgt cggcatggat
     3121 cattgccttt ctcgccgata agtttgagtc catcaaaaat ttcgataagg cat
//
LOCUS       DAOSDR010000365         3172 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrimonas sp. isolate AMR_MDS_1826
            NODE_10790_length_3172_cov_3.772538, whole genome shotgun sequence.
ACCESSION   DAOSDR010000365 DAOSDR010000000
VERSION     DAOSDR010000365.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863355
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrimonas sp. (wastewater metagenome)
  ORGANISM  Petrimonas sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Dysgonomonadaceae; Petrimonas.
REFERENCE   1  (bases 1 to 3172)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3172)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:10:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,720
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 35
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3172
                     /organism="Petrimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10790_length_3172_cov_3.772538"
                     /isolate="AMR_MDS_1826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2023866"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..374)
                     /locus_tag="PLI30_11945"
     CDS             complement(<1..374)
                     /locus_tag="PLI30_11945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019537760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:calcium antiporter"
                     /protein_id="HOI80301.1"
                     /translation="MEVVWLLVSLVILYFGAEWLVSGASSFAARLGVSPLIIGLTIVS
                     MGTSAPELVVSVKAAMNGQSALSIGNVLGSNLFNVGIILGISALIYPLAIKRQLLKFD
                     APVMVLTAVLFFVLFLDGRISSV"
     gene            complement(486..1004)
                     /locus_tag="PLI30_11950"
     CDS             complement(486..1004)
                     /locus_tag="PLI30_11950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019537761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HOI80302.1"
                     /translation="MKSKSSFENRLLGLQDNMFNFALTLTADREDAKDLLQETTLRVL
                     DNREKYYENVNFKGWVFTIMHNIFVNNYRKIVRSQTIIDKTENLYHLNLPQDSGFNTP
                     DGAYTIKEINKAIASFTDEYKVPFSMHVSGYKYEEIAQHLGLPIGTVKSRIFFARKRL
                     QEMLKDFRFYTE"
     gene            1359..>3172
                     /gene="htpG"
                     /locus_tag="PLI30_11955"
     CDS             1359..>3172
                     /gene="htpG"
                     /locus_tag="PLI30_11955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006842744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HOI80303.1"
                     /translation="MIKEGQIGVTTGNIFPIIKKFLYSDHEIFLREVISNAVDATQKL
                     KTLSDVGEFKGDPGDLSVRVVIDKDTKTLTISDRGIGMTADEVDKYINQIALSSANDF
                     LDQYKDNANAIIGHFGLGFYSSFMVSKKVEIVTKSYKEEPAVKWSCEGDPAYKMEEAD
                     KTGRGTDIILYIDEENEAFLEKSKIESLLDKYCKFLPVPIVFGKKQEWKDGKYVDTEE
                     DNVINDVNPLWKQKPVGLKDEDYRDFYRKLYPMSMDEPLFWIHLNVDYPFNLTGILYF
                     PKVKSNIELQRNKIQLYSNQVFVTDSVEGIVPEFLTLLHGVLDSPDIPLNVSRSYLQS
                     DNNVKKISNHITKKVADKLEEIFKKERTQLEEKWDSLKIFVEYGMLTEERFYDRASKF
                     ALLKNTDNKYFTLDEYKALTESSQTDKNSTLVYLYANNKEDQYTFIDAAKGKGYDVLM
                     MDGQLDVHWLGLLEQKLEKTRFVRVDSDTVEHIIEKDEKTSVELSDKEKENLTEAIQS
                     LLPNIDKTEFNVDFKSLGETTRPVQITQNEFSRRMKEMSAMQPGMAFYGEMPDMFQVI
                     VNMDHPIAKQLLGEVKDKSKEETTAFASENARLKQVIDL"
BASE COUNT          955 a          710 c          739 g          768 t
ORIGIN      
        1 acagacgaaa tcctaccgtc gagaaacaac acgaagaaca gtaccgctgt gagtaccata
       61 accggagcgt cgaacttcaa cagctgcctt ttaatggcca gcggatagat aagcgccgaa
      121 atgcccaaaa tgattccgac attaaacagg ttggaaccca gtacatttcc aatcgacaag
      181 gcgctttgcc cgttcatcgc ggcttttacg ctaaccacca gctcgggagc gctcgtcccc
      241 atggaaacaa tggtcagccc gataatcaac gggctgacac ccaaccgggc agcaaaggac
      301 gatgcacctg acacgagcca ttcagctccg aaataaagta tgacgagtga aaccaataac
      361 caaacaactt ccattaacaa ctagttttat gagtaaggag ggcacaaagc cgtacaggta
      421 cctataacaa caaaaaaccc ctttggttca aatggccaaa ggggtttttt tataagggtt
      481 tcgttttatt ccgtatagaa ccggaaatcc ttcagcatct cctgcaagcg tttgcgggcg
      541 aaaaagatcc ggcttttaac cgtgccgatg ggaagcccca ggtgttgcgc aatttcttcg
      601 tacttatatc cggaaacgtg catggagaag ggaactttgt attcatcggt gaagctggca
      661 atggccttgt tgatctcctt gatggtgtag gctccgtcgg gagtattgaa cccggaatcc
      721 tgtggcaggt tcaggtgata caaattctct gttttgtcga taattgtctg gcttctgaca
      781 attttgcggt agttattcac aaatatattg tgcatgatgg tgaaaaccca tcctttaaaa
      841 ttgacgttct cgtaatactt ttcccggttg tccaaaaccc ggagagttgt ttcctgcaaa
      901 agatcttttg catcttcccg atctgctgtt aaagttaatg cgaagttaaa catattatcc
      961 tgcaagccca gcaatctgtt ttcgaaagaa ctttttgact tcatagcttt aatttttgat
     1021 tgtgttgtct tttgcaaata taataacata atttactccg tgaacatttt tttatttcaa
     1081 aaacaattaa ttagtgttaa caaatcagcc caaatcattt tgcgatttgt tattcagcac
     1141 gttacacata tttcatgtgg ggattttctc cacacatgat cgataaataa tatttatatc
     1201 gtgatttgga taatctaaaa acgcaatatt tccagcagaa cggtgatttt agatcacgtc
     1261 taaataagcc ggatgaaggc aggttagccg gcatttttat ggcatactat ttgcaaataa
     1321 tttgaaagat taaaattaac accatatagt attatagtat gataaaagaa ggacaaattg
     1381 gggtaacaac cggaaatatt ttccccatca tcaagaaatt tctttacagt gaccacgaaa
     1441 tttttcttcg tgaagttatc tccaacgcag tggatgctac gcaaaagctg aaaacattgt
     1501 cggatgtagg agaattcaaa ggtgacccgg gcgatttatc cgtcagggta gttatcgata
     1561 aggataccaa aacgctgacc atctccgatc gcgggatcgg aatgacggct gacgaagtgg
     1621 acaagtacat caaccagata gcgctttcct cggccaacga tttcctcgat cagtacaagg
     1681 acaatgccaa tgccattatc gggcatttcg ggctgggttt ttattcctcg ttcatggtgt
     1741 ctaagaaggt ggagatcgtc accaaatcct acaaggagga acctgcggtg aaatggtcgt
     1801 gcgaaggcga tccggcctac aagatggagg aggcggacaa aaccggccgc ggcacggaca
     1861 ttatactcta cattgacgaa gagaacgagg cgttcctgga aaagagcaag attgaatcgt
     1921 tgctggacaa gtactgtaag tttttaccgg ttcctatcgt ttttgggaag aaacaggagt
     1981 ggaaagacgg aaagtacgtg gataccgaag aagataacgt tatcaacgat gtgaatccgc
     2041 tctggaaaca aaaacctgtt ggtttaaaag acgaggatta ccgcgatttt taccgcaagc
     2101 tctatccgat gagcatggac gaaccgctct tctggatcca cctgaacgtg gattatccgt
     2161 ttaacctcac gggaatactt tatttcccga aggtcaaaag caacatagag ttgcaacgga
     2221 acaagataca gctatacagc aaccaggttt tcgtgaccga ttcggtggaa gggattgtcc
     2281 ccgagtttct gacgttgctt cacggtgtcc tcgactcgcc cgatatcccg ctcaacgtgt
     2341 cgcgctcata cctgcaaagc gataacaacg tgaagaagat ctcgaaccac atcaccaaga
     2401 aggtggccga caagctggag gagattttca aaaaggaacg tacgcagctc gaagagaaat
     2461 gggatagcct gaaaattttc gttgaatacg gcatgcttac cgaagagcgt ttttacgaca
     2521 gggcttccaa gtttgcactg ctcaagaaca ccgacaacaa gtacttcacg ctggacgagt
     2581 acaaggccct taccgaaagc agccagaccg acaagaacag caccctggtg tacctgtatg
     2641 ccaacaacaa ggaagaccag tacaccttta tcgatgccgc caaagggaaa gggtacgacg
     2701 tgctgatgat ggacgggcag ctggatgtgc attggctggg acttctcgaa cagaagctgg
     2761 agaaaacccg tttcgtacgc gttgacagcg acacggtgga gcacatcatc gagaaagacg
     2821 aaaaaacctc cgtggaattg tccgacaagg agaaggagaa cctgacggaa gccatccaat
     2881 cactgcttcc caacatcgac aagacagagt tcaacgtcga cttcaaatcg ctgggcgaaa
     2941 ccacacggcc ggttcagatc acccaaaacg aattttcacg aaggatgaag gagatgtcgg
     3001 ccatgcagcc ggggatggct ttctacggag agatgcccga catgttccag gtaattgtaa
     3061 acatggacca tccgattgcc aaacaacttc tcggtgaggt caaagataaa tcgaaggaag
     3121 agacaacggc atttgcgtcg gaaaacgcca gactgaaaca ggttatcgac ct
//
LOCUS       DAOSDR010000366         3167 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrimonas sp. isolate AMR_MDS_1826
            NODE_10810_length_3167_cov_3.999357, whole genome shotgun sequence.
ACCESSION   DAOSDR010000366 DAOSDR010000000
VERSION     DAOSDR010000366.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863355
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrimonas sp. (wastewater metagenome)
  ORGANISM  Petrimonas sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Dysgonomonadaceae; Petrimonas.
REFERENCE   1  (bases 1 to 3167)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3167)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:10:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,720
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 35
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3167
                     /organism="Petrimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10810_length_3167_cov_3.999357"
                     /isolate="AMR_MDS_1826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2023866"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..269)
                     /gene="raiA"
                     /locus_tag="PLI30_11960"
     CDS             complement(<1..269)
                     /gene="raiA"
                     /locus_tag="PLI30_11960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005782154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_component: GO:0022626 - cytosolic ribosome [Evidence
                     IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HOI80304.1"
                     /translation="MELRIQSINFEATEQLKAFVEKKIKKLEKFSDNIIQTEVLLKVI
                     KPETAKNKEASVKINLKNGEAFASKVADTFEEAIDLCAEALEKQIL"
     gene            complement(283..1173)
                     /locus_tag="PLI30_11965"
     CDS             complement(283..1173)
                     /locus_tag="PLI30_11965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019538908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HOI80305.1"
                     /translation="MWKEKWIQHLRNEKNYSSHTEISYLTDLTQFQQFIEEECGEFDP
                     KQIDSDLVRLWIAHLVEEGIKPRSANRKLSAVKTFFNYLNKIGATNGNPAEKIRGPKT
                     PKKLPAFVHHEELTRVIDDECAYPDNFEGQRDRFIIELFYVTGMRKSELIGIKNSDVD
                     NYAKTLRVTGKRNKQRIIPLSDDTVEKLKKYIQSRDREIENKSPHLFVKKDGNPMYPK
                     MIYNIVRKHLDSIPTLSKRSPHVLRHSFATEMLNNGAEINAVKELLGHSSLASTEVYT
                     HVTFEELKKVYHNAHPRAKN"
     gene            complement(1232..1423)
                     /gene="rpsU"
                     /locus_tag="PLI30_11970"
     CDS             complement(1232..1423)
                     /gene="rpsU"
                     /locus_tag="PLI30_11970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007134585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HOI80306.1"
                     /translation="MIIVQLKEGENIERALKKFKRKYERTGVVKELRGRQAFAKPSVV
                     KRKKKQHAVYVQKMQENED"
     gene            complement(1665..3083)
                     /locus_tag="PLI30_11975"
     CDS             complement(1665..3083)
                     /locus_tag="PLI30_11975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008640309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HOI80307.1"
                     /translation="MKKITKRVFVILFLSSALFSLNAEIRMPVIFGDNMVLQQQSEAA
                     IWGWAKANTSVRVTTSWNNKSYSAKSDGQGYWKLKVKTPAAGYTPYTITISDGKAITL
                     NNILIGEVWICSGQSNMEMPMKGFGNQPIEGGPEAIATSRNPGIRCFTMKKASKSAPQ
                     ADGEGTWEIAGPETTPDFTATGYYFGRLLNQALDVPVGLIHTSWGGSRIEAWMTPSSI
                     KSVIPDVQIPATDADIKSQNGTPTVLYNGMLHPIVGYGIRGAIWYQGESNRDEPARYV
                     ELFDKMVREWRTIWEVGEFPFYYCQIAPFNYGGGLNSGFIREAQAKGMKTTPNTGMAV
                     LMDSDSPTCIHPPKKKIAGERMALWALAKTYGMNKMHYRSPEVQSLTVEGRVAILTMD
                     ISSSPGLTSHGKEILQFQIAGDNKWFHPAKAAIAGNRIFVFSPNVEKPVAVRYCFNDT
                     EATEIFTVEGNLPLSSFRTDNW"
BASE COUNT          715 a          684 c          720 g         1048 t
ORIGIN      
        1 agaatctgtt tttccaatgc ttcggcgcat aaatcaatgg cctcttcaaa cgtgtcagcg
       61 actttgctgg caaaggcttc tccgtttttc aagttgattt ttacagatgc ttccttgttc
      121 tttgcagtct ctggtttgat tacttttagg agcacttcgg tttgaatgat gttgtcgctg
      181 aatttttcga gttttttgat ctttttttca acaaaggctt tcagttgttc tgtcgcttcg
      241 aaattgatag actgaatacg taattccata atctttcctc ctctaatttt ttgctctggg
      301 atgagcgtta tgatagactt tttttagttc ttcgaaggtt acgtgtgtgt aaacttcggt
      361 agatgccagg ctcgagtggc ccaacagctc cttgacggca tttatttcgg caccgttgtt
      421 aagcatctcg gtagcgaaag agtgccgcaa cacgtgcggg cttcttttcg ataaggtagg
      481 aatgctgtcg agatgttttc gaacgatgtt gtaaatcatt ttgggataca tggggtttcc
      541 gtctttttta acaaacaggt gtggcgattt gttttctatt tcccgatccc ttgattgtat
      601 atattttttc agtttttcaa cggtgtcgtc cgaaagaggg atgatgcgct gcttattcct
      661 ttttccggtg acgcgaagtg ttttggcata gttgtctacg tctgagtttt ttataccgat
      721 gagttctgat tttcgcatac cggtcacata gaacagctca ataataaacc ggtcgcgttg
      781 cccttcaaaa ttatcgggat atgcacattc atcgtcgata acccgggtca actcttcatg
      841 atgaacaaaa gcaggtagtt tttttggcgt ttttggacct cttatttttt cggcgggatt
      901 tccgttggtg gccccgatct tattcaggta gttaaagaag gtttttactg cactcagttt
      961 tcggtttgcc gaccggggtt taatgccttc ttctaccagg tgggctatcc agagccggac
     1021 aaggtcgctg tcgatttgtt tcggatcgaa ctctccacac tcctcctcaa taaattgctg
     1081 gaattgcgtg aggtctgtca aataagaaat ctccgtatga gaggaataat ttttctcatt
     1141 acggagatgc tgtatccatt tttccttcca catatcgtaa ctcagccggt ttgatatgcg
     1201 aagttaggaa attttttttt aattgtgaaa attaatcttc gttttcttgc attttttgta
     1261 cgtaaacggc gtgttgtttt ttcttgcgtt ttacaactga aggtttggca aaagcctggc
     1321 gtccgcgaag ttctttcaca acgcctgttc tttcgtattt acgtttaaat ttcttcaagg
     1381 ccctttcgat gttttcgcct tcttttaatt gtacgatgat catgttttta ttttttttga
     1441 gattattgtt tgtttgaaaa aatatacaca aatatatgca tatgtgtaat tgtcacgggc
     1501 taagagcacg tttgccccga cgagtgaaat aaatggtctt ttattctata ctgttttatt
     1561 tccgagcgtt acaataacct cccttctgtt ttgcgggtgc aaagatataa aataaaaatt
     1621 taactgtcaa tgctatgggc atctatttat atttttttta ccagttacca gttgtcggta
     1681 cggaaagaag aaagggggag gttgccttcg accgtaaaga tttctgttgc ctcggtgtcg
     1741 ttaaagcaat aacggacggc taccggtttt tctacgttag gagagaatac aaaaatccgg
     1801 tttccggcaa tagccgcctt ggccggatgg aaccatttgt tgtctccggc aatctgaaat
     1861 tgtaggatct ctttcccatg tgaagtgagt cccgggctgg aagaaatgtc catcgtgaga
     1921 atggctacac gcccttccac ggttagggac tgaacttcag gactgcggta atgcatcttg
     1981 ttcatcccgt atgttttcgc caacgcccaa agcgccatgc gttcgccggc tattttcttc
     2041 ttggggggat gaatacaggt ggggctgtcc gaatccatca gaacggccat tcccgtgttg
     2101 ggtgtggttt tcatcccttt ggcctgtgct tcacgaataa aacccgaatt aagtccacct
     2161 ccgtaattga agggtgcaat ctggcagtaa taaaacggga actcgcccac ctcccagatt
     2221 gtacgccatt cgcgtaccat tttgtcaaac agttccacat acctggcggg ttcatcccgg
     2281 tttgattcac cctgatacca gatagcaccg cggataccgt atcccactat ggggtgcagc
     2341 attccgttat acaatacggt gggtgtcccg ttttgggact ttatgtcggc atcggttgcg
     2401 ggtatctgaa catcgggaat cacgctttta atggaagagg gggtcatcca ggcttcgatg
     2461 cgcgaaccac cccagctggt gtggatcagt ccgacaggaa catccagcgc ctgattcagc
     2521 aagcggccga agtaatagcc tgtcgccgtg aaatcaggtg ttgtctccgg tcccgctatt
     2581 tcccaagtac cttcaccgtc ggcctgaggg gctgacttgg aagccttttt catggtgaag
     2641 catcggatgc cgggattccg ggaggtggca atcgcttccg gccctccctc gatcggttgg
     2701 tttccaaatc ctttcattgg catctccatg ttggattgtc cagaacaaat ccatacttcc
     2761 ccgatcagga tattattcaa cgtaattgct tttccgtcgc ttatggtgat ggtatagggt
     2821 gtatagcctg ctgctggtgt ctttaccttc agcttccaat acccttgtcc gtcacttttt
     2881 gccgaatagc ttttattgtt ccaggaggtg gttacccgta cggaggtgtt tgcttttgcc
     2941 caaccccaga tcgcagcctc cgattgttgc tgcaatacca tattatcacc aaaaatgaca
     3001 ggcatcctga tctccgcgtt caatgagaac agggcggaag acaggaataa aataacaaat
     3061 actcgttttg taattttttt catgacacgt gaataatgtt tctttattaa attgttggca
     3121 aaaatagtca ttttttttaa aattaccgtt tgaatggata tctggtg
//
LOCUS       DAOSDR010000367         3145 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrimonas sp. isolate AMR_MDS_1826
            NODE_10911_length_3145_cov_3.354045, whole genome shotgun sequence.
ACCESSION   DAOSDR010000367 DAOSDR010000000
VERSION     DAOSDR010000367.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863355
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrimonas sp. (wastewater metagenome)
  ORGANISM  Petrimonas sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Dysgonomonadaceae; Petrimonas.
REFERENCE   1  (bases 1 to 3145)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3145)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:10:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,720
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 35
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3145
                     /organism="Petrimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10911_length_3145_cov_3.354045"
                     /isolate="AMR_MDS_1826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2023866"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..384
                     /locus_tag="PLI30_11980"
     CDS             <1..384
                     /locus_tag="PLI30_11980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019541350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OPT/YSL family transporter"
                     /protein_id="HOI80308.1"
                     /translation="GAVISILINWLNISPLAFALGMFIPLPLNTPLVVGGLLNHWISK
                     RSKDPVLNNARHQRAILIASGFIAGAALFGVIGALVIFVTGNGNALNLRIWEDPHGTG
                     AQVTALIAFTALITYFVWEAMRAKK"
     gene            409..2616
                     /locus_tag="PLI30_11985"
     CDS             409..2616
                     /locus_tag="PLI30_11985"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019541351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH92 family glycosyl hydrolase"
                     /protein_id="HOI80309.1"
                     /translation="MKKILFCVTAAVLAVACTQQKQGIKTDESDYTKYVNPFIGTAFT
                     GHTFPGATYPLGMIQAGPQTGNFSWAYCSGYFYEDSLIQGFTQNRLNGTGCVDLGDLL
                     VQPLSGEKRDSFDSRFDKATEKASPGYYAVQLLDNEVNVEITASPHVAFHKYAFPQGK
                     AANILADFQSGLVWQDARFHTHVLDNEVTFESDRVISGFTRRTEWVERIYYFVIEFDK
                     PIVTKEELPKRSPLEKSSRYVLTFDMHNADTLNMKIGMSSASVEGAKANLAAEVPGWD
                     FESVLQDAKKGWNSYLAKVKIEGSEDDKVNFYTSMYHLYIQPNNIADVDGKYTGPSRK
                     VSQSPNGKYYSTWSQWDTFRAAFPMYTILTPELIPDFVNSMLDYSEQQGHLPIWSLWG
                     QETYTMIGNHSVPMIVDAYLKGFTGFDAERAYNEIKKSITESKHYKSDWDIYDKFGYY
                     PYDLIKVESVSRTLECGFDDYCMAVFAEKLGKTEDAAFFRKRADYYKNHYDKATNSMR
                     PKDSKGKWLTPFDPYALAHADSNVGGHYTEGNALQYTWHVMQDIPGLIEWMGGKEKAG
                     EVLDYLFNTEQESTGTLSDVTGLIGQYAHGNEPSHHVAYIYPYLDRPGETQRLVRKIC
                     TDFYKNKPDGLIGNDDCGQMSAWYMFSAMGFYPVNPVSGEFVLGAPQVTSASLDIGNG
                     KWFTMEAKNLSKENLYVEKVELNGKPYGKRTITYKDIMDGSSLVFYMTSEVKK"
     gene            2750..>3145
                     /locus_tag="PLI30_11990"
     CDS             2750..>3145
                     /locus_tag="PLI30_11990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI80310.1"
                     /translation="MRNVDLLFCFLCLNFLLSGYCIENIHSQPIKNDFFVDITPNQFK
                     GSDIERIQQAIRLAQKTSRKVVIPQSNANGTLAWKIDRAILLPSDITIILDNCTIQLS
                     DECRDNMYRSENVGTGIQHPVWLNNIHLIG"
BASE COUNT          924 a          694 c          739 g          788 t
ORIGIN      
        1 ggcgcggtca tctctattct catcaactgg ctgaacatat ccccgttggc gtttgcgctc
       61 gggatgttta tcccgctacc gttaaacaca ccgcttgtag tggggggatt actcaaccac
      121 tggatcagca aacgttcaaa agatccggtg ctcaacaatg cccgccatca acgtgcgata
      181 cttattgcat cgggttttat agcgggcgct gcactgttcg gcgttatcgg cgcactggtc
      241 atttttgtta caggcaatgg gaatgcactc aacttgcgta tttgggaaga cccgcacggc
      301 acaggagctc aggttactgc tttgatcgct ttcaccgcgc tgatcactta ttttgtctgg
      361 gaagccatga gggcgaagaa atgaactgaa ataaagaaca acaagacaat gaaaaaaatt
      421 cttttttgtg tgacggcagc cgtgttggct gttgcctgca cacaacaaaa acaaggtata
      481 aagaccgatg aatccgatta caccaagtac gtgaatccat ttatcgggac agcttttacc
      541 ggccatacat ttcccggggc aacctatcct ctgggaatga ttcaggccgg accgcaaacc
      601 ggcaattttt cgtgggcata ctgctcggga tacttctacg aggattcgct gattcaggga
      661 tttacacaaa accgattaaa cggaaccgga tgcgtagatc tgggagattt gctggtacaa
      721 ccgctttcgg gagagaagcg ggatagtttt gacagccgtt tcgacaaagc tacggaaaaa
      781 gcttctcccg gatattatgc cgtacaactt ttggataacg aagtgaacgt agagattacg
      841 gcgtcgccac acgttgcttt tcacaaatat gcttttcctc aagggaaagc ggccaatatc
      901 ctcgccgatt ttcaaagcgg actggtgtgg caggacgctc gttttcacac acacgtgctt
      961 gataatgaag taaccttcga aagtgaccgg gtaatttcgg gttttacgag acggacggaa
     1021 tgggtggaac ggatttatta ctttgtaatc gaatttgaca aaccaattgt aaccaaagaa
     1081 gaacttccga aacgtagtcc gcttgaaaaa tcgtcacgct acgttcttac gttcgacatg
     1141 cacaacgccg atacgctaaa catgaaaatt gggatgtcgt ctgcaagcgt ggaaggagca
     1201 aaagccaacc tggcagccga agttccaggc tgggatttcg aatcggtgct gcaggatgca
     1261 aaaaagggat ggaacagcta cctggcaaaa gtaaaaatag agggatcgga agacgataaa
     1321 gtgaacttct atacatcgat gtatcacctg tatattcaac ccaacaacat tgccgatgtg
     1381 gatgggaaat atacgggtcc gagccgtaaa gtgtcgcaat cacccaacgg aaaatactat
     1441 tccacgtggt cgcagtggga tactttccgt gcagcctttc ccatgtatac catcctcacc
     1501 cctgaactta tacccgattt tgtgaactca atgctcgact acagcgagca gcaggggcat
     1561 ctgcctatct ggtcgttgtg gggacaggaa acttacacca tgatcgggaa ccattctgtt
     1621 ccgatgatcg tagatgctta cctgaaaggg tttacgggat tcgatgctga acgggcctat
     1681 aacgaaataa agaaatccat taccgaaagt aaacactata agtctgattg ggatatatac
     1741 gacaaattcg gatattatcc gtatgatctg atcaaggtag agtcggtttc ccgtacactc
     1801 gaatgtggat tcgacgacta ttgcatggct gtttttgcag aaaaactggg caaaacggag
     1861 gatgctgctt ttttcagaaa acgggccgat tattacaaaa atcactacga caaagcgacc
     1921 aactccatgc gtccgaaaga ctcaaaaggg aaatggctta cgcctttcga tccgtatgca
     1981 ctggcgcacg cagattcaaa tgtcggcgga cattacaccg aaggaaacgc cctccaatac
     2041 acgtggcatg tgatgcaaga tattccggga ttaattgaat ggatgggcgg aaaggaaaag
     2101 gcaggtgaag tgctggatta tctgttcaac accgagcagg aatcgaccgg aacgctatca
     2161 gacgtaacgg gacttatcgg acaatatgca cacggaaacg agccgagcca ccacgtagcc
     2221 tacatttacc cctacctcga ccgtcccggg gaaacgcaac gcctcgtacg gaagatctgc
     2281 accgattttt acaagaacaa acccgatgga cttatcggga acgatgattg cgggcaaatg
     2341 tcggcctggt atatgttctc ggcaatgggt ttttatccgg taaaccccgt aagcggcgag
     2401 tttgtgctgg gcgccccaca agttacttcg gcaagccttg atataggcaa cggcaaatgg
     2461 tttacgatgg aggccaaaaa cctgtcgaaa gaaaacctct acgtcgagaa agtggaactt
     2521 aacggaaagc cgtacggcaa gagaaccatt acgtataagg acataatgga cggaagttca
     2581 ttggtgtttt atatgacaag tgaagttaaa aagtaataag ttttatctgt tgtgtattta
     2641 gtaaaaatta attggggtgt tgtttctgtt tctgtggatt ggatttccgg aatagattaa
     2701 aaacaaccat ccacctttga tttttaattt ttaaataata aagacgaaaa tgagaaacgt
     2761 tgatttgttg ttttgttttt tgtgtttgaa ttttctttta tccggctatt gcatagaaaa
     2821 catccactca caaccaatta aaaatgattt ttttgtagac atcactccca atcaattcaa
     2881 aggttctgac attgaacgaa tccaacaagc tattcgtctt gcccaaaaaa cttcccgaaa
     2941 ggtggtcatc ccgcaaagca atgccaacgg tacacttgct tggaaaatag accgggctat
     3001 tttactgcct tcagacataa ccatcatttt agacaattgc accatccaac tttccgatga
     3061 atgccgcgac aatatgtaca gaagcgaaaa tgtaggaaca ggaattcaac atcctgtatg
     3121 gttgaataac atacacctta tcgga
//
LOCUS       DAOSDR010000368         3124 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrimonas sp. isolate AMR_MDS_1826
            NODE_11022_length_3124_cov_3.314761, whole genome shotgun sequence.
ACCESSION   DAOSDR010000368 DAOSDR010000000
VERSION     DAOSDR010000368.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863355
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrimonas sp. (wastewater metagenome)
  ORGANISM  Petrimonas sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Dysgonomonadaceae; Petrimonas.
REFERENCE   1  (bases 1 to 3124)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3124)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:10:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,720
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 35
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3124
                     /organism="Petrimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11022_length_3124_cov_3.314761"
                     /isolate="AMR_MDS_1826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2023866"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..260
                     /locus_tag="PLI30_11995"
     CDS             <1..260
                     /locus_tag="PLI30_11995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007661265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF933 domain-containing protein"
                     /protein_id="HOI80311.1"
                     /translation="LQTFLTAGPQEVRAWTFQKGWKAPQCAGVIHTDFEKGFIRAEVI
                     KYADYIQYGSEATVKEAGKMNVEGKEYIVQDGDIMHFRFNV"
     gene            394..1410
                     /locus_tag="PLI30_12000"
     CDS             394..1410
                     /locus_tag="PLI30_12000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019538183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HOI80312.1"
                     /translation="MKRVSLKDIARELGVSTATVSLVLNGKNMSGRVSSEMSQKILDK
                     ASELNYIPNTLAKGLKVGKSRTIGLIIADISNLFFGALALHIQIYAEQRGYSVIIRNT
                     NEQLNEMENLMKFLYARQVEGLIITPTENSQYLLKRLISNKIPFVLVDRSFPELPVNS
                     VLINNYEISYRSTGQLIKKGCKRIGLITYKQDHYHINERKRGSVDAMKHAGIYDPGMV
                     EEVRYDFLKNDIKRAICNLISKKEKTDGLFFTTNSISINGVKELIRNNINIQKDIQIM
                     CFDENDAFYILPYAVPFIKQPIKEMAKNAVELLIDQIEMKSEGTKNVVVGAELVLNEN
                     MMYL"
     gene            1540..>2067
                     /gene="fucI"
                     /locus_tag="PLI30_12005"
     CDS             1540..>2067
                     /gene="fucI"
                     /locus_tag="PLI30_12005"
                     /EC_number="5.3.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019538182.1"
                     /note="catalyzes the conversion of the aldose L-fucose
                     into the corresponding ketose L-fuculose; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose isomerase"
                     /protein_id="HOI80313.1"
                     /translation="MKQNYPKIGIRPIIDGRRGGIRESLEETTMNLAKSAAELYSGTL
                     KYPDGSPVKCVIADTTIGGVKEAALCAEKFKKEGVGLTLSVTPCWCYGSETIDMDPLM
                     PKAVWGFNGTERPGAVYLSAALAVHNQKGLPAFGIYGKNVQDVGDGAIPDDVKEKLLR
                     FARAGLAVAIMRGKSY"
     assembly_gap    2068..2077
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2078..>3124
                     /gene="fucI"
                     /locus_tag="PLI30_12010"
     CDS             <2078..>3124
                     /gene="fucI"
                     /locus_tag="PLI30_12010"
                     /EC_number="5.3.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009037509.1"
                     /note="catalyzes the conversion of the aldose L-fucose
                     into the corresponding ketose L-fuculose; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose isomerase"
                     /protein_id="HOI80314.1"
                     /translation="IGSVSMGIGGSMVNPDFLQDYLGMRTEQVDASEVLRRIQLEIYD
                     KEEFEKALAWTKENCMSREGEDFNPEHLKHSREQKDKDWEFVVKMTLVMRDLMIGNSK
                     LDEMGFGEEALGHNAIAGGFQGQRQWTDFLPDGDFSEAILNSSFDWNGKREAFTFATE
                     DDHLNGISMLFNHLLTNTSQMFADVRTYWSPEAIERVSGWKPDGLLKDGAIHLINSGS
                     CTLDGTGQQSDKDGNPVMKPFWEITDEEVSKMLEATTWHPASLEYMRGGGFSSQFLTK
                     PGMPVTMCRLNLIKGLGPVLQIAEGWTATFPAHVFDIINKRTDKTWPSTFFVPRITGK
                     GRFTDVYSVMNYWGA"
BASE COUNT          964 a          543 c          719 g          888 t
ORIGIN      
        1 accttcagac attcctgact gccggcccgc aggaggttcg tgcgtggaca tttcagaaag
       61 gatggaaagc accccaatgt gccggtgtta ttcacactga ttttgagaaa ggatttatcc
      121 gggcagaagt aataaaatac gcagactaca tacaatacgg ttcagaagcc accgttaagg
      181 aagccggaaa aatgaacgtg gaaggtaaag agtacatcgt tcaggacgga gatatcatgc
      241 atttcaggtt taacgtgtga tttttttatt taaaattatt ctaaattacg tttaaatgtt
      301 aataatatgt tgtaaaacat acaaataaat ttttaattaa tcgtttaacc ttatacattt
      361 gccttaatta atgttattat tttttcatca gttttgaaaa gagtctcttt aaaagatatt
      421 gccagagagt taggggtttc taccgcaacc gtttctcttg ttttgaatgg taaaaacatg
      481 tcaggccgtg taagcagtga gatgtcgcaa aaaatattgg ataaggcttc ggaattgaat
      541 tatattccca acacgttagc caagggatta aaagtaggca agtcacgaac catcggtttg
      601 atcattgctg atatttccaa cctttttttt ggtgcgttgg ccttacatat acaaatttat
      661 gccgagcaaa gagggtatag tgtgattata cgaaatacca atgagcagtt gaatgaaatg
      721 gagaatttaa tgaaatttct gtatgcccgg caagtggaag gactaattat tacgcccact
      781 gaaaatagtc agtatctttt gaaaagatta ataagcaata agattccgtt tgtgttggtt
      841 gacaggagct ttccagaatt gccggttaat tcggtgttga tcaataatta cgagatatct
      901 tatcgatcta ccgggcagct tataaagaaa gggtgtaaac gaattggtct gattacttat
      961 aaacaagatc attaccatat aaatgaacga aagcgcggta gtgtggatgc tatgaaacat
     1021 gcgggtattt acgatcctgg aatggtagaa gaagtccggt acgatttttt aaaaaatgac
     1081 atcaaaaggg ctatatgtaa tttaatatca aagaaagaaa aaacagatgg tttgtttttt
     1141 acaaccaatt caatttccat aaatggagta aaagagttga tcagaaacaa tatcaatatc
     1201 caaaaagaca ttcaaatcat gtgctttgac gaaaatgacg ctttttatat tttaccctac
     1261 gctgtacctt ttattaaaca acccatcaaa gagatggcaa aaaatgcggt agaattatta
     1321 atcgatcaaa ttgaaatgaa aagtgaagga actaaaaatg tcgttgtagg ggcagagtta
     1381 gtattaaacg aaaacatgat gtacctgtaa gcaattatac tcttattttt ttattaagtt
     1441 aaacgtttaa tcctattggt ttttatttga tgagtaaaat acttaccctt ttttccatta
     1501 agttaaacgt ttaatttaaa ttctaaaagt tttttatata tgaaacaaaa ttatcctaaa
     1561 attggcattc gcccgataat tgacggaaga cgcgggggaa tacgtgaatc gttggaagaa
     1621 acgaccatga atctggcaaa aagtgcggct gaattgtatt ccggaacatt gaaatatcct
     1681 gatggatcgc ctgtgaagtg tgtcattgcg gatacaacga tcggcggagt aaaggaagcg
     1741 gctttgtgtg ccgaaaaatt taaaaaagaa ggggtcggtt tgactttatc cgtgacgcct
     1801 tgctggtgct acggatctga aaccattgac atggatcctc ttatgccaaa agcagtttgg
     1861 ggattcaacg gcacggaacg ccctggcgcg gtttatttat cggcagcgct tgctgttcat
     1921 aaccagaaag ggctgcctgc ttttggaatt tacggcaaaa acgttcagga tgttggcgac
     1981 ggggcaatcc ccgatgatgt aaaagagaaa cttctccggt ttgctcgtgc cgggttggcg
     2041 gttgccatta tgcgtggaaa atcatacnnn nnnnnnnatt ggttcggtat caatgggaat
     2101 tggtggatcc atggtaaatc ccgatttttt gcaggattat ttaggcatgc gtactgaaca
     2161 ggtggatgcc agtgaagtcc ttcgtcgtat ccaacttgaa atttacgata aagaagaatt
     2221 tgagaaagcg ttggcctgga caaaagaaaa ctgtatgtcg cgtgaaggcg aagatttcaa
     2281 tccggaacac cttaaacaca gcagggaaca gaaagacaaa gactgggaat ttgttgtaaa
     2341 gatgacattg gtcatgcgtg atttaatgat tggaaattcc aaactcgatg aaatgggatt
     2401 cggggaagaa gcgctcgggc ataatgccat tgcaggaggc ttccagggac aacggcaatg
     2461 gactgatttc ttgccggacg gcgatttttc ggaagctatc ctaaactctt ctttcgactg
     2521 gaacggaaaa cgcgaagcgt tcacatttgc taccgaggac gatcatttga acggaatcag
     2581 tatgcttttc aatcatttgc taaccaatac ttcgcaaatg tttgccgacg tacgcaccta
     2641 ttggagtccc gaggccattg agcgagttag cggctggaag cccgatggat tgttgaaaga
     2701 cggggctatc cacctgatca actcgggctc ctgtacgttg gatggaaccg ggcaacaatc
     2761 ggataaggat ggaaatcccg taatgaagcc tttttgggag attaccgatg aagaagtctc
     2821 taaaatgctt gaagccacca cctggcatcc tgcatcgttg gaatatatgc gtggaggcgg
     2881 attttcctct caatttctga ccaaaccggg gatgccggtc accatgtgcc ggttgaattt
     2941 gataaaaggg ctgggtccgg tattgcagat tgccgaaggt tggacagcta cttttcccgc
     3001 tcatgtgttc gacattatca ataaaagaac ggacaaaacc tggccctcta ctttttttgt
     3061 tcctagaatt accggaaaag ggcgattcac agatgtttat tcggtaatga attattgggg
     3121 agcc
//
LOCUS       DAOSDR010000369         3122 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrimonas sp. isolate AMR_MDS_1826
            NODE_11028_length_3122_cov_3.190740, whole genome shotgun sequence.
ACCESSION   DAOSDR010000369 DAOSDR010000000
VERSION     DAOSDR010000369.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863355
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrimonas sp. (wastewater metagenome)
  ORGANISM  Petrimonas sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Dysgonomonadaceae; Petrimonas.
REFERENCE   1  (bases 1 to 3122)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3122)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:10:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,720
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 35
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3122
                     /organism="Petrimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11028_length_3122_cov_3.190740"
                     /isolate="AMR_MDS_1826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2023866"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1604
                     /locus_tag="PLI30_12015"
     CDS             <1..1604
                     /locus_tag="PLI30_12015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019538244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004560 - alpha-L-fucosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alpha-L-fucosidase"
                     /protein_id="HOI80315.1"
                     /translation="PQAYPISSHNADPLTEEHYRFNLNQVEEIMTNYGDISEIWFDMG
                     SLTLSQSQGLYELVSRLQPQCMISGRLGNDWVDFAVMADNEYPDYKLGVPWQTAASIF
                     PETWGYRSWQERGEVQPKVEEKIASLVKVISRGGNYLLNIGPRGDGSVVEFERDVLLG
                     IGKWIKVNAEAIYGTNANPFDQPFAWGSITTKGNDLFAFVENIPVSSSIRLSGFSGKV
                     SEVRLLASDESCRFSQKGNSVVINLPQRISGEFIPVLKIRFENGFKVVPSTVVTGNVL
                     SPQNATPVFGHSSLNYYGGYKSLIGYGWRVSSGKRAGSPELVYTDNERGRRLHIEIDG
                     KTQAVVLNGGSPRIEKLKKNTVKWGSLYRKPGRGVFGYVEEEGMAVVNVRAEDSGWEP
                     VSHFRYGEPYSEKIPPRQSMLFLQEIESEKDQSIAVEVGSGNGVYMLLNGAYLTAHLS
                     PWRVKFGKEIVLLPLQKGLNQLIIKHYNGYESDLSYSLQPLEEWSIYSQQLPVTRINQ
                     SVSIRAADAESKVAPLRMNNLRIIK"
     gene            1601..2590
                     /gene="dusB"
                     /locus_tag="PLI30_12020"
     CDS             1601..2590
                     /gene="dusB"
                     /locus_tag="PLI30_12020"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010801460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HOI80316.1"
                     /translation="MKIGNIAFTSRYPVFLAPMEDVTDIGFRLLCKQFGADMVYTEFV
                     SSDALIRHVRKTKEKLTICEEERPVAIQIYGNDPDSMVEAAKICEEANPDAIDINFGC
                     PAKKVAGRGAGSGMMKTPGLMLEITEKVVKAVGLPVTVKTRLGWDENSRIIVTLAEQL
                     QDCGIAALTIHGRTRSQKYTGEADWTLIGEVKNNPRMHIPIIGNGDLVTPQDCKRRFD
                     ETGVDAVMIGRASYGQPWIFSDVKHFLETGELAPKQSFSWYLDVLKQQVMQSVQRLDE
                     RRGILHIRRHLAGTPIFKGIPDFKPTRIALLRANTVEELFAVMDEIPEKFGIR"
     gene            2640..3005
                     /locus_tag="PLI30_12025"
     CDS             2640..3005
                     /locus_tag="PLI30_12025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI80317.1"
                     /translation="MKKLGLLLTALIAIAAIAPAQKTGIIADVLKKSAEEKVARMQEM
                     IGFDDNKARQLNELEFTFLLDVQKAENCCLCNRKKRIEKLKTAREEGLQKLLPRDEYM
                     KYHSIENELLRENNPLWLR"
BASE COUNT          830 a          717 c          887 g          688 t
ORIGIN      
        1 ttccgcaagc ttatcctatc tcgagccaca atgcggaccc gctcacggaa gaacactacc
       61 ggttcaacct gaaccaggtg gaggagatca tgacaaacta cggcgatatt tcggaaatct
      121 ggttcgatat gggctcactc acgctttcgc aaagccaagg attgtacgag ctggtcagcc
      181 gcctgcagcc gcagtgtatg atcagcggac ggttgggcaa cgattgggtg gattttgccg
      241 tgatggccga taacgaatat cccgattata agctcggcgt gccgtggcag accgccgcct
      301 ccatcttccc cgaaacgtgg ggctaccgct cgtggcagga gcgaggagag gttcagccga
      361 aagtggaaga gaagatcgcc agcctggtaa aggtgatcag tcgtggcggg aattacctgc
      421 tcaatatcgg ccccagaggt gacggatcgg tagtggagtt tgagcgcgat gtgctgctcg
      481 gtatcggtaa atggataaag gtgaatgccg aagccattta cggtacgaac gccaacccgt
      541 tcgaccagcc attcgcctgg ggcagcataa ccacgaaggg caacgacctg tttgcttttg
      601 tggaaaatat tcctgtgtcg tccagcatac gtttgtcggg cttttccgga aaggtgagcg
      661 aggtacgcct gctggcgtcg gacgaatcct gccggttttc ccaaaaagga aactcggttg
      721 tgatcaattt gccacaaagg atctccgggg aatttatccc tgttttaaag atccggtttg
      781 aaaacggatt taaagttgtc ccttcaaccg ttgtaaccgg taatgtgctt tcgccacaaa
      841 acgccacacc cgtattcggc cattccagct taaactatta cggtggatat aagagcctga
      901 tcgggtacgg ctggagggtg tcctcaggca aaagagcggg gagcccggag cttgtgtata
      961 cggacaatga acgggggcgt cgtctccata tcgagatcga cggaaagaca caagccgttg
     1021 tgcttaacgg cggcagcccc aggatcgaaa agctgaaaaa gaacacggtg aagtggggca
     1081 gtttatacag aaaacccgga cgcggcgtgt ttggatatgt ggaagaggag ggtatggctg
     1141 tcgtgaatgt ccgtgcggag gattcgggct gggaacccgt cagtcatttc cggtacggtg
     1201 agccctactc cgaaaaaatt ccgccgcgtc aaagcatgtt gttcctgcaa gagattgaat
     1261 cagaaaaaga tcagtccata gcggtggagg taggcagtgg aaacggggta tatatgttgc
     1321 tcaacggagc atacctcact gcccatttat ctccctggcg cgtaaaattc gggaaggaga
     1381 ttgtccttct tcccctccag aaaggcctga atcaattgat tataaaacac tataacggat
     1441 acgagagtga cctgagttac agtttgcaac ctctcgaaga atggtcgatc tactcccaac
     1501 aacttccggt aacacggata aatcaatcgg tttccatacg tgctgcggat gcggagtcga
     1561 aagtggcacc gttgaggatg aacaatttaa ggattataaa atgaaaatag gaaacatagc
     1621 atttacatcg cggtatcccg tttttctggc accgatggag gatgttaccg atattggttt
     1681 tcggttgctg tgcaagcagt tcggagcaga tatggtatat accgagtttg tgtcgagcga
     1741 cgcacttatc cgccacgtga ggaaaaccaa ggaaaagctt acgatctgtg aggaagagcg
     1801 tcccgtagct atacagattt acggaaacga tcccgattca atggtcgagg ctgcgaagat
     1861 ctgcgaggag gcaaaccccg atgccatcga tatcaacttc ggatgtccgg ccaagaaggt
     1921 ggccgggaga ggcgccggtt cgggaatgat gaaaacaccg ggtttgatgc ttgagatcac
     1981 agaaaaggtg gtgaaggctg tcggcctgcc cgtgactgtg aagacccggt tagggtggga
     2041 cgagaactcc agaatcattg ttacgctggc agagcagctg caggattgcg gaattgccgc
     2101 actcaccatt cacggaagga cacgctctca aaaatatacc ggagaggccg actggaccct
     2161 tatcggcgag gttaaaaaca atccccgtat gcacattccc atcataggaa acggtgatct
     2221 ggtaacgccg caggactgta aacgccgttt cgacgaaacc ggcgtggatg cggtgatgat
     2281 cgggcgggcg agttacgggc agccgtggat cttcagtgat gtaaagcatt ttctcgaaac
     2341 gggggagctg gctcctaaac aatctttttc ctggtacctc gatgtgttga agcaacaggt
     2401 aatgcagagt gtgcagcgac tggatgagcg gcgtggtatt ctccatatcc gccgccatct
     2461 ggcaggaacc cccatcttta agggtatccc cgattttaaa cccacccgca tagcgctgct
     2521 ccgggcgaat accgtggagg agttgtttgc cgtgatggat gaaattcctg aaaagttcgg
     2581 gatacgttaa acaaacagcc ttcgggatag tcagattact aacactaaaa aaggacgaga
     2641 tgaaaaaatt gggattgttg ctgaccgctt tgattgcaat tgctgccatc gccccggcac
     2701 aaaaaacggg aataattgcc gacgtgctga aaaagagcgc cgaagagaaa gtggccagga
     2761 tgcaggagat gatcggtttt gacgataaca aggccaggca gctcaatgag ctggagttta
     2821 cgtttctgct cgatgtccag aaagcggaga actgctgcct gtgcaacagg aaaaaacgga
     2881 tcgagaaatt gaaaacagca cgggaagagg gcttacaaaa gttgttgccc cgcgatgagt
     2941 acatgaagta ccattccatt gaaaacgagt tgttgcgcga gaacaacccg ctctggttgc
     3001 ggtaaaacag caggaaggaa tccttatttc aggggtggaa gcaacttcgg atcatcaata
     3061 atcactgcct gtctgctaac cgattcgatg gttgagtaga gctcagcatc cttatattcc
     3121 gg
//
LOCUS       DAOSDR010000370         3121 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrimonas sp. isolate AMR_MDS_1826
            NODE_11031_length_3121_cov_4.065884, whole genome shotgun sequence.
ACCESSION   DAOSDR010000370 DAOSDR010000000
VERSION     DAOSDR010000370.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863355
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrimonas sp. (wastewater metagenome)
  ORGANISM  Petrimonas sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Dysgonomonadaceae; Petrimonas.
REFERENCE   1  (bases 1 to 3121)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3121)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:10:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,720
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 35
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3121
                     /organism="Petrimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11031_length_3121_cov_4.065884"
                     /isolate="AMR_MDS_1826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2023866"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(85..1956)
                     /locus_tag="PLI30_12030"
     CDS             complement(85..1956)
                     /locus_tag="PLI30_12030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006843366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HOI80318.1"
                     /translation="MANPLLQIDTLTKSFGDRMLFRNISFGVAEGDKIGLIAGNGAGK
                     TSLLNIIAGKEPSDNGRVVFRNDIRFAYLEQSPQFDPGLTVLEACFQTENEAVKVIAR
                     YEEIMESGNHLELDEILAKMDLLKAWDYEQRIRQILSQLKIFQLDQKIGELSGGQVKR
                     VALANTLISEPELIMLDEPTNHLDLDMVEWLEEYLSRSNISLLMVTHDRYFLDRVCTQ
                     IIEIDDRQIYSYKGNYAYYLKKRGERIVVQNAEADSAQNIYRKELDWMRRQPQARATK
                     AKSRIDAFYELEEQVKQRNQANDIKLSAKGSYIGKKIFEANHITKSFGDIKITDDFNY
                     IFTRYEKMGIIGNNGTGKSTFIKMLLGIEQPDSGNFDIGETVNFGYYSQEGLQFDEQT
                     KVIDAVQKIAEVIDLGNGNKLTASQFLQHFLFAPAKQHDFVYKLSGGEKRRLHLCTVL
                     IKNPNFLILDEPTNDLDIITLNILEEYLIGFKGCLIVISHDRYFMDKVVDHLLCFHGD
                     AEIRDFPGNYTQYRDWKSLEDKRVAEETKPPKPIKTSEKPVQQEKTKLTFREKQEFEG
                     LEAEIQRLETEKEQLAQQLSSGDLSSEELINASNRISQVVSLIDEKTYRWLELSEFA"
     gene            complement(2059..>3121)
                     /locus_tag="PLI30_12035"
     CDS             complement(2059..>3121)
                     /locus_tag="PLI30_12035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI80319.1"
                     /translation="EKKNNSREQVTGVNINYHNEQLQLGVSGVYHAYSRMYCPVVREY
                     NYYSLRDSSHFNASIDYSYRLNRLAFAGETAVARNGATATTHMIQYTPSGLFSLSALY
                     RHFPISYNAMHAQAFSEGSRVQNENGFYLGVTFSPLQKVSATTYIDLFRFPWPKDQVD
                     KPSAGFDFYFLGTYAMDRNSNFELRYKIKQKEQNTDYPENSRKVLPYNTQKIRLRYNH
                     ALNNGWDFRTTLDAALFRRKFFPLEKGFMLSQHAGYRGGKKIRGDLFAGYFKSDTYAT
                     RLYSYERNLLTTFYMPSFHGKGIRLAASGRYAFTPRLSFSVKIGHTRYFDRETIGSGT
                     ERISGNRRTDLFTYWQWQF"
BASE COUNT          688 a          710 c          792 g          931 t
ORIGIN      
        1 gacataaccg gctgtgagaa tttctactcc gaagaagttc ctcttttttc tgaaggatgt
       61 atagtttttc tctggcataa gaagtcaggc gaattcgctc agctccagcc agcgataggt
      121 cttttcgtca atcaggctga cgacttgcga tatgcggttt gatgcgttga ttagctcttc
      181 tgacgagaga tcccccgatg agagttgttg tgccaattgt tctttttccg tttcaaggcg
      241 ttggatttct gcctccaacc cctcaaattc ctgtttttcc ctgaacgtga gtttggtctt
      301 ctcttgttgg acaggttttt ctgaagtttt tatcggcttt ggtggttttg tttcttcggc
      361 aacccgtttg tcttccaatg atttccagtc gcggtattgt gtgtagttcc ccggaaaatc
      421 ccggatctcg gcgtctccgt gaaaacagag caggtgatcc accaccttgt ccataaaata
      481 gcggtcgtgg gaaataacga tcaggcagcc tttaaatccg atgaggtatt cttccagtat
      541 gttgagtgta atgatatcga gatcatttgt aggctcgtca aggatcagga agttggggtt
      601 tttgataagc acggtacaga ggtgcagtcg gcgtttttca ccaccgctca gcttgtagac
      661 gaaatcgtgt tgcttggccg gagcaaagag aaaatgctgc aggaactgtg atgccgtgag
      721 tttgttccca ttgcccagat ctatgacttc ggctattttc tggacggcgt caatgacctt
      781 ggtctgttca tcaaactgca gcccttcctg actgtaatac ccgaagttca ccgtttctcc
      841 gatatcgaag ttgcctgaat cgggttgttc gatccccagt aacattttta tgaacgtcga
      901 tttgccggtg ccgttgttac cgataatccc cattttttcg taccgggtga aaatatagtt
      961 gaagtcgtcg gtaatcttaa tgtcgccaaa acttttggta atgtggttgg cttcgaatat
     1021 ttttttgccg atatacgaac ctttcgccga gagttttatg tcgttggctt ggttgcgttg
     1081 tttgacctgc tcttccagtt cgtaaaacgc gtcaatgcgc gattttgctt tggtagcacg
     1141 ggcctgcggt tgccggcgca tccagtcgag ctctttgcgg tagatgtttt gtgcgctgtc
     1201 agcttcggca ttctgtacga cgatgcgttc accgcgcttt ttcaggtaat aggcataatt
     1261 gcctttgtag gagtagattt gacgatcgtc aatttcgatg atttgcgtac ataccctatc
     1321 gagaaaatag cggtcgtgag taaccatcag taaactgatg ttcgagcggg aaagatactc
     1381 ctccagccat tccaccatat ccagatcgag gtggttggtc ggttcgtcca gcatgatcag
     1441 ttccggctcc gagataaggg tgtttgccag cgctacccgc ttgacttgcc cgccggaaag
     1501 ttcgccgatt ttctgatcga gctgaaagat cttcagctgg ctgagaatct gccggatgcg
     1561 ctgttcgtag tcccaggctt tgagcaaatc catttttgct aaaatttcat ccagctccag
     1621 atgattgccg gattccatga tctcttcgta tcgcgcaatt actttcaccg cttcgttttc
     1681 ggtttgaaaa caagcttcga gtaccgtgag tccgggatca aactgcggcg attgttccag
     1741 gtaagcaaac cggatatcgt ttcggaaaac aactctccca ttatcgctgg gttcctttcc
     1801 ggcgatgatg ttcagcaatg aggtctttcc cgcgccgttt cctgcaatca gaccgatttt
     1861 gtcgccttcg gcaaccccga aagagatgtt gcggaaaagc atccgatcgc cgaagctttt
     1921 ggttagcgta tcgatttgca ataatggatt tgccatagtt gagtaaattt tgcctttggc
     1981 gatcgtcacg ttagtgtgga ccctgtattt tgtgcgataa acgccggtac aaaagtacga
     2041 aactatttgg gattaaactc aaaattgcca ttgccaataa gtaaacaggt ctgttcggcg
     2101 gttgccgctg atccgctctg ttcccgagcc tatggtttca cggtcgaaat aacgtgtatg
     2161 cccgatcttt acggagaatg acagccgggg ggtgaaagca tatctgcctg atgctgcaag
     2221 ccttatgcct ttgccgtgga acgacggcat ataaaaggtc gtcaggaggt ttctttcgta
     2281 agaatagagc ctggtggcat aggtgtctga tttaaaatat ccggcgaaga ggtcgccccg
     2341 gatctttttg ccgccgcgat agccggcatg ttgagagagc ataaaccctt tttccagcgg
     2401 aaaaaatttc ctgcggaaca aagcggcatc taaagtagtc cggaagtccc acccgttgtt
     2461 tagtgcgtga ttatatcgga gcctgatctt ttgtgtgttg tagggtaata cttttctgga
     2521 gttttcggga tagtcggtgt tctgctcttt ctgttttatt ttgtaccgca gctcgaagtt
     2581 ggagtttcga tccatggcgt acgtacctaa aaaatagaag tcgaagcctg ccgacggctt
     2641 atctacctgg tctttcggcc aggggaaacg gaaaaggtca atatacgttg ttgccgatac
     2701 cttttgcagc ggactgaagg tgacgcctaa atagaatccg ttctcgtttt gaacccggct
     2761 cccttcggaa aatgcctgcg catgcattgc gttgtaagaa atggggaaat ggcggtaaag
     2821 tgccgacagg gagaaaagcc cggacggtgt atattgaatc atgtgggtgg ttgctgtggc
     2881 tccgtttctg gccactgcgg tttccccggc aaacgccaac cggtttaagc ggtacgagta
     2941 gtcaatgctt gcattgaaat gggacgagtc ccgcaaactg taatagttgt attcgcgaac
     3001 aaccgggcaa tacatacggc tataggcgtg atatacaccg ctcacgccta actgaagctg
     3061 ctcgttgtgg tagttgatat tcacccccgt tacctgttca cgcgaattgt tttttttctc
     3121 t
//
LOCUS       DAOSDR010000371         3115 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrimonas sp. isolate AMR_MDS_1826
            NODE_11075_length_3115_cov_3.500327, whole genome shotgun sequence.
ACCESSION   DAOSDR010000371 DAOSDR010000000
VERSION     DAOSDR010000371.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863355
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrimonas sp. (wastewater metagenome)
  ORGANISM  Petrimonas sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Dysgonomonadaceae; Petrimonas.
REFERENCE   1  (bases 1 to 3115)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3115)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:10:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,720
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 35
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3115
                     /organism="Petrimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11075_length_3115_cov_3.500327"
                     /isolate="AMR_MDS_1826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2023866"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(478..2226)
                     /locus_tag="PLI30_12040"
     CDS             complement(478..2226)
                     /locus_tag="PLI30_12040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006841560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOI80320.1"
                     /translation="MSFRLSYRKRILLYFSVIIAVFTAGIILFEQKQVRHERTKSLEN
                     MLDGNAGFIHRYIKGRHIVFPDSIQRMDELTAYLSDNLRLTIIDKTGRVMYDNLLDEK
                     RMTNHTDRPEIQKAMVSGSGSNIRMSDSNHIRYLYYARNYDGAYFIRVALPYEVEVRP
                     FLNSKNSFMYFIILFFIVCALMMFYFANKFSKSIKELKEFAVNLKNGRPTKTAFEFPD
                     DEVGEVSAEIVENYNLLKENRKKLALEREKLIQHFQFSEEGIAIFSEERKKIYANSHF
                     LQFLNIIADKPVLEDEYLFSDPDFEEIDAFLASADRTENVFSTRIEKNGKQFNIRVII
                     FEDKNFEIYISDITKAEKRRLLKQEMTNNIAHELRTPVTSIRGYLETILNLDPKDTDR
                     QQVFIDRAYVQTIRLSELIQDISMLTKIEEAADRFVKEPVFIKLLLDELKSDLAHIFL
                     EKNTTFTIEVADSVVIYGSRTLLYSIFRNLVENSLAYAGKSIQIVLRCYMETDDAVYF
                     EFHDNGVGVEEKHLVKIFERFYRVNEGRTRDTGGSGLGLSIVKNAVLLHKGNIVAKNR
                     PEGGLLFLITFPKIKA"
     gene            complement(2223..2915)
                     /locus_tag="PLI30_12045"
     CDS             complement(2223..2915)
                     /locus_tag="PLI30_12045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019540745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOI80321.1"
                     /translation="MEERILVVDDEKDICDILQFNLENEGYEVDLANSAEEALSSLTD
                     KHDLIILDVMMGGMSGFKMAERLRKDGKSVPIIFLTAKNTENDMLTGFSLGGDDYVSK
                     PFSVKELIARVKSVLKRVRLNKKETQTLWQHEGLTIDILNNRVTIDDVEVTLTKKEFE
                     ILSLLSQTSPNVYTRSEILNQVWGDNEFVLDRTVDVHITRLRKKLGEYSSIIVNRSGF
                     GYYLNSEPKTTE"
     gene            complement(2934..>3115)
                     /locus_tag="PLI30_12050"
     CDS             complement(2934..>3115)
                     /locus_tag="PLI30_12050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphate uptake regulator PhoU"
                     /protein_id="HOI80322.1"
                     /translation="YQMLINIVNLNNIAYYIERIGDKAVDMAESAIFLLEGKDVRHDK
                     ELKKSLPFKAGEETQ"
BASE COUNT          893 a          656 c          549 g         1017 t
ORIGIN      
        1 cgggaacttg gcctcttttc accatgtaat tgactttttc gtcgaaatta cggatattgc
       61 acatcaactt gtgcatgtgc agcaattttt tcttgggtat ttcttgttta gctgttgatt
      121 ttttagattt tgccataata tatttagttg taatattgga tcaagcccat ctcgtattac
      181 ctgaatcagt aacaataaca ttttttgaat gatatttgtt taacacatat cattttttta
      241 tcgatgataa agcgggttga ttgaaatcct gattctgtga tttttctaca aagttattta
      301 gaattgtttt aaattacaaa accgtttagt ttatttaacc attgcgtttc tatttttcgg
      361 ttaaaataag cgttgccagt caaaagtatt attgcttccc ccacagataa gggtagtgtc
      421 aaacttgttc ggattgtgtt aagacgagta ataatcgaat aaaatttatt gttgcagtta
      481 tgctttgatt tttggaaaag ttatgagaaa aagcaaccca ccctcggggc gatttttggc
      541 tacgatgtta cccttgtgta aaagcactgc atttttaacg atagacaaac ctaagcctga
      601 accgccggta tcacgagtac gtccttcgtt caccctgtaa aaccgttcaa aaatctttac
      661 gaggtgtttc tcctccaccc caactccgtt atcgtgaaat tcgaagtaca cagcatcatc
      721 agtttccatg taacacctaa gcacaatctg aatagacttg ccagcatatg ctaaggagtt
      781 ttccacaagg tttcggaaaa tggaatacag gagtgtgcgg ctgccataaa taaccacact
      841 atccgctacc tcgatagtaa atgtggtgtt tttttcaagg aatatatggg ctaaatcaga
      901 tttcaattca tcaagaagca attttataaa aacgggttct ttaacaaacc ggtccgcagc
      961 ctcttctatt ttagtcaaca tgcttatatc ctgtattaat tcggataacc gaatggtttg
     1021 aacatacgct cgatcaataa aaacctgctg acggtctgta tctttggggt caaggttaag
     1081 tatggtctcc agataaccac ggatactggt aaccggtgtc cggagttcat gagcgatatt
     1141 gttggtcatc tcctgcttga gcaagcgtcg cttttcagcc ttagtaatgt cgctgatata
     1201 aatttcaaaa tttttatctt cgaaaataat gaccctgata ttgaactgct taccgttttt
     1261 ttctatacgc gtcgagaaca cattttcagt acggtcggcc gaagctagaa aagcatcaat
     1321 ctcttcaaaa tcaggatcgg aaaataaata ttcatcttcc aacacgggtt tatctgctat
     1381 aatattcaaa aattgcagaa aatgggagtt ggcgtagatt ttcttccgtt cttcggaaaa
     1441 aatggcaatc ccctcttcgg aaaattgaaa atgctgaata agtttttctc gctccaaagc
     1501 cagttttttc cggttttctt tcaataaatt gtaattctcc acgatctcgg cactaacctc
     1561 tcctacctca tcgtcgggaa actcgaacgc tgtttttgta ggcctaccat tcttcaggtt
     1621 taccgcaaat tcctttaact ctttaattga tttggaaaat ttattggcaa aatagaacat
     1681 catcagcgca catacaatga aaaacaaaat gatgaaatac atgaaactgt ttttggaatt
     1741 aagaaacggc ctcacttcca cctcgtaggg caaggcaacg cgaataaaat atgctccgtc
     1801 gtaattcctg gcataatata aataccggat atggttactg tccgacattc gtatgttgga
     1861 accgctacct gaaaccattg ccttttgaat ttcgggacgg tctgtatgat tagtcatcct
     1921 cttctcgtcc agcagattat catacattac tctgcccgtc ttatcaataa tagtcaaacg
     1981 taggttatcg gataaatacg ctgttaattc atccatccgt tgtatgctat ccgggaatac
     2041 aatatgccgg ccttttatgt agcggtgaat aaaaccggca tttccgtcaa gcatattttc
     2101 aagacttttc gtacgttcgt gccggacttg cttttgttca aacaaaataa tccccgccgt
     2161 aaaaactgca atgattacgg agaagtacag caagattctt tttcggtatg acagccggaa
     2221 actcattctg tcgtttttgg ctctgaattc aggtaatatc cgaatcctga acgatttaca
     2281 ataattgatg aatattcgcc taattttttc ctgaggcgag ttatgtgcac atctactgtt
     2341 ctgtcgagaa caaactcgtt gtcaccccaa acctgattca agatttcgct gcgggtataa
     2401 acgttcgggg aggtttgcga caacaacgaa agaatttcaa actctttttt cgtgagtgta
     2461 acttcaacat catctatggt caccctgtta ttaagaatat caatggttaa tccctcatgc
     2521 tgccagagtg tctgcgtctc tttcttattt aaacggactc tttttaaaac cgattttacc
     2581 cttgcaatca gttcttttac agaaaaaggt ttagacacat aatcatcacc gcctaaggag
     2641 aagccggtca gcatatcgtt ctccgtattt tttgccgtca gaaatatgat gggaacgctc
     2701 tttccatctt ttctgagcct ctcggccatt ttaaaacccg acattccgcc catcataaca
     2761 tccagaataa tcagatcgtg cttgtcggta aggctcgaca atgcctcctc agccgaattg
     2821 gccaaatcta cttcataccc ttcgttttcc aaattgaact gaagaatatc gcaaatgtct
     2881 ttttcatcgt ctacaaccag aattctttct tccataactg ataaaattga tgtttattga
     2941 gtttcctccc cagctttaaa aggcaatgac ttttttaatt ctttatcgtg tcgaacgtct
     3001 tttccttcca acagaaaaat ggcagattcc gccatatcta ctgctttatc gccgatacgc
     3061 tcaatgtaat aagcaatatt gttaagattt acaatattaa taagcatttg ataac
//
LOCUS       DAOSDR010000372         3089 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrimonas sp. isolate AMR_MDS_1826
            NODE_11202_length_3089_cov_3.922544, whole genome shotgun sequence.
ACCESSION   DAOSDR010000372 DAOSDR010000000
VERSION     DAOSDR010000372.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863355
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrimonas sp. (wastewater metagenome)
  ORGANISM  Petrimonas sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Dysgonomonadaceae; Petrimonas.
REFERENCE   1  (bases 1 to 3089)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3089)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:10:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,720
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 35
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3089
                     /organism="Petrimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11202_length_3089_cov_3.922544"
                     /isolate="AMR_MDS_1826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2023866"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(245..1693)
                     /locus_tag="PLI30_12055"
     CDS             complement(245..1693)
                     /locus_tag="PLI30_12055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019539751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophanase"
                     /protein_id="HOI80323.1"
                     /translation="MQEIKFYNGENIPLELHKVRVVQKLHLVPIERRLEALEEGGFNT
                     FRLNTKDVFLDMLTDSGTDAMSDNQMAAMMQADDAYAGSQSFYRLKKAVEDVLGKKHY
                     LPVHQGRAAENILSKAYIKQGSLIPMNYHFTTTLAHITECGGRIVELLYDEGYVMNSD
                     HPFKGNMNIEKLEENIIRHGSKNVPFIRMEASTNLIGGQPFSIQNLRDVRAIADKYNI
                     RLVLDASLLGENAYLVKLREEEFNETSMADILKTMTELADIVYFSARKLSSSRGGGIC
                     TNSEVIYKELEALVPLYEGFLTYGGISVREIESMAVGLYETLDETMICQSPDFIRYLV
                     DELDKNGVPMLKPAGVLGAHVNAMELCSHIPQTQYPAGSLAAALFLISGIRGMERGSV
                     SNQRDEYGNETYADMELVRLAVPRRVFTLSQIKYVIDRVTWLNDNRELIGGLKFVYEP
                     PVLRFFMGGLEPVNDWPQRLISKFKEDFGESL"
     gene            1953..2915
                     /locus_tag="PLI30_12060"
     CDS             1953..2915
                     /locus_tag="PLI30_12060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008152519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HOI80324.1"
                     /translation="MDKPYVIGIDVGGTNTVVGIVDKRGQILRSGSLKTAKHAEVEDY
                     LNELSQVIEDVIKDIATKDQIKGIGAGTPNGNYFTGSIEFAPNLRWKGVIPFAQMLED
                     RVGIPVALTNDANAAAIGEMTYGAARGMKDFIVITLGTGVGSGIVVNGQMVYGHDGFA
                     GELGHVIMRRTNGRVCGCGRSGCLEAYTSATGVARTAREYLELQHDAKTQLRNIPIDD
                     ITSKDVFDAAMAGDEMAKEIFRFTGEMLGEAFADFVAFSSPEAIILFGGLAKAGDLIM
                     NPIRESMERNLLPIFKNKVKLIFSELKESDAAVLGASALGWEVR"
BASE COUNT          891 a          642 c          676 g          880 t
ORIGIN      
        1 gaatgtaggt caagtatatt tcttcaatac accattgaca gtgttttcaa taggataaaa
       61 tctaactgaa aattggataa aacggtattg ttgtgtttaa ctaagggttg ggtatcggca
      121 attatttaac caaataaaac cccgccttga agagtttgtg aaattataaa gattaattac
      181 aaattgaaaa tctttttaca gttttcatac agtctccaag acggggaatt tattcagcgc
      241 gttatcacaa gctttctcca aaatcttcct tgaatttgga aataagcctt tgtggccaat
      301 cgttaacagg ctccagccct cccataaaga aacgaagaac aggcggttcg taaacgaatt
      361 ttaatccacc gataagttca cggttgtcat tcagccaggt aacccggtcg ataacgtatt
      421 ttatctgtga taacgtgaac acacgacgtg gaacggcaag ccggactaac tccatgtcgg
      481 cgtatgtttc gtttccatac tcgtcgcgtt ggttggaaac cgagccgcgt tccattcccc
      541 ggattccgga tatcagaaac aaggctgcag caagcgatcc ggcaggatac tgcgtttgag
      601 gaatatggga acagagttcc atagcattca cgtgtgcacc gagtacgccg gcaggcttca
      661 gcatgggtac tccgttttta tccagttcat ccaccaggta acggataaag tcgggacttt
      721 gacaaatcat ggtttcatcc aacgtttcgt ataatccgac tgccattgat tcaatttcac
      781 gtaccgatat gccaccgtaa gtcaaaaatc cttcataaag gggaacaagt gcctcgagct
      841 ccttgtaaat cacttcgctg ttggtgcaga tacctccacc gcgcgaagaa cttagcttcc
      901 gtgccgaaaa atagacaata tctgccagct cggtcattgt ttttagaata tccgccatag
      961 acgtttcatt aaactcttct tcccgcaatt ttaccaggta agcattttca cccaataagc
     1021 tggcatccag cactaaacgg atattgtatt tatctgcaat agccctcaca tcgcgtaggt
     1081 tttgaatcga aaatggttgc ccgccaatca ggttggtgga agcttccatg cggataaacg
     1141 gaacgttttt tgaaccgtgc cggataatgt tctcttccag tttttcgatg ttcatgttgc
     1201 ctttaaacgg atgatcagaa ttcatcacat acccttcatc gtaaagcagt tccacgatac
     1261 ggccaccaca ctcggttata tgggccaaag tagtggtgaa gtgatagttc atcggaatca
     1321 ggctcccttg cttgatgtat gcttttgata aaatattttc ggctgcacgg ccttgatgaa
     1381 ccggcaggta atgttttttc cccagaacgt cttctacagc tttctttaac cggtaaaaac
     1441 tttgtgatcc ggcatatgca tcatctgcct gcatcatcgc agccatctga ttgtcgctca
     1501 tagcatcggt tccgctgtcg gtaagcatgt ccagaaatac atctttcgtg tttagtctga
     1561 aagtgttaaa tccgccttct tccaatgctt ccagtcgacg ttcaataggt actaaatgta
     1621 gtttctgcac aacacgcact ttgtgcaatt ccaacgggat attctctccg ttataaaatt
     1681 taatttcctg cataataatt gtttattttg ttttgtaaag ataaaagtct gaattcctct
     1741 aatcggtcaa aatctaatgt gaaaaactgt cgttttggca aaacgatctg caaagatatg
     1801 tcaaattata aaatcattac ccattatttg agtttaattt aaaaaaaggg ggttgcttgt
     1861 aattttttta accatttcaa gtaaggatga caaattaatc gtatatttgc gagtacaatt
     1921 aatctcattg aataacattt aaacagaaac tcatggataa accttatgta ataggtatag
     1981 atgtcggcgg tacaaatacg gttgtgggta tcgttgataa gaggggacag attttacgta
     2041 gcggaagtct gaaaacggct aagcatgctg aagtggaaga ctacttgaat gaattgagcc
     2101 aagtgattga agatgtgatc aaggatattg ccaccaaaga tcaaatcaaa ggaattggag
     2161 ccggaacacc caacggcaac tatttcaccg gaagtatcga atttgctccc aaccttcgtt
     2221 ggaaaggagt tattccgttt gcacaaatgc tggaagacag agtaggtatt cccgtggcgc
     2281 ttaccaacga tgccaatgcg gcagccatcg gtgaaatgac atacggggca gcccgcggaa
     2341 tgaaagactt tattgtaatc actctcggaa ccggtgtagg aagtgggatt gtggtgaacg
     2401 gacaaatggt gtacggccac gatggctttg caggtgaact tgggcatgta atcatgcggc
     2461 gcacgaacgg aagagtgtgt ggttgcggaa gatcgggatg cttggaggca tatacctcag
     2521 caacgggagt agcccgtact gcacgtgaat acctggagct tcaacacgac gcaaaaactc
     2581 agttgcgtaa catccccatt gatgacatca cttccaaaga tgtctttgat gcggctatgg
     2641 caggcgatga aatggccaaa gaaatattcc gttttaccgg tgaaatgtta ggtgaagcat
     2701 ttgctgattt tgtagcgttc tcaagccctg aagccattat cctttttgga ggattggcga
     2761 aagccggtga tctcattatg aatccgatcc gcgaatcaat ggaaagaaac ctgttgccca
     2821 ttttcaaaaa caaagtgaaa ttgatcttct ctgaactcaa ggaaagtgat gcggcggtac
     2881 ttggcgccag cgcactgggt tgggaagtga gatgatttta tacagactac agattataaa
     2941 ccgtctcagg aatgaggcgg tttttttctt ttgttttcat ccttcatggc tctgcaacga
     3001 atcatttttg tgaaagagcc gagatttatt tacatttgca taagatctat atctatacag
     3061 aacgatacaa ctaacgatct cgaaaaaat
//
LOCUS       DAOSDR010000373         3086 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrimonas sp. isolate AMR_MDS_1826
            NODE_11216_length_3086_cov_4.976575, whole genome shotgun sequence.
ACCESSION   DAOSDR010000373 DAOSDR010000000
VERSION     DAOSDR010000373.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863355
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrimonas sp. (wastewater metagenome)
  ORGANISM  Petrimonas sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Dysgonomonadaceae; Petrimonas.
REFERENCE   1  (bases 1 to 3086)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3086)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:10:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,720
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 35
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3086
                     /organism="Petrimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11216_length_3086_cov_4.976575"
                     /isolate="AMR_MDS_1826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2023866"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..457
                     /locus_tag="PLI30_12065"
     CDS             <1..457
                     /locus_tag="PLI30_12065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI80325.1"
                     /translation="GDYFGNAQERYEMLFQSYPVTKSDLDSIRLTVDGFFTALSALKA
                     NEITKYMAPVVFRFFNSGGGQREKIVGDLIISGARTQAPTVQFLPNTAAVAYEKTDIE
                     HYKVNVPVQKSYVDGGQQKTVSGYIVQMELDRNFRILTLTERKPSADAV"
     gene            complement(463..1488)
                     /gene="tdh"
                     /locus_tag="PLI30_12070"
     CDS             complement(463..1488)
                     /gene="tdh"
                     /locus_tag="PLI30_12070"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008743 - L-threonine 3-dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HOI80326.1"
                     /translation="MKALVKLRPEKGIWMQEIPVPEIGVNDVLIKIIKTSICGTDLHI
                     YKWDDWAQKTIKTPMTIGHEYVGEIVDMGSGVKNLKTGDRVTGEGHIACGHCRNCRRG
                     KLHVCENTLGVGVNRDGAFAEYLSLPAENVVKLDPRIPDEIASIMDPFGNATHTALSF
                     PLIGEDVLITGAGLIGSMATAICRFAGARYVVVSDLSDYRLEIARKMGATLTVNPSKG
                     ESIRQAVEKLGMRGFDVGLEMSGSPAAFREMIDNMYNGSKISLLGILPNNTTVDWNEI
                     IFKALTLKGIYGREMWETWYQMEQMLISGIDLTPVITHRFSIDEYQKGFEVMDSGQCG
                     KVILSWD"
     gene            complement(1515..2705)
                     /gene="kbl"
                     /locus_tag="PLI30_12075"
     CDS             complement(1515..2705)
                     /gene="kbl"
                     /locus_tag="PLI30_12075"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010802868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008890 - glycine C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HOI80327.1"
                     /translation="MYEKMQQHLQSELAAIQEAGLYKNERIIVTPQKAEIKVKSGQEV
                     LNFCANNYLGLSDNAHLIEAAKKALDERGYGMSSVRFICGTQDLHKELEATISKFFKT
                     EDTILYAACFDANGGLFEPLFTEEDAIVSDALNHASIIDGVRLCKAKRYRYANADMAD
                     LEAKLQEAQAQRFRIIATDGVFSMDGNVTPIDKIMELAEKYDALVMIDESHSAGVVGK
                     TGRGVTEQYNCLGKAEIITGTLGKAFGGAIGGFTTGRKEIIDMLRQRSRPYLFSNSIP
                     PLVAAAGIKAFELLQESNELQDKLHENVDYFVAKMKEANFDIKPTQSAICAVMLYDAK
                     LSQEFASALLDEGIYVTGFYYPVVPKGEARIRVQLSAGHKREHLDKAIAAFIKVGKKL
                     AVIK"
     gene            complement(2745..>3086)
                     /gene="rmuC"
                     /locus_tag="PLI30_12080"
     CDS             complement(2745..>3086)
                     /gene="rmuC"
                     /locus_tag="PLI30_12080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019540668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HOI80328.1"
                     /translation="DIWQYAYDKKILLISPTNLIAALKLIVDLWKREYQNRNAIEIAE
                     RGAKLYDKFVGFINNLEKIGKNLDQAQNAYNDAYKQLSTGNDNLVLQTQKLKDLGVKA
                     KKSLPQSLIEE"
BASE COUNT          692 a          751 c          765 g          878 t
ORIGIN      
        1 gggcgactat ttcggcaatg cacaggaaag atacgaaatg ttgttccaat cgtatccggt
       61 tacaaaaagt gatctggaca gtatccggct taccgtcgac gggttcttta cggcattatc
      121 cgctcttaaa gccaacgaaa taaccaaata tatggctccg gtcgttttcc ggttcttcaa
      181 ctccggtgga ggtcaacgtg aaaaaatagt tggagacctg attatctctg gggcgagaac
      241 acaggctcca accgttcagt ttttaccgaa tacggccgca gtggcatacg agaaaacgga
      301 tattgaacat tacaaggtga atgtccccgt tcagaaatcg tatgttgacg gtggacaaca
      361 gaaaaccgtt tcagggtata ttgttcagat ggaactggac aggaacttcc ggatacttac
      421 cctgaccgaa cgtaagccgt ctgccgacgc ggtctgaaaa ttctaatccc agctgagaat
      481 cactttccca cactgcccgc tatccatcac ttcgaaccct ttctggtatt catcgatgga
      541 aaaacgatgg gtgataacgg gggtcaggtc aattccggaa ataagcatct gctccatctg
      601 gtaccaggtt tcccacatct cacggccgta aatccctttc agcgtaaggg ctttaaagat
      661 gatctcgttc caatcgaccg tagtgttgtt gggtaaaata cccagtaacg atatttttga
      721 accgttgtac atgttgtcga tcatttcccg gaatgcagcg ggggagccgg acatttccaa
      781 ccctacgtca aagccgcgca ttcccagttt ttcaaccgct tgccggatgc tctctccttt
      841 cgaagggtta acggttagcg tagctcccat tttccgggct atctccagcc tgtaatcact
      901 caggtcgctg accacaacgt accgggcacc tgcaaagcgg catatggcgg ttgccatgct
      961 cccgataagc ccggcaccgg tgatgagcac atcttctccg atgagcggga acgataaggc
     1021 agtatgcgtg gcatttccga aaggatccat aatggacgcg atctcatcgg gaatacgggg
     1081 gtccagcttt acgacgtttt ctgccgggag cgacaagtat tcggcaaagg ctccgtcgcg
     1141 gttcacaccc accccgaggg tgttttcaca aacgtgtagc ttcccgcggc ggcagttgcg
     1201 gcagtgtccg caagcgatat gtccttcccc ggtaacccgg tcgccggttt tcaggttttt
     1261 tactccgctt cccatgtcca caatttcgcc cacgtattcg tgtccgatgg tcatgggggt
     1321 tttgatggtc ttttgcgccc aatcgtccca tttataaatg tgcagatcgg ttccgcagat
     1381 agaggtttta ataattttga tgagaacatc gttcactccg atctcgggaa cgggtatctc
     1441 ttgcatccat attccctttt cgggacgcag tttaactaag gctttcataa tgagtgtggg
     1501 ttatacgttg tgggttattt aataacggca agtttttttc cgactttaat aaatgcggca
     1561 atggctttgt cgaggtgttc acgtttgtgt ccggcggata gctgaacacg gatacgtgcc
     1621 tcccctttcg ggacgacggg ataatagaat cccgtaacgt agatgccttc atcgagcagg
     1681 gctgatgcaa attcctgtga cagcttggcg tcgtagagca ttacggcaca aatagccgac
     1741 tgggtaggtt tgatgtcgaa attggcttct ttcattttgg ctacgaaata atcgacattc
     1801 tcgtgcagct tgtcctgcaa ctcattcgat tcctgaagca attcaaacgc cttgattccg
     1861 gcagctgcca ccaacggagg aatggagttg gagaaaaggt aggggcgtga acgctgacga
     1921 agcatgtcga tgatctcctt gcgtccggta gtaaaaccgc ctatggcacc accaaatgct
     1981 tttccgagcg ttccggtaat gatttccgcc ttccccaggc agttgtattg ttcagtaacg
     2041 ccgcgtccgg tttttccgac gactcctgcc gaatggcttt catcgatcat caccagggca
     2101 tcgtacttct cggccaattc catgatttta tcgatgggag tcacatttcc atccatggag
     2161 aacacaccgt ccgttgcgat gatgcgaaag cgttgcgctt gtgcttcctg cagtttggct
     2221 tccaaatcgg ccatgtcggc gttggcgtag cgataacgtt tggctttgca caggcgcact
     2281 ccgtcgatga tggaggcgtg gttgagtgcg tctgaaacaa tagcatcttc ctcggtaaac
     2341 aggggttcga acaacccgcc gttggcgtcg aagcaggctg cgtatagaat ggtatcttcc
     2401 gttttgaaaa atttgctgat cgtagcttcc agctctttgt gaaggtcttg cgtcccgcaa
     2461 atgaaacgta cggatgacat tccgtacccg cgctcatcga gggctttttt ggctgcttcg
     2521 atcaggtggg cattgtctga caatccgagg tagttgttgg cacaaaagtt caatacttcc
     2581 tgtcccgatt ttactttgat ctctgccttt tgaggcgtaa cgataatccg ttcatttttg
     2641 taaagtcctg cttcttggat agcggctaat tcgctttgca ggtgttgttg cattttttcg
     2701 tacatagtgt tgttaagtta taagttgtga gagcctgggc ttttttactc ctcaatcagc
     2761 gattgcggca agcttttctt tgctttaact cccaaatctt ttagtttctg agtctgtaag
     2821 accaaattgt cgtttccggt agaaagctgt ttgtaggcat cgttgtacgc gttctgagcc
     2881 tggtccaggt ttttcccgat tttttcgagg ttattgataa accccacaaa cttatcgtag
     2941 agttttgccc cgcgttcggc aatctctatc gcatttcggt tctgatattc acgcttccag
     3001 aggtcaacga tgagctttaa cgctgcaatc aggtttgtag ggctgatgag caggattttt
     3061 ttgtcgtatg cgtattgcca gatatc
//
LOCUS       DAOSDR010000374         3084 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrimonas sp. isolate AMR_MDS_1826
            NODE_11222_length_3084_cov_3.263453, whole genome shotgun sequence.
ACCESSION   DAOSDR010000374 DAOSDR010000000
VERSION     DAOSDR010000374.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863355
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrimonas sp. (wastewater metagenome)
  ORGANISM  Petrimonas sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Dysgonomonadaceae; Petrimonas.
REFERENCE   1  (bases 1 to 3084)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3084)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:10:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,720
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 35
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3084
                     /organism="Petrimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11222_length_3084_cov_3.263453"
                     /isolate="AMR_MDS_1826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2023866"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..188)
                     /locus_tag="PLI30_12085"
     CDS             complement(<1..188)
                     /locus_tag="PLI30_12085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005844824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination NusG
                     family protein"
                     /protein_id="HOI80329.1"
                     /translation="MSENSKRWYVLRAIGGKEKKVKEYIENEIAKGDLQGFVTQVLIP
                     TEKVYQIRNGKKVSKERS"
     gene            complement(204..395)
                     /gene="secE"
                     /locus_tag="PLI30_12090"
     CDS             complement(204..395)
                     /gene="secE"
                     /locus_tag="PLI30_12090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013612173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HOI80330.1"
                     /translation="MKLKVYIEEAYDELVHKVTWPTWKELQSSALVVMVASFIIALVI
                     FVMDISFENIMGFIYGLFY"
     gene            complement(526..1713)
                     /gene="tuf"
                     /locus_tag="PLI30_12095"
     CDS             complement(526..1713)
                     /gene="tuf"
                     /locus_tag="PLI30_12095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005844822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HOI80331.1"
                     /translation="MAKEHFKRDKDHVNIGTIGHVDHGKTTLTAAITQVLAKKGLSEV
                     KSFDSIDNAPEEKERGITINTSHVEYQTATRHYAHVDCPGHADYVKNMVTGAAQMDGA
                     ILVVAATDGPMPQTREHILLARQVNVPRIVVFMNKVDMVDDPEILELVEMEVRDLLSF
                     YKFDGDNTPVIQGSALGALNGEPQWEDKVMELMDAVDAWIPLPPRDKDKPFLMPVEDV
                     FSITGRGTVATGRIETGVVHTGDELQIIGLGATGRKTVCTGVEMFRKILDDGQAGDNV
                     GLLLRGVDKKEIKRGQILAKPGSITPHTKFKAEIYVLKKEEGGRHTPFHNKYRPQFYL
                     RTLDVTGEIHLPEGREMVMPGDNVSIEVELIYPVALNLGLRFAIREGGRTVGAGQVTE
                     IIE"
     gene            complement(1753..1824)
                     /locus_tag="PLI30_12100"
     tRNA            complement(1753..1824)
                     /locus_tag="PLI30_12100"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1790..1792),aa:Thr,seq:ggt)
     gene            complement(1836..1908)
                     /locus_tag="PLI30_12105"
     tRNA            complement(1836..1908)
                     /locus_tag="PLI30_12105"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1873..1875),aa:Gly,seq:tcc)
     gene            complement(2177..2479)
                     /gene="raiA"
                     /locus_tag="PLI30_12110"
     CDS             complement(2177..2479)
                     /gene="raiA"
                     /locus_tag="PLI30_12110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008152455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_component: GO:0022626 - cytosolic ribosome [Evidence
                     IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HOI80332.1"
                     /translation="MNIKVNSVHFNADQKLIDFATKKVNKLDVFFDGILGAEVTFKVL
                     KPEAANNKIAEIKLSIPATDNLFAKKQADTFEEATDLAIDALRRQLDKFKDKIRAK"
     gene            complement(2493..>3084)
                     /locus_tag="PLI30_12115"
     CDS             complement(2493..>3084)
                     /locus_tag="PLI30_12115"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HOI80333.1"
                     /translation="PLPKVRKKLPGFVGEKNLHHLLDDGFFSKDFSGIRDKLIVEMLY
                     GTGVRLSELLNLNENHIDLKSYQIKVTGKRNKERIIPFPRTLKPLIEEYRLLKKEQFG
                     FEGGVLLLTDKGEPAYEKLIYRVVKTNLSKVTSLEKRSPHVLRHTYATHLLNKGADLN
                     AVKELLGHSNLSATEIYTHTTFEKLHRIYEQAHPRG"
BASE COUNT          802 a          738 c          630 g          914 t
ORIGIN      
        1 aagcttctct ctttgctgac ttttttcccg tttctgattt gatagacctt ttcggttgga
       61 attaaaacct gtgtaacaaa accctgaaga tcacctttgg cgatttcgtt ctcaatgtat
      121 tccttcacct tcttctcttt tccgcctatc gctcgaagga cataccacct tttactattt
      181 tcgctcatag tgactccagc caattaataa aataagccat agataaatcc cattatgttc
      241 tcaaaagaga tgtccataac gaaaattacg agtgctatga taaaggaagc aaccatcaca
      301 actaatgcac tgctttgcag ttctttccaa gtaggccatg taaccttatg aaccagttca
      361 tcataagcct cttcgatata tactttgagt ttcatcccaa taattgattt tggccggatg
      421 gcaagattcg aacttactgt ttttcaacaa ctcccaactg agtaacaccc gaaaatagtc
      481 ccggataaca tccgggacta ttttgtgtat tgtttaatct ttggattatt caatgatttc
      541 agttacctga cctgcaccta cggtacgtcc accttcgcgg atagcaaaac gaagaccgag
      601 attgagtgct acaggataga tcagttccac ttcgattgaa acgttatcgc caggcataac
      661 catttcacgt ccttcgggga ggtgaatttc accggttacg tcaagggtac gcaggtagaa
      721 ctgcggacga tatttgttgt ggaacggcgt gtgacggcca ccttcttctt ttttcagaac
      781 gtagatctca gctttaaatt tggtatgcgg agtaatggag cccggttttg ccaaaatctg
      841 accacgtttg atctcttttt tgtcaacacc acggagcaac agtcctacgt tatccccagc
      901 ctggccatcg tccagaattt tgcggaacat ctcaacaccg gtacagacgg ttttgcgtcc
      961 ggtagcgcca agaccgataa tctggagctc atcacctgtg tgaacaacac cggtttcgat
     1021 acgaccagtt gcaaccgtac cacgaccagt aatcgagaat acgtcttcaa caggcatcag
     1081 gaacggtttg tctttatcac gcggaggcag cggaatccaa gcatcaactg catccatcag
     1141 ttccataact ttgtcttccc actgcggttc gccattgagt gcaccaagtg ctgacccctg
     1201 gataaccgga gtgttgtcgc cgtcaaattt gtagaagctc aacaggtcgc gtacttccat
     1261 ttcaaccagc tccaggattt ccgggtcgtc aaccatgtcc actttgttca tgaaaacaac
     1321 gatacgcgga acgtttacct ggcgggccaa cagaatgtgc tcgcgggttt gcggcatcgg
     1381 accatcagta gcagctacaa caaggatagc accgtccatc tgggcagcac cagtaaccat
     1441 gtttttcaca tagtcggcgt ggccgggaca gtcaacgtgt gcatagtgtc gagttgcggt
     1501 ctggtattct acgtgtgagg tgttaatggt aatacccctt tctttttctt caggagcatt
     1561 gtcaatagag tcgaatgatt taacttcaga cagacctttt ttagccaaaa cctgcgtgat
     1621 ggcggcggtc agggtagttt taccatggtc aacgtggcca atcgtcccaa tgttgacatg
     1681 gtctttgtcc cttttaaaat gttctttagc cataactcga aaaattttat ttatttattt
     1741 taaaatcatt tgagccggag acgagaattg aactcgtgac ctcttcctta ccaaggaagc
     1801 gctctacccc tgagctactc tggctttaaa atttgagcgg gaaacgggac tcaaacccgc
     1861 gaccctcagc ttggaaggct gatgctctac cgactgagct actcccgcat tttgcctttt
     1921 taaaaggccg tgcaaatgta taaaaaaatt atccaaagaa ccgccctgcc tgaaatattt
     1981 tcgagaatgt ttttctgagt atcgtctttt tcaaaacttt cttcagctta aaaactccct
     2041 cgtcatctat ttcgggaacc gtttaaaact gcgttgtttc gcttcaaaca ggaaatcaca
     2101 ggacgccccg aaaagggtct gcaaatgtaa aaatattttt atacaattcg cacctggttc
     2161 aaacaaattt gattttttat tttgcccgaa ttttgtcttt aaatttatct aactgacggc
     2221 gaagagcatc tattgccaaa tcggtagcct cttcaaaggt atcagcctgc ttcttggcaa
     2281 ataaattatc agtggctgga atagagagtt tgatctcggc gatcttgttg ttggccgctt
     2341 cgggcttcaa cactttaaac gttacctcag cgccgaggat accgtcaaaa aaaacatcca
     2401 gcttgtttac ctttttggta gcaaaatcga tcagcttctg atcggcgttg aaatgcactg
     2461 aatttacctt gatattcata atccatcagt ttttaacccc tggggtgggc ttgttcataa
     2521 atacgatgta atttttcaaa tgtagtatgg gtgtaaatct cggttgccga caggttggag
     2581 tgccccagca attccttcac ggcattgaga tcggcacctt tattgagcag atgagtcgca
     2641 taagtgtgcc ggagcacgtg cggactcctc ttttcgagcg aggttacttt ggataaattc
     2701 gtcttcacaa cccggtaaat taacttttcg tatgccggtt cgcctttgtc ggtcagcagc
     2761 aaaacgcctc cttcaaaacc gaactgttct tttttcagca aacgatattc ttctattaaa
     2821 ggtttcagtg tgcgcggaaa aggaatgatg cgctctttgt ttcttttacc ggttactttg
     2881 atctgataac ttttcaggtc gatatggttc tcgttgagat tcagcaattc gctcaaccta
     2941 acccctgtcc cgtaaagcat ctccacaata agcttgtccc tgatacccga aaaatctttc
     3001 gaaaaaaaac cgtcatccaa caggtgatgc agattcttct cccccacaaa gccaggtaat
     3061 tttttcctca ctttgggcaa agga
//
LOCUS       DAOSDR010000375         3066 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrimonas sp. isolate AMR_MDS_1826
            NODE_11296_length_3066_cov_4.256725, whole genome shotgun sequence.
ACCESSION   DAOSDR010000375 DAOSDR010000000
VERSION     DAOSDR010000375.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863355
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrimonas sp. (wastewater metagenome)
  ORGANISM  Petrimonas sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Dysgonomonadaceae; Petrimonas.
REFERENCE   1  (bases 1 to 3066)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3066)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:10:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,720
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 35
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3066
                     /organism="Petrimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11296_length_3066_cov_4.256725"
                     /isolate="AMR_MDS_1826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2023866"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..765)
                     /locus_tag="PLI30_12120"
     CDS             complement(<1..765)
                     /locus_tag="PLI30_12120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019539937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOI80334.1"
                     /translation="MNNKLQKDNGIRVTKTSLWRRLGGALFDLDRWYEIWVTITHNKS
                     RSFLTAFGVFWGMFMLVLMVGAGNALSKGISSQVEGFAGNSCFFWTQNTSEAYKGFRK
                     GRRWDMLNSDVEIIRSKVPELQHLSPMLFGGSNDKNVVRGEKGGSYQVKGCYPEYDLI
                     EKSKIISGRFINDIDIAENRKVCIIGERVYEVLFQTGEDAIGKLVKVNGIYFQVIGVA
                     RRMANASIGGSTDESVILPFSTMQKAFNQGNIIHFLG"
     gene            complement(791..2053)
                     /locus_tag="PLI30_12125"
     CDS             complement(791..2053)
                     /locus_tag="PLI30_12125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019539936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOI80335.1"
                     /translation="MNDHLQEILQTLRKNKLRTALTGLSVSWGIFILIVLLGAGNGLK
                     NGVMENFSDRAVNRITLWSGTTSMAHNGLKSGRNLQFANKEVDLIRTDVNESRLIAAR
                     VDRTQTISFGPEYGSYNVRGVMPDYREIERLVIKPGDGRFINGLDVKETNKIIILDNK
                     IAETLFKGEKALGNYVKVGELMFKVVGINTKKERWGTPTAYIPFSTAQAIFNPDKKFN
                     QITFTVDGLETKEENDRFDKSLRSLLGRSLNFNPEDRQALWVNNSQAEYIETQKIFGG
                     ITLFVSIIGILTLIAGIVGVSNIMLVSVKERTREIGIRKAIGASPLSILKTIILESLI
                     ITAIFGYIGMILGVGLTELINFVMEQSANGQPVTDEVQMSVFKNPTVNMGYAIFATVV
                     LVIAGIIAGYLPARKAVRIKPIEAMRQE"
     gene            complement(2059..2766)
                     /locus_tag="PLI30_12130"
     CDS             complement(2059..2766)
                     /locus_tag="PLI30_12130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019539935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOI80336.1"
                     /translation="MIKLKKINKSYHTEAISLHALKDIDLEIESGEYVSIMGASGSGK
                     STLLNLMGILDSYDSGEYRLNGTLIRNLTETQAAKYRNELIGFVFQSFNLINFKNALE
                     NVALPLYYKNVSRKKRNIIAMEHLDRMGLKNWAHHLPGELSGGQKQRVAIARAMISNP
                     KVILADEPTGALDSQTTVEVMDVLSDLNRQGITTIIVTHEQSVADVTNRIIRLKDGEI
                     ESEIRKDGAHRFYTLNS"
BASE COUNT          745 a          714 c          678 g          929 t
ORIGIN      
        1 cccgagaaaa tggatgatgt tgccttgatt aaatgctttt tgcatggtgc tgaaaggcag
       61 gatgaccgat tcatcggttg agccaccaat gcttgcattg gccattctac gggccacgcc
      121 gataacctga aaataaatac cgttcacctt taccagtttt ccaatggcgt cttctccggt
      181 ttgaaacaac acttcgtaaa cacgttcacc gataatgcat accttgcggt tttcggcaat
      241 atctatatcg ttgataaatc ttccgctgat tattttactt ttttcaatca ggtcgtattc
      301 ggggtagcac ccttttacct ggtacgatcc gcctttttcc ccacgaacca cattcttatc
      361 gttagaaccg ccaaagagca tgggcgagag gtgttgcaac tcaggtactt tgctgcggat
      421 gatttccaca tcgctattca acatgtccca ccgacgtccc ttgcggaacc ccttgtacgc
      481 ttccgatgtg ttttgtgtcc agaaaaagca ggagttgcct gcaaaccctt ctacctggga
      541 ggagatgcct ttactcaacg cgtttcccgc tccaaccatc aatacaagca taaacatccc
      601 ccagaatacc ccgaatgccg tgaggaaact gcgcgatttg ttgtgggtaa tggttaccca
      661 tatctcgtac cagcggtcga ggtcgaataa agccccgccc aatcgcctcc aaagggaagt
      721 tttggttaca cgaataccgt tatctttttg caatttgtta ttcatttttt tctttgacga
      781 gtttcagcaa tcattcctgg cgcatggctt caatcggttt tattctgacc gcttttcggg
      841 caggcaggta tccggcaatg atgcccgcga taacaaggac aacggtagcg aaaatggcat
      901 atcccatgtt gactgtgggg tttttaaaaa ccgacatctg gacttcgtcg gtaacgggtt
      961 gtccgttggc ggactgttcc attacgaagt ttatcagctc ggttaacccg acccccagga
     1021 tcatcccgat atatccgaaa atagccgtaa tgatcagcga ttccagaatg atggttttca
     1081 gtatagacaa cggggatgcg cctatagctt tccggattcc gatctccctt gtccgctctt
     1141 tgacggagac cagcatgata ttgcttactc cgacaatgcc tgcaattagg gtgaggatcc
     1201 cgatgatgct gacaaacaaa gtgattcctc caaaaatctt ttgcgtttca atatattcgg
     1261 cctgtgagtt gtttacccac agagcctgcc tgtcctccgg attgaaatta aggcttcttc
     1321 ccagcaagct acgtagggat ttatcgaaac ggtcattttc ttctttcgtt tccagtccgt
     1381 ccacggtgaa cgtaatctga ttgaacttct tgtccggatt aaaaatagcc tgtgccgttg
     1441 agaacggaat gtaggcggtt ggcgttcccc aacgttcttt tttggtgttt atgccaacaa
     1501 ctttaaacat cagttcccct accttcacgt agtttcccaa cgccttttct cctttgaaga
     1561 gcgtttcggc aattttattg tctaggataa ttattttgtt ggtctctttt acatccaggc
     1621 cgttgataaa acgcccatcg ccgggtttaa tcaccagcct ttctatttcc cggtaatcgg
     1681 gcattacgcc gcgtacgttg tatgagccat attccggacc gaaggagatg gtttgtgtcc
     1741 tgtctacacg tgcagcaata agacggcttt cgtttacatc ggttctgata aggtctactt
     1801 ctttgtttgc aaactgcagg tttcgccccg atttaagccc gttgtgtgcc atggatgttg
     1861 tccctgacca gagtgttatg cggtttacag ccctgtcgct gaagttttcc atgaccccgt
     1921 ttttcaatcc gttccctgca cccaacaaaa caatgaggat gaagattccc caagaaaccg
     1981 aaagtccggt gagcgccgta cgtagcttat ttttacgcag ggtctgaagt atttcctgaa
     2041 ggtggtcgtt catatttgtt acgagttgag agtgtaaaaa cggtgcgcgc cgtctttacg
     2101 aatttccgat tcaatttccc cgtcttttag ccggataatc cgattggtga catcggcgac
     2161 tgactgttca tgagtgacaa tgatggtggt gatgccctgt ctgtttaaat cggaaaggac
     2221 atccattact tcaacggtgg tttggctatc gagagcgcca gtaggttcgt cggcaaggat
     2281 cacttttgga ttcgatatca tagcgcgtgc aatggcaacc cgttgtttct gcccacccga
     2341 taactcgccg ggcaagtggt gtgcccagtt ttttaacccc atcctatcga ggtgttccat
     2401 tgcgataatg tttcgctttt tccggctgac atttttgtag tagagaggga gagccacgtt
     2461 ttccaatgcg tttttgaagt tgatcaggtt gaacgattga aacacgaatc ctatcagctc
     2521 gttgcggtat ttggctgctt gtgtctcggt caggtttctg atcagggtac cgttaaggcg
     2581 atattcgccc gaatcgtaac tgtccaggat tcccattaaa tttaataatg tggatttccc
     2641 ggaaccggag gctcccataa ttgagacgta ttcaccggac tcaatctcca gatcgatatc
     2701 tttaagggca tgcagagaaa tggcttcggt gtggtacgat ttgtttattt ttttgagttt
     2761 aatcatgaag agaatctaat taaattgaag tattctaatt gaattcggat acaactattc
     2821 tatcaatgga acaaaattaa tgctttgaat aatactatgc aatacataat actagtaaag
     2881 ttataaaaat tatgaataat taactatttt aacgtataac tcatgcctcc cgtcagccag
     2941 aatcccggtt gcggaatatt cccaaaatcc acatgcgtgg tgttgaaaat gttattggca
     3001 ttaacaaaaa tgtttatgtt gcgtaaagcg taattggctt tgacatccac taatccgaac
     3061 ggttgg
//
LOCUS       DAOSDR010000376         3062 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrimonas sp. isolate AMR_MDS_1826
            NODE_11315_length_3062_cov_4.494845, whole genome shotgun sequence.
ACCESSION   DAOSDR010000376 DAOSDR010000000
VERSION     DAOSDR010000376.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863355
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrimonas sp. (wastewater metagenome)
  ORGANISM  Petrimonas sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Dysgonomonadaceae; Petrimonas.
REFERENCE   1  (bases 1 to 3062)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3062)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:10:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,720
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 35
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3062
                     /organism="Petrimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11315_length_3062_cov_4.494845"
                     /isolate="AMR_MDS_1826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2023866"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(155..1609)
                     /locus_tag="PLI30_12135"
     CDS             complement(155..1609)
                     /locus_tag="PLI30_12135"
                     /EC_number="1.11.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018360855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004096 - catalase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catalase"
                     /protein_id="HOI80337.1"
                     /translation="MTTVAGAPVGNNQDAMTAGPRGPMMLQDVWFLEKLAHFDREVIP
                     ERRMHAKGSGAFGTFTVTHDITPYTKAKIFSEIGKKTEMFVRFSTVAGERGAADAERD
                     IRGFAMKFYTEEGNWDLVGNNTPVFFFRDPLKFPDLNHAVKRDPYTNLRSPNNNWDFW
                     SSLPEALHQVTITMSDRGIPRSYRHMHGFGSHTFSLINADNQRFWVKFHFVTQQGIEN
                     LTDQEAIELVGNDRESHQRDLFEAIGNGNYPKWKMFIQVMTEEQAESMPYNPFDLTKV
                     WYKGDFPLIPVGEFELNRNPENYFQDVEQAAFNPANIVPGIGFSPDRMLQGRLFSYGD
                     AQRYRLGVNHHQIPVNMPRGATHTYNSFHRDGQMRIDGNHGAALHYEPNSYNIWREQP
                     EHDEPTQKVSGDIKRWNFREDDDNYYEQPGKLFRLMTPDAQQRLFENTARNMNGVEEH
                     IKIRHIGNCFKADPNYGRGVADACGIPYEKAGIN"
     gene            complement(1722..2189)
                     /locus_tag="PLI30_12140"
     CDS             complement(1722..2189)
                     /locus_tag="PLI30_12140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004380639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HOI80338.1"
                     /translation="MKTTEFTGLRNVENVTKGLQQLLADLQVYYTNLRGFHWNIKGKD
                     FYLLHEKFEEMYNDAAAKVDEVAERLLMLGETPAHTFTKYLKTANVKETGVVTGTDEA
                     IKNILDTLKLLIASERELLALASEIDDEVTVALLSDYISGQEKEVWMLTSFLS"
     gene            complement(2195..2647)
                     /locus_tag="PLI30_12145"
     CDS             complement(2195..2647)
                     /locus_tag="PLI30_12145"
                     /inference="COORDINATES: protein motif:HMM:NF013629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HOI80339.1"
                     /translation="MHVDIKHIQELIKEKDLKVTPQRIGVLEAIYTLRNHPTAEQIID
                     FIHDKYPSIAIGTVYKTLDTFVKYGVINKVLTEGEVMRYDGVLKHHHHLYNEGNEEIQ
                     DYINEELDEMLKTYFEKNAIEGYHISSITLHISGKSINKKSINKNNKK"
     gene            complement(2759..>3062)
                     /locus_tag="PLI30_12150"
     CDS             complement(2759..>3062)
                     /locus_tag="PLI30_12150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004331929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HOI80340.1"
                     /translation="GFIIIGGVDNVARFLLQKVLADIHPLITIFGVLIGIPMFGFWGV
                     IFGPLLLSLFVLFFNMYRHEYIPGSTAQPRVTTRMKSRKFSIPRYKKTKENNLKTS"
BASE COUNT          717 a          684 c          708 g          953 t
ORIGIN      
        1 tggtttggag cctaagggaa ggaatgcttc acagacttta cagttccgta tttcgcgtaa
       61 caattcattc atttccttga tcagttccgg aaaatcacaa cggtatccgg accggagtta
      121 tttgatgaca aattggcgga taatggaaaa ccggtcagtt tattcctgcc ttctcgtacg
      181 gaattccgca ggcatcggca acccctcttc cataattcgg atcggcttta aaacaatttc
      241 ctatgtggcg gattttgata tgttcttcca caccgttcat attccgcgcc gtattttcga
      301 acaaccgttg ctgtgcatcg ggagtcatca gccggaatag tttgccgggt tgctcatagt
      361 agttgtcatc gtcttcacgg aagttccatc gtttgatatc accgcttact ttctgggtcg
      421 gctcatcgtg ttcgggttgt tcacgccaga tgttgtagct gttgggttcg taatgcaatg
      481 ctgcgccgtg gttcccgtca atacgcatct gtccgtcgcg gtggaacgag ttgtaggtgt
      541 gcgtagcccc ccttggcatg tttacaggaa tctggtggtg gttcactccc agccggtagc
      601 gttgtgcatc tccgtatgag aataaacgcc cctgtaacat cctgtcggga gaaaagccta
      661 ttcccggaac aatgttggcc ggattgaagg cggcttgttc gacgtcttga aaatagtttt
      721 cggggtttcg gttcagttca aactctccca ccggaattag cgggaagtcg cctttatacc
      781 aaacctttgt caggtcgaac gggttgtaag gcatgctctc ggcttgttct tccgtcatta
      841 cctggatgaa catcttccat ttcggataat tgccgttgcc gatagcttca aacagatccc
      901 tctgatgact ttcgcggtcg ttcccaacca gttcgatcgc ttcctggtcg gtcaagtttt
      961 cgatgccctg ttgggttaca aaatggaact ttacccagaa acgctggttg tccgcgttga
     1021 tcaaactgaa cgtatggctt ccgaagccgt gcatgtgccg ataggagcgg ggtatccccc
     1081 tgtcgctcat cgtgatggta acctgatgca atgcctcggg aaggctactc cagaagtccc
     1141 agttgttgtt gggactgcgc aggttagtgt aaggatcacg ttttaccgcg tggttcaaat
     1201 cagggaattt cagggggtcg cggaaaaaga agaccggtgt gttgttgccg accagatccc
     1261 agttgccttc ttcggtataa aacttcatgg caaagccacg gatgtccctt tcggcatcgg
     1321 ctgccccacg ctcaccggca acggtcgaaa aacggacaaa catttctgtc ttcttgccaa
     1381 tctcggagaa gattttcgcc ttggtgtaag gtgtaatgtc gtgggtaacg gtgaaggtgc
     1441 cgaaggcacc cgacccttta gcatgcatac gtcgctccgg aattacctcg cgatcgaaat
     1501 gagcaagttt ctccaaaaac cacacgtctt gcagcatcat aggcccgcgt ggcccagcgg
     1561 tcattgcgtc ctggttgttg ccaaccggag ctccagcaac cgtggtcatc ggtttttgat
     1621 tttctttgtc tctttttttc ttcaattctt ctgcgttcat accatacaat gtttgttgtt
     1681 aacgattaat gtctaaaaaa aaatttttta gcagatgcag attaggacaa aaatgaagta
     1741 agcatccaaa cctctttttc ctgtccgctg atgtagtcgc tcagaagcgc tacggttact
     1801 tcatcatcaa tctctgaagc caaggcaagc aattctcttt cggaagcgat gagcaatttg
     1861 agcgtgtcga gaatgttttt gatggcttca tcagtaccgg taactacgcc ggtttctttt
     1921 acgttggcag ttttcaagta ctttgtgaag gtgtgggccg gagtttcacc aagcataagg
     1981 agtctttcgg caacttcatc tactttagcg gcagcatcgt tgtacatctc ttcgaatttt
     2041 tcgtggagaa ggtaaaaatc cttgcctttt atgttccagt ggaatccccg taaattggtg
     2101 taataaactt gtaggtctgc cagtaattgc tgtaacccct tggttacatt ttctacattt
     2161 cttaatcctg taaattcagt tgttttcatt gttttcattt tttattgttt ttgtttattg
     2221 attttttgtt tattgatttt ccactgatat gaagggtgat cgaacttata tgataacctt
     2281 cgatagcatt tttttcgaaa taagttttca gcatttcatc cagttcttcg tttatgtaat
     2341 cctgaatttc ctcgttgcct tcattgtaca ggtggtgatg gtgtttcaag acgccatcgt
     2401 aacgcataac ttctccttcg gttaagacct tattgataac cccgtatttg acaaaagtgt
     2461 cgagtgtttt gtaaaccgtt ccaatagcta tgctggggta tttgtcatga ataaaatcaa
     2521 tgatctgttc agccgttgga tgattacgta acgtgtaaat cgcttcgagt accccgattc
     2581 gttggggagt tactttcaga tctttctctt ttatgagctc ctgaatgtgt ttaatatcta
     2641 catgcatctt aatcctaata aagaattatt cttacataaa acaaattctc ggaggaattg
     2701 ttcacgtaag ataatttttt ttgcctgttt tggattagaa catccgaaat cagtacggtt
     2761 acgacgtttt aagattgttt tcttttgtct ttttgtatcg gggtatgctg aatttccggg
     2821 acttcatccg ggtggtgaca cgtggttgtg ccgttgagcc cggaatgtac tcgtgccggt
     2881 acatattgaa gaagagcaca aacagggaga gcagcagggg gccgaaaata acgccccaga
     2941 acccgaacat gggaattccg atcaatactc cgaaaatggt tatcaacgga tgaatatcgg
     3001 ccaatacctt ttgaagcaga aaacgcgcaa cattgtcaac accgccaata ataatgaatc
     3061 cg
//
LOCUS       DAOSDR010000377         3032 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrimonas sp. isolate AMR_MDS_1826
            NODE_11442_length_3032_cov_3.058784, whole genome shotgun sequence.
ACCESSION   DAOSDR010000377 DAOSDR010000000
VERSION     DAOSDR010000377.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863355
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrimonas sp. (wastewater metagenome)
  ORGANISM  Petrimonas sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Dysgonomonadaceae; Petrimonas.
REFERENCE   1  (bases 1 to 3032)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3032)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:10:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,720
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 35
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3032
                     /organism="Petrimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11442_length_3032_cov_3.058784"
                     /isolate="AMR_MDS_1826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2023866"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..424)
                     /locus_tag="PLI30_12155"
     CDS             complement(<1..424)
                     /locus_tag="PLI30_12155"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019540190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HOI80341.1"
                     /translation="MSYTETNTNVITFKVGMLEVRIFPNREEMGKEAAVDVSDTIRQL
                     LNEKDEINMIFAAAPSQSDFMKELTADKRIRWEKINAFHMDEYIGLEKDAPQGFGNFL
                     RERIFEKVPFKSVHYINGLAEDPSAECERYAGLLTRYPV"
     gene            complement(405..1592)
                     /gene="nagA"
                     /locus_tag="PLI30_12160"
     CDS             complement(405..1592)
                     /gene="nagA"
                     /locus_tag="PLI30_12160"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019540191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008448 - N-acetylglucosamine-6-phosphate
                     deacetylase activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HOI80342.1"
                     /translation="MNRLIIKNGKVIFPEKIEENVTVVCENGKITDILNNEEYIHGND
                     DVVIDATNKYIAPGFIDIHTHGGGGHDFMDGTVEAYLGAAETHARYGTTALLPTTLTS
                     TFDELINTFAVYKEAARLNTKGAKLLGLHLEGPYFAYNQRGAQDPKYLRNPEPEEYNK
                     ILSESDDIVRWSLAPELPGAFDFGKVLTSKNILTSIAHSDAIYEEVLDAFNAGFTHVT
                     HLYSAMSTVTRRNAFRYAGVLEAAYLIDDMTVEIIADGVHLPKSLLQFVYKFKGSEKT
                     ALCTDSMRGAGMPDGESILGSLEKGQRVIIEDGVAKLPDRTAFAGSVATTDRLVRTMV
                     NVAEVPLIEAVKMMTLTPARIMKIDQQKGSVRKGKDADLVVFDNNINVSYTILEGNVI
                     YGN"
     gene            complement(1626..3002)
                     /locus_tag="PLI30_12165"
     CDS             complement(1626..3002)
                     /locus_tag="PLI30_12165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019540192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOI80343.1"
                     /translation="MSTTPPMNQARNKSTTIKSMIILALLFFIFGLVSWVNTILVPYF
                     QLTLQLSNFQSSFVTFAFYIAYLVMAIPSSFLLNKVGYKRGMMFGLWCMAIGALLFVP
                     AAYWRLYQLFLLGLFLLGVGLAILQSAANPYVTIVGPIESAAKRMSIVGTGNKLAGVI
                     ANLIFAGVVIRESDKVLMREIESGLYTGATLETALDTLIKGVMTPYLILAIALFIFGI
                     VVRYSALPELDPSVVNKRSAEDESSQKSIFQYPALILGVIAMFFHIGTQMIALATSIQ
                     YAGTMGESLAGPAKNIPSYTMLLTFLGYFIGIILIPRYLKQRNALLICALLNLSLSVL
                     IISTSGTVNFLGITSDISIWYLVMMGLPNALLYAGIWPLAINGLGKHTNLGSAFLVMA
                     LCGSAFMPMIYHSLVELNSSLSPFAAMKYAYWILIPSFAYIVWYAAYGYRIRSWKKSE
                     VVKNTVVS"
BASE COUNT          899 a          697 c          582 g          854 t
ORIGIN      
        1 ctaccggata tcgtgtcagc aagccggcat atctctcaca ttcagcagaa ggatcttcag
       61 caagcccatt tatgtaatgt acacttttaa aagggacctt ttcaaatatc ctttctctaa
      121 ggaaattacc gaacccttgg ggtgcatcct tctccaatcc aatatactca tccatatgaa
      181 aggcattgat cttctcccac cggatccgtt tatcagctgt taattccttc ataaaatcac
      241 tttgcgatgg agcagctgcg aaaatcatat tgatctcgtc tttttcatta agcaactgtc
      301 ttatagtgtc agatacatcg actgctgcct ctttacccat ttcctccctg ttagggaaaa
      361 ttctcacttc caacattcct actttgaatg tgattacgtt tgtgttagtt tccgtatatg
      421 acatttcctt ccaagattgt gtatgaaaca ttaatattat tatcaaaaac aactaaatcg
      481 gcatctttcc ctttacgaac agaccccttc tgctgatcta tcttcattat gcgagccgga
      541 gtcagcgtca tcattttcac agcttctatc aacggcactt ccgctacgtt taccattgtt
      601 cttaccagac gatctgtagt tgccacactt cccgcaaaag cggtacggtc gggcaacttg
      661 gctacgccat cttcgataat taccctttgt cctttttcca agctgcccag gatggattca
      721 ccgtccggca ttcccgctcc ccgcatagag tcggtacata gtgctgtctt ttcggatcct
      781 ttgaatttat atacgaactg cagaagagac ttgggcaggt gtactccatc agcaataatt
      841 tccaccgtca tatcatcaat caggtacgcg gcttccaaaa cacctgcata acggaatgca
      901 ttcctacggg taacagtaga catagccgaa tagagatggg tcacgtgggt gaaccctgcg
      961 ttaaaggcat ccaacacctc ctcgtaaatg gcatcgctat gtgctatgga agtgaggata
     1021 ttttttgaag tcagcacctt tccaaaatcg aatgcgccgg gcagttccgg tgccaggctc
     1081 caccgtacaa tatcgtctga ttcggataaa atcttattgt actcttccgg ttcagggttt
     1141 ctaaggtatt tgggatcctg cgctccccgt tgattgtatg caaaataagg tccttctaaa
     1201 tgcaagccca aaagttttgc accttttgtg ttcagtcttg cggcttcctt gtatacggca
     1261 aacgtattta tgagctcgtc aaacgtgctt gtcaacgttg taggcaataa tgcagtggtt
     1321 ccatacctgg cgtgcgtttc tgctgctccc agataagctt ccaccgttcc gtccataaaa
     1381 tcgtggccgc ctcctccatg cgtgtgaata tctataaacc cgggggcaat atacttattt
     1441 gttgcgtcta tcacaacatc atcattaccg tgtatgtact cttcattatt aagaatatcg
     1501 gttatttttc cgttttcaca caccacggtt acgttttctt caattttttc cggaaagata
     1561 acttttccgt tttttatgat aagtctattc atcatccttt gatgtttatg tgttaattaa
     1621 atgtattaag aaacaaccgt atttttgaca acctcgcttt ttttccatga tcttattcta
     1681 tatccgtaag cagcatacca aacaatataa gcaaaagagg gaataaggat ccaataagca
     1741 tatttcattg cagcaaaagg cgaaagagac gaattaagct ccacaagtga atgataaatc
     1801 atcggcataa atgcgcttcc acaaagcgcc attaccaaaa aagcagagcc gagattggta
     1861 tgcttcccta atccgttaat agcaagaggc cagattccgg cgtaaagcaa tgcattggga
     1921 agtcccatca ttacaaggta ccagatagaa atgtcggatg taataccgag aaaattcacc
     1981 gttcccgaag tgctaatgat taaaacagaa agcgataaat tcaataaagc acaaatcaag
     2041 agcgcgttac gttgcttcag gtatctgggg attaaaatga ttccgataaa atatcccaaa
     2101 aaagttaaca acatggtata ggaagggata tttttagctg gaccggcgag gctttcaccc
     2161 attgtgcctg catactgaat actggtagca agagcgatca tctgtgttcc tatatggaaa
     2221 aacatggcaa ttactcccaa tatcagtgca ggatactgaa agatggattt ctgtgaactt
     2281 tcatcttcag cagaacgttt atttactacc gagggatcta attccggcaa agccgaataa
     2341 cggacaacaa ttccaaaaat aaaaagcgcg attgctaaaa tcaggtatgg tgtcataact
     2401 cccttaatca gggtgtccaa agcagtctct aacgttgcac cggtgtataa accggattct
     2461 atttcacgca tcaacacctt gtcggattca cggataacaa ctccggcaaa aataaggtta
     2521 gcgattactc ctgccagttt attacctgtt ccaactatgc tcatcctttt ggcggcactc
     2581 tcaataggcc cgacaatggt tacgtaagga ttagcagcag actgcaaaat agccagcccc
     2641 acgcccagca ggaagagtcc caggagaaac aactgataaa gacgccaata ggcagcagga
     2701 acaaagagca gtgctccgat tgccatgcac cagagtccaa acatcattcc tcttttatat
     2761 cccaccttat tcagtaaaaa agaagaaggg atagccatca ccaaataagc aatataaaat
     2821 gcaaatgtaa caaaagagga ttggaaatta gatagctgaa gggtcagctg aaaataagga
     2881 accaggatag tgtttaccca ggaaaccaac ccgaaaataa aaaacaacaa tgccaggata
     2941 atcatcgact tgatggtggt acttttattc cgggcttgat tcattggagg tgtagtactc
     3001 atatttacag taaattttta acaataagaa gt
//
LOCUS       DAOSDR010000378         3022 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrimonas sp. isolate AMR_MDS_1826
            NODE_11477_length_3022_cov_4.387597, whole genome shotgun sequence.
ACCESSION   DAOSDR010000378 DAOSDR010000000
VERSION     DAOSDR010000378.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863355
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrimonas sp. (wastewater metagenome)
  ORGANISM  Petrimonas sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Dysgonomonadaceae; Petrimonas.
REFERENCE   1  (bases 1 to 3022)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3022)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:10:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,720
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 35
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3022
                     /organism="Petrimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11477_length_3022_cov_4.387597"
                     /isolate="AMR_MDS_1826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2023866"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..408
                     /locus_tag="PLI30_12170"
     CDS             <1..408
                     /locus_tag="PLI30_12170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase family protein"
                     /protein_id="HOI80344.1"
                     /translation="LVEVIRGQLTADSVFDSVSGIAKNIGKVPVTVNEAPGFLVNRLL
                     IPMINEGIGALAEGVASKEEIDEAMKLGAGHPIGPLALADLIGLDVCLAILEVLHHEF
                     GDDKYRPHPLLRKMVRANLLGRKIGTGFYDYQK"
     assembly_gap    416..425
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<426..1593)
                     /locus_tag="PLI30_12175"
     CDS             complement(<426..1593)
                     /locus_tag="PLI30_12175"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOI80345.1"
                     /translation="MPEEVTTQRKIFGFSSNVFYAGIVSFLTDTSTKMVYSVVPMFLM
                     SIGVSKTSLSLIEGVAESTASLLKAFSGYWSDKIGRNKPFMVIGYGLSSLILPLYATV
                     VTPLQMLLFRFAERVGKGIRSAPRDSLVAGSTEKNETGKSFGLHKAMDNSGAILGPLL
                     AFLLLSLKPDSYRFIFLLAALPGFLGMVVLILGIKEAGKKKESLIQRFKFRDFPKRFY
                     LFLAIIFIFTLGNSTDALLMVKANEVGVKVTFIPLVYLISSVVSVVLAIPFGVLSDRI
                     GREKLLVAGFLIYAGVYFGFGFTRNIDTIVILFALYGVYSASTDGVQKALISDLTAKN
                     KKGTGLGIYHALIGITLLPASLIAGTLYDKINSSIPFYFGSATAVLSALLMMVFV"
     gene            1760..>3022
                     /locus_tag="PLI30_12180"
     CDS             1760..>3022
                     /locus_tag="PLI30_12180"
                     /inference="COORDINATES: protein motif:HMM:NF013584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase family protein"
                     /protein_id="HOI80346.1"
                     /translation="MKTLRSGLKNVFFSFLLMGLMGCNSLAEKDPKIDLEEITIADLQ
                     QGYKTGKFTIVGITQAYLDRIEAIDKNGPALNAVIRVNPDALEIAASLDEELRQGKSR
                     GAMHGIPVLLKDNIDTHDKMPNTAGSVIMKNSFPLQDAWVTRKLREAGAVIIGKTNLS
                     EWANFHSSYSSSGWSALGGQTKNPYDLTRNPCGSSSGSGVAVSANLTVIAIGTETDGS
                     IVCPANNNGIVGIKPTVGLVSRSGIIPISFTQDTPGPMARTVRDAAICLGIMTGVDPK
                     DSKTSGSEGKSLADYSPFLNTDGLKGKRVGYDKTLAGRNHHVDNVMMEAVEFIKNSGA
                     EVIEIQNIIAPEIENMEFEVMLYEFKDGLNKYFATLGENAAVRSVEELIKLQSADSTE
                     MEWFDHKLIELAQEKGPVDSDEYRKIVRD"
BASE COUNT          903 a          706 c          709 g          694 t
ORIGIN      
        1 ctcgtggaag tgatccgggg acagctcacg gcagacagcg ttttcgacag tgtatcgggc
       61 atcgcaaaaa acatcggtaa agtgcccgta actgtaaatg aagcgcccgg atttttggta
      121 aaccggctgc ttatacccat gataaacgag ggcatcggtg cactggccga aggtgttgcc
      181 tcgaaagaag agatcgatga ggccatgaag ctgggtgccg gccaccccat cggcccgctg
      241 gcactggccg atttaatcgg gttggatgtt tgtctggcta tcctggaggt gttacaccac
      301 gagtttggcg acgacaaata ccgcccgcat ccgttgttgc gcaaaatggt ccgcgccaac
      361 ctactgggaa gaaaaatcgg aaccggtttt tacgattacc aaaaataaaa ggagtnnnnn
      421 nnnnntcaca aataccatca tcaacaaggc agacaaaaca gcggtggccg atccaaaata
      481 aaacggtatg ctcgagttga ttttgtcgta tagtgttcct gcaatcagac ttgccggcaa
      541 caacgttatt ccgatcaaag catggtatat tcccaatcct gtgccttttt tatttttagc
      601 tgtcaggtcc gaaataagtg ctttctgcac tccgtcggtt gaagccgaat aaactccata
      661 aagagcaaac aggataacaa tggtgtctat gtttcgggta aatccaaatc caaaatagac
      721 cccggcataa atcagaaaac ccgcgacaag cagtttttct cggccgattc tatccgacag
      781 tacaccgaac ggaatggcca gtaccactga cacaacactt gaaatcaggt agaccaacgg
      841 gatgaaagta actttaacac ccacctcatt ggcttttacc atgagaagtg catcggtgga
      901 atttcccaac gtgaaaataa aaattatagc aaggaacaga taaaaacgtt taggaaagtc
      961 cctgaactta aatctttgta tcaagctctc tttctttttt cccgcttctt tgattcctaa
     1021 aatcagtacc accatgccca agaatcccgg taaggctgca agtaaaaaaa taaaccggta
     1081 gctgtcgggc ttaagggaaa gaagtaaaaa agccagcaaa ggcccaagaa tcgctccact
     1141 gttgtccatg gctttgtgta aaccgaaact tttccccgtt tcgttttttt ctgtcgaccc
     1201 cgctacaagg ctatcccgcg gagcagatcg aattcctttt cctacacgct cggcaaaccg
     1261 aaaaagcaac atctgcaatg gggtgacaac ggtagcgtaa agcggcaaga tgagtgaaga
     1321 caatccatag ccgattacca tgaaaggctt gtttctacct atcttatcgc tccagtatcc
     1381 tgaaaacgct ttcaacaggg aagccgtgct ctccgccact ccttcgatca gcgaaaggct
     1441 tgttttggac acgccgatgg acattaaaaa catcggtacg acggaataga ccattttcgt
     1501 ggatgtatcc gttaaaaaac tgactatccc cgcgtaaaat acattggaag aaaatccaaa
     1561 aatctttctt tgtgttgtta cttcttcagg cataatacaa ctccggcata taagaaaaac
     1621 attctttgca gaggatgctc actcttccct cataccccgg caattaaaat tgtttacagc
     1681 aacaataaaa acatacatcc tacgggtttg ttcattatgc gtaaacaaac tacctttgaa
     1741 aaaagaaagg gaatccgtta tgaaaacact tcgttccggc ttgaaaaacg tattttttag
     1801 ttttctgctg atggggctga tgggctgtaa cagtctggca gaaaaggatc caaaaattga
     1861 tttggaagaa ataaccattg ccgatcttca gcaaggatac aagaccggaa aatttaccat
     1921 cgtgggaatt acgcaagcgt acctcgaccg gattgaagcc attgacaaga acggaccggc
     1981 gttgaatgcg gtaatcaggg tcaatcccga tgcactggaa atcgctgcaa gcctcgatga
     2041 agagttaagg caaggaaaaa gccgcggggc aatgcacggc attccggtat tgctgaaaga
     2101 taatatcgat acgcacgata agatgcccaa cactgccggc tcggtcatta tgaaaaactc
     2161 tttcccattg caggatgcct gggtcacgag gaaactgcgc gaggcgggag cagttattat
     2221 cgggaaaacc aacctgagcg agtgggccaa cttccacagc agctattctt ccagcgggtg
     2281 gagtgcgctg ggtgggcaaa caaaaaaccc gtacgacctt acccggaatc cctgcggctc
     2341 aagctccgga tcgggagtag ccgtttctgc caacctcacc gttattgcta ttggtacgga
     2401 aacggacggc tccattgtct gccctgcgaa taacaacggt attgtaggta tcaagcccac
     2461 agtgggtcta gtcagccggt cgggtattat tcccatttcc ttcacgcagg atactcccgg
     2521 gccgatggcg cgaaccgtgc gtgacgcggc catttgcctg ggcataatga cgggagttga
     2581 cccaaaagac tctaaaacaa gcggctcaga aggaaaaagc cttgccgact attctccttt
     2641 tctgaatact gacggattga aagggaagcg ggttggatac gataaaacac tggccggcag
     2701 gaatcatcat gtcgacaacg tgatgatgga agccgttgaa ttcattaaaa acagcggagc
     2761 agaagtcatc gaaatacaaa acattatcgc tcccgaaatc gaaaacatgg aatttgaggt
     2821 catgctctac gagtttaagg acggactaaa caagtatttt gccactttgg gagagaacgc
     2881 cgcggtaagg agtgtggagg aactgattaa attacaatcc gccgacagca ccgaaatgga
     2941 atggttcgat cacaaattaa ttgaactggc gcaggaaaaa gggccggtcg attctgacga
     3001 atacagaaaa atagtacgag ac
//
LOCUS       DAOSDR010000379         3004 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrimonas sp. isolate AMR_MDS_1826
            NODE_11561_length_3004_cov_3.045439, whole genome shotgun sequence.
ACCESSION   DAOSDR010000379 DAOSDR010000000
VERSION     DAOSDR010000379.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863355
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrimonas sp. (wastewater metagenome)
  ORGANISM  Petrimonas sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Dysgonomonadaceae; Petrimonas.
REFERENCE   1  (bases 1 to 3004)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3004)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:10:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,720
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 35
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3004
                     /organism="Petrimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11561_length_3004_cov_3.045439"
                     /isolate="AMR_MDS_1826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2023866"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..200)
                     /locus_tag="PLI30_12185"
     CDS             complement(<1..200)
                     /locus_tag="PLI30_12185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI80347.1"
                     /translation="MSVPKVITVSAVSGEFSPLISEFVESLKKFSIQAKKTEKKGWIQ
                     LVRNTAYHNPEDPNEPKVRVVPA"
     gene            complement(372..2036)
                     /locus_tag="PLI30_12190"
     CDS             complement(372..2036)
                     /locus_tag="PLI30_12190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI80348.1"
                     /translation="MKQFIYLLFTMAFFSITFINCEGDMLVIEEPEAGTEIASVTRYS
                     TPLSCYYVTTKGDDTNSGSSEEKAWRTIKYAASIAKAGDTVYIKAGEYLDEHVVIVNS
                     GTETEPIVFEGYRNRPGDKPQLKYDPLKNNKLDATIMPLLNGNDTTGRAFSITGKKFI
                     EIKNIQITLYERGINCTQRDPSCDYITLDNIIGTDFGFTSPNNSIKATRYSGLGIQIY
                     GNNCKILNCIIVDAGGENFGIRGDYHLVDKCESYSLTNHNPPDYFLHIGGDYNTIKNC
                     KANNLGNNAPYGHGIGFKGDCKYNKVIDCVAENLQEDFLVRHPECQYNEFINCTGIKH
                     NAFVIRDNASNNTFRNCKSKDAWASVLFWDSAEPSGQDGGGFNNTIVNCIFETSSTDP
                     CIVFSNDTYPSNAQGNIFRNCVFSKGSKLFSGGSDLDKGNIMKNCIVTDVKALGENPL
                     DFGIDITYSNFWKNGFKTPSGSGNMSKNPFFGGTSDFHLKSKYGRWNGTTWVKDHVTS
                     PCIDAGDPADEYSKEPSPNGKKINIGAYGNTSEASKSTVKKKIVKY"
     gene            complement(2071..2439)
                     /locus_tag="PLI30_12195"
     CDS             complement(2071..2439)
                     /locus_tag="PLI30_12195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI80349.1"
                     /translation="MKSRVLFIIIVLMIAATGIYFNIQGKERPTEKKSLIAASYFSGF
                     DLGNDKETLYYLTSGTIYENGRRVKGRDKISMGYEDVTRPTYVNSIALDKDGNVYTLA
                     RFDHEGKEVEDLVKIPNPFK"
     gene            complement(2647..>3004)
                     /locus_tag="PLI30_12200"
     CDS             complement(2647..>3004)
                     /locus_tag="PLI30_12200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI80350.1"
                     /translation="HWSVEPVSLPLDSVKAGKVLWERYRAGKKVDLQCLTVGDKARIL
                     FMPGELLVEYQLAAKKMRFRLFVAMAAYGDLGFGYIPPAAAFPQGGYEVEVAKVTPEA
                     EKVLLNAISNLLNDKH"
BASE COUNT          850 a          648 c          527 g          979 t
ORIGIN      
        1 gctggaacca ccctcacctt gggctcgttg ggatcttcgg gattgtggta ggcggtgttc
       61 ctaactaatt gtatccatcc ctttttctct gttttctttg cttgtatgga aaattttttc
      121 agactttcta cgaattcgga aataagagga ctgaattctc ccgatacggc cgagacggtg
      181 atcactttcg gaacgctcaa tttcccctct ttttgttcat aatgattagg ataggggatt
      241 atttgttgag cattgcataa aataaatgaa aaagataaaa ggagaaatag aattaatcgg
      301 gcacgcataa tatagttggg tttaatttta tataataaat tctttcattg ttgaaaataa
      361 gaagttacgt ctcaatattt tacaattttc ttcttaactg tactttttga agcttcactt
      421 gtattaccat aagctccgat gttaattttt tttccatttg gggatggttc ctttgaatat
      481 tcatccgcgg gatccccagc atcaatacac gggcttgtta catggtcttt aacccacgtt
      541 gttccgttcc atcgcccata tttcgatttg agatgaaaat cagacgtccc gccaaaaaac
      601 ggattcttgg acatgttccc tgatccggaa ggagttttaa aaccattctt ccagaaattg
      661 gaataagtaa tgtcaatacc aaaatccaaa ggattttcac ctaaagcctt aacatccgtt
      721 acaatgcaat ttttcataat gttaccttta tccagatcac ttcctcctga aaaaagctta
      781 cttccttttg aaaaaacaca gtttctgaaa atattacctt gtgcattgga cgggtatgta
      841 tcattactga aaacaatgca aggatctgtc gacgaagttt caaatataca gttgacgata
      901 gtattattga acccacctcc gtcttgtcct gacggttccg cactgtccca aaaaagaacg
      961 gaagcccatg catctttcga cttacaattt ctaaacgtat tattgctggc attgtctcga
     1021 attacaaaag cattgtgttt aataccggta caatttatga actcgttgta ttggcattca
     1081 gggtgtctaa cgagaaaatc ctcctgcaaa ttttcagcaa cacagtctat aactttgtta
     1141 tacttgcaat ctcccttaaa tccgataccg tgtccgtaag gagcattatt tcctaagttg
     1201 tttgctttac agtttttgat agtattataa tcaccgccta tatgtaaaaa ataatcagga
     1261 ggattatgat ttgttaggct ataggattcg catttatcta ctagatggta atcacccctg
     1321 attccaaagt tttctccacc agcatcaact attatacaat taagtatttt gcaattattg
     1381 ccataaatct gaattccaag tcctgaatat ctggtggctt tgattgaatt attgggcgat
     1441 gtaaatccga aatcggttcc gattatatta tctagtgtaa tataatcaca tgagggatcc
     1501 ctctgggtac aattgattcc tctttcatat agagtaatct gaatattttt tatttcaata
     1561 aattttttcc cggttattga aaaagcacga cctgtcgtat cgtttccgtt taacaaaggc
     1621 attatagtcg catcaagttt gttgttctta agtggatcat atttcaactg tggtttgtca
     1681 cccggacgat tgcgataacc ttcaaaaacg attggttccg tttcggtgcc tgaattgaca
     1741 ataactacgt gctcatccag atattcaccc gctttaatat aaacggtatc accagcctta
     1801 gcaattgacg ctgcgtattt aattgtacgc caagctttct cttccgagct gcctgaatta
     1861 gtgtcgtctc ctttagttgt tacataataa catgagagtg gcgtggaata cctggttacc
     1921 gaagctattt ctgttcctgc ttctggttct tcgataacta gcatatcacc ttcacaattt
     1981 atgaacgtta tagaaaaaaa cgccatagta aataatagat aaataaattg tttcatagtc
     2041 ttcatgaaat gtttaaatat ttttcaataa tcatttaaac ggatttggta tttttaccag
     2101 atcctctact tctttaccct catggtcaaa tcttgctagg gtgtacacat taccatcctt
     2161 gtcaagagcg attgaattga catacgtagg gcgtgtgacg tcttcatacc ccatcgatat
     2221 cttatcccgt cccttcactc ttctgccgtt ttcataaata gtcccgctgg tcaggtaata
     2281 cagggtctcc ttgtcgtttc ccagatcgaa gcctgaaaaa taagaagcgg ctatcaacga
     2341 tttcttttct gtcggacgct ccttcccttg tatgttaaaa tagatacccg ttgctgctat
     2401 catcaaaaca atgatgataa ataaaactcg acttttcatt accattccat acattttttt
     2461 taggtcatac attttaaaat aatagtccca aactgagatc gctacgcgct ttcttagcta
     2521 cataacagca ttggtttggc ggaaaaatga tttcgcttcc attttcccaa ttgatgctgt
     2581 attgcagtga cagaataaga ggaattaatg ttctgtcagg gtagaattat cttatttgtt
     2641 gatgattcaa tgcttgtcat tcaacaagtt ggaaatagca ttcaaaagta ctttttccgc
     2701 ttccggcgtc actttcgcca cctccacttc ataaccgccc tgtggaaaag ccgcggccgg
     2761 aggaatatac ccaaatccca gatcgccata cgccgccatg gcaacaaaca gacggaaacg
     2821 cattttcttg gcggcaagct ggtactcgac caacaactct cccggcataa aaagaatgcg
     2881 cgccttatcg ccgaccgtca agcactgcaa atcgactttc ttccccgcac ggtaccgttc
     2941 ccaaagcacc ttgcctgcct ttaccgaatc cagcggtaaa gaaaccggtt caacactcca
     3001 gtga
//
LOCUS       DAOSDR010000380         2996 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrimonas sp. isolate AMR_MDS_1826
            NODE_11595_length_2996_cov_4.580755, whole genome shotgun sequence.
ACCESSION   DAOSDR010000380 DAOSDR010000000
VERSION     DAOSDR010000380.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863355
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrimonas sp. (wastewater metagenome)
  ORGANISM  Petrimonas sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Dysgonomonadaceae; Petrimonas.
REFERENCE   1  (bases 1 to 2996)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2996)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:10:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,720
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 35
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2996
                     /organism="Petrimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11595_length_2996_cov_4.580755"
                     /isolate="AMR_MDS_1826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2023866"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            21..722
                     /locus_tag="PLI30_12205"
     CDS             21..722
                     /locus_tag="PLI30_12205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007652918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HOI80351.1"
                     /translation="MGRKAKEIIDLSSTLADIWRVLSEEEREQIKKNARFVHYKKNET
                     IYIEEEQPLDLMCLLKGKVKIYKSGVGGRTQIIRMIRPIQYFGYRAFFAREPYVTAAS
                     AFEASTVCFISMELIEDMLRENGNLALFFIQMLSCDLGVADNRVVNLTQKHVRGRLAE
                     SLIFLKESYGLEEDGATINIYLAREDLANLSNMTTANAIRTLANFVNERIISLDGRKI
                     KIIDEDKLLKISKIG"
     gene            731..1363
                     /locus_tag="PLI30_12210"
     CDS             731..1363
                     /locus_tag="PLI30_12210"
                     /inference="COORDINATES: protein motif:HMM:NF027595.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4923 family protein"
                     /protein_id="HOI80352.1"
                     /translation="MNAFNIPLIKKPQVVVFIFSLSTLFFSVSAQEKVESKNVFQQVW
                     SLFANSSLSDKDLIGDWGYKGTACTFETENLLKKAGGAVVASQVEKTFNEYCMKIGIS
                     EEKCDFTFKEDSTYSAKLGLLKLSGKFRLDEEKKVLSMSYMRGLGRMTAHVHRSGSGL
                     KLLFDADSFLQMMKTLSMFTKNNSVEILAAMADLYDGMLLGFDLEMKKQP"
     gene            1464..2975
                     /locus_tag="PLI30_12215"
     CDS             1464..2975
                     /locus_tag="PLI30_12215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019539717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GH3 auxin-responsive promoter family protein"
                     /protein_id="HOI80353.1"
                     /translation="MIKQITRAVFKSYYKSVEKFIDHPVATQEKIFSYLLEHGRNTLY
                     GAENQFGDILTVSDFQKKVPVTSYEDLRPYLDKIIRDRQQNVLWDSPVKWFAMSSGTT
                     EDKSKYIPVTQESLEHCHYRCGYHMLGLYTNNYPDTEFFLGKTLVLGGSQQINNIGDS
                     TFTGDISAILMKNLPFIAKLARTPEEIALLPDWEEKLKKLSDYAVKEDIRAFLGVPSW
                     MMVLLKKIVEDTGKPLPDLWKNLEVFFHGGVSFGPFREQYEKLIPTKKMRYWETYNAS
                     EGFFGVQYSSKSNDMLLLLDNEVFYEFLPAEEWDKECSQALTLADVEIGKQYALVIST
                     SGGLWRYKIGDTIEFTSTNPYLFRITGRTKQFINAFGEELIVDNADRALTEACRITGA
                     KVTEYTVAPIYFSDNHGGAHEWLIEFEDKPSDLKQFESVLDENLKKLNSDYEAKRSYN
                     LSLGKPVVRMLEPGDFYKWMKFREKTGGQNKVPKLSNDRKYVESILSVLGIKS"
BASE COUNT          870 a          569 c          723 g          834 t
ORIGIN      
        1 acatagtttt gtaacatgtg atgggcagaa aagcaaagga aataattgat ttatcatcca
       61 ctttggcaga tatctggcgt gtgctctcgg aagaggaacg tgaacagatc aagaaaaatg
      121 cccggtttgt tcattataag aaaaatgaaa ctatatacat tgaagaggaa caacccctcg
      181 atttgatgtg tctgcttaag gggaaagtga aaatttataa gagcggcgta ggtggaagga
      241 cgcagattat ccggatgatc cgccccatcc agtatttcgg ctaccgcgct ttttttgcac
      301 gggaacctta tgttactgcc gcttcggcct ttgaggcttc aaccgtttgt tttatttcga
      361 tggagctgat cgaagatatg ttaagagaaa acggtaacct ggcactgttt ttcatccaga
      421 tgctgtcttg tgatctgggg gtcgccgata accgggtggt caatcttacc caaaaacacg
      481 tcaggggaag gcttgctgaa tcgcttatct tcctgaaaga aagctacggc ctggaagaag
      541 acggtgcaac cataaatatt tatctggctc gcgaagatct ggccaatctt tccaacatga
      601 ccacagccaa tgctatcaga acgctcgcca actttgtgaa cgagcgcatc atttctctcg
      661 acgggagaaa gataaaaata atcgatgagg ataaattatt gaaaataagc aaaataggat
      721 agtggcgctg atgaatgcct ttaacatacc cctgattaaa aaaccacaag ttgtcgtttt
      781 tattttctca ttgtctaccc tctttttcag cgtttcggct caggaaaaag tggaatcgaa
      841 gaacgtgttt cagcaagtat ggagcctgtt tgcaaattca tcattgtcgg ataaagacct
      901 gataggtgac tggggatata agggtactgc ctgtacgttc gaaaccgaga acctgttgaa
      961 aaaagccggc ggtgcagtag tagcctcaca agtagagaaa acgtttaacg aatactgtat
     1021 gaagatcggg atcagcgagg agaagtgcga ttttactttt aaggaggaca gcacttactc
     1081 tgcgaaactg ggtttattga agctttcggg taagtttaga ttagacgaag aaaagaaagt
     1141 cctttccatg agttatatgc gtggtttggg acgtatgacg gcccatgttc accggtcggg
     1201 ttcggggtta aaactgctgt ttgatgccga tagttttctt caaatgatga aaaccctgag
     1261 catgttcacc aagaataatt ccgttgaaat tctggctgcc atggctgatc tctacgacgg
     1321 tatgttgttg ggttttgact tggagatgaa aaaacagcct tgaccttctt tttacttttt
     1381 ttatgttttc aaattttttt aatggtcaat tttcaaaaag tccttcttac ctttgtgccg
     1441 tcaaatcata aacatgattg tggatgatca agcaaattac aagagccgtt tttaaatcct
     1501 attataagtc agtcgaaaaa ttcatcgacc atcccgtcgc tacgcaagag aaaatatttt
     1561 catacctgtt ggagcacgga cgaaatacgc tttacggtgc agaaaatcag tttggggata
     1621 ttcttacggt ttccgatttt cagaaaaaag ttcctgttac ttcttatgaa gatttacggc
     1681 cttatcttga taaaataatt agagacaggc agcaaaatgt gctttgggac tcgcctgtaa
     1741 agtggtttgc catgtcgagc ggaaccactg aagacaagag caaatatatt cccgtaacac
     1801 aggagtcgtt ggagcattgt cattacaggt gcggatatca tatgcttggc ctgtacacaa
     1861 acaactatcc cgatacggag ttttttcttg gcaaaacgct ggtgttggga ggaagtcagc
     1921 aaattaacaa tattggtgac agcacgttca ccggtgatat ttctgctatc ttgatgaaaa
     1981 acctcccttt tatagccaag ctggcacgaa cacccgaaga gattgcctta cttcccgatt
     2041 gggaggaaaa attgaagaaa ctgtcggatt acgcagtaaa ggaagatatc cgggcatttt
     2101 tgggagtccc gtcgtggatg atggtgttgt tgaaaaaaat tgtggaggat accggcaagc
     2161 cacttcccga cctgtggaaa aatttagagg tattctttca cgggggagtg agtttcggcc
     2221 cgtttcgcga gcaatatgaa aaactgattc ctacgaagaa aatgcgttac tgggaaacat
     2281 acaatgcctc tgaagggttc tttggcgttc agtattcgag caaatccaac gatatgttgc
     2341 tgttgctgga taatgaggtt ttctacgaat ttcttcccgc ggaagagtgg gacaaagagt
     2401 gttctcaggc tctgacgctg gctgatgtgg agattgggaa gcagtacgcg ttggtgattt
     2461 caactagcgg gggcttgtgg cgttacaaaa taggcgacac catcgaattt acctccacca
     2521 atccgtattt attcaggatt acgggtcgca ccaagcagtt tattaacgct tttggtgaag
     2581 aactgattgt ggataatgcc gatcgtgctt tgacagaggc ctgccggatt accggagcta
     2641 aggtgaccga atacacggta gcccctatct acttcagcga taatcacggg ggagcacacg
     2701 aatggcttat cgagtttgaa gataaaccgt ctgatttgaa gcaatttgaa tctgttttgg
     2761 atgaaaacct gaaaaagctg aactccgatt acgaggccaa acgctcatac aacctgtctc
     2821 ttggtaagcc tgtagtgcgt atgctggaac cgggagattt ttataaatgg atgaaattca
     2881 gggagaaaac gggtgggcaa aataaagttc ccaaactatc gaacgacaga aagtatgttg
     2941 aaagtatttt gtccgtgttg ggtataaaat cctaaaatag aataaaattg gctttc
//
LOCUS       DAOSDR010000381         2991 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrimonas sp. isolate AMR_MDS_1826
            NODE_11617_length_2991_cov_4.722411, whole genome shotgun sequence.
ACCESSION   DAOSDR010000381 DAOSDR010000000
VERSION     DAOSDR010000381.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863355
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrimonas sp. (wastewater metagenome)
  ORGANISM  Petrimonas sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Dysgonomonadaceae; Petrimonas.
REFERENCE   1  (bases 1 to 2991)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2991)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:10:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,720
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 35
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2991
                     /organism="Petrimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11617_length_2991_cov_4.722411"
                     /isolate="AMR_MDS_1826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2023866"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1081
                     /gene="murF"
                     /locus_tag="PLI30_12220"
     CDS             <1..1081
                     /gene="murF"
                     /locus_tag="PLI30_12220"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019538670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HOI80354.1"
                     /translation="LQKLANFHRKKLKITIVGITGTNGKTTTKELIASILSREFKVAF
                     TQGNFNNHIGVPLTLLSINKSHEIGVIEMGANHPGEIRELCEIAEPNIGLITNIGKAH
                     IEGFGSLENVIKTKCELYDFIREHEGKVFVNKDNSTLSEKSEGMDRILYGRDNPGLFV
                     SGTLSAETPFLHFDWNFFEHPHRVKTHLVGEYNLDNALAAAAIGKYFGINAELISSAL
                     EAYEPKNNRSQFERTQHNDLIIDAYNANPTSMKAMLEFFSKIKSDLPKAVILGEMKEL
                     GPIAEVEHRKMLDYLHGQSFDKIYLVGSVFTDGVTGSITMKNTFVFERVEQLIEELER
                     HPLAGYYVLLKGSHSVQLEKVIPFL"
     gene            1104..1946
                     /locus_tag="PLI30_12225"
     CDS             1104..1946
                     /locus_tag="PLI30_12225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI80355.1"
                     /translation="MIHYLQCNNCGNRNEMKSEYVVFCTSCGQKLDNNFTQWQRRNPG
                     GTFEDFKRNACFTEDQLPPAIPIKKGKILKSRSLKEKILIVVITTIFAAVGSWLGSAA
                     VTALKNNKKTDEKVLSEQWVKKTYGTYQLTLETPWELKSSTPPPLPENIKRMLEKMET
                     FETSNDNQFKVGVIEAKYNPAEIGELNMQGAADGAVNEVKGMQGVTNFVYSEDSYSVR
                     NLPGFIQRGTYTQNGYHIAFINIGIVKDLNYWQVMVLSRRDDKVAEQAAERIIQSIQI
                     NYPS"
     gene            1991..2548
                     /locus_tag="PLI30_12230"
     CDS             1991..2548
                     /locus_tag="PLI30_12230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI80356.1"
                     /translation="MKSLIVAALILFGCFSFNAHAQTYKINKQKYDYKMYMPQPGDPN
                     NPAVMGLASFFIPGLGQMLSGETGRGLAFLGGSIALSGITVAGALMSYDEVTTYNQFG
                     SFTEYETNPAGVAIMLTGLAATIALDVWAIVDAVRVAKVNNMYIQDLRGNLSSVKVEL
                     NPFIDTHNYLGQANTSAGLSLKVTF"
     gene            complement(2552..>2991)
                     /locus_tag="PLI30_12235"
     CDS             complement(2552..>2991)
                     /locus_tag="PLI30_12235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006843353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI80357.1"
                     /translation="EAERKIQWLIDAGIKMLRDSAVLIDSAFYVVGREDFVFSDRLPL
                     SEILNRQNVNVSKPVIVLNHSPNDLDEEVNAGADIALYGHTHHGQAFPGNIATRLVFE
                     VAYGYARKGNTHIYVTSGLGLAGPQYRIGTVSEVVVLNVKFEK"
BASE COUNT          911 a          620 c          673 g          787 t
ORIGIN      
        1 tttgcaaaag ttggccaatt ttcaccggaa aaagctaaag ataaccattg tgggtattac
       61 gggtacaaat ggaaagacga ccacgaagga gttgattgcc tctatccttt ccagggagtt
      121 taaggttgct tttacccagg gaaacttcaa taaccacatc ggtgtgcctc tcacattatt
      181 gtcgataaat aagtcgcacg aaattggtgt cattgagatg ggcgccaatc atccaggcga
      241 aatccgtgaa ttatgtgaga tcgccgaacc caatatcgga ttgatcacca acatcggaaa
      301 agcccacatt gaaggattcg ggtcgttgga aaatgtaatc aagaccaaat gcgaacttta
      361 cgattttatc cgtgaacacg aaggcaaggt gtttgtgaac aaagataatt caacgttgag
      421 cgaaaaatcg gaaggaatgg acaggatttt gtacggacgt gataaccctg gcttgtttgt
      481 gtcgggtaca ttgtcggctg aaacaccatt cttgcatttc gattggaatt tttttgaaca
      541 ccctcaccgt gtgaaaactc atctcgtggg agagtataac ctcgataatg ccctggctgc
      601 tgcagccata ggtaaatatt ttggcatcaa tgccgagctt atcagtagcg ccctcgaagc
      661 ttacgagcct aaaaacaacc gttcgcaatt tgaacgtact cagcacaacg accttattat
      721 tgatgcttac aatgctaacc ccacaagcat gaaagccatg cttgaatttt tctcaaaaat
      781 caaatccgat ttacctaagg cggtcatttt gggtgagatg aaagaattgg gacctatagc
      841 cgaagttgaa cacaggaaaa tgttggacta tctgcacggg cagtcattcg ataaaattta
      901 cctggttggt tctgtattta ccgacggagt aactgggagc atcacaatga aaaatacgtt
      961 cgtttttgag cgagtggaac aattgattga ggagttggaa cgacatcctt tagccgggta
     1021 ttatgtcctg ctaaaagggt cacactcggt ccaactggag aaagttatac catttttgta
     1081 aatttgtaaa gaagaaacac gacatgatac actacctaca atgcaacaac tgcggcaatc
     1141 gtaacgagat gaaatcggaa tatgtggtat tctgtacatc gtgcggacag aagctcgaca
     1201 ataatttcac ccaatggcag cgaagaaatc ccggcggaac atttgaagat tttaaacgaa
     1261 acgcctgttt cacagaagat caattgccac ccgcaatacc gataaagaaa ggcaagatac
     1321 tcaaatcgag atctctgaag gagaaaattc ttatcgtagt aataaccaca atttttgcgg
     1381 ctgtcggttc atggcttggt agtgcggctg tcactgcact taagaacaac aaaaaaaccg
     1441 atgaaaaggt actctcggaa caatgggtaa agaaaaccta cggaacttat cagttaactc
     1501 ttgaaacacc ctgggagctg aaatcttcga caccaccccc gttgcccgag aatataaaaa
     1561 gaatgctgga gaagatggaa acatttgaaa cgtccaatga caatcaattt aaggtagggg
     1621 tgattgaggc aaaatacaat ccagccgaga ttggcgaact gaatatgcaa ggtgctgctg
     1681 acggtgcagt taacgaagtt aagggtatgc aaggagttac caacttcgta tattcagaag
     1741 acagttattc tgtccgtaac cttcccggat ttatccagcg agggacatat acccagaacg
     1801 gatatcacat tgcttttata aatataggaa tagtgaagga tttaaattat tggcaagtga
     1861 tggttttaag cagaagagac gataaagttg cagaacaggc agcagaaaga attatccaat
     1921 ccatacaaat caattatccg tcttagcgga ttaaaacaat aaagagcata tttttaaaaa
     1981 aatcgatatt atgaaatcat taattgttgc agcattaatc cttttcggat gcttttcttt
     2041 taatgcgcat gcgcaaacct acaaaatcaa caaacaaaaa tacgattata aaatgtacat
     2101 gccacaaccc ggagatccga ataatcccgc ggttatgggg cttgcctctt tctttattcc
     2161 gggattagga cagatgctta gtggggaaac aggtcgtggc cttgcttttt taggaggttc
     2221 aatagctctg tcaggcatta ctgttgccgg agcattaatg tcgtacgatg aagttacaac
     2281 ctacaaccag tttggatctt tcacggagta tgaaacaaac cctgccggtg tggcgataat
     2341 gcttaccggg ttggctgcta ccatcgcgct cgatgtatgg gctattgtcg atgcggtaag
     2401 ggtggcaaaa gtaaacaata tgtatattca ggatcttcgc gggaacttga gttctgtaaa
     2461 agtcgaattg aatcctttta ttgatacgca caactatctg ggacaggcca acacttctgc
     2521 cggcttgtcg ctgaaagtta cgttttgaaa cttacttctc aaatttcaca ttcaatacca
     2581 ctacttccga aaccgtgcct atgcggtatt gcgggccagc taatcccagt cccgaggtta
     2641 catagatatg tgtgttccct ttccgggcat acccgtatgc cacttcaaaa actaagcgtg
     2701 tggcaatatt tccgggaaaa gcctgtccgt ggtgggtgtg tccgtatagc gcaatgtcgg
     2761 ctccggcatt gacctcttcg tcaaggtcgt tggggctgtg gttcaggacg atgaccggtt
     2821 ttgatacatt cacgttttgc cggttcaaaa tttcggataa aggcaaccgg tcggagaaca
     2881 caaaatcttc acgtccaacc acataaaaag cgctgtcaat caggacagcc gaatcgcgca
     2941 gcatttttat gcccgcatcg atcaaccatt gtatttttcg ttcggcttcg t
//
LOCUS       DAOSDR010000382         2988 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrimonas sp. isolate AMR_MDS_1826
            NODE_11638_length_2988_cov_4.533242, whole genome shotgun sequence.
ACCESSION   DAOSDR010000382 DAOSDR010000000
VERSION     DAOSDR010000382.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863355
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrimonas sp. (wastewater metagenome)
  ORGANISM  Petrimonas sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Dysgonomonadaceae; Petrimonas.
REFERENCE   1  (bases 1 to 2988)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2988)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:10:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,720
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 35
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2988
                     /organism="Petrimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11638_length_2988_cov_4.533242"
                     /isolate="AMR_MDS_1826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2023866"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1252)
                     /gene="tyrS"
                     /locus_tag="PLI30_12240"
     CDS             complement(<1..1252)
                     /gene="tyrS"
                     /locus_tag="PLI30_12240"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012457449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HOI80358.1"
                     /translation="MNFVEELRWRGMIHDVMPGTEEQLLKEQTSGYLGIDPTADSLHI
                     GHLVGVMMLKHLQRSGHTPIALVGGATGMIGDPSMKSAERNLLDENTLRHNQEAIKKQ
                     LAKFLDFESDIPNKAILVNNYDWMKNFSFLSFIRDIGKHITVNYMMAKDSVKKRLSGE
                     ASEGMSFTEFSYQLMQGYDFLHLYREYGCRLQMGGSDQWGNITTGTELIRRIAGGEAF
                     ALVCPLITKADGGKFGKTESGNVWLDSRYTTPYQFYQFWLNVSDADAEKYIKIFTALS
                     REEIESLVAEQQSAPHLRPLQKRLAEEITVMVHSREDYDMAVSASEILFGKSTSHALR
                     SIDETTFLQVFDGVPQFNISRSLVTEGIKAVDLLTEAAAVFPSKGEMRKTVQAGGVSI
                     NKDKIDDFEAVIDESHLIADKYILA"
     gene            complement(1448..2893)
                     /locus_tag="PLI30_12245"
     CDS             complement(1448..2893)
                     /locus_tag="PLI30_12245"
                     /inference="COORDINATES: protein motif:HMM:NF014443.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HOI80359.1"
                     /translation="MTRWEVIVKYLGYVLLFNAFFFYISAAISFFLHENSLTPLLYSA
                     VICTILGSFPQIFVERIEEINFREGLSISVLGWILTCFAGAVPYFMWGGEFTFANALF
                     ESVSGYTTTGATILRDVEALTKGLLFWRASTHFIGGVGIILFVLLILPSATGTRSSIY
                     NSELSGLSKLNFKMRTRQIARVIAVVYLSLIIIEIALLWVLGMNFFDAICHAFATVAT
                     GGFSTKNLSVAYYDSVWIEVVIMTFMLLGSIHFGLLYGTVTGNKMNVFTSKSVRTYIG
                     LMFLGIIVVAAKLTNDGFYSWWTSLRYASFQVISLGTTTGFATVDTPNWPILTIIIIM
                     YFTIQCGMVGSTSGGLKFDRVLLFYQSLKRQIKLVLHPSGVYITKLENVVISTEMELQ
                     TAIFFILYLVTLFVTALLLSSMNVDGMTSISASIATIGNAGPGFGKVSSLGNYSSIPD
                     AGKYVLSINMLLGRLEIINVFALFTVLTYKK"
BASE COUNT          883 a          667 c          609 g          829 t
ORIGIN      
        1 gtgccaggat atatttatcg gcaataaggt gagattcatc tataactgct tcaaaatcat
       61 caattttgtc tttatttatc gatacaccac cggcctgtac ggttttccgc atctcaccct
      121 tagatgggaa aacggcagcc gcttctgtta ataaatccac cgcttttata ccttcagtaa
      181 ccagacttct tgaaatattg aattggggaa ccccatcgaa aacctgaaga aaggtggttt
      241 cgtcaataga acgcaaggca tgagaggtgg atttcccgaa aagaatttcc gatgcactaa
      301 cggccatgtc gtagtcttca cgactgtgaa ccataacggt gatttcctct gccagacgtt
      361 tctgcaacgg gcgcaaatgc ggggcagact gttgctctgc caccagagat tcaatctctt
      421 ccctcgataa tgccgtgaaa atcttgatat atttttcggc atcggcatcg ctcacgttca
      481 gccagaactg atagaactgg taaggtgtcg tataacggct gtccagccat acatttccgc
      541 tttcggtttt accgaatttt ccgccgtcgg ctttagtaat gagtggacaa accagcgcaa
      601 aagcttctcc gcctgcaatc cggcggataa gttcggtccc ggtagtgatg ttaccccatt
      661 gatcgcttcc acccatttgc aagcggcatc cgtactcacg atacaggtga aggaagtcgt
      721 atccttgcat caactggtaa gaaaattcgg tgaaggacat gccttcactg gcttcaccgc
      781 tcagacgttt ctttacggaa tctttcgcca tcatgtaatt aaccgtgatg tgcttgccga
      841 tatcgcgaat aaacgaaagg aacgaaaaat tcttcatcca gtcgtaattg tttaccaaaa
      901 tagctttgtt gggaatatcg ctttcaaaat cgaggaattt ggccagttgt ttttttatcg
      961 cttcctggtt atgccgcaag gtgttttcgt ccaataggtt acgttctgcc gatttcatcg
     1021 atgggtcacc gatcattcct gtggctccgc caacaagcgc tattggtgta tgtcccgagc
     1081 gttgcaggtg tttcagcatc atcacgccca ctaagtgtcc gatatggaga gagtcggctg
     1141 tggggtcaat tcccaggtac cccgacgttt gttctttcaa taactgttct tccgttccgg
     1201 gcatcacatc gtggatcatg cctcgccaac gaagttcttc tacaaagttc atgttcattc
     1261 agttgtaatt cgattgtaat tcagtaaata tgcctaatag caattttcaa ttcaatggaa
     1321 atttgttgtt ccggtgaaca tttgtttgcc gcaattaatt actgttgaat aatgcgtagc
     1381 gaatttcaac gaagctaaat aaccattgaa tagggtgcaa agatacattt ttcttcccag
     1441 aaaacggtta ttttttgtag gttaacaccg taaacaaggc aaagacgtta ataatttcaa
     1501 gacgtcccaa cagcatgttt atcgataaaa catattttcc cgcatcgggg atggatgagt
     1561 aatttccaag tgaacttact tttccaaaac caggtcctgc attaccaatt gtagcaatgg
     1621 aagccgatat ggaagtcata ccatcgacat tcatgctgga gagcaagaga gccgtaacaa
     1681 acagcgtaac cagataaaga ataaagaaaa tggcagtttg taactccatt tcggtgctga
     1741 tgacgacatt ctccagtttc gtaatatata cgccgctcgg atgcaagaca agttttatct
     1801 gccgtttcag tgattgataa aaaagcaata cacgatcaaa ttttaatcct cctgacgtgg
     1861 agccgaccat tccacactga atcgtaaaat acatgataat gataatggta agtatcggcc
     1921 agtttggagt gtccacagta gcaaagccgg tagttgttcc cagagatatt acttgaaacg
     1981 aagcgtaccg gagggatgtc caccacgagt aaaacccgtc gttggttaac tttgcagcaa
     2041 caacaatgat tcccaaaaac attaaaccta tataagtcct gacggacttt gacgtgaata
     2101 cattcatttt gttccccgtt acggtaccgt acaataatcc gaaatgaatg ctccccaaca
     2161 gcatgaatgt cataatgacg acttctatcc aaacactgtc gtaatatgcc acgcttaagt
     2221 ttttggttga aaaaccaccc gttgccactg ttgcaaaagc atgacagata gcatcaaaga
     2281 aattcatacc caatacccaa agaagtgcaa tttcgataat gatcaggcta agatagacaa
     2341 cggcgataac acgcgctatt tggcgtgttc tcattttaaa gtttaattta gacaatccgg
     2401 atagttccga gttgtaaata ctcgatcttg taccggtggc actgggtaaa atcaatagaa
     2461 caaacagaat gattcccaca ccgccaataa aatgtgtgga tgcccgccaa aacaacaatc
     2521 ccttggtcaa tgcttctaca tctctcaaaa tggttgcccc ggtagtggta tatccagaaa
     2581 cactttcgaa aagggcattg gcaaatgtga attcgccgcc ccacatgaaa tagggtaccg
     2641 ccccggcaaa gcaggttaga atccatccta aaacactgat tgacaatcct tctcggaagt
     2701 tgatttcttc aattctttcc acaaatatct gtggaaaaga gccaaggatt gtgcagatca
     2761 ctgcactgta tagaagcgga gtgagtgaat tttcgtgaag gaagaatgaa attgcggcgg
     2821 aaatgtagaa aaaaaacgcg ttaaataaca gcacgtatcc taaatatttt acaattactt
     2881 cccaacgggt cattttattt gtttttatct gttagagaaa caagcaacac aatcacattc
     2941 ataatttcca aacgccccag cagcatatta gtcgaaaaaa tgtatttt
//
LOCUS       DAOSDR010000383         2981 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrimonas sp. isolate AMR_MDS_1826
            NODE_11668_length_2981_cov_4.214627, whole genome shotgun sequence.
ACCESSION   DAOSDR010000383 DAOSDR010000000
VERSION     DAOSDR010000383.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863355
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrimonas sp. (wastewater metagenome)
  ORGANISM  Petrimonas sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Dysgonomonadaceae; Petrimonas.
REFERENCE   1  (bases 1 to 2981)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2981)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:10:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,720
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 35
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2981
                     /organism="Petrimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11668_length_2981_cov_4.214627"
                     /isolate="AMR_MDS_1826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2023866"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..829)
                     /locus_tag="PLI30_12250"
     CDS             complement(<1..829)
                     /locus_tag="PLI30_12250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002558998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lyase family protein"
                     /protein_id="HOI80360.1"
                     /translation="MAKIWNKGFDADKSVENFTVGKDREFDLRLAKYDIMGSMAHIKM
                     LVKIGLLKKTEEETLRLELQKILAEVEKGNFNLNTDAEDIHSQIEAMLTETLGETGKK
                     IHSGRSRNDQVLVDLKLFFKDEIRKIKADALQLFDLLQLLSEKHRSVLLPGYTHGQIA
                     MPSSFGLWFGAYAETLVDDMHTLVSAWRVANQNPLGSAAGYGSSFPLDREMTTRELDF
                     ETLNYNAVAAQMGRGKTERILAFSVASFASTLNKLAADNCMYLSGNYGFISYPDNLTT
                     "
     gene            complement(874..1941)
                     /locus_tag="PLI30_12255"
     CDS             complement(874..1941)
                     /locus_tag="PLI30_12255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018696491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HOI80361.1"
                     /translation="MNKEYIRRSANLLKELVSLKSFSGEEKVRSDFLCSYLSERGVET
                     ERSGNNIIARQPHHNMAKQTLMLNSHIDTVRPAATYTFDPFNPPLSDTHIFGLGSNDA
                     GASVVCLLQTFLHFYESELPFNLMLVLSCEEENSGPNGMTRLAKELPDIDFALIGEPT
                     GMQAAIAERGLLVIDGIASGVSGHAARDEGVNALYIALEDIQTMRSIKFDKISPTMGE
                     VKLTVTQISAGTQHNVVPDKCTFVVDIRPTDQYTNPEILQLLRVNVKSKLSARNLTNK
                     SSATPSDHLLIKAIGQLGVKTYVSPTTSDWMRISCPAIKMGPGESARSHQADEFVLVS
                     ELESGIAGYIRFIEEMTKIIK"
     gene            complement(1956..2747)
                     /gene="argB"
                     /locus_tag="PLI30_12260"
     CDS             complement(1956..2747)
                     /gene="argB"
                     /locus_tag="PLI30_12260"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019538708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HOI80362.1"
                     /translation="MSIHLNNSLSVVKIGGNIVDNPEALTGFLNDFHQLKGRKLLVHG
                     GGVMASKMAERLGIKTKMVEGRRVTDAETLQLVTMVYAGWINKSIVAALQKIGCNAIG
                     LSGVDANCIPSERRNPEPIDFGFVGDPITWKMDGKLLSTFIENDVIPVFCAITHDGNG
                     SLLNTNADTVASSLAVALSKHYRTTLYYCFEKEGVLRDINDKNSLIPLINREAFIQLK
                     GQGVIADGMIPKLDNSFNAIQEGVSEVVILHARNLLKKTGTRLIG"
     gene            complement(2767..>2981)
                     /locus_tag="PLI30_12265"
     CDS             complement(2767..>2981)
                     /locus_tag="PLI30_12265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009317864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="acetylornithine carbamoyltransferase"
                     /protein_id="HOI80363.1"
                     /translation="KILSTDRSWTVDTPKMALTNNAYFMHCLPVRRNMIVTDEVIESP
                     QSIVIPEAANRVVSAQTVLKEILLAL"
BASE COUNT          694 a          706 c          705 g          876 t
ORIGIN      
        1 cggtagtcag gttgtcaggg tatgagataa atccataatt cccagataga tacatgcagt
       61 tgtcggcagc cagcttatta agcgttgatg cgaacgacgc tacgctgaaa gcgaggatcc
      121 tttcggtttt tcctcttccc atctgtgctg caaccgcgtt gtaattcagg gtttcgaaat
      181 cgagttcacg tgtggtcatc tctctatcga gcgggaatga actaccgtat ccggcagcag
      241 aaccaagagg attctgattg gcaacccgcc acgcggaaac cagtgtatgc atatcgtcca
      301 cgagcgtttc ggcgtatgct ccgaaccaca gtccgaaaga tgatggcatt gctatttgtc
      361 cgtgggtata tccgggcagc aggaccgatc ggtgtttttc actcagcaac tgcaataagt
      421 cgaaaagctg caaggcatct gcttttattt tccggatttc gtccttgaag aaaagcttca
      481 gatccaccaa tacctgatcg tttcgcgagc gcccagaatg gatcttcttc cctgtttcac
      541 ccagcgtttc ggtgagcatt gcctctattt gcgaatggat atcttcagca tcggtgttga
      601 ggttgaaatt tcctttttcc acttcagcga ggatcttttg caattccagg cgcaatgttt
      661 cttcttcggt tttttttagt aatccgattt ttaccaacat ctttatgtgc gccatcgaac
      721 ccatgatgtc gtattttgcc agccgcaaat cgaattcccg gtcctttccc accgtaaagt
      781 tttctactga cttatcggca tcgaagcctt tgttccagat ttttgccata gtttctcttt
      841 ctaaatctat ccgctgattg acggaagagt tttttatttg atgatttttg tcatttcttc
      901 gataaaacgg atgtatccgg ctattccgct ttccagttcg ctgaccaaca cgaattcatc
      961 ggcctgatgg gagcgggccg attcgcccgg acccattttg atggccggac aggaaatacg
     1021 catccagtcc gaagtagtgg gggagacata tgttttcact cctaattgcc cgattgcttt
     1081 tatcaacagg tgatcggatg gcgtagccga acttttgttc gtgaggtttc gggcggagag
     1141 tttgcttttt acgttaaccc ggagaagttg caggatttcc ggattggtgt attggtcggt
     1201 tggacggata tccactacaa aagtacattt atccggaaca acgttatgtt gcgtaccggc
     1261 ggaaatctgt gtaacggtaa gtttcacttc gcccattgtc ggtgatatct tgtcaaactt
     1321 aatcgatctc atcgtctgaa tatcctccag ggcgatatac agcgcattca cgccctcgtc
     1381 tctcgccgca tgtccgctca caccggaagc tattccatcg ataaccagta gccctctctc
     1441 ggcaatagca gcctgcatcc cagtcggttc gcctataagg gcaaaatcaa tgtctggaag
     1501 ttcttttgcg agacgagtca tcccgtttgg ccccgaattt tcttcttcgc aggataaaac
     1561 caacataagg ttaaaaggca gttcagattc gtaaaaatgc aagaacgtct gcaacagaca
     1621 aactacgctt gccccagcat cgttgctgcc tagcccgaaa atgtgggtgt cggataaagg
     1681 cggattaaac ggatcgaaag tataggttgc agcaggcctt acggtatcga tatgcgaatt
     1741 gagcattagt gtttgcttcg ccatgttgtg gtgtggctga cgggcaataa tgttgttgcc
     1801 tgaccgttcc gtctctaccc ctctttcgga aagatagctg cacaaaaaat cactccgcac
     1861 tttttcttcg cctgaaaaag atttcagaga aaccaattct ttcagcaggt tggccgaacg
     1921 acggatatat tctttgttca tgggcatctt gacttttatc caattaacct tgtccctgtt
     1981 ttcttcagta aatttctcgc atgtaggatc acaacttcgg atacgccttc ctgtatggcg
     2041 ttaaatgaat tatcgagctt ggggatcatc ccgtcggcaa tcacgccttg ccctttcagc
     2101 tggataaatg cttctcggtt tatcaatggg atcaggctgt ttttgtcatt gatgtcccgc
     2161 aaaacacctt ctttttcgaa acagtaatac aacgtagtgc gatagtgctt cgacaacgct
     2221 acggcaagcg atgaggccac tgtgtcggca ttggtgttga gcagcgaacc gttgccgtca
     2281 tgggtgattg cgcaaaaaac cgggataacg tcgttttcaa tgaaagtgga gaggagtttc
     2341 ccgtccattt tccaggtgat ggggtcgcct acgaatccga agtcgatggg ttccgggttc
     2401 cggcgttccg atggaataca gttggcatca actcccgaaa gtccgatagc gttgcatccg
     2461 attttttgca gggcagcgac gatacttttg ttgatccatc cggcataaac catcgttacc
     2521 aattgcaatg tctcggcatc cgtaacccgt cggccttcaa ccattttagt cttgattccc
     2581 agtcgttcgg ccatttttga agccattact ccgccgccgt gaacgagtaa tttccttcct
     2641 ttcaactggt ggaagtcatt taggaaacca gttaaagctt cagggttatc gacaatgttt
     2701 ccgccaattt ttacgacaga aagtgagttg ttaaggtgga tggacatacg gtaatttttt
     2761 ttagtgttat agtgccagca agatttcttt caagaccgtt tgtgctgaaa ccacacggtt
     2821 ggctgcttcg ggaatgacaa tcgactgcgg actttcgatg acctcatcgg tcacgatcat
     2881 gttgcggcgt acgggaaggc agtgcataaa ataagcgttg ttggtaaggg ccattttggg
     2941 ggtatcaacc gtccaggatc tgtccgtact taggattttt c
//
LOCUS       DAOSDR010000384         2962 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrimonas sp. isolate AMR_MDS_1826
            NODE_11757_length_2962_cov_3.188166, whole genome shotgun sequence.
ACCESSION   DAOSDR010000384 DAOSDR010000000
VERSION     DAOSDR010000384.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863355
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrimonas sp. (wastewater metagenome)
  ORGANISM  Petrimonas sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Dysgonomonadaceae; Petrimonas.
REFERENCE   1  (bases 1 to 2962)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2962)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:10:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,720
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 35
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2962
                     /organism="Petrimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11757_length_2962_cov_3.188166"
                     /isolate="AMR_MDS_1826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2023866"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..690)
                     /locus_tag="PLI30_12270"
     CDS             complement(<1..690)
                     /locus_tag="PLI30_12270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009292084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HOI80364.1"
                     /translation="MLVTGADGFIGSHLVECLLNKGYQVRAFVFYNSFNSLGWLDTLP
                     KSELDEIDVFVGDVRDPNGVKKAIQGMDMVFHLAALIAIPFSYHSPDSYVDTNIKGTL
                     NVLQAARDTGCSRVLITSSSEVYGTARHVPIDESHPFQGQSPYSATKIGADRIAESFY
                     RSFNTPVTIVRPFNTYGPRQSARAVIPTIITQLLNGATEIKLGSTHPTRDFNYVKDTV
                     AGFLAIAEAEAT"
     gene            complement(945..1661)
                     /gene="pgl"
                     /locus_tag="PLI30_12275"
     CDS             complement(945..1661)
                     /gene="pgl"
                     /locus_tag="PLI30_12275"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: protein motif:HMM:TIGR01198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017057 - 6-phosphogluconolactonase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HOI80365.1"
                     /translation="MKKEIKVFSDTEKIAHEIANNLLALAKESNGKGIHIGLSGGSTP
                     KVIFRYLIEKYGKTLAYPNFHFWWGDDRCVPPHNDDSNYKWATDLWLKPIGITPEKIH
                     RVLGENDPEQEVFRYAGEIRKFIPFENGLPVFDYMLLGLGEDGHTASIFPCQMNLLTS
                     TKICEVATHPVSGQKRITLTGPVINHSRTIVFLATGENKAQRVRDIAVDQLPQYTASH
                     IGSSSGRVIWLVDSDAAKLL"
     gene            complement(1780..2169)
                     /locus_tag="PLI30_12280"
     CDS             complement(1780..2169)
                     /locus_tag="PLI30_12280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012606488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family EPS-associated transcriptional
                     regulator"
                     /protein_id="HOI80366.1"
                     /translation="MPIPKNPDGFQSEEILKVLRLLKDNPEMTQRELSSRLGLSLGKT
                     NFLMRAVIKRGLVKVQNFKNSQNKNAYLYYLTPSGMEEKARTTYFFPKRKMREYEQLE
                     EEIRQLRKEASDIGTVSPADFQNKDLL"
     gene            complement(2233..>2962)
                     /locus_tag="PLI30_12285"
     CDS             complement(2233..>2962)
                     /locus_tag="PLI30_12285"
                     /inference="COORDINATES: protein motif:HMM:NF025017.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOI80367.1"
                     /translation="NRGFAAANNQALAAMSGRYALLLNSDAVLAENAVPELFRFMEIC
                     PEAGMACGQLLNADGSNQNSIAPFPTLLTLAVNLSLLEYLFPKRYPSKRYRHDAPIEI
                     ESGVGACLILRRKALDQVGFFDERYFFFFEETDLAMSMRRAGWKIFHVPAARIYHYQG
                     QSIGRNENARIQFYRARYQFLQKWRSPVYCRSARIVILARLIVNWFSTMLAVLATLGL
                     NGPLLDRWRIYSRIIRWHFRPFSS"
BASE COUNT          707 a          766 c          762 g          727 t
ORIGIN      
        1 cgtggcttct gcttcggcga ttgccaggaa accggctacg gtgtccttaa cgtaattgaa
       61 atcgcgggtc gggtgagtgc tgcccagttt gatctccgtg gcgccgttca gcagctgggt
      121 aatgatcgtc ggaatgaccg cgcgggccga ttgacgcgga ccgtacgtgt tgaacggcct
      181 gacgatggtc accggggtat tgaaactgcg atagaagctc tctgctatgc ggtctgcacc
      241 gattttggtg gcggagtagg gcgactgtcc ctggaaggga tgcgactcat caatcggaac
      301 atgtcgcgct gttccataaa cttccgatga agatgtgatc agcacgcggc tgcaccctgt
      361 atcgcgcgcc gcctgaagca cattcaaggt acctttgata ttcgtatcga catagctgtc
      421 tggagagtga tagctgaacg gtatggcaat cagcgccgcc agatggaaca ccatgtccat
      481 cccttgtatg gctttcttca ccccattggg gtcgcgcaca tcgcctacga atacatcgat
      541 ctcatcaagc tcactctttg gaagcgtatc cagccacccc aggctgttga aagaattata
      601 gaaaacgaat gcccgaacct gataaccctt gttcagcaga cactcaacaa gatggctgcc
      661 gataaatccg tcagctccgg tgaccaacac tttttgtctc gaaggataca ctgtagtatt
      721 ggatcggtag tgtcccgtta aggtttcatg accaagaggt caacaccttt gggaaaagac
      781 caaagacgcc ataaagcaaa atatgggacc tacaatcggc aaccaaacga atctattcca
      841 ctcttaactg gacagtactg agatgaagct tataggcgat cagttcatta ctgtggccca
      901 ttgcgtggtt taccaaaaag tagccggcaa agattccctt gatttcaaag cagttttgcc
      961 gcgtcagaat cgaccagcca gattactcgt cctgaagacg atccgatgtg cgatgccgtg
     1021 tattgtggga gctgatcaac ggcaatatcc ctcactcgtt gcgctttatt ttcaccggtt
     1081 gccaggaaga cgatcgtccg ggaatgattt atgaccggcc cggtaagggt gatccgtttc
     1141 tggccactta cgggatgggt agccacctcg cagatctttg tggaggtcag caggttcatt
     1201 tggcagggga atatggaggc cgtatggcca tcttcgccta acccgagcaa catgtaatcg
     1261 aataccggca gcccgttttc gaaaggaatg aatttcctga tttcaccggc ataacgaaag
     1321 acctcctgtt caggatcgtt ttcacccaaa actcggtgaa ttttttctgg agtaatgcca
     1381 attggcttca gccacagatc ggtggcccat ttgtagttgc tgtcgtcgtt atgggggggc
     1441 acacatcggt catcacccca ccagaagtga aaatttgggt aggccagcgt tttgccatat
     1501 ttttcaatta aataccggaa aatcactttt ggcgtcgatc ctcccgacaa accaatatgt
     1561 atacccttcc catttgattc tttggccaat gccaataaat tattagctat ttcgtgggca
     1621 attttctctg tatcactgaa tactttgatc tcttttttca tctcctgttc tttgagtgac
     1681 cgttgaaaga tctatataca gctccgcccc ctggattaca gatcggcgtt cgaatccagc
     1741 aggcggcaag acactatcaa tcgccgtacc gttccagaat caaagaagat ctttgttctg
     1801 gaagtcggcc gggcttactg taccgatatc cgatgcctct ttgcgcaact ggcggatttc
     1861 ctcttccagt tgttcgtact ccctcatctt tcgcttcgga aagaaatagg tggttctcgc
     1921 cttttcctcc attccgctgg gtgtgaggta gtacaggtaa gcattcttgt tttgggaatt
     1981 cttgaaattc tgcaccttga caagaccgcg cttgatcact gccctcatca gaaaattggt
     2041 cttgccgagg ctgagtccga gacgggaaga gagctcccgc tgagtcattt cgggattgtc
     2101 cttcagcaga cggaggacct tgaggatctc ctcgctctgg aaaccgtcgg ggtttttggg
     2161 gataggcata atgatttcaa tcctgttcac gccatgaacg cgtagtgaac ctactccatc
     2221 agccgcatta agtcaagaag aaaaagggcg gaaatgccag cggatgatgc gggagtaaat
     2281 gcgccaccgg tccaggagcg gaccgttgag accgagagtg gcaagaaccg ccaacatcgt
     2341 ggaaaaccag ttcacgatca gccttgccag aatgacgatc cgggcggaac ggcagtagac
     2401 cggacttctc catttttgaa ggaattggta acgcgcgcgg tagaactgga tgcgtgcgtt
     2461 ctcattccgc ccaatgcttt gtccctgata atggtaaatg cgggcggccg gcacatggaa
     2521 gatcttccag cctgcccgtc gcatgctcat ggcgagatcc gtctcctcga aaaagaaaaa
     2581 gtacctctca tcgaagaaac cgacctggtc cagcgccttt cttctcaaga tcaggcatgc
     2641 accgacgccc gactcgattt cgatcggcgc atcgtgccgg tatcgtttgc tcggatatct
     2701 cttgggaaaa agatattcca gaagggaaag attcaccgcc agcgtcaaca aggtcggaaa
     2761 cggtgcgatg gagttctgat tcgaaccgtc ggcgttcaga agctggccgc aggccatacc
     2821 ggcctccggg cagatctcca tgaatcgaaa caattccggc accgcgtttt cggccaggac
     2881 cgcatcggaa ttgagaagca gggcataacg gcccgacatg gcggccagcg cctgattgtt
     2941 ggccgctgca aaaccccggt tc
//
LOCUS       DAOSDR010000385         2953 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrimonas sp. isolate AMR_MDS_1826
            NODE_11813_length_2953_cov_2.396825, whole genome shotgun sequence.
ACCESSION   DAOSDR010000385 DAOSDR010000000
VERSION     DAOSDR010000385.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863355
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrimonas sp. (wastewater metagenome)
  ORGANISM  Petrimonas sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Dysgonomonadaceae; Petrimonas.
REFERENCE   1  (bases 1 to 2953)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2953)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:10:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,720
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 35
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2953
                     /organism="Petrimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11813_length_2953_cov_2.396825"
                     /isolate="AMR_MDS_1826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2023866"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1887)
                     /locus_tag="PLI30_12290"
     CDS             complement(<1..1887)
                     /locus_tag="PLI30_12290"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HOI80368.1"
                     /translation="MENKIKSNQRAFKKCNRTLKIMKFSFLMLFFCFFTITAGNVFSQ
                     QKELSLNLRNVTLKKAISEIEKASDYVFLITDEVQLELNKKASIHANKESIHTILEAL
                     LKDTSLEFTVVERQVSVYKNNSSKKTEPAVEKIKQQKKTISGTITDVQGKPVIGANIL
                     EKGTTNGTVTDIDGNFTLQVDENAILHISYIGYLAQDVPTAEESNFKIILLEDVMALE
                     EVVVTGFGIEKRKKALGYSVTEVSGDDLTEAREINIADALAGKVAGVNVSNVATGASG
                     SSQILIRGNTSLSASNQPLYVIDGIPIDNSQLGVPGTWGGSDWGDGTSSLMNSSDIDN
                     ISILKGNAAAALYGSRASNGVILITSKKGDKRDGVGIEFSTNLVLEDMIDFTDYQKEY
                     GHGFNGAKPTSQSEALSYGLYSYGAKLDGSSVIQYDGVSRPYSYVGSNLKKFYRTGST
                     WTNTLTLTGGNENNAFRFTVSNLDNRGIVPENDVNRKNFSLSVNGLYWKKLSALAKVD
                     YIIEDVSHRSEISDSPGNVNYTLFSLPPSIDVTNFRGTTDKLGANEEGTELQFNSNVY
                     VNNPYWVAYQYKRSSERNRIIGSLALKYDFTDWLYLQGRLGRDWSHFFRTYLTPYGTG
                     YIPRG"
     gene            complement(2022..>2953)
                     /locus_tag="PLI30_12295"
     CDS             complement(2022..>2953)
                     /locus_tag="PLI30_12295"
                     /inference="COORDINATES: protein motif:HMM:NF016648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FecR domain-containing protein"
                     /protein_id="HOI80369.1"
                     /translation="EKRKHRLQVWLTATAAILVITILSTLFVRLNKDNGSAFLTENSS
                     IIGETLPKEEICLFSGGDKINIANQSDIQLQKNAKVLITNNTEAKKELVLNSVKMNKL
                     VVPFGKQSTLMLADGTKIYINSGTQVDFPSEFNGKTREIHVNGEVFIDVAEDTKKPFI
                     VHTDRMDIRVLGTSFNVSAYRDDMEKTVVLVKGKVSVKTEQQVTEMLPNEKVELTNGQ
                     ITKETVDVSEYISWTNGVLEFYETPVSEILKKVGRYYNVQFESSPDIPLSARTCSGKL
                     FLSNNLDSVMTSVSVLSSTNYHRENNIIYITKK"
BASE COUNT          763 a          634 c          548 g         1008 t
ORIGIN      
        1 gcctcggggt atgtaacctg ttccgtatgg agtcaggtat gttctgaaaa aatgagacca
       61 gtctcttcct aaccgaccct gaagatacaa ccaatcagta aaatcatatt tcaaagccaa
      121 agagcctatt attctgttcc tttcgctact tcttttgtat tgatatgcta cccaatatgg
      181 attatttaca tacacattac tgttaaactg caattcagtc ccctcttcat tagccccgag
      241 cttatcggtt gtccctctaa aattagtgac atctatactt gggggcagtg agaatagtgt
      301 atagttcaca ttccctggag agtctgaaat ttcacttcta tggcttacat cctcaataat
      361 ataatcaact tttgctaaag cacttaattt tttccaatac agtccgttaa cacttaagct
      421 aaagtttttt ctgttcacat cattttcagg aacaattcct ctgttatcaa ggttggagac
      481 agtaaaacga aaagcgttat tttcatttcc tccggttagt gtcagtgtat ttgtccatgt
      541 tgaaccagta cggtagaatt ttttcaaatt gctgccaacg tatgaatatg gacgcgatac
      601 gccatcatat tgtattacac tactaccatc aagttttgcc ccgtaactgt aaagtccata
      661 actcaacgcc tcggactgac ttgtaggttt tgccccgtta aagccatgac catattcttt
      721 ctggtaatct gtaaagtcaa tcatatcttc cagtacaagg ttagttgaga actcgattcc
      781 tactccatct cttttatcac ctttcttgga tgtgatcagg attactccgt ttgatgctcg
      841 tgaaccgtaa agagctgcag cggcatttcc tttcaatatg ctgatgttat caatatcact
      901 ggagttcatc aaactcgagg tgccatctcc ccagtcactg cctccccagg ttccaggaac
      961 acccaactga ctattgtcga taggaattcc atcaatgaca tacaacggtt ggttgctggc
     1021 cgagagagat gtattacccc tgataagaat ttgacttgag ccggaagctc ctgtagcaac
     1081 gttgcttaca tttacaccag ctacttttcc tgccagtgca tctgcaatgt tgatctccct
     1141 ggcttctgtc agatcatccc ctgatacttc cgtgactgaa tagccgagcg ctttttttct
     1201 cttttctatt ccgaaaccgg taactaccac ctcttcaagt gccataacgt cttcgaggag
     1261 aatgattttg aaattacttt cttctgctgt tggaacatcc tgcgctaaat aaccaatata
     1321 agaaatatga agtatcgcgt tttcatctac ctgcaacgtg aagttgccat ctatatcagt
     1381 aacagtgcca ttggtagtgc ctttttccag gatattagcc cctataacag gttttccttg
     1441 tacatcggta attgttccgg agatagtttt tttttgttgt ttaattttct caacagctgg
     1501 ttctgttttt tttgaagaat tgtttttata gacagaaacc tgccgttcca cgacggtaaa
     1561 ttccaatgac gtatctttca ggagtgcctc cagaatagta tgaatggatt ctttattcgc
     1621 atggatgctg gcttttttat tgagttcgag ttgtacctca tccgtaataa gaaaaacata
     1681 gtcacttgct ttttcaattt ccgaaatcgc tttttttaga gtaacattac gtagattcaa
     1741 agaaagttct ttttgctgcg aaaaaacatt tcctgcggta atggtgaaaa agcagaaaaa
     1801 gagcattaaa aatgagaact tcatgatttt tagggttctg ttgcactttt taaatgcacg
     1861 ttggttagat ttaattttat tttccataca tttgtaatga atttttagtt taggtaattt
     1921 attttctaag gattatctgt gaattaaatt tccacccgat cgggcgatgt gccaccgtcg
     1981 cccgatcttt ttgtttcttt ttttattttt tcataggcaa tctatttttt agtaatataa
     2041 ataatgttat tttcgcggtg atagttagtt gacgacaaaa cggagaccga ggtcatcacg
     2101 ctgtccaggt tattcgatag aaaaagcttt cccgaacagg tccgggcact gagcggaata
     2161 tccggactgc tttcgaactg cacgttgtaa tacctcccta ctttttttag tatttccgaa
     2221 acaggggttt cgtaaaattc cagtacgcca ttcgtccaac tgatgtattc agagacatcc
     2281 accgtttctt ttgttatctg accgtttgtc aattccactt tttcgttggg aagcatctct
     2341 gttacctgtt gttccgtttt tacggagacc tttcccttta ccagaacgac tgttttttcc
     2401 atatcgtcgc gataggccga aacattgaat gatgtgccga gtacacggat atccatccgg
     2461 tcggtatgaa cgataaacgg tttttttgta tcttctgcaa catcaatgaa aacttcgccg
     2521 ttaacatgaa tttctcttgt ttttccatta aattcggacg gaaaatcgac ctgtgtcccg
     2581 gagtttatat agattttcgt tccgtcggcc agcataagcg ttgattgctt gccgaaaggg
     2641 accaccagct tattcatttt cacggaattc aataccaact ctttttttgc ttcagtattg
     2701 tttgtaatca gaactttagc attcttttgc agctggatgt ccgattggtt ggcaatattg
     2761 attttatctc cacctgagaa gagacatatt tcttctttag gcagagtctc tcctataatg
     2821 ctgctattct cggtcaaaaa agccgatcca ttgtctttat tcaaccgaac aaacaaggtg
     2881 gacaggattg tgatgacgag gatagctgca gttgccgtca accagacttg cagtcggtgc
     2941 ttccgctttt cgt
//
LOCUS       DAOSDR010000386         2944 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrimonas sp. isolate AMR_MDS_1826
            NODE_11862_length_2944_cov_2.387331, whole genome shotgun sequence.
ACCESSION   DAOSDR010000386 DAOSDR010000000
VERSION     DAOSDR010000386.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863355
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrimonas sp. (wastewater metagenome)
  ORGANISM  Petrimonas sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Dysgonomonadaceae; Petrimonas.
REFERENCE   1  (bases 1 to 2944)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2944)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:10:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,720
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 35
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2944
                     /organism="Petrimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11862_length_2944_cov_2.387331"
                     /isolate="AMR_MDS_1826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2023866"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1108
                     /locus_tag="PLI30_12300"
     CDS             <1..1108
                     /locus_tag="PLI30_12300"
                     /inference="COORDINATES: protein motif:HMM:NF014717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HOI80370.1"
                     /translation="TVQFVVAFALIIATLTANRQVSLVKQTGNRYRNLIEISGVMEIQ
                     ELQKLTSRLKNIPGVSGVSIGNLPVMNSWIMHGTLRKETGEEIETAVLQLSGDEELLD
                     ILNLRQLSGDDWRSSFQINPYSVLVNKTFVDILGKRETELTNEPLSKYFDVGDSVSVI
                     AGIVEDFHFNSLEEKTTSFVIGRYAYDPRYTSTLRVRLDGKNDSETFSAIKSAWWQTF
                     PDDGFTYIDTYNEFIKLNIKIFEMSRLLDMYSLISILLTCFGLFGITFYAVRQRTKEI
                     GIRKINGAKTPQLLWLLMKPMFVWLAVGFVVAAPLAWWLMERWLQQFVYRIDVSVSSF
                     LLALLLVAVITFLTVGWHVWRMARSNPVESLKAE"
     gene            1159..>2944
                     /locus_tag="PLI30_12305"
     CDS             1159..>2944
                     /locus_tag="PLI30_12305"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOI80371.1"
                     /translation="MKLLALPFRVLGRFRLYSIVNMLGLAISLASVIVIARYINQETT
                     VNHFASDLKHTYILSIENPEDGRKRFGGATVRAEDNIPFPPLLNNPSVKNTTTFFAYE
                     EDFIVSGNHRINVKAIAADSNFFKILPFPIRYGSHKMIDPSDAIITENFAQKLFGKEN
                     PIGKKFTHSSGDELKVVGVTGKPSSKSFLAFDILLDKDQRQFMAIQAFTLIKLHTGAD
                     VRKLNDSNRQLDESRSVAPNPVRLQLFPLKDFYFDRTHEVWKITRNNDLSVFVKGNRN
                     AVKILLVVGILILLVGLFNFINIYTVITLKRGKEFGVKKIFGAGKGQIFWQIYGENLL
                     MTLIAVFLAWFFIEIAQSYLSTRLNFEVIPNPVFSILFSVLILIFLPLITSLHPYLRY
                     HYSKPIASLQSVYVRGVSLASRKTFLFLQYVITFGLIVIALFFMKQLNYMLAVNPGYN
                     TKDVIMARMMHRDMNAFRSSDMEAAFRKISENATLVRDRMNKSPLFSDWEFGLPVYNL
                     EAKVPMKRSDRSNYQPVSSVGMSPQYMKMFGFQLKEGRLWDSTDVNGEFKYIINESAA
                     KLFEITDIRTVKLESQYKREGETAPIHQV"
BASE COUNT          857 a          610 c          653 g          824 t
ORIGIN      
        1 taccgtgcag tttgttgttg ctttcgcttt gatcatcgcc acccttacgg caaaccggca
       61 ggtaagcctg gtgaaacaaa ccggcaaccg gtaccggaac ctgatagaga tatctggggt
      121 gatggagatt caggaacttc aaaaactgac atcccggtta aaaaatattc ccggcgtatc
      181 cggtgtttcc ataggaaacc tgcccgtgat gaactcatgg attatgcacg gcacgttacg
      241 aaaagaaacc ggggaagaaa ttgaaacggc ggtgctgcaa ctctcgggag atgaagaatt
      301 attagacata cttaatctcc ggcaactttc gggagacgat tggaggtcat cattccaaat
      361 caatccatat tcggtactgg tgaacaagac gtttgtcgat atattgggca aacgggaaac
      421 ggagttaacg aacgagccct tatccaagta tttcgatgtg ggagattcgg tatccgttat
      481 cgccggaata gtggaagatt ttcacttcaa ttccttagaa gaaaaaacaa cgtcgtttgt
      541 gatagggcga tatgcttatg atccgagata caccagcaca ttgcgtgtca ggctcgacgg
      601 caagaatgat tccgaaacct tttccgccat taaatcggca tggtggcaaa cctttcccga
      661 tgacgggttc acttacatcg acacctataa tgaatttatc aaactcaaca ttaaaatctt
      721 cgagatgtcg cgcctgctcg acatgtactc gctgatcagc atcctgctta cctgtttcgg
      781 gttgttcggc atcacgtttt acgccgtgcg gcagcgaacc aaagaaatcg gaatccgaaa
      841 aataaacggg gcgaaaacgc cgcaactctt atggctgctg atgaaaccga tgttcgtgtg
      901 gttggcggtg ggatttgtgg ttgccgcgcc gttggcgtgg tggctgatgg aacggtggct
      961 gcagcagttt gtttaccgga tagatgtatc ggtaagctcg tttttacttg cattgttatt
     1021 agtcgccgtt atcacatttt taaccgttgg ctggcacgtg tggcgcatgg cgcggagcaa
     1081 tccggtggaa agtttgaagg cagagtaaag tcaaagtcta ttaaataaaa ctatatttat
     1141 taaaatataa gataaataat gaaattacta gcattgccgt ttagagtttt aggccgattc
     1201 agactgtatt caattgtcaa catgttggga ttggcaataa gtctggcgtc tgttattgtt
     1261 attgcgaggt atatcaatca ggaaactacc gtaaatcatt ttgcttcgga tctgaaacac
     1321 acttacatct tatcgataga gaatcccgaa gacgggcgaa aacgttttgg tggagctact
     1381 gtacgagccg aagacaacat cccctttcct cccttgttga ataatccttc ggtaaaaaat
     1441 acaactacat tttttgcata cgaagaagac tttatcgttt caggcaacca ccgtatcaat
     1501 gtaaaagcaa tagctgccga cagcaatttc tttaaaattc taccgtttcc aatacgttac
     1561 ggaagccata aaatgattga tcccagcgat gcaatcatta ccgaaaattt cgcacaaaag
     1621 cttttcggga aagaaaatcc aatagggaaa aaattcaccc actcttcggg cgacgaacta
     1681 aaagtggttg gagttaccgg aaagccttct tccaaatcgt tcctcgcttt tgatattctc
     1741 cttgacaagg atcagcgcca atttatggct atacaagcgt ttaccctgat caaattacac
     1801 acaggagccg atgtgaggaa attaaacgac agcaatagac agttagacga gtcgagatct
     1861 gtcgctccca atcctgtccg tcttcagtta tttcccttga aagattttta cttcgaccgt
     1921 acacacgagg tatggaaaat aacacgtaac aatgatcttt cggtatttgt gaaggggaac
     1981 agaaatgccg tgaaaatact tctcgttgta ggtatactca tcttgttggt ggggcttttt
     2041 aacttcatca atatctatac ggtcatcaca ttgaaacgcg gaaaggagtt tggtgtaaaa
     2101 aaaatattcg gagccggtaa aggacaaata ttttggcaaa tctatggtga aaatctcctg
     2161 atgacgctaa ttgcggtctt tttagcctgg ttcttcatcg agatagctca atcgtattta
     2221 tcaacccggc ttaactttga ggtgatacca aaccctgtgt tcagtatctt attttcagtt
     2281 ttaatcctga tttttctgcc tttaataacc tcactacatc cttatctcag gtatcattat
     2341 tcaaagccca tcgcgtcgct ccaatctgta tatgtgcgag gggtttcact ggcctcacgc
     2401 aaaacgtttc tgtttctgca atatgtcatc actttcgggt tgatagttat tgctctcttt
     2461 tttatgaaac aattgaatta tatgctagcc gtcaatcccg gatacaacac caaagatgta
     2521 attatggcaa gaatgatgca ccgtgatatg aatgctttca gaagctccga catggaagca
     2581 gcattccgca aaataagcga aaatgcgact cttgttaggg acaggatgaa caaatcgcct
     2641 ttattttcag actgggagtt cgggcttcct gtgtataatc tggaagcgaa agttccgatg
     2701 aaacgttccg acagaagtaa ttatcaaccc gtaagttctg tcgggatgtc gcctcaatac
     2761 atgaagatgt tcggttttca gttaaaagaa ggccgcttat gggattcaac cgatgtgaac
     2821 ggtgaattta aatacatcat caatgaatcg gctgcgaaat tatttgaaat aactgatatt
     2881 cgaacggtaa aactcgaatc gcaatataaa agagaaggag aaacagcgcc gattcatcag
     2941 gtag
//
LOCUS       DAOSDR010000387         2932 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrimonas sp. isolate AMR_MDS_1826
            NODE_11930_length_2932_cov_3.400417, whole genome shotgun sequence.
ACCESSION   DAOSDR010000387 DAOSDR010000000
VERSION     DAOSDR010000387.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863355
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrimonas sp. (wastewater metagenome)
  ORGANISM  Petrimonas sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Dysgonomonadaceae; Petrimonas.
REFERENCE   1  (bases 1 to 2932)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2932)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:10:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,720
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 35
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2932
                     /organism="Petrimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11930_length_2932_cov_3.400417"
                     /isolate="AMR_MDS_1826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2023866"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(77..1303)
                     /locus_tag="PLI30_12310"
     CDS             complement(77..1303)
                     /locus_tag="PLI30_12310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006841520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HOI80372.1"
                     /translation="MRRLIVITIVISTLIASCTLQDTPKLKEGVWRGELAVQDKWTPF
                     IFEVKTMGNDSVAVVLRNGDERVELSNVTFSNDSVTIPIEAYDAFIRAKLNGKNLEGK
                     FLKNYIENDQGVPFKAEFNQTDRFPVVSNPSEIRIDGKWDIHFVDEKSDTTRNVGVFK
                     TDNNTVTGSVLTNAGDLRFLEGNYTDTGVQLSAFGGLSPYLIEISFRDNDNFDGQFYT
                     TRGITKLIGVRNDQASLADPYTLTNMKPGYESLHFSLPNLDGELVSVDDNRYKEKVVI
                     VSILGSWCPNCLDEMEYLSPWYTENKNRGVEIIGLAFERKDDLNYAKSTLSRLINKYY
                     VDYEILFAGQLGDATVQKVLPEIDKLSSYPTTFFIDKKGKVRKIHTGFTGPATGLFYE
                     EFKKDFNSLIDSLLLE"
     gene            complement(1315..2919)
                     /locus_tag="PLI30_12315"
     CDS             complement(1315..2919)
                     /locus_tag="PLI30_12315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019539574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SusD/RagB family nutrient-binding outer membrane
                     lipoprotein"
                     /protein_id="HOI80373.1"
                     /translation="MKRASKLFTPKNTIKSESHTIPLKVTKIYSVITGLILIVLTSCI
                     NEDLNIDPNRPTFVPTTSLIVTAEKKLVDNLRNESVSLRGSALFVQQLSQNTYTSQSR
                     YDIPFGYSAEYWSELYKVLNNLQEIIDLNTDPSTKDIVTASGAGRNANQIAISRILKS
                     YAYYALTDVFGNIPYNSYGNNDPDFQALQQNPDNITPKYASQEKIYPDILNELKQAAD
                     TLLKYSNEVTFGKSDIIYAGNNGKWAKFANSLRLRFSTRLKAKNPDLYQSHFSDALNK
                     GVFTSNDDNAVYKYSTVAPNEAPLYRATVTANRKDFAISHIFVNLLKGENSQLPVADP
                     RLSVYALKNSAGNFVGLPYGMTEAQAGKFSAQNISLPGALYNAANYGEVLQEYSEVAF
                     LISEFNEWSQSDYEKAVKASLEKWGIPSADIQNYVNALPAANQQNVLNQKYIALFTQF
                     LESWSEYRRTGYPNFLVKRNDVVFNGIVEGENVSYTFNPLFGDGGVPSRLYYPVKEQT
                     VNKESYQDAIASQGGDVIETKLWIFK"
BASE COUNT          846 a          614 c          532 g          940 t
ORIGIN      
        1 gaggtcggaa aacgaaagta ggaagaaaac tacttcgttt tcctcctact cgatttctat
       61 aaaaacaact ctgctattat tccaaaagta aactatcgat tagcgaattg aaatctttct
      121 tgaattcttc gtaaaataaa ccggtggccg gcccggtaaa tccggtatgt attttacgga
      181 cttttccttt tttgtcgata aaaaaagtcg tgggataact ggagagttta tcaatctcgg
      241 gaagaacttt ttgaaccgtg gcatccccca attgtccggc gaagagaatt tcataatcaa
      301 catagtattt atttatcaat cgggagagcg tgcttttcgc gtaattcaaa tcatctttac
      361 gctcaaatgc aagtccgatg atttccactc cacggttttt attttccgta taccagggag
      421 acaagtattc catttcatca agacagtttg ggcaccagct acctaaaata gaaacaataa
      481 ccactttctc tttatagcgg ttatcatcta cagaaaccag ttctccatcg agatttggaa
      541 gtgaaaaatg taatgattca tagccaggct tcatgttagt aagcgtatag gggtctgcta
      601 aagacgcttg gtcatttctt actccaatca atttcgtaat accgcgtgtt gtataaaact
      661 ggccatcaaa attatcgtta tcacgaaaag aaatttcaat caaataaggg cttaaaccgc
      721 caaaagccga aagttggacg ccagtatcgg tataattccc ttccagaaaa cgcaagtctc
      781 ctgcgtttgt cagaactgaa ccggttactg tgttattatc ggtcttgaat acaccaacat
      841 tgcgtgtggt atcgcttttc tcgtcaacaa aatggatatc ccatttacca tcaattctaa
      901 tttcagacgg attggagaca accgggaatc tgtctgtctg attaaactct gctttaaaag
      961 ggacaccctg atcattttca atgtaatttt tcagaaattt tccttcgaga ttctttccat
     1021 ttagttttgc cctgataaaa gcgtcgtatg cctcaatcgg aatggttact gaatcgttgg
     1081 agaaagtaac attagaaagt tcaactcttt catccccatt cctaagaact accgcgacag
     1141 agtcattccc catcgttttt acttcgaaaa taaacggcgt ccatttatcc tgtacagcca
     1201 attcacctct ccagactccc tcctttaatt ttggagtgtc ttgtaatgtg cacgatgcaa
     1261 tcaacgtgct gattacaatt gttattacta ttaatcttcg cataatcttc agttttactt
     1321 aaatatccat aatttggttt caatcacatc tccgccttgt gaagcaatgg cgtcctgata
     1381 actttcttta tttacagttt gttcctttac cgggtaatac aatcgcgaag gcacaccccc
     1441 atctccaaac aacggattaa aagtgtaaga aacattttca ccttcgacga ttccgttgaa
     1501 tactacgtca tttcttttaa ccagaaaatt cggatacccc gtacggcgat attcgctcca
     1561 actttccaaa aattgagtaa acagtgctat atacttctgg tttaatacgt tttgctgatt
     1621 tgccgcaggt aatgcattta cataattttg gatatctgcc gacggtattc cccatttttc
     1681 caacgatgcc ttaaccgctt tctcatagtc ggattgactc cattcattaa attctgaaat
     1741 cagaaaagca acttcgctgt attcctgcaa aacttccccg tagtttgctg cgttgtacaa
     1801 cgcgcccggc aaacttatgt tttgagccga gaatttacct gcctgggctt cagtcattcc
     1861 gtaaggtagc cctacaaaat taccggcact gttttttagt gcataaacgc ttaatcgcgg
     1921 atcagctact ggcagttggg aattttcccc tttcaacaaa ttgacaaaaa tgtgagaaat
     1981 agcaaaatct ttccgatttg cggtaacggt tgctctatat aacggagctt cgttcggagc
     2041 gacagtggag tatttataaa cggcgttatc gtcattcgag gtgaacaccc ccttgttcaa
     2101 cgcatcggaa aaatgcgact gatacagatc cggattttta gcctttagcc gtgtagagaa
     2161 acgaagacga agcgaattcg caaattttgc ccacttacca ttatttccgg catatattat
     2221 atctgattta ccaaaagtta cttcatttga atacttgagt aatgtgtcgg cggcttgttt
     2281 taattcgttc aagatatccg gataaatttt ttcctgagaa gcatatttgg gcgtaatatt
     2341 atcgggattt tgttgcaacg cctgaaagtc ggggtcgtta tttccataag aattgtatgg
     2401 aatatttcca aaaacatcgg ttaaggcata atatgcatac gatttcagga tgcggctaat
     2461 agcaatttga ttagcgtttc gtccggctcc gctggcagtt acaatatctt tcgtagatgg
     2521 atcggtgttc aaatctataa tctcctgtaa attattcagt actttataca attcactcca
     2581 atattcggcc gaataaccaa aaggaatatc gtatcttgat tgacttgtat aggtgttttg
     2641 acttaattgc tgaacaaaca gtgccgaacc acgcaacgaa acactttcgt ttcgaagatt
     2701 atccactaat tttttttctg cagttacaat caacgatgtt gttggcacaa aagtaggacg
     2761 attgggatcg atgtttaaat cctcgttgat acacgacgtt aaaacaatca agatcaaccc
     2821 tgttataaca gaatatattt ttgttacttt caaaggtatc gtatgtgact cacttttaat
     2881 cgtgttcttg ggtgtaaata attttgatgc tcgtttcata tcaatagtta tt
//
LOCUS       DAOSDR010000388         2931 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrimonas sp. isolate AMR_MDS_1826
            NODE_11933_length_2931_cov_4.310153, whole genome shotgun sequence.
ACCESSION   DAOSDR010000388 DAOSDR010000000
VERSION     DAOSDR010000388.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863355
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrimonas sp. (wastewater metagenome)
  ORGANISM  Petrimonas sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Dysgonomonadaceae; Petrimonas.
REFERENCE   1  (bases 1 to 2931)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2931)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:10:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,720
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 35
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2931
                     /organism="Petrimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11933_length_2931_cov_4.310153"
                     /isolate="AMR_MDS_1826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2023866"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..416)
                     /locus_tag="PLI30_12320"
     CDS             complement(<1..416)
                     /locus_tag="PLI30_12320"
                     /inference="COORDINATES: protein motif:HMM:NF016296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HOI80374.1"
                     /translation="MFPLKAFVAFLIYLLLVQFFRVRLAEREASPQPVDEDTEEDLLK
                     PGDIGTEEIQPLERIVIKSGQKINMIQVSEIVYFRAEGDYVRIFTDSDKFLKEETMKY
                     FQLHLPEKQFVRVHRSYLVNTVKILRIELYEKQNQLL"
     gene            complement(717..1487)
                     /locus_tag="PLI30_12325"
     CDS             complement(717..1487)
                     /locus_tag="PLI30_12325"
                     /inference="COORDINATES: protein motif:HMM:NF021443.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LiaF-related protein"
                     /protein_id="HOI80375.1"
                     /translation="MENNEQQMNFSGRSKGGLFGFLLILAGTLFLAFNFGWIDPALRS
                     VVFSWPVLFVVFAIIALFKREYLALFFWLALAAFFLLPRIAHLYPDALPGINEGFTAT
                     FWPVLLIVVGAGVILKILFGKKACCHRHGHIQRPLNVDKMEGTDGIYVREVVFGGIED
                     IFLEPVFRGGQIEVVFGGVELDLRRASLPEGDTYLQVEAVFGGIKLYLPDDWVVVPKI
                     STVLGGVDNKHFSKSANHDTSRRLLISGEIVFGGCEIR"
     gene            1768..2349
                     /locus_tag="PLI30_12330"
     CDS             1768..2349
                     /locus_tag="PLI30_12330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI80376.1"
                     /translation="MKQRTSFTTFAVVFSFLVFSGFLVFSGCTGSSQKGTLTVDNLLS
                     NPGEYVGQPVTVVGLATHVCAKSGMKLFLQGSTDAQSIRAESNSTLGKFNETCVDNKV
                     VVKGTLVEERITEADLQKMEKEIVENTTVTHGEGGEGCETEQKAEGVAVGSSEMDRVN
                     EFRKRLTERKTSEGKDYLSFYHIATESYSIVKK"
     gene            2346..>2931
                     /locus_tag="PLI30_12335"
     CDS             2346..>2931
                     /locus_tag="PLI30_12335"
                     /inference="COORDINATES: protein motif:HMM:NF020922.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2461 domain-containing protein"
                     /protein_id="HOI80377.1"
                     /translation="MKTVPSFSGFTPETIQFLVDLKENNFRQWFEDHRELYEIELLQP
                     FRALVNTLSPAMHNIDSLFEFRTHKVLSRIYRDIRFSKNKDPYNACLWMSFQRMGTSW
                     ENFPGYFMELNTEHVMLGMGLFAPKRKVMDVFREKIEDEPDTFRELVQKDVFGRGFSV
                     EGESYKKPVHNTLSDFFQPWMNRKAVYVMKTLPVS"
BASE COUNT          939 a          673 c          602 g          717 t
ORIGIN      
        1 agcagttgat tttgtttctc gtacaattcg atgcgcaata tttttaccgt gttgaccaaa
       61 taagaacgat gcacccttac aaactgcttc tcaggcaaat gaagttggaa atacttcatg
      121 gtttcttctt tcaaaaactt gtcggaatcg gtaaaaatac gcacataatc gccttcggcc
      181 cggaaataaa caatttctga aacctgaatc atgtttatct tttgtcccga tttgataaca
      241 atacgttcga gcggttggat ttcttctgtg ccaatatcgc ctggtttcaa caaatcctct
      301 tccgtatcct catctaccgg ctggggagag gcctcccttt cagcaagtct taccctgaaa
      361 aactgtacca acaggagata tatgagaaaa gcaacgaaag ctttcagcgg aaacataacc
      421 taaacccgtc caaatggtta tggctaagaa accaagcgca atgtaattga taaaacgctg
      481 ataaatttcc aatgtttcaa aattcccgta cttgaccact ttccagagca aaaaacttac
      541 acctgtaaaa aacaacgcat tcatcatccc gtccaacatc aattggggga aaggaaacgt
      601 tacaagcgaa cggaatgcca acgcctgcat aaatgcatag acgacgcaca ggacagatat
      661 cgtcaatata cgcgataaat tattcggaag aaaaaaattc attttactaa aactacttac
      721 cgaatttcac aaccaccaaa aactatctcg ccactgataa gcaaccggcg agaagtatcg
      781 tgattcgctg attttgagaa atgtttatta tccactcccc ccaaaacggt acttatctta
      841 ggcacaacca cccaatcatc gggcaggtac aattttatgc cgccaaatac ggcttcaacc
      901 tgaaggtacg tatcaccttc gggcagtgat gcccggcgca gatcgagctc tactccccca
      961 aagacaactt caatttgtcc tccccggaac acaggttcca gaaaaatatc ttcgataccg
     1021 ccgaaaacaa cttcccgtac atagataccg tctgtgcctt ccattttatc aacattcaaa
     1081 ggccgttgta tatgaccatg acgatggcaa caggcttttt ttccgaaaag tatttttaat
     1141 ataactcccg ctccgactac tataagcaga acaggccaga aggttgcggt aaaaccttcg
     1201 ttaatacccg gcagcgcatc aggatataaa tgagcgatac ggggcaatag gaaaaaagct
     1261 gccagggcca accagaaaaa cagggccaga tactctctct taaaaagggc aataattgca
     1321 aaaacgacaa ataaaacagg ccaggaaaac acgacggaac gtaacgccgg atcgatccag
     1381 ccaaaattaa aagcgagaaa caacgttccc gctaaaatca gtagaaaacc gaaaagacca
     1441 cctttcgatc tgcccgagaa attcatttgt tgttcattat tttccataag atatattgtt
     1501 taaaactttt tttacacaaa gatacataca aagtgaagca tccggcaaga caaaatcgat
     1561 gaaacgaggt atttttcccg acgaatgaag taaaataccg gatgaatctt ctgtggaaag
     1621 acacaaagtc agaaatcttt aacacattca aactgaaatt gaagaaaaac aggagtggcg
     1681 catgactgaa aatatttttc ctgatttttc cgtttctgcc taaaaacaga tacctttgaa
     1741 gtttaatcat caattaaatc aatcaagatg aaacagagaa cctcatttac aacttttgct
     1801 gtcgttttct cattcctggt attttcggga tttctggtat tttcgggctg caccggtagt
     1861 tcacaaaaag gaacgttaac ggttgacaac ctgttgtcga atcccgggga atatgttgga
     1921 caacctgtca cggttgtagg ccttgccacg cacgtatgtg ccaaaagcgg catgaaactt
     1981 tttttacaag gcagtaccga cgcgcagagc attcgtgcag agtcgaacag tacactggga
     2041 aaattcaacg aaacgtgcgt tgacaacaag gtggtggtaa aaggcacatt ggtggaagag
     2101 aggatcacgg aagccgatct tcagaaaatg gaaaaagaaa ttgtcgaaaa cacaaccgtt
     2161 actcacggtg aaggcggtga aggttgcgaa accgagcaaa aagccgaagg tgtagcagtg
     2221 ggcagctctg aaatggatcg cgtgaacgaa ttccgtaaac gattaacaga acgcaaaact
     2281 tcagaaggaa aagattacct gtcgttctat cacatcgcca ccgaatcgta cagcatcgtc
     2341 aaaaaatgaa aaccgtgccg tcgttttcgg gattcacacc cgaaaccata cagtttctgg
     2401 tcgatctcaa agagaacaac tttcgccaat ggttcgaaga tcaccgtgag ctttacgaga
     2461 ttgaactgct acaacccttc cgggcattgg taaacacgct ctcgccggcc atgcacaaca
     2521 tagactcctt gtttgaattc cgcacgcaca aggtattatc gcgtatttac cgcgacatcc
     2581 gtttctcaaa aaacaaagat ccttacaacg cctgcctgtg gatgagtttt caacgcatgg
     2641 gcacatcgtg ggaaaatttt ccgggttatt ttatggagct taacacggag catgtcatgc
     2701 tgggtatggg gctctttgca cccaagcgca aagtgatgga tgttttccgg gagaagattg
     2761 aagacgaacc cgacactttc cgggaactgg ttcagaaaga tgtgtttgga cgaggttttt
     2821 cggttgaagg cgaaagttac aagaaacctg tccacaacac cctgtccgac ttttttcagc
     2881 cctggatgaa ccggaaagct gtttacgtga tgaagacgct tcccgtttcg g
//
LOCUS       DAOSDR010000389         2928 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrimonas sp. isolate AMR_MDS_1826
            NODE_11948_length_2928_cov_3.821441, whole genome shotgun sequence.
ACCESSION   DAOSDR010000389 DAOSDR010000000
VERSION     DAOSDR010000389.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863355
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrimonas sp. (wastewater metagenome)
  ORGANISM  Petrimonas sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Dysgonomonadaceae; Petrimonas.
REFERENCE   1  (bases 1 to 2928)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2928)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:10:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,720
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 35
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2928
                     /organism="Petrimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11948_length_2928_cov_3.821441"
                     /isolate="AMR_MDS_1826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2023866"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1166)
                     /locus_tag="PLI30_12340"
     CDS             complement(<1..1166)
                     /locus_tag="PLI30_12340"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004306525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase"
                     /protein_id="HOI80378.1"
                     /translation="MATKSFHYQDPFPMSKDTTEYYLLTKDHVSVSEFEGKEVLKISQ
                     EGLTLMAQTAFRDVEFLLRPEHQEQVAKILTDPEASDNDKYVALTFLRNSEISAKGIL
                     PFCQDTGTAIIMGKKGQQVWTEGNDEEALSRGVYNTFINENLRYSQNAPLNMYDEVNT
                     GTNLPAQIDLYAVQGNEYKFLCVAKGGGSANKTYLYQETKALLTPEKLEAFLTEKMKS
                     LGTAACPPYHLAFVIGGTSAEANLKTVKLASTKYYDNLPTEGNEFGRAFRDIEMEEKL
                     KIASEKLGLGAQFGGKYFAHDVRVIRLPRHGASCPVGMGVSCSADRNIKAKINKEGIW
                     IEKMEDNPTRLIPESMREAGEGGGVKINLNRPMPEILAELSKYPVATRLSLNGT"
     gene            1371..>2928
                     /gene="feoB"
                     /locus_tag="PLI30_12345"
     CDS             1371..>2928
                     /gene="feoB"
                     /locus_tag="PLI30_12345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009017843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HOI80379.1"
                     /translation="MRLSELQTGDKAFIVKVNGSGAFRKRILEMGFVRGQEVRSILNA
                     PLKDPIKYGIMEYEVSLRRSEAVLIEISKLKEDVVYAVDNDKTDEPENEVEETDFRDP
                     PLSDESDLKKENNNVIRVALVGNPNAGKTSIFNLASGAHEHVGNYSGVTVDSKEGTLR
                     HNGYKFILTDLPGTYSLSAYSPEELYVRSYIFEEKPDVIVNVVSASALERNLYLTTEL
                     IELEKPMVVALNMYDELEKSGRLFKHQTLSEMLNVPIVPTVGKKGFGIPELLENVISI
                     YESGNNSHNVKVPYGRVLEKSIGFMCRDLLSNGFSTLGMPKRYVGIKLLEGDKEVENA
                     IREHDKGKELLARRNKEREYIERLLKEDPESAFTNARYGFIAGALKETLSEKTKFEDK
                     TTVLDAVLTNKYLGLPLFFVFLWIMFEATFRLGAYPMEWIEWIVAQAGNLIRVNMTEG
                     PLKDLLVDGVIGGVGGVIVFLPNIVILYAFIAFMEDSGYMARAAFIMDKLMHNIGLHG
                     KSFIPLIMGFG"
BASE COUNT          745 a          600 c          747 g          836 t
ORIGIN      
        1 attgttccgt ttagcgaaag ccgggtagca accggatatt tggataattc cgccaggatt
       61 tccggcatcg ggcggttgag gttgatcttc acgccgccgc cttcaccggc ttcacgcatc
      121 gactcgggga taaggcgggt tgggttgtct tccattttct caatccagat accttctttg
      181 ttgatttttg ctttgatgtt gcggtcggcc gagcaggaca ctcccattcc tacggggcaa
      241 gaggctccgt gacggggcag gcggataacg cgcacgtcgt gtgcaaaata ttttccaccg
      301 aattgagcgc ccagtcccag cttttcagac gcgatcttca gtttttcttc catttcaata
      361 tcgcggaacg cccggccaaa ttcattgcct tcggtgggga gattgtcgta atattttgtg
      421 gatgccagtt ttacagtttt caagttggcc tcggcagagg tgccgccgat gacaaatgct
      481 aaatgatagg gcggacaggc tgcagttccc agcgatttca ttttttccgt caggaacgct
      541 tccagttttt caggggtgag caacgctttg gtttcctgat acaggtatgt tttattggct
      601 gatccgccgc ccttggcaac acaaaggaat ttgtactcat tcccttgaac cgcgtacagg
      661 tcgatttgag cagggaggtt tgtccccgta ttcacttcgt catacatgtt gagcggcgca
      721 ttctgcgaat agcgtaggtt ttcgtttata aacgtgttgt aaacaccacg cgagagggcc
      781 tcctcatcgt ttccttccgt ccatacctgt tgcccttttt tgcccataat aattgctgtt
      841 cccgtgtcct ggcagaaggg aagaattcct ttggccgata tttccgaatt gcgcaggaaa
      901 gtgagcgcca cgtatttgtc gttatcgctc gcttcggggt cggtaagaat tttggctacc
      961 tgttcctgat gctcgggacg cagcagaaat tccacatcgc gaaaagccgt ctgtgccatc
     1021 agtgtaagtc cttcctgaga aattttcaat acctctttcc cttcaaattc cgaaaccgat
     1081 acgtgatctt tggtcagtag gtagtattct gtggtgtctt tcgacattgg gaaagggtct
     1141 tgataatgaa acgattttgt tgccatataa tgatgttttg taaatgtttt ttcgaacagg
     1201 tacaaaatta caaagaaaaa atgacttttt agcaggcagt agagtagaca acctttcgat
     1261 tgctcagttg tttgtgctat ttaaattcat tataaataat atatcttaaa agctcgtttt
     1321 ttaggcaaaa aatatgttcc tttgtatccg gaaatgagat tgtgctattt atgcgacttt
     1381 cagaactaca gaccggcgat aaggcattca ttgtaaaagt aaatggatcg ggggcatttc
     1441 ggaaacggat tctcgaaatg ggcttcgtgc gcggacaaga ggtgaggtcg atactgaacg
     1501 ccccgttgaa agaccctatc aaatacggca tcatggagta cgaagtttcg ttgcggcgca
     1561 gtgaagccgt attgattgaa atttcgaaat tgaaagaaga tgtggtgtat gcggtggaca
     1621 atgacaaaac agatgagcct gaaaacgagg ttgaagagac ggactttcgc gatccaccac
     1681 tttctgacga atccgattta aagaaagaaa ataataacgt tattcgtgtt gcgttggttg
     1741 gaaaccccaa cgccggaaaa acctccattt tcaaccttgc gtcgggggca catgagcatg
     1801 tgggaaatta cagcggcgta acggtcgatt ccaaagaagg aacgctgagg cacaacggtt
     1861 ataagtttat attgaccgat cttccgggaa catattcatt gtctgcttat tctcccgagg
     1921 aactgtacgt aagaagttat atttttgaag aaaaacccga tgttattgtg aatgtggtgt
     1981 cggcatcggc cttggagaga aacctgtatc ttaccaccga gcttattgag ttggagaaac
     2041 caatggtagt agcactcaat atgtacgacg aacttgagaa gagcggccgc ttattcaagc
     2101 accagacgct ttctgaaatg ctgaatgttc ctattgttcc tacggtggga aagaaagggt
     2161 tcggtattcc cgaattgctc gaaaatgtga ttagcattta cgaatccggc aacaactctc
     2221 acaacgtaaa ggttccctac gggcgtgtat tggagaaatc catcggcttt atgtgtcgcg
     2281 atttattatc caacggcttt tcgacgttag gtatgccgaa gcgttatgtc ggtattaagt
     2341 tgctggaggg ggataaagag gtagaaaatg ctattcgtga gcacgataaa ggcaaggagc
     2401 ttctggctcg tagaaacaag gaacgggaat atatcgaaag attgttgaaa gaagacccag
     2461 agtcggcctt taccaatgcc cggtatggat ttattgcagg agcactcaaa gagacactga
     2521 gcgaaaaaac aaaattcgaa gataaaacta ctgtgctcga tgctgtcctg acgaataaat
     2581 atttggggct cccgcttttc tttgtctttc tgtggataat gtttgaagct acattccggc
     2641 ttggagctta tccgatggag tggattgagt ggattgtggc ccaggcaggg aatttgattc
     2701 gtgtaaatat gacggaagga ccactcaagg atttgctggt agacggcgtt atcggcggag
     2761 tggggggtgt tattgttttt ctgccgaaca ttgtgatatt gtacgcgttt atcgcgttta
     2821 tggaagattc gggatacatg gcccgggcag cgttcatcat ggataaactg atgcataaca
     2881 tcggccttca cggaaaatcg tttattccgc ttatcatggg gtttgggt
//
LOCUS       DAOSDR010000390         2925 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrimonas sp. isolate AMR_MDS_1826
            NODE_11966_length_2925_cov_3.853310, whole genome shotgun sequence.
ACCESSION   DAOSDR010000390 DAOSDR010000000
VERSION     DAOSDR010000390.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863355
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrimonas sp. (wastewater metagenome)
  ORGANISM  Petrimonas sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Dysgonomonadaceae; Petrimonas.
REFERENCE   1  (bases 1 to 2925)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2925)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:10:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,720
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 35
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2925
                     /organism="Petrimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11966_length_2925_cov_3.853310"
                     /isolate="AMR_MDS_1826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2023866"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..410
                     /locus_tag="PLI30_12350"
     CDS             <1..410
                     /locus_tag="PLI30_12350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI80380.1"
                     /translation="NSATGSSSVSSADLYDLYDLEFNGTEVVALYKDYTANRTATIEV
                     SAATALFTNPQDIVTAVTPHGLSGTSSATLVAGLAASNLFTKYNTMENTESGVTVKTG
                     TRVAAVNIGTDFFSVYTDNADSKIKILKIIIPL"
     gene            complement(474..1466)
                     /locus_tag="PLI30_12355"
     CDS             complement(474..1466)
                     /locus_tag="PLI30_12355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018696169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-1,6-bisphosphate aldolase"
                     /protein_id="HOI80381.1"
                     /translation="MVSYKELGLVNSKELFKKAIEGGYAIPAFNFNNMEQLQAIVSAC
                     VATKSPVILQVSSGARKYANQTLLRYMAQGAVQYAKELGYEIPIVLHLDHGDSFELCK
                     DCIESGFSSVMIDGSHLPYEENIALTKKVVDYAHQFDVTVEGELGVLAGIEDDVQAEH
                     HTYTEPDEVVDFVTRTGVDSLAISIGTSHGAFKFTPDQCTRDENGKLVPPMLRFDILK
                     EIEERIPGFPIVLHGSSSVPQEYVDIINQYGGKLKDSIGIPEEWLREAAKSAVCKINI
                     DSDGRLAMTAAVREVFANQPAEFDPRKYLGPARESLKKLYMHKTEKVLGSAGKA"
     gene            1737..2894
                     /locus_tag="PLI30_12360"
     CDS             1737..2894
                     /locus_tag="PLI30_12360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004585530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P) transhydrogenase subunit alpha"
                     /protein_id="HOI80382.1"
                     /translation="MIVGIPKEIMSGEARVAATPETVKKFIQDGMTVLVEAGAGVKSL
                     YYDEQYREAGAEMVGDAEHLFERADLILKVKEPLFNENVGKHEVEMMHPGQFLITFIH
                     PASPVNHNMVKQLAARGVTGLTLDSIPRISRAQNMDALTSMSTCAGYKGIIMAADDLS
                     VFMPQMFTAVGMLKPANVLVIGAGVAGLQALATAKRLGAVTYAMDIRPAASEQAKSVG
                     ARIIETGIPEELAVGKGGYAQKLPEEWLAKEREVLASVLKDMDIVFCSALIPGKVAPV
                     LITGEMVESMKNGAVIVDISIDQGGNCEVTPAGRKEVIHNVMVQGIKNIPGLIPTSST
                     WMFSQNVYNLVKYLSKDGEVTLDLNDEIVRSILVTHKGEIVHEGTREAMGL"
BASE COUNT          776 a          684 c          736 g          729 t
ORIGIN      
        1 ccaattcggc taccggaagc tcatccgtat catccgcaga tctttacgat ttgtacgacc
       61 tggagtttaa cggtactgag gttgtcgccc tttataagga ctacaccgcg aaccgcacgg
      121 ctactattga ggttagtgcc gcaaccgctt tgtttacaaa cccgcaagac attgtaactg
      181 cagttacccc tcacgggtta tcgggcacat cgtctgcaac gcttgttgcc ggcttggcgg
      241 cttcaaactt gttcactaag tacaacacga tggaaaatac cgaatcggga gtgacggtga
      301 agacaggtac ccgggttgcg gcggtgaata tcggcacgga tttcttttcg gtctataccg
      361 ataacgccga ctcaaagatc aaaatcctga aaatcataat acccctataa aagtaaaaat
      421 ccccggccag tatagccggg gattttagta taattcagaa accgtaaagt ggattacgct
      481 ttgcctgcgc tgccgagtac tttctcggtt ttgtgcatat agagtttctt caacgattcg
      541 cgagccgggc ccaggtattt gcgcgggtca aactcagcgg gctggttggc gaacacttca
      601 cgaacggcag ctgtcatagc caggcggccg tcggagtcga tgttgatttt acatacagcc
      661 gatttggcag cttcacgcag ccattcttcg ggaataccga tcgagtcttt cagcttgccg
      721 ccgtattggt tgatgatatc cacgtattcc tgtggaaccg aagaagaacc gtgcagaacg
      781 atggggaatc cgggtatacg ctcttcgatc tctttcaaaa tgtcgaaacg cagcataggg
      841 ggaaccaact tgccgttctc gtcgcgtgtg cactggtcgg gcgtaaattt aaacgctccg
      901 tgcgatgttc cgatggagat agccagagaa tcaactccgg tgcgggtgac aaaatcaacc
      961 acttcatccg gttcggtgta cgtgtggtgt tcggcctgaa catcgtcttc gataccggcc
     1021 aaaacgccca gttcaccttc aaccgtcacg tcgaattggt gcgcatagtc caccactttc
     1081 ttggtcaacg cgatgttttc ttcgtacgga aggtgcgaac catcaatcat aaccgaagag
     1141 aaaccgcttt cgatacaatc cttgcacaat tcaaagctgt cgccgtggtc caggtgaagg
     1201 acaatgggta tctcatatcc cagttccttt gcatactgaa cggcaccttg agccatataa
     1261 cgcagcaggg tctgattggc atacttacgc gcaccgctgg aaacctgcaa aattacgggc
     1321 gactttgttg ccacgcaagc agaaacaatt gcctgcaact gttccatgtt gttaaaattg
     1381 aaagcaggaa tagcatagcc tccctcaatc gcttttttaa acaattcttt tgagtttacc
     1441 aaacccaact ctttgtaact taccatattc ttgttattta aaaaattaat gaattacgtt
     1501 cccaaaacaa ggtacaaaaa taacatttat tctgcacaaa cggaaatttt ttctcctaag
     1561 agaatgtaaa agagttgagt ttattagaga tggagtctca tatttttgtt aaaactgtcg
     1621 ttatgtcatt tttttctcct atttttgtga ttaatttttg agtggccttt acaaagagaa
     1681 acatccaaaa agttagtttt ctaatattat tttgaacaat aaattacaaa tcaattatga
     1741 tcgtagggat tcccaaagaa attatgtcgg gagaagctcg tgtggctgct acccccgaaa
     1801 ccgtgaagaa atttattcag gacggcatga ccgtgcttgt ggaagccggt gccggagtaa
     1861 aatcgcttta ttacgacgaa cagtatagag aagccggagc cgaaatggta ggcgatgcgg
     1921 agcacctctt cgaaagagct gaccttatcc tgaaagtgaa agaaccgctt tttaacgaaa
     1981 atgtgggtaa gcacgaagtg gagatgatgc atccggggca gttcctgatt acgttcatcc
     2041 atcccgcctc acccgtaaac cacaacatgg taaagcaatt ggccgcccgg ggcgtgaccg
     2101 gcctcacact cgatagcatc ccccgcatct cgcgcgctca aaacatggac gccctcacct
     2161 cgatgagtac ttgtgcaggt tacaagggaa tcatcatggc ggccgacgac ctgtcggtat
     2221 tcatgccgca gatgttcacc gccgtaggaa tgcttaagcc tgccaacgtg ctggttatcg
     2281 gtgccggtgt tgccggattg caggcgctgg ccactgccaa gcggctgggg gcagtgactt
     2341 acgccatgga cattcgtcct gctgccagcg aacaggccaa aagcgtcgga gccaggatca
     2401 ttgaaaccgg cattccggaa gagttggcgg tcggcaaggg cggatatgcg caaaagctcc
     2461 ccgaggagtg gttggcgaaa gagcgtgaag tgctggcaag tgtgctgaaa gatatggaca
     2521 ttgtgttctg cagcgcgttg attccgggaa aggtggcgcc ggttcttatt accggggaaa
     2581 tggtggaatc gatgaaaaac ggagcggtga ttgtggatat ctccatcgat cagggcggta
     2641 actgcgaggt taccccggca ggaagaaaag aggtgatcca caacgtgatg gttcagggaa
     2701 tcaagaatat accgggatta attcccacca gctctacctg gatgttctct caaaacgttt
     2761 ataacctggt gaaatactta tcgaaagacg gagaggtaac gctggacctg aacgatgaaa
     2821 ttgtccgttc tatcctggta acccataagg gcgagatcgt gcacgaggga acgcgtgaag
     2881 cgatgggatt gtgaaaaata aattttaatt gctgaggttg acatg
//
LOCUS       DAOSDR010000391         2898 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrimonas sp. isolate AMR_MDS_1826
            NODE_12119_length_2898_cov_3.673936, whole genome shotgun sequence.
ACCESSION   DAOSDR010000391 DAOSDR010000000
VERSION     DAOSDR010000391.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863355
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrimonas sp. (wastewater metagenome)
  ORGANISM  Petrimonas sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Dysgonomonadaceae; Petrimonas.
REFERENCE   1  (bases 1 to 2898)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2898)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:10:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,720
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 35
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2898
                     /organism="Petrimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12119_length_2898_cov_3.673936"
                     /isolate="AMR_MDS_1826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2023866"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..519
                     /locus_tag="PLI30_12365"
     CDS             <1..519
                     /locus_tag="PLI30_12365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005675690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl phosphate synthase large subunit"
                     /protein_id="HOI80383.1"
                     /translation="ADPVLGVDMASTGEVGCLGTHFDDALLTAMLSVGYRIPGKNIVV
                     SSGSTKSKVALLDACRLLVDNGYHLFATGGTQKFFEENGVKSTCVAWPDEEGEPKVTD
                     MIAEKKVDLVINIPKNLTERELTNGYKIRRGAIDFNIPLITNARLASAFIKAFCFMKA
                     EDIEIKHWGEYK"
     gene            complement(542..2146)
                     /locus_tag="PLI30_12370"
     CDS             complement(542..2146)
                     /locus_tag="PLI30_12370"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005820521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HOI80384.1"
                     /translation="MEKTKYIFVTGGVVSSLGKGIIAASLGKLLQARGFRVTIQKLDP
                     YINIDPGTLNPYEHGECYVTVDGQETDLDLGHYERFLGVETHRENNITTGRIYQNVIN
                     KERKGDYLGKTVQIIPHITDEIKENIKSFSRKGLDFVITEIGGTVGDIESLPYIESVR
                     QLKWELGKNCLIVHLTYVPYIAAAGELKTKPTQHSVKMLQEQGVQPDILILRTEHPIS
                     KELRKKIALFCNVEPQAVMQSIDASTIYRVPLNMQQEKLDEVVLTKMDVDLEKTPRAN
                     MEEWSGFVERFENATEEVRIGLVGKYVQLPDAYMSIIESLKHASAFHNRRLDLKLILS
                     DDITPENVGEKLQSLDGIVVAPGFGQRGMEGKFTALEFARENNIPCLGICMGMQTMSI
                     EFARNVLGYADANSTEIDPGTSHNVVDIMEEQKHILNLGGSMRLGAYTCQLKKGSKVN
                     AIYNKTEISERHRHRYEFNNAYRDEFEADGMICSGQNDTLNLVEIIELIHHKWYIGVQ
                     FHPEYSSTVIHPHPLFVSFVGAAKGL"
     gene            complement(2197..>2898)
                     /locus_tag="PLI30_12375"
     CDS             complement(2197..>2898)
                     /locus_tag="PLI30_12375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019538863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase-related protein"
                     /protein_id="HOI80385.1"
                     /translation="TVRASTPMYLLARYIRSMGVKMVLSGEGADEVFGGYLYFHKAPD
                     AEEFHKETVRKLSKLHQYDCLRANKSLAAWGVEGRVPFLDKEFLDVAMRLNPKDKMAG
                     NGKIEKHILRKAFEDYLPPEVTWRQKEQFSDGVGYGWIDSLRTIANETVTDEQMTNVR
                     YRFPINPPLTKEEYVYRTLYSDLFPSDSAAQCVPSVPSVACSTPVALEWDEAFKKNAD
                     PSGRAVGSVHERGYK"
BASE COUNT          723 a          754 c          643 g          778 t
ORIGIN      
        1 gcagaccccg tactgggagt ggatatggca tccacaggcg aggtgggctg cctcggaacc
       61 cacttcgacg acgcactgct taccgccatg ctttcggtgg gataccggat acccgggaaa
      121 aacattgtcg tttcttcggg aagtaccaaa tcgaaagtag cgctactgga tgcctgtcgg
      181 cttctggtcg acaatggata ccacttgttt gcaaccggcg gtacgcaaaa atttttcgag
      241 gaaaacggtg tgaaaagtac ctgtgtagcc tggccggacg aagagggcga accgaaagtt
      301 acggacatga ttgccgaaaa gaaagtggac ctggtcatca atattcccaa aaacctgacc
      361 gaaagggaat taacaaacgg ctacaaaatc cgtcggggag ctatagattt caacatcccg
      421 ctcataacca acgcccggct ggcgagcgcc tttatcaaag ccttctgctt catgaaggcg
      481 gaggatattg agatcaagca ttggggagag tataagtaga aaaccgggta gctgatgctt
      541 cctacaggcc tttcgctgcc ccaacaaagc tcacaaacaa cggatgcgga tggataacag
      601 tgctgctata ttcgggatgg aactgtacac caatgtacca tttgtggtgg ataagctcaa
      661 tgatttccac caagttgagc gtatcgtttt gcccgctaca gatcattcca tcagcttcaa
      721 attcgtccct gtaggcgttg ttaaactcat aacggtggcg gtggcgttca ctgatttcgg
      781 ttttgttgta aatggcgttc actttgctcc cttttttaag ctggcaggta taagcgccca
      841 accgcatact gccacccagg tttaggatgt gtttttgctc ttccatgata tccaccacgt
      901 tgtgcgaagt gccggggtct atctcggtac tgttggcatc ggcatagccc aaaacattcc
      961 gggcaaattc gatggacatc gtctgcattc ccatgcaaat accgaggcag gggatattgt
     1021 tttcacgggc aaattcaagt gcggtaaact tgccttccat accccgctgg ccgaacccgg
     1081 gtgcgacaac tattccgtcg aggctttgca gtttttcacc gacattttcc ggcgtaatat
     1141 catcagacaa aatcagcttc aggtcgagcc ttctgttgtg gaaagcactg gcgtgtttca
     1201 acgactcaat gatagacata taagcatcgg gcaactgaac gtacttaccg accaggccga
     1261 tgcgtacctc ttcggtcgca ttttcaaacc tttccacaaa accggaccat tcctccatat
     1321 tggcccgggg agttttttcc aaatcaacat ccattttcgt aagtaccacc tcatccaact
     1381 tctcctgctg catattcagc ggaacacgat aaatggttga agcatcaatc gattgcatca
     1441 cggcttgagg ctccacgtta cagaataaag caatcttttt ccgcaactct tttgaaatag
     1501 ggtgctccgt gcgcaggatc aggatatcgg gctgtacacc ctgctcctga agcatcttca
     1561 cggaatgctg cgtaggcttg gtctttaatt cccccgccgc cgctatatac ggaacgtagg
     1621 taaggtgtac gatcaggcag tttttgccca actcccactt taactgacgc acactttcga
     1681 tgtagggcaa actctcgata tcccctactg tacctccaat ttctgtgatg acaaagtcaa
     1741 gccccttccg actgaacgat ttgatgtttt cctttatttc atcggtaata tgcggtataa
     1801 tctgtaccgt ttttccgaga taatctcctt ttcgttcttt gttgattacg ttctgataaa
     1861 ttcgtccggt ggtaatgtta ttttcccggt gtgtttccac tccgaggaaa cgttcgtaat
     1921 gccccaaatc caaatccgtt tcctgtccgt ctaccgtaac ataacattca ccatgttcat
     1981 acggattcag tgttcccggg tcaatattga tataaggatc cagtttctgg attgtcaccc
     2041 tgaaaccgcg tgcctgcagc aatttgccaa gcgatgcggc gataatccct ttgcccaaag
     2101 acgacaccac accgcctgtc acgaatatgt atttagtttt ttccattttt gcggtaaaga
     2161 tacacatttc gataaaaatt tatttcattc ctacatttat ttgtaccctc tttcgtgcac
     2221 tgaacccacc gcgcgccccg acgggtcggc atttttctta aacgcttcat cccattcaag
     2281 tgcgacaggt gtgctgcagg ctaccgaagg aacagacgga acgcactgcg cagccgaatc
     2341 agaagggaac aggtctgaat acagggttcg gtaaacatat tcttcttttg tcagtggcgg
     2401 atttatagga aaacggtatc tcacgttggt catttgttca tcagtaactg tctcatttgc
     2461 aatggttctc aaggaatcaa tccagccgta acccacaccg tcggagaact gctccttttg
     2521 ccgccaagtc acttctggag gcagatagtc ttcaaaagcc ttacgcaaaa tatgtttttc
     2581 aatttttccg ttccctgcca ttttatcttt cgggttaagc cgcatggcta catccagaaa
     2641 ctctttgtct aagaaaggca cacgcccttc cacaccccat gcagccagcg atttgttggc
     2701 tcgcaaacag tcgtactggt gcaatttact taatttccgc acggtctcct tatgaaactc
     2761 ttccgcatcg ggagccttgt gaaaatagag ataaccaccg aaaacctcat cggcaccttc
     2821 gcccgaaagc accatcttca cccccattga acggatatag cgggccaaca ggtacatcgg
     2881 agtacttgcc cggacagt
//
LOCUS       DAOSDR010000392         2881 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrimonas sp. isolate AMR_MDS_1826
            NODE_12208_length_2881_cov_3.334041, whole genome shotgun sequence.
ACCESSION   DAOSDR010000392 DAOSDR010000000
VERSION     DAOSDR010000392.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863355
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrimonas sp. (wastewater metagenome)
  ORGANISM  Petrimonas sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Dysgonomonadaceae; Petrimonas.
REFERENCE   1  (bases 1 to 2881)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2881)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:10:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,720
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 35
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2881
                     /organism="Petrimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12208_length_2881_cov_3.334041"
                     /isolate="AMR_MDS_1826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2023866"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1388
                     /gene="fucI"
                     /locus_tag="PLI30_12380"
     CDS             <1..1388
                     /gene="fucI"
                     /locus_tag="PLI30_12380"
                     /EC_number="5.3.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010991938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008736 - L-fucose isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006005 - L-fucose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="L-fucose isomerase"
                     /protein_id="HOI80386.1"
                     /translation="KGLPAFGIYGRDVQDLDDNSIPDDVAEKLLRFARGAQAVATMRG
                     KSYLSIGNVSMGIAGSIVNPDFFQEYLGMRNEQVESIEILRRMEEGIYDREEYAKAMA
                     WTRKYCVPNEAEDLNRAEKVKNRAEKDADWEFVVKMTLIIRDLMTGNPSLKEMGFKEE
                     AIGHNAIAAGFQGQRQWTDYKPNGDFSEALLCSSFDWNGIREAYVVATENDACNGVAM
                     LFGHLLTNTSQIFSDVRTYWSPAAVKRVTGKELTGKAKDGIIHLINSGATTLDATGKQ
                     RKEGKPAMKPFWEINEKEVEDCLAATRWCPANRDYFRGGGYSSNFLSEGGMPVTMSRL
                     NLVKGVGPVLQIAEGWTVEIDPEIHKTLNERTDQTWPTTWFVPRLTSKPAFKDVYSVM
                     NNWGANHGAISYGHIGQDLITLASILRIPVCMHNVEEDEIFRPAAWNALGMDKEGADY
                     RACATFGPIYK"
     gene            1403..1828
                     /gene="fucU"
                     /locus_tag="PLI30_12385"
     CDS             1403..1828
                     /gene="fucU"
                     /locus_tag="PLI30_12385"
                     /EC_number="5.1.3.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004114069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016854 - racemase and epimerase activity
                     [Evidence IEA];
                     GO_function: GO:0042806 - fucose binding [Evidence IEA];
                     GO_process: GO:0042354 - L-fucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose mutarotase"
                     /protein_id="HOI80387.1"
                     /translation="MLKKIPKVLSPQLVKALMEMGHGDEIVLGDANFPGCSLSTNVIR
                     ADGLSGAVLLKAILELFPLDPYSEHSVFLMEVTPGDDYKPDIWEEYKEILSASGEPFS
                     IGHLERFAFYNRAKTSFAVIASGEEALYANIILKKGVVR"
     gene            1825..>2881
                     /gene="fucP"
                     /locus_tag="PLI30_12390"
     CDS             1825..>2881
                     /gene="fucP"
                     /locus_tag="PLI30_12390"
                     /inference="COORDINATES: protein motif:HMM:TIGR00885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015535 - fucose:proton symporter activity
                     [Evidence IEA];
                     GO_process: GO:0015756 - fucose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose:H+ symporter permease"
                     /protein_id="HOI80388.1"
                     /translation="MKHSTSPSKPAVIEKKYLVPFILITSLFALWGFANDITNPMVAA
                     FQTIMEIPVSEAALVQFAFYGGYGTMAIPAALFVRRFSYKKGILLGLALYAIGAFLFI
                     PAAQFEQFTFFLLSLYILTFGLAFLETTANPYIISMGDEATSTRRLNFAQSFNPMGSL
                     LGMTVASNYVLTSLDSEKRDAAGNLIFHSLGEAEKAVIRTHDLEIIRNPYVIIGGVVL
                     LVFVIIALTKMPRTQSAEAKNIRAIDSARRLLRNHNYREGVLTQFFYVAAQIMCWTFI
                     IQYAENIGIPKAVGQRWNILAMSLFLTSRFISTYLMKYLRPSLMLTLFAAGAMATTLG
                     VIFIGGMTGLYCLVATSV"
BASE COUNT          733 a          747 c          720 g          681 t
ORIGIN      
        1 aaaaagggtt gcctgccttt ggtatttacg ggcgcgacgt gcaagacctg gacgacaatt
       61 ccattcccga tgacgtggct gaaaagctgc tgcgctttgc ccgcggagcg caggctgtcg
      121 ccaccatgcg cggaaaatcc tacctatcga taggaaatgt atctatgggt attgccggtt
      181 ccattgtcaa tcccgatttc ttccaggaat acctggggat gagaaacgaa caagtggaat
      241 ccatcgaaat tcttcgtcgc atggaagaag gaatttacga ccgggaagag tatgccaaag
      301 caatggcatg gacaagaaaa tattgtgtcc cgaacgaagc cgaagacctc aaccgggctg
      361 aaaaagtaaa gaaccgtgcc gaaaaagatg ctgattggga atttgtggta aagatgaccc
      421 ttatcatccg cgacttgatg accggaaacc ccagtctcaa ggagatggga ttcaaagaag
      481 aggccatcgg acacaacgct attgccgccg gttttcaagg acaacgccag tggaccgact
      541 ataaaccgaa cggcgacttc tccgaagccc tgctctgttc atcttttgac tggaacggaa
      601 ttcgtgaagc ctatgtggta gctaccgaaa acgatgcctg taacggcgtg gccatgttgt
      661 ttggacatct tctgaccaac acttcccaga tcttctccga tgtacgcacg tactggagtc
      721 ctgcggcagt gaaacgggtt acgggaaaag agcttaccgg aaaagccaaa gacggaatca
      781 ttcacctcat caactccggc gccaccacgc tcgacgcgac cggaaaacaa cggaaagagg
      841 gcaaaccggc catgaaaccg ttctgggaaa tcaacgaaaa agaggtagag gactgtctgg
      901 cggccacgcg ctggtgtcca gcaaaccgcg attatttccg cggcggaggc tactcttcca
      961 atttcctctc ggaaggcggg atgcccgtta ccatgtcacg cttaaacctg gtaaaaggtg
     1021 tcggtccggt gttgcaaata gccgaaggat ggaccgtgga gatcgatccg gagattcata
     1081 aaacgctcaa cgaacgcacc gaccagacct ggcccaccac ctggttcgtc cccaggctga
     1141 ccagcaaacc ggccttcaag gatgtctact cggtaatgaa caactgggga gcaaaccacg
     1201 gggccatcag ctacggacac atcggacagg acctgatcac gctggcctcc attcttcgca
     1261 ttccggtgtg tatgcacaac gtggaagaag acgagatatt ccgccccgca gcatggaatg
     1321 cattgggaat ggataaggaa ggtgccgact accgcgcctg cgccaccttt ggccccattt
     1381 acaaataaga tttgcaataa ctatgctcaa aaaaataccc aaagtgctat cgcctcagct
     1441 ggtaaaagcc ctcatggaga tggggcatgg cgatgagatc gttcttggtg acgccaattt
     1501 tccgggctgc agcctttcaa ccaacgtcat cagggctgac gggctctccg gtgctgtctt
     1561 actgaaagcc attcttgagc ttttcccatt agacccttat tccgaacatt cggtttttct
     1621 gatggaagta actcccggcg acgattataa accggatata tgggaagaat acaaagagat
     1681 tttgagtgct tcgggtgaac ctttctctat cggccacctg gaacggtttg ctttttacaa
     1741 cagggcaaaa acatcttttg ccgtaatcgc ttccggcgaa gaggcgcttt atgccaatat
     1801 catcttaaaa aaaggagtgg tcagatgaaa cacagtacgt caccttccaa accggcggtt
     1861 attgagaaaa aatacctcgt cccctttata cttatcacca gtttatttgc cctgtggggg
     1921 tttgccaacg acatcaccaa tcccatggtg gctgcttttc agaccattat ggagattccg
     1981 gtttcggaag cggcattggt gcaatttgcg ttttacggcg gctacggcac catggctatc
     2041 cctgcagcgt tattcgtcag gcggttcagt tacaagaaag gcatacttct gggattggcc
     2101 ctgtatgcca ttggggcatt tttatttatc cccgcggccc agttcgaaca attcacgttt
     2161 ttcctgctgt cgctctatat cctgactttc ggactggctt ttttggaaac aaccgccaat
     2221 ccgtacatca tttcgatggg tgatgaagct acttctacac gcaggctgaa ctttgcccaa
     2281 tcgtttaacc cgatgggctc attgttaggc atgactgtag catcgaatta cgtgcttacg
     2341 tcccttgact cggagaaacg cgatgcggca ggcaacctta tcttccattc attaggtgaa
     2401 gctgaaaagg ctgttatccg gacacacgac ctggaaatca tccgaaaccc gtatgtgatc
     2461 atcggcgggg tcgttttgct ggtcttcgtc attatcgctt tgactaaaat gcccaggacc
     2521 caatcggcag aggcgaaaaa tatccgtgcc atagattcag ccaggagatt actccggaac
     2581 cacaattacc gcgagggggt tctcacacaa ttcttctatg tagcggcaca aatcatgtgc
     2641 tggacattta ttattcaata cgccgagaac atcggtatac cgaaagccgt aggccagcgc
     2701 tggaacatcc tcgccatgtc cctgttcctg acgagccgct ttatcagcac ttacctgatg
     2761 aagtacctca ggccatcact tatgctgacc ctgtttgcag cgggggccat ggctaccacc
     2821 ctgggggtta tcttcatcgg cgggatgacc ggtttgtatt gcctggtagc cacctccgtt
     2881 t
//
LOCUS       DAOSDR010000393         2881 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrimonas sp. isolate AMR_MDS_1826
            NODE_12209_length_2881_cov_2.670559, whole genome shotgun sequence.
ACCESSION   DAOSDR010000393 DAOSDR010000000
VERSION     DAOSDR010000393.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863355
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrimonas sp. (wastewater metagenome)
  ORGANISM  Petrimonas sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Dysgonomonadaceae; Petrimonas.
REFERENCE   1  (bases 1 to 2881)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2881)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:10:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,720
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 35
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2881
                     /organism="Petrimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12209_length_2881_cov_2.670559"
                     /isolate="AMR_MDS_1826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2023866"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..213
                     /locus_tag="PLI30_12395"
     CDS             <1..213
                     /locus_tag="PLI30_12395"
                     /inference="COORDINATES: protein motif:HMM:NF012422.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HOI80389.1"
                     /translation="KEEKISAPSLTKREREVLKLVAEGKTNREIGAELFIAEITVGKN
                     ITSIYQKLGVTGRPSAVKKAIELNIL"
     gene            268..555
                     /locus_tag="PLI30_12400"
     CDS             268..555
                     /locus_tag="PLI30_12400"
                     /inference="COORDINATES: protein motif:HMM:NF021873.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2442 domain-containing protein"
                     /protein_id="HOI80390.1"
                     /translation="MIISFQGLLFLLQMGGVDMPKIMRVEPKDDHTLLIELNNKHMIV
                     YDMKPRLMTVRFCGLADLDRFKAVRVEDENTLVWDSLCQMSIDEILNMLER"
     gene            527..>2881
                     /locus_tag="PLI30_12405"
     CDS             527..>2881
                     /locus_tag="PLI30_12405"
                     /inference="COORDINATES: protein motif:HMM:NF019217.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GLUG motif-containing protein"
                     /protein_id="HOI80391.1"
                     /translation="MRYLTCLKDDSIWRAECKLIELLQFILGKEVNDLKNKDNNIQLP
                     VKTIAITVMLLMAAFLLIPGTVLAEGGFAGGSGTVEEPWLVETPGQLATVAEEVYRDG
                     HFKLIDDIDLSDYLSDGGGGWNEGAGWKPIGTYDDPFTGSFDGDGHTISGLFIYRGDT
                     EGVGLFGYTDGATIKDVKLDNADITGDAYVGCLVGDKENSTISGCSAENVKVTGFNSV
                     GGLVGYNTSGNITNSFATAEVSGVFAVGGLVGNNFGGSIESSYATGTVSDIDSDDPED
                     SGHFGGLAGRNRANIANSYATCVVNGTDCVGGLVGENTEEGVIANSYATGPVTGNANV
                     GGLVGRNSLDDDSFDNPLIIYCYAAGSVTGNANVGGLVGGNFDGVIENSFYDRDTSGR
                     SDEDKGERKSTAEMNTIATFFYAGWDFIGEMENGDEDIWGIDESADATDNDGYPFLVW
                     QDYDPIIWPSVSTIGAENITDTTADGKGEIIIIGYPNATQHGVVWSVEENPTLEDNKT
                     EEGVVLGDEPFTSEITGLEQDTTYYVRAYATNTLGTVYGVEISFTTEESDFAGGDGSS
                     DNPYQITAAEHLANVSEYMDSYFVLVDNIDLSAYGDGYDGGAGWEPIGTQVNPFTGSF
                     DGNGHTITGLYINRPDTYYVGIFGCTGADAEISNLRLLNVDVSGYGGVGALVGENHGT
                     ITGSCAAGSVEGSDYVGGLVGRNGEVATCSIIDCYSAVNVVGAESVGGLAGGNEGTIT
                     GSYSTGDVVGEEYIGGLTGGSGTIDYRCAISDSYATGNVTGIRYV"
BASE COUNT          775 a          542 c          821 g          743 t
ORIGIN      
        1 aaagaagaaa aaatatccgc tccttccctg acgaagcggg aaagggaagt gctgaagctt
       61 gttgcggagg ggaaaaccaa cagggaaatc ggggctgaac tttttattgc cgagataacc
      121 gtcggtaaaa atattacatc catttatcaa aagcttggtg tcacaggcag gccttctgcc
      181 gttaagaaag caatcgagtt gaatattttg taagtttttc tcaaaaagta taggatccta
      241 taatatgaag attttttaaa ccctagtatg attatatcat tccagggttt attatttttg
      301 ctgcagatgg gaggagtcga tatgcccaag ataatgcggg ttgaacctaa ggatgaccac
      361 acactgctta ttgagctgaa taacaaacat atgattgtct acgacatgaa gcccaggctg
      421 atgacggtac gtttctgcgg acttgccgat cttgaccgct tcaaggctgt cagggtagag
      481 gatgaaaata cccttgtctg ggacagcctg tgtcaaatga gcatcgatga gatacttaac
      541 atgcttgaaa gatgacagta tatggcgagc tgaatgcaaa ttaatcgaat tactgcaatt
      601 tattttagga aaggaagtga atgacttgaa aaacaaggat aataatatcc aattgccggt
      661 taagacaata gctattacag tgatgctcct gatggcagct ttcctgctga ttcccggtac
      721 ggttttggcg gaaggcgggt ttgcaggggg atcgggtacg gtggaagaac catggcttgt
      781 tgaaactccc gggcagctgg caacagttgc ggaagaggtt tatcgcgacg gtcattttaa
      841 attgatagac gatattgatc tgagcgatta tctatctgat ggaggtggcg gctggaatga
      901 aggtgcaggc tggaagccca ttgggacgta tgatgatcct ttcacaggca gctttgacgg
      961 tgacgggcat accataagcg gcctctttat ttatagaggt gatacggagg gtgtcggcct
     1021 gttcggttat acggatggtg caactattaa ggatgtgaaa ttggataatg ccgatattac
     1081 cggtgatgcc tacgttggct gtctggtggg ggataaagaa aacagtacga tcagcggttg
     1141 ttccgcagaa aatgtgaaag taaccggctt caattcagtc ggcggacttg tgggctataa
     1201 cacatcgggg aatattacca actcattcgc cacggctgaa gttagtgggg ttttcgcggt
     1261 tggcgggtta gttgggaaca actttggcgg ttcaatcgaa agttcctatg ccacaggcac
     1321 tgtgtcggat attgattccg atgatcccga agattcggga cacttcggtg gcttggcagg
     1381 acgtaacaga gctaatatcg caaactctta cgccacctgc gttgtaaacg gcactgattg
     1441 tgtcggcgga ttggtggggg aaaatacaga ggagggagtt atcgcaaact cttacgccac
     1501 gggtcccgtg acaggcaatg ctaatgtcgg cggattggtg gggcgcaact ctcttgatga
     1561 tgattctttc gataacccac ttattatata ctgctatgct gcaggttccg tgacaggcaa
     1621 tgctaatgtc ggcggactgg tgggagggaa tttcgatggc gttattgaaa actcctttta
     1681 cgacagagat acttccggcc gatcggatga agacaaaggt gagcgtaaaa gtacggctga
     1741 aatgaatact attgcaacat ttttttatgc cggttgggat tttataggtg aaatggagaa
     1801 cggagatgaa gatatttggg gtattgacga atctgcagat gccactgaca atgacggata
     1861 tcccttcctg gtttggcagg actacgatcc tattatatgg ccttccgtat ctaccatagg
     1921 agcggagaat attacagata caactgctga tggtaagggg gagattatta ttataggcta
     1981 tcccaatgct acacagcatg gggttgtctg gagcgttgaa gagaatccca cactggaaga
     2041 taataaaaca gaagaaggag tagttttggg agacgaacca tttacctcgg aaattaccgg
     2101 gttggagcag gatactactt attatgtaag agcatacgct acaaatacct taggaacagt
     2161 ttacggagta gaaatcagtt ttacaacaga ggagtcagat tttgccggcg gggatggatc
     2221 atctgataat ccctaccaaa taacggctgc ggagcatttg gccaatgtat cagagtacat
     2281 ggacagttat tttgtcctgg tggataatat tgacctttcc gcttatggag acggatatga
     2341 tggcggcgca ggctgggagc ccattgggac gcaagtaaat ccctttacag gcagttttga
     2401 cggcaacgga cacaccatca ccggactcta catcaaccgt cctgatacat attacgtggg
     2461 gatattcggc tgtacgggag cggatgctga gattagtaac cttaggctgc tgaacgtgga
     2521 tgtgtccggc tatgggggtg tcggcgctct ggtgggagaa aatcatggca ctataaccgg
     2581 ttcctgcgct gcgggcagcg tggagggcag tgattatgtc ggaggcttgg ttggaaggaa
     2641 tggggaggtt gctacctgca gcattattga ctgttattcc gccgttaatg tggttggtgc
     2701 agagagtgtc ggcggcctgg ccggaggaaa cgaaggcacc ataaccggtt cctattccac
     2761 gggggatgtt gtgggtgagg aatatatagg cggactgaca ggaggcagcg ggacaataga
     2821 ttacaggtgt gccattagcg actcatacgc cacgggtaat gtcaccggta tcagatatgt
     2881 c
//
LOCUS       DAOSDR010000394         2861 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrimonas sp. isolate AMR_MDS_1826
            NODE_12311_length_2861_cov_3.297933, whole genome shotgun sequence.
ACCESSION   DAOSDR010000394 DAOSDR010000000
VERSION     DAOSDR010000394.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863355
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrimonas sp. (wastewater metagenome)
  ORGANISM  Petrimonas sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Dysgonomonadaceae; Petrimonas.
REFERENCE   1  (bases 1 to 2861)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2861)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:10:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,720
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 35
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2861
                     /organism="Petrimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12311_length_2861_cov_3.297933"
                     /isolate="AMR_MDS_1826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2023866"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..964)
                     /locus_tag="PLI30_12410"
     CDS             complement(<1..964)
                     /locus_tag="PLI30_12410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HOI80392.1"
                     /translation="MNLFKKTGIIIALTIVVFMGVNAQSVSGDWKGTLSVQGMNLDLI
                     FHLAEENGSYSGTLDVPVQGATGIPVDKVETDGKTIKLGVSAAQIIYNGALQGDSIVG
                     NYEQAGMSLPLTLKRFESKLPGITSLVSSEEELQALKLSDKGDYKYRVEDYFAKPKAS
                     SFQLSPNGKYLSYMEKDGLKNHVYIKEIATGKVTRAIEEKEEPIKGYGWVNDSRLIFV
                     MDKGGNENYHIYAANTDGSNLMDLTPFEGVRAMMLNELKDQKDYIIISMNKDNKQVFE
                     PYKLNVNTGALEKLFENNDPANPIQGYDFDKDGTLRGYTKMVNGV"
     gene            complement(1029..2204)
                     /locus_tag="PLI30_12415"
     CDS             complement(1029..2204)
                     /locus_tag="PLI30_12415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005787341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HOI80393.1"
                     /translation="MKAFIFIILYIAPFVALAQDRYAAVLQRIETNNTTLAALREQTE
                     AQKIENRTGIYISNPEVEFNYLWGNPAAIGRRTDLAVTQSFDFPTAYGHRRNIGDLQS
                     RNADLAYKAERTRLLLQAKQTCIDLVHYNALAKEYGIRLENAQRIADTYRIRLDKGDA
                     DILEYNKAKLNLTAIQTERAKVEAEQVALLAELKRMNGGKEIFFTQDEFTPPVLPADF
                     EEWYSNAEAKNPVLQYVKGEVNIGKEQVKLSRALSLPKFTTGYMSETVVGEQFQGITL
                     GVSIPLWENKNRQRHAQARVKASETALEDTRLQFYTRLQGLYHKASILQQNALELRRS
                     ITENRNDALLKKALDSGEISLLDYLMEIGYYYDAVIQALVVERDYEQALAELSAVEL"
     gene            complement(2201..>2861)
                     /locus_tag="PLI30_12420"
     CDS             complement(2201..>2861)
                     /locus_tag="PLI30_12420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018337521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HOI80394.1"
                     /translation="ENVQRKIVVSANTSGRDLKSVVDDIRQNIEENVVLPEGYRIEYG
                     GQFETAANATRTLLIASLLAVCVIFLLLYGEFKDLTLSAIVLLNLPLALIGGVLAVFF
                     TSGVISIPSIIGFITLFGIATRNGILLISRYQHMQQDGEPLHQRVLHGSTDRLNPILM
                     TALTAALALIPLVFQGGKPGNEIQSPMAVVVLGGLLTSTILNIFIVPIVYETIEKKRK
                     K"
BASE COUNT          706 a          732 c          613 g          810 t
ORIGIN      
        1 caacaccgtt caccattttg gtgtagccgc gcaaagtgcc gtctttgtca aaatcatatc
       61 cctgaatggg gttggccgga tcgttattct caaaaagctt ctccagagct ccggtgttta
      121 cattcaattt atagggttca aaaacctgtt tgttgtcttt gttcatcgag atgatgatgt
      181 aatctttctg gtctttcaat tcattcaaca tcatcgctct taccccttca aagggagtca
      241 ggtccatcaa gttgctgccg tcggtatttg ccgcataaat atggtagttc tcgttgccgc
      301 ccttatccat gacaaatatc aaacggctgt cgtttaccca cccgtaaccc ttaataggct
      361 cctccttctc ttcaatggcc cgggtgacct tgccggtggc gatctctttt atatacacgt
      421 gatttttcaa cccatccttc tccatatacg acaaatattt gccgttcggc gaaagctgaa
      481 aagaagaggc tttcggcttg gcgaaataat cctccaccct gtatttgtaa tctcccttgt
      541 cggatagctt caaggcttgc agttcctctt cggacgaaac caaggacgta attccaggta
      601 gcttgctctc aaatcttttt aaggtcagcg gcaacgacat tccggcttgt tcgtaatttc
      661 caacgatgct gtcgccttgt agtgctccgt tatagatgat ttgcgccgcc gatactccca
      721 gcttaattgt ttttccgtca gtttccacct tgtccaccgg aattcccgtt gcgccctgca
      781 cgggcacatc cagcgtgccg gaataacttc cgttttcttc ggccagatga aaaataagat
      841 cgaggttcat tccttgaacc gataaggttc ccttccagtc tcctgaaaca ctttgtgcat
      901 ttacacccat aaaaacaaca attgttaagg caatgatgat gcccgttttt ttaaatagat
      961 tcatgatact tggtttttac tataaaacaa tcgatcaaat aaattgtttt ggacacagga
     1021 accacgtatt atagttccac tgcggataat tccgccaaag cctgctcgta atcacgctca
     1081 actaccaacg cttgtataac ggcatcgtaa taatacccga tctccatcag gtaatccagc
     1141 aaggaaattt ctccgctgtc cagcgctttt ttcaggagcg cgtcgttcct gttttccgtt
     1201 atcgaccggc gaagttccaa tgcgttttgc tgcaatatcg atgctttatg gtaaaggccc
     1261 tgcagccggg tatagaactg tagccgggta tcctccagtg ccgtttccga agcctttacc
     1321 cgggcctgtg catgccgttg cctgttcttg ttctcccaaa gcgggatgga aactcccagc
     1381 gtaataccct ggaattgctc tccaaccact gtttcactca tatacccggt tgtgaactta
     1441 ggcaacgaca aggcacggct cagctttacc tgttcctttc ctatattgac ttctcccttt
     1501 acgtattgca atacgggatt cttcgcttcc gcgttcgaat accattcttc gaaatctgca
     1561 ggaagtaccg gaggagtgaa ctcatcctgt gtaaaaaaga tttcctttcc gccgttcatt
     1621 cttttcagtt cagccagcaa agctacctgt tcagcctcta cctttgcccg ttcggtttgt
     1681 atggcggtca ggttcagctt ggccttattg tactcgagaa tatccgcatc acccttgtcc
     1741 aacctgatgc ggtaagtgtc cgcaatcctt tgtgcattct ccaggcgtat cccatattct
     1801 tttgccaggg cattgtaatg caccagatca atacaggttt gcttggcttg aagcaacagg
     1861 cgggttcgct ctgccttgta agcgagatcg gcattcctgc tttgcaggtc gccaatgttt
     1921 ctgcgatggc cgtaagccgt tggaaaatca aaggactgag taaccgcaag atcggttcga
     1981 cggccgatag ccgcgggatt tccccaaagg taattgaatt caacttccgg attagagata
     2041 tagattccgg tacggttttc gattttctgc gcttccgttt gttcccgtaa tgcagccaag
     2101 gtggtattat ttgtttcaat acgctgcaaa acagcggcat atctgtcttg ggcaagagcc
     2161 acaaaaggag caatataaag gatgataaaa ataaaagctt tcattttttt ctttttttct
     2221 ctattgtttc atacacgatg ggaacaataa aaatattcag gatagtggag gtaagcaaac
     2281 cgcccagcac aactacagcc atggggcttt ggatttcatt tccgggtttc ccgccctgaa
     2341 aaacaagggg tataagagcc agagcagccg tgagcgcagt catcagaatg gggttaagac
     2401 gatcggtgga accgtgcaac accctttggt gaagaggctc cccatcctgc tgcatgtgct
     2461 gatacctcga tatcagcaag attccatttc gcgtggcaat accaaaaagg gtaataaaac
     2521 cgatgatgga cggtatgctg ataacaccgg aagtaaaaaa cacagccagg accccaccaa
     2581 tcaatgccag cggaaggttg agcaaaacga tggccgagag agtcaggtcc ttgaattctc
     2641 cgtacagcag caagaagatg acacaaactg ccagaagcga tgcaatcaat aacgtgcgtg
     2701 tagcgtttgc cgctgtctca aactgcccac cgtactcaat cctatagcct tcgggcaaga
     2761 cgacattctc ctcaatgttt tgccggatat catccaccac acttttcaag tcgcgtcccg
     2821 acgtatttgc ggaaacgaca atcttgcgct gcacattttc a
//
LOCUS       DAOSDR010000395         2858 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrimonas sp. isolate AMR_MDS_1826
            NODE_12326_length_2858_cov_4.768462, whole genome shotgun sequence.
ACCESSION   DAOSDR010000395 DAOSDR010000000
VERSION     DAOSDR010000395.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863355
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrimonas sp. (wastewater metagenome)
  ORGANISM  Petrimonas sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Dysgonomonadaceae; Petrimonas.
REFERENCE   1  (bases 1 to 2858)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2858)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:10:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,720
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 35
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2858
                     /organism="Petrimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12326_length_2858_cov_4.768462"
                     /isolate="AMR_MDS_1826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2023866"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..857
                     /gene="gcvT"
                     /locus_tag="PLI30_12425"
     CDS             <1..857
                     /gene="gcvT"
                     /locus_tag="PLI30_12425"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007652881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HOI80395.1"
                     /translation="VGKIQYTCFPNEQGGIKDDFLLYHFEPEKYMMVVNAANIEKDWE
                     WCQKQNTMGAVIEDASAHTALLAVQGPKAKDTLQKITDTDLSSIPYYTFANGKVGGVD
                     RVIISNTGYTGAGGFELYFYPEDGLKIWEALFEAGAEFGIKPVGLGARDTLRLEMGFC
                     LYGNDLDETTTPLEAGLGWITKFTEGNSFIARELLEKQKAEGVTRKLCGFTLSDKGIP
                     RHGYEIVNAGDEPIGTVTSGTISPVLKAGIGLGYVKPEYAKAGTTIYIKVRNKNLKAE
                     VTQLPFRK"
     gene            867..1859
                     /locus_tag="PLI30_12430"
     CDS             867..1859
                     /locus_tag="PLI30_12430"
                     /inference="COORDINATES: protein motif:HMM:NF015651.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HOI80396.1"
                     /translation="MSKTKIATNLFFLLGLIILGFMLYKTGIGVIWNNIKLTNWWFAA
                     IIGIWAVVYFLNAIAFHTIIRDGSPEARSVGFLRTLKLTISGYAINLITPFGLMGGEP
                     YKIIELQPALGIQKATSTVLLYMMMHFVSHFIFWMVSIPLLFIIVPNVAVSVRIILAI
                     AGIASLLLLWWSFTVYSKGFVSKALSIAGKLPFAGKRVKVYKEKHLEKIEQMDFLIAN
                     LYKNRKKDFVTSLSLELLSRFVLCTEIIFMMHAIRFPVTFTQSVIVESIQSMVGNLFF
                     FMPMQLGAREGGFILVFGILSLPAAHAVYVSLCIRIRELFWTLFGLGLIQVRRR"
     gene            complement(1836..2597)
                     /locus_tag="PLI30_12435"
     CDS             complement(1836..2597)
                     /locus_tag="PLI30_12435"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOI80397.1"
                     /translation="MKHVIVTGGAQGIGRIIAQSLLKVGYAVSIFDMDNEAMEEIKPQ
                     FDKKRSAFFITDVSSEVSVRSSVRASLKKFDGIYGLVNNAVYEVFKPMSELTLEEWNR
                     AIGTNLTGTFLCAKFSSPHLIESRGSMVNMCSTRAFQSEPDTESYSASKGGIYSLTHA
                     LAMSLAPNVRVNSISPGWIDVSAVKKKAKARQVELSESDHAQHPAGRVGNAFDIAQMV
                     MFLLDSANGFITGQNFVIDGGMTKKMIYLPSPHLD"
BASE COUNT          728 a          720 c          702 g          708 t
ORIGIN      
        1 aggtgggaaa gatacaatac acttgttttc cgaacgagca gggtggcata aaggatgact
       61 tcctgcttta ccacttcgaa cccgaaaaat acatgatggt ggtaaacgct gccaacatcg
      121 aaaaggactg ggagtggtgt caaaagcaaa acacgatggg tgcagtgatc gaagatgcat
      181 ctgcccacac cgctttgctg gccgtgcaag gtccgaaagc caaagatacg ctgcaaaaaa
      241 ttaccgatac ggacttgtca tccatcccct actacacgtt tgccaacgga aaagtcggcg
      301 gagtagaccg ggtgattatc tccaacaccg gctacaccgg tgcgggcgga ttcgaactct
      361 atttttatcc ggaggacgga ttaaagatct gggaagcact gtttgaagcc ggagcggaat
      421 tcggcatcaa acccgtcgga ctgggtgcac gcgacaccct gcgccttgag atgggcttct
      481 gcctttacgg gaatgacctc gacgaaacga ctacccccct ggaagcgggg ctgggctgga
      541 tcaccaagtt tacagaaggg aactccttta ttgcaagaga gctgctcgaa aagcaaaaag
      601 cggaaggcgt tacgcgcaaa ttgtgtggat ttacgttgtc ggacaaggga ataccgcgcc
      661 acggttacga aattgtgaac gccggggatg aacccatcgg aaccgtaaca tcgggaacca
      721 tatcccccgt attgaaagcg ggcatcgggc tgggttacgt gaaacccgaa tatgcaaaag
      781 ccggaactac aatttacatc aaggtacgca acaagaacct gaaagccgaa gtcactcaac
      841 tacctttcag gaaatagcac actctcatga gtaaaacgaa gatcgccacc aacctgtttt
      901 tcctgctggg acttattatc ctgggtttca tgctatataa aaccgggata ggggttatct
      961 ggaacaacat aaaactgacc aattggtggt ttgcggcaat tatcggtatc tgggcggtgg
     1021 tctatttttt aaatgccatc gcctttcaca ccattattcg cgacggaagt ccggaagccc
     1081 ggtccgtcgg tttcctccgc acactgaagc tgaccatcag cgggtacgcc attaacctca
     1141 tcacgccttt cggactgatg ggcggggagc cttataaaat catcgaatta cagccggccc
     1201 tgggcattca gaaagccaca tccacggtac tcctttacat gatgatgcat tttgtatcgc
     1261 actttatctt ctggatggtc tccataccct tgctgtttat catcgtgccc aatgtagctg
     1321 tttccgtcag aataatcctt gccatagccg gcatcgcatc actgctgctg ctctggtggt
     1381 cgttcacggt ctactccaaa ggatttgtga gtaaggccct gtccattgcc gggaaactgc
     1441 cgttcgccgg aaaaagagta aaggtctaca aagaaaaaca cctggagaag atcgaacaga
     1501 tggatttcct gattgccaac ctgtacaaaa accggaaaaa ggattttgtt acgtcgctct
     1561 cgctggaact cctttcccgc tttgtactct gcacggaaat aattttcatg atgcatgcta
     1621 tccggtttcc cgtcaccttc acgcaaagcg tgatcgttga atcgatccag tcgatggtgg
     1681 gaaacctctt ctttttcatg ccgatgcagc taggtgcccg ggaaggggga ttcatcctgg
     1741 ttttcggcat cttgtcgctt cccgctgcac atgcggttta cgtgagctta tgcatccgta
     1801 ttcgcgaact tttctggacc ctgtttggat tggggttaat ccaggtgagg agaaggtagg
     1861 tagatcatct tcttcgtcat tccaccgtcg atcacgaagt tctgcccggt gatgaacccg
     1921 ttggccgaat cgagcaggaa catcaccatc tgtgcgatat caaaagcgtt ccccacacgt
     1981 cctgccgggt gctgtgcatg atcggattcc gacaattcta cctgccgggc tttcgctttt
     2041 ttcttcacgg ccgaaacatc tatccatccc gggctgatgc tgtttacccg aacgttggga
     2101 gccaggctca tggccagggc atgggtgagc gaatagattc cgcctttgct ggcggagtag
     2161 gactcggtat ccggttcgga ctgaaaggca cgcgtagaac acatgttcac catgcttccg
     2221 cgcgattcaa tcaggtgagg agaagagaac ttggcgcaga ggaacgttcc tgtcaggttg
     2281 gtgccgatgg cacggttcca ctcctccagc gtcagttcac tcatgggctt gaagacctcg
     2341 taaaccgcgt tgtttaccaa tccgtagatc ccgtcaaact ttttcaggga tgcccttacc
     2401 gaagacctca cgctcacctc actggatacg tcggtaataa aaaaagcgga tcgtttttta
     2461 tcgaattgcg gttttatctc ctccatcgct tcattatcca tatcgaaaat ggagacagcg
     2521 taccccactt taagcagtga ctgcgcgatg atgcgcccga tgccctgagc acctccggtt
     2581 acaataacgt gtttcataga gccctggttt tttgttgagt ttccaaaagt agtgattttt
     2641 tacgaaatac gtaaatccgt aatttctact tatctttgta acgctaaaaa taaagaagca
     2701 atgaatcaag aacacctgct gggtatgacg ctttccgaaa tccaaaacat cattttggag
     2761 ctcggcctgc cgagatttac cgccaaacaa attaccgact gggtttatgt aaaacgcgtg
     2821 aagagtatcg acgaaatgac caatatttcc gtacggaa
//
LOCUS       DAOSDR010000396         2845 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrimonas sp. isolate AMR_MDS_1826
            NODE_12399_length_2845_cov_2.130108, whole genome shotgun sequence.
ACCESSION   DAOSDR010000396 DAOSDR010000000
VERSION     DAOSDR010000396.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863355
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrimonas sp. (wastewater metagenome)
  ORGANISM  Petrimonas sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Dysgonomonadaceae; Petrimonas.
REFERENCE   1  (bases 1 to 2845)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2845)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:10:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,720
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 35
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2845
                     /organism="Petrimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12399_length_2845_cov_2.130108"
                     /isolate="AMR_MDS_1826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2023866"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            26..>2845
                     /locus_tag="PLI30_12440"
     CDS             26..>2845
                     /locus_tag="PLI30_12440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019538946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0071702 - organic substance transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SusC/RagA family TonB-linked outer membrane
                     protein"
                     /protein_id="HOI80398.1"
                     /translation="MKRKQRADAPGVFSKIVKRLLILMLLLNSGLQAKASSDLVSQQP
                     ERTVSGVVTDENGDSVIGATVVVKGTTIGTTTNVDGIFTLRIPSDAQTLQVSYVGMIT
                     VDITIGDNSQYNVVMKPDAVLMEDLVVIGYGTRSKKDMSIAVSSVKSDELNERPSAFN
                     IMQGIAGKVAGVTNVSMSGRPGGSSSLRVRGMGSINAGKDPIYVLDGVIGVDPNIINS
                     ANIESIDILKDAAATAMFGAQGSNGVVLINTKKGKKGKGSITYDGKMGFGFMNRKLDM
                     LNSDEYMEVQRRSYAYSGQTMPHLTTADEKLFYYSKDASGNYQYDENGLLIASPKYNT
                     DWQKEMTRTAITNDHIVSFSSGNEDTSVYSSVAYQNYEGVMKSSEYERFSGTVNVNSR
                     IKDWLRVQVVGTVGSQKGNNNDMESSFGQGAIRNMIEMPPIVPVKYEDGTWGRKGDYK
                     FSEEGENPLLLLRDRRDEWKSNFSVFSLNSILDLTRKLTLTVQGDYQQSNRKGMSYAK
                     AGLLDVSENNGGYADINNSDNQKLTSENYFTYTDEYFNGQLSSNFVLGASWYYNRNES
                     SSSGSEQYFDDSFDYHNLSAGTTWHEPRSGMNQNTMNSYYFRMNHSLRDRYLLGVTFR
                     ADGASNFGMNNKYGYFPSVSAGWRISEESFFQPLTNVVSQAKLRTSYGVVGNASIPNY
                     RTISQYGNGSTIFNKELTSYVTLANLGNKDLKWESSRQFNIGLDLSFLKNRLEVIMDY
                     YTKSTRDLLFEKQIPITTGYATTWSNLGEIRNDGFEATITSRNIHTKDFMWATDLVYS
                     TNKVKTIDINGETIETGNNTIAREGIDWASYYVYKRLGTWSLAEVEEAARYGKRPGDI
                     KYEDVNGDYKIDEEDRQLMGTGRPKGNLTMVNTFTYKGLSLMVDLNYVYGFKIMSIAH
                     SMGENRPLYSNSVKDILKAWTPENQN"
BASE COUNT          888 a          566 c          664 g          727 t
ORIGIN      
        1 tattaatcaa aaattgttta caaacatgaa aagaaaacag agagcggatg cacctggtgt
       61 gttttccaaa attgtaaaac ggctgttgat cctgatgctg ctattaaata gcggattaca
      121 agcaaaagcc tcttcagatc tcgtttctca acaacctgaa agaactgttt ctggtgtggt
      181 gacggatgag aacggtgatt ctgtaattgg tgcaactgtt gttgtaaaag gtacaacaat
      241 aggtactact acgaatgttg acggaatttt tactttaaga ataccttcag atgctcaaac
      301 gctgcaggtt tcgtatgtgg gtatgataac tgtagatata accatcggcg ataattcaca
      361 gtacaatgtg gttatgaagc cggatgctgt tctgatggaa gatctagtag ttatcggata
      421 cggaacccgc tcgaaaaaag atatgtccat agctgtgagt tctgtaaaaa gtgatgaatt
      481 gaatgaacgc ccgtctgcct ttaatatcat gcagggtata gccggaaaag tggctggggt
      541 cacaaacgta tcaatgtctg ggaggccagg cggttcatct tccctccgcg ttcgtggtat
      601 gggttccatc aatgcaggta aagaccctat ctatgtactc gatggtgtaa ttggggttga
      661 tcccaatatt atcaactctg caaatattga atctatagac attcttaagg atgctgctgc
      721 aacagctatg tttggcgctc aaggatcaaa cggagttgtg cttattaaca ccaagaaagg
      781 taaaaaagga aaaggctcca ttacctacga tggaaagatg ggatttggct ttatgaatcg
      841 aaaactagac atgcttaatt ccgatgaata tatggaagtt cagcggcgca gctacgctta
      901 cagcggacaa accatgcctc accttactac ggcggacgaa aaactcttct actattctaa
      961 agatgcttcc ggtaactacc aatacgatga gaacggtctg ctgattgctt cgcccaagta
     1021 caatacagac tggcagaagg aaatgactcg cacggctatt actaatgacc acattgtatc
     1081 tttttcatcg ggcaacgagg ataccagtgt ttattcgagt gttgcttacc aaaattatga
     1141 aggagtgatg aaaagttccg aatacgaacg tttctcgggt actgttaatg taaacagcag
     1201 gataaaggac tggcttcgcg tgcaggttgt aggaaccgta ggtagtcaga aaggcaacaa
     1261 taacgatatg gagagttctt tcggtcaagg agcgatccgt aatatgatag aaatgccgcc
     1321 cattgtaccc gttaagtacg aagacggcac ctgggggcgc aagggggatt ataaattttc
     1381 cgaagaagga gagaatccat tgttgctgtt acgagacagg agagatgagt ggaaatccaa
     1441 cttctccgta ttcagcctca attccattct cgacctaacc agaaagctaa cgttgactgt
     1501 ccagggagac tatcagcaga gcaatagaaa ggggatgagt tatgccaaag ccggacttct
     1561 ggatgtaagt gaaaacaatg gtgggtacgc ggatatcaac aactccgata atcagaagct
     1621 taccagtgaa aactacttta cctataccga cgagtatttt aacggacaac tcagctctaa
     1681 ctttgtattg ggtgccagtt ggtattataa tcgcaatgaa agttcatcct cgggttccga
     1741 acaatacttc gatgattcgt ttgattatca caacctttcg gcaggaacaa catggcacga
     1801 acccagatca ggaatgaatc agaatacgat gaactcatac tatttccgga tgaatcacag
     1861 tctccgggac aggtatctgc tgggtgttac attccgtgcg gatggtgcct caaactttgg
     1921 tatgaacaac aagtacggat atttcccatc agtatctgcc ggatggcgta tttcggaaga
     1981 gagttttttc caacctctca ccaatgtagt aagccaggcc aagctgagaa caagttacgg
     2041 cgtagttggt aatgcttcca tccccaatta cagaaccatt tcacaatacg ggaacggatc
     2101 taccatcttc aacaaggagc tcacgtcgta tgtaactttg gccaatttgg gtaataaaga
     2161 cctcaagtgg gaatcatctc gccagtttaa tattggatta gacctaagct tcttaaaaaa
     2221 ccgtttggag gtaatcatgg actattacac caaatcaacc cgagacttgt tattcgagaa
     2281 acagatacct atcaccaccg gatatgctac tacatggtct aaccttggcg agatacgaaa
     2341 tgacggcttc gaggctacca ttacatcacg caacattcat acaaaagatt tcatgtgggc
     2401 gacagatctt gtgtactcca ctaacaaagt gaagacaata gatataaacg gagaaaccat
     2461 tgagacaggg aataatacca tagcccggga aggcatagac tgggcatcgt attacgtgta
     2521 taaaagactg ggaacatgga gtcttgccga ggtagaggaa gctgccaggt atggtaaaag
     2581 acccggtgat atcaagtacg aagatgtgaa cggtgactat aagatcgacg aggaagatcg
     2641 tcagttaatg ggtacaggca ggccgaaagg aaacctcacc atggtgaata cttttaccta
     2701 caagggttta tcactgatgg ttgaccttaa ttatgtatat ggcttcaaga tcatgagtat
     2761 tgcccattcc atgggtgaaa acagaccgtt gtacagcaac agtgtgaaag atatacttaa
     2821 agcatggaca cccgagaatc aaaat
//
LOCUS       DAOSDR010000397         2842 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrimonas sp. isolate AMR_MDS_1826
            NODE_12406_length_2842_cov_4.783280, whole genome shotgun sequence.
ACCESSION   DAOSDR010000397 DAOSDR010000000
VERSION     DAOSDR010000397.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863355
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrimonas sp. (wastewater metagenome)
  ORGANISM  Petrimonas sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Dysgonomonadaceae; Petrimonas.
REFERENCE   1  (bases 1 to 2842)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2842)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:10:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,720
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 35
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2842
                     /organism="Petrimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12406_length_2842_cov_4.783280"
                     /isolate="AMR_MDS_1826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2023866"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1019)
                     /gene="cysS"
                     /locus_tag="PLI30_12445"
     CDS             complement(<1..1019)
                     /gene="cysS"
                     /locus_tag="PLI30_12445"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018964514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HOI80399.1"
                     /translation="MSQPLVIYNTLSRAKELFEPIKPPFVGMYVCGPTVYSDVHLGNC
                     RTFISFDLIYRYLIHIGYKVRYVRNITDAGHLEGDNDEGEDKFAKKAKLEQLEPMEIV
                     QKYTLGFHEILRIFNTLPPSIEPTATGHIVEQIEMIKEILAAGYAYETAGTVYFDVEK
                     YSRKYSYGILTNRKLEDLLEGTRELSGQDEKKGRLDFALWIKAKPETLMKWPSPWGWG
                     FPGWHIECSAMGTKYLGTTFDIHGGGMDLQATHHTNEIAQSQACHHAEPVKYWVHTNM
                     LTVNGARMSKTAGNGFLPKELFTGDHPLLDRGYSPMTVRFFMAQSHYRSTLDFSNEAL
                     QAAEKG"
     gene            1197..1898
                     /locus_tag="PLI30_12450"
     CDS             1197..1898
                     /locus_tag="PLI30_12450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006801289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5020 family protein"
                     /protein_id="HOI80400.1"
                     /translation="MLRKLFLFTLSLVAFASLNAQNLQLHFDPRHAFYGDDVAAKNYL
                     TATFEMFKPDAWGSTFMFVDFDFNGDKRNIGLVYAEIARAFKIKDFALMPHLEYNGGL
                     GTGFSIPSSYLAGAQYPFKLGNFFMGTYLAYKLNAFTDLSHDVQWTLTWNSAFPNSKV
                     SLAGFLDLWTENKNRATGEGGKKLILLSEPQVWYNFTPNFAIGSEIELSYNFVNAFAE
                     SKFYAVPTLATKWNF"
     gene            1981..>2842
                     /locus_tag="PLI30_12455"
     CDS             1981..>2842
                     /locus_tag="PLI30_12455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019539413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HOI80401.1"
                     /translation="MIEKLFKLKENNTNIRTEILAGIITFLTMSYILAVNPQILGETG
                     MDKGALFTTTAVAAIAGTIFMALIANVPIAQAPGMGLNNFFAFSVVIAMGHSWQFALT
                     GVLLSGFCFMLLTIFNIRKIIVDNIPVALKNAIPIGIGLFITLIGLKSAGIVTPNQFT
                     LVQLGNMADPNVWIAVLGLVVIAVLLVKKVHGAILIGIIISTIFAGFLGMIQLPQGSW
                     ITLPPSIEPIFFKFEWSSILTFDMLIVVFTFLMVNIFDAMGTLIGVISKIGKSEKDGS
                     FPQLQKALFAD"
BASE COUNT          753 a          647 c          589 g          853 t
ORIGIN      
        1 cctttttctg cagcctgcag cgcttcattg gaaaaatcaa gtgtactgcg atagtgcgat
       61 tgcgccataa aaaagcgtac ggtcattggt gaataccccc tgtctaataa ggggtggtcg
      121 cccgtaaaca attctttagg caaaaatccg tttccagctg tttttgacat tcgggcgccg
      181 ttaactgtca acatattggt gtgaacccag tattttaccg gttccgcatg atgacaagcc
      241 tgcgattggg caatttcatt ggtatggtgc gttgcctgca aatccatccc tcccccgtgg
      301 atgtcaaagg tagttcccaa atacttggtg cccatggcag aacactctat gtgccagcca
      361 ggaaatcccc aaccccacgg cgatggccac ttcatcaacg tctcgggttt tgccttgatc
      421 cagagcgcaa aatccagcct gcctttcttt tcgtcttgcc cgcttagttc ccgcgttcct
      481 tcgagcagat cttccagttt acggtttgtg agtatcccgt acgaatattt acggctgtat
      541 ttctccacat cgaaataaac cgttcccgct gtttcgtagg cataccctgc agcaaggatt
      601 tcttttatca tctctatttg ctccacgata tgtccggttg ccgtgggctc gatgctagga
      661 ggcagcgtat tgaaaatacg caatatctcg tggaaaccga gggtgtattt ttgtacaatc
      721 tccatcggct ccagttgttc taatttcgct tttttagcaa acttatcttc accctcatcg
      781 ttatcgcctt ccagatgtcc tgcatccgtg atgttcctca cgtacctcac cttgtatccg
      841 atatgaatca agtacctgta aataagatca aaagaaataa atgtacggca gttccccaaa
      901 tgcacatcgc tgtacaccgt aggtccgcaa acatacattc ccacaaaagg cggctttatg
      961 ggctcgaaaa gctcctttgc acgactgagc gtattgtaaa tgaccagagg ttgcgaaata
     1021 ttttcctgca taatagctaa cttgagtttg aattgaagag caaaaataca aatttatcca
     1081 ttaactgaag aagatatcaa acaaaagata aaaaagtagt attaacactt aaatggttta
     1141 cattttttta ttattttcgc cgtttctttt caataatctt taaattttaa ttatttatgt
     1201 tgcgaaaatt atttctgttt accttatcat tggtggcatt tgcctcgctg aacgcgcaaa
     1261 acctacaatt gcacttcgac ccgcgccatg cattttatgg tgatgatgtg gcagccaaaa
     1321 actaccttac tgcgactttc gaaatgttta aacccgatgc ttggggttca acattcatgt
     1381 tcgtagattt cgattttaat ggcgacaaac gaaacatagg attggtctat gccgaaattg
     1441 cccgggcatt caaaatcaag gatttcgcat taatgccaca tttggaatac aacggcggtt
     1501 tagggaccgg tttttcaatt cccagctctt accttgccgg tgctcagtat ccgtttaagc
     1561 tcggcaattt cttcatggga acgtaccttg cctataagct gaacgccttc accgatttaa
     1621 gccacgacgt acagtggaca ttgacctgga actcggcatt tccaaacagc aaagtatcgc
     1681 tggccggttt tctggatctg tggactgaaa acaaaaatcg ggctaccgga gaaggcggca
     1741 agaaattgat tcttttaagc gagcctcagg tatggtataa cttcacaccg aattttgcta
     1801 tcggtagcga aatagagttg agttataatt tcgttaacgc ttttgccgaa agcaaattct
     1861 acgctgttcc taccctggca accaaatgga acttctaatc gcttcgccta tgtaattgcc
     1921 taaaggcgaa tatcatttcg tcctggctgt gaatatcaac tgaattacaa acgaacaatc
     1981 atgatagaaa aactattcaa acttaaagaa aacaacacca acatccgcac agagatcttg
     2041 gccggtatca tcaccttttt gaccatgtcc tatattttgg ctgtgaaccc tcaaatattg
     2101 ggtgagacag gtatggataa aggagcgcta ttcacgacga ctgccgttgc ggctattgcc
     2161 ggaaccattt ttatggcact cattgccaat gtacctatag cccaggcacc tggaatgggg
     2221 ttgaacaact tttttgcttt cagcgtagtt attgcgatgg gacactcctg gcaatttgct
     2281 ttgacaggcg ttttactttc gggtttctgt ttcatgctgc tgaccatttt caatatccga
     2341 aaaattattg tagacaatat tccggtggcg ctaaaaaatg cgatccctat tggtattgga
     2401 ttgtttatca cgttgatagg actgaaaagt gcggggattg taaccccaaa ccagtttacg
     2461 ctcgttcaac tcgggaacat ggcagatccc aatgtttgga ttgcagtact cggattggtg
     2521 gttattgcag tgcttttggt taaaaaagta cacggtgcga tcctgatcgg aattattatt
     2581 tccaccatat ttgccggatt tttgggaatg atccagttac ctcaaggcag ctggattaca
     2641 cttccgccca gcatcgaacc tatctttttt aaattcgaat ggagcagtat cttaactttc
     2701 gacatgctga tcgttgtgtt tactttcctg atggtcaaca tctttgacgc tatgggcaca
     2761 ttgattggtg tcatctcaaa aatcggaaaa tcggaaaaag acggtagttt ccctcaatta
     2821 cagaaagcat tgtttgccga cg
//
LOCUS       DAOSDR010000398         2819 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrimonas sp. isolate AMR_MDS_1826
            NODE_12553_length_2819_cov_4.124819, whole genome shotgun sequence.
ACCESSION   DAOSDR010000398 DAOSDR010000000
VERSION     DAOSDR010000398.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863355
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrimonas sp. (wastewater metagenome)
  ORGANISM  Petrimonas sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Dysgonomonadaceae; Petrimonas.
REFERENCE   1  (bases 1 to 2819)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2819)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:10:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,720
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 35
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2819
                     /organism="Petrimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12553_length_2819_cov_4.124819"
                     /isolate="AMR_MDS_1826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2023866"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            94..2034
                     /locus_tag="PLI30_12460"
     CDS             94..2034
                     /locus_tag="PLI30_12460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005861612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amylo-alpha-1,6-glucosidase"
                     /protein_id="HOI80402.1"
                     /translation="MSYLKFDKDLMINLEYSLYRNILRTNRKGAYQNTSISGCNTSKY
                     QGLLVMPVPFLDDDNHLILSSIDETVIQHGAQFNLGIHKYNDDNYSPKGHKYIREYNI
                     DSVPKTIYRVGGVILSKEIMFSSKENRLMIRYKLMDAHSPTTMRFKPCMAFRNISQLT
                     RQNDQVDRSYREVENGISTSMYYGYPEFFMQFNKRVDFMYQPYWNQGVEYIQDMQNGD
                     TFKEDLYVPGYFEMPIAKGEEVIFSAGDILVNTSALTDEFNAELKKRTPRSSFYNCLK
                     NSAHQFYYIPKTDEMYLLAGYPWFKVRARDQFVSLPGCTLSVGRPDDFTKIMDSAIPH
                     IQDFLNDKPRKNIIKQIDDPDVLLWVTWALQQYWKENKPVFKEKYVDFLFSIIDFILS
                     NKHPNLTVDPENGLVSTYGRDVAVSWMNAVQNGKPVTPRSGYLVEFNALWHNALKFAE
                     EVAAATNKEVLATTYEEKALKAQQSFIETFLNDAGYLYDYVDGTYADRNVRPNMIFAV
                     SMDYSPLDRRQKKLVIDFVTKELLTPVGIRSLSPKGYNYRPRYAGTSEEKEYAYFNGC
                     AFPWLIGAYIEAYLKVFSMSGLSLADRVMIELEDQMQNDCIGTLSEFYDSSPPFYAHG
                     GYSFAMSVSETLRAKRLIRSFG"
     gene            2104..>2819
                     /locus_tag="PLI30_12465"
     CDS             2104..>2819
                     /locus_tag="PLI30_12465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019540303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HOI80403.1"
                     /translation="MKALMLGWEFPPHILGGLGTASYGLTKGMAKQEDMEILFVIPKP
                     WGDEDQSFLKIIGCNNTPVVWRDVSWETAKARLEKYMNPEEYFWMRDNIYANFNYMNT
                     NDLGCLEFSGRYPNNILEETNNYSIVTGVIARTYEYDIIHAHDWLTYSSGLHAKSVSG
                     KPLVIHVHATEFDRSRGKPNPTVYGIEKNGMDHCDHIICVSNLTRKTVIENYHQPEWK
                     VSTVHNAVEPLSPEIEAIER"
BASE COUNT          840 a          625 c          633 g          721 t
ORIGIN      
        1 aaaaaagagt ttattatttg ccgaatcaac ggcaattaac tatctttcga acatcaaaac
       61 taaagtggta tatttctaat tcctctatca actatgagtt atcttaaatt cgataaggac
      121 ctgatgatta acttggagta ctcactgtat cgcaacatac tgaggactaa cagaaaaggg
      181 gcttatcaaa acacatcgat ctcgggttgc aatacatcga aatatcaagg acttctggtg
      241 atgccggtac cctttctgga cgacgacaat cacctgatcc tctcttctat tgatgaaacc
      301 gttatacagc acggtgcaca attcaattta ggaatccaca aatacaacga cgacaactac
      361 agcccgaaag ggcacaaata catccgtgaa tataatatcg atagtgttcc aaaaactatt
      421 taccgtgtag gaggcgttat tctttctaaa gagattatgt tttcatcgaa agaaaaccgg
      481 ctcatgatcc ggtacaagtt gatggatgcc cattcgccga ccaccatgcg gttcaaaccc
      541 tgtatggcat ttcggaacat ttcacagtta accaggcaaa acgatcaggt ggaccgttca
      601 taccgcgaag tggagaatgg aatcagcacc agtatgtatt atggttatcc cgaatttttc
      661 atgcaattca acaagagagt cgactttatg taccaaccct actggaatca gggcgtggag
      721 tacattcaag atatgcaaaa cggagatacg ttcaaggagg acctgtatgt ccccgggtat
      781 tttgagatgc caattgcaaa aggcgaggag gtaatttttt ctgctggtga cattctggta
      841 aacacctctg cccttaccga tgaatttaac gctgaactta aaaaacgcac gccaaggtct
      901 agtttttaca attgcctgaa aaactccgcc catcagtttt attacattcc aaaaacggac
      961 gagatgtact tgctggccgg ctatccctgg ttcaaggtac gcgcccggga tcagtttgtg
     1021 tcgttgcccg gttgcaccct gtcggtgggc aggcccgatg actttacaaa aatcatggat
     1081 tcggccatac cccatattca ggattttttg aacgacaaac cgcggaaaaa cattataaaa
     1141 cagatagacg accctgatgt cttgttgtgg gtaacctggg cactgcaaca atactggaaa
     1201 gaaaacaagc ccgtttttaa agaaaaatat gtcgactttc ttttttccat aatcgatttt
     1261 attttgtcaa acaagcatcc caacttaact gttgaccctg agaacggatt agtctcaacg
     1321 tacggacgtg atgttgccgt gagctggatg aatgcggtgc aaaacggcaa gcctgtcact
     1381 ccaagatcgg gatacctggt tgaattcaat gctttgtggc acaatgcact taaatttgcg
     1441 gaagaggtgg ccgctgcgac aaataaagaa gttctggcta caacatacga agagaaagcc
     1501 ttgaaggcgc aacagtcgtt tatcgaaaca tttctcaacg atgccggcta tttatatgat
     1561 tatgtggatg gcacttatgc agacaggaat gttcgtccca atatgatttt tgctgtttct
     1621 atggactatt cgccgctgga caggcgtcag aaaaaattgg tgatcgattt cgtcaccaaa
     1681 gagttgctta ccccggtggg catacgttct ttaagcccga aaggctataa ctaccgccct
     1741 cgttatgcag gaacatccga agaaaaggaa tatgcctatt tcaacgggtg tgcttttccc
     1801 tggcttatag gagcttacat tgaagcgtac ctgaaagtgt tcagcatgag cggactctcc
     1861 ctggcagacc gggtaatgat tgagctggaa gatcagatgc aaaacgattg cattggaacc
     1921 ttgtctgaat tttacgattc cagccccccg ttttatgccc acggaggata ttcttttgcc
     1981 atgagtgttt cagaaacttt acgtgcaaag agactcatca gatcatttgg gtaaaccttg
     2041 cggaaaacga tgaatcgtaa atgaagaact attgaactac aaaagaatat aaaacacgat
     2101 agaatgaaag cgttgatgtt agggtgggaa tttccgcctc atatactggg tgggcttgga
     2161 accgcaagtt atggattaac caagggtatg gctaagcagg aagatatgga aatcctgttt
     2221 gtcattccca agccctgggg tgacgaagac caaagttttc tgaaaataat tggctgtaac
     2281 aatactcccg tcgtatggcg cgacgtgagc tgggaaactg ccaaggccag gctcgagaaa
     2341 tatatgaacc ccgaggaata tttttggatg cgtgataaca tttacgctaa cttcaactat
     2401 atgaacacca acgacctggg atgtctggaa ttctcaggaa ggtacccgaa caatatcctg
     2461 gaggagacca acaattactc catcgttacg ggtgttattg cccggacgta tgaatacgac
     2521 attatccatg cgcacgattg gctcacctat tcctctggcc tccacgcaaa aagcgtaagt
     2581 ggaaaaccgc tggtcattca tgtgcacgca acggagttcg accgcagccg cggcaagcct
     2641 aatcctactg tttacggaat cgagaagaac ggaatggatc actgcgacca cattatctgt
     2701 gtaagcaacc ttacccgcaa aaccgtcatt gagaactatc atcaacccga atggaaagta
     2761 tccaccgtcc acaacgccgt tgagccgttg agcccggaga ttgaagcgat cgaacggat
//
LOCUS       DAOSDR010000399         2780 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrimonas sp. isolate AMR_MDS_1826
            NODE_12776_length_2780_cov_3.886606, whole genome shotgun sequence.
ACCESSION   DAOSDR010000399 DAOSDR010000000
VERSION     DAOSDR010000399.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863355
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrimonas sp. (wastewater metagenome)
  ORGANISM  Petrimonas sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Dysgonomonadaceae; Petrimonas.
REFERENCE   1  (bases 1 to 2780)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2780)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:10:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,720
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 35
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2780
                     /organism="Petrimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12776_length_2780_cov_3.886606"
                     /isolate="AMR_MDS_1826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2023866"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..564)
                     /locus_tag="PLI30_12470"
     CDS             complement(<1..564)
                     /locus_tag="PLI30_12470"
                     /inference="COORDINATES: protein motif:HMM:NF015382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carcinine hydrolase/isopenicillin-N
                     N-acyltransferase family protein"
                     /protein_id="HOI80404.1"
                     /translation="MRKLAILLLWIFLTLSEKSIPCTTAVISGKATPDGRSMIWKLRD
                     TDDLENCFRYFNDGEYSYIGLVNSGDMKGENVWGGSNSAGFAIMNSASYNVNENDTTS
                     LKDREGIFMKLALQTCASLQDFEKLLNEYPKPRGSAAHFGVIDARGGAVFYEVNNYTW
                     TKFDANAASEGYVIRTNYSETGTPDSGY"
     gene            complement(688..885)
                     /locus_tag="PLI30_12475"
     CDS             complement(688..885)
                     /locus_tag="PLI30_12475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005854813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HOI80405.1"
                     /translation="MIEKIGTNAGKVWTLLDEAGTQNVKDLKKSSKLTDKDLYAALGW
                     LAREGKVALVEDGKELFVSLI"
     gene            complement(1086..1673)
                     /gene="coaE"
                     /locus_tag="PLI30_12480"
     CDS             complement(1086..1673)
                     /gene="coaE"
                     /locus_tag="PLI30_12480"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006799550.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HOI80406.1"
                     /translation="MIKLGVTGGIGSGKSVVCDVFRLHDIPVFDADREAKKLNDTSPV
                     IREKLINNFGPDLYQGNKLDRKKLANLIFNNAENLRITNAIIHPELAKYFEKWVDQRK
                     HHAVIAIDAAVLYEAGFQSLLDKTIVVMAPLETRIERAAKRDRLSKEQITARANSQMN
                     DEEKAGLADFIIHNDGRHSLLEQVAQILQDISTLY"
     gene            complement(1675..2658)
                     /locus_tag="PLI30_12485"
     CDS             complement(1675..2658)
                     /locus_tag="PLI30_12485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="YbbR-like domain-containing protein"
                     /protein_id="HOI80407.1"
                     /translation="MDLASTKNRWIAKIRSFFSRLNWKNILLFFGFFILAFIFWLMLF
                     FQRDIEATYKIPLKYTNIPDDVVFDAVLPDDIEIRVADKGSEIFRYTFTLKDSIEINI
                     AKYKEERINNLQGTELMQLIRKKLSQSTNLKAYYPINISLVTSKLQKKELKVVFDGVI
                     ATSRSNLVADSATFLPGTVIAYGSQSQLAEISSAVTEYTLFNNLKATSQLKVKIMPVS
                     GVKFIPDEVELYIPVLEYTERKFEVPVTARNVPDNIDVKFFPSQTEVSFSVTLEEYKK
                     IAPEDFEVELDYKKFFKNEDGRVDLELSASPSPIRNVKLSPSSVEFLLESR"
BASE COUNT          731 a          589 c          579 g          881 t
ORIGIN      
        1 gtatcctgaa tcaggagtgc cggtttcgga ataattggtg cggatcacgt aaccttcgga
       61 agcggcattg gcatcaaatt ttgtccaggt gtaattgttc acctcgtaaa agaccgctcc
      121 gcccctggca tctataacgc cgaagtgtgc cgccgatcct cgcggctttg ggtattcatt
      181 gagcagtttt tcgaaatcct gcagcgatgc acatgtttgc aaagcgagtt tcatgaaaat
      241 accttccctg tctttcaagg aggtggtatc gttttcgttc acgttatacg atgcactgtt
      301 cataatggca aatccagcac tattggatcc gccccacacg ttttcacctt tcatatcccc
      361 ggagtttacc agcccgatat aactgtattc tccgtcattg aaatagcgga aacagttttc
      421 caggtcatcc gtatcacgca gtttccatat catgcttcgc ccatcaggcg tggctttccc
      481 ggaaataaca gccgttgtgc agggaatgga tttttcggag agggtgagaa atatccataa
      541 aagaaggatg gccaactttc tcattcgctt attttttagt aaagatagcg gataaaaaca
      601 gatcgtcgaa aaaaatggat acaaaaaagc cgtctttaaa gacggctttt actttatttt
      661 tttcttaaat cgttggttgt gtgattgtta tatcagcgaa acgaataatt cttttccgtc
      721 ttccaccaac gctacttttc cttctctggc taaccagcca agagcagcat aaagatcttt
      781 atccgtcaat tttgaagact tcttcagatc ttttacgttt tgtgttccgg cctcgtccag
      841 caatgtccag actttaccgg cattggtccc aattttttct atcatttttt taattttaaa
      901 taagtaaatt acatttcgca tgtatttata acgaatacgt tcagtgcgaa agtccactag
      961 ataacctaaa tccgatacaa aagtagtgaa tttatgttta atgtctttta ttgatagtat
     1021 aaaaatactt aattaagtgg ttgattagta cgtttttcta caggaagccc tccctgaagc
     1081 ctgtattaat aaagggttga aatatcttgt aggatttggg caacctgttc caaaagggaa
     1141 tgccgtccat cgttatggat gataaagtcg gccagtcctg ctttttcttc gtcattcatt
     1201 tgactgttgg cgcgtgccgt tatttgttct ttggagagac ggtccctttt tgccgctctt
     1261 tcaatgcgtg tttccaaagg agccattacg actatggtct tatccagcaa cgattggaat
     1321 cctgcttcgt acaaaacggc tgcatcaatg gcaataacag catggtgttt tcgttgatcc
     1381 acccattttt cgaaatattt tgccagctcg ggatgtataa tagcgtttgt tatacgaaga
     1441 ttttctgcat tattgaaaat aaggtttgcc agttttttgc ggtcgagctt gttaccctgg
     1501 taaaggtctg ggccgaagtt gttgatcagt ttttcacgga ttacgggaga ggtgtcgttc
     1561 agctttttgg cttcccggtc ggcatcaaaa acagggatat cgtgaagtcg gaacacatcg
     1621 cagacaaccg attttccact tccgatccca ccagtaactc ctagctttat catcttatct
     1681 gctttctaaa aggaactcta ccgacgaggg cgatagcttg acgttacgta tgggcgaagg
     1741 gcttgccgat aattccaggt ctacacgtcc atcctcgttt ttgaagaatt ttttgtaatc
     1801 cagttcaacc tcaaaatcct ctggggcaat tttcttgtat tcttccagag ttaccgagaa
     1861 cgatacttct gtttgcgatg ggaaaaactt cacgtcgata ttatcaggaa catttcttgc
     1921 cgtgacggga acttcaaatt ttctttccgt atactccaaa acggggatat aaagttctac
     1981 ctcgtcgggt atgaacttga ctccgctcac aggcataatt tttactttca gttgcgaggt
     2041 agctttcagg ttgttgaaca gggtgtactc ggttacagct gacgaaattt cagctaactg
     2101 gctttgagaa ccgtaagcta ttacggttcc gggcagaaat gtagcgctgt ctgccaccag
     2161 attactgcgg ctggtagcga taaccccgtc gaaaactacc ttcagttctt tcttttgaag
     2221 cttagaggta accagcgata tgtttatggg gtagtaggct ttgaggtttg tgctctggga
     2281 taattttttc cggatgagtt gcattagttc agtcccctgc aggttattga tccgttcctc
     2341 tttgtacttg gcgatattaa tttcaataga atccttcaag gtaaaggtgt atcggaatat
     2401 ttccgaacct ttgtcggcaa ctcgtatttc aatatcgtca ggcaatacag catcaaaaac
     2461 cacatcgtca gggatatttg tgtacttcag cggtatttta taggtagcct caatgtcgcg
     2521 ttggaaaaat aacatcaacc aaaaaataaa agccagaatg aagaatccga aaaaaagaag
     2581 aatgttcttc cagtttaatc tggaaaagaa cgatcgtatc ttcgcaatcc atctattctt
     2641 ggttgatgcc aaatccatag ttaaacaaac ggtttcttaa ggtgtagtca catcggatga
     2701 agaggcaaaa accgatgttt tttcaaattt taattttaca ccatcggcta cctctaccag
     2761 aaaccaggtt tcgccaattt
//
LOCUS       DAOSDR010000400         2776 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrimonas sp. isolate AMR_MDS_1826
            NODE_12793_length_2776_cov_3.317898, whole genome shotgun sequence.
ACCESSION   DAOSDR010000400 DAOSDR010000000
VERSION     DAOSDR010000400.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863355
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrimonas sp. (wastewater metagenome)
  ORGANISM  Petrimonas sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Dysgonomonadaceae; Petrimonas.
REFERENCE   1  (bases 1 to 2776)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2776)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:10:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,720
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 35
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2776
                     /organism="Petrimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12793_length_2776_cov_3.317898"
                     /isolate="AMR_MDS_1826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2023866"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1018)
                     /locus_tag="PLI30_12490"
     CDS             complement(<1..1018)
                     /locus_tag="PLI30_12490"
                     /inference="COORDINATES: protein motif:HMM:NF021665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="G8 domain-containing protein"
                     /protein_id="HOI80408.1"
                     /translation="MTYRTVLRSIMLVVGILLSYNLTGSKSRGESILFVPSPNIFNVN
                     DNSRFELYFSSPWLYHTNPESSKLLDSYTAITSGDWNTPSTWGGVVPTVNDDVTIPTG
                     VTITVNTPSFCNNIVINGTLSITGNNRLQVNGNWENYGTFTPGTNGTIEFAGSSNISI
                     NGNTNFEELIINKGNLNNTITINGTSTITSGGSLAFNSGRIIIPPAGSFSINPSNGIT
                     IPALAGIEVTGGTLNTGHFTITNEGLIRISSGTAIFGTNSGNSVHTQGKGAFIVNGGS
                     VNIAGRLESSAAGILNPPNVPSGISISDGTVTLCTVGNGLNNTGSLNITSNGDFSFTG
                     GTIIF"
     gene            1960..2715
                     /locus_tag="PLI30_12495"
     CDS             1960..2715
                     /locus_tag="PLI30_12495"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HOI80409.1"
                     /translation="MMNFSRRKFLQRTAVTASAMAVIPEIIAAALPAEKRQAVSIPKN
                     GVILFQGDSITDAGREKTKELPNNGASFGTGYAFVAATSLLERFAGQQLSVYNRGISG
                     NKVFQLADRWQKDCLDLKPDVLSILIGINDYWHMRNGNYDGTVEIYEADYRKLLQQTK
                     AALPNVQLVICQPFALPGTTAVDESWVAPVKAYQEVAEKMSREFNATWVPFQRVFDEA
                     IRRAPAKYWAADGVHPSMAGCQLMAAAWLKAVL"
BASE COUNT          709 a          563 c          645 g          859 t
ORIGIN      
        1 gaaaaataat agttccacct gtaaaactga aatcgccgtt tgaggttatg tttagcgatc
       61 ccgtgttgtt taaaccattg cccacggtac aaagtgtaac tgttccgtct gaaattgaaa
      121 ttcccgaggg gacgtttggg ggattaagta ttcctgctgc tgaactttca agtcttccgg
      181 caatgtttac cgatcctccg ttaacgataa aagctccttt gccctgggta tgcaccgaat
      241 ttcctgaatt ggtgccaaaa attgcagttc cagaggaaat acggatcaac ccttcgttgg
      301 tgattgtgaa atggccggtg tttaacgtgc ctcccgtaac ctcgatgcct gcaagcgctg
      361 gtattgttat gccgttgctt ggattgattg agaagctgcc tgctggtggt ataatgatcc
      421 ttccactgtt aaatgccaga ctgcccccgc tggttatcgt actggtgccg tttatggtga
      481 tggtgttatt caggttgcct ttgttgatta ttaactcttc gaagtttgta ttgccattga
      541 ttgagatgtt tgaagagccg gcaaattcaa ttgttccatt tgtaccggga gtaaaagtgc
      601 cataattttc ccagttgccg tttacctgta aacggttgtt gcctgtgata cttaatgttc
      661 cgttaattac aatattgttg caaaaggagg gggtgttgac ggtaatagta actcctgtcg
      721 gaatggtgac atcgtcgttt acagtcggaa caactcctcc ccaggttgaa ggggtattcc
      781 agtcgccgct ggttattgca gtgtagctgt ccagcagttt tgaagattca ggattagtat
      841 gataaagcca gggagaagaa aaatataatt caaaccggga attgtcgttt acattaaaaa
      901 tatttgggga tggaacaaaa aggatgcttt ccccccgaga tttgctccct gtcaggttgt
      961 atgaaaggag aatacctact accagcataa tgctcctcag cacggttcga tatgtcacac
     1021 ctttgcccat ggtgaattgc gaaaggggaa attttaacgg ggctacaatg ttttcaggag
     1081 gccgtaatcc ggctcattaa ctgatgttga agtttttgat gtaaaatttt atcgctacct
     1141 cttttttctg aattagacct cttcttgccc acatgtcaac ctaacccaat ttgccgccgc
     1201 aacttttagt ttaaatcaac aataatatac ctgccctttt atgtctttat ttaaaattat
     1261 cacgggtgcc tctcgcacct gaaaaacttt tccggtgttt tgcccttacc agaaaagctc
     1321 atccttacaa atttactgga attatttgtg atataacagc ttgcatctgt tgaaatattt
     1381 tccggagata atgggtgaaa tgggggttgg ttgtcttgta agtttttgta tttcagatgc
     1441 ttgtttgtgt tttgggtgga tttttcaagc taagtcggtt aatcgtttat tacagagaaa
     1501 ttattaacat atcttttttg gaatgatctt ttgggtctta aatctgttga aaactgcaag
     1561 ttgcagcctt tttctacata attataaaaa tgaacaagca tctgtttttt gaaaaacaga
     1621 acaaaagctt aacagtcttt cactcgactt ttctccccgg aaattttcat ttttgcctgc
     1681 cggctaccaa agaacgattg tttttgtttt aggatttttg gtttgattat cagtgtatcc
     1741 aaagttaagt taactggctt gaaaatgcaa tagttgcact gtttaatttc tacgcagcca
     1801 tcgctatttt tgcagcaata aagggtgact tttcaaattt attttccgga aaccggaaga
     1861 ttcggtttca tttctaagaa tagctttctg ttttgctatc cggttttaaa ggggaacctg
     1921 tatatttaca ccaataagtc aacttaattt aaactgaata tgatgaactt ttcacgcagg
     1981 aaatttcttc aacgtactgc cgttactgca tcggccatgg ccgtgatccc ggagattatt
     2041 gccgctgcct tgccggccga aaaacgccag gcggtttcga tcccgaaaaa cggagttatt
     2101 ctgtttcagg gagactccat taccgacgca ggacgtgaaa aaaccaaaga acttcccaat
     2161 aatggtgctt cgtttggtac cggatatgct tttgtggcag ctaccagtct tctggagcgt
     2221 tttgccgggc agcaactttc ggtctataac cgcggaatca gtgggaacaa ggtgtttcag
     2281 ctggccgacc ggtggcagaa agattgcctc gacctgaaac ccgacgtgct gagcattttg
     2341 atcggaatca atgattactg gcacatgcga aacggaaatt atgacggaac ggttgagatc
     2401 tacgaagccg attaccggaa gttgttgcag caaaccaagg cggctctgcc caatgtacag
     2461 ctggttattt gccagccgtt tgctttaccc ggaaccactg cggttgacga gtcgtgggtg
     2521 gcgccggtaa aagcgtacca ggaagtggcc gagaagatga gccgggagtt taatgccacc
     2581 tgggttccgt ttcagcgggt gtttgacgaa gctatccggc gtgctccggc caaatattgg
     2641 gcagccgacg gcgtacatcc ctcgatggcg ggttgccagc tgatggcagc tgcctggctg
     2701 aaagccgttt tgtaagtggc gttttgacta gtcctaaaaa agcgttacag gattagacaa
     2761 aaataattta tttgat
//
LOCUS       DAOSDR010000401         2759 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrimonas sp. isolate AMR_MDS_1826
            NODE_12886_length_2759_cov_4.150888, whole genome shotgun sequence.
ACCESSION   DAOSDR010000401 DAOSDR010000000
VERSION     DAOSDR010000401.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863355
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrimonas sp. (wastewater metagenome)
  ORGANISM  Petrimonas sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Dysgonomonadaceae; Petrimonas.
REFERENCE   1  (bases 1 to 2759)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2759)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:10:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,720
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 35
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2759
                     /organism="Petrimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12886_length_2759_cov_4.150888"
                     /isolate="AMR_MDS_1826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2023866"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            426..>2759
                     /locus_tag="PLI30_12500"
     CDS             426..>2759
                     /locus_tag="PLI30_12500"
                     /inference="COORDINATES: protein motif:HMM:NF024974.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HOI80410.1"
                     /translation="MKQLALIFHLIHFSDIFLFVLHSPDFSLRKLFLKRVSPFTAITA
                     LLLLISLSLSAQKHGNYWYFGRNAGIKFTNGTPEVLFDGAIDNFEGVASISDYYGNLL
                     FYTDGITIYNRTHNVMENGNDLKGHPSSTQSGVIIPIPGDADRFIVFTVDYAYSGGDL
                     YYSVVDMKQAGGQGAVVTKNVFLQSNSAEKISAVSHSNHSDIWVVIHERESTEFKSYL
                     ITAAGVSSEVKSSFAGQILTSGVQGYLKFSTDGRYLAMATYHERRVDVFNFNPGTGDV
                     GFHSSYSFPDATYGVEFSGDSQFLYASVSYDLKQIHQINLGQKTSTLIASTGMAPGCL
                     QMAPDGKIYVARYNRPDVFSRYLGVIHNPNEEGTACNYVDEYLSLGANRGSLMGLPTF
                     IQSFFVPAVTLNGMNSCLEDTVHFSANLFGNLLLEHDWVLWDFGDPGSPDNTSDILYA
                     QHRFAAPGNYTITFIISSGGSELEASMEIEIYPMPQTDLPDTLKFCEGDSATLTAGEP
                     GYNYLWSNGLTSPAINVTASGKYWVRKMSEFGCSVSDTANIVTIPGPEIDLLGSYTIC
                     PDSVLVISAGDDQFTYQWIDMLTSSTRTIADPGFYSVKKTGTSGCYSEHSFLVDHFYV
                     PELSLGVDTLLCSPGGIDLIVSGFESYLWNDGSNSPVYHVDTPGIYSLVAFTADRCSA
                     ADSLMVSVCCEFSLVIPNVFTPNHDGINDTFKPVLHNAGRYKMTIANRWGVVLFETND
                     PEEGWDGYFMNERCPTGVYYVVFDIERCTPEGLFVNKK"
BASE COUNT          720 a          556 c          637 g          846 t
ORIGIN      
        1 tattcggctt tatggaacag agtatgaacg gacttactgt taaatctgta ggaataatga
       61 gagcaacagg aataatcggg ctaataaacc ttacctacaa tatatttaga tttgaacaag
      121 ttcagagact gaatttatgc aagagataac tccataataa tttgatagtt agccaattag
      181 ttaatttttt gcacgaaaaa taaaaatact tggaactttt gcacgtaaat tgaaatttta
      241 gttgtaatat tggcttactt tttcagcaca gatttgaatg ggttctattt tgtgtctgag
      301 aaaaataaaa aaatgaattt ttagaaccca cctttaccaa ctgtatacgg accggggtga
      361 catttttatg aattcattaa agcatgtttt aagaattgaa actttgtgta cgtgatataa
      421 tttctatgaa gcagttagca ctgatttttc atcttattca tttttctgac atttttttgt
      481 tcgtgctgca ttctcctgat ttttctctgc ggaaactgtt tctgaaaagg gttagtccgt
      541 ttacagctat tacagctttg cttttgctga tttccttatc gctgtctgca caaaagcatg
      601 ggaactactg gtattttggc cggaacgcgg ggataaaatt tacaaacggt acgcctgaag
      661 ttctttttga cggagctatc gataattttg aaggcgttgc aagtatcagc gattactacg
      721 gaaacctgct tttttatacc gatggaataa ccatttataa ccggacacat aatgtgatgg
      781 aaaatggtaa cgatttgaag gggcatcctt cgtcaactca gtcgggagta atcattccta
      841 ttcccggcga tgctgacagg tttatagttt ttaccgttga ttatgcttac tcgggcggag
      901 atctttacta ttcggtggtg gatatgaaac aggcaggggg gcagggagcc gtagtgacaa
      961 aaaatgtttt tctgcagtca aattctgcag aaaagatttc tgcagtcagt cactccaatc
     1021 attcagacat ctgggttgtg attcatgaac gggaatccac tgaattcaag agttatctga
     1081 taactgctgc cggggtaagt tctgaagtga aatcttcctt tgccggtcag atcctgacaa
     1141 gcggtgttca gggctatctt aagttttcaa ccgatggcag atatcttgcg atggctacct
     1201 accacgaacg tcgggtggat gttttcaatt ttaatcctgg aaccggggat gtgggtttcc
     1261 attcctccta ttcatttccg gatgccacat atggtgttga attttccggt gattctcaat
     1321 ttctttatgc ctcggtgtca tacgatctga aacaaattca tcagattaat ctcgggcaaa
     1381 agaccagcac actgattgcc tcaaccggga tggctccggg gtgtcttcaa atggctccgg
     1441 atggcaaaat ttacgttgcc cgttataacc gtcccgacgt attttccaga tatctgggtg
     1501 tgattcataa cccgaatgag gaggggactg cctgcaatta tgtggatgaa tatctgagtc
     1561 tgggagcgaa ccgtggttcg ctgatgggat taccaacgtt tatacaaagc ttttttgttc
     1621 ctgccgtcac ccttaacggg atgaattcct gtcttgaaga tacggttcat ttcagtgcaa
     1681 acctcttcgg aaacctgctg ctggaacacg attgggtcct ctgggatttt ggagatccgg
     1741 gttcacccga taatacatcc gatatccttt atgcgcaaca tcgtttcgcg gctccgggaa
     1801 attacaccat aacatttata atcagttccg gaggcagtga gctggaggct tctatggaaa
     1861 ttgaaatata tccaatgcca cagacagatc tgcccgatac gctgaaattt tgcgaaggag
     1921 attctgcaac gctgaccgcc ggtgaaccgg gttataatta tttgtggagc aatggcctta
     1981 caagcccggc aataaacgtt acggcttccg gtaaatactg ggtgcgaaaa atgtctgaat
     2041 tcgggtgctc ggtttccgat acggcaaata ttgttactat tccgggtcct gaaattgacc
     2101 ttctgggcag ctacaccatt tgccccgatt cggttcttgt tatatcggcc ggtgatgatc
     2161 agtttaccta ccaatggatc gatatgctga catcatccac acgcactatc gctgatccgg
     2221 gtttttattc tgtgaaaaaa accggtacat cggggtgtta ttccgagcac agctttttag
     2281 tggatcattt ctacgtaccc gagctttctc tcggagtcga taccttgctg tgctcacccg
     2341 gagggataga cctgatcgta tccgggtttg aaagctatct ctggaacgac ggaagtaatt
     2401 ccccggttta tcatgtggat actccgggaa tctacagtct tgttgcattc actgcagatc
     2461 ggtgttctgc agcggattcg cttatggtgt ccgtatgctg tgagttcagt ttagtaattc
     2521 ccaatgtgtt cactccaaac catgatggga tcaacgacac ctttaaacct gtgctgcaca
     2581 atgccggccg ttataagatg acgatagcaa atcgctgggg tgtggtactt ttcgaaacga
     2641 atgatccgga agaaggctgg gacggctatt ttatgaacga acgctgcccg acgggagtat
     2701 attacgttgt tttcgacatt gaaagatgta cgcctgaggg tttgtttgtc aataaaaag
//
LOCUS       DAOSDR010000402         2749 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrimonas sp. isolate AMR_MDS_1826
            NODE_12963_length_2749_cov_4.499258, whole genome shotgun sequence.
ACCESSION   DAOSDR010000402 DAOSDR010000000
VERSION     DAOSDR010000402.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863355
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrimonas sp. (wastewater metagenome)
  ORGANISM  Petrimonas sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Dysgonomonadaceae; Petrimonas.
REFERENCE   1  (bases 1 to 2749)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2749)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:10:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,720
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 35
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2749
                     /organism="Petrimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12963_length_2749_cov_4.499258"
                     /isolate="AMR_MDS_1826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2023866"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..191)
                     /locus_tag="PLI30_12505"
     CDS             complement(<1..191)
                     /locus_tag="PLI30_12505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005818165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HOI80411.1"
                     /translation="MNLNEFSRPNKRSNIVIPDVNGFKVLKCDFHMHTVFSDGDVWPT
                     VRVQEAWREGLDAIAITDH"
     gene            complement(439..2463)
                     /locus_tag="PLI30_12510"
     CDS             complement(439..2463)
                     /locus_tag="PLI30_12510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI80412.1"
                     /translation="MKKTGIGVLACLLLCLQGQAQLKVEDHRPLFVLKNDAIITAPEE
                     GVWSVATGWENDWMTEWIHATPTEVEQYDEWTILKGEIVLPHGVLYMRDAYREEKPGL
                     IKCVRRFEWKGEKTLSPLTLSVRLRVKGKEMSVFMPGILYYGNKNGARVNPDIIPVYT
                     GKAGEFAIFEDHRYPMPFVMLENREDQYAAAIHFTPSPVRGALLADQWWSAGVEAGDG
                     YTDFVLYSGPIGYNKKHSVAKALQLTPMKYTNTYLSMEPGRIIEKEFYIELYSIDREG
                     SGFQQPVYTSLDLHKPYDAERFPDFNTILASKYRFARSRWVDYGNNAAGYGMYDLQNR
                     KDMVRGWCGQAESPGYALQVLADRLNDEDLPAKVQQSLDFLASFPVNRENGMFPVGFN
                     GKEFYGGDHVSCGQALYNFAKAIETAQKNKRYNTEKWEAFLTSACDGQVKRILNPAWD
                     PHSTAEGFYMAPLAIASVLFGKKEYRQASEKIAAIYADRHLAMDGCYWGGTLDATCED
                     KEGAWAAFQGFLELYERFKEDKYLDWAKHAMDVCLSYVVVWDIPLPAGRLADFHFKST
                     GWSVVSPQNQHIDVYAVLFAPEVYKMGKYLDDDRLKQLARVMYRSCFQLTDMYGSQGE
                     QLQQTNFAQRGDMSNVHNLRGGYAEGWTVFWITAHFLNAAARFVELQVIP"
BASE COUNT          750 a          702 c          548 g          749 t
ORIGIN      
        1 atgtgatcgg ttattgcaat ggcgtcaagc ccttccctcc acgcttcctg aacacgtacc
       61 gtaggccaga catcgccatc ggaaaaaaca gtgtgcatgt ggaagtcaca cttcagaacc
      121 ttaaagccgt ttacatccgg tatgacgata ttactgcgtt tgttgggcct ggaaaattca
      181 ttgagattca tcacgccgtt attgatctgt tgggcctgcg aaacaaaggc gaaaaaagaa
      241 gcaagaaaca gggtaaaaat tgtctttttc atacaattat tttatttgtg ttatgtataa
      301 aattcagatg attccggata caaagaaacg aaaataaatc cggaaaaata ttacgttcta
      361 attaagaaac agcatttcca cactcacaca catgcgttct gacaaaaaaa accggataac
      421 cggaaaagac tcttgttttt aaggaataac ttgaagttcc acaaaacgag ccgccgcatt
      481 caggaaatgc gctgtaatcc agaaaacggt ccagccttct gcgtatccac cccgcaggtt
      541 atgtacattg ctcatatcgc cgcgctgcgc aaaattggtc tgttgcagtt gctctccctg
      601 cgacccgtac atatcagtaa gctgaaaaca cgagcggtac attactctcg ccaattgctt
      661 taaccggtcg tcgtccagat atttgcccat cttataaact tccggagcga ataggacggc
      721 atagacatca atatgctggt tttgcggaga gactacgctc caaccagtcg atttgaaatg
      781 aaagtcggct agacgtcctg cgggtaaagg gatgtcccac accaccacat aactcaggca
      841 tacgtccatc gcgtgtttgg cccagtccag gtatttatcc tctttgaacc gctcgtacag
      901 ttccaaaaaa ccctggaaag cagcccatgc cccctctttg tcctcgcagg tcgcatcgag
      961 ggtacctccc caatagcagc cgtccatggc aaggtgacga tccgcgtaga ttgcggcaat
     1021 tttttccgag gcttgtcggt attctttttt accaaacaga accgatgcta ttgccagtgg
     1081 tgccatgtaa aaaccttcgg cagtagagtg aggatcccag gccggattca atattcgttt
     1141 tacctgtcca tcgcatgccg atgtgagaaa tgcttcccat ttttccgtgt tgtaccgttt
     1201 gtttttttga gccgtttcaa ttgctttggc aaaattatac agcgcttgtc cgcacgaaac
     1261 atgatcaccc ccatagaatt cttttccgtt aaaacccaca ggaaacatcc cgttctccct
     1321 attcaccgga aatgatgcca gaaaatccaa cgattgctgt actttagccg gtaaatcctc
     1381 atcgtttaac cggtcggcaa gaacctgcaa cgcatatccc ggggattccg cctggccgca
     1441 ccatcccctc accatatctt ttctgttttg caaatcatac attccatagc ctgcggcatt
     1501 attgccgtaa tccacccacc ggctccgggc aaagcggtat ttgcttgcaa gtatcgtatt
     1561 gaaatccgga aacctctccg catcataggg tttgtgcaaa tccagtgaag tgtataccgg
     1621 ttgttgaaaa ccgctccctt cccgatcaat tgaatacagc tcgatataaa attctttttc
     1681 gataatacgt cccggctcca tactcaaata ggtattggta tatttcatgg gagttaactg
     1741 gagagccttg gctacactgt gttttttgtt gtagccgata gggccggaat aaaggacaaa
     1801 atctgtatac ccgtcaccag cctctacacc ggcagaccac cattgatcag ctagcaaagc
     1861 tccacgcacg gggctggggg taaaatgaat cgcggccgcg tattgatctt cccggttttc
     1921 cagcattaca aacggcatcg ggtaacgatg atcttcaaag atggcaaatt caccggcttt
     1981 ccctgtataa accggaataa tgtcgggatt gacacgcgct ccgtttttat ttccatagta
     2041 aagaatcccg ggcataaaaa cggacatctc cttccctttt acgcgcaaac gcacagagag
     2101 ggttaaagga gataatgtct tttccccttt ccattcaaac cgccgtacac atttgatcaa
     2161 tcccggcttt tcctcacgat aggcatccct catgtagaga actccatgcg gcaacacaat
     2221 ctctcctttc aggatagtcc attcatcgta ttgctccact tcagttggag tagcatggat
     2281 ccattctgtc atccagtcgt tttcccatcc tgtggcaacc gaccagactc cttcctccgg
     2341 agcggtaatg atcgcatcat tcttcaagac aaacaacggt ctgtgatctt ctactttcag
     2401 ctgcgcctgg ccttgcaggc ataataatag gcacgccagt accccgatcc ctgttttttt
     2461 cattttatcg caaacaaaat cattagttcg ttgagcggac agaaatacaa aataaattac
     2521 ttccttgtca cgacgacaaa attatcgatt ttttcctgtt ccctgaaaga ttctgtgtat
     2581 ctttgaatac aaaataataa tatggacctg aaaaatcaat cgttaacact ctttttcatt
     2641 attgttatca acgctttgcc gttatgcggt cagaccgtaa aaaagctatc ggaaattcct
     2701 atccgtgatc cttacattat gccagataaa aaaagcggat attactaca
//
LOCUS       DAOSDR010000403         2740 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrimonas sp. isolate AMR_MDS_1826
            NODE_13010_length_2740_cov_2.805959, whole genome shotgun sequence.
ACCESSION   DAOSDR010000403 DAOSDR010000000
VERSION     DAOSDR010000403.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863355
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrimonas sp. (wastewater metagenome)
  ORGANISM  Petrimonas sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Dysgonomonadaceae; Petrimonas.
REFERENCE   1  (bases 1 to 2740)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2740)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:10:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,720
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 35
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2740
                     /organism="Petrimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13010_length_2740_cov_2.805959"
                     /isolate="AMR_MDS_1826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2023866"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(146..1528)
                     /locus_tag="PLI30_12515"
     CDS             complement(146..1528)
                     /locus_tag="PLI30_12515"
                     /inference="COORDINATES: protein motif:HMM:NF018072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HOI80413.1"
                     /translation="MKKTAYPFSLKAIVAITVLMALLTSCLSDDLTVNEEPGVIVVSL
                     DGVTTRAAGDPLFTGDQAIDKVRVFVFAGEYVEKNKFYASGNSDFVNPFVLEVATGNK
                     EVYVVANESSALTPLLESITTKTGLMEILGDQISAPLTLPLLMTGKSVGVSVANIEDP
                     LRNNTSVTLTRVAAKISLTLKKDTDAEVKITRVSLLNNTEKTAIWAGGASISDQTYWN
                     WTHALPTAVDLSTTATAISGYENIYVYENLTGGVKAHATQLEVEALYNNVPTKYRVYL
                     NENVSSVINAGDPSSSVTTPGDHLYSLKRNHAYQLIGTVKNIGEFDGLTILSRVLPWN
                     YLPSSLLFDRTFTLFPKPTSNNFTYSVNENGKISFTFKLTNPIDATWVANLTNPTDFE
                     FEGIYQGGRNDEVTLTVKATNFPGAEERTTEFYINVLYGGNSSEIPLLSGSPLVGPGN
                     RVLIKQSANP"
     gene            complement(1539..2465)
                     /locus_tag="PLI30_12520"
     CDS             complement(1539..2465)
                     /locus_tag="PLI30_12520"
                     /inference="COORDINATES: protein motif:HMM:NF020418.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FimB/Mfa2 family fimbrial subunit"
                     /protein_id="HOI80414.1"
                     /translation="MNSTNKYSFILVAVLLTVMLSSCLKEDLSVCPRPFQITVKVVDA
                     DGNDITESGDVEQVIMFVFDEEEKIFKSFYLSASEVKQRKALQIVMDYPGHSLLKFVA
                     WGNLDEKVDYSNISDVKELKDLYVRLRSADSEQTDQRMAYSPSDLFYGTISVPVEYGG
                     TTSGTSHVLEITRKTAGVTITSLNLKQWNGNGEGSYSYSVRESLDTYDMNGNLTGTRS
                     FYSPPATFNKNGNFVAPIFYIFPAAFGKSIVVDILYNGEVIFTADRDSMGKPFNAEVG
                     RTLNILIDFEATLSINVNVTPWNQVFQYVEYL"
     gene            complement(2561..>2740)
                     /locus_tag="PLI30_12525"
     CDS             complement(2561..>2740)
                     /locus_tag="PLI30_12525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI80415.1"
                     /translation="YSYDQGYVADGTIKRNTQYNISLTIKGLGNPNIDPVDKAWLDVA
                     VAVEDWKVVGQGVIW"
BASE COUNT          654 a          638 c          633 g          815 t
ORIGIN      
        1 catcgacaaa ctaagtgtaa ggtggccgtc ttcggcggga aatttttctg tcacctcaga
       61 tgtgcaggcg gtcgccataa tcagcataac cgcaatcagt gaaacaaaag ggtatattct
      121 tttcttcata attaattctt catttttacg ggtttgcgga ttgtttgatt aataccctgt
      181 taccggggcc taccaaaggg ctaccgctga gcagcggtat ctccgatgag tttccaccgt
      241 acagcacatt aatatagaac tctgtcgtgc gttcttctgc acccggaaag tttgttgcct
      301 ttacggttag ggtcacctcg tcgtttctac ctccctggta tattccttcg aattcaaaat
      361 cagtgggatt ggtgagattc gctacccatg tggcgtcgat cgggttggtc agtttaaacg
      421 taaagctgat tttcccattc tcgtttacac tatacgtgaa attattggat gtcggtttgg
      481 ggaatagtgt gaacgtccta tcaaacaata aacttgacgg taaataattc cagggaagca
      541 cacgggagag aattgtcagc ccatcgaatt cgccaatatt tttaacagta ccgatgagtt
      601 ggtacgcatg gtttcttttc agggagtaga ggtggtcacc gggggtggtg accgacgagg
      661 aggggtcgcc cgcgttaatc acggaagaaa cgttctcgtt gagataaacc cggtattttg
      721 tgggtacgtt attgtacaag gcttccactt ccagttgtgt ggcatgtgcc ttaacaccgc
      781 ctgtaaggtt ctcgtatacg taaatgttct cgtatccgct gatggcagta gcagtagtgc
      841 tcaaatcgac ggcagtgggc aaggcatgcg tccaattcca gtaggtctga tcagagatac
      901 ttgcacctcc tgcccagatg gcagtttttt ctgtattgtt caacaggctc accctggtaa
      961 ttttcacttc ggcatccgta tcttttttca aggtcaggct tatttttgcc gctacccgtg
     1021 taagggtgac ggaagtattg tttcttaagg gatcctctat gttggcaacc gacacaccca
     1081 cagattttcc agtcatcaaa agtggaagtg tcagcggtgc gctgatctga tcgccaagta
     1141 tttccatcag tccggtttta gtggtgatgg attccagcag aggagtcagt gcactactct
     1201 cattggccac cacgtaaacc tctttgttgc ccgtggctac ttccaacaca aaaggattaa
     1261 caaaatcact gtttccggat gcataaaact tgtttttctc aacgtattca cctgcgaata
     1321 caaagacacg caccttatcg atagcttgat cgccggtaaa gaggggatcg ccagctgcac
     1381 gagttgtcac tccatcaagt gacacgacta taacacccgg ttcttcgttg accgtcagat
     1441 cgtcggagag gcatgatgtc aataaggcca taagtactgt tatagcgaca attgccttta
     1501 agctgaatgg atatgctgtt tttttcattg tctcgaattt atagatattc tacgtactgg
     1561 aagacctgat tccaaggagt gacattgaca ttgatgctta atgttgcctc gaaatcgata
     1621 aggatgttca gtgtccggcc tacctccgca ttgaacggtt ttcccatgct gtccctgtcg
     1681 gccgtgaaga tcacctcccc gttataaaga atgtccacca ctatcgattt tccaaatgcc
     1741 gcaggaaaaa tatagaaaat gggcgctacg aaattgccgt ttttgttaaa tgttgccggc
     1801 ggcgagtaaa aagaacgagt tcctgtcagg ttgccgttca tgtcataggt atcgagcgat
     1861 tcgcgcactg aataggagta ggagccttcc ccattgccgt tccattgctt caagtttagg
     1921 gaggtaatgg ttactcctgc ggtttttcgg gtgatttcca acacatgcga tgtgccggat
     1981 gttgttcctc catactctac cggtacgctg atggttccgt agaaaagatc actgggcgaa
     2041 tacgccatgc gttgatctgt ttgttcggaa tccgctgatc tcagcctcac gtagaggtct
     2101 ttcagttctt ttacgtcgct tatattcgaa tagtcgactt tttcatccag gttgccccaa
     2161 gcgacaaact tgagtaaaga gtggccggga taatccatca caatctgcaa tgccttacgc
     2221 tgtttcacct cgctggcgga gagatagaag ctcttgaata tcttttcctc ctcgtcgaac
     2281 acaaacataa ttacttgctc cacatctccc gattcagtga tgtcatttcc gtcggcatcc
     2341 actaccttaa cagttatctg gaacgggcgt ggacataccg acaagtcttc tttcaggcaa
     2401 ctgctcagca ttactgtcag caatactgct acaagtataa agctgtattt gttcgtgcta
     2461 ttcatctttt gttgttttat agtttcttct tgttcccgca acaggtatca ctcctgccgc
     2521 gggtatgttt tgcgggcaca gtgtggttac tgtggatagt ttaccagatt acgccttgtc
     2581 caacaacttt ccagtcctct actgccactg ctacgtccag ccatgctttg tcaaccggat
     2641 cgatattggg attacccaat cctttgatgg taagggaaat attgtattgg gtattgcgct
     2701 tgatcgttcc gtcagccaca taaccttggt cgtaagaata
//
LOCUS       DAOSDR010000404         2723 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrimonas sp. isolate AMR_MDS_1826
            NODE_13122_length_2723_cov_4.566717, whole genome shotgun sequence.
ACCESSION   DAOSDR010000404 DAOSDR010000000
VERSION     DAOSDR010000404.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863355
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrimonas sp. (wastewater metagenome)
  ORGANISM  Petrimonas sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Dysgonomonadaceae; Petrimonas.
REFERENCE   1  (bases 1 to 2723)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2723)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:10:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,720
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 35
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2723
                     /organism="Petrimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13122_length_2723_cov_4.566717"
                     /isolate="AMR_MDS_1826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2023866"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            166..393
                     /locus_tag="PLI30_12530"
     CDS             166..393
                     /locus_tag="PLI30_12530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI80416.1"
                     /translation="MRTTKKYKLKGRPTPRSKKAEFMLSDEEYDVINFYLKKYKITNR
                     SRWFRETILNHILKNMDMDYPTLFEENEMRR"
     gene            397..831
                     /locus_tag="PLI30_12535"
     CDS             397..831
                     /locus_tag="PLI30_12535"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005925954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0052717 - tRNA-specific adenosine-34
                     deaminase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HOI80417.1"
                     /translation="MLDDTYFMRQALIEAQRAYDKNEVPVGAVVVANHRIIARAHNLV
                     ETLNDVTAHAEMQAITAAANVLGGKYLTDCTLFVTVEPCPMCAGALGWAQLSRVVYGA
                     SDEKRGFSKSAPSVLHPKTTVTSGVLAEECAKLMKAFFEKIR"
     gene            851..2047
                     /locus_tag="PLI30_12540"
     CDS             851..2047
                     /locus_tag="PLI30_12540"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HOI80418.1"
                     /translation="MKTFYFLIISMLFFACGSHSNKSSDSIPNVKAIDDVMQAFVDSG
                     FIPGAVTAVVSKDNILHLGSVGVADYETRSPMQPDHIFWIASMTKPVTAVALLMLQDE
                     GKLSVDDPVSKYIPEFMDLKAPSGKDANLTITQIMNHTSGLREADAQTAMRAKELADL
                     IPSYVSGPTQFEPGSRWNYCQSGINTGARIVEVVSGLRFDQFLQQRIFDPLEMSSTTF
                     YPVWQSSTQRAAGYIKNRNNGRFEKAEVAEIFGKPDSAPLGNGGLFSTALDYAHFAQL
                     LLGKGIYKGKRYLSENAMTYLTSVKTGELDCGFLQEAQYGNLGANYGWGVGTCILRIP
                     HPGVAAMLSPGSFGHGGAWGTQAWIDPVRDIAYIMMIQRPNFNSDASNMRAEFQQKAF
                     DELAIK"
     gene            2242..>2723
                     /locus_tag="PLI30_12545"
     CDS             2242..>2723
                     /locus_tag="PLI30_12545"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019539491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HOI80419.1"
                     /translation="MNKKKIAVLIGSLRKESFNRKVANQLIQLAPDSLELEIVEIGQL
                     PHYNEDIDQNPPTEYVDFRRKIKAADGFLFVTPEYNRSVPSALKNAFDVASRPYGQSV
                     WGGKPGAVVSVSMSAIGGFGANHIVRQSMVFLNVPMMQQPEAYIGSAHTLFDENGNLL
                     "
BASE COUNT          802 a          580 c          602 g          739 t
ORIGIN      
        1 ttacggcctc ctcttcgcct cttttgccta actcattgtg ttgtgccatt ttttgattaa
       61 aatacaaatt tatgcaatat tcttttcttt cgtgttaaaa tattttgttt ttttgtgcct
      121 gaaaaggatt tacgcaattt ttcagaataa tttcttcaac aagaaatgag aacgacaaaa
      181 aagtacaaat taaaaggacg tcccactcca aggtcgaaaa aagcagagtt tatgcttagc
      241 gacgaagaat acgatgttat caatttctat ttgaagaaat acaaaataac caatcgctcc
      301 cggtggttca gggaaaccat actgaatcat attctcaaaa atatggatat ggattatccc
      361 accctttttg aagaaaatga aatgaggaga taagatatgc tggacgacac ttactttatg
      421 cggcaggcgc tgattgaggc gcagagagct tacgataaga acgaggttcc ggtaggtgca
      481 gttgtagttg ccaaccaccg gattatcgcc cgggcacaca acctggtgga gaccctcaac
      541 gatgttaccg cacacgccga aatgcaggcg ataaccgcag ccgccaacgt gcttggtgga
      601 aaatacctaa ccgactgcac gctttttgtc actgttgaac cttgtcccat gtgtgccggc
      661 gcgttgggct gggcacaact ctcacgggtt gtttacggcg caagcgacga aaaacggggg
      721 ttttccaagt cagcgcctag cgttttacat cctaagacaa ccgttacctc aggggttttg
      781 gccgaagaat gtgcaaagct gatgaaagct ttttttgaaa aaataagata accacaccat
      841 acacattgtc atgaaaacat tctatttttt aattatcagc atgttgtttt ttgcctgcgg
      901 ttcacattcc aacaaaagca gcgacagcat cccaaatgtc aaagccatcg atgacgttat
      961 gcaggcattt gtagattcgg gttttatacc cggagcagtt accgcggtgg tttcgaagga
     1021 taacatcctg catctcggga gtgttggtgt tgcagattat gagacaaggt cgcccatgca
     1081 acccgaccac attttctgga ttgcctccat gacaaaaccc gtcacagctg ttgccttact
     1141 gatgcttcag gatgaaggaa aactttctgt cgacgatccg gtttcgaaat acattcccga
     1201 attcatggac ttaaaagcac cctctggaaa ggatgccaat ctcaccatca cacagataat
     1261 gaaccacact tcgggtttac gggaagcgga tgctcaaacg gccatgaggg caaaagagct
     1321 tgcagacctg atcccttctt atgtcagcgg ccctactcaa tttgaaccgg gcagcagatg
     1381 gaactattgc caatcgggga tcaataccgg agcaagaatt gtggaagtgg tcagtggact
     1441 acgttttgac caatttctcc agcaacgtat ttttgatccg cttgaaatga gtagtaccac
     1501 tttctatcct gtctggcaga gcagtactca acgtgctgca ggctatatta aaaacaggaa
     1561 caacggacgg tttgaaaaag ctgaagtagc tgagatcttt ggcaaacccg atagtgcacc
     1621 actgggtaat ggtggactgt tttccactgc tctcgactat gcgcacttcg cacagttgct
     1681 tttgggaaag ggtatttaca aggggaaacg ctacctcagc gaaaatgcca tgacttacct
     1741 cacttcagta aaaacgggtg aacttgactg tggttttctt caggaggctc aatacggaaa
     1801 ccttggagca aattacggat ggggagttgg cacctgcatt cttagaattc ctcatccggg
     1861 agtagcagcc atgttatcgc ccggctcatt cggacacggc ggcgcctggg ggacacaagc
     1921 atggatagat cccgttaggg atatagccta tataatgatg atacaacgtc cgaatttcaa
     1981 ttcagacgca agtaacatgc gtgccgaatt tcagcaaaag gcatttgatg aattggcaat
     2041 taaatagata tagcctatta aacctgcatt aatatttttt aaatctatgc ttttccggtt
     2101 agatttagtt aaattatttt ctaatcccag actggtaaaa actttttagg tcctatcctt
     2161 gttaaagtcg gagaatgtgt aaacaaaaga catccaggct ctttataaaa ataacacttt
     2221 aaaaatcaaa ttttatttat tatgaacaag aagaagatcg ctgttttaat aggcagctta
     2281 agaaaagaat cgtttaacag aaaagtagct aatcagctta tacagctggc tcccgattcc
     2341 ttggaattag aaattgtaga aatagggcaa ttacctcatt acaatgagga tatagatcaa
     2401 aatccgccaa cagaatatgt cgattttcgc cgtaaaataa aagctgccga cggcttttta
     2461 tttgtaacac ccgaatataa ccgatcggtt ccaagcgcgc tgaaaaatgc tttcgacgta
     2521 gcctccagac cttatggaca aagcgtttgg ggagggaaac cgggagcggt tgttagcgta
     2581 tcaatgagtg ctatcggtgg atttggcgct aatcatattg tacgtcaatc gatggttttt
     2641 ctgaatgttc ctatgatgca gcaacccgag gcgtatatcg gaagcgccca cactcttttt
     2701 gatgaaaacg gaaatcttct gaa
//
LOCUS       DAOSDR010000405         2717 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrimonas sp. isolate AMR_MDS_1826
            NODE_13150_length_2717_cov_4.310293, whole genome shotgun sequence.
ACCESSION   DAOSDR010000405 DAOSDR010000000
VERSION     DAOSDR010000405.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863355
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrimonas sp. (wastewater metagenome)
  ORGANISM  Petrimonas sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Dysgonomonadaceae; Petrimonas.
REFERENCE   1  (bases 1 to 2717)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2717)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:10:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,720
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 35
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2717
                     /organism="Petrimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13150_length_2717_cov_4.310293"
                     /isolate="AMR_MDS_1826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2023866"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1096)
                     /locus_tag="PLI30_12550"
     CDS             complement(<1..1096)
                     /locus_tag="PLI30_12550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI80420.1"
                     /translation="MHLFNPENDLALANFGINYTPPASAVKIADDLAILPMWYAPEGA
                     KVIAATAQNEQFLSRLKEFFALEVSLIPFSGISLFPKEKIIPWGWNPALRKKLLDAGA
                     MEESLPSMEELKQLRSDSGRKNAIHILNELRAENNAFCGESYFFSAVDDVLHFLHSRN
                     GDHVLKMPNSGSGKGLSWIKGKITDKQTDWCRRVIHEQGGLIAEPVLNKIRDFALEFC
                     LSDNKAEFIGYSLFQSTSSGAYMGNFLMPQTDIEERLGEYTGPELLPQLRKSLAEKLC
                     RQYPSYSGCLGVDMMVCKTNRGYQIQPCVEINLRMNMGIVAYILYDRFVKPGFTGKFT
                     VDFFKKTGGALTFQQKMSTDFPLIVENGKII"
     gene            complement(1106..2185)
                     /gene="buk"
                     /locus_tag="PLI30_12555"
     CDS             complement(1106..2185)
                     /gene="buk"
                     /locus_tag="PLI30_12555"
                     /EC_number="2.7.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019540106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047761 - butyrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="butyrate kinase"
                     /protein_id="HOI80421.1"
                     /translation="MDNHPRILVINPGSTSTKIAVYKNGDVVFLKTIRHSAEELSQFK
                     RISDQYEFRKNIIYKELKNAEIRLDLIRIVVGRGGIVKPIASGVYRVNETMKRDLIEA
                     KRGEHASNLGALIADDIAKQLPSAEAFIADPVVVDELEPIARYSGHPLFERVSLFHAL
                     NQKAIARAHAKSLMRKYEDMDLIVVHLGGGISVGVHHKGRVVDVNQALDGDGPISPER
                     SGTLPVGELLKMAFSGKYTYEEMKSMVVGKGGLYAYTGLNNAYEVEKAAQAGDKKCYD
                     VMEAMAYQTAKEIGAFSTVLKGDVDAILITGGMANSKWFCNLIIERIHKIAPVYVYPG
                     EDEMKALAENGALILSGEIEPKEYV"
     gene            complement(2197..>2717)
                     /locus_tag="PLI30_12560"
     CDS             complement(2197..>2717)
                     /locus_tag="PLI30_12560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019540105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phosphate acyltransferase"
                     /protein_id="HOI80422.1"
                     /translation="FFESPYYHKLLCVTDAAMNIAPDFDTKVHILNNAVEACHKLGIT
                     EPKVAVVAAVEIVNPKMEATVHAALLKEMNIKKQLQGCIVDGPFAIDIAVDKEAAAHK
                     GIVSEVAGDSDIILVPDIEAGNIFYKALNFLGGAVCAAVITGAAVPIVLTSRSDSETS
                     KMLSIALAAAIH"
BASE COUNT          642 a          655 c          646 g          774 t
ORIGIN      
        1 aaataatttt cccgttttca acgatgaggg gaaaatctgt tgacattttc tgttggaagg
       61 ttaacgctcc gcccgttttt ttgaaaaaat cgaccgtaaa ttttccggta aacccgggtt
      121 ttacaaaacg gtcgtagaga atgtaggcga caatccccat attcattcga aggttaatct
      181 caacgcaagg ttggatttga taccctctgt tggttttgca aaccatcatg tccactccca
      241 gacaaccgga ataggatggg tactggcggc agagtttttc ggcaagagat ttccggagtt
      301 gcggcagcaa ctccggaccg gtgtattcac ctaacctttc ttcaatatcg gtttgtggca
      361 tcaggaagtt gcccatgtag gcacctgaag atgtggattg gaacagggag tacccgataa
      421 attcagcttt gttatcggaa aggcagaatt ccaaggcaaa atctcgaatc ttgttcagaa
      481 caggttccgc aataaggcct ccctgttcgt gaattacccg ccggcaccaa tcggtttgtt
      541 tgtcggttat tttccctttg atccagctta atcccttgcc gctgccggaa ttgggcattt
      601 tcaatacgtg atccccgttt cgcgaatgta aaaagtgcaa tacatcatca acagcggaga
      661 aaaaatagga ttctccacaa aacgcgttat tctccgccct gagttcgttc agaatatgta
      721 tggcgttctt cctgcccgag tcgcttcgaa gctgttttaa ctcctccatc gaaggcaggc
      781 tctcttccat tgcaccggcg tccagcaact tttttctcaa tgcggggttc catccccacg
      841 ggataatttt ctctttcgga aaaagggaaa tgcccgaaaa agggatgagg gaaacctcga
      901 gagcgaaaaa ctctttcaat cgggaaagga actgttcgtt ttgagcagtg gcggcaatca
      961 ccttagcacc ttccggagca taccacatcg gcaaaatggc cagatcgtcc gcaattttca
     1021 ctgcagatgc cggaggcgtg tagttaatac cgaagttcgc cagggccaga tcgttctcgg
     1081 gattgaagag atgcatacgc ttgggttaca cgtactcttt cggttcaatc tctccgctta
     1141 ggatcagtgc accgttttca gccagggctt tcatttcatc ctcacccgga taaacgtata
     1201 ccggtgcgat tttatggata cgttcgatga tcaggttgca gaaccatttg ctgttagcca
     1261 ttcctccggt gatcaggatg gcatccacat cgcctttcag tacggttgag aatgcgccga
     1321 tttcttttgc cgtctgataa gccatggctt ccatcacatc gtagcatttt ttatctccgg
     1381 cctgagcggc tttttccact tcgtaggcat tgttgaggcc cgtataggca tacagtcctc
     1441 ctttgccgac caccatcgat ttcatttctt cgtaagtgta cttgccgctg aaagccatct
     1501 tcagaagttc gcctacgggg agtgttccgg accgttccgg tgagatgggc ccgtcaccgt
     1561 caagcgcctg gttaacgtca acgacccgtc ctttgtgatg tacgcctacc gatattcctc
     1621 cccctaagtg cactacaata aggtccatgt cttcatattt tctcatgaga gacttggcgt
     1681 gagcgcgggc aatggctttt tggttgagcg catggaaaag agagactctt tcgaaaaggg
     1741 gatgtccgga ataccgggca atgggttcaa gttcgtccac taccaccggg tcagcaataa
     1801 aggcttctgc tgaagggagt tgtttggcaa tgtcatcggc aataagtgct cctaggttgc
     1861 tggcgtgttc tcctcttttt gcctcgataa ggtctctctt cattgtttcg tttaccctgt
     1921 aaactcccga cgcaataggt ttcactatgc ctccacgtcc gacgacgatc cggataaggt
     1981 ccaacctgat ctcggcgttt tttaattctt tgtaaataat atttttacga aattcatact
     2041 gatcgcttat tcttttaaat tggcttaatt cttcagccga atgacggatt gtttttaaaa
     2101 aaaccacatc tccgttttta tagacggcaa tttttgttga tgttgaaccc gggttaatca
     2161 ccaggatccg cggatgattg tccataaggg tagtgtttag tgtatagctg ctgccaaagc
     2221 gatggaaagc atcttactgg tttctgaatc agaacgcgaa gtgagtacaa taggaaccgc
     2281 tgcgcccgta atgacggctg cacagacggc ccctcccaag aagttcaatg ccttgtagaa
     2341 aatatttccg gcttcgatgt cgggcacgag aatgatgtcg ctatctccgg caacctcact
     2401 gacgatgcct ttgtgtgcgg cagcctcctt gtcgacagcg atatcgatgg cgaagggccc
     2461 gtccacgatg catccctgca attgtttttt gatgttcatc tcttttagca gagcagcatg
     2521 aacggtggct tccattttag ggttaacaat ttccacggct gcgacaacag ctactttcgg
     2581 ttcagttatc ccgagtttgt gacacgcctc tacggcattg ttgagaatgt gtactttggt
     2641 gtcgaaatcg ggagcgatgt tcatggctgc atccgttaca cataagagtt tgtggtagta
     2701 gggagactcg aaaaaag
//
LOCUS       DAOSDR010000406         2705 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrimonas sp. isolate AMR_MDS_1826
            NODE_13211_length_2705_cov_3.459245, whole genome shotgun sequence.
ACCESSION   DAOSDR010000406 DAOSDR010000000
VERSION     DAOSDR010000406.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863355
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrimonas sp. (wastewater metagenome)
  ORGANISM  Petrimonas sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Dysgonomonadaceae; Petrimonas.
REFERENCE   1  (bases 1 to 2705)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2705)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:10:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,720
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 35
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2705
                     /organism="Petrimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13211_length_2705_cov_3.459245"
                     /isolate="AMR_MDS_1826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2023866"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..214
                     /locus_tag="PLI30_12565"
     CDS             <1..214
                     /locus_tag="PLI30_12565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI80423.1"
                     /translation="NNNGTVKVDYNVRRTDGGGLGGNETRVAFNVYAYNRYTSQGMNQ
                     TLWLNRVHEADESSSGASGWLVHLEP"
     gene            455..706
                     /locus_tag="PLI30_12570"
     CDS             455..706
                     /locus_tag="PLI30_12570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI80424.1"
                     /translation="MKGSPVVRIAARGGTREDSNPTNVTLSRPVTAGMQVCEPDPVRD
                     TVTFEAQSHGQSATSVRVTRRIGRYMPAKGVVSVQAVGR"
     gene            1044..>2705
                     /locus_tag="PLI30_12575"
     CDS             1044..>2705
                     /locus_tag="PLI30_12575"
                     /inference="COORDINATES: protein motif:HMM:NF024753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HOI80425.1"
                     /translation="MEHISIWKRMIISAILLVLFLAVPALAGTVIPDDKSSANPDFIA
                     AANDTGVGTLEAGIVATGSESGDSGSGNGGLADTPWPKFGGDLKNTGVSPYVGTQATT
                     PKWQYDPEGGTGFGYSTPVIGSDGTIYIGTRDWRFYAFNPDGTTKWIYNDAGAYATGG
                     PIYGSAAIGSDGTIYFGTKSGETKLYALHPDGTLKWGSPTIGEISGSPAIGSDGTIYA
                     GSDQIYAFNPVDGTIKWSYPAGVTYACPAIGADGTIYVGSSDTNVYALNPDGTLKWNF
                     TTGGEIQGSPAIGADGTIYIGNYGDGNVYALNPDGTLKWNFAAGGSIQGSPAIGLDGT
                     IYIGTYGNNKVYALNPDGTEKWVYVLTNSVCGSPVIGADGTIYFVNKSRYLYALNPDG
                     TLKFQSARLPSGSSPEGTPVIGPDGTLYFGTSSTNSRFYAFPGVVTGTADTTSGTTPL
                     DVQFTGTSPLTVTAWHWDFGDGSTSTEQNPSHTYASAGTYTVNLTVTHADGTNYHRQV
                     EYIRVYAPPTAGFTANVTSGLFPLAVSFTDQSTCSPTSWAWDFEND"
BASE COUNT          702 a          679 c          721 g          603 t
ORIGIN      
        1 gaacaacaac ggtacggtga aggtcgatta caatgtccgg agaacagatg gaggaggact
       61 tggtggtaat gaaaccaggg ttgcattcaa tgtgtatgcg tataaccggt atacttcgca
      121 aggaatgaac cagaccctct ggctgaatcg ggtgcatgaa gccgatgaaa gttcctccgg
      181 tgcaagcggg tggttggttc atctagaacc ataaatgatg gtgataagga aactctcttt
      241 ccagtatttc cccctttttt tgaatctttt caaaccaagc atcatatcag agtatcgcgc
      301 ggattttgat gggtgggatg gaatgccatg aatcacacat gatgatggga atatgaccac
      361 acgcgaaatt gcggagagcg acgtaggagt tgtgaatgta agatgacaaa gggtattaat
      421 gcaccatttc accttacttt gaaggctttg gtgtgtgaag ggatcgccgg tcgttcgcat
      481 cgccgcaagg ggaggcacac gagaagatag caaccctacc aacgtcacgc tttcgcgtcc
      541 ggtaacggcc gggatgcagg tatgtgagcc cgatccggta cgggacaccg taacattcga
      601 ggctcaatct cacggacaat cagcgacgtc ggtcagggtt acccggcgga tcggccggta
      661 catgcctgca aaaggggtcg tttcggtgca agcggtcgga cggtgacggc gcccggaggc
      721 gagtgaacga tcgggtgaac cggccccttc cgacgcacaa ccgtttcggg aattcaactg
      781 cctctcctca catcgaacat acaacgatca gtggttttga aggtggtcag atctcattat
      841 cgtaaagaag tactattttt aagttctgct tttcagacat ccatctttaa ggctcggttt
      901 tggaaataaa atgtctttaa ttaactcttc tcgaaatgaa aaggaataaa ttgtctgata
      961 tctcaatata aaataagttg gcaaactgtt tttgttaact taatgacatt aactgggtcg
     1021 tgtcgatact gactggtgat cctatggaac atatctcaat ctggaaacga atgattatat
     1081 cagcaatact gctggtactg tttctggcgg tgcctgccct ggcgggaacc gtcatccctg
     1141 acgacaaaag cagcgcaaac cccgacttca tcgcggctgc aaacgatacc ggtgtaggca
     1201 cccttgaagc gggcatcgtc gcgaccggca gcgaaagcgg cgattccggc agcgggaacg
     1261 gcggcctcgc cgatacgccg tggccgaagt tcgggggaga tctcaaaaac accggtgtat
     1321 ccccctatgt cggtacacag gcaactacac ccaaatggca atatgaccct gagggtggaa
     1381 ctggattcgg ctactcaact ccggttatcg gatcggatgg aaccatctac attgggacaa
     1441 gggattggag attctatgct ttcaatccgg acgggaccac aaaatggatc tacaacgatg
     1501 ccggggctta cgccacaggc gggcctattt acggatcagc ggcaatcgga tcagatggaa
     1561 ccatctactt tgggacaaag agcggtgaga caaaactgta tgcattacac cccgacggta
     1621 ccttgaagtg gggatctccc accattggag agatatcggg cagcccagca atcggttcgg
     1681 atgggacaat ctacgctggc agcgatcaaa tatacgcgtt caatccggtt gacggaacta
     1741 tcaaatggag ctacccagca ggcgtaactt atgcctgccc tgcaatcggt gcagatggga
     1801 cgatttatgt cggaagctcg gatacgaacg tttatgctct aaacccggac ggaaccctga
     1861 aatggaactt tactacagga ggagaaattc aaggctctcc ggcgattggg gcggatggaa
     1921 ccatctatat cggaaattat ggcgacggca atgtctatgc gttaaaccca gacggaaccc
     1981 tgaaatggaa ttttgctgca ggaggatcca ttcaaggttc gccggcaatt ggattggatg
     2041 gaaccatcta tattggaacg tatggtaata acaaagtcta tgcgttaaac ccggatggaa
     2101 ccgaaaaatg ggtttatgtg cttaccaata gcgtctgtgg ttccccggta attggagcgg
     2161 acggaaccat ctatttcgta aataagagca gatatttata cgcactgaat ccggacggaa
     2221 ctctgaagtt ccagtctgcg cgtcttccat cgggaagcag cccggagggt actcctgtaa
     2281 tcggtcccga tggaacgctg tattttggaa catctagtac aaactccaga ttttacgcat
     2341 tcccgggcgt cgtcaccggc accgcagaca ccaccagcgg caccaccccg cttgacgtcc
     2401 agttcaccgg cacctcgcca ctcaccgtga ccgcatggca ctgggacttc ggagacggca
     2461 gcacctccac ggagcagaac ccctcgcata cctacgcatc ggctggcacc tacacggtca
     2521 acctgaccgt cacgcacgcc gacggcacga actatcatcg ccaggtggag tacatcaggg
     2581 tgtatgcacc cccgaccgcc gggtttacgg caaatgtcac ttcaggacta ttcccccttg
     2641 cggtcagttt cacagatcag tcgacctgct ccccgacctc ctgggcctgg gacttcgaga
     2701 acgac
//
LOCUS       DAOSDR010000407         2689 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrimonas sp. isolate AMR_MDS_1826
            NODE_13298_length_2689_cov_3.139711, whole genome shotgun sequence.
ACCESSION   DAOSDR010000407 DAOSDR010000000
VERSION     DAOSDR010000407.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863355
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrimonas sp. (wastewater metagenome)
  ORGANISM  Petrimonas sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Dysgonomonadaceae; Petrimonas.
REFERENCE   1  (bases 1 to 2689)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2689)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:10:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,720
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 35
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2689
                     /organism="Petrimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13298_length_2689_cov_3.139711"
                     /isolate="AMR_MDS_1826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2023866"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..529)
                     /locus_tag="PLI30_12580"
     CDS             complement(<1..529)
                     /locus_tag="PLI30_12580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019540042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOI80426.1"
                     /translation="MEKNFPDYVRNEDHALFLLIKKRDKDAFAAVYQKYHRYLYSLAV
                     KYLKDAQLAEDAVQHVFVKLWETTKNIEIEINLKNYLYTMTKNYILNTIRDHKEEISL
                     SYANAQIDIPGQDDIIKALENKQMRSILYQGVESLPPQKKEVCLRKLETTDSNQQIAD
                     KMGISVHTVKSHYQES"
     gene            complement(552..1766)
                     /locus_tag="PLI30_12585"
     CDS             complement(552..1766)
                     /locus_tag="PLI30_12585"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009237051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HOI80427.1"
                     /translation="MINVNDIRADFPILSLQVHGKPLVYLDNGATTQKPLCVIDKIRE
                     MYCSVNANVHRGVHFLSQAATDEHEASRRTVQEFIGAASSNEIVFTRGTTEAINLISS
                     SFCREFCKPGDEVIISAMEHHSNIVPWQLQEAISGIKLRILPISDEGEILLEELEKLI
                     SSRTKLIAVTHVSNVLGTITPIEKIIETAHRYDIPVLVDAAQSVQHIPVDVQRMDCDF
                     LVFSSHKIYGPTGMGVLYGKEKWLDKLPPYQGGGEMIATVSFEKTTFAGLPFKFEAGT
                     PDYVGSTALATALKYISRIGIENIAKHEQGVLAYATEQLLSIDKLKIYGTSKNKCSVI
                     SFLVDGVHHYDMGVLLDTMGIAVRTGHHCAEPLMRRLGVEGTVRASFAVYNTREEVDV
                     LVNGIRRVVKMF"
     gene            complement(1763..>2689)
                     /gene="sufD"
                     /locus_tag="PLI30_12590"
     CDS             complement(1763..>2689)
                     /gene="sufD"
                     /locus_tag="PLI30_12590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006799335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HOI80428.1"
                     /translation="SDNGVVAYNTMFAQDGFVIYVPENVVIEKPLQLINILRGGVDLN
                     VNRRILIVAEKNAQVKLLVCDHTVDDVQFVVTQVAEIIADRDAQVDFYELEENSVKVK
                     LLNNVFAEQAENSNVTVSTITLHNGFTRNNYRFRLLGEYAEAHIGGLAICDKEQHVDN
                     YAFLEHATPNCHSNELFKYALQERSTGVFCGRILVDKGAQKTLAYQNNKNLVTSDEAK
                     MFSKPQLEIYADDVKCSHGLTTGQLDQDALFYLQARGIPEEEARMLLMVAFTQDVVDL
                     VRIDSLQDRLVQLINKRFRGELMRCGNCHVCK"
BASE COUNT          672 a          675 c          580 g          762 t
ORIGIN      
        1 ctgactcctg atagtgtgat ttaactgtat gaactgaaat ccccatcttg tccgcaattt
       61 gctgattact atccgttgtt tcgagctttc tcaggcaaac ctcttttttt tggggaggaa
      121 gcgattcaac tccctggtag agaatactcc tcatctgttt attttccaat gccttaatta
      181 tatcgtcctg tcccggaata tctatctgcg cattggcata actgagggat atctcttctt
      241 tatgatcgcg tatcgtattt aatatgtaat tttttgtcat cgtatagagg taattcttaa
      301 ggtttatctc tatctcgata tttttggttg tttcccataa tttcacgaaa acgtgctgaa
      361 cggcatcttc tgccaattgt gcatctttca agtacttgac agccaatgaa tacagatacc
      421 ggtggtattt ctgatacacc gccgcaaaag cgtctttgtc tctcttttta atcaagagaa
      481 acaacgcatg gtcttcgttt ctaacgtaat cgggaaagtt tttttccata tcgccacatt
      541 ctcccaaacg gtcaaaacat cttaactacc cgtcggattc cgttcaccag gacatccacc
      601 tcttccctgg tgttgtacac cgcaaacgat gcccggacag ttccctctac acccaaccgg
      661 cgcatcagcg gctcagcgca atggtggcca gtacggacag caatccccat cgtgtcaagc
      721 aatacaccca tatcgtaatg atgaactccg tccacaagga aagagattac actgcatttg
      781 ttttttgagg ttccgtatat tttgagttta tcgatagaca acaactgctc tgtggcatac
      841 gcaagcactc cctgttcatg cttcgcaata ttttctatgc cgatacgtga tatatatttc
      901 agtgctgtgg ccagcgctgt ggaccccaca tagtcggggg tgcctgcttc gaacttgaac
      961 ggcaacccgg cgaaagtggt tttttcgaac gatactgtcg caatcatctc accgccgccc
     1021 tggtagggag gaagtttatc gagccacttt tctttcccgt acagcactcc cataccggtt
     1081 ggtccataga ttttgtgtga cgaaaacacg agaaaatcgc aatccatccg ttgaacatca
     1141 accggtatgt gttgaaccga ttgcgcggca tcaaccaaaa cagggatatc gtaccggtgt
     1201 gccgtttcaa taattttctc tatcggagtg atcgtaccga gaacgttgga gacatgagtg
     1261 acggcaatca gctttgtgcg ggaagagatt aatttttcca gctcttccaa aagaatttct
     1321 ccctcatccg agataggcaa tatccgcagt ttaattccgg agattgcctc ctgcagctgc
     1381 caaggaacaa tgttggaatg atgttccata gcggaaatta tgacctcgtc accgggtttg
     1441 cagaactcac ggcagaacga agaggagatc aggttaatgg cttccgtggt accgcgtgta
     1501 aagacaatct cgtttgaaga agcggcaccg ataaactcct gcaccgttct tctcgatgct
     1561 tcgtgctcgt cggtggcggc ctggctcaaa aaatgcacac cgcggtgcac gttggcattc
     1621 acggaacaat acatctcccg gattttatct atcacacaaa gcggtttttg tgttgtcgca
     1681 ccattgtcca ggtaaacaag cggtttaccg tgtacctgaa gcgatagaat gggaaagtca
     1741 gcgcgtatgt cgtttacatt aatcatttac acacgtggca atttccgcaa cgcatcagtt
     1801 cgcccctgaa gcgcttgttg atcagttgta caagcctgtc ctgaagcgaa tcgatacgga
     1861 ccaggtctac tacatcttgc gtaaatgcca ccatcaggag catgcgggct tcttcttcag
     1921 gaattccgcg cgcctgcaga taaaacaggg catcctgatc gagctgtccg gtagttaacc
     1981 cgtgcgagca ttttacatca tcggcataaa tctcaagctg cggcttcgag aacatcttgg
     2041 cttcatccga ggtgacaagg ttcttgttgt tctgataggc cagtgttttt tgcgcaccct
     2101 tatccaccag tatccttcca cagaaaaccc ccgttgatct ttcctgaagt gcatatttaa
     2161 acaactcgtt actgtggcag ttgggagtag cgtgctctaa aaatgcataa ttatcaacat
     2221 gctgctcctt gtcgcaaatg gccagccccc cgatatgggc ctcagcgtat tcacccagca
     2281 ggcggaagcg atagttattc cgggtgaacc cattatgcag tgtgatcgta gagaccgtta
     2341 cgtttgaatt ttcggcctgc tcggcgaaaa cattgttcaa taatttgacc tttaccgaat
     2401 tctcttccag ttcgtaaaaa tccacttgag catctctgtc ggcgataatc tccgctacct
     2461 gtgtcacgac aaactgaaca tcatcaaccg tatggtcgca aacaagcagt ttaacctgtg
     2521 catttttttc agctacaata agaatgcggc ggttcacgtt caggtcaacc cctccccgga
     2581 ggatattgat caattgaagc ggtttttcga tgaccacatt ttcggggaca taaatcacaa
     2641 aaccgtcctg ggcaaacatg gtgttatatg ccactacacc gttatcgct
//
LOCUS       DAOSDR010000408         2669 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrimonas sp. isolate AMR_MDS_1826
            NODE_13424_length_2669_cov_4.273527, whole genome shotgun sequence.
ACCESSION   DAOSDR010000408 DAOSDR010000000
VERSION     DAOSDR010000408.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863355
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrimonas sp. (wastewater metagenome)
  ORGANISM  Petrimonas sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Dysgonomonadaceae; Petrimonas.
REFERENCE   1  (bases 1 to 2669)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2669)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:10:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,720
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 35
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2669
                     /organism="Petrimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13424_length_2669_cov_4.273527"
                     /isolate="AMR_MDS_1826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2023866"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..812
                     /locus_tag="PLI30_12595"
     CDS             <1..812
                     /locus_tag="PLI30_12595"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HOI80429.1"
                     /translation="DFPIAGTYHTDIPQYVRTLTSDEMLEKAAWQFMIWFYGRMDEVM
                     VPSASTRRQLVERGLAEEKMKPLPRWVDVRRFSPEMRNPGFWDGYSANRSMKFIYVGR
                     VSREKNLELLADAFMEVAAGALPAQLVIVGDGPYLGELRKKLSGYPVIFTGFLQGAEL
                     SVAYASSDVFVFPSTTDTFGNVVLEAQASGLPVIVSDSGGPKELMLDGVTGFVAKADN
                     RKEFADSMRSMLRDPAMTTKMGKNARSFTEQGAAADTDACYAILRPTATTF"
     gene            complement(854..991)
                     /locus_tag="PLI30_12600"
     CDS             complement(854..991)
                     /locus_tag="PLI30_12600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI80430.1"
                     /translation="MPLDSYSRWEGKAEMLDGTRVFGEPGEYRHPTARLEINPHYGSL
                     D"
     gene            1577..2287
                     /locus_tag="PLI30_12605"
     CDS             1577..2287
                     /locus_tag="PLI30_12605"
                     /inference="COORDINATES: protein motif:HMM:NF023568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HOI80431.1"
                     /translation="MGVVLLHGSGHPTKHIEGLINKLESNHFLVAHPEMPWSKDRQYD
                     APVDEAVAQVNSAFIKLKEQGAEKLFIAGHSKGGVFALYYASKYPVYGLISIAPGGNV
                     GGKNFRNKLQSSVEKAKELIASDKGNKKATFSDLEGARGITEVEAPAAAYLTWYDPDG
                     AMNSKNSATRISANVPILWIVAKGDYEGLRQTNIPMFDLFPSNPKTRLYEPSTSHLNA
                     PLDAADEIIKWISEVSNP"
     gene            2387..2662
                     /locus_tag="PLI30_12610"
     CDS             2387..2662
                     /locus_tag="PLI30_12610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI80432.1"
                     /translation="MHFFAKYLWKAVMDVRDMQGNPAIWERLWWSDLSSKEKELWTLL
                     GWHQDLWDRNEAPPSADKAWNDLDSLEQYAAKSLGFTEDMWNGFEDE"
BASE COUNT          662 a          652 c          716 g          639 t
ORIGIN      
        1 tggatttccc catcgccggc acctaccaca ccgacatccc ccagtacgtg cgcaccctca
       61 cttccgacga aatgctggaa aaggcggcct ggcaattcat gatctggttc tacggccgga
      121 tggatgaagt catggtccct tcggccagca ctcgtcggca acttgtcgaa cgcggcctgg
      181 ccgaagagaa gatgaagccc ctgccccgct gggtggatgt gcggcgcttt tcgccggaaa
      241 tgcgcaatcc cgggttctgg gacggatatt ccgccaacag atcgatgaaa ttcatctatg
      301 tgggacgggt ttccagggaa aagaacctgg aacttctggc ggacgcgttc atggaagtgg
      361 ccgccggcgc gttgccagcg cagctcgtca tcgtgggtga cggtccctac ctgggtgaac
      421 tcaggaaaaa actcagcggg tatccggtta ttttcaccgg tttcctccag ggggcagagc
      481 tgtcggtcgc ctacgcctcc tccgacgtgt tcgtcttccc gagcaccacc gacaccttcg
      541 gcaacgtggt gttggaggca caggcgtcgg gcctcccggt aatcgtttcc gacagcggtg
      601 gcccgaagga gttgatgctc gacggcgtta ccggtttcgt cgcaaaggct gacaaccgca
      661 aggagttcgc tgattcaatg cgttcgatgc tgcgggatcc cgcaatgacg accaagatgg
      721 ggaagaatgc ccgttctttc acggagcagg gagcggcggc cgacactgac gcctgctacg
      781 ccatcttgcg gccgacggcc actaccttct gagaatcgat gggttgaaag cgttccatcc
      841 ttggccggtt ctttcagtcc agtgagccgt aatgcgggtt tatttccagc ctagccgtcg
      901 gatggcgata ctctcccggc tcgccgaata cccgtgttcc atccagcatc tccgccttcc
      961 cttcccacct tgaatacgaa tcgagcggca caaaacgacc ctgacttttc catgaatttt
     1021 ttgttttttc cctcttttcc cgtcactgcg ctacccttac cggcgcctcc tcaacattgt
     1081 aaatattgac ttatctcttg aagtaacggt tataatttcg tactcatagt gtttaatgaa
     1141 aggtactccc gaggggatgc ggcattgcgc tggagtcctg gaaaaaacgg aaggccactt
     1201 tcgtcagtcg ggtgctgggg gcggcctatt ttcgctaaac gggatttttt tatgagacct
     1261 taggagttac tccgacaagc gttcagattg aacggcgcat tgccggggca aggctggaaa
     1321 agtttatagt attggaaacc aggaaaacga agcaaaatcg gatatattta tactattaga
     1381 aacccgtcac gcttgtagta ccacaaacgt gtttataaca atataaaccg gtttatacat
     1441 ataaaaatcc ggcctggccg gtcaataagt agaaaggagg atgtacgtga aaacattcat
     1501 caaccaacta gtctttgcaa tcgttgtgtt ctctttatcg gtactgccgt ctctagcaat
     1561 tagcggcgag gcaaaggtgg gggtcgttct tctgcacggt agcggacatc cgacgaaaca
     1621 tattgaaggt ttaataaaca aactggaatc gaaccatttt ctggttgctc acccggaaat
     1681 gccgtggtct aaggatcgac agtacgatgc gcctgttgat gaagctgtgg cgcaggtaaa
     1741 ttcggctttt atcaagctaa aagaacaggg ggcggagaag ctttttattg cgggccacag
     1801 caaaggtggc gttttcgctc tttactatgc aagcaaatat ccggtctacg gactcatctc
     1861 cattgctccc gggggtaacg ttgggggcaa gaatttcagg aataagctcc agagttcggt
     1921 tgagaaagcc aaggaactca ttgcatcgga taagggcaac aaaaaggcga cgtttagtga
     1981 tctggaaggc gccagaggta tcacggaagt tgaggctcca gcggcagcgt atctcacctg
     2041 gtatgatcct gatggtgcga tgaactctaa aaattccgca acacgtatca gtgcgaacgt
     2101 tcccattctg tggatcgttg caaaaggtga ttatgaggga ttgaggcaga ccaatatacc
     2161 gatgtttgat ttgttcccat caaatcccaa gacccgatta tacgaaccat ctaccagtca
     2221 tcttaatgca ccacttgacg cagcagatga aattattaaa tggatatcag aggttagcaa
     2281 tccctagcca aggcctgtag cggatttgct atggaaaggc gatggtcaac ccttgacatg
     2341 agacaatcct tcgtcattat caacggaaag agggacaagg gaacggatgc atttcttcgc
     2401 aaaatattta tggaaggctg ttatggatgt tcgggacatg caaggcaatc cggcaatttg
     2461 ggaaaggttg tggtggtcgg atctgagcag caaagaaaag gaactctgga cccttctcgg
     2521 ctggcatcag gatctgtggg ataggaacga agcgccgcca agcgccgata aagcctggaa
     2581 tgacttggat tctttagaac aatacgccgc aaagagtctt ggatttaccg aggatatgtg
     2641 gaacggtttt gaagatgagt aaatactac
//
LOCUS       DAOSDR010000409         2662 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrimonas sp. isolate AMR_MDS_1826
            NODE_13472_length_2662_cov_2.176064, whole genome shotgun sequence.
ACCESSION   DAOSDR010000409 DAOSDR010000000
VERSION     DAOSDR010000409.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863355
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrimonas sp. (wastewater metagenome)
  ORGANISM  Petrimonas sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Dysgonomonadaceae; Petrimonas.
REFERENCE   1  (bases 1 to 2662)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2662)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:10:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,720
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 35
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2662
                     /organism="Petrimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13472_length_2662_cov_2.176064"
                     /isolate="AMR_MDS_1826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2023866"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..587)
                     /locus_tag="PLI30_12615"
     CDS             complement(<1..587)
                     /locus_tag="PLI30_12615"
                     /inference="COORDINATES: protein motif:HMM:NF014732.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Wzz/FepE/Etk N-terminal domain-containing
                     protein"
                     /protein_id="HOI80433.1"
                     /translation="MTEQLQETNHSDEIDLKDLIVQLWKKRKFILAVTGLFFLLGIFI
                     AFTLPVEYTAQSVILPQSGRQSSLGNLGSLASIVGVNMGTTVMAEGNISTGIYPQIVN
                     SLPFVREIMETPIVVERSDGKEITLYEYYSEKKYRSKNVFTVIKKYTIGLPGTLISAF
                     RSSKNMQEVTIHTAITPDSTGIVSISRQEQAVYNA"
     gene            complement(604..>2662)
                     /locus_tag="PLI30_12620"
     CDS             complement(604..>2662)
                     /locus_tag="PLI30_12620"
                     /inference="COORDINATES: protein motif:HMM:NF014606.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SLBB domain-containing protein"
                     /protein_id="HOI80434.1"
                     /translation="DTIPPGKRIFGQDFFSRENLTFAPNVNMPTPANYVLGPGDEVII
                     DVWGDSELNVRYTITPDGYITVPGLGRIQLSGMNVDQATGRIRNEFSGIYSDLNSSQP
                     RTFLALSVGNIRTIKVNVMGEVTTPGTYTLTSFSSAFHALYAAGGITPIGSLRNIRVF
                     RGGRVAATVDLYEYLLKGNNMNDISLQDGDIVMVDPYVGLAQIAGEIKRPMKYEMRPD
                     ETLQDILGFAGGFTGEAYKNNIQVDRKGEYEKETFTVNQTGFASFTIQDGDFITVSTI
                     LNRFSNMVEITGAVYRPGQYAIGNQIRTVRDLVSIAQGITGDAYVYRALLYREQEDLK
                     QTMQSFDLDALLSNRIPDIPLQKNDRLHIPSIFSIEDSLTVYVGGEVRAPGNYPYAEN
                     MHLEDVVLRAGGLRESASTAQIDVYRRIKNPASTSSSSQTGEAFRFSLRDGIIISNDT
                     SFVLHPFDQVVIRRSPGYEIQQNITLRGEVLFDGQYAKIKKDERLSSFIQRAGGLTEY
                     AYAKGARLSRQLNEAERRRTRDALLARATAEGDSTYIENMDFSTQYVGIDLEKALKKP
                     GSDDDIILRQGDEIFIPQYEGTVKISGGVLYPNTVTYNKRMSLESYVRQAGGYSRLAM
                     KNKPFVIYMNGKVASGRWAKIEPGCEIIVPERPERESVGIQNILGMSTTLASLALIIS
                     RFF"
BASE COUNT          630 a          651 c          643 g          738 t
ORIGIN      
        1 atggcattgt acaccgcttg ttcctgtcgt gagattgata caataccggt gctgtcagga
       61 gtaatggctg tgtgtatggt cacttcctgc atatttttcg atgaacgaaa agccgatata
      121 agggtacccg gcaatccgat ggtgtatttt ttaataaccg taaaaacatt tttacttctg
      181 tatttttttt cagagtaata ttcgtacaga gttatctctt taccgtcaga tctttcaaca
      241 acaatcggag tttccataat ttctcgcaca aaaggcaaac tgttgacaat ttggggatat
      301 atccccgtag aaatatttcc ttctgccata accgtggttc ccatatttac accaacaata
      361 gaggccaggc ttcccaggtt tcccaaggaa ctttgcctgc cgctttgcgg tagaataaca
      421 ctctgcgcgg tatattcaac cggcaaagtg aacgcaatga aaattcccaa cagaaaaaac
      481 aggccggtta cagccaagat aaatttgcgt ttcttccaga gttgaacgat aaggtctttt
      541 aaatcgatct catcggaatg gtttgtttct tgaagttgtt ctgtcatatg gagtagtcgt
      601 ttgttaaaag aatctgctga ttatcaacgc aagagaggca agtgtagttg acattcccag
      661 aatgttttga attcctaccg attcacgttc aggtctttcc ggtacgataa tctcgcaccc
      721 gggttctatt ttggcccacc ggccgctggc cactttccca ttcatataaa ttacaaacgg
      781 tttgtttttc atagccagtc gcgaataccc tcctgcctga cggacgtaac tttccaggct
      841 catgcgtttg ttgtaggtaa cggtgttcgg atacagtact ccaccagaaa tcttaaccgt
      901 accttcgtat tgaggaataa aaatctcgtc accttgacgc aggataatat catcatcgct
      961 tccgggtttc ttcagcgctt tctccaggtc aatcccgaca tattgtgtgc tgaagtccat
     1021 gttctcaata taggtggagt ctccttcggc ggtcgcccta gccaggagag catcgcgagt
     1081 ccttcttcgt tcggcttcgt ttaactggcg ggataatcgg gcccctttgg catacgcata
     1141 ttccgttaat ccccccgccc gctggataaa cgaagataac cgctcgtctt tttttatctt
     1201 ggcatactgc ccgtcaaaca acacttctcc acgtaaggta atattctgtt gtatttcgta
     1261 tcccggagag cggcgtatca caacttgatc gaaggggtga agtacaaatg atgtatcatt
     1321 cgatatgatt ataccgtctc ttaatgagaa acggaacgcc tctccggttt gggaagatga
     1381 agaggtgctg gctggattct taatccggcg ataaacatca atctgagccg tggaggccga
     1441 ttctcttaat cctccagctc tcaagacaac atcctcgagg tgcatgtttt ctgcataggg
     1501 ataattaccg ggagcgcgaa cttcccctcc tacatataca gtgaggctgt cttcgatgga
     1561 gaaaatagag ggtatatgca ggcgatcgtt cttttgcagc ggaatatcgg gtatgcggtt
     1621 gcttagcaat gcatccaggt cgaacgactg catggtctgc tttaagtctt cttgctcgcg
     1681 gtacaacaat gcgcggtaaa cataagcgtc gcccgttatt ccttgtgcta tcgataccag
     1741 gtcgcgcacc gtcctgatct gattcccaat agcatattgt cccggacgat acactgcccc
     1801 ggtaatttcc accatgttgg agaaccggtt gagtatggtg gatacggtta tgaaatcgcc
     1861 atcctgtatt gtgaaggagg caaagccggt ttgatttacg gtgaaggttt ctttctcgta
     1921 ttcgcccttc cggtctacct ggatgttgtt cttataggct tcgcccgtga aaccgccggc
     1981 aaaaccaaga atatcctgaa gcgtttcatc ggggcgcatc tcgtatttca tggggcgttt
     2041 tatttccccg gcgatctggg caagccctac gtacgggtcg accatcacga tatccccgtc
     2101 ctgcaaggaa atatcgttca tgttgttgcc tttgaggagg tattcgtaca aatcgaccgt
     2161 ggccgccacc ctgcctcccc ggaatacgcg gatgttgcgc aaacttccga tgggtgttat
     2221 gccgccggcg gcgtacaacg catgaaaagc cgatgagaag gacgtcagcg tatacgttcc
     2281 gggggtggtt acctctccca tcacattgac ttttatggtg cggatgttcc ccacggagag
     2341 ggcgagaaaa gtccggggtt ggctcgaatt gaggtcggaa tagatacccg aaaactcatt
     2401 gcggatacgg ccggttgcct ggtcgacgtt cattccgctg agctggatcc gccccagccc
     2461 cggcacagtg atgtacccgt cgggagtaat ggtgtagcgt acgttcagct ccgagtcgcc
     2521 ccacacgtcg ataatgacct cgtcgcccgg ccccaacaca taattagcgg gggtaggcat
     2581 gttcacgttg ggagcaaatg tcaggttttc ccgggagaaa aaatcctgtc cgaaaatacg
     2641 ttttccggga gggatggtgt cg
//
LOCUS       DAOSDR010000410         2646 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrimonas sp. isolate AMR_MDS_1826
            NODE_13596_length_2646_cov_4.814743, whole genome shotgun sequence.
ACCESSION   DAOSDR010000410 DAOSDR010000000
VERSION     DAOSDR010000410.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863355
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrimonas sp. (wastewater metagenome)
  ORGANISM  Petrimonas sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Dysgonomonadaceae; Petrimonas.
REFERENCE   1  (bases 1 to 2646)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2646)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:10:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,720
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 35
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2646
                     /organism="Petrimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13596_length_2646_cov_4.814743"
                     /isolate="AMR_MDS_1826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2023866"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            47..292
                     /locus_tag="PLI30_12625"
     CDS             47..292
                     /locus_tag="PLI30_12625"
                     /inference="COORDINATES: protein motif:HMM:NF017239.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PqqD family protein"
                     /protein_id="HOI80435.1"
                     /translation="MDIRKNIAVSENGFIFNPLTGDSFSVNQTGVFILRKMKEGDSNE
                     DIMKALQEEYELDTYTAEKDLYDFLSLLKSYQLTENE"
     gene            285..1334
                     /locus_tag="PLI30_12630"
     CDS             285..1334
                     /locus_tag="PLI30_12630"
                     /inference="COORDINATES: protein motif:HMM:NF014805.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HOI80436.1"
                     /translation="MSKRKITVGVSGLNNIDSPGPGIPVIRALKESSEFDVRIIGFSY
                     ETLEPGIYMHELVDKVYQLPLPTAGSSMLKERLQYIAGIEKIGVIIPNFDAELHNFIK
                     IQQQLKSELDIATFLPSHESFEERQKSELNKFGEKYNVRVPKSHMINQLSELKKLEDD
                     LDYPMVIKGRYYDAYVASSHEQASAGFYKIVSKWGYPVIVQEFVTGTEVNVTAIGDGY
                     GNCIGAVPMRKLYITDKGKAWSGISLEDDKLMEITRRVIENSKWRGGCELEFIKTKKD
                     EYYLLEMNPRFPAWVYLANGCGQNHAEALVKMALGEEVKPFAGYKSGKMFIRYSYDMI
                     VDITEFEKISTTGEM"
     gene            1337..>2646
                     /locus_tag="PLI30_12635"
     CDS             1337..>2646
                     /locus_tag="PLI30_12635"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF014803.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HOI80437.1"
                     /translation="MKPTYERPVIQKLNTGLLNKFGSRTEYTPVKQIDGVSVNNLMDQ
                     YGSPLFVISEKTIRQTYQRALKAFKMRYPKVQFAWSYKTNYLDAVCNVFHQEGSWAEV
                     VSGFEYQKALRNGVPGNLIIFNGPDKSVEDLKKAVENRSLIHIDHFDELYSLIEMLEG
                     TSYSARVAIRVNMDTGVYPQWDRFGFNYESGQAWNAIMKIVTQKNLELEGLHCHIGTY
                     MLTANAYAVAARKMCELAQATANTAGIKVKYLDMGGGFCSSNTLKGSYLQGVDVIPNF
                     DDYAEAIVNTLLHFGFKPDELPLLILETGRALIDEAGYLLGSVLATKRLADSRRATVL
                     DIGVNIMFTSFWYNHRVSPAQDFTQHAEEMALYGPLCMNIDCLRESVSLPLLKKGDRV
                     VVHEVGAYNMTQWMQFITLRPNVVMIDTTGKVHLIRKNETVETIV"
BASE COUNT          781 a          544 c          663 g          658 t
ORIGIN      
        1 cgttttacct caatacagga gtatcaagat taattaatca aagcctatgg atattagaaa
       61 aaacattgct gtcagcgaaa acggatttat ctttaatcct cttacgggag acagtttcag
      121 cgttaatcag acgggtgtat ttattctccg taaaatgaaa gagggtgaca gtaatgaaga
      181 tatcatgaag gcactccagg aggaatacga attagatact tacaccgccg aaaaggacct
      241 ttacgatttc ttgtctttac tgaaaagtta ccagctgacc gaaaatgagt aaaagaaaaa
      301 ttaccgtcgg ggtaagcgga ctcaacaata tagacagccc gggacccggg attcctgtca
      361 ttcgcgcatt gaaggaaagc agtgaattcg atgtgcgcat tatcggattc tcgtacgaga
      421 ccctggaacc gggaatttat atgcacgaac tggtggacaa agtgtatcag ttgccgttac
      481 ccactgcagg atcttcaatg ctgaaagaaa ggctgcaata tattgcgggc attgagaaaa
      541 taggtgtaat cattcccaat tttgatgcag aactgcacaa cttcatcaag atacaacagc
      601 aattgaaatc ggagctggac attgccacgt ttctgccttc tcacgaatcg tttgaagaac
      661 gacagaaatc ggaactgaac aaatttggcg aaaaatacaa cgtaagggtg ccgaaaagcc
      721 atatgataaa tcagctttcg gaattgaaaa aactcgaaga cgatttggat tatcccatgg
      781 ttatcaaagg caggtattac gacgcatacg ttgcttcttc tcacgaacag gcaagcgctg
      841 gtttttacaa gatagtgtcc aagtggggtt acccggtgat tgttcaggaa tttgtgaccg
      901 gaacggaagt gaacgtcacc gccattggtg atgggtatgg caactgtatc ggtgccgtgc
      961 cgatgcggaa gctttacatc accgacaaag gaaaagcgtg gtcgggcatt tcgctggaag
     1021 atgataagct gatggaaatt acacgccggg tgattgaaaa cagcaaatgg cgcggtggct
     1081 gcgagctgga gtttatcaaa accaagaaag atgaatatta tttgctggag atgaatccgc
     1141 gttttccagc ctgggtttac ctggcaaacg gatgcgggca aaaccacgcc gaagcattag
     1201 tgaaaatggc gctgggtgaa gaggtaaaac catttgccgg atataaaagc gggaaaatgt
     1261 tcatccgcta ttcttacgat atgattgtgg atattacgga atttgaaaaa atttcaacaa
     1321 cgggggaaat gtaaagatga aacctaccta tgaacgccct gtcattcaaa aactgaatac
     1381 gggcttactc aataaattcg gcagcagaac cgagtacaca cccgtaaaac agatcgatgg
     1441 tgtttccgta aacaacttga tggatcagta cggttcaccc ttgtttgtga tcagcgagaa
     1501 aaccatccgt caaacctatc aacgtgcgtt gaaggcgttt aaaatgcgtt acccgaaagt
     1561 gcagtttgcc tggagctata aaaccaacta tctcgatgcg gtctgcaacg tttttcatca
     1621 ggaaggaagc tgggcggaag tggtatcagg atttgaatac caaaaagcgc tacgcaacgg
     1681 agtgccgggt aaccttatca ttttcaacgg ccctgataaa tcggtggaag atttgaaaaa
     1741 ggctgtcgaa aaccgttcgc ttattcatat cgaccatttc gatgaactct attcacttat
     1801 cgagatgctg gaagggacct cctacagcgc acgcgtagcc attcgtgtaa atatggatac
     1861 gggtgtttat ccacaatggg acaggttcgg attcaattat gaatccggac aggcatggaa
     1921 tgctatcatg aagattgtga cacagaaaaa tctcgaactg gaaggcctgc actgtcacat
     1981 cggtacctat atgcttacgg caaacgcata tgcggttgct gcccggaaaa tgtgtgagtt
     2041 ggcacaagct acggcaaaca ctgccggtat taaagtcaag tacctggaca tgggtggcgg
     2101 attttgttct tcgaacacgc tgaagggctc ttacctgcag ggtgttgatg taattcccaa
     2161 ttttgacgat tatgccgagg ctattgtgaa cacccttttg cattttggtt tcaaacccga
     2221 tgaattaccc ttgttgatcc tcgaaacagg gcgggcgttg atcgacgagg ccgggtacct
     2281 gctggggtca gtcctggcta cgaaacgtct ggctgacagt aggcgggcaa ccgttctcga
     2341 tatcggtgta aacatcatgt ttacctcatt ctggtacaat catcgggtat cgcccgcaca
     2401 ggattttacg cagcatgccg aagaaatggc gctttatggc ccgttgtgta tgaatatcga
     2461 ttgccttcgt gaaagtgtga gcctgccttt gctgaagaag ggtgaccgtg tggtggttca
     2521 cgaagtaggt gcatacaaca tgacacaatg gatgcagttt atcacgcttc ggccgaatgt
     2581 agttatgata gacaccaccg ggaaggtgca tttaatccgg aaaaacgaaa ccgtggaaac
     2641 tatcgt
//
LOCUS       DAOSDR010000411         2644 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrimonas sp. isolate AMR_MDS_1826
            NODE_13609_length_2644_cov_4.242178, whole genome shotgun sequence.
ACCESSION   DAOSDR010000411 DAOSDR010000000
VERSION     DAOSDR010000411.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863355
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrimonas sp. (wastewater metagenome)
  ORGANISM  Petrimonas sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Dysgonomonadaceae; Petrimonas.
REFERENCE   1  (bases 1 to 2644)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2644)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:10:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,720
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 35
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2644
                     /organism="Petrimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13609_length_2644_cov_4.242178"
                     /isolate="AMR_MDS_1826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2023866"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..513
                     /locus_tag="PLI30_12640"
     CDS             <1..513
                     /locus_tag="PLI30_12640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004308325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_process: GO:0055070 - copper ion homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HOI80438.1"
                     /translation="IEMCRKLGVDGIVIGCLTEEGDVDMERCRELVEAAEKMDVTFHR
                     AFDQCRDPFISLEDIISLGCSRILTSGQQPKAEQGVDLLKKLVQQAGERIIIMPGSGI
                     NENNIAKIARETGAVEFHLSAREAVESKMKFRNSAVSMGGTNITINEFEQLITSAQKV
                     KNTWTVLNIK"
     gene            515..>2644
                     /locus_tag="PLI30_12645"
     CDS             515..>2644
                     /locus_tag="PLI30_12645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010537719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 20 glycosylhydrolase"
                     /protein_id="HOI80439.1"
                     /translation="MKKFKILSVLIAVIALLLSCSPKEKKSIESANYQVIPLPSEIVT
                     SEGNPFVLSSSVKIVFPEGNEKMQRNAEFLAEFLNISTGIKPDITSTAPDKNAIILGI
                     GLQNPNKEAYEIAVGENSITITGASEAAVFYGIQTLRKSVLAGTKHVVFQPVTIKDEP
                     RFSYRGMMLDVARHFQSVDFVKKYIDILALHNINRFHWHLTDDQGWRIEIKAYPKLTV
                     IGSQRKQTVVGRNTGEYDGKPHGGFYTQEEIRDIVKYAQDRYITVIPEIDLPGHMLAA
                     LAAYPELGCTGGPYEVAGSWGVFDDVLCPGKEETFTFLESVLSEVIELFPSEYIHIGG
                     DECPKVRWEECPDCQTRIKELNLKDKEGHKAEHYLQSYVTARIEKFLNDKGKSIIGWD
                     EILEGELAPNATVMSWRGMEGGIQAAQMGHDVIMTPTTYCYFDYYQTQNTDEEPLAIG
                     GYVPIEKVYSFEPAPDILTDGQKARILGLQANLWTEYIETPDYVEYMIMPRIAALSEV
                     QWVKPEKKNYEAFLTRLPGLLNLYGKLGYNYATHVFDVQAKMIPNFETNSLDVELSTI
                     DNAPVYYTLDGTVPTVSSTKYDGKFSIRENTEIKAMAIREGGNTSKVLSEKINASKAS
                     YKPVTLLTTPDPNYRYTGEGMLVDGLFGNSTNYKTGKWMGFKGENIVAIIDMLEPTEI
                     STAQIRNCVVTGDWIFDASEIVLESSDN"
BASE COUNT          826 a          568 c          630 g          620 t
ORIGIN      
        1 atcgaaatgt gtcgcaaact gggtgttgac gggattgtaa tcggctgtct cacggaagaa
       61 ggtgatgtag atatggaacg ctgtagggag ttagtggaag cagcagaaaa aatggatgtg
      121 acctttcatc gggcattcga tcaatgccgg gatccgttta taagccttga agacattatt
      181 tcgctgggtt gcagcagaat tctcacgtcg ggtcagcaac ccaaggcaga acaaggggta
      241 gatttattga aaaaactggt tcaacaagcc ggagagcgca tcatcattat gccgggaagc
      301 ggaattaacg aaaataatat tgcaaagatc gctcgggaaa ccggtgcggt tgagtttcat
      361 ctttctgccc gggaagcggt ggaaagtaag atgaagttca gaaactcagc cgtttcaatg
      421 gggggaacaa acatcacgat caacgaattt gaacaattga ttaccagtgc ccaaaaagta
      481 aaaaatacat ggactgtttt aaatataaaa tgaaatgaaa aaattcaaga ttctctctgt
      541 ccttattgca gtaatcgccc tattgctctc ttgttcaccg aaggaaaaga aaagcataga
      601 atctgccaat taccaggtta ttccgttgcc atcggaaatc gtgacatccg aaggaaatcc
      661 ttttgttttg tcctcatcag taaaaatagt atttccggag gggaatgaaa agatgcagcg
      721 taacgcggaa tttttagcgg aatttttgaa tatatccaca ggtataaaac cggatattac
      781 ctcaacagca ccggacaaga acgctatcat tttgggcatc ggcctccaaa atccaaataa
      841 agaagcctat gaaatagcag tcggtgaaaa cagcataact ataacgggcg caagtgaagc
      901 cgctgttttt tatgggatac agacgttgcg caagtctgtt ttggcaggca caaaacacgt
      961 tgttttccaa cctgtgacaa tcaaggacga gcctcggttc tcctaccgcg gcatgatgtt
     1021 ggatgtggcg cgccatttcc aatcggtaga ttttgtaaaa aaatacatcg atatcctggc
     1081 attgcacaac atcaatcgct ttcactggca cctgaccgat gaccaaggat ggagaatcga
     1141 gataaaagcc tatccgaagt taacggtaat cgggtctcag cgcaaacaaa ctgttgtggg
     1201 cagaaacaca ggggagtatg atggaaaacc tcacggcggt ttttatacac aggaagaaat
     1261 tagagacatc gttaaatatg cccaagatcg gtacatcacc gttattcccg aaattgatct
     1321 tccgggacat atgctggcag cccttgccgc atatcccgag ctaggctgca caggaggtcc
     1381 gtacgaagtt gcgggttcgt ggggagtttt tgacgatgtt ctttgtccgg gaaaagaaga
     1441 aactttcact ttcctggaaa gtgtgctttc ggaagtaatt gaacttttcc cgtccgaata
     1501 catccacatc ggtggcgatg aatgtcccaa ggtccgctgg gaagagtgtc ccgactgcca
     1561 gactcgtata aaagaattaa acctcaaaga caaagaagga cataaggcag aacattacct
     1621 gcaaagctac gtgactgccc gcatcgaaaa attcctgaac gataaaggga aaagtattat
     1681 cggttgggat gagattttgg aaggggaact ggctccaaac gcgacagtca tgtcatggcg
     1741 cggaatggaa ggaggtatcc aggctgctca aatggggcac gatgtaatta tgacgccaac
     1801 cacctattgc tatttcgact attaccagac ccagaacact gatgaagagc cgttggcaat
     1861 cggcggatat gtccctatcg aaaaagtgta cagctttgaa ccggcacctg atattttgac
     1921 agacggacaa aaagcacgta tcctggggct gcaagctaac ttgtggacag aatacattga
     1981 aacgccggat tacgtggaat acatgataat gccccgtatt gccgcgctat ccgaagtaca
     2041 atgggtaaag cccgaaaaga agaattacga agcattcctt acccggctac ccggactgct
     2101 caacctttac ggcaagctag gttacaacta tgcaacacac gtttttgacg tacaggcaaa
     2161 aatgattccc aatttcgaaa ccaattcgct cgatgtggaa ttatcaacca ttgacaatgc
     2221 accggtatat tatacgctag acggtacggt tcccaccgtt tcgtcgacaa aatacgacgg
     2281 taaattctcc atcagggaga atactgaaat taaagcaatg gctattcgcg aaggtgggaa
     2341 cacaagcaaa gttctctcgg aaaaaattaa tgcaagcaaa gccagctaca aacccgtaac
     2401 actactgact acaccagacc cgaactacag gtataccggt gaaggcatgc tagtcgacgg
     2461 ccttttcggg aacagcacga attacaaaac cggtaaatgg atgggtttca agggtgaaaa
     2521 tatagtggcc ataatcgata tgctcgaacc cacagaaata tcaaccgctc aaattcgtaa
     2581 ctgcgtggta accggcgact ggatctttga tgcatcggaa attgttctcg aatcctctga
     2641 taac
//
LOCUS       DAOSDR010000412         2637 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrimonas sp. isolate AMR_MDS_1826
            NODE_13660_length_2637_cov_2.674284, whole genome shotgun sequence.
ACCESSION   DAOSDR010000412 DAOSDR010000000
VERSION     DAOSDR010000412.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863355
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrimonas sp. (wastewater metagenome)
  ORGANISM  Petrimonas sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Dysgonomonadaceae; Petrimonas.
REFERENCE   1  (bases 1 to 2637)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2637)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:10:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,720
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 35
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2637
                     /organism="Petrimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13660_length_2637_cov_2.674284"
                     /isolate="AMR_MDS_1826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2023866"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..710)
                     /locus_tag="PLI30_12650"
     CDS             complement(<1..710)
                     /locus_tag="PLI30_12650"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOI80440.1"
                     /translation="MFKTALKLIFRNWWRNKTFTLISILSLTVGIACTALLISFVSYE
                     YGIEKNNPNRNKLVWVMQDMPSNPGEKVAYMSSDVPKQLQEKYPEMEGFLQLNSFGMK
                     YIEVNNQHLEPMEILNVDASFPEFFPFELLYGSWNAFHNPQSIVLSEKQTQKFFGNEN
                     ALGKQITVCKEDGFGSEKIIVYTVGAVTKSRTQSAIIFDGLVCDPENNWGGPTLLMMP
                     VHTDRSQFEEKVKKDRIPT"
     gene            complement(759..>2637)
                     /locus_tag="PLI30_12655"
     CDS             complement(759..>2637)
                     /locus_tag="PLI30_12655"
                     /inference="COORDINATES: protein motif:HMM:NF014717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HOI80441.1"
                     /translation="VKAVICNPPANSIFKRVGYLSDSDALFLYTKVDDVVKWHDFNDT
                     RAFFQLKKNTDIKKFNEKLRTFAIDNNYNDDLLFGMVPLKSVRQTISNPFDKVTFDIK
                     YIRTFILSALLLIFAAFFNYLNILINTMFTRVREMNLRRVNGASTLNIFGQIVIEMLL
                     LIVVVLLFSFCALELTIPVFEKTFSTIIITGKIYRILLVTICAVSTMLYLTAFVFLSR
                     FIRKTSLRQSTPTKNKFSSNRISLLLQLAISLFFILSAFVLYRQVHFMHNADWGFNKN
                     HLLQIEMKLNERIPFMENVKKLPMVKNVINTNFFTIIPNMENMGGTRAMDVEWEGKPM
                     DYNPVFQTFDVEMNFVTDIGLTLQMGRDFAEQDLVGRWKSDKILINETAQRIMEMENP
                     IGKKITVPANWSNEQGRGKEEFEIIGLVKDFHTIALQSEIPPLLIKGMSKANGGYFNY
                     VRVVSGTENDAIKAINELVLKHQPDDENEELVKTVDSILADLSKTEQDVMNLFFTVAA
                     LCVLIAVFGIYSVSQRETQRRRKEIAIRKTAGAKTREIMAMFIREYLIITLAACAGAF
                     PLAGLFMHRWLQGFAYRISISWWMFAVVSLVVAVIVLLTIFSQVNRASNQNPAEVVKS
                     E"
BASE COUNT          730 a          579 c          535 g          793 t
ORIGIN      
        1 gtcggtatcc ggtctttctt caccttttct tcaaattggg agcggtctgt atgcaccggc
       61 atcatcaaca gtgtgggacc gccccagttg ttttcggggt cgcagacaag cccgtcgaag
      121 ataatggccg actgcgtgcg gctttttgtt actgccccaa ccgtgtaaac gattattttt
      181 tccgagccga aaccgtcctc cttacacacc gtaatctgtt ttcccagagc gttttcgttg
      241 ccgaaaaact tttgtgtctg cttttcgcta agaacgatcg actgcggatt gtggaaagcg
      301 ttccatgaac cgtacagcag ttcgaacgga aagaattccg gaaaagaggc atccacgttg
      361 agaatttcca ttggctcaag atgttggttg ttcacctcga tatacttcat gccaaaactg
      421 ttcagttgca ggaacccttc catttcggga tacttctctt gcagttgttt gggaacatcg
      481 ctgctcatat acgccacttt ttctccgggg ttggacggca tatcctgcat cacccacacc
      541 agtttattgc ggttgggatt gttcttctcg attccgtatt cgtagctgac gaaagaaatc
      601 agcagagccg tgcaagcaat acccacggta aggctgagga tggaaataag tgtgaacgtt
      661 ttgttgcgcc accaatttcg gaatattaat ttaagtgccg ttttgaacat atacttgttt
      721 tttagatgat aaaagttaag agtcaagact ttagactttt attccgattt caccacctcc
      781 gccggatttt gatttgacgc tctgtttacc tgactaaaaa ttgtaagcag cacgataacc
      841 gccacaacca aactcaccac cgcaaacatc caccacgaaa tggaaatgcg ataggcaaac
      901 ccttgcagcc aacggtgcat aaataatccg gccaacggga acgccccggc gcacgctgca
      961 agcgtaataa tcaggtactc gcgaatgaac atcgccatta tctcgcgcgt tttcgctccc
     1021 gccgttttgc ggatggcaat ctccttgcgg cgtcgttgcg tttcgcgctg cgacacggaa
     1081 tagatgccga aaacggcaat caacacacac aaagcggcta cagtgaagaa caaattcatt
     1141 acatcttgtt cggttttgct taaatcggcc aagatactgt ccactgtttt cactaattct
     1201 tcgttttcgt cgtcgggttg gtgttttaac acaagttcat tgatggcttt aatggcatcg
     1261 ttttctgttc cggaaacaac acgcacgtaa ttaaagtatc caccattggc tttactcatc
     1321 cctttaatta ataaaggagg aatctcactt tgcagggcaa tggtatgaaa gtcttttact
     1381 aaacctataa tctcaaattc ttcttttccg cgtccttgct cgttagacca attggcgggg
     1441 acggttattt tctttcctat gggattttcc atttccatta ttcgttgagc ggtttcattg
     1501 atgagtattt tatcggattt ccacctgccg actaaatcct gttcggcaaa atcccgcccc
     1561 atttgtaaag taagccctat atccgtaaca aaattcattt ccacatcaaa agtttgaaaa
     1621 acaggattat aatccatcgg cttgccttcc cattcaacat ccatcgcgcg tgttcctccc
     1681 atattttcca tattcggaat aatggtgaaa aaattggtat tgatgacgtt tttcaccatt
     1741 ggcagttttt ttacattttc cataaaggga attctttcgt tcaacttcat ttctatttgc
     1801 agaagatgat ttttgttgaa tccccaatcg gcattgtgca taaaatgcac ttgacgataa
     1861 agaacaaacg cgcttaggat aaaaaacagg ctgatagcaa gctgtaataa caagctaatt
     1921 ctgttggatg agaatttgtt ttttgtcggg gtagattgtc ttaacgaagt ttttcggata
     1981 aaccgggata agaacacaaa tgcagtaagg taaagcattg tgctaactgc acagatagta
     2041 acgagcaaaa tccgatatat tttaccggta atgattatgg tggaaaaagt tttttcgaaa
     2101 accggaatag tcaactccag tgcgcaaaac gaaaaaagca gcacaacgac aatcaacaga
     2161 agcatttcta tgactatctg cccgaaaata ttgagtgtgg atgccccgtt tacccggcgc
     2221 aaattcattt cccttacgcg tgtaaacatt gtattgatga gaatattgag gtagttgaaa
     2281 aacgcggcaa aaatcaataa cagcgccgaa agaataaacg tgcggatata ttttatatcg
     2341 aatgtcactt tatcgaacgg attgctgatc gtttgtctaa cggattttag gggaaccata
     2401 ccaaataaca gatcatcatt gtaattattg tcgatggcaa aagtccttaa tttttcgttg
     2461 aattttttga tatctgtatt cttttttaac tggaaaaaag ctcttgtatc attaaaatcg
     2521 tgccatttta caacatcgtc gacttttgta tataagaata gtgcatccga atcggataag
     2581 tatcccactc ttttgaaaat gctgtttgcc ggagggttgc aaataaccgc ttttacg
//
LOCUS       DAOSDR010000413         2635 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrimonas sp. isolate AMR_MDS_1826
            NODE_13673_length_2635_cov_3.007364, whole genome shotgun sequence.
ACCESSION   DAOSDR010000413 DAOSDR010000000
VERSION     DAOSDR010000413.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863355
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrimonas sp. (wastewater metagenome)
  ORGANISM  Petrimonas sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Dysgonomonadaceae; Petrimonas.
REFERENCE   1  (bases 1 to 2635)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2635)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:10:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,720
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 35
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2635
                     /organism="Petrimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13673_length_2635_cov_3.007364"
                     /isolate="AMR_MDS_1826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2023866"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..475
                     /locus_tag="PLI30_12660"
     CDS             <1..475
                     /locus_tag="PLI30_12660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006949924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
                     /protein_id="HOI80442.1"
                     /translation="IARYVGELAEIAKPFHLRVEGPVDMGEKAAQIDALRIIRQTMEK
                     MEITAEIVADEWCNTLQDVIDFSENKAGHMAQIKTPDLGGINNSIEAVLYCKQHHMGA
                     YLGGTCNETNRSAEIGAHIAMATSPVQYLAKPGMGVDEGYMIVYNEMSRILALNH"
     gene            506..1864
                     /locus_tag="PLI30_12665"
     CDS             506..1864
                     /locus_tag="PLI30_12665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006744492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyclic terpene utilization AtuA family protein"
                     /protein_id="HOI80443.1"
                     /translation="MKELRILSPTAILGYGFPMESFEEGMKRKPHVIAVDAGSTDPGP
                     YYLGAGKSFTDRNSVKRDLEIMIPAALEAGIPVIVGTAGGSGARPHVEFAVDIVKEIA
                     REKRLSFKLAIIQSEFDKELVKENLRKGNISPLYPAPEITGADVDESVHIVAQMGEEP
                     FIEALENGANVILAGRSYDPSVFSALAIKNGFNKGLAIHLGKILECAAIAALPGSGSD
                     CMFGYLHEDNFVLEPLSPLRKCTTLSVAAHTLYEKTNPYVLPGPGGAINLHESKFEQI
                     DDNKVRVSGSRFVPTEEYFVKLEGVKRVGYRTISCAGVKDPIMITKIDSITQSVKERV
                     QNNFETYGITDFFLDFKIYGRNGVMGMFPDAPQRTGDELLIIIEAVAPTQEQADTICG
                     FARSTMLHFGYEGRIATAGNLAFPFSPSDCKMGEVYEFNVYHLMKVEDPKKLFPIEYI
                     HF"
     gene            1966..2283
                     /locus_tag="PLI30_12670"
     CDS             1966..2283
                     /locus_tag="PLI30_12670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006744493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4387 domain-containing protein"
                     /protein_id="HOI80444.1"
                     /translation="MKYNLTDVASVIRSKNSGPYELTFDIIFKEMDMYQQVKSAKVFT
                     AQMFALLYHIPESQIISLVHFDPAKAVKITVVRPIPSGALGETDVYGAQQHAPLMRMT
                     FEL"
     gene            2280..>2635
                     /locus_tag="PLI30_12675"
     CDS             2280..>2635
                     /locus_tag="PLI30_12675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006744494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine-tRNA synthetase second additional
                     domain-containing protein"
                     /protein_id="HOI80445.1"
                     /translation="MRALIQEYMVSSQYFAPRGKERLMFLGEQIAQRHLSYNDRLIGI
                     VGDAGSGKSSLIKGMFPGLELANDDDVIHSRKIMQVRSLSDDWHDSTTFHIDMRFQTA
                     FTQMFEIVAWVQDLLA"
BASE COUNT          720 a          639 c          673 g          603 t
ORIGIN      
        1 cattgcccgt tatgtggggg agctggcaga gatagcaaaa ccgtttcacc tgcgcgtcga
       61 aggtccggtg gatatgggag agaaagcggc tcagatcgac gcgctccgca ttatccgcca
      121 aacgatggaa aaaatggaaa ttacggcaga gattgttgcc gacgagtggt gtaatacgct
      181 ccaggatgtc atcgacttct ccgaaaacaa ggctggacac atggcgcaaa tcaaaacacc
      241 cgatctggga ggaatcaaca acagcataga ggctgtgctg tactgcaagc aacaccatat
      301 gggcgcctac ctgggcggaa cctgcaacga gaccaaccgt tccgccgaaa ttggagcaca
      361 cattgccatg gctacttccc ccgttcaata ccttgccaaa cccggtatgg gcgtggatga
      421 agggtatatg atcgtttaca acgaaatgag ccggatactg gcacttaacc attaattcac
      481 ccaaaaatta ctaaaagctg aaaaaatgaa agaactgaga atactttcgc ccacagccat
      541 cctcggatac ggatttccga tggaatcgtt tgaggaagga atgaaacgca aaccgcacgt
      601 tattgcggta gatgccgggt caaccgatcc gggtccctat tacctgggtg cagggaaatc
      661 gtttaccgac cggaattcgg tgaaacgcga ccttgagatt atgatacctg ccgcactgga
      721 agccggaatt ccggttatcg taggtacggc cggcggttcg ggtgcgcgtc ctcacgtgga
      781 gtttgcggtg gatatcgtga aggagatcgc ccgagagaaa aggctgtcgt ttaaactcgc
      841 cattatacaa tcggagttcg ataaggaatt ggtaaaagag aacctgcgca aagggaacat
      901 ttcaccgctt tatccggctc ccgaaataac cggagccgat gtggacgaat cggtgcatat
      961 cgtagcccag atgggcgaag agccgttcat tgaagccttg gagaacggag ccaatgttat
     1021 acttgccgga cggagctacg acccgtcggt tttttcggcg ctcgccatta aaaacggttt
     1081 caacaaagga ttggccattc atctgggtaa aatcttggag tgcgctgcca ttgccgccct
     1141 gcccggtagc ggaagtgact gtatgttcgg ctatttgcac gaagacaatt ttgttctcga
     1201 accgctctcg cccctgcgta aatgcaccac gctctcggta gctgcacaca cgctgtacga
     1261 aaaaaccaat ccctatgttc tacccgggcc ggggggagcc atcaatttgc acgaatcaaa
     1321 attcgagcag atagacgaca ataaagtacg cgtaagcgga agccgttttg taccgaccga
     1381 ggagtatttt gtgaaactgg aaggggttaa gcgtgtggga tacagaacca tttcgtgcgc
     1441 cggagtgaag gacccgatca tgattacgaa aatagacagc attacccaaa gcgtaaaaga
     1501 acgtgtgcaa aacaactttg aaacgtacgg aattaccgac ttcttcctcg atttcaagat
     1561 ttacgggcga aacggtgtga tgggcatgtt ccccgatgcg ccacaacgta ccggcgacga
     1621 gctcctgatc atcatcgaag ctgtcgcacc cacacaagag caagccgata ccatttgcgg
     1681 gttcgcacgc agtactatgc tgcactttgg ttacgaaggc cggatagcca ctgccggaaa
     1741 cctggcattt ccgttttctc cgtccgattg caagatgggt gaggtgtacg agttcaatgt
     1801 gtaccatttg atgaaagtgg aggatcccaa aaaacttttc ccgatagaat atattcactt
     1861 ctaatagatt gaaaaagatt gattaagatt gaaaacggtc agccaactat caatctcagt
     1921 caatctcagt caacctcatt cgatctcaat taaacttcaa acgaaatgaa atacaaccta
     1981 accgacgtag cttccgttat ccggagcaaa aattcagggc cttacgaact cacgttcgac
     2041 attattttca aggagatgga catgtatcag caagttaaat cggcaaaagt gtttaccgcg
     2101 caaatgtttg ctcttcttta ccacattccc gaatcgcaaa tcattagcct ggtacacttc
     2161 gatccggcca aggcggtgaa aattacggtt gtgcgcccca tcccctccgg ggcattgggt
     2221 gagaccgatg tgtacggagc gcaacagcat gcgccgttga tgaggatgac attcgaatta
     2281 tgagggcact gattcaggaa tacatggtgt cgtcgcaata ttttgcgccg cgcggaaaag
     2341 aacggttgat gtttctgggc gaacagattg ctcaacggca cctttcctac aacgatcggc
     2401 tgataggcat cgtgggtgat gccggctctg gaaaatcatc gctgatcaag ggaatgtttc
     2461 ccggcttgga actagcgaac gacgatgacg ttatccattc ccggaaaata atgcaggtgc
     2521 gctcgctttc cgacgattgg cacgattcca ctacctttca tatcgatatg cgtttccaaa
     2581 ccgcttttac ccagatgttt gaaatcgtgg cgtgggtaca agacctgttg gctca
//
LOCUS       DAOSDR010000414         2623 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrimonas sp. isolate AMR_MDS_1826
            NODE_13753_length_2623_cov_2.866044, whole genome shotgun sequence.
ACCESSION   DAOSDR010000414 DAOSDR010000000
VERSION     DAOSDR010000414.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863355
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrimonas sp. (wastewater metagenome)
  ORGANISM  Petrimonas sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Dysgonomonadaceae; Petrimonas.
REFERENCE   1  (bases 1 to 2623)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2623)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:10:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,720
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 35
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2623
                     /organism="Petrimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13753_length_2623_cov_2.866044"
                     /isolate="AMR_MDS_1826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2023866"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(249..2135)
                     /locus_tag="PLI30_12680"
     CDS             complement(249..2135)
                     /locus_tag="PLI30_12680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019540932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GxGYxYP family putative glycoside hydrolase"
                     /protein_id="HOI80446.1"
                     /translation="MKNFIYSSLCLLLSFSCNVPNSESIGLFDLKYSLHYDLNDPQLV
                     ESMWDDIHATATLQGIVNRDAPRLFINYVVQSDIEVDTYWWHKYRQPGKWLHDKDTVV
                     YHDIVELIEGYKHFINGVVLYDSSIASTSNVASAIAGIEDLIAVRYDPAPGSLYSRVV
                     LAGPKLKVKERLINQDGTPLFTGKGTIPGTNRVSTGSLKNDPYIWFIERYMKTNQCSG
                     EFGAYYIDQRWRMNPTATVVNHHTLSNHDFFVSKKAFFFDLSPWGDEPATDDTDQAVG
                     TDLATLKEFLSEAYRINKGEKMCYIGGFPSWAFKYTQHAGGMHRDVATEWEFSKLIGA
                     YNAFKDADAIGYGALANASFWTHFPLKEKYPQKKVSIDELKQRGYLDDEGKVDFKGRD
                     FIIFYVGDYDASSWVAQRTPTIWDNKSRGKVPMMWAISPVLEKRVPMALDYYRETATP
                     NDYFVAADNGAGYLMPGMLQEPREISGLKSGLDAWAKHCTPYYNRWDLTITGFVIDGT
                     APGLTKEGLDCYASFSPDGIVPQKVPLTVLHGNMPVLRSDEDIQQNPKEAAQRVAQRV
                     SERPIPFHWFRNILKTPDWYVTVTEELGKLNPDIELLDAPTFFELYRIWLHQNPDAAT
                     GKIK"
     gene            complement(2132..>2623)
                     /locus_tag="PLI30_12685"
     CDS             complement(2132..>2623)
                     /locus_tag="PLI30_12685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004305222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 92 protein"
                     /protein_id="HOI80447.1"
                     /translation="GNYAHGNQPIQHMTYLYNYSGEPWKAQYWVREVMNRLYWPTPDG
                     YCGDEDNGQTSAWYVFSALGFYPVCPGTDQYVVGSPLFKKATIHLENGKNVVINAPHN
                     NAENRFIKRVELKGETYTKNYLKHTELMEGITIEIEMDDAPNKQRGTKDSDFPYSYSN
                     KIK"
BASE COUNT          658 a          617 c          557 g          791 t
ORIGIN      
        1 tgtttattga gccgataata caactctcga ccgactgccc ctgcttgggc ataattacta
       61 aatttgccat tgattactaa ttcaactatc tgtttttaac ttaaaatttt gctttctagg
      121 atttattttg tttcattagt gtccccccct ctttcacaac agaacgtccc tttttataaa
      181 gtcacaataa gggtaatcta ttttccgaag aaacaatccg tagttatatt tatctataaa
      241 tccgtgtgtt atttaatttt tccggtggct gcatctgggt tttggtgtaa ccaaatccgg
      301 tacaattcaa aaaaggtagg agcatctaat aattcaatat cggggtttaa tttccccaat
      361 tcttccgtaa ctgtcacgta ccaatcgggt gtttttaaaa tattccggaa ccaatgaaag
      421 ggtataggtc gctcagaaac cctctgggca acacgttgag cagcttcttt cggattttgt
      481 tggatatctt catcagatcg taatacaggc atatttccgt gcagcacggt caacggcacc
      541 ttttggggca cgataccatc tgggctaaat gaggcataac aatccaatcc ttcttttgtg
      601 agaccaggag cagtgccatc aatgacaaag ccggtaatgg taagatccca tcggttgtag
      661 tagggagtgc aatgtttcgc ccatgcatcc aaacccgatt tcaagcccga tatttcccgg
      721 ggttcctgca acatcccggg catcaggtaa cccgcaccat tgtcggctgc cacgaaataa
      781 tcattgggcg ttgccgtctc acgataataa tccagagcca tcggcaccct cttttccaac
      841 actgggctaa ttgcccacat cattggtact tttcccctgc ttttattgtc ccagatggta
      901 ggtgtgcgct gggccaccca ggaggatgca tcataatccc ctacgtagaa tatgataaaa
      961 tctcttcctt tgaaatccac ctttccttca tcatcgagat accccctctg tttcaactcg
     1021 tcaatcgaca cctttttttg cggatatttc tctttcaaag ggaaatgggt ccagaaagag
     1081 gcattggcca aagcaccata tccaatggcg tcggcatctt taaaggcatt gtatgccccg
     1141 ataagcttgg aaaactccca ttctgtagcc acatcacggt gcatgcctcc cgcatgttgt
     1201 gtatatttaa aagcccacga aggaaatcca cctatgtagc acatcttctc tcctttattt
     1261 attctgtaag cttcggaaag aaattctttc agtgttgcca ggtcagttcc cacagcctga
     1321 tcggtgtcgt cagtagccgg ctcatccccc cagggagaga ggtcgaagaa gaaggctttc
     1381 ttgcttacaa aaaagtcatg attactcaag gtatggtgat tcacaaccgt tgcagtgggg
     1441 ttcatacgcc atctctggtc aatgtaatag gctccaaact caccggaaca ttgattggtt
     1501 ttcatgtatc tctcaataaa ccagatgtat ggatcgtttt tcagggagcc ggtcgagacc
     1561 ctgttggtac cgggtatcgt ccctttccct gtgaagagcg gtgtgccatc ctgattgatc
     1621 aacctctcct tcactttcaa ttttggtcct gcgagcacaa cgcgcgaata gagacttccc
     1681 ggtgcaggat catagcgaac cgctatcagg tcctcgattc ctgcaatggc tgaggcaacg
     1741 ttactggttg atgctatcga agaatcgtag agaacaacgc cattgataaa atgtttgtaa
     1801 ccttcgatca gttccacgat atcgtgatag actaccgtat ctttatcgtg cagccatttg
     1861 ccaggctgac ggtatttatg ccaccagtaa gtgtcaacct ctatatcgga ttggaccaca
     1921 tagttgataa acagacgggg cgcatcccgg ttgacaattc cctgtagggt ggctgttgcg
     1981 tgaatatcat cccacatgct ctcaaccagt tgagggtcgt ttaaatcata atgcaggctg
     2041 tatttcaggt caaacagtcc aattgattcg gaattgggaa cattgcatga gaaagataac
     2101 agcaggcaaa gtgagctata aataaaattt ttcatttgat tttgtttgaa tatgagtaag
     2161 ggaagtctga atcttttgta cccctttgtt tgtttggggc atcatccatt tcaatctcta
     2221 ttgttatacc ttccatcagt tcagtatgtt tcaggtaatt ttttgtgtag gtctctcctt
     2281 taagttccac cctttttata aagcggtttt cggcgttgtt atggggtgca ttgatcacca
     2341 cattctttcc gttttccagg tgtatcgttg cttttttaaa gagagggctt cccactacat
     2401 actgatcggt tccgggacaa acagggtaga agccgagggc actaaacaca taccatgccg
     2461 acgtttgtcc gttgtcttcg tcaccacagt atccgtccgg tgtcggccaa tacagacggt
     2521 tcatcacttc acgcacccaa tattgggctt tccagggttc accggagtaa ttatagagat
     2581 aggtcatgtg ttgaatcggt tggttgccat gtgcgtagtt tcc
//
LOCUS       DAOSDR010000415         2611 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrimonas sp. isolate AMR_MDS_1826
            NODE_13829_length_2611_cov_3.756651, whole genome shotgun sequence.
ACCESSION   DAOSDR010000415 DAOSDR010000000
VERSION     DAOSDR010000415.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863355
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrimonas sp. (wastewater metagenome)
  ORGANISM  Petrimonas sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Dysgonomonadaceae; Petrimonas.
REFERENCE   1  (bases 1 to 2611)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2611)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:10:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,720
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 35
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2611
                     /organism="Petrimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13829_length_2611_cov_3.756651"
                     /isolate="AMR_MDS_1826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2023866"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..540
                     /locus_tag="PLI30_12690"
     CDS             <1..540
                     /locus_tag="PLI30_12690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HOI80448.1"
                     /translation="YDVLIGVNLLREGLDLPEVSLVAVLDADKEGFLRSHRSLTQTAG
                     RAARNVNGKVIFYADKITDSMQTTIDETNRRREKQIKYNEQHGITPQQIIKARSSVLN
                     KEYTVTAEARAYVEPDYYSMASEPLPEYATADDLKTKIEQTRKAMLSAAKKLEFLEAA
                     RLRDELIKLEDELEEKSKC"
     gene            600..2264
                     /locus_tag="PLI30_12695"
     CDS             600..2264
                     /locus_tag="PLI30_12695"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008022575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004329 - formate-tetrahydrofolate ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HOI80449.1"
                     /translation="MKSDIQIARETPLKKIKEVAENIGIDRDEVHNYGPYMAKVPLRL
                     KNQKKIDQSNLVLVTAITPTKAGIGKTTVSIGLALGLNKIGKKAIVALREPSLGPVFG
                     MKGGAAGGGYAQVLPMENINLHFTGDFHAVTSAHNTISALLDNYIFRVQGTPHEIKEV
                     LWKRVLDVNDRSLRYIVTGLGPGNGVPLEGGFDITAASELMAILCLAEDEEDLRRRIE
                     NILLGYTREDKPFTVKDLGVAGAITVLMKDAIHPNLVQTTENTAAFVHGGPFANIAHG
                     CNSVSATKMAMSYGEYVITEAGFGADLGAEKFFNIKCRKSGLQPKLTVIVVTAQGLKM
                     HGGTPETDIRQPDMEGLKKGLENLSKHLENLASFGQTVVVAFNKYAFDTQDEIDAVAE
                     FCRQRGVRFAVNNAFVEGGQGAVELANAVVEAIEKEPSQPLQFTYSDEDSIRTKLEKI
                     ALNIYGARDVVFNEKALRRLKRIDDTPLAKFPVCIAKTQFSFSADPKEYGVARGFRFK
                     INDIVINQGSEFIVAIAGEMMRMPGLPADPQAKRIDIVNGKVEGLS"
     gene            2377..>2611
                     /locus_tag="PLI30_12700"
     CDS             2377..>2611
                     /locus_tag="PLI30_12700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019540454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI80450.1"
                     /translation="MIAVIKGDIVSSRKVENSEKWLLPLKSLFNEWGKSPGQWEIVWG
                     DSFQLEVDRPEEALKKALRVKALIKKIVSDETNK"
BASE COUNT          779 a          523 c          662 g          647 t
ORIGIN      
        1 tacgacgtgc tcatcggggt taacctgctt cgcgaaggat tggatcttcc cgaggtttcg
       61 cttgtcgctg tcctggatgc cgacaaggaa ggtttcttac ggtcgcatcg ttccctgaca
      121 caaactgcgg ggcgtgccgc ccgaaacgtt aatggaaagg ttattttcta cgcagacaaa
      181 ataaccgaca gcatgcaaac gactatcgat gaaactaacc gccggcggga gaagcagata
      241 aaatacaacg aacaacacgg cattacgccg caacagataa taaaagcccg gtcatccgtg
      301 ctaaacaagg agtacacggt taccgcagaa gctagggcat acgtagagcc cgattactat
      361 tccatggcat cggagccgtt gcccgaatat gctaccgcag acgatctgaa aacaaaaatc
      421 gaacagaccc gcaaggcaat gctgtcagct gccaaaaaac tggaattcct ggaggcggca
      481 aggctgcgtg atgagttgat aaaattagaa gatgagctgg aagagaagtc taaatgctga
      541 tgtctattgg cctgttattc tttgttctta aaacatacat atcttaaaac cgcatataaa
      601 tgaaatcaga cattcagatt gcaagagaga caccgctaaa aaaaataaag gaagtagcag
      661 aaaacatagg tatcgaccgg gatgaggttc ataactacgg cccgtacatg gcaaaagtgc
      721 ccttgcggtt gaaaaatcag aaaaaaattg accaaagcaa cctggttttg gttacagcta
      781 tcacgcccac caaggcaggt atcgggaaga ctaccgtttc cattggtctg gcactgggac
      841 tgaacaaaat tggtaaaaaa gccattgtag cgttacgtga accctcactg ggacccgtat
      901 ttggaatgaa aggaggagct gctggcggcg ggtatgccca agtgcttccc atggagaaca
      961 tcaatctaca ctttaccggt gattttcatg cggtcacatc ggctcataac actatatcgg
     1021 cacttctgga caattatatt tttcgtgttc agggtacacc tcatgagatc aaggaggtgc
     1081 tttggaaacg ggtgttggat gtgaacgacc gcagtttgcg ctatattgtt accggcctcg
     1141 gccccggaaa cggagtcccg cttgaaggtg gtttcgatat tacagccgca tcggaattga
     1201 tggccatttt gtgtctggcg gaggatgaag aggacctgcg tcgacgcatc gaaaatattt
     1261 tattgggtta tacgcgggaa gacaagccgt ttacggtaaa agacttgggt gttgcaggtg
     1321 ccataaccgt actgatgaaa gacgccattc atcccaattt ggtacaaacg actgagaata
     1381 cggcagcatt tgttcatgga ggcccctttg caaatatcgc acacggttgc aattcggtct
     1441 ctgctacaaa aatggccatg tcgtatggcg agtatgtgat taccgaagcc ggtttcggtg
     1501 ccgatctggg agcggaaaag ttttttaata ttaaatgtag aaaaagtggt ttgcaaccca
     1561 aactaacggt tattgtcgtt actgcgcagg gactgaaaat gcacggcgga actcctgaaa
     1621 ccgatatcag acagcctgac atggaaggac tgaaaaaagg gttggagaac ttgtcgaaac
     1681 acctggagaa tctggcctct tttggtcaaa ctgttgtggt ggcattcaac aaatatgctt
     1741 ttgatacaca ggatgaaata gatgctgtgg ctgagttttg tcgccaacgt ggggttcgtt
     1801 ttgccgtaaa caatgcgttt gtagaaggcg ggcagggagc cgttgagctg gctaatgcag
     1861 tagtggaagc cattgaaaaa gagccgtctc aaccgttaca gtttacttat tccgatgaag
     1921 attccatccg gacaaaactc gaaaaaattg cacttaatat ttacggtgca cgggatgtag
     1981 tctttaacga aaaggcatta aggcgattga agagaataga cgatactcca ctggcaaaat
     2041 ttccggtttg catagcaaaa acgcaattct cgttctctgc cgatccaaag gagtatggtg
     2101 tagccagagg atttcgcttt aagatcaacg atattgtaat taaccagggc tcggagttta
     2161 ttgtcgctat tgccggcgaa atgatgcgta tgcctggcct tcccgctgat ccacaagcca
     2221 aaaggattga tattgtgaac ggaaaagtgg aaggattgag ttagttgcaa tttaggattt
     2281 atggtgtgag aatatcactt ttatcgacga taatttgaaa tatcgttatt tgatacgata
     2341 ttttgatata tcattttttc tcacgatatt tgtcggatga tagcagtaat aaaaggagac
     2401 attgtttcgt ctcgaaaagt agaaaattcc gaaaagtggc tattgccgct gaaaagcttg
     2461 ttcaatgaat ggggaaaatc gcccggacag tgggaaatcg tatggggcga ttcgtttcaa
     2521 ctggaagtag atcgcccgga agaagccctg aaaaaggcgt tacgggtgaa agcattaata
     2581 aagaagatag tttctgatga aacaaataaa a
//
LOCUS       DAOSDR010000416         2597 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrimonas sp. isolate AMR_MDS_1826
            NODE_13916_length_2597_cov_4.165618, whole genome shotgun sequence.
ACCESSION   DAOSDR010000416 DAOSDR010000000
VERSION     DAOSDR010000416.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863355
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrimonas sp. (wastewater metagenome)
  ORGANISM  Petrimonas sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Dysgonomonadaceae; Petrimonas.
REFERENCE   1  (bases 1 to 2597)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2597)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:10:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,720
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 35
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2597
                     /organism="Petrimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13916_length_2597_cov_4.165618"
                     /isolate="AMR_MDS_1826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2023866"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..640
                     /locus_tag="PLI30_12705"
     CDS             <1..640
                     /locus_tag="PLI30_12705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006798529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HOI80451.1"
                     /translation="GLDHVLAMGQDINVLVLDTEVYSNTGGQSSKSTPTAAVAKFAAA
                     GKRIRKKDLGMIAATYGYVYVAQVAMGANQAHYLKALKEAEEYNGPSLIIAYSPCISH
                     GLRSGMGMAQTEQKKAVECGYWSLWRYNPKLEEEGKNPFVLDSKEPDWSKFRDFLMGE
                     VRYTQLVKSFPHEADELFEAAKENAQWRYRSYQRMANAHFGSVDAETAKAEG"
     gene            774..1205
                     /gene="aroQ"
                     /locus_tag="PLI30_12710"
     CDS             774..1205
                     /gene="aroQ"
                     /locus_tag="PLI30_12710"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019538536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II 3-dehydroquinate dehydratase"
                     /protein_id="HOI80452.1"
                     /translation="MKIQIINGPNLNLLGMREPSVYGDQSFSAFFERLKEVFPEIRLD
                     HYQSNVEGEIINKIHETGFDYDGIILNAGGYTHTSVAIRDAIKSVRTPVIEVHISNVY
                     AREEFRHQSMISAVCAGVIGGFGLNSYRLAVEALIMANKTD"
     gene            1264..>2597
                     /gene="pyk"
                     /locus_tag="PLI30_12715"
     CDS             1264..>2597
                     /gene="pyk"
                     /locus_tag="PLI30_12715"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008779098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HOI80453.1"
                     /translation="MLKHTKIVATISDKRCDVPFIQQLFAEGMDVVRLNSAHLDQEGF
                     LKIINNVRAVSDRIAILVDTKGPEIRTTVASEPIELTTGDRVKVIGNPGSVSSKEAIY
                     VSYPNIVDEMNVGDDILIDDGEIDLKVIDKDAGYLLCSVQNDGTVGSRKSVNIPGVRI
                     NLPSITERDRKFIALSAKHDVDFIAHSFVRNKEDVLEVQKILNELNSPIKIIAKIENQ
                     EGVDNIDEILRHAYGIMIARGDLGVEVAQEKIPAIQRTLIRKAIHHKKPVIVATQMLH
                     TMIEYPRPTRAEITDVANAIYYRTDAIMLSGETAYGKYPVEAVRTMTKVAFETEKTKL
                     PQNDIAVPYQPLDDNEVTSFLAKQAVKSSDRLETKAIVTDSHTGRTARVLAAFRGKSP
                     VYAITTTERLARELALSYGVWAHHQSGKGDGNTKESRRAYFVGAITKLIENK"
BASE COUNT          758 a          551 c          651 g          637 t
ORIGIN      
        1 cggcctcgac cacgtgttgg ctatggggca ggatatcaat gtgctggtgc tcgataccga
       61 agtatattcc aataccggcg gacaatcctc gaaatcaacc cctacagctg ccgttgccaa
      121 atttgccgct gcagggaaac gtatccgcaa aaaagacctg ggtatgattg cggcgactta
      181 cggttatgta tacgtggctc aggtagctat gggcgccaat caggctcatt acctcaaggc
      241 attgaaagaa gccgaggaat acaacggccc gtcgcttatt attgcttatt cgccttgtat
      301 cagccatggg ttaagaagcg gtatgggtat ggctcagacc gagcagaaaa aagccgttga
      361 atgcggttat tggagcttgt ggcgttacaa cccgaaactg gaagaagagg gcaaaaatcc
      421 gtttgtactc gacagtaagg aacccgactg gagtaagttc agggatttcc tgatgggtga
      481 agtgcgttac acgcagctgg tgaaatcttt cccgcacgaa gccgatgaac tttttgaagc
      541 ggccaaggaa aatgcccagt ggcgttaccg ctcataccaa cgtatggcca atgcccactt
      601 tggctcagtg gatgctgaaa ctgcgaaagc agaaggataa caattgcata ttcatgaaca
      661 taaaaaagcg atacgtcggt cggcgtttcg ctttttttgc cggtttgctt tgattttttt
      721 aatcgtcgga ctgcgttaaa atcgtatctt tgcaaccaca aacatataca acaatgaaaa
      781 tccaaattat caacggacct aatcttaact tgttggggat gagggaaccc agtgtttacg
      841 gagatcaatc tttctcggcc tttttcgaga ggttgaagga ggtgtttccc gaaatcaggc
      901 tggaccatta tcaatcgaat gttgaagggg aaatcatcaa taagattcat gagacggggt
      961 ttgattatga cggcatcatc ctcaatgccg gggggtatac ccacacctcg gttgctattc
     1021 gcgatgcgat taaatcggtg aggaccccgg tgatcgaagt acatatctcc aacgtatatg
     1081 cccgggaaga gttccgtcat caatccatga tctcggcagt ttgtgcaggg gtgatcggtg
     1141 ggtttggact gaactcctat cgtttagctg tggaagcctt gataatggca aacaaaacgg
     1201 attaaaaagt gtttaatggt cagatgacat ctgagatcta aaaaattctc aactaaatta
     1261 acaatgctaa aacatactaa aattgtagcc acaatttcgg ataaacgttg cgatgttcct
     1321 ttcattcagc agctttttgc agaaggaatg gatgttgtca ggttaaattc ggctcacctt
     1381 gaccaggaag gatttctgaa aattataaac aacgttcgtg cagtgtcgga cagaattgcc
     1441 attctggttg atacgaaagg cccggaaata aggacaactg tagcttccga acccatcgaa
     1501 ctcaccactg gcgaccgggt gaaggttatc ggtaatcccg ggagcgtttc atccaaggag
     1561 gctatttatg tctcttaccc gaacattgtc gatgagatga acgtgggtga cgatattctg
     1621 attgatgacg gagaaattga cctgaaggtg atcgataaag atgctggtta cctgctttgt
     1681 agtgtgcaaa atgacgggac ggtcggaagc cggaaaagcg tgaatattcc cggagtccgt
     1741 atcaacctgc cgagtataac cgaacgcgat agaaaattta ttgccctatc ggcaaagcac
     1801 gatgtcgatt ttattgcaca ttcgtttgtg cgaaacaaag aagatgtatt ggaagtccag
     1861 aaaattttga atgaactgaa cagtccaata aaaattatcg cgaaaattga gaatcaagaa
     1921 ggtgtggaca acatcgatga aatattacgc catgcctatg ggattatgat tgcgcgtggt
     1981 gatttgggag tggaggtggc tcaggagaaa attccggcta ttcagcggac acttatccgg
     2041 aaagctatcc atcataaaaa accggttatt gtcgcaacgc aaatgttgca caccatgatt
     2101 gaatatcccc gcccgacgcg tgccgaaata accgatgttg ccaatgccat ttattaccgg
     2161 accgacgcta ttatgttaag cggtgaaaca gcttacggaa aatatccggt ggaggcggtg
     2221 agaaccatga caaaagtggc ttttgaaacc gaaaaaacaa aactgccaca aaacgatatc
     2281 gccgttccat accaaccttt ggacgataac gaagttactt cgttcctcgc caaacaagcc
     2341 gttaaatcga gcgaccggtt agaaacaaag gctatcgtta ccgacagcca tacgggtaga
     2401 acagcccggg ttttggctgc tttccggggt aaaagtcctg tttatgccat taccacaacc
     2461 gagaggctgg cacgcgaact ggccctgtct tacggagtat gggcacacca tcagagtggc
     2521 aaaggagatg gaaacaccaa agaaagccgc cgggcctatt ttgtgggtgc gataacaaaa
     2581 ctgattgaga ataagat
//
LOCUS       DAOSDR010000417         2586 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrimonas sp. isolate AMR_MDS_1826
            NODE_14000_length_2586_cov_3.248123, whole genome shotgun sequence.
ACCESSION   DAOSDR010000417 DAOSDR010000000
VERSION     DAOSDR010000417.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863355
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrimonas sp. (wastewater metagenome)
  ORGANISM  Petrimonas sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Dysgonomonadaceae; Petrimonas.
REFERENCE   1  (bases 1 to 2586)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2586)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:10:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,720
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 35
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2586
                     /organism="Petrimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14000_length_2586_cov_3.248123"
                     /isolate="AMR_MDS_1826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2023866"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..450)
                     /locus_tag="PLI30_12720"
     CDS             complement(<1..450)
                     /locus_tag="PLI30_12720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010803086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HOI80454.1"
                     /translation="MKVLDFIVRSNEPLNYQNNLIKVTPADNDTLPDMFPGQFVQVLV
                     SNSGNVFLRRPISINFVDKEKNEMWLLIQRVGEGTRKICETPAGQLLNLIFPLGNSFT
                     IPKKEGKYLLVGGGVGTAPMLFLGKKLNEKGYSPEFLLGGRSQRDILQ"
     gene            complement(587..1135)
                     /locus_tag="PLI30_12725"
     CDS             complement(587..1135)
                     /locus_tag="PLI30_12725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019540848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HOI80455.1"
                     /translation="MKSVKCLFVLLFTLAFTQLNAQIDLDVPYVPTPMEVVDVMLDLA
                     KVNRSDVVYDLGCGDGRIVITAAKKYGATGIGVDLNPERIEEANAYAKKKGVEKKVKF
                     YEGNLFDFDFSKATVLTLYLLPDVNLKLMPKILKEMKPGSRVVSHAFDMGDWKPDKQV
                     SVDGRTVYLWTVPKRKDSSRIK"
     gene            complement(1251..>2586)
                     /locus_tag="PLI30_12730"
     CDS             complement(1251..>2586)
                     /locus_tag="PLI30_12730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019540849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HOI80456.1"
                     /translation="CPPAAPKPTLRLIDAIVLITGIVIGAGIFRTPSVVAANTPDGFW
                     FLSVWVLGGLVSLAGALCYSELSSAFPSAGGDYHFLRMAFGNGFSFLFAWARIAVIQT
                     GSIAILAYIAGDYMSKLLSFGEHSASFYAVLVVVLLTLVNRLGIQFGTGLQKLLSALQ
                     FTGILIIIVVGFFVEPDISSTASPGIESSPSFQSFSLALIFVLLTFGGWNEAAYVSSE
                     LKAGSRKMVFVLVAGILIITAVYLLINMAYLNVLGLPGMAASDAVGVDMMRVRLGKKG
                     VVLIGLLVIFSALTSLNTTIFTGARSNYALGKDFSLFATLGKWNERKSAPLNSLLIQG
                     VIAVLLIIFGAFSRSGFESMVDFTAPVFWFFFLCMGIGLIVLRVKMPDVPRPFKVPLF
                     PVTPLLFVVFCGYLLYSSLSYTGKGALLGAALLIIGLIFYLLFKNKIVARKD"
BASE COUNT          827 a          622 c          487 g          650 t
ORIGIN      
        1 ttgaagtata tccctttggg aacgtccccc taacaggaac tcgggagaat accctttttc
       61 attgagtttt ttccccagaa aaagcatggg agcagtgccc actccccctc ccacaagcag
      121 gtatttgccc tctttcttcg gaatggtaaa ggagttgccc agcggaaaaa tcagattcaa
      181 tagttgacct gcaggtgttt cacatatttt tcgggttcct tcacccacac gctgtatcaa
      241 aagccacatt tcattttttt ccttatctac aaagtttatg gatataggcc ttcgaagaaa
      301 cacattgcct gaattactta ctaaaacctg aacaaactgt cccggaaaca tatcgggcaa
      361 cgtatcatta tctgccggag taaccttgat caggttgttc tggtaattaa gaggttcgtt
      421 tgagcgaaca atgaagtcga gtactttcat cggtgaaaac tttattgggt acaaatttac
      481 actataattt tcaattcctg cacaggcgca caaaaataaa accaggcttg caaagggcaa
      541 acctggttct gaagcaggat tacggttttt taaaaacccc ttttctctat tttatccggc
      601 tgctatcttt tcttttggga accgtccaca aatataccgt tctgccatct actgatacct
      661 gcttatctgg cttccagtca cccatgtcaa aagcatgaga aacaaccctg cttcccggtt
      721 tcatttcttt cagtattttc ggcattaatt tcaggttgac atccggtagc agataaaggg
      781 ttagcacagt agctttactg aaatcaaaat cgaaaagatt tccttcgtaa aatttcactt
      841 tcttttctac tccttttttc ttagcataag cattggcctc ctcaatcctt tccgggttca
      901 gatccactcc tatcccggta gctccatatt ttttggcagc agttatcact atacgcccat
      961 caccgcatcc caaatcgtaa acgacatcgc tcctgttcac ttttgccaaa tcaagcataa
     1021 catctaccac ctccatcgga gtaggaacat agggaacgtc aagatctatc tgggcattta
     1081 actgtgtgaa tgccaacgtg aataacaaaa caaacaagca cttgactgat ttcatgttgt
     1141 gtaattttta attgttagaa aataagggtc ttatgtaata aatcaacggg taccgttaat
     1201 tgttcaacca atgtaagaat aaataaggta aaattaacaa tttaagactg ttaatccttc
     1261 ctggctacaa tcttgttttt gaaaagaagg taaaaaatca gcccgatgat taaaagtgcc
     1321 gcccccaata aagcaccttt gccggtataa gacagacttg aataaagtaa atatccacaa
     1381 aacacaacaa acaagagcgg ggttaccggg aacaacggaa ctttgaacgg tcgtggtacg
     1441 tcaggcatct ttacccgcag tacaatcaat ccaatgccca tacacaaaaa gaaaaaccaa
     1501 aaaacgggtg ctgtaaaatc gaccatggat tcgaatccgc tccggctaaa tgctccgaaa
     1561 attataagca aaacagcaat gacaccttga ataagaaggg aattgagcgg cgcagacttt
     1621 ctttcgttcc attttccgag agtggcaaac agactgaaat cttttcccag tgcataattg
     1681 gatcgtgcac ccgtaaaaat agttgtgttt aatgatgtta aagcagagaa tattaccagc
     1741 aagcctatta aaacaacccc tttttttcca agcctaaccc tcatcatatc caccccaacg
     1801 gcatccgacg cagccatccc aggaagtccc aacacattca aataagccat attgataaga
     1861 aggtacaccg cggttatgat caaaataccc gcaaccagca caaataccat cttcctactg
     1921 ccagccttca actccgaaga gacataagct gcctcattcc atccgccaaa agtcaataaa
     1981 acaaaaataa gagccaggct gaatgattga aaagagggcg atgattcaat acccggagag
     2041 gctgttgacg aaatatcggg ctctacaaaa aaacccacta cgatgatgat cagtatgccg
     2101 gtaaactgca atgcggacag aagcttttga aggccagttc cgaattgaat tcccagccta
     2161 tttaccaggg tgagcagaac cactacgaga acagcataaa aagaggctga atgttcgcca
     2221 aacgatagca gtttcgacat atagtcaccc gcaatataag ccagtatagc aatggagcct
     2281 gtttgaataa ccgcaatacg cgcccaggca aacaagaaag agaacccatt accaaacgcc
     2341 attcttaaaa aatggtaatc gcctccagca ctgggaaagg cagagctaag ctccgaatag
     2401 caaagcgcac cggcaagaga aaccaggcct cccagcaccc agacactcaa gaaccaaaat
     2461 ccatcgggag tgttcgctgc aaccaccgac ggtgttctga aaattccagc accgatcaca
     2521 atgcctgtaa tcagaacaat agcgtctata aggcgtaatg taggtttggg agctgcgggc
     2581 ggacat
//
LOCUS       DAOSDR010000418         2585 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrimonas sp. isolate AMR_MDS_1826
            NODE_14005_length_2585_cov_3.487352, whole genome shotgun sequence.
ACCESSION   DAOSDR010000418 DAOSDR010000000
VERSION     DAOSDR010000418.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863355
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrimonas sp. (wastewater metagenome)
  ORGANISM  Petrimonas sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Dysgonomonadaceae; Petrimonas.
REFERENCE   1  (bases 1 to 2585)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2585)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:10:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,720
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 35
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2585
                     /organism="Petrimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14005_length_2585_cov_3.487352"
                     /isolate="AMR_MDS_1826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2023866"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..361)
                     /locus_tag="PLI30_12735"
     CDS             complement(<1..361)
                     /locus_tag="PLI30_12735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019539756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase family
                     protein"
                     /protein_id="HOI80457.1"
                     /translation="MNQKNVIITLIFLTFVFSATSQEIIAHRGYWKTEGSAQNSVAAL
                     MNADALDVYGSEVDIWLSSDGVPVVNHDADVLLDGKKLRVQETSAAVLRKVKLANGEP
                     LPTVEEYLTAFEKCGNTR"
     gene            complement(381..1856)
                     /locus_tag="PLI30_12740"
     CDS             complement(381..1856)
                     /locus_tag="PLI30_12740"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HOI80458.1"
                     /translation="MIDNYSLPRSTPEAEGLSSEAVIQFVDAAESVRDSSIELHSFMI
                     VRHGKVLAEGWWSPYRHDLKHTMYSVSKSFTSTAIGLAVAEGKLKLTDKVVSFFPESL
                     PDTVSPYLAKLDVDDLLKMSVGQAREPQEMRFSDDWIKTFLAAPVQYEPGTVFLYNSA
                     GSFMLSAILQKATGEKLIDYLKPRLFEPLGIKDADWEVNPQGINVGGWGLRVKTEDMA
                     KLGLLYLQKGKWRDRRILTEEWVNAATSEQIKTVTGEEDPANDWAQGYGYQFWQTRNN
                     AFRGDGAFGQFIIVLPEKDAVIVLTANAQDMQKEINLVWKHIFPAFRESALAEDGDAL
                     DRLKSRLSALRLPVHTTRTSSPVREIISGKTMAFNKNPLKISELTLRFEGDVCRMTLR
                     QDTATYEFRFSSTSWEEGETLRHGPSLTAGSKAGLTGLPPFKVAGNYVWKDEKTLELV
                     LRYIESPNDERFVFSFEKPAVSVEYSASYDFYQQKLRWTEK"
     gene            complement(2090..>2585)
                     /locus_tag="PLI30_12745"
     CDS             complement(2090..>2585)
                     /locus_tag="PLI30_12745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI80459.1"
                     /translation="RGDNECLRAVGILSAAPRYSDEGWLSSAFEVMERFPDPPFDSLG
                     IPTWHYGHELPTPFATKIAKYFENSIREEGLLAIAKGGVVFTPGSAGTLQEVFQDLAQ
                     NHYESYGYASPMIFLDKHFWTTERPVYPVIGEMAERGDLHHLNLGLYDNNEEVIAHLK
                     KFSE"
BASE COUNT          601 a          663 c          643 g          678 t
ORIGIN      
        1 gccgggtgtt cccgcatttt tcaaacgcag ttaaatattc ttctaccgta ggcaacggtt
       61 caccgtttgc taatttcact ttccggagga cagcggcaga agtttcctgc acccgtaatt
      121 tcttcccgtc gagcagcaca tcggcgtcgt gatttaccac aggcacgccg tccgacgata
      181 gccagatatc tacttcagat ccgtaaacgt caagcgcatc ggcgttcatt aaggcagcaa
      241 cggagttttg tgcggaaccc tcagttttcc agtatccgcg atgcgcaatg atttcctgtg
      301 acgttgcgga aaaaacaaac gtcagaaaga tcagggtaat gattacattt ttttgattca
      361 tgattgtcgg atttatattg ttatttttca gtccaccgta gtttctgttg ataaaaatcg
      421 taactagctg aatactctac cgaaacggcc ggtttttcga aagaaaagac gaaccgctcg
      481 tcgttggggc tctctatgta acggagcacc aactccagtg ttttttcatc tttccagaca
      541 tagtttcccg ccaccttaaa gggtggtaag cccgttaaac cggctttgga gccggcggtg
      601 agcgaaggcc cgtggcgcaa cgtttcgcct tcctcccacg aagtggagct gaaccggaat
      661 tcataggttg ccgtgtcttg ccttagggtc atccggcaaa cgtcgccttc gaatcgcaat
      721 gttaattccg atattttcag tggatttttg ttgaaagcca ttgtttttcc ggaaataatt
      781 tcccggacag gagaggaggt tctcgtggtg tggacaggta acctcaacgc cgacaaacgg
      841 gatttcaacc tgtccagcgc gtcaccgtcc tcagccaacg cagattcccg gaaagcagga
      901 aaaatatgct tccagaccag gttgatctcc ttttgcatat cctgagcgtt agccgtaagg
      961 acaataaccg catctttctc cggcaggaca atgatgaact gcccgaaagc cccgtcgccg
     1021 cggaacgcgt tatttcgcgt ttgccagaac tgataaccgt aaccttgtgc ccagtcattc
     1081 gccgggtctt cttctcccgt tacggtcttg atctgctcgg aagtagccgc attcacccat
     1141 tcttccgtca ggatccggcg atctctccat ttaccttttt gcaagtagag cagccccagt
     1201 ttcgccatat cttccgtctt aacgcgcagc ccccatccgc cgacgtttat tccctgcggg
     1261 ttcacttccc aatcggcatc tttaatgccc aaaggttcaa acaggcgggg cttgaggtaa
     1321 tcgatgagtt tttcacctgt cgctttttgc aggatggcag acagcatgaa cgatcctgcc
     1381 gagttataaa ggaaaaccgt tcccggctcg tattggacag gagcggccaa aaacgttttt
     1441 atccaatcgt cgctaaagcg catctcctga ggttcccggg cctgtcccac ggacattttc
     1501 agcaggtcgt ccacatcgag cttcgcgaga taaggagaga ccgtgtcggg caatgattcg
     1561 ggaaaaaacg aaaccacctt gtcggtcagc ttcagcttcc cttccgccac agccagtccg
     1621 atggccgtag aagtaaagct tttggacacg gaatacatcg tgtgcttgag gtcgtgccgg
     1681 tagggtgacc accagccttc ggccaggact ttcccgtgcc gtacaatcat gaaactgtgg
     1741 agctcgatgc tgctgtcgcg aacgctctcg gcggcgtcga cgaattggat gaccgcctcg
     1801 gacgataagc cctctgcttc aggtgtactg cggggcaacg agtaattgtc gatcattttt
     1861 tgttccgttt tttgaacaca tccactcaac atgaggatca atccaaatag gggaaaaagg
     1921 agcgagtttt tcatttactg cctgtttaat cgatgaattt ggttcaaagg tacggaaatt
     1981 tataattttt cagaaacagg cttgaatttt ttaaagaata tggttttctt ttcgcattat
     2041 tttatgatcg agatgttaaa atacggagcc agaaagtgac ggtaccggtt tattccgaaa
     2101 actttttcaa gtgggcgata acctcttcgt tgttatcgta gaggcccagg ttgaggtgat
     2161 gcaaatcgcc gcgttccgcc atttctccga tgacggggta gacggggcgt tcggttgtcc
     2221 agaagtgctt atccaaaaaa atcatcgggc tggcgtaacc gtatgactcg taatggtttt
     2281 gtgccaggtc ctgaaaaacc tcctgcaggg tccccgcact tccgggggtg aaaaccacgc
     2341 ctcctttggc gatggcgagc aacccttctt cgcggatact gttctcgaaa tatttggcaa
     2401 ttttcgtggc aaatggggtg gggagctcgt gcccgtaatg ccatgtggga attcccagac
     2461 tatcgaaagg cgggtcgggg aaacgttcca ttacctcgaa tgccgaagaa agccaaccct
     2521 cgtcggaata gcggggggcc gcactcaaga tgccgacggc ccgaagacat tcgttgtcgc
     2581 ccctt
//
LOCUS       DAOSDR010000419         2555 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrimonas sp. isolate AMR_MDS_1826
            NODE_14193_length_2555_cov_2.966400, whole genome shotgun sequence.
ACCESSION   DAOSDR010000419 DAOSDR010000000
VERSION     DAOSDR010000419.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863355
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrimonas sp. (wastewater metagenome)
  ORGANISM  Petrimonas sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Dysgonomonadaceae; Petrimonas.
REFERENCE   1  (bases 1 to 2555)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2555)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:10:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,720
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 35
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2555
                     /organism="Petrimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14193_length_2555_cov_2.966400"
                     /isolate="AMR_MDS_1826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2023866"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..331
                     /locus_tag="PLI30_12750"
     CDS             <1..331
                     /locus_tag="PLI30_12750"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019540190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HOI80460.1"
                     /translation="ADFNDPKLVKVVELEMACRQQQVNENLFTGIDLVPTHAITVTIP
                     ALLRAKYMFCMVPAENKAKAVYHTLNDDISEDCPATVLRTKKGTVLYLDKESASFLET
                     TNNTADK"
     gene            369..1367
                     /locus_tag="PLI30_12755"
     CDS             369..1367
                     /locus_tag="PLI30_12755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007763667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOI80461.1"
                     /translation="MRIRVILLTILAFVLTQAQAQIKIGIIGLDTSHAIAFTKFLNGE
                     DKKEEFKEFKIVAAYPYGSKTIKSSFDRIPGYIEQVKGLGVQIVPSIAELLKKVDYVM
                     LETNDGNLHLEQAYEVFKAGKPMFIDKPLGATLAQSIAIYQLAREYNVPIFSSSALRY
                     VPQNQKLRKGEFGKVLGADCYSPASREKTHPDFGWYGIHGVETLFTIMGTGCVSVNRM
                     SAEGTDVVAGLWSDGRIGTFRGLRTGKSNYGGTAFTDKSIEPVGPYQGYEVLLTEILN
                     FFKTRVVPVSEEETLEIFTFMEASNESKRNDGKIILMEDTYRKGLAEARKLLSDLK"
     gene            1375..>2555
                     /locus_tag="PLI30_12760"
     CDS             1375..>2555
                     /locus_tag="PLI30_12760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019540188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative oxidoreductase C-terminal
                     domain-containing protein"
                     /protein_id="HOI80462.1"
                     /translation="MKSFLLTGIIGLMFLSCMQQSREKTSNSTVFTGKAGEVKLIVLA
                     PGHFHANLLQKSTIDQVNDSVYVYAMSPDDAGLNQYLSAIESFNNRAENPTSWQEVIY
                     TGDDFVDKMVTDENGNVVVLAGNNKNKTEYILRAVDAGLNVLSDKPMAINRNDFLLLE
                     EAYKNAGSKNVQLYDMMTERYDMLNIIEKELINNPDFFGELKVGTPEDPAVYMESVHH
                     FYKEVSGSPLIRPAWYYDVEQQGEGIADVTTHLIDLLFWKCFPDQPIDYTRDIGSISS
                     THWPTNISRQQFMQSTGESSFPDYLHKYLDNSTLKVNANGTVNFDVKNHNVSLQVMWN
                     YQAPEGGGDTFISTLKGTKAILRTVQNKEQNFIKQLYVYKAEGVSEKEFAENIQQVVT
                     K"
BASE COUNT          799 a          481 c          587 g          688 t
ORIGIN      
        1 tgccgatttt aatgatccaa aactggtgaa ggtggtagag ttggagatgg cttgcaggca
       61 acaacaggta aatgaaaacc tttttacagg aatagacctg gtacccaccc atgctatcac
      121 cgtaaccatt ccggcattgc tcagagcgaa atatatgttt tgtatggtgc ctgccgaaaa
      181 caaagcaaag gctgtgtatc atactcttaa cgatgatatt agtgaggatt gtcctgctac
      241 ggtcttaaga acaaaaaagg gaactgtatt atatctcgac aaagagagtg cttcgttcct
      301 ggagacaact aacaacacag ccgataaatg aatttatgtt tttcacaaca tcaaaaaata
      361 gaaatattat gagaattaga gttatattgt taacaattct agcctttgtt ttaactcagg
      421 ctcaagcaca gattaagatt ggtatcattg gcctggatac ctctcatgca atcgccttca
      481 ccaagttttt gaatggagaa gacaaaaaag aggagttcaa agagtttaaa attgtcgcag
      541 cctatcccta cgggtcgaaa accattaagt caagttttga ccgtattccc ggttacattg
      601 aacaggtaaa aggtttggga gtgcaaattg ttccttccat tgctgaactt ctgaagaaag
      661 tggattacgt aatgttggaa acaaatgatg ggaacctcca tctggagcag gcatatgagg
      721 ttttcaaagc cggaaagccc atgtttattg acaaaccact tggtgctaca cttgcgcaat
      781 cgatagcgat ctatcagctg gccagagaat acaatgtccc tattttctct tcctcagcgt
      841 tgcggtatgt tccacaaaat cagaaattac gcaaaggaga atttggaaaa gttttgggag
      901 ctgattgtta ctcccccgca agtcgcgaaa aaacacatcc cgattttggg tggtacggca
      961 tccatggggt agaaaccctt ttcaccatca tgggaaccgg atgcgtttcg gttaaccgca
     1021 tgtcggctga aggtactgat gtagtagctg gattatggag tgatggaaga ataggtacgt
     1081 tcagaggcct tcgtaccggg aaaagcaatt acggaggaac agccttcacg gacaaaagca
     1141 tagagccggt tggcccttac cagggatacg aagtgctgct cacagagata cttaattttt
     1201 ttaagactcg tgttgtccct gtttccgaag aagaaacact ggaaatcttc acttttatgg
     1261 aagcttccaa tgaaagcaag cgcaacgatg gaaaaattat cctgatggaa gatacttatc
     1321 ggaaaggttt ggcagaagcc aggaaattac tttcggattt aaagtaaaag tgaaatgaaa
     1381 agttttttgt tgaccggtat cattggtctg atgtttctat cttgtatgca gcaaagtaga
     1441 gaaaagacaa gtaattctac ggtatttacc ggtaaagcag gcgaagtgaa attgatcgtt
     1501 cttgcgccgg gacattttca tgccaatctg cttcaaaaaa gtaccatcga ccaagtgaat
     1561 gattcagttt acgtttacgc catgtctcct gatgatgccg gattaaacca atatctttca
     1621 gctattgagt cattcaacaa cagggcagag aatcctacga gctggcaaga ggttatatac
     1681 acgggtgacg actttgtgga caagatggtg acagatgaaa atggtaatgt tgtggtactt
     1741 gccggtaaca acaaaaacaa gaccgaatat attctccgcg ctgtggatgc cggattgaat
     1801 gtgttgtccg acaagccgat ggctattaac aggaatgatt ttcttttgct ggaagaagcg
     1861 tataagaatg cagggtcaaa aaatgttcaa ctgtatgata tgatgacgga acgctacgat
     1921 atgctgaaca tcatcgaaaa agagttgata aacaatcccg atttttttgg agaacttaag
     1981 gtgggaacgc cggaagatcc tgctgtctat atggaaagtg tgcaccattt ctataaagag
     2041 gtttcgggat caccgttgat tcgtccggca tggtactacg acgtggaaca acagggagag
     2101 ggtattgcag atgtcactac tcacttaatt gacttgctgt tctggaaatg ttttccggat
     2161 cagcctatcg attatacacg ggacatcgga agtatctcat ctactcactg gccgactaat
     2221 atctctcggc agcagttcat gcagtctacc ggagaatcat catttcctga ttatcttcat
     2281 aaataccttg ataattcaac gctgaaagtc aatgccaacg gaactgtaaa cttcgatgtg
     2341 aaaaaccaca acgtaagttt acaagttatg tggaactatc aggcacctga gggtggaggt
     2401 gacacattca tctccacgtt gaaaggcaca aaagctattt tgagaactgt tcagaataag
     2461 gaacaaaact ttatcaaaca gttgtatgtg tataaagctg aaggtgttag tgagaaggaa
     2521 tttgcagaaa atattcaaca ggtagtcacg aaaat
//
LOCUS       DAOSDR010000420         2549 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrimonas sp. isolate AMR_MDS_1826
            NODE_14234_length_2549_cov_3.481155, whole genome shotgun sequence.
ACCESSION   DAOSDR010000420 DAOSDR010000000
VERSION     DAOSDR010000420.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863355
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrimonas sp. (wastewater metagenome)
  ORGANISM  Petrimonas sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Dysgonomonadaceae; Petrimonas.
REFERENCE   1  (bases 1 to 2549)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2549)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:10:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,720
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 35
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2549
                     /organism="Petrimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14234_length_2549_cov_3.481155"
                     /isolate="AMR_MDS_1826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2023866"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1077
                     /locus_tag="PLI30_12765"
     CDS             <1..1077
                     /locus_tag="PLI30_12765"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018337398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003985 - acetyl-CoA C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HOI80463.1"
                     /translation="ESSKIDSVIVGNVLSAGQGQGVARQCAMYAGIPAEVPAYSVNMV
                     CGSGMKAVMNAVSEIRSGTASVIIAGGVENMSDAGFILPSSVRNGIKMGSIQAVDHMV
                     NDGLTDVFNNYHMGITAENVAEKFGISREAQDAFAYHSQQKAIAAVDNDKFKEEIVPV
                     EIVTRKETTVFDKDEFPNRTSTPEKLAALRPAFKKEGTVTAGNASGINDGASFVMLAG
                     EEAVAQYGLKPLAEIIAAGESGVAPEVMGLGPVPAINKALHRAGMKLSEMEIIELNEA
                     FAAQSIGVIERLCDKHGVSREWINERCNIHGGAIALGHPIGASGNRILVSLVHALKMY
                     DKIYGLASLCIGGGMGTAIIIKKY"
     gene            1280..2059
                     /locus_tag="PLI30_12770"
     CDS             1280..2059
                     /locus_tag="PLI30_12770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013613329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HOI80464.1"
                     /translation="MEEKKIIVQRDNHLATVIINEPKTLNAINGQMLQELDTVFDKLA
                     NDKEIRVIILTGAGRAFVAGANIQEMSGMNYEEAKAFGKKGSSVFRKIEACPQPVIAA
                     VNGFALGGGCELALACDIRIASEKATFGQPEVGLGITPGFSGTLRLARLVGLAKAKEL
                     IFTGKAVTAEEASAIGLVNRVVAPEALMEEVRSLASTIAGQAPLAVQKSKVAINNGWD
                     LPVSEGIELETELFAQCFETEDQKNGMSAFLKKQKTEFRGK"
     gene            2097..>2549
                     /locus_tag="PLI30_12775"
     CDS             2097..>2549
                     /locus_tag="PLI30_12775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013613330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HOI80465.1"
                     /translation="MKIGVIGTGTMGNGIAQAFAQGGYPVVMKDLSDELLQKAVENID
                     NSLSRLVAKEKLNEADKQQILARIATTLSYEDFNDCGLIVEAIAENIELKKKVFRELD
                     ETCPPGTILATNSSSLSITEIAACTRRPEKVIGMHFFNPVPAMKLVEII"
BASE COUNT          751 a          564 c          679 g          555 t
ORIGIN      
        1 gaaagttcaa aaatcgattc cgtcattgtc ggaaacgtct tatctgccgg acaaggacag
       61 ggtgtggccc gtcagtgtgc catgtacgca ggcattcccg ccgaagtccc agcctatagt
      121 gtcaacatgg tgtgcggtag cggaatgaaa gcggtgatga atgccgtctc ggaaatccga
      181 agtggcacag catcggtaat cattgcggga ggagttgaaa atatgtcgga tgccggattt
      241 atcctgcctt catcggtgcg caacggcatt aaaatgggca gtatccaggc tgtcgatcac
      301 atggtgaacg atgggcttac cgatgttttc aacaattatc acatgggaat tactgcagaa
      361 aatgttgctg aaaaattcgg tatttcccgt gaggcacagg atgcctttgc ctaccattca
      421 cagcagaaag ccattgccgc cgtggataat gataagttta aagaggagat tgtccctgtg
      481 gagattgtta cgcgaaaaga aactaccgtg ttcgacaaag atgaattccc gaaccggaca
      541 agcacacccg aaaagctggc ggcactgcgg ccggctttta aaaaagaggg aacggtaacg
      601 gccggaaacg catcgggaat taatgacggg gcatctttcg taatgctggc cggtgaagaa
      661 gccgtagccc agtacggatt gaaacctctg gccgagatca tagccgccgg cgaaagcggc
      721 gtcgccccag aagtaatggg cctggggcct gtccctgcca tcaacaaggc attacaccgt
      781 gcaggcatga aactttccga gatggaaatt attgagctga atgaagcttt cgcagcccaa
      841 tccatcggtg taatagagag gttgtgcgat aagcacggcg tctcacgcga gtggataaac
      901 gaacgatgca atatccacgg tggtgcgatt gccttgggac accctatcgg cgcgtcgggc
      961 aaccgcatcc tggtttcgct ggttcacgca ctgaaaatgt atgacaaaat atacggtctg
     1021 gcatcgctct gcataggcgg aggaatggga acggcgatca taattaagaa gtattgagaa
     1081 actgtttttt catgtcctgt tttatgccta tttttggatg gttttggacg cgaaaaagag
     1141 caaaaacagc atgaagaaag tataaataaa tagaacattt tcgttaagct aatgagaaaa
     1201 gccaaacttg tttggacttt gccatagcga gaaaatacct aataaaaatg aaatataaaa
     1261 ttcaaaacag tttctaagta tggaagaaaa gaaaataatt gttcaacggg ataaccacct
     1321 ggctacggta atcattaacg aaccgaaaac attaaacgcc atcaacggac agatgctgca
     1381 ggaactggac accgtttttg acaagctggc gaacgacaag gaaatccgcg tgattattct
     1441 cactggagcc ggccgtgcgt ttgttgcagg agccaacatt caggaaatgt ccggcatgaa
     1501 ttacgaagaa gcaaaggcct ttgggaaaaa gggctcatcg gttttcagga agatagaggc
     1561 ttgtcctcaa cccgtcattg ccgctgtaaa cggctttgcc ctaggtggag gttgtgaact
     1621 agcactggcg tgtgatatcc gcattgcatc ggaaaaagct acgttcgggc agcctgaggt
     1681 tgggctgggc atcacacccg gattttcggg aacgctgcgt ctagcaaggc ttgtggggtt
     1741 ggcaaaagcc aaggaactta tctttaccgg aaaggcagta accgcagaag aggcatcggc
     1801 gatcgggttg gtaaaccggg ttgtggcacc ggaagcactg atggaagaag tccgttccct
     1861 agcctctacc atagccggac aagcaccatt ggccgtccaa aaatcgaaag tggctatcaa
     1921 caacggctgg gatcttcccg tttccgaagg gatcgagctt gaaacggagc tctttgccca
     1981 atgctttgaa accgaagacc agaaaaacgg gatgagcgcg ttcttaaaga aacagaaaac
     2041 ggaatttaga gggaaataag cttaaacgag aatgtaaatt agctacatac gaacacatga
     2101 aaataggagt tataggtacc ggaacgatgg gcaacggaat tgcacaggcg tttgcacagg
     2161 gaggatatcc cgtggtcatg aaagacctgt cggacgaact gctgcagaaa gcagtggaaa
     2221 acatcgacaa cagtttgtcg cggctggtag cgaaagagaa actgaacgaa gctgacaaac
     2281 agcagattct cgctcgcatc gccaccacat tgtcgtacga agactttaac gattgcggcc
     2341 tgatcgtaga ggccattgcc gaaaatatag aactgaaaaa gaaggtattc cgggaattgg
     2401 atgaaacctg tccgcccggc accatcctgg cgaccaactc atcatcgctt tccatcaccg
     2461 agattgctgc atgtaccaga cgcccagaaa aagtgatcgg catgcacttc ttcaatcccg
     2521 taccggctat gaagctcgtt gaaattatt
//
LOCUS       DAOSDR010000421         2546 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrimonas sp. isolate AMR_MDS_1826
            NODE_14259_length_2546_cov_3.501807, whole genome shotgun sequence.
ACCESSION   DAOSDR010000421 DAOSDR010000000
VERSION     DAOSDR010000421.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863355
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrimonas sp. (wastewater metagenome)
  ORGANISM  Petrimonas sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Dysgonomonadaceae; Petrimonas.
REFERENCE   1  (bases 1 to 2546)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2546)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:10:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,720
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 35
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2546
                     /organism="Petrimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14259_length_2546_cov_3.501807"
                     /isolate="AMR_MDS_1826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2023866"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1011)
                     /locus_tag="PLI30_12780"
     CDS             complement(<1..1011)
                     /locus_tag="PLI30_12780"
                     /inference="COORDINATES: protein motif:HMM:NF024240.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOI80466.1"
                     /translation="MRLLLTNIILAFFIVTSFGQKTKSYDVVVYGGTSAGIASAIQSS
                     RMGKSVVLVEPGKRLGGLTTGGLGQTDIGNKHVVGGISREFYQNIGKYYQIPENWKWQ
                     ERSDYVDEGQTRTEKGEDAMWTFEPSAALKVYHQMLSHEKVDIVYGERLNRESGVIKE
                     GGKIISVEMENGTRYFGKMFIDATYEGDLMAAAKVSYSVGRESNNEYGETLNGIQANR
                     INRTLKWTLSRNAHNHNFIDAVDPYVVKGDPSSGLLLYIVEGGKPGIDGHGDKGIQAY
                     CFRMTLTDHPENRIPFKKPDNYKEINYELLFRNYEAAIGPIEEMYTYGDKLVPWINSP
                     MPN"
     gene            complement(1571..2494)
                     /locus_tag="PLI30_12785"
     CDS             complement(1571..2494)
                     /locus_tag="PLI30_12785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008779405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HOI80467.1"
                     /translation="MAIDRRKFIKSSLAGAAVAGLGKSLSAFAGETPAGTPSAFPIKA
                     ELRISFQEGTAPGANLNEKFDFMEEHGVVGFEPHGRGMKGRMQEFKDALKGRNIKVSA
                     VCAGFEGFILSTDPAIRKKCRDTMEELIILGGELGSTGVIIVPAFNSQVPVMPHTQET
                     RDFLCEQFNEMGNTAQKYGTTVILEPLNRKEAFYLRQVADAASICRDINNPGVTCLGD
                     FWHMTWEETCDMAAFVSAGKYLQHVHMASRKRRSMPGEDGEADNYVNGFRGLKMIGYD
                     KYVSFECGCQGDRKVVVPAALDLLRKQWKEA"
BASE COUNT          619 a          601 c          532 g          794 t
ORIGIN      
        1 gttcggcatg ggtgaattta tccagggaac cagtttatcg ccgtaagtat acatctcttc
       61 aatcggaccg atggccgctt cgtaatttct gaaaaggagc tcataattta tctccttgta
      121 gttatccggt tttttgaaag gaatcctgtt ctcggggtga tccgtcagcg tcattcggaa
      181 acagtatgcc tggatacctt tgtctccgtg tccatcaatc cccggttttc ctccttcaac
      241 gatgtagagt aacaaaccac tgcttgggtc gcctttaacc acgtaaggat ctactgcatc
      301 gataaaatta tggttatggg cgtttctgga aagcgtccat tttaaagtcc tgtttattct
      361 gtttgcctga ataccgttca acgtttctcc atattcgtta tttgattctc ggccaacgct
      421 gtacgatact ttggctgctg ccattaagtc gccctcatag gttgcgtcga taaacatttt
      481 tccgaaatat ctggtgccat tttccatttc aacggagata attttaccgc cttcttttat
      541 tacaccgctt tctctgttta atctttctcc gtacacaata tcgactttct catgagagag
      601 catttgatga tatactttca aggctgcaga aggttcaaat gtccacatgg catcttcacc
      661 tttttcggtt cttgtttgcc cttcgtctac ataatcactt ctttcttgcc atttccagtt
      721 ttcaggaatt tggtagtact tgccgatatt ttggtaaaac tctctggaga ttccgcccac
      781 cacgtgcttg tttccgatat ctgtttgacc cagtcctcct gtagtcagcc ctccgagtct
      841 tttacccggt tcgacaagaa cgaccgattt acccatcctg gaagattgaa tagcggaggc
      901 tatgcctgcg gatgtgccgc cataaacaac aacatcgtaa ctttttgttt tttggccaaa
      961 agaggttaca ataaaaaaag cgagaataat gtttgtcaat aataatctca taatttattt
     1021 ttttgaggtt attcccatcc gggatcctga gttagtctag gacttctctg tttttcaatt
     1081 ccccaatata attttcagtg aaatgacgga gcgttatgtg atcggctatg tcgttttgcg
     1141 agcatagttt ttcgcaggat ttataatcca aatatatata aatttgtaga tatagtcaaa
     1201 gaaaaataaa atttactttt gcggtgttgt tgaaaaataa atggctttaa aggactatta
     1261 ttattcaaga aatgacaatt gtgaagatac accatgcatc atctctgaaa actccaaact
     1321 ttgcggattg ctggattagg ttgaaatatt taagtcatcc gaattaattc gttttactaa
     1381 tagatatcaa gtaaggagat cctttactgt tcatttgtga atgcatagag tccaagcaac
     1441 tatttttgaa aggacactcc cgaaaatctc atccgataat gctttaaaga ataccctaaa
     1501 tttgaaaatt aacagttgct tggaactctt gtatgattta atcaaatgct tggatgacgt
     1561 ggtaaattaa ctaagcctct ttccattgtt ttctcaacaa gtcgagcgca gccgggacaa
     1621 ctacctttcg gtcgccctgg cagccgcatt caaagctgac gtacttatcg taaccgatca
     1681 ttttcaatcc gcggaaaccg ttcacgtagt tgtcggcttc tccgtcttct cccggcatgc
     1741 tgcggcgctt acggctggcc atatgcacgt gttgcaggta ttttcccgct gaaacgaaag
     1801 ccgccatgtc acacgtttct tcccacgtca tgtgccagaa gtctcccaga caggtaactc
     1861 cggggttgtt gatgtcgcgg catatggatg cagcatccgc cacctggcgg aggtaaaagg
     1921 cttcctttcg gttcagaggc tccaggataa ccgttgtgcc atacttttga gccgtgttgc
     1981 ccatttcgtt gaactgctca cacaggaaat cgcgcgtttc ttgtgtatgt ggcatcaccg
     2041 gcacctgcga attgaacgcc ggaacgatga tcactcccgt tgagccaagt tcaccgccca
     2101 ggatgatcag ttcttccatc gtgtcacggc atttcttgcg gatagcggga tcggtagaaa
     2161 gaatgaatcc ttcgaagccg gcgcaaacgg cgctaacttt gatgttacgc ccttttaacg
     2221 catccttgaa ctcttgcatc ctgcctttca tccccctgcc gtggggctcg aaccccacca
     2281 cgccgtgttc ttccataaag tcgaactttt cgttcaggtt tgcaccggga gctgtccctt
     2341 cctgaaacga tattctgagc tctgccttga tcggaaaggc ggaaggagta ccggccgggg
     2401 tttcacccgc gaaagcgctc agggactttc caaggccggc tacagcggca ccggcaagtg
     2461 atgatttgat gaattttctt ctgtctatcg ccatgattat aacgtattta ttcttttatt
     2521 tggcacttcc cggcacaggg acagta
//
LOCUS       DAOSDR010000422         2543 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrimonas sp. isolate AMR_MDS_1826
            NODE_14278_length_2543_cov_3.944936, whole genome shotgun sequence.
ACCESSION   DAOSDR010000422 DAOSDR010000000
VERSION     DAOSDR010000422.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863355
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrimonas sp. (wastewater metagenome)
  ORGANISM  Petrimonas sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Dysgonomonadaceae; Petrimonas.
REFERENCE   1  (bases 1 to 2543)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2543)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:10:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,720
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 35
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2543
                     /organism="Petrimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14278_length_2543_cov_3.944936"
                     /isolate="AMR_MDS_1826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2023866"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..731)
                     /locus_tag="PLI30_12790"
     CDS             complement(<1..731)
                     /locus_tag="PLI30_12790"
                     /inference="COORDINATES: protein motif:HMM:NF027659.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4968 domain-containing protein"
                     /protein_id="HOI80468.1"
                     /translation="MKINLRLLFFFAGLLCSFTGMNHPLNAKEMPDRFDILLQNRHVL
                     RIQVCRNEIFRIRVSSTGEFQETIMERYGIIKTDWEPTGATHIKKNGKHVISSLSYRL
                     TVDPLAGEITVTNNQGGTVVEKISLFKPADALTHELATSLDLLFRESRKDEVIIGSEN
                     SGRAVQREVQTGLLEDVGDPTKAGVIRIALKQGERFYGGGSTSRTNIQHRGTALRMWA
                     TYKKMEAPVPFITSTDGWGIFNNTTV"
     gene            complement(779..>2543)
                     /locus_tag="PLI30_12795"
     CDS             complement(779..>2543)
                     /locus_tag="PLI30_12795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI80469.1"
                     /translation="TEWLVGNPHRINLARIAFSLLGAEGKEVAMTDLQNCLQEVDLWS
                     GIISSYFEIDDIPVQVYTACHPDRDLIAVTVESPLIDLKRLAVKITFPGASAHNSRKG
                     NYIGIWDEPDRHATTYTGTKQKVIFTRELDQEKYYSALSWDTPCIFSEAEDNPHTYRL
                     TPEGNKMAFTCEFSPKQIQEKPVTAKEGLSASRKAWKQFWESGAAVDLSESEDERWFE
                     LERRIVLSRYLMKANESGSLPPQESGLVNNNGWYGRFHFEMIWWHLAHWALWDNQELA
                     DKSLGIYQDFLETARLRAKSEGFQGTKWPKCTAYNNVEWPGVAHAFLIWQQPHPIYFA
                     EMEYRQKPNRETLEKWREVVFSTAEFLASFAYYHPEKDEYVLGPPIVPVSENTPYAET
                     FNPTFELGYWRYGLKVAKEWYNRLNIDVPTKIDSVYHQLSPYPVEDSLYVLYEGIPDM
                     WTKYTFEHPAIIGIYGMLPGDDVDKVILEKTFDKILSTWNFKRIWGWDFPMLAMTAAR
                     LGRPELAVDLLLHDNYIFDEHGLAYGEGSPYPYFPSNGGLLTAIAMMAEGWDDSGDAT
                     APGFPKNGSWKIKYEQFHKIP"
BASE COUNT          622 a          628 c          573 g          720 t
ORIGIN      
        1 accgtagtat tgttaaagat accccaccca tcggtactgg tgataaaggg tacgggtgcc
       61 tccattttct tgtaggtggc ccacatgcgt aaagccgttc cccgatgttg tatattggtt
      121 cggctggtgc tcccgccacc gtaaaaccgt tccccttgtt tgagagcgat tcgaatcacc
      181 ccggctttgg tagggtcacc tacatcctcc agcagtccag tctgaacttc tctttgtact
      241 gctctgcctg aattctccga gccaataatc acctcgtctt tgcgggattc tctaaataag
      301 agatccaagg aagtagcaag ctcatgggtt agggcatctg cgggcttaaa aagtgaaatt
      361 ttttccacca cagtacctcc ctggttgttg gtgacggtga tctcacctgc aagaggatca
      421 acagtgaggc gataggaaag tgaggaaata acatgctttc cattcttttt tatgtgggta
      481 gcacctgttg gttcccaatc ggttttgata attccgtatc tctccatgat ggtttcctga
      541 aattcacctg tggaggaaac ccttattcga aagatctcgt ttcggcacac ctggatgcgc
      601 aaaacatgtc ggttttgaag caggatatca aacctgtcgg gcatctcttt ggcattcagt
      661 ggatgattca tgccggtaaa agagcatagt aatccggcaa aaaagaataa taaacgtaag
      721 ttaatcttca tataatttca gtgataattt atgtatacat gatcctcttt ccctcaagtt
      781 aagggatttt gtggaactgt tcatatttga ttttccaact accattttta gggaatcccg
      841 gggcagtagc gtctccggag tcatcccatc cttcagccat catggcaatc gctgtcagta
      901 accctccgtt tgacgggaaa tagggatatg gtgatccttc gccataggcc aatccgtgtt
      961 catcaaagat ataattatcg tgcaacagca ggtcaaccgc cagctccggc cggcctaacc
     1021 gggctgctgt cattgccagc atcggaaaat cccatcccca gatcctttta aaattccagg
     1081 tggaaagaat cttgtcgaag gtcttttcca atattacttt atccacatca tcgccgggta
     1141 acatgccata aataccaatg atggcaggat gctcaaaagt atatttggtc cacatatcgg
     1201 gtatcccctc atacaggaca taaaggctat cttccaccgg atagggtgat aactggtgat
     1261 aaacactgtc tatttttgtc ggaacatcga tattgagtct gttgtaccat tctttagcta
     1321 ccttcagtcc gtaacgccag taacccagtt caaatgtggg attgaacgtt tcggcataag
     1381 gtgtgttttc tgaaacggga acaatgggag gccccagaac atattcatct ttttccggat
     1441 gataataggc aaacgaagcc aggaattccg cggtgctgaa gactacttcc cgccatttct
     1501 ctagcgtttc ccggtttggc ttttgacgat attccatctc tgcaaagtag ataggatgag
     1561 gttgttgcca tatcagaaaa gcatgggcaa cgcctggcca ctccacgtta ttgtatgcag
     1621 tacattttgg ccattttgtt ccctgaaagc cttcactctt agcccttagc ctggccgttt
     1681 ccaggaaatc ttgataaata cccaggctct tgtctgccag ctcctgatta tcccataaag
     1741 cccaatgtgc cagatgccac cagatcatct caaaatggaa gcggccatac cagccgttgt
     1801 tgtttaccag cccgctttct tgcggaggta aggatcccga ctcattcgcc ttcatcaggt
     1861 agcgggataa aacgatgcgt ctctccagtt caaaccaacg ttcgtcttcg ctctcggaga
     1921 gatcgaccgc tgctcccgat tcccaaaatt gcttccatgc tttcctgcta gccgaaagtc
     1981 cttcttttgc tgtgacaggc ttctcttgaa tctgtttggg tgagaactca caggtgaatg
     2041 ccatcttgtt tccctcggga gttagcctat aagtgtgagg attatcttct gcctcggaaa
     2101 agatacaggg ggtatcccag gacaacgcag agtaatattt ttcctgatct agctcccggg
     2161 taaaaataac cttttgcttg gttcctgtgt aggtggttgc atgtctgtcg ggttcatccc
     2221 aaatgccgat ataatttcct ttcctgctgt tgtgggcaga agctccggga aaggtgatct
     2281 ttacagccaa cctcttgagg tcgataaggg gtgattctac tgtcacggcg atcaggtctc
     2341 tatcgggatg gcaggcggta tatacctgta caggaatatc atcaatctca aaataactgg
     2401 agatgatgcc gctccaaaga tccacttcct gcagacaatt ctgcaggtcg gtcatcgcta
     2461 cttccttgcc ctccgcaccc aacaatgaga aagcgatcct ggccaggttg atccgatgcg
     2521 ggttgcccac cagccattcg gta
//
LOCUS       DAOSDR010000423         2532 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrimonas sp. isolate AMR_MDS_1826
            NODE_14351_length_2532_cov_4.430763, whole genome shotgun sequence.
ACCESSION   DAOSDR010000423 DAOSDR010000000
VERSION     DAOSDR010000423.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863355
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrimonas sp. (wastewater metagenome)
  ORGANISM  Petrimonas sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Dysgonomonadaceae; Petrimonas.
REFERENCE   1  (bases 1 to 2532)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2532)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:10:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,720
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 35
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2532
                     /organism="Petrimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14351_length_2532_cov_4.430763"
                     /isolate="AMR_MDS_1826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2023866"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..246
                     /locus_tag="PLI30_12800"
     CDS             <1..246
                     /locus_tag="PLI30_12800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HOI80470.1"
                     /translation="SSIPATTENIKLERRREFVGEGMRFWDIVRWGDTALLTENLTEY
                     NSVRSWNDNWKYLPIPQSEIDKTAGTEFALQQNPGYN"
     gene            269..1774
                     /locus_tag="PLI30_12805"
     CDS             269..1774
                     /locus_tag="PLI30_12805"
                     /inference="COORDINATES: protein motif:HMM:NF040138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HOI80471.1"
                     /translation="MKNKIKQLSGMLLFLFIMAACSPQELNDYGLDSMATLTDDQVSF
                     TQTVSATSDNMVTFTSTTQLPTNSVYTLRWDLGNGSTGNKASATGIYPFAGDYTVTLS
                     IHFPDGSVAKKSVVVSFEDNDYSLVDTPAYRNLTGGADDADGKTWVFDQHNNFAAEVA
                     AATGFAISGHMGLGPINSFGQSWWGAAANDKASWTLYSYKFTFIQNGVQLKIENSGDG
                     YGRKAVSSAAGFNVTGTSGDDAFFPYPGGDYTFSIDESGTHPKLTLSGNAFMGYYCGH
                     QEYEIVYQTEEVMALVVHNQVEQQDWCFVFCREDLNVPAPPIAKELKAIPLSEDFEGD
                     EILAFKQEDMGGAIKSAVIGNPVPLPINESSKVYRYWKSTGFYSNLSFTAPDYKFDLT
                     TQNKVRVKVFIPSFNDYTTENAVAGDWIANKKLLPQLAVKLQDGDHPAPWEGQTEIVK
                     ANLELDKWQELEFDFSTVANREDYDRIVIQFGAEGHAGPGFFYLDDFEFSE"
     gene            1843..>2532
                     /locus_tag="PLI30_12810"
     CDS             1843..>2532
                     /locus_tag="PLI30_12810"
                     /inference="COORDINATES: protein motif:HMM:NF040218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BACON domain-containing carbohydrate-binding
                     protein"
                     /protein_id="HOI80472.1"
                     /translation="MNTIWIALALMLTACGGDDPSPITPKPDVEVKWEITPATTQFSA
                     KGEIKNIVVTANGDWTATSGQSWCSLTPDKGGNGQTLVKISATANPKEEVRNAEITFT
                     SGKYSQKYTVSQDAGTIGNYVPTGYNLVWAEEFDAARLSSGKPALPDTQKWWYENLPK
                     GTVNNELQTYVGGFRGTDTVAAIHDGTLKIIARKSGTDIISARINTIESWTYGYFEAR
                     LRFPGGKGTWPA"
BASE COUNT          675 a          599 c          677 g          581 t
ORIGIN      
        1 agttccattc ccgccaccac cgagaacatt aagctagaac ggcgtcgtga gtttgtgggc
       61 gaagggatgc gcttctggga tattgtccgc tggggcgaca ctgcgttgct gactgagaac
      121 ctgaccgagt acaacagcgt gcggagttgg aacgataatt ggaagtacct gcccattccc
      181 caatcggaaa tagacaagac ggccggtact gagtttgcgc tgcaacagaa tccgggatat
      241 aattaaccac atagaatatc gacgcattat gaagaataaa ataaaacaac tatcgggaat
      301 gctgctgttt ctttttatca tggcagcatg cagtccgcag gaactgaatg attatggcct
      361 cgacagtatg gccaccctga cggatgatca ggtgtctttt acccagactg tatcggcaac
      421 cagtgacaac atggtcactt ttaccagcac tacccaactt cccaccaact ctgtctatac
      481 ccttcgctgg gatctgggaa atgggtccac cggaaacaaa gcttctgcca ctggaattta
      541 tcccttcgcc ggagattata cggttaccct ctccattcat tttcccgatg ggagcgtagc
      601 taaaaagagc gtggtggtca gttttgaaga caacgattac agtcttgtgg atacaccggc
      661 ttacaggaac ctgaccggtg gtgctgatga tgccgatgga aagacctggg tattcgacca
      721 gcacaataat tttgcggctg aagtggctgc agctaccggt tttgcaatca gcggacatat
      781 gggtctggga cctatcaact cattcggaca gtcttggtgg ggagccgctg caaacgataa
      841 ggcctcttgg acactctatt catataagtt tacgttcatt cagaacggtg tgcagctaaa
      901 gattgaaaat tccggtgacg gatacggaag aaaagctgtt tcgtccgccg caggatttaa
      961 tgttacggga acgagcggcg atgatgcttt tttcccctat ccgggtggtg actatacgtt
     1021 ctctatcgac gagagcggta cgcatcccaa gctgaccctc tccgggaatg cttttatggg
     1081 ttattattgc ggccatcaag aatacgaaat cgtgtatcag accgaagagg taatggcatt
     1141 ggtagtgcat aaccaggtgg aacaacaaga ctggtgtttt gttttctgcc gtgaagacct
     1201 caatgtgcca gcgccgccga tagccaagga gctgaaagcg attccgctga gtgaagattt
     1261 tgaaggggat gagattctgg ctttcaaaca ggaagatatg ggaggggcga tcaaaagtgc
     1321 cgttattggt aatcctgtgc cgcttcccat caatgaatca tcgaaagtgt accgttactg
     1381 gaaatcgacc ggtttctaca gtaacctttc cttcactgca ccggattaca agttcgatct
     1441 tacgacgcag aacaaagtca gggtgaaggt ctttattccc tccttcaacg attataccac
     1501 agaaaatgcg gtggctgggg actggattgc caacaaaaaa ttgttgcctc agctggctgt
     1561 gaagctgcag gatggcgatc atccggcccc gtgggaagga cagacagaaa tagtgaaagc
     1621 aaacctggaa ttggataaat ggcaggagtt ggaatttgat ttttctactg tagccaatcg
     1681 ggaggattat gaccggatcg ttatccagtt tggagccgaa ggacatgccg ggccgggttt
     1741 cttctacctg gatgattttg aatttagtga ataatacgga ttaaacgata cgagaggctg
     1801 ccgggtcaca taccggcagc cttttcaacc cttatgaaac gaatgaatac aatatggatc
     1861 gcgctcgcct tgatgctgac agcctgcggc ggagatgacc cgtcgcccat cacccctaaa
     1921 cccgatgttg aggtgaaatg ggagataaca ccggcaacca cacagttctc cgcaaaggga
     1981 gagatcaaaa acattgtcgt gacggccaat ggcgactgga cagcaacctc tggccagtcc
     2041 tggtgcagcc tcacacccga caaaggggga aacggacaga ccctggtaaa gatcagtgca
     2101 acggccaatc cgaaagagga ggtgcgaaac gcagaaatca cttttacttc cggaaagtac
     2161 agccagaagt atacagtttc gcaggacgcc ggcaccatcg gaaattatgt gccgacaggt
     2221 tataacctcg tctgggcgga agagtttgac gctgcccggc taagtagcgg taagcctgct
     2281 ctacccgaca cgcaaaaatg gtggtacgaa aaccttccca agggtacggt caacaatgaa
     2341 ctgcagactt atgtgggggg attcaggggc accgacacgg tggcggcaat ccacgatggt
     2401 acgctgaaga tcatcgctcg gaaaagcgga accgatatca tctcggcgcg catcaatact
     2461 attgagagct ggacctacgg ttacttcgag gctcgattgc ggttcccggg cggtaagggt
     2521 acctggcctg cc
//
LOCUS       DAOSDR010000424         2532 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrimonas sp. isolate AMR_MDS_1826
            NODE_14356_length_2532_cov_2.712152, whole genome shotgun sequence.
ACCESSION   DAOSDR010000424 DAOSDR010000000
VERSION     DAOSDR010000424.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863355
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrimonas sp. (wastewater metagenome)
  ORGANISM  Petrimonas sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Dysgonomonadaceae; Petrimonas.
REFERENCE   1  (bases 1 to 2532)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2532)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:10:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,720
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 35
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2532
                     /organism="Petrimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14356_length_2532_cov_2.712152"
                     /isolate="AMR_MDS_1826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2023866"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..730
                     /locus_tag="PLI30_12815"
     CDS             <1..730
                     /locus_tag="PLI30_12815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HOI80473.1"
                     /translation="RENWSSDAGLTAYDGLDTSEGGVLLRYSFEEAYLQQKRKRKPVD
                     VTSPVFFLSHSIGFVHLPEGGATYHRTEFSAQKRFYLGYAGRLDAVGEFSKVWNQVPF
                     PLLVYPNQRYKHHIENNAFFLNRALEFVADEQYTIRTTFVGDDFLLAKIPLLDDLGAK
                     ELISLRASYGRLSSKNDPLLSPSSLIYDFPPVSYRYGSAPYVEGTVGITNLLGLLRVE
                     YVHRFTYRDHPGALLGRVRVDVTL"
     gene            759..2441
                     /locus_tag="PLI30_12820"
     CDS             759..2441
                     /locus_tag="PLI30_12820"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HOI80474.1"
                     /translation="MVRIIIHIVVITIGVFFLGGCKPQPEAPQLLADAQRFMENNPDS
                     AMLLIDSLFYPEKSLRHEDYMRYLVLHVQARYKTNRPVADDTLVFRARDHYSDRLDKE
                     PRMAALAWFYSGCVYRERGDYEKAMLHYKKAETYATGTADTALQGLIQYNLGDLFAVQ
                     DLFSKAQENYKAAARFYGEHPEKEAYSLSAVGRMFLLDKNPDSAFFYFQKGLEAAKPT
                     NDKVLQSLLAQNTGVAYQQIGQYAEAERYFRQSYRYNRDKEDQARYYLNLAKLFAQTE
                     QMDSAAFYAGKLKNSVESSDDNYLKASAYKFLADWEKARGRDNEAFAYQNKRIHSLNR
                     IMDDRKDQSVYEIEQKYNYGQKQNFYNQRFIRFQQWLMGLMGVLLVVVVSLLFLFWRM
                     LKERAAKLRMQENVNTLRATTIDLLSVEEESRLKEKVLKETLQWKLDVLKNTMHLRYM
                     MSEREQKTYQPLFDSLDKILFDQNTKTPGMDIRQTVEHLNPSLRLFIRQQYPTLSETE
                     YNVCLLSYASLSVQEVAFVLNQSEHTVYKARTRLNKKIGSDFYSVLRRALSL"
BASE COUNT          689 a          559 c          624 g          660 t
ORIGIN      
        1 cagggagaac tggagttccg acgccgggct taccgcatac gatgggctgg acacttccga
       61 gggtggggtg ctgctgcgct actctttcga agaggcctat ttacagcaaa aacgcaaacg
      121 aaaacctgtc gatgtgacaa gtcccgtctt ttttctttca cactccatcg gcttcgtgca
      181 tttacccgaa ggaggtgcta cataccacag aacggagttc tccgcacaaa aacgtttcta
      241 cttgggttac gccgggcgac tggatgctgt aggggaattt tcgaaagtgt ggaaccaggt
      301 gccctttccc ttgctggtct atcccaacca acggtacaaa catcatatcg agaacaacgc
      361 gttcttcctg aaccgggccc tggagtttgt tgccgatgag cagtatacga ttcggacgac
      421 ttttgtaggc gatgatttcc tgttggcaaa gattccactg ctggacgatc tgggagcaaa
      481 agagttgatc tcgttgcgcg cctcttacgg taggctgagc agcaaaaacg acccgttgct
      541 ttccccctct tctctcatct acgattttcc gccggtatcc taccggtacg gctcagcccc
      601 ctatgtggag ggaaccgttg gaatcacgaa tctcctggga cttttgcgtg tggaatacgt
      661 tcatcggttc acgtatcgtg atcatcccgg cgctttactg ggcagggtgc gggttgacgt
      721 gacattgtaa gaattttttt atatctttga gattgcatat ggtacgtatt atcatacata
      781 tcgtagtaat cacaatcggc gtttttttcc tgggaggctg taaaccccag cccgaagcgc
      841 cgcagttgct ggcagatgcc cagcgcttta tggaaaataa ccccgacagc gccatgctgc
      901 tgatcgattc ccttttctat cctgaaaaaa gcctccgcca cgaggattat atgcgttatt
      961 tggtgttgca cgtgcaggca cgttacaaaa caaaccgccc tgttgctgat gatacgttgg
     1021 ttttccgggc gcgtgaccat tattccgacc gacttgataa agaaccccgc atggccgccc
     1081 tggcctggtt ctatagcggc tgcgtatatc gtgagcgggg agattacgaa aaggctatgc
     1141 tgcattataa aaaggcggaa acatatgcca cgggaacagc cgataccgct ttgcaagggt
     1201 tgatacaata taatctggga gatttatttg cggtgcagga tttattttcc aaagcacagg
     1261 aaaactataa agcggcagcg cgattttacg gtgaacatcc cgaaaaagag gcttatagtt
     1321 taagtgccgt ggggaggatg ttcctgctgg ataaaaatcc tgacagcgca tttttttatt
     1381 ttcaaaaggg cctggaggct gcaaaaccca ctaacgataa ggtattgcaa agtttattgg
     1441 cacaaaatac cggcgtggcg tatcagcaaa tcggacaata tgctgaggca gaaaggtatt
     1501 tccgtcaatc ctaccgatat aaccgggaca aagaggatca agcccggtac tatttgaatc
     1561 tcgcaaaatt atttgcgcaa acggaacaga tggattcggc cgctttctat gccggaaaat
     1621 taaagaacag tgttgagtca tccgatgaca attacctcaa ggcgagcgct tacaaatttt
     1681 tggctgactg ggaaaaagca agggggcgtg acaatgaagc tttcgcctat cagaacaaac
     1741 ggatacatag ccttaaccgg atcatggacg atcggaaaga tcagtcggtt tacgaaatag
     1801 aacaaaaata caactacggg caaaagcaga acttctataa tcagcggttt atccgttttc
     1861 agcaatggct gatgggattg atgggggtat tgctggttgt ggtagtctcc cttttatttc
     1921 tcttctggcg gatgctgaag gaaagagcgg ccaagctgag aatgcaagag aatgttaata
     1981 cgttgcgtgc aaccacgata gatctgttgt ccgtagagga agaaagccgc ttgaaagaaa
     2041 aggtattgaa agaaacattg cagtggaaac tggatgtgtt gaagaataca atgcacctga
     2101 gatacatgat gagcgaaagg gaacaaaaaa cttatcaacc tttatttgat agcctggata
     2161 agattctatt cgatcaaaac acaaagacgc ccggaatgga tatacgacaa accgtggaac
     2221 accttaatcc ctcactaaga ctttttataa ggcagcaata tcctacactt tctgaaaccg
     2281 aatataatgt gtgtctgttg tcgtatgcca gcctgagcgt acaggaagtc gcttttgtcc
     2341 tcaatcagag cgaacatacc gtttacaagg cccggaccag gctcaataaa aagataggct
     2401 ccgatttcta ctcggtgctg cggagagcgc ttagccttta accttatttg ttaaaattct
     2461 gtattgctta gtatccttga tttgatgtat cctgtttatc agtagattaa ctggatggtg
     2521 tagttaattt tt
//
LOCUS       DAOSDR010000425         2530 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrimonas sp. isolate AMR_MDS_1826
            NODE_14373_length_2530_cov_4.091717, whole genome shotgun sequence.
ACCESSION   DAOSDR010000425 DAOSDR010000000
VERSION     DAOSDR010000425.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863355
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrimonas sp. (wastewater metagenome)
  ORGANISM  Petrimonas sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Dysgonomonadaceae; Petrimonas.
REFERENCE   1  (bases 1 to 2530)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2530)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:10:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,720
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 35
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2530
                     /organism="Petrimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14373_length_2530_cov_4.091717"
                     /isolate="AMR_MDS_1826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2023866"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..289)
                     /locus_tag="PLI30_12825"
     CDS             complement(<1..289)
                     /locus_tag="PLI30_12825"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006801191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HOI80475.1"
                     /translation="MTDTPQTIAIIVALDEQNAIGSNGDLLCHLPSDLKHFKKITTGY
                     AVIMGRKTYESLPKGALPNRTNIVITSGEPENYPGCTVVRSVEEALSRCSNQ"
     gene            complement(286..1080)
                     /locus_tag="PLI30_12830"
     CDS             complement(286..1080)
                     /locus_tag="PLI30_12830"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011965387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004799 - thymidylate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HOI80476.1"
                     /translation="MKQYLDLLQRVLDEGVRKEDRTGTGTISIFGHQNRFRMNDGFPV
                     LTTKKLHLKSIIHELLWFLNGDTNVKYLNDNGVRIWNEWADEDGNLGHIYGYQWRSWP
                     DYNGGHIDQISQIVNTIKNNPDSRRIIVSSWNVADIPNMKLPPCHAFFQFYVADGKLS
                     LQLYQRSADIFLGVPFNIASYALLLKMMAQVTGLDEGDFIHTFGDAHIYLNHLEQVKL
                     QLSREPRPLPEMKINPDVKDIFSFRFDDFELVNYDPHPHIKGEVAV"
     gene            1285..1872
                     /locus_tag="PLI30_12835"
     CDS             1285..1872
                     /locus_tag="PLI30_12835"
                     /inference="COORDINATES: protein motif:HMM:NF017853.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF937 domain-containing protein"
                     /protein_id="HOI80477.1"
                     /translation="MDLSELLSGPIGQTVINNVSKQLGMDERQAANAISVALPAILGG
                     LQRNVQSREGAQSLDNALNDARHDGSLLENLASLLGGDTQPISQDGGKILGHIFGDDK
                     PAVEQGISQKTGLSLQKIGTLLALLAPVVMAYLGKEKRQSNTNAGGLGDLLGGLLGGG
                     QSQRRGGGGLMDMVGGLLDKNRDGNVLDDILGMIK"
     gene            2027..>2530
                     /locus_tag="PLI30_12840"
     CDS             2027..>2530
                     /locus_tag="PLI30_12840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI80478.1"
                     /translation="MLTLMKRNFFTSLLAGFLLSGMIFMGTSCSNDDYLKDYEIQKMI
                     DESLNGQWKIKNIAVDKDHWVWNASMSQYEAIYDLPELTKSIYEEGAVLGYIFLGQQG
                     VDEVQKPLPFVNTYFGGNDTEGKPIYFTETISYDVQYKDNGKSTVAFFIKDSQLENDP
                     DAPQNYNF"
BASE COUNT          729 a          490 c          583 g          728 t
ORIGIN      
        1 cctgattgct gcaacgagaa agcgcctctt cgacagagcg gacaaccgtg catccggggt
       61 agttctccgg ttcaccggag gtaatcacga tattggtacg gttgggtaat gctcctttgg
      121 gtaaagattc ataggttttc cggcccatga tcacagcgta tccggtggtg atctttttaa
      181 agtgcttcag gtcgctgggt agatggcaga gcagatcgcc gttgctacca atggcatttt
      241 gttcatcgag agcaacgatg atggctatgg tttgaggagt gtctgtcata cggctacttc
      301 accttttata tgtggatgcg gatcgtaatt taccagttca aaatcgtcga aacggaaact
      361 gaaaatatcc ttcacatcgg gattgatctt catctcgggc aacggtcgcg gttcgcggga
      421 aagttgcagt tttacctgtt ccaggtggtt gaggtagata tgggcatcgc caaaggtgtg
      481 gatgaaatca ccctcatcca gcccggtaac ctgtgccatc attttcaaca gcaacgcata
      541 ggacgcaatg ttaaagggta cacccagaaa aatatcggcg ctgcgctgat agagttgcag
      601 gctcagtttt ccatcggcta cgtagaactg gaagaaggcg tgacagggcg gaagcttcat
      661 gttgggaata tcggccacgt tccacgaact tacgatgatg cggcgagaat cagggttatt
      721 tttaatcgtg ttgactatct gagatatttg atcgatatgt cccccgttat agtccggcca
      781 cgaacgccac tggtaaccgt atatatgtcc gagattccca tcctcgtccg cccattcgtt
      841 ccagatacgt acaccgttgt cgttcaggta ttttacgttg gtatccccgt taaggaacca
      901 caacaattca tgtataatcg attttaagtg aagttttttg gtagtgagaa cgggaaatcc
      961 gtcgttcatc cggaaccggt tctggtgccc aaaaatactg atggtacccg ttccggtacg
     1021 gtcttctttc cgcacacctt catccagcac gcgctgaagt aaatctaaat attgtttcat
     1081 tcttaaaaga gtttcacaca aaaatacaag tttattttct tatctcaaac tgtattatcc
     1141 gaaagatagc cggatgaaaa ttccaaaata gccctgtcaa gacgtattgt tgattttttc
     1201 catagattct tttttttaca aaactaaatg ggtgttacgg tgttaaataa atatctttat
     1261 aaaattttta atcaatttaa caacatggac ttatcggaat tattaagcgg tccaatcgga
     1321 caaacggtta taaacaatgt atccaaacag ttgggtatgg atgaaagaca ggctgcaaac
     1381 gctattagtg ttgctctgcc ggctatcttg ggtggactgc aaagaaatgt tcaatcccgg
     1441 gaaggtgcac aaagtctgga caatgccttg aacgatgcaa ggcatgacgg aagcctgttg
     1501 gaaaacctcg ctagcctttt aggcggcgat actcagccta tcagtcagga tggcggaaaa
     1561 atcctggggc atatttttgg agacgacaaa ccggctgtgg aacagggaat ctctcaaaag
     1621 accgggctca gcttacaaaa gattggtacg cttctggctc tcttggctcc tgttgtgatg
     1681 gcctatttgg gaaaggaaaa acgtcagtct aacaccaacg ccggcggact tggagacctg
     1741 ctgggcggtt tattgggtgg agggcaatcc caaagacgtg gcggaggtgg attaatggat
     1801 atggttggag gcttgctgga caaaaaccgc gatggaaatg tcctcgatga tattttggga
     1861 atgattaaat aaaagactcc tcatcctgtt tttttctgta aaaagtcctg aataaggact
     1921 ttttttgctt atttttgaag caaataaaca attgctttaa aaaatacgtt ttaacagtta
     1981 aacgagtgca gtttttttca gtcaatgaat caagaaaaaa cttttaatgc ttacacttat
     2041 gaaaagaaat ttttttactt ctctccttgc aggattttta ttatcaggaa tgatttttat
     2101 ggggacttca tgcagtaatg acgactattt aaaagattat gaaattcaga aaatgataga
     2161 cgaatcgctt aacggtcaat ggaagataaa gaatattgca gttgataagg accattgggt
     2221 atggaatgca tccatgagcc aatatgaggc tatatatgat ttacctgaat taaccaaatc
     2281 catttatgaa gaaggtgctg ttttgggtta tatatttttg ggccagcaag gagtagacga
     2341 agtacaaaaa ccccttcctt ttgtaaatac atattttggt ggtaacgata cagagggtaa
     2401 accaatttat tttactgaaa ccatcagtta tgacgttcaa tataaagata acggtaaaag
     2461 tacggttgct ttctttataa aagattcgca attggaaaac gatccagatg caccgcaaaa
     2521 ctacaatttc
//
LOCUS       DAOSDR010000426         2501 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrimonas sp. isolate AMR_MDS_1826
            NODE_14605_length_2501_cov_3.493050, whole genome shotgun sequence.
ACCESSION   DAOSDR010000426 DAOSDR010000000
VERSION     DAOSDR010000426.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863355
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrimonas sp. (wastewater metagenome)
  ORGANISM  Petrimonas sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Dysgonomonadaceae; Petrimonas.
REFERENCE   1  (bases 1 to 2501)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2501)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:10:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,720
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 35
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2501
                     /organism="Petrimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14605_length_2501_cov_3.493050"
                     /isolate="AMR_MDS_1826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2023866"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..170
                     /locus_tag="PLI30_12845"
     CDS             <1..170
                     /locus_tag="PLI30_12845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006842256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lipoprotein-releasing system ATP-binding protein
                     LolD"
                     /protein_id="HOI80479.1"
                     /translation="SLDTDNKAELHSLFFELRSQLNQTFIIVTHDEQLASITDRTIHM
                     MDGRIVEKISD"
     gene            223..2328
                     /locus_tag="PLI30_12850"
     CDS             223..2328
                     /locus_tag="PLI30_12850"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009316733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HOI80480.1"
                     /translation="MVMATTGLLLSCNNEQKSDTDSKNLGEAIGRSKIKVENGLITPE
                     VLYSFGRIGSVEVSPDKSKILYQVTYVDIKQNKTNPELFVMNTDGSDKKQLTVTNARE
                     SNPQWISGGKKVAFISNESGTSQIWIMNPDGSGMKKITDVEEGITGFVFSPDNKKVLF
                     IKNVKVGEAIKDKHPDLPEASGRIVDNLMYKHWDSWVEEIPHTFVADFSDDKVSNIID
                     LLQDEPYEAPMLPFGGIEQLAWSPDGKTIAYTSRKKTGKEYAVSTNSDIFFYDVESGK
                     TTNMTEGMMGYDVNPQFSPDGSKLAWQSMERDGYEADKNRLFVMDLATGDKTYVTENF
                     DYNTDAFIWNTDNKTLYLVACVEAKTQLFSADIRSKQISPITKGKHDYASVALGNGKL
                     IAKRHSMSQPDEIYSVELTNGQATEISFENKHILEQLTMGETEERWIETTDGKQMLTW
                     VVYPPDFDSTKTYPALLYCQGGPQSTVSQFWSYRWNLQMMAANGYIVVAPNRRGLPGF
                     GQEWNEQISGDYGGQNMKDYLSAIDALAQEPYVDETRLGCTGASYGGFSVYWLAGNHD
                     KRFKAFLSHAGIFNLEAQYLETEEMWFANWDMGGPYWDKLNAVAQKTYATSPHRFVDK
                     WDTPIMITHGELDYRILASQGMMAFNAAQLRGIPSRMLIFPDENHWISKPQNGILFQR
                     EFFRWFDQWLKPGGKVNTQ"
BASE COUNT          767 a          500 c          627 g          607 t
ORIGIN      
        1 gaagccttga taccgataat aaagccgaac tgcattcatt gttttttgaa cttcgcagcc
       61 agctaaatca aacgtttatc attgtaacac atgacgaaca actggcatcc attacagatc
      121 gaaccattca tatgatggat gggagaattg ttgaaaagat ttcagattaa aaatcaaatg
      181 gcaaaaaaca agtattctat gaaactaaaa tcgtttttta ttatggtaat ggcaacgact
      241 ggactgttac tctcatgtaa caacgaacag aaatcggata ctgattcaaa gaacctgggg
      301 gaagctatag ggagatcaaa aataaaagtg gagaatgggt taataacccc ggaggttttg
      361 tatagttttg gacgtatagg aagtgtggaa gtgtctccgg acaagtcgaa aatactttat
      421 caggttacct acgtcgatat taaacagaac aagaccaatc ccgaactttt tgtgatgaat
      481 actgatggga gcgataaaaa acaattaaca gtcaccaatg ccagagagag caatccgcaa
      541 tggatctcgg gggggaagaa ggtcgcgttc ataagcaacg aaagcgggac atcacaaata
      601 tggatcatga atcccgacgg tagcggaatg aaaaagataa ccgatgtgga ggaaggaata
      661 actggttttg ttttttcgcc agacaataaa aaggtgctgt tcatcaagaa cgtaaaagtg
      721 ggggaggcca taaaagataa gcatcccgat ttgccggagg catcgggcag gatcgtggat
      781 aacctgatgt acaagcattg ggacagttgg gtggaagaaa tcccgcacac gtttgtggct
      841 gatttcagcg acgataaagt atcgaacatt attgatttac tccaggatga gccttatgag
      901 gcccccatgc tgccttttgg cgggatagaa caactggcct ggagccccga tgggaaaacc
      961 atcgcatata cctcccgtaa aaaaacagga aaggaatacg ccgtttccac caattccgat
     1021 atcttttttt acgatgtgga aagcggaaaa acaacaaaca tgaccgaagg aatgatgggt
     1081 tacgatgtga atccgcagtt ttcgcccgac ggttcaaaac ttgcctggca aagcatggag
     1141 cgtgacggat atgaggccga taaaaacagg ctgtttgtga tggacctggc aacgggtgat
     1201 aagacgtatg tgacggagaa tttcgactac aataccgatg catttatctg gaacacggat
     1261 aataaaaccc tttatttggt ggcttgcgtt gaagccaaaa cgcagctgtt ctctgccgat
     1321 atccggtcga aacagatttc gccaataacc aaaggcaagc acgactatgc atccgttgcg
     1381 cttggaaacg gaaagcttat agcaaaacgc cattccatgt cgcaacccga cgagatatac
     1441 tccgttgaac ttaccaacgg gcaagccact gaaatttcgt ttgagaacaa acacatcctg
     1501 gagcagctga ccatgggcga aacggaagag cgctggattg agactactga cgggaaacag
     1561 atgttaacct gggtagttta tccgcctgac ttcgattcta caaagactta tccggcattg
     1621 ttgtattgtc agggcggtcc tcaaagcacg gtaagccagt tctggtctta ccggtggaac
     1681 ctgcaaatga tggctgccaa cggatatatt gtggtagcgc ccaaccgtcg tggattgccg
     1741 ggattcggac aggaatggaa cgaacagatc agcggtgatt acggtgggca gaacatgaaa
     1801 gattatctct ccgccatcga tgctctcgct caagaaccgt atgttgatga aacccgctta
     1861 ggctgtaccg gggcaagtta tggcggcttt tccgtttact ggcttgccgg taatcacgac
     1921 aagaggttta aagcattctt gtcgcatgct ggaattttta atctggaagc gcaatatctc
     1981 gagaccgaag agatgtggtt tgccaactgg gatatgggag ggccttattg ggataaatta
     2041 aatgcagtag cgcagaaaac atacgctacg tcccctcatc gctttgtgga taaatgggac
     2101 acgcctatta tgattacaca cggcgaactt gattatcgta tcctggcgtc acaaggaatg
     2161 atggcgttca atgcggcaca acttcgtgga attccttcgc ggatgcttat ttttcccgac
     2221 gagaatcatt ggatttcgaa gccgcaaaac ggaattttat ttcagcgtga atttttcagg
     2281 tggttcgatc aatggttaaa accaggaggg aaggtaaata cacaataatc agtttcgata
     2341 acgagtgcaa gaaaaaggaa aaaaatacgg tgaaatattt gcaggtttta aaaatatagc
     2401 tacctttgca ctcgctatcg aaaacgattt gcaatgcgaa cggtgtggta gttcagttgg
     2461 ttagaatacc tgcctgtcac gcagggggtc gcgggttcga g
//
LOCUS       DAOSDR010000427         2498 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrimonas sp. isolate AMR_MDS_1826
            NODE_14633_length_2498_cov_3.698322, whole genome shotgun sequence.
ACCESSION   DAOSDR010000427 DAOSDR010000000
VERSION     DAOSDR010000427.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863355
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrimonas sp. (wastewater metagenome)
  ORGANISM  Petrimonas sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Dysgonomonadaceae; Petrimonas.
REFERENCE   1  (bases 1 to 2498)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2498)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:10:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,720
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 35
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2498
                     /organism="Petrimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14633_length_2498_cov_3.698322"
                     /isolate="AMR_MDS_1826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2023866"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..183
                     /locus_tag="PLI30_12855"
     CDS             <1..183
                     /locus_tag="PLI30_12855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI80481.1"
                     /translation="HPVVFVVLVTFPLAIKNIKLMKTATVDNLVKIQFLDTHTAKLVL
                     MFSVLLSAANFIAPFV"
     gene            334..2484
                     /gene="scpA"
                     /locus_tag="PLI30_12860"
     CDS             334..2484
                     /gene="scpA"
                     /locus_tag="PLI30_12860"
                     /EC_number="5.4.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016273010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004494 - methylmalonyl-CoA mutase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase"
                     /protein_id="HOI80482.1"
                     /translation="MKPDFKSIDFKSAGFEATDVAGWAEKNEIKADWLTPEQIPVKPV
                     YTREDLEGMEHLGYAAGVEPFLRGPYSTMYVMRPWTIRQYAGFSTAEESNAFYRRNLA
                     SGQKGLSVAFDLPTHRGYDANNERVVGDVGKAGVSICSVEDMKILFDGIPLNKMSVSM
                     TMNGAVLPILAFYIVAAQEQGANLEEMAGTIQNDILKEFMVRNTYIYPPEFSMKIIAD
                     IFEFTSQKMPKFNSISISGYHMQEAGATADIELAYTLADGIEYLRAGINAGINIDAFA
                     PRLSFFWAIGMNHFMEIAKMRAARLLWAKIVKSMGAKNPKSMALRTHSQTSGWSLTEQ
                     DPFNNVGRTCIEAMAAALGHTQSLHTNALDEAIALPTDFSARIARNTQIYIQEETQIT
                     KQVDPWAGSYYVESLTQELVDKAWALIQEIEKLGGMAKAIETGVPKMRIEEAAARTQA
                     RIDSGAQKIIGVNVCRLEKEDPIDILEVDNTEVRKQQISRLEQLKAGRDNEAVRKALD
                     AITKCVETKQGNLLELSVEAARVRATLGEISDACEKVVGRYNAVIRTISGVYSAESKS
                     DATLEEARAMTEKFARKEGRQPRIMVAKMGQDGHDRGAKVVATGYADCGFDVDMGPLF
                     QTPAEAARQAVENDVHVLGVSSLAAGHKTLVPQVIEELAKLGRPDIIVIAGGVIPAQD
                     YDFLYKAGVAAIFGPGSPVAKSAIQIVKILLGEE"
BASE COUNT          713 a          551 c          639 g          595 t
ORIGIN      
        1 catcctgttg tttttgtggt gctcgttact tttccattgg ccataaaaaa catcaaactc
       61 atgaaaacag ctactgttga caacctggta aaaatacagt ttctcgatac ccacactgca
      121 aaactggtat tgatgttcag cgtgctgtta tcggcggcaa actttatcgc gccgtttgtc
      181 tagacaaaaa gaaacaagaa tcaagacatt gggcttggat tgataggaaa aatagtatac
      241 gattaaagat aaagtacatt tcatacagaa aaaataatat ttaaaagtct tggctcttga
      301 atgtaagagt cctggctcta aaaaagaaaa aacatgaaac ctgatttcaa aagtattgat
      361 tttaaatcgg ctggctttga agcaacagac gttgccgggt gggctgagaa aaatgaaata
      421 aaagccgatt ggcttacacc ggaacaaatt ccggtaaaac ctgtatacac aagggaagat
      481 ctcgaaggaa tggaacatct cggatatgct gccggagtgg agcctttctt gcgcggacct
      541 tattctacca tgtatgtaat gcgtccgtgg actattcgtc aatatgccgg attttctacc
      601 gccgaagagt ccaatgcctt ttatcgccgc aacctggctt cgggccaaaa aggattgtcc
      661 gttgctttcg atctacccac acaccgtgga tatgatgcca acaacgagcg tgtggtaggc
      721 gatgtgggca aagctggggt atcgatttgt tcggtggaag acatgaaaat tcttttcgat
      781 ggaattccgc ttaacaagat gtctgtttcc atgaccatga acggggctgt gttacccatc
      841 ctggctttct acattgttgc ggcacaggaa cagggagcta atctggaaga gatggctgga
      901 acgattcaga acgatatcct gaaagaattt atggtgcgaa acacctacat ctatccaccc
      961 gaattctcca tgaaaatcat tgccgatatt tttgagttta cgtcacagaa gatgccgaag
     1021 ttcaactcta tttccatttc gggataccat atgcaggaag caggagcgac agccgatata
     1081 gaactggctt ataccttggc tgatggtatt gagtacctgc gtgctggaat caacgcgggc
     1141 atcaatatcg atgcttttgc tccccgcttg tcgttcttct gggccattgg tatgaaccac
     1201 ttcatggaaa ttgccaaaat gagggcagcc aggctgcttt gggcgaagat tgtgaaaagc
     1261 atgggcgcga agaacccaaa atcgatggcg ttaagaaccc attcgcagac atcaggttgg
     1321 tcgttgaccg aacaagaccc gttcaacaat gtgggacgta cttgtatcga agctatggcg
     1381 gctgctctgg gacacacgca atcgttgcac acaaatgcat tggacgaggc catcgctctg
     1441 ccgaccgact tctcggcccg tattgcccgt aacacgcaaa tctatatcca ggaagaaacg
     1501 cagatcacca aacaggttga cccgtgggca ggctcctatt acgtggaatc gcttacccag
     1561 gaactggtgg acaaagcgtg ggcgttgatt caggaaattg agaaactggg cggaatggct
     1621 aaggctattg aaacgggtgt tcccaaaatg cgtattgaag aggctgccgc acgtactcaa
     1681 gcccgtatcg attcgggagc acagaagata atcggtgtaa acgtttgccg gctggagaaa
     1741 gaagacccca tcgatattct tgaagtggat aataccgaag tacggaaaca gcaaatttcc
     1801 cggttggaac aacttaaggc cggacgagat aacgaagcgg tgcgcaaagc gctggacgcc
     1861 attaccaaat gcgtggaaac taaacaggga aatctccttg aactatcggt cgaagcagca
     1921 cgggttcgcg ctacactggg cgaaatatcc gatgcctgcg aaaaagttgt aggacgttat
     1981 aatgcagtaa tcagaacaat ttcaggagtg tattcagcag aatcaaaatc agacgccacg
     2041 ctcgaggaag cacgtgcgat gaccgaaaaa tttgccagga aagaaggccg ccagccgaga
     2101 atcatggtgg caaaaatggg acaggacgga cacgaccgtg gagcaaaagt ggttgctacg
     2161 ggttatgccg actgcggttt cgatgtggat atgggaccgc tcttccagac tccggcagaa
     2221 gctgcccgcc aggccgtaga aaacgatgtt cacgtgctgg gcgtatcttc gttggcagcc
     2281 ggacataaaa cgcttgttcc tcaggtcatt gaagaactgg ctaaattagg ccgtcccgat
     2341 attatcgtta tcgccggtgg tgtaattccc gcccaggatt acgatttcct ttacaaggca
     2401 ggggttgccg ctatttttgg cccgggctcc ccggtggcga aatcggccat ccagattgta
     2461 aagatcttgt tgggagaaga atgataggaa ttacaatt
//
LOCUS       DAOSDR010000428         2474 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrimonas sp. isolate AMR_MDS_1826
            NODE_14815_length_2474_cov_3.426623, whole genome shotgun sequence.
ACCESSION   DAOSDR010000428 DAOSDR010000000
VERSION     DAOSDR010000428.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863355
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrimonas sp. (wastewater metagenome)
  ORGANISM  Petrimonas sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Dysgonomonadaceae; Petrimonas.
REFERENCE   1  (bases 1 to 2474)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2474)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:10:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,720
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 35
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2474
                     /organism="Petrimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14815_length_2474_cov_3.426623"
                     /isolate="AMR_MDS_1826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2023866"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..956)
                     /locus_tag="PLI30_12865"
     CDS             complement(<1..956)
                     /locus_tag="PLI30_12865"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HOI80483.1"
                     /translation="MKRIILFLCLVFVGSAIFSQNRILVTGKVTDTSNQGLPGVAVQV
                     KGTLQGVTSNVDGVWTVEAEPDAILVFSFIGYTTVEVPIQGRTTFNVQLKEQAFDVEE
                     VVVVGYGTQKVKDLTSSITTVKSEDLVKTPTGQAMQALQGKVAGLQVVSNGSPGNSPT
                     IRVRGIGSYPGNNNEAPLYVVDGMFFDNIDFLNPTDIASISVLKDASAAAIYGVRAAN
                     GVVLIETKSGGYDQKSEITYDGYYGYQIAQNVLKMANAEQFTTMALESGSLADASFIL
                     NAMQRYGRSRINPNVPDVNTDWYKEILREAAIQNHSLTVSGG"
     gene            1278..>2474
                     /locus_tag="PLI30_12870"
     CDS             1278..>2474
                     /locus_tag="PLI30_12870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI80484.1"
                     /translation="MGTTLKLFAICFMIIGRLLFHPVSCTAFDKSTIPQVKNFTKRQY
                     KASSQNWSVAQDRGGHIYVANSVGLLEFDGSSWKLYPSPNGNIIRAVAVDSLNRIFTS
                     GYQELGYWTRDQFGQLHYSSLKEKAKSFFNPNVEFWDIFISGEKVFFYAFTQLLVFEN
                     ETIKAFNFDSFTNYCTLINGKLLFNEINGGIFEFDNFSAKPFIQHPLLNEKNVRFILP
                     HGKEQYLIGTASHGIFKMNEGALEEWDTPLNDYFRKNQINRGSVTPRGELLIGTILDG
                     ISLLDKNGQLLWKLNSENGLQNNTVLGITIDSEQNIWLALDRGIDLIAYQKDPGHMLF
                     STSNTGAVYSAAHYNGKIYIGTNQGLYFRNQSTPQATFQLVPETQGQVWDIKTISGSL
                     VVGQNSG"
BASE COUNT          748 a          591 c          460 g          675 t
ORIGIN      
        1 cttccaccgg aaacggtaag actgtgattt tgaatagcag cttcacgaag gatctctttg
       61 taccaatcgg tatttacatc cggcacattg gggtttatcc tgcttctccc gtagcgttgc
      121 atggcattca ggataaaact ggcatcggca agcgaaccag actccagggc cattgtggta
      181 aactgctcag cattggccat cttcagtaca ttctgggcaa tttggtaacc ataatagcca
      241 tcgtatgtaa tttccgactt ctggtcatat ccacccgatt tggtttcaat taaaaccacg
      301 ccattagcag cccggacacc ataaatggcc gcagccgaag catctttcag taccgaaatc
      361 gatgcgatgt ccgttggatt caaaaaatcg atgttgtcaa aaaacatccc gtcaactaca
      421 tacaacggtg cttcattatt gttccccgga taggacccga ttccccttac ccgaatcgtc
      481 ggggaatttc ccggagaacc gttactcacc acctgcagtc cggctacttt tccctgtaag
      541 gcttgcatag cctgcccggt tggagtttta acaagatcct ccgactttac cgtagtaatc
      601 gacgaagtaa ggtctttcac cttttgcgta ccataaccaa ccactacaac ctcctcaaca
      661 tcaaaagcct gttctttgag ctgaacatta aaagtggtac ggccttgtat cggcacttca
      721 acagtggtat aaccgatgaa gctaaaaaca agtattgcat cgggttccgc ttcaacagtc
      781 caaacaccgt caacattaga ggttactccc tgcaacgtac cttttacctg caccgcaacc
      841 cccggcaaac cctgatttga agtatctgta acttttccgg taaccagaat cctgttttgc
      901 gaaaaaattg ctgatccaac aaatactaag cataaaaaca aaatgattct cttcatagta
      961 cactggaatt aattagtttt gaaacttcaa tttatgacac ttctgactac tcttcacaat
     1021 cagacaccac actttaattt cttgccctgt tttagaaaca aaaatcaacc tcacttttga
     1081 tacactacta ttaaaaatta atccataagt cactgacaga cataaaaata aaaacaaatc
     1141 tgttttcttg gtttttataa taagcccaac tatatttaca tacaggtgaa gtcatggaat
     1201 gtttaaaaca gtattcaaca tttttgaaag aactaaaaac caaactaaaa aaccgaaaaa
     1261 gatcaactca aaaagaaatg ggaacaacac tgaaactttt tgccatttgt tttatgataa
     1321 tcgggcggct gctattccat ccggtttcat gtacagcttt tgacaaaagc actattcctc
     1381 aggtaaaaaa ctttaccaaa aggcagtata aagcttcaag tcaaaactgg tctgtagcac
     1441 aggaccgggg aggacatatt tatgttgcca acagtgtagg tttactggag ttcgatggct
     1501 catcctggaa actctaccct tctccaaacg gaaatattat ccgggcagtt gcagtagatt
     1561 ccctcaaccg tatttttacc agtggttacc aggaactggg gtactggaca cgtgaccaat
     1621 tcggacagtt gcactactct tccctcaaag agaaggcaaa atctttcttt aatcccaatg
     1681 tcgaattttg ggatatcttt atttctggcg aaaaggtctt cttctatgcg tttacacaac
     1741 ttctggtttt tgaaaacgaa acgatcaaag catttaattt cgatagcttt accaactact
     1801 gcaccttaat aaacggaaaa ctgcttttca atgaaattaa tggcgggatc tttgaatttg
     1861 ataatttttc agctaaaccc tttattcagc acccgctttt aaatgaaaaa aatgtccgtt
     1921 ttatccttcc tcacgggaaa gagcaatacc tgatcggaac cgcttcgcat ggaattttca
     1981 aaatgaatga aggtgcgctg gaggaatggg acaccccgct gaacgactat ttccgaaaaa
     2041 atcagatcaa ccgcggcagt gtgactcccc gtggcgaatt gctcatcgga actattcttg
     2101 acggcatttc tctccttgat aaaaacggac agctcctatg gaaactcaat tcggaaaacg
     2161 ggctacaaaa caacaccgta cttggtatta cgatcgattc tgaacagaac atttggctgg
     2221 cgctcgatcg cggcatcgat ctgatcgctt atcagaaaga tcctggacac atgttatttt
     2281 ccacatcgaa taccggagca gtttattcgg cagcacatta caacggaaaa atttatatcg
     2341 gcaccaacca aggactctat ttcagaaacc agagcactcc ccaggccact ttccagctgg
     2401 ttcccgaaac ccaggggcag gtgtgggata tcaaaaccat ttcaggaagc ctggttgttg
     2461 ggcaaaactc cggt
//
LOCUS       DAOSDR010000429         2462 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrimonas sp. isolate AMR_MDS_1826
            NODE_14911_length_2462_cov_3.559618, whole genome shotgun sequence.
ACCESSION   DAOSDR010000429 DAOSDR010000000
VERSION     DAOSDR010000429.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863355
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrimonas sp. (wastewater metagenome)
  ORGANISM  Petrimonas sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Dysgonomonadaceae; Petrimonas.
REFERENCE   1  (bases 1 to 2462)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2462)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:10:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,720
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 35
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2462
                     /organism="Petrimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14911_length_2462_cov_3.559618"
                     /isolate="AMR_MDS_1826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2023866"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            9..1289
                     /gene="glyA"
                     /locus_tag="PLI30_12875"
     CDS             9..1289
                     /gene="glyA"
                     /locus_tag="PLI30_12875"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009127490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HOI80485.1"
                     /translation="MKRDTQIFEIIEQEKLRQLKGIELIASENFVSDQVMQAMGSVLT
                     NKYAEGYPGRRYYGGCEVVDLGEQLAIDRLKKLFNAEWANVQPHSGAQANAAVFLACL
                     KAGDKFLGLNLSHGGHLSHGSPVNFSGLMFQALEYNVREDNQQVDYDQLESVARAERP
                     KLIIAGASAYSREWDYARIRKIADEIKALFMVDMAHPAGLIAAGLLDNPLKYAHIVTS
                     TTHKTLRGPRGGIILMGQDFENPWGLKTPKGEVKMMSALLDSAVFPGMQGGPLEHVIA
                     SKAVSFGEALDPEFKTYQAQVKRNAAVMAQAFADKGYKVVSGGTDNHIVLVDLRTKFP
                     ALTGKVAEKVLVEADITTNKNMVPFDSRSPFQTSGLRFGTPAITTRGAKESLMGDIVE
                     MIDTVLADVENDKTIASVREKVNATMKQFPLFAW"
     gene            1301..>2462
                     /locus_tag="PLI30_12880"
     CDS             1301..>2462
                     /locus_tag="PLI30_12880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013545752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HOI80486.1"
                     /translation="MILQTDIKFVPGVGPKRAGILNKELDVFTLGDLLRYYPYRYIDR
                     SRMYYVHEIDGSMPYIQLKGKITAFEMFGEGRKKRLVAHFTDGTGFIDLVWFQGIRFV
                     ENKYKANQEYIVFGKPTLFNNRWNIAHPDIDPYLSEADRPDGLMAMYNTTEKMKNHYL
                     NSKAVQKIIENAFGMINGRLPESLSADVVKTAHLMSFHDAVENIHFPRNPVLLRDAEF
                     RLKFEELFFIQLNIVRYAADRKDKLNGFVFKRVGKYLNLFYEKHLPFELTNAQKRVIK
                     EIRKDTASGKQMNRLLQGDVGSGKTLVAVMCMLIAMDNGFQSCLMAPTEILASQHFET
                     FRGLLSDMNVNVALLTGSTRKKERADIHAGLLSGDIHILIGTHALIEDVVQFN"
BASE COUNT          644 a          570 c          680 g          568 t
ORIGIN      
        1 aacaatagat gaaacgagac acacagattt tcgaaattat cgaacaggaa aaattacgtc
       61 agttaaaggg aatagaactt atcgcttcgg agaacttcgt aagtgatcag gtgatgcagg
      121 caatggggtc cgtccttacc aacaagtatg ccgaaggtta tccgggcagg cgttactacg
      181 gcggttgcga ggtggtggat ttaggtgagc aattggctat cgaccgcctg aagaagttgt
      241 tcaatgccga gtgggccaat gtgcaaccgc actcgggtgc acaggcaaat gcagccgttt
      301 ttctggcttg cctgaaagcg ggagacaagt tcctcggact caacctctcg cacggtggac
      361 acctttcaca cggttcaccg gtaaactttt ccggtttgat gtttcaggca ctggaataca
      421 acgtccggga ggacaatcag caagtggatt acgaccaatt ggaatcggtt gcgcgtgccg
      481 aacgcccaaa actgatcatc gccggagcat cagcctattc gcgtgaatgg gattatgccc
      541 gtatccgcaa aatcgccgat gaaatcaagg ccctgttcat ggtggatatg gcgcatcctg
      601 ccggactgat cgccgccgga ttgctggaca acccgttaaa atatgcgcac atagtaacct
      661 ctactaccca caaaacgctt cgcggcccgc gcggaggcat tattttaatg ggacaggatt
      721 tcgagaaccc gtgggggtta aaaacgccga agggagaagt gaaaatgatg tcggcactgt
      781 tggattcggc cgtttttccg ggaatgcagg gaggtccgct ggaacacgtc attgcttcca
      841 aagccgtgtc gtttggcgaa gcgttggatc ccgagttcaa aacctaccag gcacaggtga
      901 aaaggaatgc agccgttatg gcccaggcat ttgcggataa aggttacaaa gtggtgtcgg
      961 gaggtacgga caaccacatt gttttggtgg atttgcggac gaagttccct gcgctgaccg
     1021 gaaaagtggc ggagaaagtg cttgtggaag ccgatatcac gaccaacaaa aacatggtcc
     1081 cgttcgatag ccgcagcccg ttccagacct ccggattgcg ttttggaaca ccggcaatca
     1141 cgacccgtgg agcgaaagaa tcgctgatgg gtgatattgt ggaaatgatc gacacggtgc
     1201 ttgccgacgt ggaaaacgac aagacaatag catcggttcg tgaaaaggtg aatgctacta
     1261 tgaagcaatt cccgttgttc gcgtggtaga attgcaatag atgatccttc agaccgacat
     1321 aaagtttgtt ccgggtgtgg gccccaaacg ggcggggatc ttaaataagg aactcgacgt
     1381 tttcacgctg ggcgatttgc tacgctatta tccttaccgg tatatcgacc ggagccggat
     1441 gtattacgtg cacgaaatcg acggtagtat gccgtacatc cagctcaaag ggaaaataac
     1501 ggctttcgaa atgttcggtg aaggacggaa aaaacggctt gtggcgcatt ttaccgatgg
     1561 tacgggcttt atcgatctgg tgtggtttca gggtatccgg tttgtggaaa ataagtacaa
     1621 ggccaatcag gagtatatcg ttttcggcaa acccacgctt ttcaacaacc gttggaacat
     1681 agcccacccc gatattgatc cctatttgag cgaagccgac cggcccgatg gcctcatggc
     1741 gatgtacaac accacggaga agatgaaaaa ccattacctg aattcgaaag ccgttcagaa
     1801 aatcatcgaa aatgcgtttg gaatgattaa cggcaggctt ccagaatccc tctctgccga
     1861 tgtggtgaaa acggctcatc tcatgtcgtt ccacgacgct gtcgagaaca ttcatttccc
     1921 caggaatccc gtattgctgc gggacgccga gttcaggttg aaatttgagg agttgttttt
     1981 tatccagctt aacatcgtcc ggtatgctgc cgaccggaaa gacaaactca acggctttgt
     2041 ttttaaaagg gtggggaagt acctgaattt gttttacgag aaacacctgc cgtttgaact
     2101 gaccaacgcg caaaaacggg taatcaaaga gatccgcaag gatacggcgt cgggcaagca
     2161 gatgaaccgt cttttgcaag gtgatgtggg tagcggtaaa acgttggtgg cggtcatgtg
     2221 catgctgatt gcgatggata acggtttcca gtcctgcctg atggccccta ccgaaatttt
     2281 ggcgtcacag cacttcgaga ctttccgcgg gctgctgtcg gacatgaacg tgaatgtggc
     2341 gttgctgacg ggctcgaccc ggaaaaaaga acgtgcggac atccatgccg gattgttgtc
     2401 gggagacata cacatcctta tcggcaccca cgcccttatt gaagatgtgg tgcaatttaa
     2461 ca
//
LOCUS       DAOSDR010000430         2456 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrimonas sp. isolate AMR_MDS_1826
            NODE_14956_length_2456_cov_4.386506, whole genome shotgun sequence.
ACCESSION   DAOSDR010000430 DAOSDR010000000
VERSION     DAOSDR010000430.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863355
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrimonas sp. (wastewater metagenome)
  ORGANISM  Petrimonas sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Dysgonomonadaceae; Petrimonas.
REFERENCE   1  (bases 1 to 2456)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2456)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:10:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,720
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 35
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2456
                     /organism="Petrimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14956_length_2456_cov_4.386506"
                     /isolate="AMR_MDS_1826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2023866"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..396)
                     /locus_tag="PLI30_12885"
     CDS             complement(<1..396)
                     /locus_tag="PLI30_12885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008158693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOI80487.1"
                     /translation="MRKKYKQLTSEQRYAIYLGIKNGDSQRTIAESIGVGPSTVSREL
                     GRNKKKHGGYSWRLAHEMAQERKERLPGNRDTPEWIKQKVFRLVRDEWSPKQISGYLE
                     KYKQIRVSHETIYKWIREDKKAGGDLYKHC"
     gene            complement(579..1145)
                     /gene="purN"
                     /locus_tag="PLI30_12890"
     CDS             complement(579..1145)
                     /gene="purN"
                     /locus_tag="PLI30_12890"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005783840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HOI80488.1"
                     /translation="MKRIAIFASGSGSNAENIVRYFSGNDEIEISLILSNKKEAFVHE
                     RAKNLGVPSYTFSKTEFDEAELILETLQQYNIDFIVLAGYLLKVSAPLLKAFPNRIIN
                     IHPALLPKYGGKGMYGDRVHRAVMEAGEKESGISIHYVNEHYDEGDIIFQASCEVLPG
                     DTPGDIAQRVHQLEYKHFPEVIEKIVNA"
     gene            1305..1541
                     /locus_tag="PLI30_12895"
     CDS             1305..1541
                     /locus_tag="PLI30_12895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005779510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HOI80489.1"
                     /translation="MSEVAQRVKSIIVDKLGVEESEVTETASFTNDLGADSLDTVELI
                     MEFEKEFNISIPDDQAEKISTVGDAIAYVEQHAK"
     gene            1562..>2456
                     /locus_tag="PLI30_12900"
     CDS             1562..>2456
                     /locus_tag="PLI30_12900"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016269300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HOI80490.1"
                     /translation="MELKRVVVTGLGAVTPLGNDVKTTWENTVAGKSGAGPITLFDAS
                     LFKTQFACEVKNFDPYELFDRKEARRFDRYSQLALKAAKEAIENSGLDLEKEDTNRIG
                     VIFSAGIGGIKTFEEEVGNYYLTQDKGPRFNPFFIPKMIADIAAGHISMIYGLRGPNY
                     ATVSACASSTNAIADAFNLIRLNKANVIVTGGAEATISASAVGGFNAMHALSTRNDSP
                     ETASRPFSASRDGFVMGEGSSSLILEELEHALARGAHIYAEVAGAGMSADAHHITASH
                     PDGLGAKLVMYNALEDAQMQPE"
BASE COUNT          663 a          551 c          542 g          700 t
ORIGIN      
        1 gcaatgcttg taaaggtcgc ccccggcctt cttgtcctcc cggatccact tgtaaatggt
       61 ctcgtgggaa acccggattt gcttgtattt ctccaaatat ccgctgatct gcttgggaga
      121 ccattcatca cggacaagcc ggaacacctt ctgtttaatc cactccgggg tgtccctgtt
      181 cccgggcaaa cgctcctttc tctcttgtgc catctcgtga gccaagcgcc aggagtaacc
      241 tccatgtttc ttcttgttac gacccagttc cctggacacc gttgaaggac cgacccctat
      301 ggactctgcg atggttcgct ggctgtcacc attttttata cctaaataaa tcgcgtacct
      361 ttgttctgaa gttaactgtt tgtatttttt tctcataagc aacataatag ttaattttag
      421 ggagacttcg gtctcctttt tcttttatgt tgccgcttga ctctcctcgg gggcttcgcg
      481 cccccggccg ttaggcaaac ccccgcggtg tttttcatga ttgttttgaa gaaatcattt
      541 caaaaacaac acccgggtgt tgcacttcta agttgaactt aggcattgac aatcttctcg
      601 atgacttcag gaaaatgttt gtattccagc tggtgtactc tttgggcaat atcgccggga
      661 gtgtcgccgg gaagtacttc gcaggaagct tggaaaatta tatcgccttc atcgtagtgc
      721 tcgttaacat aatgtatgga tatccccgat tccttttcac cggcctccat tactgctcgg
      781 tgaacgcggt cgccgtacat tcctttccct ccatacttgg ggagcaaagc cggatgaata
      841 ttgattatcc tgttggggaa agctttcaga agcggggcag acacttttag cagataaccc
      901 gctaacacga taaaatcgat gttatactgt tgcaacgttt ccaaaatcaa ctccgcttcg
      961 tcaaattccg ttttggaaaa ggtataagac ggaactccca gatttttagc ccgttcgtgc
     1021 acaaaagctt cttttttgtt ggaaagaatc aacgatattt caatttcgtc gttaccggaa
     1081 aaataccgga caatattttc agcgtttgat ccgctacccg aggcaaatat ggctattctc
     1141 ttcataaaac aggtgtgtgt tttgcacaaa aaaacggcaa atgtgcatta tttttgaatt
     1201 tattgattga aaaattgtgt ggttaaagaa aaattgattt actttgcatc cacaaaatta
     1261 aaaagaaaat atcttttatt tactaattta attaataaaa agttatgtct gaagtagcac
     1321 aaagagtaaa atcgatcatt gtcgataaat taggtgttga agagtctgaa gtaacagaaa
     1381 ctgccagctt taccaatgat ttgggagctg attcacttga cactgttgag ttgatcatgg
     1441 aattcgaaaa agaattcaat atctccattc cggacgatca agctgagaaa atttctacgg
     1501 taggtgatgc tattgcttac gttgaacaac acgctaaata aaaaatccac aagtaaaatt
     1561 catggaatta aaaagagtag tagtaacagg tctgggagct gtaacaccgt tgggaaatga
     1621 tgttaaaaca acatgggaaa ataccgtagc gggaaaaagc ggggccggac ctattactct
     1681 tttcgatgca tctttgttca agacacaatt tgcctgtgag gtaaaaaatt ttgatcctta
     1741 cgagcttttt gacaggaagg aggcccggag attcgaccgt tattctcagc ttgccctgaa
     1801 agcagcgaaa gaggcgatag agaactcagg acttgatttg gaaaaagaag acaccaaccg
     1861 tatcggagtt atcttctctg ccggaattgg aggtatcaaa acttttgagg aagaggttgg
     1921 taattattat cttacgcaag ataaaggccc tcgattcaat ccatttttca ttccgaaaat
     1981 gatagccgat attgccgccg ggcatatctc tatgatttac ggactgagag gtcctaatta
     2041 cgcaaccgtt tcggcatgcg catcttctac aaatgccatc gccgatgctt ttaatctcat
     2101 ccgactgaat aaggcaaatg ttattgttac cggtggtgct gaagcgacca tcagcgcctc
     2161 agcagtgggc ggatttaacg caatgcatgc actttctacc cgtaacgatt cgcctgaaac
     2221 ggcttcacgc ccttttagcg ccagccggga cggatttgtc atgggagaag gttcttcgag
     2281 cctgattctc gaagagctgg aacacgctct tgcacgtggc gcgcatatct acgccgaagt
     2341 agctggtgcc ggcatgtcgg ccgatgctca ccacatcacg gcttcccatc ccgacggttt
     2401 gggagccaaa ctggtaatgt acaacgcctt ggaggatgca caaatgcaac ccgaag
//
LOCUS       DAOSDR010000431         2455 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrimonas sp. isolate AMR_MDS_1826
            NODE_14966_length_2455_cov_2.265417, whole genome shotgun sequence.
ACCESSION   DAOSDR010000431 DAOSDR010000000
VERSION     DAOSDR010000431.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863355
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrimonas sp. (wastewater metagenome)
  ORGANISM  Petrimonas sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Dysgonomonadaceae; Petrimonas.
REFERENCE   1  (bases 1 to 2455)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2455)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:10:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,720
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 35
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2455
                     /organism="Petrimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14966_length_2455_cov_2.265417"
                     /isolate="AMR_MDS_1826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2023866"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1058)
                     /locus_tag="PLI30_12905"
     CDS             complement(<1..1058)
                     /locus_tag="PLI30_12905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008780554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HOI80491.1"
                     /translation="MIYPFFKKTTLIATSALLAAALHAQETADTRPNIILFMVDDMGW
                     QDTSLPFGTEKTPLNERYHTPNMERLASQGRMFTQAYACAVSSPTRVSLMTGMNAARH
                     RVTNWTLEKNKSTDRKSDVLKFPEWNVNGICEVPDVPYTYEVTSLPQILKNSGYHTIH
                     CGKAHWGAIDTPGENPHHFGFEVNIAGHAGGGLASYLGEQNFGNRTDGKPSSLQAIPG
                     LKDYWGKDIFATEALTLEAIKALDKARKLRQPFYLYMSHYAIHIPVDKDKRFFQKYRD
                     AGLSEKEAGYASLIEGMDKSLGDLMDWLEKNKMDKNTVVIFMSDNGGLSSEPEWRDTP
                     LHVHNAPLNSGKGSAYEGG"
     gene            complement(1078..2448)
                     /locus_tag="PLI30_12910"
     CDS             complement(1078..2448)
                     /locus_tag="PLI30_12910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008769492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004560 - alpha-L-fucosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-fucosidase"
                     /protein_id="HOI80492.1"
                     /translation="MSLNLLFLFTVFCALPQSHLFPVPTARQLRWAQAEFGVIFHYDL
                     HVFDGKRYDQAINRIHPVEDYNMFNPNELDTDQWIKAAKAAGARFAVITATHETGFAI
                     YQSNVNPYSLKALKWRDGKGDVVGDFVKSCRKYGIQPGVYIGIRWNSLLGIHNFKAEG
                     ESEFARNRQEWYRRFCERMTEELMSKYGDLFMVWYDGGADDPKGLGPDVEPIVNRLQP
                     ECLFYHNTQRADFRWGGSEAGTVGYPCWSTFPNSFSHNKNLDTQRSHVELLKHGDPDG
                     KYWVPAMADAPLRGHNGRHEWFWEPGDEDAVYPLKDLVDMYYNSVGRNATLILGLTPD
                     SRGLVPEGDVQRLKEFGDKIDDLMGHPLKTVAGNGMKLEINLGKRQPVNQVVIQEEIS
                     NGERVRAYVVQGLTVGGWKTLCEGESVGHKRIQTFKSSPVSRIRLLINESIDVPQIKN
                     FSVYCN"
BASE COUNT          606 a          638 c          534 g          677 t
ORIGIN      
        1 ccaccctcgt atgccgaacc ttttccgcta ttgagcggag cgttgtgtac atgtaacggc
       61 gtatcgcgcc attccggttc ggatgagagg ccaccgttat ccgacataaa aattacaact
      121 gtatttttat ccattttgtt tttctcaagc caatccatca aatcacccag gcttttgtcc
      181 atgccctcaa taagtgaagc atatccggct tctttttccg ataatccggc atctctgtat
      241 ttttgaaaga accgcttgtc cttatctacc ggaatatgaa tggcgtaatg agacatgtac
      301 agataaaacg gctgtctcag ttttctagct ttgtccaatg cttttatagc ctctagggtt
      361 aaagcttctg tagcgaaaat atcttttccc caatagtctt ttagacccgg gatggcttgc
      421 aacgatgacg gttttccatc ggtgcggttg ccgaaattct gttcacccag gtaactggct
      481 aatccgccgc cggcatgtcc ggcaatgttt acttcaaaac caaaatggtg cggattttca
      541 cccggagtat caatggcccc ccaatgcgcc ttaccgcaat gaatggtatg ataaccgctg
      601 tttttaagaa tttgtggtaa cgaagttact tcatacgtat acggaacatc aggcacctca
      661 caaatgccgt tcacattcca ttcgggaaat ttcagcacgt cgcttttccg gtcggttgat
      721 ttatttttct ccaatgtcca gttggtaacc cggtggcgcg cggcattcat acctgtcatc
      781 aggctcacac gtgtaggaga gcttacggca caggcatacg cctgcgtgaa catccttcct
      841 tgcgatgcca gtcgctccat gttgggggta tgataacgct cattaagcgg tgttttttct
      901 gttccaaacg gaagtgacgt gtcttgccag cccatatcat ccaccataaa aagaataatg
      961 ttgggacgtg tgtcagcagt ttcttgagca tgcaatgcgg ctgccaacaa ggcagaggtt
     1021 gcaatcaggg ttgttttttt gaaaaatggg tatatcatca cgttatcttt ttgagtatca
     1081 gttacagtac acactaaaat tctttatttg gggcacatca atactttcgt ttatcaacaa
     1141 gcgaatgcgg ctcaccggag aggacttgaa agtctgaatg cgtttatgac caacagattc
     1201 cccttcgcaa agcgtcttcc acccgccgac agtcagtccc tgcacgacat atgcccggac
     1261 acgttcaccg ttgcttattt cttcctggat gaccacctga ttaacaggct gccgcttccc
     1321 aagattaatc tccagtttca ttccatttcc tgcaacagtt tttaaaggat gtcccatcag
     1381 gtcatcaatt ttatcaccaa actcttttag gcgctgaaca tcgccttcgg gcaccagtcc
     1441 ccgtgaatcg ggtgtaagac ccaggataag tgtcgcattg cgtcctaccg aattataata
     1501 catatctacc aggtctttga gcggataaac ggcatcctca tctcccggct cccaaaacca
     1561 ttcgtggcga ccgttatgtc ctctcagggg tgcgtcagcc atggctggga cccaatattt
     1621 cccgtcggga tctccgtgct tgagcaattc gacatggctg cgctgtgtgt cgaggttttt
     1681 attgtgcgaa aacgaatttg ggaaagtact ccagcacgga taccccactg ttccagcttc
     1741 agaaccgccc cagcgaaagt cggcacgctg cgtattgtga taaaacaagc attccggctg
     1801 caggcggttg acaatcggct ccacatccgg gcccaatcct ttcggatcat cggcaccacc
     1861 gtcataccac accataaaca aatcgccgta tttcgacatt aattcttccg tcattcgttc
     1921 acaaaaccga cggtaccatt cctgacggtt gcgggcaaat tcactctcac cttccgcctt
     1981 gaaattgtga atacctaaca atgagttcca gcggatacct atgtaaactc caggttgaat
     2041 gccgtatttg cggcacgatt tcacgaaatc gcccacgaca tcgccttttc cgtctctcca
     2101 tttcaaggct ttcaagctgt atggatttac gttactctga taaatagcaa atccggtttc
     2161 gtgcgtggca gtgatcacgg caaatctagc gcctgcagct ttagccgcct ttatccactg
     2221 atccgtatcc aattcattag gattgaacat gttataatcc tccacggggt ggatacggtt
     2281 gatagcctga tcgtaccttt tgccgtcgaa aacgtgtaaa tcgtagtgaa aaatcacccc
     2341 aaactcagct tgcgcccaac ggagttgccg ggcagtaggc acagggaaca ggtgtgattg
     2401 cggcaatgca caaaacactg taaacaaaaa gaggagattg agggacatcg atgtc
//
LOCUS       DAOSDR010000432         2430 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrimonas sp. isolate AMR_MDS_1826
            NODE_15145_length_2430_cov_4.950316, whole genome shotgun sequence.
ACCESSION   DAOSDR010000432 DAOSDR010000000
VERSION     DAOSDR010000432.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863355
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrimonas sp. (wastewater metagenome)
  ORGANISM  Petrimonas sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Dysgonomonadaceae; Petrimonas.
REFERENCE   1  (bases 1 to 2430)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2430)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:10:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,720
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 35
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2430
                     /organism="Petrimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15145_length_2430_cov_4.950316"
                     /isolate="AMR_MDS_1826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2023866"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..987)
                     /locus_tag="PLI30_12915"
     CDS             complement(<1..987)
                     /locus_tag="PLI30_12915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019539244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1343 domain-containing protein"
                     /protein_id="HOI80493.1"
                     /translation="MRVKKVLFIAALLFFSFNLPAQTVKAGAELTEAYLPLIRGKRVA
                     VMTNQTGRVGDEHLVDLLIRNNVDLVGIFSPEHGFRGTADAGEHVASSVDEETGVPVW
                     SLYGGGGGKPSADKMRTFDVLLFDLQDVGLRFYTYYASMARLMDACAEHNKKMIVLDR
                     PNPNGFYVDGPILDMKHKSGVGWLPIPVVHGMTLGELALMINGEKWLPQGRICDVTVI
                     PCENYTHQTKYELPVAPSPNLPNTQSIYLYPSTCLFEGTVMSLGRGTSFPFQAYGHPN
                     FKGSGFSFTPRSVPGAKNPPLLNKKCYGVDLRNVSHEQIWENRFDLSYVIDAY"
     gene            complement(984..1376)
                     /locus_tag="PLI30_12920"
     CDS             complement(984..1376)
                     /locus_tag="PLI30_12920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019538115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HOI80494.1"
                     /translation="MGKRRKPIQFNDKRLYYSIQEVADHFAVNVSLLRFWEKEFENIN
                     PKKTAGGTRQYTREDIQQVEIVHHLVKEKGLTLEGARQSLKSKKDDEVKRVEALEKLK
                     EIKKDLLSLEEEFDRLHEQQKYSKTEIE"
     gene            1534..>2430
                     /locus_tag="PLI30_12925"
     CDS             1534..>2430
                     /locus_tag="PLI30_12925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase-related protein"
                     /protein_id="HOI80495.1"
                     /translation="MTSKEIRQSFKDFFASKDHQVVPSAPMVIKDDPTLMFTNAGMNQ
                     FKDIILGNAPVKYPRVADSQKCLRVSGKHNDLEEVGHDTYHHTMFEMLGNWSFGDYFK
                     KEAIAWAWEYLVGVLKLDPDRLYATVFEGSPGENLERDNEAAGYWLQYLPKERIIDGN
                     KKDNFWEMGDTGPCGPCSEIHIDIRSEEERAKVDGLLLVNQSHPQVIEIWNLVFMQYN
                     RKADGSLEPLPAKVIDTGMGFERLTMAVQGKISNYDTDVFQPIIAEIGEITSVTYGKD
                     ARKDIAMRVIADHIRTISFSITD"
BASE COUNT          644 a          605 c          559 g          622 t
ORIGIN      
        1 ataggcatcg ataacgtagg aaagatcgaa gcggttttcc cagatctgtt cgtgcgaaac
       61 attccggagg tcaacaccgt aacatttttt gttgagcaac ggcgggttct tagctcccgg
      121 aacgctgcgg ggagtaaagg aaaaacctga gcctttgaag ttcggatgcc cgtaggcctg
      181 gaatggaaaa gaggtcccgc ggcccaggct catgactgtg ccttcgaaaa ggcatgtgga
      241 aggataaaga taaatggact gggtatttgg caggttggga gaaggggcta cgggtaattc
      301 gtattttgtc tgatgcgtat aattttcaca agggataaca gtcacatcgc aaatccggcc
      361 ttggggcaac catttttcgc cgttaatcat cagggcgagc tcacccagcg tcattccgtg
      421 aaccacgggg atgggcaacc accctacacc cgacttgtgt ttcatatcca aaatcggccc
      481 atccacataa aatccatttg ggttgggacg gtcgagtacg atcattttct tattgtgttc
      541 agcacaggca tccattaaac gagccatact ggcatagtag gtgtaaaacc gcaatccgac
      601 atcctgaagg tcgaaaagaa gcacatcgaa tgttctcatc ttatcggcag aaggcttacc
      661 gcctccacca ccgtatagcg accacacagg aactcccgtt tcctcatcaa ccgagcttgc
      721 cacgtgctcc cccgcatcgg ccgttccacg aaacccgtgt tcgggggaga aaattccaac
      781 caggtcaaca ttattccgga taagtaaatc caccagatgt tcgtccccta cccttccggt
      841 ttgattggtc ataaccgcca cccgttttcc ccggatgagc gggagatatg cttcggtcaa
      901 ttcggcccct gctttaacgg tttgtgccgg tagattaaag gagaaaaaga gtaatgccgc
      961 aataaaaagt actttcttta ccctcattct atttccgttt tgctgtattt ttgctgctcg
     1021 tgaagccggt caaactcttc ttccagcgag agcagatcct tctttatttc cttaagtttt
     1081 tccaacgctt ccacacgctt aacctcatcg tcttttttcg acttcagcga ctggcgcgca
     1141 ccttccagcg tcaatccctt ctcttttacc aggtgatgca caatctccac ctgttgaatg
     1201 tcctcccggg tgtattggcg tgttcctccg gccgtctttt tcgggttaat attttcgaac
     1261 tctttctccc agaaccttaa tagcgagaca tttacagcaa aatgatccgc cacctcctga
     1321 atggaatagt aaagccgttt atcgttgaac tggattggtt tccttcgctt gcccatttct
     1381 ttaccaaagt tttgaacctt gacaaagata caaattagaa tcaaaagaca atgatttttg
     1441 ctcatgtttt gacgcaagaa aattgtatct ttgcacctca aaaacaaaac ttcgaatttc
     1501 ccgctcgaaa ttcaaaaaac tcgaaacccg tatatgactt caaaagaaat tcgccaatcg
     1561 tttaaagatt tttttgcatc caaggaccac caggttgtgc cttcggctcc gatggtgatt
     1621 aaggacgacc ctacgctgat gtttaccaat gccggcatga atcagtttaa agatattatt
     1681 ctgggtaacg cccctgttaa atatccgcgg gtggcagatt ctcaaaaatg tttgcgcgtg
     1741 agcggcaagc acaacgatct ggaagaggta ggacacgaca cttatcacca cacgatgttt
     1801 gaaatgctgg gaaactggtc gttcggcgat tatttcaaaa aagaagccat cgcctgggca
     1861 tgggaatatc ttgtgggggt actgaaactc gatccggacc ggctctacgc aaccgttttt
     1921 gaaggaagcc ccggagagaa tctggagcgc gacaatgagg ccgccggtta ctggctgcaa
     1981 tatctgccta aagaacgtat cattgacgga aacaaaaaag ataacttctg ggaaatgggc
     2041 gatacagggc cttgcgggcc ttgttccgaa attcatatcg atatccgatc cgaggaggag
     2101 cgagccaagg tagacgggct tctgctcgtg aaccaaagcc accctcaagt tattgagatc
     2161 tggaacctcg tgttcatgca atacaaccgc aaggccgatg gttcgctgga accgcttcct
     2221 gccaaagtga tcgacaccgg catggggttt gagcgtctta caatggcagt acaaggaaaa
     2281 atatcgaact acgacaccga tgtttttcag ccaatcatcg ctgaaatcgg agaaatcaca
     2341 agtgtgacgt atggaaaaga tgcccggaaa gacatcgcca tgcgtgttat tgccgaccac
     2401 attcgcacca tttctttctc gataaccgac
//
LOCUS       DAOSDR010000433         2408 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrimonas sp. isolate AMR_MDS_1826
            NODE_15325_length_2408_cov_3.917977, whole genome shotgun sequence.
ACCESSION   DAOSDR010000433 DAOSDR010000000
VERSION     DAOSDR010000433.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863355
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrimonas sp. (wastewater metagenome)
  ORGANISM  Petrimonas sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Dysgonomonadaceae; Petrimonas.
REFERENCE   1  (bases 1 to 2408)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2408)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:10:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,720
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 35
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2408
                     /organism="Petrimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15325_length_2408_cov_3.917977"
                     /isolate="AMR_MDS_1826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2023866"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..208
                     /locus_tag="PLI30_12930"
     CDS             <1..208
                     /locus_tag="PLI30_12930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007566039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="magnesium chelatase"
                     /protein_id="HOI80496.1"
                     /translation="LEYAAPDAAGLQLLRTAMDRLSLSARAYDRILKVSRTIADLEAS
                     PHVLPVHLAEAINYRNLDREVWVG"
     gene            238..804
                     /locus_tag="PLI30_12935"
     CDS             238..804
                     /locus_tag="PLI30_12935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010802635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma-70 factor"
                     /protein_id="HOI80497.1"
                     /translation="MKEKDTIYENLFRKVAFRDDEQAFRELFLEFYPALCVFAMRYIT
                     QEETARDVVQDVFFKIWKNRKSMDITVSFRNFLITSVRNHCTDYLRKQEVENRYQEEN
                     MLSVHTSPEEVYTLKELETTIGEALAKLPPNVREAFEMSRFKGMTYIAIADKMEVSPK
                     TIESYISRALKILRIELRDYLPFLLLFI"
     gene            918..1835
                     /locus_tag="PLI30_12940"
     CDS             918..1835
                     /locus_tag="PLI30_12940"
                     /inference="COORDINATES: protein motif:HMM:NF016648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4974 domain-containing protein"
                     /protein_id="HOI80498.1"
                     /translation="MIEKENPDSLNALLENYEKQKTIDAVADWEQLRKRFTSDRNRRL
                     FFNYLRNTAAILLPLFLVYQYTLYPILKKSGSIVETITVTSAPGMVTKTILPDGSEVW
                     LNALSSLTYPQRFTEKERRVQLSGEAYFKVTSDKKHRFNVETPQKMVVSAYGTEFNVN
                     AYENEASHEVTLASGQVEISSEIGSKVTETLVVDEKAILQVKTGNIHVVTADTYVETA
                     WKDGKMVFRREKLDKIVTRLSRKFGVDIHLEGDRLKEYEYTATFTDETLEDILDLLKR
                     SAPITYSISKQKQLNNETFTRREVIIKSR"
     gene            2071..>2408
                     /locus_tag="PLI30_12945"
     CDS             2071..>2408
                     /locus_tag="PLI30_12945"
                     /inference="COORDINATES: protein motif:HMM:NF019283.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="STN domain-containing protein"
                     /protein_id="HOI80499.1"
                     /translation="MRITLLLLLVLTFQLQAEHIYSQDTKISLNLKHSTVEKVLQTIE
                     EKSDYYFLYNSQLIDVDRKVSVRVRNATISAVLERLFKSENVDYEVKGTHIILSPKEM
                     YSQIATVIET"
BASE COUNT          788 a          516 c          503 g          601 t
ORIGIN      
        1 gctcgaatat gctgctcccg atgcagcggg actgcaatta ctcagaactg ccatggacag
       61 gcttagccta tcggcaaggg cctacgacag gatactgaaa gtttcccgga ccattgccga
      121 tctggaagcc agcccccatg tcttaccggt tcatctggcg gaagcaatca actaccgcaa
      181 tctcgacagg gaggtttggg tggggtgatt tgaaacggaa agaagtaagg agaaagaatg
      241 aaagaaaaag atacaatata tgagaacctg tttcgaaaag ttgcttttcg ggacgatgag
      301 caggccttca gggaactttt tctcgaattt tatccagcac tgtgtgtgtt tgccatgcgg
      361 tatatcacac aggaagaaac agccagagac gttgtgcagg atgttttttt taaaatttgg
      421 aaaaaccgga aaagtatgga tataaccgtt tcttttcgca attttctgat tacttccgtc
      481 cgcaaccatt gtacggacta tctacggaag caggaagtag aaaaccgcta tcaagaagaa
      541 aacatgttgt ccgtccacac ttcaccggaa gaagtgtata ccttaaaaga gttggaaacc
      601 actattggcg aagcattggc aaaactaccg cccaacgtgc gtgaagcgtt cgagatgagc
      661 cgctttaagg gaatgaccta cattgctata gccgataaaa tggaggtttc cccaaaaacc
      721 attgaatcct atatcagtag ggcattaaaa attttacgca ttgaattgcg tgattacctt
      781 ccctttttac tgctgtttat atagatattc cttttcaccc cacattgtta aatttttact
      841 tttcttaaaa aacttcactt tttttatagg gtaacccggg agttgttcgt caagtatata
      901 taaatgtcaa aaacgagatg attgaaaagg agaatcctga ctctttgaat gctttactgg
      961 agaactacga gaaacaaaaa acgatagacg ctgtagctga ttgggaacaa ttgcggaaac
     1021 gcttcacgtc cgatcggaac aggcgcttgt tcttcaacta cctgagaaat acggccgcga
     1081 ttcttcttcc cctgtttctg gtataccagt atacgctcta tcccattctc aaaaaaagcg
     1141 ggtccatcgt agaaaccatt acggtcacct ctgctccggg gatggtgaca aaaaccatac
     1201 ttccggacgg aagcgaagta tggctgaatg cgttaagctc acttacctat ccacaacgct
     1261 tcacagaaaa agaacgcagg gttcaacttt ctggagaagc ttatttcaaa gtaacctccg
     1321 acaagaaaca ccgttttaat gtggaaacac cccaaaaaat ggtcgtcagc gcttacggaa
     1381 cggaatttaa cgtaaatgca tatgaaaatg aagcaagcca cgaggttact ttggcaagcg
     1441 gacaggtaga gatctcttct gaaatcggct caaaagtaac ggaaacgttg gtggttgacg
     1501 aaaaggcgat cctccaggtt aaaaccggta atatccatgt ggtaacggca gatacctacg
     1561 tagaaacagc ctggaaagac ggtaaaatgg tgttccgccg ggaaaaattg gataagattg
     1621 tgaccaggct ttcgcgcaaa ttcggagtag atatacacct tgagggcgac aggcttaaag
     1681 agtatgaata cacggccacg tttaccgacg aaacgctcga ggacatcctc gacctattga
     1741 aacggtcggc ccccattacc tattccattt ctaaacaaaa acaattaaac aatgaaacat
     1801 tcactcgaag agaagtaatc ataaaatcga gataaggagg accgcctatg acaagataaa
     1861 taccaattga aatacaaaaa aaacgggaga gtaaataccc ctcccgccca tgatttacat
     1921 tgccatcgcg gaaaatctca cctcaccgcc aggcaatcaa ttactaaacc cataaaatga
     1981 atttaataac aatgtaaaag tatgaataat aaatcaatca accaaaaagc acaccttttt
     2041 tatgctttat taaaaaaaat ccctattgct atgagaataa cgcttttact actgctcgtg
     2101 ctcacttttc aactgcaagc tgaacacatc tattctcaag acaccaaaat ttcgctgaat
     2161 ttgaagcatt caaccgtaga gaaagtattg caaaccattg aggagaaatc cgattattac
     2221 tttctgtaca acagccagtt aatcgatgta gaccggaaag taagtgtgcg ggtaagaaat
     2281 gccactattt ccgctgtact ggagaggctt tttaaatctg aaaatgtgga ctatgaagtg
     2341 aaagggacac atatcatcct ttctcccaaa gagatgtaca gccagatagc cactgttatc
     2401 gaaacatt
//
LOCUS       DAOSDR010000434         2400 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrimonas sp. isolate AMR_MDS_1826
            NODE_15405_length_2400_cov_2.377399, whole genome shotgun sequence.
ACCESSION   DAOSDR010000434 DAOSDR010000000
VERSION     DAOSDR010000434.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863355
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrimonas sp. (wastewater metagenome)
  ORGANISM  Petrimonas sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Dysgonomonadaceae; Petrimonas.
REFERENCE   1  (bases 1 to 2400)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2400)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:10:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,720
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 35
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2400
                     /organism="Petrimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15405_length_2400_cov_2.377399"
                     /isolate="AMR_MDS_1826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2023866"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2397)
                     /locus_tag="PLI30_12950"
     CDS             complement(<1..2397)
                     /locus_tag="PLI30_12950"
                     /inference="COORDINATES: protein motif:HMM:TIGR04056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0071702 - organic substance transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SusC/RagA family TonB-linked outer membrane
                     protein"
                     /protein_id="HOI80500.1"
                     /translation="MGANIIEQSTTNGTVTDIDGKFFLNVGVNAIIHVSYIGYLPQDI
                     NTSGKTIISIVLIEETQSLEEVVVVGYGSQKKVNLTGAVAVVDGQAIASKNSTDVVNA
                     MMGQIPGLLVTRNGGEPGAEISGMRIRGFTSVNNAQALVLIDGVEGSLQMLNAEDIES
                     ISVLKDAASASIYGSRAAAGVILVTTKKGSVQKPEISYSGSFGLNVPGVMAQRIPPWE
                     EYEWIHQSLHNVIPTFVINREAIDFIANPNVNYRSIGGLWDEFYSNNWVHLGMKENTL
                     QQNHSVSITGGNDRSRYYVSGGLYTKSGILRYGPDSNNRTNFRVSLNTEINKYLDFNM
                     QASYDGNLRKQIAYPDGAIGMLIFLYNKPGYHGLFVPEEDTYRDIDPYSRGGFGGNPI
                     QIMKEGGKIRNQIFSLTALCSFRIKNVVKDLTLDLNFSRRSIFDVTEGDYNFIKGIGR
                     NGTPQPFMDINNPQRVIKTKANSYQDKVEALLNYDWKLSEHHVHILGGASYEQYLRDY
                     ISAEARNGISNDFFSFNYYDNSVASNSRLNDSVEPWKMASMFGRVNYDFAGRYLFEAN
                     ARFDGSSRLSPGNRWEMFPSASVGWRISEEPFFEEAKKVVSNLKIRASYGNLGNSTAF
                     SNDLYPYLDMISRTIFWSMPVYYTNALVSKNLKWETITSTNVGVDMGFLDNRLNFTGD
                     YYWKKNNDMLARLKTGNIIGVGSLPLENVGLLKTWGWELSLQWRDRIGDLSYHATFQI
                     DDSQNKLVKYTGNRVINEGTVQFLEGYPINTIWGYQTDGFWSSREEYLAYKEANPGYE
                     T"
BASE COUNT          664 a          563 c          452 g          721 t
ORIGIN      
        1 tgtttcatac cccggattgg cttccttgta agctagatat tcttcacgac tactccagaa
       61 accgtccgtc tgatatcccc atatcgtgtt aataggatat ccttcaagaa actgtaccgt
      121 gccttcattt atcactctgt tgccagtgta tttgactaac ttgttctgac tgtcgtctat
      181 ttggaaagtt gcgtgatagg aaaggtcgcc gatgcggtca cgccattgca atgacagttc
      241 ccaaccccat gttttcaaca agccaacatt ttccaaggga agagagccaa cgccgataat
      301 atttccggtt ttcagccgcg ccagcatatc attattcttt ttccagtagt aatcaccggt
      361 aaaattcaac cggttatcca gaaaacccat atccacacct acatttgttg aagtaatggt
      421 ttcccatttc agatttttcg agaccagtgc attcgtgtaa taaaccggca tactccagaa
      481 tatggtcctt gaaatcatat ccaaataagg ataaaggtcg ttgctaaacg cggtactgtt
      541 tcctaaattt ccatatgaag cacgtatctt aagattgctg acaacctttt tggcttcttc
      601 aaaaaacggt tcttcactga tgcgccaccc tacagaagct gaaggaaaca tttcccaacg
      661 gttaccagga gacaaccgtg agctgccatc gaagcgggca ttggcttcga aaagatagcg
      721 tcccgcaaaa tcgtaattta cgcgtccaaa cattgaagcc attttccacg gctctacaga
      781 atcattcaac ctagaattgg atgctacact gttgtcatag taattgaaac tgaagaaatc
      841 attcgaaatc ccattccgtg cttcggcaga aatatagtcc cgaagatact gttcgtacga
      901 agctccaccc aatatatgaa catggtgttc gcttaatttc caatcgtaat tcaataatgc
      961 ttccactttg tcctgatagg aattggcttt tgttttgatt accctttgtg ggttgttgat
     1021 atccataaaa ggttgaggtg taccatttct gccgatgcct ttaatgaaat tataatctcc
     1081 ttctgttacg tcaaagattg accttcttga aaaattaaga tcgagcgtca ggtctttcac
     1141 cacattcttg atacgaaaac tacaaagcgc cgttaaagag aaaatttgat ttcttatctt
     1201 tccaccctct ttcatgattt ggatggggtt gccgccaaaa ccacccctgg aatacgggtc
     1261 aatgtcgcga taggtgtcct cttcggggac aaaaagccca tgatatccgg gtttgttata
     1321 caaaaagatc aacataccta tagctccatc aggatacgca atttgtttcc tcaggtttcc
     1381 gtcgtatgaa gcttgcatat tgaaatccag gtatttgttg atttcagtat tcaacgaaac
     1441 tcggaaattg gtacggttgt tactgtcggg tccgtatcgg agaattccac ttttggtata
     1501 caaaccgcct gatacataat aacgtgatct gtcgtttcca ccggttatcg atactgaatg
     1561 gttctgctga agtgtattct ctttcatccc caaatgcacc cagttattgg agtaaaattc
     1621 atcccacaac cctcctatag aacgataatt tacattggga ttagctatga agtcaatggc
     1681 ctccctgttt ataacaaagg tggggataac gttatgcaag gattgatgga tccactcgta
     1741 ttcttcccat ggcggaatac gttgtgccat cacccccggc acattaagcc caaacgaacc
     1801 gctgtaggat atctctggct tttgaacaga gccttttttg gtagttacca gaattacgcc
     1861 ggcagcagcg cgcgatccgt agatggaagc cgatgccgca tctttcagta ctgaaatgga
     1921 ttcaatatct tcagcattta acatttgtaa cgatccttcc actccatcaa tcagaacgag
     1981 ggcctgggca ttgttcacag acgtaaaacc gcgtatacgc ataccgctta tttccgcacc
     2041 gggttctccg ccattacgcg taacaagtaa tcccggtatc tgtcccatca ttgcgttcac
     2101 cacgtcggta gagttcttag aagcaatagc ctgtccatca acaacagcaa cggcaccggt
     2161 tagatttacc tttttttgtg aaccgtatcc tactacgacc acctcttcaa gtgattgtgt
     2221 ctcttcaatt aaaactatgc ttatgatggt tttaccagaa gtatttatat cttgtggaag
     2281 ataaccgata taagatacat ggattattgc attcactcca acattcaaaa aaaactttcc
     2341 atcaatatct gtcactgtac cattagttgt actttgttca ataatatttg ctccaattat
//
LOCUS       DAOSDR010000435         2372 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrimonas sp. isolate AMR_MDS_1826
            NODE_15629_length_2372_cov_4.347432, whole genome shotgun sequence.
ACCESSION   DAOSDR010000435 DAOSDR010000000
VERSION     DAOSDR010000435.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863355
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrimonas sp. (wastewater metagenome)
  ORGANISM  Petrimonas sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Dysgonomonadaceae; Petrimonas.
REFERENCE   1  (bases 1 to 2372)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2372)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:10:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,720
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 35
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2372
                     /organism="Petrimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15629_length_2372_cov_4.347432"
                     /isolate="AMR_MDS_1826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2023866"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..734
                     /locus_tag="PLI30_12955"
     CDS             <1..734
                     /locus_tag="PLI30_12955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019538522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HOI80501.1"
                     /translation="QAEENNGGILLITEGVFGMSGDLGNLPGIVALKKQFNFRLMIDD
                     AHGFGTMGKTGAGASEHFGLMNEVDIYFATFAKSMAGIGAFVASDKAVVDSLKYNMRS
                     QIFAKSLPMPMVIGALKRLDILQNRPEIRENLWKIAKALQDGLVAEGFNIGNTQSPVT
                     PVYFSGSVPEGTNIVIDLRENHNVFCSIVVYPVVPKGVLMLRLIPTASHTMKEVNETI
                     DVFKKIKVNLEAGKYISDEMRSMTM"
     gene            740..2071
                     /locus_tag="PLI30_12960"
     CDS             740..2071
                     /locus_tag="PLI30_12960"
                     /inference="COORDINATES: protein motif:HMM:NF012512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HOI80502.1"
                     /translation="MRLRVKRQKKYYLKCAGCGFVTPSFKAWFDQFQKCPNCGSKHSE
                     VWYNTSYKRLPRFIKGNPQNFWHYFPYLPLVLKRHIITRYEGTVPIERWHFLEEFAKK
                     EYGLTLEVHAYRNDLNGGTGTFKDVAAALAASIFKENNLKQYCIASTGNSATAYAKYL
                     SIAGVNCSVFMPEDAIRASEATISSFGQQVFRVMGDYAKAKEVAAEYARRHHIQMSTG
                     NIDPIRIEAKKTMVFEWLRQLGKAPDVYIQAISGGTGPLAIDKGIREIGHLYPDLQNP
                     RFIMVQSDGCDPMVQAWEKAEAEGFPEGFEKNYPVIDNPKTNVPTLATGNPATYPIIS
                     KLVRKTGGSFVRMRESKLIPLGKLIAYEQKMVPGPASTVCVAGFFNALKKNLINDGET
                     VMVNIGEGAVRAPYFLEQMIYTTQEVTNADECAPHEIDGYRDQLWKDVLND"
     gene            2124..>2372
                     /locus_tag="PLI30_12965"
     CDS             2124..>2372
                     /locus_tag="PLI30_12965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019538524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HOI80503.1"
                     /translation="MQKSTNKIIWITGASSGIGEACAYIFAAEGNKLILTGTREGQLK
                     KVQQKCIDLGAECEILPADLSQTEQLDTLTDRALSFFGK"
BASE COUNT          684 a          546 c          576 g          566 t
ORIGIN      
        1 aacaggccga agaaaataac ggtggaatct tgttaatcac cgaaggggtt ttcggaatgt
       61 caggtgatct gggcaacctg ccgggaattg tagcgttgaa aaaacaattc aacttccggt
      121 taatgatcga tgacgcacac ggattcggaa cgatgggaaa aacgggagca ggagcgagtg
      181 aacatttcgg attgatgaac gaagtggata tttacttcgc cacatttgcc aaatcgatgg
      241 caggtattgg ggcttttgta gcctcggata aagccgttgt cgattcgctg aagtacaaca
      301 tgcgttccca gattttcgcc aaatctctcc cgatgcctat ggtaatcggc gcgctcaaac
      361 ggttggatat tctccaaaat cgacccgaaa taagagaaaa tctatggaaa atagccaaag
      421 cactccagga tggccttgtg gccgaagggt ttaacatcgg gaatacgcaa tccccggtca
      481 cccctgttta tttcagtgga tcggtacccg aagggacaaa tattgtgatt gatctgcgtg
      541 aaaaccacaa tgttttctgc tccatcgttg tctatccggt agtgccgaag ggtgttttaa
      601 tgctccgcct tatacctacg gcatcacaca cgatgaagga agtaaacgaa accatagacg
      661 ttttcaaaaa gataaaagtc aacctcgaag caggaaaata tatttctgac gaaatgcggt
      721 cgatgaccat gtaatagaaa tgagactacg cgtaaaaagg caaaaaaagt actacctgaa
      781 atgtgccgga tgcggttttg tgacacccag tttcaaagca tggttcgatc agtttcagaa
      841 gtgccccaat tgcggcagta agcactccga agtttggtat aacacctctt acaaacgatt
      901 accccgtttt atcaaaggga atccgcaaaa tttctggcac tactttcctt atttgccgtt
      961 ggtgctaaaa aggcacatca ttacccggta cgaagggacc gttcctatcg aacggtggca
     1021 ttttttggaa gagtttgcca aaaaagaata cgggttaacc cttgaggtcc acgcttaccg
     1081 caacgatttg aacgggggaa caggtacatt caaagatgtt gccgcagcac ttgcagcgag
     1141 catattcaaa gaaaataacc tgaaacaata ctgcattgct tccaccggaa actcggctac
     1201 cgcatacgct aagtacctgt ccattgccgg agtgaactgt tcggttttta tgccagaaga
     1261 cgctatccgg gcctcggagg ccaccatcag ttctttcggg caacaggtct tccgcgtaat
     1321 gggtgattat gccaaggcca aggaagtggc cgccgaatac gcccggcgtc accacataca
     1381 gatgtccacc gggaacatcg atcccatccg gatcgaagcc aaaaagacaa tggtttttga
     1441 atggttgcgt caactcggga aagcacccga tgtatatatt caggccatca gtggaggtac
     1501 aggtccatta gcgattgata aaggaatacg tgagatcggt cacctctatc ctgatcttca
     1561 gaatccacgc tttatcatgg tgcaatccga cggttgcgac ccgatggttc aagcgtggga
     1621 aaaagcagaa gccgaaggct ttcctgaagg tttcgagaaa aattatcccg tcatcgacaa
     1681 tccaaaaacc aatgttccca cgctggcgac cggaaatccg gctacctacc ccatcatttc
     1741 gaaattagtc cggaaaaccg gcggttcatt cgtcaggatg cgcgaatcga agctgattcc
     1801 gctggggaaa ctgatcgctt acgagcagaa aatggttccg gggcctgcat caactgtttg
     1861 tgtggccggc ttttttaatg cattgaaaaa aaacctgatc aacgacggag agacggtaat
     1921 ggttaacatc ggggaagggg ctgttcgtgc accctatttc ctggagcaga tgatctacac
     1981 cacacaggaa gtcaccaatg ccgatgagtg tgcaccccac gaaatcgacg gttaccggga
     2041 tcagctttgg aaagatgtat taaatgatta attttcacaa taacctgact atctagtgtc
     2101 tttctctcgt tgttcctaaa actatgcaaa aaagtacaaa taaaattata tggatcactg
     2161 gcgcttcgtc gggtatcggc gaagcctgcg cgtatatatt cgcagctgaa gggaacaagt
     2221 tgattctgac aggcacccgt gagggccaat tgaaaaaagt acagcaaaag tgcatcgatt
     2281 tgggtgcgga gtgtgagatt ttgcctgccg acctatccca aacagaacaa ttggataccc
     2341 ttaccgaccg ggcactgtcc ttctttggca aa
//
LOCUS       DAOSDR010000436         2359 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrimonas sp. isolate AMR_MDS_1826
            NODE_15727_length_2359_cov_2.582899, whole genome shotgun sequence.
ACCESSION   DAOSDR010000436 DAOSDR010000000
VERSION     DAOSDR010000436.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863355
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrimonas sp. (wastewater metagenome)
  ORGANISM  Petrimonas sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Dysgonomonadaceae; Petrimonas.
REFERENCE   1  (bases 1 to 2359)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2359)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:10:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,720
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 35
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2359
                     /organism="Petrimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15727_length_2359_cov_2.582899"
                     /isolate="AMR_MDS_1826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2023866"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1913)
                     /locus_tag="PLI30_12970"
     CDS             complement(<1..1913)
                     /locus_tag="PLI30_12970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007652628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0071702 - organic substance transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SusC/RagA family TonB-linked outer membrane
                     protein"
                     /protein_id="HOI80504.1"
                     /translation="MKKIALLLLVIAVIGVQALTAQVKRISGTVRDAGDNSPIPGVSV
                     VCKGTSIGTITNTNGAYQLDVPNNATALVFSFVGMKTQEIPITGTQIDVKMGAEVIGV
                     DEVLVVAYGTAKKESFTGSAEVISPDKLQKRTIANVSKALDGMVAGVQTTSGSGQPGS
                     GSSIVIRGFGSIQASNNPLYVVDGIPYDGNISAINPNDIESLTILKDASAGALYGARG
                     ANGVILITTKRGQKGALSVDLKANWGISSRAVPRYEVMDEKGYLETIYQSYRNEQITN
                     GISPDKAGLAALAAMKTGAKKIFGDNEMYNPFNYTITELIDPVTGKVRSDATLKYSED
                     WLDEVTAQNPLRQEYILGLSGSNDKTRYLFSLGFLDEEGLLKTTNFKRYTGRINVDSE
                     PKAWMKMGLSSNFSLNTSNTSNTSTTATSNVFYSAQLMAPIFPVYLRDAEGNVVLDAL
                     GKKVFDYGLSRPSGASGGFNSVATLYEDKYSSNSDNVSARTFLALGDLKNGPLQGLKL
                     SVNLGFDYVDSYSSTYYNPYFGNSATVKGMISKTNARTLGYTLNELLTWTRAFGQHHV
                     DVLAGHEYYDYKYNTLGGTKTGFPFGGLYELDAATTITDATSYEDTYRIESILTRANY
                     DFANKYYLSASFRT"
     gene            2210..>2359
                     /locus_tag="PLI30_12975"
     CDS             2210..>2359
                     /locus_tag="PLI30_12975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004329929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HOI80505.1"
                     /translation="MNFIEEIKRRRTFGIVSHPDAGKTTLTEKLLLFGGAIRVAGAVK
                     SNKIKK"
BASE COUNT          625 a          568 c          536 g          630 t
ORIGIN      
        1 gtcctgaagc tggcagaaag gtaatatttg ttggcaaagt cgtagttggc acgggtcagt
       61 atcgactcga tccggtaggt atcttcgtaa gatgtagcat cggtaatcgt agtggcagca
      121 tccaattcat agagtccgcc aaacgggaaa cctgttttgg ttcctcccag cgtattgtac
      181 ttgtaatcgt agtactcatg accggccagc acgtcgacgt gatgttgtcc aaatgcccgg
      241 gtccaggtaa gcagttcgtt taaagtgtac cccagggtgc gggcgttggt tttggagatc
      301 attcccttta cggtagccga gttaccgaag taagggttat aataggtcga agaatagctg
      361 tctacgtagt cgaaacctaa gttaacgctc agtttcaaac cttgcagcgg accatttttc
      421 aggtcaccga gtgccaggaa agttcttgca ctgacgttat cgctgttgga gctgtatttg
      481 tcttcataca acgtagccac actgttgaac ccaccactgg cgcccgaagg acggctcagt
      541 ccgtaatcaa acactttttt gcccagggcg tccaacacaa cgttgccctc agcatcgcgg
      601 agataaaccg ggaagatcgg cgccatcagc tgagctgaat agaagacgtt ggaagttgcc
      661 gtggtgctgg tattggaggt attgctggta ttcagcgaga agttggagct caaccccatt
      721 ttcatccacg ctttcggctc agaatctaca ttgatcctac cggtgtaacg tttgaagttg
      781 gttgttttga gcaacccttc ttcatccagg aaacccaacg agaagaggta gcgggttttg
      841 tcatttgagc cggaaagccc aaggatatat tcctgacgta acggattttg agcggtaacc
      901 tcatcaagcc agtcttcgct gtatttcagc gtggcatccg aacgaacctt tccggtaacc
      961 ggatcaatca actccgtaat agtgtagttg aaagggttgt acatctcatt atccccaaat
     1021 atttttttgg cacctgtttt catcgccgcc aatgcagcaa ggccagcctt gtcaggagaa
     1081 attccgtttg taatctgctc gttgcgatag gattgataaa tggtttccaa atatcctttt
     1141 tcatccatca cttcgtagcg tggcaccgcc cgcgaagaga ttccccagtt ggctttcaga
     1201 tcaaccgaaa gggctccttt ttgacctctt ttggtggtga tcaggatcac cccgttggct
     1261 cccctggctc cgtaaagagc gccggcagaa gcatctttca aaattgttaa cgactcaatg
     1321 tcgttggggt tgatggccga aatattacca tcatacggaa taccgtcaac cacgtacagt
     1381 ggattgttcg aagcttggat agaaccaaat cccctgatca cgatggaaga gcccgatccg
     1441 ggttggccgc tacccgaagt ggtctgaaca ccggccacca taccgtctaa cgctttcgaa
     1501 acgttggcaa tggttctttt ctgcagttta tctggtgaga taacttcagc cgaaccggta
     1561 aatgattctt ttttggcggt tccgtaagca accaccaata cttcatccac accgattacc
     1621 tcggcgccca tcttcacatc gatttgtgta ccggtgatcg gaatttcctg ggttttcatc
     1681 cccacaaatg aaaaaacaag tgccgtggca ttgttcggca catccaactg gtaagctcca
     1741 ttggtattgg taatggtccc tatgctggtt cccttacaca ccactgaaac ccctgggatc
     1801 ggcgaattat cccctgcatc tctgacagtt ccggatatac gcttaacctg ggcagttaaa
     1861 gcctggacgc caattacagc aatcacaagc agcaatagcg caattttttt catagactaa
     1921 attttagatt aataaattaa aacagacgct tattctcctg ctgcgaatgt aagaaaaaaa
     1981 acaaaaaatt aacttctttt aagatcctgc ctgcattttt tgggaaaatg tcagtgtttt
     2041 ttaacaattc acaagaatta cccagatcta gcttcgaaac tcaatattga caaaaacagg
     2101 atttcagttg ccaataatac agttacacaa tctcttccat aatctctgag cggaaatctt
     2161 tcgatactaa aaatttctaa ttttgcggct tctgaaaaga aaagcaagca tgaattttat
     2221 cgaggagatt aaacgcaggc gcaccttcgg gattgtgagc catcccgacg ccggaaaaac
     2281 cacgctcaca gagaaactgc tgctctttgg cggcgccatc cgggtagccg gagcagtaaa
     2341 aagcaacaag atcaaaaag
//
LOCUS       DAOSDR010000437         2344 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrimonas sp. isolate AMR_MDS_1826
            NODE_15842_length_2344_cov_2.599388, whole genome shotgun sequence.
ACCESSION   DAOSDR010000437 DAOSDR010000000
VERSION     DAOSDR010000437.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863355
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrimonas sp. (wastewater metagenome)
  ORGANISM  Petrimonas sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Dysgonomonadaceae; Petrimonas.
REFERENCE   1  (bases 1 to 2344)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2344)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:10:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,720
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 35
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2344
                     /organism="Petrimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15842_length_2344_cov_2.599388"
                     /isolate="AMR_MDS_1826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2023866"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(48..923)
                     /gene="pstA"
                     /locus_tag="PLI30_12980"
     CDS             complement(48..923)
                     /gene="pstA"
                     /locus_tag="PLI30_12980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013612219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HOI80506.1"
                     /translation="MMERKQTSIRYRSSKDKLVFVLVCALSALSMLPLFFILWEVISR
                     GYKNFNLQLFSEVTPSSMDAMLSRINGESIPGGILNGITGTLLIVGVAILLTIPLGLL
                     IGIYLSENPQKKFARLASYLTDLLQGMPSIVIGIIAYAWVVKPLGGYSALAGSVALTI
                     MMLPMVVRSTEETLKMLPRSYKEAGLALGSSYRNVVFKILLPSGFGGIFTGILLAVSR
                     VMGETAPLMLTALGATVVNWNLMEPTSAIPLLIWDFYNDPNLVDLIWSTSLLLLIVIF
                     LLNWLAKIVARKWRI"
     gene            complement(920..1777)
                     /gene="pstC"
                     /locus_tag="PLI30_12985"
     CDS             complement(920..1777)
                     /gene="pstC"
                     /locus_tag="PLI30_12985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019540741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HOI80507.1"
                     /translation="MKDNLFKAILFTAAASVLLLTAGIIYALVGSASEAFREYGFLGF
                     ITNSEWDPRPATEEYGAFSFVVGTLFTASFALLICLLFSLPVALFTGEYFKGTKISSF
                     VSSVVNLLAGIPSIVYGLWGFYAIRPLVVDLGINSQGFGVFTASVVLAIMIIPYASSL
                     SGEFLSMVPNNLKEGAYSMGATRMEVIQHISLPTAGSGIVAAYILALGRALGETMAVT
                     MLIGNTNHVPKGLADTGNTMASIIANQFGEADGLKLSALIAIGLLLFLITAIINFIAK
                     LVMKYLSRV"
     gene            complement(1774..>2344)
                     /locus_tag="PLI30_12990"
     CDS             complement(1774..>2344)
                     /locus_tag="PLI30_12990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019540740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HOI80508.1"
                     /translation="LCEVSDKWKRSMGKGKALKWSAGIAAKGNPGVAGIVQQTEGAIG
                     YIGSEYALTLKLSTAKLKNKSGNYVDATLETISAAANVDLPDDMRVTLTDSADPNAYP
                     ISLLTWILVYKNQQYANRTEKDARDLVNLLTYVLSPEGQEVAAKINYAPLSEQALIKT
                     QKLISEIHYGGKVLQSANPDPLPWQNVKR"
BASE COUNT          665 a          591 c          486 g          602 t
ORIGIN      
        1 taccgacacc gattttaatt gtaggatagg cttattgccg ttataattta tatcctccat
       61 ttgcgtgcta ctatttttgc cagccagttt aaaagaaata tgacgatgag aagcagcaac
      121 gaagtcgacc agatcaggtc aaccaaatta gggtcgttgt aaaaatccca gatcagaaga
      181 ggaatagcgc ttgtgggctc catcaggttc cagtttacca cggttgctcc cagggccgtc
      241 agcatcaagg gtgcggtctc gcccattacc cgggagacag caagtaaaat tccggtaaaa
      301 attccaccga atcccgaggg aagcaatatc ttgaaaacca cgttcctgta ggaagaacct
      361 aatgccagtc ctgcttcctt ataggaacgg ggcagcattt tcagtgtttc ttcagttgaa
      421 cgcaccacca taggcagcat cattatcgtc agcgccacgc ttcctgccag ggcggaatac
      481 ccgcccaaag gcttcactac ccatgcataa gcgataatcc ctataacgat tgaaggcatt
      541 ccctgcaaga ggtcagtcag gtaactggcc aaccgggcaa actttttttg cgggttctcc
      601 gaaagataaa tcccaatcag caatcccagc ggaattgtca gaagaatagc tactccaaca
      661 atgagcagag ttccggttat tccgttcagg attcctccgg gtatggattc gccgttgata
      721 cgtgatagca tagcatccat cgacgacggt gtcacttcag aaaacagctg taggttgaaa
      781 tttttgtatc ccctggatat tacttcccac aaaatgaaga ataacggcag catcgataag
      841 gccgacaatg cacagacaag aacaaacacc aatttgtcct tgctgcttcg gtaccttatg
      901 cttgtttgtt tcctctccat cataccctcg acaagtattt catgaccagt tttgctataa
      961 aattgataat tgccgtaatt aaaaaaagca acaacccgat agcgatcaac gcacttagtt
     1021 tcaacccgtc agcttcacca aattgattgg caataatgct tgccatcgtg tttcctgtat
     1081 cggccaaccc tttcggaaca tgattggtat tcccgataag catggttacc gccatagtct
     1141 cgcccagtgc tctaccgagc gccaagatat atgcggctac aatcccggag ccggccgttg
     1201 gcaaactgat gtgttgaata acctccattc ttgtcgcacc catactgtaa gcaccttctt
     1261 tcaggttatt gggaaccatc gacaaaaatt ctccgctcag tgaagaggca taaggaataa
     1321 tcatgatcgc cagtacgact gatgccgtaa aaactccgaa tccctgagaa tttatcccta
     1381 aatctaccac aagcgggcgt atggcataaa atccccacaa accataaaca atggaaggaa
     1441 ttccagcaag taaattgaca accgaactca caaacgagga aatcttagtc cctttgaaat
     1501 attctccggt gaacaaagcc acaggcaatg agaacagaag acagatcaat aatgcaaacg
     1561 atgccgtaaa aagtgttccc acaacaaaag aaaacgctcc gtactcttcg gttgccggcc
     1621 tcggatccca ctcggaattt gtaataaaac cgagaaaacc atattctctg aaagcttctg
     1681 aagcactacc caccaaagcg taaattattc cggctgtaag tagcaagacg cttgctgcag
     1741 ccgtaaacaa aatagcttta aacaagttat ctttcatctc ttaacgtttt gccagggtaa
     1801 cggatccgga ttggcagact gcaggacctt gcctccgtaa tggatttccg aaattaactt
     1861 ttgtgtcttt atcaacgcct gctccgacaa gggggcgtaa ttaattttcg ctgcgacttc
     1921 ctgcccttcg ggtgagagca cgtaggtgag caaattgact aaatcccggg catccttttc
     1981 ggttctgttt gcatattgct ggtttttgta caccaaaatc catgtcaaca gactgattgg
     2041 gtaagcattg ggatcggcag aatcggtaag cgtgacccgc atatcatcgg gtaaatccac
     2101 gttagcagca gccgagatag tttcaagtgt ggcatctaca taattaccgg atttattttt
     2161 aagttttgcc gtagacagct ttaatgtgag tgcatattca gaaccgatgt aaccgatagc
     2221 cccttcggtt tgctgaacaa ttcccgccac tccagggttc cctttcgcgg ctattccggc
     2281 actccacttc agggcttttc cttttcccat gctgcgtttc catttatcac tcacctcgca
     2341 taaa
//
LOCUS       DAOSDR010000438         2332 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrimonas sp. isolate AMR_MDS_1826
            NODE_15957_length_2332_cov_4.242424, whole genome shotgun sequence.
ACCESSION   DAOSDR010000438 DAOSDR010000000
VERSION     DAOSDR010000438.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863355
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrimonas sp. (wastewater metagenome)
  ORGANISM  Petrimonas sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Dysgonomonadaceae; Petrimonas.
REFERENCE   1  (bases 1 to 2332)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2332)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:10:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,720
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 35
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2332
                     /organism="Petrimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15957_length_2332_cov_4.242424"
                     /isolate="AMR_MDS_1826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2023866"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            407..994
                     /locus_tag="PLI30_12995"
     CDS             407..994
                     /locus_tag="PLI30_12995"
                     /inference="COORDINATES: protein motif:HMM:NF014689.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese efflux pump MntP family protein"
                     /protein_id="HOI80509.1"
                     /translation="MDYISIMLIAIGLAMDSFAVCVGKGMCRQRFYLWRSLKIAAVFG
                     LFQGLMPFVGYVLGISFASWMQLFDHWFAFFILSVIGIKMIYESFISKPHEVCVGCDC
                     QEDIVVDWRKVVVLAFATSIDAMATGLVFVSYPGTISTATITIGLVTLFFAFSGMFIG
                     VRFGRRLRLNIEMLGGIILIAIGMKILFQHLLHGA"
     gene            complement(1016..1375)
                     /locus_tag="PLI30_13000"
     CDS             complement(1016..1375)
                     /locus_tag="PLI30_13000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004361323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 family protein"
                     /protein_id="HOI80510.1"
                     /translation="MIKIKRIYDSSGDEDGCRVLVDRLWPRGLSKESARVDVWMKEIA
                     PSAILRKWFCHDTEKWEEFTKLYTRELTENRQMLLEIKRLEKEHKTVTLLYASKDTEH
                     NQAQVLYRELKKIEASE"
     gene            complement(1415..>2332)
                     /locus_tag="PLI30_13005"
     CDS             complement(1415..>2332)
                     /locus_tag="PLI30_13005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019539077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HOI80511.1"
                     /translation="YSYTSLNRPGTLYEYNIATGEAVKLKEQEIPSGFNPDDYVVERL
                     WATAPDGVKVPMAIVYKKGLKKDGTNPALIYSYGSYGSSSDVSFSASLYSLIDRGFVY
                     ALAQIRGGSDLGEQWYEEGKLLHKKNTFTDFIACSELLVNEKYTSTNKLAAMGGSAGG
                     LLMGAVTNMRPDLYQTIVAQVPFIDVINTMLDDTLPLTTGEYEEWGNPNEEEYYNYML
                     SYSPYDNIKAQNYPNMLITGGINDSQVLFHEPTKYVAKLRALKTDDNLVLLHMNMDSG
                     HGGATGRYDGIKDTAFEFAFILNRVGIEK"
BASE COUNT          568 a          490 c          538 g          736 t
ORIGIN      
        1 tggcatccag cacaaaacat gcggtagttt gttgcagcgt cgcgggcctg agttgctcga
       61 ggcatatttt tacaggaatg agctaattga attattatca ggacaagggc cagcagtaag
      121 aataaatttc tatttttcat aggacaattt tttgttaatc aggagagaag gcaaaaataa
      181 cataaaattt ctaattaacg agaattcatc aaaaaattta gcgatgttga tagagactgt
      241 tttgaatttt gatttcttta aaataacccg ctattctgta cgaactgaaa tgtcaaatcc
      301 atcaaaaaca ggcataaaac agggcatgaa aaattcaaaa cagtttcatt ttctccgatt
      361 gtttcccgaa aatcccttac ctttgtgcgt tttttgaaaa atagttgtgg attatatttc
      421 gataatgctt attgccattg gcctggctat ggattcgttt gccgtttgtg tcgggaaagg
      481 aatgtgccgg cagcgttttt acctgtggag atcgttgaag atagctgccg tttttggatt
      541 gtttcaaggg ctgatgccat ttgttgggta tgtgctcgga ataagttttg cttcatggat
      601 gcagcttttt gaccattggt ttgccttctt tattctttcg gttatcggga taaaaatgat
      661 atacgaaagc tttatctcca aacctcatga ggtatgtgta ggttgcgatt gtcaggaaga
      721 tattgttgtt gattggagaa aagtagttgt actggctttt gctaccagta tagacgccat
      781 ggctaccggg ctagttttcg tttcttatcc gggaaccata tcaacggcaa cgataaccat
      841 aggtttggtt accttgttct tcgctttttc gggaatgttt ataggagtac gatttggccg
      901 gaggttacgg ctcaacatcg aaatgctggg cggtatcatc ctgattgcga tagggatgaa
      961 aattcttttc cagcatctcc tgcacggggc gtgataacta cacttgcagg tttctttatt
     1021 cgcttgcctc tattttcttt agctcccggt atagtacttg cgcctgatta tgctctgtgt
     1081 ctttggaagc gtaaagaagc gtcaccgtct tgtgttcttt ctccagtctc tttatttcca
     1141 gaagcatctg tctgttctca gtaagttccc gggtgtagag cttggtgaat tcttcccact
     1201 tttccgtgtc gtgacagaac catttccgta aaatcgcaga tggagcgatt tccttcatcc
     1261 atacatcaac ccgggcgctt tctttcgata ggccgcgtgg ccagagacga tccaccaaca
     1321 cgcggcagcc gtcttcatct cctgaagaat cgtatatgcg ttttattttt atcataagcg
     1381 ataaaccgtt tagcggtaag tttggtttga tacactactt ttcaatcccg actctgttca
     1441 aaataaaagc aaactcaaaa gctgtatctt taatcccgtc gtaacgccct gtagcgccgc
     1501 cgtgtcccga atccatattc atgtgcaata gcacgaggtt gtcatcggtt tttagggcac
     1561 ggagtttggc aacatatttg gtgggttcgt gaaaaagcac ttgcgagtcg ttaatgccac
     1621 cagtaatgag catattggga tagttttgcg ctttaatgtt gtcgtaaggc gagtaggaga
     1681 gcatgtagtt gtaatattct tcttcgttgg gattgcccca ttcctcgtat tcacctgtag
     1741 tgagtggaag cgtgtcatcc agcatggtgt tgataacgtc tataaacggt acttgtgcca
     1801 cgatggtttg atacagatcc ggacgcatgt ttgtgacggc ccccatcagc aggccgcccg
     1861 cgctaccgcc catagctgcg agcttatttg tagatgtgta tttttcgttg accagcagtt
     1921 cactgcacgc gataaaatcg gtaaacgtgt ttttcttgtg gagcagttta ccctcctcgt
     1981 accattgttc tccaaggtca cttcctccac gtatctgagc cagagcatac acgaagccgc
     2041 gatcaatgag gctgtaaagg ctagcgctga aagacacatc ggaactactt ccgtagcttc
     2101 cgtaggaata gatcaacgcg gggttggttc cgtccttttt taatcctttc ttgtacacga
     2161 ttgccatagg cactttcact ccatcggggg cagttgccca aagacgctca accacataat
     2221 catcgggatt gaagcccgaa ggaatttcct gttctttaag ttttaccgcc tcacctgtag
     2281 caatattgta ttcgtacagt gtgccggggc ggttgagtga tgtataggaa ta
//
LOCUS       DAOSDR010000439         2317 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrimonas sp. isolate AMR_MDS_1826
            NODE_16105_length_2317_cov_3.408488, whole genome shotgun sequence.
ACCESSION   DAOSDR010000439 DAOSDR010000000
VERSION     DAOSDR010000439.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863355
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrimonas sp. (wastewater metagenome)
  ORGANISM  Petrimonas sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Dysgonomonadaceae; Petrimonas.
REFERENCE   1  (bases 1 to 2317)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2317)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:10:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,720
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 35
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2317
                     /organism="Petrimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16105_length_2317_cov_3.408488"
                     /isolate="AMR_MDS_1826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2023866"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..445)
                     /locus_tag="PLI30_13010"
     CDS             complement(<1..445)
                     /locus_tag="PLI30_13010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019539138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HisA/HisF-related TIM barrel protein"
                     /protein_id="HOI80512.1"
                     /translation="MRIIPAIDIIDGKCVRLTKGDYSTQKIYNENPVEVAKSFEANGI
                     RYLHLVDLDGAKSKHIVNKDILRKISTKTSLTVDFGGGIKTDEDVRIAFENGAAQVTV
                     GSIAAQEPDLFLKWLSVYGPDKIILGADSHGRKIATQGWQQQSELD"
     gene            complement(463..1044)
                     /gene="hisH"
                     /locus_tag="PLI30_13015"
     CDS             complement(463..1044)
                     /gene="hisH"
                     /locus_tag="PLI30_13015"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019539139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HOI80513.1"
                     /translation="MVAIIKYNAGNIASVKNAVERLGHQCVITDSEVEIRRADKVIFP
                     GVGEASSAMEYLKERKLDAVIKTLGQPVLGICLGLQLLCRYSEEGNTGCLGIFDTAVK
                     KFPLTDIVPHIGWDNLKHTESLLFDGFGKDDTVYFVHSYYAESCAQTIAECDYILPFS
                     AAMRNRNFYATQFHPEKSANVGERILKNFLKIR"
     gene            complement(1050..2141)
                     /gene="hisB"
                     /locus_tag="PLI30_13020"
     CDS             complement(1050..2141)
                     /gene="hisB"
                     /locus_tag="PLI30_13020"
                     /EC_number="3.1.3.15"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008157742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004401 - histidinol-phosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0004424 - imidazoleglycerol-phosphate
                     dehydratase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     histidinol-phosphatase/imidazoleglycerol-phosphate
                     dehydratase HisB"
                     /protein_id="HOI80514.1"
                     /translation="MKKVLFIDRDGTLVIEPPVDFQIDSLEKLEFYPQVFQYLSRIAR
                     ELEYELVMVTNQDGLGTPSFPEDTFWPAHHKMVKAFENEGVVFSEILIDRSFEHENLP
                     TRKPGIALLTHYTKGGYDLENSYVIGDRKTDVRLARNLGCKAIYLNVDKTDDAVLCTT
                     DWAEIYAYLKLEPRKGIVRRKTSETDIVVEINLDGSGKSRIATGLGFFDHMLEQIARH
                     GNVDLSIRVNGDLHIDEHHTVEDTAIALGEAFLQALGKKKGIERYGFLLPMDDCLAQV
                     AIDFGGRPWLVWEVDFKRERIGDVPAEMFMHFFKSFSDNARCNLNIKAEGDNEHHKAE
                     AIFKAFAKSIKMAVKRGENTGIPSTKGIL"
     gene            complement(2138..>2317)
                     /locus_tag="PLI30_13025"
     CDS             complement(2138..>2317)
                     /locus_tag="PLI30_13025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019539141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HOI80515.1"
                     /translation="SDANFLLVEVSDANWIYNELVQKKVIVRNRNRQVTNCVRITIGT
                     PGENEELLNKLRTLV"
BASE COUNT          560 a          608 c          476 g          673 t
ORIGIN      
        1 catccagttc cgattgctgc tgccatccct gggtggcaat ttttctgccg tggctgtctg
       61 cacctaaaat aatcttgtcc ggaccgtata ccgacagcca ttttaaaaaa aggtccggtt
      121 cttgagcagc tatgcttccc accgtaacct gtgcagctcc gttttcgaaa gcgatgcgta
      181 catcttcatc ggttttaata cctccgccaa aatcaaccgt caatgaagtt tttgttgata
      241 tttttctcag aatatcttta ttcacaatat gttttgattt ggctccatcc aaatcaacca
      301 agtgtaaata tcgaattccg ttggcttcaa acgattttgc aacttccacc ggattttcgt
      361 tatatatttt ttgggtgctg taatcgcctt tggtcaggcg gacacacttt ccgtcaataa
      421 tgtctatggc aggaataatt ctcataaact tcaatcgttt gtttacctta ttttcaaaaa
      481 attcttcaat attcgttcac cgacattcgc cgatttctcg ggatggaact gcgtggcata
      541 aaaattcctg ttccgcatgg cggcactgaa cggcaaaata taatcgcact cggcgatagt
      601 ttgcgcacag ctctcggcat aataactgtg tacgaaatag accgtatcat cctttccaaa
      661 accatcaaaa agcaatgact ccgtatgctt caggttgtcc catccgatgt ggggaacaat
      721 gtccgtcaac gggaattttt ttaccgcagt atcaaagata cccaggcagc cggtgtttcc
      781 ctcttccgaa taccggcaca gcagttgcag gcccaggcat attcccagaa cgggctgccc
      841 gagcgttttt attaccgcat ccagcttccg ctcttttaaa tattccatgg ccgacgatgc
      901 ttcgcccaca ccgggaaaaa tcaccttatc cgcacggcgg atttcaacct cgctatcggt
      961 aatgacgcac tgatgcccca accgctccac cgcatttttt accgacgcga tgttgccagc
     1021 attgtatttg atgatagcaa ccattgacat tataatattc cttttgtgct gggaattcct
     1081 gtattttcac cacgtttcac cgccatcttg atcgatttgg cgaatgcttt gaaaatggct
     1141 tccgctttgt gatgttcgtt atcgccttcg gctttgatgt taaggttaca cctggcgtta
     1201 tcggaaaacg acttgaagaa gtgcataaac atttcggcag gcacatctcc aattctctcg
     1261 cgtttaaaat ccacctccca gaccagccaa ggccggccgc cgaagtcgat agcaacctgt
     1321 gccaggcaat cgtccatggg cagcagaaac ccgtaacgtt ctataccctt cttcttcccc
     1381 agtgcttgta aaaaagcctc tcccagggcg atggccgtat cttcgaccgt atggtgttcg
     1441 tcgatatgca aatcaccgtt tacccggatg gagaggtcca catttccgtg acgggcaatt
     1501 tgctccaaca tatggtcgaa aaaccctaat cctgtagcta tccggctttt tcctgatccg
     1561 tcaagattga tctccacaac gatatcggtt tctgaagttt tgcggcggac aattcctttt
     1621 ctgggttcca acttcaagta ggcgtatatc tcggcccagt cggtcgtgca cagcacggca
     1681 tcatcggttt tatccacgtt caggtaaatg gctttgcagc caaggtttct ggccaaccgc
     1741 acatcagttt tccggtctcc gataacgtaa gagttttcca aatcgtaacc cccttttgtg
     1801 taatgtgtca gcaacgctat ccccggtttt cgggtaggaa ggttttcgtg ttcaaaagag
     1861 cggtcgataa ggatttcgct aaaaacaacc ccttcgtttt cgaatgcttt taccatttta
     1921 tggtgtgccg gccagaacgt atcttcggga aaagacggag ttcccaaccc gtcctgattc
     1981 gtcaccataa ccaattcata ttccaattct cgggcaatcc gcgacaggta ctgaaatact
     2041 tgcgggtaaa actccagctt ctccaggctg tcaatctgga agtcaacggg cggctcgata
     2101 accagtgttc cgtcccggtc gataaataaa acttttttca tacaagggtg cgtaatttat
     2161 tcaacaactc ctcattctca ccgggagtgc cgatggtaat tctcacacag tttgtcacct
     2221 gcctgttccg gttacggaca atcacctttt tttgtaccag ttcgttgtaa atccagttgg
     2281 catctgatac ttcaaccaac aggaagttgg catccga
//
LOCUS       DAOSDR010000440         2313 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrimonas sp. isolate AMR_MDS_1826
            NODE_16132_length_2313_cov_5.586360, whole genome shotgun sequence.
ACCESSION   DAOSDR010000440 DAOSDR010000000
VERSION     DAOSDR010000440.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863355
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrimonas sp. (wastewater metagenome)
  ORGANISM  Petrimonas sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Dysgonomonadaceae; Petrimonas.
REFERENCE   1  (bases 1 to 2313)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2313)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:10:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,720
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 35
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2313
                     /organism="Petrimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16132_length_2313_cov_5.586360"
                     /isolate="AMR_MDS_1826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2023866"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1935)
                     /gene="metG"
                     /locus_tag="PLI30_13030"
     CDS             complement(<1..1935)
                     /gene="metG"
                     /locus_tag="PLI30_13030"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008646565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HOI80516.1"
                     /translation="MSKQFKRTLITSALPYANGPVHIGHLAGVYVPADIYARYLRLKG
                     EETLFVGGSDEHGIPITIRARKEGVTPQDIVDRYHELIKKSFEEFGISFDIYSRTTSD
                     IHKKTASDMFLKIYENDGFQEIESEQYYDEEAGQFLADRYITGTCPHCSNQRAYGDQC
                     EQCGTSLSPTDLINPKSALSGSIPVMRTTKHWYLPLNEHEAWLREWILTEHKEWKSNV
                     YGQCKSWLDLGLQPRAVSRDLDWGIPVPLKDAEGKVLYVWFDAPIGYISNTKELLPND
                     WEKWWKDESTKLVHFIGKDNIVFHCIVFPAMLKAEGSYILPENVPANEFLNLENDKIS
                     TSRNWAVWLHEYLEEFPGKQDVLRYALTANAPETKDNDFTWKDFQARNNNELVAVLGN
                     FINRALVLTQKYFNNLVPAPGELSGYDRETIAECQQVKQAVEYNLESYHFREAQKEAM
                     NLARIGNKYLADTEPWKTAKTDSARTATILNLSLQIVANLAIVCEPFLPFSTKKIYAF
                     IHAKKFDWEKLGSFDLLPEGHELGKAELLFEKIEDETIEKQVEKLHATKAANEQEAYR
                     AKPVKDNIIYDDFDKLDIRIGTVLECEKVPKTDKLLRFLLDDGLSKRTILSGIAAYYP
                     HPDQLVGKQVCFIANLEPRKL"
     gene            2108..>2313
                     /locus_tag="PLI30_13035"
     CDS             2108..>2313
                     /locus_tag="PLI30_13035"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HOI80517.1"
                     /translation="MDLNITGKVAIIGGSSKGLGKACAVALAKEGVNIVLCARRKETL
                     QRTKSEIELLGVEVLALPVDMASA"
BASE COUNT          555 a          495 c          564 g          699 t
ORIGIN      
        1 caatttcctc ggttccaggt tggcaataaa gcaaacctgt ttcccgacca gttggtcggg
       61 atgggggtag tatgccgcaa ttcccgataa aatggtgcgc ttgctgagac catcgtcaag
      121 caggaatctc agcaatttat cggtcttagg cactttttca cattccagaa cggttcctat
      181 gcggatatcc agcttgtcga aatcatcgta aatgatgttg tctttgacgg gtttagcccg
      241 gtacgcttcc tgttcgtttg cagccttggt tgcgtgaagt ttttccactt gcttttcaat
      301 ggtttcatct tcaattttct caaaaaggag ttctgctttt cccaattcgt gcccttcagg
      361 aagtaggtca aaactcccta acttttccca atcgaatttc ttggcatgta taaacgcgta
      421 gatcttcttt gtcgaaaaag gcaggaaggg ttcgcatacg atggccagat tggcaacgat
      481 ctgtagcgat aggttcagga tagtggccgt gcgtgcgctg tcagtttttg ccgttttcca
      541 cggttcggta tctgccaggt atttgtttcc gatgcgtgcc aggttcatcg cttctttttg
      601 tgcttcgcgg aagtggtagc tctcgagatt atattctaca gcttgtttca cttgctggca
      661 ttcggcaatg gtttcgcggt cgtaaccgga gagttctcct ggagcaggaa caaggttgtt
      721 gaaatacttc tgcgtaagta ccaatgcgcg attaatgaaa ttgcccagaa cagcaaccag
      781 ttcgttgttg ttgcgtgcct gaaaatcctt ccacgtgaaa tcgttgtctt tggtttcggg
      841 tgcgtttgca gtaagcgcat agcgcaacac gtcctgttta cccggaaatt cttcaaggta
      901 ttcgtgcagc cataccgccc agttgcgtga tgtggatatt ttatcgtttt cgaggtttaa
      961 aaattcgttt gcgggaacat tttcgggcaa gatgtaagag ccttctgctt taagcattgc
     1021 cgggaaaaca atacagtgga acacaatgtt gtctttcccg ataaaatgaa ccagtttggt
     1081 gctttcgtct ttccaccatt tttcccagtc gttgggtagc agttccttcg tattggaaat
     1141 ataaccgatg ggcgcatcaa accagacgta aagcactttc ccttcggcat ctttcaacgg
     1201 aacaggtatt ccccaatcca ggtcgcggct tacggcccgc ggctgcaagc ccaggtcgag
     1261 ccacgatttg cattgtccgt atacgttcga tttccactct ttatgctccg tcaaaatcca
     1321 ttcacgcagc catgcttcgt gttcattgag cggtaaatac cagtgtttgg ttgttcgcat
     1381 aacgggtatg cttccgctta aagccgattt agggttaatt aaatcggttg ggctaagcga
     1441 agtgccgcat tgttcacact ggtcgccgta agcgcgttgg ttgctgcagt gtgggcaggt
     1501 tccggtgatg tacctatcgg caaggaattg tccggcctct tcatcgtaat actgttcgct
     1561 ttcaatttct tgaaatccat cattttcata gattttcagg aacatatccg atgccgtttt
     1621 tttgtgaata tcggatgtgg tgcgtgaata aatgtcgaat gaaattccaa actcttcaaa
     1681 cgattttttg ataagctcgt gataccggtc cacaatgtcc tgaggtgtaa caccttcctt
     1741 ccgggcacga atggtaatag ggattccgtg ttcatcagaa ccacccacaa aaagggtctc
     1801 ttctcctttc aggcgtaaat acctggcgta gatatctgcc gggacataaa ctcccgctaa
     1861 gtgtccgatg tgaaccgggc cgtttgcata tggcaatgcc gatgtgataa gtgtgcgttt
     1921 aaattgtttt gacataaaat agagttgtgt gttccctgtt ccggattcca tgtttacaga
     1981 ccgataatac cgaaaaggga atcgtttttt tgaagtgcaa agatacgaaa aatagctgct
     2041 ttttgattat cgtctttgaa atataaaaca agaacgttgg tttagcgtta ttaaagaaaa
     2101 aaagtatatg gatctgaata taacaggtaa agtggccatt atcggtggaa gcagcaaagg
     2161 gctgggaaag gcgtgtgcgg tagcattagc aaaagaaggt gtgaacattg tattgtgtgc
     2221 ccgccgtaaa gaaaccttgc agagaaccaa aagcgaaata gagttgctgg gagttgaggt
     2281 gttggctttg ccggttgata tggcttctgc tga
//
LOCUS       DAOSDR010000441         2312 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrimonas sp. isolate AMR_MDS_1826
            NODE_16144_length_2312_cov_3.167922, whole genome shotgun sequence.
ACCESSION   DAOSDR010000441 DAOSDR010000000
VERSION     DAOSDR010000441.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863355
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrimonas sp. (wastewater metagenome)
  ORGANISM  Petrimonas sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Dysgonomonadaceae; Petrimonas.
REFERENCE   1  (bases 1 to 2312)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2312)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:10:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,720
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 35
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2312
                     /organism="Petrimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16144_length_2312_cov_3.167922"
                     /isolate="AMR_MDS_1826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2023866"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2263
                     /locus_tag="PLI30_13040"
     CDS             <1..2263
                     /locus_tag="PLI30_13040"
                     /inference="COORDINATES: protein motif:HMM:NF024754.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HOI80518.1"
                     /translation="NLSYNTAVSILQNYHTLGILNDIRNRLQEYQQENNTLFLSDTTE
                     LLNRIVSDSESPFIYEKTGTRINHYMIDEFQDTSSMQWQNFKPLIKESLDGGHFNLIV
                     GDVKQSIYRWRNSDWELLESRILQDFSDNIIRNNVLEMNWRSDFNIVNFNNSVFSAAA
                     AILQKKFNTSAELQENPNGKITEAYQHVRQQVPSGKSPGEGYVKISFLDTSDKENDWK
                     SKALEQLPLEIERLQDQGFALKDIAILVRKNDEAIEVAETLLNYKEKNLQSGYRYDII
                     SNEALVIGNAQSVKAVIALLRHFQNRNDETRRMLAVYEYYRFHRGLLPDKAISNYFDE
                     GAKDFPVEVKPELHRISTLPFYEMIEAFFAMSVDALNEKENAYVQAFMDIVLKFSTDA
                     SSNINDFLEWWDEKGSGKTLFSPDEQDAIRLVTIHKSKGLGFGAVIMPFLKWDIDHTG
                     NMGPVLWCKPTVEPFNRLGTIPLQYKSSLENTLFKEDYLKEKLYTYIDNLNLLYVAFT
                     RAKNRIIAVAPKPVGDAVNDINSLLWQTFKSDGKNEGELLLSDYLTGQENNAVFELGT
                     PTLLQRKTEKGSASELDSGKWQSIPFNNRLKLRLNSAGFFSEDGSRAYGTLMHEIISS
                     IQDLGDLAGAVEKKYISGEIDAGEKNEITGLLTRILSDPGIAEWYSGKYRVINETEVL
                     HPAFGFSRPDRIMIGDNEAIVVDYKFGEREEEQYKRQVRHYVRLIKEMGYNNVKGYVF
                     YVKIGKVIEVGTI"
BASE COUNT          693 a          541 c          558 g          520 t
ORIGIN      
        1 caacctctcc tacaacaccg ccgtaagtat cctgcaaaac taccacacgc tggggatcct
       61 caacgacata cgaaaccgcc tgcaggaata ccagcaggaa aacaacaccc ttttcctttc
      121 cgacacaacc gaactgttga acaggattgt ttccgacagc gaatcgccct ttatctacga
      181 aaaaacaggc acgcgcatca accactatat gattgatgag ttccaggata cttcatccat
      241 gcaatggcag aattttaaac cgttaatcaa ggagagcctg gacggcggac atttcaacct
      301 gattgtcggg gacgtgaaac aaagcatata ccgttggcgg aattccgact gggagttgct
      361 agaaagccgg atcctacagg atttcagcga caacattatc cgcaacaacg tgcttgaaat
      421 gaattggcgt agtgatttca acatcgtgaa tttcaacaat tccgtctttt ccgccgcagc
      481 ggcaatcttg caaaaaaaat tcaatacctc tgcagaatta caggagaatc ccaacgggaa
      541 aattacagaa gcctaccaac acgtccggca acaggttccg tcgggtaaaa gtccgggcga
      601 ggggtatgtc aaaatttcgt tcctcgacac ctctgacaag gaaaacgact ggaaatcaaa
      661 ggcattagaa cagcttcctc tcgagatcga gcgcttgcag gaccagggat tcgccttaaa
      721 agacattgcc atcctggtgc ggaagaacga cgaagcaata gaggttgccg agaccctgct
      781 gaactacaag gaaaagaacc tccaatccgg ttaccggtac gacatcatat caaacgaggc
      841 actcgtcatc ggtaacgcgc aaagtgtgaa ggccgtcatt gccttgctcc gccattttca
      901 gaaccgcaac gatgaaaccc gccgcatgct tgccgtatat gaatattacc gatttcaccg
      961 gggcctgttg cccgataagg ctatcagcaa ctattttgat gaaggagcga aggatttccc
     1021 cgttgaagtc aaaccggaac ttcaccgcat ctccaccctg cctttttacg agatgatcga
     1081 ggcttttttc gcgatgagtg tggatgcgct caatgaaaag gaaaatgctt acgtacaggc
     1141 ttttatggat attgtgctga aattcagcac cgatgcttca tcgaacatca atgattttct
     1201 ggaatggtgg gacgaaaaag gatcggggaa aacacttttt tcgcccgacg aacaagatgc
     1261 catccggttg gtcaccatac acaaatccaa gggattgggg tttggtgccg ttattatgcc
     1321 gttcttaaaa tgggacatcg accataccgg caatatgggg cctgttttgt ggtgtaagcc
     1381 cacggtggaa ccttttaaca ggcttggaac cattccactg cagtataaga gctctctcga
     1441 aaacaccctc ttcaaagagg actacctgaa ggagaagctg tacacataca tagacaacct
     1501 gaatctgctt tatgtggctt tcacacgcgc caagaaccgc atcatcgccg ttgcgcctaa
     1561 acctgtaggg gacgccgtta acgatatcaa ttcgctgttg tggcagacat ttaaatcgga
     1621 cgggaaaaat gaaggagagc ttttgctaag tgattatctc accggacagg aaaacaacgc
     1681 ggttttcgag ttgggaaccc ctactctgct ccagcgtaag acagaaaaag gatcggcttc
     1741 cgagctggat tcaggaaaat ggcaatccat cccgttcaac aaccggctta agctgcggtt
     1801 aaactcagca ggcttttttt ccgaagatgg aagccgtgcc tacggaacac ttatgcacga
     1861 gatcatcagt agcatacaag accttggcga cctggccggt gctgttgaaa aaaaatatat
     1921 ttcgggggaa atcgatgccg gtgaaaaaaa cgagataacc ggactgctca cccgcatttt
     1981 gtcagatccg gggattgctg aatggtattc aggaaaatac cgtgtcatca acgaaacaga
     2041 agtacttcat ccggctttcg gctttagccg gcccgacagg ataatgattg gagataacga
     2101 ggcaatcgtg gtggactaca aatttggcga aagggaagaa gaacaataca aacgccaggt
     2161 gagacattac gtcaggttaa taaaagagat gggatacaac aatgtgaaag gatacgtgtt
     2221 ttacgtgaaa atagggaaag taatcgaagt cgggacaatc taaagattat gctaaaagaa
     2281 atgctatatg tgggattggg gggtggagcc gg
//
LOCUS       DAOSDR010000442         2284 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrimonas sp. isolate AMR_MDS_1826
            NODE_16377_length_2284_cov_2.502019, whole genome shotgun sequence.
ACCESSION   DAOSDR010000442 DAOSDR010000000
VERSION     DAOSDR010000442.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863355
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrimonas sp. (wastewater metagenome)
  ORGANISM  Petrimonas sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Dysgonomonadaceae; Petrimonas.
REFERENCE   1  (bases 1 to 2284)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2284)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:10:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,720
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 35
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2284
                     /organism="Petrimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16377_length_2284_cov_2.502019"
                     /isolate="AMR_MDS_1826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2023866"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2284)
                     /locus_tag="PLI30_13045"
     CDS             complement(<1..>2284)
                     /locus_tag="PLI30_13045"
                     /inference="COORDINATES: protein motif:HMM:TIGR04056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0071702 - organic substance transport
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SusC/RagA family TonB-linked outer membrane
                     protein"
                     /protein_id="HOI80519.1"
                     /translation="LRSNSVTVSQLISEIEKQTDYLVVYSNREVNTSRTISLKNKSDK
                     VSEYLNQTFSGTDIGYDFEKNYIVLSKKAQQTSTTITNLVQTLQQQGKTVRGTVTDSN
                     GEPVIGATIVVKGNPSQGTVTDIDGKFILSNLSENTVLQITYVGMKPQDISTVGRNTI
                     DAVMEADVESLDELVVVGYGTQKKTSLTSSVAAIKGKELTDIPLTDLSAGIGGRLSGV
                     IVKQNTGEPGRDGARINIRGISTTGSSTPLLIVDGIPRGFQQLDPNNIESFTVLKDAA
                     AVAPYGVAGANGVILVTTKSGTENKPTLTYHGYIGFQSPTILPDQVNSYEYAILKNVG
                     AVNIGQPKPYSDEVLQKLKDGSDPDAYPPYNDLYGELIKYNSPLTKHNIEISGGTEKF
                     KYYGSVGYMYQDGMWQAKYTPAKSNQYNVTMNVDAKVTDITNVSLRLNGSVKDASRPP
                     SDLWDWGTDRIFELIKYANLQSGPLWYSNGMAGTYVSAALTTSGTFKRLNTNIFTQLE
                     ITQEIPFISGLKLSGLIAYDPSWDNNKTWRTPVHLASINKSKLPYVITDGIFGDTKPS
                     LRQMWDQYQQLTFQVKANYTKQLGKHNFTFMSVFEVKNNHMESLNAYRRNYDVFIDEI
                     DMGSSSNKDISNGGTSSDAKQIGFVYRLNYDYLSKYLLEVTGRYDGHYYFAPENRFGF
                     FPAFSVGWRVSEEEFLKNTAWLSNLKLKASYGEVGALAGGPYQYMSLYKVSGPGYVFG
                     SSGVSVAVENVEPNKKITWER"
BASE COUNT          631 a          472 c          405 g          776 t
ORIGIN      
        1 cgttcccatg taattttttt gttgggttct acattttcta cagcaaccga aacgccgctg
       61 cttccaaata catatcccgg gcccgaaact ttataaagcg acatatactg ataaggtcct
      121 cctgccagtg cacctacctc tccataagaa gctttaagct ttaaattgga caaccatgct
      181 gtgtttttca aaaattcctc ttccgatact cgccaaccaa cggaaaaagc aggaaaaaag
      241 ccaaaacgat tttcgggagc aaaataataa tgcccatcgt aacgtcctgt aacttcaaga
      301 agatatttac tgagataatc ataatttaaa cgatatacaa aacctatctg ttttgcatca
      361 cttgaagttc ccccattact tatgtcttta tttgaagagc tacccatatc aatttcatcg
      421 ataaaaacat cataatttct tctataagca tttaaactct ccatatggtt attttttacc
      481 tcaaaaacgc tcatgaatgt aaaattatgc tttcctaatt gttttgtata attagctttt
      541 acctgaaaag tcaattgttg atattgatcc cacatttgac gtaaagatgg tttcgtatca
      601 ccaaaaattc catcggtgat aacgtaaggt aattttgact tattaattga agctagatgg
      661 acaggggttc tccatgtttt attgttatcc caactcgggt cgtatgctat taatccactt
      721 agttttaaac cggaaataaa gggtatttct tgtgtaattt ctaattgagt aaaaatattc
      781 gtgtttaggc gcttgaaagt tccactcgta gttaatgcag cggaaacata agtgccggcc
      841 attccattac tataccaaag tggcccactt tgtaaatttg catattttat aagttcaaag
      901 attctgtccg ttccccaatc ccatagatct gaaggaggac gagacgcgtc ttttacagat
      961 ccgttgagtc tcaatgaaac atttgtaata tcggtaactt ttgcatccac attcatggtt
     1021 acattatatt gattcgattt tgctggtgtg tattttgcct gccacattcc gtcctgatac
     1081 atataaccta cacttccgta atatttgaat ttttcagttc ctcctgatat ttctatatta
     1141 tgctttgtta aaggagaatt gtatttgatt aattcgccgt ataaatcatt ataaggagga
     1201 taggcatcgg ggtcggagcc atctttaagt ttctgtagta cttcgtcact atacggtttg
     1261 ggttgtccta tatttacagc tccaacattc tttaaaatag cgtattcgta actgttaacc
     1321 tgatcaggca gtatggtagg gctttgaaat cctatatagc catgataagt taatgttggt
     1381 ttgttctccg taccactttt cgtagttacc agaattacac cgttggctcc ggcaactccg
     1441 taaggtgcaa cggccgcagc atcttttaac accgtaaatg attcaatatt gttcggatcc
     1501 aattgctgaa acccacgagg aatgccatcc actatcagca aaggagtaga ggagcctgta
     1561 gtggaaattc ctcggatatt aatacgtgcg ccatctcttc ccggttctcc agtattttgt
     1621 tttacaatta cacccgacaa ccgacctcct attccagcac ttaaatcggt taaagggatg
     1681 tctgtaagtt cttttccctt tatagccgct acggaagacg ttaaagatgt ttttttctgc
     1741 gtcccatacc caacaaccac aagttcatct aacgactcca catctgcttc cattacagca
     1801 tctattgtgt ttcttcctac agtgctaata tcctgaggtt tcatgcctac ataagtgatt
     1861 tgtaatacag tattttccga taaattagat aaaatgaatt taccatcaat atctgtaact
     1921 gtaccttgcg aagggttacc ttttacaaca attgttgccc caataacggg ttctccgttt
     1981 gaatcggtaa cggtacccct cacggttttt ccttgctgtt gaagagtttg caccaaattt
     2041 gtaattgtgg ttgaggtttg ttgggctttt ttcgaaagga caatgtagtt tttctcaaaa
     2101 tcgtaaccga tatctgtccc gctgaatgtt tggttcaggt attcggatac tttgtccgat
     2161 ttatttttaa gacttattgt gcgcgaggtg tttacttcgc ggttgctgta aacgaccaga
     2221 tagtcagttt gtttttcaat ttcactgatt agctgactta cggttacaga gttggatctc
     2281 agtt
//
LOCUS       DAOSDR010000443         2266 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrimonas sp. isolate AMR_MDS_1826
            NODE_16542_length_2266_cov_3.691995, whole genome shotgun sequence.
ACCESSION   DAOSDR010000443 DAOSDR010000000
VERSION     DAOSDR010000443.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863355
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrimonas sp. (wastewater metagenome)
  ORGANISM  Petrimonas sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Dysgonomonadaceae; Petrimonas.
REFERENCE   1  (bases 1 to 2266)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2266)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:10:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,720
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 35
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2266
                     /organism="Petrimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16542_length_2266_cov_3.691995"
                     /isolate="AMR_MDS_1826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2023866"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2022)
                     /locus_tag="PLI30_13050"
     CDS             complement(<1..2022)
                     /locus_tag="PLI30_13050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019540240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tex family protein"
                     /protein_id="HOI80520.1"
                     /translation="MNSPVIPSLISNALNIRLNNVENTIKLLEGGATIPFISRYRKEA
                     TGSLDEVQVSEIKSLNDKFIELEKRKKTVLSSIEEQERLTPELQERIENCWDAVALED
                     IYLPYKPKRQTRAEMARKRGLEPLAKWLMLQQYGPVEAKAGQFAGEELDDAVSALKGA
                     CDIIAEWVNEDEKARQLIRNIFAREAVITAKVVKGKEQEGEKYRDYFDFSTLLSRCPS
                     HRLLAIRRGESEGFLRVSISPDDEKCLDKLVPRYAKNDSEAADYVEKAVEDAYKRLLK
                     PSIETEFANLSKEKADEAAIAVFAENLRQLLLSPPLGQKRILAVDPGYRTGCKLVCLD
                     EQGNLLHNETIYPHPPQNEFSKSAGKVSKLVSTYRIDAIAIGNGTASRETERFIANLR
                     YDREVKVFVVSENGASVYSASKTAREEFPEYDVTVRGAISIGRRLSDPLAELVKIDPK
                     SIGVGQYQHDVDQAKLKRSLDQTVESCVNLVGVNLNTASKHLLMYVSGLGESLAQNIV
                     NYRAENGPFRARAGLRKVPRLGEKAFEQCAGFLRIPDAENPLDNSAVHPESYPVVERM
                     AKDLKCSVKEVISNKALVGTIDINRYKTDTIGTETLTDILQELEKPGRDPRTKVQVLE
                     FDPSIRTIADVKEGMVVNGIITNITNFGCFVDFGIKENGLVHISELAGRF"
     gene            2107..>2266
                     /locus_tag="PLI30_13055"
     CDS             2107..>2266
                     /locus_tag="PLI30_13055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019540241.1"
                     /note="Sms; stabilizes the strand-invasion intermediate
                     during the DNA repair; involved in recombination of donor
                     DNA and plays an important role in DNA damage repair after
                     exposure to mutagenic agents; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HOI80521.1"
                     /translation="MAKLKSTYFCTNCGNESPKWMGKCPACGEWGSLVEELVRKDAPS
                     RVTDTRSFE"
BASE COUNT          529 a          602 c          531 g          604 t
ORIGIN      
        1 gaagcggccg gccagttccg agatatggac caagccgttc tccttgatgc cgaaatccac
       61 gaaacaaccg aagttagtga tattggtaat gattccattt accaccattc cttctttcac
      121 gtctgcgatg gtacggatag aggggtcaaa ctccaacacc tgcactttcg tgcgtggatc
      181 gcggccgggc ttttccagct cctggaggat atcggtcagc gtttcggtac cgatggtatc
      241 tgttttgtag cggttgatat cgatagtacc gacaagcgct ttattactga tcacctcctt
      301 gacggaacat ttaaggtcct tggccatcct ttcaaccacc ggataacttt ccggatggac
      361 ggcagagtta tcgagcgggt tttcggcgtc gggaatccga aggaaaccgg cacattgctc
      421 gaaagctttt tcacccagac ggggtacttt ccttaatcct gcgcgtgcgc ggaaaggacc
      481 gttctcggcc cggtaattca cgatattctg tgccagggac tcacctagac ccgaaacata
      541 catcagcaaa tgtttgctcg ccgtgttaag gtttaccccc acaaggttca cgcaactttc
      601 cacggtttga tccagggaac gtttcagttt tgcctggtcc acatcgtgct gatactgccc
      661 cacccctatg gatttcggat caattttcac cagttccgcg agcggatcac ttaacctcct
      721 gccgatagag attgccccgc gcactgtaac atcatactcc gggaactctt cacgtgccgt
      781 ctttgaggcc gaatatacgg aggcaccgtt ctcactcact acaaaaacct tcacctcccg
      841 gtcgtacctc aggttcgcta tgaagcgctc ggtctcccgg cttgccgtac cgttgccgat
      901 ggcaatcgca tcgatacggt aagtggaaac cagcttggaa accttacccg ccgacttcga
      961 aaattcgttt tgcggcggat gcggataaat ggtttcgttg tgcagtaaat tgccttgctc
     1021 gtccagacaa acaagcttgc atcccgtccg gtatcccggg tcgactgcca gtatccgctt
     1081 ctgccccagt ggaggtgaaa gcagaagctg acgcagattc tcggcaaaaa cagctatagc
     1141 ggcctcgtcg gctttttcct tggagaggtt ggcaaactca gtctcaatgg aaggtttgag
     1201 caagcgttta taggcatcct ccaccgcttt ttcaacgtaa tccgcagcct cactatcgtt
     1261 cttggcgtat cggggcacca gcttatccaa gcatttttca tcgtcgggag agatggatac
     1321 ccgaagaaac ccttccgatt ctccccggcg gatggcaaga agacgatgcg agggacaccg
     1381 ggaaagaaga gtagaaaagt caaaataatc cctgtacttt tcgccttcct gttctttgcc
     1441 tttcaccact ttggcggtta tgaccgcttc acgggcaaaa atgtttcgta tgagctgacg
     1501 ggctttttca tcttcgttta cccattcggc aataatgtca cacgcccctt ttaacgccga
     1561 aacggcatca tccagttctt ctccggcaaa ctgtccggct ttcgcttcaa ccggcccata
     1621 ctgttgcagc atcagccatt tcgccaaagg ctccaatccc cttttcctgg ccatctccgc
     1681 gcgcgtctgc cgtttgggtt tgtaaggcag gtaaatatcc tccaacgcaa cggcatccca
     1741 gcaattttcg atcctctctt gcagttccgg tgtcagtctt tcctgttctt cgatggacga
     1801 cagaacggtt ttcttccgtt tttcgagctc aatgaattta tcattcagtg atttaatttc
     1861 tgaaacctgt acttcatcca acgatccggt tgcttctttc cggtatcggc tgataaaagg
     1921 gatggtagca ccgccttcca acagtttaat ggtattttcc acattattga ggcggatgtt
     1981 taaagcgttg gaaataagtg aagggatgac gggactgttc atttcgaatt tgttttttag
     2041 gatacaaaaa tacaaaaatg ttcagaaggg taaaaatatc gctatctttg taaacaaact
     2101 caacgcatgg ccaaactaaa atcaacctac ttttgtacca actgcggcaa cgaatcgccc
     2161 aaatggatgg ggaaatgccc tgcctgtggc gaatggggat cgttggtgga ggagctggtg
     2221 cgaaaagatg ctccttcaag agttaccgat acacgttcgt tcgaaa
//
LOCUS       DAOSDR010000444         2258 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrimonas sp. isolate AMR_MDS_1826
            NODE_16637_length_2258_cov_3.944167, whole genome shotgun sequence.
ACCESSION   DAOSDR010000444 DAOSDR010000000
VERSION     DAOSDR010000444.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863355
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrimonas sp. (wastewater metagenome)
  ORGANISM  Petrimonas sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Dysgonomonadaceae; Petrimonas.
REFERENCE   1  (bases 1 to 2258)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2258)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:10:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,720
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 35
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2258
                     /organism="Petrimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16637_length_2258_cov_3.944167"
                     /isolate="AMR_MDS_1826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2023866"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..771)
                     /locus_tag="PLI30_13060"
     CDS             complement(<1..771)
                     /locus_tag="PLI30_13060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019539386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOI80522.1"
                     /translation="MQHNLFSSDKFSFFSLGSGSCGNCYYLGNSHYGMLIDAGLGPRI
                     IKKRLGDHGIDFSSIYALLVTHDHYDHIKSAGYLSEKLNIPVYATEKVHRGIRNCPLV
                     KNTINGATKRIIEKGESFQIEDFRITAFDVPHDSSDNAGYFFEFGNHKLTLATDIGAI
                     TDDVAYYAGKANHLVIESNYDEEMLLNGRYPYFLKQRISNGTGHLSNRQTAEFLANNC
                     SPHLRNIWLCHLSGDNNSPDLAYETVKNELVGKGIEVGV"
     gene            complement(833..>2258)
                     /locus_tag="PLI30_13065"
     CDS             complement(833..>2258)
                     /locus_tag="PLI30_13065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006841747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DPP IV N-terminal domain-containing protein"
                     /protein_id="HOI80523.1"
                     /translation="VAIGVYNVKTGQTVYLKTGEPRDKYLTNVVWSPDEKFIYVAELN
                     REQNHLKMNRYDAGSGDFDKTLFEETHPKYVEPENPVLFVRNNPAQFVWQSERDGFNH
                     LYLYDTSGRLLKQLTRGDWEVTDVIGFDGKGQHLFFVSTQPSPLERHAYSVNLKSGSI
                     HRLTNVPGMHTIAVSNSGRYLVSRYSASDNPGKVDIIDLKNAKTVTLTSALNPFAGIS
                     MPRVELGSLKAADGKTDLFYRMVKPANFDAAKKYPVAIYVYGGPHSQLVQNRWRYGSG
                     GWETYMAQKGYLVFVLDNRGTSFRGRAFEDVTHRRLGVEEAKDQMCGVEFLKSLPYVD
                     AGRLGVHGWSYGGFMTINMLLRYPEVFKVGVAGGPVIDWKYYEVMYGERYMDTPQENP
                     DGYAKTSLLNKAGRLKSRLLIIHGDEDPVVVMQHSLQFLKSSIGAGTHPDFFVYPGHE
                     HNMIGRDRVHLHEHITRYFEDFCR"
BASE COUNT          562 a          575 c          495 g          626 t
ORIGIN      
        1 cactcccacc tctatacctt tgccgacaag ttcattcttc accgtctcat atgccagatc
       61 gggactgttg ttatcaccgc tcaggtggca aagccagata ttccggagat gaggtgaaca
      121 gttgtttgcc aaaaactcag cagtctgccg gttgctcaag tgtcccgttc cgttactgat
      181 tcgctgttta aggaaatagg gataccttcc gttaagaagc atttcttcgt cgtaattgct
      241 ttcaataacc aggtggttag cttttccggc gtaataggct acatcatcgg ttatagcccc
      301 gatgtcggtt gccagtgtaa gcttgtgatt accgaactca aaaaaataac cggcattgtc
      361 gctgctgtcg tgcgggacat cgaaagcggt aatcctaaaa tcttctatct gaaacgactc
      421 tcccttctct ataattcgtt tagttgcacc gttgatggtg tttttaacaa ggggacagtt
      481 acgaatgccc cggtgtacct tctctgtagc atagaccggt atattaagtt tttcgctcag
      541 gtaccctgct gattttatgt ggtcgtaatg gtcgtgtgtc accagaaggg cgtaaattga
      601 cgaaaaatcg ataccgtgat cacccaatct ttttttgata atacgtggcc ccaatccagc
      661 atcgataagc atcccgtagt ggctgtttcc caggtaataa caattaccgc aactaccgct
      721 gcccaaactg aagaacgaga atttatcgga agaaaataag ttatgttgca tattctaacg
      781 gacaaaggta cgaacaattt atgatttttg tgcgtaatcc gacctacctt cgctaccggc
      841 aaaaatcctc aaaatagcgt gtgatgtgtt cgtgcagatg cacacggtcc cggcctatca
      901 tgttatgctc gtgtccgggg tagacgaaaa agtcgggatg cgtgcctgca ccaatcgacg
      961 atttcaagaa ctgcaagctg tgctgcatca ccactaccgg atcttcgtca ccgtgaatga
     1021 taagcagcct cgatttcagc ctaccagctt tgttcagcaa acttgtcttc gcgtaaccgt
     1081 caggattctc ctgaggcgta tccatgtaac gttcaccgta catcacttcg taatatttcc
     1141 aatcgatcac aggaccaccg gcaacgccca ccttaaatac ctccgggtag cgcagtaaca
     1201 tgttaattgt cataaatccg ccatagctcc agccgtgaac acccagccgg ccggcatcca
     1261 cgtaaggcag tgatttcaag aattccacac cgcacatctg gtctttggcc tcctccacac
     1321 caagcctacg gtgagtaaca tcctcgaagg ctcgtcctcg aaacgatgta ccccggttat
     1381 ccaacacaaa aacaaggtat cctttctgtg ccatatacgt ttcccaaccg cccgaaccgt
     1441 atctccagcg attctgcacc aattgtgaat gcggaccacc gtaaacatat atggccacag
     1501 ggtatttctt tgccgcatcg aagttagcgg gctttaccat ccggtaaaat aaatcggttt
     1561 taccgtcggc agctttcagg cttcctagtt caacacgagg catactgatt ccggcaaaag
     1621 gatttagagc ggaggttagt gtaaccgttt tggcgttttt caagtcgata atatcaactt
     1681 taccgggatt atcgctggct gaataacggc tgaccaggta tcttccagaa ttgctgacgg
     1741 caatggtgtg catgcccgga acattggtga gtcggtggat gcttccgctc tttaaattta
     1801 cgctgtaagc atgcctttct aaagggctgg gctgggttga gacaaaaaac aggtgttgtc
     1861 cttttccgtc aaaaccgata acatccgtta cttcccaatc tccccgggtg agttgtttca
     1921 gtaatcggcc cgacgtatcg tacaggtaca ggtggttgaa tccgtcccgt tcgctttgcc
     1981 agacaaattg agcggggttg ttcctcacaa acaaaaccgg attttcaggc tccacgtact
     2041 tcggatgtgt ttcttcaaaa agtgttttat caaaatcacc cgaccctgcg tcgtaccggt
     2101 tcatcttcaa gtgattttgt tcacggttaa gttcggctac atatataaat ttttcgtcgg
     2161 gactccaaac cacgtttgtc aggtacttgt ccctgggttc acctgttttc aggtaaacgg
     2221 tttgcccggt ctttacgtta taaacaccga tcgccact
//
LOCUS       DAOSDR010000445         2254 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrimonas sp. isolate AMR_MDS_1826
            NODE_16672_length_2254_cov_3.200091, whole genome shotgun sequence.
ACCESSION   DAOSDR010000445 DAOSDR010000000
VERSION     DAOSDR010000445.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863355
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrimonas sp. (wastewater metagenome)
  ORGANISM  Petrimonas sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Dysgonomonadaceae; Petrimonas.
REFERENCE   1  (bases 1 to 2254)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2254)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:10:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,720
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 35
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2254
                     /organism="Petrimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16672_length_2254_cov_3.200091"
                     /isolate="AMR_MDS_1826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2023866"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            1..690
                     /locus_tag="PLI30_13070"
                     /pseudo
     CDS             1..690
                     /locus_tag="PLI30_13070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004338465.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
     gene            complement(718..1710)
                     /locus_tag="PLI30_13075"
     CDS             complement(718..1710)
                     /locus_tag="PLI30_13075"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019537891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017118 - lipoyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HOI80524.1"
                     /translation="MIYIVNKSNKPDYNIALEEYCFKKLLHHDKVFILWINEPAIIVG
                     KHQNTIEEINSEYVKQNGIHVIRRISGGGAVYHDLNNLNYTIISNEDKGADFDFKTFS
                     RPVIDTLADLGVKAEFTGRNDLVIDGKKICGNAQAYMQGRIMHHGCLLFDTDLTVLSK
                     ALEVPKDKIESKSVKSVRSVVDNILPNLPVKISVNEFSDKLLEHMKSKFPEMKEYTFS
                     EEELADIEKLRASKFGAWEWNYGHSPKFGIERQSRYTAGKIQVFADVKNALIQQIRFY
                     GTFFGNNSNLSEVENALIGVKYTPEDVRKALSNIEFNHFFAGFSLDELTEAIVE"
     gene            complement(1835..2128)
                     /locus_tag="PLI30_13080"
     CDS             complement(1835..2128)
                     /locus_tag="PLI30_13080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007758092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI80525.1"
                     /translation="MKAVMIILDQAHYSQIIDDLSKLNIRGFTSWREVFGRGSKTGEP
                     HYGSHAWPSVNNAVLAVVEDHRVGPLMDYLRELDKAYENLGLRAFVWNVEDMI"
BASE COUNT          576 a          546 c          498 g          634 t
ORIGIN      
        1 gccgtttacg gcatgcacaa catgcccgga ttgccgttgg gtaaatttca tttccgcgaa
       61 ggtgaaacca tggcagcggt ggataactgg gagatttaac tcacgggaaa aggcagccac
      121 ggctccatgc ccgaactgag catcgatccg ctggtatgcg gagcatcgct tgtgatggcc
      181 ttgcaaacca tcgtaagccg caacgtgtct ccgtggaaaa actcggtggt aaacgtggga
      241 gcttttcaat cgggaattgc cggaaacgcc gtaccccaga aagctatttt acggttaagc
      301 atcagaaaca tggaaccagg ccttcgcacg atggtgctcg acaaggtacg cagcatcacc
      361 aaagcgcaag ccgaagcatt taactgcgga tacgaaatcc atgaaggagt ggctggtgcc
      421 gtgcttgtca acactcccga aaacacacaa tgggcttaca aggttgctca ggaaactttt
      481 ggcgaagaga acgctattta cgggatgcac ccgtatatgg gcagcgaaga tttcgctttt
      541 atgctgcaaa agaaagcggg cacttactgc atgatgggta acggcgactc gtttatggtg
      601 catcacccca aatacgtgtt taatcaggac ctgctctcac gcggcgctac gtattgggtg
      661 gcattgacag aagcatattt gtcttgctga tgccgatgca tcgttggttc aaaactctta
      721 ttccacaatc gcttccgtga gttcatccag tgaaaatccg gcaaaaaagt ggttgaactc
      781 tatgttggaa agcgctttgc ggacatcctc cggggtatat tttacaccga taagcgcatt
      841 ttccacttcc gacaggttgc tgttgtttcc aaaaaaagtt ccgtagaatc ggatttgctg
      901 aataagagcg ttcttcacat cggcaaaaac ctgaattttt ccggccgtat accgggattg
      961 cctttcgatg ccaaatttag gagaatgccc gtaattccat tcccaggcac cgaatttcga
     1021 agcacgcaac ttctctatat cagccaactc ttcttccgaa aaagtgtact ctttcatctc
     1081 ggggaattta gatttcatgt gctccagcaa cttatcggaa aattcattga ccgatatctt
     1141 taccggcagg ttgggcaaaa tattatccac tacacttcgt acggacttca cgcttttgct
     1201 ttcgatcttg tctttaggca cttcaagagc ctttgaaaga acggtcaggt cggtatcaaa
     1261 aagcaggcat ccatggtgca tgatccttcc ctgcatgtat gcctgtgcat ttccgcaaat
     1321 ctttttcccg tcgataacca ggtcgtttcg tcccgtaaac tcggccttaa cgcctaaatc
     1381 ggccaaggta tcgatgactg gtctggaaaa tgttttgaaa tcgaaatcgg cacccttatc
     1441 ctcgtttgag ataatggtgt aattgaggtt gttcaggtcg tggtacacag ctcccccacc
     1501 cgatatacgc cgaatgacgt gaatcccgtt ctgttttacg tattccgaat taatctcttc
     1561 tattgtgttc tgatgctttc ccacaataat agccggctcg tttatccaca agataaacac
     1621 tttatcgtgg tgcagcaatt ttttgaagca gtactcttcc agcgctatgt tataatcggg
     1681 tttattggat ttgttgacta tataaatcat tttcttattt tttgtgccac aaagataaaa
     1741 attttatcga agatttggtc gacggctttt caaacaaaac agcagctgct ttttgaaaca
     1801 actgctgttt taaaaactcg gacggtaaac ccatttaaat catatcttcc acgttccaca
     1861 cgaacgcccg aagccccagg ttttcataag ccttgtctaa ctccctcaaa taatccatca
     1921 gcggtcctac gcggtgatct tccaccacag caaggacagc attgtttacc gatggccagg
     1981 catggctgcc ataatgcggc tcgccggttt tacttcctct gccgaatact tcacgccagg
     2041 aggtaaaccc ccggatattg agtttggaaa gatcgtctat tatttgtgaa taatgcgcct
     2101 ggtctaatat tatcattact gctttcatgc ttctgttcct ttcaaataag ttgatgtttc
     2161 tacctgtccg ttttgatggt gaatttcctc acgatgctta cgacgcctgc gtttcacgcc
     2221 gtttcctccg aaaatggagt agagcgcagg aaca
//
LOCUS       DAOSDR010000446         2250 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrimonas sp. isolate AMR_MDS_1826
            NODE_16712_length_2250_cov_2.608200, whole genome shotgun sequence.
ACCESSION   DAOSDR010000446 DAOSDR010000000
VERSION     DAOSDR010000446.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863355
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrimonas sp. (wastewater metagenome)
  ORGANISM  Petrimonas sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Dysgonomonadaceae; Petrimonas.
REFERENCE   1  (bases 1 to 2250)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2250)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:10:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,720
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 35
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2250
                     /organism="Petrimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16712_length_2250_cov_2.608200"
                     /isolate="AMR_MDS_1826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2023866"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            145..765
                     /locus_tag="PLI30_13085"
     CDS             145..765
                     /locus_tag="PLI30_13085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004295452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4141 domain-containing protein"
                     /protein_id="HOI80526.1"
                     /translation="MKRIILCLTVALSIFSFQAKAQWVVTDPTNLVQNIISATQGINT
                     VSNMVKNVNEVKKVYDQGKEYYDALKSVNNLIKDARKVKKTIEMISEITDIYVNGFNK
                     MVGDPSFTPNELVAISAGYAKLLEEGGSLVTELKNVVTGSNGLSLSDKERMEVIDDVY
                     NKMLEYRNLTKYYTQKSISVSFIRAKEKNDTNRVLSLYGTPSERYW"
     gene            785..1771
                     /gene="traJ"
                     /locus_tag="PLI30_13090"
     CDS             785..1771
                     /gene="traJ"
                     /locus_tag="PLI30_13090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006844360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugative transposon protein TraJ"
                     /protein_id="HOI80527.1"
                     /translation="MNFDNLHQILANLYQDMLPLCGSMIGVAKGLAGLGALFYVAYRV
                     WQALSRAEPIDVFPLLRPFAIGLCIMFFPTLVLGTINSVMSPIVKGTNGILESQTMDM
                     KAYQKQKDNLEYQARVREGKAWLVDDEVYDQKMKDLGITDTPEIISMWGERLWYDIKA
                     WFREVMRDFFELLFAASALVIDTIRTFFLIVLAILGPIAFAFSVYDGFQATLTQWLAR
                     YISVYLWLPISDIFSSVLARIQVLMLQQDIVLLQDPNYVPDGSNGVYIVFLIIGIIGY
                     FTIPTVAGWVIQAGGMGSATGAINKTAGKAGAIAGGVAGATAGNVGGRLLGK"
     gene            1818..>2250
                     /gene="traK"
                     /locus_tag="PLI30_13095"
     CDS             1818..>2250
                     /gene="traK"
                     /locus_tag="PLI30_13095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006844359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugative transposon protein TraK"
                     /protein_id="HOI80528.1"
                     /translation="MEFKSLKNIETSFKQIRIIAIVFVVLCAGITGYAVWNSFSFAEA
                     QRQKIYVLDEGKSLMLALSQDLSQNRPVEAKEHIKRFHELFFTLSPDKSAIESNINRA
                     LFLVDKSAFRYYQDMQEKGYYNRIVSNNINQVIQIDSVACNL"
BASE COUNT          689 a          408 c          526 g          627 t
ORIGIN      
        1 tattactttt ttctatttct acggtatcat tcgtattgcc tacgatgatg agcaggatat
       61 gctcgctgac caacgaaggc gaatataaaa gggtgtacca tgaggtataa ctctaaaatc
      121 aataatcaat taaaagtaaa aaatatgaaa cgaataattc tatgcctgac ggtggctctg
      181 tccatattct cgtttcaggc aaaagcacaa tgggttgtaa cagacccgac caatcttgta
      241 cagaatatta ttagtgcaac acagggcatt aacactgtct ctaatatggt caaaaatgtc
      301 aatgaagtaa aaaaggtgta cgatcaaggc aaagaatact acgatgcact taaatcagtc
      361 aataacctga ttaaagatgc ccgtaaagta aaaaagacca tcgaaatgat tagtgagatc
      421 acggacatct atgtgaacgg attcaacaaa atggtaggcg atccgagttt tacgccaaat
      481 gaattggttg ccatttcagc aggctatgcc aaactgttgg aagaaggcgg ttcattagtt
      541 acggaactga aaaatgtagt taccggaagt aacgggctgt ccttgtcgga taaagaacgg
      601 atggaagtaa tagatgatgt atataataag atgttggaat atcgtaatct gaccaaatac
      661 tatacccaaa agagtatctc cgtctcattc attcgtgcga aagagaaaaa cgacacaaac
      721 agagtattgt cactctatgg aaccccgtcc gaaaggtatt ggtaatcaat aaaaataagt
      781 aaagatgaat tttgataatc tacatcagat tcttgcgaat ctgtatcagg atatgcttcc
      841 tctttgcggc agtatgattg gtgtcgcaaa ggggctggca ggactgggag ctttgttcta
      901 tgtggcttac agggtttggc aggcattgag ccgtgccgag cctatcgacg tgtttccgtt
      961 gctacgtccg tttgcaatcg ggttgtgtat catgttcttc ccaactttgg ttttgggaac
     1021 gatcaattca gtgatgagtc cgattgtaaa agggactaac ggtattttgg aatcacagac
     1081 aatggacatg aaagcctatc agaaacaaaa agacaatttg gagtatcaag cacgggtacg
     1141 tgaaggtaag gcatggttgg ttgatgacga ggtatatgac cagaaaatga aagatttggg
     1201 aattaccgat acccctgaaa ttatcagtat gtggggcgaa cgactttggt atgatatcaa
     1261 agcatggttc agggaagtaa tgagagattt cttcgaactg ttgtttgccg cttccgcatt
     1321 agtcatagat acgatacgga catttttcct tattgtgttg gccatactgg ggccgatagc
     1381 atttgccttt tccgtctatg acggattcca ggcaacgctg acccagtggc ttgcacgcta
     1441 tataagtgta tatctatggt tacctatttc cgatattttt tcctctgttc ttgcccggat
     1501 acaagtattg atgcttcaac aggacattgt cctgttacaa gatccgaact atgttcctga
     1561 cggctcaaac ggtgtgtata ttgtgtttct gataatcgga attattggat atttcacaat
     1621 tccgaccgtt gcgggttggg taattcaagc cggaggaatg ggtagtgcta cgggagccat
     1681 taataaaaca gcaggtaaag ctggtgctat tgccggaggt gtagcaggtg caacagcagg
     1741 taatgttggc ggtcgcttac tcggtaaata agcggtgagc caccactggc aataaatgta
     1801 ttaaaaataa aaaaagaatg gaatttaaaa gtttaaaaaa cattgaaaca agtttcaagc
     1861 agatacggat tatcgccatt gtgttcgttg tgctgtgtgc cggaattacg gggtatgcgg
     1921 tatggaactc attcagcttt gcagaagcac aacggcagaa aatctatgta ttggatgaag
     1981 gaaaatcgct tatgctcgct ctctcgcagg acttatcaca aaaccgtcct gtggaagcaa
     2041 aggagcatat caagcgattt catgaactgt tcttcacgct atccccagat aagagcgcca
     2101 ttgaatcaaa catcaacagg gcactctttc tggtggataa aagtgctttc cgctattatc
     2161 aggacatgca ggagaaaggg tactacaata ggattgtgtc gaataatatc aatcaggtta
     2221 ttcagattga ttctgttgcc tgtaatctcg
//
LOCUS       DAOSDR010000447         2249 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrimonas sp. isolate AMR_MDS_1826
            NODE_16716_length_2249_cov_3.407019, whole genome shotgun sequence.
ACCESSION   DAOSDR010000447 DAOSDR010000000
VERSION     DAOSDR010000447.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863355
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrimonas sp. (wastewater metagenome)
  ORGANISM  Petrimonas sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Dysgonomonadaceae; Petrimonas.
REFERENCE   1  (bases 1 to 2249)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2249)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:10:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,720
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 35
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2249
                     /organism="Petrimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16716_length_2249_cov_3.407019"
                     /isolate="AMR_MDS_1826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2023866"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            172..1755
                     /locus_tag="PLI30_13100"
     CDS             172..1755
                     /locus_tag="PLI30_13100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019540831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HOI80529.1"
                     /translation="MNKILSSLVALFSVTALCAQDTPIVPKLVVGITIDQLRGDYLEM
                     FQHTFGEKGFKKLLNGGLVYADLEFDFPHLDRASTITTIFTGANPAYHGITGENRFIL
                     KENREIPAFSDNNYAGNFTTERYSPLPVKVSTLADELKIASGGQSDVFAFAPHASQAL
                     ASGGHAASGAYWVEDMTGNWATTAFYKTRQPIVDQHNRTPESLSKKIGSITWRPAIDI
                     SNYKAFPYTKNLYNFQHYFGVDKKNHVRLLKQSPYVNTEVREIAEKVLKSSSLGKRQN
                     PDFLALTFYAGNFENSLDKNYSVEVQDTYYRLDQEIEKLLEAIDSAVGLQNTLIFVVS
                     TGYFNEQEIHPSDMVTSGGDFYPDRSIALLNMYLMAIYGREQWVSKYYSQQLFFNRKL
                     IEDRKINFVEFQQKAAEFLVQSAGIQDVITSHQMLHGAYNQRVQHYRNGFYKGISGDL
                     YLELQPGWKVVVENDLNNNVRVRDNAVHAPVIFFGNNIKPQRINRVIDATEIAPSVSH
                     RLRIRAPNAAQANILEELF"
     gene            1853..>2249
                     /gene="secA"
                     /locus_tag="PLI30_13105"
     CDS             1853..>2249
                     /gene="secA"
                     /locus_tag="PLI30_13105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007758327.1"
                     /note="functions in protein export; can interact with
                     acidic membrane phospholipids and the SecYEG protein
                     complex; binds to preproteins; binds to ATP and undergoes
                     a conformational change to promote membrane insertion of
                     SecA/bound preprotein; ATP hydrolysis appears to drive
                     release of the preprotein from SecA and deinsertion of
                     SecA from the membrane; additional proteins SecD/F/YajC
                     aid SecA recycling; exists in an equilibrium between
                     monomers and dimers; may possibly form higher order
                     oligomers; in some organisms, there are paralogous
                     proteins that have been found to be nonessential but do
                     function in secretion of a subset of exported proteins;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HOI80530.1"
                     /translation="MGFNSFISKIFGNKAQRDLNEINPIVNLIKEAYPGIAKLSNDEL
                     RAKTKELEKKIEEYITGDNARIAELKASIEEVELEKRESVWNEIDKIEKEITSKLGKI
                     LLEILPEAFSIVKETAKRFKENPETVVTAT"
BASE COUNT          705 a          485 c          499 g          560 t
ORIGIN      
        1 cgaaaaggag aaaggttaat aagcggtatg tttcgtcgaa atacatgaag gcaggttggg
       61 ggcaatgatg tgaatcaaaa cgtagcacgg tttttgcatg atacaataag aactggattg
      121 cgcacataaa gaagccaaca gaacaagctt aaatgaaaga tatactctca aatgaataaa
      181 atattatcct ctcttgtcgc tcttttttcg gtaaccgctc tttgtgccca ggacacacca
      241 atcgtcccca agttggttgt ggggatcacc atagatcaat tacgcggcga ttacctggag
      301 atgtttcaac atactttcgg agaaaaagga tttaaaaagc tcctgaatgg ggggttggtt
      361 tatgcagatc tggaatttga ttttccccac ctcgatcgtg cttccaccat cactaccatt
      421 ttcacgggtg ccaatcccgc ttaccacgga attaccggag aaaacaggtt tatactgaaa
      481 gaaaaccggg aaattcctgc tttttccgat aacaattatg ccggaaattt cactaccgaa
      541 cgttattctc cattacctgt aaaggtttct acccttgccg acgaactcaa aatcgcttcc
      601 ggaggacaat cggatgtgtt tgcttttgcg cctcacgctt cacaggcatt ggcatccgga
      661 ggacacgctg ccagtggtgc gtactgggtt gaagacatga ccggaaactg ggcaacgacg
      721 gctttttata aaacccggca acccatcgtg gatcaacata accgtacacc ggagtcattg
      781 agtaagaaaa ttggttccat cacgtggcgc ccagctatcg acatatcgaa ttacaaagcg
      841 tttccttata ccaagaacct gtataacttc cagcattact tcggtgtgga taaaaaaaat
      901 cacgttcggt tattgaaaca gtcgccttat gttaacacgg aagttcgtga aattgcagag
      961 aaggtactaa aatccagctc gttgggaaaa cgtcaaaatc ctgatttcct ggcgttgact
     1021 ttctatgccg gcaattttga aaattcgctc gacaaaaact attccgttga agtgcaagac
     1081 acttactacc ggctggatca ggaaatagag aaactgctcg aagctattga ttcagctgta
     1141 ggcctgcaga acaccctgat ctttgtggtt tccaccggat atttcaacga acaggagatc
     1201 cacccttcgg atatggttac ttcgggaggt gatttttatc ccgacaggag catcgcattg
     1261 ctcaacatgt atctcatggc tatttacggc cgcgaacaat gggtcagtaa atattacagc
     1321 cagcagctgt ttttcaatcg caaacttatc gaagaccgga aaatcaactt cgtggaattt
     1381 cagcaaaaag cggctgaatt tcttgttcag tcggccggta tacaggatgt cattacctct
     1441 caccagatgc tgcacggtgc ttacaatcaa cgtgtgcaac attacagaaa tggtttctac
     1501 aaaggaatat cgggtgatct ttacctggaa ttacagcccg gatggaaggt agtggttgag
     1561 aatgacctca acaacaacgt ccgcgtaaga gacaatgccg ttcatgcgcc ggtcatcttt
     1621 ttcggaaaca acattaaacc gcagcggatc aatcgtgtga ttgatgcaac ggagatcgcc
     1681 cccagcgttt cacaccgctt gcggattcgt gctcccaacg ctgcccaagc caatattttg
     1741 gaagaactgt tttaaataga gaagttaaca attaacaatt aaaattaagg ggaccggata
     1801 aatccgtaca acaggtcgga cctgaaatcc gaaacccgca atacaaaaca atatgggatt
     1861 caattcattt ataagtaaaa tatttggtaa taaggcacag agggacctga acgagataaa
     1921 ccccattgta aatttaataa aggaggctta tccgggaata gccaagctct caaacgatga
     1981 gttacgtgca aaaaccaaag aactcgaaaa gaaaatagaa gaatacatta ccggggataa
     2041 cgcccgtatc gcggagctaa aagccagtat tgaagaagtg gaactcgaaa aacgcgagtc
     2101 ggtttggaac gagatagata aaattgaaaa ggagattaca tcgaaattgg ggaaaatatt
     2161 gttggaaatt cttcccgaag cattttctat tgtcaaggaa acagctaaaa gatttaagga
     2221 aaacccggaa actgtggtta cggccaccg
//
LOCUS       DAOSDR010000448         2188 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrimonas sp. isolate AMR_MDS_1826
            NODE_17286_length_2188_cov_3.459916, whole genome shotgun sequence.
ACCESSION   DAOSDR010000448 DAOSDR010000000
VERSION     DAOSDR010000448.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863355
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrimonas sp. (wastewater metagenome)
  ORGANISM  Petrimonas sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Dysgonomonadaceae; Petrimonas.
REFERENCE   1  (bases 1 to 2188)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2188)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:10:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,720
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 35
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2188
                     /organism="Petrimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17286_length_2188_cov_3.459916"
                     /isolate="AMR_MDS_1826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2023866"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..471)
                     /locus_tag="PLI30_13110"
     CDS             complement(<1..471)
                     /locus_tag="PLI30_13110"
                     /inference="COORDINATES: protein motif:HMM:NF027445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4835 family protein"
                     /protein_id="HOI80531.1"
                     /translation="MKIISKKGATVRHKFMKNAVFSVIFWFIAVGCTMAQNLNATVSV
                     NSSRIQGNRQLFNTLEEQLRIFINDGKWTDTHPPAHGKIDCSFTLVVNEMSSPSSFKG
                     ELQVQARRPVPGASYKTPLLNYREPSFLFDYMEYQSLEFNPDNIPNNLVATIAFY"
     gene            complement(472..765)
                     /locus_tag="PLI30_13115"
     CDS             complement(472..765)
                     /locus_tag="PLI30_13115"
                     /inference="COORDINATES: protein motif:HMM:NF024979.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone modulator CbpM"
                     /protein_id="HOI80532.1"
                     /translation="MNEKRILYSECIRIYEVEGSFLDSLGELGLIRLLDSGDSRFVEY
                     DELEELEQFVRWHNEMDINAEGIEALYYMLVRVKLLQDEIDSLRNELQFYRSM"
     gene            complement(762..1685)
                     /locus_tag="PLI30_13120"
     CDS             complement(762..1685)
                     /locus_tag="PLI30_13120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010800047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HOI80533.1"
                     /translation="MAYIDYYNILGVSKNASADDIKKAYRQKARKLHPDLNPNDKEAH
                     RKFQQLNEANEVLSDPEKRAKYDKYGENWKHGEEYEKAQQQYQQQQHQWGGQQGEQTF
                     YTEGDFSDTDFSEFFHSMFGGGFTQRETRRTNRKYKGGDYQAELRLSLRQAMYTHQQT
                     LNLDGKNIRITIPAGVTDGQKIKLAGYGQPGVNGGPNGDLYITFVIEDDPEFKRLGND
                     LYTSVHVGLYTAVLGGEMTVNTLDGQVKMKVKPGTQPEAKVRLKGKGFPVYKKDDQFG
                     DLIVTMKVEVPKNLSSKEQELFVELSKLNQR"
     gene            complement(1761..>2188)
                     /locus_tag="PLI30_13125"
     CDS             complement(1761..>2188)
                     /locus_tag="PLI30_13125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008772625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HOI80534.1"
                     /translation="LENIFFDFDKAELRPESKKELDGLIGLLNDNPSVTIELSAHTDR
                     KGAEGYNQDLSLRRAQSVVRYLIGKGVDERRLSAAGFGKTQPKTVSDTIAEQYGFLKE
                     GDRLDEAFIEKLTPEQQEIADQINRRTEFRVTGSSFGLF"
BASE COUNT          550 a          549 c          426 g          663 t
ORIGIN      
        1 ataaaaggcg atggtggcaa ccaggttgtt gggaatattg tcgggattga actccaacga
       61 ctgatattcc atgtaatcga acaaaaagga aggctcacga taattcagta acggcgtctt
      121 gtatgatgca ccgggaacag gccttcgtgc ctgaacctgc agctctccct taaacgacga
      181 aggagatgac atctcattga cgacgagggt aaacgaacaa tcaatctttc catgtgccgg
      241 aggatgcgta tccgtccatt ttccatcgtt tataaatatc ctcaactgct cttcgagtgt
      301 attaaacaac tgccggtttc cctgtatccg ggaactgttg acagagacag tggcattgag
      361 attctgggcc atcgtacaac caacagctat aaaccagaaa ataacagaaa aaacggcatt
      421 cttcataaac ttatgcctga cggtggctcc tttttttgat attattttca attacatcga
      481 acggtaaaac tgcaattcgt tccgcaaact atctatttca tcttgaagca atttcacccg
      541 tacaagcata taatacagcg cctcaatacc ttccgcgtta atatccatct cattgtgcca
      601 gcggacaaat tgttccagtt cttccaactc gtcgtattcc acaaaccggg agtcaccgga
      661 atccaacaag cgaattaacc ccagttcgcc cagtgaatcg agaaacgatc cttctacttc
      721 ataaatacgg atgcattcgc tgtataaaat tcttttttcg ttcatcgttg gttcagttta
      781 gataattcga caaacaactc ctgctctttt gacgagaggt ttttgggtac ctctactttc
      841 attgtcacga taaggtcgcc gaactgatca tcttttttat atacaggaaa acctttgcct
      901 ttcaagcgaa ccttcgcttc aggctgtgtg ccaggcttta ctttcatttt cacctgtccg
      961 tcaagcgtat ttacggtcat ttctccaccg agaacggccg tgtataaccc aacatgaacc
     1021 gacgtgtata gatcgttgcc aagccgcttg aattccgggt cgtcttcaat aacgaacgta
     1081 atgtataaat ctccgttagg cccaccgttt actcccggct ggccatatcc ggcaagcttt
     1141 atcttttgtc catctgtaac ccctgcagga atagttatac gtatattttt cccgtcaagg
     1201 ttaagcgttt gctgatgggt atacatggct tggcggagcg ataaccgcaa ctctgcctga
     1261 tagtctcccc ccttgtactt tctattcgta cgcctggttt ccctttgtgt aaaaccaccg
     1321 ccaaacatcg aatgaaaaaa ctcagaaaaa tcggtatccg aaaaatcacc ttcggtgtag
     1381 aaagtttgct ccccctgttg tccaccccat tgatgctgtt gctgttgata ttgctgctgc
     1441 gctttttcgt attcctcgcc gtgtttccag ttttctccgt atttatcgta ttttgcccgt
     1501 ttttcagggt cgctcaacac ttcgttcgct tcgttgagtt gctgaaattt cctatgcgcc
     1561 tccttatcgt ttggattcaa atccggatgc agcttacgcg ccttttgccg gtaggctttt
     1621 ttaatgtcat cggccgacgc atttttactt acgcccaaaa tattgtagta atcaatatat
     1681 gccattttta aaattattat gatacaaata tagcaaaaaa tgcgccttta ttactgctcc
     1741 ttcacacgaa aatataacga tcaaaataat ccaaaggatg agccggtcac cctgaattct
     1801 gtccgccggt taatctggtc tgcaatctcc tgttgctcgg gtgtcagctt ttcgataaat
     1861 gcctcatcca gcctatcccc ttctttcagg aaaccgtatt gttccgcgat ggtatcagaa
     1921 accgttttag gttgtgtttt cccaaaacct gccgccgata atcgccgctc atccactccc
     1981 ttgccgataa ggtaccgtac cactgactgt gcgcggcgta acgaaaggtc ctgattgtat
     2041 ccttcggccc ctttcctgtc ggtatgcgcg gaaagttcga tggttactga cggattgtca
     2101 tttaacaatc ctataagtcc gtccagttct ttttttgact cgggacgcaa ctccgcttta
     2161 tcaaaatcga agaatatatt ttcgagga
//
LOCUS       DAOSDR010000449         2188 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrimonas sp. isolate AMR_MDS_1826
            NODE_17289_length_2188_cov_3.044069, whole genome shotgun sequence.
ACCESSION   DAOSDR010000449 DAOSDR010000000
VERSION     DAOSDR010000449.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863355
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrimonas sp. (wastewater metagenome)
  ORGANISM  Petrimonas sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Dysgonomonadaceae; Petrimonas.
REFERENCE   1  (bases 1 to 2188)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2188)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:10:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,720
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 35
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2188
                     /organism="Petrimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17289_length_2188_cov_3.044069"
                     /isolate="AMR_MDS_1826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2023866"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(145..1194)
                     /locus_tag="PLI30_13130"
     CDS             complement(145..1194)
                     /locus_tag="PLI30_13130"
                     /inference="COORDINATES: protein motif:HMM:NF024729.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integrase core domain-containing protein"
                     /protein_id="HOI80535.1"
                     /translation="MLIEEHYPLTGKPYPKRLIFKVVGYSSSTWYENPTPKTGKRGRK
                     PKHSDEEVLQEIKEEIKKSTFNAEGYLKVKKRMGKRKINALVAGKARVNRIMRENNLL
                     SPYRRPGKTNKREHDGTIITDAPNVMWATDGKRFWIDGSGWHWFFGVIDHFNDEIISW
                     HIARKGNRFAAMEPVRAAVRKTFGSVGKDVCKGMKLQLRSDHGSQYDSADFMNEMKFL
                     GLEVSKAFVRSPQCNGIIERFHRTLEEQVLQTETFSSFEEAYNSINQFINDYNTDWIF
                     HRLEYCSPVEYREKYAESQRKENDDIPSGNKDPEVQLVLISSGSMPRRNEIALQSMIK
                     GAITYSNTPSINPSV"
     gene            complement(1200..1481)
                     /locus_tag="PLI30_13135"
     CDS             complement(1200..1481)
                     /locus_tag="PLI30_13135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI80536.1"
                     /translation="MEKRKNFTSKIKAELVLSLLRGEDPELLSREYGVTLADINLWRD
                     QFIESGTDGFKRKPDDSRLGAAERKIGQLQMELELTKKKNELAAKLKRK"
     gene            1719..>2188
                     /locus_tag="PLI30_13140"
     CDS             1719..>2188
                     /locus_tag="PLI30_13140"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HOI80537.1"
                     /translation="MNQLINYIKTYVALSDEECEVLMSLFEIRKLRNNEVFFEEGQIA
                     NELYFVTKGCIRLFYKVDVDEKTAFFYSQGQFICAGESYNLGIAAKENHQSMGQSEIV
                     ILDKKKVETLLQKDSKFEAIGRIATENELIASQKIIASFVTQTAEERYLELLES"
BASE COUNT          607 a          467 c          422 g          692 t
ORIGIN      
        1 tgaataccgc caaacgaatt ataatcgggt tacggtgtaa ccgataacaa agtgctgtat
       61 tagcccgtaa taaccgagca cattaattgt atttaatctt ttttattact tttgtatcgg
      121 acaagctgat ccgatggtga tatattatac cgaaggattt attgaagggg tgttactgta
      181 agtaattgcc ccttttatca ttgactgaag agctatttca tttcttcgtg gcattgagcc
      241 acttgaaatg aggacaagct gaacctcagg atccttattg cccgatggta tgtcatcatt
      301 ttcttttcgc tggctttcgg cgtacttttc cctgtattct acaggagaac agtattccag
      361 tctatgaaat atccaatccg tgttgtagtc attaataaat tgattaatac tgttataagc
      421 ttcctcaaag gaagaaaatg tttctgtttg caacacctgt tcttccaggg tgcggtgaaa
      481 ccgctctatt atcccgttgc attgcggtga ccgtacaaac gctttggaca cctccaatcc
      541 caggaatttc atttcattca taaaatcagc agagtcatac tgcgagccat ggtcgcttct
      601 taattgcaac ttcattcctt tacacacatc cttacctacc gaaccgaaag tcttccgtac
      661 agcggcccta acaggctcca tggcggcaaa ccggttgcct ttccttgcaa tatgccatga
      721 gataatctca tcgttaaagt gatcgatcac accaaagaac caatgccaac ctgacccatc
      781 aatccaaaac ctcttcccgt cagtggccca cattacattg ggtgcatccg tgattatagt
      841 accatcatgt tcgcgcttat tcgtcttccc gggccttctg taggggctca gcaggttgtt
      901 ttcccgcatg atgcggttca cacgtgcctt gccggctacc agagcgttta tcttcctttt
      961 acccatgcgc ttcttcactt tcagataacc ttcagcgttg aatgtactct tcttaatttc
     1021 ttccttaatc tcctgtaaaa cctcttcatc gctatgcttg ggttttctgc cccgtttccc
     1081 tgttttggga gtagggtttt cataccaggt gctgctgctg tagccgacta ccttgaatat
     1141 caaacgcttc ggatatggtt tcccggttaa tggataatgt tcctctatca gcatggagat
     1201 tatttccttt ttagttttgc tgccaattcg ttcttttttt ttgtgagttc gagctccatc
     1261 tgcagttgcc cgatcttacg ctcggcagca cccagcctcg aatcgtcggg tttacgtttg
     1321 aacccgtcgg taccactctc gataaattga tcccgccaaa ggttgatatc agccagagta
     1381 actccgtatt cacggctaag cagctccgga tcctctccac gcaacaagga taaaactaat
     1441 tcagccttga ttttcgatgt aaaatttttc ctcttttcca tagcagttgt aaaaataact
     1501 tttatttttt actgctcggt ttatttgagg gctaatataa attttaaaat caactatcct
     1561 aaatcgttat cttagaaaat ccgttcatac ctattcctga tgaactaaac accgaacaag
     1621 aagatgacat agtgggattg tggagctggg atgggaaagg aaaagttgtt gtaaattgta
     1681 catttcataa taaatgattg ctaaattaga tacaccatat gaatcaacta ataaactata
     1741 tcaaaaccta tgtcgcatta tcagatgaag agtgcgaagt gctcatgtct ttatttgaaa
     1801 taaggaaact gcgaaataat gaggtgtttt ttgaagaagg gcaaatagca aatgaactct
     1861 attttgtaac gaaaggctgt atccgattat tttacaaagt ggatgtggat gagaaaacag
     1921 cttttttcta ttcgcaaggg caatttatat gtgctggaga aagctataat ctaggtattg
     1981 ctgctaaaga gaatcatcag tctatgggtc aatctgagat agttatattg gataagaaga
     2041 aggtagaaac tttgctccaa aaagattcca agtttgaagc aataggacgt atagctaccg
     2101 aaaatgaatt gattgccagt caaaagataa ttgcatcttt tgttactcaa acagccgaag
     2161 agcgatacct tgaattatta gaaagcaa
//
LOCUS       DAOSDR010000450         2184 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrimonas sp. isolate AMR_MDS_1826
            NODE_17342_length_2184_cov_4.851574, whole genome shotgun sequence.
ACCESSION   DAOSDR010000450 DAOSDR010000000
VERSION     DAOSDR010000450.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863355
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrimonas sp. (wastewater metagenome)
  ORGANISM  Petrimonas sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Dysgonomonadaceae; Petrimonas.
REFERENCE   1  (bases 1 to 2184)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2184)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:10:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,720
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 35
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2184
                     /organism="Petrimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17342_length_2184_cov_4.851574"
                     /isolate="AMR_MDS_1826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2023866"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1169)
                     /locus_tag="PLI30_13145"
     CDS             complement(<1..1169)
                     /locus_tag="PLI30_13145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009275823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HOI80538.1"
                     /translation="MVNNNKSYEKRKYIIASVVVVTLLVYIAQLFNLQILSPEYRNFA
                     DSNAFFRKTLYPARGAIYDRNGELVVFNQPTYDVMMVVREMKQFDTLDFCNTLNIDKA
                     AFLQLEAEMKDRRKNPGYSSYTPQLFMSQLDVEEYGLLQEKLYKFPGIYIQNRTERQY
                     KYPNMGLILGYVGIVDKNKMDSDPYYTRTDYVGKSGIELSHEEDLRGEKGVEVLLRDA
                     HGRVQGKYRDGEFDKSPVSGRDLTLAIDINLQAYGEYLMQNKIGSIIMIEPKTGEILC
                     MVAAPSYDPSILTGKNFSQNYLQLEQDPYKPLINRAVSGLYPPGSTFKPSQGLIFLEE
                     GIITPDTRYSCFGGYPPLGGRPACHGHASPLSLQPALATSCNSFFCYGLNAMLNNR"
     gene            complement(1162..1677)
                     /gene="mreD"
                     /locus_tag="PLI30_13150"
     CDS             complement(1162..1677)
                     /gene="mreD"
                     /locus_tag="PLI30_13150"
                     /inference="COORDINATES: protein motif:HMM:TIGR03426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HOI80539.1"
                     /translation="MKAVSIFKEILLFVILVLLQVLLLNRIAVFGLAVPILYIYFLIK
                     LPYGRNRFYVIVSGFLLGIVIDIFLNTPGMNAAATTIIATLRSMVLNLFYPKNEYEDL
                     VPGIHTSTAAFVKFTVMMVLLHQTLLFFIEAFTLFNLTSTLIRIGSSSILTIVLILAL
                     DSLSIRREKSG"
     gene            complement(1677..>2184)
                     /gene="mreC"
                     /locus_tag="PLI30_13155"
     CDS             complement(1677..>2184)
                     /gene="mreC"
                     /locus_tag="PLI30_13155"
                     /inference="COORDINATES: protein motif:HMM:NF016011.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005618 - cell wall [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HOI80540.1"
                     /translation="FVPAEVINVSFSGPNNFITVNKGSIHGVKPDMGVVSQNGVVGVV
                     LKTSPSFSVVIPIINPKFRLSAKLKNSNNTGSISWDGKDLITAQIGELPKHEVFQPGD
                     TVVTSFSRIFPKDIVIGYVTGQILSKDDNFNTLNLRIATDFYSVRDILIINDAHFDEQ
                     NELENSIP"
BASE COUNT          633 a          476 c          458 g          617 t
ORIGIN      
        1 cggttattaa gcatggcgtt aagtccataa cagaaaaacg agttgcacga agtggccagt
       61 gccggctgca ggctgagcgg tgaagcgtgt ccgtggcagg caggacgccc tcccaatggc
      121 ggatagcctc cgaaacagct gtaccgggta tcaggagtaa tgattccttc ttccagaaaa
      181 atcaatcctt gtgagggctt aaaagtggat cctggaggat acaatcccga aacagcccgg
      241 ttgatcaacg gcttgtaagg atcctgttcg agttgaaggt aattctggct gaagtttttt
      301 ccggtcagga tcgacggatc ataagaaggt gcagccacca tacaaaggat ttcgcctgtt
      361 ttgggttcaa tcataataat ggacccgatt ttgttctgca tcaggtactc cccgtacgct
      421 tgcaaattaa tgtcaatggc cagcgttaaa tcacgacctg aaacgggtga tttgtcaaat
      481 tctccgtcgc ggtattttcc ctgtacgcgc ccgtgtgcgt cgcgtaaaag gacttctact
      541 cctttctctc cccgcaaatc ctcttcgtga gaaagttcga tccccgattt ccccacgtaa
      601 tcagtacgtg tataatacgg gtcggaatcc atcttgtttt tgtcgactat gcccacgtac
      661 cccaatatca accccatatt aggatatttg tattgtcgct cggtccggtt ttgaatataa
      721 attccgggga acttgtacaa tttctcctgc aacaagccgt attcttccac gtcgagttgc
      781 gacatgaaaa gctgcggtgt ataggaggaa tagccgggat tttttcgccg gtctttcatt
      841 tcggcttcca gctgtagaaa agctgctttg tcgatgttta gcgtattgca aaaatcgagc
      901 gtgtcaaatt gcttcatttc acggacaacc atcatgacat cgtatgtggg ctgattgaaa
      961 acgaccaatt cgccgtttct gtcataaatg gctccacgtg ccggataaag tgtttttctg
     1021 aaaaaagcgt tgctgtcggc aaagtttcga tattccggac taagaatctg caaattaaat
     1081 agttgtgcaa tatacaccaa tagcgttacc acgacaacag acgcaatgat atacttcctt
     1141 ttctcgtatg acttgttatt gttaaccact tttctctctc ctgattgata gggaatccag
     1201 tgccagaatc aggacaatag tcagtatcga agaagaccca atccggataa gggtgctggt
     1261 taaattaaag agtgtgaagg cttcgataaa aaagagcaac gtctgatgca acaataccat
     1321 cattactgta aatttcacaa aagccgctgt tgacgtatga ataccgggaa ccaggtcttc
     1381 gtactcattt ttgggataaa acaggtttaa taccatgctt ctcagtgtag caataatggt
     1441 tgtggcagca gcattcatac cgggtgtatt caaaaaaata tcgataacaa ttcccaacag
     1501 gaaacccgaa acaattacat agaaccggtt tcttccgtaa ggcagtttta tgagaaaata
     1561 gatatacaga ataggcacag ccaatccaaa aacagcaata cgattaagca gcaatacttg
     1621 taacaatacc aatattacga acagcaaaat ctctttgaat atggaaaccg ctttcattat
     1681 ggaattgaat tttctaattc attctgttcg tcaaaatgcg catcatttat aataagaata
     1741 tccctcacag aataaaaatc agtagctatt cgcaaattta acgtattgaa attgtcatct
     1801 ttagataaaa tctgcccggt aacgtatccg ataacaatat cttttgggaa aatgcgtgaa
     1861 aaactggtta ctaccgtgtc tcccggttga aaaacttcgt gtttgggaag ttctcctatc
     1921 tgcgctgtaa tcaaatcctt gccgtcccac gaaattgatc ccgtattgtt ggaatttttc
     1981 agtttggcac tcaacctgaa tttcggattt ataatgggaa taaccactga aaatgatgga
     2041 gaggtcttca atactactcc caccactcca ttttgtgaaa ctacacccat atcaggtttg
     2101 acaccgtgta tggaaccttt gtttaccgtg atgaaattgt ttgggcccga gaaactgaca
     2161 ttgatcactt cagcgggaac aaat
//
LOCUS       DAOSDR010000451         2168 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrimonas sp. isolate AMR_MDS_1826
            NODE_17524_length_2168_cov_2.630857, whole genome shotgun sequence.
ACCESSION   DAOSDR010000451 DAOSDR010000000
VERSION     DAOSDR010000451.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863355
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrimonas sp. (wastewater metagenome)
  ORGANISM  Petrimonas sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Dysgonomonadaceae; Petrimonas.
REFERENCE   1  (bases 1 to 2168)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2168)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:10:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,720
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 35
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2168
                     /organism="Petrimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17524_length_2168_cov_2.630857"
                     /isolate="AMR_MDS_1826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2023866"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            165..1043
                     /gene="rfbA"
                     /locus_tag="PLI30_13160"
     CDS             165..1043
                     /gene="rfbA"
                     /locus_tag="PLI30_13160"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007657099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HOI80541.1"
                     /translation="MKGIVLAGGSGTRLYPITKGISKQLIPVYDKPMVYYPISALMLA
                     GIKDILIISTPHDLPGFERLLGDGSDYGVTFSYAEQPSPDGLAQAFIIGEEFIGDDCA
                     CLVLGDNIFYGQGFPNLLRQAVKDAESGNNATVFGYWVNDPERYGVAEFDPEGNVLSI
                     EEKPQQPKSNYAIVGLYFYPNKVVEVAKSIRPSARGELEITAVNQQFLKDRQLKVQLF
                     GRGFAWLDTGTFGSLSEASTFIEVLEKRQGLKISCLEEIAWRNGWINDEKLRHLAEPM
                     KKNEYGRYLLKLIGEA"
     gene            1043..1585
                     /gene="rfbC"
                     /locus_tag="PLI30_13165"
     CDS             1043..1585
                     /gene="rfbC"
                     /locus_tag="PLI30_13165"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HOI80542.1"
                     /translation="MKITTTAIEGFVILEPEVFGDERGYFFESFSQREFEEKVCKTTF
                     VQDNESSSRYGVLRGLHFQKPPHAQAKLVRVVKGKVLDIAVDIRKGSPTFGHHVSIEL
                     SGENKRQLFIPRGFAHGFAVLSEEVVFQYKCDNYYVPHSEGGILWNDPALGIDWKLPE
                     EDVILSEKDKKNVLLNELKL"
     gene            1588..>2168
                     /locus_tag="PLI30_13170"
     CDS             1588..>2168
                     /locus_tag="PLI30_13170"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016270226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HOI80543.1"
                     /translation="MEGQFKRNILITGGAGFIGSHVVRLMATNYPHYRIVNLDKLTYA
                     GNLANLLDVRDKPNYRFVKADICDFDAVLRVFEAYAIDSVIHLAAESHVDRSITDPFS
                     FAQTNVMGTLNLLEAARRMWQPQTSHLFYHVSTDEVYGALSFDDTLFTEETRYDPHSP
                     YSASKAASDHFVRAYHDTYGLPVVISNCSNNYGP"
BASE COUNT          535 a          583 c          551 g          499 t
ORIGIN      
        1 tttaaaaaaa ttacaacatt tgtcgtttaa ttttgttatt tttgtaattg ttatgctcat
       61 taataatttg catgtgcttt aattgtagct aaatcttcga ctgacaatct ttagctttta
      121 ttaagagcga agcgaaacga aagtaatttt cttaaacaag atatatgaaa ggaatcgtac
      181 ttgccggagg gtccggcacc cgcctctatc cgatcacgaa aggaatctcc aagcaactta
      241 tcccggtcta cgataaacct atggtctatt atcccatctc cgcccttatg ctcgcaggga
      301 tcaaggatat cctcatcatc tccacgccgc acgaccttcc cgggtttgaa cggttgctgg
      361 gcgacgggtc ggactacggc gtcacctttt cctacgcgga acaacccagc cccgacgggc
      421 tggcccaggc gttcatcatc ggcgaggagt tcattggcga cgattgcgcc tgcctggtgc
      481 tgggcgacaa tattttttac gggcagggtt tccccaatct gttgaggcag gccgtcaagg
      541 atgcggaatc gggcaacaac gccactgtct tcggatactg ggtgaacgac ccggaaaggt
      601 acggggtggc cgaatttgac ccggagggca acgtgctgag catcgaggag aaaccccaac
      661 agcccaaatc gaattacgcg attgtgggcc tctatttcta cccgaacaag gtggtggaag
      721 tggccaaaag cattcgtccc tccgcacgag gagagttgga gataaccgcc gtgaaccagc
      781 agtttctgaa ggatcgacag cttaaggtcc aactctttgg ccgtggcttc gcctggctcg
      841 acaccggcac cttcggctcc ttgtctgaag cgtccacatt tatcgaagtg ctggagaaac
      901 ggcaggggct gaagatatcc tgcctcgaag agattgcctg gcgcaacggc tggatcaacg
      961 acgaaaagct ccggcacctg gcggaaccga tgaaaaaaaa cgaatacggc cggtatctgc
     1021 taaaactaat cggggaggca tgatgaagat caccaccaca gccattgaag gctttgttat
     1081 tcttgagccg gaagtgttcg gcgacgagcg gggctatttc ttcgaatcgt tttcccagcg
     1141 ggagttcgaa gaaaaagtat gcaagaccac ctttgtccag gacaacgagt ccagctcccg
     1201 ctacggtgtc ctgcggggat tgcatttcca gaaaccgccc cacgcgcagg ccaagctggt
     1261 gcgtgtggta aaagggaaag tgctggacat tgccgtggat atccggaaag ggtcgccaac
     1321 atttgggcat cacgtctcga tagaattatc cggtgagaac aagcgtcagc tcttcattcc
     1381 ccggggcttt gcccacggtt tcgcggtgct gagcgaagag gttgtttttc agtacaaatg
     1441 cgacaattac tacgttcccc attccgaagg cggtatcctc tggaacgacc ctgcgttggg
     1501 gatcgattgg aaattaccgg aagaagatgt aattttgtcg gagaaagaca aaaaaaatgt
     1561 gttattaaac gagttaaaat tatagtgatg gaaggccaat tcaaaagaaa tatactgatt
     1621 accggcggag ccggatttat cgggtcgcac gtggtgaggt taatggcaac caactatccc
     1681 cattaccgca tcgtgaacct cgacaagctt acctatgccg gcaacctcgc caacctgctc
     1741 gatgtccggg acaaacccaa ttaccggttc gtcaaagccg atatctgcga cttcgatgcc
     1801 gtgctacgcg tgtttgaagc gtatgccatc gacagcgtta tccacctggc ggcagagagc
     1861 catgtcgacc gctccattac cgatccgttt tcgtttgctc aaacgaacgt catgggcacc
     1921 ctcaacctgc tggaagcagc ccgccggatg tggcaacccc aaacctcaca cctcttctac
     1981 cacgtttcca ctgacgaggt gtacggtgcg ctgtctttcg acgacaccct tttcaccgaa
     2041 gagacccgct acgacccgca ttcaccctac tccgcctcca aggcggcttc cgaccacttc
     2101 gtgcgggcct atcacgacac ctacggactg cccgttgtta tatccaactg ctccaacaac
     2161 tacgggcc
//
LOCUS       DAOSDR010000452         2160 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrimonas sp. isolate AMR_MDS_1826
            NODE_17602_length_2160_cov_4.083135, whole genome shotgun sequence.
ACCESSION   DAOSDR010000452 DAOSDR010000000
VERSION     DAOSDR010000452.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863355
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrimonas sp. (wastewater metagenome)
  ORGANISM  Petrimonas sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Dysgonomonadaceae; Petrimonas.
REFERENCE   1  (bases 1 to 2160)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2160)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:10:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,720
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 35
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2160
                     /organism="Petrimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17602_length_2160_cov_4.083135"
                     /isolate="AMR_MDS_1826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2023866"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..312
                     /locus_tag="PLI30_13175"
     CDS             <1..312
                     /locus_tag="PLI30_13175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007902941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOI80544.1"
                     /translation="VIFIGGMTGLYCLVATSVFMSLMFPTIYGIALKGLGDDSTLGAA
                     GLVMAIVGGALMPPLQGAIIDLGTIAWLPAVNASFVLPFICFTVICIYGLRTNRRRIL
                     G"
     gene            478..1890
                     /locus_tag="PLI30_13180"
     CDS             478..1890
                     /locus_tag="PLI30_13180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010536848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOI80545.1"
                     /translation="MKKLIYLSIITTVLFASCNTGSQKGKTAETFQPIETNVSARPAG
                     QKHVVGLTSPKMDTVRVGFIGLGMRGPGAVQRFTHIPGAKIVALCDLHPDRVEKSQQI
                     LDKAGVPRAAAYSGSEDAWKELCQRDDIDLVYIATDWKRHAQMMIYAMEQGKHVACEV
                     PAAMTMEEIWDVVNTAERTQKHCMQLENCVYDFFELTTLNMAQQGVLGDILYAEGAYI
                     HQLEDFWDSYENDWRMEYNKTHRGDIYATHGMGPACQVLDIHRGDKMNYLVAMDSKPV
                     SIPAYLKEKRGEEAPDFKNGQHTMTMIRTENGKTIHIQHDVASPRPYSRMYQVQGTNG
                     FANKYPNEGYALKADAVEAGTVPNHENLSAHSYVSDEVKKVLMDKYKHPIHIELEETA
                     KKVGGHGGMDFIMDYRLVYCLQNGLPLDMDVYDLAEWCSLAPLTALSLENNSAPVAVP
                     DFTRGHWNDVKGFRHAFVKN"
BASE COUNT          630 a          504 c          493 g          533 t
ORIGIN      
        1 gttatcttca tcggcgggat gaccggtttg tattgcctgg tagccacctc cgtttttatg
       61 tcattgatgt tcccaaccat ttacgggatc gcactcaaag ggttgggcga cgactcaacg
      121 ctgggagcgg ctggcctggt tatggccatt gtcggcggtg cattgatgcc tcccctgcag
      181 ggagccatta tcgatttggg caccatagct tggctacccg ccgtgaacgc atcgtttgtt
      241 ttgccattta tctgcttcac cgttatctgc atttacggtt tacggacaaa cagaaggaga
      301 attttgggct gaaagaggat atgttatata acatattaaa ggattattaa aaagtggtaa
      361 aaatacacta tttttgcact ttaaagcatt tgcttttttg cttttttaat gtatcaaccc
      421 caacttcggg tatattttct tataaacatc ccctggatta attaaaatat ttaaattatg
      481 aagaagctga tctatttatc catcatcaca accgttcttt ttgcatcctg taataccggt
      541 tcgcaaaaag gaaaaacggc ggagactttt caacccatcg aaaccaacgt ttcggctcgc
      601 cctgccggac aaaagcatgt ggtggggttg acctcaccca aaatggatac cgtccgtgta
      661 gggtttatcg ggctgggaat gcgtggcccg ggtgccgtac aacgattcac ccatataccg
      721 ggagcaaaaa tagtcgcact gtgcgacctt catcccgaca gggttgaaaa atctcaacaa
      781 attctcgaca aagcgggggt gccccgtgct gcagcataca gcggaagcga ggacgcctgg
      841 aaagaacttt gccaaaggga cgacatcgat ctggtttaca tagctacgga ctggaaacgt
      901 cacgctcaga tgatgatcta cgctatggag caagggaaac acgtagcttg tgaagtaccg
      961 gctgctatga ccatggagga aatctgggac gttgtcaaca cggcagaacg tacccagaaa
     1021 cattgcatgc aactcgaaaa ctgcgtgtat gatttctttg aactgaccac actcaacatg
     1081 gcgcaacaag gtgttcttgg agacatcctc tacgctgaag gcgcttatat ccatcaactg
     1141 gaagattttt gggattctta cgaaaacgac tggcgcatgg aatataacaa aacacaccgg
     1201 ggagacattt acgctacgca cggaatgggc cctgcctgtc aggttctcga catccaccga
     1261 ggagacaaaa tgaactacct ggtggctatg gacagcaaac cggtaagtat accggcttac
     1321 ctgaaagaaa aacgcggaga ggaagcgccc gattttaaaa acggacaaca caccatgact
     1381 atgattcgta ccgaaaacgg gaaaaccatc catattcaac acgatgttgc ctctcctcgt
     1441 ccatacagcc gtatgtatca ggtacagggg accaacggat ttgcgaataa atatccgaat
     1501 gaaggttatg cccttaaagc cgatgctgtt gaagccggca ctgtccccaa ccacgaaaac
     1561 ctgtccgctc actcttatgt gtcggatgaa gttaaaaaag tactgatgga taagtacaaa
     1621 catccgattc acatcgaact cgaagagact gccaaaaaag taggcggaca cggaggaatg
     1681 gactttatca tggattatcg cctggtctac tgcctgcaaa acggacttcc actggacatg
     1741 gacgtatacg acctggcaga atggtgcagc ctagctcctc ttacggcttt atcgctcgaa
     1801 aacaactctg ccccggtggc ggttcccgac ttcacgcggg ggcactggaa tgatgtaaaa
     1861 ggattcaggc acgcgtttgt aaagaactaa tccttttctc cccttaaccg ctctttgctg
     1921 ttagaaaaaa gacaagaagg ccacaattat tgatttcaac cccaccaatt tatacactaa
     1981 acatttgaac aatgtttggc ctaatggtta tgccgcttcc tgaaaaggac agaacggcat
     2041 aaccggcttt ttgccacaaa caatcttata accctaagga ttgtacaatt atgagtatta
     2101 atttaaaaat gagaacagaa tgaaaaaaag acattcaact actaaatgga aaggcctgtt
//
LOCUS       DAOSDR010000453         2152 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrimonas sp. isolate AMR_MDS_1826
            NODE_17694_length_2152_cov_4.513114, whole genome shotgun sequence.
ACCESSION   DAOSDR010000453 DAOSDR010000000
VERSION     DAOSDR010000453.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863355
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrimonas sp. (wastewater metagenome)
  ORGANISM  Petrimonas sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Dysgonomonadaceae; Petrimonas.
REFERENCE   1  (bases 1 to 2152)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2152)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:10:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,720
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 35
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2152
                     /organism="Petrimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17694_length_2152_cov_4.513114"
                     /isolate="AMR_MDS_1826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2023866"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..506)
                     /locus_tag="PLI30_13185"
     CDS             complement(<1..506)
                     /locus_tag="PLI30_13185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019538522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HOI80546.1"
                     /translation="MLDIFDRLKGMEGPLEQYRQKAEGYFMFPELEGEIGPRMKFHGR
                     EVITWSLNNYLGLANHPEVRKADAEAAATWGMAYPMGARMMSGQTKYHKELEERLAKF
                     EKKEAAYLLNYGYQGMVSIIDSLCTRHDVIVYDSESHACIMDGIFLHKAKGGKSFVFP
                     HNDMERAE"
     gene            818..2122
                     /locus_tag="PLI30_13190"
     CDS             818..2122
                     /locus_tag="PLI30_13190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009001065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylxylan esterase"
                     /protein_id="HOI80547.1"
                     /translation="MKKFSKQIVASSIFIIVFFSSLLAQPAQTLIDVNIAPEKSDWVY
                     KPNEKAKFNLSVTKNNIPVQNVSVWYEVGPEMMPPLKKENIVLKNGTATIDGGTLKEA
                     GFLRCRVVATYEGKEYAGLATAAFNPEGIRPTTDEPKDFIGFWDEAKAANAKIPMDAK
                     VRLLPERCTEKINVYEVNIQSYKPGTRLFGILCVPKAAGKYPAILRVPGAGVRPYNGD
                     TGNAEKGVITFEIGIHGIPVTMDASVYDNIRTASLDGYQNYNLDDKDRYYYKRVYLGC
                     VRAVDYIFSLPEFDGNNIVVLGGSQGGALSIVTAALDNRIKGLVAFYPALSDVTGYLH
                     NRAGGWPHFFNAANLSFNNSPQKIETSKYYDVVNFARHLKVPGFYSWGYNDVTCPPTS
                     MYAAYNVITAPKTLFLVEETGHWTYPEQWGMAYEFALNILGK"
BASE COUNT          612 a          432 c          468 g          640 t
ORIGIN      
        1 ttttcggcac gctccatatc gttgtgcggg aaaacgaaac tctttccgcc tttggctttg
       61 tgaaggaata tgccgtccat gatgcaggcg tgcgactcgg agtcgtatac aatcacatcg
      121 tgacgtgtac aaagcgaatc gatgatggag accattccct gatatccgta attgagaaga
      181 taagcagcct cctttttctc gaatttggcg aggcgttctt ccagttcttt gtggtatttt
      241 gtttgaccgg acatcattcg ggcacccata ggataagcca ttccccatgt tgctgcagct
      301 tccgcatccg ctttccttac ttcgggatga ttggctagtc cgagataatt gtttaaactc
      361 caggttatta cttcacgtcc gtgaaacttc attctgggtc caatctctcc ttcgagttca
      421 ggaaacataa aatagccttc tgctttttgg cgatactgct ccagcggacc ttccatacct
      481 tttagccggt caaaaatatc taacataaaa ttttaaatgt ttgattaaaa aaattagact
      541 ttgaaaatca agaaccaaga ctaatttatg ctataggatt agaatcctcg acttacgact
      601 tcttgatttc ttgtacaaaa ataatctttc tttctgaatt tgtgaaattt ccgtcttttt
      661 tgtttgtccc tgattatttt actgatcata atttattact tttgttttgt aaaaaaacaa
      721 aacagtttcc aggacgagct gaaaaggttc aaaacagtct tgaattcggt aaaaataact
      781 gtagaaaact ttcaggctcc atcaaaaaat aaataacatg aagaagtttt caaagcaaat
      841 tgttgcatcc tcaattttca ttatcgtttt cttttcaagt cttttggcgc aacctgcaca
      901 aaccctgatt gacgtgaata tagcgcctga aaagagtgac tgggtatata agcccaatga
      961 aaaggccaaa tttaacctgt ccgtcaccaa gaacaatatc cccgtgcaga atgtatctgt
     1021 ttggtacgaa gtcggccccg agatgatgcc tcctttaaag aaagaaaata tcgtgttgaa
     1081 aaacgggaca gcgaccattg atggcggcac cctgaaagag gctggatttt tacgttgtag
     1141 agtggtggcc acatacgagg gaaaagaata tgcaggattg gcaacggctg cttttaatcc
     1201 tgaagggatt cgtccgacaa cggatgaacc aaaggatttt atcggatttt gggatgaagc
     1261 aaaggctgcg aatgcaaaaa tacccatgga tgcaaaggtg aggttgttac ccgagcgttg
     1321 cacggaaaaa ataaatgtgt atgaggtaaa catacaaagc tataagccag gcacccgttt
     1381 gtttggtatt ttgtgcgtcc ccaaggcagc aggaaaatat ccggctattt tgcgtgtacc
     1441 tggagcggga gtgcgccctt acaatggaga tacaggtaac gcagaaaaag gtgttattac
     1501 ttttgagatc ggtattcatg gcattccggt aacaatggat gcatccgtgt acgataacat
     1561 cagaacggca tcgctcgatg gttatcagaa ttataacctg gatgacaaag atcgttacta
     1621 ttataaacgt gtttatttag gttgtgtacg tgcagtggat tatatcttca gtttacctga
     1681 attcgatgga aataatattg tcgtgctggg tggaagtcag ggcggtgctt tgtctatcgt
     1741 taccgctgct ttggataacc ggataaaagg gctggtcgct ttttaccctg ctctttccga
     1801 tgttacaggt tatttgcaca atcgtgcggg aggatggcct cattttttca atgcagctaa
     1861 cctttcgttc aacaattcac cgcagaaaat tgaaaccagc aaatattatg atgttgttaa
     1921 ttttgcgcgt catttgaaag tacccggatt ttattcatgg ggatacaatg atgtaacctg
     1981 tccgcctacc tccatgtatg ctgcttacaa cgtgatcacg gctccgaaaa cccttttcct
     2041 tgttgaagaa acagggcact ggacttatcc tgagcagtgg gggatggctt atgaatttgc
     2101 tcttaacata cttgggaaat aatcaaaccc atctgtaaaa taaaagagcc gc
//
LOCUS       DAOSDR010000454         2130 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrimonas sp. isolate AMR_MDS_1826
            NODE_17938_length_2130_cov_4.088675, whole genome shotgun sequence.
ACCESSION   DAOSDR010000454 DAOSDR010000000
VERSION     DAOSDR010000454.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863355
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrimonas sp. (wastewater metagenome)
  ORGANISM  Petrimonas sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Dysgonomonadaceae; Petrimonas.
REFERENCE   1  (bases 1 to 2130)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2130)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:10:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,720
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 35
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2130
                     /organism="Petrimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17938_length_2130_cov_4.088675"
                     /isolate="AMR_MDS_1826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2023866"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            150..710
                     /locus_tag="PLI30_13195"
     CDS             150..710
                     /locus_tag="PLI30_13195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019538298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4256 domain-containing protein"
                     /protein_id="HOI80548.1"
                     /translation="MEKNKITQEQKEKLFNILKKRFEKNIGRHPELSWKEIQEKLEGY
                     PEKLWSLNEMEETGGEPDVIGQDEKTGEFIFCDCSAESPKGRRSVCYDSEALRSRKEN
                     KPWNSAVGMAGEMGVALLNEEQYRNLQCLGKFDTKTSSWIATPDNIRQLGGAIFGDWR
                     YGQVFIYHNGAESYYAARGFRGLLKV"
     gene            complement(728..1213)
                     /locus_tag="PLI30_13200"
     CDS             complement(728..1213)
                     /locus_tag="PLI30_13200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019538204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HOI80549.1"
                     /translation="MEKGKRAIRVRREFNASLSLVWQAWTTPELLDRWWGPSPWRAET
                     QTMDFREGGYWLYAMVGPEGERHWSRADYISIVHEKSFTAKDGFCDENGTFDPEFPQN
                     LWETRFIETESTVMVEITLTFDTFADLEQTLAMGFREGFTTGLNQLDELLLVTPAEGR
                     Q"
     gene            1293..1730
                     /locus_tag="PLI30_13205"
     CDS             1293..1730
                     /locus_tag="PLI30_13205"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010259777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HOI80550.1"
                     /translation="MMTAEIHTAKGVMKVKFYEEDAPNTVANFVKLAEKGFYDGLTFH
                     RVIPNFVIQGGCPDGTGAGGPGYTIKCELNGNNQYHDRGVLSMAHAGRDTGGSQFFIC
                     HSRENTAHLDRNHTCFGKVYEGVEVIDKIRQGDRIEKILIFEE"
     gene            1895..>2130
                     /locus_tag="PLI30_13210"
     CDS             1895..>2130
                     /locus_tag="PLI30_13210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI80551.1"
                     /translation="MKRVRIIIAVLILFMLLGGILYINPLLPIITGYAAKNLSSGIFL
                     ANRTQESMEATDLNFSFIRFVKNRVDSRNKTVTS"
BASE COUNT          622 a          463 c          506 g          539 t
ORIGIN      
        1 aattaacagg atgaggcaaa ccaaaaagga gttgaagttt gttatttttt tcgcatcgca
       61 catgcaacac agatttagtt tccctgttca ggctaatcgg cctgagtgga aataactgat
      121 tggacatgag aaattattaa aaaaaaggta tggaaaagaa taaaatcacg caggagcaaa
      181 aagaaaagtt attcaatatt ctgaaaaaac gtttcgagaa gaatataggc cgccatccgg
      241 aacttagttg gaaagagata caagaaaaac tggaaggtta tccggaaaaa ctgtggtcac
      301 tgaatgagat ggaggaaacc ggaggtgaac cggatgttat aggccaagac gaaaaaacag
      361 gtgagttcat cttttgtgat tgctcggctg aaagtccgaa gggacgcaga agtgtttgct
      421 acgactccga agcattgcgg tcgagaaaag aaaataagcc ctggaacagc gccgtaggca
      481 tggctggaga gatgggagtc gcattgctga acgaggaaca gtaccggaat cttcaatgcc
      541 tggggaaatt tgacacaaag acttccagtt ggattgccac gcctgacaat atccgtcaac
      601 tcggcggagc catttttggt gattggcgtt acgggcaggt ctttatttac cacaatggag
      661 ccgaatccta ctatgctgcc aggggcttcc gtggattgtt gaaagtttag atttggagag
      721 attgatgcta ttgccttcct tccgcaggag taacaagcaa tagctcatcc agctgattca
      781 atcctgttgt aaagccttct ctgaagccca tggcaagggt ttgttcgaga tcagcaaagg
      841 tgtcaaaagt gagcgtaatc tccaccatca ccgtgctctc ggtttcaatg aaccgtgttt
      901 cccacaaatt ttgtggaaac tcaggatcga atgtcccgtt ttcatcgcaa aaaccatctt
      961 ttgcggtaaa ggacttttcg tgcacaattg aaatgtaatc cgccctgctc cagtgccttt
     1021 ccccttcggg gccgaccatc gcatagagcc agtatccacc ttcacggaaa tccatggttt
     1081 gggtctccgc tctccaaggg cttgggcccc accaccggtc gagcagttca ggggttgtcc
     1141 atgcctgcca gacgagtgag aggctggcat tgaattctct ccttacacgt atggcccttt
     1201 ttcccttctc cacggaaaaa acccctttta tttcacatcc agaataacta tttttgcaga
     1261 ttcataattt aataatctaa aaagtagaaa atatgatgac agcagaaata cacaccgcaa
     1321 aaggggttat gaaagtaaaa ttctatgagg aggatgctcc aaacaccgtg gccaacttcg
     1381 taaaactggc cgaaaagggt ttttacgacg gattgacgtt ccaccgggtg atcccaaact
     1441 ttgtgatcca gggcggatgt cccgatggca caggggcagg agggccgggc tacacaatca
     1501 aatgcgaact caacggtaat aaccagtacc acgatcgggg cgtattgtca atggcgcatg
     1561 ccggccgtga taccggaggg tctcagtttt tcatttgtca cagccgcgag aatacggctc
     1621 atctcgaccg taaccatact tgctttggaa aagtatacga aggggtcgag gtaattgaca
     1681 agatccgtca gggtgacaga attgagaaaa tcctgatttt tgaagagtaa tgatcaagca
     1741 agattgccta aaggtgattc atcgtactcg gatatgttcg ctcgtttaga cgtgaattaa
     1801 gaacacagcg atccctaaaa aaactatgat agtgtctaaa caaaagtagt ccttttttta
     1861 tttatctttg ctgatatatt caagtcaggc ttccatgaaa agagtacgca tcattatcgc
     1921 agtccttatt ctgttcatgc tcttgggggg tattctttac ataaaccctc tcctgccgat
     1981 tattaccggt tatgcagcta aaaacctgtc gtcgggcatc ttcctggcta accgcacaca
     2041 ggaatcgatg gaggcaaccg acctgaattt ctcatttatc cgattcgtga aaaacagggt
     2101 cgattccagg aataaaaccg tgacaagcca
//
LOCUS       DAOSDR010000455         2107 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrimonas sp. isolate AMR_MDS_1826
            NODE_18153_length_2107_cov_2.739279, whole genome shotgun sequence.
ACCESSION   DAOSDR010000455 DAOSDR010000000
VERSION     DAOSDR010000455.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863355
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrimonas sp. (wastewater metagenome)
  ORGANISM  Petrimonas sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Dysgonomonadaceae; Petrimonas.
REFERENCE   1  (bases 1 to 2107)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2107)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:10:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,720
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 35
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2107
                     /organism="Petrimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18153_length_2107_cov_2.739279"
                     /isolate="AMR_MDS_1826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2023866"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(237..1424)
                     /locus_tag="PLI30_13215"
     CDS             complement(237..1424)
                     /locus_tag="PLI30_13215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014224391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR domain-containing protein"
                     /protein_id="HOI80552.1"
                     /translation="MPNYTRHTDFLKDKQFIRWQLTPDEELDAHWEGFIKQNPELKIA
                     LQQAIDYLKTTGLNKSTLNEDERIRLLNEIQSTVERSLKRIKSRRLIQLSVASCAAIT
                     LLIIGLNHFVFQEKGTLFSPEQELIVGSLLNNEDIQLITDEKSLSFQNDVQMEFDESG
                     KAKITQGNNGGKTAELTGSKQNTLIVPYGKRSTLTLSDGSKVWLNSGSVLEFPAQFTE
                     NKREIRLASGEMYIEVAPDSQKPFHVSTSDFNVKVYGTKFNISAYADSPRSVVLVEGK
                     VSLKPTNKKETFLSPSEQAIYSDNGTFNRQKVDVNQFISWKNGYLEFDKTPMTEVLKQ
                     IGRYYNLSFDFDNDVNLQKRTCTGKIYLSENLDNVMTTIGLLSSTKYIKDNNQVYIIN
                     ESN"
     gene            complement(1538..2071)
                     /locus_tag="PLI30_13220"
     CDS             complement(1538..2071)
                     /locus_tag="PLI30_13220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014224390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOI80553.1"
                     /translation="MRKENIASLYNRYVDDLYTYALYLGFEKGIIMDAIHDVFCKFAA
                     DEKQLQDVSNLKFYLFKSLKNRIYDIYKAQKKYIGLSTIDAQDAPFNIQVTIEDRLID
                     KEEQQQIKDQLSEMLDSLTERQREVVYLRYVQEYDYAQISELLNISIHGCRKLLSKAM
                     QNLREKYGALVFLFLFS"
BASE COUNT          554 a          390 c          431 g          732 t
ORIGIN      
        1 aggaaaacaa tatgcagaaa aacaagaaga ataaggctat tctcatgatt tttagataat
       61 gtttaaaatt attgagaggt tctctcccct caattgaatg tttattcata tctttgtacg
      121 attttaatta ttaataatcc aggtaattaa attcaatctg gccggacggt attcgcagta
      181 tcgttcggct tttggttttt gttattatca tctgtctttg tgcataggct taaaatttaa
      241 ttggattcgt taattatata tacttgattg ttgtctttta tgtatttagt tgaggataaa
      301 agccctattg tggtcatgac gttatccaga ttttctgaga gataaatttt tccggtacag
      361 gtccgcttct gtaggttcac atcgttatcg aagtcaaaag agaggttgta gtatcttccg
      421 atctgtttca gtacttctgt cataggggtt ttatcgaatt ccaggtaacc atttttccaa
      481 ctgataaact gattcacatc cactttttgc ctgttgaatg taccgttgtc cgaataaata
      541 gcttgttcgc tgggcgataa gaaagtttcc tttttgtttg tgggtttcag actcactttg
      601 ccttccacca acactaccga tcgtggggaa tcggcataag cggagatatt gaacttggtg
      661 ccgtacacct ttacgttaaa atcggaagtg cttacgtgaa acggtttttg gctgtcgggt
      721 gctacttcaa tatacatttc tcccgacgcc agccggatct cccgtttgtt ttcggtaaat
      781 tgggccggaa attcaagtac ggaaccggaa ttgagccaca ctttactccc gtcggaaagg
      841 gtaagtgtgg agcgttttcc gtatgggacg attagtgtat tttgtttgct tccagttaat
      901 tctgcagttt ttcctccatt gttcccctgt gtaatttttg cttttccgct ttcatcgaac
      961 tccatttgca catcgttttg gaacgatagc gatttctcgt ccgtaatcag ttgaatgtct
     1021 tcgttgttta acagacttcc aacaatgagt tcttgttccg gtgaaaacag ggttcccttt
     1081 tcttggaaaa caaagtggtt taatccaata ataagtaagg taatagctgc gcaggaagca
     1141 acggagagtt gaataagccg gcggcttttt attcttttca acgatcgttc gacggtggat
     1201 tgaatttcat tcaacaaacg tatacgttca tcctcgttta aggtgctttt gttcagtccg
     1261 gttgttttaa ggtagtcaat ggcttgctgt aaggcaattt ttaactccgg gttttgtttg
     1321 ataaagcctt cccaatgcgc atctagctct tcatctggcg ttaattgcca gcggataaac
     1381 tgcttgtctt ttagaaaatc ggtatgtcgt gtataatttg gcatcgtaaa ttcatttcat
     1441 gtgactacca gttagaagca cgttcattaa atagacgact acgggaaaaa tttatcccct
     1501 tgtttttgaa aaatcctata aatttaacac tttcgcgtta agaaaagaga aaaaggaaaa
     1561 cgagtgcacc gtacttttca cgtaggtttt gcatggcttt tgagagaagt ttccggcatc
     1621 cgtggatact gatgttcagc aattcggaga tttgtgcata atcgtattcc tgaacataac
     1681 gtaggtaaac cacttcacgt tgacgttcgg taaggctgtc aagcatttcc gagagctggt
     1741 cttttatctg ttgctgctct tctttgtcga taagccggtc ctcgatggta acctggatat
     1801 tgaaaggcgc atcctgcgcg tcaatggttg ataatccaat atattttttt tgcgctttat
     1861 aaatatcgta tattctgttt ttcagtgatt tgaaaagata aaatttcagg tttgaaacat
     1921 cttgaagctg cttttcatcg gccgcaaatt tacaaaatac atcgtgaata gcatccataa
     1981 tgatcccttt ctcaaaacca agatagaggg catacgtgta caaatcgtcc acgtaacggt
     2041 tatagaggga tgcgatgttt tcttttctca tttattttgc aaatttttac aaaatgaaat
     2101 acaaaat
//
LOCUS       DAOSDR010000456         2092 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrimonas sp. isolate AMR_MDS_1826
            NODE_18303_length_2092_cov_3.551792, whole genome shotgun sequence.
ACCESSION   DAOSDR010000456 DAOSDR010000000
VERSION     DAOSDR010000456.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863355
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrimonas sp. (wastewater metagenome)
  ORGANISM  Petrimonas sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Dysgonomonadaceae; Petrimonas.
REFERENCE   1  (bases 1 to 2092)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2092)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:10:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,720
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 35
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2092
                     /organism="Petrimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18303_length_2092_cov_3.551792"
                     /isolate="AMR_MDS_1826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2023866"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..459)
                     /locus_tag="PLI30_13225"
     CDS             complement(<1..459)
                     /locus_tag="PLI30_13225"
                     /inference="COORDINATES: protein motif:HMM:NF014997.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HOI80554.1"
                     /translation="MSNEKFYTAREEKANYLTHAAGVLMALVATVLLLRKAILADNRW
                     AMLAYAIFGFGMLACMLSSTVYHFVQEPGRKVKWRHFDHASIYLLIAASYSPFTLILL
                     REERFWGWLLFGLVWIIALVGIAVSFGELKKNSHLKTASYVFMGLVVLVAF"
     gene            complement(452..1636)
                     /locus_tag="PLI30_13230"
     CDS             complement(452..1636)
                     /locus_tag="PLI30_13230"
                     /inference="COORDINATES: protein motif:HMM:NF025092.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HOI80555.1"
                     /translation="MNLKDYIHGKRYGKEANRLEREAMNDPFLQDTIDGYDSVPGNHV
                     EAIEKLEKRFAPQPKHIDKRGWLWAAAAVLVLLIGLPFLLRQPDTKDVQVASVESLKK
                     EETTLSPKRDSVLVADHTPPKTAEERTATTVQKKVEVRVKEVQQAVEAEKTAEIVSEE
                     ILQLQAPERMAEAIEPEQVSVARAALPKQKAIQGKVAAVTAVSPNTKTISGKIVDEIG
                     EPIIGATVNVKNTHLGAVTDMDGKFRLTVPEDEKGMLVASFIGMKNVEMPLKEHLGDV
                     VMKSNDMALDEVVVVAFGTQKNESIVGSASTVKDPAPVFGEAEFRHYFEKNHDKTVCP
                     DEPISIKVEFYVNGQGRPGSIAIKENSCQKLETEIKRLLLGSPPWSERNRKVTLNWTL
                     NE"
     gene            complement(1656..>2092)
                     /locus_tag="PLI30_13235"
     CDS             complement(1656..>2092)
                     /locus_tag="PLI30_13235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019540780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOI80556.1"
                     /translation="VMDLFEDLNQKITNYEISNFHSWLYTVAKNHCLLKIRKDKHVFF
                     EDINESVMENGDFFTPLDEEKTNEEKSALDYCMAKLGSEQRTSIEYFYYESKSYADIV
                     SLTGYTLEKVKSYIQNGKRNLKNCILRLRALNEEQRVQTRNT"
BASE COUNT          508 a          508 c          436 g          640 t
ORIGIN      
        1 aaaagcaacc aaaaccacta aacccataaa aacatacgat gcggtcttta aatgactgtt
       61 tttctttagt tcaccaaaac tgacggcaat accaacaagc gcgatgatcc agaccagtcc
      121 gaacagtagc catccccaga agcgctcctc gcgcaataaa atcagcgtga acggtgagta
      181 gcttgccgcg atcaggaggt agatgcttgc atggtcgaag tggcgccatt tcacttttcg
      241 tccaggctcc tgcacaaaat gataaacggt ggatgaaagc atgcaggcca gcatcccgaa
      301 accgaaaatg gcgtaagcta gcattgccca acggttatcg gcaagtatcg ccttgcgaag
      361 caggaggaca gtcgcaacca gggccattaa aaccccggca gcgtgggtga ggtaattggc
      421 cttttcttcc cgcgcggtgt aaaatttttc gttactcatt caatgtccaa tttaacgtta
      481 ctttccggtt acgttccgac cagggaggag atcccaacaa caaccgcttg atctcggtct
      541 ccaatttctg acacgagttt tctttaatag cgatgctgcc cggtcgtcct tgcccgttca
      601 cataaaattc cactttaatc gaaatcggct catccggaca aacggtttta tcgtgatttt
      661 tttcgaaata atgcctgaat tcagcttctc cgaaaacagg agcagggtct tttaccgttg
      721 atgcggaacc tacgattgat tcatttttct gagttccaaa agcaacaaca accacttcat
      781 ccaaagccat atcgtttgat ttcatcacaa cgtcacctaa atgttctttt aaaggcattt
      841 caacattttt cattcctatg aaagaagcaa ccagcatgcc tttctcatct tcgggaacgg
      901 ttaaccggaa tttcccgtcc atatcggtga ctgctcctag atgggtattt ttgacgttta
      961 cggttgctcc gataatgggt tctccaatct catccacaat ttttcccgaa atggtttttg
     1021 tattcgggga gacggcagtt acggcagcca ccttaccctg aatggctttc tgtttgggta
     1081 aggcggctct tgcaactgat acctgttccg gttcaatagc ctctgccatc ctttcgggtg
     1141 cttgaagttg caaaatttct tcgctgacaa tttcggcagt tttttcagcc tcaacagctt
     1201 gctgtacttc ttttacccga acttcaactt ttttttgcac ggtagttgcc gttctttctt
     1261 ctgctgtttt tggcggcgtg tgatcggcca cgagaacgga atctcttttg ggagaaagag
     1321 tggtttcttc ttttttcagg gattctaccg atgcaacctg aacgtctttt gtatcgggtt
     1381 ggcgaagcag aaagggtagg ccgataagca aaacaagcac ggcagccgca gcccatagcc
     1441 agccgcgttt atcgatgtgt ttgggttgcg gagcaaaccg tttttccagc ttttctatag
     1501 cttcaacatg gttgcccggc accgaatcgt aaccgtcgat ggtgtcttgc aggaacggat
     1561 cgttcatggc ttcccgttct aagcggttag cctctttccc gtaccgtttt ccgtgtatgt
     1621 aatcttttaa attcactttg tcaaaatgtc gcgtgttacg tgttgcgtgt ttggaccctt
     1681 tgctcttcgt ttaatgctct tagccgtaaa atgcaattct tcaaattcct ttttccgttc
     1741 tggatgtaac tttttacttt ctctaacgta tagcctgtca gggaaacgat gtcggcatac
     1801 gacttgcttt cgtaataaaa atactcgatg ctcgtccgct gctctgaacc taactttgcc
     1861 atacaataat ccagcgccga tttttcttcg ttcgtcttct cttcatctaa tggagtaaaa
     1921 aaatcgccgt tttccataac cgattcgttt atatcttcga aaaaaacgtg cttgtctttc
     1981 cttatcttca ataaacagtg atttttagcc acggtataaa gccatgaatg aaagttgctt
     2041 atctcgtagt ttgtgatctt ttgattcaga tcttcgaata aatccattac gg
//
LOCUS       DAOSDR010000457         2088 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrimonas sp. isolate AMR_MDS_1826
            NODE_18350_length_2088_cov_3.784063, whole genome shotgun sequence.
ACCESSION   DAOSDR010000457 DAOSDR010000000
VERSION     DAOSDR010000457.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863355
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrimonas sp. (wastewater metagenome)
  ORGANISM  Petrimonas sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Dysgonomonadaceae; Petrimonas.
REFERENCE   1  (bases 1 to 2088)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2088)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:10:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,720
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 35
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2088
                     /organism="Petrimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18350_length_2088_cov_3.784063"
                     /isolate="AMR_MDS_1826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2023866"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..704
                     /locus_tag="PLI30_13240"
     CDS             <1..704
                     /locus_tag="PLI30_13240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOI80557.1"
                     /translation="PCVMPDGLTIYFSSTGHGSLGGYDLFVTRYNLATNSYLTPNQLN
                     MPFNSPFNDYMMAIDEEKGIGWFASDRFQPKDSVCVYTFVPSPEVVLLENEDASYLAN
                     RARISSIKESWKPDIDYSALLQRSHKTTAPQKTTQKKDFTFVINDEKTYYTLTDFKNA
                     TARSLFSQAINLKTRLNQQEKDLSEKRRQIAGSSNSSALSASILNLEKETENLFREIE
                     SLELRARNEEIRNSF"
     gene            716..1207
                     /locus_tag="PLI30_13245"
     CDS             716..1207
                     /locus_tag="PLI30_13245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009275728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HOI80558.1"
                     /translation="MTFNVIIVAVLIVLGILLLLIEFFLLPGISIAGVGGAIFMVGGV
                     IYSYIYLGSTAGNITLALSLILLALAFVWLLKSKSLQKIALTADIRETVDNSDLKSLQ
                     PGDTGITVSRLNPIGKVMINEVTVEGKSFDGELIDEDTEIEVVRVESYNVTVKKREQE
                     AKI"
     gene            1285..>2088
                     /gene="floA"
                     /locus_tag="PLI30_13250"
     CDS             1285..>2088
                     /gene="floA"
                     /locus_tag="PLI30_13250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005785715.1"
                     /note="flotillin-like protein involved in membrane lipid
                     rafts; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin-like protein FloA"
                     /protein_id="HOI80559.1"
                     /translation="MELSFPLIIGIALIVIFVLIFFHYVPFFLWINALSANVRISLVQ
                     LFLMRLRRVPPHTIVYAMIEAHKAGLKNVTRDNLEAHYLAGGHVEQVVHALVSASKAN
                     IELPFQMATAIDLAGRDVLEAVRMSVNPKVIDTPPVSAVAIDGIQLIAKARVTVRANI
                     KQLVGGAGEETILARVGEGIVSSIGSAASHKSVLENPDSISKLVLRKGLDAGTAFEIL
                     SIDIADIDIGKNIGAVLQMDQAQADKNIAQARAEERRAMAIALEQEMKAK"
BASE COUNT          588 a          483 c          471 g          546 t
ORIGIN      
        1 acccgtgtgt tatgcccgat ggccttacca tttatttctc ctccaccggg cacggttcat
       61 tgggtgggta tgatctgttt gttacccgat ataacctggc cacaaactcg tacctaaccc
      121 ctaatcagct gaacatgccg tttaactccc ccttcaacga ctacatgatg gctatcgatg
      181 aagaaaaagg tatcggctgg ttcgccagcg accggtttca accgaaagac tccgtttgtg
      241 tttatacgtt tgttccctct cctgaagttg tgctgctgga gaatgaagat gcgtcttacc
      301 tggcaaaccg ggcaaggata tcctccataa aggaatcctg gaaaccagat attgactatt
      361 cagccttgct gcaacgttca cacaaaacaa ctgctccaca aaagactacg caaaaaaaag
      421 attttacgtt tgttattaac gacgaaaaaa cctactatac gttgacagat tttaaaaatg
      481 caacggccag gtcgctcttt tctcaagcca tcaacttgaa gaccagactg aatcaacagg
      541 aaaaggacct atcggaaaaa cgccggcaaa ttgcaggaag ttccaactca tctgcactat
      601 cggcgtccat attaaatctt gaaaaggaaa ccgaaaatct attcagagag attgaatcgc
      661 tggaattacg ggcgcgcaat gaagagatca gaaacagttt ttaattcttt acatcatgac
      721 tttcaatgta atcattgttg ccgttttaat cgttctggga attttactac ttctcattga
      781 gttttttctg ttaccgggta tcagtattgc cggagtaggc ggcgctattt ttatggtagg
      841 cggcgtgatc tactcctaca tctacctagg ttctacagcc ggcaacatta cactggccct
      901 ttcgttaatt ttgcttgcac tggcatttgt gtggctgcta aaatccaaat cactgcaaaa
      961 gatagcgctt actgccgaca ttcgtgaaac ggtggacaac agcgacctga aaagccttca
     1021 gcctggtgat accggaataa cggtttcccg gttgaatcct atcggaaagg tgatgataaa
     1081 cgaagtaact gttgaaggga aatccttcga tggggaactt attgatgaag atacagaaat
     1141 agaagttgta cgggtagaat cgtataatgt gacggttaaa aagagagaac aagaagcaaa
     1201 gatataagac agaaggctct cagatactca aacaagaacg tcgaacttta aatattgaac
     1261 tttaaacacc aaataaatta aattatggaa ttatcttttc ctctgattat tgggattgca
     1321 ttaattgtaa ttttcgtact gatttttttc cactatgtgc ctttcttttt gtggattaac
     1381 gctctctcgg caaacgtccg aatatcgctt gtccagttgt tcttgatgcg tttacgccgg
     1441 gttccgcctc acaccatcgt ctacgcgatg attgaagcgc acaaggccgg attgaaaaac
     1501 gttacccggg acaacctgga agcgcattac ctggccggag ggcacgtgga acaagttgtc
     1561 catgcgcttg tttcggcatc aaaagccaac atcgaactgc cttttcagat ggccacagcc
     1621 attgacctcg caggccgtga cgtactggaa gccgtaagga tgtcggtaaa ccccaaggtt
     1681 attgacacac cgccggtttc ggcagtagcc atcgatggta ttcaactaat tgccaaagcg
     1741 cgcgttacgg tccgggccaa catcaagcaa ttggtgggtg gtgccggtga agagaccatc
     1801 cttgcccgcg ttggtgaagg aattgtatcc tctatcggtt cggcagcttc gcacaaatcg
     1861 gtgctcgaaa accccgactc catttcgaaa cttgtgttaa gaaaaggatt ggatgccggg
     1921 actgcattcg aaattctctc tattgatatt gctgatatcg atataggtaa aaatatcggt
     1981 gccgtcctgc aaatggatca ggcacaagcc gacaagaaca tcgcccaggc ccgcgccgaa
     2041 gagcgccgcg ccatggctat cgcccttgaa caggagatga aagccaag
//
LOCUS       DAOSDR010000458         2086 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrimonas sp. isolate AMR_MDS_1826
            NODE_18374_length_2086_cov_3.912851, whole genome shotgun sequence.
ACCESSION   DAOSDR010000458 DAOSDR010000000
VERSION     DAOSDR010000458.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863355
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrimonas sp. (wastewater metagenome)
  ORGANISM  Petrimonas sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Dysgonomonadaceae; Petrimonas.
REFERENCE   1  (bases 1 to 2086)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2086)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:10:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,720
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 35
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2086
                     /organism="Petrimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18374_length_2086_cov_3.912851"
                     /isolate="AMR_MDS_1826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2023866"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..564)
                     /locus_tag="PLI30_13255"
     CDS             complement(<1..564)
                     /locus_tag="PLI30_13255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013816662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HOI80560.1"
                     /translation="MITLQLINPKSIAVIGASENIEKPGGKALYNVKNGTFEGGLYAV
                     NPKHENIQGVQTFRNVSELPQIDLAILAIPANLCVPVIKSLLEEKNTKAVVVLSAGFS
                     EMGPIGKKMEKEMALLADKHQATLIGPNCIGVMNPYHQSVFVSPVPKMEWGGTALVSS
                     SGATCVFMLESALSKGLTFSSVFSVGNA"
     gene            748..>2086
                     /locus_tag="PLI30_13260"
     CDS             748..>2086
                     /locus_tag="PLI30_13260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019539261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HOI80561.1"
                     /translation="MLSTLFLLAACTGNKSETSQVSAEPDLPDSTFQYKVDRFADVEI
                     LRYYVPGFKTLPLAQKELIYYLSQAALEGRDILFDQHNRYNLTIRRVCEGVYENYMGD
                     KSSEDWKNFETYLKQIWMANGIHHHYSEDKILPKFSQDYFVSIVKGVDPGRMPFRDGM
                     AADLTLKEILPVMFDANVMPKRMNQSAGADIVATSAVNFYEGVTQKEVEDFYNTMKDP
                     NNLTPVSYGLNSKVVKKDGVVSEQVWKLGGMYDKAIERIIGWLEKAAGVAENEQQKEV
                     INTLISYYRSGDLKTFDDYSVKWVTDTASRVDFVNGFIETYTDPLGMKATWESMVNFK
                     SEEASKRTQLISDNAQWFETHSPIDDKFKKEEVKGVSAKVITAAILGGDCYPATPIGI
                     NLPNANWIRRDHGSKSVTIDNITSAYDNAAQGSGFNEEFMWSDAERELSKKYGS"
BASE COUNT          615 a          472 c          477 g          522 t
ORIGIN      
        1 tgcatttcct accgagaata cggaagaaaa agtaagccct ttgctcaatg ccgattccag
       61 cataaataca caagtagctc ctgagctgga aacaagtgct gtgcctcccc actccatttt
      121 cggtacggga gagacgaaaa ccgactgatg atatggattc ataaccccga tgcagttggg
      181 gccaatcagc gttgcctgat gcttgtccgc caggagcgcc atctctttct ccattttttt
      241 acctatcggt cccatttcgg aaaatccggc agagagcaca acgacagctt tggtattttt
      301 ctcttcaaga aggcttttta taaccgggac acacaggttg gccggaatag ccaggatggc
      361 cagatcgatc tgtggcagtt ccgatacatt cctgaaggtt tgcacaccct gaatgttttc
      421 gtgctttggg tttacagcat agaggcctcc ttcaaacgtt ccgtttttca cgttataaag
      481 tgcttttccg ccgggttttt cgatgttctc cgaagcaccg atcacggcaa tgcttttcgg
      541 gtttatcagt tggagcgtta tcatatataa ttcagaattc aaccgttaga ggccagaaaa
      601 cgacagataa agtatcattt cgaaagtcca aagttacaat tttccaacaa aaaacgtaat
      661 ttttacgtat aatttgtgaa gttaaattta ttatctttgc atccaaacta aacaaatcta
      721 taataaaaaa tgaaaaaagt tttttttatg ctatcaaccc tttttctgtt ggctgcctgc
      781 acaggcaaca aatcagaaac ctcgcaggta tctgctgaac cggatctgcc ggattccact
      841 ttccagtata aagttgaccg gttcgccgat gtggaaatac tccgttatta tgtcccggga
      901 tttaagactt tgcctttagc gcaaaaagaa ttaatctatt acctgtctca agcagctctt
      961 gaaggacgcg atatcctatt cgaccagcat aaccggtaca acctgactat tcgtagggta
     1021 tgcgaaggcg tttatgaaaa ttacatgggc gacaaatctt cggaggactg gaagaacttc
     1081 gaaacttacc tgaaacagat ttggatggca aacggcatcc atcaccacta ttccgaagat
     1141 aaaatcctgc cgaaattttc gcaggactat ttcgtgtcga tcgtcaaagg ggtggacccc
     1201 ggccgtatgc ctttccgcga cggaatggca gccgatttga cattgaagga aatccttccg
     1261 gtgatgttcg acgccaacgt gatgcctaag agaatgaacc agtctgccgg agccgatatt
     1321 gtagcaacat cggcagtgaa cttctacgaa ggggtgaccc aaaaagaggt agaagatttc
     1381 tacaacacaa tgaaagatcc gaacaacctc acgccggttt cctacggatt aaacagcaag
     1441 gttgtgaaaa aagacggtgt tgtatccgaa caagtgtgga agttgggtgg aatgtatgac
     1501 aaagccattg agcgcattat cggatggctg gaaaaagcag ccggtgtcgc tgaaaatgag
     1561 cagcagaaag aagtcatcaa cacgttgatt tcctattaca ggtcgggtga tctgaaaaca
     1621 ttcgacgatt actcggttaa atgggtaacc gataccgctt cgcgcgtaga ttttgtcaac
     1681 ggttttatag agacctatac cgatccgctg ggaatgaaag caacctggga atccatggtg
     1741 aacttcaaaa gcgaagaagc gtccaaacgt acgcaactta tcagcgataa cgcacagtgg
     1801 tttgagaccc actcacccat tgacgacaaa tttaaaaaag aagaggtaaa aggcgtatcg
     1861 gcaaaagtaa tcacggcagc catcctggga ggagactgct atccggcaac ccccattgga
     1921 ataaatcttc ccaacgccaa ctggattcgc cgcgaccacg gatcaaaatc agtgactatc
     1981 gacaacatta catcggctta cgacaatgcg gctcagggaa gcgggttcaa cgaggagttc
     2041 atgtggagtg atgccgaacg tgaactgtcg aaaaaatacg gttcgt
//
LOCUS       DAOSDR010000459         2059 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrimonas sp. isolate AMR_MDS_1826
            NODE_18672_length_2059_cov_2.731038, whole genome shotgun sequence.
ACCESSION   DAOSDR010000459 DAOSDR010000000
VERSION     DAOSDR010000459.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863355
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrimonas sp. (wastewater metagenome)
  ORGANISM  Petrimonas sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Dysgonomonadaceae; Petrimonas.
REFERENCE   1  (bases 1 to 2059)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2059)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:10:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,720
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 35
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2059
                     /organism="Petrimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18672_length_2059_cov_2.731038"
                     /isolate="AMR_MDS_1826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2023866"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1030)
                     /locus_tag="PLI30_13265"
     CDS             complement(<1..1030)
                     /locus_tag="PLI30_13265"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOI80562.1"
                     /translation="MKLQRRDFIKGLVAVPVLGFFAAGVKGNISKQVEKKTIDYYKRL
                     KIDRLEAPLEKLTPPTGTKGKRIRIGLVGCGWRGQALLQKFGYLHSDVIEQHTKNGEP
                     SNWLRNLMAQEDLNVEVTGICDVFDVHTQRGVDISRHNVYPSNTREPKTAEVFQSYRD
                     MVKSENIDAILIATPDHTHVPMAIAAANAGKHVYLEKPMAQSIEAAIALRDTIKKCNV
                     VFQVGHQNRQQMSYKVGRELFQKGLLGDVTQVETFTNRNTVDGAWIRDDVRDHKLGNE
                     SNINWKEFLHDAPWVEFDRKRFFSWQRYSEYGTSVTGNDFAHWFDCVNQVLDLGIPES
                     VMAMGGQYY"
     gene            complement(1129..>2059)
                     /locus_tag="PLI30_13270"
     CDS             complement(1129..>2059)
                     /locus_tag="PLI30_13270"
                     /inference="COORDINATES: protein motif:HMM:NF019592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HOI80563.1"
                     /translation="MQDWSNSPEVMFWREYNNDLSRGESAFTNDRNHRMEFPNNNSIT
                     KQLADSYLCNDGLPISVSPLFEGHETLIDEMKNRDPRFFQNIAPHDGIQKINKDGTKV
                     YWSAVYNKLNTDAYHNSPAGYINIKGYNPDMNFHVQQYEESPGLIYRYAEALLNYAEA
                     KAESGSITQADLDLSINKLRKRVGMPDLKLDNIVTDPNWDFPTLSPIINEIRRERRVE
                     LACEGYRWDDIARWAAADELIVGKRPKGFKASQVAKNPYPVDAEGFLDPFQKALPSGY
                     GFILGRDYLNSIPISELVLNPNLIQNPGWEGID"
BASE COUNT          526 a          506 c          388 g          639 t
ORIGIN      
        1 agtagtactg accacccata gccattaccg attcgggtat accaaggtcg agcacctggt
       61 tgacacaatc aaaccagtgt gcaaaatcgt tacctgtaac acttgtaccg tactcactgt
      121 aacgctgcca gctaaaaaaa cgtttacgat caaattccac ccagggtgca tcatgcagga
      181 actctttcca attgatgtta ctctcattac ccagtttatg atctctcaca tcgtccctta
      241 tccaggcacc atccaccgta tttctattcg tgaatgtttc tacttgcgtc acatcgccca
      301 acaaaccctt ttggaataac tcccggccta ccttgtaact catctgttgc ctgttctgat
      361 gccctacctg aaaaacaaca ttacacttct taatggtgtc ccttaatgca atggcagcct
      421 cgatggattg ggccatcggt ttttccagat aaacatgttt accggcattg gcagcggcaa
      481 ttgccatagg tacatgggta tgatctggcg ttgctatcag gatagcgtca atattctcgc
      541 tttttaccat gtcgcggtag gattgaaata cttctgctgt ttttggttca cgcgtattgc
      601 taggataaac gttgtggcga gagatatcta ccccacgttg tgtgtgcacg tcaaaaacgt
      661 cacaaatacc tgttacctct acattcagat cttcttgcgc catcaggtta cgcaaccagt
      721 ttgatggttc accatttttg gtgtgttgct cgatcacgtc agaatgtaaa taaccaaatt
      781 tctgcagcag ggcttgtcct ctccagccac agcctaccag tccgattctg attcttttgc
      841 cctttgtgcc cgtgggaggc gttagcttct caagaggagc ttccaacctg tcaatcttta
      901 gtcgcttgta gtaatcaatt gtctttttct caacctgttt cgaaatgttc cctttcactc
      961 cggcagcgaa gaaacctagc acgggtacgg caaccaaacc ttttataaaa tcccttcttt
     1021 gtaatttcat gggtctattc aactaatcaa atgaaaattt aaaatatctt ttccttaaaa
     1081 aaatgataaa gttgggggaa gtattttccc caactttatc ggttccgctt aatcaattcc
     1141 ttcccatcct gggttctgaa tcagattagg atttaaaacc agctctgaaa tgggaattga
     1201 attgagataa tcccttccca agataaatcc gtatccgctg ggcaatgctt tctgaaaagg
     1261 atccaaaaac ccttctgcgt ctaccggata aggatttttc gctacctgcg atgctttaaa
     1321 tcctttgggt cgttttccta cgataagctc gtcagcagct gcccaacgtg caatatcatc
     1381 ccacctgtat ccttcacagg caagttctac tctcctttcc cttcttattt cgttgataat
     1441 tggcgacaaa gtagggaagt cccagtttgg atctgtaaca atattatcca gtttcaaatc
     1501 cggcataccc actctctttc tgagtttatt gatggataaa tccaaatcag cctgtgtaat
     1561 tgaaccagat tccgccttcg cttctgcgta atttaacaac gcttccgcat acctgtagat
     1621 cagaccggga gattcttcat actgctgtac atgaaaattc atatcggggt tgtaaccctt
     1681 gatatttata tagccggcag gagaattgtg ataagcatca gtatttaatt tattgtaaac
     1741 tgcactccaa taaactttcg tcccgtcttt attaatcttt tggatcccgt catgcggagc
     1801 tatattctgg aagaagcggg gatccctgtt tttcatctcg tcaatcagcg tttcatgtcc
     1861 ttcaaaaaga gggctcacac taataggcaa cccgtcgtta cacaagtaag agtctgccaa
     1921 ctgtttagta atggagttat tgttgggaaa ctccattctg tgattcctat cgttagtgaa
     1981 agccgattct cccctggaaa gatcgttgtt gtattcccgc cagaacatta cctccgggct
     2041 atttgaccag tcctgcatt
//
LOCUS       DAOSDR010000460         2050 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrimonas sp. isolate AMR_MDS_1826
            NODE_18775_length_2050_cov_3.562907, whole genome shotgun sequence.
ACCESSION   DAOSDR010000460 DAOSDR010000000
VERSION     DAOSDR010000460.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863355
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrimonas sp. (wastewater metagenome)
  ORGANISM  Petrimonas sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Dysgonomonadaceae; Petrimonas.
REFERENCE   1  (bases 1 to 2050)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2050)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:10:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,720
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 35
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2050
                     /organism="Petrimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18775_length_2050_cov_3.562907"
                     /isolate="AMR_MDS_1826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2023866"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1080
                     /locus_tag="PLI30_13275"
     CDS             <1..1080
                     /locus_tag="PLI30_13275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005808076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HOI80564.1"
                     /translation="PYIEISVNRQEMARYGLTVADLQEVISAAVGGMSLTTTVEDRER
                     FPVRLRYPRELRENPDELARLIVPTATGAQIPLGDVTDITYKRGAQMIQSENTFLIGY
                     VIFDKVEGKAEVDVVQEAQRVLDEKIRTGEMQLPKGVSYTFAGNYEQQARAAQRLAII
                     IPISLLVILLILYFQFKTITASLIHFSGIFVALAGGFILLWLYGEPWFMNFDIGGMNM
                     RDMFNMQPINLSIAVWVGFIALFGIATDDGVLMGTYIHDAFLEKDPQTREEVRETVVY
                     AGKKRVRPAVMTTATTLIALLPVLTSTGKGSEIMVPMSIPTFGGMVIQMMTMFIVPIL
                     QCWWREGVIRKKEKQQAKIADYETI"
     gene            1067..>2050
                     /locus_tag="PLI30_13280"
     CDS             1067..>2050
                     /locus_tag="PLI30_13280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019541071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HOI80565.1"
                     /translation="MKQYNVNLLFIILLGFIITLPVKGQTADSLARYLEIAGRNNPGL
                     NADFLAYKASLEKVPQAGAWPDPKLDIGFFLKPMDIVGGRQIADFTLMQMFPWFGTKK
                     AAQTEATHMAKMSYEQFRETRDNLYLEVYTQWYVLSTLVQQLNNNRENLQLLKQLEEL
                     AIRKVSSPYSGSSSGYSVPAPSPATKSSNSSPTGGGMSGMGSMGGSTTSSGTSSSSMS
                     GMSSSGGSMSSGMGSTAPGMSDVLRVQLEMVEIENNIESLLSDIAAEKARFNALLNRS
                     VDFEVVVPDSIVKVDYLFDKASSMREIENQNPMLGMLKEEELAYRAKREMDL"
BASE COUNT          560 a          459 c          511 g          520 t
ORIGIN      
        1 ccctatattg agatcagcgt aaaccggcaa gagatggctc gatacggtct caccgtggcc
       61 gatctgcagg aggtgatcag tgccgctgtt ggcggaatgt ccctgaccac cacagtggaa
      121 gatcgcgaac gttttccggt acgactgcgt tatccgcgtg agttaagaga gaatccggat
      181 gaattggcac gtctcatcgt gcctactgcc acgggggcgc agattccatt gggcgatgta
      241 acagacataa cctataaacg gggagcgcag atgattcaaa gtgagaatac cttcctgatt
      301 ggatatgtta tcttcgataa ggtagaaggt aaagcggaag tcgatgtggt tcaggaagcc
      361 cagagggtgc ttgatgaaaa aataagaacc ggtgaaatgc aattaccaaa aggagtttcc
      421 tacactttcg ccggaaatta tgaacaacaa gcccgtgcag ctcagcggct ggctattatc
      481 atacccatta gtcttcttgt tatcctgctc attctatatt tccagtttaa aacaatcacg
      541 gcctcgctca ttcacttttc gggtatattt gtcgcgctgg ccggtgggtt cattctctta
      601 tggctctatg gtgaaccctg gtttatgaat tttgatattg gcggaatgaa tatgcgcgac
      661 atgttcaata tgcaacctat taatctgagc attgccgtgt gggttggatt tatagcgctc
      721 ttcggtattg ctacagacga tggggtgctg atgggaacct atattcatga cgcctttctg
      781 gagaaagatc cacagacaag ggaagaagtg agggaaacgg ttgtttatgc cggtaaaaaa
      841 agagtacgac ctgcagtaat gaccacagct actactttga tcgcacttct tccggtgctc
      901 acttccaccg gcaagggttc agagatcatg gtacccatgt caatccctac ttttggcgga
      961 atggtcattc aaatgatgac catgttcatc gtacccatcc tgcagtgttg gtggagagaa
     1021 ggtgttatcc gaaaaaaaga gaaacaacaa gctaaaattg cagattatga aacaatataa
     1081 cgtcaacctc ttattcatca tattgctggg ttttattatt acactcccag tcaaaggcca
     1141 aacggcagat tcactcgccc gataccttga gatagcgggt agaaataatc ccgggttgaa
     1201 tgccgatttc ctggcttaca aagcatcact ggaaaaggta ccacaggcag gtgcatggcc
     1261 agatcccaaa ctggatatcg gcttttttct taaaccgatg gacattgtag gtggtcgaca
     1321 gatagctgat tttaccctta tgcagatgtt cccctggttt ggtaccaaga aggcggcaca
     1381 aaccgaggcc acgcacatgg ccaaaatgtc gtatgaacaa ttcagggaga caagagataa
     1441 cctctacctg gaggtatata cccagtggta tgtcctttct acactggtac agcaactgaa
     1501 taacaaccga gagaacctgc agctgttgaa acaactcgag gaactggcca ttcgcaaggt
     1561 atcatcgcca tacagcggtt cttcttcggg ttattcagta cctgctcctt ctccggcaac
     1621 aaaaagcagc aactcatcac caaccggcgg tggcatgtct gggatgggtt cgatgggagg
     1681 atccacaact tcttccggca cctcctcttc ctcgatgtcc ggcatgagca gttcaggagg
     1741 cagtatgtct tcgggtatgg gcagtactgc cccgggtatg tcggatgtac tccgtgtgca
     1801 actggaaatg gttgaaattg aaaacaacat agagagcctc ttgtcggata ttgcagctga
     1861 aaaagccaga tttaacgcgt tgctgaaccg ttcggtcgat tttgaggtag tggtgcccga
     1921 ttctatcgtg aaagtagact atctcttcga caaagcttct tctatgcgtg aaatagaaaa
     1981 tcaaaacccc atgttgggaa tgctgaaaga agaagaactg gcataccgcg caaagagaga
     2041 gatggacttg
//
LOCUS       DAOSDR010000461         2042 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrimonas sp. isolate AMR_MDS_1826
            NODE_18865_length_2042_cov_1.829391, whole genome shotgun sequence.
ACCESSION   DAOSDR010000461 DAOSDR010000000
VERSION     DAOSDR010000461.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863355
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrimonas sp. (wastewater metagenome)
  ORGANISM  Petrimonas sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Dysgonomonadaceae; Petrimonas.
REFERENCE   1  (bases 1 to 2042)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2042)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:10:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,720
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 35
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2042
                     /organism="Petrimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18865_length_2042_cov_1.829391"
                     /isolate="AMR_MDS_1826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2023866"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..433
                     /locus_tag="PLI30_13285"
     CDS             <1..433
                     /locus_tag="PLI30_13285"
                     /inference="COORDINATES: protein motif:HMM:NF027664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4974 domain-containing protein"
                     /protein_id="HOI80566.1"
                     /translation="ITVYGTSFNVSSYTDDPSESVVLVNGSVEVKSAGNSLMLKPNQK
                     AEISNGSIQQQQVDVMEYTSWKNGYMLLNKTSLTNVLKKIGRYYNIEFNYDAGLKLHN
                     QTCSGKLFLSDNFEDVLQSFSKMTFLNYNVLNERVIYISKP"
     gene            736..>2042
                     /locus_tag="PLI30_13290"
     CDS             736..>2042
                     /locus_tag="PLI30_13290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019539248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HOI80567.1"
                     /translation="MSAELKNVTLREALQEIEKNSDYLFLMMDNTEAALSTSIDCNFI
                     NKSITEILDFLLKKTELTYSIVNRQITISRKPLAEEGKKVIITTTEVMQQTGKTITGR
                     VIDAKGEAIIGANIIEVGTSSNGTITDFDGRFTFRVADDASIHVSYIGYMSQTISTAG
                     RTHFNITLLEDTQSLEELVVVGYGVQTKANLTGAVSTVDVSKTLEARPYSDISKALQG
                     AVPGLSVISNSGKLGAQPSVTIRGLGTLSNDATSKPLIIVDGVPMDDLSLLNTEDIEN
                     ISVLKDAASTSIYGTRAAFGVILITTKSARQVDRVSINYTNNFAWDTPTILPDYPDVP
                     SQLKALIAANERAGLANELFGMYLDEMLPYAEKWRDEHNGKKANYREMVLGDKNALTD
                     SSKPLGDFILDNNGVGMYFADWDVVNIMFRNWKPSQSHNLSLQG"
BASE COUNT          679 a          436 c          424 g          503 t
ORIGIN      
        1 gataaccgtt tacggaactt cgttcaacgt atcttcctat accgatgacc caagtgaatc
       61 ggtggtgctg gtgaacggat ctgtggaagt aaagagcgcg ggcaactctt tgatgctgaa
      121 acctaaccaa aaagctgaaa ttagtaacgg aagtatccag caacaacagg tggatgttat
      181 ggaatatacc agttggaaaa acggatatat gttgttgaac aaaacctcat tgaccaatgt
      241 actcaaaaaa atcggaagat actataacat cgaatttaat tacgatgcag ggctaaaact
      301 gcacaatcaa acctgttcgg ggaaattatt cctgtccgat aattttgaag atgtgttgca
      361 atcattttca aaaatgacct ttctgaatta caacgtactg aatgaaaggg ttatatacat
      421 aagcaaaccc taaaaaacgt aaatttaatt tacatgtatc atttaaaaca aaaagcctat
      481 gataacaatc ccaaaagaaa actaaaaagc cgaataatgc ggtaacatta tccggccaaa
      541 atgtaagtga atcataatct atttggtaac aaattaaaat tcaatgacaa agatatgcaa
      601 gaaaataata accgtgcaca ttttaccacg aaaaaaaccg aaaagttttt tacaatcatg
      661 aaaatttatt ttttgatagc tttactctgt attttttcaa tctcggcaga aaacacctat
      721 tcccaatcaa aaacagtgag tgccgaatta aagaacgtca cgctgaggga ggcattacaa
      781 gaaattgaaa aaaacagcga ttacctgttc ctgatgatgg ataataccga ggcggcattg
      841 tctaccagta ttgattgtaa ctttatcaac aaatccatta cggaaatatt ggattttctc
      901 ctgaagaaaa cagaacttac ctactcgata gtcaaccgtc aaatcacgat ttcacgaaaa
      961 ccgttagccg aggagggtaa aaaagtaatc atcaccacaa cggaagtcat gcaacaaacc
     1021 ggaaagacaa tcactggaag agtgatcgac gccaaaggag aagccatcat cggagccaat
     1081 atcattgaag ttggcacctc ttccaatggg accatcaccg acttcgacgg acggtttact
     1141 ttcagggtag ccgacgatgc gtccattcat gtttcctata ttggttatat gagtcagaca
     1201 atcagcaccg caggaagaac ccatttcaac attaccctgt tggaagatac gcaatcactg
     1261 gaagaactag tagtggtggg gtatggggtt cagacaaaag ccaatttaac aggagctgtc
     1321 tccaccgtgg atgtcagcaa aacgctggag gcacgtcctt attccgacat ctcgaaagcc
     1381 ctgcagggag cagtcccggg cttatccgtt atcagcaaca gtggcaagct gggggcgcaa
     1441 ccctccgtaa ccatacgtgg tttggggaca ttaagcaatg atgccacaag taaacccctg
     1501 atcattgttg atggcgttcc catggacgat ctctcccttc tgaatacgga ggatattgaa
     1561 aacatatctg tcctgaaaga tgctgcctct acttcaatct acgggaccag agccgctttc
     1621 ggagtgatac ttatcactac caaatcggca cggcaggtgg atcgggtatc tataaactat
     1681 accaacaatt ttgcttggga tacccccacc atattacctg actaccccga tgtcccttct
     1741 caattgaaag cactgattgc cgctaatgaa cgcgcaggat tggccaatga actgtttggc
     1801 atgtatctcg atgagatgct tccatatgct gaaaaatgga gagacgaaca taacggcaaa
     1861 aaagccaatt accgggaaat ggtgttgggt gataagaatg cacttacaga cagttcaaaa
     1921 cctctcggcg atttcattct ggacaacaac ggcgtgggca tgtattttgc agactgggat
     1981 gtagtgaaca ttatgttccg aaactggaaa ccgtcacaga gccataacct ctccctgcag
     2041 gg
//
LOCUS       DAOSDR010000462         1962 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrimonas sp. isolate AMR_MDS_1826
            NODE_19852_length_1962_cov_4.192449, whole genome shotgun sequence.
ACCESSION   DAOSDR010000462 DAOSDR010000000
VERSION     DAOSDR010000462.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863355
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrimonas sp. (wastewater metagenome)
  ORGANISM  Petrimonas sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Dysgonomonadaceae; Petrimonas.
REFERENCE   1  (bases 1 to 1962)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1962)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:10:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,720
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 35
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1962
                     /organism="Petrimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19852_length_1962_cov_4.192449"
                     /isolate="AMR_MDS_1826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2023866"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            428..1201
                     /locus_tag="PLI30_13295"
     CDS             428..1201
                     /locus_tag="PLI30_13295"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007665020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOI80568.1"
                     /translation="MSNENISIHDFDFNLICEYFTNLERQGPGSPEVTLKALSFIEPL
                     TDTARIADIGCGTGGQTMTLAQHCPGQITGIDLFPLFIDLFNRNAAKHNLQDRVKGIT
                     GDMASLPFKEEELDLIWSEGSIYNIGFEKGLNEWRKLLKRGGYIAISEASWFTVERPA
                     EIHDFWMDAYPEIDTVSHKVAQMEKAGYVPVATFILPENCWTDHFYAPQVAVQEMFLQ
                     QHAGNPTAEMLVREQRREKSLYDKYKEYYGYVFYIGKKI"
     gene            1260..1676
                     /locus_tag="PLI30_13300"
     CDS             1260..1676
                     /locus_tag="PLI30_13300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006844055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3788 family protein"
                     /protein_id="HOI80569.1"
                     /translation="MQLRDPEIIPTERVLNDVLGNSVYSVLASFLGRITSPEYGLNIE
                     WRYYNDGKAWLGKITQKKKTILWLSIWEGFFKTSFYFTEKHLKSFAELDISKTKKEEF
                     AAMKPIGKLFPLTIDISCKEQLTDLLTVIGFKKSLQ"
     gene            complement(1683..1868)
                     /locus_tag="PLI30_13305"
     CDS             complement(1683..1868)
                     /locus_tag="PLI30_13305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI80570.1"
                     /translation="MTPSELLHIRLYNQLLAMHSLTEPREVVAWMGAMQAQALEMPKK
                     ALSLFQPEVERVEKFYT"
BASE COUNT          572 a          464 c          439 g          487 t
ORIGIN      
        1 tctttccatt tatatgcttg cttcttttcc tcatataccc cattgtttaa aaaagaaagc
       61 tgtataatta cccctgaatc ttagacaaca agtatcgtta aaaaataata ttaattttgt
      121 tgtcatgaag aaaaggaaaa cttaccgtgc tggctttaaa acagaagttg ttcccatttt
      181 ttgaatacca cagcgtgcat tttacaggta ttgcgtccct actcaaaaaa acgattcact
      241 tgattatttt tgataaaaaa cctgttatct ttgtcactgc taacaagttt gcatgtttaa
      301 gaacagtacg gatcgggtga agtaaataac ctttgcgcaa gcagccaaag gttatccatc
      361 agtttttcct caggcagatt cctgccggag tagtatttgt tatatcacca cattattcaa
      421 aaacatcatg agcaacgaaa acatttccat acacgacttc gatttcaacc tgatctgtga
      481 atatttcacc aaccttgaac gacagggacc gggaagtccc gaagtaacgc tgaaggcact
      541 cagcttcatc gaacccctca ccgacactgc ccgcatcgcc gacatcggct gcggcaccgg
      601 ggggcagacc atgacgctgg cacaacactg cccggggcag attaccggca tcgacctctt
      661 ccccctcttt atcgatctct ttaaccggaa tgcggccaaa cacaacctgc aagaccgggt
      721 gaaagggatt actggagata tggcttcact tccattcaag gaggaggagc tggacttgat
      781 ctggtcggaa gggtcgattt acaatattgg ttttgaaaaa ggattgaacg aatggcgaaa
      841 actgctgaag agaggcgggt atattgccat atcggaagcc tcctggttca cggtggaacg
      901 tcctgccgag atccacgatt tctggatgga tgcctatccg gagatcgaca ccgtctccca
      961 caaggtggca cagatggaga aagcaggcta tgttcccgta gctaccttta tcctgccgga
     1021 aaactgctgg accgaccatt tctatgctcc gcaggttgct gtgcaggaga tgttcctgca
     1081 gcagcatgcg ggcaacccca cggccgagat gctcgtccgg gagcaacgtc gcgagaaatc
     1141 gctgtatgac aaatacaagg agtactacgg atatgtcttc tatatcggga agaagatctg
     1201 agattatttg acaaatttta accggcaacg gcttatggac tttggctatg gaaccaaaaa
     1261 tgcaactgag agacccggaa ataattccca cagaaagggt actgaatgac gtattgggca
     1321 attcagtcta cagcgtcctg gcatcgttcc tcggaaggat tacaagcccg gagtatgggc
     1381 tgaacatcga gtggaggtat tacaacgacg ggaaagcatg gctcgggaaa ataacacaaa
     1441 agaaaaaaac cattctctgg ctttccatct gggaaggatt cttcaagacg agtttctatt
     1501 tcaccgaaaa gcatctcaaa tcctttgctg aactggatat ttcgaaaacc aaaaaagagg
     1561 agttcgccgc gatgaaaccg atagggaagt tattccccct gacaattgat atcagctgca
     1621 aggagcaact taccgacctc cttaccgtta tcggcttcaa gaaaagcctt caataagtat
     1681 cattaagtat aaaacttctc cacccgctcc acttccggct gaaaaagaga gagggccttt
     1741 ttgggcatct ccagcgcctg agcctgcatc gcacccatcc aggctaccac ctcccggggc
     1801 tccgtcagag aatgcatagc gagcaactga ttgtaaaggc ggatatggag cagttcggaa
     1861 ggtgtcatac gtttttggtt taaaagacca aatgaccaat ttttttttaa caaacccggt
     1921 tgacaaaaag catatgcgta tctttgtccc gataaaatca gc
//
LOCUS       DAOSDR010000463         1945 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrimonas sp. isolate AMR_MDS_1826
            NODE_20068_length_1945_cov_2.995238, whole genome shotgun sequence.
ACCESSION   DAOSDR010000463 DAOSDR010000000
VERSION     DAOSDR010000463.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863355
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrimonas sp. (wastewater metagenome)
  ORGANISM  Petrimonas sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Dysgonomonadaceae; Petrimonas.
REFERENCE   1  (bases 1 to 1945)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1945)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:10:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,720
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 35
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1945
                     /organism="Petrimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20068_length_1945_cov_2.995238"
                     /isolate="AMR_MDS_1826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2023866"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(122..1753)
                     /gene="pruA"
                     /locus_tag="PLI30_13310"
     CDS             complement(122..1753)
                     /gene="pruA"
                     /locus_tag="PLI30_13310"
                     /EC_number="1.2.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019540278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003842 - 1-pyrroline-5-carboxylate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006562 - proline catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-glutamate gamma-semialdehyde dehydrogenase"
                     /protein_id="HOI80571.1"
                     /translation="MSKGIYKLPEIKNEPVKSYAPGSPERQALKRKLAELNKGGLDIP
                     MIIDGKEVRTGNLYDICPPHDHQRLLGHYHQGDKTHVQMAIDAALKAKPAWEAMHWES
                     RAAIFLKAAELIAGPYRYDFNAVTMIGQSKNAFQSEIDAVCELIDFYRFNVRNMYDLY
                     GMQPNSAPGVWNRTVWRPLEGFVFALTPFNFTSIAANLGGAPALMGNTVVWKPSKTAV
                     YSAHLIMKVLKEAGLPDGVINLVYVGGREAAEIIFNHREFAGLHFTGSTGVFNGMWKT
                     IAGNMEKYKSYPRIVGETGGKDYVFADATANAKQVATALTRGAFEYQGQKCSAASRAY
                     IPTTLWEEVKNHMEEDLKSIKTGTPEDFTHFINAVIDEESFDKLAKEIADAQQSLDAE
                     VIMGGNCDKSKGYFIEPTVILAKKPDYNTMKEELFGPILTVYVYDPEKLDETLDILDT
                     TTDYALTGAIFSSNRANIEKMTSRLTHTAGNFYINDKPTGAVVDQQPFGGGRASGTND
                     KAGSVFNLLRWVSPQTIKETFVPATDYMYPNFLKD"
BASE COUNT          479 a          414 c          433 g          619 t
ORIGIN      
        1 aagcttcgta ttttcctttg gaaatatcga tctgtctgtc gttgactcgc cgatggaaaa
       61 tttccttgta tctggttatt cgattactgc gttgtgttag tgtaacgcag tgttaattag
      121 attaatcctt taggaaattc ggatacatat aatccgttgc cgggacgaaa gtctccttaa
      181 tggtttgcgg agagacccaa cgaagcaggt taaaaacaga gccggcttta tcgtttgttc
      241 ctgatgcgcg gccaccacca aacggctgct ggtcaactac agcgccggta ggtttgtcgt
      301 tgatgtaaaa attaccagcc gtatgagtaa gccgggaagt cattttctct atattggctc
      361 tgttggagga gaaaatagcc cccgtgagtg cataatcggt ggttgtatca agaatatcca
      421 atgtctcatc caatttctcc ggatcgtaaa cataaacggt tagtatagga ccgaaaagtt
      481 cctctttcat cgtgttatag tcgggtttct tggcaagaat aactgtcggt tcaatgaagt
      541 accctttcga cttgtcgcag tttccgccca taattacttc ggcatccagc gattgttgcg
      601 catccgcaat ctcttttgcc agtttatcga aagactcttc atcgataacc gcattgatga
      661 aatgggtgaa atcttcaggg gtacccgttt ttatcgattt taaatcctct tccatatgat
      721 ttttaacctc ttcccacagg gtcgtaggaa tatatgcccg ggaagcggca gaacattttt
      781 gtccctgata ttcgaatgct ccgcgtgtga gggcagtggc tacttgttta gcattggccg
      841 ttgcatcggc aaaaacgtaa tcttttccac cggtttcacc cacgatacgc gggtaggatt
      901 tatatttctc catatttccg gctatggttt tccacattcc attaaagaca cccgtagagc
      961 ccgtaaaatg taatccggca aattcccggt gattaaaaat tatctcggct gcttctcttc
     1021 ctccaacata aactaggttg ataacgccgt cgggtagtcc ggcttctttt aacactttca
     1081 taataagatg agctgaatag accgctgttt tcgatggttt ccagactacg gtgttgccca
     1141 ttaacgccgg tgcccctccc aagttagcgg ctatggacgt gaaattaaag ggagtgagcg
     1201 caaaaacaaa tccttccagc gggcgccaga ccgtgcggtt ccagactccg ggagccgaat
     1261 tgggctgcat accataaagg tcgtacatgt tgcgtacgtt gaaacggtaa aaatcgatta
     1321 attcgcaaac agcatcaatt tcagattgga aggcattttt cgattgtccg atcatggtga
     1381 cggcattaaa gtcgtaccgg tatggtccgg caatcagttc ggctgctttc aggaatatgg
     1441 cagcacggct ttcccagtgc atcgcttccc aggcgggttt tgcttttaga gcagcatcga
     1501 tagccatctg tacgtgtgtt ttgtctcctt gatgatagtg tcccagtaag cgttgatggt
     1561 cgtgtggcgg gcaaatatcg tataagtttc ccgtacggac ttcttttccg tcaatgatca
     1621 ttggaatatc caatccgcct ttatttaatt cggcaagttt ccgttttaaa gcttgtcttt
     1681 ccggtgaccc cggtgcgtaa ctctttacag gttcgttctt aatttcgggt agtttataaa
     1741 ttccttttga cataaggcgt attgtttttt gtgtggttag tattattttt agtgttacaa
     1801 atttataaaa aaaatacgga gaagaagagc agtttgctat tgaattaatt gtaaaattca
     1861 tttttaacac tttctcatgc aacgtttttt cgtttttttc atctattata cgtaacattg
     1921 taattcgttt atgaatcaga tgagg
//
LOCUS       DAOSDR010000464         1925 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrimonas sp. isolate AMR_MDS_1826
            NODE_20347_length_1925_cov_2.689305, whole genome shotgun sequence.
ACCESSION   DAOSDR010000464 DAOSDR010000000
VERSION     DAOSDR010000464.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863355
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrimonas sp. (wastewater metagenome)
  ORGANISM  Petrimonas sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Dysgonomonadaceae; Petrimonas.
REFERENCE   1  (bases 1 to 1925)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1925)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:10:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,720
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 35
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1925
                     /organism="Petrimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20347_length_1925_cov_2.689305"
                     /isolate="AMR_MDS_1826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2023866"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            1..296
                     /locus_tag="PLI30_13315"
                     /pseudo
     CDS             1..296
                     /locus_tag="PLI30_13315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012457950.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(513..1229)
                     /locus_tag="PLI30_13320"
     CDS             complement(513..1229)
                     /locus_tag="PLI30_13320"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009319674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA];
                     GO_process: GO:0098869 - cellular oxidant detoxification
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HOI80572.1"
                     /translation="MEKDQENTLSMPRIGDKAPSFTAKTTQGIINFPEDFSGKWKILF
                     SHPADFTPVCTSEFMTFASMQKDFDALNCQLVGLSVDGLYSHIAWLRTIKDKIEYKGM
                     KNVEVTFPLIEDITMEVAKKYGMIQPNENSTQAVRAVFFVDPKGIIRTIIYYPLSLGR
                     NFDELKRILLGLQTADEFGVALPADWRPGDDVIVPTAGSCGVAKERMEITSEDMHCYD
                     WFFCTKKLSKETVENALLNN"
     gene            complement(1340..>1925)
                     /locus_tag="PLI30_13325"
     CDS             complement(1340..>1925)
                     /locus_tag="PLI30_13325"
                     /inference="COORDINATES: protein motif:HMM:NF012790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HOI80573.1"
                     /translation="NASLDKPGFMNGNLLITKLLVEKRSGKILGAQVIGPGDVSKQVA
                     IWAMAIKGQFNVDDMVNADLPYAPPFSLAIDHSVATAHVMQNKLKGLFRGISAEQLKD
                     KLKNKEPMLLLDVRNPNEFEEVRLGIGERLVPLGQLRNRLGEMPEDKSREIITWCKIS
                     LRGYEAALILQANGYTNVKVLEGGIVAWPWSREK"
BASE COUNT          556 a          423 c          385 g          561 t
ORIGIN      
        1 attatcatta acgacaaagg tgaaattatc agttttgtca ttacccgggg taacgtggat
       61 gaccgccatc cactctcgat ggagagttca ttaaaggagg agaacttccg ttgcaagaca
      121 gggtaatgct tcgcaagaga gccgttattg aatcggtaaa cgatgaattg aagaatatct
      181 gtcagattga acatacacgg cacaggagtt ttacaaactt tatcaccaat ctggtagcag
      241 ggttactggc gtacaacttt ttgccgaaga aaccttctat cagggtagac tttgtagatg
      301 acaaacaact tatgctattt aaaaaatcat atcgaactca cgttaattta atgatgaaca
      361 gcaatttatt ggagttgtac agatcaaaat attcaattgt tatcggtgaa gaaagcaaag
      421 taagattcat atgcagattg tgaaacggaa tcatcataaa aaaatcccgc attctgatga
      481 agcgggatca attccgatat tgccaggtcg gttcagttat tcagtaaggc attttcaact
      541 gtttctttcg aaagtttttt cgtgcagaag aaccaatcat aacagtgcat atcttctgac
      601 gtgatctcca ttctctcttt ggcaacgccg caggaaccgg ccgtaggtac aatcacatca
      661 tcaccgggac gccagtccgc aggaagggcg acaccaaact catcggctgt ctgcaaaccg
      721 agaagaatac gtttcagctc atcgaaatta cggcccaatg acaggggata ataaataatt
      781 gtccggatta ttcctttggg atctacaaaa aagacggcac gcacagcctg agttgagttt
      841 tcattgggct gtatcatacc atactttttg gcaacttcca tcgtgatatc ttcaatcagc
      901 gggaaggtaa cctctacatt tttcattcct ttgtactcta ttttatcctt gatggtacgc
      961 agccatgcaa tatgactgta taaaccatct acggacaaac cgaccaactg acaattcagc
     1021 gcatcgaaat ctttttgcat agaggcaaag gtcatgaact cggatgtgca aacaggcgta
     1081 aaatcagcag gatgactaaa gagtattttc cattttccgg aaaagtcttc cgggaaatta
     1141 ataatgccct gggttgtttt tgcagtaaac gagggcgctt tgtctccaat tcttggcatg
     1201 gagagggtgt tttcttgatc tttttccatt ttaaaataat tttaatgatg actattcgta
     1261 aaaagttatt ggactatgta gcattaaata atctgcgatg cacctatatt ataaccatat
     1321 aaaactgttc ctgcgttaat tacttttcac gtgaccatgg ccaggctact atacccccct
     1381 ccagcacttt cacgttggtg taaccgttgg cctgtagaat cagggctgct tcataaccac
     1441 gcaacgatat tttacaccag gtgattatct cccgggattt atcctcgggc atttctccca
     1501 gtcggttccg aagttgtccc agaggaacca gtctttctcc aatacccaga cgtacctctt
     1561 caaattcatt ggggttgcgt acatccagca aaagcatagg ttctttattt tttagcttat
     1621 ctttcagctg ttctgctgag atacctctaa ataatccttt caatttattc tgcatgacat
     1681 gtgcagtggc aacactgtga tcaatggcca gcgagaaggg tggagcataa ggaagatcgg
     1741 cgttaaccat atcgtccaca ttaaactgac ctttaattgc catcgcccaa atggcgacct
     1801 gtttactgac atcacccggc ccgatcacct gtgcacccaa tattttcccg gatcgctttt
     1861 ctaccaacaa cttggttata agaaggttgc cattcataaa tcccggttta tccaagcttg
     1921 catta
//
LOCUS       DAOSDR010000465         1909 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrimonas sp. isolate AMR_MDS_1826
            NODE_20575_length_1909_cov_4.715750, whole genome shotgun sequence.
ACCESSION   DAOSDR010000465 DAOSDR010000000
VERSION     DAOSDR010000465.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863355
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrimonas sp. (wastewater metagenome)
  ORGANISM  Petrimonas sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Dysgonomonadaceae; Petrimonas.
REFERENCE   1  (bases 1 to 1909)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1909)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:10:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,720
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 35
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1909
                     /organism="Petrimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20575_length_1909_cov_4.715750"
                     /isolate="AMR_MDS_1826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2023866"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(51..1223)
                     /locus_tag="PLI30_13330"
     CDS             complement(51..1223)
                     /locus_tag="PLI30_13330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019538052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro peptidase family protein"
                     /protein_id="HOI80574.1"
                     /translation="MNPINKKESSQRLSRLQQTIRELQAEACLISTTVNQFYLLGFIF
                     DGFVFVQPEGDPVLFVKRPSGIKGDNITYIRKPEQLPDLLHQRKIPLPRRLMMENDVL
                     SFSTVSRIQTALEMPKLINVSGEMRRIRSVKSEFEIKQMRECADIHALVYKQIPHIYS
                     KGMTDIEFQIELEREMRLAGSSGIFRTFGENMDIFMGSILTGDNAQSASPFDFALGGQ
                     GLSPLLPIGANGTKLIPGTTLMVDMAGNYRPWMSDMTRTFAIGTVPDIAHKAHQVSIN
                     ICNAISATSQSGTPCANIYSLAEMMVRESGLSGYFMGTAQQAKFVGHGVGLEINEPPV
                     LAPRSKEILEAGMTIALEPKFVLPGIGAVGIENTYIVRNDRLEKITGCEEELVELS"
     gene            1354..>1909
                     /locus_tag="PLI30_13335"
     CDS             1354..>1909
                     /locus_tag="PLI30_13335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019538051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOI80575.1"
                     /translation="MKNKKPIFTVSIIAQVNGKILIVEDDISFGSMLQKWFEKNGFSA
                     KWCTGMLSAQELLADSTYDIVLSDLRLPDGDGIMLLSWMHERYPSVPVIIMTGYGEIQ
                     TAVSAIKLGAFDFLEKPINPSVLAEKIEAAFKDRKKTENILRKHSQEKKKKEKRGGTV
                     EGCSPLSSRMYSFINTVAPTSMAVM"
BASE COUNT          537 a          420 c          427 g          525 t
ORIGIN      
        1 atttataacc agttgaccag tcaatcaccg gaagaatgat caaccggaat tcatgataac
       61 tccactaatt cttcctcgca accggtaatt ttctccaacc tgtcattgcg aacgatatag
      121 gtattttcta ttcccacagc gccgattccc ggaagtacaa atttgggttc tagggcaatt
      181 gtcatacctg cttccaggat ttccttcgag cggggtgcaa gaacgggcgg ttcattaatc
      241 tccaacccta ctccgtgccc tacaaatttg gcctgttgtg ctgttcccat gaaataaccg
      301 gaaaggccgc tttcccttac catcatttct gccagagaat aaatattggc acaaggtgtt
      361 cccgattggc ttgttgcgga aatggcattg caaatattga tggaaacctg atgagctttg
      421 tgggcaatat cgggtacagt accaatagcg aatgtccggg tcatatcact catccacggg
      481 cggtaattgc ccgccatatc taccatcaac gtagttccgg gaataagttt cgtaccgttg
      541 gctccaattg gcaacaaggg ggataatccc tggccgccaa gtgcgaaatc aaacggggaa
      601 gcggattgag cattgtcacc ggtaagaatg ctgcccataa agatatccat gttctctccg
      661 aaagttctaa aaatacctga cgaaccggct aaacgcattt ctctttccag ctcgatctga
      721 aattcgatat ccgtcatccc tttgctataa atatgcggga tttgtttgta aaccaaagca
      781 tgaatatcgg cgcactcgcg catctgtttg atctcaaact ccgatttaac acttcggata
      841 cggcgcattt ctcccgaaac atttatcagt ttgggcattt caagggctgt ctgaatccgt
      901 gaaacggtgg aaaacgacaa tacatcgttt tccatcatca gcctcctggg caacggaatt
      961 tttctctggt gcagaagatc gggaagctgt tcgggctttc ggatgtaggt aatattgtca
     1021 cctttgatgc cgctggggcg ttttacgaac aaaaccggat ccccttcggg ttggacaaaa
     1081 acgaaaccgt caaaaataaa accaagcagg taaaattggt ttactgtcgt ggaaatcagg
     1141 catgcctcag cttgtaattc ccggatagtt tgttggagcc gggatagtct ttgactggat
     1201 tctttcttat ttataggatt catgaaacaa ctcttttttt gattttcaaa tatagtcaat
     1261 tctcgtgtga acggaaaccc tctttcccaa aaaaacttaa tcttattctg tcaaatgtta
     1321 ggtttcataa atgcatttgc tgacggagaa caaatgaaaa ataaaaaacc tatctttacg
     1381 gtgtcaatta ttgctcaagt gaacggaaaa atattaatag tcgaagacga catctctttt
     1441 ggatcgatgc ttcaaaagtg gtttgagaag aatggattct ccgcaaaatg gtgtacggga
     1501 atgttgtcgg ctcaggaatt gcttgccgat tcaacatacg acattgtctt atcggatttg
     1561 cgcttgcctg acggtgacgg gataatgctt ctttcctgga tgcacgaaag gtatccgtcg
     1621 gtgccggtta taataatgac tggatatggt gaaatacaga cggccgtttc agccataaaa
     1681 ctgggggctt ttgacttcct ggaaaaaccc atcaatccgt ctgttctcgc agagaaaata
     1741 gaggcggctt tcaaggacag aaaaaaaacg gagaacattc taaggaagca ttcgcaggaa
     1801 aaaaagaaga aagagaagcg aggaggtacg gtagaaggat gcagtccgct gtcctcccgt
     1861 atgtactctt ttataaacac cgttgctccc acttcaatgg ccgtgatga
//
LOCUS       DAOSDR010000466         1903 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrimonas sp. isolate AMR_MDS_1826
            NODE_20664_length_1903_cov_2.962662, whole genome shotgun sequence.
ACCESSION   DAOSDR010000466 DAOSDR010000000
VERSION     DAOSDR010000466.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863355
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrimonas sp. (wastewater metagenome)
  ORGANISM  Petrimonas sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Dysgonomonadaceae; Petrimonas.
REFERENCE   1  (bases 1 to 1903)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1903)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:10:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,720
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 35
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1903
                     /organism="Petrimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20664_length_1903_cov_2.962662"
                     /isolate="AMR_MDS_1826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2023866"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..453
                     /locus_tag="PLI30_13340"
     CDS             <1..453
                     /locus_tag="PLI30_13340"
                     /inference="COORDINATES: protein motif:HMM:NF014860.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HOI80576.1"
                     /translation="RSFLAHMASAQGIHVINAIKGIQAHFDYSTYPINMYTTPEIAQI
                     GMTEQKIKEEGYDYKISEFPLSANGKALIENNTEGLIRLLSDKKYGQVLGVQVVAENA
                     TDMIAEAGAYLKVEATVYDVAHTIHAHPTVSEIFFEAGFDAMDKAIHK"
     gene            complement(469..798)
                     /locus_tag="PLI30_13345"
     CDS             complement(469..798)
                     /locus_tag="PLI30_13345"
                     /inference="COORDINATES: protein motif:HMM:NF012790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HOI80577.1"
                     /translation="MAVFSFLKNHRSQIQSLNPADFKTIIAGKDVQLIDVRTAQEYAQ
                     GTINRARLMDVSSMDFEQEIEKLDKSCPVAVFCHSGARSMYAAKVLVKKGFPAVYNLR
                     GGIVFWK"
     gene            complement(894..>1903)
                     /locus_tag="PLI30_13350"
     CDS             complement(894..>1903)
                     /locus_tag="PLI30_13350"
                     /inference="COORDINATES: protein motif:HMM:NF023809.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3656 domain-containing protein"
                     /protein_id="HOI80578.1"
                     /translation="ASSGRSEINFTPNLSKSFNRGFTHYLFNGRQHDIGSFESPKSIG
                     EFVGTVKTVGRNWLSLSTTLTINNGDGLCFMDKDGLNGFRVNRSEGGRIFPAVMPGLS
                     AGTKVYRNYDHDFENWLTKKTAERKIAANIFIREIPTGFALQISDEDNHSYTFSVILE
                     KQTAQKPQQENIRTQLSKTGTTLFSVKSIDIRFSKEWFIPSSLLGEWRKQLISGLLAV
                     RKINYRQERSPIPPTTHAYPEKELTYLGNVSNSNSGSFYLQHRTKILQPAFEQVQQKN
                     VPLMFTKHCIKFALGWCPRETKEKAGFREPFYLINQQNKLKLSFDCRKCEMRVSLENQ
                     Q"
BASE COUNT          486 a          398 c          458 g          561 t
ORIGIN      
        1 agaagttttc tggcacacat ggcttcggct caagggattc acgtcataaa cgccataaaa
       61 gggatacaag cccattttga ttactccact tatccgataa acatgtatac cacgcccgaa
      121 atcgctcaga tcgggatgac ggaacaaaaa attaaggagg agggttacga ttacaaaata
      181 agcgagtttc cgctgtctgc aaatgggaag gcactgattg aaaacaatac ggagggtttg
      241 atccggctgc tttccgataa gaaatacggg caggtcctgg gggttcaggt tgtggctgag
      301 aatgccaccg atatgattgc cgaagccggc gcatacctga aagtcgaagc cacggtttac
      361 gacgtggccc ataccattca cgctcatccc accgtttcgg aaatattttt tgaagccggt
      421 ttcgatgcca tggacaaggc catccataag tgaaagggag agaaaaattt acttccagaa
      481 aacaattcct cccctgaggt tgtaaacggc aggaaatcct tttttaacca ataccttggc
      541 ggcatacatg cttcgtgccc cgctgtggca gaaaacggct acaggacagg atttatcgag
      601 tttttctatt tcttgctcaa aatccatgga ggaaacatcc attaaccggg ctctgtttat
      661 ggtgccttgc gcatattcct gagcagtccg gacgtctata agctgaacat ccttaccggc
      721 gatgattgtt ttgaaatccg cggggttaag ggattggatt tgacttctgt gattttttag
      781 gaaagaaaaa actgccatac ttagaaacta ttttggattt ttttatgtga cgatttttag
      841 tcattttttg acggattcgg gcttttattt tcataatccg aatctacaac cgattattgt
      901 tgattttcaa gagaaacccg catttcgcat ttccggcaat cgaacgagag ttttaatttg
      961 ttctgttgat tgatcagata gaacggctca cgaaaaccgg ctttctcttt ggtctcacgc
     1021 ggacaccaac ccaatgcaaa cttgatgcaa tgtttggtga acatgagtgg aacgtttttt
     1081 tgctgaactt gttcaaatgc gggttgtaaa attttcgtcc ggtgttgtaa gtaaaaagaa
     1141 ccgctgttgc tgttggagac gttacctaag taggtaagct ctttttcggg ataagcgtgt
     1201 gttgtgggtg ggatcgggct gcgttcctgc cggtaattga tttttctgac cgctagtaaa
     1261 cctgaaatca gttgtttacg ccattctcct agcagggagg aggggatgaa ccactccttg
     1321 gaaaaccgga tgtcgatgga ttttacgctg aacagggtag ttcccgtttt ggagagctgg
     1381 gtgcggatat tttcctgttg cggcttttgc gcggtctgct tttctagaat tactgaaaac
     1441 gtgtaggagt ggttgtcctc gtcagagatt tgtaatgcga acccggtagg gatctctcgg
     1501 ataaaaatat tcgcagcaat ttttcgctcc gctgtttttt tggttaacca gttttcaaag
     1561 tcgtgatcgt aatttctata cacctttgtt cctgcgctga ggccgggcat tacggccgga
     1621 aaaatcctgc cgccttcaga gcggtttacg cgaaaaccat ttaacccgtc tttatccata
     1681 aaacacaatc cgtcaccgtt gttaatggtg agtgttgtgc ttaacgaaag ccagtttctg
     1741 cccacggttt ttaccgttcc cacaaattca ccgatggatt tgggagattc gaaagagccg
     1801 atatcgtgtt gcctgccgtt gaaaagatag tgggtaaatc cccggttaaa actctttgat
     1861 aaattgggtg taaaattgat ttcgcttctc cccgatgaag ctt
//
LOCUS       DAOSDR010000467         1902 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrimonas sp. isolate AMR_MDS_1826
            NODE_20678_length_1902_cov_3.654034, whole genome shotgun sequence.
ACCESSION   DAOSDR010000467 DAOSDR010000000
VERSION     DAOSDR010000467.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863355
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrimonas sp. (wastewater metagenome)
  ORGANISM  Petrimonas sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Dysgonomonadaceae; Petrimonas.
REFERENCE   1  (bases 1 to 1902)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1902)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:10:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,720
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 35
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1902
                     /organism="Petrimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20678_length_1902_cov_3.654034"
                     /isolate="AMR_MDS_1826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2023866"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..876
                     /gene="fmt"
                     /locus_tag="PLI30_13355"
     CDS             <1..876
                     /gene="fmt"
                     /locus_tag="PLI30_13355"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019538492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HOI80579.1"
                     /translation="VENGYNVVGVITAPDKPAGRWYKFQPPAVKEYALSAGLPVLQPE
                     NLKNEVFLSELKRLQADIQVVVAFRMLPEVVWSMPPMGTFNLHASLLPQYRGAAPINW
                     ALINGEKETGVTTFFLKHEIDTGEIIFQEKLAIGEDDNAGTLHDHLMMLGARLVLKTI
                     DAVITGTVQSLPQSELIKEEKELRPAPKIHKETCRITWDRPVQEIHNFVRGLSPYPAA
                     WTEFEVGGEKMNFRIFTTKASPEAHQLRPGEIVTDNKTTLRVAAQDGFIDILDIQLSG
                     KRRMKTGDFLNGFSF"
     gene            complement(890..>1902)
                     /locus_tag="PLI30_13360"
     CDS             complement(890..>1902)
                     /locus_tag="PLI30_13360"
                     /inference="COORDINATES: protein motif:HMM:NF016241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3078 domain-containing protein"
                     /protein_id="HOI80580.1"
                     /translation="FVSKTRKNFYREYPDRVKLSVFHLDSLPSSSKDNEVIETFNPFK
                     ELISTETSYSLNAPQVEGVAIKRKYWIYNGEHSFQLSQSYFSPNWHKGGTSNVNIVNN
                     HILRMNYRKDKVRFNNSFEWRLSVFTAPDDTIRKFRIGDDLLRYYGDFGVDAFNKHWS
                     YSTNLDVRSQLFNNYPTNSNDLRSAFLAPLYVNGGVGLKYNLDNRSEKVRHRRVRLDL
                     HLAPLSFNFRYIGNEKVSVARYGIEEGKKHTMDLGSTVTANLIYDFNRYISWNSRLKY
                     FTSYDKVEAEFENTLNMALTNALSTRFFMNLRFDDSVPPDPKFNHLQVTQLLSFGLNY
                     KW"
BASE COUNT          476 a          495 c          497 g          434 t
ORIGIN      
        1 gtcgaaaacg gttacaacgt ggttggcgtc atcaccgcac ccgacaaacc tgccgggaga
       61 tggtacaagt ttcagccccc ggcggtgaaa gagtatgccc tgtcggcagg attacctgtc
      121 cttcaacccg aaaacctgaa gaacgaagtt ttcctgagcg agctgaagcg cctgcaagcg
      181 gacatccagg tggttgtggc cttccggatg ctgcccgagg ttgtctggag catgcctccc
      241 atggggacgt ttaacctgca cgcatcgttg ctgccccagt accggggagc ggctcccatc
      301 aactgggccc ttatcaacgg cgagaaggag acgggcgtca ccaccttctt cctcaagcac
      361 gagatagata ccggagagat aatttttcag gaaaagttgg caatcgggga ggacgacaat
      421 gcgggaaccc tgcacgatca cctgatgatg ctgggtgccc ggctggtatt aaagaccatc
      481 gatgccgtga taaccggaac cgtccaatcc cttccacaaa gtgaattgat aaaagaagaa
      541 aaggaattaa ggccggctcc caaaatccac aaagagacct gccggatcac ctgggacagg
      601 cccgtacagg aaatccacaa cttcgtccgc gggctgtcgc cctacccggc cgcctggacc
      661 gaattcgaag tgggtggcga gaaaatgaat ttcaggatat tcaccaccaa agccagtccg
      721 gaggcacatc aactgcggcc gggagagatt gttacagaca acaaaaccac cctgcgggtt
      781 gccgcacagg atggttttat cgatatcctt gacattcagt tatcggggaa gagacggatg
      841 aaaaccggtg acttcctcaa cggattttcg ttttaaaccc gtaagccacc taccacttgt
      901 agttcagccc gaaactgagc agttgagtaa cctgcaaatg gttgaacttc ggatcgggtg
      961 gaacactatc gtcgaaccgc aggttcatga agaaccgggt agataaggca ttggttaaag
     1021 ccatgttcaa cgtattctcg aactccgcct ccaccttatc gtaactggtg aaatacttca
     1081 gcctcgaatt ccacgagatg taccggttga aatcatagat caggttggcc gtcaccgtgg
     1141 aacccaagtc catggtgtgt ttctttcctt cctcaatccc gtagcgggcc acgctcacct
     1201 tctcgtttcc gatatacctg aagttaaaac ttaagggtgc tagatgcagg tcgagccgaa
     1261 ccctgcggtg ccttactttt tcggaccggt tgtcgaggtt gtacttcaac cccacaccgc
     1321 cgttaacata caacggagcc aggaatgccg aacggaggtc gttggaattc gtcgggtaat
     1381 tgttgaagag ctgcgacctc acatccaggt tggtcgaata agaccagtgt ttgttgaacg
     1441 catccacgcc aaagtcaccg tagtaacgca acaggtcgtc cccgatccgg aatttacgga
     1501 tggtatcgtc gggagccgta aaaacagaaa gcctccattc aaatgaattg ttaaagcgta
     1561 ccttatcctt ccggtagttc atcctgagga tatggttatt gacgatattg acgttgcttg
     1621 tccccccctt gtgccagttc ggagagaaat agctctggga caactggaag gaatgttcgc
     1681 cgttgtagat ccagtatttc cgctttatcg ctaccccttc cacctgggga gcattcaggg
     1741 aatagctcgt ttcggtagag attagctcct tgaaaggatt gaacgtttcg atcacttcgt
     1801 tgtccttgct ggatgacggc agcgagtcca ggtgaaacac ggagagcttc acgcggtcgg
     1861 gatactcccg gtagaagttc ttgcgtgttt tgcttacaaa cg
//
LOCUS       DAOSDR010000468         1897 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrimonas sp. isolate AMR_MDS_1826
            NODE_20749_length_1897_cov_4.938111, whole genome shotgun sequence.
ACCESSION   DAOSDR010000468 DAOSDR010000000
VERSION     DAOSDR010000468.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863355
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrimonas sp. (wastewater metagenome)
  ORGANISM  Petrimonas sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Dysgonomonadaceae; Petrimonas.
REFERENCE   1  (bases 1 to 1897)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1897)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:10:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,720
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 35
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1897
                     /organism="Petrimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20749_length_1897_cov_4.938111"
                     /isolate="AMR_MDS_1826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2023866"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            37..>1897
                     /locus_tag="PLI30_13365"
     CDS             37..>1897
                     /locus_tag="PLI30_13365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019537958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S46 family peptidase"
                     /protein_id="HOI80581.1"
                     /translation="MKKSFLSLILIFTLTTFSLSADEGMWMLHLLKQQKYPEMKKLGL
                     KLRDYDIYNPNGTSIKDAVVQFGGGCTGEVISSQGLVLTNHHCGYGQIQNHSTLEHNY
                     LENGFWAMTKTEELPNPGLTVTFIDKVEEVTDYVKNCLERDKALDKDGILFLSPAYLK
                     SIAKERVGKEFLENNAGADVEIKPFFDGNQYFMFIKKIYSDIRLVGAPPSSIGKFGAD
                     TDNWMWPRHTGDFSLFRIYADKNGNPAPYSPDNVPLKPKRWLTVSTQGANENDFVMIL
                     GFPGTTYKFYTSWEVAERRDIDNRVRINMRRVRQEALLNEMLADPQVNIQYASKYTGS
                     TNAYKSAIGSNWAIDKRNFVMAKKRQQERLIGWAKKKNKTQFLAALDTIQHIVEQRSD
                     LRFRDRMLNEGIVRGIEFANVPVRNADSLIRALRNKDEKKIENFTLLLLNDYRNFANK
                     NYSVEVDKKVAKAMIAEYIRLVPKDKHPDVFTVLHTEFEGDINKYVDNIFENSVFGSE
                     ANLEKFLSHLTPETLVHDPMFSFARSVKKEEAMLKDQQDAFTRAYLIARRSYLDGVLE
                     MDGATAYAPDANLTLRLAYGQLKGYAPRDAVYYEPQTTVEGIMEKEDPGNWEFV"
BASE COUNT          559 a          403 c          454 g          481 t
ORIGIN      
        1 ttgaaataat ttaccctatc aaacaaaaaa ctgaagatga aaaaatcatt tctgtccctg
       61 atcctgattt ttaccctgac tactttctca ctttctgctg atgaaggaat gtggatgttg
      121 cacctgttga agcaacaaaa atatcccgaa atgaaaaaac tgggattgaa attgagagat
      181 tatgatattt acaatccgaa tggcacatcg ataaaagacg cggttgttca gtttggtggc
      241 ggatgcaccg gtgaggtgat ttcttcccag ggactggtat tgaccaatca tcattgcggc
      301 tacggacaga tacagaacca cagtacgctg gaacataatt acctggaaaa cggattctgg
      361 gccatgacga agactgaaga acttcccaat cccggactga cggttacttt tatagataag
      421 gtggaggagg taaccgatta cgtgaagaat tgtcttgaac gggataaggc gctggacaaa
      481 gacggtatcc tgttcctttc tcccgcctat ttgaaaagca ttgccaaaga gagggtgggg
      541 aaagagtttc tggaaaataa cgctggtgcc gatgttgaga ttaagccatt cttcgacggg
      601 aaccaatatt ttatgttcat caaaaagata tactccgata ttcgtctggt gggggcgccg
      661 cccagttcga tcggaaagtt tggtgccgat accgataact ggatgtggcc acgtcacaca
      721 ggcgactttt cccttttccg catttatgct gataaaaacg gaaaccccgc tccttattct
      781 cccgataatg ttccactgaa acccaagcgc tggcttaccg tttctaccca gggcgcaaat
      841 gaaaatgatt ttgttatgat cctggggttt cccggtacaa cctataagtt ttatacctcg
      901 tgggaagttg ccgagcggcg cgatatcgat aacagagtgc gtatcaacat gcgcagggtc
      961 cgccaggaag ccctgttgaa tgaaatgctt gccgatccgc aggtaaatat acagtatgct
     1021 tcgaaataca caggctctac gaacgcctac aagagcgcta tcggctcgaa ttgggccatt
     1081 gacaagcgta attttgtcat ggcaaaaaaa cgacaacagg aaagacttat cggttgggca
     1141 aagaaaaaga acaaaacgca gttcctggcc gctcttgata ccatacaaca tattgttgag
     1201 caacgcagtg acctccgttt cagggaccgg atgctgaacg aagggattgt cagagggatt
     1261 gagtttgcta atgtgccggt ccggaatgct gactcattga tccgggcact tcggaacaag
     1321 gacgaaaaga agatcgagaa tttcacgtta ctgcttctta atgattaccg gaattttgcc
     1381 aacaagaact acagcgtaga agtggataaa aaagtagcca aagccatgat tgctgaatac
     1441 atacggttgg ttcccaaaga taagcatcct gatgttttta ccgttttaca cactgagttt
     1501 gagggagaca taaacaaata cgtagataat atttttgaaa attctgtttt cgggagtgaa
     1561 gctaacctgg aaaaattcct ttctcatctt actcccgaaa cacttgtcca tgatccgatg
     1621 tttagctttg cccggtcggt aaagaaagaa gaagcaatgt taaaagatca gcaagatgct
     1681 tttacccgcg cctatttgat cgcccgccgt tcatacctgg acggagtgct ggagatggac
     1741 ggagcaacgg catatgctcc cgatgccaat cttacgctca ggcttgcata cggacagttg
     1801 aaaggttatg caccccgtga tgctgtttac tatgaacccc aaacaacggt ggaaggaatt
     1861 atggaaaaag aagatcccgg gaattgggag ttcgttg
//
LOCUS       DAOSDR010000469         1893 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrimonas sp. isolate AMR_MDS_1826
            NODE_20818_length_1893_cov_2.949946, whole genome shotgun sequence.
ACCESSION   DAOSDR010000469 DAOSDR010000000
VERSION     DAOSDR010000469.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863355
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrimonas sp. (wastewater metagenome)
  ORGANISM  Petrimonas sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Dysgonomonadaceae; Petrimonas.
REFERENCE   1  (bases 1 to 1893)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1893)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:10:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,720
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 35
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1893
                     /organism="Petrimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20818_length_1893_cov_2.949946"
                     /isolate="AMR_MDS_1826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2023866"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(207..809)
                     /locus_tag="PLI30_13370"
     CDS             complement(207..809)
                     /locus_tag="PLI30_13370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI80582.1"
                     /translation="MSAESQAWIGFRLNEMMGELVFLFLLIPFFSIGQKHCWGDQPCG
                     QYFRNPGVDILGGNKNFCIIAPDSSTSLLNSEFPGIVQRLWMMRKSSDSLLLRTSVFS
                     FSFEEGELVSSLSPDRLLGTGFHSTNELFFFCGASAIRSFRNAVVAFSYRLQRKHRCP
                     IAVWCGQRKPSGKLVVAVQQLYWEFGRCAPVWRILSGEKG"
     gene            complement(1197..>1893)
                     /locus_tag="PLI30_13375"
     CDS             complement(1197..>1893)
                     /locus_tag="PLI30_13375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI80583.1"
                     /translation="RAGLFASKYYLDHGNYQRSEEIARKYFEINTKNYYLGLQYAKIL
                     ELNRKYDACIKILGKIWILPNEGATEGRIIWRNANIGKALEYFEKTSYRKALESIHNS
                     KKWPANLGVGKPFEVDERFEDFISMLCLQKTGNQTSAKELRDRIIHQPPQQMVSDDTN
                     DFLTAWLWKESGNKSKGDSLMNHLIKRNLSSVKRIWYQAFYSGELQEVEKNVGEPGRT
                     DQLFFFLRWLIKV"
BASE COUNT          561 a          430 c          325 g          577 t
ORIGIN      
        1 aggtccggct tcggtgggaa aaccagcgat tttgtaaaaa gcaatcggaa cggccagatg
       61 gaagcgacac ctgccgaaac gttctccccc agagtggtca ttggaagcaa tgtctctttc
      121 atagaggaca acattagtgt cacaagttgg gcaggcacaa taaatttccc acaccacact
      181 caaattattt tccacttccg cgccatttac cctttctcgc cggataaaat ccgccaaacc
      241 ggagcgcacc gaccaaattc ccaataaagc tgttgaacgg caacaaccaa ttttcctgat
      301 ggttttcttt gaccacacca gacggcaatt gggcacctat gtttacgttg cagccggtat
      361 gaaaaagcca cgaccgcatt tcgaaatgat cggattgcag acgccccaca aaaaaaaaac
      421 agctcgtttg tgctatggaa gccggtaccc agtaacctat cgggcgaaag gctggatacc
      481 agctctccct cctcaaacga aaaactaaaa acggatgttc gcagtaacaa tgaatcgctg
      541 gacttacgca tcatccaaag gcgttgcacg atacctggaa attccgaatt gagcaacgag
      601 gtagagctgt cgggagcaat aatgcaaaaa ttcttattac ctcccaaaat atctacaccc
      661 ggatttcgga aatattgtcc acaaggttgg tccccccaac aatgcttctg accaatcgaa
      721 aaaaatggaa ttaacaaaaa cagaaagact aactccccca tcatctcgtt cagcctgaac
      781 ccgatccatg cttggctttc tgcactcatt tttattatgt ggttgtacaa actgaactct
      841 agccggctgt tttttcttac ttttcaaatg tatctttttt ctggaagatt ttacttttta
      901 aaacgttttg aacaaaagta ggcaaggaca acccgtttga gaatctcctt gaggtcacta
      961 taaaaattca catatcgtcc atctcttcaa aaagatgcaa tatccctgac tcatcacgaa
     1021 tctataaaaa tcaaaaattt tagagcaagg ttttctgcaa atacagttcc ccttagttga
     1081 tctcttaaac aaataaaata ttatatatca acttaatttt tttgcaattc ttcatcctcg
     1141 gtcttttatt aaattataaa gctaaatatc ggtgttaacc aaacgtttat ttgttattag
     1201 actttaatta gccatctcaa gaaaaaaaat aattggtcag ttcggcctgg ttctcctaca
     1261 tttttttcta cctcctgtaa ttcgcctgaa taaaaagctt ggtaccatat tctcttcacg
     1321 gaggaaagat tccgtttaat caagtgattc atcagagaat ctcctttgga cttgttgcct
     1381 gattctttcc aaagccatgc agtcaggaag tcgttagtgt catctgaaac catttgttgt
     1441 ggaggctggt ggataattct gtcgcgtaac tcttttgcag atgtttggtt tccagttttt
     1501 tgaagacaca gcatagagat aaaatcttca aatcgttcat caacctcaaa aggcttacct
     1561 actccgaggt tagcaggcca tttttttgag ttgtgaatgc tttccaaagc ttttctatag
     1621 ctagttttct caaaatattc caaagctttt ccaatattag cgtttcgcca gattattcgc
     1681 ccttctgtag ctccttcatt cggtaaaatc catattttcc caagtatttt aatgcaagca
     1741 tcatattttc tatttaattc cagaattttc gcatactgca acccgagata atagttttta
     1801 gtatttatct caaaatattt cctggcaatt tcttctgatc tctgataatt tccgtggtcg
     1861 agataatatt tcgatgcaaa taatccggct ctc
//
LOCUS       DAOSDR010000470         1885 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrimonas sp. isolate AMR_MDS_1826
            NODE_20926_length_1885_cov_4.331694, whole genome shotgun sequence.
ACCESSION   DAOSDR010000470 DAOSDR010000000
VERSION     DAOSDR010000470.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863355
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrimonas sp. (wastewater metagenome)
  ORGANISM  Petrimonas sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Dysgonomonadaceae; Petrimonas.
REFERENCE   1  (bases 1 to 1885)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1885)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:10:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,720
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 35
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1885
                     /organism="Petrimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20926_length_1885_cov_4.331694"
                     /isolate="AMR_MDS_1826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2023866"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..180)
                     /locus_tag="PLI30_13380"
     CDS             complement(<1..180)
                     /locus_tag="PLI30_13380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI80584.1"
                     /translation="MKKTVVLFYLISIANLIQAQIVWNIGEKDKNTAGFALAPDKYAD
                     FLKNDFGWEDKYFIIG"
     gene            complement(221..1312)
                     /locus_tag="PLI30_13385"
     CDS             complement(221..1312)
                     /locus_tag="PLI30_13385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004326178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HOI80585.1"
                     /translation="MFSNDPRTVLTLDAGGTNFVFTAIRGNKEITDPVILPSHPDNRE
                     VCLETIVNGFSTIKKKLDDLPVAISFAFPGPADYENGIIGDLPNFPAFRGGVALGPFL
                     IEKFGLPVFINNDGDLFAYGEALAGALPEINAELKKAGSSKQYKNLLGITLGTGFGGG
                     VVINNVLLSGDNGCGGDVWCSANKYNNGLVSEEGVSIRAVKRVYRELSGDDTELTPKE
                     IYHIAEVERPGNRKAAIGTFEELGRVAGFTVAESLNIVDGIVVIGGGIAKAYKYILPA
                     MVDEMNGTRSMLSGSVFPRLQMRVYNLMSDTEKKAFLMDESVWVSIPGSDKKMSHNTG
                     KKTGVTVSSLGTSEAIALGAYAYALRMLD"
     gene            complement(1302..>1885)
                     /locus_tag="PLI30_13390"
     CDS             complement(1302..>1885)
                     /locus_tag="PLI30_13390"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOI80586.1"
                     /translation="TPATFSGALGLLKDKFIFFCFLSIFLIVGFDVGLNVSIPELLMR
                     HAGLTLENAGYGNSVYFAAKTTGAFLGTFLLLKIKPMKYLLVSLFVALLSYLALMLIH
                     EVWSLRVLIFMLGFAGANVFSIIFSFALQRFPSRSNEISALMIMGVVGGAVIPFVQGL
                     INDSISFTAALSVIFASLLLILIFTFNLKKNVQ"
BASE COUNT          562 a          448 c          374 g          501 t
ORIGIN      
        1 ccctatgatg aagtatttat cttcccaacc gaaatcattt ttcaggaagt cggcgtactt
       61 gtccggggcc aatgcgaatc cggctgtatt cttatctttt tccccgatat tccagacgat
      121 ttgagcctgg ataaggtttg caattgaaat cagataaaat aaaacaactg tttttttcat
      181 ttggaaatta ttaaagggta tagaaacaga tagtaaatag ttaatccaac atcctcaggg
      241 cataggcata agcccctaaa gcaatggctt cgctggtccc taagctactt acagttacgc
      301 ctgtcttttt acctgtgtta tgactcattt ttttatctga accgggaatg gagacccaaa
      361 cagactcatc catcaaaaag gctttctttt ccgtatcgct cataaggtta tataccctca
      421 tctggagacg tggaaagaca ctaccgctta gcatgctgcg cgttccgttc atttcatcta
      481 ccattgccgg caaaatatat ttgtatgctt tggcgatccc tcctcctatt accacaatac
      541 catccacaat gttgagcgat tcggcaacgg tgaatccggc taccctgcca agctcttcga
      601 aggtgccgat agctgctttc ctattgcctg gtctttctac ctccgctata tgatagattt
      661 ctttgggtgt gagttcagta tcatctcccg acagttcccg atatactctt ttaacggcac
      721 gaatgctgac tccttcttcc gataccaagc cgttgttgta tttgtttgcg gaacaccata
      781 catcgccccc acaaccgttg tcgcccgaaa gcaatacatt gtttataacc accccgccgc
      841 caaagcctgt tcctaaggta attccaagca ggtttttata ttgcttggag cttccggcct
      901 ttttcaactc ggcgtttatt tcgggcaacg ctccagcgag ggcttcacca taagcaaaaa
      961 ggtccccatc gttgttgata aaaacaggca acccgaattt ctcaatcaag aaagggccta
     1021 atgccacacc gccacgaaat gcggggaaat tgggcaaatc acctattatc ccgttttcgt
     1081 aatccgcagg gcccggaaat gcaaagctga tggcaacagg cagatcatcc agcttttttt
     1141 ttattgtgct gaaaccgtta acaatcgtct ccaggcaaac ctcccggtta tcgggatgag
     1201 aaggtaatat aacaggatct gttatctcct tattgccgcg gattgccgta aagacaaagt
     1261 tggtgccgcc ggcatccaac gtcaaaactg ttcgtggatc gttactgaac atttttcttc
     1321 aggttaaagg tgaaaatcag gataagtaaa agagaggcaa aaatgacgga aagtgcggcg
     1381 gtaaagctga tactgtcatt tatcagaccc tggacaaagg ggataacagc accacccaca
     1441 actcccataa tcatcagggc agatatctcg tttgagcgtg agggaaaccg ttgtaatgca
     1501 aaagagaaaa taatggaaaa aacattggca ccggcaaagc ccagcataaa aatcaacacc
     1561 ctcaaactcc acacctcgtg tatgagcatt agagctaaat aagatagtag tgccacaaaa
     1621 agtgaaacga gcaggtactt catcggcttt attttcaaca gaaggaacgt gcccaaaaaa
     1681 gcaccagttg ttttagcagc aaaatagaca ctgtttccat aaccggcatt ttcgagcgta
     1741 agtcctgcat gtctcatcaa aagttcagga atagagacat ttaatcctac atcaaatcca
     1801 acgattaaaa aaatagataa aaaacaaaag aaaataaatt tatcttttaa taatcccaat
     1861 gcgccgctaa atgtagcagg agtgc
//
LOCUS       DAOSDR010000471         1884 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrimonas sp. isolate AMR_MDS_1826
            NODE_20941_length_1884_cov_4.931657, whole genome shotgun sequence.
ACCESSION   DAOSDR010000471 DAOSDR010000000
VERSION     DAOSDR010000471.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863355
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrimonas sp. (wastewater metagenome)
  ORGANISM  Petrimonas sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Dysgonomonadaceae; Petrimonas.
REFERENCE   1  (bases 1 to 1884)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1884)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:10:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,720
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 35
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1884
                     /organism="Petrimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20941_length_1884_cov_4.931657"
                     /isolate="AMR_MDS_1826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2023866"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..480
                     /locus_tag="PLI30_13395"
     CDS             <1..480
                     /locus_tag="PLI30_13395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI80587.1"
                     /translation="PDRGALVAFQREVASLTRVVQGTQRYMNELSDKLAIMMQAVHQT
                     PEASPELKSHMYQLSLQMDDIRYSFYGKTPKASREENPPAPVSINERMGNLIYAFYGT
                     TSAPTTTMRRSYEIIREEFPPLHAKLKQIGTVDIPALETEMEKAGVPWTPGRLPEWE"
     gene            576..1337
                     /locus_tag="PLI30_13400"
     CDS             576..1337
                     /locus_tag="PLI30_13400"
                     /inference="COORDINATES: protein motif:HMM:NF013782.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase"
                     /protein_id="HOI80588.1"
                     /translation="MLNQNLPTQAGELILSMPFSITSAETDMYGRAKLSALINLLIQS
                     SIQSADRLGFGYIDFRPQKLFWVLSRFSVEIAGPLRWMQTGEVETWPKDIDKLLYIRD
                     FIVRDNREHTLAKATSGWLTVDMETKRSKLIDLEDPSLFFRLKDRHALTDRPEKLGPV
                     MGEAVSVLKVSYFDLDINQHVTTTRYFDWMMDTFSHEFHRSRYPKTASINFLKEIRPG
                     ETIEIFRCETSENNFAFEGLSKSRNLTAFRGRITF"
     gene            complement(1357..>1884)
                     /locus_tag="PLI30_13405"
     CDS             complement(1357..>1884)
                     /locus_tag="PLI30_13405"
                     /inference="COORDINATES: protein motif:HMM:NF020893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HOI80589.1"
                     /translation="IPPAAWKAAQGDLNIFMVVITGTVGALIGSLVNYYISLSLGRKL
                     VYAFADTRLARLLLITPEKIQHAESYFVKHGKSSTFIGRLVPGVRQLISIPAGLARMP
                     IRDFILYTFLGAGIWNVVLAAMGYFLYSQKETLELYYHELSYAMLGLGIIFVGYLIYS
                     GRRKKRIAASNKSAL"
BASE COUNT          546 a          478 c          460 g          400 t
ORIGIN      
        1 cccgaccgcg gggcactggt agccttccag cgcgaggtgg caagccttac ccgcgtggtt
       61 cagggtacac agcgctacat gaacgagctg tccgacaaac tcgcaatcat gatgcaggct
      121 gtacaccaaa cgcccgaagc ctcaccggaa ctgaagtcac acatgtatca gctttccctg
      181 caaatggacg atatccgcta cagcttctac ggcaaaacac ccaaggcaag ccgggaagag
      241 aatcctcctg ctccggtttc catcaacgag cgcatgggca acctcatcta tgccttctac
      301 ggaacaacct cagcaccaac caccactatg cgcaggagtt atgagatcat ccgcgaggag
      361 tttccacctc tgcacgcaaa actgaaacaa atcggaaccg tcgatattcc tgcactggaa
      421 accgaaatgg agaaagccgg cgttccatgg accccgggca gattgccgga atgggaataa
      481 ttgcgaatta aaccggtata ataaaaaacc acggcccggg agacaaacaa gccgggctgt
      541 ggtttgataa ctgagacacc gaatcctttt tcatcatgct gaaccagaac cttccaacgc
      601 aagccggcga acttattctt tccatgccgt tttccattac ttcggccgaa accgatatgt
      661 acggcagggc gaaactgagc gcactaatca acctgctgat acagtcatcc atacaatcgg
      721 ccgaccggct tggatttggc tacatcgatt tccgtccgca aaaactcttc tgggtattaa
      781 gccgcttctc ggttgaaatc gccggcccgc tgcgatggat gcaaacagga gaggtggaga
      841 catggccaaa ggacatagat aaactgctct atatccgtga ttttatcgtt cgcgacaatc
      901 gtgaacacac ccttgcgaag gcaacatcgg gctggctgac ggtggatatg gagacaaaac
      961 gcagcaaact gatcgatctg gaggatcctt cgctgttttt ccggctgaaa gaccggcatg
     1021 cactaacgga caggccggag aaactgggtc cggtaatggg tgaagctgta tctgtgctaa
     1081 aggtttccta cttcgaccta gacatcaacc agcatgtaac tactaccagg tattttgact
     1141 ggatgatgga cacattttca catgaatttc accgaagtcg atatccgaaa acggcctcta
     1201 taaatttcct gaaggagatc agacccggag aaacgataga aatattccgt tgcgaaacat
     1261 cggaaaataa ttttgcgttt gaggggttaa gcaagagccg gaacctgacc gcattccgtg
     1321 gccggataac cttctgaagg aaaaaaaagg ataaaattac aaagccgatt tgtttgaggc
     1381 ggcaatacgt ttcttgcgcc ttcccgaata gatcagatat cctacgaaaa ttatcccaag
     1441 tcccagcatg gcataggaaa gctcgtggta ataaagttcc agcgtttcct tctgggaata
     1501 gaggaaatag cccattgcag caagtaccac gttccagatt ccggcaccga ggaaggtata
     1561 gaggataaaa tcgcggatcg gcatacgtgc tagtccggca gggatagaga tcagctgacg
     1621 cacacccgga acaagccggc caataaatgt ggagcttttc ccgtgtttaa caaaataact
     1681 ctcggcatgc tgaatttttt ccggtgtgat gaggagcagt cgggccagac gcgtatcggc
     1741 aaaagcataa acaagtttac ggccaaggga aagggagata taataattaa ccagtgatcc
     1801 gataagtgcc ccaacagtac ctgtgattac aaccataaaa atattcagat cgccctgagc
     1861 agctttccag gctgcaggcg gaat
//
LOCUS       DAOSDR010000472         1880 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrimonas sp. isolate AMR_MDS_1826
            NODE_20996_length_1880_cov_3.301370, whole genome shotgun sequence.
ACCESSION   DAOSDR010000472 DAOSDR010000000
VERSION     DAOSDR010000472.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863355
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrimonas sp. (wastewater metagenome)
  ORGANISM  Petrimonas sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Dysgonomonadaceae; Petrimonas.
REFERENCE   1  (bases 1 to 1880)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1880)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:10:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,720
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 35
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1880
                     /organism="Petrimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20996_length_1880_cov_3.301370"
                     /isolate="AMR_MDS_1826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2023866"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1880
                     /locus_tag="PLI30_13410"
     CDS             <1..>1880
                     /locus_tag="PLI30_13410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006841700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit A"
                     /protein_id="HOI80590.1"
                     /translation="FYRIDELLAQKTIPDIQDVLLVIDRLSCSGDKSVVNRLSDSVET
                     AFIEGKGECIVRFWTQDGAETFTFSNRFEADGITFEEPSDLMFNFNSPVGACPKCEGF
                     GKVIGIDENLVIPDKSLSVQEECVQCWKGEKMSEWRKEFVFNAYKVDFPIHRAYYGLN
                     DEEKDILWNGRHDLGIYGINDFFRMLEENLYKIQYRVMLARYRGKTTCPVCKGARLKK
                     EAGFVKVGGKNISELVLLPVTELKLFFDTLILNETDAAIAERLLTEINNRILFLIEVG
                     LGYLTLNRLSNTLSGGESQRINLASSLGSSLVGSLYILDEPSIGLHSRDTHLLIKVLR
                     QLQQLGNTVVVVEHDEEIIRAADYIIDIGPEAGRLGGRIMYQGNVSELVKNTGSHTVR
                     YLTGEEKIDVPKHRRKWNNFIEVKGARQNNLKNIDVRFPLNVMTVVTGVSGSGKSSLV
                     NDVLSNALHNYYKGSALEQTEFNAISGDLKLAQSVEFVDQNPIGRSTRSNPVTYIKAY
                     DEIRKLFAAQPLAKQMGYTPAHFSFNVEGGRCDECKGEGTITVEMQFMADIRLECESC
                     HGKRFSREILDVEYKGLNIHEVLEMTINQAIEFFGAQKGATEKKITKRMQPLQDVGLG
                     YI"
BASE COUNT          541 a          403 c          500 g          436 t
ORIGIN      
        1 ttttatcgga tcgacgaact cttggcacaa aagacaatac cggatattca ggatgtcctg
       61 ttggttattg accggttgtc ctgttcgggc gacaaatcgg tcgtcaaccg gctgtcggat
      121 tcggtagaaa ccgcttttat agaagggaag ggcgagtgta tcgtccgttt ctggacacaa
      181 gacggtgcag aaacttttac tttttcgaac cgtttcgaag cggatgggat cacgttcgaa
      241 gaaccgtcgg acctgatgtt caacttcaac agcccggtag gtgcctgtcc caaatgcgag
      301 ggtttcggaa aagtaattgg aattgacgaa aacctggtta tccccgacaa gagcctctcg
      361 gttcaggagg agtgcgtaca atgctggaaa ggcgaaaaaa tgagtgaatg gcgcaaagaa
      421 ttcgttttca acgcttataa agtcgatttt cccattcaca gggcatatta cggattgaac
      481 gatgaagaga aggacatctt atggaacggg cgtcatgatc tcggcattta cgggatcaac
      541 gattttttcc ggatgctgga agagaatctt tataaaattc agtaccgggt gatgctggcg
      601 cggtatcgcg gaaaaaccac ctgtccggtt tgcaaaggtg cccggctgaa aaaagaggcc
      661 ggattcgtga aggtaggcgg gaaaaatata agcgaactgg ttctcttgcc cgttacggag
      721 ttaaagttgt tttttgacac acttatactc aatgaaaccg atgccgccat tgctgaacgg
      781 ttgcttacgg agatcaacaa ccgtatcctg ttcctgattg aggtaggact tggttacctg
      841 acactgaacc ggttatcaaa caccctttcg ggaggagaaa gccagcgaat aaacctggcc
      901 tcatccctgg gaagtagcct ggttggatca ctctatattt tggacgaacc gagtattggg
      961 ctgcactccc gggataccca cctgctcatt aaagtgttac gccagctgca gcaactgggc
     1021 aataccgtgg tagtggtgga acatgacgaa gagattatcc gggcagcaga ctatattatt
     1081 gatatcggcc ccgaagcggg gcgactaggt ggaagaatca tgtatcaggg caatgtatcg
     1141 gagttggtga aaaacaccgg cagccatacg gtgaggtacc tgacggggga agaaaagatt
     1201 gatgttccga aacaccgccg caaatggaac aactttatcg aagtgaaggg tgcacgacaa
     1261 aacaacctta aaaacataga tgtgagattt ccgttgaatg tcatgaccgt ggtaacaggt
     1321 gtcagcggat cgggaaaatc ttcgctggta aacgatgtgc taagcaacgc tttacacaat
     1381 tactacaaag gaagtgcatt ggaacaaact gaatttaacg ccatttccgg cgatttaaaa
     1441 cttgcccaat cggtggaatt tgtcgatcag aaccccatag gcagatccac gcgctccaac
     1501 ccggtcacct acataaaggc ttacgacgaa atccgcaagc tatttgccgc tcaaccgctt
     1561 gccaaacaga tgggatatac tccggcacat ttctcgttca atgtagaagg cggaaggtgc
     1621 gacgagtgca agggcgaagg tacaattaca gtggagatgc agtttatggc cgatatccgg
     1681 ctggaatgtg aatcgtgtca cggcaagcgc ttttctcggg aaattctgga tgttgaatac
     1741 aagggattaa acatccacga agtgctggaa atgaccatca atcaggcaat cgagtttttc
     1801 ggggcacaga aaggcgcaac ggaaaagaaa atcaccaaaa ggatgcaacc gctacaagat
     1861 gtagggttgg gctatatcaa
//
LOCUS       DAOSDR010000473         1874 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrimonas sp. isolate AMR_MDS_1826
            NODE_21082_length_1874_cov_3.944475, whole genome shotgun sequence.
ACCESSION   DAOSDR010000473 DAOSDR010000000
VERSION     DAOSDR010000473.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863355
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrimonas sp. (wastewater metagenome)
  ORGANISM  Petrimonas sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Dysgonomonadaceae; Petrimonas.
REFERENCE   1  (bases 1 to 1874)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1874)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:10:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,720
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 35
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1874
                     /organism="Petrimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21082_length_1874_cov_3.944475"
                     /isolate="AMR_MDS_1826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2023866"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..542)
                     /locus_tag="PLI30_13415"
     CDS             complement(<1..542)
                     /locus_tag="PLI30_13415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI80591.1"
                     /translation="MKQTQKFELDPGHVAGHQQFDFNPSEWVPFKDKKEINRVLAIKR
                     EDIERHENPDFKIKVLPDSDVEYYWITDMFRRIKHSSDTGEKLVMILPNPAPLYRHLA
                     RLINACNIDCRNVYAFAMDEYADENGNIAPEGWEFGFTHAMMKYFYYELDKRLRPPKE
                     QFVGFTNKNVHDYSKMIADRG"
     gene            complement(544..1713)
                     /locus_tag="PLI30_13420"
     CDS             complement(544..1713)
                     /locus_tag="PLI30_13420"
                     /inference="COORDINATES: protein motif:HMM:NF014081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HOI80592.1"
                     /translation="MQRNNQIAFKGIIISDSELVKGYLTTSGGKIIDIGESPPGCDVI
                     DLGESYITPGFIDLHIHGIHRMLVDNGAEDLKEICKILPQYGVTGFLPTLAPRPKGED
                     AEFLSELSKTETEGTEVLGFHLEGPFLKITGALGAGAISGADEGRVHALIHAAKPHKA
                     IFSISPDVVGIEKLLPLMAANGTPVFMTHTAATVEQTQLAIELGVRHATHFYDVFPCP
                     SVSEPGVRPCGAVEAILADERVSVDFILDGVHVDPVAAKMSLISKRKGPGKVCLVTDA
                     NVGAGLEPGQFSFGNLGDICFAYKGAPARSVKDNTLAGSGLTMDRALKNAVKWLDLDL
                     VQASKLVSHHPAQVLGLGDKKGLLKEGYDADFVVLDENLDVLRTWINGKCFFERK"
BASE COUNT          467 a          475 c          383 g          549 t
ORIGIN      
        1 ccacggtcag caatcatttt actgtaatca tgtacattct tgttggtaaa tccgacaaac
       61 tgctcttttg gcggcctcag ccttttatcc agttcataat agaaatattt catcatcgcg
      121 tgagtaaaac caaattccca cccttcaggc gcaatatttc cgttttcatc ggcgtattca
      181 tccatggcaa aagcatagac attccggcag tctatattac aagcattgat taatctggcc
      241 agatgtctgt aaagcggggc aggattgggg agtatcatta cgagtttttc cccggtatcc
      301 gaagaatgct tgattcttct gaacatgtcc gttatccaat aatattcgac atctgaatca
      361 ggtaaaacct taattttaaa atcagggttt tcgtgtctct ctatatcctc tcttttgatg
      421 gctaacaccc tgttgatttc ttttttgtcc ttaaagggta cccattctga gggattgaag
      481 tcgaattgct gatgccctgc cacatgccca gggtcaagct caaatttctg agtttgtttc
      541 atattatttc ctttcaaaaa aacatttacc gtttatccat gttcgtaaaa catccagatt
      601 ttcatctaat acgacgaaat cggcgtcgta accttccttt aataatcctt tcttatcacc
      661 gaggccaaga acctgagccg gatgatggct taccaacttt gatgcttgca ccaaatccag
      721 atccagccat ttcacggcat tttttaaagc ccggtccatt gtcaggccac tccctgccaa
      781 tgtgttgtcc tttacggaac gtgcaggagc gcctttgtac gcaaagcaga tatctcccaa
      841 attgccgaac gagaattgtc cgggctctaa tcccgcacct acgttggcat ctgtaacgag
      901 acagaccttg cccggccctt tccttttact gatcaaagac atttttgccg ccacggggtc
      961 aacatgtacg ccatccagga tgaaatccac acttacccgc tcgtctgcca ggatggcttc
     1021 aacggctccg caaggtcgca caccgggttc ggaaacagag ggacagggga atacatcgta
     1081 gaaatgagta gcgtgtcgaa cgcccaattc tatggctagc tgagtttgtt caactgtggc
     1141 ggcagtgtgt gtcataaaga caggggtgcc gttggctgcc atcaacggca acagtttctc
     1201 gattccgact acgtccggag aaatgctgaa aatcgcttta tgtggtttcg ccgcatgaat
     1261 cagagcatgt actcttcctt catctgcccc ggagatggcc ccggcgccta aagctcctgt
     1321 gatcttgaga aacggccctt ccaggtggaa acccagtact tccgttcctt cagtttctgt
     1381 tttggacaac tccgagagaa actcagcatc ttctccttta ggcctgggag ccagtgtggg
     1441 caaaaatccg gtaactccgt attgaggtaa aattttgcag atttctttca agtcttccgc
     1501 accgttgtcc accagcatgc gatgaatgcc gtgtatatgc aaatcaatga atccgggcgt
     1561 gatatacgat tcacccaaat caatcacatc acatcccgga ggagattctc ctatatcgat
     1621 aatttttcct ccacttgttg tgagatatcc tttaactagt tcagaatctg aaattataat
     1681 ccccttgaaa gcaatttgat tattcctttg catatgttca accttattat ttccgccgat
     1741 ggggtatggg ggtgctacgc atgcattccg ccatccatca aaatgttttc tccatttata
     1801 taagcaccgg catccgatgc caacagcacc atcaggcctt ttatgtcgtc gttgtttgcc
     1861 attcgtccca aagg
//
LOCUS       DAOSDR010000474         1839 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrimonas sp. isolate AMR_MDS_1826
            NODE_21585_length_1839_cov_3.312780, whole genome shotgun sequence.
ACCESSION   DAOSDR010000474 DAOSDR010000000
VERSION     DAOSDR010000474.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863355
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrimonas sp. (wastewater metagenome)
  ORGANISM  Petrimonas sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Dysgonomonadaceae; Petrimonas.
REFERENCE   1  (bases 1 to 1839)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1839)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:10:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,720
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 35
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1839
                     /organism="Petrimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21585_length_1839_cov_3.312780"
                     /isolate="AMR_MDS_1826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2023866"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            91..>1839
                     /locus_tag="PLI30_13425"
     CDS             91..>1839
                     /locus_tag="PLI30_13425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005833364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOI80593.1"
                     /translation="MIKIISVFFACIITLSIYAGDSGSIFIEAESFQNKGGWVVDQQF
                     MDLIGSSYLMAHGLGTPVSDATTTVNFAKTGEYTVFVRTYNWTSPWTTKEGPGKFQLT
                     VNGRVLNNKILGTKSTAWEWQEVGKVKISSGTVTIGLRDLTGFNGRCDAVYFTMDNVL
                     PPNEREALTAFRKEKLGLPEVVPNAGKFDLVVVGGGVAGTAAALTAARQGLKVALIQD
                     RPVLGGNNSSEVRVHLGGRINLDPYPKLGDVVNEIAPLRGGNAQPGDYYEDEKKLEMV
                     NAENNISLFLNFRAFKVEKGGTTIRTVFAKHIETAEELSFSAPLFADCTGDGTIGYLA
                     GADFAMGRESKAEFNEATAPEEADKMTMGSSVQWYSKEGDKSSDFPEFDYGMDFNETN
                     AQKVTMGEWTWETGMNFDQISDFERVRDYGMLVVYSNWSYLKNKSSFKEEYKNKYLDW
                     VAYIAGKRESRRLIGDMVLTEQDITDFVIYPDASAPTSWTIDLHYPDPKNTEHFPGAE
                     FKSIAVHKTIHPYPIPYRCFYSRNINNLFMAGRNISVTHVALGTVRVMRTTGMMGEAV
                     GMAASVAAKNKTTPRGV"
BASE COUNT          554 a          387 c          463 g          435 t
ORIGIN      
        1 tactgtccct gtgccgggaa gtgccaaata aaagaataaa tacgttataa tcatgacgat
       61 agactggtcg actaaacatc aattcgaaaa atgataaaaa tcatctctgt cttttttgcc
      121 tgtattatta ccttgtctat ctatgccgga gattctggtt cgattttcat tgaagcggag
      181 agttttcaaa acaagggagg atgggtggta gatcaacaat tcatggacct gataggatca
      241 tcctacctga tggcacatgg cctgggtact cccgtgagtg acgcaaccac aacggtgaac
      301 ttcgccaaga ccggagaata taccgtattt gtcagaacgt acaactggac atctccttgg
      361 accactaaag aaggccccgg caagtttcaa ttgacggtaa acggaagggt cttaaacaac
      421 aaaattttag gcacgaaaag cactgcctgg gagtggcagg aggtcggtaa agtaaagata
      481 agctccggga cagttaccat tggattgcgt gatctgacgg gttttaacgg ccgttgcgat
      541 gctgtatact ttacgatgga caacgtttta cctcctaatg aacgggaagc gttaacagcc
      601 tttagaaaag agaaactggg gttaccggaa gttgtaccca atgccggaaa attcgatctt
      661 gtggttgtcg gaggaggagt agccggcact gccgctgcgc tcaccgctgc ccggcagggc
      721 cttaaggttg ccctcatcca ggatcggccg gttttgggag ggaacaacag ttcagaagtc
      781 agggtacacc ttgggggcag gatcaacctc gatccctacc ccaaacttgg agatgtggtc
      841 aatgaaattg cccctctcag gggaggcaac gcccagccag gagattacta cgaagatgag
      901 aaaaaactgg agatggtcaa cgcagagaat aacatcagct tgttccttaa tttcagagca
      961 tttaaagttg aaaaaggggg aacaactatt cggacagtat ttgccaagca tattgaaaca
     1021 gccgaagaat taagcttttc cgcaccactt tttgcggatt gtaccggaga cgggacgatt
     1081 ggctacttag caggtgctga ttttgccatg ggaagagaaa gtaaagcaga atttaatgaa
     1141 gctaccgctc ccgaggaagc ggataaaatg accatgggct catctgtcca gtggtactca
     1201 aaagaaggag acaaaagctc cgattttccg gagtttgact acgggatgga ctttaatgaa
     1261 acaaacgctc aaaaagtaac catgggtgaa tggacctggg agacgggcat gaactttgat
     1321 caaatatccg atttcgagag agtaagggac tacggtatgc tggtagtata ctcgaactgg
     1381 tcctacctga aaaacaaaag ttcattcaaa gaagagtaca agaacaagta cctggattgg
     1441 gtagcttata ttgcaggaaa aagggaatca cgcaggctaa ttggcgatat ggtgttgaca
     1501 gaacaagaca ttaccgattt cgtaatctat cctgacgcct ctgcaccaac aagctggaca
     1561 attgatctcc attatcccga tccaaaaaac acggagcatt ttcccggggc tgagtttaaa
     1621 tcgattgcgg ttcacaaaac catacatcct tatcctatac cttaccgttg tttttattcc
     1681 cgaaacataa ataacctttt tatggcggga agaaatatca gcgttacaca cgtggcgtta
     1741 ggtaccgtga gggttatgcg tacaaccggt atgatgggcg aagcggtggg aatggctgcc
     1801 tcggtcgcag caaaaaataa aacaacacca cgcggtgtt
//
LOCUS       DAOSDR010000475         1825 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrimonas sp. isolate AMR_MDS_1826
            NODE_21797_length_1825_cov_3.664407, whole genome shotgun sequence.
ACCESSION   DAOSDR010000475 DAOSDR010000000
VERSION     DAOSDR010000475.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863355
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrimonas sp. (wastewater metagenome)
  ORGANISM  Petrimonas sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Dysgonomonadaceae; Petrimonas.
REFERENCE   1  (bases 1 to 1825)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1825)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:10:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,720
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 35
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1825
                     /organism="Petrimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21797_length_1825_cov_3.664407"
                     /isolate="AMR_MDS_1826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2023866"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            333..1499
                     /locus_tag="PLI30_13430"
     CDS             333..1499
                     /locus_tag="PLI30_13430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008662237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2961 domain-containing protein"
                     /protein_id="HOI80594.1"
                     /translation="MNLKSIYITFLLFVFTAGIFAQNREFNGLDMNMGNLYKLSNAET
                     RSISPENFTGEKGQGGMATPDPNALRNTANATHAARDLGQGWKVNPYVRINPGETFTM
                     AEIEGPGAIQHIWMTPTGNWRFSIIRIYWDDETEPSVECPVGHFFGMGWNEYTHLNSM
                     PVTVNPGSAFNSYWVMPFRKKCKITMTNINDADPMTLYYQIDYTLTDVPADAAYFHAQ
                     FRRTRINETSDYTIVDGIRGEGHYVGVYMAWQVNNNGWWGEGEIKFFMDGDNKFPTII
                     GTGTEDYFCGSYNFDRQGKYTTFTTPYAGLVQVLPPDIIYRSGQRFGLYRWHIMDPIR
                     FKKDLRITIQDLGWRHGGRYLPQQSDISSVCFWYQAEPHAKFPQLSGWQQLEMN"
BASE COUNT          549 a          368 c          420 g          488 t
ORIGIN      
        1 gccgtggata aaaccttata tatcgattcc cggattggtg atttcaagtg ttttatcagt
       61 gcccgatcgg gatttggaac agttgaattt aaagctggca ggccgatctt gaatgtggtg
      121 tatggaacga tcgaagtaga aaagtacaat attagcggta acatgcttga tttataatag
      181 gattcattaa tcatttaatt cagggtaatc acacgttaaa tgacgaagaa attttatatt
      241 tgcccgatat taataattca aatctgctat acattgcaaa aacaggtgtt tatcccatta
      301 aaatattgta gaaccaaata aaataagcaa ctatgaactt aaaatcaatt tacattactt
      361 ttttattatt tgttttcacg gcaggtattt ttgctcaaaa ccgtgagttc aacggcttgg
      421 atatgaacat gggtaacttg tacaagcttt caaatgccga aacccgttcc atcagtcccg
      481 aaaactttac cggtgaaaag ggacagggag gaatggccac tcccgacccg aatgctctcc
      541 gcaacacggc gaacgctact catgcagcac gcgatttggg acagggatgg aaagtaaatc
      601 cctacgtccg catcaatcca ggagaaacgt ttaccatggc tgaaattgaa gggcccggcg
      661 ccattcaaca catttggatg acacctacag gcaactggcg cttttccatc atccgcatat
      721 actgggatga cgaaactgaa ccttcggtgg aatgcccggt cggccatttt ttcggtatgg
      781 gatggaacga atatacgcac ctgaactcga tgcctgttac ggtaaatcca gggagtgcgt
      841 ttaattctta ctgggtgatg cctttcagga aaaaatgcaa aataaccatg accaatatca
      901 acgatgccga tccaatgaca ctttattatc agatcgacta cacattgacc gatgttccgg
      961 cagacgctgc ctattttcat gcgcagttcc gacgtacaag aataaatgag acttccgatt
     1021 acaccattgt tgacggtatc cggggagaag gacattacgt aggcgtgtac atggcatggc
     1081 aggtaaacaa caacggttgg tggggcgaag gagagattaa gttttttatg gacggagaca
     1141 ataaattccc gaccatcata gggactggga ctgaagatta tttttgcggt tcctacaatt
     1201 ttgaccgtca aggtaaatac actacgttta ccacacctta tgccggattg gttcaggtgt
     1261 tgccccctga tattatttat aggtcgggac aacgtttcgg attatatcga tggcacatca
     1321 tggatcccat ccggtttaaa aaagacctgc ggataactat tcaggatttg ggctggaggc
     1381 atggtggacg ctatttgcca cagcaatcgg atatctcgtc tgtatgtttc tggtaccagg
     1441 ccgagcctca tgccaaattc ccgcagttgt ccggttggca gcagttagaa atgaattaag
     1501 agaacaaatc gcaatgaaag gagcgtggtc gtaccagaga ggatttctgt ttcgctcact
     1561 ggcgcttatt gtcgccgaca aaggctaggt tctcgcaccc attcccgatt taaacattat
     1621 gcaatgttac ggatcacttc ccggagatta gtaaaatcag gtaaaagaga attgttaaga
     1681 atgaaaaaca accataacaa aaacatgata aaacgaaatc aatttggtgg agtcgttgct
     1741 gtagtggtga ttggggggtt gattgcactc tcctgcacgg gtaacgctaa aaaatcatcg
     1801 gataatatga aaaaggagta tgaaa
//
LOCUS       DAOSDR010000476         1821 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrimonas sp. isolate AMR_MDS_1826
            NODE_21856_length_1821_cov_4.328992, whole genome shotgun sequence.
ACCESSION   DAOSDR010000476 DAOSDR010000000
VERSION     DAOSDR010000476.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863355
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrimonas sp. (wastewater metagenome)
  ORGANISM  Petrimonas sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Dysgonomonadaceae; Petrimonas.
REFERENCE   1  (bases 1 to 1821)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1821)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:10:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,720
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 35
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1821
                     /organism="Petrimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21856_length_1821_cov_4.328992"
                     /isolate="AMR_MDS_1826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2023866"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..738)
                     /locus_tag="PLI30_13435"
     CDS             complement(<1..738)
                     /locus_tag="PLI30_13435"
                     /inference="COORDINATES: protein motif:HMM:NF025104.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate glucose
                     phosphotransferase"
                     /protein_id="HOI80595.1"
                     /translation="MNLAVFIVFQFSPVHDYMDVPRRNLYYLHANISEIIAYTLYGKR
                     NFFFTDKYRYRLRIISIRFLVLIITLFALGEIFLPKGYHRVFLLEYVAFFFLFKVVVF
                     YFIYQLHRYRYAKGYSFNRVVILGTNNSSRVVGKILNNNPTLGFKLVGYISDKGNGAG
                     EDLLGRLTELPALAKDHKINMIFVTNPKYFTLKNTKELLALCNKTGLRVRYILMNGYW
                     NKCMYRKVESARFFEMFNPQQIPLDDLT"
     gene            complement(881..1636)
                     /locus_tag="PLI30_13440"
     CDS             complement(881..1636)
                     /locus_tag="PLI30_13440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WecB/TagA/CpsF family glycosyltransferase"
                     /protein_id="HOI80596.1"
                     /translation="MLHEFNLFNGSLQEINPSKKLITTLNAHSFNTLHKDIYFREALK
                     SSDMLLPDGVSIVWALRLLIGEKLKKIAGADLFRYEMDRIHSTKGKCFFLGSSEKTLN
                     LIRERAAKEYPYVEVYSYSPPYKPEFSDEESQRMVDAVNEIEPDVLFIGMTAPKQEKW
                     AFKYYPQLKAGHICCIGAVFDFYAGTVKRAPGWMISIGMEWFYRLVKEPKRMWRRYLI
                     GNTLFIKHILKEKLVALYHYKNTRPRVVFRDLL"
     gene            complement(1642..>1821)
                     /locus_tag="PLI30_13445"
     CDS             complement(1642..>1821)
                     /locus_tag="PLI30_13445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI80597.1"
                     /translation="EHYKKMDFDYIDLGISSMCGDPNVGLIRFKEIHNCETSLRFTFW
                     WSPDNNQSFQINPKK"
BASE COUNT          481 a          400 c          406 g          534 t
ORIGIN      
        1 cgtcaggtcg tccagaggga tctgctgggg gttgaacatc tcgaagaaac gggcggactc
       61 tacctttcgg tacatgcact tgttccagta cccgttcatg aggatatacc gcactctcag
      121 tccggtttta ttgcacaggg caagaagctc tttcgtgttt ttcagggtaa agtacttggg
      181 gttggtcaca aagatcatgt ttattttgtg atccttcgct aaagcgggta actccgtcaa
      241 tcttcccagc aggtcctctc cggcaccgtt ccctttgtca gaaatataac ccaccagctt
      301 gaatcccagc gtagggttgt tgttgagtat ttttccaact acccgactcg agttgtttgt
      361 acccaatata accaccctgt tgaatgaata tcccttcgcg tagcgatatc gatggagctg
      421 ataaatgaaa taaaacacta ctaccttgaa caggaaaaag aatgccacat actccaataa
      481 aaacacccgg tgataccctt tgggaaggaa gatctctccc agcgcaaaaa gggtgatgat
      541 caaaacaagg aaacgtatgc tgataattct caaccggtac ctgtacttgt cggtgaagaa
      601 aaaatttctt ttgccgtaca aggtataggc aatgatttcc gaaatattcg cgtgtaaata
      661 atacaggttc cttcggggta cgtccatata atcgtgaacc gggctaaact gaaaaacaat
      721 aaagaccgct aagttcaaaa gaaacaagtc tattaaaaga taaaagaacc ttattcccgg
      781 gtgatctgtt tccataactg cgaaataata aaatagaata cgttgtacat gctttgttgc
      841 gtttatgctg aaaaacaagg atgccttgtc ttttagcctt ttagagtaaa tcgcgaaata
      901 ctacacgggg ccgagtgttt ttataatgat agagtgccac cagtttttcc ttcaggatat
      961 gcttgatgaa taatgtattc ccgatgaggt acctgcgcca catgcgctta ggttctttga
     1021 ccaatcggta aaaccattcc ataccgatgg atatcatcca gccgggtgct cttttcacgg
     1081 ttccggcgta gaagtcgaat acggcgccga tgcaacagat gtggcccgct tttaactgag
     1141 ggtaatactt gaaggcccat ttttcttgct tgggcgccgt catgccgata aagaggacgt
     1201 cgggttcgat ttcattgact gcatctacca tcctttggct ctcttcatcg ctgaattccg
     1261 gtttgtaagg cggtgagtag ctgtagacct ctacgtaggg atactccttt gctgctctct
     1321 cccggatgag gttcaacgtc ttttccgaac ttcccagaaa gaaacatttc cctttcgttg
     1381 agtggatccg atccatttcg taccggaaca gatccgctcc ggcaatcttt tttaattttt
     1441 cgccgattaa caggcgaagc gcccagacga tacttactcc gtcgggtagc agcatgtcgc
     1501 tcgacttcag ggcttcccgg aaataaatgt ctttatgtag tgtattaaac gagtgggcat
     1561 tgagtgtggt gatcagtttt tttgacggat tgatctcttg taaagacccg ttgaacaagt
     1621 taaattcgtg taacatattg cttatttttt agggttaatt tgaaatgatt gattgttgtc
     1681 agggctccac caaaaggtga acctgagtga tgtctcacag ttgtggattt ctttaaaccg
     1741 gattaatccg acgttaggat ctccgcacat gctggatatt cccagatcga tgtaatcgaa
     1801 gtccatcttt ttatagtgtt c
//
LOCUS       DAOSDR010000477         1813 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrimonas sp. isolate AMR_MDS_1826
            NODE_21991_length_1813_cov_1.954494, whole genome shotgun sequence.
ACCESSION   DAOSDR010000477 DAOSDR010000000
VERSION     DAOSDR010000477.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863355
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrimonas sp. (wastewater metagenome)
  ORGANISM  Petrimonas sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Dysgonomonadaceae; Petrimonas.
REFERENCE   1  (bases 1 to 1813)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1813)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:10:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,720
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 35
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1813
                     /organism="Petrimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21991_length_1813_cov_1.954494"
                     /isolate="AMR_MDS_1826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2023866"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1247)
                     /locus_tag="PLI30_13450"
     CDS             complement(<1..1247)
                     /locus_tag="PLI30_13450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI80598.1"
                     /translation="MNCKLVAPVIFVILLTVGCIRNDIPGVEQLVSSGGRTFIVTATI
                     PDASAKTRTSSSFGLETKDIIVKWKQGDKINLFFKQDYRIKEQRDVTLYDFSNGNKTA
                     RFDITVPQDIDITQPYTLYGTHGAESKIAGDKIMASGMTRAGVFDSFDIPLWFKTEVP
                     ASGIVPPVSFQHLGALLVTQLENTSDENLNIFIIPLVYRKTDYALVGFREPEVSPVSL
                     REEIPGFNLITQTVEDYAPFVSSQPFTTIVDARETEAFAYWGIPRADNIPETVLGITV
                     PKPGGQDATFFSTTSKPARDALQTGHAYHISAEWDGTELRFPGEVEPNRPRLPIEYVA
                     EYNVSVTAGTFTDSHANNKSGYFDWDAARTACPAGYHLPSQDEWRGILPNHLYGVYVN
                     FQGIGKGVDNCVEEIVVGGQEKRT"
     gene            1566..>1813
                     /locus_tag="PLI30_13455"
     CDS             1566..>1813
                     /locus_tag="PLI30_13455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI80599.1"
                     /translation="MKELLSAYYPITVVYTTVFLTLGTVLLFGKVPRLERLQNYLSGK
                     RYFTASFLFLGIFGVIELLTAPLTRMSGTHYTYNDIFG"
BASE COUNT          441 a          439 c          451 g          482 t
ORIGIN      
        1 gtacgctttt cctgtccacc tacgactatc tcctccacgc aattgtcaac tcctttccct
       61 ataccttgga agtttacgta aactccataa agatgattag gcagaatgcc ccgccactcg
      121 tcctgcgaag gcaggtgata gcccgctggg caagctgttc tggcggcatc ccagtcgaaa
      181 tagccgctct tgttgttggc atggctgtcg gtaaacgtgc cggcagtgac gctcacgttg
      241 tattccgcca cgtactctat cggaagccgg ggacgattag gttccacctc accgggaaac
      301 cgcagttccg tgccgtccca ttcggcggag atgtggtagg catgaccggt ttgaagggca
      361 tcacgggcgg gcttggatgt ggtggaaaag aaagtggcgt cctgcccgcc cggtttcggg
      421 acggttatcc ccagcaccgt ttcgggaatg ttgtccgcac gggggattcc ccaataggca
      481 aacgcttctg tttcccgcgc atcaacaatt gtggtaaatg gttgggaact cacaaatggg
      541 gcataatctt ccaccgtttg ggtaatgagg ttgaaaccag ggatttcttc ccgtaggctg
      601 acagggctta cttcaggttc tcggaatccc acgagcgcgt agtccgtttt ccgatatact
      661 agcgggatga taaatatgtt caggttttcg tccgaggtat tttcgagctg ggtaaccagc
      721 aacgcgccca agtgttgaaa agaaacgggc ggaacaatgc cgcttgccgg aacctccgtt
      781 ttaaaccaca gggggatatc gaagctgtcg aacacgcccg cccgcgtcat gccggacgcc
      841 attatcttat cgccagcaat cttcgattcg gcgccgtgcg taccgtagag ggtgtagggc
      901 tgagtgatgt cgatgtcctg cggtacggta atgtcgaacc tggccgtttt gttgccgtta
      961 gaaaaatcgt agagcgtcac atcgcgttgc tctttgatgc ggtagtcctg cttgaaaaag
     1021 agattgattt tgtcgccctg tttccatttt acgatgatgt ctttagtctc caggccgaaa
     1081 ctggaagaag tacgggtttt cgcactggca tcgggtatgg tagcggttac gataaaagta
     1141 cgtcccccac tggaaacgag ttgttcgacg ccgggaatgt cgttgcgtat gcatcctact
     1201 gtcaacagga tgacgaaaat gacgggggca actaatttgc aattcataac ggataattta
     1261 cttataatta aaaacttaaa atttacgata aacatgcaac tgaaaacgag aatgcaggct
     1321 tctttgaacg gcgttttaga accggctgta tttatcggct taaatttcac gcctaccggt
     1381 gtatgtttcg catttgacat gtacatcaaa cccggcggca aatataaaac ctaagatttg
     1441 cacctgcgac aattgtcgta aaggcaacaa aaaaagttta attttaacat ttttaacaaa
     1501 ctattcatcg ccgggtaacc tcgaactccc gttttctaac tttataaaaa cactatcttt
     1561 gcaccatgaa agagctactt tccgcatatt acccgataac ggttgtttac accaccgttt
     1621 tccttacgtt gggaacagtg ttgctgttcg gaaaggttcc aaggctggag cggttgcaaa
     1681 actatctttc gggaaagcgc tattttacag catcgtttct ctttctgggt attttcggcg
     1741 tgatagaatt gctgacagcg ccattgacac gcatgtccgg cacacactac acttacaacg
     1801 acatctttgg aat
//
LOCUS       DAOSDR010000478         1795 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrimonas sp. isolate AMR_MDS_1826
            NODE_22253_length_1795_cov_4.029885, whole genome shotgun sequence.
ACCESSION   DAOSDR010000478 DAOSDR010000000
VERSION     DAOSDR010000478.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863355
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrimonas sp. (wastewater metagenome)
  ORGANISM  Petrimonas sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Dysgonomonadaceae; Petrimonas.
REFERENCE   1  (bases 1 to 1795)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1795)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:10:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,720
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 35
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1795
                     /organism="Petrimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22253_length_1795_cov_4.029885"
                     /isolate="AMR_MDS_1826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2023866"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..481
                     /locus_tag="PLI30_13460"
     CDS             <1..481
                     /locus_tag="PLI30_13460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI80600.1"
                     /translation="SWRCCRSRQYRSWVANTVVFTYSQFTGDCYVLFFHPACPDPLSV
                     ADLQDNAGSLEKNKEQTFHHHNACSLSSDPYLGRRDRLWDFSLQMGHLVRNQNLQDRG
                     SEGAEGMYYEGAYSAISEGIAVETRFNTAMSMTCVWGELKVWRDSTHLPDQYLPIGG"
     gene            485..1585
                     /locus_tag="PLI30_13465"
     CDS             485..1585
                     /locus_tag="PLI30_13465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI80601.1"
                     /translation="MKKIGIFCLAFLLLISVFCIVGFKKAEAQDKGKDPKRGYDIVSP
                     TLSPDNKKIMFVVCHPDICDLAVYEIITGRMVRFHPSSDKSNGVPSYSSDGKKITFIS
                     GDFGDNYNVFVMNIDGSGLRQLTHDYNNGKMKDGPDIVVKLNAGPSFSSDGERIIYKK
                     SGIKRQFYRPGRQILHWDIYEMDIKTGKERRLTNYRFFHIAGRPAYMPDGKRFIFSGE
                     GAKNHTGIGPKDFEAYTKLYKQNNIFIMDGVKNALEPAFTYGDWTQEPSVAKDGTILF
                     MARTCIQKGKKPVCTYDLFSRRGNETKAVTNKRFSRMAQPRISFDGTHAVFLASRWEG
                     AGPSLFVTKIDSSESRYFELPPWEAIVQLKHP"
BASE COUNT          501 a          381 c          440 g          473 t
ORIGIN      
        1 ctcttggcgg tgttgtcgca gcaggcagta tcgttcgtgg gtggcgaata cagtcgtctt
       61 cacatactct caattcaccg gagactgcta tgtactattt tttcatcctg cctgtcctga
      121 tcctctatcc gttgctgacc tacaagataa cgcgggaagt ctggaaaaaa acaaagagca
      181 aacgtttcac catcataacg cttgcagtct ttcttctgat ccctacctgg gacgtcgtga
      241 tcggctatgg gatttttctc tacaaatggg ccacctggtc cggaaccaaa atctacaaga
      301 ccgtggaagc gaaggggcgg agggaatgta ttacgaagga gcatactcgg caatatcgga
      361 gggcattgca gttgaaaccc ggtttaacac ggcaatgtct atgacctgtg tgtggggtga
      421 attaaaggtg tggcgtgata gcactcacct tcctgatcag tatttgccga ttggaggata
      481 gtaaatgaaa aaaatcggta ttttttgtct agcatttcta ctgcttattt cggtgttctg
      541 catagtgggc ttcaaaaaag ccgaagccca agacaaaggc aaagacccca aaagaggtta
      601 tgatattgta tccccaaccc tctcaccgga taataaaaag attatgtttg ttgtctgtca
      661 tccagatatt tgtgaccttg ccgtctatga aatcatcacg ggtaggatgg tacgatttca
      721 tccatcctct gataaatcta atggagtgcc ttcctattca tcggatggca aaaaaatcac
      781 attcatatcc ggcgactttg gtgacaatta taatgtattt gtcatgaaca ttgacggcag
      841 tggtcttagg cagttgactc atgattataa caacggcaag atgaaggatg gccctgatat
      901 tgtggttaaa ctcaacgcgg ggccttcatt ttcttccgat ggtgaacgca tcatctataa
      961 aaaatcagga attaaaaggc agttttaccg accaggcaga cagatcctgc attgggatat
     1021 ttatgagatg gatattaaaa ccggaaagga gcgcaggctg acgaattatc gcttttttca
     1081 tatcgccggg cggcctgctt atatgccgga tgggaagcgg ttcatttttt ctggcgaagg
     1141 cgccaagaac catacgggta tagggccgaa ggattttgaa gcgtacacga aactatacaa
     1201 acaaaataat atcttcatca tggatggagt caagaatgcc ctggagcccg ccttcacgta
     1261 tggtgactgg acccaagagc cttctgttgc caaagacggt acgattcttt tcatggcacg
     1321 gacatgcatc cagaaaggca agaaacctgt ttgcacctac gacctctttt cccggagagg
     1381 aaacgaaacc aaagcggtca cgaacaagcg tttttctcga atggcgcaac ctcgaatatc
     1441 tttcgatggg actcatgctg tgtttttggc cagcagatgg gagggggcgg gaccttccct
     1501 gtttgtgacc aagattgata gttcggaatc gaggtatttc gagctgccgc cctgggaagc
     1561 gatagtacaa ttgaaacatc cgtaaacgaa cgagccaaac gaataaagga gggttggcca
     1621 tggatatgag agcttttgta gctaaatatg gactatacgg tgaatatggc ggccctgatc
     1681 attcggggga acaaaggaat gcggatggat caatctatag agatgaaaat aataataccg
     1741 tcccctatta ttatgatccc atcgacctgt tggattacgc tttcaagtta catga
//
LOCUS       DAOSDR010000479         1795 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrimonas sp. isolate AMR_MDS_1826
            NODE_22267_length_1795_cov_1.802299, whole genome shotgun sequence.
ACCESSION   DAOSDR010000479 DAOSDR010000000
VERSION     DAOSDR010000479.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863355
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrimonas sp. (wastewater metagenome)
  ORGANISM  Petrimonas sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Dysgonomonadaceae; Petrimonas.
REFERENCE   1  (bases 1 to 1795)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1795)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:10:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,720
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 35
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1795
                     /organism="Petrimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22267_length_1795_cov_1.802299"
                     /isolate="AMR_MDS_1826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2023866"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1499)
                     /locus_tag="PLI30_13470"
     CDS             complement(<1..1499)
                     /locus_tag="PLI30_13470"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HOI80602.1"
                     /translation="MKNILTFLIILLTFNGLLSQTINVLDRATLQPVPGAVIMVKEGS
                     LEQTSTTDQSGKVSTKALGDEAVIIVRALGYHTFQTKFNELKKSNYRILLTGKVYDLD
                     ETVISASRFEEKREDVPQQVLVMKRRDLELLSQPTTAELLQKSGEVLVQKSQLGGGSP
                     ILRGLEANKVLLVVDGIRMNNAIYRGGHLQSIITLDNSALEKAEVVYGPGSVIYGSDA
                     LGGVMHFHTLKPRTAGPDKSFYSRTQAFTRYSSAYNEKTAHADVNLGWKNWASLTSVT
                     VSDFGDLRQGDVRNPFYGDWGKCLFYVERINNKDSALINSNPNIQKRSGYSQYDFMQK
                     FYLKASEKAEHTLNFQYSTSSDINRYDRLTETSGGLPRYAEWYYGPQKRLLAAYHLDL
                     HNSRIYDQARITLAWQNLEESRHNRSFGKSKLSHRTEKVDVLSLNADFQKQVGRHELR
                     YGVEGTYNDVNSSAYWENIKDGSTGSLDTRYPDGGSTMRTLAVYFTHAW"
     gene            complement(1613..>1795)
                     /locus_tag="PLI30_13475"
     CDS             complement(1613..>1795)
                     /locus_tag="PLI30_13475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI80603.1"
                     /translation="ATGGRMGLINPAMQLSLYFIPAAIRITPLPAKYLVFSGYRICPP
                     LPFEPGVPSPPPWCTS"
BASE COUNT          493 a          417 c          375 g          510 t
ORIGIN      
        1 tcccacgcat gggtaaaata cacagccaga gtacgcatgg tagaaccacc atccggataa
       61 cgtgtgtcga gagatcctgt gctgccatct ttgatatttt cccaatatgc cgagctgttt
      121 acatcgttgt aggttccctc taccccataa cgcaattcat gccttccgac ttgtttctga
      181 aaatcagcat tcaaagaaag cacatccact ttttccgttc tgtggctgag tttgcttttg
      241 ccaaaactcc tgttgtggcg gctctcttct aaattttgcc atgcaagtgt aatccgtgcc
      301 tggtcgtaaa tgcggctgtt gtgcaggtca aggtgataag cggccagaag ccttttttgt
      361 ggaccatagt accattcggc ataccggggc aatccaccac ttgtttctgt caatctatcg
      421 taacggttga tatccgacga ggttgaatat tgaaaattaa gggtatgctc tgccttctct
      481 gacgctttta aataaaactt ttgcataaaa tcgtactggg agtatccaga gcgtttctgg
      541 atgttggggt tggaattaat caaggcactg tctttattgt tgatgcgttc aacataaaac
      601 aagcatttgc cccaatcacc ataaaaggga ttacgcacat ccccttgcct taagtcacca
      661 aaatcggaaa ccgttaccga agtcaaagag gcccaatttt tccatccaag attcacatca
      721 gcatgtgcgg ttttctcgtt ataggctgaa gaataacgcg taaatgcctg ggtgcggctg
      781 taaaatgact tatccgggcc tgccgtgcga ggctttaatg tgtgaaaatg catcacaccg
      841 cccaatgcat cgctcccata aatcaccgaa cccggaccat agaccacttc agctttttca
      901 agcgctgaat tatcgagggt gatgatgctc tgcagatgcc ctccgcggta gatggcgtta
      961 ttcattcgaa taccgtccac aacgagcaac accttattgg cttccaggcc tcttaaaata
     1021 ggactgcctc ctcccaattg gcttttctgc accaatactt caccgctttt ttgtaatagt
     1081 tcggctgttg tgggttgact cagtaattca aggtcgcggc gtttcattac caacacttgc
     1141 tgaggtacgt cctcccgctt ttcttcaaaa cgactggccg aaataaccgt ctcatccaga
     1201 tcatacacct tcccggtcag taaaatccta taattgctct tcttcagttc gttaaatttg
     1261 gtttggaatg tgtgatatcc caatgcacga actataatca ccgcttcatc acccaaggcc
     1321 ttggttgaca ccttcccgct ctggtcggta gttgaagttt gttccaaact tccttctttt
     1381 accattataa ccgcacctgg aacaggttgt agagtcgcac gatcgagtac attgatggtt
     1441 tgggatagta atccattgaa agtcaataat attattaaaa aagttaaaat gtttttcata
     1501 gcaattttta atttcgttaa aaaagaataa taacatagag cgacatttat cgtaatgtca
     1561 ctacctcacc cttaaaaagg aggaggtttt aaataataca agagtataaa atttatgagg
     1621 tacaccaagg aggaggagaa gggactcccg gctcaaacgg caagggtgga caaatcctat
     1681 agccactgaa aacaaggtat tttgcgggta aaggagtgat acgaatagca gcaggaataa
     1741 aatacaaaga aagctgcatg gctggattta tcaggcccat tcttccaccg gtagc
//
LOCUS       DAOSDR010000480         1788 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrimonas sp. isolate AMR_MDS_1826
            NODE_22360_length_1788_cov_4.088863, whole genome shotgun sequence.
ACCESSION   DAOSDR010000480 DAOSDR010000000
VERSION     DAOSDR010000480.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863355
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrimonas sp. (wastewater metagenome)
  ORGANISM  Petrimonas sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Dysgonomonadaceae; Petrimonas.
REFERENCE   1  (bases 1 to 1788)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1788)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:10:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,720
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 35
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1788
                     /organism="Petrimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22360_length_1788_cov_4.088863"
                     /isolate="AMR_MDS_1826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2023866"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..798
                     /locus_tag="PLI30_13480"
     CDS             <1..798
                     /locus_tag="PLI30_13480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006841661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HOI80604.1"
                     /translation="ENEEIIRENVDNYKKRFGEDIPVLYHPLIRSEEACFRSSAQAAE
                     LADKYGSRLHVLHLSTAREMSLFSKSSVKDKKITAEVCVHHLWFTDEDYARLGTRIKW
                     NPAIKTRADRDALRSALISGKLDIVATDHAPHLLSEKEGGCLKASSGGPLVQHSLQAM
                     LELSLQGLWSKEFVVEKMCHAPAELFQVRGRGYIREGYFADLVLVNPDKPYTVGKNNI
                     LYKCGWSPFEGETFRHSIEKTFVNGNLVFDKGNVVESAPGEALTFNR"
     gene            870..>1788
                     /locus_tag="PLI30_13485"
     CDS             870..>1788
                     /locus_tag="PLI30_13485"
                     /inference="COORDINATES: protein motif:HMM:NF024918.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family protein"
                     /protein_id="HOI80605.1"
                     /translation="MKFLFTVQGEGRGHFTQSLALASMLRKHGHEVVAVLVGKDDSRQ
                     IPRFYLDKINAPVFDFRSPNFTALYKQKRPNLVLSVIGNFSQSFIFRKSILFVKSKIE
                     EYRPDAVVNFYEMVTGVAFRTYRFDKKLNVQLISIAHQYMLLNPKYKTTSEQDIKYYF
                     LRMITKVTCQCSSKILALSFRDMPGSIEKNLVVVPPLLRQEVFENEPSDGDYIHGYMI
                     NTGYYEEVLDWHTKNPHIPLRFFWDKKDADEVTVIDENLILYRIDDDLFLKSMAGSMA
                     YSTTSGFESVCEALYYKKPILMIPVHVEQE"
BASE COUNT          492 a          381 c          445 g          470 t
ORIGIN      
        1 gagaacgaag agattatacg tgaaaatgta gataactata agaaacggtt cggagaagat
       61 attcccgttc tgtaccatcc gcttatccgc agtgaagaag catgcttccg ctcttcggca
      121 caagctgcgg aactcgccga caagtacggt tcccgcctgc atgtgttgca tctttccact
      181 gcccgggaaa tgagtttgtt cagtaagtcg tctgtaaaag acaaaaaaat aacagcagaa
      241 gtatgcgttc atcacctttg gtttaccgat gaggattatg cccggttggg cacacgcatc
      301 aaatggaatc cggcgataaa aacgcgtgca gaccgtgatg ccttgcgttc tgcgctgata
      361 tcgggaaagc tggatattgt ggcaactgat catgctccgc atttgttgag cgagaaagag
      421 ggcggttgcc tgaaagcttc ctctggcggg ccgttggttc aacactccct gcaggctatg
      481 ttggagttgt cgttacaagg gctctggtcg aaagaatttg ttgtggaaaa aatgtgccat
      541 gcgcctgcag aactttttca ggtgagaggc cgggggtata tccgcgaagg atattttgcc
      601 gacctggtgc ttgtgaatcc tgataaacct tatacagtcg gtaaaaacaa tattctgtac
      661 aaatgtggct ggtcgccgtt cgaaggtgaa actttccggc acagcatcga gaaaacgttt
      721 gtgaacggga atctggtgtt tgataaaggt aatgtagttg aatctgctcc gggagaagca
      781 ttgacattca accggtaaat ccgtgtgagg ttatggctgt tatgtggcaa taattttata
      841 cctttgcgcg aaatcaagaa taaataactg tgaaattttt atttaccgta cagggcgagg
      901 gcaggggaca ctttacgcaa tcgctcgcat tggcgtctat gttaagaaaa cacggacacg
      961 aagtggtggc cgtattggtg ggtaaagacg actcgcgaca aattccccgg ttttatctcg
     1021 ataaaatcaa tgcgcctgtt tttgatttta gaagccctaa tttcaccgca ttatacaaac
     1081 aaaaacggcc aaacctcgta ttgagcgtca ttggtaattt ttcgcaatcg ttcatctttc
     1141 ggaaaagcat tcttttcgtc aaatccaaga tagaggaata tcgtcccgac gcggtggtca
     1201 atttttatga aatggttacc ggagtggctt ttagaaccta tcggtttgat aagaagttaa
     1261 atgttcaatt aatcagcatc gcacaccagt acatgctgct gaatccgaaa tacaaaacca
     1321 ccagcgaaca ggacatcaag tattattttc ttcggatgat aacgaaggtt acctgccagt
     1381 gttcgtccaa aatcttggca ctttcgttcc gtgatatgcc cggaagcatt gagaagaacc
     1441 tggtggttgt tccgccactg ctcagacagg aggtctttga aaatgaaccc tccgacggag
     1501 attatatcca cggctatatg atcaataccg gatattacga ggaagtgctg gactggcaca
     1561 ctaaaaaccc ccacattccg ctgcgcttct tctgggacaa gaaggatgcc gatgaggtga
     1621 cggtgataga cgaaaacctg atactgtatc gcatcgatga cgatcttttc ctgaaaagta
     1681 tggcgggcag tatggcatat tccaccacct cgggatttga gtcggtgtgc gaggcactct
     1741 attacaaaaa acccattctg atgattcccg tacacgtgga gcaggagt
//
LOCUS       DAOSDR010000481         1784 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrimonas sp. isolate AMR_MDS_1826
            NODE_22430_length_1784_cov_3.887796, whole genome shotgun sequence.
ACCESSION   DAOSDR010000481 DAOSDR010000000
VERSION     DAOSDR010000481.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863355
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrimonas sp. (wastewater metagenome)
  ORGANISM  Petrimonas sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Dysgonomonadaceae; Petrimonas.
REFERENCE   1  (bases 1 to 1784)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1784)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:10:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,720
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 35
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1784
                     /organism="Petrimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22430_length_1784_cov_3.887796"
                     /isolate="AMR_MDS_1826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2023866"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            284..1012
                     /locus_tag="PLI30_13490"
     CDS             284..1012
                     /locus_tag="PLI30_13490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019539831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase"
                     /protein_id="HOI80606.1"
                     /translation="MKKETREKMVKTLNIIAKDRKRTNILKSSEQKLIAYLVQKVPLW
                     INSDGLTAIGFFGNVMVASSIVLGAFFSRYWLLLSIVGFAINWFGDSLDGRIAYYRNN
                     PRKWYGFSLDVTVDWVGTILIGLGFTVYAEGVWKYLGFLFVVLYGWQMITAQLRYKVG
                     GQYSIDSGIFGPTEVRIILGAIITLEAFIPHSIYYLTTLATAFLLYSNFVEAKKLLKL
                     ADKRDVEEKRIKAEDLQNRQRENA"
     gene            1308..>1784
                     /locus_tag="PLI30_13495"
     CDS             1308..>1784
                     /locus_tag="PLI30_13495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019539829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase"
                     /protein_id="HOI80607.1"
                     /translation="MKKSTIWLLAVVMFVAFFSLLFLQVRYMKASMSVREQQFNEMVK
                     RSLINVSRDLEQEQTRRYLEEDMLESENRYSQYKQSGSSTNIVTEQTRATITNPDGSE
                     TRIEQIIQNQSSIDEIGRGGVFLSPRHGVNTISKTSFDLQQSFSKRYLYEQALMNEV"
BASE COUNT          589 a          340 c          364 g          491 t
ORIGIN      
        1 caactgaagt tacggaaaca actgaaaatg aatccgtaga accaaaaaca gaagctccgg
       61 ctaatgaagc tccggctgaa gagccaaaag cggaataatc agtctaccac ataccttttt
      121 aaatgcgcct gaccggcgca ttttttttgt tttgccataa attgttaact ttttttaaag
      181 ataaattatc cttaacaaat tcgaaacatc catttttaaa gctaaatttg tagattaatt
      241 acttcgtgac tatactgtat ttccacgtgt aaaaattacg gaaatgaaaa aagaaacaag
      301 agaaaaaatg gttaaaacgc taaatatcat agcgaaagac cgaaaaagaa ccaatatatt
      361 aaaaagctcg gagcaaaaat taatagcata cctggttcaa aaagttccgt tatggataaa
      421 ttcggatgga ctgaccgcca tcggtttttt tggaaatgtc atggtcgcgt ccagtattgt
      481 gttaggtgct tttttcagcc gctactggct actgctatcc atcgttggat ttgcgattaa
      541 ctggtttggc gactcactcg acggcagaat tgcctattac cggaacaatc cacgtaaatg
      601 gtacggattt tcgttggacg ttaccgtcga ttgggtaggt accatcttaa taggactcgg
      661 atttacggtt tatgcagaag gtgtctggaa atacttgggc tttctgttcg tcgttctgta
      721 cggatggcag atgatcacgg cacaactgag gtataaggta ggcggacaat attccatcga
      781 ttcgggcata ttcgggccca ccgaagtccg catcatcctg ggagcaatta ttaccttgga
      841 agcatttatt ccccattcca tatattacct cactacattg gcaacagcct ttttgctcta
      901 ttcaaatttc gtggaagcaa aaaaattact aaaacttgcc gataaacgtg atgtggaaga
      961 gaaaagaatt aaagcagagg atcttcaaaa ccggcaaaga gaaaacgcct gatcctccga
     1021 aataccagca agaccccgat atcatcctat cgggtttttt attggcaata aaattttcgg
     1081 caggcattga ctgcctcagc caacgcaaat tttccctaaa aagaaagctg cgctttaact
     1141 attctcttaa ataatgttgt aagccaacta cttttacgat tttttataat tttaagggga
     1201 tatgttaaac aaaaaagccg tttatttgtt aaattgataa ctaaaaacga tctttaatat
     1261 ttttaaattt gtcaactaaa aaaaacggag ataggtaaag caaaagtatg aaaaaatcga
     1321 ctatctggtt gcttgcagta gttatgttcg tagctttctt ctcgttgcta ttccttcagg
     1381 taagatatat gaaagcaagt atgtctgtga gggagcagca atttaacgag atggtgaaga
     1441 gaagccttat taatgtatcg agagatttgg aacaggaaca aacccggcga tacctggagg
     1501 aagatatgct ggaatcggaa aacaggtact ctcagtacaa acagtccgga tcttcaacca
     1561 acatcgttac cgaacaaacc cgggcaacca ttacgaatcc cgatggaagt gaaacaagga
     1621 ttgagcagat cattcaaaac caaagttcaa tagacgaaat aggaagaggg ggagtatttt
     1681 tatcgccacg gcacggcgtg aacacgatct caaaaacttc gtttgacttg caacaatcgt
     1741 tttcaaaacg ttacctgtac gaacaggcgc tgatgaatga ggtt
//
LOCUS       DAOSDR010000482         1781 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrimonas sp. isolate AMR_MDS_1826
            NODE_22482_length_1781_cov_3.073581, whole genome shotgun sequence.
ACCESSION   DAOSDR010000482 DAOSDR010000000
VERSION     DAOSDR010000482.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863355
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrimonas sp. (wastewater metagenome)
  ORGANISM  Petrimonas sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Dysgonomonadaceae; Petrimonas.
REFERENCE   1  (bases 1 to 1781)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1781)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:10:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,720
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 35
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1781
                     /organism="Petrimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22482_length_1781_cov_3.073581"
                     /isolate="AMR_MDS_1826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2023866"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..586)
                     /locus_tag="PLI30_13500"
     CDS             complement(<1..586)
                     /locus_tag="PLI30_13500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010799862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma-70 factor"
                     /protein_id="HOI80608.1"
                     /translation="MTNNNDYNSSVNLQAISQSQFEELFCSFYPQVAAYATVILDNKT
                     AGEDIAQEVFAYVWEKRKSLQVDVGFLSYLFQTAYSRCIDHIKKNNRLEKYAQESLLK
                     FAEEYHMYLENDCQPLKELFSKDFDEKLNALLAQLPEVRRQVFEMVYHEGLKAKEVSE
                     ILQMPQRTVESHIYLAMKFLRKHLSSSDFLILILF"
     gene            complement(636..1745)
                     /locus_tag="PLI30_13505"
     CDS             complement(636..1745)
                     /locus_tag="PLI30_13505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009276039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5009 domain-containing protein"
                     /protein_id="HOI80609.1"
                     /translation="MEKQQPERLKSLDALRGFDMFWIMGAEEIFVLLGSLTGLPALQW
                     WANQMTHVEWHGFHAYDMIFPLFLFIAGVSFPFSAKKRLSIDGGRKSLYRHVFKRGLL
                     LVLIGVIYNNGLNFDVANMRYASVLGRIGLAWMFAALLFMNTKNARIRLLWLSGILIS
                     YWLLFVFFKAPDLGDPDHYSMRGNISSYIDRILLPGRFCCYEIGDNEGLLPTVAAIGT
                     ALLGMLTGEFLMTKLKPTKKVLYLATAAVLLMIVGQVWNLVFPINKNLWSSSFVCWVG
                     GLSMLLLAIFYLVIDVWKFQKWAFFFVVIGMNPITIYLANRIIGWDSANRFFFGGLAG
                     LLPEAWAPFIMAIGYVTIGWVFLYILYKKKVFLKV"
BASE COUNT          560 a          427 c          326 g          468 t
ORIGIN      
        1 aaaagagtat taaaataagg aaatcggagg aagataaatg ttttcgaaga aatttcatgg
       61 caaggtaaat atggctttcc accgtacgtt gcggcatctg aagtatttcg gaaacttctt
      121 tcgctttcag accctcgtga taaaccattt cgaacacttg acggcgtact tcgggaagct
      181 gcgccaacaa agcgttcaac ttttcatcga aatctttact aaaaagttcc ttcaagggct
      241 gacaatcatt ctcgagatac atatggtatt cctcagcaaa cttcaaaagt gattcttgcg
      301 catatttctc taaccgatta ttttttttaa tgtgatcgat gcatctcgaa taagccgttt
      361 gaaaaaggta gctgagaaac cccacatcta cttgcaggct ctttcgtttt tcccacacat
      421 aggcaaatac ctcctgggca atgtcttctc ccgccgtttt attatccagg atgacggtgg
      481 catacgctgc aacctgtgga taaaaagaac aaaacaactc ttcaaactgg gattgagaaa
      541 tggcctgaag atttacagaa gaattataat cgttattatt agtcaaaaga agaaaaaaat
      601 ttaactttta tgtttcgaaa gatttccact atcgtttaaa ccttcaaaaa aactttcttt
      661 ttataaagta tgtataagaa tacccaaccg atagtcacgt aaccgatagc catgataaac
      721 ggggcccatg cttcgggcaa cagcccggca agcccgccaa agaaaaatct atttgcactg
      781 tcccatccta ttatccggtt tgccagatag atggttatgg ggttcatccc tataactaca
      841 aagaaaaacg cccatttctg aaatttccac acatcgataa ccaggtagaa aattgcgagc
      901 agcagcatac tcaatccgcc tacccagcaa acgaatgaac ttgaccacag gttcttgtta
      961 atagggaaaa cgaggttcca tacctgtcct acaatcatca atagaacagc tgctgttgca
     1021 aggtataata ctttctttgt tggtttcagc ttagtcatca agaactctcc ggtaagcata
     1081 cccagaagcg ccgtaccaat agctgcaacg gtcggcaaca gcccttcgtt gtctcctatt
     1141 tcataacaac aaaacctacc cggcaataaa atccggtcga tataactgga aatatttcct
     1201 ctcatggagt aatgatcggg atcccccagg tcgggcgctt taaaaaacac aaacaacaac
     1261 caataggaaa tcaggatccc gctaagccac agcaaccgga ttctcgcatt tttagtgttc
     1321 ataaacagca gtgcggcaaa catccaggct aacccgatcc ggccaagcac gctcgcgtag
     1381 cgcatatttg ccacatcaaa attcaatccg ttgttgtaaa taaccccgat caagaccaaa
     1441 agcaatcccc ttttaaagac atgcctgtaa agcgacttcc gtcccccatc gatactcaag
     1501 cgtttcttag ccgaaaacgg aaaagaaacc ccggcaataa acagaaacaa tgggaaaatc
     1561 atatcgtatg catgaaatcc gtgccattcc acatgtgtca tttggttggc ccaccattgt
     1621 aatgcaggga ggccggtcag cgatccaagc aaaacaaaaa tctcctccgc acccatgatc
     1681 cagaacatat caaacccgcg aagggcatcc aatgatttca gtctttccgg ttgttgtttc
     1741 tccattatgt tttgttaatt ttttcgtgtt ttgtcataat c
//
LOCUS       DAOSDR010000483         1779 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrimonas sp. isolate AMR_MDS_1826
            NODE_22513_length_1779_cov_3.801624, whole genome shotgun sequence.
ACCESSION   DAOSDR010000483 DAOSDR010000000
VERSION     DAOSDR010000483.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863355
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrimonas sp. (wastewater metagenome)
  ORGANISM  Petrimonas sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Dysgonomonadaceae; Petrimonas.
REFERENCE   1  (bases 1 to 1779)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1779)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:10:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,720
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 35
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1779
                     /organism="Petrimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22513_length_1779_cov_3.801624"
                     /isolate="AMR_MDS_1826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2023866"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..262)
                     /locus_tag="PLI30_13510"
     CDS             complement(<1..262)
                     /locus_tag="PLI30_13510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019539974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HOI80610.1"
                     /translation="MVSDKTILVTGGAGFIGSNLVEELLASGNRVICLDNFSTGKREN
                     ILSCLSHPYFTLIEGDIRDLETCRKATEGTEYVFHQAALGSVP"
     gene            complement(299..1045)
                     /locus_tag="PLI30_13515"
     CDS             complement(299..1045)
                     /locus_tag="PLI30_13515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004320794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular biosynthesis protein"
                     /protein_id="HOI80611.1"
                     /translation="MWPFSKRSLFVDSRILYGLTDYHTHLLPGVDDGISSMEEALEAL
                     SGLERHGVKRVWLTPHIMEDMPNATLDLQARFRELKAAYRGTMELHLAAEYMLDNLFW
                     ERLTRNDLLPLEGNLLLVETSCFNPTMDIEGVFESILSCGYYPVLAHPERYSYMTMND
                     LYRLKTAGVQFQLNLPSITGLYGKTIRITAGKLLQKNLYDFIGTDMHSLSHFRHVNNT
                     NISIKCIQRLRVLAVLQQYNQFGIQRPADI"
     gene            complement(1054..>1779)
                     /locus_tag="PLI30_13520"
     CDS             complement(1054..>1779)
                     /locus_tag="PLI30_13520"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019539975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004713 - protein tyrosine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0004715 - non-membrane spanning protein
                     tyrosine kinase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CpsD/CapB family tyrosine-protein kinase"
                     /protein_id="HOI80612.1"
                     /translation="KSRFSWEKWDTPAIVVKEKNRNVINEAFRLLRTNLEFMVKKESQ
                     SKVIMLTSINPNAGKTFITMNLAAGFAIKEKKVLVIDLDMRKALLSSYVQSSGPGIAD
                     YLGHQTEEWRSAIVKGNNRGEPDLLPVGTIPPNPTELLFDKRLEQMLEELRTEYDYIF
                     VDCPPVEIVADTSIINKLVDMTLFIVRVGLLDRSLLPEIENFYTSGKLRNMSLILNGT
                     MLANARYGYRYGYAYGNDYIDED"
BASE COUNT          479 a          422 c          378 g          500 t
ORIGIN      
        1 ggggaacaga gcccaaagcc gcctgatgga acacatattc cgttccttct gtcgccttgc
       61 ggcaggtttc caaatcgcgt atatcccctt ctatcaatgt aaaataagga tgggacagac
      121 aggataggat attctcccgt tttcccgtag aaaagttatc aaggcatatc accctgtttc
      181 ctgatgccag cagttcctct accagattgg agccgataaa gccggctccc ccggttacta
      241 aaatggtctt atcgcttacc atatttattt gatgcaatct ttataagtta ctcatgtttt
      301 atatatcagc aggtctttga ataccgaatt ggttgtattg ttggagtacg gccaatactc
      361 tcaggcgctg gatacatttt atactgatat ttgtattgtt cacatgccgg aaatggctca
      421 acgagtgcat atcagtgccg ataaagtcat ataggttctt ttgaaggagt ttcccggccg
      481 tgatccggat ggtctttccg taaaggcctg ttatggaagg aagatttaac tgaaattgta
      541 ccccggcagt ttttaaccgg taaagatcgt tcattgtcat gtagctgtat cgttcgggat
      601 gcgctagcac aggataatat ccacacgaca gaatggattc aaaaacaccc tctatatcca
      661 tcgtaggatt aaaacaggag gtttctacca acagcaggtt tccctccagc ggaagcaggt
      721 cgttccgggt caatctctcc cagaaaaggt tatccagcat atactccgca gccaggtgaa
      781 gttccattgt acccctgtag gcagctttca actcccggaa tcgggcctgt aggtctaggg
      841 tagcattggg catatcttcc atgatatggg gcgtaagcca cacacgtttc acgccatgcc
      901 gctctaagcc agacagtgct tccaatgcct cttccatact ggaaatacca tcatccacgc
      961 caggaagcaa atgagtatgg tagtcggtaa gtccgtacag aatacggcta tcaacaaata
     1021 aagaacgttt tgaaaagggc cacataaatt tcactaatct tcatcgatat aatcgttccc
     1081 ataggcatat ccgtaacgat atccataacg ggcattcgcc agcatggtcc cgttaagtat
     1141 taacgacatg tttcgtaatt tcccggaggt ataaaagttc tctatctcgg gcagaaggct
     1201 acggtctaac aatcctaccc gaacgataaa gagtgtcata tccactaatt tattgatgat
     1261 agaggtatca gctacaattt ctactggcgg acaatcgaca aaaatgtaat cgtattctgt
     1321 acgcaattcc tccagcatct gttccaaccg cttatcaaac aacaactcag tgggattggg
     1381 agggatagtc cccaccggaa gcagatccgg ttcgcctctg ttattcccct ttacaatagc
     1441 agacctccac tcttcggtct ggtgcccgag ataatcagcg ataccgggtc cggaggattg
     1501 aacataggaa cttaacaatg ccttgcgcat atccaaatct attaccagta cctttttctc
     1561 ctttatggcg aaaccggcgg caaggttcat ggtgataaac gtttttcctg cgttaggatt
     1621 tatggaagta agcataatca ctttggactg gctttccttt tttaccataa attcgaggtt
     1681 ggtgcgtaat aaccgaaaag cttcattgat gacattgcgg ttcttttcct tgactacgat
     1741 ggccggtgta tcccattttt cccacgaaaa cctactttt
//
LOCUS       DAOSDR010000484         1755 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrimonas sp. isolate AMR_MDS_1826
            NODE_22908_length_1755_cov_4.227647, whole genome shotgun sequence.
ACCESSION   DAOSDR010000484 DAOSDR010000000
VERSION     DAOSDR010000484.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863355
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrimonas sp. (wastewater metagenome)
  ORGANISM  Petrimonas sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Dysgonomonadaceae; Petrimonas.
REFERENCE   1  (bases 1 to 1755)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1755)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:10:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,720
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 35
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1755
                     /organism="Petrimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22908_length_1755_cov_4.227647"
                     /isolate="AMR_MDS_1826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2023866"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..473
                     /locus_tag="PLI30_13525"
     CDS             <1..473
                     /locus_tag="PLI30_13525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006843862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="isocitrate/isopropylmalate family dehydrogenase"
                     /protein_id="HOI80613.1"
                     /translation="DMVASAMKWNGGFVWACKNYDGDVQSDTVAQGFGSLGMMSSVLV
                     TPDGRTVEAEAAHGTVTRHYRQHQQGKETSTNPIASIFAWTRGLEHRGKLDGNQELID
                     FCQKLEQVCVETVENGEMTKDLAVLVYGDDVKGKYLNTQDFLAALKRNLDKKLN"
     gene            complement(540..1412)
                     /locus_tag="PLI30_13530"
     CDS             complement(540..1412)
                     /locus_tag="PLI30_13530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006800101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase"
                     /protein_id="HOI80614.1"
                     /translation="MKIGIIQQANTTDRNENVSRLQSKIRDLVSKGADLIVLQELHNG
                     LYFCQTEETGIFDQAETIPGPSTHSFGQLAKELGVVILLSLFEKRAPGLYHNTAVVIE
                     KDGTIAGKYRKMHIPDDPAYYEKFYFTPGDLGFNPIDTSVGKLGVLVCWDQWYPEAAR
                     MMALAGAEVLIYPTAIGYESSDTEDEKNRQREAWITVQRGHAVANGIPVIAVNRTGYE
                     PDPSGQTRGISFWGNSFICGPQGEFLFQAAEGEITQVVEIDKTRTENVRRWWPFLRDR
                     RIDEYGELTRRFRD"
     gene            complement(1474..>1755)
                     /locus_tag="PLI30_13535"
     CDS             complement(1474..>1755)
                     /locus_tag="PLI30_13535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012573640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004668 - protein-arginine deiminase
                     activity [Evidence IEA];
                     GO_process: GO:0009446 - putrescine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="agmatine deiminase family protein"
                     /protein_id="HOI80615.1"
                     /translation="KTTEGKPYRLIPLPMADAVYHDGYRLPATYANFLIIDDAVLMPT
                     YASAKDEMAKKQLQKAFPYREIIGVDCLPLIKQHGSLHCVTMQFPKGFI"
BASE COUNT          448 a          392 c          447 g          468 t
ORIGIN      
        1 atgacatggt ggcatcagcg atgaaatgga acggtggttt tgtgtgggcc tgtaagaatt
       61 acgacggcga tgtacagtcg gataccgtag cacagggttt cggatcgctg ggaatgatgt
      121 cttcggtttt ggtaacaccc gacggacgta cagtggaagc tgaagctgca cacggaacgg
      181 taacacgcca ttatcgtcag catcagcaag gcaaggaaac cagcaccaat ccgatcgcat
      241 caatctttgc gtggactcgc ggtttggaac acagaggaaa gcttgatggc aaccaagaac
      301 ttattgattt ctgtcaaaag ctggaacagg tgtgtgtgga aaccgttgag aacggagaaa
      361 tgaccaaaga cctggcagta ctggtatatg gcgatgatgt gaaaggaaaa tacctgaata
      421 cgcaggattt cctggctgcg ctaaagcgca atctggataa aaaattaaat tgatatacgt
      481 agttttcagg ttatcagata gaacggctca cgcggaataa cgtcgcggag ccgtttttgt
      541 taatccctga accgtcgtgt caattccccg tactcatcga tgcggcgatc gcgaaggaaa
      601 ggccaccagc gacggacgtt ttcggtgcgg gttttatcta tttcaacaac ctgagttatt
      661 tcaccttctg ctgcctgaaa taggaactca ccttgtggtc cgcagataaa gctgtttccc
      721 cagaatgaaa tcccgcgagt ctggcccgat gggtcaggtt cgtaaccggt acggttcacc
      781 gcaattactg gaatgccgtt tgctacggcg tgcccgcgct gaacagtgat ccacgcttca
      841 cgttgacgat tcttttcgtc ttcggtatcg gaggactcat agccgatagc ggtggggtag
      901 atgagcactt ctgctcccgc taaagccatc attcgggcag cctcgggata ccattggtcc
      961 cagcagacca gcacaccaag ttttcccacc gatgtatcta tggggttaaa acccaaatca
     1021 ccgggagtga aataaaattt ttcgtaatag gccggatcgt cgggtatatg cattttgcgg
     1081 tattttccgg ctattgttcc atctttttca atgaccacag ctgtattgtg atacagccca
     1141 ggagcccgtt tttcgaaaag agataacagg ataaccactc ccagctcttt tgccagttgg
     1201 ccaaatgaat gggtggaagg gcccggaatg gtctctgcct ggtcgaatat acccgtctct
     1261 tcggtctgac aaaagtacag tccgttatgc aattcttgta acacaattag gtctgctcct
     1321 ttcgatacta agtcgcggat ttttgactgc aatcgcgaaa cgttctcgtt tctgtctgtc
     1381 gtgttggctt gttgtattat tccgattttc atatgttgtc tgattggtag aaaaaatccg
     1441 cttggcgccg gcggaagaat agtaaatcct aaatcaaata aaacctttcg gaaattgcat
     1501 cgttacacag tggagtgagc cgtgttgctt gatcaaaggc agacaatcga cgccgattat
     1561 ttcacgatag ggaaatgcct tttgcagttg tttttttgcc atttcgtctt ttgcggatgc
     1621 ataagtaggc attaaaacag catcgtcgat aatcagaaaa ttggcgtaag tggcgggaag
     1681 tctatagccg tcatgataca cagcatccgc catgggtagc ggaattaacc tgtaaggctt
     1741 cccttcggtg gtttt
//
LOCUS       DAOSDR010000485         1743 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrimonas sp. isolate AMR_MDS_1826
            NODE_23134_length_1743_cov_2.725711, whole genome shotgun sequence.
ACCESSION   DAOSDR010000485 DAOSDR010000000
VERSION     DAOSDR010000485.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863355
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrimonas sp. (wastewater metagenome)
  ORGANISM  Petrimonas sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Dysgonomonadaceae; Petrimonas.
REFERENCE   1  (bases 1 to 1743)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1743)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:10:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,720
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 35
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1743
                     /organism="Petrimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23134_length_1743_cov_2.725711"
                     /isolate="AMR_MDS_1826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2023866"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..984)
                     /locus_tag="PLI30_13540"
     CDS             complement(<1..984)
                     /locus_tag="PLI30_13540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019537702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HOI80616.1"
                     /translation="MRKNFRFLTLLTGLIFSVAFLQAQEMMVKGKVHDNEGETLPGVS
                     VVVKGTTHGTVTDIDGNYELPTTSGSTLVFSYVGYQTLEVRVANSPVINVVLNASTVG
                     LDEVVVVGYGTQSRRTITSAITKVGGEVLQNVPVNTVGEGLKGKIAGTRIYSNNNTPG
                     KDATIRIRGGSSINKSNEPLILVDGVERAFGGINPNDIESIEVLKDASSTAIYGSRAS
                     NGVVLITTKKGSVGKVPSITFEANMALQEAETLYKFMNAKDYLSIVRPAVAVGPNSKY
                     NWMDGYSASSGNTASSIYSTRYLGEGEAVPTGYQSMPDPLDPTKTLIFQDNN"
     gene            complement(1070..>1743)
                     /locus_tag="PLI30_13545"
     CDS             complement(1070..>1743)
                     /locus_tag="PLI30_13545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009292517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HOI80617.1"
                     /translation="HVGSNNQREAMELARHAAVKGADGIGCIAPSFFKPEKVKDLIDF
                     FTPIASSAPQLPFYYYNMPSMTGVNLSVAQFLHEGKKTIPNLAGVKFTHNNLMEMGDC
                     IQLDNGAFEVLHGFDETLICGLALGAVAGVGSTYNYIPHVYLNILKSMESNDVETARA
                     FQMQSVEMVKIIIKYGGGVRGGKAIMNLIGVECGTCRPPLAPFEMEEYDSLKEDLRRF
                     GLFIN"
BASE COUNT          473 a          418 c          340 g          512 t
ORIGIN      
        1 attgttatcc tgaaatatca gggtttttgt tgggtccaac ggatcaggca tagattgata
       61 accggtaggc accgcctccc cttcgcccag ataacgtgta gaatagatgg aagaagctgt
      121 attgccggaa ctggccgaat aaccatccat ccagttatac ttggagttgg gtccaacagc
      181 aaccgctgga cgcacgatac tcaggtagtc cttggcgttc atgaacttat agagtgtctc
      241 tgcttcctgc aatgccatat ttgcttcaaa agtgatgctt ggaacttttc ctacagaccc
      301 ttttttggtt gtaatcaaca caacgccatt agaagctctg gaaccgtata tagcagttga
      361 cgatgcatct ttaagcactt ctattgattc tatatcgttc ggatttattc caccgaaagc
      421 cctttccact ccatcgacaa gaataagggg ttcattgctc ttattgatgg aggaacctcc
      481 gcggatccgg attgtcgcat ccttacccgg agtattgtta ttcgaataga tccgcgtacc
      541 agcgatttta cctttcaaac cctcaccaac agtatttacc ggtacatttt gtaatacctc
      601 tccacctact ttagtaatag cagatgtaat cgtacgtctt gactgggttc catatcccac
      661 aaccactact tcatcaagac ctactgtcga tgcgtttaat acgacattga taactggtga
      721 gttcgccacc cttacttcta atgtttgata acctacataa gaaaaaacca gtgttgaacc
      781 tgaagtagtg ggcaattcat aatttccgtc aatatcagtg acggtaccgt gtgtggttcc
      841 tttcacaacc acagaaacac caggtaaagt ctcaccttcg ttatcgtgca ctttaccttt
      901 aaccatcatc tcctgtgcct ggagaaaagc aactgaaaag ataagaccag ttaataaggt
      961 caaaaatcga aaattttttc tcatctttgt gttgatttta aaaataattg tgtttttaaa
     1021 ttctaattag aagagagtgt tgtttgctgg acgaactctc ttcttttctt taattaataa
     1081 acaaaccgaa tctccgaagg tcctccttca aagaatcata ctcttccatt tcaaatggag
     1141 ccaatggtgg acggcaagtt ccacattcta ctccgataag attcataatg gccttgcctc
     1201 ccctaacccc tcctccatat ttaatgatga ttttcaccat ttctaccgac tgcatctgaa
     1261 aggcacgcgc cgtttccaca tcgttacttt ccatagattt cagaatattc agataaacat
     1321 ggggtatata gttatacgta cttcccacac cggctacggc tccgagtgct aatccacaaa
     1381 ttagtgtctc gtcgaagcca tgcaacacct caaatgcccc gttatccagc tgaatacagt
     1441 cgcccatttc catcaggttg ttgtgtgtaa acttcactcc ggccaggttg ggaatagttt
     1501 ttttcccttc gtgaagaaat tgtgcaaccg aaaggtttac cccggtcatg gagggcatgt
     1561 tgtagtaata gaatggcaat tgaggagcgg aagaagcaat gggcgtaaag aaatcgatca
     1621 gatccttcac tttttcaggc ttgaaaaaag aaggggcgat acatccgatt ccatctgcgc
     1681 ctttcactgc agcatgacgt gccagctcca ttgcctcgcg ctggttattg ctgcccacat
     1741 ggg
//
LOCUS       DAOSDR010000486         1741 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrimonas sp. isolate AMR_MDS_1826
            NODE_23164_length_1741_cov_4.042112, whole genome shotgun sequence.
ACCESSION   DAOSDR010000486 DAOSDR010000000
VERSION     DAOSDR010000486.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863355
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrimonas sp. (wastewater metagenome)
  ORGANISM  Petrimonas sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Dysgonomonadaceae; Petrimonas.
REFERENCE   1  (bases 1 to 1741)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1741)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:10:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,720
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 35
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1741
                     /organism="Petrimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23164_length_1741_cov_4.042112"
                     /isolate="AMR_MDS_1826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2023866"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..780
                     /gene="rluF"
                     /locus_tag="PLI30_13550"
     CDS             <1..780
                     /gene="rluF"
                     /locus_tag="PLI30_13550"
                     /EC_number="5.4.99.21"
                     /inference="COORDINATES: protein motif:HMM:NF007784.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2604) synthase RluF"
                     /protein_id="HOI80618.1"
                     /translation="VLVNGLLIESKAEEDFVLLAFNKPRGIVSTTEKGVRDNIVDYIG
                     YHERIFPIGRLDKDSQGLILLTNDGDIVNKILRAGNSHEKEYVVTVNRPVTDEFVQRM
                     SSGVPVLGEVTRKCKVEKISEFIFRIILVQGLNRQIRRMCEYFGYEVEKLERVRIMNI
                     SLKGIGLGDWRLLEEQEMKELKTTIAYSSNENQPAKSKQPLVSGQEQERKKFRSSYEE
                     SFNSSKKAQSKRNEGFVNPKAKELRGKGKNKPQPRTFRKTK"
     gene            883..>1741
                     /gene="ychF"
                     /locus_tag="PLI30_13555"
     CDS             883..>1741
                     /gene="ychF"
                     /locus_tag="PLI30_13555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007661265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HOI80619.1"
                     /translation="MALQCGIVGLPNVGKSTLFNCLSNAKAQAANFPFCTIEPNVGVI
                     TVPDERLNRLAELVHPQKIVPTTVEIVDIAGLVKGASKGEGLGNKFLANIRETDAILH
                     VLRCFEDENVTHVDGNINPVRDKEIIDAELQLKDLETIESRIQKVEKQAKTGGDKDAK
                     LAFEVYSKIREALLRGESARTVVFDTKDENKVARDLFLLTSKPVLYVCNVDEQSAVSG
                     NKFVEQVREAVKIENAEILIVAAKIESEIAEFDTYEEREMFLVELGLEESGVNRLIKA
                     AYKLLNLQTF"
BASE COUNT          563 a          298 c          422 g          458 t
ORIGIN      
        1 gtgttggtaa acggtttgct tattgaatcc aaagcggagg aagattttgt cttgctggct
       61 ttcaataagc cacggggaat agtaagtacc accgagaaag gggtacggga caatattgtt
      121 gattatatag gttaccacga gcgaattttc cctatcggac ggttggataa agattctcag
      181 gggttgattc ttcttaccaa tgacggggat atagtgaata aaatccttcg tgcaggaaac
      241 agtcatgaga aagagtatgt tgtgactgta aacaggccgg ttaccgatga attcgtgcag
      301 cggatgtcga gtggggtgcc agtgttagga gaggtaactc gaaagtgtaa agttgaaaaa
      361 atatcggaat ttatttttcg aataattctt gttcagggac ttaatcgcca gatccgcagg
      421 atgtgcgaat attttgggta tgaagtagag aaactggaac gtgtccggat tatgaacatt
      481 tcactgaaag gtattggttt aggcgattgg cggttgttgg aagaacagga aatgaaagaa
      541 ttaaaaacga cgattgctta ttcgtctaac gaaaaccaac cggccaaatc aaaacaaccc
      601 cttgtttcgg gacaggaaca ggagagaaaa aagtttcgct cctcctatga agagtcgttt
      661 aatagttcca aaaaggctca atccaaaaga aacgaaggtt ttgtcaatcc aaaagcaaag
      721 gaattacgtg gaaaaggcaa aaacaaacct caaccaagga catttagaaa aaccaagtaa
      781 atttttgtac ctttgcaccc gcaaaaatat tcacataccg ttttcgtcaa gaagccctga
      841 tacttggctc taaaagcgta acggtctaac aatctcgaaa acatggctct tcaatgtggt
      901 atcgttgggc ttcctaatgt agggaaatca actcttttta attgtctatc aaacgctaaa
      961 gcacaggctg caaactttcc tttttgcacc attgagccca atgtgggtgt tataactgtt
     1021 ccggatgaaa gactgaaccg gttggcagag ttggtccatc cccaaaaaat tgttcccaca
     1081 accgttgaga tcgtcgatat agcaggtttg gtaaaaggag caagtaaagg ggaaggattg
     1141 ggaaataaat ttctggccaa cattcgggag acggatgcta ttttacatgt gttgcggtgt
     1201 tttgaggacg aaaatgtgac tcacgttgat ggaaatatta atccagttcg cgacaaagaa
     1261 ataatcgatg ctgaactgca gctaaaggat ttggaaacta ttgaatcaag aattcaaaaa
     1321 gtagaaaaac aagccaaaac cggcggcgac aaagatgcga agctggcttt tgaagtttat
     1381 tcaaaaatcc gtgaagcatt gctgcgagga gaatcggccc gaactgttgt ttttgatacc
     1441 aaagatgaaa ataaagtggc tcgggattta tttcttttga cgtccaaacc ggtactttat
     1501 gtttgtaatg ttgacgaaca gagcgccgta agtggaaata aattcgtaga acaggttcgc
     1561 gaggcggtaa agatagaaaa tgctgaaatt ttgatcgtgg cagcaaaaat tgagtccgaa
     1621 atagctgaat ttgataccta cgaagagcgg gaaatgtttc tcgttgaact cggtttggaa
     1681 gagtcgggtg taaaccggtt gattaaagct gcctacaaat tgttaaacct tcagacattc
     1741 t
//
LOCUS       DAOSDR010000487         1728 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrimonas sp. isolate AMR_MDS_1826
            NODE_23364_length_1728_cov_3.563658, whole genome shotgun sequence.
ACCESSION   DAOSDR010000487 DAOSDR010000000
VERSION     DAOSDR010000487.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863355
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrimonas sp. (wastewater metagenome)
  ORGANISM  Petrimonas sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Dysgonomonadaceae; Petrimonas.
REFERENCE   1  (bases 1 to 1728)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1728)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:10:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,720
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 35
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1728
                     /organism="Petrimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23364_length_1728_cov_3.563658"
                     /isolate="AMR_MDS_1826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2023866"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..657
                     /locus_tag="PLI30_13560"
     CDS             <1..657
                     /locus_tag="PLI30_13560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="SusC/RagA family TonB-linked outer membrane
                     protein"
                     /protein_id="HOI80620.1"
                     /translation="INKESTFGTNRFGNYMAYRVPGAVYVDETNVSGIPAKTMTGAYL
                     VGTDGRLNPQAELLYNDTFDKVLLNENFRQEYNISANGGTDKVDYYVSLGYLSDPSYI
                     SNSKFDRFSGRSTINSQLFKWLKVGANVGFSRTSTDKMGTTWGRNAGSNQGNVFRFIN
                     GFAPIVPVYAYNEDGSYRTHPEDGTIYNSTAGSTYSPIGETGALYGSTNIAYAMENDK
                     "
     gene            673..>1728
                     /locus_tag="PLI30_13565"
     CDS             673..>1728
                     /locus_tag="PLI30_13565"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOI80621.1"
                     /translation="MLTTRSYAEISFLNDFKFLVNFSFDKNNISQVRYANSVTGAAKD
                     QGGIYRRLTNQTIINTQEILTYNKDFDKHHVDAMAAHEFNDWRNEGINWGSSYELIPG
                     FLGSGNFVGRYVNARGFGGPGYGDDIERMESYLGRANYIYDEKYYLSTSIRRDGSSKF
                     KKNRWGTFWSLGAGWRVTSEPFMMGTKNWLDNLKLRTSYGVIGNSNAIGRYSGYRTWG
                     YGARYTETSAGTGTPNAYTMSVGGFVNDALTWENTKTFDVGIDFSLFNRVHGTVDYYN
                     RLTDNTFYNQQVNYMAVGQESLQSNSAALQNNGFEVELGVDIIRRKDFSWTISTNGTH
                     YATTLKDVPAGSIPEYTP"
BASE COUNT          531 a          388 c          406 g          403 t
ORIGIN      
        1 attaataaag aatcgacatt tgggacaaat cgatttggaa actacatggc ataccgcgtt
       61 ccgggcgcag tttatgtaga tgaaacaaac gtaagcggaa ttcccgccaa aacaatgacg
      121 ggtgcctatc tggtaggcac cgacggacga ttgaatcccc aagctgaact tctgtataac
      181 gacacattcg acaaagtact gttaaacgag aacttcaggc aggaatacaa tatttcagcc
      241 aacggaggaa ccgataaggt tgattattac gtatctcttg gttacctgtc tgacccttct
      301 tatatcagca attcaaaatt tgaccggttc agcggcagat caaccataaa ctctcagcta
      361 ttcaaatggt taaaagtagg agccaatgtt gggttttcca gaacttccac cgacaaaatg
      421 ggtaccacct ggggaagaaa cgccggctct aaccaaggga acgtatttag gtttatcaac
      481 gggtttgccc ccattgttcc cgtatatgct tacaacgaag acggcagtta cagaactcat
      541 cccgaagatg gcacaatata caattccacg gcaggaagca cctattcacc aattggagaa
      601 acaggcgcat tgtatggaag caccaacatc gcctatgcga tggaaaatga caaatagcag
      661 gatgtggctg acatgcttac tacccgatca tatgctgaaa tcagtttctt gaatgatttt
      721 aaatttttgg tgaacttctc gtttgacaag aacaatattt ctcaggtaag atacgccaat
      781 agcgtaaccg gagcggcaaa ggaccaagga gggatttaca ggaggctgac taaccaaacc
      841 atcatcaata cccaggagat cctgacttat aacaaagact ttgacaaaca ccatgtggat
      901 gccatggctg cacacgaatt caatgactgg agaaatgaag gcattaactg gggatccagc
      961 tatgaattga tcccgggatt tttgggttct ggaaactttg taggccgata cgtcaatgcc
     1021 agaggatttg gaggacccgg atacggagac gacattgaaa gaatggagag ttacctggga
     1081 cgtgccaatt acatctacga cgaaaagtac tacctctcca cttcgatccg tcgtgacggt
     1141 tcatccaagt tcaagaaaaa caggtggggt acattctggt cgttgggtgc cggttggcgc
     1201 gtaacgtccg aacccttcat gatggggacc aaaaactggc tggacaacct gaaattgaga
     1261 acgagctacg gggtaatcgg taactccaat gcaattggcc gctacagcgg ttacaggacc
     1321 tggggctacg gagcgaggta caccgaaaca tcagcgggta cgggtacacc caatgcctat
     1381 acaatgagcg taggtggttt tgtgaacgat gcattgactt gggaaaacac aaagactttt
     1441 gacgtaggaa ttgatttctc cttgtttaac cgggtgcacg gtaccgttga ctactataac
     1501 cgacttaccg acaacacatt ctacaaccaa caagtgaact atatggcagt tggccaggaa
     1561 tcactccaat ccaacagtgc agcattgcag aacaacggtt ttgaagtaga actcggggtg
     1621 gatatcattc gcagaaaaga cttctcctgg actatttcta caaacggaac acactacgcc
     1681 actacgttaa aagatgtacc ggcaggttct attccggaat atactccg
//
LOCUS       DAOSDR010000488         1721 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrimonas sp. isolate AMR_MDS_1826
            NODE_23483_length_1721_cov_3.303121, whole genome shotgun sequence.
ACCESSION   DAOSDR010000488 DAOSDR010000000
VERSION     DAOSDR010000488.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863355
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrimonas sp. (wastewater metagenome)
  ORGANISM  Petrimonas sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Dysgonomonadaceae; Petrimonas.
REFERENCE   1  (bases 1 to 1721)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1721)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:10:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,720
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 35
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1721
                     /organism="Petrimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23483_length_1721_cov_3.303121"
                     /isolate="AMR_MDS_1826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2023866"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..259
                     /locus_tag="PLI30_13570"
     CDS             <1..259
                     /locus_tag="PLI30_13570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019540794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF3109 family protein"
                     /protein_id="HOI80622.1"
                     /translation="IQVQKPISCHLYPIRLQKLSEFTAVNYNRWSICKPAVKLGKREG
                     VKIYEFLREPLIRRFGEEWYKEVCEAAKLLEEEKRADSREP"
     gene            complement(256..>1721)
                     /locus_tag="PLI30_13575"
     CDS             complement(256..>1721)
                     /locus_tag="PLI30_13575"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HOI80623.1"
                     /translation="EKQWESIVGNCDEFLYLDQAFHPAAGYISVPPFTSWIAAIIQLL
                     GGGLFWVRFFPALFGTLTVVMVWFLVEELKGGLPAKMLASVLLIFSPFSRLNILFHPN
                     AFDILVWTIIFYTLVKFITSQNNKWLLMSGVICALAINNKYTVFFLIAGLLIGLLLSS
                     QRKILTHRYFYYAAGICLLLILPNIIWQANNNLPAFHHMKALNESQLSHISHAEFLIT
                     QPRFLMFGVFPVLAAFWSFIFYKPFRQFRFIGITFLVVLTLFTLCRAKSYYALGLYPV
                     LLAFGSVYAERMIGKGRVVVFSLLGGLSMGAFLFLGPYIMPYHSPEYIAENQEFYNKM
                     GLLDWEDGSLRQLPQDFANMTGWKELAAKTREAYNQIPDKEKQSTLLFCDNYGLAGAI
                     NYYNRDKVPEAYSLSTDYIFWIPHYPVILNIIWIGPEPDSTTLNLFRSVHLKGKIENK
                     YADEYGTRIYLLSQPKTDVTPVFYKMIEEKKKAMDIF"
BASE COUNT          524 a          368 c          412 g          417 t
ORIGIN      
        1 tattcaggtg cagaaaccca tttcgtgcca cctttatccc atacgtttac agaagctgag
       61 cgaatttacg gcagtaaact acaaccgttg gtcgatatgc aagcccgctg tgaaacttgg
      121 aaaacgtgaa ggggtaaaga tctacgagtt tcttcgtgag ccgcttatac gtaggttcgg
      181 tgaagaatgg tacaaggagg tttgtgaagc ggcaaagttg ctggaagagg agaaaagagc
      241 agatagcagg gagccctaga aaatatccat tgcctttttc ttttcttcaa tcattttata
      301 aaacacgggt gttacatctg ttttcggttg gctaagaaga tagatccggg ttccgtattc
      361 atcggcatat ttattttcaa ttttgccttt caggtgaaca ctcctgaaca ggttgagggt
      421 tgtgctgtcg ggttccggac cgatccagat aatgtttaga ataactggat aatgcggaat
      481 ccaaaaaatg taatcggtac ttaacgagta ggcttccggc actttatccc tgttgtaata
      541 atttatagct cccgcaaggc cgtaattgtc acagaacagg agtgttgatt gtttctcttt
      601 atcagggatt tgattgtaag cctctcttgt ttttgcggcc agttctttcc aaccggtcat
      661 attggcaaaa tcttgaggaa gttgacgcag ggaaccatcc tcccagtcga gtagccccat
      721 cttgttatag aactcctgat tttcggcgat gtattcggga gagtggtagg gcatgatgta
      781 agggccgaga aaaaggaaag cacccatgct cagccctccc agcaacgaga ataccacaac
      841 cctgcctttc ccgatcatgc gttccgcata aacactgcca aaagccagca gaacgggata
      901 aagccccagc gcataataac tcttcgccct gcaaagggtg aacagtgtaa ggacgacaag
      961 gaaagttatt ccgatgaacc ggaattggcg aaaaggcttg taaaagataa acgaccagaa
     1021 cgcagccaag acaggaaata ctccaaacat caaaaaacgg ggttgggtaa ttaaaaactc
     1081 tgcatgactg atgtgagaca gctggctttc gttcagcgct ttcatgtgat gaaaggccgg
     1141 caggttattg ttggcttgcc agataatatt gggcaggatt agcagcaggc agattcccgc
     1201 agcataatag aaatatctat gggttaaaat tttcctttga gaagaaagaa gtaacccaat
     1261 taacaacccc gcgataagaa aaaacaccgt gtatttattg ttgatagcta gtgcacagat
     1321 aacccctgac atgagcaacc atttgttgtt ttgggaagtg ataaatttga cgagcgtgta
     1381 aaaaatgatt gtccacacaa ggatatcgaa agcgtttggg tgaaaaagga tattaagtcg
     1441 ggaaaaagga gaaaaaatca gcaaaactga ggcaagcatc ttggccggaa gccctccttt
     1501 cagttcctcc accaggaacc aaaccatcac gaccgttaac gtgccgaaga gggcaggaaa
     1561 gaaacgtacc cagaataacc ctccacccaa caactggatt attgcggcaa tccacgaggt
     1621 aaaaggaggc actgaaatgt accccgcagc cggatgaaaa gcctggtcga gatagagaaa
     1681 ctcatcgcag ttgcccacga tggactccca ttgcttctca a
//
LOCUS       DAOSDR010000489         1717 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrimonas sp. isolate AMR_MDS_1826
            NODE_23532_length_1717_cov_4.243081, whole genome shotgun sequence.
ACCESSION   DAOSDR010000489 DAOSDR010000000
VERSION     DAOSDR010000489.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863355
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrimonas sp. (wastewater metagenome)
  ORGANISM  Petrimonas sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Dysgonomonadaceae; Petrimonas.
REFERENCE   1  (bases 1 to 1717)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1717)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:10:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,720
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 35
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1717
                     /organism="Petrimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23532_length_1717_cov_4.243081"
                     /isolate="AMR_MDS_1826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2023866"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1418)
                     /locus_tag="PLI30_13580"
     CDS             complement(<1..1418)
                     /locus_tag="PLI30_13580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005821535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="von Willebrand factor type A domain-containing
                     protein"
                     /protein_id="HOI80624.1"
                     /translation="MKTTLQRIGMILFMTGFFFSVWAQEMEVSGTVYDAQGKSPLIGV
                     TVMVKGTQKGTVTDINGRYRIKAEKGNTLTFSYIGYLIQEIKVKKLQLDVYLKQDEAT
                     LDEVVVVAFGTQKKNAVAGSVMRVADQSYYLSSVIFPYPREVNTEEYEAFKENRFLSA
                     IQHPLSTFSLDVDGASYSNIRRMINQGQMPSKDVVRVEEIVNYFNYDYPQPSDGYPVR
                     IVTETAVCPWNKKHNMVRIGVKAKEIPSETLPVSNFVFLLDVSGSMFSANKLPLVKAS
                     MKLLVNNLRPKDRVAIVTYAGAAGEVLPSTSASDKQKIMEALDNLQAGGSTAGGAGIQ
                     LAYKIAEKNLVKGGNNRVILCTDGDFNVGVSSPTELESLIESKRKSGVFLTVLGYGMG
                     NYKDNKLQTLAQKGNGNHAYIDNLQEANKVLVNEFGGTMYAVAKDVKLQVEFNPNFVN
                     AYRLIGYESRLLNDEDFNDDTK"
BASE COUNT          460 a          425 c          318 g          514 t
ORIGIN      
        1 tctttggtat cgtcgttaaa gtcttcgtcg ttgagcaacc ggctttcgta gccgattaac
       61 cgataggcat tcacaaagtt aggattaaat tccacttgca gcttcacgtc cttagcaaca
      121 gcgtacatcg taccgccaaa ttcgtttacc aacactttgt ttgcttcctg caagttatcg
      181 atatatgcgt gattaccatt acctttttgt gccagcgtct gcagtttgtt atccttataa
      241 ttccccatcc cgtagccgag aacggttaaa aaaacgcccg atttccgttt gctttcaatc
      301 agcgactcca actcggttgg acttgaaaca cccacgttaa aatcaccgtc ggtacataaa
      361 atcacccggt tatttccgcc tttcactaaa tttttttccg caattttata cgccaattga
      421 attcccgcgc ctccggcggt ggagcctccg gcctgaagat tatccaacgc ttccattatc
      481 ttctgtttat ccgaagcgct tgtcgatggc aatacttcgc ccgcggctcc ggcatacgta
      541 acaatggcta cccggtcttt cgggcgcaga ttgtttacca acagcttcat tgatgctttt
      601 accaacggca gtttattggc gctgaacatt gatccggaaa catcgagcaa aaatacgaag
      661 ttgcttaccg gcaacgtttc cgaaggaatc tcttttgcct ttaccccgat gcgcaccatg
      721 ttatgctttt tgttccaggg acaaaccgcc gtttcggtaa caatccgcac cggatacccg
      781 tccgaaggct gcggatagtc gtaattgaaa taattgacga tctcctccac gcggactaca
      841 tccttcgaag gcatttggcc ctgatttatc atccgccgga tattgctgta ggatgcgcca
      901 tccacgtcca acgagaaagt agataacgga tgctgaatgg cagacagaaa tcggttttct
      961 ttaaacgctt cgtattcttc cgtatttacc tcacgaggat acggaaaaat cacagacgac
     1021 aggtaatatg actgatcagc aacacgcata acgctgcctg ccacagcatt ttttttctgt
     1081 gttccaaaag caacaacaac cacttcatcc aacgtcgctt catcttgttt cagatacaca
     1141 tccaattgta gtttttttac tttaatttct tgtatcaagt acccgatata cgaaaaagtg
     1201 agggtattgc ccttctccgc ttttatcctg tatcttccat ttatatcggt aactgtcccc
     1261 ttttgtgtac ctttaaccat cacggtcaca ccaatcagcg gcgatttgcc ttgagcatcg
     1321 tacaccgttc cgctcacttc catttcttgt gcccagacag aaaaaaaaaa tcctgtcata
     1381 aatagaatca tcccgattcg ttgtaatgtt gttttcataa ttctttcgtt tttaacatga
     1441 atactttttt gaatcttctg ccttatggat acaaatgggg atgaaattcc ataaataaac
     1501 cgataaattt cttatttttg tgaatcggga taaatgtatt gaaacagtaa attcttgaaa
     1561 accaatagga aaataacaaa cctatcgaac ggagaattgt ttgaacggta taaaaaagga
     1621 cgtcaaacaa tgtacttcca cgaattgttc cgtcgataca ttccgatgct gtacggatta
     1681 tgcctaaaat acctcggtaa tgagcccgat gcgcaag
//
LOCUS       DAOSDR010000490         1715 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrimonas sp. isolate AMR_MDS_1826
            NODE_23565_length_1715_cov_6.112651, whole genome shotgun sequence.
ACCESSION   DAOSDR010000490 DAOSDR010000000
VERSION     DAOSDR010000490.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863355
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrimonas sp. (wastewater metagenome)
  ORGANISM  Petrimonas sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Dysgonomonadaceae; Petrimonas.
REFERENCE   1  (bases 1 to 1715)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1715)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:10:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,720
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 35
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1715
                     /organism="Petrimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23565_length_1715_cov_6.112651"
                     /isolate="AMR_MDS_1826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2023866"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..941
                     /locus_tag="PLI30_13585"
     CDS             <1..941
                     /locus_tag="PLI30_13585"
                     /inference="COORDINATES: protein motif:HMM:NF025736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HOI80625.1"
                     /translation="GYVAITSVIVIVLWNRLDNPGSFLLARMLIISGMFLAYSVTSML
                     NFAPATAFVRVLFSVGLITYWYPETYEFNKIFGNLDHVFASAEQSIFGFQPALAFSKH
                     FSSEWISEAFYMGYFFYFPMMMFVILYCFFYQREKFDKLTFIFLGSFFLYYFLFIFIP
                     VTGPQFYFPVIGTENAENGFFLNVGNYFYYNSELLPVSGYGDGLFNRCVRVLQAMGER
                     PTAAFPSSHVGISTILMMWLYRNNKRIMFIVFPLYALLCGATVYIKAHYLIDVFAGFM
                     SAFAFYGLFALSFDKLYGRRGSVETGSPNPTKQTTI"
     gene            1062..1643
                     /locus_tag="PLI30_13590"
     CDS             1062..1643
                     /locus_tag="PLI30_13590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005945048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin family protein"
                     /protein_id="HOI80626.1"
                     /translation="MMKSVKGTQTEQNLLKSFAGESQARMRYTFFAKQAKTEGYEQIS
                     AIFLETADQEMVHAKRMFKYLEGGMLEITATYPAGMIGTTAENLKAAAEGENEEWTDL
                     YPHFAKVAEEEGFKEIATMYRMIAKAEAVHEEKYLKLLQRVQDGTVFVREEEIEWYCR
                     HCGYVFTGKKPPKNCPTCLHPQAYFEPKNSADY"
BASE COUNT          467 a          367 c          380 g          501 t
ORIGIN      
        1 ccggttatgt cgcaattacc tctgtcattg tcatcgttct ctggaacaga ctggataatc
       61 cgggttcctt cttgttggct cgtatgctaa ttatcagcgg aatgtttctt gcctattccg
      121 tcacgtctat gcttaacttt gcaccggcaa cggctttcgt ccgtgtcctc ttctccgtag
      181 gacttatcac ttactggtat cctgaaacct acgaattcaa taaaatattt gggaacctgg
      241 accacgtttt tgcttccgca gagcaatcta tctttgggtt tcagccggcc ctggcctttt
      301 cgaagcattt ttcctcggag tggattagcg aagcattcta catggggtat ttcttctatt
      361 ttcccatgat gatgttcgtc attttgtact gtttctttta tcaacgggag aagttcgaca
      421 aattaacttt catattcttg ggatcgtttt tcctttatta tttcttgttt atctttattc
      481 ccgtcaccgg cccgcaattt tattttccgg ttataggaac agagaatgca gaaaacggat
      541 tttttttgaa tgttggcaat tatttttatt acaacagcga actgttgccc gtttccggtt
      601 atggggatgg attattcaac cgttgtgtca gggtattgca ggcaatggga gaacggccca
      661 ctgccgcatt tccaagttcg cacgtgggca tatccactat actgatgatg tggttatata
      721 gaaataataa aagaataatg ttcattgttt tcccgttata cgctcttctc tgcggagcaa
      781 ccgtatatat caaagcgcat tacctgattg atgtttttgc cggtttcatg agtgccttcg
      841 ctttttatgg tttgttcgcg ttgtctttcg ataagctata tggccgccgg ggttcggttg
      901 agaccggaag cccaaatccg acaaaacaga caactattta gacataattt aaattttgac
      961 ctgcagataa gttgagcgct aaaacggtta tttaattttg tatctttgtg agagtcttat
     1021 tctgctactc aatcagaatc taacatttta taaaacaacc aattatgaaa agtgtaaagg
     1081 gaacccaaac agagcagaac ctgctgaaat cttttgcagg cgaatctcag gcaagaatgc
     1141 gctatacatt cttcgcaaaa caggccaaaa cagaagggta tgagcaaatt tcggccattt
     1201 tcctggaaac cgcagaccag gagatggtgc acgcaaaacg tatgtttaaa tacctggaag
     1261 gcggaatgct tgaaattaca gcaacttatc cggcaggaat gatcggcaca actgcggaaa
     1321 acctaaaagc cgccgctgaa ggcgagaacg aagagtggac cgatctctat cctcatttcg
     1381 ccaaagtggc cgaagaagaa ggattcaagg aaattgccac catgtaccgg atgattgcca
     1441 aagcggaagc agtccacgag gaaaaatacc tgaagctact gcaaagggta caggacggga
     1501 ccgtttttgt acgcgaggaa gagatcgaat ggtattgccg ccactgcggt tatgtgttta
     1561 caggcaagaa accgccgaaa aactgtccga cctgcctgca tccgcaagcc tatttcgaac
     1621 ccaaaaacag tgcggattat tgatagagaa tgtgatgcga aaaatggcaa aataaaaaaa
     1681 gcgctacgaa gagcgctttt ttttgtgacc ccgac
//
LOCUS       DAOSDR010000491         1701 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrimonas sp. isolate AMR_MDS_1826
            NODE_23822_length_1701_cov_1.857837, whole genome shotgun sequence.
ACCESSION   DAOSDR010000491 DAOSDR010000000
VERSION     DAOSDR010000491.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863355
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrimonas sp. (wastewater metagenome)
  ORGANISM  Petrimonas sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Dysgonomonadaceae; Petrimonas.
REFERENCE   1  (bases 1 to 1701)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1701)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:10:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,720
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 35
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1701
                     /organism="Petrimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23822_length_1701_cov_1.857837"
                     /isolate="AMR_MDS_1826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2023866"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            67..480
                     /locus_tag="PLI30_13595"
     CDS             67..480
                     /locus_tag="PLI30_13595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007215543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FdtA/QdtA family cupin domain-containing
                     protein"
                     /protein_id="HOI80627.1"
                     /translation="MKKTNIYDCSIIEIDKHHHEKGNISVVENGKTVPFHVKRVYYLY
                     DVPGGESRGGHAHKELQQFIVAASGSFDVTLDDGELKRTFTINRPYRGLLVVPGIWRE
                     LDNFSSGSVCLVLASSEYSADDYIRDYNEFKEYKK"
     gene            477..1019
                     /locus_tag="PLI30_13600"
     CDS             477..1019
                     /locus_tag="PLI30_13600"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005937280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HOI80628.1"
                     /translation="MRYIHPRTDVLSQNIGEDTTIWQFCVILPDAQIGSNCNICAHVF
                     IENKVVIGNNVTIKSGVQLWDGITIEDNVFIGPNVTFTNELIPRSKAHDTSKFKPTLV
                     KKGASIGANATILPGLVIGEYSFIGAGSIITKDIPDYSFWYGNPATQKGYITEEGILL
                     DMDKRDKNGVKHILKIDTDD"
     gene            1012..>1701
                     /locus_tag="PLI30_13605"
     CDS             1012..>1701
                     /locus_tag="PLI30_13605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005802405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HOI80629.1"
                     /translation="MIKFLDIQKITQKYTGEIHQAASRVIDSGWYLLGEENKRFENNY
                     AGFIGTRYCVGVANGLDALRIILKAYMELGIMSEGDEIIVPANTFIASILAITDNRLV
                     PVLVEPDIRTYQLDDTKIEAAISPRTRGIMIVHLYGQCAYTDRIGEICKKHDLKLIED
                     NAQASGCKFNGKTTGSLGDAAGHSFYPGKNLGAFGDGGAITTDDKKLEETARALANYG
                     SNTKYIFDYQGL"
BASE COUNT          531 a          379 c          405 g          386 t
ORIGIN      
        1 tggcatccat caggttcgat gtagccgact atgtatatga ctacgaattg tttaaaaaaa
       61 tggcaaatga aaaaaacgaa catctatgat tgttctataa tagaaataga caagcaccat
      121 cacgagaaag gaaacatcag tgtagtggaa aacggcaaaa cggttccttt tcatgtaaag
      181 cgtgtgtatt acctctacga tgtgccaggg ggagagtcac ggggtggcca cgcacacaaa
      241 gagttgcagc aattcatcgt agcagccagc ggcagtttcg atgtaacact ggatgatggc
      301 gaattgaaaa ggacattcac cattaatcgt ccctatcgcg gattacttgt tgttcctggc
      361 atctggaggg agctggacaa tttttcttcc ggatcagttt gcctagtttt agcctcttcg
      421 gaatattctg ccgatgatta tataagagat tacaacgaat ttaaagaata taaaaaatga
      481 gatatatcca tccgcgaacg gacgttcttt cccaaaacat aggcgaagac acaacaatct
      541 ggcaattttg cgtgatattg cccgacgcac agatcggaag caactgcaat atttgcgcgc
      601 atgtattcat cgaaaacaag gttgtgatcg gtaacaacgt gaccatcaaa agcggggtac
      661 agttgtggga cgggataacc attgaagaca acgtatttat cgggcccaac gtgacattca
      721 ccaacgagct gattccgcgg tcaaaagcgc acgacacctc aaaatttaaa cccaccctgg
      781 tgaaaaaagg agcgtccatt ggcgcaaacg caacgatact tcccggactc gtgattggag
      841 aatattcctt tatcggcgcc ggaagcatca tcacgaagga cattcccgat tattcgtttt
      901 ggtatggaaa tccggctact caaaaagggt acataaccga agagggtatt ttactggata
      961 tggacaaaag agacaagaat ggagtaaagc atatattaaa gatcgacaca gatgattaaa
     1021 tttctcgaca tacagaaaat tacacagaaa tacaccgggg aaatccatca agccgcatcc
     1081 cgggtaatcg attcgggctg gtacctgctt ggcgaagaaa ataaacgatt cgagaacaac
     1141 tacgccggat ttatcggtac ccgttattgc gtgggcgttg ccaacggtct ggacgccttg
     1201 cggataatcc tcaaggcata catggaatta ggcatcatga gcgagggaga cgaaattatt
     1261 gtacccgcaa acacgttcat tgcttctatc ctcgccatta ccgacaaccg gctagttccg
     1321 gtgttggtag aaccagatat ccgtacgtat cagctggacg acaccaaaat tgaagcggcc
     1381 atctccccca gaacaagagg gatcatgatc gtccaccttt acgggcaatg cgcgtacacc
     1441 gacagaatcg gagagatctg taaaaaacac gatttaaaac ttattgaaga taatgcccag
     1501 gcaagcggat gcaagtttaa cggaaagacg acaggctctt tgggagacgc ggccggccac
     1561 agcttttatc ccggcaagaa cctgggggct tttggcgatg gcggtgccat tactaccgac
     1621 gacaaaaaac tggaggaaac ggcacgggca ttggccaatt acggttccaa caccaaatac
     1681 atattcgatt accaggggct t
//
LOCUS       DAOSDR010000492         1697 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrimonas sp. isolate AMR_MDS_1826
            NODE_23871_length_1697_cov_4.741778, whole genome shotgun sequence.
ACCESSION   DAOSDR010000492 DAOSDR010000000
VERSION     DAOSDR010000492.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863355
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrimonas sp. (wastewater metagenome)
  ORGANISM  Petrimonas sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Dysgonomonadaceae; Petrimonas.
REFERENCE   1  (bases 1 to 1697)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1697)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:10:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,720
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 35
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1697
                     /organism="Petrimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23871_length_1697_cov_4.741778"
                     /isolate="AMR_MDS_1826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2023866"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..388
                     /locus_tag="PLI30_13610"
     CDS             <1..388
                     /locus_tag="PLI30_13610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019539166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HOI80630.1"
                     /translation="LILALVGNIHFDFSGLSLRQLFRAIMVVFAGIGMGLALIIYMSS
                     RIGKPGIFSRVALVSDQEGYVSVPMEPLTLVGQTGIASTVLRPSGKIRIGDQHYDAVS
                     MKGFIEKGDEVVVKRYENFQLYVMKK"
     gene            complement(392..841)
                     /locus_tag="PLI30_13615"
     CDS             complement(392..841)
                     /locus_tag="PLI30_13615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008022371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction enzyme HsdR N-terminal
                     domain-containing protein"
                     /protein_id="HOI80631.1"
                     /translation="MYALNLPTFDAKIRKTPNGLEIFDPLRRKYVSLTPEEWVRQHFV
                     HYLISEKGYPASLMANETMIRLNSLTKRCDTVVYDNSLRPVVICEFKKPDIEITQQVF
                     DQVVRYNIVLKVKYLIVSNGMSHYCCKMNYEDMSFDYLQEIPEYGDL"
     gene            892..1668
                     /locus_tag="PLI30_13620"
     CDS             892..1668
                     /locus_tag="PLI30_13620"
                     /EC_number="3.2.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004295947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008714 - AMP nucleosidase activity
                     [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA];
                     GO_process: GO:0044209 - AMP salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AMP nucleosidase"
                     /protein_id="HOI80632.1"
                     /translation="MRTKQEIVENWLPRYTKRSLEDFTKFILLTNFQKYVEIFAHHFN
                     VPILGLDANMPSASSDGVTIINFGMGSANAATIMDLLSAISPTAVLFLGKCGGIKAQN
                     ELGDYILPIAAIRGEGTSNDYLPPEVPSLPAFSLMRAVSSNIRDFGRDYWTGTVYTTN
                     RRVWEYDDEFKNYLRKLRVMAVDMETATLFTCGFANHIPTGALLLVSDQPMISTGVKT
                     DKSDKVITENFVEEHVKIGIKSLTTLINRGSTVKHLRFDW"
BASE COUNT          484 a          347 c          375 g          491 t
ORIGIN      
        1 tttaatactt gctctggtcg ggaacatcca tttcgatttc agcggtttat ctctcaggca
       61 actgtttcgt gcgataatgg ttgtttttgc aggaatagga atggggttgg ccctgataat
      121 ctatatgtcg agccggatcg gaaaaccggg aattttcagc cgcgttgcct tggtttccga
      181 tcaggaagga tatgtttctg tgcctatgga accgctgaca ttagtcggac aaaccggaat
      241 agcctctact gtgcttcgtc cgtccggaaa aataaggata ggagaccagc attacgacgc
      301 ggtatcgatg aaaggattta tagaaaaggg agacgaagta gttgtaaaac gttacgaaaa
      361 cttccagctt tatgtgatga agaaataaac gctataaatc cccgtactcc ggtatttcct
      421 gtaaataatc aaacgacata tcttcgtagt tcattttaca gcaatagtga gacatcccgt
      481 tggatacaat caggtatttc actttcaata cgatattgta tctcactacc tgatcgaaaa
      541 cctgttgagt aatctctata tcgggtttct taaattcaca gataaccacc ggacggaggc
      601 tgttgtcata cacaacggta tcgcatcgtt tggtgagcga gtttaacctg atcatcgttt
      661 catttgccat taaagaagct ggatatcctt tctctgaaat caggtaatgc acaaaatgct
      721 ggcgtaccca ctcttcgggg gtcaaggata catatttcct gcgtaacgga tcgaaaattt
      781 ccaaaccgtt aggggttttt cggattttag catcgaaagt tggcaaattt aacgcataca
      841 tatgttatct ttgttcatcg ggaaaagtga tcagacaaaa gtaaattaaa tatgagaacc
      901 aaacaagaaa ttgttgaaaa ttggcttcca cgttatacaa agcgctcttt ggaagatttc
      961 accaaattca ttctgttgac caattttcaa aaatatgtcg aaatttttgc ccatcatttt
     1021 aacgttccta tcttgggact ggatgccaat atgcccagcg catcgtccga tggagttacc
     1081 atcattaatt ttggtatggg gagcgccaat gccgcgacta tcatggattt actgagtgcg
     1141 atttctccaa cagcggtgtt gtttctggga aaatgcggcg gaataaaagc acaaaatgaa
     1201 cttggtgact atatattacc tattgctgct attcgcggag aaggaacctc aaacgattat
     1261 cttccacccg aagtgccttc attgccggca tttagcctta tgcgtgccgt ttcttcaaat
     1321 atccgggatt ttgggcgtga ttactggacc ggaacagttt acaccacgaa tcgtcgcgtg
     1381 tgggaatatg acgatgaatt caaaaactac ctgcggaaat tgcgcgtaat ggctgtggac
     1441 atggaaacgg caacattatt cacgtgcggc tttgccaatc atattcctac gggtgccctg
     1501 ctgttggttt cggaccaacc catgatttct acaggcgtaa agaccgataa aagcgataag
     1561 gtcattaccg agaatttcgt ggaagaacac gttaagattg gcattaaatc gcttaccaca
     1621 cttatcaaca gaggttcaac ggtaaaacat ctccggtttg actggtaatg atgacggatg
     1681 ctgaaatctt tatatct
//
LOCUS       DAOSDR010000493         1684 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrimonas sp. isolate AMR_MDS_1826
            NODE_24066_length_1684_cov_2.817680, whole genome shotgun sequence.
ACCESSION   DAOSDR010000493 DAOSDR010000000
VERSION     DAOSDR010000493.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863355
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrimonas sp. (wastewater metagenome)
  ORGANISM  Petrimonas sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Dysgonomonadaceae; Petrimonas.
REFERENCE   1  (bases 1 to 1684)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1684)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:10:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,720
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 35
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1684
                     /organism="Petrimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24066_length_1684_cov_2.817680"
                     /isolate="AMR_MDS_1826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2023866"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..532)
                     /locus_tag="PLI30_13625"
     CDS             complement(<1..532)
                     /locus_tag="PLI30_13625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019538410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4294 domain-containing protein"
                     /protein_id="HOI80633.1"
                     /translation="MIRFVQIWLLLTVPLAFQAKAQDFFADNASPAKQRTTLYLLPNV
                     YPAVVIDGDTVACMSLHDFIKYSPLRFGSNREQLQYTKLIRDVKKTLPYAKEIAVIIT
                     ETYEYMETLPDDKARQSHLNQMEKYLMEAYTPKMKKLTRSQGQLLMKLVDRETNSSSY
                     HIIDAYMGSAKAFFYNA"
     gene            740..>1684
                     /locus_tag="PLI30_13630"
     CDS             740..>1684
                     /locus_tag="PLI30_13630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005635620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HOI80634.1"
                     /translation="MAYYHLGELVHHQAQKYKNRTSLKFLLSDGRWTQMSWEEFSEKI
                     MKTAQAMAELQIQPEDNVGIYSQNMAKYLITDFAAYANCAVMVPMYATSSPSQVEYIV
                     NDAQLKVIFVGEQFQYNNAYKVQQESPVLKKIIVFDRNVVLKPEDKTSIYFDDFITTG
                     DNSESLALVNARLRQLKGSDLATIIYTSGTTGEPKGVMLTHDNYIQAFKIHDARLTQC
                     SEKDVSMCFLPLTHIFEKAWTYYCLHKGILVAVNRDPTRIQQTLRQIRPTLMSNVPRF
                     WEKVYDGIQQKIETSSGALKWLFEDSVRTGRKHNLVYKN"
BASE COUNT          479 a          356 c          392 g          457 t
ORIGIN      
        1 acgcattgta aaaaaaagcc ttggcgcttc ccatgtaggc atcaataatg tggtacgacg
       61 atgagttggt ctcccgatca accagcttca tcaaaagttg tccttgtgaa cgggtcaatt
      121 ttttcatttt gggggtatat gcttccatca ggtacttttc catctggttc aggtgactct
      181 gccgtgcctt gtcatcgggc agggtttcca tatactcgta cgtttcggta atgattacgg
      241 ctatctcctt ggcatagggc agggttttct ttacgtcgcg gatcaatttt gtatattgta
      301 gctgctccct gttgctgccg aaacgcaggg gggaatactt gatgaaatcg tgtaagctca
      361 tgcaggcaac cgtgtcgccg tcaatcacca ctgccggata cacattgggc aacaggtaga
      421 gggtcgtcct ttgttttgcc ggagaagcgt tgtcggcaaa gaaatcctgc gccttggcct
      481 ggaaggcaag gggcacggtc aggagcagcc atatttgaac aaatcttatc ataaatccgg
      541 gacaaaaata acattattgt tttattcgag tacttaaaat cacgttaagt gtagttaatt
      601 aacaatcctg tttatagatt gttaagtaaa taatttggaa aaaacagctt aaccagggcc
      661 ttctcgggca aaaaagagat cgctttttac tcgatttcct tcaatttgaa ttacctttga
      721 agaactaaaa tcagaaatta tggcttatta tcatttgggg gagctggttc accatcaggc
      781 gcagaaatac aaaaacagaa cctcgttgaa gtttttatta tcagatgggc gatggacaca
      841 aatgtcgtgg gaagagtttt cggaaaaaat aatgaaaacc gctcaggcaa tggcagagtt
      901 gcagatccaa cccgaagata atgtggggat ttattcccag aatatggcaa aataccttat
      961 taccgacttt gccgcctacg ccaactgcgc ggttatggta cccatgtatg ccacctcatc
     1021 gccatcgcag gtagaatata tcgtaaacga cgctcagcta aaggtgatat ttgtaggtga
     1081 gcagttccag tacaacaacg catataaagt acagcaggaa agtccggtcc tgaaaaagat
     1141 cattgtcttt gacaggaatg tagtgttgaa acccgaagat aaaacgtcca tctattttga
     1201 tgattttatc actaccggag ataattccga atcgttggcg cttgtcaatg cccggttgcg
     1261 tcaacttaaa ggttccgatc tggcaaccat catttatacg tcgggaacaa ccggtgagcc
     1321 caagggagtt atgctcaccc atgataatta tatccaggcg ttcaagatcc acgatgcgcg
     1381 gctaacccag tgttcggaga aagatgtttc gatgtgtttc ctgccgttga cgcatatctt
     1441 tgaaaaagcg tggacctatt attgcctcca caaaggaata cttgttgccg taaacaggga
     1501 tcccacccgg attcagcaga ctcttagaca gatacgtccc actctgatga gcaacgtccc
     1561 ccggttctgg gaaaaagtgt acgatggcat tcagcaaaag atcgaaactt caagcggtgc
     1621 tttgaaatgg ttgtttgaag atagcgtcag gacgggtaga aagcacaatc tcgtatataa
     1681 aaat
//
LOCUS       DAOSDR010000494         1679 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrimonas sp. isolate AMR_MDS_1826
            NODE_24164_length_1679_cov_2.065271, whole genome shotgun sequence.
ACCESSION   DAOSDR010000494 DAOSDR010000000
VERSION     DAOSDR010000494.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863355
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrimonas sp. (wastewater metagenome)
  ORGANISM  Petrimonas sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Dysgonomonadaceae; Petrimonas.
REFERENCE   1  (bases 1 to 1679)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1679)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:10:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,720
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 35
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1679
                     /organism="Petrimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24164_length_1679_cov_2.065271"
                     /isolate="AMR_MDS_1826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2023866"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(93..1382)
                     /locus_tag="PLI30_13635"
     CDS             complement(93..1382)
                     /locus_tag="PLI30_13635"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HOI80635.1"
                     /translation="MKKVLILLGFCLLFNTQADAQKWLNKLGNAAKDAAKNAVERRVE
                     QKAEGVTEKAMDKAEESVTKEDKNKKSASNEEEEEGAIVTEEKGENMAANTSQKLTGT
                     SQYDFVPGDQILFYDDFSQDAIGDFPALWTTNGSGEVKSVNIAPGKWFHMNGEDAVYC
                     YTRQIDFPDNFIVEFDIIPDEKYQYGIQFTLYQENADDPKEMNDDLYPGEAGLHVDLG
                     YDRWDTKGYKETQDWLTGQATKNTVVREKENHVILWVQKRRVRIYHQNAKVLDMPTNI
                     YPNVKFNRMRFSGWDRYSAPYVSNLKITTASPDTRSKLITEGKLVTYGITFDVNKADV
                     KPESFGTLKSIADVLKENGPVRVKIIGHTDSDGDDAKNLELSQRRAQSVKNELIGPFG
                     IDASRIETEGAGETTPVAPNDTPANKALNRRVEFVKQ"
     gene            complement(1411..>1679)
                     /locus_tag="PLI30_13640"
     CDS             complement(1411..>1679)
                     /locus_tag="PLI30_13640"
                     /inference="COORDINATES: protein motif:HMM:NF016764.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="YIP1 family protein"
                     /protein_id="HOI80636.1"
                     /translation="IKNFDMAFSLVAYSYTPMCVAGILLLVPSLSFVATLGGLYGLYL
                     LFLGLAPMMKTPEEKKNTYFIVSLLCVVVVSVLVSVVLGALIVS"
BASE COUNT          368 a          427 c          371 g          513 t
ORIGIN      
        1 ttcatgatgt tttttttagt tagagtttaa caatttaact ccgttgattt gtttcacgat
       61 taggtaaaat tcaaacaagt ttgattttgc tctcactgct tcacaaattc aaccctgcga
      121 ttcaacgcct tgttggccgg tgtatcgttg ggtgcaacgg gagtggtttc cccggctccc
      181 tccgtttcta tccgcgacgc gtctattccg aatgggccga tcagctcgtt ctttaccgat
      241 tgggcacggc gttgcgatag ctccaggttt ttggcatcat ctccatcgct gtcggtatgc
      301 ccgataattt taactcttac cggtccattt tcctttaaca catcggcgat gctcttcagt
      361 gtgccgaacg attcgggttt tacatccgcc ttgttcacat caaaggtgat gccgtaggtg
      421 accagctttc cttcggtaat cagtttgctg cgggtgtcgg gcgatgctgt ggtgattttc
      481 aggttcgaca cgtagggtgc actgtatcgg tcccatcccg agaatcgcat gcggttgaac
      541 ttcacgttgg gatagatgtt ggtgggcata tccaacactt tagcgttctg atgatagata
      601 cgcaccctgc gtttctgtac ccagaggatg acgtgatttt ctttttcacg gacaaccgta
      661 tttttcgttg cctgtccggt aagccagtcc tgtgtttcct tgtaaccctt ggtatcccag
      721 cggtcatatc ccagatcaac atgtagccct gcttcacccg gatataaatc gtcgttcatc
      781 tctttcgggt catctgcatt ttcctggtag agtgtgaact ggattccgta ttgatatttt
      841 tcatcgggga taatgtcgaa ctcaacgata aagttatccg gaaaatcgat ctgacgggta
      901 taacagtata ccgcatcttc cccgttcata tgaaaccact tgccgggagc gatattcacc
      961 gacttcacct caccactgcc gtttgttgtc caaagggccg gaaagtcgcc gatagcatcc
     1021 tgtgagaaat cgtcgtaaaa gaggatctga tcgcccggca caaaatcgta ctggctggtg
     1081 cccgttaatt tttgcgaagt gttggcggcc atattttcgc ctttctcctc cgtaacaatg
     1141 gcaccttcct cttcttcttc gtttgaggcg gatttcttgt ttttatcctc tttcgtcacc
     1201 gactcttccg ctttatccat tgctttctcg gttaccccct ccgctttctg ttccacgcgt
     1261 ctctctacgg cattctttgc tgcatctttt gccgcatttc ccagtttatt cagccatttt
     1321 tgtgcatcgg cctgcgtgtt aaacaacagg caaaaaccta acaagataag tacttttttc
     1381 atgatctgtc tattttaaaa ttaaacttgc ttatgaaacg atcagtgcgc ccaggactac
     1441 agaaacgagg accgaaacca ccactacgca gagcaggctc acgatgaaat aggtattttt
     1501 cttctcttcg ggtgtcttca tcatgggtgc gaggcccagg aagagcaggt agagcccata
     1561 caatcctccc agcgttgcca caaacgacaa actgggaacc aacaagagta tccctgccac
     1621 gcacataggc gtataggagt aggcgaccaa agagaatgcc atatcaaaat ttttgatgg
//
LOCUS       DAOSDR010000495         1650 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrimonas sp. isolate AMR_MDS_1826
            NODE_24706_length_1650_cov_4.257680, whole genome shotgun sequence.
ACCESSION   DAOSDR010000495 DAOSDR010000000
VERSION     DAOSDR010000495.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863355
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrimonas sp. (wastewater metagenome)
  ORGANISM  Petrimonas sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Dysgonomonadaceae; Petrimonas.
REFERENCE   1  (bases 1 to 1650)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1650)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:10:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,720
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 35
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1650
                     /organism="Petrimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24706_length_1650_cov_4.257680"
                     /isolate="AMR_MDS_1826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2023866"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..204)
                     /locus_tag="PLI30_13645"
     CDS             complement(<1..204)
                     /locus_tag="PLI30_13645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005929537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C1 family peptidase"
                     /protein_id="HOI80637.1"
                     /translation="MHKLTLIVVALFISVSLFSQKSVYTFTVVKENPITSVKNQASTS
                     TCWSFSGVSFLESELLRKGKGTFD"
     gene            343..792
                     /locus_tag="PLI30_13650"
     CDS             343..792
                     /locus_tag="PLI30_13650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019538672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI80638.1"
                     /translation="MNDLQPKKRRSRKWFGRFIGIILIAFTIFVYVRYYYVFGTGVKS
                     GELNYLVYKGVIFKTYEGKLIQTGFRADKPGGLQSNQFDFSVENKEIAEKLMLSSGKN
                     VQLHYKEFFAPIPWRGYTKFIVDSIISIEDPHPDLQHEIAPYVLDTM"
     gene            800..1423
                     /locus_tag="PLI30_13655"
     CDS             800..1423
                     /locus_tag="PLI30_13655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009018726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease H family protein"
                     /protein_id="HOI80639.1"
                     /translation="MAKKKFYVVWQGVQPGIYSSWDECKAQVTGFEQARYKSFETKEE
                     ADAAFNDNPWKHIGKTGPKNRKIATSQEKYLTESIAVDAACSGNPGLMEYRGVFVADG
                     VEIFHVGPLKDGTNNIGEFLAIVHALALLKHKNSKLPVYSDSVNAMKWVVRKKCNTKL
                     EKTKYNQPIFELITRAEKWLSENTYENPVIKWETNNWGEIPADFGRK"
     gene            complement(1452..>1650)
                     /locus_tag="PLI30_13660"
     CDS             complement(1452..>1650)
                     /locus_tag="PLI30_13660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI80640.1"
                     /translation="PIESLFCETDEDFMDIRDVYAQAAQAVNMELEEFAEKIAQNIHR
                     IFPTLKAPKPFDPEEEEEEED"
BASE COUNT          517 a          340 c          380 g          413 t
ORIGIN      
        1 gtcgaatgta ccttttcctt tccgcaatag ttcagattcc aggaagctca ctcccgaaaa
       61 gctccagcag gtgcttgtgc ttgcctgatt ttttaccgat gttatagggt tttctttaac
      121 tacagtaaat gtgtaaactg atttttggga gaaaagagaa accgaaataa aaagagcaac
      181 aacaattaaa gttaacttat gcatacgaca attttttggg ttgtaatagt tgatgtacaa
      241 agttaactta aaaagagaga tggggcttta tttttggggc aataaatagc taaaaaagca
      301 aaaaaaacgt acctttggcg aagaatcgaa aactgaattt atatgaacga cctacaaccc
      361 aaaaagagaa gaagcaggaa atggttcgga cggtttatcg gaatcatcct gattgcgttc
      421 accatatttg tatatgtgcg ttattattat gtttttggca cgggcgttaa atcaggtgaa
      481 ttgaattacc tggtatataa aggggtgatt ttcaagacat acgaagggaa acttatccag
      541 accggattca gggcagacaa accgggagga ctacaatcca accaattcga tttttcggta
      601 gaaaacaagg agatcgccga aaagttgatg ctctcaagcg ggaagaacgt acaactgcat
      661 tacaaggaat tttttgcacc catcccctgg cgcggctaca cgaagttcat agttgacagc
      721 atcatttcca tcgaagatcc ccaccccgat ctccagcatg agattgcacc gtatgtttta
      781 gacacgatgt aacgaggtaa tggctaaaaa gaaattctat gtagtttggc agggtgttca
      841 gccgggaatt tactcgtcgt gggacgagtg caaggcacag gtgacgggtt ttgaacaggc
      901 ccggtacaaa tcatttgaaa cgaaggaaga agcagatgcg gctttcaacg acaatccctg
      961 gaagcacatc ggaaaaacag gccccaaaaa ccggaaaata gccacgtctc aagaaaagta
     1021 tcttacggaa agtatcgcgg tggatgctgc ctgcagtgga aatcccggat taatggaata
     1081 ccggggtgtt tttgtcgcag acggagttga gatttttcac gttggacccc tgaaagacgg
     1141 caccaacaat atcggcgagt ttttagctat cgttcacgca ctggcattgt tgaaacataa
     1201 aaacagcaaa ctgccggttt attccgatag cgtgaatgcc atgaagtggg tggtcagaaa
     1261 aaagtgcaac accaagttag aaaaaaccaa gtataaccaa cccattttcg agcttatcac
     1321 ccgggccgag aagtggttat cggaaaacac gtatgaaaac ccggttatca aatgggaaac
     1381 caacaactgg ggggaaattc cggcagattt tggaagaaaa taatccagcc agccagccgc
     1441 ctcatcaccc atcaatcctc ctcttcttcc tcttcttccg gatcaaaagg cttgggcgct
     1501 tttagcgttg ggaaaatacg gtgtatattt tgtgcaattt tttcggcaaa ctcttcgagc
     1561 tccatattca ctgcctgggc agcctgagca tacacgtcac ggatatccat aaaatcctca
     1621 tcggtttcgc aaaaaaggct ttcgatggga
//
LOCUS       DAOSDR010000496         1608 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrimonas sp. isolate AMR_MDS_1826
            NODE_25523_length_1608_cov_10.897618, whole genome shotgun
            sequence.
ACCESSION   DAOSDR010000496 DAOSDR010000000
VERSION     DAOSDR010000496.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863355
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrimonas sp. (wastewater metagenome)
  ORGANISM  Petrimonas sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Dysgonomonadaceae; Petrimonas.
REFERENCE   1  (bases 1 to 1608)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1608)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:10:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,720
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 35
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1608
                     /organism="Petrimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25523_length_1608_cov_10.897618"
                     /isolate="AMR_MDS_1826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2023866"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            121..1206
                     /locus_tag="PLI30_13665"
     CDS             121..1206
                     /locus_tag="PLI30_13665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007754517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HOI80641.1"
                     /translation="MNTRQSNKLDFKGQNIYIGIDVHLKSWSVSVLSEHSVLKRFSQS
                     PSPESLHKFLTTHYPGADYHSVYEAGFSGFWIHEKLVTLGINNIVVNPADVPTMSKEK
                     LRKTDAVDCGKLARELRSGTLKGIYVPDIATLEMRSLIRLRNRIVKDTTREKNRIKSF
                     LRFHGIDIPEEFTRYSVGNWSKRFLAWLREVLLSTEYGKEALGIHIEQFVRLRGILLG
                     QTRLLRALSRSDALKEPIRLLTSVPGIGITTAMSLAVEIDDIGRFCNADALASYIGLI
                     PMCHSSGESQANGNITVRKHAMLRCYIIEAAWIAIRKDPAMTMAYQGYRKRMNAQKAI
                     VKIARKLVNRIFFVLKHGQEYVPCVVN"
BASE COUNT          466 a          325 c          404 g          413 t
ORIGIN      
        1 aaaattagtt cctatgttta aacttggaaa agacggatag gtagtccagt aaaaaagcta
       61 tatcatttta gttaactttg tttttgagag ataacaatct taacttaaac aatatagctt
      121 atgaatacaa ggcaaagtaa caaattagat ttcaaaggac aaaatattta cataggaatt
      181 gatgtccatt tgaagagttg gtcggtatcg gtattgtcgg aacattcggt tctgaagagg
      241 ttcagtcaat ctccaagtcc ggaatcgctg cacaagtttt tgacaaccca ttatccggga
      301 gcggattatc attcggttta tgaagcgggc ttcagcgggt tctggataca tgagaagtta
      361 gtaacattgg ggatcaacaa tatcgtagtc aatccggcgg atgtgccgac catgagtaag
      421 gagaaattgc gcaagaccga cgcggttgat tgcgggaaac tggccaggga gttacggtcg
      481 ggaacattaa aaggtattta tgtgccggat atagccaccc tggaaatgcg ttctttgata
      541 aggttgagaa acaggatcgt aaaagacacc acgagggaga agaaccggat aaagtcattt
      601 ttacgcttcc acgggataga tatcccggag gaattcacac gttattcggt gggtaactgg
      661 tcaaagcggt ttctggcatg gctacgcgaa gtactccttt ctaccgaata tggaaaggaa
      721 gcactgggga tacatattga gcagtttgta cgcctgcggg gcatactcct ggggcaaaca
      781 cgcctgctgc gtgcgttatc ccgcagtgat gcactcaagg agccaatacg tctgctgact
      841 tcggtcccgg gaatagggat aaccaccgcc atgtcgttgg cggtcgagat agacgatatc
      901 gggcggtttt gcaatgccga tgcgctggcc tcctacatcg gtctgatacc gatgtgtcat
      961 tccagcgggg aaagtcaggc aaacggtaac atcacggttc gcaagcacgc catgttgcgc
     1021 tgttatataa tcgaggctgc ctggatagcc ataaggaaag acccggcaat gacgatggct
     1081 taccaagggt accgtaaacg gatgaacgct cagaaggcca tcgtcaagat tgcccgtaaa
     1141 ctggtgaaca ggatattttt cgtactcaaa catggacagg aatatgtgcc ttgtgttgtc
     1201 aattaacata caatcctaca gtgtaagcaa tttttccacg ggggaaacac tatcataatg
     1261 ttgcagcttt gaagtctgct taaattagta tgtgaccccc gcctatcgta aactgataca
     1321 ggaatttgca gctcccgccg aaaggacgga cagtctgctg tggaaagatt gtttaccgta
     1381 ggatgtagga tcgggttttc ttagccatat ggacagtatc agtaatacaa aaagggaggt
     1441 agaatctcga cctcccccag tagcattact gcgtaccgtc agggtgctta ttgctgacag
     1501 gttgcccccc ggcagagcct gtttcctctt aacaccctga cacaaagata aaatttaaaa
     1561 gttaattaaa gtgaattgtc caattttatt tggattttaa atggaaaa
//
LOCUS       DAOSDR010000497         1579 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrimonas sp. isolate AMR_MDS_1826
            NODE_26147_length_1579_cov_4.110892, whole genome shotgun sequence.
ACCESSION   DAOSDR010000497 DAOSDR010000000
VERSION     DAOSDR010000497.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863355
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrimonas sp. (wastewater metagenome)
  ORGANISM  Petrimonas sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Dysgonomonadaceae; Petrimonas.
REFERENCE   1  (bases 1 to 1579)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1579)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:10:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,720
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 35
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1579
                     /organism="Petrimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26147_length_1579_cov_4.110892"
                     /isolate="AMR_MDS_1826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2023866"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..329)
                     /locus_tag="PLI30_13670"
     CDS             complement(<1..329)
                     /locus_tag="PLI30_13670"
                     /inference="COORDINATES: protein motif:HMM:NF015506.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HOI80642.1"
                     /translation="MNSKTLLLSLSALYLITISAFASENSQLQPPPVYEGKIIENPDI
                     PPIYTGGPGEMNKFISGTLRYPSDAVERNVQGLVVYTFIVEKDGTLTNFDLIHRADSS
                     LDKEALR"
     gene            610..>1579
                     /locus_tag="PLI30_13675"
     CDS             610..>1579
                     /locus_tag="PLI30_13675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019538588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate synthase"
                     /protein_id="HOI80643.1"
                     /translation="MNLSTSPSVIKAHDISRFQFRDTPFVKLMNKRIYNVLFIASQYD
                     MFILEDDGRVDEQIFNEYTSLNLRYPPRFTQVSTAGQAMEELRANRYELIICMPNMDN
                     HEIFAQAKVIKHKFPYIPIVLLTPFSKSVTRVLEREDVSGMDYVFSWLGNADLLLAII
                     KLIEDSMNVESDVKTVGVQTIMLVEDSVRFYSSALPLLYKYVLNESKEFSKEALNDHL
                     RMMRMRGRPKILLARNYEEAVSLYKKYGDNMLGVISDISFSREGKKDKLAGRVLGEWI
                     RKKNKYIPIIYASSESENREYAALVDAKFIDKNSKTFPQDFHKAVKD"
BASE COUNT          443 a          314 c          366 g          456 t
ORIGIN      
        1 atacgtaagg cttccttgtc gagtgaagag tccgcacggt gaatcagatc gaaattggtc
       61 agtgtgccgt ctttttcaac tataaatgta taaacgacca atccttgcac atttcgctca
      121 acggcatcgg acgggtaacg aagagttccg gatatgaact tgttcatttc acccggtcct
      181 ccggtgtaaa tgggagggat gtccggattt tcgattattt ttccttcgta aacagggggc
      241 ggctgaagct gagaattttc ggatgcaaaa gcagaaatcg ttatcaggta taaagccgat
      301 aacgacaaaa gtaacgtttt agaattcatt tatttcttat tgatgtttag accttttttg
      361 tttgaagagc aaagataata aaaaaacgta acgtttttaa agcagcagtt tgagattaac
      421 aaataaattc atagatttgt ggcggttttt catcatcgtg gtaattctgt ttttttaatt
      481 ttccacgaaa aaaaagcagc cggctacgga agtgaagcgc cttttgccgg ctgctgcttt
      541 ttatggattc cctgtcaacc gtaaaccgca aaattttttt atctttgtcc tcttaagatt
      601 tcacccgata tgaacttatc cacttcacca tccgtaatta aagcgcatga tatcagtcgt
      661 tttcagtttc gagatacgcc ttttgtaaag ctgatgaaca agcgcattta caacgtccta
      721 tttatcgctt cacaatacga tatgtttatc cttgaagatg acggacgggt ggatgagcag
      781 attttcaacg aatatacgtc gcttaacctc cgctatccgc cgcgctttac gcaagtgagt
      841 acagccgggc aggctatgga agagcttcgg gccaaccgtt atgaactgat catttgcatg
      901 ccgaacatgg ataaccacga gatctttgcc caggcgaagg ttatcaagca caagtttcct
      961 tatatcccta ttgtgttgct tacccctttt tcaaaatcgg ttacgcgcgt gctcgagaga
     1021 gaagatgtct cgggaatgga ctatgttttc agctggctgg gaaatgctga tttgttgctg
     1081 gccatcatta aactgataga ggacagtatg aacgttgaaa gcgatgtgaa aacggttggt
     1141 gtgcagacca ttatgctggt ggaggattcc gtccgctttt attcgtcggc gttgcctctg
     1201 ctctacaaat atgtgctcaa cgagtcgaaa gagttctcca aggaggcact caacgatcac
     1261 ctgcgtatga tgcgtatgcg tggacgacca aaaatcttgc tggcgaggaa ttatgaggag
     1321 gcagtcagcc tttacaaaaa gtacggcgat aatatgttgg gtgttatctc ggatatcagc
     1381 ttcagccgtg agggtaaaaa agacaagttg gcgggaagag tgttgggtga atggatccgt
     1441 aagaagaata aatacatccc gatcatttac gcttcatcgg aatctgaaaa cagggaatac
     1501 gctgcactgg tggatgccaa attcatcgat aaaaattcaa agacctttcc tcaggatttt
     1561 cataaagctg taaaagata
//
LOCUS       DAOSDR010000498         1468 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrimonas sp. isolate AMR_MDS_1826
            NODE_28641_length_1468_cov_4.165605, whole genome shotgun sequence.
ACCESSION   DAOSDR010000498 DAOSDR010000000
VERSION     DAOSDR010000498.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863355
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrimonas sp. (wastewater metagenome)
  ORGANISM  Petrimonas sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Dysgonomonadaceae; Petrimonas.
REFERENCE   1  (bases 1 to 1468)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1468)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:10:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,720
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 35
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1468
                     /organism="Petrimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28641_length_1468_cov_4.165605"
                     /isolate="AMR_MDS_1826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2023866"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(14..>1468)
                     /gene="dxs"
                     /locus_tag="PLI30_13680"
     CDS             complement(14..>1468)
                     /gene="dxs"
                     /locus_tag="PLI30_13680"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008780643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HOI80644.1"
                     /translation="QPNNLLIILNDNEMSIDSNVGGIQDYLVKMTTSSKYNKMRNRVY
                     QSFKSKNLISEEKKNIVLRFNNSIKSLITQQQNMFEGFNIRYFGPVDGHDLPGLIRVL
                     HNIRDMKGPRLLHIKTVKGKGFKPAEKAATIWHAPGLFDKETGERIVRKRIDQPQLYQ
                     DVFGHTLVELAEENQKIVGITPAMPTGCSMTYMMQKLPDRAFDVGIAEAHAVTFSAGL
                     AKEGFIPFCNIYSSFMQRAYDQVIHDVALQKLKVIMCLDRAGLVGEDGPTHHGVFDLA
                     YLRPIPNLVISAPFNEHDLRNLMYTAVYGNDGPFVIRYPRGQGVMVDWRNPPQLIPIG
                     KGKRLKKGKDMALLSIGAIGNSAVKAIARAEEMGISVAHYDMLFLKPIDQEMLSKVAR
                     KFKYIITLENGVIKGGMGSAVIEFLADNGFIHNRIFRIGIADEFITHGAITELQKIAG
                     IDEEGILRKIMEVNELIKQEQAIVVIQEQSNLRN"
BASE COUNT          323 a          361 c          323 g          461 t
ORIGIN      
        1 acttttctgt tttctagttt ctcaggttac tttgttcctg aatgacgaca atggcctgtt
       61 cctgttttat taattcgttt acctccataa tttttcgtaa aataccctct tcatcaatcc
      121 ctgcaatttt ttgcaactct gttattgctc cgtgagtgat aaactcatct gctatcccga
      181 tcctgaaaat ccggttgtgg ataaaaccgt tatcagccag aaattctatt acggcacttc
      241 ccattccgcc ttttattact ccgttttcca gcgtgatgat gtacttgaat tttctggcaa
      301 ccttactcaa catctcctga tcgattggct tcaggaaaag catgtcgtag tgtgccacgc
      361 taattcccat ctcttctgcc cgtgcaatgg cttttaccgc gctgttacct atggcgccta
      421 tggaaagcag ggccatgtct ttgccctttt tcaacctttt cccttttcct attgggatga
      481 gctgcggtgg gttgcgccaa tccaccatta ccccctgccc acgcgggtac ctgatcacaa
      541 acggaccatc attgccgtaa acggctgtgt acattaggtt gcggagatcg tgttcattga
      601 aaggagccga aataaccagg ttgggaatgg gccgaagata tgccaggtca aataccccgt
      661 ggtgcgttgg cccgtcttcg cccaccagtc cggccctgtc gagacacatg atgactttga
      721 gcttttgcag cgccacgtcg tgaattacct gatcgtaagc ccgttgcata aacgatgagt
      781 agatattgca aaacgggatg aaaccctctt ttgccaatcc ggcggagaaa gtaaccgcat
      841 gcgcttcggc aattcccaca tcaaaagccc tgtccggcaa tttttgcatc atatacgtca
      901 tggagcatcc cgtgggcata gcgggtgtaa tcccgacaat tttctggttt tcttcggcaa
      961 gttccaccag tgtatgtccg aaaacatcct ggtagagttg cggttgatca attctttttc
     1021 ttacaatccg ttcgcctgtt tctttatcga ataacccggg agcatgccag atagtggcgg
     1081 ctttttctgc gggtttgaac ccttttccct tgacggtttt gatgtgaagc agtcttggtc
     1141 ccttcatgtc cctgatgtta tgcaaaaccc ggataagtcc cggcagatcg tgtccgtcaa
     1201 ctgggccgaa gtaccggata ttgaaccctt cgaacatatt ttgctgttgg gtaataaggg
     1261 attttatgct gttgttgaag cgaagcacaa tgtttttctt ctcttcggat ataaggtttt
     1321 tgcttttgaa ggattggtag accctgttac gcattttgtt gtacttggac gacgtggtca
     1381 ttttaaccag ataatcctga atacccccca cattactgtc gatagacatt tcgttgtcgt
     1441 tgagtatgat gagcaagtta ttcggctg
//
LOCUS       DAOSDR010000499         1466 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrimonas sp. isolate AMR_MDS_1826
            NODE_28679_length_1466_cov_5.792346, whole genome shotgun sequence.
ACCESSION   DAOSDR010000499 DAOSDR010000000
VERSION     DAOSDR010000499.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863355
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrimonas sp. (wastewater metagenome)
  ORGANISM  Petrimonas sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Dysgonomonadaceae; Petrimonas.
REFERENCE   1  (bases 1 to 1466)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1466)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:10:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,720
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 35
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1466
                     /organism="Petrimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28679_length_1466_cov_5.792346"
                     /isolate="AMR_MDS_1826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2023866"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(234..1442)
                     /locus_tag="PLI30_13685"
     CDS             complement(234..1442)
                     /locus_tag="PLI30_13685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI80645.1"
                     /translation="MAGNYFLKTLFGFLLKHRVLSIGTKYYPTNEKEQEYVEMVNYTR
                     TMLLEIDRAHITTENIFQNLEKEVGRGNIPDNRRFVEIKPAEDDVNEYALLSNIIMGS
                     DRYLYVEVFSGYKGIIDQFVQIIKDENGVIVERSNTEIVSRLLSKNDAIRVGIELIKI
                     GMEKEIDVRSAVGMTGAAAIERSINLNKQIGQTSGVGFTKLGGEFAIVFSSKLTNLEG
                     SPAVYDNYLFIDAFDSTQFIGEQGRDRLVEIMNEIKDFIEKECKGKIEGYREGGDDLI
                     ANLPTKDAALRAGIDSSWHALNNGARLRVGIGKSRREAGERAQMADDIKIWNNSPVMV
                     FDLADGVYAYYVPSEFTRTIVEYLQERSGRVIMIFVFVFIVTLVGWNIGHWEFGLVAI
                     GLALLYAVTA"
BASE COUNT          399 a          316 c          265 g          486 t
ORIGIN      
        1 tggtgttttt tatgttgtag attatgggaa gctcagggga ggaattatta ttgaagtaag
       61 aataatttcc atcattaaga tataatcctg agaatagact ggcaccagta ccgcaaccaa
      121 atgcaatgaa tgatatgatt agagcaactg aggcttttgc ttttaagtct tgtctcatct
      181 ttaatccctt cttaatataa taaatgggta aatttaattt attttatata tttttaagca
      241 gttactgcat aaagtaaagc taatcctatt gctaccaatc caaattccca gtgtccaatg
      301 ttccatccga ccaatgtgac tatgaagaca aagacaaaga tcatgataac tctaccgctt
      361 cgttcctgca agtattcaac tatggttcgg gtgaactcgg atggtacata gtaggcgtaa
      421 acaccgtccg ctaaatcgaa aaccataact ggggaattat tccagatttt gatatcatca
      481 gccatctgtg ctctttctcc tgcttcccgt cggctttttc caatacccac tcttagtctt
      541 gctccattgt tgagagcgtg ccaggatgaa tcgattcctg cacggagggc tgcatccttg
      601 gtggggagat ttgcaatgag atcatctccg ccctcacggt aaccttctat tttgcctttg
      661 cattcttttt ctatgaagtc tttgatctca ttcatgattt caaccagtct gtcccggccc
      721 tgttccccta tgaactgggt tgagtcaaag gcatcaatga aaaggtagtt atcgtaaact
      781 gccggtgatc cttccagatt ggtgagttta ctggagaaga caatggcaaa ttcaccacct
      841 aatttggtga aaccaacacc agatgtttgt cctatttgtt tgttgaggtt gatggatcgt
      901 tcaatggcag ctgcacctgt cattccaaca gcggatctca catcgatctc tttttccata
      961 ccaatcttaa ttaactcaat acctacacgt attgcatcat ttttggataa aagtcgggat
     1021 acgatttcgg tgttgcttct ttcaacaatc acaccgtttt catccttaat gatctgtacg
     1081 aattggtcga ttattccctt gtacccactg aaaacttcca catacaggta tcgatcacta
     1141 cccattatta tattgcttaa aagagcatat tcgttaacat catcttcagc aggttttatt
     1201 tcaacaaacc ggcgattatc aggaatatta cccctaccaa cctctttttc aaggttttgg
     1261 aaaatgtttt ccgtggtaat atgagctcgg tctatttcta aaagcatggt acgggtgtag
     1321 ttcaccattt ccacatattc ttgttctttt tcattggtag gatagtactt ggtacctata
     1381 ctcaaaaccc gatgttttaa aagaaagcca aatagcgttt tcaggaagta attgccagcc
     1441 atttatttgt ctcctccctt ttccaa
//
LOCUS       DAOSDR010000500         1445 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrimonas sp. isolate AMR_MDS_1826
            NODE_29206_length_1445_cov_3.969784, whole genome shotgun sequence.
ACCESSION   DAOSDR010000500 DAOSDR010000000
VERSION     DAOSDR010000500.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863355
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrimonas sp. (wastewater metagenome)
  ORGANISM  Petrimonas sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Dysgonomonadaceae; Petrimonas.
REFERENCE   1  (bases 1 to 1445)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1445)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:10:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,720
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 35
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1445
                     /organism="Petrimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29206_length_1445_cov_3.969784"
                     /isolate="AMR_MDS_1826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2023866"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1019)
                     /locus_tag="PLI30_13690"
     CDS             complement(<1..1019)
                     /locus_tag="PLI30_13690"
                     /inference="COORDINATES: protein motif:HMM:NF014851.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta galactosidase jelly roll domain-containing
                     protein"
                     /protein_id="HOI80646.1"
                     /translation="MRITQLLMVLFYLAVTFSAEAQTRVQLTLKKGWKFSREDNASAS
                     GINFNDASWQSVEVPHDWAIYGPFDRSNDIHRMAIVQDGQTKATEHYGRTGGLPFTGV
                     GWYRNRFSIPDFTGDKRVKIQFDGAMSNARVYVNGKEAGYWPNGYNTFYLDITGLINN
                     DGKENVLAVRLENFEEQSRWYPGAGLYRNVHLIVTDKTHIPIWGTYVTTPVVEKDVAR
                     VNVRTRVHASGEGNLKLVTEIRDKSFNTIASAENTLSKADMGEFSQNLAVENPQLWGI
                     KQPNLYTAVSRLYENNQLVDEYTTPFGIRTLEIKPNDGFYLNGEKIKFQGVCLHHDLG
                     PLGAAV"
     gene            complement(1067..>1445)
                     /gene="fumC"
                     /locus_tag="PLI30_13695"
     CDS             complement(1067..>1445)
                     /gene="fumC"
                     /locus_tag="PLI30_13695"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019539353.1"
                     /note="class II family (does not require metal);
                     tetrameric enzyme; fumarase C; reversibly converts
                     (S)-malate to fumarate and water; functions in the TCA
                     cycle; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HOI80647.1"
                     /translation="ILGNDVTIGVAGMQGHFELNVFKPVMAANFLQSARLLGDAAVSF
                     NEHCVSGIRPNLGRIKALVNNSLMLVTALNTKIGYYKAAEIAKAAHKNGTTLKEEAVR
                     LGYVTAEDFDEWVKPGNMTKPLD"
BASE COUNT          354 a          371 c          320 g          400 t
ORIGIN      
        1 actgcagccc ccagcggtcc caaatcgtga tgcaggcata caccctgaaa ttttatcttc
       61 tcgccattca ggtaaaaacc gtcgttgggc ttaatctcca gggtacgtat gccgaaaggc
      121 gtagtgtact cgtcaaccaa ttgattgttt tcgtagagcc tggacaccgc cgtatacagg
      181 ttcggctgtt taatccccca caattgggga ttttccacgg ccaggttttg tgaaaattca
      241 cccatgtcgg ctttagagag cgtattctct gcggaagcga ttgtattgaa cgacttgtcc
      301 cggatctcgg taaccagttt cagattgcct tctccggagg catgtacccg ggtgcggaca
      361 ttcacacgcg caacatcttt ttccacaaca ggcgttgtaa cgtaagtccc ccagatggga
      421 atgtgtgtct tgtccgtaac gatcaggtga acgtttcggt agagtcccgc accgggatac
      481 cagcgcgatt gttcttcaaa attttcgagg cggactgcaa gcacattctc ctttccatcg
      541 ttgtttataa gaccagtaat atcaagataa aacgtgttgt atccgttcgg ccaatatcca
      601 gcctctttcc cgttgacgta aactcgggca ttgctcattg ccccgtcgaa ctgaattttc
      661 acccgcttgt caccggtgaa atccggaatc gagaatctat tcctgtacca tccgacaccg
      721 gtgaaaggaa gtccgcccgt tcttccgtaa tgttcggtcg cttttgtctg accgtcctgg
      781 acaatagcca tccggtggat atcgttagac cggtcaaacg ggccgtaaat agcccaatcg
      841 tggggcacct ccaccgactg ccaagaggca tcgttgaaat ttattcccga ggcagatgcg
      901 ttgtcttcac gcgaaaactt ccagcctttt tttaacgtta attgtacacg ggtttgcgct
      961 tcagcagaaa aagtcactgc tagataaaac agaaccatca aaagttgagt tatcctcata
     1021 aaaagtaatt ttattgtatt gagttagtaa aattcagtaa aaatgttcaa tccagaggtt
     1081 tggtcatatt cccgggtttc acccattcat cgaagtcttc ggcggtaaca taacccaacc
     1141 gcaccgcctc ctcctttaag gtggttccgt ttttgtgagc agctttggct atctcggccg
     1201 ccttgtagta accgattttt gtattcaagg ccgttacaag catcaaggaa ttattcacca
     1261 atgccttgat tcttcctaag ttcggcctta ttcctgaaac gcaatgttca ttgaaagata
     1321 cggcggcatc tccaagaagg cgggcgctct gtaagaagtt cgccgccata accggtttaa
     1381 aaacatttaa ttcaaaatgg ccctgcatgc cggcgacacc tatcgtcaca tcgtttccca
     1441 ggatt
//
LOCUS       DAOSDR010000501         1444 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrimonas sp. isolate AMR_MDS_1826
            NODE_29249_length_1444_cov_2.096472, whole genome shotgun sequence.
ACCESSION   DAOSDR010000501 DAOSDR010000000
VERSION     DAOSDR010000501.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863355
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrimonas sp. (wastewater metagenome)
  ORGANISM  Petrimonas sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Dysgonomonadaceae; Petrimonas.
REFERENCE   1  (bases 1 to 1444)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1444)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:10:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,720
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 35
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1444
                     /organism="Petrimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29249_length_1444_cov_2.096472"
                     /isolate="AMR_MDS_1826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2023866"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..276)
                     /locus_tag="PLI30_13700"
     CDS             complement(<1..276)
                     /locus_tag="PLI30_13700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HOI80648.1"
                     /translation="MKIAVTGATGHLGTFVVEELKKRVSNENIVALVRTPGKASALEV
                     EVRAFDYNQPETMTAALAGVDRLVLISGNEIGQRARQHFNVIEAAGKA"
     gene            complement(305..871)
                     /locus_tag="PLI30_13705"
     CDS             complement(305..871)
                     /locus_tag="PLI30_13705"
                     /inference="COORDINATES: protein motif:HMM:NF016176.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HOI80649.1"
                     /translation="MKTINLLAILLLIGTLSASAQKSEIDLSKSQVLWTGKKIGGSHN
                     GKIQIQSGSFELKNGEVVSGNVVIDMNSLTNDDLKDAGYNKQLVDHLKSDDFFGVNNH
                     PTATFKITRAGKFNSDKALVTGLLTIKGKTEKVSFVVNRKDNVYSTQLKVDRSKFGVR
                     YGSKSFFNNLGDKVIDDMFTLDIKLVLK"
     gene            complement(1030..>1444)
                     /locus_tag="PLI30_13710"
     CDS             complement(1030..>1444)
                     /locus_tag="PLI30_13710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HOI80650.1"
                     /translation="TGCLRSFYIDSKGTEHIYQIRMDNSWISDLESFFTQTPSKYNIE
                     ALEDTQMLRISFQRLEQLYGEVPKLERYFRILFQKAYINALKRLNANMWETAMDRYND
                     MLKEHPMMFQRVPLIYIASYLGITPESLSRIRRRG"
BASE COUNT          335 a          330 c          305 g          474 t
ORIGIN      
        1 ggctttaccg gctgcctcta tcacattgaa atgctgacgg gcacgctggc caatttcgtt
       61 gcctgagata agtaccagcc ggtctactcc ggcaagtgct gctgtcatcg tttcgggttg
      121 gttgtaatca aaggctcgta cctctacttc cagggcagat gctttccccg gtgtgcggac
      181 aagtgccacg atgttttcat tggaaaccct cttttttaac tcttccacaa caaaggttcc
      241 taaatgcccg gttgctccag tgactgctat tttcatacga ttatttttat ttttatgtac
      301 gtttttattt cagaaccagt ttgatgtcca gcgtgaacat gtcgtcaatt actttgtcgc
      361 ccaagttgtt aaagaatgat ttggaaccgt atcgcacacc aaatttagag cggtctacct
      421 tcagttgtgt cgaataaaca ttgtcttttc tgtttaccac aaacgatacc ttctctgtct
      481 ttcccttgat ggtcagcaag cccgtgacca gtgctttatc cgaattgaat ttacccgctc
      541 tggttatttt aaacgtagcg gtggggtgat tgttcacacc aaaaaaatcg tccgatttca
      601 agtgatccac caactgcttg ttgtaacctg catctttcag gtcgtcgttg gtgaggctgt
      661 tcatgtcgat caccacattt ccgcttacaa cctcaccgtt ttttaactca aaagatccgc
      721 tctgtatctg aatttttcca ttgtgagagc cccctatttt tttcccggtc catagaacct
      781 gcgatttact caggtcgatt tcactttttt gtgccgatgc tgataaagtt cctatcaata
      841 aaaggattgc caataaatta attgttttca tacgtttttt agtttttttg tgggtgaaac
      901 tactcagata tgactgtccg tttcaaccgg tctgattttg aattgtttct ttaattatta
      961 cggcacaaag gtagcgtagt gccgtgcctc caacacttga tctatggtaa gaaatgaaat
     1021 tgatttgtgt taacctctcc tcctgatgcg gctcaaactt tccggggtaa tgcccaggta
     1081 ggaggcaatg tagattaagg gaacccgctg aaacatcatg ggatgttctt tcagcatgtc
     1141 gttgtaacga tccatggccg tttcccacat gtttgcattg agtcgtttca gtgcgttgat
     1201 gtacgccttc tgaaaaagga tgcgaaagta tctctccagc ttgggaactt ctccgtataa
     1261 ctgttccagt cgttgaaagg aaatacgcag catctgtgtg tcctccaatg cctcgatgtt
     1321 gtacttggaa ggtgtttgcg tgaagaaact ctccaggtca ctgatccagc tattgtccat
     1381 cctgatctgg tagatgtgtt ctgtcccctt tgaatcgata taaaaagagc gcaagcagcc
     1441 tgtg
//
LOCUS       DAOSDR010000502         1368 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrimonas sp. isolate AMR_MDS_1826
            NODE_31321_length_1368_cov_4.976390, whole genome shotgun sequence.
ACCESSION   DAOSDR010000502 DAOSDR010000000
VERSION     DAOSDR010000502.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863355
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrimonas sp. (wastewater metagenome)
  ORGANISM  Petrimonas sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Dysgonomonadaceae; Petrimonas.
REFERENCE   1  (bases 1 to 1368)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1368)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:10:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,720
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 35
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1368
                     /organism="Petrimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31321_length_1368_cov_4.976390"
                     /isolate="AMR_MDS_1826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2023866"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..216
                     /locus_tag="PLI30_13715"
     CDS             <1..216
                     /locus_tag="PLI30_13715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009125911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase C-terminal beta-propeller
                     domain-containing protein"
                     /protein_id="HOI80651.1"
                     /translation="NKSGITIRVKISDIRTMGRATQGVRLINLEKRNDEIASVCKVNS
                     EEEIDEVETVLPEDVDSEENFTDEVSE"
     gene            319..>1368
                     /locus_tag="PLI30_13720"
     CDS             319..>1368
                     /locus_tag="PLI30_13720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019540828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOI80652.1"
                     /translation="MRKLFFLTAVAVFAASSMFAQKSALRDAKRSLGSNDLNEARTLI
                     KQATTNAETANDPETWKIFGDIGNKAFDNERTNEMLGKQANQEVMYNGLLESYTPYLK
                     ADSLGELPDEKGKVKNKFRKDIAGVLKANHPFYINGGIFFNEKQQYAKASDFFEIYWN
                     IPSLPMFAGQKDPFLIDSTFQTIKYYAIITAIQAQDHKRALAMLKRAASEPFIANSAY
                     QESDIYELIASEYIQQGDTAKYIETLYEGAEKFPKSKYFTPNLVNVFIRQGDNQKAME
                     YLDEAIKNDPSNACDLNSVKGALLAEKGDFAAAEEEYNKALTQDPNCERALEALAVNF
                     ILQAQNLKEKTATMSD"
BASE COUNT          491 a          260 c          285 g          332 t
ORIGIN      
        1 aacaaatcgg gcataacaat acgtgtgaaa atttccgata ttcgcacaat gggacgtgct
       61 acacagggag ttcggctcat aaacctggaa aaacgaaacg atgaaatcgc ttcggtatgt
      121 aaagttaatt ccgaagaaga aatagatgag gtggaaaccg ttttgccgga agatgtcgat
      181 agtgaggaaa attttacgga tgaggtcagc gaataatgta catattttaa actttttgta
      241 tttttacagt caaataaatc aatagaaatt ttaaacaatt aagaaaagac gaatacttaa
      301 cttaaaacga tgaataaaat gagaaaatta tttttcctta ctgcggtggc tgtctttgct
      361 gcaagttcca tgtttgcaca aaagtcggct ctgagagacg ccaagagatc tttaggaagc
      421 aacgacctga atgaagctcg caccctgata aaacaggcca ctactaacgc agaaaccgcc
      481 aatgatcctg aaacctggaa aatctttgga gatatcggta acaaggcatt cgacaacgag
      541 agaaccaatg aaatgttggg aaaacaagca aaccaagaag tgatgtacaa cggactgttg
      601 gaatcttaca caccgtatct gaaagccgat tctctaggtg agttgccgga tgaaaaaggc
      661 aaagtgaaaa acaaattcag aaaagacatc gccggagttt taaaagctaa tcatcctttc
      721 tacatcaacg ggggaatttt ctttaacgaa aaacaacaat atgccaaagc ttccgatttc
      781 ttcgaaatat actggaacat tccttcgtta ccgatgtttg ccggacagaa agaccctttt
      841 ctgatcgaca gtacttttca aaccattaaa tattatgcca ttatcacggc tattcaggcg
      901 caagaccaca aaagagcatt agctatgctg aaaagagcgg ccagtgaacc ttttatcgcc
      961 aacagtgctt atcaggaaag cgatatttac gaacttattg ccagcgaata tattcaacag
     1021 ggagatactg ctaaatatat agaaactctt tacgaaggag cggaaaaatt ccccaaaagc
     1081 aagtatttta ctcctaatct ggtaaacgtt tttatccgtc agggcgacaa tcagaaagcc
     1141 atggaatacc tagatgaagc cattaagaac gatccttcca acgcgtgtga tttaaacagt
     1201 gtaaaagggg cattattggc agaaaaagga gattttgcag cagctgaaga agaatataac
     1261 aaggcattaa cgcaagatcc caattgtgaa agagcgcttg aagcacttgc ggtgaacttt
     1321 atcttgcagg ctcagaatct gaaggaaaaa acagcaacca tgtcggac
//
LOCUS       DAOSDR010000503         1346 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrimonas sp. isolate AMR_MDS_1826
            NODE_31976_length_1346_cov_2.331526, whole genome shotgun sequence.
ACCESSION   DAOSDR010000503 DAOSDR010000000
VERSION     DAOSDR010000503.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863355
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrimonas sp. (wastewater metagenome)
  ORGANISM  Petrimonas sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Dysgonomonadaceae; Petrimonas.
REFERENCE   1  (bases 1 to 1346)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1346)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:10:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,720
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 35
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1346
                     /organism="Petrimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31976_length_1346_cov_2.331526"
                     /isolate="AMR_MDS_1826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2023866"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1346
                     /locus_tag="PLI30_13725"
     CDS             <1..>1346
                     /locus_tag="PLI30_13725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019541223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOI80653.1"
                     /translation="TKTASKGRTSLNYTGNLSSKRPTTVPDNIVDSYPWAKSFSDAWS
                     NWNDDGRTPTAVNKTLSFSPEYLAEIKRRWEDPSLPRIEINPTTGAYEYYYSTDWYKE
                     LYKDSFFAQDHNVSVSGGNELASFYVSGRYNSEDGLYKYNTDTYSMYNLRARGNIQIF
                     DWLLVDNNTEYSTMKYHQPINVGEGSNIWRNMADEGHPLAPLTNPDGTLTFPAAYTIG
                     DRYLGKSGADLHQKIIKNKTALKADFFDKSLTLQADFTFQNTEYGHRQIRVQVPYSRY
                     EGQIGYTGTNTNDLQERRSTTEYLATNVYANYLKSFNKKHNFEFLAGFNYEQSTYNNV
                     TMTRNGIVYEDAKDINLALGNNINTSGGYSRWRIAGNFFRVNYNYLQRYLIEINGRYD
                     GSSRFPSNQQWAFFPSFSAGWRLSEEAFWQVNPDVISNIKFRVSYGSLGNGSINPY"
BASE COUNT          445 a          275 c          275 g          351 t
ORIGIN      
        1 caactaaaac tgcgtcaaaa ggaagaacat cattgaatta caccggaaat ttgtcttcca
       61 aacgtcctac aactgtcccc gataatatcg tggattcata cccgtgggca aaatctttta
      121 gtgatgcatg gtccaattgg aatgatgacg gaagaacacc caccgccgtc aataagacgc
      181 tctctttctc tccagaatac ttggccgaga taaaaaggag atgggaagac ccctccttac
      241 cgcgcataga gataaatcca accaccggag cttatgaata ttattacagc accgattggt
      301 ataaggaact gtataaggat agtttttttg cacaggatca caatgtatcc gtttcaggag
      361 gaaatgaact ggcttctttt tatgtaagtg ggaggtataa cagtgaggat ggactctata
      421 aatacaacac ggatacctat tctatgtaca atcttcgtgc cagaggcaac atccaaatat
      481 tcgactggtt actggtcgat aataatacag agtattcaac aatgaaatac catcaaccga
      541 taaatgtggg tgaagggagc aacatctggc gcaatatggc tgatgaaggc catcctttag
      601 ccccgctcac aaatcccgat ggaaccttga cgtttccggc tgcatatacc attggagaca
      661 ggtatttagg gaaaagtgga gccgacttac atcaaaaaat tattaaaaac aaaaccgcat
      721 taaaagcaga cttttttgac aaaagtttaa cattgcaggc cgattttact tttcaaaata
      781 cggaatacgg tcatcggcaa atacgggtac aagtgcctta tagccgttac gaagggcaga
      841 tcggttatac cggcacaaac acgaatgact tacaagaacg tcgctctaca acagaatatc
      901 tggcaacaaa tgtgtatgcc aattatttga agtcattcaa caaaaaacat aatttcgaat
      961 tcctggcagg attcaactac gaacaatcta cttataataa tgtaacaatg acaagaaacg
     1021 gtattgttta cgaagacgca aaagatatta atcttgcttt gggaaataac atcaacacat
     1081 caggcggata tagcaggtgg cggattgcag gaaatttctt ccgtgtaaac tacaattacc
     1141 tgcaaaggta cctgattgag ataaacgggc gttatgacgg ttcatcaaga tttccaagca
     1201 accaacagtg ggcattcttt ccttcatttt ccgcaggttg gcgattatct gaagaggcct
     1261 tctggcaggt taatcccgat gttatttcaa acatcaagtt tagagtgtca tacggatctt
     1321 tgggtaatgg atccattaat ccatat
//
LOCUS       DAOSDR010000504         1285 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrimonas sp. isolate AMR_MDS_1826
            NODE_33871_length_1285_cov_3.889431, whole genome shotgun sequence.
ACCESSION   DAOSDR010000504 DAOSDR010000000
VERSION     DAOSDR010000504.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863355
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrimonas sp. (wastewater metagenome)
  ORGANISM  Petrimonas sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Dysgonomonadaceae; Petrimonas.
REFERENCE   1  (bases 1 to 1285)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1285)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:10:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,720
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 35
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1285
                     /organism="Petrimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33871_length_1285_cov_3.889431"
                     /isolate="AMR_MDS_1826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2023866"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1216)
                     /locus_tag="PLI30_13730"
     CDS             complement(<1..1216)
                     /locus_tag="PLI30_13730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005639561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylsulfatase"
                     /protein_id="HOI80654.1"
                     /translation="MKQLKSLLATGALSLGGIVFAQPKPNVVFIIADDLGYGDLSCFG
                     QEKFLTPNIDRLALNGTRFTRSYSGTTVSAPSRASLMTGLHTGHASIRGNKEMEPEGQ
                     FPLPENTYTLFHLFKNAGYKTGAFGKWGLGYPGSTGDPIHQGVDEFFGYNCQRLAHNY
                     YPGHLWRNQKRIELPENDNGRSGAYAQDLIQKEILSFISNNKGNPFFLYAPIVLPHAE
                     LIVPEDSIIQQFRGKFPETPYRGIDDGPSFRKGGYASQEHPRATHAAMVKRIDVYVGQ
                     IIERLKAEGIYENTLIVFTSDNGPHREGGGDPDFFNSNGIYRGYKRDLYEGGIRVPTV
                     VSWKGHVAAGEENSFAFAFWDYMPTFAELLGVEYPKPTDGVSVLPTFLNRDGQKSRDF
                     FYFEFLEMDGKQA"
BASE COUNT          337 a          320 c          278 g          350 t
ORIGIN      
        1 cggcctgctt tccatccatt tccagaaact cgaaatagaa gaaatcgcgg cttttctgtc
       61 cgtctctatt cagaaacgta ggaagaacac tcaccccatc agtaggtttt gggtattcca
      121 cacccaacag ttcggcaaaa gtgggcatgt aatcccaaaa agcgaaggca aagctgtttt
      181 cttctccggc cgccacatgg cctttccacg aaacaacagt cggcacacgg attcctcctt
      241 cgtacagatc gcgtttgtat ccgcggtaga tgccgttgct gttgaaaaaa tcgggatctc
      301 ccccaccttc gcgatgtggg ccgttatcac tcgtaaaaac gatcaatgta ttttcgtaaa
      361 taccttcggc tttgagtctt tcaataatct gccccacata cacatcaatt cgctttacca
      421 tagccgcgtg agtagcacgg ggatgttcct gtgaagcata tccccctttc cggaacgaag
      481 gaccatcatc tatcccccta taaggtgttt ccggaaattt tccacgaaac tgttgaataa
      541 tggagtcttc gggaacgatc agttccgcat gtggaagcac tatgggagca taaagaaaga
      601 acgggttgcc tttattgtta ctgataaaac tcagtatttc tttttgtatg aggtcttgcg
      661 cataagctcc ggatcgtccg ttatcgtttt cgggcaactc tattcttttt tgattgcgcc
      721 acagatggcc gggatagtag ttgtgggcca atcgctgaca gttataaccg aaaaactcat
      781 ccactccctg atggattgga tcaccggttg atccgggata tcccagtccc cattttccaa
      841 aagcgccggt tttgtagccg gcatttttaa acagatgaaa aagtgtatac gtgttttccg
      901 gtaacggaaa ttgaccctcc ggctccatct ctttgtttcc acggatagac gcatgaccgg
      961 tatgcaatcc ggtcatcagg ctagcacgcg agggagcact tacggttgta ccggaatagc
     1021 tccgcgtaaa ccgagttccg ttaagcgcca gccggtcaat gttcggtgtc aggaatttct
     1081 cctgcccgaa acagcttaaa tcaccgtaac cgaggtcgtc ggcaataata aaaactacgt
     1141 tgggttttgg ttgagcaaaa acaattcctc ccaaagaaag ggctcctgtt gccaacaacg
     1201 attttagttg tttcataaga gatcgatcct gttaaacctt caaaaaaact ttctttttat
     1261 aaagtatgta taagaatacc caacc
//
LOCUS       DAOSDR010000505         1253 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrimonas sp. isolate AMR_MDS_1826
            NODE_34896_length_1253_cov_3.969115, whole genome shotgun sequence.
ACCESSION   DAOSDR010000505 DAOSDR010000000
VERSION     DAOSDR010000505.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863355
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrimonas sp. (wastewater metagenome)
  ORGANISM  Petrimonas sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Dysgonomonadaceae; Petrimonas.
REFERENCE   1  (bases 1 to 1253)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1253)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:10:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,720
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 35
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1253
                     /organism="Petrimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34896_length_1253_cov_3.969115"
                     /isolate="AMR_MDS_1826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2023866"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..524
                     /locus_tag="PLI30_13735"
     CDS             <1..524
                     /locus_tag="PLI30_13735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005863410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="His/Gly/Thr/Pro-type tRNA ligase C-terminal
                     domain-containing protein"
                     /protein_id="HOI80655.1"
                     /translation="DLSQHEKYSGKKMQYFDPELNSSYVPYVVETSIGVDRMFLSVLT
                     SSYCEEKLEGGEIRVVLKIPAAIAPVKLAVLPLVKKDGLPEKATEIMDALKFSFACQY
                     DEKDSIGRRYRRQDAIGTPYCVTVDHQTLKDNTVTVRDRDTMEQKRVTINEIEKEISF
                     NTDIKQLLKKLNK"
     gene            591..1139
                     /locus_tag="PLI30_13740"
     CDS             591..1139
                     /locus_tag="PLI30_13740"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012478.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HOI80656.1"
                     /translation="MKTTLYSFVVACLSLFLVSCEKTVSFDDEFKMDNEKQFAKITAS
                     SEYTKIESQSGNGHIMYKEITDGDTGETPYFTDQVSVLYTGWFKRYWTKEDTFTGDDG
                     NLFKNKVIFDSTADRNNVPSKFTVGSELIDGFSTALQHMEVGDRWEVWIPWQLGYGAT
                     GRGDIKGYSTLVFEIELLEIVK"
BASE COUNT          397 a          257 c          281 g          318 t
ORIGIN      
        1 ttgatttgtc ccaacacgaa aaatactcgg gcaagaagat gcagtatttc gatccggaac
       61 tcaacagttc ctacgttcct tacgtagtgg aaacttctat cggggtagat cggatgtttc
      121 tttcagtcct caccagctcc tattgcgaag agaaactgga aggcggggaa attcgcgtag
      181 tgctgaaaat tcctgcagcc atagctcccg tgaaactagc tgtccttccc ttggtaaaaa
      241 aagacggatt gcccgaaaaa gcaactgaaa taatggatgc attaaaattc agttttgctt
      301 gtcagtacga cgaaaaagac agcattggca ggcgctatcg ccgccaggat gctattggca
      361 cgccttactg cgtcaccgtt gaccaccaga cattaaaaga caacaccgtg acggttcgtg
      421 accgggacac gatggagcaa aaacgggtta ccatcaacga aattgagaaa gaaatttcat
      481 tcaacaccga cataaaacag ttgcttaaaa aacttaataa ataaaaagac atcagaagtc
      541 agaacacaat ctttgcaatt gggtcctggt tctaaaaatc tttaaagaat atgaaaacaa
      601 cattatactc cttcgtcgtt gcctgcttgt ccctgtttct tgtttcctgc gagaagacag
      661 tcagttttga tgatgagttc aagatggata acgagaagca atttgcgaaa attactgcaa
      721 gctcagaata cacaaaaatt gaatcccaat cgggaaacgg acacatcatg tacaaagaga
      781 tcaccgacgg tgataccggg gaaacgcctt attttacgga tcaagtcagt gtgttgtaca
      841 ccggttggtt caaaaggtat tggaccaagg aggatacatt taccggcgat gacgggaatc
      901 tttttaagaa caaagtgatt tttgactcaa ccgcagacag gaataatgtt cccagtaaat
      961 ttaccgtggg cagcgaatta attgacggat tcagcactgc cttacaacac atggaggtag
     1021 gagatagatg ggaggtatgg attccctggc aattgggtta tggagccaca ggtagaggtg
     1081 atataaaagg ctactccacc ctggttttcg aaattgagtt gttggaaatt gtgaaataaa
     1141 aacctccata gtactacaac gtcgataaaa atttctcaat ggcactcctc acttgtgggc
     1201 gggtgccatt gaatttatta atcatcaccg tgaaggcata ttgcttgttt cca
//
LOCUS       DAOSDR010000506         1206 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrimonas sp. isolate AMR_MDS_1826
            NODE_36568_length_1206_cov_3.050391, whole genome shotgun sequence.
ACCESSION   DAOSDR010000506 DAOSDR010000000
VERSION     DAOSDR010000506.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863355
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrimonas sp. (wastewater metagenome)
  ORGANISM  Petrimonas sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Dysgonomonadaceae; Petrimonas.
REFERENCE   1  (bases 1 to 1206)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1206)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:10:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,720
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 35
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1206
                     /organism="Petrimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36568_length_1206_cov_3.050391"
                     /isolate="AMR_MDS_1826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2023866"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1147)
                     /locus_tag="PLI30_13745"
     CDS             complement(<1..1147)
                     /locus_tag="PLI30_13745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019540928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HOI80657.1"
                     /translation="MNLPKVNAHLHTPFSFSAFRDIDDALDRAVAEKVKVVGINDFYT
                     AEGYEEWMEGCSKRGLYPLFGIEFISLNEEDQKAGLRVNDPGNPGRTYISGKGLLQPF
                     KLSEPYASQLTNVRTEANKQVEAMCEKLNGILTAKNVGFVLDMDWIKDNLSKGSVRER
                     HLAKALRLKVYENSGDNPTSIKNLLEEIFDGKELKSDTENLAGVENEIRANLLKAGGS
                     AFVPETVEAFLPMQTVCKIILAAGGIPTYPFLADDAQGNYTDFERDLEHVAKQLTERG
                     FHSVEFITTRNDMNLLEKYATYLHNQGFIVTLGSEHNSPSMEPIELFARNQTPLSEKL
                     LRVNYEGACIIAAHQHLVAQGLSGYLDEQGNADRSKRGEFVKWGDELI"
BASE COUNT          306 a          310 c          238 g          352 t
ORIGIN      
        1 tgattaactc atctccccat ttcacaaact cacctctttt tgagcggtcg gcgttcccct
       61 gttcatccaa ataaccgctc agtccctggg caaccaaatg ttgatgagct gcaataatac
      121 acgcaccctc atagttgacc cgcagtaatt tttccgataa cggggtctgg ttgcgggcaa
      181 aaagttcaat aggttccatt gacggagagt tgtgttcact gcccagggtt acgataaaac
      241 cctggttgtg aagatatgtt gcatatttct ccaacaggtt catatcgttt cgggtggtaa
      301 taaattccac ggaatgaaac ccacgctcgg tcagttgttt tgccacgtgt tccaggtcac
      361 gttcaaaatc ggtataattt ccctgggcat catccgctaa aaagggataa gtaggtattc
      421 ctccggctgc cagaatgatt ttgcataccg tctgcatcgg aagaaacgcc tcgacagtct
      481 cgggaacaaa agccgaacca cctgctttca acaggttagc acggatctcg ttctctacac
      541 ctgcaagatt ttcagtatcc gacttcagtt cttttccatc gaaaatttct tctagcaggt
      601 tctttatgct tgttgggtta tcgcctgaat tttcataaac tttcaagcgt aaagccttcg
      661 ccaaatgacg ttccctaaca gaacccttac tcagattatc cttaatccaa tccatatcga
      721 gtacaaatcc gacattttta gcagtaagga ttccgttcag tttttcacac atggcttcca
      781 cctgcttatt agcctctgta cgtacattcg tcaattgcga agcataaggc tcactcagct
      841 taaagggctg taacaatcct ttcccgctga tgtaagtccg tcctggattt ccgggatcgt
      901 tcacccgcaa acctgctttt tgatcctctt cgttcaggct gataaactcg atgccgaaaa
      961 gcggataaag tccacgtttg gaacaacctt ccatccactc ttcgtaacct tcggcagtat
     1021 aaaaatcgtt gataccaacc actttaacct tttcagcaac ggccctgtcc agcgcatcgt
     1081 caatatcgcg gaaagcactg aaagagaacg gggtatgcag gtgggcattt acttttggta
     1141 aattcatgat caaattcctt tttttgctct cctgatcatt tcggcatcgg tgaaattctc
     1201 accggg
//
LOCUS       DAOSDR010000507         1189 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrimonas sp. isolate AMR_MDS_1826
            NODE_37233_length_1189_cov_4.192240, whole genome shotgun sequence.
ACCESSION   DAOSDR010000507 DAOSDR010000000
VERSION     DAOSDR010000507.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863355
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrimonas sp. (wastewater metagenome)
  ORGANISM  Petrimonas sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Dysgonomonadaceae; Petrimonas.
REFERENCE   1  (bases 1 to 1189)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1189)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:10:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,720
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 35
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1189
                     /organism="Petrimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37233_length_1189_cov_4.192240"
                     /isolate="AMR_MDS_1826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2023866"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            20..613
                     /locus_tag="PLI30_13750"
     CDS             20..613
                     /locus_tag="PLI30_13750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019539542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4290 domain-containing protein"
                     /protein_id="HOI80658.1"
                     /translation="MIYNTQKKKLIMPEYGRNIQNMVDHCVMLKDKDERRKCAYAVVD
                     IMGSMFPHLRDVNDFKHILWDHLAIMSDFKLDIDYPYEVVTKEDLNTAPDKLNYSRPT
                     MKFRHYGKLLEKMITIAADMEDGEKKDLLIAQLTTQMKKSYSQWNKEVDDEKIIADLL
                     ELSGGKIQLDNESYSIADIKSTASKRRLKTIKSQRRK"
     gene            617..1150
                     /gene="rimM"
                     /locus_tag="PLI30_13755"
     CDS             617..1150
                     /gene="rimM"
                     /locus_tag="PLI30_13755"
                     /inference="COORDINATES: protein motif:HMM:TIGR02273.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HOI80659.1"
                     /translation="MITKDEVFKIGKLQKPYGIKGEISLVFDKPAYTGIDAEFYFLDI
                     DRIFVPFLIEEITFITDTGARVKFEDVNDETGAARFANLHVFLLRKQVPENLDEENPE
                     WDFFIGYRVVDQHDRNLGTIESVDAATLNVLFIVKKANEEYLIPATDDFITRINEEQK
                     IICMNLPEGLVDTEGNL"
BASE COUNT          384 a          225 c          274 g          306 t
ORIGIN      
        1 atcaaaaaat aaataaaata tgatatacaa cactcagaag aaaaaactga ttatgcccga
       61 atacgggaga aacatacaga atatggtgga tcactgcgta atgcttaagg acaaggatga
      121 gcggaggaaa tgtgcttatg ccgtagtcga tattatggga agtatgttcc cgcatttgcg
      181 tgatgtgaat gattttaagc atattttgtg ggaccatctg gccataatgt ccgactttaa
      241 gcttgatatc gactatccct atgaagtggt aacaaaagag gacttgaaca cagcacctga
      301 caaattaaac tacagccgcc ctacaatgaa attcaggcat tacgggaaat tattggaaaa
      361 gatgattacc attgctgccg atatggagga tggggagaag aaggatttgc ttattgccca
      421 gttgaccacc caaatgaaaa aatcatattc ccaatggaat aaagaggttg acgatgagaa
      481 aataattgct gacctgcttg agctttcggg tggaaaaatt cagctggaca atgaaagtta
      541 ctccattgca gatattaaaa gcacggcttc caagcgcagg ttgaaaacaa ttaagtcgca
      601 gcggcgtaag taacgaatga taaccaagga tgaggtcttc aaaataggga agctacaaaa
      661 accttacgga ataaaaggtg aaatctcact cgttttcgat aaaccggcgt acaccggtat
      721 cgatgccgaa ttttatttcc tggatattga ccgtattttt gttccttttc ttatcgagga
      781 aatcacgttt ataaccgaca caggcgcccg ggtaaaattt gaggacgtga acgatgaaac
      841 cggcgctgcc aggtttgcta acctgcacgt tttcttgctt cgcaaacagg tgcctgagaa
      901 tctggatgaa gagaacccgg agtgggactt ttttatcggt taccgggttg tcgaccagca
      961 tgaccgtaat ctcggtacca tagaaagcgt ggatgcggca accctgaatg ttctttttat
     1021 tgtgaagaaa gcaaacgaag aataccttat ccccgccacg gacgatttta tcacccggat
     1081 aaacgaagaa caaaagatta tctgtatgaa tctacccgaa ggattggtag atacggaggg
     1141 taacctgtaa actccaaatt gattgtaggg atcctcgttt ttttttatc
//
LOCUS       DAOSDR010000508         1158 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrimonas sp. isolate AMR_MDS_1826
            NODE_38561_length_1158_cov_1.439710, whole genome shotgun sequence.
ACCESSION   DAOSDR010000508 DAOSDR010000000
VERSION     DAOSDR010000508.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863355
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrimonas sp. (wastewater metagenome)
  ORGANISM  Petrimonas sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Dysgonomonadaceae; Petrimonas.
REFERENCE   1  (bases 1 to 1158)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1158)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:10:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,720
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 35
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1158
                     /organism="Petrimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38561_length_1158_cov_1.439710"
                     /isolate="AMR_MDS_1826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2023866"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(183..374)
                     /locus_tag="PLI30_13760"
     CDS             complement(183..374)
                     /locus_tag="PLI30_13760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI80660.1"
                     /translation="MVENGIYRQKVSVFNEVKSANITINPDKEYRFIVKLLGFLTAEA
                     REEGFTDRVFSEGAVIEIK"
     gene            complement(387..593)
                     /locus_tag="PLI30_13765"
     CDS             complement(387..593)
                     /locus_tag="PLI30_13765"
                     /inference="COORDINATES: protein motif:HMM:NF027627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4945 domain-containing protein"
                     /protein_id="HOI80661.1"
                     /translation="MKKIEKLIIAALTLILFTGCYDRDIIDRKDFNHSLSKVENLSYT
                     QQGNVITLSWQIPDNIPEDFRRPL"
     gene            complement(689..>1158)
                     /locus_tag="PLI30_13770"
     CDS             complement(689..>1158)
                     /locus_tag="PLI30_13770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005802079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HOI80662.1"
                     /translation="SMNKRMIRYDEVLLWKAEVLIQLDRWNEALAPINKVRERAANSA
                     DWLIKADGTSVMNYHVEIYKPGENCVWDKDFAWKAMQWENRLEMAGEGRRFFDLQRWG
                     NLEEIMNNYFTVEKTRFSWMANARFTAGRDEFFPIPQNQMKWAKGNYTQNPGY"
BASE COUNT          328 a          276 c          189 g          365 t
ORIGIN      
        1 aatacaatca tcgtaacaat aacaaaggat atacatataa aataattttt tctttccatc
       61 taaattctac gcacaatttt cagcaaacaa ggattaaaag ggtgtaccgc caggaagcat
      121 cattaacggt atgcacctgt ccgtggtaca cccttatcga ataaattaca acttaccgat
      181 catcatttaa tttcaattac agctccttct gaaaaaaccc tgtccgtaaa cccttcctct
      241 cttgcttcgg cagtcagaaa gcccagcaat ttaactataa acctgtactc tttatccgga
      301 tttattgtaa tatttgctga cttaacttca ttgaatacgg aaactttttg cctgtagatg
      361 ccattttcaa ccacctgaat acttgtctac aaaggccttc tgaaatcttc aggaatattg
      421 tccggtatct gccacgacag cgtaatcaca ttcccctgtt gagtgtatga taaattttcc
      481 actttggaca aagaatgatt gaaatctttc ctgtcgataa tgtctctatc gtagcacccg
      541 gtaaacagga tcaaggtaag tgctgcaata attaattttt ctattttttt catacgatat
      601 tgttttacta ttttacacta aggttgtaca gaactcacct attatcgcgc ttgttctaca
      661 accataacat aggttcttac ccggaggatt agtatccggg gttttgtgta tagttacctt
      721 tagcccactt catctgattt tgcgggatag ggaaaaattc atcacgtccg gcagtaaacc
      781 gggcgtttgc catccatgag aaccgggttt tctccacggt aaaatagttg ttcataatct
      841 cttccagatt tccccaacgc tgcaaatcga agaaacgacg tccttcaccg gccatttcca
      901 aacggttttc ccactgcatt gctttccagg caaaatcttt atcccagaca caattctcac
      961 ccggcttgta aatctcaaca tgatagttca ttaccgatgt tccgtctgct ttgattaacc
     1021 aatcggcact attagcagct cgttcacgaa ctttgttgat gggtgctaac gcttcgttcc
     1081 acctgtccaa ctgaataagt acttcggctt tccagagcaa gacctcatcg taacgaatca
     1141 tccgcttgtt catagaat
//
LOCUS       DAOSDR010000509         1083 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Petrimonas sp. isolate AMR_MDS_1826
            NODE_41843_length_1083_cov_2.879377, whole genome shotgun sequence.
ACCESSION   DAOSDR010000509 DAOSDR010000000
VERSION     DAOSDR010000509.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863355
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Petrimonas sp. (wastewater metagenome)
  ORGANISM  Petrimonas sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Dysgonomonadaceae; Petrimonas.
REFERENCE   1  (bases 1 to 1083)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1083)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:10:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,720
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 35
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1083
                     /organism="Petrimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41843_length_1083_cov_2.879377"
                     /isolate="AMR_MDS_1826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2023866"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..890
                     /locus_tag="PLI30_13775"
     CDS             <1..890
                     /locus_tag="PLI30_13775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019539775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SusD/RagB family nutrient-binding outer membrane
                     lipoprotein"
                     /protein_id="HOI80663.1"
                     /translation="TYIDQAVASGVFTSNEDEAMFKYSKEAPNECYFYRGYFVDGRND
                     FSITKPFTDLLKGQPDTLNNKQHPWEGVVDPRLSIYTTPRGGKYLGLPYGLPSNNMTR
                     SIRDAAPSWYPASPPIILNKDFSVPLMTYAELCFILSERYNWDQEWYEEGVRSSVEHW
                     YALAGNAVDQTTLDSYVESVSETINAETVATQKYIHLYMQGTEAWSEYRRTGYPLTML
                     KPGEFTYVVGGEKVAFTPLSDTKGDLPARVKYPTNESTLNPDGFNAAIAKLQDGTNNY
                     YSKMYWDVRSEQNPHPTNK"
BASE COUNT          347 a          235 c          221 g          280 t
ORIGIN      
        1 gaacttacat tgatcaggca gttgcttcgg gtgtatttac gtcgaatgaa gacgaagcca
       61 tgtttaaata ctccaaagaa gcacccaacg aatgctactt ctaccggggt tattttgtgg
      121 atggacgtaa cgacttttca ataacaaagc cgtttacaga ccttttaaaa ggacagcccg
      181 acactctgaa caacaagcaa catccgtggg aaggcgtagt tgatccgcgg ctatcaattt
      241 acacaacgcc aagaggagga aaataccttg gacttcctta cggactaccg agcaacaaca
      301 tgacaagatc tatcagagac gcagctccct cctggtatcc tgcttctcct cctattattt
      361 taaataagga cttctccgtt ccgttgatga cgtatgcaga attgtgtttc attctcagcg
      421 aacgctacaa ttgggatcaa gaatggtacg aagaaggggt caggtcttcg gttgaacact
      481 ggtatgcact ggcaggtaac gctgttgacc aaactacgtt ggacagctac gttgagtcag
      541 tcagcgaaac cataaacgca gaaactgtgg caacccagaa atacatccat ctctatatgc
      601 agggaaccga agcctggagt gaatatcgga gaaccggcta tcctttaacg atgcttaaac
      661 cgggagaatt tacctatgtg gtaggtgggg aaaaagtggc atttactccg ctttccgaca
      721 caaaaggaga tctacctgcc cgtgttaagt atcctaccaa tgaaagtacg ttaaatccgg
      781 atggttttaa tgcagcaata gccaaacttc aggacggaac caacaactac tattccaaaa
      841 tgtactggga tgtcagatct gaacagaatc cacatccgac caacaaatag gaatgctgac
      901 acgaaagtga ttcaccattt tcaataaaac tatattaaaa ctctatttta acaaggtgcc
      961 ttttcaggca ccttgtttgt tttttctccc gcatatttct tttaaagtgt aatataaaca
     1021 ctccgatttt agagagagta ttacaaataa attgaaataa atacaaaaaa aacaatttgg
     1081 tat
//
